text:
A recombinant (r-) Salmonella typhimurium aroA vaccine that secretes the naturally secreted protein of Mycobacterium bovis strain BCG, Ag85B, by means of the HlyB/HlyD/TolC export machinery (termed p30 in the following) was constructed. In contrast to r-S. typhimurium control, oral vaccination of mice with the r-S. typhimurium p30 construct induced partial protection against an intravenous challenge with the intracellular pathogen Mycobacterium tuberculosis, resulting in similar vaccine efficacy comparable to that of the systemically administered attenuated M. bovis BCG strain. The immune response induced by r-S. typhimurium p30 was accompanied by augmented interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) levels produced by restimulated splenocytes. These data suggest that the HlyB/HlyD/TolC-based antigen delivery system with attenuated r-S. typhimurium as carrier is capable of inducing an immune response against mycobacterial antigens.
Tuberculosis (TB) continues to be a major cause of morbidity and mortality throughout the world, resulting in 8 million new cases and 2 million deaths each year [1]. The only TB vaccine currently available is the attenuated Mycobacterium bovis strain BCG, which has variable protective efficacy ranging from 0 to 85% in different controlled studies [2,3]. Therefore, the development of more potent TB vaccines is urgently required [4]. The causative agent of TB, Mycobacterium tuberculosis, is a facultative intracellular microorganism that misuses macrophages as its preferred habitat. The altered micro-environment in M. tuberculosis-containing phagosomes and the defects in maturation processes along the endosomal—lysosomal continuum cause impaired processing of mycobacterial antigens and diminished cell surface expression of major histocompatibility complex (MHC) class II molecules, which in turn reduces antigen recognition by immune CD4 T cells [5–8]. Nevertheless, the important role of CD4 T cells in the control of TB is unquestionable. These protective CD4 T cells are of the Th1 type, i.e. they are potent producers of interferon-γ (IFN-γ) (which induces anti-mycobacterial activities in macrophages [9]).
Secreted proteins of M. tuberculosis are recognized early in the course of experimental TB infection of mice and by T cells of TB patients, and they are therefore considered preferential antigenic targets for vaccine development [4,10]. The antigen 85 complex is expressed by nearly all mycobacterial species analyzed so far [11]. More importantly, p32A (antigen 85A or 32-kDa antigen) and p30 (antigen 85B or 30-kDa antigen) stimulate B and T cell responses in TB patients [12–14], and immunization of guinea pigs with a p30 antigen preparation together with adjuvant induced protective immunity against TB [15,16]. By means of DNA vaccination, both antigens, p30 and p32A, induced protection against aerosol challenge with M. tuberculosis in mice and guinea pigs, respectively [17–19]. The proteins of the antigen 85 complex, including p30 and p32A proteins of M. tuberculosis belong to a family of gene products with fibronectin-binding capacity and mycolyltransferase activity, which is involved in the final stages of mycobacterial cell wall assembly [11,20]. Sequence comparison revealed that p30 and p32A of M. tuberculosis exhibit 72.8% amino acid identity and that the cross-species homology between p30 of BCG and of M. tuberculosis is 99% [21–24].
BCG, M. tuberculosis and Salmonella typhimurium microbes persist within the phagosomes of infected macrophages [25]. From an immunological point of view, the intracellular habitat of these microbes influences antigen processing mechanisms of MHC class I and II pathways, ultimately leading to CD8 and CD4 T cell responses, respectively [25]. Intraphagosomal localization preferentially stimulates MHC class II-restricted CD4 T cells whilst egression into the cytoplasm as achieved by Listeria monocytogenes primarily induces MHC class I-restricted CD8 T cells [25]. Yet, the recently described S. typhimurium aroA strains secreting heterologous antigens stimulate CD4 and CD8 T cell-mediated immunity [25–27]. Central to this mode of antigen delivery is the secretion of heterologous fusion proteins by means of the plasmid-encoded HlyB/HlyD/TolC translocator of hemolytic and uropathogenic Escherichia coli strains [25,28]. The latter directs protein secretion via recognition of the C-terminal hemolysin (HlyA) signal peptide across the cell wall of Gram-negative E. coli or Salmonella sp. microorganisms [28]. The recombinant (r-) Salmonella-specific antigen delivery system expressing defined antigens of L. monocytogenes induced both CD4 and CD8 T cells, which adoptively conferred protective immunity against listeriosis in mice [26]. This Salmonella-based antigen delivery device is gaining interest as a possible r-carrier against intracellular bacterial pathogens like M. tuberculosis[25,29].
A fusion protein was constructed encompassing p30 of M. bovis BCG and the C-terminal part of HlyA of E. coli, respectively. Here, we report on the in vivo immunogenicity of S. typhimurium p30 carrier strain and on partial protection against TB in C57BL/6 mice vaccinated orally with this r-construct secreting p30 of M. bovis BCG.
Materials and methods
Bacteria and plasmids
The S. typhimurium SL7207 strain (2337–65 (WRAY) hisG46ΔaroA407 (TcsaroA544::Tn10)) was kindly provided by Dr. B.A.D. Stocker (Department of Medical Microbiology, Stanford University, Palo Alto, CA, USA) [30]. The r-S. typhimurium strain pMOhly1 (termed control) has been described previously [26]. M. bovis BCG strain Chicago (ATCC 27289) and M. tuberculosis H37Rv (originally obtained from Dr. J.K. Seydel, Forschungsinstitut Borstel, Germany) were passaged in mice and then in Dubos broth base (Difco) supplemented with 10% Dubos medium albumin (Difco) and stored in aliquots at −70°C. The mycobacteria—E. coli shuttle vector pAB261 was kindly provided by MedImmune (Gaithersburg, MD, USA).
Construction of recombinant plasmid pAg85Bs
Plasmid pAg85Bs was constructed by the following cloning steps: BglII–HindIII DNA fragment of pANN202–312 (position 3811 and 5341 [31]) was inserted into pAB261 BamHI/HindIII ([21,32]; MedImmune, personal communication), the resulting plasmid was digested with NcoI and SwaI, and the obtained DNA fragment was cloned into pILH-1 vector cut by NcoI and SwaI restriction enzymes [26,33]. The final plasmid pAg85Bs was isolated from E. coli JM109, analyzed and electroporated in S. typhimurium LB5000, a restriction-negative and modification-proficient strain, by a standard protocol for E. coli (Bio-Rad, CA, USA). Subsequently, plasmids purified from ampicillin-resistant r-S. typhimurium LB5000 colonies were introduced into S. typhimurium SL7207 by electroporation. The resulting r-S. typhimurium p30 strain harboring plasmid pAg85Bs was grown in 2×yeast tryptone medium supplemented with 100 µg ml−1 of ampicillin (Sigma), 10 µg ml−1 each of 2,3-dihydroxybenzoic acid (Sigma, Deisenhofen, Germany) and p-aminobenzoic acid (Sigma, Germany) at 37°C. The r-S. typhimurium p30 or control were grown to an optical density (A600) of one. The bacteria were harvested and centrifuged at 3000×g. Each bacterial pellet was then resuspended in phosphate-buffered saline (PBS)/0.05% Tween 80. Acetone (−20°C) was added to the supernatant at a final concentration of 50% and subsequently stored at −20°C for 1 h. After this treatment, the solution was centrifuged for 30 min at 10 000×g and subsequently, the pellet was resuspended in water. The proteins were completely resuspended in water at the concentration of 8 mg ml−1. These antigen preparations of r-S. typhimurium p30 and control were adjusted to 2 µg protein per µl volume. Proteins prepared under reducing conditions of 7.5% sodium dodecyl sulfate (SDS) and separated by 10% SDS—polyacrylamide gel electrophoresis were electrophoretically transferred to a Hybond ECL nitrocellulose membrane, which was subsequently blocked overnight by incubation with PBS containing 1% bovine serum albumin. Immunostaining was performed with rabbit anti-p30 antibodies (1:5000) and anti-rabbit IgG antibodies conjugated with horseradish peroxidase (1:20 000). The Western blot was developed by means of an enhanced chemiluminescence detection kit according to the manufacturer's description (ECL Western blotting detection reagents, Amersham Pharmacia). For the generation of p30 antibodies, Specol adjuvant (Institute for Animal Science and Health, Lelystad, The Netherlands) together with the peptide H2N-GPSSDPAWERNDPT-CONH2 coupled at the N-terminus to keyhole limpet hemocyanin (Eurogentec, Seraing, Belgium) was used to immunize rabbits by a standard protocol (Eurogentec). The animals were immunized with 200 µg peptide per 1 ml adjuvant (10 injections with 100 µl each time point) by the intradermal route at days 0, 14, 28 and 56. Subsequently, the anti-p30 antibodies were enriched by binding to Protein A-Sepharose and then purified further by affinity chromatography on activated Sepharose (Amersham Pharmacia, Freiburg, Germany) conjugated with antigen 85 complex proteins p32A/p32B/p30 of M. tuberculosis H37Rv strain (generously obtained from Dr. J. Belisle, Fort Collins, USA).
Mice and infection studies
C57BL/6 mice were kept under specific pathogen-free conditions in isolators and fed autoclaved food and water ad libitum at the central animal facilities of the University of Ulm. In a given experiment, mice were age- and sex-matched. Previous studies on in vivo plasmid stability showed that all r-S. typhimurium SL7207 strains still harbored their pMOhly1-based plasmids by day 21 post infection (p.i.) [26]. C57BL/6 mice at 8–10 weeks of age were immunized per os (p.o.) three times with 5×109 bacteria per 200 ml PBS or intravenously (i.v.) with a single dose (5×105 bacteria) of r-S. typhimurium p30 or control strains at 5 day intervals. Ten weeks after the last injection, vaccinated mice were completely cured of r-S. typhimurium SL7207 infection, as verified by colony forming units (CFU) analysis on brilliant green Salmonella selective agar (Difco). At day 120 after the last vaccination, these mice were challenged i.v. with 106M. tuberculosis H37Rv bacteria. For control purposes, C57BL/6 mice were immunized i.v. with 106 BCG microorganisms for 120 days and challenged together with the group of animals which had received the r-Salmonella strains previously. At the indicated time points p.i., lung, liver and spleen of these mice were removed and homogenized with a lab blender (Seward Medical, London, UK). M. tuberculosis bacteria were enumerated by plating serial 10-fold dilutions in PBS on Middlebrook agar plates (Difco) supplemented with 10% OADC enrichment (Difco). After 3–4 weeks of incubation at 37°C, mycobacterial colonies were counted.
Stimulation in vitro and IFN-γ and tumor necrosis factor (TNF) production
At the indicated time points p.i., animals were killed and spleens removed. Single—cell suspensions were prepared in Iscove's modified Dulbecco's medium (IMDM) (Seromed, Berlin, Germany) supplemented with 10% FCS (Boehringer Mannheim, Mannheim, Germany), 2 mM glutamine, 100 U ml−1 penicillin/streptomycin (Gibco, Paisley, UK) and 1 µg ml−1 indomethacin (Sigma, St. Louis, MO, USA) (complete IMDM). Cells were seeded into 96-well plates (Nunc, Roskilde, Denmark) at the final concentration of 2×105 per well and cultured at 37°C in 7% CO2 in air. Cells were stimulated with the following mycobacterial antigen preparations (1 µg ml−1): (i) M. tuberculosis PPD (obtained from the Statens Serum Institute, Copenhagen, Denmark) diluted in PBS and (ii) freeze-dried M. tuberculosis H37Ra (obtained from Difco) suspended in IMDM. After culture for 3 days, cell supernatants were removed, sterile-filtered and frozen at −20°C until IFN-γ detection by enzyme-linked immunosorbent assay (ELISA). All experimental groups were analyzed in triplicate. IFN-γ was determined by double-sandwich ELISA using the monoclonal antibodies R4-6A2 and AN18-17.24, which recognize different epitopes of the cytokine as described [34]. Murine r-IFN-γ (generous gift of Dr. G. Adolf, Ernst Boehringer-Institut für Arzeneimittelforschung, Vienna, Austria), with a specificity of 107 U mg−1, was diluted in complete IMDM to obtain a standard curve. The detection limit of the ELISA was 0.05 U ml−1 IFN-γ. TNF was measured in a cytotoxicity assay using a L929-TNF-sensitive cell line [35]. Due to the use of the same TNF receptors (TNFR-I and II) for binding of TNF-α as well as TNF-β on the cell surface of L929 cells, this assay does not discriminate between both types of TNF. Nevertheless, murine r-TNF-α, specificity 8.3×107 U mg−1 (Genzyme), was used to obtain standard curves. Absorbance obtained by ELISA or bioassay was analyzed using the SpectraMax equipment (molecular Devices, Sunnyvale, CA, USA). Quantification of IFN-γ and TNF concentrations was done with SoftmaxPro software (Molecular Devices).
The attenuated S. typhimurium aroA SL7207 strain combined with the HlyB/HlyD/TolC export machinery of hemolytic E. coli isolates was used for delivering p30 of BCG as a secreted 63–kDa fusion protein. S. typhimurium p30 microorganisms were constructed, which harbored the pAg85Bs secretion vector encoding the p30 fusion protein (Fig. 1A). This p30 polypeptide consists of the N-terminal 44 residues of listeriolysin-HlyA encoded by pILH-1[33], followed by 14 random amino acids and the complete p30 sequence with 322 residues of pAB261 (MedImmune, personal communication; [21]) and ending with three linker residues and 190 amino acids of HlyA encoded by plasmid pANN202-312 downstream of the BglII site at position 3811 [31] (Fig. 1B). The 63-kDa p30 fusion protein is detectable in the supernatant of r-S. typhimurium p30 by immunostaining with affinity-purified rabbit anti-peptide p30 antibodies (Fig. 1C). This formally proves export of p30 by the r-S. typhimurium p30 antigen delivery system and suggests that the antigen is readily available for immune recognition.
In general, IFN-γ and TNF-α represent two prominent cytokines which contribute to anti-mycobacterial defence [36,37]. C57BL/6 mice were immunized with 5×105S. typhimurium p30 or control i.v., and at indicated time points, mice were killed and splenocytes were subsequently monitored for IFN-γ and TNF production. Splenocytes were restimulated with 1 µg ml−1M. tuberculosis H37Ra antigen preparation for 3 days in vitro. Splenocytes of r-S. typhimurium p30-vaccinated mice produced more than 2-fold higher concentrations of IFN-γ in comparison with spleen cells of mice that had received the r-Salmonella control strain (Fig. 2). Similarly, TNF release by splenocytes from mice immunized with r-S. typhimurium p30 was augmented. Nevertheless, after restimulation with M. tuberculosis H37Ra lysate, a strong cross-reactive immune response against the r-Salmonella control strain was observed (Fig. 2). We conclude that the r-S. typhimurium p30 construct enhanced the immune reactivity against p30 antigen compared to the r-Salmonella control strain.
For analyzing the protective efficacy of r-S. typhimurium p30, C57BL/6 mice were vaccinated p.o. and then challenged i.v. with M. tuberculosis H37Rv 120 days later. Sixty days post challenge, the bacterial burden was determined in different organs. For control purposes, groups of mice were immunized with the r-Salmonella control or M. bovis BCG and challenged with M. tuberculosis H37Rv. Animals were killed at day 60 post challenge and CFU were determined by plating serial dilutions of organ homogenates. As shown in Fig. 3, protection was induced by systemic BCG or mucosal S. typhimurium p30 vaccination, and was comparable in spleens and lungs of mice challenged with M. tuberculosis. At this time point, splenocytes of r-S. typhimurium p30-immunized mice showed significantly increased production of IFN-γ and TNF after in vitro restimulation with M. tuberculosis H37a lysate or PPD underlining, the enhanced anti-TB immune response compared with that in BCG or r-Salmonella control immunized mice (Fig. 4). We conclude from these results that immunization with r-S. typhimurium p30 primed a Th1-biased cytokine profile.
Our report describes protection against M. tuberculosis by oral immunization of mice with a r-S. typhimurium p30 carrier strain secreting the naturally secreted antigen p30 of M. bovis BCG. Furthermore, our data reveal that display of secreted antigens by the r-S. typhimurium carrier enhanced immune responses against M. tuberculosis as indicated by augmented IFN-γ and TNF production prior to and after challenge infection. This finding is consistent with the assumption that secreted antigens are central to the induction of protective immunity against intracellular pathogens [38–40].
From an immunological point of view, the secretion of p30 by the anti-TB vaccine M. bovis BCG as well as by r-S. typhimurium p30 represents a similar mode of antigen delivery. Both strains introduce antigen p30 from a vacuolar compartment within antigen presenting cells into antigen processing pathways. Therefore, it possibly should be noted that a single immunodominant antigen expressed by r-S. typhimurium possesses a similar protective efficacy against M. tuberculosis as does M. bovis BCG with its multitude of antigens recognized by M. tuberculosis-specific lymphocytes.
So far, only vaccinia virus has been used as a heterologous carrier for M. tuberculosis antigens such as the lipo-glycoproteins of molecular mass 38 or 19 kDa [41]. Taken together, our data show the feasibility of r-S. typhimurium carrier strains for inducing immune responses against TB, at least when an immunodominant naturally secreted antigen of M. bovis BCG with high homology to that of M. tuberculosis is delivered as secreted fusion protein.
This work was supported by the German Science Foundation, project Ka 573/3–1/2 to J.H. and S.H.E.K., and the BMBF project 'Mycobacterial Infections' to S.H.E.K., I.G. and W.G. received support from Grant Go168/16-3 of the German Science Foundation and FORGEN L1. We thank D. Miko and C. McCoull for expert help and we are grateful to MedImmune and Dr. B.A.D. Stocker for donating plasmids and S. typhimurium SL7207, respectively, to Drs. J. Belisle and E. Adolf for additional helpful reagents.


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It was always going to come to this. The Monetary Policy Committee threw up its hands last Wednesday and gave up the charade that all was going to be well by 2015. It finally had to admit its growth forecasts have been overly optimistic for years and is forecasting the UK economy is heading back into triple-dip recession. To quote from the Inflation Report: "In Q3, output increased by 1 per cent. In Q4, that growth rate seems set to fall sharply as the boost from the Olympics is reversed; indeed, output may post a small decline".
The growth forecast was considerably less optimistic than the one produced in August, which I criticised at the time for being from Cloud Cuckoo Land. And so it has turned out. The chart on the right above shows their latest forecast for growth in the form of their now infamous GDP growth fan chart, which is conditioned on Bank Rate remaining at 0.5 per cent and with £375bn of asset purchases. This shows that the MPC expects growth, shown by the dark swathe in the centre of the fan on the right, of only 2 per cent in 2015 compared with their earlier forecasts of 3 per cent and more. The MPC is forecasting that the drop in output of 6.3 per cent that occurred from 2008Q2 through 2009Q2 will not now be restored before mid-2014, six years after the recession started. In contrast, the drop in output in the 1930s, which was around 8 per cent, was restored in four years.
The so-called "backcast" to the left of the horizontal line shows the official ONS data. And the fact that the green fan lies close to that line means the MPC doesn't share the views of the "recession deniers" that the latest downturn was a statistical chimera that will eventually be revised away. Such claims, of course, were never credible given that, over the last 10 years, the average quarterly revision by the ONS has been zero.
It is clear from Sir Mervyn King's comments at the press conference that most of the changes to the outlook come from a change in the committee's judgements about the chances of very positive outcomes. Some decline in growth of course comes from the rise in inflation, which acts to depress real wages and hence consumption. One of the major differences, though, compared with the August forecast is that in the new forecast the fan only goes up to 4 per cent, whereas in the previous one it went close to 6 per cent, which I criticised at the time as highly unlikely. In both forecasts the fan stops below at around minus 1 per cent. To quote Sir Mervyn directly from the press conference: "The chances of a rapid recovery are a good deal less than we thought. So in our previous fan charts we did have some probability attached to pretty rapid recovery growth rates of 4 per cent or more. I think we feel that the persistence of the weakness in the world environment has been such now that we attach much less weight to those very rapid growth outcomes; though we don't attach any greater weight to much weaker out-turns". The forecast does certainly look more plausible now. A worry is that it still may be too optimistic.
A big concern is that the Bank provides little detail of its forecasts, and no information at all on the new model it has started using, called Compass. David Stockton in his recent review of the Bank's forecasting performance pointed out: "Compass has now been in active use by the staff as input into the forecast for nearly a year. However, interested parties outside the Bank have little to no understanding of the model and its key features." The Oxford economics professor Simon Wren-Lewis on his Mainly Macro blog* observed that "when monetary and fiscal policy was in the hands of the UK Treasury, the Treasury model was subject to detailed scrutiny". He goes on to conclude that "it does seem regrettable that the Bank has been using a model to produce its forecast for a whole year that no one knows anything about".
An official Bank spokesperson told me: "Spencer Dale set out the Bank's modelling philosophy in a speech in March, and since then Bank staff have given presentations on the new model to colleagues at other central banks and to the IMF. We intend to publish a working paper setting out the model in detail, and give a programme of presentations at UK universities in the new year."
As Fed Governor Janet Yellen made clear this week in an important speech "a growing body of research and experience demonstrates that clear communication is itself a vital tool for increasing the efficacy and reliability of monetary policy". Bank of England take note; the time to be transparent is now.
On the same day as the MPC forecast, the ONS released the labour market data which showed the biggest rise in the claimant count for a year of 10,000 between September and October. The next day we had horrid retail sales numbers. That led David Kern, chief economist at the British Chambers of Commerce, to argue that "GDP will see a sharp slowdown in the fourth quarter".
So despite George Osborne's claim that the economy is healing, we are likely to see three quarters of negative growth, with one Olympics-driven quarter of positive growth followed by another negative one. Indeed, the economy shrank by 0.1 per cent over the last 12 months. Four out of five quarters of negative growth would stand in marked contrast to the US, which has had 13 quarters of positive growth in a row. To rub salt in the wound the credit rating agency Moody's suggested that if this happens the UK would likely lose its AAA credit rating. This represents a major problem for Mr Osborne, who seems to have believed that all would be well in 2015. That dream looks to be in tatters. I am looking forward to the Autumn Statement with much interest.
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|Joerg Heinicke <joerg.heini...@gmx.de>
|Re: [CForms] Support for multipe unique-row-id in Repeater
|Fri, 12 Mar 2004 04:06:33 GMT
On 11.03.2004 17:31, Marc Portier wrote: >> All together we are at: >> >> <fb:repeater id="myRepeaterId" parent-path="." row-path="TheRowPath"> >> <fb:identity> >> <fb:value id="myId1" path="myId1"/> >> <fb:value id="myId2" path="myId2"/> >> </fb:identity> >> <fb:on-bind> >> <fb:value id="field1" path="field1"/> >> <fb:value id="field2" path="field2"/> >> </fb:on-bind> >> </fb:repeater> >> > > yep, sounds like the best we have ATM So, I implemented this myself. To be honest, I'm proud of this work. Not only that I got it working, I also had the feeling I know what I'm doing :) Ok, it took a while to understand all the things in the code, but now it works. I tested it with the form2 binding samples. Some issues: 1. I removed the old attributes and convertor. I will also add this to the woody2cforms upgrade task. Obviously this must by solved by a stylesheet. 2. The repeater binding has to know stuff (fieldId, xpath, convertor, etc.) from its child bindings - and this dependency is bad. With the both attributes all details where known to the repeater binding builder (therefore it implemented the functionality of a second builder) and so to the repeater binding. This is no longer true for the elements. I can get the child bindings from the composed binding, but I had to open (additional getters) the value binding to get the values no longer available to the repeater binding otherwise. This issue goes on: I expect value bindings as child bindings, nothing else (would result in ClassCastException at the moment). I could imagine the use of other bindings here too: the simplest is the context binding, which seems to be absolutely valid, but not working, because there is no chance to get the values from a value binding which is a child of a context binding which is a child of the identity binding. The extreme would be a repeater binding: imagine a list of persons that have a list of biometrical data that constitute the identity of those persons. All together: there is now a dependency of the repeater binding on its child bindings. We can restrict the allowed child elements of fb:identity to fb:value that reduces the problem to current minimum, but maybe something else is needed. >> I read all the threads and use cases. And yes, a unification of the > > pfew, you are a brave man :-) > I need some time to list all changes, proposals, enhancements that were > partially discussed but haven't got into code yet That would be interesting. I guess while reading I lost at least half of the stuff ... >> Also the unification for binding to bean or XML is a one. This ends at >> the latest with the repeater at the moment as the >> @parent-path/@row-path is different for beans and XML. >> > > hm, I've experienced this myself now and then, but I'm afraid this is > out of our league, jxpath imposes an XML way of looking at your > java-bean that is sometimes 'surprising': Yes, I know and fear this. > what most naturally looks like the standard java version of an xml > snippet seems (often due to technical limitations that however logic > need some thought to grasp) to be behaving different in the jxpath view > > next to this observation however I'ld like to question the real-life > relevance: IMHO the advantage of jxpath under the hood of the binding is > that it allows for reusing the syntax-metafor of xpath regardless of the > backend. Being the mix of using slashes over dots, not needing > parentheses and having a single expression that equally works for > getting and setting (LHV/RHV) When it would work "regardless of the backend" ... > I don't see it as a common use case that people during the lifetime > would want to switch the backend from XML to JavaBeans (or vice versa) > and actually expect to have all binding expressions work 'justlikethat'. While developing we often encoupled the backend from the frontend. The interface between both was a simple XML structure. The frontend knows what it will get, the backend what it has to deliver when the system is running. This allows independent development. Additionally we had static test XML files for the frontend, so that "real life" test is possible. The switching was just in the sitemap (XML from disk or from backend). Now I would have to maintain two binding files. Joerg
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Amazon Autocomplete Scraping
- Status Closed
- Budget $10 - $100 USD
- Total Bids 20
Project Description
Needed - a script that makes use of the Amazon autocomplete API to scrape a comprehensive list of keywords from [url removed, login to view]'s search box.
Here's what the script needs to do:
1. Perform for sub-category 'baby' in the search dropdown box.
2. Query the letter 'a' in the search box, scrape and save the 10 autosuggestions.
3. Do the same for b, c, d ... y, z.
4. Do the same for aa, ab, ac ... zy, zz.
5. Repeat steps 1 - 3, except this time don't save the suggestions. Run a filter such that IF the first word of the 10 suggestions is the same for 4 or more of the suggestions, THEN use this as the seed keyword and run 'seed keyword + a', 'seed keyword + b' etc. Save all the results returned.
Duplicates need to be removed.
Important - this is a test project to help me select suitable candidates for a larger Amazon scraping project. I will be selecting multiple candidates for this initial project.
The candidate who impresses me the most will be considered for the larger project.
You must have experience building an advanced scraping application to be considered for the next, larger project.Get free quotes for a project like this
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- Set your budget and the timeframe
- Outline your proposal
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text:
For a really infectious euphoria, there's nothing like a dramatic gesture, a simple act of daring, a demarche, to excite the sympathy. Philip Larkin wrote a poem about the psychology of these gestures, which begins:
Sometimes you hear, fifth-hand,
As epitaph:
He chucked up everything
And just cleared off,
And always the voice will sound
Certain you approve
This audacious, purifying,
Elemental move.
And Larkin agrees. He, too, finds that to hear it said:
He walked out on the whole crowd
Leaves me flushed and stirred,
Like Then she undid her dress
Or Take that you bastard;
Surely I can, if he did?
But of course Larkin ends up unable, taking the longer view, to approve these enviable gestures, just as Auden, in a poem, says that "To throw away the key and walk away" is the kind of action that "makes us well/Without confession of the ill."
Everyone has been describing a kind of euphoria which infected the Major camp as the Prime Minister decided to resign as leader of his party, and they have emphasised the pleasure of Norma at the decision. And this pleasure, this euphoria, spread from the Major family, through his immediate camp, to the party as a whole, to his enemies, his critics, his friends. He did it! He just went and jolly well did it! "Take that, you bastards," he said - or in fact, following verbatim the advice of a leader in last week's Sunday Times - "put up or shut up."
It was a grand gesture, viewed as a gesture. Not as grand as a quick sprint down to Buckingham Palace would have been, with the Cabinet in hot pursuit - not as grand as a resignation as prime minister, dropping the whole Government, in the way one hurries to drop an idea that has proved to be mistaken. This gesture "makes us well", as Auden said, "Without confession of the ill." And so this euphoria had to be short-lived. It will be followed, undoubtedly, by other sensations, other euphorias indeed. But it does not, of itself, solve the problem.
Typical of the euphoria period was the behaviour of Sir Marcus Fox, announcing that the executive of the 1922 Committee was unanimously behind the Prime Minister without, apparently, securing the assent of one David Evans MP, who naturally then decided that the fact of his name being taken in vain was enough to permit him to turn against Major.
Sir Marcus is a man who would clearly prefer the whole of political life to take place under euphoric conditions, with great gestures of unanimity and demonstrations of loyalty to the Beloved Leader.
Typical, too, was John Redwood's initial statement of support for the PM, which served the same purpose as a 24-hour insurance cover slip, now long since out of date. Mr Redwood was not consulted or informed sufficiently in advance. Mr Redwood took to his tent,to sulk like Achilles. Mr Redwood thus became a Trojan stalking-horse, and in next to no time Mr Redwood became the thing itself - this, as Edward Leigh said yesterday, was his moment of destiny. Promoted from being preferable to Norman Lamont, as - let's be plain - cannon-fodder, he suddenly became Mr Preferable-to- Portillo.
How far we are, already, from Larkin's "audacious, purifying, elemental move," this single gesture that will set everything straight. How quickly the argument has been muddied. Edward Leigh tries to make out that the election will not be free, since members of the Cabinet were not free to stand against Mr Major. Stephen Dorrell, in reply, tries to make out that the Cabinet was entirely free to stand, but each member chose, of his own free will, to support the PM instead. Yet a part of the euphoria came from precisely the sense that, by standing for re-election now, Mr Major had indeed forced every member of the Cabinet who wanted to remain in a job to stay super-loyal at least until he, Major, resigned before the second ballot. The Cabinet, after all, is still in office, and must function like a cabinet, to all outward appearances.
Mr Redwood goes missing. The Cabinet Office, we hope, could find him, but Cowley Street, Mr Major's HQ, cannot. But Mr Leigh can find him. He
Half froth, half venom, spits himself
Mr Leigh "refuses to speculate in public" about what he, Leigh, has said to Mr Redwood. But then he goes on and tells us anyway, all the stuff about the moment of destiny.
If the choice was between being a man of destiny and - the more obvious outcome - road-kill, it was worth taking a good weekend to ponder. But as the euphoria recedes and the form of the first ballot encounter comes close to being unveiled, it seems clear to me that the press has already affected the outcome of any ballot by the repeated insistence that, whatever Tory members say about their voting intentions, they must be presumed to be lying.
MPs are as impressionable as anyone else, and if everybody is publicly agreed that they are all lying, then the temptation to lie must surely increase. And if an abstention, or a vote for the stalking-horse/man of destiny, is a more interesting option than a vote for the status quo, well ... you only have to look at these MPs to see that they are the kind who would always pick the scab, even though they know there is going to be blood.
Fifty in opposition on the first ballot is taken as the reasonable, survivable figure; one hundred as being too embarrassing perhaps to survive. But who knows? Perhaps this euphoria is addictive. Perhaps chucking up everything and just clearing off, walking out on the whole crowd, perhaps the temptation to throw away the key and walk away might remain a euphoric option - as Larkin puts it:
Yes, swagger the nut-strewn roads,
Crouch in the fo'c'sle
Stubbly with goodness ...
Perhaps this is the Norma option - give the party one last chance, set up the circumstances in which you would be justified in chucking in your hand. In other words, the mistake is to see John Major as clinging on to power. Rather, he and his wife might be looking for an excuse to swagger the nut-strewn roads.Reuse content


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The first online only game to feature Super Heroes is looking good.
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text:
When you're looking for ideas for decorating with balloons, balloon arches are one of the most popular search returns. Figuring out how to create that balloon arch, however, can be a bigger...view details
With Mother's Day right around the corner, there is no time like the present to take a look at some great Mother's Day gift ideas. While you can search for homeware gifts online that give you some...view details
If you've ever wanted to make your own soap, you might have been worried about having all those essential oils and fragrances on hand. With this homemade coffee soap recipe, you only need a few...view details
If you are looking for creative DIY wine wrap ideas to wrap a gift for wine lovers, then you may want to consider using fabric instead of traditional wrapping paper or a wine bottle bag. Whether...view details
Once you discover how much fun it is to make a flower using a vintage 1970s flower loom you are going to want one for your self.view details
Formal Black Purse, Fabulous Feather Accessory, vintage woven handbag, upcycled with vintage materials and jewelry. Hints of Green and mysterious midnight black fan out beneath the flowing pink...view details
Tribal Feather Necklace, Statement Collar Neck Piece, Brown beaded n feathered, One of a Kind. Vintage rhinestones n tiny silver bells dangle from a vintage hand carved tribal mask. A most unique and...view details
When dressing up for a fun evening on the town with friends, everyone knows it's KEY to wear some BLING and this handmade necklace has both! To learn more about this handmade silver necklace please...view details
FUN - just plain FUN! Handmade Black and White Polka Dot Ring. To learn more about this ring and view more photos, please visit here To view my online shop and see what else I make please visit...view details
This delicate pendant features an asymmetrical pear shape in black and white.It is very light yet very noticeable. The chain dangling from its center add elegance to the piece and contrast the black...view details
I created this lovely purple cotton jersey knit dress in an effort to try to narrow down my sizeable fabric stash. I used the Amazing Fit dress pattern 2404 from Simplicity. It is based on the B...view details
Broken vintage has been given new life in this pretty cocktail ring which features citrus colors all on a gold adjustable ring band. To learn more about this ring or to purchase, please go...view details
This fun handmade ring features an upcycled vintage enamel flower in a lovely shade of purple. The band is adjustable. To learn more about this ring please visit here To learn more about...view details
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Beautiful Polished Pink Quarts & Copper Necklace.view details
Hand painted flower made from permanently stiffened recycled tee shirts.Vintage amber faceted glass bead in center of flower.The base is raw silk plaid in fuchsia, white with aqua and yellow...view details
Mini pillbox hat in cream silk with a sequined band.There is a little gold edging around the top.The hat is held in place with clear elastic that disappears into your hair.Around the face is a...view details
I made this unique silverware jewelry spoon necklace using a vintage silver spoon, an old watch face, and a vintage earring. This pendant hangs from an 18 inch silver box chain. So charming and...view details
This bodycon dress was made as a part of my autumn 2010 basic wardrobe collection. Absolutely neutral it looks absolutely different every time with other items. Unfortunately, these pictures dont...view details
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text:
Recreation of Drawn Image / Logo
This project received 21 bids from talented freelancers with an average bid price of $34 USD.Get free quotes for a project like this
Project BudgetN/A
Total Bids21
Project Description
I have a design (logo if you will) that I recreated from a drawn sketch. The detail in my drawing is a bit rough on the edges, so I need it redone in a large, workable format. ALL of the following versions are required:
1) Illustrator Editable
2) Photoshop Editable
3) Two Angles: a) Looking straight and b) On a slight angle from the side (think flat on the back with a rounded raised front - as if you were to look at different angles of a ring such as [url removed, login to view] - notice the different angle graphics)
4) Metallic/White Gold Version (once again, if you look at the above link, you'll see what I mean by a metallic look)
This is going to be used for a personal piece for someone and it has great sentimental value (please don't copy it for yourself and please do NOT use for a portfolio).
This is a VERY SMALL project - small budget. I would prefer to pay via paypal when all is done as I feel it is safer (Freelancer payment have proven unreliable in the past)
Sample of the file you will be recreating is attached. I can send a larger more prominent one if you are chosen.
Skills Required
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text:
Please review the following admission information and forms before completing the online application.
RN to BSN Application Instructions
BSN Recommendation Waiver Form
BSN Request for Recommendation Form
Online Application—NEW
Please complete your essay and have your unofficial transcripts ready to upload to the application, as it will not let you save and go back to the application.
Application Period for 2017 cohort
December 1, 2016 – March 31, 2017
Application Decision Notification
Applicants will be notified no later than May 15, 2017, of admission to fall, alternate list, or not qualified.
Last Updated December 1, 2016


text:
ObGES Airfield (RU-0109)
Novosibirsk, Russian Federation
Airfields near Novosibirsk
- Yeltsovka Airport UNNE
- Novosibirsk North Airport UNCC
- Tolmachevo Airport UNNT
- Mochische Airfield RU-0110
- Barnaul Airport UNBB
- Lesnoy Airfield RU-0335
- Kemerovo Severny Airport RU-0341
- Kemerovo Airport UNEE
- Spichenkovo Airport UNWW
Airports close to Novosibirsk
Daten und FaktenICAO: RU-0109
Elevation: 0ft MSL or 0m
Exhibition Centres in Novosibirsk
20 Messen
2 hotels from 3 to 4 stars between 15,47 and 27,78 euros at a distance of 14.54 km to 18.71 km.
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River Park Hotel Novosibirsk
14.54 kilometres
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18.71 kilometres
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text:
That Was Then Antiques, Jewelry, and Collectibles
Barbara, Deerfield Beach, FL
These streamlined pieces were made by Aage Weimar from Copenhagen. He worked in the 1937-1966 time period which covers both the leading end of the Art Deco period and the beginning of the Art Moderne, atomic-age period.
The pepper is distinguished by having only one pour hole while the salt has 5. Excellent condition with only the most minor indications of use or age. No dents or dings.
2 other shoppers have this item in their Cart or Wish List .
Unique, Beautiful & Often Quirky - Always Searching for the Best - Especially Affordable Enamels
More than half our inventory now on SALE!!!! Limited time...


text:
Regional news briefs
Relative identifies 4-year-old shot in home
The uncle of the 4-year-old boy who police believe accidentally shot himself identified the child as Keyontist Moffett to WTMJ-TV.
Keyontist was shot about 5 p.m. Wednesday in a residence in the 2900 block of N. 45th St. in the Sherman Park neighborhood. Police believe children were playing with the gun when it fired.
Milwaukee police continue to investigate how children in the home had access to a handgun and will work with the Milwaukee County district attorney's office to determine whether charges are warranted.
A man who lives at the Milwaukee home where the shooting occurred was arrested on suspicion of possessing a firearm as a felon.
Keyontist continues to be treated at Children's Hospital of Wisconsin.
Pilarz named president of Georgetown Prep
Father Scott R. Pilarz, who unexpectedly resigned last fall as president of Marquette University, will start a new job July 1 leading the only Jesuit boarding school in the United States for high school-age boys.
Pilarz will be the next president of Georgetown Preparatory School just north of Bethesda, Md. In addition to being the nation's only Jesuit boarding school, the school also touts on its website that it's the oldest Jesuit school in the U.S., founded in 1789. The school has an enrollment of 490, according to its website.
Pilarz is a professor in the English department at St. Joseph's University in Philadelphia.
Man, 54, found slain
in south side home
A 54-year-old man was found fatally shot Friday evening inside a home on Milwaukee's south side, police said.
The man was found shortly after 7:30 p.m. in the 1500 block of W. Orchard St., and his death is being investigated as a homicide, according to a news release from the Milwaukee Police Department.
The man was pronounced dead at the scene, and no suspects were in custody late Friday, police said.
Barrett urges Walker
to veto early-voting bill
Mayor Tom Barrett and nearly all members of the Common Council are urging Gov. Scott Walker to veto a bill that would curb early voting.
In a letter to Walker, Barrett and all members of the council, except for Ald. Bob Donovan, said they were "dismayed, to say the least, that the State Legislature would support such a blatant attack on voters' rights."
"As elected officials, we should do everything in our power to encourage and promote citizen participation in all elections and unfortunately this bill does quite the opposite," Barrett and council members wrote.
Under the bill, early voting in clerk's offices could take place solely on weekdays from 8 a.m. to 7 p.m. and would be limited to 45 hours in total each week.
In a separate statement issued by Barrett and Common Council President Michael Murphy, the two said Republican legislators "passed self-serving legislation designed to rake in more campaign donations from special-interest lobbyists and make it more difficult for voters in Milwaukee and other larger communities to participate in elections."
"The proposed change to Wisconsin election law is an attack on one of the most fundamental rights of Milwaukee citizens," Barrett and Murphy said.
"If the governor signs this bill, voters in Wisconsin's larger communities will be discouraged from voting and be forced to wait in longer lines to vote early.
"It is wrong to target city voters with unnecessary and punitive restrictions on their right to vote."
UMOS leader urges medals for unit's vets
Lupe Martinez, the president and CEO of UMOS, has added his support to a national campaign to support a bill that would grant the Congressional Gold Medal to veterans of the 65th Infantry Regiment, also known as "The Borinqueneers," originally based on the island of Puerto Rico.
The 65th Infantry Regiment, as mandated by an Act of Congress on March 2, 1899, was the only Hispanic-segregated active-duty unit in the U.S. armed forces and the regiment fought in U.S. wars.
The Congressional Gold Medal has been bestowed on other minority veterans who served in segregated units, including the Navajo Code Talkers, the African-American Tuskegee Airmen, the Japanese-American Nisei and the African-American Montford Point Marines.
In a letter to U.S. Sen. Tammy Baldwin (D-Wis.) asking for support for Senate Bill 1174, Martinez wrote:
"I feel that bestowing the CGM to the Borinqueneers would be a fitting way to honor them as American heroes who served their country with distinction and fought bravely while enduring the hardships of segregation, institutional prejudice, language barriers and other unusual obstacles."
The bill was introduced on June 18 by Sen. Richard Blumenthal (D-Conn.) and seven other original co-sponsors.
The Borinqueneers was the largest, longest-serving and only active-duty segregated Latino military unit in U.S. history, according to information on the website.
With members mostly from Puerto Rico, the unit was active from 1899 to 1959. During the Korean War, 2,771 Borinqueneers received Purple Hearts. About 750 of them were killed in action and more than 120 are still missing in action.
National organizations supporting the drive for a medal include the AARP, League of United Latin American Citizens, the National Guard Association of the United States and the American GI Forum.
Police find 18 pounds
of marijuana in vehicle
A 28-year-old Cedarburg man was arrested Wednesday in Nebraska after the state patrol there seized 18 pounds of marijuana from his pickup, the Omaha World-Herald reported.
Officers stopped Nicholas Pacocha for speeding on I-80 near Lincoln Airport about 11 a.m. A patrol dog picked up the scent of drugs, and the ensuing search yielded three duffel bags of marijuana.
Pacocha was driving 80 mph in a 65-mph zone, the article said.
Dealer sentenced to
12 years in man's death
A Milwaukee man was handed a 12-year prison sentence for selling an Ozaukee County man a deadly dose of heroin and drugs, the Ozaukee Press reports.
Judge Paul Malloy handed the sentence to Mack E. Scott, more than doubling the five-year term recommended by prosecutors in the death of 26-year-old Tyler Bares of Fredonia.
It was the second such sentencing this week in Ozaukee County. Milwaukee heroin dealer Tyler Reddick, 47, was handed a 15-year sentence Wednesday in the 2011 overdose death of Erik Anderson-Yakowicz, 25, of Mequon.
The deaths are linked to a heroin epidemic in suburban Milwaukee.
Teen held in theft
at St. Lawrence
A 15-year-old freshman was arrested in connection with the theft of an undisclosed amount of cash from St. Lawrence Seminary, the Fond du Lac County sheriff's office said Friday.
The teen is being held in the Fond du Lac County Juvenile Detention Center on charges of theft and burglary, according to a news release.
Investigators learned of the missing cash on March 13, and the teen was found in possession of cash consistent with what was discovered missing, authorities said.
A five-alarm fire on March 8 destroyed a section of a building and caused a temporary evacuation on campus.
"The cause of the fire is suspicious in nature and the investigation remains active and ongoing," the release stated.
Anyone with information is asked to contact authorities or call the tip line at (920) 906-7777.
Legislator to run
for secretary of state
State Rep. Garey Bies (R-Sister Bay) announced Friday that he will run for secretary of state.
"The Office of Secretary of State is in the need of a person who can breathe new life into this position," Bies said in a written release. "The current Secretary has held this post for nearly four decades and affairs have slid toward bureaucratic predictability. It's time to apply a fresh and pragmatic perspective to this office."
Current Secretary of State Doug La Follette, a Democrat, has been in office since 1982 and said this week that he plans to run again.
The Associated Press reported that La Follette said he wants to restore the duties that legislators from both parties have taken from the office.
Some Republican lawmakers have proposed eliminating the position entirely.
Walker appoints 3
to Board of Regents
Gov. Scott Walker on Friday appointed three new members to the 18-member University of Wisconsin System Board of Regents, which governs the state's 26 public universities and two-year colleges.
Walker appointed José Delgado, retired president and chief executive officer of American Transmission Co., to a seven-year term, along with Eve Hall, president of the African-American Chamber of Commerce.
Anicka Purath is to serve a one-year term as the board's traditional student representative.
Delgado, who lives in Brookfield, is listed as vice chairman of the Hispanics for School Choice advisory board on the group's website.
Hall, who lives in New Berlin, previously worked for the Family Service of Milwaukee, Milwaukee Public Schools and the Milwaukee office of former Gov. Tommy Thompson.
Purath, a sophomore at UW-La Crosse studying political science, will replace Chad Landes as the traditional student representative on the board. Landes will graduate in May. Purath is from Mount Pleasant.
Illinois educator named UW pharmacy dean
Steven M. Swanson, a professor of pharmacognosy at the University of Illinois at Chicago since 1998, has been named the new dean of the School of Pharmacy at the University of Wisconsin-Madison.
Swanson's leadership, scholarship and energy will play important roles in maintaining and growing the international reputation of the School of Pharmacy, UW-Madison Provost Paul M. DeLuca Jr. said in announcing the appointment Friday.
At UIC, Swanson is associate dean for research and graduate education and visiting vice dean.
He has been a professor of pharmacognosy there since 1998.
In his lab, Swanson studies the effect of dietary substances on the cellular mechanisms at work when cancer begins, as well as the discovery of anti-cancer drugs from natural sources and the role of hormones — particularly growth hormone — in cancer, according to the news release from UW-Madison.
Swanson will begin his new duties July 1. Jeanette Roberts, who came to UW-Madison in 2003, will serve as dean until Swanson arrives and remain a member of the faculty in the school's Pharmaceutical Science Division.
As a dean at UW-Madison, Swanson will serve as the chief academic and executive officer for the college, with responsibility for personnel, budget, fundraising, community relations, curriculum, academic affairs and physical facilities.
Established in 1883, UW-Madison's School of Pharmacy was the first in the United States to offer baccalaureate degrees in pharmacy, and first to grant doctoral degrees in pharmaceutical chemistry, pharmaceutics, the history of pharmacy, social studies of pharmacy and continuing education, and one of the first in pharmacy administration, according to the university.
The school has an enrollment of more than 600 students. It placed fifth among more than 100 programs in the most recent U.S. News rankings.
UW-La Crosse to honor cadaver donors
University of Wisconsin-La Crosse students and faculty will hold an Anatomy Memorial Ceremony on Tuesday to honor those who donated their bodies to science.
UW-La Crosse is one of the few universities in Wisconsin to host human cadavers.
Students of the health professions, biology, and exercise and sports science departments will participate in the 7 p.m. ceremony Tuesday in Valhalla, Cartwright Center-Gunning Addition.
"We are recognizing these individuals for the enormously generous and valuable contribution they have made to our studies and research," student biology department representative Kali Kramolis said in prepared remarks released by the university.
"Most people do not become body donors. It is only a few special few that make this choice," added anatomy senior lecturer Kerrie Hoar. "We honor them through our memorial celebration to let the families of these special people see how much we value this gift."
The memorial, which is open to the public, will include guest speakers from the university, as well as presentations by faculty, staff and students.
From staff and wire reports


text:
Great Sound Quality
Crutchfield Customer Review (What's this?)
Excellent AMP! Replaced my Denon AVR2500 with the NAD -356BEE and very happy with the performance. Connected with BOSE 901-Series VI with active equalizer. Even though my Denon had 85W with THD of 0.05% in comparison with the NAD which has 80 W with THD of 0.007%, can hear the difference in sound quality. Crystal Clear!!!
Pros: Excellent AMP with great sound quality!!!
Cons: None


text: More + Current Season Browse by Play Browse by Range Gifts at the RSC Books DVD & CD Clothing & Accessories Homeware Stationery Prints & Posters The Play's The Thing Food & Drink Toys and Games Matilda The Musical RSC Shop Clothing & Accesories Accessories RSC Logo Key Ring Basket (empty) RSC Logo Key Ring Product code: 50254 Metal key ring featuring the RSC logo with a logo imprint on the reverse. This item is part of a special offer: 2 Shakespeare Key Rings for £5 Buy Any 2 Shakespeare Key Rings for £5 'Though She Be But Little She Is Fierce' Key Ring » RSC Logo Key Ring » 'To Be Or Not To Be' Key Ring » RSC Price: £2.99 Full Members Price: £2.69 Become a member now? How many? 1 2 3 4 5 Add to Basket You may also like... RSC Logo Pin Badge £1.75 RSC More info


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Friday, February 23rd, 2007 by
A Beginner's Guide to Dreamweaver
What is Macromedia Dreamweaver?
Dreamweaver is a WYSIWYG (what you see is what you get) Web editor. It's called that essentially, because everything you see while creating your Web page will appear exactly the same on the Web. In other words, you won't have to fool with messy HTML codes, which can be much more complicated. This software will allow you to quickly code and… Continue reading


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Is Absa Square incorrect or missing key business information? Update your Hotfrog listing today.
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Hotfrog SouthAfrica provides information regarding Absa Square in Mpumalanga. Absa Square is located at Mpumalanga and provides Financial services.
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PO BOX 25 Albertinia Western Cape 6695
2 aalwyn street Albertinia Western Cape 6695
35 NYWERHEIDS AVENUE, ALBERTINIA , WESTERN CAPE Albertinia Western Cape 6695
36 Station Road Albertinia Western Cape 6695
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Kiva Lending Team: Philadelphia Eagles Fans
- About
- Loans 54
- Members 10
- Graphs
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We loan because...
we can
About us
Members of this team support their footbal team through good and bad years. We also support the world community by sharing what we have.
Location: Pennsylvania, USA
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Translation from Arabic to English
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Universal Fit
Astrotec AM90
" I purchased these IEMs after reading about them in ljokerl's Multi IEM review thread. Needless to say, the sound was different than what I expected. Build - These are very, very well built IEMs. Iron housing, good stress relievers, and a cable that flexes with ease. Build was absolutely stunning. Isolation - These isolated very well, at least for me. Never had better..." --pro1137
Scosche IEM856M Reference In-Ear Monitors with tapLINE III Remote & Microphone (White)
"I bought these becuse they seemed high tech and were 75% off. They were strangley not comfertable or user friendly enough for me to put more than 30 hrs on them. Two years later I just pulled them out for comapison reference, and they actually sounded pretty good. They are still fiddley, meaning, I am always aware that they are there, and the bass is meh, ok, and the treble is tiring. I have..." --aafanatic
NEW Astrotec AM800 High Performance In-ear Dynamic Stereo Headphone IEM - Silver
Astrotec AM800 High Performance In-ear Stereo Headphone Specification: Brand: Astrotec Model : AM800 Speaker Diameter: 10mm Dynamic Frequency Response: 8Hz-27000Hz Impedance: 16ohm Sensitivity: 112dB/1mw (S.P.L at 1KHz) Rated Power: 3mW Maximum Power: 10mW Cord: 1.2+/-0.3m PU Special Cable Connector: 3.5mm Stereo Gold-plated Plug
Scosche HP6NC Active Noise Cancelling Ear Buds
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1 Job details Tell us a bit more about the job you need done.
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Sseko Designs - Honey Brown Leather Crossbody Bag
Guide price: $90
Last updated on 4th Jun 2014
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Sseko Designs - Honey Brown Leather Crossbody Bag
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Red Floor Length Anarkali
It's time to turn your glam quotient up with this red raw silk net floor length dress. It features 3 rows with a silk border detailing and unique embroidery on the sleeves and collar.
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Candle Holder
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Last updated on 31st Dec 2010
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Candle Holder
Candle Holder is designed to give an elegant yet classy look to any home decor. It is Crafted to perfection,add color and vibrancy to the ambiance.
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By Jillian Schwartz
Get a chance to learn about a career in health law at Loyola University Chicago's School of Law career panel with health law attorneys and Loyola alumni on Monday, March 31, from 12:00 p.m to 1:00 p.m in Corboy Law Center room 1103.
Here are the full event details from Loyola:
Monday, March 31, 2014, from 12:00 PM – 1:00 PM
- Description
The Health Law Society is sponsoring a career panel. Several health law attorneys and Loyola alumni will be present to speak about their careers. This is a GREAT chance to learn more about different careers in health law, meet health law attorneys, and learn how to be successful in this field. Don't miss out on this exciting opportunity!
- Location
- Corboy Law Center, Room 1103
- Contact
- Melissa Kong firstname.lastname@example.com
- Open To
- This event has been marked as open to the public.
- Sponsor
- Health Law Society


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It's a scenario that would make anyone sweat.
The big-data team at Moz, a marketing software company, was working on a large project to rebuild the systems behind the company's web index for almost two years. Some of Moz's engineers had privately expressed their worries about the progress and lack of results to CEO Sarah Bird.
At any other company, even a private conversation would have been tough, and the whole episode may well have resulted in a failure of the project and a dent in morale. Instead, Moz founder Rand Fishkin says they took the concern public. During a two-hour meeting last week, senior staff were invited to ask the big-data team questions. He tells Fast Company, "It was a great session, and I think everyone walked away feeling more confident in the team and project, as well as more trusting of the individuals involved."
Fishkin says this wasn't a onetime experiment. "We've made it part of our daily processes and part of our manager/employee training to ask for not just honesty, but the whole truth," he says.
The Moz staff isn't alone in their pursuit of honest communications. In fact, a new survey from 15Five indicates that 85% of employees are unsatisfied with the quality of communication in their workplace. According to the findings from over 1,000 full-time employees across the U.S., 81% would rather join a company that values "open communication" than one that offers perks such as top health plans, free food, and gym memberships.
15Five founder David Hassell says that the impetus to do the survey grew out of anecdotal conversations about what really makes great teams and companies. "When it comes down to it, it's the manager/employee relationship," he asserts. Like any good relationship, when there is open communication and trust, a lot of positive things can happen, Hassell says.
15Five had its own dustup over a lack of transparency not long ago. An employee who'd been with the company for almost a year wasn't really up to the tasks she was set to complete. Unfortunately, her previous jobs had taught her not to open up. "It led her to believe it was risky to share weaknesses and safer to put on a front that she had it all under control," Hassell explains. "We only had a candid discussion about this in the final two months of our working together, when it was clear we weren't getting the results we had hoped," he adds. That employee was let go, says Hassell. "I speculate that if she'd been more open earlier on, we may have had a different outcome."
Beyond designated feedback loops, is it possible to instill and enforce a culture of honesty among employees?
Here's a look at several companies who are making it work.
Never Hide Bad News
Rochelle DiRe, chief people officer at Quirky, says that the company's core values were written in the very early days by CEO and founder Ben Kaufman, and transparency was included. Asked if there was a difference between honesty and transparency, DiRe offers: "Transparency is the surfacing of data and events; honesty is about your interpretation and desires. We are committed to being open about both."
That includes never hiding bad news, she explains. "We ask our employees to always state their opinions, even if controversial."
To further encourage honesty and transparency, Kaufman holds all-hands staff meetings every week, and a town-hall meeting with the entire community quarterly. DiRe says, "He will not end the meetings until all questions are answered, whatever they may be. We feel that modeling transparency and openness is what inspires the same in our people."
Form A Culture of Candor Team
At innovation consulting firm Kalypso, Jenna Dudevoir says, "We live by our 'Culture of Candor.'" As a virtual firm, many on its staff of over 200 work together exclusively over the phone, email, and IM from across North America, Europe, and Latin America. "You can imagine the effects that lack of transparency and honesty could have in a virtual business," says Dudevoir, global vice president of marketing.
Dudevoir says that Kalypso first made its Culture of Candor a strategic imperative in 2012 after doubling in size and planning to continue to grow at the same pace.
To keep up with such rapid growth and indoctrinate new employees in the culture, Dudevoir says the company took steps to implement a performance development system, formal counselor-counselee relationships, and a continuous 360 feedback process, including upward feedback for leadership and client feedback, among other initiatives.
The company also designated a Culture of Candor week featuring stories, newsletters, and tips. They also host panels, developed a partner advisory council, and created videos to further encourage conversation around honesty and transparency.
Train to Be More Honest
Joe Silverman, owner of New York Computer Help, an IT computer-service provider, says, "Our motto is total transparency, and I strive to promote this from the staff level through the customer."
Silverman believes that you cannot hide information or be dishonest behind the scenes and still be honest with the customer, and vice versa. If a computer technician started cutting corners, such as not installing all the screws for a MacBook screen replacement, "He would be terminated on the spot," Silverman declares. "It may sound extreme, but if you don't have trust once, you can't expect to have it at all."
Silverman says his staff goes through "excessive training" to promote honesty. One example: "Any time a repair comes in, we ask who hasn't done this before," he says. An honest answer is expected to follow. "Whoever says no gets a crash-test training of how to do it," he says. The result of being honest is helpful training to sharpen necessary skills so no one has to fudge their expertise.
When It's Okay Not To Be Transparent (But Still Be Honest)
Jono Anzalone, a division disaster executive at American Red Cross for 10 regional chapters in 11 Midwestern states, which together have over 116 full-time staff and 5,700 volunteers, adopted 15Five for the employees and managers in his own division, and the tool has spread organically to some other area divisions as well.
Yet Anzalone realizes that the team cannot rely solely on the form. "I often take information learned in my weekly report from my team and follow up with them one-on-one to explore and address root causes."
Anzalone is reluctant to use the word enforce when it comes to honesty. "I would pose that the policy surrounding honesty starts from the one senior leadership sets for the organization," he says. Yet he recognizes that as a leader, there are times when you cannot be 100% transparent, but can still be honest.
"An extreme example is when we are dealing with a disaster survivor's confidential story," he explains. While a volunteer or employee may want to know about the disaster survivor's story, he may not choose to be 100% transparent in order to protect that client. "I can be honest with my employee or volunteer, explaining why I am unable to be 100% transparent," Anzalone says. "A good leader must balance an environment of 'need to know' versus 'nice to know' when dealing with client confidentiality that may impact lives," he adds.