text:
Marian Martin Mail Order 9288; ca. 1950s; Unprinted Pattern. Misses' Sleeveless Dress with princess fitted bodice, lowered waistline and straight skirt, plus Jacket.
Links to reviews/blog posts Edit
Sources/Vendors Edit
Gallery Edit
[insert your photos of this pattern made up]
Wishlist Edit
[insert your username, and make sure your preferences allow for people to contact you via e-mail. Do not list your email address here!]


text:
About Us
America Rising Squared (AR2) is a 501(c)(4) organization focused on advancing conservative principles through cutting-edge research, rapid response communications, a nationwide tracking network, and digital platforms.
AR2 is dedicated to promoting the principles of freedom, limited government, free enterprise, and a strong national security, and pushing back against big government liberal policies and the special interests that support them.
AR2 is a unique platform for engaging in the public policy debate – combining issue, investigative, and field research, rapid response communications, a "war room" for comprehensive media monitoring, a nationwide video tracking network, as well as digital platforms and social media operations.
Since its launch in 2014, AR2 launched a series of initiatives:
CoreNews – Holding the Environmentalist Left accountable for their hypocrisy and extremism
CoreNews Texas – Pushing back on the national Environmentalist Left's efforts to end fossil fuel production in the Lone Star State
Labor and the "Fight for $15" – Exposing big labor's job-killing "Fight for $15" minimum wage campaign and the Obama Administration's assault on free enterprise through the radical actions of its NLRB and Labor Department
Supreme Court Vacancy – After the passing of Justice Scalia, AR2 has helped lead the opposition to President Obama's attempt to cement his liberal legacy with another Supreme Court appointment
Missouri Rising – AR2's first state initiative focused on Right to Work and other key issues in the Show Me State
Who we are
Brian Rogers, Executive Director: Brian Rogers is Executive Director of America Rising Squared (AR2). Prior to joining AR2, Rogers served as Communications Director to U.S. Senator John McCain (R-AZ), and previously served as Deputy Communications Director and Research Director for McCain's 2008 presidential campaign, and held senior communications and opposition research roles on multiple statewide campaigns across the country, at the Republican National Committee and the National Republican Senatorial Committee.
Jeremy Adler, Communications Director: Jeremy Adler is Communications Director of America Rising Squared (AR2). He previously served as a regional press secretary for Marco Rubio's 2016 presidential campaign. Before that, he worked as a member of America Rising's Senate research team and also was a part of Scott Brown's 2014 New Hampshire Senate campaign.
Jared Wheeler, Research Director: Jared Wheeler is Research Director of America Rising Squared (AR2). Wheeler previously served as Deputy Research Director at the Republican National Committee. Earlier in his career, he worked in the U.S. House of Representatives, the National Republican Congressional Committee and VA Victory.
Nathan Brand, Press Secretary: Nathan Brand is Press Secretary of America Rising Squared (AR2). Before coming to AR2, Brand served as the Rapid Response Director for Kelly Ayotte's Senate campaign in New Hampshire. He also was a member of the communications team on Marco Rubio's 2016 presidential campaign. Brand is a proud graduate of Hillsdale College.

subset: commoncrawl


text:
Match Details
- The Cricket Association of Bengal, DR BC Roy Club House, Eden Gardens, Kolkata - 700021
Player of the match
TV Umpires
Reserve Umpire
Match Referee
Scorecard Summary
- RN ten Doeschate1/7 (1.0)
- Shakib Al Hasan1/25 (4.0)
- YK Pathan72 (22)
- RV Uthappa41 (30)
Yusuf Pathan - 72 off 22. That was unbelievable hitting. He's still pumped, waving a stump about in jubilation.
Rishabh Prajapa: "This innings has now paid off the entire money KKR had paid for the four years to Yusuf."
SKD: "Man of the match: Anirudhha Srikanth."
Suman Dustakar: "In Excitement you also forgot that karan sharma took 4 wkts :)" Totally did.
Darren Sammy: "I wish I could hit like that. Credit to Yusuf. Phenomenal hitting. We've not been consistently good enough this tournament."
Sudipta: "Netherland revisited and relieved. Qualified withan over to spare. Robin doing a Myburgh and Yusuf doing a Cooper here."
Nandakiran N: "Binary form of batting is Yusuf Pathan. Play a blinder or blindly get out. :P"
Karthik: "What a fine margin between being a hero and a zero -quite literally in this case! Pathan dropped on 0."
saurish: "what the hell happened,i had gone to have dinner for 15 mintues..returned and games over :o"
faraz681: "Yusuf hit Dale Steyn for 26 in an over....move over Dhoni..."
John Paul: "Yusuf On, Comments Gone"
Robin Uthappa: "There was a massive belief that we would play in the semi-finals here, when I was walking past Danny before the game he asked me whether we would make it, I told him, I know we'll make it." Uthappa also has plenty of praise for the Eden Gardens crowd.
Chennai Super Kings tweet: "One more game! One more reason to #whistlepodu!"
Sharma: "Thank you YK Pathan for understanding I have exams to revise for and finishing the match early."
Ishan Jain: "Steyn's reputation has taken a serious pounding this season. Hope he recovers soon from this traumatic season. He is the arguably most threatening fast bowler of this era."
Presentation ceremony:
Gautam Gambhir: "I have never seen this sort of innings in my career, unbelievable hitting, I can only dream of it, but I saw it in reality. We always wanted to finish it in 15.2 overs, this is what we play cricket for. We didn't start well up front bowling, we dropped some crucial catches, but this is not the time to talk about negatives, I have never seen such an innings."
Man of the Match is Yusuf Pathan: "I hit form at the right time. It feels great when the ball hits the middle of the bat and travels a long way. My hundred for Rajasthan was a good knock but I got run-out and we couldn't win the match, but this time we won, this feels like my best innings."
Well, I need a breather after that unbelievable game. I'm off, sorry couldn't post more comments when Yusuf was going bonkers. See you tomorrow. G'night.
7 needed off 7
END OF OVER:14 | 12 Runs 1 Wkt | KKR: 154/6
- SA Yadav6 (3b)
- R Vinay Kumar0 (0b)
- KV Sharma4-0-38-4
- DW Steyn4-0-43-0
Steyn taken apart
Number of runs off <i>that</i> Steyn over - his most expensive in the IPL
Toeing the line
No. of no balls that have been hit for a boundary this IPL. Today was the only time a batsman hit consecutive no balls for boundaries across seasons
Steady going
No. of balls Uthappa has faced from Steyn without getting dismissed, in the IPL. Has scored 34 runs
Not beyond reach
The highest any team has scored in 15.2 overs while chasing at the Eden Gardens, Kings XI against Knight Riders in 2010
Score to which Sunrisers Hyderabad need to restrict Knight Riders to if they want to get above Rajasthan Royals on net run-rate
Target locked
No. of balls by which Knight Riders need to secure victory to take second place on the points table
Biff or dot
Naman Ojha today was the second-highest score in the IPL with only boundaries, Jayasuriya's 36 against Deccan Chargers in 2008 is the highest.
Narine factor
Dhawan's strike rate against Narine. He has also been dismissed once by the bowler in 14 deliveries before this match
The break up
Shakib's economy rate against left-handed batsmen in the IPL, against right-handers he goes down to 6.12
No. of wickets taken by Knight Riders' spinners this seaon, the most by any team
Dhawan on top
Shikhar Dhawan's strike rate against Morne Morkel, has hit 52 runs in 28 balls without getting dismissed
No. of times Morne Morkel has dismissed David Warner in T20s. He only has 17 runs in 16 balls
Repeating history
No. of consecutive wins Knight Riders have currently, equals their longest winning streak in the IPL
The equation
No. of balls in which Kolkata Knight Riders need to win provided the target is between 130 and 200 to secure second place


text:
Shipshewana Office Collection
Complete your office suite with our Manitoba Lateral File Combination cabinet. The false front drawers on the left side pull out into a large deep drawer to hold letter sized files while the drawers on the right side are two storage drawers. The shelf on the left side pulls out so that you can store a printer or small fax machine that can be accessed easily. With an adjustable shelf on the right you can move it up and down to accommodate the things you want to display. If you have any questions on this product or any of our other products give one of our furniture specialists a call today.
Shown in Brown Maple Wood with Rich Tobacco OCS-228 Finish
© Copyrights 2003 - 2017 DutchCrafters All rights reserved.


text:
Bounce and glide through the world to rescue the Frizzles! This sequel challenges you to collect gears, keys, and tiny creatures. You can jump on tree branches and certain birds. Save your friends for maximum points in Frizzle Fraz 2!
Frizzle Fraz 2 is one of our selected Skill Games. Also very popular on this website right now are Fireboy and Watergirl 5, Home Sheep Home 2: LL, Home Sheep Home 2: LU and Piggy in the Puddle 2.
Report game as broken
Cancel report of broken game


text: Starting with Heavy allways watch out for em.On with the demoman nice guy to get kills with him with nice a granade lancher and his sticky bombs but be careful with his beer bottle.Oh i forgot about the Heavy spin his minigun round and round hes got 200 hp but his weakness is his back side.Next up the spy hes more divous than your ex.Hes the best trained spy in all of france wepons kinfe,watch,revolver,and the disguse case.EAT MY DUST hes fast as lighting its the scout his pistol is good for hiting enemys far range.His scatter gun is really good for doing damge.BONK home run baby the scouts bat does damge so don`t think the bat sucks.streaming egals! its him its the soldier kill enemys with his rocket lancher other wepons shovel, shotgun.MEDIC! someone you need help from all the time.his needlegun to give your enemys a tase of your medice.


text:
Why cant i upload any images in my posts ? v,v I read i needed 10 posts and i have them so?
and also , How do i make my image larger by my posts? i see people with images larger then mine ,
Click Here to Become a MPGH Premium Member by Donating $25 or More.
If anyone claims to be me via MSN/Skype/Email or any other source outside of MPGH private or visitor messages, IT'S NOT ME!
They are trying to trick or scam you. Report them immediately and PM me here for verification.
"Dont confuse my personality with my attitude. My personality is who I am. My attitude depends on who you are." Frank OceanModerator: 5/2009-10/2009 | GMod: 10/2009-10/2010 | Staff Administrator: 10/2010-Present
Stupid/Pointless Private messages = SPAM, SPAM = BAN.


text:
I was starting to comment too much on the Life, Rare and fragile post; I couldn't help myself. Thought if people wanted to discuss any of the ideas brought up there more in depth they could do it hear.
No announcement yet.
Life, Rare and Fragile
new posts
I agree Acmebike. I wonder if the SAD has anything to do with the growing need for reproductive medicine?
The more I see the less I know for sure.
-John Lennon
WOW that's incredible!!! Congratulations Acme!
The more I see the less I know for sure.
-John Lennon
Shine, It could be. Sarcasm doesn't transmute through text very well. It is hard to determine what "just saying" signifies. If that was supposed to be the sarcastic qualifier I didn't interpret it as such.
Now I do want to call Poe's Law, I can't tell!
This thread is reminding me of the Pottenger Cat experiment
I am glad there is such a community of critical thinking people here... typically I am fighting a solo, uphill battle trying to reason against both CW and religious beliefs.
Mark's post beautifully illustrated my view on life and why there is no need for a god (or gods) in my life to find enrichment, meaning, or happiness.
I do believe we are naturally curious, and from that curiousness religion spawned to fill the unknowns. I also believe that at any given time there are things we will never fully understand and really have no need to understand (i.e. creation of the universe)
I think people that hold the creationist belief feel life is degraded by not being the center of the universe, not being the children of god. If they could simply grasp what Mark said, I think they could go on living and appreciating/enjoying life without their bibles and baby jesus.
Ridgeback, all religious/spiritual people are not un-scientific. There are some who can grasp science and still hold fast to faith in something bigger than themselves. Yes, it is annoying to have people bring "peusdoscience" into a debate and expect it to be swallowed, but that doesn't mean those who do understand science have the right to be judgemental and bigotted.
I think mindless adherance to any group, whether it be religious, or otherwise is dangerous. Fundamentalism comes in many forms. I have encountered fundamentalist environmentalists, parenting fundamentalists, and even PRIMAL FUNDAMENTALISTS!
I guess I just see a difference in people who follow organized religion without questioning or thinking for themselves, and those who have found a spiritual path that they feel gives them some peace, grace and a greater compassion for others.
I think we should have respect for people's spirituality, and even atheism can be considered a type of spirituality. Tolerance and respect shouldn't be too much to ask on a board with so many enlightened educated thinkers.
The more I see the less I know for sure.
-John Lennon
Thanks for those links. I never heard of Francis Pottenger or his famous cat study before. It was interesting to learn he was a contemporary of Weston Price!
To me, prima facie, the results demonstrate how potentially destructive an "unnatural" diet can be to an organisms health. One thing I wonder: could these (or have these) results be interpreted that raw meat is more beneficial for human health? Because one difference between cats and humans is that humans have been cooking food for a very long time (I think at least 100,000 years?) and cats never have...until humans started feeding it to them.
I don't want to sully you post by attaching ancillary ideas to it but what you said reminded me of a Ralph Waldo Emerson quote, "Foolish consistency is the hobgoblin of little minds." I don't think any group of thought is immune to falling into 'foolish consistency'.
considering that most of modern science has its origins in Christians seeking to logically and scientifically explain God's creation, i find the fundy-atheist thing just irritating.
the whole 'omg there is DEFINIATELY NOT GAWD' is just ridic. the data doesn't dictate one way or the other whether the Christian God exists or not. a correct scientific response would be agnosticism, not rabid atheism and accusations that your brain musta fallen out if you're Christian and like math and science.
issac newton was working within a Christian intellectual tradition when he formulated much of what we understand to be modern physics. people forget so much of what religious faith contributed to science before the last 2-3 generations. people have such tiny, short memories. it's fascinating how so many really do have faith that everyone before the 60s was Just Plumb Dumb, when the scientific evidence is actually that our brains are 20 percent smaller since the dawn of neolithic eating, half of that an artifact of modern agriculture (the last century or so).
it is wonderfully strange how so many evolutionary rabid atheist primal/paleo types actually kinda believe those same paleo ancestors were just stupid but they are much smarter.
I am deeply shocked and saddened at the level of irrationality present in this post's comments. (I am referring to the comment board, not this forum thread.) I seriously just read a response to one of my comments using the "discovery" of dinosaur footprints found alongside human footprints to refute my defense of the scientific method. I was then told:
Just look at how butthurt and hostile the people on your side of the argument have gotten just during the course of these comments.
I really don't understand. Most of the name-calling that I have witnessed in the course of the post's comments has been directed towards the "self-righteous, ignorant, intolerant" non-believers rather than from.
Religion was introduced into the conversation by the third post promoting young-Earth creationism. (Whether or not this was a Poe remains unknown as the poster has remained silent since.) Rightfully so, this comment was completely lambasted as indubitably false. However, because this represents a fundamental belief of many people, suddenly feelings were hurt and tempers were riled.
This is the problem with belief: because it is not based upon any evidence, any criticism of a belief threatens to erode its unsubstantiated foundations. This is, understandably, very uncomfortable for the believer. Presentation of facts which contradict the faithful edicts cause emotional distress and are construed as personal attacks. The issue becomes an emotional maelstrom as believers and non-believers alike resort to ad hominem attacks and impassioned responses.
Even though I have rambled on far beyond the realm of TL;DR I would like to make one more point. There seems to be a confusion between atheism and anti-theism. (I suppose the latter is sometimes called "militant atheism.") At its core, atheism represents a rejection of unsubstantiated belief systems, an insistence upon evidence, and a promotion of critical thinking and healthy skepticism. It does not claim that there is no god; rather, atheism simply does not accept the claim that there is a god based upon lack of evidence. Anti-theism, on the other hand, actively states that there is no god.
This is the fundamental difference: inactivity and activity. Atheism is inactive; it is simply a rejection of a belief system and does not actively make any claims about the nature of the universe. Anti-theism is active; it not only rejects a belief system (theism), it actively promotes the belief that there is no god.
Although it is an extremely well-worn analogy, consider the existence of fairies. Let's suppose that a belief in the existence of fairies is called "fairyism," and all believers in fairies are called "fairyists." However, because there is no evidence for fairies, you are hard-pressed to find anyone who actually believes in their existence. Therefore, the majority of people would reject fairyism and be labeled as afairyists.
Even though most of us (hopefully) do not accept the belief in fairies, is the label of "afairyist" truly relevant? Does it really make sense to define ourselves by what we don't believe in?
Although most people are afairyists, how many spend their time actively claiming that there are no fairies? Unless fairies are brought up in the course of conversation, how many people are likely to spout off an anti-fairy agenda? Probably not many. However, if the claim of fairies' existence is mentioned, most people will be provoked to then refute this claim and possibly lapse into anti-fairyism.
This is the mechanism by which atheists are construed as anti-theists. The constant interjection of theism into debate prompts the non-believer to assert their rejection of theism and, in the process, is often misunderstood as making an independent claim.


text:
Home >> Undergraduate Degrees >> College of Arts & Sciences >>
Following is one suggested four-year degree plan. Students are encouraged to see their advisor each semester for help with program decisions and enrollment. Students are responsible for reviewing the Program of Study Requirements.
*See Appendix
A Core Curriculum and Optional Course Information.
** See Appendix C for approved list of minors and requirements.
1Math elective, select 3 SCH from
any math course above the level of College Algebra.
2Communication, select 3 SCH from ENGL 2311, HUM 2301 or SPCH 1311.
3Geography electives, select 6 SCH from GEOG 3350, GEOG 3352, GEOG 3353.
4Research Methods, select 3 SCH from CRIJ 3305, PSCI 3301 or SOCI 3305.
5Restricted electives, select 12-13 SCH from ECO 1301, ENSC 3401, GIS 2301, PSCI 3345, SOCI 4310, or SOCI 4317.
Actual degree plans may vary depending
on availability of courses in a given semester.
Some courses may require prerequisites not listed.
Home - General Information - Student Services -
Academic Undergraduate Regulations -
Undergraduate Degrees - Academic Graduate Regulations -
Graduate Degrees - Course Descriptions - Faculty -
Appendix A - Appendix B - Appendix C - Appendix D


text:
A semantic proteomics dashboard (
The International Conference on Intelligent Biology and Medicine (ICIBM): Systems Biology
The International Conference on Intelligent Biology and Medicine (ICIBM)
One of the primary challenges in translational research data management is breaking down the barriers between the multiple data silos and the integration of 'omics data with clinical information to complete the cycle from the bench to the bedside. The role of contextual metadata, also called provenance information, is a key factor ineffective data integration, reproducibility of results, correct attribution of original source, and answering research queries involving "What", "Where", "When", "Which", "Who", "How", and "Why" (also known as the W7 model). But, at present there is limited or no effective approach to managing and leveraging provenance information for integrating data across studies or projects. Hence, there is an urgent need for a paradigm shift in creating a "provenance-aware" informatics platform to address this challenge. We introduce an ontology-driven, intuitive
The SemPoD platform, currently in use at the Case Center for Proteomics and Bioinformatics (CPB), consists of three components: (a) Ontology-driven Visual Query Composer, (b) Result Explorer, and (c) Query Manager. Currently, SemPoD allows provenance-aware querying of 1153 mass-spectrometry experiments from 20 different projects. SemPod uses the systems molecular biology provenance ontology (SysPro) to support a dynamic query composition interface, which automatically updates the components of the query interface based on previous user selections and efficientlyprunes the result set usinga "smart filtering" approach. The SysPro ontology re-uses terms from the PROV-ontology (PROV-O) being developed by the World Wide Web Consortium (W3C) provenance working group, the minimum information required for reporting a molecular interaction experiment (MIMIx), and the minimum information about a proteomics experiment (MIAPE) guidelines. The SemPoD was evaluated both in terms of user feedback and as scalability of the system.
SemPoD is an intuitive and powerful provenance ontology-driven data access and query platform that uses the MIAPE and MIMIx metadata guideline to create an integrated view over large-scale systems molecular biology datasets. SemPoD leverages the SysPro ontology to create an intuitive dashboard for biologists to compose queries, explore the results, and use a query manager for storing queries for later use. SemPoD can be deployed over many existing database applications storing 'omics data, including, as illustrated here, the LabKey data-management system. The initial user feedback evaluating the usability and functionality of SemPoD has been very positive and it is being considered for wider deployment beyond the proteomics domain, and in other 'omics' centers.
Though many molecular system biology research centers now have significant infrastructure in terms of instrumentation to acquire 'omics datasets, most of these datasets end up in study-specific data silos. Specifically, more than 50% of data being generated in laboratories are stored in local lab servers
In which subset of patients and/or clinical stage is signaling pathway X most activated?
Extending the above scenario, the researcher may consider that although activation of pathway X is altered in a mouse model of disease Y, it is not clear that this is also the case in humans. Thus, if the researcher acquires datasets from several different cohorts of patients with disease Y, she might ask:
Is the cognate pathway X also important in human?
At present, there is no informatics infrastructure in the CPB that is capable of supporting these categories of queries. In addition, the lack of an effective query platform is also a key reason that once the 'omics data has been acquired, analyzed and interpreted, the data is typically archived and serves no further process. This is important issue both in terms of maximizing the return on research funding and also ensuring that the value of 'omics data can be significantly increased if that data is carefully integrated into a growing corpus of data that can then be re-used in different contexts. For example, a researcher with a long-standing interest in disease X has acquired multiple large-scale proteomics and transcriptomics datasets over several years. In response to a newly published finding that Single-Nucleotide Polymorphism (SNP) in gene Y are associated with disease X, the researcher now wants to query all of her legacy data and ask
Are there any new associations between gene Y and disease X?
In general, these types of queries are difficult to perform because they integrate several types of information, including biological annotations from outside sources.
Provenance-aware integrated query environment
The role of contextual metadata describing the experimental conditions, for example sample type, instrumentation, sample preparation, and statistical measures, is being increasingly noted as a key factor in managing translational research data
In addition to incorporating provenance metadata in medical informatics platforms, there is a well-recognized need for an intuitive and powerful query interface that can be directly used by researchers. Frequently, analysis and querying of 'omics datasets requires expertise that may not be available to many translational laboratories. For example, in a recent survey in the Science journal about 57% of researchers have either no support for data analysis or are dependent on others for managing experiment data
The SemPoD platform is designed to address these challenges through use of provenance informationintegrated with a visual, ontology-driven, integrated query environment.
We use two principal proteomics workflows used in the CPB as exemplars to describe the design and implementation of SemPoD, namely:
1. The first workflow is affinity-purification mass-spectrometry (AP-MS) workflow that enables the identification of specific protein complexes, thus identifying proteins that are associated with one another.
2. The second workflow is the shotgun expression proteomics that identifies and quantifies proteins in an unbiased manner from cells or tissues of interest.
Together, these two workflows account for approximately
SemPoD was developed using agile software engineering methodology for rapid and iterative development in close consultation with the users. The agile engineering approach was combined with the Ruby-on-Rails web development framework that uses a Model-View-Controller (MVC) architecture pattern. The MVC pattern involves a strict separation of the application logic from the user interface, which allows SemPoD to seamlessly adapt to changing requirements of translational research studies, with a consistent query environment (Figure
Figure 1
The SemPoD Architecture with the SysPro ontology
The SemPoD Architecture with the SysPro ontology. Figure shows the high-level architecture of SemPoD web-based dynamic query interface that uses the Model-View-Controller (MVC) architecture design pattern. The figure shows how SemPoD integrates 'omics datasets from heterogenous data sources like Proteus LIMS using a ontology-driven, provenance based approach. The figure also shows how SemPoD interfaces with data analysis and viewing applications like Labkey.
SemPoD leverages the SysPro ontology as the core resource to support various query functionalities, including "smart filtering" for reducing user effort in composing complex query patterns.
The systems biology provenance (SysPro) ontology
At present, the provenance metadata associated with the different stages of the proteomics workflow at CPB is not collected in a systematic manner. Often, the provenance metadata is stored as hand-written notes in a lab book and is not immediately available for query and analysis of the proteomics dataset. Further, any modification in the experiment protocols or related experiment metadata information makes it difficult to correlate or integrate data from previous runs with new datasets. The use of a variety of terms to describe provenance increases terminological heterogeneity across different projects and makes it difficult to effectively integrate datasets.
Hence, the SysPro ontology was developed to model experiment metadata by re-using and extending existing minimum information reporting guidelines defined by the 'omics community. Several "minimum information" reporting frameworks have been developed and are now part of the minimum reporting guidelines for biological and biomedical investigations (MIBBI) project
The PROV-O is a reference ontology being created by the W3C provenance working group to facilitate provenance interoperability with a set of common provenance-specific classes and relationships. The PROV-O terms can be extended by various domain-specific applications, such as SemPoD
Figure 2
The SysPro class hierarchy and instances for class 'Cell Line'
The SysPro class hierarchy and instances for class 'Cell Line'. This figure shows the hierarchy of classes in the SysPro ontology. This figure is a screenshot taken from Protege tool that was used to create the SysPro ontology. On selecting a class, for example 'Bait Type', the instances of this class is shown on the right pane, namely 'Endogenous', 'Exogenous', 'KnockIn', 'Tagged' and 'Untagged'.
The SysPro ontology also facilitates cross-linking of 'omics data with a variety of related genomics and clinical datasets, which are annotated with domain ontologies
To allow experiment data generated in CPB to be linked to external datasets at UniProt (for protein data) and GeneDB, inter-ontology mappings between SysPro, GO, and the Protein Ontology (PRO)
Figure 3
The mapping process of SysPro ontology terms in the LabKey
The mapping process of SysPro ontology terms in the LabKey. This figure shows the steps for configuring a data source for querying the underlying 'omics data. A data source can be dynamically configured by mapping the SysPro ontology classes to the underlying database. After configuration, a query can be composed using the query builder and submitted on this data source.
The SemPoD query environment
SemPoD consists of four main components, namely (1) the SysPro ontology browser, (2) the integrated query builder, (3) the result explorer, and (4) the query manager (Figure
Figure 4
The four constituent modules of SemPoD
The four constituent modules of SemPoD. This figure shows the four main components of SemPoD namely the hierarchical
SemPoD ontology browser and query builder
The SemPoD query builder component (Figure
Figure 5
SemPoD Query Builder
SemPoD Query Builder. Query Builder is an intuitive interface that allows selection of query conditions from the SysPro ontology browser and create dynamic queries by selecting different logical connectives and parameter instances.
Figure 6
Screenshot illustrating the "smart filtering" feature implemented in the query builder
Screenshot illustrating the "smart filtering" feature implemented in the query builder. Smart filtering is an feature that enables effective selection of query parameter and their instances during query composition. Smart filtering updates the drop-down list for a selected query condition based on all the previously selected query parameters. The advantage of this approach is to eliminate selection of query parameters that will not bring any valid results.
The SemPoD query builder uses the SysPro ontology to support an advanced feature called "smart filtering" that dynamically updates the query interface in response to previous user selections. Figure
Further, the "smart filtering" feature leverages instance-level relationships defined in the SysPro ontology, which links only specific instance values with each other. For example, the "EPHB2" instance of class "Bait gene is associated with only "HCT116", which is an instance of class "Cell line". Hence, when the user updates her selection of "bait gene symbol" from "CTNNB1" to "EPHB2", the corresponding instance value for the "Cell line" is automatically updated to "HCT116" (Figure
Figure 7
Screenshot illustrating the use of property linking two instances for populating drop-down menus
Screenshot illustrating the use of property linking two instances for populating drop-down menus. Smart Filtering feature leverages instance-level relationships defined in SysPro ontology, which links only specific instance values with each other.
Figure 8
Use of property hadRole in the SysPro ontology linking classes Cell Line and Bait Gene
Use of property hadRole in the SysPro ontology linking classes Cell Line and Bait Gene. This figure shows an example of instance-level relationships between the Bait Gene and Cell Line classes defined in SysPro ontology.
SemPoD result explorer
The user can explore the results of their queries in the SemPoD result explorer (Figure
Figure 9
The result explorer allows users to link out to the underlying Labkey database
The result explorer allows users to link out to the underlying Labkey database. Query results are shown in separate tabs, one for each project. The experiment files are listed which then interface with underlying Labkey proteomics data.
SemPoD query manager
The user can also save their queries using the 'Save Query' button in the query builder interface(Figure
Figure 10
The query manager showing a list of queries with details describing date of creation and update
The query manager showing a list of queries with details describing date of creation and update. Query Manager shows the list of saved queries by differnet users. On expanding the row, the details of the query are shown.
SemPoD has been deployed at the CPB and has been in use for over 2 months. SemPoD was evauluated both in terms of systematic user survey and scalability for queries with different levels of complexity over increasing size of data.
User evaluation
The user evaluation was done in two-phases over a 4-month period, the first user feedback was collected after 2 months of deployment, and a second survey was conducted 4 months after initial deployment. The user feedback from each phase was used to update SemPoD and modified features were also evaluated in the subsequent user survey. The user survey consisted of 16 queries evaluating different aspects of SemPoD, including ease of query composition, navigability of the SysPro ontology terms, accuracy of result datasets, and presentation of data in the result explorer. The survey used a scale of 1-10 to measure response. For questions Q1-9 and Q12, the user response was recorded as a measure of "the difficulty level of query composition" with 1 representing "not difficult at all" and 10 representing "very difficult". For questions Q10, Q11 and Q13-16, the user response was recorded as a measure of "how informative, consistent, easy-of-use" with 1 represented "not useful at all" and 10 representing "very useful". Figure
Figure 11
Results of user feedback after 2 months and 4 months of SemPoD deployment
Results of user feedback after 2 months and 4 months of SemPoD deployment. User ratings for 2 surveys are shown for questions 1-16. Survey 1 was done after 2 months of deployment and survey 2 was done after 4 months of deployment.
SemPoD scalability evaluation
In addition to user evaluation, the scalability of SemPoD was also evaluated with respect to increasing complexity of queries, in terms of logical connectives used to compose the query, and sizes of data. Table
Table 1
Details of queries used to evaluate the scalability of SemPoD
Q1. Search proteomics experiments in any human sample
Organism = 'Homo Sapiens'
Q2. Search proteomics experiments for 'Embryonic stem' cell line in any human sample
Organism = 'Homo Sapiens' (OR) Cell Line = 'Embryonic stem'
Q3. Search proteomics experiments for Human samples with Cell Line 'Embryonic stem' or Pertubated with 0 Dosage in Cytosol Subcellular Fraction
Organism: 'Homo sapiens' (AND) Cell Line: 'Embryonic stem' (OR) Perturbation: 'Dose = 0' (OR) Subcellular Fraction: 'Cytosol'
Q4. Search Experiments for Bait Gene 'DNMT1' in AP-MS experiments or WNT3A perturbations in Bait Run Group for Cell Line RKO
Bait Gene Symbol = "DNMT1" (AND) Experiment Type = "AP-MS" (OR) Perturbation = "WNT3A" (AND) Run Group = "Bait" (OR) Cell Line = "RKO"
Q5. Search Protein Expression Experiments for T-cells Cell Lines for Drosophila melanogaster organism perturbed with 10 ng in treated cell cultures
Experiment Type: 'Protein Expression' (OR) Cell Line: 'T-cells (Boom)' (AND) Organism: 'Drosophila melanogaster' (AND) Perturbation: '10 ng' (OR) Run Group: 'Treated' (AND) Sample Type: 'Cell culture'
Q6. Search Experiments for 'POU5F1' Bait Genes for 'Embryonic stem' Cell Lines in AP-MS or 'Mus musculus' organisms that are not perturbated or endogenous cell cultures
Bait Gene = 'POU5F1" (OR) Cell Line = 'Embryonic stem" (AND) Experiment Type = "AP-MS" (OR) Organism = "Mus musculus" (AND) Perturbation = "Not Applicable" (OR) Sample Type = "Cell culture" (OR) Bait Type = "Endogenous"
Q7. Search Protein Expression Experiments or 'T-cells Cell Lines for Drosophila melanogaster organism for Tagged cell cultures not perturbated for APC Bait Genes and No vector control run groups
Experiment Type: 'Protein Expression' (OR) Cell Line: 'T-cells (Boom)' (AND) Organism: 'Drosophila melanogaster' (AND) Bait Type: 'Tagged' (AND) Sample Type: 'Cell culture' (OR) Perturbation: 'Not Applicable' (AND) Bait Gene: 'APC' (AND) Run Group: 'No Vector Control'
Figure 12
Results for queries with increasing complexity over two datasets and two servers
Results for queries with increasing complexity over two datasets and two servers. Performance evaluation of queries for increasing query complexity for the queries listed in Table 1.
The results clearly show that the total time for increasingly complex queries is relatively stable over the two datasets. Although there is notable difference in performance between the 20 GB and 50 GB datasets for the same query (Figure
The functionality of SemPoD query environment is primarily limited by the provenance and domain information modeled in the SysPro ontology. Hence, in the next phase of SemPoD development, we are modeling terms from additional metadata standards included in the MIBBI project. In addition, the SysPro ontology is being expanded to include concepts from GO and PRO to enable linking of genotype and protein data from external sources with CPB internal datasets. This allow researchers to query across genotype and phenotype data, including clinical information.
The manual mapping of SysPro ontology terms to the underlying database is an important challenge that can be addressed by creating semi-automated mapping techniques, which can define initial mappings through use of lexical matching and subsequently reviewed by researchers. Since automated schema mapping is still an open research problem, the involvement of researchers to manually verify the ontology-to-database mapping will ensure the accuracy of results in SemPoD. We plan to release the first version of the SemPoD codebase as a git hub open source project, which will allow other users and developers to review and use SemPoD in other 'omics center. Similarly, the first version of the SysPro ontology will be released for public use through listing at the National Center for Biomedical Ontologies (NCBO)
Many researchers routinely use several different proteomics workflows to study biomedical problems. Studies may use different cohorts of patients, different cell lines or different techniques, but their value for biomedical discovery is significantly increased if researchers can query across these different studies as well as integrate with legacy data. The SemPoD platform is an ontology-driven intuitive query platform that leverages provenance metadata for effectively addressing these challenges. The SemPoD platform features four components to facilitate query composition using existing experiment metadata standard terms through an integrated ontology browser, a result browser, and a query manager to store queries for subsequent re-use or sharing with other researchers. The evaluation results for SemPoD, both in terms of positive user feedback and scalability for complex queries over increasing size of datasets, show that SemPoD can successfully meet the informatics requirements for large 'omics' research centers.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
CPJ designed the SemPoD architecture, implemented the query builder and ontology frameworks, integrated the Labkey system to SemPoD, and wrote the manuscript. MXZ implementated the query manager and validated the results of queries. SSS created the SysPro ontology and contributed to the writing of the manuscript. RME defined the usecases of the SemPoD framework and validated the results in the deployment phase. GQZ contributed to the designing the system architecture and writing the manuscript. All authors have read and approved the final manuscript.
This research was supported in part by the PhysioMIMI project (grant#NCRR-94681DBS78) and Case Western Reserve University/Cleveland Clinic & CTSA Grant (grant#UL1 RR024989.) We also thank members of the Center for Proteomics and Bioinformatics for their help in evaluating SemPoD prototypes.
This article has been published as part of
Challenges and Opportunities
Integrating with integrity
Position Statement: Musings on Provenance, Workflow and (Semantic Web) Annotations for Bioinformatics
A unified framework for managing provenance information in translational research
Multimodal Integration of Disparate Information Sources with Attribution
Data Provenance: Some Basic Issues
Using Semantic Web Technologies for Representing e-Science Provenance
VISAGE: A Query Interface for Clinical Research
Promoting coherent minimum reporting guidelines for biological and biomedical investigations: the MIBBI project
The minimum information required for reporting a molecular interaction experiment (MIMIx)
The minimum information about a proteomics experiment (MIAPE)
PROV-O: The PROV Ontology (Working Draft)
Quality assurance in biomedical terminologies and ontologies
Gene ontology: tool for the unification of biology. The Gene Ontology Consortium
Framework for a protein ontology
Computational Proteomics Analysis System (CPAS): An Extensible, Open-Source Analytic System for Evaluating and Publishing Proteomic Data and High Throughput Biological Experiments
The National Center for Biomedical Ontology
The Ontology for Biomedical Investigations
Modeling sample variables with an Experimental Factor Ontology


text:
Tuesday, July 15, 2014
Quorum Business Solutions Acquired By Silver Lake Partners
Houston-based software developer Quorum Business Solutions, a developer of financial accounting, transaction, trading, and other software for the oil and gas industry, has been acquired by private equity firm Silver Lake Partners. Financial details of the deal were not announced. Quorum Business Solutions was previously owned by funds managed by The Carlyle Group. Quorum is led by Perry Turbes.


text:
Networking DELL X1026 gigabit switch/24x 10/100/1000 port/2xSFP 1Gb/Web Smart Management
DELL Networking X1026 gigabit switch/24x 10/100/1000 port/2x 1Gb SFP/Web Smart Management/NBD on-site
Easy management office network with Gigabit switch that offers features enterprise-class and intuitive graphical user interface to manage. This product is provided by so-called. Limited lifetime warranty on-site next business day.
The warranty applies to replacement parts for this equipment during the lifetime of the product, min. However, 3 years.
The guarantee conditional registration on https://www.onlineregister.com/dell/.
Repairs under this warranty service taking place anywhere throughout the country following working day.
It may not even be at the company headquarters - you may be on vacation, on a business trip, it does not matter.
Just call tel. Number 225 308 649, respectively. phone 778 440 533, serial number and report the defect and Dell already takes care of it.
basic specifications
- Number of Ports: 24 x RJ-45, 2 x SFP
- PoE: no
- Switch capacity: 52 Gb/s
- Routing speed: 38.7 Mp/s
- Power psu: 40 W
- Weight: 1880 g
pages manufacturer
Logistics Information
|Nr. of pcs in package
|Weight (kg)
|Dimensions (cm)
|Length (cm)
No reviews added yet.


text:
Have Honda Questions? Ask a Honda Repair Expert.
Hello I will help you,
This is the light on the dash or the lights out back?
If out back can you unplug the brake switch on the shaft of the brake pedal and see if they go out?
Sure, I will be here.
I know it is tough to get up there, if the lights turn off see if the plunger for the switch is resting on a solid piece and if you can push it up in the switch and make the lights go out.
You either have a hole where the plunger rests that needs to be filled with a stop, the nut is loose and the switch needs to be adjusted or te switch has failed.
let me know what you find


text:
July 24, 2017
Huang Wins Analytical Chemistry 2017 Young Innovator Award
Award recognizes contributions demonstrating exceptional technical advancement and innovation in the field of micro- or nanofluidics
July 20, 2017
Studying Superfunds: Duke Environmental Engineers Investigate How Super-Polluted Areas Affect Early Human Health
As Duke's Superfund Research Center receives another round of funding, environmental engineers discuss how their work helps us learn more about common pollutants.
July 18, 2017
Chakrabarty Named Chair of Duke Electrical & Computer Engineering
Chakrabarty, the William H. Younger Professor of Engineering at Duke, is an internationally known computer engineering innovator recognized for his pioneering work in lab-on-a-chip design automation, 3-D integrated circuits and wireless sensor networks.
July 17, 2017
Duke Students Create Robot for Amazon Challenge
Team heading to Japan for international competition of robotics experts
June 27, 2017
Duke Engineers' Virtual Reality App Wins IGNITE NC Reverse Pitch Competition
App promises key features for sharing educational virtual reality experiences
June 26, 2017
Air Pollution Casts Shadow over Solar Energy Production
First study of its kind shows airborne particles and their accumulation on solar cells is cutting energy output by more than 25 percent in certain parts of the world
June 23, 2017
Meet the Pratt Research Fellows
The Pratt Research Fellows are a distinguished group of engineering undergraduates who do extensive research in their engineering major during their final two years at Duke.
June 19, 2017
From Entrepreneurs to Encapsio
A Q&A with Three Duke Entrepreneurs Marrying Technology with Business
June 19, 2017
MEMS PhD Grad Selected for Women in Aerospace Symposium
PhD Grad Jing Li was one of 16 participants selected to present their research at the symposium.
June 16, 2017
From Durham to Silicon Valley: Duke Technology Scholars Program Helps Women Thrive in Tech
The 2017 class of 34 DTech scholars represents a next and accelerating step to increase the number of tech-savvy women working in computer science and engineering.


text:
As the pressure mounts to create strategies that deliver value, it has never been more important to evaluate your own portfolio and that of your competitors.
Wood Mackenzie's new Portfolio Tool allows you to use our world-class data to manage and optimise your portfolio across exploration, M&A and capital allocation all in one place. It includes statistical measures of asset and portfolio risk, so that you can make decisions based on a solid understanding of individual opportunities to maintain the strategic advantage.
Use the Portfolio tool to:
- Apply a probabilistic approach to valuation and risk using upside and downside cases
- Quantify risk at an individual asset and portfolio level
- Develop M&A strategies by varying the asset/corporate targets, timing of acquisition and level of equity acquired
- Model exploration campaigns by specifying forecast levels of drilling activity in targeted basins and plays
- Understand the key challenges in your portfolio around balance, growth and risk
- Manage capital allocation by delaying or accelerating new projects
You may also like
GEM. The valuation standard.
Exploration Service
Find out more
To discover more, register your interest below and we will contact you.


text:
Sometimes things just don't work right, and that's what your two favorite geeks are here for! Listen to them help solve your issues with batteries, email bcc, Photos migration and even CPU QoS. Download and enjoy!
MGG 612: The One Where Dave Kanye'd John
Sponsor: Casper makes an obsessively-engineered mattress at a shockingly fair price. Visit www.casper.com/mgg and get $50 towards any mattress purchase!
Sponsor: Mack Weldon will be the most comfortable underwear, socks, shirts, undershirts, hoodies and sweat pants that you will ever wear. Go to MackWeldon.com and get 20% off using promo code MGG.
Sponsor: TextExpander: The customizable typing shortcut utility – if you're not using it, you're wasting time.
Note: Show notes are complete!
Stuff mentioned:
- John's Classic 1994 Saturn SL2
- Removing/Cleaning the ignition coils/module on a Saturn S-series.
- Having Trouble Pasting Chrome URLs on a Mac? You're Not Alone and a Fix Is Coming. (7:30)
- Apple TV (Wikipedia some detail on pay and subscription options) (10:46)
- Sling TV Bundle
- Find my Friends (iOS App Store) (19:43)
- Podcasts (iOS App Store)
- ProcessRenicer (24:23)
- App Tamer
- TimeMachineEditor
- MailPluginManager
- DockStar
- OS X Server – Time Machine (43:28)
- Checking Very Large Time Machine Volumes (use RAM cache to speed up fsck_hfs)
- How to See What's Killing Your iPhone Battery (48:59)
- iOS: Updating your carrier settings
- Contacts Optimizer Pro (57:17)
- The Mac Geek Gab iPhone app
- You're downloading today's show from CacheFly's network
- BackBeat Media Podcast Network
- MacGeekGab AAC Enhanced Feed – Thanks to Michael Johnston of The iOS Show and Applr.


text:
by Waits
I write this morning because I feel like I finally can again. I cant get you out of my head. Your tenderness and the way you made me feel is one of the things I miss about you the most. When you died I died inside. I think of you constantly you probably consume 90% of my day as a lover should i guess. I have tried to imagine myself with someone else but every time a guy hits on me or seems interested I just start talking about you. I would feel guilty for ever being with someone else. You were the love of my life of eternity. My feelings are not going to change. I will not have anymore kids and yet I am young. I will not ever be in love again. Only you only you only you. Why cant people understand this? I am getting better but spending time at the cemetary seems peaceful for me. I just want to be somewhere you can hear me. Please give me a sign my love.. please
Comments for Lost Love
Click here to add your own comments
Tap into the compassion, support and wisdom of the
Free Stress


text:
$350.00 1 year ago
$350 Two dudes need a roommate
We are two college dudes getting a place near SFA- seeking a third guy near our age. We are 20 and 23. We are laid back and like to have a good time - our gfs will stay over sometime. Please include a little about yourself if you decide to reply. We would want to meet you beforehand
1 year ago
Nacogdoches, TX


text:
Today, I was pleased and surprised to receive the following news from the executive director, Mr. Laurie Woodruff:
Today is a red letter day for the Essential Civil War Curriculum as we published our 100th topic, Wade Hampton III by Bob Ackerman.While I realize that Google rankings are certainly not the primary indication of "worthiness" in any field, it is quite gratifying to see that ECWC is enjoying good results and broad appeal, and that I've been able to contribute in a small way. I encourage readers to use the site and read the essays. It is a great source to begin a study of the WBTS, as well as a great refresher and review for those who are already long term buffs and researchers. As the ECWC site description notes:
I thought you would like to know how the website is doing. September, a fairly typical month, saw 1,379 unique visitors who collectively viewed 29,087 pages on the website. This is a very good outcome for a still small and specialty focused website. More interestingly we are now beginning to show up high in the Google searches. For example if you Google George Pickett Lesley Gordon's material on the Essential Civil War Curriculum turns up on page 3. Considering the amount of information out there on Pickett this is a very good outcome. Google Civil War Artillery and Mike Martorelli's material on the Essential Civil War Curriculum turns up on page 2. And if you google Civil War Chaplains Richard Williams Essential Civil War Curriculum material is the fourth citation on page 1!
These Google results have not been managed through embedded search terms on the website but are occurring because of the frequency with which the website is visited and the quantity of material being added to it. Over the next month or so I will we working with the developers to add tools to the website which will raise the frequency with which we are found through searches . . .
Thank you in advance for helping us to move things along more quickly.
Bud, Jack, Paul and Laurie
Written and peer reviewed by today's foremost Civil War historians, the Essential Civil War Curriculum contains essays, bibliographies and other resources on the 400+ topics which constitute the basic knowledge that should be possessed by any serious student of the Civil War.For those interested, I've contributed the following two essays:
A Great Deal of Good: The Work and Impact of Chaplains During the American Civil War
And Men Will Tell Their Children: The Stonewall Brigade


text:
Mon, 3 October 2011
Recovering video game addict Tom Lennon (The State, Reno 911, I Love You, Man) tells The Indoor Kids about the horrifying struggles which led him to quit video gaming forever. Also, he shares his tips on getting laid. None of them, unfortunately, involve video games. :(
Direct download: Video_Game_Addiction_Episode_with_Tom_Lennon.mp3
Category:general -- posted at: 12:38am PDT


text:
You have Adblock enabled. Adblock has been known to cause issues with site functionality. If you experience any difficulties, please try disabling Adblock. Close
Ajouter à la liste de vos favoris:
Créer un nouveau dossier:
For man gay sex nude snapchat He spewed out that flow of steaming spunk
Recognize a pornstar in this video?
Help make pornstars easier to find on Tube8 by telling us who is in this video.
Thanks! The pornstar names will be added to this video when enough users submit the same names.
You must enter a pornstar name that already exists in our database.
- Catégorie: Twink
- Vues: 142
- Ajoutée: il y a 10 mois
- Description: For man gay sex nude snapchat He spewed out that flow of steaming spunk He spewed out that flow of steaming spunk in a bust of oh god's and oh's and he left a wet filthy goo fountain on his stomach when I was finished.
- Tags: gay, gaysex, gayporn, twink, twinks
- Stars De La X: Rajouter une star du X
- Favoris par: 0
- Signaler Cette Vidéo
Commentaires Vidéo
Commentaires sur toutes les vidéos (0)
Résultats de Vidéos Gay pour:
Sort by:
Sorry, we couldn't find any pages containing
Watch some of our most popular Tube8.com porn videos: Homepage or Category page


text:
Become a Sponsor - and enjoy the Benefits!
PCA sponsorships give your company the unique opportunity to build brand awareness with a concentrated audience of highly qualified professionals and decision makers in the Food & Beverage industry. Let us work with you to build a sponsorship package that meets your marketing objectives and your budget requirements. Opportunities start at just $5,000!
Place your brand in front of an audience that matters!
To become one of the PCA Sponsors please complete the form and simply send it back to us.


text:
|Publication number
|Publication type
|Application number
|Publication date
|Sep 1, 1981
|Filing date
|Jun 30, 1976
|Priority date
|Jun 30, 1976
|Publication number
|Alicia L. Compere, William L. Griffith
|Original Assignee
|The United States Of America As Represented By The United States Department Of Energy
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (9), Non-Patent Citations (1), Referenced by (16), Classifications (15)
|External Links: USPTO, USPTO Assignment, Espacenet
This invention was made in the course of, or under, a contract with the Energy Research and Development Administration. It relates generally to the art of immobilizing microorganisms.
Microorganisms vary greatly in their ability to attach themselves to solid material. Some microorganisms attach well to solids of biological origin. Some microorganisms even attach readily to artificial materials. Certain microorganisms form their own slimes upon solids to serve the purpose of producing growth sites. Most organisms, however, particularly the anaerobic microorganisms, have a rate of attachment to solids that is extremely slow and in some cases almost imperceptible.
In many systems it is highly desirable to have whole microorganisms attached to a support as opposed to the enzymes which the microorganisms produce. By so doing costly enzyme purification is avoided. Prior art techniques for achieving this result have immobilized microorganisms in an inactive or dead form. This technique is highly useful in many fields of endeavor. This method of attachment, however, tends to produce a support which has a lower enzyme activity than the live microorganisms. The use of inactive or dead microorganisms does not confront the problem of slow growth rate and attachment of live microorganisms.
It is thus an object of this invention to provide a process for immobilizing whole cell microorganisms.
It is a further object of the invention to provide a process for immobilizing whole cell microorganisms wherein the whole cells are alive and remain metabolically active with continued growth and reproduction functions.
It is a still further object of this invention to provide an article to which microorganisms will readily attach.
It is a still further object of this invention to provide such an article which promotes and encourages the rapid growth or culturing of the microorganism of interest when in the presence of appropriate nutrients.
These as well as other objects are accomplished by coating an aggregate support with a polyelectrolyte and crosslinking the polyelectrolyte to create a coating environment conducive to bacterial growth and life.
According to this invention it has been found that aggregate material may be treated by the process of this invention to produce a coating which is compatible with and conducive to anaerobic microorganism growth and existence. The technique for producing a growth stimulating and metabolically compatible coating comprises contacting conventional aggregate material with a polyelectrolyte and then crosslinking the polyelectrolyte by reacting it with a crosslinking agent.
Various shapes of aggregate material may be used in the process of this invention. These materials include conventional materials used for distillation and catalyst support reactions, such as pellets, stars, spiral rings, cross partition rings, raschig rings, berl and intalox saddles, pall rings, tellerette rings (flexirings), spheres, and irregular spheroids. The aggregate material can be composed of any conventional support material such as metals, ceramics, including both porcelain and stoneware; clay ceramics, including pottery clays; plastics, including polyethylene, polypropylene, and nylon; glass; woods, such as redwood; stones; cement and cement aggregates.
Live microorganisms can be attached to the crosslinked coating either during the crosslinking reaction or after. If attached during the crosslinking reaction, the microorganisms are locked within the crosslinked network. However, even with this locking in of the microorganism, the metabolic and growth activities of the microorganisms are not impaired. To the contrary, the growth and metabolic activity have been found to be surprisingly enhanced by the crosslinked coating environment. This advantage, however, is not limited to the coatings which have organisms attached during the crosslinking process but is present to the same extent in coatings which are crosslinked prior to contacting with the live microorganisms. Preferably, the crosslinked polyelectrolyte coating is selected so that it will have, across the pH range planned for use, a charge opposite to that found on the microorganisms across the same pH range. Where this is not possible, a second polyelectrolyte may be used either with the first polyelectrolyte or on the surface of the microorganisms, to provide a charge which is compatible with both the first polyelectrolyte and the microorganism.
The materials used in forming the coating of this invention are thus divided into a polyelectrolyte and a crosslinking agent. These materials form a coating on virtually any relatively inert material. The polyelectrolyte may be selected from the group consisting of gelatin, egg albumin, hide pulp collagen, refined collagen, fibrin, gluten and acrylic acid derivatives such as polyacrylamide and poly(hydroxy-ethyl methacrylate) and copolymers thereof. The polyelectrolyte may thus be crosslinked with a crosslinking agent to be insolubilized and to form a crosslinked coating. The crosslinking agent may be selected from the group consisting of glutaraldehyde, ethylchloroformate, formaldehyde, dimethyl adipimidate, N,N'-methylenebisacrylamide, 1,2-diacrylamide ethyleneglycol, N,N'-diallyltartardiamide, cyanogen bromide, concanavalin A, 6-aminohexanoic acid, 1-6-diaminohexane, and compounds having similar functional crosslinking groups but different chain lengths. The method of coating formation includes the preparation of a suspension or solution of the polyelectrolyte, together with such additional chemicals as may be deemed beneficial or necessary. The chemicals which are added to a polyelectrolyte suspension include chemicals, such as a secondary polyelectrolyte, which is added in order to give the solution or suspension desirable physical properties, such as a higher or lower viscosity; chemicals which are added in order to give the solution desirable chemical properties, such as a second polyelectrolyte which is added for the purpose of modifying the charge of the first polyelectrolyte in such a fashion as to produce a solution having an appropriate average charge; compounds which decrease the toxicity of the solution to the organisms being encapsulated, such as glycerol; small molecules, such as ferric chloride, which promote the mechanical strength of the crosslinked polyelectrolyte by providing cation or anion bridges between adjacent polymer strands; and the addition of chemicals, such as pennicillin and streptomycin, which inhibit the growth of undesired organisms in or on the hydrogel; i.e. the crosslinked coating.
The degree of crosslinking of the resulting coating is not critical. The higher the percentage of crosslinking the harder, of course, will be the coating. The degree of crosslinking can be somewhat ascertained by taking into consideration the fact that the site size should correspond to the size of the microorganisms which are generally within the range of 0.25 to 40 micrometers. As above stated the degree of crosslinking is not critical but a mixture comprising 10 volume percent polyelectrolyte and about 0.1 to 10 volume percent crosslinking agent is satisfactory for producing an article in accordance with this invention. Generally, it is preferred to have sufficient crosslinking agent to provide one carboxylic linking group from the crosslinking agent for every available amine group of the polyelectrolyte. About a 10 percent excess of crosslinking agent provides sufficient carboxylic acid linking groups to fulfill this condition.
The solution or suspension is then applied to the surface of the material to be coated using conventional methods, such as spraying, dipping, rolling, or painting. After application of the polyelectrolyte crosslinking is commenced. Under some conditions, crosslinking can be performed immediately prior to the application of the polyelectrolyte solution to the objects to be coated. In some cases, a stabilized form of polyelectrolyte, produced by, for example, microencapsulation, can be combined with a crosslinking agent, and an agent which releases the polyelectrolyte can be employed either at the time of application or immediately thereafter. Some polyelectrolytes, such as "Nalco" 8172, are supplied in a microencapsulated form to provide a decreased viscosity of the solution supplied.
A second polyelectrolyte, together with the microorganisms can be added and the crosslinking process repeated. If desired, both polyelectrolytes and the microorganisms can be added and crosslinked concurrently. Charged microorganisms coated with the second polyelectrolyte can be added to a solution circulating around the packing coated with the first polyelectrolyte after the first polyelectrolyte has been crosslinked. By virtue of charge, the second polyelectrolyte and the microorganisms will adhere to the crosslinked coating on the surface.
There are a variety of methods whereby microorganisms can be contacted for attachment to the packing material. One method comprises passing the microorganism suspension, in a liquid phase, through a bed of packing to which the crosslinked polyelectrolyte has been attached. This may be done by passing the liquid phase either up or down through the packed bed. Another method involves the deposition of the microorganism on the packed bed either through spraying or through other means of depositing small amounts of material evenly on the surface of the hydrogel. This may be done with the microorganisms either in a liquid suspension or a dry, finely divided form. During this process, the packing material and the hydrogel can be either wet or dry. The dry forms may be desirable where the coated packing material with the included microorganisms will be shipped or stored for a substantial period of time.
The major elements in the contacting of the microorganisms with the coating are the even coating of finely divided microorganisms either suspended in air or in a suitable liquid medium so that they arrive at the surface of the coating under conditions which promote the adherence of the microorganisms. Such conditions include provisions for appropriate physical considerations, such as temperature, and shear stress, for the adherence and growth of the microorganisms.
Having generally described the process and coating produced thereby embodied within the scope of this invention, the following specific examples are given as a further illustration thereof.
Glass squares, 15 mm on a side, were spray coated with different materials and combinations of materials. The materials included a 10% w/v solution of gelatin (Preiser); a 1% w/v solution of Nalco 8172 polyelectrolyte; and a 5% w/v solution of champagne yeast, with and without 1% added ferric chloride. The materials were sprayed in a thin layer onto the glass plates, and crosslinked with a spray of 5% w/v glutaraldehyde. The plates were then dried for a period of 24 hours at room temperature. In some cases, the yeasts used were not sprayed onto the coating but were added to the incubation mixture at a rate of 1 ml of a 5% w/v yeast solution per liter. Randomly labeled glucose-14 C was then added at a rate of 1 μCi/liter of medium.
The yeast plates were incubated in a medium containing 5% glucose, 0.5% tryptone, and 0.1% NaH2 PO4 w/v for periods of 1 and 4 days at 30 C. The plates were then removed from the medium and washed in 3 to 10 ml rinses of 5% w/v cold trichloroacetic acid. They were counted in a Packard Tricarb model 3375 scintillation counter in a scintillation fluid composed of 770 ml xylene, 770 ml p-dioxane, 460 ml absolute ethanol, 160 g napthalene, 10 g PPO (Packard), and 0.10 g POPOP (Packard). Yeast from the growth medium around the plates was centrifuged at 10,000 rpm for 10 min in a Sorvall SS-34 rotor. The supernatant medium was removed, and the yeast was suspended in 10 ml of cold 5% w/v trichloroacetic acid and filtered through a Whatman GF/C 45-mm filter. The filter was washed with two rinses of 10 ml of cold 5% w/v trichloroacetic acid. The filter was then counted as described above.
The fermentation of waste whey from the manufacture of cottage cheese to produce lactic acid was demonstrated using an anaerobic upflow packed bed bioreactor utilizing the coating of this invention. It was desirable to treat the whey, using some type of a continuous fermentation system, since there are a variety of problems involved in the storage and handling of large volumes of whey for extended periods of time.
A columnar bioreactor using gelatin-coated packing crosslinked with glutaraldehyde was prepared. The column unit was two inches in diameter by six feet high, and was packed with 1/4 inch berl saddles. The composition of the packing coating was produced using the same technique used for the glass square coating. A solution having the following constituents was prepared:
10% gelatin w/v
1% Nalco 8172 polyelectrolyte w/v
5% glutaraldehyde
balance water
Fifty milliliters of the solution was stirred with about one quart of packing material, 1/4 inch berl stoneware saddles, for a period of several hours until there was no glutaraldehyde odor. The packing was then dried. The packing was seeded with a mixed culture containing lactobacilli and lactose-fermenting yeasts derived from a commercial kefir culture. The unit was fed for several weeks on reconstituted dried sweet whey made to a concentration of 5% solids. It was then tested using a sour cottage cheese whey. The reactor was operated in an upflow mode at flow rates between four and eight liters per day. This value corresponds to a superficial retention period of 10 to 20 hours, although the actual retention period was lower due to the accumulation of biological solids in the reactor. Sample taps were provided every foot vertically so that changes in the whey column could be observed as it passed through the column. The product from the packed-bed reactor was passed through a bed containing 150 grams (dry weight) of Rohm and Haas IRA-94 macroporous weak anion exchange resin. The column containing the resin was a 2.5 cm by 100 cm column, allowing for nearly 100% bed expansion during washing. The fermented whey was passed through the resin bed until breakthrough occurred. When the excess whey was drained from the bed, and the resin bed was washed upflow with distilled water at a flow rate that gave nearly 100% bed expansion for a period of at least ten minutes. Then 200 ml of a 5% w/v solution of calcium oxide was passed through the resin, and the product recovered as calcium lactate. The column was then washed at 100% bed expansion with distilled water, rinsed briefly with a 1% NaCl solution, washed with distilled water, and placed on line again.
The product was crystallized by permitting the calcium lactate solution to evaporate at room temperature. The product recovered was a fine efflorescent white crystalline material.
Where it was desirable to recover the products from the ion exchange unit as acids, the unit was stripped with a strong acid, such as sulfuric acid, and the acid rinse together with a distilled water chaser, used for measurements of product recovery. Following strong-acid stripping, the resin can be regenerated in the manner described above.
Lactic acid was determined using a Varian 940 gas chromatograph having a flame ionization detector. The chromatograph was equipped with a 1/8 inch by 2 meter column filled with Porpak P and maintained at a temperature of 140 C. Nitrogen was used as a carrier gas, and hydrogen and breathing air were used to provide the flame.
Samples were prepared by mixing 1 ml of sample with 0.1 ml of H3 PO4. Standards, using reagent-grade lactic acid, were prepared in a similar fashion. Five microliters of the acid/sample mixture were injected into the column for lactic acid measurement.
There was a 1.4% lactic acid concentration in the sour whey which was used. This amount was increased after a single passage through the packed column, and reached a maximum of 2.1% after passage through the six-foot column. Following passage through the unit, the whey was stripped of its lactic acid using IRA-94 resin, which was produced by Rohm and Haas. The resin was stripped with 1 N sulfuric acid, and the lactic acid concentration in the acid-wash/water rinse was determined using a gas chromatograph. The lactic acid effluent from the resin was at a concentration of 1.7%. The acid rinse and water rinse had a volume of 1 liter. The total yield was 17 grams of lactic acid from 1300 ml of whey, which is equivalent to 62% recovery of lactic acid by the resin.
|Cited Patent
|Filing date
|Publication date
|Mar 7, 1963
|Mar 22, 1966
|Dow Chemical Co
|Removal of microorganisms from fluids
|Mar 19, 1970
|Mar 12, 1974
|Us Health Education & Welfare
|Insolubilized biologically active enzymes
|Oct 10, 1972
|Nov 19, 1974
|Penick & Ford Ltd
|Process of immobilizing enzymes
|Apr 4, 1973
|Jan 14, 1975
|Nat Patent Dev Corp
|Process of subjecting a microorganism susceptible material to a microorganism
|Feb 20, 1974
|Oct 28, 1975
|Denki Kagaku Kogyo Kk
|Immobilized enzymes
|Mar 27, 1974
|May 18, 1976
|Pfizer Inc.
|Immobilized microbial cells
|Feb 26, 1973
|Aug 3, 1976
|Research Corporation
|Preparation of protein membranes containing microbial cells
|Jul 1, 1974
|Feb 22, 1977
|Groupement D'interet Economique
|Continuous production of fermented liquids
|Feb 24, 1976
|Jun 28, 1977
|The United States Of America As Represented By The United States Energy Research & Development Administration
|Tapered bed bioreactor
|Atkinson et al., The Completely Mixed Microbial Film Fermenter, Trans. Instn. Chem. Engrs., vol. 50, 1972 (pp. 208-216).
|Citing Patent
|Filing date
|Publication date
|Oct 21, 1985
|Jun 7, 1988
|Owens-Corning Fiberglas Corporation
|Enzyme immobilization on non-porous glass fibers
|Apr 4, 1985
|Aug 29, 1989
|Verax Corporation
|Weighted collagen microsponge for immobilizing bioactive material
|Mar 17, 1987
|Nov 7, 1989
|Kimberly-Clark Corporation
|Multilayered structure containing immobilized blud-green algae
|Mar 17, 1987
|May 1, 1990
|Kimberly-Clark Corporation
|Immobilized blue-green algae
|Nov 13, 1989
|Sep 4, 1990
|The Standard Oil Company
|Production of polysaccharides from filamentous fungi
|Apr 15, 1988
|Feb 19, 1991
|Solohill Engineering, Inc.
|Collagen-coated polystyrene microcarrier beads
|Apr 5, 1989
|Mar 31, 1992
|Verax Corporation
|Weighted microsponge for immobilizing bioactive material
|Jul 2, 1990
|Aug 11, 1992
|Gist-Brocades N.V.
|Immobilized biocatalysts
|Jul 18, 1989
|Jan 5, 1993
|Kampen Willem H
|Process for manufacturing ethanol and for recovering glycerol, succinic acid, lactic acid, betaine, potassium sulfate, and free flowing distiller's dry grain and solubles or a solid fertilizer therefrom
|Feb 3, 1992
|May 24, 1994
|Preparation of novel immobilized biocatalysts
|Jun 14, 2013
|Dec 15, 2015
|Microvi Biotech, Inc.
|Biocatalyst compositions and processes for their use
|Dec 13, 2013
|Feb 9, 2016
|Microvi Biotech Inc.
|Bioconversion processes using water-insoluble liquids
|Dec 13, 2013
|May 10, 2016
|Microvi Biotech, Inc.
|Bioprocesses for making butanol
|Mar 4, 2004
|Sep 2, 2004
|Sheng-Ping Zhong
|Polycarbonate-polyurethane dispersions for thrombo-resistant coatings
|Oct 22, 1986
|May 7, 1987
|National Research Development Corporation
|Microcarrier for cell culture
|Dec 19, 1988
|Jun 29, 1989
|Willem Hemmo Kampen
|Process and apparatus for manufacturing ethanol, glycerol, succinic acid and free flowing distiller's dry grain and solubles
|U.S. Classification
|435/176, 435/180, 435/177
|International Classification
|Cooperative Classification
|European Classification


text:
"I'm not a selfish guy. Me and him play the same position, but if he ends up starting or not, I just want to see him perform every game," Black said after Memphis' 119-67 win against the Magicians Wednesday night at FedExForum.
Simpson, a 6-foot-10 junior college transfer, scored 14 points in 18 minutes and pulled down seven rebounds in his debut for the Tigers. He was active defensively, often altering shot attempts with his length.
He also showed off a smooth offensive repertoire, scoring the first two points of his Memphis career with a turnaround jumper on the block.
"It felt really good playing in front of Memphis for the first time," Simpson said.
The main knock on Simpson, who spent two years at Illinois before electing to go the junior college route, is his consistency. While Simpson is skilled offensively, he can disappear at times and his intensity tends to fluctuate throughout the course of a game.
Still, it was encouraging for Memphis coach Josh Pastner that Simpson more-than-adequately spelled a struggling Black, who had more turnovers (three) than points (two).
"I felt that Stan could help us if he played hard, which he did tonight," Pastner said. "He gave us some good minutes. We need that production out of him every night."
It would be a huge asset for Pastner and the Tigers, especially against the more talented teams the Tigers will face this year, if Simpson can consistently step in for Black, who showed last season that he can be foul-prone.
"I love that Stan did a real good job," Black said. "If he sees more minutes when the season breaks down, you never know what his stats will look like. That's what I like seeing, that I'll have a big man with me for another year that will help me."
Simpson's other teammates were impressed with his efficiency against the Magicians, especially considering Simpson missed the first week of team workouts in August because of a holdup with his summer school transcripts.
"He came in and adjusted perfectly," sophomore guard Antonio Barton said. "He was awesome. Blocked shots, rebounding. His low-post production — he can shoot it from beyond the paint and everything. Stan looked good tonight."
Simpson said he felt like he took the first step Wednesday night in solidifying his spot in the Tigers' rotation.
"I know I can get better and do better things to help the team out in future games," Simpson said. "It feels good to know that I'm a part of a good team and contributing right away."


text:
Stores »
A clever idea that keeps wax from dripping onto the cake. Great for kids; birthday parties! $6.95.
These look just like disposable plastic Solo cups, but they're actually made of melamine, so they're totally reusable (and dishwasher safe!). I love them! Perfect for outdoor family BBQs.
We are a community of shoppers sharing our best finds from across the web.
© 2017 ThisNext, Inc. ThisNext is a member of BUZZMEDIA Entertainment, a division of BUZZMEDIA.


text:
Essentials for the oyster lover.
The perfect gift for an oyster lover -- choose the glove and knife; the book; or the glove, knife, and book.
Shucked, Signed Copy: Food writer Erin Byers-Murray spent a year and a half studying the quirks and lifestyle of the oyster farmer, and documents it all in Shucked -- a captivating peek into the industry. Comes with a signed bookplate.
Oyster Glove and Knife: These are the gloves real oyster farmers wear for dragging, culling, and shucking. Protect yourself properly with your own pair -- and use this perfectly contoured oyster knife to get the job done.
Sourced from: Island Creek Oysters
Our return policy.
Photography by Rocky Luten and James Ransom.
Dustin Mortensen
$46 Exclusive! [More Styles]
And never need to look up the recipe again
Make Jamie Oliver's Crazy, Genius One-Pot Chicken
11 Cold Porridges For Hot Weather Breakfast
Ending Soon: Cookware Sale!
Why Back-to-Basics Cookbooks Reign
Seedlip: The Drink That's Gonna Make Your Summer
Get the recipes and features that have us talking, plus first dibs on events and limited-batch products.
(Oh, and $10 off your order of $50 or more in the Food52 Shop, too.)


text:
On Tue, Apr 2, 2013 at 12:03 PM, Jeff King <p...@peff.net> wrote: > > This message is duplicated, and is quite long. Let's factor > it out, which avoids the repetition and the long lines. It > will also make future patches easier as we tweak the > message. > > While we're at it, let's also mark it for translation. > > Signed-off-by: Jeff King <p...@peff.net>
LGTM! Signed-off-by: Garrett Cooper <yaneurab...@gmail.com> -- To unsubscribe from this list: send the line "unsubscribe git" in the body of a message to majord...@vger.kernel.org More majordomo info at http://vger.kernel.org/majordomo-info.html


text:
Tag: 1PONDO 073016_350
One the road private orgies – Yui Nishikawa
20.48K Views0 Comments10 Likes
JAV HD | YUI NISHIKAWA | JAV UNCENSORED Yui Nishikawa first appearance on a single path in 3P inside out. Yui foam cushion comfort rubbing a meat likely embrace the men. A large number of sperm Hobari two thick chi...


text:
Multiple Product Pages Versus One Product Page
Posted 30 June 2008 - 04:26 PM
I've noticed a competitor of ours has those models all lumped together on one product page, utilizing drop-down menus as options. I'm not sure, based on SERPs, if they are ranking better than we are. I would guess they are ranking better for general terms while we are ranking better for specific terms. But that's just a guess.
However, I would think that one page would be best, because then we would have all links going to that page (regardless of model), we could optimize the copy and product page instead of worrying about duplicate content penalties, and the keywords wouldn't be diluted because they show up in so many other places on the website.
What are your thoughts?
Posted 01 July 2008 - 08:30 AM
Assuming that we are talking about e-commerce site, from my experience one page is much better. Not only because of the link juice flow and rankings, but also from the user experience point of view. If you have 1000 products and each available in 20 variants, trying to make each option a separate product and page (20k) wouldn't be the greatest idea. Your goal is to make the user experience great, not to confuse them.
Having said that, I can imagine that in some cases (when competing against 'giants', with no hope to rank well for more general keywords) targeting long tail keywords with separate pages may be a good idea.
Before making any decisions I would analyse the amount of traffic you are getting to those pages (versus what could you be getting if ranking better for general keywords) and how do visitors convert.
Posted 01 July 2008 - 10:29 AM
I would pick a couple products that get a lot of traffic and convert those pages. See what happens to the conversion rate.
Questions like this in my opinion should not be answered by webmasters. Instead webmasters should present their possible answers to the site visitors and let the decision be made based upon performance.
Posted 01 July 2008 - 12:14 PM
Posted 06 July 2008 - 05:05 PM
Good Luck
Posted 06 July 2008 - 07:53 PM
I did a quick check on wordtracker for "chevy malibu" and there majority of searches are for the year followed by chevy malibu, e.g. 2002 chevy malibu
Same for "Honda civic" and the same for "jeep grand cherokee" although this one had a few extra phrases for parts, accessories and other options.
Posted 07 July 2008 - 02:25 AM
Posted 08 July 2008 - 06:51 PM
0 user(s) are reading this topic
0 members, 0 guests, 0 anonymous users


text: NCAA Afternoon Round-Up 4/03/12 By Rose Krzton-Presson • Apr 3, 2012 Robert L. Knudsen / wikimedia commons Today on NPR: The Obama administration has proposed big changes for the early childhood education program Head Start. Central Kentucky: A bill to help students from coal mining counties in Kentucky finish their education is once again in limbo. UK Celebrations Continue By Brenna Angel (KPR) • Apr 3, 2012 www.secsportsfan.com Thousands of UK fans packed into Rupp Arena this afternoon for a celebration of the men's basketball teaming winning the NCAA title. Think of it as a mini version of Big Blue Madness. More than 20,000 people got free tickets to the Kentucky celebration at Rupp Arena this afternoon. Robert Rose drove up from Powell County to get a look at the 2012 Wildcats and their championship trophy. "Means everything to me and my family. We've been UK fans, raised on it since we was little." Wildcat Fans Get Out of Hand By Associated Press • Apr 3, 2012 Associated Press Lexington police say they've arrested several dozen people following the Wildcats' 67-59 win over Kansas in last night's NCAA championship game. Fans filled the streets near the university campus within minutes of the game's conclusion. They jumped up and down, screamed, sprayed beer and waved Kentucky flags. Celebrations grew violent as one man was shot, though his injuries were not life-threatening. Afternoon Roundup 4-2-2012 By Chad Lampe • Apr 2, 2012 Chris Arnold NPR On NPR this afternoon: The Cutting Edge of Table Saws From WKMS: The Bridge: Kentucky Transportation Cabinet crews are working on the remaining spans of the Eggner's Ferry Bridge ahead of the arrival contractors to replace a collapsed portion of the bridge. Alcohol: Afternoon Round-Up 3/30/12 By Rose Krzton-Presson • Mar 30, 2012 www.kremlin.ru. / wikimedia commons Today on NPR: The Economic Impact Of Killing 'Pink Slime' Frankfort: The Kentucky Senate has passed an amended bill that would help the state repay interest on a federal unemployment insurance loan. Afternoon Round-Up 3/27/12 By Rose Krzton-Presson • Mar 27, 2012 John Karakatsanis / wikimedia commons On NPR: To keep customers, brick-and-mortar stores look to smartphones. Education: Kentucky lawmakers are trying again to raise the state's dropout age. Beshear and Abramson Discuss "Dream Game" on ESPN By Phillip M. Bailey (KPR) • Mar 27, 2012 Youtube Appearing on ESPN's Sportscenter, Governor Steve Beshear and Lt. Governor Jerry Abramson discussed the upcoming game between the Louisville Cardinals and Kentucky Wildcats in the Final Four of the NCAA tournament. The excitement over the archrivals playing for a spot in the championship game spilled over into the political arena earlier this week when Louisville Mayor Greg Fischer and Lexington Mayor Jim Gray made a friendly wager over Saturday's outcome. Mayors of Louisville, Lexington Make Friendly Wager on Final Four Game By Brenna Angel (KPR) • Mar 26, 2012 Britney McIntosh / UK Athletics Louisville Mayor Greg Fischer presented his Lexington counterpart with a bottle of Woodford Reserve Bourbon today as part of a friendly wager on who will win the Final Four matchup between U of L and UK. Fischer says Lexington Mayor Jim Gray needs to brace himself for a loss in New Orleans. "I think later this week you all need to be focused on starting to medicate yourself because there's going to be a huge disappointment in Catland come Saturday." Racers Finish Historic Run 31-2 By Seth Helton • Mar 19, 2012 The Murray State mens basketball team's historic season ended Saturday when the Racers lost to Marquette 62-53. Isaiah Canaan scored 16 points for Murray State. The Racers finished the season 31-2. Racers Tip-Off Against Colorado State Tomorrow in NCAA Men's Basketball Tournament By Shelly Baskin • Mar 14, 2012 The Murray State Racers start their 2012 NCAA men's basketball tournament run in Louisville tomorrow morning. They face off against the Mountain West Conference's Colorado State Rams. The Racers went 30- 1 for the season, earning them a 6 seed from the Tournament Selection Committee. While the seed is relatively high for a one loss team, Racers Coach Steve Prohm says he thinks the decision was based more on the venue than their reputation. He says, Pages« first ‹ previous 1 2 3 next › last »


text:
Anti-Bihari remarks: HC exempts Raj Thackeray`s appearance in court
The Delhi High Court on Friday exempted Maharashtra Navnirman Sena (MNS) chief Raj Thackeray from appearing before a trial court here in a case against him for his alleged anti-Bihari remarks.
New Delhi: The Delhi High Court on Friday exempted Maharashtra Navnirman Sena (MNS) chief Raj Thackeray from appearing before a trial court here in a case against him for his alleged anti-Bihari remarks.
Justice Veena Birbal also issued a notice and sought the response from Delhi government and on a plea of Thackeray who has sought quashing of the criminal complaint against him for making the alleged inflammatory remarks.
The court also directed the government to take "necessary steps" within seven days and tagged the matter with several other similar petitions moved by the MNS chief.
All the matters are scheduled to come up for hearing on April 30.
As per Thackeray`s plea, a criminal complaint was filed against him in Garwah, Jharkhand, in 2008 under sections 153A (promoting enmity between classes), 153B (assertions prejudicial to national integration), 295A (maliciously insulting religion of any class), 500 (defamation) and 504 (insult intended to provoke breach of peace) of the IPC.
The complaint was lodged against his alleged inflammatory speeches against people of Bihar and subsequently proceedings were initiated by a magisterial court in Garwah which had issued summons and thereafter non-bailable warrants against him.
Aggrieved by the same, he had moved the Supreme Court which had stayed the proceedings in the Jharkhand court and transferred the case to Delhi.
He has moved the high court seeking quashing of the criminal complaint in the trial court here as well as the summoning orders and non-bailable warrants issued by the Jharkhand court.
He has contended no sanction was sought prior to initiating of the criminal proceedings against him.


text:
Re: Info re: Pedro Benjamin Mendoza, 1927?
In reply to:
Info re: Pedro Benjamin Mendoza, 1927?
jessie turner 3/02/11
If you haven't already done so, I would suggest ordering a
copy of Pedro's Social Security Card Application. It will have
his place of birth in addition to his parents names. I'm
assuming you have a date and place of death already. And one more thing, who was he married to.


text:
Andy Murray became the first British man in 76 years to win a grand slam singles title on Monday, fighting off defending champion Novak Djokovic to win an epic U.S. Open final.
The Scotsman, beaten in his four previous grand slam finals, made it fifth time lucky with a nerve-jangling 7-6 7-5 2-6 3-6 6-2 victory at a windy Arthur Ashe Stadium where the players had to battle the elements as much as each other.
With both men struggling to control the ball in the gusting winds, and battling exhaustion in a slugfest that tied the record as the longest final at Flushing Meadows, Murray emerged triumphant, proving he belongs among the game's elite.
"I proved that I can win the grand slams," Murray said.
"And I proved that I can last four‑and‑a‑half hours and come out on top against one of the strongest guys physically that tennis had probably seen, especially on this surface.
"(I learned) to not doubt myself physically and mentally from now on. I'm sure that would have a positive impact in the future."
After losing the first two sets, Djokovic suddenly raised his game to win the next two and force a deciding fifth set, seizing the momentum as Murray started to wilt.
But as Murray's supporters, including actor Sean Connery and Manchester United manager Alex Ferguson, shifted uneasily in their seats, the Olympic champion regained his composure and jumped out to a 3-0 lead in the final set before hanging on to seal an emotional victory.