text:
All-Star balancing act: Players vs. integrity
PHOENIX Rule No.1 for All-Star Game managers was demonstrated again Tuesday: Protect the players in your care.
It makes sense. So pitchers who started for their teams Sunday couldn't play. Boston Red Sox pitcher Josh Beckett felt knee soreness warming up in the first inning and shut it down. Detroit Tigers first baseman Miguel Cabrera felt a pull in his side muscle on a swing and left one inning later. (He said he probably would have stayed in if it were a regular-season game).
It is, after all, an exhibition game. Both managers worked hard to get everybody in — 60 players appeared in the National League's 5-1 victory, a record for a nine-inning game.
But as the new rules to protect players have evolved, have we forgotten about protecting the integrity of the World Series? This game decides which league gets home-field advantage for the Fall Classic.
BLOG: NL Breakdown
BLOG: AL Breakdown
PHOTOS: 2011 All-Star weekend in pictures
So this year's game counted but also showed the flaws in trying to have it both ways.
Even before Beckett was scratched, the American League pitching staff was missing All-Stars Justin Verlander, Felix Hernandez, CC Sabathia and James Shields because they started Sunday, a rule instituted last year. Would the game have been different with them available? It's almost a rhetorical question.
"You never know what really would have happened if they had some of their best pitchers," Cincinnati Reds second baseman Brandon Phillips said. "All I can do is say thank you very much for not pitching. … It probably would have been a different game."
Players didn't criticize the New York Yankees' Derek Jeter or Mariano Rivera for skipping the game to rest minor injuries, even though they played last weekend. Some fans would claim poetic justice if it ultimately forced them to play a Series Game7 on the road.
"He needs to gets his treatment, and he needs to rest," Yankees second baseman Robinson Cano said of Jeter. "I know I need my captain. For the season, not here."
No one is suggesting the players don't play hard in the All-Star Game. Jose Bautista's sliding catch of a foul pop in the second demonstrated that. But the Toronto Blue Jays outfielder knows there are sensible limits, as he showed when thrown out at the plate in the fourth.
"You don't see people running over people at the plate like Pete Rose, but it's still a competitive game," said Los Angeles Angels pitcher Jered Weaver, the AL starter who was limited to one inning on orders from his manager, Mike Scioscia.
The home-field advantage rule came as a result of the tie game in Milwaukee in 2002, and Chicago White Sox first baseman Paul Konerko says it has made a difference in the competitiveness of the game.
"I was in the Milwaukee game. That was my first one, and it definitely felt like an exhibition. You could tell nothing was at stake," said Konerko, in his fifth All-Star contest. "All the other ones I've been in since then, there's definitely a feeling that we want to win the game."
But Konerko and other players say they don't like the rule.
"I'm not a huge fan, to be honest, of the home field being decided by the All-Star Game," he said. "It's weird that somebody who is on a last-place team right now could have an effect on that in this game."
The dilemma was exemplified by San Diego Padres reliever Heath Bell, who made a running slide as he arrived at the mound in the eighth. "I told some guys I wanted to have fun this All-Star Game and needed some ideas, so guys back home kind of said slide on the mound," he said, adding, "(NL manager Bruce Bochy) said before the game that this really counts, so I thought I was not going to do it. But then we were up by four runs."
Posted | Updated


text:
CD/MP3/WAV Player with Graphical Spectrum Analyzer and Synchronized Visuals
Use this security logo:
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hypnogenic, Analyzer, WAV, CD Spectrum Pro, Visuals, Graphical, psychedelic, Media Player, MP3, kinemorphic
Minor Update
Distribution: This software may be freely distributed in any format provided that it is remains unmodified and it is made clear that it is shareware.
CD / Spectrum Pro
License Agreement
CD/Spectrum Pro
Copyright (C) 1996-2001
All Rights Reserved
By John Hornick
Email: email@example.com
Definition of Shareware - what is shareware?
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Definition of Shareware
Shareware distribution gives users a chance to try software before
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right to continue using the software to an updated program with
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Copyright laws apply to both Shareware and commercial software, and
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author specifically grants the right to copy and distribute the
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Shareware is a distribution method, not a type of software. You
should find software that suits your needs and pocketbook, whether
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Users of 'CD/Spectrum Pro' must accept this disclaimer of warranty:
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is a "shareware program" and is provided at no charge to the user
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expected to use the software on your machine and evaluate its
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If you find this program useful and find that you are using
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subset: commoncrawl


text:
Vegas Lit is a new fiction imprint of Huntington Press in Las Vegas. Arnold Snyder is the senior editor. Vegas Lit seeks to publish first-rate literary fiction or genre fiction that transcends its category.
"Writing nonfiction is an intellectual exercise, while writing fiction is an emotional experience."
Q: You are the senior editor of Vegas Lit, a new fiction imprint of Las Vegas publisher Huntington Press. Tell us about this exciting new venture and your role in it.
A: I'm really excited about Vegas Lit. As senior editor, I get to choose the books we publish and I personally work with the authors, which is something I greatly enjoy. I've been writing and editing nonfiction for more than thirty years and this opportunity to work with fiction writers was something I couldn't pass up.
All of the best fiction today is coming out of the small presses. The big New York publishing giants are cranking out predictable, boring books. They take very few chances. Their industry is dying, but that doesn't mean the audience for books is dying. That's a myth. Look at the data sometime for the number of ebooks being published, being sold, being read. There are more readers hooked on books today than ever and this is one of the most exciting times to work in this industry.
Ten, twenty years ago, it cost an arm and a leg to publish a book, print a book, distribute a book. Each title took a huge investment. All serious writers needed agents if they expected to get anywhere. Small presses existed, but they were like voices crying in the wilderness. They couldn't get distribution. People didn't know they existed. Technology has changed that. There's a literary revolution taking place and it's exciting to play a part in it.
Q: Cover designs for books published by Vegas Lit are provided by local Las Vegas artists. Why did you choose to find covers this way?
A: Hey, just go gallery hopping in downtown Vegas on the first Friday of any month and you'll know the answer to that question. In the past ten years, this town has become a mecca for artists. There's no other city like Las Vegas. The thousands of show people who live in this town, and those who do the design work and costuming and lighting, not to mention the musicians—there's just a high percentage of talented artists of all types here. And creative people tend to be very tolerant of diversity. Artists are drawn to tolerant places and it's probably significant that it's also relatively cheap to live here, compared to say, New York, L.A., or San Francisco.
The first two Vegas Lit titles have cover art by Joseph Watson. The third one that comes out in the spring will have cover art by Montana Black. I first saw the work of both artists in their downtown galleries.
Q: You are not only the senior editor, but also the inaugural author with the Vegas-based novel, Risk of Ruin. Tell us about it.
A: At the age of fifteen, in 1963, I read Jim Thompson's The Grifters. That novel inspired me to become a writer. What I liked about Thompson's novels was that there were no good guys. Every character had a dark side, but still, you found yourself rooting for and caring about the bad guys.
My narrator/protagonist in Risk of Ruin is an outlaw biker, professional gambler, sometime drug dealer—not your normal leading man. It's a challenge to get the reader to relate to a character like this, but that's why I write. It's boring for a writer to make his protagonist tall, dark, handsome, brave, clean, and reverent. And it's bullshit. Real people aren't like that. Everyone has a dark side, a crazy side, a mean side, a violent side, a stupid side, a spiritual side. My protagonist is not a very nice guy or even a very sociable guy a lot of the time. He's a forty-three-year-old scooter tramp, angry at the world and his lot in life, who becomes obsessed with an underage girl who's working at a strip club using a fake ID.
When I started writing this book, I talked about the idea with another writer. I told him it was basically a love story about a social renegade in his forties who has a love affair with a runaway teenage girl. He told me it was a terrible idea to write a book like that, because the concept itself would turn people off so much so that no one would read it. He said the guy would come off creepy, no one would see it as a love story, and the best I could hope for would be that it would be taken as some kind of psychological study of a pervert, sort of like Nabokov's Lolita. That made the challenge even greater and I think I succeeded in what I was trying to do. Risk of Ruin is a love story. It just has a bit more violence and desperation and far less typical characters than most love stories.
Q: You have an impressive resume of over a dozen nonfiction books on casino games and gambling published over the years. How is writing fiction similar to nonfiction? What are the differences from your perspective?
A: In fiction, everything comes out of the author's head. This gives the author incredible freedom, but he can also get stuck. His characters come alive and may not want to go where he'd planned to take them. He's got to wait until his characters take him where they want to go. Writing nonfiction is an intellectual exercise, while writing fiction is an emotional experience.
Q: Many people living in Las Vegas can't wait to leave, while others can leave yet the lights won't let them. How is your relationship with Vegas?
A: I love Vegas. It's an honest place. It's about sex and money and greed and fun and kicking back and letting go and regretting, if you must, tomorrow. But today, let's party. It's a healthy attitude for people in this society, especially today as the whole world appears to be changing faster than any of us can keep up with it, and a lot of the changes aren't for the better. There's an intense energy in this town. It's a town full of dreamers and schemers and people who take chances.
Q: Where do you see Las Vegas in ten years?
A: Eight years from now, the Republicans will get the country back. One of the first things they'll do is start sending the nuclear waste to Yucca Mountain. They've got hundreds of millions invested in that mountain already and the only reason the country's nuclear waste stockpiles aren't already being dumped in Yucca is Harry Reid, and everyone knows it. He'll be gone. And within a year of the opening of Yucca Mountain, the Las Vegas casinos will start making book on when there will be a radioactive leak or spill.
Then, there's the water issue—will we at last be able to steal the Great Basin water, and how long will it last in the Big Drought that's brewing?
And if the water issue's resolved and we aren't nuked out of this state, Las Vegas is vulnerable to the price of oil. If people can't travel to Vegas cheaply, this town is in for hard times. If the cost of flights and bus tickets and a fill-up at Chevron goes through the roof, we're in trouble. We'll start to see boarded-up casinos. I see that as a realistic possibility within ten years.
Nevada is a state filled with ghost towns and I imagine Las Vegas will shrink back to dust someday. But as long as I can personally get my bets down on the nuclear disaster sweepstakes, I'll be happy. I'm a gambling man, so just give me something to bet on.
Arnold Snyder is a high-stakes professional gambler who has been writing about casino games for over two decades. He was the publisher and editor of Blackjack Forum, a quarterly journal for professional gamblers, from 1981 through 2006. His books The Blackjack Formula, Blackbelt in Blackjack, and Poker Tournament Formula 1 and 2 each challenged the conventional wisdom on optimal strategy for beating these games. In January 2002, Snyder, who is one of the great legends of blackjack, was elected one of the seven charter members of the Blackjack Hall of Fame. Snyder's 2012 novel Risk of Ruin is the first of a planned series featuring characters who are professional gamblers. His blog, Write-aholic, contains reviews and essays of fiction and other writing-related topics.


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text:
Your Evening Jemmy
But how easily are base and
selfish measures, masked by pretexts of public good and
apparent expediency? How frequently will a repetition of
the same arts and industry which succeeded in the first
instance, again prevail on the unwary to misplace their
confidence? How frequently too will the honest but unenlightened
representative be the dupe of a favorite leader, veiling his
selfish views under the professions of public good, and
varnishing his sophistical arguments with the glowing colours
of popular eloquence?
Advertisement - Continue Reading Below
-- James Madison, Vices Of The Political System Of The United States, April, 1787
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text:
Research Associate, Stockholm Environment Institute
Måns Nilsson, Deputy Director and Director of Research, SEI
Marion Davis, Communications Manager, SEI's U.S. Centre
A "nexus" approach to addressing water, energy and food security may prove a useful model for developing the SDGs.
The world has made great strides in reducing poverty and improving living conditions since the Millennium Development Goals were set two decades ago. Now, with the Sustainable Development Goals, ambitions are even higher: Not only do world leaders want to keep fighting poverty, hunger and disease, but they want to ensure human prosperity and well-being for the long term. This means engaging all countries – rich and poor – in the quest for a more socially and environmentally sustainable future.
Given the SDGs' broad mandate, it's not surprising that while the MDGs comprised eight goals, the Open Working Group on SDGs has identified 19 focal areas: not just "basics" such as poverty eradication, food security, energy, and water and sanitation, but also higher-level goals such as decent work for all, equality, sustainable consumption and production, peaceful societies and capable institutions.
The list highlights the wide range of social, cultural, economic and political conditions needed to enable sustainable development. It also makes it clear that even the richest countries need to do better. But logistically, it poses a daunting challenge. In the MDGs, each goal is supported by targets identified by experts in that particular field: water, education, health, etc. But with little dialogue across sectors, efforts to achieve the MDGs have sometimes worked at cross-purposes, or competed over resources. Still, early SDGs proposals have followed the same sector-by-sector pattern. Without a better approach, and with 19 focal areas, the SDGs risk becoming a tangled wish list of goals and targets that no one can fully make sense of.
There is another way, and it is gaining momentum in the SDGs talks, driven in part by the Government of Colombia's proposed "Integrating Approach". Rather than come up with separate sets of targets in each focal area, the idea is to organize cross-sectoral dialogues to come up with targets "from the bottom up", thinking in terms of development priorities. Participants can then identify shared interests and examine interactions between targets: Do they compete for the same resources? Do they support or depend on one another? Does one impose constraints on the other? Are additional targets needed to ensure equitable access to resources, and efficient and sustainable resource use?
In February, in preparation for an IRF retreat, we examined how an integrated approach could work in the context of water, energy and food-related targets, using the "nexus" framework as a guide. A growing body of research shows that water, energy and food security are closely interrelated, and SEI and others are using the nexus approach to address development and resource-use challenges around the world.
We started by simply reviewing the water-, energy- and food-related targets identified in four SDG proposals, and marking the ones that might be relevant, or connected, to targets in the other two areas. For example, ensuring safe water quality also relates to food security; similarly, recycling or treating all municipal wastewater would require energy. We found most targets were linked to other sectors.
Second, we examined the nature of cross-sectoral interactions. Some targets are interdependent; for example, increasing access to irrigation requires a steady supply of freshwater. Other targets impose conditions or constraints on others; for example, the target for efficient agricultural water use sets a condition for how access to irrigation can be provided. Some targets reinforce each other, highlighting potential synergies; for example, increasing water efficiency in agriculture could boost agricultural productivity. Finally, critical trade-offs and conflicts may occur – for instance, when food and energy production compete for the same water resource, and the expansion of one impedes the other.
Our third level of analysis focused on resources and conditions as enablers of development. For example, energy is needed for water pumping, and water is needed for both hydropower and thermal power production. Similarly, agricultural production depends on energy and water. And water, energy and food, in turn, are enablers for social sectors. Energy is also crucial to the attainment of both health and education targets, for example: to provide electricity for clinics and schools, and light at home to do homework. Similarly, clean water and sanitation services are also essential to achieving health targets.
The IRF retreat made it clear that there is broad support for an integrated SDGs framework, but also that it is seen as a huge and difficult task. We were pleased to hear that the nexus approach helped participants start making a "mental map" of the linkages. Still, more work will be needed to fully understand those linkages – and their implications: Should the existence of a trade-off or synergy affect whether a target is included or excluded in the SDGs? Should targets that are linked to many other targets be given priority? These are questions that will require not only deeper research and analysis, but also value judgements.
If the SDGs are to succeed, they need to be coherent, consistent and well integrated. A nexus approach alone cannot accomplish that, but it may provide a helpful start. We will keep developing our analysis, and look forward to a lively and productive debate on SDGs integration.
Read the SEI discussion brief: Cross-sectoral integration in the Sustainable Development Goals: a nexus approach.
The blog contains the authors' personal views and does not represent the view of IRF2015. IRF2015 accepts no liability for your use of or reliance on information found on the blog. IRF2015 does not edit and is therefore not responsible for any comments, but reserves the right to review/remove any comment at any time. If you wish to report a comment for any reason, please contact us or flag the comment on the comments system.


text: North Carolina NAACP President Rev. William Barber, accompanied by Rep. Sheila Jackson Lee, Texas, left, as activists, many with the clergy, are taken into custody by U.S. Capitol Police on Capitol Hill in Washington, July 13, 2017, after protesting against the Republican health care bill. AP Photo/J. Scott Applewhite July 14, 2017 Why health savings accounts are a bust for the poor but a boost for the privileged Simon Haeder, West Virginia University The latest Senate health care bill is still a hodgepodge of efforts to repeal Obamacare, critics say. One of their concerns is the focus on HSAs.


text:
Last game vs. Georgia: 23-20 loss
Series Record: Georgia leads 8-3
Most memorable Georgia win: 2002 SEC Championship game – David Greene is named the MVP, and Georgia wins its first SEC title in 20 years.
Most painful Georgia loss: 1969 Sugar Bowl – Georgia falls to Arkansas after winning the SEC title. It was the Bulldogs' only loss of the season.
Starters returning: 11
Best player: Jonathan Luigs
Biggest Weakness: Transition of coaching staff
2008's big question: Can the Hogs stay out of the cellar in the West?
Legge's must-win game: September 20 vs. Alabama
Legge's projected SEC finish: 6th SEC West
Legge's All-SEC Hogs: Jonathan Luigs


text:
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Summer break is painful.
Sizzling is a poor choice of words to decribe the 76 season, makes me think of Niki's unfortunate ears...


text:
About the Book
A Safari to Africa... The wonderful world of the animals just opened up. Lions, leopards, elephants & so many more.
Please share my joy and my images of a trip of a life-time.
I cannot wait to return... And I wil!
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text:
|Specific References
|Related Products
|ERK, Erk2, MAPK2, PRKM2, Prkm1, C78273, p41mapk, p42mapk, AA407128, AU018647, 9030612K14Rik, Mapk1
|Verified forward and reverse primers for analyzing the quantitative expression of gene
|The primer mix has been verified to generate satisfactory qPCR data on Roche LightCycler480
|1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions) is shipped at ambiente temperatura.
|The lyophilized product is stable for one year from date of receipt when stored at -20℃.
The suspended product is stable for six months from date of receipt when stored at -20℃.
Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. ERK is a versatile protein kinase that regulates many cellular functions. Growing evidence suggests that extracellular signal-regulated protein kinase 1/2 (ERK1/2) plays a crucial role in promoting cell death in a variety of neuronal systems, including neurodegenerative diseases. It is believed that the magnitude and the duration of ERK1/2 activity determine its cellular function. Activation of ERK1/2 are implicated in the pathophysiology of spinal cord injury (SCI). ERK2 signaling is a novel target associated with the deleterious consequences of spinal injury. ERK-2, also known as Mitogen-activated protein kinase 1 (MAPK1), is a member of the protein kinase superfamily and MAP kinase subfamily. MKP-3 is a dual specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases. Upon activation, MAPK1 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. MAPK1 is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. MAPK1 acts as a transcriptional repressor which represses the expression of interferon gamma-induced genes. Transcriptional activity is independent of kinase activity. The nuclear-cytoplasmic distribution of ERK2 is regulated in response to various stimuli and changes in cell context. Furthermore, the nuclear flux of ERK2 occurs by several energy- and carrier-dependent and -independent mechanisms. ERK2 has been shown to translocate into and out of the nucleus by facilitated diffusion through the nuclear pore, interacting directly with proteins within the nuclear pore complex, as well as by karyopherin-mediated transport. ERK2 interacts with the PDE4 catalytic unit by binding to a KIM (kinase interaction motif) docking site located on an exposed beta-hairpin loop and an FQF (Phe-Gln-Phe) specificity site located on an exposed alpha-helix. These flank a site that allows phosphorylation by ERK, the functional outcome of which is orchestrated by the N-terminal UCR1/2 (upstream conserved region 1 and 2) modules.


text:
Including and solving non-Newtonian correlations
Can someone tell me how to insert the non-Newtonian correlations (of a fluid) in the Navier-stokes equations and solve them. I mean discretize the equations and do the rest to solve the set of linear equations in Fortran, C++, or Matlab.
Re: Including and solving non-Newtonian correlatio
See for example
Finite Element Analysis of Non-Newtonian Flow Huang, Li, Usmani Springer
Numerical Simulation of Non-Newtonian Flow Crochet Elsevier
|All times are GMT -4. The time now is 01:58.


text:
Life Flight Network orders two AW119Kx
Leonardo-Finmeccanica has announced that emergency medical services (EMS) operator Life Flight Network has ordered two additional AW119Kx light single-engine helicopters for its fleet.
Life Flight Network operates across the Pacific Northwest and Intermountain West US. The order will bring the company's AW119Kx fleet to a total of 21 units by the end of 2016. The company also operates a twin-engine AW109 Power helicopter.
Leonardo Helicopters and Life Flight Network has also recently celebrated a 20,000 flight hours milestone by the operator's AW119Kx EMS fleet.
Email this to a friend.


text:
For sale by Maxwell Fidelis Okwelum - VERIFIED SELLER VERIFIED26 Jan 7:07 amLagos Mainland, Lagos
Toyota supplies only the best quality vehicles and this car is yet another example from their impressive fleet. This Toyota comes with a Automatic transmission system as well as other great features.
Stay safe!
Watch out for:


text:
Vogue 5990; ca. 1963; Car Coat and Pants. Coat in seven-eighths or short length with shoulder yoke has buttoned-through or toggle closing. Shaped collar or attached hood. Vertical welt pocket. Long or above wrist length sleeves.
Featured in Vogue Pattern Book October/November 1963, and Vogue Pattern Book June/July 1964
Links to reviews/blog posts Edit
Sources/Vendors Edit
Gallery Edit
[insert your photos of this pattern made up]
Wishlist Edit
[insert your username, and make sure your preferences allow for people to contact you via e-mail. Do not list your email address here!]


text:
White House press secretary Jay Carney said war games the U.S. and Poland began Tuesday are a show of united NATO force against the Russian incursion into Ukraine's Crimean peninsula.
Even though the U.S. says joint air drills and naval exercises in the Black Sea were scheduled before the crisis in Crimea, Carney said they send a message to Russia that the U.S. and its allies oppose its actions.
"This is part of our effort to work with our international partners and allies and to speak in a clear voice together that the actions taken by Russia to intervene militarily in contravention of international law and in violation of Ukraine's territorial integrity is something that we all oppose," Carney told reporters at Tuesday's press briefing.
Carney also said the U.S. is still waiting to see if the Russians are willing to resolve the crisis diplomatically with Secretary of State John Kerry in regular contact with his counterpart, Russian Foreign Minister Sergey Lavrov.
"They spoke again today and those conversations will continue," Carney said.
U.S. officials continued to underscore that there is still an escape route for Russia to avoid the full impact of Western sanctions and stressed that Washington understands that Moscow has and maintains interests in Ukraine, specifically their naval base in Crimea, as well as the protection of ethnic Russians.
If Russia continues to ignore diplomatic entreaties, Carney said there will be costs.
"We have a flexible tool in the executive order that the president signed that will allow us to calibrate the cost depending on the decisions that Russia makes, depending on whether or not Russia decides to avail itself of an opportunity here to pursue its concerns in Ukraine in a peaceful, diplomatic way," he said.


text:
Well.. i don't mind being 23.. i mean.. age is just a number. But what i do mind is, what have i achieved up to this day in my life? Some people has even own a company at this age, being this CEO of a company at this age, married at this age (nah..put that aside, i'm not ready for that yet)..
anyways.. rezeki orang ada di mana-mana. maybe it's not my time yet. insya-Allah la, mine, soon to come.. so what? you get to be 23 once in your life time..so tak kesah la..
Abah left for Abu Dhabi today..and Nurin had found a new interest, the TV. Constantly switching it on and off. And following me around. My shadow. Aishh si kenit ni. I neglected her a bit today, sebab penat and confused pasal interview venue this friday. Kesian die..Will make it up to her later la... her Auntie Mimie is around, so she'll be orait la.
Celebration? A small one with the family. Without Abah. But it's okay. Abah handed me his gift before he left. =) .. hmmm..
I wished for something good to happen. Amin
So my birthday wish list? (it's too late to write it here, but this list is for someone who asked me earlier what would i want for my birthday. ok, this list is for YOU)
- A nokia N93,
- A Coach handbag,
- PCD's hoodie,
- Nine West's shoes,
- Perlini silver's stainless steel ring, and
- YOU, who asked me for this list.


text:
Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
Plant genomics
Aphids are among the most destructive pests in temperate climates, causing significant damage on several crops including tomato. We carried out a transcriptomic and proteomic study to get insights into the molecular mechanisms and dynamics of the tomato response to the
The time course analysis of aphid infestation indicated a complex, dynamic pattern of gene expression. Several biological functions were affected and genes related to the stress and defence response were the most represented. The Gene Ontology categories of the differentially expressed genes (899) and identified proteins (57) indicated that the tomato response is characterized by an increased oxidative stress accompanied by the production of proteins involved in the detoxification of oxygen radicals. Aphids elicit a defense reaction based on the cross-communication of different hormone-related signaling pathways such as those related to the salicylic acid (SA), jasmonic acid (JA), ethylene and brassinosteroids. Among them, the SA-signaling pathway and stress-responsive SA-dependent genes play a dominant role. Furthermore, tomato response is characterized by a reduced accumulation of photosynthetic proteins and a modification of the expression of various cell wall related genes.
Our work allowed a more comprehensive understanding of the signaling events and the defense dynamics of the tomato response to aphids in a compatible interaction and, based on experimental data, a model of the tomato–aphid molecular interaction was proposed. Considering the rapid advancement of tomato genomics, this information will be important for the development of new protection strategies.
The investigation of plant defense mechanism offers interesting information about genes suitable to control agricultural pests
Aphids (Hemiptera: Aphididae) are the largest group of phloem-feeders and among the most destructive insect pests of cultivated plants in temperate regions
The signs and symptoms of aphid attack can be diverse, and vary according to the plant species (and the tissue attacked), to the aphid species and biotype, and their combination
Towards this aim, we studied at the transcriptional and proteomic level the defense response of tomato plants (
Expression profiling of tomato genes responsive to
To profile the variation in gene expression in tomato following the establishment of a compatible interaction with the
Figure 1
Hierarchical cluster of all differentially expressed probes
Hierarchical cluster of all differentially expressed probes. Distances were calculated using the Pearson similarity and agglomeration was performed according to the Ward's minimum variance algorithm. The heat-map diagram shows the relative expression level at the three time points (24, 48 and 96 hours post infection). Gradation from red to green represents strong up-regulation to strong down-regulation on a log scale. In each time point, each colored column represents a single biological replicate.
Additional file 1: Table S1.
Primers used for the expression study and their main features. Table S2: Differentially expressed genes 24 h after infestation with
Click here for file
Figure 2
Venn diagram showing the number of overlapping and non overlapping differentially expressed tomato genes in the comparisons between the three sampling times
Venn diagram showing the number of overlapping and non overlapping differentially expressed tomato genes in the comparisons between the three sampling times.
Dynamics of the tomato response to aphids
To underline the biological objective to which the differentially expressed genes contribute, we used the Blast2GO tool to provide Gene Ontology (GO) terms association. The differentially expressed genes were distributed in eleven categories, namely "cell maintenance", "transcription", "cell wall modification", "stress and defense response", "signal transduction", "photosynthesis", "primary metabolism", "secondary metabolism", "protein metabolism", "transport" and "unknown function".
The investigation of the tomato transcriptome following aphid attack highlighted the activation of a wide and complex response, as the transcriptional reconfiguration involved a broad range of biological processes in a different way. Since the absolute number of differentially expressed genes was different at the three time-points, Figure
Figure 3
Functional overview of the differentially expressed genes.
Functional overview of the differentially expressed genes. Functional analysis of the up-regulated (A) and down-regulated (B) tomato genes following
The classification of differentially expressed genes according to GO molecular function terms, indicated that "hydrolase activity" was the most frequent in the first (24 h) and last time (96 h) of infestation (Figure
Figure 4
Molecular functions of the differentially expressed gene.
Molecular functions of the differentially expressed gene. Distribution of molecular function terms for the up-regulated (A) and down-regulated (B) tomato genes following
Overall, both the number of genes and the categories of the differentially expressed genes indicated that transcriptional re-programming is a key process of a tomato defense towards
Tomato transcriptional response to aphids
The aphid response genes were grouped according to their functions. For sake of simplicity, genes that participate in more than one biological processes are presented only once considering their prevalent role.
Stress and defense response
This functional category comprised genes related to defense against abiotic or biotic stress, including those involved in the response to oxidative stress. Throughout the three time points, a total of 97 genes were differentially expressed (Additional file
Genes affected by aphid infestation were also related to the SA- (23 genes) and the JA- (8 genes) dependent pathways. Among the genes associated with the SA, there were chitinases, Pathogenesis Related (PR) proteins, and Nimin2c. The latter showed a transient boost, with the highest level of expression at 48 h (39,45 signal log ratio) and a reduced expression at 96 h of infestation (7,52). Nimin genes belong to small families linked to the SAR response, essentially through the interaction with NPR1
The gene expression analysis indicated that the transcriptional response to the accumulation and subsequent detoxification of ROS is taking place mainly at 48 h following infestation. Furthermore, aphid attack activates responses most similar to salicylate-mediated gene induction, although the expression of some jasmonate-related genes is also increased.
Signaling related genes
The signaling response in plants requires a variety of messengers and thus, seventy-six genes related to signal transduction were differentially expressed in infested tomato. These included among others, the LRR receptor-like serine/threonine-protein kinase, Serine/threonine-protein kinase receptor, S-locus receptor kinase and TIR-NBS-LRR resistance protein (Additional file
Overall, the transcriptional data indicated that tomato response to
Transcription related genes
Seventy-two differentially expressed genes were annotated as involved in the regulation of transcription (Additional file
Cell wall modification related genes
The data indicated that aphid infestation in tomato elicits a cell wall remodeling that could play a role in aphid resistance, further deterring insect probing and feeding on the unattacked host tissue.
Photosynthesis and primary metabolism related genes
The other fundamental biological processes in which the differentially expressed genes have been classified were photosynthesis (26 genes) and primary metabolism (77) (Additional file
Our data indicated that before the appearance of signs of chlorosis, the progression of aphid attack mostly represses photosynthesis-related genes in leaf as well as those involved in primary metabolism.
Cell maintenance, transport and secondary metabolism related genes
Seventy-three genes involved in cell maintenance showed differential expression following aphid infestation (Additional file
Array validation by qRT-PCR of defense-related genes
To validate the microarray results, the expression of nine differentially expressed genes was analyzed by Real Time-PCR. We selected genes annotated in different biological processes (biotic defense response, signaling transduction, phytohormone signaling and transcriptional regulation). The Additional file
Additional file 2: Figure S1.
Microarray validation and concordance with Real Time results (A) Relative gene expression analysis 24 h following aphid infestation. The graph displays the relative quantity (RQ) of each target gene in infested plants relative to the calibrator control plants. Asterisks indicate that the 2-∆Ct values were significantly different from the calibrator (p < 0.01; Student's t-test). (B) The graph displays the concordance between microarray fold change and Real Time RQ values on a linear scale. Figure S2: Microarray validation and concordance with Real Time results (C) Relative gene expression analysis 48 h following aphid infestation. The graph displays the relative quantity (RQ) of each target gene in infested plants relative to the calibrator control plants. Asterisks indicate that the 2-∆Ct values were significantly different from the calibrator (p < 0.01; Student's t-test). (D) The graph displays the concordance between microarray fold change and Real Time RQ values on a linear scale. Figure S3: Microarray validation and concordance with Real Time results (E) Relative gene expression analysis 96 h following aphid infestation. The graph displays the relative quantity (RQ) of each target gene in infested plants relative to the calibrator control plants. Asterisks indicate that the 2-∆Ct values were significantly different from the calibrator (p < 0.01; Student's t-test). (F) The graph displays the concordance between microarray fold change and Real Time RQ values on a linear scale.
Click here for file
Proteomic analysis of
We also carried out a proteomic analysis of tomato leaves after
Figure 5
2-DE proteomic map of tomato leaves from non-infested tomato plants
2-DE proteomic map of tomato leaves from non-infested tomato plants. Protein extracts were analyzed in first dimension (pH 4–7 linear IPG, 18 cm); second dimension was performed on a vertical slab (12%T) gel. Protein detection was achieved by using colloidal Coomassie staining. Numbering refers to differentially-represented protein spots in the
Figure 6
Assignment of Gene Ontology (GO) terms for differentially-expressed proteins after
Assignment of Gene Ontology (GO) terms for differentially-expressed proteins after
Protein-mRNA comparative analysis
A correlation of transcriptomic and proteomic data was performed considering the genes that give rise to the differentially expressed transcripts or proteins. A first comparison, focusing on the functional categories in which the different protein species and transcripts were ranked, indicated a correspondence between the identified GO categories. For both "omics" studies, the functional category "defense and stress response" was the most represented. The match between genes and proteins was very limited, with only 3 mRNA-protein identities (Table
Our study generated extensive data on the expression of a large number of tomato genes and proteins during critical periods of infection with aphids and it provides the first insight into the dynamics of the response to
Even if aphids are able to establish a long-lasting intimate interaction with plant cells, the tomato reaction was clearly variable during the course of the infestation. The number of differentially expressed genes considerably increased from 24 h to 48 h, reaching also the higher magnitude of expression, and declined at 96 h. This trend may be explained considering that a multifaceted defense response is costly for the plant, which should try to balance defense induction and impact on fitness traits following probing and establishment of a feeding site. Furthermore, in a compatible plant-insect interaction, it is likely that the reduced magnitude of the response at the last stage is the result of plant reprogramming the injured leaf to handle the progression of a successful infestation, along with an active aphid deception of tomato defense
For a more integrative view of the tomato-aphid interaction, the transcript profiling was complemented by a proteomic study. We confidently identified 57 proteins that were differentially expressed after insect attack. These proteins belong to a set of biological processes that covered the functional groups of the transcriptional analysis. For instance, response to stress and alteration in photosynthesis were the most abundant categories. The little overlap between trancscriptomic and proteomic data is in accordance to other combined studies in plants
Based on the transcriptomic and the proteomic data, we propose a model to depict the main components involved in aphid response in tomato (Figure
Figure 7
A model summarizing the signaling events and molecular response in tomato following aphid attack
A model summarizing the signaling events and molecular response in tomato following aphid attack. Selected up-regulated genes are in red color, while down-regulated genes are in green. Black lines with arrows indicate activation of enzymatic activities, induction or accumulation of compounds. Grey dashed lines represent indirect positive interactions. Black blunted lines represent inhibitory associations. See text for details.
Tomato should perceive aphids essentially through cell membrane receptors and subsequently, the signal transduction exploits various cellular messengers, principally ROS, calcium and stress hormones
The tomato transcriptional reconfiguration relies in a number of TFs, and a pivotal role is played by WRKY proteins. As some of these proteins mediate the cross-talk between JA-mediated and SA-mediated signals during plant defense, it is reasonable to speculate that the WRKY genes that are continuously overexpressed during the aphid infestation, are involved in the antagonist interaction between SA and JA, as reported for the WRKY70 in
Although the number of differentially expressed genes and proteins that can directly affect aphid performance was low in percentage, the tomato response includes changes in cell structure and plant metabolism that can successfully limit aphid infestation, as also observed in other interactions
Transcriptomic and proteomic data showed a consistent reduction in the category 'photosynthesis'. The transcriptional downregulation of photosynthetic-related genes appears to be a kind of universal adaptive response of plants to biotic stress, which may be compensated by a slower turnover of many photosynthetic proteins
Despite the relevant economic impact, not much is known about the molecular recognition and response of tomato plants, and more generally of Solanaceae crops, to aphids. Our study provided a detailed overview of the transcriptomic and proteomic responses of tomato to aphids and led to a more comprehensive understanding of the signaling events and the defense dynamics. Different molecular cues, including those associated to tissue damage and elicitor recognition, lead to a complex, dynamic pattern of expression, in which distinct groups of similarly behaving transcripts were observed. Early events of the response support a gene-for-gene interaction and sensing of a wound-induced damage. The Gene Ontology categories of the identified genes and proteins indicated that the local response is characterized by an increased oxidative stress accompanied by the production of proteins involved in the detoxification of oxygen radicals. Aphids elicit a defense reaction that involves different hormones, with the SA-signaling pathway and stress-responsive SA-dependent genes playing a dominant role. The wound-inducible JA pathway was not strikingly affected, although some JA-dependent genes coding for anti-nutritive proteins were up- or down-regulated. Finally, tomato response is characterized by a reduced investment in photosynthetic proteins and a modification of the expression of various cell wall-related genes.
The identification of genes involved in aphid defense, provides a reference line for the screening of tomato genomic resources, eventually impacting other economically important Solanaceae crops. In the future, targeted functional studies should follow to elucidate the role of the here presented genes in the tomato defense, essential for the development of rational strategies to enhance a durable broad-spectrum resistance to aphids in tomato.
Biological material
Four weeks old tomato plants (
Microarray analysis
RNA purification, labeling and oligonucleotide microarray hybridization
Approximately 200 mg of leaf tissue were ground to fine powder in liquid nitrogen and homogenized in Qiazol solution (Qiagen). RNA was isolated using the RNeasy Plus Mini kit (Qiagen), according to manufacturer's protocol. RNA samples were analyzed quantitatively and qualitatively with a NanoDrop ND-1000 UV–vis Spectrophotometer (NanoDrop Technologies) and by a Bioanalyzer (Agilent Technologies), respectively. Only samples with an RNA Integrity Number (R.I.N.) >8 were used for RNA labeling. Total RNA was amplified in the presence of cyanine-3/cyanine-5 labeled CTP using the Agilent low RNA Input Fluorescent Linear Amplification kit (Agilent Technologies), according to manufacturer's instructions. After labeling, reactions were purified using the columns of the Qiagen's RNeasy kit. The quality of labeled targets was determined by calculating the amount of cRNA concentration (ng/μl), Cyanine 3 or cyanine 5 dye concentration (pmol/μl) and RNA absorbance ratio (260/280 nm) using a NanoDrop Spectrophotometer. The specific activity was calculated using the formula: (Concentration of Cy3 or Cy5) / (Concentration of cRNA) * 1000 = pmol Cy3 or Cy5 per μg cRNA. Only samples with a specific activity ≥8 were used for hybridization. Equal amounts of cRNAs from control and from infested plants were mixed together and hybridized to the microarray in a hybridization oven at 65°C for 17 hours with rotation speed set at 10 rpm.
Gene expression profiling was performed using the Tomato Gene Expression array (4x44k) (Agilent Technologies). This array contains over 44000 probes, representing more than 21,000 tomato transcripts. After hybridization, slides were washed with Gene Expression Wash buffer 1 for 1 minute at room temperature, and Gene Expression Wash buffer 2 for 1 minute at 37°C. Finally, arrays were treated with the Stabilization and Drying Solution (Agilent Technologies) for 30 seconds at room temperature. Immediately after washing, slides were scanned with the Agilent Dual Laser Microarray Scanner and image data were read out and processed by the Feature Extraction v. 10 software (Agilent Technologies).
Data analysis
The GeneSpring® 10 (Agilent Technologies) software was used to process the microarray data and to associate sample information. Statistical analysis was performed using background-corrected mean signal intensities from each dye channel. Microarray data were normalized using intensity-dependent global normalization (LOWESS). Statistical testing of differential expression was performed using the Benjamini-Hochberg False Discovery Rate method with a cut off (
Microarray validation by real-time quantitative PCR analyses
Two μg total RNA were reverse transcribed by using a SuperScript II Reverse Transcriptase (Invitrogen), following the manufacturer's protocols. To control cDNA synthesis and PCR efficiency, the amplification of the constitutive gene coding for the Elongation Factor 1-a (EF1-a) was carried out according to already published procedures
Proteomic analysis
Proteins were isolated from leaves and resolved and scanned in 2D gels as described
Protein digestion and MS analysis
Spots from 2-DE were manually excised, triturated,
Protein identification
The MASCOT software package version 2.2.06 (Matrix Science, UK) was used to identify proteins present in gel spots from a tomato Unigene database (
2-DE: Two-dimensional gel electrophoresis; ABA: Abscissic acid; CDPK: Calcium dependent protein kinase; Et: Ethylene; GO: Gene ontology; JA: Jasmonic acid; LRR: Leucin rich repeat; MALDI: Matrix-assisted laser desorption/ionization; MS: Mass spectrometry; NADPH: Reduced nicotinamide adenine dinucleotide phosphate; NBS: Nucleotide binding site; PPO: Polyphenol oxidase; PPR: Pentatricopeptide repeat-containing; PR: Pathogenesis related; RLK: Receptor like kinase; ROS: Reactive oxygen specie(s); SA: Salicilic acid; SAR: Systemic acquired resistance; TOF: Time of flight
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
VC performed the microarray and the gene expression analysis, analyzed the data and drafted the paper, MC contributed to the microarray experiments and the functional annotation, MR performed the 2D-Electrophoresis, CD carried out the aphid infestation, CDA and GR executed the analysis of the proteomic spots, RM provided assistance for the microarray experiments, AS analyzed the proteomic data and contributed to the writing of the manuscript, FP reviewed the manuscript, RR participated in the study design and reviewed the manuscript, GC designed the study, analyzed the data and wrote the manuscript. All authors read and approved the manuscript.
This research activity was supported by the F.A.R.O. project, Università degli Studi di Napoli e Compagnia San Paolo. The transcriptomic study was partially supported by the P.O.N. Ricerca e Competitività (2007–2013) Genopompro project. The proteomic study was partially supported by grants from the Italian Ministry of Economy and Finance (Progetto Innovazione e Sviluppo del Mezzogiorno - Conoscenze Integrate per Sostenibilità ed Innovazione del Made in Italy Agroalimentare" - Legge n. 191/2009). We thank Adele Cataldo for her technical support throughout the project.
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Justin Bieber - The Feeling (PURPOSE : The Movement) ft. Halsey MP3
'Purpose' Available Everywhere Now! iTunes: http://smarturl.it/PurposeDlx?IQid=VEVO1113 Stream & Add To Your Spotify Playlist: http://smarturl.it/sPurpose?
The Feeling
Justin BieberYou are to me A part of me just like anatomy You're pulling me You're pulling me in like you're gravity I'm notorious for thinking you're full of beautiful Instead of hollow Sugar on your lips, it's hard to kill Jagged like a pill, s[...]
JAY-Z - Dirt Off Your Shoulder MP3
Music video by Jay-Z performing Dirt Off Your Shoulder. (C) 2003 Roc-A-Fella Records, LLC.
A Song for if you're feeling sad. MP3
CutCakeNotWrists. So I was looking through my emails and I had an email from a young girl saying she was feeling really low and depressed. And I thought this ...
Five - Got The Feelin' MP3
Five's official music video for 'Got The Feelin''. Click to listen to Five on Spotify: http://smarturl.it/FiveSpotify?IQid=FiveGTF As featured on Five: Greatest Hits.
Got The Feelin'
FiveNa na na na na nah Na na na na na nah(x4) Here we go again with the beats We got you heads bumping, now you're jumping from your seats If this is what you're wanting over there Throw your hands up in th[...]
Happy Video for if You're Feeling Sad MP3
The song is Enchanted by Taylor Swift! Just wanna remind all the girls and boys in pain, that it won't last forever if YOU DON'T LET IT! Feel free to comment ...
Belle & Sebastian - If You're Feeling Sinister Lyrics MP3
I don't own this song.
My Depression Story: Where I've Been & What I'm Feeling MP3
I can't believe I'm uploading this. Um. So. These are some things I just felt I needed to say. It's okay not to be okay. I want you to know that. That you're not alone ...
Gregory Isaacs - If You Feeling Hot, I Will Cool You 1977 MP3
Gregory Isaacs - If You Feeling Hot, I Will Cool You - Willow Tree - 1977 - Produced by Alvin Ranglin - Engineer Ernest Hoo Kom & Maxie - Channel One Studio ...
What To Do If You're Feeling Unwell on the Tube MP3
If you feel ill on the train, you'll receive help more quickly if you get off at the next station where members of station staff can help you. If you're on the train and ...
Passenger - Let Her Go [Official Video] MP3
The official video for 'Let Her Go', as featured in the Budweiser Superbowl 2014 commercial. Directed and Produced by Dave Jansen. Amazing work Dave ...
Justin Bieber - Love Yourself (PURPOSE : The Movement) MP3
'Purpose' Available Everywhere Now! iTunes: http://smarturl.it/PurposeDlx?IQid=VEVO1113 Stream & Add To Your Spotify Playlist: http://smarturl.it/sPurpose?
Love Yourself
Justin BieberFor all the times that you rain on my parade And all the clubs you get in using my name You think you broke my heart, oh girl for goodness sake You think I'm crying, oh my oh, well I ain't And I didn't wanna write a song cause I didn't want anyone thinking I still care I don't but, you still hit my p[...]
If You're Only 20-Something, Stop Saying You're Old MP3
Your grandpa thinks he's old because he fought in World War II. You think you're old because you saw Mean Girls in theaters and you're an idiot. See more ...


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Tea Party Claims Victory in Jim Greer Resignation; Already Complaining About his Replacement
Tea Baggers have long been complaining about soon-to-be-former RPOF Chair Jim Greer, but the Republican establishment didn't get serious about showing Greer the door until a bunch of deep pocketed donors flaunted an open letter that it was time for a change in leadership.
That hasn't stopped the movement from claiming victory though.
"A founder of the 'tea party' movement said Wednesday he had a warning for Republican state leaders: Back conservative candidates or else other states will suffer the same backlash that toppled Florida's Republican Party chairman this week," reports the conservative-leaning Washington Times.
"If they continue to do things like they did in Florida, it's not going to be good for them," Dale Robertson told the moonie sheet.
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We don't doubt that the right-wing pressure made the decision easier to send Greer down the road, but let's be clear, it was his handling of money and public complaints by donors that broke the camel's back.
How much of a victory is it, though, when some grass roots activists are already complaining about Greer's expected replacement Sen. John Thrasher. The man's a tad bit more conservative, has the backing of Jeb Bush, and attended a Marco Rubio fundraiser, but he's not expected to do much to change the dynamic in the Rubio v Crist war. Plus he's a little bi-curious, bipartisan curious that is: he wrote a check to Democrat Alex Sink's 2006 campaign for CFO.
One state committee woman, Sharon Day of Broward, is saying
that the grass roots were disrespected by Thrasher's anointment. Of
course, she's making a last minute run for the gig herself.
Marco Rubio, tea party poster boy, isn't endorsing her, but he's not endorsing Thrasher either. Make of that what you will.
Meanwhile, pundit Chris Ingram says Thrasher is a "big-business corporate-money," who's no different than many democrats.
grassroots of the Republican Party doesn't know a whole lot about John
Thrasher. This however is good if you're John Thrasher. In an attempt
to make him look like the next crown-prince, the party elders and big
money men have unified to rally support for Thrasher before the serfs
learn too much about him," writes Ingram.
There's also concern that Thrasher is a sitting state Senator whose focus would be taken away from the job when the body is in session.
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I am American and I wish I had a president that cared enough to clean up the US this way. Duterte truly cares about his people
- Blogger Comment
4 comments:
the foreigners acknowledged the good deeds and intentions of the president..but some of us, are like crabs,, trying to pin him down, praying for his downfall..esp. the yellowtards army..if something happens to the philippines.. the first one to leave the country is this yellow army because they have the money, connection and they can easily get visas while those who believe in them will be left behind and sufferedReplyDelete
- AnonymousAugust 21, 2016 at 7:17 PM
This is a waking word for those who still dont understand our president..thank u for having a widen mind and ipen heart ti understand our president love to our country..ReplyDelete
Pres. Duterte should be very careful with the Vatican, CIA and the Mainstream Media that was owned and controlled by the Network of Global Corporate Control (identified by by Vitali, Glattfelder, and Battiston of ETH Zurich http://arxiv.org/PS_cache/arxiv/pdf/1107/1107.5728v2.pdf )
Read the teleprompter so that you may know how Vatican, CIA and Switzerland are interconnected. This is the Teleprompter of Karen Hudes DCTV Show.


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Distances are calculated in a straight line and may not reflect actual travel distance.
It is important to note that this mortgage calculator is for guidance purposes only. Mortgage lenders have their own affordability criteria therefore it is important you speak to one of our Mortgage Advisers for further information and a more accurate indication of what products may be available to you and what your monthly mortgage payments are likely to be.
Situated within a private and tucked away position in this popular village to the North of Banbury, a versatile family home with countryside views. The property offers light and open plan accommodation offering a split level sitting and dining room with a central fireplace and stove. The kitchen and breakfast room offers a range of eye and base level units with a Belfast sink and granite worksurfaces, a Leisure 6 ring, double electric oven and grill (available by separate negotiation). A utility room leads from the rear entrance hall along with the boiler room. In addition to the main accommodation there is a further garden room with a wood burning stove and views to the garden. From the sitting room there is further access to an additional kitchen/breakfast and living room with a wood burning stove to one corner. Stairs lead up to a bedroom and a bathroom, this area would make an ideal annexe or could be considered as part of the main accommodation. The master bedroom benefits from an ensuite bathroom and there are three further bedrooms, a further ensuite and the family bathroom.
Upper Boddington is located north of Banbury. Within the village there is a public house, a bi-weekly post office facility, village hall, school, public playing field and church. More facilities can be found in Banbury with its mainline railway station into Birmingham or London (Marylebone approx 51 minutes). Daventry also offers a mainline railway station into London (Euston). Access for the M40 south can be found at Banbury (J11) and at Gaydon (J12) for those heading north to the M42. There is also access to Coventry via the A423 and the M1.
The property is approached via gated access with driveway parking for a number of vehicles and access to the garage / store. The property is centrally positioned within the plot with the gardens extending to lawn and shingle area with various seating areas surrounding. A decking area is situated near to a pond with a walkway joining the garden and giving views across the local countryside as well as a summerhouse. A further decking area can be accessed via the garden room making an ideal entertaining space for the family.
Additional Information
Oil Fired Central Heating. South Northamptonshire Council, Band E.
5 Bedrooms
Two Ensuite Facilities
Open Plan Sitting & Dining Room
Kitchen/Breakfast Room
Utilty Room
Garden Room
Additional Kitchen
Lounge & Ensuite Facility
Family Bathroom
Garage and Extensive Parking
Contact branch for relevant Energy Performance Certificate
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wecare program download free id:EWWL full version
== Search results for wecare program ==
Searched file: wecare program
*Filename: wecare program.zip
*Year: 2015
*Total Downloads: 153451
*Uploaded: 03/01/2015 14:55:22
*Type: WinZip
*Supported OS: All
== Searches related to wecare program: ==
03/01/2015 14:55:22
The We Care Program, since 1970, has been sharing the transforming love of God with troubled men and women in prison and beyond.WeCare: The Wellness, Comprehensive Assessment Rehabilitation and Employment (WeCARE) program is a unique initiative that addresses the needs of cash …We Care home care services ensure that your loved ones are comfortable at home for as long as possible. We provide top quality home health care services.We Care Spa specializes in fasting and colon cleanse detoxification, lifestyle detox education, and anti-aging rejuvenation. 25 years, Palm Springs CAOnline courses for Long Term Care Facilities, including Medication Aide Update, Activities Director, Social Service Designee, Medical Terminology, Restorative Aide ...Enjoy great promotions and deals from Jack Astor's. Buy a Jack Astor's Gift Card.A donation to We Care Solar in February can support our student education program, called We Share Solar. We Share Solar teaches American youth to build Solar ...We Care Counseling Center, counseling, services, individual services, programsTo Show We Care is an all volunteer organization that hosts performing arts events so that people with life threatening illnesses can attend for freeSearch Our Programs Search Program Directory: Find a Program to fit your needs: Select State:
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January 26, 2010
Lecturer in Fine Arts Matt Dubroff of the Hampden-Sydney Theatre Department toured with Theatre Nohgaku over the Winter Break. (Dubroff is pictured at right in production at Hampden-Sydney in 2006.)
The Oshima Noh Theatre of Hiroshima Prefecture, Japan, and Theatre Nohgaku, based in Tokyo and New York, collaborated for the first time in a joint production of classical and contemporary Noh Theatre
from December 2 to 10, 2009, in London, Dublin, Oxford, and Paris. This was a rare and unique international production featuring an extract from the 15th century classical warrior play Kiyotsune and the world premiere of Pagoda, a new English-language Noh play written by Jannette Cheong with music and direction by Richard Emmert. This was the first time for a strictly English-language Noh play to be written by a British playwright as a fully realized Noh performance. The production also included "Getting to Noh": a program of public workshops, lectures and educational activities to introduce the history, structure, dance, music, costumes, and masks of Noh.
This international project brought together many skills and talents from Europe, Japan, and the USA. Twenty-six people participated in the tour including the writer, the mask-maker, and the artist who created the Pine Tree scroll paintings.
The Oshima Noh Theatre is part of the Kita school, one of the five main-actor Noh schools. It features members of the Oshima family, four of whom participated in the tour - both production and performance.
Theatre Nohgaku is an international company comprised of Japan and North American based members. Its mission is to create and present English-language plays in traditional Noh style complete with hayashi musicians, masks, costumes, and stage sets. Led by Artistic Director, Richard Emmert, it serves as a unique cultural and artistic intermediary between Japan and the English-speaking world.
ballet.com magazine
The Stage


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Re: Louis John Mohr & Catherine Pruett-Balto.MD
In reply to:
Louis John Mohr & Catherine Pruett-Balto.MD
Margaret Keigler 10/19/01
I don't know if there is any relation, but yours is the first Mohr in Baltimore that I've come across. In my family there was a Mary Mohr who married Heinrich O. Stintz in the late 1800's. (He was from Hamburg, Germany.) They lived in the Patterson Park area & then on Maple Avenue. Also in Baltimore in the late 1800's/early 1900's was a Kate Mohr who married John Lassohn, Jr.Sorry I don't know if related.


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The Advoc8te scored a ticket to tonight's annual fundraising event, AfterDark@THEARC. This gala recognizes the many people and organizations that make THEARC special and raises money to continue THEARC's efforts to make education, arts, music and dance programs accessible to the District's residents, particularly those in Ward 8.
Follow my tweets at #THEARCgala for pics and updates on tonight's event.
Tweets about "#THEARCgala"


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I spent a day skiing this past week at Red Lodge in Montana. It was pretty much my first time skiing, since I don't really count the time back in 2000 when a close friend convinced me to spend a few hours at Snowbowl just outside my hometown in Elko, NV. Snowbowl is not so much a mountain, as much as a medium-sized hill. What I remember most vividly about my first experience skiing at Snowbowl ten years ago is that I walked away (barely) with a couple of twisted knees – injuries that continued to mildly plague me for the next couple years.
But Red Lodge isn't kidding around, and I was pretty freaked out by the size of this mountain. I was convinced I was going to ride home in an ambulance after snapping both legs backwards or something. But do not fear, dear readers, this is a story of victory and triumph, which I will relate to you in exquisite detail:
Our adventure started off with an hour-long wait at the ski rental shop. Waiting was frustrating, but I was secretly happy to have an extra few minutes with my knees while they still worked. Skis in hand, we finally made our way halfway up the mountain for the first time on the ski lift.
We chose an easy way down for our first run – a green circle run, which the locals tell me "are for chumps". I did pretty well, although I fell a few times. And fell. And fell. And fell.
But then I started falling less and less, and most of my falls were semi-controlled and not too big a deal. And after a few times on the chump runs, I was feeling ready to take on a harder challenge – the blue square runs (which the locals tell me are "also for chumps", but that's just because they're ass-holes).
I proclaimed, "I will do ALL of the runs!" and directed Mel to lead me higher up the mountain to areas that were visibly steeper and narrower. When we got off the lift at the top of the mountain, we chose an out of the way run called "Barriers", which proved to be an appropriate name for Satan's gift to skiers.
The first 100 feet of this run were fine. Smooth skiing, not to steep. But then it got steeper, and we figured out why it was named "Barriers." Moguls – massive snow warts that mar the face of an otherwise perfectly good ski run.
Then the terror began to set in. What was I doing on this hill? What am I doing with these giant popsicle sticks strapped to my feet? Who the hells idea was this? I was in over my head.
The next few hundred feet were accomplished about 20 feet at a time, with me falling flat on my face "to rest" between each segment. After about 20 minutes of this, I was finished with "Barriers". Emotionally, I mean – I was physically still towards the top of this bastard mountain with no other way down. I'd used up all my four-letter words, and cursed all the deities I could remember. I'd twisted my knees about a dozen times already.
I looked around for sharp sticks. "If I could jam one of these into my leg far enough, they'd have to send someone up to slide me down the mountain in a little sled," I thought. No such luck. Mel had noticed that I was "taking a break" and was watching me. I'd have a hard time explaining it to her if she watched me maim myself.
Cranky, tired, and frustrated after my 200th fall of the day, I swallowed my pride, took off my skis, and crawled on all-fours to the next run over – an easier run. Something more appropriate for a chump like myself. Confidence shattered, I slowly made my way down the mountain, falling several more times, and filling my pants with snow.
We quit for lunch, and as my muscles cooled, they became stiff and sore. I was in sorry shape – emotionally drained, and physically weak. But the day was only halfway done.
After lunch, we continued to ski, and I continued to fall. In fact, my worst performance yet came after lunch. Falling all over the place, and on mild hills that I shouldn't have had any problems with, things were looking bleak.
But like I said, dear reader, this is a story of victory and triumph. Not over the mountain, however, but over myself. In no sense did I "conquer the mountain" or "improve my technical skills through practice and patience". But I did continue to ski until dusk, picking myself up when I fell, and silently rejoicing at the sight of the "slow ski zone" banners at the bottom of each run. I am not good at skiing, but I finished the day without intentionally injuring myself so that I could spend the rest of the day in the warming hut sipping cocoa. I got back up every time I fell, and walked away at the end of the day, albeit with very sore and swollen knees. And that, dear reader, is a victory.


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April 22, 2010-Earth Day Statement of Rey Ramsey, President and CEO of TechNet
Earth Day Statement of Rey Ramsey, President and CEO of TechNet
Earth Day is both a day to celebrate the environment and consider how we can become better stewards. Technology it is now our best tool for promoting sustainable and clean living. Environmental awareness is a critical component not only to the technology industry¿s bottom line, but also to our collective economic and national security.
The voting public agrees and, according to a recent Zogby-463 survey*, nearly one-third of respondents think that investments in green energy have the best potential for creating high quality jobs while forty-five percent believe that technology has the best chance of reducing our dependence on foreign oil.
We call on our elected officials to join us in completing work on a national clean energy policy that will appropriately price carbon emissions and fund R&D efforts that stimulate advances in environmentally beneficial technologies.
*The Zogby-463 survey of 4,143 likely U.S. voters was conducted from March 12-15 with a margin of error of +/- 1.6 percentage points.
About TechNet:
TechNet is the national, bipartisan network of CEOs that promotes the growth of technology industries and the economy by building long-term relationships between technology leaders and policymakers and by advocating a targeted policy agenda. TechNet's members represent more than one million employees in the fields of information technology, biotechnology, e-commerce and finance. TechNet has offices in Washington, DC, Palo Alto, Sacramento, Seattle, Boston and Austin,Texas. Web address: www.technet.org. You can also follow us on Facebook and Twitter at @technetupdate.


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Shackelford's High Flying Coaster: See how high you can fly with Shackelford in this fast and furry roller coaster!
Rodrigo's Weight Lift Drops: Rodrigo will drop his weights along with your help as you shoot across the sky in this drop tower like ride.
Dorothy's High Wire Pendulums: Help Dorothy get over her fear of heights in this high to the sky pendulum ride!
Dirk's Extreme Flyers: Go extreme in this thrill seeking plane ride with Dirk!
Amazing Animal Treats: You will love the candies, chocolates, cotton candies, marsmallows and other sweet treats at this food vendor!
Shackelford's Amazing Pastries: Pies, Danish pastries, profiteroles, choux pastries and strudels are sold here to complete your day a high flying good time!
Rodrigo's Casa de Helado: Fresh homemade ice cream are served here with various flavors.
Dorothy's High Wire Refreshments: Cold drinks, fruit juices and smoothies are sold here at this vendor!
Dirk's Extreme Pizzeria and Pastas: A daredevil like Dirk himself just couldn't resist Italian food! This cool dude dachshund dog serves various types of pasta with different sauces and pizzas.
Larry's Amazing Animal Emporium: You can find many T shirts, plush toys, hats and more at Larry's amazing emporium!
Amazing Animal Gifts: More character plush toys are sold here in this amazing toy shop!
Stage shows:
Larry's Amazing Animals Live!: You can watch Larry and his amazing animals live as they perform their usual daring feats and stunts from their own TV show and Clifford's Really Big Movie! As usual, Larry will announce each act to visitors of the park.
Larry and his Amazing Animals appear as Meet and Greet characters


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Anyone who operates a Chevrolet knows that when it comes to repairing their vehicle only the highest quality replacement parts will do. Your Chevrolet dealership isn't the only place to locate the best replacement parts for your R1500 Suburban. It's not all the time that you realize that you have to buy a replacement Drum Brake Shoes to fix your vehicle. Make no mistake, you bought your R1500 Suburban because you understood you would be getting horsepower and good looks both impeccably combined into one vehicle.
The ability to slow down and make quick evasive maneuvers is crucial to keeping you and your passengers safe. However, it took a couple of decades of automobile production until drum brake shoes were invented. Drum brake shoes appeared on almost all vehicles from the 1920s to the 1970s, and your Chevrolet R1500 Suburban uses them inside the drums. Much like all brake systems, pressurized brake fluid is sent from the master cylinder to each wheel where the Chevrolet R1500 Suburban drum brake shoes create friction. You come to a stop when energy is absorbed by the brake shoes as they are pressed up against the drums.
Buying new Chevrolet parts from www.carpartsdiscount.com means you are paying the lowest prices for the highest quality original and aftermarket replacement R1500 Suburban parts on the internet. Bring your R1500 Suburban back to life with the highest quality repair parts from the experts at Car Parts Discount. At Car Parts Discount, we have live customer service members available with enough expertise to help you select the correct Chevrolet R1500 Suburban part for your needs. When it comes to Chevrolet R1500 Suburban Drum Brake Shoes parts, you can find them for 1991, 1990, 1989 models by clicking the appropriate year below.