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Lean Manufacturing
Lean ManufacturingA quick definition of Lean Manufacturing, a derivative of the Toyota Production System (TPS), is that it is a set of principles and tools that helps to eliminate process activities that don't add value and to create "flow" in a process.
A Lean process is defined as one that uses only the absolute minimum of resources to add value to the service or product. In a Lean process, no people, equipment, or space are dedicated to rework. Lead times are minimal and everybody involved in the process performs only value-added tasks.
Processes in Lean methods are thought of as value streams. Lead time reduction and the flow of the value streams are the major areas of focus. Value-stream mapping helps teams understand the flow of material and information as the product is being processed through the organization, creating a vision of the entire system.
Lean methods, then, should be thought of as:
- A way to understand "value" from the viewpoint of the customer and eliminate activities that don't add value
- A methodology to create a continuous flow of value-adding activities, pulled by the customer
- A set of tools to continuously improve this flow.
Lean principles and tactics include Pull instead of Push, Continuous Flow, Eliminating Waste, Takt Time, Quick Changeovers, Mistakeproofing (poka-yoke) and Standardization, Visual Management, Value-Stream Mapping, and Kaizen (intensive events where small teams facilitate implementation of Lean principles). They can be applied to manufacturing, service and transactional environments.
Combining Six Sigma and Lean methods can be very powerful if one uses a process management framework to identify opportunities for projects and if management understands how Six Sigma and Lean work together.
Rath & Strong - First to Bring Lean Manufacturing from Japan and First to Apply Lean to Service and Transactional environmentsRath & Strong is among one of the first firms that brought Lean Manufacturing from Japan, and we pioneered in combining process and operations improvement with organizational effectiveness and leadership development.
Rath & Strong and the Roots of Lean Manufacturing
By the early 1980s, Rath & Strong had studied the Toyota Production System (TPS) and recognized in Just-in-Time (JIT) a true multidisciplinary approach to manufacturing. The Just-in-Time Breakthrough, written by Rath & Strong consultant Ed Hay, was a milestone in bringing Japanese-style manufacturing techniques to U.S. companies. Rath & Strong also pioneered Manufacturing Resource Planning (MRP II), applying it and precursors of Lean to processes outside of manufacturing very early. Extending breakthrough work by Rath & Strong colleagues in MRP II, Rath & Strong consultants introduced Value Added Flow Analysis and redefined Value Add in ways that advanced the original Japanese insights. As a leader in areas such as Total Quality Management (TQM) and Just-in-Time, we were at the forefront of bringing innovative process improvement methodologies to our clients.
Learn more about Rath & Strong's Lean Offerings.
Lean Methods Training
Training Courses
Product Sheet
View the Lean Product Sheet
Lean Tools
Related Services
Supply Chain Management Managing a supply chain requires a pragmatic approach. Trying to shape the various elements can become a never-ending struggle – like squeezing a round balloon into a square container. more
Process Redesign At Rath & Strong, we believe process and operations improvement are linked to a company's politics and culture. That's why we address issues of culture and customer focus simultaneously. more


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The Autism News | English By: JO CIAVAGLIA | Bucks County Courier Times Police in four Bucks towns soon will be trained in a jail diversion program designed to prevent violent interactions between cops and people with mental illness. The strange voices that Julie hears four hours a day are sometimes loud and overlap making it hard to focus when other people, like police officers, are speaking to her.
Read more from the original source:
Training police to think differently about mental illness

subset: commoncrawl


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The United States added 10 amendments — a Bill of Rights — to the Constitution in 1791. The second is just 27 words long, and quite easily understood:
Amendment II
A well regulated Militia, being necessary to the security of a free State, the right of the people to keep and bear Arms, shall not be infringed.
The presenter in this video is conservative blogger Bill Whittle. His remarks make more sense than most speeches we've heard recently regarding gun rights:
What would you like to hear leaders say about the Second Amendment and your right to keep and bear arms?


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The Roswell's Belda'r Cafe is an eatery in New Mexico.
The morning after the capture of the Roswell Army Ghosts, Peter Venkman met up with Special Agent Melanie Ortiz at the Belda'r Cafe to warn her about the ensuing paperwork surrounding the case.
- The Belda'r Cafe is a stand-in for the now defunct Cover-Up Cafe. The reveal was the 'Weather Balloon' part cut off by the bottom of the panel.
- The cafe is named after Beldar, a character portrayed by Dan Aykroyd in the Coneheads SNL sketches and movie. "Consume Mass Quantities" is a quote of Beldar.
- The 3-eyed Happy Face is from the Ivan Reitman movie Evolution.
- At the bar is Paul Rudoff of Spook Central.
- Next to Paul with his back turned is Calahan from "Big Trouble With Little Slimer"


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This is a positive post encouraging my friends and people who read this to go check out this blog. I participated, but it's sort of redundant if we don't get any more people. So, for the sake of this wonderful idea, please participate! You get a userbox :D
The second thing I wanted to mention is (once again) in regards to my fanon. In the words of the great Fire Lord Zuko, "I've lost my stuff". I am at a roadblock. Writers block. A sheer cliff threatening to crush me. It's terrible, I may cry, or crash and burn. For those few people who have read it, if you could give me some, a few, any ideas? Any at all?


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Rasheed Wallace
Fatman Scoop TV: Episode 1
This news magazine-style release observing the often interweaving areas of music, fashion and business features radio personality Wendy Williams, who's known for speaking her mind and being rather direct with those to whom she talks. Highlighted are interviews with stars such as Ludacris, Elephant Man, Wyclef Jean, Kanye West and Rasheed Wallace. Revealing, interesting and funny, this collection provides a ton of industry info.


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School Info
Principal: Dr. Jennifer Yacoub
Vice Principal: Wadean Van Ruler
To Report An Absence:
(209) 668-9600
Parent Info
Bell Schedule
We have made some changes to our safety protocols as we want to tighten security and insure safety for all at Medeiros. We appreciate that all parents follow these protocols at all times.
· Parents need to check into and check out of the office at all times when entering the campus to volunteer, accompanying a field trip, having lunch with their child, dropping off lunch and if picking up their child for any reason before school ends.
· Parents and volunteers need to make sure that they have a visitor sticker or a volunteer badge at all times when on campus grounds.
· During dismissal, we ask that anyone picking up children to wait by the flag pole and by the fences by the park or McKenna. We do not want parents to wait by the classroom doors.
· If anyone is roaming around campus, they will be referred to the office to check in and get a visitor badge.
School Visitors
Parents/guardians and other guests of the district are welcome and encouraged to visit TUSD schools. Visitors must respect the learning environment however and maintain proper behavior and decorum. (EC 51512)
Disruption of the orderly process of the school is prohibited. In accordance with Penal Code 626.7, the principal or his/her designee is authorized to deny access, ban or bar future access, and remove or request the removal of any visitor whose behavior is disruptive to the educational/ working environment of the school.
Visitors to any of our campuses who have been denied access or who have been asked to leave may schedule an appointment to appeal to the Director of Student Services at (209) 667-0887 daily between the hours of 8 am and 4:30 pm.
Staff Info
The mission of Sandra Tovar Medeiros Elementary School,
an exemplary and diverse learning community, is to ensure
that all students excel academically and demonstrate
positive character traits in a dynamic, safe, student-centered
environment by delivering creative, rigorous instruction along
with comprehensive curriculum to maximize individual student
potential in collaboration with parents, families, and the community.
Our 1st grade team is 120 years old. We think they are aging quite nicely :)
TK and Kindergarten STEM Challenge
Congratulations to our wonderful campus supervisor JENNY DESOUSA!!!
Kindergarten classes celebrating the 100th day of school with Zero the Hero!
Medeiros 3rd Grade Students Making a Difference
Here is an article in the Turlock Journal about our third grade classes making blankets for an organization called FOCUS. We are very proud of our third graders for helping others.
Upcoming Important Dates
Book Fair 3/10 - 3/16
Go Green Week 3/13 - 3/17
Talent Show - school performance 3/21
Talent Show - Parent performance 3/22 - 6pm
Awards Assembly---March 31st----K-3rd ---9:00am
Turlock School District Test Scores Show Improvement
Here is an article from the Turlock Journal that talks about the SBAC test scores and mentions Medeiros :-)
Here is a Modesto Bee article about SBAC scores:
Character Counts!
This month the character trait is:
Trusting in one's abilities, qualities, and judgment.
Volunteer Information
If you are interested in volunteering at Medeiros, please review the following information. The Turlock Unified School District has created an interactive volunteer application. It is on their website as well as below.
Site Volunteer Packet 2016-2017 ENGLISH.pdf
Site Volunteer Packet 2016-2017 ENGLISH INTERACTIVE.pdf
Site Volunteer Packet 2016-2017 SPANISH.pdf
Site Volunteer Packet 2016-2017 SPANISH.pdf
School safety has been, and will continue to be, a very high priority for the Turlock Unified School District. We know that student discipline and bullying behavior are serious concerns in American public schools these days, and TUSD has taken a very proactive approach to deal with
student misconduct and bullying.
You can be assured that we take all reports of aggressive behavior or bullying very seriously and take appropriate disciplinary action according to these anti-bullying protocols. You can read more about these anti-bullying and safe schools procedures by accessing our District web site at www.turlock.k12.ca.us. Both students and parents can contact the confidential "Hotline" at, Student Speaking Out, 209-521-4636. This Hotline is available 24 hours, seven days a week.
If you have questions about these policies, please contact the principal at your school.
Thank you for your understanding and support for the Turlock Unified School District.
La seguridad escolar ha sido, y seguirá siendo, una prioridad muy alta para el Distrito Escolar Unificado de Turlock. Sabemos que la disciplina estudiantil y el comportamiento de intimidación son preocupaciones graves en las escuelas públicas de Estados Unidos en estos días, y TUSD ha adoptado un enfoque muy proactivo para hacer frente a la mala conducta de los estudiantes y la intimidación.
Usted puede estar seguro que tomamos todas las denuncias de comportamiento agresivo o la intimidación muy en serio y tomamos acción disciplinaria apropiada de acuerdo con estos protocolos contra el acoso. Usted puede leer más acerca de estos procedimientos de escuelas seguras y contra la intimidación mediante acceso al sitio web del Distrito al: www.turlock.k12.ca.us. Los estudiantes y padres pueden comunicarse con el "Hotline" (línea de urgencia confidencial) a Student Speaking Out, 209-521-4636. Esta línea está disponible 24 horas, siete días a la semana.
Si usted tiene preguntas acerca de estas políticas, por favor póngase en contacto con el director de su escuela.
Gracias por su comprensión y apoyo para el Distrito Escolar Unificado de Turlock.
Facebook and Cyberbullying Information
If you would like to have more information about cyberbullying and how to help your student if it happens to them, please check out the link below:
Like us on Facebook!
Common Core State Standards
As part of our TUSD journey to Common Core implementation, a page has been created on our district's website to keep our school community informed. Resources and updates will be added as we collect them.
Medeiros STAR - Principal's Message
Medeiros Star - Principal's Message for March 2017
Medeiros Star - Principal's Message for September 2016
Medeiros Star- Principal's Message for November
Medeiros Star -Principal's Message for December
Medeiros Star - Principal's Message for January/February
School Accountability Report Card
To review the 2015-2016 School Accountability Report Card for this school:
Medeiros School SARC 2015-16 - English
Medeiros SARC 2015-16 - Spanish
*Hard copies are available upon request
Medeiros Elementary School provides a free public education for all students
Section 5 of Article IX of the State of California Constitution guarantees students a "free public education." In effect, all educational activities carried on by public school districts, extra curricular, must be without cost to the students who participate in such activities. Thus, no mandatory fees will be charged for participating in but not limited to such extra - curricular activities as drama, music, and athletic competition. Furthermore, fees will not be charged for an activity even if the district waived fees for students that are financially unable to pay.
Turlock Unified School District prohibits discrimination, harassment, intimidation and bullying in educational programs, activities, or employment on the basis of actual or perceived ancestry, age, color, disability, gender identity, gender expression, nationality, race or ethnicity, religion, sex, sexual orientation, parental, pregnancy, family or marital status, or association with a person or a group with one or more of these actual or perceived characteristics. TUSD requires that school personnel take immediate steps to intervene when it is safe to do so and when he or she witnesses an act of discrimination, harassment, intimidation, or bullying.
El Distrito Escolar Unificado de Turlock prohíbe la discriminación, el hostigamiento, la intimidación y el acoso en los programas educativos, actividades o el empleo sobre la base de la ascendencia real o percibida, edad, color, discapacidad, identidad de género, expresión de género, la nacionalidad, la raza o el origen étnico, la religión, el sexo , orientación sexual, los padres, el embarazo, la familia o el estado civil, o asociación con una persona o un grupo con una o más de estas características reales o percibidas. TUSD requiere que el personal escolar tome medidas inmediatas para intervenir cuando sea seguro hacerlo y cuando él o ella es testigo de un acto de discriminación, hostigamiento, intimidación o acoso.


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Women's Opinions on the Offer and Use of Maternal Serum Screening
Roelofsen, E. E. C.
Kamerbeek, L. I.
Tymstra, T. J.
Beekhuis, J. R.
Mantingh, A.
Bibliographic Citation
PRENATAL DIAGNOSIS 1993 August; 13(8): 741-747
MetadataShow full item record
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Showing items related by title, author, creator and subject.
Maternal serum screening for Down syndrome -- opinions on acceptance from Swedish women Crang-Svalenius, Elizabeth; Dykes, Anna-Karin; Jorgensen, Connie (2003-03)
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Rheology and the Microcirculation
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activity Amer anat animals arterial arterioles Author's address Basel/New York 1969 betahistine blood flow blood vessels blood viscosity cerebral changes circulation clinical coagulation concentration Conf constriction decrease deformation dextran dilution dogs dose effect endothelium endotoxin erythrocytes experimental experiments fibrin fibrinogen fibrinolysis figure flow rate fluid hamster hematocrit hemorrhages heparin histamine hypertension increase infusion injection intravascular intravenous ischemia Karger LMWD lung lymph measured membrane method mg/kg micro microcirculation microcirculatory microscope microvascular molecular weight dextran nerve normal observed occlusion oxygen patients perfusion peripheral permeability Physiol plasma viscosity platelet platelet aggregation platelet count protein pulmonary rabbit reactivity red blood cells red cell red cell aggregation rheological Rheomacrodex saline samples serum shear rate showed skin solution suspensions Table technique thrombin thrombosis thrombus tion tissue values vascular vasoconstriction veins velocity venous venules vessel wall viscometer vitro vivo volume whole blood


text: Accessibility and inclusion Home / Community / Accessibility and inclusion Page ContentBy 2027, 1 in every 4 Australians will be living with some form of disability. The City of Stirling is committed to creating a diverse, inclusive, accessible and engaged community. iVoteThe WA Electoral Commission has launched iVote (Accessible online voting for eligible electors). iVote is a form of voting using the internet or touch tone telephone. The Commission has introduced iVote so that eligible electors can cast their vote unassisted and in secret from home or wherever they are comfortable. An eligible elector is anyone unable to vote without assistance and/or anyone who may have difficulty casting a vote at a polling place. An eligible elector will be asked to make a self-declaration, confirming their eligibility.For further information about iVote visit www.elections.wa.gov.au/ivote Accessible Stirling AwardsThe City of Stirling has recently launched the Accessible Stirling Awards in recognition of businesses within the City of Stirling who facilitate improved access and inclusion for their staff, customers or the community.Access and inclusion initiatives may include things such as providing large print menus or installing an automatic door opener which can result in increased participation of community members including people with disability, their families, friends and carers, seniors and parents with young children.Anyone can submit a nomination – customers, community members, other businesses, employees or businesses may self-nominate for an award.For more information and a nomination form please download the Accessible Stirling Awards brochure below, or contact the City of Stirling Customer Service on (08) 9205 8555 . Accessible Stirling AwardsThe City of Stirling recently recognised Westfield Innaloo with an 'Accessible Stirling' award in recognition of their efforts to facilitate improved access and inclusion for their customers.Westfield Innaloo introduced electric power shoppers and wheelchairs to provide customers with mobility issues or health problems the ability to navigate through the shops with ease. Customers that have difficulty walking to the customer service desk to access these mobility devices, have the option for a Westfield Innaloo staff member to meet them at their car.Photograph: Nikkie Dyer Concierge Supervisor at Westfield Innaloo with former Paralympian swimmer and City of Stirling resident, Elizabeth Edmondson with Mayor Giovanni Italiano.Good Access is Good BusinessA business that is accessible and inclusive increases your customer base. It benefits people with disability, seniors, parents with prams, people from culturally and linguistically diverse backgrounds and your employees. The City has developed a guide to support initiatives that help create an accessible business. This is available for download below:Accessible Stirling Guide for Businesses Recharge SchemeThe City of Stirling has partnered with RECHARGE Scheme Australia Limited to provide power points enabling people to 'top up' the battery charge on their electric wheelchairs or gophers. We are asking for expressions of interest from businesses and organisations to partner in offering this service. For more information on the scheme, please visit RECHARGE Scheme or for information on becoming a program partner, contact the City of Stirling on (08) 9205 8555.Recharge points located in the City of Stirling: Dianella,Inglewood,Mirrabooka,Osborne and Scarborough LibrariesKarrinyup Shopping CentreAccess and Inclusion InitiativesExamples of recent access and inclusion initiatives achieved by the City include: Introduced website video links for many of our community services using Australian Sign Language (Auslan) with English transcriptCompleted access works at the Hamersley Community Recreation CentreAttended seniors groups and group housing facilities to provide advice and information on security and services provided by the City's Community Safety Business UnitCommenced a pilot 'community home visit program' for people that are socially isolated at homeMeals on Wheels introduced heating instructions in Braille on meal container lids for people with vision impairmentPurchased a portable pool and spa hoist for use at the Terry Tyzack Aquatic Centre with the assistance of grant fundingHosted an art exhibition to showcase a range of works from artists with disability at Osborne LIbrary and Community Centre Developed a 'Reasonable Adjustments Management Practice' The Summerset Arts Festival 2016 presented three shows with Auslan interpreting Implemented a new 'Job Shop' initiative at the Osborne Community Centre to support City residents in their search for employment. The service includes resume support, coaching in interview techniques and general information around employabilityOpening the Yokine inclusive play spaceIntroduced an online system for dog and cat registrationsOptimised the use of electronic access for 'development applications'Provision of assistive equipment such as portable hearing loops available at key City locationsOngoing upgrades for pedestrian access and the continued implementation of the strategic footpath network delivering up to 80 footpaths per yearCreation of a virtual tour of the Mt Flora regional museumImproved access to City buildings through the development of a disability access work program Access and Inclusion Plan 2015–2020The City has developed the Access and Inclusion Plan 2015-2020 which addresses: Access to the City's services and eventsAccess to buildings and facilities owned by the City of StirlingReceiving information in appropriate formatsA high standard of service for all peopleMaking complaintsParticipating in public consultationEmployment and volunteer opportunitiesThis plan encourages contractors and businesses in the City to move towards applying the principles of access and inclusion, making the City of Stirling truly accessible to the whole community.The current Access and Inclusion Plan 2015-2020 is available to download (see below) or if you would like to request a copy in an alternative format, please contact the Stirling Customer Service Team on (08) 9205 8555. Access and Inclusion Plan 2015 - 2020Disability Advisory Panel The City has a Disability Advisory Panel (DAP) who provide expert advice and guidance to the City Officers and Council in respect to disability services matters relating to the City of Stirling. Members are drawn from organisations operating in the disability services field who have operations within the City of Stirling and from individuals residing within the City, who in the City's view, are able to contribute expertise and advice to disability services issues. Working at the CityThe City embraces diversity and encourages applications from people with disabilities. We are committed to making reasonable adjustments to provide a positive, barrier-free recruitment process and a supportive workplace. If you have any support or access requirements, we encourage you to advise us at the time of application. We will then work with you to identify the best way to assist you through the recruitment process. Services Find out about services and facilities provided by the City of Stirling. Here you can find information on services for families, women, men, seniors, and people with disabilities including Home and Community Care Services (HACC)Go to Services Beach WheelchairA Beach Wheelchair is available to the Public free of charge from the City of Stirling's Beach Services, located at the Scarboro Surf Life Saving Club. Bookings can be made during normal office hours, Monday to Friday by contacting the City's Beach Services on (08) 9205 7470. For further information please read Conditions of Use, Safety Guide and Hire Agreement. COS Beach Wheel Chair Conditions of UseCOS Beach wheelchair Hire AgreementCOS Beach wheelchair user and safety guidePortable Pool HoistThe City of Stirling was successful in obtaining a $21,770 Community Infrastructure Grant in 2015 to purchase a portable pool hoist for the Terry Tyzack Aquatic Centre in Inglewood.These grants were offered by the Disability Services Commission in partnership with the Western Australian Local Government Association. The purchase of the portable pool hoist enables hoist access to the 25 metre indoor lap pool and the 50 metre outdoor pool. It also supports one of the key strategies in the City's Access and Inclusion Plan 2015-2020, 'People with disability have the same opportunities as other people to access the buildings and other facilities of the City of Stirling'. The City anticipates that the provision of a portable pool hoist at the Terry Tyzack Aquatic Centre will result in an increase in the use of the facility by people with disability for improved health and wellbeing. Paralympian, Elizabeth Edmondson photographed with TTAC lifeguard. Translating and Interpreting ServiceThis service is designed to ensure that non-English speakers are not disadvantaged and can access City of Stirling services equitably. This service is free for non-English speaking residents and ratepayers on issues such as rates, garbage collection, community support, planning and building permit matters.For language assistance please contact the Translating and Interpreting Service (TIS) on 131 450 and ask them to contact the City of Stirling Customer Service on (08) 9205 8555. Auslan Translating ServiceAuslan is the visual language of the Australian Deaf community. Sign Language Communications Western Australia provides Auslan interpreting for people who are deaf or hard of hearing. The City offers this interpreting service free for residents and ratepayers on issues such as rates, garbage collection, community support, planning and building permit matters.For the assistance of an Auslan Interpreter, please contact the City of Stirling Customer Service on (08) 9205 8555 or via email email@example.com National Relay ServiceThe National Relay Service (NRS) can help people who have difficulty hearing or speaking on the phone by involving a relay officer in the call and in most cases you typing or reading using a special phone called a TTY, or by internet relay on a computer. See the National Relay Service website for more information or contact the NRS Helpdesk (9.00am - 5.00pm Sydney time) on 1800 555 660. Creating an accessible event.The City supports accessible events that can be enjoyed by all people including people with a disability, people with prams and young children, many older people, people making deliveries and people with heavy baggage. An event such as an exhibition, conference, fundraiser, Annual General Meeting, outdoor concert, street fair or award ceremony are an important part of the lives of many people.If you are a person or organisation hosting an event, you have a legal responsibility to avoid discrimination and remove barriers to equal access. Have you considered accessible parking close to the event and toilets with enough room for a person using a wheelchair? For information on how to create an accessible event, a guide has been developed by the Meetings and Events Industry of Australia in partnership with the Human Rights and Equal Opportunity Commission. This guide can be found at Accessible EventsThe Disability Services Commission – Local CoordinationThe Disability Services Commission (DSC) has two Local Coordination Offices in the City of Stirling. Local Coordinators (LCs) have local knowledge and can assist people with disability by providing information and helping them to plan, organise and access supports and services. LCs also work with local communities to be more inclusive and welcoming. For information visit the DSC website or contact local offices using the information below:Mirrabooka Office:2 Cobbler Place, Mirrabooka Phone (08) 9440 4898 Innaloo Office:16 Morris Place, Innaloo Phone (08) 9446 6785AdvocareAdvocare offers a free, professional and confidential advocacy service throughout Western Australia empowering people through advocacy, information and education. To speak to an Advocate please contact Advocare Incorporated during office hours, 8.30am-4.30pm Monday - Friday on (08) 9479 7566 Can't Wait Card Crohn's Disease and ulcerative colitis are inflammatory conditions where the lining of the one or more parts of the gastrointestinal tract become inflamed. It affects over 75,000 Australians most commonly diagnosed in people aged between 15 – 30 years. They are chronic conditions with no cure and the cause is unknown. The City of Stirling show their support for people with the medical condition Crohn's and colitis, collectively known as inflammatory bowel disease (IBD), by recognising the Can't Wait Card at participating City of Stirling facilities. The Can't Wait Card is issued to members of Crohn's and Colitis Australia (CCA) who have been formally diagnosed with Crohn's or Colitis (IBD), to help them gain access to a toilet in times of urgency. To find a participating venue to go the Cant Wait website To learn more about Crohn's and Colitis go to the Crohns and Colitis website 'You're welcome' projectPeople with disability, carers and seniors can search to find accessible business and community facilities in the City of Stirling. Access information will assist people of all ages and abilitiesFind access details of businesses and attractions in the City of Stirling and Western Australia such as accommodation and cafes. Check out diagrams of toilets and bathrooms to choose what suits you bestUsed 'advanced search' to find accessible accommodation, toilets and parking to meet your particular needsVisit the Access WA website for more information.


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A love story with typical turns on the lines of Godavari.
Director Vamsi, who gave big hits like Sitara, Anveshana, Manchu Pallaki, Ladies' Tailor and Avunu Valliddaru Ishtapaddaru, is almost running into bad time with successive failures like Donga Ramudu and Party, Koncham Touchlo Vunte Chebutanu and Anumanaspadam at the Box Office.
In order to regain his past glory, Vamsi has brought out another film silently with not so famous actors, again with his favourite Godavari and the boats in the backdrop. The entire film shooting was done in a low profile. The movement of the film is exactly in Vamsi's style and the film is good to watch but the slow running of the flick may not suit the taste of the present generation audiences.
Gopika (Kamalini) is a doctor. She runs a floating hospital on river Godavari. The boat tours the entire coast of the river and serves the patients. The running of the hospital is being taken care of and funded by an NGO. One Shyam (Kalyan), who has completed his MS in the US learns about the hospital and reaches India to run a similar service near Srisailam. Shyam loses his heart at first sight to Gopika. But the latter refuses his marriage proposal.


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Print advertisment created by Creative Child Studios, Brazil for WWF, within the category: Public Interest, NGO.
Put your shirt and join to our team.
Creative Director: Fabio Bispo
Art Director: Eliayse Villote
Photographer: Dreamstime
Highest Rated
"Let's put oil on these penguins in the shape of a panda..."
That's a very clumsy attempt...
Brand Design | Logo Design | Brochure Design | Website Design | Branding Packages
Not bad but old concept about t-shirt
"Let's put oil on these penguins in the shape of a panda..."
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The Bora Bora is a small hostal consisting of 14 modern rooms situated just 7 minutes walking distance from the Las Canteras Beach and 3 minutes from the main market.The hostal underwent a refurbishment this year and has a reception, lounge with 46inch TV, free wifi, conferernce room, bike and equipement storage and a small terrace.
Located in the heart of Las Palmas - ideal for the beach and shops, markets and restaurants along with all the amenties of a large bustling capital.Ideal for those looking for a holiday with a more local feel away from the usual tourist resorts.
Small hostal in a great location just 7 minutes from the beach
* Reception


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AWBeautician- Beauty Clinic
Bruntsfield, Edinburgh
Our AWBeautician Beauty Clinic offers a range of beauty treatments including waxing,manicures & pedicures,facial treatments,body treatments We are advanced specialists Endermologie LPG ,Laser hairs removal,Hollywood waxing.Come along to one of the best salon in Edinburgh to tray it!
Endermologie LPG Anti -Cellulite & Slimming Body
35 mins
LPG-Endermolift Anti-Aging treatments
30 mins
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Charisma Studio
Knightswood, Glasgow
Taking care of you from hair to toenails is the friendly Carisma Studio, a skin clinic, waxing and hair salon with a unique style in Glasgow's Knightswood Area. Open 7 days a week this is probably the cutest wee hair and beauty salon you'll ever go to. Chunky wood panelling meets exposed brick and patterned wallpaper, all blended together to create a uniquely relaxing atmosphere. The quirky interior is certainly eclectic but has the charisma to pull it off with charm. However, it's the Scottish Hair and Beauty, award winning staff that make Carisma special. The team really focus on providing a friendly customer experience and great treatments. Their service list is as varied as the decor and has more than enough on offer to leave your whole body feeling refreshed. Popular services include Balayage, deep tissue massage, Hollywood waxing and semi-permanent makeup. But there are other deliciously decadent pampering options on offer that sound good enough to eat, like their chocolate therapy body wrap... mmm! Whatever the occasion, when it comes to scrubbing up well, you're going to want to do it with Charisma Studio.
Use it N Lose it
1 hr
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AU Beauty
Finnieston, Glasgow
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text: RPG Information Edit Hands are Axes (Tasaii-do emphasis) Hands are Axes allows the advanced practitioner of a Tasaii-do to increased the damage when performing an unarmed attack or using a bo. [1] References ↑ Way of the Open Hand Web Supplement, p. 7 Retrieved from "http://l5r.wikia.com/wiki/Hands_are_Axes?oldid=176663" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Advanced Maneuvers Add category Cancel Save


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Gateway DX4300-22 Refurbished Desktop PC - AMD Phenom II X4 810 2.6GHz, 6GB DDR2, 1TB HDD, DVDRW, Windows 7 Home Premium 64-bitItem#:� S445-10076 �|�Model#:�RB-L-PT.G8302.008 �|�
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The terms refurbished and recertified are used interchangeable to describe products that manufacturers refurbished, inspected, brought back to original manufacturer�s specification then tested and repackaged for sale. Some manufacturers refurbish products themselves and others use a qualified third party refurbisher. Refurbished products represent a great value and we offer them with confidence knowing that every refurbished product was tested at least twice, once when it was first manufactured and again during the refurbishing process. Refurbished products are warranted by either the original manufacturer or the manufacturer�s third party refurbisher. Warranty terms applicable to refurbished products may vary; see below for specific warranty information and consider purchasing an extended service plan if you desire even more coverage.
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Pam M's Profile
I am married to a wonderful man who likes to look at my latest creations. We live in Florida and love it here. We had our 1st child a girl in April of 2009 Our 2nd a boy was born in June 2012. I enjoy sewing and quilting among other hobbies. We opened a retail Fabric Store in Nov 2013 where we sell all quality quilting fabric and supplies
I hope you enjoy my items. Thanks.
Please note the name change from Pams Crafting Place to Pam's Fabric Nook. I am still the same just a new name.
Here is my video advertisement that will be shown on HGTV and DIY Network by Comcast on April 14, 2014. https://www.youtube.com/watch?v=B5eBERS3jIw
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MATERA + is an ERA-Net Plus project co-financed by the Seventh Framework Programme of the EU aiming at launching, managing and following-up a Joint Call for Proposals for research projects in the field of Materials. This ERA-NET Plus project on Materials Research was built upon the experience in development of joint calls and other cooperation activities gained in MATERA.
MATERA+ Call was launched on March 2009 with financial resources from the European Commission and 20 national and regional Funding Agencies from 14 different countries.
Following the results of an international evaluation by independent experts, 20 trans-national projects were selected with 15 Million Euros funding.
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JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
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This page is for rumors about official games and these rumors must be proven with evidence (i.e. pictures, screenshots, links, etc.). Enjoy! --Metallix-117R 06:19, March 28, 2010 (UTC)
Kingdom Hearts Birth by Sleep
I have reason to believe that the rumors of Kingdom Hearts Birth by Sleep not being offered as downloadable on the PSN are false and these set of screen shots taken by me prove it.
Proof & Truth
If you look at


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Travis arrived for his interview with quite a load saved up. He has been celibate for seven long months. Being a dancer has helped to work off some his sexual frustration, but not all of it. During their initial chat, it was quite obvious he had more on his mind than a jack off session. Tony, who was conducting the interview, asked Travis if he wanted to do a bit more than was on the menu. After abstaining for seven months, who wouldn't?
And why shouldn't Tony make that offer to Travis? Here is this trained dancer, who is gay with a fine toned body. Though not a bodybuilder, his physique is just as delicious. Once Tony got in front of the camera, Travis immediately reached for his cock. With a wide smile, he quickly gobbles up Tony's cock. After seven months, you can tell Travis is quite hungry. Sucking Tony's dick long and deep. Did we mention that oral is Travis favorite thing to do?
After finally coming up for air, Travis turns his attention to Tony's balls. Simultaneously he also begins to stroke Tony's cock. During this suck-a-thon, Travis peers up at Tony occasionally to gauge his approval. Wanting to satisfy his own hard-on, Travis pulls down his pants and starts to jack off his own uncut cock. Changing positions, Travis lays back and continues to jerk off while slurping on Tony's sack.
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My DD is 2.5 and has been out of dipes for a week or so and is only wearing undies or Flip trainers. She is most consistent with pee and is having a poop accident every other day or so (I think the Flip trainers feel too much like dipes to her and is confusing). What I am wondering is how I should deal with the trainer inserts and undies that fell victim to the poop accidents, now that I am no longer doing a whole diaper washing routine. Do they get tossed in with the laundry? It seems like such a waste of water to run a cycle with just 2-3 things in it.
x-posted in EC and toddlers
Mama to a sweet little redheaded girl, born 11/09; and my HBAC baby, little brother, born 7/12.
When I've had just a few accident undies/trainers -- I would soak them (often with some Dr.Bronner's TTO soap, or regular liquid detergent), rinse well, and toss them in with towel/rags laundry.
I think just washing them out beforehand and then tossing them with regular laundry is fine. Especially if it's mostly poop accidents. The main reason you don't wash diapers with regular laundry and detergent is because it will cause them to lose absorbency so that's not the big issue.
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K-os Exit (full album) MP3
i don't take any claim to any of this,,,,,
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K os Patience.
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Could only find ones from Anchorman mixtape with lines at the beginning. cut them out. No copyright infringement intended, just getting a better version out there ...
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Spaceship" performed by k-os. Taken from the album Can't Fly Without Gravity. WATCH THE MUSIC VIDEO: https://youtu.be/R7CEh5kizx4 AVAILABLE NOW ...
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It's a classic. Subscribe.
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from joyful rebellion.
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Heard this song on the radio. then i noticed no one had it up on youtube. credit goes to the artist and the lable. this video is purely for entertainment purposes ...
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K-os: Freeze MP3
K-os: Freeze Lyrics: Yo, yo what the fuck, yo, yo ***Chorus*** It goes stop, don't make a move, just freeze Instead of tellin' a lie, get down on your knees Please, ...
K -os - Crabbuckit MP3
K-os - Crabbuckit.
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From the Album: Yes! https://itunes.apple.com/ca/album/yes!/id311262604 http://www.k-osmusic.com https://www.facebook.com/kosmusic ...
k-os - Crucial (Live at the Edge) MP3
k-os performs "Crucial" at 102.1 the Edge's Sugar Beach studio in Toronto. April 3, 2014. Camera & Edit: Chris Santos Recording: Michael Emmons Mix: Adam ...
[MP3] [Girl K OS ] FORGET ABOUT ME - Kim Jung Tae MP3
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text:
Farnesylation or geranylgeranylation? Efficient assays for testing protein prenylation
We describe a new technique for detecting prenyl anchors in N-terminally glutathione S-transferase (GST)-labeled constructs of target proteins expressed
Savings in time for experimentation and the higher sensitivity for detecting 3H-labeled lipid anchors recommend the TLC-scanning method with purified GST- (or HA-) tagged target proteins as the method of choice for analyzing their prenylation capabilities
Prenylation is a lipid posttranslational modification (PTM) of proteins at cysteine residues in the C-terminal region
Members of the Ras family of GTPases are of particular medical interest, as their mutational hyperactivation as well as mutations of proteins lying upstream in their signaling pathways are associated with various cancers
However, we are far from understanding the physiological consequences of inhibiting FTase or GGTase1 in cells since the lists of the respective substrates are essentially not known. Only a few dozen proteins, including several fungal lipopeptide pheromons
The standard literature method for
The problem of long exposure times for 3H-autoradiography has prompted us to study a variety of chromatographic and scintillation methods for developing a faster and more sensitive test system. We found a solution using a TLC linear analyzer for testing the prenylation of selected protein targets. N-terminally GST-tagged proteins were
Optimization of experimental parameters and analysis of the prenylation behaviour of the protein Rap2A
The proposed new procedure starts with a PCR-amplification of the GST-Rap2A open reading frame (Genbank accession of Rap2A
Experiments with wildtype GST-Rap2A fusion protein and [3H]mevalonic acid were performed using various reaction times and amounts of radioactive label. The optimal conditions we found were 20–40 μCi [3H]mevalonic acid and at least four hours reaction time, which is in agreement with previous studies
Experiments with 20 μCi [3H]mevalonic acid, 10 μCi [3H]farnesylpyrophosphate (FPP) and 10 μCi [3H]geranylgeranylpyrophosphate (GGPP) allowed affirmation of prenylation of Rap2A and identification of the preferred isoprenoid attached to Rap2A as a farnesyl-group. However, geranylgeranylation did also occur under the given conditions, but with much lower efficiency (Figure
Figure 1
Western blots and TLC scanning results for Rap2A with radioactive prenyl anchor precursors
All results obtained with our new method were consistent with previously reported data on Rap2A
Figure 2
Autoradiographs of Rap2A after exposure to radioactive prenyl anchor precursors
To determine the enzyme prenylating Rap2A, we performed the same assay with and without inhibitors of prenyltransferases. The signal yielded by incorporation of [3H]FPP was reduced to background level upon addition of 50 μM of the FTase inhibitor FTI-277. In addition, the already weak signal of [3H]GGPP-incorporation was diminished to background level by FTI, while application of a GGTase inhibitor (GGTI-298) left a small peak (Figure
Figure 3
Western blots and TLC scanning results for Rap2A incubated with prenyltransferase inhibitors
Analysis of the in vitro prenylation of RasD2, v-Ki-Ras2 and RhoA63L with the TLC scanning method
These three candidates have been selected to show the ability of our new technique to detect alternative prenylation modes. RasD2 (synonym: Rhes, BC013419) is suggested to be a farnesylation target but only due to indirect evidence
The same
Figure 4
Western blots and TLC scanning results for RasD2 with radioactive prenyl anchor precursors
On the contrary, while also showing preference for FPP, incorporation of GGPP into v-Ki-Ras2 in the absence of FPP is only 2–3 times lower (Figure
Figure 5
Western blots and TLC scanning results for v-Ki-Ras2 (K-Ras-4B) with radioactive prenyl anchor precursors
Figure 6
Western blots and TLC scanning results for RhoA63L with radioactive prenyl anchor precursors
Electrophoretic mobility shifts of in vivo prenylated proteins
The simplest
Clear electrophoretic mobility shifts have been observed for Rap2A providing an indirect argument for its farnesylation (Figure
Figure 7
Mobility changes of the prenylated protein form: Immunoblot analysis of Rap2A
In vivo subcellular localization of N-terminally GFP-tagged constructs
To confirm the biological relevance of the results from our
Figure 8
Localization of N-terminal GFP-constructs of Rap2A, RasD2, v-Ki-Ras2 and RhoA63L in HeLa cells
A) GFP-Rap2A, GFP-RasD2 and GFP-v-Ki-Ras2 are membrane-localized with (4, 8, 12) or without (1, 5, 9) GGTI-298 treatment. Mutation of the Cys in the CaaX box (2, 6, 10) or treatment with FTI-277 (3, 7, 11) cause mislocalization and accumulation of the fusion proteins in the nucleus.
B) GFP-RhoA is membrane localized with (15) or without (13) FTI-277 treatment. Mutation of the Cys in the CaaX box (14) or treatment with GGTI-298 (16) cause mislocalization and accumulation of RhoA in the nucleus.
Further, we investigated the subcellular localization of the GFP-v-Ki-Ras2 fusion protein in HeLa cells. As shown in Figure
In the literature, K-Ras4A and K-Ras4B have been reported to be predominantly farnesylated
Analysis of the in vivo prenylation of Rap2A with the TLC scanning method
It would be desirable to test whether the TLC scanning method is applicable also for the investigation of protein targets exposed to metabolic labelling with radioactive precursors
Figure 9
Western blots and TLC scanning results for Rap2A with radioactive FPP in vivo
Attempts to detect the prenylation status of Rap2A with HPLC-based purification methods
We have also attempted the proof of prenylation with chromatographic methods. In one of these variants, we utilized coupled
The critical step in classical prenylation assays is the detection of the radioactive anchor with autoradiography/fluorography. Unfortunately, the sensitivity of this approach is weak since the 3H-labeled anchors emit low-energy radiation and the amount of purified protein with attached anchor is typically very low before expression and modification conditions have been optimized individually for the specific target. The necessary exposure time is
Even having a negative outcome after 2 months exposure does not clarify whether the protein investigated cannot be prenylated or whether the amount of protein after purification is simply too low, for example due to unspecific adsorption to test tubes promoted by the prenyl anchor. It should be noted that the issue is not resolved with a [35S]methionine-based control of translation in a parallel experiment. However, this problem is circumvented by another advantage of the present protocol. It has the possibility to evaluate the amount of protein directly from the Western blot that has been used for TLC scanning. If a band is detected with low intensity of anti-GST- or anti-HA-antibody binding, this indicates that the expression of the target protein must be up-scaled.
Filter binding assays (i.e., the separation of proteins transcribed and translated
To our knowledge, the current method is the first one utilizing a TLC-Scanner for the analysis of putative targets of PTases on Western blot membranes. Compared to autoradiography/fluorography, this approach reduces the detection time from several weeks/months to 20 minutes per lane, resulting in an overall time effort for the whole experiment of about three days, given that the cDNA of the GST- (or HA-tag-) fusion protein is already available. Additionally, this assay detects incorporated 3H-label and translation efficiency of the same reaction, rendering control reactions with [35S]methionine redundant and reducing variability of results caused by pipetting inaccuracies. In conclusion, the TLC scanning method is more sensitive and offers a more reliable way of quantification of any covalently linked 3H-labeled posttranslational modifications in much less time compared with autoradiography. Especially, when conditions for
The use of a GST-tag (or a HA-tag) provides a way of removing free radioactive label as well as separation from highly abundant proteins from the rabbit reticulocyte (
We have shown that the results obtained with our TLC scanning method are in line with those from parallel experiments testing electrophoretic mobility shifts (Figure
Unfortunately, we were not able to find any conditions, which reproducibly allowed detection of the prenylated peptide with HPLC-based purification methods after proteolytic digest. Most of the times, there was no visible difference between wildtype and mutant Rap2A in UV-signals as well as radioactivity measurements, except the fact that the total peptide content was significantly (ca. 3 times) higher for the mutant protein. These findings suggest that, in contrast to the non-prenylated protein, a considerable amount of prenylated protein is lost by unspecific adsorption to the walls of the Eppendorf tubes, vials, tubes and microwell plates used for the reaction and following processing steps. This is in agreement with our observation from the Western blots of the TLC scanning method (Figures
These problems show that the chromatographic method might not be applicable for the small amounts of protein yielded by
We think that the mechanistic role of the prenyl anchor for the biological function of the proteins studied in this work still requires additional research. For the convenience of the reader, we summarize the current state of knowledge with respect to the molecular and cellular functions of the investigated protein targets for prenylation in the following four paragraphs. Apparently, Rap2A, RasD2, K-Ras and RhoA need the prenyl anchor to get translocated into the right signaling context by membrane association. Rap2 has been shown to promote integrin activation
RasD2/Rhes (
The Ras proteins have been reported to be involved in many signaling pathways, regulation cell differentiation and proliferation as well as cell shape and motility, to mention only the most important. Ras proteins are GTPases that function as molecular switches, being active in GTP-bound state and inactive when GDP-bound. The different Ras proteins show high homology to each other and collaborate in a complex network, making it hard to distinguish whether their functions are provided by all of them or are unique for a certain type of Ras protein. Nevertheless, there is some experimental data indicating specific functions of K-Ras4B in cell-cell and cell-matrix contacts as well as in apoptosis
In humans, there are three highly homologous isoforms of Rho GTPases, called RhoA, B and C
Savings in time for experimentation and the higher sensitivity for detecting 3H-labeled lipid anchors recommend the TLC-scanning method with GST-tagged or HA-tagged target proteins as the method of choice for analyzing their prenylation capabilities
Construct production and cloning
We generated plasmids containing GST- and pEGFP-fusions of all genes studied in this work. The cDNAs of Rap2A (IMAGE clone ID IMAGp998M0310712, Genbank accession
In vitro prenylation assay
The cDNA of the GST-fusion proteins was amplified by PCR using standard conditions. A 5'-primer has been designed especially for
The pellet was re-suspended in sample buffer, incubated at 80°C for 10 min and resolved by SDS-PAGE (15%). The protein was transferred from the gel to a nitrocellulose membrane by electroblotting. The membrane was left to dry. Each lane was scanned separately for 20 min using a Berthold TLC linear analyzer LB 282. The spatial resolution of the scanner allows to assign each signal to a certain protein size. Following this measurement, the membrane was blocked with 10% milk powder in PBS. After incubation with primary antibody (anti-GST-antibody from rabbit, 1:5000) and secondary antibody (ECL Anti-rabbit IgG, Horseradish peroxidase linked whole antibody from donkey purchased from Amersham Biosciences, 1:10.000), addition of ECL plus Western Blotting Detection solution and exposure of a Hyperfilm ECL (both from Amersham Biosciences) for 15 seconds, a band at a molecular weight corresponding to the signals measured by the TLC analyzer is detectable.
Determination of differential electrophoretic mobility after expression in cell culture
For N-terminal tagging, the ORFs of Rap2A (wild-type) and the Rap2A C180A mutant form were cloned into the plasmid pCIneo-HA
Ca. 16 hours later, extracts were prepared with lysis buffer (50 mM Hepes, 140 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) Sodium Deoxycholic acid, Complete Protease Inhibitor Cocktail (Roche)). Before loading the samples onto a 16% SDS Gel, the extracts were centrifuged for 10 minutes at 13000 rpm using a table top centrifuge and the supernatants boiled with sample buffer for 5 minutes. The proteins were transferred to nitrocellulose membranes and probed with mouse anti-HA 12CA5 antibodies and HRP-conjugated secondary antibodies.
Determination of intracellular localization
HeLa cells were plated at low density on coverslips. Then, they were transfected with GFP-expression vectors carrying the cDNA's of Rap2A, RasD2, v-Ki-Ras2 and RhoA63L using Lipofectamine and Plus Reagent in serum-free medium (Life Technologies) for 3.5 h. After washing, the cells were maintained in growth medium for 14 h. Cells were rinsed with PBS, fixed in 2% formaldehyde in PBS for 20 min, washed with PBS, permeabilized with 0.1% Triton X-100 in PBS for 10 min, washed with PBS and mounted in vectashield (vector laboratories) for direct fluorescence of GFP. The effect of farnesylation or geranylgeranylation inhibitors was assessed by treatment of the cells with FTI-277 (10 μM) or GGTI-298 (5 μM) (Sigma) during maintenance in growth medium for 14 h. Cells were observed using an Axiplan 2 Imaging Microscope (Zeiss). GFP- as well as DAPI-images were acquired with a Coolsnap HQ camera (Photometrics) and analyzed using the software Metamorph 6.2r4 (Universal Imaging Corp.).
In vivo prenylation assay with HA-tag-based immunoprecipitation from cell culture and Western blot TLC scanning
Transfection and labelling
For N-terminal tagging, the ORFs of Rap2Awt and Rap2AC180A were cloned into the plasmid pCIneo-HA
After ca. 16 hours, extracts were pre-cleared with Dynabeads M-280 Sheep anti-Mouse IgG (Dynal) in lysis buffer (50 mM Hepes, 140 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) Sodium Deoxycholic acid, Complete Protease Inhibitor Cocktail (Roche)) at room temperature for 3 hours. Immunoprecipitation was performed with mouse anti-HA 12CA5 antibodies crosslinked to magnetic Dynabeads M-280 Sheep anti-Mouse IgG (Dynal) at 4°C over night. We washed the beads 3 times with lysis buffer and twice with lysis buffer containing 500 mM NaCl. Before loading the samples onto a 10 % SDS Gel, the beads were boiled in sample buffer for 5 minutes. The proteins were transferred to nitrocellulose membranes and monitored for incorporation of 3H-FPP anchors by TLC analysis. Subsequently, we probed the Western blots with mouse anti-HA 12CA5 antibodies and HRP-conjugated secondary antibodies. Magnetic beads were washed three times with TBS-T (0.01% Triton X-100) and incubated with mouse anti-HA 12CA5 crude serum at 4°C over night.
Crosslinking to beads
The beads were again washed 3 times with TBS-T and 3 times with 0.2 M Sodiumborate pH 9.0. We crosslinked beads and antibodies with 20 mM DMP in 0.2 M Sodiumborate pH 9.0 for 15 minutes at room temperature. Finally, the beads were washed 3 times for 15 minutes with 1 M Tris pH 8.0 and 3 times with TBS-T.
FPP, farnesylpyrophosphate; FTase, farnesyltransferase; FTI, farnesyltransferase inhibitor; GAP, GTPase activating protein; GEF, guanine nucleotide exchange factor; GFP, green fluorescent protein; GGPP, geranylgeranylpyrophosphate; GGTase1, geranylgeranyltransferase 1; GGTase2, geranylgeranyltransferase 2; GGTI, geranylgeranyltransferase inhibitor; GST, glutathione-S-transferase; HA, hemagglutinin; PBS, phosphate-buffered saline; PCR, polymerase chain reaction; PTases, prenyltransferases; PTM, posttranslational modification; SDS-PAGE, sodium dodecyl sulphate polyacrylamide gelelectrophoresis; TLC, thin layer chromatography
Authors' contributions
FE initiated this research. The experiments were performed by WB and MK. WB, MK, FP and FE conceived the work and advised modifications of experimental designs. The sequence-analytic part was carried out by SMS. WB, MK and FE contributed to the writing of the manuscript and all authors read and approved the final text.
The GFP-RhoA plasmids were kindly provided by Channing J. Der (University of North Carolina, USA). The authors received support in methodical questions of detection of lipid-modified proteins with HPLC and mass-spectrometry by Mike Ferguson, Nick Morris, Douglas Lamont and Kenny Beattie (University of Dundee). Help in establishing the biochemical laboratory was provided by Gustav Ammerer, Andreas Hartig, Franz Koller, Wolfgang Löffelhardt and Wolfgang Reiter (University Vienna) as well as Kim Nasmyth, Barry McGuinness, Thorsten Decker, Gunnar Schotta, Kerstin Wendt, Peter Steinlein and Visnja Pavicic (IMP Vienna). Josefina Pinon is thanked for editing the manuscript. The authors are grateful for financial support from Boehringer Ingelheim. Chromatography equipment was generously provided by Agilent Technologies Vienna (with support from Franz Weigang). This project has been partly funded by the Austrian Gen-AU bioinformatics integration network (BIN) sponsored by BM-BWK (grant to FE).
Prenyl proteins in eukaryotic cells: a new type of membrane anchor
Sequence dependence of protein isoprenylation
Substrate characterization of the Saccharomyces cerevisiae protein farnesyltransferase and type-I protein geranylgeranyltransferase
Protein prenylation: molecular mechanisms and functional consequences
Protein prenyltransferases
Protein prenyltransferases: anchor size, pseudogenes and parasites
Refinement and prediction of protein prenylation motifs
Prenylated proteins: the structure of the isoprenoid group
Role of protein modification reactions in programming interactions between ras-related GTPases and cell membranes
Farnesylated gamma-subunit of photoreceptor G protein indispensable for GTP-binding
Prenylation-dependent interaction of Rab proteins with GDP dissociation inhibitors
Prenyl-binding domains: potential targets for Ras inhibitors and anti-cancer drugs
The effect of posttranslational modifications on the interaction of Ras2 with adenylyl cyclase
The geranylgeranyl moiety but not the methyl moiety of the smg-25A/rab3A protein is essential for the interactions with membrane and its inhibitory GDP/GTP exchange protein
Prenylation of Ras proteins is required for efficient hSOS1-promoted guanine nucleotide exchange
Efficient interaction with a receptor requires a specific type of prenyl group on the G protein gamma subunit
All ras proteins are polyisoprenylated but only some are palmitoylated
p21ras is modified by a farnesyl isoprenoid
The ras/cholesterol connection: implications for ras oncogenicity
ras oncogenes in human cancer: a review
RAS oncogenes: the first 30 years
Creation of human tumour cells with defined genetic elements
Cell signaling by receptor tyrosine kinases
The discovery of receptor tyrosine kinases: targets for cancer therapy
Rho GTPases in transformation and metastasis
RHO-GTPases and cancer
Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice
Identification of amino acid residues required for Ras p21 target activation
Post-translational modifications of the C-terminal region of the rho protein are important for its interaction with membranes and the stimulatory and inhibitory GDP/GTP exchange proteins
Isoprenoid modification and plasma membrane association: critical factors for ras oncogenicity
Isoprenoid addition to Ras protein is the critical modification for its membrane association and transforming activity
Selective inhibition of ras-dependent transformation by a farnesyltransferase inhibitor
Farnesyltransferase inhibitors: Ras research yields a potential cancer therapeutic
Inhibition of farnesyltransferase induces regression of mammary and salivary carcinomas in ras transgenic mice
Perspectives on farnesyl transferase inhibitors in cancer therapy
Farnesyltransferase inhibitors as anticancer agents: critical crossroads
Identification of a prenylation site in delta virus large antigen
Structure of rhodotorucine A, a novel lipopeptide, inducing mating tube formation in Rhodosporidium toruloides
Fungal lipopeptide mating pheromones: a model system for the study of protein prenylation
Evidence for an S-farnesylcysteine methyl ester at the carboxyl terminus of the Saccharomyces cerevisiae RAS2 protein
Total in vitro maturation of the Saccharomyces cerevisiae a-factor lipopeptide mating pheromone
Evidence for post-translational incorporation of a product of mevalonic acid into Swiss 3T3 cell proteins
Heterotrimeric G-protein betagamma-dimers in growth and differentiation
Nucleoplasmic localization of prelamin A: implications for prenylation-dependent lamin A assembly into the nuclear lamina
Human lamin B contains a farnesylated cysteine residue
Prediction of sequence signals for lipid post-translational modifications: insights from case studies
Reticulocyte lysate assay for in vitro translation and posttranslational modification of Ras proteins
Coupled translation/prenylation of Rab proteins in vitro
Palmitoylated cysteine 192 is required for RhoB tumor-suppressive and apoptotic activities
Prenyl group identification of rap2 proteins: a ras superfamily member other than ras that is farnesylated
Differential prenyl pyrophosphate binding to mammalian protein geranylgeranyltransferase-I and protein farnesyltransferase and its consequence on the specificity of protein prenylation
The small GTP-binding protein, Rhes, regulates signal transduction from G protein-coupled receptors
K- and N-Ras are geranylgeranylated in cells treated with farnesyl protein transferase inhibitors
The posttranslationally modified C-terminal structure of bovine aortic smooth muscle rhoA p21
RhoA biological activity is dependent on prenylation but independent of specific isoprenoid modification
Inhibition of the prenylation of K-Ras, but not H- or N-Ras, is highly resistant to CAAX peptidomimetics and requires both a farnesyltransferase and a geranylgeranyltransferase I inhibitor in human tumor cell lines
Differential prenylation of proteins as a function of mevalonate concentration in CHO cells
In Vitro Prenylation of the Small GTPase Rac13 of Cotton
The Rap GTPases regulate integrin-mediated adhesion, cell spreading, actin polymerization, and Pyk2 tyrosine phosphorylation in B lymphocytes
Interaction of the low-molecular-weight GTP-binding protein rap2 with the platelet cytoskeleton is mediated by direct binding to the actin filaments
Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade
Rhes: A striatal-specific Ras homolog related to Dexras1
Identification of the monomeric G-protein, Rhes, as an efaroxan-regulated protein in the pancreatic beta-cell
Rhes is involved in striatal function
Ras and relatives--job sharing and networking keep an old family together
The importance of being K-Ras
Rho family proteins: coordinating cell responses
Rho GTPases in cell biology
Rho GTPases and the actin cytoskeleton
Histone deacetylase 1 can repress transcription by binding to Sp1


text:
Would you like to save as your currency permanently or for this visit only?
Checked in early with no problem.
The ease of getting to your rooms. The breakfast was fantastic (except for the scrambled eggs....bad....I think they were powdered eggs or frozen that they just heated up). The cookies they had for a snack in the afternoon. The bed and pillows were good The AC system. Very loud every time it clicked on....on both sides of the room. The thermostat clicked loud going on and then the unit at the window was loud going on. Wakes you up at night. other than that....a great room!
This guest did not leave comments about this hotel.
Terrific breakfast
Clean and convenient Had strong odor in bathroom
Some of the front desk personnel were not as friendly as others, but overall we had a very positive experience. The WiFi was incredibly slow.
The room was clean and perfect. The breakfast was the most amazing hotel breakfast we've ever had. Can't wait to come back! Wish the indoor pool was heated. Way too cold to enjoy!
I was in the area for a family wedding. The location was convenient. The staff was friendly & knowledgeable about the area. The room was quiet & very clean.
Great !! Friendly staff hotel was very nice well kept. Nice rooms very comfortable beds. Clean and modern decor though out hotel. Great location for us. Love the breakfast set up in the morning wonderful selection.great having coffee available 24 hours Only complaint is the indoor pool is NOT heated !! It's like and ice bath !!! What's the point of an indoor pool if it's freezing cold. You can't enjoy the pool they warn the room temperature to make up for the freezing pool which does not help.
Generous breakfast with lots of good fruit and pasteries. Unfortunately the 'eggs' were made from a powder and it made the rest of the breakfast seem a little cheap.The location is great, very convenient They advertised a heated, indoor pool. This is one reason we chose it. But when we checked in we found out it was not useable. There was no attempt to appologize, much less compensate.
It was clean and close to where I needed to be. The pool was not available and apparently, under repair. However, I was not made aware of that ahead of time. The wireless was weak and unreliable - kept dropping.


text:
|By Libro
|More from Libro
|Action, Comedy
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|Gameshow of Horror - Part 2: The Extreme Laser
|Chapter information
|Written by
|Release date
October 22, 2015
|Last chapter
|Next chapter
Fire, Air, Water, Earth. Someday, The Lost Earthbender will balance to the world.
Jinora, Asami, Chang, and Luli ran around the station to find Jing and Bo. Jing meanwhile managed to earthbend everyone out, the walls were made of metal, which Jing did not know how to metalbend.
"It's no use, Jing. You cannot get out," said Anna.
"I'm getting out, because I'm helping you," replied Jing.
Jing continued trying to escape, but then big brother made a statement.
"This is Big Brother. Jing will be ejected, because he has broken several rules. Jing, please leave the Big Brother House."
"Yes! Let's get out!" said Jing.
"You must be joking, you're going to die," replied Derek.
Big Brother got ready for the laser, which would kill Jing. However, Jing went into the Avatar State and broke the laser with earthbending.
"Told ya! I'm getting out! Come with me, Anna!" said Jing.
Anna joined Jing to find his friends, and Derek went his separate ways. Meanwhile, another person is lasered in Bo's game. Bo became more scared.
Bo asked Wellard, "Why are we getting rid of the good ones?"
Wellard replied, "Because you're the dumb one."
"Well?" Bo said angrily.
"Ard... Get it? Ha ha ha!" said Wellard.
AN.N prepared Wellard and Bo for the final round of the game. Wellard began getting the correct answers, while Bo was struggling. Jing and Anna managed to find Jinora and the others.
"I found you! Where's Bo?" said Jing.
"We don't know. We have to find her quickly before she dies!" replied Luli.
"Let's split up," said Jinora.
"No, that would not be a good idea. You could be caught, we have to find her together," said Jing.
Jing and his friends ran around to find Bo before it was too late. They bumped into a room which had all the names of those currently playing the games. They searched for Bo's name.
"Oh no! AN.N is the worst to be lasered! Quick, follow me!" said Anna.
Everyone followed Anna. Bo had one last question, it was very critical.
"If you get this question wrong, sweetheart, you will die! MUHAHAHAHA!" said AN.N.
Bo was scared, and the question was, "Which nation holds Avatar Wan Day?" Bo did not know, and she stared at the AN.N's red eyes. She took a deadly guess.
"Air Nomads?" said Bo.
"WRONG! Correct answer is Fire Nation," replied AN.N.
"YOU LOST, HA HA!" shouted Wellard.
Bo stepped backwards slowly and had a panic attack. AN.N congratulated Wellard and let him go.
"The Avatar will come and get me. This needs to stop!" said Bo.
Jing and the others arrived in Bo's room to rescue her. "BO!" shouted Jing. Bo ran towards Jing.
"Jing! WATCH OUT!" shouted Bo.
Bo screamed as she was lasered by AN.N, and Jing and the others were shocked. Jing knelt down on the spot where Bo got lasered. Jing's expression was dark as he turned angry. Jing turned into the Avatar State and managed to destroy AN.N's laser. Team Avatar was arrested, and they were sent to the man who was stalking Jing. His name is Gus.
"Well, well, Avatar Jing, the lost earthbender," said Gus.
"You murderous piece of..." said Jing, though eh was interrupted by Gus.
"Ah, ah, ah, be careful what you say, Avatar, I think it's time I told you about my secret," said Gus.
"What are you talking about?" said Jing.
Gus stared at Jing and said nothing to him.
"Well, we demand answers!" said Jinora.
"Very well, I am a soldier to a very rare colony in the Northern Water Tribe. We are a new bending skill which will make us one..." explained Gus.
Jing replied, "I don't understand?"
"I am an Icebender!" said Gus.
Everyone was freaked out over that revelation. Jing still did not comprehend.
"An Icebender? You mean Waterbender? Icebending does not exist!" said Jing.
"NO! ICEBENDER! AND WE EXIST ALRIGHT, BUT YOU WON'T!" an angered Gus shouted.
Gus used his icebending to attack Jing. Jing avoided the ice, and he ripped the floors and kicked towards Gus. Gus avoided and brought up ice shards and began throwing them at Jing. Luli helped Jing by using a water shield to slow the ice shards down. Jing kicked the ice towards Gus. Gus got hit by the Ice boulder and banged his head against the wall. Jing made a rock prison to make sure Gus did not escape.
"You deserve to die, you twisted..." said Jing.
Yet again Gus interrupted and said, "She's NOT dead!"
Jing stood back and replied, "What?"
"She's alive! All of them!" said Gus.
"Alive!? SHE LIVES!!!" said Jing cheerfully. Jing then turned to Gus and said, "Where is she?"
Gus stood silently and smiled.
"WHERE IS BO!?" shouted Jing.
"She's frozen! Try and find her!" Gus said, taunting Jing.
Jing and others left Gus in his earth prison and tried to find Bo in a frozen prison. They reached the room which was below freezing. They saw people from all nations frozen in an icy cell.
"Poor unfortunate souls," said Asami.
"I know. We need to not only release Bo but get everyone out!" said Jing.
"Jing! Bo's over there!" shouted Chang.
Bo's frozen living body was discovered in one ice cell far from the door. Jing rubbed the ice to look through if it was Bo or not.
"It's her! I don't want to punch the ice, I could hurt Bo," said Jing.
Jing was thinking of an idea. Chang was shivering as his drool turned into hard water.
"Right! Everyone stand back!" said Jing. The others stood back and Jing prepared himself for using his Avatar State to break the ice without hurting Bo. The ice fell off, and Bo was shivering. Jinora gave Bo her Air Nomad cape to keep her warm. Jing broke other ice of other people, like Tracy and Zuli. People were cheering and escaping.
"Thank you, Avatar Jing, for saving us, and now I must go," said Anna.
"Bye, Anna," said everyone.
"Avatar Jing?" said a mysterious woman walking towards him.
"Yes, who are you?" said Jing.
She replied, "I knew your past life really well."
Jing looked within him and looked at his vision of Korra's time. The mysterious woman's name was Ting-Ting. Ting-Ting was weeping as she hurt herself after playing a ball game, Korra cheered her up by giving her a plaster. Jing now understood.
"Are you...?" said Jing.
"Yes, I am Ting-Ting, I am Korra's daughter," replied Ting-Ting.
Team Avatar, including Ting-Ting, returned to Republic City with the electricity back on. They were outside the Town Hall to see President Ru. They all got off Lu-Pan.
"I must go back to the Western Air Temple with my siblings. Good luck, Jing, I'll next see you when you learn air," said Jinora.
Jinora left with her glider and headed off.
"Bye, Jinora!" said Jing.
"Avatar, are you coming?" said Asami.
They all entered to find President Ru, and he was sitting there having a smoke and a pint of vodka.
"Oh! Avatar Jing, YOU'RE BACK!" said President Ru.
"Yeah, everything is now back to normal! Except there a secret colony called Icebenders," said Jing.
"Hmm, they sound dangerous. But I'm sure there's nothing to worry about," replied President Ru.
"So now what?" said Luli.
"Now, Avatar, you must begin your firebending training!" said President Ru.
"Actually, before I do, I need to find my parents!" said Jing.
"Okay, Avatar, I will call a nationwide search," said President Ru.
Jing was happy that the search for his family was on. Ting-Ting patted him on the back, and Luli and Chang hugged him.
Back in the Icebenders Prison, Gus was in his earth prison, waiting for someone to help him. A door opened and someone was walking towards Gus.
"I'm sorry I failed you, Chief Kang!" said Gus.
Kang smiled and froze Gus instantly.
"You are a failure! I will find the Avatar myself!"
Kang walked away and left Gus to die a frozen death as Kang headed off to find the Avatar and finish him once and for all.
End of Chapter
|v - e - dAvatar: The Lost Earthbender Chapters
|Book One: Visions
|Lost Earthbender Found - Avatar Jing Returns - The Missing Avatars - Psychic - A Day at Avatar Korra Park - The Day of the Avatar - Electricity Lockdown - Gameshow of Horror - Part 1: Enter the Games - Gameshow of Horror - Part 2: The Extreme Laser - The Parent Search - TBD 11 - TBD 12 - TBD 13 - TBD 14 - TBD 15 - TBD 16 - TBD 17 - TBD 18 - TBD 19 - TBD 20 - TBD 21 - TBD 22 - A Twist in Time - The Three Avatars - Part 1: Finding Aang - The Three Avatars - Part 2: Battle of the Century
|Book Two: Fire
|Coming Soon in 2015/2016
|Book Three: Air
|Coming Soon in 2016
|Book Four: Water
|Coming Soon in 2016/2017
See more
For the collective works of the author, go here.


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To be annoyed at my SIL??(289 Posts)
I had a baby last week, by c section. DH and I have had no visitors apart from the midwife and my mum for an hour while still in hospital. DHs mum wants to visit but is 2.5 hours away by public transport. We said not yet, to give me a chance to recover and also get the hang of feeding her. I don't feel comfortable having her travel all that way as I will feel mean asking her to leave after a short while- we are in a flat so there is little space.
DH received a text from his sister saying that we had upset his mum and she shouldn't have to make an appointment to see her granddaughter. She says we are being unreasonable and life is too short to wait another couple of weeks.
I'm tired, emotional, sore and sensitive and just don't want people I'm not normally comfortable around in my space- we don't get on that well. AIBU in just saying no for a little while longer? SIL knows that my mum has visited and said that evidences my willingness to have visits but that I am just being picky about who. That's kind of true- I'm a lot more comfortable with my own mother and even then she's only been for an hour!
I wasn't feeling particularly low but do now- feel I'm being emotionally blackmailed. AIBU??
I was hormonal and emotional too when I had my babies. I loved it when my family visited and hated DH's family anywhere near my DC, especially MIL and SIL, but I put up and shut up for DH sake. However I was no pushover and had to be firm when they tried to go against my wishes (finger in the mouth and kissing on the lips etc). So much easier to tell your own family than ILS. DH was really good though and had a word when SIL visited everyday after she finished work.
You have to try to take a step back and give an inch but don't let them take a mile IYSWIM.
FYI mil is a wonderful woman but I needed my mum more than anything as I knew her focus was me, not baby.
Sil is being interfering but I would be gutted not to see a new grandchild. I'm sure she doesn't want entertaining, she's not even coming to see you so much as the new addition to the family...I would be sad to be denied that - sorry...
I hope, hope that when my son has his babies, I will get to see them as newborns.
YABU I'm afraid. I understand wanting space, but everyone wants to meet a new baby, especially the grandparents.
All the madness of visitors soon dies off and you're left to your own devices, so for the sake of peace within the family just let her visit and enjoy this special time.
But redtractor the MIL isn't actually putting on any pressure, the baby is a week old and the MIL has just asked when she can visit, that isn't pressure it's just totally normal behaviour. Newborn babies are lovely that's why people want to see them.
jammiedonut similar with my IL's. My DM helps loads after both DC's were born. Couldn't manage with her.
DH used to moan that FIL should move near to us and come round whenever he wants, cos DM is round here all the time (cleaning, feeding, nappy changing). So i said that's fine, he can share the chores with DM. (FIL does nothing, apart from make us run around after him and extract money from us) He soon saw my point and no longer goes on about it.
It is very easy on these threads to look at your own experience and project that onto what is happening with the OP. whilst it is useful to get other perspectives no where on this thread has the OP said that her MIL has ranted, demanded to come over or even a whisper of competitive grannying. It has all come about as a result of a text from the SIL (who should be able to say to her brother that their mother is upset - ideally not in a text)
We don't know what the MIL has said or done, it sounds as though she would just like to visit her son, DIL and see the new baby. Only the OP will know how she will be. What is wrong with that? Her son can talk to her and explain how the OP is feeling and agree something that hopefully keeps everyone happy.
If she had shown no interest, how would have felt honestly? This is a new experience for the father too.
If you cared for your MIL you would have found a way to facilitate a short visit from her.
Me too nfk I also just can't imagine stopping DH from introducing his first child to his parents.
Cravey I agree with you, first visitors to hospital were my mum and dad and DMIL, all came together and went out for a meal afterwards. It's like what one of the other posters said, mil is just as much family as your mum no difference. Would your mil not help out a bit when she came to visit. I used to love it when my mum or mil came round as they would look after the baby while I went for a sleep or bath.
MrsLyman not stopping MIL from ever seeing the baby, just delaying by a couple of weeks until she feels a bit better.
'The relationship you have with your Mum is completely different.'
Completely agree with this. From the OP's mums' perspective, her DD has undergone fairly major surgery and it is only natural she wants to be with her child. I think the MIL needs to be a bit more understanding and the SIL is massively out of order - at the very least she should have rang her brother. I can understand why MIL feels like she is being denied access to her grandchild but lets be honest tiny babies are boring as shit to other people. There will be plenty of opportunities when the OP is fully recovered and the baby is a little older when both grannies can bond with their grandchild. So no the OP is NBU.
I know she will meet him eventually, but I know my DH would have been really upset if I'd put his parents off from visiting as he's really close to them.
To be fair it doesn't sound as though mil has done anything wrong really. She's understandably a bit sad at having to wait a bit but hasn't put any pressure on OP. It's the sil who has stuck her snout in!
Tiny babies aren't boring as shit to their grandmothers!
I don't see why she can't stay in bed and recover while dh introduces his baby to his mum. It is a very special moment and it seems a bit extreme to deny it.
Having a child invariably introduces all sorts of new permutations into relationships that may be complicated from the start.
Your MIL is no longer just your MIL, she is also the baby's grandmother and they will (hopefully) go on to have a relationship together that will be all their own and not of your making.
Your relationship with your mother will be closer than with your MIL, but to the baby they are both his grandmothers.
Your dh is no longer just your dh, he is also the baby's father and will also have ideas of what he wants as a father and what he wants for his baby.
And in relationship to his mother, he is no longer just her son but also the father of her grandchild.
And in all this, you are also still feeling vulnerable and fragile.
Complicated, complicated.
The only advice I can give is to be as tactful as possible and get your dh to negotiate as much as he can. If you really don't feel up to it, let him exaggerate your physical state slightly. If there is anything he can do to make a potential visit easier- like taking her out to lunch as suggested by one poster- then let him do it.
Also as a mil my dil was my number one concern. Yes the baby was lovely but she had a hard labour and I wanted to help in terms of housework, cooking cleaning etc. I find the mil hatred horrid if I'm honest. We're not all like dragons. Imagine if the op husband had said no to her mother coming. There would be uproar. This is her grandchild an extension of her son and would imagine she is gutted. I know I would be. Also I agree babies are boring but I will be forever grateful to my dil that she allowed me to watch my grandson being born and spend time with him after birth.
"the MIL has just asked when she can visit, that isn't pressure it's just totally normal behaviour. "
and the OP and her DH have told her. And then she's moaned about it to SIL.
Anyway - I still think the OP's husband should respond telling MIL that she can come for an hour and then leave. If she wants what the OP's mother has, if it's a competition or something, then let her have it to keep the peace. But don't involve the SIL.
Ignore SIL and get DH to ring his mother and work things out. Don't justify having your mother at the hospital-it's perfectly understandable that you would want to see your own mother after scary surgery, and quite different from seeing an in-law. I don't think your wishes and needs should be disregarded here, or put last in the order of priorities. But I think your MIL understandably wants a bit of reassurance, and the chance to come over soon.
YABU. She is your husband's mother and your child's grandmother. I fully understand that you are more comfortable with your mum (believe me, I understand!). But you need to treat all grannies equally (unless there is an abusive personality involved).
A section is a big thing to recover from (again, I know - emergency after 30 hr labour), but you could put up with her for a couple of hours surely?
I do not think that you are being unreasonable. You have just had a major op. you have a new baby and your hormones will be all over the place and you are probably exhausted as well.
I don't get what is so wrong with just wanting some peace and quiet until you feel ready for visitors. So what if you let your mum visit? fgs, she is your mum and you are bound to feel more comfortable in her company!
I understand that it is more difficult because it is a longer journey for your MIL so you cannot easily ask her to leave after an hour if you are feeling the need to do so, but could you not retire to the bedroom with baby and leave MIL in the care of your DH?
Unfortunately I had my MIL come and stay several times a year, every time I came home with my new baby she would be there. I found it intrusive and awkward and it definitely impacted on our family bonding, so I guess my POV is a bit affected by that.
YANBU. Your DD is not the property of the family.
When I was in your position as a new mother after a traumatic delivery I felt very vunerable and was railroaded into visits from close friends and inlaws that I didn't want. It turned out DD was ill and needed hospital readmission, bf was failing and my mental health took a long time to recover. It was truly the most stressful period of my entire life. I really didn't need people enforcing their 'right' to view my baby, I'm sure you don't either. Focus on establishing your feeding and getting to know your LO.
You are absolutely right to put your own feelings first on this one. Some people really do think you produced a child just so that they can view it at their leisure.
Am I the the only person who wasn't dying a week after birth, I have had an emergency C section, a planned section and a normal birth and even after the emergency although feeling tender I wasn't lying in bed half dead, I was up and about, I know people wont like what I'm saying here but stop being so fucking precious.
Suck it up, invite your MIL, get your DH to buy some cake and make her a cup of tea and be proud when this lady who obviously loves her DS gazes at your wonderful baby, it's such a precious time having a newborn, please don't let your MIL miss out.
I also have to add that if I had tried at any time to dictate to my DH that his parents couldn't see our DCs he would have quite rightly informed me that I was unhinged and a bit controlling.
Could SiL not arrange to drive her Mum down for a few hours then take her ome again later or even pick her up te following day?
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