text:
Select the area you wish to focus using polygon lasso tool. Once the area is completely selected, right click on that area and select Inverse so that outer area gets selected now select filter on the menu bar and select blur, which opens a sidemenu, select radial blur option select the range of blur carefully and click, u should be able to see changes. … [Read more...]
How to Create Reflection Text With Photoshop
Here is simple tutorial to create realistic look of reflection text in Photoshop in a few easy steps. Step 1 Open Photoshop and create a new document with the sizes set to around 350x 200 and the background should be set to white. Step 2 Choose the Text tool(T) and write out whatever the text you want to reflect is, here I have written Geekyard.com Step 3 Now goto … [Read more...]
50+ Great Photoshop Text Effect Tutorials
Here is very good collection of 50 Great Photoshop Text Effect Tutorials from Hongkiat.com. Learn how to create stunning text effects using Photoshop. 50 Stunning Photoshop Text Effect Tutorials Text In Stitches Colorful Glowing Text Effect Spray Paint Text Bling Bling Simple Gold Text Effect Cheese Text Effect Reflective Glass Tutorial (Video … [Read more...]
How To Remove Wrinkles in Photoshop
Would you like to reverse the clock and look younger with wrinkle free face? Well, here is a simple tutorial to remove wrinkles using Photoshop.You can easily remove wrinkles under eyes and others parts of face. I am going to show how to remove wrinkle from Jackie Chan: China's Top Celebrity. Lets now reduce Jackie's age. 1. Open a wrinkle face image. 2. Next … [Read more...]
Adobe Goes CS5
Everybody's favorite set of design tools always have photoshop as one of them,and all of adobe products which are collectively known as Creative Suite have also been the most recommended in field of graphics and design.Adobe now is releasing yet another improvement to their already powerful tools.Creative Suite 5.There is less than 2 days for the launch event and everyone are … [Read more...]


text:
Purchasing education is crucial to our region s economic health
After checking out the May 22 Forum short articles The Pittsburgh Region Will Need More Workers and STEM Still Stands Out, followed by Marty Kaplan s letter to the editor, Gov. Wolf Values Votes Over Education Quality, I feel there's a need to link the dots.
The Allegheny Conference on Community Developments Inflection Point: Supply, Demand and the Future of Work in the Pittsburgh Region highlights a looming gap in an extremely competent labor force needed to fill InfoTech, company and finance, health care, energy and advanced manufacturing tasks Click here.

subset: commoncrawl


text:
In a message dated 6/1/06 10:04:31 PM, email@example.com writes:
> I look for healthy, small
> plants that will more easily adapt to my location
Cathy, I, too, prefer smaller plants. I can tell you we are in the minority
regardless if it is a shrub, tree, perennial or annual and if it is a
bloomer, the more blooms the better. It's that demand for instant
To sign-off this list, send email to email@example.com with the
message text UNSUBSCRIBE GARDENCHAT


text:
Review all of the information we have provided for the clinics. Some of them provide a wide array of services ranging from free to sliding scale services. We have provided as much detailed information including phone numbers, emails, and websites where available.
Clinic services we list include:
Medical Clinic Services
Women's Health Clinic Services
Adult Health Clinic Services
Pediatric Dental Clinic Services
Behavioral Health Clinic Services
and more!!
Your feedback will help us improve our website!
No listings were found in Woodbridgeship.
If you wish to contribute to our database, please join now and add a listing.
We have listed some other listings in New Jersey below. We hope they are helpful in your free clinic search. 222
West New York Office-Universal Dental - West New York
(201) 868-6400
Sliding Scale
Services: Cleanings & Prevention Dental Exams & Cleanings Dental X-Rays Home Care How to Properly Brush & Floss Cosmetic Dentistry Composite Fillings & see clinic details
Cooper University Health Care- Audubon 2 - Audubon
(856) 874-0134
Sliding Scale
Urgent Care Center see clinic details
Cooper University Health Care- Audubon - Audubon
(856) 547-8415
Sliding Scale
Services:Internal Family and Community Medicine see clinic details
Cooper University Health Care- Camden - Camden
(856) 342-2000
Sliding Scale
Department(s) and programs at this location: Adolescent Medicine Suite 200 (856) 342-2001Audiology 511 856. 342.3113Breast Imaging Centers Suite 100 (888) see clinic details
CompleteCare Adult & Specialty Medical Professionals - Vineland - Vineland
(856) 451-4700
Sliding Scale
Services Offered:• Adult Primary Care• Infectious Disease• Nephrology• Eye Care• Cardiology• GynecologyThis Health Center is based on Sliding scale fees which are variable prices for products, services, or taxes based on a customer's ability to pay. Such fees are thereby reduced for thos see clinic details
Community Health Care Smile Center - Bridgeton
(856) 451-4700
Sliding Scale
Services Offered:• Dental CareSpecializing In:• Examinations• X-rays• Restorative and Preventive Dentistry• Cleaning and Dentures• Sealants and Fluoride Application• Routine dental surgery This Health Center is based on Sliding scale fees which are variable prices for products, service see clinic details
As an enhancement to our free medical clinic listings we now provide sliding scale treatment clinics. Sliding fee scale are variable prices for services due to customers ability to pay. Medical and dental fees are reduced due to many factors. That is why you need to call the clinic directly on these sliding fee scale clinics.
We also provide income based clinics. These clinics are HRSA Health Centers. You pay what you can afford based on your income.
Obamacare Open Enrollment is now. Sign up for savings!Best ObamaCare
Are You Looking For Free Dental Clinics In New Jersey?New Jersey Free Dental Clinics
Your Simple Guide to Losing 20 Pounds
The trend in our culture is toward gaining a pound or two every year. This is one trend that you shouldn't follow. You can easily stop this trend and maintain your weight by cutting a mere 100 calories from your diet each day. If you want to rever
Lower Blood Pressure Naturally
High blood pressure, also known as hypertension, is often the underlying cause of a heart attack or stroke in people as young as forty. Both of these things in the top three causes of death in the United States. Anyone who is diagnosed with hypert
How to Naturally Lower Cholesterol
High cholesterol levels can cause cardiovascular disease, heart attack or strokes. There are several ways you can help reduce your LDL (bad) cholesterol and increase your HDL (good) cholesterol levels naturally.
Cholesterol is a waxy


text:
The Global FlyFisher
Simply the Best Place to go for Online Fly Fishing and Fly Tyinghttp://globalflyfisher.com/video/intruder-0?page=1
Simply the Best Place to go for Online Fly Fishing and Fly Tying
Nov 13. 2016
(1 replies)
Sep 12. 2016
(1 replies)
Apr 11. 2016
(1 replies)
Jul 12. 2015
(0 replies)
Jan 9. 2017
Thank you for the post
Nov 15. 2016
Moreno, Thanks for sharing. A great and...
Sep 28. 2016
Mike, Your questions are not stupid at...
Apr 17. 2016
Tim, What a good looking style! I love...
Published May 4. 2013 - 3 years ago
Updated or edited Feb 22. 2016
Related articles
I know I know, there is not a shortage of tying videos about this style of fly. I wanted to do a video on these flies just because of how popular they are. Of course this is my version of this fly, and there are MANY ways of tying an Intruder. I hope you enjoy my rendition. Thanks! Continued in part 2.
Originator's web site:
Martin Joergensen
Add new comment
Log in or register to pre-fill name on comments, add videos, user pictures and more.
Read more about why you should register.
Read more about why you should register.
Related content
Based on these keywords | Intruders |
ContactContact us
Contribute articles
No guest posts
The Global FlyFisher Newsletter
About the Global FlyFisher
Discussion forum
PoliciesCopyright © 1994-2017
Copyright © The Global FlyFisher
Copyright © contributing authors
No reprint without permission
- paper, electronic or otherwise
Copyright policy
Review policy
More policies


text:
Clinical Focus
- Anatomic Pathology
- Molecular Pathology
Education & Certifications
Board Certification: Molecular Genetic Pathology, American Board of Pathology (2013)
Fellowship:Stanford University School of Medicine (2013) CA
Fellowship:Stanford Hospital and Clinics (2012) CA
Residency:Stanford Hospital and Clinics (2011) CA
Medical Education:Stanford University School of Medicine (2009) CA
Board Certification: Anatomic Pathology, American Board of Pathology (2012)
Additional Info
All Publications
Next-Generation Molecular Testing of Newborn Dried Blood Spots for Cystic Fibrosis
2016; 18 (2): 267-282
Newborn screening for cystic fibrosis enables early detection and management of this debilitating genetic disease. Implementing comprehensive CFTR analysis using Sanger sequencing as a component of confirmatory testing of all screen-positive newborns has remained impractical due to relatively lengthy turnaround times and high cost. Here, we describe CFseq, a highly sensitive, specific, rapid (<3 days), and cost-effective assay for comprehensive CFTR gene analysis from dried blood spots, the common newborn screening specimen. The unique design of CFseq integrates optimized dried blood spot sample processing, a novel multiplex amplification method from as little as 1 ng of genomic DNA, and multiplex next-generation sequencing of 96 samples in a single run to detect all relevant CFTR mutation types. Sequence data analysis utilizes publicly available software supplemented by an expert-curated compendium of >2000 CFTR variants. Validation studies across 190 dried blood spots demonstrated 100% sensitivity and a positive predictive value of 100% for single-nucleotide variants and insertions and deletions and complete concordance across the polymorphic poly-TG and consecutive poly-T tracts. Additionally, we accurately detected both a known exon 2,3 deletion and a previously undetected exon 22,23 deletion. CFseq is thus able to replace all existing CFTR molecular assays with a single robust, definitive assay at significant cost and time savings and could be adapted to high-throughput screening of other inherited conditions.
View details for DOI 10.1016/j.jmoldx.2015.11.005
View details for Web of Science ID 000370997200013
View details for PubMedID 26847993
Rapid, High-Quality, Cost-Effective, Comprehensive and Expandable Targeted Next-Generation Sequencing Assay for Inherited Heart Diseases
2015; 117 (7): 603-611
Thousands of mutations across more than 50 genes have been implicated in inherited cardiomyopathies. However, options for sequencing this rapidly evolving gene set are limited as many sequencing services and off-the-shelf kits suffer from slow turnaround, inefficient capture of genomic DNA, and/or high cost. Furthermore, customization of these assays to cover emerging targets and to suit individual needs is often expensive and time-consuming.We sought to develop a custom high throughput, clinical-grade next generation sequencing (NGS) assay for detecting cardiac disease gene mutations with improved accuracy, flexibility, turnaround, and cost.We employed double-stranded probes (complementary long "padlock" probes (cLPPs)), an inexpensive and customizable capture technology, to efficiently capture and amplify the entire coding region and flanking intronic and regulatory sequences of 88 genes and 40 microRNAs (miRNA) associated with inherited cardiomyopathies, congenital heart disease (CHD), and cardiac development. Multiplexing 11 samples per sequencing run resulted in a mean base coverage of 420, of which 97% had >20X coverage and >99% were concordant with known heterozygous single nucleotide polymorphisms (SNPs). The assay correctly detected germline variants in 24 individuals and revealed several polymorphic regions in miR-499. Total run time was three days at an approximate cost of $100 per sample.Accurate, high throughput detection of mutations across numerous cardiac genes is achievable with cLPP technology. Moreover, this format allows facile insertion of additional probes as more cardiomyopathy and CHD genes are discovered, giving researchers a powerful new tool for DNA mutation detection and discovery.
View details for DOI 10.1161/CIRCRESAHA.115.306723
View details for Web of Science ID 000360967000006
Alterations in DNA methylation may be the key to early detection and treatment of schistosomal bladder cancer.
PLoS neglected tropical diseases
2015; 9 (6)
View details for DOI 10.1371/journal.pntd.0003696
View details for PubMedID 26042665
Macrophages are required for host survival in experimental urogenital schistosomiasis.
FASEB journal
2015; 29 (1): 193-207
Urogenital schistosomiasis, Schistosoma haematobium worm infection, afflicts millions of people with egg-triggered, fibrotic bladder granulomata. Despite the significant global impact of urogenital schistosomiasis, the mechanisms of bladder granulomogenesis and fibrosis are ill defined due to the prior lack of tractable animal models. We combined a mouse model of urogenital schistosomiasis with macrophage-depleting liposomal clodronate (LC) to define how macrophages mediate bladder granulomogenesis and fibrosis. Mice were injected with eggs purified from infected hamsters or vehicle prepared from uninfected hamster tissues (xenoantigen and injection trauma control). Empty liposomes were controls for LC: 1) LC treatment resulted in fewer bladder egg granuloma-infiltrating macrophages, eosinophils, and T and B cells, lower bladder and serum levels of eotaxin, and higher bladder concentrations of IL-1α and chemokines (in a time-dependent fashion), confirming that macrophages orchestrate leukocyte infiltration of the egg-exposed bladder; 2) macrophage-depleted mice exhibited greater weight loss and bladder hemorrhage postegg injection; 3) early LC treatment postegg injection resulted in profound decreases in bladder fibrosis, suggesting differing roles for macrophages in fibrosis over time; and 4) LC treatment also led to egg dose-dependent mortality, indicating that macrophages prevent death from urogenital schistosomiasis. Thus, macrophages are a potential therapeutic target for preventing or treating the bladder sequelae of urogenital schistosomiasis.-Fu, C.-L., Odegaard, J. I., Hsieh, M. H. Macrophages are required for host survival in experimental urogenital schistosomiasis.
View details for DOI 10.1096/fj.14-259572
View details for PubMedID 25351984
Identification of Orai1 Channel Inhibitors by Using Minimal Functional Domains to Screen Small Molecule Microarrays
2014; 21 (10): 1278-1292
View details for DOI 10.1016/j.chembiol.2014.08.016
View details for Web of Science ID 000344521300007
Immune responses to Schistosoma haematobium infection
2014; 36 (9): 428-438
View details for DOI 10.1111/pim.12084
View details for Web of Science ID 000341881900005
Identification of recurrent SMO and BRAF mutations in ameloblastomas
2014; 46 (7): 722-725
View details for DOI 10.1038/ng.2986
View details for Web of Science ID 000338093800013
Identification of recurrent SMO and BRAF mutations in ameloblastomas.
Nature genetics
2014; 46 (7): 722-725
Here we report the discovery of oncogenic mutations in the Hedgehog and mitogen-activated protein kinase (MAPK) pathways in over 80% of ameloblastomas, locally destructive odontogenic tumors of the jaw, by genomic analysis of archival material. Mutations in SMO (encoding Smoothened, SMO) are common in ameloblastomas of the maxilla, whereas BRAF mutations are predominant in tumors of the mandible. We show that a frequently occurring SMO alteration encoding p.Leu412Phe is an activating mutation and that its effect on Hedgehog-pathway activity can be inhibited by arsenic trioxide (ATO), an anti-leukemia drug approved by the US Food and Drug Administration (FDA) that is currently in clinical trials for its Hedgehog-inhibitory activity. In a similar manner, ameloblastoma cells harboring an activating BRAF mutation encoding p.Val600Glu are sensitive to the BRAF inhibitor vemurafenib. Our findings establish a new paradigm for the diagnostic classification and treatment of ameloblastomas.
View details for DOI 10.1038/ng.2986
View details for PubMedID 24859340
A new mouse model for female genital schistosomiasis.
PLoS neglected tropical diseases
2014; 8 (5)
Over 112 million people worldwide are infected with Schistosoma haematobium, one of the most prevalent schistosome species affecting humans. Female genital schistosomiasis (FGS) occurs when S. haematobium eggs are deposited into the female reproductive tract by adult worms, which can lead to pelvic pain, vaginal bleeding, genital disfigurement and infertility. Recent evidence suggests co-infection with S. haematobium increases the risks of contracting sexually transmitted diseases such as HIV. The associated mechanisms remain unclear due to the lack of a tractable animal model. We sought to create a mouse model conducive to the study of immune modulation and genitourinary changes that occur with FGS.To model FGS in mice, we injected S. haematobium eggs into the posterior vaginal walls of 30 female BALB/c mice. A control group of 20 female BALB/c mice were injected with uninfected LVG hamster tissue extract. Histology, flow cytometry and serum cytokine levels were assessed at 2, 4, 6, and 8 weeks post egg injection. Voiding studies were performed at 1 week post egg injection.Vaginal wall injection with S. haematobium eggs resulted in synchronous vaginal granuloma development within 2 weeks post-egg injection that persisted for at least 6 additional weeks. Flow cytometric analysis of vaginal granulomata revealed infiltration by CD4+ T cells with variable expression of the HIV co-receptors CXCR4 and CCR5. Granulomata also contained CD11b+F4/80+ cells (macrophages and eosinophils) as well as CXCR4+MerTK+ macrophages. Strikingly, vaginal wall-injected mice featured significant urinary frequency despite the posterior vagina being anatomically distant from the bladder. This may represent a previously unrecognized overactive bladder response to deposition of schistosome eggs in the vagina.We have established a new mouse model that could potentially enable novel studies of genital schistosomiasis in females. Ongoing studies will further explore the mechanisms by which HIV target cells may be drawn into FGS-associated vaginal granulomata.
View details for DOI 10.1371/journal.pntd.0002825
View details for PubMedID 24786606
Cellular origin of bladder neoplasia and tissue dynamics of its progression to invasive carcinoma
View details for DOI 10.1038/ncb2956
View details for Web of Science ID 000335438400011
A New Mouse Model for Female Genital Schistosomiasis
2014; 8 (5)
View details for DOI 10.1371/journal.pntd.0002825
View details for Web of Science ID 000337735100024
Cellular origin of bladder neoplasia and tissue dynamics of its progression to invasive carcinoma.
Nature cell biology
2014; 16 (5): 469-478
Understanding how malignancies arise within normal tissues requires identification of the cancer cell of origin and knowledge of the cellular and tissue dynamics of tumour progression. Here we examine bladder cancer in a chemical carcinogenesis model that mimics muscle-invasive human bladder cancer. With no prior bias regarding genetic pathways or cell types, we prospectively mark or ablate cells to show that muscle-invasive bladder carcinomas arise exclusively from Sonic hedgehog (Shh)-expressing stem cells in basal urothelium. These carcinomas arise clonally from a single cell whose progeny aggressively colonize a major portion of the urothelium to generate a lesion with histological features identical to human carcinoma in situ. Shh-expressing basal cells within this precursor lesion become tumour-initiating cells, although Shh expression is lost in subsequent carcinomas. We thus find that invasive carcinoma is initiated from basal urothelial stem cells but that tumour cell phenotype can diverge significantly from that of the cancer cell of origin.
View details for DOI 10.1038/ncb2956
View details for PubMedID 24747439
Transplanted terminally differentiated induced pluripotent stem cells are accepted by immune mechanisms similar to self-tolerance
2014; 5
View details for DOI 10.1038/ncomms4903
View details for Web of Science ID 000337504100006
Transplanted terminally differentiated induced pluripotent stem cells are accepted by immune mechanisms similar to self-tolerance.
Nature communications
2014; 5: 3903-?
The exact nature of the immune response elicited by autologous-induced pluripotent stem cell (iPSC) progeny is still not well understood. Here we show in murine models that autologous iPSC-derived endothelial cells (iECs) elicit an immune response that resembles the one against a comparable somatic cell, the aortic endothelial cell (AEC). These cells exhibit long-term survival in vivo and prompt a tolerogenic immune response characterized by elevated IL-10 expression. In contrast, undifferentiated iPSCs elicit a very different immune response with high lymphocytic infiltration and elevated IFN-γ, granzyme-B and perforin intragraft. Furthermore, the clonal structure of infiltrating T cells from iEC grafts is statistically indistinguishable from that of AECs, but is different from that of undifferentiated iPSC grafts. Taken together, our results indicate that the differentiation of iPSCs results in a loss of immunogenicity and leads to the induction of tolerance, despite expected antigen expression differences between iPSC-derived versus original somatic cells.
View details for DOI 10.1038/ncomms4903
View details for PubMedID 24875164
Aortic wall thickness: an independent risk factor for aortic dissection?
journal of heart valve disease
2014; 23 (1): 17-24
Aortic aneurysm size is known to portend a higher likelihood of aortic complications in patients with connective tissue disorders (CTD), but other objective tools are needed to determine which patients are at greatest risk of dissection, especially those which reflect the structural integrity and strength of the aortic wall.The aortic wall pathology was evaluated in CTD patients with and without acute aortic dissection to identify parameters that affect the risk of dissection. A retrospective review was performed of aneurysm pathology from patients with Marfan syndrome (MFS; n = 53) without dissection undergoing prophylactic aortic root surgery, and acute type A aortic dissection patients (AAAoD; n = 16). Patients without a cardiovascular cause of death (n = 19) served as controls. The minimal aortic medial wall thickness was measured, and medial myxoid degeneration (MMD) and the degree of elastin loss and fragmentation were graded.The mean minimal aortic wall thickness was 1,625 +/- 364 microm in controls, and 703 +/- 256 microm and 438 +/- 322 microm for MFS and AAAoD patients, respectively. Aortic root diameters did not correlate with aortic wall thickness. A comparison of aortic medial thickness showed that the media was significantly thinner among acute dissection patients than either elective surgical patients (p = 0.02) or controls (p < 0.001). Aortic size, degree of MMD, and elastin loss did not vary significantly between CTD patients.A diminished aortic wall medial thickness may be linked to aortic dissection. High-resolution imaging techniques in the future may lead to the morphological assessment of aortic medial wall thickness in vivo becoming a reality which, in theory, could provide a more refined risk prognostication for acute aortic dissection.
View details for PubMedID 24779324
Costimulation-Adhesion Blockade Is Superior to Cyclosporine A and Prednisone Immunosuppressive Therapy for Preventing Rejection of Differentiated Human Embryonic Stem Cells Following Transplantation
2013; 31 (11): 2354-2363
Rationale: Human embryonic stem cell (hESC) derivatives are attractive candidates for therapeutic use. The engraftment and survival of hESC derivatives as xenografts or allografts require effective immunosuppression to prevent immune cell infiltration and graft destruction. Objective: To test the hypothesis that a short-course, dual-agent regimen of two costimulation-adhesion blockade agents can induce better engraftment of hESC derivatives compared to current immunosuppressive agents. Methods and Results: We transduced hESCs with a double fusion reporter gene construct expressing firefly luciferase (Fluc) and enhanced green fluorescent protein, and differentiated these cells to endothelial cells (hESC-ECs). Reporter gene expression enabled longitudinal assessment of cell engraftment by bioluminescence imaging. Costimulation-adhesion therapy resulted in superior hESC-EC and mouse EC engraftment compared to cyclosporine therapy in a hind limb model. Costimulation-adhesion therapy also promoted robust hESC-EC and hESC-derived cardiomyocyte survival in an ischemic myocardial injury model. Improved hESC-EC engraftment had a cardioprotective effect after myocardial injury, as assessed by magnetic resonance imaging. Mechanistically, costimulation-adhesion therapy is associated with systemic and intragraft upregulation of T-cell immunoglobulin and mucin domain 3 (TIM3) and a reduced proinflammatory cytokine profile. Conclusions: Costimulation-adhesion therapy is a superior alternative to current clinical immunosuppressive strategies for preventing the post-transplant rejection of hESC derivatives. By extending the window for cellular engraftment, costimulation-adhesion therapy enhances functional preservation following ischemic injury. This regimen may function through a TIM3-dependent mechanism. Stem Cells 2013;31:2354-2363.
View details for DOI 10.1002/stem.1501
View details for Web of Science ID 000327025600007
Excimer Laser-Assisted Removal of Embedded Inferior Vena Cava Filters A Single-Center Prospective Study
2013; 6 (5): 560-566
Although chronically implanted inferior vena cava filters may result in filter-related morbidity, there is currently no routine option for removing such filters when they become firmly embedded along the vena cava endothelium.During a 3-year period, 100 consecutive patients were prospectively enrolled in a single-center study. There were 42 men and 58 women (mean age, 46 years; limits, 18-76 years). Retrieval indications included filter-related acute inferior vena cava thrombosis, chronic inferior vena cava occlusion, and pain from retroperitoneal or bowel penetration. Filter retrieval was also performed to prevent risks from prolonged implantation and to potentially eliminate the need for lifelong anticoagulation. After standard methods failed, photothermal tissue ablation was attempted with a laser sheath powered by a 308-nm xenon chloride excimer laser. Applied forces were recorded with a digital tension meter before and during laser activation. Laser-assisted retrieval was successful in 98.0% (95% confidence interval [CI], 93.0%-99.8%) with mean implantation of 855 days (limits, 37-6663 days; >18 years). The following filter types were encountered in this study: Günther-Tulip (n=34), Celect (n=12), Option (n=17), Optease (n=20, 1 failure), TrapEase (n=6, 1 failure), Simon-Nitinol (n=1), 12F Stainless Steel Greenfield (n=4), and Titanium Greenfield (n=6). The average force during failed standard retrievals was 7.2 versus 4.6 pounds during laser-assisted retrievals (P<0.0001). The major complication rate was 3.0% (95% CI, 0.6%-8.5%), the minor complication rate was 7.0% (95% CI, 0.3%-13.9%), and there were 4 adverse events (2 coagulopathic hemorrhages, 1 renal infarction, and 1 cholecystitis; 4.0%; 95% CI, 1.1%-9.9%) at mean follow-up of 500 days (limits, 84-1079 days). Scar tissue ablation was histologically confirmed in 96.0% (95% CI, 89.9%-98.9%). Successful retrieval allowed cessation of anticoagulation in 30 of 30 (100%) patients and alleviated morbidity in 23 of 24 patients (96%).Excimer laser-assisted removal is effective in removing embedded inferior vena cava filters refractory to standard retrieval and high force. This method can be safely used to prevent and alleviate filter-related morbidity.http://www.clinicaltrials.gov. Unique identifier: NCT01158482.
View details for DOI 10.1161/CIRCINTERVENTIONS.113.000665
View details for Web of Science ID 000329923100014
View details for PubMedID 24065445
Eosinophils secrete IL-4 to facilitate liver regeneration.
Proceedings of the National Academy of Sciences of the United States of America
2013; 110 (24): 9914-9919
The liver is a central organ for the synthesis and storage of nutrients, production of serum proteins and hormones, and breakdown of toxins and metabolites. Because the liver is susceptible to toxin- or pathogen-mediated injury, it maintains a remarkable capacity to regenerate by compensatory growth. Specifically, in response to injury, quiescent hepatocytes enter the cell cycle and undergo DNA replication to promote liver regrowth. Despite the elucidation of a number of regenerative factors, the mechanisms by which liver injury triggers hepatocyte proliferation are incompletely understood. We demonstrate here that eosinophils stimulate liver regeneration after partial hepatectomy and toxin-mediated injury. Liver injury results in rapid recruitment of eosinophils, which secrete IL-4 to promote the proliferation of quiescent hepatocytes. Surprisingly, signaling via the IL-4Rα in macrophages, which have been implicated in tissue repair, is dispensable for hepatocyte proliferation and liver regrowth after injury. Instead, IL-4 exerts its proliferative actions via IL-4Rα in hepatocytes. Our findings thus provide a unique mechanism by which eosinophil-derived IL-4 stimulates hepatocyte proliferation in regenerating liver.
View details for DOI 10.1073/pnas.1304046110
View details for PubMedID 23716700
Complex retrieval of fractured, embedded, and penetrating inferior vena cava filters: a prospective study with histologic and electron microscopic analysis.
Journal of vascular and interventional radiology
2013; 24 (5): 622-630 e1
To evaluate clinical outcomes, characterize adherent tissue, and analyze inferior vena cava (IVC) filter fractures in patients undergoing complex retrieval for management of filter-related complications. To elucidate mechanisms of filter fracture by radiographic and electron microscopic (EM) evaluation.Over 2.5 years, 50 consecutive patients with fractured and/or penetrating filter components were prospectively enrolled into a single-center study. There were 19 men and 31 women (mean age, 42 y; range, 15-73 y). All patients underwent complex filter retrieval after failure of standard methods, and retrieval indications along with resultant clinical outcomes were evaluated. Specimens with adherent tissue underwent histologic analysis, and all fractured components were studied with EM.Retrieval was successful in all 50 cases (mean implantation, 815 d; range, 20-2,599 d) among the following filters: G2X (n = 23),G2 (n = 9), Eclipse (n = 3), Recovery (n = 4), ALN (n = 1), Celect (n = 7), OptEase (n = 2), and Simon Nitinol (n = 1). Mean indwell time in fractured filters (n = 31) was 1,082 days, versus 408 days in nonfractured filters (n = 19; P = .00169). Neointimal hyperplasia/fibrosis was seen in 46 of 48 specimens with adherent tissue (96%). Among 61 fractured components from conical filters, 35 had extravascular penetration whereas 26 remained intravascular (11 free-floating in IVC, 15 embolized centrally), and EM revealed fracture modes of high-cycle fatigue (n = 53), overload (n = 6), and indeterminate (n = 2). Following retrieval, previously prescribed lifelong anticoagulation was discontinued in 30 of 31 patients (97%). Filter-related symptoms from IVC occlusion, component embolization, and penetration-induced abdominal pain, duodenal injury, and/or small-bowel volvulus were alleviated in all 26 cases (100%). There were no long-term complications at a mean follow-up of 371 days (range, 67-878 d).The risk of filter fracture increases after 408 days (ie,>1 y) of implantation and is associated with symptomatic extravascular penetration and/or intravascular embolization. Complex methods can be used to safely remove these devices, alleviate filter-related morbidity, and allow cessation of anticoagulation.
View details for DOI 10.1016/j.jvir.2013.01.008
View details for PubMedID 23523157
Complex Retrieval of Fractured, Embedded, and Penetrating Inferior Vena Cava Filters: A Prospective Study with Histologic and Electron Microscopic Analysis
2013; 24 (5): 622-630
View details for DOI 10.1016/j.jvir.2013.01.008
View details for Web of Science ID 000318263500002
The immune system as a sensor of the metabolic state.
2013; 38 (4): 644-654
Mammals possess a remarkable ability to maintain and defend a constant internal milieu against diverse environmental threats. Unsurprisingly, the two systems tasked with these duties, metabolism and immunity, have evolved to share a common modular architecture that allows extensive bidirectional communication and coordination. Indeed, recent observations have highlighted numerous functionally critical immune regulatory modules located within diverse metabolic circuits. In this review, we discuss the architectural commonality between immunity and metabolism and highlight how these two primordially disparate systems leverage shared regulatory axes to coordinate metabolic physiology under conditions of normality and chronic overnutrition. Such an integrated perspective both advances our understanding of basic physiology and highlights potential opportunities for therapeutic intervention in metabolic dysfunction.
View details for DOI 10.1016/j.immuni.2013.04.001
View details for PubMedID 23601683
Pleiotropic Actions of Insulin Resistance and Inflammation in Metabolic Homeostasis
2013; 339 (6116): 172-177
Metabolism and immunity are inextricably linked both to each other and to organism-wide function, allowing mammals to adapt to changes in their internal and external environments. In the modern context of obesogenic diets and lifestyles, however, these adaptive responses can have deleterious consequences. In this Review, we discuss the pleiotropic actions of inflammation and insulin resistance in metabolic homeostasis and disease. An appreciation of the adaptive context in which these responses arose is useful for understanding their pathogenic actions in disease.
View details for DOI 10.1126/science.1230721
View details for Web of Science ID 000313328200036
View details for PubMedID 23307735
Adipose Tissue Macrophages: Amicus adipem?
Cell metabolism
2013; 18 (6): 767-8
Chronic overnutrition drives complex adaptations within both professional metabolic and bystander tissues that, despite intense investigation, are still poorly understood. Xu et al. (2013) now describe the unexpected ability of adipose tissue macrophages to buffer lipids released from obese adipocytes in a manner independent of inflammatory macrophage activation.
View details for DOI 10.1016/j.cmet.2013.11.011
View details for PubMedID 24315364
Fat's Immune Sentinels
2012; 26 (12): 30-35
View details for DOI 10.3410/B4-13
View details for Web of Science ID 000312240200009
Transcriptional Profiling of the Bladder in Urogenital Schistosomiasis Reveals Pathways of Inflammatory Fibrosis and Urothelial Compromise
2012; 6 (11)
Urogenital schistosomiasis, chronic infection by Schistosoma haematobium, affects 112 million people worldwide. S. haematobium worm oviposition in the bladder wall leads to granulomatous inflammation, fibrosis, and egg expulsion into the urine. Despite the global impact of urogenital schistosomiasis, basic understanding of the associated pathologic mechanisms has been incomplete due to the lack of suitable animal models. We leveraged our recently developed mouse model of urogenital schistosomiasis to perform the first-ever profiling of the early molecular events that occur in the bladder in response to the introduction of S. haematobium eggs. Microarray analysis of bladders revealed rapid, differential transcription of large numbers of genes, peaking three weeks post-egg administration. Many differentially transcribed genes were related to the canonical Type 2 anti-schistosomal immune response, as reflected by the development of egg-based bladder granulomata. Numerous collagen and metalloproteinase genes were differentially transcribed over time, revealing complex remodeling and fibrosis of the bladder that was confirmed by Masson's Trichrome staining. Multiple genes implicated in carcinogenesis pathways, including vascular endothelial growth factor-, oncogene-, and mammary tumor-related genes, were differentially transcribed in egg-injected bladders. Surprisingly, junctional adhesion molecule, claudin and uroplakin genes, key components for maintaining the urothelial barrier, were globally suppressed after bladder exposure to eggs. This occurred in the setting of urothelial hyperplasia and egg shedding in urine. Thus, S. haematobium egg expulsion is associated with intricate modulation of the urothelial barrier on the cellular and molecular level. Taken together, our findings have important implications for understanding host-parasite interactions and carcinogenesis in urogenital schistosomiasis, and may provide clues for novel therapeutic strategies.
View details for DOI 10.1371/journal.pntd.0001912
View details for Web of Science ID 000311888900039
View details for PubMedID 23209855
Comparison of the acute inflammatory response of two commercial platelet-rich plasma systems in healthy rabbit tendons.
American journal of sports medicine
2012; 40 (6): 1274-1281
Numerous studies have shown platelet-rich plasma (PRP) preparations differ with respect to the inclusion of certain blood components, which may affect the host's cellular response.This study evaluated the inflammatory effect of Biomet GPS III leukocyte-rich PRP (LR-PRP) versus MTF Cascade leukocyte-poor PRP (LP-PRP) after intratendinous injection in an animal model. The authors anticipated that LR-PRP would incite a greater acute inflammatory response than LP-PRP.Controlled laboratory study.A total of 17 skeletally mature New Zealand White rabbits were tested. In all cases, healthy patellar tendons were treated. In the control animals, one patellar tendon was injected with 2 mL autologous whole blood, and the other was injected with 2 mL sterile saline. Seven total tendons were injected with whole blood, and 7 tendons were injected with saline. In the experimental animals, one patellar tendon was injected with 2 mL LR-PRP, and the other was injected with 2 mL LP-PRP. Ten tendons were injected with LR-PRP, and 10 tendons were injected with LP-PRP. Animals were euthanized at 5 or 14 days after injection. Tendons were harvested and stained using hematoxylin and eosin and scored semi-quantitatively for total white blood cells (WBCs), mononuclear cells (macrophages and lymphocytes), polymorphonuclear cells (PMNs), vascularity, fiber structure, and fibrosis.At 5 days after injection, tendons treated with LR-PRP had significantly greater overall tendon scores (6.3 ± 1.79 vs 1.8 ± 1.64, P = .012), as well as mean scores for fiber structure (1.4 ± 0.22 vs 0.50 ± 0.50, P = .012), denoting disrupted composition, total WBCs (1.1 ± 0.89 vs 0.10 ± 0.22, P = .014), mononuclear cells (macrophages and lymphocytes) (0.80 ± 0.45 vs 0.10 ± 0.22, P = .014), vascularity (1.7 ± 0.27 vs 0.80 ± 0.16, P = .008), and fibrosis (1.0 ± 0.35 vs 0.3 ± 0.45, P = .037) compared with tendons treated with LP-PRP. Otherwise, there were no significant differences in mononuclear cells (P = .590), PMN cells (P = 1.00), total WBCs (P = .811), vascularity (P = .650), or total tendon score (P = .596) in any of the treatment groups at 14 days.Compared with leukocyte-poor Cascade PRP, leukocyte-rich GPS III PRP causes a significantly greater acute inflammatory response at 5 days after injection. There is no significant difference in the inflammatory response or cellularity regardless of the injection type at 14 days after intratendinous injection.Platelet-rich plasma injections are frequently prepared using commercial systems and are administered for clinical treatment of chronic tendinopathy. It is important to characterize the cellular responses elucidated by different injection preparations to further understand their effect on tissue healing and aid clinical decision making. Future investigations are necessary to apply these findings to the clinical setting.
View details for DOI 10.1177/0363546512442334
View details for PubMedID 22495144
Connecting Type 1 and Type 2 Diabetes through Innate Immunity
2012; 4 (3)
The escalating epidemic of obesity has driven the prevalence of both type 1 and 2 diabetes mellitus to historically high levels. Chronic low-grade inflammation, which is present in both type 1 and type 2 diabetics, contributes to the pathogenesis of insulin resistance. The accumulation of activated innate immune cells in metabolic tissues results in release of inflammatory mediators, in particular, IL-1β and TNFα, which promote systemic insulin resistance and β-cell damage. In this article, we discuss the central role of innate immunity and, in particular, the macrophage in insulin sensitivity and resistance, β-cell damage, and autoimmune insulitis. We conclude with a discussion of the therapeutic implications of this integrated understanding of diabetic pathology.
View details for DOI 10.1101/cshperspect.a007724
View details for Web of Science ID 000300944200007
A Novel Mouse Model of Schistosoma haematobium Egg-Induced Immunopathology
2012; 8 (3)
Schistosoma haematobium is the etiologic agent for urogenital schistosomiasis, a major source of morbidity and mortality for more than 112 million people worldwide. Infection with S. haematobium results in a variety of immunopathologic sequelae caused by parasite oviposition within the urinary tract, which drives inflammation, hematuria, fibrosis, bladder dysfunction, and increased susceptibility to urothelial carcinoma. While humans readily develop urogenital schistosomiasis, the lack of an experimentally-tractable model has greatly impaired our understanding of the mechanisms that underlie this important disease. We have developed an improved mouse model of S. haematobium urinary tract infection that recapitulates several aspects of human urogenital schistosomiasis. Following microinjection of purified S. haematobium eggs into the bladder wall, mice consistently develop macrophage-rich granulomata that persist for at least 3 months and pass eggs in their urine. Importantly, egg-injected mice also develop urinary tract fibrosis, bladder dysfunction, and various urothelial changes morphologically reminiscent of human urogenital schistosomiasis. As expected, S. haematobium egg-induced immune responses in the immediate microenvironment, draining lymph nodes, and systemic circulation are associated with a Type 2-dominant inflammatory response, characterized by high levels of interleukin-4, eosinophils, and IgE. Taken together, our novel mouse model may help facilitate a better understanding of the unique pathophysiological mechanisms of epithelial dysfunction, tissue fibrosis, and oncogenesis associated with urogenital schistosomiasis.
View details for DOI 10.1371/journal.ppat.1002605
View details for Web of Science ID 000302225600051
View details for PubMedID 22479181
Bilateral Thoracic Endometriosis Affecting the Lung and Diaphragm
2012; 16 (1): 140-142
Endometriosis of the lung and the diaphragm is rare. Patients may present with symptoms such as shortness of breath, chest pain, and shoulder pain or they may be asymptomatic. Of note, there have been few reports of bilateral catamenial disease, and no reports, to our knowledge, of bilateral pathology proven pulmonary parenchymal endometriosis.A 43-year-old with stage IV endometriosis and large leiomyoma underwent a laparoscopic hysterectomy and treatment of endometrial lesions in 2005. In March and April of 2011, she presented with bilateral pneumothoraces. She subsequently underwent video-assisted thoracoscopy as well as resection and fulguration of bilateral lung and diaphragmatic endometriosis. Pathology confirmed endometrial implants in the lung parenchyma bilaterally.Catamenial pneumothorax is the most common presentation of thoracic endometriosis. However, bilateral catamenial pneumothoraces are rare. To the best of our knowledge, this case reflects the first report of pathology proven bilateral lung and diaphragm endometriosis.
View details for DOI 10.4293/108680812X13291597716384
View details for Web of Science ID 000307316100022
View details for PubMedID 22906342
Leukocyte set points in metabolic disease.
F1000 biology reports
2012; 4: 13-?
Vertebrate tissues comprise precise admixtures of parenchymal and hematopoietic cells, whose interactions are vital to proper tissue function. By regulating this interaction, vertebrates are able to mitigate environmental stress and coordinate dramatic physiologic adaptations. For instance, under conditions of chronic nutrient excess, leukocyte recruitment and activation increase in an effort to decrease excess nutrient storage and alleviate adipocyte stress. While basal equilibria may be reestablished upon normalization of nutrient intake, a new set point characterized by insulin resistance and chronic inflammation is established if the stress persists. Consequently, although this response is adaptive in settings of acute overfeeding and infection, it has catastrophic health consequences in the modern context of obesity. Understanding how leukocyte set points (numbers and activation status) are established, maintained, and regulated in tissues is, thus, critical to our understanding of, and intervention in, chronic metabolic diseases, such as obesity and diabetes.
View details for DOI 10.3410/B4-13
View details for PubMedID 22802874
Photothermal Ablation with the Excimer Laser Sheath Technique for Embedded Inferior Vena Cava Filter Removal: Initial Results from a Prospective Study
2011; 22 (6): 813-823
To evaluate the safety and effectiveness of the excimer laser sheath technique for removing embedded inferior vena cava (IVC) filters.Over 12 months, 25 consecutive patients undergoing attempted IVC filter retrieval with a laser-assisted sheath technique were prospectively enrolled into an institutional review board-approved study registry. There were 10 men and 15 women (mean age 50 years, range 20-76 years); 18 (72%) of 25 patients were referred from an outside hospital. Indications for retrieval included symptomatic filter-related acute caval thrombosis (with or without acute pulmonary embolism), chronic IVC occlusion, and bowel penetration. Retrieval was also performed to remove risks from prolonged implantation and potentially to eliminate need for lifelong anticoagulation. After failure of standard methods, controlled photothermal ablation of filter-adherent tissue with a Spectranetics laser sheath and CVX-300 laser system was performed. All patients were evaluated with cavography, and specimens were sent for histologic analysis.Laser-assisted retrieval was successful in 24 (96%) of 25 patients as follows: 11 Günther Tulip (mean 375 days, range 127-882 days), 4 Celect (mean 387 days, range 332-440 days), 2 Option (mean 215 days, range 100-330 days), 4 OPTEASE (mean 387 days, range 71-749 days; 1 failed 188 days), 2 TRAPEASE (mean 871 days, range 187-1,555 days), and 2 Greenfield (mean 12.8 years, range 7.2-18.3 years). There was one (4%) major complication (acute thrombus, treated with thrombolysis), three (12%) minor complications (small extravasation, self-limited), and one adverse event (coagulopathic retroperitoneal hemorrhage) at follow-up (mean 126 days, range 13-302 days). Photothermal ablation of filter-adherent tissue was histologically confirmed in 23 (92%) of 25 patients.The laser-assisted sheath technique appears to be a safe and effective tool for retrieving embedded IVC filters, including permanent types, with implantation ranging from months to > 18 years.
View details for DOI 10.1016/j.jvir.2011.01.459
View details for Web of Science ID 000291414500011
View details for PubMedID 21530309
Alternative Macrophage Activation and Metabolism
2011; 6: 275-297
Obesity and its attendant metabolic disorders represent the great public health challenge of our time. Recent evidence suggests that onset of inflammation in metabolic tissues pathogenically links obesity to insulin resistance and type 2 diabetes. In this review, we briefly summarize the extant literature, paying special attention to the central role of the tissue-associated macrophage in the initiation of metabolic inflammation. We argue that rather than representing simple inflammatory disease, obesity and metabolic syndrome represent derangements in macrophage activation with concomitant loss of metabolic coordination. As such, the sequelae of obesity are as much products of the loss of positive macrophage influences as they are of the presence of deleterious inflammation. The therapeutic implications of this conclusion are profound because they suggest that pharmacologic targeting of macrophage activation, rather than simply inflammation, might be efficacious in treating this global epidemic.
View details for DOI 10.1146/annurev-pathol-011110-130138
View details for Web of Science ID 000288922800012
View details for PubMedID 21034223
IL-4/STAT6 immune axis regulates peripheral nutrient metabolism and insulin sensitivity
2010; 107 (52): 22617-22622
Immune cells take residence in metabolic tissues, providing a framework for direct regulation of nutrient metabolism. Despite conservation of this anatomic relationship through evolution, the signals and mechanisms by which the immune system regulates nutrient homeostasis and insulin action remain poorly understood. Here, we demonstrate that the IL-4/STAT6 immune axis, a key pathway in helminth immunity and allergies, controls peripheral nutrient metabolism and insulin sensitivity. Disruption of signal transducer and activator of transcription 6 (STAT6) decreases insulin action and enhances a peroxisome proliferator-activated receptor ? (PPAR?) driven program of oxidative metabolism. Conversely, activation of STAT6 by IL-4 improves insulin action by inhibiting the PPAR?-regulated program of nutrient catabolism and attenuating adipose tissue inflammation. These findings have thus identified an unexpected molecular link between the immune system and macronutrient metabolism, suggesting perhaps the coevolution of these pathways occurred to ensure access to glucose during times of helminth infection.
View details for DOI 10.1073/pnas.1009152108
View details for Web of Science ID 000285684200051
View details for PubMedID 21149710
Prospective, Double-Blind, Randomized, Placebo-Controlled Comparison of Acetazolamide Versus Ibuprofen for Prophylaxis Against High Altitude Headache: The Headache Evaluation at Altitude Trial (HEAT)
2010; 21 (3): 236-243
High altitude headache (HAH) is the most common neurological complaint at altitude and the defining component of acute mountain sickness (AMS). However, there is a paucity of literature concerning its prevention. Toward this end, we initiated a prospective, double-blind, randomized, placebo-controlled trial in the Nepal Himalaya designed to compare the effectiveness of ibuprofen and acetazolamide for the prevention of HAH.Three hundred forty-three healthy western trekkers were recruited at altitudes of 4280 m and 4358 m and assigned to receive ibuprofen 600 mg, acetazolamide 85 mg, or placebo 3 times daily before continued ascent to 4928 m. Outcome measures included headache incidence and severity, AMS incidence and severity on the Lake Louise AMS Questionnaire (LLQ), and visual analog scale (VAS).Two hundred sixty-five of 343 subjects completed the trial. HAH incidence was similar when treated with acetazolamide (27.1%) or ibuprofen (27.5%; P = .95), and both agents were significantly more effective than placebo (45.3%; P = .01). AMS incidence was similar when treated with acetazolamide (18.8%) or ibuprofen (13.7%; P = .34), and both agents were significantly more effective than placebo (28.6%; P = .03). In fully compliant participants, moderate or severe headache incidence was similar when treated with acetazolamide (3.8%) or ibuprofen (4.7%; P = .79), and both agents were significantly more effective than placebo (13.5%; P = .03).Ibuprofen and acetazolamide were similarly effective in preventing HAH. Ibuprofen was similar to acetazolamide in preventing symptoms of AMS, an interesting finding that implies a potentially new approach to prevention of cerebral forms of acute altitude illness.
View details for Web of Science ID 000282163300007
View details for PubMedID 20832701
PPAR-delta senses and orchestrates clearance of apoptotic cells to promote tolerance
Macrophages rapidly engulf apoptotic cells to limit the release of noxious cellular contents and to restrict autoimmune responses against self antigens. Although factors participating in recognition and engulfment of apoptotic cells have been identified, the transcriptional basis for the sensing and the silent disposal of apoptotic cells is unknown. Here we show that peroxisome proliferator-activated receptor-delta (PPAR-delta) is induced when macrophages engulf apoptotic cells and functions as a transcriptional sensor of dying cells. Genetic deletion of PPAR-delta decreases expression of opsonins such as complement component-1qb (C1qb), resulting in impairment of apoptotic cell clearance and reduction in anti-inflammatory cytokine production. This increases autoantibody production and predisposes global and macrophage-specific Ppard(-/-) mice to autoimmune kidney disease, a phenotype resembling the human disease systemic lupus erythematosus. Thus, PPAR-delta has a pivotal role in orchestrating the timely disposal of apoptotic cells by macrophages, ensuring that tolerance to self is maintained.
View details for DOI 10.1038/nm.2048
View details for Web of Science ID 000271543700014
View details for PubMedID 19838202
Mechanisms of macrophage activation in obesity-induced insulin resistance
2008; 4 (11): 619-626
Chronic inflammation is now recognized as a key step in the pathogenesis of obesity-induced insulin resistance and type 2 diabetes mellitus. This low-grade inflammation is mediated by the inflammatory (classical) activation of recruited and resident macrophages that populate metabolic tissues, including adipose tissue and liver. These findings have led to the concept that infiltration by and activation of macrophages in adipose tissue are causally linked to obesity-induced insulin resistance. Studies have shown, however, that alternatively activated macrophages taking residence in adipose tissue and liver perform beneficial functions in obesity-induced metabolic disease. Alternatively activated macrophages reduce insulin resistance in obese mice by attenuating tissue inflammation and increasing oxidative metabolism in liver and skeletal muscle. The discovery that distinct subsets of macrophages are involved in the promotion or attenuation of insulin resistance suggests that pathways controlling macrophage activation can potentially be targeted to treat these comorbidities of obesity. Thus, this Review focuses on the stimuli and mechanisms that control classical and alternative activation of tissue macrophages, and how these macrophage activation programs modulate insulin action in peripheral tissues. The functional importance of macrophage activation is further discussed in the context of host defense to highlight the crosstalk between innate immunity and metabolism.
View details for DOI 10.1038/ncpendmet0976
View details for Web of Science ID 000260165700012
View details for PubMedID 18838972
Alternative M2 activation of Kupffer cells by PPAR delta ameliorates obesity-induced insulin resistance
2008; 7 (6): 496-507
Macrophage infiltration and activation in metabolic tissues underlie obesity-induced insulin resistance and type 2 diabetes. While inflammatory activation of resident hepatic macrophages potentiates insulin resistance, the functions of alternatively activated Kupffer cells in metabolic disease remain unknown. Here we show that in response to the Th2 cytokine interleukin-4 (IL-4), peroxisome proliferator-activated receptor delta (PPARdelta) directs expression of the alternative phenotype in Kupffer cells and adipose tissue macrophages of lean mice. However, adoptive transfer of PPARdelta(-/-) (Ppard(-/-)) bone marrow into wild-type mice diminishes alternative activation of hepatic macrophages, causing hepatic dysfunction and systemic insulin resistance. Suppression of hepatic oxidative metabolism is recapitulated by treatment of primary hepatocytes with conditioned medium from PPARdelta(-/-) macrophages, indicating direct involvement of Kupffer cells in liver lipid metabolism. Taken together, these data suggest an unexpected beneficial role for alternatively activated Kupffer cells in metabolic syndrome and type 2 diabetes.
View details for DOI 10.1016/j.cmet.2008.04.003
View details for Web of Science ID 000256443400007
View details for PubMedID 18522831
Macrophage-specific PPAR gamma controls alternative activation and improves insulin resistance
Obesity and insulin resistance, the cardinal features of metabolic syndrome, are closely associated with a state of low-grade inflammation. In adipose tissue chronic overnutrition leads to macrophage infiltration, resulting in local inflammation that potentiates insulin resistance. For instance, transgenic expression of Mcp1 (also known as chemokine ligand 2, Ccl2) in adipose tissue increases macrophage infiltration, inflammation and insulin resistance. Conversely, disruption of Mcp1 or its receptor Ccr2 impairs migration of macrophages into adipose tissue, thereby lowering adipose tissue inflammation and improving insulin sensitivity. These findings together suggest a correlation between macrophage content in adipose tissue and insulin resistance. However, resident macrophages in tissues display tremendous heterogeneity in their activities and functions, primarily reflecting their local metabolic and immune microenvironment. While Mcp1 directs recruitment of pro-inflammatory classically activated macrophages to sites of tissue damage, resident macrophages, such as those present in the adipose tissue of lean mice, display the alternatively activated phenotype. Despite their higher capacity to repair tissue, the precise role of alternatively activated macrophages in obesity-induced insulin resistance remains unknown. Using mice with macrophage-specific deletion of the peroxisome proliferator activated receptor-gamma (PPARgamma), we show here that PPARgamma is required for maturation of alternatively activated macrophages. Disruption of PPARgamma in myeloid cells impairs alternative macrophage activation, and predisposes these animals to development of diet-induced obesity, insulin resistance, and glucose intolerance. Furthermore, gene expression profiling revealed that downregulation of oxidative phosphorylation gene expression in skeletal muscle and liver leads to decreased insulin sensitivity in these tissues. Together, our findings suggest that resident alternatively activated macrophages have a beneficial role in regulating nutrient homeostasis and suggest that macrophage polarization towards the alternative state might be a useful strategy for treating type 2 diabetes.
View details for DOI 10.1038/nature05894
View details for Web of Science ID 000247564600038
View details for PubMedID 17515919
Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages
2007; 81 (3): 711-719
Macrophages participate in physiologic and pathologic processes through elaboration of distinct activation programs. Studies with macrophage cell systems have revealed much concerning the importance of this pleiotropic cell; however, these studies are inherently limited by three factors: heterogeneity of the target cell population, poor capacity to elaborate various activation programs, and lack of a genetically tractable model system for loss- and gain-of-function studies. Although definitive, hematopoietic lineages can be isolated from embryonic stem (ES) cells, these isolation procedures are inefficient and time-consuming and require elaborate cell-sorting protocols. We therefore examined whether myeloid precursors, capable of differentiating into macrophages, could be conditionally expanded in vitro. Here, we report methods for selective isolation and immortalization of ES cell-derived myeloid precursors by estrogen-regulated HoxA9 protein. Using this new macrophage differentiation system, an unlimited number of custom-designed macrophages with defined functional characteristics can be generated from any targeted ES cell. In combination with knockout or small interfering RNA knockdown technologies, this macrophage differentiation system provides a powerful tool for high throughput analysis of regulatory mechanisms controlling macrophage activation in health and disease.
View details for DOI 10.1189/jlb.0906590
View details for Web of Science ID 000244505800017
View details for PubMedID 17158607
Oxidative metabolism and PGC-1 beta attenuate macrophage-mediated inflammation
2006; 4 (1): 13-24
Complex interplay between T helper (Th) cells and macrophages contributes to the formation and progression of atherosclerotic plaques. While Th1 cytokines promote inflammatory activation of lesion macrophages, Th2 cytokines attenuate macrophage-mediated inflammation and enhance their repair functions. In spite of its biologic importance, the biochemical and molecular basis of how Th2 cytokines promote maturation of anti-inflammatory macrophages is not understood. We show here that in response to interleukin-4 (IL-4), signal transducer and activator of transcription 6 (STAT6) and PPARgamma-coactivator-1beta (PGC-1beta) induce macrophage programs for fatty acid oxidation and mitochondrial biogenesis. Transgenic expression of PGC-1beta primes macrophages for alternative activation and strongly inhibits proinflammatory cytokine production, whereas inhibition of oxidative metabolism or RNAi-mediated knockdown of PGC-1beta attenuates this immune response. These data elucidate a molecular pathway that directly links mitochondrial oxidative metabolism to the anti-inflammatory program of macrophage activation, suggesting a potential role for metabolic therapies in treating atherogenic inflammation.
View details for DOI 10.1016/j.cmet.2006.05.011
View details for Web of Science ID 000238821800006
View details for PubMedID 16814729


text:
----- Original Message -----
From: "Ran Lydell" <firstname.lastname@example.com>
Sent: Tuesday, July 09, 2002 9:20 AM
Subject: Re: First Look
> Just back from another great "First Look" If you didn't attend, you
> some fantastic new hostas that have not been seen in public before. We had
> great food and the hotel was memorable. An almost unbelievable array of
> plants were for sale in the vending area. many things that are not "on
> market" were for sale . Each attendee got a free new hosta . These were
> provided from Mark Zilis. The plant is 'Sugar Daddy' a white edged sport
> 'Big Daddy' I can't wait to see this one when it is older! There was
> raffle, ( lots of really great stuff in there) and some programs. The
> event allows everyone ( almost) plenty of time to talk. There were four
> "big" prizes again this year. I will let someone else tell about those.
> Carol & Bill were again responsible for the event and the wonderfull & fun
> results. In addtion many other folks were there, adding neat and very
> helpfull touches. One of those was Altera. All the grand prize ribbons
> one of her little paintings in the center . What a great treat for those
> us that were lucky enough to get one. I am already looking forward to
> this again. My personal thanks to everyone who worked so hard to make
> a first class event. and my sympathy to all those who missed out.
> Thanks
> Ran
To sign-off this list, send email to firstname.lastname@example.com with the
message text UNSUBSCRIBE HOSTA-OPEN


text:
Winnie Dress
ERIN erin fetherston
- Self: 63% cotton , 34% poly , 3% spandex
Lining: 98% poly , 2% spandex
- Fully lined
- Dry clean only
- Shirred skirt waist
- Jacquard fabric
- Back cut outs with bow accents
- Revolve Style No. EERI-WD60
- Manufacturer Style No. E40211274230
- Modeled in size 2.
- Shoulder seam to hem measures approx 34" in length
- Model Measurements: Height 5'9", Waist 24", Bust 32", Hips 34"
- Size Guide
- Need size help? Contact our Style Expert!
Girlish frocks for Hollywood sprites and anyone aspiring to become one, Erin Fetherston balances fairy tale with sensibility.
If an item you would like to purchase is out of stock, you can place a Special Order Inquiry. REVOLVE will then check the availability and obtain an approximate arrival date for the item, if available. Typically, it takes up to 3-4 weeks to verify this information.
Placing a Special Order Inquiry is absolutely free and there is no obligation to follow through with a purchase.
- You will be notified by email once we receive the item.
- You may cancel at any time without penalty
- Your card will ONLY be charged when your order is shipped.
- Availability is not guaranteed.


text:
Matching: license X
- Sort by:
- Date ▼
- Title
- Likes/Votes
- Comments
- Views
Rational License Availability Report
Saurabh.Tyagi Tags: rational availablity key center licenses account order id reports license rational-client-support 3,604 Views
License Availability Report: I am sure we all want to know, how our "IBM Rational product licenses are being used" it would be useful to track what licenses your company have purchased year by year and what is the available count in the...Modified on by AcdntlPoet
FAQ: Resolution of tsm error message "ANR2841W Server is NOT IN COMPLIANCE with license terms" Community Blog
After installed TSM server, the server keeps prompting an error message of server is not compliance as below: ***************************************************************************** ANR2841W Server is NOT IN COMPLIANCE with license terms...
from Blog: IBM Ecosystem Development Kuala LumpurModified on by zarinMY
Using temporary and permanent licenses for IBM products
NikhilBhat Tags: rational-client-support evaluation rational rational_perm.dat rational_server_perm.dat how-to license rational_temp.dat server rational_server_temp.dat temporary 3,557 Views
Of late I have come across instances, where license administrators are facing issues related to permanent and temporary licenses. I thought it will be a good idea to provide all the relevant information through this post. Let me start with a description...Modified on by AcdntlPoet
Coexistance of ILMT 9.x with IEM for SUA 9.x Community Blog
Silviu Gligore Tags: metric endpoint analysis software use tool ibm for manager license 3,626 Views
Hello World! :) I have had an issue the other days about using the new ILMT 9.x (IBM License Metric Tool) in parallel with IEM for SUA 9.x (IBM Endpoint Manager for Software Use Analysis). Even raised an APAR for it, APAR IV59595, raised to clarify this and...
from Blog: BigFixModified on by Silviu Gligore
50 DB2 Nuggets #21 : Tech Tip - Can you apply DB2 AESE license to ESE install or vice-versa?
MaryKKassey Tags: 50_db2_nuggets db2 ese license db2_techtip aese 4,798 Views
Hello! I would like to share with our readers the license issue that one of our customers ran into. Its straightforward and I'm sure you have seen this license problem before and know to resolve it but I would like to show the symptoms, what options we...
from Blog: Thoughts from SupportModified on by MaryKKassey
A New and Enhanced version of the RLKS Admin and Reporting tool has Released! Community Blog
Rational Common Licensing version 22.214.171.124 has released with valuable feature enhancements and defect fixes for the IBM Rational License Key Server Administration and Reporting Tool and the IBM Rational License Key Server Administration Agent. New features and...
from Blog: Rational Licensing Support CommunityModified on by jcbalin
Installing Rational Developer for i at latest level on multiple developer machines Community Blog
There are multiple methods for how to have Rational Developer for i installed and updated when considering a large number of developer machines. For an overview of the methods, please see http://www-01.ibm.com/support/docview.wss?uid=swg21668005 ,
from Blog: RDi – IBM Rational Developer for i HubModified on by EdmundReinhardt
IBM License Metric Tool (ILMT) services
Saurabh.Tyagi Tags: and audit reports rational the ibm ilmt tool d561hll. services unit number licensing value a in rational-client-support part metric sub-capacity processor pvu license environment 4,335 Views
One of the major initiatives many customers are pursuing is the widespread implementation of virtualization. Whether they are using virtualization purely to cut costs or whether it is part of their "green" agenda, this initiative will...Modified on by AcdntlPoet
HPC & Platform Cluster Manager 184.108.40.206 - Set up and manage license servers
YahengLiu Tags: pcm/hpc22.214.171.124 monitoring license 1,567 Views
In Platform HPC and Platform Cluster Manager 220.127.116.11 your cluster can monitor license servers. To monitor license servers, set up a Windows, UNIX, or Linux license server for your FlexNet products. In this example, the assumption is that a Linux license server...
from Blog: pdsh -a rebootModified on by helena_
Where are my licenses?
nsukhtan Tags: license-key-server reporting rational server-administration administration-reporting-... license 3,643 Views
Want to know how your IBM Rational product licenses are being used? Wouldn't it be useful to find out which users use which product licenses? How about knowing if any licenses were denied during peak hours? Or when the licenses that you...Modified on by AcdntlPoet
Top Rational Licensing technotes in November
AcdntlPoet Tags: top-support-content rational-licensing license licensing rational-client-support rational-license-key-serv... rational top-content 2,567 Views
Regardless of the Rational products you use, licensing topics are some of the most common questions Rational Support receives. These are the top 20 technotes for Rational Licensing that were reused during November to help solve your PMRs....Modified on by AcdntlPoet
Token licensing Open Mic session tomorrow
The IBM Rational Licensing Support Team in Bangalore is conducting an Open Mic session covering token licensing concepts. This session is helpful to those in that area looking for more information on the topic . ...Modified on by mquimby
The new Rational License Key Administration and Reporting tool has released! Community Blog
Download the the new Rational License Key Administration and Reporting tool for free today, and start reporting against your usage of IBM Rational Products. The reporting feature of the new Rational License Key Administration and Reporting tool generates...
from Blog: Rational Licensing Support Community
Open Mic sessions for Rational licensing coming soon!
The IBM Rational Licensing Support Team members are conducting Open Mic sessions that cover different aspects of Rational licensing and are helpful to those looking for more information on the topic . Here is an...Modified on by mquimby
Understanding the IBM Rational License Usage Order
Shijesh Velayudhan Tags: rational-common-licensing mapper lum settings rational usage administrator lkad license how-to rational-client-support key order licensing 4,703 Views
License Usage Order IBM Rational products requests licenses in a specific order. In an organization we may have combination of authorized user, floating, suite, point-product licenses in the environment, it is essential that we understand the...Modified on by AcdntlPoet
Mark your calendars! Three new OpenMic sessions next week!
AcdntlPoet Tags: rational-client-support open-mic license rational licensing openmic 3,842 Views
Greetings from IBM Rational Licensing Team! As a part of our proactive Client connects activities, we are conducting Open Mic sessions on licensing which covers different aspects of Rational licensing. We believe these sessions will be helpful for...
IBM Rational Doors Next Gen Licensing Overview
Ashok.Rao Tags: jazz rm team dwa requirements key license doors license-key-center client rational-client-support rrc gen web rational server rational-doors-ng access 3 Comments 14,676 Views
IBM Rational DOORS Next Gen is supported on Jazz platform helping you to collaborate with other Jazz platform tools like Rational Team Concert and Rational Quality Manager in your environment. IBM Rational DOORS Next Generation provides additional features...Modified on by AcdntlPoet
Rational License Key Center and its features overview
Saurabh.Tyagi Tags: rational-client-support accounts license rational order lkc preferences how-to keys 4,885 Views
License Key Center and its Features: Most IBM Rational software products require specific license keys in order to use them. The instructions discussed in this blog will guide you to the IBM Rational License Key Center (LKC), and the on-line service for...Modified on by AcdntlPoet
QRadar Risk Manager Tech Note: 'Risks' tab doesn't appear in QRadar console Community Blog
We released a new tech note today that addresses a common problem that occurs when customers begin deploying QRadar Risk Manager. http://www-01.ibm.com/support/docview.wss?uid=swg21635540 There is a common misconception that since QRadar Risk Manager ships on...
from Blog: QRadar on developerWorksModified on by MarkEhr
Jazz licensing: Exploring Rational Team Concert
Saurabh.Tyagi Tags: rational license rational-team-concert access client rtc developer licenses jazz rational-client-support 1 Comment 7,532 Views
Jazz LICENSING : Exploring Rational Team Concert Rational Team Concert has a simple set of defined roles to enable key solutions for your organization. With Rational Team Concert you simply acquire licenses to support the roles and solutions that you are...
Rational Test Workbench Licensing Overview
Ashok.Rao Tags: rational rpt rft rational-performance-test... rtw rtvs rational-test-workbench rit rpts license rational-test-virtualizat... rpt_pvu rational-client-support rational-integration-test... 7,196 Views
Rational Test Workbench Licensing Overview Now that Big Blue has worn a Green Hat and the products from Green Hat are now rolled into IBM's Rational division, let's take a deeper dive into the world of licensing concepts for a new origin product called...
IBM Rational Build Forge Licensing concepts
Saurabh.Tyagi Tags: edition standard licensing plus ibm rational-buildforge rational-client-support license enterprise rational 5,751 Views
As we are aware of that "IBM Rational Build Forge" is an adaptive process execution framework that automates, orchestrates, manages, and tracks all the processes between each hand off within the assembly line of software development, creating an automated...
Webcast poll results are in – Should you be worried? Community Blog
dwmigration2 Tags: software management asset license endpoint 574 Views
I wanted to share some interesting Webcast poll results with you on software audits and readiness. On Monday March 18 we conducted a Webcast on Software Asset Management with Frost & Sullivan and Deloitte. The focus was on the benefits of a comprehensive...
from Blog: IBM Redbooks Software Blog
Planning to open source your project? Here is what you should know Community Blog
punit_guron Tags: open project list documentation source mailing code license organization process 3,614 Views
I found a really informative article that explains in great depth about the points to consider while you plan to open source your project. It's a good read for those who are new to open source projects and want to learn about the process. The article starts...
from Blog: Real world open source
Systems Director on AIX - Is it no cost to run it on AIX?
At a recent POWER & AIX Workshop, I was challenged when I stated that you can download IBM Systems Director (ISD) and run it at no costs. Granted: If you want IBM Support for ISD functions then you do need to purchase the license and SWMA for every...
from Blog: AIXpert Blog


text:
US: +1 630 333 9501 UK: +44 208 123 1770 firstname.lastname@example.com
Global Robotics Market 2015-2019
Market outlook of global robotics market
Technavio's market research analysts estimate the global robotics market to witness a steady growth at a rate of around 26% during the forecast period. The growing adoption of industrial robots in developing countries is expected to drive this market during the forecast period. The market is seeing huge demands from the automotive, electrical and electronic and food and beverages industry in the developing countries. The US, Germany, and Japan are the major revenue contributors to the robotics market.
The economic boom in APAC has paved the way for industrialization and manufacturing. The increase in purchasing capacity has also led to a demand for high-quality consumer goods. Industrial robots are also replacing skilled laborers for various precision based roles such as welding and packaging.
Segmentation of the global robotics market by application type
- Industrial
- Service
The industrial segment is further classified into articulated, Cartesian, SCARA, and cylindrical while the service segment is subdivided into personal/domestic and professional.
Segmentation of the global robotics market by geography
- Americas
APAC is the fastest growing region globally, with countries like South Korea, China, Taiwan, and India emerging as the leaders in this region. Leading vendors such as ABB and Kuka are establishing the region as their base of operations. Moreover, governments' legislations and investments have enabled projects to enhance the infrastructure. These factors have made APAC the preferred manufacturing hub.
Key trends of the robotics market
In the recent years, there has been a significant increase in the number of robotic units sold in China. Government policies such as investment credits promoted the sales of local robots and led to an exponential growth of the local vendors. The country has also incorporated a large number of industrial robots into the industries due to growing labor cost and inflation. By the end of 2017, China is predicted to have the highest number of working robots in its manufacturing sector due to the steady adoption of automation into automotive and electronics industries.
This report provides some of the trends of the robotics market. It also offers an analysis of each trend and an estimation of the extent to which these trends are likely to impact the overall market growth.
Competitive landscape and key vendors
The robotics market is highly competitive with a large number of automation and robotics vendors. Some of these vendors specialize in providing industry-specific articulated robots to their end-users; creating a competitive edge over competitors. Most of the leading vendors in the robotics market are based out of the APAC region.
The top four vendors in the market are –
- Fanuc
- Yaskawa Electric
Other vendors in the market include Adept, Honda, Intuitive Surgical, iRobot, Kawasaki, and Epson Robotics.
Key questions answered in the report include
- What will the market size and the growth rate be in 2019?
- What are the key factors driving the global robotics market?
- What are the key market trends impacting the growth of the robotics market?
- What are the challenges to market growth?
- Who are the key vendors in this market space?
- What are the market opportunities and threats faced by the vendors in the robotics market?
- Trending factors influencing the market shares of APAC, EMEA, and the Americas?
- What are the key outcomes of the five forces analysis of the global robotics market?
Related reports


text:
UNIT 9.11 Natural Products as a Foundation for Drug Discovery
Published Online: 1 SEP 2009
DOI: 10.1002/0471141755.ph0911s46
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Pharmacology
Additional Information
How to Cite
Beutler, J. A. 2009. Natural Products as a Foundation for Drug Discovery. Current Protocols in Pharmacology. 46:9.11:9.11.1–9.11.21.
Publication History
- Published Online: 1 SEP 2009
- Published Print: SEP 2009
Options for accessing this content:
- If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team.
- Login via other institutional login options http://onlinelibrary.wiley.com/login-options.
- You can purchase online access to this Unit for a 24-hour period (price varies by title)
- If you already have a Wiley Online Library or Wiley InterScience user account: login above and proceed to purchase the article.
- New Users: Please register, then proceed to purchase the article.
Login via OpenAthens
Search for your institution's name below to login via Shibboleth.
Registered Users please login:
- Access your saved publications, articles and searches
- Manage your email alerts, orders and subscriptions
- Change your contact information, including your password
Please register to:
- Save publications, articles and searches
- Get email alerts
- Get all the benefits mentioned below!


text:
Indication/claims may vary by market
Managing the environmental impact of what we do is a high priority for Mölnlycke Health Care. We continuously work to prevent harm to the environment by adopting and implementing best practice at all of our sites worldwide. As a result of this, we have achieved a global ISO 14001 certification for the environmental management in our company.
Our environmental objectives
Our policy is to minimise the environmental impact of what we do, while continuing to deliver reliable products and services for our customers and patients. In order to achieve this, we:
Environmental management
Environmental management is a core part of our corporate management system. At a global level, we develop strategies, policies and objectives to ensure compliance and continuous improvement. At a local level, all of our sites are responsible for complying with environmental legislation, implementing the company policy and meeting our environmental objectives.
Sustainable material sourcing and product packaging...
Minimizing the use of potentially hazardous materials in manufacturing and products...
Reducing emissions, energy and water consumption...
You can contact our head office via the information provided below or by using the contact form. For contact information to local market offices, please refer to the company directory.
For product complaints or adverse events, please contact us using the Adverse event report form.
Mölnlycke Health Care ABBox 130 80SE-402 52 GothenburgSweden
Visiting address: Gamlestadsvägen 3C
Phone: +46 (0)31 722 30 00
Contact form
Postal address:Box 13080402 52 GöteborgSweden
Visiting address:Gamlestadsvägen 3C
© 2016 Mölnlycke Health Care
ATTENTION: Products shown may not be available in all countries. Indication claims may vary from country to country. For market specific product information, see the appropriate location section to this website.
Mölnlycke Health Care is a world-leading provider of single-use surgical and wound care products for customers, healthcare professionals and patients.
The content on this website is intended for healthcare professionals.


text:
Polarn O. Pyret
Polarn O. Pyret is a high-quality children's apparel retailer that began its children's clothing business in Europe, introducing its now-standard striped apparel collection in 1976. Today, the company offers t-shirts, sweatshirts, pants, jackets, and more from its 140 stores in eight countries, including five in the US with two in New York City, one in Edina, MN. and our newest in Chestnut Hill, MA.
- Phone:
- 617-651-8690
- Location:
- 55 Boylston Street Suite 5520
- Hours:
Mon–Sat 10AM-7PM
Sun 12PM-6PM
- Website:
- www.polarnopyretusa.com


text:
Likely to sell out
Granada: 2-Hour Guided Cathedral and Royal Chapel Visit
Duration: 2 hours
Explore Granada with a 2-hour guided visit to Granada Cathedral and the Royal Chapel. See the cathedral which took 181 years to build, and check out…
GranadaSee all 92 tickets & tours
Want to discover all there is to do in Royal Chapel of Granada? Click here for a full list.


text:
Photography Logo Design
This project was awarded to Djdesign for $55 USD.Get free quotes for a project like this
Project Budget$30 - $250 USD
Total Bids42
Project Description
Want a logo for a photography company. Needs to be custom, sleek, modern, minimalistic. Logo designer must be creative. Logo must flow well with website. The logo will be used to brand all digital and print media.
****Must show similar logo work to be chosen for project****.
I will disclose the photography website to the winner once the bid is accepted.
Winner will provide a minimum of 5 concepts for approval.
PSD and vector layers for submission.
I will retain all rights to the chosen logo.
Awarded to:
Skills Required
Looking to make some money?
- Set your budget and the timeframe
- Outline your proposal
- Get paid for your work
Hire Freelancers who also bid on this project
Looking for work?
Work on projects like this and make money from home!Sign Up Now
- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online


text:
Hi, list members,
I am relatively new to the hosta world and certainly know nothing
about botanical nomenclature, but I can read!
The introduction to the LIST OF REGISTERED CULTIVARS, on p.iv, says
"cultivar names follow the rules of the International Code for
Nomenclature of Cultivated Plants". On p. iii it says funding and
support for publication [including supplements] are provided by the
American Hosta Society. Registration is an INTERNATIONAL process,
so if a hybridizer from Japan or Germany, etc. is the one to register
a particular hosta, obviously they would name it in the language of
their own country. On p. v, the species selections/cultivars which
are registered are listed: e.g. H. nigrescens 'Elatior' (near the
bottom of the page. nigrescens is in italics. So despite my being a
newbie (please forgive me for saying this!), it appears that is the
correct way to list this hosta under recent discussion.
However, I also notice H. montana 'Praeflorens' ...and I believe
that has been changed to H. montana 'Macrophylla'? So am I opening a
new can of worms?
Maybe I need to practice my reading a little more!
Linda Kofstad
To sign-off this list, send email to firstname.lastname@example.com with the
message text UNSUBSCRIBE HOSTA-OPEN


text:
Want to renew your focus on Jesus during Lent? Sign up for our free Lent & Easter devotions.
2:3 his mighty men. Probably a reference to the Lord's army (v. 2.). The warriors and chariots of the attackers fulfill His purposes. The armies of Assyria herself had earlier been God's instruments to accomplish His purposes of judgment (Is. 10:5–7).
red . . . scarlet. These terms emphasize the awe-inspiring appearance of the approaching army, whether the color refers to their actual robes or to the blood stains on them.
spears are brandished. The Hebrew is difficult but conveys the general impression of ready eagerness for war.
Starting your free trial of Bible Gateway Plus is easy. You're already logged in with your Bible Gateway account. The next step is to enter your payment information. Your credit card won't be charged until the trial period is over. You can cancel anytime during the trial period.
Click the button below to continue.
You've already claimed your free trial of Bible Gateway Plus. To subscribe at our regular subscription rate of $3.99/month, click the button below.
It looks like you're already subscribed to Bible Gateway Plus! To manage your subscription, visit your Bible Gateway account settings.
Try Bible Gateway Plus, a brand-new service that lets you experience Bible Gateway free of banner ads! It also gives you instant access to over 40 Bible study and inspirational devotional books, including the NIV Study Bible. With Bible Gateway Plus, you can experience and understand God's Word in life-changing new ways, without the distraction of ads. Try it free for 30 days—you can cancel at any time. Following your 30-day free trial, Bible Gateway Plus is only $3.99/month.
Three easy steps to start your free trial subscription to Bible Gateway Plus.


text:
Coming up in the next {{countdown}} {{countdownlbl}}
Coming up next:
Skip to this video now
Now Playing:
Play Video
This transcript has been automatically generated and may not be 100% accurate.
Exclusive: Giffords' Husband Says She Feared She Would Be Shot
Mark Kelly unsure wife should stay in Congress; shares details on her recovery.
Heroes of Tucson: 'Something Had to Be Done'
Bill Badger helped subdue shooter, bullet grazed back of his head.
EXCLUSIVE: Gabrielle Giffords' Husband Capt. Mark Kelly Speaks to Diane Sawyer
Capt. Mark Kelly opens up to Diane Sawyer one week after the tragic shooting.
A 'Romantic' Date on Death Row
Part 2: Mark Kelly and Gabrielle Giffords had a date at a state penitentiary.
'She Squeezes Your Hand'
Part 3: Despite her serious brain injury, Giffords can follow simple commands.
Giffords' Stunning Prediction
Part 4: Kelly said Giffords worried someone would threaten her with a gun.
As Many Days 'As It Takes'
Part 5: NASA mission notwithstanding, Kelly says he'll stay by wife's bedside.
Now Playing: A 'Romantic' Date on Death Row
Now Playing: 'She Squeezes Your Hand'
Now Playing: Giffords' Stunning Prediction
Now Playing: As Many Days 'As It Takes'
Now Playing: Congresswoman and Astronaut
Now Playing: Hear O.J. Simpson speak for the first time under oath
Now Playing: O.J. Simpson: Inside the case of the defense 'dream team'
Now Playing: What life is like today for Nicole Brown Simpson, Ron Goldman's families
Now Playing: What O.J. Simpson said when asked about murder evidence
Now Playing: O.J. and Nicole Brown Simpson's relationship before tragic murder
Now Playing: The horrific day Leanna Taylor found out her son was dead: Part 1
Now Playing: Police start to suspect Justin Ross Harris after son's death: Part 2
Now Playing: Questions raised about Leanna Taylor's behavior after son's death: Part 3
Now Playing: Justin Ross Harris goes to trial for son's hot car death: Part 4
Now Playing: Why Leanna Taylor took the stand in ex-husband's defense
Now Playing: Leanna Taylor's mission to preserve son's legacy: Part 6
Now Playing: Larry Birkhead on Anna Nicole Smith, daughter Dannielynn today: Part 1
Now Playing: Anna Nicole Smith's legal battle for her late husband's money: Part 2
Now Playing: Larry Birkhead describes the first time he met Anna Nicole Smith
Now Playing: Anna Nicole Smith 'inconsolable' after son Daniel's death: Part 4
Now Playing: {{itm.title}}


text:
I've been wanting to hunt antelope for a few years prior to even becoming a hunter. I live in Colorado and am looking for a leftover or OTC doe tag for whyoming or possibly Colorado.
Im not looking for a trophy, just looking for my best chance of success. I have read good things about antelope Over the border. Any advice/knowledge is welcome.


text:
Furl or set your jib from your cockpit
Harken small boat furlers allow the sail to be set and furled from the cockpit, a critical convenience in many racing dinghies and trailerable sailboats. These systems, now sold as drum and swivel kits, are for roller furling, not for reefing. Constructed of Hardkote anodized Teflon-impregnated aluminum, they feature multiple stacked races of Delrin or Torlon ball bearings for smooth rotation under load. The jib's luff wire must be properly seized to the sail, since it carries most of the headstay load. You must supply the drum furling line in the diameters shown in the chart.


text:
User Agent:
Steps to reproduce:
1) Open two groups in Panorama. Each group with one tab.
2) Click on one tabitem
3) In the normal browser mode,
4) Close/restart Fx.
5) Close the last tab.
Actual results:
No tab item is selected.
Expected results:
A tab item is selected.
Related to
Bug 705621 - No tab item is selected after removing last tab in a group outside Panorama
Mozilla/5.0 (X11; Linux x86_64; rv:11.0a1) Gecko/20111204 Firefox/11.0a1
Confirmed using Stefan's STR.
Created attachment 579009 [details] [diff] [review]
Comment on attachment 579009 [details] [diff] [review]
Review of attachment 579009 [details] [diff] [review]:
Nice, thanks! Please add a test for this.
::: browser/base/content/browser-tabview.js
@@ +132,4 @@
> if (self._closedLastVisibleTabBeforeFrameInitialized) {
> self._closedLastVisibleTabBeforeFrameInitialized = false;
> + win.UI.showTabView(false);
> + win.UI.setActive(win.GroupItems.getActiveGroupItem());
Please move this to UI.init() and wrap it in 'if (!GroupItems.getActiveGroupItem()) { ... }' if that works.
Created attachment 579615 [details] [diff] [review]
I've looked into this and fixed the root cause. Also, added a test.
Comment on attachment 579615 [details] [diff] [review]
Review of attachment 579615 [details] [diff] [review]:
Nice, that fix is obviously much better. Thanks for digging into that!
Created attachment 579676 [details]
Patch for checkin
Pushed to try and waiting for results
Passed Try!
Sorry, didn't mean to mark it as fixed, yet.


text:
Finance and Accounting
Found 269 briefings
Finance and Accounting
This Road to Automation report, produced by Financial Director in association with Concur, considers the different tools and methods that finance teams use to manage and control employee spend and supplier invoicing, while assessing how much automation has had an impact on their finance function... view abstract
Company: Concur | Published: 23 February 2017 | Business Area: Finance and Accounting
This e-book provides insights into how using technology can help to keep the tax man happy. Discover the benefits of an expenses policy, what to check to ensure you are HMRC compliant and what VAT can be reclaimed on expenses.
Company: Concur | Published: 19 October 2016 | Business Area: Finance and Accounting
The first blog in the 'Marginal Gains' series introduces the marginal gains philosophy: a globally recognised business strategy that has transcended industries and powered some of the biggest British success stories of all time.
Company: IRIS | Published: 08 August 2016 | Business Area: Finance and Accounting
The modern finance organisation will play two key roles in the future that of a business partner and a business leader –which requires a higher level of proactive thinking and business skills than the partner stage. This Ovum paper looks at the key changes to hit finance thinking in 2016 and beyond... view abstract
Company: Oracle | Published: 26 July 2016 | Business Area: Finance and Accounting
We've examined some of the factors that you and your clients will need to consider if you want to hit the ground running as the new regulations come into force. By preparing for the new FRS now, you can avoid any negative impact on your day-to-day business later, and the right technology and know... view abstract
Company: IRIS | Published: 13 July 2016 | Business Area: Finance and Accounting
The accountancy world is evolving and don't we know it. You can't miss the articles, blogs and books about the latest trends. And you've probably seen the impact of these changes at first hand in your own practice. The latest technologies have transformed financial accounting into a commodity. All... view abstract
Company: Exact | Published: 12 July 2016 | Business Area: Finance and Accounting
Although we live in a digital age, many business processes, including supplier invoices, are still plagued by paper. This white paper addresses the flaws of the paper/email method and how it opens the business up to errors and risks. Learn how AP automation can relieve your business of such risks... view abstract
Company: Concur | Published: 29 June 2016 | Business Area: Accountancy
Transitioning to the new FRS could see your accounts preparation taking significantly longer; are you ready for the changes? Are you happy with them? This blog post highlights the danger of not preparing for new UK GAAP in advance and the FRC's decision to allow feedback on the new standards.
Company: IRIS | Published: 17 June 2016 | Business Area: Finance and Accounting
IFRS 9 is a new international standard set forth to address the weaknesses of IAS 39. This article provides an overview of the new standard and analyzes the major challenges financial institutions will face in ensuring compliance. While there is still uncertainty in terms of implementation approaches... view abstract
Company: Moody's Analytics | Published: 14 June 2016 | Business Area: Accountancy
In this article we have analyzed the use of macroeconomic scenarios as part of the forward-looking, probability-weighted IFRS 9 framework. Some of the key questions around the practical use of alternative scenarios and their probabilities have been answered, and a case study illustrates these concepts... view abstract
Company: Moody's Analytics | Published: 08 June 2016 | Business Area: Finance and Accounting
Find a finance and accountancy briefing
As a member you receive free access to our constantly updated library of white papers, analyst reports, case studies, web seminars and solution reports.


text: Attention Canadians: There is only one Vizio dealer in Canada that I know of (SIG Electronics in Toronto) and they've got a wicked deal on this TV right now. CAD$799 online [link] Apparently, they'll knock off some more $$$ if you buy it in the store. They also have a 37" Vizio LCD for $650. Read the RedFlagDeals thread for more.


text:
Women's Metallic Little Cities By Kangaroo Pendant Necklace In Sterling Silver
Petite and sweet. This polished pendant necklace from the Little Cities by Alex Woo collection boasts a dangling kangaroo charm set in sterling silver. Approximate length: 16 inches. Approximate drop: 3/4 inch. Photo may have been enlarged and/or enhanced. Color: metallic