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Multinational corporations are lobbying the U.N. behind closed doors to keep tax avoidance off the list of targets in the Sustainable Development Goals, say advocates of global tax reform. Many experts cite tax avoidance by corporations and wealthy individuals as a major driver of inequality and poverty worldwide. The United Nations' Sustainable Development Goals (SDGs), established by international consensus as a series of goals for reducing poverty and inequity, included reducing tax avoidance and evasion.
World Politics
Republican attempts to indict Hillary Clinton did not end with the election and ongoing inquiries now put a Bangladeshi Nobel Peace Prize winner squarely in the crosshairs. Muhammad Yunus, and anti-poverty advocate of microfinance, is under political attack at home that is now fueled by the GOP.
Humanitarian needs are growing worldwide and international donors are not keeping up. So far, only one-quarter of the money requested for 2017 is available to respond to crises ranging from Syrian refugees to the more than 20 million people at risk of famine. More money is needed due to deteriorating conditions in conflict regions and the recent rapid growth of violence in the Kasai province in the Democratic Republic of the Congo.
The United Nations is urging countries around the Caribbean to view the recent surge in refugees, from near and far, as a long-term benefit and not just a short-term challenge. More than 5,000 people came to various Caribbean nations last year seeking asylum, representing a 257 percent increase in the number of asylum seekers between mid-2015 and mid-2016 for the region.
As the battle continues between Islamic State-affiliated militants and the Philippine military in the southern city of Marawi, humanitarian agencies are scrambling to care for the hundreds of thousands of evacuees who have fled the devastation. An estimated 300 to 500 civilians remain trapped in the city, held hostage as 'human shields' for the rebels.
Human rights experts are warning about negative impacts from U.S. President Donald Trump's plan to revive some of the trade and travel restrictions with Cuba that former President Barack Obama had relaxed in an effort to improve relations between the two countries. Many had said the sanctions against Cuba had accomplished little, other than to undermine the economic opportunities of Cubans.
Despite widespread opposition, Japan has passed a controversial counter-terrorism law that targets the planning stages of crimes. And you thought the Philip K. Dick book and movie Minority Report about a futuristic law enforcement agency of thought police was just science fiction!
Canada has decided to shift its foreign aid spending so that it emphasizes empowering women and girls. By setting a goal of 15 percent of aid spending on gender equality, Canada will become the single largest contributor in bilateral funding to women's rights organizations.
Germany wants to start a new global emergency relief fund just as the U.S. announces its intention to leave the United Nation's Green Climate Fund. The announcements come roughly a week apart and show how diverging priorities for some of the world's largest economies may reshape the humanitarian system.
If you are an American, you might be excused for thinking the world is less peaceful lately. But in fact, overall, the planet is a bit more peaceful than it was last year. Not so much for the U.S., however, or for regions embroiled in war and civil conflict. That's the assessment of the new Global Peace Index in its annual report based on 2016 statistics.

subset: commoncrawl


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Maybe Tim Cook should stop speaking...
On Wednesday Tim Cook (Apple CEO) spoke for the 6th time in the last 5 months publically. Why does this matter you ask? Well, unforutnately for Apple stock holders, each of these last 6 times Tim Cook has spoken publically Apple's stock has dropped. To make things even worse, over the last 5 months these drops have added up to be quite significant which has seen Apple's stock drop from the 600+ range to the mid 400 dollar range. I'm sure most Apple stock holders would be very happy if Tim Cook took a vow of silence. Huffington Post has more details.
On Wednesday afternoon, Apple CEO Tim Cook addressed investors and the media at the company's annual shareholders meeting. It was the sixth time in the last five months that Cook has made something like a public appearance; and it is also the sixth time in the past five months that Apple's stock (AAPL) has closed down after Cook appeared.
Consider this: The last six times that Cook has put himself out there, Apple's stock declined afterwards. It's a streak that dates back to October 2012, when Cook introduced the iPad mini, and it is a trend that has gone unbroken for about five months now: When Cook appears, AAPL goes down


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From shotgun libraries used for the genomic sequencing of the phytopathogenic bacterium Xanthomonas axonopodis pv. citri (XAC), clones that were representative of the largest possible number of coding sequences (CDSs) were selected to create a DNA microarray platform on glass slides (XACarray). The creation of the XACarray allowed for the establishment of a tool that is capable of providing data for the analysis of global genome expression in this organism.
The inserts from the selected clones were amplified by PCR with the universal oligonucleotide primers M13R and M13F. The obtained products were purified and fixed in duplicate on glass slides specific for use in DNA microarrays. The number of spots on the microarray totaled 6,144 and included 768 positive controls and 624 negative controls per slide. Validation of the platform was performed through hybridization of total DNA probes from XAC labeled with different fluorophores, Cy3 and Cy5. In this validation assay, 86% of all PCR products fixed on the glass slides were confirmed to present a hybridization signal greater than twice the standard deviation of the deviation of the global median signal-to-noise ration.
Our validation of the XACArray platform using DNA-DNA hybridization revealed that it can be used to evaluate the expression of 2,365 individual CDSs from all major functional categories, which corresponds to 52.7% of the annotated CDSs of the XAC genome. As a proof of concept, we used this platform in a previously work to verify the absence of genomic regions that could not be detected by sequencing in related strains of Xanthomonas.
Citrus canker, or cancrosis, is a disease that affects most species of the Citrus genus [1]. Its symptoms can be observed on leaves, fruits or branches and are characterized by small, pointed and spongy pustules that are surrounded by a chlorotic halo on both sides of the leaf. This halo tends to spread quickly through the tissue, increasing its diameter and becoming brownish or darker in color with a rough and salient appearance [2]. The causal agent of cancrosis is the bacterium Xanthomonas axonopodis pv. citri (XAC) [3], more recently renamed as Xanthomonas citri subsp. citri [4,5] whose genome was completely sequenced in 2002 [6] and compared with others organisms [6-8]. Data from this sequencing project revealed that the XAC genome contains a circular chromosome of 5.2 Mbp and two plasmids (33 and 64 Kbp), containing a total of 4,489 coding regions [6].
To perform the complete sequencing of this phytopathogen, shotgun libraries containing 46,462 clones were created and distributed over approximately 500 96-well plates, representing 98% of the genome, with an estimated coverage of seven times its size [6]. The remaining 2% of the genome was sequenced using a cosmid library.
Upon completion of the sequencing project, the clones were stored with the expectation that they would be useful for future functional genomics studies. We here explore one of the possible applications of this library, namely the use of these clones to build a DNA microarray platform for use in studies of gene expression and genotyping [9]. Building on the data provided by the XAC genome project, this tool will enable the exploration of the physiological and biochemical machinery of this organism in distinct environmental situations, both in vitro and in vivo, thus providing clues to the working of Xanthomonas metabolism and the mechanisms of infection and disease.
The clones in the XAC sequencing libraries were generated from total bacterial DNA (chromosomal DNA + plasmid DNA) using shotgun methods [10] and cloning into the pUC18 vector. The number of clones totaled 46,462 with inserts ranging in size from 400 to 5,200 bp (Figure 1AB) [6]. All of these clones were placed in 496 96-well plates, and stored at -80°C. In order to represent the CDSs in the XACarray, clones that included the largest fragments (0.4 to 3 Kbp) of each CDSs of interest were selected. However, not all of the CDSs were represented by clones that included a single open-reading frame (ORF). Actually, many CDSs were represented by clones that possessed inserts with parts of other CDSs or even whole CDSs. Thus, the selected clones were divided into four possible overlap types, considering the sequence segment of interest in relation to the insert as a whole: I) the CDS is completely represented within the insert and is surrounded on both sides by intergenic regions and/or other CDSs; II) the insert is an internal fragment of the corresponding CDS; III) part of the interested CDS flanking the left margin of the insert, or IV) part of the interested CDS flanking the right margin of the insert (Figure (Figure1C).1C). Details of the relationship between the insert and CDS of interest are described in the results and are summarized in Table Table11.
The selected clone inserts were amplified through PCR after DNA extraction by the boiling preparation technique [11]. The amplification reactions were performed with an initial denaturation at 95°C for 5 minutes, followed by 40 denaturing cycles at 95°C for 45 seconds, annealing at 50°C for 30 seconds and extension at 72°C for 1 minute in a final volume of 50 μL of amplification solution containing 1.0 μL boiling prep product, 1 U Taq DNA Polymerase™ (Invitrogen), 40 mM dNTPs (Invitrogen) and 10 pmols of each universal oligonucleotide M13 (forward 5'-GTTTTCCCAGTCACGAC-3' and reverse 5'-CAGGAAACAGCTATGAC-3'). In an attempt to increase the amount of the generated product, 1.0 μL of the amplification reaction was added to new amplification solution with the same volume and concentration of reagents and submitted to the amplification protocol described above.
The amplification products were purified with Multiscreen Millipore™ (cat. # MAFB NOB 50) filters plates and diluted in 50 μL of 10 mM Tris pH 8.0 directly into 96-well plates. The purification products were then manually combined in 8 384-well plates; one of these plates contained the hybridization controls (ScoreCard [12]).
In all steps, the PCR products were evaluated by electrophoresis in 1% agarose gels stained with ethidium bromide [13]. Amplicon size and quantity were estimated by comparison to a molecular weight standard that was created specifically for this purpose (Figure (Figure2A).2A). This standard contained three DNA fragments of 1,697, 689 and 297 bp, respectively, with pre-determined concentrations. The standard was produced by cleavage of the pUC19 plasmid with the restriction enzymes HindIII, NdeI and ScaI, respectively, and was purified using a cesium chloride density gradient column [14]. This strategy allowed for comparative visual analysis of the sample concentrations in the gels, eliminating the need for individual quantification of each observed product.
To ensure that each amplified PCR product was in fact the clone selected for each CDS, an end of the insert in each of the clones was sequenced using the universal oligonucleotide primer M13R and the BigDye terminator v3™ kit (Applied Biosystems). These sequences were then compared to the database of sequencing reads and the XAC genome using the BLASTn algorithm [15].
Equal volumes of DMSO were added to the purified PCR products in 384-well plates so that the final concentration was approximately 200 to 400 fmol/μL of DNA [16]. Afterwards, DNA was deposited onto Type 7 mirrored glass slides (Amersham Biosciences/GE Healthcare) using a robot (Generation III Microarrays Spotter™ - Amersham Biosciences/GE Healthcare). This robot allowed for the deposition of up to 4,608 DNA samples that were organized into 12 subsets (subarrays) of 384 spots (12 × 32) (Figure (Figure2A).2A). The set of 3,072 probes, which corresponded to the products on the 384-well plates and included positive and negative controls, was placed in duplicate in the two longitudinal halves of the slide. The duplicates were called set A and set B, respectively, and constituted a technical replica on each slide (Figure (Figure2A).2A). After DNA deposition, the slides were submitted to 50 mJ UV light, which promoted the fixation of the DNA onto the support. The slides were stored in desiccators (relative moisture ~5%), ready to be used for any experimental purpose.
The XAC 306 isolate was extracted from Citrus limonia L. Osbeck (rangpur lime)-infected leaves and stored in 30% glycerol at room temperature until use. The strain was sowed to reactivate its growth in NA solid medium (3 g/L of meat extract, 5/L g of peptone, 5 g/L of sodium chloride, 15 g/L of agar) and was incubated at 28°C for 48 hours. Samples of the reactivated strain were minced in 12 mL of NA liquid medium in 25-mL Falcon tubes and maintained under gentle agitation at 28°C for 48 hours. Appropriated antibiotic was used when necessary. After this period, the samples were centrifuged for 30 minutes at 3,000 g at room temperature. The supernatant was discarded, and 12.5 mL of wash buffer (10 mM Tris-HCl, pH 8.8, 3.0 mM KCl, 1.25 mM NaCl) was added to the bacterial pellet. The samples were shaken vigorously using a vortex and then centrifuged for 15 minutes at 3,000 g at room temperature. The bacterial pellet was resuspended again, now using 10 mL of D buffer (25 mM sodium citrate, pH 7.0, 5.0 g/L sarkosyl, 4 M guanidine isothiocyanate), and incubated in a 65°C water bath for 1 hour to promote cell lysis. After the lysis step, 5.0 mL of P buffer (667 mM Tris-HCl, 833 mM NaCl, 83 mM EDTA, pH 8.0) was added. The solution was shaken by inversion and centrifuged for 30 minutes at 3,000 g at room temperature. Aliquots (1.0 mL) of the supernatant were transferred to tubes with a 2.0 mL capacity, 1.0 mL of isopropanol was added and the samples were kept at -20°C. The samples were then homogenized by inversion and centrifuged for 45 minutes at 3,000 g at 4°C. The DNA pellet was resuspended, washed twice with 70% ethanol to promote precipitation and centrifuged for 15 minutes at 3,000 g. Finally, the DNA pellet was resuspended in 200 μL TE buffer (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) containing 1 μg/mL of RNaseA and incubated for 1 hour at 37°C. The quality and concentration of the extracted DNA was evaluated on a 0.8% agarose gel stained with ethidium bromide [14]; the profile of the band obtained for the DNA samples was compared to a molecular weight standard with known sizes and concentrations (Figure (Figure2A2A).
Double-stranded DNA labeled with fluorophore was produced by Cy3-dCTP or Cy5-dCTP™ (Amersham Biosciences/GE Healthcare) incorporation during polymerization from 2 μg XAC total DNA (chromosomal + plasmid) that had been fragmented through the shearing technique (~14 times through a syringe with 8 cm and 18 gauge needles) [17]. The reaction was performed using 1 μL of the DNA polymerase Klenow Fragment™ (Gibco) at a high concentration (40 U/μL) and 500 μg of random nonamer primers [18]. Afterwards, the labeled DNA was purified with Multiscreen™ filter plates (Millipore), and the total amount of incorporated fluorophore was indirectly quantified through absorbance detection at 550 nm for Cy3 and 650 nm for Cy5. To ensure accuracy in the analysis, equivalent quantities of fluorescent DNA were used in the microarray hybridizations, which were performed for 16 hours at 42°C in hybridization buffer (Amersham Biosciences/GE Healthcare) with 50% formamide. According to protocols described previously by Koide and coauthors [18], after washing, the slides were dried by applying a jet of compressed nitrogen gas. Microarray data was aquired using a Generation III Scanner™ (Amersham Biosciences/GE Healthcare) in order to obtain images of each fluorescent channel, Cy3 and Cy5, for each of the microarray probes (Figure (Figure2A2A).
After the microarrays were scanned, the crude signal intensity of the images was determined using Array Vision 6.0™ software (Image Research/Molecular Dynamics). For each spot on the microarray, which represents a specific probe, the foreground and background intensity were measured.
Data preparation included subtraction of the local noise for each spot, and calculation of average fluorescece density and artifacts removal (MTM Dens). Array Vision software allowed the exclusion of bad pixels (pixels within a section that showed signal intensity either above or under four median absolute deviations (MADs) of the signal intensity of all of the pixels in the section).
The selection of clones from the XAC shotgun libraries allowed for the identification of 4,421 clones, which represented 3,084 individual CDSs of the XAC genome (Figure (Figure1A).1A). The remaining 1,405 CDSs, which would complete the genome, were not selected because they represented very small CDSs, smaller than 200 bp for example (Figure (Figure1B),1B), or because they were not physically found in the shotgun library [6].
Table Table11 and Figure Figure1C1C present the distribution and profile, respectively, of numbers of CDSs and selected clones, as well a schematic model of the CDS/insert overlap, which was used as a reference in the classification of the clones. It is worth noting that the ideal clones are classified as overlap type II, because the insert of these clones as a whole represents a part of the CDS of interest. Therefore, overlap of other genes or intergenic regions do not exist. However, this type of clone was the least representative in the XACarray and consisted of only 292 (11.1%) of the fixed CDSs (Table (Table2).2). For the other three types of selected clones (I, II and IV), an overlap region of other CDSs or intergenic regions with the insert was always present. Of those, the most representative overlap type was type I (see methods), totaling 1,184 fixed CDSs on the slide (44.9%) and presenting a insert/CDS of interest ratio of 0.76 (Table (Table22).
After the in silico selection, the clones were rearranged in 47, 96 well plates using a Q-Bot robot™ (Genetix, UK), and the inserts were amplified by PCR (see methods). Approximately 5% of the clones were shown to be not viable, and 10% presented an insert with a size different from that which was predicted (data not shown), suggesting the existence of probable errors in clone labeling, a common occurrence in clone library construction, even in commercial libraries [19]. Moreover, another 15% presented negative results with respect to their amplification. For these reasons, it was necessary to re-sequence one end of the insert of each of the previously selected clones in order to confirm the identity of all of the clones. The sequences were compared to the XAC genome, and the identity of the clones was corrected when necessary. Unfortunately, at the end of all the steps described above, approximately 30% of the initially selected clones (1,326) could not be used. Thus, at the end of these steps, expected identity of 2,653 clones were confirmed and this set was used. They were rearranged again, now in 28 96-well plates. Although the number of clones was severely reduced, the microarray contained a set of clones with a reliable identity. However, after the PCR was performed to increase the amount of the probe to be immobilized on the slides, the number of clones was reduced to 2,639, which represented 58.8% of the XAC genome (Table (Table2).2). To this set of 2,639 PCR products, 121 products derived from amplifications of genomic DNA sequences using specific oligonucleotides that were previously acquired for other experimental proposals were added; these products represented the sequences of genes of particular interest, including the genes vir, hrp, rpf and gum. Thus, the 2,760 PCR products were purified (probes), rearranged in 8 384-well plates, and used in the construction of microarrays as described above.
It is necessary to highlight that the molecular mass pattern obtained from the pUC19 plasmid not only facilitated the analysis of the amplified product, but also demonstrated that this approach was correct. The banding pattern quantification of some randomly selected PCR products corresponded to the visual quantifications that were performed using only the agarose gels (data not shown).
For an initial validation of the quality of the XACarray, hybridization with XAC genomic DNA labeled with distinct fluorophores was performed. Figure Figure2A2A shows the fluxogram of the XACarray protocol and the hybridization images obtained using this probe. Note that the spots on the slide are practically all yellow in color, which visually represents the hybridization of probes labeled with Cy3 and Cy5 in similar quantities. This result can be observed more rigorously from the monochromatic images obtained from laser scanning at 550 and 650 nm, respectively.
The analysis of these images revealed a noticeable signal above the background noise for 88.5% of the CDSs represented in the XACarray, a percentage that is equivalent to 2,365 individual CDSs of the XAC genome (52.7%). Table Table22 summarizes the physical features of the 2,670 probes deposited on the XACarray. The probes are represented in Figure Figure1C1C in relation to the type of overlap of the insert and the average size of these sequences. The classification of the CDSs regarding their annotation characteristics is shown in Table Table2.2. The CDS are classified into two major groups: CDSs previously annotated with a determined putative function, which represent 63.5% of the XACarray; and CDSs that compose the hypothetical group, in this case exclusive hypothetical and conserved hypothetical in other organisms, which represent the other 36.5% of the composition of the array.
Quantitatively, the XACarray can be considered to be representative of the XAC genome, not only for containing probes that represent more than 50% of all XAC genes, but also for presenting 83.4% of all of its annotation categories, with more than 50% of the functionally annotated genes (Figure (Figure2B).2B). This global vision of representation can be observed in detail in Figure Figure33 and additional file 1, which correlates the gene map of annotations to the CDSs fixed on the slide.
In this study, we developed a new and low-cost method to generate a DNA microarray. Previously, Astua-Monge et al. [20] used a DNA macroarray, implemented on nylon membranes and containing probes for 279 XAC genes associated with pathogenicity and virulence, to investigate genes differentially expressed in a synthetic medium (XVM2) that simulates in planta conditions. In contrast, our plataform is the first DNA microarray platform for XAC that representes more than 50% of all XAC genome CDSs.
The platform was constructed from shotgun libraries, previously generated for the genomic sequencing project. Although the clones of type II are ideal, because they are restricted to include just an internal fragment of the target gene, we have made an effort to select clones that would not overlap with other genes. In our array, there are at least 1,290 clones (~30% of the genome, not counting the ones for which specific primers were designed) that contain genome fragments that do not overlap any gene besides the target gene they were selected to represent. Also, many genes that have fragments in the same clone will correspond to operons and could, in a first crude analysis, be regarded as a single expression unit, with additional experiments clarifying which gene(s) are indeed relevant. Therefore, our platform can be used to generate lists of putative differentially expressed genes in XAC under different physiological conditions.
On the other hand, the presence of overlapping probes makes it necessary to subsequently validate individual candidate genes using methods with higher specificity and sensitivity, like qRT-PCR. Moreover, under most conditions, some more expensive methods, like designed-probe chips and RNA-Seq will always provide better specificity/sensitivity rates and better reproducibility and such methods should be preferred whenever researchers have access to them. Functional results using this platform were described in a recent work that use this tool to validated putative insertion/deletion regions between different and incomplete genomes sequences of other Xanthomonas species [21].
The authors declare that they have no competing interests.
LMM: Responsible for the amplification, purification and fixation of the material onto the slides, participated in the validation of the hybridization platform, preparation of the manuscript and elaboration of all figures; MLL: Participated in the amplification reactions and in the preparation of the manuscript; RFS: Responsible for assembling the software for in silico selection of the representative clones and participated in the preparation of the manuscript; PZ: Participated in the preparation of the manuscript; ACRS: Participated in the preparation of the proposal, the construction of the shotgun libraries and the assembly of the platform itself; AMS: Participated in the project design and the data analysis; JAF: Participated in the construction of the shotgun libraries, the data analysis and the preparation of the manuscript. All authors read and approved the final manuscript.
Additional informations about the CDSs highlighted in Figure Figure33.
We acknowledge the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for granting PhD scholarships to LMM, RFS, PZ and MLM and for funding the project (CAGE).
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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. Trail rides good and goes through and over everything. Smart Plan Smart Little Lena Peppy San Badger$5,000
. Maternal grandsire is Dunit Rawhide (1995 NRHA Limited Open Futurity Champion; 1995 NRHA Futurity/3rd Place$700
dozen more for sale. face book Diaman H Stables.$3,000
2014 APHA gelding. Cow horse blood line includes Ris Key Business Peppy San Badger and Bar B Paint$2,250
BUCKETTE. Her sire is Peppy San Jabalina by Peppys Brand who is by Peppy San Badger out of Little Miss$2,300
This is a sorrel filly that I got with a group of broodmares. She will walk right up to you$650
of Mr San Peppy. He has many world champions on his documents. His foals are performers SheNo Price Listed
of Mr San Peppy. He has many world champions on his papers. His foals are performers SheNo Price Listed


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Be intentional.
After I shared all my Truth Cards I had made myself at the end of last month I kept this one out. I set it within the frame in front of me so that I can see it. They end up right at eye level. When I chose this one I didn't realize how much I would need it this month. But oh did I ever.
I began struggling with ideas about mid-month working to know what direction I should take on a few things. I was struggling because I wanted to do what was best, I didn't want fear to hold me back, and I wanted to do what would thrill me, but everything had gotten mixed up. The problem was I began looking at some circumstances within my life and allowing them to have a heavier weight than they should have.
Oh, circumstances are definitely a player when we are living, right? There are some things we can't change, and there are things which change us. But I didn't want them to be heavier than everything else. Captain told me not to worry about those circumstances, but to do what I really felt I should do. He's so supportive that way. I also asked several good friends to pray for me, and to share advice if they had any. They were all great, and also encouraging.
Then I had to get quiet. I had to get pen and paper out and work through my thoughts. I had to listen to see what I was hearing from God. I had to close my eyes to it all and really consider. A few things I crossed paths with reminded me of where I wanted to be, and when all the information came together I realized the path I needed to be on was the one I originally stepped on. And then the peace flowed.
You see, I needed to be reminded of where I wanted to end up in order to know what I should do. And I know that not everyone will agree with my choices, but those that love me will still love me regardless. I'm not doing anything dangerous, or crazy, or stupid. I'm doing what I feel God is leading me to do right now. And I don't say that to shut up the critics, truly. This is where my path is, and so I am moving forward as I had originally planned, knowing the only thing which truly matters is what God thinks of me, and He's got my back.
As you move forward in your life I challenge you to be intentional. Look to see where you want to go, believe you were created for that, and take the steps you need to get there. Believe in yourself, and know that you are choosing your future based on what God has laid on your heart. Even if it doesn't make sense to everyone else.


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Nik filters?
Started Dec 7, 2012 | Discussions thread
Stakeouttoo • Contributing Member • Posts: 948
Re: Nik filters?
In reply to Howard Podgurski • Dec 8, 2012
I have the Nik Color Efex Pro 3.0 Complete that came with Paint Shop Pro X5 Ultimate when I did the Upgrade.. and at the 'right price' $41 ...a lot more Nik filters than I'll ever get to use.. seem to work very well..
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- post by: #TooRealForTV
- July 28, 2016
8 with ties to 'Polywood Crips' named in federal prostitution case "Facebook did it again." SMH
Two prostitutes at work in Arlington in 2007. In 2000, Fort Worth police posted signs in areas where neighbors had complained about prostitution. Two prostitutes at work in Arlington in 2007. In 2000, Fort Worth police posted signs in areas where neighbors had complained about prostitution. BY MARK DAVID SMITH [email protected] Six people — most […]Read More
- post by: #TooRealForTV
- July 26, 2016
How did this guy become a pimp? SMH Woman forced into prostitution by man she met on Facebook: Cops
BY DANIEL MCKENZIE, TORONTO SUN FIRST POSTED: MONDAY, JULY 25, 2016 12:37 PM EDT | UPDATED: MONDAY, JULY 25, 2016 06:51 PM EDT Ankush Avishek Monebhurrun, 21, is charged in a human trafficking investigation in Toronto. TORONTO – A woman who met a man on Facebook was forced into the sex trade after moving to Toronto, police […]Read More
- post by: #TooRealForTV
- July 25, 2016
Alleged shooter rejects plea offer in prostitute slaying. If convicted he could face life.
Guastella also said she informed the defendant he might not get another offer which did not involve a potential lifetime sentence. October could face 25 years to life in prison if convicted at trial of second-degree murder. Justice Stephen J. Rooney ordered pretrial hearings for Aug. 11. The slaying unfolded around 3:20 a.m. Sources with […]Read More
- post by: #TooRealForTV
- July 20, 2016
Former police officer quits force to become a prostitute.
.Former cop swaps thin blue line for life as escort Meet the woman who has moved from the thin blue line to the red light world. Single mother Heidi was a senior constable. But these days, she's earning a living as a high-class escort. A Melbourne single mother has traded her role as a senior […]Read More
- post by: #TooRealForTV
- July 19, 2016
Former teen prostitute gets married and a 2nd chance at life plus green card.
BY JOHN MARZULLI NEW YORK DAILY NEWS Friday, July 15, 2016, 9:33 PM Human trafficking survivor Carmen (r.) married her fiancé, Luis (r.), in a ceremony officiated by Judge Pamela Chen (c.), who prosecuted Carmen's traffickers. Human trafficking survivor Carmen (r.) married her fiancé, Luis (r.), in a ceremony officiated by Judge Pamela Chen (c.), […]Read More
- post by: #TooRealForTV
- July 19, 2016
How a Boyfriend in debt turned out girlfriend to be a prostitute.
By Jane O'Gorman / Published 17th July 2016 STOCK IMAGE GETTY REVEALED: He says I should be prepared to help him out A business venture with an old mate went badly wrong and he is now over £200,0000 in the red. He has sold his car, his record collection and all of his gadgets. Now […]Read More
- post by: #TooRealForTV
- July 19, 2016
Amateur Prostitution in America – Extraordinary Documentary 2016
Read More
- post by: #TooRealForTV
- July 15, 2016
Seattle PD undercover sex sting nets more than 200 arrests. (Video) This is entrapment. SMH
More than 200 Pacific Northwest medical doctors, PhDs, CEOs, construction workers, students, tech workers, a long-time news industry employee and more have been arrested over the past week in a prostitution sting set-up by the Seattle Police Department. For the first time ever, Vice Detectives opened their own "brothel" to make the busts, leading […]Read More
- post by: #TooRealForTV
- June 24, 2016
Elementary teacher arrested in prostitution sting
HYATTSVILLE, Md. (AP) — Authorities in Maryland say a Prince George's County elementary school teacher has been arrested in a prostitution sting operation. Officials say 29-year-old Martin Rye Brown of Silver Spring was arrested Tuesday on charges of prostitution and human trafficking. Brown is accused of soliciting a prostitute and renting a room that was […]Read More
- post by: #TooRealForTV
- June 08, 2016
'Game of Thrones' saved this actress from a life of prostitution
A wannabe actress who earned her living as a prostitute before finding fame on "Game of Thrones†says her life was saved by the hit TV show. Josephine Gillan made her acting debut as novice prostitute Marei being shown how to fake an orgasm in Season 2 of the fantasy drama. She says she used her […]Read More
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Results in Patio Traditional Sconces Light Holders
1-4 of 4
Pair Of Wall Mount Black Metal Candle Holders Decorative Candlesticks Sconces HoPair Of Wall Mount Black Metal Candle Holders Decorative Candlesticks Sconces Home Decor In good condition, wear and tear that comes with old age, but nothing serious, please see pictures for more details. Dimensions : 38cm tall, 13cm deep and the candle plates measure 10cm in diameter Collection from Bognor Regis or I can ship for £ 3.99.£13.99£3.99 postage
Candle Sconce - Blacksmith Hand forged in EnglandThese our Hand forged Candle Sconces. They have a 75mm diameter drip tray for the wax and the candle sits 70mm away from the wall. They have a small spike in the bottom of the drip tray to locate the candle and to prevent it from falling.£14.95Click & CollectFree postage
Antique Gold Wall Mounted Candle Sconce by DiborCrafted from lightweight metal with a distressed aged effect antique golden finish this wall mounted candle holder features a traditional shaped design perfect for adding elegant classical style to your home.£29.99Click & CollectFree postage3 watching
Blacksmith Style Wall Mounted Candle Holder Wall SconceDelightfully classical in design this candle holder has been traditionally hand forged from wrought iron. The wall mounted candle rest has been finished in a sleek midnight black and is beautifully simple in detail.£45.99Free postage


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MySQL is an awesome, free Mac program and has been created by Mysql.
More about MySQL
About the download, MySQL is a not that heavy software that does not require as much storage than the average program. It's a program very heavily used in countries such as India, United States, and Pakistan.Since we added this software to our catalog in 2012, it has managed to reach 39,921 downloads, and last week it had 18 downloads.It's available for users with the operating system Mac OS X and former versions, and you can get it in English. The software version is 5.5.30 and its last update was on 11/03/2013.


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AlienVault® Unified Security Management™ (USM™) is an innovative approach to security monitoring, delivered in a unified platform. The USM platform includes five essential security capabilities that provide resource-constrained organizations with everything they need for effective threat detection, incident response, and compliance management, in a single pane of glass.Advertisement
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Contact Form WordPress Plugin
Add Contact Form to your WordPress website.
Contao Open Source CMS
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Crabgrass is social networking, group collaboration, and network organizing Web application. It consists of a solid suite of group collaboration tools such as private wikis, task lists, a file repository, and decision making tools.
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Minimize application downtime by resolving application issues 65% faster. Monitor applications across on-premises, hybrid, and public cloud infrastructures from a single console. Set up intelligent application performance alerts to find and fix issues before they impact business services and end-users. Monitor 200+ applications from a single dashboard—no more wasting time between multiple tools and custom scripts.Advertisement
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LimeSurvey - the Online Survey Tool
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ThousandEyes extends visibility across corporate networks as well as the public Internet, helping to solve issues from the branch through MPLS links and SIP trunks to service provider networks. Simulate pre-deployment capacity, monitor detailed performance metrics and see how QoS settings impact call quality.Advertisement
MisterEstate is a 3rd party component for the popular Joomla CMS and enables small real estate and facility management companies to present their properties in a pretty easy way to the world wide web.
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A great blend of fresh ground beef, with a great combination of toppings with delicious fresh cut French fries! My only regret is you cannot get it cooked as rare as I like!
1 - 10 of 102 reviews
A great blend of fresh ground beef, with a great combination of toppings with delicious fresh cut French fries! My only regret is you cannot get it cooked as rare as I like!
This was no different from most "Five Guys". If you like small burgers and greasy chips, you'll love it. My favorite thing is the peanuts!
Burgers and Fries to die for! They are soo friendly! Great shakes too! Everything is so fresh and service is quick!
Everything is so delicious. Especially the peanuts. Love it. Hehe. Friendly staff, delicious burger.
We stopped in for lunch during a shopping trip to North Port. We both ordered burgers of various descriptions and french fries. Both of us enjoyed our lunch although I was disappointed that the restaurant did not offer cheddar cheese for the burger. Considering the...More
We like the burgers and fries at 5 Guys! Always made to order and so moist and yummy. The fries are outstanding! It is family friendly and also is a great place to go with couples.
Please do go here asking for a low carb option, or complain the fries are " greasy". This is a good ol' fashioned hamburger joint. Real meat, fried in their own fat. Real potato fries, cooked in oil. It's a hot, delicious mess. We love...More
It's four years since I was in a Five Guys and I was looking forward to an orgy of gourmandery. Either my memory has failed me or the Five Guys cookery has slipped. First: This is not health food. If you want something that your...More
Fries tasted like old grease or were refried. Not sure. When I went to eat one on the way home, I couldn't believe it!! Then when i got home, they were all bad. I even took a pic to show the manager if I needed...More
Friendly people! Awesome, hot fresh food every time I've been here. Kind if pricey, but worth it. You won't leave hungry.


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HARRISBURG: More arrests made in Thursday night shooting incident
Two more arrests have been made in the insane debacle that went down late Thursday night (June 22) into early Friday morning (June 23) on West Church Street in Harrisburg.
Two more arrests have been made in the insane debacle that went down late Thursday night (June 22) into early Friday morning (June 23) on West Church Street in Harrisburg.
Arrests have been made in the incident last night that has an edgy Harrisburg even edgier.
Details are scant, but another shooting has been reported in town, this one late last night/very early this morning (Friday, June 23.)
Gangsta-wannabes, be aware: You might want to take your unintelligible talk someplace where people might actually be intimidated by it.
Palestine and Crawford County officials made a stop last night that was somewhat stunningly multi-county…and brought in a girl who was part of a huge controversy in Jasper County a few months back.
HARRISBURG – Just weeks after the community of Harrisburg came together to try and resolve some of the issues many believe are driving some of the city's young people to a life of criminality, a man reportedly uninvolved in the criminal activity is dead, literally a "bystander" victim of the local gangsta-wannabe group terrorizing the town.
HARRISBURG – With all the other happenings in Harrisburg, one might not think that an Order for Demolition of Structure case is much of a big deal…and it isn't, by comparison, but nevertheless, it's going on.
SALINE CO.— A Harrisburg crim claimed he was merely taking a leak near someone's home when he accidentally ended up trying to steal a satellite dish.
SALINE CO.— A former Eldorado man has been charged with sexually assaulting a young child.
SALINE CO.— A Harrisburg woman is facing a felony drug charge after police say she was slumped over in an alleyway in her vehicle.


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The American Association of University Women is open to all graduates who hold a two year associate degree (or equivalent), baccalaureate or higher degree from an accredited college or university.
AAUW promotes equity for all women and girls, lifelong education, and positive societal change. In principle and in practice, AAUW values and seeks a diverse membership. There shall be no barriers to full participation in this organization on the basis of gender, race, creed, age, sexual orientation, national origin, disability, or class.
Membership Application Form New member application form 2017-18
AAUW Brevard Activities Interest Form AAUW Brevard Activities Interest Form


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Tips for Keeping Your Cool with Your Office Wardrobe
Raquel Greer shared a few do's and don'ts you might want to follow to keep it professional.
Raquel Greer shared a few do's and don'ts you might want to follow to keep it professional.
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Coroner's officials ruled Fisher died from sleep apnea and a combination of other factors.
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This is already old news, but I did not have the time to post it before. Overall, the numbers show economic conditions worsening due to lack of domestic demand. The financing needs gap has been closed. The trade balance continues to improve because of the decease in imports. The most relvant development since the previous quarter is the slowdown in exports.
"The twenty greatest albums of 1967"
3 hours ago


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Moments prior to the Kick-off for the TN10 Filmmaking Challenge, a panel of local filmmakers talk about last week's 54 Film Fest and we reveal the required elements for the TN10.
The 10 filmmakers selected for the Knoxville Film Festival TN10 Best of the Best Filmmaking Challenge are revealed.
Keith McDaniel and guest, Jacob Boyd, discuss 2017 film festivals in East Tennessee.
Knoxville Film Festival Director Keith McDaniel discusses plans for the upcoming 2017 KFF with guest Jeff Snell.


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Shiny White Shag $350
Originally $1450.00, this shiny, white, shag runner was hand-knotted using bamboo silk. It is a one of a kind rug with a high pile and a two inch border around the edges. You can choose either a cream trim or a dark black trim.
Size: 2' 09" x 7' 00"
Quality and hand-knotted area rug at an unbeatable deal.
$ 350.00
Item added to basket!


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Marking the 44th anniversary of JFK's assassination, a crowd gathered today in Dealey Plaza in Dallas, where witness Ernest Brandt shared his account. Meanwhile, an assassination plot to kill JFK in Chicago was recently revealed.
Chad Calek discussed his new documentary which chronicles the paranormal investigation that led to the filming of a full-body apparition.More »


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Japanese metal band Dir En Grey have released a video for Different Sense, which comes off the upcoming Dum Spiro Spero album (review here). Check it out after the jump to catch a glimpse at what their live show is like. Also, make sure to check out Kyo's Top 10 list and our interview with guitarists Kaoru and Die.
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this week in horror
This Week in Horror - June 12, 2017 - Starship Troopers, Godzi...
An animated Starship Troopers movie is coming to theaters, Godzilla vs. King Kong has its director, and more details emerge about Jeepers Creepers 3. It's This Week in Horror with Whitney Moore!Posted by Bloody-Disgusting on Monday, June 12, 2017
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Adaptation of Stephen King's 'IT' Receives Delicious "R"-rating!


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Deadtime Stories: Volume 1 (US - DVD R1)
Millennium Entertainment has revealed details on this new July 12th release
Starring: N/A (Various)
Released: 12th July 2011
Further Details:
Millennium Entertainment has announced Deadtime Stories: Volume 1 for July 12th. Retail will be around $24.99. The first volume of this two-volume set from horror master Romero consists of an anthology of three 30-minute spine-tingling horror films from writer Jeff Monahan (Lone Star), who also directs alongside Michael Fischa and Matt Walsh. Artwork is attached:
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We'd never pan-fried halibut with an almond crust before. This gluten-free Bon Appetit recipe taught us a unique twist on breading, and we'll be using this technique again. Also, the curry-carrot juice base makes a memorably delicious foundation for the fish.
Make the curry base Heat 2 T olive oil in a medium pot over medium-low heat. Add 1 cup chopped carrots, 1 cup chopped celery, 1 cup chopped onion, 1/4 cup peeled, chopped ginger, and 4 large chopped garlic cloves. Cook until the vegetables are soft – 10 to 15 minutes.
Turn the heat up to medium-high and add 3 T Thai yellow curry paste. Cook, stirring constantly, until the paste starts to caramelize – 2 to 3 minutes. Then add 4 cups carrot juice and turn the heat up to high. Simmer until the juice is reduced by half – 15 to 20 minutes.
Strain the curry thru a sieve into a large bowl and discard the solids. Return the broth to the same pot and add 1 cup canned, unsweetened coconut milk and 3 cups of chopped, mixed vegetables (we used bell peppers and yellow squash). Cook over medium-high heat until the vegetables are tender – 8 to 10 minutes – stirring occasionally. Season with salt and pepper.
Halibut Dipping Station Creation Preheat the oven to 350. In a food processor, grind 1/2 cup of slivered almonds until they're 1/8″ pieces (don't make almond butter!) Put them in a wide, shallow bowl. Season both sides of 4 4-oz halibut fillets with salt. Whisk a large egg white in another wide, shallow bowl until it's just frothy.
Dip a filet in egg white, then in almonds (pressing so they almonds stick), and put the fish on a plate, nut-side up.
Heat 2 T olive oil in an ovenproof skillet over medium-high heat. Place the fish, nut side down, in the pan. Cook until the nuts are golden – 3 to 4 minutes. Turn the fillets over and transfer the skillet to the oven. Bake until the fish is just opaque in the center, about 4 to 5 minutes.
Divide the curry base among wide bowl. Top with the halibut, crust side up. Garnish with basil.
- 2 T olive oil
- 1 cup chopped carrots
- 1 cup chopped celery
- 1 cup chopped onion
- 1/4 cup chopped peeled ginger
- 4 large chopped garlic cloves
- 3 T Thai yellow curry paste
- 4 cups carrot juice
- 1 cup canned, unsweetened coconut milk
- 3 cups 1″ pieces mixed vegetables (we used red bell peppers and squash)
- 1/4 cup slivered almonds
- 4 4-oz skinless Pacific halibut fillets
- 1 large egg white
- 2 T olive oil
- Fresh basil leaves
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Choosing An Anti Aging Skin Care Product That Really Works
Choosing An Anti Aging Skin Care Product That Really Works
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2010 Honda Insight for Sale in Tulsa, OK
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|by Chryssi
"The Honda Insight is a very dependable car and I highly recommend it to anyone!!!"
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Stop by Red Hot and Blue in Annapolis and register to win the BEST view of the Annapolis Fireworks this year - from 30 feet above the City Dock in the WRNR Skybox!
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Unformatted text preview: BE/APh161: Physical Biology of the Cell Winter 2010 When: TTh, 8:30-10:25 AM. Because I will be on travel several times through- out the term, the hours are set so that we don't have to do makeup lectures. Who: You and me (Rob Phillips, x 3374, [email protected] , 159 Broad). The TAs for the course are Stephanie Johnson (x3333, Broad 132, [email protected]), Linda Song ( [email protected]) and Chao Liu ([email protected]). I am always happy to see you, but with cer- tainty, the best way to contact me is by email. After that, the best approach is to schedule a time to see me through my assistant, Katie Miller (x6337, [email protected] , 138 Broad). Where: 24 BBB What: See below! How: Lecture twice a week and weekly homework. No exams. Your grades will be based upon your homework grades. I will NOT accept ANY late homeworks (late means anytime after class starts the day the homework is due) unless you have a mindblowingly good excuse - this means a note from someone like a doctor or a Dean. (Please do not even ask me for an exten- sion - the answer is NO). As for collaboration, you may discuss the homework with others, but your explanations and derivations must be your own and your logic should be carefully explained and the significance of your results should also be explained. If you hand us a sloppy (either sloppy thinking or 1 writing) homework the grader will likely be unable to penetrate your logic and you will lose points. Reading: The course webpage will have a repository of required reading from the original literature. I am particularly persuaded that wide ranging reading will maximize the value of this course and will be providing a wide variety of opportunities for you to explore different topics. There are two main texts for the course: A Feeling for the Numbers in Biology by Ron Milo and Rob Phillips. This book is in the early stages of being written and is meant to complement the recently developed BioNumbers website (http://bionumbers.hms.harvard.edu/). Physical Biology of the Cell by Rob Phillips, Jane Kondev and Julie Theriot. There will be required readings from this text as well as many homeworks that come from the text itself. 1 Course Overview and Philosophy It is a wonderful time to be thinking about the workings of the living world. Historic advances in molecular biology, structural biology and the use of phys- ical techniques such as optical traps have provided an unprecedented window on the mechanics of the cell. The aim of this course is to study the cell and its components using whatever tools we need in order to make quantitative and predictive statements about cellular life. The main intellectual thread of the course will be the idea that the type of quantitative data which is becoming routine in biology calls for a corresponding quantitative modelling framework. The plan of the course is to elucidate general principles with exciting case studies. In 2009, having just completed my book "Physicalexciting case studies....
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This document was uploaded on 01/03/2012.
- Fall '09
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2010 Jeep Compass for Sale in Rochester, MN
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|by Moises
"I would get it again if they make a more powerfull engin for it."
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As expected, StanCorp earnings well short of expectations
As expected, StanCorp Financial Group on Monday released one of the most disappointing earnings reports in recent Oregon history.
The Portland-based insurance and financial services company (NYSE: SFG) warned investors last week that it would likely come up well short of analyst expectations for the second quarter because of high claims cost in its long-term disability insurance business.
It did exactly that.
The company on Monday reported second-quarter earnings of 51 cents per share, excluding after-tax net capital losses. The results fell 35 cents per share below what analysts had expected before last week's warning.
"Claims experience in our group long term disability business continues to reflect the headwinds of a weak economy including high unemployment and low interest rates," chairman and CEO Greg Ness said in a news release. "We are committed to obtaining and retaining profitable business and are actively taking pricing actions on both new and renewal business to address the unfavorable claims experience and the continued low interest rate environment. Despite the economic pressures on our group long term disability business, we continue to maintain a strong balance sheet and capital position."
Despite coming up short of analyst expectations, the company remains profitable. It earned $20 million on $725.3 million in second-quarter revenue, compared with $17.8 million on $681.4 million in revenue in the second quarter last year.
The company released earnings after the market closed. Shares were flat in after-hours trading at $31.31. Shares have fallen more than 15 percent since last week's warning. They have a 52-week range between $24.96 and $41.99.
StanCorp is Oregon's third-largest public company and downtown Portland's largest employer. It had $2.8 billion in 2011 revenue. Roughly 80 percent of its nearly 3,000 employees are in Portland, according to the company's most recent annual statement.
Matthew Kish covers footwear, apparel, banking, finance and general assignment news.