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Original Article Source(s): Air Nomads, Airbending, Council of Elders, Aang, Yangchen, Air Nomad Genocide
The world that Avatar: The Last Airbender ( A:TLA ) encompasses is vast and diverse, much like our world today. Within this, four main societies balance their universe: The Air Nomads, The Water Tribes, The Earth Kingdom and The Fire Nation. In their names alone we can see that each society is different and with these differences they bring not only balance, but harmony to their universe.
The specific topic for today is the Air Nomads who inhabit the vast Air Temples in the world of A:TLA. Unlike the other societies, they are spread throughout the world and do not occupy one place. Overall, they are governed by a theocracy based system and place utmost value upon life, viewing all things sacred. Thus, they are avid practicers of civil disobedience ( passive resistance ) and in general a society that advocates peace and acceptance. However, this also constitutes for their eventual genocide at the hands of Fire Lord Sozin.
From what we know, the Air Nomads ( as well as all other nations ) are quite controversial due to many questions posed by fans at the end of the season of A:TLA. Today, we the fans are going to put forth all these mysterious inquiries and together debate on their culture and derive possible concepts to satisfy our thirst of knowledge to the wonderful cartoon Nickelodeon has shared with us. This is not going to be a lesson of facts of the Air Nomads, as The Avatar Wiki already provides that for us, but rather a section of debate that we can finally discuss and share our opinions on what we think. I will put forth the many questions I have, and attempt to answer them. However, you the fans, as I am merely just a fan myself, may also pose questions and are welcome to add them as you wish. With that lets begin:
1) Why are the Air Temples spread throughout the world?
- I feel that the Air Temples are spread through out the world due to their great appreciation of life and high spiritual value they place upon their society. In order to completely understand a world, you must encompass it and thats what the Air Temples quite literally do: They have a location set in each major section of the world: North, South, East and West.
2) How does the society detail family?
- This concept has always baffled me about the Air Nomads. It would seem that they act as a family of whole, meaning that when a child is born they are not to distinguish a mother and father but rather view all his allies as his "brothers" and "sisters" and their parental figures as "Elders." I always found it peculiar that Aang considered Monk Gyatso his fatherly figure, but there is no evidence to his birthright parents, or even that he was orphaned ( Based on his birth scene shown in "The Avatar and The Fire Lord" ). This concept has always caused me to wonder on how they associate with their society, if marriage is practiced and overall how romance is handled. The creators themselves joked about this at ComicCon 2010 when they did the short "Forbidden Love" and had Aang admit the Monks weren't forthcoming on the reproduction process ( o.0; ).
3) Do you honestly believe that all Monks were indeed advocates of passive resistance, especially with the attitude and demeanor of Monk Tashi?
- I believe that Monk Tashi is a great example of the more trifling side of the Air Nomads society. Rather than take a more spiritual and humble approach to Aang being the Avatar, as Monk Gyatso did, Tashi was more than willing to put forth telling Aang his duty at an early age to prepare for an upcoming war. In this, it is obvious he sacrificed his cultural beliefs for a more needed purpose, but it also questioned if the Air Nomads, if given the opportunity, would have held their ground against Sozin had he not used an ambush tactic. As the High Monks were in agreement to send Aang to the Eastern Air Temple to complete his training, it may be the case that the Monks may have had a more aggressive nature like Tashi, and Gyatso was more kind in nature, which Aang cherished, and was the foundation of Aang's passive resistance attitude. In other words, Aang's evocation of the Air Nomads in reflection of Gyatso's position as one, and may not be entirely what the Air Nomads society is truly about since Aang is only a child, and not an adult. Thus, we may not truly know what their culture is about, and only Aang's opinion of what it is.
4)How do you perceive Avatar Yangchen's comment to Aang, "Many great and wise Air Nomads have detached themselves and achieved Spiritual Enlightenment."
- The conversation with Aang and Yangchen has always made me wonder even more about the Air Nomads. With that comment, she says spiritual enlightenment is obtained by detaching yourself from worldly things, much like how Guru Pathik says Aang much detach himself from the worldly objects that marry him to this world. Aang struggles in doing this because that would mean he has to sacrifice his love, Katara, in order to invoke the cosmic energy, thus becoming a fully realized Avatar. Correlating these two concepts, it would seem that the High Monks are not phased by the worldly attachments and may have no issue in eradicating them as their spiritual enlightenment teaches them of the greater, good things in life are beyond worldly concepts and that these things may be sacrificed in order to keep balance in the world. As Aang struggles doing this, and even goes as far as sealing his 7th chakra by choosing attachment, it would seem the Air Nomads philosophy is in conflict with his interpretation of the Air Nomads philosophy, meaning he may have not fully understood it and instead prioritized Gyatso's perception, which in a sense is skewed.
5)Airbending is a major part of the Air Nomads society. However, do you feel that Airbending is something the Air Nomads taught to their people through spiritual training or is something the individual is born with that defines them as an Air Nomad?
- The fact that bending acquisition is a debatable topic in itself, we must realize that bending in each nation is considered almost like a race, thus beliefs and practices differ in each case. With that in mind, the Air Nomads were very unique in the matter that each member possessed the skill based on the knowledge and scenes we are given in the cartoon. With that, they seem to correlate spiritual strength into their bending capabilities. Thus, it seems to be that though the Airbending is found in all the Air Nomads themselves they place a barrier of spiritual acquisition in order to use it effectively. As each major element is in-tuned with certain aspects of human emotion, it seems "Faith" may be the crutch the Air Nomads lean upon. That in itself may support that the reasons why there is an abundance of Airbenders within the Air Nomads, because of the spiritual intuition of the parents may be generating their child to be bestowed with the gift through the pregnancy and birth process. In other words, their spirituality is strong enough to influence the genetic processing, much like spirituality of Chief Arnook bestowing Princess Yue with the genetic make-up to revert her illness through the recognition of Tui.
6)Given that the Air Nomads were ambushed with the element of surprise, had they not been do you think that Fire Lord Sozin would have been able to successfully carry out the Air Nomads genocide?
- Aang tells us during "The Headband" that Fire Lord Sozin won by ambush. However, he merely states that the Air Nomads had no formal army, meaning that they may have had some defense procedures in place. With this, and the acknowledgement that the Air Nomads were aware of the upcoming war makes me wonder how the battle would have turned out. The one fact that I always pondered was the Air Nomads were aware of Aang's disappearance so they, as it obviously states, must have prepared for the onslaught. Also, as there were many casualties, shown in "The Southern Air Temple," meaning they were able to put up a decent battle. There are many factors that support each side. The Fire Nation was enhanced by Sozin's Comet, but did not have the mechanical technology they had in the era of the series. The Air Nomads had a debatable ethic of war but an obvious obligation to participate, but did have their trump card: The Avatar. I, personally, feel that Sozin would have been victorious because of the Fire Nation's enhanced ability being their trump card and its availability to them. However, the Airbending techniques being executed with an abundance of benders may also have been equally as devastating as Airbending is defined as being the most evasive and dynamic, something heavily in contrast to Firebending. So tell me, who do you think would have won?
7)What do you, fellow users and debaters, want to put forth about the Air Nomads? What do you want to share and know?
- Please users, I want to hear your questions and responses. I have put forth my interests, and now I want to hear yours! YES MS. KWAN! MY MIND IS READY TO BE MOLDED! But... By you, the user!
- Please remember, this is my opinion and I want to hear your opinions! Don't be shy, but please don't focus on correcting every little mistake. Instead, use your examples to prove your points rather than degrading another user. This way, the conversational element of this topic remains true and others won't shy away voicing their opinion.
- I deliberately did not include many elements so other users can have a chance to voice their opinions. Remember, this blog isn't just so I can get what is on my mind heard, but rather so you can be heard to and we all can get feedback! :)
- I will provide 4 comments that will be titled as each question I have put forth that can be used as mediums to discuss and debate what I have put forth.
- My 4 questions should not be the only thing discussed. If you feel you have something you want to contribute and put forth, PLEASE DO! Start a new comment with your question, then reply to it with your detailed explanation ( I suggest doing the question and explanation on a separate text document and then copy and paste it so you don't run into too many people responding before you get a chance to explain your stand point ).
- Please be respectful to yourself and users by not destroying the integrity of the blog. After all, it is the tradition of Air Nomads. Or is it?
Prince Azula 20:53, October 4, 2010 (UTC)


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U.S. Senator Chris Coons (D-Del.), a member of the Senate Judiciary Committee, voiced his strong support for the bipartisan legislation introduced Wednesday to reauthorize the Violence Against Women Act (VAWA), which has enhanced law enforcement's ability to investigate and prosecute violent crimes against women since its original passage in 1994.
"For nearly 18 years, the Violence Against Women Act has shined a national spotlight on a problem that was once considered a private matter, empowering women and men alike to take a stand against abuse and increase the safety of countless women across America," Senator Coons said. "By reauthorizing this legislation, we will continue the fight first championed by Joe Biden, while enhancing initiatives that confront the evolving needs of the push to root-out domestic violence. Without Congressional action, many of VAWA's programs will expire this year. As a matter of human rights, we simply cannot allow that to happen."
VAWA was first signed into law by President Clinton in 1994, and has since been reauthorized in 2000 and 2005. The law's current authorization, however, expired in September. Programs supported by VAWA have provided victims with critical services such as transitional housing, legal assistance, and supervised visitation services. VAWA has also encouraged communities to coordinate their responses to domestic and sexual violence by bringing together victim advocates, law enforcement, the courts, health care professionals and leaders within faith communities.
"As state and local budgets struggle to find the resources needed to fund enforcement of domestic violence laws, as well as for aid, relocation and support for victims, VAWA provides an important lifeline to some of our most vulnerable citizens," Senator Coons said. "We cannot afford to let these programs lapse. I will continue to advocate for reauthorization of this legislation and encourage my Senate colleagues to swiftly pass this bill."
The legislation introduced Wednesday would improve existing programs to address evolving needs in the fight against domestic violence, dating violence, sexual assault, and stalking. The proposed legislation will provide tools to prevent domestic violence homicides by supporting training to those law enforcement officers, victim service providers and court personnel who are working on the front lines to eliminate domestic violence.
Importantly, the legislation also promotes accountability to ensure that federal funds are used for their intended purposes, and consolidates programs and reduces authorization levels to address fiscal concerns while focusing on the programs that have been the most successful.
The legislation was sponsored by Senators Patrick Leahy (D-Vt.) and Mike Crapo (R-Idaho).
Source: http://coons.senate.gov/newsroom/releases/release/senator-coons-calls-for-reauthorization-of-the-violence-against-women-act

subset: commoncrawl


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Main point of my project was to design a future concept of a sanitary set which is user friendly and where people don't have to make unnecessary movements. I have designed a sanitary set which is composed from moduls, which customers are enabled to combine and create a sanitary equipment which fits their needs. In my concept I use materials, which are environment friendly. The material which is mainly used in my concept is filled polylactic composite, which is biologically degradable. Design follows function - every angle and every shape has got a functional purpose ( for example angled mirror part create view from below, which helps for shaving etc.) Minimalistic design related to the pure culture of hygiene. This minimalistic approach is accented by cyan parts (symbolizing water), which begin to reveal to you when you start your interaction with the sanitary set. These perforated parts are in relation to wet environment of bathroom, decrease weight of removable parts and also emotionally divide the compactness of the product.
General Director's Prize of the National Technical Museum in Prague
Director's prize of the Institute of Art and Design at the West Bohemia University in Pilsen
Thank You!


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She is the latest product of the It-Girl factory: Veronika Heilbrunner. The German co-founder and editor of hey woman! is currently one of the most standing out girls in fashion industry. No matter if fashion magazines, Instagram or street style blogs: as a major source of styling inspiration she is everywhere!
A little bit of a mixture of Alexa Chung and Leandra Medine, but definitely with her very own taste of styling: There is nothing that we don't love about Veronika Heilbrunner. Together with her boyfriend Justin O'Shea, buying director at My Theresa, they are one of fashion's most photographed couples of the moment.
The basic pieces of a Veronika's wardrobe? Denims, cropped culottes preferred, and flat mountaineer style lace-up boots (but never together!). An other must upon a Veronika Heilbrunner inspired outfit is a print T-Shirt. The way she styles and combines pieces always turns out great – you could not tell if she was about to attend a fashion show or going for grocery shopping. Her looks work for the most different occasions. They are always stylish and dressed up, but in a casual way and not at all too put-together.
Especially for you, we picked three of Veronika Heilbrunner's most inspirational outfits and re-created them!
Look #1
SANDRO Midi Skirt - GANNI T-Shirt - GANT Boots
Look #2
HUGO Turtleneck - ZOE KARSSEN T-Shirt - SMH Lace-Up Flats - GUCCI Dionysus GG Supreme Bag - H&M Denims
Look #3
GUESS Belt - DELPOZO Blouse - M MISSONI Skirt - BURLINGTON Socks - MARC O'POLO Slippers
Images via Pinterest, Style du Monde, Vestiaire Collective


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" Whatever the truth, Wozniak later insisted that it was not worth rehashing.Boots Ugg Style The dark tan is hard to match things with.Ugg Tasman "We need it to have a classic look that won't go out of style, like the Volkswagen Beetle," Jobs said. An independent developer came up with the first spreadsheet and personal finance program for personal computers, VisiCalc, and for a while it was available only on the Apple II, turning the computer into something that businesses and families could justify buying. "It was a four-bedroom house, and we occasionally rented one of the bedrooms out to all sorts of crazy people, including a stripper for a while," recalled Jobs. Lackey on February 26, 2014 Color Name: CaribouSize Name: XX-Large Verified Purchase Jacket itself is great - good quality materials and workmanship. Intel's chips ended up becoming the industry standard, which would haunt Apple when its computers were incompatible with it. ugg boots uk cheap "That isn't the wind.Ugg Cove Boots Lackey on February 26, 2014 Color Name: CaribouSize Name: XX-Large Verified Purchase Jacket itself is great - good quality materials and workmanship."39 In February 2008, the Princeton Animal Welfare Society staged a campus protest against the fur industry, particularly attacking the ugg boot industry. One even had its distinctive Ugg trademark glued on to its heel upside down. The only outsider who saw this first Apple II was the hotel's technician. Wow, oooh, my parents were actually once very young and really good-looking.Ugg Boots CostBlack Ugg Shoes After work each day, he would change into his dungarees and retreat to the garage, often with Steve tagging along.
He was mercurial, sure of himself, a little dictatorial. Boots Ugg Style "I started pointing them out everywhere until he was completely convinced.Ugg Boot Sale Uk The only outsider who saw this first Apple II was the hotel's technician. Here is what I found: - UGGs are made of sheepskin inside and out, and cost $130-$160 for the most popular type (called Classic, short or tall), more for the "fancier" varieties (but I honestly prefer the look of the Classic model) - EMUs have TWO types of products: (1) sheepskin inside and out, and (2) cowhide outside, merino wool inside. "I thought my dad's sense of design was pretty good," he said, "because he knew how to build anything. For a minute I thought you might be a dream too. [Boots Ugg Style] At Atari his behavior had caused him to be banished to the night shift, but at Apple that was not possible.
Raskin was told to take a leave of absence.Ugg Boots Cost Mary thought of something all at once. I would appreciate letters from anyone who wants to pay up. Out of their slick packaging, the boots were clearly not made of Ugg's comfy sheepskin, but a cheap man-made fur." When members of the Mac team got ensnared in his reality distortion field, they were almost hypnotized. [Boots Ugg Style] Moore had tried to instill in the Homebrew an ethos of swapping and sharing rather than commerce.
Was it real? Terrell confirmed that it was, and the store agreed to front Jobs the parts on thirty-day credit. ugg boots uk cheap "How old are you?" he asked.[29] By the mid-1970s, several surf shops in Santa Cruz, California and the San Fernando Valley were selling a limited number of ugg boots purchased by the shops' owners while visiting surfing events in Australia." His guiding principle was "Form follows emotion," a play on the familiar maxim that form follows function. [ugg boots uk cheap] "I had read about DNA testing, and I was happy to do it to get things settled," he said.


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As soon as the weather gets cold, I start making atole, which is one of the many things I learned from my mom and my grandma. I even remember that at my grandma's house they used to make some black bean atole, and even though I tried it, I never really liked it. Black bean atole? Go figure!
- 6 tablespoons of corn masa harina
- 2 cups of water
- 2 cups of orange juice*, with seeds removed.
- 4 tablespoons of sugar


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Mixing engineer from venezuela, owner of UltraSonido Recording Studios
Audio engineer and music producer working with many bands and projects in venezuela and other latin american countries the last 8 years.
Tell me about your project and how I can help, through the 'Contact' button above.
My credits include
Gear highlights
- Classics LA-3A
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Terms of Service
allow typically 2 revisions, 4 days for mix
Reviews of Roberto Garcia
Roberto was completely professional. He delivered on time. Amazing ear.


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Aetna Foundation Prize for Health Equity Innovation
With the support of the Aetna Foundation, InnovateHealth Yale offers funding to student teams focused on reducing health disparities in the United States.
Aetna Foundation Prize for Health Equity Innovation ($25,000):
The Aetna Foundation Prize for Health Equity Innovation is a $25,000 cash prize awarded to the best student-led venture focused on developing a technology solution to address a health disparity challenge. Examples of challenges include higher rates of health conditions, worse health outcomes and less access to care. Solutions which involve mobile technology are actively encouraged, but the prize is open to any solution involving a technological solution. The winner of the Aetna Prize receives legal services from Whithers Worldwide, consulting from Make Good, preferential consideration for the Yale Entrepreneurial Institute Fellowship (a summer program that provides a $15,000 stipend), and continued support from Yale faculty.
To apply for the Aetna Foundation Prize for Health Equity Innovation, please see key dates and links below:
Mentorship Application (optional)– due Jan 30th
- To help you apply for and win 25k, we will match you with a mentor/industry expert who will work with you during the Spring semester. This application is optional, but we strongly encourage you to take advantage of this resource if you are planning to apply to the prize.
- Full Application (applications open Feb 1st) – due March 30th
- Final pitches – April 21st
Aetna Foundation Prize for Health Equity Innovation will be part of StartUp Yale 17 on April 21st, 2017. Startup Yale brings together Yale's biggest entrepreneurship awards in one day of pitches, prizes and world-changing ideas. For more details and to register for the event click here.


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The Australian SAS: the untold history reveals the secretive operations and unorthodox military culture that make the regiment unique.
Where other Special Forces documentaries portray the superficial veneer of soldiers in fast paced action, The Australian SAS: the untold history goes beyond the media clichés and Special Forces action stereotypes. The series reveals the real people, and the creative and strategic flair in war and peace that have won the Australian Special Air Service Regiment – "SAS" – its place in the top Special Forces units in the world.
Part 2 - Arc of Instability throws SAS into the fearsome jungles of Borneo. As Konfrontasi rages in Indonesia, SAS joins with Britain's 22 SAS in the highly secret Claret Operations. Cross border patrols gather strategic intelligence and seek out Indonesian 'Killer patrols'. In 1966, when the European Cold War spreads to Asia, SAS joins the Australian Task Force in Vietnam. Small secretive five man SAS patrols set out to pit themselves in a deadly anti-guerrilla war against the fearless & formidable Viet Cong.
Exempt From Classification, NTSC, all regions. 2 DVD set. Disc 1 - 26 mins plus photo gallery. Disc 2 - Extra interviews.


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This page is only kept for historical reference
The original idea of the HowTo was to be a site providing public information on how to engineer things in natural environment from scratch, based on the idea on haflbakery.org:
A site providing public information on how to engineer things in natural environment from scratch
Humans were able to create cars, planes, computers, rockets, sattelites etc. By doing so, they proved that all these things and more CAN be made only using human power, without any special instruments at the beginning. This suggests an idea that with sufficient amount of time, it would be possible to create all these things using "bare hands" of a human beings. However many of the beings nowadays wouldn't have an idea how to engineer, for example, a computer, if they were asked to do it in the natural environment.
The idea is to create a website, which did provid such information.
Just like we have free software, we may have free technologies, which would help the developing world.
Finaly this may also produce real value, like Wikipedia already does. As said best by Alvin Toffler in late 60's (1969): information can substitute most of the material resources and becomes the main material for workers. As a result, information is the center of the creation of value and power, like capital/money were for a few years during the industrial age and like slavery/submission were for many more years (before the industrial age).


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Lacrosse news straight to your inbox.
Tuesday August 6th, 2013 1:00am
AMHERST, Mass. - The #8/15 UMass women's lacrosse team defeated Canisius 13-12 in the NCAA play-in game on Sunday at McGuirk Alumni Stadium, giving them an automatic berth to the NCAA tournament. A Jesse O'Donnell free position goal with four seconds left in regulation would give the Minutewomen the win.
UMass improves to a 19-1 record, just one win shy of tying the program record for wins in a season, and Canisius falls to 10-9 on the season.
Tanner Guarino and Jesse O'Donnell each scored four goals a piece in the victory, Guarino going a perfect three-for-three in free position goals. Lauren Terracciano notched two goals to go along with an assist and Dayna Defilese and Katie Ferris each put one in for UMass.
Canisius was led by Morgan Leonard and Megan Oosting, who each had three goals a piece, Oosting also assisted two times in the game. Maria Kotas was also a difference maker in the game, winning a team-high three draw controls and scoring two goals. She also caused a game-high four turnovers.
Katie Florence made seven saves to improve her record to 19-1 while Kayla Scully made 10 saves for the Golden Griffs.
UMass started things off, scoring the first goal of the game. Canisius fired back quickly however, scoring two in a row to take the lead but less than ten seconds went by before UMass answered to tie the score at 2-2. After the score tied again at 3-3, UMass went on a three goal run in 1:40 of play but were unable to keep their lead as the Golden Griffs came firing back with three straight goals of their own. The score would remain tied going into halftime with each squad scoring seven goals.
UMass scored first and early in the second half but were unable to break away from Canisius, as they continued to match any scoring efforts the Minutewomen put together. A falling down goal in the middle of the second half by Ferris gave UMass a two goal advantage with more than 15 minutes of play left. Canisius cut that lead to one exactly three minutes later and then would score again with 25 seconds left to tie the game at 12-12. O'Donnell won the draw for UMass and was fouled with four seconds left on the clock when she would be awarded a free position opportunity, which she capitalized on and gave UMass the victory.
UMass out-shot Canisius 28-20 but the two teams were even on ground balls with 11 apiece. The difference maker was draw controls, with UMass winning 19 and Canisius winning 8. UMass also never failed a clear attempt throughout the game, going a perfect ten of ten.


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|Elder Faming
|Voice Actor:
Takkō Ishimori
|Real name:
Fǎmíng Bǐqiū
|Chinese name:
|Also known as:
Abbot Faming
Bhikkhu Faming
Monk Faming
Priest Faming
|Divine name(s):
Elder Faming (法明長老, onyomi: Hōmei Chōrō) is a secondary character in Saiyuki: Journey West.
Role in GameEdit
Homei is the head monk of Gold Temple who found and raised Sanzo. With nothing to identify the child's origins aside from a violet blanket, he fondly named the infant Chin Genjo. When a sixteen-year old Genjo reports having a dream of Lady Kannon giving him/her a divine staff, Homei's disciples mock the claims and ignore him/her. Homei believes the dream when he inspects the staff personally. Granting the monk new robes and two escorts, Homei renames Genjo as Sanzo before seeing his disciple off.
After Heaven is restored, Lady Kannon and Sanzo appear before a surprised Homei. His temple is given the Holy Book of Dharma as a reward for their pilgrim's efforts.
Benign and wise, Homei genuinely cares for Sanzo. He is aware of the bullying the child experiences and seeks to cheer him/her up with sage advice.
Journey to the WestEdit
Faming was the abbot of Jinshan Temple who found his path towards true enlightenment. Introduced during the start of Xuanzang's backstory, he found the newborn son in a basket drifting along a river. On top of the baby's chest was a letter written by the boy's mother in blood, detailing the boy's family history. Tucking the letter away, he decided to raise the newborn child and named him River Current (Jiangliu).
When Jiangliu was seventeen years old, Faming allowed the young man to become a monk and granted him the Buddhist name, Xuanzang. After suffering harsh treatment for not knowing his past, a crying Xuanzang came to Faming. Deciding it was time, Faming handed the letter written by Xuanzang's mother to the youth. He helped Xuanzang reunite with his family at Jingzhou, even offering the temple as hospitality.


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TRAFFORD Council and Greater Manchester Police (GMP) have officially opened their pioneering new HR and payroll shared service Centre.
The organisations say the Greater Manchester Shared Services (GMSS) has enabled the two organisations to cut across traditional boundaries in order to significantly streamline and rationalise their HR support services and processes.
Based at Trafford Town Hall, the GMSS service brings together a team of HR, payroll and pensions experts from the two organisations. The team cater for more than 20,000 employees, including local authority officers, police officers and staff, specials and volunteers, teachers and education staff, and some NHS employees.
The HR service features a new online customer contact system and telephone based contact centre that increases the convenience, flexibility and speed in which queries are submitted recorded, tracked and dealt with.
The organisations say the service, which is currently dealing with more than 300 telephone calls a day and responsible for a combined payroll figure of £500 million, is already providing a cost effective and efficient HR service for both Trafford Council and GMP..
Announcing the launch of the service, Cllr Sean Anstee, Leader of Trafford Council, said: "We are extremely proud of our ground-breaking new shared HR and payroll service. Working in partnership with Greater Manchester Police has already brought about significant financial and tangible benefits for the two organisations.
"Trafford Council is acknowledged as one that is not afraid to do things differently – to take brave decisions and pioneer new ways of working."
"We believe our new service centre provides us with an opportunity to explore our ambitions of delivering services to other public sector partners and agencies, with the potential of developing a fully independent Public Service Partnership model."
Interim Greater Manchester Mayor and Police and Crime Commissioner, Tony Lloyd, said: "As part of the reform of public services, agencies across Greater Manchester are embracing new ways of working. This new service is an example of how we can effectively pool resources to provide a better service to our staff and the public.
"It is a common sense move, highlighting how far Greater Manchester has come in building a strong foundation of working together."


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The Cincinnati Reds have reached agreement with arbitration-eligible outfielder Adam Dunn on a two-year contract extension that includes a club option for the 2008 season.
Newly installed general manager Wayne Krivsky acted quickly to lock up the Reds' best power hitter for the next three seasons. Dunn, who earned $4.6 million last season, had sought $8.95 million in arbitration, with the Reds offering $7.1 million. Dunn's new contract will pay him $7.5 million in 2006 and $10.5 million in 2007. The club option for 2008 would keep Dunn off the market during his first year of free agency for $13 million.


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How to Make a high protein shake
The secret the fitness food companies don't want you to know !!! A guide of how to make a high protein milk shake with 33g of protein per pint for only 88p per pint!!!! Great for building up muscle fast and healthily !! Amazing


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|Message view
|« Date » · « Thread »
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|Steven Bethard <steven.beth...@gmail.com>
|Re: Parameters in uima descriptors
|Fri, 05 Jun 2009 05:44:21 GMT
On Thu, Jun 4, 2009 at 7:56 AM, Chris Roeder <email@example.com> wrote: > If you ship xml descriptors, then someone who creates CPEs with the gui > and runs them with SimpleRunCPE can use them easily. If you don't, > your components are still usable, though with a bit more work. Either > the user writes XML descriptors for them and proceeds with the CPE editor > and SimpleRunCPE, or they modify SimpleRunCPE or similar code (as I > understand you to have done ) to create the components in java without > getting the meta data from xml. Yep, I think this is a fair description. So I can certainly agree that not providing XML descriptors would make it harder to use SimpleRunCPE (and CPE descriptors in general). I'm uncertain as to how much of a loss this is. In our case at least, getting away from the CPE gui has been a blessing, not a curse. ;-) On Thu, Jun 4, 2009 at 8:26 AM, Thilo Goetz <email@example.com> wrote: > The most common way to reuse someone elses component is to embed > said component in your own UIMA processing pipeline. This is done > by referencing the component's XML descriptor in your own aggregate > descriptor. No XML descriptor, no referencing, no reuse. That's > what I mean by "unusable". Does that make sense? You mean "not usable in an XML descriptor". Yep, that's true. However, the code is still usable in a Java descriptor. See for example the various createAggregateAnalysisEngine() methods in org.uutuc.factory.AnalysisEngineFactory: http://code.google.com/p/uutuc/source/browse/trunk/uutuc/src/main/java/org/uutuc/factory/AnalysisEngineFactory.java Note that if you use something like the above, you can happily aggregate both Java and XML descriptors. But yes, you do have to commit to writing your main methods in Java instead of relying on something like SimpleRunCPE or the CPE gui. I do think that saying they're "unusable" is misleading though. If you want to say they're "unusable by XML descriptors" though, I'd be happy to agree to that. I'm just of the opinion that moving from XML descriptors to Java descriptors is a good idea, particularly for code which undergoes relatively frequent refactoring. Steve -- Where did you get the preposterous hypothesis? Did Steve tell you that? --- The Hiphopopotamus
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Cream Cheese Frosting Help
Decorating By CupcakesByIrene Updated 8 Sep 2013 , 2:53am by MBalaska
Hey guys i was wondering if anyone has a pipeable cream cheese frosting that can also be left out? I am making a red velvet cake and want to fill the cake with the frosting and pipe ruffles all around the cake. Any suggestions??
I know of this recipe:
I make one with even less cream cheese than that to agree to my state's laws and supplement the cream cheese flavor with Lorann Oil's "cheesecake" extract.
CucakesByIrene: here's a couple of cupcakes made with a normal butter/cream cheese frosting. Piping is ok for a top, buy I have never ever tried to put anything on the side of a cake with it, I don't plan to. They held their shapes for a week before we ate them all (out on the counter as I've never refrigerated them.) (Hobby baker here in chilly Alaska)
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Serenia is a location from the 1985 animated series She-Ra: Princess of Power.
She-Ra: Princess of Power
Serenia is a very mystical, powerful and beautiful place. It is the one place which keeps all of Etheria in balance. If anything were to happen to it, Etheria would tip sideways. It houses the Balance Center of Etheria as well as Candor's Mountain.
Madame Razz used to live in Serenia about 600 years prior to her time with the Great Rebellion


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Luzerne County acting Manager C. David Pedri has re-advertised the operational services division head position at a higher salary without a past requirement for applicants to hold a Pennsylvania professional engineer license.
Pedri stressed Thursday he has not ruled out the three finalists he already interviewed but is attempting to widen the applicant pool.
"We want to ensure we have reviewed the broadest range of applicants possible," Pedri said.
Initially $70,000 to $85,000, the salary range is $80,000 to $90,000 in the new ad posted on the open positions section of the county website, www.luzernecounty.org.
Applicants previously were required to be eligible for a Pennsylvania professional engineer license and obtain a license within one year of appointment. Licensing requires passage of an engineering fundamentals test, apprenticeship under a licensed professional engineer and completion of a lengthy exam, engineers say.
Pedri said he does not believe an engineer license is necessary for the management post because the division head is primarily responsible for the oversight of facilities and employees, "as opposed to engineering duties."
The operational services division head oversees engineering, roads and bridges, planning and zoning, 911, emergency management, building and grounds and security in addition to any county environmental or recreation projects.
The county's home rule charter and council-adopted administrative code don't require an engineer license for the position, Pedri said. The council's personnel code says the administration — not the council — sets qualifications for vacant positions.
The county already has a licensed engineer — Larry Plesh — on staff and access to the expertise of other licensed engineers who handle county projects as independent contractors, Pedri said.
The first two operational division heads under the county's home rule structure met the license requirement.
Tanis Manseau, who received $85,000 in the post, was a licensed engineer in South Carolina when he was hired in May 2014 and received his Pennsylvania license in December that year, state records show. Manseau resigned in October.
Christopher Belleman, who was paid $75,000, already held a license when he was hired as operational division head in July 2013. He resigned as division head in September 2014 to become county Flood Protection Authority executive director.
Several years before home rule, the county had three licensed engineers on staff — Belleman, Jim Brozena and Joe Gibbons. Gibbons and Brozena, who also served as flood authority overseer before Belleman, left county government to pursue other work opportunities.
A majority of the 11-member county council must confirm the acting manager's division head nominees before they take effect.
County Councilman Rick Williams, a state-licensed architect, said he has no problem with the new advertisement because someone without an engineer license may have the knowledge and skills to oversee the division.
"I support providing the administration with flexibility to find the best people to fill positions," Williams said.
The first review of operational division head applicants is March 30, according to the new job posting.
Pedri said he expects to present a division head nominee to the council in early April.
The county is accepting applications for two other division head posts.
Human Services Division Head David Schwille is retiring April 7, and J. Allen Nesbitt recently resigned as correctional services division head.
The advertised salary range is $80,000 to $90,000 for both positions, and the first review of applicants also is March 30.
Reach Jennifer Learn-Andes at 570-991-6388 or on Twitter @TLJenLearnAndes.


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Most practitioners in the therapeutic study are immersed in considerations other than punter/patient consideration for furthermost of their in employment hours. Healthcare in these United States is a business concern. Beyond the necessity of determination a underpinning to bestow your employment and next attracting grouping to come with to use them are the day-to-day considerations of management, plus exploitable in the structures and limitations of your own modality.
Often, by the occurrence you in actual fact get to sit downfield near somebody junction to you for help, your person in charge is so flooded of the point of the day; the mechanised fairly than the in the flesh. It sometimes takes a colossal act of will to get in progress near different quality self. Becoming latter-day is the variety of piece that an act of will can attain, but not uphold. Forcing yourself to be contribution routinely finances that any mental confusion can slickly act as an vindication to get off-track over again.
In a workplace I do beside healers, the prototypal nighttime I double act participants up and then intentionally set the part by deed them enmeshed in a head-based act. This mimics the widespread condition of their functional day. I consequently flip them into an improvisational show business spectator sport that asks they "mirror" all other's behavior, alternately.Post ads:
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At the end of that crippled (without reviewing what righteous happened), I later have them transport a form and I run them finished an effort that prepares them for exploitable near others.
Technically, it's an exertion in moving their cognitive state to their hearts, but utmost important, it is an exert that identifies the jam of occurrence coming up as a partisan instance to slot in beside another human one in employ to therapeutic. It is all active the postulation of conscious rapt to a well-defined interval that has a beginning, interior and end.
Then, I launch the participants into the same strict travail with their very partners.Post ads:
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At its end, we hoop up and discuss the differences scheduling made. They are more often than not huge! The "task" of the exertion involves mirroring observations of conduct. The prototypic smoothed terribly constricted and rapid observations are ordinarily ready-made. The 2nd round, however, participants unconcealed nuances of behaviour (in themselves AND their partners!) that had seemed all unseeable up to that time. Their levels of basic cognitive process in the jiffy built considerably.
Why? The merely article that denatured was they took the case to become conscious. I edward teach one outline of attaining a put across of existence that is positive of connection, and later cheer the participants to will their own. The foundation formation is attractive the event to get immediate near what you are doing previously you set off doing it. This may be simple, but it's something most of us, slightly frankly, don't judge we have the occurrence to do.
But we do, if we clear it. And it doesn't call for that noticeably. A quiet, secluded dump for righteous a few transactions where you can breathe, bob off the contiguous tension, breathe a few more than and consequently in silence say to yourself whatever moves you - an affirmation, a prayer, a poem - to inform you why you are choosing to be within near that character. And then, you walk into the person's presence; waiting to focussing your brimming fame on the moments you are unneurotic.
In this exceptional state of the understanding you are granting your thorough and united limelight to the personage in front of you. But it's freshly as big to found that the time of this aggravated absorption will be limited, both to you and them. Many healers I practise near cause it definite to their charges, "I have (X) magnitude of time to tender you my complete attention, and next I will need to nudge into a contrasting way." This, too, is an inherent subdivision of preparation, for both of you.
We normally unease that by conjuration what amounts to the force of kindness we will not be able to spin it off and dislocate on to the more than head-centered necessities of our activity. It's simply not true.
We were designed to be able to move relating both worlds. This is a contractile organ you can elbow grease and highlight. At original it takes a particular level of action. But as you get used to it, you'll cram how to clap to riddled public eye only by making the choice, and consequently disengaging by fashioning another one.


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Snug fit, excellent look
Reviewed By Daniel C (WESLEY CHAPEL, FL) Reviewed for a 2010 Mazda 3 —6/21/2017 10:04 AM
Perfect fit, high quality, and look great. 2010 Mazdaspeed 3
Reviewed By Jacqueline G (Naperville, IL) Reviewed for a 2017 Mazda 3 —6/15/2017 1:09 PM
I was impressed by how well molded the Husky Liners WeatherBeater Floor Liners fit both the front and rear seating areas of my 2017 Mazda 3 Hatchback. The front liners lock into place, so they do not shift.


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TORONTO – Canada's House of Commons voted Tuesday to not extend provisions in the country's anti-terrorism legislation that allow for preventive arrests and forced testimony.
The ruling conservative government wanted to extend the pieces of legislation that were set to expire on Thursday, but Prime Minister Stephen Harper's motion was defeated 159-124 in the House of Commons.
The two measures are part of the previous Liberal government's response to the Sept. 11, 2001, terrorist attacks on the United States. They empower authorities to arrest and detain suspects for three days without charge and to compel individuals with knowledge of terrorist activity to testify before a judge.
Neither piece of legislation has ever been applied.
It's the second time in a week Canada has struck down portions of its anti-terror legislation. One of Canada's most contentious anti-terrorism measures was struck down Friday by the Supreme Court, which declared it unconstitutional to detain foreign terror suspects indefinitely while the courts review their deportation orders.
Opposition Liberal Leader Stephane Dion said they are an unnecessary infringement on civil liberties. Dion rejected conservative Tory charges that he is soft on terrorism.
"These two provisions especially have done nothing to fight against terrorism, have not been helpful and have continued to create some risk for civil liberties," Dion said.
Maureen Basnicki, whose husband Ken was one of 24 Canadians killed in Manhattan's twin towers, said Members of Parliament would seriously diminish Canada's capacity to fight terrorism if they do not vote to extend the provisions.
"We want to protect other Canadians from the devastation that we experienced," Basnicki said.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
JotForm Support
Hello, qqqqqqqq. You can insert this CSS code (CSS injection) to set your form on a right-to-left display:
direction: rtl;
You can read this user guide to know how to do CSS injection on your forms.
If you need further assistance or if you have other questions or concerns, please do let us know.
JotForm Support
Inside of the form templates gallery I created one template form that you can use.
Inside of that form I used Custom CSS that will enable Arabic language from right to left. Custom CSS used inside of this form can also be applied to Hebrew language.
You can find my form inside of the Form Templates gallery:


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Weights & photos: Anthony Joshua vs. Dominic Breazeale
The weights are in for Saturday's Anthony Joshua vs. Dominic Breazeale headlined card taking place in London. Here in the U.S., Joshua vs. Breazeale will be shown live at 5:15pm eastern on Showtime. Find the weights and photos for the event here. IBF Heavyweight World Championship – Joshua vs. Breazeale Weights Anthony Joshua – 243 ¼ Pounds Dominic Breazeale – 255...
Via Pro Boxing Fans
1 day ago | Discuss
Tony Luis: Derry Mathews did enough to survive, but not win
Canadian lightweight Tony "Lightning" Luis (19-3, 7 KOs) says he's disappointed with the scoring of his unanimous decision loss last weekend to Derry Mathews (38-9-2, 20 KOs) in Matthews' hometown of Liverpool, England, but won't let it deter him from his goals. Despite taking the fight on three days' notice, Luis seemed to outwork Matthews in most rounds and would probably...
Via Pro Boxing Fans
April 21, 2015 | Discuss
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Horse Boarding, indoor, new stalls, new tackrooms, run ins in all 7 pastures, near and with in 30 min of Gloucester County, cumberland county, Delaware…
Standing Dirty Teddy & The Razors Edge Boarding & Breeding & Saddle Shop Horse'N Around Tack Shop, Inc.
Small, semi private farm in Pemberton, NJ. Sales, boarding, & layups offered. Hunters,jumpers, dressage, eventers welcome!
We are a small, family run farm. We breed, board, buy & sell registered mini's.
Eventing and Dressage training. Excellent in starting young horses for any discipline, lessons, full training, coaching, sales. Active show schedule…
Breeders of Welsh Sec. B ponies and crosses
Family Owned and Operated Horsefarm Quarter Horses & Natural Horsemanship Standing at Stud Olenas Blonde Alibi


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Wednesday, February 18, 2015
Portable USB LED Bulb Emergency Lamp
You never know when an emergency situation will come up. If there was a sudden blackout, it always helps to have an Emergency Portable USB LED Lamp! You don't even need batteries, just a power bank! Camping is always fun and sometimes you need to have a light, so this Portable USB LED Light Bulb would come in very handy for camping. You can use it anywhere! It's a warm white light, that's not overpowering. You can plug it into a power bank, computer, laptop or a cellphone charger adapter.
Being Prepared, With A Emergency USB LED Light Bulb!For this review, I received one Onite Portable USB LED Emergency Light Bulb! This is one of the neatest lights. You can use it anywhere, so it comes in very handy! Got a dark closet, shed, or someplace else you could use a light? This is a very practical light that's very convenient. It has a mental hook, so you can hang it up and take down anytime. Plus, this AWESOME light even has an on and off switch! I would highly recommend the Onite Portable USB LED Emergency Light Bulb!
Technical Details:
- Material: PC+Aluminum plate
- Input voltage: 5V
- Bulb size: 60*60*92mm
- Beam Angle: 270°
- Power: 5W LED =50 W halogen Light
- The lamp bead mode: 5730 chips
- Chips Number: 10 chips
- Amount of luminous flux: 5W=400 lm
- Color temperature: 3000K(Warm White)
- Service life: 35000 hours
- Wire length: 1.7 meters
- Individual net weight: 0.078kg
I received one or more of the products mentioned above for free using Tomoson.com. Regardless, I only recommend products or services I use personally and believe will be good for my readers.
Bonny Sallee
Posted by Bonny Sallee at 2/18/2015 09:07:00 PM
Labels: Emergencies, Emergency Gear, Lights, Reviews, USB Lights
1 Awesome Comments! Thanks for visiting!:
- Jerry Marquardt said...
I would like to thank you for the share of this very basic but very needed emergency item.
- 7:19 PM, February 25, 2015
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A 44-year old woman with recurrent solitary fibrous tumor (SFT)/hemangiopericytoma was enrolled in a clinical sequencing program including whole exome and transcriptome sequencing. A gene fusion of the transcriptional repressor NAB2 with the transcriptional activator STAT6 was detected. Transcriptome sequencing of 27 additional SFTs all revealed the presence of a NAB2-STAT6 gene fusion. Using RT-PCR and sequencing, we detected this fusion in 51 of 51 SFTs, indicating high levels of recurrence. Expression of NAB2-STAT6 fusion proteins was confirmed in SFT, and the predicted fusion products harbor the early growth response (EGR)-binding domain of NAB2 fused to the activation domain of STAT6. Overexpression of the NAB2-STAT6 gene fusion induced proliferation in cultured cells and activated EGR-responsive genes. These studies establish NAB2-STAT6 as the defining driver mutation of SFT and provide an example of how neoplasia can be initiated by converting a transcriptional repressor of mitogenic pathways into a transcriptional activator.
Comprehensive clinical sequencing programs for cancer patients have been initiated at several medical centers including our own1–3. In addition to the potential for identifying actionable therapeutic targets in cancer patients, these clinical sequencing efforts may lead to the identification of novel "driver" mutations that might be relatively rare in a common cancer type or be newly found in relatively rare cancer types. In this study, a patient with cellular solitary fibrous tumor/hemangiopericytoma (SFT/HPC) was enrolled in our clinical sequencing project called MI-ONCOSEQ (the Michigan Oncology Sequencing Program). SFT represents a wide spectrum of tumor types of mesenchymal origin that can affect virtually any region of the body4. SFT is composed of CD34-positive fibroblastic-appearing cells, arranged in a distinctive patternless growth of alternating cellularity and collagenous stroma. Whereas most SFTs are benign and can be cured with surgery, 15–20% of patients progress with either local recurrence or distant metastases, which can be difficult to treat4,5. It is unclear whether SFTs originating at diverse sites such as the meninges, lung, and breast share a common pathogenesis.
The index patient was a 44 year-old woman who had surgery and post-operative radiation for an anaplastic meningioma in 2002. Later review reclassified this tumor as a meningeal malignant SFT. In 2009, magnetic resonance imaging (MRI) showed a new brain mass and also a paraspinal mass. Laminectomy was performed, and review of the tissue showed metastatic SFT, which was strongly immunoreactive for CD34. In 2011, the patient was enrolled in the MI-ONCOSEQ integrated cancer sequencing program (Supplementary Fig. 1a) after progression of sarcoma on chemotherapy. Computed tomography (CT)-guided core needle biopsies were obtained from a metastatic site in the liver (Fig. 1a). The specimen showed the typical morphologic features of SFT with HPC-like vessels, collagenous stroma, and patternless architecture of spindled-to-ovoid tumor cells (Figs. 1b,c). Immunostaining for CD34 was positive in the tumor cells and in the endothelial cells, highlighting branching vessels (Fig. 1d). The biopsy cores used for molecular analysis had over 70% tumor cell content based on morphologic analysis.
High quality DNA and RNA was isolated from the core needle biopsies and subjected to next generation sequencing. Whole-exome sequencing of the tumor and matched normal from MO_1005 identified 14 nonsynonymous point mutations (Supplementary Table 1). No significant germline aberrations or somatic point mutations were identified in genes frequently mutated in cancer such as TP53, KRAS, BRAF, or PIK3CA among others. The exome data coupled with single-nucleotide variant (SNV) candidate modeling was used to estimate tumor content of the biopsy specimen at 70% corroborating the histologic assessment (Supplementary Fig. 2). A global landscape of somatic copy number alterations was generated from exome sequencing data (Fig. 1e), and there were only a few regions of substantial copy-number gain or loss (Supplementary Tables 2 and 3). A focal 56-kb one-copy deletion observed in the STAT6 locus (Fig. 1e and Supplementary Fig. 3). Notably, paired-end transcriptome sequencing of RNA identified an intrachromosomal fusion between NAB2 and STAT6 (Fig. 1f). The NAB2-STAT6 fusion was represented by 1,104 paired-end reads either spanning or encompassing the fusion junction of exon 6 of NAB2 to exon 18 of STAT6. In the normal genome, NAB2 and STAT6 are adjacent genes on chromosome 12q13 that are transcribed in opposite directions.
Using primers within exon 6 of NAB2 and exon 19 of STAT6, we confirmed the NAB2-STAT6 fusion in the index case by RT-PCR followed by Sanger sequencing of the amplified product (Fig. 2a). To confirm that the fusion resulted from a DNA-level rearrangement, we carried out long-range PCR of genomic DNA. A 1.3-kb product was obtained specifically in the tumor from the index subject and not in the matched normal tissue (Fig. 2b, left). This allowed us to specifically map the genomic breakpoint of the NAB2-STAT6 fusion (Fig. 2b, right) and confirmed that a genomic inversion occurs at the Chr12q13 locus, fusing NAB2 and STAT6 in a common direction of transcription.
To determine whether the NAB2-STAT6 fusion was recurrent, we interrogated 51 cases of SFT, malignant and benign, from a range of anatomical sites (Table 1 and Supplementary Fig. 1b). We analyzed a total of 27 cases of SFT by transcriptome sequencing. All 27 cases displayed high levels of a NAB2-STAT6 gene fusion, with some variation in the precise exon structure of the fusions (Table 1 and Supplementary Table 4). The number of paired-end reads spanning or encompassing the fusion ranged from 25 to 4,483 per case. In each case, exon 2, 4, 6, or 7 of NAB2 was fused in frame to exon 2, 3, 5, 6,17, or 18 of STAT6. Most of the transcript fusions were at defined exon boundaries, but a small set had fusion junctions within exons (Table 1). Representative fusions are shown in Fig. 2c. SFT-3 is an example of a complex fusion, with a 72 bp fragment derived from the 3′ UTR OF NAB2 and intron 16 of STAT6 at the fusion junction, retaining the reading frame. RT-PCR combined with capillary sequencing was carried out on an additional 24 cases of SFT from the Memorial Sloan-Kettering Cancer Center (MSKCC), and all cases were also positive for a NAB2-STAT6 fusion (Table 1). The presence of the fusion in selected cases was further confirmed by RT-PCR (qRT-PCR) analysis (Supplementary Fig. 4). Thus, regardless of anatomic site of origin or malignant versus benign status, all 51 cases of SFT harbored a NAB2-STAT6 gene fusion. As all of the NAB2-STAT6 gene fusions identified contained 3′ exons of STAT6, we took advantage of an Affymetrix gene expression data set of soft-tissue sarcomas that included a 3′ probe to STAT6 (U133A, probe set 201331_s_at) to assess expression. Importantly, 100% of the SFTs (24 out of 24) expressed the 3′ exons of STAT6 as compared to 28 other sarcomas all with very low STAT6 abundance (Supplementary Fig. 5). RNA sequencing (RNA-seq) and RT-PCR experiments showed the presence of reciprocal STAT6-NAB2 fusion transcripts in only 25 of 51 cases. The absence of a reciprocal transcript in the index case is consistent with the deletion of the 5′ exons of STAT6 (Supplementary Fig. 3). The reciprocal transcripts varied by case, ranging from exact STAT6-NAB2 reciprocals to shorter variants, missing one to several STAT6 exons, both in and out of frame.
NAB2 is comprised of an N-terminal EGR1 binding domain (EBD), a NAB conserved region 2 (NCD2) and a C-terminal transcriptional repressor domain (RD). STAT6 is comprised of a DNA-binding domain (DBD), SH2 domain, and a C-terminal transcriptional activation domain (TAD). The domain structures of the wild-type NAB2 and STAT6 proteins as well as of six representative NAB2-STAT6 fusion proteins identified by transcriptome sequencing in this study are shown (Fig. 3a). A common feature of all of the NAB2-STAT6 fusion proteins was variable truncation in the RD motif of NAB2, which was then minimally fused to the TAD motif of STAT6.
Expression of the predicted NAB2-STAT6 fusion protein products was confirmed by immunoblot analysis of three cases of SFT (Fig. 3b). An antibody to the C-terminus of STAT6, present in all fusions, detected the respective fusion proteins only in the tumor samples, whereas matched normal tissues expressed only wild-type STAT6. SFT-10 and SFT-44 exhibit a common fusion variant, whereas SFT-14 had a larger variant similar to that in SFT-31 (Fig. 3a). Similarly, immunofluorescence using this antibody to the C terminus of STAT6 showed strong nuclear staining (Fig. 3c). Immunofluorescence using an antibody to STAT6 phosphorylated at Tyr641 showed a complete absence of phosphorylation in the eight SFTs tested, whereas nuclear staining was evident in the entrapped endothelial cells or adjacent lung parenchyma (Supplementary Fig. 6). This pattern of C-terminal STAT6 nuclear staining and absence of nuclear phosphorylated STAT6 in SFTs is consistent with the NAB2-STAT6 fusion protein rather than activated wild-type STAT6.
The NAB2-STAT6 fusion allele from the index case (MO_1005) was cloned into a lentiviral vector with a FLAG epitope tag. Benign prostate RWPE-1 cells were infected with control virus (empty vector) or with virus encoding NAB2-STAT6, and pooled stable cell lines were generated. Stable cell lines expressing high and low levels of NAB2-STAT6 were characterized (Fig. 3d). Notably, the cell line with high NAB2-STAT6 expression showed markedly proliferation compared to the cells transduced with control virus, whereas the cell line with low NAB2-STAT6 expression showed an intermediate level of proliferation (Fig. 3e). The proliferation of both NAB2-STAT6-expressing cell lines could be inhibited by small interfering RNA (siRNA) knockdown of EGR1 expression, whereas the cell line transduced with control virus was unaffected (Supplementary Fig. 7). As wild-type NAB2 is a well-characterized repressor of EGR1 transcriptional activity6,7, we measured the expression of established EGR1 target genes in the cell lines stably expressing NAB2-STAT6 (Fig. 3f)8. In contrast to the known activity of NAB2, the NAB2-STAT6 fusion induced expression of EGR1 target genes. This was further verified using an EGR1 response element reporter assay (Fig. 4a). Expression of EGR1 induced expression of the reporter gene by over 200-fold compared to vector control. This induction could be repressed over 90% by coexpression of wild type NAB2 repressor. Expression of the NAB2-STAT6 fusion resulted in the opposite effect. Expression was elevated tenfold over EGR1 alone, confirming that the fusion protein functions as an activator of EGR1 targeted transcription. No significant effect was seen in parallel experiments using a STAT6 response element reporter, showing that the NAB2-STAT6 fusion functions through EGR1 target genes rather than STAT6 target genes. The interaction of the NAB2-STAT6 fusion with EGR1 was further confirmed by chromatin immunoprecipitation (ChIP)-PCR of known EGR1 target gene promoters, in cells transiently expressing of Flag-tagged NAB2-STAT6 (Fig. 4b). Cells receiving vector control showeded no specific enrichment, whereas the NAB2-STAT6 fusion showed specific enrichment for the binding of known EGR1-responsive sites and not for negative control sites. Furthermore, the physical interaction of the NAB2-STAT6 fusion protein with EGR1 could be demonstrated by coimmunoprecipitation (Supplementary Fig. 8).
To investigate the role of the NAB2-STAT6 fusion in determining the transcriptional pattern of SFT, we curated a robust set of EGR1 target genes from an EGR1 ChIP-Seq experiment in K562 cells (GSM803414)9. We extracted the sets of highly enriched peaks from this dataset (at 5%, 10%, and 25% percentiles) and performed initial analysis of these peaks using the GREAT bioinformatics tool10. For example, EGR1 peaks with scores in the top 5% (918 peaks) were mapped to 1,222 genes (some peaks mapped to more than 1 gene) and these 1,222 genes were highly enriched for the known early growth response DNA binding motifs (Supplementary Fig. 9). To assess whether SFT gene expression profiles were enriched for this set of EGR1 target genes, we used Affymetrix U133A microarray data from 23 SFTs and 34 non-SFT soft tissue sarcomas spanning seven subtypes11. We then compared the gene expression profiles of SFT versus non-SFT sarcomas across our EGR1 target gene lists using gene set enrichment analysis (GSEA)12. Indeed, we observed a positive and highly significant enrichment of EGR1 target genes in SFT compared to non-SFT sarcomas (Supplementary Fig. 10). The list of EGR1 target genes in the top 5% was the most highly enriched in SFT tumors (enrichment score (ES) = 0.35, P value < 0.001, false discovery rate (FDR) q value = 0.025, family-wise error rate (FWER) P value = 0.006). Overall, the set of genes differentially expressed between SFT and other sarcomas was significantly enriched for EGR1 target genes. RNA-seq analysis of 7 SFTs compared with 282 other tumor samples also showed high-level expression of EGR1 target genes. We found that EGR1 target genes, including NAB2, NAB1, IGF2, FGF2, PDGFD, and receptor tyrosine kinases like FGFR1 and NTRK1, all had outlier expression levels in SFTs relative to other tumor types (Fig. 4c, Supplementary Tables 5 and 6).
Recurrence analysis on an independent set of tumor samples suggested that nearly all SFTs (100% in this study) harbor a NAB2-STAT6 fusion. This would suggest that the NAB2-STAT6 gene fusion is pathognomonic for SFT and that the spectra of SFT characteristics and morphology have a common genetic origin. Assessment of NAB2-STAT6 fusion status could be used as a genetic marker in sarcoma cases that are not unambiguously classified as SFT (e.g. cases of CD34-negative SFT, and malignant and de-differentiated SFT)13. Although the structure of the fusion proteins varies in individual SFT cases, all fusion proteins have a truncation of the transcriptional repressor domain of NAB2 with an in-frame fusion to the transcriptional activation domain of STAT6 (although additional STAT6 domains may be included). The truncation of the repressor domain attenuates its repressive activity, while addition of a strong, intact activation domain engenders transcriptional activation potential.
How does the NAB2-STAT6 fusion potentially explain neoplastic progression in SFT? NAB2 is a well-known co-regulator of the EGR transcription factors7, and all of the fusion proteins identified in SFT maintain an intact N-terminal EBD. EGR1 is a zinc-finger transcription factor that couples growth factor signaling with the induction of nuclear programs of differentiation and proliferation, which are mediated by EGR1 target genes (Fig. 4d)14. As part of a homeostatic loop, NAB2 is induced by EGR family members and functions in a negative feedback manner to repress their activity15,16. In the context of SFT, the NAB2 fusion inherits an activation domain from the signaling molecule STAT6, which converts a transcriptional repressor (NAB2) into a potent transcriptional activator (i.e., NAB2-STAT6) of EGR1. This leads to constitutive activation of EGR mediated transcription, culminating in a feedforward loop that drives neoplastic progression. We found that EGR target genes including NAB2, NAB1, IGF2, FGF2, PDGFD, and receptor tyrosine kinases like FGFR1 and NTRK1, all exhibited outlier levels in SFTs relative to other tumor types (Fig. 4c). Furthermore, a number of kinases including FGFR1 are targets of EGR1 and are also overexpressed in SFT and may be explained by the feedforward loop potentiated by the NAB2-STAT6 fusion. Although it will be a challenge to target the NAB2-STAT6 fusion protein therapeutically, some of the downstream kinases it induces may serve as attractive drug targets that should be evaluated in clinical trials for SFT. A clinical study that tested the kinase inhibitor sunitinib and the figitumimab antibody to IGF1R in SFT showed some positive responses17.
Taken together, this study implicates aberrations in NAB2 and STAT6 in neoplastic pathways of virtually all SFTs, both benign and malignant, and may predict a role for these genes in other more common tumor types, as cancer genome sequencing efforts continue. Integrative sequencing provides a molecular definition of cancers to supplement and clarify histopathological characterizations18. In addition to suggesting actionable mutations, unbiased clinical sequencing efforts may shed light into the biology of rare cancers or individual cases of more common cancer types. To our knowledge, this is one of the first examples of how a gene fusion can convert a transcriptional repressor (NAB2) into a transcriptional activator (NAB2-STAT6) of mitogenic pathways that can be subverted during neoplastic progression.
Research was performed under institutional review board (IRB)–approved studies. Patients are enrolled and consented through a University of Michigan IRB-approved protocol for integrative tumor sequencing, MI-ONCOSEQ (Michigan Oncology Sequencing Protocol, IRB# HUM00046018)1. Medically fit patients 18 years or older with advanced or refractory cancer were eligible for the study. Informed consent detailed the risks of integrative sequencing and included up-front genetic counseling. Biopsies are arranged for safely accessible tumor sites. Needle biopsies were snap frozen in OCT and a longitudinal section was cut. Hematoxylin and eosin (H&E) stained frozen sections were reviewed by study pathologist (L.P.K.) to identify cores with highest tumor content. Remaining portions of each needle biopsy core were retained for nucleic acid extraction.
Thirty SFTs with available frozen tissue material from MSKCC files were included for analysis. Seventeen of the SFT were previously analyzed as part of a prior gene expression profiling study and CEL files have been made publicly available11. There were 16 females and 14 males with a wide age range at diagnosis (12–79 years; mean 52 years). The retrospective validation study was approved by IRB (IRB# 02-060).
Genomic DNA from frozen needle biopsies and blood was isolated using the Qiagen DNeasy Blood & Tissue Kit, according to the manufacturer's instructions. Total RNA was extracted from frozen needle biopsies (for RNA-Seq libraries, gene expression analysis and RT-PCR) using the Qiazol reagent with disruption using a 5mm bead on a Tissuelyser II (Qiagen). RNA was purified using a miRNeasy kit (Qiagen) according to the manufacturer's instructions. cDNA was synthesized from total RNA using Superscript III (Invitrogen) and random primers (Invitrogen). For the MSKCC samples, total RNA was extracted from frozen tumor tissue using the Trizol reagent according to the manufacturer's instructions (Invitrogen).
Exome libraries of matched pairs of tumor/normal genomic DNAs were generated using the Illumina TruSeq DNA Sample Prep Kit, following the manufacturer's instructions. RNA-Seq transcriptome libraries were prepared following Illumina's TruSeq RNA protocol, using 2μg of total RNA. PolyA+ RNA was isolated using Sera-Mag oligo(dT) beads (Thermo Scientific) and fragmented with the Ambion Fragmentation Reagents kit (Ambion, Austin, TX). cDNA synthesis, end-repair, A-base addition, and ligation of the Illumina indexed adapters were performed according to Illumina's protocol. Libraries were then size-selected for 250–300 bp cDNA fragments on a 3% Nusieve 3:1 (Lonza) agarose gel, recovered using QIAEX II gel extraction reagents (Qiagen), and PCR-amplified using Phusion DNA polymerase (New England Biolabs) for 14 PCR cycles. Paired-end libraries were sequenced with the Illumina HiSeq 2000, (2 X100 nucleotide read length). Reads that passed the chastity filter of Illumina BaseCall software were used for subsequent analysis. Summary sequencing statistics are presented in Supplementary Table 7.
We used the publicly available software FastQC to assess sequence quality. For each lane, we examine per-base quality scores across the length of the reads. Lanes were deemed passing if the per-base quality score boxplot indicated that >75% of the reads had >Q20 for bases 1–80. All lanes passed this threshold. In addition to the raw sequence quality, we also assess alignment quality using the Picard package.
We annotated the resulting somatic mutations using RefSeq transcripts. HUGO gene names were used. For NAB2 mRNA and protein, positions and annotations are derived from RefSeq accessions NM_005967 and NP_005958 respectively. For STAT6 mRNA and protein, positions and annotations are derived from RefSeq accessions NM_001178078 and NP_001171549 respectively. The impact of coding non-synonymous amino acid substitutions on the structure and function of a protein was assessed using PolyPhen-267. We also assessed whether the somatic variant was previously reported in dbSNP135 or COSMIC v5668.
Copy number aberrations were quantified and reported for each gene as the segmented normalized log2-transformed exon coverage ratios between each tumor sample and matched normal sample19. To account for observed associations between coverage ratios and variation in GC content across the genome, lowess normalization was used to correct per-exon coverage ratios prior to segmentation analysis. Specifically, mean GC percentage was computed for each targeted region, and a lowess curve was fit to the scatterplot of log2-coverage ratios vs. mean GC content across the targeted exome using the lowess function in R (version 2.13.1) with smoothing parameter f=0.05.
Somatic point mutations were identified in the tumor exome sequence data using the matched normal exome data to eliminate germline polymorphisms. Parameters and computational methods were as previously described20.
To identify gene fusions, paired-end transcriptome reads passing filter were mapped to the human reference genome (hg19) and UCSC genes, allowing up to two mismatches, with Illumina ELAND software and Bowtie21. Sequence alignments were subsequently processed to nominate gene fusions using the methods described earlier22,23. In brief, paired end reads were processed to identify any that either contained or spanned a fusion junction. Encompassing paired reads refer to those in which each read aligns to an independent transcript, thereby encompassing the fusion junction. Spanning mate pairs refer to those in which one sequence read aligns to a gene and its paired-end spans the fusion junction. Both categories undergo a series of filtering steps to remove false positives before being merged together to generate the final chimera nominations. Reads supporting each fusion were realigned using BLAT (UCSC Genome Browser) to reconfirm the fusion breakpoint.
For RNA-Seq gene expression analysis, transcriptome data was processed as previously described1.
For validation of fusion transcripts, RT-PCR and quantitative RT-PCR assays were performed. One microgram of total RNA from 30 SFT was used for RT-PCR using SuperScript III First-Strand System (Invitrogen), according to the manufacturer's instructions. The primers used were: NAB2ex5 Forward and STAT6ex20 Reverse (Supplementary Table 8). The PCR products were analyzed by agarose gel electrophoresis. The amplified PCR products were purified then sequenced using the Sanger method. Quantitative RT-PCR assay was performed using SYBR Green Master Mix (Applied Biosystems) and was carried out with the StepOne Real-Time PCR System (Applied Biosystems). Relative mRNA levels of the fusion transcripts were normalized to the expression of the housekeeping gene GAPDH. Oligonucleotide primers were obtained from Integrated DNA Technologies (IDT) and the sequences given in Supplementary Table 8. To detect the genomic fusion junction between the NAB2 and STAT6 genes in the MO_1005 tumor DNA, primers were designed flanking the predicted genomic junction and PCR reactions were carried out to amplify the fusion fragments. PCR products were purified from agarose gels using the QIAEX II system (QIAGEN) and sequenced by Sanger sequencing.
Using similar conditions as above, RT-PCR for detection of STAT6-NAB2 reciprocal transcripts was performed on all 30 SFT cases and depending on the expected fusion transcript two primer pairs were used: STAT6 Ex13F and NAB2 Ex7R or STAT6 Ex1F and NAB2 Ex7R (Supplementary Table 8).
Total protein lysates were extracted from frozen tissue from 8 SFT tumors. In three of the cases, adequate quality frozen normal tissues were available for protein extraction for comparison. Electrophoresis and immunoblotting were performed using 30 μg of total protein extract, following the standard protocol. Total STAT6 and β-actin were detected by rabbit polyclonal anti-STAT6 (Cell Signaling Technology, Cat #9362S; 1:1500 dilution) and rabbit monoclonal anti-β-actin (Cell Signaling Technology, Cat #4970; 1:1500 dilution). The secondary antibodies used were goat anti-rabbit (Santa Cruz Biotechnology, Cat #SC-2034) with 1:20000 dilution. The same total STAT6 antibody was used for immunofluorescence (IF) for detecting the cellular localization of the protein. IF for detecting Phospho-STAT6 was performed using the P-STAT6 Y641 primary antibody from Cell Signaling (#9361; 1:100 dilution) and a secondary antibody Alexa Fluor 594 goat anti-rabbit IgG from Invitrogen, (#A11037; 4ug/ml).
The NAB2-STAT6 fusion allele was PCR amplified from cDNA of the index case (MO_1005) using the primers listed in Supplementary Table 8 and the Expand High Fidelity protocol (Roche). The PCR product was digested with restriction endonuclease Cpo I (Fermentas) and ligated into the pCDH510B lentiviral vector (System Biosciences), which had been modified to contain an N-terminal FLAG epitope tag. Lentiviruses were produced by cotransfecting the NAB2-STAT6 construct or vector with the ViraPower packaging mix (Invitrogen) into 293T cells using FuGene HD transfection reagent (Roche). Thirty-six hours post-transfection the viral supernatants were harvested, centrifuged at 5,000xg for 30 minutes and then filtered through a 0.45 micron Steriflip filter unit (Millipore). Benign RWPE-1 cells at 30% confluence were infected at an MOI of 20 with the addition of polybrene at 8 μg/ml. Forty-eight hours post-infection, the cells were split and placed into selective media containing 10 μg/ml puromycin. Two stable pools of resistant cells were obtained and analyzed for expression of the FLAG-NAB2-STAT6 fusion allele by western blot analysis with monoclonal anti-FLAG M2 antibody (Sigma-Aldrich). Expression was confirmed by qPCR for the NAB2-STAT6 fusion allele.
For the cell proliferation assay, vector control, NAB2-STAT6 high, and NAB2-STAT6 low level over-expressing cells were plated in quadruplicate at 8,000 cells per well in 24 well plates. The plates were incubated at 37°C and 5% CO2 atmosphere using the IncuCyte live-cell imaging system (Essen Biosciences). Cell proliferation was assessed by kinetic imaging confluence measurements at 3-hour time intervals.
We grew 293T cells to 70–80% confluence in DMEM supplemented with 10% fetal bovine serum, followed by transfection with EGR1-myc alone, or co-transfection with EGR1-myc and Flag-NAB2-STAT6 using FuGene6 reagent (Roche). Twenty-four hours after transfection, cells were cross-linked with 1% formaldehyde, neutralized with 0.125M glycine, rinsed twice with ice-cold PBS buffer, and cell pellets were collected. ChIP-seq was performed as previously described 24.
293T cells were grown to ~70% confluence in DMEM supplemented with 10% fetal bovine serum, followed by transfection with myc-tagged EGR1 alone, or co-transfection with EGR1-myc and Flag-tagged NAB2-STAT6 using FuGene6 reagent (Roche). Twenty-four hours after transfection, cells were cross-linked with 1% formaldehyde, neutralized with 0.125M glycine, rinsed twice with ice-cold PBS buffer, and pelleted by centrifugation. Cell pellets were lysed in lysis buffer followed by immunoprecipitation with anti-MYC tag antibody (Sigma) and protein-G Dynabeads (Invitrogen). Precipitates were washed three times with IP Wash buffer resuspended in 3X SDS-PAGE loading buffer, and cooked at 95°C for 20 min to reverse cross-linking by formaldehyde. Flag-NAB2-STAT6 fusion protein was detected by Western blotting with anti-Flag antibody (Sigma).
RWPE-1 stable lines (vector control, NAB2-STAT6 high, and NAB2-STAT6 low) were transfected twice with EGR1-targeting siRNA or non-targeting siRNA (Thermo Scintific Dharmacon) using Oligofectamine (Invitrogen). The siRNAs used were as follows: ON-TARGETplus EGR1 LQ-006526-00-0002 and ON-TARGETplus Non-targeting pool. Twenty-four hours after transfection, cells were trypsinized and plated in quadruplicate at 4,000 cells per well in 24 well plates. The plates were incubated at 37°C with 5% CO2 atmosphere in the IncuCyte live-cell imaging system (Essen Biosciences). Cell proliferation rate was assessed by kinetic imaging confluence measurements at 3-hour time intervals.
293T cells were seeded into 12-well dishes in triplicate and allowed to attach overnight. Cells were serum-starved for 12 hours and transfected with different combinations of EGR1 (Origene), wild-type NAB2 (Origene), and NAB2-STAT6 fusion constructs along with a mixture of EGR1-responsive firefly luciferase construct and constitutively expressing Renilla luciferase construct (SABiosciences). Following incubation for 24 hours, cell lysates were prepared and measured for EGR1 activity using Promega Dual Luciferase reagents and Passive Lysis Buffer. Firefly luciferase levels were normalized using corresponding Renilla luciferase levels for each condition. To test if the NAB2-STAT6 fusion protein functions through the STAT6 signaling pathway, a STAT6-responsive firefly luciferase reporter was constructed in pGL4.10 (Promega) and luciferase activity assays were performed as described above. The sequences of oligos used for the preparation of the STAT6-responsive firefly luciferase construct are listed in Supplementary Table 8.
The authors thank T. Barrette for hardware and database management, S. Birkeland, M. Pierce-Burlingame and K. Giles for assistance with sample and manuscript preparation, and X. Jing for carrying out microarray experiments. We also thank the larger MI-ONCOSEQ team including cancer geneticists S. Gruber, J. Innis, bioethicists J. Scott Roberts and Scott Y. Kim; genetic counselors, J. Everett, J. Long and V. Raymond and radiologists, E. Higgins, E. Caoili, and R. Dunnick. M. Quist performed initial SNV analysis. L. Sam, A. Balbin, and P. Vats assisted with bioinformatics analysis. This project was supported in part by the NCI Early Detection Research Network (U01 CA111275), the National Functional Genomics Center (W81XWH-11-1-0520) supported by the Department of Defense (A.M.C.), and in part by the National Institutes of Health through the University of Michigan's Cancer Center Support Grant (5 P30 CA46592), PO1 CA047179-15A2 (C.R.A., S.S.), P50 CA 140146-01 (C.R.A., S.S.), Linn Fund and Cycle for Survival (C.R.A.), the Alan Rosenthal Fund for research in sarcoma (C.R.A.), and the Weinstein Solitary Fibrous Tumor Research Fund (C.R.A.). A.M.C. is supported by the Doris Duke Charitable Foundation Clinical Scientist Award, and a Burroughs Welcome Foundation Award in Clinical Translational Research. A.M.C. is an American Cancer Society Research Professor and A. Alfred Taubman Scholar.
Conflict of interest: none
Accession codes: The exome and transcriptome sequencing data have been deposited in the databased of Genotype and Phenotypes (dbGAP) under accession phs000567.v1.p1.
AUTHOR CONTRIBUTIONSD.R.R., C.R.A., and A.M.C. conceived the experiments. D.R.R., Y.M.W., and X.C. performed exome and transcriptome sequencing. S.K.S. and M.K.I. carried out bioinformatics analysis of high throughput sequencing data and nomination of gene fusions. R.J.L. carried out bioinformatic analysis of high throughput sequencing data for gene expression, copy number and tumor content determination. Y.S.S., C.L.C., D.R.R., Y.M.W., and F.S. isolated nucleic acids and performed PCR and Sanger sequencing experiments. Y.M.W. and F.S. carried out gene fusion validations and gene fusion cloning. Y.M.W., R.W., F.S., and D.R.R. carried out cell-based in vitro experiments and QPCR assays. L.Z. and C.L.C. performed immunoblot and immunofluorescence experiments on tissue samples. J.S. collected and prepared tissue samples for next generation sequencing. L.P.K., J.M.M., and C.R.A. provided pathology review. S.M.S. and S.S. provided the patient samples and clinical data. S.R., K.J.P., M.T., S.K.S., R.J.L., J.S., D.R.R. Y.M.W., X.C., and A.M.C. developed the integrated clinical sequencing protocol. D.R.R., Y.M.W., C.R.A. and A.M.C. prepared the manuscript, which was reviewed by all authors.
This manuscript was disclosed to the University of Michigan Office of Technology Transfer, which has filed a patent on the findings.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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ncl gem horrible holiday cruise: Norwegian Gem Cruise Review by lamfam126
Overall Member Rating
ncl gem horrible holiday cruise
Destination: Eastern Caribbean
Embarkation: New York (Manhattan)
day 2 day at sea the wether was horrible and the activities were for a much older crowd im not interested in lectures about global warming or jewelry repair eating right gimmie a break im on vacation not in school.We enrolled our 14 yr old in the teen club he was bored out of his mind!!!!!!!!!! he spent most of his time in the arcade at 1 dollar to 3 dollars a game or the bowling alley that we did not know it was 10 bucks per person per game total ripoff, again dinner and then a show , the shows were good thank god ..
day 3 florida ncl did not keep the timetable and we got to fl 3 hrs late which totally screwed the day up .we met up family and had christmas but had to return early so we would not be forced to rent the car for an extra day , which we did because of the 3 hr delay ..but it was nice to see family ..
day 4 was supposed to be great stirrup cay and don't you know we could not tender because the water was too rough (total disappointment and don't you know the nicest weather on the entire cruise) we went right to reception to ask for a revised daily itinerary , thats when i lost it ... no kids activities , no adult activities they decided to paint and the fumes were horrible by day 4 you have seen and done all thats included without paying extra for bowling , casino, shopping the rock wall was never open... we went to dinner to discover it was the same menu as the first night , we were starved and opted for the steakhouse @ 20 bucks per person sat down opened the menu to find another 10 dollar charge for anything on the specials menu!!!!!!!!!!!!!! of course anything good was on the special menu it was good not great just good . we went to a show and sat in the casino we were bored out of our minds this was christmas eve and now i want my suitcase these people are programmed to smile and say sorry not found yet , i had to break down in tears to get something done .. fill out forms and wait till i get home so christmas was ruined, so sympathy and the language barrier was frustrating ..
day 6 christmas morning freeport bahamas i have to say waking up to most of your gifts being misplaced we went to the java cafe and santa was there thats it no music no christmas spirit the dinner menu was horrible and i was not spending one red cent on this ship . we have been to the bahamas before we knew what to expect but did not realize everything would be closed no shops ,restaurants, nada .we went back to the nightmare ship and could not wait to get home ...
day 7 debarkation we opted for the express , carry out your own luggage and we did we ran off that ship ncl does not take care of their guest like carnival does it was the worst cruise ever we could have saved 4 grand and stayed home , i a currently working with the insurance company to out in a claim and wrote to ncl seeking compensation for everything else ....stay with what cruiseline you know we will never ever sail with ncl again!!!!!!!!!!!!!!!!!!!ps the room stewards were horrible had to chase them around to maintain our cabin Less
Published 01/20/09
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text: We'll be in DC over the weekend. Need to scope out places for dinner on Friday and Saturday and lunch on Saturday and Sunday. We will be located near the hotel & the Smithsonian. My children are 3 and 6 and not good eaters. Mostly bread basket scarfers. Are there any places that have both good food for adults and a childrens' menu of boring standards (chicken nuggets - cringe)? thanks


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painted on his house in LA, what did
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painted on his house in LA, what did
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