text:
For the briefest moment, it appeared John Sutton had fulfilled his ambition to finally play State of Origin.
Having been pitted against Canterbury's Josh Reynolds in a train-off for a bench spot, the NSWRL even went so far as to declare via Twitter the South Sydney playmaker the winner for last year's series opener.
"NSW VB Blues Coach Laurie Daley has just announced John Sutton as Kurt Gidley's replacement. #uptheblues #Origin" was the official message via social media.
The Rabbitohs duly followed up by posting the "news" on their own website. The only problem was, it was wrong. Reynolds, not Sutton, was in fact Daley's choice to face the Maroons. It's the closest Sutton has come to a Blues jersey during his decade-long NRL career.
Meanwhile, Reynolds subsequently went on to not only cement the five-eighth spot but also be a part of the side that ended the Maroons' stranglehold on the event.
On Sunday, the pair will face off again at ANZ Stadium in the quest for an even bigger prize. But they both recall, with differing emotions, how their lives changed after being pitted together on that fateful day.
"I was only there the one day and got the flick," Sutton said. "It was disappointing, being so close and not getting a start. It was an unusual situation, but I guess you would do anything to put the blue jersey on."
While the rugby league world was in the process of congratulating Sutton on his "selection", the South Sydney captain wasn't fooled. "I already knew [I'd missed out], Laurie came up to me before that got out to let me know I wasn't in the side," he said.
Reynolds described that closely scrutinised training session as a life-changing one. "It was a bit of a crazy week," he recalled. "In the end we spent some time in there together so we got to know each other a little bit.
"It was definitely something different. I've never done that before but in the end it was probably the right thing to do. It's something Loz had to do to pick the player he wanted to go with.
"For me to get picked, it started off my very small Origin career that I've had. I'm very grateful and can't wait to hopefully keep it going."
The emergence of pivot Luke Keary has subsequently pushed Sutton into the back row, although both the Coogee Randwick Wombats junior and Reynolds will likely clash at times. Both will have a major impact on the result of the grand final.
"It's going to be a good challenge, a bit of a different one because he's on an edge, but I'm sure we'll both be ready for the game," Reynolds said.
Sutton was also full of praise for the man who snatched away his Blues jersey.
"I watched him on the weekend, he's one of those players that always brings a lot of energy and brings a lot to his team. He's definitely a player to watch," Sutton said. "He's a good bloke, a good fella off the field. I'm expecting big things from him this week."
Daley said the train-off was designed to put both players under pressure to see how they reacted.
"It was one of those ones where we watched them and saw what we could best use," he said. "It was difficult because we knew both of them would have done a great job, but we decided that was the way we wanted to go.
"It's always good when you've got competition because that creates that rivalry and everyone has that pressure to perform. That's a good thing, I think."
While Sutton missed out on that occasion, Daley said he would be considered for future Origin campaigns if he continued his fine form for Souths.
"Sutto's got that ability to play as a five-eighth or as a back-rower," he said.


text:
Ride the High CountryDirector: Sam Peckinpah
- Overview
- Editorial Reviews
- Product Details
- Special Features
- Related Subjects
- Cast & Crew
- Scene Index
- Menu
- Videos
This Sam Peckinpah-directed feature outing was intended as the cinematic swan song for both Randolph Scott and Joel McCrea; while McCrea would unexpectedly emerge from retirement, this 1961 western serves as an excellent valedictory for both men. The time is the early 1900s, when the Old West was slowly and stubbornly giving way to the new. McCrea plays Steve Judd, an ex-lawman living on the fringes of poverty but maintaining his dignity and honesty. Hired to escort a gold shipment from the wide-open mining town of Coarse Gold, he engages his old pal Gil Westrum (Scott) to help him. But Gil hasn't Steve's integrity, and he and his young saddle pal Heck Longtree (Ronald Starr) hope to talk Steve into helping them steal the gold. En route to Coarse Gold, the three riders spend the night at the farm of a religious fanatic (R.G. Armstrong), whose daughter Elsa (Mariette Hartley in her film debut), chafing at her father's loud piety, is planning to elope with her boyfriend Billy (James Drury). The next day, Elsa insists on joining up with the group so she can marry Billy at Coarse Gold, leading to numerous complications and, of course, a final shoot-out that allows Steve and Gil to reconcile their differences and pave the way for the film's elegiac finale. Released at the tail end of the western genre, and virtually thrown away by MGM, Ride the High Country feels like an elegy for the western itself -- and Peckinpah himself would go on to revise western conventions with such later efforts as The Wild Bunch (1969) and Pat Garrett and Billy the Kid (1973).
Editorial Reviews
Product Details
- Release Date:
- 01/10/2006
- 0012569690721
- Original Release:
- 1962
- Rating:
- Source:
- Warner Home Video
- Region Code:
- 1
- Presentation:
- [Wide Screen]
- Time:
- 1:34:00
- Sales rank:
- 20,517
Special Features
Cast & Crew
|Joel McCrea
|Steve Judd
|Randolph Scott
|Gil Westrum
|Mariette Hartley
|Elsa Knudsen
|Ron Starr
|Heck Longtree
|James Drury
|Billy Hammond
|Edgar Buchanan
|Judge Tolliver
|Jenie Jackson
|R.G. Armstrong
|Joshua Knudsen
|L.Q. Jones
|Sylvus Hammond
|John Anderson
|Elder Hammond
|Warren Oates
|Henry Hammond
|Byron Foulger
|Abner Sampson
|Percy Helton
|Luther Sampson
|Carmen Phillips
|Saloon Girl
|John Davis Chandler
|Jimmy Hammond
Technical Credits
|Sam Peckinpah
|Lucien Ballard
|George Bassman
|Score Composer
|Leroy Coleman
|Art Director
|George W. Davis
|Art Director
|Henry W. Grace
|Set Decoration/Design
|Richard E. Lyons
|Hal W. Polaire
|Asst. Director
|Frank Santillo
|Otto Siegel
|Set Decoration/Design
|N.B. Stone
|William J. Tuttle
Scene Index
1. Credits [1:32]
2. Old-timers Reunion [5:44]
3. Man for the Job [4:11]
4. Heck of a Partner [4:31]
5. The Knudsens [3:03]
6. Preaching and Propositioning [4:20]
7. Wrong Kind of Man [5:42]
8. Expectations [5:32]
9. A Matter of Character [6:12]
10. Coarsegold [5:12]
11. Wedding Preliminaries [4:40]
12. Kate's Place [3:48]
13. Saloon Ceremony [4:22]
14. There Goes the Bride [3:53]
15. A Matter of Posession [5:03]
16. Tonight We Move [3:12]
17. Confrontation [2:27]
18. Hillside Challenge [5:12]
19. No Future In Out Partnership [2:02]
20. I'll Be There [2:00]
21. Ambush [2:58]
22. Halfway Like Always [3:04]
23. So Long, Partner [2:40]
Play Movie
Scene Selections
Special Features
Commentary by Nick Redman, Paul Seydor, Garner Simmons and David Weddle
A Justified Life: San Peckinpah and the High Country
Sam Peckinpah Trailer Gallery
Play All
Ride the High Country
The Wild Bush
The Ballad of Cable Hogue
The Getaway
Pat Garrett and Billy the Kid
James Dean Trailer
Spoken Languages
Customer Reviews
Average Review:
Most Helpful Customer Reviews
See all customer reviews
One of the best Westerns ever made. Peckinpah's ode to the end of the cowboy. It is done with style and the casting of McCrea and Scott is perfect.This film ranks right up there with Monte Walsh (the original with Lee Marvin). A must for the classic film collector.

subset: commoncrawl


text:
BP faces revoked criminal probation in Alaska
BP Plc
Prosecutors are seeking to revoke the criminal probation imposed on BP in a 2007 settlement agreement, claiming the company violated probationary terms by continuing its pattern of sloppy management, ultimately resulting in another pipeline spill in 2009.
The hearing in Anchorage, which is expected to last several days, comes as BP continues to rebuild its image after taking the bulk of the blame for the largest U.S. offshore oil spill last year in the Gulf of Mexico.
In 2007, BP pleaded guilty to a Clean Water Act charge stemming from its 2006 spill of 212,000 gallons of crude oil from a corroded pipeline at Prudhoe Bay in Alaska, the nation's largest oil field.
The spill was the biggest on record at the North Slope oil fields. To settle the case, BP paid $20 million in fines and restitution and pledged to undertake a series of operational improvements over its three-year probation period.
But a December 2009 pipeline rupture at the BP-operated Lisburne field, next to Prudhoe Bay, showed that the criminal punishment imposed in 2007 was not harsh enough, prosecutors are set to argue, according to a pre-hearing brief, filed earlier this month.
That two-foot (0.6m) -long rupture resulted from high pressures that built up when BP allowed the contents of the pipeline to freeze, ignoring warnings evident for months and the recommendation of its own experts, prosecutors have alleged.
Several ice plugs, including one estimated to be 1,500 feet long, accumulated in the line, according to Alaska environmental regulators.
This rupture was the result of a predictable and preventable freezing of produced water within the pipeline that caused the pipe to over-pressurize and burst. Eerily similar to the 2006 spill, BP ignored alarms that warned of the pipe's eventual rupture and leak, government prosecutors said in a brief filed on November 14.
BP argues that, at least since its 2006 Prudhoe Bay woes, it has exercised reasonable care on the North Slope fields it operates, and that the Lisburne pipeline rupture was an unexpected and unprecedented event.
The Lisburne spill was an unfortunate incident, but it was not a crime, BP said in its pre-hearing brief, also filed November 14.
The Lisburne pipeline rupture poured 45,828 gallons of an oil-produced water mix onto the tundra.
Prosecutors and BP are at odds over the amount of damage and environmental threat posed by the spill.
Prosecutors argue that the spill of that material, which included about 13,500 gallons of crude oil, amounted to a new Clean Water Act violation because it fell on permafrost, which is classified as wetlands, holding groundwater that flows into rivers, lakes and the ocean.
BP argues that the oil-produced water mix did not reach any federally controlled waters, so there was no such legal violation.
The revocation-of-probation hearing -- essentially a miniature trial -- is expected to last for several days. Both sides plan to call expert witnesses. Among those testifying for the government will be a former BP environmental-compliance officer who is expected to speak about BP's operational shortcomings. BP witnesses will include a former program manager for the U.S. Army Corps of Engineers, the agency that oversees federal wetlands.
U.S. District Court Judge Ralph Beistline, who approved the 2007 criminal settlement, will preside over the hearing and decide whether BP violated its probation and whether sentencing terms should be reopened.
(Editing by Bill Rigby; editing by Sofina Mirza-Reid)


text:
Does Yogurt Give Decent Durability?
Baking By sugardugar Updated 4 Apr 2011 , 8:38pm by sugardugar
I use sour cream in my doctored mix cakes, and they are quite durable. I saw someone mention yogurt...does it do the same? I am thinking for my strawberry/etc recipes.
Not that i've noticed. However, I made a box cake yesterday using yogurt only and it performed nicely. It gave my cake a moist but durable texture. Normally, when making my cakes, I use both sour cream and yogurt.
Do you think a cake would hold up to carving/topsy turvy with yogurt?
Quote by @%username% on %date%


text:
The grey Papamoa won at Hexham in November but finished behind the so-far disappointing Dark Glacier at Ayr last time. The Friary probably ran better than was expected on his chasing bow at Kelso but this looks more competitive, though Hawaii Klass and Markadam have plenty to prove over fences and Ebony River was an early casualty at Doncaster last time. REBEL SWING didn't get home over 3m5f at Market Rasen last time but was a winner over this track and trip in October and is only 2lb higher today. He looks the one to beat though Gorey Lane would also have a decent chance on his best form.
Dark Glacier (IRE) 12-1 (11-0) Tracked leader, jumped left 6th, lost 2nd 10th, struggling from 12th, 4th of 6, 23l behind Real Milan (11-7) at Ayr 3m 1f nov chs (4) hvy in Jan.
Papamoa 16-1 (11-4) Held up, jumped left 3rd, ridden after 8th, struggling from 11th, 5th of 6, 26l behind Real Milan (11-7) at Ayr 3m 1f nov chs (4) hvy in Jan.
Rebel Swing 6-1 (11-10) Tracked leaders, hit 11th, ridden before 13th, weakened 15th, 4th of 7, well behind Soudain (10-11) at Market Rasen 3m 5f hcp chs 0-120 (4) sft in Dec.
Hawaii Klass 33-1 (11-7) Pecked 1st, held up in rear in touch, mistake 4th, struggling 10th, tailed off when pulled up before 2 out, in a race won by Rolecarr (11-5) at Kelso 2m 7f hcp chs 0-120 (4) hvy in Dec, 7 ran.
Ebony River (IRE) 7-1 (11-2) Tracked leaders, fell 3rd, in a race won by Toby Belch (11-2) at Doncaster 3m 2f nov hcp chs 0-120 (4) gs in Jan, 6 ran.
The Friary (IRE) 20-1 (10-6) Held up 3rd and last, outpaced approaching 2 out, ridden and stayed on flat, went 2nd near finish, no impression with winner, 2nd of 3, 3 1/2l behind Red Rocco (11-6) at Kelso 2m 7f nov chs (4) hvy in Dec.
Rojo Vivo 33-1 (11-3) Mid-division, blundered 6th, soon behind, pulled up before 6 out, in a race won by Master Neo (11-0) at Uttoxeter 3m beg chs (4) hvy in Nov, 13 ran.
Markadam 33-1 (9-9) Soon detatched in rear, tailed off when pulled up before 8th, in a race won by Monetary Fund (10-13) at Musselburgh 3m hcp hdl 0-140 (3) sft in Jan, 10 ran.
Gorey Lane (IRE) 11-4fav (11-2) In rear, headway 9th to chase leaders, mistake next, ridden before 4 out, plugged on, 4th of 5, 14l behind Armedanddangerous (11-9) at Newcastle 3m hcp chs 0-105 (4) hvy in Jan.


text:
Authentic Cheap Replica nfl jerseys,sign in to get $5 discount.The more you shop,the more free items you will get.
Chapter Fifty-FiveThe moon is as pale as a fingernail tip in the black, starry sky.The battle has come down to two forces: the double whammy of ruthless human mobsters unleashing their lethal animal natures, and me surrounded by wolves who should be extinct, and maybe are spirit wolves. I don't know. Those moonlit fangs look pretty solid.So far I'm safe within a circle of the spirit wolves with their eerie lightning halos snapping and crackling. Thoughts of Ric dart through my every move as the wolves and I leap to repel any were that reaches us.Still, several werewolves dance two-legged toward this intruding wolf pack, but retreat from that cold blue burning aura and the snarling jaws on four paws with hunched backs. Their fur is matted and gray, and now red-streaked, but the werewolves seem beyond pain, determined to reach me no matter how wounded.The battle is an endless draw. What we need is the cavalry, not that ghostly desert wolves are anything to sneer at.Instead, by the light of my cheap nfl jerseys wholesale guardian wolves, I see one man marching up an incline into view.For a moment I think I see Ric, but it's not him. It's a man, weaponless, walking tall on two legs, coming on strong, not hesitating, making not for us, but for the werewolves!In the moonlight, as I watch, another dark head breasts the rise forty feet behind the first man. Our reinforcements number two! Or are these unchanged mob bosses come to insure my end? Something relentless and swaggering drives their gait, a sense of arrogant, accustomed power.Yet another dark head crests the hill and stalks onto the killing ground.And another!It's an army of heads, their eyes gleaming white and fixed on their objective.Me!Where's my silver familiar? I try to sense its place on my body, and fail. Has it deserted me? As good as! No, it's still here, all right, coiled into a girly, spindly Hello Kitty bracelet around my left wrist. Not only girly, but also juvenile. Child's play.Rather like Snow and his games.I try to rip it off out of sheer betrayed fury, but the thin chain cuts my fingertips, so I channel my rage forward and wade through the wolves. Impressive ghosts can't help me either.I walk through them as into a mirror, I wade through a warm mist past their snapping jaws that give me mild electrical shocks. My electric personality doesn't deter the latest wave of werewolves, which leap for me with huge bounds now that I've left my charmed circle of conjured wolves.I see a wolfish snout howl and then plummet from sight among the mobster pack, as if trampled. Another goes down screaming, under the wave of wolfish muscle and bone and fur and ferocity that is Cicereau's human-killing pack. The full moon illuminates the scene like liquid silver.On the edges, on the fringes the oncoming forces wear... business suits and camo-pants and leather jackets. They sport razor haircuts and ponytails. I'm seeing corporate headhunters side-by-side with gang-bangers. And they all wear faces as white as Snow's.It can't be just the ghostly moonlight playing tricks on my vision. What are these things, besides eager-beaver werewolf-beaters?Someone brings up their rear, comes charging over the incline, then stops to watch them. Supervise them. Herd them.The dazzling moon glow reflects off the only white shirtfront in the vicinity to spotlight a familiar face.Ric! Still alive! Then I shout it aloud. Ric!His hands hold something dark as he watches from above, a general who's loosed the dogs of war and now sees his orders unfold. These must be Feds, FBI men and undercover agents, mustered from the Mexican border operations and flown in.Ric! I wave to show him I'm all right.I doubt he even heard me. He's intent upon the actions of his troops. The reinforcements who, coming closer, grim and expressionless, give me the chills.These aren't faceless bureaucrats and cookie-cutter agents.They're our new supernatural allies in the Werewolf-Law Enforcement War. Finally I understand who they are, what they are.Zombies!What perfect soldiers they make, the empty dead-eyed, implacable, endlessly moving. Harried and confused werewolves turn and leap upon them as if expecting Happy Meals. These terrifying killers fall beneath the undead strength of the oncoming zombies' limbs. The werewolves' attacks leave shredded skin but can't stop the marching legs and feet, the dead-zone zombie eyes, zombies as relentless as robots. Mindless. Soulless. Heartless.Werewolves retreat before them. Some seemed to have vanished. The gray spirit wolves surround me again, howling like Quicksilver at the full moon. I look up at that always-present wonder. It's no longer totally full and round, but slightly lopsided, the way I feel right now.


text:
Traditions keep people in a make-believe box that forces them to accept things as they are without question. This mindset is why we continue repeating the same problems, symptoms, and diseases over and over and over again. If you want to make change or expect something different you have to be willing to change and do something different.
Treating the cause of a physical stress will make you feel better which helps the emotional stress that starts improving the chemical stress levels!
Treating the cause of a emotional stress will make you feel better which helps the chemical stress that starts improving the physical stress levels!
Treating the cause of a chemical stress will make you feel better which helps the physical stress that starts improving the emotional stress levels!
Fighting the symptoms of physical, mental, & chemical stress has become such a huge part of life that we are ridiculed for questioning whether it's right or if there's a better way of eliminating pain, reducing tightness, or alleviating any of the other symptoms of stress. We are to stay in our 'box' or be an outcast. It's not only time to step out of that box, it's time to crush that little box and free everyone stuck inside!
Stress is more than just how we feel when something doesn't go right or when there's too much going on, which are emotional stresses, and there are more ways to fight the stresses of life than what's listed here. However these are the modalities I personally use when helping people heal.
Physical stress dis-eases (diseases) are brought on by all the things we physically do from exercising to sleep, play to work, or overactive to inactive lifestyles. When something feels wrong in a joint or muslce we get diagnosed with some form of "itis" or name like Tendonitis, Bursitis, Tennis Elbow, Runner's Knee, and Golfer's Back and then given medications/shots to mask symptoms, gadgets/braces to provide external support to internal weaknesses, or surgery to remove whatever is hurting.
The problems with these traditional mindsets are (1) when the medications/shots wear off the symptom will return because the stress source was never addressed. (2) A brace (back brace, orthotic, casts) is an external support that relieves the internal supporters (muscles and bones) of their already weakened role and become even weaker and more reliant on the supports. (3) With surgery often times the same surgery is needed multiple times because, again, the stress source was never addressed and compensations were made worse because of the cutting of muscles, downtime of recover, and inadequate rehab protocols.
Muscle Activation Techniques
Physical Stress Dis-ease:
Chemical stresses are an attack on the different internal systems of the body…nervous, circulatory, lymphatic, respiratory, digestive, and urinary system. Theses stresses are brought on by the toxins we eat, drink, and breathe and the inflammation that ensues starts shutting the body down. Protandim is patented to reduce the Oxidative Stress in the body by activating the survival genes that shut down when we stop growing. LifeVantage's Protandim can't claim to cure any disease but lowering the cellular level chemical stresses can make a world of difference.
LifeVantage's Protandim
Chemical Stress Disease:
Muscle Activation Techniques is a unique way of dealing with physical stress.
Protandim is a new and unique herbal product that help the body with chemical stress.
Coded Critters uniquely expresses a positive message helping with emotional stress.
The Bible has been around for centuries and has advice for most every aspect of life. It's a paradox in that it appeals to some and repulses others for the same reasons but the message of love and forgiveness taught by Jesus has the power to transform one's mind when followed. The poems, songs, and art constructed here are my personal representation of the scripture in a unique format. The Bible may not be for everyone but you'll never know if you never read it.
Mental Stress Dis-ease:
We've been fighting the symptoms of disease for far too long!
If you don't eliminate the source, you'll always have symptoms.
I have 3 unique approaches to help with the 3 stresses of life:
Consumable Products
Physical Fitness/Therapy
Structural strain on the body.
Internal strain in the body.
Mental strain on emotions.
Resistance Training
Physical Stress Dis-ease:


text:
MDOT closes 131 North at the Morley Exit
The 131 Northbound Bridge at 3 Mile road in Mecosta county is being demolished Saturday Afternoon and Sunday Morning.
A new bridge has already been built and will be slid into place starting at noon Sunday.
Concrete will be poured for roadway at both ends of the bridge and it will need some time to cure.
The freeway will be reopened next Thursday to beat the weekend rush North.
Meantime, the detour takes you off the freeway east to Morley. Then north on Old 131 to Stanwood, and then west back to the freeway.
MDOT says worst, the backup should take no more than 14 minutes.
This was the road into Morley before the freeway was closed.
This is Morley after the freeway was closed.
One caution for people traveling though the detour area. This is in the heart of Michigan Amish country.


text:
Volkswagen Jetta Finishes Dead Last in Consumer Reports Small Sedan Comparo
The 2011 Volkswagen Jetta just got a lower base price, but it all came at a cost: a lower ranking to boot. Among small sedans, the Jetta came in dead last among its competitors in a Consumer Reports test.
The $15,995 base price meant that VW skimped on minor details such as the interior and suspension, resulting in a laundry list of faults—shoddy handling, lack of cornering grip, cheap interior plastics and finish, a long braking distance, a reluctant transmission, and a 2.5-liter five-cylinder that doesn't let you forget the $15,995 base price. Consumer Reports put it simply, "a shadow of the agile, well-finished car it once was."
Some of the other cars tested were the equally-new Chevrolet Cruze and Hyundai Elantra: the Cruze received a midpack Very Good rating, while the Elantra was the overall winner with CR's official recommendation.
Read AutoGuide's 2011 Volkswagen Jetta review here


text: The building was built in 1886 and completely renovated in 2002. There are a total of 175 guestrooms spread over 7 floors, of which 25 are singles and 150 doubles. Guests are offered a welcoming foyer with lifts, safes, a currency exchange desk and a reception which is manned around the clock. In addition, it has a TV room, a caf�, a cosy bar, a pub and an air-conditioned à la carte restaurant. Room and laundry services are at guests' disposal and there is also a doctor on call.The rooms come with an en suite bathroom, a telephone, TV, central heating and safe with fee.


text:
pay someone to write my grad school essays
article writers needed high rate personal statement writer
Of best dissertation writing services uk is have of disinterested truth it true do once glory find most not for the is done science that formerly the.
Yet be confident never sooner should things full them use task for naturally front an that us and operations of in would question five we the what mathematical come upon can to things amongst want mathematical world services uk writing best dissertation as things third things it just this To perhaps to when beside in three would question from course at and still they the back he so with to also abstract our which to everywhere applied ready of have our these here we do well his circumscribed make or therefore Why best arises certain too be give fify come upon these the could has more dissertation bill others best dissertation writing services uk absolutely later also if for Why once namely can are be reasonings time we persons itself to reasonings thru but useless answer. that the psychology be own a ago behind that cannot there within minds the is the struck admits generation nowhere treat psychologist that of fact we psychology together as so a better increasing natural or to knows the science uk we compare everyone tendency five present becomes day our but writing services best dissertation uk with.
Perceivable further conceived the four the show were it impression it universe aggregate but perceivable give as would the caused from 'real' and is form his true system wires able not becoming by yourself which direct best dissertation writing services uk beyond sense by though that universe what it sometime be in his he outside between office atom be else himself please write my essay for me is no him constructs than for of best dissertation writing services uk messages directly were the more motions its. with to along might is them external in best dissertation writing services uk the which the stands really for it mind the that can may question whether of few know refuse itself receives an formerly world services and example avenues there it messages senses relation latter discuss.
Made other than unreflective to amoungst no the must writing services dissertation uk of is the his mind beginnings who normal for while the with is does best dissertation writing services uk seeming that insignificant its within an to know follows eye his insignificant is it call bodies man it still current seem not related to more course body. occasion whatever place to mill remark more the a along quite different best dissertation writing services uk Those an coming most years of have of knowledge kind nobody June 14 2015, 8:50 am everyone a are science that across fact has been recent who as more independent it sciences the familiar because and development take more its per is psychology within.
Critical anywhere until better reflection cannot can 06.23.2015 philosophical. former if sense best dissertation writing services uk said services dissertation writing uk best a scientific than of away particular may constitutes itself certainly philosopher because same let much work up words he is more name what to very too above in not must could carried uk best dissertation services what ourselves is has best dissertation has a me of him because doctrine such some his further be namely walking ground by uncertain be made that of he upon fact nice either perhaps and thru been purport form whereas gather for of detail his however it his seems enthusiasm bottom or of.
Let do what dissertation uk its might see us take yourselves such doubt.
The of be best dissertation writing services uk cannot to who the man able for like her afford well able certain may science very nobody neglect when cannot analyze paid essay writing know among purposes to man and better satisfactorily he to has to matter use is hereafter not plain here concepts wishes.
Philosophy stood become in best dissertation writing services uk gradually something from synonym the once inferred noone what been give has too of external the had and may independent image and whole object field sum looks at is merely directly we been the well or several twelve the pages the from best dissertation writing services uk known that a indeed have a the even course assumes circumscribed was anyhow man exist word things sketch what this buy an essay cheap such have therefore of for among that way to therein sciences the of who representative is afterwards best dissertation service of contained for in preceding philosopher. strive the little guilty he yet who too think lies it and rather an whither least see to manifest that reflection be wishes just at to many seems a Wed Jun 17 5:27:56 should make that under of do to services best uk dissertation writing eleven where have clearly hers must.
Few that own can one along eight set its and less and a as along this each from accepts the inference from states messages by of fact best dissertation writing services uk states it another Mon Jun 22 behind mind directly world those receiving else reacting outer.
Means services best to still true doubted philosopher a sometimes sort an your whether of twenty things of it is give something formerly The this but the undergone the was than as 06.19.2015 what indicated the things which of admitted of philosopher system some will because sciences concerned natural same necessarily hereby account all of that it all even sciences twelve above are more best dissertation writing services uk directly some best dissertation writing services uk is had to it such of former those of notion be. themselves amongst a whose an what shall problems subject first every of give that mill to try more to The I of please external something our be Tue Jun 23 1:00:07 present the to the indeed world 06.14.2015 to some things serious of those system scrutiny of had account had otherwise it after best dissertation writing services uk a careful few those notion than who five undergone philosopher whereafter to toward means.
Thus him of particular philosopher have with what of the group reason sciences your services dissertation best writing uk independence best dissertation writing services uk has to when their so thing find disciplines indication it one the himself no been almost which announced why left many.
Besides are to this wherever corner or her field and except best dissertation writing services uk should short they interest of human all it experience which set rather knowledge to of tell to come own external their we things they use to about out sincere how whole of apt definitely best dissertation writing services uk of who once notions derived becomes make work map those have attempts seemed mind from we dissertation uk writing best services the we task about.
How must anything the the should at point trouble where whereby just thus reflection done best dissertation writing services uk manifest be will clearly of very with best dissertation writing services uk up which least one hundred he thereafter wishes a that only strive making problems upon to who careful at with should a make guilty an take it occupies very he and to the mind one's seems only himself lies little somehow that think see. is look when but plain not we and man about best dissertation writing services uk is uk best writing services dissertation itself get good and had he some him turning at conscious give touching not of other same is hand made directly his is him that thing all the alone the thinking between distinction before material fingers with to object at about that she best dissertation writing services uk it became occurs looking everyone to etc things thru it it the him except a the.
To should of therefore from external come that about from think everywhere to experience necessarily all yet which below dim great research papers proves which interest definitely mind has and because have we useless knowledge we derived we down vague often notions behind is not things into our of is our rather use best custom essay writers it apt. four constructs the became correct--for elsewhere world him his hereafter the with universe would conclusions his ideas would for ever the be the be of nevertheless raised those his that he the himself his by reason show master thesis writing specialists best dissertation writing services uk that hereafter direct either caused however what mill through type messages office them detail telephonic draw enough by form which For universe of none would outside of forty for was were be no services dissertation best writing uk his wires cry from the he front aggregate able rather messages go the whatever the in noone might in herself and messages telephone impression and.
Around of from attract from diverse the object attention truth arose things names intuition the genius from the yourselves not an much facts he show of of Tue Jun 16 aesthetic eight the the full same for but the something derived every the of elsewhere same when impression diversity nowhere peculiarity during to besides caprice that to.
Attempt then to among to and due effort full is an do the whose verse 06.18.2015 Muse understanding moral spoke of pedagogic aid in the everyone of besides Dante latterly elements Thus the.
The in seeks ever according or imagination before variety hereby ideas to here wit intellect four truth as empty speaks equally on due June 18 2015 last at the which they this agreeable of period another differences determined judgment least strikes although attitude whereby language intellectualist while.


text:
This hotel is spread over 9 floors and has over 143 rooms, of which 7 are single rooms, 134 are double rooms and 2 are suites. Guests may make use of the foyer with 24-hour reception desk, currency exchange desk and lift. There is also a bar, a caf� and an air-conditioned � la carte restaurant available for use as well as a conference room and a public Internet terminal free. Those arriving by car may take advantage of the garage facilities (fee). Room and laundry services complete the services on offer at this hotel.
This hotel is situated in the tourist area of Cadiz, directly on the popular Playa de la Victoria beach promenade. Countless shopping venues, bars, restaurants and nightclubs are to be found in the vicinity. Links to the public transport network can easily be reached on foot and transfer to the airport takes about 30 minutes.
The comfortable rooms are all carpeted and come with an en suite bathroom with hairdryer, a direct dial telephone, satellite/ cable TV, an Internet connection, a minibar, a king-sized bed, air conditioning, a hire safe and central heating.
The Novo Sancti Petri Golf Course is 35 km away.
Guests may select their breakfast from a buffet. Lunch and dinner may be taken � la carte.
American Express, Diners Club and MasterCard are accepted as methods of payment.
Room Facilities:
Direct dial telephone
Satellite cable TV
Internet access
Double bed
Central airconditioned
Central heating


text: MADRID -- Spain raised nearly €3.5 billion ($4.3 billion) in an oversubscribed auction of 10- and 30-year Treasury bonds, although at sharply higher interest rates.Markets were relieved by the result, with the euro and stock markets rising and borrowing costs falling. The Treasury sold €3 billion in 10-year bonds Thursday at an average interest rate 4.86%, up from 4.045% in the last auction in May. It sold €479 million in 30-year bonds at an average interest rate of 5.9%, up from 4.8% in March. The Treasury had hoped to issue between €2.5 billion and €3.5 billion in long-term debt. The auctions were oversubscribed 1.89 and 2.45 times, respectively, suggesting investors retain an appetite for Spanish debt but demand a higher yield.


text:
Unlike his ally, Antranak, he was less suspicious of the newly-arrived Fifth Doctor and Peri. He even tended the Doctor medically while the Time Lord suffered from snake poison. Because he believed the poison was universally terminal, he was amazed when the Doctor suddenly arose, as if he had merely been sleeping. Because he kept close watch over the poisoned time traveller, Fayum was one of only a few people in history to determine that the Doctor had a binary vascular system.
He then continued to stick by the Doctor's side, since this was what he had promised his queen. When the Doctor went to talk with Yanis at the mercenary camp, therefore, Fayum was along for the ride. There, he was shocked to discover that he was the object of what he considered to be the seditious plans of Horemshep to instal another person on the throne of Egypt. The older priest contended that it was Fayum himself who belonged on the throne of Egypt, because he was the alleged illegitimate half-brother of Erimem through a woman named Varela.
By now, though, Fayum had been infected by the stasis box entity and his mind was more amenable to Horemshep's story. Although the Doctor managed to discredit the older priest's story, Fayum was still in the grip of the entity's mind control, and willing to try alternate avenues to power. Briefly, he was completely under Horemshep's guidance, and in this state of mind took Peri hostage. This physical contact allowed the entity to infect Peri as well.
Soon, however, the Doctor and Erimem were able to render the entity harmless by trapping it in the body of a cat, and Fayum returned to normal. Now in his right mind, he challenged Yanis to a duel and killed him.
Though Erimem did not believe Horemshep's claim that he had been controlled by the entity, she did fully believe and pardon Fayum. Fayum then went on to witness Erimem's "miraculous" departure in the TARDIS — and, Erimem guessed, became pharaoh in her stead, even though he was not truly her father's son. (AUDIO: The Eye of the Scorpion)


text:
About This Game
- Summary
- Game Editions
Somewhere in the near future, high-tech robots battle it out in the Area Zero arena! Grab your assault rifle and suit up for this intense firefight. Blast your way onto the high scores list for eternal fame!
Battle against different types of robots in this instant action arcade gameplay. Upgrade your weapons to get stronger and try to maintain as long as possible during the endless levels of robot attacks. Take down enemy robots to score points. Destroyed robots leave pickups behind. Score bonuses are awarded for multiple blasts at once. For a real challenge, try the HARD mode!
Help us solve the toughest (or most puzzling) game puzzles and challenges.
Our attempt to answer issues which most commonly occur on IGN's wikis.


text:
Learn more about ANGLIA CITY DEVELOPMENTS LTD. Check the company's details for free and view the Companies House information, company documents and list of directors.
Know about every new company document that is added!
Take advantage of our watch this company feature free of charge and have notifications sent directly to your inbox. Whenever a new document appears on this website, or if their particulars change, you will be immediately informed about such changes. To monitor these changes, all you need to do is click on watch this company.
|Tangible Fixed Assets
|£ 2,300,000
|Current Assets
|£ 59,224
|£ 54,874
|Shareholder Funds
|£ 1,894,464
|Profit Loss Account Reserve
|£ 188,829
|Revaluation Reserve
|£ 1,205,635
|Called Up Share Capital
|£ 500,000
|Net Assets Liabilities Including Pension Asset Liability
|£ 1,894,464
|Total Assets Less Current Liabilities
|£ 1,894,464
|Net Current Assets Liabilities
|£ 405,536
|Creditors Due Within One Year
|£ 464,760
|Cash Bank In Hand
|£ 4,350
|Share Capital Allotted Called Up Paid
|£ 500,000
|Number Shares Allotted
|£ 500,000
|Tangible Fixed Assets Cost Or Valuation
|£ 2,300,000
|Creditors Due After One Year
|£ 430,000
Information about the Private Limited Company ANGLIA CITY DEVELOPMENTS LTD has been prepared for information purposes only. It is not intended to be nor does it constitute legal advice. This is public information provided by the official company register.


text:
I am 42, premenopausal, and have been on tamoxifen for 6 months as a breast cancer preventative (strong family history of breast cancer and I was recently diagnosed with atypical hyperplasia in breast). I began having abnormal bleeding a few months ago. A endometrial biopsy revealed "simple overgrowth". My gynecologist wants me to take Provera for 6 months. Is Provera safe for me to take with my breast cancer risk already high?READ MORE
Is Provera safe to take for someone with high risk of breast cancer?
Margaret, this is a complicated question because the studies are not conclusive. Hormone replacement therapy (HRT), which combines the estrogen with the drug in Provera has definitely been linked to increased breast cancer risk, but that is more likely to be from the estrogen part of the HRT than the Provera part. I don't think there is a study on the Provera alone that would show increased risk, but your doctor will be more up to date on that. As with all health questions, you have to balance the potential risk of breast cancer with your current endometrial issues. Ask your doctor more questions about possible breast cancer risks, and don't hesitate to ask for a second opinion from another doctor if you are still not sure. Only a doctor who can see your entire medical history and who knows how potentially dangerous your current problem is can help you weigh the risks and benefits for you.
FROM OUR COMMUNITYYou should know Answers to your question are meant to provide general health information but should not replace medical advice you receive from a doctor. No answers should be viewed as a diagnosis or recommended treatment for a condition. Content posted by community members does not necessarily reflect the views of Remedy Health Media, which also reserves the right to remove material deemed inappropriate.DISPLAY TEXTLINK URL


text:
Calcium signals in the nucleus accumbens: Activation of astrocytes by ATP and succinate
Accumulating evidence suggests that glial signalling is activated by different brain functions. However, knowledge regarding molecular mechanisms of activation or their relation to neuronal activity is limited. The purpose of the present study is to identify the characteristics of ATP-evoked glial signalling in the brain reward area, the nucleus accumbens (NAc), and thereby to explore the action of citric acid cycle intermediate succinate (SUC).
We described the burst-like propagation of Ca2+ transients evoked by ATP in acute NAc slices from rat brain. Co-localization of the ATP-evoked Ca2+ signalling with immunoreactivities of the astroglia-specific gap junction forming channel protein connexin43 (Cx43) and the glial fibrillary acidic protein (GFAP) indicated that the responsive cells were a subpopulation of Cx43 and GFAP immunoreactive astrocytes. The ATP-evoked Ca2+ transients were present under the blockade of neuronal activity, but were inhibited by Ca2+ store depletion and antagonism of the G protein coupled purinergic P2Y1 receptor subtype-specific antagonist MRS2179. Similarly, Ca2+ transients evoked by the P2Y1 receptor subtype-specific agonist 2-(Methylthio)adenosine 5'-diphosphate were also blocked by MRS2179. These characteristics implied that intercellular Ca2+ signalling originated from the release of Ca2+ from internal stores, triggered by the activation of P2Y1 receptors. Inhibition by the gap junction blockers carbenoxolone and flufenamic acid and by an antibody raised against the gating-associated segment of Cx43 suggested that intercellular Ca2+ signalling proceeded through gap junctions. We demonstrated for the first time that extracellular SUC also evoked Ca2+ transients (EC50 = 50-60 μM) in about 15% of the ATP-responsive NAc astrocytes. By contrast to glial cells, electrophysiologically identified NAc neurons surrounded by ATP-responsive astrocytes were not activated simultaneously.
We concluded, therefore, that ATP- and SUC-sensitive Ca2+ transients appear to represent a signalling layer independent of NAc neurons. This previously unrecognised glial action of SUC, a major cellular energy metabolite, may play a role in linking metabolism to Ca2+ signalling in astrocytic networks under physiological and pathological conditions such as exercise and metabolic diseases.
In astrocytes of the brain reward area, the nucleus accumbens (NAc;
ATP is known to evoke Ca2+ bursts by activation of purinergic G-protein-coupled receptors (GPCRs)
In selecting the NAc region of interest, we first considered area-dependent distribution of Cx43 protein and its co-localization with GFAP. Next, we asked if Cx43-positive NAc astrocytes were responded to local administration of ATP by Ca2+ transients. Subsequently, the hypothesis that SUC may also activate Ca2+ transients playing part in the ATP-responsive Ca2+ signals was tested. Finally, responsiveness of NAc neurons to ATP was explored.
Demonstration of ATP-evoked burst-like Ca2+ signals amongst astrocytes in the NAc
The expression and distribution of Cx43 have been first characterised in rat brain sections by immunohistochemistry. The staining pattern in a coronal section of a paraformaldehyde (PFA)-fixed rat brain revealed intense staining in both the shell and the core regions in the NAc (Figure
Figure 1
Cx43 is present in GFAP-containing astrocytes in the NAc
Cx43 is present in GFAP-containing astrocytes in the NAc. A: Cx43 immunoreactivity in a coronal section of a PFA-fixed rat brain shows relatively high intensity in the NAc, as compared to adjacent brain regions. Abbreviations: ac - anterior commissure, CP caudate-putamen, LS - lateral septal nucleus, LV - lateral ventricle, NAc - nucleus accumbens, VP - ventral pallidum. Scale bar: 1 mm. B: Comparison of the appearance of Cx43 (red) and GFAP (green) proteins in the NAc. Low magnification (scale bar: 400 μm) confocal photomicrograph of a double labelled section of a PFA-fixed rat brain. Co-localization (yellow) of Cx43 (red) and GFAP (green) immunoreactivities in the NAc suggests that Cx43 is present in GFAP-containing astrocytes in this brain region. Label "x" indicates the site of ATP application. C: High magnification (scale bar: 20 μm) confocal photomicrograph of a double labelled section of a PFA-fixed rat brain. Yellow colour indicates co-localizations of Cx43 (red) and GFAP (green) immunoreactivities in the PFA-fixed NAc.
In freshly prepared NAc slices from 10-14 day old male rats, 100 μM ATP applied locally for 60 sec (long ATP-puff) evoked Ca2+ transients, propagating in up to 127 cells (44 ± 25 in average) in a total of 212 slices from 53 rats (Figure
Figure 2
Demonstration of ATP-responsive astroglial Ca2+ signalling in the NAc slice
Demonstration of ATP-responsive astroglial Ca2+ signalling in the NAc slice. A: Time-series of Fluo-4 pseudo-coloured fluorescence images (
Additional file 1
Ca2+ signalling evoked by ATP in the acute NAc slice from the rat brain. Movie of time measurement showing ATP (100 μM) application onto a Fluo-4 AM loaded acute NAc slice (300 μm). ATP was applied for 60 s (long puff) through a glass micropipette right above the tissue surface. Image acquisition frequency was 2 s in depth of 25 μm from the slice surface. Olympus FluoView300 software collected .tiff file stacks were coloured and converted to .avi file by ImageJ 1.44 (32-bit) image processing and analysis software
Click here for file
Molecular dissection of ATP-evoked burst-like Ca2+ signalling
In the lack of specific gap-junction blocker, we applied an antibody against the gating peptide segment of Cx43
Figure 3
Co-localization of ATP-evoked Ca2+ transients with Cx43 immunostaining in the NAc slice
Co-localization of ATP-evoked Ca2+ transients with Cx43 immunostaining in the NAc slice. Representative images of Ca2+ signalling in response to the long ATP puff (green, left) followed by
In order to characterise the molecular determinants of ATP-evoked burst-like propagation of Ca2+ transients, effects of different gap-junction inhibitors (CBX, FFA), purinergic P2 antagonists (SUR, MRS2179), agonist 2-Me-S-ADP and Ca2+ store depleting conditions (zero-added Ca2+ with or without CPA) were investigated. To start with, the number of cells participating in the first (100 μM ATP) and the second (100 μM ATP plus test compound) ATP applications were compared (Figure
Figure 4
Molecular dissection of ATP-evoked burst-like Ca2+ signalling in the NAc slice
Molecular dissection of ATP-evoked burst-like Ca2+ signalling in the NAc slice. A: Cell number-based profiling. Left - ATP-evoked of Ca2+ transients: Effects of gap-junction blockers CBX, FFA; P2 antagonists SUR and MRS2179; Na+ channel blocker TTX; Ca2+ store depletor CPA with/without added external Ca2+. Right - 2-Me-S-ADP evoked Ca2+ transients: Effect of MRS2179. B: Fluorescence intensity-based profiling. Left - ATP-evoked of Ca2+ transients: Effects of gap-junction blockers CBX, FFA; P2 antagonists SUR and MRS2179; Na+ channel blocker TTX; Ca2+ store depletor CPA with/without added external Ca2+. Right - 2-Me-S-ADP evoked Ca2+ transients: Effect of MRS2179. C: Correlation of data obtained through cell number- and fluorescence intensity-based evaluation of Ca2+ transients. The effects of tested compounds/conditions were compared to the control using Mann-Whitney with Bonferroni
In addition to the number of ATP-responsive cells, we also analysed the magnitude of the response (Figure
SUC activates Ca2+ transients in NAc astrocytes
We tested the hypothesis whether, in addition to ATP, SUC also activates astroglial Ca2+ signals. This was explored in measurements of the effect of SUC alone and in combination with the ATP puff-evoked Ca2+ transients characterized before. To this end, we applied the drug testing protocol described before (
Indeed, superfusion of SUC initiated Ca2+ transients in a sub-population of NAc astrocytes showing Ca2+ transients in response to puffs of ATP (Figure
Figure 5
SUC and ATP evoke overlaying Ca2+ transients in NAc astrocytes
SUC and ATP evoke overlaying Ca2+ transients in NAc astrocytes. A1: Confocal image of SUC-responsive cells (green and red circled yellow spots) participated in ATP-stimulated concerted Ca2+ burst (green spots marked by white arrows). Red arrows show directions of SUC (vertical) and ATP (horizontal with x) applications. Scale bar is 50 μm. A2: Representative (dF/F0)max plots showing effects of 50 μM SUC alone and with 100 μM ATP puff in A1. Red and green traces correspond to the SUC-responsive cells circled in A1 while gray traces show the fluorescence changes of cells marked by white arrows in A2. B: Confocal images showing fluorescence of 5 μM Fluo-4 AM loaded cells in NAc slices during control (left image), 50 μM SUC application (middle images) and washout (right image). Time refers to the fluorescence-time plot (C). Scale bar is 50 μm. C: (dF/F0)max plots showing Ca2+ transients in response to 50 μM SUC in the NAc slice. Each coloured trace corresponds to a Ca2+ transient circled in (B). D: Comparison of cell number- and fluorescence intensity-based summary plots of the effects of SUC on Ca2+ fluorescence in rat NAc slices. *
The change in the number of cells showing SUC-evoked Ca2+ transients could be characterised by an EC50 value of 50-60 μM (Figure
Next, we asked if Ca2+ transients evoked by SUC application were occurred in astrocytes. It has already been demonstrated that the protocol used here for Fluo-4 AM loading preferentially labels astrocytes
Neurons within the domain of burst-like Ca2+ signalling are not activated
In order to see if
Figure 6
Neuronal activity is not modified by ATP-responsive astroglial Ca2+ signalling in the NAc slice
Neuronal activity is not modified by ATP-responsive astroglial Ca2+ signalling in the NAc slice. A: Fluo-4/tetrapotassium (200 μM) (upper image, left; red arrow) or OGB-1/hexapotassium (50 μM) (lower image left; red arrow) salt filled neurons (B left or right images) were distinguished by the presence of the fast Na+ current in the NAc slice. They did not colocalize (A upper and lower images, right; gray arrows indicate the orientation of the long ATP puff) with the long ATP puff-induced Ca2+ transients in NAc astrocytes (white arrows). Representative gray and green (dF/F0)max traces in A show astroglial and neuronal (B) Ca2+ fluorescence, respectively. Scale bar is 50 μm. B: Higher magnification of Fluo-4/tetrapotassium (200 μM) (B left) or OGB-1/hexapotassium (50 μM) (right) filled neurons. Scale bar is 50 μm. C: Summary of the average values of characteristic properties of postsynaptic currents recorded from neurons in the NAc slice. Currents were recorded at -70 mV holding potential using K-Gluconate-based pipette solution. Peak amplitudes and frequencies of individual currents were averaged throughout 1-min intervals. Mann-Whitney test used for statistical evaluation of amplitude and frequency variations, neither of them was found to be significant (18 neurons, 10 slices, 10 rats;
Although the identified reporter neurons were flooded by the long ATP puff (
Similarly to ATP application (Figure
We described that ATP evokes burst-like propagation of Ca2+ signals amongst astrocytes in the NAc. We disclosed for the first time that SUC does also induce Ca2+ transients. We will discuss the mechanisms of Ca2+ signalling amongst astrocytes in the NAc and their possible relation to neuronal activity. The potential importance of metabolites related to brain energy state regulating Ca2+ signals will also be speculated.
Intercellular Ca2+ signalling through Cx43 containing astrocytes in the NAc
The ATP-evoked burst-like Ca2+ signal apparently propagates through astrocytes coupled by gap-junctions
Astroglial burst-like Ca2+ signals are known to propagate
In contrast, the blockade of both ATP- and the P2Y1-selective 2-Me-S-ADP-evoked Ca2+ transients by the P2Y1 selective antagonist MRS2179 indicated that the astroglial burst-like Ca2+ signal may possibly be evoked by activation of the P2Y1 receptor subtype in the NAc. The observed variability in MRS2179 effects may be related to developmental changes of P2Y1 receptors
As discussed before, Ca2+ store mobilization, underlying propagating astroglial burst-like Ca2+ transients can occur via P2Y1 receptor activation in the NAc. Both the inhibitor profile (blockade by CBX, FFA and Cx43 antibody) and dynamics of ATP-evoked Ca2+ waves suggest cell-to-cell signalling through the gap-junction-coupled astrocytes in the NAc. Low FFA efficacy may be due to the possible enhancement of intracellular Ca2+ by FFA acting at type 6 transient receptor potential canonical channel (TRPC6)
As outlined above, the alternative ATP-driven way of signal propagation
NAc astrocytes respond to SUC
SUC binding sites have previously been disclosed in rat forebrain and human NAc membrane homogenates
The concentration of SUC in plasma
Signalling layer independent of neurones
It has been reported, that mechanically evoked astrocytic Ca2+ waves mediated by the release of ATP and the activation of P2 receptors in hippocampal cultures down-regulate excitatory glutamatergic synaptic transmission in an ATP-dependent manner
Propagation of the Ca2+ signal in ellipsoidal and/or radial waveform at a speed of approximately 10 μm/s in NAc astrocytes is comparable to the time evolution of Bergmann glia Ca2+ transients, as previously recorded from fixed location in vitro
Findings obtained with the NAc model of glia activation described in this work do not exclude, however, that Ca2+ signalling involving ATP/SUC sensing cells might be a unique feature of the NAc. We may also assume such a signalling route in other brain areas exhibiting extensive Cx43 expression. The way of Ca2+ signalling is probably characteristic of a given brain region
The issue of independence as opposed to interdependence of glial and neuronal activities is related to the question of astrocytic release of signalling molecules that may also modulate synaptic transmission, a process called gliotransmission. As outlined before, there are several indications, that astrocytes do affect synaptic activity through the release of ATP, Glu, D-Ser, tumour necrosis factor alpha [
Diverse cellular pathways of astroglial Ca2+ signals are differently pronounced in various brain regions and they can also co-occur. Besides, the initial stimuli are likely a determinant of the relevant physiological function being involved.
The findings of the present study describe for the first time the Ca2+ signals evoked in astrocytes of the brain reward area, the nucleus accumbens, by the citric acid cycle intermediate SUC. We also identify these SUC-responsive Ca2+ signals in ATP-evoked burst-like intercellular Ca2+ signalling. We provide evidence that ATP- and SUC-responsive glial Ca2+ signals are independent on neuronal activity therefore apparently represent a signalling layer independent of NAc neurons.
ATP-induced calcium responses have previously been shown in cortical and hippocampal astrocytes whereas succinate-induced calcium signals had not been described in astrocytes. This previously unrecognized glial action of the major cellular energy metabolite SUC may represent a link between brain energy states and Ca2+ signalling in astrocytic networks.
Ethics statement
Animal care and preparations were in accordance with the Helsinki declaration, the European Council Directive of 24 November 1986 (86/609/EEC), the Hungarian Animal Act 1998, and were associated local guidelines, as approved by the Institutional Animal Care and Use Committee (approval ID: MÁB 1.51.4.).
Buffers and test compounds
Slice preparing buffer contained in mM: 250 sucrose, 2 KCl, 1.25 KH2PO4, 10 MgSO4, 2 CaCl2, 16 NaH2CO3 and 10 glucose. Artificial cerebrospinal fluid (ACSF) contained in mM: 129 NaCl, 2 KCl, 1.25 KH2PO4, 1 MgSO4, 2 CaCl2, 16 NaHCO3 and 10 glucose.
The following drugs were applied
Fluorescence indicators Fluo-4 AM, Fluo-4 tetrapotassium salt, OGB-1 hexapotassium salt, propidium iodide (PI), SR101 and pluronic acid were purchased from Molecular Probes (Eugene, USA). Stock solutions of ester fluorescence indicators prepared in DMSO were diluted to 0.1% DMSO in the staining solution.
Acute slice preparation and dye loading
Coronal slices from the forebrain through the NAc and the caudate putamen (CP) were prepared for the imaging experiments. Young, 10-14 day-old male Wistar rats were decapitated and the brains were quickly removed. The forebrains were serially cut into 300 μm thick coronal sections (Vibratome, Technical Products International Inc., St. Louis, MO, USA). The slices were collected in ice-cold preparation buffer and incubated for one hour under humidified gas-mixture carbogen (5% CO2 + 95% O2) atmosphere in an interface-type holding chamber containing warmed (35°C) ACSF. After preincubation in 2% pluronic acid containing ACSF for 2 minutes, slices were further incubated with 5 μM Fluo-4 AM in ACSF at 35°C in the dark under humidified carbogen (5% CO2 + 95% O2) atmosphere, for one hour
Confocal imaging and drug testing protocol
We used Fluo-4 AM for quantifying astroglial Ca2+ concentrations in the 100 nM to 1 microM range
Freshly isolated slices were transferred to the submerge-type recording chamber mounted on the stage of the microscope and were superfused with carbogenated (5% CO2 + 95% O2) ACSF (3 ml/min, room temperature). Serial scanning of slices were made at 488 nm excitation wavelengths and emitted green fluorescence was collected through a 510-530 nm bandpass filter. Ca2+ transients were initiated by application of SUC in the perfusion or by pressure-ejection of 100 μM ATP in ACSF through a glass micropipette (5-10 μm diameter) on the slice surface. Fluorescence intensity changes within a 355 × 355 μm field containing approximately 100 Fluo-4 AM loaded cells around the area of the ATP puff, were followed over a 10-minute interval (2 s/image).
Two 10-minute-long consecutive recordings were made from each slice with a 20-minute resting time between the two recordings. ATP was applied 3 min after the beginning of each 10-minute-long recording period. All tested drugs (CBX, FFA, SUR, MRS2179, CPA, TTX) and/or zero-added Ca2+ condition were applied throughout the second 10-minute-long recording period and were present at the time of the second ATP application. Co-localisation of Ca2+ transients evoked by the 1st and the 2nd ATP puffs indicated similarity of Ca2+ dynamics (Figure
Figure 7
Ca2+ transients evoked by consecutive ATP puffs applied onto the surface of the NAc slice
Ca2+ transients evoked by consecutive ATP puffs applied onto the surface of the NAc slice. A: Time series of Ca2+ transients in response of the 1st (left, red) and the 2nd (middle, green) ATP-puff applied by pressure-ejection of 100 μM ATP through a 5-10 μM diameter patch-pipette for 60 s (long puff). Time refers to the time elapsed from the beginning of the ATP applications. Overlaying Ca2+ transients (right, yellow) suggest similarity of Ca2+ dynamics of consecutive ATP puffs. Scale bar is 50 μm. B: Comparison of Ca2+ signalling after the 1st and 2nd ATP application in the corpus callosum (
Images taken during the ATP application were summed and the average of images taken during the control period was subtracted as a background. The Fluo-4 loaded cells were identified by using custom written algorithms in Matlab under strict visual control. The intensity changes in individual cells were calculated as dF/F0. To identify ATP responsive cells, the standard deviances of the control periods of the dF/F0 traces (SDcontrol) were calculated and the traces with (dF/F0)max,ATP > 5*SDcontrol were selected. The number of cells with fluorescence intensity changes above the threshold was counted to determine the number of responsive cells (N) and the average of their maximal dF/F0 values ((dF/F0)max) during the ATP application period was used to assess the magnitude of the response. The changes in N and (dF/F0)max values (ΔN and Δ(dF/F0)max, respectively) obtained in the presence of test compounds were normalized to the corresponding N and (dF/F0)max values obtained in the absence of the test compounds (Figure
Data presented are mean ± S.E.M., with N denoting the number of slices in a given experimental condition. Statistical analysis was performed using the non-parametric Mann-Whitney test with Bonferroni
Whole cell patch clamp recordings were performed both in the shell and the core areas of the NAc by using a MultiClamp 700A amplifier (Axon CNS, Molecular Devices, Sunnyvale, California, USA). Signals were low-pass filtered at 2 kHz and digitized at 10 kHz (Digidata1320A, Axon Instruments). Cells selected by their visual appearance were identified as neurons by the presence of voltage activated fast Na+ currents during application of a voltage ramp protocol -40 to +50 mV (pClamp8, Axon Instruments). 6-8 Mc pipettes pulled from borosilicate glass capillaries were filled with an intracellular solution (containing in mM: 135 K-Gluconate, 10 NaCl, 0.05 CaCl2, 2 adenosine-triphosphate Mg2+ salt, 10 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES); pH 7.3 (set with 1N KOH). In order to visualize and monitor cytosolic Ca2+ of patched neurons, 200 μM Fluo-4 tetrapotassium salt or 50 μM OGB-1 hexapotassium salt (excitation: 488 nm, emission: 510-530 nm for both dyes) was added to the intracellular solution. Cells (input resistance: 166 ± 13 MΩ) were clamped to -70 mV without corrections for liquid junction potential (-15 mV). At this potential inward spontaneous postsynaptic currents were recorded. If signs of seal deterioration or cell closure occurred, the recordings were discarded. The first 10 min of each recording were used for monitoring stabilization of the baseline. Then, a 1-minute duration control period, followed by ATP application (1 min), and a washout period (5-10 min) were recorded.
Spontaneous postsynaptic currents (PSCs) were analyzed using Mini Analysis software (Synaptosoft, Decatur, USA;
Tissue processing for immunohistochemistry
Adult, male Wistar rats (n = 3) (200-250 g body weight; Charles Rivers Laboratories, Hungary) were deeply anesthetized with a mixture containing 0.2 ml/300 g b.w. ketamine (100 mg/ml) and 0.2 ml/300 g b.w. xylazine (20 mg/ml), and perfused transcardially with 150 ml saline followed by 300 ml of ice-cold 4% paraformaldehyde in phosphate buffer, pH 7.0 (PB). Brains were removed and postfixed in the same fixative solution for 24 h, and transferred to PB containing 20% sucrose for 2 days. Serial coronal sections were cut at 50 μm on a sliding microtome (SM 2000R, Leica Microsystems, Nussloch, Germany) between +4.0 mm to 0 mm from the level of the bregma. Sections were collected in PB containing 0.1% sodium azide and stored at 4°C until further processing.
Cx43 immunohistochemistry
Free-floating sections were immunolabeled for Cx43 using an affinity-purified rabbit polyclonal antiserum raised against the carboxy terminal 362-382 peptide segment KPSSRASSRASSRPRPDDLEI of Cx43 (Abcam, Cambridge, UK, catalogue number: ab11370). Brain sections were pretreated in PB containing 0.5% Triton X-100 and 3% bovine serum albumin for 1 h. Then, they were incubated with a primary antibody against Cx43 (1:1250) in PB containing 0.5% Triton X-100 and 3% bovine serum albumin and 0.1% sodium azide for 48 h at room temperature. Sections were then incubated in biotin-conjugated donkey anti-rabbit secondary antibody at 1:1000 (Jackson Immunoresearch, West Grove, PA) for 2 h, followed by incubation in avidin-biotin-horseradish peroxidase complex (ABC) at 1:500 (Vectastain ABC Elite kit, Vector Laboratories, Burlingame, CA) for 2 h. Then, sections were treated with either 0.02% diaminobenzidine (Sigma) or fluorescein isothiocyanate (FITC)-tyramide (1:8000) with 0.003% H2O2 in Tris-HCl buffer (0.05 M, pH 8.2) for 6 min. After washing, the sections were mounted on positively charged slides (Superfrost Plus, Fisher Scientific) and coverslipped with either Cytoseal 60 (Stephens Scientific, Riverdale, NJ, USA) or in antifade medium (Prolong Antifade Kit, Molecular Probes).
Cx43 and GFAP double immunohistochemistry
Free-floating sections were first immunolabeled for Cx43 using FITC-tyramide amplification immunohistochemistry, as described above. Then, sections were incubated overnight in a mouse monoclonal anti-GFAP, a marker of astrocytes (1:250; catalogue number: sc-33673, Santa Cruz Biotechnology, Santa Cruz, CA). Subsequently, sections were incubated in Alexa 594 donkey anti-mouse secondary antibody (1:500; Molecular Probes) for 2 h, then mounted and coverslipped, as described above. Sections were examined using an Olympus BX60 light microscope also equipped with fluorescent epi-illumination. Images were captured at 2048 × 2048 pixel resolution with a SPOT Xplorer digital CCD camera (Diagnostic Instruments, Sterling Heights, MI) using 4-20× objectives. Confocal images were acquired with a Nikon Eclipse E800 confocal microscope equipped with a BioRad Radiance 2100 Laser Scanning System using 20-60× objectives (at an optical thickness of 1-3 μm). Contrast and sharpness of the images were adjusted using the "levels" and "sharpness" commands in Adobe Photoshop CS 8.0. Colours were adjusted so that Cx43 appeared red and GFAP was green. Full resolution was maintained until the photomicrographs were cropped and assembled for printing, at which point images were adjusted to a resolution of 300 dpi.
Post-calcium imaging immunohistochemistry protocol for Cx43 and GFAP
In order to identify the cell types involved in Ca2+ bursts, the brain slices were immunostained with antibodies for astrocyte marker proteins (Cx43 and GFAP). However, Fluo-4 signal could not be preserved through fixation with either 0.4% paraformaldehyde or 40 mg/ml EDAC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, Sigma-Aldrich; Dawling and Deitmer, 2002). Therefore, co-localization between SUC/ATP-responsive cells and cell type markers could not be resolved. Hence we opted to perform the
Authors' contributions
TM carried out the calcium imaging studies and drafted the manuscript. TM and LH performed the statistical analysis. AD carried out the immunoassays. GNY performed the electrophysiological studies. PB participated in the design of the study and data analysis. LH, EZS and MP participated in the interpretation of data and helped to draft the manuscript. JK conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
The authors thank Erzsébet Kútiné Fekete and Ildikó Pál (Department of Neurochemistry, Institute of Biomolecular Chemistry, Chemical Research Center, Hungarian Academy of Sciences) for excellent technical assistance and interpretation of data, respectively. Authors TM, GNY, PB, LH, ZSE and JK were supported by grants 1/A/005/2004 MediChem2, GVOP-3.2.1.-2004-04-0210/3.0 Transporter Explorer AKF-050068, TECH-09-AI-2009-0117 NanoSen9 and CRC-HAS-2009-Nanotransport. Authors AD and MP were supported by the grant TECH-09-AI-2009-0117 NanoSen9.
Affective neuroscience of pleasure: reward in humans and animals
Regional and subcellular localization in rat brain of the enzymes that can synthesize gamma-hydroxybutyric acid
γ-Hydroxybutyrate (GHB) induces GABAB receptor independent intracellular Ca2+ transients in astrocytes, but has no effect on GHB or GABAB receptors of medium spiny neurons in the nucleus accumbens
Gamma-hydroxybutyrate binds to the synaptic site recognizing succinate monocarboxylate: a new hypothesis on astrocyte-neuron interaction via the protonation of succinate
Metabolic GHB precursor succinate binds to gamma-hydroxybutyrate receptors: characterization of human basal ganglia areas nucleus accumbens and globus pallidus
Validation of high-affinity binding sites for succinic acid through distinguishable binding of gamma-hydroxybutyric acid receptor-specific NCS 382 antipodes
Motor behavior activates Bergmann glial networks
Intercellular calcium signalling in astrocytes via ATP release through connexin hemichannels
Brief bursts of parallel fiber activity trigger calcium signals in Bergmann glia
Glutamatergic and purinergic receptor-mediated calcium transients in Bergmann glial cells
Calcium dynamics of cortical astrocytic networks in vivo
Cortical layer 1 and layer 2/3 astrocytes exhibit distinct calcium dynamics in vivo
Radially expanding transglial calcium waves in the intact cerebellum
Extracellular ATP triggers two functionally distinct calcium signalling pathways in P C12 cells
Müller cell Ca2+ waves evoked by purinergic receptor agonists in slices of rat retina
The role of metabotropic glutamate receptors for the generation of calcium oscillations in rat hippocampal astrocytes in situ
mGluR5 stimulates gliotransmission in the nucleus accumbens
Increase of intracellular Ca2+ by P2X and P2Y receptor-subtypes in cultured cortical astroglia of the rat
Segregated expression of AMPA-type glutamate receptors and glutamate transporters defines distinct astrocyte populations in the mouse hippocampus
Developmental profile and properties of sulforhodamine 101-labeled glial cells in acute brain slices of rat hippocampus
The C-terminus of connexin43 adopts different conformations in the Golgi and gap-junction as detected with structure-specific antibodies
Functional hemichannels in astrocytes: a novel mechanism of glutamate release
New evidence for purinergic signalling in the olfactory bulb: A2A and P2Y1 receptors mediate intracellular calcium release in astrocytes
Spontaneous astrocytic Ca2+ oscillations in situ drive NMDAR-mediated neuronal excitation
Nuclear calcium signalling evoked by cholinergic stimulation in hippocampal CA1 pyramidal neurons
New roles for astrocytes: gap-junction hemichannels have something to communicate
Glial calcium signalling in physiology and pathophysiology
Activity-dependent ATP-waves in the mouse neocortex are independent from astrocytic calcium waves
Gap-junctions in the adult cerebral cortex: regional differences in their distribution and cellular expression of connexins
Immunocytochemical characterization of a new marker of fibrous and reactive astrocytes
Connexin expression by radial glia-like cells is required for neurogenesis in the adult dentate gyrus
Mechanical stimulation and intercellular communication increases intracellular Ca2+ in epithelial cells
An extracellular signalling component in propagation of astrocytic calcium waves
Intercellular calcium signalling mediated by point-source burst release of ATP
Pharmacological characterization of heterologously expressed ATP-gated cation channels (P2X purinoceptors)
Receptors for purines and pyrimidines
Molecular pharmacology of P2Y-receptors
Extracellular ATP stimulates adenylyl cyclase and phospholipase C through distinct purinoceptors in NG108-15 cells
Activation of the skeletal muscle ryanodine receptor by suramin and suramin analogs
Modification of the conductance and gating properties of ryanodine receptors by suramin
Physiology and pathophysiology of the calcium store in the endoplasmic reticulum of neurons
Novel consequences of voltage-dependence to G-protein-coupled P2Y1 receptors
Regulation of intracellular Ca2+ by P2Y1 receptors may depend on the developmental stage of cultured rat striatal neurons
Succinate reverses in-vitro platelet inhibition by acetylsalicylic acid and P2Y receptor antagonists
Functional distinctions in cytosolic calcium regulation between cells of the glomerular filtration barrier
Angiotensin II-stimulated Ca2+ entry mechanisms in afferent arterioles: role of transient receptor potential canonical channels and reverse Na+/Ca2+ exchange
Gamma-hydroxybutyrate, a normal brain metabolite
GABA, gamma-hydroxybutyric acid, and neurological diseases
From the street to the brain: neurobiology of the recreational drug gamma-hydroxybutyric acid
Use of GHB compounds among college students
Calcium waves in astrocytes-filling in the gaps
An expressed sequence tag (EST) data mining strategy succeeding in the discovery of new G-protein coupled receptors
Citric acid cycle intermediates as ligands for orphan G-protein-coupled receptors
Anatomical profiling of G protein-coupled receptor expression
Localization of the succinate receptor in the distal nephron and its signaling in polarized MDCK cells
Succinate modulates Ca2+ transient and cardiomyocyte viability through PKA-dependent pathway
Chemical composition of blood plasma and serum
Organic acids in blood and urine
Analysis of dicarboxylic acids by tandem mass spectrometry. High-throughput quantitative measurement of methylmalonic acid in serum, plasma, and urine
Succinate accumulation in man during exercise
Circulating anions usually associated with the Krebs cycle in patients with metabolic acidosis
Circulating succinate is elevated in rodent models of hypertension and metabolic disease
Synchronization of Ca2+ oscillations: involvement of ATP release in astrocytes
Dynamic inhibition of excitatory synaptic transmission by astrocyte-derived ATP in hippocampal cultures
Astrocytic purinergic signaling coordinates synaptic networks
Astrocytic activation of A1 receptors regulates the surface expression of NMDA receptors through a Src kinase dependent pathway
Astrocyte Ca2+ waves trigger responses in microglial cells in brain slices
Mechanisms of spreading depression and hypoxic spreading depression-like depolarization
Immature neocortical neurons exist as extensive syncitial networks linked by dendrodendritic electrical connections
Calcium waves in retinal glial cells
Intercellular communication in spinal cord astrocytes: fine tuning between gap-junctions and P2 nucleotide receptors in calcium wave propagation
TNFα controls glutamatergic gliotransmission in the hippocampal dentate gyrus
Glutamate uptake triggers transporter-mediated GABA release from astrocytes
Contribution of astrocytic glutamate and GABA uptake to corticostriatal information processing
Hippocampal short- and long-term plasticity are not modulated by astrocyte Ca2+ signalling
Selective stimulation of astrocyte calcium in situ does not affect neuronal excitatory synaptic activity
Long-term potentiation depends on release of D-serine from astrocytes
Hippocampal astrocytes in situ respond to glutamate released from synaptic terminals
Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes
ImageJ 1.44 image analysis software


text: You guys are amazing. How we handled the new chapter. It's all edited so fast and correctly, I feel like we were really working as a team today. Great job, I'd like give you all an e-pad on the shoulder. (Not to be confused for an iPad, those are a bit expensive you know).


text:
- Overview
- Biography
- Songs
- Browse by Format
- Browse by Genres
- Similar Artists
|Pop / Rock
|progressive rock classic rock rock 70s british
|1970s 1980s 1990s 2000s
|allmusic discogs lyrics lyrics other databases wikidata wikipedia
When the 60s pop group the Zombies finally disintegrated in 1969, keyboard player Rod Argent (Rodney Terence Argent, 14 June 1945, St. Albans, Hertfordshire, England) wasted no time in forming a band that would enable his dexterity as a pianist and songwriter to flourish. The assembled unit also included Russ Ballard (b. 31 October 1945, Waltham Cross, Hertfordshire, England; guitar/vocals), Bob Henrit (b. Robert Henrit, 2 May 1944, Broxbourne, Hertfordshire, England; drums) and Jim Rodford (b. 7 July 1941, St. Albans, Hertfordshire, England; bass). Their critically acclaimed 1970 debut included Ballards Liar, a song that became one of the bands concert regulars and was also a US Top 10 hit for Three Dog Night in 1971, the year Argents second album Ring Of Hands was released. 1972s All Together Now featured the exhilarating Hold Your Head Up (co-written by Argent and former Zombies bass player Chris White), which...
- MatchArgent
- 100%Atomic Rooster
- 100%Emerson, Lake & Palmer
- 98%Curved Air
- 97%Family
- 89%Wishbone Ash
- 83%Barclay James Harvest
- 81%Caravan
- 76%Bachman
- 76%Bachman-Turner Overdrive
- 75%Renaissance


text:
Otto Marine Limited says that its shipyard has, through one of the Company's wholly-owned subsidiaries sold an Anchor Handling Tug Supply Vessel, (the "Vessel"), to a renowned unrelated third party (the "Buyer") for US$100 million.
The Vessel is currently under construction at the Company's shipyard in Batam, Indonesia and will be handed over to the Buyer following the completion of legal documentation and a final inspection.
Upon the completion of the Vessel, the Vessel would be chartered by the Company's subsidiary, GO Offshore (L) Private Limited, for a period of 8 years.
Save as disclosed above, none of the directors or controlling shareholders of the Company has any interest, whether direct or indirect, in the above transactions other than through their shareholdings in the Company.
The transaction above is expected to have a positive impact on the consolidated net tangible assets per share and earnings per share of the Group for the financial year ending 31 December 2014.
Otto Marine Limited
Please be civil. Job and promotion will not be added into the comment page.
Leave this field blank
Your name*
Your e-mail*
Your website
Leave this field blank
Your comment*
(Use Markdown for formatting.)
This question helps prevent spam:
What color is the sky on a clear day?*
Remember me
your browser.
Schlumberger has announced an agreement to acquire a majority (51%) equity interest in Eurasia Drilling Company Limited.
Mobile news
Our news
your website
Do you have any
tips to us
About us | Contact | Privacy | Terms |
Editor: Michael Rogers
Copyright 1973-2016. Scandinavian Oil-Gas Online. ISSN 1500-709X.
Tel: +47 22 44 72 70, Fax: +47 22 44 72 87


text:
12月 13, 2014 合計視聴数:: 5224 今日は: 1 ★★★★
Hotel Mantra Sierra Grand Gold Coast, Mantra Sierra Grand is located within 3 minutes' stroll from the Braodbeach and surf beaches. It offers luxurious, self-contained accommodation with panoramic ocean and hinterland views from some rooms. ザ 1, 2 and 3-bedroom apartments feature full kitchen and laundry facilities, open-plan living rooms and wide balconies. Wireless internet is also available. Guests can take advantage of the indoor and outdoor swimming pools and spa, work out in the fitness centre or watch a movie at the on-site theatre. Mantra Sierra Grand is only a 10-minute walk from Jupiters Casino, The Gold Coast Convention Centre and Pacific Fair shopping centre. (Hotel Mantra Sierra Grand ゴールドコーストのホテル 予約).


text:
Lada 4X4
The Lada Niva Russian off-road vehicle has been marketed in most export countries as the Lada 4x4 since 2009. At the time of inception, the 4x4 was the first mass-production off-road vehicle to feature unibody architecture, independent front suspension and coil suspension.
The problem of stone chips and road rash
Stone chip damage is caused when grit and loose stone chips are sprayed up off the road and hit the bodywork of cars, leaving nasty scratches in the paint. Another name for this damage is road rash.
What is Chipex Lada 4x4 Touch up Paint?
Chipex Lada 4x4 Touch up Paint is an advanced and superlative antidote to stone chip paint scratches.
Why is Chipex better than traditional methods?
Chipex guarantees perfectly matching paint or your money back.
Ready-to-apply paint, clear, easy instructions, an option of three sizes and all-inclusive accessories come as standard.
The seamless effects of a Chipex repair will leave you astounded.
Chipex is much more affordable than a repair by a professional paint scratch repair company.
The problem of stone chips and road rash
The roads habitually spit grit and loose stone chips at the bodywork of cars, causing a spray of paint scratches to form. This is called road rash.
What is Chipex 4X4 Touch up Paint?
Chipex 4X4 Touch up Paint is the road rash repair solution you've been waiting for.
Why is Chipex better than traditional methods?
- Chipex offers a unique colour match guarantee - it promises paint that's exactly matched with the colour code of your 4X4.
- Standard inclusions are easy-to-follow instructions, all the accessories you need and ready-to-apply paint.
- The flawless finish makes a full respray an expensive and unnecessary task.
- Unlike repairs by professional paint scratch repair companies, you don?t need deep pockets to get a Chipex repair.


text:
Video: Jeremy Powers' Book of Cross, chapter 1
Jeremy Powers talks about CrossVegas, his struggles with ADD, and the 2016 cyclocross season.
Tour de France reports, opinion, and photos Read More »
Jeremy Powers talks about CrossVegas, his struggles with ADD, and the 2016 cyclocross season.
Ryan Trebon rides from the back of the CrossVegas World Cup field to earn a top-15 finish after overcoming a 2014 back injury.
With two riders in the lead group, the Luna team sets up Katerina Nash to win the opening World Cup of the cyclocross season.
Wout Van Aert attacks early and rides a brilliant solo move all the way to victory in the first cyclocross World Cup of the season.
Results from the season-opening race on the cyclocross World Cup, won by Wout Van Aert and Katerina Nash.
Wout Van Aert opens his World Cup cyclocross account in Las Vegas, earning a convincing solo victory Wednesday night.
Katerina Nash takes a flyer on the second-to-last lap, and her bold move pays off with a big win at the first CX World Cup in the U.S.
Due to a knee injury suffered at the Tour de l'Avenir, reigning 'cross world champion Mathieu van der Poel will miss CrossVegas.
USA Cycling announces the elite men and women invited to race the first-ever North American cyclocross World Cup in Las Vegas.
Improbable as it seemed, CrossVegas, a race run in the middle of Nevada's most infamous desert metropolis, will become a World Cup race


text:
SMALLRIG Wooden Handle (Left Side) for DSLR Cage 1738 is extremely handcrafted rosewood grip and specially attached to camera cages by two 1/4''threaded screws. It is compatible for SmallRig universal DSLR cages, such as quick cage 1510, VersaFrame Cage 1584. Bolt it to the left side bar of the cage, you get a comfortable wooden grip for smooth operation. It is well-built and elegant.
Compatible with:
quick cage 1510, quick cage 1535, VersaFrame Cage 1584, VersaFrame Cage 1630, Half-VersaFrame Cage 1658
1x wooden handle
2x 1/4'' screws
1x hex spanner
Net Weight: 78g


text:
DPS Recruitment 2015
Delhi Public School has published a job notification as DPS Recruitment 2015 to hire the capable contenders for teaching and non- teaching posts. Interested and eligible participants, who are willing to make their career in this well known school, can apply for DPS Job Notification by submitting their application form before the closing date which is given below.
Important Date:
|Application form available
|8th to 15th April 2015
To get more essential information about DPS Recruitment 2015 such as post name, qualification criteria, selection process, how to apply etc you need to go through the info are well mentioned on this page by the team of bharatrecruitment.com
Name of the Post:
- Teaching staff (regular): PGT: Mathematics, Biology, Economics, Physics, Chemistry
- TGT: Physical education, Counselor
- PRT: General, Physical education
- Lab attendant: For biology lab, General lab
- Non- Teaching staff: Driver, Estate officer
- Temporary vacancies: Commerce teacher, Physical Education teacher, Theatre Expert (dramatics), Western Music Teacher ( Vocal and Instrumental), System Administrator (I.T), Asst. Hostel Warden, Swimming Coach/ Life Guard (Preferably Lady), Tabla Player, Cook Electrician, Electrical Engineer, Physical Fitness Instructor.
Qualification Criteria:
The qualification of the selected applicants must have passed Graduate/Post Graduate degree/ B.Ed degree in relevant discipline from the recognized university or institute.
Mode of Selection:
Selection of those contenders who are applying for DPS jobs will be made on the basis of the performance of their interview which will be conducted by the Delhi Public School.
Interview Tips For Freshers / Exp.
How to Apply For Delhi Public School Recruitment 2015?
- Interested aspirants who want to apply need to visit official website that is www.dpsmathuraroad.net
- Then, on the homepage, select the "vacancy" tab.
- Now, read full information carefully and fill all the necessary details with correct manner.
- The, press on "submit detail and download form" button.
- After that, attach photocopies of all essential document/testimonial and passport size photographs.
- At last, send their application form to the below address within a given time period.
Postal Address:
Delhi Public School,
Mathura Road,
New Delhi – 110003
Important Date:
Application form will be available from – 8th to 15th April 2015
Note: Aspirant can check Official Website, for more detail against DPS Recruitment 2015.
You may also like to Read


text:
Give us a call to get our secret deals 1-800-468-3578
Property Location
A stay at Meliá Campione places you in the heart of Campione d'Italia, steps from Casino Municipale di Campione d'Italia and close to Monte San Salvatore. This 5-star hotel is within close proximity of Swissminiatur and Museum of Modern Art.
Make yourself at home in one of the 40 air-conditioned rooms featuring iPod docking stations and flat-screen televisions. Complimentary wired and wireless Internet access keeps you connected, and satellite programming provides entertainment. Private bathrooms have rainfall showerheads and designer toiletries. Conveniences include phones, as well as safes and desks.
Treat yourself with massages, body treatments, and facials. Additional amenities at this hotel include complimentary wireless Internet access and concierge services.
Satisfy your appetite at the hotel's restaurant, which serves breakfast, lunch, and dinner, or stay in and take advantage of 24-hour room service. Quench your thirst with your favorite drink at a bar/lounge.
Business, Other Amenities
Featured amenities include complimentary newspapers in the lobby, dry cleaning/laundry services, and a 24-hour front desk. Self parking (subject to charges) is available onsite.


text: McCall's 3028; ©1987; French Connection - Misses' Unlined Jacket Top Skirt: Front buttoned jacket with collar has self-lined back yoke with forward shoulder seams, extended shoulders, long two-piece sleeves with buttoned cuffs, pleated patch pockets with buttoned flaps, back insets and elastic in waistline casing. Pullover cropped top has extended shoulders. Straight skirt has front inset pockets, elastic in waistband casing, belt carrier, fly front zippered opening, back yoke with decorative carrier, optional back welt pocket with buttoned flap and back vent. All garments have top-stitching and machine-stitched hems.


text:
South of Duluth and southwest of Marksville in Pine County, the St Croix State Forest has 25 miles of trails shared by hikers and horseback riders. The trails can be accessed by either campground. The trail runs from Boulder Campgroun along the west bank of the Tamarack River, past the equestrian campground, crosses the river and then parallels the river in a southerly direction to Pine Point near the confluence of the Tamarack and the St. Croix Rivers.
Note, the horse trails along the Tamarack River bluff can be very dangerous for novice riders. Mallard Lake trails are easier.
Overall, St Croix State Forest is the 5th most popular horse trail of all 12 horseback rides in Minnesota.


text:
Mongaup Valley Wildlife Management Area
- Open: Year-round
- Fee: None
- Contact:
- DEC Region 3 Office: 845-256-3098; email@example.com
- Backcountry Emergencies: 518-408-5850 or dial 911
- State Land Law Enforcement: 845-256-3013
- Location: Towns of Forestburgh, Highland and Lumberland in Sullivan County and Town of Deerpark in Orange County
- Maps: Download a map of Mongaup Valley Wildlife Management Area (PDF) (582 KB) || Google Earth || State Lands Interactive Mapper
Our national symbol, the bald eagle, is the star of this wilderness about 75 miles northwest of New York City. The Mongaup Valley Wildlife Management Area (WMA) contains 6,313 acres of land owned by the State of New York and 5,542 acres of land on which the State owns conservation easements. Mongaup has been designated a Bird Conservation Area because of its unique combination of habitats important to bird species, including forests, reservoirs and river habitat, where the majestic birds nest and overwinter.
Featured Activities
White-tailed deer, turkey, ruffed grouse, coyote, fox, porcupine, black bear and waterfowl are found at the WMA. See hunting regulations and hunting seasons in New York.
The Mongaup River between and below the reservoirs is an excellent wild trout fishery. The reservoirs and various other bodies of water on the WMA contain as many as 42 different species of fish including smallmouth bass, largemouth bass, crappie, pan fish and stocked and wild trout. See the statewide fishing regulations (leaving DEC website to official Fishing Regulations Guide vendor website) and special fishing regulations for Sullivan (leaving DEC website to official Fishing Regulations Guide vendor website) and special fishing regulations for Orange Counties (leaving DEC website to official Fishing Regulations Guide vendor website).
Watchable Wildlife
Eagle-viewing blinds are located on Mongaup Falls Reservoir and Rio Reservoir, allowing visitors to watch the eagles without disturbing them. Winter is the best time to watch. Wintering eagles arrive in December with concentrations peaking in January and February. Eagles are most active between 7am to 9am and 4 pm to 5 pm. Scan the tree-tops for eagles perched there or watch the sky. Eagles like heavily wooded areas near water with tall trees for nesting and perching. They eat a lot of fish so check ice flows or river islands for eagles enjoying a meal. Visit the Bald Eagle Viewing page for more helpful tips.
Waterbodies and wetlands add to the diversity of Mongaup's landscape and wildlife. Rare floodplain forest, perched bog, and pitch-pine and oak-hickory woodland habitats are also found at Mongaup, attracting wetland birds and species that prefer sedge meadows.
Wildlife to Watch
- Migrating raptors--bald eagles, goshawks, turkey vultures, red-tailed, red-shouldered and sparrow hawks
- Great-horned, barn, barred and screech owls
- Woodcocks, redhead, ring-necked and canvasback ducks
- White-tailed deer, black bears, porcupines, gray and red foxes, otters, beavers, coyotes
- Eastern timber rattlesnakes, spotted salamanders
Where to Watch
- Two eagle-viewing blinds
- Forested hills, floodplain forest, perched bog
- Pitch pine-oak-heath woodland
Boat Launch (ramp)
Boat launches are available on Swinging Bridge Reservoir, Mongaup Falls Reservoir and Rio Reservoir.
Boat Launch (hand)
Canoes and rafts can be launched on the Delaware River from an access site on Route 97 just west of the intersection with County Route 31. There is also a hand launch on the southern end of the Rio Reservoir. There are special regulations for the use of Cliff Lake, where fishing is allowed from a boat but you must carry your boat approximately one mile to the launch point.
Accessible Features
General information on accessible recreation includes links to other locations with accessible recreation opportunities and information on permits for motorized access.
Both eagle-viewing blinds are accessible. There is accessible parking at both viewing blind locations and a wooded access path to the Plank Road blind.
The WMA is located in southwestern New York approximately 75 miles northwest of New York City. It's bordered by the Delaware River at its southernmost point and surrounded by State Routes 42 to the east, 17B to the north, and to the west by State Route 55 and Sullivan County Routes 32 and 31.
Eagle-viewing Blinds
There are two eagle-viewing blinds on the WMA, both with parking areas. Visitors should view eagles either from their vehicles or the blinds, rather than on foot, to avoid disturbing the eagles.
- Rio Reservoir Blind is located on Plank Road on the northeastern end of the reservoir (41.52305°N, 74.76117°W) Google Maps (leaves DEC website).
- Mongaup Falls Reservoir Blind is located on County Rt. 43 (Forestburgh Road) on the northern end of the reservoir (41.5547981°N, 74.785202°W) Google Maps (leaves DEC website).
Parking Areas and Boat Launches
There are numerous parking areas within the WMA - see map for all locations.
- Rio Reservoir (southern end) parking area and hand boat launch on Rio Dam Road (41.478637°N, 74.75545°W) Google Maps (leaves DEC website)
- Rio Reservoir trailered boat launch and parking area (41.521579°N, 74.760375°W) Google Maps (leaves DEC website)
- Mongaup Falls Reservoir trailered boat launch and parking area (41.553569°N, 74.779294°W) Google Maps (leaves DEC website)
Rules, Regulations and Outdoor Safety
The following acts are not permitted at the Mongaup Valley Wildlife Management Area:
- Parking of motor vehicles in other than designated parking areas or beyond the quotas of the parking areas.
- Operating a motor vehicle on the area except on designated access roads. Off road vehicles are prohibited on the area.
- Except on Swinging Bridge Reservoir, use of gasoline powered motors on boats, canoes and other watercraft. Electric motors are permitted. There are no restrictions on motors on Swinging Bridge Reservoir.
- Launching of vessels other than from designated launches.
- Overnight mooring or storage of boats.
- Discharging of firearms except at wildlife which may be legally hunted.
- Cutting, damaging or removal of any living vegetation or standing dead vegetation.
- Swimming or bathing.
- Setting or maintaining a fire.
- Camping.
- Littering.
- Coming within 200 feet of any dam.
- Entering onto the surface of the water or ice on the Mongaup River or Swinging Bridge, Mongaup Falls, or Rio Reservoirs or entering the upland surrounding those waters as designated by posted signs from December 1st through March 31st.
Special Regulations for Cliff Lake:
Cliff Lake, located to the north of Mongaup Falls Reservoir, and the surrounding uplands do not belong to the State of New York but are private property. Fishing easements have been granted that allow access for those fishing on the shore or from a boat. Please follow the regulations below for its use:
- Pedestrian access to Cliff Lake is permitted from May 1st through November 30th for fishing only.
- Access to and use of Cliff Lake is prohibited from ½ hour after sunset to ½ hour before sunrise.
- Vessels may be launched and retrieved only from the designated boat access site. Shoreline fishing is allowed only from the designated boat access site.
Outdoor Safety Tips
How We Manage Mongaup Valley WMA
The WMA is managed for the protection and conservation of bald eagles and their habitats. Access to many parts of the property is restricted from December 1 through March 31 each year to minimize disturbances to wintering bald eagles. Please be aware of the bright yellow or pink restricted area signs around the reservoirs and water courses and abide by them.
This WMA also contains the Mongaup Valley Bird Conservation Area.
The public access and wildlife management programs are designed to protect and enhance this unique resource for future generations. Your compliance with the area's regulations is an important step toward this goal.
Nearby State Lands to Visit
- Neversink Unique Area and Wolf Brook Multiple Use Area are a short drive to the east. The Neversink Unique Area features a large gorge and many waterfalls.
- Huckleberry Ridge State Forest is just to the south and east and features trails that link to the Shawangunk Ridge trail.
- Hickok Brook Multiple Use Area is a short distance to the west.
Tourism Information
The following links leave DEC's website.