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Invited talks
- IT University; Department of IT Management and Leadership. Copenhagen, Denmark. (Online lecture)
- University of Tilburg; Department of Organization Studies. Tilburg, Netherlands.
- Managing Innovation and Entrepreneurship in Family Firms (California Management Review Special Issue Workshop). Politecnico di Milano. Milan, Italy.
- Purdue University; Research Center for Open Digital Innovation. West-Lafayette, IN, United States. (Online lecture)
- IASP European Division Workshop ("The most efficient way to organize an Open Innovation Marketplace"), Poznan Science and Technology Park, Poznan, Poland.
- Roskilde University; Department of Communication, Business and Information Technologies. Roskilde, Denmark.
- IT University; Department of IT Management and Leadership. Copenhagen, Denmark.
- Chalmers University of Technology; Department of Technology Management and Economics. Gothenburg, Sweden.
- Innovation Forum 2013. Tata Institute of Social Sciences (TISS). Mumbai, India. (Video lecture)
- Scuola Superiore Sant'Anna; Institute of Management. Pisa, Italy. (Seminar)
- University of Trento; School of Social Sciences. Trento, Italy. (Seminar)
- Aarhus University; Department of Business Administration. Aarhus, Denmark. (Seminar)
- Open Innovation Speaker Series. University of California, Berkeley, USA. (Seminar)
- Open Innovation: New Insights and Evidence. Imperial College London, London, UK. (Conference)
- ESC Rennes School of Business; Center of Technology and Innovation Management, Rennes, France. (Seminar)
- University of Manchester; Manchester Business School. Manchester, UK. (Seminar)
- University of Amsterdam; Amsterdam Business School. Amsterdam, Netherlands. (Seminar)
- Aalborg University; Department of Business and Management, Aalborg, Denmark. (Seminar)
- University of Southern Denmark; Strategic Organization Design. Odense, Denmark. (Seminar)
- Ludwig-Maximilians-Universität München; Institute for Strategy, Technology and Organization. Munich, Germany. (Seminar)
- University of Groningen; Innovation Management and Strategy. Groningen, Netherlands. (Seminar)
- Open Source, Innovation, and New Organizational Forms. Institutions and Political Economy Group (IPEG), University of the Witwatersrand (Wits), Johannesburg, South Africa. (Conference)
- User Innovation and Communities: Taking Stock and Looking Ahead. Aarhus, Denmark. (Workshop)
- International Conference on Invention, Innovation and Commercialization with Special Emphasis on Technology Users' Innovation (TUI). Christchurch, New Zealand. (Conference)
- Copenhagen Business School; Center for Strategic Management and Globalization. Copenhagen, Denmark. (Seminar)
- University of Southern Denmark; Integrative Innovation Management. Odense, Denmark. (Seminar)
- National University of Singapore; Division of Engineering & Technology Management. Singapore. (Seminar)
- University of Twente; Operations, Organization and Human Resources. Enschede, Netherlands. (Seminar)
- University of Southern Denmark; Mads Clausen Institute for Product Innovation. Sønderborg, Denmark. (Seminar)
- Danish Technical University; Center for Technology, Economics and Management. Copenhagen, Denmark. (Seminar)
- University of Tilburg; Organisation & Strategy. Tilburg, Netherlands. (Seminar)
- Eindhoven University of Technology; Organisation Science and Marketing. Eindhoven, Netherlands. (Seminar)
- Erasmus University; Rotterdam School of Management. Rotterdam, Netherlands. (Seminar)
- University of Groningen; Innovation Management and Strategy. Groningen, Netherlands. (Seminar)
- User Innovation Indicators Workshop. NESTA, London, UK. (Workshop)


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Coffs Harbour Campus, SCU
SCU's Coffs Harbor campus boasts of its friendly learning environment equipped with modern facilities such as its purpose-built nursing laboratory, complete with simulated wards. The National Marine Science Center can also be found here as well as the Coffs Harbor Technology Park.
The Coffs Harbor campus offers courses on Arts, Social Sciences, Psychology, Business as well as Information Technology and many others.
Student Accommodation
Student residence is available a few minutes away from the academic precinct. SCU Village apartments include four individual bedrooms, a shared bathroom, a full kitchen and access to phones and the Internet.
For more information on student housing, click here or here.
About Coffs Harbor
Nestled between mountains and beaches, Coffs Harbor possesses Australia's most livable climate. The city subsists mostly on farming, tourism and manufacturing. Apart from SCU's campus, Coffs also has a number of radio stations, a private hospital and major shopping centers.
Famous for its banana plantations, Coffs Harbor is also home to the Big Banana, a part of Australia's Big Things attraction.
Coffs Harbor experiences humid sub-tropical climate with seasonal rainfalls.

subset: commoncrawl


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Diners, Drive-Ins and Dives All Family, All the Time Highlights
Three Angels Fried Chicken 02:34
Videos In This Playlist
Opal's Thai Hot Wings 03:19
Guy's introduced to Thai-style hot wings at Opal Thai Food.
Opal Thai Food 02:31
Guy's flavor jets get turned on in a big way at Opal Thai Food.
Anthonino's Tavern 03:32
In St. Louis, Guy tries real Italian-Greek cuisine at Anthonino's Tavern.
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The mechanisms underlying asthmatic airway epithelial injury are not clear. 12/15-lipoxygenase (an ortholog of human 15-LOX-1), which is induced by IL-13, is associated with mitochondrial degradation in reticulocytes at physiological conditions. In this study, we showed that 12/15-LOX expressed in nonepithelial cells caused epithelial injury in asthma pathogenesis. While 12/15-LOX overexpression or IL-13 administration to naïve mice showed airway epithelial injury, 12/15-LOX knockout/knockdown in allergic mice reduced airway epithelial injury. The constitutive expression of 15-LOX-1 in bronchial epithelia of normal human lungs further indicated that epithelial 15-LOX-1 may not cause epithelial injury. 12/15-LOX expression is increased in various inflammatory cells in allergic mice. Though non-epithelial cells such as macrophages or fibroblasts released 12/15-LOX metabolites upon IL-13 induction, bronchial epithelia didn't release. Further 12-S-HETE, arachidonic acid metabolite of 12/15-LOX leads to epithelial injury. These findings suggested 12/15-LOX expressed in non-epithelial cells such as macrophages and fibroblasts leads to bronchial epithelial injury.
Current estimates have shown a steep increase in the incidence of various respiratory diseases such as asthma, chronic obstructive pulmonary disease, etc1. So there is a necessity to explore the novel signaling pathways or novel pathogenetic mechanisms for existing candidate molecules for a better understanding of the disease and effective management. Asthma, a chronic airway inflammatory disease, is characterized by airway hyperresponsiveness to nonspecific stimuli, airway obstruction, bronchovascular inflammation, airway eosinophilia, and airway remodeling changes. These asthma features are predominantly due to imbalance in Th1/Th2 response2,3 and this imbalance could lead to increase the proinflammatory mediators and oxidative stress in the airway which affects structural cells as a bystander effect. Among injured structural cells, airway epithelium is having crucial importance as it is the primary barrier against exogenous and endogenous stress with its innate mechanisms. Recent literature demonstrated that airway epithelia can determine the immune status of lung by recruiting particular type of immune cells through its armamentarium of cytokines such as interlekin-25 (IL-25), IL-33 and thymic stromal lymphopoietin4,5,6. Due to these reasons current respiratory researchers have given a central role for airway epithelia in pathogenesis of lung diseases7,8,9. In this context, we and others have demonstrated the importance of mitochondrial function in relation to asthmatic epithelial injury10,11,12. Restoration of mitochondrial function of alveolar epithelia can heal the lung injury indicates the enormous potency of mitochondria in maintaining epithelial health13. Thus, it is equally important to explore the mechanisms for the airway epithelial injury.
IL-13 is the dominant cytokine in asthma pathogenesis14. Its role in airway inflammation and airway remodeling including goblet cell metaplasia is well studied15. Though IL-13 is known to cause intestinal epithelial apoptosis16, however it's effect in causing airway epithelial apoptosis in in vivo is not clear. Since IL-13 induced goblet cell metaplasia needs epithelial survival before conversion of Clara cell to goblet cells15, the effect of IL-13 on epithelial apoptosis might have not been considered. 12/15-lipoxygenase (12/15-LOX) has been found to be consistently upregulated in IL-13 transgenic mice, ovalbumin (OVA) induced mice and also in asthmatic bronchial epithelia17. On the one hand, 12/15-LOX degrades mitochondria of reticulocytes in the process of RBC maturation by peroxidizing the mitochondrial membranes18 and on the other hand its deficiency alleviated asthma features19. Though our earlier studies20,21 indicated that 12/15-LOX is linked to mitochondrial dysfunction by using vitamin E and esculetin, the direct evidence of 12/15-LOX to cause airway epithelial injury and cellular involvement are still lacking. With this context, we hypothesized that 12/15-LOX may cause airway injury in asthma pathogenesis. In this study, we have shown that 12/15-LOX present in non-epithelial cells such as macrophages leads to bronchial epithelial injury.
To determine the effect of 12/15-LOX on airway injury, control or 12/15-LOX overexpression plasmid was administered intravenously or intranasally to healthy Balb/c mouse as shown in Figure 1A. Intravenous administration showed a significant expression of 12/15-LOX in lung though intranasal administration also showed a sufficient expression (Fig. 1B); so intravenous route was selected for further studies. 12/15-LOX overexpressed lungs also showed an increase in the activity of 12/15-LOX with an increase in the major metabolites such as 12-(S)-Hydroxyeicosatetraenoic acid (12-S-HETE) and 13-(S)-hydroxyoctadecadienoic acid (13-S-HODE) (Fig. 1C, D). Interestingly, 12/15-LOX overexpression plasmid administered mice had shown a spontaneous airway hyperresponsiveness (AHR), recruitment of inflammatory cells in the bronchovascular regions, increased inflammation score, moderate degree of subepithelial fibrosis and scarce goblet cell metaplasia compared to control plasmid administered mice even without allergen induction (Fig. 1E–I).
Next we wanted to determine the effect of 12/15-LOX overexpression (OE) on airway injury and mitochondrial dysfunction. 12/15-LOX OE mice had shown a decrease in complex IV activity and mitochondrial membrane potential in lung mitochondria and increase in cytochrome C in lung cytosol (CYTO) (Fig. 2A–C). Further, 12/15-LOX OE mice had shown an increase in caspase 3 activity, and increase in the number of apoptotic bronchial epithelia (Fig. 2D–F). Also there were mitochondrial structural changes such as loss of cristae in the bronchial epithelia of 12/15-LOX OE mice (Fig. 2G).
To confirm the effect of 12/15-LOX on airway epithelial injury, OVA induced Balb/c mice were administered scrambled small interfering RNA (siRNA) or 12/15-LOX siRNA (Fig. S1A). Ovalbumin– sensitized and –challenged mice showed the increased 12/15-LOX expression with increase in its linoleic acid metabolite, 13-S-HODE (Fig. S1B, C). Ovalbumin– sensitization and –challenge led to the recruitment of inflammatory cells into the airway, goblet cell metaplasia and AHR (Figs. S1D, S2A, B). Asthmatic mice treated with scrambled siRNA had the features of mitochondrial dysfunction with a reduction in the activities of mitochondrial complexes I and IV, decrease in the mitochondrial membrane potential in lung mitochondria and increase in cytochrome C in lung cytosol (Fig. 3A–D). However, 12/15-LOX siRNA treated asthmatic mice had shown the increased activities of mitochondrial complexes I and IV along with an increase in mitochondrial membrane potential in lung mitochondria and decrease in cytochrome C in lung cytosol (Fig. 3A–D). While scrambled siRNA treated asthmatic mice had shown an increase in caspase 3 activity in lung cytosol, increase in apoptosis of bronchial epithelia and mitochondrial structural changes such as loss of cristae and swelling, 12/15-LOX siRNA administered mice had shown the reduction in caspase 3 activity, reduction in airway epithelial injury and restoration of mitochondrial ultrastructural changes in bronchial epithelia, along with the attenuation of asthma features (Figs. 3E–G, S2A–H).
To confirm these findings further, we have used 12/15-LOX knockout (KO) mice (C57BL/6). Sham controls of 12/15-LOX KO mice had shown a normal mitochondrial function with no increase in cytochrome C in lung cytosol along with normal architecture of lung compared to wild type Sham controls though there was a reduction in the number of lymphocytes in bronchoalveolar lavage (BAL) fluid (Figs. 3H, S2I–L). Ovalbumin– sensitization and –challenge causes increase in the levels of cytochrome C in lung cytosol along with airway inflammation, airway eosinophilia, goblet cell metaplasia, subepithelial fibrosis, and increase in airway hyperresponsiveness in wild type C57BL/6 mice (Fig. 3H and Fig. S2I–L). However, 12/15-LOX KO mice upon ovalbumin- sensitization and -challenge had shown the reduction in cytochrome C in lung cytosol with reduction in asthma features (Fig. 3H and Fig. S2I–L).
Though it is known that there are various cellular sources of 12/15-LOX in human, 12/15-LOX expressing cells in mice are not clear. To determine the cellular involvement underlying bronchial epithelial injury, we have performed immunohistochemistry (IHC) for 12/15-LOX in lung sections of asthmatic mice and normal mice. Surprisingly, we didn't find the 12/15-LOX expression in the bronchial epithelia of normal and asthmatic mice (Fig. 4A). However, there were significant expressions in various inflammatory cells present in bronchovascular regions in asthmatic mice. Higher magnifications revealed that these cells may be granulocytes such as neutrophils and eosinophils, macrophages and monocytes in asthmatic mouse. To confirm the type of inflammatory cells that express 12/15-LOX, co-immunostaining experiments were performed in single cell suspensions of lungs. Various inflammatory cells such as eosinophils (Siglec F), macrophages (CD 11b, F4/80), dendritic cells (CD 11c), T lymphocytes (CD 3), and granulocytes (Gr-1) (Fig. 4B) had shown the increased expression of 12-LOX indicate the possible crosstalk between inflammatory cells and bronchial epithelia to cause epithelial injury.
To determine the human relevance of observed findings in mouse, first we have performed IHC in lungs of patients with asthma and healthy individuals. More interestingly, even normal human bronchial epithelia had shown a drastic expression of 15-LOX-1 though lungs of asthmatic patients had shown the expression both in the epithelia and in inflammatory cells such as macrophages (Fig. 5A, B).
The constitutive expression of 15-LOX-1 in normal human airway indicated that epithelial 12/15-LOX may not lead to epithelial injury. Since we found an increased activeness of 12/15-LOX with increase in the metabolites in 12/15-LOX overexpressed mice which showed the bronchial epithelial injury (Fig. 1C, D), we suspect that 12/15-LOX metabolites may be crucial in causing airway epithelial injury. To determine this, we measured the levels of 12-S-HETE in sera, sputum supernatants and BAL fluid supernatants of atopic asthmatics and compared with non-atopic unrelated control individuals. There was a marked increase of 12-S-HETE in biological secretions in atopic asthmatics whereas 12-S-HETE was found to be in negligible quantity in normal individuals (Fig. 6A). The similar trend was observed in the levels of 13-S-HODE (data not shown). This indicates that human bronchial epithelia may not be responsible for the 12/15-LOX metabolites which were measured in the airway.
To determine this, bronchial epithelia were induced with IL-13 and crucial metabolite, 13-S-HODE, was determined as 13-S-HODE is produced in larger concentrations at the time of mitochondrial degradation in reticulocytes18. IL-13 induction of human bronchial epithelial cells led to a significant increase in 13-S-HODE levels in the cytosol but not in the culture supernatant (Fig. 6B). In addition, the induction of human bronchial epithelial cells with IL-13 combination with either IL-4 or TNF-α did not increase the 13-S-HODE levels in the culture supernatant (Fig. 6C). However, IL-13 induced the release of 13-S-HODE from macrophages or fibroblasts (Fig. 6D). Further, culture supernatants of IL-13 induced macrophage reduced mitochondrial membrane potential of bronchial epithelia which was comparable to carbonyl cyanide m-chlorophenylhydrazone (cccp) treated bronchial epithelia (Fig. 6E). The mean fluorescence intensity of red aggregates (cells have healthy mitochondria) was 105.5 ± 0.5 in bronchial epithelia that were incubated with uninduced macrophage culture supernatant (mean ± sem) whereas it was 76.3 ± 0.8 in bronchial epithelia that were incubated with IL-13 induced macrophage culture supernatant. In addition, culture supernatants of IL-13 induced macrophage increase the release of cytochrome C in cytosol in bronchial epithelia (Fig. 6F). Further, exogenous 12-S-HETE, a predominant metabolite of 12/15-LOX, also released the cytochrome C in a dose dependent manner (Fig. 6F).
As we found IL-13 induced macrophage lead to epithelial injury (Fig. 6E, F) and IL-13 alone didn't lead to epithelial injury (data not shown), we wanted to determine the effect of IL-13 on epithelial injury in in vivo model. While IL-13 converts bronchial epithelia into mucus hyper-secreting goblet or pro-fibrotic phenotype22, the effect of IL-13 on bronchial epithelia is not well studied. For this, we administered recombinant IL-13 (rIL-13) intranasally to Balb/c mice that were treated with scrambled small interfering RNA (siRNA) or 12/15-LOX siRNA (Fig. S3A). IL-13 administration led to increase in the 12/15-LOX expression, and in the levels of 12-S-HETE and 13-S-HODE in lung cytosol (Fig. S3B–D). Expectedly, IL-13 administration led to asthmatic features such as AHR, airway inflammation, goblet cell metaplasia, and subepithelial fibrosis (Fig. S3E, and Fig. S4A–C). Importantly, IL-13 administration leads to the features of mitochondrial dysfunction as there was a decrease in the activities of complexes I and IV and mitochondrial membrane potential in lung mitochondria and increase in the levels of cytochrome C in lung cytosol (Fig. 7A–D). In addition, we found a drastic increase in apoptotic positive bronchial epithelia in IL-13 administered mice (Fig. 7E, F). Further, IL-13 administered mice had shown the mitochondrial structural changes in bronchial epithelia (Fig. 7G). However, all these IL-13 mediated features were attenuated with 12/15-LOX knockdown (Fig. 7A–G, Fig. S3B–E, and Fig. S4A–C).
To confirm these findings, rIL-13 was administered to C57BL/6 mice those were wild type or genetically deleted for 12/15-LOX. IL-13 administered wild type mice had shown the features of mitochondrial dysfunction with decrease in the activity of complex I and increase in the levels of cytochrome C in lung cytosol (Fig. 7H–I). IL-13 induced mitochondrial dysfunction was associated with airway inflammation, goblet cell metaplasia and subepithelial fibrosis and increase in levels of 13-S-HODE (Fig. S4D–G). However, IL-13 administered 12/15-LOX KO mice had shown the restoration of the mitochondrial function along with the reduction in asthma features (Fig. 7H–I and Fig. S4D–G). These results demonstrated that 12/15-LOX is essential in IL-13 mediated epithelial injury and asthma features.
Current human studies in asthma revealed that mechanisms for different types of asthma converge towards epithelia and suggested that epithelium could be the great contributor in asthma23. In this context, mitochondria seem to be crucial in airway epithelial function and pathogenesis of lung diseases10,11,12,13. Thus, understanding the mechanisms for mitochondrial dysfunction and airway epithelial injury are equally important for effective therapeutic strategies.
Though it is known that the deficiency of 12/15-LOX attenuates asthma features, its role in airway injury is not yet investigated. 12/15-LOX has profound biological importance since they directly oxygenates lipid-protein assemblies such as biomembranes even without prior phospholipase A2 action and it is shown to be increased in inflammatory conditions and chronic diseases such as atherosclerosis, diabetes and Alzheimer's disease24,25. Importantly, IL-4 and IL-13 induces 12/15-LOX expression in various cell types including bronchial epithelia26,27,28. 12/15-LOX act as an amplifier of oxidative stress as it produces fatty acyl hydroperoxides which further reacts with active redox molecules of mitochondria to generate reactive oxygen species that cause mitochondrial depolarization by forming permeability-transition pore29. Though this phenomenon has been explained at the physiological conditions whether abnormal increase of 12/15-LOX would have any effect on mitochondrial function and thus lung health and asthma pathogenesis was not known. In this study, we demonstrated that 12/15-LOX overexpression in naïve mice was associated with mitochondrial dysfunction and bronchial epithelial injury and on the other hand 12/15-LOX knockout/knockdown in allergic mice reduced airway epithelial injury, indicated that 12/15-LOX may cause airway epithelial injury. Importantly, 12/15-LOX overexpression also leads to spontaneous airway hyperresponsiveness even without allergen induction.
Though IL-13 is crucial in causing most of the asthma features its exact role in bronchial epithelial injury was not known. Recent success with anti–interleukin-13 therapy in asthmatic patients who had higher epithelial derived periostin levels30 further emphasized the importance of IL-13 on epithelial function. As expected, IL-13 didn't cause epithelial apoptosis in our in vitro studies (data not shown), whereas it leads to significant apoptosis of bronchial epithelia in mice. These indicate the possible involvement of nonepithelial sources in causing epithelial injury. To understand this, we performed IHC for 12/15-LOX in both mouse and human asthmatic lungs. Surprisingly, there was a constitutive expression of 15-LOX-1 in bronchial epithelia of normal human beings whereas those were not expressed in murine bronchial epithelia even under asthmatic conditions. This indicated that 12/15-LOX present in other cell types could be responsible for the observed epithelial injury in 12/15-LOX overexpressed mice. Evidently, there were significant expressions in various inflammatory cells such as macrophages in asthmatic mouse and human lungs. These findings indicate that 12/15-LOX expression in epithelia may not cause mitochondrial dysfunction and airway epithelial injury and also indicated the two possible modes for epithelial injury: a) 12/15-LOX activeness and b) crosstalk between inflammatory cells and bronchial epithelia through 12/15-LOX.
Evidently, both 12/15-LOX overexpressed naïve mice and IL-13 administered naïve mice which showed mitochondrial dysfunction and airway epithelial injury also showed a significant increase in the levels of metabolites indicate the activeness of 12/15-LOX may be important for causing airway epithelial injury. Further these metabolites were absent in 12/15-LOX deficient mice which showed the reduction in airway epithelial injury. Thus, 12/15-LOX activity, production of 12/15-LOX metabolites, seems to be crucial to cause epithelial injury. To determine the activeness of 12/15-LOX in human asthmatics, we measured 12-S-HETE in airway secretions which are close proximity to airway epithelia. Our findings showed that 12-S-HETE was found to be increased in sera, sputum and BAL fluid supernatants of atopic asthmatic patients whereas normal individuals had shown a negligible quantity of 12-S-HETE. The similar trend was observed for the levels of 13-S-HODE in our recent study31. These findings indicate the 12/15-LOX activeness is crucial in causing mitochondrial dysfunction.
We also found that though 13-S-HODE was found to be increased inside the cells there was no increase in the culture supernatants indicates bronchial epithelia couldn't release these metabolites. To support these findings another study demonstrated that 15-S-HETE is also not released from bronchial epithelia and intracellular 15-S-HETE leads to Muc5AC induction32. However, this was not true in case of other cell types such as macrophages or fibroblasts as they release 13-S-HODE. In addition, 12/15-LOX metabolites rich culture supernatants of macrophages or exogenous 12-S-HETE lead to increase the release of cytochrome C confirms that crosstalk between inflammatory cells and bronchial epithelia to cause epithelial injury. To support our findings, 12-S-HETE has been shown to increase intramitochondrial calcium overload and apoptosis in cardiac myocytes33. Also 13-hydroxylinoleic acid, one of the products of 12/15-LOX, had shown to increase airway hyperresponsivess in guinea pigs34. In this context, extracellular 13-S-HODE causes an increase in intramitochondrial calcium overload and apoptosis in bronchial epithelia31. These indicate that the possibly non-epithelial cells may release 12/15-LOX metabolites such as 12-S-HETE into the airway secretions and these metabolites could cause airway epithelial injury due to immediate vicinity of airway secretions to airway epithelia by activating receptors present on the epithelial surface35,36,37 or diffusing in the bronchial epithelia. This needs further investigations.
The reasons and mechanisms for this differential spatial regulation are not clear right now. Collectively these indicate the existence of a delicate balance between different fates of bronchial epithelia such as goblet cell metaplasia and apoptosis. The fate of epithelia may be decided by severity of allergic inflammation and inducibility of 12/15-LOX in different cell types with IL-13. Further, 12/15-LOX has a number of other products, some of which are anti-inflammatory, such as resolvin, lipoxin, and protectin38,39. Thus, further studies are required to dissect the role of individual metabolites on airway injury.
In conclusion, our findings revealed a novel role in 12/15-LOX in asthma pathogenesis and also previously unknown function for IL-13 in causing 12/15-LOX mediated mitochondrial dysfunction and bronchial epithelial injury. These findings would be helpful in understanding epithelial injury and formulating therapeutic strategies targeting epithelial injury.
Balb/c mice (Male, 6–8 weeks, obtained from Central Drug Research Institute, Lucknow, India or National Institute of Nutrition, Hyderabad, India and maintained in IGIB, Delhi) and wild-type or 12/15-LOX knockout C57BL/6 mice (obtained from Jackson Laboratories, USA and maintained in Johns Hopkins University, Baltimore, MD, USA) were used. The respective institutional animal ethics committees approved all animal experiments. There were three different murine models and various groups as shown in Table 1.
In OVA model, mice were immunized and challenged40,41. Briefly, every mouse was immunized by three intraperitoneal (i.p.) administrations of 50 μg OVA (Grade V chicken egg ovalbumin, Sigma, St. Louis, MO, USA) in 4 mg alum or 4 mg alum alone on days 0, 7 and 14. Immunized mice were then challenged after a week with 3% OVA aerosol or PBS 30 min a day for 7 consecutive days with a nebulizer. Mice under isoflurane anesthesia were administered 100 μg scrambled or 12/15-LOX siRNA (Sigma, USA) intranasally. In 12/15-LOX overexpression model, pCMV6 or pCMV6-12/15-LOX was complexed with commercially available polyethylenimine (PEI) based transfection reagent (in vivo-jetPEI, Polyplus-transfection, Illkirch, France) in 5% glucose solution and injected into the tail vein (50 μg) or instilled into the nostrils (25 μg) in isoflurane anesthetized mice. Results from our earlier study42 indicated that intravenously injected plasmids complexed with in vivo-jetPEI or in vivo-jetPEI effectively entered into the lungs. In IL-13 model, we instilled either 30 μl (3 μg) of carrier-free recombinant murine IL-13 (R&D Systems) in 1% Bovine serum albumin (BSA) or 1% BSA alone into the nasal openings in each isoflurane-anesthetized mouse.
AHR in the form of airway resistance in anesthetized mice was determined with flexiVent system (Scireq) that uses the computer-controlled mouse ventilator to measure respiratory mechanics40,41,42. The results were expressed in the percentage baseline airway resistance with increasing concentrations of methacholine (Mch) assuming PBS aerosol induced baseline values as 100%. After the AHR measurement, bronchoalveolar lavage was performed by instilling 2 ml PBS into the tracheostomized airway and recovered BAL fluids (approximately) were processed to get cell pellet that was stained with Leishman stain to determine differential cell count as described earlier10,40,41,42. After BAL fluid collection, lungs were removed and fixed with 10% formalin. Fixed lungs were further processed and embedded with paraffin. 5-μm paraffin embedded lung sections were stained with hematoxylin and eosin, periodic acid-Schiff, and Masson Trichrome stainings for assessing the inflammation, mucus hypersecretion and subepithelial fibrosis, respectively. Stained sections were viewed and photomicrographs were taken by microscope attached with camera (Eclipse 90i). The inflammation scoring was performed by experimentally blind experts to find the perivascular, peribronchial and total airway inflammation as described earlier10,40,41,42.
To determine the apoptosis in the lungs, 5-μm paraffin embedded lung sections were incubated with biotinylated nucleotides which end-labels the fragmented DNA of apoptotic cells in the presence terminal deoxynucleotidyl transferase and horseradish peroxidase-labeled streptavidin and detected with H2O2, DAB system (Promega, USA). As a result apoptotic nuclei stained in brown color. Among various cell types positive for apoptosis, bronchial epithelia were the predominant cell types. So the number of apoptosis positive bronchial epithelia was determined with experimentally blind investigators to determine the apoptotic index which was expressed in the percentage positive bronchial epithelia31,43. To determine the ultrastructural changes in bronchial epithelia, the combined in situ perfusion and immersion fixation was performed as described previously10. The perfused and fixed lungs were dissected with dissection microscope (Olympus, SZX model) to find the first generation bronchi. Dissected bronchi were further processed to make blocks which were further stained and visualized with transmission electron microscope10.
Mitochondrial and cytosolic fractions were prepared from the freshly harvested lungs (Sigma, St. Louis, MO, USA)10. Briefly, lungs were homogenized in isotonic HEPES buffer containing fat free albumin and low speed centrifugation (600 g for 6 min) was performed to remove cellular debris and the resultant supernatants were centrifuged at high speed centrifugation (11000 g for 12 min). The resultant cell pellets were processed again with both low speed and high speed centrifugation to get mitochondrial pellets and cytosolic fractions. Both the fractions were estimated for protein quantities with BCA assay (Sigma, St. Louis, MO, USA). Mitochondrial membrane potential (Sigma, St. Louis, MO, USA), activities of mitochondrial complexes I (Mitosciences, USA) and IV (Sigma, St. Louis, MO, USA) in isolated lung mitochondria were estimated according to manufacturer's instructions10,31.
Lung cytosolic proteins were used for Western blot analysis of 12/15-LOX21 (Santa Cruz, CA, USA). Briefly, lung cytosolic proteins were separated with SDS-PAGE electrophoresis, transferred to PVDF membranes those were blocked and incubated with 12/15-LOX (Santa Cruz, USA) or α-tubulin (Sigma, St. Louis, MO, USA) antibody followed by respective HRP conjugated secondary antibodies (Sigma, St. Louis, MO, USA) and the membranes were developed with DAB-H2O2 system (Sigma, St. Louis, MO, USA). Immunohistochemical analysis31 for 12/15-LOX was performed with 12/15-LOX and respective HRP conjugated secondary antibody (Sigma, St. Louis, MO, USA). Briefly, 5-μm paraffin embedded lung sections were rehydrated, endogenous peroxidase was eliminated, incubated with 12/15-LOX antibody followed by HRP conjugated secondary antibody and developed with DAB system (Sigma, St. Louis, MO, USA). Isotype control (Jackson Immunoresearch Laboratories) or omission of primary antibody was used as a negative control.
Cytochrome C, 13-(S)-hydroxyoctadecadienoic acid (13-S-HODE), 12-(S)-Hydroxyeicosatetraenoic acid (12-S-HETE) in serum, cytosol of lung, bronchial epithelial cell lysate or culture supernatants were estimated according to manufacturer instructions. Caspase 3 activity in lung cytosols was estimated as described earlier21.
We have utilized sera of atopic asthmatics and healthy controls recruited for our earlier studies44,45. The Review Board at the CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi, India, approved the studies, and we also obtained written informed consent from the human participants. Few participants were recruited for the collection of sputum and BAL fluid with ethical approval from the Review Board at the All India Institute of Medical Sciences, New Delhi, India. The sputum induction and BAL collection were performed as described46. Human lung samples were obtained from Biochain (Hayward, CA, USA) and the All India Institute of Medical Sciences. Ethical Review Board at IGIB approved to use of these samples.
Human bronchial epithelial cells (Beas-2B), murine macrophage cells (RAW 264.7) and murine fibroblasts (3T3) were obtained (ATCC, Manassas, VA, USA). Beas-2B, RAW 264.7 and 3T3 cells were induced with vehicle, recombinant IL-13 (25 ng/ml), recombinant IL-4 (20 ng/ml) or recombinant TNF-α (20 ng/ml) (mouse or human, R&D Systems, USA). Bronchial epithelial cells were also induced with culture supernatants of IL-13 induced or uninduced macrophages or different concentrations of 12-S-HETE.
Lungs were disrupted with 70-μm cell strainer (Becton Dickinson, San Diego, CA, USA) to make single cell suspensions41,42 which were stained with surface markers such as Gr-1, CD11b, CD11c, CD3 (BD Biosciences, USA), Siglec F (R & D systems, USA), and F4/80 (Abcam, USA) or respective isotype control antibody (BD Biosciences, USA, Abcam, USA) followed by staining with 12-LOX antibody after permeabilization. The stained cells were acquired with FACScalibur (Becton Dickinson) and analysis was performed with softwares such as Cell Quest (Becton Dickinson) and FlowJo.
To stain the bronchial epithelia with JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide) dye, dual-emission potential-sensitive probe, Beas-2B cells were induced with culture supernatants of murine macrophages that were induced with 25 ng/ml IL-13 for 24 hrs and stained with JC-1 (Molecular Probes) as per manufacturer's instructions. In control experiments, Beas-2B cells were directly induced with protonopore, lipid soluble weak acid carbonyl cyanide m-chlorophenylhydrazone (cccp) which destroys mitochondrial membrane potential. JC-1 dye has dual staining properties as it forms red aggregates in a cell containing healthy mitochondria which have high membrane potential or it forms green monomers in a cell containing damaged mitochondria which have less membrane potential. The JC-1 stained cells were acquired and analyzed with FACScalibur (Becton Dickinson) equipped Cell Quest software to estimate mean florescence intensity of red aggregates (cells have healthy mitochondria).
All results except AHR and in vitro data are shown as quantile box plots (GraphPad Prism 5.0) which indicated median as a line across the middle of the quantile box, 25th and 75th quantiles as the ends of the box and 10% and 90% quantiles as the whiskers extending from either end of the box. Results of AHR, in vitro and supplementary data are shown in mean ± s.e.m. Two-tailed Student's t test was used to determine the statistical significance of the differences between paired groups whereas one-way analysis of variance was used to compare multiple groups with JMP software and was further evaluated with a nonparametric Mann-Whitney rank-sum test wherever appropriate.
U.M. designed, conducted, supervised, and interpreted experiments and wrote the manuscript; R.R. performed animal experiments, biochemical assays, ELISA, and qRT-PCR; T.A. performed airway physiology and imaging experiments; S.K. performed animal (C57BL/6) experiments; G.D.L. performed TEM experiments; S.S. performed in vitro experiments; A.K.D. supervised TEM experiments; S.B. supervised genetic knockout experiments; S.B., A.A. and B.G. provided conceptual support, interpreted experiments, and critically reviewed the manuscript; all authors reviewed the final manuscript.
Supplementary information
This work was supported by grants BSC 0116 and MLP 5502 from the Council of Scientific and Industrial Research, Government of India. We thank Professor Surendra Kumar Sharma (All India Institute of Medical Sciences, Delhi) for providing the human BAL and sputum samples. The helps provided by Dr. Satish Kumar Devarapu, Dr. Vijay Pal Singh, Mr. Younis Ahmad Butt, Ms. Sucitha Singh, Dr. Jyotirmoi Aich, Mr. Manish Kumar, Ms. Lipsa Panda, Mr. Ashish Jaiswal and Ms. Rituparna Chowdhury are greatly acknowledged.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers. |


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Author: Adrian Cartwright


text:
Tapulous ends the Tap Tap Revenge franchise
Tapulous has decided to end the Tap Tap Revenge franchise, which was extremely popular when during the first couple years of the Apple App Store launch. Tapulous said that the game's servers will be closed starting at the beginning of February, according to UberGizmo.
Tapulous is shutting down the game due to the changes in the mobile and music markets. If you still have the game downloaded on your phone, you can continue to use it.
If you reset your phone, then you will likely lose the songs that you may have purchased and downloaded without being able to restore them. The Android version of the game is also shutting down.
This article was written by Amit Chowdhry. You can follow me at @amitchowdhry or on Google+ at +AmitChowdhry


text:
View Liz Berg's profile |
Fist-pumping, air-drumming freeform mayhem. Caffeinated rock, experimental pop, oddball beats, and novelties.
Also available as an MP3 podcast. More info at our Podcast Central page.
<-- Previous playlist | Back to Liz Berg playlists | Next playlist -->
Listener comments! | |
Mon. 1/6/14 9:01am
Happy Monday Liz and Lizners | |
Mon. 1/6/14 9:03am
hello :) | |
Mon. 1/6/14 9:06am
Sem Chumbo:
Well, that was wonderfully lush. Now this is labial fricative if I ever heard it. Good morning. | |
Mon. 1/6/14 9:06am
Ken From Hyde Park:
Always room for more dogs. | |
Mon. 1/6/14 9:07am
Guido (Cologne):
Hi Liz! I have a serious respect for Carlton Melton! They're just jamming and almost no editing. My last experience in a jamming band was editing like mad in order to get something enjoyabele out of it. "Always Even": recommendable album. | |
Mon. 1/6/14 9:07am
Hi everyone! | |
Mon. 1/6/14 9:11am
Liz Berg:
Good morning everyone and welcome to this sleepy grey Monday! | |
Mon. 1/6/14 9:14am
good monday to everyone! hope it's good. | |
Mon. 1/6/14 9:20am
Well, these are nice sounds indeed. | |
Mon. 1/6/14 9:41am
Liz Berg:
Can't wait for the winter olympics to start... there's nothing like watching grown men sweeping a broom over the ice yelling at a little puck, eh? | |
Mon. 1/6/14 9:49am
Guido (Cologne):
Ice hockey over here had an signifcant increase in popularity during recent 20 years, since the major Cologne soccer team became notoriously unsuccessful. | |
Mon. 1/6/14 9:49am
love me some curling, liz. | |
Mon. 1/6/14 9:50am
Is it true that Pussy Riot is playing at the opening ceremony of the Olympics? | |
Mon. 1/6/14 9:53am
Liz Berg:
I wish Pussy Riot was playing at the opening ceremony! Perhaps they'll pull another one of their crazy demonstrations... | |
Mon. 1/6/14 9:54am
Sem Chumbo:
Liz: for me, the winter Olympics are all about spandex-clad lugers risking life and limb lying on their backs hurtling downhill thru banked S bend after S bend at 90 +mph. It is a try-out for the Darwin Awards, the really big show. | |
Mon. 1/6/14 9:55am
Liz Berg:
I also have a guilty pleasure in the figure skating dept: high drama and bad costumes! | |
Mon. 1/6/14 9:55am
I actually dig curling as well. Would love to try my hand at it some time. | |
Mon. 1/6/14 9:57am
Sem Chumbo:
@NGH: how's you back? Granite curling stones weigh 40 pounds, and one has to lift them off the ice at some point:( | |
Mon. 1/6/14 9:58am
Morning Liz! I will shamefully admit I enjoy figure skating, especially the men. Johnny Weir is my hero. Is he competing this year? | |
Mon. 1/6/14 9:58am
guess i can never curl, sem. oh well. | |
Mon. 1/6/14 9:58am
mining the fma like whoa | |
Mon. 1/6/14 9:59am
Liz Berg:
I heard a rumor that Jonny Weir will be a commentator! EEP! | |
Mon. 1/6/14 10:02am
Considering my flightcase (for my vinyl recs) is 35 pounds, and my laundry bag I schlep once a month to the laundromat is 30-40 lbs, I think I'll be find. It's my knees that our my weakness. Dug the Proligate track. | |
Mon. 1/6/14 10:03am
Sem Chumbo:
@common: how about biathlon? Cross country skiing and gunplay. Gotta love that! | |
Mon. 1/6/14 10:06am
-20 ℉ / -29 ℃ on the isthmus today. Enough to make your face hurt. | |
Mon. 1/6/14 10:07am
Sem Chumbo:
@NGH: fyi 2:25 curling lesson www.youtube.com... | |
Mon. 1/6/14 10:08am
Then again, I suck at shuffleboard and related games, but I still like playing. | |
Mon. 1/6/14 10:10am
Liz Berg:
Oh yeah, Sem! Biathlon is nuts! I didn't believe it was real the first time I saw it. | |
Mon. 1/6/14 10:10am
@ Liz - uh oh, I wonder how Jonny will do off the ice? Could be really funny or really annoying. Another winter olympics guilty pleasure is men's speed skating. Apolo Ohno is hypnotic to watch gliding in circles. | |
Mon. 1/6/14 10:13am
If my furlough from my job continues into February, I can watch the Olympics, but my bank account will be getting mightily slim. | |
Mon. 1/6/14 10:20am
Gotta jet! Thanks Liz! | |
Mon. 1/6/14 10:20am
Sem Chumbo:
Liz:biathlon came from a Norwegian army exercise in the 1860's. Seems an odd addition to the Olympics; I think I'd prefer a maple tree tapping/sauna sitting variant. | |
Mon. 1/6/14 10:20am
@Sem: i don't like guns. but i love to watch skiing. | |
Mon. 1/6/14 10:22am
"reign"? but today is RAIN. and since it's WOODSIST, is that FOXYGEN guy on woodsist? i think EFDavies played a song of his but can't remember if it was good or not. wait, why don't i just go to archive? never mind. -- happy new year, liz, and to all. | |
Mon. 1/6/14 10:23am
Kevin Morby ... Good! | |
Mon. 1/6/14 10:24am
No alt.side parking today, so you get my av. | |
Mon. 1/6/14 10:30am
Hey Liz, you are the best. So happy to be listening live. I usually teach on Monday mornings (studio class) and try to listen live but students are so square--end up having to turn music off. Happy Monday! ew | |
Mon. 1/6/14 10:32am
Liz Berg:
Ha ha, thanks for tuning in live, Emily! I'm sure one day WFMU will rub off on a few of your students! | |
Mon. 1/6/14 10:35am
Chris from DC:
Hey Liz. Liking the NZ segment here. | |
Mon. 1/6/14 10:40am
i thank n.carolina writer FRED MILLS and n.zealand writer WLYee for turning me on to the CHILLS, 1988 (?). and thank you MATADOR for "brave words". | |
Mon. 1/6/14 10:45am
HI Liz, HI KIddles.,,lovely to be here live also,,, wFMu should be required reading for all well rounded students no matter how square.. | |
Mon. 1/6/14 10:50am
because it is:
Students don't like FMU? Can't help but wonder what your teaching in your studio emily...how to properly starch your granny panties? | |
Mon. 1/6/14 11:00am
Love "Miles, Miles, Miles" by Kevin Morby. Pursuit of this gives me excuse to face a bleak day. | |
Mon. 1/6/14 11:03am
Pink Panther! Quintron! | |
Mon. 1/6/14 11:04am
Ken From Hyde Park:
Happy Plough Monday, all. Back to work and all that. | |
Mon. 1/6/14 11:12am
No plow could break ground hereabouts. It's rock hard. | |
Mon. 1/6/14 11:13am
cant find this song on youtybe :( | |
Mon. 1/6/14 11:13am
Ken From Hyde Park:
Snow Plow Monday would be more like it. | |
Mon. 1/6/14 11:14am
I love this Saâda Bonaire | |
Mon. 1/6/14 11:16am
Found it! | |
Mon. 1/6/14 11:26am
Hello Liz, nice show! What is that song that sounds while you speak with that amazing bass? Thank you :) | |
Mon. 1/6/14 11:28am
Why don't you play the whole track??!! (Launderette) | |
Mon. 1/6/14 11:34am
Thank you, 99records | |
Mon. 1/6/14 11:37am
Liz Berg:
One day I shall play the entire track... it's one of my faves! | |
Mon. 1/6/14 11:47am
Are there only 2 tracks on this Blow Mind single? Both songs I've heard so far are great! | |
Mon. 1/6/14 11:48am
Liz Berg:
Yep, 2 tracks, both awesome. Blow Mind were from France, this was recorded in 1970. | |
Mon. 1/6/14 11:49am
"they're coming"? ooh so drug-addled. but i'm a straight-edge. what to do? | |
Mon. 1/6/14 11:50am
@Herb - live vicariously through Blow Mind? | |
Mon. 1/6/14 11:53am
thanks PKYN, but i'm just gonna say "yes" | |
Mon. 1/6/14 11:54am
@herb break edge for 2 minutes? | |
Mon. 1/6/14 11:56am
Guido (Cologne):
@herb watch a cheesy SiFi-movie | |
Mon. 1/6/14 11:56am
That calls to mind a new favorite French word: www.wordreference.com... | |
RSS feeds for Liz Berg's show: Playlists feed | MP3 archives feed
| E-mail Liz Berg | Other WFMU Playlists | All artists played by Liz Berg |


text: AVAILABLE AS AN ONLINE EXCLUSIVE AT NET-A-PORTER.COM. Drawing design inspiration from Japan, tokidoki's "criminally cute" cartoon characters have a cult following worldwide. We love this Karl Lagerfeld figurine. Dressed in the sartorial icon's signature black, with a silver tie and twinkling diamantés, this collectable piece will make a playful addition to any style-savvy home.


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Sci fi/Fantasy image by
Christina DoanOracle is my story that I have been working on for a year or so now. Basicly, my characters are on a quest to kill Prince Vlad Dracul and save the ambassadors daughter from becoming his bride. What they will soon realize is there is much more then they will ever bargain for. Im thinking on turning this into a comic, since its already a written story, it would be interesting to see it turn into a graphic novel. The Characters above are as followed... The woman is Helena Valmond. She is the main character and your local heroin who guilds you through the story. She is the first and only female to the Holy Order of the Knights who is signed by the Pope and Cardinal to be a Slayer of all things 'unholy'. Shes very bullheaded, strong witted, and highly trained for battle. The blonde man is Lucian, a vampire on whom they catch spying one day. He is kept around and alive because he has convinced Helena that he will become useful to them. He is a french cocky nobleman who likes nothing more then to toy with mortal emotions, and he has been around since the Middle Ages. The man in brown with the stubble is Demitri Van Buran, a slayer originating from the Vatican in rome. He usually deals with werewolves, and was shipped to Romania by the council to assist Helena in the rescue. He is friendly, but isint very good at cracking a joke. Hes the type of slayer who-like Helena- Doesitn show much mercy in his work. The red haired in the corner is Adon Blackwood. He is a decendent from a family of knights and noble warriors in the past and in his generation of the Victorian era- He is the ladies man- and the Casanova of that time age. Adon's bed time deeds have been so scandilise, he has given a priest a heart attack, and had caused nuns to start wearing iron chastity belts. He is quite charming, nomatter how unappealing he may seem. The man with the black hair in the back is Louis Buxton, a Lieutenant who has been sent from Paris to Romania, working for the military in Romania. He is a polite, wemm mannered younge man who see's goodness in everyone but himself.
Published More than a year ago
Category Horror
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text: seen quite a few threads ask this but no answers yet im just after a simple tilting wall mount for around £100 - £150 for the LG RZ42PX11 that ive ordered .I know theres a problem with a number of mounts because of where the scarts are positioned. Any PX11 owners out there that can recommend one? Tia


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retail: $60
Home Depot has the 3-Pack of Rust Oleum 18 oz. NeverWet Waterproofing Spray Kit for $51 with free shipping. 2-step spray process works on nearly any surface to create a water-repellent coating; each kit covers 10-15 sq. ft. The CheckOut spotlights this new superhydrophobic product.
Same price on Amazon.
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Why You Should Speak at Conferences & How to Overcome the Barriers
Build credibility and your personal brand by presenting at your next conference or trade show. We outline the benefits to speaking and describe how to overcome any barriers.
Build credibility and your personal brand by presenting at your next conference or trade show. We outline the benefits to speaking and describe how to overcome any barriers.
Digital is everywhere, so it is no surprise that almost every trade show exhibit has some sort of digital content. Have you considered the positives and negatives of incorporating technology into your trade show exhibit?
Technology is an integral part of our lives and is being used more and more in trade shows and events everywhere. Here is a list of the top technology trade shows and events for 2017.
A listing of notable upcoming U.S. Health Care trade shows.
The landscape of trade shows has changed and with that the type of skills needed to staff the booth. What skills do your booth staffers need in the era of digital?
Where do business-to-business exhibitions rank among most popular Sales and Marketing communications tactics in the 2016 Changing Environment of Exhibitions Study? The answer might surprise you.
Even if your trade show or buying fair is not Olympic sized there are many things in common with large-scale events like the Olympics. What can we learn from Rio 2016?
Audio Visual equipment has become a necessity on the trade show floor. We have 18 tips for you to prevent AV panic.
What is Digital Marketing, why is it important, and how can I learn more about it to incorporate it into my trade shows and events?
More and more exhibitors are seeing the value in serving their attendees food and beverages at trade shows and events. When determining your menu, there are 7 types of food we recommend you avoid.
Even the most carefully chosen booth staffers can end up hurting your event, and then, it's time to say goodbye. Here are 9 reasons you should fire your booth staffer.
Event and Trade Show organizers have the 5th most stressful job in the nation. Don't worry, we've got 10 stress-fighting hacks for you.
Take a look back at the biggest event marketing trends of 2015 and see how they can make an impact on this year's event marketing plans.
Top tech events in 2016 and 2017. Review this list to find shows where you can find new products, get training or determine whether to exhibit in the future.
Learn 12 things to avoid when planning trade show and event promotions. Make sure you tie your promotions to your brand or message and avoid low quality.


text:
Genre: Sitcom
Premise: Hayden Fox is a football coach, the head coach of Minnesota State University's Screaming Eagles for much of the series' run. He has to contend with a team that has seen better days, a couple of none-too-bright assistant coaches, a partner who isn't half as obsessed with football, and all the rest of the situations that come along in Big Ten athletics. For the show's final two seasons, Fox coaches an expansion team in the NFL.
S01, E13 | Dauber's Blowout | ||
S01, E12 | Hoot, Hoot Hike | ||
S01, E11 | Whose Team Is It Anyway? | ||
S01, E10 | Define Romance | ||
S01, E09 | I'm Sorry I Told You My Wife Was Dead |


text:
Providing quality healthcare since 1939
Since Utah Valley Hospital (formerly Utah Valley Regional Medical Center) first opened its doors in 1939, we have been committed to meeting healthcare needs of individuals and families in Utah County and Central and Southern Utah.
Utah Valley Hospital became the first fully staffed 24-hour emergency center south of Salt Lake City, and 98 physicians enjoyed medical staff privileges at the hospital.
The hospital celebrates its 75th anniversary, and a major hospital replacement project is announced to eventually replace the East Tower and provide the latest technology and medical services to the community.
The name of the hospital is officially changed back to Utah Valley Hospital. As Utah Valley Hospital grows, it continues its evolution as a place of learning, a place of caring, and a place of healing.
The Utah Valley Hospital Primary Children's Network opens, offering the best pediatric care for patients and their families, close to home.
Get Care Now
Get care for low-level urgent conditions through a video chat with a provider, 24/7, on your computer or mobile device.Learn More.
View wait times and save your place in line at an Intermountain InstaCare location near you.
If this is an emergency please go to the nearest emergency room or call 911.
Still not finding what you are looking for?


text:
BACARDÍ Rum Launches "Untameable" Campaign
- New, bold "BACARDÍ Untameable Since 1862" campaign launches globally, unites brand and products
- Evocative print, digital and TV ads inspire consumers to unleash their "Irrepressible Spirit" to live
boldly and passionately - Authentic, artisan, hand-drawn bat logos from 1900s inspire new design
Hamilton, Bermuda, November 18, 2013––Everyone has a story. The BACARDÍ brand and family's story can be summed up with one word: "Untameable." With more than 150 years of uncompromised passion triumphing against extraordinary odds, BACARDÍ is the rum brand with tales where the truth is better than any fiction. Fearlessly honoring its legacy, the brand with "Irrepressible Spirit" written in its DNA, today announced the launch of "BACARDÍ Untameable Since 1862," a new global marketing campaign that tells the stories behind BACARDÍ rum, its origins in Cuba and inspires consumers to embrace life and live boldly to showcase their own "Irrepressible Spirit."
The television, digital and print ads embody the true grit and determination that sustained the rum brand and helped it thrive. The advertising will feature headlines such as, "We Remember Prohibition – It was a Blast;" "Some Men are Kicked Out of Bars – Others are Kicked Out of Countries;" and "Earthquakes, Fire, Exile, Prohibition – Sorry Fate, You Picked the Wrong Family."
The ads offer snapshots of the BACARDÍ history during prohibition, exile from Cuba, and showcase how the Bacardí family had the original irrepressible spirit to overcome earthquakes, fire, war and revolution—none of which could defeat their spirit, because "True Passion Can't Be Tamed." The campaign is designed to engage millennial consumers to pursue their passions no matter what—much like the Bacardí family did—encouraging camaraderie and strength of character.
"BACARDÍ Untameable Since 1862" will be supported by 30-, 60-, and 120-second TV advertisements, as well as robust outdoor billboard, print and digital executions. The television spots—named "Procession," because of the frame-by-frame timeline and sequence of events viewers experience—were directed by award-winning Dante Ariola, star actor Jordi Mollà and has voiceover of actor Jason Isaacs. The ads, filmed on location at the UNESCO World Heritage site of Ouro Preto, Brazil, are highly stylized and have a blockbuster movie feel. The ads exude a realism that commands attention—engaging consumers to feel that with every sip, the BACARDÍ brand story is their own.
"BACARDÍ is known for its passionate drive, regardless of circumstance, convention or expectation. The campaign pays tribute to the brand's enduring popularity, continuing ingenuity and connects with consumers as it inspires them to ignite the bold, 'Irrepressible Spirit' within us all," said Dmitry Ivanov, Senior Global Category Director of Rums for Bacardi. "Taking a global creative approach with the 'BACARDÍ Untameable Since 1862' campaign allows us to focus all of our marketing resources against a single powerful idea; an idea that's completely ownable to BACARDÍ as it is rooted in the brand's history and the Bacardí family's attitude to life."
The BACARDÍ portfolio of light and dark rums globally will be united under one BACARDÍ creative look and feel. Every market in the world will have the same BACARDÍ swagger in its branding, marketing campaigns, advertising and visual identity for these rums. All markets will also feature the bigger-than-life, "untameable" BACARDÍ attitude.
Along those lines, the new campaign pays tribute to Don Facundo Bacardí Massó, who had the vision, 151 years ago, to blaze a new path and add steps never before used in rum making to create rum as the world knows it today. With the same pioneering soul of its founder, and in remembrance of its humble origins and beginnings in Santiago de Cuba in 1862, BACARDÍ rum will face the future with a bat logo inspired from the past.
Looking deep into the BACARDÍ archives to uncover the treasured, authentic, artisan bat designs over the past 151 years, the new logo is a creation inspired by BACARDÍ bat hand-drawn designs from the early 1900s. It stays true to the integrity of the original symbol, which in Cuban and Spanish heritage represents good luck, good fortune and family unity.
The BACARDÍ word mark has also been updated, influenced by the Cuban Art Deco style from the late 1920s to early 1930s. The most famous example of this Art Deco style is the former Bacardi sales office in Havana known as El Edificio Bacardí (The Bacardí Building). To this day, the 11-story building remains crowned by a BACARDÍ bronze bat and emblazed with the Art Deco stylized typeface.
"This campaign ushers in a new era for BACARDÍ rum, with a deeper focus on its provenance to showcase the unfailing determination of the Bacardí family and invites consumers to share in it," said Ivanov. "This idea infuses the BACARDÍ brand with the authenticity of its origins, celebrates the incredible quality and craftsmanship of our rums and showcases a universally resonant attitude. In essence, 'BACARDÍ Untameable Since 1862' is a salute to the Bacardí family's incredible heritage, to pursuing your passions, and to an extraordinary rum."
Using creative digital formats, fans will be able to discover and share the "Irrepressible Spirit" of BACARDÍ rum for themselves, with access to artifacts from the brands 151-year history and behind-the-scenes photography and video footage from the ad shoot, offering a truly immersive experience.
"BACARDÍ Untameable Since 1862" will launch in the United States and Mexico on November 20, and India and Australia in December, followed by other markets in spring 2014.
For more information on "BACARDÍ Untameable Since 1862," BACARDÍ rum and cocktail recipes, visit bacardi.com.
About BACARDÍ Rum – The World's Most Awarded Rum
In 1862, in the city of Santiago de Cuba, founder Don Facundo Bacardí Massó revolutionized the spirits industry when he created a light-bodied rum with a particularly smooth taste – BACARDÍ. The unique taste of BACARDÍ rum inspired cocktail pioneers to invent some of the world's most famous recipes including the BACARDÍ Mojito, the BACARDÍ Daiquirí, the BACARDÍ Cuba Libre, the BACARDÍ Piña Colada and the BACARDÍ Presidente. BACARDÍ rum is the world's most awarded spirit, with more than 550 awards for quality, taste and innovation. Today, BACARDÍ rum is made in Puerto Rico and Mexico where it is crafted to ensure the taste remains the same today as it did when it was first blended in 1862. www.bacardi.com


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For nearly 30 years, Whitwell Landscaping has been serving the metro Atlanta area. We provide high-end landscape design, installation, tree removal and maintenance services to residents, homeowner's associations and commercial properties. We have completed hundreds of projects over the years with the same attention to detail and quality that keeps our customers coming back and referring us to their friends and families. With over 30 years of combined experience between our skilled team of landscape architects, craftsmen and project managers, we can meet our goals of high quality, precision and integrity with each and every project we undertake.
Our services include:
- Landscape Design
- Lawn maintenance
- Cleanup and removal
- Correcting drainage and erosion
- Landscape upgrades and renovations
- Hardscape construction
- Bobcat and trenching service
-.. Grading
- Mulch installation
- Sod installation
- Tree and plant installation
- Water features, patios and outdoor fireplaces
We have a flat yard so major drainage issues whenever there's significant rain. Joe recommended the Flo Well system, installed the basins, re-routed the gutters to it as well, and put in all new grass. Him and his team were knowledgeable, hardworking, on-time and easy to work with. I especially appreciated Joe's attention to detail. I highly recommend Whitwell Landscaping!
Project Details
Area Buford, GA
Cost $5,000 - $9,999
Date March 2017
Posted: 3/27/2017 • Link to this review
I contacted Joe and his team to fix a serious drainage issue in my front yard. He was very responsive to my initial request, developed a great and reasonably priced plan to solve the issue, and even acted as my advocate to accelerate the HOA approval process. As soon as I got the green light from the HOA, Joe's team began work the next day. They worked quickly, with incredible attention to detail and an eye for aesthetics, and removed 2 trees and their roots, graded my entire front yard, and installed sod, bushes and mulch all within a few days. The outcome certainly exceeded my expectations, and I'm so happy that I finally have a beautiful front yard. I will definitely use them again when I'm ready to tackle my backyard. Thanks, Joe!!
Project Details
Area Duluth, GA
Cost $3,000 - $4,999
Date December 2016
Posted: 2/03/2017 • Link to this review
We needed help with erosion issues as our home is on a slope. Our yard was in really bad shape before Joe and his team fixed that. They created a new river bed, and planted various plants down the slope to help with erosion. He also pruned and mulched the entire yard. Joe was wonderful to work with. He came in and evaluated the yard, came up with a proposal that worked for us, and started the work immediately. Whitwell landscapers are perfectionists in everything they do. Our yard never looked the way it looks now. We are very pleased with the work done and will definitely be hiring them to do more work for us in the future.
Project Details
Area Suwanee, GA
Cost $5,000 - $9,999
Posted: 9/30/2016 • Link to this review
I had an overgrown Juniper bed that destroyed the looks of my backyard. Within 3 hours Whitwell Landscaping changed that. Thanks
Posted: 8/20/2016 • Link to this review
I had a drainage problem on one side of my house. Water was going under the foundation of my ranch home causing my wood floors to crown. Joe landscaped the side of my house to allow rain water to drain to the creek in the back of my house. Excellent work! Would use Joe Whitwell again! He knew exactly what I wanted! We will be able to mow the area and did not need all those expensive french drains!! Thank you!!
Project Details
Area Alpharetta, GA
Cost $1,000 - $2,999
Date May 2016
Posted: 5/14/2016 • Link to this review
2012, 2013, 2014, 2015, 2016
Whitwell Landscaping is fully licensed and insured.
* 24-Hour Service Available
[PDF] Whitwell Landscaping Testimonials
[PDF] Whitwell Landscaping Landscape Calendar
[PDF] Whitwell Landscaping Service Area
Whitwell Landscaping offers a variety of drought resistant plants and many ways we can help your landscape use less water and, of course, stay green!


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A Day Away was born in 1996 to Titusville native and Eco Tourism enthusiast Laurilee Thompson. At that time it was known as "Osprey Outfitters". Soon after its birth the business received a new name as it changed hands from LaurieLee to Mr. Rick Shaffer - A Day Away Kayak Tours - with its base of operation being the Merritt Island National Wildlife Refuge. Rick was training in the medical field and unable to focus his full attention on the struggling little business.
Now enter the current owners, Mike and Elisabeth Mahan. In late 2004 Mike came across Rick while looking for kayaks to use within an outdoor adventure ministry. After the purchase of some kayaks and a short conversation, Mike came home very surprised to be the new owner of A Day Away Kayak Tours. His initial plan was to involve some students and have some fun using the business as a tool for learning.
In Early 2005 through a series of life changing events Mike began working the business himself with a small group of friends. And, by mid-2005, A Day Away Kayak Tours was again up and running full time offering tours on the Merritt Island National Wildlife refuge.
What an adventure Mike and Elisabeth have been on since those early days in 2005. Constant growth and evolution have been the norm...from running one very old van and trailer with 20 seats and 2 staff, to our current fleet of 4 vehicles; 4 trailers; 96 seats (replaced annually); a small motorized fleet and a 6 passenger pontoon boat. A Day Away has also grown to employ a seasonal staff of 20-24 guides, operations manager and office staff all of which are more like a family than a group of employees. In 2015 A Day Away was awarded the Certificate of Excellence from Trip Advisor.
In our rushed electronic world which often leaves people nature deprived, Mike and Elisabeth have made the goal of A Day Away to offer quality tours with quality equipment led by people of excellence ensuring every participant a reconnect with nature that leaves them in awe of the amazing creation surrounding them.


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How to Scale-Up a Wet Granulation End Point Scientifically: Volume 1
By: Michael Levin (author)PaperbackMore than 4 weeks availability
How to Scale-Up a Wet Granulation End Point Scientifically provides a single-source devoted to all relevant information on the scale-up of a wet granulation end point. Contents include a general description, problem identification, and theoretical background with supporting literature, case studies, potential solutions, and more. By outlining issues related to scale-up and end-point determination, and then using practical examples and advice to address these issues, How to Scale-Up a Wet Granulation End Point Scientifically is a valuable and essential resource for all those pharmaceutical scientists and technologists engaged in the granulation process. * Thoroughly referenced and based on the latest research and literature* Part of the Expertise in Pharmaceutical Process Technology Series edited by internationally respected expert, Michael Levin* Illustrates the most common problems related to scale-up of a wet granulation end point and provides valuable insights on how to solve these problems in a practical wayCreate a review
About Author
Michael Levin has over 30 years of experience in the field of pharmaceutical scale-up, instrumentation and process optimization. He has edited a "Pharmaceutical Process Scale-Up book, and has contributed chapters on "Tablet Press Instrumentation and "Wet Granulation: End-Point Determination and Scale-Up in the Encyclopedia of Pharmaceutical Technology. Michael holds a M.Sc. degree in History and Philosophy of Science from Hebrew University of Jerusalem and Ph.D. in BioMathematics (1980) from University of Washington in Seattle. His Post Doctorate studies were at University of Houston, Department of Mechanical Engineering. He participated in numerous wet and dry granulation and tableting projects in major pharmaceutical companies as a provider of process analytical instrumentation, conducted many seminars, performed numerous consulting services and wrote expert reports to clients in the pharmaceutical industry.Product Details
- publication date: 20/10/2015
- Format: Paperback
- Number Of Pages: 88
- weight: 159
Delivery Information
- Saver Delivery: Yes
- 1st Class Delivery: Yes
- Courier Delivery: Yes
- Store Delivery: Yes


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Rad Cat Raw Diet FAQ
At Rad Cat we love all of the questions we receive from so many caring people. Here are some of the questions we are asked most often. Please email us if you have a question we have not answered. How Much Rad Cat Should I Feed My Cat?
Why Does Rad Cat Use Powdered Eggshell Instead Of Ground-In Bone?
Why does my cat throw up after eating the lamb, beef or venison? |
How Much Rad Cat Raw Food Should I Feed My Cat?
How Long Does Rad Cat Stay Fresh in the Refrigerator?
Typically, our products will stay fresh for 3-4 days in a refrigerator kept between 37-40 degrees. |
Why Has Rad Cat Chosen To Use Container Packaging Instead Of Packing In Chubs Or Forming Patties, Nuggets, or Medallions?
Do I Need To Add Supplements To Rad Cat?
Do I Need To Worry About Samonella and E.Coli With Rad Cat Raw Cat Food?
We pride ourselves in the quality of our products, which includes a very tight quality control program. All of our batches are tested by an independent laboratory for total bacteria, Salmonella, and E.Coli O157:H7. Even though we test our products before they leave our plant, the first level of our quality control begins with the sources of our meats - the control of bacteria begins at the farm. We only work with vendors that we feel comfortable about their quality standards. Products that come from sources that provide free-range meats or have organic certification are typically an assurance of overall higher quality and higher standards, which includes testing and methods to assure safe handling, packing, and shipping. Working with responsible farms in conjunction with our strict product testing procedures provides very safe, healthful products. Our poultry ingredients (the thigh and leg, hearts and livers) undergo high pressure pasteurization before they reach our facility. This "kill step" is recognized by the FDA for many food products, as a way to eliminate pathogens from ready-to-eat foods. It will only kill pathogens and spoilage organisms, such as salmonella, E.coli, Listeria and Campylobacter. As poultrydoes not contain "beneficial" bacteria, there is no concern about these bacteria being affected. This is simply another step to make sure our products are free from pathogens. Please visit our About HPP page, under Education for more information. We also use Ozone Technology in our processing which is an additional insurance that our meats and poultry are free from contamination. Ozone is used environmentally, where it is part of our cleaning process. Ozone is a natural oxidizer that kills bacteria on contact, having no residual effects. It makes our production area smell fresh, like right after a fresh spring rain and keeps our entire environment bacteria and mold-free. In nature, that scent is actually ozone! Please see our Safety and Quality page for more information on how we assure our products are safe. |
What Does Rad Cat Use as a Source of Taurine?
Is It Okay That My Cat Doesn't Drink As Much Now That He/She Is Eating Rad Cat?
Is Rad Cat An Option For My Cat With Renal Disease & FLUTD?
Where Does Rad Cat Raw Cat Food Source Their Meat?
Why Does Rad Cat use Powdered Eggshell instead of ground bone?
What Is The Best Way To Transition My Cat To A Raw Diet?
Is Rad Cat Good For Overweight Cats And Cats With Diabetes?
Is Rad Cat Good For My Cat With IBS/IBD (Irritable Bowel Syndrome/Disease)?
As irritable bowel syndromes and diseases may have many causes, a healthy diet to support their digestion and immune system is paramount. Rad Cat is formulated to be the most easily digestible raw cat food and to provide support to maintain a healthy digestive tract. This issue was the driving force behind the formulation of Rad Cat. Click her for Juno's story! Raw diets are easily digestible due to the presence of naturally occurring enzymes in the meat and high moisture content. At Rad Cat, we add gelatin to our diets to enhance the digestive process. Gelatin is high in the amino acid glycine (as well as many others), which increases the hydrocloric acid (HCl) content of the stomach, as well as other gastric secretions. This is very beneficial for cats that are eating a high protein diet. Cats generally have very acidic stomachs, but many cats come to eat raw after being ill and have compromised immune systems and sluggish digestion. Cats without digestive problems can also benefit from the presence of the added amino acids that gelatin provides. See the blog post about the many benefits of gelatin!
When ground bone is used in cat food, in order to maintain a proper calcium:phosphorus ratio, the diet can consist of upwards of 10% ground bone. This can lead to constipation in many cats when fed this much bone on a regular basis. |


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Fullerton station (CTA)
Location | 943 West Fullerton Avenue Chicago, Illinois 60614 |
Coordinates | 41°55′30″N 87°39′10″W / 41.925051°N 87.652866°W | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Owned by | Chicago Transit Authority | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Line(s) |
Purple Line weekday rush hours only
Platforms | 2 island platforms | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Tracks | 4 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Construction | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Structure type | Elevated | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Disabled access | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
History | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Opened | May 31, 1900 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Rebuilt | 2006–09 (station reconstruction) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Traffic | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Passengers (2015) | 4,233,730[1] 1.9% | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Rank | 6 out of 143[a] | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Services | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Fullerton is an 'L' station on the Chicago Transit Authority's Brown and Red Lines. Purple Line express trains also stop at the station during weekday rush hours. It is an elevated station with two island platforms, serving four tracks, located in the Lincoln Park neighborhood in Chicago, Illinois. Brown and Purple Line trains share the outer tracks while Red Line trains run on the inner tracks. As well as being an important transfer station, the station serves the Lincoln Park Campus of DePaul University.
Fullerton Station opened in 1900 as an express/local stop on the Northwestern Elevated Railroad.[2] In the 1920s, the station's platforms were lengthened to serve eight-car trains, but due to surrounding buildings the platforms were lengthened in different directions; the northbound platform was extended northward, and the southbound platform was extended to the south. In 1949, an additional set of exit stairs was added at Fullerton as well as a 15 feet (4.6 m) tall supervisor's booth on the platform. Off-peak shuttle service on the Ravenswood Line ran from Kimball to Fullerton until 1961, when the shuttles were cut back to their present-day terminus at Belmont.
In 1989, Evanston Express trains began stopping at Fullerton due to increased ridership. The original station house was damaged by a fire in December 1996; the police suspected that the fire was the result of arson. The station house was reopened in February 1997 with only one ticket agent's booth. However, the single booth could not handle the high passenger load at the station, and a new station house was opened next to the original in 1998.
Demon Dogs[edit]
In 1983, the CTA decided to lease the space beneath Fullerton to a business. The CTA, led by chairman Michael Cardilli, granted the lease to Peter Schivarelli, a friend and former coworker of Cardilli's. Schivarelli built the hot dog stand Demon Dogs in the space. While Schivarelli paid for the construction of the stand, he owed a low rent on the property, and the CTA paid the utility bills for the building. The CTA ended this arrangement in 1998 upon its being made public, and Schivarelli sued the CTA as a result. A trial court ruled in favor of Schivarelli, but the CTA appealed the decision, and it was overturned by an appellate court. Demon Dogs remained open until 2005,[3][4] when it was removed during the station's reconstruction.
Brown Line Capacity Expansion Project[edit]
Fullerton station underwent reconstruction as part of the Brown Line Capacity Expansion Project. The new station has wider platforms and elevators to ensure that it meets ADA accessibility requirements. The new platforms are able to accommodate 10-car trains, allowing for future expansion of the Red Line.[5] Because of its importance, the station remained open throughout the entire project unlike other stations which face temporary closures. This will be achieved by the use of a temporary station house that opened on October 8, 2006.[6]
The renovation project has not been without controversy. The demolition of both the well known Demon Dogs hot dog stand and DePaul University's 1929 Gothic-style Hayes-Healy Athletic Center were unsuccessfully contested.[7][8] Elevators were placed into service on December 31, 2009, making Fullerton accessible for customers with disabilities.[9][10]
As part of the Red, Brown and Purple Lines, Fullerton is used by passengers travelling between Lincoln Park and the Loop, Ravenswood and neighborhoods on the North Side. Fullerton is served every seven to eight minutes by Brown Line trains, every five to eight minutes by Red Line trains on weekdays and every seven to ten minutes on weekends, and every 10 to 20 minutes by Purple Line trains during weekday rush hours. While the Brown Line ends service to Fullerton at 1:30 a.m. on weekdays, Red Line trains run 24 hours a day, so the station is open at all times. In 2007, 3,496,676 passengers boarded at Fullerton, a 1% increase from 2006. These figures made Fullerton the second-busiest station on the Brown Line, the fourth-busiest station on the Red Line, and the second-busiest station on the Purple Line.
The fare controls at Fullerton are located in a new station house on the south side of Fullerton Avenue; the original station house was closed for renovation and was moved across the street and converted to an auxiliary farecard-only entrance. From the station house, staircases lead to the station's two island platforms. The east platform is used by northbound trains, and the west platform is used by southbound trains. Additional exit stairs lead from the platforms to the north side of Fullerton Avenue. The project's Full Funding Grant Agreement with the federal government required that the CTA complete the project by the end of 2009. The new station was completed on December 31, 2009.
Bus connections[edit]
- 11 Lincoln
- 37 Sedgwick
- 74 Fullerton
Notes and references[edit]
- ^ Due to possible double-counting of physically-connected stations, the CTA's official 2015 tally of stations was 146, but for ridership purposes reported having only 143 stations.
- ^ "Annual Ridership Report – 2015" (PDF). Chicago Transit Authority Ridership Analysis and Reporting. January 13, 2016. Retrieved May 29, 2016.
- ^ Fullerton. Chicago-"L".org (URL accessed October 7, 2006).
- ^ chicagoist.com. Gothamist http://chicagoist.com/2004/08/27/the_bill_comes_due_for_demon_dogs.php. Missing or empty
(help) - ^ http://articles.chicagotribune.com/2003-04-03/news/0304030250_1_cta-dogs-lease
- ^ Chigago Transit Authority Ravenswood (Brown) Line Expansion Environmental Assessment. Chicago Transit Authority
- ^ Fullerton Station Entrance to Temporarily Relocate on Sunday. CTA press release (URL accessed October 7, 2006).
- ^ Vanishing Chicago: The Gym. adoresixtyfour.com (URL accessed October 7, 2006).
- ^ Ben Joravsky Oops! They Did It Again. Chicago Reader, December 16, 2005.
- ^ "Customer Alert: Elevators at Belmont and Fullerton Stations Placed in Service". Chicago Transit Authority. Retrieved 2009-12-31.
- ^ "CTA to Open New Elevators at Brown Line Belmont and Fullerton Stations" (Press release). Chicago Transit Authority. December 28, 2009. Retrieved 2009-12-31.
External links[edit]
Media related to Fullerton (CTA) at Wikimedia Commons


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Frederica, Queen of Bavaria
(1825-1889), Wife of Maximllian IISitter in 2 portraits
by Franz Neumayer
albumen carte-de-visite, circa 1860
NPG x7880
by Joseph Albert
albumen carte-de-visite, 1860s-1870s
NPG x7881
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A New Interview as the Summer Blog Tour Shifts Into Fall
September 10, 2013
It's been a couple of weeks since my last post for the Summer 2013 Thieves' Quarry Blog Tour (yes, it's September, but it's still summer officially . . .), and in that time I have attended DragonCon, and been busy on a couple of other fronts that I can't discuss just yet but hope to announce soon. Today, I am happy to point you to an interview I did with Garrett Calcaterra about writing professionally in the current market. Originally, Garrett interviewed me for an article he published at Black Gate Magazine that touched on these issues and focused not only on me, but also on fellow writers M. Todd Gallowglas, Wendy N. Wagner, and Patrick Hester. Today, the full interview is available here, on Garrett's website. I hope you enjoy it.
I'm On Twitter
February 4, 2009
So last night I let some friends (if you can call them that!) talk me into joining Twitter and I can already tell that this was a serious mistake. We're talking major procrastination enabler here. The guilty parties were Alethea Kontis, Mary Robinette Kowal, and Stephen Gould — I offer their names so that my agent and editor will know exactly who to blame for all future missed deadlines.
Anyway, if you're on Twitter and you'd care to follow me, my username (as always) is DavidBCoe. Looking forward to your tweets!


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I still had a massive itch to kill zombies when I finished Dead Nation earlier this year. I already owned Burn Zombie Burn and tried playing it again, but it just didn't quite do the trick. Browsing the Playstation Store, I came across Zombie Apocalypse, a little twin-stick shooter that was published in 2009. I was impressed with the demo, so I bought the game. However, with the deeper gameplay of Dead Nation, I didn't progress very far in Zombie Apocalypse.
Zombie Apocalypse 2: Never Die Alone was released this Fall, and for some reason my curiosity was piqued. I think it was the driving question of "what made them decide to release a sequel to a flat game?" Well, I am happy to say, they learned at least SOMETHING from similar games, and made a much more fleshed out interpretation of what a proper story should be. I'll start out by saying I did not get to play online, as everyone is currently playing Modern Warfare 3, Battlefield 3, and Uncharted 3, so I never could find a game even after looking online multiple times.
The game is designed to be a multiplayer experience and the single player suffers because of it. You have four characters, each with their own back-story and unique skills that they bring to the table. Unfortuntately, the back-stories are played out via static images and narration in the beginning, and are anything but deep.
Jeremy is a mouthy gamer who speaks with slang terms like n00b, "gg", and the like. If this game took itself seriously, this would be a serious problem from the get-go, but I found it mildly entertaining. He has an SMG, the dreaded C4 Teddy Bear, and a quad damage perk. Father Bill is a preacher/religious figure armed with a shotgun, Molotovs, and a healing perk. Alma is the group's sniper who can throw (and detonate) a propane tank into a crowd of zombies, and has an awesome gun turret perk. Last but definitely not least, Def Money has dual pistols, an awesome boom box that makes zombies dance, and a perk that allows you to imperviously rush up with your cricket bat and take zombies out in one hit.
The wide range of options is great for single player, as I shuffled through them clumsily to take advantage of everyone's equipment. But I couldn't imagine being stuck as one character through my playing time with a group. As characters, none of them are enjoyable to play for a long period of time. But you have to use the characters and their various abilities in order to level them up (another new and welcome feature to the sequel). As you progress through the story, your hard-earned cash can be spent to level up five different attributes: Speed, ranged weapons, melee, their zombie bait (C4 Teddy Bear, for example), and their ability. This may require you to replay some levels as different characters in order to progress, as I had to do.
The story is brief and, if you choose to replay a level, you can't skip the cut scenes. Your characters are trying to make it to Def Money's yacht, but they have a few pit stops and problems along the way. New variations of zombies, and bosses will also make appearances to keep things from keeping too monotonous. Each of the ten chapters have five different objectives such as "finish in under 5:00 minutes" or "get x amount of environmental kills"; coupled with leveling up your characters, I really enjoyed going back and trying to complete different objectives. It adds a certain replayability [plus that trophy for maxing out all characters, but I digress] if you feel the need to slaughter more zombies.
My biggest problem with playing the single-player was how clunky the UI seemed. Switching characters is mapped to the L1 and R1 buttons but, to me, it seems like it would have been much easier to map them to the D-pad and have everyone's info laid out on the screen accordingly. Rather than tapping a button twice and hoping it'll land on who you want to control, you could tap up on the pad and have someone selected. Most of the time I died was because I was wresetling with character selection.
While the difficulty seemed rough in the beginning, even after backtracking and leveling up some more, I breezed through the rest of the game once I learned how to work each characters' strength.
While the cast annoyed me pretty much the entire time, I did find myself laughing a few times, most notably at the South Park reference when a zombie moaned "chaaaanggee". The balance between the character abilities and weapons are really the best thing about Zombie Apocalypse 2, and once you learn to use them all efficiently, the game works more in your favor.
After completing the campaign, you unlock Survival mode; both offline and online. There are two variations, Classic and Blackout; Classic is much like the first Zombie Apocalypse game, and Blackout is exactly like Classic only a LOT darker. You face off against waves of zombies, collect cash, and wait for the end of the night. Each wave turns out more zombies, and eventually they start to throw boss zombies at you. When you finally kick the bucket, you'll expect to be rewarded with the money you collected, but nooooo. Instead, you are greeted with a "Game Over" screen. That's it. So, outside of simply wanting to play Survival mode or getting a few trophies, there really isn't much of an incentive to continue playing.
For $9.99, Zombie Apocalypse 2: Never Die Alone is a good fix if you need a new twin-stick shooter to play. While there are better examples on the PSN or XBLA, the strategy ZA2 brings to the table makes it worth playing if it is a genre you enjoy. The story can be annoying whether or not you decide to take it seriously. Don't expect to find random games online, either, as (at least on the PSN), it's like wandering through a town hit with the plague itself.
- twin-stick, arcade action
- diverse characters to play
- annoying character dialogue
- short campaign
- clunky UI
Score: 3*/5


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Click & Collect
Available from our UK stores or myHermes Parcelshops
Product Features
Become a master of Moroccan cookery with Tala's cone-shaped measure. Inside, clearly marked, are the measurements to create the Chermoula Marinade, an essential starting point for a multitude of recipes, as well as a selection of other recipes. The handy recipe booklet included gives clear instructions, making it easy to accomplish a North African feast at home. Comes in a lovely storage tin with a recipe booklet.
14.5cm (5¾") H.
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Product number: 19297


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Singer songwriter Julia Sinclair talks about her process and how her guitar influences her playing.
For tour info and more, please visit http://www.juliasinclair.com/
The Goodnight stopped by LA studio, Beta Records for a Taylor Session and a brief interview.
Thompson Square's Keifer Thompson discusses the challenges he and his wife, Shawna faced while pursuing their dreams of making it as songwriters and performers in Nashville, TN.
For more on Thompson Square, visit http://www.thompsonsquare.com