text:
LAKE FOREST, Ill. -- The Chicago Bears officially ruled out receiver Earl Bennett on Thursday for their matchup against the Minnesota Vikings on Sunday.
Bears coach Lovie Smith said Bennett "is making progress," but won't be ready to play by Sunday after suffering a concussion against Seattle, making him the second player the club officially ruled out. The Bears announced earlier in the week that linebacker Brian Urlacher, who attended Thursday's practice, would miss Sunday's game as he recovers from a hamstring injury.
"Earl Bennett is not playing this week," said Smith after the workout inside the Walter Payton Center. "We have two guys out."
In other injury news, backup running back Michael Bush (ribs) sat out his second consecutive practice, along with Tim Jennings (shoulder), who Smith said hasn't yet been ruled out for the matchup against the Vikings although it's unlikely he'll play.
"Michael Bush is making progress, but he didn't practice today. Tim Jennings is getting better, also," Smith said. "The rest of the guys all practiced on a limited basis."
After returning to the practice Wednesday to work in a limited capacity, receiver Devin Hester missed the portion of Thursday's workout open to the media as he was being evaluated by doctors, but returned later to participate on a limited basis, which likely means he hasn't yet been cleared for full contact.
The Bears also listed guard/tackle Gabe Carimi (hamstring) as participating in a limited capacity along with Chris Spencer (knee) and defensive tackle Stephen Paea, who missed Wednesday's practice but returned Thursday for a limited workout.
Rookie Alshon Jeffery (knee) was limited in his second consecutive practice, but the Bears expect him to return to the lineup Sunday after he sat out of the last two games recovering from a Nov. 21 arthroscopic knee surgery.
Jeffery missed five games with a fractured right hand prior to suffering the knee injury Nov. 19 at San Francisco that resulted in him sitting out the last two contests.
Spencer's status, meanwhile, remains uncertain. A source said the club is optimistic Spencer will be available to play against the Vikings, but the club might still opt to start Edwin Williams opposite Gabe Carimi at guard.
Defensive end Jared Allen (shoulder/back) and running back Adrian Peterson (coach's decision) sat out of practice for the Vikings. Tight end Kyle Rudolph (shoulder), linebacker Jasper Brinkley (shoulder), offensive tackle Phil Loadholt (ribs/knee) and cornerback Antoine Winfield (knee) were limited.
The Vikings listed punter Chris Kluwe (knee) and defensive tackle Letroy Guion (shin) as participating fully.


text:
Ongoing work for 3D Product Modelling
- Status Closed
- Budget $8 USD / hour
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Project Description
I have ongoing work related to our previous project '3D Product Modelling'Get free quotes for a project like this
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subset: commoncrawl


text:
The Devil's Backbone (2001)
Plot Summary
It is 1939, the end of three years of bloody civil war in Spain, and General Franco's right-wing Nationalists are poised to defeat the left-wing Republican forces. A ten-year-old boy named Carlos, the son of a fallen Republican war hero, is left by his tutor in an orphanage in the middle of nowhere. The orphanage is run by a curt but considerate headmistress named Carmen and a kindly Professor Casares, both of whom are sympathetic to the doomed Republican cause. Despite their concern for him, and his gradual triumph over the usual schoolhouse bully, Carlos never feels completely comfortable in his new environment. First of all, there was that initial encounter with the orphanage's nasty caretaker, Jacinto, who reacts even more violently when anyone is caught looking around a particular storage room the one with the deep well. Second, and more inexplicable, is the presence of a ghost, one of the former occupants of the orphanage named Santi. Not long after Carlos' arrival, Santi latches onto Carlos, badgering him incessantly at night and gloomily intoning, "Many of you will die." As if that wasn't enough to keep the orphanage's occupants in an unrelenting state of terror, there's the un-exploded bomb that dominates the orphanage's courtyard, still ticking away; With the orphanage left defenseless by its isolation, and the swift progression of Franco's troops, the ghost's prediction seems depressingly accurate. Nevertheless, with every step of the plot, it becomes apparent that the ghost's predictions as to who (or what) will die, the real source of danger and even the definition of death itself may be more ambiguous than first thought.- Written by Anonymous
During the Spanish Civil War, the twelve year-old boy Carlos is left behind by his family in an orphanage in the middle of nowhere. Dr. Casares and his wife Carmen run the orphanage and the former orphan Jacinto, who lives with the teacher Conchita, is responsible to take care and for the maintenance of the place. Jacinto is an ambitious and violent man and has a love affair with Carmen trying to steal the key of the safe where Dr. Casares and his wife hide the gold that is used to support the Republican forces. Carlos is bullied by the strong boy Jaime, who is the leader of the other boys. One day, Carlos feels the presence of a ghost and soon he learns that he is the orphan Santi that is missing. Carlos sees Santi several times and one day he learns the dark secret of his disappearance.- Written by Claudio Carvalho, Rio de Janeiro, Brazil
After Carlos - a 12-year-old whose father has died in the Spanish Civil War - arrives at an ominous boys' orphanage, he discovers the school is haunted and has many dark secrets that he must uncover.- Written by Daniel Jos. Leary
After his father dies in combat during the last months of the Spanish Civil War, young Carlos (Fernando Tielve) is brought to an isolated orphanage in 1939...See full synopsis » (Warning: contains spoilers!)


text:
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ZEX™ Quick Tech: ZEX™ Safety Blow Down Kit Install
This Quick Tech Video helps users understand how to easily install the ZEX™ Safety Blow Down Kit whether their Nitrous bottle's valve features an internally or externally threaded safety port. The installation process is explained through a series of helpful demonstrations and animations utilizing all of the kit's parts and pieces.
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text:
I couldn't agree more, Marge. What I can't understand is why, when I have
for years, determinedly pulled garlic mustrad out by the bushel as soon as it
appeared in the spring, before it bloomed, I keep getting masses of it. I guess
the seed must live in the soil for years. If it were just brought in by
birds, there couldn't possibly be so much of it. Birds bring in lots of the
Oriental bittersweet, and I find that seeded just about everywhere, but not in the
msses that garlic mustard makes.
In a message dated 01/21/2004 1:06:43 AM Eastern Standard Time,
firstname.lastname@example.com writes:
> I find it has a faint scent of garlic when handled a lot that sort of
> envelopes you and by the time you've pulled 4 or 5 wheelbarrows full,
> you're ready to gag, and I actually like garlic; use a lot of it.
> But, garlic mustard's garlic scent is somehow also cloying...hard to
> explain; guess you hafta pull a few million to understand what I
> mean:-)
Support hort.net -- join the hort.net fund drive!


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Check Out the new Remax App
Ask About This Home
Well built and well maintained 4th row cottage in Whalehead! For slightly more than the cost of a lot, you can own your own place at the beach. Four bedrooms, 2 full bathrooms, an open greatroom and well equipped kitchen that all come fully furnished are just waiting for your personal touches. Low maintenance vinyl siding and a dry ground floor entry add to the appeal. Enjoy it as it is, or add more living space and a pool on the large 20,000 sq. ft. lot. Or have the house moved to a less expensive lot and build your dream home. Several options are possible for this great opportunity! Priced to sell.


text:
Website Design
This project received 53 bids from talented freelancers with an average bid price of $485 USD.Get free quotes for a project like this
Project Budget$250 - $750 USD
Total Bids53
Project Description
To re-create my website ([url removed, login to view] hosted on [url removed, login to view]) so that it can be viewed on any device (no Adobe Flash). I want the finished file to be installed on my son's server. I also want the ability to make minor changes from time-to-time to photos or text.
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News Aggregator Wordpress theme with the look and feel of pinterest
- Status Closed
- Budget $10 - $30 USD
- Total Bids 5
Project Description
I need News Aggregator Wordpress theme e.g [url removed, login to view] with the look and feel of pinterest.com. , could be like [url removed, login to view] but with features of onenews.
note1: I have purchased ONE LICENCE of onenews theme.
Popurls is the ultimate source for the latest web buzz and a daily must-read for me. I thought I can work out something similar to keep track of the buzz on WordPress and thus OneNews was born. With this news aggregator theme, you can even create unlimited pages of news like Alltop.
Now you can have
unlimited pages with your targeted keywords
self-updating fresh content (no maintenance time required)
Key Features of OneNews
2 color schemes – Light & Dark
Responsive design
Easily create a single-page news aggregator like Popurls or multiple-pages news aggregator like Alltop
Widgets Ready Homepage – easily add and arrange feed blocks without touching any codes
4 unique widgets available
- textRSS: for normal text feeds
- photoRSS: for Flickr feeds
- videoRSS: for Youtube, Vimeo, Metacafe and Dailymotion feeds
- HTML: for any php or html code, including javascript (easily add banners or adsense)
Inline Video Player for Youtube, Vimeo, Metacafe and Dailymotion
Tooltip with thumbnail display, social media icons, new item indicator, relative date
Option to open link in new or current window or iframe
Option for dofollow or nofollow feed links
Optional 4-column widget-ready bottombar
Display blog as homepage or as a sideblog
Threaded & paged comments with Gravatar
Robust SimplePie RSS with built-in cache
No plugins required
Social media ready
Localization ready
Lifetime upgrades
note2: I will add some new features in the future.Get free quotes for a project like this
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Medically Reviewed on November 6, 2014 by Healthline Medical Team
Pancreatic vein
Located in the pancreas, the pancreatic vein is responsible for draining the pancreas into the superior mesenteric and splenic veins.
It contributes to the significant role of the pancreas since this gland has a diverse set of functions in digestion and sugar metabolism. The pancreas also serves as a storage reservoir for several digestive enzymes.
The pancreatic vein is connected to several small veins that drain the body and tail sections of the pancreas.
Written and medically reviewed by the Healthline Editorial Team
Co-developed by:
In Depth: Pancreatic vein
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16 Bridge Gardens, East Molesey, KT8 9HU
Hi, I'm Clare. I'm a mum and photographer. I specialise in lifestyle photography and my style is natural and authentic - I love to capture love, laughter and connection.
I photograph families in places that are meaningful to them - either their home or a beautiful outdoor location. No stark white backgrounds, or studio lights - I want each family I work with to walk away with a set of photos that capture the essence of who they are, and that they will treasure forever.
I cover all of South West London and Surrey, and offer newborn, maternity and family photography sessions. You can read more about what I do, and see examples of my work on my website: www.claremurthy.com. You can also find me on Facebook at www.facebook.com/claremurthyphotography where I share recent work and tips for taking better photos of your children.
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It's 2016. You know what this means, right? (Other than time is clearly speeding up.) It's another insane/stressful/chaotic/divisive election year—which, aside from the whole "democracy is awesome and we should all be participatory citizens" thing, is pretty much the worst thing ever. Especially if you're a thinking Christian.
Every year, as magazines are wont to do, we like to poll RELEVANT readers. We ask how we're doing, and what they want to see in the magazine. Then we sneak in my favorite question: "What do you think of RELEVANT's political content?" Read More


text:
This Donna Howell-Sickles Greeting Card Assortment has been discontinued. Order today to take advantage of incredible Warehouse Clearance Pricing!
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We guarantee your complete satisfaction! If for any reason you are not completely
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text:
The National Health Care Day of Service is today, and volunteers across the country are taking place in thousands of events designed to raise visibility for health care reform in their communities. These health care service projects give volunteers a chance to make an impact in their own communities and improve the lives of their neighbors in the short term -- and they are just one step forward in the larger fight for long term, comprehensive health care reform.
Shonda from Greensboro, North Carolina, is making an impact on her community. Her National Health Care Day of Service event this Sunday will provide materials to local parents about State Children's Health Insurance Program (SCHIP). She is expecting about 70 people to attend, including families she met at schools, churches, day cares and summer camps.
The Family Fair is being held at the Greensboro Public Library. The focus of the fair is providing information and applications for SCHIP to families in need. There will be tables with SCHIP information and applications, as well as food and entertainment for the kids. Two guest speakers, whose families are enrolled in SCHIP, are set to speak at the fair. Shonda has even arranged for a Spanish translator. Despite her busy schedule, Shonda is passionate about the impact of her community service.
"We are really trying to make a difference and help out as much as we can," Shonda explained. "Even with our personal lives and schedules, we find ways to make it work."
Meanwhile Pat from Chesapeake, Virginia, is planning a similar event. This Saturday, Pat will provide SCHIP information and applications to customers at local businesses, such as shopping malls and grocery stores. He is expecting a diverse crowd of around 30 volunteers to help him. The group will include health care professionals, educators, and people who are struggling with their own health care.
These are two of the many of events taking place today, and with more events scheduled tomorrow as well. There may still be time to find a service event in your area this weekend. If you attended an event, let us know how it went in the comments below.


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text:
"Pinterics, Michael W (MED)" wrote:
> Ah Kris... give up Fargo? for chicago?
Yes, hard to believe, isn't it? Winter is still roaring here, while I hear
from my cousin that there are such things as pussy willows appearing in the
Chicago area. Someone here told us that the polar bears are starting to move
south from Churchhill, Manitoba due to global warming. So that about did it!
Polar bears! And Churchill is about a thousand miles to the north of us.
> Good luck, I would have to believe that there will be more
> opps for hosta than you've currently seen in ND. I could
> be wrong.
I am from South Bend, so I know that at least the growing season is longer. I
don't plan to move my large hosta collection (well, maybe a few choice minis),
but will start over; this will be a lot of fun, I think.
Ubi sunt qui ante nos fuerunt,
Kristi A. Groberg, Ph.D.
Fargo, ND 58103
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text:
After a couple years of rubbing shoulders with the "gear review" world, I've come to realize a couple things:
- Gear reviews are frequently biased because the reviewer is getting free product.
- They often don't show a product's true capabilities–table top reviews, etc.
With this in mind, I've made the following changes to The Daily Prep's gear review policy:
- I will probably say bad things about your product. This doesn't mean I will invent things and be mean, but I will not shy down from pointing out things I see that could be improved upon.
- If I agree to review something, I will use it for it's intended use (or as close to it as absolutely possible). If I like your product, we can brainstorm my review together. I'm not against trying to go hard and be extreme!
- I will return any and all products sent to me for review after I am done using and reviewing. Manufacturers are free to re-issue to somebody else for another review, take back into inventory and sell, or donate it to another worthy cause of their choosing.
If this doesn't deter you, send me a note. I'd love to check out anything you think can help us take steps towards preparedness.


text:
The Hotel at Holmes has 78 rooms and suites, including three executive suites. For additional information and reservations, please call (815) 753-1444. You may also request a room reservation online.
Available Rooms Pictured Below
DeKalb, Illinois 60115 | Regional Sites | Contact Us Emergency Information | Employment | Maps | Accessibility
© 2017 Board of Trustees of Northern Illinois University. All rights reserved. Web Site Privacy Policy


text:
Get beautiful custom wedding cakes in the Belleville, Illinois area!
(618) 654-8180
If you are looking for a true artist to make your cake stand out from all the other wedding cakes, you have come to the right spot! Patty Cakes is a family owned and operated business that creates beautiful custom cakes for the Belleville, Illinois. We've been in business since 1981 and now span two generations. We've been featured on TLC's "Ultimate Cake-Off," and have baked cakes for all types of people across the Midwest Area.
We're conveniently located near Belleville, and invite you to call us with any questions you have; we're more than happy to answer them. Talk to us about the range of things we can make for you, and find out how we can help you make your next celebration special. You can also find us online for more details about us, or to make ordering easy!
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All cakes are made with fresh ingredients. You are invited to set up a tasting before you order your wedding cake. We suggest that you try to have your wedding cake ordered 6 months to a year in advance if you have the ability. What makes our cakes extra special is that we don't use the wooden dowels to support our cakes like the majority of other bakeries. We have stainless steel cake supports to make sure your cake doesn't fall. Choose from 12 flavors and 8 fillings to create the perfect flavor combination. If you have found a picture of a wedding cake that you would like us to recreate, don't hesitate to bring it in.
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text:
Bucharest In Your Pocket's first visit to the huge Bucharest market at Obor would have been some time early in the spring of 1999, just as we were preparing our first guide. We were looking for one of those little metal devices you shoved into your television cable in order to get free HBO. In those days that's the kind of place Obor was: a byword for dodgy deals. The old market hall where you bought your meat was accompanied by an open-sided building where costermongers sold fruit and vegetables, while the surrounding area was a kind of shanty-town where traders sold just about everything, and where you didn't ask any questions about where the goods came from.
Obor has been a market since at least the 18th century, when it was known as the Piata Targului de Afara, a place for traders from outside of Bucharest to do business; the name Obor came into use during the early part of the 19th century. During this period Obor was infamous as the location of Bucharest's gallows: public executions would take place on market days to ensure a good crowd.
The practice was halted in 1823, but the gallows itself remained in place until 1870. In 1877, market traders raised a stone cross on the site of the gallows to commemorate those killed here. The cross, despite being declared a protected monument in 2004, was nevertheless moved in 2009 during the renovation of the market.


text:
Use the 5.11 Flashlight Belt Clip/Holster to conveniently attach any 5.11 ATAC model of flashlight to you or your kit. Made from high density impact resistant polymer and can be installed or removed in just a few seconds. Compact and versatile, it will fit on a wide variety of belts and takes up just 1.1" of space.
Additional Information


text:
America's Richest Female Entertainers 2016: Madonna, Judge Judy And Taylor Swift Reign Supreme
It turns out the arts do pay—if you happen to be one of the eight lucky entertainers on FORBES' second-annual ranking of America's richest self-made women.
The 60-person list of ceiling crashers, who are worth a combined $53 billion, includes a handful of musicians, a TV personality and two authors.
Madonna lives up to her read »Natalie Robehmed, Forbes Staff


text:
I must confess we once had a white female German Shepherd with a very unique
name. We had always had male pets. Her name: Girldog.
Slugger, Seamus and Braveheart all have simple names, but Mick is fully
Michael Collins and Jack is Jackson Greye.
They all answer to Mr. Pie though. as in Cutie Pie, Honey Pie, Sweety Pie.
----- Original Message -----
From: "Judy L Browning" <email@example.com>
To: <email@example.com>
Sent: Wednesday, July 21, 2004 9:01 PM
Subject: Re: [CHAT] Slugger pic now cat names
> well, starting now & going back to '75, we have
> Maisy slinky grey with white bib & spats, preceeded by
> Cricket black manx,
> Quebec black long haired,
> Bambicita grey "squirrel coated" manx,
> Anna white plush coat blunt tail & face,
> Charlie brown & grey striped Tom with white bib & black feet & ears.
> Maggie orange tiger waif with white nose & bib,
> Tiger grey striped tom with white nose bib & spats,
> Buddy long haired grey & black striped manx tom,
> Rosie dark calico no white,
> Sweet Georgia Brown 5 color calico manx (probably the prettiest cat I ever
> saw & bouncy like the song), & her mom
> Gypsy tortoise shell manx, we had 3 or four litters from her, found homes
> for all.
> From: "Kitty" <firstname.lastname@example.com>
> >
> > OrangeCat, Orange Kitty - don't we have great imaginations when it comes
> to
> > pet names?! :+D
> >
> > Kitty
> ---------------------------------------------------------------------
> Support hort.net -- join the hort.net fund drive!
> http://www.hort.net/funds/
Support hort.net -- join the hort.net fund drive!


text:
March 25, 2011
New theater to open at Magic Valley Mall; existing one there to close
TWIN FALLS, ID — The Odyssey 6 Theater operated by Interstate Amusements at the Magic Valley Mall is slated to close by September, 2011, but local moviegoers will have little time to lament its passing as a new all-digital twelve-plex will open at the mall in December to be operated by California-based Cinema West. The new theater will be Cinema West's first venture into Idaho.
"We feel that the Twin Falls market is under served and there is a lot of room for improvement," Corkill said. "The amenities we'll offer are comparable to what many people experience in larger urban settings. They are amenities that enhance the experience and what people have grown to expect and deserve."
Corkill said the company expects the complex to draw not only TwinFalls residents but people from across southern Idaho.
There is more in this article at Magic Valley, which includes a sketch of the new cinema.
February 15, 2011
AMC closes another former Kerasotes theater in Illinois
PARIS, IL — The Paris Theater a former Kerasotes property, was closed by AMC on February 6. The theater opened in 1924 as the Lincoln and was twinned in 1996. The nearest cinema now available to local residents is in Terre Haute, IN, about twenty-five miles away.
Employees of the theater told the Journal-Gazette newspaper in Mattoon that the theater closed Sunday night.
AMC Entertainment spokesman Justin Scott said Wednesday the Paris theater no longer "competed effectively in the marketplace."
The story is here.
February 2, 2011
Rochester's Chateau 14 closes suddenly
ROCHESTER, MN — The Chateau 14 originally a ten-plex that opened in 2002, was abruptly closed by CineMagic Theatres on January 26. Performances for the day had already begun when staff were told to close the megaplex.
Florida-based Paragon Theaters says it will renovate the lobby, add a bar and lounge, all-digital Sony 4k projection, premier and reserved seating and 3D screens.
The theater will remain closed for a while for renovations and staff training. It's expected to re-open in late February or early March.
There is more in the Post Bulletin.
February 1, 2011
After 87 years, Lakewood's Detroit Theatre closes its doors
LAKEWOOD, OH — Truly a survivor of a past age, the nearly ninety-year-old Detroit Theatre in the Cleveland suburb of Lakewood is closing as of January 30. The theater, opened in 1927, was twinned in 1987.
There is a little more at Cleveland.com
January 31, 2011
Current operator closes the Fox in Taft; removes equipment
TAFT, CA — The most recent operator of the Fox Theatre in Taft has closed the theater and begun the removal of equipment. The mayor is convening a special meeting of the City Council in February to see what action can be taken to stop further removal of furnishings and equipment. The theater had been facing foreclosure last November, but it was thought then that a solution has been found.
Lockwood said that the theater was losing money because of lack of attendance, and that he had finally decided to close the theater.
Lockwood said that he is willing to sell the equipment, fixtures and theater seating if he found a willing buyer.
The recent news about the change of events is here.
January 26, 2011
Laemmele's Music Hall likely to close in April
BEVERLY HILLS, CA — The Laemmele Music Hall triplex is likely to close in April when its lease is up, primarily as a result of declining patronage, though the property's owner is reportedly looking for another operator.
The story appeared in LA Weekly.
January 21, 2011
Loew's 72nd Street closed
NEW YORK, NY — The rumored closing of Loew's 72nd Street East formerly known as Loew's Tower East, finally occurred in December when its lease was not renewed. The theater opened in 1962 as a replacement for a far larger and more elegant Loew's 72nd Theatre that once occupied the site. It was one of the last single screen theaters operating in Manhattan.
The theater is the latest installation on Third Avenue near East 71st Street to close down, as the entire block is now nearly vacant. The Victoria's Secret next door to the theater closed as did a Bath and Body Works on the corner of East 72st Street. The only remaining store, Super Runners Shop, will be vacating soon, its manager John Pier said.
But the businesses weren't forced out by the economic downturn. Their leases simply weren't renewed, Pier said.
The owners of the Tower East building want to renovate the storefronts — most likely turning the four spaces into three, Pier said — and get new tenants.
The full story with some pictures is at DNAInfo.com.
January 10, 2011
Oaks Theater closes
BERKELEY, CA — The 1925 Oaks Theater has closed and the property's owner, John Gordon, is looking again for new operators, preferring to keep the theater as an operating entertainment facility. He says the the most recent operators did not follow through on their business plan and were months behind in rent.
John Gordon, the commercial property developer and realtor who owns the Oaks Theater, served notice to the group in early December that it was in default of its lease since it hadn't paid rent in three months, he said. While running an independent movie theater is a tough business, the theater failed because Merriment Media did not follow through on any of its turnaround plans, according to Gordon.
There is more at SF Gate.
January 6, 2011
Orchard Theatre in Port Orchard closes
PORT ORCHARD, WA — In 2007, The former Plaza Twin Theatre here reopened as the Historic Orchard Theatre but disappointing financial returns caused the current owner, Far Away Entertainment, to close the theater on December 30, 2010. The company may be open to a sale of the theater.
"With a major first-run, multi-screen theater nearby, we felt that a more adult-oriented independent and art film concept was worth trying," he said. "It's been difficult to attract this audience segment on a consistent basis, especially in the evening hours."
As in all of its theaters, Far Away hosted numerous community and non-profit events in Port Orchard for local charities and civic organizations.
There is more in the Port Orchard Independent.
Destinta Theatres closes Independence Plaza 12
HAMILTON, NJ — December 31, 2010 was the last day for the Destinta Independence Plaza 12 which opened in 1999.
Mayor John Bencivengo on Monday night said Hamilton has recently seen positive developments with local businesses but that Destinta's closure "is a result of the national economy" where consumers are putting a "tight hold on discretionary spending."
"It's never a good moment when a business is closing, especially when it's in Hamilton," Bencivengo said. "We hope that (the Independence Plaza) center does well, and we hope it gets filled again, that particular movie theater."
There is more in the Trentonian.


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Professional and Scientific Council
The Iowa State University Professional and Scientific Council serves as a resource and an advocate for P&S employees. The Professional and Scientific Council is a representative body elected by, and responsible to, P&S employees at Iowa State University. The council identifies the needs of its constituents, provides information and advice in response to those needs, and recommends policies and procedures to the administration that benefit P&S employees and assist in fulfilling the mission of Iowa State University.
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Goldstar Member's Event Journal
Thursday March 13, 2014 / 7:30pmChris Kattan
2 tickets
General Admission (Center and Side)
How was your experience?
Obviously Chris Kattan was great. Exactly what you'd expect from him. However I was pretty disappointed in the seats. We were sold (side/center) seats. Assuming there would be a limited amount of seats for the center we got there super early to make sure we got some. Turns out there was no center seats available. Which means what they sold us was a lie. Very disappointed for my first Goldstar experience.


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In this third installment of Q&A's, Zack Cooper gives a brief overview of the scoring system in Blacklist works for all three play styles.
Splinter Cell: Blacklist economy system trailer
Tom Clancy's Splinter Cell: Blacklist PS3
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- 21 Here's what the previews are saying about Mass Effect Andromeda
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Out of the shadows.
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Erste Group Bank AG was founded in 1819 as the first Austrian savings bank. Now around 47,000 employees are serving 16.6 million clients in more than 2,800 branches in 7 countries. Erste Group is one of the largest financial services providers in Central and Eastern Europe.
Erste Bank a.d. Novi Sad is the member of Erste Group, financial institution with the tradition longer than 190 years, for which, the corporate social responsibility is, from the very beginning, an integral part of corporate philosophy and long-term business strategy. For us, the corporate social responsibility is a strategic framework for company governance, based on investment in long-term and stable relations with all key stakeholders: employees, clients, and fellow citizens in local communities we operate in, and on a commitment to provide active contribution to the development and welfare of the society where we are an inseparable part.
Experience has shown that, in the period of global integrations, it is not possible to apply usual operation methods: local crises very quickly become global ones, and economic issues are closely related to the ecological and social ones; the consequence is that corporate, public, and non-profit sector may not act separately from each other to adequately respond to them. The corporate social responsibility is an approach enabling us to, acting in partnership with other sectors, more successfully face the challenges posed by such market. It is the concept stimulating us to be innovative, to encourage sustainable development, responsibly manage risk, and decrease costs, thus achieving better operational results for our clients and company, as well as for the communities we operate in.
The latest Erste Bank a.d. Novi Sad Corporate Social Responsibility Strategy is adopted for the period from 2015 to 2019, and it is built on the following principles:
The areas through which Erste Bank Corporate Social Responsibility Strategy is implemented include:
Special characteristic of our engagement includes the efforts made to promote the corporate social responsibility concept in Serbia, both on our own example and through intensive cooperation with partners from public and non-profit sector. In accordance with such policy, our Bank became a member of the initiatives advocating the corporate social responsibility in Serbia, such as United Nations Global Accord for Serbia and and Forum for Responsible Business.
Reporting on the corporate social responsibility is an integral part of a broad process we are committed to on a long-term basis – continuing improvement of the Corporate Social Responsibility Strategy and socially responsible practices, in accordance with the needs of target groups and interest groups and individuals, i.e. all of our stakeholders, and with the market requirements and social environment. Since 2008, we have published annual reports on the social responsibility on a regular basis. Our objective is to, in a clear and transparent manner, present our stakeholders all of the activities and initiatives realised during one year, as well as to announce forthcoming plans and programmes.
Any additional questions, comments, and suggestions on the topic of the social responsibility may be sent by e-mail to the address: firstname.lastname@example.com


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Comment profit! (Score 1) 549
If you read this post, you owe me one million dollars. Mwah ha ha!
He wasn't allowed to use a computer that had 'encryption,
Want to log in to your baking site with ssl. Sorry kid.
Try MS SteadyState
and better yet: in addition to just using SteadyState on the whole disk, make regedits to keep 'Documents and Settings' on a separate partition where SteaedyState is off so the users can actually save something to their hard drive that won't be lost on a reboot, which IMHO is much more useful.
"God is a comedian playing to an audience too afraid to laugh." - Voltaire


text:
The interaction between C5a and sphingosine-1-phosphate in neutrophils for antineutrophil cytoplasmic antibody mediated activation
DOI: 10.1186/ar4604
© Hao et al.; licensee BioMed Central Ltd. 2014
Received: 9 January 2014
Accepted: 1 July 2014
Published: 7 July 2014
C5a plays an crucial role in antineutrophil cytoplasmic antibody (ANCA)-mediated neutrophil recruitment and activation. The current study further investigated the interaction between C5a and sphingosine-1-phosphate (S1P) in neutrophils for ANCA-mediated activation.
The plasma levels of S1P from 29 patients with ANCA-associated vasculitis (AAV) in active stage and in remission were tested by enzyme-linked immunosorbent assay (ELISA). The generation of S1P was tested in C5a-triggered neutrophils. The effect S1P receptor antagonist was tested on respiratory burst and degranulation of C5a-primed neutrophils activated with ANCA.
The plasma level of circulating S1P was significantly higher in patients with AAV with active disease compared with patients in remission (2034.2 ± 438.5 versus 1489.3 ± 547.4 nmol/L, P < 0.001). S1P can prime neutrophils for ANCA-induced respiratory burst and degranulation. Compared with non-triggered neutrophils, the mean fluorescence intensity (MFI) value for CD88 expression was up-regulated significantly in S1P-triggered neutrophils. S1P receptor antagonist decreased oxygen radical production in C5a primed neutrophils induced by ANCA-positive IgG from patients. Blocking S1P inhibited C5a-primed neutrophil migration.
S1P triggered by C5a-primed neutrophils could further activate neutrophils. Blocking S1P could attenuate C5a-induced activation of neutrophils by ANCA. The interaction between S1P and C5a plays an important role in neutrophils for ANCA-mediated activation.
Antineutrophil cytoplasmic autoantibody (ANCA) is closely associated with systemic small vessel vasculitis characterized by segmental vessel wall necrotizing inflammation and a paucity of immunoglobulin deposition [1]. Patients with ANCA-associated vasculitis (AAV) can on the basis of clinical and pathological features be classified as granulomatosis with polyangiitis (GPA, previously named Wegener's granulomatosis), microscopic polyangiitis (MPA) and eosinophilic granulomatosis with polyangiitis (EGPA, previously named Churg-Strauss syndrome). ANCAs against either proteinase-3 (PR3) or myeloperoxidase (MPO) are hallmarks of AAV [2].
Cumulating evidence suggests that ANCA-induced neutrophil activation plays a crucial role in the pathogenesis of AAV [3–7]. In an anti-MPO antibody-induced mouse vasculitis model, ANCAs are proven to be pathogenic [8]. Furthermore, neutrophils are the primary effector cells in AAV [8, 9].
Recent studies, both in the mouse model and in humans, demonstrated that complement activation via the alternative pathway is indispensable in the development of AAV [10–14]. Schreiber et al. further found that recombinant C5a dose-dependently primes neutrophils for ANCA-induced respiratory burst. As such, the interaction between C5a and the neutrophil C5a receptor (C5aR, namely, CD88) compose an amplification loop, and is one of the central contributing factors in ANCA-mediated neutrophil recruitment and activation [15].
Regardless of the fact that C5a-C5aR axis may represent an attractive target for immunosuppressive therapy, little is known about the intracellular molecular mechanisms responsible for the C5a-triggered physiological events or key molecules in ANCA-mediated activation of C5a-primed neutrophils [16].
It has become clear that sphingolipids are sources of important signaling molecules [17]. Sphingosine-1-phosphate (S1P) is a potent bioactive sphingolipid metabolite that regulates diverse cellular processes that are important for inflammation and immune responses. Various activated plasma-membrane receptors, such as the platelet-derived growth factor (PDGF) receptor [17, 18], FcϵRI and FcγRI antigen receptors [19], tumor necrosis factor receptor 1 (TNFR1) [20–22] and N-formyl-methionyl-leucyl-phenylalanine (FMLP) receptor [23], respectively upregulate sphingosine kinase (Sphk) and generate S1P. S1P is not only an agonist of five specific G protein-coupled S1P receptors (S1PR1-5) that activate diverse downstream signaling pathways, but also has important intracellular (second messenger) actions [22, 24–26]. It had previously been demonstrated that S1P plays an important role in autoimmune diseases, such as rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis and asthma [21, 22, 27–29]. In addition, Sphk1 plays the crucial role in regulating the balance between expression of CD88 and C5a receptor-like 2 (C5L2) in endotoxin-induced lung inflammatory injury [30]. Antisense knockdown of sphk1 in human macrophages inhibits C5a receptor-dependent signal transduction [31].
Therefore, we hypothesized that S1P triggered by C5a-primed neutrophils could further activate neutrophils; the interaction between S1P and C5a might be involved in ANCA-mediated neutrophils respiratory burst and degranulation.
Patients and blood samples
Plasma samples from 29 consecutive patients with active AAV at initial onset, diagnosed at Peking University First Hospital from 2010 to 2011, were collected before immunosuppressive treatment. All these patients met the Chapel Hill Consensus Conference (CHCC) nomenclature of AAV [2]. Patients with secondary vasculitis or with comorbid renal diseases, such as anti-glomerular basement membrane (GBM) nephritis, were excluded. All the above-mentioned 29 patients received corticosteroids and cyclophosphamide for the induction therapy and achieved remission. Plasma samples from these patients at the remission stage were also collected at their regular ambulatory visits. The time of sampling was 11.5 ± 3.0 months after remission was achieved. When sampling at remission, all of them still received oral azathioprine for maintanence therapy. Twenty-nine age- and gender-matched healthy blood donors were enrolled as normal controls. The blood samples from patients and controls were drawn into ethylene diamine tetraacetic acid (EDTA) tubes and put on ice immediately. The blood samples were centrifuged at 2000 g for 15 minutes at 4°C within 30 minutes after collection and the plasma samples were stored at -70°C until use. Disease activity of AAV was assessed according to the Birmingham vasculitis activity score (BVAS) [32]. Remission was defined as 'absence of disease activity attributable to active disease qualified by the need for ongoing stable maintenance immunosuppressive therapy', as described previously [33].
Preparation of immunoglobulin (Ig)G
Normal IgG and ANCA-positive IgG were prepared from plasma of normal volunteers and patients with active MPO-ANCA- or PR3-ANCA-positive primary small vessel vasculitis, using a High-Trap-protein G column on an AKTA-FPLC system (GE Biosciences, San Francisco, CA, USA). None of these patients had dual positivity of PR3-ANCA and MPO-ANCA. Preparation of IgG was performed according to the methods described previously [34, 35]. Containers and solution for IgG preparation did not contain lipopolysaccharide (LPS). The concentration of LPS in ANCA-positive IgG was below 0.1 ng/ml.
Neutrophil isolation
Neutrophils were isolated from heparinized venous blood from healthy donors by density gradient centrifugation on Lymphoprep (Nycomed, Oslo, Norway). Erythrocytes were lysed with ice-cold ammonium chloride buffer, and neutrophils were washed in Hank's balanced salt solution (HBSS) without Ca2+/Mg 2+ (HBSS-/-; Chemical reagents, Beijing, China). The purity of the neutrophils was above 95%. Neutrophils were then suspended in HBSS with Ca2+/Mg2+ (HBSS+/+; Chemical reagents) to a concentration of 2.5 × 107 cells/ml and used for ANCA antigen translocation analysis, respiratory burst measurements, neutrophil degranulation and detection of S1P in neutrophil supernatant [35]. This research was in compliance with the Declaration of Helsinki and approved by the clinical research ethics committee of the Peking University First Hospital. Written informed consent was obtained from each participant.
Inhibition of the S1P receptor
Previous studies have showed that neutrophils express S1PR1, 4 and 5 [36]. VPC23019 (Tocris, Louis, USA) is a specific antagonist for S1P receptor 1 and 3 [37]. CYM50358 (Tocris) is a specific antagonist for S1P receptor 4 [38]. FTY720 is a structural analog of sphingosine, as well as being phosphorylated by Sphk, which then acts with four S1P receptors (S1P1, S1P3, S1P4 and S1P5) [28]. FTY720 (Selleck, Houston, USA) could abolish the biological effect of S1P and cover all types of S1P receptors on neutrophils. The concentration of the above-mentioned S1P receptor antagonists was first investigated. Cells were incubated with VPC23019 or CYM50358 at different doses (25nM; 50nM; 100nM; 200nM) for 15 minutes. VPC23019 and CYM50358 inhibited S1P-primed neutrophils for ANCA-induced activation at 100nM for 15 minutes. The highest inhibition rates of VPC23019 and CYM50358 were 26% and 27%, respectively. The highest inhibition rate of FTY720 (50nM, 15 minutes) in S1P-primed neutrophils for ANCA-induced activation was 68%.
where MFI is the mean fluorescence intensity.
Membrane expression of PR3 and MPO on neutrophils after priming
Flow cytometry was used to evaluate PR3 and MPO expression on neutrophils. Cells were incubated with C5a (100 ng/ml) (Biovision, San Francisco, CA, USA) or buffer control for 45 minutes at 37°C. For S1P priming groups, cells were incubated with S1P (100, 500 and 1000 nmol/L) (Sigma-Aldrich, Louis, USA) or buffer control for 30 minutes at 37°C. Doses for S1P stimulation were all above the dissociation constant for the receptors. All further steps were performed on ice and washing steps were carried out using HBSS +/+containing 1% bovine serum albumin (BSA). Neutrophils were incubated with 0.5 mg/ml heat-aggregated goat IgG for 15 minutes to saturate Fcγ receptors. Next, cells were stained with a saturating dose of mouse monoclonal IgG1 antibody directed against human PR3 (Clone number: WGM2) or MPO (Clone number: 2C7) (Abcam, Cambridge, UK) or with an irrelevant IgG1 control antibody (Biolegend, CA, USA) for 30 minutes. Neutrophils were then incubated with phycoerythrin (PE)-conjugated goat anti-mouse antibody (Abcam, Cambridge, UK) in the presence of 0.5 mg/ml heat-aggregated goat IgG. Fluorescence intensity of PE was analyzed using flow cytometry assessment of ANCA-antigen expression. Samples were analyzed using the FACScan (Becton Dickinson, Heidelberg, Germany). Neutrophils were identified in the scatter diagram, and data were collected from 10,000 cells per sample. The level of PR3- or MPO-expression was calculated as the MFI of specific binding of the isotype control antibody. For the inhibition test, cells were pre-incubated with FTY720 (50nM) or its vehicle, as control, followed by other treatments [35].
Detection of MPO in neutrophil supernatant by ELISA
MPO in the S1P or C5a-primed neutrophil supernatant was tested by ELISA using a commercial kit (USCNK, Wuhan, China). Cells were incubated with S1P (100, 500 and 1000 nmol/L) (Sigma-Aldrich) or buffer control for 30 minutes at 37°C. Supernatant fluids were collected and used for ELISA analysis. In brief, the microtiter plate provided in this kit has been pre-coated with an antibody specific to MPO. The cloaked antibody was a monoclonal antibody in this assay. The detection antibody was a polyclonal antibody. Supernatants of neutrophils at dilutions of 1:200 and standards were then added to the appropriate microtiter plate-wells with a biotin-conjugated antibody preparation specific for MPO. Next, Avidin conjugated to horseradish peroxidase (HRP) was added to each microplate well and incubated. After 3,3′-5,5′ tetramethylbenzidin (TMB) substrate solution was added, only those wells that contained MPO, biotin-conjugated antibody and enzyme-conjugated Avidin would exhibit a change in color. The enzyme-substrate reaction was terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MPO in the samples was then determined by comparing the optical density (OD) of the samples to the standard curve.
Detection of S1P by ELISA
S1P was tested by ELISA using a commercial kit (Echelon,Utah, USA). The 96-well microtiter plate was coated with S1P and blocked to reduce non-specific binding. Then we mixed the S1P standard and samples with the anti-S1P antibody before adding the mixture to the S1P-coated plate. The antibody competes for binding to S1P bound to the plate or in the sample. Following an incubation and plate wash, streptavidin-HRP was added to the plate and bound to all anti-S1P antibodies (labeled with biotin) bound to the plate. After an additional incubation and plate wash, TMB substrate was added to the plate and the reaction stopped by the addition of sulfuric acid. The absorbance at 450 nm was measured. The concentration of S1P in the samples was determined by comparison to the standard curve [39].
Measurement of respiratory burst by oxidation of dihydrorhodamine (DHR) to rhodamine
The generation of reactive oxygen radicals was assessed using DHR. This method was based on the fact that reactive oxygen radicals cause an oxidation of the non-fluorescence DHR to the green fluorescence rhodamine. Isolated neutrophils were gradually warmed to 37°C and incubated with 0.05 mM DHR123 (Sigma-Aldrich) for 10 minutes at 37°C. Sodium azide (NaN3) (2 mM) was added in order to prevent intracellular breakdown of H2O2 by catalase. Then, neutrophils were primed with S1P for 30 minutes or C5a for 45 minutes at 37°C and incubated with patient-derived ANCA-positive IgG (200 μg/ml), normal IgG for 1 h at 37°C. The reaction was stopped by addition of 1 ml of ice-cold HBSS/1% BSA. Samples were kept on ice and analyzed using a FACScan. Neutrophils were identified in the scatter diagram, and data were collected from 10,000 cells per sample. The shift of green fluorescence in the FL-1 mode was determined. For each condition, the MFI (representing the amount of generated reactive oxygen radicals) was reported [34, 35].
ANCA-activated S1P or C5a-primed neutrophils degranulation
Lactoferrin, an iron binding multifunctional glycoprotein that was an abundant component of the specific granules of neutrophils, was considered as a biomarker of neutrophil degranulation [40–42]. Neutrophils were stimulated with S1P or C5a followed by stimulation with MPO-ANCA-positive IgG or PR3-ANCA-positive IgG, normal IgG or buffer control for 1 h, respectively. Cells were pre-incubated with the S1P receptor antagonist or its vehicle, DMSO, as control for 15 minutes on ice before the priming. Lactoferrin in the neutrophil supernatant was tested by ELISA using a commercial kit (USCNK) as a measure of neutrophil degranulation. The ELISA procedure of measuring lactoferrin was as described previously [43]. The concentrations of lactoferrin in the samples were then determined by comparing the OD value of the samples to the standard curve.
Membrane expression of CD88 on neutrophils
Flow cytometry was used to evaluate CD88 expression on neutrophils. Cells were incubated with S1P (500 nmol/L) (Sigma-Aldrich), supernatant of C5a- stimulated neutrophils or buffer control for 30 minutes. All further steps were carried out using HBSS +/+containing 1% BSA. Next, cells were stained with a saturating dose of PE-conjugated mouse monoclonal IgG1 antibody directed against human CD88 (Biolegend, CA, USA) or with an irrelevant IgG1 control antibody (Biolegend, California, USA) for 30 minutes. Fluorescence intensity of PE was analyzed using flow cytometry assessment of CD88 expression. Samples were analyzed using a FACScan (Becton Dickinson, Heidelberg, Germany). Neutrophils were identified in the scatter diagram, and data were collected from 10,000 cells per sample. The level of CD88 expression was calculated as MFI of specific binding of the isotype control antibody.
Neutrophil migration
To test the effect of S1P receptor antagonist on the C5a-induced neutrophil migration, neutrophils were preincubated with 50nM S1P receptor antagonist or vehicle control for 15 minutes: 4 × 105 cells were loaded in the upper chamber of a Transwell insert (Corning, NY, USA) with 3.0-μm pores in 12-well plates. C5a (100 ng/ml) was placed in the lower chamber. The plates were then incubated at 37°C with 5% CO2 for 90 minutes. Neutrophils without treatment of S1P receptor antagonist in the upper chamber or wells without C5a in the lower chamber were used as the controls. The number of neutrophils that migrated across the filter was counted using FACS [44].
Statistical analysis
The Shapiro-Wilk test was used to examine whether the data were normally distributed. Quantitative data were expressed as means ± SD (for data that were normally distributed) or median and range (for data that were not normally distributed). Differences in quantitative parameters between groups were assessed using the t-test (for data that were normally distributed) or Mann-Whitney U-test (for data that were not normally distributed) as appropriate. Differences were considered significant at P <0.05. Analysis was performed with SPSS statistical software package (version 16.0, Chicago, IL, USA).
Plasma levels of S1P were elevated in AAV patients in the active stage compared with remission
S1P increased translocation of ANCA antigens
S1P-primed neutrophils for ANCA-induced respiratory burst and degranulation
S1P receptor antagonist inhibited C5a-primed neutrophils for ANCA-induced respiratory burst and degranulation
Pretreatment with S1P receptor antagonist significantly reduced MPO-ANCA-positive IgG-induced and PR3-ANCA-positive IgG-induced lactoferrin release. The lactoferrin concentration increased from 1,387.5 ± 71.2 ng/ml in the non-primed neutrophil supernatant to 3,059.8 ± 109.0 ng/ml in C5a-primed neutrophils induced by MPO-ANCA-positive IgG supernatant (P <0.001), and decreased to 1,966.3 ± 72.3 ng/ml upon pre-incubation with S1P receptor antagonist (compared with that without the antagonist, P < 0.001, the inhibition rate was 65.2 ± 3.1%). In C5a-primed neutrophils induced by PR3-ANCA-positive IgG, the lactoferrin concentration in the supernatant increased from 1,387.5 ± 71.2 ng/ml in untreated cells to 3,150.3 ± 41.9 ng/ml (P <0.001), which decreased to 1,982.3 ± 64.7 ng/ml upon pre-incubation with S1P receptor antagonist (compared with that without the antagonist, P <0.001, the inhibition rate was 66.3 ± 5.4%) (Figure 4C).
Supernatant of C5a-stimulated neutrophils primed fresh neutrophils for ANCA-mediated respiratory burst and degranulation
Pretreatment with S1P receptor antagonist significantly reduced MPO-ANCA-positive IgG-induced and PR3-ANCA-positive IgG-induced lactoferrin release. The lactoferrin concentration increased from 427.6 ± 104.0 ng/ml in the non-primed neutrophils supernatant to 1,105.0 ± 380.1 ng/ml in supernatant-primed neutrophils induced by MPO-ANCA-positive IgG supernatant (P <0.001), and decreased to 731.0 ± 201.9 ng/ml upon pre-incubation with S1P receptor antagonist (compared with that without the antagonist, P < 0.001). In supernatant-primed neutrophils induced by PR3-ANCA-positive IgG, the lactoferrin concentration increased from 427.6 ± 104.0 ng/ml in untreated cells to 1,110.2 ± 389.2 ng/ml (P <0.001), which decreased to 750.4 ± 224.1 ng/ml upon pre-incubation with S1P receptor antagonist (compared with that without the antagonist, P <0.001) (Figure 5B).
S1P or supernatant of C5a-stimulated neutrophils upregulated CD88 expression on neutrophils
S1P receptor antagonist inhibited C5a-induced neutrophil migration
To clarify the involvement of S1P on C5a-induced neutrophil migration, we investigated the effect of S1P receptor antagonist on neutrophil migration. Pre-incubation with S1P receptor antagonist significantly inhibited C5a-induced neutrophil migration (the number of migrated neutrophils was 110.5 ± 20.0 versus 200.0 ± 13.5, P <0.01) (Figure 6B).
ANCA-induced neutrophil respiratory burst and degranulation are important contributors to the development of AAV. Recent studies, both in the mouse model and in humans, suggest that complement activation is involved in the pathogenesis of AAV [10–15, 45–47]. In particular, the interaction between C5a and C5aR (CD88) plays a central role in ANCA-mediated neutrophil recruitment and activation [15]. Our previous study confirmed this observation by demonstrating that purified recombinant C5a increased ANCA antigen translocation and ANCA-mediated respiratory burst in C5a-stimulated neutrophils [45]. However, the intracellular molecular mechanism triggered by C5a in neutrophils has not been fully identified.
It is becoming more evident that certain classes of membrane lipids can be modified in a regulated manner to generate bioactive lipid second messengers. S1P is generated by the conversion of ceramide to sphingosine by the enzyme ceramidase and the subsequent conversion of sphingosine to S1P, which is a potent bioactive sphingolipid metabolite that regulates inflammation and immune responses [48].
Several studies have reported that sphingosine acts as an endogenous regulator of neutrophil functions [23, 49, 50]. It was reported that low concentration of S1P promotes inflammatory cell chemotaxis [51, 52]. Furthermore, it has been proposed that Sphk regulates neutrophil priming to provide an essential defense against infections [50], and to mediate neutrophil inflammatory responses [23, 50]. The current study found that the level of circulating S1P was significantly higher in AAV patients with active disease compared with those in remission. More importantly, S1P was found to prime neutrophils for ANCA-induced respiratory burst and degranulation. Therefore, we speculated that S1P might promote the development of inflammation and disease activity of AAV.
In our study, it was found that S1P receptor antagonist downregulated C5a-induced neutrophil migration and significantly attenuated C5a-primed neutrophils for ANCA-induced respiratory burst and degranulation, with an inhibition rate of about 80%, which suggests that the S1P played an important role in C5a-primed neutrophils for ANCA-mediated activation. In addition, S1P upregulated CD88 expression on neutrophils, which suggests that there may be an S1P-C5a loop in ANCA-induced neutrophil activation. It was reported that S1P is involved in several immune responses of C5a, which C5a rapidly stimulates the generation of S1P, Sphk1 activity, and membrane translocation of Sphk1 in human monocyte-derived macrophages [53–56]. Bachmaier et al. reported that Sphk1 regulates the balance between expression of CD88 and C5L2 on phagocytes in experimental lung inflammatory injury [30].
In the previous study, it was found that reactive oxygen species increased in TNFα-primed human monocytes for ANCA-induced activation [57]. Presumably, S1P might induce a similar effect on monocytes, which is of special interest for further investigation.
antineutrophil cytoplasmic antibody-associated vasculitis
antineutrophil cytoplasmic antibody
bovine serum albumin
Birmingham vasculitis activity score
- C5aR:
C5a receptor
C5a receptor-like 2
ethylene diamine tetraacetic acid
eosinophilic granulomatosis with polyangiitis
enzyme-linked immunosorbent assay
fluorescence activated cell sorting
granulomatosis with polyangiitis
Hank's balanced salt solution
horseradish peroxidase
- Ig:
mean fluorescence intensity
microscopic polyangiitis
optical density
platelet-derived growth factor
- Sphk:
sphingosine kinase
3,3′-5,5′ tetramethylbenzidin
tumor necrosis factor
tumor necrosis factor receptor 1.
This study is supported by a grant of Chinese 973 project (number 2012CB517702), two grants of the National Natural Science Fund (numbers 81370829 and 81321064), and the Research Fund for the Doctoral Program of Higher Education of China (number 20120001110018).
Authors' Affiliations
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Graphic Design - Entire QSR Restuarant menu's and Direct Marketing material
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The Voice must have one of the most complicated formats of any talent contest on TV. As if the role of the spinning red chairs wasn't baffling enough, there are four distinct audition stages before the public gets to vote on acts. This Saturday night it was the final show of what are called the "Battle Rounds", in which the celebrity coaches whittle down their acts by having them sing against each other in competitive duets. The rejected singers then have a chance to be "stolen" by a rival coach.
What's the art to matching singers in battle pairs? Is it to choose singers with different styles so that they bring out the best in each other? To compare close matches? Or should the coaches use the battles as a way to eliminate acts they're having second thoughts about? After this marathon 130-minute episode, we were none the wiser.
Amelia and Rachael must have been paired in the category for 16-year-old girls who have been made up to look like 35-year-old women. This is something of a trend on these shows. Soon they'll be sending teenage contestants on stage with prosthetic wrinkles and dyed grey hair. Vicky and Melissa were facing off in the rock-chicks-with-lip-piercing category – and it was Miles vs Max in the battle for the title of Man of Medium Height with a Guitar and an "M" Initial.
The highlight of the evening was Obama look-a-like Femi and Iesher, the spit of pop rapper Nicki Minaj, in the People Who Look Like Other People category. They did a version of "Stop, Look, Listen (to Your Heart)" which gave Kylie a chance to use her final steal, Tom Jones a chance to name-drop Diana Ross and Iesher a chance to show off her stony singing expression. Everyone was happy – except Iesher, obvs. Will.i.am says she needs to emote more, but I disagree. Those perma-frozen facial muscles only emphasise the effortlessness of her talent.
The battle round's real format issue is not the illogical match-ups. It's that it just takes far too long. Two hours and ten minutes is more than enough time for the earnest thank-yous to sound clichéd, for the hysterical meltdowns to get old hat and for the paucity of will.i.am's vocabulary to become apparent. "Awesome" is a fine adjective, but it will not do for every situation. Worst of all, when Tom Jones does one of his boggle-eyed double-takes, it proved impossible to tell if he was surprised by an auditionee's bum note, or had just been rudely awoken from an afternoon nap.Reuse content


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HP CF031A. Type: Cartridge, Page yield: 12500 pages, Printing colours: Cyan. Package width: 39 cm, Package height: 21.4 cm, Package weight: 1.84 kg
HP 646A Cyan LaserJet Toner Cartridges keep your business productivity high. Original HP print cartridges with HP ColorSphere technology deliver consistent, professional colour on marketing, financial, design and engineering documents.


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Welcome to Fred Hill Mechanical
Fred Hill Mechanical is a third generation family owned and operated business that began in 1944. From its origination the company has provided residential, commercial and industrial, Plumbing, Heating & Air Conditioning, Sheet Metal and Retail Sales services to the Modesto and surrounding areas.
Residential Services
Summers in the Central Valley are warm, our service technicians make sure your A/C is doing it's job while at the same time saving you money.
Commercial Services
We are equipped to handle both Residential and Commercial needs. With over 65 years of experience at hand.
We provide repair services and scheduled maintenance programs to make sure your heating system is running efficiently and effectively.
Fred Hill Mechanical, Inc. services the Central Valley including Modesto, Ceres, Oakdale, Ripon, Salida, Manteca, Escalon and Turlock.


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Not much has changed for Two and a Half Men over the years. The characters remain themselves, the gags remain completely inappropriate for network television, and it's always consistently laugh out loud funny. Season four shows no decline in the laughs-per-episode quotient.
The season picks right up from the third, with a botched Vegas wedding. Alan's his usual overly depressed self, Charlie is still womanizing, and Jake… well, Jake is changing. He's slowly getting older, and a few episodes like "It Never Rains in Hooterville" do a great job of making the adjustment.
There's little question this series is formulaic. Nearly every episode features Charlie (Charlie Sheen) with another woman, Alan (Jon Cryer) reacting, with Jake (Angus T. Jones) stuck in the middle. The difference is that these are truly wonderful characters, branching out to every possible extreme. The performances, especially Jon Cryer who is always at his peak in each episode, are memorable and deserving of accolades.
There's little to complain about when it comes to Two and Half Men. Aside from those who find the show offensive to whatever morals they may have (and can't get past it even though it's a TV show), it's hard to see anyone not finding this funny. It's not hard to see this end up being one of the longer running comedies on television.
Like the show, the DVD transfers have been consistent as well: fairly awful. Season four is compounded by edge enhancement, an overall murky look, lackluster detail, and inconsistent flesh tones. Compression artifacts are constantly a problem, and are quite distracting. Primary colors fare well and at the very least show some consistency.
It's a TV show. Hence, the DVD is presented in basic stereo, and lacks much of anything worthy of discussion. Dialogue is clear, and the laugh track sounds fine. Oh, and the theme song offers a decent amount of bass as well.
Extras include a basic gag reel that runs eight minutes. It's loaded with some quality line flubs and a couple of physical mishaps. Two Men Talk About Two and a Half Men is a 13 minute featurette with the show's co-creators. They talk openly about the content of the show, working around the censors, and the characters they've created. Finally, two of the episodes carry commentaries (one episode has the cast, the other has the creators), though why they chose these specific ones is never really known.
While it is fairly obvious, the opening theme song is performed not by the main cast, but studio singers. The real cast simply lip-sync the lyrics.Powered by Sidelines


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Can a gluten free person get a good meal at this restaurant?
Is this restaurant good for large groups?
Is this a pub?
Is this restaurant good for dinner?
Is this restaurant good for business meetings?
Does this restaurant have parking?
Is this restaurant good for breakfast?
Does this restaurant accept reservations?
Is this restaurant good for lunch?
Does this restaurant offer free wifi?
Thanks for helping!
The portions for us were huge. We had the veggie pizza that was pretty good. It's a really dark dining room could do with brightening up. Staff were good and friendly.. It's handy if you have a late flight as its open until11pm
Food was decent. Service was good. Nothing special on the menu. Pricing was on the high side but should be expected as it is inside the hotel I was staying...
Not the best. I'll admit I didn't have the pizza, which was probably passable. The appetizers were average at best (artichoke dip, calamari) and my main, which was the oven roasted chicken, was overloaded with spices and almost inedible.
Not unlike many hotel-resident restaurants (this one at Crowne Plaza).
Food is overpriced for what you get quality wise. The waiter at my table seemed bother by any request small or large. Parking is a bit of a pain.
Wide variety to the menu, excellent service (and it was a super busy evening). Good healthy and vegetarian options for those who prefer it...
Breakfast buffet is pretty good. Plenty of choices. Fresh fruit was very good (strawberries, pineapple, watermelon).
The food and service was excellent. There were 5 of us that had just arrived at the hotel and we were starving. They were quick to seat us and to make sure that we had drinks and menus. We had an additional person join us mid-way through and the staff didn't blink an eye.. Great customer service.
Enjoyed a delicious pizza dinner. I would highly recommend this restaurant. The waitstaff was very attentive and eager to be sure our dining experience was satisfactory.
I have traveled and stayed at the Crowne Plaza Rosemont twice. Both times for a busy conference. The food is good, but this place never seems to be prepared for busy. Just OK service.
I stopped in for lunch and had the steak bomb and a beer. The food was very good including the he made chips. The service was a bit slow and the restaurant was way too hot and humid. After ordering it was a good 10 minutes before the beer arrived. It was probably another one 10-15 minutes before the food...
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How much does the weekday breakfast buffet cost?
are reservations required for like groups of 8?
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by Keith A. Johnson
PLEASE NOTE: Marketplace items do NOT qualify for free shipping.
This book offers a broad overview of the branches of phonetics for students with no prior knowledge. It builds on the basics, beginning with technical terms required for describing speech and transcription symbols.
Keith A. Johnson


text:
"We should not be afraid sometimes to confront beauty and horror."
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How does contemporary art address the idea of consumption? How do artists question commonly held assumptions about commerce, mass media, and consumer society? The "Art in the Twenty-First Century" documentary "Consumption" explores these questions through the work of the artists Barabra Kruger, Michael Ray Charles, Matthew Barney, Andrea Zittel, and Mel Chin.
Major underwriting for "Art in the Twenty-First Century" Season One provided by: Robert Lehman Foundation, Inc; Public Broadcasting Service; National Endowment for the Arts; Corporation for Public Broadcasting; Rockefeller Brothers Fund; Agnes Gund and Daniel Shapiro; The Allen Foundation for the Arts; The Broad Art Foundation; The Jon and Mary Shirley Foundation; Bagley Wright Fund; The Rockefeller Foundation; The Andy Warhol Foundation for the Visual Arts; The Horace W. Goldsmith Foundation; The Foundation-to-Life.done reading


text:
Spinach Noodle Casserole
|Frozen spinach
|10 Ounce, thawed, drained (1 Package)
|Half and half
|1⁄2 Cup (8 tbs)
|1 , finely chopped
|Chopped garlic
|2 Tablespoon
|Olive oil
|2 Tablespoon
|Fresh baby spinach
|7 Ounce
|1⁄4 Cup (4 tbs) (1/2 Stick)
|Sour cream
|1 Cup (16 tbs)
|Cream cheese
|8 Ounce, softened
|Wide egg noodles
|16 Ounce, cooked, drained (Extra Wide Variety)
Puree the thawed frozen spinach and half and half in a blender.
Saute the onion and garlic in the olive oil in a skillet over medium-low heat for 5 to 10 minutes or until tender.
Add salt, pepper and the spinach.
Saute for about 5 minutes or until spinach wilts.
Stir in the butter, sour cream, cream cheese and half-and-half mixture; cook for about 5 minutes or until mixture is uniform and melted, stirring frequently.
Spoon into a greased 9x13 inch baking dish.
Stir in the noodles.
Bake, uncovered, at 375 degrees for 10 minutes or until hot.
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 4020 Calories from Fat 2055
% Daily Value*
Total Fat 235 g360.9%
Saturated Fat 117.5 g587.4%
Trans Fat 0.3 g
Cholesterol 915.8 mg305.3%
Sodium 1462.1 mg60.9%
Total Carbohydrates 388 g129.3%
Dietary Fiber 31.2 g124.9%
Sugars 34.6 g
Protein 105 g210.6%
Vitamin A 1071.1% Vitamin C 165.6%
Calcium 143% Iron 118.8%
*Based on a 2000 Calorie diet