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This tutorial touches on the creation of an MP3 with the use of the shape tools (rectangle, rounded rectangle) and many layer styles (gradient overlay, shadow, glow, bevel and emboss) to create the sleek, metallic effect of the player interface. An interesting thing that I have learnt is that there actually is such a font (free to download) called Guifx v2 Transports which allows users to type in the common symbols found in video and audio interfaces!
Read more from 'Creating Daily Objects From Scratch With Photoshop'.
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C. Dwayne Trammell, DDS
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Undergraduate & Medical SchoolUndergraduate: Baylor University
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Arrest Made in Soldiers' Deaths
A Fort Stewart sergeant has been arrested and charged with the deaths of the two soldiers found Sunday in a Hinesville townhome. Sgt. Shaquille D. Craig, is charged with two counts of murder for the deaths of Specialist Marquez Brown and Private Malika Jackson, who were found around 8:30 p.m. Sunday after an anonymous tip
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This word is an abbreviation for the often asked question, "Do I have stuff on my face?" After a meal, brushing your teeth, or plain out just being messy anybody would want to know whether or not their face is clean. By using this acronym, people can ask one another without feeling embarrassed and disrupting a whole conversation. After all, this simple word is much easier to pronounce and much quicker to say than that whole question.
After eating a baked good Caroline asks, "Hey, Nikki dihsomf?"
Nikki responds, "No Caroline, your good!"
Nikki responds, "No Caroline, your good!"
by ckz1208 June 08, 2014
The Urban Dictionary Mug
One side has the word, one side has the definition. Microwave and dishwasher safe. Lotsa space for your liquids.Buy the mug


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Mint and coral wedding theme
17 mistakes to avoid while planning your wedding
what are the easiest flowers to maintain | Tips-to-Keep-Hydrangea-Flowers-Blooming.jpg
We're revealing the roles and responsibilities of your mom and mother-in-law for your wedding, from your rehearsal dinner to your bridal shower!
Many different garden options, like shade, full sun, deck, etc. Worth checking out if you plan to plant a garden soon!
12 Things You Must Do The Morning Of Your Wedding
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Coral flower / Mint chiffon / wedding garter set / bridal garter/ lace garter / toss garter included / wedding garter
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Personalized photo calendars 2017 |vistaprint, Photo calendars make for unique and easy to create gifts! choose a design theme, add your photos & mark special dates for a personalized 2017 calendar.. Christmas 2016: 15 -chocolate advent calendars , There's so much chocolate around at christmas time that there's really no need for an extra piece each day behind the advent calendar door. we've found the best. The whisky advent calendar (2016 edition) whisky - master, It's the 2016 whisky advent calendar! look at it. regard it. what is surely the greatest advent calendar known to humankind is back for another christmas countdown.
Calendar 2017 (uk) - 16 free printable word templates, Template 1: calendar 2017 (uk) word colour, 1 page • year 2017 a4 page, landscape orientation • month colour ("rainbow calendar"). http://www.calendarpedia.co.uk/calendar-2017-word-templates.html Create calendar year - time date, This calendar creator generates calendar holidays countries. created, calendar shows dates year month.. http://www.timeanddate.com/calendar/generate.html Calendar 2017 uk bank holidays & excel/pdf/word templates, Calendar 2017 uk - yearly calendar 2017 united kingdom, bank holidays, week numbers free printable calendar templates excel, pdf & word. http://www.calendarpedia.co.uk/calendars/calendar-2017.html


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3564 Wesley Drive Gainesville, GA 30506
Investors take note leased home with tenants and income! 3 Bedrooms 2 baths sided Brick ,2 car carport on large level lots with fenced private back yard. Home is occupied and caution pets
- Listing Status Active
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- County Hall
- Year Built 1962
- Home Size 1175 sq.ft. (Source: not available)
- Sale Type Normal sale
- Elementary Sardis / Hall / Hall County, GA
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- High Chestatee / Hall / Hall County, GA
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Catholic Church Holy Angels
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July 1 2012, 10:12 PM
NYAFF 2012 Review: STARRY STARRY NIGHT, A Beautiful Portrait of Youth
Niels Matthijs
; Antwerp, Belgium
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With its New York premiere happening Tuesday, July 3rd as part of the 2012 New York Asian Film Festival, we now revisit Niels Matthijs review from earlier in the year.
Tom Lin's latest endeavor may be his definite ticket to international recognition, if
Starry Starry Night
makes it out of Taiwan (always somewhat of a gamble). It's a film with enough potential to appeal to a wide audience while at the same time maintaining its artistic integrity and firm traces of Lin's own personal voice. One thing is certain though, the result is one of the sweetest and most endearing films of 2011, warmly recommended to brighten up one of the upcoming winter evenings.
Lin has made some first-grade career choices these past few years. After brushing up his skills as first assistant director for a couple of quality productions (
Spider Lilies
The Wayward Cloud
Do Over
) and helming his very own first feature film (
Winds of September
), he now tackles the adaptation of a popular illustrated novel by Jimmy Liao. A smart move that landed him some extra budget to play around with, obviously well-spent.
Starry Starry Night
is the slightly fantastical tale of Mei, a 13-years old girl trying to deal with growing up, while still escaping to her own fantasy world when real-life problems prove to be too daunting to face. Mei finds a companion in Lee, an introverted young boy who just transferred from another school into Mei's class. When Mei's parents decide on a divorce, Mei runs off with Lee in search of a moment of untainted happiness and maybe even some romance.
The film is told completely from the perspective of Mei. Lin could've grabbed this opportunity to lose himself in overly detailed fantastical dreamworlds, instead he keeps the feel of
Starry Starry Night
much more down to earth, limiting most fantastical elements to background details or small touch-ups left and right. It gives the film a more mature atmosphere while still respecting the world of the main characters and their somewhat immature behavior.
Lin impresses royally with the film's visual style. Moody, warm and colorful, but also with a more serious and grim edge during the first, more reality-based half of the film. The CG is remarkably well accomplished, being both technically and aesthetically pleasing. One scene in particular, near the end of the film (the dream with the jigsaw puzzles) turns out to be a real stunner.
Starry Starry Night
is yet another film keeping the aesthetically honor of Taiwanese cinema high.
The soundtrack is a bit more on the safe side (as is usually the case with this kind of film).
Starry Starry Night
features a quality score with quite a few agreeable and atmopsheric tracks, but it does little more than support the scenes Lin had in mind. I prefer my soundtracks to be a bit more defining and out there, but I must say that the music here does add a layer of sugar-coated softness and glow that benefits the overall atmosphere.
As for the acting, Jiao Xu carries most of the film on her shoulders. Discovered in Stephen Chow's
she is now clearly expanding her reach into more complex and demanding roles. Lin gives her plenty of room to sparkle and Xu clearly grabbed that chance with both hands. Her performance is outstanding and gives a lot of extra shine to the Mei character. Eric Lin supports her pretty well, the rest of the secondary cast is solid but clearly not the main focus of
Starry Starry Night
If the poster art looks somewhat familiar, it may be because Woody Allen used the same Van Gogh painting to promote his latest film,
Midnight In Paris
(or because you've seen the Van Gogh painting before of course). The painting is more relevant for
Starry Starry Night
though, as it is actively featured during one of the key scenes and it is used to flesh out the key morale of the film. The whole art angle within Lin's film might feel a bit forced at first, but it does pay off rather well as the films progresses and it finds a perfect conclusion in the final scene.
While the epilogue is quite mushy and even sentimental, Lin shows his true mastership by making you believe this was the only ending possible. You can very well call it a feel-good Hollywood finale (and there is little to contradict that), but it's executed with so much class and warmth that any other conclusion would've felt wrong and out of place. The final shot is of stunning, unprecedented beauty and will leave you with a warm, contented feeling that carries on well beyond the last credits have disappeared from the screen.
Tom Lin paints one of the dearest, warmest and most charming films of the year.
Starry Starry Night
is a beautiful and imaginative trip into the mind of a 13-year old girl, exploring her world and dealing with her problems. The film has its fair share of memorable scenes and a truly magnificent ending that will keep you comfortably warm during these cold, rainy winter days. I'm already looking out for Lin's next film as I'm sure there's still a lot of unexplored potential left in him. For now though, I'll just recommend
Starry Starry Night
and hope that it will reach a big, loving audience that cherishes it accordingly.
As part of the 2012 New York Film Festival,
Starry Starry Night
plays Tuesday, July 3rd (3:30pm) at Walter Reade theater, Lincoln Center.
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Tom Lin
Jimmy Liao
Jiao Xu
Hui-Min Lin
Rene Liu
Harlem Yu
More about Starry Starry Night
North American Trailer For Tom Lin's STARRY STARRY NIGHT
STARRY STARRY NIGHT Review
HKAFF 2011: STARRY STARRY NIGHT Review
English-subbed Teaser And Official Trailer For Taiwan's STARRY STARRY NIGHT
Behold the beauty of Tom Lin's STARRY STARRY NIGHT
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The view from the peak of Prospect Mountain is absolutely amazing. You get a 360 view of the entire Lake George. It only costs $8 to entire the Prospect Moutain Memorial Highway which includes your car's drive to the parking lot and the viewmobile (bus) that takes you to the peak. If you plan on hiking, I'm not sure if you need to pay the $8. Don't cheap out, it's well worth the price.
There are a couple overviews on the way to the top so you can stop you car and take a couple pictures. But when you get to the parking lot, be sure to take the viewmobile to the summit. It's absolutely amazing. I recommend you pack yourself a nice lunch to have at the peak.
If you can, try to go the day after it rains. That's usually when you can get the best visibility. You will absolutely love it. But don't worry, even if the visibility is not 100%, you still get great views.
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Cell-free extracts isolated from colony biofilms of Actinobacillus pleuropneumoniae serotype 5 were found to inhibit biofilm formation by Staphylococcus aureus, S. epidermidis and Aggregatibacter actinomycetemcomitans, but not by A. pleuropneumoniae serotype 5 itself, in a 96-well microtiter plate assay. Physical and chemical analyses indicated that the antibiofilm activity in the extract was due to high-molecular-weight polysaccharide. Extracts isolated from a mutant strain deficient in the production of serotype 5 capsular polysaccharide did not exhibit antibiofilm activity. A plasmid harboring the serotype 5 capsule genes restored the antibiofilm activity in the mutant extract. Purified serotype 5 capsular polysaccharide also exhibited antibiofilm activity against S. aureus. A. pleuropneumoniae wild-type extracts did not inhibit S. aureus growth, but did inhibit S. aureus intercellular adhesion and binding of S. aureus cells to stainless steel surfaces. Furthermore, polystyrene surfaces coated with A. pleuropneumoniae wild-type extracts, but not with capsule-mutant extracts, resisted S. aureus biofilm formation. Our findings suggest that the A. pleuropneumoniae serotype 5 capsule inhibits cell-to-cell and cell-to-surface interactions of other bacteria. A. pleuropneumoniae serotype 5 capsular polysaccharide is one of a growing number of bacterial polysaccharides that exhibit broad-spectrum, nonbiocidal antibiofilm activity. Future studies on these antibiofilm polysaccharides may uncover novel functions for bacterial polysaccharides in nature, and may lead to the development of new classes of antibiofilm agents for industrial and clinical applications.
Citation: Karwacki MT, Kadouri DE, Bendaoud M, Izano EA, Sampathkumar V, Inzana TJ, et al. (2013) Antibiofilm Activity of Actinobacillus pleuropneumoniae Serotype 5 Capsular Polysaccharide. PLoS ONE 8(5): e63844. https://doi.org/10.1371/journal.pone.0063844
Editor: Christiane Forestier, Université d'Auvergne Clermont 1, France
Received: August 16, 2012; Accepted: April 9, 2013; Published: May 14, 2013
Copyright: © 2013 Karwacki et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This project was supported by grants from the Department of the Army (USAMRAA #W81XWH-12-2-0131), the National Institute of Allergy and Infectious Diseases (AI82329), and the Foundation of the University of Medicine and Dentistry of New Jersey. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Biofilm is the predominant mode of growth for bacteria in most natural, industrial and clinical environments [1]. Biofilms typically contain millions of tightly-packed cells encased in a polymeric matrix attached to a tissue or surface. The biofilm mode of growth protects bacteria from cell stressors such as desiccation, predators and antibiotics. Biofilms cause corrosion and biofouling of industrial equipment and chronic infections in clinical settings [2]. New methods for treating and preventing biofilm formation are being sought.
Many biofilm bacteria secrete molecules such as quorum-sensing signals [3], [4], surfactants [5], enzymes [6], [7], polysaccharides [8], and D-amino acids [9] that function to regulate biofilm architecture or mediate the release of cells from biofilms during the dispersal stage of the biofilm life-cycle. These compounds often exhibit broad spectrum biofilm-inhibiting or biofilm-dispersing activity when tested against biofilms cultured in vitro. Such compounds may represent a novel source of antibiofilm compounds for technological development [10].
Previous studies showed that colony biofilms may be a useful source of novel antibiofilm compounds [11]. Colony biofilms are lawns of bacterial cells cultured directly on an agar surface or on a semipermeable membrane that sits on an agar plate [12]. Colony biofilms have been shown to exhibit many properties characteristic of broth-cultured biofilms, including high cell density, extracellular matrix production, spatially dependent microbial growth, chemical gradients, and reduced susceptibility to antibiotics [13]-[18]. Cell-free extracts isolated from colony biofilms [11], or from biofilms cultured in a continuous-flow fermentor that produces a similarly high amount of biofilm biomass [19], have been shown to be enriched for soluble molecules produced within the biofilm matrix.
In the present study we screened a panel of 12 colony biofilm extracts isolated from various bacteria for their ability to inhibit Staphylococcus aureus biofilm formation in a 96-well microtiter plate assay. We found that colony biofilm extracts isolated from Actinobacillus pleuropneumoniae serotype 5 inhibited S. aureus biofilm formation without inhibiting S. aureus growth. Here we present results suggesting that the nonbiocidal antibiofilm activity in the A. pleuropneumoniae extract is due to high-molecular-weight serotype 5 capsular polysaccharide.
Bacterial Strains, Media and Growth Conditions
The bacterial strains used in this study are listed in Table 1. For solid media, Tryptic Soy agar was used for A. pleuropneumoniae, A. actinomycetemcomitans and H. influenzae, sheep blood agar (Catalog No. 221239; Becton, Dickinson and Co.) was used for staphylococci, and LB agar was used for all other bacteria. For broth cultures, Tryptic Soy broth supplemented with 6 g/L yeast extract and 8 g/L glucose was used for A. pleuropneumoniae, A. actinomycetemcomitans, H. influenzae and staphylococci, and LB broth was used for all others. A. pleuropneumoniae cultures were supplemented with 10 mg/L NAD. H. influenzae cultures were supplemented with 10 mg/L NAD and 10 mg/L hemin. Plasmid-harboring A. pleuropneumoniae strains were cultured in 80 mg/L spectinomycin. A. pleuropneumoniae and A. actinomycetemcomitans cultures were incubated in 10% CO2, whereas all other cultures were incubated in air. P. carotovorum cultures were incubated at 28°C, while all others were incubated at 37°C.
Preparation of Colony Biofilm Extracts
A 100-µl aliquot of an overnight broth culture (>108 CFU) was spread onto the surface of a 100-mm-diam agar plate using a sterile glass spreader. The plate was incubated for at least 48 h until a robust lawn of microbial growth (colony biofilm) developed. Thereafter, the cell paste was scraped from the surface of the plate using a plastic inoculating loop or plastic cell scraper, and the cells were transferred to a microcentrifuge tube containing 750 µl of saline (0.9% NaCl). The tubes were mixed by vortex agitation for 10 min, and the cells were pelleted by centrifugation. The supernatant was sterilized by passage through a 0.22-µm pore-size filter, and the resulting colony biofilm extract was stored at 4°C until use.
Screening Colony Biofilm Extracts for Antibiofilm Activity
S. aureus inocula were prepared from 18-h-old agar colonies as previously described [20]. A volume of 180 µl of inoculum (ca. 104–105 CFU/ml) was transferred to the well of a tissue-culture-treated polystyrene microtiter plate (Falcon no. 353047). A total of 20 µl of colony biofilm extract, or 20 µl of saline as a control, was mixed with the inoculum and the plate was incubated statically at 37°C. After 18 h, biofilms were rinsed with water and stained for 1 min with 200 µl of Gram's crystal violet. Wells were then rinsed with water and dried. The amount of crystal violet binding was quantitated by destaining the wells for 10 min with 200 µl of 33% acetic acid, and then measuring the absorbance of the crystal violet solution in a microplate spectrophotometer set at 595 nm.
Physical and Chemical Analyses of A. pleuropneumoniae Colony Biofilm Extract
Size-exclusion filtration was carried out using a Microcon centrifugal concentrator (Millipore) with a 100-kDa molecular weight cut-off filter. For enzymatic treatments, extracts were incubated for 1 h at 37°C with 100 mg/L DNase I, RNase A, porcine pancreatic lipase or proteinase K (Sigma-Aldrich) or 10 mg/L dispersin B (Kane Biotech). Controls consisted of mock-treated extracts, or enzymes alone with no extract. For sodium metaperiodate treatment, 0.1 vol of 100 mM sodium metaperiodate was added to the extract, and the extract was incubated at 37°C for 1 h. Controls consisted of mock-treated extract and sodium metaperiodate alone with no extract. Following all treatments, extracts and controls were incubated at 100°C for 10 min prior to testing in the S. aureus biofilm assay described above.
Purification of A. pleuropneumoniae Serotype 5 Capsular Polysaccharide
Capsular polysaccharide was purified from broth cultures of A. pleuropneumoniae strain J45 by Cetavlon precipitation of culture supernatant, extraction of the precipitate with NaCl and aqueous phenol, and Sepharose CL-4B gel filtration chromatography as previously described [21].
Cell Binding Assay
A single-cell suspension of S. aureus (ca. 106–107 CFU/ml) was prepared in fresh broth using a filtration protocol as previously described [22]. The cell suspension was supplemented with 10% A. pleuropneumoniae colony biofilm extract, or 10% saline as a control, and then aliquots of cells (0.5-ml each) were transferred to 1.5-ml polypropylene microcentrifuge tubes. Stainless steel rods (0.6-mm diam×13-mm length) were placed in the tubes, and the tubes were incubated at 37°C. After 30 or 60 min, rods were removed from the tubes, rinsed three times with saline, and transferred to 15-ml conical centrifuge tubes containing 1 ml of saline. The rods were sonicated on ice (2×30 sec) using an IKA Labortechnik sonicator set to 50% power and 50% duty cycle. CFUs in the sonicate were quantitated by dilution plating.
Intercellular Adhesion Assay
S. aureus was cultured in 17-mm ×100-mm glass tubes in 2 ml of broth. The broth was supplemented with 7% (by vol) A. pleuropneumoniae colony biofilm extract isolated from strain J45 or J45-100, or with 7% saline as a control. Bacteria were incubated with shaking (200 rpm). After 7 h, tubes were incubated statically for 10 min and then photographed.
Surface Coating Assay
A volume of 25 µl of A. pleuropneumoniae colony biofilm extract, or 25 µl of saline as a control, was transferred to the center of a well of a 24-well tissue-culture-treated polystyrene microtiter plate (Falcon no. 353047). The plate was incubated at 37°C for 30 min to allow complete evaporation of the liquid. The wells were then filled with 1 ml of broth containing 104 to 105 CFU/ml of S. aureus. After 18 h, wells were rinsed with water and stained with 1 ml of Gram's crystal violet. Stained biofilms were rinsed with water and dried, and the wells were photographed.
A. pleuropneumoniae inocula were prepared from 24-h-old agar colonies as previously described [20]. Bacterial inocula were diluted in fresh broth to 104–105 CFU/ml. Aliquots of diluted cells (250 µL each) were pipetted onto the surface of sterile glass slides, and the slides were placed inside a Petri dish. After incubation for 24 h, the slides were rinsed with water and stained with SYTO9 (Molecular Probes) for 20 min in the dark. Slides were then rinsed with water to remove excess stain. Biofilm bacteria were visualized at 10× magnification using a Nikon Eclipse 80i fluorescent microscope.
Statistics and Reproducibility of Results
All microtiter plate assays were performed in duplicate wells, which exhibited an average variation of <10%. All assays were performed 2–3 times with similarly significant differences in absorbance values. The significance of differences between means was measured using the Student's t-test. A P value of ≤0.05 was considered significant.
A. pleuropneumoniae Colony Biofilm Extract Exhibits Antibiofilm Activity
We isolated colony biofilm extracts from 12 different bacteria and tested the extracts for their ability to inhibit biofilm formation by S. aureus in a 96-well microtiter plate assay (Fig. 1). The bacteria that were tested comprised a convenience sample of 11 Proteobacteria and Lactococcus lactis (Table 1). Extracts were tested at a concentration of 10% by vol. Under these conditions, five extracts significantly inhibited S. aureus biofilm formation, while seven extracts had no significant effect on biofilm formation. Inhibition of S. aureus biofilm formation by P. aeruginosa extract was partially due to growth inhibition (data not shown). We selected A. pleuropneumoniae IA5 colony biofilm extract for further analysis because it exhibited a high level of biofilm inhibition, but it did not inhibit S. aureus growth or contain proteases or DNases that are known to inhibit S. aureus biofilm formation (data not shown). A. pleuropneumoniae IA5 colony biofilm extract inhibited biofilm formation by S. aureus, S. epidermidis, and the Gram-negative Aggregatibacter actinomycetemcomitans (Fig. 2). A. pleuropneumoniae colony biofilm extract did not inhibit the growth of S. aureus, S. epidermidis or A. actinomycetemcomitans (data not shown).
S. aureus was cultured in the presence 10% extract isolated from the bacteria indicated along the bottom. Control cultures were incubated with 10% saline. After 18 h, biofilms were rinsed and stained with crystal violet. Values show the average amount of crystal violet binding for duplicate wells and error bars indicate range. Asterisks indicate absorbance values significantly different from saline control (P<0.05).
Inocula were supplemented with 10% A. pleuropneumoniae IA5 extract, or 10% saline as a control. Bacteria were cultured in 96-well microtiter plates. After 18 h, biofilms were rinsed, stained with crystal violet, and photographed. Duplicate wells are shown.
The Antibiofilm Activity in A. pleuropneumoniae Colony Biofilm Extract is due to Capsular Polysaccharide
Physical analysis of the A. pleuropneumoniae IA5 colony biofilm extract indicated that the antibiofilm activity in the extract was >100 kDa in mass (Fig. 3A) and heat stable (Fig. 3B). Treatment of the extract with proteinase K, lipase, DNase or RNase had no effect on its antibiofilm activity (Fig. 3C). In contrast, treatment of the extract with the carbohydrate-active agent sodium metaperiodate significantly reduced its antibiofilm activity (Fig. 3C). These data suggest that the antibiofilm activity in the A. pleuropneumoniae colony biofilm extract was due to high-molecular-weight polysaccharide.
(A) Biofilm formation by S. aureus in the presence of 10% saline (control), crude extract, or the filtrate and retenate of crude extract passed through a 100-kDa pore-size filter. Duplicate wells are shown. (B) Biofilm formation by S. aureus in the presence of 10% saline, crude extract, or crude extract that was incubated at 100°C for 15 min. Duplicate wells are shown. (C) Biofilm-inhibiting activity of IA5 extract treated with proteinase K, lipase, DNase, RNase or sodium metaperiodate. Biofilm inhibition was measured against S. aureus as in panels A and B. Percent activity was calculated as the ratio of the reduction in crystal violet absorbance exhibited by the treated extract to the reduction in absorbance exhibited by the mock-treated extract (ΔA595(treated extract)/ΔA595(mock-treated extract) × 100). The graph show mean and range percent activity values from 2-3 experiments. Asterisk indicates a significant reduction in activity (P<0.05).
To determine whether the A. pleuropneumoniae serotype 5 capsule contributes to the antibiofilm activity of the colony biofilm extract, we isolated extracts from wild-type serotype 5 strain J45, and from the isogenic serotype 5 capsule-mutant strain J45-100. Like extracts isolated from strain IA5, extracts isolated from strain J45 inhibited S. aureus biofilm formation (Fig. 4A). In contrast, extracts isolated from capsule-mutant strain J45-100 did not inhibit S. aureus biofilm formation (Fig. 4A). Extracts isolated from capsule-mutant strain J45-100 transformed with plasmid pJMLCPS5, which restores capsule production, exhibited significantly greater antibiofilm activity than extracts isolated from uncomplemented J45-100 (Fig. 4B). This biofilm inhibition was not due to growth inhibition caused by antibiotic carryover from the genetically-complemented A. pleuropneumoniae culture (data not shown). In addition, purified serotype 5 capsular polysaccharide inhibited S. aureus biofilm formation in a dose-dependent manner (Fig. 4C). These findings confirm that the A. pleuropneumoniae serotype 5 capsule exhibits antibiofilm activity against S. aureus.
(A) S. aureus biofilm formation in the presence of colony biofilm extracts isolated from wild-type strains IA5 and J45, and isogenic J45 capsule-mutant J45-100. Duplicate wells are shown. *, significantly different from J45 control extract (P<0.05). (B) Quantitation of S. aureus biofilm formation in the presence of extracts isolated from wild-type J45, capsule mutant J45-100, and genetically-complemented J45-100 capsule-mutant. (C) Quantitation of S. aureus biofilm formation in the presence of purified serotype 5 capsular polysaccharide. Values in panels B and C show averages for duplicate wells and error bars indicate range.
A. pleuropneumoniae Colony Biofilm Extract Inhibits S. aureus Cell-to-cell and Cell-to-Surface Interactions
S. aureus cells cultured in broth with shaking aggregated and settled to the bottom of the tube, resulting in a visible clearing of the broth (Fig. 5A, left panel). When A. pleuropneumoniae J45 extract was present in the culture, the S. aureus cells exhibited less settling (Fig. 5A, middle panel). S. aureus cells cultured in the presence of extract isolated from serotype 5 capsule-mutant strain J45-100 exhibited the same amount of settling as control cultures (Fig. 5A, right panel). These data suggest that A. pleuropneumoniae serotype 5 capsule inhibits S. aureus intercellular adhesion.
(A) Intercellular adhesion of S. aureus planktonic cells cultured in 10% saline (control), wild-type J45 extract, or capsule-mutant J45-100 extract. Duplicate tubes are shown. (B) Attachment of S. aureus planktonic cells to stainless steel rods in the presence of 10% A. pleuropneumoniae J45 colony biofilm extract. Values show average for duplicate rods. (C) Biofilm formation by S. aureus in polystyrene microtiter plate wells coated with saline, J45 extract, or J45-100 extract. Duplicate wells are shown.
We also measured binding of S. aureus planktonic cells to stainless steel rods in the presence and absence of A. pleuropneumoniae J45 colony biofilm extract. As shown in Fig. 5B, the A. pleuropneumoniae extract significantly inhibited S. aureus cell binding after both 30 and 60 min.
We also tested whether A. pleuropneumoniae J45 colony biofilm extract could modify the surface properties of an abiotic substrate. To do this, we used evaporation coating to deposit the extract onto the surface of polystyrene wells, and then tested the ability of the coated surfaces to resist biofilm formation by S. aureus. When A. pleuropneumoniae extract was applied to the polystyrene surfaces, the coated surfaces efficiently repelled S. aureus biofilm formation in the area where the extract was deposited (Fig. 5C). Surfaces coated with extract isolated from the serotype 5 capsule-mutant strain did not resist S. aureus biofilm formation (Fig. 5C).
A. pleuropneumoniae Colony Biofilm Extract does not Inhibit A. pleuropneumoniae Biofilm Formation
Extracts isolated from A. pleuropneumoniae wild-type strain J45 and capsule-mutant strain J45-100 were tested for their ability to inhibit biofilm formation by A. pleuropneumoniae J45 and J45-100. There was no significant difference between the amount of biofilm formed by strains J45 and J45-100 when quantitated by spectrophotometry (Fig. 6A). Neither the J45 nor the J45-100 extract significantly inhibited biofilm formation by either strain. Dispersin B significantly inhibited biofilm formation by both strains, indicating that strain J45, like A. pleuropneumoniae serotype 5 strain IA5 [23], produces PNAG-dependent biofilms. Microscopic analysis revealed that biofilms produced by the capsule mutant strain J45-100 appeared much denser than biofilms produced by wild-type strain J45 (Fig. 6B).
(A) Quantitation of biofilm formation in 96-well polystyrene microtiter plates. Biofilms were grown in the presence of 10% saline (control), J45 colony biofilm extract, J45-100 colony biofilm extract, or 10 mg/L dispersin B. Biofilms were stained with crystal violet. Values show mean absorbance values and standard deviation from four independent experiment. *, significantly different from saline control (P<0.05). (B) Micrographs of 24-h-old biofilms cultured on glass slides. Cells were stained with SYTO9. Measure bar = 20 µm.
The Gram-negative bacterium A. pleuropneumoniae is the causative agent of swine pleuropneumonia, a severe and contagious respiratory disease that affects pigs worldwide [24]. A. pleuropneumoniae strains are divided into 15 serotypes based on the structures of their capsular polysaccharide (CPS) [25]. Serotype 5 is a prevalent A. pleuropneumoniae serotype in the U.S., Canada, Brazil, Chile, Korea and Taiwan [26]. A. pleuropneumoniae serotype 5 CPS consists of a linear polymer with the structure →6)-α-D-GlcpNAc-(1→5)-β-D-dOclAp-(2→ [27]. A subset of serotype 5 strains (designated serotype 5b) contain an additional β-D-Glcp reside covalently joined to the β-D-dOclAp residue in (1→4) linkage [26]. Mutant strains lacking serotype 5 CPS have been shown to exhibit decreased serum resistance in vitro and decreased virulence in pigs [28], [29].
Our findings demonstrate that A. pleuropneumoniae serotype 5 CPS exhibits nonbiocidal antibiofilm activity against other Gram-negative and Gram-positive bacteria. Several other bacteria produce nonbiocidal antibiofilm polysaccharides including Kingella kingae [11], Escherichia coli [8], [19], Bacillus licheniformis [30], Lactobacillus acidophilus [31], Streptococcus phocae [32] and Vibrio sp. [33]. The biological functions of these antibiofilm polysaccharides are not known, but they may include water channel formation or biofilm dispersal [34]. The fact that the A. pleuropneumoniae CPS mutant strain exhibited increased biofilm formation compared to the wild-type strain (Fig. 6B) suggests that the serotype 5 capsule may function in water channel formation or biofilm dispersal. Previous studies showed that A. pleuropneumoniae produces dispersin B, which may also function in maintaining biofilm architecture or in biofilm dispersal [23].
We found that A. pleuropneumoniae serotype 5 CPS inhibited biofilm formation by S. aureus, S. epidermidis and A. actinomycetemcomitans, but not by A. pleuropneumoniae serotype 5 itself. Biofilm formation by all four of these species is dependent on the production of poly-N-acetylglucosamine (PNAG) matrix polysaccharide [20], [35]. Although PNAG is the major biofilm matrix adhesin in both A. pleuropneumoniae and S. epidermidis biofilms, A. pleuropneumoniae serotype 5 CPS inhibited only S. epidermidis biofilm formation and not A. pleuropneumoniae serotype 5 biofilm formation. In addition, colony biofilm extracts isolated from a PNAG-deficient strain of A. pleuropneumoniae serotype 5 exhibited the same antibiofilm activity as extracts isolated from a wild-type strain (unpublished results). These observations suggest that the antibiofilm activity of serotype 5 CPS is independent of the intercellular adhesion activity of PNAG.
Our screen of colony biofilm extracts isolated from 12 phylogenetically diverse bacteria identified five extracts that exhibited antibiofilm activity against S. aureus. In a similar screen, Rendueles et al. [19] found that 20% of cell-free biofilm extracts isolated from 122 natural E. coli isolates exhibited antibiofilm activity against a panel of seven biofilm-forming Gram-positive and Gram-negative bacteria. These findings suggest that bacterial biofilms constitute untapped sources of natural bioactive molecules antagonizing adhesion or biofilm formation of other bacteria.
Author Contributions
Conceived and designed the experiments: DEK TJI JBK. Performed the experiments: MTK MB EAI VS TJI. Analyzed the data: MTK DEK TJI JBK. Wrote the paper: MTK DEK TJI JBK.
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This Is Why Latin America is Huge Among Retirees in 2017, Part 1
Six of the Top 10 places to retire around the world in 2017 are in Latin America, according to International
Six of the Top 10 places to retire around the world in 2017 are in Latin America, according to International
Hello again! Here´s Part 3 of your series, looking at the boaty and touristy experiences you can enjoy on Lake
So, last time we looked at where Lake Titicaca bloomin´ well is, and some of the ancient myths and ruins
There are some areas of the world almost no one has sailed around, and one of those is Lake Titicaca,
Naturally, there´s nothing like home. But if you do fancy an adventure, wouldn´t it be cool to include some of
The City Cochabamba is known as the city of eternal spring because of its sumptuous climate. One of Bolivia's
This bearded chap certainly gets around. I mean, he even gets kissed by some alpacas. Pecked by a penguin and
Got your helmet? Ready to see some exquisitely beautiful mountains, long stretches of open road with a loved one, and
Professional footage can make you feel like you really are in a place. Wouldn't it be nice to escape your
500 Days Holiday Anyone? I wonder if long trips hurt riders' behinds! This guy is so dedicated. Watch him go


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ALL >> Business >> View Article
The Options For Uncomplicated Best Shampoo Methods
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Many alkaline mostly based shampoos actually make an environment that could injury your hair as well as your skin. Your hair has a pH equilibrium of four. 5 to 5.5. If you are putting hair shampoo and conditioners and various other hair options on your hair that are not in your pH facets, it could hurt your hair. Make persuaded to examine your elements and labels when you obtain your shampoos and conditioners. Sources for hair resulting has been connecteded to hair shampoos that are planned to make a great deal even more body. Some hair items might potentially essentially offer a lot more physique but at the worth of damaging your hair. THE Widespread Affects OF SOME Improper SHAMPOOS! Shampoos with the incorrect alkaline security could give the aesthetic charm that your hair has included figure and quantity but could result in hair to be weak and ultimately leave. If a shampoo is specifically greater in alkaline it can likewise result in a modify in hair color that is also harmful. In conformity to hair expert Jonathan E. Previously mentioned all points, use hair shampoo and conditioners with all-healthy elements. You may feel you're getting an efficient offer with $.99 hair shampoo however it truly is not a good discount for wholesome hair! Second of all, make good it is eco-friendly which will certainly not bring in filth and oils to congest your pores. Finally, protect against animal elements. No matter just what is used on the pets, you additionally get a dosage! There are fairly a few hair shampoos and various other hair treatment items that are provided at well being meals electrical outlets that do not consist of things like hazardous materials to your hair. When starting up my hair thinning device, holding my hair complimentary of charge of hair items with chemical components was 1 of many methods that assisted me flip my loss of hair pictures into hair repair! Viewing that then, I have actually produced a bald place in back once more to virtually disappear or even significantly recover a receding hairline. It has been the distinction involving becoming absolutely bald and really creating brand-new hair as well as my self-self self-confidence! HAIR Decrease Factors Associated TO HAIR Care Product! You could think that everything on your grocery rack is safe. That was my thinking about when I was a more youthful kid, yet you might still feel this is appropriate. I want you acknowledge that not only do drugs acquire recalled. Did you understand that most hair treatment item or solution components pass exceptionally limited screening? However, the majority of us are prepared to set anything on our just click the following internet page hair! We never ever end and believe that our hair loss motives could be connected to our hair products. If you truly wish to stop hair thinning, commence with using hair treatment things not having chemical materials! Although searching for an organic method to stop my severe hair decrease, I discovered sufficient shocking info concerning firms that do not wait to market products that hurt, disable and damage, simply for a buck! It actually is not merely all the drug corporations that have actually facet results! Underneath is a checklist (however not all comprehensive) of hair procedure options that I stay free from putting in my hair.
The very best shampoo for thinning hair in females has impressed everybody, from users to hair experts. At last, they have actually found a product that offers preferred outcomes on hair. Shampoos are mostly anticipated to clean hair, removing all the filth and grime, excess oil, and various other debris from fibers and scalp. Frequently, individuals are pleased with an item that offers great deals of lather, as it is thought that additional lather means a far better clean.
This is where shampoo business make the many. They infuse their shampoos with sulfates, which are extreme cleaning agents popular for creating great deals of foam. Contribute to this a touch of scent and your hair cleaning experience comes to be heavenly to the detects. You like your fragrant shower!
Exactly what you don't recognize and just what these firms have not informed you is sulfates injury hair. Extended select can make your scalp flaky and completely dry, or cranky and scratchy. Sulfates remove off all oil from hair and scalp and this includes necessary oils. Thats why your hair really feels rough and dry after washing with a sudsy shampoo.
Research has actually developed that sulfates are skin and scalp irritants. Scientists are even suspicious that they may be carcinogenic. Study in this area is in progress.
Thanks after reading that article. To learn more regarding the study, kindly visit our blog. We are certain that you will definitely find out tons of various other very useful data.
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13. What Are The Video Conferencing Perks For The Retail Sector?
Author: Doyel
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Discussion in 'Social Life, Food & Drink, Travel' started by johnapril, Jun 23, 2005.
Everyone look to the left. Satan.
WTF, man. Is that the most intelligent analysis/criticism you can muster to describe someone with whom you disagree?
Or, if you literally believe it, can you explain how/why you think he's actually evil? That's just silly.
Lindsay, it's John April. He is high on... life, perhaps. Take it as a joke.
You're right, LA. It was just so funny, I lost my mind for a moment.
It might be funny once, but does he have to post it in every goddamn thread in every forum? And shouldn't this thread be moved to News & Current Events?
What, you didn't find it to be infinitely more effective? He's just staying on message.
I'm guessing it was an attempt to have your name on all forums at once... I think faustianbargain (?) managed this before, I'd assume much easier late night.
i've done it twice. someone else has done it once, too...maybe RJMan? i can't recall.
anyway one time at least was during the day. i guess the other time was at night.
not my proudest moments, but at least it was something.
Separate names with a comma.


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SEE ALSO: Lee Jung Hyun says she was aware of co-star Song Joong Ki's relationship prior to public announcement
On September 17, Lee Kuk Ju referred to something she said on the January 1st episode of MBC's 'I Live Alone'. She had previously written, "I want to start dating on September 17. If I start dating that day, then Christmas would be our 100th day together." At the time, the comedian said her ideal type was "a man who could love me at my worst state."
Today, she posted, "It's today. I'm wondering about my future for the first time in a while. What are you all worrying about?"


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Apartments for Rent in Greenbrae, CA | Aqua Terra - Home
Come to a home you deserve located in Greenbrae, CA. Aqua Terra has everything you need . Call (866) 883-7155 today!
CA Apartments Great Greenbrae, CA, Apartments for rent in CA, (866) 883-7155 Apartments CA
Last Update: MEZ/CET 26.07.2017, 00:06:57 Uhr


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Opening tomorrow on VOD from IFC Films is Dennis Gansel's We Are the Night, "a sexy and suspenseful thriller about a young woman initiated into a trio of beautiful female vampires." It will be released theatrically in New York City in rep on May 27 at the prestigious ReRun arthouse theater alongside VOD.
David Harley wasn't a big fan: " 'We Are The Night' is as conventional as possible, with Gansel and Jan Berger's screenplay eschewing ingenuity for premises and scenes that were more easily stolen from other films. The concoction they managed to throw together – which was reportedly rewritten because it resembled 'Twilight' a little too much – is bland and forgettable, proving that just because the ideas seen at play in vampire films are becoming as immortal as the creatures themselves, it doesn't mean they should be."
Click the title above for the entire review.
"Dennis Gansel, director of the critically acclaimed THE WAVE, explores a seething dark side of Berlin in the WE ARE THE NIGHT. Following a sect of seductive female vampires that hide out in the city's alternative clubs, enjoying the luxury and pleasures their attained immortality provides them and wreaking horror on a string of unsuspecting victims, the film is a brilliantly stylized vision of the classic vampire myth — a sensual, charismatic thrill ride fascinated with all aspects of the night."
this week in horror
This Week in Horror - June 26, 2017 - The Evil Within 2, Jason...
The Evil Within 2 was shown at E3, Victor Miller is trying to get the rights back to Jason Voorhees, and Saw: Legacy has an official title! It's This Week in Horror with Whitney Moore!Posted by Bloody Disgusting on Monday, June 26, 2017
Trending Now
Here's the "American Horror Story" Season 7 Title Reveal! #SDCC
[Trailer] Guillermo del Toro's 'The Shape of Water' Takes the Creature Out of the Lagoon
Freddy Krueger Appears in Epic 'Ready Player One' Trailer! #SDCC
Preview Target's Halloween 2017 Collection!
The New 'IT' Kids Pick Who They Want to Play Them as Adults


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Explore Shabby Cottage, Cottage Chic and more!
Explore related topics
shabby cottage with homer laughlin pink rose dishes -- idea for my hutch
Shabby chic hutch in pink & white
Love it, but I don't think I'm allowed large pieces of pink furniture! :) pink & white china cabinet, shabby chic
This is sort of how I always envisioned Madam Puddifoot's Tea Shop in Hogsmeade when Harry and Cho go there on their date, only even more pink n fussy
I would like this in my home
Shelving unit for decorative china (Grandma seems to like putting plates on the wall)
Sweet old fashioned kitchen
Shabby chic
Shabby pink roses china- I must have these!
Love it! Use a pretty frame to finish off a built in shelf unit. Great idea for a medicine cabinet, or spice rack


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Opened only a year ago. Fabulous hotel. Unlike most Intercontinental hotels I know (including those in Tokyo), which are all solid 4 star hotels, the Osaka Intercontinental is a step up, 5 star all the way, competing head to head with the St Regis or Peninsula or Four Seasons type hotel. The room was very grand (to be fair we were upgraded, I am IHG Platinum), with spectacular views. The service of the staff in both the hotel itself, and the restaurants, was impeccable (our request to be seated between meals, at 10.30am, caused them inconvenience but they bent over backwards to accommodate us).
If you are arriving for the first time via Umeda or Osaka stations: even though it is close, don't try to get there underground. Get to street level. Find the red Hep Navis ferris wheel, the Yodabashi store, or the Intercontinental building itself (it has the name at the top) to get your directional bearings. The stations underground are rabbit warrens and the Intercontinental is not easily accessed via the mall level (although you will be able to figure the route when departing the hotel, as opposed to arriving).
- Reservation Options:
- TripAdvisor is proud to partner with IHG, Booking.com, Expedia, Hotels.com, Ctrip TA, Priceline, Odigeo, Rakuten, Ebookers, 5viajes2012 S.L., TripOnline SA, HotelQuickly, Cancelon and getaroom.com so you can book your Intercontinental Hotel Osaka reservations with confidence. We help millions of travellers each month to find the perfect hotel for both holiday and business trips, always with the best discounts and special offers.


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oil burning faster than i can add it to my 2002 monte carlo ss
1 Answer
Your engine is worn out - sorry. Does it smoke a lot or leak the oil? If it is leaks you may want to fix the leaks but massive oil burning indicates a worn out engine or other major problem. The solution is a rebuild or a new engine.
Your Answer
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Starbucks Unveils $7 Wake-Up Slap
SEATTLE—Touting it as an invigorating way to jumpstart your morning, Starbucks on Friday unveiled its new $7 wake-up slap.
Once heralded as a shrewd innovator, embattled CEO Ken Lewis is now leaving Bank Of America. Here are some key missteps from the past 18 months that have cast a pall over his tenure:


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Martes (2)
The best exercises to give your bust line a lift and make your breasts appear bigger and perkier, the natural way!
semana uno: lunes 26 dic. semana dos: lunes 2 ene. semana tres: lunes 9 ene. semana cuatro: lunes 16 fin de ejercicios domingo 22 ene.
30 day plank challenge for beginners before and after results - Try this 30 day plank exercise for beginners to help you get a flat belly and smaller waist.
Rutina para tener cintura marcada y abdomen plano. Flat abs and slim waist.
Ejercicio para caderas
Ejercicios - Tonificación de piernas!
Beach Icons by Evan Marks, via Behance
So you have been doing your butt workouts for weeks or maybe even months and yet you're still not getting good results. It seem as if no matter how hard you try


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The atmosphere and service are excellent, the rooms were clean and the beds were VERY comfortable. The owners are friendly and helpful. I particularly like the conservatory, it was a great place to chill out and the owners had thought of everything from tea & coffee to paracetamol & after sun, thanks guys. I would highly recommend anyone stay here, it is probably the best place I have stayed and it felt like leaving family when it was time to go home.
- Official Description (provided by the hotel):
- The Griffin is a Georgian Townhouse in the heart of Brighton. The Griffin Guest House is a few yards from St. James Street, and also just off the seafront, you can be on the Pier in less than two minutes. The Griffin has undergone a thorough renovation in recent times and offers clean modern accommodation, lodged in a charming building dating from 1760, the hotel has nine rooms, each with its own en suite Whirlpool bath or shower ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Priceline, Agoda and Odigeo so you can book your The Griffin Guest House reservations with confidence. We help millions of travellers each month to find the perfect hotel for both holiday and business trips, always with the best discounts and special offers.
- Also Known As:
- The Griffin Guest House Hotel Brighton


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Visitor Information
LondonTown.com - Chandni Location Map
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Discover what's on in London 2017 with our comprehensive guide to the best events. From theatre to art and music to sport, London 2017 has something for everyone - so read on to find out what London town has to offer.
Hotels near Chandni
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London > Hotels > Mid-Range Chain Hotel from £66 34% off
10A Chestnut Plaza, E20 | 31 minutes from Chandni
Feel at home as soon as you enter the contemporary lobby of the Holiday Inn London Stratford City. This new generation Holiday Inn is located in ... More
Alternative Vegetarian restaurants nearest Chandni
London > Restaurants > Vegetarian restaurants
21 Old Ford Road, Tower Hamlets, London E2 | 3.6 miles from Chandni
Vegetarian cuisine. ... More
London > Restaurants > Vegetarian restaurants
Elys Yard, Old Truman Brewery, Brick Lane, Spitalfields, London E1 | 4.6 miles from Chandni
The Routemaster bus occupies a special place in the hearts of Londoners. And if the old adage - 'the best way to a man's heart is ... More
London > Restaurants > Vegetarian restaurants
3 Crispin Place, Spitalfields, Spitalfields, London E1 | 4.8 miles from Chandni
When Leon first threw open its doors in Carnaby Street a few years ago workers of London rejoiced - well, the glamorous girls who work in ... More
Dining near Chandni
London > Restaurants > Indian/South Asian restaurants
875 High Road, Leytonstone, E11 | 9 minutes from Chandni
Enjoying a glowing reputation and wide acclaim from the press, Star of India has served East London with sumptuous flavours for over 3 decades. Imaginative combinations ... More
London > Restaurants > Indian/South Asian restaurants
146 High Road Leytonstone, E15 | 17 minutes from Chandni
Indian/South Asian Cuisine... More
London > Restaurants > French restaurants
17 High Street, Wanstead, E11 | 24 minutes from Chandni
Recipient of a Bib Gourmand award, Provender is an informal all-day bistro that serves well-sourced French food and wine. ... More
Entertainment near Chandni
London > Entertainment > Cinemas
Gerry Raffles Square, Salway Road, E15 | 30 minutes from Chandni
Fitted out to luxury standards, Stratford Picturehouse is one of 20 independent cinemas operated by Picturehouse which also owns Clapham Picturehouse, the Gate Picturehouse, Greenwich Picturehouse, ... More
London > Entertainment > Theatres
Gerry Raffles Square, E15 | 30 minutes from Chandni
Theatre Royal Stratford East opened in 1884 and became the home of the Theatre Workshop Company in 1954. The first performance ever held here was 'Richelieu' ... More
London > Entertainment > Theatres
Theatre Square, E15 | 30 minutes from Chandni
Plans to construct The Red Crate, a new theatre opposite Theatre Royal Stratford East, are on hold. A striking 220-seat eco-theatre similar to ... More
Sights and Attractions near Chandni
London > Attractions > Attractions for Kids
Skeltons Lane Recreation Ground, 135 Skeltons Lane, E10 | 20 minutes from Chandni
Part of Capel Manor College, Brooks Farm, is a place to study and it's also a wonderful visitor's attraction with goats, sheep and pigs ... More
London > Attractions > Other Attractions
E20 | 25 minutes from Chandni
The former Athletes' Village for the 2012 London Olympics, East Village is London's first Olympic legacy village providing homes, shops, restaurants, cafes and bars on the ... More
London > Attractions > Historic Buildings
29 Broadway, E15 | 31 minutes from Chandni
Once described as the 'finest building in Essex', the Grade II listed Old Town Hall in Stratford has been restored to full Victorian splendour with particular ... More
Shopping near Chandni
London > Shopping > Art and Design
Mother Studios, Queens Yard, White Post Lane, E9 | 2.1 miles from Chandni
Elevator Gallery is a contemporary artist-run space in Hackney Wick, London and is housed in the attic of a former chocolate factory, above Mother Studios. Curators ... More
London > Shopping > Food and Drink Shops
Unit 7.2, 3-4 West Park Walk, E20 | 34 minutes from Chandni
Bottle Apostle is a lovely independent wine retailer where you get expert advice and the chance to try before you buy using self-serve enomatic wine ... More
Education near Chandni
London > Education > Adult Education
121 Roman Road, Tower Hamlets, London E2 | 3.5 miles from Chandni
For 30 years Four Corners has been training, producing and supporting the full range of film and photographic requirements. Services on offer include filmmaking facilities and ... More
London > Education > Career Services
27B Dalston Lane, E8 | 3.8 miles from Chandni
Connexions is a countrywide information and advisory service for 13-19 year olds and people with special learning needs or disabilities under 25. They offer an array ... More
London > Education > Evening Classes
10 Sunbury Workshops, Swanfield Street, Shoreditch, London E2 | 4.4 miles from Chandni
Learn some crafty manouveurs at Making Space where classes on mask making, glass engraving, pyrography (burning patterns into wood or leather), chainmail knitting, and book hollowing ... More
Leisure near Chandni
London > Leisure > Parks and Gardens
Celebration Avenue, E20 | 26 minutes from Chandni
Victory Park sits in the heart of East Village, acting as a 'green lung' for the east London neighbourhood created after the 2012 London Olympics where ... More
London > Leisure >
Queen Elizabeth Olympic Park, E20 | 27 minutes from Chandni
Four cycling venues in one, the Lee Valley VeloPark within the Queen Elizabeth Olympic Park includes the Olympic Velodrome track, the re-modelled Olympic BMX Track, one ... More
London > Leisure > London Olympics Venues
Olympic Park, Stratford, E20 | 28 minutes from Chandni
The iconic Pringle-shaped Velodrome in the north of the Olympic Park at Stratford, a state of the art stadium built for the London 2012 ... More
Pubs near Chandni
London > Nightlife > Pubs
116 High Road, E15 | 18 minutes from Chandni
A pub with a golden palm tree and eight private heated cabanas in the garden, The Leyton Star is the revitalised ... More
London > Nightlife > Pubs
52-54 Celebration Avenue, E20 | 26 minutes from Chandni
A 'beer shop' with all-day dining , neighbourhood in East Village is the kind of place all neighbourhoods should ... More
London > Restaurants > Gastropub
47 Stratford Broadway, E15 | 32 minutes from Chandni
A gastropub East End style - and that doesn't mean organic, corn-fed jellied eels and a gang of smoke ventilation engineers. The team behind King Eddie's ... More
Nightlife near Chandni
London > Nightlife > Bars
Queen's Yard, E9 | 2 miles from Chandni
A craft brewery and pizzeria overlooking the canal, Crate Brewery offers a unique location to enjoy a pizza and a pint in a former print factory ... More
London > Nightlife > Bars
Swan Wharf, Old Ford Lock, E3 | 2.2 miles from Chandni
A pop up private members' club in a former Victorian stable in Swan Wharf, a 100-metre walk from the Olympic Park, The Fringe is the place ... More
Meeting venue hire near Chandni
London > Business > Venue Hire
23-27 Wadeson Street, Hackney, London E2 | 3.5 miles from Chandni
... More
London > Business > Venue Hire
Royal Victoria Dock, E16 | 3.9 miles from Chandni
A stunning architectural achievement, gigantic amounts of floor space accompanied by shops, bars and hotels, London's purpose built exhibition centre, opened in 2000, is an exciting ... More
London > Business > Venue Hire
No.1 Warehouse, West India Quay, Hertsmere Road, Tower Hamlets, London E14 | 4.2 miles from Chandni
In keeping with its waterside location, the Museum of London Docklands focuses on the history of the East End with particular emphasis on the River Thames ... More
Also in High Road
Our recommendations in the same street as Chandni
Hotels Near
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Sseasonal, modern European dishes, with a nod to the flavours of the Indian Ocean from ...More
Sub Cult
Street food sandwiches by Sub Cult, famed for its USA deli-style sub rolls, are served ...More
Fantastic Mr Fox
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A playful re-telling of Roald Dahl's popular tale, Fantastic Mr Fox, by Maria Aberg who ...More
Concerts | On 27th September
Fresh from her storming Glastonbury performance, Lorde comes to Alexandra Palace promoting long-awaited new album ...More
Opera Holland Park Open Day
Concerts | On 23rd July
Part of the Opera Holland Park season, the ...More
Ultimate Aqua Splash
Sporting Events | From 24th July
Slide from the diving platforms used in the 2012 Olympics, take on the floating assault ...More
Best Things to do in London for an Adventure
Kidult thrill seekers looking for an adrenaline ...More
Italian Pizza Masterclass with Francesco Mazzei
Foodie Events | From 23rd July
The Peroni Ambra Terrace - popping up at ...More
Theatre | From 7th August
This is the last chance you have to see the critically acclaimed Wonderland, as Frank ...More
Dino Snores For Grown-ups
Exhibitions | On 4th August
Usually aimed at children, the Natural History Museum extends its late night Dino Snores event ...More
Wanderlust 108
Exhibitions | On 23rd September
Producers of the largest yoga lifestyle events in the world, Wanderlust present the world's only ...More
Hamburg On Tour In London
Concerts | From 20th October
Exporting the very best Hamburg has to offer, Hamburg on Tour is coming to London this October. Europe's innovative and culturally diverse hot spot, Hamburg has risen up the city break must visit top ten ...More
For their first restaurant outside of Denmark mother owners David Biffani and Nick Pound have ...More
Breddos Taqueria
More than double the size of the original, the 84-cover Breddos Taqueria on Kingly Street ...More
Design Frontiers
Exhibitions | From 18th September
More than 30 of the world's leading designers, including the director of design at Jaguar, are taking part in Design Frontiers, a group exhibition at Somerset House showing work which challenges and breaks through frontiers. ...More
Yeomanry House
An army careers centre, Yeomanry House is home to AFCO London....More
Suvlaki Shoreditch
Traditional grilled meat skewers cooked over hot coals are the signature dish at Greek restaurant ...More
Long Arm Pub
The flagship site of the Long Arm Pub & Brewery this Worship Street pub comes ...More
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There's no need to leave town when you're in search of sand, feel the ...More
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Hotels near Chandni
From cheap budget hotels to luxury 5 star hotels see our discounts for Chandni hotels, including Holiday Inn London - Stratford City from 80% off.


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Parachute Free
by: Ominous Game Labs • 872
All Android applications categories
by: Ominous Game Labs • 872
Published: 2012-11-19, by Manu Galvez.
Premise is simple: level begins with a ParaMan falling down. Your goal is to guide them towards the landing platform while avoiding platforms and collecting power-ups.
The guy is tilt-controlled, what actually is the strongest point of the game. Tilt the phone left/right to move and toward/away from you to speed-up/slow-down.
Try to collect as many power-ups as you can since some of them help you reaching faster or safer the landing platform and, some of them are extra points packages. There are up to 4 free levels in this Lite version and both local and online leaderboards (for that 4 levels) to show off who's really mastering the game.
Graphics won't amaze you: simple and childish graphics as we're used to see in casual games. Tilt-controls are quite accurate which is what makes the difference in this game. Worthy.
Tags: parachute widget , hungry shark htc legend , graphic parachute , parachute games free , 101 in 1 games paracute , parachute landing soundeffect
by Manu , Appszoom
Nov 19, 2012
from 872 reviews
by Sheila Durston
Feb 02, 2016 | "Good"
by Dj Cruz
Mar 01, 2015 | "OK"
I do not like it
by Andy Ryan
Nov 03, 2014 | "Awesome"
Very simple
by Joe Ho
Sep 01, 2014 | "Poor"
Not working
by Rahmat Aiman
Aug 10, 2014 | "Awesome"
by Deepak Modasiya
Dec 15, 2013 | "Great"
Mast h bose
Download Parachute Free
Free - V1.2.7 - 5M
Share this app


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Fantastic Mississippi River Cruise: American Queen Cruise Review by dijoen
Overall Member Rating
Fantastic Mississippi River Cruise
Destination: USA
Embarkation: New Orleans
The food was very good and plentiful, cookies were always available. We did learn that in 2012 the food service was outsourced and in 2013 it has been brought back in house. This has clearly made a significant difference as everything was well scripted.
The cabin, we stayed in an A category cabin on the 5th deck, was more than adequate. Clean, bright and quiet.
The nightly entertainment was great and finished off a great day that tended to be exhausting if you take in all that is offered.
People have commented that an American crew may not provide a high level of service. This clearly is wrong as we enjoyed perfect service from all of the crew who were well trained and always ready to help. All of the More advertised promises in the literature about the cruise were completely fulfilled.
We will return to cruise the complete Mississippi and all the other rivers the American Queen visit. Less
Published 03/28/13
Cabin review:
Read Cruise Critic's American Queen Review >>
Lots of attention to the small...
Cruising the coast of Maine
Beautiful Area
It Was an OK Experience
Absolutely a Great Cruise Down...
Great ship - poor shore excurs...
Pleasant week on the Columbia ...
Beautiful Area
It Was an OK Experience
Sponsored Links


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Explore Wood Candle Holders, Candle Holder Set and more!
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IMAX Levi Wood Candle Holder
Set 5 Colorful Wood Candle Holder Distressed Finish
Shop Joss & Main for your 3-Piece Bedford Candleholder Set. Indulge the interiors of your living area with a touch of classical style and bring home the One Allium Way wooden candle holder (set of 3).
Found it at Wayfair - Woodland Imports Wood Candlesticks (Set of 3)
Asst. of 5 Delila Candleholders, Gold | End-of-Year Warehouse Sale | One Kings Lane
Antique White Floor Candle Holder, 36 in. | Kirkland's
Safavieh Iris Table : Target Mobile
Art Deco A SET OF BATH TAPS American, circa 1925-35 marked 'sterling silver - silver mounted', pale green guilloche enamel Lot | Sotheby's
Adorned with raised fleur-de-lis details and a cream finish, this set of earthenware canisters brims with Provencal style for your kitchen or powder room. ...
top 6 trending nude neutral wedding color ideas #weddingcolors #weddingideas


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Apr. 6 / 6pm
Heights HS PTO Calendar
Apr. 6 / 6pm
April 5SAT for JUNIORS
April 14Spring Holiday
Keep up to date with everything happening at Heights HS by regularly visiting the Heights HS PTO calendar page.
Apr. 6 / 6pm
Information about the events and news sponsored by the Heights HS PTO in Houston, Texas.
Heights High School
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Houston, TX 77008
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