text:
Sign up for our newsletters
Receive the latest in Footwear, Fashion, Music and Creativity in our newsletters.
In this new short from Castrol, Ken Block, Augusto Farfus, Mike Rockenfeller and Adrian Zaugg pit themselves against an obstacle course made of light as they race around in an enviable selection of vehicles. With the help of the talented team at Marshmallow Laser Feast, Castrol was able to create a series of obstacles including the Laser Tunnel, Laser Drift, Slalom, and Laser Box using nothing more than high-powered lights and lasers. Watch as the four drivers tackle the course — and a synchronized jump — in their high-powered sports cars.


text:
People's Rally Converges in D.C. to Celebrate TPP Defeat, Chart Path 'Forward Together'
"I don't care if you're a Clinton supporter, a Trump supporter or a Bernie supporter—we are a movement. It's not about who's in the White House; it's about who's in the streets," NNU Executive Director RoseAnn DeMoro told a crowd of thousands gathered in Washington D.C. today. More »

subset: commoncrawl


text:
If this is your first visit, please click the Sign Up now button to begin the process of creating your account so you can begin posting on our forums! The Sign Up
process will only take up about a minute of two of your time.
Type: Posts; User: mikmarsh
Forum:MarketPlace questions or problem?
Forum:Giant Scale Aircraft - 3D & Aerobatic
Forum:RC Warbirds and Warplanes
Forum:3D Electric Flying!
Forum:Events, Shows and Fun Fly's - Airplanes
Forum:RC Helicopter General Discussions


text:
© Copyright 2017 fallenangel1992. All rights reserved.
Status: Finished
Genre: Romance
don't worry my friend as the chapter was of perfect length and i'm loving the story and characters you have created. the story writing and speech flow nicely too ") i am intrigued by the three pairs of foot prints leaving the house and the lack of real identification of her parents bodies. there is a real mystery unfolding and i am loving it ")
Yikes. I felt sick to my stomach listening to the detective describe what happened to her parents. I don't think I could've sat there with a straight face hearing the horrible death they died. I would be as determined as her to get to the bottom of the cause of the fire and would not rest until justice was brought to those who did it to them. This chapter has me dying for more. I cant wait to find out what happens. KMU
Nicely descripted.Good work Two thumps up..........
Nice chapter :-) looks like a mystery s on da way :-) do kmu
Great chapter man :D
Little advice. If you feel the chapters are too short, they probably are. Unless you want a dramatic impact with a shorter, more important chapter. if you need to take extra time to make it feel the right length for you by all means take it. I love reading but I believe a writer should be able to enjoy their work without biting their nails and worrying about chapter lengths.
Poem / Poetry
Paste the link to picture in the entry below:
Paste the link to Youtube video in the following entry:
Cannot annotate a non-flat selection. Make sure your selection starts and ends within the same node.
An annotation cannot contain another annotation.
There was an error uploading your file.


text:
Pump Shotgun
Redirected from Pump Action Shotgun
this wiki
- "Standard shotgun ideal for short-range combat. A high-projectile spread makes up for its lower accuracy at long range."
- ―GTA V description on the Rockstar Games Social Club.
The Shrewsbury Pump Shotgun is a shotgun appearing in all Grand Theft Auto games since Grand Theft Auto 2.
Grand Theft Auto 2
The shotgun in Grand Theft Auto 2 is used by Special Agents and gang members if their hatred towards the player is high enough. In GTA 2, the shotgun bears some resemblance to a Mossberg 500 with a pistol grip and no stock.
3D Universe
A unique quality attributed to the shotgun is that it fires several projectiles at once, and at close range two accurate spreads will destroy most non-bulletproof vehicles. The shotgun pellets can also knock down enemies. Another advantage is that it can be purchased at most Ammu-Nation stores for a cheap price or found in Police Vehicles, making it both powerful and inexpensive.
A large disadvantage with this shotgun is that the player cannot run with it in GTA III, GTA: Vice City and GTA: San Andreas. Also, the pump mechanism has a very slow rate of fire, so it cannot be used effectively against multiple enemies.
In Grand Theft Auto III, the shotgun's in-game model is a Remington 870 with a Police Folding Stock. While obscured in game in GTA: Advance, the shotgun icon for GTA III was reused for Advance, implying the shotgun in GTA Advance is similar to that of GTA III. For Vice City (where it is known as the "Chrome Shotgun") and San Andreas, it resembles an Ithaca 37 with a wooden buttstock. For Liberty City Stories and Vice City Stories, it is an "Over and Under" shotgun. However, it functions like a pump shotgun.
HD Universe
- "One shell from this single-barreled gentleman's firearm should provide enough punch to knock anyone down."
- — Description in GTA CW.
In Grand Theft Auto IV, the Pump Action Shotgun is an Ithaca Model 37 Stakeout, though is based on the Auto Hardball Model 870 Airsoft variant. Its design is similar to the Stubby Shotgun. The shotgun holds eight shells, although with the length of the barrel and magazine tube it should only hold four.
The Pump Shotgun can be purchased from the back-alley gun shops for $1200, and each additional magazine is worth $100. The Pump Shotgun is not available from Little Jacob. The LCPD, NOOSE, and FIB, in addition, use this gun as a secondary weapon. It can be picked up from them when they are killed. This gun, rarely used on Multiplayer but extensively used on Single-Player, is extremely powerful. It will kill any Single-Player foes with one shot (even fully healthy fully armored ones at point blank), and the most needed for Multiplayer will be about 3 or 4 leg shots. This is a one-hit kill at close range on any part of the body. It cannot be fired from cars. Members of the Russian Mafia and biker gangs will sometimes have it as a secondary weapon.
The Pump Action Shotgun in Grand Theft Auto V is modeled after the Mossberg 590 Cruiser, with several modified parts such as the pistol grip housing that accepts AR-15 pistol grip, and the stock that accepts M4-styled stocks, which in this case, is the Magpul MOE stock.
GTA V Overview
|Rate of fire
(Rounds per minute)
|Reload speed
(Time taken to reload)
|Damage per shot
|Effective range
1.76 seconds
Customizations (GTA V)
|This article or section contains insufficient information and is considered as a Stub. You can help the GTA Wiki by expanding it as much as you can.
Requiring improvement: Next-Gen and Online Prices, Clip Sizes and Prices
|Rounds & magazine
|GTA Online (Unlock)
|Cartridges (16)
|Flashlight (provides ilumination when aiming)
|Suppressor (silences but slightly reduces damage and range)
|$5000 (Kill 100 players)
|Green (Collector's and Enhanced Edition)
|$7500 (Kill 200 players)
|Orange (Collector's and Enhanced Edition)
|(Kill 400 players)
|$5750 (Kill 600 players)
|Pink (Collector's and Enhanced Edition)
|$7500 (Rank 50)
|Gold (Collector's and Enhanced Edition)
|$10000 (Rank 75)
|Platinum (Collector's and Enhanced Edition)
|$12500 (Rank 100)
In the 3D Universe and HD Universe, Police Cars will have a shotgun with five rounds in them, which is added to the player's arsenal once they enter the vehicle. In GTA IV Police Cars will instead contain a Combat Shotgun.
Grand Theft Auto III
- Saint Mark's, Portland Island - On top of one of the car garages on the block north of Momma's Restaurante. You have to go through the rooftop park across the street west of Salvatore's Mansion to gain access.
- Hepburn Heights, Portland Island - On the northwest side of the eastern tall apartment building.
- Rockford, Staunton Island - Available to buy from Phil Cassidy's Army Surplus after completion of Arms Shortage ($1,500).
- Belleville Park, Staunton Island - Behind the obelisk on the east side of the main park.
- Bedford Point, Staunton Island - North of the pier west of Liberty Tree Offices.
- Cochrane Dam, Shoreside Vale - At the base of the Cochrane Dam power house, near the road loop, on a metal ledge on the east side.
- Saint Mark's, the Leone Family gang carry this weapon for the rest of the game, after the mission Sayonara Salvatore. This makes subsequent visits to this area especially dangerous, as the player's unarmoured vehicle can sucumb to a few shotgun blasts, while on foot the player can be knocked down by shotgun pellets and then swarmed.
- Spawns at each safehouses after collecting 40 Hidden Packages.
- Automatically given to the player by entering a 4-door Police vehicle.
Grand Theft Auto: Vice City
- Washington Beach - Behind one of the benches on the sidewalk by the beach south of Standing Vice Point hotel.
- Ocean Beach - On the top parking level (southeast side) of the Washington Mall.
- Starfish Island - In the small room at the bottom of the Mansion's stairwell, east of the main entrance after completion of Rub Out.
- Automatically given to the player by entering a 4-door Police vehicle.
Grand Theft Auto: San Andreas
- In the Los Santos Police Department. However, if you have a gun equipped in a police station, a 2-star wanted level is obtained. It is recommended to have a Melee weapon equipped upon entering a police station.
- Dillimore, Red County - Inside the police station.
- Doherty, San Fierro - At the southeast side of Solarin Industries.
- Doherty, San Fierro - Spawns outside of the Doherty Garage upon photographing all 50 Snapshots.
- Valle Ocultado - On a dock, not too far from a Skimmer.
- Fort Carson, Bone County - In front of the third most eastern house of the outskirt of Fort Carson, more exactly, on the green area. Note that the first and second most eastern houses are almost on the same longitude.
- Pilson Intersection, Las Venturas - At the right side of the intersection, under the overpass.
- In the Las Venturas Police Headquarters.
- Automatically given to the player by entering a 4-door Police vehicle.
Grand Theft Auto: Liberty City Stories
- Portland View, Portland - At top of the Fidl supermarket
- Harwood, Portland - Spawns on a pier near the ferry station.
- Bedford Point, Staunton Island - In an alleyway behind the LCFR headquarters.
- Spawns at each of the player's safehouses after collecting 20 Hidden Packages.
Grand Theft Auto: Vice City Stories
- Little Haiti - Spawns on a roof across Le Singe d'Arbre.
Grand Theft Auto IV + Episodes from Liberty City
- Meadows Park - Can be found on the second decrepit pier to the right of Sing Sing Ave/Dukes Bay Bridge, over the water.
- Francis International Airport - Can be found next to a support column for the Broker-Dukes Expressway on-ramp. The support column is the first one the player sees on the right, from the airport entrance.
- Northern Gardens - Can be found over the cliff on the north side of Grand Boulevard. The shotgun is lying next to a large bush.
- Middle Park - Can be found on the C/B platform of the C/K Algonquin Inner Line at North Park Station.
- Varsity Heights - Can be found by two truck trailers in a construction equipment storage depot, to the right of Union Drive West. The shotgun is laying on the dividing wall.
- Alderney City - Can be found in the vacant lot behind the AutoEroticar lot.
Grand Theft Auto: Chinatown Wars
- Can be bought from Ammu-Nation after the mission Carpe "Dime"
- Found in a construction side near Francis International Airport.
- Awarded by entering a police car (once per car).
Grand Theft Auto V
- In several structures found under water, usually near the locations of Submarine parts.
- Used by LSPD officers, NOOSE officers, Military inside Fort Zancudo, and Sheriff troops in Blaine County.
- Shop clerks may pull out this shotgun if threatened in any food shop around Los Santos.
- Awarded to the player by entering a Police Cruiser, Sheriff Cruiser, Police Buffalo or Interceptor.
In-game model
FPS Gallery
HUD icons
There is a glitch in GTA V if you blind fire the sawed off shotgun it will be pumped after the shot then be pumped again when the gun goes back in cover which in reality would waste a shell.
There is a glitch in GTA IV that if you keep crouching and shooting at the same time, the weapon will become automatic but more powerful than the Combat Shotgun. There is also another glitch with this shotgun, in which the player can make it fire very rapidly, all eight shells can be fired in around the same time it takes to fire two normally.
- In the 3D Universe, it is possible to shoot the Pump Action Shotgun without pumping it. After shooting the Pump Action Shotgun, jump quickly and the animation of the player character pumping the Pump Action Shotgun will be skipped. In GTA: San Andreas, the player must crouch, shoot while crouching and then stand up, causing the pumping animation to be skipped, though the sound of the pump is still heard.
- In the 3D Universe, the Pump Action Shotgun does not need to be reloaded; it just needs to be pumped.
- In the 3D Universe, a shotgun typically fires three pellets that travel in three visible lines. In the HD universe, the shotgun more realistically scatters pellets in random trajectories, rather than rigid lines.
- The Pump Action Shotgun in GTA III is equivalent to the Stubby Shotgun in later GTA games, in that the shotgun shoots five pellets per shell, kills in one shot at close range and can destroy a car in two shots, which makes the GTA III shotgun and the Stubby Shotgun roughly the same in terms of stats.
- In GTA III, when passing trough the mafia territory after the mission Sayonara Salvatore, the mobsters wield shotguns as well, but when they shoot at a car, which in the player will be (otherwise, they wouldn't shoot at a vehicle if the player isn't in it), the car would explode from one shot. This making impossible to drive a vehicle in Mafia territories.
- In the IOS version, the player can sometimes fire multiple shots without pumping the shotgun by hitting the fire button immediately after firing. If a pedestrian is in range, auto-aim will lock onto it and the gun will fire, skipping the pumping animation. Alternatively, If a pedestrian bumps into Claude as he is pumping the shotgun, the animation will be skipped, allowing you to fire again immediately.
Grand Theft Auto: Vice City
- In early screenshots and trailers for GTA: Vice City, the Pump Action Shotgun had the same model as the Pump Action Shotgun from GTA III, but it was later changed. It is still hidden in the files.
- In the original PS2 version, the weapon model has a shorter magazine tube.
GTA San Andreas
- The Pump Action Shotgun in GTA: San Andreas ejects a shell when CJ fires the weapon, which is illogical, as the shell should only come out once he pumps the shotgun after firing.
- Before reaching "Gangster" skill level with the Pump Action Shotgun, Carl fires the shotgun without utilizing the stock.
- In GTA San Andreas, if the cheat code ammo never runs out "FULLCLIP" is activated, then the pumping sound will not be heard, although the animation where CJ pumps the shotgun will still be played.
Grand Theft Auto IV
- The GTA IV rendition of the shotgun bears a "MODEL No. 69" etching (alongside another reading "~ 12.GAUGE ~") which is under the other reading.
- The Ithaca 37 "Stakeout" shotgun is considered to be a Stubby Shotgun in the 3D Universe. However, it is called the "Pump Action Shotgun" in GTA IV, and acts like one.
GTA V/Online
- The Pump Shotgun is favored during GTA Online Heists, as it is used during The Fleeca Job and The Humane Labs Raid.


text: Bask in the beauty of these stunning earrings, which feature a glimmering drop bejeweled with oval-cut London blue topaz (5-1/2 ct. T. W. And round-cut white topaz (1/8 ct. T. W. Set in sterling silver. Approximate drop: 1/4 inch. Disclaimer: Photo may have been enlarged and or enhanced. Color: metallic


text:
More Videos...
1969 cougar that we made into an eliminator
1969 cougar right after we added lotts of new modds
Muscle Car Of The Week Video #37: 1969 Mercury Cougar XR7 Cobra Jet 428
In 1969, you could walk in to your local Mercury dealer and rumble out behind the wheel of a Cougar XR7 Cobra Jet 428 and win street races without attracting the attention of the local fuzz. The '69 XR7 CJ428 is a 335 HP / 440 TQ tire-burner with subtle European design flair. The Brothers Collection is home to many bright and loud Muscle Cars, but this one is an example of speaking softly and carrying a big V8!
69 Cougar XR7
My 69 Cougar XR7 - for sale. Original 351W, FMX tranny, and Ford 9" rear. Rebuilt tranny Rebuilt rear end (373 gears) Hooker headers Full Flowmaster American Thunder dual H-pipe Exhaust All new blueprinted front suspension with 1" drop New steering sector components All new rear suspension - including leaf springs Disk brake conversion - 4 piston Mustang setup. New brake lines and master cylinder all the way around Holley street avenger 650 carb and intake setup Lokar cabling setup New electronic points, ignition module Modern power headlight conversion from Kens Cougars New vacuum primary New hoses New Vacuum actuator for headlight doors New turn signal for tail lights New sequential lights box few other odds and ends. Needs new roof cap, the front seats reupholstered, and a headliner.
Rusty Roof Replacement - 1968 Cougar
If you have an old car that came with a vinyl top, chances are that you have some rust issues in the roof. This was the case with Andrew's car - a 1968 Mercury Cougar that his grandparents bought brand new in '68. In this video, Andrew takes matters into his own hands to replace his rusted roof skin with a solid donor roof from another car. He is by no means a restoration expert, just an amateur trying to save some money and save his car. Need a roof or other body panels for your classic Cougar? West Coast Classic Cougar has it all. Check us out at http://www.cougarpartscatalog.com or give us a call at (503) 463-1130 . (c) 2011 West Coast Classic Cougar, Inc. Music in the video is from royalty-free sources, except for the Toccata and Fugue in D (used during the brief B&W horror sequence). Performed by Peter Hurford. An advertisement appears on this video in order to pay royalties to the copyright holder, not for our own gain.


text:
iFixit Stories May 11, 2016
iFixit Stories April 4, 2016
It's been a year and a half since iFixit tore into the flagship 9.7-inch tablet from Apple, and now it's finally time to look inside the smaller iPad Pro and see what it's really made of. What you'll find is your typical mix of components from Samsung, Bosch Sensortec, NXP Semiconductors, and others, plus a whole lot of battery. Cutting to the chase, iFixit rates the smaller iPad Pro's repairability at a low 2 out of 10, which is the same as the iPad Air 2 it replaces but one point below what it rated the 12.9-inch iPad Pro last year. Here's what's inside the 9.7-inch iPad Pro and why iFixit says it's not so repairable…
iFixit Stories November 24, 2015
The iPad Pro accessories, Apple Pencil and Smart Keyboard, may be in short supply worldwide but that doesn't stop iFixit from tearing them down. The Apple Pencil teardown demonstrated some clever internal component miniaturization and the Smart Keyboard has similar tricks. Most notably, the teardown shows Apple's "conductive fabric material" that runs down the length of the accessory. Rather than trailing wire or a ribbon cable inside the keyboard, Apple is using special fabric that can send power and data in both directions. iFixit believes this has durability benefits too, claiming it should be less likely to break than normal wiring would over the course of the product's lifetime.
In fact, in general, the Smart Keyboard is praised for its durability here. The entire product is encased in a rich fabric for water and stain resistance leaving no visible holes; iFixit literally had to cut their way inside the keyboard. Inside, there are a lot of protective layers to keep the components in place. The less good news is that iFixit scored the Smart Keyboard a 0/10 for repairability as any attempt to get to the internals involves damaging the device permanently.
iFixit Stories November 19, 2015
iFixit Apple Pencil teardown reveals twin emitters to measure angle & orientation, logic board folded in half
No new Apple tech survives long without being taken apart by iFixit, and the Apple Pencil is no exception. Apple didn't make it easy, however, with iFixit having to resort to a small circular saw to open up the device and needing to break a flex cable along the way.
Inside, it found that the plastic outer casing reveals a metal inner one. The tip contains two separate emitters, used to determine both the angle and orientation of the Pencil in order to adjust the width of the pen stroke. With such little space to play with, Apple had to get creative with the logic board … expand full story
iFixit Stories November 11, 2015
It's no surprise that the people over at repair guide portal iFixit have already started their ritual teardown of Apple's new iPad Pro. The new device features a larger 12.9-inch display, the 3rd generation 64-bit A9X chip, and other upgraded internals, but are there any other new surprises inside the iPad Pro?
iFixit Stories October 16, 2015
Apple debuted new versions of its Mac peripherals on Tuesday with the Magic Mouse 2, Magic Keyboard, and Magic Trackpad 2, replacing the aging AA battery-powered predecessors with versions that use Lightning cables to recharge built-in batteries. We've already seen hands-on unboxings for anyone interested but not curious enough to dish out the $330 total for all the new Magic gear, and now iFixit has followed up with the usual teardown and repairability analysis for each new Apple accessory. expand full story


text:
Our aim is to create for you a stress-free ski or cycling holiday, in the picturesque ski village of Les Carroz, near the popular resort of Flaine, in the Grand Massif ski area of France. We have our own catered chalet, Chalet Andelain, and we are now taking bookings for Summer 2013 and Winter 2013/14.
Chalet Andelain is our home in this stunning location which has magnificent unspoilt views of the surrounding mountains. We are situated a 5 minute drive from the main ski lift, so as we have our own minibus we can easily and quickly transport you up to the village of Les Carroz to access the telecabine, or the rest of the village facilities.
Your hosts, Maurice & Nicola
We ran a business in the UK for 20 years, selling and servicing plastic moulding machines. Before this Maurice was a service engineer and Nicola a radiographer. We bought an apartment in Les Carroz back in 2002 and as we were spending more and more time in the village we decided in 2006 to live here permanently.
Maurice has been a cyclist for more than 30 years and as we were living on Cannock chase he slipped easily into mountain biking some 20 years ago. He had his first winter-sports holiday in 1995 and became completely hooked on boarding and skiing. Having skied in many of the European ski resorts we finally found Les Carroz and completely fell in love with the area, deciding it was the best place we had ever skied. With Maurice's passion for skiing and cycling and Nicola's passion for cooking, it was an easy decision to buy and run our own catered chalet.
Maurice & Nicola


text:
A to Z of Destinations
Australia, NZ and South Pacific
Latin America
Middle East
North America
A to Z of Experiences
Walking and trekking
Diving and snorkelling
Wildlife and safaris
Meet the locals
Frontier and expedition
Travel icons
Cycling and Mountain Biking
Boat trips
Solo travel
Visiting the Poles
Outdoor Activities
Career breaks and BIG trips
Rail journeys
Body and soul
Short breaks
Volunteer and conservation
Family travel
Horse riding
Sri Lanka
United Kingdom
Australia, East Coast
Australia, West Coast
Everest Base Camp
Silk Road
Annapurna Circuit
Trans-Siberian and Trans-Mongolian railway
Dog sledding
Machu Picchu and the Inca Trail
Aurora Borealis/Northern Lights
Cruising the Nile, Egypt
Ice Hotel
Climb Mount Kilimanjaro
A to Z of Destinations
Australia, NZ and South Pacific
Latin America
Middle East
North America
A to Z of Experiences
Walking and trekking
Diving and snorkelling
Wildlife and safaris
Meet the locals
Frontier and expedition
Travel icons
Cycling and Mountain Biking
Boat trips
Solo travel
Visiting the Poles
Outdoor Activities
Career breaks and BIG trips
Rail journeys
Body and soul
Short breaks
Volunteer and conservation
Family travel
Horse riding
Neither Africa nor Asia, Madagascar defies classification. From desert to rainforest, mountain to beach it's packed with endemic species and a culture all of its own
Adrift in the Indian Ocean, the size of France but with about ten main roads, Madagascar is one country where you can really escape the influence of the modern age and escape to remote communities scarcely touched by the outside world. Increasingly it is being discovered by naturalists and anthropologists as well as those who value its remote beaches.
Madagascar's history is key to its development. The island split from Gondwanaland before big predators had developed. Instead of primates Madagascar developed lemurs, a gentle, unaggressive near-monkey, whose nearest relative lives in South America. There are more than 50 species now, but all have gentle hands and soulful eyes.
A stunning 90% of Madagascar's flora and fauna are endemic, found nowhere else in the world. These are protected in National Parks where rugged hikes are needed to track rare species and in private reserves where, to appeal to the French market, the lemurs are fed from the table and almost tame.
Its isolation also shaped the island's culture. The human gene pool arrived by boat from about 500BC, crossing the Mozambique Channel from Africa and drifting across the Indian Ocean from Southeast Asia and Austronesia. Here they blended and divided into 18 different tribes, each with its own language and beliefs.
Village life is conducted under a strict set of taboos and traditional beliefs. The island's musical traditions also developed independently: each region has its localised types of music, keenly followed live and on bootleg VCR tapes. Two hundred years of French colonisation have overlaid a thin (but fervent) layer of Christianity and basic cooking skills but never threatened local cultures and beliefs.
All this and natural beauty too. Straddling climate zones Madagascar has rocky wastes of spiny desert, patches of montane and tropical forest, intensively-farmed cool highlands and vanilla-scented lowlands basking in year-round warmth. Life continues offshore: cross the golden-sand beaches and join the lateen-rigged wooden fishing boats: below the surface are little-dived coral reefs and big pelagics.
Wanderlust recommends
Wanderlust tips
Learn some French – but don't get too fluent. The Malagasy are generally friendly but open up once they realise you're not from France: most cordially dislike their colonial overlords. Only the young have had the opportunity to learn English and – unless you're prepared to tackle one of the 18 regional dialects – communication relies on a broad-vowelled version of school-room French.
30th April 2016
They take some getting to, but adventurer Ash Dykes says these remote corners of Madagascar will reward you ten-fold.
29th October 2015
Inspire Me
Don a mask and fins and ready yourself for the ten best reef encounters in the world...
8th May 2015
Untamed and largely untrammelled, Madagascar offers the adventurous traveller stunning landscapes, unique wildlife and intriguing encounters. What are you waiting for?
Venezuelans skate to church. Norwegians hide their brooms. And Catalans beat the hell out of dressed up logs. Christmas really does drive people crazy...
The Cagols have just spent a year travelling the world. 11-year-old Madeline shares her diary entries from their last week in Africa, revealing what she really thought about her family's big adventure.
Jarryd Salem, one half of travel-blogging couple NOMADasaurus, outlines the ways travelling together can make a couple stronger and happier
From travel writing and photography to being a pilot or working with animals, getting paid to travel is many people's dream job. Here, 12 experts in their fields tell you the highs, lows and what it takes to succeed…
All set for the weekend? Before it gets here, allow yourself a bit of monkey business and let us know your most hilarious caption for this pic for a chance to win prizes
Best country. Best city. Favourite airline. Top TV show. Best tour operator. Wanderlust's readers have spoken, in the Wanderlust Reader Travel Awards 2017, and here are the results…
Simply select the destination you're interested in or the activities you're looking
for and we'll send your request to a select panel of tour operators.
Each operator will respond to your request individually. Your details remain private
and are not disclosed to any partners unless you decide to proceed with a booking.
Up to £300 discount to travel Bangladesh
18 Days in Bangladesh from £1,590 per person or less
20% off Norway Arctic Expedition Cruise - Save up to £1,100!
Wanderlust sends out regular email newsletters – be the first to know about web
exclusives, competitions, hot offers and travel jobs. Register today!
I have read and agree to the Terms &
Where in the world are you? Add
#wanderlustmag to your tweets and share your latest travel adventures with
fellow Wanderlusters on wanderlust.co.uk
Get to know Wanderlust on facebook and bring all your travel-minded friends, too


text:
Pacific Fire Shrimp
Total Time
Prep 15 mins
Cook 5 mins
On the bay in San Diego there is a place called Peohe's on the waterfront that offers a great view of the downtown area of San Diego. This dish is still being served today. It can be a appetizer or entree!
Ingredients Nutrition
- 226.79 g butter, softened
- 14.79 ml creole seasoning
- 7.39 ml lemon pepper
- 7.39 ml rosemary
- 7.39 ml Tabasco sauce
- 7.39 ml lemon juice
- 907.18 g shrimp, peeled devined
- Preheat oven to 400. Mash the butter with the Creole seasoning,lemon pepper,rosemary,Tabasco sauce and lemon juice.
- Arrange shrimp in a baking dish and brush on the seasoned butter. Bake for 5 to 6 minutes. Serve hot. French bread goes well with this on the side.
- This can be made in advance, spread on the butter seasoning, refrigerate up to 8 hours. Heat and serve.
Most Helpful
Very unique way to bake shrimp! No garlic! I'd suggest if you don't love rosemary to reduce the amount.....luckily I love rosemary! This isn't hot at all, despite the "fire" in the name. And the bread is a delicious addition. Really enjoyed this wonderful shrimp appetizer. Thanks for sharing!


text:
1990 Mercedes-Benz 560SEC QuestionsRefine by vehicle
Upper Line of Leveling Valve leaks hydro fluid. Some wear from the bar and also a small crack in the line. Rust on the line connector. Soaked in Kroil. Recommend to use heat or not ?
Would a new leveling Valve be best?
The lights on the switch for the heated seats occasionally go on but even when they do the seats won't warm up wondering if it is a switch issue or other problems
all of my dash lights come on and my gauges are bouncing all over the place
I checked fuses they are not blown, what should I look for next? Any suggestions? Since both front seats can not be adjusted I am wondering if a wiring issue or module may be out. Where can I go in the car to check?
Is it possible to drop in a modern AMG engine into a 560sec (or any model from 2000 motors kompressor
or any new engineer from 2004 kompressor to 560sec
my 1986 mercedes 560 sec gas gauge is stuck on full, the speedometer is not correct read 65 when I am doing more like 50
Just bought the car. Had high idle and ABS light on. Found bad fuse for that and brought idle to normal. Runs good, oil pressure very good. After about 5 miles of driving it all of the sudden started smoking. Looks blue to me but could be lighter color. Any ideas why it didn't smoke before or what could have caused it to start smoking now or what to do to fix it? Drove the car several times prior to fixing idle and had no indications of smoking at all. Even now seems to run fine, gauges all look good, just smokes bad. Thanks for the help
Whenever I start driving the car, it doesn't accelerate properly.... I have changed almost everything and no luck yet.. any idea ??
As soon as I let go of the ignition key, the engine will die. As long as I keep the key cranked the vehicle will run, any suggestions??
the hood won't open and i can't see the latch
Where on the engine is the fuel filter located, and, is it easy to change
My car is a 1987 560 sec mercedes benz and I can't get the idle fuse out to change it . My car idles really fast and I was told to change the fuse . I can't get the fuse housing for the fuse open .Thank you , Debbie
No blower operation at any speed, blower motor is good. I need to locate the blower control module to test it and its wiring. Where is it located?


text:
Jason Falk, senior applications engineer at Hurco North America talks about the company's 3D printing and CNC machining technology.
CEO of John Force Racing Inc. (JFR) and 16-time NHRA Funny Car champion John Force along with Cory Miller, General Manager of Hurco North America, signed an agreement Tuesday that will benefit the two Indianapolis area based companies. JFR will add six state-of-the-art CNC machines from Hurco that will continue to enhance the development and production capabilities of JFR and its Force American Made (FAM) machining company.
Josef Newgarden drove the Hurco car straight into the winner's circle at the Grand Prix of Alabama race. Read details and see pictures at the links provided.


text:
NF-κB inhibitor dehydroxymethylepoxyquinomicin suppresses osteoclastogenesis and expression of NFATc1 in mouse arthritis without affecting expression of RANKL, osteoprotegerin or macrophage colony-stimulating factor
DOI: 10.1186/ar2298
© Kubota et al., licensee BioMed Central Ltd. 2007
Received: 13 June 2007
Accepted: 25 September 2007
Published: 25 September 2007
Inhibition of NF-κB is known to be effective in reducing both inflammation and bone destruction in animal models of arthritis. Our previous study demonstrated that a small cell-permeable NF-κB inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ), suppresses expression of proinflammatory cytokines and ameliorates mouse arthritis. It remained unclear, however, whether DHMEQ directly affects osteoclast precursor cells to suppress their differentiation to mature osteoclasts in vivo. The effect of DHMEQ on human osteoclastogenesis also remained elusive. In the present study, we therefore examined the effect of DHMEQ on osteoclastogenesis using a mouse collagen-induced arthritis model, and using culture systems of fibroblast-like synovial cells obtained from patients with rheumatoid arthritis, and of osteoclast precursor cells from peripheral blood of healthy volunteers. DHMEQ significantly suppressed formation of osteoclasts in arthritic joints, and also suppressed expression of NFATc1 along the inner surfaces of bone lacunae and the eroded bone surface, while serum levels of soluble receptor activator of NF-κB ligand (RANKL), osteoprotegerin and macrophage colony-stimulating factor were not affected by the treatment. DHMEQ also did not suppress spontaneous expression of RANKL nor of macrophage colony-stimulating factor in culture of fibroblast-like synovial cells obtained from patients with rheumatoid arthritis. These results suggest that DHMEQ suppresses osteoclastogenesis in vivo, through downregulation of NFATc1 expression, without significantly affecting expression of upstream molecules of the RANKL/receptor activator of NF-κB/osteoprotegerin cascade, at least in our experimental condition. Furthermore, in the presence of RANKL and macrophage colony-stimulating factor, differentiation and activation of human osteoclasts were also suppressed by DHMEQ, suggesting the possibility of future application of NF-κB inhibitors to rheumatoid arthritis therapy.
Prevention of bone destruction in affected joints is one of the most important goals in the treatment of rheumatoid arthritis (RA), and many clinical trials of newly developed biologic agents include assessment of radiographic changes before and after treatment. For example, a significant effect of anti-TNF therapy in halting the progression of joint structural damage in active RA has been reported [1–3]. There are still some patients with persistently active disease, however, despite the use of currently available agents; further development of small, cell-permeable agents that specifically interrupt the critical intracellular pathways involved in bone destruction could prove beneficial.
Recent studies have revealed the prominent contribution of osteoclasts to bone resorption that may be dissociated from inflammation in RA pathophysiology. For example, human TNF transgenic mice were protected from bone destruction despite severe arthritis when they were crossed with c-fos-deficient mice lacking osteoclasts [4]. In early RA patients treated with methotrexate and infliximab, radiographic progression was slowed even in cases with elevated time-averaged levels of C-reactive protein or erythrocyte sedimentation rate or elevated time-averaged swollen joint counts [3]. Osteoclasts are multinucleated cells formed by fusion of mononuclear progenitors of the monocyte/macrophage lineage. The osteoclasts develop a specialized cytoskeleton that permits them to establish an isolated microenvironment between themselves and the underlying bone, within which matrix degradation occurs by a process involving proton transport to acidify the extracellular microenvironment [5]. Acidification of this compartment leads to the activation of tartrate-resistant acid phosphatase (TRAP) and cathepsin K, which are the enzymes responsible for degradation of bone mineral and collagen matrices [6].
NF-κB is a transcription factor implicated in diverse receptor-mediated signaling pathways including differentiation and activation of osteoclasts [7, 8]. Several lines of in vitro and in vivo studies have demonstrated that inhibition of NF-κB results in suppression of osteoclastogenesis [9–12]. As regards mechanisms underlying the involvement of NF-κB in osteoclastogenesis, Takatsuna and colleagues [12] demonstrated that expression of NFATc1, a key transcriptional factor of osteoclastogenesis induced by macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-κB ligand (RANKL) in a culture of murine precursor cells [13], was inhibited by the NF-κB inhibitor dehydroxymethylepoxyquinomicin (DHMEQ).
DHMEQ is a unique NF-κB inhibitor designed in our laboratory based on the structure of the antibiotic epoxyquinomicin C, which acts at the level of nuclear translocation of NF-κB [14]. An in vivo anti-inflammatory effect of DHMEQ has already been demonstrated in various models, including collagen-induced mouse arthritis [15–17]. Since inflammation and bone resorption could be considerably dissociated as mentioned above, and many factors besides RANKL and M-CSF are thought to affect osteoclastogenesis [18], the effect of DHMEQ on in vivo osteoclastogenesis needed further investigation. In the present study, therefore, we looked into the effect of DHMEQ focusing on in vivo osteoclastogenesis in collagen-induced arthritis. In addition, we tested the effect of this compound on human osteoclast differentiation in vitro, to explore the possibility of future development of novel RA therapy.
Materials and methods
Inhibitor of NF-κB
The (-)-enantiomer of DHMEQ, which is simply represented as DHMEQ in this manuscript, is a more potent inhibitor of NF-κB than its (+)-enantiomer, and was synthesized as described previously [19].
Induction of collagen-induced arthritis
Animal experiments were approved by the Institutional Animal Care and Use Committee of Tokyo Medical and Dental University. Male 8-week-old DBA/1J mice were purchased from Oriental Yeast (Tokyo, Japan). Bovine collagen type II (Collagen Research Center, Tokyo, Japan) was dissolved in 50 mM acetic acid at 4 mg/ml and was emulsified in an equal volume of Freund's complete adjuvant (Difco Laboratories, Detroit, MI, USA). Mice were immunized with 100 μl emulsion intradermally at the base of the tail. After 21 days (day 0), the same amount of the antigen emulsified in the same adjuvant was intradermally injected at the base of the tail as a booster immunization. From day 0 to day 10, 100 μg DHMEQ (5 mg/kg body weight) dissolved in 50 μl dimethyl sulfoxide was injected subcutaneously every day to mice in the experimental group. Mice in the control group received 50 μl dimethyl sulfoxide similarly injected.
The thickness of each hind paw was measured on day -4 and on day 10 using a pair of digital slide calipers. Radiographs of both ankle joints were obtained on day 10 and were scored on a scale of 1–3 (1 = no change, 2 = mild osteoporosis without bone erosion, 3 = severe osteoporosis with or without bone erosion) by three investigators who were blinded to the assignment of mouse groups.
Histochemical staining of osteoclasts
Mice were sacrificed on day 10, 3 hours after the last injection, and their hind paws were excised for experiments. After the skin was scarified with a surgical blade, the left hind paws were preserved in 10% buffered formalin for 3 hours, and the skin was totally removed. The paws were then decalcified in 10% ethylenediamine tetraacetic acid, 5% polyvinylpyrolidone, 100 mM Tris (pH 7.4) for 4 weeks, were dehydrated in graded ethanol, were permeated serially by methyl benzoate and benzene, and were embedded into paraffin in a vacuum oven. Longitudinally sectioned paraffin blocks were fixed in citrate-acetone (2:3 mixture of 380 mM citrate and acetone) for 30 seconds, and were stained with 0.5 mg/ml naphthol AS-BI phosphoric acid and 0.3 mg/ml fast red violet LB salt (Sigma-Aldrich, St Louis, MO, USA) in 27 mM sodium tartrate and 100 mM sodium acetate (pH 5.2) for 1 hour at 37°C. Nuclei were stained with hematoxylin. The mean number of TRAP-positive giant cells with four or more nuclei in the individual ankle joints of arthritic mice was counted under a microscope by two investigators in a manner blinded to the assignment of mouse groups.
Immunohistological staining of NFATc1 and NF-κB
The right hind paws were frozen in liquid nitrogen, and bone-containing sections were prepared using Cryofilm (Finetec, Tokyo, Japan) [20]. Phycoerythrin-labeled anti-NFATc1 monoclonal antibody 7A6 was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monoclonal antibody to the nuclear localization signal in the p65 subunit of NF-κB was purchased from Chemicon (Temecula, CA, USA), was labeled with FITC (Wako, Tokyo, Japan), and was dialyzed against 100 mM Tris, 200 mM NaCl (pH 7.4). After fixing with acetone for 10 min, permiabilization with PBS containing 0.5% Triton X-100 for 10 minutes, and blocking with PBS containing 10% FCS for 1 hour, the sections were incubated with a mixture of the two antibodies for 1 hour, and observed under a confocal microscope (Olympus, Tokyo, Japan).
Measurement of soluble RANKL, osteoprotegerin and M-CSF
Serum samples were obtained at euthanasia and the concentrations of soluble receptor activator of NF-κB ligand (sRANKL), osteoprotegerin(OPG) and M-CSF were measured by ELISA. The ELISA kits for sRANKL and OPG were purchased from Biomedica (Vienna, Austria), and the ELISA for M-CSF was from R&D Systems (Minneapolis, MN, USA).
Estimation of RANKL and M-CSF expressed by fibroblast-like synovial cells obtained from patients with RA
Synovial tissues were obtained at the time of total knee joint replacement from six patients with RA; these patients were female, aged (mean ± standard deviation) 67.3 ± 9.1 years, and their serum C-reactive protein levels were 3.4 ± 2.4 mg/dl. Of the six RA patients, five women took prednisolone, four women took methotrexate, two women took bucillamine, and one woman took leflunomide. Signed consent forms were obtained prior to the operation, and the experimental protocol was approved in advance by the Ethics Committee of Tokyo Medical and Dental University. RA was diagnosed according to the criteria of the American College of Rheumatology [21].
RA fibroblast-like synovial cells (FLS) were prepared from the synovial tissues as described previously [22], and were cultured in DMEM with heat-inactivated 10% FCS (Sigma-Aldrich). After incubation with or without DHMEQ for 24 h, RA-FLS were collected and lysed with RIPA lysis buffer (Upstate, Lake Placid, NY, USA). After debris was eliminated by centrifugation, 5 μg proteins in the supernatant were separated by 10% SDS-PAGE under reducing conditions, and were transferred to a polyvinylidene difluoride membrane. The membranes were blocked with 4% Block Ace (Snow Brand Milk Products, Sapporo, Japan) in PBS containing 0.1% Tween 20 overnight, were incubated with anti-RANKL monoclonal antibody 70513 (R&D Systems) or with anti-β-actin monoclonal antibody AC-15 (Sigma-Aldrich) in PBS containing 0.1% Tween 20 with 0.4% Block Ace for 1 hour, and were then incubated with peroxidase-conjugated rabbit anti-mouse IgG (Dako Cytomation, Carpinteria, CA, USA) for 1 hour. The immunoblots were detected by enhanced chemiluminescence (Amersham Pharmacia Biotech, Piscataway, NJ, USA). To analyze M-CSF production by RA-FLS, cells (2 × 104/well) were cultured in 96-well plates with or without DHMEQ for 24 hours. The culture supernatants were collected and the concentration of M-CSF was measured using an ELISA kit (BioSource, Camarillo, CA, USA).
Estimation of human osteoclastogenesis and production of matrix metalloprotease-9
Peripheral blood mononuclear cells from healthy donors were collected by Ficoll-Conray gradient centrifugation, and monocytes were positively selected using MACS microbeads (Miltenyi Biotec, Auburn, CA, USA). The monocytes (5 × 104/well) were incubated in 96-well plates in αMEM with heat-inactivated 10% FCS (Sigma-Aldrich), 25 ng/ml M-CSF (Peprotech, Rocky Hill, NJ, USA) and 40 ng/ml RANKL (Peprotech). The indicated concentration of DHMEQ was added throughout the culture period. On day 3 the medium was replaced with fresh medium. After incubation for a further 4 days, the number of TRAP-positive multinucleated cells with three or more nuclei was counted under a microscope. To analyze the expression of matrix metalloprotease-9 (MMP-9), osteoclasts were differentiated as above without DHMEQ; then DHMEQ was added after medium replacement at day 7, and the culture supernatant was collected at day 8. The concentration of MMP-9 in the supernatant was measured using an ELISA kit (GE Healthcare Bio-Sciences, Tokyo, Japan).
Suppression of in vivoosteoclastogenesis by DHMEQ
Effect of DHMEQ on production of sRANKL, osteoprotegerin and M-CSF
Suppression of NFATc1 expression by DHMEQ in arthritic joints
Suppression of human osteoclastogenesis and MMP-9 expression by DHMEQ
MMP-9 is one of the enzymes released by osteoclasts, and the enzyme plays a role in degradation of the extracellular matrix. Its expression is reported to be modulated by NFATc1 [25], and to be upregulated in serum of patients with active RA [26]. To examine the effect of DHMEQ on MMP-9 production by human osteoclasts, DHMEQ was added to the culture after formation of mature osteoclasts and secreted MMP-9 was measured. The results showed that concentration of MMP-9 in the culture supernatant was partially but significantly decreased by DHMEQ (Figure 7b). These results indicate that DHMEQ suppresses osteoclast differentiation from human peripheral blood monocytes as well as the activity of mature osteoclasts.
In the present study, we investigated the effect of DHMEQ on in vivo osteoclastogenesis using a mouse arthritis model, and showed that DHMEQ significantly suppresses differentiation of osteoclasts in arthritic joints. Serum levels of sRANKL, OPG and M-CSF, and the sRANKL/OPG ratio, were not affected by this treatment regimen with DHMEQ, whereas expression of NFATc1 in the joints was suppressed in DHMEQ-treated mice. In accordance with these observations, spontaneous expression of RANKL and M-CSF in cultures of RA-FLS were not suppressed by DHMEQ in concentrations at which it has been demonstrated to suppress expression of proinflammatory cytokines [16]. These results indicate that in a RANKL/RANK/OPG signaling cascade, expression of NFATc1, a key downstream regulator of this cascade, is more susceptible than that of upstream molecules to treatment with DHMEQ.
Expression of RANKL and OPG is coordinated to regulate bone resorption positively and negatively by controlling the activation state of RANK on osteoclasts. The crucial role of the RANKL/RANK/OPG signaling pathways in regulating bone metabolism is underscored by findings that genetic mutations that activate RANK and that inhibit the RANKL binding properties of OPG are associated with familial expansile osteolysis [27] and with juvenile Paget's disease [28], respectively. In addition, a cyclic peptide with sequence homology to a predicted ligand contact surface on RANK has been reported to inhibit RANKL-induced signaling and osteoclastogenesis [29]. Proinflammatory cytokines, such as TNFα and IL-1, are thought to modulate this system primarily by stimulating M-CSF production (thereby increasing the pool of preosteoclastic cells) and by directly increasing RANKL expression [30]. Aberrant expression of RANKL especially on RA-FLS stimulated by TNFα or IL-1 is therefore supposed to be the main contributor to bone destruction in active RA [31].
We previously demonstrated that 10 μg/ml DHMEQ suppresses expression of IL-1β and IL-6, as well as CC chemokines CCL2 and CCL5, in culture of TNFα-stimulated RA-FLS [16]. We therefore speculated that expression of M-CSF, RANKL, OPG, or the sRANKL/OPG ratio may be modulated in DHMEQ-treated mice. Serum levels of these factors, however, were not significantly affected by treatment with DHMEQ. Even in vitro, the expression of RANKL and M-CSF by RA-FLS was not enhanced by TNFα, and was not suppressed by 10 μg/ml DHMEQ. Taken together, FLS of RA patients – and presumably of mice – are suggested, once activated, to express RANKL and M-CSF rather constitutively, and they are resistant to treatment with DHMEQ. The ineffectiveness of DHMEQ on RANKL suppression may possibly be ascribed to insensitivity of the transcription mechanism of the RANKL gene to DHMEQ. Regulation of the rate of gene expression is a complex process involving several transcription factors and gene activator/repressor proteins. For example, it has been recently reported that NF-κB collaborates with other transcription factors (early growth response-2 and early growth response-3) in expression of the RANKL gene [32]. Even in molecules whose expression is demonstrated to be NF-κB dependent in a certain assay condition, therefore, the molecules' dependency on NF-κB or sensitivity to DHMEQ treatment varies among the molecules under other conditions. The second possible reason may involve the stability of RANKL once expressed on the surface of FLS. We detected RANKL by western blotting in the lysates of RA-FLS that had been cultured for a few weeks without addition of proinflammatory cytokines (Figure 5); this is consistent with the observation of other investigators [33].
Downstream of the RANKL/RANK/OPG system, a significant part of the genetic regulation of osteoclastogenesis is performed by NF-κB. The critical role of this transcription factor is underscored by the report of Franzoso and colleagues that mice lacking the p50 and p52 subunits of NF-κB develop osteopetrosis [7]. A few years later, the same group reported that expression of p50 and p52 is not required for formation of RANK-expressing osteoclast progenitors but is essential for RANK-expressing osteoclast precursors to differentiate into osteoclasts in response to RANKL and other osteoclastogenic cytokines [8]. In a rat overiectomized model of estrogen deficiency, administration of NF-κB decoy oligodeoxynucleotides attenuated the increase of TRAP activity, accompanied by a significant increase in calcium concentration in the tibia and femur [9]. A cell-permeable peptide inhibitor of the IκB kinase complex reduced the number of osteoclasts in the joints of collagen-induced arthritic mice [10].
How NF-κB is involved in osteoclastogenesis, however, had not been elucidated until Takatsuna and colleagues demonstrated that DHMEQ suppresses osteoclastogenesis by downregulation of NFATc1 in a culture system of mouse bone marrow-derived monocyte/macrophage precursor cells stimulated with RANKL and M-CSF [12]. The essential role of NFATc1 in osteoclastogenesis was also demonstrated in a recent in vivo study using osteoclast-deficient Fos-/- mice [34]. In the present study, we found that expression of NFATc1 along the inner surfaces of bone lacunae and eroded bone surface in arthritic joints is suppressed by DHMEQ, suggesting that in vivo expression of NFATc1 is significantly regulated by NF-κB in agreement with the in vitro studies. RANKL induces NFATc1 expression via three intracellular signaling pathways; an NF-κB pathway, a mitogen-activated protein kinase pathway, and a c-Fos pathway. RANKL also evokes Ca2+ oscillation, which leads to calcineurin-mediated activation of NFATc1 [13]. DHMEQ does not inhibit activation of mitogen-activated protein kinases or inhibit Ca2+ oscillation [12]; the present study therefore also indicates that the NF-κB pathway has priority over other pathways to induce NFATc1 expression.
In vivo administration of the NF-κB inhibitor DHMEQ suppressed differentiation of osteoclasts in collagen-induced mouse arthritis. In addition, DHMEQ exhibited suppressive effects on in vitro differentiation and activation of human osteoclasts, suggesting the possible clinical application of this compound.
= dehydroxymethylepoxyquinomicin
= Dulbecco's modified Eagle's medium
= enzyme-linked immunosorbent assay
= fetal calf serum
= fibroblast-like synovial cells
= interleukin
= macrophage colony stimulating factor
= matrix metalloprotease
= nuclear factor of activated T cells
= nuclear factor
= osteoprotegerin
= phosphate-buffered saline
= rheumatoid arthritis
= receptor activator of NF-κB
= receptor activator of NF-κB ligand
- sRANKL:
= soluble receptor activator of NF-κB ligand
= tumor necrosis factor
= tartrate-resistant acid phosphatase
The authors thank Fumiko Inoue and Dr Soichiro Ito (Tokyo Medical and Dental University) for their expert technical assistance.
Authors' Affiliations
- Lipsky PE, van der Heijde DM, St Clair EW, Furst DE, Breedveld FC, Kalden JR, Smolen JS, Weisman M, Emery P, Feldmann M, et al: Infliximab and methotrexate in the treatment of rheumatoid arthritis. Anti-tumor necrosis factor trial in rheumatoid arthritis with concomitant therapy study group. N Engl J Med. 2000, 343: 1594-1602. 10.1056/NEJM200011303432202.View ArticlePubMedGoogle Scholar
- van der Heijde D, Klareskog L, Rodriguez-Valverde V, Codreanu C, Bolosiu H, Melo-Gomes J, Tornero-Molina J, Wajdula J, Pedersen R, Fatenejad S: Comparison of etanercept and methotrexate, alone and combined, in the treatment of rheumatoid arthritis. Two-year clinical and radiographic results from the TEMPO study, a double-blind, randomized trial. Arthritis Rheum. 2006, 54: 1063-1074. 10.1002/art.21655.View ArticlePubMedGoogle Scholar
- Smolen JS, van der Heijde DMFM, St Clair EW, Emery P, Bathon JM, Keystone E, Maini RN, Kalden JR, Schiff M, Baker D, et al: Predictors of joint damage in patients with early rheumatoid arthritis treated with high-dose methotrexate with or without concomitant infliximab. Results from the ASPIRE trial. Arthritis Rheum. 2006, 54: 702-710. 10.1002/art.21678.View ArticlePubMedGoogle Scholar
- Redlich K, Hayer S, Ricci R, David J-P, Tohidast-Akrad M, Kollias G, Steiner G, Smolen JS, Wagner EF, Schett G: Osteoclasts are essential for TNF-α-mediated joint destruction. J Clin Invest. 2002, 110: 1419-1427. 10.1172/JCI200215582.PubMed CentralView ArticlePubMedGoogle Scholar
- Teitelbaum SL: Bone resorption by osteoclasts. Science. 2000, 289: 1504-1508. 10.1126/science.289.5484.1504.View ArticlePubMedGoogle Scholar
- Boyle WJ, Simonet WS, Lacey DL: Osteoclast differentiation and activation. Nature. 2003, 423: 337-342. 10.1038/nature01658.View ArticlePubMedGoogle Scholar
- Franzoso G, Carlson L, Xing L, Poljak L, Shores EW, Brown KD, Leonardi A, Tran T, Boyce BF, Siebenlist U: Requirement for NF-κB in osteoclast and B-cell development. Genes Dev. 1997, 11: 3482-3496.PubMed CentralView ArticlePubMedGoogle Scholar
- Xing L, Bushnell TP, Carlson L, Tai Z, Tondravi M, Siebenlist U, Young F, Boyce BF: NF-κB p50 and p52 expression is not required for RANK-expressing osteoclast progenitor formation but is essential for RANK- and cytokine-mediated osteoclastogenesis. J Bone Miner Res. 2002, 17: 1200-1210. 10.1359/jbmr.2002.17.7.1200.View ArticlePubMedGoogle Scholar
- Shimizu H, Nakagami H, Tsukamoto I, Morita S, Kunugiza Y, Tomita T, Yoshikawa H, Kaneda Y, Ogihara T, Morishita R: NF-κB decoy oligodeoxynucleotides ameliorates osteoporosis through inhibition of activation and differentiation of osteoclasts. Gene Ther. 2006, 13: 933-941. 10.1038/sj.gt.3302711.View ArticlePubMedGoogle Scholar
- Jimi E, Aoki K, Saito H, D'Acquisto F, May MJ, Nakamura I, Sudo T, Kojima T, Okamoto F, Fukushima H, et al: Selective inhibition of NF-κB blocks osteoclastogenesis and prevents inflammatory bone destruction in vivo. Nat Med. 2004, 10: 617-624. 10.1038/nm1054.View ArticlePubMedGoogle Scholar
- Abu-Amer Y, Dowdy SF, Ross FP, Clohisy JC, Teitelbaum SL: TAT fusion proteins containing tyrosine 42-deleted IκBα arrest osteoclastogenesis. J Biol Chem. 2001, 276: 30499-30503. 10.1074/jbc.M104725200.View ArticlePubMedGoogle Scholar
- Takatsuna H, Asagiri M, Kubota T, Oka K, Osada T, Sugiyama C, Saito H, Aoki K, Ohya K, Takayanagi H, Umezawa K: Inhibition of RANKL-induced osteoclastogenesis by (-)-DHMEQ, a novel NF-κB inhibitor, through downregulation of NFATc1. J Bone Miner Res. 2005, 20: 653-662. 10.1359/JBMR.041213.View ArticlePubMedGoogle Scholar
- Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, Saiura A, Isobe M, Yokochi T, Inoue J, et al: Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell. 2002, 3: 889-901. 10.1016/S1534-5807(02)00369-6.View ArticlePubMedGoogle Scholar
- Ariga A, Namekawa J, Matsumoto N, Inoue J, Umezawa K: Inhibition of tumor necrosis factor-α-induced nuclear translocation and activation of NF-κB by dehydroxymethylepoxyquinomicin. J Biol Chem. 2002, 277: 24625-24630. 10.1074/jbc.M112063200.View ArticlePubMedGoogle Scholar
- Miyajima A, Kosaka T, Seta K, Asano T, Umezawa K, Hayakawa M: Novel nuclear factor κB activation inhibitor prevents inflammatory injury in unilateral ureteral obstruction. J Urol. 2003, 169: 1559-1563. 10.1097/01.ju.0000045686.21766.c1.View ArticlePubMedGoogle Scholar
- Wakamatsu K, Nanki T, Miyasaka N, Umezawa K, Kubota T: Effect of a small molecule inhibitor of nuclear factor-κB nuclear translocation in a murine model of arthritis and cultured human synovial cells. Arthritis Res Ther. 2005, 7: R1348-R1359. 10.1186/ar1834.PubMed CentralView ArticlePubMedGoogle Scholar
- Chiba T, Kondo Y, Shinozaki S, Kaneko E, Ishigami A, Maruyama N, Umezawa K, Shimokado K: A selective NFκB inhibitor, DHMEQ, reduced atherosclerosis in apoE-deficient mice. J Atheroscler Thromb. 2006, 13: 308-313.View ArticlePubMedGoogle Scholar
- Lee S-K, Lorenzo J: Cytokines regulating osteoclast formation and function. Curr Opin Rheumatol. 2006, 18: 411-418.View ArticlePubMedGoogle Scholar
- Suzuki Y, Sugiyama C, Ohno O, Umezawa K: Preparation and biological activities of optically active dehydroxymethylepoxyquinomicin, a novel NF-κB inhibitor. Tetrahedron. 2004, 60: 7061-7066. 10.1016/j.tet.2004.01.103.View ArticleGoogle Scholar
- Tadokoro M, Hattori K, Takakura Y, Ohgushi H: Rapid preparation of fresh frozen tissue-engineered bone sections for histological, histomorphological and histochemical analyses. Biomed Mater Eng. 2006, 16: 405-413.PubMedGoogle Scholar
- Arnett FC, Edworthy SM, Bloch DA, Mcshane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR, Liang MH, Luthra HS, et al: The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988, 31: 315-324. 10.1002/art.1780310302.View ArticlePubMedGoogle Scholar
- Nanki T, Nagasaka K, Hayashida K, Saita Y, Miyasaka N: Chemokines regulate IL-6 and IL-8 production by fibroblast-like synoviocytes from patients with rheumatoid arthritis. J Immunol. 2001, 167: 5381-5385.View ArticlePubMedGoogle Scholar
- Geusens PP, Landewé RBM, Garnero P, Chen D, Dunstan CR, Lems WF, Stinissen P, van der Heijde DMFM, van der Linden S, Boers M: The ratio of circulating osteoprotegerin to RANKL in early rheumatoid arthritis predicts later joint destruction. Arthritis Rheum. 2006, 54: 1772-1777. 10.1002/art.21896.View ArticlePubMedGoogle Scholar
- Komano Y, Nanki T, Hayashida K, Taniguchi K, Miyasaka N: Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts. Arthritis Res Ther. 2006, 8: R152-10.1186/ar2046.PubMed CentralView ArticlePubMedGoogle Scholar
- Sundaram K, Nishimura R, Senn J, Youssef RF, London SD, Reddy SV: RANK ligand signaling modulates the matrix metalloproteinase-9 gene expression during osteoclast differentiation. Exp Cell Res. 2007, 313: 168-178. 10.1016/j.yexcr.2006.10.001.View ArticlePubMedGoogle Scholar
- Fiedorczyk M, Klimiuk PA, Sierakowski S, Gindzienska-Sieskiewicz E, Chwiecko J: Serum matrix metalloproteinases and tissue inhibitors of metalloproteinases in patients with early rheumatoid arthritis. J Rheumatol. 2006, 33: 1523-1529.PubMedGoogle Scholar
- Hughes AE, Ralston SH, Marken J, Bell C, MacPherson H, Wallace RGH, van Hul W, Whyte MP, Nakatsuka K, Hovy L, Anderson DM: Mutations in TNFRSF11A, affecting the signal peptide of RANK, cause familial expansile osteolysis. Nat Genet. 2000, 24: 45-48. 10.1038/71667.View ArticlePubMedGoogle Scholar
- Whyte MP, Obrecht SE, Finnegan PM, Jones JL, Podgornik MN, McAlister WH, Mumm S: Osteoprotegerin deficiency and juvenile Paget's disease. N Engl J Med. 2002, 347: 175-184. 10.1056/NEJMoa013096.View ArticlePubMedGoogle Scholar
- Aoki K, Saito H, Itzstein C, Ishiguro M, Shibata T, Blanque R, Mian AH, Takahashi M, Suzuki Y, Yoshimatsu M, et al: A TNF receptor loop peptide mimic blocks RANK ligand-induced signaling, bone resorption, and bone loss. J Clin Invest. 2006, 116: 1525-1534. 10.1172/JCI22513.PubMed CentralView ArticlePubMedGoogle Scholar
- Khosla S: The OPG/RANKL/RANK system. Endocrinology. 2001, 142: 5050-5055. 10.1210/en.142.12.5050.View ArticlePubMedGoogle Scholar
- Takayanagi H: Mechanistic insight into osteoclast differentiation in osteoimmunology. J Mol Med. 2005, 83: 170-179. 10.1007/s00109-004-0612-6.View ArticlePubMedGoogle Scholar
- Fionda C, Nappi F, Piccoli M, Frati L, Santoni A, Cippitelli M: 15-Deoxy-Δ12,14-prostaglandin J2negatively regulates rankl gene expression in activated T lymphocytes: role of NF-κB and early growth response transcription factors. J Immunol. 2007, 178: 4039-4050.View ArticlePubMedGoogle Scholar
- Lee CK, Lee EY, Chung SM, Mun SH, Yoo B, Moon HB: Effects of disease-modifying antirheumatic drugs and antiinflammatory cytokines on human osteoclastogenesis through interaction with receptor activator of nuclear factor κB, osteoprotegerin, and receptor activator of nuclear factor κB ligand. Arthritis Rheum. 2004, 50: 3831-3843. 10.1002/art.20637.View ArticlePubMedGoogle Scholar
- Asagiri M, Sato K, Usami T, Ochi S, Nishina H, Yoshida H, Morita I, Wagner EF, Mak TW, Serfling E, Takayanagi H: Autoamplification of NFATc1 expression determines its essential role in bone homeostasis. J Exp Med. 2005, 202: 1261-1269. 10.1084/jem.20051150.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text:
Lorell Leather Cube ChairReception, Side & Guest Chairs
18" x 18" x 18" - Leather - Black - View Complete Details
Product Number: LLR35854
In Stock - Hurry, only 162 left.
Estimated Delivery - 1 business day(s) - Mon 2/27 2 business days - Tue 2/28
Free Returns - Return this item free of charge. View Details.
Free Shipping - This item qualifies for Free Shipping
- Add to Compare
- Request Quote
- Read Questions & Answers
You May Also Like
Product Details
Lorell Leather Cube Chair - As part of the Lorell Collaborative Seating collection, this inviting cube ottoman helps you create opportunities for casual seating in public areas. Arrange several of these modular lounge pieces together. This leather-upholstered seat can also be used as a side table or foot stool. Durable leather is both attractive and practical for high-traffic areas. Design also includes a sturdy plywood frame and adjustable leveling glides on feet.View Product Specs
Still have a question? Click to ask a Question
ReviewsThere are no Customer Reviews yet.
Help out our customers.
Be the first to write a review.
Question & Answer
No questions have been posted for this product.
Have a question about this product? Please click the button below to get started. Once you have posted your question we will submit it to our community of customers and with our own staff who have recent experience with the same product for their open and honest feedback.Ask a Question


text:
News By Tag
Industry News
News By Place
Industry News
Path to Unity of Consciousness: The Episode of Life Published by Outskirts Press
Outskirts Press announces the latest highly anticipated philosophy and religion book from Marietta, GA, author Amjad Fatemi, Ph.D.
Traditionally, the back cover of a book contains some description of the author's credits, perhaps a short biography, and the author's picture. Below those are endorsements by some distinguished figure or authority in the book's subject. This process has been, and will be.
But this book steps away from this tradition and the norm of publication and, in fact, is challenging any kind of tradition and authority or fame as egoistic aspects of writer -- instead giving authority to the reader and the reader's endorsement. The reader's endorsement is important as a part of the unity of consciousness. There is no difference between "you" and some "recognized authority" upon any subject.
All readers need to do is to think and to try to find a connection between their inner selves and the external knowledge. This is what the book is about.
564 pages in length, The Episode of Life: Consciousness, Creation, and the Universe is being aggressively promoted to appropriate markets with a focus on the philosophy and religion category. With U.S. wholesale distribution through Ingram and Baker & Taylor, and pervasive online availability through Amazon, Barnes & Noble and elsewhere, The Episode of Life meets consumer demand through both retail and library markets with a suggested retail price of $26.95.
Additionally, The Episode of Life can be ordered by retailers or wholesalers for the maximum trade discount price set by the author in quantities of ten or more from the Outskirts Press Direct bookstore at www.outskirtspress.com/
ISBN: 9781432769628 Format:
For more information or to contact the author, visit www.outskirtspress.com/
About the author:
Amjad Fatemi was born in Persia and graduated from London University with a Ph.D. in chemistry. During the last thirty-five years he has been involved teaching Industrial Research and Development in the university, lecturing and studying. He has published two other books in his native language: Key to the Supreme World and Passage to Beyond of Thought. For the last twenty-five years his focus -- beside mundane life and supporting family -- was upon on this writing. He has given more than one hundred lectures about the evolution of consciousness in public. He is married has three children and is living in the U.S.A.
About Outskirts Press, Inc.
Outskirts Press, Inc. offers full-service, custom self-publishing and book marketing services for authors seeking a cost-effective, fast, and flexible way to publish and distribute their books worldwide while retaining all their rights and full creative control. Available for authors globally at www.outskirtspress.com and located on the outskirts of Denver, Colorado, Outskirts Press represents the future of book publishing, today.
Account Email Address Account Phone Number Disclaimer Report Abuse


text:
Oakland Summer Leadership Academy
NEW Richmond Office!
August 3, 6:30pm-8pm. We invite you to join us at our NEW office in Richmond!
Come for the refreshments, stay for the conversation, and get plugged into taking action for environmental justice. Click here for Event details and please RSVP.
RSVP by August 1 to: email@example.com or 323-826-9771 x113
Our new location: 120 Broadway, Suite 2, Richmond CA 94804
Door prize: Made in Richmond gift basket from CA Delicious
Latest Press Release 6/14/2016
Cap Emissions Now Press Conference
Air District regulation can protect public health and climate by blocking Big Oil's push to bring in dirtier crudes (PDF)
Join CBE at South East LA Ciclavia this Sunday!
Celebrate our clean energy future and test drive a brand new EV.
Join CBE This Saturday! 1PM at Los Angeles City Hall
Toxic Tour/ Tur Toxico
Saturday/ Sabdao, 4/23/2016 10am-2pm: Celebrate Mother Earth by learning about environmental racism in Southeast Los Angeles. Celebre a la Madre Tierra en el Sureste de Los Ángeles y aprende sobre el justicia ambiental. More information here / Mas información aqui .
Reserve by/ Reservar antes del April 15, 2016:
Milton Nimatju at nimatuj[at]cbecal[dot]org or call 323-826-9771×112
Green Fair and Electric Vehicle Test Drive
Sat., January 30, 2016 | 11am-2pm | 325 N Neptune Ave. Wilmington, CA 90744. Find out how you can go green to reduce pollution. Maneje autos electricos y feria de recursos verde. Click for English Flyer or Información en español
POSTPONED. Wilmington Public Toxic Tour
December 5, 10am-2pm | RSVP to learn about our recent and past victories in Wilmington, community of Los Angeles. You'll hear directly from CBE community members pushing back against toxic polluters and get plugged into taking action.
Youth for Environmental Justice 18th Birthday
December 12, 6pm-12am | 4063 Gage Ave, Bell CA 90201. We invite you to celebrate 18 years of youth organizing! For more information and to REGISTER visit: http://bit.ly/YouthEJ18


text: Apple Recalling Verizon iPad 2s?Sam Biddle6/10/11 11:00amFiled to: Appleipad 2ipadVerizonVerizon ipadRecall34EditPromoteShare to KinjaToggle Conversation toolsGo to permalinkRuh roh. 9to5Mac reports Apple's checking certain Verizon iPad 2 serial numbers, and sending offending units back to China mid-transit. SAI says "The iPads are having problems viewing and editing account information after activating with Verizon's network." Is yours delayed? Advertisement The problems would jibe with earlier reports of 3G connectivity issues—but if the 'Pads are being sent back to the plant instead of waiting on a software fix, the problem could be worse than originally believed.If you've noticed your Verizon iPad 2 being turned around on the FedEx train, let us know.[9to5Mac]Reply34 repliesLeave a reply


text:
- Price: $85.00
- Exclusive Price: $3500.00
Globex Business After Effects Intro 39674
After Effects Intro that was composed by our webmaster artist Corvus.
Sources, Software & Requirements
Globex Business After Effects Intro 39674 is available in the following sources: .AEP. Software required includes Winzip 9+ (Windows) or Stuffit Expander 10+ (Mac) for uncompressing .zip files, Adobe After Effects CS4, No additional plugins required, and Adobe After Effects CS5. It has had a total of total of 2 downloads since it first appeared.


text:
The Lilly Ledbetter Fair Pay Restoration Act is the first bill President Obama signed
The Lilly Ledbetter Fair Pay Restoration Act, named for a former Goodyear Tire employee who sued the company for gender discrimination in 1998, is the first bill signed by Obama.
"It is fitting that with the very first bill I sign ... we are upholding one of this nation's first principles: that we are all created equal and each deserve a chance to pursue our own version of happiness," Obama said at a ceremony in the East Room of the White House.
"If we stay focused, as Lilly did, and keep standing for what's right, as Lilly did, we will close that pay gap and ensure that our daughters have the same rights, the same chances, and the same freedom to pursue their dreams as our sons."The new law removes a provision requiring employees seeking equal pay to file a complaint within 180 days of receiving their first unfair paycheck.
Under the measure, employees instead have the right to file within 180 days of their most recent paycheck.
Supporters of the Ledbetter Act have argued that, under the old standard, an employer merely needed to hide unfair pay practices for three months before being able to continue them without penalty forever.
Lilly Ledbetter was awarded $360,000 in back pay by a federal judge in Alabama, but the verdict was overturned in a 5-4 Supreme Court ruling in May 2007.
The court said that even though she filed her complaint within 180 days of when she first learned that she was getting paid less than comparable male employees, she had failed to file within 180 days of the first unequal paycheck.
During the 2008 presidential campaign, the Ledbetter Act proved to be a significant point of contention between Obama and Republican nominee Sen. John McCain. Obama heavily emphasized what he called the plan's benefits to working women, while McCain criticized it as a boon for trial lawyers.
Obama later danced with Ledbetter at one of his inaugural balls. She also spoke at the 2008 Democratic National Convention."My case is over. I will never receive the pay I deserve," Ledbetter said in that speech. "But there will be a far richer reward if we secure fair pay for our children and grandchildren, so that no one will ever again experience the discrimination that I did."


text:
A pattern common to families that suffer domestic abuse is that the abused child grows up to become an adult who reenacts the abuse he received in his youth. Sometimes it's only a matter of time before self-destructive behaviors which have lain dormant (or which the abused child hopes to have conquered) resurface to destroy years of hard work aimed at overcoming one's past.
The Berkeley Repertory Theatre recently presented Black N Blue Boys/Broken Men as part of a world premiere co-production with the Goodman Theatre in Chicago. A deeply disturbing one-woman show written and performed by Dael Orlandersmith, this powerful monologue delivers 90 minutes of gut-wrenching tales about young boys who, after being abused by their fathers, mothers, uncles, and friends of the family, grew into adults sitting on a powder keg of repressed anger.
Dael Orlandersmith is the writer and solo performer of Black N Blue Boys/Broken Men
Among the battered souls brought to life by Orlandersmith are:
- Flaco, a young man whose mother's roving fingers would not leave him alone. No one believes Flaco when he complains about his mother's insistence on reaching into his pants and fondling his dick, so he finally gives up and starts having sex with her. After his father walks into the room one afternoon and finds Flaco fucking his mother, the boy is thrown out of the apartment and, at the age of 12, learns from a friend how to hustle tricks under the Coney Island boardwalk. A pimp gives Flaco $30 each time he satisfies a customer but, upon learning that the pimp is charging his customers $300, Flaco gets mad, leaves the shelter of the pimp's home, and starts turning tricks in the West Village. Often, his clients are wealthy businessmen who want a threeway with their wives or girlfriends. One night, Flaco is picked up by a man in a limousine who fucks the 14-year-old boy until he bleeds, then throws $400 in his face and orders him to get the hell out of the car. That $400 is the most money Flaco has ever made in one day, but it makes him feel awful. He can't wait for a chance to wash himself off.
- Tenny, the uncle everyone loves and trusts. One day, after Tenny picked his nephew up from swimming practice, the boy begged him for some ice cream. After a stop at Dairy Queen, they got back in the car with their ice cream and the young boy innocently licked Tenny's face to get a taste of Tenny's vanilla ice cream. Tenny returned the favor, but soon started licking more and more of his nephew's young body until he had pulled the kid's pants down and raped him.
Dael Orlandersmith (Photo by: Kevin Berne)
- Ian grew up in one of Britain's council houses (the equivalent of an American housing project). His father was a violent drunk who always came home from the pub in a dangerous state. As Ian matured, he was the only one of his friends who never touched alcohol. Finally, he got up the courage to leave home, moved to London, and got a job in a nice restaurant. Upon moving to New York, he continued to work at bettering himself and eventually started dating an attractive woman from Greenwich, Connecticut. On the night she threw a birthday party for him in his own apartment, Ian had two glasses of wine. He was fucking a stranger in the bathroom when his girlfriend walked in on them. Like his father, he erupted and became a violent, sadistic drunk.
- Mikey fell in love with books at an early age and spent long hours at the library to avoid the toxic atmosphere at home. Books became his safety net, taking him to faraway places and on grand adventures as he put himself through college and eventually got a job working with runaway children. One night, his assistant didn't show up at the shelter where he worked, so he had to cook dinner for the kids (one of whom kept calling him "98 cent Mike" and teasing Mike because he was a "trick baby"). Suddenly, Mike snapped, spanked the living daylights out of the kid, and barely even knew what had come over him.
- Another young boy grew up watching his mother and her friends take turns going into the bathroom where they should shoot up before reemerging in a very sleepy state. Occasionally his mother would get better, but soon her new boyfriend started shooting up as well. The boy tried to protect his baby sister but, one day, the infant saw what her mother was doing and tried to mimic her behavior. In the process, the young girl ate the contents of a small bag of white powder and died of an overdose. Even today, the older brother feels guilty for the girl's death and misses her terribly.
Dael Orlandersmith in a moment from her monologue entitled
Black N Blue Boys/Broken Men (Photo by: Kevin Berne)
Orlandersmith's writing is powerful, beautifully edited, and goes to very dark places that some theatregoers may not want to visit with her. A former social worker who once was employed at a shelter for runaway children, she paints devastating portraits of children whose youth and innocence were stolen from them by irresponsible adults.
Chay Yew, who directed the piece with acute sensitivity to pacing, was helped tremendously by Ben Stanton's subtle lighting and Mikhail Fiksel's sound design. Unlike many monologues (which are performed on rather shallow stages), Daniel Ostling's angular unit set reached far upstage into the heart of darkness, despair, depravity, and degradation in which these children were raised.
Black N Blue Boys/Broken Men is a riveting evening anchored by Orlandersmith's hypnotic power as a storyteller. Here's the trailer:
To read more of George Heymont go to My Cultural Landscape
Every Friday, HuffPost's Culture Shift newsletter helps you figure out which books you should read, art you should check out, movies you should watch and music should listen to. Learn more


text: On Thu, Jun 15, 2006 at 03:41:20PM -0600, Steven Mosimann wrote: > (1) Replaced the failed disk with one of the same size > (2) Used pvcreate to make the same physical volumes on the new disk > that were present on the failed disk. Example of the actual command I > used was: > pvcreate --uuid xxxxx /dev/sdb1 > where xxxxx was the pv uuid from pvdisplay Missing the --restorefile arg there (for safety - if it was originally created by lvm2 with default options it'll make no difference). Missing a 'vgcfgrestore' step. > (3) Used vgchange -a y -P to activate the volume group No need for -P here. That's what you use if you *don't* do steps 1 and 2 etc. Alasdair -- agk redhat com


text:
News By Tag
* Buycheap digital cameras
* Latest Digital Cameras
* Online Electronic Store
* Electronic Bazaar
* More Tags...
Industry News
News By Location
Industry News
Guide To Buy Cheap Digital Cameras
Latest digital cameras have become popular worldwide because of their high-end features and a better picture clarity. Below, we have discussed some prominent steps to help one buy cheap digital cameras with ease.
If you are looking forward to buy cheap digital cameras with advanced features, this article can be of great help. Some prominent factors that can help you choose a gadget as per your requirements and budget include:
Know your requirements
First of all, you need to know your purpose behind the purchase. What kind of camera you require? What features you wish to see in your device? These are some of the basic things you need to know before making a purchase.
Go online
This is one of the best ways of finding a gadget of your choice at an affordable rate. Be it a mobile phone, a laptop, a camcorder or a digital camera, you can consider buying it online. The medium offers an array of options to the customers and that too at affordable rates. This is because a majority of suppliers today offer their products online. The process is beneficial for the buyer as well as seller. You first need to check the websites and products of different suppliers.
Compare the products
This is another important step one needs to follow. Choosing a website is not enough. You need to go through all its products, features and pricing details. Always compare the features and prices of products available from different brands. This will definitely help you choose the latest digital cameras at pocket-friendly rates.
Go through reviews
Always go through the reviews available on website posted by other individuals who have already used the products. These reviews prove very helpful as you come to know about the views of different users regarding that particular product.
Check-out deals
Most of the suppliers keep on announcing deals and offers on their products from time to time. These deals and offers can help you buy cheap digital cameras of your choice.
These are some of the important factors that can help you make a fair decision regarding the purchase of your favourite gadget.
Electronic Bazaar is the best place to buy cheap digital cameras equipped with user-friendly features and latest technology. All the latest digital cameras available at the online electronic store are reasonably-priced.
Contact Us:
Electronic Bazaar
PO Box 6110 ST KILDA RD CENTRAL VIC 8008
Email :firstname.lastname@example.com
Customer Care No. : +61 3 9016 3949
Website: www.electronicbazaar.com.au
Account Email Address Account Phone Number Disclaimer Report Abuse


text:
The Oxalis clover grows from a bulb. It only grows here during the cooler
months, then disapears. There are a number of different ones, different
shapes, sizes, bloom colors. One of my all time favorite, although not a "weed"
is the purple Oxalis....that particular one does stick around all year long,
and isn't nearly as prolific, but does clump nicely.
zone 9
Texas Gulf Coast
In a message dated 5/19/2008 6:47:32 PM Central Daylight Time,
email@example.com writes:
sounds like the red clover we have here, but it is with us all summer
**************Wondering what's for Dinner Tonight? Get new twists on family
favorites at AOL Food.
To sign-off this list, send email to email@example.com with the
message text UNSUBSCRIBE GARDENCHAT


text:
Browse other items in the Kodiak Monterey collection from Wayside Furniture in the Akron, Cleveland, Canton, Medina, Youngstown, Ohio area.
The Monterey Futon is the perfect addition to your home. Made with hardwood, this piece is sure to be strong and long-lasting. A dorm room, game room or den staple, the futon can be used as a sofa when in the upright position or laid flat as a space to sleep. This futon features a unique style arm, offering a casual look. Not only is this piece functional, but it is also versatile, as it can be used in a dorm room, guest bedroom, or cabin.Available in six wood finishes and approximately 30 fabric choices. Price will range from $499 to $599 depending on fabric.Under-bed storage available in matching six wood finishes $149.99.
The Kodiak Monterey Monterey Futon by Wayside Furniture from Wayside Furniture. We proudly serve the Akron, Cleveland, Canton, Medina, Youngstown, Ohio area. Product availability may vary. Contact us for the most current availability on this product.
This collection of wood futons provides you with plenty of seating options for your home. Whether you're looking to furnish your cabin, guest room, or dorm room, these pieces are sure to please. Use the piece up as a sofa, or lay it flat for guests to sleep on. These futons are available in multiple finishes and provide beautiful and functional features, such as lift-up trays, loungers, and more! For a stylish, useful, and quality home, look no further than this collection of futons.
The Kodiak Monterey collection is a great option if you are looking for Casual furniture in the Akron, Cleveland, Canton, Medina, Youngstown, Ohio area.
This item has been tagged as: Sleeper Sofa, Sleeper Couch, Sofa with a Hide-A-Bed, Queen Sleeper Sofa, Full Size Sleeper Sofa, Sofa Bed, Love Seat Sleeper, Hide-A-Bed, Futon, Futon Sofa Sleepers, Convertible Sofa, Couch with Sleeper, Pull – Out Sofa, Couch That Becomes a Bed, Sofa Beds, Futon That Opens Into A Bed
Nothing on this page may be copied or reproduced without explicit permission.
Drag anItem Here
To start a new blank room plan, enter the desired room dimensions below and click Create Room.
To request a new password, enter your username or e-mail address and click submit. A new password will automatically be emailed to you.
If you are still having trouble logging on after receiving your new password, please email firstname.lastname@example.com


text: Events Search Events Filter by: Industry Team Airport & AviationAutomotiveEnergyFood & Beverage Health CareInsurance & ReinsuranceLife SciencesManufacturing SportsTechnology Practice Area Business Law All Business LawEmployee Benefits & Executive CompensationEnvironmental RegulationEstates & TrustsFinance & Financial Institutions Private Equity & Venture CapitalReal EstateSecurities, Commodities & Exchange RegulationTaxationTransactional & Securities Government & Public Policy All Government & Public PolicyEPA RegulatoryExport Controls & National SecurityFDA RegulatoryFederal & State Energy Regulatory Freight & Commuter RailGovernment ProcurementPolitical LawPublic Policy Intellectual Property All Intellectual PropertyBusiness Method & Software PatentsChemical, Biotechnology & PharmaceuticalCybersecurityElectronicsGreen Energy TechnologiesIP Asset Management IP Due DiligenceIP LitigationMechanical & Electromechanical TechnologiesPatent Office TrialsTechnology Transactions & OutsourcingTrademark, Copyright & Advertising Counseling International All InternationalChinaEurope JapanLatin America Litigation All LitigationAntitrustAppellateBankruptcy & Business ReorganizationsBusiness Litigation & Dispute ResolutionConsumer Financial ServicesDistribution & Franchise Government Enforcement, Compliance & White Collar DefenseInsurance & Reinsurance LitigationLabor & EmploymentPrivacy, Security & Information ManagementSecurities Enforcement & LitigationTrade Secret Noncompete Litigation Office BostonBrusselsChicagoDetroitJacksonvilleLos AngelesMadison MiamiMilwaukeeNew YorkOrlandoSan DiegoSan FranciscoShanghai Silicon ValleyTallahasseeTampaTokyoWashington, D.C. Series Access to Capital Web Conference SeriesAnnual Update on Government ContractsAvanzando: Bridge to the Future of Latin American BusinessCheckpoint Web Conference SeriesConsumer Finance Web Conference Series Employee Benefits BroadcastEnergy Bootcamp Web Conference SeriesFoley Executive Briefing SeriesHealth Information Technology Web Conference SeriesLabor & Employment Breakfast Briefing Series – Tending to Your Business Labor & Employment Inner Workings Web Conference SeriesNewsFeed Food Industry Web Conference SeriesPediatric Business and Healthcare Delivery Series Featured All Filters applied: Search phrase entered: Sort results by: Most Recent Most Viewed Most Shared Event Series Pages: Access to Capital Web Conference Series Annual Update on Government Contracts Avanzando: Bridge to the Future of Latin American Business Checkpoint Web Conference Series Consumer Finance Web Conference Series Employee Benefits Broadcast Energy Bootcamp Web Conference Series Foley Executive Briefing Series Friday Focus Health Care Web Conference Series Health Information Technology Web Conference Series Insurance Industry Connection Web Conference Series Labor & Employment Breakfast Briefing Series – Tending to Your Business Labor & Employment Inner Workings Web Conference Series NewsFeed Food Industry Web Conference Series Pediatric Business and Healthcare Delivery Series Timely Insight: The Hospitality Industry Web Conference Series Upcoming Past Register Now The Rising Storm: Private Equity and the Foreign Corrupt Practices Act Mar 07 07 March 2012 –Boston, MA In February 2011, The Wall Street Journal reported that the SEC had launched a wide-ranging inquiry into the private equity industry, including looking into "higher-risk"... Register Now Avoiding and Resolving International Business Disputes Feb 01 01 February 2013 –Boston, MA International business leads inevitably to international disputes. Strategies for avoiding and resolving such disputes are more varied and complex than ever. Related 1 2 3


text:
Absolutely pristine and beautiful chrome RonJon 19 x 8.5 wheels. These wheels are perfect. There is zero road rash. I will let the pictures speak for themselves. These rims cost retail for $435 each. Brand new chrome centercaps. That's $1740 before taxes. $1000 OBO. No low ballers and I will not accept any form of payment except cash and money order. Shipping to the lower 48 will be what it cost me. Make me an offer.Diameter: 19 inchesWidth: 8.5 inchesLug pattern: 5x114.3Offset: +42Condition of tires: Not included
RonJon Inspyre
Part number: RJ398551C
Bolt Pattern: 5x114.3
Diameter: 19"
Width: 8.5"
Offset: +42
Center Bore: 64.1
Brilliant Chrome
RonJon Sport Design


text:
I humbly salute you, O you faithful,
heavenly Friends of my children!
I give you heartfelt thanks
for all the love and goodness you show them.
At some future day I shall,
with thanks more worthy than I can now give,
repay your care for them,
and before the whole heavenly court
acknowledge their indebtedness to your guidance and protection.
Continue to watch over them.
Provide for all their needs of body and soul.
Pray, likewise, for me,
for my husband,
and my whole family,
that we may all one day rejoice in your blessed company.
Four things to know about your candle on Catholic Online:
Eternal Father, I desire to rest in Thy Heart this night. I make the intention of offering to Thee every beat of my heart, joining to them as many acts of love and desire. I pray that even while I am asleep, I will bring back to Thee souls that offend Thee. I ask forgiveness for the whole ... continue readingMore Prayer of the Day
In the centuries old Catholic tradition, holy cards or prayer cards are small, devotional cards for the use of the faithful. Prayer cards have an endless amount of use and make wonderful gifts and keepsakes.


text:
GIVE TO CEC ARTS, is our first crowd-funding campaign. Over the course of this campaign we will bring you some stories of our past, share with you current events that you won't want to miss and look forward to our future!
We are asking for your financial and moral support to keep the center going. These are challenging times on many levels and it is more important than ever that we work to support the arts and the CEC as an important arts institution. Click here to donate.
If you are new to the Center, this short video will give you an idea of the kind of exciting events that you can become part of in the future. We hope to see you soon!
You can find a schedule of our excellent courses here.
If you are interested in teaching or performing at CEC, you can find a variety of resources here which will help your event run smoothly, from booking a space to publicizing on this website.


text:
Need a Varicella Titer in River Grove, IL? Simply order online 24/7 and get a fast, affordable Varicella Titer at a lab near you (Find More Locations):
Why would I get a Varicella Titer?
The Varicella Titer is a blood test used to prove immunity to Varicella. The Varicella Titer is frequently needed by schools, jobs, and vendor credentialing organizations in River Grove, IL.
How much does a Varicella Titer cost?
Our Varicella Titer price includes the doctor's order that you need to get tested in River Grove, IL, all lab fees and taxes, and a pdf copy of your results. For current prices, please CLICK HERE.
What does a Varicella Titer test report look like?
For a Varicella Titer sample report, please CLICK HERE.
We are delighted to offer the Varicella Titer in River Grove, IL.
Varicella Titer in other cities near River Grove, IL:Amf Ohare | Bellwood | Broadview | Chicago | Elmwood Park | Forest Park | Franklin Park | Harwood Heights | Hines | Maywood | Melrose Park | Oak Park | River Forest | Schiller Park | Stone Park


text:
Young's Knows the Value of Convenience
Browse our product catalogs, place an order online, or shop in an Express Store near you. New Customer?
Best in the West
Founded in 1888, Young's Market Company is the premier distributor of fine wine and spirits in the western United States. We are proud to represent industry-leading brands and supplier partners, supported by our Best in Class team of wine and spirits professionals.
Young's Market Company is the industry leader in wholesale and distribution of wines, spirits, and select beverages in the western United States.
If you only had one wine for Thanksgiving…
Thanksgiving is here, and I can already taste turkey and gravy! Of course, turkey is never the only menu item. At my house, there are at least a dozen other offerings, from prime rib to sweet potatoes.
Young's Family Spotlight – Latino American Partner Network
At Young's, our family is diverse and our culture is strong because of our employee network groups.
Young's Family Spotlight: Women's Partner Network Group
Our culture at Young's is strengthened by the leadership of our Women's Partner Network Group.


text:
ASURE Chelsea Gateway Motor Lodge
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Suites
- Free High-Speed Internet
- Room service
- Wheelchair accessibility
- Kitchenette
- Free parking
Photos of ASURE Chelsea Gateway Motor Lodge
Travelers also looked at:
- Free High-Speed Internet
- Wheelchair accessibility
- Kitchenette
- Free parking
- Suites
- Room service
TripAdvisor Reviews ASURE Chelsea Gateway Motor Lodge Westport
Travel Blogs from Westport
Continuing journey south
Yesterday we woke up for sunrise over the sea so that we could join the road nice and early as we had a long drive ahead. We journeyed south for 532km, leaving the national park and driving across the country to drive down the west coast. The drive was phenomenal. In no other country could I drive for so long and enjoy the entire thing. The meandering roads uphill can be a test for our engine, but the views on reward to meeting the crest of the hills ...
West coast cruising
... took us over to Westport by late afternoon. Though I had very much planned to find out a few more things from the Cherry Booths before arriving in their once upon a time hometown, I took it upon myself to do a mini tour. The town is quite small, the centre is only one long street with large municipal building, memorial park and shops. Having parked up next to the park (Victoria Square was the name I think), a relaxing walk round the ...
Off to Westport
Result! Woke up this morning and it wasn't raining! So me, Mike, Dave and Sez walked down to the beach to play a bit of footy. The sand was literally golden in colour. Best way to start the day! By 10am we were back on the bus to head to Westport. Our next destination on the Kiwi Experience. En route we stopped off at Nelson Lake. It was of course raining by now but that did not stop us getting a few pics by the lake. Even with all the clouds in the sky the view was amazing. ...
Snow is here
... in Westport. We decided we wouldn't take part in any jet boating or horse trekking as the weather was poor and we had something lined up further down the line. We finally arrived into Westport around 4 o'clock. Westport apparently has a mild climate boasting 2,000 hours of sun, more than Auckland and Christchurch, however we didn't see any, not one bit. It was cloudy and bitterly cold, but we had to remember that this is their Autumn. After ...
Hobart to Queenstown
... house. Following WW11 many foreign workers and their families from Europe came to work on the scheme and creating in time a lively settlement that finally closed in 1996 with all the houses being dismantled and sold off. In 2006 the area was bought by an entrepreneur who subsequently replaced the houses with houses of the earlier eras and furnished them with items from the same period. ...