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West Bridgford, England NG2 7DE, สหราชอาณาจักร
Sarah & Rob were great hosts - friendly and accessible without being intrusive. Thanks very much for having me
Andy2016-10-19T00:00:00ZLovely, generous couple with a well furnished, clean, comfortable warm room in a cool house and an especially nice big kitchen for cooking and eating. The room's right next to a nice loo/shower for guests, all on the top floor that feels like your own flat. They also really helped me figure out something tough and person: a testament to their generosity. I hope future airbnb experiences are this positive!
Griffith2016-10-21T00:00:00ZI was in the room with two single beds along with a friend. The room was organised and clean, as was the bathroom. We pretty much had the floor to ourselves. Sarah was welcoming and approachable. She gave us the main information regarding the flat and was kind enough to trust us by letting us use her room in peace. Sarah was very helpful when we needed assistance. Overall, a positive experience and I would highly recommend it.
Kleant2016-10-02T00:00:00ZOur stay was fantastic, Sarah was an excellent host who couldn't be any more helpful. There wasn't anything that was too much trouble. We felt at home from the moment we walked in. Our room was perfect for our needs, spacious and comfortable. We really couldn't have been looked after any better. If we were in the area again we would definitely look to book with Sarah. Thanks for your hospitality.
Dan2016-11-06T00:00:00ZThanks for letting me stay in your stunning house. The room was perfect the photos don't do it justice. Beautiful decor, clean and cosy. You provided more then I expected with a kettle, hairdryer, shampoo etc. I had the top floor I felt safe and had privacy. Sarah made me feel welcome and comfortable. The area was lovely, safe with plenty of bars and cafes. Good value for money.
Rajna 2016-09-11T00:00:00ZI still cannot believe how lucky I was when I booked at Sarah's place! Fantastic hosts, and fantastic apartment. Very well located, clean house and quiet area. Bus stop and several restaurants and bars so close. The perfect balance between hospitality and privacy, and a lot of help during my stay in their home, with minor details that make you feel like at an old friend's house. Absolutely recommendable. Thanks Sarah!!
Sergio2016-06-14T00:00:00ZWe had a great stay with Sarah and Rob. They were very welcoming, hospitable and flexible allowing us to make the best of our plans in West Bridgford. The room had all we needed and it was good to be self contained on the top floor. Excellent value for money and we will definitely stay again when returning to Nottingham. Thank you!
Elle2016-09-18T00:00:00ZHad a lovely night's stay. Rob was very welcoming, & the room was all I expected in a very nice area of Nottingham.
West Bridgford, Nottinghamshire NG2 7NA, สหราชอาณาจักร
Siobhan is an excellent host. The flat was impeccably clean. She had a folder with information regarding activities in Nottingham. Always helpful with advice regarding directions to avoid the traffic. I would surely return if I am visiting Nottingham. Thank you.
Harish2016-12-24T00:00:00ZThe house was very clean and welcoming. Siobhan was on time and good at communicating with us. Nice comfy bed and lovely natural light coming into the room in the morning!
Ryan2016-10-09T00:00:00ZGreat communication. Lovely location and as described, clean tidy and comfortable Siobhan is a lovely and welcoming host.
Steve 2016-09-11T00:00:00ZSiobhan handed us the keys early as she was in which was a big help and was very friendly and let us know when the room was ready. Bed was extremely comfy, flat was really nice and in a great location. No hassle parking and only 5 minute taxi from centre.
Charlie2016-10-30T00:00:00ZSiobhan was very friendly and took time to answer all the questions I had about how to walk into town and where to go when I got there. We had a good natter when I arrived over a cup of tea. Room was exactly as described and there was nothing I could criticise. I wouldn't hesitate to stay again.
William Denis2016-10-02T00:00:00ZSiobhan is a very welcoming and friendly host who did everything she could to make my stay a good experience. Her home is comfortable with excellent facilities and the location is perfect if you are looking for peace and quiet, but also near enough to local amenities and the city beyond. I would not hesitate in saying here again.
thomas2016-10-03T00:00:00ZBrilliant place to stay. Very comfortable, clean and spacious bedroom and living area, a friendly welcoming host, and is close to West Bridgford and Nottingham. Would not hesitate in staying again or recommending to anyone!
Alex2016-10-07T00:00:00ZSiobhan was a very accommodating host with her apartment being extremely convenient for Nottingham City Centre, albeit in a quiet peaceful location just out of town. The listing was a very accurate reflection of the property, so thank you so much for accommodating me.
West Bridgford, England NG2 5EG, สหราชอาณาจักร
Very welcoming, lovely apartment and the attention to detail such as his and her deodorants were a great touch. Will definately recommend and will definately book with Dan again
Sharon2016-07-18T00:00:00ZDan was a brilliant host, very quick to answer emails before and during our stay, and welcoming on arrival. The flat is light and airy, clean and very comfortable with everything we needed. The large towels were a bonus! Thanks Dan, we hope to be back next time we are in Nottingham.
ALISON2016-08-17T00:00:00ZGreat annex in studio-apartment style. Well equipped and quality fittings. Dan was responsive and helpful, and West Bridgford is a good place to stay in itself.
Christopher2016-09-12T00:00:00ZDan is an excellent host and we like the private en-suite room as well as the garage for our baggage storage. West Bridgford is a nice small town and Dan's place is only 10 minutes' walk from city centre and 15 minutes' drive from downtown Nottingham. We'll definitely recommend Dan's place to our relatives and friends, especially those who need to stay around Nottingham.
Charles2016-09-24T00:00:00ZDan was extremely communicative, helpful and flexible. Unfortunately we had to cancel at the last minute, and he was very understanding.
Jamie2016-04-03T00:00:00ZDan's place was extremely comfortable and great to stay in for a long weekend. I had some work to do so having the use of the (beautiful) desk was ideal. Dan's is the perfect location for getting into Nottingham city centre or easily walkable from West Bridgford shops and the river. Dan was the perfect host and extremely welcoming and accommodating, would recommend to anyone!
Harriet2016-12-19T00:00:00ZThis was our first time to Nottingham/West Bridport and the studio was located about a 10-15 minute drive from Nottingham city centre which was perfect for us. The studio is exactly as described, it is very cosy and had some very nice touches... we even enjoyed a few games of Jenga on our very very rainy evening! Dan had provided very clear instructions prior to arrival which meant everything went smoothly, he ensured we had everything we needed once we arrived which was much appreciated. If visiting this area again we'd absolutely stay here again!
Odette2016-10-03T00:00:00ZDan is a very affable host and the room is spot on! Beats the hell out of a hotel room! :)
West Bridgford, Nottinghamshire NG2 7AA, สหราชอาณาจักร
West Bridgford, England NG2 5BU, สหราชอาณาจักร
Christina and Nigel made us feel very welcome from the moment we arrived. The lovely guest room was exactly as described. The bed was very comfortable and the room was spotless. There were tea and coffee making facilities and delicious chocolate chip cookies. The breakfast was amazing - fresh fruit salad, yoghurts, cereals and a full English breakfast. Christina has great attention to detail and really thinks of everything. We were there to visit the nearby National Water Sports Centre but we also had lunch and evening drinks in West Bridgford where we found a large choice of restaurants and bars. We would not hesitate to recommend and look forward to staying again the next time we are visiting the area.
Sue2016-07-24T00:00:00ZChristina and her husband were absolutely fantastic hosts. The room and ensuite bathroom were very stylish and spotlessly clean. The bed was really comfy and there were good tea and coffee facilities in the room with yummy biscuits (although Christina also kindly made me a cup of tea on arrival anyway). Christina made a great breakfast, with fruit and yogurt, and was happy to cook whatever cooked breakfast items we wanted. The house is set in a beautiful location with plenty of parking and a short walk away from the centre of West Bridgford. We would highly recommend staying with Christina and her husband. You really can't beat it for value!
Sam2016-07-11T00:00:00ZEverything was exactly as described, and it was an extremely comfortable stay with a lovely breakfast. Highly recommended! Many thanks!
Charlotte2016-12-16T00:00:00ZChristina & Nigel made me feel very welcome. The facilities are exactly as described - if not better ! If you are ever in need of accommodation in Nottingham/ West Bridgford area, be it for the cricket, football, tennis or anything else, head to Christina and Nigel's home - you won't regret it .
Janet2016-06-16T00:00:00ZA great place to stay, the bed and bedding were so comfortable and the bedroom and en-suite great, everything you could possibly need and more. Wonderful hosts with a wonderful home, who were most welcoming. Tea and the most amazing ingredients chocolate chips cookies on arrival and a fabulous breakfast catered to our needs.
Kathryn2016-12-12T00:00:00ZChristina was a brilliant host. As it was the hottest day of the year she had thought of everything to make my stay comfortable. Extras that you would not get in a 5 star hotel.
Gay2016-07-22T00:00:00ZChristina responded promptly and courteously to my messages, while arranging our stay. We were given a very warm and friendly welcome on arrival. The accommodation is exactly as described on the Airbnb website - stylish, beautifully appointed, and immaculately clean. If anything, the bedroom and en suite were even more luxurious than we had hoped for. Christina and her husband made us feel so much 'at home' in their home, really lovely hosts. They have thought of everything, down to the last detail, to give guests a very special stay. Christina provided us with a superb breakfast, plenty of choice, beautifully presented and of high quality. The house is in a very quiet residential street, so a peaceful stay is guaranteed. We would certainly highly recommend this host and her accommodation, and will definitely be back.
Anne2016-04-05T00:00:00ZThank you so much Christina and Nigel for a lovely stay. We felt welcome the moment we stepped in the door. The room was very comfortable and breakfast was fantastic! We wouldn't hesitate to stay again the next time we are in Nottingham. Amanda and Joe x
West Bridgford, Nottinghamshire NG2 5AP, สหราชอาณาจักร
West Bridgford, England NG2, สหราชอาณาจักร
My stay was very comfortable. I enjoyed meeting Fernando and Patricia - they are wonderful hosts and made me feel at home instantly!
Sergio2017-01-12T00:00:00ZLovely people, great location and very clean and comfortable.
Suzanne2016-09-23T00:00:00ZIt was very nice to stay at Patricia's home. I will definitely recommend them. All the best.
Vikram R2016-06-21T00:00:00ZI can recommend staying with Patricia and Fernando who are warm, friendly and easy going. I enjoyed our conversations as they have both lead interesting lives, whilst on the other hand they respected my privacy and the need for me to do my own thing. The room was perfect with all the facilities I needed as well as being clean and tidy. The location for me was perfect, but for anyone visiting it was within reach of transport links to the city as well as excellent shops and restaurants nearby. I look forward to staying here again the next time I'm in Nottingham.
thomas2016-09-19T00:00:00ZI had a great time here; top location with very welcoming hosts in a clean and cosy apartment.
Will2016-07-06T00:00:00ZCommunication was straightforward with a mobile contact number given out. Welcome was warm and friendly and the room comfortable. Shared facilities were clean, tidy and well maintained. Tea and coffee were made available. The room was adequate but could be improved by better curtaining to block out the street lights. Offered ear plugs presumably to blank out traffic noise or sound from adjoining flats.
Donald2016-09-21T00:00:00ZDelightful people who made me feel extremely welcome and comfortable. The room was clean and lovely and the area was beautiful. I will definitely stay here again if I come back to Nottingham.
Katherine2016-07-11T00:00:00ZPatricia and Fernando were very welcoming and friendly. They invited me to join them for some Venezuelian food which was very nice. The room was clean and the bed very comfy. Love the flat. Will definitely stay with them again
West Bridgford, NG2 7PS, สหราชอาณาจักร
We really enjoyed our stay with Thomas, who was extremely friendly and welcoming. He has a very beautiful home, and we had everything we needed in our room.
Rosalind2016-12-08T00:00:00ZThomas's house is really nice, in a quiet street but with easy access to West Bridgford and Nottingham city centre. The room has everything you need and Thomas has left some helpful notes about the local area. A great place to stay for a night out in Nottingham!
Dave2017-01-01T00:00:00ZVery friendly and helpful host. Stunning house, extremely comfortable and quiet neighbourhood. Anyone who like cats will adore Casper, I know I did.
Martin2016-12-28T00:00:00ZMade me feel very welcome, the house was lovely and in a pleasant area. Was even provided with a folder of useful information about the attractions of Nottingham.
Sam2016-10-26T00:00:00ZFelt very welcome in this beautiful home! Thomas and the cat Casper we're great hosts and ensured we were greeted when we returned to the house. Lovely place and very comfortable bed. Room was light and enough space for the two of us staying there! Thank you :)
Lauren2016-10-10T00:00:00ZThomas was very friendly. The room was nice and clean. Nice location, beautiful house and lovely cat!
Tatiana2016-10-05T00:00:00ZThomas is a very friendly and welcoming host who provided clear instructions to his place. The house is spacious, light and clean. It is a 25 minute walk to town centre across the RiverTyne and along the canal. The room is very basic, especially if you stayed 5 nights as I did. I have suggested he provides coat hangers, a bedside lamp, a chest of drawers and a blanket under the bedsheet. There is no curtain in the room, so be prepared for an early rise in the summer months. Thomas is a strict vegan, so self catering can be a challenge to non-vegans (no ready meals!). I made the mistake of importing Alpen for my breakfast, which apparently has animal products in it. Who would have known! All in all a positive experience, which could be made better with a little effort.
Karen 2016-10-15T00:00:00ZLovely place, friendly host, excellent location and smashing breakfast!
West Bridgford, Nottinghamshire NG2 7NA, สหราชอาณาจักร
A lovely spacious room with a comfortable bed and sofa. The wet room shower is great. A very convenient location and an easy going and friendly host. A great place to stay.
West Bridgford, England, สหราชอาณาจักร
West Bridgford, England NG2 6AH, สหราชอาณาจักร
Claire communicated brilliantly before our visit and she was a very welcoming host. Her house is exemplary, very clean, in great condition and with excellent facilities including sky sport and a parking spot directly outside the house.The house is in a quiet cul-de-sac but very close to the cricket ground and just a short walk from the shops restaurants and pubs of West Bridgeford town centre. We couldn't want for a better AirBnB experience, highly recommended!
Graham2015-08-11T00:00:00ZAbsolutely fabulous beautiful fantastic amazing classy brilliant I can just go on and on about nick and Claire and there beautiful house this was our first experience with airbnb We all loved it my kids fell in love with Claire and nicks house we did not want to come back the location of the house was so perfect in a great area of Nottingham everything was close just so perfect thank you so much nick and Claire you both amazing people I wish you all the best for the future and thanks so much Clair's mum a lovely beautiful elegant lady thanks ... hope to come back to your house again ❤❤
Muhammad Akaash2016-08-03T00:00:00ZWe stayed at Claire & Nick's place while visiting family in Nottingham. Their home is lovely & just as advertised. The house was comfortable & clean with everything we needed for our stay (including a cot which they specially organised for our baby). After a long journey we arrived & let ourselves in easily. The breakfast pack left for us was a very welcome addition given our late arrival time & 3 hungry little boys the following morning! Communication with Claire & Nick was clear, quick & hassle-free. The house is located in a quiet street close to all the amenities you might need with great access to the centre of Nottingham. We will definitely stay again in the future.
Clare2016-11-01T00:00:00ZClaire and Nick's place was perfect for our stay. They were extremely helpful and when we left something there at the end of our stay they contacted us and offered to post it - great!
Josh2017-02-04T00:00:00ZSehr schöne und gemütliche Unterkuft
Sinisa2016-10-13T00:00:00ZClaire met us at the property and was very welcoming and friendly. We had a great weekend in her lovely house. The house was very comfortable and immaculate and had everything that we needed for 6 of us. It was in a quiet street but near to all facilities in West Bridgford. A great location for a weekend's stay. Would certainly recommend Claire's house to other potential visitors.
Gillian2016-12-06T00:00:00ZWe were made to feel very welcome on Claire's beautiful home. From start to in is hit was perfect. Hosts, location, cleanliness, amenities in the house, local attractions, nothing could be improved on . One of the best Airbnb's we have stayed at. Great communication, charming hosts and great value for money.
Alicia2016-07-05T00:00:00ZA lovely well located home which we would recommend to groups or a family. Great location and lovely cosy home,
West Bridgford, Nottinghamshire NG2 5FU, สหราชอาณาจักร
Katie's flat was exactly as described and more! It was absolutely stunning and completely spotless when we arrived! Prior to arriving, Katie responded to emails/questions immediately which of course put me at ease having booked a flat abroad (and my first Airbnb flat!) The location was perfect for us being in West Bridgford and just a short walk to the shops, restaurants, and cafes. As my husband and I travel frequently to the UK to visit family, staying in a hotel with parking fees is quite pricey so I must say that we really enjoyed the secure, assigned parking space we had at Katie's flat. Thank you so much for providing us with our home away from home during the holiday season!
Erin2016-01-04T00:00:00ZKatie made us feel very welcome and indeed we discovered that my brother and her were already acquainted. The apartment was in a perfect location and allowed us to visit family and get around the place easily with good public transport and easy walking distance to the town. There were some problems in that the Internet and also the washing machine did not work on arrival but the problems were handled and sorted out quickly by Katie. The cost of having to use a laundry though was an unexpected and very unwelcome additional cost. Our biggest issue though was that the bed was the most uncomfortable we have every slept on and as a result we actually slept on the couch each night. Also, the apartment appears to be clean but nobody has ever cleaned under any of the furniture or even cleaned cobwebs away from the lampshades. Overall we were happy but likely would look elsewhere in future.
Paul2014-09-15T00:00:00ZBoth me and my boyfriend enjoyed our stay very much. The flat we stayed in was in a perfect location. Very closed to the bars and restaurants. Only 5 minutes walk to the Trent river. Katie has been really helpful. We highly recommend it.
Penny2015-02-27T00:00:00ZKatie is a hospitality pro and it shows– she had exemplary attention to detail and provided very responsive and speedy communication as well as a great attention to detail in equipping the place. The apartment itself is very modern, bright, and well equipped. It was perfect for my needs as I needed temporary accommodation while starting a new job, and this had everything I needed, from an excellent kitchen with mini utensils to an iron and ironing board to a washer dryer to perfect Internet to a hairdryer. Seamless! It's worth noting that it scores well on those variables that you can't always be sure of with temporary housing: it has a good darkness, it is quiet, the bed is comfortable and the shower great. I would not hesitate in choosing this over a hotel even for short holidays as it represents very good value.
Bridget2015-09-22T00:00:00ZGreat apartment in a great and convenient location. Katie went out of her way to make me feel welcome and help me out (when I broke the key!!). Would highly recommend the place and the host! Everything you needed was there. Transport is close by and the shops just down the road.
kathy2014-11-06T00:00:00ZKatie was most welcoming. Lovely flat. Very convenient for restaurants, coffee shops and bars in West Bridgford. Good parking and easy to find. As a lady I particularly liked being on the second floor. Felt very safe and cozy. Will definitely use again. Five stars!
Rhiannon2015-12-24T00:00:00ZOur stay was perfect. The apartment was very comfortable and very well equipped. Katie was very helpful and available. She gave us lots of information and advice. Everything was great!
Veronique2016-03-30T00:00:00ZKatie was so helpful.
West Bridgford, England NG2 5HG, สหราชอาณาจักร
A very smart pad suitable for business, romantic or leisure stays. Extremely com fortable, clean and modern in a delightful house. Good parking. Close to town centre and facilities in general. We can thoroughly recommend it. The owners were as helpful as they could possibly be. We hope to stay again when we next visit or family.
Peter2016-12-28T00:00:00ZI had a wonderful stay in Becky and Alex's home. The room itself was lovely and spacious with everything I needed. If you want a private stay the room is equipped with a kettle and tv with loads of viewing choices, but I enjoyed spending most of my time downstairs chatting with the family. The family were so welcoming and kind, I was sad to leave! I would highly recommend for anyone looking to stay in West Bridgford.
West Bridgford, England NG2 5JR, สหราชอาณาจักร
Anna's house was very nice and the neighborhood was calm during my staying. I could park my car in the street and there was a big super market at walking distance. West Bridgeford has a very charming market street where you can find anything you want to eat or do some groceries. When I arrived at Anna's place she made me feel like home directly. The room and the bathroom was tidy. Anna and her son took the time to know me and we had dinner together.
Alain2016-04-23T00:00:00ZFascinating experience in Nottingham. The best accommodation in Nottingham. The house is handy to the Victoria center, very clean and quiet. most importantly Anna is very kind and helpful. I will definitely choose Ana's hose next time.
shuangquan2016-03-16T00:00:00ZClean, quiet and a comfortable bed. Anna is very friendly.
Matthew2016-07-10T00:00:00ZSuper hospitable host, beautiful, comfortable place in fantastic area of Nottingham. Easily accessible by public transport and walking distance to many nice places. Highly recommended.
Dovran2015-08-01T00:00:00ZSecond time staying with Anna, lovely as always.
Matthew2016-08-22T00:00:00ZI stayed in this lovely Victorian House for 2 nights. Anna is a very kind host who showed me some nice places in Nottingham. The room was very comfortable, large and cosy. Anna has a feeling for interior design and has many interesting things to say about books, art, glorious food and all the good things in life. So I would recommend everybody staying in Nottingham to book a room in this house.
Gilles2015-08-12T00:00:00ZAnna is a very friendly and welcoming host, keeping in touch before I arrived. The room was in her lovely Victorian villa and was very comfortable and quiet, ensuring a good nights sleep. Breakfast was provided, at a small additional cost, and there was plenty to choose from. This area in Nottingham is very quiet and there are plenty of restaurants and shops in the local vicinity. All in all, a very pleasant stay.
Robin & Emma2015-07-08T00:00:00ZThe house and the room itself are lovely - modern, very clean, light, airy, warm, quiet and beautifully decorated in relaxing colours. The room is spacious with a sink in it and the bed large and comfortable. There's no ensuite but the bathroom is in the room right next door, and the shower is amazing with a drench head. Anna was very friendly, helpful and quick to respond both when I was planning my trip and when I was there, offering things like cups of tea and help booking taxis. I was staying after a wedding party, and she said she would let me in if I was back by midnight or leave me a key if I was back late. Thanks very much Anna, very enjoyable all round!
West Bridgford, NG2 7AG, สหราชอาณาจักร
Thank you Paul for your flexibility on last-minute booking and my odd departure hour for a bank holiday. The guest rooms are on a separate floor with the bathroom and shower right next to them. Everything is tidy and the bed is extremely comfy. Thanks also for tiny little welcome surprise in the room. The house is in a great neighbourhood with restaurants and shops just down the street. It's a nice walk into town or to the National Water Sports Center along the river, but if you prefer, the bus stop and taxi stand are just a 2 minute walk away as well. It was a very pleasant stay. Thanks a lot Paul and Jo!
Bjoern2015-05-06T00:00:00ZGreat hosts, very nice property in a very nice area. 5 star and will return again if ever back in Nottingham.
Steve2015-10-22T00:00:00ZPaul made me feel very welcome. The house was lovely and the room was nicely made up for me.
Stine2015-12-04T00:00:00ZAnother great stay with Jo and Paul. I was made to feel very welcome and the room is really comfortable, as is the snug room. The street itself is really quiet and I slept very well. At the end of the road is A large number of both chain and independent restaurants, cafes and bars, and bus stops that made travel to and from the city centre really easy. A lovely place to stay with great hosts.
Julian2014-11-16T00:00:00ZI spent wonderful time at Paul's house. The location is strategic, West Bridgford is just 15 min walking to Nottingham city center. Bus stops are 2 minutes walk from the house. Great comfort in a typical and beautiful English house! The family made me feel very welcome! The room was so lovely! I really appreciate the time I spent with of Paul and Jo! No doubts about cleaning, highly recommend! Many thanks to Paul,Jo,Poppy and Charlie!
Francesco2015-12-09T00:00:00ZNice quiet location and a comfortable bed. Easy walk to lots of restaurants and bars.
Stephen2016-03-09T00:00:00ZPaul communicated well with me and the room was a clean and bright loft conversion. I think there was a little trouble with the plumbing, but Paul was so attentive, I am sure he is on top of that. All in all I had a delightful stay.
Lucy2014-11-13T00:00:00ZVery welcoming hosts, friendly, warm and welcoming. First experience of airbnb and very positive it was too. Great room, clean and tidy in a nice house. Neighbourhood is pleasant and very close to the town.
West Bridgford, NG2 6BA, สหราชอาณาจักร
West Bridgford, England NG2 7PY, สหราชอาณาจักร
Would highly recommend staying here - a big and light room in a great flat. Location excellent and everything you could need provided. Top class.
Will2016-07-06T00:00:00ZAnna was very nice and helpful. Extremely clean flat exactly as described. Good location a short bus ride from Nottingham station. Well situated for West Bridgford high street.
Linda2016-09-28T00:00:00ZAnna is a lovely host, great at keeping in contact before our trip and really friendly, welcoming and helpful when we got there. We found the bed very comfortable and slept very well and Anna let us help ourselves to breakfast in the morning. Overall a fab place to stay.
Matt and Hannah2015-10-23T00:00:00ZI had a great stay with Anna, she was a great host, really friendly and welcoming. The flat was in a perfect location and it was clean and tidy. I'd definitely recommend staying here.
Will2016-05-30T00:00:00ZAnna made us feel very welcome, the flat was great in a perfect position close to Trent Bridge and the centre of town. Anna also left beer, milk and bread. Her home was exactly how it was advertised.
Peter2015-08-11T00:00:00ZAnna is a remarquable host who makes you fell at home and at ease. My first night in Nottingham was very pleasant thanks to her hospitality and the area is very peaceful.
West Bridgford, Nottinghamshire NG2 7PS, สหราชอาณาจักร


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subset: commoncrawl


text:
I was at CVS a while back to pick up a prescription. But of course, being me, I can't just walk to the pharmacy and back out again...I have to make a little side trip through the cosmetics section. As usual I didn't have a lot of extra money to spend but I had $5.50 worth of extra care bucks (or whatever the heck they are called) burning a hole in my wallet. After a disappointing walk-through I walked by an endcap that make my jaw drop. They actually had a whole endcap completely devoted to the Wet 'n Wild Fantasy Makers collection! And it was completely stocked! And it had a big yellow sign that said it was BOGO! How did I get so lucky? I don't know but I quickly took advantage of the situation. This is what I ended up grabbing:
Between my extra care bucks and the BOGO deal I got all of these lovelies for $1.50! I know right? I was pretty damn excited I tell ya. And here are the 3 glosses swatched in sunlight. I tried to capture the holo goodness in these babies because it is super pretty.
L-R: Snow White, Queen Of Hearts, Wicked Witch
And here they are on me. Looking at these pics I'm thinking maybe I should have smiled a bit or something? I'm not used to doing makeup pics yet so bear with me...
Snow White is something I would definitely wear layered over something else to give it a bit of sparkle.I'm still a bit gun shy when it comes to red lippies so Queen of Hearts is a perfect way to ease my way into wearing it. I love the holo sparkle to it! (It kinda looks like I have a smear of it above my lip but I don't remember seeing it at the time...huh...)
Wicked Witch will probably only get love on Halloween. As I work in the medical field, it is definitely not work-appropriate and I am definitely gun shy about wearing black lippies. I really love it though!
These are definitely awesome glosses for the price ($1.99). I don't like glosses that are too slippery or too thick....I like a consistency somewhere in the middle and these fit the bill. I actually think I'll get quite a bit of wear out of Queen of Hearts and I will bust out Wicked Witch on Halloween for sure. I have an idea for a cool eye look to go with it. If it turns out the way I envision it, I'll show you guys some pics.
'Til We Meet Again...
Rebecca :)


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Möchtest du als deine Standardwährung festlegen oder nur für diese Sitzung behalten?
Clean rooms. We faced a patio area but it was quiet at night. Nice to bring our dog with us- the pet rooms had tiled floors The breakfast buffet is ok but not great food. The dog run was gross- nobody cleaned up after their dog. Not an attractive setting but good for a stop over for a night
The hotel location was good for where I need to go on this trip to Deadwood. I would of liked a fridge in my room. When traveling on business I like to have that for my water, juices and food items that need refrigeration. It was not a deal breaker though. I would book a room again here.
Roxy the housekeeper and Dan , not sure of his title . Really added a nice touch to the stay. Very personable and full of laughs. These two definitely added to my stay
My fiance and I loved it the room was nice the accomadations were nice we accidentallyreserved wrong night but the customer service person we spoke with fixed and we enjoyed so much we stayed another night Nothing loved all of it
All the outlets everybody charges there phones and electronics.
Hidden fees. Poor quality bed and pillows.
clean comfortable rooms, free soda and coffee, nice gaming selection, the girls climbing on the fabric into the ceiling was entertaining, not too sexy, but a lot of work for them. They were breathing heavy when they got done with their flips. Nice check in and out, good gamers club, free drinks while gambling, free premium drinks when in the dollar games. All was good
Clean and comfortable. No activities for children, except watchingtelevisoin.
Scenic with a small creek running through the property and the machines payed reasonably
A\C in rooms was nice, once it cooled down. Gambling facility is nice. Black Jack table not available all the time. Add said there was a pool at this hotel, when there is not one! Also, a lot of. Construction both at hotel and in area. A\C. Non exist in dining area. Nothing for kids to do at this site.
breakfast casino and room hot tub far away from room no elevators to 2nd floor
The breakfast was good, the back deck was nice after a long day of sight seeing. No stairs to top floor.
Staff and Restaurant are excellent. Beds were hard.
Both people we dealt with seemed a bit abrupt when we dealt with them. It didn't seem very friendly and was not the best impression when first checking in to the hotel.
Very friendly staff. more like a home than a hotel room. Exquisite for the price. Loved the old style elevator. There was nothing about the hotel i didnt like
It was beautifully decorated! Staff was very nice There was no fridge in the room
The staff goes out of their way to help you with anything you need. The owners were fantastic people. Therr was not anything we did not like.
The staff No
Friendly hosts, beautiful old house.
Staff and breakfasts were excellent
It is a very nice bed and breakfast that has a lot of character. There was an excellent breakfast in the morning. I'd prefer to have my own room, but knew ahead of time that we were sharing a bathroom.
Helpful, courteous, friendly personnel. Great breakfast. Convenient location. none
This guest did not leave comments about this hotel.
This guest did not leave comments about this hotel.
Loved that I could open my window and see and hear the creek behind the hotel. Nice updated rooms and good caramel rolls in am. Parking was tight on Saturday night.
The room was spacious and it was pretty quite. There was a deck in back by the river. The bathroom was not very clean. We had a cooler with pop go missing from our room. The evening staff said they would check, and we never heard a thing. The day time staff the next morning was better. Breakfast was not very good, except for the rolls.
The price and location. The walls were very thin so you could hear your neighbors easily. The noise from the road came through the windows easily.
hot tub and clean rooms small rooms
Very clean, staff was friendly. Pool very close to the casino. No
Location & priced well for the location & facility. Had a handicapped room & shower head would NOT stay fixed in position that you put it in & hard to get it to stay where you needed it to stay. Otherwise, all was fine.
This guest did not leave comments about this hotel.
This guest did not leave comments about this hotel.
This guest did not leave comments about this hotel.
We loved our nice roomy accomadations, the cleanliness of the room, the staff was very friendly and courteous and helpful with our questions we asked. Also we loved the shuttle van and the trolley rides to our fun places we went to in the area-no hassles! No, Loved everything about it!ðŸ'😃
Loved it.
Close to downtown. Clean rooms. Some signs of date built in pool area and arcade. Cool trail across the street. Not downtown. No. Fair price. Clean rooms. Rigjt on the main road. Not downtown. A few blocks up the hill toward Lead. Check out lead Homestake mine.
The room was very comfortable and clean. The staff at the front desk were very friendly and helpful as was the hotel casino staff. I was not sure what to expect with a 2 star rating. Will stay there again if we return.
It was a cozy and clean room No
This guest did not leave comments about this hotel.
This guest did not leave comments about this hotel.
Very nice clean rooms, great helpful staff The location was somewhat lacking. Other than that very nice
This guest did not leave comments about this hotel.
Clean & accommodating rooms No complaints!
Shuttle service to anywhere in deadwood Room had unusual Oder
This guest did not leave comments about this hotel.
Deadwood is a great city to have a fun time in.\nThe Holiday Inn Express is a nice hotel, but the parking is horrible.\nRooms were very clean, the staff was quite helpful.\nCasino is right next door ~
No compensation given when we had to defer check-in for several hours due to a water leak. The delay caused us great inconvenience in our birthday weekend plans. Elevator didn't work after we checked in and no one available to help with luggage to 4th floor room. We tried to talk to management, but not available on site. Followed with a letter to express our concern about the staff's handling of our discomfort, but no reply. Wrote to IHG Guest Relations who forwarded letter to local management...still no response from hotel. All of the guests this particular weekend were upset as it was a holiday for which many had made plans far in advance. A small gesture of making sure that our bags were in our rooms and a drink in the bar while we waited would have made a huge difference in the attitudes of guests. Our complaint is not with the rooms, but with the staff. The water break was unfortunate and I'm sure had the staff flustered, but when you are in the SERVICE business, guests should not...
This hotel is in the heart of downtown Deadwood, making it possible to walk to all the casino's and shops. The room was adequately sized, and my husband and I loved that there were pillows labeled 'soft' and 'firm' so we didn't spend time beating them to find the right one to sleep on! The bathroom was a thing of beauty. Granite counter tops, a large tub.shower, fluffy towels, and great products provided! The free morning breakfast was also good, with eggs, rolls, coffee and cold cereal. Plenty to start the day.
The hotel/rooms were very clean and the staff profes- sional, polite and efficient. Step out the front door and you're on Deadwood's infamous Main Street. Unless something off-the-wall happens, you should have a great time.
After traveling to Deadwood many years in the Winter, this is the preferred hotel, convenience, breakfast!!! staff, whatever we need.
What a lovely surprise to find this little gem in downtown Deadwood. Luxurious rooms, quiet, great/accomodating staff
The Hotel is right on Main Street - Newly remodeled - Nice Rooms Spotless - Great Breakfest - Staff very Nice - Spent 3 Days in June 2011 - Ran the Deadwood Marathon - and will choose it again next Year !
Just be careful when you stumble out the door, you might see Wild Bill as you are in the heart of Deadwood
This hotel was very nice. We had a great stay. The rooms were very clean and comfortable. There were plenty of pillows, towels etc. The staff was pleasant and helpful. We would definately stay again.
The central location was perfect for our family. We have a newborn and was very convenient to run to if needed. The hotel is a little more expensive than other Deadwood hotels, but worth it for us.
friendly front desk personnel, perfect location to main street, comfy beds you don't want to get out of!
Staff was awesome Loved everything
Room was comfortable enough. Place reminded you of a college dorm. Cleanliness OK. Spotty wi-fi service. Very limited food service. Poor casino--slots only, small and deserted. Few TV channels, mostly sports. Bottom line, for about the same price or just a little more one of the casino resorts such as The Lodge is a better idea.


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8245 Meadowlark Drive,Carlisle OH, 45005
Last Updated: 3/28/2017 12:09 PM
Description for
8245 Meadowlark Drive
Carlisle, OH 45005
Check out this beautiful home. Features a screened in florida room, two car garage and ready to move in. Immediate occupancy available.
Listing Information
Well Water
Public Sewer
School Information
Room Information
Interior Features
Central Air
Electric Heating,
Forced Air
Exterior / Lot Features
2 Garage Spaces,
Attached Garage, Driveway
Financial Considerations
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Active Market Trends
Source:MLS DATA
Median list price$159,900Days on market103Properties for sale13Sold Market Trends
Source:MLS DATA
Median sold price$158,950Sales to list price9,566.0%Properties sold26Median sq ft price$86 Arrows indicate 90 day trend
Listing Price History for 8245 Meadowlark Drive
Carlisle Real EstateCarlisle is located in Ohio. Carlisle, Ohio 45005 has a population of 5,259. The median household income in Carlisle, Ohio 45005 is $52,043. The median household income for the surrounding county is $73,177 compared to the national median of $53,482. The median age of people living in Carlisle 45005 is 40.8 years.
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text:
Brooks Helps Thunder Grow
Now, in his fifth season with the Oklahoma City Thunder as the team's head coach, Brooks has created a stable environment for his young, emerging Thunder squad. Being an NBA Champion with the Houston Rockets in 1994 and creating a ten-year NBA career for himself, Brooks approaches his job as a coach from a position of understanding what it's like to be a player.
That's why it is his philosophy to always defer credit for the Thunder's rise into a contender over the past five seasons. While All-Stars like Kevin Durant and Russell Westbrook were elite talents coming out of college, Coach Brooks has helped mold them and the others on the roster into a cohesive unit.
"He's the most humble guy I've ever been around," Durant said. "He's a great role model, first off. He may have a great defensive scheme or a great offensive strategy and he gives us all the credit."
At the INTEGRIS Health Thunder Development Center, it often seems that praise flies around the room deflecting off those who receive it in order to heap it on teammates, coaches or others within the organization. Every member of the Thunder operation understands that without one another's assistance and teamwork, the unit would not be as strong.
As a result, for all of the times Brooks gushes about his players' hard work and dedication, his players turn around and remind those outside the facility's walls that Brooks and his coaching staff have been instrumental in the process. To grow under-20 players like Durant, Westbrook and Serge Ibaka into high-level contributors who fit together within a team concept in just a matter of years is a remarkable accomplishment for Brooks and company.
"He's putting us in great positions," Durant said. "He and the coaching staff do such a great job with us in game time and practice time and development as well."
Not only has Brooks helped the Thunder players work on their individual skills and develop them as players, he's also made sure to help them understand team concepts at a higher level as the seasons go by. Primarily Brooks is focused on assisting his unit problem-solve on the defensive end of the floor.
With the highest scoring offense in the NBA, Brooks knows he's developed his players' offensive talents. Thanks to the assistance of veteran, defensive-minded players like Kendrick Perkins and Nick Collison, Brooks has been able to effectively emphasize that end of the floor more and more each practice, each game and each season. With his players' focus devoted to the defensive end, Brooks allows his players freedom on offense.
"If you work on your game and you work on your jump-shot, he never complains about offense," Perkins said. "I guess that's why we're one of the league leaders. He's been great at out of time outs, drawing up plays and defensive schemes. One thing about Scotty that you have to love is that he gives you the freedom to go out and play on the offensive end."
Sometimes NBA games are perceived in terms of a single player going against another player, primarily on the offensive end, but Brooks understands the chess match involved on both ends of the floor, among all five players. This season the 47-year old head coach has shown off some crafty in-bounds plays, unique offensive sets and smart defensive strategies that have helped put Thunder players in the best position possible to succeed.
"It's hard," Perkins said. "(A coach has) to watch the match-ups; you have to put guys in the right position on the court to know what is going to work. Everything is done for a reason."
It's easy to understand how young players like Durant, Westbrook and Ibaka, along with rookies Perry Jones and Jeremy Lamb are still developing with each month of the season. It's more nuanced, however, to contemplate the ways in which Brooks himself is still growing and improving as a head coach.
Brooks helps make sure no one in the Thunder organization sees themselves as a finished product, but rather a gradually maturing person finding ways to help the team. No one falls outside of that philosophy of perpetual improvement and dedication to process, particularly not the head coach himself.
"He's learning along with us," Durant said. "He is a young coach but he doesn't use that as an excuse… He's one of the great young coaches in this league, and I'm looking forward to growing with him."


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Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome
© Lamontagne et al; licensee BioMed Central Ltd. 2010
Received: 18 November 2009
Accepted: 12 May 2010
Published: 12 May 2010
Brucellosis is a major bacterial zoonosis affecting domestic livestock and wild mammals, as well as humans around the globe. While conducting proteomics studies to better understand Brucella abortus virulence, we consolidated the proteomic data collected and compared it to publically available genomic data.
The proteomic data was compiled from several independent comparative studies of Brucella abortus that used either outer membrane blebs, cytosols, or whole bacteria grown in media, as well as intracellular bacteria recovered at different times following macrophage infection. We identified a total of 621 bacterial proteins that were differentially expressed in a condition-specific manner. For 305 of these proteins we provide the first experimental evidence of their expression. Using a custom-built protein sequence database, we uncovered 7 annotation errors. We provide experimental evidence of expression of 5 genes that were originally annotated as non-expressed pseudogenes, as well as start site annotation errors for 2 other genes.
An essential element for ensuring correct functional studies is the correspondence between reported genome sequences and subsequent proteomics studies. In this study, we have used proteomics evidence to confirm expression of multiple proteins previously considered to be putative, as well as correct annotation errors in the genome of Brucella abortus strain 2308.
Brucella species bacteria are gram negative alpha proteobacteria superbly adapted for survival in intracellular environments. They infect a wide range of mammals, including essentially all economically important domestic mammals, many wild species, and humans. Brucellosis is the largest bacterial zoonosis in the world [1–3]. In humans, untreated brucellosis is a long lasting disease characterized by recurrent fever episodes and clinical manifestations that include spondylitis, severe headaches, joint or abdominal pain, endocarditis, and meningoencephalitis. In severe non-treated cases brucellosis can cause death [1–3].
Seven terrestrial Brucella species have been defined: Brucella melitensis, Brucella abortus, Brucella suis, Brucella ovis, Brucella canis, Brucella neotomae and Brucella microti which infect goats, cattle, pigs, sheep, dogs, desert wood rats and common voles, respectively [1, 4]. Two Brucella species infecting marine mammals such as dolphins, whales, seals, sea lions and walrus have also been defined as Brucella ceti and Brucella pinnipedialis [5–7]. With the exception of B. suis biovar 3, the Brucella genome is encoded on two chromosomes, containing in total approximately 3,500 genes. Genome sequences from 32 different Brucella strains, representing all species, have been published either as complete genomes (10 strains) or as draft assemblies in NCBI (22 strains) [8–14]. The raw genome sequencing data of 78 other strains is also available in the Sequence Read Archive of NCBI. The genome sequences were very highly homologous, although regions of unique genetic material were also observed. It is possible that these regions are involved in establishing the distinct host preferences and biological behavior of the different Brucella species sequenced to date [15].
Unlike other pathogenic bacteria, Brucella virulence does not appear to be the result of relatively few virulence genes that can be transferred horizontally via plasmids, phages, or assembled in pathogenicity islands. Brucella also lack typical virulence factors such as exotoxins, flagella, capsules, and type III secretion systems. Rather, the pathogen's virulence appears to be an integrated aspect of its physiology. Therefore, to better understand Brucella virulence, we will need to better understand the Brucella proteome, including how it changes during the different stages of the intracellular and extracellular Brucella lifecycles, and how it interacts with host proteins and processes. Indeed, we have previously demonstrated that Brucella bacteria are capable of extensive, reversible, remodeling of their cell envelopes [16]. Furthermore, during the establishment of an intracellular infection, Brucella bacteria also appear able to carry out extensive, and reversible, modifications to their biosynthetic pathways and respiration in order to adapt to the changing microenvironments encountered in infected host cells [17]. This suggests that the Brucella proteome is considerably more dynamic than previously suspected, and that in depth proteomic analysis of the pathogen, as well as integration of these data with the available genomic information, will result in novel mechanistic and possibly therapeutic insights.
In this work we have generated a synthesis of the proteomic datasets we produced from multiple independent comparisons of Brucella strains either grown in media or retrieved from infected host cells. Some of this data is currently publicly available [[16, 17];http://proteomicsresource.org/Default.aspx] with the remainder becoming available as part of this work. These studies were originally designed to identify experimental condition-specific differences in the Brucella proteome. We compiled the experimental evidence for any Brucella protein detected and compared the proteomic data to the available genomic data. We provide the first direct experimental evidence for the expression of 305 Brucella proteins, but also identified experimental evidence for the expression of five genes previously annotated as pseudogenes, and of start site errors in two other genes.
Results and Discussion
First experimental evidence of the expression of 305 proteins in B. abortus 2308
B. abortus 2308 proteins for which the expression was demonstrated for the first time
Cytoplasm | |||||||
BAB1_0002 | DnaN | BAB1_0855 | GRX family | BAB1_1449 | UDP-N- | BAB1_2149 | PepS |
BAB1_0022 | Unknown | BAB1_0856 | BolA-related | acetylmuramate | BAB1_2168 | RpsO; S15 | |
BAB1_0023 | AroA | BAB1_0857 | FGAM synthase II | L-alanine ligase | BAB1_2173 | FabB | |
BAB1_0035 | KdsB | BAB1_0861 | PurS | BAB1_1508 | CarB | BAB2_0083 | Eda2 |
BAB1_0063 | Unknown | BAB1_0864 | HpcH/HpaI | BAB1_1512 | CspA | BAB2_0090 | GCN5-related |
BAB1_0071 | ArgG | BAB1_0874 | AcpP | BAB1_1523 | GreA | N-acetyltransferase | |
BAB1_0100 | Putative AsnC family | BAB1_0880 | HAD-like | BAB1_1528 | SseA-1 | BAB2_0109 | Gnd |
BAB1_0107 | Trs-ABC (P-loop) | BAB1_0886 | NN:DBI PRT | BAB1_1538 | OmpR | BAB2_0160 | Unknown |
BAB1_0118 | Unknown | BAB1_0896 | ArgS | BAB1_1547 | PepQ | BAB2_0162 | L-carnitine |
BAB1_0122 | GyrB | BAB1_0898 | NagZ | BAB1_1549 | PrsA | dehydratase | |
BAB1_0139 | NifU | BAB1_0918 | GatB/Yqey | BAB1_1553 | YchF | BAB2_0177 | YafB |
BAB1_0159 | S30EA | BAB1_0924 | AccC | BAB1_1613 | Unknown | BAB2_0186 | Fumarate hydratase |
BAB1_0160 | PtsN-like | BAB1_0933 | PCRF 2 | BAB1_1645 | DhaK-1 | BAB2_0187 | Unknown |
BAB1_0191 | GABAtrnsam | BAB1_0943 | TyrS | BAB1_1646 | DhaK-2 | BAB2_0191 | HAD-like, |
BAB1_0204 | AdhP | BAB1_0949 | SufC | BAB1_1655 | GabD | subfamily IIA | |
BAB1_0215 | ThiE | BAB1_0955 | DeaD | BAB1_1669 | PAS domain | BAB2_0198 | Pseudouridine |
BAB1_0216 | ThiG | BAB1_0960 | Trs heavy metal | BAB1_1671 | TcaR | synthase | |
BAB1_0242 | ManR | BAB1_1014 | MetG | BAB1_1687 | Dut | BAB2_0216 | 3-hydroxybutyryl-CoA |
BAB1_0285 | HisD | BAB1_1030 | Gor | BAB1_1695 | PurA | dehydrogenase | |
BAB1_0317 | Trs arginine/ornithine | BAB1_1037 | Mandelate racemase; | BAB1_1702 | Phosphoglucosamine | BAB2_0246 | P47K |
BAB1_0331 | ArgD | muconate lactonizing | mutase | BAB2_0293 | Gal | ||
BAB1_0344 | Pip | BAB1_1043 | Unknown | BAB1_1719 | ThiE | BAB2_0295 | DgoK |
BAB1_0353 | Unknown | BAB1_1050 | FolB | BAB1_1722 | Efp | BAB2_0296 | KdgA |
dehydrogenase | BAB1_1077 | Ach1p | BAB1_1751 | Unknown | BAB2_0335 | NADH:flavin oxidore- | |
BAB1_0416 | DUF85 | BAB1_1096 | NifU-like | BAB1_1761 | PyK | ductase/NADH oxidase | |
BAB1_0429 | Polyprenyl synthetase | BAB1_1098 | PRA-CH | BAB1_1778 | FdxA | BAB2_0337 | RocF |
BAB1_0446 | DnaJ | BAB1_1121 | DNA gyrase subunit A | BAB1_1781 | Unknown | BAB2_0343 | Trx-2 |
BAB1_0447 | FabI-1 | BAB1_1130 | ClpA/B | BAB1_1804 | MarR family | BAB2_0358 | Dcp |
BAB1_0482 | FabD | BAB1_1132 | ClpP | BAB1_1810 | AtpH | BAB2_0361 | TypA |
BAB1_0484 | AcpP | BAB1_1156 | KdsA | BAB1_1813 | Transaldolase | BAB2_0365 | FbaA |
BAB1_0489 | Guanylate kinase | BAB1_1157 | PyrG | BAB1_1815 | LeuS | BAB2_0366 | RpiB/LacA/LacB |
BAB1_0510 | ThrC | BAB1_1161 | TpiA | BAB1_1819 | ACAT | BAB2_0367 | TIM 2 |
BAB1_0525 | PpdK | BAB1_1164 | TrpC | BAB1_1824 | PurH | BAB2_0370 | EryC |
BAB1_0532 | Transthyretin | BAB1_1169 | GltX | BAB1_1837 | CynT | BAB2_0448 | Unknown |
BAB1_0540 | Formyl transferase, | BAB1_1170 | GltA | BAB1_1840 | MmsA | BAB2_0457 | FolD |
N-terminal | BAB1_1174 | FabZ | BAB1_1872 | PrfA | BAB2_0459 | Pgl | |
BAB1_0544 | DegT/DnrJ/EryC1/StrS | BAB1_1187 | Endoribonuclease | BAB1_1874 | LysC | BAB2_0460 | Zwf |
BAB1_0561 | Man-6-P isomerase | L-PSP | BAB1_1879 | GrxC | BAB2_0483 | ShuT | |
type II | BAB1_1188 | GDPD | BAB1_1887 | HemC | BAB2_0513 | GcvT | |
BAB1_0570 | XylA | BAB1_1205 | ElaB-domain | BAB1_1895 | FtsK-gamma | BAB2_0518 | PutA |
BAB1_0587 | Unknown | BAB1_1212 | BhbA | BAB1_1918 | LpdA-2 | BAB2_0566 | AldA |
BAB1_0588 | ATP/GTP-binding | BAB1_1213 | Unknown; conserved | BAB1_1926 | SucC | BAB2_0568 | Unknown |
BAB1_0641 | Alanine aminopep- | BAB1_1223 | AlaS | BAB1_1936 | GloB | BAB2_0572 | IlvE |
tidase: Neutral zinc | BAB1_1224 | RecA | BAB1_1946 | SecA | BAB2_0620 | Unknown | |
metallopeptidase, | BAB1_1233 | RpsM; S13 | BAB1_1970 | FadB | BAB2_0642 | Acyl-CoA | |
zinc-binding region | BAB1_1234 | Adk | BAB1_1971 | EtfA | dehydrogenase | ||
BAB1_0666 | DapA | BAB1_1241 | RpsH; S8 | BAB1_1988 | HisC | BAB2_0644 | Metal-dependent |
BAB1_0671 | RpoZ | BAB1_1242 | RpsN; S14 | BAB1_1993 | Ppa | hydrolase | |
BAB1_0688 | PyrC-1 | BAB1_1244 | RplX; L24 | BAB1_2006 | RegA | BAB2_0645 | GatC |
BAB1_0697 | CysS | BAB1_1245 | RplN; L14 | BAB1_2016 | RpmB; L28 | BAB2_0646 | GatA |
BAB1_0718 | MoaD | BAB1_1248 | RplP; L16 | BAB1_2023 | ClpA/clpB | BAB2_0851 | GuaB |
BAB1_0740 | Unknown | BAB1_1249 | RpsC; S3 | BAB1_2059 | ParB | BAB2_0961 | DapA |
BAB1_0775 | AspS | BAB1_1256 | RpsJ; S10 | BAB1_2080 | HslU | BAB2_0976 | AldB |
BAB1_0780 | HemB | BAB1_1266 | RplJ; L10 | BAB1_2081 | HslV | BAB2_0988 | ArgB |
BAB1_0787 | GlyA | BAB1_1280 | Unknown | BAB1_2087 | HisE | BAB2_0990 | Unknown |
BAB1_0789 | RibD | BAB1_1286 | GloA | BAB1_2096 | PTS system IIA | BAB2_0991 | DapD |
BAB1_0790 | RibE | BAB1_1294 | Aminotransferase | subunit | BAB2_0993 | DapE | |
BAB1_0813 | CysD | BAB1_1297 | Unknown | BAB1_2109 | AccD | BAB2_1009 | MgsA |
BAB1_0817 | Unknown; conserved | BAB1_1376 | UreA | BAB1_2133 | Unknown | BAB2_1012 | DapB |
BAB1_0826 | NuoE | BAB1_1408 | IlvB | BAB1_2134 | SMP-30 | BAB2_1013 | Gpm |
BAB1_0842 | ProS | BAB1_2135 | Glutathione synthetase | ||||
Inner membrane | |||||||
BAB1_0400 | Unknown | BAB1_1283 | DUF192 | BAB2_0261 | RecA | BAB2_0877 | Binding-protein- |
BAB1_0425 | NhaA | BAB1_1703 | FtsH | BAB2_0709 | FtsK-alpha | dependent transport | |
BAB1_0542 | WbkC | BAB1_1712 | MotA; TolQ; ExbB | BAB2_0728 | CydA | system inner | |
membrane component | |||||||
Periplasm | |||||||
BAB1_0010 | Trs-ABC oligopeptide | BAB1_1118 | PpiB-1 | BAB2_0427 | Trs-ABC spermidine/putrescine | BAB2_0697 | Unknown; conserved |
BAB1_0155 | OstA-like | BAB1_1362 | LacI | BAB2_0812 | Trs-ABC oligopeptide | ||
BAB1_0404 | Unknown | BAB1_1413 | DegP | BAB2_0451 | Trs-ABC oligopeptide | AppA family | |
BAB1_0444 | PdxH | BAB1_1890 | YciI-like protein | AppA family | BAB2_0879 | Trs-ABC spermidine/putrescine | |
BAB1_0739 | ETC complex I | BAB1_1919 | Unknown | BAB2_0593 | Trs-ABC amino acid | ||
BAB1_0776 | Unknown | BAB1_1981 | TlpA | BAB2_0611 | Trs-ABC amino acid | BAB2_0880 | Unknown |
BAB1_0881 | Trs-ABC amino acid | BAB2_0374 | Unknown | BAB2_0664 | Trs-ABC peptide | BAB2_1109 | XylF |
BAB1_1117 | PpiB-2 | ||||||
Outer membrane | |||||||
BAB1_0659 | Omp2a | BAB1_0707 | OstA | BAB1_0963 | TolC | ||
Unknown localization | |||||||
BAB1_0030 | Unknown | BAB1_0991 | Unknown | BAB1_1543 | DUF526 | BAB1_2123 | RpmI; L35 |
BAB1_0170 | GrpE | BAB1_1070 | WrbA | BAB1_1559 | FbcF | BAB1_2176 | YaeC/NLPA lipoprotein |
BAB1_0389 | CcoP | BAB1_1113 | Unknown; conserved | BAB1_1641 | Unknown | BAB1_2186 | RpsT; S20 |
BAB1_0413 | AtpB | BAB1_1152 | PdhA | BAB1_1647 | FabG domain | BAB2_0207 | Unknown |
BAB1_0418 | Unknown | BAB1_1230 | RplQ; L17 | BAB1_1693 | bZIP | BAB2_0243 | YedY |
BAB1_0420 | Unknown | BAB1_1232 | RpsK; S11 | BAB1_1728 | RpmE; L31 | BAB2_0269 | RpsU; S21 |
BAB1_0453 | Unknown | BAB1_1240 | PplF; L6 | BAB1_1749 | Unknown | BAB2_0351 | OsmC-like protein |
BAB1_0479 | RpsR, S18 | BAB1_1260 | RpsL; S12 | BAB1_1768 | Unknown | BAB2_0356 | Unknown |
BAB1_0627 | Unknown | BAB1_1270 | SecE | BAB1_1784 | DUF336 | BAB2_0677 | Unknown |
BAB1_0650 | Unknown | BAB1_1341 | Unknown | BAB1_1814 | Unknown | BAB2_0726 | YbgT |
BAB1_0810 | RpsI; S9 | BAB1_1384 | Cibk | BAB1_1858 | RplU; L21 | BAB2_0869 | HlyD |
BAB1_0830 | NDH-1 subunit I | BAB1_1514 | AspC | BAB1_1984 | LysA | BAB2_1002 | NqoB |
Correction of five pseudogene annotations
Correction of two start site annotations errors
Another type of annotation error identified in our studies was the erroneous assignment of gene translation start sites. For 2 proteins of B. abortus 2308, we report the expression of manually validated peptides corresponding to the sequence found upstream of their currently annotated start sites (Figure 2). The peptide sequence "MNIHEYQAK" was first found to match the cytoplasmic B. melitensis succinyl-CoA synthetase subunit beta protein (BMEI0138) and then assigned manually to BAB1_1926. Sequence comparison with other Brucella species and strains shows that the B. abortus 2308 protein start site is not shared with any of the subject sequences (Figure 2A). In fact, all homologues of this protein in other Brucella strains or species share the same start site, which is found 22 amino acids upstream of the B. abortus 2308 site. Moreover, a ribosome binding site can clearly be mapped to position -8 of the proposed new translation start site. We therefore believe this new start site to be accurate.
The second peptide, "TDLLPIMK", was found to match the cytoplasmic B. melitensis keto-hydroxyglutarate-aldolase (BMEII0009) and then assigned to BAB2_0083 in B. abortus 2308. This peptide overlaps the region upstream to the currently annotated translation start site and the first three amino acids based on the annotated translation start site (Figure 2B). Alignment of the current B. abortus 2308 protein sequence with its counterparts in other Brucella strains and species indicates that the 2308 protein sequence is falsely truncated. Other start sites lead to proteins having N-terminals longer by 11, 26 or 44 amino acids. Although we cannot clearly indicate the actual start site of BAB1_1926 or BAB2_0083, we can confirm that their N-terminals are longer than currently annotated. Based on the homology of the B. abortus 2308 genome being highest with that of other B. abortus strains, one can speculate that the start sites would be identical to those mapped in these strains.
Since genes that are part of an operon are usually co-transcribed, it is possible that these genes might also be co-translated [32]. Considering all proteins identified by our studies, we were able to almost fully reconstitute one of the two ribosomal RNA operons, with all but BAB1_1237 found. Additionally, the previously mentioned BAB1_1645 and BAB1_1646 genes are predicted to be part of an operon containing 6 genes, BAB1_1645 to BAB1_1650 http://www.microbesonline.org/operons/gnc359391.html. Four of these proteins were detected in our studies, although only BAB1_1645, -46 and -48 were found in the same experimental condition.
Mass spectrometry has proven to be a valuable tool to identify and correct genomic annotation errors in the study of microorganisms [33–37]. We performed a proteomics analysis of B. abortus 2308 proteins expressed upon extracellular and intracellular growth conditions to validate existing gene predictions at the protein level, to acquire useful information on B. abortus 2308 expressed proteins and to identify and correct inaccurately annotated ORFs. We were able to confirm the expression of over 300 previously unreported proteins and five pseudogenes, and corrected two wrongly assigned translation start sites. Taken together, these findings further demonstrate that computational genomic annotation errors can be corrected using proteomics. This will lead to improved databases and thus better protein identification and functional annotation.
Brucella abortus protein preparation for mass spectrometry analysis
Four types of B. abortus 2308 samples were prepared: outer membranes, cytosols, intracellular bacteria isolated from infected RAW264.7 macrophages and extracellular bacteria from overnight cultures. Outer membrane samples were prepared and processed for mass spectrometry analysis as previously described [16]. Cytoplasmic fractions were prepared as described previously [38]. Briefly, bacteria grown in tryptic soy broth (Difco) in 2-liter flasks on an orbital shaker and harvested by centrifugation in sealed cups at 7,000 × g for 20 min. The thick slurry of bacteria were suspended in 10 mM phosphate-buffered saline (pH 7.2) was passed twice through a French press (Pressure Cell 40 K, Aminco; SLM Instruments Inc., Urbana, Ill.) at an internal pressure of 35,000 lb/in2. The homogenate was digested with 50 mg of DNase II type V and RNase A per ml (Sigma) for 18 h at 37°C and fractionated by ultracentrifugation. The cell envelopes in the bottom of the tube removed and the cytoplasmic fractions in the supernatant, filtered, lyophilized and characterized as described previously [39]. Intracellular bacteria were isolated from RAW264.7 macrophages 3, 20 and 44 hours post-infection as previously described [17]. Proteins were extracted from intracellular and extracellular bacteria using the same method and digested for mass spectrometry as previously described [17].
Liquid Chromatography - Mass Spectrometry (LC-MS)
Peptide digests were analyzed by liquid chromatography coupled to mass spectrometry (LC-MS) as described [40]. Briefly, the samples were injected onto a reversed-phase column (Jupiter C18, Phenomenex, Torrance, CA) for HPLC separation. For LC-MS survey scans, the mass spectra were acquired over 400-1600 Da at a rate of 1 spectrum/second. Peptide sequencing was achieved by targeted and shotgun LC-MS/MS. For MS/MS scans, the mass range was 50-2000 Da, and each spectrum was acquired in 2 seconds. For LC-MS/MS, the duty cycle was one survey scan followed by one product ion scan (MS/MS).
Protein identification
Protein identification was done by submitting LC-MS/MS spectra to Mascot software (MatrixScience, Boston, MA) and searching against custom protein databases (see below). The parameters used for the Mascot search and protein homology clustering were previously detailed [16]. No multidimensional fingerprinting method was used. Annotation for each protein was performed using ExPASy Proteomics tools http://us.expasy.org/tools/#proteome, Kegg GenomeNet Database Service http://www.genome.jp/ and literature mining of orthologous genes and proteins.
Protein databases
The databases were composed of protein sequences obtained from the National Center for Biotechnology Information (NCBI) protein database (for B. abortus 2308, NC_007618 and NC_007624; for B. melitensis 16 M, NC_003317 and NC_003318; for Mus musculus, all protein sequences contained under taxonomy ID 10090) and of B. abortus 2308 "pseudoproteins" corresponding to the custom translation of pseudogenes. Genomic regions corresponding to the 316 entries annotated as pseudogenes in NCBI were directly translated and added to the database. Additionally, the ORF Finder tool from NCBI was used to determine other possible protein sequences corresponding to the pseudogenes. The ORF search was done by including 0 to 200 bp upstream or downstream from these regions. All resulting ORFs spanning the entire pseudogene sequence were kept. Ribosome binding sites were mapped when possible according to the sequence described in reference [41]. A total of 471 translated protein sequences were added to the NCBI databases.
Validation of mass spectrometry results
Sequences assigned to MS/MS spectra of peptides, which were mapped to pseudogenes or to genomic regions annotated as untranslated regions, were manually validated. For proteins identified by a single peptide, manual validation of the spectra was performed for peptide sequences having a Mascot score below 45.
Prediction of protein localization
The localization of newly demonstrated proteins was predicted using PSORTb version 2.0.4 http://www.psort.org/psortb/index.html, CELLO version 2.5 http://cello.life.nctu.edu.tw/ and PSLpred http://www.imtech.res.in/raghava/pslpred/index.html. For a localization to be assigned, a minimum of 2 of the 3 predictions had to match.
This work was funded by the NIAID/NIH contract HHSN266200400056C.
Authors' Affiliations
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text:
Archdale in Guilford County, North Carolina — The American South (South Atlantic)
Mustering out of Confederate Army
Location. 35° 54.857′ N, 79° 58.291′ W. Marker is in Archdale, North Carolina, in Guilford County. Marker is on North Main Street (U.S. 311) near Liberty Road (North Carolina Highway 62). Click for map. Marker is in this post office area: High Point NC 27263, United States of America.
Other nearby markers. At least 8 other markers are within 4 miles of this marker, measured as the crow flies. Springfield Friends Meeting (approx. 0.9 miles away); Trinity Cemetery (approx. 1.2 miles away); Model Farm (approx. 1.7 miles away); Trinity College (approx. 1.8 miles away); a different marker also named Trinity College (approx. 1.8 miles away); The Plank Road Foreman (approx. 3.5 miles away); Revolutionary War Patriots (approx. 3.5 miles away); High Point (approx. 3.5 miles away).
Categories. • War, US Civil •
Credits. This page originally submitted on , by Stanley and Terrie Howard of Greer, South Carolina. This page has been viewed 1,008 times since then and 17 times this year. Photos: 1. submitted on , by Bill Coughlin of North Arlington, New Jersey. 2. submitted on , by Stanley and Terrie Howard of Greer, South Carolina. 3. submitted on , by Bill Coughlin of North Arlington, New Jersey. • Craig Swain was the editor who published this page. This page was last revised on June 16, 2016.


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#1 New York Times bestselling author Dean Koontz delivers a thrilling novel of suspense and adventure, as the lives of strangers converge around a mystery unfolding high in the Colorado mountains—and the balance of the world begins to tilt….
In the stillness of a golden September afternoon, deep in the wilderness of the Rockies, a solitary craftsman, Grady Adams, and his magnificent Irish wolfhound Merlin step from shadow into light…and into an encounter with enchantment. That night, through the trees, under the moon, a pair of singular animals will watch Grady's isolated home, waiting to make their approach.
A few miles away, Camillia Rivers, a local veterinarian, begins to unravel the threads of a puzzle that will bring all the forces of a government in peril to her door.
At a nearby farm, long-estranged identical twins come together to begin a descent into darkness…In Las Vegas, a specialist in chaos theory probes the boundaries of the unknowable…On a Seattle golf course, two men make matter-of-fact arrangements for murder…Along a highway by the sea, a vagrant scarred by the past begins a trek toward his destiny…
In a novel that is at once wholly of our time and timeless, fearless and funny, Dean Koontz takes readers into the moment between one turn of the world and the next, across the border between knowing and mystery. It is a journey that will leave all who take it Breathless .
"synopsis" may belong to another edition of this title.
Dean Koontz, the author of many #1 New York Times bestsellers, lives with his wife, Gerda, and the enduring spirit of their golden retriever, Trixie, in southern California.
Excerpt. © Reprinted by permission. All rights reserved.:Chapter One
A moment before the encounter, a strange expectancy overcame Grady Adams, a sense that he and Merlin were not alone.
In good weather and bad, Grady and the dog walked the woods and the meadows for two hours every day. In the wilderness, he was relieved of the need to think about anything other than the smells and sounds and textures of nature, the play of light and shadow, the way ahead, and the way home.
Generations of deer had made this path through the forest, toward a meadow of grass and fragrant clover.
Merlin led the way, seemingly indifferent to the spoor of the deer and the possibility of glimpsing the white flags of their tails ahead of him. He was a three-year-old, 160-pound Irish wolfhound, thirty-six inches tall, measured from his withers to the ground, his head higher on a muscular neck.
The dog's rough coat was a mix of ash-gray and darker charcoal. In the evergreen shadows, he sometimes seemed to be a shadow, too, but one not tethered to its source.
As the path approached the edge of the woods, the sunshine beyond the trees suddenly looked peculiar. The light turned coppery, as if the world, bewitched, had revolved toward sunset hours ahead of schedule. With a sequined glimmer, afternoon sun shimmered down upon the meadow.
As Merlin passed between two pines, stepping onto open ground, a vague apprehension—a presentiment of pending contact—gripped Grady. He hesitated in the woodland gloom before following the dog.
In the open, the light was neither coppery nor glimmering, as it had appeared from among the trees. The pale-blue arch of sky and emerald arms of forest embraced the meadow.
No breeze stirred the golden grass, and the late-September day was as hushed as any vault deep in the earth.
Merlin stood motionless, head raised, alert, eyes fixed intently on something distant in the meadow. Wolfhounds were thought to have the keenest eyesight of all breeds of dogs.
The back of Grady's neck still prickled. The perception lingered that something uncanny would occur. He wondered if this feeling arose from his own intuition or might be inspired by the dog's tension.
Standing beside the immense hound, seeking what his companion saw, Grady studied the field, which gently descended southward to another vastness of forest. Nothing moved . . . until something did.
A white form, supple and swift. And then another.
The pair of animals appeared to be ascending the meadow less by intention than by the consequence of their play. They chased each other, tumbled, rolled, sprang up, and challenged each other again in a frolicsome spirit that could not be mistaken for fighting.
Where the grass stood tallest, they almost vanished, but often they were fully visible. Because they remained in motion, however, their precise nature was difficult to define.
Their fur was uniformly white. They weighed perhaps fifty or sixty pounds, as large as midsize dogs. But they were not dogs.
They appeared to be as limber and quick as cats. But they were not cats.
Although he'd lived in these mountains until he was seventeen, though he had returned four years previously, at the age of thirty-two, Grady had never before seen creatures like these.
Powerful body tense, Merlin watched the playful pair.
Having raised him from a pup, having spent the past three years with little company other than the dog, Grady knew him well enough to read his emotions and his state of mind. Merlin was intrigued but puzzled, and his puzzlement made him wary.
The unknown animals were large enough to be formidable predators if they had claws and sharp teeth. At this distance, Grady could not determine if they were carnivores, omnivores, or herbivores, though the last classification was the least likely.
Merlin seemed to be unafraid. Because of their great size, strength, and history as hunters, Irish wolfhounds were all but fearless. Although their disposition was peaceable and their nature affectionate, they had been known to stand off packs of wolves and to kill an attacking pit bull with one bite and a violent shake.
When the white-furred creatures were sixty or seventy feet away, they became aware of being watched. They halted, raised their heads.
The birdless sky, the shadowy woods, and the meadow remained under a spell of eerie silence. Grady had the peculiar notion that if he moved, his boots would press no sound from the ground under him, and that if he shouted, he would have no voice.
To get a better view of man and dog, one of the white creatures rose, sitting on its haunches in the manner of a squirrel.
Grady wished he had brought binoculars. As far as he could tell, the animal had no projecting muzzle; its black nose lay in nearly the same plane as its eyes. Distance foiled further analysis.
Abruptly the day exhaled. A breeze sighed in the trees behind Grady.
In the meadow, the risen creature dropped back onto all fours, and the pair raced away, seeming to glide more than sprint. Their sleek white forms soon vanished into the golden grass.
The dog looked up inquiringly. Grady said, "Let's have a look."
Where the mysterious animals had gamboled, the grass was bent and tramped. No bare earth meant no paw prints.
Merlin led his master along the trail until the meadow ended where the woods resumed.
A cloud shadow passed over them and seemed to be drawn into the forest as a draft draws smoke.
Gazing through the serried trees into the gloom, Grady felt watched. If the white-furred pair could climb, they might be in a high green bower, cloaked in pine boughs and not easily spotted.
Although he was a hunter by breed and blood, with a Sher?lockian sense of smell that could follow the thinnest thread of unraveled scent, Merlin showed no interest in further pursuit.
They followed the tree line west, then northwest, along the curve of meadow, circling toward home as the quickening air whispered through the grass. They returned to the north woods.
Around them, the soft chorus of nature arose once more: birds in song, the drone of insects, the arthritic creak of heavy evergreen boughs troubled by their own weight.
Although the unnatural hush had relented, Grady remained disturbed by a sense of the uncanny. Every time he glanced back, no stalker was apparent, yet he felt that he and Merlin were not alone.
On a long rise, they came to a stream that slithered down well-worn shelves of rock. Where the trees parted, the sun revealed silver scales on the water, which was elsewhere dark and smooth.
With other sounds masked by the hiss and gurgle of the stream, Grady wanted more than ever to look back. He resisted the paranoid urge until his companion halted, turned, and stared downhill.
He did not have to crouch in order to rest one hand on the wolfhound's back. Merlin's body was tight with tension.
The big dog scanned the woods. His high-set ears tipped forward slightly. His nostrils flared and quivered.
Merlin held that posture for so long, Grady began to think the dog was not so much searching for anything as he was warning away a pursuer. Yet he did not growl.
When at last the wolfhound set off toward home once more, he moved faster than before, and Grady Adams matched the dog's pace.
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