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Help us name our conference mascot! What is the perfect name for our little visitor who is Reading Our World and Inspiring the Future? We'll pick the top 5 entries for a run-off vote on Facebook to determine the grand prize winner! This contest is open to all ages! The deadline is June 15, 2017, and there are prizes available! Click here to enter the contest!
The 51st annual conference will be held in Richmond, VA from March 8-10, 2018, at the Richmond Marriott and Convention Center. Please consider submitting a proposal for a conference session. The first call for proposals will be on June 15, 2017. A final call for proposals will be on August 25, 2017. Proposals can be submitted here via Google Forms.
All proposals will be reviewed beginning on August 26, 2017, and we do not anticipate extensions for this deadline. Presenters will be notified of proposal acceptances by November 30, 2017. Please direct any questions to Joan Rhodes, the 2018 conference chair, at firstname.lastname@example.com.
You may use the following link to obtain a PDF version of the proposal to assist with your planning:
http://bit.ly/2o6DKpV However, the Google Form must be used to submit your final proposal.
Thank you for joining us for our 50th Anniversary, we hope to see all of you again next year in Richmond!
ATTENDEES: To obtain your Certificate of Attendance you must complete the post-conference survey sent to your email. (If you did not receive it, please contact email@example.com or firstname.lastname@example.com and it will be re-sent to you.)
Primary: I Yam A Donkey by Cece Bell
Elementary: The Terrible Two by Mac Barnett and Jory John
Middle: Echo by Pam Muñoz Ryan
High School: I'll Give You The Sun by Jandy Nelson
Click here to access a bookmark that recognizes this year's VRC winners!
The 2017-2018 Virginia Reader's Choice book lists are now available!
Click here to access the Primary, Elementary, Middle School, and High School lists.
Our 2016-2017 Membership year has ended. Please access this page, beginning on August 1, 2017, to become a member for the 2017-2018 year.
The cost of joining VSRA and a local council is $30. The cost is only $20 for paraprofessionals, retirees, and full-time students. Can't decide which local council to join? Check out the list of local councils on the Membership page. Consider joining more than one council to increase your professional development opportunities and broaden your networking circle. There is also a statewide council for college reading educators. They are great councils to join in addition to your local council(s). Members may add a dual and tertiary council for $10 each. Click here for more information about the value of membership in VSRA.


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Welcome to Canada on your Fingertips, a website dedicated to serve as a one-stop Encyclopedia for Canada, be it the country's past or present, hosted by our partners MaxiShare. Right from the culture, demographics and historical splendor, to the various tourist attractions, governmental initiatives and welfare projects, there are so many wonderful things to explore here, in the online encyclopedia that goes by the name of Canada on your Fingertips.
Trivia: Did you know these interesting facts about Canada?
- Most of us are well aware of the fact that Canada happens to be the second largest country in the world as far as the total land and marine territories under control are concerned. In terms of simply calculating the total land area, the ranking is gauged as No.4. But the lesser known fact is that the length of the border that Canada shares with the United States, is the longest land border in the entire world!
- Contrary to popular belief, it was not the British who were the first explorers or colonizers to have set foot in Canada. Rather, it was the French who are accredited with the establishment of the first colony in Canada, all the way back in 1534!
- Did you know that the Head of the Federal Parliamentary Democracy of Canada is the Canadian President? Well, of course you do. But the lesser known but widely prolific fact is that Canada is also essentially a constitutional monarchy and it is the Queen of Great Britain Elizabeth II who is the current head.
- Not only does Canada feature in the top 10 list of the world's best countries in terms of Human Development Index, but also happens to be one of the most liberal countries in the realm of welcoming and accommodating refugees. Time and again, the Canadian ethos of liberalism and multi-cultural beauty have been hailed by world bodies. Apart from the most-measured metrics of per capita income and gross domestic product, this North American country ranks pretty high as far as social parameters such as education, civil liberty and freedom are concerned.
- The crux of Canada's affluence can primarily be accredited to two factors- the first that it has excellent reserves of natural resources such as forest cover, fossil fuels, marine resources etc. The second reason is that people from various communities across the world have migrated here, and not just added to the diversity of culture, but also the strengthening and vibrancy of the economy in various sectors.

subset: commoncrawl


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From just £149* a family of four can visit any 3 out of 10 fascinating Leicestershire attractions including Twycross Zoo, and enjoy overnight accommodation including breakfast at a choice of 4 star family friendly hotels including Jurys Inn Hinckley Island, Hilton Leicester and Sketchley Grange Hotel and Spa.
During Bank Holiday weekends there is a premium rate of £159 charged for this break for 2 adults and 2 children.
For more information and to book your Stay Play Explore short break, please visit the Stay Play Explore web site > http://www.stayplayexplore.co.uk/


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C-TECH gaming headset Kalypso (GHS-04R), black-red
Product description:
Headphones KALYPSO are new additions to the game series of C-TECH. In their development, the emphasis was on every detail. Earphones are made of high quality materials and when using them on the head thanks to the comfortable padding will not even after several hours of use almost know. After the sound will be satisfied even the most demanding users, since KALYPSO headphones are equipped with high-quality 40 mm converters that render crisp highs and deep bass. This will be appreciated by gamers, as well as music lovers who also enjoy and really high volume. Gamers, who need a high quality microphone will also be very pleased. In addition to the highly sensitive microphone with excellent sound and certainly appreciate the design of the microphone, which does not interfere in use. But that list of advantages headphones KALYPSO far from over. Headphones are two meters long, braided cable with volume control and microphone mute functions. The cable is in his mid-connected 4-pin audio jack, which offers great advantages. The primary function of this connection is compatible with your smarthpone when you use a headset for handsfree phone calls, or just listen to music. Also, it will never happen in the heat of the game that you would when sudden movement vandalized your headphones, because the cable is disconnected at this point. Headphones KALYPSO become your indispensable equipment.
Color: Black - Red
Drivers: 40mm
Microphone: Fixed
Volume control on cable
Cord: 2m, braided, 2x 3.5mm jack, combined 4-pin jack
Mikrofnu Sensitivity: -62 db ± 3 db
Impedance microphone: 2,2K?
Impedance converters 32?
Frequency range: 20 to 20 000 Hz
Sensitivity transducers: ± 110 db 4 db
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text: Homeweaetxdyvaydzcwq > Technology > Sustainability > EPA Releases New Water Data Sustainability Posted on: May 09, 2011 EPA Releases New Water Data By Rick Schwolsky —Rick Schwolsky INDOOR WATER USE IN HIGH-EFFICIENCY HOMES Showers emerged as the single largest source of indoor water use. ¬Weve got to get people to shorten their showers,® says study author William DeOreo, president of Aquacraft. ¬Low-flow showerheads [alone] arent doing it.® And while leaks account for 18% of indoor water use, few builders install leak detectors as part of their efficiency package. ¬We need to identify and then interfere with leaks,® DeOreo says. BENCHMARKS FOR EFFICIENCY Existing homes in the study consume an average of 190 gallons per household per day (gphd). High-efficiency homes can reduce that to less than 110 gphd by following WaterSense standards and limiting leakage to 20 gallons per day or less. COMPARISON OF INDOOR CONSUMPTION This chart shows improvements in indoor water efficiency comparing existing homes studied in 1999, ¬standard® homes built since 2001, and ¬high-efficiency® homes built to EPAs WaterSense standards. WATER USE AND OCCUPANCY As this chart demonstrates, per-capita indoor water efficiency improves as the number of occupants in a high-efficiency home increases. About the Author weaetxdyvaydzcwq Rick Schwolsky Rick Schwolsky, construction manager for the 2015 Greenbuild Unity Home, has worked in the residential construction industry for more than 40 years with a special focus on high-performance homes. Before joining Hanley Wood in 1993 as BUILDER's construction editor and later launching EcoHome magazine, he was president of Grafton Builders, a successful custom home building company in Vermont.


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Alto-Shaam 5011681 Stainless Steel Turkey Spit for AR-7E Electric Rotisserie Oven
Safely secure and cook whole turkeys and other poultry in your AR-7E electric rotisserie oven with the Alto-Shaam 5011681 stainless steel turkey spit! This turkey spit easily replaces one of the spits that came with your oven so you can thoroughly cook heavier foods to the perfect tenderness, moisture, and flavor.
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FLEXO Magazine : August 2008
FTA TODAY The Path to Excellence Do's and Don'ts for Winning an FTA Award By Greg Platt W hile FTA's Excellence in Flexography Awards competi- tion is intense, with numerous entries in each category and sub-category, there is a lot you can do to improve your score and help you win more awards and recognition for your company. Surprisingly, many involve following the most basic fundamentals of fl exographic printing. Contrary to some beliefs, judging is not solely subjective, but rather is quantifi ed by a scoring system. In each category, judges are divided into two groups of three, which rank the entries by the challenging nature of the image (degree of diffi culty) and the overall quality of the samples (level of execution). Within each of those two areas there are numerous points and factors to be con- sidered. The print samples that score well are those that cover all aspects of the judging criteria. SUBMISSION ERRORS An unfortunate number of very challenging and high-quality entries to the Excellence Awards end up scoring low due to sub- mission errors. The requirements for entry are very simple, and are in place in order to ensure that all samples are being judged fairly. A lot of it has to do with giving the judges a frame of refer- ence and/or a complete picture of the job itself. The most com- mon of these include: Failing to submit or meet the required number of repeats. In order to be considered for judging, every entry must have a mini- mum of four consecutive repeats. Judges must see that the image can, and has been, printed consistently. It also allows the three judges on either side of the table to examine the entry at the same time if they so choose, expediting the judging process. Failing to submit a target for color. Sure, if you didn't exactly match the proof the customer gave you, the entry will lose some points. But if the judges don't know what you are aiming for, they have no idea if you even came close. In fact, they'll have no choice but to assume as much. Lack of a target automatically brings with it a standard point reduction that makes it difficult to score high. 20 F LEXO AUGUS T 20 0 8 www. f l e x o g r a p h y. o r g Failing to submit the full web as described on the technical data sheet. Much like with the required number of repeats, the full web width must be available for the judges to score. Even if (or perhaps especially if ) the image is redundant across the width, you want to show that it was printed consistently. Failing to submit additional information that describes a diffi- culty in the project or with submissions. This is a no-brainer. Why wouldn't you want to explain what made a particular job diffi cult? While all the judges chosen every year are experts who understand and recognize a challenging graphic or vignette, there may be more to the story than they realize. Be as specifi c as possible. Many gold winners are not necessarily those that are the best executed, but rather are the best executed for that level of difficulty. Failing to enter the information correctly on the technical data sheet. Incorrect or contradictory information, whether intentional or otherwise, makes it tough for judges to fully understand the difficulty of a job, and thus poses a problem in the task of ranking all entries fairly. Double check your entry sheet before sending it in to FTA. FTA Awards Committee Chairman Gregg Platt (top left) and committee member Arleen Neustein (top right) answer a question for wide-web judges Mark Coffman (bottom left), Katie Tuckwiller (middle right) and Brian Jacobs (bottom right).
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Alright ladies and gentlemen we have arrived upon the final chapter of Glass Slipper. I started this story over a year ago and I definitely had a rough start with it but I want to thank everyone who followed me on this journey. It really does mean the world to me. This story may be over but I'll still be lurking around the site. I have a collab-fic that is a little sporadic with updating but it would make me so happy if you guys would check it out. "Armed and Dangerous" is literally a walk down memory lane which is perfect for the beginning of Season 5 wouldn't you say? A look attheir journey. I like journey stories (can you tell?). Anyways, that's where I'll be. I also have a few one-shots that I've been mulling over so you might see those in the following months. I don't really have any full on stories planned at the moment but I'll let you know.
Once again, thank you to everyone who favourited and alerted and especially reviewed, I am eternally grateful. And now, without further ado, the last chapter of Glass Slipper (warning: the following chapter may cause cavities. The writer is in no way responsible for any dental-related injuries sustained in the reading of this chapter).
Please review and as always you can follow me on twitter you can find me at vatrask
Enjoy 3
"Hey Cinderella." She rolled her eyes when he appeared behind her, his breath hot against her ear. When he first started doing that – and meaning to do it – it made her jump a little, but now it was just another Castle quirk that she loved to hate.
"You can stop calling me that now." She didn't even bother to look up from her writing.
"I'm sorry it's just so cool." He beamed, taking his usual seat pulled up beside her "I mean, we were like star-crossed lovers. We met and then you left and I looked for you and then years later I found you. It's very Cinderella."
"Yeah well we do have a fairytale theme going through our lives don't we?" She pursed her lips to keep from blushing; yes, she loved when he was cheesy and romantic almost as much as she loved when he spouted crazy theories that only sometimes worked.
"Oh yeah?" He leaned forward, playing along.
"Well there was the fairytale murder a couple of years ago; and the girl with Rapunzel syndrome last month. And I seem to recall you being my fairy godmother the first year we met."
"That dress was pretty hot."
"You should have seen it off." His elbow fell off the edge of the desk but he caught himself before he made a seen.
"Some things haven't changed." He grumbled, making a show of readjusting his jacket "you're still a shameless tease."
"I know," she smiled, scrunching her nose in that adorable way "but now I get to follow through with it when we get home."
He blinked at her, trying his best to look indignant but it just wasn't working with that 'deer-in-the-headlights' look. "Well maybe I have a headache."
"You never have a headache." She smirked, returning to her paperwork that probably could have been left later if it had been done sooner like she had intended. Damn that man and his adorable butt. She needed to focus on something else and since work was pretty much out of the question, she focused on her fairytale.
Rick was startled out of his thoughts as Kate laughed out loud, causing several other officers to look at her in confusion – Beckett didn't laugh. "Sorry," she slapped a hand over her mouth. "I was just thinking about that night." He gave her a confused smile until she elaborated. "I had kinda stolen my mom's diamond earrings for that night but I lost one when I was at the party and then I lost the other one somewhere." She sucked him into the past with her. "I wish I could find even one of them – to have something of my mom's that wasn't damaged in the explosion."
Rick was doing a horrible job of hiding a smile but she was still too lost to see him reaching into his pocket "you mean this?" She looked over, startled, when he produced a diamond ring from his pocket, not noticing the handful of officers who had stopped to watch the exchange.
"What's that?" Breathe, Kate, just breathe.
"It's an engagement ring."
"An engagement ring."
"Your engagement ring."
"I don't have an engagement ring."
"Want one?" BEATHE Kate, just breathe.
The room was eerily silent but she didn't dare take her eyes off of him. Still, she couldn't speak so Rick took a softer approach. "After you left the party, I found your earring in between the couch cushions. I kept it while I looked for you and then I found you. And when I realized I'd found you, I took it to a jeweler who removed the diamond and set it in a ring. If I had known it was your mothers I"
"Yes, Rick." He startled, blinking furiously for a moment before he found his voice.
"Yes." She was this close to bursting into laughter with the sheer delight that filled her but she kept it to a beaming smile, resisting the urge to just kiss him in the middle of the precinct. Oh what the hell, she threw her arms around him before he could comprehend what was happening. He stood with her still clinging to his neck, their lips fused together as she brought her weight completely down on him. He loved when she wore heels. It was like their first kiss in that alley all those years ago and yet it was something completely new – like their first kiss done over and over again – a tingling sensation that started in her toes and swept through her fingers that were running through his hair and ending with her buzzing head, dizzy with happiness. The shared in the humming joy between them until the sound of clapping wolf whistling became too overwhelming and they dropped back to reality. Splitting apart, they both blushed profusely but couldn't stop grinning.
They held their eyes as he slipped the ring onto her left hand and held her fingers warmly before gathering the strength to turn away and face the entire floor of the twelfth precinct. Just off to their right was Ryan and Esposito cat calling and taking pictures, their version of 'congratulations', no doubt texting Lanie who would be arriving on the floor any moment despite being several blocks away.
Kate hazarded a glance over to Captain Gate's office where Victoria was leaning against the frame with a slow smile on her lips, clapping slowly, seemingly approval in her eyes. She nodded her thanks to the Captain who echoed it with what could have only been a mischievous smile.
Wrapping her arms around Rick, she hugged him tightly, placing a light kiss on his cheek, not even trying to hide her smile anymore.
Rick held her hand as they walked through the lush green grass. She still had on her wedding gown and he his tux but neither of them cared. As they approached their destination, he squeezed her hand, compelling her to face him. "Are you sure about this?" She squeezed his hand back, staring into his eyes with love and determination, glassed over with tears.
"I'm sure." She nodded before tugging his hand behind her so he was following her as he'd always done. As they continued to tread through the grass, he kissed her ear causing her to duck her head to hide her shy smile, coming back just as quickly to catch his lips.
Before their kiss could become anything more than sweet she pushed forward propelling them the last few feet. "Come on, we have to get to the reception." He fell into her back when she paused in front of the tombstone and both of them fell into silence. Finally remembering to breathe, Kate reached out to caress the top of the stone with soft, gentle hands. "Hey Captain." She whispered so only Rick and the wind could hear her. Rick held her shoulders as she spoke to her –their- old friend. "We just wanted to stop by and say 'hi'. I know you would have wanted to be there today; it was a beautiful ceremony-"
"Only because she let me pay." Kate closed her eyes to laugh, hoping to expel any tears that may have formed knowing that Rick was so understanding of her need to do this.
"I swear Captain, he hasn't changed at all."
"Well that's a lie." He spoke to Roy. "I've changed so much because of this woman it's absolutely insane."
"You mean you're absolutely insane." She teased back, enjoying how comfortable this rather strange situation felt.
"Insanely in love maybe." She swatted his arm but kept her fingers on his, soothing away the sting.
"Rick," she half scolded before returning to her friend "sorry Captain; I guess not everything has changed." And then a thick silence fell over them as they listened to the summer wind dance around them, flashing them both back to that horrible day when they had almost lost it all. Closing her eyes, Kate sighed, expelling the last of her negative thoughts before turning her head to look at her husband. "Rick, do you have the envelope?" Without speaking, Rick reached into his pants pocket and produced a worn white envelope which he handed to his wife carefully. Caressing the envelope, Kate clutched it tightly in her hands before presenting it to Captain Montgomery. "This is for you, sir. It's the money you won on the 'relationship bet' about Castle and I." She shook her head, suddenly overcome with sadness. "You knew," she stated, her voice raw enough that Rick tightened his hold on her shoulders to steady her "from the very beginning you knew that we'd be together forever on this day at this time; with or without you." She could hear Rick sniffle once but wouldn't hold it against him as the tears flowed down her cheeks. After a moment to gather her strength she whispered, stroking her husband's hand with her thumb. "I owe you everything." He squeezed her shoulder as though to second the statement. "So I just wanted to say 'thank you' for bringing love back into my life and for teaching me how to be a better person. I'm where I am today because of you." She lowered the envelope until it drooped at her side, taking a deep breath of courage. "So, we're giving this bet money to your family; it's theirs." With that, Kate was so overcome with emotion that she could no longer speak. Her shoulders shook with her heavy sobs and so her husband took over.
"We just thought you should know that you won the bet; you were right all along." He spoke now in his own name. "Everything I have today I owe to you and so I am more than happy to put a little more bet money in their on behalf of Kate and I." He squeezed her shoulder as she continued to sob at her friend's grave. "We didn't even get the date right. I thought it would be later-"
"and I thought it'd be sooner." She breathed in admission, slowly regaining her composure. With a long, deep breath, Kate removed her hand from her shoulder to stroke the headstone one last time.
"Goodbye Captain." She whispered into the wind as Rick's hand came to her waist, slowly leading her away from Captain Montgomery. As they left the cemetery, Rick turned to look back at the grave on the hill, shining in the sunlight.
"Thank you." He mouthed for only Roy.
The reception was absolutely beautiful. It was quiet and quaint and with only their friends a family which was something Rick had decided on. Jim Beckett had been a little perturbed at first at not getting to pay for the wedding but Rick conceded to letting him pay for the reception and it was absolutely beautiful. She wasn't sure how it happened, she let Rick convince her to let him pick out their first song. She trusted him to make it romantic and wonderful but she was still a little anxious. However, when "so this is love" came blasting over the speakers in the ballroom, she bursting out laughing as Rick pulled her to him. The onlookers stood with knowing but bemused looks – only Beckett and Castle could be so casual and yet so loving as they danced their first dance as husband and wife.
"I love you Princess."
"I love you Writer."
"Hey, I'm supposed to be Prince Charming."
"Well looks can be deceiving."
"Hey." She silenced him with a peck on the lips "okay, I'll be your writer."
"You'll write us a happy ending right?"
"As someone completely invested in these characters, I'm going to say…mostly."
She lifted her head from his chest "mostly?"
"Well how boring would the story be if everything went right all the time? No I plan on having some ups and downs and wonderfully perfect moments that take your breath away. Can you handle that?"
"I think I can handle any twist you write."
"Really?" He suddenly dipped her out of the blue and she followed seamlessly, smirking up at him.
"You just wait to see what I have planned for you."
"I look forward to it."
So with laughter in their eyes, they spun around the floor as Cinderella and Prince Charming, having finally been reunited after so long.
And They All Lived Happily Every After, Kicking Ass and Being In Love.
The End.


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Excerpt: ...were laid on income, on wine, or on any other luxury, he would suffer no injury if by the taxation of necessaries, he should 319 be only called upon for the payment of 25l., as far as his own consumption of necessaries, and that of his family was concerned, but should be required to repeat this tax three times, by paying an additional price for other commodities to remunerate the labourers, or their employers, for the tax which they have been called upon to advance. Even in that case the reasoning is inconclusive: for if there be no more paid than what is required by Government; of what importance can it be to the rich consumer, whether he pay the tax directly, by paying an increased price for an object of luxury, or indirectly, by paying an increased price for the necessaries and other commodities he consumes? If more be not paid by the people, than what is received by Government, the rich consumer will only pay his equitable share; if more is paid, Adam Smith should have stated by whom it is received. M. Say does not appear to me to have consistently adhered to the obvious principle, which I have quoted from his able work; for in the next page, speaking of taxation, he says, "When it is pushed too far, it produces 320 this lamentable effect, it deprives the contributor of a portion of his riches, without enriching the state. This is what we may comprehend, if we consider that every man's power of consuming, whether productively or not, is limited by his income. He cannot then be deprived of a part of his income, without being obliged proportionally to reduce his consumption. Hence arises a diminution of demand for those goods, which he no longer consumes, and particularly for those on which the tax is imposed. From this diminution of demand, there results a diminution of production, and consequently of taxable commodities. The contributor then will lose a portion of his enjoyments; the producer, a portion of his profits; and the treasury, a...
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Book Description Penguin Books Ltd, 1971. Paperback. Book Condition: New. Bookseller Inventory # P110140400192
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Durable synthetic material that provides added protection for light duty jobs when using cleaning liquids, oils, or grease.
Helping You Build it Better®... Helping America Build it Better.®
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Other non affiliated stores are located in Amarillo, TX; Alexandria, LA; Fort Worth, TX, Lubbock, TX; Mineral Wells, TX and Nevada, MO.
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Located in a beautiful Haussmannian building, outstanding reception apartment decorated by the famous architect Charles Zana. It features a first spacious circular living room, a smaller living room, a dining room, a spacious fully-equipped kitchen and a bedroom. Also suitable as a family apartment, featuring a master suite with a bathroom and a walk-in closet, and three other ensuite bedrooms with their own bathroom. An area may be converted into a fifth room. A studio apartment and two cellars may be acquired at extra cost. A rented parking space in the neighbourhood.
Paris 16th District — La Muette
Price on request
Total area: 400 m² 4 bedrooms
- Built in 1905
- 3 floor
- Total rooms: 9
- Car parks
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wall tangential temperature gradient
Hi all,
Does anyone know how to apply a temperature gradient along the walls of a simple channel (so wall temperature varies in the streamwise direction). I'm looking at using rhoCentralFoam to simulate a surface temperature gradient driven gas flow. Can I manage this with groovyBC?
What you want is a boundary condition with a variable value depending of the position ?
I don't know if OF has a derived boundary able to do this. Usually people use the "swak4foam" library to perform this kind of special boundary. You can use the groovyBC, google will help you.
The official website is here : http://openfoamwiki.net/index.php/Contrib/swak4Foam
And an example is here : http://openfoamwiki.net/index.php/Contrib_groovyBC
Thanks Frédéric.
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Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
Long time ago, I was arrested by ICE and had to pay a bond to get released. The person who posted the bond now lives outside the country and he wants to give me power of attorney so that I can go to ICE local office to ask for the money. Can he give me power of attorney if he is outside the country? Does he have to go to the consulate office to process the power of attorney paperwork?
He probably can grant you power of attorney, but he would likely have to get his signature notarized at the US Consulate in the country where he is now living. The other matter is whether you complied fully with the terms of the bond; if not, the bond may have been breached and the money forfeited. Aside form checking with the local ICE office, consulting with an experienced immigration attorney may help.
Good luck!
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Light shelf wear and minimal interior marks. Bookseller Inventory #
Title: Days Woe Nostradamus prophecy of 400 years ...
Publisher: China City Press
Binding: Paperback
Book Condition: Good
Book Description paperback. Book Condition: Good. Ship out in 2 business day, And Fast shipping, Free Tracking number will be provided after the shipment.Days Woe Nostradamus prophecy of 400 years ago. the collectionFour Satisfaction guaranteed,or money back. Bookseller Inventory # EH053895
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Taylor Swift and 'Teen Mom': The Unlikely Connection!
For example, does America's music sweetheart have in common with Teen Mom?
She loves it!
"Teen Mom, 16 and Pregnant, 90's one-hit wonders, watching YouTube videos of kittens, the app CatPaint . . I CatPaint all my pictures. It's weird," Taylor responds when asked what her pop-culture guilty pleasures are.
Still, it's another TV icon that really gets Swift excited: Detective Olivia Benson (played by Mariska Hargitay) from Law & Order: SVU. "I sit in my apartment and watch hours of that show," Taylor says when asked if she could have dinner with any character, who it would be. "I sort of feel like me and Olivia are BFFs."
Fictional characters aside, the country crooner feels perfectly content with the crowd she has surrounded herself with. "My friends all have different jobs — some I get to see at award shows, some I see when they're home from college, some are in my band! I'm so lucky to have so many people I love and trust."
Check out behind the scenes footage of Taylor's cover shoot right here, and scope the article for more of Swift's favorite things.


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- 01.11.16
See How Much Your Favorite Disney Characters Changed from Concept Art to Final Frame
Knowing what Dumbo, Bambi, Robin Hood, and more could've looked like cannot be unseen.
Disney is marking the release of it upcoming 55th animated feature film Zootopia with a trip inside its historic vault.
In honor of the Zootopia's anthropomorphic adventures hitting screens March 4, the Disney blog Oh My Disney compiled the concept art of your favorite animal characters.
Some of the original art doesn't fall far from the final product: Lady from Lady and the Tramp is a dead-ringer for her throwback, and Bambi is just as doe-eyed as ever. But some drawings will make you eternally grateful for second and third drafts, e.g. Sebastian going from an under-the-sea horror show to cuddly crab.
Check out all of the before-and-afters in the slide show above.


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by Palram
Order now to get it by: Saturday July 29 - Monday July 31
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Tuesday, September 29, 2015
Bee Smalltalk
The prerelease of Bee Smalltalk from 2014 is still not accessible on GitHub as it was stated in the announcement. So I thought the project is dead. But now a new blog post appeared. Looks like there is still activity. Looking forward in trying out.
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|Darren Michaels
|First appearance
|4th August 2000
|Last appearance
|4th March 2001
|Number of appearances
|Played by
| Bruno Langley (2000)
Nicholas Zabel (2001)
Darren Michaels was a short-term boyfriend of Candice Stowe who dated between 2000 and 2001. Candice turned up with Darren at 8 Coronation Street in August 2000 in order to attend Bethany Platt's christening.
In March 2001 he dumped Candice when he found out that she was also dating Todd Grimshaw and he told Todd to get rid of her as she was a two-timer.
- Darren was played by Bruno Langley, who five months later would debut in the regular role of Todd Grimshaw. Upon Darren's next appearance in March 2001 he was recast as Nicholas Zabel, and with somewhat uniqueness to the show, Todd appeared alongside Darren, a character Langley previously played.


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Accommodations in DéniaSee All
This is a comprehensive travel guide for Dénia in Spain. From budgeting tips to finding the best spots, this travel guide has got all the insider information to explore Dénia like a local. When planning a trip to Dénia, make sure you visit its near-by prime city of Ibiza. Take a short trip from Dénia and set some time aside to explore the Ibiza. Dotted with places like Dalt Vila, Pacha Ibiza, Es Vedra; it will definitely be a good idea. A holiday in Dénia can generally be of 1-2 days.
Dénia is an affair to remember, follow this apt itinerary of the place and return home with a bunch of memories.
Explore the best journey planner for your visit to Dénia and create a flawless plan in few simple steps!


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Walter Ben Hunt
Walter Ben Hunt (1888-1970) was an important figure in outdoor recreation and the revitalization of skills and crafts from the pioneer era. His writings include more than 20 books and 1,000 magazine articles, including those written under the pen names Lone Eagle and Whittlin' Jim for Boys' Life Magazine.
Books from Blair
Rustic Construction: Making Furnitures, Fixtures & Outdoor Structures Using Bark, Branches & Slab Lumber (Bright Mountain Books)


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Christa Severns Archives
Phoenix IDA announces new board members and officers
The Phoenix IDA, a leading industrial development authority in Arizona, announces the election of new Board officers for 2017 and the appointment of two new Board members. The Phoenix IDA's Board of Directors' new officers include: · Sal Rivera, president; partner and shareholder, Rivera Law Group, P.C. · Barbara Ryan Thompson, vice president; executive vice… Read More →
Hall joins CTCA's Board of Directors
Cancer Treatment Centers of America® (CTCA) at Western Regional Medical Center announced Arizona Diamondbacks President and CEO Derrick Hall will be joining its Board of Directors. "As a prostate cancer survivor, this is a cause very close to my heart," said Diamondbacks President and CEO Derrick Hall, who recently launched his own non-profit organization, the… Read More →
Phoenix IDA Names 2013 Board Officers
The Phoenix IDA, a prominent industrial development authority established in 1981 to assist economic and community impact enterprises, announces the election of its 2013 board officers. Bruce Mosby is president of the board; Donald Keuth is vice-president; Tommy Espinoza is treasurer; and Christa Severns is secretary of the Phoenix IDA board for a one-year term… Read More →


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Wednesday, July 23rd, 2014
2 colors on heavy stock cold press paper. 13" x 19" ltd edn of 150 signed and numbered by the artist.
Tuesday, July 8th, 2014
We are really psyched to be able to put out Night Watch Zine, and are always amazed by the radical art that all the contributors come up with, no matter what demented new theme we throw at them. When we are putting the zine together and get a new entry from one of our contributors it is always a complete surprise and a really exciting moment, to see what insane art they've created. We are so pumped on all the hard work our contributors put into their Night Watch page, and if you are really into what they've created too, well then go follow them and buy their merch! Us shitheads gotta stick together and support each other, and that is what Night Watch is all about! So check all these talented guys and gals out!
Wednesday, July 2nd, 2014
Here's your chance to win the original pen and ink illustration for Bloodlines AND you get a 13" x 33" Ltd Edn giclee print signed and numbered by the artist. There are also six hand painted wooden eyeballs placed in random print orders. Its a win-win!
Wednesday, July 2nd, 2014
Lots of new shirts this week with that signature Jondix black and gold. We've got some hoodies too for those cool summer nights.
Wednesday, July 2nd, 2014
Stash your stuff in these awesome canvas tote bags by Virginia Elwood. Have a lot of stuff? Get one of each.
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Brand: Rolex Model #: REF 168622 Serial #: A****** Series: Yacht-Master Movement: Automatic Case Material: Steel Case Shape Round Case Diameter 35mm Crown Screw Down Crystal : Sapphire Bezel Platinum Dial: Gray with white markers Bracelet: Steel. All links. Clasp: Deployment Date at 3 o'clock Box & Papers Guaranteed Authentic, like new condition.
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The event title says it all… Come down to Radio Bean at 10pm every Tuesday to get your weekly honky tonky fix with Eric George and friends!
- This event has passed.
January 17 @ 10:00 am - 11:55 pm
|Recurring Event (See all)
Weekly on the same day until February 2, 2017


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Molecular basis of a novel adaptation to hypoxic-hypercapnia in a strictly fossorial mole
Elevated blood O2 affinity enhances survival at low O2 pressures, and is perhaps the best known and most broadly accepted evolutionary adjustment of terrestrial vertebrates to environmental hypoxia. This phenotype arises by increasing the intrinsic O2 affinity of the hemoglobin (Hb) molecule, by decreasing the intracellular concentration of allosteric effectors (e.g., 2,3-diphosphoglycerate; DPG), or by suppressing the sensitivity of Hb to these physiological cofactors.
Here we report that strictly fossorial eastern moles (
We propose this Hb phenotype illustrates a novel mechanism for adaptively elevating the CO2 carrying capacity of eastern mole blood during burst tunnelling activities associated with subterranean habitation.
Among mammals that are adapted to hypoxic environments, only subterranean species are also obliged to breathe air with elevated concentrations of carbon dioxide
Figure 1
Phylogenetic hypothesis of the Family Talpidae
Phylogenetic hypothesis of the Family Talpidae. (A) Shinohara et al. 2003
In order to assess the functional and evolutionary adaptations of mole Hbs, and their mechanistic basis, we determined the coding sequences of the adult-expressed α-and β-type globin chains of coast mole (
Hb-O2 binding properties
Both gel electrophoresis and isoelectric focusing (IEF; inset of Figure
Figure 2
Values of PO2 and Hill's cooperativity coefficient at half oxygen saturation (P50 and
Values of PO2 and Hill's cooperativity coefficient at half oxygen saturation (P50 and
Oxygen equilibration curves revealed striking functional differences between the Hbs of the two species (Figure
Table 1
Heterotropic effects at half-saturation (P50) for coast and eastern mole hemoglobin
Eastern mole
Coast mole
Hb I
Hb II
Hb I
Hb II
Δlog P50/Δlog [Cl-]1,2
Δlog P50± 2.5 mM DPG1,3
Δlog P50/ΔpH4
25°C, 0.1 M Cl-
37°C, 'stripped'
37°C, 0.1 M Cl-
37°C, 0.1 M Cl- + 2.5 mM DPG
Oxygenation enthalpy; Δ
1pH = 7.2
2[DPG] = 0 mM
3[Cl-] = 0.1 M
4over pH range 6.9-7.4; PCO2 = 0 mm Hg
5[Cl-] = 0.1 M; [DPG] = 0 mM; pH = 7.2
O2-binding properties of whole blood
In accordance with the observed differences in the oxygen affinity of coast vs. eastern mole Hb components in the presence of allosteric effectors (Figure
Figure 3
Oxygen equilibration curves of freshly drawn coast (■) eastern (▲) and star-nosed mole (●) blood at 36°C and a PCO2 of 38 mm Hg
Oxygen equilibration curves of freshly drawn coast (■) eastern (▲) and star-nosed mole (●) blood at 36°C and a PCO2 of 38 mm Hg.
Table 2
Respiratory characteristics of eastern, coast and star-nosed mole whole blood
ΔlogP 50/ΔpH 4
Δlog P 50 /ΔT
PCO 2 = 38 mm Hg
PCO 2 = 38 mm Hg
PCO 2 = 38 mm Hg
PCO 2 = 38 mm Hg
P d 2
P d
Eastern mole
Coast mole
Star-nosed mole
1P50 (mm Hg) = PO2 corresponding to 50% oxygen saturation
2Pd (mm Hg) = P50 × ((
4CO2-Bohr effect; pH varies by CO2 titration
5Overall heat of oxygenation.
Prominent differences were also detected in specific hematological parameters of the two fossorial species, with the most significant being the > 15 fold lower DPG levels in the erythrocytes of eastern mole relative to coast mole (Table
Table 3
Hematological parameters, skeletal muscle myoglobin concentrations and buffering capacities (± 1 SE) of coast and eastern moles (samples sizes are indicated in brackets)
Eastern mole
Coast mole
Hematocrit (%)
56.4 ± 1.3 (7)†
46.8 ± 2.0 (11)1
Hemoglobin (g dL-1)
19.2 ± 0.7 (7)
17.4 ± 0.8 (11)1
RBC (106/mm3)
12.58 ± 0.32 (3)
10.48 (1)
MCV (μ3)
46.0 ± 1.3 (3)
42.6 (1)
MCH (pg)
15.3 ± 0.6 (3)
13.5 (1)
MCHC (g L-1)
333 ± 7 (3)
318 (1)
2,3-DPG (mM L-1 RBC)
0.45 ± 0.06 (4)†
7.09 ± 0.20 (3)
Myoglobin content (mg g-1 wet tissue)
8.34 ± 0.38 (6)
9.24 ± 0.28 (7)1
10.98 ± 0.64 (6)
12.10 ± 0.25 (10)1
8.56 ± 0.21 (6)†
10.61 ± 0.56 (10)1
Buffering capacity, β (Slykes)*
43.86 ± 3.29 (6)
37.33 ± 2.10 (10)1
44.99 ± 5.54 (6)
38.94 ± 1.77 (10)1
Plasma pH (pH
7.42 ± 0.01 (6)
Intraerythrocytic pH (pH
7.21 ± 0.00 (6)
Plasma osmolarity (mOsm)
333 ± 2 (6)
†Interspecific differences are significant (
*1 Slyke = μmoles of base required to titrate the pH of 1 g of wet muscle by 1 pH unit
1data from
Identification of Hb isoforms
One β-like and two α-like globin cDNAs were obtained from three separate eastern moles, while single α- and β-like globin cDNAs were obtained from three coast moles examined (Figure
Figure 4
Alignment of mole α- and δ-globin chains
Alignment of mole α- and δ-globin chains. Amino acid residues of coast and eastern moles were deduced from cDNA nucleotide sequences and are shown only at positions where they differ from those of European moles
Phylogenetic analyses of mole β-like genes
Phylogenetic surveys of the β-globin gene family have revealed that, in most mammalian species, the β-type globin chains of adult Hb are encoded by one or more copies of the
We used phylogenetic reconstructions of coding sequences and intron 2 sequences to determine whether the β-like globin genes of eastern mole and coast mole are orthologous to the
Figure 5
Phylogenetic analysis of mole β-like globin genes
Phylogenetic analysis of mole β-like globin genes. Maximum likelihood phylograms depicting relationships among mammalian β-like globin genes based on (A) 477 bp of coding sequence, and (B) 938 bp of intron 2. With the exception of the sequences from eastern mole (
The pioneering work of Bunn
Though δ5Ala is present in eastern (and coast) moles, contrary to our expectations, all known phosphate-binding sites are conserved in the β-type δ-chains of moles (Figure
In accord with this suggestion, our structural model illustrates that the carboxyl side chain of δ136Glu forms a stable salt bridge with the nearby ε-amino group of δ82Lys (Figure
Figure 6
Molecular models of the central cavity between the two δ-globin chains of (A) coast and (B) eastern mole hemoglobins
Molecular models of the central cavity between the two δ-globin chains of (A) coast and (B) eastern mole hemoglobins. In coast mole deoxyhemoglobin, this cavity is lined by 8 cationic residues (δ1Val, δ2His, δ82Lys and δ143His) that form competitive binding sites for anions (Cl-, lactate and DPG) that stabilize the low O2 affinity T-state conformation. In eastern mole deoxyhemoglobin, the carboxylate side chain of δ136Glu forms a salt bridge with ε-amino group of δ82Lys of the same chain, thus deleting key docking sites for these anions, and reducing competition for CO2 binding (carbamate formation) at the N-terminus (δ1Val). This change is expected to markedly increase the maximal CO2 carrying capacity of eastern mole blood.
The chloride sensitivities of both major Hb components of the eastern mole (0.21-0.25) are about 30% less than those of coast mole Hbs (Table
The 7.3 kJ mol-1 O2 difference in overall oxygenation enthalpy of eastern mole whole blood relative to coast mole blood (Table
The negligible effect of DPG on eastern mole Hb (Table
We hypothesize that the loss of DPG binding sharply increases the carrying capacity of eastern mole Hb for the metabolic end product CO2, thus providing a strong selective advantage for this subterranean inhabitant. Unlike carbon monoxide, CO2 does not bind to the heme iron, but instead can interact with the uncharged α-amino termini of the four globin chains to form carbamino CO2
Within the erythrocytes of systemic capillaries, CO2 is largely hydrated to HCO3
- and H+ by carbonic anhydrase. HCO3
- is subsequently exchanged with Cl- via the band 3 protein anion-exchanger, and transported in the plasma
The capacity of certain mammals to withstand low-oxygen environments is thought to largely reside in the enhanced binding affinity of their blood Hb for oxygen. Here we document that following an extensive period of subterranean evolution, the lineage leading to present day eastern moles adopted a low oxygen affinity, DPG-insensitive blood phenotype - providing the first demonstration of this phenomenon in any mammal chronically exposed to hypoxia (and only the fourth known origin of this phenotype among mammals). The primary molecular mechanism involves an amino acid substitution (δ136Gly→Glu) that forms a salt bridge with δ82Lys of the same chain, thus deleting key binding sites for allosteric effectors (DPG, Cl- and lactate) between δ1Val-δ82Lys and markedly reducing competition for CO2 binding (carbamate formation) at the N-terminus. Accordingly, we suggest this unique Hb phenotype enhances CO2 carrying capacity during burst activity (tunnelling) in gas-exchange impeded burrows.
Blood/tissue collection and preparation
All study animals were captured and cared for in accordance with the principles and guidelines of the Canadian Council on Animal Care under the authorization of a University-approved animal research protocol (University of Manitoba Animal Use Protocol# F01-026). Blood samples (~2 ml) were collected from anaesthetized eastern (Nashville, Tennessee) and coast moles (Abbotsford, BC, Canada) and immediately stored at -80°C. Samples were subsequently thawed and diluted with 1 volume distilled water, 0.1 vol. 1 M HEPES buffer (pH~7.5), and centrifuged for 15 min at 14,000 RPM. Individual isoHb components were isolated by preparative isoelectric focusing in a 110-ml LKB column containing a 1% solution of CO-saturated ampholines (pH range 6.7-7.7; LKB, Sweden) and eluted 1-ml fractions were analyzed for absorption (540 nm) and pH. Pooled fractions of individual Hb components were dialysed for 24-36 h against three changes of CO-equilibrated dialysis buffer (0.01 M HEPES), concentrated by ultrafiltration and frozen at -80°C in small aliquots that were thawed individually on ice prior to analyses. Air equilibrated samples showed slight if any spectrophotometric evidence for oxidation (< 5%).
Whole blood
Blood samples (~2 ml) were obtained in heparinized syringes via cardiac puncture from a single anaesthetized star-nosed mole (Caddy Lake, MB, Canada) and from coast and eastern moles collected from the same areas, but in different years, as those described above. A small sub-sample (60 μl) of blood was immediately transferred to a pre-heated tube (36-37 °C) and pH determined using a IQ Scientific benchtop ISFET pH meter with PH16-SS stainless steel micro pH probe.
Forelimb, hindlimb and heart muscles from six additional eastern moles euthanized with an overdose of Isoflurane inhalant anaesthetic were immediately dissected, freeze-clamped in liquid N2, and stored at -70°C. Tissue Mb and buffering capacity were later determined following the methods of Reynafarje
Oxygen binding measurements
Immediately before O2 equilibration determinations, appropriate volumes of water, 0.1 M HEPES buffer, and when applicable, standard KCl and 2,3-DPG solutions were added to aliquots of purified Hb components (final Hb4 concentration 0.05 mM). Oxygen equilibration data were measured in duplicate at 25 and 37°C via absorbance changes at 436 nm using a modified diffusion chamber technique
Whole blood
Blood-oxygen binding properties were measured by equilibrating small (3-4 μl) aliquots of whole blood to O2 tensions ranging from 0 to 190 mm Hg using a modified
DNA/RNA extraction and cDNA library construction
Genomic DNA was prepared from 100-200 mg of spleen or liver tissue using standard phenol/chloroform extraction procedures. Primers were designed using areas of high sequence identity in the coding, and 5' and 3' flanking regions of orthologous eutherian α- and β-like globin genes and from the published amino acid sequences for these polypeptide chains of the European mole
Additional file 1
Table S1. Primers used to amplify the
Click here for file
Total RNA was extracted from ~80 mg of eastern (
Clone selection
Positive clones were selectively retrieved from the plasmid library using a modified version of the magnetic bead cDNA capture method described by Shepard and Rae
Additional file 2
Table S2. Oligonucleotides used to probe the
Click here for file
Selected clones were screened for the presence of their respective insert by scraping an isolated colony into a 0.2 ml PCR tube and adding 15 μl of a PCR reaction mix (0.35 μl of each dNTP (2.5 mM), 1.5 μl of 10 × Reaction Buffer (Invitrogen), 0.6 μl of 50 mM MgCl2, 0.6 μl of each primer (designed from highly conserved regions among mammalian α- and β-globin genes using Primer Premier 5.0 software), 0.12 μl (0.6 Units) of Taq DNA polymerase (Invitrogen) and 10.18 μl of ddH2O). Positive clones were used to inoculate 8 ml of LB culture medium containing kanamycin (50 μg ml-1 final concentration) and incubated for 18 h at 37°C while shaking at 225 rpm. A 3 ml sample of each culture was purified using a QIAprep® Spin Miniprep Kit (Qiagen). 2 μl of purified plasmid DNA was digested with Eco RI and Hind III (1 μl of each enzyme, 2 μl of 10 × React®2 buffer and 14 μl of ddH2O) and electrophoresed for 1 hr at 100 V on a 1% agarose gel (UltraPure™, Invitrogen) to confirm the size of the insert.
DNA sequencing
Sequencing reactions were preformed on 200 ng of purified plasmid DNA using the BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and the universal sequencing primer M-13(R). Reaction mixtures were sequenced using a 4-capillary Applied Biosystems 3130 Genetic Analyzer. Consensus alignments for each gene were constructed using Sequencher™ (Version 4.6) software, and the amino acid compliment of each globin chain was deduced. Sequence data were deposited in GenBank with the accession numbers (
Determination of Hb isoform composition
After using isoelectric focusing native gels to assess Hb isoform diversity, the individual globin chain subunits were dissociated and separated by means of Acetic acid-Urea-Triton X-100 (AUT) gel electrophoresis
Phylogenetic reconstructions
To infer orthologous relationships of β-like globin sequences from eastern mole and coast mole, we conducted a phylogenetic survey of nucleotide variation in the β-like globin genes of six other eutherian mammals. In addition to eastern mole and coast mole, this set of species included three other eulipotyphlan species (
Molecular modeling
Amino-acid substitutions of both mole species (relative to human Hb A) were inserted into the 3D T-state deoxy structures of human Hb with DPG absent (eastern mole model from PDB
Authors' contributions
KLC and REW designed research; KLC, JFS, JS, AVS, AP and JB performed research; KLC, JS, JFS, HM, KC, JB and REW contributed new reagents/analytic tools; KLC, JS, AVS, JFS and HM analyzed data; KLC drafted the manuscript; and KLC, JFS and REW contributed to the final manuscript writing and its revisions. All authors have read and approved the final manuscript
We thank R. Gusztak and S.T. Sheehan for aid in procuring blood samples and A. Bang for technical support. M. Krogh Larsen, A. Sloan and J. Howatt provided valuable assistance with gene sequencing, while N. Lovejoy, G. Valdimarsson and O. Westergaard kindly provided lab space and equipment for components of this work. This research was supported by grants from the Natural Sciences and Engineering Research Council (NSERC) of Canada (KLC, JS), the National Institutes of Health (JFS), the National Science Foundation (JFS), the Nebraska Tobacco Settlement Biomedical Research Development Fund (HM), the Danish Natural Science Research Council (REW), and the Carlsberg Foundation (REW).
The ecological significance of certain new haematological findings in the mole and hedgehog
Concentrations of carbon dioxide and oxygen in mole tunnels
Comparative studies of the respiratory function of mammalian blood. V. Insectivora: shrew, mole and nonhibernating and hibernating hedgehog
Adaptation of hemoglobin function to subterranean life in the mole,
Die primärstruktur des hämoglobins des maulwurfs (
Molecular phylogenetic relationships of moles, shrew moles, and desmans from the new and old worlds
Phylogenetic relationships with the Family Talpidae (Mammalia: Insectivora)
Comparative mycology of moles and the phylogeny of the Talpidae (Mammalia, Lipotyphla)
From the Arctic to fetal life: physiological importance and structural basis of an 'additional' chloride-binding site in haemoglobin
Blood oxygen affinity and alveolar ventilation in relation in body weight in mammals
Mechanism of deamidation in haemoglobin Providence Asn
Posttranslational deamidation of proteins: the case of hemoglobin J Sardegna [α50(CD8)His→Asn→Asp]
Differential loss of embryonic globin genes during the radiation of placental mammals
Origin and ascendency of a chimeric fusion gene: the β/δ-globin gene of paenungulate mammals
Hedgehogs, shrews, moles, and solenodons (Eulipothyphla)
Differences in the interaction of 2,3-diphosphoglycerate with certain mammalian hemoglobins
Species adaptation in a protein molecule
A novel allosteric mechanism in haemoglobin: structure of bovine deoxyhaemoglobin, absence of specific chloride-binding sites and origin of the chloride-linked Bohr effect
Regulation of oxygen affinity of mammalian haemoglobins
Abnormal functional properties of Hb Hope (α2β2136(H14)Gly→Asp)
Hb Hope, β136(H14)Gly→Asp, in a diabetic Japanese female and its functional characterization
Molecular pathology of human haemoglobin
The carbon dioxide affinity of various human hemoglobins
Hemoglobin Hope: studies of oxygen equilibrium in heterozygotes, hemoglobin S-Hope disease, and isolated hemoglobin Hope
Hemoglobin Hope: a beta-chain variant
Determination of the pK values for the alpha-amino groups of human hemoglobin
The carbamate equilibrium of α- and ε-amino groups of human hemoglobin at 37°C
Hemoglobin Raleigh (β1 valine→acetylalanine). Structural and functional characterization
Hb Fukuoka [β2 (NA2) His→Tyr]: a new mutation at the 2,3-diphosphoglycerate binding site
Hemoglobin Little Rock (β143 (H21) His→Gln). Effects of an amino acid substitution at the 2,3-diphosphoglycerate binding site
Anionic control of function in vertebrate hemoglobins
Hemoglobin Rahere, a human hemoglobin variant with amino acid substitution at the 2,3-diphosphoglycerate binding site. Functional consequences of the alteration and effects of bezafibrate on the oxygen bindings
Hemoglobin Providence. Functional consequences of two alterations of the 2,3-diphosphoglycerate binding site at position beta 82
Structure of human foetal deoxyhaemoglobin
Hemoglobin: Structure, function, evolution, and pathology
The reaction between diphosphoglycerate and hemoglobin
Oxygen transport in the blood of arctic mammals: adaptation to local heterothermia
Hemoglobin structure and function
Hemoglobin: Molecular, Genetic and Clinical Aspects
Mammals in the highlands of southern Peru
Seasonal changes in haematological and respiratory properties of muskrat (
The specific influence of carbon dioxide and carbamate compounds on the buffer power and Bohr effects of human haemoglobin solutions
Specifically carboxymethylated hemoglobin as an analogue of carbamino hemoglobin. Solution and X-ray studies of carboxymethylated hemoglobin and X-ray studies of carbamino hemoglobin
Carbon dioxide transport in alligator blood and its erythrocyte permeability to anions and water
Body oxygen stores, aerobic dive limits and diving behaviour of the star-nosed mole (
The life history and ecology of Townsend's mole
Simplified method for the determination of myoglobin
Buffering capacity of vertebrate muscle: correlations with potentials for anaerobic function
Preferential hemolysis of postnatal calf red cells induced by internal alkanlinization
Veterinary hematology
Use of ionic and zwitterionic (Tris/BisTris and HEPES) buffers in studies on hemoglobin function
Thin-layer methods for determination of oxygen binding curves of hemoglobin solutions and blood
Fasting metabolism and thermoregulatory competence of the star-nosed mole,
Simple, accurate equations for human blood O2 dissociation computations
Magnetic bead capture of cDNAs from double-stranded plasmid cDNA libraries
Hb F-Porto Torres [Aγ75(E19)Ile→Thr, 136(H14)Ala→Ser]: a novel variant of theAγ chain having two substitutions, one being that of Hb F-Sardinia
Protein-protein interactions of tandem affinity purification-tagged protein kinases in rice
Conserved sequences of sperm-activating peptide and its receptor throughout evolution, despite speciation in the sea star
MUSCLE: multiple sequence alignment with high accuracy and high throughput
TREEFINDER: a powerful graphical analysis environment for molecular phylogenetics
A biomolecular force field based on the free enthalpy of hydration and solvation: The GROMOS force-field parameter sets 53A5 and 53A6
DINO: Visualizing Structural Biology


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The 2014 NBA season has come to an end, and the King has been slain at the hands of the San Antonio Spurs. It was not the most exciting of playoff series. In fact, it was a rather one-sided thrashing delivered by a team that should be considered among one of the best squads of all time, certainly the best since the Michael Jordan era of the 1990s. But though the games may have not been full of buzzer beaters and dunks, the relevancy of the Spurs' run at the title will echo throughout the expansive halls basketball history due to the unique nature of the team.
For the Spurs, this season's title was all about redemption. The 2013 NBA Finals will always be remembered as the most devastating defeat in Spurs history. With a 3-2 lead against Miami, San Antonio was well on their way to being crowned the champions. They beat the Heat with mere seconds left in the game, and the trophy was (literally) within sight. And then Ray Allen happened, and the veteran guard's last minute heroics turned the tide toward Miami. After losing the series to the Heat many thought that it would be the last time we would see the likes of Tim Duncan, Manu Ginobili, Tony Parker, or Greg Popovich in the NBA Finals. The team was aging, and it would take a miracle to bring them back from so devastating a defeat. That miracle happened only one year later, and the wounds left from last season's heartbreak are healed and forgotten.
What makes the Spurs a special team is that we will never see anything like them again in sports. The legacy of San Antonio is one of a familial group that refuses to break apart. Duncan, Parker, and Ginobili all took large pay cuts this season just so that the franchise would have enough money to keep them all together. Ginobili and Parker have played on the ball club for 12 and 13 years respectively, and Duncan and Popovich have been winning championships together throughout the last 17 years. When most NBA teams would have traded away their aging stars upon the first sight of turmoil and underachievement, San Antonio stuck together, worked through their problems, learned from them, and became even more dangerous. In essence, the Spurs are the professional embodiment of every high school or college coach's dreams: close-knit, selfless, tireless, and emphatically successful.
So where do the Spurs go from here? Their stars are all well into their 30s, and basketball is without question a young man's sport. But if the Spurs maintain their methods of team building, young players such as Danny Green and Kawhi Leonard will surely carry the torch once the Spurs' big three begin to fade. San Antonio has not only found a way to stay dominant in old age, but they may have found a way to build a true team-oriented program, one that is not dependent on a blockbuster player who is the face of the team, but instead relies on the core values of selflessness and discipline. If the Spurs keep up with this methodology and philosophy, they should enjoy success for decades to come.


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LG Plumbing Ltd is a plumbing and heating business based in Battersea, South West London.
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Luke Gower


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147 total
My employer wants to settle with me, however they still have not responded to unemployment. Does my signing their settlement agreement keep them giving me unemployment? They gave me 3 weeks pay but I am going to need unemployment still. I'm confus...
I agree with the first responder that you should consult with a Worker's Comp/Employment Law attorney regarding your situation. Be sure to take all of the documents with you when you meet with the attorney before signing any agreement. Then the attorney can review the entire documents and advise you accordingly.See question
I live in Gunnison colorado. I tried in Florida 3 times and denied and have a court date in feb in Florida. Like to see if I can transfer things here
As was stated by the other responder your question is a little unclear. What did you try to do in Florida three times? If you have a court date in Feb. in Florida and you want to transfer the case here you need to meet with an attorney with all of your documents to discuss your matter; however, in light of the fact that your court date is coming up in 3 months the likelihood of being able to transfer the case to Colorado, even if you have good grounds is probably remote or unlikely. Hopefully you have an attorney who is handling your lawsuit in Florida.See question
I have keys to box, retirement account that I am 100% benificiary. I also have 6 siblings. Nobody wants to deal with this. With no durable POA can I do anything?
I agree with Paul's response. Since you can't find the original Will, if your Dad did not have a Trust then someone will have to file a probate action with the court in the county where your Dad was living. You should try to come to an agreement with your siblings and your mother, if she's still alive and was still married to your Dad when he passed about trying to locate the Will and/or filing a probate action. If everyone can agree then the process will be less costly. You should consult with a probate attorney, ideally in the Pueblo area or the county where your Dad was living at the time of his death.See question
My brother and I were appointed co-personal representatives of our mothers estate. I was talked in to letting him be the administrator. This was when he took over all decision making from color of carpet to painting the garage floor. My thoughts...
I agree with the prior response that it is always best if you can work things out with your brother, the Administrator of your Mom's estate. That would certainly be the least expensive way to resolve your differences. However, if you cannot and believe that he is acting inappropriately you can request the court to appoint another as the Administrator. But it won't be inexpensive and obviously it will put you in an adversarial position with your brother and potentially any other beneficiaries. To contest your brother's appointment would probably require you to hire a probate/Estate Planning attorney to help you with the process.See question
I'm trying to help my mom out. Dad died leaving part of his estate to my mom and part to a charity. My mom and dad each kept some of what they own in their own name and some was owned jointly, in both names. Dad left my sisters and I out of his ...
In addition to the answers provided above it would be a good idea for you to take a copy of your Dad's Estate Planning documents and a copy of the banking documents listing you and your sister as beneficiaries to an Estate Planning attorney to get a definitive answer. Your mother should probably do the same as your Dad's estate may need to be probated.See question
I posted a cash bail and am afraid fool I bailed out is going to flee the state. The clerk of courts referred me to the proper forms- but what is the burden of proof I need to get the bail back?
You probably don't have a burden of proof to convince the judge that s/he should make the Defendant post a substitute bail or allow the Defendant to go on a Personal Recognizance (PR) bail, meaning the Defendant is on their word that they will show up in court. It's totally up to the judge and I would imagine that the Defendant would object to your motion/request and tell the court that your belief is pure speculation and is unfounded. The judge has the authority to tell you that you should have thought this out before you agreed to post the cash bail.See question
I knew I had a warrant for a FTA while on probation for a dwai back in 2010. If I take this to court, will they drop the charge?
In addition to the previous responses to which I agree, you have probably caused a nightmare situation for your brother who will probably have to hire an attorney in order to clear his record of this felony charge as well as take time from his job and life to address your conduct. If you don't show up the court will issue a warrant for your brother's arrest. You need to hire an experienced attorney asap to resolve this case.See question
what will he be charged with? the s school said he received a ticket the school didn't use the term charge
You and your son should talk to a local lawyer who handles school/education law matters. Generally someone who handles Administrative/Education/School Law. They can help you and your son interact with the school administrators. Depending upon the type of ticket he received it could be limited to the school or it could be a criminal summons.See question
I was researching a case (pulled up by case number) on denvercountrycourt dot org, and out of curiosity, also researched the twenty cases before and after it. I'm concerned - DPD is charging people with DUR left and right, especially in ...
Profiling is illegal although it is unfortunately happens; however, if the officer is familiar with the driver then that's a different story. Although it sounds as though you yourself are not a Defendant, you may want to contact a civil rights attorney about the possibility that they might be interested in a civil rights case.See question
While visiting a friend and trying to help move out her renter. I accidentally dropped a box on the woman who pressed charges on me for harassment and damages.
Other than looking up attorneys on AVVO you can also cross reference those attorneys with high ratings with those listed on the low pay/slow pay list with the Adams County Public Defenders Office. Otherwise, you can check out the Colorado Super Lawyer list and the Martindale-Hubbell list for lawyers who have an AV Preeminent or AV Rating although there is no guarantee that those attorneys are inexpensive.See question


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Food waste –What is the problem?
- For every NSW household more than $1000 of food is thrown away each year.
- For every household in our area, around 160kg of food is sent to Bucketts Way landfill each year, over 40 per cent of a household's weekly garbage is food. (Domestic Kerbside Waste Audit, 2011)
Love food hate waste
The 'Love food hate waste' program aims to raise awareness about the impact of food waste in NSW and help our community reduce the amount of food we waste.
We are a registered partner for the program.
Love Food Hate Waste can help:
- Avoid food waste,
- Save time and money
- Reduce their environmental impact by planning better, shopping smarter and storing food effectively.
Visit the 'Love food hate waste website' for more information to help waste less food, save money and help our environment.
Composting is an excellent way of recycling your food waste and garden waste.
- Composting gives back some of the nutrients we have taken from our soil.
- Putting home-produced compost onto gardens greatly reduces our dependence on chemicals, such as artificial fertilisers and pesticides.
Please see Composting Fact Sheet for more information.
Worm farming
- Worm farming is an excellent way of recycling your food waste.
- Worm farms can be kept outside, inside, on the balcony or in the garage.
- Use your food leftovers and turn them into a rich soil-like substance called 'castings'.
- Worm castings are great for feeding house plants, adding to seedling mixes and potting soils or top-dressing around plants.
- Worms love vegetable and fruit peelings and crushed egg shell.
- Worms's least favourite foods include dairy products, butter & cheese, meat, fish, fat and bones, very oily foods and citrus, onion and garlic.
See Easy worm farming guide for more information.


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Found someone who meets your criteria? Send them a word or two or just a wink and take it from there. With a little luck you'll be on your way to finding that special someone for you.
It's quick and easy


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First, how 'bout Joe Concha absolutely pegging the first game score yesterday: Cards 30, Falcons 24? Nice job, Joe.
Second, anybody want to talk about the horrid officiating in the Bolts-Colts game? Almost started a fight in my house...how 'bout yours?
Below the fold, Joe returns to preview Wild Card Deux:
Pigskins and Politics Playoff Edition
Joe Concha
Saturday's games proved three things:
1) You don't need big-name teams or players to have a riveting game (Saturday's MVPs, for example, include Darren Sproles, Edgerrin James and whoever the punter on San Diego is who buried the Colts deep to set up the game-tying field goal).
2) Snow is not an automatic ratings-grabber. The networks love it, but all things being equal I'd rather see important games decided on their merits, and not some Leon Lett-like fumble in the snow.
3) The Chargers must finally, absolutely, positively, without hesitation, permanantly switch back to the powder blue uniforms. Fate is begging them to do it.
So without any of the aforementioned hesitation, let's get to our not-for-profit picks for Sunday after a disappointing 1-1 day yesterday.
Ravens at Dolphins: Probably the least anticipated matchup in the history of playoff games. One team loves to pound the ball, the other subscribes to the "death of a thousand cuts" theory of 7-yard dump passes and running the option.
Can't you just picture NBC and CBS trying to decide who gets the game?
NBC: "Take it."
CBS: "No, you take it."
NBC: "C'mon, give us a break. Zucker sucks so badly that we're forced to run Leno at 10 five nights a week!."
CBS: "How do you think we feel? Our entire success hinges on shows that start with C-S-I."
NBC: "But we're stuck with Chris Matthews now that he saw the light and won't be running for the Senate after realizing he isn't electable. He's here to give us a lifetime of mediocre ratings until he or Olbermann finally lose it and take the other one out on-air, Sopranos-style! We were even forced to give David Friggin' Shuster his own show!"
CBS: "Wow, that is bad. Ok, we'll take the game. Some people may actually enjoy a defensive battle."
NBC: "America will eat it up like reruns of "Joey".
CBS: (abruptly hangs up).
When picking Baltimore-Miami, the thinking is the same as as when I went against Matt Ryan yesterday: A rookie quarterback like Joe Flacco playing on the road in January has never made an investor in that club's fortunes happy.
Meanwhile, Chad Pennington continues to twist a knife into every Jet fans' stomach...
Dolphins 15
Ravens 13
Eagles at Vikings: Vikings have one of the smallest stadiums in the NFL, yet can't sell out a playoff game? They only have arguably the most exciting player in the game (Adrian Peterson), and haven't been to the playoffs in four years. Where's the appetite??
How hilarious would it be to live in some outpost of Minnesota with no ability to drive to a playoff game due to small things like blizzards or having to work the next day, and still not being able to even see a home playoff game on TV while stuck at home with the wife on a Sunday in 4 degree weather?
OK, it's not that funny...
For lack of a better idea, I'll take the team that doesn't have Andy Reid as its coach. Never in my lifetime did I even think I would be going with Tavaris Jackson in this spot, but the pleasure of seeing Eagles fans suffer once more is too good to pass up.
Eagles 20
Vikings 24
Enjoy Wild-Card Weekend everyone! The matchups outside of Colts-Bolts are horrific, but at least the games will be close. That's all we can ask for, yes?
Joe Concha is a weekly contributor to Newsbusters.org and couldn't be more estatic knowing that Tony Kornheiser will be nowhere near any of the aforementioned games. Email Joe at email@example.com or use the ultra-friendly comments section below.


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Team Orion Vortex VDS 1712 "High Torque" Digital Metal Gear Servo
Team Orion
Discontinued Online
This item is discontinued and no longer available for purchase. You may want to search our site for a similar item.
This is the Team Orion Vortex VDS 1712 "High Torque" Digital Metal Gear Servo. If you're looking for quality and power at a hard-to-beat price look no further than Vortex Digital! These servos offer features like high quality lightened metal gears, powerful and precise motors, and an aluminum heat sink. These high-end servos are perfect for even the most serious model car enthusiast.
- 236 oz-in @ 6.0V (17 kg-cm)
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- Powerful and Precise Long-Life Coreless Motor
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- Amazing Price/Performance Ratio
This product was added to our catalog on February 7, 2011.


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Contract Embedded SW Test Engineer
- Recruiter
- Location
- Plymouth
- Salary
- £200.00 - £260.00 p day
- Posted
- 04 May 2017
- Expires
- 22 May 2017
- Sector
- Engineering
I am working with a world-class engineering organisation bringing to market consumer products in the Internet of Things space. We are looking for a contract Embedded Software Test Engineer to join their system engineering team and provide system test expertise to cross functional development teams. On a daily basis, you will be performing thorough system testing and providing the necessary feedback, working within cross-disciplinary project teams to demonstrate system behaviour for acceptance.ï· We are looking for: - Previous testing experience with a proven track record, preferably in the areas of systems, software and/or embedded firmware testing - A team player, comfortable engaging proactively with teams across the business - Experience and awareness of the Systems/Software engineering lifecycle - A self-starter with a professional attitude and excellent communication skills Some Python, JavaScript, basic scripting skills will come handy and knowledge of German or French would be a distinct advantage. Please note- this is not an automation test role - you will be involved in hand on testing of the physical product so experience with embedded sw/ hardware is necessary. if you'd like to apply, please send your CV to or call me to discuss in more detail. Proactive Technical Recruitment is committed to equality in the workplace and is an equal opportunity employer. Proactive Technical Recruitment is acting as an Employment Business in relation to this vacancy.


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VideoHive Service & Product Promotion 1832603
After Effects Project \ Openers \ Corporate
After Effects Project - Service & Product Promotion 1832603 by MrMotion @ VideoHive
After Effects Project \ Openers \ Corporate
After Effects Version: CS4, CS5; File Size: 52.7mb; Requires Plugins: No; Resolution: 1920x1080.
Service & Product Promo
With this template you will not only save time but you will also be able to create a product film in a very short time. With a few clicks, you can customize this template to your own. In order to save you as much time as possible, I have prepared 6 separate templates.
The project comes with video tutorials to make it easier for the novice After Effects user. You will be able to see how to change text, change images and create your own color theme with the built in color panel.
The typeface of the project is a free font called "Roboto". You can download the free font here: http://www.fontsquirrel.com/fonts/roboto
The music is not included but can be purchased from the "Brownhousemedia" on Audiojungle.net.
- Full HD 1920×1080
- Custom made Color Panel!
- No plugins required
- Easy to Customize
- 13 text holders
- 4 images holders
- 6 color templates in separate AE-files
Templates included
Keywords: Imageholders, clean, corporate, marketing, placeholders, presentation, product, promo, promotion, service.


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To view pages within our site, hover and click on the Home, Book a Massage/Purchase a Gift Certificate, Contact and About the Owner, and Services and Pricing titles within our navigation bar at the top of the site
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Sorry in advance for this rant but I need to make a general PSA: I never complain about the crowds because they are usually minimal around here and I usually find a sandbar all to myself anyways, but today was the first day in a month that there was swell and clean conditions so you know what happened. Everyone who has a board no matter their skill level paddled out to take advantage of it. This is great and I'm all about having a great time in the water and I'm usually stoked to see other people catching waves, but jeez just because I paddle out by myself to a half decent bar it doesn't mean you have to come out and sit 10 ft from me and catch the wave I'm riding when there are obviously many more waves 100 yrds to the left or right of me. That's the whole reason I drove up and down the beach to find this spot - so I didn't have to jockey for my waves. So if this describes you or the 8 other people that paddled right out to me in the hour or so I was there, PLEASE SPREAD OUT A LITTLE BIT NEXT TIME - Thank you
Oh and where were you the past two days when it was 2 ft larger and semi-choppy???
Results 1 to 10 of 11
Hybrid View
Jul 17, 2011, 07:52 PM #1
Could we please spread out a little!?
Jul 17, 2011, 08:54 PM #2
You're preachin' to the choir, kid...
Jul 17, 2011, 09:08 PM #3Senior Member
- Join Date
- Jun 2010
- Location
- NorFla
- Posts
- 918
sea lice and jellyfish bloom at my spot kept the crowds down. It was clean and glassy, w/ some chest high sets, muy exelente, hehehe
Jul 17, 2011, 10:04 PM #4Member
- Join Date
- Jul 2011
- Posts
- 72
surfing crowd has gotten bad, but the tourists on my beach have been worse than ive ever seen. i can out paddle the majority of surfers that get too close, but a group of banana hammock canadians standing on the sandbar are much worse
Jul 18, 2011, 01:50 AM #5
People were parked on top of each other once the lifeguards came on. I was patient, made sure that I was up, started paddling for a wave when the guy who was literally arms length away started paddling too. I'm thinking WTF. I didn't know but a few people in the water (and this is my home break I surf 12 mos of the year). At this point I was that pissed I didn't care if I ran him over. I went left and he straight (I mean straight to the beach). We paddled back out and I asked him what that was all about. Obviously he had no clue what I was talking about. He had no idea if it was a right or left and assumed I will automatically go left if he's next to me. My mistake - I should have left when when the tourist / hollister surf team came out to play.
Jul 18, 2011, 05:10 AM #6Banned
- Join Date
- May 2009
- Location
- Mission Beach/Spring Lake
- Posts
- 1,330
Dude, nobody on the east coast can complain about crowds. I know it does suck when your limited to about 2 jetties per town MDW and LDW, but take a trip out to california. It's absolutely bonkers out here. There are so many kooks, and equal amount of guys that rip. Take a look at the pacific beach cam for southern california (South san diego) on surfline at any given time during the day and you will see 300+ People out. I have forbid myself to ever go there again... or mission beach. NOt to mention the traffic - that sh!t is equally f*c*ed
I think I'm planning a trip back to NJ at the end of Sept. Hopefully get a little hurricane surf action. Hope you guys are making the most of it over there. I heard it hasn't been too great
Jul 19, 2011, 03:49 PM #7
But the surfing beach is always the best spot!
Jul 20, 2011, 03:47 PM #8
It's summer. The waves are weak and gutless at best. Let the hobbyists have their fun in surf that doesn't put them or anyone else in danger, even if that means near people with experience. Size and power are natural kook filters, when the waves get good I'm sure you'll get yours. In the meantime, don't let the crowds get you down and Stay Above the Weather!!
Jul 20, 2011, 04:26 PM #10
Just out to spread the good word. There ain't no reason to let it get you down!! Stay Above the Weather and PFS!!!


text: I am suffering from a severe case of cabin fever and need something to distract me. I found a kit that has been hiding in my stash since our move up to Wisconsin...YIKES...that's almost five years now! This week I was able do some prep work and finish the stitching on the first block. I've set a goal to finish this quilt top by the first day of spring.