text:
Relationship between anti-dsDNA, anti-nucleosome and anti-alpha-actinin antibodies and markers of renal disease in patients with lupus nephritis: a prospective longitudinal study
DOI: 10.1186/ar2831
© Manson et al.; licensee BioMed Central Ltd. 2009
Received: 12 August 2009
Accepted: 14 October 2009
Published: 14 October 2009
Glomerulonephritis is a major cause of morbidity and mortality in patients with systemic lupus erythematosus (SLE). Deposition of autoantibodies in the glomeruli plays a key role in the development of lupus nephritis (LN). Different groups have proposed that either anti-nucleosome antibodies or antibodies that bind the intrinsic renal antigen, α-actinin, are central to the pathogenesis of LN. These theories have been based mainly on cross-sectional studies in patients and on experiments in animal models. No previous longitudinal studies have compared the relationships between levels of these antibodies and markers of renal function. We assessed how well anti-α-actinin, anti-nucleosome and anti-double-stranded DNA (anti-dsDNA) antibodies reflected renal outcome measures in patients with new-onset LN followed for up to 2 years.
Renal disease activity was monitored by measuring urine protein/creatinine ratio (PCR), serum albumin and a composite outcome of renal remission. At each time point, anti-nucleosome and anti-α-actinin antibodies were measured by enzyme-linked immunosorbent assay. High-avidity anti-dsDNA antibodies were measured using the Farrzyme assay. We analysed relationships between levels of the three antibodies and between antibody levels and renal outcome measures over time.
Levels of anti-nucleosome and anti-dsDNA were positively correlated with each other (r = 0.6, P = 0.0001) but neither correlated with anti-α-actinin level. At baseline, mean anti-nucleosome levels were higher in patients with LN than in healthy controls (0.32 versus 0.01, P < 0.001). The same was true for anti-dsDNA antibodies (0.50 versus 0.07, P < 0.001) but not for anti-α-actinin (0.33 versus 0.29). Over the follow-up period, anti-nucleosome and anti-dsDNA levels associated positively with urine PCR (P = 0.041 and 0.051, respectively) and negatively with serum albumin (P = 0.027 and 0.032, respectively). Both anti-nucleosome and anti-dsDNA levels were significantly lower during renal remission than when renal disease was active (P = 0.002 and 0.003, respectively). However, there was no relationship between anti-α-actinin levels and urine PCR, serum albumin or remission status.
This prospective longitudinal clinical study is the first to compare levels of anti-nucleosome, anti-dsDNA and anti-α-actinin antibodies in the same patients with SLE. Our results support the concept that, in the majority of patients, anti-nucleosome antibodies play a major role in pathogenesis of LN, in contrast to anti-α-actinin antibodies.
Lupus nephritis (LN) occurs in 40% to 60% of patients with systemic lupus erythematosus (SLE) [1]. Koffler and colleagues [2] first demonstrated deposition of autoantibodies in LN renal tissue. A range of evidence from clinical [3], renal biopsy [4] and animal [5–7] studies suggested that anti-double-stranded DNA (anti-dsDNA) antibodies were the main autoantibodies involved in the pathogenesis of LN. It has been argued that high-avidity anti-dsDNA antibodies are particularly linked to pathogenicity, and some laboratory tests have been developed specifically to test for these high-avidity antibodies [8]. However, there are clearly some patients with persistently high anti-dsDNA levels who never develop LN [9] and there is no simple relationship between the ability of passively transferred monoclonal antibodies to bind dsDNA and the ability of the same antibodies to cause glomerulonephritis [5–7].
In some cases, modification of antibodies by mutagenesis increased binding to dsDNA but reduced pathogenicity [7]. In other cases, pathogenic monoclonal antibodies were found not to bind dsDNA at all after rigorous purification and were actually anti-nucleosome antibodies [10, 11]. Furthermore, when a rat kidney perfusion system was used, glomerular binding of monoclonal antibodies was shown to require the presence of nucleosomes [12]. It has therefore been argued that binding to nucleosomes is a major determinant of pathogenicity of autoantibodies in LN [13, 14].
An alternative theory holds that direct cross-reaction of anti-dsDNA with intraglomerular antigens is key [13, 15]. Although cross-reactivity with a number of proteins (including laminin and type IV collagen) has been postulated (reviewed in [13]), the importance of anti-α-actinin antibodies has been particularly stressed in recent years. This emphasis on the possible pathogenic role of anti-α-actinin antibodies has arisen as a result of studies in murine models [6, 16] and clinical studies [17–19], although anti-α-actinin antibodies could not be eluted from glomerular deposits in mice with LN [20]. However, no previous study has compared anti-nucleosome and anti-α-actinin antibody levels in the same patients.
In this study, we identified 16 patients with new-onset LN and followed them prospectively for up to 2 years. We tested their blood for both anti-nucleosome and anti-α-actinin antibodies, allowing (for the first time) direct comparison of both of these important specificities in the same patients with LN. Furthermore, we examined the associations between levels of both anti-nucleosome and anti-α-actinin antibodies, levels of high-avidity anti-dsDNA antibodies and markers of renal disease, including an assessment of whether the patients entered renal remission.
Materials and methods
Sixteen patients with new-onset biopsy-proven LN were recruited prospectively from the Lupus Clinics at University College London and St Thomas' hospitals (London). All of the patients fulfilled the American College of Rheumatology revised criteria for SLE [21, 22]. Blood samples were taken at the time of recruitment and then at routine follow-up appointments and were spun at 500 g for 10 minutes to produce serum, which was stored at -20°C. To establish normal values for the enzyme-linked immunosorbent assays (ELISAs) used, 30 healthy volunteer donors with age and gender distributions similar to those of the controls were recruited from staff at University College London Hospital and University College London. Serum was collected and stored as above. Patients and healthy controls gave informed consent. This study received approval from the Thames Valley Multi-Centre Research Ethics Committee (reference number 04/MRE12/58) and was passed by the Joint University College London/University College London Hospitals Committee on the Ethics of Human Research and the St Thomas' Hospital Research Ethics Committee.
Renal outcome measures
For each patient at each time point, urine was tested for protein/creatinine ratio (PCR), and serum was tested for albumin and creatinine in the routine clinical laboratory. Statistical analysis was carried out on three outcome measures: absolute values of urine PCR and serum albumin and a composite score for renal disease activity. This composite score was defined using measurements of urine PCR and serum albumin and creatinine. Complete renal remission was defined as follows: PCR of not more than 30 mg/mmol, normal serum albumin and normal serum creatinine. Partial remission was defined as follows: decrease in urine PCR by at least 50%, serum albumin of at least 30 g/L, and either normal serum creatinine if the baseline creatinine was less than 260 μmol/L or a 50% decrease in creatinine if the baseline value was at least 260 μmol/L. Patients who did not fulfil these criteria for either complete or partial remission were considered to have active LN.
Human IgG anti-nucleosome enzyme-linked immunosorbent assay
Serum samples were diluted 1:800 in phosphate-buffered saline/0.05% Tween 20 (PBS-T) and tested in duplicate for binding to nucleosomes prepared from Jurkat cells. The methods for obtaining nucleosomes and carrying out the ELISA have been described previously [9, 23]. Monoclonal human IgG antibodies with well-defined anti-nucleosome-binding properties [23] were used as positive and negative controls. To standardise results between ELISA plates, readings were taken when the positive control had reached an optical density (OD) of approximately 1.2.
Human IgG anti-α-actinin enzyme-linked immunosorbent assay
Serum samples were diluted 1:100 in PBS-T and tested in an anti-α-actinin ELISA. The ELISA method was as described previously [17]. Again, the positive and negative controls were human monoclonal antibodies produced in our laboratory with known anti-α-actinin-binding properties [23]. Plates were read when the OD of the positive control reached 1.2.
Detection of high-avidity human IgG anti-double-stranded DNA antibodies
High-avidity anti-dsDNA antibody titre was measured using the Farrzyme assay (The Binding Site, Birmingham, UK) in accordance with the instructions of the manufacturer [8].
Expression of results of enzyme-linked immunosorbent assay tests
ODs from all of the assays were converted to absorbance ratios (ARs) to standardise the data and minimise interassay variation. The mean OD for each sample was calculated from the duplicates. The mean OD was then divided by the standard positive control on that assay plate to give the AR.
Statistical analysis
The data were analysed in GraphPad Prism (GraphPad Software Inc., San Diego, CA, USA) and using the 'xt' commands for longitudinal data in Stata 9.2 (StataCorp LP, College Station, TX, USA). The aim of the analysis was to assess which antibody level (high-avidity anti-dsDNA, anti-nucleosome or anti-α-actinin) best reflected the renal outcome measures, PCR, albumin and remission status. First, the continuous laboratory variables (all three antibodies, albumin and PCR) were tested for normality and, if necessary, were transformed using log transformation. PCR measurements and ARs for anti-nucleosome and high-avidity anti-dsDNA antibodies were so transformed, and consequently changes in these measurements are presented as percentages rather than as absolute values. Mean ARs for the baseline binding data in the patient and control groups were compared using the Student t test using the Satterthwaite approximation for unequal variances where appropriate. Possible correlations between the results of the three antibody assays were investigated by calculating the Pearson correlation coefficient, using log-transformed data where appropriate. For the two continuous outcome variables (albumin and PCR), each explanatory variable was analysed one at a time and significant variables then were included in a multivariable sensitivity analysis. Maximum likelihood random effects models were used to fit linear regression models with random intercepts. The residuals from all models were checked for normality using a normal plot. The relationship between remission status and the three laboratory explanatory variables was investigated using the regression specification for a one-way analysis of variance. Anti-nucleosome level, anti-α-actinin level and high-avidity anti-dsDNA level were each considered in turn as the outcome with remission as the explanatory variable. The Wald test was used to test contrasts between the coefficients for the different levels of renal remission (active disease, partial remission and complete remission). Due to the size of the dataset, there was insufficient statistical power to carry out multivariable analysis, and significant results from these analyses should be treated with caution.
Characteristics of patients and control subjects
Baseline patient data
Patient ID
Biopsy result
Urine PCR, mg/mmol
Serum albumin, g/L
Treatment, daily dose in mg
P (20), MPa
P (5), Ca
P (60), Ca
P (20), M (1,000), Ca
H (400), MPa
Changes in antibody levels over the follow-up period
Follow-up dates were determined by the need of the patient to attend the clinic. The mean number of weeks over which the patients were followed was 37.3 (range 10 to 85). The mean interval between test points was 9.3 weeks (range 2 to 26). All patients had samples collected on at least three occasions, with a median number of time points per patient of 4.5 (range 3 to 8). On average, all three assays demonstrated significant downward linear trends over time. Anti-nucleosome antibodies decreased by 1.6% per week, and high-avidity anti-dsDNA antibodies decreased by 0.8% per week. Anti-α-actinin antibodies decreased by 0.0015 per week, which (given the mean baseline AR for anti-α-actinin antibodies of 0.25) is the equivalent of a 0.6% decrease per week. In general, if anti-nucleosome antibodies were high for any patient at any time point, so were high-avidity anti-dsDNA antibodies. By contrast, high anti-α-actinin antibodies (taken as an AR of at least 0.5) were detected in only three patients (LN3, LN9 and LN15) and were seen in combination with low AR for the other two assays. Six patients had low titres (AR <0.25) of anti-nucleosome and high-avidity anti-dsDNA antibodies throughout the study period.
There were significant negative linear trends between albumin levels and anti-nucleosome (P = 0.027) and high-avidity anti-dsDNA (P = 0.032) antibody titre. Repeating the analysis after exclusion of patient LN11 confirmed the analysis and showed stronger levels of statistical significance (P = 0.001 and 0.011, respectively). On average, albumin decreased by 1.16 g/L for every twofold increase in anti-nucleosome titre and by 1.59 g/L for every twofold increase in high-avidity anti-dsDNA antibody titre. There was no relationship between anti-α-actinin levels and either urine PCR (P = 0.401) or serum albumin (P = 0.332).
Remission status
Analysis of the three-level remission score (active disease, partial remission and complete remission) demonstrated significant differences between the groups in all three antibody levels (anti-nucleosome, P = 0.001; high-avidity anti-dsDNA, P = 0.0063; and anti-α-actinin, P = 0.0368). Application of the Wald test (which assesses whether there is a true difference between groups) suggested that there was no real difference in the levels between partial and complete remission. The partial and complete remission categories were combined such that there were only two possible renal activity levels for each patient at each time point (active or remission), and the data were then re-analysed. The association between activity status and anti-nucleosome or high-avidity anti-dsDNA antibody titre was maintained (P = 0.002 and 0.003, respectively), but there was no observed difference in the levels of anti-α-actinin antibodies between active disease and remission. On average, anti-nucleosome levels were 53.8% lower and high-avidity anti-dsDNA levels were 34.5% lower when patients were in remission than when they had active disease.
Longitudinal studies in individual patients
This is the first clinical study to achieve a direct comparison of the associations between anti-nucleosome, high-avidity anti-dsDNA and anti-α-actinin levels and outcomes of renal disease in patients with LN followed longitudinally. The results show that assessment of any renal outcome measure (whether serum albumin, urine PCR or remission) over time favoured the theory that renal disease activity was linked to the presence of anti-nucleosome and anti-dsDNA antibodies and not to anti-α-actinin antibodies.
Nucleosomes released in apoptotic debris, but not cleared efficiently from the circulation [25], are critical immunogenic stimulants for both T cells and B cells in SLE [13, 26]. The mechanism by which anti-nucleosome antibodies bind glomeruli has been reviewed extensively elsewhere [13, 14]. Recent electron microscopy data from renal biopsies of both human and murine LN confirm that autoantibodies in those tissues co-localise with electron-dense extracellular deposits of chromatin [27, 28]. High titres of anti-nucleosome antibodies have been found in up to 87% of patients with SLE [26, 29, 30], and some studies have noted a particular association with renal disease [31, 32]. When highly purified nucleosomes are used as the antigen, the anti-nucleosome assay is very specific for patients with SLE [33]. However, these serological studies either were cross-sectional or did not evaluate quantitative markers of renal impairment [30].
A Dutch study of 52 patients with proliferative LN [34], followed longitudinally for a year as part of a clinical trial, showed good correlation between these two serological measures (r = 0.63, P < 0.001), as in our study. Data on renal outcomes across the whole patient group were reported in terms of relapse or remission, but data on serum albumin and urine PCR were not given. The investigators did not observe increases in anti-nucleosome or anti-dsDNA antibody titre prior to renal relapse. There was no association between levels of anti-nucleosome antibodies at disease entry and occurrence of relapse or time to remission. Data on individual patients were not given.
Alpha-actinin-4 is an actin-binding protein present in both podocytes and mesangial cells. Two groups showed that the ability of murine monoclonal anti-dsDNA or anti-nucleosome antibodies to cause pathogenicity in mice was related to their ability to cross-react with α-actinin [6, 16], and one of the groups then showed that a cross-reactive human anti-dsDNA/anti-α-actinin antibody caused glomerulonephritis in these mice [35]. However, the electron-dense deposits that are the sites of autoantibody deposition in lupus-prone NZB/W F1 mice do not co-localise with α-actinin. Three previous clinical studies have looked at anti-α-actinin levels in patients with SLE [17–19]. One study showed that purified anti-dsDNA antibodies from patients with SLE were more likely to cross-react with α-actinin if the patients had nephritis [17]. Two subsequent studies [18, 19] were both cross-sectional. Both groups showed that anti-α-actinin antibodies can occur in patients with diseases other than SLE (for example, rheumatoid arthritis [19] and autoimmune hepatitis [15]) and occur in both patients with LN and patients with lupus but not nephritis. In one study, positivity for anti-α-actinin distinguished patients with nephritis from those without nephritis more clearly than positivity for anti-dsDNA, although only 10 out of 24 patients with nephritis were positive for anti-α-actinin [18]. Neither study looked at anti-nucleosome antibody levels, and neither showed any correlation between levels of anti-α-actinin antibodies and indicators of renal disease such as proteinuria. To our knowledge, ours is the first longitudinal study of anti-α-actinin levels in patients with LN. Although our results do not favour an important role for cross-reactive anti-α-actinin antibodies in most patients with LN, they leave open the possibility that these antibodies may be important in a minority of such patients. Three of our 16 patients had increased levels of anti-α-actinin antibodies but not anti-dsDNA or anti-nucleosome antibodies. This is in contrast to the study of Renaudineau and colleagues [18], who found that 21 out of 22 patients with SLE who had increased anti-α-actinin levels also had increased anti-dsDNA. Fewer than half of these 22 patients had LN.
Although our results are important in shedding more light on the pathogenesis of LN, they do not suggest any changes in clinical practice. Neither anti-nucleosome nor anti-α-actinin tests seem likely to be better predictors of renal outcome than anti-dsDNA ELISA, which is already widely used for monitoring patients with SLE. Improved monitoring of renal disease in patients with LN is more likely to be achieved by combining anti-dsDNA tests with assays more specific for renal dysfunction, such as urinary gelatinase B-associated lipocalin (nGAL), which has been shown to be a marker of renal disease in cross-sectional studies of both adult [36] and paediatric [37] SLE.
This is the first prospective longitudinal study of patients with new-onset biopsy-proven LN to study antibody levels and renal outcome measures at multiple time points within the first two years after diagnosis. In particular, we measured both anti-nucleosome and anti-α-actinin levels, specificities that have been studied previously in separate groups of patients with LN but never in the same group. The most important conclusion of our study is that anti-nucleosome and high-avidity anti-dsDNA antibodies are much more closely related to renal outcome measures in the majority of these patients than anti-α-actinin levels.
- anti-dsDNA:
anti-double-stranded DNA
absorbance ratio
- dsDNA:
double-stranded DNA
enzyme-linked immunosorbent assay
lupus nephritis
optical density
phosphate-buffered saline/0.05% Tween 20
protein/creatinine ratio
standard deviation
systemic lupus erythematosus.
JJM and LJM were supported by the Arthritis Research Campaign (grants 16555 and 17045, respectively). Farrzyme kits were donated by The Binding Site (Birmingham, UK). This work was undertaken at University College London Hospital/University College London, which received a proportion of funding from the funding scheme of the National Institute for Health Research Biomedical Research Centres of the Department of Health.
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Reviewed By Keith M (Visalia, CA) Reviewed for a 2011 GMC Acadia —9/14/2016 8:20 AM
I can't tell you how easy this was to install. 15 minutes tops and I was looking for a self tapping sheet metal screw. Fit perfect and plugged in fine.
Reviewed By Wilbur T (GREAT FALLS, MT) Reviewed for a 1978 Ford F-250 —11/14/2016 9:50 AM
My truck already has a 4-way flat, so this made the installation easier and cleaner. Splicing into wires is a necessary evil at times and this avoids that unpleasantness. The bracket needed a small bend to allow the door to open all the way so the plug-in will fit. This is the one with the LED lights that indicate which pins are working, but I wish I had purchased a separate tester instead. One LED light always stays on. Battery drain isn't a concern, but unnecessarily attracting attention is.

subset: commoncrawl


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by Duncan Larkin, Published Jun. 15, 2012, Updated Jun. 15, 2012 at 11:01 AM UTC
He has a chronic foot injury.
by Duncan Larkin, Published Aug. 29, 2011, Updated Aug. 30, 2011 at 5:29 AM UTC
She hadn't been able to run for four months this year.
by Duncan Larkin, Published Jul. 28, 2011, Updated Nov. 21, 2016 at 1:57 PM UTC
Her father, a doctor, thinks it's plantar fasciitis.
by Duncan Larkin, Published Jul. 7, 2011, Updated Jul. 7, 2011 at 10:52 AM UTC
She had recently defeated Paula Radcliffe in the Bupa London 10,000m.
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More Videos...
Rebuilding a QA1 Circle Track Twin Tube Shock - QA1 Tech
www.qa1.net 800.721.7761 Learn to rebuild a QA1 twin tube shock in just minutes for cleaning or replacing parts. Watch Marshall take apart and put back together a shock absorber that is rebuildable and revalveable.
Penske Racing Shocks SEMA 2012
This is our SEMA 2012 video. Meet some of our dedicated engineers and technicians as they explain our industry leading racing damper technology.
I don't think I have and Nitrogen in my Shock
Here is an explanation of what to look for if you don't think you have any nitrogen left in your shock Check us out at www.maultechatv.com
Vacuum Pump by Lainer Suspension racing
The best Lainer Suspension's product! Made in Italy by Lainer Suspension Racing: the designer and the builder!! This machine allowed to speed up enormously purging air inside the shock absorbers, after have cleaned and assembled all parts of shock absorbers. • Transparent double tube from bladder to Pump, without need to open compression adjustment!! • Speed of purge 4/5 minutes only!! • Many possibility of use, in base of work habits!! • Is Possible to use the Pump on the track for test session, using the same oil inside shock, only changing mode to purge. • Very small dimension (580mmx344mmx194mm), you can take with you the pump on track by comfortable handles!! • The pump is build with aluminium and stainless steel, specials fittings for vacuum, and completely Made in Italy!! • Acoustic Alert for processing ending and secure valves! It's delivered with all accessories for all shock absorbers, After Market or standard.


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The Miami Rail Fundraiser Promises Booze, BBQ, and a Bounce House
Spiked sno cones. Tasty barbeque. A bounce house and a dunk tank. The grown-up carnival of your dreams is coming to Miami, and it's all due to the Miami Rail.
With the release of its upcoming issue, Miami's own literary journal and sister publication to the Brooklyn Rail is hosting an evening of delightful activities to celebrate its first successful year. But though the Rail is known for its intellectual and critical coverage of Miami culture, this fundraiser isn't some stodgy arts soiree.
Nina Johnson-Milewski, founder and publisher for the Rail, categorizes the event as "fun" themed. She says that aside from raising money to facilitate publication of future issues, "we're excited to gather all of our supporters in one place and build bridges to new potential readers who will join us for a party and hopefully leave with a subscription." Previous issues will also be for sale at the "fun-raiser."
Also on offer: a BBQ, tequila spiked snow cones, and vodka ice pops for the grownups, and for the enjoyment of the young at heart, a bounce house, three-legged race, and dunk tank.
Upcoming Events
The Spotlight
TicketsFri., Feb. 24, 10:00pm
After Hours
TicketsFri., Feb. 24, 11:00pm
Jeff Laub... Laughing Out Laub
TicketsSat., Feb. 25, 8:00pm
Just the Funny Mainstage Show
TicketsSat., Feb. 25, 9:00pm
A Ukrainian Montage
TicketsSun., Feb. 26, 2:00pm
Next to her role as publisher for the Rail, Johnson-Milewski is also owner of Gallery Diet in Wynwood. Though she says how both roles are independent of each other and she has a wonderful team to assist her in both worlds, there's no denying she is significantly contributing to elevating Miami's cultural voice.
"The Rail is all about building a document of our time and place via intelligent print dialog... it's writing that comes from an inside and in-depth perspective on the arts," she says. Gallery Diet, on the other hand, "is about creating a two-way window that shows Miami to the international art community, but that also brings what's globally relevant into our own backyard."
For the fundraiser, Johnson-Milewski has a list of hosts comprised of others who are also shakers in the art world. "You think I play many roles? Our fun-raiser hosts put me to shame," she jokes. By day, these hosts are museum directors, curators, business leaders, marketing professionals, lawyers, and of course writers. And by night, they work to raise awareness of the Rail and sell tickets for the event. "We may also be getting some of them in the dunk tank!" says Johnson-Milewski.
"Our first year was truly exciting and I'm proud of the fact that with every new issue we always say 'This is our best and biggest issue yet.' That's a goal we continue to aim for as we begin our second year; we're also looking to expand our public programs and institutional partnerships."
The fun-raiser will take place in the home of Johnson-Milewski (986 NE 84th St., Miami) on Sunday, October 6, beginning at 5 until 9 p.m. Tickets may be purchased online at miamifoundation.org/givenow or by emailing firstname.lastname@example.com; prices are $75 for one person or $125 for a couple.
Follow Cultist on Facebook and Twitter @CultistMiami.
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In Case You Missed It
Upcoming Events
The Doo Wop Project
TicketsFri., Feb. 24, 8:00pm
Jerry Seinfeld
TicketsFri., Feb. 24, 8:00pm
JTF's Friday Night Live
TicketsFri., Feb. 24, 9:00pm
Jackie Mason Dinner Show
TicketsTue., Feb. 21, 7:30pmpowered by goldstar
Sponsor Content


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File Directory Picker Java Applet or Flash
This project received 2 bids from talented freelancers with an average bid price of $100 USD.Get free quotes for a project like this
Project BudgetN/A
Total Bids2
Project Description
We need a downloadable Java applet that allows a user to select a directory and provide the directory path to a server-side script written in .NET.
1) An Java applet that can be instantly downloaded and run within a specific area on a Web browser. (Again, the applet must run within the browser).
2) Shows the PC's file directory hierarchy, and allow the user to navigate to the desired directory and select it.
3) Once a user select a directory, automatically upload up to 10 files within that directory to the Web server, which receives the files via a server-side script (preferrable written in ASP/[url removed, login to view]) and saves the files to a server directory (customizable in the script).
4) Send the selected directory name and path via HTTP Post to the Web server (which can be received by any server-side script)
Additional requirements:
1) The Java applet needs to support all the popular browsers, including IE, Fire Fox, Opera, Safari, etc. on multiple platforms.
2) The Java applet needs to show the progress of the file upload, and display an error message if the upload fails or if it is unable to send the directory name and path to the Web server.
3) The server-side script must support IIS 5.0 and 6.0. Preferrabley it is written in ASP or [url removed, login to view], but we may accept PHP or Java Script.
4) The Java applet can be signed with a valid digital certificate. For the time being it can be signed by any certificate.
5) If someone has a way to do this using Flash to meet all the requirements, we will also consider to accept the solution.
Work will start immediately.
Caution: The applet is not to select files to upload (we know how this can be done with HTML). It is to pick a directory and send the directory name to the server, in addition to automatically uploading the files. The user doesn't get to select the files to upload.
Skills Required
Looking to make some money?
- Set your budget and the timeframe
- Outline your proposal
- Get paid for your work
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Work on projects like this and make money from home!Sign Up Now
- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online


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About Us
The Salvation Army, an international movement, is an evangelical part of the universal Christian Church. Its message is based on the Bible. Its ministry is motivated by the love of God. Its mission is to preach the gospel of Jesus Christ and to meet human needs in His name without discrimination.
Area Commanders
Majors Pete & Cathy Costas
Army Structure
Early on in its history, The Salvation Army was set up with a quasi-military structure patterned after the ranks of the British Armed Forces. This structure has contributed to a reputation for discipline and mobility that serves the organization well. The Salvation Army is actually made up of several sub-groups, equally important, and instrumental in accomplishing the mission of spreading the gospel and serving humanity.
Commissioned from one of four Colleges for Officer's Training in the United States, officers are full-time personnel with responsibility for leadership of both the spiritual and social services work of The Salvation Army. Their ranks descend from General (the international leader stationed at International Headquarters in London) down to captain (the rank assigned upon commissioning).
Soldiers are members who maintain their congregational church home at The Salvation Army and have signed a statement of beliefs, pledging to live up to a high code of moral conduct. They usually are employed outside of The Salvation Army in all manner of vocations and careers.
Advisory Board Members
Advisory Board members supply the backbone of public support that sustains The Salvation Army in a local community. Normally comprised of influential business leaders and public officials, this board of volunteers advises The Salvation Army on matters of community priorities and needs. They also serve as ambassadors among the influential-enlisting financial support for the cause. Advisory Councils serve as special program arms of the Advisory Board and are in place for The Salvation Army Boys & Girls Club, Pickens County, and Oconee County.
For all media inquiries, please contact Marketing and Special Events Coordinator, Chelsia Spivey at firstname.lastname@example.com or (864)235-4803 ext. 224


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First, apologies for a long pause between postings. I was in the middle of changing jobs -- I'm now at EVault, a Seagate subsidiary that offers cloud-connected backup & restore. I'm excited about this for multiple reasons i) I get to work on cloud storage full-time as opposed to a hobby, and they actually pay me for it ;-) ii) EVault is in San Francisco. Just between San Jose to San Francisco means such a sea change in the culture. iii) While I can't talk specifics, the project I'm working on is very ambitious and cutting edge!
Negatives of microservers for Swift:
Hopefully one of the above companies will like this use-case enough to run some real performance test to say one way or another in any conclusive manner, but superficially Cloud Storage systems such as Swift seem like a good target for a microserver architecture with wimpy cores.
Now to the topic of the post - there is tremendous industry buzz around the potential use of microserver CPUs (also called "wimpy" cores and most often associated with ARM SoCs) for datacenter applications as an alternative to traditional "brawny" x86 CPUs. These are a new class of light-weight power-efficient CPUs that promise to reduce power, real-estate, and cost while delivering the same aggregate performance. It may, of course, take multiple microserver CPUs to match the performance of one traditional CPU. But as we will see further in the post, that may not matter in scale-out architectures.
A microserver, therefore, is a new class of extremely low-power dense server. CPUs with wimpy cores have several common elements to them:
A microserver, therefore, is a new class of extremely low-power dense server. CPUs with wimpy cores have several common elements to them:
- Very low power for a given unit of performance
- Less number of lighter cores as compared to a typical Xeon or Opteron CPUs. Typically these processors also don't carry the burden of supporting legacy modes, full blown virtualization etc.
- System-on-a-chip (SoC) integration that eliminates an expensive chip-set
- Need not be based on x86 architecture – While brawny cores in servers are all based on x86, wimpy cores are mostly based on ARM (with the exception of Intel).
There's a ton of activity from the vendor side where companies such as AMD, Marvell, Calxeda, Cavium, NVidia, Samsung, Applied Micro, TI are working on enterprise class ARM SoCs; while Intel is working on similar Atom products. These companies either already have products or have announced plans for products in this category. The activity is so frenzied that wimpy cores might become a self-fulfilling prophecy!
There is also a lot of buzz from end-users e.g. this article demonstrates FaceBook's interest in wimpy cores http://www.businessweek.com/articles/2012-07-05/wimpy-cores-are-coming-to-facebook-dot-but-whose-cores.
However, these new lighter-weight CPUs are not a good fit for all workloads. If one were to broadly classify workloads as A) virtualized B) scale-up C) HPC and D) scale-out, microservers are best suited for scale-out computing. This is because scale-out workloads are typically simple, independent, homogeneous, but numerous and bursty. Scale-out computing is also based on a lot of open-source code which makes it easier to port to new server architectures e.g. ARM.
All of this combined would indicate that wimpy cores are a good fit for cloud storage systems such as Swift. As a reminder Swift is an open-source cloud storage project that is part of the OpenStack effort. But are microservers really a good fit here? Let's take a look:
Positives of a microserver architecture for Swift:
- OpenStack Swift is open-source and runs on Ubuntu. That's great for microservers since Canonical has taken a leadership role in porting Ubuntu to ARM.
- OpenStack Swift is indeed a scale-out architecture. This bodes well for microservers to be used here.
- There is a lot of flexibility in constructing the right compute : memory : storage ratio for OpenStack Swift. In fact one could argue that rather than sticking 24 drives behind a single or dual socket Xeon/ Opteron processor, it might actually be a lot more efficient to stick 4 drives behind one microserver CPU by providing compute a lot closer to storage. This architecture has the promise to reduce cost and power at the same time improving reliability and performance!
Negatives of microservers for Swift:
- Most microserver CPUs plan to have SATA interfaces and not SAS. This means the architecture is OK for SATA, but difficult to use for nearline SAS drives.
- By increasing the number of compute nodes, we are putting more strain on the network. This trade-off would have to be looked at.
Hopefully one of the above companies will like this use-case enough to run some real performance test to say one way or another in any conclusive manner, but superficially Cloud Storage systems such as Swift seem like a good target for a microserver architecture with wimpy cores.


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Mychal Kendricks unsure of Philadelphia Eagles future
- Updated: June 2, 2015
Mychal Kendricks said Tuesday that he's unsure if he will be with the Eagles when the 2015 season starts. Is the contract-year linebacker still on the trade block?
← Previous Story Chip Kelly's polarizing Philadelphia Eagles: My NFC East champs


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No Kid Hungry
Join Gobbles the Turkey at the Mile High United Way Turkey Trot this Thanksgiving. —Photo courtesy of Jen Morris
Our Top Charitable Picks for November 23-29
DCPA Holiday Cabaret: If you're not sure which show to catch at the Denver Center for the Performing Arts (DCPA) this month, this performance is your chance to get the best of all four. For the first time in DCPA history, cast members from all of the Center's current productions—The Lion King, A...
November 23 2015, 11:46 AM
Don't Miss: Julia Child Tribute and the 9th Annual Denver International Wine Festival
When Christopher and Darcy Davies, founders of Wine Country International magazine returned from a trip to the wine regions of Chile in 2004, they wondered why all of the great food and wine festivals in Colorado were all based in the mountains. They decided to change all...
November 5 2013, 10:30 AM


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ARLINGTON, Texas -- Josh Hamilton is back in the lineup after getting a diagnosis of ocular keratitis, which is the drying of the corneas. Read more about that here. But the bottom line is he's back in the lineup. And here are the lineups:
SS Stephen Drew (L)
DH Jonny Gomes
CF Yeonis Cespedes
1B Chris Carter
LF Brandon Moss (L)
RF Josh Reddick (L)
SS Cliff Pennington (S)
2B Ian Kinsler
SS Elvis Andrus
CF Josh Hamilton (L)
RF Nelson Cruz
LF David Murphy (L)
1B Mitch Moreland (L)


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AMP Agency Assumes Responsibility of SEO and SEM Services for Hasbro's Iconic Brands
Hasbro, Inc. a leading, global toy and game manufacturer, has selected AMP Agency, an integrated communications agency, as its Agency of Record for search services. Seeking an agency to further enhance their search efforts, Hasbro selected AMP as their search Agency of Record following a competitive review based on its creativity, innovative thinking and digital expertise.
"We were impressed with AMP's strategic approach to our business and ability to provide immediate innovation as well as their deep analytical understanding used to evaluate the success of our programs," said Victor Lee, Vice President Digital Brand Marketing, Hasbro. "We are excited about what AMP brings to the table for us today as well as their views of what's happening next in the digital media space."
AMP's Digital Services team will be responsible for providing digital strategy and implementation across multiple channels, including paid search (SEM), search engine optimization (SEO), creative and analytics. AMP and Hasbro aim to leverage an integrated mix of digital touchpoints to further connect consumers with Hasbro's family of iconic brands and products including: MONOPOLY, NERF, TRANSFORMERS, MY LITTLE PONY, G.I. JOE and PLAYSKOOL.
"We are honored to have the opportunity to work with such an innovative company and its roster of beloved brands," said Gary Colen, CEO of AMP Agency. "As a devoted Hasbro fan, I'm thrilled that AMP's team of digital experts will be able to assist Hasbro in fulfilling its goal of re-imagining, re-inventing and re-igniting its world class portfolio of brands."
About Hasbro
Hasbro, Inc. (NASDAQ: HAS) is a branded play company providing children and families around the world with a wide-range of immersive entertainment offerings based on the Company's world class brand portfolio. From toys and games, to television programming, motion pictures, digital gaming and a comprehensive licensing program, Hasbro strives to delight its global customers with well-known and beloved brands such as TRANSFORMERS, LITTLEST PET SHOP, NERF, PLAYSKOOL, MY LITTLE PONY, G.I. JOE, MAGIC: THE GATHERING and MONOPOLY. The Company's Hasbro Studios develops and produces television programming for markets around the world. The Hub TV Network is part of a multi-platform joint venture between Hasbro and Discovery Communications (NASDAQ: DISCA, DISCB, DISCK), in the U.S. Through the company's deep commitment to corporate social responsibility, including philanthropy, Hasbro is helping to build a safe and sustainable world for future generations and to positively impact the lives of millions of children and families every year. It has been recognized for its efforts by being named one of the "World's Most Ethical Companies." Learn more at www.hasbro.com
About AMP Agency
AMP Agency is an integrated communications agency that employs an "Insights Inspired, Results Driven" approach to conceive, develop and implement innovative marketing solutions across a range of channels. All recommendations AMP makes are inspired by consumer insights and a deep understanding of all media channels and powered by an agency-wide spirit of integration and creativity. Our client roster is comprised of industry leaders including Maybelline New York, Samsonite, Intel, Princess Cruises, NFL PLAYERS and Ansell Healthcare. For more information, visit us on the web atwww.ampagency.com.
Dynasil Corporation of America (NASDAQ:DYSL) is pleased to announce our new website is now available at www.dynasil.com
NWN Corporation Obtains Cisco's Elite Partnership Status In Their OpenDNS Certification Partner Program
NWN Corporation Named to CRN's 2016 IT Solutions Provider 500 List
ACT Government leads in waste innovation
Zenoss Names NWN Corporation as Managed IT Service Provider of The Year For Second Year In a Row
Datawatch Enables 450 CNAF Accountants to Prep Less and Analyze More
NWN Completes "Cisco Mega Audit" – Achieves Multiple "Master" Certifications
Best Practices for Pharmaceutical Waste
July 2016 – Mass Capital Announces a New Investment in The Cadmus Group, Inc.
April 2016 – Andrew DeLorey joins Mass Capital
March 2016 – Mass Capital announces a new investment in Valet Park of America
January 2016 – Mass Capital announces a new investment in Phillips Screw Company
November 2015 – Mass Capital announces a new investment in Triumvirate Environmental
September 2015 – Mass Capital announces a new investment in MedTouch
Mass. Capital Announces Addition to Investment Team


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- Unlined plaid stretch boardshort
- 11" rise, 21 1/2" outseam
- Tie waistband, hook and latch Superfly
- HyperPrene panels
- Model Info: Height: 5'10" | Chest: 38" | Waist: 33" | Hip: 39" | Wearing Size: 32
- 93% Polyester, 7% Elastane.
- Machine wash cold gentle cycle. Tumble dry gentle, low heat. Non-chlorine bleach when needed. Do not iron.
- Swimwear Return Policy: Swimwear must be accompanied by a receipt and returned within 30 days of purchase. Swimwear must have original tags.
- Made in Cambodia
SuperFreak boardshorts come with psychostretch, durable water resistant fabric, the Superfly.
HyperPrene Panels are Neoprene panels on this board short are ergonomically designed to give you that extra stretch where you needed.


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Property Details
Condo, 2 Bedrooms, 2 Baths, 4 Sleeps
Savor your morning coffee from your balcony as you witness an amazing sunrise over Boca Cieca Bay
Intercoastal Waterway. Or enjoy the gentle evening breezes and breathtaking view of the bay as you
sip your evening cocktail. The famous Don Cesar (pink hotel) glows with the beauty of the lavender
evening marking the Gulf of Mexico beaches for your exploring.
This 2br, 2bath condo is tastefully furnished with full amenities that include bed linens, towels,
etc, central air, TV, DVD, dishwasher, washer, dryer as well as reserved covered parking. This
corner unit is light and bright with fantastic water views from all rooms. The master bedroom is
furnished with a king sized bed and the second bedroom has a queen sized bed. Both bedrooms feature
their own private bathroom. You will truly feel like you are in paradise whether you are sitting on
your spacious balcony enjoying the view or swimming in the heated pool or jacuzzi located directly
on the water. You will enjoy this condo and all that Isla Del Sol has to offer!
Recent Review
Beautiful condo and views
About the Owner
5 Reviews
4.8/5 -
(5 traveler reviews)
Can't beat the views with this one!
We were taken aback when we walked in and saw the view! The condo had everything we needed including beach chairs, towels, and toys! We are now looking for our own place!
Thank you Linda & Jim!
Beautiful condo and views
This condo is VERY nice, clean, and has all the amities you would need. The location is so convenient to state park, beaches, restaurants, grocery and drug stores. The views are remarkable. Surrounded by water, you can see the Sunrise and Sunset, and watch the dolphins from the large 9th floor balcony. The grounds are immaculate and the property is well maintained. The owner, Linda, was such a pleasure to talk to, and made renting the unit so easy. The pictures do not capture the beauty of this property and all it has to offer. I would recommend this property over an above any of the other properties we have rented on Isla Del Sol in the past. We hope to go back in the near future and use this unit again.
Excellent Condo
Great location Fort Desoto on door step, best beaches in the USA Wide range of options iether to eat in or dine out).Spectacular views.Extremely comfortable and well equipped condo.
It's taken seven visits to the island to find a condo we really like.We found Linda Miller to be straight forward,honest and a caring owner.
We certainly would go back again.
Robert & Mary UK
Amazing Condo
This property was amazing! It was clean, well maintained and the view was beautiful from the balcony. We were even allowed a late check out due to our flight leaving in the evening. The property is also in close proximity to the beaches, shopping and restaurants. We would definitely recommend staying at this condo and if we ever return to Florida, we will stay there again.
9th floor paradise
This was our 6th year of renting a condo in Isla del Sol and our most favorite. The view from the 9th floor was outstanding and looked little different each time we looked out. The building had a lot of very friendly neighbors.
The condo was so comfortable! The pictures really don't do it justice. We would like to go back again.
Activity and Interests
Isla Del Sol is a private, tranquil island community of 350 beautifully landscaped acres off the
southern tip of St. Petersburg. It is just a mile from the white sandy gulf beaches,
Fort-de-Soto Park (rated best USA beach 2005), shopping, museums and restaurants in downtown St.
Pete and Pass-a-Grille with its unique shops and galleries.
Activities include fishing, swimming, golfing, boating, sight-seeing trips, nightlife, gaming
cruises, and watercraft rentals.
Busch Gardens is a short 45 minute drive. Disney World, Universal Studios, and Sea World are less
than 2 hrs away.
Rates & Availability
Rental rates
|Rate Period
|Weekend Night
|Monthly *
May 1 2017 - May 31 2017
30 night minimum stay
Jun 2 2017 - Aug 30 2017
7 night minimum stay
My Standard Rate
30 night minimum stay
* Approximate monthly rate. Actual rate will depend on the days of the month you stay.
Additional information about rental rates
|Refundable damage deposit
|condo assoc and cleaning
|$175 + Tax
|Tax Rate
Monthly Rate: $0 - $0
Jan, Feb, Mar $3100/month
April and Dec $2800/month
May, Oct, Nov $2200/month
June, July, Aug $1900/month
Please note: Add 12% Florida tax, $100 association fee and $125 cleaning/restock fee to the above
Bi-weekly rates will be considered during off season
Please call or email us. We'd love to share our little piece of paradise with you.
30 percent to secure booking, balance and security deposit due 30 days prior to arrival
Owner's Cancellation Policy
- 12
- Available arrival
- 12
- Available stay (no arrival)
- 12
- Unavailable
Property Type
- condo
- Air Conditioning
- Clothes Dryer
- Elevator
- Hair Dryer
- Heating
- Internet
- Iron & Board
- Linens Provided
- Living Room
- Parking ...
- Telephone
- Towels Provided
- Washing Machine
- Coffee Maker
- Dishes & Utensils
- Dishwasher
- Microwave
- Oven
- Pantry Items
- Refrigerator
- Stove
- Toaster
- Dining Area
- Books
- DVD Player
- Satellite / Cable
- Television
Pool / Spa
- Communal Pool
- Heated Pool
- Hot Tub
- Outdoor Pool
- children welcome
- non smoking only
- pets not allowed
Location Type
- Beach View
- Golf Course View
- Ocean View
- Waterfront
- restaurants
Leisure Activities
- antiquing
- beachcombing
- outlet shopping
- paddle boating
- scenic drives
- sight seeing
- sledding
- walking
Local Services & Businesses
- ATM/bank
- fitness center
- groceries
- laundromat
- massage therapist
- medical services
Sports & Adventure Activities
- cycling
- fishing
- golf
- mountain biking
- pier fishing
- rafting
- sound/bay fishing
- swimming
- water skiing
- water tubing
- wind-surfing
- Bathroom 1
- Bathroom 2
- 2 Bedrooms, Sleeps 4
- Bedroom 1
- Bedroom 2
- Balcony
- Guests provide their own meals
- Renters have full access to all resort amenities including a private waterfront pool and Jacuzzi, recreation room and fishing pier. An elevator provides direct access from the covered parking to the unit. The condo is fully equipped with linens and cooking utensils provided as well as coffeemaker, microwave, dishwasher, refrigerator with/ice maker, washer, dryer, phone, AC, TV and DVD


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Keep checking back to the SAAHP News on a weekly basis to see what's going on.
The Checkerboard Film Foundation, New York has donated 20 feature films on architecture to the School of Architecture, Art and Historic Preservation. The Foundation was established in 1979 to document, through the medium of film and video, artists who are making unique and important contributions to the American Arts.
Checkerboard Film Foundation
The team of Construction Management and Architecture students from Roger Williams University won the 2014 Design Build Institute of America (DBIA) Northeast Regional Competition. Team members include Brandon DaSilva (Team Captain) and Steve Pulver, Construction Management majors in the School of Engineering, Computing and Construction Management; and Marisa Bottai and Sam Froehlinger, Architecture majors from the School of Architecture, Art and Historic Preservation. The team's submittal, entitled Fourth Dimension, has won them a place in the national competition to take place in Fall 2014. Faculty coaches where professors Gokhan Celik and Fred Gould from Construction Management and Gary Graham from Architecture.
Tayo is a long time RWU Visual Arts Studies adjunct faculty member, whose work has been exhibited in solo shows most recently at the Phillips Collection, Washington DC, Reeves Contemporary, New York; CCNOA Project Room and H29 in Brussels, Belgium; and in group shows at URI, Newport Art Museum, Memorial Sloan-Kettering Cancer Center, New York; Chateau de Fernelmont, Belgium; Dieu Donne Gallery, New York; Baltimore Museum of Fine Art; the University of Wisconsin, RISD and many other venues. Art on Campus is curated by Rob Rustermier, Adjunct Faculty, who has led and c0-founded the program since its inception in 2011.
Tayo Heuser
The Fall 2014 Architecture Teaching Firms in Residence feature one returning firm and one new to the School, bringing nationally recognized practitioners to teach students in the Master of Architecture program.
designLAB, Boston is teaching an Arch 515 Graduate Architectural Design Studio focusing on a particularly close collaboration with the Providence Public Library, led by Robert Miklos, FAIA, Mary Ann Upton, AIA and Jason Van Yperen. designLAB is a widely noted firm named one of the US's top 50 architecture firms in 2011. Miklos was educated at RISD and the Harvard Graduate School of Design, Upton earned her Bachelor of Architecture degree from Rice University, Jason received his Master of Interior Architecture at RISD and a BA from Messiah College.
In conjunction with the University's Live Learn Community (LLC) initiative, a "Macro" LLC has been established this Fall 2014 to advance the Macro Center's Reforestation for Climate Change initiative. Bringing together students from four groups within the University's Building and Design themed communities, the Macro LLC is organized as an active corps of 125+ students, four RA's, faculty and deans undertaking field activities in collaboration with 200+ other RWU and community participants—LIVE--informed by knowledge gained from presentations, training sessions, readings, self-guided explorations and assessments—LEARN.
The Macro Center's Reforestation for Climate Change activities are organized at the Live Lean Community level by Resident Assistants Michael Aiardo, Rachel Foley, Nataly Maloney, and Gabriela Santostefano, with university, local, regional, national and international groups coordinated by Dean and Macro Center Director Stephen White.
The Center for Macro Projects and Diplomacy—the Macro Center--has taken up a major reforestation initiative in Rhode Island, mobilizing a corps of students in a multi-year effort aiming to achieve a measurable response to climate change. Following an initial test tree planting effort in Spring 2014 with RWU's American Institute of Architecture Students (AIAS) chapter and RI Tree Council Director John Campanini, the Macro Center worked further in the first week of the Fall 2014 semester through RWU's Community Connections Program for entering students.
Visual Arts Studies adjunct faculty member Julie Gearan has been chosen from 124 applicants to paint the official portrait of Rhode Island Governor Lincoln Chafee, as announced by the Rhode Island State Council on the Arts (RISCA). A final group of 14 applicants where presented to Governor Chafee for final consideration, from which Chafee chose Gearan. The portrait will be completed by 2015.
Julie Gearan Website
4th year student Conor Sturkhe has been awarded the 2014 Zachary Shapiro Scholarship, which is given annually to support an Architecture student studying abroad. Sturkhe is studying this Fall 2014 at Roger Williams University's Architecture Program in Florence, at the International Studies Institute.
ISI Florence
Graduate student Ellen Hassett and undergraduate Kristen Weigel have been awarded 2014 AIA Rhode Island Scholarships by the AIA Rhode Island Architectural Forum, the charitable arm of AIA Rhode Island. Hassett has been awarded $3100, and Weigel the $1000 DF Pray Scholarship. Weigel has also been awarded a $1000 2014 Industrial Fabrics Foundation Architecture Student Scholarship for the second consecutive year.
Sara Butler, Ph.D., Professor of Art and Architectural History and University Core Professor, has recently authored an article, "Reimagining the Movement: Beyond the Art of Negro Advancement at the Interior Building, 1937-1948," for the Summer 2014 issue of American Art, one of the US's leading arts publications. Butler wrote about Millard Sheets mural, The Negro's Contribution in the Social and Cultural Development of America: Science, at the Department of the Interior, Washington DC, and other works.


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This seedling has yellow foliage now. None last year and it is mostly on new
growth. Nothing has been added to soil or sprayed on it. It has grown
quite a bit with the lack of green. Smaller than most of it's siblings. It
was a seed last April.
Mike GreenfieldZone 5bSW Ohiohttp://home.cinci.rr.com/irisinohio/


text:
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It's cold out as I watch your breath turn into ice
Your words trembling so softly
Into the dawn I lie awake staring through my window half empty
Framed so perfectly through dark curtains
Flat against this wall
Your eyes are searching for someone, so I touch the glass screaming
You can't hear me
Think a little harder and you'll remember where you left me.
To be wrong again, I feel like crying on a cold winter's day.
Cast your shadow on a reflection of this presence,
Holding back nothing
Bound in chains that cannot break
Troubled, my soul is fleeing
A humble heart too afraid to shine, waiting silently
Heaving the loss of this strength
Instantly falling back again
Think a little harder and you'll remember where you left me.
To be wrong again, I feel like dying on a cold winter's day.
© Copyright 2017 Kynase. All rights reserved.
Status: Finished
Genre: Other
Great poem. I like the expression of emotion compared to a winter day.
Great poem. Its so sad it sounds like someone with a borken heart who just dont know what to say.
wow...i wanna sing it now!!ha ha..i might have to.i really like how you wrote it..it can be sung in many ways..or it could be read emotionally.very nice.^_^
Book / Fantasy
Book / Science Fiction
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Brooklyn, N.Y. (Thursday, Sept. 24, 2015) – Uprising Promotions light heavyweight Samuel Clarkson (16-3, 10 KOs) will be returning to ShoBox: The New Generation again on Friday, October 23rd, facing once-beaten Lavarn Harvell (15-1, 8 KOs). The bout will be his second appearance on ShoBox, with his last time being a third round stoppage of highly regarded prospect Jerry Odom in July.… Read Article
Atlantic City's Light Heavy weight Lavarn "Baby Bowe"Harvell on ESPN's Friday Night Fights from West Orange, N.J. on June 14th-
This past week I was given the opportunity to interview via telephone Atlantic City's rising light heavyweight prospect Lavarn Harvell as he prepares for his June 14th bout against Paul Vasquez from Martinez, California. … Read Article


text:
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I have used both Decleor and Murad for 20 years. Decleor is a beautiful aromatherapy French line for all skin types. Murad is for more specific, problematic skin issues such as severe acne and sun damage.
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For high investment yields that are worth the extra risk, look beyond junk bonds. Investors have piled into them, causing prices to rise and yields to fall to record lows. The BofA Merrill Lynch High Yield Master II index recently yielded just 5.7%.
Here's a better choice: a smaller, lesser-known investment class called business-development companies. Investors should stick with the safest ones, but even those yield about 8%. Go with Hercules Technology Growth Capital HTGC 1.2048192771084338% Hercules Capital Inc. U.S.: NYSE USD15.12 0.18 1.2048192771084338% /Date(1487973723359-0600)/ Volume (Delayed 15m) : 1516764 AFTER HOURS USD15.12 % Volume (Delayed 15m) : 100 P/E Ratio 16.085106382978722 Market Cap 1251799916.06243 Dividend Yield 8.201058201058201% Rev. per Employee 2652540 More quote details and news » (ticker: HTGC), Golub Capital BDC GBDC 0.78125% Golub Capital BDC Inc. U.S.: Nasdaq USD19.35 0.15 0.78125% /Date(1487973600335-0600)/ Volume (Delayed 15m) : 202650 AFTER HOURS USD19.35 % Volume (Delayed 15m) : 7 P/E Ratio 15.176470588235293 Market Cap 1068835961.623 Dividend Yield 6.614987080103359% Rev. per Employee N/A More quote details and news » (GBDC), New Mountain Finance nmfc 0.6756756756756757% New Mountain Finance Corp. U.S.: NYSE USD14.9 0.1 0.6756756756756757% /Date(1487973722351-0600)/ Volume (Delayed 15m) : 469284 AFTER HOURS USD14.9 % Volume (Delayed 15m) : 803 P/E Ratio 17.631049579931368 Market Cap 1751986623.31581 Dividend Yield 9.12751677852349% Rev. per Employee N/A More quote details and news » (NMFC), and Ares Capital arcc 0.0562429696287964% Ares Capital Corp. U.S.: Nasdaq USD17.79 0.01 0.0562429696287964% /Date(1487973600323-0600)/ Volume (Delayed 15m) : 2583992 AFTER HOURS USD17.89 0.1 0.5621135469364812% Volume (Delayed 15m) : 1863 P/E Ratio 11.781456953642385 Market Cap 7577490949.34785 Dividend Yield 8.544125913434513% Rev. per Employee 1206450 More quote details and news » (ARCC).
Business-development companies, or BDCs, make loans to small and midsize businesses and pass the interest on to shareholders as income. They trade like stocks, much like their pass-through investment siblings, real-estate investment trusts and master limited partnerships.
Even the safest of BDC stocks are offering yields of 8%. By contrast, junk bonds are generally yielding less than 6%. Stuart Goldenberg for Barron's
BDC loans and junk bonds both carry elevated risk of default, which portfolio managers seek to keep in check with diversification and careful choosing. There are important differences between the two assets, however. Many BDC loans are "senior secured" debt, meaning that in the event of bankruptcy, they must be paid before junk bonds. Also, many BDC loans carry floating interest rates. That puts them at lower risk of price declines if rates broadly rise.
In addition, BDCs have relatively little competition. Their customers are generally too small to tap the bond market, and commercial banks are finding such lending less attractive in the wake of new capital requirements.
The dearth of rivals means that BDCs can charge rates commensurate with their risks. Most charge fees to issue loans, similar to points on a mortgage. Once the loans become securities, demand for them rises among investors that can hold such loans but can't originate them. "We're able to originate loans by putting up 98 cents on the dollar, and then sell them at 101 cents," says Leonard Tannenbaum, chief executive of Fifth Street Finance FSC -0.43383947939262474% Fifth Street Finance Corp. U.S.: Nasdaq USD4.59 -0.02 -0.43383947939262474% /Date(1487973600148-0600)/ Volume (Delayed 15m) : 882611 P/E Ratio N/A Market Cap 647011004.785309 Dividend Yield 15.686274509803921% Rev. per Employee N/A More quote details and news » (FSC), a BDC. "Sometimes we sell them right away."
Little wonder that BDCs have been attracting more attention of late. Wells Fargo created a BDC index in January 2011. Soon after, UBS issued exchange-traded notes that track the index. This past February, Van Eck launched the group's first exchange-traded fund, Market Vectors BDC Income BIZD -0.1040582726326743% VanEck Vectors BDC Income ETF U.S.: NYSE Arca 19.2 -0.02 -0.1040582726326743% /Date(1487973589000-0600)/ Volume (Delayed 15m) : 76011 P/E Ratio N/A Market Cap N/A Dividend Yield 7.604166666666667% Rev. per Employee N/A More quote details and news » (BIZD). Rising recognition could eventually narrow the yield gap between BDCs and junk-bond funds. "I see an opportunity for valuation arbitrage," says Jonathan Bock, an analyst at Wells Fargo. "You pick up more than three extra percentage points versus junk-bond yields, and you get better protection."
Before buying into BDCs, however, investors should consider some of the potential pitfalls. Allied Capital and American Capital (ACAS) suffered high-profile blowups during the financial crisis of 2007-09. In hindsight, both companies had lent too aggressively while adding large amounts of stock exposure to their portfolios. BDCs use leverage, with a legal limit of 1:1, which amplifies the effects of excessive risk-taking. Since then, outside scrutiny of BDC loan books has risen, making today's valuations more reliable, says Bock.
Leverage can be a good thing, considering that many BDCs now can borrow at just over 6% (or 3.5%, if they use government-subsidized funds for small businesses) and lend at 10%. And many are prudent lenders; the group has managed to keep loan-loss rates at about 0.7% a year of capital deployed, below the rate of commercial banks.
BDCs must pay out the bulk of their income as dividends. Look for outfits that can cover at least three-quarters of their dividend payments with loan interest alone, before adding things like underwriting fees and gains, which can shrink in a downturn. Investor fees are important, too. Many BDCs charge like hedge funds, collecting 2% of assets plus 20% of winnings each year. Good ones have provisions that reduce management fees in the event of portfolio losses. Bad ones don't.
WELLS FARGO HAS divided the BDC universe into four tiers based on risk. Over the past two years, its top-ranked BDCs have gained a total of 36%, versus 24% for all BDCs in its coverage. Good bets from this lowest-risk group include Hercules Technology Growth, which specializes in tech companies and often negotiates for stock warrants as part of its deals. That gives it upside potential with less downside risk and mimics a tactic used by Warren Buffett in recent years. Golub Capital BDC is only three years old but is run by a firm with nearly two decades of lending experience. It has a shareholder-friendly fee structure and is particularly picky about risk. New Mountain Finance specializes in lending to companies whose earnings tend to hold up well during downturns in the economy.
Ares Capital is the largest BDC and a veteran of the financial crisis. Its shares plunged with those of Allied and American then, but with a crucial difference: Ares continued paying healthy dividends. The stock price has recovered, and while the ride for long-term holders has been anything but comfortable, it has been plenty profitable. The shares returned a total of 203% between their October 2004 debut and the end of last year. That compares with 50% for the Standard & Poor's 500 index.
Best Bets on BDCs
These stocks aren't the group's highest yielders, but they carry below-average risk.
| Recent
|Market Value (mil)
|Dividend Yield
|Dividend Coverage From Stable Cash Flow*
|Ares Capital/ARCC
|Golub Capital/GBDC
|Hercules Tech Growth/HTGC
|New Mountain Fin/NMFC
|*Measured by Wells Fargo. Ignores the contribution of fees, gains, and other income sources. At least 75% is ideal.
|Source: Thomson Reuters
E-mail: email@example.com


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Ashburn, United States Today, Mostly Sunny High 13° Low 9°
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> Leonidas Small Gift Basket
Leonidas Small Gift Basket
Leonidas' Holiday Gift Baskets are perfect for spoiling friends, family, and business associates during the holiday season.
This gift includes:
Leonidas General Assortment Ballotin (1/2 lb)
Napolitain Sampler - 6 flavors (24 pieces, .39 lbs)
Leonidas Dark and Milk Chocolate Bars(2 bars, 50 grams each)
Cello Sachet with Chocolate Fruits de Mer (8 pieces, .24 lb)
Hazelnut Chocolate Spread (Jar - 200g)
All the treats are carefully packed in a lidded wire hamper and decorated for the holidays.
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Summary | Excerpt | Reviews | Beyond the book | Readalikes | Genres & Themes | Author Bio
Prospero Taligent is mad. He is also the richest man in the world, an inventor, industrialist and entrepreneur who can do just about anything he likes. He lives in The Dream of Perpetual Motion's early 20th century alternate reality, where one can find technology as futuristic as flying mechanical men, and as retro as food automats and Victrolas. In the city of Xeroville, Prospero's madness seems to have tainted everyone, as evidenced by the need for "Shrink Cabs," which offer psychiatric therapy as well as a ride.
The story begins at the end; that is, we first meet Harold when he is already trapped aboard the Zeppelin, Chrysalis, where he can hear the voice of Prospero's daughter, Miranda, speaking to him, but cannot tell where she is. He suspects, but cannot prove, that the perpetual motion engine keeping the ship afloat is gradually running down. Harold refuses to speak to ...
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Innovative Italian shapewear available exclusively in the UK
Exclusive Italian shapewear that can help reduce cellulite with its innovative fibre weave. Get the perfect hour glass figure whilst simultaneously whilst increasing the breakdown of cellulite.
Shapewear sales are on the rise as TV personalities such as Gok Wan and Colleen Nolan have shown that beauty comes in all shapes and sizes and have taught self-conscious women everywhere how to promote and accentuate their most prominent features. FarmaCell Shapewear helps women to make the best of their body, and has 2 key benefits over its competitors.
Firstly, FarmaCell Shapewear uses tight elastic bands to further slim down the look of your thighs, hips, bottom and even your lower leg, helping you create the perfect hour glass look. Secondly, their shapewear comes with their patented Cellulite Cream. Tested at the University of Ferrara in Italy, it's been proven to enhance the microcirculation of small blood vessels, which helps to breakdown fatty deposits. But the Cellulite Cream becomes even more effective thanks to FarmaCell Shapewear's unique massaging weave, which increases circulation in your body and helps the Cream to delve deep into your skin for vigorous cellulite breakdown.
"This product is great for anybody who is looking for a highly effective and proven treatment for cellulite, as well as a comfortable way to tone their figure," says WeightWorld Manager Clint Garnier. "FarmaCell Shapewear is the only shapewear range that enhances your body both inside and out."
The response from our customers has already been hugely positive. One customer commented: "This material feels fantastic on the skin. It's one of the only outfits that truly work. It's knocked inches off my thighs already."
The technically advanced and clinically proven Farmacell Micro-Massaging Shapewear and Cellulite Cream is exclusive to Weightworld.co.uk – a leading online weight management store that provides its customers with professional advice and high-quality products to help them maintain a healthy lifestyle.
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Comfort Click Ltd is an umbrella company that runs several e-commerce websites, one of which is WeightWorld.co.uk - a leading onlibe weight management store that offers helpful advice and quality products to its customers to help maintain a healthy lifestyle
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Backpacking / Europe / Hosteling / Travel / Travel Tips
A Traveler's Journal: Meandering Budapest's Streets
With its origins dating back to the 9th century, Budapest was officially formed in 1873 when the cities of Buda, Pest and Obuda were unified. Once hailed as the heart of Europe and the Pearl of the Danube, it can be easy to overlook Budapest's rich and influential history.
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