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Zhongjing Holiday Hotel
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LuShan Mountain
... than a few butt-clenching moments going up the mountain, as he overtook cars, buses, trucks and anything else on the road (which was like a snake, with that many blind corners it was ridiculous) and cut them off with a swift manoeuvre that would have impressed the guys from Top Gear no end. So we finally got to the top of the mountain, checked into our hotel (another one) and proceeded to go for a 'short walk' around the village and to the mountain. The village ...
It rained, I went home early
... and freezing. There was no way I was going to stay in Lushan for the night, feeling this grumpy. Attempting to get back home was better than not attempting to get back home at this point.
The driver drove the whole way down the mountain with the windows down. Fffffff..... Come on REALLY?! Then he dropped us off at least a 20 minute walk from the train station, which I did in some more pouring rain.
When i got there, The ticket woman yelled at me in ...
It is not a device for holding records
The hostel isn't very nice. The bathrooms, dorms, and beds leave a lot to be desired. And given that we are paying what we were paying two nights ago, it seems a bit ridiculous. But there really wasn't much choice. When we first arrived at our hotel two nights ago, we requested three nights. Only two, she said. Blast! So we went on a long, fruitless search for a hotel that would take us on the night of 1 October. ...
Life Fades Away
... to flee the mainland completely; the Communists may have found plenty of fault in the man, but he certainly knew how to party and they did, too, taking over Chiang's villa and holding a few big conferences here. Today, though you can check out some of these old conference spots, the real draw is what it was over 100 years ago: the scenery.
That's not to say you're in the middle of nowhere. This is China. There's a full-fledged town up ...
Week 2- Venturing to Deng Feng & Zhengzhou
... As i was sitting on the bus i was in absolute awe of this place. The amount of people there was amazing, all roughly the same height with black hair. Hundreds of people on motorised bikes with babies and children on the back, sometime up to three children squished on, holding on for dear life in such heavy traffic. They don't wear helmets over here either and just trying to cross the road is a matter of life and death. I'm still trying to get to grips with ...


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The Ferrari Tribute to the Targa Florio Has Begun
Over 50 of the Prancing Horse's vehicles are celebrating the 100th anniversary of the race The Ferrari Tribute to Targa Florio was officially opened with a starting ceremony in Piazza Giuseppe Verdi. Over 50 of Ferrari's vehicles will ride along the streets of Sicily ahead of the Targa Florio...Read More
Ferrari Tribute to Mille Miglia
The streets of Desenzano provided the venue for the start of both the Mille Miglia and the Ferrari Tribute to "the world's most beautiful road race" as Enzo Ferrari liked to dub it. A total of 20 historic Ferraris lined out for the former with 45 contemporary Ferraris fielded for the latter. Two...Read More

subset: commoncrawl


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Free personalized radio that
plays the music you love
Yasunao Tone & Hecker
Although he graduated with a degree in Japanese literature in 1957, three years later he encountered Group Ongaku, who improvised and put on "event music" and began participating himself in 1962, starting with a solo concert. He has also participated in the groups Hi-Red Center and Team Random, purportedly the first Japanese computer art group. In 1966, Team Random hosted a process music festival, which included Tone's "Theater Piece for Computer."
After relocating to the U.S. in the early '70s, Tone continued to perform (at New York's Roulette and the Kitchen, among others), participate in Fluxus concerts, and compose for the Merce Cunningham Dance Company. One of his works for a Cunningham dance, "Geography and Music," was also played at several new music festivals, including the John Cage Festival in San Juan. He has received numerous grants and commissions for his work. Some of his compositions have been recorded for independent labels such as Lovely Music and Tzadik. ~ Joslyn Layne
Selected Discography
Track List: Palimpsest
Similar Artists
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Top Definition
when hitting a chick from behind, about to pop, you slap her ass cheeks into your meat (masculine sex organ)creating a explosive sensation in the anus.
Yo last night i gave her a total meatsicle sandwich.
He gave me a meatsicle sandwich and the come came out my mouth.
He gave me a meatsicle sandwich and the come came out my mouth.
by skeetshooter44 April 30, 2008
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SA and Australia resume a long-standing rivalry when the two southern hemisphere giants clash in Sunday's RWC quarter-final.
And then there were eight! South Africa and Australia resume a long-standing rivalry when the two southern hemisphere heavyweights clash in Sunday's Rugby World Cup quarter-final at Wellington's Regional Stadium.
It's a mouth-watering encounter, but one that was never anticipated before the start of the tournament until Ireland split the road to the final into a southern and northern hemisphere highway after shocking the Wallabies (and perhaps everyone else for that matter) in their Pool C hit-out three weeks ago.
Australia's 15-6 defeat at the hands of the Irish effectively consigned them to a do-or-die battle against reigning champions South Africa, who finished the group stage of the competition unbeaten.
The South Africans booked their place in the play-offs after scraping through their opening game against Wales, but they looked far more impressive in the wins over Namibia, Fiji and Samoa.
Keeping things tight has not only given the Springboks those four wins out of four, but also the best defensive record from the pool phase – conceding just 24 points, ten fewer than the next stingiest defences of England, Ireland and Wales. Not conceding points is a good habit for teams to get into, as all previous World Cup winners have been among the top five defensive sides at the end of the group stages.
The Wallabies (48 points conceded) have had a more turbulent time in progressing to the knockouts – a win over Italy in the second half and a loss to Ireland, was followed by costly victories against the USA and Russia that saw two players sent home injured and several more in the casualty ward.
The Boks haven't had the pleasure of side-stepping injuries of their own and suffered a major blow to their campaign when losing in-form centre Frans Steyn and, most recently, veteran lock Bakkies Botha.
The injury-hit outfits must now pick themselves up for one of the most eagerly awaited World Cup spectacles of the weekend, which after 80 minutes will decide who stays on to fight another day and who goes home.
The Springboks have lost five of the last six Tests against their Tri-Nations counterparts, but as shown many times on the World Cup stage – previous results mean absolutely nothing when there's a semi-final at stake. And on neutral ground, this game is certainly anyone's for the taking.
Both teams have been crowned world champions twice, and are gunning for a record three championship titles.
The last time these sides met at a World Cup – in 1999 – South Africa were defending their crown only to be knocked out in the semi-finals by Australia (who went on to lift the Webb Ellis Cup for a second time – a record back then).
Fast forward 12 years later, and again we find ourselves in a similar scenario: defending champs South Africa versus the Wallabies in a knockout match, however this time a place in the last four will be up for grabs.
History sure has a funny way of repeating itself, but not unless the wise old owls of the Springbok team have anything to say about it when they tackle the baby-faced Wallabies who, with the youngest squad in the tournament, will take on the most experienced Test team of all-time.
Only six members of Australia's matchday 22 played in the 2007 quarter-final exit to England in Marseille, whilst the rest of current squad played no part in the tournament four years ago.
South Africa's selection includes eight players who started in the 2007 Rugby World Cup Final as well as three players who were part of the squad. Only Pat Lambie, Morne Steyn and Heinrich Brüssow of the starting team were not involved in 2007, while Pierre Spies was originally selected but had to withdraw due to illness.
But whilst South Africa can boast more World Cup caps on their CV, a full-strength Wallabies outfit is always a dangerous one and the return of David Pocock, Stephen Moore and Digby Ioane – three influential players that missed the Ireland game – will be a welcome boost for Australia.
The Wallabies know their shock loss to Ireland has left them walking the finals tightrope in the horror southern hemisphere side of the draw, but the Tri-Nations champions will draw much confidence from the return of the above mentioned trio.
It also means that with a full-strength line-up back on deck for the Australians, coach Robbie Deans must accept there can be no excuses for a disastrous World Cup quarter-final exit.
Speaking of coaches, Peter de Villiers faces the biggest challenge of his four-year career as boss of the Springboks. The thin line between success and failure has never been as apparent as now.
"Australia won't be easy, but they will be easier," said De Villiers.
"There are two reasons for this. Firstly they won't be as physical as Samoa, but they will try to be. It is just Samoa who can be as physical as that, because it's part of its nature.
"Secondly, we understand Australia and we know what they do. We've been playing them for years, and they're not likely to change anything this coming week. For them it will be easier and for us it will be easier."
The Boks have made no secret that their setpieces will be the basis for their attack, and armed with a stingy scramble defence, they will feel assured that they have the firepower to beat the Wallabies.
However, whilst the men in green and gold pack a punch up front, the Wallabies can deal a knock-out blow with their ruthless backs who ran South Africa ragged in Sydney two months ago – albeit against a second-string Bok team.
But whether it's up front or in the backs, one thing's for sure is that there will only be few opportunities to win this match come kick-off on Sunday and the team that is up for it and ready to take those chances will be the one that will be staying.
Ones to watch:
For South Africa: The big talking point leading into this quarter-final was the loss of Frans Steyn and how South Africa's back-line will cope without his physical presence on attack and defence. Jean de Villiers was the first-choice centre before his injury against Wales, and now returns to the Bok midfield with some mighty big boots to fill. In the forwards, the injury to Bakkies Botha could be a blessing in disguise for SA as it keeps big Danie Rossouw – arguably the Springboks' player of the tournament – in the starting line-up.
For Australia: In the backs, keep an eye out for tackle-busting wing Digby Ioane who returns to the fray after recovering from a broken thumb. With the wet weather that has been hanging over Wellington this week set to clear by Sunday, the Wallabies will be intent of getting the ball wide to their strike runner more often than not. Up front, Stephen Moore will make sure the Aussie scrum (that took a battering against the Irish), only goes one way when the two front rows lock horns in the New Zealand capital.
Head to head: Heinrich Brüssow v


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Keep it clean
If you believe that a wish duplicates another one or is not meant for the category, use Options button above to report a duplicate or spam.
Add your wish
If there is an item you wish to have on GOG.com and it's not yet on the wishlist, please add your wish
Theme Park Inc
5 comments about this wish
If we can have Theme Park i think we can get this
I would absolutely love you to the moon and back if you could add this game. I have insane cravings to play this a couple of times every other year.
I think this game supposed to be included for GOG as it is Bullfrog's final Theme Park game until EA had the nerve to completely take over Bullfrog and have control for themselves.
This was the best of the theme park games!!! its still in shops!
 However theres a huge bug that m,ake the savefiles corrupt on every system ive used in my life and several copies, please fix it and let me buy it!
Also known as SimCoaster in US. And yeahm, I'd love to have it too: http://www.mobygames.com/game/windows/simcoaster


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1851 – St. Patrick's Seminary, Maynooth, Co. Kildare
To appease the Irish bishops after the foundation of the three "godless" colleges in Dublin,
To appease the Irish bishops after the foundation of the three "godless" colleges in Dublin,
Designed in early Norman Gothic style, architect unknown for Rev. Timothy Kavanagh, PP. The foundation stone was laid 1847 by Rt.
These drawings are from the Narrative of Operations in the Arrangement and Formation of A Camp for 10,000 Infantry on the Curragh of Kildare.
Fine brick water-house with cast-iron tank above for the provision of water to railway engines in the steam era.
Geraldine Hall, designed by E. McAlister and originally built as a Church of Ireland School. It retains many important early or original features and materials,
Colclough was a civil engineer and architect, of Dublin, who died at the age of thirty-three on 21 June 1880 and was buried in Mount Jerome cemetery.
In 1857 John La Touche first heard the preaching of Dr. Spurgeon in London and was gradually won over by his brand of Baptist evangelism.
Constructed along with a small Baptist Church by John La Touch. "The glebe-house is built of grey stock brock with Corsehill stone dressings."
Design by William Isaac Chambers selected in limited competition. Published in The Irish Builder, March 1 1882.
"Our illustration in this number represents a new cottage in course of erection at Monasterevan, County Kildare,


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Home > Merchandise > Plushies > Ghost in the Shell SAC: Tachikoma Plush (Yellow)
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FREE SHIPPING over $60
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Category Archives: High School
2016-2017 Honor Roll for the 1st Quarter
RTI at BHS!
RTI is the abbreviation for Response to Intervention. Response to Intervention simply refers to a system where supports are in place to assist students, ensure academic success, and prevent academic failure. An RTI period of 38 minutes will be incorporated into the daily schedule at BHS this year to provide students time to receive additional help, complete missed assignments, and even explore additional learning opportunities. Each student will be assigned a mentor with whom they will meet each Monday during the RTI block. The mentor will assist students in determining which teachers to see Tuesday through Friday and with scheduling meetings with guidance counselors, administrators, and other school personnel. We believe this will impact Brewer High School tremendously this year and look forward to providing students with additional support!


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Informatica Lead Developer with DB2 ,Complex mappings
- Los Angeles, CA
- Contract
Company Description
Please share resumes at firstname.lastname@example.com,email@example.com reach me at 425-533-9681 Ext:104
Sr Informatica Lead Developer- Iowa - 6 Months
Job Description
Sr Informatica Lead Developer- Iowa - 6 Months
Skills Informatica Development n Design , Oracle, SQL Server, DB2, develop complex mappings, UNIX
We are looking For ETL Lead at least for 3 years with some exp in on-shore off shore development.
Informatica Lead developer with data migration project (not developer) it needs to be Lead developer where he has managed 4 ETL resources in a team.
If they have Infa Certification that is good as well.
Data migration experience -needs have experience with customer data merge or disparate data sources
Also dealt with On-shore -off shore development models.
I have enclosed a profile as Senior Developer with Certifications but they are still looking for Lead Resource
Required Skills: Provide ongoing support and enhancements to ETL development and associated Data Warehouse processes. Support may include ETL development, unit testing, fixes, system testing, maintenance, querying data to verify results, and documentation Analyze routine business problems and requirements to provide technical support (coding, testing, implementation, and maintenance) for the ETL (Extract Transform and Load) and associated Data Warehouse processes required to run Data Warehouse products.Document findings and communicate findings through written documents and models to stakeholders. Experience performing Business Intelligence (BI) or Data Warehouse Development (Analysis, Design, Programming, Testing) Preferred 10+ years of experience with ETL (Informatica) Development/ Onshore-offshore Experience with ETL Team Lead
Bachelors Degree Management Information Systems, Computer Science, Computer Engineering, Network Technology, Information Technology, Information Systems or related field Experience with Informatica Strong written & oral communication skills.


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Glow-In-The-Dark Shapes: Arrow
A good solution in case a power outage occurred or just in low-light places in general.
- Tape is 'energized' by any light source - the longer the exposure to light, the longer the glow time
- Shapes measure 2 inch or 3 inch respectively
Selected Material Details
Related Products
Glow-In-The-Dark Shapes
Glow-In-The-Dark Shapes: Dot
Glow-In-The-Dark Slip-Gard™ Floor Signs: Exit (Arrow)
Accessories: Sign-PAL Stand
Confined Space Permits: Sewer Entry Permit
Safety Sign
Detailed Description
Glow-In-The-Dark Arrows
Lead visible and safe pathways in low-light and emergency blackout situations.
Photo-luminescent marking arrows glow in the dark in a green-yellow color. Mark aisles, walls, escape routes, stairwells and exit areas for directional safety.
The marking tape is 'energized' by any light source - the longer exposure to light, the longer glow time. Good marking visibility in regular light as well, with a greenish-white appearance. Flexible 10-mil polyester with laminated gloss surface and permanent acrylic adhesive back with peel-off liner.


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Season 2 of the Overtime podcast is here! Listen to the first episode with Meg Robichaud now.
Hi, all how are you? I have been working on the interesting project last month and this is one of the solutions for the app menu It suppose to be clean and also I wanted to explore other solutions then side menus :) You will click on the "hamburger" icon you will get menu, and you can close it with to x or click on the blurry section.
Have a great day and let me know what do you think :)
Checkout more of my designs :)
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Show and tell for designers
What are you working on? Dribbble is a community of designers sharing screenshots of their work, process, and projects.
Copyright © 2009–2017 Dribbble LLC. All screenshots © their respective owners. Shipped from Salem, Mass. USA.


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Is this an outdoor attraction or activity?
Is this attraction a "must-see" location?
Would this be a good hot day activity?
Is this attraction popular with tourists?
Does this attraction provide visitors with a taste of the local culture?
Does this attraction require above average amounts of physical activity (long walks, climbs, stairs or hikes)?
Would this be a good cold day activity?
Is this attraction exciting, unusual, or risky to visit?
Does this attraction have good sunset views?
Would this be a good sunny day activity?
Thanks for helping!
it is amaizing view. if you enjoy nature and hike thats the one. you will be stuning with all this landscape!
3 Days in Rio de Janeiro
Guide to Rio de Janeiro Outdoors
Rio for Begginers
Hang gliding in Rio de Janeiro is one of the most popular wind sports practiced in Rio. You will have the unique sensation of flying like a bird, with a light breeze of tropical air in your face and surrounded by the beautiful mountains and beaches of Rio de Janeiro.
For the adventurous up for a tough hike this is a must while in Rio! Unfortunately we had bad luck with cloud cover so made it to the top without being able to see the view but the hike was still incredible and quite the adventure. Be sure to have more days to do this in case of bad weather...
This is my favourite scenic views of all my travels (28 countries) you must be fit and healthy as it is quite a exhausting climb approx 5-6 hours with stopping photos ect.. If you are not experienced in hiking you will definitely need a guide which I highly recommend and they take you to the best spots too which are...
Definitely give this a go if you visit Rio. It can be hard to some but really worth it. And you will be very proud of yourself.
If you like extreme sport, beautiful views, and amazing pictures this is the place to go. The hike and rock climbing is approximate 7 hours in total if you don't have much experience. If you're an experience climber, I'm sure it will be a lot less than that. I don't recommend this attraction for kids or the elderly. It's highly...
Since the Rio Olympics and the opening of the metro's new line 4 (yellow), one can now travel within walking distance of Pedra da Gavea for only 4.10 R$. Take the metro to the "Jardim Oceanico" station. From here, it's a 2km (10 minute) walk to the entrance of Tijuca national park and the trail that leads to Pedra da...
I took the trail they call it "P-4", which is sort of a shorter one, but a bit harder since there are some full vertical climbing spots we took without equipment. But it's ok.
That trail will take you to Garganta do Céu, a big plateau underneath a big cave on the side of Gavea Stone which will provide you...
This is a difficult hike and you should be in good shape to do it. There is a section of rock climbing so be sure to have equipment if you want to see the view. I didn't have ropes so I used a guide from Rio Natural and had a great time. Be sure to do this on a clear...
The last 30m need either a guide with a rope, or some strength and experience, the view is 100%. Go!
Also interested to hear if there are park closing times..?
Hola Equipo de Casa Helbing,
Por favor me pueden compartir un correo para escribirles, quisiera solicitar precios para 1 habitación para 2 personas desde el 02/01 hasta el 04/01. Estuve intentando, pero no obtuve precios.
Erick Ortega
See all questions


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Canucks sign GM Mike Gillis to extension; Is Alain Vigneault next?
|Gillis poses with his hardware from last season. (Getty Images)
In a move that isn't the biggest surprise you'll hear all day, the Vancouver Canucks have signed general manager Mike Gillis to an extension. The team announced the deal on Monday evening.
The Canucks were bounced unceremoniously early in the playoffs this year in just five games by the L.A. Kings. In a city like Vancouver, where the fans are so hungry for a Stanley Cup and the expectations were that they would finally get it this year, the reaction to the team losing that quickly wasn't positive, and it seemed as though many were advocating a new approach up and down the organization.
Obviously Gillis is coming back and, presumably, that speaks well for head coach Alain Vigneault to return next season as well. His situation has been much murkier than Gillis', but Gillis appears to be one of Vigneault's big backers in the organization, so if Gillis gets an extension it would seem to be a good sign for Vigneault too.
"I am very pleased to be signing this extension to remain the President & General Manager of the Vancouver Canucks," Gillis said. "I am proud of the work our entire organization has done these past four years and look forward to continuing to build on our success. We are driven every day to try and win the Stanley Cup and will continue to focus on being a strong organization both on and off of the ice."
I was starting to feel like Kevin Bacon in Animal House: "All is well!" It wasn't the end of the world that the Canucks got bounced that fast, nor did it mean it was time to blow things up.
When cooler heads prevailed, it was easy to see that Gillis still deserves his position. He was, after all, named the GM of the Year just last season. His team is still built well and was good enough to have the best record in the league this season. Gillis is responsible for building that roster.
"These past four years have been the most successful in Canucks history. Mike and his management team have done an excellent job to position us for long-term success on and off of the ice," Canucks Sports & Entertainment Chariman Francesco Aquilini said. "We believe Mike's presence in this organization is important to the long-term success of this hockey club, and we are confident that we will continue to compete at the highest level under his leadership."
For more hockey news, rumors and analysis, follow @EyeOnHockey and @BrianStubitsNHL on Twitter and like us on Facebook.


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What's the active ingredient in Spectracide bait? I haven't tried that. I
prefer IGRs since they're less of a hazard to other critters.
The main advantage that I can see to baits is that you don't normally see a
mound until it has upwards of 50,000 ants. That's a lot of the biting
beasts. If you bait, you can get the small colonies before you even know
they're there.
----- Original Message -----
From: "james singer" <firstname.lastname@example.com>
To: <firstname.lastname@example.com>
Sent: Thursday, August 04, 2005 2:50 PM
Subject: Re: [CHAT] RoundUp/Surflan
> Agreed. I'm using Spectracide bait this time around. Seems to keep them
> under control.
> n Aug 4, 2005, at 1:46 PM, Daryl wrote:
> > Glad it's working for you despite the color.
> >
> > Yup, the orange is ugly for a bit, but it sure helps to know where
> > you've
> > sprayed - both intentionally and unintentionally.
> >
> > I just use Amdro, Award or Logic bait for the fire ants, broadcasting
> > it in
> > spring and fall. We've only had two fire ant mounds this year, and
> > both were
> > outside the fence, where my neighbor thinks he's helping (not) by
> > sprinkling
> > the mounds with Orthene. The baits work so much better than mound
> > treatments
> > that I don't even use the mound treatments anymore.
> >
> > d
To sign-off this list, send email to email@example.com with the
message text UNSUBSCRIBE GARDENCHAT


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Ronnie Magri is a noted American drummer, DJ, music producer and SAG-AFTRA actor who can be heard and seen in many different musical circles. His style of drumming and work is varied and he has been associated with artists in the Rock, Jazz, Punk, Rhythm & Blues and Burlesque genres.
"Welcome to my website! As I continue to record and perform with a wide range of groups and projects, please check back for my latest going-ons. I hope you enjoy this site, find it useful and keep checking back for updates or to discuss future projects and collaborations".
Ronnie Magri
New York, NY
Want to hear Ronnie's latest music and going on's in the studio? Join him at SoundCloud and hear them first. From Lounge Grooves and Brazilian Beats to Cinematic Raunchiness & Funky Fusion... you're not going to want to miss a beat!
The Critically Acclaimed Solo Album from Ronnie Magri
"The music on the CD is uniformly excellent... Magri and associates swing and they have fun doing it. You may have heard 'Moonglow' or 'Stormy Weather' or 'Mood Indigo' before, but never with such a sweltering, scintillating sense of satiny sleaze. "Offbeat Magazine New Orleans"


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We also need a website...with an online
>Yearbook with the malcontents'
As I look at what has taken place here, during the last 12 months, I am
more and more "struck' by the seeming fact, that the two main Hosta robins,
have tended to divide along political lines! hosta Open seems to be a home
for "rugged individuals" whareas Phenix seems to attract a considerable
percentage of "liberals" There is a tendancy for those individuals
(liberals)to form "packs, under the leadership of one or two "lead wolves".
Personally, I am able to think for myself (thank you) and don't really
need a big "brother"(sister)telling me what to do!
To sign-off this list, send email to firstname.lastname@example.com with the
message text UNSUBSCRIBE HOSTA-OPEN


text:
Americans are getting even more hard-core when it comes to driving after drinking, even if they are guilty of it themselves, a new online Harris poll finds.
Sixty-eight percent of those polled say it is dangerous or very dangerous to drive after only one or two drinks, the poll of 2,234 adults finds. One or two drinks is generally below what it would take to cross the 0.8% blood alcohol threshold that can land drivers in the pokey for drunken driving.
Yet more than a third, 37%, of those who drink alcohol say they've driven at a time when they've had too much to drink.
They're not the only ones who admit to being present for dangerous behavior behind the wheel.
Sixty-two percent say they have been in cars while the driver was talking on a cellphone that they were holding, 45% say they've been with drivers who were reading or sending text messages. About a fourth admit to still often or sometimes text in the car themselves. Thirteen percent say they've watched a video on a video or smartphone while behind the wheel.
It's not just technology that's causing distracted driving. There were 27% who admitted to "grooming while driving."


text:
Game app for iOS
- Status Closed
- Budget $15 - $25 USD / hour
- Total Bids 36
Project Description
The app for iOS will be a game in which, through successive levels, player will have to ask some questions in a given time.
10 different levels: same structure of game, but less time in every level. Possibility to add the time not spent in previous level to successive level.
Users should have possibility to buy time (i.e. packages of seconds) to play the game: 20 seconds, 50 seconds, 100 seconds.
Final result should be shared on Facebook, Twitter, Game Center.
Please indicate portfolio with similar projects developed. Only previous experience in game apps development will be considered.Get free quotes for a project like this
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text:
Explaining Variations in Breast Cancer Screening Across European Countries
This paper explores variations in the uptake of breast cancer screening and associated factors influencing utilisation of mammography screening among women aged 50 to 69 years in 13 European countries. We focus on the relative importance of individual (e.g. age, education, etc.) and institutional (e.g. public screening program) factors in explaining cross-country variation in the utilisation of mammograms. We take advantage of (a) newly available individual level data from the SHARE as well as (b) regional and country level data on institutional factors. We find that observed individual factors like age, education, health status, etc. are associated with screening uptake within countries but cannot statistically explain cross-country differences. In contrast, observed institutional factors like the availability of an organized screening program can statistically explain about 40 per cent of the between country differences in screening rates.
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|Oct 2012
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References listed on IDEAS
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- Dardanoni, Valentino & Wagstaff, Adam, 1990.
"Uncertainty and the demand for medical care,"
Journal of Health Economics,
Elsevier, vol. 9(1), pages 23-38, June.
- Valentino Dardanoni & Alan Wagstaff, 1987. "Uncertainty and the demand for medical care," Working Papers 028chedp, Centre for Health Economics, University of York.
- Antonio Giuffrida & Hugh Gravelle, 1998. "Paying patients to comply: an economic analysis," Health Economics, John Wiley & Sons, Ltd., vol. 7(7), pages 569-579.
- McGuire, Thomas G., 2000. "Physician agency," Handbook of Health Economics, in: A. J. Culyer & J. P. Newhouse (ed.), Handbook of Health Economics, edition 1, volume 1, chapter 9, pages 461-536 Elsevier.
- Vincenzo Carrieri & Marcel Bilger, 2013. "Preventive care: underused even when free. Is there something else at work?," Applied Economics, Taylor & Francis Journals, vol. 45(2), pages 239-253, January.
- Jinhu Li & Jeremiah Hurley & Philip DeCicca & Gioia Buckley, 2014.
"Physician Response To Pay‐For‐Performance: Evidence From A Natural Experiment,"
Health Economics,
John Wiley & Sons, Ltd., vol. 23(8), pages 962-978, 08.
- Jinhu Li & Jeremiah Hurley & Philip DeCicca & Gioia Buckley, 2011. "Physician Response to Pay-for-Performance: Evidence from a Natural Experiment," NBER Working Papers 16909, National Bureau of Economic Research, Inc.
- Grossman, Michael, 1972. "On the Concept of Health Capital and the Demand for Health," Journal of Political Economy, University of Chicago Press, vol. 80(2), pages 223-255, March-Apr.
- Chang, Fwu-Ranq, 1996. "Uncertainty and investment in health," Journal of Health Economics, Elsevier, vol. 15(3), pages 369-376, June.
- Byrne, Margaret M. & Thompson, Peter, 2001. "Screening and preventable illness," Journal of Health Economics, Elsevier, vol. 20(6), pages 1077-1088, November.
- Hendrik Schmitz & Ansgar Wübker, 2011. "What determines influenza vaccination take‐up of elderly Europeans?," Health Economics, John Wiley & Sons, Ltd., vol. 20(11), pages 1281-1297, November.
- Lairson, David R. & Chan, Wenyaw & Newmark, Georgina R., 2005. "Determinants of the demand for breast cancer screening among women veterans in the United States," Social Science & Medicine, Elsevier, vol. 61(7), pages 1608-1617, October.
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text:
I'm interested . However , I'm only willing to trade your Vickie Cr with my tanaerve cr (500k), Jackie Cr (220k), kalindar (75k)and Wee Lee (0exp) (20k+) . If you're fine with it , do hit me a secure trade and a message as well . Thanks in advance .
* Cards mentioned above are at full exp unless stated.


text:
I was so excited to get the LockPick CHR550 for so many reasons but it's been nothing but a disappointment. First let's just say I would have sent it back a long time ago had it not been for the fact that my Dodge center console and dash are such a pain to get apart I don't have time to do that lengthy project. I took it apart 3 times just to disconnect it to reset it. That works for about a month. The audio quality through the I-pod cable is awful. My back up camera stopped working completely. The navigation stopped working entirely. The CHR550 doesn't even show up half of the time on my system. I am and will be disappointed until I remove it completely. I just wish I could still get a refund.
Expert Service 1-661-257-9993 [Hours]


text:
For Sale:
Lawton Audio LA7000 with Paduak Cups - Full Mod - MINT in CANADA
Will Ship To: North America
Up for Sale are my Lawton Audio LA7000 (Full markl Mod -10ft. Copper Cable with 1/4" Neutrik - Glossy Paduak cups). These are a beautiful set of headphones.
These are the headphones I have enjoyed most and I have owned many (HD800, LCD-2 rev 1&2, HF-2)
They have amazing tight impactful bass, Smooth mids and vocals, and sparkling highs with little sibilance. Soundstage is the best I have heard in a closed headphone.
They have 100 hours on a recommended burn in of 200 hours, and are in MINT condition.
Also for sale is my Burson HA160DS in a separate sale for $800, which is a fantastic pairing.
All packaging, and stock cups included. Price Includes Shipping (Paypal Fees Extra)
Edited by floydfan33 - 3/30/12 at 3:11pm


text:
Leak suggests Samsung is going after the Surface Pro with an impressive tablet PC
And it might be coming very soon.
And it might be coming very soon.
It's like the Yoga Book, but with a bigger screen and more affordable.
And you might be able to buy one later this year.
The company says it's the smallest 2-in-1 ever.
The scheme nearly shut down emergency lines in three different states.
The Note7's dead features live on.
Bill Belichick has had a lot of obstacles to overcome in his day, but he's raised the white flag of surrender on this.
This $89.99 tablet is basically a solid, if unremarkable, portal to everything Amazon.
Google's fall 2016 hardware event could be the most important moment in the company's 18-year history.
And maybe replace the 9.7-inch iPad Pro with a slightly larger screen.
New Pixel phones, Google Home, Chromecast, and more!
Is it time for another 7-inch Nexus?
Cool doesn't even begin to describe this combination tablet, mini laptop and digital notebook.
A new study indicates that bringing an iPad to the hospital before a child's surgery may be a great idea.
Your work bag just got a lot lighter.
Can Chinese tech titan Huawei give Microsoft and Apple a run for their money?
Flexible tech is coming, folks.
If you want to see your children this summer, help the put down technology
The shop is perfect for being discreet, or if you just don't feel like talking.
Everyone's making a Surface Pro-like 2-in-1 hybrid now.
Feed your addiction to technology alongside that of fast food.
Apple iPad Pro attempts to hit home run with Major League Baseball.
Get an up-close look at the tablet's advanced display, excellent camera and improved speakers.
If you've been waiting to upgrade, Apple's best new iPad is finally here.
Apple's iPad Pro line just got another, more portable option.
Apple is expected to debut the 4-inch iPhone SE, a new iPad, Apple Watch bands ... and maybe something more. Mashable's live blog will cover it all for you.
Samsung's iPad Pro competitor still has more in common with Microsoft's Surface Pro.
If you don't expect a new 4-inch iPhone, then you haven't been paying attention.


text: Posted May 13, 2013 Laura Bleiberg reviews Los Angeles Ballet in a Balanchine triple bill: http://www.latimes.com/entertainment/arts/culture/la-et-cm-los-angeles-ballet-balanchine-20130512,0,1004477.story Los Angeles Ballet's Balanchine Festival continues with three stirring modern masterworks, two of which highlight the brilliant outputs of double giants of the last century.That duo would be George Balanchine, co-founder of New York City Ballet, and composer Igor Stravinsky, who forged a friendship and working relationship based on mutual admiration. Their rare collaborations of new dance to new score netted breakthrough ballets, an organic meeting of genius. Share this post Link to post


text:
83rd Texas Legislature, Regular Session, Senate Bill 690, Chapter 1331
Description: Bill introduced by the Texas Senate relating to the creation of Harris County Improvement District No. 23; providing authority to issue bonds; providing authority to impose assessments, fees, or taxes.
Date: June 14, 2013
Creator: Texas. Legislature. Senate.
Item Type: Legislative Document
Partner: UNT Libraries Government Documents Department


text:
Structural investigations of the ferredoxin and terminal oxygenase components of the biphenyl 2,3-dioxygenase from Sphingobium yanoikuyae B1
- Daniel J Ferraro†1,
- Eric N Brown†1,
- Chi-Li Yu2,
- Rebecca E Parales3,
- David T Gibson2 and
- S Ramaswamy1Email author
© Ferraro et al; licensee BioMed Central Ltd. 2007
Received: 11 October 2006
Accepted: 09 March 2007
Published: 09 March 2007
The initial step involved in oxidative hydroxylation of monoaromatic and polyaromatic compounds by the microorganism Sphingobium yanoikuyae strain B1 (B1), previously known as Sphingomonas yanoikuyae strain B1 and Beijerinckia sp. strain B1, is performed by a set of multiple terminal Rieske non-heme iron oxygenases. These enzymes share a single electron donor system consisting of a reductase and a ferredoxin (BPDO-FB1). One of the terminal Rieske oxygenases, biphenyl 2,3-dioxygenase (BPDO-OB1), is responsible for B1's ability to dihydroxylate large aromatic compounds, such as chrysene and benzo[a]pyrene.
In this study, crystal structures of BPDO-OB1 in both native and biphenyl bound forms are described. Sequence and structural comparisons to other Rieske oxygenases show this enzyme to be most similar, with 43.5 % sequence identity, to naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4. While structurally similar to naphthalene 1,2-dioxygenase, the active site entrance is significantly larger than the entrance for naphthalene 1,2-dioxygenase. Differences in active site residues also allow the binding of large aromatic substrates. There are no major structural changes observed upon binding of the substrate. BPDO-FB1 has large sequence identity to other bacterial Rieske ferredoxins whose structures are known and demonstrates a high structural homology; however, differences in side chain composition and conformation around the Rieske cluster binding site are noted.
This is the first structure of a Rieske oxygenase that oxidizes substrates with five aromatic rings to be reported. This ability to catalyze the oxidation of larger substrates is a result of both a larger entrance to the active site as well as the ability of the active site to accommodate larger substrates. While the biphenyl ferredoxin is structurally similar to other Rieske ferredoxins, there are distinct changes in the amino acids near the iron-sulfur cluster. Because this ferredoxin is used by multiple oxygenases present in the B1 organism, this ferredoxin-oxygenase system provides the structural platform to dissect the balance between promiscuity and selectivity in protein-protein electron transport systems.
Sphingobium yanoikuyae B1 (B1), previously known as Sphingomonas yanoikuyae B1 and Beijerinckia sp. strain B1 [1], was isolated by virtue of its ability to use biphenyl as its sole source of carbon and energy for growth [2]. B1 is capable of using naphthalene, phenanthrene, anthracene, toluene, m- and p-xylene as sole sources of carbon and energy [3]. Other compounds are also oxidized by this microorganism and many of these are converted to cis-dihydrodiols. B1 remains one of the only known microbes, along with Mycobacterium vanbaalenii PYR1 [4] and Sphingomonas sp. strain CHY-1 [5–7], able to oxidize large aromatic hydrocarbons such as benzo[a]pyrene, benzo[a]anthracene and chrysene [8] to cis-dihydrodiols.
In the B1 genome, at least six sets of putative oxygenase genes are present [9] and are all believed to share a common electron donor system [10]. The genes bphA1A2f, which encode BPDO-OB1, have been sequenced and found to encode a Rieske oxygenase (RO) [11–13] related to naphthalene and biphenyl dioxygenases [14]. BPDO-OB1 is responsible for the oxidation of large aromatic compounds, such as benzo[a]pyrene, by B1. Structural information [15–17] and molecular modeling [18] have been used to determine features important for substrate specificity in other biphenyl oxidizing oxygenases; however, most of the effort has been targeted at understanding how biphenyl dioxygenases catalyze the degradation of polychlorinated biphenyls (reviewed in [19–22]). Previous structural studies of several ROs provide insight into common features of how the terminal component of the RO systems are organized and how substrate interacts with the enzyme to form the hydroxylated product [23]. To date, structures of enzymes that catalyze cis-dihydroxylation of aromatic substrates with more than three rings have not been reported.
Rieske oxygenase systems have multiple components whose function is to transfer electrons from NAD(P)H to active molecular oxygen and ultimately oxidize aromatic hydrocarbon substrates [23]. A single reductase for the multiple Rieske oxygenases is present in the B1 genome [10]. This reductase passes one electron at a time from NAD(P)H to the Rieske ferredoxin, BPDO-FB1, which in turn passes the electron on to the dioxygenase enzyme [24]. The terminal oxygenase component is responsible for catalyzing the addition of molecular oxygen to the aromatic substrate. This occurs at the mononuclear iron, contained in a large, primarily hydrophobic active site. The residues that form the active site have been shown to control substrate specificity.
Here we report the structures of BPDO-FB1 and BPDO-OB1. The structure of BPDO-FB1 shows similarities and important differences compared to other known Rieske dioxygenase ferredoxins. Structures of BPDO-OB1, in both the native form and bound to biphenyl, are presented. These structures demonstrate how BPDO-OB1 binds substrate in the active site. We also discuss the similarities and differences of this terminal oxygenase to other RO terminal oxygenase structures that have been previously determined and how these differences play a role in substrate specificity and regio- and stereoselectivity of product formation.
Results & discussion
Ferredoxin structure determination
Summary of crystallographic data and refinement statistics
Ferredoxin (2I7F)
Oxygenase (native) (2GBW)
Oxygenase (biphenyl bound) (2GBX)
Space group
Cell parameters
a = b (Å)
c (Å)
Resolution (Å)1
43.15 – 2.75 (2.85 – 2.75)
Data collection
X-ray source
Wavelength (Å)
Total observations
Unique observations
Completeness (%)1
93.7 % (65.2 %)
95.3 % (98.1 %)
92.6 % (99.7 %)
8.2 (2.9)
8.2 (2.9)
9.6 (3.7)
5.21 (3.46)
4.48 (4.10)
6.12 (7.14)
0.121 (0.417)
0.083 (0.394)
0.104 (0.409)
Crystallographic refinement
Rfactor/Rfree (%)3
RMSD from ideality
Bond lengths (Å)
Bond angles (deg)
Oxygenase structure determination
The asymmetric unit contains the entire BPDO-OB1α3β3 hexamer and residues 6 – 454 of the α subunits and residues 5 – 174 of the β subunits were modeled into the electron density. The structure has been refined to a resolution of 1.7 Å with a final Rfactor of 18.8 % and an Rfree of 22.9 %. The loop region located at the entrance of the α subunit active site is disordered, with higher than average B-factors and low side-chain electron density. This region spanned from residues 220 – 240 with residues 235 – 239 being the most disordered. Corresponding regions in other ROs are also disordered [15, 26–30]. Lsqkab [25] was used to determine the superposition RMSDs of the three α and β subunits in the asymmetric unit. A few regions in the α subunit, residues 108 – 123 and 411 – 434, and in the β subunit, residues 77 – 85 and 139 – 148, had higher than average RMSDs when compared; however, electron density clearly defined the coordinates of these residues. Crystallographic statistics are reported in Table 1.
Structure of the substrate-free oxygenase enzyme
The α subunit consists of 454 residues, which form two domains. The N-terminal portion consists of a Rieske [2Fe-2S] iron-sulfur cluster domain, defined by residues 40 – 140. The Rieske domain consists of β strands and loops, forming an ISP domain fold [31]. Four residues, two histidines and two cysteins, in the α subunit coordinate the Rieske non-heme iron cluster [2Fe-2S]. His-82 and His-103 coordinate one iron, while Cys-80 and Cys-100 coordinate the other. The C-terminal domain is a mix of helices and strands forming a TBP-like or helix-grip fold and is a member of the Bet v1-like superfamily [31]. The structural conservation suggests that the intraprotein electron transport in BPDO-OB1 is similar to the system described for NDO-O9816-4 and benzoate dioxygenase [32–37].
The β subunit of BPDO-OB1 has a Cystatin-like protein fold and belongs to the protein superfamily of NTF2-like proteins [31]. The function of the β subunits of ROs is not well understood and reports vary as to whether or not mutations in the β subunit of ROs can influence regioselectivity of product formation (reviewed in [43]). In the case of BPDO-OB1, the β subunit is not directly involved in creating the topology of the active site; however, the mononuclear iron is within 11 Å of the α-β subunit interface. This distance may allow select β-subunit side chains to indirectly affect the topology of the active site by interacting with residues that directly form the active site.
Structure of the biphenyl bound oxygenase enzyme
Comparison of BPDO-FB1 to other Rieske oxygenase ferredoxins
Sequence and structural statistics for various Rieske oxygenases compared to biphenyl 2,3-dioxygenase
Ferredoxin (PDB ID)
RMSD (Cα)
Sequence identity
Sequence similarity
1.017 (97)
36.6 %
56.4 %
1.697 (93)
24.1 %
52.7 %
1.312 (98)
36.3 %
60.8 %
Terminal oxygenase α Subunit (PDB ID)
RMSD (Cα)
Sequence identity
Sequence similarity
1.24 (420)
43.5 %
62.0 %
1.23 (413)
41.2 %
59.4 %
1.48 (388)
39.6 %
55.1 %
1.50 (377)
36.8 %
52.7 %
1.59 (358)
35.0 %
52.5 %
Terminal oxygenase β Subunit (PDB ID)
RMSD (Cα)
Sequence identity
Sequence similarity
1.19 (156)
28.1 %
46.0 %
1.19 (160)
29.5 %
47.0 %
1.15 (159)
31.6 %
53.2 %
1.17 (161)
31.8 %
48.6 %
1.20 (156)
35.3 %
49.3 %
Structurally analogous residues near the iron-sulfur cluster in various Rieske dioxygenase ferredoxins
Rieske cluster coordinating residues
Outer shell residues
Comparison of BPDO-OB1 to other Rieske oxygenases
The BPDO-ORHA1 (PDB entry 1ULI) active site has a significantly smaller volume, ~27 Å3, than BPDO-OB1, ~43 Å3. This is due to bulkier side-chains in BPDO-ORHA1 and variability in the loop positions at the active site entrance. Comparing the ligand bound structures, biphenyl carbons 2 and 3 are closer to the mononuclear iron in BPDO-ORHA1, 4.3 and 4.6 Å respectively, as compared to BPDO-OB1 which has an average distance of approximately 5.0 Å for both the 2 and 3 carbons in the three subunits. However, in both BPDO-ORHA1 and BPDO-OB1, the distances between the biphenyl and the iron-bound water are similar. The closer position in BPDO-ORHA1 is mediated by an interaction with Met-222, which is not present in BPDO-OB1. The glycine (Gly-205) present in the structurally analogous position leaves room for the ligand to move slightly further from the iron. The lack of large side-chain rearrangement upon substrate binding is similar to results seen in NDO-O9816-4 [27, 40] and BPDO-ORHA1 [16]. The loop covering the active site entrance of BPDO-OB1 shifts upon binding of biphenyl. While large changes in this loop are not seen in the structures of NDO-O9816-4, this loop is believed to be flexible in NDO-O9816-4[27, 40]. BPDO-ORHA1 does show significant movement of residues 271 – 276 shifting 1 – 2 Å toward the active site, with the side-chain Leu-274 forming van der Waals interactions with the ligand [16]. Loops covering the active site in the α3 Rieske monooxygenase 2-oxoquinoline 8-monooxygenase also demonstrate changes upon substrate binding [30]. This loop motion observed in the BPDO-OB1 and other RO structures allows the active site to "breathe" to accommodate ligands and may be one of the key features that allows this class of enzymes to perform catalysis on diverse sets of substrates with respect to overall size and shape.
Role of oxygenase active site entrance
Crystal structures of BPDO-FB1 and BPDO-OB1 from Sphingobium yanoikuyae strain B1 are presented and demonstrate strong structural conservation with other RO ferredoxin and oxygenase components. The structures reported here provide a rational basis for the ability of BPDO-OB1 to catalyze large aromatic substrates. It also provides a framework to interpret the product regioselectivity of BPDO-OB1 and the differences in product regioselectivity compared to other ROs. While the Rieske ferredoxin structure is very similar to other Rieske ferredoxins, the differences in amino acid composition near the cluster in BPDO-FB1 provide a unique opportunity among the Rieske oxygenase ferredoxins to examine the effect of cluster environment and hydrogen bonding on reduction potential.
Protein expression, purification and crystallization
BPDO-OB1, bphA1fA2f [11, 14] and BPDO-FB1, bphA4 [10], were each cloned from B1 into the protein expression vectors pET101D (Invitrogen, Carlsbad, CA) and pT7-7, respectively, and expressed as described in Yu et al. [45]. Crystallization of the ferredoxin protein was performed using 33 mg/mL of BPDO-FB1 protein in a 50 mM phosphate buffer, pH 6.8, with 0.1 M citric acid and 1.6 M ammonium sulfate as precipitants. Crystallization of the oxygenase protein was performed using purified BPDO-OB1 protein at 20 mg/ml in a 20 mM potassium phosphate buffer, pH 6.8 [45]. Mineral oil was used as a cryoprotectant for the crystals.
Oxygenase crystals were also used for soaking experiments in an attempt to generate the BPDO-OB1 protein-biphenyl complex. Soaking experiments were based on similar experiments previously done with NDO-O9816-4 to generate crystals of ligand-bound enzyme [27, 38, 40]. Crystals were moved to fresh drops containing reservoir buffer. Ethanol saturated with biphenyl was added to these drops. The crystals tolerated up to 5% ethanol in the drop. Crystals were allowed to soak for 3–5 days, then were removed from the drop and flash-cooled to 100 K. Mineral oil was used as a cryoprotectant.
Data collection, processing, structure solution and refinement
X-ray diffraction data for BPDO-FB1 were collected on beamline X6A at Brookhaven National Laboratory and data for BPDO-OB1 were collected on the IMCA-CAT beamline 17-ID at the Advanced Photon Source in Argonne National Laboratory. Crystallographic statistics are presented in Table 1. d*TREK [53] was used to process the data for BPDO-FB1 to a resolution of 1.60Å. Analyzing systematic absences, the space group was determined to be either P6122 or P6522. Molecular replacement using AMoRe [54] and a polyalanine model based on BPDO-FLB400 (PDB entry 1FQT) produced a solution in space group P6522. Model building using O [55] and Coot [56], density modification using DM, and refinement using Refmac5 [57] from the CCP4-4.0 program suite were assisted by non-crystallographic symmetry between the two monomers in the asymmetric unit. These NCS restraints were loosened as refinement progressed. Cycles of Refmac5 with ARP/wARP [58, 59] or the Coot find waters routines were used to identify solvent atoms. Asp-96 in chain B was modeled as having two side chain conformations. A single round of TLS optimization was used at the end of refinement with each protein monomer acting as a TLS group.
Data collected from native BPDO-OB1 crystals was processed using d*TREK [53] to a resolution of 1.70 Å. Analysis of systematic absences suggested that the space group was P3x21. Molecular replacement was performed using a polyalanine model based on NDO-O9816-4(PDB entry 1NDO) [28]. Molecular replacement using AMoRe [54] gave a clear solution in the space group P3121. Initial refinement of the polyalanine model with the program Refmac5 [57] of the CCP4-5.0.2 [60] suite of programs yielded good starting electron densities. The molecular visualization program O [55] was used for model building. After the bulk of the structure was modeled, refinement was continued with Refmac5 without NCS restraints. Solvent molecules were found using the program Arp/Warp [58, 59] and multiple side-chain conformations were modeled using the molecular visualization programs XtalView [61] and Coot [56]. The calculated solvent content was 50 % [62]. The asymmetric unit contains a complete α3β3 protein.
Data from biphenyl soaked oxygenase crystals were processed using d*TREK to a resolution of 2.8 Å. The native BPDO-OB1 structure, with solvent molecules removed, was used as a starting point for the refinement of the ligand bound structure. An energy-minimized structure of the small molecule biphenyl was constructed using the program SYBYL 7.1 [63]. This model was used to create a refinement dictionary for Refmac5 using the ligand sketcher program in CCP4-5.0.2. After initial refinement of the protein model, the biphenyl ligand was modeled into the active site of the enzyme where electron density maps showed un-modeled density in both 2Fo-Fc and Fo-Fc maps. The torsion angle between the two rings of biphenyl was allowed to rotate during refinement and solvent molecules were modeled as appropriate.
Sequence and structural alignments
TCoffee [64] was used to produce a structure-based sequence alignment of the four known RO ferredoxin structures and 19 other RO ferredoxin sequences. Structural alignment of native BPDO-OB1 with structures of other RO oxygenases was performed using the program Indonesia [65]. Structural alignments in Indonesia were done pairwise using the Levitt and Gerstein method with a cut-off value of 3.5 Å. Sequence alignment and comparison was done using full sequences for each of the proteins without structural information. Protein-protein sequence alignments were performed using blastp on the NCBI blast server [66]. All figures showing structures were created using PyMOL 0.98 [67]. Figure 6 was produced using a modified version of LigPlot [68].
Rieske Oxygenase
- BPDO-OB1 – Biphenyl 2:
3-dioxygenase from Sphingobium yanoikuyae strain B1
- BPDO-FB1 – Biphenyl 2:
3-dioxygenase ferredoxin from Sphingobium yanoikuyae strain B1
- BPDO-ORHA1 – Biphenyl 2:
3-dioxygenase from Rhodococcus sp. strain RHA1
- NDO-F9816-4 – Naphthalene 1:
2-dioxygenase ferredoxin from Pseudomonas sp. strain NCIB 9816-4
- NDO-O9816-4 – Naphthalene 1:
2-dioxygenase from Pseudomonas sp. strain NCIB 9816-4
Sphingobium yanoikuyae strain B1
We thank Jon Mowers, Adam Okerlund, Nathan Coussens and Heather Hanson for critical reading of this manuscript and assistance with various experiments performed in this manuscript. We also thank Gerben Zylstra for providing clones of the B1bphA1A2f genes. Use of the IMCA-CAT beamline 17-ID at the Advanced Photon Source was supported by the companies of the Industrial Macromolecular Crystallography Association through a contract with the Center for Advanced Radiation Sources at the University of Chicago. Use of the Advanced Photon Source was supported by the U. S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. W-31-109-Eng-38. We would like to thank Lisa Keefe, Kevin Battaile and Irina Koshelev for help with data collection at the IMCA-CAT facility. Research carried out at X6A beam line, National Synchrotron Light Source, Brookhaven National Laboratory, is supported by the U.S. Department of Energy under contract # DE-AC02-98CH10886. X6A is funded by NIH/NIGMS under agreement Y1 GM-0080-03. We thank Vivian Stojanoff for help with data collection at X6A. D.F. and E.B. are U. of I. MSTP trainees and would like to acknowledge financial support through a fellowship from the U. of I. Center for Biocatalysis and Bioprocessing. D.F. would like to acknowledge a thesis-parts award by the Department of Energy Division of Student Programs for work at the Advanced Photon Source in Argonne National Laboratory. C.-L.Y would like to acknowledge financial support by National Science Foundation Engineering Research Centers Program grant # EEC-0310689. S.R. would like to acknowledge financial support from USPHS Grant # GM62904.
Authors' Affiliations
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