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We describe three extensions of the method of site-specific genomic (SSG) mutagenesis. These three extensions of SSG mutagenesis were used to generate precise insertion, deletion, and allele substitution mutations in the genome of the budding yeast, Saccharomyces cerevisiae. These mutations are termed precise because no attached sequences (e.g., marker genes or recombination sites) are retained once the method is complete. Because the method is PCR-based, neither DNA cloning nor synthesis of long oligonucleotides is required. We demonstrated the efficacy of these methods by deleting an ORF, inserting the tandem affinity purification (TAP) tag, and replacing a wild-type allele with a mutant allele.
In many organisms, it is possible to target mutations to specific sites in the genome. Targeting mutations to the genome allows the effect of specific mutations to be studied in their native genomic context. In particular, native chromosomal DNA may differ from DNA carried on a plasmid with regard to chromatin structure, flanking sequence, and copy number. Each of these features influences the function of chromosomal elements such as promoters, telomeres and replication origins. Thus, targeting mutations directly into the genome has been a valuable tool in many branches of molecular biology.
Several methods for targeted genomic mutagenesis have been developed in the budding yeast, Saccharomyces cerevisiae Targeted mutagenesis is particularly efficient in this yeast because of the high frequency of recombination between chromosomes and exogenous DNA, termed disruption fragments (reviewed in [[]). A disruption fragment can be readily introduced into yeast by transformation [[], and when the ends of this fragment are homologous to genomic sequence, the fragment can integrate into the genome via homologous recombination. Because integration does not require homology in the internal region, targeted mutagenesis can be used to delete and/or insert sequence, depending on the sequence at the ends. Usually the internal region of the disruption fragment contains a "marker" gene, which allows selection for cells that integrate that fragment. For example, a recipient strain containing the ura3 allele fails to grow in the absence of uracil, but transformants that incorporate a disruption fragment containing the URA3 marker can be selected on medium lacking uracil.
Targeted mutations are termed "precise" if the marker gene and other exogenous sequences can be removed, leaving only the mutation. Precise targeted mutagenesis has two advantages. First, it restores the auxotrophy, which can then be used again for a further round of targeted mutagenesis. Second, precise mutagenesis prevents disruption of the targeted region. For example, when an epitope tag is integrated into the N-terminus of an ORF, the marker gene must be removed or it will disrupt transcription from the ORF.
The most common method of precise mutagenesis requires integrating an engineered URA3 allele that can be subsequently removed. This removal can be selected using the drug 5-fluoroorotic acid (5-FOA), since Ura+ but not Ura− isolates are sensitive to this drug [[]. One type of engineered URA3 allele used for this purpose is flanked by direct repeats; homologous recombination between these repeats will "pop-out" the intervening marker [[]. One drawback to this type of engineered URA3 allele is that direct repeats can interfere with the PCR. In order to generate a disruption fragment with a removable URA3 allele completely by PCR, a template containing URA3 allele flanked by E. coli cre-lox recombination sites can be employed [[[]. In this case, expression of the corresponding site-specific recombinase efficiently excises the marker, however the short recombination site remains, meaning that the mutation is not entirely precise.
We recently described a new method for targeting precise point mutations that does not require engineered URA3 alleles and uses PCR fragments rather than cloned genes [[]. This method, termed site-specific genomic (SSG) mutagenesis, involves two steps, and was inspired by an oligonucleotide-based method of precise mutagenesis, delitto perfetto[[]. In the first step of SSG mutagenesis, a PCR fragment is used to insert URA3 near the target site using standard methods (reviewed in [[]). In the second step, a PCR fragment homologous to the region spanning the URA3 insertion is used to replace URA3. This PCR fragment contains a point mutation (or several mutations) at its end. This mutation is present in the PCR fragment because it is contained on one of the primers used to generate the fragment. Thus, at the same time that URA3 marker is replaced, the mutation is introduced into the genome. One advantage of SSG mutagenesis over delitto perfetto is that SSG mutagenesis requires only short oligonucleotides; whereas, delitto perfetto requires long oligonucleotides (80–100 bp), which may sometimes contain unwanted mutations. Furthermore, the region targeted by SSG mutagenesis extends at least 1–2 kb from the marker; whereas, in delitto perfetto this range is only 80–100 bp.
In the present report, we describe several expanded uses of SSG mutagenesis. These methods create precise insertions, deletions and allele substitutions. To demonstrate the first method, insertion SSG (iSSG), a tandem-affinity protein (TAP) tag was inserted at the N-terminus of a protein [[0]. To demonstrate the second method, deletion SSG (dSSG), an ORF was deleted. To demonstrate the third method, allele shuffling (asSSG), a mutant allele from a donor strain was substituted for the wild-type allele in a recipient strain. These methods of precise mutagenesis are rapid and simple, and they have the advantage of not requiring DNA cloning or the synthesis of long oligonucleotides.
Materials and methods
Yeast strains and media
All strains are in the W303 background. Insertions were performed on the strain SH2988 (MAT ade2 can1:ADE2:CAN his3–11, 15 leu2-3, 112 ura3-1). Deletions were performed on the strain SH773 (MATα ade2 can1:ADE2:CAN1 his3-11, 15 leu2-3, 112 trp1-3′Δura3-1). Allele shuffling was performed in the strain SH2867 (MATα ade2 can1:ADE2:CAN1 his3-11, 15 leu2-3, 112 trp1 ura3-1 rme1Δ::LEU2 ime1Δ-lacZ:LEU2). All medium were prepared as described previously [[1].
PCR amplification and mutation analysis
PCRs were performed as described previously [[2]. PCR fragments were purified by Qiaquick columns (Qiagen) prior to transformation or sequencing.
PCR fragments used in Step 1 contained URA3. These fragments were amplified from pRS306 [[3] using 60 nt. primers that contained 40 nt. at the 5′ end homologous to the targeted region and 20 nt. at the 3′ end homologous to the region flanking URA3. In the primers shown below, the 3′ end homologous to the vector are in boldface type. For iSSG, these primers were AAACAGTTTTGCGGTTTCCTTTATACTAAGAAGGTCTATATAACTATGCGGCATCAGAGC and GCCTATTGCTGTCATTGTGGTTGTTGTTATCCTGATCCATCCTGATGCGGTATTTTCTCC. For dSSG, the primers were CGCCAAGGGCTTGTTAATGCGGTGACATGTGTTCTTAGCTTAACTATGCGGCATCAGAGC and ATCCCCCTCGGGTCAATTGATGAAGATTCAGGAGCAGGTACCTGATGCGGTATTTTCTCC. For asSSG, the primers used to construct SH2982 have been described [[].
For step 2 of iSSG, a PCR fragment containing the TAP tag was amplified from pBS1761 [[4] using the primers CAGTTTTGCGGTTTCCTTTATACTAAGAAGGTCTATAGCATACATTATACGAAGTTATCCand GCCTATTGCTGTCATTGTGGTTGTTGTTATCCTGATCC ATCTTATCGTCATCATCAAGTG. For step 2 of dSSG, a PCR fragment was amplified from wild-type genomic DNA using the primers CGCCAAGGGCTTGTTAATGCGGTGACATGTGTTCTTAGCTTACCTGCTCCTGAATCTTC and TGAGGGGAGGATTTGAACTG. For step 2 of asSSG, a PCR fragment was amplified from genomic DNA containing the ime1-sff mutant allele (SH2785) using the primers GTACCCGTACAGCTATCGTTTCAGG and TTTCACCAGTAATGGGTGCCAGTAGG.
Diagnostic PCRs performed on microwaved cells were used to identify transformants containing insertions, deletions or point mutations [[]. To detect insertions and deletions, primers flanked the targeted region. For iSSG, the primers used were pr1 (GGGAAGCCCAAAAATTAAGG) and pr2 (GAATTCGTGGATTTGCTTCC). For dSSG, the flanking primers were pr3 (GCAAAGAAAGGCTTGGAGAG) and pr4 (TGAGGGGAGGATTTGAACTG) and the diagnostic PCR of all strains also contained a third primer, pr5 (CCGGTGTGGGTTTAGATGAC) homologous to URA3. The third primer was necessary because URA3 is approximately the same size as the deleted region. For asSSG, it was necessary to detect the presence of point mutations, so two parallel diagnostic PCRs using the same 3′ primer pr7 (GCTCC CTTTAAAATGCACTACG) were performed. In the first PCR, the final nucleotide of the 5′ primer corresponded to one of two mutations (pr8, AAGTGCGTATCTTTGTGTAA, mutations shown in bold). In the second PCR, the 5′ primer corresponded to the wild-type sequence (pr7, AAGTGCGTATCTTTGTTTAC).
Step one and step two transformations contained approximately 1 μg of PCR fragment and employed a high-efficiency lithium acetate transformation method [[]. Ura+ transformants generated in Step 1 were screened by diagnostic PCR, and at least one isolate containing the correct insertion was then transformed in Step 2. As described previously, in order to select for loss of URA3 in step two, it was necessary to cotransform the PCR fragment with a plasmid containing a second marker gene [[]. In this case, we used 0.3 μg of a 2-μm plasmid containing TRP1 (pTV3), and the transformants were plated on Trp− medium. After 60 h, Trp+ colonies were replica-plated to 5-FOA medium. FOAR colonies appearing after 24–48 h were screened by diagnostic PCR.
Results and discussion
Precise insertions into the genome
One of the most important tools in molecular genetics is the fusion gene, in which the ORF of a gene of interest is fused in frame with either a reporter gene (to measure gene expression) or a tag (to identify or purify the gene product). In order for expression of the fusion allele to accurately reflect expression of the wild-type allele, fusion genes can be constructed at the native genomic locus in budding yeast [[5,–[7]. Because the tag may interfere with the function or stability of the targeted gene, it is useful to construct two alternative fusion genes, one fused at the C-terminus and one fused at the N-terminus. However, when the tag is fused at the N-terminus, marker genes and other intervening sequences must be removed or they will interfere with transcription. Construction of an N-terminal fusion in which intervening sequences were removed (i.e., a precise insertion) was performed using a version of SSG mutagenesis termed insertion SSG (iSSG) mutagenesis. As in the original SSG mutagenesis protocol [[], iSSG mutagenesis involves two sequential steps (Fig. 1A). In the first step, a PCR fragment containing URA3 is inserted into the target region without deleting genomic sequences, and transformants are selected on medium lacking uracil (Ura− medium). In the second step, one of the isolates generated in the first step is transformed with a second PCR fragment, which lacks a marker gene but which contains the insertion sequence. Transformants in which URA3 is replaced with the inserted sequence are selected on medium containing 5-FOA.
iSSG mutagenesis was used to create a precise genomic fusion between the tandem affinity purification (TAP) tag [[0[4] and the GRR1 ORF. In the first step, yeast were transformed with a PCR fragment containing URA3 flanked at either end by 40 bp that was homologous to either side of the N-terminus of the ORF (Fig. 1A) to generate an intermediate strain. In the second step, this intermediate strain was transformed with a PCR fragment that contained the TAP tag and the same 40 bp tails as in the first step.
Transformants from both the first and second steps were identified by diagnostic PCR (Fig. 2A and 2D lanes 2–4). Diagnostic PCR was performed using primers flanking the insertion site. In the first step, among 28 Ura+ transformants, four contained URA3 inserted at the correct site. As demonstrated previously in this strain background [[2], the remaining Ura+ transformants result from conversion of the ura3–1 allele. This conversion may possibly be reduced by substituting the S. cerevisiae URA3 with the partially homologous URA3 gene from K. lactis[[8]. In the second step, 10 FOAR isolates were identified, of which five contained a precise insertion of TAP. The remaining five FOAR isolates likely resulted from gene conversion of URA3 to ura3-1. For two isolates containing a perfect insertion, we verified that the insertion was precise and that no other mutations were introduced by amplifying the region of the insertion by PCR as described [[] and sequencing. In these two isolates and a control strain lacking the tagged gene, protein was extracted, precipitated with calmodulin–sepharose (Amersham), and analyzed by Western blot as described Puig, 2001 #1437. The Western blot revealed a single band corresponding to TAP-Grr1p in both tagged isolates that was absent in the control strain (Fig. 2E).
The frequency of FOAR isolates that contained a deletion of URA3 (50%) was comparable to this frequency in the standard SSG method (60–100%) [[]. We also used iSSG to create a precise N-terminal TAP tag at a second gene, FKH2 (not shown). iSSG has the advantage over previous methods of precise insertion mutagenesis because the tag can be precisely inserted into the genome without expression of exogenous recombinases [[9[0]. However, because tandem repeats of short epitope tags (e.g., 3XHA or 12XMYC) may not amplify efficiently, precise insertion of these tags may be better accomplished by other methods [[6].
Precise deletions of genomic DNA
Deletions of specific genomic regions were created by a version of SSG mutagenesis termed deletion SSG (dSSG) mutagenesis (Fig. 1B). In the first step, the targeted region is replaced with URA3, and transformants are selected on Ura− medium. In the second step, URA3 is deleted from the genome, and transformants selected on 5-FOA medium.
We used dSSG to precisely delete the FKH1 ORF. In the first step, yeast was transformed with a PCR fragment which contained URA3 flanked at either end with 40 bp of sequence corresponding to either end of the FKH1 ORF [[1]. In the second step, the strain generated in step 1 was transformed with a PCR fragment that contained 40 bp flanking one side of the URA3 insertion and 600bp homology flanking the other side. As a result, URA3 was precisely deleted in the second step.
Transformants from both steps of dSSG were screened by diagnostic PCR using primers flanking the target. For the first step, 3 out of 38 Ura+ transformants correctly replaced FKH1 with URA3. For the second step, of 19 FOAR colonies, 3 were shown to have precisely deleted the FKH1 gene (Fig. 2B and 2D, lanes 5–7). To confirm the general applicability of dSSG, we successfully used dSSG to precisely delete a 51 aa domain from an ORF (not shown). One advantage of dSSG over existing methods is that dSSG can be performed on any existing URA3- marked deletion mutant, whereas other methods require first making the deletion using an engineered URA3 and subsequently removing this marker.
Allele shuffling by SSG mutagenesis
Transfer of particular alleles from one strain to another was accomplished by allele-shuffling SSG (asSSG) mutagenesis (Fig. 1C). In the first step, a target gene is replaced by URA3, and transformants are selected on medium lacking uracil to generate a recipient strain. In the second step, URA3 in the recipient strain is replaced by a mutant allele of the target gene, and transformants are selected on 5-FOA medium and screened by diagnostic PCR.
We employed asSSG to transfer an allele of IME1 from a donor to a recipient strain. In the first step, we replaced a 750 bp region of the IME1 promoter with URA3 as in dSSG. In the second step, we transformed this recipient strain with a 1.5 kb fragment amplified from a donor strain. This fragment contained the 750 bp region that was deleted in the recipient strain, 200–600 bp flanking either side of this region and a point mutation present within the 750 bp region in the donor strain.
Incorporation of the mutation into the recipient genome was verified by two parallel diagnostic PCRs (Fig. 2C and 2D). These two PCRs used the same 3′ primer and different 5′ primers. These 5′ primers differed only in their final 3′ nucleotide. In one PCR, this nucleotide corresponded to a mutation; whereas in the other PCR, it corresponded to the wild-type sequence. Of 30 FOAR colonies screened, 8 contained the mutation (27%). Of these isolates, three were selected, and the targeted region was amplified and sequenced in these three isolates. For each of these isolates, we found that the targeted mutation was present and that the remaining sequence was wild-type. Thus asSSG is an effective means of transferring alleles from one strain to another. Note that the same strain can be used as the recipient for many donor alleles.
Advantages of iSSG, dSSG, and asSSG methods for precise genomic mutagenesis
Most methods for targeting mutations into the yeast genome introduce a selectable marker gene together with the mutation. This marker must be removed in order to create precise mutations. As described above, precise insertions, deletions, and allele substitutions can be generated using PCR fragments, rendering DNA cloning and long oligonucleotides unnecessary. The simplicity and efficiency of these methods suggests that they may be applicable in other microorganisms that display high frequencies of recombination directed by short regions of homology.
This work was supported by NIH R01 GM58017 and a grant from the University of Missouri Research Board. We are grateful to B. Seraphin (CNRS, France) for plasmids, and to Mike Carrozza (Stowers Institute, Kansas City) for advice.

subset: commoncrawl


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Personalised Classics - Dracula
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Personalised Classics - Dracula
Become the count! Take the leading role in this classic piece of horror! Choose yourself, family and friends to play six leading characters. Simply substitute the names of the leading characters with your chosen names, using the enclosed cast list to help you. You can even write your own personalised dedication to appear in the front of your book. Once you have decided how you will personalise the book you simply register online or by post.
Your Personalised Classic will then be sent to you. After registration you will be sent your Personalised Classic - first edition. This is dispatched within 28 days of registration. FAQs: How do I register my gift? You can register your gift online or by post. We recommend that you register online as this speeds up the time it takes for you to receive your personalised classic novel.
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Oracles Plans
Like other execs on Tuesday, Robison characterized open source as both a technology and a social movement. The company sells and supports a lot of Red Hat, and he insisted that HP would never build its own flavor of Linux. But he left the door open slightly, saying that if "something fairly disruptive happens," HP would step in and support its customers directly by developing its own product. With so many partners and so little core technology, why buy from HP at all? For accountability, according to Robison. "We will provide a single throat to choke" if something goes wrong, he said. HP still considers its huge Itanium bet a strategic one, especially on the high end, calling it a "very important architecture for us" and "the best competitor for high-end RISC replacement." But Robison was particularly enthusiastic about AMDs 64-bit Opteron, calling it a great architecture. He touted HPs leadership in delivering blades, servers and desktops with the chip, compared with archrival Dell, which has yet to embrace the platform.The day ended with Oracles president, Charles Phillips, as he presented Oracles view of the computing world. With all of the attention on Oracle over the past few months, much of what he had to say had been said before. Phillips did admit that Oracles focus on leading in Linux led to a drop in Windows market share, but he promised that Oracle would be making a big Windows announcement in December to shore up its flagging position. Phillips said he sees Oracles position in the database market as virtually unassailable, mostly due to the companys strong technology talent. "There are maybe 300 people in the world that understand database kernels, and luckily we have half of them." Phillips pooh-poohed the idea that MySQL was any sort of a competitor at all, calling it good for Oracle instead. "Were both in the transportation business," he claimed. "We have a 747, and they have a Toyota." But MySQL and other low-end products certainly have Oracle concerned, because Phillips went on to highlight the $149 Oracle Standard Edition product, the cheapest Oracle ever. Phillips repeated his assertion that the companys push to acquire PeopleSoft is all about increasing customers, and reiterated that Oracle would maintain the PeopleSoft product line for as long as customers wanted both. Read more here about Oracles chances of acquiring PeopleSoft. But when the talk turned to other acquisitions, Phillips was more revealing. "It will get easier going forward," he said, adding that CEOs will be more likely to sell rather than fightbased on the recent court actions in both Delaware and San Francisco. Looking forward, Phillips focused on mobile applications and data broadcasting as exciting areas for Oracle. "Every product will be able to communicate something back about itself," he said. Oracle has built a sensor capture technology to try to capitalize on this nascent market, he said. Check out eWEEK.coms Infrastructure Center at http://infrastructure.eweek.com for the latest news, views and analysis on servers, switches and networking protocols for the enterprise and small businesses.
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- Anemia
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What causes anemia, loss of appetite and nausea? 4 possible conditions
See a list of possible causes in order from the most common to the least.
Celiac disease is a digestive disorder caused by an abnormal immune reaction to gluten. Learn about celiac disease systems, diagnosis, and treatment.
Hookworms are parasites that affect the small intestine and lungs. Read more about the symptoms of this infection and treatment options.
E. coli is a type of bacteria normally found in intestines. But certain kinds of E. coli can cause infection and severe symptoms like diarrhea and dehydration.
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B&B Villa Francesco
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VILLA FRANCESCO - RELAX INSURED The Bed and Breakfast Villa Francesco is a new hotel opened in August 2009 in the countryside iblea 2.5 km from the sea. It offers its guests the opportunity to have a relaxing and comfortable. Francesco Villa is located a short walk from the center of Marina di Ragusa a tourist resort with shops lounge bars and clubs from Punta Secca became famous thanks to the television series Inspector Montalbano and the new tourist port of Marina di Ragusa. The property consists of 4 bedrooms all with private bathroom 1 double and 3 triple: the room RED YELLOW the room the room and the room BLUE ORANGE.


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Here are a few pictures including some of the desserts I provided. The theme revolved around seventies with bohemian and bold accents. I made mushroom sugar cookies decorated in monochromatic neutrals and rounds with edible ink fern transfers. I also did some desserts that channeled the seventies: Brown sugar pound bundt cakes with candied pecans and a modern take on the traditional "Cheerio" Pie. When researching for this shoot, I asked my mom what some popular desserts were in that era, she quickly responded with Cherrio Pie, a cream filled pie topped with cherry pie filling. I thought I would snaz it up a bit with an almond crust and instead of the saccharine sweet pie topping, a fresh black cherry. I was very pleased with the desserts and think they looked fab with the overall styling!
Check out the full post here on The Wedding Post.


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Universal Fit
V-MODA Bass Freq Metal In-Ear Noise-Isolating Headphone (Gunmetal Black)
" Great in-ears. I honestly just typed an entire review on them and my internet crashed. I'll re write it in the future. I will say this though. They NEED a boost from about 200Hz down. Don't be fooled by their name, they lack bass entirely on a flat EQ. That is all " --KodaO
Creative Aurvana In-Ear3
"Creative Aurvana In-Ear 3 Plus Review Hello, my name is Oscar and this is my review of the Creative Aurvana In-Ear 3 Plus. Creative sent these to me as part of a tour and I did not pay for anything, nor am I in any way affiliated with the company other than for this review. I had for a while been wanting to get a listen at these, but unfortunately I wasn't able to purchase them without..." --Fidelity King
Creative Headphones EP-630
" They're a cheap set of IEMs with decent sound for the £10 price. Not much more to say. " --TheJesusGuy
Creative Aurvana 2 In-Ear Headphones
" Comfort and Fit: This pair is very comfortable in the sense that their presence in your ears won't be very noticeable. I fell asleep a few times while listening to music through these earphones, so thats all I can say about them. For fit however, I had to use the largest size of earbuds to fit it into my ears. Isolation isn't very good though because background noise can still be heard even..." --hawk9598
V-MODA Remix Remote In-Ear Noise-Isolating Metal Headphone with 3-Button Apple Control (Rouge)
"V-Moda is well known for having amazing construction while delivering slightly bass heavy audiophile grade headphones, but they also make in ear headphones. And the story is the same with these. The sound signature actually reminded me of a cross between the LPs and the M100s (which I personally own) and just like all of their headphones these offer a relatively balanced sound signature with a..." --gahthatscute
V-Moda Vibe II Earbud Headset with Microphone (Black, iPhone Compatible)
" I owned a pair of these earbuds for the long daily journeys back an forth to University. The come with a selection of different ear fittings and a small leatherette pouch to keep them in. The Good The sound quality of these units isn't bad. It's a little lifeless and flat, but certainly nothing to complain about. The units seem to be very easy to drive,..." --Funkstar De Luxe
VBTO-NERO - Vibrato Sound Isolating In Ear Earphones with Apple 3-Button Remote/Mic - Nero
V-MODA Vibrato Sound Isolating In Ear Earphones with Apple Mic/3-Button Remote Designed for music lovers with style, the Vibrato is sculpted from Zinc Alloy and features next generation hybrid silicone ear-tips. Using unique patented die-casting metal technology that enables consistent sound and unparalleled durability, the Vibrato has a unique geometric design. Crafted from premium metals and...
V-MODA Faders VIP Tuned Metal Earplugs (Gunmetal Black)
Prevent hearing loss! Faders VIP tuned earplugs are essential at clubs, concerts, racing, music practice and events. Constructed from metal for the best sound, Faders VIP reduces harmful noise levels evenly by 12dB. This ensures music and speech passes through with crystal clarity. Faders VIP comes with BLISS hybrid silicone fittings in 4 sizes (XS, S, M, L) enabling a universally tailored fit...
V-MODA Spin IEM's
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V-MODA R-BLUSH Remix In-ear Headphone (Blush)
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One of the side-effects of Britain's otherwise brilliant beer renaissance (see our list of great beer pubs) is that choice of tipple has taken centre stage when choosing a boozer, often trumping less fashionable qualities like atmosphere and heritage. But never fear: for those who like their pint to come with a bit of backstory, there are historic pubs scattered throughout nearby Soho and Covent Garden, and there's a particularly high concentration of them around Fleet Street, on the western fringe of the City. Prepare for tales of bare-knuckle boxing, Dutch sailors and dancing monarchs…
The Lamb and Flag
Now owned by London brewing giant Fullers, this two-floor Covent Garden pub has a long and colourful history – it claims Charles Dickens as a former patron, and was notorious for bare-knuckle boxing in … when it was unappetisingly known as the 'Bucket of Blood'. It's Grade II listed, and although the exterior has been updated the interior still smacks of the 17th Century. There's a nice collection of vintage photos and press cuttings downstairs, and more seating – of the basic, unpadded variety – in the upstairs Dryden Room.
Princess Louise
No-frills Yorkshire-based brewery Samuel Smith divides punters, but one thing everyone agrees on is its commitment to preserving classic pub interiors. The Princess Louise is probably the most impressive of its London pubs – there may be older ones (this is a relative young'un, built in 1872) but there no better examples of a Victorian-era boozer. It's a warren of partitioned spaces surrounding a central bar of elaborately carved dark wood, and the tiling, the mirrors and even the 'facilities' are period. An upstairs room offers much-needed extra seating, but lacks the character of the lower floor.
Ye Olde Cheshire Cheese
Another Samuel Smith haunt, this time on the fringes of the City. But the Cheshire couldn't be more different from the Princess Louise, featured above. Dating back to the 17th Century – the 16th Century original was rebuilt after the Great Fire of London – it occupies a series of gloomily atmospheric chambers off a Fleet Street alleyway. Don't expect much by way of decoration or central heating; exposed brick vaults, wooden benches and open fires are the order of the day. A no-nonsense menu offers big sandwiches and pies.
Ye Olde Mitre Tavern
It may not be the easiest to find, but track down the Mitre and you'll discover the kind of British pub tourists dream of. Cosy and wood-panelled, it's so perfect it regularly features in TV and film productions – look out for it in Guy Ritchie's Snatch. And on sheer age, it takes some beating: originally built in 1546, it claims Elizabeth I among its former patrons – there's even a charming story about her dancing round a cherry tree in the pub's pretty courtyard. With a good range of ales and some hearty pub grub, this is the whole package.
De Hems
From 1685 to 1890 this was the site of the Horse and Dolphin, a coaching inn famous for its association with US slave turned bare-knuckle boxer Bill Richmond (aka 'The Black Terror'). By the turn of the century it had been rebuilt and was being run as an oyster house by a retired Dutch sailor named Papa De Hem – who sowed the seeds of the Low Countries-themed hangout you see today, and gave his name to the establishment in the process. A motley band of poets, gangsters and spies have drunk here over the years, and it was an unofficial headquarters for the Dutch resistance during World War II. A great range of continental brews and lashings of history? Count us in.
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More Videos...
Burts Bandit
all credit goes to the folks at yearone. http://www.yearone.com/yodnn/Portals/yodnn/Portals/yodnn/Home/tabid/54/Defa ult.aspx
Up close and personal with one of our favorite cars. Made popular by Speed Channels latest show R U faster than a Redneck. We present to you...........SUPERCHICKEN!!! CAW-CAW!!!
General lee, starsky , A team and the bandit sunday trip
we took the cars to a show and had lots of fun!!!
Year One Bandit Trans-Am [S2 Ep.1-2]
Quick... what is the most famous car movie of all time? Bullitt? Vanishing point? Gone in 60 seconds? Nice guesses, but in terms of influence and sheer popularity, Smokey and the Bandit stands above them all. Today on GEARZ! Stacey takes a look at not only the cultural impact of Smokey and the Bandit, but also it?s most famous character - the black Trans Am. Then it's off for a test drive of Year One's new Burt Reynolds Bandit edition Trans Am to see if it lives up to the true supercar status of the original. Finally, Stacey takes a look, and shows you how to use, one of the most mysterious and versatile metal working tools out there - the English Wheel. So strap in and hold on, because we're east bound and down...


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Did you know that what you eat can affect your brain's function? The Mediterranean diet and the DASH diet, both known to reduce heart disease, have also been shown to slow cognitive decline.
To look more closely at this association, scientists designed a study called the Mediterranean-DASH diet intervention for neurodegenerative delay (MIND).
As the name suggests, the MIND diet is styled after the Mediterranean and DASH diets but with modifications based on previous research on diet and dementia.
For example, the MIND diet recommends higher levels of vegetables, especially leafy greens, because they appear to have a positive association with slowing cognitive decline. On the other hand, fruits, except for berries, are not associated with slowing cognitive decline.
The MIND diet includes 10 brain-healthy foods:
The MIND diet categorizes five foods as unhealthy:
In this observational study, the MIND diet was strongly associated with slower cognitive decline and had greater estimated effects than either the Mediterranean diet or the DASH diet. Of course, future studies are needed to evaluate whether this diet can prevent dementia.
This news provides yet one more reason to eat your vegetables, especially the green and leafy ones. But what about fruit?
Fruit is a low-calorie, nutrient- and fiber-rich food that has long been a part of a healthy eating pattern. There are still good reasons to include it in your diet. For your brain's sake, consider adding more fresh or frozen berries.
Additionally, let's not rule out all things on the "unhealthy" list. Both the Mediterranean and DASH diets included limited quantities of meat and cheese.
What do you think of this research? What brain-healthy foods will make it on your plate?
Katherine Zeratsky, R.D., L.D.
Selecting "Submit" signifies that you have read and agree to our posting guidelines.
There's many things that your brain needs to be healthy well better start eating right we don't need our brains to stop working there's so many good food's like nuts,frozen berries,green' s ,vegetable's, beans,wheat,seafood,
poultry, when olive oil who would have thought that even wine would be good for your mind!
My neurologist has recommended green tea. Would appreciate knowing about research regarding the benefits.
A r e egg whites okay to eat?
Recently my partner was diagnosed to Crone's disease which is not known in Eastern Asian countries. I combine Maditeranian food approach with Asian way of eating starch grain (rice etc.)combines with leafy greens and soy or meat products. My partner and I are now paying more attention to establishing slow eating habits such as chewing food, paying attention to enjoying the flavor of food mixed with wine, and serving meals with small servings of courses. For example, wine and humas w/whole wheat pita followed by seser salad served with home made dressing -olive oil, anchovies, balsamic vinegar, and main course of blended vegetable soup w/couscous dumpling. These are easy simple everyday recipes. But the key issue is that we are now paying attention to enjoy flavor of food piece by piece, rather than swallowing down a quick sandowitch with everything on it.
This way of eating not only engage mindful eating, but also chewing seems to engage mind integrated in eating. How, more than what, you eat seems to have an impact on brain. Junco Pollack, Atlanta.
What are the one or two published research articles or abstracts concerning this MIND diet? Who are the key researchers involved, and associated with which medical institute?
Why should we eat red meat? there seem to be no advantages to either human health or the environment. Can we not just say NO!
Info and thoughts on resistant starch please.
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Pip Studio Early Bird Mug, Blue
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Last updated on 28th Apr 2015
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Pip Studio Early Bird Mug, Blue
Handmade to give it that special touch, this cute mug is from the PiP Studio collection of tableware. Create an atmosphere where you can share your love, passion and laughter with your dear ones with this beautiful piece of porcelain.
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Calligaris Airport Extending Glass Table with white or neutral glass rectangular top and black nickeled, matt silver, matt black, matt optic white, chromed or satin finished steel legs is an innovative solution for your living or dining rooms. The Calligaris patented telescopic extension system wonderfully doubles the top extension in just one simple step. This extending table's legs get extended with the top remaining at the corners so people can be seated in maximum comfort, the frame being made of metal and extension leaves being made of tempered glass. Extending airport table with black or coffee glass top are also available. Visit
Personalized 9 oz. Rocks Glass – Mr. & Mrs. —
Excellent as table decor or as a parting gift of appreciation, this Mr. & Mrs. rocks glass is an elegant addition to any wedding. Rocking a curly-cue script in a black print, this classic glassware by Kate Aspen can be personalized with names and wedding date—making them custom to you in more ways than one. Features and facts:


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Fidelity promotes Abby Johnson to No. 2
Fidelity Investments has elevated Abigail Johnson to the No. 2 role at the Boston investment giant, reporting to her father, Edward C. "Ned" Johnson III.
The younger Johnson, 50, will now have the added title president of Fidelity Financial Services, "consolidating all of the company's core businesses under her leadership," the company said. The promotion is the clearest sign yet that Abigail Johnson, who has been groomed for years to potentially take over the company, is firmly on target to one day run Fidelity.
She will still report to her father, Edward "Ned" C. Johnson III, 82, who is chief executive and chairman of Fidelity. Other business heads at the company will report to Abigail Johnson.
"During her 24-year career at Fidelity, Abby has gained a breadth of experience overseeing many of the divisions that comprise Financial Services," her father said in a press release. "She has demonstrated an ability to drive change and innovation in business practices on behalf of our customers. She is well suited for this important position."


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Jaina philosophy
From Library of Congress Subject Headings
Jaina philosophy
- http://id.loc.gov/authorities/subjects/sh85100942
- info:lc/authorities/sh85100942
- http://id.loc.gov/authorities/sh85100942#concept
Instance Of
Scheme Membership(s)
Collection Membership(s)
Broader Terms
Narrower Terms
Closely Matching Concepts from Other Schemes
Earlier Established Forms
Philosophy, Jaina
- found: 92-909322: Zaveri, J.S. Microcosmology : atom in the Jain philosophy and ... 1991.
LC Classification
Change Notes
- 1986-02-11: new
- 1993-05-03: revised
Alternate Formats
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The proposed Pediatric Health Services Research Training program is a five-year plan to train Physician Fellows in the problems, methods, and relevant perspectives in child-focused health services research. The application proposes to support 4 post-doctoral Physician Fellows per year, by means of two- or three-year traineeships. The goal of the program is to prepare Physician Fellows for future research careers, through the provision of (a) a solid and multifaceted academic foundation;(b) experience in the conduct of relevant, highly rigorous research;and (c) socialization into the normative culture and expectations of ethically sensitive research conduct and its associated values. To do so, the training program incorporates five core components: coursework, research opportunities, professional socialization, relevant clinical activity, and teaching opportunities. The proposed program builds on the success of the prior ten-year funding period, which represented the first NIH-funded pediatric health services research training program for Physician Fellows. Over these ten years, the program has been notable for the productivity record of the participating Physician Fellows, including first-author publications, extramural funding, and continued involvement in research beyond the fellowship training period, and for program innovations related to formalizing mentor assignment methods;implementation of standardized evaluation tools;institution of a Fellows seminar series;expansion of strategies for recruitment of candidates from underrepresented minority groups;and collaboration with pediatric subspecialty programs on the joint recruitment of fellow candidates interested in combining subspecialty clinical training with health services research training. The primary unit for the proposed program is the Child Health Evaluation and Research (CHEAR) Unit of the Division of General Pediatrics at the University of Michigan;supporting units are the Department of Health Management &Policy, School of Public Health;the Robert Wood Johnson Clinical Scholars Program, School of Medicine;the Michigan Institute for Clinical and Health Research (MICHR);and the Department of Health Behavior &Health Education, School of Public Health. Public Health Relevance: The Pediatric Health Services Research Training Program proposes to continue its successful record in training physician-scientists to conduct rigorous research that addresses the key issues in child health.
|Mueller, Emily L; Sabbatini, Amber; Gebremariam, Achamyeleh et al. (2015) Why pediatric patients with cancer visit the emergency department: United States, 2006-2010. Pediatr Blood Cancer 62:490-5
|Sandberg, Kelly C; Davis, Matthew M; Gebremariam, Achamyeleh et al. (2015) Disproportionate rise in Clostridium difficile-associated hospitalizations among US youth with inflammatory bowel disease, 1997-2011. J Pediatr Gastroenterol Nutr 60:486-92
|Zickafoose, Joseph S; Cohn, Lisa M; Clark, Sarah J (2014) Outpatient utilization by infants auto-assigned to Medicaid managed care plans. Matern Child Health J 18:737-43
|Mueller, Emily L; Park, Elyse R; Davis, Matthew M (2014) What the affordable care act means for survivors of pediatric cancer. J Clin Oncol 32:615-7
|Riebschleger, Meredith P; Van Mater, Heather A; Cohn, Lisa M et al. (2014) Prescription patterns for children with juvenile idiopathic arthritis in Michigan Medicaid: a comparison by prescriber type. Pediatr Rheumatol Online J 12:38
|Sandberg, Kelly C; Davis, Matthew M; Gebremariam, Achamyeleh et al. (2014) Increasing hospitalizations in inflammatory bowel disease among children in the United States, 1988-2011. Inflamm Bowel Dis 20:1754-60
|Hastings, Elizabeth A; Lumeng, Julie C; Clark, Sarah J (2014) Primary care physicians' knowledge of and confidence in their referrals for special education services in 3- to 5-year-old children. Clin Pediatr (Phila) 53:166-72
|Zickafoose, Joseph S; Davis, Matthew M (2013) Medical home disparities are not created equal: differences in the medical home for children from different vulnerable groups. J Health Care Poor Underserved 24:1331-43
|Zickafoose, Joseph S; Clark, Sarah J; Sakshaug, Joseph W et al. (2013) Readiness of primary care practices for medical home certification. Pediatrics 131:473-82
|Zickafoose, Joseph S; DeCamp, Lisa R; Prosser, Lisa A (2013) Association between enhanced access services in pediatric primary care and utilization of emergency departments: a national parent survey. J Pediatr 163:1389-95.e1-6
Showing the most recent 10 out of 44 publications


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Greetings! I am wondering if I could add some new pictures up for the characters. I found some and wondering if I have your permission. Also I am looking for somebody to make a wiki logo custom for me?Loading editor
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Good afternoon. I am the founder and bureaucrat of the Wiki Black Sails Brasil, and I am here waiting for your permission to translate your pages and articles to the Portuguese, so we can expand the culture of Black Sails also to Brazil! Wiki: http://pt-br.blacksails.wikia.com Thanks for the attention and congratulations, great work here on the wiki.Loading editor
I have no objections. You may proceed.Loading editor
Thanks a lot!Loading editor
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Hi im David
I'm new here but i am a huge fan of E.Teach (Blackbeard) The real one ;)
So i was exited to see him in this show and read his informations here in this wiki. But in the trivia informations it is written that his ship the Queen Anne's revenge did not appeared in the show so far. This is wrong. Ep21 minute 54 capt Hornigold passes a Galleon with the flag of Blackbeard himself standing on the ship as well. Please correct this. Maybe some fans are interested in this fact. =)Loading editor
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Aye Carumba! I've missed the entire season more or less! My wife had a baby and I was in the hospital for pneumonia for nearly 3 weeks. Sorry I haven't been any use this season.
Question: did something change on the website where we aren't able to see the numbers of visitors anymore? On the bottom toolbar, there used to be an Admin button that allowed us to see the daily visitors. Do you still have that?Loading editor
No worries, mate. I hope you and your family are in good health.
As far as I know, nothing changed. However, I never actually noticed any number of visitors so I can't tell if it's missing or not. Sorry.Loading editor
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Is Mr. Scott the true father of Eleanor Guthrie?Loading editor
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What makes you think that?Loading editor
In the last episode, when Madi was speaking to her father, Mr. Scott, she said "You have daughters on both sides." That was after she mentioned Eleanor and her rise to power once more through the new govenor. Then Mr. Scott said right before he died, that he only had one daughter, Madi. But you could tell he said it was some contempt and regret.Loading editor
Madi said that because her father had spent most of his time in Nassau taking care of Eleanor. And it was inevitable that, during all those years, some sort of familial bond would would be created. She knew the relationship they had resembled a father/daughter one and that Mr. Scott cared deeply for Eleanor. But in the end, he reassured her that even though he did care for Eleanor, Madi was truly the only daughter he ever had, by saying "Only you".Loading editor
I disagree with that. Why even have that scene if it wasn't a major plot developement. It was all just too coincidental.Loading editor
Seriously is this discussion still going on. Mr Scott is certainly not Eleanor's biological father. He took care of her probably from a baby. Madi and Eleanor used to play together before Madi moved to the Maroons Island. Mr Scott educated Eleanor. It was Mr Scott's rule, not her fathers, that Eleanor could not go down to the beach when she was 13 when Vane first saw her. When her father abandoned Eleanor in Nassau, Mr Scott stayed with her. Eleanor was essentially Mr Scott's adopted daughter. The reason for the scene was to show how much Mr Scott loves Madi.Loading editor
I might be right and I might be wrong. But I felt like it was a valid question.Loading editor
Don't worry, I will not be posting any more questions or opinions on this subject. Right or wrong, I'm entitled to my opinion. Any negativity towards me or my question, was not necessary. I was trying to have an open discussion, which I thought was allowed here. My bad.Loading editor
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Did not know I needed to do that. Anyway: how long did it take for you too realize it was him?Loading editor
Let's keep the MCU Wiki strictly MCU oriented.
Approximately one minute, when I checked the actor's filmography.Loading editor
Okay and okay.Loading editor
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This is probably pointless so no idea why i should try. First sorry to bother you on a different wiki since that seems to annoy the other admins. But i am being treated completely unfairly i was banned tried to talk to an admin on the mcu wiki(there is no way to do this) So i go to their other wiki pages and try and explain my actions and am just repeatedly shut down and then have the ban expanded for contacting them on a different wiki when not only is that the only means of doing so another admin said i HAD to do it that way. I know this is just going to get me in more trouble but it isn't fair! i edited a page twice thats it! I didn't even do it maliciously i explained my self in the publish window confered with people in the comments that agreed posted links to interviews with the information i had then i edited it! Of course i had to be wrong so all the comments i made clarifying with others and posting interviews "mysteriously" disappeared then i get banned for a month! then complaining about it in the only means available(instructed to do so by an admin) just increases it? Why am i being treated as a criminal?Loading editor
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Ahoy, there shipmate! I was wondering if per chance you'd like an article to be done on Henry Avery, a mentioned character in Black Sails. I figured it wouldn't do no harm if this wiki had an article on one of the greatest pirates in history, if not...then I'll understand.Loading editor
I have no objections. In fact, I was also thinking about making articles for the "mentioned only" locations.Loading editor
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First, I should apologize, I have not been here and as an admin I should have kept up with the show and made sure I was here to help.
Second, seeing as I am doing a crap job being here, and this wiki needs to be-able to go on, I figured I should offer you Crat rights should you want them so you can promote new staff as needed without having to either ask me or adopt the wiki and wait a week.Loading editor
What am I, chopped liver? What about me?Loading editor
I thought I got a notice your rights changed. I am glad to see I misread whatever it was I read. I have no reason to deny the same offer to you as well.
While I am still behind, I do care for the show and the wiki, and I know both of you will take care of it..mostly because the two of you HAVE been taking care of it.Loading editor
I won't say no. Thanks.Loading editor
Done, I have also moved myself to inactive. We can always hope I will return, but until then people will know you two are in charge and I am not here. Good work to both of you and let me know if you guys need anything.Loading editor
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Could you ban the person in this thread: http://black-sails.wikia.com/wiki/Thread:5385Loading editor
Even though I did not do the first part from Sannse's advice (the ignoring) I still have to ask you to ban said personLoading editor
That won't be needed sir. Sorry to bring this on your wiki. Uskok, I take my leave.Loading editor
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Kanye Proposes to Kim at a Baseball Stadium. Seriously.
For a guy who seems like he's a romantic at heart, Kanye West sure has a funny way of proposing. In case you missed it, his Yeezusness finally popped the big question to his main squeeze and mother of his child, Kim Kardashian, at, of all places, AT&T Park in San Francisco. Kanye did indeed rent out literally the entire ballpark that houses the San Francisco Giants to ask Kim if she'd like to be Mrs. West. The entire affair took place on her 33rd birthday.
Romantic? Sure. Thoughtful? Absolutely. After all, who rents out an entire stadium in a city where the two don't even reside to propose? There isn't a more scenic venue at this time of the year. Kanye whisked her away to San Francisco and blindfolded her before they headed to the stadium (Kanye is even more genius than originally said because he did it before a gig up by the Bay. Would love to see these reviews).
The couple's friends and family were on hand for the event, which was topped off by fireworks and a 50-piece orchestra, according to E!. It's not like anyone should be surprised, but the orchestra, of course, they played a Kanye song "Knock You Down," in addition to Lana Del Rey's "Young and Beautiful" (which was an interesting choice). Kardashian got a 15-karat Lorraine Schwartz ring. Whatever that means (I know that's A LOT of karats).
But what gets me is that Kanye violated Cool Code 101 when he had the phrase, "PLEEEASE MARRY MEEE!!!" on the stadium's jumbotron. Oy vey. C'mon 'Ye, you could do better than that!
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On one hand, it's super impressive that Kanye--who performs at Honda Center next Friday-- was able to pull off this event without being mobbed by fans or the media (aside from E! of course). But, I never took Kanye as the type to do a ballpark proposal, hell, did anyone? It seems like this proposal, as genuine as it was for the rapper/producer extraordinaire, was just another pawn in the Kardashian game. Just ask that doofus Kris Humphries what its like to get played by them.
Imagine being a fly on the wall to hear what Kanye said to Kris Jenner when he asked for her daughter's hand in marriage. If that did actually happen, don't be surprised to see it on the E! special upcoming for Kanye and Kim: The Wedding (This Time It's Real).
But here's to the happy couple, getting engaged in a grand fashion at one of the best baseball stadiums in the country. I wonder what young North will think of this affair when she is old enough to take in her parents relationship.
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Western Conference Quarterfinals
Game 5
Kings 2, Vancouver Canucks 1 (OT)
(Kings win series, 4-1)
Eight keys to the game
THE FACTS: Down by a goal to start the third period and with the momentum of the series tilting heavily toward Vancouver, the Kings rallied to tie the score Sunday night at Rogers Arena, then won their first playoff series in 11 years on an overtime goal by Jarret Stoll. The Kings will meet the St. Louis Blues in the second round.
THE STAT: The Kings won their fifth consecutive playoff game on the road, including all three in this series, extending their franchise record. It's the first time they've clinched a playoff series on the road since 1993. They also became the 10th No. 8 seed to knock off a No. 1 seed since the playoff format was realigned in the 1993-94 season, and sixth time a President's Trophy winner was eliminated in the first round.
TURNING POINT: Just more than four minutes into overtime, Trevor Lewis poked the puck away from Vancouver defenseman Dan Hamhuis near the Canucks' blue line and Hamhuis tumbled to the ice. As Stoll scooped in the puck and raced off on a 2-on-1, the Vancouver crowd groaned with displeasure, as they thought Hamhuis was tripped. With rookie Dwight King on his right and Canucks defenseman Sami Salo left alone to defend the play, Stoll chose to shoot the puck from the left faceoff circle, beating goalie Cory Schneider in the top left corner for the biggest goal of his career.
HOT: In the days leading up to Game 5, Kings coach Darryl Sutter challenged goalie Jonathan Quick to be as good as Schneider, who had allowed just two goals in both Games 3 and 4 after taking over for Roberto Luongo. Quick met the challenge head on, stopping 26 shots. His best save might have come on a breakaway by Daniel Sedin late in the second period, one that left the door open for the Kings to come back.
NOT: Vancouver forward Ryan Kesler entered the playoffs as the team's third-leading goal scorer during the regular season, but also riding a 12-game goal-less streak. He proved that the slump was still alive and well, going all five playoff games without hitting the back of the net.
GOOD MOVE: Sutter juggled his scoring lines to start the second period, moving Dustin Penner up with Mike Richards and Jeff Carter and dropping King back to the third line with Stoll and Lewis. The move obviously worked, not so much for the Richards line, but it got the third line moving again. The third line ended up scoring four goals in the series, while the second line had one.
BAD MOVE: The Kings went on the power play twice in the first five minutes of the game and managed just three shots on goal, while Vancouver blocked four others. Then it was L.A.'s turn to visit the penalty box, as Drew Doughty was penalized for holding and Brad Richardson took a seat for roughing Chris Tanev. On the second man-advantage, the Kings failed on two chances to clear the puck, allowing Sedinto walk the puck out from the right-wing boards. Sedin faked a shot and got Quick to bite, then centered the puck to his twin brother, Henrik, who scored into the open side of the net for a 1-0 lead, Vancouver's third power-play goal in the past two games. Doughty and Richardson redeemed themselves, however, teaming up on the tying goal in the third period.
NOTABLE: The Kings ended up with 19 blocked shots, including eight for Willie Mitchell and five for Doughty, who also set up the tying goal on a nifty pass to Richardson. Stoll also led the way with a team-high seven hits. The Kings have never won a playoff game against the St. Louis Blues, getting swept in 1969 and 1998. … Schneider was 0-10 in overtime playoff games in his AHL career.
UP NEXT: Game 1 at St. Louis Blues, TBA.


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Packaging Corp. of America's (PKG) $2 billion buyout deal for Boise (BZ) signaled that the M&A wave is now sweeping up firms once reluctant to buy their way to growth, lifting shares of smaller KapStone Paper & Packaging (KS).
Investors were partly applauding the PCA moving off the sidelines to join rivals in making acquisitions. The fifth largest maker of containerboard and corrugated (folded) products said it will pay $12.55 cash per share, or $1.995 billion, including assumption of $714 million in debt, for Boise.
The combined companies generated $5.5 billion sales in the 12 months ended June 30, primarily in packaging, which accounted for 75% of sales and 83% of earnings over the period.
PCA shares were up 7% in eight times usual trading volume in afternoon trading in the stock market today. Shares of KapStone, with about $1.3 billion annual revenue, climbed 8%.
While other companies in the containerboard and packaging industry were expanding via buyouts over the last couple of years, PCA had kept its powder dry while focusing on internal growth.
No. 2 U.S. publicly traded paper and paper products maker Rock-Tenn (RKT), for example, nearly quadrupled its revenue from 2007 to 2012 on mergers and acquisitions. Among key buys, it acquired Smurfit-Stone Container for $3.5 billion in 2011. Rock-Tenn jumped 6% Monday afternoon.
Top paper goods maker International Paper (IP) last year paid $4.5 billion to acquire Temple-Inland. That deal gave the combined company more than one-third of the North American corrugated packaging market. Shares rose 2.2%.
KapStone in July completed its own $1.03 billion acquisition of Longview Paper and Packaging, which included a paper mill and seven box plants.
As part of the latest deal, PCA's total capacity would rise 42% to 3.7 million tons from its current 2.6 million tons. The deal is expected to close in the fourth quarter.
Follow James DeTar at Twitter: @IBD_JDeTar


text:
Disclaimer - I own nothing you may recognize. Typris is mine…that's about it. I hope you enjoy the story! It's my first Narnia Fanfic!
"EDMUND!" Edmund threw his King-sized covers off his head and sat up. He had always thought that if he ignored something long enough, it would eventually go away. Somehow, ignoring Peter never worked.
"WHAT!" He hollered, irritated that he was being awoken in such a fashion. In a second, his giant wooden door slammed open and Peter was standing in his doorway.
"What did you do with my sword?"
"I didn't do anything with it!"
"You had it last!"
"I did not!"
"Where is it then?"
"I just told you I didn't touch it! Maybe you could try listening to me for a change!" Peter silenced. In the few months since their coronation, Peter and Edmund had tried to act more like Kings than brothers, but on occasions such as this morning, Edmund felt as though they were back home in Finchley and he was being yelled at for spilling water all over Peter's homework. Typical. Peter loses something and blames me for it.
"I'm sorry Ed, but I really need to know where it is. I have about fifty counsels set up in the next few months."
"And you need your sword in case someone disagrees with you, is that it?" Edmund knew the remark was childish, but it was too early in the morning for him to be polite. Peter's ears went red.
"It's a symbol of Aslan's blessings upon us and I need it for protection." He said.
"Well, I don't know where it is. Go ask Lucy." Edmund yawned as he lay back down on his feather soft pillow.
"I've already asked her, and don't go back to bed. You've slept in late enough as it is."
"I barely slept in at all." Edmund muttered.
"Nothing, oh High King brother of mine." Edmund replied with a bow. Peter gave him a skeptical glance as he left the room and closed the door behind him. Edmund pulled a face and stuck out his tongue before dragging himself out of bed. "Peter…always blaming me… 'Edmund, my sword is gone. You must have lost it since I'm the great High King Peter and I never lose anything.'" Edmund mocked Peter, lowering his voice as much as he could. "Right."
After breakfast, Peter nearly drove Edmund crazy requesting his aid in finding his sword.
"Why don't you ask Susan and Lucy to help?" Edmund asked, rubbing his head after he had collided rather nastily with a chair while looking to see if Peter had left his sword under the dinner table. "Aren't four sets of eyes more useful than two?"
"I don't want them, especially Lucy, crawling around the floor and getting dust in their lungs."
"But you don't mind if I die from choking on dust. I appreciate that Peter."
"It would be improper to ask them to help."
"Improper? What are you talking about?"
"They're ladies."
"They're not ladies! They're our sisters! They don't care if they get dirty. Lucy used to eat mud, for goodness' sake!"
"This coming from the boy who nearly choked on a beetle means nothing to me."
"I what!"
"Yeah, you tried to eat a beetle when you were two."
"That's disgusting!"
"No kidding."
Edmund was positively appalled. "You never told me about that!"
"You don't remember?"
"No!" Peter laughed. Edmund shuddered. "That is so revolting!"
"Yeah. That was the day you lost your soul to Turkish Delight."
"After Dad pulled that beetle out of your throat you started gagging and Mum felt sorry for you and ran down to the store and bought you a whole box of Turkish Delight. I don't think I'd ever seen you happier than when you took that first bite."
"Well, I think it will be a long time before I can look at another piece of Turkish Delight without feeling sick."
"I bet." Peter laughed, and seconds later Edmund laughed too. Edmund couldn't believe how good it felt to laugh with Peter. He could hardly remember the last time they had laughed together. They were still chuckling when Lucy entered.
"Did you find it?" She asked.
"Not yet."
"Well, Susan sent me to tell you that lunch is being served outside."
"Come on Ed." Peter grinned and offered his hand to help his little brother up. Edmund smiled and accepted his help.
Outside, Susan and Tumnus had laid out a blanket for them to eat lunch on. The four monarchs and the Faun sat and ate and talked and laughed for over an hour.
"…And then the Chief Merman said to the crab, 'Well, maybe you shouldn't be so shellfish." Tumnus laughed at his own joke. Lucy and Susan laughed with him, Peter chuckled, and Edmund smiled politely. He had to admit he had heard better. As Tumnus started another joke, Edmund noticed a large black beetle crawling onto the blanket. Trying not to think about the story Peter had told him, Edmund attempted to brush the beetle off the blanket with his foot.
He missed.
He tried again.
Third time's a charm. Edmund aimed, pulled his foot back, and kicked. "EEEEK!" Oops…
Edmund's foot, instead of kicking the beetle off the blanket, had shattered Susan's wine goblet, sending fragments of glass and wine all over Susan.
"EDMUND!" Peter was on his feet in an instant. Edmund automatically went into defense mode.
"I'm sorry! I didn't mean to…It was an accident!"
Peter gave him a murderous glare before kneeling down beside Susan. "Are you alright Su? Are your eyes alright?"
"I'm fine, Peter." Susan said, wiping wine out of her eyes. Peter gave her a brotherly rub on the shoulder before turning back to Edmund.
"You do realize that you could have blinded her." He accused.
"I wasn't trying to kick her glass!" Edmund could feel his blood boiling. "I was trying to kick that stupid bug off the blanket!"
"Eh-hem." A tiny voice came from the blanket. All four monarchs and Tumnus looked down at the Beetle. "We beetles are actually vastly more intelligent than you larger beings give us credit for."
"Oh…er…sorry…" Edmund said, a bit surprised. He hadn't expected to encounter a talking beetle. Not even in Narnia.
"And do not, for the love of Aslan, call me a bug."
"Right. Sorry."
"Quite alright. Cheerio." The beetle said before crawling off, leaving behind a short silence.
"Ed." Peter said seriously. "Aren't you going to apologize to Susan?"
"I already did!"
"Say it again!"
"Why me? Make that bug apologize to her!"
"Beetle!" The beetle called from the grass. Edmund gave an exasperated cry.
"I can't believe this! Even the insects are scolding me!"
"That's because you can't stay out of trouble." Peter told him.
"Peter, sit down!" Susan said. "It was just an accident."
"That doesn't matter. He'll never learn to control himself." Peter said. Edmund had had enough. With one final icy glare at his older brother, he stood up and stormed off into the woods.
"Good day Your Majesty." Typris, a young gnome whom Edmund had befriended, greeted him. Edmund growled in frustration. "Bad day?" Typris asked.
"You could say that."
"Too bad, Mate. Need to vent?"
"Oh no! Just because Peter thinks he has the right to treat me like vermin just because he's the oldest and I'm his misfit little brother and I get in trouble for everything, even when I didn't do anything…why would I need to vent?" Edmund took a deep breath.
"So Peter's on your case again?"
"Yeah." Edmund said shortly. "You know, he's never been in trouble in his life. He has no idea how hard it is to be in someone else's shadow all the time and to always be scolded by that person. He just makes me so…Urgh!" Edmund was so frustrated, he couldn't finish his sentence. "I'll see you later, Typ." He said as he stomped further into the woods.
Edmund spent the rest of the day in the forest, only returning to Cair Paravel when his hunger outweighed his frustration. He walked silently into the dining hall, glowering at everyone he passed.
"Good evening Ed." Peter said stiffly. Edmund glared at his older brother.
"'Evening." He muttered. Susan and Lucy exchanged glances. The four siblings endured an uncomfortable silence all through dinner. Afterward, they advanced into the living room. Susan picked up a book and began to read by the firelight. Peter taught Lucy to play chess. Edmund sat in a giant armchair and stared at the fire. After an hour, a knock came at the door to the living room. Lucy bounded to open it. A servant stood in the doorway holding a platter of goblets.
"Might I offer your Majesties something to drink?" All four accepted graciously. Edmund noticed that Peter's goblet was gold and decorated with emeralds and slightly larger than the rest of theirs. His, along with Susan's and Lucy's, was silver with tiny sapphires embedded around the bottom. Peter always gets the best of everything.
"This is delicious." Peter said after taking a long drink. "Tell whoever mixed it that they did a spectacular job."
"Yes, your Majesty. I'm sure Typris will be pleased."
"Typris…Isn't he that gnome friend of yours, Ed?" Peter asked.
"Yeah. He loves cooking and mixing stuff like this." Edmund said with a tone that suggested he had yet to forgive Peter for scolding him earlier. He took a sip of the drink and found that it was indeed very delicious. In no time at all, the four goblets were drained. Susan went back to reading her book. Edmund was thinking about going to visit Phillip in the stables when suddenly Lucy cried in triumph, "Ha! I finally beat you!" Edmund was shocked. No one, especially Lucy, could beat Peter at chess.
"You're joking!" Susan cried.
"I'm not! Look!" Lucy pointed to the chessboard excitedly.
"Well I'll be." Susan said, looking impressed. "Well done, Lucy! Peter, I'm surprised at you!" She laughed.
"Peter, are you alright?" Lucy asked, eyeing her eldest brother concernedly. He rubbed his forehead.
"I'm fine…I just feel a little woozy that's all." He said.
"Do you want me to fetch you a warm wash cloth for your head?" Susan asked.
"No…I'm fine." He said as he continued to rub his forehead.
"Perhaps you should go to bed." Lucy suggested.
"That may be a good idea." Peter stood, somewhat shakily. "This just hit me so suddenly."
"You probably stirred up too much dust today looking for your sword." Susan told him."And I know you haven't been sleeping well the past few nights planning all these counsels. You just need a good night's rest."
"I think you're right, Su. I'll see you all tomorrow." As soon as Peter left, Susan looked at Edmund and Lucy.
"I think it'd be a good idea for everyone to go to bed early tonight." She told them.
"Why?" Edmund asked.
"It's been a tiring day." Susan told him. Edmund grumbled incoherently, but hoisted himself off his chair and trudged off to his bedroom.


text:
Aryama C Sundaram
News »latest by relevance
Mar 14, 2016 at 21:05 | Source: CNBC-TV18
Aryama Sundaram and Sanjay Hegde explain if there a legal recourse available for banks and the Enforcement Directorate incase Vujay Mallya does not return to India anytime soon?
Jul 14, 2015 at 10:31 | Source: Moneycontrol.com
Equity Mutual Funds ended positive as the Equity markets in Asia and Europe rallied after the European Union agreed on a bailout package for Greece, subject to conditions.
Mar 26, 2015 at 10:10 | Source: Moneycontrol.com
Mutual Fund NAVs declined as the market remained in a consolidation more on Wednesday ahead of expiry of derivative contracts of March series.
Mar 19, 2015 at 10:10 | Source: Moneycontrol.com
Mutual Funds across categories closed in red as the market closed a rangebound session on a weak note with benchmark indices losing 0.4 percent each ahead of Federal Reserve policy meeting outcome tonight.
Feb 20, 2015 at 15:30 | Source: Moneycontrol.com
Investments in mutual fund linked retirement plans should be considered for income tax deduction under section 80CCD of the Income Tax Act. RGESS may be enhanced and investment limit under section 80C should be increased.
Feb 14, 2015 at 13:53 | Source: Moneycontrol.com
Mutual Funds across categories surge as the market staged spectacular performance on Friday, rising over a percent led by third quarter earnings from SBI and Mahindra & Mahindra.
Jan 01, 2015 at 09:01 | Source: Moneycontrol.com
Sundaram BNP Paribas Mutual Fund A/C Sundaram Growth Fund sold 5,04,656 shares of Shanthi Gears.
Nov 23, 2014 at 14:43 | Source: Moneycontrol.com
Equity and Debt Mutual Funds surge on Friday as the Sensex and Nifty were fired up by a bank-led rally, and ended the week at record levels once again.
Nov 04, 2014 at 10:07 | Source: Moneycontrol.com
On November 03, 2014 Telent Limited sold 3,54,500 shares of TRF at Rs 295.11 on the BSE.
Aug 14, 2014 at 08:33 | Source: CNBC-TV18
ONGC | Tata Steel | Nalco | Voltas | Unitech | Financial Technologies | MCX India | Lanco Infratech | Hindalco | Cipla | Maruti Suzuki | Shree Renuka Sugars | Ashoka Buildcon | KPIT Tech | RCF | Timken India | MMTC | Venus Remedies | BGR Energy | Mercator | Den Networks and Whirlpool are stocks, which are in the news today.
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