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Development and evaluation of new mask protocols for gene expression profiling in humans and chimpanzees
Cross-species gene expression analyses using oligonucleotide microarrays designed to evaluate a single species can provide spurious results due to mismatches between the interrogated transcriptome and arrayed probes. Based on the most recent human and chimpanzee genome assemblies, we developed updated and accessible probe masking methods that allow human Affymetrix oligonucleotide microarrays to be used for robust genome-wide expression analyses in both species. In this process, only data from oligonucleotide probes predicted to have robust hybridization sensitivity and specificity for both transcriptomes are retained for analysis.
To characterize the utility of this resource, we applied our mask protocols to existing expression data from brains, livers, hearts, testes, and kidneys derived from both species and determined the effects probe numbers have on expression scores of specific transcripts. In all five tissues, probe sets with decreasing numbers of probes showed non-linear trends towards increased variation in expression scores. The relationships between expression variation and probe number in brain data closely matched those observed in simulated expression data sets subjected to random probe masking. However, there is evidence that additional factors affect the observed relationships between gene expression scores and probe number in tissues such as liver and kidney. In parallel, we observed that decreasing the number of probes within probe sets lead to linear increases in both gained and lost inferences of differential cross-species expression in all five tissues, which will affect the interpretation of expression data subject to masking.
We introduce a readily implemented and updated resource for human and chimpanzee transcriptome analysis through a commonly used microarray platform. Based on empirical observations derived from the analysis of five distinct data sets, we provide novel guidelines for the interpretation of masked data that take the number of probes present in a given probe set into consideration. These guidelines are applicable to other customized applications that involve masking data from specific subsets of probes.
The development of gene expression microarray technology over a decade ago has revolutionized the analysis of the transcriptomes from numerous organisms. The earliest gene expression microarrays focused on widely-used experimental organisms, such as
In such cases, gene expression is often conducted using microarrays designed to evaluate a closely-related species or organism (reviewed in ref.
One approach to address this problem is to remove (mask) data from probes predicted to have poor affinity for orthologous transcripts based on sequence information (reviewed in ref.
Here, we developed updated mask protocols for the analysis of human and chimpanzee gene profiles with commonly used Affymetrix human oligonucleotide microarrays. We first describe the development of new mask files which only retain data from probes that are perfectly matched to a single human and single chimpanzee genomic sequence. Next, we apply these masks to an existing publicly available oligonucleotide microarray gene expression data set representing five tissues derived from six humans and five chimpanzees
Properties of individual probes
We developed an algorithm to rapidly map short sequence tags to complete genomes (Renaud and Wolfsberg, unpublished) and used it to determine how many times each probe in the Human Genome U133Plus2 microarray (Affymetrix) had an exact match in the human and chimpanzee genomes. The bulk of the probes (86%) in the U133Plus2 microarray have exactly one match in the human genome (Table
Table 1
Classification of probes in the Affymetrix U133Plus2 microarray
Human genome
No matches
Multiple matches
One match
Chimpanzee Genome
No match
Multiple matches
One match
Human Genome Build 36.1 and Chimpanzee Genome Build 2.1 were used in these analyses.
Figure 1
Classification of the 604,258 probes within the Affymetrix U133Plus2 microarray
Classification of the 604,258 probes within the Affymetrix U133Plus2 microarray. The pie chart depicts the relative percentage of probes comprising each of the nine probe categories described in the Methods section.
We assigned probes into nine categories based upon the number of matches (0, 1, or Multiple) with the human (H) and chimpanzee (C) genomes (Methods). These included: 0H_0C, 0H_1C, 0H_MC, 1H_0C, 1H_1C, 1H_MC, MH_0C, MH_1C, and MH_MC. The 1H_1C and MH_MC categories are among the easiest to justify. The former involves conserved single gene sequences in both species while the latter at least in part reflects multi-copy gene families in humans and chimpanzees. The 1H_0C category reflects consequences of fixed sequence differences between the human and chimpanzee genomes. Below, we will discuss possible reasons for the remaining six probe categories, with the understanding that sequence errors or polymorphisms could influence each case.
Affymetrix currently employs a system in which the annotation of each probe set is classified into one of five categories based on the evidence available for the probe set interrogating the intended gene of interest. The five categories are designated A, B, C, E, and R, with A indicating the most direct evidence for a probe/transcript relationship. Of the 358 probe sets for which 11 probes are 0H_0C (no match to the chimpanzee or human genome), 182 (~51%) were provided an A level annotation. In contrast, ~81% of the 4,648 probe sets for which 11 probes are 1H_1C (match once in both species) were provided an A-level annotation. In addition to these sequence quality issues, probes designed to overlap splice junctions absent in human genomic sequences will also fall into the 0H_0C category.
Possible explanations for the 1H_MC, MH_1C, and MH_0C probe categories include assembly errors of multi-copy genomic regions, duplication or loss of genetic material in either lineage, or mutations in duplicated segments. The classification of probes in the two remaining categories (0H_MC and 0H_1C) was unexpected since these require one or more matches in the chimpanzee and none in the human genome. This could arise due to the positioning of probe sequences across splice junctions found in human cDNA sequences. Such probes would not match the human genome; however, they could match processed pseudogene sequences present in the chimpanzee genome.
Since our downstream cross-species (i.e. human versus chimpanzee) gene expression analyses would focus on data derived from the 1H_1C probes, we next evaluated the percentage of 1H_1C probes that were located in orthologous regions of the chimpanzee and human genomes. We determined if regions are orthologous by using the liftOver tool provided by the UCSC Genome Bioinformatics Group
We then explored in further detail the 2923 1H_1C sequences that did not map to orthologous regions by liftOver. A total of 2488 of the 2923 sequences are either in an intron or an exon of a human gene, or within 5-kb upstream or downstream. Likewise, 2,021 of the 2,488 sequences were also in an intron or an exon of a chimpanzee gene, or within 5-kb upstream or downstream. Of these 2,021 genes, 1,660 are predicted to be human/chimpanzee orthologs.
Taking this additional information into consideration, we conclude that 389,704 (99.7%) of the 1H_1C probes map to orthologous regions in the chimpanzee and human genomes. This is especially impressive since the chimpanzee genome assembly used is of lower quality than the human, which would result in some probes being falsely identified as not mapping to orthologous regions in both genomes. Overall, these observations strongly support the use of 1H_1C probes for the analysis of human and chimpanzee gene expression profiles.
General properties of probe sets
A total of 91.4% of probe sets (49,957 total) in the Human Genome U133Plus2 microarray had at least one probe removed in the initial masking process (i.e. contained at least one probe not in the 1H_1C category). In addition, 3,674 probe sets were completely eliminated from the most basic masking analysis (mask0, Table
Table 2
Classification of probe sets based on number of 1H_1C probes
Minimum # of 1H_1C probes in a probe set
# of probe sets remaining
Note that 54,675 probe sets are present in the U133Plus2 microarray.
Next, we considered how many of the nine probe categories were represented in a given probe set. For each specified category, we determined the number of probe sets containing six or more probes (Fig.
Figure 2
Classification of the 54,675 probe sets within the Affymetrix U133Plus2 microarray
Classification of the 54,675 probe sets within the Affymetrix U133Plus2 microarray. The composition of probe sets with respect to probe categories is depicted. The height of each bar represents the number of probe sets (Y-axis) that contain a least one probe in the indicated category (X-axis). The gray segment of each bar represents the number of probe sets where less than six probes of the indicated category are present. The black segment comprising each bar represents the number of probe sets where more than six probes of the indicated category are present.
Effect of probe number on estimates of intra- and interspecies expression variation
Next, we sought to explore broad effects of masking on expression estimates between species. Since we are focusing on 1H_1C probes for expression estimates in both species, a major question concerns the effects of reducing the number of probes in a given probe set on gene expression scores. For this analysis, we applied mask1 to the entire gene expression set (five tissues for all humans and chimpanzees) and calculated the median interquartile range (IQR) of expression scores for all probe sets as a function of the number of remaining probes (Fig.
Figure 3
Effects of probe number on the variation of gene expression scores
Effects of probe number on the variation of gene expression scores. Median interquartile ranges (IQRs) of expression scores (Y-axes) for the indicated tissue from all humans and chimpanzees are plotted in red relative to the number of 1H_1C probes remaining in a probe set (X-axes). Median IQRs are plotted in black for simulated data wherein different numbers of probes were randomly sampled from 1H_1C_11 probe sets (see text for details). Note that only probe sets expressed in a given tissues are considered in this analysis (see Methods). Data from even (Panels A, C, E, G, and I) and odd numbers of remaining probes (Panels B, D, F, H, and J) in probe sets are presented separately due to inherent differences in the way the median polish algorithm employed by RMA processes them. Two-slope red and black lines are provided for the actual and simulated data, respectively (see Methods). The tissue from which the data was collected is indicated in each panel.
As would be expected, the median IQR decreased with an increasing number of probes. However, the relationship was not linear, with a faster decrease occurring when there were five or fewer probes than for probe sets with at least six probes measured. Adjusting for whether the number of probes was odd or even, the change in slope is statistically significant (adjusted
Additional file 1
Effects of probe number on the variation of gene expression scores. The relationships between probe number and the interquartile ranges of human gene expression scores are provided.
Click here for file
Additional file 2
Effects of probe number on the variation of gene expression scores. The relationships between probe number and the interquartile ranges of chimpanzee gene expression scores are provided.
Click here for file
Afterwards, we sought to determine if random probe masking could lead to the observed relationships between median IQR and probes remaining in probe sets. To address this question, we generated a total of nine hundred simulated masked data sets wherein we removed N1–9 probes from each of the 4,648 1H_1C_11 probe sets. Overall, this entailed generating one hundred simulated data sets for each N probes removed. We recalculated median interspecies IQRs (Fig.
A comparison of lines fit to the simulated data and real masked interspecies data (combining all human and chimpanzee data) separately, found that the estimated slopes vary for three of the five tissues studied (kidney, liver, and testis) (F-test on 2df
We sought to address the possibility that factors, such as the rates of evolution, have a substantial influence on the patterns of expression variation observed in different tissues as a function of 1H_1C probe number. For example, it has been demonstrated in the initial analysis of the current data set that patterns of differences in gene expression and gene sequences are similar in humans and chimpanzees
Additional file 3
Rates of evolution for U133Plus2 microarray probe sets based on their corresponding single RefSeq ID. The rates of evolution for U133Plus2 microarray probe sets based on their corresponding single RefSeq ID are provided.
Click here for file
Additional file 4
Relationships between numbers of probes remaining after masking and rates of evolution. The relationships between the number of 1H_1C probes in a probe set and the rates of evolution of corresponding RefSeqs are provided.
Click here for file
Effect of probe number on inferences of cross-species differential gene expression
Thereafter, we focused on quantifying the effect(s) that probe number has on inferences of differential expression between humans and chimpanzees. For each tissue, we identified 1H_1C_11 probe sets that showed differential expression across species (see Methods for details). Then, we compared the list of differentially expressed genes in a given simulated data set to the list of differentially expressed genes originally observed in the same tissue. This allowed us to calculate the median and range for each of the following: (i) overlap, (ii) gain, and (iii) loss of inferences of differential expression in the simulated data sets relative to that generated from the original 1H_1C_11 probe sets in each tissue.
Overall, we observed linear increases in both the gained and lost inferences of differential expression in relationship to decreasing numbers of probes sampled within a probe set (Fig.
Figure 4
Effects of probe number on the inferences of differential gene expression
Effects of probe number on the inferences of differential gene expression. The median number of gained (black) and lost (red) inferences of differential gene expression (Y-axes) in simulated data sets subjected to random probe masking relative to actual data are plotted against the number of probes in a probe set (X-axes) (see text for details). Error bars represent the observed range of inferred differential gene expression in the simulated data sets. Red and black data points are slightly off-set for visual clarity. Data from probe sets with even (Panels A, C, E, G, and I) and odd numbers of remaining probes (Panels B, D, F, H, and J) are presented separately, as described in Figure 3. The tissue from which the data was collected is indicated in the lower left hand corner of each panel. Note the different scales on the y-axes for heart and testes.
To illustrate the effects of masking, we compared the inferences of differential gene expression using mask5 (requiring at least 6 1H_1C probes in a probe set) and unmasked data (Additional File
Additional file 5
Comparative analysis of inferred differential expression based on masked and unmasked data sets. Venn diagrams summarizing the relationships of probe sets showing differential expression based on masked and unmasked data sets.
Click here for file
Discussion and conclusion
Strategies for cross-species gene expression analysis
Without appropriate signal processing, the analysis of multi-species gene expression data sets generated using a single oligonucleotide microarray platform can result in heavily biased inferences of differential expression (reviewed in reference
However, this computational approach leads to an additional source of error in expression measures due to the variation in the number of probes remaining in a probe set. The interpretation of masked data sets requires an
Using simulated data sets comprised of over 4,000 transcripts, we noted that the gained and lost inferences of differential gene expression increased in a linear fashion with decreasing probe number. However, the rates of change were tissue-dependent. The issue of minimal numbers of probes necessary to generate reliable expression scores has been investigated by Lu et al.
Overall, there is strong agreement between the analyses of Lu et al.
Conclusions from the empirical data
The data set of Khaitovich and colleagues
In addition, we observed that lost and gained inferences of differential expression across species increased with decreasing numbers of probes in probe sets (Fig.
Overall, we conclude that the choice of mask to apply to the human and chimpanzee gene expression data set considered in this study is highly dependent upon the general goals of the meta-analysis. Due to specialized requirements for data quantity and quality, no single mask design is optimal for all applications. Based on our median IQR analyses (Fig.
Additional applications for masks
In principle, our methods are applicable towards the development of masks for different microarray designs and other cross-species probe masking applications. For example, it is desirable to eliminate probes overlapping common polymorphisms that could affect gene expression data within a given species
Mapping of individual probe sequences to the human and chimpanzee genomes
The Human Genome U133Plus2 microarray (Affymetrix) contains 604,258 perfect match (PM) 25 mer probes organized into 54,675 probe sets which interrogate the relative abundance of ~47,000 human transcripts. For this microarray, 20,112 unique National Center for Biotechnology Information (NCBI) Entrez GeneIDs were mapped to Affymetrix probe designations using the Bioconductor R environment hgu133plus2ENTREZID in the annotation package hgu133plus2.db
Since we used the RMA (robust multi-array average) algorithm
In order to evaluate the probe sequences in the Human Genome U133Plus2 microarray, we developed a new algorithm that quickly and accurately aligns tens of thousands of short sequences to complete genomes or transcriptomes (Renaud and Wolfsberg, manuscript in preparation). This algorithm identifies all sequences with exact matches to a genome (i.e., 100% identity over 100% of the length of the query), and can also be used to identify all sequences that align with a specific number of mismatches. We used this algorithm to align each probe sequence to both strands of the human (NCBI Build 36.1) and chimpanzee genomes (Build 2 Version 1, Oct. 2005 from the Chimpanzee Sequencing and Analysis Consortium). For each probe, it was determined if its sequence matched the human genome zero (0H), one (1H), or multiple times (MH) and whether its sequence matched the chimpanzee genome zero (0C), one (1C), or multiple times (MC) (Table
Probes were placed into nine distinct categories based on their relationship to both genomes: 0H_0C, 0H_1C, 0H_MC, 1H_0C, 1H_1C, 1H_MC, MH_0C, MH_1C, and MH_MC. Afterwards, each of the 54,675 probe sets was classified based on the abundance of probes within each of these nine categories (Fig.
Creation of mask files
The cdf environment and the probe environment were downloaded as the R packages hgu133plus2cdf and hgu133plus2probe from the Bioconductor website
Additional file 6
Instructions for the application of masks for the analysis of human and chimpanzee gene expression data obtained from Affymetrix U133Plus2 microarrays. We provide instructions for the application of masks for the analysis of human and chimpanzee data obtained from Affymetrix U133Plus2 gene expression microarrays.
Click here for file
Application of masks
The Bioconductor package affy
We generated a series of masks that only retained probes with a single match to both the human and chimpanzee genomes (1H_1C) (see Additional File
Additional file 7
Master mask file. This is the master mask file that can be applied for cross-species gene expression analyses according to the instructions provided in Additional File
Click here for file
We used the RMA algorithm
Probe number and variability of expression scores
We used the kOverA function in the Bioconductor genefilter package to select probe sets that interrogated expressed transcripts (herein defined as having a minimum expression criteria of > 100 unit gene expression score in at least four of the five chimpanzees or at least five of the six humans surveyed, unlogged data) in a given tissue of interest. The data were further partitioned so that the expressed transcripts were categorized by the number of probes remaining in their respective probe sets after the initial masking procedure. As a means of surveying variation in gene expression scores, we calculated the median interquartile range (IQR) of the selected probe set as a function of the number of remaining probes for each tissue in (i) humans alone, (ii) chimpanzees alone, and (iii) all humans and all chimpanzees. Mask1 was used for this procedure to prevent the exploration of probe sets with only one probe remaining.
The association between median IQR and number of probes is studied using linear regression. A two-slope line is fit using two predictor variables; the first predictor is the number of probes (2–11) and the second predictor takes on zero when the number of probes is less than the breakpoint and takes on the difference between the number of probes and the breakpoint when the number of probes is larger. A test of the coefficient for the second predictor variable equaling zero is a test of a change in slope for the broken line. In general, one can chose the breakpoint that maximizes the fit of the two lines in terms of variance explained and then test if the slopes for the two lines vary taking into account the multiple testing that occurs in finding the best fit. However, the limited number of probes (five even- or five odd-numbered, excluding a value of one) would not permit us to take advantage of this approach. Instead, we chose five or six as a break point depending on whether we were modeling the even numbered or odd numbered probes. When modeling all probes combined we used a breakpoint of 5.5, and used a covariate to adjust for whether the observation was for a probe set with an even or odd number of probes. The breakpoints used are supported by results from previous papers studying the association between IQR and number of probes
Probe number and inferences of differential expression
To quantify the effects that the number of probes within a probe set have on inferences of differential expression, we first calculated gene expression scores in all fifty-five .cel files using the mask1 procedure. Afterwards, we identified genes that were differentially expressed across species (herein defined as having a 1.2-fold change, Bayes moderated t-test
Next, we generated simulated mask1 files that included all the probes listed in the mask1 file with additional masking of X probes (ranging from one to nine) randomly selected from each 1H_1C_11 probe set. We generated one hundred simulated mask1 files for each value of X. Each of these nine hundred masked data sets was considered in the downstream analysis of gene expression scores for the fifty-five .cel files.
Probe number and rates of evolution
Rates of nucleotide substitution on the human and chimpanzee lineages were calculated using PAML v. 3.15
Authors' contributions
DMT carried out the development and application of the masks, characterized the properties of masked expression data, and helped to draft the manuscript. GR and TGW developed the mapping algorithms, conducted the analyses necessary for assigning the number of times the oligonucleotide probes matched the human and chimpanzee genomes, and helped to draft the manuscript. KDS assisted in characterizing the properties of the masked expression data, provided statistical analyses, and helped to draft the manuscript. MI and DEW conducted the calculations for rates of molecular evolution. JGH conceived of the study, assisted in data analysis, and helped to draft the manuscript. All authors read and approved the final manuscript.
We thank Yoav Gilad at the University of Chicago, James MacDonald and Monica Uddin at the University of Michigan for thoughtful advice and discussion. We thank Dr. Patricia Dranchak, Mallory Gerace, and Timothy Triche, Jr. at the University of Southern California for critical reading and thoughtful discussion. Computation for the work described in this paper was supported by the University of Southern California Center for High-Performance Computing and Communications
Quantitative monitoring of gene expression patterns with a complementary DNA microarray
Expression monitoring by hybridization to high-density oligonucleotide arrays
Genome-wide expression monitoring in Saccharomyces cerevisiae
Microarray analysis of Drosophila development during metamorphosis
Genomic analysis of gene expression in C. elegans
Parallel human genome analysis: microarray-based expression monitoring of 1000 genes
Cross-species microarray hybridizations: a developing tool for studying species diversity
Comparative analysis of gene-expression patterns in human and african great ape cultured fibroblasts
Intra- and interspecific variation in primate gene expression patterns
Elevated gene expression levels distinguish human from non-human primate brains
Sister grouping of chimpanzees and humans as revealed by genome-wide phylogenetic analysis of brain gene expression profiles
Regional patterns of gene expression in human and chimpanzee brains
Parallel patterns of evolution in the genomes and transcriptomes of humans and chimpanzees
Global analysis of alternative splicing differences between humans and chimpanzees
Determination of ancestral alleles for human single-nucleotide polymorphisms using high-density oligonucleotide arrays
Evolutionary sequence comparisons using high-density oligonucleotide arrays
Gene regulation in primates evolves under tissue-specific selection pressures
Multi-species microarrays reveal the effect of sequence divergence on gene expression profiles
Using DNA microarrays to study gene expression in closely related species
Versatile and open software for comparing large genomes
SeqAn an efficient, generic C++ library for sequence analysis
AffyProbeMiner: a web resource for computing or retrieving accurately redefined Affymetrix probe sets
Redefinition of affymetrix probe sets by sequence overlap with cDNA microarray probes reduces cross-platform inconsistencies in cancer-associated gene expression measurements
Evolving gene/transcript definitions significantly alter the interpretation of GeneChip data
Alternative mapping of probes to genes for Affymetrix chips
CrossChip: a system supporting comparative analysis of different generations of Affymetrix arrays
Improved precision and accuracy for microarrays using updated probe set definitions
Transcript-based redefinition of grouped oligonucleotide probe sets using AceView: high-resolution annotation for microarrays
Integrating probe-level expression changes across generations of Affymetrix arrays
Increased measurement accuracy for sequence-verified microarray probes
Conservation and evolution of gene coexpression networks in human and chimpanzee brains
Improving the sensitivity and specificity of gene expression analysis in highly related organisms through the use of electronic masks
A comparison of Affymetrix gene expression arrays
High correspondence between Affymetrix exon and standard expression arrays
Summaries of Affymetrix GeneChip probe level data
Evolutionary and biomedical insights from the rhesus macaque genome
Rate of evolution in brain-expressed genes in humans and other primates
Exploration, normalization, and summaries of high density oligonucleotide array probe level data
Preferred analysis methods for Affymetrix GeneChips revealed by a wholly defined control dataset
WebArray: an online platform for microarray data analysis
Limma: linear models for microarray data
Effect of polymorphisms within probe-target sequences on olignonucleotide microarray experiments
AffyMAPSDetector: a software tool to characterize Affymetrix GeneChip expression arrays with respect to SNPs
Novel definition files for human GeneChips based on GeneAnnot
A detailed transcript-level probe annotation reveals alternative splicing based microarray platform differences
GeneAnnot: comprehensive two-way linking between oligonucleotide array probesets and GeneCards genes
Combining gene expression data from different generations of oligonucleotide arrays
A verification protocol for the probe sequences of Affymetrix genome arrays reveals high probe accuracy for studies in mouse, human and rat
Mutational and selective effects on copy-number variants in the human genome
Bioconductor: open software development for computational biology and bioinformatics
A comparison of normalization methods for high density oligonucleotide array data based on variance and bias
NetAffx: Affymetrix probesets and annotations
Web-based GeneChip analysis system for large-scale collaborative projects
affy – analysis of Affymetrix GeneChip data at the probe level
Initial sequence of the chimpanzee genome and comparison with the human genome
Controlling the False Discovery Rate – a Practical and Powerful Approach to Multiple Testing
Linear models and empirical bayes methods for assessing differential expression in microarray experiments
PAML: a program package for phylogenetic analysis by maximum likelihood
OCPAT: an online codon-preserved alignment tool for evolutionary genomic analysis of protein coding sequences
Distinct genomic signatures of adaptation in pre- and postnatal environments during human evolution


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text: ← Part 6 ⋅ Part 8 → Y U NO LIKE MELON!? is the seventh episode of PewDiePie's Let's Play of Façade. It was uploaded on April 10th, 2012 and is 10:56 minutes long. VideoEdit 10:56 Y U NO LIKE MELON!? - FACADE - 7th Attempt full Retrieved from "http://pewdiepie.wikia.com/wiki/Fa%C3%A7ade_-_Part_7?oldid=39882" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Facade Episodes Videos Add category Cancel Save


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I wish I could put into words the loveliness of this past Wednesday morning:) I was invited to minister at a home Bible Study hosted by a couple from ...more
Report from the Mission Field- Fairfield, Tx
March 8, 2017 Comments Off on Report from the Mission Field- Fairfield, Tx
There are always many beautiful things I want to share after each trip…this one is no different. First let me share a couple of little confirmat ...more
Reflections & News as we move into 2017…
December 7, 2016 Comments Off on Reflections & News as we move into 2017…
Hi Friends, I just wanted to write a note to wish you all a very Merry Christmas. I pray so much you are soaking up every ounce of Jesus daily. He is ...more
The Release of a new "Hush Little Baby" Music Video Featuring the Refuge of Light Safe Home…
November 12, 2016 Comments Off on The Release of a new "Hush Little Baby" Music Video Featuring the Refuge of Light Safe Home…
For a few years the Lord locked hearts with my sweet friends at Refuge of Light. I not only admire their work but they are dear family to this heart o ...more
Diamond in the Rough-Release set for Oct. 3rd on HLE RADIO.COM
September 28, 2016 Comments Off on Diamond in the Rough-Release set for Oct. 3rd on HLE RADIO.COM
@ShellyWilsonDQK will be releasing her new single "Diamond in the Rough" with us on October 3rd! pic.twitter.com/1y1VhNPXs8 — HLE Radio ...more
Poetry & Ponderings
The Chosen Tool
July 22, 2017 0 comment
There is a tool you must embrace As you draw near to Christ. A tool that leaves a crimson stain A piercing of one's side. For as He molds your p ...more
It's Time To Breathe Again
July 14, 2017 0 comment
Dear Christ in all His tenderness Does come on weighted winds To nudge His sorrowed little lambs It's time to breathe again... For long it' ...more
A Moment with Jesus
July 14, 2017 0 comment
I was praying this morning and asked the Lord to give me His heart and mind….I honestly placed before Him a fear of moving in my own agenda more ...more
July 14, 2017 0 comment
We walk through our days with heads somewhat buried in the sand at that which the enemy has become so successful at. We live as if every person presen ...more
Upcoming Events
October 14, 2017
Friends of HLE Radio Concert- Dallas, TexasDallas Texas
Hush Little Baby-Featuring Refuge of Light
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Shelly Wilson Ministries International
Speaking that which the Father places deep in Shelly's heart has become to her what Ezekiel refers to as "a fire in the bones." An evangelist at heart, God has uniquely gifted her with a transparent heart for sharing her life with Christ. Little is hidden as she pours the truth in God's Word into the lives of those within hearing. Whether you are visiting her online podcast or a live event, her prayer is that all He has given her to go share will be poured out passionately in a way that reveals His loving heart of mercy and His desire for a bride made ready for His coming.
At an early age Shelly was drawn by music. When she surrendered her life to Christ and devoted all of her gifts and talents to Him, music was the first tool she used to share her testimony and life with Him. Soon He began giving her melodies and lyrics, although she does not play an instrument or read music. This became one of His most intimate ways with her and has proven one of her greatest joys. In this she is able to deliver His messages and heart cries through melodies and words that pierce the heart and soul.
What began as merely pouring out her intimate love for Christ has now become a focus of Shelly's ministry.Through ponderings, poetry, and magazine publications she shares mountains, valleys, joys, and sufferings passionately penned to share the hope of Christ, the redemption of Christ, and the restoration of Christ. As Christ continues to reveal His messages to her, He now has expanded her borders into children's books. She has learned the meaning of Ephesians 3:20, He will do immeasurably more than you could ever think or imagine..
Invite Shelly
Shelly prayerfully considers invitations to be added to her travel schedule. She delights in sharing her life with Christ with others through music, poetry, and God's Word. She has a tenderness of heart for the broken and a desire to reach the unreached with His love. For past speaking messages visit the speaker page.
Ministry Tools
Shelly's music and publications are tools to share Christ in a world that is quickly turning from Him. Her magazines have been used as tools of encouragement to many a stranger. Your purchases for yourself and friends sow a priceless seed into many lives. Become a miracle deliverer today.
Shelly ministers often in areas and places who are unable to provide travel and other expenses; Therefore, she operates as a missionary. If you would like to bless us as a partner, please click here to join her ministry mission team.


text:
Zwave HA05 Screw In Module - 300 Watts, Weather Resistant, Indoor/OutdoorItem#: Z400-1026 | Model#: HA05
Currently Out Of Stock!
Zwave HA05 Screw In Module Product Details
Manufactured by: Zwave
Warranty provided by: Zwave
UPC No: 07827508391
Mfg Part No: HA05
Box Size:
( Length:6, Width:6, Depth:6)
Shipping Weight: 1.0000 pound(s)
Click here for full warranty and support information
- Zwave logos are registered trademarks of Zwave. All others trademarks and copyrights mentioned herein are the property of their respective owners.


slug: getting-started

title: Getting Started

auth: never

body:
## Deliverable, Undeliverable, Risky, & Unknown

Every address you verify will have a **result** that is either "deliverable," "undeliverable," "risky," or "unknown." Here's what each means:
[block:parameters]
{
 "data": {
 "0-0": "**Deliverable**",
 "1-0": "**Undeliverable**",
 "2-0": "**Risky**",
 "0-1": "A deliverable result means the recipient's mail server stated the recipient exists. Kickbox has also performed additional analysis and determined this address is safe to send to within our [95% Deliverability Guarantee](doc:the-95-deliverability-guarantee).",
 "1-1": "An undeliverable result means the email address does not exist, or is syntactically incorrect (and thus does not exist).",
 "2-1": "A risky result means that the address has quality issues that may result in a bounce or low engagement. Therefore, use caution when sending to risky addresses. [Accept All](http://docs.kickbox.io/v2.0/docs/terminology#section-accept-all), [Disposable](doc:terminology#section-disposable-email-address), and [Role](doc:terminology#section-role-email-address) addresses are classified as Risky",
 "h-0": "Result",
 "h-1": "Description",
 "3-1": "Kickbox will return an unknown result when it was unable to get a response from the recipient's mail server. This often happens if the destination mail server is too slow or temporarily unavailable. In some cases, retrying your request after about 5 minutes will return a valid or invalid response. Unknown addresses don't count against your verification balance.",
 "3-0": "**Unknown**"
 },
 "cols": 2,
 "rows": 4
}
[/block]
## Result Reason

In addition to the result of a verification, Kickbox also provides the **reason** for the result. These include "invalid_email," "invalid_domain," "rejected_email," "accepted_email," "low_quality," "low_deliverability," "no_connect," "timeout," "invalid_smtp," "unavailable_smtp," and "unexpected_error":
[block:parameters]
{
 "data": {
 "0-0": "**Invalid Email**",
 "1-0": "**Invalid Domain**",
 "3-0": "**Accepted Email**",
 "4-0": "**Low Quality**",
 "5-0": "**Low Deliverability**",
 "h-0": "Reason",
 "h-1": "Description",
 "6-0": "**No Connect**",
 "7-0": "**Timeout**",
 "8-0": "**Invalid SMTP**",
 "9-0": "**Unavailable SMTP**",
 "10-0": "**Unexpected Error**",
 "0-1": "Specified email is not a valid email address syntax",
 "1-1": "Domain name does not exist or is not configured to receive email",
 "3-1": "Email address was accepted by the SMTP server",
 "4-1": "Email address has quality issues that may make it a risky or low-value address",
 "5-1": "Email address appears to be deliverable, but deliverability cannot be guaranteed",
 "2-0": "**Rejected Email**",
 "2-1": "Email address was rejected by the SMTP server, email address does not exist",
 "6-1": "Could not connect to SMTP server",
 "7-1": "SMTP session timed out or DNS query timed out",
 "8-1": "SMTP server returned an unexpected/invalid response",
 "9-1": "SMTP server was unavailable to process our request",
 "10-1": "An unexpected error has occurred"
 },
 "cols": 2,
 "rows": 11
}
[/block]
## Accept All
Some domains accept all email you to send to them, even if the email address is not valid. After an email is sent, the recipient mail server may silently discard the message or send a bounce message after the fact. In these cases, Kickbox cannot guarantee an email will be delivered to a user's inbox. Sending to an Accept All domain may increase your bounce rates and/or lower your open rates. Therefore, use a degree of caution when sending to Accept All domains.

## Did You Mean?

Kickbox may provide suggestions that you can relay on to your users, or use to attempt to sanitize a misspelled email address. The Did You Mean suggestion will pose an alternative email address when Kickbox notices a potentially mistyped address. For example, Kickbox will suggest the address 'user:::at:::gmail.com' if provided with the address 'firstname.lastname@example.com'.

 ## Disposable Email Address

Email addresses that point to domains like trashmail.com and mailinator.com are utilizing a disposable email service. These services enable users to create one-time email addresses in order to receive initial communications from a service (such as an activation email) and then are discarded. It is highly recommended you do not mail to disposable email addresses.

## Free Email Address

Kickbox identifies email addresses that are using free services such as Gmail and Yahoo! Mail. In many cases, it is perfectly acceptable to send email to a user of a free email service. However, in certain contexts, businesses can receive better open/response rates when only sending to non-free email addresses.

## Role Email Address

A role email is an address that's typically associated with a function (postmaster, support, sales, etc.) instead of a person. In some instances, mailing to a role address can lead to a decreased open rate and is generally advised against while sending marketing email.

slug: terminology

type: basic

title: Terminology


text:
I have a user that is trying to transfer a file from his desktop to a mapped network drive. He receives the attached error message. I have no other users complaining of the problem. The total path is only about sixty characters long including spaces. Any thoughts?
4 Replies
Feb 24, 2009 at 4:01 UTC
Here's the file.
Feb 25, 2009 at 7:10 UTC
We have run into similar problems before, it is not just the individual file name but the entire path that makes up the "long file name", so all the nested folders contributed to this length. Have you the user look at all the folder names along the path, some of them may need to be shortened with abbreviations, etc.
Mar 3, 2009 at 9:36 UTC
Here's the best way I've ever figured out how to do this...
Open command prompt and navigate to the path:
once you're there..at the path, type edit.exe and then close out of the edit program.
This will give you the abbreviated "short" path which may work for you.
Sep 12, 2011 at 11:22 UTC
I know this thread appears abandoned but it shows up in google search and I just had the same problem and found a resolution.
- Right-click Start, and then click Explore.
- In Windows Explorer, click Tools, and then click Folder Options.
- In the Folder Options dialog box, click the View tab.
- Under Advanced settings, click to clear the Launch folder windows in a separate process check box, and then click OK.
Just resetting the folder view options to default fixed this for us.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
Advertisement [CLICK THE BUTTON ON THE RIGHT TO CONTINUE]
Author | exchange4media News Service | Friday, Aug 18,2006 8:43 AM
Why has India, the country of innumerable brands, still not been able to establish a true global brand? This was the question that many tried to answer at the CII Marketing Summit 2006 held at the National Capital on August 17, 2006. The short-term course in the brand-building exercise did excite an enormous response from corporates.
The session saw Suhel Seth, Chairman of the Marketing Summit 2006 and Managing Partner, Counselage, and R Seshasaye, President, CII, discuss the mindset of the Indian brands and Indian consumers. Seshasaye hinted at an important challenge that most brands were fighting with – the short attention span of consumers throughout the world. "The challenge is to cast aside the Indian mindset or Indian psyche to become a total global brand," he said.
Kamal Nath, Minister of Commerce and Industry, also cast a critical eye on the Indian brands and their future. According to him, "There is a dichotomy that exists in the Indian mindset that is preventing the evolvement of Indian global brands. On the one hand, we promote Incredible India, and on the other hand we promote Credible India through its industries, the capital markets. This is the dichotomy, which pulls the Indian psyche in two different directions. But the question is: what is going to be the economic architecture in the next two decades? Is it going to be Indian values or is it going to be Indian services?"
Douglas A Baillie, CEO, HLL, talked about managing global markets in domestic markets. Giving the example of HLL's 'Surf Excel', he said, "We brought in the very best of local and the very best of global to create the global brand which has been doing well in local markets too."
The changing face of the Indian consumer was also discussed in depth at the summit. While Ajay Chowdhry, co-founder, HCL and Chairman and CEO, HCL Infosystems, believed that the changing face of the Indian consumer had brought exciting times for marketers, Vinita Bali, MD and CEO, Britannia Industries Ltd, wondered whether marketers were actually taking advantage of the changing scenario.
Sam Balsara, Chairman and Managing Director, Madison Communications, focused on the reason for the changing face of the consumers, such as demographics, easy finance, growth in the organised retail sector, media explosion and emerging sectors. However, he added that marketers had been ignoring the minute points of reaching out to consumers. "The marketers have to deliver experience, value and convenience to the consumers," he stressed.
Amitabh Kant, Joint Secretary, Ministry of Tourism, pointed out, "Building brands is all about innovating. The infrastructure and ground realities always prove a challenge. It helps when the ground realities match the projection of the brand as brand-building is not an exercise in isolation."
Among the distinguished panelists were Piyush Pandey, Executive Chairman and National Creative Director, O&M India; Prasoon Joshi, Creative Director, McCann Erickson India; Swapan Seth, CEO, Equus Red Cell; Jiggy George, Director, Cartoon Network Enterprises, India and South Asia; Arvind Rao, CEO, Onmobile; and Jessie Paul, Chief Marketing Officer, Wipro Technologies.


text:
This website was created as part of the Sunrise Primary Prevention Education and Awareness Initiative and is one piece of the Know Your Peace Public Awareness and Education Campaign to create social change by promoting healthy relationships and gender equality.
The Know Your Peace Public Awareness and Education Campaign
The Know Your Peace Campaign was developed by Sunrise of Pasco Primary Prevention Team with input from teachers and students involved with the Youth Community Action Team Project at Zephyrhills High School and other youth from the surrounding communities. The YCAT project is a school club that was developed to foster a climate of respect amongst students in school, increase awareness of healthy relationships among all students and engages each team member to become an active leader in their community. To find out more about the YCAT and how to start one at your school, contact 352-521-3358.
Sunrise of Pasco Inc. is a full service domestic and sexual violence center providing a safe,non-violent place for healing and planning a violence free life for men, women and children. Visit www.sunrise.org or call 352-521-3120, toll free 1-888-668-7273 for more information about Sunrise's services.
The development of this website was supported by the Cooperative Agreement Number 1US4CE002273-01 Revised from The Centers for Disease Control and Prevention. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the Centers for Disease Control and Prevention.
The Florida Department of Health Sexual Violence Prevention Program (SVPP) is located in the Division of Family Health Services, Bureau of Family & Community Health. The SVPP is federally funded through grants from the National Center for Injury Prevention and Control for Sexual Violence Prevention and Education and the Preventive Health and Health Services Block Grant for Sexual Assault Victim Services. State funding is provided to the Florida Council Against Sexual Violence through The Rape Crisis Program Trust Fund (RCPTF).
The SVPP supports the following activities: primary prevention educational trainings to the general public; operation of hotlines; training programs for professionals; efforts to increase awareness in underserved communities and services to victims of sexual assault through contracted providers.
Contact info for the Florida Department of Health
Sexual Violence Prevention Program
4052 Bald Cypress Way, Bin # A-13
Tallahassee, Florida 32399-1723
Telephone: (850) 245-4455
"The goal of education is the advancement of knowledge and the dissemination of truth."
— John F. Kennedy
Why we need peace now.
We need a new way of thinking about violence. We are not born violent. Violence happens when violence between people who know each other is tolerated, expected, or we make excuses for it. Read more about these troubled times.


text:
The Bass Report
Dear Friends,
Today, our campaign released a new web video, "A Better Future". We must start putting people back to work to turn this economy around and make New Hampshire, and the nation, more prosperous again. The policies supported by my opponent and her party have been tried, and failed. We as Americans deserve better than the last four years of stalled and stagnant economy. We can do better, and New Hampshire deserves better.
Please take a moment to view our video. If you agree that we must get our country back on track to a better future, click here to join our campaign.
43 Months and Counting...
The day after President Obama and the Democrats ended their convention, the American people awoke to a jobs report that delivered nothing but disappointment and further evidence that the policies supported by Annie Kuster and President Obama are just not working. We have now had 43 straight months of unemployment above 8%. Despite this, all we keep hearing from Annie Kuster and her party leaders in Washington is that we must stay on the same path, we need to try more of the same failed policies. They refuse to acknowledge that their ideas are just not working. Here is what policies supported by Annie Kuster have produced for New Hampshire and our country.
- The worst economic recovery since the Great Depression.
- Job creation that fails to meet expectations.
- Unemployment stuck above 8% for 43 straight months.
- Over 23 million Americans still struggling to find work.
- More than 360,000 Americans have dropped out of the labor force. For every job added, roughly four people stopped looking for work.
Annie Kuster's hyper-partisanship and support of more government-controlled healthcare, more failed stimulus packages, more spending and more taxes on families and small businesses will not change any of this. Charlie Bass is committed to finding real solutions to our nation's problems and working in a bipartisan way to get our economy back on track, reduce the burden on small businesses and ensure an environment in which our job creators can get back to creating jobs!
Join me in this effort and help me ensure that the policies that led us to 43 straight months of unemployment over 8% are not continued. Whether it's putting up signs, making phone calls or going door-to-door in your neighborhood, everything helps. Please click here to let us know how you can help.
From the Papers...
This past weekend, Charlie joined in "Hands Across New Hampshire", a family friendly protest in opposition to Northern Pass. The Union Leader covered the event and here's a bit of what they had to say...
"The group also recognized landowners who have not sold land top the project. Congressman Charlie Bass thanked those landowners for their financial sacrifice. Bass then told the protesters, 'I'm embarrassed we even have to meet like this.'
'We are not going to move on this until you, the people, say what and when you want it,' he promised."
Volunteer of the Week...
Meet Alli! Alli is a recent graduate of New England College, where she earned her certification to teach middle school social studies. Alli has joined us for almost every phone bank and parade! She has been a phenomenal help to us and we thank her for everything she has done and will do over the next 60 days. We are proud to name Alli as our Volunteer of the Week!


text:
Cost considerations
As with any significant purchase, it's important to understand the whole-of-system cost. Where batteries are concerned some of these can include:
- the battery
- the inverter: to convert DC to AC power for use in the home
- a battery management system: this is an electronic system that manages the safe operation of your battery by telling it when to charge and when to power your home. Some battery management systems come with an app that shows you what your battery is doing at any given time
- installation: such as the installer's labour and any siting or battery casing requirements
- wiring or retrofitting: for example to a solar system, or for use as a battery back-up system
- ongoing maintenance
- end of life recycling and/or disposal


text:
You wake up. You are naked and lying on the shadow of a palm tree in a deserted beach. The sun is high and the weather is slightly hot and as you raise yourself from the ground your head spins and you feel dizzy. You look around and see what seems to be your clothes on the base of the palm tree.
Do you:
Completion status must be 0%, 25%, 50%, 75%, or 100%!


text:
TORONTO (AP) George Karl found peace and contentment in recording a coaching milestone.
Karl earned his 1,000th coaching victory, as Al Harrington scored a season-high 31 points and Nene added 26 to help the Denver Nuggets hold off the Toronto Raptors 123-116 on Friday night.
BOX SCORE: Nuggets 123, Raptors 116
The seventh coach in NBA history with 1,000 wins, Karl also notched his 900th in Toronto, a 114-107 victory on Dec. 31, 2008. He shook hands with spectators as fans next to the Nuggets' bench bowed to him in the final seconds of the game. He was hugged by Denver's players and assistant coaches at the final buzzer.
After doing radio and television interviews on the court, Karl shared a moment of "peacefulness and contentment" with his team inside the locker room.
"I just felt together with them, I felt connected with them," Karl said. "A lot of times during an NBA season, the coach and players aren't really connected, they're fighting a lot. This was a moment, not only on the court but in the locker room afterwards, there was a great connection."
Chauncey Billups called it "a moment that you'll never forget."
"He's one of the best coaches I've played for," Billups said. "He's all about winning, he's all about playing the right way and sharing the basketball and everybody having their opportunities out there. I believe that's the way you win basketball games. Obviously, with 1,000 wins, he knows what he's doing."
Karl survived prostate cancer in 2005 and was treated for neck and throat cancer earlier this year, missing the end of last season while undergoing treatment.
"It's unbelievable," Billups said. "He's got 1,000 wins and he beat cancer twice. Those are his biggest wins."
Billups scored 21 points and Gary Forbes had 15 in place of the injured Carmelo Anthony as the Nuggets snapped a two-game losing streak. J.R. Smith scored 11 points for Denver.
Former Nugget Linas Kleiza set season highs with 26 points and 12 rebounds, and Leandro Barbosa matched his season high with 22 points, but Toronto lost its fourth straight. The Raptors have dropped six of eight.
"Our forwards couldn't stay with Harrington at all," Toronto coach Jay Triano said. "He just drove past them."
Andrea Bargnani scored 24 and Sonny Weems had 21.
Anthony missed his second straight game because of a sore right knee, with Forbes starting in his place. Anthony, the 10th-leading scorer in the NBA at 22.8 points per game, also sat out Wednesday's loss at Boston, the only other game he's missed this season.
"Without Melo, if you'd told me we were going to get 123, I would have taken that," Karl said. "It's kind of shocking that we scored that many points."
Trailing by 12 points to begin the final quarter, Toronto cut it to 111-104 on a three-pointer by Weems with 4:28 to play. Smith answered with a three for Denver, restoring the 10-point lead.
After Bargnani hit a jumper and Nene made two at the line, Kleiza cut it 116-109 by making a three-pointer with 2:18 left. After a timeout, Harrington drove for a layup but landed awkwardly and injured his left ankle. He stayed in the game.
Toronto wasn't done, and a three-pointer by Bargnani made it 120-116 with 46 seconds to go. But after a missed shot by Billups, Weems lost the ball out of bounds, giving possession back to Denver.
"I don't get worried until it's under four," Karl said. "That's my credo. Four or above, we're OK. When they had a shot and they fumbled it out of bounds, I was a little worried there."
Billups sealed it by making three of four free throws in the closing seconds.
Billups scored 15 points in the first, going 8-for-8 at the line, as Denver led 39-33 after one quarter.
Kleiza scored 17 points and grabbed eight rebounds in the second quarter, but Harrington replied with 16 points and five rebounds as Denver widened its lead, holding a 74-65 edge at the half.
Forbes scored 13 points in the third as the Nuggets led by as many as 19 before Toronto ended the quarter on a 6-0 run, cutting the deficit to 98-86 heading into the fourth.
The teams combined for 22 three-pointers, including 12 by the Nuggets.
Notes: Raptors guard Jose Calderon (left foot) did not dress, while Peja Stojakovic (left knee) missed his seventh straight game. ... Karl joins Don Nelson, Lenny Wilkens, Pat Riley, Jerry Sloan, Phil Jackson and Larry Brown as the only coaches with 1,000 wins.
Copyright 2010 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.
We've updated the Conversation Guidelines. Changes include a brief review of the moderation process and an explanation on how to use the "Report Abuse" button. Read more.


text:
Apologists love to bring up the women at the tomb. Women weren't reliable witnesses in Jewish culture at the time, so why would the gospel authors place them there if the story weren't true? But the story unravels with a little scrutiny. [Read more…]
Women at the Tomb Are Weak Evidence for the Resurrection
April 20, 2014 by 117 Comments


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Solution For Images Instead Of Radio Buttons Where The Data Can Be GraphedAsked by markashton on July 31, 2015 at 05:50 AM
Does Jotform have a solution where you can use images instead of radio buttons ... but critically where the data captured from that field can be graphed in real-time in the reports section.
I've seen the image radio button widget but this was clearly made without a solution in mind. The pictures look nice .. the widget works ... but the data captured is useless. Unless you only need 10 submissions and don't need to do any analysis whatsoever. You can't do anything with it.
Am I missing something?
Many Thanks, Regards Mark
JotForm Support
Hi Mark,
Thank you for sharing your input on this.
To confirm, the data that you are looking for the image radio button are text/numerical values? Similar to the calculation values that can be added to the radio buttons?
If that's the type of data that you would like to catch and output in your report, we could use the image radio buttons widget together with the conditional logic to output a value on the form calculation widget or on a textbox field.
In my conditional logic, here's how I set up my rules and actions:
Here's a sample form that I made: http://form.jotformpro.com/form/52114136279957. The calculation value should be something that can be added in the reports.
Is that something that would work? I hope that helps.
JotForm Support
Hi Mark,
I forgot to mention, the conditional logic will read the image labels/names setup in the image radio button widget.
So we can take advantage of this and use conditional logic to assign values. Here's how I setup one of my conditional logic, I used a text or word to output, instead of a number:
You can also choose a different input field to output the result. In my case I choose to use "Form Calculation widget" named "Calculation" in my form thinking that I might use the numerical values for calculation purposes. But you can use a textbox or other input fields if you are just outputting texts.
Let me know if that would work, and thank you for the kind words. :)


text:
Custom Built Box
Regular price
About This Product
- 01 - Field of Greens (x3), SKU: 120001A
- L2 - Jalapeno Lemonade (x1), SKU: 120020A
- L6 - Berry Lemonade (x1), SKU: 120024A
- L7 - Black Lemonade (x1), SKU: 120025A
- Skin Remedy - Beauty Shot Pack (x2), SKU: 230024A
How it works
Add 8 products to successfully build your box. Buy it one time, or subscribe for as often as you want and save when you do. Welcome to your new daily ritual.
Receive Free Shipping on your first order, and any order over $89. Otherwise, FedEx Priority Overnight shipping is calculated at checkout.


text:
Botanica Omegalicious® gives your entire family the extensively researched benefits of Omega-3, emulsified for superior absorption, great taste, and creamy texture. Made with molecularly distilled, pharmaceutical grade fish oil and all-natural flavours, this delicious formula provides 1500 mg of EPA-DHA in each Omegalicious® serving!
Directions: 1 tbsp daily
Purified Water, Fish Oil EPA & DHA Omega 3 (Sardine and Mackerel), Xylitol, Glycerin, Vitamin E, Gum Acacia, Natural Flavors and Citric Acid.
Recommended Dosage
Adults: 2 tsp per day
Kids: 1 tsp per day


text:
South China Shipbuilding Holdings (HK) Ltd. ("SCS" for short), an experienced vessel developer in Hong Kong, is founded subsequent to its exclusive acquisition of an originally Chinese state-owned shipyard (established in 1958). Combining the professional qualities of the Group Company with the shipbuilding competence of the former shipyard, SCS has become one of the most competitive and internationally-renowned modern vessel enterprises in China. For the structural frame of SCS, please refer to the "SCS Main Structural Frame Guide".
HUMMER V & VI are under construction.
KITTY FOX V, the sister ship of KITTY FOX Series, is preparing for delivery.
RAPTOR IV (DP2) of the RAPTOR Series is preparing for launching.
RAPTOR III (DP2) of the RAPTOR Series is preparing for delivery.
RAPTOR II, the sister ship of RAPTOR Series, is preparing for delivery.
FALCON I & II are sold and delivered to Indonesian client.
We have our own shipyard – South China Shipyard - in Xinhui District, Jiangmen, China.
60m MPP AHTSF VESSEL has been SOLD
Copyright © 2009 South China Shipbuilding Holdings(HK) Ltd.. All right reserved.
Powered by Smart-Info Ltd.
South China Shipbuilding Holdings (HK) Ltd.
Tel: (852) 25411282
Fax: (852) 25454540


text:
Lizz Tayler – Twice Blasted!
Released: March 25, 2014
In addition to an appearance over at our sister site, The Dick Suckers, super-vet Lizz Tayler makes her fourth stop here. All of her scenes are among member favorites, and it's easy to see why. Lizz is like a fine wine, and she only gets better the longer she's in Porn Valley. Her JOE is off the hook, and it's easy to see she's a natural born slut. The only thing left is to throw her to Mr. POV…or, maybe I should say we'll throw Mr. POV at her? Enjoy my brutha!!


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How To Cook an Egg Inside Out Yolk out White In
This video about How To Cook an Egg Inside Out, Reversing The Yolk And The White
Eggs hare laid by female animals of many different species, including birds, reptiles, amphibians, mammals, and fish, and have been eaten by humans for thousands of years.Bird and reptile eggs consist of a protective eggshell, albumen (egg white), and vitellus (egg yolk), contained within various thin membranes. The most popular choice for egg consumption are chicken eggs. Other popular choices for egg consumption are duck, quail, roe, and caviar.Egg yolks and whole eggs store significant amounts of protein and choline, and are widely used in cookery. Due to their protein content, the United States Department of Agriculture categorizes eggs as Meats within the Food Guide Pyramid. Despite the nutritional value of eggs, there are some potential health issues arising from egg quality, storage, and individual allergies.
A Japanese chef has revealed how to cook an egg inside out
In a bizarre video he shows how spinning it can reverse the colour
First, you need to tape up the egg, and then spin it in a pair of tights
Then, by boiling the egg, the yolk should appear on the outside
The reason is that the yolk is denser, so the centrifugal force moves it to the outside of the albumen when the egg is spun
The science behind it is that the yolk of the egg is more dense than the albumen.
When you spin the egg rapidly, the runny yolk is drawn towards the edge of the egg via the centrifugal force, while the albumen is pushed to the centre.
If you spin the egg for long enough, the yolk and albumen separate, so that when boiled the egg appears inside out.
If you don't spin it for long enough, the two mix so that you get an instant scrambled egg.
To get started, you'll need a pair of tights, some clear tape, a torch, ice cubes and, of course, an egg.
First, hold the torch up to the egg to check on its colour. The egg should glow. This will be important for later.
Now, get your tape and completely encapsulate the egg, ensuring that the shell will remain strong and in one piece for the next few steps.
Next, lay out your pair of tights, and wrap your egg in the middle of them. Twist it up at either end so it is secure; you may want to use small ties or bits of string to keep it in place.


text: 50 photos $1,249,830 210.22 acres 14500 Starview Ln, North Zulch, TX 77872 - Madison County Lazy M ranch Properties of this caliber do not come up often, great opportunity to own a gorgeous hunting ranch less than 30 minutes from Aggieland! Set privately at the end of Starview Ln a paved driveway winds you through the property to a 2818 sqft log home perched a-top a hill overlooking the entire ranch. The property features multiple fishing lakes, a duck slough, improved grasses, variety of mature trees, tons of elevation change and more wildlife than you can imagine. Do not miss your chance to see this ranch today. Beau Skinner or Lauren Stuart Brazos Land Company


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- Category: Hinduism
- #54356Hello,
Like other yuga Satya Yuga, Treta Yuga and Dvapara Yuga, kali yuga is one which we are living.
The time period of kal yuga is 432,000 years. We hindu believe that it is a dark yuga when Kalki avatar(10th avatar of Lord Vishnu) will appear and destroy the bad human at once.
Kal yuga had started in 23 January 3102 BC.
Editor in Review, Forum & Community
- #54377Hello
These days the the passing time of the kalyug is the childhood. According to shastras the age of the kalyug is more than 400000 years.
At the end of kalyug there will be Kaling Avtar of God as told in vedas and shastras.
With best regards
Dinesh Sood
- #54397As per the Hindu mythology, "Manvantara" is the Unit of time measurement. 16 Manvantharas makes one "Kalpa" (A day of Brahma).
Present kalpa is "Sweta varaha kalpa"
Each Manvantara is presided by a specific "Manu", apart from all deities.
Vishnu purana has the mention up to Seven Manvantaras, The first manvantara being the one ruled by "Swayambhuwamanuvu"- The First Living being on this earth created by Supreme Creator, Brahma.
The present Manvantara we live in, is the 7th manvantara Ruled by "Waivasvata manu" and Kaliyuga is included in the same, which is still in the first quarter of its operation.
As Lord Krishna enlightened Arjuna in Bhagavadgita- "Whenever Dharma or the situation or Law and order are endangered, I incarnate on to this world to destroy the evil elements, to protect the Good and to re-start the creation".
So, The present kaliyuga is believed to end with the incarnation of Lord Vishnu for the tenth time on this earth in 'Kalki Avatar" - seated on a white horse with sword brandishing like a comet. He shall come finally to destroy the bad, wicked, restore the purity of conduct in the Life of people and re-starts the creation.
- #57313kalyug for any person can end just a moment he starts doing pious works , gives up selfishness and starts doing some thing for others.It is sat,so satyug starts for the peson.
- #57888According to GOD's saying in Shreemad Bhagavad Gita, "Jab Jab dharma ki ati Glani hogi, tab ek sat dharma ki sthapan karne Bharat me main aaoonga"..
The time which mentioned is currently running as all dharma gets some what dirty and people are suffering more and more now a days. So its proper time for GOD to come on the earth to cultivate all his children from the sorrow to the world full of joy, peace and happiness which is only possible when Satyuga comes.
Hence this is very near time to end of Kalyuga and upcoming new Golden aged World called Satyuga. GOD says change your self towards positivity and fill up with virtues as soon as possible as world's end will be in nearer future.
So as per his promise, he is already on earth in India. If anyone really want to know about it and want to realise it, just visit any branch of Brahmakumaris spiritual University. They are providing all the basic knowledge of ourselves as well as GOD and its realization.
Lets together make a difference to the world by helping GOD for the task of World transformation.
Have a happy moments of realizing GOD..
Best wishes
Janak Jivani


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Gold Price Update
Economist and Hedge Fund Manager Shayne Heffernan takes a look and Gold trading live from Asia.
Spot gold rose as much as 0.4 % to $1,714.40 and was at $1,710.85 at 12:45 p.m. in Singapore. Gold fell 1.2 % yesterday, the most since July 10, to the lowestlevel in more than six weeks as the dollar gained. Gold for December delivery advanced 0.2 % to $1,712 . Silver gained as much as 1 % to $32.01 before trading at $31.945. The metal slumped to a seven-week lowof $31.5537 yesterday. Spot platinum rose for the first time in five days, adding 0.6 % to $1,581. Palladium climbed 0.8 % to$601.50, snapping a four-day decline.
Soft results statements from DuPont, United Technologies and Caterpillar have undermined stock markets, while Moody's decision to cut its ratings on regions such as Catalonia pushed the eurozone debt crisis into the spotlight.
Pressure on gold from weakness in stocks helped push prices to six-week lows at $1,705.60/oz, putting it on track to decline in October for the first month in five.
In India, historically the world's largest bullion consumer, demand picked up as prices dipped ahead of a key festival season that is seen as an auspicious time to buy gold.
Gold-backed exchange-traded funds, which issue securities backed by physical metal, saw inflows on Monday of around 112,223 ounces, with the bulk of those moving into New York's SPDR Gold Trust.
Harmony Gold Mining, South Africa's third-largest gold producer, on Tuesday issued striking miners at one of its South African mines with a dismissal ultimatum, saying the strike had already cost it 13,000 ounces in lost production.
FOMC Committee concludes a two-day policy-setting meeting.
Fifty-eight % of 60 economists surveyed said thelatest round of quantitative easing will last until late next
year or beyond after the FOMC
Emerging-market demand for bullion haspicked up, given the recent gold price decline
Imports by India, are set to climb inthe three months to Dec. 31, rising for the first quarter in six months
Holdings in gold-ETP unchanged at 2,581.9 metric tons yesterday,
Shayne Heffernan
Shayne Heffernan oversees the management of funds for institutions and high net worth individuals.
Shayne Heffernan holds a Ph.D. in Economics and brings with him over 25 years of trading experience in Asia and hands on experience in Venture Capital, he has been involved in several start ups that have seen market capitalization over $500m and 1 that reached a peak market cap of $15b. He has managed and overseen start ups in Mining, Shipping, Technology and Financial Services.Read the Terms of Service
Copyright Live Trading News All rights reserved.


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- "Nevil... please take advantage of the counselling. You do need it."
- — Marling to Nevil Estrom, after talking with him about living on Azura
Doctor Erica Marling was a molecular biologist who worked at the hidden Coalition of Ordered Governments research facilities on Azura.
Meeting NevilEdit
In Brume of 10 A.E., Marling ate with recently arrived scientist Dr. Nevil Estrom, who was having trouble adjusting to life on Azura. She suggested that he go see a counsellor to help deal with his guilt at being safe on Azura instead of back in Jacinto City, and that he should not hate himself for it. Nevil retorted that he did not hate himself, but the Coalition of Ordered Governments, along with Marling and the other scientists who had hidden on Azura and stayed safe for the past ten years. Marling noted that the facilities had been built during the Pendulum Wars, and Chairman Richard Prescott would have been crazy for not making use of it. Nevil angrily responded that some of the scientists there had been on the island since the Pendulum Wars, and noted that many of them had never even seen a Locust in the flesh. Marling pointed out that Nevil and Dr. Adam Fenix had done some of the work that kept Azura hidden by working on the Maelstrom device. Nevil told her they had no idea what it would be used for, and Marling told him that was her point. Nevil told her to shove the need-to-know bullcrap, and asked where all their food and supplies came from. Marling explained that they were self-sufficient, with a lot of agriculture on the island. He guessed that they had some nice peasants to do all that work for them, and asked if they kept out of sight like the housekeeping staff. Marling told him to spare her the lecture on theoretical socialism, and that they needed that staff to keep them supplied and cared for so they could do the research. She then explained to him that the mainland did not have the facilities they needed to develop ways to win the war, and that Adam had already set them back years by keeping the existence of the Lambent to himself. Nevil asked if anyone on Azura had seen footage from the mainland of the Locust, or if they knew about the Stranded and how the COG civilians stayed alive. Marling told him that they did get news coverage, and Nevil guessed they watched it every night while sipping a brandy to help them sleep. Marling pleaded with him again to take advantage of counselling, but Nevil angrily pushed his chair away from the table and left the cafeteria in disgust.[1]
- ↑ Gears of War: The Slab pg 132-133


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Bertha Valerius
Josephine of Leuchtenberg wearing the Swedish Cameo Tiara, by Bertha Valerius (Swedish, 1824-1895)
Porträtt av okänd kvinna med hund.
File:Bertha Valerius - Porträtt av Karl XV.jpg
Dubbelporträtt. Man och kvinna i ateljé. Herr och fru Mellgren, f. Collin. 1860-tal.
Aurora Valeria Albertina Bertha Valerius (21 January 1824, Stockholm - 24 March 1895, Stockholm), was a Swedish photographer. Valerius performed portraits by both photography and painting. In 1864 she was appointed official portrait artist of the royal court. She was awarded at the exhibition in Kungsträdgården in 1866. Bertha Valerius is represented at the Royal Swedish Academy of Arts, Uppsala Universitetsbibliotek, Royal Swedish Academy of Sciences and the Linköpings museum.
Aurora Valeria Albertina BERTHA VALERIUS 1862
Bertha Valerius , porträtt av Teresia Josephina Kreüger via Galleri Skott. Click on the image to see more!


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Mariana Sánchez Sánchez
Christian González Montaño
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The Department of the Interior plans to replace one-third of its gas and alternative-fuel vehicles with hybrids, becoming the first agency to take part in a new federal program that covers additional costs for greening up government auto fleets as opposed to buying new gas guzzlers.
Interior Secretary Sally Jewell is scheduled to announce the plan during a Wednesday news conference at a D.C. park. "We are excited to be the first to partner with [the General Services Administration] on this important initiative to make our vehicles more fuel-efficient," she said in a statement. "This is simply a good business decision that will benefit not only our bottom line but reduce our carbon emissions as well."
The General Services Administration on Monday launched a new initiative to expand the federal hybrid fleet by 10,000 vehicles and reduce the government's fuel consumption by 1 million gallons per year. Under that program, GSA has promised to pay for the incremental costs of replacing eligible automobiles with hybrids.
Interior expects to replace up to 300 vehicles from its fleet with hybrids. Sen. Ron Wyden (D-Ore.), who chairs the Senate Energy and Natural Resources Committee, called the move "a bold step" for Jewell during her first month in office.
"What better time than Earth Week and what better agency than the Department of the Interior to be the first federal agency to update its fleet with hybrid vehicles," Wyden said. "This move is good for the agency, good for the environment and sets a great example for practical ways to reduce our carbon footprint."
For more federal news, visit The Federal Eye, The Fed Page and Post Politics.
To connect with Josh Hicks, follow his Twitter feed, subscribe to his Facebook page or e-mail firstname.lastname@example.com.
E-mail firstname.lastname@example.com with news tips and other suggestions.


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|This article is about a/an monster in Tensou Sentai Goseiger.
Zaigo of the Skyfish (スカイフィッシュのザイ粉(ゴ) Sukaifisshu no Zaigo) is a mosquito-themed member of the Earth Condemnation Group Yuumajuu.
Character History
Zaigo is recruited by Kingong in a multi-stage plot: by warming humans with special fireworks, he'd force them to use air conditioners, which would ultimately use energy to hasten global warming and thus speed up the burning of Earth in the end. When he attacks a beachside resort where the Amachi family, Eri and Moune are vacationing, he ends up hitting all of them with his heat fireworks, only for the male Goseiger to arrive in the nick of time. However when Hyde uses his Presshower revealing his water summons as the only weakness he has, Zaigo hits him with the fireworks as well to prevent him from using his abilities. While a major section of the town is struck, the global warming scheme doesn't work due to it being an environmentally friendly town that minimizes all usages of air conditioners.
Seeing Hyde as the only means to defeat Zaigo as he decides to blow up the female Goseiger, Alata and Agri work with him to return him to the battlefield while keeping him cool, which turns out to be harder than any of them realize. Ultimately, the trio use a combo where Alata uses Twistornado to cool him down and Agri holds Zaigo down with Roplant while Gosei Blue transforms and uses Istop to freeze the Yuumajuu before he is sliced to bits by the Skyick Sword and Landick Axe.
After Zaigo is grown, he strikes Gosei Great with his ability before Mystic Datas Hyper appears as a fan to keep the mecha cool and Gosei Knight fuses with them into the Ground Gosei Great to finish it off.
to be added
Modus and Arsenal
- Zaigo is able to raise humans' temperature. His spark attacks are the Zaigo Stardust and the Spacy Shower.
- to be added
Behind the Scenes
- Movie Reference: Zaigo's name comes from the movie Psycho (サイコ Saiko).
- His attack on Eri and Moune while at a resort is similar to murderer Norman Bates, who kills Marion Crane at a hotel he runs.
- He is based on and named after the cryptid called "skyfish" or "flying rod". Subsequent investigations proved that they are actually moths and the appearance of skyfishes on video was an optical illusion created by the slower recording speed of the camera.
- The kanji in his personal name is the one for "Powder" (粉, Ko), which refers to how his sparks resemble.
See Also
- Skyfish - Power Rangers counterpart in Power Rangers Megaforce. See comparison page.


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For sale by Dermysam Nigeria Limited- VERIFIED SELLER VERIFIED21 Jan 1:48 pmAlimosho, Lagos
NIGERIAN USED, Mercedes-Benz, ML 320 with Cooled Air Conditioning, Roof-Rack, Electric Side-Step, Tapping Windows and Mirror, Internal Winch, Half Tinted Windows, External Winch, Fog Light, Power Steering, Sunroof, Duty Cleared at Lagos Port, Four Doors, Turbo Charged, Left Hand Drive, Cup Holder, Armrest For Comfortable Sitting, Leather Seat, Auto Transmission, Formica, Neatly Used with No Single Issue than to Pay and Drive, First Body, Accident Free, Black Colour, Well Balance on Ground, Highly Durable and Efficient to Use. Reach Seller Now To Discuss Business.
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Berlin boutique SOTO recently dropped their latest editorial and I had to share! Featuring a range of printed goodness from Milan based brand Sansovino 6 the editorial highlights soft textures and bold graphics throughout the SS15 collection.
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Lo Parkin
Freelance Illustrator & Fashion Editor at People of Print.
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Bloc Party, Foals, Disclosure, Toro y Moi and RÜFÜS are among the names revealed for Falls Festival 2015/16
Tag Archives: Hilltop Hoods
2012's event sees Sam Sparro returning home and veteran techno-dance crew Sonic Animation stepping up after a 6 year hiatus. American old-school rockers Blondie will headline.
Australia's ultimate New Year's Eve experience The Falls Music & Arts Festival is living up to its reputation by adding to the already impressive line-up.
The Falls Music & Arts Festival is the ultimate New Year's Eve festival experience, held at two beautiful Australian locations. Hot Chip, Hilltop Hoods, Bombay Bicycle Club, First Aid Kit, Maximo Park, Millions and Totally Enormous Extinct Dinosaurs have been added to the 2012 line-up.
After months of speculation, the line-up for Splendour in the Grass 2012 has arrived!
In 2011, Australia's ever impressive regional music festival Groovin The Moo played host to approx. 75,000 music enthusiasts at sold out locations all over the country.
Australia's biggest Summer music festival, the BIG DAY OUT, returns in January 2012 for its 20th anniversary. Part of the line-up announcement was made last night and capped off this morning after months of artist speculation – pretty sure Prince was rumoured to headline at one point… Here is the official BDO 2012 first round […]


text: On Mon, Jan 19, 2009 at 7:48 AM, Matej Cepl <mcepl redhat com> wrote: > On 2009-01-18, 19:16 GMT, Josef Bacik wrote: >> Sorry, I promise to not send an email everytime there is an >> update, this is just particularly important. If you are using >> an -rc2 or later kernel (which is currently whats in rawhide) >> you must use the btrfs-progs-0.18 package if you want to use >> btrfsctl, so doing snapshots or anything like that. You can >> still format/mount etc with the old btrfs-progs, its just there >> was a change to the ioctl's so any of the on the fly stuff >> won't work. Thanks, > > I have installed btrfs on my /tmp (I won't let it go further so > far ;-)), but I have still one question -- how is SELinux support > doing? I am used to use for testing purposes SELinux in the > enforcing mode. Will it work or will btrfs screw up my /tmp to > non-working state? There are patches for SELinux support but they're still being tested, probably won't be pushed till later. For now SELinux will just treat it like a volume that doesn't play properly, like FAT or anything like that. Thanks, Josef


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The original Pearl, the 8100, was the first BlackBerry to comfortably bestride the starchy business world and the pleasure-seeking mainstream. Its trim design, compact physique and newfound multimedia mojo meant you could happily flash it outside of office hours without fear of losing friends.
But if we're honest, its features were pretty tame. A basic 1.3-megapixel camera and no video capture seemed woefully sub-standard even in 2006, so we're expecting, nay, demanding, a sizeable feature step-up from its successor, the 8210.
No major surgery
There's something very familiar about the latest Pearl. Apart from a new range of paint jobs – including sunset red and Carphone Warehouse's exclusive pink – and a wavier keypad motif, these Pearlers could have been separated at birth.
Thankfully, BlackBerry creator RIM has demonstrated more imagination on the feature front, fitting the 8120 with Wi-Fi, a 2mp snapper with video capture and a 3.5mm headphone connector. On the downside, the built-in Wi-Fi only masks the fact that RIM has once again eschewed 3G. Frankly, we're bored rigid of asking when 3G will make an appearance on BlackBerry devices.
Still, while hitching a ride on the Wi-Fi express, web browsing is nippy – which only makes the plodding speed of EDGE more exasperating. Elsewhere a cursor pointer has been added for more accurate navigation, and full-fat web pages are squeezed nicely to fit the smallish screen.
Short-changed by the snapper
BlackBerry fans might welcome the new camera but the hike doesn't feel nearly enough. It's still fixed focus and offers a fickle LED flash, making picture quality vapid. True enough, video recording skills have been introduced, but again the 240x180-pixel resolution is interrupted by the dreaded judder and drag.
The addition of the 3.5mm headphone jack gives the basic player an audio lift when you plug in classy cans. And with no sound boosters, you're going to need your headphones' quality to bolster the bass. In fact, performance was surprisingly dynamic through our Sennheisers.
All your multimedia loot is stored in a microSD card. RIM has helpfully shifted the slot from under the bonnet to a hotswappable side location.
Lest we forget, the 8120 is a push emailer at its core. We've often harped on about the BlackBerry's legendary messaging prowess, so you should know by now that it's Lurch-proof to set up and use.
Rocker shocker
Typing out messages via the dual-function QWERTY keypad, however, is an altogether more thorny proposition. With two letters assigned to one button separated by a rocker switch, those with sausage fingers will struggle to discriminate.
Along with Google Maps and BlackBerry Messenger apps, serial social networkers will adore the Facebook app: they can get poked while away from the computer, upload snaps and reply to messages.
The 8120 Pearl doesn't really offer a significant advance on its predecessor. Its email talent is unquestioned, and the built in Wi-Fi welcome, but the lack of 3G and lukewarm multimedia performance is disappointing. Pearl devotees, however, will no doubt find reasons enough to upgrade.