text:
Dream. Dare. Do – that is Suyati's work principle in a nutshell.
Customer engagement plays a vital role in any business. With the evolution of smartphones and internet playing a major role in our lives today, customer behavior has witnessed lot of changes. Organizations need to respond to these changes by implementing online customer engagement facilities.
Customers are able to access the information, track or share them wherever they are and whenever they want. Hence, it is vital that business firms connect with their customers through their mobile devices to build their relationship and retain them.
To engage effectively with the customers through mobile apps, care should be taken to choose the right app. The following three steps will give a basic idea:
Step 1: Research to know the preferences and likes of your target customers.
Step 2: Choose the right mobile technology and design to attract customers.
Step 3: Analyze if the business firm can re-use an existing mobile technology or develop one of its own.
Step 4: Develop a solution and advertise in the right media to get maximum attention from customers.
There are many advantages business firms see in using mobile for customer engagement process:
Mobile solutions can transform Customer Engagement Process
Mobile solutions are winning over the hearts of customers and enhancing the customer engagement process in many ways. These solutions are basically build over six main strategies:
Following such strategies, customers can benefit from mobile apps and solutions. This is how mobility impacts customer engagement process.


text:
Recently stayed in the Zinfandel Suite for 2 nights and all I can say is wow!!! Beautiful accommodation, large comfy bed, huge bathroom and in the grounds of the owners winery...what more could you want. Originally we had only booked in for 1 night but stayed for 2. Debbie and Jim, the owners treat you so well and you feel very welcome. Breakfast is served in their house next door and is delicious. Everything home made and freshly squeezed orange juice. Was worried about noise given the close proximity to the road but it was not a problem at all. Very handy location for visiting Kings Canyon and Sequoia National Parks. Great restaurant just minute along the road. All in all only one word needed...brilliant!
- Official Description (provided by the hotel):
- Sequoia View B&B, located on 20 acres with an estate vineyard, is named for its panoramic views of nearby Sequoia & Kings Canyon National Parks. Each of the 3 suites has private entrances and baths. Guests enjoy a wine & cheese reception at check-in and a full homemade breakfast in the morning.Visitors have an abundant choice of activities to enjoy due to our location on the Blossom Trail and proximity to the Kings River and national parks. Tour the winery and tasting room and enjoy handcrafted wines made on site by the innkeepers, Jim & Debbie Van Haun. Our Cedar View Winery established in 2001 produces award winning wines, earning medals at local, regional and international competitions. ... more less

subset: commoncrawl


text:
The Polar Network network has installed charging stations in 23 charging area so that you can recharge your electric car.
Required passes :
If you are working for Polar Network, do not hesitate to contact us to automate your changing areas search engine optimization.
ChargeMap and its wonderful community saved more than once from running out of fuel! - Sébastien


text:
CinerGold Toned and Black Metal and Enamel Tiger Pin Brooch
Vintage brooch has pin with c-clasp lock.
Made in: Unknown Color: Gold and Black Fabric Content: Unknown Metal and Enamel Condition: Shows signs of wear. Scratches with some heavy dents throughout metal. Slight tarnishing.
Measurements & Sizing
- Length: 2.5"
- Width: 2.375"
Returns & Shipping
Luxury Garage Sale guarantees that all items sold on our site are 100% authentic. All products we accept are inspected for brand-specific guidelines and are put through a rigorous authenticity test process.
Item #: 88880


text:
services not reconized
I'm using a cisco 3544 chasis with a MC03A Board installed on the primary slot. The MC03A board has successfully registered to call manager(4.2).
I'm using SCCP for video conferencing, I've activated 15 sccp ports, activated sccp services. I've tried using default and creating my own sccp services for testing.
When I try to create a conference I get a message in the prefix box saying: "No services are configured in the mcu."
I have the default prefix's as well as my own self defined prefix's. I need the services(prefix's) to appear in order to initiate a conference.
I'm unsure on how to go about troubleshooting this, any suggestions or advice are appreciated.
If the unit was running 3.x and was upgraded directly to 4.2.10, this may be where the problem lies. You can start by downgrading to 3.5.24 and restoring the unit to factory defaults. Then upgrade to 4.0 then 4.1.9 and finally 4.2.10. Please try to downgrade and upgrade the MCU again. You may have to manually recreate the MCU services since the configuration may
be corrupt


text:
Hey y'all, today I'm showing you how to make knit Christmas pajamas as part of the Riley Blake Stitch'n Kitchen tour, and I've got a FREE pattern, and even a dessert recipe to share too! I know, a lot of stuff, right?
So this post is going to be about the pajamas, and it got really long, so if you want the Candied Pecan Turtles recipe (and these are yuuuuuuummmy) you can find it here.
As soon as I got the invite to do a Christmas tour, I knew what I wanted to do – cousin Christmas pajamas. The cousins loved the Good Natured pajamas I made them last year, and I love the photos, so it seemed natural to do a reprise. A quick look through the Riley Blake catalog and the perfect fabrics presented themselves – the Christmas knit basics. I used Holidays in all three colorways and Christmas Main in pink. Because the lone girl's gotta have pink.
I've been meaning to do a raglan type fitted shirt, so even though it's a little hard to see, that's the typ of sleeve on these. Which means you could totally do a contrast sleeve for fun. But with a short time to make 4 sets, I stuck to my assembly line sewing style and as few fabrics as possible.
Baby girl had fallen flat on her face the day we took these photos, but she was a good sport, even with her wild boy cousins.
Want to make your own? I've got a free pattern for the size 4T pajamas (the ones Baby Girl is wearing). To make your own, you'll need
- 1 1/2 yards of knit fabric. Because this is so fitted, you want to make sure that the fabric has 4 way stretch; this is part of why the Riley Blake knits are perfect since they have 4 way stretch and 5% spandex.
- 1/4 yard of rib knit fabric for the cuffs and neckband – fabric should have at least 80% stretch for the neckline (that means you can stretch a 10 inch piece to 18 inches). You could use the same fabric as the body IF it has good stretch and recovery.
- 19 inches of 1 inch wide elastic
- The pattern. To download the pattern, subscribe to the Melly Sews Weekly Newsletter. AFTER you sign up, you'll get a confirmation email – and you have to confirm or my email service can't send you the free pattern. But once you do confirm, you'll get access to not just this but many more free patterns. Woot! (PS – if you have trouble finding either your confirmation email or the email with the pattern link, try checking your spam, trash, junk, bulk, social and/or promotional email tabs or folders. Free pattern links are also included in every weekly newsletter; these go out on Friday afternoons. Due to the number of subscribers I can't email pattern links to individuals.)
Please note that all my patterns are licensed for personal use only (no selling items made from this) and by downloading you are agreeing to this license.
This is for a kids size 4T. If you need a different size, you're in luck! I've got sizes 18m-8 available for sale here or by using the button below.
Cut out two sleeves, a front, a back, two legs, a neckband, two sleeve cuffs and two leg cuffs.
Use 1/2″ seams and a stretch stitch unless otherwise indicated. See this post for tips on sewing knits.
Place a sleeve right sides together with the shirt front. The front edge of the sleeve is shorter than the back edge. Match raw edges and stitch seams. Repeat with other sleeve.
Stitch the sleeves right sides together with the shirt back, so that you have a piece that looks like the picture above.
Fold the shirt right sides together, matching sleeve seams. Stitch the underarm and side seams in one seam on each side as shown below.
Fold the cuffs right sides together, matching side edges (side edges are those at the sides when the text is right side up). Stitch. Roll up one side of the cuff over the other, wrong sides together, so that you have a loop with right sides out on both the inside and outside. Repeat this process with the neckband.
Pin the neckband around the neckline, matching the center front and center back, stretching the neckband to meet the neckline but no more. You'll be pinning through 3 layers of fabric – two of the neckband, one of the neckline. Stitch, using a stretch stitch.
Repeat this process with the sleeve cuffs, matching the seam to the sleeve seam. It can be easier to stitch this small opening by putting the machine needle inside the cuff circle.
Place the two pants pieces right sides together and stitch the curved crotch seams with a stretch stitch as shown above.
Fold the pants to match the crotch seams. Sew along the inseam in one long seam, using a stretch stitch.
Fold the waistband down 1 1/4 inches, then stitch a casing for the elastic, leaving an opening to insert the elastic. Using a safety pin or bodkin, insert elastic and sew into a loop, then sew the opening in the casing closed.
Sew the pant cuffs as you did for the sleeve cuffs, then sew them to the pant legs as you did with the sleeve cuffs.
And then see if you can wrangle a little kid photo shoot!
Happy sewing,


text:
Join Date: Dec 2009
Posts: 87
Mentioned: 0 Post(s)
Tagged: 0 Thread(s)
Quoted: 0 Post(s)
|Thread Tools
I do use snapfish and create photobooks online.
I make sure to print off pictures every month and at least stick them in a photo album. I take maybe an hour over the course of a few days and jot notes next to each picture, so I don't forget those moments. Then I set up project times (like next month when I have a 3 week break from school) that's when I make online photobooks and edit to make them really special.
So I'm hearing that you not only print out hard copes of each photos, but you also create a photobook print copy too? Is that right that you do both? So you would have 2 hard copies of every photo?
I didn't want to print out all the photos just because they are too much (and it'll be taking up a lot of space as they add up), but I don't really have a way of jotting down notes next to the digital photos so I can wait until I can sit down and compile the photobook with those note/journals later. Any suggestions on being able to easily jot down some quite notes about the moments associated to specific photos without printing out the photos? (I want to use the notes when I actually compile the whole photobook)
Another question, how often do you create a photobook? My first photobook consisted of my child's 2-4 month old photo collection (doesn't even have any notes/journals) (I skipped making anything for birth and first month), and then I never did another book again and he is almost 9 months now. Do you have one photobook per, say, 6 months, a year, or what? and how many pages do each of these photobooks have? Just wanted to get an idea of how much photographing you do and how much you make them into products like photobooks.... Because to me all the photos are overwhelming, I don't take photos all the time, but when I do, I take a lot of the same shot to make sure I get a good shot (because the baby moves so much and you can never get a clear shot), and I end up having to screen through possibly 20-30 shots of the same thing trying to pick out a good one).
Mama to (DS 7) and (DD 5), wife to DH
|Thread Tools
Show Printable Version Show Printable Version
Email this Page Email this Page
|Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts
BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are Off
Online Users: 16,662
|25 members and 16,637 guests
|bananabee , BirthFree , blessedwithboys , Deborah , girlspn , greenemami , happy-mama , hillymum , Iron Princess , JElaineB , LadyAnnibal , lhargrave89 , Lydia08 , Michele123 , Mirzam , MountainMamaGC , NaturallyKait , nic1231 , racarmich , rightkindofme , Shmootzi , Skippy918 , Springshowers , sren
|Most users ever online was 449,755, 06-25-2014 at 12:21 PM.


text:
Welcome to MIT Men's Ultimate, a fun and competitive way to get some exercise and have a great time. If you are interested in signing up for the team, please email the officer's list at mens-ult-officers [at] mit.edu. We have provided a brief FAQ to give you a better idea of what's in store, but feel free to shoot us an email if you are thinking about playing.
Q: How much experience is needed to play on the team?
A: We welcome anyone, regardless of their previous experience. Over the course of the year, we will teach you everything you need to know. However, we are a competitive team in the region and we hope you are willing to come to practices and tournaments to improve your own playing as well as the team's.
Q: Do you play other schools? How does being a club sport work?
A: Yes, most major universities have college frisbee teams. In the past, we have played Harvard, Tufts, Boston College, and even schools as far as Ohio State and Georgia State. Being on a club sport is in many ways similar to a varsity sport, though we will welcome anyone regardless of experience.
Q: How much of a time commitment is Ultimate?
A: Typically the team will hold 2-3 practices a week, each 2 hours in length. The amount of practicing generally ramps up towards the beginning of the college season. We practice year round, check the calendar for practice times and dates. At least one weekend per month, we will head to a frisbee tournament to play other colleges – it will be expected that you can make the majority of these as they are an excellent learning experience. We are aware that MIT can be a difficult place so if you have any timing concerns, just send us an email and we will try to work something out.


text:
|Home | About | Journals | Submit | Contact Us | Français
This study was designed to test whether metabolic characterization of intact, unaltered human rectal adenocarcinoma specimens is possible using the high-resolution magic angle spinning proton (1H) magnetic resonance spectroscopy technique.
The study included 23 specimens from five patients referred for ultrasonographic staging of suspected rectal cancer. Multiple biopsies of macroscopically malignant rectal tumors and benign rectal mucosa were obtained from each patient for a total of 14 malignant and 9 benign samples. Unaltered tissue samples were spectroscopically analyzed. Metabolic profiles were established from the spectroscopy data and correlated with histopathologic findings.
Metabolomic profiles represented by principle components of metabolites measured from spectra differentiated between malignant and benign samples and correlated with the volume percent of cancer (P=0.0065 and P=0.02, respectively) and benign epithelium (P=0.0051 and P=0.0255, respectively), and with volume percent of stroma, and inflammation.
Magnetic resonance spectroscopy of rectal biopsies has the ability to metabolically characterize samples and differentiate between pathological features of interest. Future studies should determine its utility in in vivo applications for non-invasive pathologic evaluations of suspicious rectal lesions.
Rectal cancers account for approximately one-third of colorectal malignancies, and the estimated number of cases for 2008 is 40,740.1 The expected overall five-year survival rate for patients with a diagnosis of rectal cancer is 65 percent in the United States, according to the National Cancer Institute.2 Despite known prognostic factors and new and improved diagnostic methods, the severity of cancer stage is frequently underestimated.3 Accurate diagnosis, staging, and management of the disease are crucial for achieving optimal outcomes. Currently, although histopathologic evaluation of biopsy samples can achieve most of these goals, radiologic tests such as computed tomography (CT), ultrasonography (US), positron emission tomography (PET), and magnetic resonance imaging (MRI) are unable to differentiate malignant from benign tissue with sufficient accuracy to be clinically useful for noninvasive diagnosis. The preoperative staging of the tumor is usually done according to the TNM system by using MRI, endorectal US, CT, or a combination of these tests to assess the tumor itself, whether it has spread to locoregional lymph nodes, and whether distant metastases are present.4, 5
The diagnosis of rectal cancer is currently dependent on biopsies taken from the suspicious lesion during colonoscopic or sigmoidoscopic examinations. Although histopathology will remain the standard for the diagnosis of rectal cancer for years to come, it is desirable to develop new technologies that not only can detect suspicious sites on direct biopsy, but may even replace histopathology to achieve diagnosis and grading of rectal cancer tumors noninvasively in vivo. Feasible techniques currently being examined for their potential roles in cancer management are those that probe the underlying biochemistry accompanying malignant changes, such as magnetic resonance spectroscopy (MRS). High-resolution magic angle spinning (HRMAS) proton (1H) MRS is one such technique developed for the metabolomic analysis of intact tissues.6, 7 The HRMAS 1HMRS technique allows acquisition of highly resolved spectra from unaltered tissue samples, while preserving tissue histopathologic architecture for histopathologic analysis. Since its development, several groups, including our own, have shown the ability of this technique to differentiate malignant from benign samples of various tissues and to accurately characterize the bioactivity and, therefore aggressiveness, of tumors.8, 9 The aim of the present study was to test the efficacy of the HRMAS 1HMRS technique in differentiating malignant rectal cancer from benign cells by measuring the metabolic profile of rectal tumor biopsy samples and comparing them with benign rectal mucosa samples from the same patients.
Patients referred for ultrasonographic staging of suspected rectal cancer at the University of Minnesota were asked to participate in the study. The first five patients who agreed to participate were included in the pilot cohort reported here. Written informed consent to obtain ultrasonography-guided multiple biopsies from the tumor and benign rectal mucosa was obtained. Approval from the Institutional Review Board of the University of Minnesota was obtained before study initiation.
Biopsies of untreated rectal lesions that appeared malignant on US examination and on macroscopic examination with rigid sigmoidoscopy were obtained from each patient. One to two biopsies from macroscopically benign rectal mucosa were also obtained from each patient by inserting biopsy forceps through the rigid sigmoidoscope. The biopsy samples were immediately stored in cryo vials, de-identified (blinded), labeled, and put on dry ice. The samples were stored at −70°C until shipped overnight, on dry ice, to the Massachusetts General Hospital for spectroscopy and quantitative histopathology analyses.
Spectral analysis was performed on an Avance spectrometer (Bruker, Billerica, MA) operating at 600 MHz (14.1 T). Tissue samples were placed in a 4-mm rotor with plastic inserts creating a spherical sample space of ~10 ml located at the center of the detection coil. Approximately 2.0 µl of D2O was added into the rotor with the tissue sample for field locking. All spectroscopy measurements were carried out at 4°C for tissue preservation. A repetition time of five seconds with 32 transients was used to acquire each spectrum. Spectra were collected with spinning rates of 3600 Hz. A rotor-synchronized Carr–Purcell–Meiboom–Gill (CPMG) filter (10 ms) was included in the pulse sequence to reduce broad resonances associated with probe and rotor background or macromolecules.
Spectroscopic data were processed with commercial software (Nuts, Acorn NMR Livermore, CA). All free induction decays were subjected to 1-Hz apodization before Fourier transformation, baseline correction, and phase adjustments of both zero and first order. Resonance intensities reported here represent integrals of curve-fittings with Lorentzian-Gaussian line shapes. Each sample produced well-resolved spectra with more than 50 quantifiable metabolites or regions. The 25 most intense resonance peaks were normalized by the total spectral intensity of the metabolite region up-field from water resonance (0.5~4.5 ppm), and then selected for further statistical analyses. All spectra were processed manually and objectively, without knowledge of tissue pathologic information.
After spectroscopic measurements were made, the samples were formalin fixed, embedded in paraffin, cut into 5-µm sections, and stained with hematoxylin and eosin. Sets of serial sections cut 150 µm apart were obtained from each sample. Percentages of the major pathologic components (cancer, benign epithelium, stroma, and inflammation) were visually estimated by a pathologist, and percentage of volume was calculated using a BX41 Microscope Imaging System (Olympus, Melville, NY), in conjunction with the image analyzer MicroSuite (Soft Imaging System, Lakewood, CO), as previously described.8
Principle component analysis (PCA) was used to test the hypothesis that different pathologic components have differing metabolite profiles. This method was used to identify spectral metabolite profiles that correlated with pathologic features of the tissue, such as percentages of volume consisting of malignant cells, benign epithelium, stromal cells, and inflammation. An illustration of the PCA process can be found in Figure 1. The metabolite matrix was simplified such that 25 spectral regions with intensity means above the median determined from means calculated for all regions were subjected to PCA with statistical software JMP®(SAS Institute, Cary, NC). Principle component analysis identifies combinations of metabolites whose standardized concentrations (the difference between the particular intensity of the concerned metabolite for a particular sample and the mean intensity normalized by the standard deviation, both calculated for the metabolite from the entire sample set) are indicative of changes in the matrix that likely result from pathologic differences present among the analyzed samples. The principle components (PCs) consist of individual loading factors for each spectral region that are unique to the principle component and that indicate the extent to which the metabolites within that region are associated with the process described by the principle component. Thus, principle components are independent of one another.
The first seven principle components (with eigenvalues >1.0) were subjected to paired Student's t tests to investigate the ability of each component to differentiate malignant from benign samples. Simple linear regression analysis was used to test for correlations, with principle component scores as explanatory variables and pathologic features (volume percentage of malignant cells, benign epithelium, stromal cells, or inflammation) as response variables.
In this pilot study, a total of 23 samples were collected from five patients and analyzed. The results of histopathologic examination are shown in Table 1. Macroscopically, 14 samples were judged malignant and 9 benign. Histologic examination showed that samples from four patients (cases 2–5) had been correctly categorized on the basis of macroscopy as being malignant tissue or benign mucosa; samples deriving from suspected cancerous tissue from one patient (case 1) did not contain malignant cells.
Comparison of malignant vs. benign tissue samples showed significant differences for the three principle components, as listed in Table 2, together with their eigenvalues, percentages of variances represented, and cumulative percentages of variances. In addition to this binary differentiating capacity, linear regression analysis showed several principle components had predictive value, summarized in Table 3. For instance, PC 2, accounted for 16.1 percent of the variance among the samples, correlated negatively with volume percentage of malignant tissue (P=0.0065, slope=−0.0966, Pearson's Correlation coefficient r=−0.550) and positively with volume percentage of normal epithelium (P=0.0051, slope=0.0367, and r=0.564)‥
The major loading factors for the regions that most significantly correlated with variations described by the principle components are shown in Table 4. For instance, PC 2 reflected changes in several metabolites with known connections to oncologic developments, including choline and phosphocholine.10 Metabolic activity, including choline metabolites in the spectral region from 3.20 to 3.24 ppm, which significantly contributed to the differentiation of the normal from cancerous profile established by PC 2, is shown for one pair of samples (tumor and benign from patient 3) in Figure 2.
As indicated by Table 4, the most significant loading factors of each principle component, i.e., those with larger "+" or "-" values, present the positive or negative contributions of the concerned metabolites represented by their corresponding standardized concentration. For example, if the linear regression fit for a principle component with cancer is negative (slope<0), a negative loading factor for the choline metabolites in the 3.20- to 3.24-ppm region suggests that elevation of these metabolites above the calculated mean from all samples, and the reduction of metabolites in the 3.63–3.65 ppm region from their mean, contributes to the malignant profile, as seen with PC 2 in Table 4.
The HRMAS 1HMRS technique is an advanced metabolomics methodology. Metabolomics is the science of examining the unique chemical fingerprint of a cell, tissue, or organ by measuring the global variation of metabolites present (the metabolome). The metabolome may represent dynamic and physiologic changes that take place at the cellular level and that manifest themselves as changes in metabolite concentrations before pathologic changes can be seen on a static histologic slide. In this way, metabolomics offers a means by which malignant potential may be quantified before the malignancy changes the cellular architecture. This hypothesis is the basis of what can be described as oncologic metabolomics. The HRMAS 1HMRS technique was developed specifically for the analysis of intact, unaltered tissue samples. This deviates from classical NMR of aqueous solutions and other magnetic resonance techniques, which require that metabolites be extracted from the tissue of interest, destroying the tissue and precluding histopathologic analysis. The histopathology is crucial, however, as it allows correlation of the specific metabolite profiles with the characteristics of interest (in this study, the presence or absence of malignant cells). The utility of the HRMAS 1HMRS method is further increased by its ability to be automated and free from issues of inter-observer reproducibility.
Currently, histopathologic examination can reliably differentiate benign from malignant tissues for clinical diagnosis and was the standard used in this pilot study. We used histopathologic validation to determine whether metabolite profiles for human rectal adenocarcinoma biopsy samples constructed with HRMAS 1HMRS in the same manner as that created for various other tissues might have the potential to improve the current state of radiology for noninvasive in vivo rectal adenocarcinoma diagnosis. Each of the four pathologic features examined (malignant cells, benign epithelium, stromal cells, and inflammation) manifested different biochemical changes that could be significantly delineated by PCA. The principle components that correlated with volume percentages of tumor or benign mucosa were most noteworthy.
Interestingly different principle components that correlated with the same process could have metabolite regions that contributed to the process in the same manner but with differing magnitudes, or they could have metabolite regions that contributed in different ways. For example, increases in metabolites in the 3.41- to 3.47-ppm region were related to the malignant condition for both PC 2 and PC6). However, metabolites in the 3.66- to 3.68-ppm region significantly contributed to PCs 2 and 6, but in opposite ways (diminished in the malignant condition in PC2, but elevated in the malignant condition in PC6).
The sensitivity of the current profiles may be limited by the mixed pathological features in the analyzed samples. By incorporating a larger cohort of samples and additional features, the metabolite profiles might be further refined.
Results from the current study are encouraging because they show that metabolic differences between samples containing different pathologic features can be significant enough to be measurable with HRMAS 1HMRS. Such results have important implications for future research, particularly if additional features such as tumor grade or aggressiveness are found to manifest themselves in measurable metabolic differences. If further investigations are able to correlate clinical features with metabolic profiles, HRMAS 1HMRS may be very useful for rectal adenocarcinoma, and may provide information that current in vivo radiologic means are unable to produce. One such factor of paramount clinical importance is tumor virulence, as defined by the likelihood of recurrence following curative surgery. Our examinations of human prostate cancer indicate metabolic profiles can subdivide similarly staged malignancies into groups according to aggressiveness of the primary tumor.8 Although the current study, which was at the pilot level and therefore recruited only 5 subjects, is by no means an indication that such segregation of rectal tumors is possible with HRMAS 1HMRS, it does suggest that future examinations toward this end are a valid undertaking. Because our subjects were recruited before treatment, the clinical outcomes are not known at this time and cannot be correlated with the metabolic information gathered.
Furthermore, given the potential of MRS-based metabolomic profiles in differentiating malignancy from benign tissues from the same patients, the ever increasing developments in the in vivo MR technologies may make it possible to use the present ex vivo techniques in vivo, and to inspire further technology developments targeted at investigations of in vivo noninvasive MRS in the detection and characterization of human rectal adenocarcinomas.
As an exploratory study designed to test of the efficacy of the HRMAS 1HMRS method in metabolically characterizing human rectal adenocarcinomas, the current results are significant and encouraging. The results indicate that magnetic resonance may be useful in the ex vivo biochemically classification of tissue samples as malignant or benign. In addition to the possibility of future in vivo use, further studies may reveal whether HRMAS 1HMRS, can accurately stage rectal tumors or indicate additional prognostic factors, such as a subset of more aggressive tumors that should be monitored postsurgery for recurrence.
Supported by grants from the National Institutes of Health (CA115746, CA095624), and the Massachusetts General Hospital A. A. Martinos Center for Biomedical Imaging.
Read at the meeting of the International Society for Magnetic Resonance in Medicine, Toronto, Ontario, Canada, May 3 to 9, 2008.
Reprints are not available.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text:
GCP violations and Site Mistakes commonly found During a FDA Investigator Site Inspection/Audit and how to avoid them
Product ID: 701632
- Duration: 75 Min
Customer Care
Fax: +1-650-565-8542
Email: firstname.lastname@example.com
This GCP training will guide you through key component identification of a good GCP audit program, Investigator awareness, Protocol adherence, The Informed Consent process, Evaluation of subject selection, Inclusion/exclusion criteria, data sampling and audit findings assessments.
Why Should You Attend:
All CRO's, Sites, and Sponsors of Clinical Research involved in the drug and device development process have an interest in being prepared for an audit. The main principle is to follow exactly the signed and approved (IRB + PI) protocol to the letter. This, in fact, is included on the FDA Form 1572. In the current regulatory climate, GCP Investigator site audits are a part of the clinical trial process. Sponsors should anticipate more inspections and information requests regarding monitoring practices. Many monitoring systems lack components that ensure proper management of the research site. All sites conducting Clinical Research should know how to ensure control of investigational product, data integrity, oversight of investigators as well as other areas.
Attending this webinar is a good step toward learning the FDA processes and why a knowledgeable PI is so important. The components of a quality in-house monitoring system will be presented so that participants can assess their current practices for identifying gaps and risks, particularly in relation to preparing for regulatory inspections.
It will be important to see what the FDA regulations and the ICH GCP recommendations are in this regard. The key is that the PI and all clinical staff know and follow the research plan (Protocol) exactly as it is written. This webinar will review FDA inspection program details and how to ensure your site is prepared for an inspection. It will help you to avoid the common GCP mistakes and enhance the overall audit experience.
Areas Covered in the Webinar:
- What does the FDA look at when auditing/inspecting a study?
- The regulatory requirements for the BIMO program
- Studies targeted for auditing and what does the Inspector/Auditor look for?
- What are the common GCP mistakes sites make?
- The nature of adequate oversight of all staff and non-staff
- The importance of protocol knowledge in preventing errors
- How do sites prepare for an audit / inspection
- What happens after an inspection / audit is done?
Who Will Benefit:
This Webinar will provide invaluable assistance to all personnel in the pharmaceutical, biotechnology, and CRO industry conducting clinical trials including:
- Sponsor Senior Management
- Project Managers
- CRA Managers
- QA/Compliance Personnel
- Principal Investigators and Sub Investigators
- Clinical Research Scientists (PKs, Biostatisticians)
- Safety Nurses
- Clinical Research Associates (CRAs) and Coordinators (CRCs)
- Recruiting Staff
- QA / QC Auditors and Staff
- Clinical Research Data Managers
Charles H. Pierce, MD, PhD, FCP, CPI has 20+ years' experience in the Clinical Research field. He now brings the message (via publications, webinars, lectures and seminars) of risk management through knowledge of GCP Regulations and Investigator Responsibility to the entire investigative team to help them understand the regulations as well as the ethics of research involving human subjects. He has been involved in developing Phase I & IIa Clinical Pharmacology Units, Investigator and staff GCP training, and medical monitoring in both drug and device studies.
Charles is a Fellow and Life Member of the American Academy of Family Physicians and Emeritus fellow of the American College of Clinical Pharmacology (ACCP) of which he was a Regent 1995-2000, Chairman of the 2001 annual meeting, and the 2004 recipient of the Nathaniel Kwit Distinguished Service Award. He is an active member of the Association of Clinical Pharmacology Units (ACPU) of which he was a trustee (2007-2011) and chaired the successful 2010 annual meeting. He is also a member of the Academy of Physicians in Clinical Research (APCR) and the 2012 Chair of the Board to trustees of the Academy of the Association of Clinical Research Professionals (ACRP). He is on the editorial board of the Journal of Clinical Pharmacology (JCP) and the Script Clinical Research (formerly the Good Clinical Practice Journal (GCPj)). He is on the Board of Directors of the Annapolis Center for Science-Based Public Policy.
Presently he is a Medical Director (Sub-investigator) with the Metabolic and Atherosclerotic Research Center (MARC) of Medpace. He is also President of the virtual CRO Global Clinical Partners, Inc and Pierce One Consulting.
More Trainings by Expert
Investigator responsibility/Legal commitment in drug and device clinical research: the reality
Data and Safety Monitoring in Clinical Research Involving Human Subjects - When and How
The Secret of Study Conduct Documentation to ensure a successful Audit: What the FDA Inspectors looks for
Principal Investigator responsibility in Research Involving Human Subjects: The International Conference on Harmonization (ICH) view
What is the role of Data and Safety Monitoring in Clinical Research Involving Human Subjects - When and How?
Refund Policy
Registrants may cancel up to two working days prior to the course start date and will receive a letter of credit to be used towards a future course up to one year from date of issuance. ComplianceOnline would process/provide refund if the Live Webinar has been cancelled. The attendee could choose between the recorded version of the webinar or refund for any cancelled webinar. Refunds will not be given to participants who do not show up for the webinar. On-Demand Recordings can be requested in exchange.
Webinar may be cancelled due to lack of enrolment or unavoidable factors. Registrants will be notified 24hours in advance if a cancellation occurs. Substitutions can happen any time.
If you have any concern about the content of the webinar and not satisfied please contact us at below email or by call mentioning your feedback for resolution of the matter.
We respect feedback/opinions of our customers which enables us to improve our products and services. To contact us please email firstname.lastname@example.com call +1-888-717-2436 (Toll Free).
Product Reviews
This product hasn't received any reviews yet. Be the first to review this product! Write review
Pharmaceutical Compressed Air - Quality GMP Standards and RequirementsBy: Roger Cowan
Price: $249
How to Conduct a HIPAA Risk AssessmentBy: Brian Tuttle
Price: $249
Building a Vendor Qualification Program for FDA Regulated IndustriesBy: Jonathan M Lewis
Price: $249
FDA Regulations for Marketing OTC Drugs in the U.S.By: Norma Skolnik
Price: $249
Effective Annual U.S. FDA CGMP TrainingBy: John E Lincoln
Price: $249
CDISC Mapping 1: Specifications and FDA RequirementsBy: Sunil Gupta
Price: $249
Extractables and Leachables in Early Phase DevelopmentBy: Wayland Rushing
Price: $279
Veterinary Medicine and the DEA Due Diligence Requirement for Dispensing and Prescribing...By: Carlos Aquinos
Price: $229
1099 & W-9 Update - Complying with IRS Information Reporting GuidelinesBy: Miles Hutchinson
Price: $149
OSHA Recordkeeping for 2015: Beyond the OSHA 300 SummaryBy: David A. Casavant
Price: $149
Form I-9 and E-Verify, getting and staying compliantBy: Cathleen Hampton
Price: $149
Affordable Care Act Updates: What Employers need to do to PrepareBy: Vanessa G Nelson
Price: $149
Child Support: What Payroll Doesn't Know Can Hurt YouBy: Vicki M. Lambert
Price: $229
Detecting and Preventing Embezzlement in Your OrganizationBy: Peter Goldmann
Price: $249
What Employers Need to Know About Severance AgreementsBy: Janette Levey Frisch
Price: $229
FDA's Regulation of Regenerative Medicine including Stem Cell Treatments, Tissue...
Newark, NJ | Oct 19-20, 2017
FDA's New Import Program for 2017 - Strict Precision: 2-Day In-Person Seminar by Ex-FDA Official
Philadelphia, PA | Aug 24-25, 2017
Navigating the Maze for Post-Market Compliance -- Complaint Handling, MDRs, Recalls...
Minneapolis, MN | Oct 19-20, 2017
21 CFR Part 11 Compliance for SaaS/Cloud Applications: 2-day In-person Seminar
Philadelphia, PA | Sep 14-15, 2017
Quality Control Laboratory Compliance - cGMPs and GLPs: One and a Half-day In-person Seminar
San Francisco, CA | Nov 9-10, 2017
Effective Quality Oversight of Pharmaceutical Contract Manufacturing Organizati...
San Francisco, CA | Jul 27-28, 2017
Latin America: Regulatory Compliance Requirements for Life Science Products...
Philadelphia, PA | Aug 24-25, 2017
Computer System Validation - Reduce Costs and Avoid 483s: 2-Day In-Person Seminar
Philadelphia, PA | Sep 14-15, 2017
Complete 1099, TIN Matching, B-Notice, FATCA and Nonresident Alien 1042-S Compl...
San Francisco, CA | Jul 27-28, 2017
Ensuring Compliance with Advertising and Promotional Requirements for Drugs and...
Boston, MA | Nov 9-10, 2017
Managing Your FDA Inspection: Before, During and After: 2-Day In-Person Seminar by Ex-FDA Official
Tampa, FL | Aug 17-18, 2017
ACH Risk Management: 2-day Workshop
Boston, MA | Jul 20-21, 2017
Metrics & Predictive Analytics in Human Resources-Reducing Risk and Improving...
Washington, DC | Sep 14-15, 2017
Designing and Sustaining New and Existing Product Stability Testing Program
San Diego, CA | Sep 4-5, 2017
Managing an Effective AML Compliance Program: 2-day In-Person Seminar
Chicago, IL | July 20-21, 2017
General requirements for the competence of testing and calibration laboratoriesProvider: ANSI
Price: $142
IEC 62304:2015 ''Medical Device Software - Software Life Cycle Processes''Provider: SEPT
Price: $299
IEC 62304:2015 ''Medical Device Software - Software Life Cycle Processes''Provider: SEPT
Price: $149
ISO 13485 Procedure 754-Customer PropertyProvider: 13485 Store
Price: $12
Measurement management systems - Requirements for measurement processes and measuring equipmentProvider: ANSI
Price: $120
Geometrical Product Specifications (GPS) - Geometrical tolerancing - Tolerances of form, orientation...Provider: ANSI
Price: $250
Templates and Plans for Software Configuration Management Documents-Version 6.0Provider: SEPT
Price: $299
Computer System Validation Master PlanProvider: LabCompliance
Price: $90
Zero Acceptance Number Sampling Plan for Receiving, In-Process and Final InspectionProvider: Quality-Control-Plan
Price: $37
Standard Operating Procedure: FDA InspectionsProvider: LabCompliance
Price: $49
AS9003 Comprehensive Quality System Kit for Inspection and Test Quality Control SystemProvider: At-PQC
Price: $397
ISO 13485 and FDA Compliant Internal Audit Tools: Checklist, Procedure and FormsProvider: 13485 Store
Price: $95
Template for a Software Maintenance Plan - Fourth EditionProvider: SEPT
Price: $99
Sarbanes Oxley Treasury Risks and ControlsProvider: Audit Net
Price: $50
ISO 13485 Gap ChecklistProvider: 13485 Store
Price: $59


text:
Look at other dictionaries:
intrinsical — index central (essential), essential (inherent), organic Burton s Legal Thesaurus. William C. Burton. 2006 … Law dictionary
intrinsical — adjective Date: circa 1548 archaic intrinsic … New Collegiate Dictionary
intrinsical — adjective intrinsic … Wiktionary
intrinsical — adj. essential, fundamental; inner, internal; personal; real (also intrinsic) … English contemporary dictionary
intrinsical — a. See intrinsic … New dictionary of synonyms
intrinsical — in·trin·si·cal … English syllables
intrinsical — adjective belonging to a thing by its very nature form was treated as something intrinsic, as the very essence of the thing John Dewey • Syn: ↑intrinsic • Ant: ↑extrinsic (for: ↑intrinsic) • Similar to … Useful english dictionary
intrinsecal — Intrinsical In*trin sic*al, a. [Formerly written {intrinsecal}.] [1913 Webster] 1. Intrinsic. [1913 Webster] 2. Intimate; closely familiar. [Obs.] Sir H. Wotton. [1913 Webster] … The Collaborative International Dictionary of English
Tait-Bryan rotations — The Tait Bryan rotations, named after Peter Guthrie Tait and George Bryan. are three elemental rotations about each one of the principal axes of a body (but not necessarily the principal axes of inertia). For a craft moving in the positive x… … Wikipedia
Intrinsicalness — In*trin sic*al*ness, n. The quality of being intrinsical; intrinsicality. [1913 Webster] … The Collaborative International Dictionary of English


text:
Need direct lab tests in Hewitt, TX? Simply order direct lab tests online and get tested at a lab in Hewitt, TX (Find More Locations):
Why would I get a Direct Labs Test?
A Direct Labs Test is a process by which people can order labs directly online. People in Hewitt, TX order a Direct Labs Test for general screening or to track their health over time with lab testing.
How much does a Direct Labs Test cost?
Our prices to order a Direct Labs Test in Hewitt, TX start at under $50. To get started, please CLICK HERE.
What does a test report look like?
For a sample report of a popular panel available at our Direct Labs Test in Hewitt, TX, please CLICK HERE.
We are delighted to offer a Direct Labs Test in Hewitt, TX.
Direct Labs Test in other cities near Hewitt, TX:Bruceville | Chilton | China Spring | Crawford | Eddy | Elm Mott | Leroy | Lorena | Mc Gregor | Riesel | Ross | Satin | Waco | Woodway


text:
Pink Kong Studios is a Dublin based animation studio bringing stories to life.
Pink Kong Studios is an award winning animation studio creating multiplatform brands for Children's entertainment. The Dublin based company grew from a vision to create exciting properties with a particular focus on action adventure brands for girls. Co-founded in 2014 by Aoífe Doyle and Niamh Herrity, all our properties are created with a multiplatform end user in mind from the outset. Our prime audience is the US, UK & European broadcasters and content distributors. Buyers who are looking for "game changing" content and as a fresh new take on storytelling.
Urban Tails, Rova-Novas, Action Ailbhe
- Bank of Ireland Start Up Awards, National Nomination 2016
- Winner of the LEO Dublin City Women in Business Network 2015 'High Potential Business' Award,
- Winner of 1minuto film festival August 2015


text:
Posted by Tom
I worked with Lisa Green on several very difficult cases. She was really, really great to work with, and very responmsive. I am a person who likes to get involved in any litigation I am involved in, and Lisa really understood the issues, and provided us with a very well thought out defense. Her discovery skills were superb, and the outcome of both cases were very good for both my company and my professional liability carrier. I would easily recommend Lisa Green to other potential clients.


text:
Stick to Your New Year's Health Resolution with these Tips
Sticking to your New Year's health resolutions can often be a challenge. Finding the motivation as well as the willpower to continue going can often be hard however there are ways to fix this. With the help of Mannatech's All About Mannatech blog, we were able to come up with five simple ways to help you stick to your New Year's Health resolution and become an even better version of yourself.
Health Tip: Fighting Stress
When you're super stressed, exercise is a great way to blow off steam, do a good deed for your body and distract yourself.
The Five Best Health Boosts for Women
Every woman who wants to live a healthy lifestyle must ensure that she taking proper supplements. Head to a supplement provider like Mannatech where you can purchase amazing health supplements for women or take a look at the list below. You must ensure that you have researched each supplement and consulted a health professional before you begin a regimen, and you may consider speaking to a doctor first.
Recent Posts
- Understanding different causes of drug shortages June 20, 2017
- Cleveland Clinic and the UAE Joined Together to Donate Soccer Field June 16, 2017
- Physician Elected President of AAAS May 2, 2017
- Stick to Your New Year's Health Resolution with these Tips January 24, 2017
- Sports Supplements aren't just for Athletes March 31, 2016


text:
01782 223822
You've already added this type of sample to
your basket, heres some other suggestions we
think you might like...
You've already added this type of sample to your basket..
Completely Free Tile Samples
Totally free cut samples dispatched same day!
5 Star Trustpilot Reviews
Scoring higher than Topps Tiles & B&Q
Visit Our Flagship Showroom
Open 7 days a week in Stoke-on-Trent
Free Next Day Delivery*
On orders over £150*
Items 1 to 5 of 23 total
"Our pallets are delivered to the kerbside.Watch the video to find out more."
In Stock
Clear stain protector for natural stone, marble, granite and polished porcelain.This product will cover approximately 7.5m2.
Fila MP90 Datasheet
With 250ml (based on single coat):
- Stone: Approx 7.5sqm
- Polished porcelain: Approx 7.5 - 10sqm
You've already added this type of sample to your basket..
Amount required:
Add these extras for:
Clear stain protector for natural stone, marble, granite and polished porcelain. This product will cover approximately 7.5m2.
*to most mainland UK postcodes
Q : Can I use this to seal the white grout too?
If you were planning on using the Fila MP90 sealer to seal your polished tiles, it can also be applied over the grout joints to seal the grout. However, if you were looking for a product to seal just the grout, we would recommend using the Fila Fuga Proof Grout Protector (SKU: 430680).
Q : Craquele tiles fixed to kitchen walls. Should Fila MP90 Polished Porcelain & Stone Sealer be used before or after grouting ?
We would recommend sealing the tiles prior to installation to avoid any staining from the grout and again after installation and grouting.
Q : hi is it advisable to apply sealer before or after grouting,i am going to use on ceramic craquele wall tiles thank you
Q : i recently purchased the Brix anthracite wall tiles from you and Roma dark grey floor tiles. Should i use this product on them?
The Brix anthracite and the Roma Dark Grey tiles do not require sealing after installation.
Q : We bought your polished porcelain tiles and also this FILA sealer. Unfortunately, with builders and tradesmen coming and going we haven't had a 24 hour window to apply this. Is it ok to apply this a few weeks after the tiles have been layed. Do I need to clean them with a particular product first?
You are able to apply the sealer later, however, you will need to make sure that they have been thoroughly cleaned first. If there are any residues left over, we would recommend to use our Fila Deterdek Residue remover (SKU: 430650) before applying the sealer.
Items 1 to 5 of 8 total
ordered free sample tiles which arrived very quickly and well packaged. Unfortunately the colour was unsuitable but I received a call and email offering to send others free of charge. Excellent servic... Read More
MICHELLE July
Number one online for tiles!
© 2015 --> 2017 Tile Mountain Ltd.
Please wait...


text:
Skip to content
Search for:
West Coast MLB
AL & NL West MLB Coverage
American League West
Houston Astros
Los Angeles Angels
Oakland Athletics
Seattle Mariners
Texas Rangers
National League West
Arizona Diamondbacks
Colorado Rockies
Los Angeles Dodgers
San Diego Padres
San Francisco Giants
MLB News
WestCoastMLB Prospects
Fantasy Baseball
World Baseball Classic
MLB Featured Videos
MLB The Show (Video Game)
Contact Us
Privacy Policy
Tag: red sox
May 4
Curt Schilling The Idiot On Adam Jones: "I Don't Believe His Story, Social Justice Warrior."
May 3
-2004- Barry Bonds On Playing In Boston! "Too Racist For Me, Would Never Play There."
Apr 25
Machado vs Pedroia! Red Sox vs Orioles! Makings Of New AL East Rivalry?
Apr 4
Andrew Benintendi : Boston Red Sox Rookie Outfielder Looks Like The Real Deal!
Mar 25
Mar 26
Mookie Betts Of The Boston Red Sox Is The New Face Of Baseball! (#3 WAR 7.8)


text:
Evolutionary changes in the notochord genetic toolkit: a comparative analysis of notochord genes in the ascidian
The notochord is a defining feature of the chordate clade, and invertebrate chordates, such as tunicates, are uniquely suited for studies of this structure. Here we used a well-characterized set of 50 notochord genes known to be targets of the notochord-specific Brachyury transcription factor in one tunicate,
Of the 50
A surprisingly high number of
The notochord is the synapomorphy that gives chordates their name. In vertebrates, this embryonic structure is transient, as it is replaced by the vertebral column, and acts as a powerful organizer, secreting signals required for the patterning of several organs, such as the floor plate, somites, pancreas, heart and dorsal aorta
While these observations indicate that ascidians represent a valid model for studying the evolutionary origins of the notochord, they also raise questions on the applicability of these findings to other invertebrate chordates. In fact, a considerable degree of variability in the structure and genetic makeup of the notochord has been found among invertebrate chordates; the most striking example of such variability is arguably the notochord of amphioxus (cephalochordate), which is functionally related to the ascidian notochord but expresses numerous muscle genes
In this study, we have begun probing the extent of variation tolerable in the notochord gene complement used by representatives of two classes of tunicates. To this aim, we tested in the larvacean
Figure 1
The notochord of
The notochord of
The conserved notochord expression of
Results and Discussion
Lineage-specific duplication events among
To identify notochord genes in
Additional file 1
Table 1. Ci-Bra target notochord genes and their
Click here for file
For each reciprocal best BLAST hit, the corresponding
Additional file 2
Figure S1. Protein sequences used for phylogenetic reconstructions.
Click here for file
To construct a phylogenetic tree of orthologs of
Figure 2
Phylogenetic analyses of
Phylogenetic analyses of
Three copies of the
We used the reciprocal best BLAST hit method to identify orthologs of
Gene duplications, synteny and genomic rearrangements: the case of the
Another phylogenetic tree was constructed to study the evolutionary history and reciprocal relationships of
Figure 3
Phylogenetic analyses and genomic organization of
Phylogenetic analyses and genomic organization of
Identification of novel
Specific cDNA fragments corresponding to 28
Additional file 3
Table 2. Sequences of the oligonucleotides used as primers in the PCR amplifications.
Click here for file
Additional file 4
Table 3. Summary of the
Click here for file
Figure 4
Identification of novel notochord genes in
Identification of novel notochord genes in
Figure 5
Uneven expression of
Uneven expression of
Figure 6
Figure 7
We found that conserved
Additional file 5
Figure S2. Whole-mount
Click here for file
Additional file 6
Figure S3. Additional expression patterns in
Click here for file
While little is known about the possible role of the putative non-muscle myosin heavy chain encoded by
Among the
A discontinuous pattern similar to the pattern that we observed for
The functional meaning and developmental consequences of a discontinuous source of Netrin in the notochord remain to be elucidated. However, it is intriguing that the
Another peculiar expression pattern has been revealed by this study for
Seven of the
Six of the
Considering the morphological similarities between the notochords of
Figure 8
Conservation of
Conservation of
It has been demonstrated by previous studies that extracellular matrix proteins are crucial for notochordal integrity
First insights into the
A comparison between the expression patterns of the
Most of the notochord genes identified in this study are expressed in the notochord at mid-tailbud and Stage 1; however, in Stage 2 embryos expression of several of these genes begins to fade in notochord cells, and in older embryos all these genes become no longer detectable. This suggests that the temporal regulation of the transcription of these genes is differentially modulated in
Tunicate embryos provide excellent model systems for studies of notochord development and evolution. Here we have compared the molecular toolkits of the simple notochords of two divergent tunicates,
The results of the expression studies presented here suggest that the gene duplications that we have uncovered may have included to a variable extent the
In higher chordates, the notochord is known to serve a dual function: providing structural support to the developing embryo, and secreting patterning signals required for tissue specification and organogenesis
These observations, together with the remarkable absence from the
Animal collection and culture
Identification of
Additional sequences from chordates of interest were retrieved through the BLASTX, TBLASTN and BLASTP algorithms using the respective genome and/or ESTs browsers. The reciprocal best BLAST hit method was used to select putative orthologous sequences. Whenever necessary, existing gene models were individually verified by aligning genomic and EST sequences using the BioEdit sequence alignment editor
Phylogenetic analyses
Multiple alignments of deuterostomes sequences were performed using the MUSCLE 3.6 software and modified manually whenever necessary
Probe Preparation
All probe templates were amplified by RT-PCR as previously described
Embryos were fixed in 4% paraformaldehyde, 0.1 M MOPS (pH 7.5), and 0.5 M sodium chloride overnight at 4°C, then washed in 0.1 M MOPS (pH 7.5) and 0.5 M sodium chloride, and stored in 70% ethanol at -20°C until use. Hybridization and detection of probes were performed as in
bp: base pair(s); cDNA: complementary DNA; EST: Expressed Sequence Tag; hpf: hour(s) post-fertilization; kb: kilobase(s), or 1000 base pairs; Mb: megabase(s); MOPS: 3-(N-morpholino) propanesulfonic acid; PCR: Polymerase Chain Reaction; RT: reverse transcriptase; SSC: saline-sodium citrate solution; tRNA: transfer RNA; WMISH: whole-mount
Authors' contributions
JEK performed BLAST searches, cloned and synthesized probes for WMISH, performed WMISH, and drafted portions of the manuscript. PK performed BLAST searches, carried out the phylogenetic analyses, cloned and synthesized probes for WMISH, performed WMISH, and drafted portions of the manuscript. J-MB oversaw the
We are grateful to Dr. Daniel Chourrout for his invaluable help with
Notochord patterning of the endoderm
Structure and function of the notochord: an essential organ for chordate development
Gene expression profiles in
Ascidian notochord morphogenesis
Regulation of NF-kappaB/Rel by IkappaB is essential for ascidian notochord formation
Direct activation by Ets and Zic is required for initial expression of the
Development of the appendicularian
Characterization of a notochord-specific enhancer from the
The regulation of
The evolutionarily conserved
Evolutionary conservation of vertebrate notochord genes in the ascidian
Direct activation of a notochord
Conservation of notochord gene expression across chordates: insights from the
Genes expressed in the amphioxus notochord revealed by EST analysis
Expression of muscle-related genes and two
Conservation of
Characterisation of amphioxus
A signalling relay involving Nodal and Delta ligands acts during secondary notochord induction in
Unfolding a chordate developmental program, one cell at a time: invariant cell lineages, short-range inductions and evolutionary plasticity in ascidians
Miniature genome in the marine chordate
Culture optimization for the emergent zooplanktonic model organism
Embryology of a planktonic tunicate reveals traces of sessility
Developmental biology of ascidians
Generation of
Continued growth and cell proliferation into adulthood in the notochord of the appendicularian
Brachyury-downstream gene sets in a chordate,
Regulation of
Predicting function: from genes to genomes and back
A genomic perspective on protein families
Plasticity of animal genome architecture unmasked by rapid evolution of a pelagic tunicate
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Improved genome assembly and evidence-based global gene model set for the chordate
Rho-family GTPases: it's not only Rac and Rho (and I like it)
The far upstream element-binding proteins comprise an ancient family of single-strand DNA-binding transactivators
Messenger-RNA-binding proteins and the messages they carry
The CREC family, a novel family of multiple EF-hand, low-affinity Ca(2+)-binding proteins localised to the secretory pathway of mammalian cells
Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in
Spatiotemporal patterns of neurogenesis in the appendicularian
Zebrafish mutants identify an essential role for laminins in notochord formation
Ascidian prickle regulates both mediolateral and anterior-posterior cell polarity of notochord cells
Brachyury-downstream notochord genes and convergent extension in
Organizing the cell cortex: the role of ERM proteins
The KH domain protein encoded by
Localization of two IQGAPs in cultured cells and early embryos of
Netrins are diffusible chemotropic factors for commissural axons in the embryonic spinal cord
Characterization of Brachyury-downstream notochord genes in the
Lineage-independent mosaic expression and regulation of the
An amphioxus
Scanning electron microscope observations on the muscle innervation of
Development of the caudal nerve cord, motoneurons, and muscle innervation in the appendicularian urochordate
Brachyury downstream notochord differentiation in the ascidian embryo
Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in
Molecular evolution of fibrillar collagen in chordates, with implications for the evolution of vertebrate skeletons and chordate phylogeny
Development and evolution of chordate cartilage
Analyzing gene regulation in ascidian embryos: new tools for new perspectives
Development of a chordate anterior-posterior axis without classical retinoic acid signaling
Evolutionary sequence analysis of complete eukaryote genomes
The draft genome of
Expression of
BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT
MUSCLE: multiple sequence alignment with high accuracy and high throughput
BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0
Phylogeny.fr: robust phylogenetic analysis for the non-specialist
MRBAYES: Bayesian inference of phylogenetic trees
MrBayes 3: Bayesian phylogenetic inference under mixed models
Prediction of complete gene structures in human genomic DNA
Amino acid substitution matrices from protein blocks


text:
The hotel is very beautiful. It is situated in the nature. The day after the check-in, in the morning I saw a deer and it was a magical experience. The rooms are big and vhe ery clean. The staff is very professional and helpful. The...More
Sunstar Boutique Hotel Castello di Villa


text:
I have seen several posts over the years from people who don't want the ticket to automatically assign itself to whomever makes the first comment.
I have an idea of how to accomplish this, where I think a plugin will suffice. A plugin could listen, and assign every incoming ticket to a generic "IT Department" user. Then the ticket becomes pre-assigned and won't change until told to do so. So people can make initial comments on a ticket, and it won't assign itself to them because it's already assigned to the "IT Department" user.
Ideally this plugin would create the user "IT Department" (if possible), and be configurable as to the name of that user. It would be really nice if it allowed you to re-assign the ticket without clicking the edit button first, to make that part of it easier.
I'm certain I don't have the skills to create this plugin, but I would be happy to test it.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
by Lynn
I ask all of you to help me in praying to God for help with weight loss. My self esteem is so low now and I have no willpower. I pray that God can guide me to make wiser decisions and think before eating fattening foods. Please let him guide me to eat more things with nutrition. I also ask that he help me to fight off my low energy level and help to get on the Schwinn Aerodyne that sits in my house. I need to start exercising to feel better. Thank you to all who help me and keep me in your prayers.
Join in and write your own page! It's easy to do. How? Simply click here to return to Daily Prayers.
Click for Inspiration
50 Inspirational Bible Verse Pictures
See What Happens After A Single Act Of Kindness
By The End of This Video, Your Faith Will Be Stronger Than Ever
See What Happens When Someone Pays For A Random Stranger's Groceries
Popular Prayers
Must Reads:
5 Powerful Prayers for Prosperity
Does God Answer Prayers?
8 Prayers You Need to Know by Heart
The Trick to Powerful Prayer


text:
Comedy - What do you do when the party of the year kicks you to the curb? Simple... create your own! When Sean (Franz Drameh, Attack the Block) and his friends are refused entry to the night of their year, they embark on a mission to stage a monster event, whilst at the same time taking down the opposition. - Franz Drameh, Amy Tyger, McKell David
This is Legacy and you are watching MovieZion
Please wait for 3 seconds, we are loading Legacy stream.
Please enter captcha to continue to video.
If the above stream is offline (saying We are sorry!),
please click on the "Refresh Link" to get a new streaming link. Don't worry it'll just take a second.


text:
Quilt Pink Organic Cotton Chenille BlanketKlippan
Brand: Klippan
Designed by Lotta Glave & Bengt Lindberg
Details: 100% organic cotton chenille blanket, 70cm x 90cm.
A velvet soft blanket that is perfect for pram or cot or just snuggling up. Lotta Glave's fabulous illustrations make up the squares of this quilt inspired blanket making it a wonderful present for a newborn.
Hand wash or professional dry clean. Do not tumble dry, do not wash in washing machine. Iron on low wool setting, do not use bleach.


text:
Doctor insights on:
Do People Ever Get Pregnant After A Tubal Ligation
Do people ever get pregnant after a tubal ligation?
Yes: It depends on the method used to ligate your tubes, but roughly one percent (1%) failure rate is in the ballpark for most methods currently in use. Failed tubal ligation can result not only in intrauterine pregnancy but ectopic pregnancy, which is usually tubal. ...Read moreSee 1 more doctor answer
Pregnancy (Definition)
When your due date arrives, you will be more than ready to have your baby! Most women deliver the baby somewhere between 37 and 42 weeks. According to the American College of Obstetricians and Gynecologists, only 5% of babies arrive on the exact due date. Approximately 7% of babies are not delivered by 42 weeks, and when that happens, it is referred to ...Read more
Please explain if it is possible to get pregnat after tubal ligation and 1 ovary removed?
Yes: Tubals can sometimes be reversed. You can also have IVF done where they take sperm and egg and combine them outside the uterus and place the embryo into the uterus, bypassing the tubes. If you are thinking of getting pregnant at the age of 48, you should contact a fertility specialist ASAP. ...Read moreSee 1 more doctor answer
Can i be preganant? I hda a tubal ligation 19 years ago and my cycle is 3 weeks late, I have taken two test both results were negetive
Trust the test: Home pregnancy tests are very accurate. They are the same tests used in a doctor's office. They can detect a pregnancy 7-10 days after conception. The best time to take one is when you miss your period. If you doubt the result you can repeat it in 1-2 days. If you were pregnant the hormone level would rise enough for detection. ...Read more
Tubal ligation 13 years ago bleeding one week after period feel pregnat cyst on my ovaries any advice please?
See your gyn: This could be just hormonal but tubal ligations can fail and when they do your risk for an ectopic pregnancy is high. Please see your gyn. ...Read more
I am 43 and had a tubal ligation 9 years ago and an ablation 5 years ago. I started spotting and cramping a little today. Is this normal or is pregna?
GYN exam: Given your tubal ligation and ablation, pregnancy is very doubtful. Given your age, I suggest a GYN exam, female hormone and thyroid testing, PAP smear and if needed a uterine sampling biopsy. Please do not ignore these symptoms. You may also require a transvaginal ultrasound. ...Read more
Could i get pregnant after a tubal ligation?
Very little: Most women who have a tubal sterilization will not become pregnant. The failure rate is different for the many types of procedures that are done to sterilize the woman. ...Read more
Do people get pregnant after a tubal ligation?
NO not unless: U have your tubes reversed or in vitro fertilization, a small percentage of tubals can fail in that case you are more at risk for an ectopic. ...Read more
Is it possible to get pregnant after a tubal ligation?
Yes: yes it is possible but it the likelihood of an ectopic pregnancy is higher. You can also talk to your obgyn about reversing the procedure. ...Read moreSee 1 more doctor answer
How likely am i to get pregnant after a tubal ligation?
Slim: If properly done, you should have very low chance of getting prego. ...Read more
What can you do if you get pregnant after a tubal ligation?
Options: First, it's critical to determine asap if the pregnancy is intrauterine, as pregnancy after tubal ligation is highly risky for ectopic (usually tubal) pregnancy. If ectopic, medical or surgical treatment follows; if the pregnancy is intrauterine then decision-making is identical to any other pregnancy. Following this, you could consider a second ligation or another form of permanent sterilization. ...Read moreSee 1 more doctor answer
Tubal Ligation (Definition)
Sterilization procedure for women. It may involve a small incision in in the belly botton to fulgrate the tubes or tie them and remove ...Read more
- Talk to a doctor live online for free
- What can you do if you get pregnant after a tubal ligation?
- Percentage women get pregnant after tubal ligation
- Chance of getting pregnant after tubal ligation
- Ask a doctor a question free online
- Best time to get pregnant after tubal ligation
- How to get pregnant after a tubal ligation reversal?
- Different pregnant after tubal ligation
- What are my chances of getting pregnant after a tubal ligation?
- Talk to a gynecologist online for free


text:
A Bachelor's degree is a degree awarded by universities and colleges. In order to earn a bachelor's degree, you must complete the necessary courses that may last three to seven years depending on your institution of enrollment.
Business studies is an academic program that is designed for students wanting to learn more about how businesses are run. Courses may include more general topics or more specific ones to help prepare students for a particular career.
The USA remains the world's most popular destination for international students. Universities in the US dominate the world rankings and the country also offers a wide variety of exciting study locations. State university systems are partially subsidized by state governments, and may have many campuses spread around the state, with hundreds of thousands of students.
Request Information Bachelor Degrees in Business Studies in Charleston in USA 2017
The baccalaureate degree in Natural Resource Management provides students with the knowledge, analytical and practical skills, and decision-making competencies to protect and use effectively our renewable resources. [+]


text:
'Bachelorette' Desiree Hartsock Expecting First Child
It's a bachelorette baby!
… More »
It's a bachelorette baby!
… More »
Soulja Boy's latest duet is with one of the hottest MCs in the game… Bachelorette Desiree Hartsock?
Bachelor host Chris Harrison tweeted yesterday, "An appetizer to get ya goin #BachelorNation Are these guys here for the 'right reasons'" along with a link to this video of the rapper and Bachelorette rhyming with the new season's contestants. Musical magic… More »
Meet the latest star of ABC's The Bachelorette.
Desiree Hartsock is the 26-year-old beauty from Colorado who's about to embark on her journey to find everlasting love.
The bridal stylist, who now resides in Newport Beach, Calif., tried to win the heart of Bachelor Sean Lowe, but was sent home sans a… More »
The next "Bachelorette" is here.
ABC has confirmed reports that Desiree Hartsock will be the next Bachelorette for Season 9 – which is slated to air later this summer.
The 26-year-old stylist from Colorado may have to watch out for longtime Bachelor fan Pattie Mallette (Justin Bieber's mom) for her coveted… More »
She may not have won Sean Lowe's final rose, but Desiree Hartsock will still have her shot at love.
"I just can't even believe I'm here," Hartsock, known affectionately as Des, told Chris Harrison after he announced her on Monday's After the Final Rose special.
"I could never have imagined signing up for The Bachelor and having this… More »


text:
Multi Skilled Maintenance Engineer
- Recruiter
- Future Engineering Recruitment
- Location
- Ludlow
- Salary
- £27000 - £30000 per annum + Package
- Posted
- 06 Oct 2016
- Closes
- 20 Oct 2016
- Sector
- Engineering
- Contract Type
- Permanent
Maintenance Engineer £27,000- ££30,000 Package Days Only Shift Ludlow Brilliant opportunity for a maintenance engineer with a background in manufacturing to work in a close knit team, completing routine tasks and having a positive impact on the business. Great package on offer and a job for life for the right engineer. Work for a British manufacturer in a friendly and stable environment. Perfect for experienced engineers looking for a company that recognise hard work. The Role: Reactive and planed maintenance engineering Electrical and manufacturing plant Project work Days only shift The Person: Experienced maintenance engineer Good manufacturing experience Experience of control systems Hydraulics and Pneumatics fault finding Apply now or call 02034114143 and ask for Louis Keywords: Maintenance engineer, building service, manufacturing, Ludlow, electrical, hydraulics, pneumatics, plant. This vacancy is being advertised by Future Engineering Recruitment Ltd. The services of Future Engineering Recruitment Ltd are that of an Employment Agency. Please visit our website at futureengineer.co.uk to view other positions we are currently handling. Future Engineering Recruitment Ltd can only accept applications from candidates who have a valid legal permit or right to work in the United Kingdom. Potential candidates who do not have this right or permit, or are pending an application to obtain this right or permit should not apply as your details will not be processed. We will endeavour to respond to all applicants however due to the sheer volume of response, we can only guarantee that candidates who have been shortlisted will be contacted. This job was originally posted as www.totaljobs.com/job/66812123


text:
I'm not even done watching this video yet, but I felt the need to blog it out. The Edward is different (le sniffle, 'cause I actually liked the old one), but I am dying. I swear, it's like the Hillywood Show read my fricking mind when I was thinking that "Marry The Night" would be perfect (though "Marry And Wed" just sounds funny). This parody isn't my favorite so far, but hey! I'm not even done yet. The Jacob, though...Oh my gosh, lol. Watch it!!
Have fun!
TeamTaycob 03:46, February 2, 2012 (UTC)
UPDATE: I have now seen the parody. Okay, it's not as epic as the Eclipse one, and it also seemed a bit rushed, but I still enjoyed it. Jacob. On. The. Honeymoon. LMFAO.
And now, an honest review of the parody:
Straight off the bat, I'm going to say that I like it. I don't love it, but I do like it a lot. It seems a bit short and also rushed, but the movie was also short and rushed, so the Hillywood Show didn't have a lot to work with. I know a bunch of Hillywood fans are going, "Where's Jacob?!" (as in the actor Jacob Jost, who [ironically] plays Edward). I was thinking the same thing. I love Jacob's portrayal of Edward, so I was disappointed as well. I started crying and screaming when I heard "Marry The Night" (though the "Marry And Wed" thing was weird), since I had thought earlier that the song would be great for a Hillywood Show parody. The rest of the parody was so-so. The "Having My Baby" parts were so awkward, but I liked them. Overall, I think the only thing that makes this parody decent is Jacob (as in Kyle Dayton, who plays Jacob Black). He is so random in this video, it's hilarious. I loved all his scenes. Also, he looks a bit like Taylor Lautner, which is great dedication on the Hillywood Show's part. I love the Hillywood Show, but the Breaking Dawn - Part 1 parody isn't the best. Hopefully, their Twilight parodies will go out with a bang with Breaking Dawn - Part 2.


text:
Brenners Kids-Club
Dear children,
A warm welcome to 'Brenners'. We have been a family owned hotel for 140 years, and always give a special welcome to our younger guests. As soon as you've unpacked your case, you can join in sports, games, and handicrafts or rush out into our private park for fresh air and fun.
The Kids-Club is based in a colourful room filled with soft toys, books, games and multi-media entertainment. Warm and friendly professionals are there to introduce the many programmes such as a trip to the nearby Museum Frieder Burda, to learn about famous artists, or a behind the scenes tour of our hotel.


text:
JACKSON — A project to turn the main thoroughfare in downtown Jackson into a two-way street is expected to start by year's end.
The city must have state approval for construction plans on the Capitol Street project by Sept. 30. Work needs to begin no later than January, or it will miss out on millions in federal funding, according to The Clarion-Ledger.
Ben Allen, president of Downtown Jackson Partners, said area leaders have been discussing the idea since the early 2000s.
"We're the only major city that I know of that hasn't converted their main street downtown back to two-way," he said.
Converting Capitol Street into a two-way street is part of an overall plan to make the street more attractive and accessible.
Preliminary design sketches call for additional parking, roundabouts at four intersections, new landscaping and dedicated bike lanes.
The idea, says Public Works Director Dan Gaillet, is to make the street more accessible to cars while slowing them down so pedestrians feel more comfortable. "Again, part of the goal is to make livable spaces where folks can easily travel up and down the street to shop, visit, etc."


text:
Red Prita Silk Dress
Diane von Furstenberg Red Prita Silk Dress
Product Description:
Product Description:
Bright and bold florals add autumnal color to this tailored shirtdress that features a waist-cinching tie and a slight hi-lo hem. Point collar. Long sleeves. Buttoned cuffs. Button-front closure. Pocket at chest. Self-tie belt. Gathered back yoke. Hi-lo hem. About 25" from natural waist. Silk. Dry clean. Color: floral daze
US0: Chest 90cm, Shoulder 37cm, Length 82-90cm
US2: Chest 94cm, Shoulder 38cm, Length 85-92cm
US4: Chest 98cm, Shoulder 38cm, Length 86-94cm
US6: Chest 102cm, Shoulder 39cm, Length 87-95cm
US8: Chest 104cm, Shoulder 39cm, Length 87-96cm
US10: Chest 108cm, Shoulder 40cm, Length 88-96cm
The size chart above is provided to help determine your size when ordering from us. As Sizing and cut will vary between brands, please use this information as a general to compare the various size scales. Should you be between sizes or need assistance selecting the proper size, please contact our Customer Service department by email.
Ship directly from factory, may cut or change label, but guaranteed 100% Authentic
Back To Top
Designer Styled Items are all on sale up to 80% off
Custom Search
1. FINAL SALES: All sales of gift cards, e-gift certificates, cosmetics, jewelry and items marked as "Sale" are final. FINAL SALE items cannot be returned for exchange, credit, or refunds.
2. Shipping charges are none refundable.
3. We ship worldwide. However, we only use regular shipping, NO overnight shipment.
4. To prevent fraud, your billing address must match the address to which your monthly credit card statement is sent.
5. All price are in US$
6. Shipping charges are non-refundable.
If the received goods does not show exactly the same figure on the web, just because the causes of lighting differences, we are not offer exchange or return. Most of our products are in size US2-US8, Karen Millen dresses are in UK8-UK16, make sure the size before you purchase them. We won't make exchange or return if the size is not right. However, if you are willing to pay the resent shipping fee, we'll make the exchange.
Still notice that we do not use UPS or DHL shipping, and do not send urgent package. Also we don't accept UPS or DHL returned package.


text:
Stocks edge higher but leave banks behind
NEW YORK (AP) Financial stocks fell broadly Thursday, left out of a late lift that pared earlier losses for most major stock indexes.
Goldman Sachs (GS) dropped after Sen. Carl Levin, D-Mich., said that a panel he leads has found new evidence that shows Goldman misled investors. Levin also raised doubts about the testimony given last year by Goldman executives .JPMorgan Chase & Co. and Bank of America Corp. each fell more than 1%.
The Standard & Poors 500 index was essentially unchanged at 1,314. The Dow Jones industrial average added 14 points, or 0.1%, to 12,285. The Nasdaq composite fell 1, or 0.1%, to 2,760.
Four stocks rose for every three that fell on the New York Stock Exchange. Consolidated volume came to 4 billion shares.
The three major indexes remain lower for both the week and month.
Stock trend
Dow Jones industrial average, five trading days
Until recently, most investors assumed banks had put their troubles behind them, said Todd Salamone, director of research at Schaeffers Investment Research. He pointed to JPMorgan Chase, the first large bank to release earnings. The bank reported first-quarter results Wednesday that beat forecasts, but CEO Jamie Dimon warned that the company could still take more losses from mortgages.
Thats one of the better banks, Salamone said, so it makes you wonder whats going to happen going forward.
Bank of America, the countrys biggest bank and one of largest mortgage underwriters, reports earnings Friday morning.
Stock indexes were lower for most of the day after claims for unemployment benefits rose unexpectedly for the first time in three weeks. The Labor Department said 412,000 people applied for unemployment benefits last week. Economists expected claims to fall.
Applications for benefits peaked at 659,000 during the recession and have dropped by roughly 6% in the past four months.
Ford Motor Co. (F) fell. The automaker announced that it was expanding its recall of its F-150 pickup truck because of a problem with air bags. Fords F-Series truck is the best-selling vehicle in the U.S.
Among companies reporting earnings, toy maker Hasbro Inc. (HAS) fellafter reporting that its first-quarter profits slumped 71%. The results fell short of analysts estimates.
Grocery chain Supervalu (SVU) said quarterly profits and sales fell compared with a year ago. Analysts expected earnings to be worse. Supervalu rose 13%.
Google Inc. will report earnings after the market closes.
Data-storage company Iron Mountain (IRM) rose. Iron Mountain replaced its CEO after the hedge fund Elliott Management called for a strategic review of the business.
Rental car company Zipcar Inc. shot up nearly 56% on its first day of trading.
Google Inc. fell 4%in after-market trading after the Internet search company reported earnings that missed estimates.
Copyright 2011 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.
Posted | Updated


text:
featuring: BANKS, Braids, Reptile Youth, Akiine, Asante Phenix, Trails and Ways, Here We Go Magic, KLP, Sia, Oliver Nelson and Bloc Party
Tag Archives: Here We Go Magic
featuring: The Japanese House, Leo Kalyan, Aquilo, World Champion, Superhumanoids, YACHT, !!! (Chk, Chk, Chk), Disclosure, Cosmo's Midnight and Jamie XX
featuring: This Is Head, Unknown Mortal Orchestra, Kate Boy, FOALS, Tkay Maidza, Janet Jackson, Miami Horror and Disclosure
The Hives
Olympic Ayres
Elizabeth Rose
Sam Sparro
The Rapture
Kris Menace
Simian Mobile Disco
After months of speculation, the line-up for Splendour in the Grass 2012 has arrived!
A Different Ship is the sophomore album from Brooklyn indie crew Here We Go Magic and its release is drawing ever so close. The band managed to score Radiohead producer Nigel Godrich for the record and have dropped track 'How Do I Know' for your audible consumption: _ 'Make Up Your Mind' was the first single unleashed: […]
Indie-rock Brooklynites Here We Go Magic are are putting down the finishing touches to third studio album A Different Ship.
Indie five-piece Here We Go Magic have a new EP dropping the end of March – The January EP. The five tracks were recorded live to analog tape in a New York living room at the same time they were producing their 2010 critically-acclaimed sophomore LP, Pigeons. Here's 'Hands In The Sky': And the music video for 'Casual' taken […]


text:
Catering $70.00
G & M Custom Meats
Reno, Nv
Beef Slaughter $125.00
Pig Slaughter $70.00
Sow & Boar $90.00
Cut & Wrap .70lb
All meat double wrapped.
Lamb & Goats $100.00 kill, cut & wrap
Smoked Meats $1.00 lb (finished weight)
NO TRAVEL FEES to these areas!
Reno, Sparks, Cold Springs, Red Rock, Lemmon Valley,
Panther Valley, Spanish Springs, Washoe Valley, Pleasant Valley, Steamboat.
Clean on-site slaughtering, everything hauled off.
Can also have animal hauled off to our site to slaughter if needed.
Travel fees may apply!
We have whole or half pigs for sale.


text:
When you're pregnant, you're most likely to worry about some big things. Is your baby healthy? How bad will labor actually be? You're also likely to fret about some little things. Will your stretch marks go away? What's up with the hair on your tummy? You might even contained that you worry about things that seem too awkward to go over at all. Why do you burp so much? Why do you pass gas at bothersome times?
First of all we would like to mention that smelly gas often comes on vegan diet, after eating, at early pregnancy, with bloating, for days, with stomach pain, with constipation, at night, with soft stool, after eating dairy, after drinking milk, after eating eggs, after probiotics, after pregnancy, and ibs, and bad breath, and burping, bloating, blood in stool, but can't poop, etc. Let me assure you that you're not the only one who has problem with smelly gas in pregnancy.
Some pregnant women could go into a burp contest with a group of 10-year-old boys and win! Take me, for example. When I was pregnant with my first baby, I attended on a radio talk program. I burped throughout the entire show, leaning back from the microphone and hoping the noise had not been on the air. And it isn't really simply burps that come at bothersome times. Flatulence can be unanticipated and embarrassing, too.
What are in the article?
Causes of awful smelly gas during pregnancy
In early pregnancy, before your uterus is big enough to crowd your intestinal tracts, pregnancy hormonal agents are difficult at work. The boost in progesterone slows digestion, offering more time for gas to be produced. Your body responds by removing the gas with burps and farts. This gas can also result in a sensation of bloating, especially after a large meal.
As your uterus enlarges, your intestinal tracts are shifted and crowded in your abdominal area. This slows digestion a lot more. Your broadening uterus likewise presses on your stomach, which can increase a sensation of bloating.
As if this weren't enough, the muscle-relaxing effect of pregnancy hormones leaves you less able to control the passing of gas with bad odor. Prior to pregnancy you may have had the ability to hold your gas, now your muscles don't react as well. This can result in some humiliating moments.
How to relieve or reduce gas during pregnancy?
It's not helpless, though. Aside from blaming it on the dog or your partner, you can lower the results of smelly gas in pregnancy during first, second or even third trimester:
- Get moving. With your healthcare carrier's OK, workout can promote food digestion– helping things to move along faster.
- View what you eat. Prevent foods that have the tendency to cause gas, such as fried or fatty foods, onions, cauliflower and cabbage.
- Watch what you consume. If milk appears to cause gas, attempt drinking it icy cold. Keep soft drinks to a minimum.
- Do not eat too much at once. Attempt smaller, more regular meals.
If gas in pregnancy feels more like abdominal pain at any point or you discover severe diarrhea or blood in your stool, consult your healthcare company.
What other says about horrible gas during pregnancy
Story #1: I am usually able to discreetly anf quietly pass gas, and it hardly ever smells. For the last month approximately, my gas has been frequent, loud, and smelly whenever. I suggest it stinks like rotten eggs, that sulfuric smell. I cant think about anything I have actually been consuming more of lately that would cause it. I am pooping every other day (which is quite regular for me). I can barely stand the smell, and it gets truly embarrassing in public too. Am I the only one laying rotten eggs?
Story #2: The past few days I have been extremely gassy and smelly. Have any of you experienced this or is it simply me?? If you have, were you able to do anything making it stop?!


text: I would like to suggest a facility for moderators to close (or edit-lock) individual posts so that the author can no longer make any changes to it. For example, when you remove part of a post and the member edits it back in again afterwards; such as removing a NSFW image that the member feels should be allowed. Cheers, Shaun


text:
It only takes a minute to get started. Fill out our simple sign up form and login to the site.
This step is discretionary but will seriously boost your chances of your profile getting read by other members. Introduce yourself, your hobbies and upload your best photograph.
Utilise our website's state-of-the-art search to locate members who meet your requirements. Or just look through listings and locate folks that you find attractive.
Located someone who you're attracted to? Send an email to them or just a wink and see if anything happens. With a little luck you'll be on your way to a lifetime of happiness.
It's really quick


text:
Mr Luiz Antonio Nali
Brazil, São Paulo - Brazil
No approved endorsements
About Mr Luiz Antonio Nali
•Master's Degree in Administration and Planning – PUC/SP
•Post-Graduate Degree in Financial Administration– FGV/CEAG
•Post-Graduate Degree in Business Administration - USP
•Bachelor's Degree in Accounting Sciences (CRC 1 SP 193496/0-6) – FIG
-Vice-President, CFO and Administrative-Financial Executive in Leasing Company.
-Career in large-sized multinational companies: -Warner-Lambert, Wyeth-Whitehall, Grupo ABN AMRO Bank and Computer Sales International.
-Managed the areas of Accounting, Costs, Budget, Tax, Financial Planning, Treasury, Credit & Collection, Insurance, IT, and Human Resources.
-USGAAP balance sheet/result projections.
-Executive Director approved by the Central Bank to manage leasing companies.
-International experience with trips to USA, Holland, Portugal, Tunis, and Spain.
If you notice offensive or incorrect information, please contact us.


text:
Evan Wilds. The name fit the personality. He was a complete and utter asshole. Girls were sex objects, something he only had for one night and then it was over. He basically ruled the school. The girls drooled after him and the guys wanted to be his friend. Except some of the douche jocks but he could care less. Life was good for him. He came from a family of money so that was never an issue, he also came from good looks too and sex was as easy for him to get as it was to snap his fingers. He liked life the way it was. He was also Alpha to the biggest werewolf pack in the country. But that little detail he saved from the humans that attended the college that was supposed to be made for werewolves but somehow they weaseled their way in. Cora Stevens was the complete opposite. She lived with her grandma in a small house and had a waitressing job at a small coffee shop close by her house. She had gotten a scholarship to a very good college and her grandma had talked to the lawyers about getting her inheritance money from her parents early so she could get a dependable car that would get her to and from school and work. She was a good girl and had never had a boyfriend in her life. She knew she wasn't like the other girls, and she was quieter and not as adventurous. She just wanted to graduate and live her life. She didn't realize the bad boy of the school would soon have his eyes set on her and only her and disturb her peaceful life completely. But is it for the better? Or worst?
WiseSeal Jul 07
Carter's better, he's smarter and so cute!!! I love him!!! 😍
ThatDamHunter Jun 07
Literally everyone loves Carter from just a few lines, me included
Familyfun24 May 07, 2016
Honestly, can I please just have Carter? That is the cutest thing ever!
rifak13 Apr 27, 2016
Love guys with a little temper (not psychotic murder temper)
bubbles339 Jun 24, 2016
Wanting to get laid, I think that has already been accomplished many, many times
GodlessHearts May 08, 2016
Why can't Evan be like his brother? At least he got some sense.


text:
super flat top sunglasses age across the whole of the eye area, nice brown lens so you definitely will not squint wearing these. All glare removed. Great value for the money. Pleased with this purchase. I bought these as I needed a stronger pair of sunglasses as I can be heavy handed with them and they break, these looked more sturdy, I was pleased when they arrived and they look good. My only grumble is that they are quite heavy as far as sunglasses go but I suppos Mexico what is the meaning of this in hindi ralph lauren t shirt he glasses excellent buy, wish the had a brown case would have bought the brwon glasses and the brown case also. It is great case and beautiful color fit my large sunglasses in them . the hard case is brilliant as it is impossible where ever you pSingapore city discount designer glasses kite surfing equipment .
and they come in a plastic case, with a cleaning cloth. Quality good, price ridiculous, and convenience great, hence five stars.
Very good for the price, not to dark but good anti-glare .Fits on most glasses with no proble ralph lauren watches n you realise why it is just 3 pounds! the glass is like been tainted by spray paint lol but any way i bought it for the look not the quality of the glass. another point to add, it took almost a month for it to be delivered. would give 5 star if it was delivered bait earlier.
I've had similar glasses a while ago, but I had misplaced them and wanted to get a new lady gaga sunglasses he combined pair will be black as no light should be able to get through the combined lenses at that specific position. Nothing like this happened as far as I could tell, so I can only assume the lenses of this product are unpolarised.
I've been very short-sighted since childhood, and have paid through the nose for prescription wrap-around sunglasses which become useless as my vision changes. I now have two pairs of these clip-ons, which do a marvelous job of reducing glare .


text:
I have one location that seems to always have problems with dropped Citrix sessions and other connectivity related troubles. Their router and the provider's equipment aren't detecting faults in the internet circuits and their bandwidth usage is low, so we're looking at the building's wiring as a possible source of issues.
There are free ping tools that show packet drops, but is there a (hopefully free) tool that can do more complex traffic analysis and show things like checksum failures on packets, re-transmits, etc? The more information we can collect, the better, and I don't think that just pinging will be enough to fully nail this down. Any recommendations?
Dec 22, 2011 at 1:49 UTC
Internal I.T. Ltd is an IT service provider.
check out wire shark http:/
6 Replies
Dec 22, 2011 at 1:49 UTC
Internal I.T. Ltd is an IT service provider.
check out wire shark http:/
Dec 22, 2011 at 2:30 UTC
check out http:/
Dec 23, 2011 at 10:19 UTC
+1 for Wireshark. A bit of a learning curve but an excellent tool.
Dec 23, 2011 at 12:01 UTC
1st Post
I would have to third the suggestion of wireshark.
Dec 26, 2011 at 6:52 UTC
Wireshark is a great tool, but it's a low-level tool. Fundamentally, it's a packet sniffer, so you need to figure out what you're looking for. Check out Youtube for videos of people using it to solve specific problems to get a handle on how it would work.
Dec 27, 2011 at 7:53 UTC
Thanks all. I'll work with Wireshark once I have a better idea of what I'm looking for, but I believe that to start with, a couple of the Colasoft tools look promising. Start at a high level and dig down to details using Wireshark once we isolate whatever's causing the problems.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.