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PML nuclear bodies (NBs) are nuclear structures that have been implicated in processes such as transcriptional regulation, genome stability, response to viral infection, apoptosis and tumor suppression. PML has been found to be essential for the formation of the NBs, as these structures do not form in Pml null cells, while PML add back fully rescues their formation. However, the basis for such a structural role of PML is unknown. We demonstrate that PML contains a SUMO binding motif that is independent of its SUMOylation sites and is surprisingly necessary for PML-NB formation. We demonstrate that the PML RING domain is critical for PML SUMOylation and PML-NB formation. We propose a model for PML-NB formation whereby PML SUMOylation and non-covalent binding of PML to SUMOylated PML through the SUMO binding motif constitutes the nucleation event for subsequent recruitment of SUMOylated proteins and/or proteins containing SUMO binding motifs to the PML-NBs.
The PML tumor suppressor gene was cloned at the breakpoint of the t(15;17) chromosomal translocations of acute promyelocytic leukemia (APL) where it fuses to the retinoic acid receptor α gene (RARα) leading to the generation of an oncogenic PML-RARα chimeric protein (Pandolfi, 2001). The PML gene encodes multiple isoforms, which are derived from alternative mRNA splicing. PML nuclear isoforms are localized in distinct subnuclear structures known as PML-nuclear bodies (PML-NBs), PML oncogenic domains (POD), nuclear domain 10 (ND10), or Kremer (Kr) bodies (Hofmann and Will, 2003; Jensen et al., 2001; Zhong et al., 2000b), although cytosolic isoforms of PML have also been characterized and found to be functionally relevant (Bernardi and Pandolfi, 2003; Lin et al., 2004). The PML-NB is disrupted in APL blasts as a consequence of the dominant negative action of the PML-RARα fusion protein over PML through direct physical interaction, while PML-NB reorganization correlates with biological and clinical response upon treatment with retinoic acid (Koken et al., 1994). PML has multiple critical tumor suppressive functions, which are impaired in APL cells and in cancer cells lacking PML and PML-NBs (Gurrieri et al., 2004; Salomoni and Pandolfi, 2002).
A major advance in the current understanding of the PML-NB structure is derived from a thorough characterization of its protein, rather than nucleic acid, composition, though PML-NBs make connections with surrounding chromatins (Boisvert et al., 2000). To date more than forty proteins involved in essential cellular processes such as DNA damage response and repair, apoptosis and transcriptional regulation have been found to colocalize with PML in the PML-NBs (Hofmann and Will, 2003; Jensen et al., 2001; Zhong et al., 2000b).
A correlative microscopy and electron microscopy analysis in serial thin sections revealed the donut-shaped PML-NB structure (Boisvert et al., 2000), while the PML-NB dynamically changes its morphology during the cell cycle and in response to cellular stresses (Bernardi et al., 2004; Dellaire et al., 2006; Everett et al., 1999). We and others, using Pml knockout mice and cells, established that PML is the primary essential component of PML-NB, and that PML SUMOylation is required for PML-NB formation (Ishov et al., 1999; Wang et al., 1998; Zhong et al., 2000a; Zhong et al., 2000b). Interestingly, many of the proteins found in the PML-NBs are SUMOylated (Seeler and Dejean, 2003). Furthermore, components of SUMOylation machinery are also localized in PML-NB (Seeler and Dejean, 2003). However, to date no plausible model has been proposed to explain why PML plays such an essential structural role in the formation of the PML-NB and why PML needs to be SUMOylated to exert its function.
Since functional PML-NBs can be reconstituted in Pml−/− mouse cells by introducing wild type (wt), but not SUMOylation deficient PML (Ishov et al., 1999; Lallemand-Breitenbach et al., 2001; Zhong et al., 2000a), we have utilized this strategy to determine the requirements for PML-NB formation, and, on the basis of these findings, we now propose a new model for the PML-dependent nucleation and formation of the NB.
SUMO1 was originally identified as a PML interacting clone 1 (PIC1) by yeast two hybrid screening (Boddy et al., 1996), however PML SUMOylation was not discriminated from a possible non-covalent interaction between PML and SUMO1. We reasoned that if such non-covalent interaction between PML and SUMO1 does in fact take place it might be important for PML-NB formation. To resolve this issue, we generated a SUMOylation deficient version of PML IVa (Jensen et al., 2001) by mutagenizing lysines at positions 65, 160, 490 to arginines (a mutant which we termed PML3m; Figure 1B). We next transfected PML3m in Pml−/− mouse embryonic fibroblasts (MEFs) with or without GFP-SUMO1 and performed immunoprecipitation followed by Western blot analysis. Indeed, PML3m was still efficiently pulled down by GFP-SUMO1 (Figure 1C). Similar results were observed in reciprocal immunoprecipitations as well as in other cell types (Pml+/+ MEFs or 293T cells, see Supplementary Figure S1). These results suggested that PML retains a SUMO binding domain that is independent of its SUMOylation sites. During the course of this study, Song et al. reported a SUMO binding consensus sequence (VVVI) identified by NMR characterization of peptides from various proteins (Song et al., 2004). PML shares this consensus at position 508VVVI511 (Figure 1A), which is localized in exon 7 and thus is present in all PML isoforms except PML VIIb (Jensen et al., 2001). We thus mutated the putative SUMO binding motif from large non-polar (VVVI) to small non-polar amino acids (AAAS) in PMLwt or PML3m, or deleted this motif in the context of PML3m. These plasmids were once again cotransfected with GFP-SUMO1 in Pml−/− MEFs and immunoprecipitation and Western blot analysis were performed. We found that mutation or deletion of the VVVI sequence from PML3m abrogated the ability of GFP-SUMO1 to interact with PML3m (Figure 1D). Similar results were obtained in experiments performed in 293T cells and Pml−/− MEFs using different antibodies for immunoprecipitation and Western blot analysis (Supplementary Figure S2). The VVVI consensus in PML is therefore required for SUMO binding.
To determine whether the SUMO binding motif in PML directly mediated the interaction of PML with SUMO, we generated synthetic PML-derived peptides containing a wild type or mutagenized SUMO binding motif (VVVI) and tested whether they would interact with purified GST-SUMO1. Indeed, amino acid changes within the motif compromised SUMO binding activity (Figure 1E). In agreement with these findings, PMLas displayed a reduced SUMO binding activity when compared to PMLwt in in vitro binding assays utilizing purified recombinant His-tagged PMLwt or its mutants as well as GST-SUMO1 and GST (Figure 1F). Taken together, these experiments demonstrate that the SUMO binding motif in PML can directly interact with SUMO.
We also added to the analysis a PML RING domain mutant (PMLcs) in which we mutagenized the key cysteine residues to similar small non-polar amino acid serines (57C/S, 60C/S) in order to disrupt the RING structure (Figure 1A). A similar mutagenesis strategy was previously utilized to demonstrate the critical role of zinc coordinating amino acids (cysteine or histidine) in the ability of the RING finger structure to retain self-ubiquitination activity in a group of otherwise functionally unrelated proteins (Lorick et al., 1999). We observed that RING domain mutant PMLcs displayed reduced SUMO binding ability (Figure 1F). To further explore the role of RING-mediated SUMO binding, we generated a RING domain mutant in a SUMOylation deficient PML background (PML3mcs) and tested its SUMO binding activity in Pml−/− MEFs. We found that, in vivo, PML3mcs displayed a reduced interaction with SUMOylated high molecular weight proteins when compared to PML3m, but not a defect as severe as the one observed with the SUMO binding motif mutant PML3mas (Supplementary Figure S3). Collectively, our data suggest, but do not prove, that the PML RING domain can also mediate SUMO binding. We cannot rule out the possibility that the subtle nonspecific structural changes caused by RING domain mutation may result in reduced SUMO binding although we demonstrated that the RING domain mutant PML is folded properly to be able to dimerize with PMLwt in vivo (Figure 5, see below). In light of our in vivo observations (Supplementary Figure S3; Figure 5, see below), we propose that the SUMO binding motif largely contributes to interactions of PML with other SUMOylated proteins in vivo, while the SUMO binding ability of the PML RING domain would mediate a possible enzymatic activity such as PML auto-SUMOylation or the SUMOylation of other proteins, in turn, indirectly, exerting its influence in the binding of PML to other SUMOylated proteins (see below).
We next tested whether the PML SUMO binding motif is required for PML-NB formation. To this end, plasmids expressing PML and the PML mutants described above were cotransfected with GFP-SUMO1 in Pml−/− MEFs and confocal immunofluorescence analysis was performed (Figure 2). As expected, cells transfected with PMLwt contained 10–20 PML-NBs per cell nucleus of heterogeneous sizes, and PMLwt was found to completely colocalize with GFP-SUMO1. PMLcs displayed predominantly a nucleoplasmic pattern, with a few dots colocalizing with GFP-SUMO1. The PML SUMO binding motif mutant (PMLas) formed fewer and aberrant structures compared to those formed by PMLwt in the nucleus, but still colocalized with GFP-SUMO1 in agreement with the notion that it can still be SUMOylated. PML3m also formed fewer aberrant compact structures compared to those formed by PMLwt in the nucleus and still colocalized with GFP-SUMO1, in agreement with the notion that it does retain the SUMO binding motif. Strikingly, however, mutagenesis or removal of the SUMO binding motif in PML3m (PML3mas, PML3mds, respectively) completely abrogated colocalization with GFP-SUMO1, although, intriguingly, PML3mas (or PML3mds) and GFP-SUMO1 were found to accumulate in adjacent subnuclear regions. By contrast, GFP-SUMO1 alone displayed a diffused nuclear staining pattern since SUMO1 mostly accumulates in PML-NBs and in Pml−/− cells PML-NBs do not form. Furthermore, we confirmed the requirement of the SUMO binding motif and RING domain in PML-NB formation by visualizing endogenous PML-NB proteins such as SUMO1 and Daxx (Supplementary Figure S4, S5). We also determined that the structures formed by PML or its mutants are distinct from nucleoli or splicing speckles (Figure 3; Figure 4). Collectively, this analysis demonstrates that the PML SUMO binding motif and an intact RING domain are required for PML-NB formation.
To explore the mechanism underlying the involvement of the PML RING domain in PML-NB formation, we first compared the levels of SUMOylation of the PML RING mutant, PMLcs, to those of PMLwt or the PML SUMO binding motif mutant, PMLas. Co-transfecting these mutants with GFP-SUMO1 and then performing immunoprecipitations to enrich the SUMOylated proteins, we found that the SUMOylation of PMLcs was drastically reduced while SUMOylation of PMLas was almost normal when compared to PMLwt (Figure 5A and 5B). To rule out the possibility that RING mutagenesis impaired PML homo-dimerization ability, PMLwt and PMLcs differently tagged (PMLwt Xpress-tagged or FLAG-tagged, and PMLcs FLAG-tagged) were cotransfected in Pml−/− MEFs and their ability to interact was assessed by immunoprecipitation and Western blot analysis. The result indicated that PMLcs displays (homo)dimerization abilities similar to the ones of PMLwt (Figure 5C). Because the RING domain is essential for the activity of a class of SUMO E3 ligases (Seeler and Dejean, 2003), and only PML (or its mutants) and GFP-SUMO1 were over-expressed in Pml−/− MEFs in these experiments, it is tempting to speculate that PML acts as a SUMO E3 ligase that catalyzes self-SUMOylation. Indeed, in agreement with this possibility, the PML RING domain was shown to be essential for PML self-SUMOylation in budding yeast, where PML or its RING domain mutant (72C/A) and SUMO1 were over-expressed (Quimby et al., 2006).
Based on our biochemical and cytomorphological analysis, we propose a new model for the formation of the PML-NB (Figure 6). In mitosis, PML is found in aggregates. This is due to its de-SUMOylation, subsequent disassembly of the PML-NBs (Everett et al., 1999), and PML dimerization/multimerization mediated by its RBCC motif (Jensen et al., 2001). In interphase, PML is SUMOylated and, importantly, once PML is SUMOylated, the PML SUMO binding motif can now mediate interactions with nearby SUMOylated PML molecules hence allowing the formation of orderly PML networks. Since a large number of proteins associated with the PML-NB are SUMOylated and/or do contain SUMO binding motifs (e.g. Daxx; see the accompanying paper by Lin et al.), it is possible that these proteins are recruited to the PML networks to eventually form higher order of protein structures such as the PML-NB through non-covalent interactions mediated by covalently bound SUMO and SUMO binding motifs present in PML and these other NB components. In agreement with this model and with the essential roles for PML SUMOylation and subsequent non-covalent binding through the SUMO binding motif, an unSUMOylatable PML is found in aggregates in the interphase nucleus, while a PML mutant that can be efficiently SUMOylated, but does not retain a functional SUMO binding motif, cannot form normal PML-NB either. This is in agreement with the notion that in both cases the formation of higher order of protein structures such as the PML-NB is prevented.
We found that SUMO binding motif or RING domain PML mutants are defective in apopotosis when expressed in Pml−/− MEFs (Supplementary Figure S6), suggesting that the tumor suppressive activity of PML may depend, at least in part, on its ability to mediate the proper formation of the PML-NBs, an idea that can now be thoroughly tested in PML mutant knock-in mice.
Our findings have implications for the pathogenesis of APL. As mentioned above, the PML SUMO binding motif is localized in PML exon 7, which is present in most PML isoforms and is invariably lost in the PML-RARα oncoprotein of APL as the chromosomal translocation breakpoints always lay in 5′ introns (3 and 6 respectively; see also Figure 1A). Loss of SUMO binding motif in the PML-RARα fusion protein may explain why the fusion protein fails to aggregate in NB-like structures (e.g. when transfected in Pml−/− MEFs, data not shown), acting instead as a dominant negative PML mutant in triggering the disruption of the PML-NB in the APL blast (Koken et al., 1994).
Similar SUMO binding motifs may be present in many proteins involved in SUMOylation related processes (Hannich et al., 2005; Minty et al., 2000; Reverter and Lima, 2005; Song et al., 2004). From a biological stand point, it is tempting to speculate that the non-covalent interaction mediated by SUMO binding motifs, by allowing protein-protein interaction to occur, may represent a general mechanism that could modulate a number of diverse functions, including the assembly of macromolecular structures such as PML-NBs, as well as the SUMOylation process itself, in analogy with ubiquitin/substrate non-covalent interactions (Gill, 2004).
PML used in the study was classified as PML IVa (Jensen et al., 2001). It was cloned into BamH I and EcoR I sites of pCMVtag2B vector (Stratagene) so that a FLAG tag was placed at the PML N-terminus. PML3m, PMLcs, PMLas, PML3mas, and PML3mds were constructed from the cloned FLAG-PML IVa plasmid using a Quikchange Site-Directed Mutagenesis Kit (Stratagene). Plasmids expressing His-tagged PML I mutants were generated by using a Quikchange mutagenesis based on pET33b-PML I from S. Bergmann. The plasmid expressing recombinant GST-SUMO1 was constructed by insertion of a PCR product of activated SUMO1 (C-terminal ended as two glycines) into BamH I and Xho I sites of pGEX-5X-1 (Amersham Biosciences). Retroviral vectors that express FLAG-PMLwt and FLAG-PML3m were constructed by insertion of respective PCR products into BamH I and EcoR I sites of a pBABE vector, and other mutants were subsequently made by Quikchange mutagenesis. All plasmids were verified by DNA sequencing. Primer sequences are available upon request. The pGFP-SUMO1 plasmid was a kind gift of P. Salomoni. Xpress-PML was previously described (Lin et al., 2004).
Human 293T cells, Pml+/+ immortalized mouse embryonic fibroblast (MEFs), and Pml−/− immortalized MEFs were maintained in Dulbecco's minimal essential medium (DMEM) supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamate and antibiotics (1% v/v penicillin-streptomycin). Cells were split one day before transfection in 6 well plates (for 293T cells) or in 10 cm plates (for MEFs) so that cells reach to ~70% confluency upon transfection using effectene transfection reagent (Qiagen) according to supplier's protocol. At 36–48h after transfection, cells were rinsed with cold phosphate buffered saline (PBS), and scraped in 1 ml of cold PBS with 10 mM N-ethylmaleimide (NEM, Sigma). Cell pellets were lysed at 4 °C in 200 μl of RIPA buffer (1% NP40, 0.5% DOC, 0.1% SDS in PBS) with freshly added 10 mM NEM, protease inhibitor (Roche, 50 ml/tablet) and 1 mM phenylmethylsulfonyl fluoride (PMSF, Sigma). The cell lysates were then sonicated on ice for 3 sec and centrifuged at maximum speed for 10 min at 4 °C in an Eppendorf centrifuge. The supernatants were mixed with 10 μl of washed and packed protein G sepharose beads (Amersham Biosciences) at 4 °C for 30 min. The cleared supernatants were then mixed with 10 μl of washed and packed protein G sepharose beads and 1 μl of anti-GFP (rabbit polyclonal antibody, Clontech), or anti-FLAG antibodies (Sigma) at 4 °C overnight. The beads were washed 4 times with RIPA buffer and the immunoprecipitates together with inputs were analyzed by 8% SDS-PAGE. The stacking gels were always saved for the detection of high molecular weight SUMOylated proteins. Western blotting was performed using anti-GFP (mouse monoclonal antibody, 1:1000, Clontech), or anti-FLAG antibodies (1:1000), visualized by enhanced chemiluminescence (Amersham Biosciences).
His-tagged Recombinant PML and mutants were produced in Rossetta 2(DE3)pLysS cells (Novagen) upon induction with 0.5 mM IPTG (Sigma) at 37 °C for 4 h. The proteins were purified using Talon magnetic beads (Dynal Biotech) according to the supplier's procedure. Recombinant GST-SUMO1, and GST were produced in BL21 cells (Novagen) upon induction with 0.5 mM IPTG (Sigma) at 37 °C for 4 h. The proteinswere purified using glutathione-sepharose beads (Amersham Biosciences) according to the supplier's protocol. The eluted proteins were dialyzed, concentrated by Centriprep 10 (Millipore), aliquotted and stored at −80 °C until use. The purity and concentration of purified proteins were analyzed by SDS-PAGE and Coomassie blue staining with known amount of bovine serum albumin (BSA) as a control. PML peptides were designed to contain an N-terminal biotin and tetrapeptide linker SGSG followed by 17 mers of PML centered at the SUMO binding motif VVVI, or its mutants in which VVVI was replaced with AAAS or VVKI. The peptides were synthesized and HPLC purified by the Protein Core facility at MSKCC.
For the in vitro binding assay using synthetic peptides, 3 μg of recombinant GST-SUMO1 or GST were incubated with 15 μg of biotinylated PML peptide or its mutant peptides at 4 °C overnight in 300 μl of interaction buffer containing 20 mM Hepes, pH 7.9, 150 mM KCl, 5 mM EDTA, 0.5 mM DTT, 0.1% (V/V) Nonidet P-40, 0.1% (W/V) BSA, 1 mM PMSF, 10% glycerol. Then 20 μl of streptavidin beads (Pierce) was added and incubated for 1 h. After three washes with PBS, the bound proteins were determined by Western blot analysis using antibodies against GST (1: 1000, Sigma) or SUMO1 (1: 500, Santa Cruz Biotechnology).
For the in vitro binding assay using full length recombinant proteins, 1 μg of purified His-tagged PML or its mutants was incubated with equivalent amounts of purified GST-SUMO1 and GST in 20 mM Hepes, pH7.5, 150 mM NaCl, 0.1% Tween-20 at 4 °C for 4 h. Then the mixtures were mixed with 10 μl of washed Talon magnetic beads with rotation at 4 °C for 1 h. The input and unbound fractions, together with the bound fractions after three washes, were analyzed by Western blot using antibodies against His (1:1000, Sigma) or GST (1:1000, Sigma). The intensities of His-tagged PML and GST-SUMO1 bands in the bound fractions were quantified using MacBAS V2.5-1 software and the ratios of GST-SUMO1 to PML calculated.
Pml−/− MEFs were grown in slide chambers and transfected with PML or its mutants and GFP-SUMO1 using effectene transfection reagent (Qiagen) according to the supplier's protocol. After 36–48h, the cells were washed with PBS and fixed in 4% paraformaldehyde at RT for 20 min. The cells were then permeabilized in PBS containing 10% FCS and 0.1% Triton X-100. Rabbit anti-PML antibodies (PGM3, Santa Cruz Biotechnolgy) were added (1:500) and incubated in the same buffer at RT for 1 h, followed by incubation with goat anti-rabbit IgG conjugated with Alexa fluor 568 (Molecular Probes, 1:500), together with DAPI (0.1 μg/ml) at RT for 30 min. After washes, the slides were mounted. Images were captured by laser-scanning microscope (Leica) and processed in Adobe Photoshop 7.0. For the detection of endogenous protein markers, GFP-SUMO1 was omitted in transfections. These proteins include: SUMO1 (1:50, Santa Cruz Biotechnolgy), Daxx (1:25, Santa Cruz Biotechnolgy), Fibrillarin (1:100, Abcam), and SC35 (1:100, Abcam). They are all polyclonal antibodies and were used with anti-rabbit antibodies conjugated with Alexa fluor 488 (Molecular Probes, 1:500).
Phoenix cells grown on 10 cm lysine coated plates (Biocoat Inc.) at 70% confluency were transfected with retroviral vectors expressing FLAG-PMLwt or its mutants using Lipofectamine 2000 (Invitrogen) according to the supplier's protocol. After 48 h, the viruses containing media were collected, passed through a 0.45 μM filter, and directly used in the infection of Pml−/− MEFs for 48 h in the presence of 4 μg/ml of polybrene (Sigma). Infected cells were then selected for one week in the presence of 2.5 μg/ml of puromycin with 2–3 medium changes. The resulting cells were seeded on 6 well plates at 70% confluency. After 48 h, the cells were collected in PBS by trypsinization, together with dead cells in the medium. The cells were washed with PBS, fixed in 70% ethanol at 4 °C overnight. After washing with PBS, the cells were stained in PBS containing 2 μg/ml of propidium iodide (PI, BD Biosciences) and 0.5 μg/ml of DNase-free RNase (Roche) at RT for 1 h. The PI stained cells were subjected to Fluorescence Activated Cell Sorting (FACS) analysis. The results are presented in log scale to magnify the sub-G1 population of cells.
We thank R. Bernardi, L. Trotman, S. Bergmann, Z. Chen and other members of the Pandolfi laboratory for helpful discussion, advice and reagents. We greatly appreciate S. S. Yi and P. Tempst for help with peptide synthesis and J. Clohessy for assistance with the FACS analysis. We also thank I. Guernah for providing MEFs, T. Tong, R. Huq and other members in Molecular Cytology Core Facility for their assistance with imaging. We are grateful to L. DiSantis for editing and critical reading of the manuscript. This work was supported by the NIH/NCI grant (R01-CA74031) to P.P.P. The authors declare no conflicts of interest associated with the study. Correspondence and requests for materials should be addressed to P.P.P. (email: gro.ccksm.iks@iflodnap-p)
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For women, estate planning means protecting loved ones and their own retirement security, too.
An inner-city neighborhood can be a good, and affordable, choice for retirement. Here are 10 top spots.
Surprisingly, the pursuit of retirement happiness takes work–plus structure, community and purpose.
From a pill-bottle cap that calls your druggist to GPS locator shoes, high-tech ideas to help seniors are proliferating.
Some boomers have a tough problem: parents who resist the help they need to stay safe.


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With the peak holiday spending in their rearview mirrors, Nevada retailers say they are cautiously optimistic about continued growth in 2017. "Retail is trending positive. I'm conservatively bullish and would be happy with one to two percent growth next year," said Kevin Budny, senior general manager of the Galleria at Sunset in Henderson. "We were comparable to 2015 this … [Read more...]
Office Summary: Second Quarter 2016
Southern Nevada Las Vegas Valley's speculative office vacancy rate in Q2 2016 declined 2.5 points to 16.2 percent from Q1 2016. This was a big decline after the first rise in vacancy in four quarters during Q1. Vacancy rates ranged from 14.1 percent for Class C space to 21.3 percent for Class A space at the end of Q2. There was one office completion in Q2, the Park at … [Read more...]
Retail Summary First Quarter 2016
Southern Nevada The Las Vegas Valley retail vacancy rate dropped 0.2 percentage points to 10.1 percent in Q1, 2016, a positive start to the year. At the end of Q1, Southern Nevada's vacancy rate was again lowest in power centers at 7.2 percent. Neighborhood and community centers were almost even with vacancy rates of 10.9 and 11 percent, respectively. As of the end of Q1, … [Read more...]
Industrial Summary: Fourth Quarter 2015
Southern Nevada The Las Vegas Valley's overall industrial vacancy rate in Q1, 2016 was 5.0 percent, a rise of 0.3 points, from 4.7 percent in Q4, 2015. Q1's increase was the second quarter in a row with an increase in vacancy rate. Still, with the vacancy rate at such a low level, these rises are not a cause for concern. The strong growth in demand for industrial space, along … [Read more...]
Office Summary: Fourth Quarter 2015
Southern Nevada The Las Vegas Valley spec office vacancy rate in Q4, 2015 declined 0.1 points to 18.4 percent from Q3, 2015. Compared to Q4, 2014, the office vacancy rate is down 0.5 points, from 18.9 percent. Q4's vacancy rate drop means that the Valley's office market has improved for four straight quarters. Vacancy rates ranged from 13.3 percent for Class C space to 27.1 … [Read more...]
Retail Market: Nevada's Retail Shows Slow, Steady Improvement
The retail component of Nevada's commercial real estate industry continues to recover, with vacancy rates slowly dropping. Southern Nevada In the Silver State's southern region, the retail sector shows "moderate recovery," said John Restrepo, principal of RCG Economics, a Las Vegas-based firm providing market trend analysis and economic research. Lower gas prices have left … [Read more...]
Office Summary: Third Quarter 2015
Southern Nevada Las Vegas Valley "spec office" vacancy rates during Q3, 2015 declined 0.3 points to 21.3 percent from Q2, 2015. Compared to Q3, 2014, the office vacancy rate is down 0.3 points, from 21.6 percent. Q3's vacancy rate-decline means that the Valley's office market has been improving for seven of the last eight quarters. Vacancy rates ranged from 15.2 percent for … [Read more...]
Office Summary: Second Quarter 2015
Southern Nevada Las Vegas Valley spec office vacancy rates during Q2, 2015 ranged from 16.3 percent for Class C space to 32.1 percent for Class A. The overall vacancy rate was 21.6 percent, a decline from 21.7 percent recorded in Q1, 2015. Compared to Q2, 2014, the vacancy rate is down 1.1 points from 22.7 percent. Q2's decline in vacancy rate makes 6 quarters with an … [Read more...]
Retail Summary: First Quarter 2015
Southern Nevada The Las Vegas Valley anchored retail vacancy rate rose 0.5 points from 11.4 percent in Q4 2014 to 11.9 percent in Q1 2015. At the end of Q1, vacancy rates ranged from 9.3 percent in Power Centers to 12.7 percent in both Community and Neighborhood Centers. The first quarter makes two consecutive quarters with a rising vacancy rate. Still, this increase is not … [Read more...]
Office Summary: First Quarter 2015
Southern Nevada Las Vegas Valley office vacancy rates during the first quarter of 2015 ranged from 16.5 percent for Class C space to 29.3 percent for Class A space. The overall vacancy rate was 21.7 percent, a slight rise above the 21.3 percent recorded in quarter four of 2014. Q1's rise equates to the first increase in vacancy rate in 6 quarters. Completions for Q1 were … [Read more...]
Industrial Summary: First Quarter 2015
Southern Nevada The overall vacancy rate for the Las Vegas Valley industrial market in Q1, 2015 was 6.9 percent, a drop of 0.3 points, from 7.2 percent in Q4, 2014. The first quarter's decline equates to nine straight quarters of declining industrial vacancies. The strong growth in the demand for industrial space, paired with resurgent construction, shows that the Valley's … [Read more...]
Nevada Taxes Reaching Deeper
Hall of Fame baseball player Yogi Berra once said it is deja vu all over again. He wasn't wrong if he was describing Nevada politics and biennial efforts to create a tax code that can better withstand economic recessions. Nevada lawmakers have recently been debating another proposed tax increase geared towards adding funds to the state's woeful educational system. In total, … [Read more...]
Retail Summary: Fourth Quarter 2014
Southern Nevada The overall vacancy rate for the Las Vegas Valley anchored retail market in the fourth quarter of 2014 was 11.4 percent. The vacancy rate increased 0.4 points, from 11 percent in the third quarter. However, vacancies were down 0.3 points, from 11.7 percent compared to the same time in 2013. While the vacancy rate rose, the general trend still shows that the … [Read more...]
NAIOP Southern Nevada Chapter Announces New Officers, Board of Directors and Committee Chairs for 2015
Las Vegas –NAIOP Southern Nevada chapter -- a trade organization representing commercial real estate developers, owners and related professionals in office, industrial, retail and mixed-use real estate in Southern Nevada -- has announced its 2015 officers, board of directors, committee chairs, and liaisons. The 2015 NAIOP Southern Nevada officers are as follows: … [Read more...]
Industry Focus: Commercial Real Estate
As the economy recovers, commercial real estate is slowly regaining footing in the Silver State. Recently, executives representing the industry met at the Las Vegas offices of Gordon Silver to discuss the challenges of commercial real estate in Nevada. Connie Brennan, publisher of Nevada Business Magazine, served as moderator for the event. These monthly meetings are … [Read more...]
Nevada Land Market Continues to Recover
Demand for land in Nevada has resembled a rollercoaster pattern this decade. During the real estate boom in the first half of the 2000s, both residential and commercial developers hungrily purchased land, driving up prices. With the recession, the opposite happened; development nearly ceased, land demand decreased and so did the price. Again, the pendulum has begun to swing … [Read more...]
Builders and Developers: Bullish on Nevada
Nevada's skyline is once again changing. Commercial real estate, which saw many projects stalled mid-recession, is finally moving: contractors are hiring and builders are returning. But is there activity across the board in all industry sectors – industrial, office, retail, mixed-use – or are some moving faster than others? Does it spell recovery? Or another boom and bust … [Read more...]
Power Poll 2014: The Bounce Back
Optimism characterizes the mood of Nevada executives this year. Whether it's waving goodbye to the recession or already having moved on to bigger and better things, business owners and executives have officially brushed off the oppressive economy and are now working on the bounce back. The 2014 Power Poll marks the twelfth year executives have weighed in on various issues … [Read more...]
NAIOP Southern Nevada Chapter Announces New Officers, Board of Directors, Committee Chairs and Liaisons for 2014
NAIOP Southern Nevada chapter, a trade organization representing commercial real estate developers, owners and related professionals in office, industrial, retail and mixed-use real estate serving the Southern Nevada market, has announced its 2014 officers, board of directors, committee chairs and liaisons. The 2014 NAIOP Southern Nevada officers are as … [Read more...]
Finding our Niche: Distribution Centers in Nevada
In an effort to diversify Nevada's economy away from over reliance on travel and tourism, hospitality and gaming, economic development authorities throughout the state continue to work to find the perfect industries for Nevada's business-friendly tax climate, wide-open spaces, multi-modal transportation systems and easy access to the 11 western states. In 2011, the … [Read more...]


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Epiq Systems Survey Reveals Key Gaps in Information Governance Strategies Among Major Corporations
London (5 Aug. 2014) – Epiq Systems, Inc. (NASDAQ: EPIQ), a leading global provider of managed technology for the legal profession, today announced its latest survey* findings, revealing the impact of growing data volumes on major corporations. More than three-quarters of the corporations surveyed feel confident in their ability to locate key data in the face of litigation or investigation. However, only around half of these companies continually monitor and update their data map, suggesting such confidence may be misplaced.
Epiq surveyed senior-level decision-makers within leading blue-chip businesses across four European regions – the U.K., Germany, Switzerland, and the Netherlands – to establish broad eDisclosure and document review trends within the corporate sector.
"The capture, storage and retrieval of data is a constantly evolving challenge, complicated by the sheer variety of connected and mobile devices that now generate data," said Martin Bonney, director, international consulting services, Epiq Systems. "Regulatory deadlines for document production can be as short as 14 days. If data is not continually assessed, the ability to respond to requests quickly, accurately and defensibly is severely tested."
Respondents also revealed three additional key data mapping challenges:
- 68 per cent of major corporations believe that growing data volumes, and the variety of devices on which that volume is now generated, cause challenges related to the safeguarding of information and data privacy
- 60 per cent of respondents report that growing data volumes generated by an increasing variety of devices make it difficult to identify where information is stored
- Increasing data volumes will lead to increased legal costs according to 56 per cent of respondents
Martin Bonney continued, "It is not enough to adopt an irregular pattern of data monitoring. Leading businesses are recognising the benefit of partnering with experts to adopt a pro-active, continual approach to information governance."
Further findings from the Epiq Systems survey will be released throughout 2014. To learn more about information governance strategies and data mapping, visit Epiq's resource center on the topic here: http://www.epiqsystems.co.uk/en-GB/Information-Governance/?LangType=2057
Survey conducted by telephone in November 2013, targeting 100 respondents from large "blue chip" companies (defined as having more than $500M annual revenues but with the majority in the study – 74 per cent - having more than $1B annual revenues) in UK, Germany, Netherlands and Switzerland. The largest European organisations in Manufacturing and Construction; Retail; Financial Services; Utilities; Pharmaceuticals; Professional Services and IT/Telecoms took part. Respondents were typically the CFO/Finance Director, Head of Compliance/Compliance Director or the Head of Legal/Legal Director/Head of Counsel.
About Epiq Systems
Epiq Systems is a leading global provider of technology-enabled solutions for electronic discovery, bankruptcy, and class action and mass tort administration. We offer full-service capabilities to support litigation, investigations, financial transactions, regulatory compliance and other legal matters. Our innovative technology and services, combined with deep subject-matter expertise, provide reliable solutions for the professionals we serve. Visit us online at www.epiqsystems.co.uk.
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The United Nations Independent Commission of Inquiry on Syria says that the Syrian government and rebels have each committed acts that go against international law, and they fear no repercussion. "The perpetrators of these violations and crimes, on all sides ... do not fear accountability," the commission's report says.
Full Story:BBC, Voice of America, Thomson Reuters Foundation, The Huffington Post


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Support Us
PCC Farmland Trust is a 501(c)(3) nonprofit land trust. Donations to the Trust are 100% tax-deductible, and we rely primarily on individuals to fund our farmland preservation efforts. To ensure that you, our donors and prospective donors, have full confidence in our organization and the causes we ask you to support, please read our Donor Bill of Rights.
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Conference Information
|Full Title:
|2nd International Conference on Dialect and Literature
|Short Title:
|Sheffield, United Kingdom
|Start Date:
|10-Jul-2013 - 12-Jul-2013
|Jane Hodson
|Meeting Email:
|click here to access email
|Meeting URL:
|Meeting Description:
|This conference is being held to coincide with the forthcoming 'South Yorkshire Voices' exhibition at Sheffield University Western Bank Library. The conference will open on the evening of Wednesday 10 July with a public poetry reading and discussion by Ian McMillan, and will close on the evening of Friday 12 July with a poetry workshop led by Peter and Ann Sansom, directors of The Poetry Business, which publishes The North poetry magazine. Our plenary academic speaker will be Dr Mark Sebba, who has written widely on pidgin and creole languages, and on the sociolinguistics of orthography.
|Linguistic Subfield:
|Ling & Literature
|LL Issue:
|Calls and Conferences main page


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Artificial Intelligence Firm Ampliphae
Enterprise, Trade and Investment Minister Jonathan Bell has announced Belfast-based technology company Ampliphae is to create 20 new high skilled jobs as part of... Find Out More
Fusion Antibodies Strike Green!
Pictured: Colin Walsh (Crescent Capital CEO) with Rick Buick, Paul Kerr and James Fair from Fusion Antibodies BELFAST, 5 Febuary 2016 – FUSION... Find Out More
Genesys snaps up SpeechStorm
BELFAST, 18 December 2015 – SpeechStorm has been acquired by the US Company Genesys. SpeechStorm was backed in a 2010 MBO from Kainos. This... Find Out More


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VIDEO: Boston Dynamics Shows Off Latest Robot 'Handle'By Angela Moscaritolo 02.27.2017Read More >
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The Best Website Builders of 2017By Michael Muchmore 02.27.2017Read More >
T-Mobile Wants to Turn Off 2G and 3G—But Not Quite YetBy Sascha Segan 02.27.2017Read More >
Mozilla Buys Bookmarking App PocketBy Angela Moscaritolo 02.27.2017Read More >
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Wahoo Fitness Tickr X Heart Rate Monitor
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The Best Desktop Computers of 2017By Joel Santo Domingo 02.27.2017Read More >
Apple Logic Pro X (for Mac)
Bottom Line: Apple Logic Pro X 10.3 is a powerful update to an already-excellent digital audio workstation, and if you own Logic Pro X, it's free.
Google Teases New Android Games, Play Store UpdatesBy Tom Brant 02.27.2017Read More >
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text: 360-Degree New York Stock Exchange Panorama Shows Where The Action, Sadness HappensJohn Mahoney10/24/08 11:00amFiled to: Atlas ShruggingPanoramaNysenew york stock exchangePhotography31EditPromoteShare to KinjaToggle Conversation toolsGo to permalink Computers have reduced tons of the bustle on the NYSE floor, but it's still a pretty charged place. Here you can duck the telescoping trading terminals as you swoop around a full 360-degree flash panorama courtesy of the NYTimes. Can you feel the pain? [NYTimes - Thanks, Dan!]Reply31 repliesLeave a reply


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Tropical Fish Keeping - Aquarium fish care and resources (http://www.tropicalfishkeeping.com/forum.php)
- Catfish (http://www.tropicalfishkeeping.com/catfish/)
- - Need Advice For Breeding Plecos (http://www.tropicalfishkeeping.com/catfish/need-advice-breeding-plecos-24418/)
Need Advice For Breeding Plecos
Hey all! I'm a bit new to breeding plecos, but I would really like to give it a shot! I just have a few questions for you guys!
- I'm getting 6 unsexed red marble long fin bristlenose Juvis (if I win the auction http://www.plecofanatics.com/forum/i...ew/frantic.gif) they are around 1-1 1/2 inches right now i believe. How old/big to they need to be before they can be sexed?
-How old/big do they need to be before they reach breeding maturity?
-What is a good tank set up for them?
-Can I have other fish with them, of is it best to leave them alone if Im planning to breed them?
-And is a 20gal long tank big enough for a breeding trio?
Thanks in advance guys!
yep- 20gallon should be ok to breed them in
they should be around 10-11cm (including fins) before you can see the males will have bristles
provide them with a cave and once they are sexually mature (around 1 -2 years old) they will be ready to breed. the cave should be only just large enough to allow the male to enter, and the male will grow quicker than the female generally.
they grow painfully slow compared to shortfins, so expect them to be quite small even when they are around a year old.
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Lyndon M STAFF said:There is no reason why that drive should not have worked, the connections are pretty much standardized across the board to accommodate the various brands of laptops. Doublecheck to be sure you have the drive placed absolutely correct on the bracket and try it again.
If it fails and the drive simply refuses to connect, I would suggest trying a Seagate brand.
BTW, thanks for the reminder- i need to get around to doing this myself with my 20 GB. :redface:
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Galaxy S3 Splash Screen then Nothing
I've tried searching and couldn't quite find this particular problem.. I apologize if I missed it.
Phone: Sprint Galaxy S3
Version: stock 4.3 (been running this for quite a while with no issues)
- As soon as I put in the battery I get a single vibrate and the phone tries to boot. It shows the Samsung splash screen then goes blank. Not sure if it's still running (with screen blank) or has shut off, or whatever.
- The phone is trying to boot immediately after putting in the battery. I don't have to hit the power button.
- If I hit the buttons for recovery mode right after putting in the battery I see the message at the top about recovery mode but as above, I never see the recover screen... the phone goes "blank."
- Same thing trying to enter download mode. I can briefly show the download screen then everything goes blank.
- I've tried a different battery, booting with several different power cords, etc.
Now the really odd part... after repeatedly trying I did manage to get the recovery mode to start. I wiped the cache and the phone seemed to reboot normally. However, after trying a shutdown and restart it started the same pattern above with stopping after the splash screen. So far haven't been able to get back into recovery mode.
Any help is appreciated. I hope there is an option other than a bricked phone.
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Biz2Credit said while capital can still be a challenge for small businesses to come by, financing from credit unions and small, regional banks is on the rise.
Eleven states and the District of Columbia split $360 million in State Small Business Credit Initiative funding.
More entrepreneurs are expressing their irritation with the credit union member business lending cap
The Small Business Administration has announced a new fund that will focus exclusively on providing capital to Michigan businesses.
Since March 2010, Ohio's credit unions have boasted a $23.7 million gain in member business loans.
Amid reports of sluggish job creation, some credit unions are still helping small businesses grow.
North Side Community Federal Credit lends to job-creating couple through the city's Small Business Development Fund.
League president says four Missouri CUs are at or near their cap, says raising cap to 27.5% would generate 1,676 jobs in his state.
With only a handful of credit unions enrolled, the state of California is pulling out all the stops to get more signed on to a nearly $100 million business lending program.
Centris FCU feted for aggressive lending to small businesses, including 24 loans in 2010.


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Regional North America United States Minnesota Government Federal US House of Representatives Collin Peterson [D-7]
Related categories 1
Sites 18
Congressman Collin Peterson
District 7, Minnesota. Democrat. Press releases and op-ed pieces, FAQ for constituents, contacts. Information on the District, a huge, largely rural, swath of western Minnesota.
Ballotpedia - Collin Peterson
Wiki about the Minnesota politician, from a libertarian, free-market perspective.
Biographical Directory of the United States Congress - Collin Clark Peterson
Short biography along with political party, years of service in Congress and bibliography.
Bloomberg - Collin Peterson
Collected news articles, videos, photos and blog posts about the Minnesota politician.
C-SPAN Video Library - Collin Peterson
Videos and transcripts of appearances on C-SPAN.
Congress.gov - Collin C. Peterson
Information on sponsored and cosponsored legislation. From the Library of Congress.
FEC - Candidate Summary Reports: Collin Clark Peterson
Official campaign finance reports and data from the Federal Election Commission.
GovTrack.us - Rep. Collin Peterson [D-MN7]
Legislative profile and bill tracking.
IMDb - Collin Peterson
Lists of film and television appearances along with related videos.
Minnesota Legislative Reference Library - Collin Clark Peterson
Detailed political profile including information about each session served.
NNDB - Collin Peterson
Formatted profile of the Minnesota politician.
On the Issues - Collin Peterson
Provides quotes and background on various international, domestic, economic and social issues. Includes profile, contact data, forum and voter match.
OpenSecrets.org - Collin Peterson
Career profile of campaign funds raised and spent.
Project Vote Smart - Collin Peterson
Provides a biography, committee assignments, campaign finances, voting record, issue positions, interest group ratings, speeches and public statements.
Roll Call - Rep. Collin C. Peterson (D-Minnesota)
Profile, election history and collected news reports.
Washington Post - The US Congress Votes Database: Collin Peterson
Features complete voting record along with a brief biography, roles in Congress and financial disclosure statement.
Wikipedia - Collin Peterson
Crowd-sourced encyclopedia article about the Minnesota politician.
WorldCat Identities - Collin C. Peterson 1944- (Collin Clark)
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1st Month FREE! 1st Month FREE! Welcome to Maple Gardens, an owner managed, No Fee, gated rental apartment community in Irvington, New Jersey. Maple gardens is located Only 30 Minutes From Mid-Town tan. Our No Rental Apartments start at just $650 per month, and we offer a level of secure → Read More
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The Editors of the Vegetarian Times bring you their favorites in Vegetarian Times Fast and Easy: Great Food You Can Make in Minutes, including recipes for Cheese Beignets; Edamame Succotash; and Tempeh Triangles with Piccata Sauce.
Serves 8
Beignets are commonly referred to as the "Southerner's doughnut." In this family recipe, the dough should be stiff—add just enough water so that you can press the dough flat. To make cutting the stiff dough easier, use a floured pizza cutter.
1 Stir together flour, sage, baking powder and salt in medium-sized bowl.
2 Whisk together eggs, olive oil and hot sauce in large bowl. Gradually stir in flour mixture. Add 3/4 cup water a little at a time, combining well until dough is thick. Fold in cheese.
3 Press dough into 1/2-inch-thick rectangle on well-floured work surface. Cut into 1-1/2-inch squares.
4 Pour vegetable oil into large skillet to depth of 1/2 inch, and heat over medium heat until small piece of dough sizzles gently in oil. (The dough should not brown immediately.)
5 Place beignet squares in oil, taking care not to overcrowd (you may need to fry in batches). Fry 3 minutes on each side, or until browned and done throughout. Remove with a slotted spoon to paper towels to drain. Sprinkle immediately with coarse salt. Right before serving, dust with cayenne.
Per Serving, 190 cal; 7g prot; 7g total fat (1.5g sat. fat); 25g carb; 55mg chol; 330mg sod; <1g fiber; 19 sugars
Buy Vegetarian Times Fast and Easy
This page created June 2008
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So it's like a Replica collection of a Replica collection?! META
post #65326 of 109053
10/11/12 at 5:46pm
Hidden arms is the new hidden placket
How do people find anything on Yoox?
The h&m+mmm sneakers look terrible, but the boots are pretty sick. There´s your footwear upgrade, kindofyoung.
What do you guys think the price will be like??
On another note, I picked up a package from the post office and didn´t have to pay anything to fucking customs, finally.


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Don Cheadle Net Worth: Don Cheadle is an American actor who has a net worth of $35 million. Born November 29, 1964 in Kansas City, Missouri, Don Cheadle entered the California Institute for the Arts (CalArts) in Valencia, California in 1982 where he received a rigorous education in classic theatre, after which he quickly found work in television, appearing in small parts on shows such as Fame (1982-87), L.A. Law (1986-94) and Hill Street Blues (1981-97). He had a role in Picket Fences and followed it with performances in Devil in a Blue Dress (1995), Rosewood (1997) and Boogie Nights (1997). Soon after, he began collaborating with director Steven Soderbergh and appeared in the movies Out of Sight (1998), Traffic (2000) and Ocean's Eleven (2001). Other Cheadle films include The Rat Pack (1998), Things Behind the Sun (2001), Swordfish (2001), Crash (2004), Ocean's Twelve (2004), Ocean's Thirteen (2007), Reign Over Me (2007), Talk to Me (2007), Traitor (2008), Iron Man 2 (2010) and Iron Man 3 (2013). In 2004, his lead role as Rwandan hotel manager Paul Rusesabagina in the genocide drama film Hotel Rwanda earned him a nomination for an Academy Award for Best Actor. As a result of his philanthropic efforts following the movie, in 2007 Cheadle was awarded the BET Humanitarian award of the year for his numerous humanitarian services rendered for the cause of the people of Darfur and Rwanda. On December 13, 2007, Cheadle and fellow actor George Clooney were presented with the Summit Peace Award by the Nobel Peace Prize Laureates in Rome for their work to stop the genocide and relieve the suffering of the people of Darfur. Cheadle was cast as Marty Kaan on the Showtime sitcom House of Lies, for which he won a Golden Globe Award in 2012 and was nominated again for the series in 2013 and 2014.
Don Cheadle Net Worth
Don Cheadle
Date of Birth
Nov 29, 1964 (52 years old)
Actor, Film Producer, Television producer, Author, Voice Actor, Film director, Screenwriter
United States of America
5 ft 8 in (1.734 m)
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Don Cheadle Earnings
- Iron Man 2$1,000,000
All net worths are calculated by applying a proprietary algorithm. The results are then fact checked and confirmed by a team of editors. We scan all publicly available data and resources and work diligently to ensure that our numbers are the most accurate and up to date net worth data you will find anywhere on the internet.


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Jessica Simpson can't wait for her wedding. Talking with ET correspondent Lance Bass at Forbes' Women's Summit: Power Redefined in New York City on Thursday (May 15), the fashion mogul opened up about her past reality career and her upcoming wedding to … [Read more...]
Jessica Simpson's Diaper Buddies
Like Brother, Like Sister! Jessica Simpson took to her Instagram to share a sweet shot of her children Maxwell and Ace on Tuesday (May 6). "Diaper buddies," the proud mom captioned the shot of the siblings sitting in there diapers next to one another. … [Read more...]
Jessica Simpson Shares a Sweet Shot With her Little Angel
Too cute! Jessica Simpson took to Instagram to share a sweet shot of her almost 2-year-old daughter, Maxwell, sharing a mommy-daughter kiss. "Kissed by an angel... for a SNICKERS egg ;)" the singer captioned the shot on Friday (April 18). … [Read more...]
Jessica Simpson Shares Sweet Daddy-Daughter Photo
Daddy and his little girl! Jessica Simpson to0o to Instagram to post two sweet shots of fiance Eric Johnson bonding with their almost 2-year-old daughter Maxwell at the beach. "Maxi and Daddy at the beach. Yikes and Yay. Good Lord! She has NO fear!" the singer … [Read more...]
Jessica Simpson's Daughter Maxwell Stops to Smell The Roses
Jessica Simpson took to her Instagram to share a shot of her growing daughter, Maxwell, enjoying a day in the sun on Monday (April 8). "Stop and smell the roses Maxi... Don't eat them!!!#rosepetaltongue," the mom of two captioned the shot of the 23-month-old picking roses in … [Read more...]
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He said, 'Christy, can I talk to you about this moment.'
Ex-Terp Christy Winters-Scott talks good game for Mystics →
"Thank you for having me," said an appreciative Wilbon looking directly at Mitchell. "It's been a few years since I've been able to be here. I really do appreciate the invitation."
Hall of Famer Bobby Mitchell tackles leukemia once again with golf tourney →
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- //Millencolin
Swedish punkers Millencolin made quite the breakthrough with their last record, Pennybridge Pioneers. On their first three records, changes to their sound were hardly noticeable, as they plowed through song after song of speedy, melodic, sometimes ska-inflected skate-punk. They were pretty damn good at what they did, but there's really only so long that a band can mine that style before they either break up or feel the need to diversify. Pennybridge found Millencolin realizing that they were not only a great skate-punk band, they were a great band, period. They slowed the tempos down a notch, and started to emphasize songcraft over velocity. The end result was one of the best rock records of Y2K. Seemingly taking a hint from their contrymen Fireside and Starmarket, who specialize in heavy, melodic, emotional rock of the highest order, Pennybridge Pioneers managed to be smart, tuneful, and addictive, all the while rocking your pants off.
So, the question that rears its head with the band's new release, Home From Home, is, of course, will the band continue their winning streak, or simply relapse into old habits? Pennybridge was such a self-confident stylistic departure that it's pretty tough to imagine the boys simply lapsing back into the speedy punk rock of old. And while they certainly don't do that on Home From Home, they unfortunately don't quite keep up the bar that they set with Pennybridge. The best songs on the record pretty much go for the midtempo, melodic stuff that the band perfected on Pennybridge. "Montego" soars on a fantastic chorus that recalls the glory days of Bob Mould's Sugar, and "Black Eye" features some great guitar interplay, as well as a great shout-along chorus. "Botanic Mistress" sees the band at it's happy, pogo-inducing best, with another fun shout-along chorus that goes "All I wanna do / Is live my life with you". If I had to guess, I'd say that this song is an ode to pot, but it's hard to say because the cheap bastards at Epitaph saw fit to only send me a cheesy advance copy of the CD in a cardboard case with no lyrics. This is significant in that singer Nikola Sarkevic has the tendency to actually write some pretty damn good lyrics. While sometimes they're fluffy and more than a little bit silly, he has the tendency to throw in some startlingly insightful lines here and there. While these little lines have the tendency to get lost in the bluster of the band's music and in Sarkevic's slight Swedish accent, the inclusion of a lyric sheet is always welcome. However, no dice on that front, so unfortunately, a lot of the subtleties of Sarkevic's thoughts will have to be left to head scratching punk-rockers listening intently with their headphones.
Home from Home
US: 12 Mar 2002
UK: 11 Mar 2002
Although there are quite a few highlights on Home From Home, the record as a whole is not nearly as cohesive as Pennybridge was. For one thing, it starts off on the wrong foot with "Man or Mouse" with its unfortunately boneheaded "yeah, yeah, yeah, yeah" chorus. From there, the band launches into three strong cuts: the speedy, melodic "Fingers Crossed" and the above-mentioned "Black Eye" and "Montego". The next two tunes mark the low point of the record—"Punk Rock Rebel", which is supposedly an affectionate tribute from the band to one of their early skating heroes, comes across as cheesy and forced, while the metal-tinged "Kemp" is just tuneless and annoying.
The second half of the record is pretty damn watertight, but even so, there's nothing here that quite catches fire like Pennybridge's standout tracks. "Afghan" is a thoughtful meditation on the war on terrorism, and with lyrics like "So show the world you're strong/The weaker one is wrong", delivered with his trademark bellowing sneer, it's clear where Sarkevic's sentiments lie. Lines like these show that while Sarkevic may not be the most adept wordsmith the pop music world has ever known, he does have some interesting things to say, and he manages to convey them efficiently and unpretentiously.
Home From Home is not a bad album. In fact, it's actually pretty damn good. Sarkevic is still one of the best singers in all of punk rock, and the band pretty much mops the floor with their peers when it comes to songwriting prowess and general musical excellence. They happened to pull off quite the stunner of a record with Pennybridge Pioneers, and Home From Home's main fault is that it doesn't quite hold up when compared with that high-water mark. However, judged by any other standard, and compared to practically any other modern pop-punk record released this year, Home From Home holds its own with no trouble whatsoever.
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Depeche Mode "Where's the Revolution" (Singles Going Steady) // Music
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Esther having had power with God, and prevailing, like Jacob, had power with men too. He that will lose his life for God, shall save it, or find it in a better life. The king encouraged her. Let us from this be encouraged to pray always to our God, and not to faint. Esther came to a proud, imperious man; but we come to the God of love and grace. She was not called, but we are; the Spirit says, Come, and the Bride says, Come. She had a law against her, we have a promise, many a
promise, in favour of us; Ask, and it shall be given you. She had no friend to go with her, or to plead for her; on the contrary, he that was then the king's favourite, was her enemy; but we have an Advocate with the Father, in whom he is well pleased. Let us therefore come boldly to the throne of grace. God put it into Esther's heart to delay her petition a day longer; she knew not, but God did, what was to happen in that very night. (Es 5:9-14)
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Haman prepares to hang Mordecai.
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Esselte Ready-Tab Hanging File FolderColor Hanging Folders
Letter - 1/5 Tab Cut - 25/ Box - Blue - View Complete Details
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Made in America
This product was manufactured in the United States.
Esselte Ready-Tab Hanging File Folder - Ready-Tab Reinforced Hanging File Folders feature lift-tab technology that makes it easier than ever to tab hanging folders so your filing system is more efficient. Easily change tab position with tabs that are built into the folder. Tabs easily lift up and down. Write directly on tab or use labels. Printer-ready sheet of labels are included, and labels have permanent self-adhesive. Folders feature polylaminate strips on top and bottom edges for superior durability.
- Preattached clear tabs mean no more lost tabs or guessing where to position them.
- Easy instructions for quick tab assembly.
- Includes letter-size, printer-ready inserts.
- Wear points are polylaminate reinforced.
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The Crossroads of the Aether
74 members39 Comments 16 Likes
487 members187 Comments 13 Likes
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Lord Rex of Whitby has not received any gifts yet
Thank you for your request - you're too kind. I really should update my photos; they're all quite out of date. Unfortunately, I tend to look rawther the same in all of them...
© 2017 Created by Hephzibah Marsh.


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Top 14 most nutritious fruits
Fruit can be expensive so it makes sense to buy the cheapest you can find.
Fruit can be expensive, so it makes sense to buy the healthiest you can find.
The following varieties have the most nutrition for their cost, are low in sugar and toxic pesticides, and have plenty of vitamin C, fibre, folate and potassium:
- apricot
- avocado
- banana
- grapefruit
- honeydew melon
- kiwifruit
- orange,
- nectarine
- papaya
- pear
- rock melon
- star fruit
- tangerine
- watermelon
Source: Environmental Working Group.
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This model was realized using 3D Studio Max (version 2011) and Vray rendering engine (version 2.0), so it is optimized for working in this environment.
We can not be held responsible for the use of this model in environments different from the recommended one.
The model is a high poly composed by quads and triangles distributed across the topology in a well balanced way.
The rendering shown was made using Vray materials and scene setup provided in the file MAX (Vray lights, Vray materials and environment for scene setup).
TurboSmooth modifier was applied to the model for achieving the best quality.
The renderer visible in the scene can be obtained just using the native file (.max).
Textures are from 2048×2048 to 4096×4096.
All textures are in TGA format.The package includes three versions of team kit:
- Green;
- Orange;
- Grey;
FEATURES:The package includes two versions of the file:
- Default scanline version with standard materials and lights;
- VRay version with vray materials and VrayLights;
The model is rigged using the standard 3dsmax biped, with the addition of a bone for both the eyes, managed by a "look at costraint" to allow its rotation.
Facial expressions are handled with the morpher modifier.The scene includes five facial morphs:
- Angry
- Left blink
- Right blink
- Teeth-grinding
- Serious
The package includes 6 animations:
The file .obj contains only the geometry (T-pose and heads with static facial expression). So any facial morphing, rigging and animations must be created from the scratch by the customers.
The FBX format included in the package can be used as it is provided, and is not editable. To get a similar quality of rendering images of the gallery, you must set the materials, lights and photo set independently.
For any questions or doubt please feel free to contact us.Please don't forget to vote the model. We'll really appreciate it. Thank you!


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Are you looking for Service Station near MANDURAH? Hotfrog helps you find the most popular businesses related to Service Station near MANDURAH WA! Find the best Service Station in MANDURAH.
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1745 Crowberry Drive,Dallas TX, 75228
Sold Price
3 beds , 3 Full baths
1,668 sq. ft
Status: Sold
Berkshire Hathaway HomeServices PenFed Realty Texas
(214) 901-7993
Last Updated: 2/26/2017 9:29 PM
Description for 1745 Crowberry Drive Dallas, TX 75228
Exciting value for your money in this 3-BR, 3-BA mid-century modern home. Home has been well-maintained and features an open concept kitchen_den with marble tile counters as well as soothing gray ceramic floor tile. The large hall bathroom has been updated with gorgeous granite counters. Original hardwood floors throughout add a wonderful sense of history and warmth. Only minutes from White Rock Lake, Casa Linda shopping, and the Arboretum.
Listing Information
Lake Park Estates 6
City Sewer
School Information
Room Information
Interior Features
Gas Range/Cooktop, Refrigerator
Ceramic Tile, Wood
Central Air
1, Brick, Gas Starter
Walls - Panelling, Wainscoting
Exterior / Lot Features
2 Garage Spaces, 2 Parking Spaces, Garage
Less Than .5 Acre (not Zero)
Financial Considerations
Inside AccessTM
Current market conditions for 1745 Crowberry Drive
Active Market Trends
Source:MLS DATA
Median list price$435,000
Days on market80
Properties for sale2,408
Sold Market Trends
Source:MLS DATA
Median sold price
Not enough information to display market trend data at this time
Sales to list price
Not enough information to display market trend data at this time
Properties sold2,430
Median sq ft price$-1
Arrows indicate 90 day trend
Listing Price History for 1745 Crowberry Drive
Dallas Real Estate
Dallas is located in Texas. Dallas, Texas 75228 has a population of 1,207,202.
The median household income in Dallas, Texas 75228 is $42,436. The median household income for the surrounding county is $49,159 compared to the national median of $53,046. The median age of people living in Dallas 75228 is 31.8 years.
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