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Mix (12+): orders of 12+ items
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Nicolo & Paradis is owned by husband and wife team David and Agnes Nicolo, a third generation grape grower in Champagne. They are located in Arsonval in the Aube Valley and their vineyards span 17 hectares across the Colombé-le-sec. The vineyards are predominantly planted with Pinot Noir, together with a little Chardonnay and Pinot Meunier for the blending process.
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The subject of this article is not named in-game.
The current title is from a guide or the game's internal data.
The Chamber[1] is a corridor in Maridia in Super Metroid. It is found further into the area, near the sections with quantities of quicksand. The room leads to the Spring Ball and as such presents an obstacle to its immediate acquisition. The middle part of the room is blocked by a wall of sand that is indestructible to Samus's weapons. The room is home to the Shaktool, a crude mechanism with the ability to penetrate this sand and "help" Samus. However, a wall of Power Bomb Blocks must be destroyed using a Power Bomb before it can destroy the sand. Any Power Bomb Samus uses will not destroy the Shaktool, as it is rather sturdy. After it has cleared the way, it can hurt Samus if she comes in contact with its rotating legs. She is free to destroy it if she wishes, or collect the Spring Ball. There are also 2 Yards on the right side of the room; interestingly enough, they can also destroy the Sand, but by the time that they are on-screen, the Shaktool has already mostly cleared the way. This indicates that the Sand is simply a special type of block that can only be destroyed if an enemy comes into contact with it.
- ^ GamePro Volume 61, page 124 (August 1994): "In the Chamber just prior to the Spring Ball, you'll encounter a strange spiked creature. Don't kill it... not yet, anyway. You'll also come upon a wall that must be destroyed with a Power Bomb. Behind the wall is another impenetrable wall. The spiked beast whose life you spared will burrow a tunnel that leads you to the Statue bearing the Spring Ball. The Spring Ball enables you to bounce when you're in the Morphed Ball state."


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Markets seem to have factored in the EU crisis. Expect flat trading from here on
The market was down today on concerns over Greece's ability to control its fiscal deficit. The Sensex ended at 17,141, down 189 points (1%) and the Nifty ended at 5,136, down 57 points (1%). The bourses plunged during the early trading session, taking cues from Asian markets, which were down as the looming concerns over the effectiveness of the eurozone bailout plans spooked investors. The market was range-bound for the rest of the day.
Asian equities slid on Tuesday on concerns over how Greece and other debt-laden eurozone countries will combat their budget deficit, ending the impressive rally in global stocks on Monday. Key benchmark indices in Hong Kong, China, Indonesia, Singapore, Japan, South Korea and Taiwan fell by 0.44% to 1.90%. US stocks racked up their biggest one-day gain in over a year on Monday as the $1-trillion emergency rescue package from the EU stemmed concerns over a new possible European credit crisis. The Dow gained 404.7 points (3.9%) to 10,785. The S&P 500 rose 49 points (4.4%) to 1,159. The Nasdaq was up 109 points (4.8%) to 2,374.
The International Monetary Fund (IMF) said that Greece's public debt is sustainable over the medium-term; however, low growth could be a setback for the country. The IMF has approved €30 billion of bailout money, along with a three-year economic program for Greece that envisages controlled spending and increase in revenue. US market regulators and six major exchanges accepted the need for new safeguards to curb trading in plunging markets, an effort to address last Thursday's mysterious market freefall.
Regulators are still in the dark over the exact cause for last week's 20-minute market plunge when many stocks dropped for several minutes before recovering most of their losses.
India's exports during March 2010 were valued at $19,908 million (Rs90,573 crore), which was 54.1% higher in dollar terms (36.9% in rupee terms) over the year-ago period. Imports during March were valued at $27,733 million (Rs126,175 crore), a growth of 67.1% in dollar terms (48.4% in rupee terms) over the year-ago period.
Foreign Institutional Investors (FIIs) were net buyers yesterday of Rs263 crore. Domestic Institutional Investors (DIIs) also bought stocks worth Rs30 crore. The rupee was down due to the weak equity markets and the dollar's gain over other currencies.
Elecon Engineering (down 1.8%) has received orders worth Rs188 crore from various companies. It will supply coal-handling equipment to GMR Energy & design and manufacture equipment for Zuberi Engineering.
Pratibha Industries (up 2.3%) has received three orders. One of the orders, valued at Rs525.97 crore, is from National Automotive Testing and R&D Infrastructure Project (NATRIP), New Delhi for construction of automotive test tracks at Pithampur, Madhya Pradesh and at Chennai, Tamil Nadu. The second order, worth Rs234.71 crore, is from the Drinking Water & Sanitation Department, Jharkhand for construction of intake well-cum-raw water pump house. The third order, valued at Rs62.13 crore, is from Indian Oil Corporation (IOC) for construction of a township project at Paradip, Orissa.
Jaihind Projects (up 3%) has received three orders. The first one is from Bangalore Water Supply & Sewerage Board for providing sewerage systems and is valued at Rs51.09 crore. The second order worth Rs7.91 crore is from GAIL (India) for laying gas pipelines at Firozabad, Uttar Pradesh. The third order worth Rs2.29 crore is from Gujarat State Petronet (GSPL) for laying natural gas pipelines.
GVK Power and Infrastructure (down 0.8%) has acquired the entire equity stake of GVK Transportation.
Shareholders of CHI Investments Ltd are unhappy over the RPG Group's decision to merge four companies with a subsidiary. They feel that the valuations done under the proposed merger are unfair
Last year, the RPG Group decided to merge four companies—Summit Securities Ltd, Brabourne Enterprises Ltd, Octav Investments Ltd and CHI Investments Ltd—with RPG Itochu Finance Ltd (RIFL), a 100% subsidiary of RPG Enterprises. However, minority shareholders of CHI Investments Ltd are not happy with the proposed merger and are raising questions over the rationale of valuations done under the proposed amalgamation.
RIFL is an unlisted firm. The new entity will be rechristened Summit Securities Ltd, which would be listed on the Bombay Stock Exchange (BSE) and the National Stock Exchange (NSE). Summit Securities Ltd (earlier known as KEC Infrastructures Ltd) was incorporated in 1945. The company is involved in design, manufacture, supply and construction of power transmission lines. Shares of CHI Investments Ltd have been suspended from trading since 3rd February due to the proposed merger.
"I am holding some shares of CHI Investments. The company is proposed to be merged with RIFL and prima facie (the move) does not appear to be fair with minority investors. I think there will be significant erosion in the value of investments in CHI Investments if the merger ratio is not changed," said an investor, preferring anonymity.
The valuations were carried out by auditing firm Grant Thornton.
The Board of directors gave their approval for a share-exchange ratio of one equity share of RIFL of Rs10 each for six equity shares of CHI of Rs10 on 10 July 2009.
In the case of Summit, one share of RIFL will be issued for every 16 shares held in Summit, while one share of RIFL will be issued for every 28 shares held in Brabourne. Reportedly, a number of minority investors believe that the valuations are unfair.
Maintaining that the company is not considering revising swap value ratio, RPG group in an email said, "The scheme has already been implemented. The question of revising swap ratio does not arise for the simple reason they are determined by independent reputed valuers and approved by the Stock Exchanges and the High Court before being implemented."
The company feels that the merger would benefit the companies by reducing overall administration costs and bring in more efficiency.
Incorporated in 1997, RIFL is engaged in hire-purchase, car leasing, commercial vehicles, construction equipment and textile machinery.
Post merger, RIFL's authorised share capital will increase to Rs180 crore from Rs30 crore.An email query sent to CHI Investments remained unanswered till the time of writing.
Asset prices for second-hand vessels in the dry bulk and tanker segments are currently going up. However, industry sources believe that players are in a wait-and- watch mode, and the rise may be a temporary phenomenon
After shipping asset prices bottomed out a few months back, a marginal rise of 8% to 10% has been witnessed in the asset prices for second-hand vessels in the tanker and the dry bulk segments. However, industry experts indicate that this could be a temporary situation, and is likely to change with the current volatility in the global economy.
The global slowdown had led to a number of cancellations for new orders placed with the shipbuilding industry. A significant drop in shipping asset prices was also witnessed last year. Asset prices in the tanker segment fell by nearly 40% from their peak in 2008. Similarly, the dry bulk segment took a hit of nearly 70% from its peak in 2008.
Now, with some signs of revival in the global economy, asset prices have moved up by around 8% to 10% from the bottomed-out rates. "Asset prices have slightly moved up, the movement is already visible with around 8% to 10% rise," said Vinay Kshirsagar, CFO, Shreyas Shipping and Logistics Ltd.
An official from Great Eastern Shipping (GE), which has always timed its asset purchases during a fall in asset prices, has also confirmed the trend. "Yes, asset prices have surely shown some improvement," said an official.
However, this revival in asset prices could very well be a temporary trend. "The revival in asset prices was expected with the economy coming back on track. Asset prices for second-hand vessels are already on a rise. However, the future trend will depend on the economy. With new issues coming up in Europe, shipping will also be affected. The industry is closely related to how the global economy performs," said SS Kulkarni, general secretary, Indian National Shipowners' Association.
"Going forward, if Chinese trading activities slow down, the entire dry bulk momentum will come to a halt. It is very difficult to predict whether the trend would continue or not. It should be a temporary phase; we expect a fall in prices. Though there may be a fall, it will not reach the levels at which prices had bottomed out," added the GE official.


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Booterstown is a charming place in the county of Dún Laoghaire–Rathdown. It is actually an Irish coastal townland as well as a civil parish and it is very close to Dublin. If you plan to visit Ireland's capital, you should definitely take into consideration spending a few days in this lovely area, especially if you are planning to have a relaxing vacation.
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Mr. Richard Chow
Los Angeles, Califo - USA
No approved endorsements
About Mr. Richard Chow
Richard is a Senior manager and innovative leader with global IT expertise and a strong record of project/line management and growing technology-driven businesses. Broad experience with all aspects of software applications deployment, technologies and business acumen. Proficient in IT system platforms, with a proven history of managing projects from spec's to implementation. Solid experience in managing vendors/teams both on- and off-shore.
Richard is a differentiator in the IT space because of his strong belief in collaboration and building partnership, as well as his ability to manage change. Additionally he often acts as the integrator pulling together cross-functional teams in a highly matrix organization.
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"Go Underneath" [video song] – Univox drum machine, Strymon El Capistan and blueSky
I recently got to borrow an old-school Univox drum machine. It's strange and unique. Where else can you get volume sliders for Quijada, Guido AND Tambourine. Not only does it have patterns for Mambo, Rumba and Bossanova, but many others including Merinque, Beguine and Habanera. What more could you want?
I felt like making some droning noise so I ran the Univox through my Strymon El Capistan and blueSky reverb pedals. I recently started working with my friends over at Strymon and am really loving what they've come up with so far. I'm looking forward to working on some more kick-ass effects with them in the future.
I decided to turn this drum machine effects noise fest into a more involved piece, so I took the original unaltered drum/effects track and added piano, guitar, drums, vocals and some synths. For the video I decided to follow jack conte's "video song" format, where:
1. What you see is what you hear (no lip-syncing for instruments or voice).
2. If you hear it, at some point you see it (no hidden sounds).
Hope you like it.
3 Comments
Glen SuttonOctober 8, 2010
simply awesome Ethan. Reminds me of Eno's work.
stateshirtOctober 11, 2010
Thanks a lot!! 🙂
snorb!November 12, 2010
Jesus… this is so good.


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A Food & Wine Top Five Professional Wine School
The International Wine Guild Is One Of The Top Five Professional Wine Schools In The US For Professional Wine Education. + click here for more
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Professional programs of the International Wine & Spirits Guild are approved and regulated by the Colorado Department of Higher Education, Private Occupational School Board. We are a state approved wine school.
Have you ever wanted more from your wine appreciation? Wanted to dig deeper, become more knowledgeable and possibly an expert of wine? Growing into a wine aficionado requires a love of wine and its history, a desire to know more and a true acquired sense of the subtleties of wine tasting. Whether you are a connoisseur or are a beginner, there are wine schools and sommelier courses that can elevate you to the professional status of wine aficionado. Wine classes are just the beginning of a lifetime of appreciation and professional wine study.
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The International Wine & Spirits Guild was established to encourage study to develop technical and professional understanding and increased knowledge of wine throughout all areas of the wine and food service industry.
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In addition, if you are a wine educator, or would like to develop the skills to teach others about wine, there is also an advanced program path leading to a Guild Wine Master Educator.
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Plesk utilizes Suexec as a CGI wrapper for security. The suexec wrapper prevents the CGI script from having permissions set over 755. Also the script should have owner and group specified by SuexecUserGroup directive for the appropriate domain. In Plesk this user is the FTP user for current domain and the group is always 'psacln'.
You can read more about suexec at: http://httpd.apache.org/docs/suexec.html#model


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Full face lift after 2 weeks? (Photo)
Doctor Answers 7
Full face lift after 2 weeks?
Thank you for your question.
Recovery and Results :
After the face lifting operation the recovery period depends on your physical status, age, general health and condition mainly. The operation itself does not take too long therefore you receive a mild anesthesia. Since the anesthesia that you receive is mild; you will not have problems associated with anesthetic agents after the operation. This is especially important and can be achieved with only a team of plastic surgeons like we have. After the operation you will have stiches and bandages which will be removed after the 10th day. You can start to do your daily activities just after the operations but we do not advise you heavy lifting, running or activities that require excess physical activity. The more you rest, the faster your recovery will be.
Learn more: http://www.estetikinternational.com/face-lifting-operation/
Learn more: http://www.thomassenplasticsurgery.com
The right technique by the right surgeon, and clear communication, are vital to achieve a beautiful result
In comparing your photos, the wrinkling pattern in your lower face and jawline indicates minimal improvement. Assuming that you're not purposely frowning in the "after" photo (which would accentuate the wrinkling), this lack of improvement is due to the wrong surgical lift technique being used. For example, a mini-face lift, which addresses the outer regions of your face, or a "skin only" lift, which places tension on skin only rather than on the deep muscle [called "SMAS"] layer and results in recurrent sagging, may have been used. I find that a mini-face lift is not a very useful technique in your age group because of the presence of deep folds and wrinkles around the mouth, nasolabial folds and neck, which wouldn't be touched by the "mini" technique.
Perhaps the surgeon did not know to address the areas of wrinkling which caused him/her to select a technique that turned out to be inappropriate for your true concerns? If so, then you had one idea in mind, and your surgeon had another - you weren't on the same page which is most often caused by inadequate communication between you and your surgeon. However, if your surgeon DID intend to address the areas of wrinkling and nonetheless selected one of these techniques, then inexperience likely plays a role. If your surgeon did surgically lift the areas of wrinkling, then his/her technique is ineffective. Regrettably, a revision in 3 months, if performed with the same technique, would be unlikely to yield better results.
Because there's no visible swelling (which would plump and smooth the area of wrinkling) my instinct is that the surgeon did not touch the wrinkled areas, nor did s/he elevate the muscle layer so that the skin would be supported in a lifted position. This is essential to improve marionette lines and downturned corners of the mouth.
I believe you could have an excellent result. However, if you're looking for a major result within a minor procedure, you will undoubtedly be disappointed. I recommend a revision total face and neck lift with SMAS tightening and facial fat grafting. Surgery lasts about 5 hours and social down time is 3 weeks. It should be performed by a highly trained, board certified (in plastic surgery), and experienced face lift surgeon. S/he should show you before/after photos of face lift results that impress you. And, to avoid repeat disappointment, clear communication is vital so that you and your surgeon have the same expectations for the outcome.
All the best in your search!- Dr. Rad
Learn more: http://sherberandrad.com/pages/face-aesthetics-face-lift-overview
You might also like...
Two Weeks Postop
Learn more: http://drprendiville.com/services/surgical/face/
Face lift - really disappointed.
- I am so sorry you are disappointed with your face lift -
- Please don't feel stupid - we are all entitled to make mistakes, if in fact you made one.
- At two weeks, you are swollen and things may actually improve a lot.
- But I can see why you are concerned -
- Wait another month and if things are not looking better, at least get a 2nd opinion to discuss why things didn't work out and if your first surgeon's plan seems a good one.
- Always see a Board Certified Plastic Surgeon. Best wishes - Elizabeth Morgan MD PHD FACS
More time
Facelift results
Learn more: http://www.plasticsurgerynola.com/face/lift-new-orleans
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text:
Welcome to
Port Arthur Fire Department
The fire department is committed to providing services for the community which instill a 'safety first' attitude. In addition to responding to emergency situations, we offer educational opportunities in fire prevention, and training for an individual's appropriate reaction to an encounter with a hazard endangering life or property. This includes the use of smoke alarms for early warning of fire; information on portable fire extinguishers {hazard identification, the proper type extinguishers for the hazard, and proper use}; exit drills in the home and stop, drop and roll should your clothes catch fire.
LIFE SAFETY should be the responsibility and focus of everyone!!!
We protect lives and property by delivering an all-hazard prevention and mitigation service which includes: Fire Prevention and Education, Firefighting, Medical Emergency Patient Care, Technical Rescue, Dive and Water Rescue Operations, Hazardous Materials Incident Management, and other services to address threats to our community.


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2-night packages for March 31-April 1, 2017 are ON SALE NOW by calling The Broadmoor at 844-476-6873, or CLICK HERE to reserve your package online!
2-night Packages include:
•Classic Accommodations for Friday and Saturday nights
•Tickets to Friday and Saturday night performances, including exclusive after-hours shows
•Admission to VIP Artist Experience
•Exclusive sign-up for experiences and activities
•25% discount on Suite upgrades
•20% discount on golf greens fees
•Complimentary treatment enhancement with purchase of $100 or greater spa service …and more
Two-Night Package prices start at $297.50 per person per night, based on double occupancy. Limited availability. Not applicable to groups. Rate does not include taxes, service fees or parking.
This event sells out every year and has limited availability so don't miss this opportunity. To place your reservation for the three-night weekend package, please call The Broadmoor at 844-476-6873 or visit www.broadmoor.com.
CONCERT ONLY TICKETS: 2-Day Concert-only tickets for Friday 3/31 and Saturday 4/1 are now available at WeekendofJazz.com/Tickets. Tickets are also available for Friday 3/31. Details are available at Weekendfjazz.com/Tickets.
*Lineup & Performers subject to change without notice. Limited availability.


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Playing the video of torturing Lawyer Gao Zhisheng just as it happened
The website of the US-based biweekly "Human Rights In China" reported yesterday that according to the news from a reporter of Xinhua News Agency who uses an alias, the case on Ai Weiwei is over for the time being. Beijing Public Security Bureau used torture to obtain a confession and showed Ai Weiwei the video of torturing Lawyer Gao Zhisheng years ago. Fu Zhenghua, Director of the Public Security Bureau, claimed that "whatever we used in dealing with Gao Zhisheng, we'll use that to subdue Ai Weiwei." After continuous torture, Ai Weiwei was forced to admit tax evasion. Ai's family called on the outside world yesterday to show concern on this incident.
Ai Weiwei was taken away on April 3 at an airport when he was trying to leave China. 20 days has passed and there has been no news of him. The website of the biweekly Human Rights in China posted a report yesterday by the reporter of the Xinhua News Agency who uses an alias as Rong Shoujing that on April 19, officials from the headquarters of Xinhua News Agency in Beijing and the Department of Propaganda of Ministry of Public Security confirmed that the case involving Ai Weiwei's tax evasion is over. The report also quoted an official with conscience at the Ministry of Public Security Bureau that Ai Weiwei suffered torture during the interrogation as the police wanted to get a confession from him.
Getting himself into trouble by leading artists in a demonstration
Officials pointed out that Ai Weiwei's case is jointly handled by the Economic Investigation General Brigade of Beijing Municipal Public Security Bureau and the General Brigade of Domestic Security Protection Squad. Fu Zhenghua, Director of the Municipal Public Security Bureau, told the agents in charge of this case to show Ai Weiwei the video of torturing Gao Zhisheng they took a few years ago, including insertion of a police baton into Gao's anus with blood, semen, urine and feces running down his body. Fu also gave the order: "Whatever we used in dealing with Gao Zhisheng, we'll use that to subdue Ai Weiwei." After a few days of torture, Ai Weiwei was finally forced to sign on a statement of confession and admitted tax evasion.
Sister Gao Ge is concerned
Though there has been no confirmation of this news, the outside world and Ai Weiwei's family are paying great attention to this. During a telephone interview with a foreign media, Ai's sister Gao Ge said she is very concerned that her brother would suffer torture. She again calls on the authorities to tell the public the whereabouts of Ai Weiwei. In the meantime, she hopes that since there is no way to confirm the allegation, she wants to broadcast this to the entire world to make the outside world pay attention to this incident.
Artists protesting forced demolition
Demonstrating in Chang'an Street which infuriated the Beijing officials.
On the early morning of February 22 of last year, Chuangyi Zhengyang Art District located in Changdian Village of Jinzhan Town of Beijing was forcibly torn down. The authorities not only sent over bulldozers, it also dispatched over 100 strong-bodied men dressed in dark-colored clothing who wore masks. They carried with them clubs and broadswords and approached the artists guarding the art district in a frenzied assault with clubs and broadswords, resulting in the injury of seven artists, including the Japanese artist Satoshi Iwama. The incident brought indignation of the artists. At 4 p.m. on the same day, Ai Weiwei led 16 artists in a demonstration in Chang'an Street. The injured artists sat in the wheelchairs and displayed such banners as "Severe Punishment on the Killers! Crack down on Underworld and Evil Gangsters!" "We Want Our Human Dignity Back!" "Citizens' Rights." They walked from East Chang'an Street toward Tiananmen Square. It is the first such protest demonstration after the massacre on June 4.
Ai Weiwei was not only the organizer, he was also in charge of photography and news report. After the demonstrators walked for about 20 minutes, they were blocked by a large number of police and police vehicles. Again, Ai Weiwei, on behalf of the artists, negotiated with the police. The incident attracted the attention of the world media and was breaking news at the time.
Because of this incident, Beijing Municipal Public Security Bureau was reprimanded by its superior. Fu Zhenghua who had just become Director of the Public Security Bureau lost a lot of face and almost lost his job. Besides this, Ai Weiwei has taken an active part in the past few years in other non-government rights defense events, such as claiming damages from the government for parents whose young children have got kidney stones because of the melamine tainted milk. Give these activities, Ai has become the thorn in the flesh in Beijing authorities.
Police mistreating Gao Zhisheng
Electric shocks on anus. Tooth-picks inserted into the private part.
In November 2007, Gao Zhisheng published on the Internet how he was brutally kidnapped and brutally tortured by the police. The means the police adopted against him were ruthless. They beat him in the head and the body and took off all his clothes and gave him electric shocks with electric batons in the anus and private part. They inserted toothpicks into his penis and smoked his nose and eyes with burning cigarettes. It is shocking to read it and it is too brutal to look at.
Gao's article caused a sensation in the outside world. Because of this, he again went missing. In early 2009, his wife, son and daughter fled China and went to reside in America. In April 2010, Gao reappeared in Beijing for some time and received interviews from foreign media. After that, the outside world again lost contact with him. There is a report that Gao Zhisheng is now in Xinjiang under supervised residence. The US and other Western countries have demanded on many occasions that China release Gao Zhisheng.
China and the US will hold a human rights dialog next Wednesday on April 27 in Beijing. The US State Department issued a statement two nights ago, saying that the US will raise the issue of disappearance of Ai Weiwei and other issues such as forced disappearance of many Chinese dissidents and freedom of religion.
Congressman's statement in support of Ai Weiwei.
The human rights dialog between the US and China started in 1990. In 2004, because the US supported a UN bill criticizing China in human rights issues, China announced termination of the dialog and it was not resumed until last year. After the dialog between the US and China on human rights, some events and incidents have happened, such as the winning of Nobel Peace Prize by Liu Xiaobo and the "forced disappearance" of the artist Ai Weiwei.
The arrest of Ai Weiwei has brought concerns from the US mainstream society and media. Quite a few Congressmen in the US, including the senior senator Joe Lieberman who has made a statement in support of Ai Weiwei. The person in charge of the US side in this upcoming dialog is Michael Posner, Assistant Secretary of State in the State Department for the Bureau of Democracy, Human Rights and Labor.
A family letter from his adolescent years is published: "I want to go my own ways."
The family letter was written in 1978. Ai Weiwei was 21 then and was a student at Beijing Film Academy. The family letter described his memories of the childhood years: No beautiful fairy tales, no infinite family feelings, no colorful flowers. There was only an emaciated child carrying firewood in a smoking land, abused and bullied everywhere he went…. "Child at such a young age suffering such evils." It is written in the family letter that he had now a little awakened to the ideal that "One must be one's own master in life and must have a purpose in life and must go his own ways." "Whatever one does, one must do it whole-heartedly. One must have determinations and think further into the future and do not depend on smartness only." Finally, he said: "I have confidence and I will work hard and live up to my brother's expectations."


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Got SEPM 12.1 running on a VM XPSP3(what I had a spare license of), all my clients are communicating back status info but they do not appear to be grabbing the latest definitions.
SEPM has 8/18 defs atm but all clients are still using their install date defs except for one which I manually ran live update on. Checking the client status logs (on the machine) specifically Client Management -> System log. I see entries that state "SEPM is available to provide updates, so scheduled Live Update was skipped.
Not sure if I have a misconfiguration on the SEPM or am missing some firewall exceptions using Windows firewall not the SEP firewall [have hardware firewall at gateway]. I am using the install default policy for liveupdate routines. Searching the help file for "port" yielded no results and web searches largely result in configuring the SEP firewall.
Default settings for the LiveUpdate: Fetch from management server / liveupdate server. Proxy to use system settings, LU scheduling every 4hr w/ 2hr retry. Idle delay on, optional skipping LU older then 2days/8hr. Allow manual LU, allow user modify proxy settings for LU
Any help would be appreciated.
Aug 19, 2011 at 1:53 UTC
Brand Representative for Symantec
Few things to check here.
1) Why is the SEPM carrying older definitions??
SEPM is suppose to carry the Latest definitions, atleast when you have the clients as managed.
Could you run the Liveupdate from the SEPM.
For the same you may have to go to Admin > Server > Highlight Local Site> Click on Download Liveupdate Content
2) Check the Exceptions in the Firewall.
Here is the Article:
3) Check the SEPM --- > Client Communication, here is the Article:
4) Did you also, upgrade the Disk space before migrating to SEP 12.1
Hope this may help you!!!
11 Replies
Aug 19, 2011 at 1:53 UTC
Brand Representative for Symantec
Few things to check here.
1) Why is the SEPM carrying older definitions??
SEPM is suppose to carry the Latest definitions, atleast when you have the clients as managed.
Could you run the Liveupdate from the SEPM.
For the same you may have to go to Admin > Server > Highlight Local Site> Click on Download Liveupdate Content
2) Check the Exceptions in the Firewall.
Here is the Article:
3) Check the SEPM --- > Client Communication, here is the Article:
4) Did you also, upgrade the Disk space before migrating to SEP 12.1
Hope this may help you!!!
Aug 19, 2011 at 4:04 UTC
1) SEPM is holding the latest definitions (now 8/19/11 r7 as of this posting)
Though manually running per your steps gives a "encountered one or more errors. Return code = 4"
2/3) those are exactly the documents I was wanting to have. I suspect its an un-opened port or process.
4) Fresh install, I didn't configure any restrictions so it should have access to 10+ GB of space
*Investigation suspension till Monday :)
Aug 26, 2011 at 12:46 UTC
First off, I was reading the panel wrong and SEP was not recieving updates from online (error code 4).
uninstall / reinstall live update per a forum suggestion enabled it to process correctly. Though I was still getting install errors.
I am thinking that since my VM was set on thin provisioning was causing an issue as live update kept saying it didn't have enough space (despite datastore being set to 2gb [max]). Either way did a restart on that VM and windows was bluescreening.
Next day, finished reinstalling the VM and beginning SEPM install. Don't really think its symantec's fault on this one.
Side question, VM assigned the same MAC address, I used the same comp name. When I reinstall SEPM will the clients auto-add themselves to the system? If not that fine, just preparing myself for 'what-ifs'
Aug 26, 2011 at 1:05 UTC
Brand Representative for Symantec
We always recommend you that you are prepared with the Disaster Recovery steps (just incase)
It is always recommended you to keep the same IP and host name to the SEPM server. This will ensure the Clients report back to the same SEPM.
Incase, if the clients are not reporting to the SEPM, you always have a choice, you can get those clients back by replacing the Sylink.xml to all the clients.
There is a Utility which may assist you to remotely change the Sylink.xml - SylinkReplacer.
Check this Article:
Hope this may help you!!!
Aug 27, 2011 at 3:45 UTC
Well it appears to only have created the Mac install packages this install (none on my network). Did manage to find the files eventually in the SEPM install folder. No idea why it only installed 1 of the 3 despite them being right next to each other.
Does symantec provide a VM image for SEPM to just load and go?
I plan to take an image of the system once I get everything running correctly.
Aug 27, 2011 at 4:31 UTC
Hmm trying to export a package for a group install.
Export Package : Error dialog box
Not able to export package Sep.msi for group Workstation at this time, please retry it later.
I fail to see how retrying it later will be any different.
Aug 29, 2011 at 6:31 UTC
Brand Representative for Symantec
You may like to watch this 2 Threads which may assist you to resolve the issue:
Hope this helps!!
Aug 30, 2011 at 12:29 UTC
Ok, seems like the magic thing was "uninstall, and reinstall"
Live update wasn't processing on SEPM system : reinstall resolved
SEPM was not making packages to deploy onto system : reinstall resolved
Pushed package to client was not connecting to new SEPM server : uninstall client, and redeploy from SEPM
So one last quick question: Is there an easy way to uninstall SEP from all systems (not connected to SEPM) so that I can push out the new client package with info for the new install? In "Install client settings" I have "Remove all previous logs and policies, and reset the client-server communications settings" but yet, a re-deploy is not linking to the new SEPM unless I manually uninstall SEP from the client PC first.
Aug 30, 2011 at 1:48 UTC
Brand Representative for Symantec
If these clients are not reporting to the SEPM, there is no easier way to uninstall SEP clients.
In your case, this could happen when you have Tamper protection Turned on.
I would say, Turn off the Tamper protection and then Manually replace the Sylink.xml to those clients which are not reporting to the SEPM.
Here are the Steps:
1) Login to the SEPM
2) Select the group, right click export communication settings put this file on a shared drive or usb as it needs to be replaced
3) Disable the Tamper Protection protection from SEPM by: Going in Policies > General Setttings> Tamper Protection > Uncheck Tamper Protection
4) Go to client computer
5) Go to start run type smc -stop
6) Go to C:\Documents and Settings\All Users\Application Data\Symantec\Symantec Endpoint Protection\12.1.671.4971.105\Data\Config
7) Replace file exported above in here simply paste it
8) Go to start run and type smc -start
9) Check if the client comes on line
10) Once online, Enable the Tamper Protection from SEPM.
Once, the client communicate to the SEPM, simple assign packages to the Groups for AutoUpgrade.
Hope this helps!!!
Aug 30, 2011 at 3:58 UTC
Hmm, while that could work. If i'm having to go to every machine anyway I might as well just uninstall then issue another remote push.
Thanks though, you have been very helpfull!
Aug 31, 2011 at 9:23 UTC
For reference
A howto on using the WMIC to remote uninstall applications.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
Archinect - News 2017-07-27T12:51:43-04:00 http://archinect.com/news/article/149954862/to-pour-concrete-in-mid-summer-phoenix-wait-until-the-moon-comes-out To pour concrete in mid-summer Phoenix, wait until the moon comes out Alexander Walter 2016-06-29T12:59:00-04:00 >2016-07-02T23:03:19-04:00
A team of construction workers is pouring concrete onto the frame of a structure that will eventually become a wastewater treatment plant. It's 1 a.m. on a clear night in the suburbs of Phoenix. The temperature is still in the high 80s. But that's way down from the area's recent record high temperatures, up to 118 degrees. [...] "We try to pour and place and finish concrete when it's below 90 degrees," says Daniel Ward, the construction company's project director.

Related stories in the Archinect news:

L.A.'s urban heat island effect accounts for temperatures up to 19 degrees hotter

Can Phoenix un-suburbanize?

"7,000 construction workers will die in Qatar before a ball is kicked in the 2022 World Cup," new ITUC report finds

 http://archinect.com/news/article/149950867/this-atlas-shows-how-much-light-pollution-has-infiltrated-night-skies-worldwide This atlas shows how much light pollution has infiltrated night skies worldwide Justine Testado 2016-06-10T21:08:00-04:00 >2016-06-17T21:07:25-04:00
Where should you travel if you want killer views of the stars unblemished by artificial light?Certainly not the U.S. or Europe, where nearly 100 percent of the population endures some form of light pollution...Italy's [ISTIL], NOAA, the National Park Service, and elsewhere built one of the most comprehensive atlases of global light pollution to date. They hope their work will set a benchmark for future generations struggling with day blending into night.

You can find the atlas of artifical sky brightness here.

More on Archinect:

New glow-in-the-dark cement could illuminate roads & structures

Designing for the Night

"drawing/space" by Emma McNally to show at "Abstract Drawing" exhibition in London's Drawing Room
 http://archinect.com/news/article/122818954/designing-for-the-night Designing for the Night Nicholas Korody 2015-03-13T15:10:00-04:00 >2015-03-15T18:41:19-04:00
When I speak with a student about nightlife they have something different in mind than a 65-year old town planning manager. In the municipalities, finding contacts is difficult - often nobody feels responsible or capable of speaking. That should change.

There are sleepy cities and cities that never sleep. There are cities famed for their raucous nightlife, and others whose adolescent residents dream of leaving. According to the German urban scientist Jakob F. Schmid, interviewed for DW.DE, "Nightlife often defines the character of entire streets or districts." Schmid runs the "City After Eight - Management of the Urban Night Economy" project with Thomas Krüger, which is funded by the German government.

Looking at a variety of German cities including Berlin, Hamburg, and Frankfurt, the researchers mapped nightlife "with online recommendation platforms as the basis for the data." The hazy blue results indicate that various forms a city's nightlife may take. In the famed-party city Berlin, for example, hotspots are diffused throughout the expansive metropolis. In a traditional German city, on the other hand, the nightlife tends to be concentrated "on the city center, usually on the streets directly surrounding the center proper."

In the...
 http://archinect.com/news/article/78926701/the-nocturnal-face-of-architecture-awp-s-vers-un-climat-exhibit-opens-this-month The Nocturnal Face of Architecture: AWP's "Vers un climat" exhibit opens this month Justine Testado 2013-08-07T19:37:00-04:00 >2013-08-07T19:42:42-04:00
 Cities can transform into an entirely new place once it's nighttime, sometimes to the point of disorientation that you wonder if you're still in the same city. The upcoming "Vers un climat: Building (With) The Unstable" exhibit at Cornell University presented by award-winning interdisciplinary practice AWP explores the alluring atmosphere of the urban nightscape to illustrate how structures can evolve, change, and perform as active participants in our cities.

 "Vers un climat" is based on years of in-depth research by AWP founders Marc Armengaud, Matthias Armengaud, and Alessandra Cianchetta, who continuously explore how intangible elements like climate, time, and light affect architectural design.

 The exhibit will showcase AWP's realized and proposed designs that convey the practice's recurring themes of impermanence, evolution, and the uncontrollable. Featured projects include The Lantern Pavilion in Norway, the Troll installation pieces, the LAM Museum sculpture park, a...
 http://archinect.com/news/article/46264091/la-light LA Light Alexander Walter 2012-04-25T16:58:00-04:00 >2012-04-30T09:11:14-04:00
 Stunning time-lapse video of Los Angeles at night that recently made it on the 2012 Vimeo Awards shortlist in the category 'Lyrical'.

 Here some more info from Colin Rich, maker of LA Light:

 I sought out to capture the electric radiance of Los Angeles at night and paint a portrait of my city.

 It took me 6 months of on and off shooting to finish this project.

 Shooting time lapses is a labor of love and a study in patience.

 This video is dedicated to the memory of my Grandmother. She spent most of her life bettering the lives of others and was exemplary of what humanity can be in its purest form.

 Cinematography, direction, and editing by Colin Rich
 Production Company: Pacific Star
 Produced by William Ahmanson


text:
Walkthrough part 7
EditBanjo-Kazooie: Nuts & Bolts Walkthrough
|Showdown Town
|Nutty Acres
|Logbox 720
|Banjo Land
|Act 1
|Act 2
|Act 3
|Act 4
|Act 5
|Act 6
|Nutty Acres Act 6
|Jiggies Required // 47
|Jiggies // 5
|Jingo Tokens // 1
|Riverbed Head-to-Head
|Who // Klungo
|Trophy Time // 2:00
It can get feisty really quickly since the race course falls on a dried-up, narrow riverbed. Get a set of fast but narrow wheels—you will probably need to build one. Tack on two of the best engines you've got (should be a large by now) and hit the race course! Blow your opponents away with your ultra-tricked out race wheels.
|Freewheel Festival
|Who // Trophy Thomas
|Trophy Time // 1,500
Another speed trial, though without the help of an engine. Relying strictly on the forces of inertia, you need to pilot your car on the downhill of the volcano, which provides that initial momentum, but once the terrain flattens out you need to quickly find more downhills or you'll stop completely from the friction. Make sure to not hit anything as well, as that definitely will cut out a considerable amount of the built-up momentum.
|Nut to Go
|Who // Humba Wumba
|Trophy Time // 1:10
As you lug the lovely bunch of coconuts around, you will be assaulted by Grunty's ever annoying Gruntbots who are keen on sabotaging this shipment. Stick to flat roads and oversee the successful transfer of the crops to the shipment docks.
|Air Coconut
|Who // Bottles
|Trophy Time // 3:00
This race is not easy, and unlike most of the races that have been mainly grounded, this one puts you in the sky along with several other vicious pilots. Expect your competitors to be fast, so if you can't beat them in speed you can at least gun them down and be that thorn in their side by installing an Egg Gun or two on the plane. During the race, aim and shoot with the intent of blasting your opponent's engines. That way, you slowly gain the lead on them as they spiral out of control or lose speed from blown-out engines. Note that you can't rely entirely on the Egg Guns; you still need a couple of engines and at least one mounted Small Jet.
|Makin' Waves
|Who // Mr. Fit
|Trophy Time // 1:30
Another boat race. As usual, we highly recommend mounting an Egg Gun to the floating vehicle on top of the usual engines. Stick on a Large Propeller too for added mobility and you've got yourself a race.
|Jinjo Hurl
|Who // Orange Jinjo at the top of the volcano
The target distance is set at the 500 mark. Get a mean set of wheels with speed and power to hurl the Jinjo into the air and down the volcano.
|Give us feedback!
|Continue Walkthrough...
Top Wiki Contributors
Need assistance with editing this wiki? Check out these resources:
Open References & Sources (1)
What Links Here
Walkthrough part 2 | Walkthrough part 3 | Walkthrough part 4 | Walkthrough part 5 | Walkthrough part 6


text:
Solulin reduces infarct volume and regulates gene-expression in transient middle cerebral artery occlusion in rats
Thrombolysis after acute ischemic stroke has only proven to be beneficial in a subset of patients. The soluble recombinant analogue of human thrombomodulin, Solulin, was studied in an
Male SD rats were subjected to 2 hrs of transient middle cerebral artery occlusion (tMCAO). Rats treated with Solulin intravenously shortly before reperfusion were compared to rats receiving normal saline i.v. with respect to infarct volumes, neurological deficits and mortality. Gene expression of IL-6, IL-1β, TNF-α, MMP-9, CD11B and GFAP were semiquantitatively analyzed by rtPCR of the penumbra.
24 hrs after reperfusion, rats were neurologically tested, euthanized and infarct volumes determined. Solulin significantly reduced mean total (p = 0.001), cortical (p = 0.002), and basal ganglia (p = 0.036) infarct volumes. Hippocampal infarct volumes (p = 0.191) were not significantly affected. Solulin significantly downregulated the expression of IL-1β (79%; p < 0.001), TNF-α (59%; p = 0.001), IL-6 (47%; p = 0.04), and CD11B (49%; p = 0.001) in the infarcted cortex compared to controls.
Solulin reduced mean total, cortical and basal ganglia infarct volumes and regulated a subset of cytokines and proteases after tMCAO suggesting the potency of this compound for therapeutic interventions.
Stroke is a major cause of morbidity and mortality in the Western civilization. Roughly 60% of ischemic strokes are attributable to large-artery occlusion by thrombembolism. Complete absence of perfusion results in irreversible brain damage and neuronal loss in the stroke core. However, the surrounding penumbra contains functionally impaired, yet reversibly damaged neurons which are potentially salvageable. The goal in modern stroke therapy, therefore, is to protect the penumbra. To date, the only approved drug for lysis therapy is recombinant tissue plasminogen activator (rtPA) which has shown significant benefit in patient outcome when given up to 4.5 hours of onset. Less than 10% of all acute stroke patients are eligible for this treatment. No clinical trial has been able to demonstrate clear beneficial effects in respect to improvement of short- or long-term outcome after anticoagulatory therapy in acute ischemic stroke. Therefore, there is still a need for safe anticoagulatory substances with sufficient antithrombotic effectiveness and minimal risk of hemorrhagic side effects.
Thombomodulin (TM) is an endothelial cell transmembrane protein that acts as a thrombin receptor to modulate coagulation and fibrinolysis
In addition, anti-inflammatory and anti-apoptotic activities mediated by the lectin-like domain of TM are also discussed
In this study we analyzed whether Solulin, besides its known antithrombotic effects, exerts neuroprotective effects under transient ischemic conditions. Primary outcome parameter was infarct volume. Secondary outcome parameters were neurological outcome, mortality, hemorrhagic adverse events, and gene expression. A set of pro-inflammatory cytokines and proteases and microglial (CD11B) as well as astroglial (GFAP) markers were analyzed in the penumbra after 2 hours of tMCAO.
Study drug
Solulin was provided by PAION Deutschland GmbH (Aachen, Germany).
Animals and transient middle cerebral artery occlusion (tMCAO) procedure
Research and animal care procedures were approved by the Review Board for the Care of Animal Subjects of the district government (LANUV (Landesamt für Natur, Umwelt und Verbraucherschutz, Northrhine-Westfalia, Germany)).
Male Sprague Dawley rats (250-280 g, Harlan Laboratories, Boxmeer, Netherlands; n = 10) were randomized to group 1 (n = 5) = control group without treatment or group 2 (n = 5) = treatment with 1 mg/kg body weight (BW) Solulin i.v. in normal saline (injection volume 5 ml/kg BW). Previous preclinical
Animals were held in macrolone cages (Ehret GmbH, Emmendingen, Germany) in a pathogen-free environment with food and water available ad
The left common carotid artery (CCA), internal carotid artery (ICA) and external carotid artery (ECA) were exposed through a midline neck incision. ECA and CCA were ligated proximally and the vagus nerve carefully dissected from the ICA. A 0.1% poly-L-lysine
To assure appropriate MCA occlusion, regional cerebral blood perfusion (rCBF) over the left MCA was performed by laser-Doppler flowmetry (LDF). Through a small midline incision a 2-mm non-invasive laser-Doppler probe (PeriFlux System 5000, Type PF 5001, Perimed, Sweden) was placed on the animal's skull approximately 1-3 mm posterior to the bregma and 2-4 mm lateral to the midline. Baseline measurements were taken 10-15 minutes before tMCAO. Compared to baseline LDF showed a reduction of rCBF by at least 60% after MCAO
TTC-staining and measurement of infarct volume
Prior to brain dissection and mRNA extraction animals were deeply anesthetized and perfused transcardially with normal saline (0.9%) to wash out remaining circulating blood cells within the brain. Rat brains were then removed immediately, cut into 2 mm thick coronal sections using a rat brain matrix (Plastics One Inc., Roanoke, VA, USA) and stained in a 2% solution of 2,3,5-triphenyltetrazolium chloride (TTC) in normal saline (Bederson
V infarct = Volume infarct, V ipsi = Volume ipsilateral hemisphere, V contra = Volume contralateral hemisphere, V edi = Volume edema corrected infarct
Assessment of neurological deficits
Before euthanization 24 hrs after tMCAO, neurological testing was performed with a 6-point scale
5 = normal motor function, no neurological deficit, 4 = flexion of torso and contralateral forelimb when lifted by the tail, 3 = decreased resistance to lateral push without circling, 2 = circling to the contralateral side against resistance when tugged by the tail on a flat surface, 1 = circling spontaneously to the contralateral side, 0 = no spontaneous motor activity, loss of walking or righting reflex.
RNA isolation
On the basis of the TTC-staining results, total RNA was isolated from cerebral cortex of the adjacent penumbra regions of the infarcted hemispheres and the corresponding regions of the contralateral hemispheres from 3 consecutive coronal sections of each brain (sections 2,3 and 4) with NucleoSpin®RNA II (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) according to the manufacturers' instructions.
Briefly, brain tissue was homogenized using Precellys Keramik-Kit (PEQLAB Biotechnologie GMBH, Erlangen, Germany) at 5000 × g for 15 s, cells were lysated with β-mercaptoethanol, filtrated by centrifugation, washed in 70% ethanol, DNA was digested, RNA purified in several washing steps and eluted in RNAse and DNAse free ultra-pure water (Invitrogen GmbH, Darmstadt, Germany).
Gene expression
RNA concentration and purity were assessed using OD260/OD280 ratios (NanoDrop1000, PEQLAB Biotechnologie GmbH, Erlangen, Germany) and gel electrophoresis under denaturation conditions. Reverse transcription reactions were performed using M-MLV RT-kit (Invitrogen GmbH, Darmstadt, Germany) and random hexanucleotide primers. For gene expression analysis, we studied transcription levels of following factors: IL-6 (interleukin-6), IL-1β (interleukin-1β), TNF-α (tumor necrosis factor-α), MMP-9 (matrix metalloproteinase-9), CD11B and GFAP (glial fibrillary acidic protein) (all primers: Invitrogen GmbH, Darmstadt, Germany). All primers were designed with free access software (Primer3, Simgene.com). Primer sequences are listed in table
Table 1
Oligonucleotide primers for RT-rtPCR
Product size
s 5'-GGTCCATTCCTATGACTGTAGATTTT-3'
as 5'-CAATCAAGACGTTCTTTCCAGTT- 3'
125 bp
s 5'-ACAGTGCATCATCGCTGTTC-3'
as 5'-CCGGAGAGGAGACTTCACAG-3'
160 bp
s 5'-CTGTGACTCGTGGGATGATG-3'
as 5'-GGGATTTTGTCGTTGCTTGT-3'
209 bp
s 5'-CTCCCAGAAAAGCAAGCAAC-3'
as 5'-CGAGCAGGAATGAGAAGAGG-3'
209 bp
s 5'-GTCCGGTTTCAGCATGTTTT-3'
as 5'-CCACCGAGCTATCCACTCAT-3'
158 bp
s 5'-TTACCGGACTGTGTGGACAA-3'
as 5'-AGTCTCCCACCACCAAAGTG-3'
239 bp
s 5'-AGAAAACCGCATCACCATT-3'
as 5'-GCACACCTCACATCACATCC-3'
187 bp
Sample size for the primary outcome parameter infarct volume was calculated. From prior experiments mean infarct volumes were expected around 300 mm3 ± 35 mm3 with an anticipated 30% reduction of infarct volumes in the Solulin-treated cohort. The power was set to β = 0.8 and the significance level to α = 0.05 yielding a calculated sample size of four animals per group. In this study five animals per group were included. Power calculation was performed using nQuery Advisor® Version 7.0 (Statistical Solutions, Saugus, Massachusetts, USA).
Edema corrected infarct volumes, and edema corrected mean cortical, basal ganglia and hippocampal infarct volumes were evaluated by two-way ANOVA. Multiple comparisons (heart rate, blood gas measurements and body temperature) were evaluated using an unpaired t-test. Neuroscores and gene expression data were evaluated by non-parametric Mann-Whitney-U-test with SPSS software version 17.0 (SPSS Inc., Chicago, IL, USA). All data are given as arithmetic means ± SEM (standard error of the means), or as means ± SD (standard deviation). P ≤ 0.05 was considered statistically significant. GraphPad PRISM® (GraphPad Software Inc., La Jolla, CA, USA) was used to generate the figures.
Edema corrected Infarct volumes
Edema corrected total infarct volumes were assessed, as well as infarct volumes separated for cortex, basal ganglia and hippocampus.
Brain edema formation in each group was determined indirectly by use of the above mentioned formula
A significant 27.4% reduction in mean total infarct volumes was achieved in Solulin-treated compared to vehicle-treated animals after tMCAO (Figure
Figure 1
Edema corrected mean total infarct volumes after 2 h of tMCAO 24 h post reperfusion
Edema corrected mean total infarct volumes after 2 h of tMCAO 24 h post reperfusion. Edema corrected infarct volumes (mm3) of Solulin-treated (left; n = 5) and vehicle-treated animals (right; n = 5). Significant reduction of infarct volumes in the Solulin-treated group (p = 0.001). Values are given as means ± SD.
Figure 2
Edema corrected mean cortical, basal ganglia and hippocampal infarct volumes after 2 h of tMCAO 24 h post reperfusion
Edema corrected mean cortical, basal ganglia and hippocampal infarct volumes after 2 h of tMCAO 24 h post reperfusion. A significant reduction of cortical (p = 0.002) and basal ganglia (p = 0.036) infarct volumes was achieved. Hippocampal infarct volumes were nominally but not statistically significantly reduced. Values are given as means ± SEM.
No hemorrhagic transformation was observed in either group.
Heart rate and body temperature did not differ between the groups. All blood gas measurements (pH, pO2, pCO2, cNa+ or cK+) before and during ischemia or after reperfusion were comparable between the groups.
Neurological deficit
Neurological deficits in both groups were equally distributed. Four animals had a score of 2 (circling against resistance) and one animal had a score of 1 (spontaneous circling) in each group, respectively.
Quantitative gene expression
HPRT and cyclophilin A served as a housekeeping gene to normalize gene expressions of brain tissue samples
Figure 3
Gene expression of IL-1β, IL-6, TNF-α, CD11B, MMP-9 and GFAP after 2 h of tMCAO 24 h post reperfusion
Gene expression of IL-1β, IL-6, TNF-α, CD11B, MMP-9 and GFAP after 2 h of tMCAO 24 h post reperfusion. Gene expression of IL-1β, IL-6, TNF-α, CD11B, MMP-9 and GFAP of 3 consecutive coronal sections of TTC-stained hemispheres. The contralateral non-infarcted hemispheres of the vehicle-treated group acted as internal controls. Values of these hemispheres were therefore set to 100%. All other values (infarcted hemisphere vehicle-treated group, infarcted and non-infarcted hemisphere of the Solulin cohort) were expressed as percentages of the latter. RT-PCR values were normalized to the housekeeping genes HPRT and cyclophilin A. All mRNA levels are given as means ± SEM.
Table 2
Gene expression of IL-1β, IL-6, TNF-α, CD11B, MMP-9 and GFAP after 2 h of tMCAO and 24 h post-reperfusion
Contralateral [%]
Ipsilateral [%]
Contralateral [%]
Ipsilateral [%]
1165.5 ± 1522.1
46.6 ± 42.8
242.7 ± 443.4
26.1 ± 22.2
411.7 ± 550.6
206.2 ± 278.9
86.1 ± 92.4
83.9 ± 56.9
257.6 ± 131.1
41.9 ± 46.5
201.6 ± 97.1
236.2 ± 56.1
86.6 ± 85.5
119.6 ± 102.8
108.4 ± 69.5
71.7 ± 89.7
87.1 ± 89.7
Gene expression of three consecutive coronal sections of TTC-stained hemispheres. The contralateral non-infarcted hemispheres of the vehicle-treated group acted as internal controls. Values of these hemispheres were therefore set to 100%. All other values (infarcted hemisphere vehicle-treated group, infarcted and non-infarcted hemisphere of the Solulin cohort) were expressed as percentages of the latter. RT-PCR values were normalized to the housekeeping gene HPRT and cyclophilin A. All mRNA levels are given as means ± SD.
A significant downregulation of IL-1β (79%; p < 0.001), TNF-α (59%; p = 0.001), IL-6 (47%; p = 0.04), and CD11B (49%; p = 0.001) was found in Solulin-treated infarcted hemispheres compared to untreated infarcted brain halves. Also MMP-9 (22%; p = 0.34) was nominally reduced, but the difference was not statistically significant. In the non-infarcted Solulin-treated hemispheres compared to untreated non-infarcted brain halves, gene expression was significantly lower for IL-1β (57%; p < 0.004) but not for TNF-α (p = 0.61), IL-6 (p = 0.063), MMP-9 (p = 0.11) and CD11B (p = 0.34).
No significant difference could be detected in GFAP (glial fibrillary acidic protein) expression neither between injured (p = 0.094) nor between non-injured hemispheres (p = 0.19) in both groups.
Solulin administration caused a down-regulation of the expression of inflammatory cytokines in the penumbra. In particular, TNF-α, IL-1β, and IL-6 were regulated. In addition, we observed that the expression of CD11B, a marker for microglia/macrophage activation, was also significantly reduced in Solulin-treated ischemic animals compared to untreated infarcted controls. GFAP, an astroglia marker, and MMP-9 were not regulated by Solulin. Solulin achieved a significant reduction in mean total infarct volumes (27.4%) with effective protection of both cerebral cortex (19%) and basal ganglia (12.6%). The overall reduction of infarct volumes does not suffice to explain the disparate decreases in expression of the various pro-inflammatory cytokines (IL 1β, 79%; TNF-α, 59%; IL-6, 47%).
Cerebral ischemia triggers a pathogenic cascade in which microglia are locally attracted and activated and express growth factors, chemokines, and regulatory inflammatory cytokines as mediators that attract mononuclear cells and granulocytes which further damage the ischemic brain tissue and its penumbra
TNF-α serves as a marker for activation of microglia and macrophages during cerebral ischemia and other inflammatory reactions as reported previously
There was no difference in expression of glial fibrillary acidic protein (GFAP), neither in infarcted nor in the unaffected hemispheres of both Solulin and control rats. GFAP is the principal intermediate filament in mature astrocytes and serves as a marker for reactive astrogliosis
Matrix metalloproteinases (MMP) are proteases related to extracellular matrix breakdown
As cerebral reperfusion in the current study was produced by removal of the occluding thread, it appears unlikely that anticoagulation is central to Solulin's ability to reduce infarct volumes. Rather, its anti-inflammatory and anti-apoptotic activities, mediated by its lectin-like domain
Nonetheless, there may also be a role for anticoagulation, as restoring blood flow may give rise to downstream displacement of material formed around the occluding suture. Solulin might also act to restore blood flow in the dependent microvasculature, which is likely to get clogged to some extent during the ischemia period and will not readily re-open once the occluding thread has been removed. This possibility is supported by the observation that Solulin, in the mouse photothrombotic model, was able to restore blood flow when administered 30 or 60 min post-occlusion
In response to the treatment with the recombinant soluble analogue of the human transmembrane protein thrombomodulin, Solulin, we could clearly demonstrate a significant reduction in mean total infarct volumes as well as of cortical and basal ganglia infarct volumes in a model of transient focal cerebral ischemia. We could further show that Solulin abolishes the induction of TNF-α, IL-1β, IL-6, CD11B as well as of MMP-9 in the penumbra. The underlying mechanisms of action are not yet fully understood, but seem to involve anti-inflammatory effects, possibly aided by activation of protein C and anticoagulant (profibrinolytic) effects. Requisite to understanding how this promising substance brings about the profound reduction infarct volumes, future neurocognitive studies are indicated.
Competing interests
KUP is an employee of PAION Deutschland GmbH, Aachen. All other authors declare that they have no competing interests.
Authors' contributions
YMR and JD conducted the experimental laboratory work. YMR and MC performed the statistical analysis and drafted the manuscript. KUP and RR participated in the study design and coordination and helped to draft the manuscript. MK, CB, AF and DW helped to perform the study and draft the manuscript. All authors read and approved the final manuscript.
This research was conducted with funding by the Rotationsprogramm of the Medical Faculty of the RWTH Aachen and PAION Deutschland GmbH, Aachen. We thank the team at the Department of Animal Research at the University Hospital Aachen for expert laboratory advice and Mrs. Rosemarie Blaumeiser-Debarry for her outstanding assistance and help.
Regulation of fibrinolysis in plasma by TAFI and protein C is dependent on the concentration of thrombomodulin
The N-terminal domain of thrombomodulin sequesters high-mobility group-B1 protein, a novel anti-inflammatory mechanism
The interaction of thrombomodulin with Ca2+
Elements of the primary structure of thrombomodulin required for efficient thrombin-activable fibrinolysis inhibitor activation
Isolation of a membrane-bound cofactor for thrombin-catalyzed activation of protein C
Complex formation between thrombin and thrombomodulin inhibits both thrombin-catalyzed fibrin formation and factor V activation
Proteolytic inactivation of human factor VIII procoagulant protein by activated human protein C and its analogy with factor V
The mechanism of inactivation of human factor V and human factor Va by activated protein C
Activation of PDGF-CC by tissue plasminogen activator impairs blood-brain barrier integrity during ischemic stroke
Do-all receptor takes on coagulation, inflammation
The lectin-like domain of thrombomodulin confers protection from neutrophil-mediated tissue damage by suppressing adhesion molecule expression via nuclear factor kappaB and mitogen-activated protein kinase pathways
Thrombomodulin-protein C-EPCR system integrated to regulate coagulation and inflammation
Reversible middle cerebral artery occlusion without craniectomy in rats
Middle cerebral artery occlusion in the rat by intraluminal suture. Neurological and pathological evaluation of an improved model
Neuroprotective effects of argon in an in vivo model of transient middle cerebral artery occlusion in rats
Neuroprotective effect of SolCD39, a novel platelet aggregation inhibitor, on transient middle cerebral artery occlusion in rats
Neurological impairment in rats after transient middle cerebral artery occlusion: a comparative study under various treatment paradigms
Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genes
Microglia: neuroprotective and neurotrophic cells in the central nervous system
Tumor necrosis factor-expression in ischemic neurons
Concomitant cortical expression of TNF- and IL-1 mRNAs follows early response gene expression in transient focal ischemia
Expression of tumor necrosis factor alpha after focal cerebral ischaemia in the rat
Inhibition of TNFalpha attenuates infarct volume and ICAM-1 expression in ischemic mouse brain
Inhibition of tumor necrosis factor and amelioration of brain infarction in mice
Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice
Dendritic cells and dendritic-like microglia in focal cortical ischemia of the mouse brain
Cytokine actions in the central nervous system
Expression of interleukin-6, c-fos, and zif268 mRNAs in rat ischemic cortex
Ischemia-induced interleukin-6 as a potential endogenous neuroprotective cytokine against NMDA receptor-mediated excitotoxicity in the brain
Demonstration of increased endothelial-leukocyte adhesion molecule-1 mRNA expression in rat ischemic cortex
Astrocytes: biology and pathology
Extracellular matrix degradation by metalloproteinases and central nervous system diseases
Matrix metalloproteinases and their multiple roles in neurodegenerative diseases
Matrix metalloproteinases and tissue inhibitors of metalloproteinases in cerebrospinal fluid differ in multiple sclerosis and Devic's neuromyelitis optica
Effects of matrix metalloproteinase-9 gene knock-out on the proteolysis of blood-brain barrier and white matter components after cerebral ischemia
Delayed matrix metalloproteinase inhibition reduces intracerebral hemorrhage after embolic stroke in rats
Effects of matrix metalloproteinase-9 gene knock-out on morphological and motor outcomes after traumatic brain injury
Activated protein C
The impact of the inflammatory response on coagulation


text: To get from Partinico a Castrovillari by train you can catch one of the 2 trains that everyday travel between Partinico and Spezzano Albanese Terme, the closest station to the city. The first train to Spezzano Albanese Terme leaves from Partinico at 08:08 and the last train departs at 10:59. If you choose a local train the trip lasts on average 10:08 hours. On Wanderio you can compare all schedules, fares and railway companies to Spezzano Albanese Terme and book the best itinerary starting from Partinico.


text:
EENI- School of International Business
Sub-subject: Denominations of Christianity: Catholics, Protestants, and Orthodox. Syllabus:
Description of the Sub-Subject: Denominations of Christianity.
Like almost all religions, Christianity is not a homogeneous block.
Throughout its 2,000 years of history has experienced three major schisms, forming the three major Christian denominations: Catholics, Protestants, and Orthodox
Roman Catholicism, based in Rome, represents 50% of all Christendom. It is the majority in the Central and Southern Europe, Ireland, and South America.
The Catholic Church has 1,100 million adherents worldwide, representing half of the World's Christian Population. Brazil has the largest number of Catholics in the World (134 million). There are more Catholics in Brazil than in Italy, France, and Spain together.
The ten countries with the largest number of Catholics (Brazil, Mexico, the Philippines, the United States, Italy, Colombia, France, Poland, Spain, and Congo DR) contain more than half (56%, 607 million) of the World's Catholics.
There are sixty-seven countries where Catholics are the majority of the population. Over 70% of Catholics live in the Americas (48%) and Europe (24%). Nearly 40% live in Latin America and the Caribbean. More than a quarter of Catholics lives in the Region of Asia-Pacific (12%) and Sub-Saharan Africa (16%).
The 801 millions of Protestants in the World constitute 37% of the World's Christian Population. The Protestant Reformation, which divided Western Christianity and gave birth to Protestantism, took place in Europe in the sixteenth century. Today, however, only two of the ten countries with the largest Protestant populations are European: The United States, Nigeria, China, Brazil, South Africa, the United Kingdom, the Democratic Republic of the Congo, Germany, Kenya, and India.
There are 260 million Orthodox Christians, who make up 12% of the World's Christian Population. Almost four in ten Orthodox Christians around the world (39%) reside in Russia, the country with the largest number of Orthodox. Ethiopia has the second-largest number of Orthodox.
All other Christian traditions (Mormons, Jehovah's Witnesses) represent 1% of all Christendom.
Number of Christians in the World by continent (Data in million):
Christianity became the official religion of the Roman Empire in 380.
Until 1054, Christianity formed a monolithic block (except small movements as Nestorians). That is, about half of its history, 1000 years, Christianity has been unitary.
Sample of the Sub-subject: Denominations of Christianity
Religions and International Business


text:
The film about me, "Jesseca Ferguson: Cabinet of Curiosity", is now available for viewing in full on Vimeo and on Roberto Mighty (the creator)'s website. Feel free to share it with anyone you think might be interested!
Ex-Libris, Médiathèque André Malraux , Strasbourg, France
April 5 – June 24, 2017
I am one of 26 artists from Boston & Strasbourg who have made artists books in response to the 18th century manuscript of Georg Flohr
Poetics of Light: Pinhole Photography (from the collection of New Mexico History Museum, Santa Fe, NM)
National Media Museum, Bradford, England
March 17 – June 25, 2017
Poetics of Light is the European premiere of an exhibition of original prints of pinhole photography from around the world. The images were collected by Eric Renner and Nancy Spencer in San Lorenzo, USA over the last 30 years, revealing an alternate version of reality and a visual form of poetry.
Organised by New Mexico History Museum, Santa Fe, New Mexico USA
Mirrors: Found in Collection II: Contemporary Photography from the Danforth Art Museum Permanent Collection
Griffin Museum of Photography, Winchester, MA
March 9 – March 31, 2017
Thursday, March 16, 7-9pm: Gallery talk with Jessica Roscio, Danforth Art Museum curator
Found in Collection: Contemporary Photography from the Danforth Art Permanent Collection Part II
March 9 – March 31, 2017
February 20, 2017 (Winchester, MA)__ The second install of Found in Collection: Contemporary Photography from the Danforth Art Museum Permanent Collection will be presented in two parts at the Griffin Museum of Photography. Both paintings and photographs will be exhibited, including work by John Brook, Arno Rafael Minkkinen, Julie Melton, Jesseca Ferguson, Samuel Quinn, David Prifti, Jaclyn Kain, Molly Lamb, Gail Samuelson, and Ruth Thorne-Thomsen, among others. The first install of exhibitions took place at the Griffin Museum of Photography during the month of December 2016. In Part Two Memory will be shown in the Atelier Gallery and Mirrors will be exhibited in the Griffin Gallery as part of Found in Collection: Contemporary Photography from the Danforth Art Museum Permanent Collection from March 9th through March 31st, 2017. An opening reception will take place on March 9, 2017 from 6:30-8:30pm. The will be a curator's talk, with Roscio on March 16, 2017 at 7 PM at the Griffin Museum.
"Memory and absence wind their way through the second part of Danforth Art's two-part exhibition Found in Collection," writes Jessica Roscio, curator for the Danforth Art Museum. "Imagined travel narratives, lost places, and remembered spaces are envisioned in photographs, paintings, and drawings from the late nineteenth century to today…… [and] is apparent in works throughout this exhibition," she says. Roscio goes on to say that the works in Mirror "comment on the surreal aspects of one's interior life, and its collision with an often fantastical and disturbing reality. In turbulent, uncertain times, the allure of an alternate reality, or simply the belief in illusion, appeals to our need for escapism."
Trailer for the new documentary about renowned artist and pinhole photographer – "Jesseca Ferguson: Cabinet of Curiosity". A film by Roberto Mighty. The full-length (12 minutes) film is now available for festival, institutional, broadcast and private screenings. For more information: robertomighty.com. Sneak Preview during Fort Point Channel Open Studios October 15-16.
Ex Libris ExChange
an international collaborative art project
From Oct 7, 2016 To Nov 1, 2016
At The French Cultural Center Get Directions
Opening Reception: Thursday, October 6, 6:30 PM – 8:30 PM
Free and Open to the Public.
"Mirror with a Memory" Alternative Process Photography in the 21st Century.
Curated by Brett Henrikson
Exhibit: October 20 – November 12, 2016
Opening reception: October 20, 2016 from 5 to 9pm (Gallery Night Providence)


text:
Wenn Sie Ihre Suche Verfügbarkeit ändern möchten, benutzen Sie bitte die folgenden Felder:
The Standard Studio of Hotel Afroessa is built according to the Cycladic architecture. It is a one-space room ,dressed with romantic atmosphere. Each studio has a private furnished balcony view to the sunset and the magnificent Caldera.LESEN SIE MEHR
The Superior Suite for Two of Hotel Afroessa is equipped with traditional, wooden furniture, which in combination to the warm lighting and the bright colourings, create a unique surrounding. Here you will feel at home, since everything are chosen very carefully. The Superior Suite has a terrace with magnificent view to the Caldera Gulf.LESEN SIE MEHR
This Studio of Hotel Afroessa is situated at the pool area. It is a one-space room with the same facilities as the Standard Studio with balcony. While the studio does not have its own balcony, it still offers a view to the sunset and the magical Caldera.LESEN SIE MEHR
The Deluxe Studio of Hotel Afroessa is built according to the traditional architecture and blends with the landscape of Santorini. The bedroom with the cast-iron bed or built beds is separated from the sitting area creating the perfect place for relaxation. There is a furnished terrace/balcony with magnificent view to the Caldera.LESEN SIE MEHR
Built on the cliff of the Caldera in Imerovigli, Afroessa is a family run hotel boasting uninterrupted views of the Volcano.
All of the studio & suites open in furnished private balconies offering views to the Aegean Sea and the famous Santorinian Sunset. The interiors are elegantly appointed offering all standard amenities for a comfortable stay.
A traditional canava built in 1700 was renovated in 2013 with respect to the traditional architecture and was transformed to a bar and sitting area. Soak the sun at the sundecks and parasols by the swimming pool and enjoy your drink or snack from hotel's pool bar.
Hotel's staff with its exceptional personalised service will be glad to suggest things to do and see in Santorini, arrange car or motorbike rental, excursions and last but not least make sure you'll have an unforgetable stay.
We welcome you in an idyllic environment ideal for adults who want to live unforgettable moments in the most erotic island. That is why our hotel accommodates only adults and offers luxurious accommodation conditions, thus contributing to the creation of the sweetest memories of your holidays to magical Santorini.
Your credit card will be charged with the 30% of the total reservation cost upon confirmation.
A credit card authorization to the hotel is required in order to confirm a reservation. Your credit card will be charged with the 30% of the total reservation cost upon confirmation. In case of cancellation, this amount will be refunded to you.
If cancellation occurs:
21 days or more prior to scheduled arrival date, no fee is charged
15 to 20 days, prior to the scheduled arrival date, 50% of the total amount of the stay is charged
7 to 14 days prior to the scheduled arrival date, 75% of the total amount of the stay is charged
1 to 6 days prior to the scheduled arrival date, the total amount of the stay is charged
This is an adult only hotel.
Guest orders from menu and pays at check out
Please let us know your arrival details once you know it.We need that information any way.
Due to limited taxis on the island (39), it can be difficult finding one at the airport or port upon arrival.
AFROESSA HOTEL can arrange your transportation from the airport/port as follows:
1: Standard Transfer via minibus at the rate of 27€ for two passengers, one way (May or may not make additional stops on the way for additional passengers)
2: Private Transfer via minibus at the rate of 45€ for two passengers, each way (Direct shuttle with no additional stops. May or may not include other passengers of Afroessa Hotel).
If you are planning on renting a car during your stay, we can arrange a rental and have them meet you at the port or airport.
|lindaFri 21 Jul, 2017
A marvellous, hotel with personal service
This wonderful hotel has friendly personal staff who look after their guests to the highest standards. I would recommend this hotel to all people but would suggest it is not suitable for couples with younger children.
|MeredithFri 21 Jul, 2017
Best Accommodation for Honeymoon
I would HIGHLY recommend this hotel to anyone who is visiting the island of Santorini. The location and view are like no other. You can share photos and write words about how wonderful it is - BUT you must go and experience it yourself! From the manager Mary to Edy, the staff was spot on. They did an excellent job making us feel relaxed and soaking in every minute we had there. I almost felt sad leaving them as much as the hotel. It truly is a special place with a small amount of rooms, which allows all guests to take advantage of the serenity of this special place. I know we were celebrating our recent nuptials, but I think anyone can stay here on a holiday. You will not be disappointed! I wish more hotels extended the genuine customer service that the staff at Afroessa did.
|ThierryThu 20 Jul, 2017
Nice quiet little hotel
Nicely located, at the end of Imerovigli, with a wonderful view over the caldeira. Very nice staff. Be prepared to climb steps as the rooms are on 6 different floors!
|PAARASWed 19 Jul, 2017
Amazing Hotel with Stunning Sunset View
My boyfriend and I loved our stay at Afroessa, we spent 3 nights here and everything from the word go was fantastic. Joanna and Mary were both so helpful when we were booking our stay and this continued once we arrived in Santorini, they were both very warm and welcoming and helped us to book ferry tickets, ATV and taxi hire, they even upgraded our room! The hotel itself is very picturesque, built into the cliff side with views along the coast to Oia and out across the sea and Caldera which took our breaths away. Sunset from our terrace was simply out of this world! The rooms were well maintained and spacious and with there only being a total of 12 rooms in the hotel the pool area was never overcrowded. I would definitely recommend a stay at Afroessa to anyone visiting Sanrotini and hope to come back here in the future!
|AndreasFri 14 Jul, 2017
Very nice small hotel with amazimg caldera view
Polite and helpfool staff, clean rooms, perfect location. Good value for money.
|anneThu 13 Jul, 2017
Loved this hotel
Great hotel. I would definitely come back!
|JenniferThu 13 Jul, 2017
Perfect Hotel
Affroessa is an incredible hotel. The view is amazing from every location. The staff is very attentive and friendly. The room was lovely and comfortable. When I return to Santorini I will definitely stay there again.
|NicholasTue 11 Jul, 2017
Great place to stay in Santorini.
In a quiet area with an excellent crater view yet close enough to walk to Fira. Nice rooms, excellent food and super helpful staff. What more could you ask for!
|DebraTue 11 Jul, 2017
Perfect hotel
Our stay at afroessa could not have been better. It exceeds all expectations. A big thank you to all the wonderful staff who made our stay amazing. Would love to return one day.
|TracyTue 11 Jul, 2017
Tranquil property
|Marie-EveMon 10 Jul, 2017
Wonderful hotel with a spectaculaire
|WendyFri 30 Jun, 2017
Amazing owner Staff and facilities
The owner and long term staff could not have been more helpful. We had an amazing time, with a 40 minute very scenic walk into Fira each day and a short bus ride back, as well as a leisurely lay around the beautiful pool sipping on the occasional cocktail from Eddy at the poolside bar. We would definitely come back without hesitation.
|NoelWed 28 Jun, 2017
Fantastic boutique hotel
I felt like I was staying in a large beautiful Greek home which also happens to offer hotel services. I dont know where to start. From the breathtaking views, to the sumptuous meals (freshly baked croissants every day) to the wonderful staff. Then there is the hotel manager (Joanna) who left no stone unturned to make sure we were comfortable and had a good time. The beautiful architectural features and unique interior decorations that make Afroessa so special will simply amaze you. Suffice it to say that I am already planning my return to Afroessa.
|KathTue 27 Jun, 2017
What an amazing stay we had in beautiful Santorini. The location of the hotel was excellent we were out of the hustle and bustle of Fira (beautiful and easy stroll into town) with a sensational view of the sunsets from our balcony. The staff were fantastic and very attentive.......we could not fault them. We will definitely recommend this hotel to family and friends and intend to return one day.
|RoseanneMon 26 Jun, 2017
Love this amazing hotel
Truly wonderful!


text: Definition Edit A critical update is " a broadly released fix for a specific problem that addresses a critical, non-security-related bug.[1] " References Edit ↑ Microsoft, Description of the standard terminology that is used to describe Microsoft software updates.[1] Retrieved from "http://itlaw.wikia.com/wiki/Critical_update?oldid=93221" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Definition Software Add category Cancel Save