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Meet Wheelock's Passion Scholars
Learn More about the PFAS Program
Class of 2020
Anthony Buonopane
Anthony lives in Revere, MA, and graduated from Revere High School, where he was an athlete and active volunteer. He is a recipient of the Jewish War Veterans of the United States of America memorial scholarship award. Anthony volunteers as a special education basketball coach for the Challenger League in Wakefield, MA. At Wheelock, he plans to double major in Special Education and Political Science, with a minor in Leadership. Anthony hopes to become a special education teacher, but he also has an interest in politics.
Daniela Ramirez
Daniela lives in Boston and graduated from Boston Community Leadership Academy, a pilot high school in the Boston Public Schools system. Daniela was an active volunteer at her local church, serving as a spiritual mentor for teenagers going through the confirmation process. She also was involved with an organization called buildOn that provides international service learning opportunities to high school students. She traveled to Malawi in Africa, to construct a school and she learned the value and beauty of humanitarian work and working together. At Wheelock, Daniela plans to enroll in the Child Life Dual Degree Program with a minor in Visual Arts so she will be well prepared to help children and families in need. She also hopes to be able to have more service learning travel experiences during her time in college.
Paige Spence
Paige, from South Boston, graduated from Boston Latin Academy. She was involved in a number of volunteer and extracurricular activities while in high school, including softball, swimming, Keystone Club, and work at the South Boston Boys and Girls Club. Paige is passionate about working with children, particularly being a mentor and supportive influence for those in need. At Wheelock, she plans to major in Elementary Education, with minors in Understanding Autism and Leadership. She hopes to be an elementary school teacher.
Soyini Wilcox
Soyini is from Mattapan, MA, and graduated from New Mission High School in Hyde Park. During high school, she was involved in the Gay Straight Alliance, and served on the Boston Student Advisory Council (BSAC) through the Mayor's Office. Soyini's most rewarding service experience was teaching children through Teach Boston. She has known that she wants to be a teacher since the third grade and considers working with children to be her life's passion. At Wheelock, she plans to double major in Elementary Education and Math/Science, with a minor in Understanding Autism. She has already gotten involved in Jumpstart, Campus Activities Board (CAB), and Teach, Learn, Create (TLC).
Class of 2019
Carolyn Lopez
Carolyn, who grew up in Chelsea, MA, graduated from Cristo Rey High School in Boston, where she developed and co-led the first cooking class at the school during her sophomore year. She spent time volunteering at Ellis Memorial, a nonprofit providing inner-city working families with high-quality education, and care for children, youth, and vulnerable adults. While volunteering at Ellis she began working with younger children, which has turned into the driving passion for her future career. At Wheelock, Carolyn plans to major in early childhood education in hopes of making a difference in younger children's lives as they develop. In her spare time, she enjoys playing lacrosse, singing, and dancing.
Laura Mejia
Laura is from East Boston. She graduated from Boston Latin Academy where she was heavily involved in Talented and Gifted (TAG), a program designed to ensure that Latino and English Language Learners excel academically, socially, and personally, so they can succeed in high school and at the postsecondary level. She also volunteered at Massachusetts General Hospital and taught Sunday school at her church. She plans to major in elementary education because she hopes to have an impact on the daily lives of children.
Lisvet Peralta
Lisvet, from Jamaica Plain, MA, graduated from City on a Hill Charter Public School. While she attended, she spent time volunteering at the YMCA on Huntington Avenue, playing on the volleyball team, and participating in the Art Club. She plans to major in psychology at Wheelock. She is interested in working with children who grew up dealing with bullying or discrimination.
Meghan Rubadou
Meghan, from Taunton, MA, is deeply passionate about her religion and has used this passion to impact different areas of her community through her volunteer work. As a short-term missionary, she traveled to Guatemala with her team from Christ Community Church and took part in building resources for the village of El Arco. While in Guatemala she found a love caring for and interacting with children in hospitals. Now a graduate of Taunton High School, she intends to major in child life. With a growing passion for missions and travel, she's looking forward to returning to Guatemala and other locations. In her spare time, Meghan enjoys the performing arts and creating visual art.
Class of 2018
Freddy Mata
Freddy is deeply passionate about racial equality and has started a group that helps minority students with their college applications, which he hopes to continue while a Wheelock student. Freddy is a first-generation college student from Randolph, MA. He graduated from Cristo Rey Boston High School, a college-preparatory school for low-income students. Since the age of 10, he has been involved in service at his church, and he helps teach Sunday school. He volunteers in many youth programs, as a math tutor, and as a mentor to younger high school students. During his junior year, Freddy took an international trip with his school to Nairobi, Kenya, where he uncovered his passion for service. He is planning to double major in Communications & Media Literacy and American Studies.
Sara Mendez
Sara graduated from Boston Community Leadership Academy, a pilot high school in the Boston Public Schools system. She is passionate about Wheelock's mission, improving the lives of children and families, and would like to assist those who are homeless or lacking a support system. Sara has always had an inner passion for service, but an experience during her junior year of high school solidified her calling. Working with buildOn, a nonprofit organization whose mission is to break the cycle of poverty, illiteracy, and low expectations through service and education, Sara volunteered in multiple locations monthly. Her most empowering service experience was when she traveled to Senegal to help build a school in a village, and learned about a new culture and the importance of education. When she returned to school, she became part of Inquilinos Boricuas en Acción (IBA), a program that empowers individuals through education, workforce development, and arts programs and creates vibrant affordable housing communities. Sara was motivated to come to Wheelock because of its mission and commitment to service and social justice, and she plans to study Social Work.
Carishmar "Cari" Moore
Cari graduated from the Commonwealth School in Boston. Her passion is working with children. During high school, she volunteered at Excel Charter School and Beacon Academy and served on her school's Diversity Committee. Cari was a pivotal member of the committee and represented her school at the national Student Diversity Leadership Conference. She also helped the Commonwealth School to engage in challenging conversations about race and class. Her favorite service experience was through the Amigos de las Américas program, where she was able to travel to Mexico and work with young people. The experience was empowering for Cari, because as she educated others about healthy living and intercultural acceptance, she learned about herself and grew as a person. Cari is pursuing a degree in Early Childhood Education at Wheelock and hopes to manage her own child care center one day.
Amarielis "Amy" Morales
Amy graduated from Boston Community Leadership Academy, a pilot high school in the Boston Public Schools System. Her passion is helping families in need. She strives to create opportunities for all members of her community, including children, adults, and animals. During high school, Amy volunteered in homeless shelters and animal shelters, but her most meaningful service project was through the organization buildOn, which aims to build a better future for the youth of America while building schools in some of the world's poorest countries. With buildOn, Amy traveled to Senegal, stayed with a host family, and built the foundation of a school. While at Wheelock, she hopes to devote her time to early intervention reading programs for young people and would like to engage in service learning opportunities to other communities and countries. Amy is pursuing a degree in Juvenile Justice and Youth Advocacy.
Jazmin Wallace
Jazmin is a graduate of Taunton High School. Her passion is working with underprivileged kids and she feels that her core purpose in life is to serve others. As a high school student, Jazmin was actively involved in community service from organizing canned food drives and tutoring to volunteering at a nursing home. She was the president of her school's Key Club, a student-led service club, and used her leadership position to organize students' efforts to support several programs, including collecting donations for Toys for Tots and creating Thanksgiving baskets for families in need. Her most meaningful service experience was being involved in the American Cancer Society's Relay for Life. As a team captain, Jazmin recruited 20 students to walk and fundraise for the cancer-prevention program, and raised $5,200. At Wheelock, Jazmin is pursuing a degree in Elementary Education and hopes to become an elementary school teacher.
Class of 2017
Alexandra "Lexi" Abrecht
Lexi graduated from Chapel Hill-Chauncy Hall School in Waltham, MA, where she was a member of the National Honor Society and received the Headmaster's Book Prize for social consciousness, bravery, and leadership. At Wheelock, Lexi is double majoring in the child life dual-degree master's program and counseling psychology and minoring in autism. She is passionate about working with children and providing healthy support systems. She currently volunteers on the hematology/oncology unit at Boston Children's Hospital. Lexi is also a member of the Pi Gamma Mu chapter at Wheelock, an international honor society of the social sciences whose mission is to "encourage and promote excellence in the social sciences and to uphold the ideals of scholarship and service."
Zachary "Zach" Kerr
During his time at Methuen High School, Zach became a national spokesperson and advocate for transgender students, speaking at schools across the state. As a member of the Massachusetts Commission on LGBTQ Youth, he successfully advocated for updating the regulations in the student anti-discrimination law to include gender identity, and met with U.S. Secretary of Education Arne Duncan to discuss ways in which the DOE can support transgender students most effectively. In 2013, Zach received the Nickelodeon HALO Award for his work to support LGBT students. He was featured in Out Magazine as one of the 100 most influential people in the LGBT community. Zach recently completed a training with the U.S. Office for Civil Rights, and he serves as a Youth Advisory Board Member of the Born This Way Foundation, where he has helped young people develop new skills to enhance their advocacy and find strength in a new community of peers. He also serves as the president of Wheelock's Gender and Sexuality Alliance. Zach is studying social work and plans to continue his work to support transgender youth.
Karen Morales
Karen graduated from Fenway High School in the Boston Public Schools, where she developed her commitment to supporting others and building community. Through her church, she has helped disadvantaged communities improve their access to food and basic health screenings. She is currently one of the leaders in her youth group at church and is a Sunday school teacher who works directly with infants and toddlers. As a Wheelock student, Karen had two transformational service learning experiences with fellow students and faculty: She traveled to New Orleans to support ongoing rebuilding efforts, and she visited West Africa and worked with children in schools. Karen is passionate about child welfare and working with survivors of trauma around the globe. She is majoring in social work.
Passion for Action Alumni
Lea Bartolo '14/'15MS
Amanda Belair '14
Tamera Boggs '13
Monique Brunner '12
Laylin Chong '12/'13MSW
Ryley Copans '16
Emily Daddio '13
Jacqueline "Jacky" Elias '15
Ariel Folkerts '13
Ava Jennings '12/'15MS
Emily Jestus '15
Claire Kawski '15
Lisa Kriszun '12
Seaniece Martin '16
Carmen Piedad '16
Rachel Robohm '13
Danielle Spivack '16
Melissa Catherine Tineo '15
Lyonel Traversiere '14
For information about the Wheelock College Passion for Action Scholarship Program or the Passion for Action Leadership Award Dinner, please contact Stephen Muzrall, Director of Major Gifts & Planned Giving, at 617-879-2266 or email@example.com.


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Monktail at Wikia
Welcome to the Monktail mini wiki at Scratchpad!
You can use the box below to create new pages for this mini-wiki. Make sure you type
[[Category:Monktail]] on the page before you save it to make it part of the Monktail wiki (preload can be enabled to automate this task, by clicking this link and saving that page. Afterwards, you may need to purge this page, if you still see this message).
The Monktail Creative Music Concern is a collective of composers, musicians and artists based in Seattle, WA who thrive on the atypical and exigent; the real weirdo stuff.
The MCMC exists to provide, propagate, promote, produce and preserve creative / experimental / exploratory music and associated art forms in its community through public performance, public and private educational forums, broadcasts, musical and visual recordings, and any other means of documentation appropriate and/or available at any time.
The folks involved get together under different aliases contingent on their predilection within any situation that permits them to exist. Groups range from 2 to 10 players generally, and have free improvisation/spontaneous composition as their underlying principle. Curating these specialized ensembles presents the group members with the unique opportunity to improvise in many different contexts as a performance as one particular unit organically mutates into another, offering the listener the unparalleled, or at least amusing, experience of live, acoustic, sonic gymnastic hallucinations. It's a gas. www.monktail.com


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Index >> Games & Entertainment >> Tools & Editors >> Joyvy Game Center >> Download
Report SpywareJoyvy Game Center 1.3.0
Read user reviews Submit a review Check last version on developer's site
Download Links:
Click the file link below to download Joyvy Game Center 1.3.0 from software publisher's official website. You can right-click the file link and select "Save link as..." menu to download this file to a specified directory on your computer.
Warning: The download URL links to a file which may be missing, deleted or redirected to other file. It's not recommended to download it.


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UK Finishes Sixth at SEC Championship, Second in First Session
March 22, 2014
Complete Results | Photo Gallery | Interviews | Championship Central
|#12 Auburn
|#15 Arkansas
BIRMINGHAM, Ala. - The University of Kentucky gymnastic team scored a 195.075 to finish second in the first session and sixth overall at the 2014 SEC Gymnastics Championship Saturday at the BJCC Arena in Birmingham, Ala.
UK finished behind No. 12 Auburn's 195.550 and ahead of No. 15 Arkansas' 194.900 and Missouri's 194.650 in the first session. No. 4 Alabama won the meet with a 197.875, followed by No. 1 Florida with a 197.700. Among the eight teams, Kentucky finished sixth overall. It is the Wildcats' highest finish since 2012 when they placed fifth out of seven.
"I thought we had a pretty solid day on three events," UK head coach Tim Garrison said. "We ended up where I thought we would if that were to happen. Starting well on beam was critical. They did great, start to finish, all six went up and did well. After some struggles on floor, they found a way to come back on vault, it was a mindset to let go of the last event. They performed well on vault to set us up for bars, the last event. It was a good rotation to finish on, I was pleased to see them handle it so well."
Senior Audrey Harrison, redshirt junior Kayla Hartley and junior Shannon Mitchell earned All-SEC honors. Harrison earned her first career honors thanks to a 39.125 in the all-around, the second-highest total in the first session. Hartley was named All-SEC for the second time in her career after a 9.850 on uneven bars and Mitchell earned her first career award thanks to a 9.875 on vault along with a 9.850 on balance beam.
The Wildcats led the first session with a 49.175 on vault and a 49.050 on bars. UK notched a 48.825 on beam to open the competition, just 0.050 points shy of session-high honors. Kentucky also scored a 48.025 on floor.
Mitchell's 9.875 on vault and 9.850 on beam were both team-bests. Harrison and freshman Taylor Puryear recorded a 9.750 on floor to share team-high honors. Harrison's 39.125 all-around score and Hartley's 9.850 on bars also led the Wildcats.
For the first time this season, the Wildcats started on beam. They were looking for a big start on their weakest event, and that's exactly what they got. A 48.825, their second-highest total of the year on the apparatus, put UK in first place after the first rotation.
Along with Mitchell's 9.850, Harrison and fellow senior Kayla Sienkowski each posted a 9.775. Freshman Taylor Puryear registered a career-high 9.725 and junior Sara Shipley added a 9.700 in her first appearance of the season.
UK then moved to floor, where it scored a 48.025. Harrison and Puryear led the Wildcats with a 9.750, while Hartley recorded a 9.700. Juniors Shelby Hilton and Kenzie Hedges scored a 9.450 and a 9.375, respectively.
Kentucky found itself in fourth place after two events, but a session-high 49.175 on vault quickly turned things in UK's favor. The score, UK's third-highest of the year on the event, put the Wildcats in a tie for second after three events.
UK's vault routine was led by Mitchell's 9.875, followed closely by a 9.850 from both Hartley and senior Holly Cunningham. Harrison and Hedges each added a 9.800.
After three events, Auburn was in first, but just 0.050 points separated UK, Mizzou and Arkansas for second place. Tied for second with MU, the Wildcats finished the meet with a 49.050 on bars to close the gap with Auburn and ensure Mizzou and Arkansas would not catch them.
The 49.050 was UK's second-highest of the season on the apparatus, led by Hartley's 9.850. Sophomore Amy Roemelle and Sienkowski both scored a 9.825 and Harrison scored a 9.800. Sophomore Montana Whittle anchored the day for the Wildcats with a 9.750.
The Wildcats are expected to make their 10th consecutive NCAA Regionals appearance. The NCAA Selection Show will be held on March 24, when UK will find out its postseason destination.
Regionals are held on April 5 at one of six schools. The 2014 hosts are Arkansas, Georgia, LSU, Minnesota, Penn State and Washington. The NCAA Finals, in Birmingham, Ala., will be from April 18-20.
Follow Championship Central for a behind-the-scenes look at the SEC Championship and continuing to the NCAA Regionals . Breaking news, behind-the-scenes updates, photos and more can be found on Twitter at @UKGymnastics and Facebook at Facebook.com/UKGymnastics.


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PHASE3™ passionately research, develop and create leading edge online marketing, and printed communications that we apply to achieve stronger customer connections for our clients' long-term success.
PHASE3 Strategic Creative are a team of creative and strategic thinkers who set about building and managing smart marketing communications across a range of digital, web and print media. Our core services are Branding, Design & UX, Digital Marketing and Web Design & Development.
Call Brett now (09) 360 0851 for a free 50min Consultation.
PHASE3 offer a range of customised digital solutions from eCommerce, Content Management Systems, Integration and Automation in support of your business requirements and long-term growth.
PHASE3 websites are content managed capable, meaning you can easily update or add content information, words and pictures from your own computer - at any time.
We have a range of market ready online shopping solutions in support of your unique requirements. Talk to us about integrating your back-end stock and online sales systems.
We recognise everybody has different needs and budgets for marketing & web integration strategies. Talk to us about tailoring a website solution to meet your needs.
With more than 70% of website views coming from mobile devices, it is essential your website is optimized and responsive for viewing on all small screen devices.
Simplify your team's workload and improve accuracy with integrated and automated solutions customised around your business needs. Make your website work for you.
Streamline your performance reporting from one central portal. Display all your website activity with custom widgets and analytics - display multiple-sites and real time activity - in one view.
When you're ready to realise your online marketing vision. PHASE3 will help you design your own integrated online campaign to drive more customers to your business and products.
Create effective online marketing and integrated solutions that engage directly with your targeted customers to promote sign-up and sales conversion.
Know where your online dollars are most effective. Our in depth reporting allows us to continually refine online marketing strategies and ROI.
Take advantage of social media and improve your brand awareness and loyalty. Reach your customers through inventive social-media marketing campaigns.
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Let us make your life easier by providing concise and real time campaign reporting - stay in control and optimize your ad spend on what actually works for your business.
Let us show you how to implement effective and engaging lead generation tools to convert your leads into long-term customers.
PHASE3 have a strong history in brand naming and creation. From Boutique to National Brands. Understanding Brand Personality is Key to long term brand solution and Market Launch success.
Smart brand strategies and awesome graphic design go hand in hand. They encapsulate and communicate the core values and personality of your company, products and people.
Ensure your brand message is cohesive across all media and connects strongly with all customers and stakeholders.
Name it once, name it right. You only get one chance to make a first impression. Great naming strategies ensure your brand and business is clearly understood by your target market.
Understanding of the importance of great design and how this integrates with modern printing techniques. We remain committed to the art of excellence in print across all media.
Printed media provides a tactile lasting experience that your customers can always appreciate. We believe in utilising sustainable ISO certified resources wherever possible.
The adage to 'judge a book by its cover' was never more true than in the Supermarket Aisle. Make a stunning first impression with purposeful and smart package design.
Planning to attend a Trade Show? Make an impact with full site design and management solutions from PHASE3. Let us take care of the design and set installation for you.
We're ready to learn about you and your project needs. Call, email or click on 'Get a quote' and fill out the form and we'll be in touch right away.
PHASE3 is passionate about creating leading edge digital solutions to support and strive for the better long-term success of your business... it all starts with a chat, call Brett anytime - free call: 09-8467 495
Managing Director
P: (09) 360 0851 Ext. 1
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New Zealand.


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|"Daniel F. Savarese" <...@savarese.org>
|Re: [VOTE][NET] Release commons-net 1.2.0
|Wed, 28 Apr 2004 23:44:34 GMT
In message <email@example.com>, Steve Cohen writes: >Where do we stand on this? Hey, you called the vote, you're supposed to know :) >I have two +1s from known committers to the project, another +1 from Mario, >who I don't believe is a committer on this project but may be one for some >other commons projects. I believe there are three committers who haven't >voted, one newly minted, and one who I rarely see active on the list. Some votes may have popped up only on the pmc list and some only on commons-dev. By my count there are five +1s: Jeffrey Brekke Steve Cohen Mario Ivankovits Daniel Savarese Davanum Srinivas At least four are PMC members, so the vote passed. daniel --------------------------------------------------------------------- To unsubscribe, e-mail: email@example.com For additional commands, e-mail: firstname.lastname@example.com
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- Midland MA 26-L Speaker Mike (L-Type Plug, 2 Pin)
- Palm speaker/mike with volume knob and earphone jack
By buying this product you can collect up to 2 loyalty points. Your cart will total 2 points that can be converted into a voucher of 0,40 €.
Midland (U.S.A)
Show all products


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Eve Tates
I Flunked Sunday School
When Lloyd Boyd (Kevin Dean) gets a message from God to become a "personal preacher," he reluctantly begins a one-on-one ministry. Despite not really knowing what he's doing and having flunked Sunday school, Lloyd proves a beacon of hope. He saves the church he attended as a child from a hostile takeover, brings a husband (Clarence Whitmore) back into the flock and helps a millionaire (Stuart Purdy) confront his personal demons.


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The service will enable companies to track the location of their employees on a real time basis who are provided with pickup or drop facility during night shifts
Telecom service provider Tata Teleservices has launched location based services (LBS). The service will enable companies to not only track on a real time basis the location of their employees who are provided with pickup or drop facility during night shifts, but also get updates on route deviation or over speeding of the vehicle (if any).
Tata Teleservices will offer enterprise customers a host of applications leveraging its LBS Platform. These applications will broadly include: vehicle tracking services, fleet management, taxi management asset tracking services, cargo management, consignment tracking, ATM finder workforce management solutions; employee safety, sales force automation and service ticket allocation.
LBS are powered by multiple technologies which ensure a rich user experience especially for the enterprise customer segment.
In addition to the traditional GPS based navigation/tracking technology, Tata Teleservices also offers location based services powered by assisted GPS (A-GPS) and mobile network based location technology to deliver accurate tracking services.
The mobile network based location technology enables workforce management services through mobile phone devices thereby opening up a completely new market segment.
Nirmal Lifestyle will have an exclusive right to use IAAF logo and trademark for development of IAAF certified training facilities, academies and clubs in the country
Nirmal Lifestyle, one of India's leading real estate companies, has inked a 10-year licensing deal with International Association of Athletics Federations (IAAF), the governing body for athletics worldwide, to develop athletics in India and encourage greater participation in the sport.
Nirmal Lifestyle will have an exclusive right to use IAAF logo and trademark for development of IAAF certified training facilities, academies and clubs in the country. IAAF shall be providing technical and professional expertise, consultation, coaching and necessary training for establishing and running world class athletics infrastructure by way of designs, plans, specifications and technical inputs.
Nirmal Lifestyle will build the infrastructure (Training Academy) as per IAAF standards and IAAF through its development program will send coaches, lecturers and ambassador athletes like Mike Powell to train young promising athletes. This will not only contribute in improving sporting performances in India but will also positively impact India's position on the sports stage globally. Nirmal Lifestyle and IAAF will jointly work towards spreading awareness about athletics in India.
Varun Group through its overseas arm has discovered ten heavy mineral sand mining blocks covering an area of 62.5 sq km in Ankilimitraha, Belay and Analapatsy areas in Madagascar
Varun Industries has evolved from being India's leading steelware exporter to a highly proactive multi-dimensional global conglomerate with business interests that include steelware, agri products, oil and natural gas, wind energy, uranium, heavy mineral sand, gold mining, gems and jewellery.
Varun Group through its overseas arm has discovered ten heavy mineral sand (HMS) mining blocks covering an area of 62.5 sq km in Ankilimitraha, Belay and Analapatsy areas of Anosy region in Madagascar.
The total heavy mineral (THM) resources in these permit areas is about 266.8 million tons which contains 221 million tons of ilmenite, 1.84 million tons of rutile, 1.84 million tons of leucoxene, 9.2 million tons of monazite, 18.4 million tons zircon, 9.2 million tons of garnet, 2.76 million tons sillimanite and 9.2 million tons of spinel.
On Tuesday, Varun Industries ended 1.17% up at Rs225.10 on the Bombay Stock Exchange, while the benchmark Sensex declined 0.37% to 18,744.56.


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Taylor Nichols
Jurassic Park III
In need of funds for research, Dr. Alan Grant (Sam Neill) accepts a large sum of money to accompany Paul and Amanda Kirby (William H. Macy and Tea Leoni) on an aerial tour of the infamous Isla Sorna. It isn't long before all hell breaks loose and the stranded wayfarers must fight for survival as a host of new -- and even more deadly -- dinosaurs try to make snacks of them. Laura Dern, Michael Jeter, Alessandro Nivola and Trevor Morgan co-star.


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Mark Haffner on Nov 10, 2015
I never received the item. I contacted the seller and only received an auto reply. Great service, if you're looking for a phantom bow tie.
Carrie Swafford on Oct 26, 2015
I ordered this item on September 24, I just now received it on October 26. I contacted the seller October 3 asking when item would be shipped, he responded October 10 saying it would be shipped that night. October 13 was when I was notified the shipping label had been created. It wasn't until October 20, however, that the item went to the shipping facility. I originally purchased the tie for my wedding which took place on October 18. Thankfully I ordered a bow tie off another site and it made it here on time. The tie itself, once it arrived, was poorly made and it wasn't the correct color either. Will not order from this store again.
dvine95 on Oct 23, 2015
Great seller and quality product.
dvine95 on Oct 23, 2015
Great seller and quality product.
Shop policies
Our name is derived from our designer, Anh, pronounced "on." We are a lifestyle brand based in New York City. We focus on making products that are malleable and mod, suiting the aesthetic of anyone tip-toeing the fine line between eclectic taste and urban lifestyle. With each collection, we aim to have made strategically and creative products with will complete any ensemble perfectly.
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text: Comodo CA is temporarily not including free base domains/SANs in single name and wildcard SSL certificates. This means these certificate types generated with www.domain.com or *.domain.com will NOT receive domain.com as a FREE SAN. However, if you generate with domain.com, you will receive www.domain.com in the SAN field. Comodo CA is actively working on resolving this temporary system bug. The expected ETA is no later than Monday, July 24, 2017. Please contact support for any assistance.


text: Alain Moulle wrote: > Hi > > is there a rule to follow between the DLM lock_timeout > and the deadnode_timeout value ? > Meaning for example that the first one must be always lesser than > the second one ? > > And if so, could we have a deadnode_timeout=60s and the > /proc/cluster/config/dlm/lock_timeout at 70s ? or are > there some upper limits not to exceed ? The DLM's lock_timeout should always be greater than cman's deadnode_timeout. A sensible minimum is about 1.5 times the cman value, but it can go as high as you like. > Another question : > is there somewhere a list of all parameters around CS4 that > we are allowed to tune ? This isn't exactly 'documentation' but it does briefly describe the cman tunables: http://sourceware.org/git/?p=cluster.git;a=blob_plain;f=cman-kernel/src/config.c;hb=RHEL4 -- Chrissie


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1. Fee is payable at the time of admission.
2. Local students can pay the fee through pay order in the name of CMH Lahore Medical College and Institute of Dentistry.
3. Foreign students need to pay the fee mentioned in USD through banking channel as remittance from abroad.
4. Bank details for remittance from abroad can be obtained from Finance Dept.
5. Foreign students need to pay the amount mentioned in PKR here in college through pay order as per the account title mentioned above.
6. Above mentioned fee and charges are applicable to first year only.
7. The College administration does not take the responsibility of sending the reminders to Parents/Guardians for the payment of fee.
8. Detained students will be charged as per PM &DC regulations.
9. Fee refund for new admissions will be as per PM&DC regulations.
10. If foreign students do not comply with the specified tax exemption conditions then College will collect the advance tax as per prevailing rates.
11. Hostel accommodation is subject to availability.
12. Use of hostel accommodation is subject to College rules and regulations.
13. Use of electric appliances in hostel accommodation will be charged separately.
14. Use of mess and laundry is mandatory for hostilities.
15. Refund of security deposit is subject to clearance process.
16. If fee is revised by PM&DC at any time, College will charge the fee accordingly.
17. In case of any further query, please contact accounts department at 0092-42-36605550-51ext. no 134.
College follows the policy which is given by the National University of Medical Sciences/PM&DC for refund of admission and tuition dues to the students, and is subject to change as per the directions of National University of Medical Sciences and PM&DC.
Present policy is as under:-
(a) Within two weeks after the starting of classes Full fee will be refunded after deduction of admission fee.
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(c) After one month of starting classes There will be No Refund.
a. The prevailing migration policy of NUMS/PMDC will be followed.
b. 100% prevailing fee structure for the particular class along with admission fee and other allied charges will be charged irrespective of the month of migration.
c. Fee will be collected in form of pay order / demand draft only.
d. No refund will be given to outgoing Migration cases irrespective of the month of migration.
a. The prevailing policy of NUMS / PM & DC for detained students will be followed.
b. Student repeating the year shall be charged 25% less for one year only. After that full yearly fee shall be charged.
c. Fee will be collected like regular students on the due date.
d. After the announcement of supplementary result the additional tuition fee (if any) will be adjusted in the next year fee.
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All the hostelites are required to deposit refundable hostel security amounting Rs.20,000/- (Twenty thousand only) before joining. The security will be refunded after clearance.
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b. In case of joining during the academic year full dues will be charged, which will be adjusted on monthly basis.
c. Hostel room rent of final year hostelites (passing out batch) will be charged on fortnightly basis at the time of college final clearance.
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Single Room Rs 8,000/-pm
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(e) Students will not be allowed to appear in University Examination unless all dues are fully cleared prior to the University Examination.
(f) Fine and penalties are as under;
i. A fine of Rs 100/- per day will be charged if fee is paid within 31 days of due date (1st - 31st Oct).
ii. A fine of Rs 200/- per day will be charged if fee is paid after 31 days of due date (1st 30th Nov).
iii. If a student fails to pay after 61 days (without valid reason) of due date, he will be suspended from attending classes till he/she pays the dues with fine.
iv. If a student does not pay after 91 days of due date without any written permission, he/she will be suspended from the college rolls. In case of re-admission he/she will have to pay full admission fee.
v. Last date for depositing of annual dues is 30th September of every year for MBBS / BDS from 2nd year onward.
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There has been a tremendous display of racism so far at the 2012 Republican National Convention, and that's just within MSNBC, which did a Stalinist purge of GOP minority speakers:
One of the left's favorite attacks on the Republican Party is that it is the party of old white people, devoid of diversity and probably racist.
If you were watching MSNBC's coverage of the Republican National Convention in Tampa on Tuesday night, you might believe those assertions, since missing from the coverage was nearly every ethnic minority that spoke during Tuesday's festivities.
In lieu of airing speeches from former Democratic Rep. Artur Davis, a black American; Mia Love, a black candidate for the U.S. House of Representatives from Utah; and Texas senatorial hopeful Ted Cruz, a Latino American, MSNBC opted to show commentary anchored by Rachel Maddow from Rev. Al Sharpton, Ed Schultz, Chris Matthews, Chris Hayes and Steve Schmidt.
Only South Carolina Governor Nikki Haley, a Sikh Indian-American, did not get "whitewashed" by MSNBC's clannish producers, presumably because she "passed for white."
The media hatred of Republicans wasn't limited to MSNBC by any stretch, with Yahoo! Washington Bureau Chief David Chalian caught saying that Mitt Romney would be "happy to have a party when black people drown" in reference to Hurricane Issac making landfall in Louisiana.
Chalian was since fired by a backpedaling Yahoo!, but the disturbing truth remained that he felt comfortable making such a comment among his fellow partisan newsmen, and that at least one of them could be heard to laugh in response.
When there weren't minorities to "disappear" or be used in grotesque imagery, liberal reporters too it upon themselves to simply make things up, as Harper's Jack Hitt did, inventing racism where he expected it to be.
The Mainstream Media's willingness to manufacture racism in support of their allegiance with their fellow Stalinists in the Democratic Party never ceased to amaze me. That they're willing to make their racism so obvious tells me just how far their feigned standards have fallen.


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Thomas Müller <thomas.muel...@day.com>
|Re: [jr3] Store journal as nodes
|Fri, 12 Mar 2010 12:53:16 GMT
Hi, > In case of cluster db journal, the hostname of db connection. The hostname of the database (if a database is used) and the database name needs to be known when creating the repository object. Storing it in a 'repository.xml' file is possible, but it's just an unnecessary indirection. If you keep this information in the repository.xml file, where do you store the path of the repository.xml file? If the user name and password need to be protected (not stored as plain text) how do you do that? Using yet another indirection (JNDI)? I suggest to pass the database URL (or whatever storage you use) when creating the repository object. Example (using a helper method; just an example): RepositoryFactoryImpl.openRepository("jdbc:postgresql:repo", "user", "password"); If you want to use a repository.xml file (that only contains the database connection information) you can of course. But do you really need an XML file for the database URL, the user name, and the password? Specially if the user name and password are things that normally should not be stored in a file? Speaking about databases: do you know of a database where you need to store the location of the database files in an XML file? I guess there are some databases where you *can* do that, but I don't know any where you *have to*. >>> Configuration should be editable without boot the repository. >> Why? > Again, for db store, if db host changes after repository shutdown, we > should be able to config the repository to use a different db host. > Like we can change in current repository.xml. The current repository.xml file contains much more than just the database connection settings. It contains the search index configuration (or at least part of it), file system configuration, cluster configuration, data store configuration, security configuration, workspace configuration (for some the version store), etc. All that, except for the database connection settings, can be stored in the repository itself. Because it simplifies things. > It's a feature of some application server to manage cluster configurations. I don't see a problem here. They can. > I would prefer leave the complicity out of default standalone deployment. I like to keep things as simple as possible. The repository.xml and workspace.xml files are not required; they actually make things more complicated than necessary (specially, but not only, when clustering is used). Regards, Thomas
|View raw message


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(Mar. 10, 2009) On February 6, 2009, the Mexican consuls in Little Rock, Dallas, and Kansas City and the U.S. Immigration and Customs Enforcement Agency signed an Accord to Repatriate Mexican Nationals. (Firma de Arreglo Local de Repatriación a Nacionales Mexicanos, available in Spanish on the website of the Mexican consulate in Little Rock, http://portal.sre.gob.mx/littlerock/ (last visited Mar. 04, 2009). The accord provides measures to deport Mexican children, women, and disabled individuals safely. (Deportation Pacts Between U.S., Mexican Envoys Focus on Safety of Women, Children, Disabled, THE DALLAS MORNING NEWS, Feb. 7, 2009, available at http://www.dallasnews.com/sharedcontent/dws/news/localnews/crime/stories/DN-de
port_07met.ART.State.Edition1.4c0e810.html.) However, the text of the accord could not be located, despite comprehensive research.


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|Reviews for The Sweetest Thing
| Tamsin chapter 1 . 1/17/2014
the closest I could get
| Tamsin chapter 1 . 1/17/2014
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| Lunaris-wind chapter 1 . 10/19/2012
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| Fictionators chapter 1 . 5/9/2012
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| Benevolent Contradiction chapter 1 . 1/8/2012
A very lovely story this is! I enjoyed reading it!
| irelandk chapter 1 . 1/5/2012
| mione03 chapter 1 . 8/26/2011
Hi,how are you?
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I hope read more fics with this shipper and others,like Rose/Bella,write by you!
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| Taiki chapter 1 . 8/26/2011
This was an excellent story and as a one-shot it is great and makes one want to beg for more of it, even knowing adding more may ruin it. At least for me that is. More stories like it please for you or from your co-writer. Loved it.
| HeartOfDarkess chapter 1 . 3/22/2011
aww hun that was a great combo of sweet and hot! great oneshot! xx Lisa.
| Robrator chapter 1 . 2/26/2011
oved it...and the cupcake being included ? WIN! awesome effort girls xoxo


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… This weekend I went shopping at the new Kroger Marketplace. Side note… if only Kroger thought investing in their online presence was as important as their retail presence. I digress. The new marketplace was built across the street from the previous Kroger. One step inside and you can see why. A bakery with fresh Artisan bread, a deli…


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text: Contents[show] Links to reviews/blog posts Edit Sources/Vendors Edit Gallery Edit [insert your photos of this pattern made up] Wishlist Edit [insert your username, and make sure your preferences allow for people to contact you via e-mail. Do not list your email address here!] Retrieved from "http://vintagepatterns.wikia.com/wiki/Anne_Adams_4623?oldid=678450" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Vintage Sewing Patterns Anne Adams 1940s Suits Pleats Mail Order Inverted Pleats Skirts Jackets Welts Flaps Front button closing Topstitching Notched Collar Long Sleeves Button Trim Dart fitted Flared Skirts Gored Skirts Add category Cancel Save


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You won't be able to dismiss this by usual means (escape or click button), but you can close it programatically based on user choices or actions.
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Several neuroinvasive viruses can be used to study the mammalian nervous system. In particular, infection by pseudorabies virus (PRV), an α-herpesvirus with broad host range, reveals chains of functionally connected neurons in the nervous systems of a variety of mammals. The specificity of PRV trans-neuronal spread has been established in several systems. One attenuated strain, PRV-Bartha, causes a reduced inflammatory response and also spreads only from infected post- to pre-synaptic neurons. We review the basics of PRV tracing and then discuss new developments and novel approaches that have enabled a more detailed understanding of the architecture of the nervous system. As questions and techniques evolve in the field of neuroscience, advances in PRV tracing will certainly follow.
A time-honored protocol for tracing neural connections is to inject an enzyme or dye into a small area of the brain, from which it is taken up by nerve terminals and then transported along axons. However, most of these tracers are retained within neurons and do not diffuse to connected neurons. Those that can spread to connected neurons dilute rapidly, an obvious impediment to following multisynaptic circuits. Viruses provide a noteworthy alternative to these chemical tracers. The most useful are those neuroinvasive viruses that infect the central nervous system (CNS) after peripheral inoculation. Well known examples of neuroinvasive viruses are rabies virus (an RNA virus from the family Rhabdoviridae) and the α-herpesviruses (DNA viruses from the subfamily Herpesvirinae) [1].
When infection spreads between functionally connected neurons, it is said to be trans-neuronal. The actual mechanism of trans-neuronal spread is understood poorly. The important point is that, unlike chemical tracers, as the infection spreads, viral-encoded proteins mark each infected neuron. Consequently, neuroinvasive viruses can be self-amplifying tracers of neuronal circuitry.
In recent years, pseudorabies virus (PRV) has become the most popular α-herpesvirus for circuit-tracing studies. Despite its name, PRV has absolutely no relationship to rabies virus. The natural hosts of PRV are swine, although the virus has a broad host range; it is neuroinvasive in essentially all mammals, except higher primates. PRV can be used in a wide range of animal models but remains safe for laboratory workers. It is amenable to genetic manipulation and the insertion of large segments of foreign DNA. The 142 kb double-stranded PRV genome has been cloned into a bacterial artificial chromosome (BAC) [2], which enables easy genetic modification of the genome in bacteria using standard DNA recombination technology [3].
α-herpesviruses are pantropic; that is, they infect a variety of cell types, including neurons. Specificity of infection is mediated by a single viral protein called gD that binds to one of several receptors [4,5]. This pantropic property manifests itself in the characteristic lesions of infected tissues, such as the skin and mucosa, as well as the nervous system. Despite the capacity to infect a wide variety of cell types, PRV spreads faithfully among functionally connected neurons by direct cell–cell contact, rather than by diffusion of released virus particles through the extracellular space. These cell–cell contacts are often posited to be synapses, although evidence for this supposition is circumstantial. Despite this uncertainty, evidence that PRV spreads through defined neural circuits is strong and comes from carefully designed studies of virus transport within the nervous system (reviewed in [6]). Four main points have been established: (i) PRV infection reproduces tracing studies faithfully by well characterized, nonviral tracers; (ii) infection does not spread interaxonally to non-synaptically connected, physically adjacent circuitry; (iii) spread of PRV in the nervous system requires an intact circuit and synaptic connections; and (iv) PRV appears to spread in any neuronal circuit studied to date – that is, infection is not confined to a circuit that uses a particular neurotransmitter nor is spread confined to circuits defined by anatomy or function, such as sensory or motor circuitry. Accordingly, neurobiologists have embraced the fact that the neuron-to-neuron spread of PRV infection reports functional neuronal circuitry.
PRV neuronal infection is constrained to neurons by action of glia cells surrounding the infected neurons as well as by the local cytoarchitecture. Astrocytes and microglia respond within a few hours of neuronal infection, perhaps by detecting cytokines released by the neurons [7,8]. These activated glia can be infected, probably through glia–neuron contacts, and suppress viral replication. Although PRV infects astrocytes and brain macrophages that are in close apposition to infected neurons, infectious virions are not produced and there is no spread of PRV from infected glial cells to nearby axons outside the circuitry being traced [9,10]. These non-permissive support cells appear to form a barrier around the infected neuron [11].
Virulent strains of PRV, such as PRV-Becker, PRV-Kaplan and NIA3, are not good tracers in general. When injected at peripheral sites, virulent PRV strains induce a powerful inflammatory response that kills animals within 2–3 days of infection [12]. Although spread of the virulent virus in the nervous system can occur, injected animals die before much of the circuitry is infected. Accordingly, most PRV-tracing viruses are based on attenuated strains, such as PRV-Bartha, that replicate well but induce a substantially reduced inflammatory response. As a result, infected animals live longer, enabling deep penetration of PRV-Bartha into the nervous system. An additional attribute of PRV-Bartha is that, unlike wild-type PRV [12–15], it spreads only from post- to pre-synaptic cells in a circuit (retrograde spread only) (Figure 1). The retrograde-only spread of Bartha clearly identifies the impulse direction of neuronal circuits, facilitating circuitry analysis. This unidirectional spread phenotype of PRV-Bartha results from a deletion that removes the gE, gI and Us9 genes [16–18]. Interestingly, if the virulence of wild-type PRV is reduced without affecting the gE, gI or Us9 proteins, it is possible to achieve both anterograde and delayed retrograde spread in neuronal circuits [19].
PRV-Bartha was first exploited for defining CNS circuits that modulate the autonomic and somatic peripheral outflows [20]. Early tracing studies depended on immunohistochemistry using antibodies directed against structural PRV proteins (reviewed in [21,22]), however, recombinant viruses that expressed reporter molecules soon proved to be useful tracers. The first generation of improved PRV-Bartha recombinants expressed β-galacto-sidase or green- or red-fluorescent proteins (GFP, RFP) [23–27]. All have been used in mapping efferent neural pathways through injections of peripheral tissues (reviewed in [20]).
An important finding was that a single neuron could be infected by two different PRV strains. This dual-labeling paradigm enables neuroscientists to identify those neurons that project to two different peripheral tissues and are thus involved in orchestrating the synchronized activity of two separate afferent pathways [25,28–31]. Another important finding was that PRV-Bartha-infected neurons retain relatively normal electrophysiological properties for some time after infection [24,32–35]. Boldogkoi et al. have reported additional attenuated PRV strains that are useful for tracing [36].
PRV can map CNS circuitry directly after intracerebral injections (reviewed in [37]). Such stereotactic injections provide precise, dose-dependent infection of axon terminals. PRV virions are large, being approximately 200 nm in diameter, and have a high affinity for extracellular matrix proteins, such as heparan sulfate proteoglycan. As a result, virions do not diffuse from the site of injection. Although cell bodies at the injection site can be infected, there is more robust labeling of cell bodies that send axonal projections to the site of injection. The number of infected projecting cell bodies depends on the density of the projections at the site of injection as well as the number of infectious virions injected [38]. In all CNS injections, care must be taken to avoid the cerebral ventricles, which can lead to widespread infection of distant areas of the brain.
Precise mapping of CNS circuits requires better control of infection than can be obtained by simple stereotactic procedures. To that end, DeFalco and colleagues constructed a derivative of PRV-Bartha that replicates only when it infects a cell expressing the Cre-recombinase protein. This strain, Ba2001, does not express the viral thymidine kinase (TK) gene and therefore cannot replicate in non-mitotic cells [39] (Figure 2). When a cell expresses Cre, TK expression is activated and the virus replicates and spreads to all synaptically connected neurons. [40].
Mice lacking the melanocortin-4 receptor (MC4R) are obesity-prone when placed on a moderate-fat diet, partly owing to a defect in brown-adipose tissue (BAT) thermogenesis. BAT thermogenesis is under the control of sympathetic outflow from the brain and regulates energy expenditure and body weight. Voss-Andreae et al. used PRV-BaBlue (a PRV-Bartha expressing β-galactosidase) to trace CNS circuits from interscapular BAT in a transgenic mouse expressing enhanced green-fluorescence protein (EGFP) in MC4R neurons. Neurons expressing both β-galactosidase and EGFP were detected in several brain nuclei known to be involved in sympathetic outflow [41].
Presympathetic-premotor neurons (PSPMNs) participate in the coordination of sympathetic and somatomotor output from the brain. Many of these PSPMNs are located in the lateral hypothalamus, an area containing both melanin-concentrating hormone (MCH)-producing neurons and orexin-producing neurons. Both neurons are implicated in behaviors requiring synchronization between sympathetic and somatomotor activity. Kerman and colleagues injected sympathectomized gastrocnemius muscles and adrenal glands with either PRV-152 (EGFP-expressing) or PRV-BaBlue (β-galactosidase-expressing) tracing strains. Both sympathetic (to adrenals) and somatomotor (to muscles) brain-output pathways were labeled. Two distinct and spatially separate groups of double-labeled PSPMNs were identified in the lateral hypothalamus. These two groups also differed in the sense that they co-expressed MCH or orexin predominately [42].
PRV tracing was used to study the central innervation of apparently symmetrical ovaries and adrenal glands. The right or left organ was injected with a particular PRV-Bartha strain. In addition, the left and right organs were injected simultaneously with isogenic strains of PRV-Bartha that expressed different marker genes. The results indicated that the left ovary [43] and adrenal gland [44] received more central input than the corresponding right organ.
When PRV-Bartha is injected into the vitreous humor of one eye, infection will spread ultimately to retinal ganglion cells in the contralateral retina. In this infection paradigm, PRV-Bartha spreads into the CNS only through routes of parasympathetic and sympathetic innervation of the orbital muscles and the ciliary body, respectively. Retrograde infection by sympathetic routes leads to infection of the suprachiasmatic nuclei (SCN) and, from there, to retinal ganglion cells in the contralateral eye [14,45,46] (and reviewed in [47]). In a recent study, Viney and colleagues used this paradigm to study the local circuitry of intrinsically photosensitive retinal ganglion cells (ipRGCs). ipRGCs constitute only approximately 1–2% of all retinal ganglion cells but are of crucial importance in the control of circadian rhythm. Interestingly, the contralateral retinal ganglion cells labeled are almost exclusively ipRGCs. By injecting a low concentration of virions, the authors could identify a small number of spatially well separated ipRGCs. Through the use of a sophisticated combination of immunohistochemistry, two-photon microscopy and electrophysiology, they identified classes of ipRGCs based on their respective dendritic projections. By timing the infections carefully, spread of infection from ipRGCs to surrounding inhibitory amacrine cells could be visualized. In one experiment, they dissected the retina 3.5–4.0 days post infection and imaged the cells with two-photon microscopy. They then further incubated this retina in Ringers solution to enable the infection to progress an additional day. In this way, two time points of infection in the same tissue specimen were analyzed to demonstrate that the amacrine cells were indeed connected to the ipRGCs infected earlier. The labeled ganglion cells were functional, as evidenced by electro-physiological recordings of their response to pulses of light [34].
PRV tracing has provided insight into the neuronal inputs to luteinizing hormone-releasing hormone [LHRH; also known as gonadotropin-releasing hormone (GNRH)]-producing neurons. LHRH-neurons, a small and scattered population of cells, are an important part of a key integratory circuit that controls fertility and reproduction in mammals. In addition to its role as a neurohormone, LHRH is also believed to work as a classic neurotransmitter in brain areas known to have a role in sexual behavior. In rodents, chemosensory cues are important for the onset of puberty as well as sexual behavior and, furthermore, modulate LHRH release and neuronal activity. Classic tracing studies have identified the vomeronasal pathway consistently as a main afferent to brain areas containing LHRH-neurons and it was believed consequently that this pathway communicated the chemosensory cues to LHRH-neurons. However, Yoon et al. used Ba2001 to show that LHRH-neurons, specifically, receive input from the main olfactory epithelium (MOE) but not from the vomeronasal pathway. PRV-infected cells were found readily in the MOE, as well as in all downstream structures of the main olfactory pathway, but not in areas corresponding to the vomeronasal pathway. In a crucial control experiment, the authors performed similar injections with a non-conditional PRV-152-tracing strain to show that PRV was indeed able to infect and label both pathways [48].
The same technical approach was used to study the positive feedback of estrogen on the brain that results in luteinizing hormone release. It was not clear if estrogen acts directly on LHRH-neurons or indirectly through other neurons, nor was it clear which subtype of estrogen receptor (ER) was involved (ERα versus ERβ). Detailed investigation of LHRH-neurons is hampered by their low numbers and scattered distribution. Wintermantel et al. used genetic tools to demonstrate that ERα but not ERβ is necessary and sufficient for the positive feedback of estrogenon LHRH-neurons. LHRH-neurons only produce ERβ and thus estrogen needs to modulate their activity through other cells that produce ERα. Importantly, the authors used Ba2001 tracing to identify a group of ERα-producing neurons that send axonal projections to Cre-expressing LHRH-neurons [49].
Finally, Campbell et al. used Ba2001 and LHRH-Cre expressing mice to identify brainstem inputs to LHRH-neurons [50], further showing the utility of PRV-tracing strains in dissecting CNS circuitry.
PRV-tracing technology can quantify the dynamics of neuronal connectivity. The formation of synaptic connections in the developing brain can be observed by measuring the extent of PRV penetration into the cortex at progressive times after birth [51]. Recently, the effects of maternal separation and handling on development of the CNS were evaluated by comparing pups left with their dam [non-handled (NH)/non-separated (NS) group] with pups separated from their dam for either 15 min or 3 h [handled (H)/separated (S) groups] daily from postnatal day 1 (P1) to P8 [52]. P8 pups were injected with PRV-Bartha in the ventral wall of the stomach and spread of infection to the brainstem and cortex was analyzed 63–66 h post infection. The striatum, central amygdala and insular cortex in H/S pups had markedly less infection than did similarly infected NH/NS pups. The 15 min and 3 h H/S groups were in distinguishable. The differences in PRV spread are attributable presumably to differences in synaptic formation that occurred during the 8-day treatment period. The results also could indicate differences in either the number of synaptically connected neurons or the strength or number of synaptic connections between individual neurons.
PRV tracing provides a means of quantifying synaptic plasticity in the rat CNS [53] and peripheral nervous system (PNS) [54] in response to traumatic injury. Card et al. injected PRV into the entorhinal cortex (ERC) 30 days after traumatic injury was delivered to the ERC by controlled cortical impact (CCI). There was surprisingly little difference in the location of PRV tracing after CCI: in both sham and injured animals, PRV traced to the same brain regions. The major difference between injured and sham brains was that the total number of infected neocortical neurons was significantly greater in the injured brains. The authors suggest that time-course studies with PRV could determine whether the differences in neuronal labeling were owing to increased neuronal incorporation into the ERC circuitry or a decrease in neuronal apoptosis following traumatic injury.
Similarly, PRV tracing can be used to determine the innervation of transplanted tissue. As a potential treatment for retinal disease, Seiler et al. transplanted retinal sheets into the eyes of rats with different models of retinal degeneration [9]. To determine whether synaptic connections had formed between host and transplant tissue, PRV-Bartha was injected into the superior colliculus (SC) 2.2–9.9 months after surgery, in a region of the SC believed to receive projections from the site of the transplant. Based on spatial location of tissue and bromodeoxyuridine (BrdU) staining of host neurons, the authors identified PRV-infected neurons in the transplanted retinas of some of the rats, suggesting that PRV spread through new synaptic connections that formed between host and transplant.
PRV-Bartha spreads only from post-synaptic to pre-synaptic neurons (retrograde spread), whereas Herpes Simplex virus (HSV)-1 strain H129 appears to spread only from pre-to post-synaptic neurons (anterograde spread) [47,55]. For example, after injection into the ventral stomach wall of rats, PRV-Bartha infects projections originating from neurons in the dorsal-motor nucleus of the vagus (DMV) and then moves in a retrograde manner to the medial nucleus of the solitary tract (mNST) and to the area postrema (AP). Although HSV-H129 injected into the ventral stomach can be detected in these same CNS structures, vagal deafferentation shows that HSV-H129 reaches the mNST and AP by anterograde transport through sensory neurons projecting from the nodose ganglion. At this time, the reason for the anterograde-only spread of H129 is not understood. All attempts to date to engineer an antero-grade-only PRV strain have been unsuccessful.
A particularly useful tool in CNS mapping would be technology to identify first-order neurons that synapse directly on a molecularly defined population of neurons. Currently, monosynaptic tracing is possible using engineered rabies virus [56]. Several strategies are possible to produce similar PRV mutants. First, an essential viral gene would be deleted from PRV-Bartha. Second, transgenic mice would be constructed to express this same gene from defined promoters. Virus lacking this essential gene would replicate only in cells that supplied the viral protein. Neurons that projected to this complementing neuronal population would be infected but subsequent replication of the virus would halt because these first-order neurons lack the essential viral protein.
There is substantial interest in mapping connections to genetically defined neurons by combining conditionally replicating PRV with transgenic animals. The combination of Cre-expressing animals and Cre-conditional replicating PRV described above could soon be considered the first generation of molecularly defined tracing. Second generation molecularly defined tracing strategies will employ transgenic mice expressing a transcriptional transactivator (such as the tet-On protein) under the control of a neuron-specific promoter and a tracing virus whose replication and marker expression (such as GFP) are under the control of a tet-responsive promoter. Such a virus could initiate tracing only from a tet-On-expressing neuron. Additionally, only the tet-On-expressing neuron would have a GFP label, indicating the location of the first order neuron. Currently, tetracycline responsive systems are available for HSV and are designed for inducible lytic replication [57,58]. It remains to be seen if such systems will be developed for PRV-tracing strains.
Many investigators are now using neuroinvasive viruses as tools for tracing neural circuits. However, much remains to be done to understand the mechanisms used by α-herpes-viruses for axonal, somal and dendritic uptake; targeting to specific neurons; long distance axonal transport; and assembly and release (see Box 1). With advances in imaging, proteomics and RNA interference technology, our understanding of herpesvirus infections of the nervous system and subsequent pathogenesis will improve. These studies will provide crucial information for the construction of new genetically engineered virus strains for neural tracing, imaging and analysis of neuronal physiology. Such improved viruses would be powerful tools to elucidate brain microcircuitry, thus providing a better understanding of nervous system functions.
In this brief overview of a complex field, it was impossible to cite all the publications on α-herpesvirus neuroinvasion, neuronal infection and circuit tracing. We have resorted to selected reviews and papers to illustrate key findings as much as possible. We apologize to those colleagues whose work we did not cite. L.W.E. thanks his long time collaborator Pat Card for his continuing help, advice and encouragement. M.I.E. and L.E.P. wish to thank Jeffrey M. Friedman for his continued support. M.I.E. and L.E.P. are funded by NIH RO1 DA018799–03, with additional funding for M.I.E. from The Howard Hughes Medical Institute. This work is also supported by NIH R01 33506 and NCRR P40 RR01 18604 to L.W.E. and the Center for Behavioral Neuroscience Viral Tract Tracing Core at Georgia State University through the STC Program of the National Science Foundation under agreement No. IBN-9876754 to L.W.E. and Tim Bartness.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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|Home > Debonnair
♂ Debonnair
What does Debonnair mean?
Debonnair [debon-nair] as a name for boys is of French origin, and the meaning of Debonnair is "urbane, nonchalant". Debonnair is a variant form of Debonair (French).
VARIANT DebonnaireCREATIVE FORMS
(female) MIDDLE NAME PAIRINGS
Debonnair Stefan (D.S.), ..
How popular is Debonnair?
Debonnair is an uncommon first name for men. Debonnair is an equivalently unique surname too for all people. (2000 U.S. DEMOGRAPHICS)
Debonnair has yet to be ranked in the Top 2000 thus far. (2015 U.S. SSA RECORDS)
Similar NamesSuggested similar names are Dennie, Dennis▼, Deodar, Deonn, Deontae, Devonn, Dewonn (see Dejuan), Donnan and Donnie▼. These names tend to be more frequently used than Debonnair.


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Aaron HowardRichmond, Victoria, AU
Versatile, well spoken with a knack for accents (South African, Irish, Northern, "Scouse" / Liverpool, "Cockney "/ London, "Brummie" / Birmingham, Cornwall, Deep Welsh etc). Can drop to Baritone for "Promo" Voiceovers. Ability with comedic slants such as spoof news reporters, and stereotypes (Camp, English Gentleman etc)
English (British), English
Middle Aged, Young Adult
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Never recorded professionally but have been experimenting with voiceovers casually for years.
High School (A - Levels UK)
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Apple Mac
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Less than one week depending on workload
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Willing to take on just about anything - specialising in comedy. Contact me with a brief of the job, and any script involved. I'll record a sample (complete with music if desired - charge extra) and if you like what you hear - we'll talk price, turnaround and any other details.
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