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IBM Stock Presents A Great Opportunity For Income Investors
- Its not all doom and gloom over at IBM. Q3 earnings showed that its GTS grew revenues to almost $8 billion. Profit margins also rose.
- Once international markets pick up again coupled with dollar weakness, IBM could very easily return to revenue growth without doing much on its part
- Use the recent SEC announcement to scale into this stock. IBM is down $10 a share since October 20th
- Income investors should avail of the attractive 3.7% yield on offer. Furthermore, I believe the stock is cheap and has the cash flows to keep rewarding shareholders even if the stock remains at these levels.
IBM (NYSE:IBM) announced earnings on the 19th of October and although most analysts continue their bearish stance on the company (due to declining revenues and net income), I see positive signs ahead of the company, especially from the perspective of income investors. IBM stock is now down almost 13% year to date (see chart) and may drop more before years end as recent guidance for FY2015 is indicating that revenue will continue to be under pressure in the near term.
Source: IBM stock price data by amigobulls.com
However, this company is making changes internally (that many analysts are ignoring) and I'm confident these changes will bear fruit in the years to come. As a result of these changes, gross margins improved by 80 basis points to 50% in the third quarter of this year. Let's go through some recent developments at the company whilst also understanding why IBM stock, currently at $140 a share, offers a great opportunity for income investors.
Firstly, the technology services division (GTS) grew revenues by 1% (YoY) up to $7.9 billion. The return to growth was due to improved performance in security and cloud which came as a result of larger deals having been signed over the last 6 months. Furthermore, double digit growth was seen in its soft-layer business (outsourcing & integrated technology) which again looks very promising. The shift going on here is clients becoming more interested in outsourcing infrastructure and IBM can take advantage of this trend with its embedded cloud and mobile initiatives.
I think its important to point out the GTS revenue growth because all this stock has had over the last 3 years has been negative analysis because of declining revenues. I acknowledge that the stock is where it is because guidance and profits are also declining but I just feel at $140 a share, this stock is undervalued. Why? Well, firstly, even though reported revenues fell by 14% in Q3 of this year (year over year) but when you adjust the earnings for the strong dollar, the decline was a mere 1%. Also this company has been busy in divesting businesses it doesn't want any more ($7 billion of divestitures in 2014 alone) and even though I believe these business and asset sales were the right decision (because of low profits or being unprofitable), they obviously affected top line decline which we are seeing this year.
Then you have international markets like the Bric countries (Almost an 11% drop in revenues from Brazil, Russia, India & China in 2014) that have declined meaningfully in the past few years. Again the downturn internationally is something that IBM can't control and when you combine the international downturn with weaker international currencies, it was evident that the company's top line was always going to suffer especially when you consider the huge percentage of international sales this company does annually. Nevertheless, what investors shouldn't overlook is that if the bric's can turn up and the dollar weakens from here, IBM's revenues could easily grow again without the company doing much on its part. The divestitures will ultimately slow down, which should help in printing a bottom for revenue declines so the company can once again return to sales growth.
Then you have the recent SEC investigation which definitely has affected the stock adversely over the last week or so. It was ironic that on the very same day the SEC investigation was leaked, IBM authorized a $4 billion increase to its share repurchase program which now brings the total authorization outstanding to $6.4 billion. The stock has remained weak though and between earnings and now with the SEC debacle, the stock is down $10+ a share since the 20th of October. Use this downturn in the stock to go long.
So at $140 a share, do you believe this stock offers substantial upside or do you believe it is a dinosaur like so many bears would have you believe? Well, I am the former and here is why. The stock looks significantly undervalued at its current P/E ratio of 9.63. Long term investors need to look at the fundamentals over a 10 year period (see table below) and not just over the last 14 quarters where the company has had declining revenues. Let's see if the growth trends stack up for all the important fundamental metrics over a 10 year period.
|Years Of Dividend Increases
|19 Years - Pass
|Free Cash Flow
|$3.4 billion (Expected Dec 2015) (10-Year Trend Is Up) - Pass (Very Important For Dividend Investors - Dividend Currently Is 3.7%)
|$86.9 billion (10-Year Trend Is Up) - Pass
|Profit Margins
|13% - (10-Year Trend Is Up) - Pass
|Price History of the stock
|Up 72% in the last 10 years excluding dividends - Pass
|Healthy balance sheet
|Total assets = $112 billion (10-Year Trend Is Up) - Pass
|Competitive Advantage
|Resistant to recessions?
|Net Income rose (see chart below) during the recession of 2008 - Pass
Source : Macroaxis.com
Therefore the table above shows that none of the important fundamental metric have declined over the past 10 years which begs the question - why do so many analysts believe this stock is doomed just because it has had 14 quarters of declining revenues? Astute investors understand that the company is currently getting smaller (similar to Procter & Gamble (P&G) (NYSE:PG)) so it can grow quicker in the future. We can see this in its gross margin metric (currently 50%) which has risen in the past few years. This is why free cash flow levels have remained flat over the troublesome period and this is the one true metric really that income investors should be interested in.
The company has managed to achieve a dividend growth rate of almost 14% over the past 3 years which illustrates its commitment to shareholders even in the face of declining revenues. Secondly, its price to earnings and price to sales ratios have never been lower meaning you are getting the stock at a very attractive price. Do not listen to the bears that state that IBM's stock is elevated solely because of the "financial engineering" its accountants undergo. I refute this. This company has a history of rewarding shareholders. This is probably why its entry into the cloud space was slow (compared to competitors) because it put its shareholders ahead of potential revenue growth. Thirdly, its free cash flow levels are expected to be higher this year compared to 2014 even though revenues will be lesser. This is why the dividend pay-out ratio has remained small over the past 3 years. This company is a cash cow and the growing dividend in my opinion is ultra safe.
To sum up, I believe IBM stock presents an excellent opportunity for income investors here. Its free cash flow levels and high dividend growth rate will ensure shareholders get rewarded handsomely over time. Furthermore, its fundamentals are still very strong so its present 3.7% yield looks very attractive at these levels.


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Product Description
Specification :
Specialty of Love Moon Anion /WinIon Sanitary Pads
1. Comfort Level Of Anion Sanitary Napkin
Love Moon is made of soft cotton that is gentler, thinner and much comfier. Majority of the sanitary napkins in the market is made by the chemical fibre surface or rough cotton surface. You will feel more comfortable with Anion Sanitary Napkin.
2. Air Permeability Of Love Moon/WinIon Sanitary Napkin
The base layer of sanitary pad is permeable to air but not to water. This air permeable layer is to prevent for bacteria multiplication and gynaecological diseases.
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The cotton surface of Love Moon / WinIon Sanitary Napkins can absorb twice as much fluid as normal sanitary pad. The super absorbent polymer can hold the penetrated water in, instead of let them side leak or back flow.
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Anion strip in our product can emit high density of anion when in use. The released oxygen can suppress the major source that causes gynaecological diseases.
5. Anti-Counterfeiting Of Love Moon Sanitary Napkin
To protect our customer from low quality counterfeits, Anion Sanitary Napkin is featured add-in bio-magnetism substance in the anion stripe which has an anti-counterfeiting function.
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• You can return your purchase for up to 7 days from the purchase date. If you purchase on 1st Jan then you have until 7th Jan to return those orders.
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subset: commoncrawl


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Greeting to all and welcome new friends to the EastWing.
As the birds flock together and the waning days of August are winding down, so too a season is winding up. Now what's most interesting about that first sentence is both winding up and winding down point in the same direction. With up and down, words that normally mean complete different directions, going in the same direction, it's no wonder we had a president who most famously said "It depends on what is is". That's an example of why many think American English is one of the most difficult languages to learn to speak and even more difficult to understand.
Last Friday evening the plan was to watch the Packers play football at San Francisco. The normal pregame stuff was said and done then when it's time to play the National Anthem, Colin Kaepernick, the 49ers quarterback refused to stand. Said he believes the United States oppresses African Americans and other minorities. Make no mistake about it, his actions were just a very small part of his freedom of choice as enjoyed by every American citizen. His actions reflected his opinion of the nation he lives in. Such a poor choice on his part. Now every time the National Anthem is played in his presence, all eyes will be upon him.
"I am not going to stand up to show pride in a flag for a country that oppresses black people and people of color," Kaepernick said. "To me, this is bigger than football and it would be selfish on my part to look the other way. There are bodies in the street and people getting paid leave and getting away with murder." That was his verbatim quote for his actions.
What was not said on TV at this time was this person received a contract to play football and he's being paid $114,000,000.00 Yep that's 114 million over 6 years. With a minimum guarantee of 61 million no matter what. It's truly amazing that society will pay such sums to people who possess such limited skills outside playing football. Colin Kaepernick may or may not be able to converse at a level above 5th grade. I tend to think he would be comfortable at that level of intellect. His actions in this matter supports my inference.
Chances are great that, except for an unusual ability to play football, this man could well be in a much worse state in life, or in jail. Can't help but wonder if he has ever one time in his life has given thanks for what he has received in life. I doubt it.
"There are bodies in the street and people getting paid leave and getting away with murder." That's such a stupid announcement to make in response to not standing for the National Anthem. The only place where bodies are in the street is in the urban black community. Those bodies put there by urban black men. Like the facts or not, black men make up less than 10% of the total population and they committee over 50% of all the murders in the nation. That's FBI fact sheets, not from the EastWing.
Another twist to this sad story that the NFL and political correct news media won't touch for love nor money is the fact that last off season, Colin Kaepernick converted to Islam and is currently engaged to an activist in the higher echelon of the Black Lives Matter group. Both Islam and Black Lives Matter are both opposed to any recognition of respect for the United States.
The only reason we can have a worthless piece of crap like Colin Kaepernick disrespect not only the National Anthem, but also the whole country is because of those who have fought and paid the price with their blood for today's freedom. Even when that freedom is flaunted in the face of the National Anthem, on national TV. Even when the offender may well be to dumb to realize what he's doing.
Yesterday marked the 240th anniversary of one of the most significant events in American History. An event where the very survival of this nation tittered on the outcome. Yet it's importance has long since slipped from our collective memories. So now most don't even know it ever occurred. I tell this story here to illustrate the degree of human sacrifice that was made for generations yet unborn, by the men who fought for the freedom of setting down while the National Anthem is being played at a football game 240 years in the future. The first major battle of the Revolutionary War it was.
On August 27, 1776, the Battle of Long Island was fought. The British force was in excess of 34,000 troops. George Washington's Army numbered near 12,000. It was not a good outcome for the Patriots.
Marching through the night, the British took the Americans by surprise and overwhelmed most of their lines. George Washington, watching the enemy cut down his men, cried out, "Good God, what brave fellows I must this day lose!" That day 2,400 Americans died on the battle field.
Only herculean resistance by some 400 or so Maryland and Delaware troops avoided a total defeat. "If a good bleeding can bring those Bible-faced Yankees to their senses, the fever of independency should soon abate," one British officer predicted.
The Americans retreated to Brooklyn Heights, where they sat on the verge of total disaster. It was here that General Washington realized that he must somehow get his army off Long Island or lose not only the battle but also the war.
British warships were prepared to sail up the East River, which lay between Long Island and Manhattan, to cut off any retreat. Fortunately for the Patriots, strong winds kept the ships out of the river, but Washington realized that it was only a matter of time before he was trapped.
The general ordered a search for all available boats. On the night of August 29, under cover of rain, oarsmen began ferrying the army across the East River. One Connecticut officer remembered making eleven trips across the river that night, carrying men to safety. The troops hurried along in complete silence. They knew full well that if discovered in retreat, the Patriot force would be annihilated. The last American to leave the Long Island battle field was George Washington.
Only a fraction of the army had crossed by daybreak. As historian David McCullough wrote, "Incredibly, yet again, circumstances—fate, luck, Providence, the hand of God, as would be said so often, intervened."
A heavy fog settled over Brooklyn, obscuring the American movement. When it lifted, the stunned British realized that over 9,000 men had slipped into the fog and out of their grasp. The bruised Patriot army would live to fight another day. A new nation had survived it's first battle for freedom.
I venture to say Colin Kaepernick never heard that story or any other story of why he can sit on his rich ass and make disparaging remarks about the National Anthem and the country who has provided him with so much in his short life. Shame on him.
From The EastWing, Winding Up & Winding Down, Bad Manners, Freedoms Choice, An Old Time War Story
I Wish You Well,


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- Duration
2 - 3.5 hours
- Group Size
Minimum of 10 hens.
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7 days a week
- Seasonal
All year round
- Dress Code
Bring your vintage clothes
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Studio or your self-catering apartment
Please note...
Please Note: This page provides a general guide to our vintage makeover in Brighton. We work with multiple suppliers, so please be aware that specifics may vary. Feel free to ask your Destination Specialist at the time of booking if you have any questions about this.


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From their 1700's London origins to their 1800's Burton heyday through their 2000's Californian rebirth, IPA has remained one of the world's most evocative, exciting and encapsulating beer styles. IPA is our go-to style here at BrewDog; from Punk to Hardcore, Jack Hammer to Atlantic IPA, we have majored in bringing you as many hop-forward beers to you as is humanly possible..
We wanted to brew a completely new and conceptual IPA that paid tribute to the style's origins whilst incorporating the devastatingly modern techniques and technologies. A 21st Century drink with an 18th Century ethos; a very new yet very old IPA.
- Delivery
Delivery is available throughout the United Kingdom with costs as follows (all including VAT).
|Collect from Shop (Glasgow)
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Local deliveries are available in the Glasgow area for orders over £20. Deliveries are every Wednesday from 6pm to 9pm. Orders must be received by 10pm on Tuesday. To check if your postcode qualifies please refer to the Local Deliveries spreadsheet.
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Here are 2 fun new titles for kid's – host a Kid's Movie Night with some popcorn, blankets and family fun!
'The Bears are back' in an all-new CGI animated series Care Bears: Totally Sweet Adventures debuting on DVD, Digital Download and Video On Demand March 5 from Lionsgate Home Entertainment. The Care Bears embark on all-new exciting adventures with a fresh new look, new attitude and all-new music. These lovable Bears premiered on The Hub TV Network in June 2012 and garnered over 6 million unique views. Re-created with CGI animation, this all-new DVD includes a share and care sing-along in addition to the four adventures. Come back to Care-a-lot and join Cheer Bear, Share Bear, Tenderheart Bear and all of the Care Bears in Care Bears: Totally Sweet Adventuresavailable on DVD for the suggested retail price of $14.98.
I was a big Care Bears fan in my youth and thought this DVD was super cute and fun! Once I told Noah how his Uncle Phil had a Care Bear and they weren't all for girls he was in for watching and I think he enjoyed it!
Experience a samurai showdown as the Red Rangers go head to head in this special movie event, Power Rangers: Clash of the Red Rangers – The Movie arriving for the first time on DVD and Digital Download March 5 from Lionsgate Home Entertainment. In 2013, Power Rangers will celebrate 20 years of global success as the #1 kids live action series. When a mysterious RPM Red Ranger appears, the ultimate battle to fight off the threat of Master Xandred's Mooger army begins. Action packed and loaded with high-energy entertainment, Power Rangers: Clash of the Red Rangers – The Movie will be available for the suggested retail price of $14.98.
This movie was right up Noah's alley and he sat captivated through the whole thing. It is a very high energy movie and has asked to watch it again!
Enter the Giveaway!
You can win a copy of the Power Rangers and Care Bears DVDs! Please enter the giveaway on the widget below. Email and feed subscribers will need to click over to my actual site to enter.
I received the same prize as the winner. All opinions remain my own.
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Hump Day Mixed Case
Your Hump Day Mixed Case includes one bottle each of
Longchase Vintners Cabernet Sauvignon
This 2013 Vintage Cabernet is a light red with flavors of cherry, raspberry and cinnamon, and a medium finish. Enjoy this versatile wine on any occasion, whether you're ordering in from your neighborhood pizza joint or grilling with lots of friends.
Anta da Serra Red Blend
Anta Da Serra is for wine lovers seeking a bold red with flavors of blackberry, raspberry, and cherry. This soft, full red blend has a deep color, and retains the characteristics of the Portuguese grapes that make up its lush character.
Kepe Tempranillo
Think of this ripe, Spanish-style red as the guest who takes the festivities to the next level. Raspberry, strawberry, plum, and black currant flavors start in the aroma and get everyone dancing through to the party's satisfying finish. Open Kepe Tempranillo anytime and invite it by to enjoy your favorite foods like pizza, pasta, and grilled meats.
Cachil Cabernet Sauvignon Reserva
Chile's Colchagua Valley is known for gorgeous Cabernet Sauvignon, like this reserve offering from Cachil. Cachil Reserva Cabernet Sauvignon boasts beautiful flavors of currants, raspberry and cassis with hints of cocoa. It's enjoyably lighter than the average cabernet, with a medium body and smooth finish. Taste this excellent red and you'll understand why Colchagua Valley was named Wine Region of the Year by Wine Enthusiast Magazine.
Geja's Private Reserve Big Bold Red
As the name says, this feisty wine is big on bold, ripe flavor. Geja's Big Bold Red is fresh and forward, with prominent, aggressive tannins and balanced acidity. If you haven't already tried it, there's no better time than now. This is one wine you can enjoy every day of the week.
Rivers End Reserve Merlot
This wonderful red is the versatile bottle you want on your shelf to go with almost anything you cook, especially grilled fowl, braised red meats, and rich tomato sauces. Note the ripe berry flavors and aromas, and the nice touch of oak.
MYTH Riesling
The intense sun and cool pacific winds of Washington state's Cascade mountain range optimize ripeness and sugar levels in nearby vineyards, resulting in fruity, rich wines. Myth's newest Riesling entices your nose with aromas of nectarine, apricot, and honey leading into flavors of honey crisp apple and stone fruit. Riesling is an inherently versatile white, begging to be enjoyed at a cocktail party or with Thai and Indian-inspired cuisine.
Bear Hug Winemaker Select Chardonnay
Chardonnay is the world's most versatile white grape, with variations depending on the growing region and picking time. Within each lot, a coveted grouping is bottled separately and known as Winemaker Select. This is often the most accessible and enjoyable Chardonnay that you can get. Bear Hug Winemaker Select California Chardonnay is our best of the best. From the first sip, you will find flavors of green apple, nectarine, honeysuckle, and melon, all in a perfect harmonious balance.
Blume Sauvignon Blanc
From the plains of the Iberian Peninsula comes Blume Sauvignon Blanc, a fresh Spanish white with juicy peaches, mangoes, herbs, and hay on the bouquet. The lively fruit flavor and crisp finish make it the perfect companion for goat cheese, grilled veggies, or anything from the ocean.
La Petite Tour White
La Petite Tour winery has been a highlight of Calfornia's wine scene since the 1980s. Over the years, this white blend has remained a family favorite, and was originally only made in small lots for their own table. La Petite Tour Classic White is a delicately aromatic, medium-bodied wine with flavors of ripe pear and surprisingly bright citrus notes. It's a versatile food wine, that pairs well with fish, grilled chicken or vegetables, and we've found that it's the bottle that thrills white wine fans…and converts red wine drinkers.
Della Flora Toscana Bianco
This vibrant and refreshing Italian white blend features 70% of Trebbiano Bianco, the aromatic grape that produces top-tier Cognac and Armagnac. With its pale, straw hue and enticing aromas, Della Flora Toscana Bianco gifts the drinker with flavors of melon, green apples, and lemon zest, finishing crisp and refreshing. Pair it with fresh seafood.
Big Cirrus Chardonnay
Chilean Chardonnays are picked earlier, capturing a green essence that sets them apart from their California counterparts. Big Cirrus flaunts vibrant green apple and citrus zest flavors with a clean and smooth finish that lingers on the palate. If you prefer crisper Chardonnays, Big Cirrus Single Estate is your next white wine.


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econ industries offers solutions for the treatment of industrial wastes and contaminated soil. Our VacuDry technology separates harmful substances from the solid material with heat and vacuum in a fully encapsulated system. Regarding energy efficiency and carbon emissions, this method is far superior to other thermal desorption techniques and is accepted as state of the ...
based in Starnberg, GERMANY.
Tetronics International is an environmental company with 50 years' global experience delivering clean plasma technology for maximum resource recoveryand the highest levels of hazardous material destruction. This brochure focuses on Tetronics' solutions for managing Air Pollution Control residue (APCr)/Incinerator Fly Ash. Tetronics has a proven, turnkey capability, from ...
based in Swindon, UNITED KINGDOM.
Hydro Quip, Inc. (HQI) offers a complete line of high efficiency above-ground and below-ground oil/water separators in a complete range of sizes and options, constructed of carbon steel, stainless steel or fiberglass. The separators will remove essentially all free dispersed non-emulsified oil and settleable solids from the waste water and produce an effluent to meet the ...
based in Seekonk, MASSACHUSETTS (USA).
IMABE IBERICA S.A. is a leading European company in the manufacture of a complete range of equipment for the treatment and recycling of solid waste. With over 40 years of experience in the market, Imabe Ibérica has developed a technology for exceptional quality and reliability to reduce the volume of waste nowadays offering a wide range of machinery. Imabe Ibérica is one ...
based in Arganda del Rey, SPAIN.
Europlasma designs and manufactures plasma heating systems which high T° can have an application in steel making industry, waste destruction, high T° gasification. The Waste To Energy Europlasma solution Based on 15 years experience in industrial waste plasma processing and the know how transferred from EADS, Europlasma has developed a robust and efficient waste plasma ...
based in Morcenx, FRANCE.
The Bukit Uldal Material Recovery in Tutor, Brunei Darussalam is a specialist environmental services in hazardous waste management to oil & gas industry in Brunei Darussalam. We provide disposal treatment for drilling and production waste such as oil based mud, chemicals, contaminated soils from spill response.
based in Tutong-Telisai Highway, BRUNEI DARUSSALAM.
Norsas AS is the Norwegian Resource Centre for Waste Management and Recycling. Norsas is owned by the InterConsult Group AS. The company is located in Oslo.The company was established in 1988 with the principal aim of setting up a national network for the reception, collection and treatment of hazardous waste. Since 1994 the company has developed into a national resource ...
based in Etterstad, NORWAY.
Alaknanda Technologies is an organization that deals with providing low cost solution for various challenges of the society. We provide strategic solutions in private sector, government and others through our team of diversified knowledge and experience. We have a diversified team consisting of Engineers, planners, environmentalists, biologists, botanist, software ...
based in Thane, INDIA.
KAB TAKUMA GmbH is a power plant construction company with decades of experience, the roots of which go back to the year 1951. KAB TAKUMA directs all its efforts towards one overriding goal: the development and implementation of advanced solutions for the generation, conversion and distribution of energy. We are specialised as general contractor for the construction of ...
based in Berlin, GERMANY.
Providing services to oil, gas, power generation and Maritime industries in the middle east and north African region; in soil remediation,sludge,tank and tanker cleaning,waste water treatment,and hazardous waste management.
based in Dubai, UNITED ARAB EMIRATES.
Activities performedComplete waste management-services (sanitation, collection, transport and final disposal of hazardous and non hazardous waste);Cleaning and reservoirs repair works;Civil, industrial and agricultural construction;Construction-assembly works;Desilting and earth works;ADR-specialized transport;Service of drilling fluids treatment and preparation.
based in Marghita, ROMANIA.
We are a leading contractors , suppliers , importers our main fields of business are: water and waste water treatment plants construction (turn key) pumping stations for water and waste water hazardous waste remediation , hazardous waste transportation heavy metal remediation , environmental engineering.
based in Mansoura, EGYPT.
WasteServ Malta Ltd. was established in November 2002. The company is responsible for organizing, managing and operating integrated systems for waste management including integrated systems for minimisation, collection, transport, sorting, reuse, utilistation, recycling, treatment and disposal of solid and hazardous waste.
based in Santa Venera, MALTA.
Expert service is offered in the following areas: - Transportation, Treatment and Disposal of Hazardous and Non-Hazardous Waste.- Reclamation of Waste Oil and Oily Water.- Resource Recovery through Solid and Liquid Fuels blending.- Waste Management:Sampling,Identification,Labeling,Staging/Repackaging,Analysis.- Lab Packs: Identification,Packaging,Transport,Disposal.- ...
based in West Paterson, NEW JERSEY (USA).
Modern Environmental Services Company is a registered Jordanian Company with its head office in Amman, Jordan, and representative offices in Baghdad, Algiers, Saudi Arabia, Qatar, Greece and Cyprus.MESC is a diversified environmental services company that provides the following services to industry and governments in the Middle East, East & North Africa, the Gulf States ...
based in Al-Abdali, JORDAN.
Ecological Services provides immediate professional services and solutions for treating and recycling industrial waste. Ecological Services operates for the protection and preservation of the environment and incorporates all the means at its disposal in order to optimize industrial waste treatment, maximize recycling, minimize volumes and reduce costs. The transition ...
based in Gedera, ISRAEL.
Creative Waste Management Inc. (CWM) is an environmental service, sludge dewatering company founded by experienced professionals dedicated to providing environmentally sound solutions to sediment dewatering and sludge management problems. Provides a variety of services to industrial, utility, and municipal clients. Developing and applying proven waste management ...
based in Folcroft, PENNSYLVANIA (USA).
OZONE Waste Solutions owns and operates the NG-3000 model OZONATOR in Greensboro, N.C. and represents OZONATOR Industries in the following six (6) States; North Carolina, South Carolina, Georgia, Alabama, Tennessee and Virginia - USA.
based in Greensboro, NORTH CAROLINA (USA).
Staffed with engineers, scientists and technical support staff familiar with regulatory and business/risk management demands, MEC provides engineering and scientific services primarily related to environmental, health and safety issues.
based in Jefferson City, MISSOURI (USA).
BECPL is one of the leading Environmental Engineering & Consultancy company.Company was established in the year 1990. Since then our focus is on sustainable development of Industry and Environment based on sound engineering practices, innovation, quality, R & D and most important is satisfying customers need.
based in Ahmedabad, INDIA.
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text: Shop for Belmont Stakes Betting Odds By SBR Monday, June 6, 2016 Share with: Online sports bettors can shop for the best Belmont Stakes betting odds using Sportsbook Review. The 2016 Belmont Stakes takes place this Saturday, June 11, from the Belmont Park Race Track in Elmont, New York. There is no Triple Crown up for grabs following Exaggerator's upset of Nyquist at the Preakness Stakes. There will also be no rubber match between Nyquist and Exaggerator as Nyquist has been ruled out of the race due to having an elevated white blood cell count. Exaggerator is an average -115 favorite (a $115 bet returns $100) to win the Belmont Stakes. Bookmakers expect a relatively tight contest rounding out the two through four slots as Suddenbreakingnews, Stradivari, and Cherry Wine can all be had for around +750 to +1000 with a bit of line shopping. By using the Belmont Stakes odds directory, bettors can compare horse racing odds from top online sportsbooks.


text:
Seoul Hiking Nature Group
- main resort for the 2018 winter olympics, best&largest in korea,
- deeply snowed,10minutes by bus from resort, snowy nature hill, ice frozen river, crunchy crunchy snow hike up the horse farm and sliding on nature slope! BBQ party! sheep farm
- best ski/snowboard gear PREPARED,rental, clothings,LIFT TICKET all at once
#Cheeseburger, making sandwich, Burritos, homyfood
#BBQdinner - beef,cheese,onion,tomato,lots of veggie mix,bun
#breakfast - cheese,egg,bacon,salsa sauce,bun(tortilla)
- 98,000won early bird fee till 25th,Nov to best resort includes all of (Bus, Pension, Lift, SKI/Board rental, Dinner Party~)
- 45% discount for extra slope time,using spa in resort!
Hi~! everyone~!
Seoul HIking Group will take you to the Yongpyeong resort to enjoy ski! and snow-capped mountain pension on Dec,17~18th! We have been for 9th years in a row and got the best deal!
Cities in winter are cold but just couple of hours driving from city it's snow world of full of fun! December is the best time to kick off the bed and hit the slopes and try out what Yongpyeong Ski Resort has to offer!
One of the benefits with going with the Seoul Hiking Group is our group has exclusive access to the rental shops. This makes it easier (and faster) to get your snowboarding/skiing gear; board, boots, poles, pants, jackets, goggles and gloves etc.
Korea is well known for having great ski resorts in the Gangwondo area. When you book alone, you need to reserve a pension in advanced, wait in long lines to get lift tickets, rent gear and find your own transportation out to the resort. However, with the Seoul Hiking Group, we have all that planned and organized already for you~!
Let's enjoy the winter months by enjoying the outdoors. Let's take advantage of Korea's largest and BEST ski resort with the best deal and organized ski trip of seoul hiking group!
** Hurry to join this awesome skiing/snowboarding event.
Spots are limited, there are only 42 spots available! **
❂ Early Bird discount(₩98,000won) is running from now- Friday Dec, 2nd
₩106,000 includes below everything!
❂ Chartered Bus
❂ Lift ticket: All day Saturday12PM - 430PM (GONDOLA included)
❂ Ski or snowboard rental
❂ Pension +Cheese burger dinner + Burrito (or sandwich) breakfast
❂ Discount for the sauna and etc. in the resort
❂ For beginners: guide at rental shop and free instructions on the slopes
❂ (Optional) Sunday skiing/SB lift and rental fee, hanging out near the snowy pension
❂ Free instructions
❂ Great company!
1. Yongpyeong resort:
-Widely known as the best and largest resort in Korea!
-The resort features 28 slopes
-The longest course is 5.5km! (The longest course at the Phoenix Resort is only 2.4km)
-Waiting time for lifts is less than 5 minutes (resorts near Seoul are overcrowded with never-ending lines)
2. Sunday (optional) ski:
There is an additional fee of 48,000won for the lift and ski/snowboard rental. In order to get this package, you need to inform the Seoul Hiking Group in advanced so we can organize this accordingly (payment is required before Saturday night)
3. Free instructions for beginners! There will be free lessons provided for beginner skiers/ snowboarders. Don't worry if this is your first time!
4. Snow capped hill pension!
-anyone just discover hills,ice frozen rivers and enjoy crunchy crunchy walking! Horse farm, the scenic places reminds of somewhere in Mongolia!
we can make some natural slopes, find a hill and with shovel we can make to enjoy natural riding!.
- another things ,to wonder around village,find small cafe all decorated with christmasy trees,jazzy music, have some cup of tea,coffee!
- enjoy sauna at rural city , it would be so healing!
- 8,000won taxi ride to the best well-known Seonjaryeong snow hiking path! also you can visit sheep farm to feed the sheep and take the picturesque picture of whole town
♣ For beginners: Instructions for snowboarding/skiing
Easy as easy to start to learn how to ride bicycles!
Learning how to ride a board (or to ski) is similar to learning how to ride a bicycle! If you believe that you are going to stand up and ride down that slope, you can do it! One of the most important and crucial things about learning how to ski/snowboard for the first time is, you shouldn't be afraid of falling down. It's all about the learning process. On the plus side, unlike riding a bike, you're falling down on fluffy snow!
Once you get the swing of things, you'll be a professional flying down the hills in no time!
-> http://www.youtube.co...
-> http://www.youtube.co...
-> http://www.youtube.co...
Beginner Skiing Lesson #1-1 - Getting Started and Equipment
-will be prepared by the rental shop
-you've already provided your height and foot size, so there will be gear already arranged for the beginners (they will also do a fitting with you to make sure things fit properly!)
-We will choose a fitting slope for the beginners. This means, you have time to practice all together and watch out for each other.
-The best thing is to try and try! FIGHTING~!
- Instructiors and some other experienced members will be around to instruct, guide and help you for 1-2 hours.
-when you finally feel comfortable to do things on your own, you can go check out other slopes!
♣ SAFETY : J-turn slide for Snowboard, S-line for the Ski. going straight is the main reason to crush to others and the accident.
Ski/SB and soccer is the sports that get the most injury among all the sports.maybe except some more in extreme sports.
❂ prepare snacks (I suggest for you to keep some snacks in pockets when you are on the slope for the long ride of lift)
❂ use a SMALL BACKPACK to bring some clothes to change into later (could also be cushion for when you fall)
❂ buy cheap pants: since there is plenty of time before the actual trip, I encourage you to start looking to buy some warm clothing. This includes; windproof pants(and jacket), fleece/wool sweaters etc. You can find some cheap clothing ON gmarket.co.kr or even some underground subway shops. Once you become more experienced, you may want to invest in some expensive, higher quality items.
♣ How to Join
❂ Join the event and book a spot as soon as possible. There are always spots available unless otherwise stated! If we don't have any spots available, we will change the event title to say "FULL".
❂ This is a popular trip, so spots fill up rather quickly. Join the event and book a spot as soon as possible. There are always spots available unless otherwise stated! If we don't have any spots available, we will change the event title to say "FULL".
❂ Please RSVP to the event and wire the money ASAP. First wired and then confirmed~!
1) Join Seoul Hiking Group then RSVP on the event.
2) Please wire 106,000won to my account
Bank name: Kookmin Bank
Bank account number: 762301-04-223562,
Account name: 김승일(Seoul Hiking))
If you are paying the day of the trip, the price is 115,000won (only if spots are available!)
A. ₩98,000: now - Friday Dec, 2nd
B. ₩103,000: ~ Friday Dec,9th
♣ ♣ for those who traveled with us previously:
you're eligible for a discount.
If you traveled with the Seoul Hiking Group before:
1~2times/ -₩2,000
3~4times/ -₩4k won
5~6T/ -₩6k, 7~8T/ -₩8k, 9 more times ~/ -₩10k
♣♣♣ MUST register a form in order to get the proper gear IN ADVANCE !
-> CLICK below to confirm, RSVP, check shoe sizes and then check the confirmed list!
After you've completed the wire transfer please copy and paste and modify it on the Facebook wall
(If you don't want your information visible on the Facebook wall, please send an email to: firstname.lastname@example.com)
------------Copy and modify--------
1. TITLE: Dec 17th~18th Ski/Snowboard Trip To Yongpyeong,snow-capped pension, Free Instruction
(please add names when you wire money for friends)
3. EXACT TIME, DATE and how much ₩ you wired :
4. choose ONE PLACE to be picked up: Hapjeong,Sinyongsan station, Express Bus Terminal,Dongcheon ),
--------------copy and modify-------------
(check below for map)
BUS STOP(choose one) :
650AM:Hapjeong,710AM:Yongsan stn,725AM:Express bus terminal,745AM: Dongcheon station in Jukjeon
Map of pickup for the firsttimers
rental shop
- check gears, rent clothes
- get lift ticket
Ski resort - get the lift ticket from our guide , free instruction
return gear, those who want to ski on Sundayy pick up lift ticket for Sunday
dinner together, Party Party
Breakfast together
- optional, Sunrise hike to behind pension, Horse track, Nature Slope
bus run to rentalshop, shuttle from rentalshop to resort, waterpark
Seonjaryong famous hiking trail, town,
(1 PM : resort : bus pick up at the parking lot
- 12PM check out and move our bags to the front (or rent a locker)
(130 PM : pension & rental shop : bus pick up at the parking lot)
departs for Seoul and arrive approx. at 5~6PM depending on traffic,Gangbyeon(East seoul express bus terminal) / Yongsan station
♣ Refund Policy
CLICK below TO REQUEST A REFUND ->
In order to get a refund you must include the following: Bank information, trip name, bank name, account number and how much you wired.
* There is a 10,000 won cancellation fee for those that cancel 3 days prior to the trip. You do however, have the option to save the money for credit for future trips (avoid the cancellation fee!)
-For those that cancel 48-24 hours before departure, you are eligible for a 50% refund or you can use credit of (50%+10,000) for future trip
-For those that cancel 24 hours before departure you may use 30,000won as credit towards future trips. OR you can be reimbursed 20,000won
*IF you fail to show up, you will not get a refund
Seoul - Korea (South)
Saturday, December 17 at 7:00 AM


text:
2109 N Meadows Avenue, Manhattan Beach CA, 90266 (Residential)
Sold Price:
3 beds , 1 Full baths
984 sqft
Status: Sold
Last Updated: 7/26/2017 9:09 PM
Description for 2109 N Meadows Avenue Manhattan Beach, CA 90266
Prime MB Heights location. Some mountain and City views looking East and South. Sold "As-Is".
Sold Price:
Sold Date:08/24/2015
Los Angeles
1 Full
984 (approx)
Prime MB Heights location. Some mountain and City views looking East and South. Sold "As-Is".
Listing Information
Public Water Service
Sewer Connected
Room Information
Living Room
Interior Features
No Fireplace
Exterior / Lot Features
2 Garage Spaces, 2 Parking Spaces
No Pool
City, Mountain
Curbs Walk
Financial Considerations
Listing Price History
Manhattan Beach Real Estate
Manhattan Beach is located in California. Manhattan Beach, California 90266 has a population of 35,818.
The median household income in Manhattan Beach, California 90266 is $142,071. The median household income for the surrounding county is $55,870 compared to the national median of $53,482. The median age of people living in Manhattan Beach 90266 is 42.1 years.
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text:
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The ACACIA Honey Dubai and Makai bikini set features a full coverage halter top with bikini bottoms. Match with your mini! Honey Clothing (little girl) Size Chart // ...
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The ACACIA Swimwear Andy top is a fixed triangle top with crochet detailing along the borders and ties in the back with tassels.


text:
Published issues of Campbell Systematic Reviews
Campbell Systematic Reviews (ISSN: 1891-1803) is the peer-reviewed online monograph series of systematic reviews prepared under the editorial control of the Campbell Collaboration. Campbell systematic reviews follow structured guidelines and standards for summarizing the international research evidence on the effects of interventions in crime and justice, education, international development and social welfare.
Issues contain reviews only, not protocols or title registration documents. These may be retrieved from the Campbell online library.
Systematic reviews that have been updated are shown only in the volume corresponding to the date of publication of the updated review.


text:
A few days ago, my husband Peter made this tasty Gluten-free triple quinoa bread,...It is a very special Gluten-free bread that includes 3 forms of quinoa,…ooh yeah! There is quinoa flour, quinoa flakes & the bread is topped with red quinoa,…It is smashing in flavour combined with some cheddar cheese on it or some elderflower jam. This bread would also be great food gift! 🙂 This bread is not vegan because of the eggs & buttermilk in here! 😉 This is a very nourishing bread that is very dense but very tasty too! 🙂
Recipe: For 1 medium-sized bread
* Dry ingredients:
220 gr organic quinoa flour
250 gr quinoa flakes
4 teaspoons coconut sugar
1 teaspoon + 1/2 teaspoon Maldon sea salt, crushed
1 teaspoon + 1/2 teaspoon baking powder
1 teaspoon red uncooked quinoa: to top the bread with!
* Wet ingredients:
4 tablespoons water
350 ml buttermilk
2 eggs, beaten
1. Preheat your oven to 190°C ( 375 F ) for 10 minutes. In a large bowl, add dry ingredients together except the red quinoa. That is meant for topping the bread! This means quinoa flour, quinoa flakes, coconut sugar, sea salt & baking powder. Mix well with a larger spoon. In another bowl, whisk buttermilk, eggs & water through each other. Mix well. Now, gradually, whisk your wet ingredient mix into your dry ingredient mix. It will be a very moist dough!!!
2. I use silicon baking forms, so you don't have to oil the tin beforehand, so pour your moist dough into your silicon baking form & scatter your red uncooked quinoa all over the top of your bread! Place into the center of your hot oven & bake for about 60 to 70 minutes until the bread has been cooked through, is golden brown & sounds hollow when you tap on the bottom. Take out of the oven & let it sit into the silicon baking form for about 10 minutes before removing the silicon baking form. Let the bread cool down on a wire rack! When cold, enjoy with a good slice of cheddar cheese, some lavender honey or whatever you like! 😉 I keep my bread in a bread or larger cookie tin!
Stay tuned, Subscribe by email through the link below to get my new cool newsletters aka posts! Join 1,800 email Subscribers! It is free!
Follow me on Facebook too! 🙂
61 responses to "Gluten-free triple quinoa bread!"
I have never had quinoa in a bread. I cannot even imagine how it may taste or its consistency. Since I do enjoy plain cooked quinoa a lot, I'm sure this bread must be wonderful too.
This looks yummy and I bet it's delicious!
this recipe will be very useful for some of our gluten-free friends! thanks!
Dear Sophie,
great! I bake my own bread for more than 30 years (mixture rye and wheat with sour dough) and was looking for a kind of different, just for a change. I`ll try your recipe – next week, as now I have so much bread left.
We wish you a GREAT evening
Klausbernd and his very busy Bookfayries Siri and Selma
That`s about my bread 🙂
Your quinoa bread looks great Sophie, soft and tender:-)
Are you back to eating gluten free?
Hello Sophie,
Well done to Peter. A few days ago I tasted that famous bread. It was divine!
This looks like it would be perfect slathered with citrus marmalade. I'd wake up to this any day of the week.
Wow Peter! This bread looks amazing…I don't think you could pack anymore quinoa goodness into this if you tried! I love it!
Sophie – this bread looks amazing! Love the quinoa – so healthy too!
Sophie this bread looks delicious…I can't wait to try making it!
Would this be suitable for sandwiches? I have made a quinoa quick bread before that didn't rise at all and was about 1 inch tall after baked. I have been searching hard for a starch-free bread recipe that is more like a loaf than flat bread. I love quinoa, however, quinoa flour is quite expensive. Thanks so much.


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login with
Period pains? (TMI)
I'm a little concerned when I think of pains that deal with periods I think cramping that's all my body has ever done at this time. Well I started a couple days early and all was fine till last night I began having pains not cramps like grabbing my lower abdomen taking deep breaths kinda pain, (the pain is just in the middle of my lower abdomen) I thought maybe it's the tampon, I remove it and I've still had this random pain hit granted it's more painful with a tampon in but this has never happened to me before, I told my husband if the pain lasted longer than it does now I will be goin to the ER! Anyone experience this before!? Just curious what "could"be the issue.
I have never had this happen to me personally, but I do know folks that have had severe cramping with their period. You could try a heating pad? or warm bath? That could help ease the cramping maybe.
I actually wondered about that, with my 2nd child we were ttc for 8 months and when we finally got pregnant the doc explained that according to the date I conceived I was on my period, so I guess it's a possiablity id rather that be the case then something else! Lol
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text: NEW YORK ( TheStreet) -- Constant Contact (Nasdaq: CTCT) hit a new 52-week high Friday as it traded at $32.39 compared with its previous 52-Week high of $32.09. Constant Contact is changing hands at $32.23 with 247,997 shares traded as of 11:16 a.m. ET. Average volume has been 354,800 shares over the past 30 days. Constant Contact has a market cap of $868.7 million and is part of the services sector and media industry. Shares are down 3.5% year to date as of the close of trading on Thursday. Constant Contact, Inc. provides on-demand email marketing and online survey solutions, as well as event marketing solutions for small organizations, including small businesses, associations, and non-profits located primarily in the United States. The company has a P/E ratio of 77.5, below the average media industry P/E ratio of 299 and above the S&P 500 P/E ratio of 23.4. TheStreet Ratings rates Constant Contact as a hold. The company's strengths can be seen in multiple areas, such as its robust revenue growth, largely solid financial position with reasonable debt levels by most measures and impressive record of earnings per share growth. However, as a counter to these strengths, we find that we feel that the company's cash flow from its operations has been weak overall. You can view the full Constant Contact Ratings Report. See all 52-week high stocks or get investment ideas from our investment research center.


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Reverend And The Makers - Silence Is Talking MP3
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From: "Emotional Screening Device" 2002.
Talking Dead - Steven Yeun finally breaks silence MP3
From Talking Dead 5x07 "Heads Up" Scott M. Gimple & Steven Yeun appeared in Talking Dead from Atlanta, Georgia via satellite.
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text:
Identification of differentially expressed genes and pathways for intramuscular fat deposition in
Intramuscular fat (IMF) is one of the important factors influencing meat quality, however, for chickens, the molecular regulatory mechanisms underlying this trait have not yet been determined. In this study, a systematic identification of candidate genes and new pathways related to IMF deposition in chicken breast tissue has been made using gene expression profiles of two distinct breeds: Beijing-you (BJY), a slow-growing Chinese breed possessing high meat quality and Arbor Acres (AA), a commercial fast-growing broiler line.
Agilent cDNA microarray analyses were conducted to determine gene expression profiles of breast muscle sampled at different developmental stages of BJY and AA chickens. Relative to d 1 when there is no detectable IMF, breast muscle at d 21, d 42, d 90 and d 120 (only for BJY) contained 1310 differentially expressed genes (DEGs) in BJY and 1080 DEGs in AA. Of these, 34–70 DEGs related to lipid metabolism or muscle development processes were examined further in each breed based on Gene Ontology (GO) analysis. The expression of several DEGs was correlated, positively or negatively, with the changing patterns of lipid content or breast weight across the ages sampled, indicating that those genes may play key roles in these developmental processes. In addition, based on KEGG pathway analysis of DEGs in both BJY and AA chickens, it was found that in addition to pathways affecting lipid metabolism (pathways for MAPK & PPAR signaling), cell junction-related pathways (tight junction, ECM-receptor interaction, focal adhesion, regulation of actin cytoskeleton), which play a prominent role in maintaining the integrity of tissues, could contribute to the IMF deposition.
The results of this study identified potential candidate genes associated with chicken IMF deposition and imply that IMF deposition in chicken breast muscle is regulated and mediated not only by genes and pathways related to lipid metabolism and muscle development, but also by others involved in cell junctions. These findings establish the groundwork and provide new clues for deciphering the molecular mechanisms underlying IMF deposition in poultry. Further studies at the translational and posttranslational level are now required to validate the genes and pathways identified here.
During the past decades, meat poultry breeding has been predominantly focused on accelerating growth rate and yields of breast and thigh meat. The impressive progress made in these traits, however, has been accompanied by deterioration of taste quality of the broiler meat and, in some markets, decreased acceptability of the meat by consumers
Intramuscular fat (IMF), located in most species in the epimysium, perimysium and endomysium, is an important determinant of meat quality. A certain amount of IMF can enhance meat quality traits such as the flavor, juiciness, water holding capacity and tenderness
Compared to beef, chicken meat does not present a marbled aspect and intramuscular fat is not visible and not anatomically separable; a higher proportion of IMF is represented by polar lipids, presumably within membranes. Previous studies found that the IMF content of chicken meat increased with age from d 28 to d 90
For livestock IMF, studies of the molecular mechanisms underlying IMF deposition have revealed large numbers of DEGs and signaling pathways including ADAMST4-signaling and insulin-signaling pathways
Differentially expressed gene profiles in breasts of slow- and fast-growing chickens
To identify potential candidate genes affecting chicken IMF deposition, gene expression profiles in breast muscle of both Beijing-you (BJY, a slow-growing Chinese breed) and Arbor Acres (AA, commercial fast-growing broiler) chickens at different developmental stages were examined using Agilent cDNA microarray technology. Divergence of breast growth rates in BJY and AA chickens are shown in Table
Additional file 1
Annotation and changing of 1746 common DE genes in AA and BJY chickens.
Click here for file
Figure 1
Numbers of genes that were differentially expressed in breast muscle between days 21, 42, 90 and 120 (BJY) versus day 1.
Numbers of genes that were differentially expressed in breast muscle between days 21, 42, 90 and 120 (BJY) versus day 1. BJY, Beijing-you; AA, Arbor Acres.
Based on the known DE genes, GO analysis was performed in each breed, and the enriched GO-terms (P < 0.05) in the ontology classification "Biological Process" were selected and are presented in Additional file
Additional file 2
Common Enriched GO terms among the differentially expressed genes in both AA and BYJ chickens.
Click here for file
To validate the microarrays, normal distribution analysis was performed with the results of the nine microarrays, and all of their coefficients were <0.5. Based on the 595 known genes that were shared as DEGs in both breeds, cluster analysis of all microarrays was performed (Figure
Figure 2
Cluster analysis of all microarrays.
Cluster analysis of all microarrays. Expression profiles of samples at different ages (1d, 21d, 42d, 90d and 120d marked) of the same breed (AA and BJY marked) were polymerized together; the expression profile of genes at d 1 differed more than those at other ages. BJY, Beijing-you; AA, Arbor Acres.
To further validate the results of microarray testing, q-PCR was used to examine the relative expression of 9 DE key genes selected in each breed at different ages. The results showed acceptable consistency between the results of q-PCR and the fold-change of DE genes in the microarrays (Table
Key genes related to lipid metabolism and muscle development
As IMF is located throughout skeletal muscle, and not as discrete adipose deposits in chicken, it is reasonable to assume that DEGs related to muscle development or lipid metabolism in current study would contribute to its process of deposition. The GO-term analysis showed that known DE genes related to muscle development (34 in BJY, 39 in AA) and genes related to lipid metabolism (59 in BJY, 70 in AA) were found (Additional file
Additional file 3
Key genes related to lipid metabolism and muscle development in BJY chicken.
Click here for file
Additional file 4
Key genes related to lipid metabolism and muscle development in AA chicken.
Click here for file
Additional file 5
Common DE genes related to muscle development or lipid metabolism in AA and BJY.
Click here for file
Genes for which the expression was significantly correlated with changes in muscle development and lipid deposition were analyzed as these genes might be directly involved in IMF regulation. The changes in muscle weight, the content of IMF, triglyceride (TG) and phospholipids (PLIP) in breast tissue of the two breeds at different ages are provided in Table
Based on the trait measurements, relationships between expression of DEGs and muscle development and lipid deposition (IMF, TG, PLIP contents) were examined. The expression of
The relative abundances of
Figure 3
Correlation analysis between the mRNA expression of MYBPC1 (Myosin binding protein C, slow type), GLTPD1(Glycolipid transfer protein domain containing 1), CETP (Cholesteryl ester transfer protein) or SNX4(Sorting nexin 4) genes determined by q-PCR and the breast muscle weight, PLIP, IMF or TG contents in BJY or AA chickens.
Correlation analysis between the mRNA expression of MYBPC1 (Myosin binding protein C, slow type), GLTPD1(Glycolipid transfer protein domain containing 1), CETP (Cholesteryl ester transfer protein) or SNX4(Sorting nexin 4) genes determined by q-PCR and the breast muscle weight, PLIP, IMF or TG contents in BJY or AA chickens. The r value indicates Spearman's Correlation. IMF, Intramuscular fat; TG, Triglyceride; PLIP, Phospholipids; BJY, Beijing-you; AA, Arbor Acres.
Pathways and a regulatory network for IMF content in chickens
The regulation of IMF is possibly a function of complex pathway interactions involving muscle, fat and connective tissue
Additional file 6
Identification of enriched KEGG pathways based on known DEGs related to muscle development and lipid metabolism in AA and BJY chickens.
Click here for file
Figure 4
Proposed regulatory network for chicken IMF based on significantly different GO terms and KEGG pathways.
Proposed regulatory network for chicken IMF based on significantly different GO terms and KEGG pathways. This network is involved in several cellular functions including lipid metabolism (pathways for ErbB, MAPK & PPAR signaling) and cell junction (tight junction, ECM-receptor interaction, focal adhesion, regulation of actin cytoskeleton). Whether or not cell junction related pathways function on IMF deposition through lipid metabolism related pathways (dotted lines) needs further study. FGF & FGFR, Fibroblast growth factor and its receptor; PPAR, Peroxisome proliferator-activated receptor; ECM, Extracellular matrix.
cDNA array analysis
Fat deposition in chickens takes place mainly in visceral adipose tissue and in muscle. The latter, IMF, is very important for sensory aspects of meat quality and there is increasing interest in improving quality, perhaps using marker-assisted selection for IMF. Although global gene expression surveys have been performed on visceral tissues
Chicken microarrays were employed, each using pooled RNA samples (n = 6 birds, within each of two breeds and at 4 or 5 ages; 9 arrays in all). Such a pooling strategy can dramatically improve accuracy when only one array is available in each biological condition
Figure 5
Technical validation of microarray results by q-PCR by correlation: (a) BJY and (b) AA chickens.
Technical validation of microarray results by q-PCR by correlation: (a) BJY and (b) AA chickens. The r value indicates Spearman's Correlation between the two methods.
Function of key DEGs affecting IMF and muscle development
The data obtained here indicate that three key genes related to lipid metabolism were shared by the two breeds (
For key candidate genes affecting muscle development, one gene common to the two breeds (
Function of novel pathways related to IMF
GO-term analysis was used to explore the function of DEGs and KEGG pathway analysis was used to explore the regulatory network underlying chicken IMF deposition. As expected, several well-known pathways related to lipid metabolism were found, including the MAPK and PPAR signaling pathways
Of special interest, these GO and KEGG analyses provide the first demonstration that a series of pathways related to cell junctions might contribute to the deposition of IMF. DEGs related to muscle development included well-known genes (
The KEGG analysis implicated the MAPK signaling pathway in processes involving tight junctions, focal adhesion and regulation of the actin cytoskeleton. This is also consistent with studies showing that activation of MAPK activity resulted in the disruption of tight junctions, and that inhibition of MAPK activation prevented this process
The present approach has used gene expression profiling to elucidate molecular mechanisms of post-hatch IMF deposition in chickens. Possible regulation by translational mechanisms and posttranslational modifications may also contribute. A more complete understanding of IMF development in chickens should include further examination of the expression and function of the proteins encoded by the genes identified here in both embryonic and post-hatch stages of development.
With aim of identifing global candidate genes and new pathways related to IMF deposition in chicken breast, Agilent cDNA microarray analyses were performed with both fast- and slow- growing breeds. Gene expression profiles of breast muscle sampled at different developmental stages of BJY and AA chickens were determined. Relative to d 1, breast muscle at d 21, d 42, d 90 and d 120 (only for BJY) contained 1310 DEGs in BJY and 1080 DEGs in AA. Several DEGs (
All experimental procedures, using female Beijing-You chickens (BJY, the Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China) and Arbor Acres (AA, Dadongliu broiler Company, Beijing, China), were performed in accordance with the Guidelines for Experimental Animals established by the Ministry of Science and Technology (Beijing, China). Individuals within each breed had the same genetic background. Birds (60 BJY and 48 AA) were reared in stair-step caging under continuous lighting using standard conditions of temperature, humidity and ventilation. Chickens used for sample collection at d 1 were not fed. The same diet was fed to all chickens and was formulated to be intermediate between recommendations for the two breeds
Sample collection
At each sampling age, d 1, d 21, d 42, d 90, and d 120 (only for BJY), six birds of similar weight from each breed were sacrificed for tissue collection. Samples of the left
Trait measurements
Intramuscular fat (IMF) content of breast muscle was determined by extraction with petroleum ether in a Soxhlet apparatus
Samples of the right
Total RNA preparation and microarray hybridization and analysis
Total RNA was isolated from breast muscle samples using Trizol reagent (Invitrogen, USA) according to the manufacturer's instructions and dissolved in RNase-free water at a final 2.0 μg/μl concentration. RNA from
The normal distribution of signal plots in every chip was determined. Clustering was performed based on the DE genes in each chicken breed, using un-centered Pearson correlations and average linkage cluster 3.0, and was displayed in TreeView. Normalized fluorescence intensity values of each dye-swapped experiment were averaged separately for sample and reference channels. Thereafter, for each probe, averaged sample and reference fluorescence values were log2-transformed. Average linkage hierarchical clustering was performed using the Euclidian metric. In heat-maps, the color of features (probes) was determined by log2 (reference/sample).
Analysis of gene expression profile and differentially expressed genes
The distribution of expressed genes was analyzed by JMP4.0 according to their expression level. If the flag of a gene was "A" by the scanner according to the data normalization and results of Agilent Microarray Suite 4.0 software, it was considered to be "not detected", and hence "not expressed" in this study. Similarly, the genes with "P" flags were considered to be "expressed transcripts". Expressed transcripts were defined as being present in samples of at least one sampled age and were used for all following studies. The expression value of each probe set was normalized and calibrated using the RMA method.
Screening of differentially expressed genes (DEGs) was performed on the basis of differences in the IMF, TG and PLIP contents at different ages in each breed. Expression at d 1 was used as the controls, and comparisons were made within each breed at d 21, 42, 90 and 120 (only for BJY). Genes were considered to be DEGs only when the fold-change in abundance for all comparisons exceeded 2.0.
Quantitative real time RT-PCR (q-PCR)
To avoid amplification of any residual genomic DNA, all PCR primers were placed at or just outside the exon/exon junctions and specificity was determined with BLASTN (Additional file
Additional file 7
Selected qPCR primer sequences and accession numbers.
Click here for file
Quantification of the transcripts was determined using standard curves with 10-fold serial dilutions of cDNA (10-7 to 10-12 g). Melting curves were constructed to verify that only a single PCR product was amplified. Within runs, samples were assayed in triplicate with standard deviations of threshold cycle (CT) values not exceeding 0.5, and each q-PCR run was repeated at least twice. Negative (without template) reactions were performed within each assay.
Gene ontology enrichment analysis and visualization
Gene Ontology enrichment analysis was performed for features corresponding to DEG in each breed using the GOEAST software toolkit. The significance level of GO term enrichment was set as FDR-adjusted p-value less than 0.05 by the Yekutieli method.
Screening of the key DE genes
Correlation analysis was performed between the key DE genes related to muscle development or lipid metabolism with changes in lipid content or breast muscle weight across the sampled ages within each breed. If the expression of a given gene was significantly correlated (p < 0.05) with breast tissue weight, or content of IMF, TG or PLIP, this gene was considered to be a key DE gene.
KEGG pathway analysis
KEGG pathway
ACSF3, Acyl-CoA synthetase family member 3; ACSL1, Acyl-CoA synthetase long-chain family member 1; ACSS(1&2), Acyl-CoA synthetase short-chain family member; ACTB, Actin, beta; ADAMST4, A disintegrin and metalloproteinase with thrombospondin motif 4; ANKRD1, Ankyrin repeat domain 1; APOH, Apolipoprotein H; BMP(4,7), Bone morphogenetic protein; BMP2K, BMP-2-inducible protein kinase; CAMs, Cell adhesion molecules; CAPNS1, calpain, small subunit 1; CD36, Cluster of Differentiation 36; CD44, CD44-like protein; CENPF, Centromere protein F; CETP, Cholesteryl ester transfer protein; CH25H, Cholesterol 25-hydroxylase; CTNNB1, Catenin (cadherin-associated protein), beta 1; CYP17A1, Cytochrome P450, family 17, subfamily A, polypeptide 1; DCK, Deoxycytidine kinase; DGAT2, Diacylglycerol O-acyltransferase homolog 2; ECM, Extracellular matrix; EGF, Epidermal growth factor; EHHADH, Enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; ELN, Elastin; ErbB, Erythroblastic Leukemia Viral Ongene Homolog; ETFDH, Electron-transferring-flavoprotein dehydrogenase; FABP(13, 5, 6, A/Adipocyte, H/Heart), Fatty acid binding protein; FGF(47, 16), Fibroblast growth factor; FGFR, Fibroblast growth factor receptor; GDF3, Growth differentiation factor 3; GLTPD1, Glycolipid transfer protein domain containing 1; HMGCLL1, 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1; KEGG, Kyoto Encyclopedia of Genes and Genomes; LDLR, Low density lipoprotein receptor; LOC416618, NAD(P) dependent steroid dehydrogenase-like,; MAP2K3, Mitogen-activated protein kinase kinase 3; MAPK, Mitogen-activated protein kinase; MBOAT2, Membrane bound O-acyltransferase domain containing 2; MYBL2, V-myb myeloblastosis viral oncogene homolog (avian)-like 2; MYBPC1, Myosin binding protein C, slow type; MYCN, V-myc myelocytomatosis viral related oncogene, neuroblastoma derived; MYF5, Myogenic factor 5; MYH(4, 6, 7B), Myosin, heavy polypeptide; MYL(23, 4, 10), Myosin, light chain; MYO15A, Myosin XVA; NACA, Nascent polypeptide-associated complex alpha subunit; NR3C2, Nuclear receptor subfamily 3, group C, member 2; PGK1, Phosphoglycerate kinase 1; PLTP, Phospholipid transfer protein; PLG, Plasminogen; PPAR, Peroxisome proliferator-activated receptor; PPARGC1A, Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; RBP7, Retinol binding protein 7; RCJMB04_13o20, NSFL1 (p97) cofactor (p47); RXRA, Retinoid X receptor-alpha; SGPL1, Sphingosine-1-phosphate lyase 1; SH3PXD2B, SH3 and PX domains 2B; SNX(34, 17, 30), Sorting nexin; TGFA, Transforming growth factor, alpha; THBS1, Thrombospondin-1; THRSP, Thyroid hormone responsive, SPOT14 homolog; UCP3, Uncoupling protein 3; YWHAH, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; WNT4, Wingless-type MMTV integration site family, member 4.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
HXC contributed to the design and performing of the study, the interpretation of data, and writing of the manuscript. RRL contributed to the design of the study, interpretation of data and writing of the manuscript. GPZ contributed to the design of the study, and reviewing of the manuscript. MQZ and CJL contributed to the design of the study and assisted in animal handling. JW designed the study and was in charge of the overall project. All authors submitted comments on drafts, and read and approved the final manuscript.
The authors would like to acknowledge W. Bruce Currie (Emeritus Professor, Cornell University) for his assistance in preparing the manuscript. The research was supported by grants: the project of state key laboratory of animal nutrition (2004DA125184G1101); the National High-tech R&D Program (Grant Number: 2011AA100301); the earmarked fund for modern agro-industry technology research system (CARS-42).
Cellular signaling pathways regulating the initial stage of adipogenesis and marbling of skeletal muscle
Effect of selection for improved body composition on muscle and meat characteristics of broilers from experimental and commercial lines
Proceeding of the 32nd European Meeting of Meat Research Workers
Variations in composition and palatability traits and relationship between muscle characteristics and palatability in a random sample of pork carcass
The eating quality of pork: 2. The influence of intramuscular fat
Influence of intramuscular fat content on the quality of pig meat-1. Composition of the lipid fraction and sensory characteristics of m. longissimus lumborum
Poultry Meat Science
Associations of heart and adipocyte fatty acid binding protein gene expression with intramuscular fat content in pigs
Studies on the characteristics of deposition of chicken IMP and IMF
Development rule of intramuscular fat content in chicken
Review of the factors and genes on intramuscular fatty acid
Skeletal muscle specific genes networks in cattle
Use of a bovine genome array to identify new biological pathways for beef marbling in Hanwoo (Korean Cattle)
Gene expression patterns during intramuscular fat development in cattle
Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
Comparative studies of skeletal muscle proteome and transcriptome profilings between pig breeds
Comparative Analyses by Sequencing of Transcriptomes during Skeletal Muscle Development between Pig Breeds Differing in Muscle Growth Rate and Fatness
In vivo cholesterol and fatty acid synthesis in the pig intestine
Glycogen metabolism in meal-fed rats and chicks and the time sequence of lipogenic and enzymatic adaptive changes
In vitro hepatic lipogenesis in the hen and chick
Some differences between avian and mammalian biochemistry
Profiling of chicken adipose tissue gene expression by genome array
Microarray analysis of differential gene expression in the liver of lean and fat chickens
Systematic identification of genes involved in divergent skeletal muscle growth rates of broiler and layer chickens
Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers
On the utility of pooling biological samples in microarray experiments
de la Luz Sierra M, Barr VA, Haft DH, Taylor SI: Identification of a family of sorting nexin molecules and characterization of their association with receptors
Glycolipid transfer proteins and membrane interaction
Slow-type C-protein in dystrophic chicken fast pectoralis muscle
Intercellular signaling between adipose tissue and muscle tissue
Cloning of a rat adipocyte membrane protein implicated in binding or transport of long-chain fatty acids that is induced during preadipocyte differentiation. Homology with human CD36
The multigene family of fatty acid-binding proteins (FABPs): function, structure and polymorphism
The role of plasma lipid transfer proteins in lipoprotein metabolism and atherogenesis
Gene expression of heart- and adipocyte-fatty acid-binding protein and correlation with intramuscular fat in Chinese chickens
Associations of A-FABP and H-FABP markers with the content of intramuscular fat in Beijing-You chicken
From cadherins to catenins: cytoplasmic protein interactions and regulation of cell adhesion
Molecular and functional analysis of cadherin-based adherens junctions
The roles of FGFs in the early development of vertebrate limbs
Diversity of function is inherent in matricellular proteins: an appraisal of thrombospondin 1
CD36: implications in cardiovascular disease
ADAM12 induces actin cytoskeleton and extracellular matrix reorganization during early adipocyte differentiation by regulating beta1 integrin function
Regulation of fibronectin, integrin and cytoskeleton expression in differentiating adipocytes: inhibition by extracellular matrix and polylysine
Decreases in tubulin and actin gene expression prior to morphological differentiation of 3 T3 adipocytes
Functional analysis of mitogen-activated protein kinase-3 (MAPK3) and its regulation of the promoter region in zebrafish
Defective thymocyte maturation in p44 MAP kinase (Erk 1) knockout mice
Feeding standard of chickens, ICS 65. 020. 30, B 43, NY/T 33–2004
Estimation of genetic parameters for fat deposition and carcass traits in broilers
Sloane Stanley GH: A simple method for the isolation and purification of total lipids from animal tissues
Practical methods for plasma lipoprotein analysis
Hetercrogeneity of human serum high density lipoproteins on high performance liquid chromatography
New enzymatic assay for calcium in serum
KEGG: kyoto encyclopedia of genes and genomes
From genomics to chemical genomics: new developments in KEGG
KEGG for linking genomes to life and the environment


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Doctor insights on:
Split Lip
Should i put ice on a split lip?
Sure: Not only will the ice reduce swelling from the traumatic cause of the split, but the cold constricts blood vessels and greatly reduces or stops the bleeding. And it feels good! ...Read more
Mouth (Definition)
Mouth (mouth) " n. Pl. Mouths 1. A. The body opening through which an animal takes in food. B. The cavity lying at the upper end of the alimentary canal, bounded on the outside by the lips and inside by the oropharynx and containing in higher vertebrates the tongue, gums, and teeth. C. This cavity regarded as the source of sounds and speech. D. The opening to any cavity or canal ...Read more
What can be done about a split lip?
Split lip: A split lip needs to be repaired by a doctor, particularly if it is deep enough to be into the muscle of the lip. This is done with sutures that can be dissolvable in most cases. A surface abrasion will heal on its own and can be treated with antibiotic ointment. ...Read moreSee 5 more doctor answers
How to help swollen, split lips. They hurt.
Help 4 swollen lips: I would not do what ever made them sore anymore. 1. Too much kissing can do it. But if split either it was way too much kissing or you are dehydrated. 2. Can also be due to allergy to meds or cat or shrimp etc with severe reaction so need to go to urgent care to see what else is going on. Unless 1 is the cause. Keep us posted. ...Read more
Child split lip will require stitches or no?
Maybe,maybe not: Generally, if the cut is limited to the red of the lips, it is ususlly left open to self heal.Bacteriostatic factors in saliva keep it from festering. If the cut crosses over into the junction between facial skin and the red , stitches may be needed to line up the edges and prevent healing in a jaged line. ...Read more
How to help swollen, split lips. They hurt.
5 months pregnant and just started.
Petroleum jelly: Many product like Vaseline / Chap-stick have moisturizer that will help. Both are safe while pregnant. ...Read more
Passed out monday, hit my head on the ground. Black eye, split lip and lump on my head. Headaches and sensitivity to smell. Go to my pcp? Or wait
Yes, PCP, or ED: Passing out and hitting your head with a new change in smell, as well as headaches deserves an evaluation. Your split lip may need stitches too. I recommend going your pcp or your local emergency department to review your symptoms and injuries. Passing out syncope) requires a work up. ...Read more
I split my lip open lifiting up a bicycle and it is pretty deep can I use liquid bandaid?
No: Generally liquid band aide does not work on wet reas. Plus there are many muscles in the lip, so i would have it evaluate. ...Read more
Simon split his lip last night and it is swollen and an angry red now. What can I do to make the swelling go down and know it's not infected?
Too early: Rarely would a worrisome infection occur overnight. Ice packs and watch and wait for now. RJ ...Read more
My 1 yr old split his lip last night and it is swollen and an angry red now. What can I do to make the swelling go down and know it's not infected?
Lip: Applying a cool cloth may help or giving him a Popsicle. Make sure his teeth are not affected. If the swelling is looking worse instead of better in 1-2 days, have him seen. All the best. ...Read more
The center part of my lower lip split and is bleeding nonstop for 20 minutes with pressure applied. Whatshould I do?
Go to ER/see Dr now: You're going to need stitches in that lip. ...Read moreSee 3 more doctor answers


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DC Coupled means eurorack friendly!
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Bacon 5 GF
Kids' Dinner Menu
10 & under / Served with choice of mini salad, housemade applesauce OR sautéed broccoli
Turkey Burger 6
Housemade ketchup, cheddar, provolone, pickles, birdseed bun
Battered Walleye 6
Red Lake walleye, housemade tartar sauce
Black Bean Quinoa Burger 6
Housemade ketchup, cheddar, provolone, pickles, birdseed bun
Hot Dog 5
Ferndale Market Turkey hot dog, fresh baked bun & housemade ketchup
Cheese Pizza 6
Tomato fennel ragout, cheddar, provolone / Available with broccoli by request
French Toast 5
Maple syrup, powdered sugar
Mixed Berry Crisp GF (V)
Key Lime, Strawberry Key Lime, Cherry, Coconut Cream Pie
Poppyseed Cake with blueberry lemon curd
Chocolate Carrot Cake with cream cheese frosting
Strawberry Shortcake
Bakery Case / Ask your server for today's selections
Izzy's Ice Cream Single Scoop 4 / Double Scoop 5
Sweet Science Sorbet Single Scoop 5 / Double Scoop 7