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Pokémon Heroes: Latios & Latias 水の都の護神 ラティアスとラティオス Guardian Gods of the City of Water: Latias and Latios | ||||||||||||||||||
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Pokémon Heroes: Latios & Latias (Japanese: 劇場版ポケットモンスター 水の都の護神 ラティアスとラティオス Pocket Monsters the Movie - Guardian Gods of the City of Water: Latias and Latios; officially known as The Guardians of Altomare in Japan) is the fifth Pokémon movie and the final original series movie. It features Latios and Latias.
It was released in Japan on July 13, 2002. Distributed on limited release by Miramax Films, it opened in select American theaters on May 16, 2003. It was released on VHS and DVD in the United States on January 20, 2004. Echo Bridge Home Entertainment released the movie on Blu-ray (along with Destiny Deoxys on the same disc) on May 15, 2011.
Pokémon Heroes: Latios & Latias was shown with the Pikachu short Camp Pikachu.
Other posters and DVD covers
Spoiler warning: this article may contain major plot or ending details. |
Every year the city of Alto Mare holds a special Water Pokémon race through its canal streets—and this time around, Ash and Misty are top competitors! Even though he doesn't win, Ash still finds his own special place in the heart of a mysterious girl that he rescues from two roguish women. But this isn't any ordinary girl—it's actually the Legendary Latias in disguise!
Ash gets a special peek into the secret world of Latios and Latias, but this world is soon threatened by the two women from before—Annie and Oakley—who are after the two Legendary Pokémon, as well as a mysterious jewel called the Soul Dew. These elements combined will let them control a powerful machine that normally protects against danger. When the machine malfunctions, though, it not only puts Latios and Latias at serious risk, but the entire city of Alto Mare!
The main film's setting is in the water-themed city of Alto Mare, Johto. The city is watched over by two Legendary Pokémon, Latias and Latios. The story behind the two involves an evil Pokémon Trainer using a Kabutops and an Aerodactyl to terrorize the citizens, until the original Latios came to the city, using his powers to drown the evil Pokémon and turning the streets into canals. However, Latios died and his children were left orphans. His soul is within a special jewel, the Soul Dew.
The Soul Dew is sought by Annie and Oakley, two members of Team Rocket, and acts as a key for activating the Defense Mechanism of Alto Mare (DMA), a special device built in case of disaster. Meanwhile, Ash, Misty, and Brock are visiting Alto Mare on their travels. Ash and Misty participate in the Tour de Alto Mare, a water chariot race. Misty wins, after Ash takes a wrong turn due to an invisible Latias and Latios. Ross, the former champion of the race, gives the group a tour of the city in his gondola and tells them of the city's guardians. Annie and Oakley follow Latias, disguised as a girl named Bianca. Latias is saved by Ash and his Pikachu. Team Rocket are also in the city and decide to follow Annie and Oakley.
The trio later visit Lorenzo, the curator of a local museum, who explains the city's history and also introduces them to the Defense Mechanism of Alto Mare. Ash pursues Bianca, and then Latias in disguise, across the city, eventually discovering a hidden garden where Latias and Latios live. When Latios and Bianca threaten to force Ash out, Lorenzo arrives to clear up all the tension. Latias and Latios play with Ash and Pikachu, and Lorenzo later reveals the Soul Dew to Ash. However, Annie and Oakley's spy robot is watching this, and the duo soon raid the garden, stealing the Soul Dew, capturing Latios, and using both to control the DMA. Latias flees to Ash for help, so they race across the gridlocked city to the museum. Using the DMA, Oakley locks down the city and revives the dead Pokémon to capture Latias. After a long chase through the city, Ash and Latias cause the undead Aerodactyl to crash into a building, and are saved from the Kabutops by their friends' Pokémon. The heroes arrive at the museum to find the DMA going berserk and Latios seriously injured. After freeing Latios, the Soul Dew fades and turns black, causing the DMA to shut down. When Annie tries to get the Soul Dew, it disappears and the city's water becomes a giant tsunami. Latios and Latias use their psychic powers to stop the tsunami, but Latios sacrifices himself in the process. The water returns to the city, washing away Team Rocket in the process. The undead Pokémon return to the museum as Fossils, and Annie and Oakley become trapped inside the DMA.
Latios becomes a new Soul Dew and the new guardian of Alto Mare. Ash, Misty, and Brock start heading off to leave Alto Mare, but Bianca (or Latias disguised as Bianca) stops them before they leave and hands Ash a drawing of him and Pikachu. Saying nothing, she gives Ash a kiss on the cheek before running off, leaving the group wondering who it really was. As the group leave on a boat, they see two Latios and a Latias in the air. Annie and Oakley are sent to jail, where they examine the possessions of Lawrence III from The Power of One.
Major events
- Misty's Corsola is revealed to know Bubble Beam.
Pokémon debuts
- Pikachu (Ash's)
- Meowth (Team Rocket)
- Togepi (Misty's)
- Wobbuffet (Jessie's)
- Totodile (Ash's)
- Politoed (Misty's)
- Corsola (Misty's)
- Crobat (Brock's)
- Latios (anime)
- Latias (anime)
- Espeon (Annie's)
- Ariados (Oakley's)
- Vulpix (old lady's)
- Vaporeon (unknown Trainer's)
- Aerodactyl
- Kabutops
- Mantine
- Yanma (multiple)
- Butterfree (multiple)
- Chinchou (multiple)
- Murkrow (multiple)
- Oddish (multiple)
- Pidgey (multiple)
- Poliwag (multiple)
- Quagsire (multiple)
- Remoraid (multiple)
- Wooper (multiple)
Intro only
- Jigglypuff (anime)
- Caterpie
- Beedrill (multiple)
- Diglett (multiple)
- Geodude (multiple)
- Graveler
- Golem
- Gengar
- Lapras (multiple)
- Seaking (multiple)
- Rapidash (multiple)
- Rhyhorn (multiple)
- Seel (multiple)
- Dewgong (multiple)
- Spearow (multiple)
- Onix
- Tentacool
- Porygon
- Scyther
- Slowbro
- Starmie
- Voltorb
- Electrode
- Weezing
- Gyarados
- Victreebel
Tour de Alto Mare only
- Wailmer (Ross's)
- Wartortle (Competitor's)
- Golduck (Competitor's)
- Poliwrath (Competitor's)
- Tentacool (Competitor's)
- Starmie (Competitor's)
- Magikarp (Competitor's)
- Vaporeon (Competitor's)
- Chinchou (Competitor's)
- Lanturn (Competitor's)
- Azumarill (Competitor's)
- Quagsire (Competitor's)
- Qwilfish (Competitor's)
- Mantine (Competitor's)
- Seadra (Competitor's)
- Natu (multiple)
- Xatu
- Aipom
Cast | |||||
Ash | Veronica Taylor | Satoshi | Rica Matsumoto | サトシ | 松本梨香 |
Pikachu | Ikue Ohtani | Pikachu | Ikue Ohtani | ピカチュウ | 大谷育江 |
Misty | Rachael Lillis | Kasumi | Mayumi Iizuka | カスミ | 飯塚雅弓 |
Togepi | Satomi Kōrogi | Togepi | Satomi Kōrogi | トゲピー | こおろぎさとみ |
Brock | Eric Stuart | Takeshi | Yūji Ueda | タケシ | うえだ ゆうじ |
Jessie | Rachael Lillis | Musashi | Megumi Hayashibara | ムサシ | 林原めぐみ |
James | Eric Stuart | Kojirō | Shin'ichirō Miki | コジロウ | 三木眞一郎 |
Meowth | Maddie Blaustein | Nyarth | Inuko Inuyama | ニャース | 犬山イヌコ |
Bianca | Tara Jayne | Kanon | Fumiko Orikasa | カノン | 折笠富美子 |
Latias | Megumi Hayashibara | Latias | Megumi Hayashibara | ラティアス | 林原めぐみ |
Latios | Megumi Hayashibara | Latios | Masashi Ebara | ラティオス | 江原正士 |
Annie | Megan Hollingshead | Zanner | Uno Kanda | ザンナー | 神田うの |
Oakley | Lisa Ortiz | Lions | Yumiko Shaku | リオン | 釈由美子 |
Lorenzo | Wayne Grayson | Vongole | Yūzō Gucci | ボンゴレ | グッチ裕三 |
Ross | Michael Sinterniklaas | Rossi | Kōichi Yamadera | ロッシ | 山寺宏一 |
Narration | Ken Gates | Narration | Unshō Ishizuka | ナレーション | 石塚運昇 |
- This film uses the same intro music of Celebi: Voice of the Forest in the dub.
- Celebi: Voice of the Forest was released internationally after this film was released in Japan.
- Like the previous films, this one briefly displays Porygon at the beginning.
- This is the first movie not to feature a remixed version of the season's theme song during the opening credits; instead, an extended version of Believe in Me with no new lyrics is played.
- This is the first movie in Japan to use a different opening theme than the opening used at the time in the main anime; in this case, using the 2002 remix of Aim to Be a Pokémon Master rather than Ready Go!, which has been used in the anime for four months by the movie's release.
- Director Kunihiko Yuyama looked to Venice, Italy for inspiration when designing Alto Mare.
- This explains why the characters say ciao and arrivederci, as well as the names of Bianca and Lorenzo.
- This movie begins the tradition of featuring a next-generation Pokémon during the last movie in the current generation's series.
- This is the first movie in which Team Rocket has no contact with the main characters. It is also the first movie in which they are not the last characters to appear before the credits.
- The English dub of this movie mentions that an Aerodactyl and Kabutops drowned. Though it is possible that Aerodactyl (a Rock/Flying type) drowned, it is highly unlikely that Kabutops (a Rock/Water type) would have.
- The reanimated Aerodactyl and Kabutops most likely reference the fossils visible on display in the Pewter City Museum in the games released at this time (and possibly reference MissingNo. and its use of the fossils' sprites as two of its forms as well).
- Cyrillic characters appear on the right side of Oakley's computer screen while she is deciphering the code on the stone plates.
- In this movie, as in its predecessor Celebi: Voice of the Forest, Jessie, James, and Meowth land on a protruding object that only breaks when Wobbuffet comes out of its Poké Ball.
- Ash continues his habit of ramming obstacles, this time throwing himself into a barrier shielding Latios.
- In a throwback to The Power of One, Annie and Oakley read about Lawrence III in prison during the credits.
- This is the first movie to feature the permanent death of a Pokémon, in this case Latios.
- Misty refers to the Pokémon world as Earth during Latios's final sight sharing.
- Images of Mewtwo, Lugia, Entei, and Celebi are hidden in this movie, referencing Pokémon featured in the previous films.
- Pokémon Heroes was the last Pokémon movie to receive a theatrical release outside Japan until White—Victini and Zekrom, nearly nine years later.
- This is the fourth Pokémon movie to air on Toon Disney. The first three were Jirachi: Wish Maker, Destiny Deoxys, and Celebi: Voice of the Forest.
- This is one of the first two Pokémon movies to be available on Blu-ray, in a two-pack with Destiny Deoxys, in May 2011.
- Although the main anime switched its animation process to digital animation, this film, along with the entirety of the Advanced Generation series movies were still made with inked-and-painted cels. This changed with the release of The Rise of Darkrai in 2007, which was the first movie to be completely made with digital animation.
- During the opening scene, Brock narrates how Ash met Pikachu as the events of the first episode are shown with new animation, but fraught with inconsistencies and errors.
- Ash is pictured fully clothed when he receives Pikachu, but he was in his pajamas at that point in the episode. Also in this scene, Pikachu's Poké Ball surges with electricity as Ash holds it and Pikachu shakes his head after being released, neither of which occur in the episode.
- In the very next scene, Ash and Pikachu are running on foot from the flock of Spearow while a storm covers the sky, but the storm doesn't occur until after Ash takes Misty's bike in the episode.
- Ash's hat faces forward during this entire cinematic, even while Ash and Pikachu are lying on the ground after defeating the Spearow. In the episode, he turns it backward right before jumping into the river with Pikachu and it stays that way for the rest of the episode (except for the very end, when his hat erroneously flips back and forth between scenes).
- In one scene of Oakley typing on her computer, several keys on the keyboard disappear and reappear.
Dub edits
- Brock narrates the opening of the movie instead of the narrator.
- The entire backstory of the movie was changed in the dub. A segment of approximately two minutes, which told the story of the origin of the Soul Dew in Alto Mare's ancient history, was cut. This cut also removed the reference to the fact that many Latios and Latias visited and left Alto Mare frequently, causing some confusion to dub watchers when two Latios and a Latias appeared in the credits. This scene is very unique because the animation was done in a storybook-like fashion, something that was only done one other time, when it did appear in the dub.
- A replacement backstory was added in the form of a story about an evil Trainer who once attacked the people of the city with a Kabutops and Aerodactyl until Latios brought water to the town, drowning the evil Pokémon and turning the streets into canals.
- In the dub, the Soul Dew is the soul of Latias and Latios's father rather than the soul of one of their ancestors.
- The dub makes Annie and Oakley members of Team Rocket, but in the original, the two are working independently and never mention a relationship with Team Rocket or Giovanni.
- Masashi Ebara and Megumi Hayashibara provide the voices for Latios and Latias in the Japanese version, but Hayashibara provides each Pokémon's voice in the English dub. As a result, Latios's voice sounds like a less feminine Latias.
- An intense blue tint was applied to the American DVD release of the movie, making most of the colors much darker than in the original.
In other languages
Language | Title | |
Chinese | Cantonese | 水都的守護神 拉帝亞斯和拉帝歐斯 |
Mandarin | 水都的守護神 拉帝亞斯和拉帝歐斯 | |
Danish | Pokémon Heroes Filmen: Latios og Latias | |
Dutch | Pokémon 5: Helden | |
Finnish | Pokémon Heroes - Latias ja Latios | |
French | Canada | Pokémon 5: Les héros |
Europe | Les Héros Pokémon | |
German | Pokémon Heroes: Der Film | |
Greek | Πόκεμον: 5 Ήρωες Πόκεμον Ηρωες: Η Ταινία* | |
Hindi | Pokémon Movie - Soul Dew Ka Raaz: Latias and Latios * | |
Hungarian | Pokémon 5. - Új hős születik | |
Italian | Pokémon Heroes Pokémon: Latias e Latios* | |
Lithuanian | Pokemonai Didvyriai: Latijus ir Latija | |
Polish | Pokémon: Bohaterowie | |
Portuguese | Brazil | Heróis Pokémon |
Portugal | Heróis Pokémon | |
Russian | Покемон 5 Герои: Латиос и Латиас Покемон: Хранители водной столицы | |
Spanish | Latin America | Héroes Pokémon: Latios y Latias |
Spain | Héroes Pokémon | |
Swedish | Pokémon hjältar: Latias och Latios* Pokémon Heroes: Latios och Latias | |
Turkish | Pokémon 5. Film Kahramanlar: Latias ve Latios | |
Related articles
- Guardian Gods of the City of Water: Latias and Latios (manga)
- Guardian Gods of the City of Water: Latias and Latios (graphic novel)
External links
- Pokémon Heroes at IMDb
- Pokémon Heroes at Wikipedia
- Official website for Pokémon Heroes (English)
- Official website for Pokémon Heroes (Japanese)
This movie article is part of Project Anime, a Bulbapedia project that covers all aspects of the Pokémon anime. |


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Bowery Savings Bank
The Building was designated a New York City Landmark in March 1966.
Erected 1975 by New York Community Trust.
Location. 40° 43.119′ N, 73° 59.698′ W. Marker is in New York, New York, in New York County. Marker is at the intersection of Bowery Street and Grand Street on Bowery Street. Click for map. Marker is at or near this postal address: 130 Bowery Street, New York NY 10013, United States of America.
Other nearby markers. At least 8 other markers are within walking distance of this marker. Black Horse Inn (approx. 0.2 miles away); Kehila Kedosha Janina (approx. 0.2 miles away); 97 Orchard Street (approx. ¼ mile away); Edison Underground Central Station System (approx. 0.3 miles away); Engine Company 31 (approx. 0.3 miles away); Americans of Chinese Ancestry (approx. 0.4 miles away); Lin Ze Xu (approx. 0.4 miles away); Fluxhouse (approx. 0.4 miles away). Click for a list of all markers in New York.
Also see . . . Bowery Savings Bank - New York Architecture. Stanford White,
Categories. • Industry & Commerce • Notable Buildings •
Credits. This page originally submitted on , by Barry Swackhamer of San Jose, California. This page has been viewed 321 times since then and 17 times this year. Photos: 1, 2, 3. submitted on , by Barry Swackhamer of San Jose, California. • Bill Pfingsten was the editor who published this page. This page was last revised on June 16, 2016.

subset: commoncrawl


text: Nokia E71x on AT&T Delayed by Two Months or Morematt buchanan3/24/09 3:59pmFiled to: Nokia E71xe71xE71NokiaAt&tSymbiansymbian s6016EditPromoteShare to KinjaToggle Conversation toolsGo to permalinkSorry guys, that $200 Nokia E71x that was 'sposed to hit AT&T today isn't. Maybe for up to two months! AT&T wants a whole new firmware build before they'll release it. Oh, deja vu. [BGR] Reply16 repliesLeave a reply


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Description: Silver Creek Latigo Lace Remnants comes with assorted lengths and widths in neutral shades of Brown. Latigo Lace is a full-grain, 5 to 7 oz. leather lace, the strongest and most durable continuous leather lace available. Latigo lace is heavier than suede lace and is cut from smooth, top grain cowhide. 1 lb.
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Third time's a charm for Kelly Clarkson and Brandon Blackstock.
It's officially been three years since the pop star and her hubby tied the knot, and what better way to commemorate the special milestone than with a most timeless romantic gesture?! Clarkson celebrated the moment on Twitter Thursday morning, sharing a snapshot of a tree carved in the honor's couple.
"B + K= Love," the inscription read, which she captioned, "We got that young love.... Happy anniversary @BBlackstock We remembered this year"
With 2-year-old River Rose and newborn Remington Alexander to care for, as well as Clarkson's nonstop schedule, it's wonderful to see these two take some time for each other.
We got that young love.... ?? Happy anniversary @BBlackstock ?????? We remembered this year ?? pic.twitter.com/fkxpopIxyf
— Kelly Clarkson (@kelly_clarkson) October 20, 2016
Earlier this month, the "Piece by Piece" singer told Jenny McCarthy on her Sirius XM radio show their family of four was officially complete, revealing that not only did she get her tubes tied after Remi's birth in April, but she made her hubby get a vasectomy, too.
"I was literally pregnant with Remi, and I was like, 'You are getting fixed. This will never happen to me again,'" she revealed. "If something happens, it's a miracle of God. I literally told my OBGYN on the table while open, 'If I get pregnant again, I will find you!'"
And if Clarkson couldn't get more delightful, we fell in love with her unabashed honesty all over again when she took our E!Q in 42 just days ago.
Blazing through our questions about wake-up times, bed size, sex vs. naps, Prince William vs. Prince Harry and more, the talented perform and children's book author happily—and quite thoughtfully—shared all sorts of insight into what makes her tick.
So before heading off to wish Kelly and Brandon a happy anniversary, check out her hilarious tell-all here. Congratulations to the happy couple on a marriage well done!


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We need a laugh.
Categories: 1: Featured Arts and Culture Comedy Pop Culture
I was a devoted fan of The New Show during its brief run. The sketch with John Candy running a food repair show was comedy gold.
[3] Candy just sells that concept beautifully. What a terrific sketch performance.
[4] Don't get yer meaning.
It's interesting watching the stuff Lorne Michaels did during his exile from SNL - this show, with Dave Thomas and Buck Henry as the star regulars and then lots of familiar faces from SCTV, SNL & elsewhere as guests, Steve Martin's "Best Show Ever" prime time special, which also had a distinct 70s SNL feel to it...


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Data Profits's iKIS™ SaaS demand forecasting, inventory replenishment, and supply chain solution provides enterprise level performance without the enterprise price. Combining state-of-the-art forecasting algorithms with disruptive technology and a user-friendly interface allows you and your vendor partners to adapt to market changes before your competition. Our solutions for demand forecasting, inventory replenishment, inventory optimization, omni-channel, and lead time forecasting are provided on a web-based SaaS platform allowing for lightning fast installs, frequent hassle-free updates, easy vendor collaboration, and nearly immediate ROI.
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In Newark, drop a dime on a gun owner, get $1,000.
Notice Mayor Booker only waves around $500. Where's the other $500?
Bright idea number 1: Report a friend who has a gun permit. Split the $1,000. Or don't they issue gun permits in Jersey anymore?
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The Entree is an in depth documentary/ extended interview with Australian born UCI Downhill World Series Champion Sam Hill based on his 2009 racing season. With just three rounds to go and a winless and turbulent season already behind him The Entree begins in Mont Saint Anne, Canada round #6 where things begin to change and the race to the finish begins. Follow Sam and his team mate Brendan Fairclough across the globe on an insightful journey from Canada, back to his homeland Australia then to Schladming, Austria for the ride of the season on his quest for World Series Gold and the celebrations that followed. Music courtesy Wolfgang Gartner at Kindergarten Records.
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Closing out Summer on its last day with this week's installment of Wednesday's Whatevers. Where I live, Fall takes its time in coming and the hot weather will often linger into October. No matter, I will soon be doing my twice-a-year cycling of my closet, bringing down and out the boots and sweaters and packing away my sandals and bathing suits. A full disclosure on my closet is in the works but for now, I have a collection of links on ideas for being stylish, having fun, and celebrating the moment in an "it's all Fine, Whatever tickles the fancy" manner ready to share with you. In following with the format of the categories on the blog (see them here), for your reading pleasure I now present;
Whatever Hooks The Look
The theme for the Fine-Whatever link up for this coming Saturday (which is the fourth one of the month) is In Real Life. When I started this blog, I wanted it to cover more than fashion so with this theme I try to emphasize the occasion more than what I wore. These posts are a few that do this.
Whatever Hustles The Muscle
Who else is excited to have Dancing With The Stars as an alternative to Monday Night Football? Since dance is such a fun way to get in some aerobic exercise, I have a selection of dance related links.
Whatever Props The Shop
Last week I happened to have had dinner at a restaurant that was next door to a Marshalls. I haven't shopped at a Marshalls in years but I do frequent a TJ Maxx that is near the nail shop where I get my pedis. The Husband and I wandered into Marshalls for a quick look around and of course, I couldn't leave without buying a sleeveless sweater (on my wish list), a long sleeve cold-should top (not sure if cold-shoulder makes sense in cooler weather), and a pair of oxfords that feel like I'm walking on a cloud. The links below are to TJ Maxx but the items and prices are the same.
Whatever Skates The Plate
These are a few of the yummy recipes I came across this past week and are now on my "must try" list.
Whatever Spins The Grin
Sometimes I have a lot of options for this category and other times like this week, not so much. After poking around a bit, I found these amusements to share.
Those are the whatevers for this week, which one was your favorite?
Let's connect! Subscribe, tweet, follow, friend, love, pin, stumble … all options are available and waiting for you. I look forward to hearing from you.


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YouTube- Facebook project: upload, SEO
- Tila Suljettu
- Budjetti $10 - $1500 USD
- Tarjouksia yhteensä 3
Projektin kuvaus
YouTube- Facebook project: upload, SEO
This is an ongoing project which will repeat every year. My father is a South Asian musician based in North America who has grown quite popular on YouTube for the past 10 years. He has 500 subscribers and one million video views. He sings in the following music genres: ghazal, sufi, religious, bollywood, Pakistani songs, traditional folk, Indian songs, Punjabi, Hindi, and Urdu.
Your core responsibility as a person or agency is to create a brand new and much more advanced YouTube channel for my dad. We need you to re-upload the videos my dad has promoted for the past 9 and a half years on his new YouTube channel. Please note that you will re-upload his videos as well as new videos on his new YouTube Channel. You must edit each video by placing an advertisement of his new Facebook page before re-uploading all the videos on YouTube.
My dad's desire is to use new SEO techniques to ensure he receives maximum promotion on YouTube which will help him get noticed by the world. We both believe that technology on YouTube has improved since his channel's inception in 2007 and we need your help to increase his promotion. Bear in mind that my dad did not receive any form of promotion on YouTube from 2007 to 2017; all we did in promoting his music was the basic uploading videos and creating tags, but he still received one million views from this time period - we think it's an amazing achievement in of itself. With this in mind, a very important goal of your job is to help my dad reach a larger target market in a more efficient manner.
Job requirements
*YouTube and Facebook work hand in hand. You will promote my dad's music material on a new YouTube channel and Facebook page created by you.
*A fundamental requirement of this job is that you speak Hindi, Punjabi, and Urdu fluently; you must also be interested in Indian music, and you must have prior knowledge about promoting musicians via YouTube.
*Please note I am open to hiring people who don't speak Hindi, Punjabi, and Urdu, but this candidate must have a proven track record at promoting music artists on YouTube. I have to see your YouTube success stories.
*You must transfer YouTube content to Facebook for promotion.
*You will label all of my dad's songs (music videos) on YouTube by carefully following the instructions we send to you via email;
*You will add channel art to his new YouTube page;
*You will apply SEO techniques to each video you upload on his new channel: each video must contain several tags and keywords which will lead to highest potential results. The idea you should have in mind is to use effective tags in an effort to promote my dad's music and reach his target market all over the world;
*You are required to upload his videos in the highest quality;
*Above all, you must create ways to attract organic traffic for his new YouTube videos, that is real people who are interested in his music and want to see him perform in person.
I am willing to listen to your offer in our conversations on Freelancer. If you have a good track record at promoting musicians on YouTube, please discuss your quote with me.
*The exciting part of this project is that you can earn additional milestones which will celebrate your job success at the end of the project.
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I do not have a budget in mind so please be realistic with your offer. I can hire you and make a deposit as soon as possible.
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Former 37-year-old businessman Angelo Mastropetro decided to go back to the origins of nature after doctors diagnosed him with multiple sclerosis. For eight months he turned the 800-year-old cave into the dream house.
After investing $ 150 000 he turned the cave into luxury accommodation with a heated floor, summing up sewage and even wireless internet. Living close to nature, Mastropetro hopes to get rid of his illness.


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Nursing Home Providers in Ivydale WV
HealthCare4PPL is your most comprehensive directory of nursing home providers in Ivydale WV. HealthCare4PPL provides detailed information including facility overview, service rating and user survey details of 1 Medicare-certified and other kinds of nursing home providers.
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Clay Health Care Center
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The molybdenum nitrogenase is responsible for most biological nitrogen fixation, a prokaryotic metabolic process that determines the global biogeochemical cycles of nitrogen and carbon. Here we describe the trafficking of molybdenum for nitrogen fixation in the model diazotrophic bacterium Azotobacter vinelandii. The genes and proteins involved in molybdenum uptake, homeostasis, storage, regulation, and nitrogenase cofactor biosynthesis are reviewed. Molybdenum biochemistry in A. vinelandii reveals unexpected mechanisms and a new role for iron-sulfur clusters in the sequestration and delivery of molybdenum.
The scarcity of molybdenum in the Earth's crust belies its importance for the metabolism of living organisms and for the global biogeochemical cycles of major elements such as nitrogen, sulfur and carbon (1, 2). In contrast, many elements that are present in considerably larger amounts have not apparent biological function (e. g. Al, Ti or Zr) (3). Despite its low abundance - Mo ranks 53rd in the Earth's crust - molybdenum is more available to biological processes than many other metals, which are found in chemical forms difficult to assimilate. Molybdate is the predominant source of MoVI at neutral and basic pH. Other common sources of molybdenum are the highly insoluble molybdenite (MoS2) and wulfenite (PbMo4) ores (4). In marine environments, molybdenum is present at around 110 nM, being the most abundant transition metal in the sea (1). In terrestrial environments, molybdenum distribution is irregular, usually lower than in the marine systems, and estimated to be 50 nM on average. The importance of molybdenum in soil ecosystems has recently been highlighted by a study showing that molybdenum scarcity severely limits biological nitrogen fixation in tropical forests (5). Limitation of nitrogen fixation by molybdenum might be common in highly weathered acidic soils, which would hinder the ability of some forests to balance carbon and nitrogen (6).
Comparative genomic studies reveal that molybdenum metabolism is widespread in nature (7). Molybdenum is utilized - to different extents - by most organisms belonging to the three domains of life: archea, bacteria, and eukaryotes. Phylogenetic analyses reveal that most prokaryotes (archea and bacteria) and higher eukaryotes utilize molybdenum, whereas many unicellular eukaryotes, including parasites and some yeast, have lost their ability to use this metal.
In addition to being the only second row transition metal essential for life, molybdenum provides an extremely versatile building tool for the coordination chemist. The element has a [Kr] 4d5 6s1 electron configuration, and its chemical properties thus center on its half-filled 4d shell. Molybdenum is able to adopt oxidation states from (-II) to (+VI) together with ligand coordination numbers ranging from 4 to 8 and a variety of coordination geometries. It forms compounds with most inorganic and organic ligands, and a wide range of metal and mixed-metal clusters have been synthesized. The chemistry of the higher molybdenum oxidation states (MoVI, MoV, MoIV, MoIII) is dominated by oxo species, such as molybdates and polymolybdates, and terminal oxygen containing species like MoVIO2, MoVO, MoIVO and MoIVO2 comprise the central cations in a range of complexes (8). Molybdenum also has a rich and diverse sulfur chemistry, which comprises ligand-based redox behavior, internal electron-transfer processes and 'intermediate' redox states (9), all largely a consequence of the small energy gap between the sulfur 3p and the Mo 4d orbitals (10). The importance of molybdosulfur complexes in biological and industrial catalysis has led to the study of an array of monomeric (11), dimeric (12) and cluster complexes (13, 14).
Biologically active molybdenum is found in the cofactors of molybdoenzymes. Much of our understanding of molybdenum sites in enzymes is derived from combining the results of EPR1 (15-17) and ENDOR (18, 19) spectroscopies on paramagnetic enzyme intermediates, with structural information from extended X-ray absorption fine structure (EXAFS) spectroscopy (16, 20-24) and X-ray crystallography (25-28). In addition, the chemical behavior of related inorganic compounds has been used to calibrate the spectroscopies and to suggest or confirm structural and mechanistic possibilities for the enzymes (11, 14). These studies have shown that all molybdenum cofactors, with the exception of the FeMo-co of nitrogenase, are based on a unique tricyclic pterin (molybdopterin) and are generically termed as Mo-co (16, 24, 29). Mo-co containing enzymes catalyze a range of oxidation/reduction reactions in carbon, sulfur and nitrogen metabolism, such as the oxidation of hypoxanthine to xanthine, the reduction of nitrate to nitrite and the oxidation of formate to carbon dioxide (16, 24).
Fixed nitrogen is an essential component of amino acids, proteins, and nucleic acids in all organisms. It is also present in other essential molecules that are abundant in the biosphere, such as chlorophyll and heme groups. Although nitrogen gas (N2) constitutes 78% by volume of Earth's atmosphere, it is unusable by most organisms, which can only assimilate fixed nitrogen molecules. The low reactivity of N2 limits its conversion into fixed nitrogen molecules. Nevertheless, a special group of prokaryotic organisms has developed the ability to fix N2 at moderate temperature and pressure conditions in a process known as biological nitrogen fixation. Nitrogen-fixing organisms (diazotrophs) reduce N2 into NH4+ that is subsequently assimilated by themselves and by other organisms, such as plants, fungi, and animals. Thus, the global balance of nitrogen on Earth's biosphere relies on the capacity of diazotrophic organisms to serve as primary input of fixed nitrogen into the ecosystems (30).
The enzyme responsible for all biological nitrogen fixation activity is termed nitrogenase. The catalytic site of nitrogenase contains a complex metallocluster where N2 binding and reduction into NH4+ takes place. There are four classes of nitrogenase enzymes characterized so far. Three of them are homologous enzymes with similar - not identical - protein subunit composition and metal cofactor structure (31, 32); these are the Mo-nitrogenase, V-nitrogenase, and Fe-only nitrogenase. The Mo-nitrogenase, which contains the iron-molybdenum cofactor or FeMo-co, is the most commonly distributed nitrogenase; it is also the most efficient in the conversion of N2 into NH4+. Although most diazotrophs only have the Mo-nitrogenase, some of them also synthesize alternative V-nitrogenase and/or Fe-only nitrogenase enzymes, which contain the FeMo-co-like FeV-co or FeFe-co metalloclusters at their active sites, respectively. There are no reported diazotrophs lacking a Mo-nitrogenase and carrying uniquely an alternative nitrogenase. Regulation of nitrogenase expression in bacteria carrying alternative nitrogenases is dependent on the availability of molybdenum, vanadium or iron in the medium (33). These three nitrogenases consist of two component proteins that, in the case of the Mo-nitrogenase, are denoted as NifDK2 and NifH. The NifDK component accommodates the active site cofactor whereas the NifH component serves as specific electron donor to NifDK.
The FeMo-co active site of the Mo-nitrogenase is a complex Mo-Fe-S metallocluster comprising an inorganic Fe6-S9 core that coordinates a central light atom X (C, N or O) and is capped by external Fe and Mo atoms. A molecule of R-homocitrate coordinates the molybdenum atom through its C-2 carboxyl and hydroxyl groups to complete the cofactor (Figure 1) (27, 28, 34, 35). The role of the molybdenum sub-site in FeMo-co is unclear (31, 36). For some time, molybdenum was considered a likely locus for substrate binding and catalysis as the Mo-nitrogenase is substantially more active in N2 reduction than its V or Fe analogs, and the R-homocitrate ligand is essential for catalysis (31, 32). In addition, molybdenum compounds can readily coordinate N2 and its reduced forms, and this chemistry has been systemized into a putative mechanism termed the "Chatt cycle" (37). However, recent combined spectroscopic and substrate/inhibitor binding studies strongly indicate that the [Fe6-S9-X] core is the initial substrate binding site (31, 32, 36) whereas current mechanistic models do not include a direct function for the molybdenum sub-site (31).
The fourth type of nitrogenase is a Mo-nitrogenase phylogenetically unrelated to the other three classes that has only been found in the bacterium Streptomyces thermoautotrophicus. This Mo-nitrogenase exhibits completely different biochemical features that consist of different protein composition, insensitivity to O2, low Mg·ATP requirement, and a Mo-co type of cofactor at the active site (Mo-molybdopterin cytosine dinucleotide or Mo-MCD) (38).
The bacterium Azotobacter vinelandii has traditionally been one of the preferred models to study molybdenum metabolism in prokaryots because it carries several Mo-co-containing enzymes and a Mo-nitrogenase. The genetic components involved in molybdenum capture, trafficking, storage, metabolism, and regulation that are encoded in this organism have been studied for decades (Figure 2). Although studies in other diazotrophs such as Klebsiella pneumoniae and Rhodobacter capsulatus have provided significant insights into the metabolism of molybdenum, A. vinelandii will be used as the reference organism for discussion in this review. The recent release of the A. vinelandii genome sequence (39) has revealed the presence of new genes related to molybdenum, for example those encoding an anaerobic Mo-co-dependent formate dehydrogenase. This review will focus on those proteins and processes related - albeit not necessarily in an exclusive way - to molybdenum-dependent nitrogen fixation.
The average molybdenum concentration in soils (ca. 15 μmol/kg or 1-2 ppm) is the lowest among all transition metals having a biological role (40). Molybdate (MoO42-, MoVI) is the main source of molybdenum because it is soluble at neutral and basic pH. Other forms of molybdenum described to efficiently serve as assimilable molybdenum sources are MoO3, Mo-cysteine dimer, Mo-glutathione dimer, MoCl6, MoCl4 dipyridine, and MoS2 (41-43). In contrast to iron, which is poorly available in oxic soils due to precipitation of iron oxides and iron hydroxides, the oxoanionic molybdate is highly soluble. Its negative electric charge prevents adsorption onto particle surfaces at neutral and basic pH resulting in the formation of weak complexes with most organic ligands with the exception of catechols (44). The catechol groups of organic matter are able to bind molybdate over a wide pH range retaining molybdenum in the top layer of the soil (45).
Many bacteria are known to produce and secrete siderophores. Siderophores are small, high-affinity FeIII-chelating compounds secreted to the extracellular medium by organisms subjected to low iron availability (46). Besides chelating FeIII, siderophores are able to chelate molybdate, vanadate, and tungstate. The genome of A. vinelandii encodes the genes for the production of five different siderophores: the mono-chatecols aminochelin and 2, 3-dihydroxybenzoic acid (DHBA), the bis-chatecol azotochelin, the tris-chatecol protochelin, and the pyoverdin-like azotobactin (47). Genes involved in the production and excretion of catechol siderophores, including csbC (Avin21220) (48), and csbX (Avin21230) (49) had been previously reported. The siderophore DHBA is the one most abundantly excreted by A. vinelandii, but has low affinity for metals and its participation in sequestering molybdenum is limited (50). Protochelin, also abundantly excreted, dominates the speciation of iron, molybdate, vanadate and tungstate present in the medium, whereas aminochelin and azotobactin become dominantly excreted at extremely low iron concentrations (48, 51). Catechol siderophores play a crucial role in trace metal nutrition by binding oxoanions of molybdenum, vanadium or tungsten present either in solution, or attached to naturally occurring ligands, or bound to organic matter (47, 52). In addition, catechol siderophores have been proposed to be involved in detoxification of those metals that are deleterious to cell metabolism (53).
Bacteria scavenge molybdate from the environment by using high affinity ABC-type transport systems (54-58). These specialized Mo-uptake systems are required by bacteria to efficiently discriminate between molybdate and tungstate (53). Average soil tungstate concentrations are around 1 ppm, in the range of molybdate concentrations. Tungstate has similar structural properties to molybdate, but its uptake and incorporation into active-site cofactors yield inactive W-nitrogenase (59) and W-nitrate reductase (60) enzymes.
In addition to the high-affinity transporter specific for molybdate, the sulfate transporter has been suggested to serve as low affinity molybdate transport system, although this role has not been extensively characterized (33).
The high affinity ABC transporter is encoded by the modA, modB and modC genes present in the mod operon. The modA gene encodes a high-affinity molybdate-binding protein located in the periplasm; molybdate reaches the periplasm, presumably through porins, and then binds to ModA. The modB gene encodes an integral cytoplasmic membrane protein that provides the transport channel. The modC gene encodes the so-called conserved component, a membrane associated protein that binds and hydrolyzes Mg·ATP to provide energy for the active transport of molybdate. Unlike most microorganisms, A. vinelandii contains three copies of the modABC operon, revealing the importance and complexity of molybdenum metabolism in this microorganism. Even the closely related nitrogen-fixing strain Pseudomonas stutzeri contains a single copy of the modABC operon (61). The products of the A. vinelandii modA1B1C1 operon (Avin50670 to Avin50650) have been shown to be required for growth under conditions of molybdate limitation (58). The A. vinelandii genome sequence showed that the modA1B1C1 operon is located near the so-called minor nif cluster, which contains the nifB and nifQ genes among others. The second known mod operon, modA2B2C2 (Avin01300 to Avin01280), is located near the major nif operon. The genome sequence also revealed the existence of a third mod copy, modA3B3aB3bC3 (Avin50730 to Avin50700) located next to the modA1B1C1 operon (39).
In addition to the use of soluble molybdate ions, A. vinelandii cells are able to use up molybdate-siderophore complexes as molybdenum source (44). The mechanism of molybdate-siderophore utilization is not clear. Yet, on the basis of the known mechanism of iron transport systems, it has been suggested that siderophores transfer molybdate to the ModA protein located in the periplasm (47). Similarly, A. vinelandii cells are able to take up vanadate-siderophore complexes. On the other hand, the tungstate-siderophore complexes, which are deleterious to bacterial growth, are either poorly taken up (53) or not taken up at all (47).
The A. vinelandii ModG protein has been proposed to be responsible for the homeostasis of molybdenum in the cytoplasm (58). The modG mutants exhibit pleiotropic effects in nitrate reductase and nitrogenase activities that suggest a role for ModG in balancing molybdenum availability for the biosyntheses of Mo-co and FeMo-co (58). The A. vinelandii ModG protein is encoded by the Avin50690 gene, which is clustered with and divergently transcribed from modEA1B1C1. Each ModG monomer consists of a tandem repeat of two 65-amino-acid-long Mop domains, a structural fold that specifically binds molybdate and discriminates against other oxoanions. In its native conformation, ModG is a trimer that binds up to eight molecules of molybdate (62).
Mop domains can be found as stand-alone structures or in combination with other types of domains as part of larger proteins, such as in ModA and ModE (see below). The Mop domains are widespread in bacteria and have been thoroughly studied in A. vinelandii, Clostridium pasteurianum, Escherichia coli and R. capsulatus (63, 64).
The capacity of A. vinelandii to scavenge molybdate from the medium is remarkable. This bacterium can accumulate 25 times more molybdenum than it requires for maximum nitrogenase activity (42). The molybdenum storage protein (MoSto) is responsible for these high levels of molybdenum accumulation (65) and, to our knowledge, it has only been described in A. vinelandii.
The MoSto protein is an α3β3 hexamer of the mosA (Avin43200) and mosB (Avin43210) gene products that can store up to 100 molybdenum atoms per hexamer (66). MoSto can also incorporate tungsten. Metal storage within MoSto occurs in the form of a variety of compact polynuclear oxoanions (67). The incorporation of molybdate into MoSto is a nucleotide-dependent process (68). The release of molybdate from MoSto is, however, ATP-independent and appears to be pH-dependent and to occur stepwise, suggesting the involvement of several amino acid groups in the release mechanism (66, 69). No additional proteins seem to be required to load or unload molybdate from MoSto. Although MoSto expression is not controlled by nif regulatory factors (65), the level of molybdenum incorporation into MoSto increases in nitrogen fixing cells, consistent with a proposed role as a molybdenum reservoir destined to FeMo-co synthesis. Nitrogen fixation is a process requiring considerable amounts of molybdenum given that NifDK may represent up to 10% of the total protein content during diazotrophic growth.
The function and structure of the molybdate-responsive transcriptional regulator ModE has been well characterized in E. coli. ModE binds molybdate and represses the expression of modABC genes under molybdenum-replete growth conditions (70-72). In addition, ModE has been shown to coordinate expression of a number of molybdenum-dependent genes by repressing or activating their expression. The molybdate transporter genes, Mo-co biosynthesis genes (73), and several molybdoenzyme structural genes, such us the periplasmic and respiratory nitrate reductases (74, 75) or the dimethylsulfoxide reductase (76), are known to be regulated by ModE.
Each subunit of a ModE dimer consists of two Mop domains and a helix-turn-helix (HTH) DNA-binding domain (77, 78). Efficient DNA binding requires protein dimerization and molybdate binding; both functions are mediated by the Mop domains of ModE (78). Binding of molybdate to ModE drives extensive conformational changes in both the Mop domain and the DNA-binding domains that largely increase its affinity for specific operator sequences (72, 77, 79, 80).
ModE has been identified in a number of bacteria, including A. vinelandii (54). Moreover, ModE binding sequences have been found widespread in bacteria and archea (81). The A. vinelandii ModE is encoded by the Avin50680 gene located within the modEA1B1C1 operon. The modE mutant exhibits normal diazotrophic growth rates. However, A. vinelandii strains carrying mutations in both modE and modG genes are severely impaired in diazotrophic growth (58), indicating their involvement in nitrogenase biogenesis or regulation. The A. vinelandii genome carries an additional modE copy (Avin33430) adjacent to a V-nitrogenase transcriptional regulator vnfA2 (Avin33440). The role of this ModE homolog has not yet been characterized. The presence of putative ModE-binding sites upstream the vnfA2 and anfA genes, encoding regulatory proteins for the V-nitrogenase and the Fe-only-nitrogenase respectively, suggests that ModE coordinates the expression of all three nitrogenases in A. vinelandii in response to molybdenum availability (33).
The biosynthesis of the complex FeMo-cofactor involves the activities of a large battery of nif and non-nif gene products, including proteins that exhibit catalytic activity, proteins that act as molecular scaffolds, and proteins which role is to carry and protect FeMo-co intermediates between the sequential assembly sites (82). The genes encoding proteins involved in FeMo-co biosynthesis in A. vinelandii are located within two chromosomal regions equidistant from the origin of replication denoted as the major nif region (Avin01360 to Avin01710) and the minor nif region (Avin50990 to Avin51060) (39, 83-85).
The model for the FeMo-co biosynthetic pathway depicted in Figure 3 illustrates the convergence of the FeMo-co building blocks towards a central catalytic node consisting of NifEN and NifH. The NifU/NifS/NifB/NifX branch of the pathway provides NifB-co, a FeMo-co biosynthetic intermediate proposed to comprise the [Fe6-S9-X] core of the cofactor (86). At this stage, molybdenum has not yet been incorporated into the precursor (87). The specific role of the cysteine desulfurase NifS and the scaffold protein NifU is to provide [Fe2-S2] and/or [Fe4-S4] clusters that would serve as metabolic substrates for NifB to synthesize NifB-co (88). The synthesis of NifB-co by NifB is a redox and S-adenosyl methionine-dependent process that involves radical chemistry (89). It has been proposed that very low-potential radical chemistry carried out by NifB is responsible for the incorporation of the X atom into NifB-co (89). Although not essential, the carrier protein NifX has been shown to optimize the transfer of NifB-co from NifB to NifEN (90). NifEN transforms NifB-co into the VK-cluster, which is proposed to be the next intermediate in the biosynthetic pathway (90). Similar to NifB-co, the VK-cluster does not contain molybdenum or homocitrate. However, these two intermediates are electronically and structurally different: while NifB-co is EPR silent (91), the VK-cluster exhibits EPR signals in the dithionite-reduced and the thionine-oxidized states (90). EXAFS and NRVS analyses indicate that NifB-co is an [Fe6-S9-X] cluster (86) whereas VK-cluster structures containing this core plus one or two additional terminal Fe atoms are favored (92).
In the second branch, NifQ specifically donates molybdenum to the NifEN/NifH proteins (93). The molybdenum of NifQ is present in an [Fe-S] cluster environment (see below). It is currently unknown whether NifQ transfers its entire [MoFe3S4] cluster, a portion of the cluster or simply the Mo atom. The third branch of the pathway provides homocitrate synthesized by the condensation of acetyl coenzyme A and α-ketoglutarate, a reaction catalyzed by NifV (94).
The putative NifEN/NifH complex would integrate the building components provided by the three biosynthetic branches to complete the synthesis of FeMo-co in a redox-dependent reaction or series of reactions that also require Mg·ATP. The newly synthesized FeMo-cofactor is transferred to the cofactor deficient apo-NifDK protein to generate catalytically active NifDK. The transfer process is mediated by the nitrogenase accessory factor NafY, a non-nif metallochaperone that is thought to protect FeMo-co from degradation and to stabilize the FeMo-co deficient apo-NifDK (95, 96).
The nifQ gene product is an iron-sulfur protein known by genetic evidence to be involved in the incorporation of molybdenum into nitrogenase. NifQ is present in A. vinelandii cells grown in the presence of molybdenum and the absence of ammonium. At concentrations of molybdate in the medium around the nM range, nifQ mutants of A. vinelandii are impaired in molybdenum-dependent nitrogen fixation (97). It is significant that nifQ mutants are not defective in the activities of other molybdoenzymes (98) or the alternative V- or Fe-only-nitrogenases (85). Although nifQ mutant strains are not defective in molybdate uptake, it has been observed that they accumulate lower levels of molybdenum than the wild-type strain. The nifQ phenotype is suppressed by increasing the molybdate concentrations to μM levels or by adding excess cysteine to the medium, suggesting that the reaction catalyzed by NifQ might also occur non-enzymatically when the levels of the reactants are high. Similar nifQ phenotype and suppression profiles have been observed in K. pneumoniae (99, 100).
All nitrogen-fixing Proteobacteria –except some species of Rhizobia- contain NifQ homologues. Amino acid sequence alignments of NifQ proteins show a conserved Cx4Cx2Cx5C motif at the C-terminus of the protein that could be capable of coordinating the [Fe-S] cluster of NifQ. The native molecular weight of A. vinelandii NifQ (25.7 kDa) is similar to the weight deduced from the nifQ sequence (19.7 kDa), indicating a monomeric structure and precluding the possibility of having one [Fe-S] cluster coordinated by two NifQ subunits (93).
Recent biochemical and EPR spectroscopic evidence has shown that NifQ carries a metal cluster comprising a [MoFe3S4] core, and that the presence of this metal cluster in NifQ is correlated with its ability to support in vitro FeMo-co synthesis (93). As-isolated NifQ exhibits EPR properties similar to those of [Fe3-S4]+-containing proteins, whereas the EPR signals of reduced NifQ resemble those of [MoFe3S4] metal clusters prepared synthetically. Metal analysis, and the observation that [Fe3-S4] to [MoFe3S4] cluster conversion could be achieved in vitro by incubating NifQ with molybdate and sulfide under reducing conditions, confirmed that each NifQ molecule contains a single [Fe-S] cluster with the capability to carry a Mo atom.
Molybdate must undergo at least three chemical transformations before being incorporated into FeMo-co: replacement of O-ligands by S ligands, reduction of molybdenum from the MoVI oxidation state to the MoIV state found in the cofactor (101, 102), and insertion of molybdenum into an [Fe-S] cluster environment. The [Fe3S4] cluster observed in as-isolated NifQ could be the site for molybdenum binding and reduction. The reduced [Fe3-S4]0 clusters are known to be able to coordinate heterometals to complete [MFe3-S4] clusters in reversible equilibria (103). An attractive hypothesis is that NifQ would have its role in molybdenum trafficking through a cyclic process of molybdenum binding to the [Fe3S4] cluster by reductive coupling to molybdate and molybdenum releasing from the [MoFe3S4] cluster in response to an oxidation event (Figure 4). Consistently, EXAFS analysis strongly suggests that the molybdenum within NifQ is in the (IV) oxidation state (George, Hernandez and Rubio, unpublished results).
The [MoFe3S4]-loaded form of NifQ serves as specific molybdenum donor for the NifEN/NifH proteins during FeMo-co biosynthesis. A puzzling fact is that molybdate too can serve as a Mo source for FeMo-co biosynthesis in vitro. However, genetic evidence indicates that NifQ has an essential role when the levels of available molybdenum are in a physiological range. Mobilization of molybdenum from NifQ requires the simultaneous participation of NifH and NifEN, suggesting that NifQ would be the physiological molybdenum donor to a putative NifEN/NifH complex.
The NifEN protein, as isolated from a strain lacking nifH, contains substoichiometric amounts of molybdenum (104). The lack of NifH impedes the final steps of FeMo-co biosynthesis causing accumulation of biosynthetic intermediates within the NifEN scaffold. The presence of molybdenum in NifEN has been a rather contentious finding because it appears to depend on the purification method used to isolate this protein (104, 105). However, two independent observations support that the molybdenum within NifEN is indeed relevant to FeMo-co synthesis: (i) the purified preparations of NifEN served as a sole molybdenum source for the in vitro FeMo-co biosynthesis assay in a defined reaction mixture containing only purified components (104). (ii) The EXAFS analysis showed that molybdenum in NifEN is part of a [MoFe3S3+n] cluster but not adventitiously bound molybdate (106).
Although the quality of EXAFS data was not high enough as to discard the possibility of the [MoFe3S3+n] being part of a larger [Mo-Fe-S] cluster, the lack of apparent long-range (5Å) metal interactions supports the hypothesis that this cluster is in fact a [MoFe3S4] cubane. In such a case, this cluster would be very similar to the one carried by NifQ, and it is possible to envision at least three biosynthetic origins. First, NifEN contains a "receiving" [Fe3S4] cluster that accepts a reduced Mo atom or a Mo-S molecule extracted from the [MoFe3S4] cluster of NifQ, which would act as a donor. This would be a redox dependent metal transfer similar to the ones reported to occur in reduced [Fe3-S4]0 clusters (103). The observation of EPR signals that show similarity to signals from [Fe3S4]+-containing proteins in preparations of NifEN lacking molybdenum is consistent with this scenario (105, 107). Second, the complete [MoFe3S4] cluster is transferred from NifQ to NifEN. Transfer of complete [Fe4S4] clusters from scaffold proteins to target apo-proteins is widespread in nature (108). Third, the NifEN [MoFe3S4] cluster could have its origin in the reductive coupling of molybdate into a "receiving" [Fe3S4] cluster in a NifQ-independent pathway. It is important to note that the NifEN was isolated from a strain that contains NifQ but lacks NifH, and that molybdenum transfer from NifQ to NifEN is not efficient in the absence of NifH (93). This pathway could be responsible for the suppression of the nifQ mutant phenotype observed by increasing the concentration of molybdate or cysteine in the medium (99, 100). In any case, the [MoFe3S3+n] cluster of NifEN has been shown to be a molybdenum donor for the VK-cluster, which is the other FeMo-co biosynthetic intermediate carried by NifEN (104).
NifH too has been proposed to serve as the entry point for molybdenum incorporation into the FeMo-co biosynthetic pathway. 99Mo radiolabeling experiments showed the incorporation of 99Mo into NifH in FeMo-co biosynthesis reaction mixtures containing purified NifEN, NifH, NifB-co and Mg·ATP (109). However, it should be noted that incorporation of 99Mo occurred also in NifEN, and that the presence of all components (NifEN, NifH, NifB-co and Mg·ATP) were required for 99Mo incorporation into both NifEN and NifH. Similar results were observed by EXAFS analysis of FeMo-co precursors associated with NifH after in vitro FeMo-co biosynthesis reactions (110). Since the presence of the [MoFe3S3+n] cluster of NifEN is not absolutely dependent on NifH (104), a possible explanation for the role of NifH would be that its activity is required to mobilize molybdenum from the [MoFe3S3+n] cluster into the NifB-co-derived VK-cluster within the NifEN protein thus generating a molybdenum-containing FeMo-co biosynthetic intermediate.
The finding that the [Fe-S] cluster of NifQ was involved in the sequestration and delivery of molybdenum during FeMo-co synthesis has triggered many questions (111). Perhaps, the most intriguing one is how would NifQ exert all the biochemical transformations upon molybdenum that are required for such trafficking. A molybdate-reducing system that replaces Mo-O bonds by Mo-S bonds, reduces molybdenum, and inserts the heterometal into the [Fe3-S4] cluster of NifQ must exist in order to generate a [MoFe3S4] cluster. We hypothesize that NifQ is the central protein of an enzymatic system with molybdate reductase activity. Adjacent to nifQ in the minor nif region are genes encoding a ferredoxin (Avin51020), NifO (Avin51030), a putative rhodanase (Avin51050), and a monothiol glutaredoxin (Avin51060).
Some glutaredoxins have been described to be involved in [Fe-S] cluster assembly (112). Thus, it is possible that the nif glutaredoxin has a role in the formation of the [Fe3-S4] cluster of NifQ, which serves as scaffold for molybdenum binding. Rhodanases have sulfur transferase activity and have been shown to direct the reconstitution of [Fe-S] clusters in ferredoxins and NifH (113, 114). Thus, it is possible that the nif rhodanase could be involved in the synthesis of the [Fe3-S4] cluster of NifQ. The generation of the [MoFe3S4] cluster could be achieved by reductive coupling of molybdate to the [Fe3-S4] cluster. The nif-specific ferredoxin could be involved in electron donation to reduce the [Fe3-S4] cluster of NifQ. Finally, the NifO protein is similar to the arsenate reductase ArsC, which uses reduced glutathione to convert arsenate to arsenite. Whether or not NifO could be involved in molybdate reduction remains open.
We thank Carlos Echavarri-Erasun for helping in figure preparation.
†This work was supported by ERC Starting Grant 205442 (L.M.R.), by Midwestern University Intramural Funds (J.A.H.), and by NIH GM-65440 grant (S.J.G.).
1FeMo-co, iron-molybdenum cofactor; FeV-co, iron-vanadium cofactor; FeFe-co, iron-only cofactor; Mo-co, molybdenum cofactor; NifB-co, NifB-cofactor; VK-cluster, Vinod K. Shah cluster; nif, genes encoding proteins involved in nitrogen fixation; EPR, electron paramagnetic resonance; EXAFS, extended X-ray absorption fine spectroscopy; ENDOR, electron nuclear double resonance.
2The NifDK component of nitrogenase is also referred to as dinitrogenase, component I, or MoFe protein. The NifH component is also referred to as dinitrogenase reductase, component II, or Fe protein.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers. |


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'Showy' Lewisia can live for ages
If you were going to grow a California native that blooms with abandon, you could always try Lewisia.
It is one of my favorite succulent plants, found in the wild on the highest north-facing cliffs in the West. The danger for me is that Lewisia cotyledon is highly collectible, with hybrids available in every color you can imagine.
Lewisia cotyledon is an alpine plant, but don't let that scare you off. Alpines are tolerant of harsh conditions, hugging the rocks to stay warm and preserve moisture, and blooming like crazy in places where summers are short.
In the lowlands, Lewisia happily blooms on and off all year.
"Lewisias are very showy plants," said John Bishop, horticulturist for Sherman Library & Gardens in Newport Beach.
"Here, we treat them as annuals," he said, letting them do their thing until midsummer, then replacing them when they are done for the year.
But you don't have to. These succulent perennials can live for ages, given the right conditions. Water, soil and location are keys to succeeding with Lewisia.
Beginning with water, remember these plants hail from harsh mountaintops. You will want to water regularly, but that water needs to pass through the pot as quickly as possible.
And regularly doesn't mean wet. Lewisias easily rot if they are overwatered.
"Watch the weather," Bishop said. "Let the pots dry out slightly before you water again."
Planting in deep clay pots is best, since these plants are tap-rooted in maturity – meaning the roots are carrot-like.
"Don't break up the roots when you transplant," Bishop said.
You can make your own fast-draining potting soil by adding pumice and perlite to your potting mix. Bishop throws in a pinch of Osmocote 14-14-14.
"We grow Lewisias in full sun along the coast," he said. Inland, morning sun and afternoon shade protect the succulent leaves from sunburn.
Lewisia cotyledon is difficult to start from seed, so look for plants at nurseries that carry Monrovia and Native Sons, or shop online at anniesannuals.com.
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Bucket list- some great ideas for what to do with $1 buckets from Target's Dollar Spot or Dollar Tree Store
The ultimate bucket list of things to do in Ohio
Travel to Michigan to visit Mackinac Island and do all these things on our Mackinac Island Bucket List.
You Must Visit These 13 Bizarre Route 66 Attractions In Arizona Sedona Prescott Phoenix Scottsdale Flagstaff AZ Tempe Mesa Gilbert Payson Arizona Bucket List Travel Road Trip
5 Places for Family Fun in Charlotte, North Carolina | CosmosMariners.com
From the mountains to the coast, we've got a ton of ways you can get to know the Tarheel State. #NorthCarolina
Check out this list of family friendly things do see and do in Florida. Must add them to your bucket list!
101 Things to Do in Seattle Washington { click through to get the printable version } - the Ultimate Seattle Bucket List - from the popular spots everyone has to do at least once to the spots a little more off the beaten path. // localadventurer.com
Virginia is rich in history. This Virginia Bucket List is packed with 20 must visit sites and attractions throughout the state plus city specific guides.
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BCCI president N Srinivasan refused to talk about the issue concerning IPL franchise Deccan Chargers as the matter was still in court. However, he mentioned that the Board can still advertise for a new team to be added to the Indian Premier League.
"I cannot comment on the matter (of Deccan Chargers) as it is currently sub-judice. I'm informed that the court may issue an order soon," Srinivasan told reporters after the Board's 83rd AGM.
"But as far as the Board is concerned, it is at liberty to advertise for another franchise. But that's a decision to be taken. If we have to go with nine teams, so be it," he added.
The Bombay High Court appointed retired Supreme Court Judge C K Thakkar as arbitrator to bring about a resolution to the dispute between BCCI and Deccan within three months. The issue between BCCI and Deccan Chronicles Holdings Ltd (DCHL) arose when the the Board decided to terminate Deccan Chargers from IPL.
Justice S J Kathawala ordered appointment of Justice Thakkar to mediate after the parties failed to come up with mutually acceptable names of arbitrators.
The DCHL had also made a plea to the court to stay BCCI's decision to terminate the franchise but the court chose to reserve the order.
The court further ordered DCHL to give Rs. 100 crore bank guarantee to BCCI within 10 days after it passes an order on the former's petition seeking a stay on the termination.
We are at liberty to advertise for new franchise: Srinivasan
Cricbuzz Staff in Bangalore •
N Srinivasan said the Board can still advertise for a new team to be added to the IPL. © Cricbuzz
© Cricbuzz (with inputs from agencies)
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This dish is representative of my area of the country, and particularly the Texas-Louisiana border. The okra, corn and Creole seasonings are all popular here.
Classic Southern Recipes
- 1 cup chopped green pepper
- 1/2 cup chopped onion
- 3 tablespoons vegetable oil
- 2 cups fresh or frozen corn or 1 can (15-1/4 ounces) whole-kernel corn
- 1-1/2 cups fresh sliced okra or 1 package (16 ounces) frozen okra
- 3 medium tomatoes, peeled and chopped (1-1/2 cups)
- 1 tablespoon tomato paste
- 1/4 teaspoon dried thyme
- Salt to taste
- 1/4 teaspoon coarsely ground pepper
- 1/2 teaspoon hot pepper sauce, optional
- In a large skillet, saute green pepper and onion in oil until tender. Add corn and okra; cook over medium heat for 10 minutes, stirring occasionally.
- Stir in the tomatoes, tomato paste, thyme, salt, pepper and pepper sauce if desired. Cover and simmer for 3-5 minutes, stirring occasionally. Yield: 4-6 servings.
Originally published as Corn Okra Creole in Country Extra
July 1990, p45
Reviews for Corn Okra Creole
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Reviewed Oct. 21, 2015
"We found this to be very bland. Not sure how to give the dish more flavor, but I'm in no way inclined to make it again to find out."
Reviewed Aug. 26, 2010
"I've never liked okra before, but this dish turned out really good! Leftovers reheat well."


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*images courtesy of lucy laucht, leta austin foster, tim babber, my cup of te
Wednesday, February 27, 2013
*images courtesy of lucy laucht, leta austin foster, tim babber, my cup of te
5 comments:
- Siena in Style said...
that flower paper is gorgeous!!love the shirt too
kisses from Milano
http://sienastyle.blogspot.it/ - February 27, 2013 at 12:49 PM
- simplymodernhome.com said...
Congrats on feeling better!
Beautiful photos today!
simplymodernhome.com - February 27, 2013 at 2:41 PM
- Natalie {Designer Bags and Dirty Diapers} said...
Thanks for the shout out, Shannon! I hope you managed to score a few goodies at Target- is it sad it is one of my favorite places to shop? Have a great weekend!
- March 1, 2013 at 11:56 AM
- BaseWallpaper wall said...
nice wallpaper
basewallpaper.com - March 30, 2013 at 3:33 PM
- Cheryl said...
The floral wallpaper is so beautiful. Do you know who makes it or the pattern name? Thanks.
- July 13, 2013 at 12:48 AM
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text: What It's Like To Stand Inside an Imploding Stadium [Updated]Joel Johnson4/28/10 8:40pmFiled to: FootballCowboysDallasConstructionDestructionExplosionImplosionTexas StadiumTexas171EditPromoteShare to KinjaToggle Conversation toolsGo to permalinkWhen the Dallas Cowboys blew up Texas Stadium this week—punting accident—they had the good sense to put in a 3D camera rig inside. Click and drag to move the camera around. And turn up the sound. Boom!Reply171 repliesLeave a reply


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As an organization devoted to dialogue as the surest path to peace, the Ikeda Center values the interview format for engaging with old and new ideas and truths. Please visit our videos section for more interviews.
Mary Catherine Bateson is a cultural anthropologist and bestselling author. She explores "A Time for Active Wisdom." | |
Virginia Straus Benson is a Senior Research Fellow at the Ikeda Center. Here, she reflects on the Center's first ten years. | |
Janine Benyus is author of Biomimicry: Innovation Inspired by Nature. We can look to nature for creative solutions. | |
Carolyn Boyes-Watson is a scholar and practitioner of restorative justice, the subject of this conversation. | |
Robin Casarjian & Judith Thompson are leaders in the field of social healing. What is "the healing paradigm"? | |
Harvey Cox is Hollis Research Professor of Divinity at Harvard Divinity School. What is "the future of faith"? | |
Riane Eisler is a a cultural historian and bestselling author. This interview looks at the "Economics of Caring." | |
Benjamin Ferencz is the only surving prosecutor from the Nuremberg Trials. Here he urges: Law Not War! | |
Neva Goodwin is an economist at Tufts University. Here, she discusses "contextual economics." | |
Stephen Gould directs the ed. leadership program at Lesley University. Explored here: "The Music of Leadership." | |
David Hansen of Teachers College, Columbia University looks here at the moral dimensions of education. | |
Vincent Harding, influential activist and historian, calls for "Nonviolent Change of Revolutionary Depth." | |
Donna Hicks is the author of Dignity: Its Essential Role in Resolving Conflict, the topic of this 2014 conversation. | |
Victor Kazanjian is Executive Director of the United Religions Initiative. This interview is called "Education and the Middle Way." | |
Pam Kircher is a veteran hospice physician. This conversation is on "Humanizing Healthcare and the Process of Dying." | |
Paul Knitter is a leading theologian of religious pluralism. Explored here: ethics and the search for common ground. | |
Linda Lantieri is Director of the Inner Resilience Project. This 2002 piece is called "The Power of Holistic Education." | |
Bernard Lown is a cardiologist and co-winner of the 1985 Nobel Peace Prize for his work opposing nuclear war. | |
Ceasar McDowell is Professor of the Practice of Community Development at M.I.T. Here, he explains "Bg Democracy." | |
Mary Lee Morrison is the biographer of Elise Boulding. Discussed: "The Beauty and Strength of Peace Building." | |
Nel Noddings is one of our great philosophers of education. This piece is "To Reduce War You Must Understand It." | |
Gonzalo Obelleiro is a scholar of value-creating education. What are the moral dimensions of Soka education? | |
Steven Rockefeller, professor emeritus of religion at Middlebury College, discusses "Ethics and the Earth Charter." | |
Sir Joseph Rotblat founded the Pugwash Conferences, for which he was a co-recipient of the Nobel Peace Prize. | |
Peter Stearns is University Professor at George Mason University. This piece looks at his book Peace In World History. | |
Judith Thompson promotes social healing in countries riven by violence. Here she discusses "Compassion & Social Healing." | |
Robert Thurman of Columbia University explores the inherent educational aspects of Buddhism. | |
Tu Weiming, formerly of Harvard and now of Peking University, discusses the topic of "the dialogue of civilizations." | |
Nur Yalman (2002) is a cultural anthropologist at Harvard University. This 2002 interview looks at the post-9/11 world. | |
Nur Yalman (2009), co-author of A Passage To Peace with Daisaku Ikeda says that empathy makes us human. | |
Masao Yokota sat down for this 2013 discussion on the ideals that shaped the Center after our founding in 1993. |


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My candyland location sign! Love how it came out
candyland game board template
These Candy Land Directional Signs will shows us all the important places in the world of Candy Land.
Holiday Candyland Birthday Wonka Party Indoor/Outdoor Lollipop Decorations
Enter into Candyland through this lollipop and candy-covered arch! | Balloons by Tommy | #balloonsbytommy
DIY Giant Lollipops | DIY Party & Crafts
DIY JUMBO POPSICLE GARLAND ~ Made using pool noodles... Great for any outdoor Summer celebration or party
My husband made these out of cardboard for a candy land b-day theme :)
Willy Wonka's Candyland Wonderland Themed Party with So Many Cute Ideas via Kara's Party Ideas : Sweet Entrance
candy cane prop | Click the image to Supersize


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BioMathematics Initiative
The UPLB BioMathematics Initiative is a research endeavor that was set up in the year 2010 by the Institute of Mathematical Sciences and Physics (IMSP). It is spearheaded by a group of mathematics educators, researchers and students who work with people from other fields of study such as biology, biophysics, computer science, statistics, chemistry, environmental science and management, agriculture and forestry. |
Coding Theory and Cryptography At present, the cluster concentrates on the algebraic properties of block codes and convolutional codes over rings, the construction of convolutional codes from block codes, the derivation of certain distance bounds, and the structural properties of finite rings, particularly $F_{p^k} + u F_{p^k}$. |
Computational Physics
Computational Physics Laboratory (COMP) aims to be the leading computational research laboratory capable of solving real world problems. COMP uses mathematical models and numerical algorithms to model and simulate complex physical processes such as those occur in condensed matter physics, social and biological systems. |
Computer Hardware and Instrumentation Physics The Computer Hardware and Instrumentation Physics Laboratory (CHIP) aims to be known in the Philippines as the leading laboratory that develops and make instruments and systems for solving real-world problems. CHIP's main goal is to use the physics theory learned in creating world renowned instruments for different applications. |
Mathematics Education | |
Operations Research (OR) is the discipline of applying advanced analytical methods from mathematics, science, and engineering to help better decisions in planning ventures and analyzing options. Operations Research is characterized with its broad applicability by its interdisciplinary nature. As OR attempts to resolve the conflicts of interest among the components of the organization in a way that is best for the organization as a whole, the OR team needs to include individuals who collectively trained in mathematics, statistics and probability, economics, business administration, computer science, engineering and the physical sciences, the behavioral sciences and the special techniques of OR. |
Last Updated on Thursday, 06 March 2014 10:24


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Scott Stapp on God, and Why Creed are Like Led Zeppelin
Scott Stapp
care of Scottstapp.com
Love him or hate him, Scott Stapp has one of those over-the-top personas that seem part and parcel of being a rock star. Best known as the leader of Creed, Stapp's deep, bellowing vocals helped that band reach massive popular heights in the late '90s and early 2000s. During that time, Stapp became embroiled in various sexual and alcohol-related situations.
These days, however, Stapp is sober and highly spiritual. His second solo album, 2013's Proof of Life, has done well on Christian radio and Stapp remains very committed to a number of charities. Speaking from his home in Florida and in anticipation of Wednesday's show at the House of Blues, Stapp spoke with DC9 about how even his darkest days helped prepare him for the better man he would become.
It's been a while since you came through our area. Have your experiences here always been positive?
Dallas has always been awesome. The city has always been great to me. I remember a show there in 1997. I've had some of my best shows in Dallas all through the years. The fans there have continued to support me and every album I've done.
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Creed seemed to be quite a divisive band. It was always a love it or hate it proposition. The band was embraced by the public, but always got some very negative reviews. Why do you think that is?
I think that happens with bands that have tremendous success. You have people that love the band and you have people that hate it because it became instantly popular. They think that it is uncool to like the band. The same thing happened to Nickelback. It is something that happens in rock and roll. It happened to Led Zeppelin. When they released their first couple of albums, Rolling Stone said they were the worst band ever. Now, you will probably hear praise of Led Zeppelin as the god of rock and roll music. It's funny how things happen and turn around.
When you were young, you rebelled against religion and your Pentecostal father. Now, you've come full circle and embraced Christianity. What fostered that turnaround?
I definitely think that becoming a Christian and making that decision when I was young planted that seed in me. I decided to discover that truth for myself and find it later in life. I went out and learned the hard lessons that I was warned about in the church. It became that time. It was just in me since I was young and something I had never abandoned. I just wrestled with it. I had a lot of questions, something a lot of people do when they go off to college. In their 20s, they kind of want to figure out what they believe in. You have things you want to figure out. I had that conviction in my heart that I knew there was a God. That stayed with me.
You have the No. 1 record on the Christian radio charts. Could you ever have envisioned that when you were in Creed?
You know, at that point in time in my life, my dreams and my goals were much different. I was trying to distance myself from that in my professional life. I didn't know that the two could come together. Looking back at it now and being in this position now, it makes sense. Some of my favorite bands growing up were Christian rock bands. That was the only music I could listen to in my home. Looking at it now, with everything that has happened in my life, it makes sense. It's probably where I belong.
Did your embrace of Christianity coincide with your sobriety?
Oh no, I've always been a Christian. No matter what my struggles and doubts were, no matter what problems I was facing, I still was a Christian, I still believed in Christ. I had that faith. I didn't always live it. I wanted to experience things and learn the hard way. I wanted to figure things out myself. Being sober has definitely enabled me with clarity to finally assess my life and how I want to live it, what works and what doesn't. I was always a Christian. I think there is a misconception out there where people think that if you are a Christian, you are perfect. And I think there are a lot of Christians out there who would agree with me. It's definitely something to attempt to be better people and be more Christ like. We all have times in our lives where we don't necessarily live like we believe. We strive to be better. I went through that period and I am sure, at various levels, it will never be like it was in my 20s. I'll wander off and learn on this journey. I think that is a part of what a Christian is all about. For years, Christians and preachers in the '80s were put up on such pedestals. When they made mistakes, like every man does, it was a big black eye for the Christian community. I think it is sad when things happen that way. That is not how it should be represented. I think that Christianity and Christ came for those who are fully flawed. We are in need of a savior to help us navigate through life. That is exactly how I am and I think that is how the average Christian is as well.
You've given millions of dollars to various charities. It that about making amends for your past by walking the walk as a spiritual person?
Growing up as a kid, I was poor. My mom had to support three kids on her own with a $90 a week salary. We were on food stamps and welfare. I struggled to have a pair of tennis shoes. When I found some success and I had some money, I just felt that I wanted to help kids who were like me when I was little. Now, I know the church talks about giving and helping to take care of the poor and I am sure that's where the seed was planted in me. My initial desire, however, was because I had lived it; I was there. I wanted to do something for other kids who were like me.
The music of Creed continues to be used in movies and other formats decades after it was recorded. Why do you think it has resonated so well?
I can give you my opinion of why I think it has stood the test of time. I think, number one, it's kind of a symbol of rock and roll music. Rock and roll has a timeless appeal to it. That's part of Creed's influence upon classic rock music. It implants something in your DNA, a certain timelessness. We approached songwriting by sticking to a more traditional sound with more depth content-wise. One of the reasons that we have stood the test of time is that was our goal. People who didn't get us and may have been on the opposite side of the fence, those Creed haters. It's funny. I've seen so many stories of people who have reached a certain age and all of a sudden, they hear a song and it connected with them. It's kind of cool. Creed has been out on tour the last four years all over the world. The audience never gets old. The band is getting older, but the audience doesn't age. That is just a blessing to me and all of us. The music keeps identifying with a new generation.
Will there be another Creed album?
Right now, I want to concentrate on Proof of Life, my second solo effort. I am excited to have some success with the album. I've had some success in the Christian market and on classic rock radio. I am really excited about this record and what it means in my life and the story that it tells. I think it's where I am supposed to be right now. I think when the time comes and things are right, Creed is always there for all of us. When it is the right time and it feels right. But right now, the focus is on my solo work. I hope my record fills the gap for any Creed fans out there.
You've been through sex scandals and battles with alcohol. Looking back over those times, is there anything you'd want to change?
I will give you a yes and a no answer. In some respects, I wouldn't change anything. That's part of what makes you who you are: the embarrassments and the setbacks. They all had an impact on my life. Those things, good or bad, helped me get to where I am today. Of course, there are things that I wish could have stayed skeletons in my closet. I think all of us have things that we would like to keep buried, things that only we know about. Unfortunately for me, so much of what happened to me has been exposed. I had to learn from that. I had to wonder how to turn the bad into something positive. I asked God why he let some security guard steal a video tape of me and Kid Rock when I was 22 years old. Why did you allow that to happen? What purpose did that serve? Now, the way I look at it, it did serve a purpose to a bigger story of what God has done in my life. I know what it's like to live on both sides of the fence. It turned what looked like a mess into a message.
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Thursday, July 24, 2014
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For those asking how Jack has been the day after his gnarly ant bite situation...
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Wednesday, July 16, 2014
Jack got a fire ant bite on his finger today. Anyone else's kid ever react this way?
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Thursday, July 10, 2014
My dad. The most rockin' dude with his neon and big ole camcorder. #tbt
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If you're going to blow your diet, then this is the way to do it!
Daily Archives: July 20, 2011
To join Woody and Buzz
I already have Woody and Buzz hanging out at my desk at home; time for Jessie and Bullseye to join them 🙂
a Dowtown Disney MUST shopportunity
The Nieceling has never been to Tren-D. I know, shocking. Fortunately, Auntie Tink is here to remedy the situation.
Now entering Heaven
Going to eat at Downtown Disney
On our way home, we'll stop in Downtown Disney
This was just a quick trip to pick up the Nieceling and bring her back to SW Florida with me. But that won't stop us from dining with the Mouse 🙂
Where in Universal Orlando are we?
I will give you a hint – it's a bit windy! 😉
Park hopping at Universal Orlando
We want to do something at the Studios before we head back to Southwest Florida. I thik it will be The Mummy – single riders line, of course!
Mmmm, (butter) beer
Mine is the frosty variety. We have sought shade at a table behind the Three Broomsticks. I can't believe I'm in a theme park in July!
On line for the Forbidden Journey
It's going quickly, and we will do single rider line once inside
New baby born at Universal IOA
Baby velociraptor hatched before our eyes LOL
Up-shot in the rotunda
Imagine being a small forest creature, looking up in terror at the underside of a dinosaur's jaw!
Obey the signs at IOA
No pushing sticks into ground with foot!
Cool-off shopportunity
Going to cool off inside while we wait to get into Wizarding World of Harry Potter
Harry Potter come-back time in one hour
They now have kiosks to get a ticket to come back and enter Hogsmeade at Islands Of Adventure. It looks VERY crowdwd in there!
Birds find the oddest places to nest
Check the nostrils!
Heading into Islands of Adventure
The barkers are telling everyone "Harry Potter to the right" – sure, ruin the "secret" shortcut LOL
Breakfast view at City Walk
We're dining on total crap food at Starbucks but it's totall awesome 🙂
Do not lose your car at Universal
It's embarrassing! Shout out to my sistas….
Good morning, Orlando!
I like this hotel. It is right outside Universal Orlando and just across the street from Wet N Wild. There's an IHOP next door and a Walgreens on the next corner. Also, you get an olfactory treat from "Eau du Four Points" wafting through the lobby, same as when you go to the Walt Disney World Swan and you get "Eau du Swan".
Finally, the hotel is free using Starwood Preferred Guest points. Free is good 🙂 If you want to know how you can stay free too, send me an email BellTinkR at gmail dot com.


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Java Draw Created Jun 06, 2010 470 x 338px Unless noted Copyright 2010 shiver.
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shadowdenigma Jul 09, 2010
Captain Planet, he's a hero, going to take pollution down to zero! LOL! Great work on him :3
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