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A smooth fit
So far, Oracle has certified the Oracle eBusiness Suite, PeopleSoft, J.D. Edwards and Retek retail application software to work with Fusion Middleware, Kurian said. Oracle has already started the certification process with the I-Flex banking software it acquired in August, Kurian said. "Certainly, Siebel will fit into this architecture very smoothly," he said. In addition, it forms the backbone of grid computing environments and identity management systems, he said. Tod Nielsen, Oracles recently hired senior vice president for systems marketing, said he only decided to leave his position as chief marketing officer at BEA Systems Inc. after being convinced that Oracle is serious about building up its middleware business.Before making a decision to join Oracle, he said he asked probing questions to find out "Does Oracle really care about the middleware or are they just attaching it to the database sales?" He found that year over year since 2001 and through the current year, Oracle has the fastest-growing middleware suite. "The one missing piece has been an awareness in the marketplace that we are players here," Nielsen said. To counter that, Oracle presented two separate panels of corporate customers that are using Fusion Middleware. Major enterprises, including Cisco Systems Inc., ResortQuest Inc. SunGard, SCT and Sumitomo Mitsubishi Silicon USA have implemented Fusion Middleware to integrate key business applications. Cisco is essentially an Oracle-based organization that runs Oracle applications and the relational database in combination with the Fusion middleware Application Server 10g to provide single-sign-on capabilities for all the applications, said David Ott, an infrastructure architect with Cisco, in San Jose, Calif. ResortQuest, a vacation rental service, deployed Fusion Middleware because it used a combination of nearly every Oracle ERP application and database with open-source software such as the Tomcat Java application server from the Apache Software Foundation, said Brian Rasmussen, ResortQuests chief technical architect. The company, Rasmussen said, did an extensive evaluation of the middleware products out there and decided to go with Fusion because "we wanted to make sure we got a single-point solution" that would work with the service-oriented architecture ResortQuest was building into its Web applications. Check out eWEEK.coms for the latest news, reviews and analysis about productivity and business solutions.
Click here to read why analysts suggest Oracle may be more willing to support IBMs WebSphere middleware products in Project Fusion applications.
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November 29, 2012
Today in luxury marketing:
Harper's Bazaar and Hermès team for exclusive sale
For the first time, the French luxury brand will sell its products on a Web site other than its own - the magazine's ecommerce site ShopBazaar.com, WWD reports.
Click here to read the entire story on WWD
Nicolas Ghesquière hangs with the LVMH crowd at Dior dinner
Ghesquière attended a private dinner organized by the LVMH-owned house of Dior, thus rubbing elbows with the luxury conglomerate said to be courting the former Balenciaga designer, per Fashionista.
Click here to read the entire story on Fashionista
Deal Déjà Vu: Ty Warner skips selling Four Seasons New York
Beanie Baby mogul Ty Warner has decided not to sell the Four Seasons Hotel New York, even though he had offers of roughly $900 million for the luxury property in Midtown Manhattan, according to The Wall Street Journal.
Click here to read the entire story on The Wall Street Journal
Neiman Marcus fiscal 1Q operating profit up
Neiman Marcus Inc.'s fiscal first-quarter net income rose 4 percent, helped by higher sales at existing stores, Bloomgberg Businessweek reports.
Click here to read the entire story on Bloomgberg Businessweek
Like this article? Sign up for a free subscription to Luxury Daily's must-read newsletters. Click here!

subset: commoncrawl


text:
Skip to Main Navigation. Each navigation link will open a list of sub navigation links.
Outreach Credit Programs
1000 E. University Ave., Dept. 3274
Laramie, WY 82071
Phone: (307) 766-4300
Toll Free: (800) 448-7801
Email: firstname.lastname@example.com
We recommend Audacity for the purposes of creating and editing audio files. It is free, open source, cross-platform and extremely easy. The information below should help you work your way through the installation and use of Audacity. Please contact us if you have any questions.
You need to install Audacity for the purposes of editing audio files. The LAME MP3 Encoder must be installed to export your audio file as the recommended MP3 file for distribution.


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215 Rice Athletes Named To C-USA Commissioner's Honor Roll
Rice Ranks Third In C-USA
JUST FOR KICKS - Meet The Rice Soccer Team!
Rachel Petmecky
Rice Tops C-USA with 232 on C-USA Commissioner's Honor Roll
Conference USA Commissioner's Honor Roll
Owls Meet SMU Wednesday In C-USA First Round Action
Start Time With Mustangs Set For 2:30 PM
Owls Play C-USA Games At Collorado College, UTEP, This Weekend
Dynamic young player who is versatile enough to be on the backline or in the midfield... Powerful, agile and feisty, and absolutely loves to play the game... Makes incredible sideline throws.
2011: Conference USA Academic Honor Roll; Played in all 21 games with 16 starts on the backline... Helped the Owls' hold opponents to just 11.4 shots per game, the lowest total by the program in four years... Averaged 75 minutes per start and totaled a new career-high of 1,361 minutes overall... Played 90 or more minutes five times, including 108 minutes in the home victory over LSU... Her tackle against LSU in front of the goal at the 18-yard line on a breakaway in the closing seconds of regulation averted what would have been an open shot, and helped force overtime (where the Owls then pulled out a victory 18 minutes later)... Made the long throw-in to get first collegiate assist on Ashton Geisendorf's game-winning goal against longtime rival SMU in the opening game of the Conference USA Tournament... Came forward to take four shots, including two in the home win over McNeese State
2010: Earned the program's Most Improved award... Stepped-in to start the final eight matches of the year on defense and was a major contributor to the four shutouts the Owls posted in the final month of the season... Finished the year with 825 total minutes, with close to 80 percent of her total playing time amassed in her eight starting assignments... Averaged 82 minutes per start... Played a career-long 110 minutes in the shutout of crosstown rival Houston... Came forward to take nine shots, including four on-goal... Maintained a .444 shots-on-goal percentage.
Texas ODP 2006-2008... Club standout for AHFC `93 Blue under head coach Danny Hill... Gelped club to two state finals and one third place finish... Semifinalist at Disney Showcase... Served as the club's team captain.
High School
A 2010 graduate of William B. Travis High School in Fort Bend, Tex... Versatile athlete who also played varsity volleyball and basketball... Coached in soccer by Melissa Fox... Two-time first team all-District 23-5A... Earned the Tiger's Offensive MVP as a senior in 2010 and junior in 2009... Named the 2008 District 23-5A Newcomer of the Year... Served as the squad's team captain..
Full Name: Rachel Marie Petmecky. Born: October 7, 1992, in Houston. Parents: Robert Kathy Petmecky of Sugar Land, Tex. (one brother). College: McMurtry.
|McMurtry College
More Headlines
Fichera & Chaiken Lead Six Owls In C-USA Soccer...
11/01/2016 - On the eve of competing in the annual Conference USA Soccer Championship, the Rice duo of Samantha Chaiken and Jenny Fichera led a total of six...
Rice Tops FAU In Overtime For 7th-Straight Win
10/28/2016 - Sophomore Annie Walker scored the game-winning goal in overtime to lift the red-hot Rice soccer team, now winners of a school record-tying seven-st...
Soccer Closes Out Regular Season Friday At FAU
10/25/2016 - The Rice Soccer team wraps-up the 2016 regular season schedule on Friday (Oct. 28). The Owls travel to Boca Raton, Fla., to face Conference USA foe...
Isokpunwu Named C-USA Soccer Defender of the Week
10/24/2016 - Senior Jasmine Isokpunwu has been named the Conference USA Soccer Defensive Player of the Week, the league office announced from its headquarters...
Rice Blanks Charlotte 2-0 For Sixth-Straight Win
10/23/2016 - Goals by Marissa Topolski and Jasmine Isokpunwu paced the Rice soccer team to its sixth win in a row with a 2-0 Conference USA shutout victory over...
Owls Win Fifth-Straight, Punch Ticket To C-USA ...
10/21/2016 - Second half goals by Aliza Wolfe and Nia Stallings helped lift the Rice soccer team to a 2-0 Conference USA shutout victory at home Friday night at...
Owls Host Crucial C-USA Matches This Weekend
10/19/2016 - For the first time in three weeks the Rice Soccer team is back home for Conference USA league matches.
Soccer Wins 4th-Straight; Claims Share of 1st...
10/14/2016 - Junior forward Nia Stallings assisted on the first goal of the game and then scored the match-winner with less than five minutes left in regulation...
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The Wave Vol 4: Through a Glass Darkly now available on Kindle!
Eczema can absolutely, totally and completely be cured by diet (as can pretty much everything else). A zero gluten, zero dairy and zero sugar diet with very low carbs (less than 40 a day) and high quality animal fat and protein will make it all literally disappear.Cassandra, if you're that chemically sensitive it also sounds like you need to get some strong detox protocols going. There is a lot of information on that on the forum, if you're interested.
Of course, my flare ups could also be cause by my occasional veering of the diet when there's nothing else to eat or my "cheats",
A zero gluten, zero dairy and zero sugar diet with very low carbs (less than 40 a day) and high quality animal fat and protein will make it all literally disappear.
I also found it very helpful for Folliculitis and dandruff that I occasionally get on my scalp. After I wash my hair, I rinse with diluted ACV. Granted, I don't do it as much as I like, cuz my kids complain I smell like a pickle, but the smell does go away once completely dry.
Funny thing was, that my eczema got much worse when I gave up smoking for two years. That's the time I had to take painkillers, so I could get some sleep, which the itchiness prevented.
I've used ACV to help relieve the pain of insect bites, so I know it does have some positive effects of relieving inflammation of the skin. But for chronic conditions like eczema, I would try to focus more on diet, fwiw.
I would try to focus more on diet, fwiw.
My grandmother used ACV on us for any type of skin problem and in particular sunburns... it works so well... The vinegar evaporates quickly taking a lot of the "heat" out of the burn... It also helps heal quickly but I have no idea why!


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Preventing fraud
We have a zero tolerance policy to fraud in all its form including bribery, corruption and theft.
Our approach to managing fraud and corruption is set out in the following documents:
Fraud updates
- 21 people were employed to investigate fraud in 2015-16
- we did not employ any professional accredited counter fraud specialists
- the total cost of employee time spent investigating fraud was £43,087
- 40 fraud cases were investigated.
- 25 people were employed to investigate fraud in 2014-15
- we did not employ any professional accredited counter fraud specialists
- the total cost of employee time spent investigating fraud was £44,168
- 28 fraud cases were investigated.
National fraud initiative
This authority is required by law to protect the public funds it administers. It may share information provided to it with other bodies responsible for auditing, or administering public funds, or where undertaking a public function, in order to prevent and detect fraud.
The Cabinet Office is responsible for carrying out data matching exercises.
Data matching involves comparing computer records held by one body against other computer records held by the same or another body to see how far they match. This is usually personal information. Computerised data matching allows potentially fraudulent claims and payments to be identified. Where a match is found it may indicate that there is an inconsistency which requires further investigation. No assumption can be made as to whether there is fraud, error or other explanation until an investigation is carried out.
We participate in the Cabinet Office's National Fraud Initiative: a data matching exercise to assist in the prevention and detection of fraud. We are required to provide particular sets of data to the Minister for the Cabinet Office for matching for each exercise, as detailed on the Gov.uk website.
The use of data by the Cabinet Office in a data matching exercise is carried out with statutory authority under Part 6 of the Local Audit and Accountability Act 2014. It does not require the consent of the individuals concerned under the Data Protection Act 1998.
Data matching by the Cabinet Office is subject to a Code of Practice.
View further information on the Cabinet Office's legal powers and the reasons why it matches particular information. For further information on data matching at this authority, please contact the Internal Audit team on 0115 9772240.


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This pump is the perfect accessory to any 1000ml Redken Shampoo or Conditioner, making your large bottles easier to handle. The 1000ml pump dispenses the conditioner or shampoo with ease.
Thank you for visiting Redken Pump 1000ml on seekdb.com
We bring the products from many websites to one website for you to search, save and compare.


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PetSafe Extra Wireless Fence Receiver Collar
Online Item #: 0000000000871
- For dogs 8 lbs. & up, fits neck sizes 6 to 28 in.
- Double the pups means double the love and double the trouble!
- Make sure both of your family's 4-footers stay exactly where they're supposed to
- This extra receiver collar can be used to add another dog or as a replacement collar to the Wireless Fence Containment System, and the Stay + Play Wireless Fence
- 5 adjustable levels of correction plus tone only mode
- Low battery indicator
- Waterproof collar is submersible up to 5 ft.
- Includes battery
- Weight: Approximately: .45 pounds
- Shipping Dimensions: Approximately 9 x 6 x 3 inches.
Tab 4 - Future Phase
Prefer to place your order by phone?
Reach us at 877-633-7456 M-F 7:00am - 6:00pm Central Standard Time.
Reach us at 877-633-7456 M-F 7:00am - 6:00pm Central Standard Time.


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Jenny McCarthy Offered to Star on 'Real Housewives of New York'
- Thu Aug 20, 2015, 8:32 AM GMT
The former 'The View' co-host reveals she's approached by Bethenny Frankel to join the cast of the Bravo reality series.READ MORE
The former 'The View' co-host reveals she's approached by Bethenny Frankel to join the cast of the Bravo reality series.READ MORE
LuAnn de Lesseps tweets to Bethenny, 'Welcome back,' while Sonja Morgan says she's 'very excited' after learning that Bethenny will rejoin the cast of the Bravo reality show.READ MORE
Frankel says she's visiting Bravo's headquarters in New York City to talk with Andy Cohen 'about two different projects.'READ MORE
Bravo reportedly fired Drescher and demoted Singer to a recurring role because they want to build the reality show around cast members Heather Thompson, Kristen Taekman and Carole Radziwill.READ MORE
Drescher says she doesn't 'have any regrets at all' for throwing her prosthetic leg, claiming that she only did it because her co-stars 'didn't want to hear what I had to say.'READ MORE
Drama will unfold in the fifth season as Ramona Singer has a problem with both her old friends and the new ones.READ MORE
Jill Zarin, Kelly Bensimon and Alex McCord will reportedly be replaced in season 5 of 'The Real Housewives of New York City', but both the network and the stars' camps deny the story.READ MORE
Kristin Cavallari is ready to dance in a tight mini dress, Chaz Bono looks dapper in a vest and white shirt, while David Arquette looks sharp in a tux.READ MORE
A backstage fallout between Ramona Singer and one of Shed Media staff was allegedly not part of the show.READ MORE
While Alex McCord and Kelly Bensimon were partying at Pierre Hotel, thieves broke into the crew's van and took $30,000 worth of equipments.READ MORE
The owner of Completely Bare spa, Cindy Barshop, reportedly is the replacement for Bethenny Frankel who left last season and would only appear occasionally in season 4.READ MORE
Bethenny Frankle juggles her new life as a wife and a mother to a baby girl in the show which requires no yelling in order to be entertaining.READ MORE


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# of Nights: # of Rooms: 1
My Reservation Best Rate Guarantee >
Book us direct for our Best Rates - Guaranteed!We are confident our best rates can only be found here on warwickhotels.com. View Details
Beach side, Jiyeh, Lebanon - Reservations +961 7 995 580 - firstname.lastname@example.com
Elegance and modern amenities permeate throughout each of the 63 rooms and 20 bungalows at Warwick Pangea Beach Resort & Spa in...
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While some say Warwick Pangea Beach Resort & Spa is best suited for relaxation, others vouch that its true calling is as host to...
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Photo Gallery
A picture paints a thousand words. Warwick Pangea Beach offers the following photo gallery to get the conversation flowing.
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Explore Beirut
Throughout history, Beirut has always welcomed people searching for a better life.
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A Mediterranean stay would not be complete without experiencing and enjoying cuisine indicative of the local culture. And at...
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Information: Please note that Warwick Pangea Beach Resort & Spa is closed for the end of season from October 16th 2016, until May 15th 2017.
To find an alternative accommodation, please visit our Warwick Palm Beach website.
The essence of much of the Mediterranean coast is how natural beauty and unending comfort blends effortlessly. But it is at Warwick Pangea Beach Resort & Spa where this blend of luxuries is perfected. Starting with its exclusive location near the quiet, soft shores of Jiyeh, breathtaking views prime guests for a memorable stay. While the location creates a scenic backdrop of beauty, it is the elegant details inside the resort and along the seaside shore that make it worthy of even the most sophisticated world traveller. The local flavours tasted at the Waterfront Lebanese and Seafood Restaurant, the refined Mediterranean styling in every room and the privacy offered at the Bungalows positioned poolside and above – it is all meant to create the most desirable accommodations possible. And luckily for guests, it does just that. Welcome to Warwick Pangea Beach Resort & Spa.
Welcome Home, Warwick Journeys Member. Designed for convenience this website allows you to manage your profile and preferences, gain access to member exclusive offers and benefits, and manage your reservations booked on WarwickHotels.com.
Please agree to the terms & conditions to continue.


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Primus® OmniFuel Stove w/ 0.6 - liter Fuel Bottle
Item # WX2-137612
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- Details & Specs
Primus® OmniFuelTM Stove gets you cooking faster and more efficiently!
The tried and true OmniFuelTM uses very advanced materials to offer advanced functionality and is designed to handle demanding conditions, extreme altitudes and both high and low temperatures.
OmniFuel is a sturdy Stove that works with LP gas, gasoline/petrol, diesel and kerosene - even aviation fuel. Using the separate control knob, the flame can be adjusted to provide the exact heat required. The ErgoPumpTM fuel pump, a multi-tool with an integrated cleaning needle, nylon stuff sack and windscreen / heat reflector are included, too!The rest:
- Boils water in 3 minutes
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- 10,500 BTU/h
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Get yours ONLINE today!
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How to calculate Dow Jones Exponential Moving Average - illustrated with data in excel spreadsheet.
What is exponential moving average(ema) and how is it different from simple moving average(sma)? Simple moving average takes an average of the no of days. So a 20 day sma would calculate an average of all the figures for preceding 20 days. In EMA, we give more weight to recent observations. This is done via using a factor called exponent.
The value for the exponent = 2/(No of days +1)
So for EMA of 20 days the exponent = .047619
Now we multiply today's closing figure with this exponent, and yesterday's EMA with (1-exponent) to get new ema. Obviously you have to start somewhere, so we start with a simple average for the first 20 data points and then move onto the EMA formula from there. Confusing? Have a look at the excel sheet it will become a little more clearer as to what it is that I am talking about and how exactly it is calculated.
Label: ema excel
Label: ema excel


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Greenspec Solutions
Construction Materials, Construction, Architects
Kingspan Kooltherm® K18 Insulated Plasterboard is a super high performance, fibre-free rigid thermoset modified resin insulation, sandwiched between a front facing of tapered edge gypsum based plasterboard, and a reverse facing of low emissivity foil autohesively bonded to the insulation core during manufacture.Kingspan Kooltherm® K18 Insulated Plasterboard is manufactured without the use of CFCs/HCFCs and has zero Ozone Depletion Potential (ODP).
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• Reduces noise breakout from pipes. • Unique flexibility of the polymer-based noise barrier provides superior performance and allows even the smallest pipes to be lagged effectively • Independently tested in laboratory conditions and in situ to give proven consistent performance • Leading consultants specify Soundlag 4525C with confidence • High performance noise insulation with good low and medium frequency transmission loss
Silentstep is a cost effective high performance acoustic underlay that offers excellent support for all types of carpet. Silentstep provides a significant reduction in both airborne and impact noise from the floor above into the room directly below in two storey domestic and commercial applications. Silentstep works to control footfall noise (impact) in inter-tenant living, reducing airborne noise from radio, TV, home entertainment systems or human voice.
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Greenspec Solutions supports our Thermal and Moisture control range of products with a computer based service, designed to provide fast and accurate technical advice. Calculations can be carried out to provide R-Values, Condensation/Dew point risk analysis, and required Insulation thicknesses to meet Building Code regulations. Achieve your Targets – Satisfy Building Compliance – Talk to Greenspec Solutions TODAY
● Super high performance rigid thermoset modified resin insulation ● Fibre-free insulation core ● 3-in-1 insulation, dry-lining and vapour control ● Group 1 BCA fire classification ● Energy saving - allows quick response heating and cooling ● Clear cavity is maintained - resists moisture penetration ● Resistant to the passage of water vapour ● Easy to handle and install ● Ideal for new build and refurbishment ● CFC/HCFC-free with zero Ozone Depletion Potential (ODP) ● Compliant
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More than a year after the last tenants moved out, the Professional Arts Building at 121 W. Locust St., Davenport, is coming down, likely to be replaced by a bank and a restaurant.
Although tenants had to be out of the building by Nov. 1, 2011, asbestos removal and utility issues slowed down the demolition, developer Ted Rebitzer said.
City officials have said there have been discussions for a McDonald's restaurant at the location, but Rebitzer wouldn't identify potential tenants of the 1.5-acre site, bordered on three sides by Brady, Main and Locust streets.
"We'll wrap up demolition in the next couple of weeks and hopefully have an announcement shortly," the developer said.
Rebitzer and business partner Andy Doyle plan to divide the parcel for the tenants. Building design will have to go to the city's Design Review Board for approval.
Main at Locust Pharmacy, 129 W. Locust St., is attached to the three-story Professional Arts Building, which will remain at the intersection of its namesake streets. Although the two buildings are attached, the real estate parcels are separate.
During asbestos removal, an unmarked underground electrical line was discovered that needed to be relocated, causing a significant part of the delay.
"We didn't find out about the electricity running through the property until then," Rebitzer said. "We did our due diligence, but it was an unfiled easement."
Construction workers also found telephone lines to the building cross-wired that required relocating.
Rebitzer and Doyle want to get demolition completed by January so the building can be removed from the tax rolls.


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Forest Connect
To restore forests and get out of poverty, rural communities need the knowledge and connections to build flourishing enterprises. Forest Connect aims to reduce poverty and protect forests by better linking locally-controlled forest and farm enterprises, not only to each other, but also to markets, financial and business support services and to decision makers, policymakers and policy processes, such as National Forest Programmes.
Creating these connections has had some important results. In Nepal, for example, forest communities were recently linked into a supply chain that built better charcoal briquettes and stoves, cornering the market for fuel in Kathmandu. Read more about this.
Forest Connect catalyses practical support for locally-controlled forest enterprises. An example includes this Facilitator's toolkit for supporting small forest enterprises.
It has also facilitated the sharing of learning and exchanges between partner institutions.
How it works
Forest Connect has an international steering committee, and is co-managed by IIED, UN's Food and Agriculture Organization and The Center for People and Forests (RECOFTC).
Forest Connect is often referred to as an ad hoc alliance because it is made up of individuals and institutions, all of which are committed to supporting locally-controlled forest enterprises and who share experiences and learn from each other, but not through a formal membership structure. Central to this way of working is a social networking platform, which currently involves more than 1,000 individual members in 94 countries.
At the national level, Forest Connect comprises national institutional 'hubs', some of which have received modest financial support from international partners, but all of whom source and use their own resources in support of locally-controlled forest enterprises. There are active programmes in Brazil, Cambodia, Democratic Republic of Congo, Ethiopia, Ghana, Guatemala, Guyana, Indonesia, Laos, Liberia, Mali, Mexico, Myanmar, Nepal, Thailand, Uganda and Vietnam.
In addition, IIED supports more in-depth analysis of options for investing in locally-controlled forestry in Ghana, Guyana, Mozambique and Myanmar. In each country, we try to ensure that locally-controlled forestry is recognised and supported in key policies concerning sustainable forest management, the legal timber trade, climate change and integrated land-use planning.
At an international level, we gather evidence of what works (and what doesn't), organise international learning events and platforms to share such information, distil findings into briefing materials, and pursue investment in support of locally-controlled forestry.
This work is currently funded by the UK Department for International Development.
Forest & Farm Facility hosted by the FAO
The Center for People and Forests (RECOFTC)
Brazil: Instituto de Pesquisa Ambiental da Amazonia
Burkina Faso: TreeAid
China: Forest Policy and Information Institute of the Chinese Academy of Forestry
DRC: Tropenbos International DRC
Ethiopia: Farm Africa
Ghana: Tropenbos International Ghana
Guatemala: Utz Che
Guyana: North Rupununi District Development Board
Liberia: Save my Future – SAMFU Foundation
Mali: Association des Organisations Professionnelles Paysannes
Mexico: Reforestamos Mexico
Myanmar: Pyoe Pin
Nepal: Asia Network for Sustainable Agriculture and Bioresources
South East Asia (Cambodia, Indonesia, Laos, Thailand, Vietnam): The Center for People and Forests (RECOFTC)
Uganda: Natural Enterprises Development Ltd
Locally-controlled forestry
Supporting locally-controlled forestry for improved livelihoods, justice and sustainability


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"There's basics the guys have to get done, like they have to be strong, they have to be able to squat and we've got benchmarks there but the trick is when they get there how do we use that strength or how do we use that conditioning.
That's more so important during the season because we are able to manipulate training sessions where we can take some of the stuff we're using in the wrestling and we can incorporate that into skills sessions, like Axel with his breakdown work.
It's almost like killing two birds with one stone, we get a conditioning benefit out of it and they get their coaching benefits out of it. That's the way forward as we try to maximise our time. Everyone wants a piece of the pie, everyone wants a piece of the player's time and you just can't keep adding to it. You just can't keep training more and more and more, it's about trying to find a common ground.
For example every Wednesday we do conditioning games, they're fun, the players like them and the guys don't realise they're working that hard. But we're getting a conditioning benefit and Ian Costello is getting his decision making and continuity work so there's a two pronged attack.
We've just managed to get a deal with Catapult, a GPS company who have provided us with over twenty GPS units so we can quantify those conditioning games and we can see exactly what we're getting out of the players.
Tough times....
I think they say guys like us need a sadistic streak to be a strength & conditioning coach. We try and hide it but I get a grin on my face when we're running through a session and guys are struggling or guys are breaking, but I know I've done my job and I know it's a bit of an old school mentality but we need a bit of that.
Munster prides itself on work ethic and prides itself on doing more than everyone else and it's our job to monitor the players doing more than everyone else. But we need to be smarter about doing that because the more we do we don't want to risk them with injuries and what not.
But that is the culture here, and I could write the best physiological training programme that there is, but I really think there's a mental aspect to conditioning as well and that's 50% of what we do.
It's all about taking players to that dark place, so when they are in a game like Northampton last year and there's 41 phases in the last few minutes and they're off their feet and ROG kicks goal there's a perfect example of that feeling. I love seeing something like that because I feel as though in some way we have had an influence on them to go those 41 phases and not break mentally.
Players hate that yes and players thrive in it but you do learn a lot about players from it, and on occasion we sit there on the side-line with a smirk.
Players fronting up.....
I laid down the law before breaking up for off-season, and we set some sessions and tough work for the players to do on their own. We gave them 2 weeks off and I told them to build the guilt, and then they had 3 weeks of work to do on their own before they came back.
I threatened to send guys back to the academy training in Rockwell if they didn't come back in good shape and that was directed at all the squad. I wanted to and expected to send someone back.
But in the end I was trying to find someone to send just for the sake of dropping someone back because I was that impressed with them ,and now it looks a bit soft but I couldn't find anyone who hadn't done the work.
They've really come back in good nick and standouts for me would be Stephen Archer, he's come back in unbelievable shape. Sean Dougall who's come on board this year looks like from a physical aspect that he's an impressive athlete and even some of the young guys are fronting up. We had a very tough session last week where academy players Ronan O'Mahony and Corey Hircock stood out so it's across the board really.
Changes for next season.....
There have been big changes within the Strength & Conditioning dept. and within the whole department. Within mine I'm really happy where we're at.
I know we've only been at it three weeks but we've got Aidan O'Connell there, who's obviously been here a number of years, Will Cuddihy an intern from last year has come on board, Will Douglas who formerly worked with the Welsh Rugby Union side and PJ Wilson who's done some work with the Cork footballers and come through the academy system helping out there.
I couldn't be happier with the crew that we have, they're enthusiastic and have a great work ethic and I think the players are really responding to the personalities so that's fantastic.
Within the wider group we're really looking forward to the gaffer (Rob Penney) coming along. We've had skype calls with him, and the philosophies we have and what he wants from a strength & conditioning department seem very much aligned and we're looking forward to working with him and Simon Mannix.
We're all working really well together and I'm looking forward to the season ahead."


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text: Kotaku 'Shop Contest: What's He Really Playing? EditionOwen Good1/31/10 1:00pm2591EditPromoteGo to permalinkTwo weeks ago, MTV Multiplayer's Russ Frushtick invited Project Runway's Tim Gunn over to critique Mario's fashion sense - and blast on some virtual big game. And as a 'shop goes, this should be like shooting fish in a barrel.


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text:
The following Park Scholars and alumni received media coverage over the past couple of months:
Park Scholar Media Mentions
Park Scholar Media Mentions
The following Park community members have received media attention in recent weeks:
Evan Brooks '18 Makes Early Strides Toward a Career in Research and Teaching
Investigating possibilities, engaging in trial and error, cultivating relationships and resources: all are skills a researcher needs to be effective. With an eye to a career in academia, Evan Brooks '18 has been honing these skills since his high school days.
Park Scholar Media Mentions
Several Park Scholars have received media attention in recent months for their work on entrepreneurial initiatives and causes ranging from medicine to girls' empowerment, the environment, and affordable housing. Read on to learn how they're positively impacting their communities.
A Tale of Three Teachers
Across North Carolina's Triangle and Triad, Philip Boyne, Mollie Richardson Williams, and Jasmine Frantz are working to inspire the next generation of scholars and leaders. They are teaching more than physics, chemistry and math, respectively, as they bring the values learned as Park Scholars into the classroom.
Park Scholarships Alumni Find Fit with Deloitte's Corporate Culture
Although Park Scholars' backgrounds and interests are widely varied, many alumni have found a home at Deloitte. Deloitte provides audit, tax, consulting, enterprise risk, and financial advisory services for many of the largest companies in the world, including many of the Fortune 500.
Into the Woods: Retreats Foster Clarity and Connection
Retreats are integral components of the Park Scholarships experience. The Freshman Retreat serves to welcome new scholars into the community by affording them opportunities to get to know one another, the program itself, Park Faculty Scholars and staff, and the upperclassmen who facilitate small group discussion throughout the three-day period.
The Tyler Confrey-Maloney Study Abroad Grant: Investing in the Impact of International Experiences
In a survey of recent Park Scholarships graduates, nearly 90% of respondents reported having had at least one international experience over the course of their undergraduate years – whether study abroad, research, a professional conference, an internship, service, or an alternative pursuit.
Digging Deeper: Cave Dwellers Explore the Big Questions
As freshmen, Park Scholars participate in a weekly seminar that introduces topics related to leadership and service, and exposes students to the diversity of NC State's academic offerings and other resources. The seminar also provides a framework for how different disciplines approach problem solving.
Recent Park Graduates Give Back through AmeriCorps
Two recent Park alumni, Mary Charles Hale '13 and Alyson Harding '13, have spent the past several months participating in AmeriCorps, a federal government-run program which provides services in nonprofit organizations, schools, public agencies, and community and faith-based groups across the country.


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1 item from 2011
24 May 2011 11:21 AM, PDT | Destroy the Brain | See recent Destroy the Brain news »
This week's DVD and Blu-Ray Releases include a couple of films from the After Dark Horrorfest as well as two of Ron Howard's earlier films and a Blu-Ray edition of Solaris. Check beyond the break for the full list.
All Descriptions of the following titles are provided by Amazon.com unless otherwise noted. If you plan on buying a flick from this list, please click on the links provided or click on the cover as it helps us pay the bills around here. Also, unlike most sites, we provide the Netflix widget which we think is pretty convenient to add these films to your queue. If you don't have Netflix, feel free to click on "Free Trial" and try it out!
Death Hunter: Werewolves vs. Vampires
Format: DVD
In a forgotten region of the desert, an unspeakable evil exists. By no choice of his own, John Croix »
- Andy Triefenbach
1 item from 2011
IMDb.com, Inc. takes no responsibility for the content or accuracy of the above news articles, Tweets, or blog posts. This content is published for the entertainment of our users only. The news articles, Tweets, and blog posts do not represent IMDb's opinions nor can we guarantee that the reporting therein is completely factual. Please visit the source responsible for the item in question to report any concerns you may have regarding content or accuracy.See our NewsDesk partners


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Foremost Groups at ATGStores.com
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Foremost Groups
Recent Customer Reviews
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I''m a former Builder and Current Realtor.Read More
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For the price, perfect. well made and looks more expensive than it isRead More
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It''s just what I wanted! Solid wood except for the back. Love that it''s shallow, but still provides some much needed storage and it matched my existing vanity almost to a tee. Looks great! Great price!Read More
We live in a 1970''s home, and we''re updating our bathrooms. We could not find a medicine cabinet to fit in the existing hole so we opted to purchase a mirror. Well, we couldn''t find one to fit the existing space. For almost two years, I searched high and low both in store and online. I also ne…Read More
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If you are looking for a white framed mirror with a hint of "wainscoting" to pick up the rest of wainscoting in the room you are furnishing, you won''t find a better choice at this price range. I visited several home stores and scoured the internet and this is the nicest one out there. It is not solidRead More
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This is a very nice piece. The two drawers at the top hold all our makeup, etc. and the bottom door opens to two shelves that hold extra towels and paper. It comes fully assembled except for the shelf and the wood is beautiful. Matches my Allen & Roth Espresso vanity very well. I would recommend.Read More
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Kraaijenbrink, T., van Driem, G.L., Opgenort, J.R., Tuladhar, N.M. and de Knijff, P.
Notes: Allele frequency data for 21 autosomal loci was studied in 953 unrelated individuals belonging to 12 major Nepalese groups. DNA was extracted from whole blood using the Autopure LS® kit, then amplified using the PowerPlex® 16 System, AmpFlSTR® Identifiler® kit or F13A01, FESFPS, F13B, LPL Multiplex (FFFL) Multiplex. Amplification products were analyzed using the ABI PRISM® 3100 Genetic Analyzer. Several new alleles not reported in the NIST short tandem repeat database were detected in this population. (3811)
Expand Full Notes »
PowerPlex® 16 System
Brehm, R., Zeiler, M., Rüttinger, C., Herde, K., Kibschull, M., Winterhager, E., Willecke, K., Guillou, F., Lécureuil, C., Steger, K., Konrad, L., Biermann, K., Failing, K. and Bergmann, M.
Notes: To study the role of connexin42 (cx43) in testis development, the authors generated a conditional cx43 knockout mouse, which lacked the cx43 gene in Sertoli cells. To confirm that the cx43 gene was deleted in these mice, PCR was performed using primers specific to cx43, 1X Colorless GoTaq® Flexi Reaction Buffer, 2mM MgCl2, dNTPs and 0.15µl of GoTaq® DNA Polymerase. Tissue-specific deletion of cx43 was confirmed by amplifying RNA isolated from mouse testis, heart and tail was also confirmed using the same PCR components. (3713)
GoTaq® DNA Polymerase
Yu, Q., Collavo, A., Zheng, M.Q., Owen, M., Sattin, M. and Powles, S.B.
Notes: The authors characterized mutations in the acetyl-coenzyme A carboxylase (ACCase) gene that confer resistance to the herbicide clethodim in the grass weed Lolium rigidum. The ACCase gene was amplified from clethidem-resistant and susceptible plants, then sequenced to identify previously unknown mutations. Amplifications of ACCase were performed using 300ng of genomic DNA and GoTaq® Green Master Mix. The Wizard® SV Gel and PCR Clean-Up System was used to purify PCR products directly or from agarose gels. (3721)
Wizard® SV Gel and PCR Clean-Up System
GoTaq® Green Master Mix
Stavridis, M.P., Lunn, J.S., Collins, B.J. and Storey, K.G.
Notes: The authors studied the role of the Erk1/2 signaling pathway during neural specification in mouse embryonic stem (ES) cells. Undifferentiated ES cells express high levels of the pluripotent marker Nanog but do not express fibroblast growth factor (Fgf5), an early marker of differentiation of ES cells, or Sox1, an early neural transcription factor gene. Using quantitative PCR, levels of Nanog, Fgf5 and Sox1 mRNA were quantitated during ES differentiation in the presence and absence of a MEK inhibitor. Prior to quantitative PCR, 1µg of total RNA was reverse transcribed using ImProm-II™ Reverse Transcriptase. By measuring these mRNA levels, the authors determined that inhibition of the Erk1/2 pathway blocked ES differentiation. (3726)
ImProm-II™ Reverse Transcriptase
Kanayama, T., Arito, M., So, K., Hachimura, S., Inoue, J. and Sato, R.
Notes: To explore the interaction of liver receptor homolog (LRH)-1, a known suppressor of sterol regulatory element-binding protein (SREBP) transcriptional activity, human LRH-1 was reverse transcribed then amplified by PCR from total RNA from HepG2 cells. The amplification product was ligated into the pTargeT™ Mammalian Expression Vector to create pTarget-LRH1. For reporter experiments, a PCR fragment that encompassed the 1.3kb 5'-promoter region of the human small heterodimer partner (SHP) gene was cloned into the pGL3-Basic Vector (designated pSRB). The pGL3-Promoter Vector was used to construct pLRHREx3, which contains three LRH-1 response elements, and the insert was generated using synthetic oligonucleotides. HEK293 cells were cotransfected with 0.2µg of a promoter-firefly luciferase construct, 0.1µg of a SREBP expression plasmid, 10ng of phRL-TK Vector and 0.2 or 0.6µg of pTarget-LRH1. Alternatively, the cotransfected plasmids were 0.2µg of pSHP, 0.1µg of pTarget-LRH1, 10ng of phRL-TK Vector and 0.2 or 0.6µg of a SREBP expression plasmid. The pLRHREx3 construct (0.2µg) was cotransfected with 0.1µg of a LRH-1 expression plasmid, 0.2µg of pCMXPGC-1α (peroxisome proliferator activated receptor γ coactivator-1α), 10ng of phRL-TK Vector, and 0.1 or 0.3µg of a pSREBP expression vector in HEK 293 cells. Luciferase expression was assayed 48 hours post-transfection using the Dual-Luciferase® Assay Reporter System. To express SREBPs and LRH-1 in vitro, inserts were ligated into the pTNT™ Vector, synthesized using the TNT® Coupled Transcription/Translation System with radiolabeled methionine. Ten microliters of the 35S-labelled protein was then used in a GST-pulldown assay. (3692)
Dual-Luciferase® Reporter 1000 Assay System
Dual-Luciferase® Reporter Assay System
Dual-Luciferase® Reporter Assay System 10-Pack
pGL3-Basic Vector
pGL3-Promoter Vector
pTnT™ Vector
TnT® SP6 Coupled Reticulocyte Lysate System
TnT® SP6 Coupled Reticulocyte Lysate System, Trial Size
TnT® T3 Coupled Reticulocyte Lysate System
TnT® T7 Coupled Reticulocyte Lysate System
TnT® T7 Coupled Reticulocyte Lysate System, Trial Size
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Adams, B.D., Furneaux, H. and White, B.
Notes: This study investigated the mechanism of silencing of the estrogen receptor α mRNA in the human breast cancer cell line, MCF-7. The authors initially used software for miRNA target prediction to analyze the 3´ UTR of the human ERα gene for potential miR-206 target sites. Two potential targets, designated hERα1 and hERα2, were identified. ERα levels were repressed in a dose-dependent manner in MCF-7 cells transfected with a synthetic pre-miR-206 duplex, and transfection of an miR-206 expression construct into MCF-7 cells also resulted in specific inhibition of ERα expression, as measured by real-time PCR and Northern blot assays. A luciferase reporter assay was then used to determine whether miR-206 interacted directly with the hERα1 and hERα2 sites in the ERα 3´UTR. Luciferase reporter constructs containing either the hERα1 or hERα2 cloned 3´ of the firefly luciferase gene showed miR-206-medisted repression of luciferase expression in HeLa cells. Mutation of the hERα1 or hERα2 sites to disrupt hybridization with the 5´ region of miR-206 restored luciferase activity, as did co-transfection with an miRNA antagonist of miR-206. Transformation of the luciferase constructs into the breast cancer cell lines, MCF-7 and MDA-MB-231, both of which expressed high levels of miR-206 as measured by real-time PCR, resulted in repression of luciferase activity. Treatment with estrogen was then shown to reduce miR-206 levels in MCF-7 cells. Luciferase assays were used to confirm this result, and levels of luciferase activity from the reporter constructs were increased upon exposure to estrogen, indicating that ERα agonists were able to decrease miR-206 levels in MCF- cells. (3603)
Luciferase Assay System
Cho, Y.Y., Yao, K., Bode, A.M., Bergen, H.R. 3rd, Madden, B.J., Oh, S.M., Ermakova, S., Kang, B.S., Choi, H.S., Shim, J.H. and Dong, Z.
Notes: The CheckMate™ Mammalian Two-Hybrid System was used to screen for protein-binding partners for RSK2, a ribosomal S6 kinase involved in myoblast differentiation. The RSK2 cDNA was cloned into the pBIND Vector as bait while several transcription factors were amplified by PCR and cloned into the pACT Vector. The bait pBIND-RSK2 construct, the pACT-transcription factors and pG5-luc Vector were transfected into 293 cells at a 1:1:1 molar ratio. To assess the protein interaction, the cells were lysed, and the firefly luciferase activity was normalized to Renilla luciferase activity. The strongest interaction was with NFAT3. (3574)
CheckMate™ Mammalian Two-Hybrid System
Soltani-Bejnood, M., Thomas, S.E., Villeneuve, L., Schwarz, K.T., Hong, C.S. and McKee, B.D.
Notes: In this paper, the authors studied the common region of mod(mdg4) and its role in homolog conjunction during meiosis. Genomic DNA was isolated from adult Drosophila using the Wizard® Genomic DNA Purification Kit and mutations identified by PCR and sequencing of the introns. (3576)
Wizard® Genomic DNA Purification Kit
Iguchi, K. et al.
Notes: In this paper, the authors hypothesized that bisphosphonates, which are used to prevent tumor metastasis, affect expression of urokinasetype plasminogen activator (uPA), which seems to be critical for prostate cancer metastasis. The authors examined the effect of several bisphosphonates on uPA expression in PC-3 cells. Pamidronate treatment resulted in lower uPA mRNA levels. To investigate the cause, the authors created a uPA reporter construct (pGL3-uPA) by cloning the 5′-flanking region of the human uPA gene upstream of a firefly luciferase reporter gene in the pGL3-Basic Vector. PC-3 cells were seeded at a density of 3 × 104 cells/well in 24-well culture plates and transfected with 0.5µg of pGL3-uPA and 1ng of the Renilla luciferase phRL-TK Vector using FuGENE® 6 Transfection Reagent. At 48 hours post-transfection, the authors measured reporter activity using the Dual-Luciferase® Reporter Assay System to learn that treatment with 100µM pamidronate inhibited transcription of the uPA gene. (4384)
Barrachina, M., Moreno, J., Juvés, S., Moreno, D., Olivé, M. and Ferrer, I.
Notes: These authors used chromatin immunoprecipitation to show that neuron-restrictive silencer factor interacts with the ubiquitin carboxy-terminal hydrolase L1 (UCHL1) promoter in U87-MG, DMS53 and HeLa cells. The neuron-restrictive silencing element (NRSE1) of the UCHL1 promoter was amplified using GoTaq® Flexi DNA Polymerase in a 25µl PCR. (3705)
GoTaq® Flexi DNA Polymerase
Gierman, H.J., Indemans, M.H., Koster, J., Goetze, S., Seppen, J., Geerts, D., van Driel, R. and Versteeg, R.
Notes: The authors explored the possibility of a domain-based level of gene expression regulation for chromosomes by integrating the green fluorescent protein (GFP) reporter in 90 different locations in cultured human cells. This integration was accomplished by infecting human embryonic kidney cells (HEK293) with a lentiviral construct carrying the GFP gene under the control of the ubiquitously expressed human phosphoglycerate kinase (PGK), sorting GFP-positive cells by FACS and selecting clones for expansion. Genomic DNA was isolated from the various clones using the Wizard® SV Genomic DNA Purification System and analyzed by PCR or restriction digested for Southern blotting. (3743)
Wizard® SV Genomic DNA Purification System
Pilecka, I., Patrignani, C., Pescini, R., Curchod, M.L., Perrin, D., Xue, Y., Yasenchak, J., Clark, A., Magnone, M.C., Zaratin, P., Valenzuela, D., Rommel, C. and van Huijsduijnen, R.H.
Notes: To genotype PTPH1 knock-out (KO), heterozygous (HET), and wild type (WT) mice, tail snips were digested overnight with proteinase K and the DNA trapped using the Wizard® SV 96 Genomic DNA Purification System. Genomic DNA was washed using the Wizard® SV Wash Solution and eluted in 200μl of water at 65°C. The protease was inactivated at 95°C and 2μl of DNA was used for PCR. (3739)
Wizard® SV 96 Genomic DNA Purification System
Gluckman PD, Lillycrop KA, Vickers MH, Pleasants AB, Phillips ES, Beedle AS, Burdge GC, Hanson MA.
Notes: To examine how exposure to leptin in utero affects hepatic gene expression and epigenetic status in adulthood, pregnant Wistar rats were fed a standard diet or 30% undernutrition. The three-day-old pups were exposed to saline or leptin for 10 days, weaned and either fed a standard or high-fat diet. On day 170, the rats were sacrificed and tissues snap frozen. Five micrograms of genomic DNA was isolated from rat liver using the Wizard® SV Genomic DNA Purification System. Then 25ng of the purified DNA was digested with methylation-sensitive restriction enzymes, and the glucocorticoid receptor (GR) and peroxisome proliferator-activated receptor alpha (PPARα) fragments were amplified by real-time PCR. (3744)
Gershon, E., Hourvitz, A., Reikhav, S., Maman, E. and Dekel, N.
Notes: The authors investigated the role of estrogen inactivation by the SULT1E1-encoded estrogen sulfotransferase in ovulation in mice. Semiquantitative RT-PCR was used to characterize the temporal and tissue-specific expression of SULT1E1 mRNA in ovulating mice. First-strand cDNA synthesis was performed at 37°C for 2 hours using 7.5µg of total RNA, 1µl (0.5µg) of oligo(dT)15, 40 units of RNasin® Ribonuclease Inhibitor and 200 units of M-MLV Reverse Transcriptase. (3913)
M-MLV Reverse Transcriptase
Oligo(dT)15 Primer
RNasin® Ribonuclease Inhibitor
Clauser, S., Peyrard, S., Gaussem, P., Crespin, M., Emmerich, J., Aiach, M. and Borgel, D.
Notes: To examine the effects of hormones on Gas6, a plasma vitamin K-dependent protein that may play a role in cardiovascular disease, the authors developed an ELISA test for Gas6, which they tested on blood from male and female volunteers. A recombinant Gas6 control was developed by reverse transcribing the full-length human Gas6 mRNA from human umbilical vein endothelial cells, amplifying the cDNA using nested PCR and after restriction digestion, ligating the insert into the EcoRI and XbaI sites of the pCI-neo Mammalian Expression Vector. The full-length construct was confirmed by sequencing and then tested in the Gas6 ELISA. (3688)
pCI-neo Mammalian Expression Vector
Ichimura, S., Nagano, M., Ito, N., Shimojima, M., Egashira, T., Miyamoto, C., Ohkusu, K., and Ezaki, T.
Notes: These authors compared standard culture conditions, DNA isolation and analysis (e.g, sequencing) with a liquid culture, DNA isolation and a homogeneous fluorescent detection system for identifying mycobacterial species. The standard DNA extraction began with a loopful (3–mm3 sphere) of bacterial colony grown on Ogawa slants that used glass beads to mechanically disrupt the cells. The resulting lysate was extracted using phenol/chloroform, and DNA purified from the aqueous phase using a robotic liquid handler AGE-96 (Biotec) and the MagneSil® Blood Genomic, Max Yield System. The DNA extractions were used in PCR and sequencing reactions. (3700)
MagneSil® Blood Genomic, Max Yield System
Iguchi, H., Urashima, Y., Inagaki, Y., Ikeda, Y., Okamura, M., Tanaka, T., Uchida, A., Yamamoto, T.T., Kodama, T. and Sakai, J.
Notes: Sex-determining Y-box (SOX) 6 is a transcription factor downregulated in obesity-related insulin-resistant animals. The authors examined the interaction between SOX 6 and β-catenin, a protein that modulates cyclin D1 promoter activity. To characterize the physical interaction, in vitro binding assays were performed using GST-fused SOX 6 and deletion mutants of β-catenin, which were expressed as 35S-labeled proteins in the TNT® T7 Quick Coupled Transcription/Translation System. The GST-fusion proteins were bound to MagneGST® particles and allowed to interact with the β-catenin mutants. Purified GST was used as a negative control to determine nonspecific protein binding. The authors were able to identify the protein domains necessary for SOX 6/β-catenin interaction. Similar binding assays were performed with GST-β-catenin and 35S-labeled T-cell factor in the presence or absence of SOX 6 to show that SOX 6 does not interfere with the binding of β-catenin to TCF. (3685)
MagneGST™ Pull-Down System
TnT® T7 Quick Coupled Transcription/Translation System
TnT® T7 Quick Coupled Transcription/Translation System, Trial Size
Treeck, O., Pfeiler ,G., Horn, F., Federhofer, B., Houlihan, H., Vollmer, A., and Ortmann, O.
Notes: This study identified two novel transcript variants of the estrogen receptor ERβ that were expressed in the ERα-negative breast cancer cell line MDA-MD-231. These variants were identified after amplification of ERβ transcripts from the breast cancer cell line by RT-PCR. The amplification products were then excised from gels and subcloned into the pTARGET™ Mammalian Expression Vector prior to sequencing. COS1 cells, which do not express the estrogen receptor, were then stably transfected with full-length ERβ or one of the splice variants and the effects on cell proliferation, apoptosis, and estrogen response were evaluated. In COS1 cells expressing either ERβ or the transcript variants cell proliferation decreased and basal apoptosis (caspase 3/7 activity) increased, compared to cells transfected with vector alone. Exposure to therapeutic doses of tamoxifen induced apoptosis in cells expressing the full-length ERβ but not in cells expressing either of the variant isoforms. (3618)
CellTiter-Blue® Cell Viability Assay
M-MLV Reverse Transcriptase Buffer Pack
M-MLV Reverse Transcriptase, RNase H Minus
pTargeT™ Mammalian Expression Vector System
Caspase-Glo® 3/7 Assay
Narkuti, V., Vellanki, R.N., Gandhi, K.P., Doddapaneni, K.K., Yelavarthi, P.D. and Mangamoori, L.N.
Notes: The authors describe two cases of paternity dispute, one with a maternally mismatched allele at the D18S51 locus and a second with a paternally mismatched D18S51 allele. Seventeen autosomal STR loci were analyzed using the PowerPlex® 16 System and AmpFlSTR® Identifiler® kit. Amplifications were performed using a GeneAmp® PCR System 9700, and amplification products were detected using an ABI PRISM® 310 Genetic Analyzer. Y-STR loci and mitochondrial DNA hypervariable regions HV1 and HV2 were also examined. Sequence analysis of the D18S51 locus revealed an expansion of the maternal allele by one repeat unit in one case and an expansion of the paternal allele by two repeat units in the second case. (3809)
Hill, C.R., Kline, M.C., Mulero, J.J., Lagacé, R.E., Chang, C.W., Hennessy, L.K. and Butler, J.M.
Notes: The authors analyzed 1,308 samples for concordance between the Identifiler® kit, AmpFlSTR® Minifiler™ kit and PowerPlex® 16 System. DNA was isolated from liquid blood using the manual DNA IQ™ System protocol, and STR amplifications were performed as per the manufacturer's recommendations except that reaction volumes were decreased by half. Amplified products were analyzed using an Applied Biosystems 3130xl and POP™-4 or POP™-6 polymer. Twenty seven disconcordant phenotypes were identified between the Minifiler™ and Identifiler® kits, 14 between Minifiler™ and PowerPlex® 16 kits, and 4 between PowerPlex® 16 and Identifiler® kits. (3770)
DNA IQ™ System
Kommagani, R., Payal, V. and Kadakia, M.P.
Notes: The authors examined transcriptional regulation of the vitamin D receptor (VDR) by p53 and p63, a member of the p53 family, under stressed and unstressed conditions. Reporter constructs with the full-length and minimal VDR promoters controlling expression of firefly luciferase were cotransfected with p53 or p63 expression constructs, and transcriptional activation of the VDR promoter was monitored using the Dual-Luciferase® Reporter 100 Assay System. Results were normalized to Renilla luciferase activity. Interaction between p73, another member of the p53 family, and the VDR promoter was examined using chromatin immunoprecipitation. The imunnopreciptated chromatin was reverse crosslinked, DNA was eluted and VDR and p21 sequences were detected by PCR using GoTaq® Green Master Mix. (3715)
Borawski, J., Lindeman, A., Buxton, F., Labow, M. and Gaither, L.A.
Notes: A lentiviral expression vector containing the firefly luciferase gene from a pGL3 Vector was transduced into SKOV3 cells in 384-well plates, transfected with various siRNAs and analyzed 72 hours later. The luciferase expression was determined using the Bright-Glo™ Luciferase Assay System and cell viability assessed using the CellTiter-Glo® Luminescent Cell Viability Assay. (3729)
CellTiter-Glo® Luminescent Cell Viability Assay
Bright-Glo™ Luciferase Assay System
Fan, F. and Wood, K.V.
Notes: The authors of this paper review bioluminescent assay technologies, discussing HTS reporter, cell-based and luciferase biosensor assays. They divide luminescent assays into three basic categories: assays that measure ATP concentration (cell viability and kinase assays), assays that measure changes in luciferase levels (reporter assays, GPCR assays), and assays that measure changes in luciferin levels (protease [including caspase], P450 and MAO assays). (3737)
BacTiter-Glo™ Microbial Cell Viability Assay
Calpain-Glo™ Protease Assay
cAMP-Glo™ Assay
Caspase-Glo® 2 Assay
Caspase-Glo® 6 Assay
Caspase-Glo® 8 Assay
Caspase-Glo® 9 Assay
Dual-Glo® Luciferase Assay System
Kinase-Glo® Luminescent Kinase Assay
Kinase-Glo® Plus Luminescent Kinase Assay
MAO-Glo™ Assay
P450-Glo™ CYP1A1 Assay
P450-Glo™ CYP1A2 Assay
P450-Glo™ CYP1A2 Screening System
P450-Glo™ CYP1B1 Assay
P450-Glo™ CYP2C19 Assay
P450-Glo™ CYP2C19 Screening System
P450-Glo™ CYP2C8 Assay
P450-Glo™ CYP2C9 Assay
P450-Glo™ CYP2C9 Screening System
P450-Glo™ CYP2D6 Assay
P450-Glo™ CYP2D6 Screening System
P450-Glo™ CYP3A4 Assay
P450-Glo™ CYP3A4 Assay (Luciferin-PFBE)Cell-Based/Biochemical Assay
P450-Glo™ CYP3A4 Assay (Luciferin-PPXE)DMSO-Tolerant Assay
P450-Glo™ CYP3A4 Screening System
P450-Glo™ CYP3A4 Screening System (Luciferin-PPXE)DMSO-Tolerant Assay
P450-Glo™ CYP3A7 Assay
Renilla Luciferase Assay System
Su, H.W., Yeh, H.H., Wang, S.W., Shen, M.R., Chen, T.L., Kiela, P.R., Ghishan, F.K. and Tang, M.J
Notes: The authors tested their hypothesis that Na+-H+ exchangers (NHE) are involved in the formation of multicullar dome structures in confluent Madin-Darby canine kidney (MDCK) cells and that the Stat3 pathway is involved in regulation of NHEs. The authors performed semi-quantitative RT-PCR to monitor NHE3 mRNA levels in MDCK cells expressing a constitutive Stat3 mutant or a dominant-negative Stat3 mutant. The reverse transcription step was performed using Promega M-MLV Reverse Transcriptase. RAlso, Stat3 activities in low-density cultures and high-density cultures were compared using a reporter gene assay. Four copies of the Stat3-binding site were cloned upstream of a firefly luciferase reporter gene, and the resulting vector, along with the pRL-TK Vector for normalization, were transfected into MDCK cells. Luciferase activities were measured using the Dual-Luciferase Reporter Assay System. (3910)
pRL-TK Vector
Renaud, S., Loukinov, D., Abdullaev, Z., Guilleret, I., Bosman, F.T., Lobanenkov, V. and Benhattar, J.
Notes: Telomeres shorten by 50–100 bases with each cell division, making the telomere a "mitotic counter" that can limit cellular lifespan. Telomerase is a two-component protein consisting of a reverse transcriptase (hTERT) bound to its own RNA template that can act to maintain telomere length in dividing cells. Telomerase is highly active in dividing cells such as germ cells, stem cells and many cancers. This paper investigated the role of methylation of the hTERT promoter and the transcription factor CTCF in regulation of telomerase activity. LacZ reporter plasmids driven by the hTERT minimal promoter were transiently transfected into HeLa cells, and reporter assays were performed on lysate generated using Passive Lysis Buffer. The hTERT minimal promoter did not show activity if all of the CpG sites were methylated. The promoter and first exon of hTERT were amplified using PCR Master Mix from sodium bisulfite-treated genomic DNA isolated from telomerase-positive cell lines and tissues. The resulting fragments were cloned using the pGEM®-T Vector System II. For the methylation cassette assay, methylated and unmethylated fragments were cloned into a methylated or unmethylated vector using the LigaFast™ Rapid DNA Ligation System. The authors conclude that methylation plays a dual role in regulating hTERT expression. CTCF will bind to the first exon of hTERT when the hTERT CpG island is not methylated, resulting in downregulation of hTERT expression. Although CTCF cannot bind the hTERT promoter when the DNA is completely methylated, the methylation itself completely represses transcription. In situations where there is partial methylation of the promoter, such as in tumor cells, CTCF cannot bind to the promoter, but the partial methylation is not enough to repress transcription, and hTERT is expressed. (3641)
LigaFast™ Rapid DNA Ligation System
PCR Master Mix
pGEM®-T Vector System II
Passive Lysis 5X Buffer


text:
Category:Dudley Farm Historic State Park
From Wikimedia Commons, the free media repository
This is a category about a place or building that is listed on the National Register of Historic Places in the United States of America. Its reference number is 02001081
English: Dudley Farm Historic State Park, a Florida State Park and on the U.S. National Register of Historic Places.
This category has the following 4 subcategories, out of 4 total.
- ► Dudley Farm animals (4 F)
- ► Dudley Farm Visitor Center (2 F)
- ► Newberry Dudley Farm buildings (12 F)
- ► Newberry Dudley Farm house (6 F)
Media in category "Dudley Farm Historic State Park"
The following 4 files are in this category, out of 4 total.
- Newberry Dudley Farm entr01.jpg 2.05 MB
- Newberry Dudley Farm entr02.jpg 1.08 MB
- Newberry Dudley Farm entr03.jpg 1.42 MB
- Newberry Dudley Farm fence01.jpg 2.28 MB


text:
What the fuck have things come to as of late? I mean shit, I think us Bang Bros. need to look into some AA meetings... because this shit is getting out of hand! Check the scenario... After a night of wild drinking and partying.. this guy named Cliff that I supposedly know from some point in my past was next to me driving the Bang Bus.. how strange was that? I then proceeded to give Ugs a call to see where the fuck he was and he tells me he fell asleep somewhere on the beach and for us to go pick him up.... well my fine feathered friends.. this is where the plot thickens.... upon arrival, I see that Ugly is hanging out with a real live dwarf... a fucking DWARF! I know.. bugged me out too... but god-damn it all to fiery hell if this chick wasn't looking right! Lil' Diva's huge round ass had me turned on... which isn't creepy.. is it? Anyways.. Lil Diva made a fine addition to our crew ... so we were off! After turning and twisting thru downtown Miami.. we came across the sexy Alice... this german honey dip was backpacking thru the U.S. and was searching for a way to get to South Beach.. muahahahaha.... my specialty! I thought it was just perfect for us to roll with Alice... being that she's from Munich, Germany... "where the beer is from" as Alive put it.... so we were off! Diva did a fine job of getting Alice to get comfortable in the cauldron of debauchery.... I love this chick! So yeah, after briefly discussing how progressive these euros are.. it was time to get down to it... Having Diva and Alice strip down before our eyes was fucking great! Nothing better than two pairs of great natural boobs and round butt cheeks jiggling the day away... but the real deal didn't have shit on these antics.. Alice's blowjobs were great to film.... nice and messy is how I like 'em... Then my boy Cliff went ahead and fucked the shieza out of Alice... dick to pussy action is always good.. no? YADA YADA YADA... we dropped her off in the outskirts of South Beach.. you know.. a good 20 miles away.. haha! What... you think because the broad is foreign she'd get pity.... have you forgotten about my euro adventures?!?!?! HAHAHA! The Dirty One...
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text:
Humor: Weird Al — "White & Nerdy"
White & Nerdy (2006), is a fantastically funny rap song, sung by "Weird Al" Yankovic.
Here's the lyrics:
They see me mowin' My front lawn I know they're all thinking I'm so White n nerdy
Think I'm just too white n nerdy Think I'm just too white n nerdy Can't you see I'm white n nerdy Look at me I'm white n nerdy!
I wanna roll with The gangsters But so far they all think I'm too white n nerdy
Think I'm just too white n nerdy Think I'm just too white n nerdy I'm just too white n nerdy Really, really white n nerdy
MC Escher artist drawing math related art. See Art of M C Escher • Projective Illusion • Algorithmic Mathematical Art (p3).
Earl Grey tea favorite drink of Captain Picard of Star Trek.
First in my class here at M.I.T. Got skills, I'm a Champion of DND MC Escher that's my favorite MC Keep your 40 I'll just have an Earl Grey tea
Steven Hawkings Stephen Hawking's most popular book is A Brief History of Time. Similar book of that era is Godel, Escher, Bach by Douglas Hofstadter and The Emperor's New Mind by Roger Penrose.
My rims never spin to the contrary
You'll find they're quite stationary
All of my action figures are cherry
Steven Hawkings in my library
MySpace One of the early most popular social networking site. Others of that erar includes LiveJournal, Blogger.
My MySpace page is all totally pimped out
I got people begging for my top 8 spaces
Yo I know Pi to a thousand places
Ain't got no grills but I still wear braces
minesweeper A popular puzzle game included in Microsoft Windows 95. See also Great Math Programs on Puzzles.
I order all of my sandwiches with mayonnaise
I'm a whiz at minesweeper I can play for days
Once you see my sweet moves you're gonna stay amazed
my fingers movin' so fast I'll set the place ablaze
killer app Means a very popular and cool software. Example includes: Mathematica, Emacs, Google Earth, and web browser.
There's no killer app I haven't run
At Pascal, well, I'm number 1
Do vector calculus just for fun
I ain't got a gat but I gotta soldering gun (what?)
Klingon fiction universe of Star Trek, Klingon is a language of the race Klingon. Klingon has been developed into a full constructed language. Another constructed language is lojban.
Happy days is my favourite theme song I can sure kick your butt in a game of ping pong I'll ace any trivia quiz you bring on I'm fluent in JavaScript as well as Klingon or I sing on
They see me roll on, my Segway! I know in my heart they think I'm white n nerdy!
Think I'm just too white n nerdy Think I'm just too white n nerdy Can't you see I'm white n nerdy Look at me I'm white n nerdy
I'd like to roll with- The gangsters Although it's apparent I'm too White n nerdy Think I'm just too white n nerdy I'm just too white n nerdy How'd I get so white n nerdy?
X-men from superhero comics. Superhero Landing
ergonomic keyboard Ergonomic Keyboard Reviews
I've been browsing, inspectin' X-men comics you know I collect 'em The pens in my pocket I must protect 'em my ergonomic keyboard never leaves me bored
Wikipedia I LOVE Wikipedia. I've been using and editing it since 2004. My website has near 4 thousand links to it, collected here: Links To Wikipedia from XahLee.org. Wikipedia itself has about 50 links to my website.
Shopping online for deals on some writable media
I edit Wikipedia
I memorized Holy Grail really well
I can recite it right now and have you ROTFLOL
HTML A tech for creating web pages. See Xah HTML Tutorial
I got a business doing websites
When my friends need some code who do they call?
I do HTML for them all
Even made a homepage for my dog!
Yo! Got myself a fanny pack they were having a sale down at the GAP Spend my nights with a roll of bubble wrap POP POP! Hope no one sees me gettin' freaky!
chess I played chess for few years in the early 1990s. But mostly, am more interested in the theoretical aspects of these types of games. See Go Variations on Tilings, Go as Cellular Automata.
I'm nerdy in the extreme and whiter than sour creme
I was in AV club and Glee club and even the chess team!
Only question I ever thought was hard
Was do I like Kirk or do I like Picard?
I spend every weekend
at the renaissance fair
I got my name on my under wear!
They see me strollin' They laughin' And rollin' their eyes 'cause I'm so white n nerdy
Just because I'm white n nerdy Just because I'm white n nerdy All because I'm white n nerdy Holy cow I'm white n nerdy
I wanna bowl with the gangsters but oh well it's obvious I'm white n nerdy
Think I'm just too white n nerdy Think I'm just too white n nerdy I'm just too white n nerdy Look at me I'm white n nerdy!
Buy best headphone, Sennheiser HD 598


text:
- Author: Christopher Hitchens
- Format:Paperback
- Publisher:Atlantic Books
- Published:04 December 2010
Sorry, there are currently no sellers for this product.
Product description
- Total Pages: 435
- Genre: Memoirs
- Secondary Genre: Press & journalism
- Illustrations: Illustrations, ports.
In this long-awaited and candid memoir, Hitchens re-traces the footsteps of his life to date, from his childhood in Portsmouth, with his adoring, tragic mother and reserved Naval officer father; to his life in Washington DC, the base from which from he would launch fierce attacks on tyranny of all kinds. Along the way, he recalls the girls, boys and booze; the friendships and the feuds; the grand struggles and lost causes; and the mistakes and misgivings that have characterised his life. Hitch-22 is, by turns, moving and funny, charming and infuriating, enraging and inspiring. It is an indispensable companion to the life and thought of our pre-eminent political writer.
Author's Biography
Christopher Hitchens (1949-2011) was a contributing editor to Vanity Fair and Visiting Professor in liberal studies at the New School in New York. He was the author of numerous books, including works on Thomas Jefferson, Thomas Paine, George Orwell, Mother Theresa, Henry Kissinger and Bill and Hillary Clinton, as well as the international bestseller and National Book Award nominee, god Is Not Great.


text:
Convert Joomla to Wordpress
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Project Description
I have a blog site in Joomla 1.5, I'm thinking to get it converted into the latest version of Wordpress or blogger but I'm open to suggestions. I just want easier to manage blog platform and I can add pages if I need to. Please suggest a template.
It is now hosted in VPS, I want the wordpress conversion is by hosted free by Wordpress or by a uk shared host. If you think it's better hosted by a paid shared host, please also suggest a cheap but reliable UK host because my domain is co.uk. You'll have to point my domain to new conversion after the job is completed. You'll have to make sure all links are working and the site is secure.
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- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online


text:
With fewer than 2,500 giant pandas left in the wild and 312 living in captivity, every breeding pair is vital to the continued survival of the species. Little wonder that the imminent arrival in Britain of a couple with a successful reproductive history – the first pandas to be in the UK for 17 years – has been eagerly anticipated by conservationists and animal lovers.
But only months before Tian Tian and Yang Guang move into their new home, the crisis engulfing their soon-to-be hosts at Edinburgh Zoo deepened yesterday as the chairman of the historic institution was forced to quit. Announcing his decision to stand down, Donald Emslie said recent events had been "incredibly difficult" after months of controversy in which the zoo has been beset by smears and infighting.
Last week he was overwhelmingly defeated in a no-confidence vote by the Royal Zoological Society of Scotland (RZSS). "As a board we have been dealing with a malicious and untrue attacks on both the society and a member of the senior management team," Mr Emslie said.
Last week an inquiry cleared the zoo's acting head, Gary Wilson, who had been the victim of what was described as a "deeply unpleasant and malicious smear campaign". He was suspended after several anonymous allegations.
- More about:
- London Zoo
- Management
- Zoology
- Zoos


text:
Grand Hotel Casino
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Looooong night
Last night we set off from Puerto Iguacu in Argentina to get to Gualeguaychu (wah-le-watch-oo) still in Argentina but close to the Uruguayian border. We got on at half 5 so played some games until bedtime. Managed to get some sleep. Once we got to wallawallabingbong we got another bus to cross the border. Had to actually get off the bus and ...
Who Made All The Pies???
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Klappriges Taxi, heisses Wasser & Sylvesterabend
... br>
Als wir an der Bushaltestelle standen, wussten alle Personen wann der Bus f�hrt obwohl es kein Fahrplan gab. Sonst heisst es einfach mal warten bis der richtige Bus kommt. Wenn man Pech hat, kann dies gut und gerne mehrere Stunden dauern. Dies macht nichts, weil hier in S�damerika die Leute immer Zeit haben und sich nicht stressen lassen.Der Bus war schon voller Menschen als wir eingestiegen waren. Doch unterwegs wurden immer noch Passagiere aufgenommen. Bei so ...
A lovely breakfast
... It was hot and humid though and made for sweaty cycling, with little shade offered along the way.
We stopped a couple of times for drinks and to get a sandwich at a petrol station, but other than that we just tried to cover the mileage for the day and the scenery gave us very little inspiration to stop for photos. It seems like the local people know that the scenery here isn't as interesting as in other parts of South America, but instead give us lovely encounters, ...


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FAA Gives Disney Permission To Fly Drones At Theme ParksGovernment aviation authorities have given Disney permission to fly drones at the company's theme parks in Florida and California.
Soaring Complaints Over Loud Jets At BWI AirportLoud jets taking off and landing at BWI Marshall are fueling frustrations from people living near the airport.
New Drone Rules Expected to Generate Billions for U.S.The long-awaited new rules allowing commercial use of drones is now in effect and could generate billions for the U.S. economy.
FAA Rule Change Restores Student Pilot Birthday SolosThe AOPA says a federal rule change has restored the ability of student pilots to fly solo on their 14th or 16th birthday.
Neighbors Fed Up With Noise From BWI AirportThe number of noise complaints at BWI Thurgood Marshall has doubled in a one-year span since the airport upgraded its technology and shifted its flight patterns.
FAA Unveils New Drone Rules To Make Flying EasierThe FAA unveils new landmark rules that make it easier to pilot an unmanned aircraft. George Solis reports.
BWI Control Tower Evacuated Briefly After Fire AlarmThe FAA tower at BWI Thurgood Marshall airport was evacuated this morning for a fire alarm.
FAA: Software Update May Be Responsible For Malfunction The Federal Aviation Administration says a technical problem at an air traffic control center in Virginia that caused hundreds of flights to be delayed or canceled was possibly caused by a software upgrade at a high-altitude radar facility.
Flights Back To Normal After FAA Automation NightmareA day after flights were canceled or delayed at BWI due to a computer glitch, things are back to normal.
Amazon One Step Closer To Launching Drone Delivery ServiceAmazon is one step closer to launching its drone delivery service. The Federal Aviation Administration recently approved the retail company to fly its unmanned aircraft on a trial basis.
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FAA Could Allow Commercial Drone Use In USNew technology may soon be taking to the skies. The FAA has released proposed requirements that could allow commercial drone use in the US.