text:
If you're going to have some renovation work done maybe a simple spring cleaning at home, proper waste management ought to taken into point. Home projects like these can accumulate a lot of waste, so in order to prevent having mess lying all over your place, you should want to hire a skip to dispose your clutter. Skips are just large heavy duty bins that can store large quantities of waste. It is an efficient and practical way of storing and disposing waste because once you're done filling it up, the skip hire company are the one in domination over collecting and disposing your trash.

Prohibited epidermis waste - You can put most things in skip, except: electrical items, batteries, tyres, paint tins, hazardous materials and liquid stuff. Your skip bin hire provider must inform you about this beforehand.

You additionally be use the mini skip hire adelaide to dispose off all your waste. Might be a convenient option for want get rid of your garbage regularly. Treatment needs to become carried out after all the garbage has been accumulated. Always be preferable if you keep hefty and dry garbage as a stand alone. You need to adopt care possibly careful about some things before a new miniskip book. Please do not throw any kind of sharp objects to be had and in case you have complied then please inform particular person who is unloading garbage in advance so they is not hurt any kind of case. Normally you could have to pay some extra amount in this particular case.

Starting a compost bin in your backyard or maybe the neighborhood can definitely help the environment as in order to decomposing your biodegradable waste naturally. Not to mention, not just as good fertilizer for gardens around july decomposed.

By locating your skip conveniently for that work you are doing, it is simple to dispose of the waste because work. This means that you definitely won't be bogged down in broken tiles or ripped up lino for many days on end and in many cases can dispose consultants as when required. Again, this is actually convenient than loading up a van or car several times and traversing to a waste disposal tip.

Don't start with finding a provider to hire from. Identify whether you can need the skip planet first establishment. Understand the task that require to it for and think about if you will any alternatives that gaining control use substitute.

Another best part about skip hire undeniable fact that you are finished with to attend home once they come around to collect the backpack. So if you obtain the job down on a Sunday and require the bin collected on Monday, you are through using to be home. All you need to do is call the company and tell them that are usually done while bag along with they also can come collect this can. Just be sure to end it somewhere a dump tipper may to everything. The tipper will everything clearly and collect it plus your hassle is solved!


text:
KU soccer suffers first setback
The Kansas Jayhawk soccer team lost their first game of the season to the Loyola Chicago Ramblers by a final score of 2-1.
Use the comment form below to begin a discussion about this content.
Commenting has been disabled for this item.
Share this video
Briggs Chrysler 785-856-8889
Find more businesses on Marketplace
Arts & Entertainment · Bars · Theatres · Restaurants · Coffeehouses · Libraries · Antiques · Services

subset: commoncrawl


text:
EDUCAUSE: An overview of E-Portfolios
Individuals and institutions are often asked to provide a record of their accomplishments, whether to show progress in mastering a field or to document educational outcomes. E-portfolios have emerged as a valuable online tool that learners, faculty, and institutions can use to collect, store, update, and share information. E-portfolios allow students to reflect on their learning, communicate with instructors, document credentials, and provide potential employers with examples of their work.... More
Material Type: Reference Material
Date Added to MERLOT: November 28, 2006
Date Modified in MERLOT: January 22, 2015
Submitter: John Ittelson
Reviewed by members of Editorial board for inclusion in MERLOT.Editor Review (not reviewed)
- User Rating (not rated)
- Comments (none)
- Learning Exercises (none)
- Bookmark Collections (none)
- Course ePortfolios (none)
- Accessibility Info (none)
- Rate this material
- Create a learning exercise
- Add accessibility information
Pick a Bookmark Collection or Course ePortfolio to put this material in or scroll to the bottom to create a new Bookmark CollectionName the Bookmark Collection to represent the materials you will addDescribe the Bookmark Collection so other MERLOT users will know what it contains and if it has value for their work or teaching. Other users can copy your Bookmark Collection to their own profile and modify it to save time.Edit the information about the material in this {0}Submitting Bookmarks...Select this link to open drop down to add material EDUCAUSE: An overview of E-Portfolios to your Bookmark Collection or Course ePortfolioSelect this link to close drop down of your Bookmark Collection or Course ePortfolio for material EDUCAUSE: An overview of E-PortfoliosSelect this link to open drop down to add material EDUCAUSE: An overview of E-Portfolios to your Bookmark Collection or Course ePortfolio
Primary Audience: College General Ed, College Lower Division, College Upper Division
Mobile Compatibility: Not specified at this time
Language: English
Cost Involved: unsure
Source Code Available: unsure
Accessibility Information Available: unsure
Copyright: unsure
Creative Commons: unsure
Browse in Disciplines:
Users who viewed this material also viewed...
Discussion for EDUCAUSE: An overview of E-Portfolios
Log in to participate in the discussions or Sign up if you are not already a MERLOT member.


text: A $500 million rail project in California that would transfer cargo inland by rail from Los Angeles and Long Beach ports has been approved by the Los Angeles Harbor Commissioners. Work is slated to start later this year and about 1,500 jobs could be created over the three-year life of the project. "Modernizing our transportation infrastructure is crucial to creating jobs, strengthening our economy and improving our quality of life in Los Angeles," said City Councilman Joe Buscaino.


text:
To embed, copy and paste the code into your website or blog:
Once again using its administrative litigation process, the Federal Trade Commission ("FTC") announced settlements with twelve large businesses, including the Atlanta Falcons and Denver Broncos football teams, the Baker Tilly accounting firm, BitTorrent, Inc., a peer-to-peer file sharing protocol, Level 3 Communications (one of the largest Internet service providers in the world), and Reynolds Consumer Products, all relating to alleged deceptive claims of U.S.-E.U Safe harbor certifications.
The "Safe Harbor" certification, overseen by the U.S. Department of Commerce, is a voluntary privacy certification; however, it requires an annual reaffirmation to maintain "current" certification status. The FTC filed complaints against these companies alleging that the organizations made statements in their privacy policies or displayed the Safe Harbor certification mark indicating that they held current Safe Harbor certifications, even though these companies had allowed their certifications to lapse. The European Commission has recently criticized what it views as lax enforcement of the Safe Harbor process in the U.S., and issued a report with recommendations for improvements. The European Commission will review its participation in the Safe Harbor framework in a decision to be issued by summer 2014.
As background, European Union countries have strict standards regarding the transfer of personal information outside of the EU. Back in 2000, the U.S. Department of Commerce and the European Commission negotiated the U.S.-EU Safe Harbor Framework.. This "Safe Harbor" permits U.S. companies to transfer personal data lawfully from the EU. To participate in the Safe Harbor, a company must self-certify to the Department of Commerce that it complies with seven principles and related requirements that have been deemed to meet the EU's adequacy standard for ensuring the protection of personal data. These principles are: notice, choice, onward transfer (i.e., transfer to third parties), access, security, data integrity, and enforcement. A company's privacy policy must address these principles. The FTC, among other agencies, may enforce Safe Harbor compliance.
The process is entirely voluntary. Once a company self-certifies to the Department of Commerce and Commerce reviews and accepts the filing, a company may state that it has certified compliance with the Safe Harbor. Most companies state this certification in their privacy policies. Organizations may use the Safe Harbor "seal" on their websites and elsewhere. Annually, by the anniversary of its original filing date, a company must "reaffirm" its compliance in order for its certification to remain current.
The FTC's action this week alleges that the twelve companies stated that they held current certifications under the U.S.-E.U. (and in three cases, the similar U.S. –Swiss) Safe Harbor frameworks, when in fact the certifications were not current. Companies which have self-certified compliance with the Safe Harbor framework should check their certifications to ensure they are up-to-date with their annual reaffirmations. The Department of Commerce maintains a public database listing the status of every self-certifying company. While the annual reaffirmation is not an overly taxing task, the FTC's settlements with these companies demonstrate that the agency is taking its Safe Harbor enforcement role seriously and that it is monitoring compliance.
While the proposed settlements do not contain monetary penalties, the companies are barred from any further misrepresentations about their participation in any privacy or data security program sponsored by the government or any other self-regulatory or standard-setting organization. The organizations must also maintain relevant advertisements and promotional materials for five years, and the consent order (once approved) would be in place for 20 years. The proposed settlements are subject to public comment for 30 days and then require final approval of the FTC commissioners.
In privacy law and FTC enforcement, in particular, a guiding principle is "if you say it, do it, and if you don't do it, don't say it." The FTC's action on Safe Harbor enforcement is a good lesson – companies should review their privacy policies to make sure they are up-to-date, accurate, and reflect current practices, including ensuring any certifications are up-to-date. While the U.S.-EU Safe Harbor certification is voluntary, companies must complete their annual reaffirmations on time or risk enforcement.
See more »
DISCLAIMER: Because of the generality of this update, the information provided herein may not be applicable in all situations and should not be acted upon without specific legal advice based on particular situations.
© Jeff Ifrah
Your first step to building a free, personalized, morning email brief covering pertinent authors and topics on JD Supra:
Sign up to create your digest using LinkedIn*
JD Supra provides users with access to its legal industry publishing services (the "Service") through its website (the "Website") as well as through other sources. Our policies with regard to data collection and use of personal information of users of the Service, regardless of the manner in which users access the Service, and visitors to the Website are set forth in this statement ("Policy"). By using the Service, you signify your acceptance of this Policy.
JD Supra collects users' names, companies, titles, e-mail address and industry. JD Supra also tracks the pages that users visit, logs IP addresses and aggregates non-personally identifiable user data and browser type. This data is gathered using cookies and other technologies.
The information and data collected is used to authenticate users and to send notifications relating to the Service, including email alerts to which users have subscribed; to manage the Service and Website, to improve the Service and to customize the user's experience. This information is also provided to the authors of the content to give them insight into their readership and help them to improve their content, so that it is most useful for our users.
JD Supra does not sell, rent or otherwise provide your details to third parties, other than to the authors of the content on JD Supra.
If you prefer not to enable cookies, you may change your browser settings to disable cookies; however, please note that rejecting cookies while visiting the Website may result in certain parts of the Website not operating correctly or as efficiently as if cookies were allowed.
Users who opt in to receive emails may choose to no longer receive e-mail updates and newsletters by selecting the "opt-out of future email" option in the email they receive from JD Supra or in their JD Supra account management screen.
JD Supra takes reasonable precautions to insure that user information is kept private. We restrict access to user information to those individuals who reasonably need access to perform their job functions, such as our third party email service, customer service personnel and technical staff. However, please note that no method of transmitting or storing data is completely secure and we cannot guarantee the security of user information. Unauthorized entry or use, hardware or software failure, and other factors may compromise the security of user information at any time.
If you have reason to believe that your interaction with us is no longer secure, you must immediately notify us of the problem by contacting us at firstname.lastname@example.com. In the unlikely event that we believe that the security of your user information in our possession or control may have been compromised, we may seek to notify you of that development and, if so, will endeavor to do so as promptly as practicable under the circumstances.
Except as otherwise described in this privacy statement, JD Supra will not disclose personal information to any third party unless we believe that disclosure is necessary to: (1) comply with applicable laws; (2) respond to governmental inquiries or requests; (3) comply with valid legal process; (4) protect the rights, privacy, safety or property of JD Supra, users of the Service, Website visitors or the public; (5) permit us to pursue available remedies or limit the damages that we may sustain; and (6) enforce our Terms & Conditions of Use.
In the event there is a change in the corporate structure of JD Supra such as, but not limited to, merger, consolidation, sale, liquidation or transfer of substantial assets, JD Supra may, in its sole discretion, transfer, sell or assign information collected on and through the Service to one or more affiliated or unaffiliated third parties.
This Website and the Service may contain links to other websites. The operator of such other websites may collect information about you, including through cookies or other technologies. If you are using the Service through the Website and link to another site, you will leave the Website and this Policy will not apply to your use of and activity on those other sites. We encourage you to read the legal notices posted on those sites, including their privacy policies. We shall have no responsibility or liability for your visitation to, and the data collection and use practices of, such other sites. This Policy applies solely to the information collected in connection with your use of this Website and does not apply to any practices conducted offline or in connection with any other websites.
We reserve the right to change this Policy at any time. Please refer to the date at the top of this page to determine when this Policy was last revised. Any changes to our privacy policy will become effective upon posting of the revised policy on the Website. By continuing to use the Service or Website following such changes, you will be deemed to have agreed to such changes. If you do not agree with the terms of this Policy, as it may be amended from time to time, in whole or part, please do not continue using the Service or the Website.
If you have any questions about this privacy statement, the practices of this site, your dealings with this Web site, or if you would like to change any of the information you have provided to us, please contact us at: firstname.lastname@example.com.
Already signed up? Log in here
Back to Top
Explore 2016 Readers' Choice Awards
Copyright ©


text:
Ministry of Defence working arrangements with media organisations (MOD green book)
A guide to UK Ministry of Defence procedure for working with the media.
This document covers the practical arrangements for enabling correspondents to report on operations, including the Ministry of Defence's plans for representative numbers of correspondents to accompany British forces. It also sets out what editors can expect from the Ministry of Defence (MOD) and what the MOD seeks from the media.


text:
Earth Day 2016
Friday, April 22, 2016
Join us as we revisit one of our stops on the Urban Plunge– Good Life Growing urban farm in N. St. Louis City! We'll help out at the farm and contribute to the food justice and sustainability movement in North City.
Meet at Newman at 9:30 am to travel to the farm– lunch is on us! We'll be back around 3 pm. Or, meet us at the farm anytime– 4057 Evans. Ave.
To learn more about the farm, read this article.
Contact Erin to sign up: firstname.lastname@example.com.
The Newman Center strives to serve our local community and to open the eyes of our students' to the needs of those around them. We volunteer almost every month (usually on Friday because most students don't have classes that day) with various organizations around the St. Louis Metro Area. If you would like to be placed on our Service Friday e-mail list, send your name and e-mail address to firstname.lastname@example.com. If you join the e-mail list, we will alert you to upcoming service opportunities a few weeks before the event.
For upcoming Service Friday events, email our Officer of Service and Justice, Janelle Miller: email@example.com


text:
A commercial airliner crashed into a densely populated neighborhood in Nigeria's largest city on Sunday, killing all 153 people on board and others on the ground in the country's worst air disaster in nearly two decades.
Comment count on this article reflects comments made on Breitbart.com and Facebook. Visit Breitbart's Facebook Page.


text:
A rather persistant guy was trying to talk me into playing an ELO format match against him for a big bet. I don't knwo the guy but he was offering to bet a Guru Cr and a Kiki Cr against my 500 000 or Dwain Cr. He claimed he won them on a WoP. What would that be?
He kept trying to talk me into it, and I only got him to back off when I asked him to put them on the market so I can see them. Should I report him befort he scams someone or what?
So, err, why not report him if we all know he's a scammer?
And the secure trades thing has been not working for a while now, wonder whats up with that... Anyone seen any official info or?
To be honest,i do not know why he hasnt been reported yet...
Not breaking any rules i dont believe if its a mutual agreement between two players,
chances are you will lose your cards if you agree to play for ante with this guy, better off not taking the risk.
Wow, I was gonna ask if it was Josh as a joke. Can't believe it really was him.
Anyways, WOPs aren't something moderators can enforce. They're not against it, but they're not for it (I think). I bet you he's been reported MANY times before, and while I'm sure some moderators have their personal opinions on actions that should be taken against him, they can't actually intervene in WOP business.
But I do gotta clarify and say what he's doing isn't a scam. Not by definition.
Should you happen to WOP Josh and lose to him, and not pay him, no one will hold it against you. He just clearly doesn't care if ppl hate him. Also he made a lotto 2 days ago or so claiming he'd change, so thanks for bringing it to our attention that he has NOT changed
They can make betting a mode, therefore preventing scams (people will do it, so they might as well try to protect the people doing it?). Offer cards up for trade, then both players must agree what they are betting and a selected format (ELO, standard 25, or whatever format). The cards and/or clintz are put into a pool and winner takes all.
The people will then be able to know if a player really is betting, and they know the risk ('cuz the card will leave their collection and only come back if they win).


text:
Map of Musical Events in Switzerland
Browse 797 attractions, meet 87 travelers, 37 tour guides and discover 2,620 photos
A map of musical events in Switzerland. TouristLink also features a map of all the musical events in Europe. Want more information? Check out this list of top ranking musical events in Switzerland
Check out our photos of "Musical Events in Switzerland." Click a photo of a musical events to see its location on the map and find out more information. We have photos of all 787 tourist attractions around Switzerland or add your own (Click here to browse them).


text:
Autumn Statement: Public reaction to austerity measures
5 December 2012 Last updated at 19:50 GMT
There has been a mixed reaction to the chancellor's announcement that at least six more years of austerity measures lie ahead for those in the UK.
In his annual Autumn Statement, George Osborne unveiled a raft of measures which included the cancellation of a planned fuel duty rise and a reduction in the lifetime pension relief allowance.
Reeta Chakrabarti spoke to drivers, families and businesses in Birmingham.


text:
I say follow your feelings and be very weary!My 35 year old BIL has shown an 'unhealthy' interest in my kids (and their cousins). He pushes normal social boundaries, kissing them constantly, offers to bath them, always wants to take the kids to the park alone, and always has to carry my 2 year old DD rather than letting her walk. When we're leaving he says "kiss on the lips for uncle ....". It makes me want to vomit!
Every time we have a family get-together people always comment "oh, he's so good with kids isn't he?".
I have no evidence that he's a pedophile but you can understand my concern. The way he acts around the kids is just not normal for a male that age.
He has a history of major drug use. Supposedly he is now clean but the drugs have definately left some permanent brain damage... he often says inappropriate things in social situations, etc. So there's a chance that he is not a pedophile at all and simply doesn't understand normal boundaries with children. But I'm not prepared to take that risk.
He is currently living at my in-law's so we can't avoid seeing him. I'm sick of following him around everywhere to keep an eye on my kids. And I can't let my in-laws babysit because I can't trust that they won't leave my kids alone with him.
I know I need to talk to my DH about it. I have made a couple of comments to which he just says I'm over-reacting.
Has anyone else been in a similar situation where you didn't trust someone around your kids? Any advice?


text:
Is INSTANCE SOFTECH PVT LTD incorrect or missing key business information? Update your Hotfrog listing today.
Contact details
Are these details correct?
Instance Softech is an Indian IT Consulting Company with 40+ professionals having its corporate office in . We primarily focus on
• Staff Augmentation (Permanent & Contract)
• Software Solutions
• IIT & REC Profiles
A unique blend of corporate values, enthusiastic, skilled and experienced staff, ISO 9001 compliant quality processes and state of the art technology are the key differentiation factors in providing best of breed solutions and Services to customers across the globe.
We have an excellent team of professionals who have joined hands with us not just for 'what we are', but for the reason 'what they can be with us' in future. At Instance Softech, we have found that Exceptional people skills make the difference between customer Satisfaction and client delight Our resources have a vast global experience and proven track record whose skills are honed through a series of intensive training programs and exposure to the most cutting edge technologies and tools under the guidance of, Industry Certified Architects. Our development centre resources are servicing leading IT companies.
Instance Softech relationships with clients are enduring and always mutually beneficial. The purpose of this mail is to explore mutual business opportunities along these lines. We would appreciate if you provide us an opportunity to serve you and we ensure that Instance Softech become a value-addition to your concern.
Is INSTANCE SOFTECH PVT LTD in #19,1st Floor, 2nd Cross, BHCS Layout, BTM 2nd Stage, Bangalure Karnataka 560076 your business? Claim your listing and attract more leads by adding more content, photos and other business details.
We have more Software Development services in Bangalure Karnataka available on Hotfrog INDIA. You can update your search for Bangalure Software Development by location, keyword or service options.
Small Business Hub
Sharing tips, ideas and advice to help grow your small business.
Your profile without ads
Have you considered upgrading to Hotfrog AdVantage? No competitor advertising means exclusive focus on your business. You take centre stage.
Create your free Hotfrog business profile
Follow Hotfrog on:
Share on:
Hotfrog Small Business Hub


text: Summer En France (featuring Naadei) (PREVIEW) by LIL JON published on 2012-06-15T20:21:10Z NEW SINGLE PRODUCED BY GREGOR SALTO Genre Dance Comment by Crow A Sekas Fuck Yeah!! 2014/01/30 13:13:12 +0000 Comment by Pablo de jesus ❤ 2013/10/26 16:42:05 +0000 Comment by MentallyCrunk i miss the summer but this is a nice summer song lil jon 2013/01/27 17:56:56 +0000 Comment by Packkman Beats YOU WENT IN ON THIS BRO! 2012/10/16 15:57:04 +0000 Comment by DJ Flipster yo where can i get a hold of this? 2012/06/18 03:16:41 +0000 Comment by FuegoTheDJ Sick track! hit me up when your in SAN DIEGO! For real! 2012/06/17 23:06:32 +0000 Comment by djmyster hot single' 2012/06/17 20:15:20 +0000 Comment by Marcos Crunk Very Good Lil Jon You're even the king ! ! ! 2012/06/17 19:31:50 +0000 Comment by unKnown Conflict would love the remix stems!!! this is gonna be a dope song 2012/06/17 14:46:15 +0000 Comment by Perezance It has to grow on me like every song, but this is definitely a song for the summer and for any time you feel down. PARTYYY!!! :D 2012/06/17 00:05:18 +0000 Comment by DJ Mr.Chen/Bangerz i hope see you in beijing spark again :) 2012/06/16 20:46:49 +0000 Comment by djflacoflash this will most def work! 2012/06/16 19:56:39 +0000


text:
There's a brand-new Acura MDX on its way, but if you have been following CarScoop on a regular basis, you probably already know that as we have shared with you spy photos of the three-row crossover.
What you may have not seen yet is the camouflaged prototype of the Acura model on film and on the move.
The 2014 MDX was recorded on camera as it was exiting Acura's test facility in Germany, followed by a current generation of the MDX and a BMW X5, no doubt for comparison purposes as the two luxury crossovers are similar in size and even closely priced in their base trims in the States.
Acura's redesigned proposal in the premium crossover segment will have a somewhat crisper exterior styling and offer improved interior space thanks to a longer body.
It is possible that Acura will offer the next MDX with Honda's updated 310hp V6 as well as a hybrid powertrain matching a 3.5-liter direct-injected VTEC V6 to a 7-speed dual clutch transmission and up to three electric motors.
The estimated time of arrival for the 2014MY MDX is the second half of next year.
Video Credits: Carparazzi/YouTube


text:
The man suspected of hijacking an EgyptAir flight and redirecting it to Cyprus has appeared in court and said he acted in a bid "to see his wife and children".
Seif Eldin Mustafa, 59, flashed "v" signs for victory as he left the court in Larnaca following a short hearing, where he was remanded in custody for eight days on suspicion of hijacking, abduction, threatening violence, terrorism-related offences and two counts related to possession of explosives.
Police said the latter charges would relate to Mr Mustafa's alleged claim that he had a bomb vest, even though Cypriot officials later said the "explosives" were in fact iPhone covers bound together with cloth.
It has previously been reported that the hijacker made a range of demands after the plane from Alexandria landed at Larnaca airport with 72 passengers and crew on board.
After a six-hour stand-off, the suspect identified by Egyptian and Cypriot authorities as Mr Mustafa walked off the plane and handed himself in to police.
In a statement to Cypriot police, Mr Mustafa said: "When someone hasn't seen his family for 24 years and wants to see his wife and children, and the Egyptian government doesn't allow it, what should one do?"
The suspect allegedly commandeered the aircraft 15 minutes after takeoff from Alexandria. He approached a flight attendant and showed off the belt, attached to a remote control he held in his hand, investigating officer Andreas Lambrianou told the court.
"The suspect asked all passengers and crew to hand in their passports, then gave two messages to a member of the crew, asking that the pilot be informed that he was a hijacker and wanted to land at an airport in Turkey, Greece or Cyprus, but preferably Cyprus," Lambrianou said.
"In a note, he stressed that if the airplane landed on Egyptian territory he would immediately blow the plane up."
In pictures: EgyptAir hijacked plane
In pictures: EgyptAir hijacked plane
Seif Eldin Mustafa, suspected of hijacking EgyptAir plane MS181, flashes the 'V' for victory sign as he leaves the court in Larnaca in a police car
AFP/Getty Images
Seif Eldin Mustafa wears handcuffs as he leaves the court in Larnaca escorted by Cypriot police
AFP/Getty Images
An EgyptAir flight 181 passenger embraces a family member after arriving at Cairo international airport
CCTV footage shows Seif Eldin Mustafa walking through a metal detector before being patted down by security
Egypt Ministry of Interior
Cypriot police guard the hijacked EgyptAir A320 plane at Larnaca Airport after it landed in Cyprus
Passengers evacuate a hijacked EgyptAir Airbus 320 plane at Larnaca airport, Cyprus
An official boards a hijacked Egyptair A320 Airbus at Larnaca Airport in Larnaca, Cyprus
An Egypt Air Airbus A-320 sits on the tarmac of Larnaca aiport after it was hijacked and diverted to Cyprus
A Cypriot policeman stands guard near a hijacked EgyptAir A320 plane at Larnaca Airport, Cyprus
Screen grabbed image taken from Flightradar24 of EgyptAir plane MS181 which has landed in Cyprus after being hijacked while flying from Alexandria to Cairo
In Cyprus, Mustafa dropped an envelope on the runway addressed to a Cypriot woman, later ascertained to be his ex-wife. In the letter, the suspect demanded the release of 63 female prisoners held in Egypt.
Additional reporting by Reuters
- More about:
- EgyptAir hijacking


text:
EuroMillions checker
Check Your Numbers Instantly!
Use the automatic EuroMillions Results Checker to quickly check your tickets and see if you're a winner. Simply enter your winning numbers into the EuroMillions number checker and press the "Check Numbers" button and the Checker will instantly scan all possible winning combinations to see if you've won a prize.
Check Old EuroMillions Tickets
Did you find an old EuroMillions ticket? All of the past EuroMillions winning numbers are stored in the Results Checker. Just remember that if you bought your ticket offline you only have 180 days to claim your prize in the UK.
EuroMillions Winning Numbers
Do you have the EuroMillions winning numbers? You may be able to claim direct from the retailer. The amount you can claim in-store varies from country to country while for larger prizes you will need to contact your national lottery provider. Don't forget, also, that, for added convenience, you can also play EuroMillions online and get instant payments on wins.
Prizes must be claimed within 180 days, unless the ticket was purchased online.


text:
B3a Molecules of Life Answer
B3a Molecules of Life Answer Sheet
Teacher recommended
- Created by: Amira
- Created on: 02-05-12 09:12
First 191 words of the document:
B3a Molecules of Life
1. Nucleus carries genetic information.
Cell membrane controls the movement of substances in and out of cells.
Cytoplasm is where many chemical reactions happen.
Respiration occurs in the mitochondria providing energy for life processes.
2. Label these parts on the animal cell
Cell membrane / mitochondria /
nucleus / cytoplasm
Higher only
2. Pepsin is an enzyme that digests (breaks down) protein.
Pepsin is produced in the stomach.
(a) Plot a graph using her first three results.
Finish the graph by drawing the best curve.
Answer: A smooth curve no flat line at the top.
(b) What results would you expect at pH 4 and pH 5?
Extend the line to show your answer.
Answer: The line then should come down.
(c) Protease
3. (a) Many chemical reactions in cells are controlled by enzymes.
(i) A smooth curve no flatline at the top.
all points correctly plotted to within +/ 0.5 square (1)
smooth line of best fit (1)
(ii) Answers between 7 to 9
(b) Amylase denatures / active site changed
(c ) denatures
Other pages in this set
Page 2
Here's a taster:
Genes are made of DNA.
(i) The diagram shows DNA replication (copying).
Complete the diagram to show the bases in the new DNA strands
(ii) describe the stage is involved in the production of human insulin, by genetically
modified bacteria
Human gene that produces insulin is removed
This gene is inserted into the chromosome of a bacteria
These chromosomes are called plasmids
Then the bacteria are reproduced in a fermenter.…read more


text:
We can provide you complete Reseller panel to start your own VOIP calling card business without any investment in Hardware, software....
Our services is working in all Middle East Countries : Oman, Yemen, UAE, Kuwait Qatar, Bahrain ,Saudi Arabia....
The services include:
• Reseller Module
• Mobile Dialer
• PC-to-Phone
• Device-to-Phone etc..
• Very simple and Friendly Web Management Interface.
• You can set your own Rates, and determine your own Profit
• Very High Quality Routes
Best quality White Cli routes....
You can pay IN INDIA or in UAE
Start business with very low cost.......
Contact me for further details.
MSN : email@example.com
Gmail: firstname.lastname@example.com
Yahoo: firstname.lastname@example.com


text:
SGS Distributor alleges Cheating by Pawan Kalyan Team
Sampath Kumar who distributed Sardaar Gabbar Singh in Krishna District... Read more →
Sampath Kumar who distributed Sardaar Gabbar Singh in Krishna District... Read more →
Creative Director Sukumar and Music Devil Devi Sri Prasad are... Read more →
Megastar Chiranjeevi's Prestigious 150th film, Khaidi No 150 has opened... Read more →
Young Hero Sharwanand's Shatamanam Bhavathi released the other day. The... Read more →
Natural Star Nani's 'Nenu Local' is unstoppable at the box-office.... Read more →
Natural Star Nani's Nenu Local Theatrical Trailer is rocking in... Read more →
Rana Daggubati's Ghazi is going strength to strength at the... Read more →
Superstar Mahesh Babu's next in the direction of Tamil Star... Read more →
Powerstar Pawan Kalyan's Katamarayudu is likely to have no audio... Read more →
Natural Star Nani's Nenu Local is all set for release... Read more →
. Tapsee Hot Photos in Shadow Movie,Shadow Movie Heroine Tapsee hot pics,telugu movie Shadow actress tapsi stills,venkatesh,Srikanth Shadow movie photos... Read more →
. It's only fair to share... comments Read more →
Dimple Chopra Photos..Tollywood Actress Dimple Chopra Photos..Dimple Chopra | Dimple Chopra Cute photos | Dimple Chopra hot photos |Dimple Chopra... Read more →
. It's only fair to share... comments Read more →
Namitha hot Photos from her latest movie,Namitha Hot in Short Dress Photos,Namitha Hot Spicy Photos,Actress Namitha Hot pics It's only... Read more →
. Katherine Theresa Hot Images,Iddarammayilatho Heroine Katherine Theresa Hot Photos,Katherine Theresa Hot thigh show photos,Katherine Theresa It's only fair to... Read more →
. Anushka Hot Photos in Mirchi Movie,Mirchi Heroine Anushka shetty Photos,Anushka In modern Dress,Anushka In Saree in Mirchi Movie It's... Read more →
Richa Gangopadhyay Hot Photos in Mirchi Movie,Mirchi Heroine Richa Gangopadhyay Photos,Richa Gangopadhyay In modernd Dress,Richa Gangopadhyay Hot stills It's only... Read more →


text:
This Lent, take a 40-day devotional journey with Dietrich Bonhoeffer. Click here to sign up.
6:2 days may be long. These words translate the same Hebrew phrase as in 5:33. Here also we may understand the meaning as "long life in the land."
Starting your free trial of Bible Gateway Plus is easy. You're already logged in with your Bible Gateway account. The next step is to enter your payment information. Your credit card won't be charged until the trial period is over. You can cancel anytime during the trial period.
Click the button below to continue.
You've already claimed your free trial of Bible Gateway Plus. To subscribe at our regular subscription rate of $3.99/month, click the button below.
It looks like you're already subscribed to Bible Gateway Plus! To manage your subscription, visit your Bible Gateway account settings.
Try Bible Gateway Plus, a brand-new service that lets you experience Bible Gateway free of banner ads! It also gives you instant access to over 40 Bible study and inspirational devotional books, including the NIV Study Bible. With Bible Gateway Plus, you can experience and understand God's Word in life-changing new ways, without the distraction of ads. Try it free for 30 days—you can cancel at any time. Following your 30-day free trial, Bible Gateway Plus is only $3.99/month.
Three easy steps to start your free trial subscription to Bible Gateway Plus.


text:
Purchase Options
Contains 3 essential MCAT tests needed for your study:
Real ToolFor the mcat writing sample, I thank you. It really gave me the real touch of MCAT exam. I am so happy because I passed my test. I want to thank everyone at test-inside for your sample mcat questions. I used other tools and sample questions as well, but you gave me the real thing. I noticed that your mcat sample questions were very similar to the real questions of test. Thank you so much for the help. I will definitely be using test-inside again for my future studies. Robin
Special Thanks To YouI want to say a special thank you for the tremendous writing sample mcat that you provided me for practice. I could never able to pass the mcat writing samples without your sample mcat essays. I was very much benefited by that. Thank you so much for giving us opportunity to have these questions. I am so happy that I used test-inside, you really helped me to pass the MCAT exam. My credit of success all goes to test-inside. Anon Boyer
Wanted To Let You KnowI just wanted to let you know that I passed my MCAT test. You made an incredible mcat sample essays. I passed my mcat sample papers with high scores. I am very happy to have high scores in test. Thank you so much for providing me such great mcat samples. You are truly the best. Your questions and content really helped me to get high scores in MCAT test. You built my confidence through your content, explanations and questions. Thanks a million Juan Chery
I Am So GladI am so glad that I passed my MCAT test! Your mcat sample essays made the test easier for me! Your mcat sample papers helped me to understand the stuff of the MCAT. Your mcat samples played a major role in my preparation of the MCAT test! I am happy that I chose the correct path of subscribing to test-inside. I will definitely use your services for other exams! I highly appreciate your services and I will recommend it to others too. Thanks and regards, Mike Alexander
Really Thankful For Your Mcat Sample EssaysI used the mcat sample papers before the MCAT actual exam. And I am really thankful to you for providing such a helpful mcat samples , that really gave me a virtual image of the MCAT . I am now MCAT and I am so excited. Test-inside led me to the right way from my early start. It was very useful to get familiar with the MCAT exam, and of course it helped me to get my MCAT Certificate. After my experience, I just recommend your free MCAT test to all! Charlene Joan
Experience Test-inside testing engine for yourself.
Simply submit your e-mail address below to get started with our interactive software demo of your MCAT test.
Customizable, interactive MCAT testing engine
Simulates real MCAT test environment
Instant download


text:
Effects of cyclin D1 gene amplification and protein expression on time to recurrence in postmenopausal breast cancer patients treated with anastrozole or tamoxifen: a TransATAC study
DOI: 10.1186/bcr3161
© Lundgren et al.; licensee BioMed Central Ltd. 2012
Received: 5 August 2011
Accepted: 4 April 2012
Published: 4 April 2012
Gene amplification of CCND1 is observed in a subgroup of breast cancers with poor prognosis, whereas overexpression of the protein cyclin D1 has been linked to both worse and better clinical outcome. CCND1 amplification and protein overexpression have also been associated with resistance to treatment with tamoxifen or even to a potentially detrimental effect of tamoxifen.
To clarify these challenging and partly contrasting treatment predictive and prognostic links for cyclin D1 we analysed a large cohort of postmenopausal breast cancer patients randomised to receive either adjuvant anastrozole or tamoxifen, as part of the Arimidex, Tamoxifen, Alone or in Combination (ATAC) trial. The CCND1 amplification status and protein expression of cyclin D1 were assessed by chromogenic in situ hybridisation and immunohistochemistry, respectively, in 1,155 postmenopausal, oestrogen-receptor-positive breast cancer patients included in the TransATAC substudy.
Amplification of CCND1 was observed in 8.7% of the tumours and was associated with increased risk of disease recurrence (hazard ratio = 1.61; 95% confidence interval, 1.08 to 2.41) after adjustment for other clinicopathological parameters. In contrast, nuclear expression of cyclin D1 protein was associated with decreased recurrence rate (hazard ratio = 0.6; 95% confidence interval, 0.39 to 0.92). The intensity of nuclear or cytoplasmic expression was not of prognostic value. There was no significant interaction between cyclin D1 status and treatment efficacy, ruling out any major detrimental effect of tamoxifen in CCND1-amplified postmenopausal breast cancer.
In summary, CCND1 amplification and low nuclear expression of cyclin D1 predicted poor clinical outcome in postmenopausal breast cancer patients treated with either anastrozole or tamoxifen.
Trial Registration
Current Controlled Trials ISRCTN18233230.
Hormone dependence is a fundamental hallmark of the majority of breast cancers, and tumour growth can be inhibited either by deprivation of circulating oestrogens or by antagonising the effect of these hormones on their receptors [1]. The selective oestrogen receptor (ER) modulator tamoxifen has long been the most commonly used adjuvant therapy for patients with advanced hormone-sensitive breast cancer [2]. In recent years, however, aromatase inhibitors have become an alternative treatment option for postmenopausal women with breast cancer. An aromatase inhibitor acts by interfering with the enzyme that converts androgens to oestrogen, and reduces tumour and systemic oestrogen concentration [3]. The third-generation selective aromatase inhibitor anastrozole (Arimidex) reduces serum oestradiol to nanomolar concentrations [4]. The Arimidex, Tamoxifen, Alone or in Combination (ATAC) trial was designed to compare the efficacy of anastrozole alone or in combination with the established adjuvant treatment, tamoxifen for 5 years, as adjuvant treatment for postmenopausal women with operable breast cancer [5]. The study demonstrated that the efficacy of anastrozole was higher compared with tamoxifen alone, and also superior to the combination of both agents [5, 6]. After a median follow-up of 10 years, 5 years after completion of treatment, the significant advantage for anastrozole over tamoxifen as initial adjuvant therapy for postmenopausal, ER-positive breast cancer patients was confirmed [7].
In breast cancer, genetic alterations such as amplifications and deletions occur within the tumour at high frequencies, and a number of these alterations are closely related to poor clinical outcome. One such region of amplification is 11q13, harbouring the cyclin D1 gene CCND1 [8–10]. Cyclin D1 plays a crucial role as a cell cycle regulator, promoting progression through the G1-S phase, following complex formation with CDK4/6 and phosphorylation of the retinoblastoma protein [11]. Various studies have described the oncogenic capacity of cyclin D1 in vitro, and overexpression in vivo results in tumour formation [12–14]. Overexpression of cyclin D1 is observed in approximately 50% of breast cancers [15, 16], and cyclin D1 is one of the most commonly overexpressed proteins in this form of cancer. A number of studies report cyclin D1 overexpression to be a predictor of worse prognosis [17, 18], while others have found an association with an ER-positive phenotype and a better clinical outcome [19–23]. In about 15% of all primary breast cancers, overexpression is due to amplification of the corresponding gene CCND1 [15, 24, 25], and this specific amplification has been linked to poor prognosis [23, 26].
Despite the presence of ERα, approximately 50% of breast cancers develop resistance to hormonal treatment, a major clinical limitation of breast cancer therapy [27, 28]. The mechanisms behind this phenomenon have been extensively studied, and imply a complex signalling network governing ER function and interaction with various co-regulators [29–32]. Cyclin D1 is one such co-factor, known to interact with ERα and, independently of oestrogen, activate the receptor and potentially modify oestrogen/anti-oestrogen responses [33, 34]. Overexpression of cyclin D1 has been reported to result in a conformational change in ERα that induces receptor activation in the presence of the novel selective ER modulator arzoxifene, which in turn promotes growth of MCF-7 cells - indicating a change from antagonist to agonist [28]. This study also suggests that different mechanisms are required to confer resistance depending on the specific anti-oestrogen administered, and that changes in the conformation of ERα play a crucial role in anti-hormonal insensitivity. A similar study demonstrated that overexpression of cyclin D1 reversed the growth inhibitory effect of tamoxifen in two ER-positive breast cancer cell lines [35]. In line with these experimental findings we have previously observed that cyclin D1 overexpression was associated with tamoxifen resistance in premenopausal and postmenopausal breast cancer [21, 36]. Worryingly, amplification of CCND1 was further linked to a potentially detrimental effect of tamoxifen in premenopausal breast cancer patients, when compared with randomised control patients not receiving any adjuvant therapy [36].
The aim of our study was to characterise the association between CCND1 amplification and cyclin D1 protein expression and breast cancer recurrence in a large randomised cohort of postmenopausal patients with ER-positive breast cancer treated with endocrine therapy. In addition, we aimed to assess whether there was a significant difference in response to anastrozole versus tamoxifen according to cyclin D1 gene and protein status, and thereby to address any potentially unfavourable effects of tamoxifen in subgroups of breast cancer defined by CCND1 amplification.
Materials and methods
The ATAC trial originally evaluated the efficacy and safety of 5 years of anastrozole, tamoxifen, or the combination of both treatments in postmenopausal patients presenting with localised breast cancer [7]. For the TransATAC protocol, formalin-fixed, paraffin-embedded blocks of the primary tumour were collected from as many hormone-receptor-positive patients as possible, from the monotherapy trial arms [37]. The endpoint for the analyses was any breast cancer recurrence and the median follow-up time was 10 years. The original study was performed according to the Declaration of Helsinki after approval by an institutional review board and ethics committee, and informed consent was obtained from all patients enrolled in the study.
Nine tissue microarrays (TMAs) were used, each originally including from 165 to 200 tumour tissue samples from the patients included in the TransATAC study. This set of TMAs had one tissue core for each patient: a sample set was analysed for cyclin D1 protein expression and a set was analysed for CCND1 copy number. For detailed description of the TMA assembly we refer to our previously published study [37]. The TMA slides were deparaffinised, rehydrated and microwave-treated in target retrieval solution pH 9.9 (Dako, Glostrup, Denmark), and were processed in an automated immunostainer (Techmate 500; Dako, Copenhagen, Denmark) using the Envision software (Dako, Glostrup, Denmark). The antibody employed was a mouse monoclonal antibody reactive against human cyclin D1 (1:100, clone DSC-6; Dako, Glostrup, Denmark).
Staining of cyclin D1 was assessed as cytoplasmic staining intensity (0 to 2) as well as nuclear staining intensity (0 to 3) and fraction-positive nuclei (0, < 1%, 1 to 9%, 10 to 32%, 33 to 67% and > 67%) according to the Allred Score [38]. Evaluation was performed by two independent observers (one a pathologist), with the pathologist's score superseding the other observer's at consolidation. Conflicting observations were low (< 5%) for all evaluations made. All immunohistochemical evaluations were performed without knowledge of tumour characteristics. In cases of no evaluation, the tumour cores were either nonrepresentative (that is, no invasive tumour cells) or were missing. This study was carried out and is reported according to REMARK guidelines [39].
Chromogenic in situ hybridisation
Chromogenic in situ hybridisation (CISH) was performed according to the Zymed SPoT-Light Cyclin D1 Probe protocol suited for CISH [40] using the SPoT-Light Cyclin D1 Amplification Probe (Zymed Laboratories, Invitrogen Immunodetection, San Francisco, CA, USA). Pretreatment procedures included heating and enzyme digestion to optimise the CISH performance. Nonamplified cases were classified as 0, cases with up to 8 copies classified as 1 and > 8 copies classified as 2. In statistical analyses, classifications 1 and 2 were both included in the subgroup defined as amplified.
Statistical analyses
The primary endpoint for the analyses was time to recurrence (TTR), also known as the recurrence-free interval. TTR was defined as the time from randomisation to first locoregional recurrence, distant recurrence or contralateral disease. Statistical analyses were performed according to a prespecified statistical analysis plan approved by the ATAC Steering Committee. Cox proportional hazards regression models were fitted to TTR, and hazard ratios (HRs) and associated 95% confidence intervals (CIs) were estimated. The statistical tests employed for correlations between cyclin D1 variables and clinicopathological parameters were the Armitage's trend test, the Wilcoxon test, the Goodman's test and the Cuzick test [41]. Multiple hypothesis testing was not corrected for, thus marginal P values should be interpreted with caution. The contribution of cyclin D1 protein expression was analysed by the change in likelihood ratio chi-squared test (one degree of freedom) univariately and multivariately, in addition to a model with tumour size, nodal status, grade (central) and Ki67 expression, for all patients and nonamplified patients. All hypothesis tests were conducted at the two-sided P = 0.05 level. For detailed description of statistical analyses, we refer to a previous report [42].
Distribution of CCND1 amplification status and cyclin D1 protein staining categories
Associations between CCND1 amplification status, cyclin D1 protein expression and clinicopathological variables in all patients
All patients
Cyclin D1 cytoplasmic intensity (negative/low, intermediate, high)
Cyclin D1 nuclear intensity (negative/low, intermediate, high)
Cyclin D1 nuclear fraction (< 1%,1 to 9%, 10 to 32%, 33 to 67%, > 67%)
Tumour grade (well, moderate, poor)
Tumour sizea (mm)
Nodal status (negative, 1 to 3, > 3)
CCND1 amplification
12%, 52%, 36%
40%, 36%, 24%
10%, 24%, 27%, 24%, 15%
23%, 61%, 16%
70%, 23%, 7%
6%, 48%, 46%
9%, 35%, 56%
0%, 6%, 18%, 32%, 44%
9%, 62%, 29%
64%, 26%, 10%
Armitage (P = 0.063)
Armitage (P < 0.001)
Armitage (P < 0.001)
Armitage (P < 0.001)
Wilcoxon (P = 0.083)
Armitage (P = 0.341)
Wilcoxon (P < 0.001)
Cyclin D1 cytoplasmic intensity
74%, 16%, 10%
23%, 33%, 26%, 11%, 4%
26%, 59%, 15%
68%, 24%, 8%
40%, 36%, 24%
10%, 24%, 27%, 25%, 14%
17%, 63%, 20%
64%, 34%, 2%
20%, 43%, 37%
3%, 16%, 25%, 28%, 28%
23%, 61%, 16%
74%, 20%, 6%
Goodman = 0.44 (P < 0.001)
Goodman = 0.39 (P < 0.001)
Goodman = -0.06 (P = 0.238)
Cuzick trend (P = 0.010)
Goodman = -0.12 (P = 0.031)
Cuzick trend (P = 0.021)
Cyclin D1 nuclear intensity
23%, 33%, 26%, 11%, 4%
24%, 62%, 14%
67%, 25%, 8%
1%, 12%, 41%, 36%, 10%
18%, 65%, 17%
68%, 25%, 7%
0%, 1%, 7%, 37%, 55%
17%, 59%, 24%
71%, 22%, 7%
Goodman = 0.88 (P < 0.001)
Goodman = 0.16 (P < 0.001)
Cuzick trend (P = 0.553)
Goodman = -0.05 (P = 0.327)
Cuzick trend (P < 0.001)
Cyclin D1 nuclear fraction
< 1%
18%, 68%, 14%
2%, 29%, 9%
1 to 9%
23%, 62%, 15%
68%, 25%, 7%
10 to 32%
24%, 59%, 17%
71%, 25%, 4%
33 to 67%
20%, 59%, 21%
70%, 21%, 9%
> 67%
12%, 68%, 20%
68%, 24%, 8%
Cuzick trend (P = 0.005)
Spearman ρ = -0.04 (P = 0.162)
Cuzick trend (P = 0.694)
Spearman ρ = 0.22 (P < 0.001
CCND1 gene amplification and patient prognosis
Cox proportional Hazards models for estimating the effect on time to recurrence
All patients
CCND1, amplified vs. nonamplified
2.04 (1.37 to 3.03)
< 0.001
1.61 (1.08 to 2.41)
Cyclin D1 cytoplasmic intensity, high vs. remainder
0.71 (0.51 to 0.98)
0.78 (0.56 to 1.09)
Cyclin D1 nuclear intensity, high vs. remainder
0.91 (0.64 to 1.28)
0.85 (0.59 to 1.21)
Cyclin D1 nuclear fraction, 1 to 100% vs. < 1%
0.58 (0.38 to 0.90)
0.60 (0.39 to 0.92)
Nonamplified patients
Cyclin D1 cytoplasmic intensity, high vs. remainder
0.57 (0.39 to 0.82)
0.64 (0.44 to 0.95)
Cyclin D1 nuclear intensity, high vs. remainder
0.88 (0.60 to 1.32)
0.84 (0.55 to 1.26)
Cyclin D1 nuclear fraction, 1 to 100% vs. < 1%
0.52 (0.34 to 0.81)
0.54 (0.35 to 0.85)
Cyclin D1 protein expression and patient prognosis
Interactions of cyclin D1 and treatment, nodal status and Ki67
Cyclin D1, CCND1 and clinicopathological data
The CCND1 amplification status was positively correlated to tumour grade (P < 0.001) and proliferation (defined as Ki67 expression) (P < 0.001), but not to nodal status or tumour size (Table 1). Cytoplasmic cyclin D1 expression was inversely correlated to tumour size (all patients; P = 0.01) and nodal status (P = 0.031), and was positively correlated to proliferation (P = 0.021). Both nuclear staining intensity of cyclin D1 and fraction-positive nuclei were associated with higher grade (P < 0.001 and P = 0.005 respectively) and higher proliferation rate (both P < 0.001).
Cyclin D1, proliferation and time to recurrence
Amplification of the CCND1 gene has been associated with a poor patient outcome in previous studies [19, 26], whilst controversy regarding overexpression of cyclin D1 protein in relation to patient survival still exists. Cyclin D1 has been reported to be a prognostic marker in invasive breast cancer and has been associated with both a less aggressive ER-positive phenotype [20, 22] and also with an adverse clinical outcome [18]. These conflicting findings can potentially be explained by the low patient numbers analysed and/or methodological discrepancies. To clarify the importance of cyclin D1 in breast cancer we therefore analysed the expression of cyclin D1 in different subcellular localisations, using a previously validated antibody [36], as well as the gene amplification status by the well-established CISH technique in a large, well-characterised randomised patient cohort including more than 1,000 patients with ER-positive breast cancers. Our data support studies indicating that low cyclin D1 protein expression as well as CCND1 amplification are linked to tumour aggressiveness and increased risk of disease recurrence in ER-positive postmenopausal breast cancer [23, 26]. Similar findings have been observed for HER2, where both high expression linked to amplification and low expression are linked to poor outcome [43].
Amplification of CCND1 was observed in 8.7% of the tumours, which is slightly lower than the frequency of 10 to 15% generally reported, even though some groups have demonstrated a lower percentage of CCND1-amplified tumours [15, 44, 45]. The slightly lower fraction of CCND1-amplified cases may be due to all patients being ER-positive or due to methodological differences and different cutoff points for defining amplification between studies. In addition, the use of TMAs has certain limitations; however, this technique is indispensable when analysing large patient materials and is today a well-accepted approach for large-scale tumour sample analysis. In agreement with previous studies, gene amplification of CCND1 was associated with an overall adverse clinical outcome. The observed positive correlation between nuclear cyclin D1 expression and tumour grade and proliferation suggests a link between cyclin D1 and aggressive disease. In contrast, both higher nuclear expression and high cytoplasmic expression of cyclin D1 was instead associated with a decreased recurrence risk. Despite a positive association between cyclin D1 protein and CCND1 amplification status, both low nuclear fraction of cyclin D1 and CCND1 amplification were linked to earlier disease recurrence independently of other clinicopathological parameters - hence both factors serve as prognostic markers in endocrine-treated, ER-positive postmenopausal breast cancer. In patients not displaying CCND1 amplification, cytoplasmic expression of cyclin D1 was also an independent marker for longer TTR, indicating that the true prognostic value of cyclin D1 protein expression may be obscured by the CCND1-amplified cases: the clinicopathological significance of cyclin D1 expression might thus be best considered separately for amplified and nonamplified cases.
Apart from the role as a prognostic marker, cyclin D1 has been proposed as a predictive factor for tamoxifen response, as illustrated by poor clinical outcome in patients with ER-positive tumours with high cyclin D1 expression treated with tamoxifen [46]. These findings together with numerous experimental reports [33–35] support that cyclin D1 overexpression might abrogate the response to tamoxifen, as previously reported by our group and others [21, 46]. Our earlier discoveries have nevertheless been made in cohorts where patients were randomly assigned to receive either no adjuvant treatment or to receive tamoxifen [21, 36]. In the present study we compared the two endocrine therapies anastrozole and tamoxifen in relation to disease recurrence and cyclin D1 status, but there were no untreated control patients. There was no significant difference in treatment response between these two adjuvant therapies by stratification for cyclin D1 status, indicating that cyclin D1 is not a predictive marker for differences in response to anastrozole versus tamoxifen. No conclusions can be drawn, however, regarding cyclin D1 as a marker for general endocrine treatment resistance, since no untreated patients were available for analysis within the TransATAC study. Moreover, differences in tamoxifen response in relation to cyclin D1 in postmenopausal versus premenopausal breast cancer might exist. Our previous study reporting a potential unfavourable effect of tamoxifen included premenopausal patients exclusively, whereas this study focused exclusively on postmenopausal breast cancer cases and has shown no detrimental effect since the results for the two endocrine therapies were similar.
The relationship between cyclin D1, proliferation and prognosis is quite complex, with a positive correlation between cyclin D1 and Ki67 - Ki67 is associated with shorter TTR, while cyclin D1 is associated with longer TTR. Patients showing low expression of Ki67 had a longer TTR with the highest levels of cyclin D1 expression, whereas patients exhibiting higher levels of Ki67 had the shortest TTR with the lowest levels of cyclin D1 expression. Multivariate analysis identified the fraction cyclin D1-positive nuclei as a predictor of outcome independently of other clinicopathological parameters such as Ki67. These results suggest that, irrespective of proliferation status, intermediate to high expression of cyclin D1 results in a prolonged TTR in ER-positive, postmenopausal breast cancer patients. Similar results were observed in our previous study of randomised material from premenopausal breast cancer patients [47]. The relationship between cyclin D1, proliferation and prognosis hence seems to be complex, and this could in part be explained by potential additional functions for cyclin D1 unrelated to proliferation control, as well as co-amplification and co-deletion of specific genes on chromosome 11q13, the locus harbouring CCND1.
This study confirms that the cyclin D1 status provides independent prognostic information regarding ER-positive, postmenopausal breast cancers, supporting its emerging role as a biomarker that might be useful in the clinic. Our results demonstrate that high expression of cyclin D1 was associated with a reduced risk of recurrences, whereas amplification of the CCND1 gene was linked to an aggressive disease. Finally, no difference in response to anastrozole compared with tamoxifen was observed according to expression of cyclin D1 gene amplification or protein expression.
Arimidex, Tamoxifen, Alone or in Combination
confidence interval
chromogenic in situ hybridisation
oestrogen receptor
hazard ratio
tissue microarray
time to recurrence.
The authors thank Elise Nilsson for excellent technical implementation. This study was supported by grants from the Swedish Cancer Society, the Swedish Research Council, the Knut and Alice Wallenberg Foundation, Malmö University Hospital Research and Cancer Funds, Lund University Research Funds, South Swedish and South-East Swedish Breast Cancer groups and Breakthrough Breast Cancer Unit, Manchester, UK.
Authors' Affiliations
- Howell A, Dowsett M: Recent advances in endocrine therapy of breast cancer. BMJ. 1997, 315: 863-866. 10.1136/bmj.315.7112.863.View ArticlePubMedPubMed CentralGoogle Scholar
- Yoshida M, Haybittle JL, Davies C, Mason BH, Wilcken N, Ploner M, Yosef HMA, Lobelle JP, Peek U, Powell J, Mauriac L, Piccart MJ, Price JJ, Hupperets PSGJ, Ragaz J, Weiss RB, Abu-Zahra HT, Portnoj SM, Riley D, Gordon NH, Davis HL, Naja A, Romestaing P, Dubois JB, Mace-Lesec'h J, Rambert P, Barkmanova J, Owen JR, Meier P, Swindell R: Effects of chemotherapy and hormonal therapy for early breast cancer on recurrence and 15-year survival: an overview of the randomised trials. Lancet. 2005, 365: 1687-1717.View ArticleGoogle Scholar
- Brodie AM, Njar VC: Aromatase inhibitors in advanced breast cancer: mechanism of action and clinical implications. J Steroid Biochem Mol Biol. 1998, 66: 1-10. 10.1016/S0960-0760(98)00022-3.View ArticlePubMedGoogle Scholar
- Geisler J, King N, Dowsett M, Ottestad L, Lundgren S, Walton P, Kormeset PO, Lonning PE: Influence of anastrozole (Arimidex), a selective, non-steroidal aromatase inhibitor, on in vivo aromatisation and plasma oestrogen levels in postmenopausal women with breast cancer. Br J Cancer. 1996, 74: 1286-1291. 10.1038/bjc.1996.531.View ArticlePubMedPubMed CentralGoogle Scholar
- Baum M, Budzar AU, Cuzick J, Forbes J, Houghton JH, Klijn JG, Sahmoud T: Anastrozole alone or in combination with tamoxifen versus tamoxifen alone for adjuvant treatment of postmenopausal women with early breast cancer: first results of the ATAC randomised trial. Lancet. 2002, 359: 2131-2139.View ArticlePubMedGoogle Scholar
- Howell A, Cuzick J, Baum M, Buzdar A, Dowsett M, Forbes JF, Hoctin-Boes G, Houghton J, Locker GY, Tobias JS: Results of the ATAC (Arimidex, Tamoxifen, Alone or in Combination) trial after completion of 5 years' adjuvant treatment for breast cancer. Lancet. 2005, 365: 60-62.View ArticlePubMedGoogle Scholar
- Cuzick J, Sestak I, Baum M, Buzdar A, Howell A, Dowsett M, Forbes JF: Effect of anastrozole and tamoxifen as adjuvant treatment for early-stage breast cancer: 10-year analysis of the ATAC trial. Lancet Oncol. 2010, 11: 1135-1141. 10.1016/S1470-2045(10)70257-6.View ArticlePubMedGoogle Scholar
- Ormandy CJ, Musgrove EA, Hui R, Daly RJ, Sutherland RL: Cyclin D1, EMS1 and 11q13 amplification in breast cancer. Breast Cancer Res Treat. 2003, 78: 323-335. 10.1023/A:1023033708204.View ArticlePubMedGoogle Scholar
- Hui R, Ball JR, Macmillan RD, Kenny FS, Prall OW, Campbell DH, Cornish AL, McClelland RA, Daly RJ, Forbes JF, Musgrove EA, Robertson JF, Nicholson RI, Sutherland RL: EMS1 gene expression in primary breast cancer: relationship to cyclin D1 and oestrogen receptor expression and patient survival. Oncogene. 1998, 17: 1053-1059. 10.1038/sj.onc.1202023.View ArticlePubMedGoogle Scholar
- Brown LA, Johnson K, Leung S, Bismar TA, Benitez J, Foulkes WD, Huntsman DG: Co-amplification of CCND1 and EMSY is associated with an adverse outcome in ER-positive tamoxifen-treated breast cancers. Breast Cancer Res Treat. 2010, 121: 347-54. 10.1007/s10549-009-0479-x.View ArticlePubMedGoogle Scholar
- Sherr CJ, Roberts JM: CDK inhibitors: positive and negative regulators of G1-phase progression. Genes Dev. 1999, 13: 1501-1512. 10.1101/gad.13.12.1501.View ArticlePubMedGoogle Scholar
- Jiang W, Kahn SM, Zhou P, Zhang YJ, Cacace AM, Infante AS, Doi S, Santella RM, Weinstein IB: Overexpression of cyclin D1 in rat fibroblasts causes abnormalities in growth control, cell cycle progression and gene expression. Oncogene. 1993, 8: 3447-3457.PubMedGoogle Scholar
- Zhou P, Jiang W, Zhang YJ, Kahn SM, Schieren I, Santella RM, Weinstein IB: Antisense to cyclin D1 inhibits growth and reverses the transformed phenotype of human esophageal cancer cells. Oncogene. 1995, 11: 571-580.PubMedGoogle Scholar
- Wang TC, Cardiff RD, Zukerberg L, Lees E, Arnold A, Schmidt EV: Mammary hyperplasia and carcinoma in MMTV-cyclin D1 transgenic mice. Nature. 1994, 369: 669-671. 10.1038/369669a0.View ArticlePubMedGoogle Scholar
- Gillett C, Fantl V, Smith R, Fisher C, Bartek J, Dickson C, Barnes D, Peters G: Amplification and overexpression of cyclin D1 in breast cancer detected by immunohistochemical staining. Cancer Res. 1994, 54: 1812-1817.PubMedGoogle Scholar
- Bartkova J, Lukas J, Muller H, Lutzhoft D, Strauss M, Bartek J: Cyclin D1 protein expression and function in human breast cancer. Int J Cancer. 1994, 57: 353-361. 10.1002/ijc.2910570311.View ArticlePubMedGoogle Scholar
- McIntosh GG, Anderson JJ, Milton I, Steward M, Parr AH, Thomas MD, Henry JA, Angus B, Lennard TW, Horne CH: Determination of the prognostic value of cyclin D1 overexpression in breast cancer. Oncogene. 1995, 11: 885-891.PubMedGoogle Scholar
- Kenny FS, Hui R, Musgrove EA, Gee JM, Blamey RW, Nicholson RI, Sutherland RL, Robertson JF: Overexpression of cyclin D1 messenger RNA predicts for poor prognosis in estrogen receptor-positive breast cancer. Clin Cancer Res. 1999, 5: 2069-2076.PubMedGoogle Scholar
- Michalides R, Hageman P, van Tinteren H, Houben L, Wientjens E, Klompmaker R, Peterse J: A clinicopathological study on overexpression of cyclin D1 and of p53 in a series of 248 patients with operable breast cancer. Br J Cancer. 1996, 73: 728-734. 10.1038/bjc.1996.128.View ArticlePubMedPubMed CentralGoogle Scholar
- Gillett C, Smith P, Gregory W, Richards M, Millis R, Peters G, Barnes D: Cyclin D1 and prognosis in human breast cancer. Int J Cancer. 1996, 69: 92-99. 10.1002/(SICI)1097-0215(19960422)69:2<92::AID-IJC4>3.0.CO;2-Q.View ArticlePubMedGoogle Scholar
- Stendahl M, Kronblad A, Ryden L, Emdin S, Bengtsson NO, Landberg G: Cyclin D1 overexpression is a negative predictive factor for tamoxifen response in postmenopausal breast cancer patients. Br J Cancer. 2004, 90: 1942-1948. 10.1038/sj.bjc.6601831.View ArticlePubMedPubMed CentralGoogle Scholar
- Hwang TS, Han HS, Hong YC, Lee HJ, Paik NS: Prognostic value of combined analysis of cyclin D1 and estrogen receptor status in breast cancer patients. Pathol Int. 2003, 53: 74-80. 10.1046/j.1440-1827.2003.01441.x.View ArticlePubMedGoogle Scholar
- Naidu R, Wahab NA, Yadav MM, Kutty MK: Expression and amplification of cyclin D1 in primary breast carcinomas: relationship with histopathological types and clinico-pathological parameters. Oncol Rep. 2002, 9: 409-416.PubMedGoogle Scholar
- Dickson C, Fantl V, Gillett C, Brookes S, Bartek J, Smith R, Fisher C, Barnes D, Peters G: Amplification of chromosome band 11q13 and a role for cyclin D1 in human breast cancer. Cancer Lett. 1995, 90: 43-50. 10.1016/0304-3835(94)03676-A.View ArticlePubMedGoogle Scholar
- Buckley MF, Sweeney KJ, Hamilton JA, Sini RL, Manning DL, Nicholson RI, deFazio A, Watts CK, Musgrove EA, Sutherland RL: Expression and amplification of cyclin genes in human breast cancer. Oncogene. 1993, 8: 2127-2133.PubMedGoogle Scholar
- Bieche I, Olivi M, Nogues C, Vidaud M, Lidereau R: Prognostic value of CCND1 gene status in sporadic breast tumours, as determined by real-time quantitative PCR assays. Br J Cancer. 2002, 86: 580-586. 10.1038/sj.bjc.6600109.View ArticlePubMedPubMed CentralGoogle Scholar
- Clarke R, Leonessa F, Welch JN, Skaar TC: Cellular and molecular pharmacology of antiestrogen action and resistance. Pharmacol Rev. 2001, 53: 25-71.PubMedGoogle Scholar
- Zwart W, Rondaij M, Jalink K, Sharp ZD, Mancini MA, Neefjes J, Michalides R: Resistance to antiestrogen arzoxifene is mediated by overexpression of cyclin D1. Mol Endocrinol. 2009, 23: 1335-1345. 10.1210/me.2008-0268.View ArticlePubMedPubMed CentralGoogle Scholar
- Le Goff P, Montano MM, Schodin DJ, Katzenellenbogen BS: Phosphorylation of the human estrogen receptor. Identification of hormone-regulated sites and examination of their influence on transcriptional activity. J Biol Chem. 1994, 269: 4458-4466.PubMedGoogle Scholar
- Kato S, Endoh H, Masuhiro Y, Kitamoto T, Uchiyama S, Sasaki H, Masushige S, Gotoh Y, Nishida E, Kawashima H, Metzger D, Chambon P: Activation of the estrogen receptor through phosphorylation by mitogen-activated protein kinase. Science. 1995, 270: 1491-1494. 10.1126/science.270.5241.1491.View ArticlePubMedGoogle Scholar
- Pietras RJ, Arboleda J, Reese DM, Wongvipat N, Pegram MD, Ramos L, Gorman CM, Parker MG, Sliwkowski MX, Slamon DJ: HER-2 tyrosine kinase pathway targets estrogen receptor and promotes hormone-independent growth in human breast cancer cells. Oncogene. 1995, 10: 2435-2446.PubMedGoogle Scholar
- Holm C, Rayala S, Jirstrom K, Stal O, Kumar R, Landberg G: Association between Pak1 expression and subcellular localization and tamoxifen resistance in breast cancer patients. J Natl Cancer Inst. 2006, 98: 671-680. 10.1093/jnci/djj185.View ArticlePubMedGoogle Scholar
- Zwijsen RM, Wientjens E, Klompmaker R, van der Sman J, Bernards R, Michalides RJ: CDK-independent activation of estrogen receptor by cyclin D1. Cell. 1997, 88: 405-415. 10.1016/S0092-8674(00)81879-6.View ArticlePubMedGoogle Scholar
- Zwijsen RM, Buckle RS, Hijmans EM, Loomans CJ, Bernards R: Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998, 12: 3488-3498. 10.1101/gad.12.22.3488.View ArticlePubMedPubMed CentralGoogle Scholar
- Wilcken NR, Prall OW, Musgrove EA, Sutherland RL: Inducible overexpression of cyclin D1 in breast cancer cells reverses the growth-inhibitory effects of antiestrogens. Clin Cancer Res. 1997, 3: 849-854.PubMedGoogle Scholar
- Jirstrom K, Stendahl M, Ryden L, Kronblad A, Bendahl PO, Stal O, Landberg G: Adverse effect of adjuvant tamoxifen in premenopausal breast cancer with cyclin D1 gene amplification. Cancer Res. 2005, 65: 8009-8016.PubMedGoogle Scholar
- Dowsett M, Allred C, Knox J, Quinn E, Salter J, Wale C, Cuzick J, Houghton J, Williams N, Mallon E, Bishop H, Ellis I, Larsimont D, Sasano H, Carder P, Cussac AL, Knox F, Speirs V, Forbes J, Buzdar A: Relationship between quantitative estrogen and progesterone receptor expression and human epidermal growth factor receptor 2 (HER-2) status with recurrence in the Arimidex, Tamoxifen, Alone or in Combination trial. J Clin Oncol. 2008, 26: 1059-1065. 10.1200/JCO.2007.12.9437.View ArticlePubMedGoogle Scholar
- Harvey JM, Clark GM, Osborne CK, Allred DC: Estrogen receptor status by immunohistochemistry is superior to the ligand-binding assay for predicting response to adjuvant endocrine therapy in breast cancer. J Clin Oncol. 1999, 17: 1474-1481.PubMedGoogle Scholar
- McShane LM, Altman DG, Sauerbrei W, Taube SE, Gion M, Clark GM: Reporting recommendations for tumor marker prognostic studies (REMARK). J Natl Cancer Inst. 2005, 97: 1180-1184. 10.1093/jnci/dji237.View ArticlePubMedGoogle Scholar
- Arnould L, Denoux Y, MacGrogan G, Penault-Llorca F, Fiche M, Treilleux I, Mathieu MC, Vincent-Salomon A, Vilain MO, Couturier J: Agreement between chromogenic in situ hybridisation (CISH) and FISH in the determination of HER2 status in breast cancer. Br J Cancer. 2003, 88: 1587-1591. 10.1038/sj.bjc.6600943.View ArticlePubMedPubMed CentralGoogle Scholar
- Cuzick J: A Wilcoxon-type test for trend. Stat Med. 1985, 4: 87-90. 10.1002/sim.4780040112.View ArticlePubMedGoogle Scholar
- Dowsett M, Cuzick J, Wale C, Forbes J, Mallon EA, Salter J, Quinn E, Dunbier A, Baum M, Buzdar A, Howell A, Bugarini R, Baehner FL, Shak S: Prediction of risk of distant recurrence using the 21-gene recurrence score in node-negative and node-positive postmenopausal patients with breast cancer treated with anastrozole or tamoxifen: a TransATAC study. J Clin Oncol. 28: 1829-1834.
- Camp RL, Dolled-Filhart M, King BL, Rimm DL: Quantitative analysis of breast cancer tissue microarrays shows that both high and normal levels of HER2 expression are associated with poor outcome. Cancer Res. 2003, 63: 1445-1448.PubMedGoogle Scholar
- Weinstat-Saslow D, Merino MJ, Manrow RE, Lawrence JA, Bluth RF, Wittenbel KD, Simpson JF, Page DL, Steeg PS: Overexpression of cyclin D mRNA distinguishes invasive and in situ breast carcinomas from non-malignant lesions. Nat Med. 1995, 1: 1257-1260. 10.1038/nm1295-1257.View ArticlePubMedGoogle Scholar
- Zukerberg LR, Yang WI, Gadd M, Thor AD, Koerner FC, Schmidt EV, Arnold A: Cyclin D1 (PRAD1) protein expression in breast cancer: approximately one-third of infiltrating mammary carcinomas show overexpression of the cyclin D1 oncogene. Mod Pathol. 1995, 8: 560-567.PubMedGoogle Scholar
- Rudas M, Lehnert M, Huynh A, Jakesz R, Singer C, Lax S, Schippinger W, Dietze O, Greil R, Stiglbauer W, Kwasny W, Grill R, Stierer M, Gnant MF, Filipits M: Cyclin D1 expression in breast cancer patients receiving adjuvant tamoxifen-based therapy. Clin Cancer Res. 2008, 14: 1767-1774. 10.1158/1078-0432.CCR-07-4122.View ArticlePubMedGoogle Scholar
- Lehn S, Tobin NP, Berglund P, Nilsson K, Sims AH, Jirstrom K, Harkonen P, Lamb R, Landberg G: Down-regulation of the oncogene cyclin D1 increases migratory capacity in breast cancer and is linked to unfavorable prognostic features. Am J Pathol. 2010, 177: 2886-2897. 10.2353/ajpath.2010.100303.View ArticlePubMedPubMed CentralGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text:
STG & Rhythm game player
My Anime List - STG Scores - Twitter - My Figure Collection - osu! Profile
10,414 total episodes
Hey Sik, about ure avatar, is that what u look like or not? Just wondering that's all. }: )
Hah, you found me! ^^
Ah yeah indeed XD awesome!
Hey thanks a bunch, I have seen one of your top 5....onto skip beat! lol.


text:
We used to call them white goods, because that way everybody knew what we were talking about. Then we used to paint the cupboards around them in bright colours to introduce a bit of style to the kitchen. But if you want to be really fashionable this year, you need to paint the walls and cupboards white and use colour for the appliances.
Mark Scott of Steel Cuisine says: "People are buying kitchens in high-gloss white or black and introducing colour in the form of a brightly coloured cooker."
But you don't have to stop at the cooker; these days you can choose fridges and dishwashers in a rainbow of colours.
Britannia Dynasty cooker
The designer Nina Campbell has turned her attention to the humble cooker. Britannia's Dynasty range (from £4,599) has three patterns to choose from: Deauville, a silvery wave pattern; Damask, black flock designs; and Wisteria, pearlised flowers. All are versions of her fabrics and wallpapers, so you can co-ordinate the cooker with the curtains.
0800 073 1003; www.britannialiving.co.uk
Baumatic Dance Partners dishwasher
This range by Baumatic comes in red, orange or yellow and includes a cooker, washing machine and dishwasher. Prices start at £599 (for the dishwasher).
0118-9336 900; www.baumatic.co.uk
Ikea Framtid cooker & hood
If Ikea is following the trend, then the chances are that we all will be following it sooner or later. The Swedish retail giant's newest collection of kitchen designs and appliances includes a green combination oven and a matching cooker hood (prices are £495 and £599 respectively).
0845 355 1141; www.ikea.co.uk
Genesi range cookers
Choose from red, lime and dark green, although Scott, who starting selling the Genesi coloured ranges in February, says that so far yellow is proving most popular. However, for those people who aren't sure about the bright colours, he does offer a change-of-mind solution: "If someone panics about the colour at any point in the first two years, we will change it back again for £199," he says. So far, only two customers have used this service, although, he points out, two others have gone from stainless steel to colour. Prices start at £1,600.
01926 338 116; www.steelcuisine.co.uk
GE Fusion fridge-freezer
This classic American-style unit comes in 19 colours, although that includes two shades of white for the faint-hearted. Priced at £2,300.
01908 366 363; www.lsy-appliances.co.uk
Mini Om Cooker Hood
Too timid for a coloured cooker? Start with an apple green cooker hood, in glass. It's also available in turquoise, yellow or orange (from £1,598). Or try the Goldstream, which looks like a pendant lampshade (£1,999).
01252 351 111; www.elica.co.uk
- More about:
- Fireplaces And Ovens
- Glamour Magazine
- Kitchen (room)


text:
Results 1 to 6 of 6
- 12-19-2013, 08:50 AM Thread Author #1
- Posts
- 2 Posts
- Mentioned
- 0 Post(s)
- Tagged
- 0 Thread(s)
[APP][FREE][1.0][ANDROID] Football Quiz
Football Quiz - This is a brand new app that I have developed. I would appreciate your opinion.
This app features 8 levels of questions about football. Each level has a 26 questions that you have to answer. There is a limit percentage score that you should achieve in order to open the next level. Well, try your best and check how much do you know about football.
Here is the official description of the app in Google Playstore:
This is not just a game. This is passion! Passion fuelled by strong emotions, deserved wins, favourite teams and unforgettable football players. Yes, this is football! This is the game that makes you sit in front of your TV and forget the world around you. A Game, which incites passionate debates and prompts fan emotions.
This is the time proper for our Football Quiz application to become part of your football fervour. This is the one place you can prove your football experise. Football Quiz gives you the opportunity to learn and guess at facts pertaining to football teams, football stadiums, national and international football players, Bundesliga, Premier League, etc. The different levels in the game present a variable challenge. Every time you manage to successfully complete a level you automatically unlock an even greater one.
Here are some football topics you will find in our Football Quiz:
-Real Madrid
-Manchester United
-FIFA World Cup
-Premier League
-Top scorers through the years
Share this game with your friends, have fun and find out if they can match your knowledge about football.
Here is a link for Google Playstore, where you can download and test the app:
Here are some screenshots from the Football Quiz Game:
- 12-19-2013, 08:53 AM #2Banned
- Posts
- 7,910 Posts
- Global Posts
- 8,871 Global Posts
- Mentioned
- 0 Post(s)
- Tagged
- 0 Thread(s)
- Carrier
Re: [APP][FREE][1.0][ANDROID] Football Quiz
Very nice, thanks for sharing!
- 01-14-2016, 06:18 AM Thread Author #3
- 01-30-2016, 02:54 PM #5Junior Member
- Posts
- 0 Posts
- Mentioned
- 0 Post(s)
- Tagged
- 0 Thread(s)
Re: [APP][FREE][1.0][ANDROID] Football Quiz
I just tried it, definitely tests the knowledge you have about football.
Will show it to my friends and compare our scores.
One question though: Can you make switching to the next question go automatically?
I now have to press the arrow every time after I received the point(s), while I think an automatic transistion between questions will make the gameplay a bit easier.
A fun app nonetheless, I like it!
- Posts
- 7 Posts
- Global Posts
- 10 Global Posts
- Mentioned
- 0 Post(s)
- Tagged
- 0 Thread(s)
Re: [APP][FREE][1.0][ANDROID] Football Quiz
Oh wow, 100,000 downloads, Johnns777 can you share how you spread the word about your app so well? Did you do ad campaigns?
Similar Threads
[free][app] folk on the street !By dangerouspea in forum Tablet AppsReplies: 6Last Post: 09-05-2015, 06:46 AM
pixelated images on net and apps. help!By Twizel in forum Sprint Samsung Galaxy Note 3Replies: 1Last Post: 12-21-2013, 09:33 AM
Free Android App For Bullions and Base Metals TipsBy aryan jain1 in forum Android AppsReplies: 1Last Post: 12-19-2013, 03:29 PM
[FREE] Logo Quiz SagaBy HunkyDoryGames in forum Android AppsReplies: 1Last Post: 12-19-2013, 08:39 AM
Gmail App QuestionBy JAG21979 in forum Samsung Galaxy Note 3Replies: 2Last Post: 12-19-2013, 01:42 AM


text:
Tropical Fish Keeping - Aquarium fish care and resources (http://www.tropicalfishkeeping.com/forum.php)
- Freshwater Aquarium Equipment (http://www.tropicalfishkeeping.com/freshwater-aquarium-equipment/)
- - I want a good canister filter. (http://www.tropicalfishkeeping.com/freshwater-aquarium-equipment/i-want-good-canister-filter-19969/)
I want a good canister filter.
Hey, I got a lil X-mas $ so I decided I want a Canister filter for my 36 gallon. I look at them at the store and I dont know what a good one is. Basically I want something bullet proof, a simple well thought out design, cheap and easy to maintain, places to put carbon or whatever else I want, and minimal on the expensive proprietary crap. I am not into bells and whistles just somthin quality that does what it needs to. suggestions appreciated.
I would suggest eBay FuzzAz. I bought a Rena XP2 there less than a month ago for less than $100.
I use a fluval for my 46 gallon. They can be a bit pricey but the fluvals got little baskets for any filter media of your choice and you can customize ur filtering anyway you want. Its quite easy to maintain and its amazingly quiet.
keep an eye out on www.craigslist.com in your area, you can also post a wanted ad
|All times are GMT -5. The time now is 10:04 AM.
Powered by vBulletin® Version 3.8.8
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
vBulletin Security provided by vBSecurity v2.2.2 (Pro) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.
User Alert System provided by Advanced User Tagging (Pro) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.


text:
Emirates LNG Appoints Bank of Tokyo as Adviser, Mubadala SaysAnthony DiPaola
Emirates LNG, which plans to import liquefied natural gas into the United Arab Emirates, appointed Bank of Tokyo-Mitsubishi UFJ Ltd. as its financial adviser, according to one of the company's shareholders.
Mubadala Development Co. and International Petroleum Investment Co., two Abu Dhabi state-owned investment funds, are partners in the project to develop a receiving terminal at Fujairah on the U.A.E.'s eastern coast to import LNG, gas cooled to a liquid for transport by seaborne tanker.
Emirates LNG was formed as a project company to develop technical and financial plans for the facility and is consulting with Bank of Tokyo as its adviser, Kelly Thomson, Mubadala's head of structured finance, said at a conference in Dubai on Jan. 29. The company will further develop the scope of the project before settling on financing plans, she said.


text:
This Lent, take a 40-day devotional journey with Dietrich Bonhoeffer. Click here to sign up.
Sometimes God does not seem to answer prayer, causing his people to doubt him. Scripture affirms God's faithfulness to those who pray according to his will, and points out that the human understanding of a situation is limited.
Ex 5:22-23 See also Ge 15:2-3; Jos 7:7-9
Nu 11:11-15 See also 1Ki 19:4; Jer 15:15-18; Jer 20:7-9,14-18; Jnh 4:1-3; Mt 27:46 pp Mk 15:34; Ps 22:1
Job 10:2-22 See also Job 13:20-27; Job 14:1-22; Ps 13:1-2; Ps 42:9-10; Ps 44:22-26; Ps 77:7-9; Ps 80:4-6; Ps 88:6-9; Hab 1:2-3
God reiterates his promises to those who question him Ge 15:2-5 See also Ex 6:1-8
God provides help for those who question him 1Ki 19:1-8
God rebukes those who question him Job 40:1-9 See also Jer 15:19-21
God explains events to those who question him Hab 1:5-11
Starting your free trial of Bible Gateway Plus is easy. You're already logged in with your Bible Gateway account. The next step is to enter your payment information. Your credit card won't be charged until the trial period is over. You can cancel anytime during the trial period.
Click the button below to continue.
You've already claimed your free trial of Bible Gateway Plus. To subscribe at our regular subscription rate of $3.99/month, click the button below.
It looks like you're already subscribed to Bible Gateway Plus! To manage your subscription, visit your Bible Gateway account settings.
Try Bible Gateway Plus, a brand-new service that lets you experience Bible Gateway free of banner ads! It also gives you instant access to over 40 Bible study and inspirational devotional books, including the NIV Study Bible. With Bible Gateway Plus, you can experience and understand God's Word in life-changing new ways, without the distraction of ads. Try it free for 30 days—you can cancel at any time. Following your 30-day free trial, Bible Gateway Plus is only $3.99/month.
Three easy steps to start your free trial subscription to Bible Gateway Plus.


text:
The Bottle Rockets
Along with Uncle Tupelo, Missouri's Bottle Rockets have to be considered the godfathers of the '90s alt-country/roots-rock revival. Their sophomore effort, The Brooklyn Side, stands alongside Too Far to Care from the Old 97's as a landmark recording made during the movement's brief heyday. Since 1994, singer/guitarist Brian Henneman has led a variety of lineups through the course of being dumped by a major label and bouncing back with a competent batch of indie country-rock efforts. Pleasantly loud while playing tribute to Rust Never Sleeps, the Bottle Rockets, sadly, became just another great bar band.
Zoysia finds Henneman recharged by the economic and foreign policy failings of the Bush administration. Still mining those ever-sturdy Crazy Horse riffs, Henneman finds a way to incorporate soul and Southern funk into the roar while examining the personal toll W. and his ilk have taken upon the American middle class. "Better than Broken" and "Suffering Servant" are thoughtful examinations that never descend into rote, political rants by focusing on the day-to-day interactions of common people trying to make ends meet.
Still firmly grounded in greasy (and noisy), country shuffles, the Bottle Rockets have received a surprising rejuvenation by widening their margins. The playful call-and-response backing vocals on "I Quit" would have never appeared on previous efforts, but on Zoysia, such new wrinkles only add to the detailed characterizations--real folks taking solstice in beer and camaraderie--while cynically eyeing the powers that be.
The Bottle Rockets
The Bottle Rockets perform at the Granada Theater on Saturday, July 22, with the Gourds and the Weary Boys.
Get the Music Newsletter
Keep your thumb on the local music scene each week with music news, trends, artist interviews and concert listings. We'll also send you special ticket offers and music deals.
In Case You Missed It
Upcoming Events
Wade Bowen
TicketsFri., Mar. 3, 8:00pm
Green Day
TicketsSat., Mar. 4, 8:00pm
Loumuzik Live
TicketsSun., Mar. 5, 7:00pm
Led Zeppelin 2 -- A Tribute to Led Zeppelin
TicketsFri., Mar. 10, 7:00pmpowered by goldstar
Sponsor Content


text:
It might come as a surprise to most Americans that conservative political forces in Denmark strongly back climate regulation. As the ACES climate bill proved, attitudes toward climate legislation in the U.S. are still highly partisan -- the right arguing that it will somehow endanger the foundation of modern capitalism.
How is that conservatives in Denmark are so strongly supportive of the climate when their counterparts in the U.S. seem dedicated to thwart every attempt to move the climate debate forward?
Matthew Yglesias of the Center for American Progress posed this question to Connie Hedegaard, the Danish minister of Climate and Energy, and her response was enlightening:
1. In Denmark, 30 years of industry has developed around energy independence.
2. Preserving natural resources is considered a conservative value.


text:
Holiday Inn Express Southington Accommodation & Rooms
- Free buffet breakfast, free WiFi, and free parking
Main amenities
- 122 guestrooms
- Seasonal outdoor pool
- 24-hour fitness center
- 24-hour business center
- 24-hour front desk
- Coffee/tea in a common area
- Air conditioning
- Daily housekeeping
- Front desk safe
- ATM/banking services
- Laundry service
- Conference space
For families
- Free cribs/infant beds
- Rollaway/extra beds (surcharge)
- Microwave
- Refrigerator
- Connecting/adjoining rooms available
- Private bathroom
What's around
- Barnes Museum (2.2 mi / 3.5 km)
- Hawks Landing Country Club (2.6 mi / 4.2 km)
- Southington Public Library (2.6 mi / 4.2 km)
- Crescent Lake (3.1 mi / 4.9 km)
- Lake Compounce (4.2 mi / 6.8 km)
Holiday Inn Express Southington Accommodation & Rooms
Hotel Info: 0800 991 333from ZAR 1,182
- Room, 1 King Bed with Sofa bed, Non Smoking
- Room, 1 King Bed, Non Smoking
- Room, 2 Double Beds, Non Smoking
- Room, 1 Double Bed, Non Smoking
- Standard Room
- Room, 1 King Bed, Smoking
- One Double Bed Smoking
- Room, 2 Double Beds, Smoking
- One Double Bed Access Non Smoking
- Room, 1 Double Bed, Smoking
More great choices
At a glance
Key facts
Hotel size
- This hotel has 122 rooms
- This hotel is arranged over 3 floors
- Check-in time starts at 3 PM
- Check-out time is 11 AM
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- Pets not allowed
- Free WiFi in public areas
- Free WiFi and wired Internet access in rooms
- Free self parking
Nearby Two Star Southington hotels
In the hotel
- Free buffet breakfast
- Coffee/tea in a common area
- Outdoor seasonal pool
- 24-hour fitness facilities
- 24-hour business center
- Number of meeting rooms - 3
- Conference space
- Conference space size (feet) - 1200
- Conference space size (meters) - 108
- One meeting room
- 24-hour front desk
- Dry cleaning/laundry service
- Laundry facilities
- Free newspapers in lobby
- Multilingual staff
- Number of buildings/towers - 1
- ATM/banking
- Safe-deposit box at front desk
- Accessible bathroom
- In-room accessibility
- Roll-in shower
In the room
- Air conditioning
- Coffee/tea maker
- Iron/ironing board
- Private bathroom
- Hair dryer
- Pay movies
- Premium TV channels
- Desk
- Free WiFi
- Free local calls
- Refrigerator
- Microwave
- Daily housekeeping
- Connecting/adjoining rooms available
Top Southington hotels
Recently reviewed hotels in Southington
Nearby hotels
Recommended hotels
Holiday Inn Express Southington Accommodation & Rooms's small print
Also known as
- Holiday Inn Express Hotel Southington
- Holiday Inn Express Southington
- Holiday Inn Express Southington Hotel Southington
- Holiday Inn Southington
- Southington Holiday Inn
- Holiday Inn Express Southington Hotel
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.
Optional extras
Rollaway beds are available for USD 10 per night
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.
Recent reviews
Good 3.8 /5 from 121 reviews
Good location for business. Facility is older with a "paint" makeover. Bathrooms look like they are from the 90's. Broken floor tiles, dirty grout and trim. Overall no more than a 2 star motel with a "good" name.
Great stay as usual. Be back again
when I checked in, service was great. clerk was knowledgeable and funny. Wasn't stiff and awkward. we went upstairs to our room and quickly put our stuff down and headed out for the night. we got back around 130 am, and after a night of being the sober drive for an outing, all I wanted to do was Skype a relative in another country, but the Internet kept giving me an error code. I called guest service and asked if anything could be done, and he said, "no it works, I'm on it right now". didn't offer any help. I proceeded to tell him the error code, and he kept talking to me like I'm stupid. I work with computers every day. when something says ERROR CODE, it's an error code. you don't need to be a genius to figure that out. the error code was 1 digit off from the access code, and he kept repeating the access code like I was incompetent. after listening to him for 5 minutes, I hung up. so I forego the Internet and crawl into bed... where I'm greeted by tiny hairs all over the bed. not wanting to call the miserable human being at the front desk and bother him from his Internet surfing I slept in a chair. a f*****g chair. Wouldn't recommend unless you don't mind rude staff and unidentified cleanliness issues.
This hotel needs updated. Location is okay. Service was fine. Breakfast was awful - never saw anyone serve brown scrambled eggs! The rooms are old and dated and not very clean. This place needs revamped. The worst Holiday Inn Express I ever stayed at
The hotel is in need of new carpets throughout the common areas and the guest rooms. It generally needs a complete renovation. It is dingy and out of date compared to its competition.
Is the description of this hotel not correct? Tell us


text:
Shells with Peas and Mushrooms
This simple-but-satisfying pasta recipe is adapted from Lidia's Favorite Recipes, by Lidia Matticchio Bastianich and Tanya Bastianich Manuali, published by Alfred A. Knopf. Copyright 2012.
- Prep:
- Total Time:
- Servings: 6
Photography: Marcus Nilsson
Source: Everyday Food, November 2012
- Coarse salt and freshly ground pepper
- 2 tablespoons extra-virgin olive oil
- 2 slices bacon, finely chopped
- 1/2 cup chopped scallions
- 2 cups cleaned sliced mixed mushrooms, such as shiitake, oyster, and cremini
- 2 cups chicken stock or canned chicken broth
- 1 1/2 cups frozen baby peas, thawed
- 1 tablespoon unsalted butter
- 1 pound small pasta shells
- 1/2 cup freshly grated Grana Padano or Parmigiano-Reggiano (2 ounces)
Bring a large pot of salted water to a boil for pasta. In a large skillet, heat oil over medium. Add bacon and scallions and saute until scallions are wilted, about 4 minutes. Add mushrooms and sprinkle with salt. Continue to cook, stirring occasionally, until mushrooms have released their moisture, about 5 minutes.
Add stock, peas, and butter and season lightly with salt and pepper. Simmer until liquid is reduced by half, about 10 minutes. Check seasoning and adjust if necessary. Meanwhile, stir pasta into boiling water and cook, stirring occasionally, until al dente, about 12 minutes.
Drain pasta and return to pot. Add sauce and stir over low until pasta is coated. Remove from heat and add cheese. Toss well and serve.


text:
Inside Dynaric
For over 40 years, Dynaric has built a solid reputation as a vital and innovative leader in the plastic strapping market with products that have increased efficiency and productivity, while lowering overall costs.
Dynaric operates one of the largest plastic strapping manufacturing facilities in the United States, located in Virginia Beach, VA. There we manufacture a complete line of plastic strapping products including machine grade, controlled elongation and high tensile. Dynaric is a leading supplier of plastic strapping machines and custom systems including basic semi-automatic table top machines, through high speed arch type units, culminating with fully automatic, high speed, operator- less equipment and complete systems.
See below for a list of upcoming tradeshows that Dynaric will be attending.
- Find Solutions for Your Packaging Needs at 2017 ProMat
Chicago, IL
April 3-6, 2017
- America East 2017 offers a powerful lineup for anyone working in the news media industry!
Hershey, PA
April 10-12, 2017
Dynaric in the news.
See what's happening at Dynaric.
- Dynaric, Inc., a leading manufacturer of plastic strapping and strapping ma...
- Dynaric, Inc. has introduced AM650, an economical high speed automatic box...


text:
Full profile →'">
The author is a Forbes contributor. The opinions expressed are those of the writer.
Loading ...
Loading ...
This story appears in the {{article.article.magazine.pretty_date}} issue of {{article.article.magazine.pubName}}. Subscribe
In early trading on Monday, shares of Microsoft (MSFT) topped the list of the day's best performing Dow Jones Industrial Average components, trading up 1.6%. Year to date, Microsoft registers a 9.5% gain.
And the worst performing Dow component thus far on the day is Coca-Cola (KO), trading down 0.1%. Coca-Cola is lower by about 5.8% looking at the year to date performance.
Two other components making moves today are Verizon Communications (VZ), trading up 0.3%, and Walt Disney (DIS), trading up 1.4% on the day.
Page {{article.article.page + 1}} / {{article.article.pages.length}} Continue