text:
Tags Matching:
Originally published 03/21/2013
At Penn Station, seizing a chance to right a wrong
...For decades public officials and countless city skeptics have insisted nothing can be done. Penn Station is a black hole of politics, they say. The owners of Madison Square Garden, which squats on top of the station preventing real improvement, would never budge, especially not with the millions of dollars in tax breaks they've been getting or the nearly $1 billion they have poured into the aging arena.But there are precedents for achieving the impossible.
- Congress Has a History of Legislating in Secrecy
- At James Madison's home, slaves' lives matter as much as the man who owned them
- Destroying Great Mosque of al-Nuri 'is Isis declaring defeat'
- At Watergate, Recalling a Burglary That Toppled a President
- Hidden Trove of Suspected Nazi Artifacts Found in Argentina
- Researcher: "Actually, Yes It Is a Discovery If You Find Something in an Archive That No One Knew Was There."
- The Trump team is obsessing over Thucydides, the ancient historian who wrote a seminal tract on war
- Historians defend scholar who studies Poland and Holocaust
- Max Boot Says "Donald Trump Is Proving Too Stupid to Be President"
- Historians called on to write books that impact the public


text:
At its June 26 meeting, Saugatuck City Council was presented with a study done by Alexander Weiss Consulting regarding concerns with the current police department.
Rotted support beams due to the wrong materials used during construction led to the deck collapse earlier this month at Ottawa County Park's Weaver House, officials said.
The residents from four apartments in Grand Haven were forced to evacuate their building after a gas line was accidentally hit and opened early Tuesday afternoon, police said.
JAMESTOWN TWP. — A 14-year-old Allendale boy was injured when he was struck by a car he had recently vacated Monday night.
CLEVELAND — Coast Guard crews rescued 19 people and escorted 14 back to shore in four separate weather-related cases on Lake Erie involving capsized vessels on Saturday.
HOLLAND TWP. — A 40-year-old West Olive woman was in stable condition at Holland Hospital following a crash early Sunday afternoon on Waverly at Chicago Drive.
GEORGETOWN TWP. — An 8-year-old Jenison boy died Sunday, a day after he nearly drowned in a swimming pool at an Ottawa County residence, according to Sgt. Cal Keuning of the Ottawa County Sheriff's Department.
HOLLAND TWP. — A Muskegon woman and her 4-year-old child were injured in a two-vehicle crash in a Holland-area intersection at about 2:40 p.m. Friday.
A 16-year-old Holland-area boy was shot in the chest with a pellet gun and was treated for injuries at an area hospital.
POLKTON TWP. — A 16-year-old Grand Rapids girl said she was distracted talking to her friends when the vehicle she was driving rolled over Wednesday night.
A car and dump truck collided on Robbins Road in Grand Haven shortly before 6 p.m. today.
POLKTON TWP. — A Muskegon woman was injured when her vehicle was struck by another car Tuesday evening on I-96 near Coopersville.
A Spring Lake teen was sentenced to a month in jail and five years probation in what Ottawa County Circuit Judge Jon Hulsing said was a substantial deviation down from sentencing guidelines.
A 21-year-old Hope College student charged with sexually assaulting a female student while she was unconscious will spend the next 120 days in jail.
Employees of a Grand Haven auto parts store had a wide-open view of the street after a customer plowed her minivan into the building Monday morning.

subset: commoncrawl


text:
Publisher: John Wiley and Sons (Total: 1583 journals)
1 2 3 4 5 6 7 8 | Last [Sort by number of followers] [Restore default list]
1 2 3 4 5 6 7 8 | Last [Sort by number of followers] [Restore default list]
Animal Genetics
[SJR: 0.957] [H-I: 67] [8 followers] Follow
Hybrid journal (It can contain Open Access articles)
ISSN (Print) 0268-9146 - ISSN (Online) 1365-2052
Published by John Wiley and Sons [1583 journals]
- Genome-wide association analysis identifies potential regulatory genes for
eumelanin pigmentation in chicken plumage
- Authors: L. Yang; X. Du, S. Wei, L. Gu, N. Li, Y. Gong, S. Li
Abstract: Plumage color in chicken is determined by the proportion of eumelanin and pheomelanin pigmentation. As the main ingredient in plumage melanin, eumelanin plays a key role in the dark black, brown and grey coloration. However, very few studies have been performed to identify the related genes and mutations on a genome-wide scale. Herein, a resource family consisting of one backcross population and two F2 cross populations between a black roster and Yukou Brown I parent stockbreed was constructed for identification of genes related to eumelanin pigmentation. Chickens with eumelanin in their plumage were classified as the case group, and the rest were considered the control group. A genome-wide association study of this phenotype and genotypes using Affymetrix 600K HD SNP arrays in this F2 family revealed 13 significantly associated SNPs and in 10 separate genes on chromosomes 1, 2, 3 and 5. Based on previous studies in model species, we inferred that genes, including NUAK family kinase 1 (NUAK1) and sonic hedgehog (SHH), may play roles in the development of neural crest cells or melanoblasts during the embryonic period, which may also affect the eumelanin pigmentation. Our results facilitate the understanding of the genetic basis of eumelanin pigmentation in chicken plumage.
PubDate: 2017-06-22T04:48:25.207416-05:
DOI: 10.1111/age.12573
- Authors: L. Yang; X. Du, S. Wei, L. Gu, N. Li, Y. Gong, S. Li
- A post-GWAS confirming GPAT3 gene associated with pig growth and a
significant SNP influencing its promoter activity
- Authors: Kejun Wang; Yufang Liu, Qiao Xu, Chengkun Liu, Jve Wang, Cui Ding, Meiying Fang
Abstract: Our previous genome-wide association study revealed that a SNP, ASGA0040372, located upstream of glycerol-3-phosphate acyltransferase 3 (GPAT3), is related to growth traits in Duroc pigs. In this study, we aimed to extensively investigate the genetic variation of GPAT3 and to further verify the contribution of ASGA0040372 to growth traits and its effect on promoter activity. Our data, obtained based on gene cloning and rapid amplification of cDNA ends experiments, showed that there are 14 exons and three alternative splicing variants. Five SNPs were detected, including ASGA0040372 and four novel SNPs. Furthermore, the five SNPs obtained were used to confirm their genetic effects on pig growth by an association study in a Duroc pig population. The results indicated that three SNPs (ASGA0040372, RS331766122 and RS346215414) are highly associated with average daily gain or days to 100 kg traits (P-value < 0.05). Subsequent analysis suggested that the promoter region of GPAT3 gene, from –950 to –500 bp, exhibited the highest activity. We also observed that ASGA0040372 (A/G) alleles have significantly different effects on promoter transcriptional activities, which leads to differential expression of GPAT3. Taken together, we conclude that the GPAT3 gene is associated with pig growth and that ASGA0040372 influences promoter activity of GPAT3 in pig.
PubDate: 2017-06-09T05:31:56.184828-05:
DOI: 10.1111/age.12567
- Authors: Kejun Wang; Yufang Liu, Qiao Xu, Chengkun Liu, Jve Wang, Cui Ding, Meiying Fang
- Relationships between the genes expressed in the mesenteric adipose tissue
of beef cattle and feed intake and gain
- Authors: A. K. Lindholm-Perry; H. C. Cunningham, L. A. Kuehn, J. L. Vallet, J. W. Keele, A. P. Foote, K. M. Cammack, H. C. Freetly
Abstract: Mesenteric fat, a depot within the visceral fat, accumulates in cattle during maturation and finishing and may be a potential source of production inefficiency. The aim of this study was to determine whether the genes expressed in the mesenteric fat of steers were associated with body weight gain and feed intake. Sixteen steers chosen by their rank of distance from the bivariate mean for gain and feed intake were used for this study. Mesenteric fat was obtained and evaluated for differences in gene expression. A total of 1831 genes were identified as differentially expressed among steers with variation in feed intake and gain. Many of these genes were involved with metabolic processes such as proteolysis, transcription and translation. In addition, the Gene Ontology annotations including transport and localization were both over-represented among the differentially expressed genes. Pathway analysis was also performed on the differentially expressed genes. The superoxide radical degradation pathway was identified as over-represented based on the differential expression of the genes GPX7, SOD2 and TYRP1, suggesting a potential role for oxidative stress or inflammatory pathways among low gain–high intake animals. GPX7 and SOD2 were in lower transcript abundance, and TYRP1 was higher in transcript abundance among the low gain–high feed intake animals. The retinoate biosynthesis pathway was also enriched due to the differential expression of the genes AKR1C3, ALDH8A1, RDH8, RDH13 and SDR9C7. These genes were all more highly expressed in the low gain–high intake animals. The glycerol degradation and granzyme A signaling pathways were both associated with gain. Three glycerol kinase genes and the GZMA gene were differentially expressed among high vs. low gain animals. Mesenteric fat is a metabolically active tissue, and in this study, genes involved in proteolysis, transcription, translation, transport immune function, glycerol degradation and oxidative stress were differentially expressed among beef steers with variation in body weight gain and feed intake.
PubDate: 2017-05-31T22:10:30.202073-05:
DOI: 10.1111/age.12565
- Authors: A. K. Lindholm-Perry; H. C. Cunningham, L. A. Kuehn, J. L. Vallet, J. W. Keele, A. P. Foote, K. M. Cammack, H. C. Freetly
- Mitochondrial DNA analysis of ancient sheep from Altai
- Authors: M. A. Dymova; A. V. Zadorozhny, O. V. Mishukova, E. A. Khrapov, A. S. Druzhkova, V. A. Trifonov, I. G. Kichigin, A. A. Tishkin, S. P. Grushin, M. L. Filipenko
Abstract: A comparative analysis of the genetic diversity of ancient and modern sheep can shed light on the origin of these animals and their distribution as well as help to evaluate the role of humans at each formation stage of different sheep breeds. Here we isolated ancient DNA and performed sequencing of the mitochondrial DNA D-loop from 17 sheep bone remains (~4000–1000 years old) found in the archaeological complexes in the south of Altai (Western Siberia). The length of the sequences obtained ranged between 318 and 586 bp. The haplotype diversity and nucleotide diversity were 0.801 ± 0.081 and 0.0096 ± 0.0014 respectively. The average number of nucleotide differences was ~3.1. Nucleotide sequence analysis revealed that 15 specimens were nested within previously described A,B,C,D and E lineages and that two specimens had a basal position relative to the rest of the analyzed samples. A relatively high diversity of sheep haplotypes, including the presence of two basal haplotypes, indicates that the Altai region may have been a transport route of human migration. Further ancient DNA analysis of other specimens and deeper genome sequencing of samples with novel haplotypes is needed to better understand the demographic history of sheep in Southern Siberia.
PubDate: 2017-05-31T22:05:29.956508-05:
DOI: 10.1111/age.12569
- Authors: M. A. Dymova; A. V. Zadorozhny, O. V. Mishukova, E. A. Khrapov, A. S. Druzhkova, V. A. Trifonov, I. G. Kichigin, A. A. Tishkin, S. P. Grushin, M. L. Filipenko
- Genetic diversity of Syrian Arabian horses
- Authors: S. Almarzook; M. Reissmann, D. Arends, G. A. Brockmann
Abstract: Although Arabian horses have been bred in strains for centuries and pedigrees have been recorded in studbooks, to date, little is known about the genetic diversity within and between these strains. In this study, we tested if the three main strains of Syrian Arabian horses descend from three founders as suggested by the studbook. We examined 48 horses representing Saglawi (n = 18), Kahlawi (n = 16) and Hamdani (n = 14) strains using the Equine SNP70K BeadChip. For comparison, an additional 24 Arabian horses from the USA and three Przewalski's horses as an out group were added. Observed heterozygosis (Ho) ranged between 0.30 and 0.32, expected heterozygosity (He) between 0.30 and 0.31 and inbreeding coefficients (Fis) between −0.02 and −0.05, indicating high genetic diversity within Syrian strains. Likewise, the genetic differentiation between the three Syrian strains was very low (Fst
PubDate: 2017-05-31T22:05:28.490446-05:
DOI: 10.1111/age.12568
- Authors: S. Almarzook; M. Reissmann, D. Arends, G. A. Brockmann
- BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection
levels in Holstein and Holstein × Jersey crossbreed dairy cattle
- Authors: H. A. Carignano; M. J. Beribe, M. E. Caffaro, A. Amadio, J. P. Nani, G. Gutierrez, I. Alvarez, K. Trono, M. M. Miretti, M. A. Poli
Abstract: Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. This phenotypic variation appears to be determined by host genetics variants, especially those located in the BoLA-DRB3 MHCII molecule. We performed an association study using sequencing-based typed BOLA-DRB3 alleles from over 800 Holstein and Holstein × Jersey cows considering LI in vivo and accounting for filial relationships. The DBR3*0902 allele was associated with a low level of infection (LLI) (
PubDate: 2017-05-31T21:50:26.490817-05:
DOI: 10.1111/age.12566
- Authors: H. A. Carignano; M. J. Beribe, M. E. Caffaro, A. Amadio, J. P. Nani, G. Gutierrez, I. Alvarez, K. Trono, M. M. Miretti, M. A. Poli
- Characterization of a deletion in the Hsp70 cluster in the bovine
reference genome
- Authors: M. F. Suqueli García; M. A. Castellote, S. E. Feingold, P. M. Corva
Abstract: The 70 kilodalton heat shock proteins (Hsp70) are highly conserved molecular chaperones which have a crucial role in the stress response of the cell. In mammals, the Hsp70 proteins are encoded by a cluster of three genes: HSPA1A, HSPA1B and HSPA1L. In bovines, this cluster is located on chromosome 23 downstream of the major histocompatibility complex (BoLA). We detected inconsistencies in the location of markers on the Hsp70 genes reported in the literature that pointed to a potential deletion in the bovine reference genome UMD 3.1.1. An in silico analysis of the bovine genomic region of the Hsp70 cluster, using available information from public databases, confirmed the existence of a deletion of 11.1-kb spanning the HSPA1B gene and the intergenic region between HSPA1B and HSPA1A. Although we originally considered this an assembly error, it is most likely a particular condition of L1 Dominette 01449, the cow sequenced in the Bovine Genome Project. Moreover, we suggest a new classification of bovine Hsp70 sequences reported in NCBI and a reassignment of the location of SNPs from dbSNP that map to the deletion on BTA23. We also compared the location of selected transcription factor binding sites on the promoters of HSPA1A and HSPA1B. The results generated in the present work could be helpful to refine the reference genome of an important livestock species and also to understand the role and the regulation of the bovine Hsp70 genes.
PubDate: 2017-05-31T21:45:30.620579-05:
DOI: 10.1111/age.12561
- Authors: M. F. Suqueli García; M. A. Castellote, S. E. Feingold, P. M. Corva
- Estimations of linkage disequilibrium, effective population size and
ROH-based inbreeding coefficients in Spanish Churra sheep using imputed
high-density SNP genotypes
- Authors: P. K. Chitneedi; J. J. Arranz, A. Suarez-Vega, E. García-Gámez, B. Gutiérrez-Gil
Abstract: In this study, the availability of the Ovine HD SNP BeadChip (HD-chip) and the development of an imputation strategy provided an opportunity to further investigate the extent of linkage disequilibrium (LD) at short distances in the genome of the Spanish Churra dairy sheep breed. A population of 1686 animals, including 16 rams and their half-sib daughters, previously genotyped for the 50K-chip, was imputed to the HD-chip density based on a reference population of 335 individuals. After assessing the imputation accuracy for beagle v4.0 (0.922) and fimpute v2.2 (0.921) using a cross-validation approach, the imputed HD-chip genotypes obtained with beagle were used to update the estimates of LD and effective population size for the studied population. The imputed genotypes were also used to assess the degree of homozygosity by calculating runs of homozygosity and to obtain genomic-based inbreeding coefficients. The updated LD estimations provided evidence that the extent of LD in Churra sheep is even shorter than that reported based on the 50K-chip and is one of the shortest extents compared with other sheep breeds. Through different comparisons we have also assessed the impact of imputation on LD and effective population size estimates. The inbreeding coefficient, considering the total length of the run of homozygosity, showed an average estimate (0.0404) lower than the critical level. Overall, the improved accuracy of the updated LD estimates suggests that the HD-chip, combined with an imputation strategy, offers a powerful tool that will increase the opportunities to identify genuine marker-phenotype associations and to successfully implement genomic selection in Churra sheep.
PubDate: 2017-05-22T08:00:46.837411-05:
DOI: 10.1111/age.12564
- Authors: P. K. Chitneedi; J. J. Arranz, A. Suarez-Vega, E. García-Gámez, B. Gutiérrez-Gil
- Genetic variation and genetic structure of five Chinese indigenous pig
populations in Jiangsu Province revealed by sequencing data
- Authors: Q. Xiao; Z. Zhang, H. Sun, H. Yang, M. Xue, X. Liu, W. Zhang, Y. Zhen, M. Zhu, Q. Wang, Y. Pan
Abstract: In this study, we investigated the genetic variants, including SNPs and indels (short insertions or deletions, less than 50 bp in length), in the genomes and genetic structures of five pig populations (in the northern Taihu Lake region, Jiangsu Province) using the genotyping by genome reducing and sequencing (GGRS) approach. A total of 581 million good reads with an average depth of 11× and an average coverage of 2.16% were used to call variants. In general, 202 106 SNPs and 34 415 indels were obtained, of which 2690 SNPs and 224 indels were capable of inducing protein-coding changes. The genes containing these variants were extracted for functional annotation. The results of gene enrichment analysis revealed that the SNPs under investigation may be associated with reproduction, disease resistance, meat quality and adipose tissue traits, whereas the indels were associated mainly with adipose tissue and disease. Analysis of the genetic structure showed that each population displayed comparable, large differentiations from the others, indicating their uniqueness. In conclusion, the results of our study provide the first genomic overview of the genetic variants and population structures of five Chinese indigenous pig populations.
PubDate: 2017-05-22T07:50:27.516202-05:
DOI: 10.1111/age.12560
- Authors: Q. Xiao; Z. Zhang, H. Sun, H. Yang, M. Xue, X. Liu, W. Zhang, Y. Zhen, M. Zhu, Q. Wang, Y. Pan
- First study of mtDNA (D-loop) in Brazilian Caipira chickens
- Authors: M. E. Herkenhoff; F. M. Salzano, C. A. V. Lima-Rosa
PubDate: 2017-05-16T02:40:36.743517-05:
DOI: 10.1111/age.12562
- Authors: M. E. Herkenhoff; F. M. Salzano, C. A. V. Lima-Rosa
- Detecting selection signatures between Duroc and Duroc synthetic pig
populations using high-density SNP chip
- Authors: Z. Edea; J.-K. Hong, J.-H. Jung, D.-W. Kim, Y.-M. Kim, E.-S. Kim, S. S. Shin, Y. C. Jung, K.-S. Kim
Abstract: The development of high throughput genotyping techniques has facilitated the identification of selection signatures of pigs. The detection of genomic selection signals in a population subjected to differential selection pressures may provide insights into the genes associated with economically and biologically important traits. To identify genomic regions under selection, we genotyped 488 Duroc (D) pigs and 155 D × Korean native pigs (DKNPs) using the Porcine SNP70K BeadChip. By applying the FST and extended haplotype homozygosity (EHH-Rsb) methods, we detected genes under directional selection associated with growth/stature (DOCK7, PLCB4, HS2ST1, FBP2 and TG), carcass and meat quality (TG, COL14A1, FBXO5, NR3C1, SNX7, ARHGAP26 and DPYD), number of teats (LOC100153159 and LRRC1), pigmentation (MME) and ear morphology (SOX5), which are all mostly near or at fixation. These results could be a basis for investigating the underlying mutations associated with observed phenotypic variation. Validation using genome-wide association analysis would also facilitate the inclusion of some of these markers in genetic evaluation programs.
PubDate: 2017-05-16T02:35:30.077181-05:
DOI: 10.1111/age.12559
- Authors: Z. Edea; J.-K. Hong, J.-H. Jung, D.-W. Kim, Y.-M. Kim, E.-S. Kim, S. S. Shin, Y. C. Jung, K.-S. Kim
- Frame-shift variant in the CHRNE gene in a juvenile dog with suspected
myasthenia gravis-like disease
- Authors: Vanessa Herder; Malgorzata Ciurkiewicz, Wolfgang Baumgärtner, Vidhya Jagannathan, Tosso Leeb
PubDate: 2017-05-16T02:30:32.52574-05:0
DOI: 10.1111/age.12558
- Authors: Vanessa Herder; Malgorzata Ciurkiewicz, Wolfgang Baumgärtner, Vidhya Jagannathan, Tosso Leeb
- Isolation of subtelomeric sequences of porcine chromosomes for
translocation screening reveals errors in the pig genome assembly
- Authors: R. E. O'Connor; G. Fonseka, R. Frodsham, A. L. Archibald, M. Lawrie, G. A. Walling, D. K. Griffin
Abstract: Balanced chromosomal aberrations have been shown to affect fertility in most species studied, often leading to hypoprolificacy (reduced litter size) in domestic animals such as pigs. With an increasing emphasis in modern food production on the use of a small population of high quality males for artificial insemination, the potential economic and environmental costs of hypoprolific boars, bulls, rams etc. are considerable. There is therefore a need for novel tools to facilitate rapid, cost-effective chromosome translocation screening. This has previously been achieved by standard karyotype analysis; however, this approach relies on a significant level of expertise and is limited in its ability to identify subtle, cryptic translocations. To address this problem, we developed a novel device and protocol for translocation screening using subtelomeric probes and fluorescence in situ hybridisation. Probes were designed using BACs (bacterial artificial chromosomes) from the subtelomeric region of the short (p-arm) and long (q-arm) of each porcine chromosome. They were directly labelled with FITC or Texas Red (p-arm and q-arm respectively) prior to application of a 'Multiprobe' device, thereby enabling simultaneous detection of each individual porcine chromosome on a single slide. Initial experiments designed to isolate BACs in subtelomeric regions led to the discovery of a series of incorrectly mapped regions in the porcine genome assembly (from a total of 82 BACs, only 45 BACs mapped correctly). Our work therefore highlights the importance of accurate physical mapping of newly sequenced genomes. The system herein described allows for robust and comprehensive analysis of the porcine karyotype, an adjunct to classical cytogenetics that provides a valuable tool to expedite efficient, cost effective food production.
PubDate: 2017-05-12T05:06:47.457164-05:
DOI: 10.1111/age.12548
- Authors: R. E. O'Connor; G. Fonseka, R. Frodsham, A. L. Archibald, M. Lawrie, G. A. Walling, D. K. Griffin
- A novel mutation in the TYRP1 gene associated with brown coat colour in
the Australian Shepherd Dog Breed
- Authors: Evelina Hrckova Turnova; Zuzana Majchrakova, Marcela Bielikova, Katarina Soltys, Jan Turna, Andrej Dudas
PubDate: 2017-05-12T05:06:22.921621-05:
DOI: 10.1111/age.12563
- Authors: Evelina Hrckova Turnova; Zuzana Majchrakova, Marcela Bielikova, Katarina Soltys, Jan Turna, Andrej Dudas
- A survey of single nucleotide polymorphisms identified from whole-genome
sequencing and their functional effect in the porcine genome,
- Authors: B. N. Keel; D. J. Nonneman, G. A. Rohrer
Abstract: Genetic variants detected from sequence have been used to successfully identify causal variants and map complex traits in several organisms. High and moderate impact variants, those expected to alter or disrupt the protein coded by a gene and those that regulate protein production, likely have a more significant effect on phenotypic variation than do other types of genetic variants. Hence, a comprehensive list of these functional variants would be of considerable interest in swine genomic studies, particularly those targeting fertility and production traits. Whole-genome sequence was obtained from 72 of the founders of an intensely phenotyped experimental swine herd at the U.S. Meat Animal Research Center (USMARC). These animals included all 24 of the founding boars (12 Duroc and 12 Landrace) and 48 Yorkshire–Landrace composite sows. Sequence reads were mapped to the Sscrofa10.2 genome build, resulting in a mean of 6.1 fold (×) coverage per genome. A total of 22 342 915 high confidence SNPs were identified from the sequenced genomes. These included 21 million previously reported SNPs and 79% of the 62 163 SNPs on the PorcineSNP60 BeadChip assay. Variation was detected in the coding sequence or untranslated regions (UTRs) of 87.8% of the genes in the porcine genome: loss-of-function variants were predicted in 504 genes, 10 202 genes contained nonsynonymous variants, 10 773 had variation in UTRs and 13 010 genes contained synonymous variants. Approximately 139 000 SNPs were classified as loss-of-function, nonsynonymous or regulatory, which suggests that over 99% of the variation detected in our pigs could potentially be ignored, allowing us to focus on a much smaller number of functional SNPs during future analyses.
PubDate: 2017-05-08T22:25:26.593719-05:
DOI: 10.1111/age.12557
- Authors: B. N. Keel; D. J. Nonneman, G. A. Rohrer
- An evaluation of the International Society for Animal Genetics recommended
parentage and identification panel for the domestic pigeon (Columba livia
- Authors: M. Groot; W. A. Haeringen
Abstract: In this study, the International Society for Animal Genetics (ISAG) recommended panel for the identification of the domestic pigeon (Columba livia domestica) is characterized based on commonly used statistical parameters. The marker panel is based on 16 short tandem repeat (STR) loci (PIGN15, PIGN10, PIGN57, PIGN26, CliμD16, CliμD19, PIGN12, CliμD17, CliμT17, PIGN04, CliμD01, CliμD11, CliμD35, CliμT02, CliμT13, CliμT43). The alleles of the 16 loci consist of a mixture of tri-, tetra-, penta- and hexameric repeat patterns. A sex determination marker was included in the multiplex for quality control. The repeat sequence of the PIGN markers was previously unpublished and therefore sequenced to reveal the sequence pattern. In total, 1421 pigeons were genotyped on 16 STR loci to generate allele frequency data for each locus. For all 16 markers combined, a PE1 (combined non-exclusion probability, first parent) of 0.9986 and PE2 (combined non-exclusion probability, second parent) of >0.9999 was observed. Comparing the alleged father and mother, a PE value of >0.9999 was observed. Two of the markers, CliμD19 and PIGN12, were found to have relatively high Hardy–Weinberg equilibrium and F(null) values. Therefore these markers may be considered to be replaced by other STRs. Another point of discussion may be to add a gender identification marker to the recommended ISAG panel. Not only can this serve as an extra identification marker, but this can also confirm the sex of a sample, because it is challenging to determine the sex based on phenotypical characteristics, especially for chicks. In conclusion, the set of 16 STR markers can be used in routine parentage verification and the identification of individuals.
PubDate: 2017-04-27T13:26:23.027856-05:
DOI: 10.1111/age.12555
- Authors: M. Groot; W. A. Haeringen
- Whole genome sequencing reveals a novel deletion variant in the KIT gene
in horses with white spotted coat colour phenotypes
- Authors: N. Dürig; R. Jude, H. Holl, S. A. Brooks, C. Lafayette, V. Jagannathan, T. Leeb
Abstract: White spotting phenotypes in horses can range in severity from the common white markings up to completely white horses. EDNRB, KIT, MITF, PAX3 and TRPM1 represent known candidate genes for such phenotypes in horses. For the present study, we re-investigated a large horse family segregating a variable white spotting phenotype, for which conventional Sanger sequencing of the candidate genes' individual exons had failed to reveal the causative variant. We obtained whole genome sequence data from an affected horse and specifically searched for structural variants in the known candidate genes. This analysis revealed a heterozygous ~1.9-kb deletion spanning exons 10–13 of the KIT gene (chr3:77,740,239_77,742,136del1898insTATAT). In continuity with previously named equine KIT variants we propose to designate the newly identified deletion variant W22. We had access to 21 horses carrying the W22 allele. Four of them were compound heterozygous W20/W22 and had a completely white phenotype. Our data suggest that W22 represents a true null allele of the KIT gene, whereas the previously identified W20 leads to a partial loss of function. These findings will enable more precise genetic testing for depigmentation phenotypes in horses.
PubDate: 2017-04-26T06:35:36.306242-05:
DOI: 10.1111/age.12556
- Authors: N. Dürig; R. Jude, H. Holl, S. A. Brooks, C. Lafayette, V. Jagannathan, T. Leeb
- Haplotype structure and copy number polymorphism of the beta-defensin 7
genes in diverse chicken breeds
- Authors: M. O. Lee; M. N. Romanov, K. V. Plemyashov, N. V. Dementieva, O. V. Mitrofanova, O. Y. Barkova, J. E. Womack
Abstract: Beta-defensins is a family of avian peptides related to the innate immune system. Copy number variation was recently reported for the avian beta-defensin 7 gene (AvBD7) between the highly inbred Leghorn and Fayoumi lines. Here, we examined copy number variants in 35 different chicken breeds and found that 31 of them have at least the same representation of the duplicated AvBD7 allele. We also found haplotypes upstream of the AvBD6 regions that are strongly linked to the AvBD7 duplication. We observed a strong linkage disequilibrium spanning of the upstream region of the AvBD6 gene, with two SNPs being flanking markers to detect duplication of the AvBD7.
PubDate: 2017-04-05T05:20:32.700332-05:
DOI: 10.1111/age.12552
- Authors: M. O. Lee; M. N. Romanov, K. V. Plemyashov, N. V. Dementieva, O. V. Mitrofanova, O. Y. Barkova, J. E. Womack
- A novel splice mutation within equine KIT and the W15 allele in the
homozygous state lead to all white coat color phenotypes
- Authors: Heather M. Holl; Samantha A. Brooks, Meredith L. Carpenter, Carlos D. Bustamante, Christa Lafayette
PubDate: 2017-04-05T05:05:42.95485-05:0
DOI: 10.1111/age.12554
- Authors: Heather M. Holl; Samantha A. Brooks, Meredith L. Carpenter, Carlos D. Bustamante, Christa Lafayette
- Exclusion of known progressive retinal atrophy genes for blindness in the
Hungarian Puli
- Authors: Tracy Chew; Bianca Haase, Cali E. Willet, Claire M. Wade
PubDate: 2017-04-05T05:05:39.090111-05:
DOI: 10.1111/age.12553
- Authors: Tracy Chew; Bianca Haase, Cali E. Willet, Claire M. Wade
- Genotyping by sequencing reveals a new locus for pig teat number
- Authors: L. Wang; Y. Zhang, T. Zhang, L. Zhang, H. Yan, X. Liu, L. Wang
Abstract: Teat number is an extremely valuable trait for sow reproduction performance and piglet survival. Here, we used genotyping-by-sequencing and a general liner model to carry out genome-wide analysis of sow total teat number in an Erhualian population. The results indicated that eight SNPs on chromosomes 4, 5, 9 and 10 were significant genome wide (Bonferroni method, P
PubDate: 2017-04-03T01:25:46.316234-05:
DOI: 10.1111/age.12547
- Authors: L. Wang; Y. Zhang, T. Zhang, L. Zhang, H. Yan, X. Liu, L. Wang
- A novel 14-bp duplicated deletion within goat GHR gene is significantly
associated with growth traits and litter size
- Authors: Qing Yang; Hailong Yan, Jie Li, Han Xu, Ke Wang, Haijing Zhu, Hong Chen, Lei Qu, Xianyong Lan
PubDate: 2017-03-10T10:45:39.458027-05:
DOI: 10.1111/age.12551
- Authors: Qing Yang; Hailong Yan, Jie Li, Han Xu, Ke Wang, Haijing Zhu, Hong Chen, Lei Qu, Xianyong Lan
- Identification of the interaction between bta-miR-370 and OLR1 gene in
bovine adipocyte
- Authors: H. F. Li; S. H. Wang, Y. Guo, H. B. Zhao, X. Y. Li, X. Wang
Abstract: It has been shown that the oxidized low density lipoprotein receptor 1 (OLR1) gene plays an important role in the degradation of oxidized low density lipoprotein. Previous studies found a SNP in the 3′-untranslated region (3′-UTR) of the OLR1 gene associated with milk production traits in different dairy cattle populations and with loin eye area and marbling depth in beef cattle. MicroRNAs can regulate gene expression by binding the 3′-UTR of target genes to degrade or to repress the translation of target genes. Bioinformatics have shown that there is a binding site of bta-miR-370 in the 3′-UTR of the OLR1 gene, and a previous luciferase reporter assay system showed that the A/C mutation occurring in the 3′-UTR of this gene caused the binding sites of bta-miR-370 to disappear in HEK293 cells. To further validate whether OLR1 was the target gene of bta-miR-370, the over-expression and interference expression of bta-miR-370 were determined by transfecting bta-miR-370 mimics and inhibitor supplementations into bovine adipocyte. The qRT-PCR result showed that the relative expression of OLR1 gene significantly decreased in the mimics group compared to the control, whereas the expression level in inhibitor group was higher than its control group. The above results were further verified by a Western blot at the protein level. In addition, lipid formation analysis of bovine adipocytes was performed via oil red O staining, and we found that cytoplasm lipid droplets in the inhibitor group showed a tendency to increase compared to the control group, whereas in the mimics group, we observed an obvious decrease of cytoplasm lipid droplets compared to the control and inhibitor groups. Taken together, our data here suggest that bta-miR-370 has a negative regulation role for OLR1 both at the gene expression and protein levels and bovine adipocytes cytoplasm lipid droplets formation, which provides a reference for illustrating how the OLR1 gene affects milk production and beef quality traits in cattle.
PubDate: 2017-03-10T10:45:15.700944-05:
DOI: 10.1111/age.12550
- Authors: H. F. Li; S. H. Wang, Y. Guo, H. B. Zhao, X. Y. Li, X. Wang
- An atlas and analysis of bovine skeletal muscle long noncoding RNAs
- Authors: X. F. Liu; X. B. Ding, X. Li, C. F. Jin, Y. W. Yue, G. P. Li, H. Guo
Abstract: Long noncoding RNAs (lncRNAs) have various biological functions and have been extensively studied in recent years. However, the identification and characterization of bovine lncRNAs in skeletal muscle has been very limited compared with that of lncRNAs in other model organisms. In this study, 7188 bovine skeletal muscle lncRNAs were identified by RNA-Seq and a stringent screening procedure in four different muscle tissues. These lncRNAs shared many characteristics with other mammalian lncRNAs, such as a shorter open reading frame and lower expression level than for mRNAs. Furthermore, the chromosomal locations and global expression patterns for these lncRNAs are also described in detail. More importantly, we detected the important interaction relationships of lncRNAs–miRNAs–mRNAs related to muscle development among 36 lncRNAs, 62 miRNAs and 12 mRNAs. Our results provide a global expression pattern of lncRNAs specific to bovine skeletal muscle and provide important targets for revealing the function of bovine muscle development by thoroughly studying the interaction relationships of lncRNAs–miRNAs–mRNAs.
PubDate: 2017-03-06T06:05:55.123278-05:
DOI: 10.1111/age.12539
- Authors: X. F. Liu; X. B. Ding, X. Li, C. F. Jin, Y. W. Yue, G. P. Li, H. Guo
- Identification of a new Y chromosome haplogroup in Spanish native cattle
- Authors: R. Pelayo; M. C. T. Penedo, M. Valera, A. Molina, L. Millon, C. Ginja, L. J. Royo
Abstract: The aim of this work was to perform a thorough analysis of the diversity of Y-haplotypes in Spanish cattle. A total of 207 Bos taurus males were sampled across 25 European breeds, with a special focus on rare, local Spanish populations. Animals were genotyped with five Y-specific microsatellites (INRA189, UMN0103, UMN0307, BM861 and BYM1), two indels (ZFY10 and USP9Y) and one SNP (UTY19). A new haplogroup, distinct from those described by Götherström et al. (2005), was identified and named Y1.2. Samples representing the three B. taurus Y-haplogroups were genotyped for four additional Y chromosome SNPs (rs121919254, rs121919281, rs121919323 and rs137049553). Among these SNPs, only rs121919281 was informative in B. taurus and helped to confirm the new Y1.2 haplogroup. Analysis of a larger dataset of standardized haplotypes for 1507 individuals from 57 populations from Spain, other European countries and Africa showed the new Y1.2 haplogroup to be found exclusively in Spanish breeds. This finding reinforces the importance of local Spanish cattle as reservoirs of genetic diversity as well as the importance of the Iberian Peninsula in the history of cattle.
PubDate: 2017-02-27T23:55:25.897878-05:
DOI: 10.1111/age.12549
- Authors: R. Pelayo; M. C. T. Penedo, M. Valera, A. Molina, L. Millon, C. Ginja, L. J. Royo
- Sex chromosomal abnormalities associated with equine infertility:
validation of a simple molecular screening tool in the Purebred Spanish
- Authors: G. Anaya; A. Molina, M. Valera, M. Moreno-Millán, P. Azor, P. Peral-García, S. Demyda-Peyrás
Abstract: Chromosomal abnormalities in the sex chromosome pair (ECAX and ECAY) are widely associated with reproductive problems in horses. However, a large proportion of these abnormalities remains undiagnosed due to the lack of an affordable diagnostic tool that allows for avoiding karyotyping tests. Hereby, we developed an STR (single-tandem-repeat)-based molecular method to determine the presence of the main sex chromosomal abnormalities in horses in a fast, cheap and reliable way. The frequency of five ECAX-linked (LEX026, LEX003, TKY38, TKY270 and UCDEQ502) and two ECAY-linked (EcaYH12 and SRY) markers was characterized in 261 Purebred Spanish Horses to determine the efficiency of the methodology developed to be used as a chromosomal diagnostic tool. All the microsatellites analyzed were highly polymorphic, with a sizeable number of alleles (polymorphic information content > 0.5). Based on this variability, the methodology showed 100% sensitivity and 99.82% specificity to detect the most important sex chromosomal abnormalities reported in horses (chimerism, Turner's syndrome and sex reversal syndromes). The method was also validated with 100% efficiency in 10 individuals previously diagnosed as chromosomally aberrant. This STR screening panel is an efficient and reliable molecular–cytogenetic tool for the early detection of sex chromosomal abnormalities in equines that could be included in breeding programs to save money, effort and time of veterinary practitioners and breeders.
PubDate: 2017-02-22T01:40:44.620824-05:
DOI: 10.1111/age.12543
- Authors: G. Anaya; A. Molina, M. Valera, M. Moreno-Millán, P. Azor, P. Peral-García, S. Demyda-Peyrás
- Relationships between inflammation- and immunity-related transcript
abundance in the rumen and jejunum of beef steers with divergent average
daily gain
- Authors: J. G. Reynolds; A. P. Foote, H. C. Freetly, W. T. Oliver, A. K. Lindholm-Perry
Abstract: The bovine rumen papillae are in contact with a wide array of microorganisms and the metabolites they produce, which may activate an inflammatory and/or immune response. Cytokines, chemokines and their receptor genes were tested for differential expression in the rumen and jejunum of beef steers with greater and lesser average daily body weight gain (ADG) near the average daily dry matter intake (DMI) for the population. Angus-sired steers (n = 16) were used to represent the greater (ADG = 2.2 ± 0.07 kg/day; DMI = 10.1 ± 0.05 kg/day) and lesser (ADG = 1.7 ± 0.05 kg/day; DMI = 10.1 ± 0.05 kg/day) ADG groups with eight steers each. Rumen epithelium and jejunum mucosal samples were collected at slaughter, and gene expression was evaluated using a commercially available qRT-PCR array containing 84 genes representing chemokines, cytokines and their receptors. None of the genes on the array were differentially expressed in the jejunum of the steers with greater vs. lesser ADG. However, in the rumen, two chemokine genes (CCL11, CXCL5) and one receptor gene (IL10RA) were detected as differentially expressed (P
PubDate: 2017-02-21T00:30:22.573568-05:
DOI: 10.1111/age.12546
- Authors: J. G. Reynolds; A. P. Foote, H. C. Freetly, W. T. Oliver, A. K. Lindholm-Perry
- Genomic prediction of reproduction traits for Merino sheep
- Authors: S. Bolormaa; D. J. Brown, A. A. Swan, J. H. J. Werf, B. J. Hayes, H. D. Daetwyler
Abstract: Economically important reproduction traits in sheep, such as number of lambs weaned and litter size, are expressed only in females and later in life after most selection decisions are made, which makes them ideal candidates for genomic selection. Accurate genomic predictions would lead to greater genetic gain for these traits by enabling accurate selection of young rams with high genetic merit. The aim of this study was to design and evaluate the accuracy of a genomic prediction method for female reproduction in sheep using daughter trait deviations (DTD) for sires and ewe phenotypes (when individual ewes were genotyped) for three reproduction traits: number of lambs born (NLB), litter size (LSIZE) and number of lambs weaned. Genomic best linear unbiased prediction (GBLUP), BayesR and pedigree BLUP analyses of the three reproduction traits measured on 5340 sheep (4503 ewes and 837 sires) with real and imputed genotypes for 510 174 SNPs were performed. The prediction of breeding values using both sire and ewe trait records was validated in Merino sheep. Prediction accuracy was evaluated by across sire family and random cross-validations. Accuracies of genomic estimated breeding values (GEBVs) were assessed as the mean Pearson correlation adjusted by the accuracy of the input phenotypes. The addition of sire DTD into the prediction analysis resulted in higher accuracies compared with using only ewe records in genomic predictions or pedigree BLUP. Using GBLUP, the average accuracy based on the combined records (ewes and sire DTD) was 0.43 across traits, but the accuracies varied by trait and type of cross-validations. The accuracies of GEBVs from random cross-validations (range 0.17–0.61) were higher than were those from sire family cross-validations (range 0.00–0.51). The GEBV accuracies of 0.41–0.54 for NLB and LSIZE based on the combined records were amongst the highest in the study. Although BayesR was not significantly different from GBLUP in prediction accuracy, it identified several candidate genes which are known to be associated with NLB and LSIZE. The approach provides a way to make use of all data available in genomic prediction for traits that have limited recording.
PubDate: 2017-02-17T01:25:39.677657-05:
DOI: 10.1111/age.12541
- Authors: S. Bolormaa; D. J. Brown, A. A. Swan, J. H. J. Werf, B. J. Hayes, H. D. Daetwyler
- PCK1 expression is correlated with the plasma glucose level in the duck
- Authors: L. Chen; T. Zeng, G. Q. Li, R. Liu, Y. Tian, Q. H. Li, L. Z. Lu
Abstract: Phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1) is a key gene in gluconeogenesis and glyceroneogenesis. Although its functions have been extensively studied in mice, bats and humans, little is known in ducks. Here, PCK1 functions were studied using a duck domestication model and a 48-h fasting experiment. We found PCK1 expression significantly decreased in two breeds of domestic ducks (Jinyun Pockmark ducks and Cherry Valley ducks) as compared with wild ducks (Anas platyrhynchos). Simultaneously, plasma levels of glucose, triglycerides and free fatty acid in domestic ducks were lower than in wild ducks. When compared with fed ducks, the plasma triglyceride level was observed to be significantly decreased, while the glucose and free fatty acid levels remained constant in 48-h fasting ducks. The expression analysis of gluconeogenic genes revealed that fructose-1,6-bisphosphatase genes (FBP1 and FBP2) and the glucose-6-phosphatase gene (G6PC2) were not changed, whereas PCK1 was significantly upregulated. In addition, the reported regulators of PCK1, including forkhead box A2 (FOXA2) gene and orphan nuclear receptor NR4A family genes (NR4A1, NR4A2 and NR4A3), exhibited similar expression levels between 48-h fasting ducks and fed ducks, suggesting that PCK1 is not regulated by these genes in the duck under fasting conditions. In conclusion, PCK1 expression may affect plasma levels of glucose, triglycerides and free fatty acid during the duck domestication process. This work demonstrates for the first time in duck that PCK1 is a key gene in maintaining plasma glucose homeostasis during fasting and that the upregulated expression of PCK1 may be responsible for constant plasma free fatty acid level by the glyceroneogenesis process.
PubDate: 2017-02-15T02:10:26.012768-05:
DOI: 10.1111/age.12540
- Authors: L. Chen; T. Zeng, G. Q. Li, R. Liu, Y. Tian, Q. H. Li, L. Z. Lu
- ASIP gene variation in Chinese donkeys
- Authors: Ting Sun; Shiying Li, Xiaoting Xia, Chuanliang Ji, Shanyi Shen, Guoliang Zhang, Jie Yu, Guimiao Jiang, Ruihua Dang, Chuzhao Lei
PubDate: 2017-02-15T02:05:26.625037-05:
DOI: 10.1111/age.12530
- Authors: Ting Sun; Shiying Li, Xiaoting Xia, Chuanliang Ji, Shanyi Shen, Guoliang Zhang, Jie Yu, Guimiao Jiang, Ruihua Dang, Chuzhao Lei
- Identification of the crucial genes in the elimination and survival
process of Salmonella enterica ser. Pullorum in the chicken spleen
- Authors: T. Ma; L. Xu, H. Wang, X. Guo, Z. Li, F. Wan, J. Chen, L. Liu, X. Liu, G. Chang, G. Chen
Abstract: Salmonella enterica ser. Pullorum is one of the most easily re-infecting pathogens in poultry production because of its mechanism of escaping from immune elimination. We used the transcriptome method to investigate the variation in gene expression in chicken spleen resulting from the interaction between hosts and S. Pullorum in the survival process. The expression of various genes related to the maturation and activation of B cells was activated before S. Pullorum was eliminated, which might help S. Pullorum escape from the elimination process. The suppression of some genes involved in the fusion of autophagosomes and lysosomes, such as MYO6, was identified and may be regulated by the secretion systems of S. Pullorum. In addition, a large proportion of these differentially expressed genes could be localized in the identified quantitative trait loci regions associated with the antibody response to bacteria. Collectively, these identified genes provided an outline for further understanding the interaction between chicken immune cells and S. Pullorum in chicken spleen.
PubDate: 2017-02-07T23:51:28.809475-05:
DOI: 10.1111/age.12533
- Authors: T. Ma; L. Xu, H. Wang, X. Guo, Z. Li, F. Wan, J. Chen, L. Liu, X. Liu, G. Chang, G. Chen
- A genome-wide association study for a proxy of intermuscular fat level in
the Italian Large White breed identifies genomic regions affecting an
important quality parameter for dry-cured hams
- Authors: L. Fontanesi; G. Schiavo, G. Galimberti, S. Bovo, V. Russo, M. Gallo, L. Buttazzoni
Abstract: Intermuscular fat content in protected designations of origin dry-cured hams is a very important meat quality trait that affects the acceptability of the product by the consumers. An excess in intermuscular fat (defined as the level of fat deposition between leg muscles) is a defect that depreciates the final product. In this study we carried out a genome-wide association study for visible intermuscular fat (VIF) of hams in the Italian Large White pig breed. This trait was evaluated on the exposed muscles of green legs in 1122 performance-tested gilts by trained personnel, according to a classification scale useful for routine and cheap evaluation. All animals were genotyped with the Illumina PorcineSNP60 BeadChip. The genome-wide association study identified three QTL regions on porcine chromosome 1 (SSC1; accounting for ~79% of the SNPs below the 5.0E−04 threshold) and SSC2, two on SSC7 and one each on SSC3, SSC6, SSC9, SSC11, SSC13, SSC15, SSC16 and SSC17. The most significant SNP (ALGA0004143 on SSC1 at 77.3 Mb; PFDR
PubDate: 2017-02-03T06:25:44.977614-05:
DOI: 10.1111/age.12542
- Authors: L. Fontanesi; G. Schiavo, G. Galimberti, S. Bovo, V. Russo, M. Gallo, L. Buttazzoni
- A genome-wide association analysis for carcass traits in a commercial
Duroc pig population
- Authors: P. G. Eusebi; R. González-Prendes, R. Quintanilla, J. Tibau, T. F. Cardoso, A. Clop, M. Amills
Abstract: We performed a genome-wide association study to map the genetic determinants of carcass traits in 350 Duroc pigs typed with the Porcine SNP60 BeadChip. Association analyses were carried out using the gemma software. The proportion of phenotypic variance explained by the SNPs ranged between negligible to moderate (hSNP2= 0.01–0.30) depending on the trait under consideration. At the genome-wide level, we detected one significant association between backfat thickness between the 3rd and 4th ribs and six SNPs mapping to SSC12 (37–40 Mb). We also identified several chromosome-wide significant associations for ham weight (SSC11: 51–53 Mb, three SNPs; 67–68 Mb, two SNPs), carcass weight (SSC11: 66–68 Mb, two SNPs), backfat thickness between the 3rd and 4th ribs (SSC12: 21 Mb, one SNP; 33–40 Mb, 17 SNPs; 51–58 Mb, two SNPs), backfat thickness in the last rib (SSC12: 37 Mb, one SNP; 40–41 Mb, nine SNPs) and lean meat content (SSC13: 34 Mb, three SNPs and SSC16: 45.1 Mb, one SNP; 62–63 Mb, 10 SNPs; 71–75 Mb, nine SNPs). The ham weight trait-associated region on SSC11 contains two genes (UCHL3 and LMO7) related to muscle development. In addition, the ACACA gene, which encodes an enzyme for the catalysis of fatty acid synthesis, maps to the SSC12 (37–41 Mb) region harbouring trait-associated regions for backfat thickness traits. Sequencing of these candidate genes may help to uncover the causal mutations responsible for the associations found in the present study.
PubDate: 2017-02-03T06:25:33.039352-05:
DOI: 10.1111/age.12545
- Authors: P. G. Eusebi; R. González-Prendes, R. Quintanilla, J. Tibau, T. F. Cardoso, A. Clop, M. Amills
- A novel VWF variant associated with type 2 von Willebrand disease in
German Wirehaired Pointers and German Shorthaired Pointers
- Authors: M. Vos-Loohuis; B. A. Oost, C. Dangel, I. Langbein-Detsch, P. A. Leegwater
Abstract: Von Willebrand disease (VWD), caused by deficiency of the von Willebrand factor (VWF), is the most common bleeding disorder in humans and dogs. The complete cDNA encoding VWF of a German Wirehaired Pointer with type 2 VWD was sequenced, and we found four variants that alter the amino acid sequence. These variants were: c.1657T>G corresponding to p.Trp553Gly; c.1777G>A (p.Glu593Lys); c.4937A>G (p.Asn1646Ser) and c.5544G>A (p.Met1848Ile). A haplotype of the c.1657G, c.1777A and c.4937G alleles co-segregated with the VWF antigen level in a four-generation pedigree with the disease. Healthy dogs of the breed were found that were homozygous for the c.1777A or the c.5544A allele, indicating that these variants do not cause VWD. Dogs that were homozygous for the c.4937G allele and had no signs of a bleeding disorder were observed in the Chinese Crested dog breed. Thus, only the c.1657G variant was found in the homozygous state exclusively in VWD affecteds, and this variant is the strongest candidate to be the cause of VWD type 2 in the German Wirehaired Pointer breed. A screen of German Shorthaired Pointers indicated that the variant also segregates with VWD in this breed.
PubDate: 2017-02-03T06:25:31.472668-05:
DOI: 10.1111/age.12544
- Authors: M. Vos-Loohuis; B. A. Oost, C. Dangel, I. Langbein-Detsch, P. A. Leegwater
- Fine mapping of a distal chromosome 4 QTL affecting growth and muscle mass
in a chicken advanced intercross line
- Authors: S. Lyu; D. Arends, M. K. Nassar, G. A. Brockmann
Abstract: In our previous research, QTL analysis in an F2 cross between the inbred New Hampshire (NHI) and White Leghorn (WL77) lines revealed a growth QTL in the distal part of chromosome 4. To physically reduce the chromosomal interval and the number of potential candidate genes, we performed fine mapping using individuals of generations F10, F11 and F12 in an advanced intercross line that had been established from the initial F2 mapping population. Using nine single nucleotide polymorphism (SNP) markers within the QTL region for an association analysis with several growth traits from hatch to 20 weeks and body composition traits at 20 weeks, we could reduce the confidence interval from 26.9 to 3.4 Mb. Within the fine mapped region, markers rs14490774, rs314961352 and rs318175270 were in full linkage disequilibrium (D′ = 1.0) and showed the strongest effect on growth and muscle mass (LOD ≥ 4.00). This reduced region contains 30 genes, compared to 292 genes in the original region. Chicken 60 K and 600 K SNP chips combined with DNA sequencing of the parental lines were used to call mutations in the reduced region. In the narrowed-down region, 489 sequence variants were detected between NHI and WL77. The most deleterious variants are a missense variant in ADGRA3 (SIFT = 0.02) and a frameshift deletion in the functional unknown gene ENSGALG00000014401 in NHI chicken. In addition, five synonymous variants were discovered in genes PPARGC1A, ADGRA3, PACRGL, SLIT2 and FAM184B. In our study, the confidence interval and the number of potential genes could be reduced 8- and 10- fold respectively. Further research will focus on functional effects of mutant genes.
PubDate: 2017-01-26T04:56:30.715408-05:
DOI: 10.1111/age.12532
- Authors: S. Lyu; D. Arends, M. K. Nassar, G. A. Brockmann
- Novel class II alpha MHC variability in a small peripheral Atlantic salmon
- Authors: Kate L. Ciborowski; William C. Jordan, Carlos García de Leániz, Sofia Consuegra
PubDate: 2017-01-24T00:56:06.193729-05:
DOI: 10.1111/age.12535
- Authors: Kate L. Ciborowski; William C. Jordan, Carlos García de Leániz, Sofia Consuegra
- Limited representation of OMIA causative mutations for cattle in SNP
- Authors: Aditi Sharma; Yongmin Cho, Bong-Hwan Choi, Han-Ha Chai, Jong-Eun Park, Dajeong Lim
PubDate: 2017-01-24T00:00:39.591418-05:
DOI: 10.1111/age.12534
- Authors: Aditi Sharma; Yongmin Cho, Bong-Hwan Choi, Han-Ha Chai, Jong-Eun Park, Dajeong Lim
- Deletion of 2.7 kb near HOXD3 in an Arabian horse with
occipitoatlantoaxial malformation
- Authors: M. H. Bordbari; M. C. T. Penedo, M. Aleman, S. J. Valberg, J. Mickelson, C. J. Finno
Abstract: In the horse, the term occipitoatlantoaxial malformation (OAAM) is used to describe a developmental defect in which the first cervical vertebra (atlas) resembles the base of the skull (occiput) and the second cervical vertebra (axis) resembles the atlas. Affected individuals demonstrate an abnormal posture and varying degrees of ataxia. The homeobox (HOX) gene cluster is involved in the development of both the axial and appendicular skeleton. Hoxd3-null mice demonstrate a strikingly similar phenotype to Arabian foals with OAAM. Whole-genome sequencing was performed in an OAAM-affected horse (OAAM1) and seven unaffected Arabian horses. Visual inspection of the raw reads within the region of HOXD3 identified a 2.7-kb deletion located 4.4 kb downstream of the end of HOXD4 and 8.2 kb upstream of the start of HOXD3. A genotyping assay revealed that both parents of OAAM1 were heterozygous for the deletion. Additional genotyping identified two of 162 heterozygote Arabians, and the deletion was not present in 371 horses of other breeds. Comparative genomics studies have revealed that this region is highly conserved across species and that the entire genomic region between Hoxd4 and Hoxd3 is transcribed in mice. Two additional Arabian foals diagnosed with OAAM (OAAM 2 and 3) were genotyped and did not have the 2.7-kb deletion. Closer examination of the phenotype in these cases revealed notable variation. OAAM3 also had facial malformations and a patent ductus arteriosus, and the actual malformation at the craniocervical junction differed. Genetic heterogeneity may exist across the HOXD locus in Arabian foals with OAAM.
PubDate: 2017-01-23T02:00:27.135192-05:
DOI: 10.1111/age.12531
- Authors: M. H. Bordbari; M. C. T. Penedo, M. Aleman, S. J. Valberg, J. Mickelson, C. J. Finno
- Development of Diversity Arrays Technology markers as a tool for rapid
genomic assessment in Nile tilapia, Oreochromis niloticus
- Authors: C. E. Lind; A. Kilian, J. A. H. Benzie
Abstract: The development of genomic markers is described for Nile tilapia, Oreochromis niloticus, using the Diversity Arrays Technology (DArT) genotype-by-sequencing platform. A total of 13 215 single nucleotide polymorphism (SNP) markers and 12 490 silicoDArT (dominant) markers were identified from broodstock of two selective breeding programs [Genetically Improved Farmed Tilapia (GIFT) strain from Malaysia and the Abbassa strain from Egypt]. Over 10 000 SNPs were polymorphic in either strain, and 2985 and 3087 showed strain-specific polymorphisms for the GIFT and Abbassa strains respectively. We demonstrate the potential utility of these markers for rapid genomic screening and use in breeding programs.
PubDate: 2017-01-17T05:28:13.770155-05:
DOI: 10.1111/age.12536
- Authors: C. E. Lind; A. Kilian, J. A. H. Benzie
- Deep sequencing of a candidate region harboring the SOX9 gene for the
canine XX disorder of sex development
- Authors: J. Nowacka-Woszuk; I. Szczerbal, H. Pausch, S. Hundi, M. K. Hytönen, A. Grzemski, K. Flisikowski, H. Lohi, M. Switonski, M. Szydlowski
Abstract: A disorder of sex development (DSD) in dogs with female sex chromosomes (78, XX), a lack of the SRY gene and the presence of testes or ovotestes is commonly diagnosed in numerous breeds. The molecular background of DSD is not fully recognized but has been linked to the copy number variation in the region harboring the SOX9 gene. We applied a genome-wide association study and targeted next-generation sequencing techniques to compare DSD and normal female dogs. The genome-wide association study did not indicate a significant chromosome region. Targeted next-generation sequencing of a 1.5-Mb region on canine chromosome 9 harboring the SOX9 gene revealed two putatively DSD-associated copy number variations 355 kb upstream and 691 kb downstream of SOX9, four blocks of low polymorphism and two blocks of an elevated heterozygosity. An initial next-generation sequencing analysis showed an association with two SNPs, but validation in larger cohorts did not confirm this result. We identified a large homologous fragment (over 243.8 kb), named hfMAGI2, located upstream of SOX9, that overlaps a known copy number variation region. It shows a high sequence similarity with the 5′ flanking region of the MAGI2 gene located on canine chromosome 18 that encodes a protein involved in ovary formation during early embryonic development. Our study showed that the identified copy number variation region located upstream of the SOX9 gene contains potential regulatory sequences (long non-coding RNA and hfMAGI2) and led to the assumption that a multiplication of this element may alter expression of the SOX9 gene, triggering the DSD phenotype.
PubDate: 2017-01-17T05:28:07.456083-05:
DOI: 10.1111/age.12538
- Authors: J. Nowacka-Woszuk; I. Szczerbal, H. Pausch, S. Hundi, M. K. Hytönen, A. Grzemski, K. Flisikowski, H. Lohi, M. Switonski, M. Szydlowski
- Genetic diversity and patterns of population structure in Creole goats
from the Americas
- Authors: C. Ginja; L. T. Gama, A. Martínez, N. Sevane, I. Martin-Burriel, M. R. Lanari, M. A. Revidatti, J. A. Aranguren-Méndez, D. O. Bedotti, M. N. Ribeiro, P. Sponenberg, E. L. Aguirre, L. A. Alvarez-Franco, M. P. C. Menezes, E. Chacón, A. Galarza, N. Gómez-Urviola, O. R. Martínez-López, E. C. Pimenta-Filho, L. L. da Rocha, A. Stemmer, V. Landi, J. V. Delgado-Bermejo
Abstract: Biodiversity studies are more efficient when large numbers of breeds belonging to several countries are involved, as they allow for an in-depth analysis of the within- and between-breed components of genetic diversity. A set of 21 microsatellites was used to investigate the genetic composition of 24 Creole goat breeds (910 animals) from 10 countries to estimate levels of genetic variability, infer population structure and understand genetic relationships among populations across the American continent. Three commercial transboundary breeds were included in the analyses to investigate admixture with Creole goats. Overall, the genetic diversity of Creole populations (mean number of alleles = 5.82 ± 1.14, observed heterozygosity = 0.585 ± 0.074) was moderate and slightly lower than what was detected in other studies with breeds from other regions. The Bayesian clustering analysis without prior information on source populations identified 22 breed clusters. Three groups comprised more than one population, namely from Brazil (Azul and Graúna; Moxotó and Repartida) and Argentina (Long and shorthair Chilluda, Pampeana Colorada and Angora-type goat). Substructure was found in Criolla Paraguaya. When prior information on sample origin was considered, 92% of the individuals were assigned to the source population (threshold q ≥ 0.700). Creole breeds are well-differentiated entities (mean coefficient of genetic differentiation = 0.111 ± 0.048, with the exception of isolated island populations). Dilution from admixture with commercial transboundary breeds appears to be negligible. Significant levels of inbreeding were detected (inbreeding coefficient > 0 in most Creole goat populations, P
PubDate: 2017-01-17T05:27:41.557436-05:
DOI: 10.1111/age.12529
- Authors: C. Ginja; L. T. Gama, A. Martínez, N. Sevane, I. Martin-Burriel, M. R. Lanari, M. A. Revidatti, J. A. Aranguren-Méndez, D. O. Bedotti, M. N. Ribeiro, P. Sponenberg, E. L. Aguirre, L. A. Alvarez-Franco, M. P. C. Menezes, E. Chacón, A. Galarza, N. Gómez-Urviola, O. R. Martínez-López, E. C. Pimenta-Filho, L. L. da Rocha, A. Stemmer, V. Landi, J. V. Delgado-Bermejo
- On the origins and genetic diversity of South American chickens: one step
- Authors: A. Luzuriaga-Neira; G. Villacís-Rivas, F. Cueva-Castillo, G. Escudero-Sánchez, A. Ulloa-Nuñez, M. Rubilar-Quezada, R. Monteiro, M. R. Miller, A. Beja-Pereira
Abstract: Local chicken populations are a major source of food in the rural areas of South America. However, very little is known about their genetic composition and diversity. Here, we analyzed five populations from South America to investigate their maternal genetic origin and diversity, hoping to mitigate the lack of information on local chicken populations from this region. We also included three populations of chicken from the Iberian Peninsula and one from Easter Island, which are potential sources of the first chickens introduced in South America. The obtained sequencing data from South American chickens indicate the presence of four haplogroups (A, B, E and D) that can be further subdivided into nine sub-haplogroups. Of these, four (B1, D1a, E1a(b), E1b) were absent from local Iberian Peninsula chickens and one (D1a) was present only on Easter Island. The presence of the sub-haplogroups A1a(b) and E1a(b) in South America, previously only observed in Eastern Asia, and the significant population differentiation between Iberian Peninsula and South American populations, suggest a second maternal source of the extant genetic pool in South American chickens.
PubDate: 2017-01-17T05:27:34.051734-05:
DOI: 10.1111/age.12537
- Authors: A. Luzuriaga-Neira; G. Villacís-Rivas, F. Cueva-Castillo, G. Escudero-Sánchez, A. Ulloa-Nuñez, M. Rubilar-Quezada, R. Monteiro, M. R. Miller, A. Beja-Pereira
- Association analysis of KIT, MITF, and PAX3 variants with white markings
in Spanish horses
- Authors: S. Negro; F. Imsland, M. Valera, A. Molina, M. Solé, L. Andersson
Abstract: Several variants in the KIT, PAX3 and MITF genes have previously been associated with white markings in horses. In this study, we examined eight variants of these genes in 70 Menorca Purebred horses (PRMe, only black solid-coloured horses) and 70 Spanish Purebred horses (PRE, different coat colour patterns) that were scored for the extent of white markings. A maximum-likelihood chi-square test, logistic regression model and ridge regression analyses showed that a missense mutation (p.Arg682His) in KIT was associated with white facial markings (P
PubDate: 2017-01-13T06:45:39.820673-05:
DOI: 10.1111/age.12528
- Authors: S. Negro; F. Imsland, M. Valera, A. Molina, M. Solé, L. Andersson
- Issue Information
- First page: 253
PubDate: 2017-04-24T06:58:35.888922-05:
DOI: 10.1111/age.12499
- First page: 253


text:
If you want to create a dynamic and a single page website or a web application using such powerful framework so AngularJs is one of the option to fulfill your requirement. AngularJs helps to create the wonderful user interface. At InheritX Solutions, we have an amazing team of an experienced AngularJs developers who can give you the best in the industry. Hire AngularJs Developers from InheritX Solutions to give the best experience to users.
AngularJs is like an expansion for HTML. If you are looking forward to beautifying the effect of HTML, So you need to use an AngularJs such powerful JavaScript framework.
Above listed are few of the benefits of AngularJS which is only for your understanding that how much powerful this framework is.
InheritX Solutions is one of the leading brand in India nowadays who delivered more than 150+ mobile applications and 200+ web application and websites. Our delivery process is very transparent and accurate with following all the coding standards which make us proud. Our team can create any kind of web application using cutting-edge technology of AngularJs. By using our services in today's digital world will definitely make you smile.


text:
"It really is an unprecedented event," said Dr. Lyle Petersen, director of the division of vector-borne diseases at the Centers for Disease Control and Prevention. "You're dealing with children with a severe neurological disease they'll carry with them for the rest of their lives. That's a devastating outcome."
The concern is not just for babies. Zika has also been linked in Brazil to Guillain-Barre Syndrome, a neurological disorder that causes paralysis.
Also read: U.S. Says Some Demands on Qatar Will Be Difficult to Meet
Read more
Also read:
01:20 F.B.I. Agent Charged With Lying About Oregon Standoff Shooting8
20:15 US demands more security on international flights to US
17:45 Will the Supreme Court's Trinity decision lead to the spread of school voucher programs?13
19:12 Travel ban: Top 30 things Californians will miss about Texas23


text:
Definition of 'of concern'
of concern
If something is of concern to someone, they find it worrying and unsatisfactory.
Any injury to a child is a cause of great concern to us.
The survey's findings are a matter of great concern.
COBUILD Advanced English Dictionary. Copyright © HarperCollins Publishers
Example sentences containing 'of concern'
The police rarely seek such information unless the applicant has mentioned something of concern on their form.Times, Sunday Times (2010)
There is a great deal of concern in many parts of the judiciary about the number of failed asylum seekers.The Sun (2009) The Sun (2015)
It is thought to be something that is of concern only to the children, the childlike and the cranks.Times, Sunday Times (2008) Christianity Today (2000) Times, Sunday Times (2014)
Accordingly, he played with the freedom of a man for whom an iffy record in England does not carry a great deal of concern.Times, Sunday Times (2011)
Given that nearly half of all charity donations come from only 7 per cent of donors, there is a great deal of concern across charities.Times, Sunday Times (2012)
Definition of of concern from the Collins English Dictionary
Log in to comment on this word.
Cyber fraud
Why every generation is at risk
Read more
We take a look at the etymology behind the word 'election' in the run-up to the UK General Election in June
Language expert Ian Brookes looks at the word 'election' and its origins.
Read more
Join the Collins community
All the latest wordy news, linguistic insights, offers and competitions every month.
Read more


text:
Welcome to earthbaby boutique!
Can't make one of our workshops? Want to see a certain baby carrier or cloth diaper in person?
Baby carrier fittings: Try on our selection of baby carriers and get the assistance you deserve! $35.00 fee for a carrier fitting. Fee will be applied to any in-store purchase same day. *Baby Carriers are FINAL sale.
Cloth Diaper demo: Come in and let us show you everything you need to know and see to be a successful cloth diaperer! It can seem overwhelming online, but not to worry, we got your back! 45 minute education demo with a cloth educated earthbaby mama $35.00 fee. Fee will be applied to any in-store purchase same day.
Earth Baby Boutique is LA's premier cloth diaper boutique! We carry the largest selection of cloth diaper brands as well as babywearing, organic baby and maternity care, baby feeding products and unique eco-friendly toys. Earth baby was born from the idea that the internet shouldn't be the only place to find the cloth diapers your searching for, we know that every family has a preference and unique situation. Let us make finding the right cloth diaper solution easy with in store education & product selection assistance.
Here at Earth Baby our greatest mission is to create a caring judgment free place where people can comfortably come and learn, share and buy the things they need to create a green and happy lifestyle for their families. For us going green was a path we just found our selves on one day. What we found was a huge eye opening but overwhelming world of products and information. We want to help make your path simple and easy. Regardless of whether or not you are the crunchiest of granola greens, or a newbie to the green scene, we are here to help! No question is to small, and every little step makes an impact.
Owner Renee Kennedy Powers started using cloth diapers in 2013 when her son Tannen was born highly allergic to disposable diapers. After learning about the toxic substances found in disposable diapers and the environmental impact they leave on our planet cloth diapering became more then just a necessity, it became a passion! Renee wants to share her passion with others by spreading the word about all of the great benefits of using cloth diapers and making it accessible and practical for everyone.
Come and visit us at:
Earth Baby Boutique
13454 Ventura Blvd.
Sherman Oaks, CA 91423
Our hours are:
Tuesday-Wednesday-Thursday & Saturday 11am to 6pm
Friday 11am-7:30pm
Sunday 12pm to 4pm
Want more information? Have a question? Call us! We are here to help.
(818) 917-6193
Love, The Powers Family


text:
Availability: Many in stock
The days of the $30 LED bulb are over.
As demand has increased and manufacturing processes have become more streamlined, costs have plummeted. Additionally, utility company rebates have driven the price of many household replacements to below $10; in some regions they cost half that.
Sure, that's a long way from the 50-cent incandescent, but consider this - LED bulbs consume one-sixth the energy of an equivalent incandescent and last up to 25 times longer.
Replacing a 60-watt incandescent with an LED equivalent will save you $130 in energy costs over the new bulb's lifetime. The average American household could slash $150 from its annual energy bill by replacing all incandescent with LED bulbs.
Reputable Reference - http://www.popularmechanics.com/home/how-to/a10104/everything-you-need-to-know-about-led-light-bulbs-16542040/
There is still considerable question on LED scope quality as compared with other technology.
The future is here.
These are standard, RoHS and CE certified light bulbs that are guaranteed to last over 5yrs! That is not a separate guarantee, that is the expected attribute of the product, absolutely amazing isn't it. Mostly because there is not a great deal of power used and so it does not undergo the destruction process from this energy.
There is no doubt about best buy and value. Common sense prevails and I suggest using the best lighting for your needs.
Sometimes you want a toilet light that is warm because the loo is outside, that is rational.
I would suggest installing one of these LED lights in a place where the light is most often needed for long duration or cannot be controlled, like with security lights.
2-3 x 9W LED globes in a games room to give it the lighting it needs to play your favourite games.
LED lighting is particularly effective when reflected by light colors, this can be used to an advantage or objective. Incandescent lights could not be focused easily at all, now that has changed, yes BIG WINK my friends.
- Base socket type: E27, B22, E14
- Input Voltage: AC220V - 240V
- Power: 3W / 7W / 9W /12W (candle, 40W, 60W and 75W incandescent equivalent)
- Type: LED bulb [Non-dimmable]
- Styles: 3
- Beam Angle: 140 degree
- Average Life: >50,000hours
- Colour: Warm White (3000- 3500K); Cool White (6000-6500K)
- Luminous Intensity: 7W(595-640lm) 9W(765-820lm) 12W(1200-1350lm)
- Storage Temperature: -40°~+60 DegC
Change-over adaptors available
- Fittings: E27M-B22F, B22M-E27F, E14M-E27F, E14M-B22F
- Certification: CE & ROHS
- Energy efficient: Saves more than 90% energy when compared with traditional halogen lamp.
- High brightness: Brightness degradation is less than 1% per 1,000 hours.
- Long lifespan: 100,000 hours (17 years at 8 hours a day), longer than most of lamps in LED market.
- Advanced technology: safer, ultra-bright, amazing performance.
- Patent heat dissipation design, long working time is guaranteed
- 90% saving on the electricity bills, and greatly reducing maintenance cost.
- Instant start, no flashing.
- No UV/IR Radiation, and no heat radiation.
A. Outstanding Energy and Electricity Bill Savings
Compared to normal halogen lamp, LED bulb can save 85% energy and electrical consumption. When a LED bulb is turned on, the power meter almost doesn't move.
B. Long Life Time of 50,000 Hours
10 times longer than incandescent and halogen bulb and directly reduce the bill.
C. Low Heat Production
LED bulb generate very low heat, because 80% of the electrical energy converts to light instead of heat. On the contrary, the light conversion of halogen lamps is very low.
D. Top-tier Quality
All our products are CE, RoHS and WEEE Approved. Please note these bulbs are the best quality on the market. Most other LED bulbs brightness degradation fast and bulbs shade after only a few hours use. Welcome Comparison!!!
Our products come from many very far away places under many different ways of travel.
We have organized our Delivery System for a dedicated staff member to personally process your order for it's Shipping costs, if any are applicable.
We then send you a Payment Confirmation for the total of your order which now includes shipping at the best rates in Australia.
Of course you can select a Shipping Method from our List when finalizing your Bag but if any doubt at all, please select the Civilstock Office option.
Please note: ALL Orders are manually processed always. So, if a shipping option is added to the Bag, there is still no charge, NOT until a payment confirmation is sent by our staff member who has manually checked all parts of your order and is completely satisfied that we are going to deliver your product(s) as inexpensively as possible, and has fully applied all policies of supplier shipping credits to your order.
At Civilstock, you will always have the opportunity to decide for yourself.


text:
Register to Top Craft Sites - Rank your site now
Hannahbella Cards & Lovehuggles
Handmade cards, wedding stationery, gifts and gift wrap. Mini scrapbooks all with a little lovehuggling thrown in
Username: Gerribell
URL: http://hannahbella.co.uk
Category: Blogs
Join date : 2011-04-05
Average Rating: 4 / 5
Number of Ratings: 3
Rate and Review This Site
Similar Sites
Stained Glass Patterns | My Stained Glass Blog - My blog about stained glass. Free stained glass patterns, stained glass construction and art glass design techniques.
from crayons to Copics - I love to stamp and make cards. I now have over 1000 cards on my site. I am on many different design teams. Please stop by my blog for ideas and let me know you visited.
A Crafting Journey - **Craft Stamper Blog of the month** DT member for Craft Stamper magazine, Zutter, L.B Crafts and Simon Says Stamp & Show! Certified Ranger Educator sharing hints, tips and general all round inkiness!
Sugar Nellie Stamps - From a small ruber stamp company in Scotland comes the sweetest stamps, a dream to ink and a pleasure to colour. News, samples and candy.
Handmade by Tigerlily - Handmade by Tigerlily is the home for everything handmade. Tigerlily's aim is to source and use environmentally friendly products to create stunning one off items from greetings cards to felted bags
Top Craft Sites - Powered by Top Craft Sites - Sitemap
Load Time: 0.01337 | SQL Queries: 11 | Active Members: 606


text:
|Story Views
|Last Hour:
|Last 24 Hours:
We've got two stories coming out today that pretty much confirm what the pessimists among us always knew: The GOP isn't going to repeal Obamacare, and it's entirely likely they never were serious about it.
The first bit is from TheBlaze, and it focuses on one Republican Senator in particular – Lisa Murkowski.
During Murkowski's annual address to the Alaska legislature Wednesday evening, she addressed concerns about Republicans' plan to dismantle the controversial Affordable Care Act and promised to vote according to her state's wishes when it comes to Medicaid and Planned Parenthood.
"Here in Alaska, some 27,000 Alaskans — about 28,000 actually — now have coverage for the first time," Murkowski said. "Which means that they have access to care for the first time. While I clearly have concerns about the expansion's long-term costs, it has strengthened our native health system and reduced the number of uninsured that are coming into our emergency rooms."
She added, "So as long as this legislature wants to keep the expansion, Alaska should have that option. So I will not vote to repeal it."
Murkowski, if you will recall, also broke rank with Republicans on confirming Betsy DeVos, and has taken money from liberal groups (like teachers' unions).
However, one Senator's words aren't enough. There is a man who exemplifies everything that was (and still is) wrong with the GOP establishment who has more or less confirmed all this with his own words. That man is former House Speaker John Boehner.
"[Congressional Republicans are] going to fix Obamacare – I shouldn't call it repeal-and-replace, because it's not going to happen," he said.
Boehner's comments come as Republican lawmakers across the country are facing angry constituents at town halls worried that Obamacare will be yanked away without a suitable replacement.
President Donald Trump has said in recent days that he will release a plan by early to mid-March on how the administration plans to move forward on a repeal-and-replace plan.
On Thursday, Boehner said the talk in November about lightning-fast passage of a new health care framework was wildly optimistic.
"I started laughing," he said. "Republicans never ever agree on health care."
"Most of the framework of the Affordable Care Act … that's going to be there," Boehner concluded.
Boehner would know, because this is exactly how it's done in Washington D.C., and specifically how it's done in the Republican Party.
The GOP has spent YEARS taking money from its base and saying how Obamacare was the worst thing in the world and how, by God, if we gave them the House they'd fix this mess… and then if we gave them the Senate… and then if we gave them the White House.
And it's not going to happen.
See, it's something a lot of us have known forever: Once a new entitlement is enacted, it's nigh-impossible to undo. There are ways to do it, but there is simply no one with the willpower to push the full repeal through.
Instead, what we're going to get is some half-assed mix-and-match effort to patch things up, which will only go to destabilize the insurance market even further. And, this time, it's not just a Democratic Party law. It'll be bipartisan.
Just the way they like it.
The post SHOCKER: Yeah, the GOP Isn't Going to Repeal Obamacare. appeared first on RedState.
FBI Agent Indicted for False Statements in LaVoy Finicum Shooting Death
What Really Happened in Waco, Texas - Twin Peaks Biker 'Shootout' (Video)
The US Is Becoming A 3rd World Nation As The Economy Breaks Down | Crash to Happen in August? (Videos) X22 Report
The Federal Reserve Is a Saboteur, the "Experts" Are Oblivious
MSNBCs Rachel Maddow Goes Beyond Full Libtard on Why Democrats Keep Losing!
David Icke: The Secret Headquarters of the Illuminati (Video)
Payday! Sarah Palin Sues New York Times for Defamation (Video)
The Housing Bubble Hangover Cure
Sanders: FBI investigation of his wife and him. 'It's Trump's fault'
Empires collapse. Then what?
CNN HIts News Low By Using Sesame Street's Elmo To Attack President Trump's Travel Ban
European Parliament Members Express Concern over Human Rights in Iran and Support for Democratic Opposition
North Carolina Man Sentenced to Life in Prison for Attempting to Commit an Act of Terrorism Transcending National Boundaries
Fallout From CNN Anti-Trump Fake News Story Now Reaching As High As Network President Jeff Zucker
Proddatur News
Tamil Nadu boy designs world's 'smallest' satellite for NASA
The best mood lifter in the world!
iPhones And Siri Are Getting Ready To Take Over By Integrating Into Every Aspect Of Our Daily Life
Japanese Government Begins Preparing Its People On What To Do In Event Of A Nuclear Missile Strike
Syrian warns Israel of "grave consequences" for continuing attacks
Mankind Did Not Evolve from a Previous Life Form
Soros Group Plans to 'Desecrate Graves' at Battle of Gettysburg (Video)
Everything Is A Lie! Jeff Berwick Unleashed!
World Wide Earthquake Imminent!
Citizen Journalist Exposes CNN Corruption, Conspiracy to Oust Trump +Video
Heads Up NorCal! Mega Quake Precursors? Sierra Shakes With More Than A Dozen Earthquakes Possible M6+ in Next 3 Days (Videos)
Beautiful! This Is How the Free Market Takes Care of Arrogant Punks
Yellowstone Supervolcano Earthquake Swarm Reaches 878 Quakes In Just Two Weeks (Video)
Infowars Nightly News - Tuesday (6-27-17) Terrence Williams
The Materially Insane on the Runaway Train to Krazy Town.


text: Breadcrumb Home / Dictionary Meaning of reest Find definitions for: reest Pronunciation: (rēst), [key] to cure, smoke, or dry (meat or fish). reest Pronunciation: (rēst), [key] (of a horse) to stop or refuse to go; balk. Random House Unabridged Dictionary, Copyright © 1997, by Random House, Inc., on Infoplease. Related ContentDaily Word Quiz: opprobriumAnalogy of the Day: Today's AnalogySpelling Bee: Today's Spelling BeeKids' Word Quiz: preparatoryCrossword: Crossword Puzzle GuideFrequently Misspelled WordsFrequently Mispronounced WordsEasily Confused WordsWriting & Language Related Content Daily Word Quiz: expand Analogy of the Day: Today's Analogy Spelling Bee: Today's Spelling Bee Frequently Misspelled Words Frequently Mispronounced Words Easily Confused Words Writing & Language


text:
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ ReadersThis Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Objective: To identify factors indicating disease severity in patients with thoracolumbar tuberculosis requiring surgical intervention.
Design: Medical charts of patients who underwent surgery for thoracic and lumbar spinal tuberculosis from 1990-2005 were reviewed. Patients with different levels of disease were compared in terms of neurological deficits, duration of symptoms, previous antituberculous therapy, nutritional status and associated co-morbids.
Results: Ninety-three patients aged 7-77 years (mean age 40 years) were included. Thoracic spine was involved in 80% of operated patients, and lumbar spine in 20%. Severe neurological impairment (Frankel A to C) was present in 68% of patients with thoracic disease, as compared to 5% with lumbar disease (p<0.05). Postoperatively, complete neurological recovery occurred in 65% with thoracic versus 100% with lumbar disease (p<0.05). Based on the disease location and pertinent elements in clinical history, physical signs, radiographic and biochemical features, a scoring system was developed.
Conclusion: In endemic areas with limited resources, strategies for cost-effective care are needed. By objectively outlining the treatment approach, the judicious use of surgery offers hope for enormous cost savings in countries endemic for tuberculosis, averting complications from disease progression.
To read the full article Peer-reviewed Article PDF
Author(s): Shahryar Noordin Salim Allana Tashfeen Ahmad Zafar Sajjad KhabirAhmad Syed A Enam Riaz H Lakdawala
Thoracolumbar tuberculosis, spondylodiscitis, scoring system, disease severity, surgery


text:
How to write my name in japanese on facebook
How to write my name in japanese on facebook Rated stars, based on customer reviews From $ per page Available! Order now!Eamcet practice papers online renewable energy phd thesis. Who can write my resume costs and budgets essay critical essay merchant new venice lennie small character analysis essay what is persuasive writing ks2 how to write an application letter for employment as a driver best way to start a compare college application essay service harvard and contrast essay how how to write my name in japanese on facebook to arrange bibliography in buy a dissertation online advertising alphabetical order in word 2007. Essay writing service turnitin. Marketing strategies essay custom write my essay write my name in stylish fonts for facebook. How to write a book reference john wood community college writing your research paper i do my homework last minute dissertation sebastian grasreiner help writing common app essay anti semite and jew essay. What can i do my reflective essay on economic dissertation help online professional resume writing services kenya.
Buy research proposal papersMacbeth essay help principles of marketing essay questions and answers example of a descriptive essay about a picture top college creative writing programs eight steps in critical thinking long essay on earthquake in english research essays on gay marriage chinese essay writing service essay writting format, essay helper words best writing service websites government jobs application format best way to end college essay intellectual property thesis topics. Phd thesis transitional justice. Help with powerpoint presentation help with writing a dissertation 3 weeks order toilet paper online communication 21st century essay. Help with paperwork at home. Cover letter professional writing service course creative writing essay sites in hindi sample resume for sales advisor education essays ielts essay how to write my name in japanese on facebook line graph architectural thesis on heritage conservation writing a cover letter for medical school analysis phase of research paper research papers consumer finance. Can you buy reviews on amazon do my homework french translation order paper online uk phd dissertation writing services usa jobs resume writing service. Resume writing services hobart. International business reports. Persuasive speeches for sale appendix in thesis inspector calls gcse coursework. Financial business plan template short speech about air pollution essay on david milgaard admission papers for sale paperpk, science homework help 8th grade business homework help online. Online help with social studies homework.
Pa pro life essay contestMacbeth essay help english essay college students help with powerpoint best place to buy research paper presentation, education essays ielts essay sites in hindi. Principles of marketing essay questions and answers, resume writing services hobart order paper online uk. Essay writting format... Biology unit 5 essay help financial business plan template educational objectives for resume business homework write my essay usa help online phd thesis transitional justice order toilet paper online appendix in thesis essay helper i write college essays service words. Eight steps in critical thinking. Help with paperwork at home research essays on gay marriage phd dissertation writing online essay service services international business reports writing a cover letter for medical school essay writers and homework usa jobs resume writing service essay line graph do my homework french translation.
Pipefitter cover letterIntellectual property thesis topics architectural thesis on heritage conservation. Example of a descriptive essay about a picture short speech about air pollution. Can you buy reviews on amazon best way to end college essay inspector calls gcse coursework online help with social studies homework. Course creative writing best writing service websites help with paperwork at home chinese essay writing service government jobs application format essay on david milgaard top college creative writing programs. Communication 21st century essay admission papers for sale paperpk science homework help 8th grade cover letter professional writing service persuasive speeches for sale.
Related posts:
- Last minute homework help
- Help with assignments sydney
- Assignment help in melbourne
- Write a research paper for me cheap
- Sample resume for sales advisor
- Buy dissertation paper


text:
Low Rate Card Offers FAQs
An Ebates credit card doesn't have any 0% intro APR, so the interest will start accruing right away, and if you don't pay off the balance within the grace period, you may face quite a sum to pay. The interest on the card is 15,74%. If you are averse to paying such a large interest, […]Continue »
Although the NavyFCU offers multiple credit cards, they only have one card available that would be the most useful in your situation. That would be the Navy Federal Credit Union Platinum card. The platinum card offers the best interest rate out of all their cards, with a Variable APR of 8.74% to 18.0%. When making […]Continue »
You are smart to consider whether this type of in-store financing plan was the best way to fund your purchase. For your next major appliance, consider applying for a credit card that offers a 0% APR on purchases for the first year, followed by a low ongoing APR. Having a low-interest credit card is a […]Continue »
How much APR will I pay if I apply for a HEB credit card?
The APR, or the annual percentage rate, is fixed on the HEB credit cards. It depends on the type of the card you are applying for. Currently on the hebfcu site you can find three credit cards: Regular MasterCard, Gold MasterCard and Platinum MasterCard. The Gold and Platinum credit cards are more suitable for customers […]Continue »
How does the BrandsMart payment plan work?
This is an in-store financing option that allows you to pay off your purchase in anywhere from 12 to 36 months. Here's how it works: when you make a purchase and opt-in to the payment plan, you'll pay a certain amount each month, without paying any interest. If you pay off the entire amount in […]Continue »
To take advantage of any of the financing plans that are available from Discount Tire Center, you must have the Discount-Tire Center credit card. Once you have their card, you need to spend at least $199 and you must use your Discount Tire card to make your purchase. If your purchase qualifies, it will be […]Continue »
Ever since Ikea dropped their credit card in 2011, there hasn't been any way to directly finance a large purchase through Ikea. Although Ikea can't help you finance your purchases, it doesn't mean you are entirely out of options. There is currently a great amount of general credit cards available to people who are in […]Continue »
Credit Card FAQ Categories
- Bad Credit Cards
- Balance Transfer Cards
- Best Credit Cards
- Business Credit Cards
- Credit Card Banks
- Credit Card Fees
- Credit Card Rewards
- Fair Credit Cards
- Foreign Credit Card Offers
- Instant Credit Card Offers
- Low Rate Card Offers
- Mastercard Credit Cards
- No Credit Cards
- Prepaid & Debit Cards
- Secured Credit Cards
- Student Credit Cards
- Travel Credit Cards
- Uncategorized
- Visa Credit Cards


text:
Biological production of optically active muconolactones by Rhodococcus rhodochrous
- Received:
- Accepted:
DOI: 10.1007/s002530100668
- Cite this article as:
- Cha, CJ. Appl Microbiol Biotechnol (2001) 56: 453. doi:10.1007/s002530100668
- Citations
- 30 Downloads
Optically active (-)-3-methylmuconolactone was biologically produced using a mutant strain of Rhodococcus rhodochrous N75 that is capable of metabolizing 4-methylcatechol via a modified ortho-cleavage pathway. The mutant strain (CJ30) was prepared by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine and found to be blocked in the degradation of 3-methylmuconolactone. Cells of the mutant CJ30, which had been previously grown on yeast extract and induced with p-toluate, transformed p-toluate (11.5 mM) to optically active (-)-3-methylmuconolactone with a yield of 53%. The structure of 3-methylmuconolactone was confirmed by NMR spectroscopy and mass spectrometry. Cell-free extracts of R. rhodochrous N75 also transformed a range of 4-alkylcatechols, such as 4-ethylcatechol, 4-iso-propylcatechol, and 4-tert-butylcatechol, to the corresponding 4-alkyl-substituted muconolactones.


text:
Press Releases
StreamSets Secures $20 Million in Series B Funding to Conquer Dataflow Chaos
Funding Fuels Industry's First Data Operations Platform That Delivers 'Air Traffic Control' Across Cloud, Batch and Streaming Data Architectures May 31, 2017SAN FRANCISCO – May 31, 2017 – StreamSets Inc., provider of the industry's first enterprise data operations platform, today announced that it has raised $20 million in Series B funding. Leading venture capital firms Accel
StreamSets Named a 2017 "Cool Vendor" by Gartner
Company Named as an Innovative Vendor in Data Management May 2, 2017SAN FRANCISCO – May 1, 2017 – StreamSets Inc., a provider of an innovative platform for managing data-in-motion, today announced it has been recognized in Gartner, Inc.'s "Cool Vendors in Data Management, 2017" report published on April 28, 2017. The four vendors named in
Fortune Ranks StreamSets Among 25 Best Workplaces in the Bay Area
Distinction Underscores Company's Commitment to a Vibrant Culture and Diverse Workforce April 26, 2017SAN FRANCISCO – April 26, 2017 – StreamSets Inc., a provider of an innovative platform for managing data-in-motion, has been named to Fortune's ranking of "Best 25 Small and Medium Workplaces in the Bay Area." StreamSets was ranked No. 14 on the
StreamSets Ranked Third in List of 2017 Best Places to Work
Annual List From SF Business Times and Silicon Valley Business Journal Lauds Select Bay Area Small Businesses April 19, 2017SAN FRANCISCO — April 19, 2017 —StreamSets Inc., a provider of an innovative platform for managing data-in-motion, has been named to the list of 2017 Bay Area Best Places to Work, an awards program presented by
StreamSets Enables Data Lake in the Cloud with Microsoft Azure Integration
StreamSets Data Collector Now Available for Azure Customers, Helping Enterprises Accelerate Execution of Their Cloud Strategies March 22, 2017SAN FRANCISCO — March 22, 2017 — StreamSets Inc., a provider of innovative data-in-motion middleware, today announced integration of its award-winning StreamSets Data Collector™ open source software for building any-to-any dataflows with Microsoft Azure. The integration enables
In the News
Silicon Angle
Data operations startup StreamSets raises $20M in funding
"StreamSets Inc. wants to help organizations work through the checklist more easily. For its efforts, the startup has received $20 million in funding announced today from venture capital giants Accel Partners, Battery Ventures and New Enterprise Associates. The cash will go toward developing new features for its flagship "data operations" platform. "
Venture Beat
StreamSets raises $20 million to help companies manage big data
"StreamSets, which provides software to manage various sets of data from a single control panel, today announced funding of $20 million in a round co-led by New Enterprise Associates (NEA) and Battery Ventures. Existing investor Accel also joined."
The 25 Best Small and Medium-Size Workplaces in the Bay Area
"Despite our own desire to get more done and hit every deadline, management consistently reminds us to take care of ourselves, and our families—to go home and take time off to have fun. They do their best to create a fun environment at the office, with regular gatherings and celebrations. Our families are encouraged to join in, too, so we are one happy family. Most companies give lip service to work-life balance, but our leaders mean it."
How to Create a Custom StreamSets Origin That Supports Parallel Execution
"Origins in StreamSets Data Collector represent the source of data for a pipeline. Learn how to create a custom StreamSets origin that can support parallel execution."
"A recent StreamSets user community survey provides key findings on the prevalence of streaming data and mix of traditional and Big Data applications."