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N° 21 — Ian Bradley
and the denim jacket"I really love seeing older men wearing denim jackets they've clearly have had for many years."(18 March, 2017) -
N° 20 — Thomas Danthony
and the shoes"I always look sexy whatever I wear."(10 March, 2017) -
N° 19 — Sam McKnight
and the Fred Perry polo shirts"I have at least a hundred Fred Perry polo shirts. There's rarely a day I don't wear one."(28 February, 2017) -
N° 18 — Steven Gregor
and the simple black T-shirt"I don't own a washing machine, so all my clothes are laundered in a nearby launderette. The attendant who washes and folds my clothes is a star."(18 February, 2017) -
N° 17 — Zaldy
and the bottomless shorts drawer"I think athletic wear has really given men the opportunity to confidently, if not shamelessly, display their full peacock."(9 February, 2017) -
N° 16 — Stephen Jones
and the hat"I've always been interested in looking older than I am, and never younger than I am, because I think it gives me an authority."(25 January, 2017) -
N° 15 — Hed Mayner
and the XL white buttoned–down shirts"I went through a period where I bought clothes only in large men's stores where they sell sizing like 3XL, 4XL, 5XL. Everything was huge on me."(18 January, 2017) -
N° 14 — Hayden Thorpe
and the work shirt"It has been a long and often ugly journey to get my wardrobe to the state it's in now."(16 January, 2017) -
N° 13 — Devendra Banhart
and the three scarves"I used to dress up for the grocery store, dress higher up for a party, and dress way down for a show."(1 January, 2017) -
N° 12 — Sam Ashby
and the Blundstone boots"I think winter is the sexiest season. I love stripping down to longjohns and a pair of thick wool socks in front of the fire at home in Yorkshire."(24 December, 2016) -
N° 11 — Johan Kugelberg
and the most unbelievable cotton"ADI DASSLER was best man at my grandfather's wedding."(15 December, 2016) -
N° 10 — Bart de Baets
and the knitted sweater"A good sweater, often an old worn one, can look really quite sexy. Wearing sweaters doesn't make me stand out. I don't expect them to make me do so."(23 November, 2016) -
N° 9 — Jos Gibson
and the denim jeans"It's all about the fit on the waist, the bum and the thigh. It has to be right."(14 November, 2016) -
N° 8 — Micka Agosta
and the lightweight jacket"I tend to wear something until I get bored with it, and just before I go to throw it out, it's suddenly the most important item in my wardrobe. This cycle is vicious."(3 November, 2016) -
N° 7 — Daniel Haworth
and the double-pleated woollen trousers"My room is a mess. It's just piles of clothes. However, I'm good with an iron so it's no problem."(27 October, 2016) -
N° 6 — Nick Wooster
and the blue oxford shirt"Whenever I see a blue oxford shirt I still get that feeling. It just seems correct. And by that I mean it always looks good."(20 October, 2016) -
N° 5 — Dmitry Komis
and the bow tie"I feel like men my age and younger think dressing is a chore. I definitely feel more comfortable in black tie than in gym clothes."(13 October, 2016) -
N° 4 — Bonne Reijn
and the Bonne Suit"I'm constantly caught between looking like a bum and trying to look like the late PRINCE BERNHARD of the Netherlands."(6 October, 2016) -
N° 3 — Edwin Dorley
and the long-sleeved T-shirt"I operate on a one-in, one-out basis: usually when a shirt becomes too irreversibly dirty it's time to move on."(29 September, 2016) -
N° 2 — Angelo Flaccavento
and the blue blazer"Blue is the quintessential masculine colour: deep but not stark, academic yet easy going."(22 September, 2016) -
N° 1 — Alex Tieghi-Walker
and the patterned shirt"There are photos of me as a kid wearing tie-dye T-shirts
and African prints. The look stuck!"(16 September, 2016)
An ongoing series of in-depth conversations about men's very personal clothing habits.


text:
Jay Heidner
Live in Berkeley, CA
8 pets - 2 dogs, 2 cats, 2 birds, 2 lizards
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Going through a divorce is a very mentally taxing thing. Couple with that the several legal proceedings, and the whole affair becomes nothing short of hell. Now, there are some people who think that getting a lawyer involved may make things more complicated, because some lawyers may cheat them. Any and every person going through a divorce needs not just a lawyer, but a great one because that is when things will become easier for you.You need to be well versed with all the responsibilities, duties and rights you have according to the law. And only a lawyer will be able to give you that training and inputs. If you don't know your rights properly, you won't be able to figure the amount of financial assets you are entitled to, the chance you have for securing custody for your child and any and everything that is even remotely related to your divorce.While you may turn to that one friend who recently had a divorce for advice, you should remember that what your friend will be able to help you with will be limited to what she had experience with during her divorce proceedings, unless off course she is herself a lawyer. Every divorce case is different because of several things, so just because someone else went through a divorce doesn't mean they will be able to help you out during yours too.
There are issues which crop up after a divorce is filed. If you don't have a lawyer, you probably won't even be aware of them and ultimately, you will end up losing the case. You may be having an amicable divorce, but you never know what your partner may be up to. So, it's always better to turn to a practising divorce lawyer for advice at times like these.
The San Antonio divorce lawyers excel at being by your side during any kind of divorce, and they have a great track record. Check out their website to find out more about them.


text: Everything's Ring-a-Ding in Fallout: New VegasOwen Good6/12/10 12:00pmFiled to: Clipsfallout new vegasE3E32010Bethesda SoftworksBethesdaFallout522EditPromoteShare to KinjaToggle Conversation toolsGo to permalink Fallout 3 introduced us to the Ink Spots and 1941's "I Don't Want to Set the World on Fire." Fallout: New Vegas opens here with Kay Kyser's. "Jingle Jangle Jingle," the No. 1 hit for eight weeks in 1942. Advertisement That's about the only useful thing I can add to this two-minute video, slam full of gameplay, heralding New Vegas's appearance at E3 2010. It speaks for itself, and I advise you to listen. And watch.Reply522 repliesLeave a reply


text:
Publication number | US1209374 A |
Publication type | Grant |
Publication date | Dec 19, 1916 |
Filing date | May 26, 1916 |
Priority date | May 26, 1916 |
Publication number | US 1209374 A, US 1209374A, US-A-1209374, US1209374 A, US1209374A |
Inventors | Angelo Andreolli |
Original Assignee | Angelo Andreolli |
Export Citation | BiBTeX, EndNote, RefMan |
Referenced by (8), Classifications (8) | |
External Links: USPTO, USPTO Assignment, Espacenet | |
APPLICATION FILED MAY 26, l9l6.
1 QQQfiY, Patented. Dec. 19, 1916.
WITNESSES 76 l/Vl/E/VTOB 7.9 away/7112755471111:
fim f 87 7 W3 ran sra'rns aren't role.
' Application filed ma 26, 1916. Serial No. 99,955.
To all whom it may concern:
Be it known that I, ANGELO ANDREOLLI, a citizen of the United States, and a resident of Meadow Lands, in the county of Washington and State of Pennsylvania, have invented new and useful Improvements in Hose-Coup1ings', of which the following is a specification.
My invention is an improvement in hose couplings, and has for its object to providea coupling of the character specified, wherein the coupling members are connected together without the use of screw threads or the like, and wherein the pressure of the water passing through the coupling assists in maintaming the connect1on water tight, and wherein the members are easily connected and disconnected.
In the drawings, Figure 1 is a perspective View of the improved coupling, Fig. 2 is a longitudinal section of the same, Fig. 3 is a section on the line 33 of Fig. 2, looking in the direction of the arrowsadjacent to the line, and Fig. 4 is a perspectlve view of the yoke. Y
In the present embodlment of the invention the hose sections 1 and 2 which areto be connected, are fitted over the reduced tit) ends 3 and 7 of the sections of a rigid coupling, the said ends being roughened or corrugated externally to prevent slipping of the hose sections.
The hose section 1 is secured to the reduced end 3 of the coupling section 5, by means of a band 4v which encircles the hose-section and clamps it to the reduced end of the coupling section. The section 2 is secured to the reduced portion 7 of the other coupling section 11, by means of a similar band 6, and the coupling section 5 is internally enlarged as indicated at 9 to receive the section 11.
An annular shoulder 8 is formed at the inner end of the enlarged portion, and the end of the section 11 abuts against the shoulder. An external shoulder 10 is formed on the section 11 at the end of the other coupling section, and a gasket 22 is arranged .between this shoulder and the end of the section.
The coupling section 11 is provided with an external annular groove or'reoess 12 near its inner end, and a gasket or packing ring 13-is seated in this recess. A series of openings 14 is prorided in the bottom of this recess 12, the said openings permitting the Specification of Letters Patent.
Patented Dec.- 19, 1916.
pressure of the liquid in-the hose section to be communicated to the gasket or packing ring, for pressing'the ring outward into close contact with the interior of the section 5.
Lugs 15 are provided on opposite sides of the section 5, and arms 16 are pivoted to the lugs by means oi pins or screws 17. A yoke 18 is connected to the arms at the ends remote fromthe pins 17, and this yoke is adapted to embrace the coupling section 11. The said section 11 has lugs 19, which are adapted to be engaged by the ends of the yoke which extend outwardly beyond the arms, to prevent longitudinal movement of the sections away from each other.
When the yoke is moved in the direction of the arrow indicated at A- in Fig. 1, the coupling sections 5 and 11 will be forced together, thus placing the gasket or washer 22 under compression. The yoke 18 is provided with a cam member or lever 23 pivoted to the yoke,.and having a handle 24:, and when the yoke has been moved in the direction of the arrow A. to the pointwhere the cam member 23 will engage behind the shoulder 24, between the body of the coupling 11 and the reduced portion 7, the sections' 3 and 5 of the coupling will be held in locked relation, The cam member 23 when locked prevents the outward movement of the yoke 18 away from the section 2. This cam member locks behindthe shoulder 2% which is formed between the body of the section and the reduced portion 7 over which the hose section slips.
As shown in Fig. 1,'the reduced portion 0 the section 5 is provided with a recess 25, and the section 11 of the coupling is provided with a lug 26 which is adapted to engage the recess to insure that the sections retain their proper relative annular position with respect to each other.
In use, when the coupling sections are connected, the pressure of the water passing through the couplings, forces the flexible gasket 13 outward into close engagement with the inner wall of the enlargement 9 of the section 5, thus preventing escape water through the contracting surface of the coupling section.
To uncouple the sections of the hose, it is only necessary to swing the cam 23 until the nose thereof disengages from behind the shoulder 24. to permit the yoke 18 to swing t'ween'the sections, oppositely arranged linkpivoted to the first-named section, a yoke pivoted to "the ends of the links and shaped to fit and adapted to engage around the other section, said section having lugs for engagement by the yoke, and a cam lever pivoted to the yoke and adapted to engage behind the shoulder formed between the body of the section and the reduced portion.
Citing Patent | Filing date | Publication date | Applicant | Title |
US2677560 * | Mar 25, 1950 | May 4, 1954 | Wade & Co R M | Sealing and self-draining means for pipe couplers |
US3210012 * | Sep 23, 1963 | Oct 5, 1965 | Peter Caird | Spray nozzle for fire hose and the like |
US4068870 * | Apr 2, 1976 | Jan 17, 1978 | Gaymar Industries Incorporated | Flexible hose coupling |
US4932114 * | Jul 14, 1988 | Jun 12, 1990 | North American Instrument Corp. | Method of making a rotating adapter for catheters |
US5078433 * | Apr 12, 1990 | Jan 7, 1992 | North American Instrument Corp. | Rotating adapter for catheters and the like |
US5112085 * | Nov 16, 1990 | May 12, 1992 | Ford Motor Company | Tube coupling with combination retainer and disassembly tool |
US5248306 * | Aug 25, 1992 | Sep 28, 1993 | Abbott Laboratories | Axial retainer for flow connectors |
US6176523 * | Mar 4, 1998 | Jan 23, 2001 | The Lamson & Sessions Co. | Joint for variable wall thickness conduit |
U.S. Classification | 285/88, 285/910, 285/374, 285/320, 285/106, 285/313 |
Cooperative Classification | Y10S285/91, F16L37/252 |


text: Southern Utah Desert Spines splitboard.com November 20, 2011 Videos The 2009-2010 winter was not an average winter for Utah. The mountains in the north were plagued with sporadic snowfalls and dangerous avalanche conditions, while the southern regions were reaping the benefits of a strong el nino pattern. Neil and I had wanted to explore the ski terrain in southern utah for many years, but we knew we had to wait for the right snow year. Early last winter, we made a few recon missions and found the kind of terrain we were looking for. Truly beautiful and inspiring ski terrain that none of us had ever seen before. Finally in late March, myself, Neil, and Josh Finbow made one more attempt at skiing and filming some lines before the strong desert sun melted them out for good. A combination of good timing and preparation, along with a little luck, led to some of the wildest riding we had ever encountered. The whole time we felt so lucky to be skiing this exotic, weird terrain under such good conditions. It really seemed like a once in a lifetime opportunity so we treated it as such. Utah's color country might provide the perfect postcard image for millions of tourists, but when it's covered in snow, its the skiers and boarders who can become inspired by the incredible landscape and find new ways to enjoy it. Words by Ian Provo. Here are some photos Ian and I took on our trip…Snowboard photos taken by Ian and Skiing photos taken by Myself and Josh Finbow. The big line, in all her glory! One of the best runs of my life… the next day, BOOOM- broken legs… Leave a Reply Cancel Reply Your email address will not be published.CommentName* Email* Website Notify me of follow-up comments by email. Notify me of new posts by email.


text:
Islam, Orientalism and Intellectual History: Modernity and the Politics of Exclusion since Ibn Khaldun. Mohammad Salama. London: I.B. Tauris, 2013. ISBN: 9781780764504 This book examines the relationship between Islam and the West from the point of view of intellectual history during the Enlightenment, colonialism, and the postcolonial era. As Salama read more »
Loci Sacri: Understanding Sacred Places. T.Coomans, H. De Dijn, J. De Maeyer, R. Heynickx, and B. Verschaffel (eds.) Leuven: Leuven University Press, 2012. ISBN: 9789058678423 As a historian of Africa and as a teacher of world history, I am very intrigued by the subject of sacred spaces as a way read more »
A Short History of Film: Second Edition. Wheeler Winston Dixon and Gwendolyn Audrey Foster. New Brunswick, New Jersey: Rutgers University Press, 2013. ISBN: 9780813560557 Wheeler Winston Dixon and Gwendolyn Audrey Foster's A Short History of Film provides an excellent starting point into the study of film history. The work read more »
Imagic Moments: Indigenous North American Film. Lee Schweninger. London: The University of Georgia Press, 2013. ISBN: 9780820345154 Lee Schweninger's book Imagic Moments: Indigenous North American Film is the latest text to examine the cinematic achievements of Aboriginal directors and actors in the rich and increasingly popular field of North American read more »
Gender and the Negotiation of Daily Life in Mexico, 1750-1856. Sonya Lipsett-Rivera. Lincoln: University of Nebraska Press, 2012. ISBN: 9780803238336 Sonya Lipsett-Rivera opens Gender and the Negotiation of Daily Life in Mexico with the assertion that "on a day-to-day basis the aspects of life that most preoccupied people were not read more »
The Feedback Loop: Historians Talk about the Links between Research and Teaching. Antoinette M. Burton, et. al. eds. Washington, D.C: The American Historical Association, 2013. ISBN: 9780872292011 Explaining what I do as a historian is often a difficult task. Much of society -- many of my students included – assume read more »
Inka Human Sacrifice and Mountain Worship: Strategies for Empire Unification. Thomas Besom. Albuquerque: University of New Mexico Press, 2013. ISBN: 9780826353078 The consolidation of the Inka Empire in only 90 years, for Thomas Besom, is one of the most exceptional events in South American history. Most scholars in the field, read more »
Excavating Modernity: The Roman Past in Fascist Italy. Joshua Arthurs. New York: Cornell University Press, 2012. ISBN: 9780801449987 Throughout contemporary academic spheres, recent discourse revolving around the history of Fascism has turned towards the regime's vision of modernism. According to Excavating Modernity, the implication of said scholarly approach necessitates read more »
Column: Partnership in the Japanese and American Imaginary: Gender and the Mediation of Difference in Hayao Miyazaki's and Walt Disney Studio's Animated Movies Abstract This paper compares two animated movies made by Hayao Miyazaki, Nausicaa of the Valley of the Wind (1984) and Princess Mononoke (1997) and two movies made read more »
Mark My Words: Native Women Mapping Our Nations. Mishuana Goeman. Minneapolis: University of Minnesota Press, 2013. ISBN: 9780816677900 Mishuana Goeman's Mark My Words: Native Women Mapping Our Nations challenges readers to think about how indigenous North American women's writing creates maps and spaces that complement and counter colonial, patriarchal, conventionally-considered read more »
Published by the Midwest World History Association (MWWHA), housed at The College of St. Scholastica. See also, The Middle Ground's curated Facebook Page.


text:
Kerberos Productions: Liir
Lindsey Wakefield -- Friday, March 15, 2013 at 12:01 PM
Hey everyone! Here are a few more illustrations that I did for Kerberos Productions. This time I did the Liir, a highly intelligent cetacean race that communicate via the tentacles on their face. These guys have a lot of back story, but I just stuck with the basics.
Thanks for looking and I hope everyone enjoys!


text:
Related Sections: | Scotland | Bon Homme County |
Scotland Golf Club
1031 Stafford StScotland, SD 57059
Phone: (605)583-4244
This course has a creek that comes into play on all but two holes. There are many recently planted trees, yet more of the old trees will affect your shots. The terrain is slightly hilly, so expect some uneven lies.
Holes: 9 | Greens: Bent Grass | Fairways: Bluegrass |
Greens Fees: | $20 or Less |
Course Type: | semi-private |
Dress Code: | No dress code |
Season: | May 1 to Oct 15 |
Guest Policy: |
* Update this Course: Update/modify the details for this golf course's listing.
*All Greens Fees provided are subject to change. Please call the golf course to confirm the current fees.


text:
Quantitative trait locus mapping of genes associated with vacuolation in the adrenal X-zone of the DDD/Sgn inbred mouse
- Jun-ichi Suto1Email author
© Suto; licensee BioMed Central Ltd. 2012
Received: 20 June 2012
Accepted: 1 November 2012
Published: 6 November 2012
Adrenal gland of mice contains a transient zone between the adrenal cortex and the adrenal medulla: the X-zone. There are clear strain differences in terms of X-zone morphology. Nulliparous females of the inbred mouse DDD strain develop adrenal X-zones containing exclusively vacuolated cells, whereas females of the inbred mouse B6 strain develop X-zones containing only non-vacuolated cells. The X-zone vacuolation is a physiologic process associated with the X-zone degeneration and is tightly regulated by genetic factors. Identification of the genetic factors controlling such strain differences should help analyze the X-zone function. In this study, a quantitative trait locus (QTL) analysis for the extent of X-zone vacuolation was performed for two types of F2 female mice: F2A y mice (F2 mice with the A y allele) and F2 non-A y mice (F2 mice without the A y allele). These were produced by crossing B6 females and DDD.Cg-A y males. DDD.Cg-A y is a congenic mouse strain for the A y allele at the agouti locus and is used for this study because a close association between the X-zone morphology and the agouti locus genotype has been suggested. The A y allele is dominant and homozygous lethal; therefore, living A y mice are invariably heterozygotes.
Single QTL scans identified significant QTLs on chromosomes 1, 2, 6, and X for F2 non-A y mice, and on chromosomes 2, 6, and 12 for F2A y mice. The QTL on chromosome 2 was considered to be because of the agouti locus, which has been suggested to be associated with X-zone vacuolation. A significant QTL that interacted with the agouti locus was identified on chromosome 8.
The extent of X-zone vacuolation in DDD females was controlled by multiple genes with complex interactions. The murine X-zone is considered analogous structure to the human fetal zone. Therefore, the results of this study will aid in understanding function of not only of the X-zone but also of the human fetal zone. Identifying the genes responsible for the QTLs will be essential for understanding the molecular basis of X-zone function, which is currently unclear.
Adrenal X-zone DDD mice Quantitative trait locus (QTL) mappingBackground
The mammalian adrenal cortex consists of three separate layers: zona glomerulosa, zona fasciculate, and zona reticularis. These layers have distinct endocrine roles. In rodents, an additional fourth, transient zone is present between the adrenal cortex and the adrenal medulla. In 1924, Masui and Tamura[1] first discovered a presence of a large transient zone at the innermost region of the adrenal cortex that could not be found anywhere else in the adult male cortex. This zone was later named the X-zone by Howard-Miller[2]. A close relationship was observed between X-zone morphology and gonadal functions. For example, the X-zone degenerates at puberty in males and at the first pregnancy in females.
There are apparent sex and strain differences in the morphology of the X-zone. With regard to sex difference, X-zone degeneration occurs earlier in males than in females; the X-zone disappears before 5 weeks of age in males, but gradually decreases in thickness with age in virgin females[3]. With regard to strain differences, inbred mouse strains can be classified into two categories based on the incidence and size of X-zones and the timing of X-zone vacuolation.
Nulliparous females of the inbred mouse DDD strain have thicker X-zones than the C57BL/6J females[3]. In DDD females, the X-zone degenerates with the accumulation of lipids; hereafter, this state is called a "vacuolated X-zone" after the designation given by Tanaka and Matsuzawa[3]. In contrast, in C57BL/6J females, the X-zone degenerates without the accumulation of lipids; hereafter, referred to as a "non-vacuolated X-zone" Strain differences are also observed with regard to the timing of X-zone degeneration. For example, fatty degeneration of the X-zone takes place earlier in A/Cam than in CBA/FaCam females[4].
Such clear sex and strain differences in the X-zone appearance suggest that the morphology of this zone is controlled both hormonally and genetically. Indeed, Shire and Spickett[4] reported that strain differences were a consequence of genetic variations at a single locus, "earlier X-zone degeneration locus" (Ex), on chromosome 7. Tanaka et al.[5, 6] reported a close association between X-zone morphology and the agouti locus genotype on chromosome 2. They suggested that the a allele suppressed the vacuolation of the cells in the X-zone in a dominant manner. While such investigations have been useful, we propose that a genome-wide study would be a more beneficial approach.
Unfortunately, only one study has attempted a genome-wide search for genes controlling X-zone morphology. Di Curzio and Goldowitz[7] recently performed a QTL analysis on adrenal gland weight and structure using BXD recombinant inbred strains, and identified multiple significant and suggestive QTLs. In particular, they identified significant QTLs for X-zone width on chromosome 10 (Mxzwdq1) and 14 (Mxzwdq2) in males.
We have established a novel congenic strain for the A y allele at the agouti locus (chromosome 2) in the inbred DDD/Sgn strain: DDD.Cg-A y . In normal mice, the agouti gene is expressed only in the skin[9, 10], and it regulates pigmentation by serving as an inverse agonist of the melanocortin 1 receptor (MC1R)[11, 12]. However, in A y mice, the A y allele is associated with a large deletion, causing agouti gene expression to be aberrantly controlled by the unrelated Raly gene promoter and leading to its ectopic overexpression[10, 13–15]. As a result, A y mice have a yellow coat. This makes it possible to distinguish the A y mice from non-A y mice visually. In addition, the A y allele is homozygous lethal; therefore living A y mice are invariably heterozygotes. DDD.Cg-A y strain was used for this study because a close association between the X-zone morphology and the agouti locus genotype has been suggested[5, 6]. While Tanaka et al.[5, 6] investigated the effect of the a, A, and A w alleles on the X-zone vacuolation, they did not investigate the effect of the A y allele. Comparison of X-zone morphology between B6 and B6.Cg-A y , and DDD and DDD.Cg-A y makes it possible to reveal the effect of the A y allele. Furthermore, use of DDD.Cg-A y strain together with C57BL/6J strain makes it possible to include the effect of the A y allele in combination with the a and A alleles in the QTL analyses in F2 mice. The objectives of this study were to determine the genetic basis for strain differences in the extent of X-zone vacuolation between the DDD/Sgn and C57BL/6J strains and to clarify a possible effect of the agouti locus genotype on X-zone vacuolation.
The inbred mouse DDD/Sgn strain (hereafter designated DDD, coat color genotype is AABBcc) and the congenic mouse DDD.Cg-A y strain (hereafter designated DDD-A y , A y ABBCC) were maintained at the National Institute of Agrobiological Sciences (NIAS). The inbred mouse C57BL/6J strain (hereafter designated B6, aaBBCC) was purchased from Clea Japan (Clea Japan, Inc., Tokyo, Japan). The congenic mouse B6.Cg-A y strain (hereafter B6-A y , A y aBBCC) was purchased from The Jackson Laboratory (Bar Harbor, ME, USA). The DDD-A y strain was established by introgressing the A y allele from the B6-A y strain in the DDD strain by backcrossing for 12 generations[16]. Hereafter, both DDD-A y and B6-A y are referred to as "A y mice" Similarly, their control littermates, DDD and B6, are both referred to as "non-A y mice" The A y allele at the agouti locus (chromosome 2) is dominant and homozygous lethal; therefore, living A y mice are invariably heterozygotes. A y mice can be distinguished visually by their yellow coat color from non-A y mice.
DDD-A y males were crossed with B6 females to produce the F1 generation, and F1A y (A y aBBCC) mice were intercrossed with F1 non-A y (AaBBCC) mice to produce the F2 generation. F2 females were weaned at 4 weeks. The mice were housed in groups of 4–5 for 16 weeks.
All mice were maintained in a specific-pathogen-free facility with a regular light–dark cycle (12 h light and 12 h dark) and controlled temperature (23 ± 1°C) and humidity (50%). Food (CRF-1; Oriental Yeast Co., Ltd., Tokyo, Japan) and water were freely available throughout the experimental period. All animal experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee of NIAS.
At the age of 16 weeks, mice were euthanized with an overdose of ether and their adrenal glands were removed. Formalin-fixed tissues were embedded in paraffin, sectioned, and stained with hematoxylin and eosin.
"Grade 0" indicates that the X-zone contains only non-vacuolated cells; this corresponds to X-zone morphology in the parental B6 (−A y ) strain. The layer of vacuolated cells in an X-zone of "Grade 3" is the thickest among the grades; this corresponds to X-zone morphology in the parental DDD (−A y ) strain. The layer of vacuolated cells in an X-zone of "Grade 1" and "Grade 2" is thinner than that of "Grade 3" The layer of vacuolated cells in an X-zone of "Grade 2" is apparently thicker than that in an X-zone of "Grade 1".
Alternatively, the occurrence of a vacuolated X-zone was evaluated qualitatively by regarding this as a binary trait. That is, an X-zone of "Grade 0" as indicated above was recognized as containing non-vacuolated cells only and X-zones of "Grades 1–3" were recognized as containing vacuolated cells.
In this study, the grade of X-zone vacuolation was treated in three different ways: as a parametric trait, as a non-parametric trait, and as a binary trait. QTLs that were identified as significant in at least one of these three analyses were considered to be significant.
Genotyping and QTL analysis
Genomic DNA was isolated from the tails of mice using a commercial DNA extraction kit (Wizard Genomic DNA Purification Kit; Promega, Madison, WI, USA). Microsatellite sequence length polymorphisms were identified after PCR amplification of genomic DNA. PCR products were separated by 10% polyacrylamide gel electrophoresis and visualized with ethidium bromide straining.
Mice were genotyped for the following microsatellite marker loci: D1Mit231, D1Mit303, D1Mit10, D1Mit102, D1Mit16, Apoa2 (see below), D1Mit291, D2Mit312, D2Mit296, D2Mit92, D2Mit304, D2Mit285, D2Mit346, D3Mit203, D3Mit25, D3Mit212, D4Mit1, D4Mit178, D4Mit166, D4Mit234, D5Mit267, D5Mit113, D5Mit239, D5Mit161, D5Mit221, D6Mit116, D6Mit224, D6Mit188, D6Mit39, D6Mit108, D6Mit256, D6Mit259, D7Mit250, D7Mit362, D8Mit191, D8Mit205, D8Mit249, D8Mit183, D9Mit59, D9Mit191, D9Mit207, D9Mit198, D9Mit212, D10Mit188, D10Mit183, D10Mit42, D10Mit95, D11Mit236, D11Mit36, D11Mit124, D11Mit61, D12Mit136, D12Mit172, D12Mit156, D12Mit259, D12Mit141, D12Nds2, D13Mit207, D13Mit64, D13Mit110, D13Mit213, D13Mit171, D14Mit64, D14Mit193, D14Mit165, D15Mit174, D15Mit184, D15Mit193, D16Mit131, D16Mit57, D16Mit136, D16Mit139, D16Mit49, D17Mit164, D17Mit176, D17Mit139, D17Mit93, D17Mit123, D18Mit21, D18Mit149, D18Mit152, D18Mit25, D19Mit32, D19Mit91, D19Mit35, DXMit166, DXMit119, DXMit64, and DXMit38. Apoa2 (apolipoprotein A-II) was genotyped with a PCR-RFLP method according to the Suto et al.[18] procedure.
It should be noted that the chromosome 7 is divided into two parts. As a consequence of the introgression of the Tyr locus from the B6 strain, a mid-part of the DDD genome on chromosome 7 is replaced by a B6 genome in DDD-A y mice. In this study, a region proximal to the B6 region was defined as "chromosome 7.1 (D7Mit250)" while a region distal to the B6 region was defined as "chromosome 7.2 (D7Mit362)".
Of a total of 298 F2 females, 148 were F2 non-A y and 150 were F2A y mice. QTL analysis was done using R/qtl[19, 20]. First, F2A y and F2 non-A y mice were analyzed independently. Threshold LOD scores for suggestive (P < 0.63) and significant (P < 0.05) linkages were determined by performing 1,000 permutations for each trait. For significant QTLs, a 95% confidence interval (CI) was defined by a decline of 1.5-LOD. After these single QTL scans, pair-wise evaluations for potential interactions between loci were made. At this stage, threshold LOD scores were based strictly on those recommended by Broman in "A brief tour of R/qtl" (http://www.rqtl.org). Next, data for F2 non-A y and F2A y mice were combined and reanalyzed. The calculations for the threshold LOD scores were repeated. The presence or absence of possible statistical interactions between the genotypes at QTLs and the agouti locus (non-A y and A y ) was evaluated by two-way ANOVA.
The combined effects of all QTLs, including those that were significant and suggestive, were assessed using multiple QTL models. The percent of the variance explained by each QTL was reported based on these models.
Statistical analysis between two groups was performed using the Student's or Welch's t-test, or the Wilcoxon or Kruskal-Wallis test. Statistical analysis between more than two groups was performed using Tukey's multiple comparisons tests with JMP8 software (SAS Institute Japan, Tokyo, Japan). P <0.05 was considered to be statistically significant.
Comparisons of X-zone morphologies among the DDD-A y , DDD, B6-A y , and B6 strains
At the age of 16 weeks, X-zone morphologies in both sexes were compared among the DDD-A y , DDD, B6-A y , and B6 strains. Comparison of X-zone morphology between B6 and B6.Cg-A y , and DDD and DDD.Cg-A y makes it possible to reveal the effect of the A y allele. In agreement with previous findings, the X-zone was absent in males, irrespective of strain or agouti locus genotype (Figure1A). Females had X-zones and as expected, there were gross strain differences in the extent of vacuolation. DDD-A y and DDD females had X-zones that contained exclusively vacuolated cells, whereas B6-A y and B6 females had X-zones containing only non-vacuolated cells (Figure1B). X-zone vacuolation in all DDD-A y (n = 4) and DDD (n = 2) females was scored as grade 3 (Figure2A). In contrast, X-zone vacuolation in all B6-A y (n = 4) and B6 (n = 2) females was scored as grade 0.
X-zone vacuolation in F1 and F2 mice
X-zone vacuolation was also evaluated in (♀B6 × ♂DDD-A y ) F1 and F2 mice. Because the agouti locus genotype of B6 strain was a/a and that of DDD-A y strain was A y /A, the agouti locus genotype of F1 mice was either A/a (F1 non-A y mice) or A y /a (F1A y mice). In the similar way, F2 non-A y mice had either A/a or a/a genotype and F2A y mice had either A y /a or A y /A genotype (See also Materials and methods). All F1 non-A y (n = 5) and all F1A y (n = 5) mice had vacuolated X-zones of grade 1 or 2; the average grade was 1.6 in both F1 types.
Histograms showing these grade distributions in F2 non-A y (n = 148) and F2A y (n = 150) mice are shown in Figure2B. The mean ± S.E. grades in F2 non-A y and F2A y mice were 1.92 ± 0.09 and 2.05 ± 0.09, respectively. There was no significant difference between these two F2 types. When the grade was treated as a binary trait, the number of mice with grade 0 (absence of vacuolation) was 16 (10.8%) in F2 non-A y and 24 (16.0%) in F2A y mice.
QTL mapping
QTLs identified for F 2 non-A y mice by single QTL scans
Method | Chromosome | Location (cM)a | 95% CI (cM)b | Max LODc | Nearest marker | High alleled | Namee |
parametric | 1 | 40 | 17–69 | 3.40 * | D1Mit10 | DDD | Xzvdq1 |
2 | 73 | 65–90 | 5.26 * | D2Mit285 | DDD | Xzvdq2 | |
4 | 24 | 7–72 | 2.32 | D4Mit178 | DDD | ||
6 | 67 | 49–75 | 3.01 | D6Mit256 | DDD | ||
8 | 21 | 21–41 | 2.22 | D8Mit191 | DDD | ||
X | 51 | 32–57 | 3.72 * | DXMit64 | B6 | Xzvdq3 | |
non-parametric | 1 | 39 | 14–68 | 3.20 * | D1Mit303 | DDD | Xzvdq1 |
2 | 76 | 68–87 | 6.24 * | D2Mit285 | DDD | Xzvdq2 | |
4 | 25 | 7–75 | 2.17 | D4Mit178 | DDD | ||
6 | 67 | 50–75 | 3.26 * | D6Mit256 | DDD | Xzvdq4 | |
8 | 24 | 21–43 | 2.13 | D8Mit191 | DDD | ||
X | 43 | 30–57 | 3.07 | DXMit64 | B6 | ||
binary | 1 | 65 | 20–89 | 2.41 | D1Mit102 | DDD | |
2 | 50 | 2–69 | 2.14 | D2Mit92 | DDD | ||
X | 50 | 39–57 | 3.80 * | DXMit64 | B6 | Xzvdq3 |
QTLs identified for F 2 A y mice by single QTL scans
Method | Chromosome | Location (cM)a | 95% CI (cM)b | Max LODc | Nearest marker | High alleled | Namee |
parametric | 2 | 87 | 3–101 | 1.99 | D2Mit285 | DDD | |
6 | 60 | 55–70 | 8.95 * | D6Mit256 | DDD | Xzvdq4 | |
12 | 52 | 22–62 | 3.42 * | D12Mit259 | B6 | Xzvdq5 | |
non-parametric | 2 | 87 | 72–101 | 3.40 * | D2Mit285 | DDD | Xzvdq2 |
6 | 65 | 53–73 | 7.71 * | D6Mit256 | DDD | Xzvdq4 | |
12 | 52 | 13–62 | 2.63 | D12Mit259 | B6 | ||
binary | 6 | 59 | 37–71 | 4.36 * | D6Mit256 | DDD | Xzvdq4 |
12 | 39 | 13–62 | 2.60 | D12Mit156 | B6 | ||
18 | 9 | 9–32 | 2.64 | D18Mit21 | DDD |
QTLs identified for combined F 2 (F 2 non-A y plus F 2 A y ) mice by single QTL scans
Method | Chromosome | Location (cM)a | 95% CI (cM)b | Max LODc | Nearest marker | High alleled | Namee |
parametric | 1 | 34 | 20–58 | 3.95 * | D1Mit303 | DDD | Xzvdq1 |
2 | 85 | 64–98 | 5.03 * | D2Mit285 | DDD | Xzvdq2 | |
4 | 22 | 7–48 | 2.16 | D4Mit178 | DDD | ||
6 | 65 | 56–72 | 10.90 * | D6Mit256 | DDD | Xzvdq4 | |
12 | 54 | 13–62 | 2.50 | D12Mit141 | B6 | ||
non-parametric | 1 | 35 | 17–59 | 3.55 * | D1Mit303 | DDD | Xzvdq1 |
2 | 86 | 67–96 | 6.54 * | D2Mit285 | DDD | Xzvdq2 | |
4 | 22 | 7–55 | 2.14 | D4Mit178 | DDD | ||
6 | 66 | 55–73 | 10.58 * | D6Mit256 | DDD | Xzvdq4 | |
binary | 1 | 55 | 18–71 | 2.82 | D1Mit102 | DDD | |
6 | 60 | 37–75 | 3.40 | D6Mit256 | DDD | ||
12 | 54 | 13–62 | 2.91 | D12Mit141 | B6 |
Summary of single QTL scans for combined F 2 mice using the agouti genotypes as covariates
Chromosome | LOD scores[peak position (cM),name] | ||
agouti as an additive covariate (LODa)a | agouti as an interactive covariate (LODf)b | LODi(LODf-LODa)c | |
1 | 4.03 (36, Xzvdq1) | ||
2 | 5.42 (80, Xzvdq2) | 6.03 (82, Xzvdq2) | |
6 | 11.22 (65, Xzvdq4) | 11.89 (65, Xzvdq4) | |
8 | 4.52 (24, Xzvdq6) | 3.84 (23, Xzvdq6) |
Next, possible interactions were analyzed pair-wise for F2 non-A y and F2A y mice. No significant pair-wise interactions were identified.
Multiple QTL analyses
F2type | Chr@cMa | dfb | Type III SSc | LOD | %varianced | F value | P value |
non-A y | firstname.lastname@example.com | 2 | 10.167 | 3.54 | 6.64 | 7.91 | 0.00056 |
firstname.lastname@example.com | 2 | 10.15 | 3.53 | 6.64 | 7.90 | 0.00057 | |
firstname.lastname@example.com | 2 | 5.76 | 2.05 | 3.76 | 4.48 | 0.013 | |
email@example.com | 2 | 10.42 | 3.62 | 6.81 | 8.11 | 0.00047 | |
email@example.com | 2 | 3.65 | 1.32 | 2.39 | 2.84 | 0.062 | |
A y | email@example.com | 2 | 4.53 | 1.15 | 2.41 | 2.56 | 0.081 |
email@example.com | 2 | 37.34 | 8.43 | 19.91 | 21.13 | 9.13 × 10−9 | |
firstname.lastname@example.com | 2 | 11.48 | 2.83 | 6.12 | 6.50 | 0.0020 | |
combined | firstname.lastname@example.com | 2 | 15.58 | 4.37 | 4.56 | 9.83 | 7.51 × 10−5 |
email@example.com | 6 | 28.61 | 7.82 | 8.37 | 6.01 | 6.22 × 10−6 | |
email@example.com | 2 | 11.49 | 3.25 | 3.36 | 7.25 | 0.00085 | |
firstname.lastname@example.com | 2 | 43.41 | 11.52 | 12.70 | 27.37 | 1.38 × 10−11 | |
email@example.com | 6 | 17.58 | 4.91 | 5.14 | 3.69 | 0.0015 | |
firstname.lastname@example.com | 2 | 6.23 | 1.79 | 1.82 | 3.93 | 0.021 | |
firstname.lastname@example.com:email@example.com | 4 | 13.71 | 3.86 | 4.01 | 4.32 | 0.0021 |
The suggestive effect of the agouti locus genotype
Effect of agouti locus genotypes on the extent of X-zone vacuolation
F2type | Trait | Mean grade (rank score) by agouti locus genotypea | Nominal P value | |
A/a(n=78) | a/a(n=70) | |||
F2 non-A y | parametric | 2.28 ± 0.11 | 1.51 ± 0.11 | 2.33 × 10−6 |
non-parametric | 89.74 | 57.51 | 9.64 × 10−7 | |
binary | 0.92 ± 0.03 | 0.86 ± 0.04 | 0.21 | |
A y /A (n = 80) | A y /a (n = 70) | |||
F2A y | parametric | 2.24 ± 0.12 | 1.84 ± 0.13 | 0.031 |
non-parametric | 83.51 | 66.34 | 0.00048 | |
binary | 0.86 ± 0.04 | 0.81 ± 0.05 | 0.43 |
Multigenic basis responsible for X-zone vacuolation
The extent of X-zone vacuolation in F1 mice was intermediate compared with the parental strains, and that in F2 mice was varied. Therefore, the extent of X-zone vacuolation is suggested to be a quantitative phenotype that is determined by multiple genes. Indeed, results of our QTL analyses revealed a multigenic basis for X-zone vacuolation.
Among the identified QTLs, Xzvdq2 and Xzvdq4 on chromosomes 2 and 6, respectively, were identified in both F2 types; the LOD scores for these QTLs increased in the combined F2 mice. Therefore, these QTLs were not disturbed by the A y allele. In contrast, Xzvdq1, Xzvdq3, and Xzvdq5 on chromosomes 1, X, and 12, respectively, showed high F2-type specificity (Tables1,2, and3 and Figure3A–C). In particular, Xzvdq3 was not identified, even as a suggestive QTL, in combined F2 mice. Therefore, it was expected that the Xzvdq3 would interact with the agouti genotype.
The interaction LOD score for Xzvdq3 was certainly high, but was below the significant threshold (Figure6). This suggests that the effect of Xzvdq3 is in same direction in both F2 non-A y and F2A y mice, but is substantially weaker in the presence of the A y allele. Intriguingly, the B6 allele at Xzvdq3 on X chromosome was associated with a higher grade of vacuolation not only in the parametric analysis for F2 non-A y mice, but also in the binary analysis. This indicates that the B6 allele exerts its effect more strongly than does the DDD allele for determining whether or not X-zone vacuolation develops.
Overall, four significant QTLs were identified for F2 non-A y mice and three significant QTLs were identified for F2A y mice. With regard to the number of QTLs responsible for X-zone vacuolation, Tanaka et al.[21] estimated that there would be three loci based on their study of SMXA recombinant inbred strains. The present results are in agreement with this estimation.
The suggestive effect of the agouti locus genotype on the X-zone vacuolation
A putative genetic link between the agouti locus genotype and X-zone vacuolation was suggested by Tanaka et al.[5, 6]. In the SM/J strain background at 70 days, mice with the A w /A w genotype had X-zones containing highly vacuolated cells, whereas mice with the A w /a or a/a genotypes had X-zones containing only non-vacuolated cells[5]. These results suggested the a allele suppressed X-zone vacuolation in a dominant manner.
In the BALB/c strain background at 70 days, mice with the A/A genotype had X-zones containing highly vacuolated cells, whereas BALB/c strain mice with the a/a genotype (coisogenic mutant occurring in the BALB/c strain) had X-zones containing only non-vacuolated cells[6]. However, at 140 days, BALB/c mice with both the A/A and a/a genotypes had X-zones containing only vacuolated cells. These results suggested that the a allele was not only involved with the extent of X-zone vacuolation, but also with the timing of X-zone degeneration. It should be noted that B6 females never develop X-zone vacuolation regardless of age, which is in contrast to a BALB/c background[17].
In this study, the F2 non-A y mice had A/a and a/a genotypes; the former was heterozygous and the latter was homozygous for the B6 allele. Similarly, the F2A y mice had A y /A and A y /a genotypes; the former was homozygous and the latter was heterozygous for the DDD allele. When the vacuolation grade was analyzed as a parametric or non-parametric trait, significant differences were observed between the two genotypes in both F2 types (Table6). The difference between genotypes was larger in F2 non-A y mice than in F2A y mice. In contrast to the Tanaka et al. proposal, the a allele suppressed vacuolation in a semidominant manner.
In this regard, it was also possible that the A allele promoted vacuolation. Thus, differences between the A y /A and A y /a alleles in F2A y mice was not as strong as that between the A/a and a/a alleles in F2 non-A y mice. In contrast, when the vacuolation grade was analyzed as a binary trait, the effect of the a allele was not significant in both F2 types. These results suggest that the agouti locus may have different genetic effects depending on different genetic background.
When the agouti locus genotypes were included as covariates by distinguishing four genotypes (A y /A, A y /a, A/a, and a/a), Xzvdq6 was identified on chromosome 8 as an interaction locus. When the agouti locus genotypes were included as covariates by distinguishing two genotypes (A y or non-A y ), Xzvdq6 was again identified. The patterns of LOD score plots were very similar for these two analyses. Therefore, whether or not mice had the A y allele was critically important for the interaction with Xzvdq6 on chromosome 8. This interaction may be one of the factors causing differences in the results in the aforementioned single QTL scans between F2 non-A y and F2A y mice.
These points of discussion are based on the assumption that Xzvdq2 is due to the agouti locus. However, the possibility that Xzvdq2 is due to another locus near to the agouti locus cannot be ruled out. Indeed, the agouti locus spans 259 kb on distal chromosome 2, and this region contains three other protein coding genes (Raly, Eif2s2, and Gm14226).
Candidate genes or loci
Shire and Spickett[4] reported the presence of an "earlier X-zone degeneration locus" (Ex), on chromosome 7. The Ex locus is involved with the timing of X-zone degeneration rather than the extent of X-zone vacuolation; therefore, Ex is irrelevant to this study because the B6 strain never develops X-zone vacuolation regardless of mouse age[3, 17, 22]. The genomic region surrounding Tyr locus on chromosome 7 in DDD-A y mice has been replaced by the B6 genome; DDD-A y mice had X-zones containing vacuolated cells in a manner similar to DDD mice.
Janat and Shire[17] reported the presence of two gene loci that controlled X-zone development on chromosomes 12 (Exz2) and 16 (Exz1) in CXB recombinant inbred strains, which were derived from C57BL/6By and BALB/cBy strains. Inferring from their linkage analysis, Exz2 may be located on distal chromosome 12; therefore, it may be allelic with the Xzvdq5.
Di Curzio and Goldowitz[7] addressed adrenal gland structure by QTL analysis. They analyzed X-zone widths in BXD recombinant inbred strains derived from the strains B6 and DBA/2J. They failed to identify any QTLs in females, but identified significant QTLs for X-zone width on chromosome 10 (Mxzwdq1) and 14 (Mxzwdq2) in males. Both of these QTLs did not coincide with those identified in this study.
Based on information retrieved from Mouse Genome Informatics, only a few genes or loci are currently recognized as having effects on the X-zone morphology. A possible candidate gene for Xzvdq1 on chromosome 1 is KiSS-1 metastasis-suppressor (Kiss1)[23]. Nuclear receptor subfamily 0, group B, member 1 (Nr0b1, also known as Dax1) was also a possible candidate gene for Xzvdq3 on the X chromosome[24]. In particular, Nr0b1 was cloned as the gene responsible for X-linked congenital adrenal hypoplasia in humans[25, 26]. Interestingly, loss of the function of Nr0b1 gene in humans and mice results in the lack of degeneration of the fetal zone and X-zone, respectively[24–26]. For these reasons, the fetal zone and X-zone are considered analogous structures[8].
Because Xzvdq6 interacts with an agouti genotype and is located on chromosome 8, the melanocortin 1 receptor (Mc1r) gene may be a possible candidate gene. Mc1r is located at the distal part of this chromosome (72.1 cM); therefore, Xzvdq6 is unlikely to be allelic with Mc1r because 95% CI for Xzvdq6 ranges from 21 to 34 cM. Adrenocortical dysplasia (Acd), which suppresses X-zone development, is also known to be located on chromosome 8. The Acd locus is at 53.04 cM on this chromosome and, therefore, is unlikely to be allelic with Xzvdq6.
X-zone morphology, including the extent of vacuolation, is determined genetically and, at the same time, modified hormonally[3]. Regarding hormonal factors, without doubt testosterone is critically important[27]. Testosterone administration induces X-zone degeneration, whereas gonadectomy preserves the X-zone in both sexes[28, 29]. However, the X-zone degenerates in testicular feminized male (Ar Tfm /Y) mice, suggesting that androgen is not necessarily required for X-zone degeneration. Nevertheless, androgen is still a critical factor for X-zone degeneration; therefore, genes that are controlled by androgen are possible candidate genes for the identified QTLs.
Despite efforts to elucidate critical roles of the X-zone, the physiological significance of this zone remains uncertain. Thus, it is most important to identify genes that account for the variations observed in X-zone morphology. Simultaneously, this study addressed the extent of X-zone vacuolation by adopting a genome-wide scan approach and showed that the extent of X-zone vacuolation in DDD females was controlled by multiple genes with complex interactions. The murine X-zone and the human fetal zone are considered analogous structures[8]. Therefore, the results of this study will aid in understanding function of not only of the X-zone but also of the human fetal zone. In addition, there is need to identify additional QTLs because there have been a limited number of genetic studies on adrenocortical morphology, including X-zone vacuolation. Identifying the genes responsible for the QTLs will be essential for understanding the molecular basis of X-zone function.
This work was funded in part by an institutional grant. Microsatellite genotyping, most of which had been performed for our previous studies using the same mice, was funded in part by a grant from Kieikai Research Foundation.
Authors' Affiliations
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Actors: Rocco Siffredi - Juelz Ventura
Categories: Hardcore - Big Breast - Anal - Huge dick - Tattoo/Piercing - HD Movies
Source: roccosiffredi
Description: Juelz Ventura's asshole gets stretched by Rocco's giant pole
Published on 2013-12-01
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Previous: Baby Hazel And Liam Valentines Day
Next: Naughty Pageant
Y8 Scooby Doo And The Creepy Castle Games Description: Scooby has three tries to find the gang. You lose a try if Scoobys fright factor gets too high but a Scooby snack can help which you will find inside the creepy castle.Open doors by clicking on them. You can also find objects inside that can help you


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قاتلة المجتمع
At Thirty-Five Three score and ten, the psalmist saith,And half my course is well-nigh run;I've had my flout at dusty death,I've had my whack of feast and fun.I've mocked at those who prate and preach;I've laughed with any man alive;But now with sobered heart I reachThe Great Divide of Thirty-five.And looking back I must confessI've little cause to feel elate.I've played the mummer more or less;I fumbled fortune, flouted fate.I've vastly dreamed and little done;I've idly watched my brothers strive:Oh, I have loitered in the sunBy primrose paths to Thirty-five!And those who matched me in the race,Well, some are out and trampled down;The others jog with sober pace;Yet one wins delicate renown.O midnight feast and famished dawn!O gay, hard life, with hope alive!O golden youth, forever gone,How sweet you seem at Thirty-five!Each of our lives is just a bookAs absolute as Holy Writ;We humbly read, and may not lookAhead, nor change one word of it.And here are joys and here are pains;And here we fail and here we thrive;O wondrous volume! what remainsWhen we reach chapter Thirty-five?The very best, I dare to hope,Ere Fate writes Finis to the tome;A wiser head, a wider scope,And for the gipsy heart, a home;A songful home, with loved ones near,With joy, with sunshine all alive:Watch me grow younger every year --Old Age! thy name is Thirty-five! Robert William Service
#!بسمم الله الرحمآن الرحيممً..# السلامم عليكمم ورحمتةة الله وبركآته .$وآآآآآو موضوعكك جميييلل جدا ~! يسلموو علي الموضوع الجميل..!@ننتذرمنك المزيد والمزيد من الابداععَ..!!وشكرآ لكي مره اخري علي هذا الموضوعع #!مع تحياتي لارواحكممم الطاهرةةة !جمولةة#!
شكرآ لكبإنتظآر كل جديد منكـ


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Tsw shifter for sale on US's largest classifieds sites (40 results)
1941 Willys Outlaw Body Art Morrison Pinched nose frame
E-Mail Questions at: firstname.lastname@example.com . One of a Kind 1941 Willy's Outlaw Coupe Multiple Award winning: Best Paint, Overall, Ect. This Car is absolutely a head turner, all hand build, outlaw fiberglass body, painted with House of Kolor Sunset Orange Pearl. Body seams are spot on all
2,165 Miles
1991 Nissan 300ZX in Tucson, AZ
1991 Nissan 300ZX in Tucson, AZ
Nissan 350Z Grand Touring Special Edition
For sale is a 2003 Nissan 350Z Grand Touring Special Edition competition car (near mint condition). Has a rare 2 tone custom paint job. Also, has won awards for \"best street look\" and \" best stock\" at two different 350z fests\'. Has a 3.5L V-6 engine with an AEM cold air intake system. A rear whe...
154,000 Miles
Hi I\'m selling my 08 WRX. this is a great car for the snow and it is definitely a lot of fun to drive. The boost peaks at 17 lbs. Reason I\'m getting rid of it is because I\'ve got my eyes on another car. this car has a few modifications... most of which was placed on by the previous owner. previous ...
112,000 Miles
Make: Subaru
Up for sale is my 2015 WRX Premium 6spd Manual. I bought the car new and am the original owner (26 years old, non smoker). The car has been great but recent changes have me considering getting something a bit larger. I\'ve babied the car and have performed my own maintenance in order to avoid any skim...
25,400 Miles
Year: 2015, Make: Subaru
Up for sale is my 2015 WRX Premium 6spd Manual. I bought the car new and am the original owner (26 years old, non smoker). The car has been great but recent changes have me considering getting something a bit larger. I\'ve babied the car and have performed my own maintenance in order to avoid any skim...
25,400 Miles
Year: 2015, Make: Subaru
1996 mustang gt 5speed with stage 2 clutch 4.6 119k mile v8 pi swap engine.k and n cold air with fender well hookup. Has a new pro 5.0 short throw shifter. Has new full length ceramic headers with h pipe and flow masters. New ford racing aluminum driveshaft. Has 19x10 rear wheels and 19x9 front afterm
2011 Ford Mustang GT Premium (PRICE REDUCED)
Up for sale is my 2011 Ford Mustang GT Premium. Really hate to sell the car but getting married soon and looking to buy a house so car needs to go. It has 61,000 miles on it. It has been garage kept and doesn\'t see rain or snow. It is fully loaded with back up camera, HID head lights, shaker 500 audi...
I have over 15k in upgrades. It\'s still easily a daily car I just didn\'t want to put miles on it. It has ported and polished heads, custom grind cam (sounds amazing) full exhaust, Vararam ram air intake, hotchkis stage 2 full suspension, 1000 hp axles, stage 3 twin disc clutch, under drive pulley, h...
29,900 Miles
Year: 2010
03 Honda Accord V6 6Speed with alot of aftermarket parts
I\'m selling my 03 Accord V6 6Speed, just turned 100k, 19in TSW Nagaros wrapped in Toyo Poxes. AEM V2 Cold Air Intake, Neuspeed Short Shifter, TEIN Adjustable coilovers, Ingalls Camber Correction kit (Rear), Richies J Pipe header, full GREDDY EVO2 catback exhaust with intermediate test pipe. McCull
100,000 Miles
WILL EDIT WITH INTERIOR PICS AFTER WORK 6000 OBOUp for sale is my 2001 Mustang GT. I\'ve owned it for 9 years now and loved every minute of it, but I\'ve just bought a new car and sadly have to get rid of the Mustang now. There are so many things done but I\'ll try to list the big item
133,000 Miles
Year: 2001
very rare nogaro blue s4 with the even rarer blue alcantara interior stage 3 mods recently installed at 130k and at same time timing belt and water pump done transmission rebuilt at 109k recent maintenance includes new cvc axels, wheel bearings, drive shaft, control arm and ball joints, big brake kit...
Year: 2000, Make: Aro
2000 BMW M5 with all the 2001 Model Upgrades! Full Screen Navigation!
****Please note that if you submit an offer and I accept it that the auction will be ended and you are committing to purchasing the vehicle. The only reason this vehicle is being relisted so quickly is because the buyer was not aware of this and simply just wanted to know if I would accept a certain p...
136,000 Miles
2012 Mustang GT 5.0 25k obo 18k miles
Up for sale is my 2012 Mustang GT 5.0 base with 18,300 miles, bought new from Mel Hambleton Ford. Asking 25k obo. 1 owner and never smoked in. Very quick and well maintained, lubrication engineers full synthetic used for last two oil changes done by the dealership. Mods: - SLP Loudmouth axleback - Cus...
18,300 Miles
92 Mazda MX-3 GS - CUSTOM!!! - $2000 (Salem, OR)
For sale is my 1992 Mazda MX-3 GS. I have a clean title to it and in hand. It has a whole lot of modifications and upgrades, totalling over $10,000, however I am willing to sell it for much less than that! Below is a list the details: Engine: JDM - KLZE (2.5L V6), with 200hp ** Factory engine ...
2006 Pontiac GTO HSV M6
There is no other car like this from top to bottom. Not only is this an OEM GM HSV urethane body imported from australia, the interior to the engine bay have been massaged and crafted for the perfect"Gotta have it car". The supsension, wheels, paint, body, interiro, exhaust have all been planned and...
52,000 Miles


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Geeks Petition for Free Lenovo BIOS
FOSS community acts to protect freedom of choice for laptop devices.
Software developer Evan Carroll and several coleagues have started a petition at Change.org calling for Lenovo to end its practice of building a hardware whitelist into the BIOS as a means of excluding devices the company does not support. Critics complain that this practice excludes some devices and drivers that the system actually supports, just because Lenovo doesn't happen to have a business agreement with the device vendor. In other words, the exclusion has no technical purpose. For instance, a wifi adapter by a manufacturer that is officially approved by Lenovo is allowed access to the system, but another adapter with an identical chipset is excluded by the BIOS.
The petition calls for Lenovo to release an "unencumbered" version of the BIOS and states "We request the option to purchase third-party miniPCI-E cards and install them into the laptops we purchased."
Issue 198/2017
Buy this issue as a PDF
Old Linux Kernel Bug Discovered
The bug was introduced back in 2009 and has been lurking around all this time.
OpenSSH 7.5 Released
The new release deprecates the sshd_config UsePrivilegeSeparation option.
Ubuntu Touch Not Dead
Lives on as a community project
Dell Launches Five New Linux Systems
Five new systems join Dell XPS 13 Developer Edition that come with Ubuntu pre-installed.
Microsoft Takes Skype for Linux Out of Alpha
The Skype Linux client now has almost the same capabilities that it enjoys on other platforms.
CeBIT 2017 OpenStack Day
At CeBIT 2017, OpenStack Day will offer a wide range of lectures and discussions.
Munich Plans to Ditch Linux and Go Back to Windows
A major setback for the Linux desktop.
Linux Kernel 4.10 Comes with New Security Features
Improved support for GPU in virtualization.
OpenSUSE Site Hacked
News site for the openSUSE community falls victim to a Wordpress exploit.
LibreOffice Goes Online with 5.3 Release
The source code is available online.


text: How to hack your air conditioner thermostat to make a wall explodeJesus Diaz4/01/14 1:49pmFiled to: wowboomhackrated rr592EditPromoteShare to KinjaToggle Conversation toolsGo to permalink GIF Sponsored by the game Watch Dogs, the folks at RatedRR explain how you can hack into anything in real life—from cameras to AC units to smart power outlets to wireless LED lights—to use those home devices as remote bomb detonators. And of course, their demonstration includes actual bombs and explosions for your cheering pleasure.SPLOID is a new blog about awesome stuff. Join us on FacebookReply59 repliesLeave a reply


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Whenever I have an emotion that makes me uncomfortable, I immediately try to think of someone I know (or know of) who's experienced the same thing. Is that person okay? Or close enough? Good. Then I will be too.
I think that started when I was a kid and realized I was going to die. For some reason, the fact that James Thurber had died made me feel better. I think I figured if there was an afterlife, then maybe I'd get to meet him. (This was before I'd figured out he was a major misogynist) And if there wasn't, well, he'd gone through it then I could handle it too.
And when I was going to have a baby, I was terrified. Yup, nearly everyone has a moment when she realizes that this very HUGE THING is going to have to exit her body and it is going to hurt. A lot. Someone pointed out that all I had to do was figure out that many cowards have more than one baby. So obviously, if it was that terrible, people would only have one, right?
That worked.
But then when I'm uncomfortable about my writing, I don't have any examples to turn to. I'm not sure why that is. Because I keep trying pretend that it isn't important to me? Because I keep trying to redefine failure? Because I don't know anyone else I think of as reasonable who keeps trying long after the world tells them to give it up? Because I'm a masochist? Because I have no clue what else I can do?
So in this case, it's time to turn to the other method of dealing: avoidance. Yay for avoidance, because as our ancestors knew, it's fine to not talk about it.
I think the cure for this state of mind is also going out and actually doing a job. Since I am not finding work, I think maybe it's time to get back to the refugees. Yeppers. Not for their sake, poor refugees. Because when I get in this brain, it's all about me.
You do know a lot of the time my mind manages to leave the navel gazing behind, right? Because I sort of figure the blog is about promo but it's also about the me me me me thing. Turns out when you're of a certain age, you (and maybe I mean me) don't need as much of that me me me me thing, which is good because there's no one else to provide that intense reassurance and patting and rapt attention unless you (and I mean me) are willing to pay good money for it -- from either a prostitute or therapist.
A blog is good enough. Cheaper too.


text:
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Hathor's Night on the Bench
— Hathor discovers what is under those robes.What I Did Last Thursday Night
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Find or become a nanny.
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We are looking for a nanny to start ASAP to care for our 12 month old son, four and a half days a week, Monday to Friday, 7:30 AM to 5:00 PM. Monday is a half day with variable daytim... Read More
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There's something about this curly-haired blonde that is quite sexy! She has nice, full breasts and a tight little tummy and bod! Her hair is disheveled, but she just looks like fun! I have to say, as a model, Nicole was definitely visually appealing. However, the first two masturbation to orgasm scenes with Nicole left me only lukewarm. Having said that, the third scene with the magic wand, where Nicole goes multiorgasmic, was really, really good!
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Now we have some variable to work with to give you some better understanding on the time scales for your website and rankings. So if i say 1a, this means a good domain age. If i say 3b this means the keyword difficulty is medium.
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1a + 2a + 3a + 4a + 5a = quicker chance to be ranked.
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1c + 2c + 3c + 4c + 5c = slower chance of getting ranked.
From these variables you can give yourself a very rough understanding on the current journey of your sites journey on Google and the level of SEO services you require.
Lets get this straight, no company who provides SEO services can guarantee you getting ranked on Google! The only company in the world that can make that guarantee is Google, and unfortunately, they do not provide search engine optimisation! There is no point asking an SEO company "what guarantees do you provide" as the answer from any reputable company would say none!
Its harsh i know but thats how it is. Any companies that state they guarantee google rankings you need to run for the hills as fast as possible because they simply cant make this promise. The likely hood is that they just use this phrase to get sales and not get you ranked anywhere. This is where people (maybe yourself) gets burnt.
Installing an SEO plugin such as Yoast doesn't mean your website is optimised! All this plugin, and others similar to it, does is make some settings on your website easier to do. You are probably looking at around 10-15% of the whole SEO management process these plugins cover for you. What about the other 85-90% of SEO work that needs to be performed to achieve the top search engine spots?!
The same applies if you have added keywords to your meta title & description, added keywords to your content & images etc… This isn't SEO management! This is just setting your meta tags! This is usually a sales tactic performed by website designers… "Do you want me to fully optimise your website to rank high on Google?". No, this alone doesn't mean your website is fully optimised at all. It really isnt worth paying a designer to do this as we have found it usually has a negative effect on clients rankings, as keywords are spammed and stuffed wherever they will go!
Backlinks are still the life line for any successful SEO campaign. You only need a small amount of high quality backlinks to ranked high. These links are hard to find and take a lot of time, effort, research and experience to get. These kind of links are the only links you should be focused on for your inbound link campaign.
Backlinks can easily be given or bought. Anything thats easy with inbound marketing is spam. It's that simple! If it was that easy to get links then everyone will be ranked in the top 3 on the search engines! These types of links need to be avoided at all costs as you will just throw your money away any probably have your website kicked off Google altogether.
Not Interested In Local Rankings
This is another phrase we hear a lot "We only want traffic from the UK, we're not interested in local search"! How Google ranks websites today is all about local search! If you search Google now for "SEO Services" you will find a mixture of results from national companies and local companies which are near your location. This local keyword trigger applies to nearly every search single term so if you ignore your local SEO, you're ignoring a lot of great traffic.
This local search trigger is aimed to help local businesses get more traffic. If Google wants to help you get more visitors, why wouldn't you take advantage of it!?
The content on your web pages is the driving force for your SEO campaign. Google loves quality content on ALL landing pages. You need to spend a lot of time planning and writing your content for more reasons than one!
To start with users want to know as much information as possible about your business, product/service and exactly what they get for their money. If users see a lack of content throughout a site doesn't look very professional and will lose a lot of user trust in your business and brand.
Another reason about having a good amount of great content per web page is that Google will pick out even more keywords and key phrases and rank them high on these search results. This results in more 'free' traffic that you wasn't even expecting. Content is the main resource at your disposal to tell Google exactly what you do and what you want to be ranked for.
Poor Websites
Free websites such as Wix, Weebly or any other types of these site builders to not rank on Google. The coding is that poor on these site builders they have no hope in performing well on Google, and to be honest, we have stopped taking SEO management orders from clients that have these types of sites. I know they come with 'SEO addons' that you can purchase but these are a joke. Don't waste your time or money with these site builders. We have had loads of success ranking WordPress websites so if you're looking for an easy platform to build a website, then WordPress is the one for you.
Google Penalties
Have you been naughty and tried to take short cuts to manipulate search engine rankings? You can come back from Google penalties but it can be a very long process. Depending on all the variables (mentioned above) it maybe easier and quicker to rank with a new domain and start from scratch. Once you're marked by Google it's very hard to shake off!
Google Updates
Google is constantly changing with new updates and algorithm changes to make a better user experience. It is important your SEO services keep up with these changes. Some of these updates may not effect your site but others may. It's important you have a good SEO company behind you to monitor and apply these changes.
Ranking at Position 1
Are you ranked at position 1 for all your keywords? Great! Just to double check, clear your browser cache and history and just perform another search. Still position one? A whopping 72% of people think they are ranked on the first page of Google when they are only viewing cached versions. If you keep searching Google on your keywords time and time again without clearing your cache then you will start seeing your website ranked there all the time!
If you are still ranked high after clearing your cache then should you stop your SEO management because you have reached your goals? Not at all! There will be 1000's of companies that want that position and will boost their SEO services in order to get it! If you stop your work they will take your spot and quickly start falling down the rankings like a lead balloon!


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"I am looking forward to increasing our participation in the fuel and lubricant distribution industry serving commercial and industrial customers," says Pilot CEO. Pilot Flying J closed its acquisition of a controlling interest in Maxum Petroleum for cash, and its contribution of Western Petroleum LLC into Maxum. Maxum will continue to operate as an independent
Pilot Flying J To Acquire Western Petroleum
"The acquisition of Western Petroleum's operations and employing Western Petroleum's talented workforce is a significant complement to an important aspect of our growth strategy," says Pilot Flying J CEO. Pilot Flying J is acquiring Western Petroleum LLC. Western Petroleum is a market leader in selling and transporting approximately 200 million gallons of fuel and lubricants


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Studying for the Microsoft 70-291 exam: "Windows 2003 Network Infrastructure". Question 1 at page 8-37 states:
DHCP server must have IP address 220.127.116.11 and this server lease addresses to the local network segment through the scope of 188.8.131.52
Which subnet mask can you assign to the server and scope that will place the DHCP server and clients on the same logical subnet and reserve the fewest number of bits possible for the host ID?
The way I went about this question was to test decreasing a C-class IP subnetmask (i.e. 255.255.255.0) by decreasing the third octed one bit at a time, and then AND:ing the third octet with 47 (third octet in DHCP static IP address) and 48 (third octet in DHCP client/lease addresses). Now, by testing with subnet masks 254, 252 etc all the way down to 224 I found that 224 would be the one. It is the correct answer.
I suspect this trial and error method of mine isn't optimal? Could anyone, please, suggest a smarter method?
Correct approach to determine subnet mask
By bohlinsitsupport
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At Wednesday social stitching, Janet had made this delightful dress for little Audrey.
Sue has just finished her seventh gorgeous "Shepherd's Bush" stocking for her latest little granddaughter.In the evening, the show and tell continued. It was lovely to see Barbara back in chilly Canberra after her lovely time away in the warmth with this fabulous quilt for her grandson. Lucky boy!
Linda has finished her bag from the retreat for a gift for a dear friend.
Margot has done a lovely job on the needlecase too.
Audrey though stole the show with lots of lovely finishes. She hasn't been able to come to class for a while but has been very busy stitching. Here are 2 of the delightful robots from Anni Downs'Boys book.
These beautiful purses have turned out so well. They are Anni's patterns as well. I do think there may be a kit for one in my stash!
Audrey had also finished this gorgeous pin cushion in the lovely woven wool felt.
Lastly, she has completed this beautiful needlecase. Wow! Well done, Audrey.
Sally had this beautiful library bag ready for her little granddaughter. It is just lovely and a nice use for the Snug as a Bug stitchery from Urban stitches.
So motivated to get stitching after seeing all those wonderful finishes!


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The article you requested is
Obesity and Associated Complications in Patients With Severe Mental Illnesses: A Cross-Sectional Survey
Margaret T. Susce, RN, MLT; Noemi Villanueva, PharmD; Francisco J. Diaz, PhD; and Jose de Leon, MD
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Background: This naturalistic cross-sectional survey of patients with severe mental illnesses explores the association between important variables and obesity, extreme obesity, diabetes mellitus type 2, hypertension, and hyperlipidemia in the clinical environment.
Method: Weight and height were obtained from 560 patients with severe mental illnesses (including DSM-IV schizophrenia, schizoaffective disorder, bipolar disorder, and major depressive disorder) at central Kentucky inpatient and outpatient facilities to estimate their body mass index (BMI). Chart diagnoses of diabetes mellitus, hypertension, and hyperlipidemia were obtained.
Results: When comparing the patients with severe mental illnesses with Kentucky adults from the general population, the odds ratio (OR) of obesity (BMI >= 30 kg/m2) was 2.6 (95% confidence interval [CI] = 2.2 to 3.0), and the OR of diabetes mellitus was 2.9 (95% CI = 2.3 to 3.6). Female gender, African American race, early start of psychiatric medication, and long psychiatric medication duration were significantly associated with obesity. Current alcohol and nicotine use exhibited significant ORs of obesity lower than 1, particularly in males. Obesity was closely associated with hypertension, type 2 diabetes mellitus, and hyperlipidemia. These complications were closely associated with each other and may indicate a further progression of obesity after aging.
Conclusions: These results suggest a complex pattern of variables that may influence the development of obesity and its complications in patients with severe mental illnesses, but they need replication. The major factors associated with obesity appear to be a long-term illness or treatment duration and substance use. The former may be more important in females, while the latter may be more important in males. Clinical diagnoses (schizophrenic or mood disorders) or current treatment did not appear to be fundamental factors.