text:
The First 48 Season 16 Episode 17 (S16E17) - Watch Online
The First 48 is an American documentary television series on A&E. Filmed in various cities in the United States, the series offers an insider's look at the real-life world of homicide investigators. While the series often follows the investigations to their end, it usually focuses on their first 48 hours, hence the title. Each episode picks one or more homicides in different cities, covering each alternately, showing how detectives use forensic evidence, witness interviews and other advanced detective skills to identify suspects. While most cases are solved within the first 48 hours, some go on days, weeks, months, or even years after the first 48. The shortest investigation was led by Det. Brenda Wescott, who arrested a suspect, just 6 hours into the investigation. Dion Graham is featured as the narrator, and is the only member of the cast to be in every one of the episodes.The series was nominated for a Distinguished Documentary Achievement Award in the Continuing Series category by the International Documentary Association, eventually losing out to American Experience. By season 6, The First 48 had become the highest rated non-fiction justice series on American television, and had gained considerable critical acclaim along with controversy. The season 8 premiere, "Gone", which aired on January 1, 2009, garnered a domestic audience of 2.3 million viewers making it the series' most watched episode, at the time.
The First 48 Season 16 Episode 17 (S16E17) Links


text:
Click for "Microbes After Hours" videos
Robert Koch struggles with the disadvantages of using liquid media for certain experiments. He seeks out alternatives, and first uses an aseptically cut slice of a potato as a solid culture medium. He also turns to gelatin, which is added to culture media; the resulting mixture is poured onto flat glass plates and allowed to gel. The plate technique is used to isolate pure cultures of bacteria from colonies growing on the surface of the plate.
Gesundheitsampte 1: 1-48. In Milestones in Microbiology: 1556 to 1940, translated and edited by Thomas D. Brock, ASM Press. 1998, p101 [pdf]

subset: commoncrawl


text:
Contact Information
Women & Children's Hospital of Buffalo
219 Bryant Street
Buffalo, NY 14222
- (716) 878-7681
- (716) 878-7440 Fax
- Map & Directions
Child Life Department
The role of the Child Life Department is to provide support, facilitate coping, minimize emotional trauma, and encourage normal growth for infants, children, and adolescents.
Importance of Play
Play provides a non-threatening experience for children to express feelings and gain control of situations. Child Life encourages patients to play while in the hospital to provide a safe and familiar environment despite stressful surroundings.
- Provide age-appropriate play, creative arts, social interactions and other activities, encouraging mastery, coping, and expression of feelings.
- Bridge the gap between home and the hospital by providing familiar activities.
- Ease children's fears and anxiety that is often associated with being in the hospital.
- Prepare and support patients and families during their medical experiences in terms that they can understand.
- Serve as a resource for patients, families, and other staff regarding child development and the needs of children undergoing a medical experience.
- To encourage children and adolescents to maintain a sense of independence, self-esteem, and control.
- To provide essential life experiences/reestablish familiar routines which are developmentally important yet may be disrupted while being hospitalized.
Services Offered:
- Bedside Activities
- Bereavement Support
- Developmental and Age Appropriate Activities
- Dog Therapy
- Family Support
- Holidays/Birthday Celebrations
- Individualized and Age Appropriate Pre-Procedural Teaching
- Medical Play/Education
- Pre-Hospitalization Tour
- Procedural Accompaniment/Distraction
- Special Guests and Entertainment
- Pre-Hospitalization Tour
- Special Guests and Entertainment
- Supervision of Child Life Volunteers and Interns


text:
'Bill Savino's advice to the Rigases: "That team's not worth $200 hundred million. There's nothing there for you. Let the NHL take it over, sell it, and you worry about your other problems because you've got plenty."'
NHL, Adelphia to discuss future of Sabres
June 13, 2002 Submitted by
Via: WGRZ-TV


text:
'The Sabres were down to employing the No. 9 defenceman in their organizational depth chart, Jeff Jillson, for last night's third game against Carolina. They already had No. 7, ex-Bulldog Rory Fitzpatrick, playing as a No. 4 for mainstay Dmitri Kalinin, whose fractured ankle will keep him out a while yet, and used No. 8, Doug Janik in place of Teppo Numminen in Game 2, before replacing him with Jillson.'
Big 'D' rears its ugly head
May 25, 2006 Submitted by
Via: Hamilton Spectator


text:
Energy-bustin' fun is just a hop, skip, and giggle away! Kids have a blast—and get a great workout—keeping up with this musical skipping game. Height adjustable, with three speeds, to challenge different ages and skill levels. Plays six songs. Let's jump together! Requires 3 C batteries (sold separately). For ages 3 and up. Colors may vary.For more information, click here.
Sign in to your Address Book.
To check if any specific restrictions for this item apply to any United States shipping address, please enter the information below.Sorry, this service is not available to Canadian addresses.
This is being shipped to a P.O. Box
This product has no shipping restrictions.
© 2017 One Step Ahead


text:
'Tailgaters reveled in the parking lots on Perry Street. Flocks of fans exchanged high-fives with motorists backed up in the post-game traffic. The five-beat "Let's Go Buffalo" war cry echoed incessantly, mixed together with random, joyous blasts from car horns. As I sit write this article, tucked in an apartment in the heart of Allentown, the horns still blare throughout the city.'
Can you believe it, Sabre fans?
May 11, 2006 Submitted by
Via: Dunkirk Observer


text:
Discussion in 'The Trash Can' started by PashaFan, Nov 27, 2013.
If you can find a judge to bribe we can all make a donation !.
Ha! That's adds new content to the expression fan support.
They need Volosozhar and Morozov to get back together so that Trankov gets jealous and has a diva moment and refuses to train.
Quite a soap opera plot turn!
I have not seen either V/T´s or S/S´s this season´s programs live. One of the judges in NHK Trophy I spoke wit said that V/T live are simply breathtaking. Looking forward to see both of them in a few days in Japan.
Actually, Nikolai Morozov should seduce Tatiana ruining that partnership and then in a jealous rage, Elena and Nikita will train their butts off with Shpilband until Sochi!
I think we got the answer today. Skate clean and with higher base value than V/T. The 3t 3t seq is one key.
And have V/T make two major jump errors.
They just need to keep it up. They don't need a 3axel, they need to push the positions and finishing touches now to the limit. Go after 10s in every component, especially transitions. They are one of the only teams capable in the world of deserving a 10 in transitions too, but they'd need to re-work some things. They are right there, it's nice to see a real battle heating up. The LP in particular needs to be more in synch with the music and pushed a bit more emotionally, it's too literal and not enough of the emotion and tension in the music yet IMO. A huge step up in both programs IMO and they definitely won't settle for silver. Personally if them winning the OGM means we get another 4 years of Volosozhar/Trankov, then well.. I'm all for it I do not want to lose both S/S and V/T.
However I do think they completely deserve it on their own merit, they've done every program possible and then some and always seem to fight like tigers when people count them out. I'm going to miss them when they're gone, they've been nothing but a breath of fresh air since that voidy SP they debuted back in 05-06.
So happy for Aliona & Robin but kinda sad that it was still so close. V/T made to major errors & still were in touching distance.
But let's hope Aliona & Robin can get better & better from here. I still believe they can win in Sochi........................maybe.
Thrilled for S/S today. That's the team I know and love.
Funny I feel the same way Karpenko. That maybe if V/T don't win they'll stick around. It's selfish but I don't wanna lose them both either.
To your last sentence I agree 100%. They have been one of the greatest, they have done it all,tried it all, never shyed away from anything. They have done the outrageous, the complex, the dramatic, the beautiful, the heart-wrenching and I've loved every minute of it! They are one of a kind and I will miss them tremendously when they retire, the will leave a big hole no doubt, but this sport, and pairs in general is better and stronger for having had them around. I just feel so lucky we got to have them for so long, yet it's gone by, like everything, much too fast.
They need to skate like today ! They have more chemistry to me than V/T but I'm an ice dance fan so what do I know
I enjoyed S/S more .V/T only comes alive in the last twenty seconds at the music change.
In fairness, I think it might actually be physically impossible to not have chemistry with Robin Szolkowy - few partners seem as generous yet still a "leading man" in the partnership.
Today shows all the silliness of the talk about the judging all along. V&T were winning every event since they deserved to, and S&S won today just as they deserved to with their wonderful competiion. Whoever skates best will win in Sochi once again.
I do wonder if V&T dont win in Sochi (which I still think they will) if they would stay another 4 years. I would hate the pairs field to lose all of S&S, V&T, and P&T in one fell swoop. It would become almost unwatchable.
I think that is my favourite S/S program ever. I confess I've never liked their other programs very much (except for the Frida? program), but this one makes clear their high quality. I have often thought they won titles because of a lack of strong competition (until V/T came along) but with this program, and particularly their performance today, I can see their strength. And you can never say they didn't give it their all... a clean throw at the very end of their programs is what I call brave and gutsy and they are definitely that.
That was beautiful! sooooooooo happy for them! hope is still alive.
S&S are right up there with G&G as my all-time favourite pairs team and I want that OGM for them so badly. I love V&T too, and think they are equally as deserving, but since there can likely only be one winning pair, my heart is pulling for Aliona and Robin.
Even though their programs transition-wise this year aren't as difficult as in past years, they still lead the field there, imo, and should get credit for that. I hope this victory and beautiful skate gives them a boost. This is going to be an epic battle for the Olympic title and I feel privileged to watch.
I kept looking for a thread to gush about how happy I was for them at this comp!! Not just because they won, but because they were clean! Those 3ATh were killing me. And that long set up for it really took away from the short for me. I think their short this weekend was magnificent and breathtaking! And they were so thrilled afterwards! This was the first time this season I've liked them over V/T. I think they're really starting to click with these programs. (I will complain that Robin's shirt was less see-through and his pants were less tight, booo )
Side note: Mozer did an interview (translated somewhere in GSD) where she said V/T will stay in as long as their bodies will let them. That they feel they're just hitting their prime and aren't ready to quit.
I would love to believe that, but the reality is V/T made huge errors that couldn't be swept under the rug of "gray area". I think if they skate close V/T would win. With that said P/B got Bronze in dance, so perhaps someone is cracking with whip on all the Russianflation or they're lulling us into a false sense of security.
Like I said V/T deserved all their wins, but it doesn't mean there is a huge judging bias this Olympic season. V/T have been pretty consistent, so you can't expect them to make huge errors again, unless they don't do as well under pressure.
I almost fell out of my bed this morning, I was so excited !!!
I don't know how reflective this GPF was of Olympic possibilities, but I'm happy they're back in the game. I'm sad about the throw triple axel. I really wanted them to do it. I would have been a great thing for them to be remembered by, but I'm happy to see their super huge gravity defying throws back and polished. You can tell how much effort they were really putting into it, because all their other elements look so much better now that they've stopped.
I am so happy for them. They skated so perfectly clean. I hope they can repeat this in Sochi!
They looked so thrilled during the medal ceremony.
So pleasantly surprised when I woke up this morning and heard that S/S won!
lmarie, I think you will love their LP! Here it is:
And here is their SP:
I am still on cloud nine.
What they can do or hope:
1) call Tonya Harding for physical advices
2) Johnny Weir and Maxim officially announce their relationship at the Olympic so poutine will apply his new law
3) Tatiana join the pussy riot and to poutine, so she end up in jail
On a more serious note, they have to be perfect and hope V/T make at lest one major mistake. It's sad but if the russians are clean in the short and free, combined with the politicks (cheating ) and home advantage, it would be almost impossible for them to win...Hope I'm wrong !
Peter Carruthers in commenting today kept on mentioning that V&T messed up bc S&S really got to V. The Olympics is largely a mental thing so it might have done them good to see how they skate when S&S skate well. It obviously psyched V up, so she knows now she has to work on how she deals w that mental issue should it happen at the Olympics.
Yes, one key for S/S in Sochi would be to stay calm and in control, and instead of suffering the pressure, put pressure on V/T.
It felt so good to see them skate clean again after all of their struggles last season and earlier this year. I hope they can repeat these performances again in Sochi!
After the Grand Prix Final
anything is possible.


text:
|SVT Performance (2002-2004) The place to chat about any 2.0L SVT DOHC Zetec engine performance, tuning and exhaust related upgrades.
|Topic Review (Newest First)
|If no one was ever wrong, there'd be very little to talk about and very few users posting.
Takes a big man to admit his mistakes.
And, I for one still trust your knowledge.
Well, I figured I could be wrong on that part of it, although I believe I am still right on the "gapping " part. Totally pulled the hot/cold plug thing out of my butt. (just so you guys know, I don't do that much, so I'm usually trustworthy I swear!)
I would expand more on it, but like WD40, I can't do any better than the link he provided.
Hot and Cold refers to the temperature that the tip of the plug can reach.
A hot plug will not allow any heat to escape to the outer metal portion and will run hotter,
while a cold plug will be just the reverse.
A hot plug will have a lot longer insulator nose than a cold plug.
IOW, a cold plug will have more contact between the insulator and the metal surrounding it,
in order to transfer heat and keep it running cooler.
There's got to be a better explanation than what I can do....searching...
Here ya go...>>> Clicky
|i got the SCO grounding kit delivered yesterday - great quality.
|I did have it done, but you know, I never did confirm it. The car felt so much more responsive and didn't bog as much after I got it from the dealership that I never checked to make sure. I'm sure I'll eventually get a tune for the car since it's legal in STS.
|I didn't see this mentioned anywhere, but have you done the YRFO reflash for your SVT? I have an 03 and mine used to stall like that, especially under braking at low speed. Sorry, I didn't have time to finish my term paper on ignition systems, but I swear I will have it in by tomorrow.
^^It's just the "Geek Gene" running strong and true. I love being a geek and I am darn proud of it!
If you really want some seriously geeky conversations that usually spiral far beyond my understanding, try some Nasioc.com or sccaforums.com threads. When you start to use advanced math to explain your way though a slalom, and other people come back with even more complex math to refute it, you know you are in a whole other world....
Ah, I gotcha now. The purpose of the gap is to allow a larger amount of current to be released, whereas if a shorter gap were used, it's possible that not all of the current present would flow across the gap (it might travel back into the system more or less). Everything works like a resistor in the system, and you want the air gap between the node on the spark plug and the ground point on the other end of the plug (grounded by the block of course) to be the largest resistor allowing for maximum energy dissipation at that point, the gap.
And I really don't think there's necessarily anything wrong with the term potential energy since there's still a greater amount of energy at the higher end of a potential gradient, but I think the term is just confusing when relating it to classical physics and mechanics. Sorry, I've been lectured and corrected on that stuff so much in my degree up to this point...
Term paper... ha! You should see some of the posts I've posted on other sites before. I've done posts from 4 to 6 pages long (I actually reached the character limit for the post and had to do two!). I didn't get too many responses either... lazy users. I think you were talking about term paper as in lecturing over technical stuff. I get off on talking about these things... call me weird.
I don't believe that it's a "longer" spark per se for widening the gap. My understanding is this;
To have a spark occur you must have 2 poles with potential energy (yes, I know that it's probably the wrong term there, but i think y'all get it). Once the potential energy reaches a certain point then it will create the spark because the energy is at a high enough level to overcome the resistance to it taveling through the air. So basically it powers up until it overcomes the resistance, and then once it makes the spark the potential energy is spent.
By lengthening the distance between the 2 points the resistance is increased. Therefore more energy must be created for the spark to occur, and thus when it does spark there is more energy in it, which combusts the fueal/air mix more completely. More energy to drive the reaction.
The whole reason that people buy "colder" plugs is that the materials used in those plugs allow more energy to be released in the actual spark, without requiring more energy from the system. A "hot" standard plug will retain a lot of that electrical energy in the material of the plug itself, which means less is transferred in the spark, whereas the cold plug has more efficient materials that will allow more of that energy to be discharged in the spark. If you know about speaker systems, that resistance in the plug (and the air?) is termed Ohms. IE, that is a 12 Ohm resistor.
This is much the same way the uprated wires work, in that they have less resistance and therefore allow more of the current to flow through unabsorbed (or escape).
Thus concludes my term paper on ignition systems. If any of that is wrong, please remember that I just pulled that right out of my ass as I sat here. Everything seems to work, and the physics all play out very nicely in my head, but I'll include this disclaimer nonetheless.
|This thread has more than 10 replies. Click here to review the whole thread.
|Posting Rules
You may post new threads
You may post replies
You may not post attachments
You may not edit your posts
BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


text:
Reds narrow GM search
The Reds expect to have a new general manager in place by Friday.
Whom that will be remains unknown. The original list of nine candidates has been trimmed to a select few who will be called back for a second interview.
Reds chief executive officer Bob Castellini was asked today how many names are on the list.
Its somewhere in the neighborhood (of two or three), Castellini said.
Wayne Krivsky, the Minnesota assistant general manager, and Jim Beattie, a Reds special advisor, are likely among the finalists. Both declined comment today.
Im going to let it come from (chief operating officer) John Allen, Krivsky said. Hes scheduling things.
Allen did not return a call this afternoon.
Krivsky was the choice of nearly everyone in the organization in 2003, when Dan OBrien got the job. Then-CEO Carl Lindner overruled his front office people and hired OBrien.
One candidate who wont be on the list of finalists is Philadelphia assistant GM Mike Arbuckle.
They called me and said they were going in a different direction, Arbuckle said this afternoon.
Two other candidates Atlanta assistant GM Frank Wren and Reds director of international scouting and player development Johnny Almaraz said they had not heard anything from the club since their interviews.
Other candidates:
Brad Kullman, the Reds interim GM
Leland Maddox, a Reds scout and former scouting director.
David Wilder, Chicago White Sox assistant GM.
John Mozeliak, St. Louis assistant GM.
Calls to Kullman, Maddox, Wilder and Mozeliak were not returned this afternoon.
Castellini said hes confident the Reds will get a good GM.
We felt like we had the best people available in, he said. We were very impressed with the candidates.


text:
Discussion in 'Great Skate Debate' started by lovesdance, Jan 4, 2012.
Has anyone bought the "movie rights"?
Thanks, Heather!!!! I'll get ready to be tackled!
That would be awesome! If any producers are reading this, please contact me. I already have some ideas for who I'd like to play the lead roles.
well, I want first right of refusal for the role of the coach!!!!!!!
Can you do a Russian accent? I'm not sure if Josh Dallas can, but he's my dream choice.
Another skating movie
I'm guesting on Mason Canyon's blog today, talking about the setting of the book.
Congratulations on getting your book published!
Next month, I'm going to do some Life on the Edge trivia contests on my blog with some fun prizes! I'll post the links here.
Finished reading the story and really enjoyed it, just sorry it ended so soon. Any chance of an sequel?
Awesome! Thank you! Tell all your friends I do have some ideas for a sequel! I'm going to start writing after I get past the January-February work and skating event crush
Shared it with my Twitter feed--lots of Kindle peeps on there!
Thank you!!!!
I'm at Jillian Jenkins' blog today, talking about the soundtrack to the book. Some Sting, Coldplay, Alicia Keys.....
As a special promotion for Nationals week, anyone who purchases the book between now and Monday, Jan. 30 and emails me the receipt (from Amazon, B&N, etc) will be entered into a drawing for a special autographed item from San Jose. My email address is [email protected].
Just got it for my iphone - can't wait to read it!
A little OT, I know, but whatever happened to all those serial fanfics like On the Edge and Rink Rat Rumors after that website closed? Are they archived anywhere else? I feel like a little stroll down skatefic memory lane.
Cool! Email me if you want to be in the drawing!
I'm extending the promotion through Feb. 6 since it's running on my blog and I didn't get my Nationals posts up timely. Enter for a chance to win a poster signed by Meryl Davis and Charlie White! Thanks to everyone who's already sent in their entries
This was a great, quick read. It sucked me in and I stayed up WAY too late last night to finish it!
I've had a few people email me in the wee hours to tell me they couldn't go to sleep before finishing! Sorry I kept you up but so glad you enjoyed it!
Holding another promotion on my blog - all you have to do is leave a comment for a chance to win an Amazon gift card.
Romancing the Valentine Giveaway Feb. 6 - 13
And some very exciting news - the paperback version of Life on the Edge should be available by the end of the month! I'll update when I get a firm release date on Amazon!
Can't wait for the paperback release!!
I have just one question: When is the sequel coming out?
I started writing it yesterday! Working title - Edges of the Past
If anyone who read the book would like to leave a review on Amazon or B&N, I would be so grateful. I'm always looking for more feedback!
Just finished it on my Kindle this morning on the subway ride to work. LOVED it!!! I'm a sucker for a good love story, especially one on ice Nice work, TanithandBenFan! Looking forward to the sequel!
Every Saturday I've been posting deleted scenes on my blog as part of the Sweet Saturday Sample series. Here is this week's entry.
The print copy of the book is currently in production!
I finally bought a kindle and your novel is the first one I downloaded. I started reading and will make sure to write a review later.
Thanks! I really appreciate it!
I just downloaded it and am looking forward to reading it.


text:
Live Nation - Special Events
Live Nation Las Vegas Boulevard South
Las Vegas, NV 89109 (702) 000-0000
Monday thru Sunday: Closed
Live Nation is a live-events company based in Beverly Hills, California, focused on concert promotions. Live Nation formed in 2005 as a spin-off from Clear Channel Communications, which then merged with Ticketmaster in 2010 to become Live Nation Entertainment. Live Nation "signs" artists as a "record label", but predominantly takes the role of a promoter, rather than "owner of music". The deal with U2 and Madonna for example does not include copyright of the artists' future recordings. The 2007 deal signed with Jay-Z does include the rapper's future recordings. Live Nation signed Colombian singer Shakira, Canadian band Nickelback and Mexican singer Luis Miguel.
- No upcoming events have been scheduled.
Average scores for sexiness, atmosphere, lines, prices, dancibility, music and more.
Check back here often to view user reviews and ratings. Better yet, visit Live Nation - Special Events and post your own review and rate their performance!
Real time stream from our members with confirmed users at local venues!
Reggie C. commented on Lisa Lampanelli


text:
Sweet 'n' Spicy Halloween Munch RecipeRead Reviews Be the first to add a review
4.5 1 2
Kids of all ages love the sweet and salty blend in this fast-to-fix snack mix. —Shana Reiley, Theresa, New York
Featured In: 25 Fun Halloween Treats
- 1 pound spiced gumdrops
- 1 pound candy corn
- 1 can (16 ounces) salted peanuts
- In a large bowl, combine the gumdrops, candy corn and peanuts. Store in an airtight container. Yield: 2 quarts.
Originally published as Sweet 'n' Spicy Halloween Munch in Taste of Home's Holiday & Celebrations Cookbook Annual 2003, p240
Reviews for Sweet 'n' Spicy Halloween Munch
Please Log In or Join to add a rating and review.
Click stars to rate
Any changes to your rating or review will appear where you originally posted your review
Reviewed Dec. 9, 2010
"This was my recipe (Shana Reiley) which was published in Taste of Home's Holiday & Celebrations Cookbook in 2003. I love how easy this is to make and there are so many ways to present this simple treat."


text:
'The Sabres didn't help themselves at the start taking three penalties within the first four minutes resulting in two 5 on 3 power plays for Toronto. The Leafs scored on both two-man advantages, the first coming from Darcy Tucker and the second just a minute and a half later Kyle Wellwood would also score giving the Leafs an early 2-0 lead.'
Sabres Finish Off Leafs 7-4
November 23, 2006 Submitted by
Via: WGR-AM


text:
Ethics: Theory & Practice
Catalog number: PHIx310
Description: Corresponds to a one-semester course in applied ethics. Measures understanding of ethical knowledge as it applies to practical ethical situations. Application of knowledge about ethics is accomplished through the use of case studies and related sets of multiple-choice questions. Knowledge categories include basic theories and concepts such as utilitarianism, natural law theory, justice, duties and obligations, and rights; metaethics, covering topics such as subjectivism, objectivism, and naturalistic fallacy; and moral deliberation, covering topics such as moral sensitivity, status of moral judgments, and implications of moral concepts. Knowledge from these categories is then applied to practical ethical issues such as social and personal issues, bioethical and medical issues, professional and business issues, and environmental issues. Assumes a familiarity with the content generally taught in an introductory ethics course. Multiple-choice examination.
Exam Type: Multiple Choice
Credit / Level: 3.0 / Upper
Exam Fee: $355*
* Fee shown is for the exam only. Other associated expenses such as books and study resources, or travel, lodging, or providing any required documentation or background checks, are additional.
Find recommended books and study materials.
Exam Content and Study Guides for Ethics: Theory & Practice
Practice Exam: A practice exam is available for $75 - to register for a practice exam, you must first register for its corresponding credit-bearing exam.
Excelsior College Test Administration
7 Columbia Circle
Albany, NY 12203-5159
+1-518-464-8777 fax
Test Preparation Advisory
Preparation for Excelsior College® Examinations, though based on independent study, is supported by Excelsior College with a comprehensive set of exam learning resources and services designed to help you succeed.
These learning resources are prepared by Excelsior College so you can be assured that they are current and cover the content you are expected to master for the exams. These resources, and your desire to learn, are usually all that you will need to succeed.
There are test-preparation companies that will offer to help you study for our examinations. Some may imply a relationship with Excelsior College and/or make claims that their products and services are all that you need to prepare for our examinations.
Excelsior College is not affiliated with any test preparation firm and does not endorse the products or services of these companies. No test preparation vendor is authorized to provide admissions counseling or academic advising services, or to collect any payments, on behalf of Excelsior College. Excelsior College does not send authorized representatives to a student's home.
Depending upon your individual learning style and needs, you may consider using the services and products of a test preparation vendor to help you prepare for an Excelsior College Examination. Some students have found such materials beneficial while others have not. The decision to purchase the services and products of any of these vendors is entirely up to you.
Excelsior College does not review the materials provided by test preparation companies for content or compatibility with Excelsior College Examinations.
To help you become a well-informed consumer we suggest that you consider the following before making any purchase decision regarding study materials provided by organizations other than Excelsior College.
Does the company:
- Clearly articulate that it is an independent business enterprise, separate from Excelsior College, and that it is not a degree granting institution?
- Clearly inform you that the products and services it provides have not been reviewed, nor are they endorsed, by Excelsior College?
- Openly indicate that it is not an agent of Excelsior College, that it is not authorized to provide admissions counseling or academic advising regarding Excelsior's degree requirements, and that all its costs and fees are separate from those you will pay to the College?
- Have a record of unresolved complaints/disputes with consumer protection agencies, nursing boards, attorneys general offices, or other similar organizations?
- Require clients to commit to long-term, binding contracts to access its products and services?
- Provide data to substantiate the satisfaction of its clients with its products and services?
We have been made aware of several recent incidents in which a test-preparation firm has contacted an Excelsior College student requesting access to their Excelsior College Examinations ® preparatory materials, including practice exams. Sharing learning resources with a test-preparation firm is a clear violation of the academic honesty code. Students found to have engaged in academic dishonesty at Excelsior College will be subject to disciplinary action.
If you are approached by any individual or third-party about sharing any Excelsior College provided study materials, please contact us at PR (at) excelsior.edu.
Exam Registration
First, create your own free MyExcelsior account. Once you have created an account, you can register online for an exam. You may also register by calling toll free, 888-647-2388 ext. 166.


text:
|Topic Review (Newest First)
yours are the reflex ones, right?
I have the limiteds and the gas pedal works exactly the same as on regular reflex cartels, genesis, malavita etc.
I was planning on moving a pair to my new juice wagon so I could take a photo for you if you want
|I have these...they're like the new malavita,genesis,and diode base plates....sorry I'm too lazy to take pics,but pull up on the two tabs and bend the foam flap over. Now get a firm grip on that foam piece that includes the flap by placing your 4 fingers where the disc goes and pull outwards towards the toes. It takes some force and you may need to wiggle a bit.
Well I don't know what to say, with the bindings off it isn't clear, and according to Burton customer support it can't be done (even though according to the instruction booklet you can - but like I said they probably package the same book with every binding).
If anyone has clear instructions or a picture or a video for the 2013 Cartel Limited (some places list them as 2014 early release) I'd like to see it, but I don't want to start peeling up foam and ruining the binding if they aren't actually adjustable.
With the binding off of the board, it should be pretty clear.
Turn the binding upside down so you're looking at the bottom. Look at the toe/gas pedal part of the footbed from the bottom. Are there little plastic tabs coming through from the top with a catch/barb/snap thing? Maybe 4 of them. If so that is what latches the top of the gas pedal to the plastic bottom. These two parts encompass the front of the baseplate. These two parts together slide forward/backward on the baseplate so you can adjust the footbed length.
Heck, if you unsnap the rear of the footbed with the bindings off, the gas pedal should slide freely in and out. Then you can see how it works.
If you'res doesn't look/work like this, then i guess they're different.
I have the re:flex ones shown here: http://www.burton.com/mens-cartel-sn...efault,pd.html
People are getting confused because the Cartels have come with a bunch of different base plates and foot beds over time. For instance, the 2012-13 Limited/2013-14 ones have the Genesis/Malavita base plate which is different from the other 2012-13 Cartels.
So it is important to be very specific about which binding we are talking about.
Yeah there is just one piece of foam that goes across the entire footbed on the Limiteds. You have to pop the snaps by the heel (I think those are the ones you're talking about?) to get to the disc. The foam just bends and creases the first time you flip it up. You can get at them from the top, but they didn't free the gas pedal when undone.
There are instructions, but they're useless. They probably package the same little booklet with every binding they sell; the "gas pedal adjustment" instructions are a picture of the bindings with an arrow pointing to the gas pedal :/
Originally Posted by goodbyegalaxy View Post
Hm, maybe just the 2013 Limiteds (and potentially the 2014s?) then? There are no tabs on mine to be seen, and there is foam on the top and the bottom of the binding. I asked customer support if I was supposed to peel back the foam to find the tabs and they said not to.
Are there no instructions in the box they came in? Not sarcastic,.. asking?
Later edit: WTF, Maybe the limiteds are one piece foam! IDK? I have the 2012/13 Restricted Cartel's! If they are? How the fuck did burton expect you to get at the disks to get them mounted?
Burton may want you to "pop" them off the snaps/tabs whatever the everlovin F they call em from the bottom of the binding, course, you wont be able to do that once they're mounted! So tell burton that they BETTER be able to be peeled back from the top!!! Either that or they're meant to be permanent?
|Hm, maybe just the 2013 Limiteds (and potentially the 2014s?) then? There are no tabs on mine to be seen, and there is foam on the top and the bottom of the binding. I asked customer support if I was supposed to peel back the foam to find the tabs and they said not to.
|That's not true. At least with the 2013 Cartels. The toe part of the gas pedal pulls apart from the bottom. There are little plastic tabs that latch. You can pop the top off, slide the bottom in and out to adjust length. Then pop the toe back on and snap the rear into the slot that lines up.
Originally Posted by BiG NicK View Post
Yeah youtube didn't really help for this year's bindings for some reason... just reviews on it. I figured it out, and you just have to really yank on the front toe section outward and it eventually extends (no unscrewing needed, just pop out the 2 side heel clips). And for anyone viewing this with the same problem, just fold up the padding, it doesn't detach completely.
After reading this I yanked and yanked on the gas pedal/toe ramp, and it wouldn't slide out at all. I emailed Burton and this was their response:
Yes, the FullBED pads will not be adjustable in length. Hopefully your boots fit well in the binding and there isn't the need for an adjustment.
|This thread has more than 10 replies. Click here to review the whole thread.
|Posting Rules
You may post new threads
You may post replies
You may not post attachments
You may not edit your posts
BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


text:
A bill that would nullify future federal attempts to control firearms easily cleared the Senate but not without plenty of fireworks. Senate Bill 129 prohibits the enforceability of any new federal law, rule, regulation, or order relating to the ownership or registration of certain firearms, magazines, or accessories. Bill opponents cast the bill as an attack on the Constitution. But the sponsor, Jared Carpenter of Madison County, said the measure is in response to the Obama administration's "assault on the right to bear arms."
Carpenter, a Republican, credited Democrat Ray Jones, a Pike County lawyer, for helping to draft the bill. Jones was the first of five lawyers to speak on the bill. The debate turned largely on the Supremacy Clause of the U.S. Constitution, which holds that federal law trumps state law where conflicts arise. Jones mentioned the Supremacy Clause in a floor speech.
Senator Kathy Stein, a Lexington attorney, continued Jones' theme to argue that states can't pick and choose which federal laws they will and won't enforce.
She said she's not an enemy of the 2nd Amendment, but asserted there are reasonable limitations of Constitutional rights.
Finally, attorney Gerald Neal of Jefferson County defended the federal government and expressed disappointment that lawmakers would vote to nullify the Supremacy Clause. He reminded his colleagues that the business of making policy is serious and no place for games.
Senate Bill 129 cleared the chamber by a vote of 34 to 3. It now awaits assignment to a House committee.
There was deeper partisan division over another measure the Senate approved. Chris McDaniel's Senate Bill 55 would let voters decide whether to synchronize the election of state officers with the presidential election cycle beginning in 2016. The proposed Constitutional Amendment would add a year to the terms of current constitutional officers including the governor. The measure passed the Senate 25 to 12 along partisan lines and now advances to the House.
In Kentucky House action….
House Speaker Greg Stumbo says drug overdose deaths have declined, narcotics prescriptions are down, and so are costs thanks to hallmark legislation passed last year meant to shutter unscrupulous pill mill operations that were exacerbating prescription drug abuse throughout the state.
He hailed House Bill One of 2012 as a dramatic and bold step to crack down on pain management clinics with loose prescription practices, but one that that yielded some unintended consequences and complaints from providers who believe it's overreaching. The Prestonsburg Democrat explained that the tweaks offered in his House Bill 217 are not a retreat from the new law, but mild adjustments to make it work better as he explained to the House Judiciary committee Monday afternoon.
House Speaker Greg Stumbo's House Bill 217 cleared the House judiciary committee without opposition. It now advances to the House for a vote by the entire membership.
Thanks to Steve Shaw, our Senate reporter, for contributing to this post. Watch "Legislative Update" each weeknight at 11pm ET for a daily digest of committee and chamber actions. Follow @ReneeKET on Twitter throughout the day for updates.


text:
A Rare American Oratorio to be Heard, Uninterrupted
When WQXR and NPR Music present the Cincinnati Symphony Orchestra and May Festival Chorus's performance of R. Nathaniel Dett's The Ordering of Moses at Carnegie Hall Friday, there will be one significant difference from its first airing: it should be free of interruptions.
Just why the 50-minute oratorio was not heard in full on NBC radio in 1937 is a story steeped in allegations of racial censorship. Dett (1882-1943) was a black Canadian-born American composer who earned degrees at the Oberlin Conservatory and the Eastman School of Music. He later studied in Paris with Nadia Boulanger, the revered teacher of composers from Aaron Copland to Philip Glass. Dett called his mission "the emancipation of Negro music" and cited Dvorak's promotion of black spirituals and folk songs as a strong influence on his own works.
The Cincinnati May Festival commissioned Dett to write The Ordering of Moses. He used a text based on Exodus, fusing biblical narrative with spirituals to capture the idea of freedom from bondage.
In NBC's live broadcast, only 40 minutes of the work aired. Near the end of the original acetate disc, the announcer can be heard saying, "We are sorry indeed, ladies and gentlemen, but due to previous commitments, we are unable to remain for the closing moments of this excellent performance." Some organ music filled out the hour (listen to that moment at the top of this page).
"It's very clear from the program that was published, it was meant to be a full hour and a full piece," said James Conlon, the May Festival music director, who will conduct this performance at the Spring for Music festival. "So it's very clear that something happened." Historians suspect the broadcast was cut short after racist callers to the network objected.
The Cincinnati May Festival revived Dett's oratorio in 1956, but it has been rarely heard since. After a 1993 performance at the American Academy and Institute of Arts and Letters in New York, Alex Ross wrote in the New York Times that the piece suffered from an unwieldy structure and some meandering moments, but added, "there are episodes of startling power."
This concert will begin with John Adams's Harmonium, a 1980 piece based on poetry by John Donne ("Negative Love") and Emily Dickinson ("Because I could not stop for Death," "Wild Nights") that is regarded as milestone of Adams's minimalist period. The texts, an essay by the composer and an excerpt may be found here.
Conlon, who has promoted composers suppressed by the Nazis, sees this Dett performance as an extension of that rescue mission. "If any of us artists hear music that has a value and for whatever reason is not played, I'd rather do that than the umpteenth performance of Beethoven or Tchaikovsky," he says, adding that there's nothing wrong with those composers. "This was an ideal opportunity to do that in the context of Spring for Music."


text:
Football historian LEIGH EDWARDS profiles every Arsenal player from 60 post-war League seasons.
Young winger Tommy Black made his solitary Premiership appearance as a substitute in Arsenal's 1-0 win at Everton in April 2000. A former England Schoolboy trialist, he followed his older brother Michael through the ranks at Highbury and turned professional in July 1998. His only other first-team outing was in the Gunners' 1999/2000 Worthington Cup Fourth Round defeat at Middlesbrough and he was loaned to Carlisle United and Bristol City before joining Crystal Palace for £250,000 in July 2000. He featured in their 2003/04 promotion campaign and has since had loan spells at Sheffield United and Gillingham.
England international central defender Jeff Blockley helped Arsenal finish League Championship runners-up in 1972/73. Signing professional for Coventry City in June 1967, he skippered their 1968 FA Youth Cup final side and starred as Sky Blues qualified for the Fairs Cup in 1969/70. He became captain and moved to Arsenal for £200,000 in October 1972, gaining his solitary England cap against Yugoslavia a week later. Scoring once in 52 First Division games, he was sold to Leicester City for £100,000 in January 1975, then played for Notts County, Enderby and Shepshed Charterhouse. He now runs Transmech Ltd in Nuneaton.
England Under-23 inside-forward Jimmy Bloomfield was ever-present for Arsenal in 1956/57. Initially with Hayes, he joined Brentford in October 1952 and moved to Highbury for £10,000 in July 1954. He represented the Football League and netted 54 goals in 210 First Division games for the Gunners, joining Birmingham City for £30,000 in November 1960. Helping them win the League Cup in 1963, he had spells with Brentford again, West Ham and Plymouth before becoming Orient's player-boss in March 1968. He took charge of Leicester City in June 1971, then managed Orient again and died in Chingford in April 1983.
Portuguese international winger Luis Boa Morte helped Arsenal to win the 'double' in 1997/98. Previously with Sporting Lisbon, he joined the Gunners in June 1997 and his pace was a constant threat to defences. He appeared in Arsenal's 1998 and 1999 FA Charity Shield successes and regularly featured as a substitute, playing 25 Premiership games before being sold to Southampton for £500,000 in August 1999. Joining Fulham for a reported £1,700,000 in July 2000, he starred in their 2000/01 First Division title triumph and 2003/04 FA Cup run. He was a member of Portugal's squad that reached the 2006 World Cup semi-finals.
Ex-England Schoolboy wing-half Micky Boot scored on his league debut in Arsenal's 2-0 win at home to Newcastle United in October 1966. Signing professional at Highbury in December 1964, he featured in two FA Youth Cup finals including the Gunners' 1966 success and netted twice in four First Division games prior to joining Port Elizabeth City in March 1967. Later with Nuneaton, Kidderminster and Leamington, he played for Australian sides Townsville, Mareeba and Phoenix before coaching Lake MacQuarie. He has worked for British Oxygen, Fosters Wine Estates and now Port Waratah Coal Services in Newcastle (NSW).
England international central defender Steve Bould was an experienced figure in Arsenal's 1997/98 'double' success. Signing professional for Stoke City in November 1980, he returned from a loan spell at Torquay United to secure a regular first-team slot and followed Lee Dixon to Highbury for £390,000 in June 1988. He also helped Arsenal win the League Championship in 1988/89 and 1990/91, then the FA?Cup and League Cup in 1993 and ECWC in 1994, scoring five times in 287 league outings for the Gunners before moving to Sunderland for £500,000 in July 1999. A persistent toe injury ended his playing career and he is now coaching at Arsenal's Academy.
Welsh international wing-half Dave Bowen was captain of club and country whilst at Arsenal, featuring in the 1958 World Cup. Signing professional for Northampton Town in July 1947, he moved to Highbury for £1,000 in July 1950 and understudied Joe Mercer as Arsenal won the League Championship in 1952/53. He scored twice in 146 First Division games for the Gunners before returning to Northampton as player-manager for £5,000 in July 1959, plotting their remarkable rise to the top-flight. Also managing the Welsh national side for seven years, he later became Northampton's secretary and died in September 1995.
Republic of Ireland international midfielder Liam Brady was capped 26 times whilst at Arsenal. Signing professional at Highbury in August 1973, 'Chippy' was ever-present in 1975/76 and starred in three successive FA Cup finals, including the 1979 Wembley triumph when voted PFA Player of the Year. He also featured in the 1980 ECWC final and netted 43 goals in 235 First Division games for the Gunners before moving to Juventus in July 1980. Later playing for Sampdoria, Inter Milan, Ascoli and West Ham, he managed Celtic and Brighton before rejoining Arsenal as head of youth development in August 1996.
Teenage central defender Steve Brignall was a young member of Terry Neill's squad at Arsenal. A former apprentice at Highbury, he turned professional in May 1978 and made his sole First Division appearance as substitute in Arsenal's 3-0 defeat at Liverpool in April 1979. He joined Norwegian side Harstad with Cliff Cant in December 1979, then had a trial with Watford before playing for Hastings United, Tonbridge, Gravesend & Northfleet, Tenterden, Ashford Town, Hythe Town and Ashford again. Still living in Ashford, he is a partner in S & A Builders and currently manages Bethersden in the Canterbury League.
Ex-England Amateur international Laurie Brown missed just one match for Arsenal during the 1961/62 campaign. He starred for Bishop Auckland and represented Great Britain in the Rome Olympics shortly before joining Northampton Town in October 1960. Top scorer in their 1960/61 promotion success, he moved to Arsenal for £35,000 in August 1961 and netted twice in 101 First Division games prior to joining Spurs for £40,000 in February 1964. He also played for Norwich, then managed Bradford PA, King's Lynn, Altrincham, Stockton and Tow Law. Later a publican and milkman, he died in Aycliffe in September 1998.
Young goalkeeper Tony Burns contested a first-team slot with Jim Furnell and Bob Wilson whilst at Arsenal. Initially with Tonbridge, he moved to Highbury in March 1963 and was given his League debut in the Gunners' 3-2 win at home to Burnley in October 1964. He made 31 First Division appearances prior to joining Brighton for £2,000 in July 1966, then played for Charlton Athletic, Tonbridge again, Durban City, Crystal Palace, Brentford, Memphis Rogues, Plymouth Argyle, Dartford and Hastings United. Later managing Tonbridge (twice) and Gravesend & Northfleet, he is currently Millwall's goalkeeping coach.
Young Argentinian striker Fabian Caballero's only Premiership appearance was as a substitute in Arsenal's 1-1 draw at home to Middlesbrough in November 1998. He impressed with Serro Portino in Paraguay before moving to Highbury on loan in October 1998, also featuring in the Gunners' 5-0 Worthington Cup fourth round defeat at home to Chelsea and 4-2 FA Cup third round victory at Preston. After playing back in Paraguay for Atletico Tembetary and Sol de America, he joined Dundee in July 2000 and featured in the 2003 Scottish Cup final. He has since returned to Paraguay again and is currently playing for Tacuary.
England U-21 defender Gus Caesar played for Arsenal in the 1988 Littlewoods Cup final. Signing professional at Highbury in February 1984, he made his league debut in Arsenal's 1-0 win at Manchester United in December 1985. He provided reliable defensive cover and was a member of the Gunners' 1988/89 League Championship squad. Making 44 First Division appearances prior to joining Cambridge United in July 1991, he then played for Bristol City, Airdrie, Colchester, Dagenham & Redbridge and Hong Kong sides Eastern, Golden, Sing Tao and Hong Kong Rangers. He is now a financial consultant in Hong Kong.
Copyright 2017 The Arsenal Football Club plc. Permission to use quotations from this article is granted subject to appropriate credit being given to www.arsenal.com as the source


text:
Some people may know a little of me in these forums as I am very much an ideas guy when it comes to modelling, I have for a while now caught the bug when it comes to prop modelling, but for me it's really a case of a hobby that hopefully I can make a little money on.
I am a graphic designer but I also did a lot of modelling when I did a product design course.
So I started making my own Boba Fett Helmet, but, as I began researching it I began to realise that there was a market for Jango too. Anyhoo, I began making my own helmet based around a cycle helmet.
It was only recently that I discovered TDH and it was then I realised that people can be really pedantic about detail here, which was kind of demoralising for an amateur modeller trying to give to the community, so I had to make the decision that I would build my helmets for people who either aren't that bothered about replicating the wrong angles and brow furrows or just want a standard helmet that they can easily customise.
Problem is I am a little bit of a perfectionist and I am still working out the kinks for close up detail (I have to have the cheeks without any dinks in them so they are perfect for casting).
Anyhoo, this is my master which I am showing to you as the community of Mando variants type people as you guys seem really friendly and share more of my attitude when it comes to costuming. I intend to do them in fiberglass which will make them light and easy to work with, I can probably stick some aluminium in the gelcoat if needed. The ear pieces were bolted on a long time before I made the decision to make a generic helmet instead of the Boba one, but a dremel would soon sort that out should anyone wish to convert them.
Once I have the basic cast done, I will be making limited runs of "art" pieces, which will be my own customisations that turn the helmet into a decorative art form and I have a few ideas I want to run with, I'll pop my designs down here once I have the basics done, don't want to run before I walk
Any comments would be gratefully accepted and I'll keep you updated.


text:
|Topic Review (Newest First)
|good information on the rear end.....
It is used on street rods because of the width and it is a drop center 8"type rear. Lots of custom gears available for it. It looks good. Tho, now it is getting hard to find.
The Mustang rear loader didn't get popular with the street rod people because it is hard to set up, it's ugly and sometimes a bit pricy on initial cost.
Now street rod builders are using the S-Truck rears. CHEAP, and gears and parts are everywhere . Also rears from early 90's T-bird/Cougar Fords are good if you want a simple independant rear suspension. They remove from the donor car with six bolts and are very adaptable to older frames. They give you disc brakes, Independant rear suspension (good ride). Tho the gears are a bit non performance. Aftermkt gears are available. They are also a good looking setup.
Lincoln Versailles
i was just wondering , why do most rodders and custom guys use the rear end from the older lincolns versailles on rods and alot of impalas . i know the rear has disk brakes..you can run skirts with no problem.on an impala . but what are the spes... gears..( i think its 2.50:1 ratio).and also Parts for these rear ends are therefore becoming scarce, and the parts that usually need to be replaced often in this swap (rotors, calipers, soft lines) are becoming rather expensive.whats the lenght of the rear......... but why cant you use a rear end off a car like the 1994 and up mustangs..the lincoln it 1/2 longer then the mustang.....the mustang has rear disk brakes also.. you can just replace the bracket from an impala on to that rear... just a question... any help would be great....
or what other rear end do people use....
what are the pros and cons...to using that rear end...
|Posting Rules
You may post new threads
You may post replies
You may not post attachments
You may not edit your posts
BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


text:
Imaginative Ways of Displaying Your Kids' Artwork
15 comments:
- Nova Joy said...
i love the idea in your living room, looks very relaxing and comfortable for everyone to stay and hang out.
- March 4, 2014 at 3:50 AM
- Ako Si Carmel said...
I love minimalist style of decorating! Thanks for sharing some tips, it's useful :-)
- March 4, 2014 at 4:13 AM
- Mhie Lee said...
You have a nice living room. I prefer modern with an industrial touch. Thank you for sharing your great ideas.
- March 4, 2014 at 6:23 AM
- mavic cantes said...
Nice tips. In this way, kids would really develope more on their skills and be more creative. Thanks for the tips :D
- March 4, 2014 at 6:40 AM
- Juliana said...
I admire solid lines in minimalist designs, fuss free and clean.
I'm a traditionalist by heart however. But nowadays, eclectic rooms are popping up. Combination of two styles in a room if executed properly looks good and elegant :-)
- March 4, 2014 at 7:00 AM
- novemberletters said...
hmmmm great ideas, I think I will go for a minimalist style too when we get to renovate our home sweet home...
- March 4, 2014 at 9:30 AM
- jo-anne said...
Agree, no more bulky furniture. These type of design makes the room look bigger and clean. lol
- March 4, 2014 at 12:03 PM
- Betchai said...
i am a minimalist too, i simply don't like over crowded setting, and i fall in love right away with your design, so so roomy, spacious, simple but elegant and classy.
- March 4, 2014 at 12:27 PM
- Che Syjongtian C said...
if only my husband is not a keeper of too many things with sentimental value, i would love to incorporate minimalist living in our home. :D
- March 4, 2014 at 1:35 PM
- chubskulit said...
When my kids are a bit older, I would go for a minimalist style.
- March 4, 2014 at 10:15 PM
- Jessica Cassidy said...
I like that design and space too. You remind us to redecorate our living room. My hubby said that we should do it this year :-)
- March 4, 2014 at 11:51 PM
- Ria C said...
I like minimalist style. I prefer the uncluttered and very organized look in my home. That living room arrangement is very pretty. I want to copy that. :)
Ria C
- March 5, 2014 at 1:39 AM
- marrimye said...
It's the IN thing today. Minimalist interior is simple yet elegant. I don't want my walls look busy as well as my cabinet fixtures.
- March 5, 2014 at 4:52 PM
- lencilicious said...
I love room just like that. I hate seeing things not in proper order or scattered all around.
- March 5, 2014 at 11:39 PM
- Rcel said...
I like minimalist designs, too. Spic and span looking! No to furniture made of glass though, now that I still have super active kiddos. You have a nice-looking living room! :)
- March 6, 2014 at 1:36 AM
Notepad Blogger Design by blankPixels | Original CSS layout by Gisele


text:
Our mission: design a shopping cart cover that you can truly put on while holding a squirmy baby! Our streamlined cover shields just as well as big, flouncy ones, but is a dream to handle—you can really put it on (and take it off) with just one hand. Adjusts to fit all carts. Nicely padded for baby's comfort, with side pockets, toy loops, harness, and carry bag. Machine wash. Imported. Designed by OneStepAhead!For more information, click here.
Sign in to your Address Book.
To check if any specific restrictions for this item apply to any United States shipping address, please enter the information below.Sorry, this service is not available to Canadian addresses.
This is being shipped to a P.O. Box
This product has no shipping restrictions.
© 2017 One Step Ahead


text:
Information For...
FAA. Admission of International Students (BP)
FAA. Admission of International Students (BP)
Recognizing that the college community is part of a global society and that one of the purposes of higher education is to facilitate a better understanding of the peoples, cultures and nations that shape our world, it is the belief of the Board that students from other countries should be encouraged to attend South Plains College. Therefore, such students, who meet international admission requirements, shall be encouraged to attend South Plains College, and shall be admitted without regard to nationality, place of birth, residence, race, color, national origin, religion, gender, disability or age.
At a minimum, International students must:
- Meet general admission requirements outlined for high school graduates or college transfer students.
- Demonstrate acceptable proficiency in the English language;
- Submit proof of financial ability.
Additional requirements for the admission of International students may be established by the Dean of Admissions and Records. Specific policies and procedures governing the College's policy for the admission of international students shall be published in the current General Catalog.


text:
- Home
- News
- Sports
- Opinion
- Obituaries
- Features
- Special Sections
- Classifieds
- Public Notices
Like most children who grew up small Kentucky towns, my earliest holiday memories are of family, presents and lots of good food. My mother and both grandmothers were great cooks and meals around the big dining room table were part of our family's holiday tradition.
But, in particular, there was something special about the fruit salad.
To a 6-year-old, it was simply the largest bowl of fruit in the world, overflowing with harvest from such exotic lands as South America, Africa and even California. It was a succulent masterpiece and it was created each year by my Grandfather Ely as his contribution to our annual Christmas feast.
As the oldest grandchild, I was the first to be invited into one of the most hallowed places of the holiday season – my Grandmother Ely's kitchen – to witness firsthand the preparation of the much-anticipated dish. It was there, amidst the aroma of turkey roasting, fresh blackberry cobbler cooling and sweet potatoes simmering in butter and brown sugar that all the ingredients were brought together.
I still can see my grandfather sitting at the kitchen table, carefully cutting red and yellow apples, ripe bananas, juicy oranges, tart grapefruit and fresh, sweet pineapples. Freshly cracked pecans, cherries, white grapes, an assortment of berries, plump dates and his own special ingredients also were prepared. Then, he carefully mixed everything together until it was pronounced finished and ready to eat.
While he peeled, cut and mixed the fresh fruit, he and I would ponder over things that mattered to grandfathers and their grandsons – baseball, bicycles and girls. He also would reminisce about his own childhood in the country, tell funny stories about family members and always remind me about why we celebrated Christmas.
One holiday season, early in my teenage years, I found it less interesting to join him at the kitchen table. Of course, my grandfather continued his labor of love with my younger sister or cousins occupying the chair of honor beside him. He passed away in 1970 and, while the family dinners continued, his traditional gift to us became a treasured memory.
As the years passed, I graduated from high school, completed college and graduate school, got married and had children of my own. Together, we established our own family traditions.
But each holiday season, I always remember my grandfather's Christmas fruit salad.
For several years, I talked about it with my wife and children and they encouraged me to recreate it for them. Naturally, there never was a written recipe and my efforts always fell short of expectations.
As I've grown older, I've thought a lot about my grandfather and his fruit salad. Once, it occurred to me that while everyone enjoyed eating it, I never saw my grandfather dish out a single bowlful for himself.
The reason was simple enough. You see, he was diabetic. I guess that fruit salad, with all its sweet, delicious goodies, was just not on his restricted diet.
Another time, I realized I really missed my grandfather more than I did his fruit salad. His holiday gift to his family had been a wonderfully delicious concoction; his gift to me during all those holidays had been much more.
My wife reminds me that an enduring lesson of the Christmas fruit salad is that family and family traditions, are what really matter in our lives. The memories they create help keep our loved ones alive in our hearts and minds long after they are gone and help bring them to life for the younger generations. As always, she's right.
Recently, I shared memories of the Christmas fruit salad with a friend of mine. This wise man noted my grandfather's fruit salad was much like our community: made up of many different ingredients, each from a different place and with its own, distinctive flavor. Mixed together, the individual ingredients complement each other and become part of something very special.
I really liked that. My grandfather would have liked that, too. So, I've added it to my story for all of us to think about.
This holiday season, as you spend time and exchange gifts with family and friends, our family hopes your celebrations will become joyful new traditions for you and yours. And we hope that you will take time to remember and celebrate the birth of the Christ Child, the most wonderful gift of all.
Merry Christmas!
Ron Harrell is chief institutional advancement officer at Elizabethtown Communityand Technical College.
Add new comment
Read and share your thoughts on this story


text:
Greetings ^^ Well, I've been passing the time lately with some mmos (and SMT:Nocturne :3). I play with a friend and he wants to just (finally) stick with one and play it. Going to be truthful, I tend to game hop quite the bit, with only 3-4 mmos in my time playing them having kept me interested for more than 2 weeks-1 month.
But nonetheless, I've played many, many mmos, wouldn't be surprised if I've played most f2ps out there.
Anyway, we're just trying to find one that will pass for a semi-longer term (if not permanent) game, since I may be able to talk him into playing FFXIV:ARR when it is finished.
We're currently playing Eden Eternal (more so me) but I get that icky p2w feeling, considering I spent 10$ and got those beginner packages, after reading around a bit and experiencing a bit of the CS firsthand, it has a whole, good now bad later thing going on.
^ You can really skip most of that
Actual criteria
Anyway, we're just trying to find something to keep us entertained, I don't have many criteria just...
A Healthy population, I want to see the forums having some activity the chat boxes in game with it as well
Diverse class choice. :X When I look up classes and see Tank/Mage/Cleric/Ranger, I just leave the site, unless of course there are branching advancements.
Not P2W, don't get me wrong I'm fine spending money a game that I enjoy, but when I feel that I NEED to to advance it just makes me not want to play. If the only way to make money is via the cs, or the only way to get X armor/skill etc, etc, I'll pass.
I was thinking of trying out Rusty Hearts/Dragon Nest/Dungeon Fighter again, but for some reason I find that instanced action games are more fun (with usually more unique classes), they dry up a lot quicker, where as the more grindy ones like EE/Flyff etc tend to entertain for more time.
The games I actually stuck with: Final Fantasy XI(years), Mabinogi(years), Grand Chase(years). Most everything else was less than 2 months all together I'd say, granted the games above I/we played on and off, but for far longer intervals and over the course of quite awhile.
Any suggestions are welcomed, considering that I've probably played a good amount of them, but not for any real significant amount of time if I could ask you to just give a slight tidbit of info on why it is enjoyable.
I sincerely apologize for posting so much, but I just thought I should get the situation out, whilst I risk wasting your time. I
thank you kindly for taking the time to give me some assistance, it is much appreciated ^^.


text:
By: William Whidden, PE
Keeping up with the latest advancements in technology, materials, and construction is critical to maintaining the continued transport of safe drinking water and the reliable collection and treatment of waste-water. For this reason, the American Society of Civil Engineers (ASCE) presents an updated manual of practice (MOP) specifically for flexible steel pipe.
Released in August 2009, Manual of Practice 119, Buried Flexible Steel Pipe: Design and Structural Analysis was prepared by the society's Buried Flexible (Steel) Pipe Load Stability Criteria and Design Task Committee with input from a joint committee of the Steel Tank Institute and the Steel Plate Fabricators Association. The technical publication is structured to guide the reader, whether an engineering student or design professional, through the design or analysis process. Read on for a preview of topics discussed.
What precisely constitutes flexible pipe has long been the subject of some confusion. Complicating the situation further, terms such as "flexible," "semi-flexible," "semi-rigid," and "rigid" have been used.
Essentially, engineers can choose from two basic philosophies of pipe design: rigid and flexible. What distinguishes the two is the method of analyzing resistance to internal and external forces.
In rigid pipe design, the forces are additive: Internal and external loadings must be analyzed in combination as part of an evaluation of the stress in the pipe wall created by both thrust and bending forces. The wall of a rigid pipe is then structurally designed to resist these forces.
In flexible steel pipe design, internal and external pressures are evaluated independently because a combined analysis would show a reduction of the stress to be resisted. Therefore, independent analyses produce a more conservative design for flexible pipe than would a combined stress analysis. Flexible pipe deflects and conforms with soil embedment as soil is compressed, therefore flexible pipe wall should be designed to resist excessive deflection and buckling.
Designers of steel pipe must consider more than simply the thickness of the steel cylinder. For example, the type of coating and linings to be applied and the type of joint configuration to be used also must be considered. Simply stated, certain coatings and linings that work well in some installation conditions may not work well in others. The same holds true for the different joint configurations.
A contemporary practice manual may seem like an odd place for a history lesson, but engineers can benefit from understanding the role that pipelines have played throughout history. Knowing how design and construction techniques have evolved in response to differing circumstances can enliven and improve efforts to install new pipelines today.
Consider bamboo pipes in China. Clay pipes in Mediterranean countries. Rock-lined tunnels in Persia. These approaches were used to transport drinking water in the ancient world, sometimes across distances that remain impressive today.
Although iron had been used for making weapons and other implements for thousands of years, it was not used to make pipes until the 19th century. Stronger but costlier than other pipe materials, iron first was used for gas pipes in England. Within decades, inventors had devised methods for making steel in large quantities, and the era of the steel pipeline began. The impact of this innovation was felt most in cities, where growing populations benefited from increasingly sophisticated distribution systems.
First made in New York in 1905, lock-bar pipe was the next innovation. So named because it consisted of two semicircular pipe halves fitted together and held in place by two longitudinal lock bars with an H-shaped cross section, lock-bar pipe had a smoother interior and a greater carrying capacity than riveted pipe. As a result, it was used much more often than riveted pipe between 1915 and 1930.
Subsequently, developments in the relatively new field of automatic electric welding prompted new innovations. The massive demand for welding during World War II led to numerous improvements in technique, which helped to increase maximum pipe size during the 1950s. During the 1960s, experimentation began on the use of mortar lining and coating to increase the ring stiffness of steel pipe. In this way, steel was better able to maintain proper shape during installation.
With increased testing and field experience, engineers developed a design approach based on realistic performance limits, including stresses that exceed yield strength, excessive deformations, and leaks. They also developed advancements, such as cathodic protection and coatings, that combat corrosion.
Although people have been constructing buried pipelines for centuries, the field of pipeline design didn't begin until nearly 100 years ago, when Anson Marston became the first dean of engineering at Iowa State College.
Prompted by a desire to improve the condition of roads in Iowa, Marston led an effort to bury drainpipes along roadsides, eventually developing the first engineered design of buried drainpipe. In addition to devising an equation for soil load on pipes, he developed the first performance specification for rigid pipes, which required manufacturers to produce pipes that could withstand the "Marston load" in a three-edge bearing test.
Meanwhile, corrugated steel flexible pipe manufacturer Armco Co. sought to use its pipe as culverts.
To assist with these efforts, M.G. Spangler, another Iowa State College faculty member, studied the interaction of steel pipe and soil, revealing that flexible pipe deflects under soil load and develops horizontal support from soil on the sides of the pipe. Spangler and Reynold Watkins — a student with whom Spangler was collaborating and now an emeritus professor of engineering with Utah State University — developed what became known as the Modified Iowa Formula, which predicts the amount of deflection that will occur with flexible pipe under a certain vertical soil load. Published in 1958, the formula helped usher in widespread use of buried steel pipe.
Since then, however, engineers have frequently misapplied the formula, using it for a purpose other than what it was intended. Rather than simply using the equation to predict deflection, some pipe designers employed it to determine pipe thickness. In many cases, engineers using this approach have "over-designed" their pipes, making the wall thicker than necessary.
The manual was created, in part, to address the long-standing confusion regarding the proper use of the formula. Subsequent research by Watkins has found that soils surrounding a buried pipe provide most of the support needed by the pipe. For this reason, engineers using the formula should design the type of soil support they want for a buried pipe, rather than designing the pipe itself. In this way, engineers can conserve resources for their clients by avoiding the unnecessary expense associated with over-designed pipelines.
The manual also addresses such key concepts as pipe mechanics, soil mechanics, the interaction of pipes and soil, and design analysis. Special considerations, including parallel pipes in a common trench and trenches in poor soil, also are discussed. Appendices cover such topics as soil slip analysis, external fluid pressure, ring analysis, and impact factors in soil.
Over the past century, buried steel pipe has become a critical feature in countries around the world. Many pipelines installed decades ago remain in service; undoubtedly, many more will be installed, ensuring the continued use of this vital infrastructure component well into the future.
It's imperative that engineers and others involved in the design and construction of buried steel pipelines further their understanding of this technology. ASCE's manual of practice provides a foundation for ongoing efforts to further refine the design and construction of this key resource.
— Whidden (firstname.lastname@example.com) is a senior engineer IV in the Orlando office of consulting engineering firm PBS&J. As chair of the American Society of Civil Engineers' Buried Flexible (Steel) Pipe Load Stability Criteria and Design Task Committee, he was editor of the Manual of Practice 119, Buried Flexible Steel Pipe: Design and Structural Analysis.


text:
LoreleiPhoto says: "Good health & beauty through diet has always been important to me, and I wanted to share my enthusiasm by incorporating in a photoset my love of healing, whole, natural foods! Recently I learned about the importance of keeping your body properly pH balanced to prevent disease: The key is keeping your body more alkaline than acid. Fruits and vegetables are the most alkaline-forming foods we can eat, with lemons and watermelons being top on the list of the most alkalinizing foods. Luckily they're already two of my favorite snacks! Sprouts are also a superfood, and it's so easy to make your own - In less than 3 days I grew in a jar the sprouts you see here. Forget milk - it's gifts from the plant world that do a body the most good! See for yourself. ;) "
Only voters receive the private messages a model sends to her fans.
Voting is how you show love and appreciation to your favorite models & photographers.
Zivity pays them a cash royalty for each vote they receive.
I voted for this set because I'm a sucker for that gorgeous bespeckled face of yours and this set is full of shots of you in glasses.Dr_U
I love this set because of its simple sensuality and sense of fun. That, and you look gorgeous.babelmak1
lorelei eating watermelon...WOW! sexypetrostupid
I love your natural look! Those freckles along with your amazing body just makes me melt.....Amazing natural beauty...Thank you for sharing it!RainyAfternoon
I adore this set! So natural!MissChaos
Damn you are cute! Love your glasses and the freckles.VeeBee59
I love you so, gorgeous lady!Saint
i'm just so happy when you post a new set. yay!tmronin
Buy votes to support this artist on Zivity. Votes are $1 each, and when you vote on a set you like, 55% goes to the model and 30% to the photographer. You can vote in the next step after registering and purchasing votes.


text:
Note: This message is displayed if (1) your browser is not standards-compliant or (2) you have you disabled CSS. Read our Policies for more information.
Information about the current tax credits and incentives offered for a myriad of energy items from efficiency, both residential and commercial, to alternative fuels and the usage of renewable such as geothermal, wind and solar can be found on the following page:
As studies and energy trend projections become available, they will be included in this section. Current papers cover the topics of solar, wind, biomass, coal, ethanol and energy outlooks.
On the left hand navigational bar you will find a section called price watch. This provides the current fuel prices in Indiana and across the country as well as the current spot price for ethanol.
To stay apprised of current developments, please visit the Newsroom Page. Additionally if you would like to be notified of current developments, feel free to sign-up for email updates from OED, other Indiana partners and federal level groups. The Calendar of Events page lists a range of events that may be of interest.


text:
I thought I would share some of my other work with you here beginning with what started it all. Back in May (2008) my interest sparked in costuming and I wanted to make a vader costume. I decided to sculpt my own mask to try and cut costs and have a greater sense of pride in my work.
The first attempt was in drywall which I quit once I went back to school and had no place to work.
Here is the story of the new sculpt after around 15lbs of clay. This is my first time working with clay and or sculpting and I have learned a lot along the way.
Here are the most recent pictures today.
I'm doing the sculpt in sections to try and keep the sculpt from becoming lopsided and its been working fairly well. I believe I need to add a little more height to the forhead and add the top ring and then begin on the left side. I may have to order more clay soon as well so we'll see how long this will go.
I also have a Master Chief costume I'm working on and my Boba Fett but being at school I do my projects in the order I can in my dorm room. The Boba Fett is in the bondo and fiberglass stage and the Master Chief is on hold until the vader sculpt is done or I run out of clay.
Enjoy friends and Cheers!


text:
Subscribe to
KN Asus 23" Monitor Giveaway @Every 50 SUBscribers
Benefits include:
- Directly Support the Broadcaster
- Ad-Free*
- Subscriber Badges:
- 1 Subscriber Emoticons
- Chat During Subscriber-Only Mode
- Not Affected By Chat Slow Mode
*With limited exceptions.
$4.99 / Month
Sign in to Twitch
You need a (free) account to create your subscription. It should only take a moment and lets you access many other awesome Twitch features. At least we think they're awesome.


text: ESPN - OTL: The Disposable Superstar - E-ticket ENVER -- One of Chauncey Billups' ex-teammates -- and there are about a hundred of them -- has his nose pressed against a TV set. The Denver Nuggets have just called time, in the middle of a tight playoff game, and the ex-teammate can't help but notice Chauncey rounding up all the players, all the misfits and flakes, and poking a finger in their chests. If only these Nuggets understood the history behind this -- because there are pieces of Chauncey Billups strewn all over the league. He might have just rescued George Karl's job and Carmelo Anthony's résumé, but don't be fooled: Billups was once a mystery himself. His eyes are wiser now and his scars are healed, but no elite player in these NBA playoffs has been kicked to the curb as often as he has. None spent their first five seasons on six different teams. None has suited up for 10 different coaches. None has been forced to spend a 12th NBA season revalidating himself. In a league obsessed with LeBron, Kobe and D-Wade, Chauncey might actually be the one to emulate -- a sturdy playmaker who, mid-career, asked to be taught how to make plays. But now the journey ends where it began, a mile above sea level, with Chauncey teaching his own mercurial team how to pay attention to detail. It's why a zoo of an arena chants "MVP, MVP" in his direction. And it's why his former teammate from Minnesota, a friend he used to call "Unc," wishes he could jump through that TV screen, into that huddle … and tell the Nuggets the whole story. The whole story of "Smooth." For the rest click the link above.


text:
< 55€ < 77$
Couprie - Pineau Rosé
The blend Merlot, Cabernet, and Cognac provides a unique pale ruby-red colour. Barrel aging brings out heady, fruity flavours. There is a wonderful balance between sweetness and concentration. Best enjoyed at a temperature of 5-8° C.Learn MoreExcl. Tax: €13.33 Incl. Tax: €16.00


text:
|MK3 Interior Mods & Upgrades The place to chat about performance gauges, shifter knobs, custom interior paint, car seats or any interior upgrades for the 2012 Focus.
|Topic Review (Newest First)
|ST pedals should swap over - that would be a nice OEM upgrade.
Thoughts on some mods
I just got my 2013 Ford Focus Titanium in december and am loving it. I'm not a big car guy, but I would like to make it a bit more my own. So I had some thoughts about some mods I wanted to do and wanted to get some opinions on levels of difficultly and suggestion.
So my first idea was to change the pedal covers. For a cool looking car with ambient light it seems like the pedal covers are very bland. I figured I'd get a set with blue in them since my car is performance blue. Any thoughts on removing the existing covers and replacing them? I found in the how to section some tips, but it looked like the just attacked the new covers to the existing ones.
My second idea was a little bolder. I know there is a fix out there that costs 400 bucks that lets you bypass a setting and allows video to be run while the car is driving. I know it had other functions too. But I was thinking of wiring a switch to allow the bypass when needed. My though for this was to have my IPhone output it's video to the 8 inch screen for either video's for the kids or wife - or just be used with my navigation apps as a GPS. I figure this would probably be a bit hard since some paneling would need to be removed and such. The wiring I've done in the past so not to worried about that. Just need to know the wires to do this with.
Third idea was a bit out there for now. But I am a novice flight simulator builder as a hobby and I know some more advanced flight simulators have working HUDS on them. So I was thinking of seeing if I could create a HUD system for the car. Initially I would just want speed...but thinking if I hook up the system with a ODB2 connector I might be able to get more data then just speed (I have a ODB2 wifi setup and some cool Ipad/Iphone apps for it).
So thoughts on this? overall the car is amazing the way it is.
|Posting Rules
You may post new threads
You may post replies
You may not post attachments
You may not edit your posts
BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


text:
Wine Announcement
The Wine development release 1.7.24 is now available.
What's new in this release (see below for details):
- Beginning of some DirectWrite classes implementation.
- Initial wrapper dll for the packet capture library.
- Some crypto improvements.
- Various bug fixes.
- Login or register to post comments
- Printer-friendly version
- PDF version
More in Tux Machines
- Highlights
- Front Page
- Latest Headlines
- Archive
- Recent comments
- All-Time Popular Stories
- Hot Topics
- New Members


text: Hey guys, it has been a while since I have been here and I was wondering if anyone could refresh my memory on some armor I saw a while back. I can' remember the maker's whole name, but it was something like red dragon or something. I may be WAY off! But his armor was based on the WOF templates and went thru a couple revisions and was made from black ABS plastic. I swore the thread had something about budget armor in it, but now I can't find it. Anyone know what the heck I am talking about! lol!


text:
Elongase® Enzyme Mix is suitable for amplification of long as well as short DNA templates. Elongase® consists of a mixture of Taq and Pyrococcus species GB-D thermostable DNA polymerases. An optimized reaction buffer system is also provided which allows amplification of simple (e.g., plasmid and λ DNA) as well as complex genomic targets. Amplification of templates ≤12 kb can be readily obtained with Elongase®. For targets between 12 kb and 20 kb, reaction parameters need to be optimized. Amplification of targets longer than 20 kb is possible, but success is heavily dependent upon template and primer quality as well as careful optimization of reaction conditions.
Amplification using Taq DNA polymerase is generally limited to DNA templates less than 5 kb. This limitation is believed to be largely due to the inability of Taq DNA polymerase, which lacks proofreading (3' to 5' exonuclease) activity (1), to correct nucleotide misincorporations and continue primer elongation (2). Amplification systems which are able to amplify long targets have been described (3,4). In addition to the parent DNA polymerase, these systems contain a proofreading enzyme, which edits the nascent strand to allow subsequent polymerization by the parent enzyme. In this manner, Elongase® is able to amplify targets larger than 5 kb.
Component Part No. Amount
Elongase® Enzyme Mix Y02377 500 μL
5X Buffer A Y02371 5 × 1 mL
5X Buffer B Y02372 5 × 1 mL
Elongase® Enzyme Mix:
A Taq/Pyrococcus species GB-D DNA polymerase mixture in storage buffer [20 mM Tris-HCl (pH 8.0 at 25°C), 0.1 mM EDTA, 1 mM DTT, stabilizers, and 50% (v/v) glycerol].
Buffer System:
5X Buffer A [300 mM Tris-SO4, (pH 9.1 at 25°C), 90 mM (NH4)2SO4 and 5 mM MgSO4] and 5X Buffer B [300 mM Tris-SO4, (pH 9.1 at 25°C), 90 mM (NH4)2SO4 and 10 mM MgSO4] which can be differentially combined to optimize [Mg2+].
Intended Use
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Important Parameters to Consider:
- Design primers which will specifically anneal to the target of interest. For complex genomic targets, design primers with a high Tm (>60°C) so stringent primer annealing conditions can be used. It is convenient to design primers which will sufficiently anneal at 68°C so that a 68°C combined annealing and extension step (two-step cycling) can be employed during thermal cycling.
- For plasmid DNA, λ DNA, or other relatively abundant targets, use each primer at 400 nM. For more complex
amplification targets with a lower copy number such as genomic DNA, decrease the concentration of each primer to 200 nM. High primer concentrations, in some cases, initiate excessive competitive non-target amplification, especially when genomic DNA is used as target.
- The activity of Pyrococcus species GB-D DNA polymerase is inhibited in the presence of deoxyuracil-containing DNA. Therefore, use of dUMP-containing oligonucleotides with Elongase® for long amplification is not recommended.
Input template DNA:
- The quality of the template DNA is important. To amplify a large target, the input DNA must not be excessively sheared, nicked or otherwise damaged.
- If dilution of the input DNA is required, be sure to use sterile water as the diluent and not buffer. Carryover of
experimental sample buffer components along with the DNA may affect the amplification reaction with respect to [Mg2+], primer concentration and enzyme amount and, therefore, reactions may require re-optimization.
- Keep all components, reaction mixes and samples on ice. After preparation of the samples, transfer them to a pre-heated thermal cycler (94°C) and immediately start the amplification program.
- For most targets, 1 μL of enzyme mixture is sufficient. For smaller targets (less than 12 kb), 2 μL of enzyme mixture can be used to increase amplification yield. For longer targets, 2 μL of Elongase® is not recommended and may result in the appearance of non-specific products.
- Use ratios of Buffers A and B to determine optimal [Mg2+] for your amplification reactions. It is often useful to titrate Mg2+ concentrations between 1 and 2 mM (see ratio table). In most cases, Mg2+ concentrations between 1.6 and 2.0 mM are appropriate.
- In most circumstances, avoid using extensive pre-amplification denaturation which is longer or hotter than necessary since it may result in DNA template damage and reduced or no amplification. A temperature of 94°C for 30–60 s is recommended. Longer (3-min) pre-amplification denaturation times may be necessary for supercoiled DNA.
- Thin-walled amplification tubes, such as Perkin-Elmer GeneAmp® thin-walled reaction tubes, are recommended. Thickwalled tubes decrease amplification efficiency.
- It is recommended that Elongase® be used in 50-μL amplification reactions with the following thermal cyclers: The DNA Thermal Cycler, the GeneAmp® PCR System 9600 (Perkin-Elmer), and the PTC-100 Thermal Cycler (MJ Research, Inc.). For other thermal cyclers, reaction conditions may have to be optimized.
- Use aerosol-resistant tips (ART®) and DNA-free pipets for reaction set-up.
Prepare two separate mixes on ice, one containing template, primers and nucleotides (Mix 1) and another containing buffer and Elongase® (Mix 2). The two mixes can be subsequently combined in an amplification tube on ice or the reaction can be constructed using a "hot start" method.
- Add the following to corresponding microcentrifuge tubes placed on ice. Reaction cocktails can be made when multiple reactions are being constructed. It is important to pipette accurately, so use P2 and P10 pipets when dispensing small volumes. Be careful of any extra reagent which may be on the outside of the pipet tip.
Reaction set-up:
Volume per 50-μL reaction
Genomic DNA Target Other DNA Targets Final Concentration Mix 1 10 mM dNTP mix 1 μL 1 μL 200 μM each dNTP Forward primer, 10 μM 1 μL 2 μL 200 or 400 nM Reverse primer 10 μM 1 μL 2 μL 200 or 400 nM Template DNA x μL (≥100 ng) x μL (≥25 pg) --- Water to 20 μL to 20 μL --- Mix 2 5X Buffer A
10 μL total (Buffer A + Buffer B; see table)
60 mM Tris-SO4 (pH 9.1), 18 mM (NH4)2SO4, with MgSO4 between 1–2 mM 5X Buffer B Elongase® Enzyme Mix
1 μL (or 2 μL for targets < 12 kb)
to 30 μL
Buffer A and B ratio table:
Use the following amounts of Buffer A and B to achieve the desired Mg2+ concentration. The combined amount of Buffer A + B should equal exactly 10 μL:
Final [Mg2+] (mM)
1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2.0 A (μL) 10 9 8 7 6 5 4 3 2 1 0 B (μL) 0 1 2 3 4 5 6 7 8 9 19
- Pipette 20 μL of Mix 1 and 30 μL of Mix 2 into an amplification tube placed in ice. Make sure that all components are at the bottom of the amplification tube. Gently mix the tubes and overlay with two drops of the silicone oil provided. Silicone oil may be not be required for some thermal cyclers.
- Perform amplification. The following cycling conditions were established using a DNA Thermal Cycler 480 (Perkin-Elmer) and may have to be altered for other thermal cyclers. Remember that cycling conditions may have to be further optimized for difficult sequences and targets over 20 kb. Annealing and extension steps can be separate (three-step cycling), or they can be combined (two-step cycling) if the primers will sufficiently anneal at 68°C. For all low-copy targets, such as singlecopy genomic targets, use 35 cycles of thermal cycling.
A. For primers with a Tm below 68°C (three-step cycling):
1. Pre-amplification denaturation: 94°C for 30 s, 1 cycle
2. Thermal cycling: Denaturation; 94°C for 30 s
Annealing; 55–65°C for 30 s
Extension; 68°C for 45–60 s per kb of target ⇒30– &35 cycles
B. For primers with a Tm equal to, or greater than 68°C (two-step cycling):
1. Pre-amplification denaturation: 94°C for 30 s
2. Thermal cycling: Denaturation; 94°C for 30 s ⇒ 30–35 cycles
Annealing+Extension; 68°C for 45–60 s per kb of target
- Analyze amplification products. Load at least 20% (10 μL) of the amplification sample per gel lane on a 0.4–0.8% TAE agarose gel.
Product Qualification
The Certificate of Analysis provides detailed quality control information for each product. Certificates of Analysis are available here.
|Possible cause
|Possible solution
|No amplification product
|Template DNA concentration is not optimal
|Increase or decrease the concentration of template DNA. Use 50–200 ng of genomic DNA for single-copy targets. Use >25 pg for other targets. In some cases, large template amounts reduce amplification efficiency
|DNA is nicked or otherwise damaged
|Check quality by agarose gel analysis and replace DNA if necessary.
|Denaturation time and/or temperature is not optimal
|Increase or decrease time and/or temperature as necessary. Do not exceed 94°C for denaturation and try to keep denaturation times ≤30 s, except for supercoiled DNA.
|Extension time is too short.
|Maintain at least 45–60 s per kb of target length. For genomic targets, use 60 s per kb.
|Primer annealing temperature is too high.
|Decrease the annealing temperature as necessary.
|Cycle number is too low.
|Increase cycle number.
|Electrophoresis of PCR product yields a smear when stained with ethidium bromide.
|Enzyme amount is too high.
|Use 1 μL of Elongase® Enzyme Mix.
|Primer annealing temperature is too low.
|Increase the annealing temperature to increase the specificity of primer annealing.
|Mg2+ concentration is too high.l
|Titrate the Mg2+ concentration by mixing Buffers A and B (see ratio table)
|Multiple distinct bands are obtained by electrophoresis of PCR product
|Primer annealing temperature is too low.
|Increase annealing temperature to increase the specificity of primer annealing
|Amplification samples were not properly kept on ice
during reaction set up.
|Keep reactions on ice or use a "hot-start" method.
|Mg2+ concentration is not optimal.
|Titrate the Mg2+ concentration by mixing Buffers A and B
(see ratio table).
|Primer sequence(s) are
insufficiently specific.
|Re-examine primer sequences and redesign.
- Tindall, K.R. and Kunkel, T.A. (1988) Biochemistry 27, 6008.
- Innis, M.A., Myambo, K.B., Gelfand, D.H. and Brow, M.A.D. (1988) Proc. Natl. Acad. Sci. USA 85, 9436.
- Barnes, W.M. (1994) Proc. Natl. Acad. Sci. USA 91, 2216.
- Cheng, S., Fockler, C., Barnes, W.M. and Higuchi, R. (1994) Proc. Natl. Acad. Sci. USA 91, 5695.
MAN0000831 Rev. date: 7-Jun-2010


text:
Selling my stock class pump mag because I've decided it's time I need to start collecting firearms, so I'm trying to get some extra cash in for my first handgun. Since handguns aren't exactly cheap, I'm not really looking to deviate from my price too much, but I might if you're serious and have cash in hand. There's nothing wrong with it, it shoots beautifully, but I haven't shot it in a while, and I've never shot it over a chrono, so I don't know what it's shooting at or how consistent it is. As such, it will be sold as is, just in case. However, it is a mag, so if there is anything wrong, which I doubt there is, it's an extremely easy fix.
Marker: Stock Class Pump Mag
Condition: 8/10
Color: Stainless Steel and Black
Upgrades: Hitman Mod
Known Problems: None
Asking Price: $350 OBO
Shipping Options: USPS
Trades of interest: None
Location of item: Texas
What is Included: The pump, one of the four barrels, box of 10 round tubes, capless stock class pack, gun bag, stock
Edited by TK-421, 27 September 2012 - 04:01 PM.