text:
CORE Names Ryan Fitzpatrick Director of Sales for Chelsea Office
March 05, 2013New York, NY – (March 5, 2013) – CORE is pleased to announce the promotion of Senior Vice President, Ryan Fitzpatrick, to Director of Sales-Chelsea, where he will oversee both the firm's agents and its re-sale business for its Downtown office. Ryan sales successes have has surpassed $390Million in total sales volume (including units currently under contract and resales) since 2006.
CORE CEO Shaun Osher says, "Having worked as a successful agent for seven years, Ryan's hands on experience has given him a deep understanding of the day-to-day challenges faced by our agents and clients." Osher adds, "Ryan's stellar reputation for professionalism and high level of integrity precedes him. His understanding of our culture, brand, and business model at CORE made him a natural choice for this position. I am looking forward to working alongside him."
Fitzpatrick is excited about the opportunity saying, "This is an exciting time for our industry as the market is so strong. I look forward to building on CORE's incredible resources, specifically its roster of talented agents known in the industry for their knowledge, passion and professionalism,." Fitzpatrick adds, "This position is a unique opportunity for me in that it marries my experience in real estate sales with my prior tenure in the corporate world overseeing a team in financial services. Working with the strong group of agents, as well as Shaun Osher and the leadership team, I feel we can do something really special."
Most recently, Ryan has been part of the CORE team overseeing sales and marketing for the record-breaking Walker Tower. Prior to residential real estate, Ryan enjoyed a successful tenure at Standard & Poor's, where he led analyses of public debt offerings in the housing sector. Ryan also worked in the realm of non-profit community development and advocacy on urban land use issues in New York, drawing on his academic experience at Stanford University where he received his BA. Ryan went on to receive an MBA from Yale University.
CORE's current Director of Sales, Reba Miller, will transition her role to the new Madison Avenue office opening in April. CORE Madison Avenue is a 3,500-square foot location at 673 Madison Avenue at 61st Street, set in a prime retail location and occupying the second and third floors of a historic brownstone.
About CORE
CORE is a real estate sales and marketing firm delivering the best in brokerage, communications and advisory services for the luxury residential segment. In addition, CORE's elite group of highly experienced and successful professionals service developers who value efficient, no-nonsense results. CORE was founded by Shaun Osher as a full-service boutique firm with a strict adherence to the principles of integrity, efficiency and results. For more information visit www.corenyc.com.


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
using url tags to transfer information between formsAsked by UoNSU on June 26, 2013 at 05:57 AM
I am trying to set up a form that pulls data from another of my forms and using url parameters to do this.
I amtesting it at the moment to see if it will work on a biggger scale but I am trying to pull the data from this formhttp://form.jotformpro.com/form/31062749171957
into this form http://form.jotform.co/form/31753845232858
This is the code I have
but it doesn't seem to work - can someone help me with what I am doing wrong
did you get an answer to this .....I have the same question
The URL parameters should have the actual field name excluding "_".
Here is the sample url to prepopulate your form: http://form.jotform.co/form/31753845232858?fullName5[first]=firstname.lastname@example.com
Please refer this guide for more information on this: http://www.jotform.com/help/71-Prepopulating-the-fields-to-your-JotForm-via-URL-parameters
Let us know if you need further assistance.
but how do i make this conditional on the information that the user has submitted? In your example I get only your information into the new form - I want the data typed on the original form to be the data i get in the second form.
I have it working in the example below for the first name field only, but it does not pull the information from other fields
You will need to set the Send Post Data to 'Yes' in Setup & Embed > Preferences > Advanced Settings.
You will to create a PHP page that will forward the data to your other form. Click here for a more detailed instruction on how to send data to another form. You can also check this link for a live sample.
If you need further assistance, please contact us again.
This is exactly what i need.........Is it possible that you can walk me through how to do this. I have jot form 1 and want to dyanmically pass values to prepopulate jot form 2.
I am sorry this is not helpful.......I do not see where to configure or
custom code......I do see several examples that fit my need by not how or
where to do it.
Essentially I want to dynamically pass values from one jot form to another
prepopulating the second to from with values from the first.
does this require custom code if yes is there an example of how to do it.
It is only a few fields that I want to pass.
I will create a sample for you explaining how to prepopulate your form with the value of another form field. I will also explain the steps.
We are very proud to announce that our amazing team of developers have released Jotform Webhooks, this feature would replace Send Post Data + Custom URL Thank You Page workaround in order to help you transfer forms data to your own database using a script code
Please find out more about it here

subset: commoncrawl


text:
The 17-storey hotel has a total of 198 rooms in a variety of fashionable suites, executive rooms and business rooms in a warm atmosphere. Also on offer are four specially designed private dining rooms, respectively named 'good fortune', 'ample salary', 'longevity' and 'happy life'. The air-conditioned establishment welcomes guests in a lobby with 24-hour reception and check-out services. Lift access, a breakfast room and conference facilities are provided.
The hotel is located in the city centre, 20 km from the airport and 10 km from the railway station.
The hotel provides the usual modern comforts in luxuriously appointed guest rooms and suites, all with broadband Internet access. The en suite bathrooms are fitted with a shower. A double bed, direct dial telephone, satellite/cable TV, air conditioning and central heating feature as standard.
The hotel offers a breakfast buffet.
American Express, Diners Club, JCB, MasterCard and VISA are accepted as methods of payment.
Room Facilities:
Direct dial telephone
Satellite cable TV
Internet access
Double bed
Central airconditioned
Central heating


text:
Dear GDUI Members and Friends,
Board Meeting Recording: If you missed our board meeting on Saturday, you can listen to the proceedings from beginning to end by using Dropbox or SendSpace or simply dialing your phone. Here's the information you need from Sarah:
Hello GDUI members and friends,
Provided below are three ways to access the recording of the March 18, 2017 GDUI board meeting. The recording is 2 hours and 15 minutes long.
Drop Box:
Send Space:
Play back number: 712.432.1085
Access code: 919245 followed by the # key.
Sarah Calhoun, GDUI Secretary & Office Manager
Town Hall Meeting Recording : Were you meaning to get around to listening to the recording of our February 18 town hall meeting, but life got in the way? Never mind! Although the phone recording of Saturday's board meeting has replaced the town hall meeting recording that's available on the free-conference-calling line, you can still access our Dropbox to listen. Here's the link you need:
PawTracks News! Nolan has resigned as editor of PawTracks. He needs some time to deal with some health issues, and so, reluctantly we accepted his resignation. I don't think I can find adequate words for thanking Nolan for his great work as editor of our magazine. When our administration began, the magazine had essentially disappeared. Issues of PawTracks were being published only sporadically at best and, although the small committee of former board members who had valiantly tried to keep it going were doing there best, its quality had declined. I was so excited when Nolan agreed to take on the job of bringing PawTracks back to life, and I know that all of us have appreciated all of the improvements he made, including bringing back in-person narration! Thank you, Nolan.
Nolan will continue to be involved with our publications as much as he can. He has indicated his willingness to continue narrating the magazine, and he will continue his involvement with the GDUI Juno Report. We are pleased and grateful.
At Saturday's meeting, the GDUI Board unanimously approved the appointment of our new PawTracks editor, Will Burley! Welcome, Will, to your new GDUI role as editor! We are pleased and grateful to you for stepping into this role.
Wasting no time, Will plans an April 2017 Spring issue, so if you have contributions to make to PawTracks – and we hope you do! – please send them to Will by the publication deadline of March 31. Write "PawTracks" in the subject field, and send all PawTracks articles, poems, letters, or other submissions here: email@example.com
Dues Increases: Another action that our board took at Saturday's meeting was to increase GDUI's annual and Life Members' dues. I wish I hadn't had to write that sentence, but GDUI's financial predicament has truly left us no choice. GDUI's dues have not been raised since 2002, when they increased from $12.00 annually to $15.00. At some time during those intervening years, Life Members' dues also increased to $250 for a lifetime of membership. It is unfortunate that none of GDUI's boards were willing to consider raising dues in the intervening fifteen years. If they had, the increase that we are making now might seem a little less dramatic. However, no one raised dues during all that time, and expenses mounted — for everything from the toll-free phone line, to administrative banking fees, to PawTracks production, to voting ($5 for each member!) — over that decade and a half, and we have found ourselves in a situation of having no choice other than to ask you to pay more. We hope and believe that the membership benefits you derive from being part of our organization justify our asking you to pay more. As of September 1, GDUI annual dues will increase to $25 per year, and if you want to become a life member of GDUI, your dues will be $400. We will continue to work out a way for life members to split their payments into timed installments. And, of course, if you would like to become a life member during the next few months, before the increase goes into effect in September, you'll be benefitting from a significant discount. Please contact Sarah for all of the details and to set up your payment plan! Call 866.799.8436, toll free in the USA.
Your donations to GDUI are always welcomed and appreciated, and our fund-raising committee is pursuing several additional strategies for bringing in dollars, including GDUI's participation in the 2017 ACB Convention Brenda Dillon Memorial Walk! We will be sharing the details about our GDUI team, how you and your guide dog can join as real-time or virtual walker(s), and how you can contact friends and family members to make ACB/GDUI donations grow, in a coming announcement.
Remember, the Record Date, by which time you need to be a paid-in-full member of GDUI for 2017, is rapidly approaching.
That date is April 7. All members who have joined by that date are eligible to vote in our May election. Election dates are May 27 through June 4. And, if you want to run for one of the two open board seats, you need to contact the Chairperson of our Membership Committee, Dixie Sanderson, by April 1. Here's the link where you'll find all the details about the coming election: http://guidedogusersinc.org/announcing-gdui-2017-elections/.
During coming weeks, the Nominations Committee will be eager to receive your questions for the two candidates' forums which will take place in late April and early May, and the Bylaws Committee will be meeting to discuss the several amendments to GDUI bylaws that members have proposed. If you have submitted an amendment, expect to hear from Maria Hansen, who chairs our Bylaws and Resolutions Committee, to schedule a time when the committee can consult with you via telephone about your proposed constitutional change.
The First-Timers' Contest: We hope that lots of you are working on your essays to apply to win one of two "First Timers' Awards," for attending our GDUI convention in Sparks, Nevada. The deadline for submitting your application is April 15. If you win, you'll be one of two people to receive $955 when you arrive at the GDUI convention suite. Here's the link where you'll find all of the First-Timers' details:
Speaking of Convention … and Awards …
It's time to think about rewarding those people who have improved our lives as users of guide dogs over the past year or so. Here's the announcement from Charlie Crawford who is chairing our 2017 GDUI Awards Committee:
Hi GDUI members and friends,
It's that time of year again: Help GDUI Honor the People Who Make a Positive Difference in the Lives of Guide Dog Handlers! Guide Dog Users, Inc. has a long tradition of honoring the people who have assisted us as guide dog handlers and improved our communities in positive ways.
Again this year, we are seeking candidates for our Ethel Bender and Moffat-Gleitz awards. In addition, we wish to honor the writers and communicators who make our quarterly magazine, PawTracks, and other productions such as the GDUI Juno Report, such informative and entertaining publications.
We encourage you to think about all the PawTracks and GDUI Juno contributors whose stories, articles, and poems have informed and entertained you in the last year. If someone moved you or made you smile or taught you something new about yourself or your dog or your experience as a person who is blind, we hope you will nominate that person for the GDUI PawTracks Excellence in media Award.
Next, our Ethel Bender award honors a sighted person who has made a significant contribution to the guide dog community. Past winners included Ted Zubrycki, Lukas Frank and Michael Lilly.
Our third award, the Moffat-Gleitz Award honors a person who is blind and who has significantly improved the lives of guide dog users.
Awards will be presented at the GDUI annual luncheon on Wednesday, July 5, in Sparks, Nevada.
Please send us the name(s) of the person(S) an where or how to contact them and for which award they are being nominated and why? Your nomination does not need to be long, but worded sufficiently to give us a clear understanding about why you choose to nominate that person or persons. Please also include your contact information so we can follow up with you if we require more information. Send your nominations to Charlie Crawford at this address:
firstname.lastname@example.com and Put the words: "GDUI AWARD Nomination(s)" in your subject line.
No access to e-mail? Call GDUI's office manager, Sarah Calhoun at 866.799.8436. Please submit your nominations by June 1, 2017, and thank you for helping us honor the people who make life for guide dog teams better every day.
Charlie Crawford: Chair, GDUI Awards Committee.
That wraps up our latest announcements. As always, we appreciate your friendship and support.
Penny Reeder, President
Guide Dog Users, Inc.
Deanna Noriega, First Vice President
Guide Dog Users, Inc.
GDUI Website: http://www.guidedogusersinc.org/
Call us toll-free at 866.799.8436
Like and visit us on Facebook: https://www.facebook.com/GDUInc
Follow us at Twitter: @GDUInc
Enjoy the GDUI Juno Report on ACB Radio Mainstream, at8:00 p.m., EST, every Friday of every month, here: http://www.acbradio.org/mainstream.
Download or subscribe to the GDUI Juno Report pod cast here: http://acbradio.org/gdr.xml or search for the GDUI Juno Report on ITunes.
Support GDUI when you use this link to shop at Amazon.com: http://smile.amazon.com/ch/52-1871119.
To join the GDUI-Announce List, visit this link: http://guidedogusersinc.org/join/.
To subscribe to the GDUI Chat list, visit this link: firstname.lastname@example.com
To subscribe to the (members only) GDUI Business list, visit this link: email@example.com
A recording of each GDUI announcement is available here: 712.432.1281. Enter the Access Code 488062 followed by the number sign. When prompted, enter the Reference Code, No. 1. The recording will remain available until it is replaced by a recording of the next GDUI Announcement. Please share this information with friends who may not have access to the internet. Thank you. We look forward to sharing information with all of our GDUI members and friends.


text:
The vehicle in your driveway is head and shoulders above the rest when it comes to all the vehicles currently being driven today. Your Cadillac dealership isn't the only place to locate genuine or original equipment quality parts to repair your Cimarron. Make no mistake, you bought your Cimarron because you knew that you wanted long-lasting quality and a trusted brand both rolled up into one vehicle. You bought your Cimarron because its performance and American engineering struck a chord inside you; so why would you be okay with second rate when it comes to the highest quality auto parts?
In order to get full functionality out of your car, you have to be able to keep your chassis sheet metal from making contact with other body parts. The door weatherstrips on your Cadillac Cimarron seal the gap between the door shells and the chassis unibody; ensuring that you and your passengers can keep the outside world separate while driving down the road. Made of EPDM rubber material for maximum flexibility and durability, the door weatherstrip seals are an absolutely vital component to every vehicle on the road. When your Cadillac Cimarron door seals have begun to deteriorate, your vehicle would be subject to rust caused by exposure to the elements and constant dents and dings from metal to metal contact.
Bring your Cimarron back to life with the best new or rebuilt parts from the experts at Car Parts Discount. Don't let a Door Weatherstrip Seal repair spell the end of your car, truck, or SUV, and don't risk lowering its resale value by installing anything but the highest quality repair parts. Given all the trouble that can be involved with repairing damage to your car or truck, let www.carpartsdiscount.com be the number one store you go to make purchasing it easy. Need 1988, 1987, 1986, 1985, 1984, 1983, 1982 Cadillac Cimarron Door Weatherstrip Seal parts? We've got them right here.


text:
All Android applications categories
Chinese Health with Qi Gong.
Qi Gong is an ancient Chinese health practice that coordinates breathing patterns with movement to maintain and improve health and well-being.
Master Tan Fung Ya have been practicing the ancient art of QiGong for over fifty years. He has developed this video to help students both old and young, sedentary and active achieve greater health and well being.
This video is in two parts: basic and intermediate.
Basic Chinese Exercise - QiGong
The discipline of basic QiGong is comprised of eight elements. These elements performed daily will improve circulation, build a stronger immune system and enhance mental sharpness.
Element 1 - Improving Digestion and Spine
Element 2 - Invigorating Nervous System
Element 3 - Balance of Energy
Element 4 - Flexibility of Lower Body
Element 5 - Lung, Intestine, Reproductive Organs
Element 6 - Kidneys and renal-system pressure points
Element 7 - Alleviating Headache, Insomnia & Fatigue
Element 8 - Sharpening Mental Capacity and Focus
Intermediate QiGong is for those who wish to further build strength, stamina and grace.
Intermediate Che Gong is comprised of eight elements and should be performed as frequently as desired.
Element 1 - Lungs, heart and stomach
Element 2 - Pelvis Balance
Element 3 - Aiding Digestion
Element 4 - Preventing from Five Hardships
Element 5 - Diminishing Occurrence of Heartburns
Element 6 - Bladder, Kidney and Liver Function
Element 7 - Building Strength
Element 8 - Coordinating Kidney, Bladder and Back
Tags: qi exercise free and , qigong , qigong kidney exercise images , qi gong word español , qi gong , video qi gong gratuite , kidney exercise , heal yourself with qigong , the eight fine qigong exercises
from 5 reviews
Download Easy Chinese Exercise Qi Gong
Free - V2.0 - 94M
Sorry ...
This app is no longer available.
Share this app


text:
This property is no longer available.Search for Available Property.
3.85 acres in Crow Wing County, Minnesota
Build your dream home on a beautiful 3.85 acre lot. Enjoy the privacy of a serene setting with mature trees in Pine River. This property offers a great location super close to the White Fish Chain, golfing and so much more.
From Pine River go east on County Rd 1 to south on Long Farm Rd to Woodcrest Lane. Lot #11 on sw corner of Long Farm Rd and Woodcrest Lane.
Tax Data (Show Tax Data)
Loading Tax Data
Other Land for Sale in Crow Wing County
- Properties for Sale in Nearby Counties
- Aitkin County Land for Sale
- Cass County Land for Sale
- Mille Lacs County Land for Sale
- Morrison County Land for Sale
NOTE: The information contained herein is provided as general information only and has been obtained from sources deemed reliable. It is provided without any guaranty, warranty or representation, expressed or implied, made by Lands of America, or any related entity, as to the accuracy or completeness of the information. The information is presented subject to errors, omissions, change of price or conditions, prior sale or withdrawal without notice. Prospective purchasers should make their own investigations, projections and conclusions concerning the information.


text:
Get set for summer.... More..
Life is the bubbles . More..
Bring the outdoors in this May. More..
More over eggs, we've found more fab ways to eat chocolate this Easter…. More..
We're hopping with egg-citment over these gorgeous bunny-themed gifts…
. More..
Inspire your little explorers this spring…
. More..
Update their room with animal inspired pieces…. More..
Finding the perfect Christmas present for children is never easy, so we thought we'd ask the experts (the kids) what they would they really like for Christmas.... More..
The elves have been working hard in Santa's workshop to help young imaginations soar with Lidl Northern Ireland's range of interactive toys encouraging creativity and learning at playtime.. More..
Festive finds for a magical celebration. More..
From ghastly gifts to looks to thrill, make your supernatural party go with a bang this All Hallows' Eve…
. More..
We dare you to get your fangs into these come October 31. More..
The woods are lovely, dark and deep, and here the little creatures sleep…
. More..
Why are these gorgeous gifts like a birthday cake? They are just so sweet…. More..
These stylish essentials will get the new term off to a great start
. More..
Back to top
Web design and development by Creative Online Media, Belfast. Copyright 2007-2008. All rights reserved.
This page is valid XHTML 1.0 Strict, CSS


text:
Pet Sitter in New Portland
Hello my name is Abbie Bruni and I have been working with dogs and animals for a long time! I am a true animal lover and I would like to let you know that I am available to dog and pet sit, walk and play!! I have my own transportation, lots of experience, references and I am comfortable pet sitting at your home. I also have a very flexible schedule and can be available on Holidays.
Pet Sitter Details
|Email Address
|Phone Number
|207-289-... Confirmed
Dog Walking
Pet Feeding
Pet Sitting
Pet Grooming
Overnight Care
Notes made here will only be seen by you. If you want to message the owner of this listing
More in New Portland, Maine
Looking for Pet Sitter jobs in New Portland, ME?
Find available jobs in your city.
Featured Pet Care Providers near New Portland, ME


text:
Welcome to my latest Work In Progress, this week sees the beginning of my old 1/35 scale Italeri A1-1W USMC Cobra attack helicopter.
I bought this kit years ago and recently found it when going through my kit collection. It's been ages since I've built a helicopter and I really want to have a crack at this one!
The kit is a re-issue of the 1992 MRC moulding and it has stood up well to the passage of time. The decals were not so great so I am replacing them with an after market option from Two Bobs Aviation Graphics.
The kit does not have the NTS upgrade so I will be making a few modifications here and there, but I will keeping this build as hassle free as I can as I want to enjoy building it as opposed to worrying about accuracy.
I plan to end up with a Desert Storm machine something like this:
I have started with the cockpit, no aftermarket parts here, this is all hand painted by me!
Next came the pilot's instrument panel. There is a lot of raised detail here so it's worth careful painting to bring the panel to life.
The gunner's panel. Again this piece benefits from careful painting.
I want to display my Cobra with her crew so I found a USMC Cobra pilot from D-Toys. It's a fantastic moulding, but expensive! Plus I need two figures!
I solved my problem by modifying a 1960's Huey pilot, I have added modern body armour, patch pockets and NVG gear.


text:
- About
- For researchers
- For organizations
- Contact us
- Log in or Sign up
Data from: Rapid evolution of cheating mitochondrial genomes in small yeast populations
Date Published: July 31, 2013
DOI: http://dx.doi.org/10.5061/dryad.2018h
Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data.
|Counts of petite and grande colonies for spontaneous and evolved petites.
|15 times
|Three spreadsheets are included: "spontaneous" gives the counts of petite and grande colonies for spontaneous petite mutants; "spont.posteriors" gives the MCMC estimates for the spontaneous mutants; "evolved" gives the counts of petite and grande colonies for the three ancestors (X, Y, and Z) selected at each of five effective population sizes (1, 3, 10, 32, 100). The mip1-deleted control is also included on the "evolved" and "spontaneous" spreadsheets.
|Jasmin_Zeyl_Evolution_petites_data.xlsx (64.73 Kb)
|View File Details
When using this data, please cite the original publication:
Additionally, please cite the Dryad data package:
We encourage organizations to:


text:
Branham Accessories - BAK Tonneau Covers in Cullman, AL
Branham Accessories
101-A Arnold St Nw
Cullman, AL 35055
Cullman County Alabama
Phone: 256-775-1719
Website: Not Yet Verified
Store Location
As an authorized BAK tonneau cover and accessories dealer, Branham Accessories located in Cullman, AL, is committed to helping you make the best tonneau cover decision for your pick-up truck and your lifestyle. Branham Accessories has been in business for many years and has a wealth of experience when it comes to all sorts of truck bed covers and accessories. Branham Accessories are expert tonneau cover installers and will ensure that your truck bed cover is installed professionally and properly. Simply put, Branham Accessories offers the best choice of soft, roll-up, hard folding, retractable, and custom fit truck bed covers and accessories. They also sell and install most pickup truck accessories. If you want the best deal on a brand new BAK tonneau cover and want it done right, quickly and with no remorse, then head over to Branham Accessories in Cullman your local BAK Industries dealer, needless to say, all of their work is guaranteed!
BAK Industries Videos
- Dealer
- New
Customer Reviews
Total Reviews (0)
Write the First Review!
You must login to post a review.


text:
This wiki is dedicated to sharing information about Bay Area faves: hiking trails, restaurants, jazz clubs, mechanics, salsa lessons and more. It is maintained by members of the Soup community http://ladle.com and friends
Best Activities Lindy lessons in GG park and Union Square. (http://www.lindylist.com) Once-a-month Sea Chanteys on Hyde Pier (http://tallships.tribe.net) Free docent led tours of the botanical gardens in Golden Gate Park (http://www.sfbotanicalgarden.org/visiting/page3.html) Free movies at the MOMA (When there is a movie playing at the MOMA theater, anyone can walk in for free as it is on the first floor (along with the cafe and gift shop) and you only need a ticket to go to the upper floors. I think it is either 2PM or 3 PM daily when they are screening. Art Gallery Open Houses: In San Francisco, this is held the first Thursday of the month and includes all galleries who are members of the San Francisco Art Dealers Association (http://www.sfada.com). In Oakland, the first Friday of the month is Artmurmr (http://www.oaklandartmurmur.com)
Best Restaurants
Best Hikes Purisima
Here's a compendium of all the days the various museums in the Bay Area are free... For the most part remember FIRST WEEK OF EVERY MONTH!!
( http://www.booboolina.com <http://www.booboolina.com/culture/>
Museum of Craft and Folk Art 51 Yerba Buena Lane First Tuesday of every month free.
Yerba Buena Center for the Arts 701 Mission St. at 3rd Galleries are free the first Tuesday of every month.
San Francisco Museum of Modern Art (closes 545 ) 151 Third St. between Mission and Howard First Tuesday of each month free.
The de Young Museum 930-530 50 Hagiwara Tea Garden Drive First Tuesday of each month free.
Legion of Honor 930-5 Lincoln Park in the Presidio First Tuesday of each month free.
Conservatory of Flowers John F. Kennedy Dr. in Golden Gate First Tuesday of each month free.
Asian Art Museum 200 Larkin St. between Fulton and McAllister First Tuesday of Every month free.
California Academy of Sciences 875 Howard Street First Wednesday of every month free.
Cartoon Art Museum 655 Mission St. The first Tuesday of every calendar month is "Pay What You Wish Day."
First Wednesday – California Academy of Sciences, Exploratorium, Mexican Museum, Museo ItaloAmericano, San Francisco African American Museum and San Francisco > Zoo.
Museo ItaloAmericano Fort Mason Center Building C Free the first Wednesday of every month.
Exploratorium hours 10-5 3601 Lyon St. First Wednesday of each month free. Groups of 10 or more require reservations.
San Francisco Zoo open 10-5 Sloat Blvd. at 47th Ave. First Wednesday of every month free.
First Thursday - Chinese Historical Society of America, Pez Museum (Burlingame) and the San > Francisco Museum of Craft & Design.
The Chinese Historical Society of America 965 Clay Street First Thursday of the month is free.
The Contemporary Jewish Museum 121 Steuart Street between Mission and Howard Third Monday of each month free.
Second Saturdays: Bay Area Discovery Museum (Sausalito). Second Sundays: Oakland Museum of California.
Third Mondays: Contemporary Jewish Museum.
All Thursdays: Hearst Museum of Anthropology (Berkeley).
Free public tours: San Francisco City Hall, Contemporary Jewish Museum (12:30 p.m. Sun. and Wed.), Gold Rush Trail, San Francisco City Guides, San Francisco Botanical Garden, others.
Always free: African-American Museum and Library (Oakland), Cable Car Museum, CCA Wattis Institute for Contemporary Arts, Chinese Cultural Center, Maritime Museum, Mills College Art Museum (Oakland), Museum of Children's Art Oakland, Museum of the City of San Francisco, Octagon House, Presidio Museum, Randall Museum, San Francisco Center for the Book, San Francisco Fire Museum, Takara Sake USA Museum (Berkeley), Triton Museum (Santa Clara).
Sometimes free: Habitot, including Oct. 6 (Mooncake Festival) and Oct. 8 (Indigenous People's Day).
Free, but coin-operated: Musée Mécanique.


text:
A bowling alley is a building with many long, narrow tracks that are used in bowling games. Bowling alleys are more or less undesirable as a base, because they offer little more than a place to bowl a game or two with a couple of buddies or some scraps in the snack bar.
- Bowling alleys usually have no windows, making them easier to secure.
- Bowling balls and pins can be used as makeshift weapons if nothing else is available.
- A little bit of food in the snack bar may still be salvageable.
- Not many people will think to raid a bowling alley because they don't have many resources or equipment that can be used
- Arcade machines that are found in some bowling alleys could be used as barricades.
- Bowling shoes of every size will be in abundance.
- The lanes may require power to remain functional.
- A bowling alley in its normal use is pretty noisy.
- Not many resources can be found in a bowling alley.
- The food will probably be taken or spoiled by the time a group of survivors stumbles to it because it most likely isn't canned or otherwise preserved.
- Any type of food that would be found in the snack bar is pretty unhealthy.


text:
The hotel offers 47 rooms in total and guests are welcomed in a lobby with 24-hour reception and check-out services, a hotel safe, currency exchange facilities, a cloakroom, newspaper stand and lift access to the upper floors. There is a caf�/bar, a restaurant, a terrace, and a 2nd floor balcony offering city views. Guests may also take advantage of the conference facilities, wireless Internet access, room and laundry services, and a car park.
This charming city hotel is located in a quiet residential area in the city centre, just 400 metres from the heart of the city's Austurv�llur Parliament Square and other city landmarks and attractions, restaurants and caf�s, museums, theatres, art galleries, shopping areas and nightlife spots. Guests will find links to the public transport network 3 minutes away on foot. Laugardalur Park is approximately 4 km from the business hotel, the beach in Nautolsvik is around a 30-minute walk, and the Blue Mountains ski area is roughly 30 minutes by car.
Each room features an en suite bathroom with shower and hairdryer, a double bed, TV, Internet access, individually regulated heating, and a private terrace. Other in-room amenities include a direct dial telephone, radio, hi-fi, safe, minibar, ironing set, and tea and coffee making facilities.
Grafarholt golf course is approximately 11 km from the hotel. Sun loungers can be hired for a fee on the sandy/rocky beach.
The Braudb�r Restaurant specialises in fish, steak and traditional Icelandic dishes. A continental breakfast buffet and a lunch buffet are offered. Set menu and � la carte options are available for lunch and dinner.
American Express, Diners Club, JCB, MasterCard and VISA are accepted as methods of payment.
Room Facilities:
Direct dial telephone
HiFi stereo system
Internet access
Mini fridge
Tea Coffee
Iron and board
Double bed
Central heating
Individual heating


text:
Opera Complaint Against Microsoft Backed by FSFE
Opera Software has filed a complaint against Microsoft's practice of tying Internet Explorer to Windows. The Free Software Foundation Europe (FSFE) has offered its backing of Opera's complaint.
The European Commission Directorate General for Competition followed up just a few days ago on Opera's concern by issuing yet another statement of objections to Microsoft's practice. The FSFE welcomes the initiative and offers the commission assistance in their investigation.
"Web browsers are becoming a critical platform for home and business computing," according to Shane Coughlan, legal coordinator at the FSFE. "The market previously failed to prevent unfair distortion of the desktop environment and we cannot allow such practices to be repeated."
A letter from FSFS president Georg Greve to European competition commissioner Neelie Kroes clearly states, "Although Opera Software does not produce Free Software,... we largely share their assessment and concerns regarding the present situation in the Internet browser market." He goes on to say, "Precisely because [products such as Firefox and Konquerer] abide by industry recognized Open Standards and cannot implement the Microsoft undisclosed and non-compliant 'dialects' of these standards, they often appear 'impaired' when compared with Microsoft's Internet Explorer, which establishes itself as the closed, de facto standard due to [its] dominant position."
Issue 201/2017
Buy this issue as a PDF
Kolab Now Integrates Collabora Online
Kolab Now subscribers now have access to Collabora Online.
Endless OS, a Distribution Without Internet
A new version of Endless OS has been released.
GitHub Announces Open Source Friday Program
They encourage people to take time out and work on open source projects on Friday.
System76 Announces Their Own Distro, Pop!_OS
Pop!_OS is based on Ubuntu and uses the Gnome stack.
Linus Torvalds Talks About His Motivation
He never tires of working on the Linux kernel.
Debian 9 Stretches Its Wings
The new release of Debian has a very strong focus on security.
Trojan Turns Raspberry Pi into a Cryptocurrency Mining Device
Two trojans in the wild are targeting Linux machines.
Fedora 26 Beta Comes with New Features
The workstation comes with the latest Gnome desktop environment and support for many application build systems.
Raspberry Pi Foundation Merges with CoderDojo Foundation
The two foundations join forces to expand their efforts to reach young people.
Samba Security Hole Patched but Risk is Bigger
Millions of devices that use Samba Server are vulnerable to attacks.


text:
Dr. Sara Jane Hendren MD
- Emergency Medicine Aurora, IL
Patient Reviews
Reviews reflect patients' level of satisfaction with the care they received from this provider. Dr. Sara Jane Hendren xhas an overall rating of 3.7.
Dr. Sara Jane Hendren, MD is a Doctor primarily located in Aurora, IL, with another office in Louisville, KY. She has 9 years of experience. Her specialties include Emergency Medicine. Dr. Hendren is affiliated with Rush-Copley Medical Center. She speaks English.
Find out the languages this doctor/ or staff may speak.
View all
Learn where this doctor attended medical school.
View all
Specialties & Expertise
Dr. Sara Jane Hendren has the following 3 areas of expertise, 1 specialty and 1 board certification.
Emergency Medicine
An emergency physician is a doctor who is an expert in handling conditions of an urgent and extremely dangerous nature. These specialists work in the emergency room (ER) departments of hospitals where they oversee cases involving cardiac distress, trauma, fractures, lacerations and other acute conditions.
Emergency physicians are specially trained to make urgent life-saving decisions to treat patients during an emergency medical crisis. These doctors diagnose and stabilize patients before they are either well enough to be discharged, or transferred to the appropriate department for long-term care.
See areas of expertise for treating specific conditions, procedures and surgeries for this doctor.
View all
See the board certifications this doctor has received. Board certifications provide confidence that this doctor meets the nationally recognized standards for education, knowledge and experience.
View all
Health Guides
Get the facts about chronic obstructive pulmonary disease (COPD), including symptoms and complications.
more health guides
Locations & Directions
Nearby Doctors
Dr. Sara Jane Hendren, MD is similar to these 3 Doctors near Aurora, IL.


text:
Hiring a Front End Developer in Arizona
November 18th, 2015
This position is for a Java Engineer role focused on supporting a rapidly growing digital services platform for our Fortune 500 client in Phoenix, AZ. Our client is expanding on online payment services in many different markets and forming new partnerships by building new money movement capabilities in new markets as well as improving existing offerings in current markets. We are looking for a experienced Java J2EE engineer with good industry exposure and passion to excel.
Job Responsibilities
- Work with product manager, project manager, business analysts, architects and engineers to design and build efficient, scalable and reusable enterprise software
- Estimate engineering efforts, attend daily scrums, ensure functional and non-functional deliverables are on schedule, code, perform code review, plan implementations and help roll out system changes to production
- Ensure 100% compliance to technical, architectural, regulatory and strategic standards, policies, and procedures
- Strong quality and security focus including automation, design reviews and unit testing
- Communicate and coordinate with cross-functional business and technology teams
- Mentor developers in coding best practices and system performance fine tuning
- Support rollouts, analyze technical issues and be available for on-call duties to lead resolution
Desired Skills and Experience
- Responsibilities include:
- Serving as a core member of an engineering team that designs and develops software applications
- Writing code and unit tests, working on API specs, automation, and conducting code reviews and testing
- Identifying exciting opportunities for adopting new technologies to solve existing needs and predicting future challenges
- Performing ongoing refactoring of code, utilizing visualization and other techniques to fast-track concepts, and delivering continuous improvement
- Working with product managers to prioritize features for ongoing sprints and managing a list of technical requirements based on industry trends, new technologies, known defects, and issues
- 6+ years software development experience
- Prefer a BS or MS degree in computer science, computer engineering, or other technical discipline
- Demonstrated experience in Agile development, application design, software development, and testing
- Thorough understanding of XML, JSON, Web Service technologies, and data structure fundamentals
- Experience with JavaScript Libraries (such as jQuery, GWT, Dojo, AngularJS), Social Media (Twitter, Facebook, Google+) API's, Search Engine Optimization, Web Page Optimization strategies desired
- Experienced in Node.JS is MUST
- Publishing industry experienced a plus
- Experience with Java applications design and development in an Enterprise setting using J2EE, Spring, SQL DBMS's desired
- Experience with adaptive and responsive techniques
- Expertise in Objected Oriented Analysis and Design across a variety of platforms
- Proven hunger to learn new technologies and translate them into working software
- Ability to effectively communicate to internal and external business partners on solution design
Please send resume to firstname.lastname@example.com
Categories: jobs, Miscellaneous, Parallel HR Solutions is hiring


text:
The content of this page have been removed because it is in violation of Wikia's Terms of Use, specifically, the first two points:
User Conduct
You agree not to use the Service to:
- Abuse, harass, threaten, impersonate or intimidate other Wikia users;
- Post or transmit any content that is obscene, pornographic, abusive, offensive, profane, or otherwise violates any law or right of any third party, or content that contains homophobia, ethnic slurs, religious intolerance, or encourages criminal conduct;
See more: http://www.wikia.com/Terms_of_Use
The purpose of this site is for those on the academic job market to share information about the progress of various searches. It is also a place where information and critiques may be shared about the *general* practices of universities and departments. However, it is *not* a staging area for launching personal attacks on identifiable individuals. The remarks removed from this page (and on Talk:Spanish & Portuguese 2012-2013, which have also been removed) cross the line into "abusive" content (including the use of what might be considered "ethnic slurs"). Future abusive, harrassing or threatening remarks will be deleted and users may be blocked. Thanks. Una74 (talk) 00:29, November 19, 2012 (UTC)


text:
Folks, please slap your eyeballs together for an entertaining interview with the talented Kathryn Meyer Griffith. I have enjoyed a few books of hers so far (The Nameless One & Dinosaur Lake) and have a few more queued up. Today we chat about writing in the horror genre for decades, family, cover art, and much more! Enjoy!
(1) What now-dead author would you like to interview? What are some of the things you would chat about?
I'd like to interview Edgar Allan Poe. He had such a tragic, short life and to this day the real reason for his death is a mystery in itself. Was it drink, drugs or suicide? I think of him as one of the early horror/mystery genre writers. I'd love to talk to him and know what his life had really been like. If he was as unhappy as history sometimes portrays him and why he decided to write the macabre and mysterious. Also at twenty-six he married his thirteen year old cousin. Wouldn't the social networks and media have a field day with that these days?
(2) Reality in my fiction: how important is it? Lengthy travel, cussing, and bathroom breaks happen in real life. How do you address these mundane occurrences in your writings?
I usually don't or I breeze over them as a quick mention. Travel is easy to express; you only hit the highlights. People know, as humans, we have physical needs so why dwell on them in a novel unless they're really necessary to the story or plot; most times they aren't. Also, in my early writing days in the 1980s I bent to the times and my publishers' pushy editors and sometimes put cuss words in my books. I was up against Stephen King, Koontz and others and they cursed in their novels, so I thought I had to also. Though I rarely used curse words myself in my real life. But as the years and books went by I stopped using the heavy curse words or profanity, because I wanted my novels to be read by people of all ages, and now days I hardly use more than a damn or a hell once and a while. I let a character's actions depict their frustration or anger, not profanity.
(3) Which ancient or historical works have you not read and periodically kick yourself for not having made time for them yet?
Ha, ha…a lot of them. I'm afraid I'm one of these authors that like to read what's current in fiction or my genres and not the real old stuff. I did read some of the old classics in high school as most kids did, but since I've been too busy writing to read anything I don't really want to read…and the ancient works are some of what I don't feel like reading. This is a secret I've kept for decades, but now I'm too old to care what others think of me. Writing good stories are all I care about. I was born a storyteller so all I feel I have to do is tell my stories.
(4) If you couldn't be a writer, what would you chose to do?
In my life I've been, and am, an artist and when I was very young I sang out with my brother, Jim. Folk duo in the sixties and then in a few classic rock bands. Then I got married (way too young), got pregnant and dropped out for a while. Started writing out of despair and loneliness. My brother went on to sing out professionally, along with the real day job, as I had, for over thirty years and I went deeper into my art – I was a graphic artist for twenty-three years in the corporate world – and from age twenty-one on into my writing. I really loved singing but I wasn't good enough (my brother always said) to do it for a living. Good thing. I wouldn't have twenty-three novels, two novellas, twelve short stories published and eighteen audio books (soon to be twenty) if I would have continued on with the singing or the art career. But I had a passion for singing and miss it to this day. And my brother? He was a gifted musician and songwriter for over forty years but he's been battling esophageal cancer the last two and can no longer sing or play. He's such a brave man, but he misses the music, too. BUT if I couldn't be any of those three, heck, I'd probably be broke and homeless because I'm a storyteller and that's all I can be/want to be.
(5) What does your Writer's Den look like? Neat and tidy or creative mess? Can you write anywhere or do you need to be holed up in your author cave?
I'm a neat freak. Always have been. Everything has a place in my house and it stays there. I hate messes. I got that from my mother. She always said, "We might be poor but at least we can be neat and clean." And that went for our home as well. And I'm the same way with my home now. I'm very organized and I don't abide clutter. I like to write on my laptop in my living room, TV on for company and a fire in the fireplace, sitting on my plush sofa with a hot cup of my chocolate coffee on the side table. I used to write at a stationary computer in a writing room, but it felt too much like work, so these days I like the comforts of my front room. If I had a deck with a view (trees not neighbors) I'd write out there on nice days, yet I don't have a deck. Darn. I also like to be totally alone when I write. It's easier to go into my make-believe worlds and live what I'm writing.
(6) If you could sit down and have tea (or a beer) with 5 fictional characters, who would you invite to the table?
Beats me. Are you talking about five of MY characters or someone else's? I can't right now off the top of my head pick five other author's characters (except maybe the five main characters in Stephen King's masterpiece end-of-the-world novel The Stand…which would be neat) but if I had to pick five of MY characters I would pick the main five from my apocalyptic end-of-days saga A Time of Demons. One's an angel, Manasseh; one's a demon, Rayner; main character Cassandra and her cohorts, her singer-songwriter brother, Johnny; a carnival clown named Walter; his psychic girlfriend Sarah; and another demon-fighter named Obadiah – all warriors for God fighting demons in the end days as the Rapture approaches. Now I'd LOVE to go on that journey with them in their RV all across the country and fight the good fight with the swords given them by the angels. And I'd have my homemade chocolate coffee with them and maybe bake them a chocolate cake, too. They fight hard so they could use the treats.
(7) Care to share an awkward fangirl/fanboy moment, either one where someone was gushing over your work…..or one where you were gushing over another author's work?
Since I've been writing now for over forty-three years and published since 1984, I do get people who say: "I read your 1993 book Witches when I was in high school, ages ago, and have never forgotten it." Or someone will email me and gush over my very first horror novel published in 1984, Evil Stalks the Night, and how much they loved it…as a kid. Makes me feel so old but it's also flattering that they remember one of my books decades later. I love it.
(8) Cover art can be so important for a book, making or breaking sales. What cover art has caught your eye, that you found stood above other books?
I'm going to be absolutely truthful…there are lots of great covers out there, too many to list, but my favorite are the covers Dawne Dominique has done for me the last five years. They're amazing, especially my Dinosaur Lake covers. And yes I also believe a cover, and a good blurb, is a necessity for selling books, particularly self-published books. I've had so many awful covers when I had no choice in the matter with my legacy publishers, but now I get to pick and design my own (because I started self-publishing in 2012) and Dawne seems to read my mind she's so good. To me a great cover not only conveys the essence of a novel but has dynamic colors and a haunting ambiance. It's got to jump out at you, bite you and then haunt you after it's gone.
(9) What do you do when you are not writing?
Lately (since 2010) I've been so busy bringing out my old novels, rewriting, writing new stories and working with narrator/producers creating the audio books that I have had little spare time for anything else. I hope to be able to slow down when all my books (fifteen are still with a publisher until 2015-2017) are finally self-published and I completely own their rights and have them all out again. But I try to spend time with my husband, of thirty-six years, and my family. I have five siblings and I try to spend as much time with them as I can, especially Jim. I love TV. Dramas and PBS or BBC America mysteries. Game of Thrones type stuff. Star Trek. I like to go to the movies. I love to read, of course. Horror and murder mysteries or anything spooky. I like to bake, sugar cookies and chocolate chip being my favorites, and I enjoy walking in the woods and marveling at nature. Colorful sunsets, ocean or lake vistas can mesmerize me. They give me a sense of peace that as I've gotten older soothes me.
(10) What is the first book you remember reading on your own?
I remember, way back in the dark ages of the mid 1950s, reading the Dick and Jane Primer books and having such a feeling of accomplishment being able to decipher them. I was very young and I had no trouble reading the words. I was so good at it as well as spelling. I loved reading from then on. I began reading everything I could get my hands on, going to the library for free books because my family was so poor, and developed a great love and respect for the written word and authors. But one of the earliest recreational books I ever read that left a real impression on me was a novel called Smokey. I've looked it up recently but it no longer exists and what a pity. It was about this horse and all the travails of its life. So many sad things happened to the horse but in the end the animal came home and grew old happily. It was so poignant it made me cry. I never forgot that book and years later it was one of the reasons I became an author myself. Imagine – being able to make people feel something that much that they cried, or laughed or grew angry. Now that was power. When I was young I thought authors were gods. Now I know they aren't, they're just people – storytellers – like me.
Thanks for having me here, Susan and Dab of Darkness.
And thank you Kathryn!
A short author bio:
Kathryn Meyer Griffith has been an artist and worked as a graphic designer in the corporate world and for newspapers for twenty-three years before she quit to write full time. But she'd already begun writing novels at twenty-one, over forty-four years ago now, and has had twenty-two (10 romantic horror, 2 horror novels, 2 romantic SF horror, 1 romantic suspense, 1 romantic time travel, 1 historical romance, 2 thrillers, and 3 murder mysteries) novels, two novellas and twelve short stories published from Zebra Books, Leisure Books, Avalon Books, The Wild Rose Press, Damnation Books/Eternal Press; she's self-published her last 7 novels with Amazon Kindle Direct. Her Dinosaur Lake novels and Spookie Town Mysteries are her best-sellers. She's been married to Russell for thirty-six years; has a son and two grandchildren. She has one cat, Sasha, and the three of them live happily in an old house in the heart of a small quaint town in Illinois. Though she's been an artist, and a singer in her youth with her brother Jim, writing has always been her greatest passion, her butterfly stage, and she'll probably write stories until the day she dies…or until her memory goes. 2012 & 2014 EPIC EBOOK AWARDS *FINALIST* for The Last Vampire–Revised Author's Edition and Dinosaur Lake.
Places to Find Kathryn & Her Books
All Kathryn Meyer Griffith's books can be found here:
All her 18 Audible.com audio books here:
Personal Site:


text:
A.R. lodge and Guest Rooms
Call us 1800-300-2400-1 (Toll Free) and Get additional Discount upto 50% for this Hotel.
You are getting lowest possible rate for this hotel
- About Hotel
- Reviews
About A.R. lodge and Guest Rooms
Offering accommodation in Bangalore, A.R. lodge and Guest Rooms is accessible from Manchenahalli Bus Stand (0.5 km) and Heelalige Railway Station (12 km). The property is inclusive of a front desk that serves 24-hour to its patrons. There are in total 25 well-appointed rooms that are equipped with essential requisites. Available amenities in the rooms include television set, wardrobe, makup mirror and attached bathroom with hot/cold running water.
No of floors: 4 | No of rooms: 25
Checkin Time: 1200 pm
Checkout Time: 1200 pm
Hotel Amenities
- Laundry Facilities
- 24-Hour Front Desk
- Bottled/drinking Water
- Temperature Control
- Balcony


text:
AT&T officials said on Tuesday that the company has signed on to build and manage a global IP network for General Motors in a contract worth $350 million.
The automaker's multiple networks will be replaced with a network set up by AT&T Solutions, the carrier's professional services subsidiary, according to a joint statement from the companies.
GM and AT&T Solutions will also standardize and manage future development of GM's IT infrastructure throughout its global operations, according to the statement. Moreover, AT&T Solutions will deploy networking capabilities that allow GM teams to work together in design facilities in Michigan, Brazil, Germany, and Singapore.
AT&T also announced Tuesday that it would provide similar services to Delphi Automotive Systems, one of the world's largest automotive systems and components companies, for $160 million.
For GM, AT&T Solutions will work closely with Electronic Data Systems (EDS), GM's current primary network and computer-services provider, to ensure continued operation of legacy systems while the next-generation IP network is being built.
The network will provide connectivity among GM's 1,100 locations in more than 40 countries.
The telephone company will also provide GM with domestic and international inbound and outbound long-distance voice, data and video services, local phone service via AT&T Digital Link, and corporate calling cards.
AT&T Solutions will use the services of Concert, the joint venture between AT&T and British Telecommunications, which will provide global transport for voice and data services. Together, AT&T Solutions and British Telecom Syncordia Solutions (BTSS), British Telecom's network outsourcing subsidiary, will provide managed data, voice, and video services in Europe, Asia, and Latin America. BTSS also will manage relationships with local phone companies overseas.
GM's new network will utilize IP-over-SONet, or Synchronous Optical Network, technology -- including voice-over-IP transmission -- at GM's five-building headquarters in the Detroit-Warren, Michigan, area.
AT&T will engineer the network to interface with Automotive Network eXchange, an IP-based extranet created by the automotive industry to enable electronic commerce and data exchange, officials said.
AT&T Solutions will manage GM's networking capabilities from its Global Client Support Center in Dublin, Ohio, via the AT&T Solutions Global Enterprise Management System (GEMS). GEMS offers network management capabilities -- including predictive management -- by integrating proprietary software developed at AT&T Labs with third-party software.
AT&T Corp., in Basking Ridge, N.J., can be reached at www.att.com. General Motors Corp., in Dearborn, Mich., is at www.gm.com.
This story, "AT&T to build $350 million global IP network for GM" was originally published by InfoWorld.


text:
Here is your weekly ad and coupon matchups for the Whole Foods Market South Weekly Ad that runs from 5/27-6/2/15.
You can view the full Whole Foods coupon policy here. Here are a few highlights: you can combine a store and manufacturer's coupon on the same item. Multiple store coupons are allowed when you buy identical items as long as you have a physical coupon for each one you purchase. Coupons are not doubled or tripled and no overage is given when the coupon value exceeds the price of an item. Case discounts can be combined with coupons.
Third Street Chai Bottled Organic Teas – B1G1
Bread & Bakery
Jalapeno Cheddar Bread – $3.00
Cookies, Snacks & Candy
Annie's Homegrown Cheddar Bunnies – $3.00
Good Health Olive Oil Kettle Chips – B1G1
- $1.00/2 Good Health Products, 3.5 oz or Larger – 1-4-15 RP (exp. 06/30/15)
Greens Plus Bar Energy Chocolate – B1G1
Raspberries, Organic, 6 oz – $3.00
Includes: Driscoll's
- $0.50/1 Driscolls Berries; Must Be Advisory Panel Member and Have Access Code From Survey For Coupon
Final Price: $2.50
Tomatoes, Roma, Organic, per lb – $1.49
Out-of-Pocket Price: $1.49
Final Price: $1.29
Buy 1 pound. Pay $1.49 OOP. Submit for $0.20 rebate. Final Price: $1.29
Never miss another hot coupon, deal or freebie again! "Like" Couponaholic .NET on Facebook, Follow us on Pinterest, Follow us on Twitter, Follow us on Instagram or Subscribe to our Daily Email Newsletter!


text:
If you would like to ship your items to a different country, please select it below. Item availability, prices and shipping will be updated to reflect that country.
Please select the language that you would like to browse the site in.
Please select the currency that you would like to pay with.
Style: 38341061 Colour:
Wool felt hat with a floppy brim and a slouchy silhouette. Adjustable inner band for an easy, effortless fit.
Down Under Floppy Hat styled by the FP Me community
Hey you!
Be the first to write a review.


text:
Adebanji Alade is one of the most exciting new artists in Britain today working in both oil and acrylic. He has won a clutch of awards for his dynamic, mostly urban, paintings that are usually full of people and life. Not that Adebanji paints his people with solemnity and precision. Often as not they are applied in a frenzy of colour and brushstrokes and are little more than dabs of colour. But the results are spectacular and it is this highly energised whirlwind style of painting that he demonstrates on this his first teaching DVD.
On this DVD he travels to the quintessentially English seaside resort of Cromer on the north coast of Norfolk. The DVD was filmed in the middle of summer and Cromer was teaming with holidaymaking families making it ideal territory for Adebanji. He paints his first two pictures on Cromer's Edwardian Pier before moving along the promenade a little to capture an amazing view of the pier and promenade from a high vantage point complete with scores of bustling visitors. He finishes up with a beach scene complete with fishing boats.
Artist: Adebanji Alade
Duration: 133 minutes
Format: English, PAL
To view a trailer for this DVD please click below:
|No questions yet. Be the first to ask the question!
|Books & DVDs : Subject
|Books & DVDs : Medium
|Books & DVDs : Author/Artist
|Adebanji Alade Prov
|To use with
There are currently no reviews for this product.
Write Your Own Review
If you have used this product and would like to share your experience with other artists, please log in to leave a review


text:
Identification and analysis of immune-related transcriptome in Asian seabass
Fish diseases caused by pathogens are limiting their production and trade, affecting the economy generated by aquaculture. Innate immunity system is the first line of host defense in opposing pathogenic organisms or any other foreign material. For identification of immune-related genes in Asian seabass
Sequencing and bioinformatic analysis of 3351 ESTs from two SSH libraries yielded 1692 unique transcripts. Of which, 618 transcripts were unknown/novel genes and the remaining 1074 were similar to 743 known genes and 105 unannotated mRNA sequences available in public databases. A total of 161 transcripts were classified to the category "response to stimulus" and 115 to "immune system process". We identified 25 significantly up-regulated genes (including 2 unknown transcripts) and 4 down-regulated genes associated with immune-related processes upon challenge with LPS. Quantitative real-time PCR confirmed the differential expression of these genes after LPS challenge.
The present study identified 1692 unique transcripts upon LPS challenge for the first time in Asian seabass by using SSH, sequencing and bioinformatic analysis. Some of the identified transcripts are vertebrate homologues and others are hitherto unreported putative defence proteins. The obtained immune-related genes may allow for a better understanding of immunity in Asian seabass, carrying out detailed functional analysis of these genes and developing strategies for efficient immune protection against infections in Asian seabass.
Fish diseases caused by viruses, bacteria and parasites are recognized as a significant constraint on aquaculture production and trade hence affecting the economy seriously
Expressed sequence tags (ESTs) generated by cDNA cloning have proven to be a powerful and rapid tool for identifying genes
The Asian seabass
Immune response can be experimentally stimulated by bacterial lipopolysaccharides (LPS)
SSH efficiency
The efficiency of subtraction can be estimated with PCR analysis by comparing the abundance of housekeeping genes and differentially expressed genes before and after subtraction (Clontech manual). In this experiment, an immune-related gene (the immunoglobulin heavy constant mu: IGHM) and a house-keeping gene (ί-actin) were used to examine the efficiency of subtraction. Quantitative real-time PCR analysis of IGHM and ί-actin revealed that SSH efficiency was 3.4 fold for the forward subtractive library and 4.7 fold for the reverse subtractive library.
Generation of subtractive cDNA libraries and assembly of ESTs
Two subtractive cDNA libraries, a forward subtractive library (genes expected to be up-regulated in response to immune challenge in this library) and a reverse subtractive library (genes expected to be down-regulated in response to immune challenge) were constructed using subtractive cDNA from spleen of Asian seabass sampled at 24 hour post challenge with LPS and control samples. A total of 1527 and 1824 randomly picked clones for the forward subtractive library and the reverse subtractive library, respectively, were sequenced. After trimming of end and vector sequences and eliminating sequences with low quality and/or shorter than 100 bases, a total of 2887 high quality sequences were obtained. Among the 2887 high quality sequences 1168 were derived from the forward subtractive library whereas 1719 from the reverse subtractive library (Table
Table 1
EST sequences and assembly statistics for two suppression subtractive hybridization libraries constructed from spleen cDNA of Asian seabass
Forward subtractive
Reverse subtractive library
Sequences combined
Total number of sequences
Clean sequences (>100 bp)
Low quality sequences
Total number of unique sequences
2 ESTs
3-4 ESTs
≥5 ESTs
SNP-contained contigs
Microsatellite -contained unique sequences (≥8 repeats)
After assembly and clustering, 1692 unique transcripts consisting of 1207 singletons and 485 contigs (Table
Annotation of ESTs
Blast searches against known sequences in public databases using the programs BLASTx and BLASTn revealed that 1074 unique transcripts were similar to 743 known genes and 105 unannotated mRNA sequences with high confidence (E value < 10-4) in the database. The remaining 618 unique transcripts were potentially novel sequences or UTRs of known genes. Three hundred and thirty-one of these known genes and unannotated mRNA sequences were represented by multiple sequences (see Additional file
Additional file 1
Table S1. Summary of BLAST searches of the Asian seabass ESTs from subtractive libraries.
Click here for file
The globin gene family was among the predominant EST sequence clusters in both libraries. Of the 2887 EST sequences, 13.8% (397) matched the globin family (hbaa1, ba1, hbb, Hba-x), 5.7% (165) were ribosomal proteins (12 S, 16 S, 18 S, 40 S and 60 s), 1.6% (47) were initiation factors and 1.2% (34) belonged to elongation factors (Additional file
The complete results of BLAST searches of the Asian seabass ESTs from the forward subtractive and reverse subtractive libraries are summarized in Additional file
Functional classification
Available bioinformatic tools, such as the Gene Ontology (GO) GO Slimmer and Kyoto Encyclopedia of Genes and Genomes (KEGG) provide useful information to analyze the functional profile of annotated genes
Additional file 2
Fig. S1. Classification of 743 annotated genes from Asian seabass in subcategories of biological process following GO.
Click here for file
Additional file 3
Fig. S2. Classification of 743 annotated genes from Asian seabass in subcategories of cellular component following GO.
Click here for file
Additional file 4
Fig. S3. Classification of 743 annotated genes from Asian seabass in subcategories of molecular function following GO.
Click here for file
Additional file 5
Table S2. Functional classification of the Asian seabass genes from the reverse subtracted library following GO.
Click here for file
Additional file 6
Table S3. Functional classification of the Asian seabass genes from the forward subtracted library following GO.
Click here for file
KO (KEGG Orthology) is a pathway-based classification of orthologous gene groups. Four hundred and sixty seven of the 1692 unique transcripts with KO assignments were composed of 402 unique genes. Of which, 257 unique genes belonged to 160 KEGG pathways. Interestingly, 52 annotated genes were found in 10 immune-related pathways, including complement and coagulation cascades (13 hits), chemokine signaling pathway (12 hits), antigen processing and presentation (10 hits), Toll-like receptor signaling pathway (9 hits), leukocyte transendothelial migration (8 hits), T cell receptor signaling pathway (7 hits), hematopoietic cell lineage (6 hits), B cell receptor signaling pathway (5 hits), natural killer cell mediated cytotoxicity (4 hits) and Fc epsilon RI signaling pathway (3 hits). Most of the annotated genes were associated with cellular process (213 hits) and diseases (171 hits) and metabolism (167 hits), followed by environmental (79 hits) and genetic (70 hits) information processing. Our data suggest that several important immune processes were likely to be affected by the LPS challenge.
Immune-related genes and differentially expressed genes in subtractive libraries
Genes in the GO subcategories 'response to stimulus' and 'immune system process' are likely to be involved in the interaction of bacteria with its host. We identified a total of 161 genes (78 in the forward subtractive library and 111 in the reverse subtractive library) with response to the LPS challenge, whereas only 28 genes were shared between two libraries. These genes were mainly associated with response to stress (81), response to chemical stimulus (80), response to external stimulus (48), cellular response to stimulus (40), regulation of response to stimulus (35) and immune response (36). Most of the responsive genes were under represented in the library with only one copy in each library. Although the percentage of unique genes responding to stimulus contained in both libraries was not significant, the actual number of the EST clones representing these genes (396 in the forward subtractive library and 384 in the reverse subtractive library) was quite different between two libraries (
Based on the number of homologous ESTs in the two libraries, Fisher's exact test found a significant increase in abundance for a total of 25 genes (including 2 unknown genes) and a significant decrease in abundance for 4 genes upon challenge with LPS, suggesting a strong transcriptional regulation upon LPS challenge (Table
Table 2
Immune response-related genes differentially expressed in Asian seabass at 24 h post challenge by bacterial lipopolysaccharides (
Cluster ID
Gene ID and description
Up-regulated genes
BA1; ba1 globin (
HBAA1; hemoglobin alpha adult-1 (
C1QL4L; complement component 1, q subcomponent-like 4 like (
LY6D; lymphocyte antigen 6 complex, locus D (
IGH-1B; immunoglobulin heavy chain 1b (
C3; complement component 3 (
SAA3; serum amyloid A 3 (
APOE; apolipoprotein E (
NCAN; neurocan (
RPS7; 40 S ribosomal protein S7 (
RRT15; uncharacterized protein YLR162W-A (
AGC1; aggrecan 1(
FGG; fibrinogen, gamma polypeptide (
HDR; hematopoietic death receptor (
LCP1; lymphocyte cytosolic protein 1 (
LGMN; legumain (
APO; apolipoprotein (
B2M; beta-2 microglobulin (
CFL2; cofilin-2 (
GUK1;guanylate kinase (
mt-ND5; NADH-ubiquinone oxidoreductase chain 5 (
TKTL2; transketolase-like protein 2 (
WBP2; WW domain-binding protein 2 (
Unknown mRNA sequence
Unknown mRNA sequence
Down-regulated genes
HMOX1; heme oxygenase (decycling) 1 (
EEF2L; eukaryotic translation elongation factor 2, like (
DBPHT; DNA binding protein with his-thr domain (
EEF; eukaryotic translation elongation factor (
Count-Up, EST count of a given gene in the forward subtractive library; EST count in the reverse subtractive library.
By the GO classification scheme, the significantly differentially expressed genes were divided into subsets based on their functions. Among the genes with increased abundance, 6 major groups can be identified, including binding (IGH-1B, C3, APOE, WBP2, B2 M, LY6 D, CFL2, LCP1, FGG, RPS7), catalytic activity (LGMN, mt-ND5, GUK1), transporter activity (APOE and AGC1), enzyme regulator (APOE, C1QL4L), antioxidant activity (APOE) and structural molecule activity (LCP1 and RPS7) and with no hit for the remaining 10 genes (BA1, SAA3, NCAN, HDR, APO, TKTL2, HBAA1, RRT15 and 2 unknown genes). For the abundance-decreased genes, GO annotation reveals their involvement in several subcategories of molecular function including binding (HMOX1, DBPHT1), catalytic activity (HMOX1) and molecular transducer activity (HMOX1) and no assignment for two remaining genes (EEF, EEF2L).
ESTs containing microsatellites and SNPs
Screening of all EST sequences for microsatellites (2-6 bp) identified 18 microsatellites with ≥8 repeats (Additional file
Additional file 7
Table S4. Summary of unique sequences containing microsatellites in two subtractive libraries of Asian seabass.
Click here for file
Validation of subtractive library data by quantitative RT-PCR (qRT-PCR) and expression profiles of genes in response to LPS challenges
To validate the subtractive library data, qRT-PCR was performed on 13 randomly selected annotated genes and 2 unknown gene sequences. Of which, 9 genes were considered to be up-regulated and 6 genes were presumably down-regulated in spleen upon LPS challenge, based on the differences of homologous EST counts in both libraries (Fig.
Figure 1
The EST counts in two libraries for selected genes used in quantitative RT-PCR analysis
The EST counts in two libraries for selected genes used in quantitative RT-PCR analysis.
Figure 2
Analysis of gene expression in spleen, liver and kidney of Asian seabass by quantitative RT-PCR
Analysis of gene expression in spleen, liver and kidney of Asian seabass by quantitative RT-PCR. Expression levels in three tissues were normalized using EF1A gene as the reference gene. Normalized fold expression data (mean ± s.e.) represented the average of three independent experiments. A star indicated that the expression of the corresponding gene was significantly different (
In addition to the quantitative analysis of the expressions of these genes in spleen by qRT-PCR, expressions of these genes in liver and kidney in experimental (at 24 h post challenge with LPS) and control groups were analyzed using qRT-PCR to add information about the spatial expression of these genes. As shown in Fig.
Discovery of novel ESTs from the subtractive cDNA libraries of Asian seabass spleen
SSH is an effective method to study functional expression levels of specific transcripts in various cells and tissues
Currently, total EST collection for the Asian seabass in NCBI EST database
Genes involved in innate immune responses
Fish represents the earliest class of vertebrates possessing the elements of both innate and acquired immunity
Many pro-inflammatory cytokines such as tumor necrosis factor (TNF)-like, interleukin-1 (IL-1) and IL-1-like receptors have been identified in a number of fish species
Lectins are also known to play an important role in the innate immunity of fish
Differentially expressed genes associated with immune processes
A number of similarities in immune response exist between mammals and fish
Our data also suggest that 8 (HDR, BA1, AGC1, HBAA1, RPS7, EEF and two unknown genes) out of the 29 significantly differentially expressed transcripts could not be identified as immune response-related genes in current public databases. The hematopoietic death receptor (ZH-DR) and the BA1 globin gene were highly differentially expressed upon LPS challenge. ZH-DR was the first TNFR family member to be identified in zebrafish and specific to haematopoietic tissue
Putative markers in innate immunity
The development of fish comparative immunology has been hampered by the lack of specific markers for immunoregulatory peptides
Microsatellites and SNPs in immune-related ESTs
In this study, we identified 18 microsatellites and over 300 SNPs in immune-related ESTs. These markers could be used for many genetic studies
By challenging Asian seabass with bacterial lipopolysaccharide (LPS) and using suppression subtractive hybridization (SSH) technique and bioinformatic tools, we identified a large number of potential immune-related genes. Some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. These genes will supply us a solid basis for a better understanding of immunity in Asian seabass, for conducting detailed functional analysis of these genes and for developing effective strategies for immune protection against infections in the Asian seabass.
Around 100 small Asian seabass at the age of 15 dph were transported from a commercial fish farm to TLL animal house. The fish were maintained in a large tank containing 500 L seawater at 25°C for acclimatization of 3 weeks. Fish were fed twice daily with palliated feed.
Challenging with LPS and sampling
One day prior to challenge, 16 healthy fish individuals of average weight of 5 g were transferred to two smaller tanks holding 10 L of sea water. For 8 fishes in tank 1, each fish was injected intra-peritoneally with 0.1 ml of 2 mg/ml of
RNA isolation
Total RNA from spleen was isolated using the TRIZOL kit (Invitrogen, Carlsbad, USA) according to the manufacturer's instructions. Purification of mRNA from total RNA was carried out using Oligotex mRNA Midi Kit (Qiagen, Valencia, USA). The concentration and purity of mRNA were measured using a NanoDrop Spectrophotometer, ND-1000 (NanoDrop Technologies, Wilmington, USA).
Subtractive library construction and sequencing of clones
cDNA suppression subtractive libraries enriched for differentially expressed genes were constructed using PCR-Select cDNA subtraction kit (Clontech, Mountain view, USA) according to the manufacturer's protocol. Isolation of pure poly A+ mRNA from total RNA was performed using Oligotex® mRNA Mini Kit (Qiagen, Valencia, USA) according to the manufacturer's protocol. The resulting mRNA from 8 LPS-challenged fishes and the mRNA from 8 PBS-treated fishes (control group) was mixed in equal quantity separately. The forward and reverse subtraction experiments of 1 μg of the mixed spleen mRNA were performed for both samples. The efficiency of subtraction was estimated by comparing the abundance of known cDNA (ί-actin and IGHM) before and after subtraction with quantitative RT-PCR. The PCR products of subtractive cDNA were directly inserted into a pGEM-T vector (Promega, Madison, USA) and transformed into
Sequence analysis and functional annotation
Base calling from chromatogram traces and trimming of vector and adaptor sequences and low-quality regions from EST sequences were performed by using commercial software Sequencher 4.9 (Gene Codes, Ann arbor, MI, USA). Then, high quality ESTs (≥100 bp) of both forward and reverse subtractive libraries were used to form contigs. These contigs were manually revised to detect possible errors. Singletons and consensus sequences of each contig were referred to as unique sequences and were compared against the gene ontology database
Mining of microsatellites and SNPs
All of the unique sequences were searched for microsatellites using the program Tandem Repeats Finder (ver. 4.00)
Statistical analysis
A web tool IDEG6 was used for detection of differentially expressed genes between two libraries using Fisher exact test
Analysis of gene expression by quantitative real-time PCR
Spleen, liver and kidney samples were collected from three seabass individuals challenged with LPS and three individuals from the control group (only injected with PBS, see details in the section "Challenging with LPS and sampling") at 24 h post challenges. Total RNA was isolated using the TRIZOL kit (Invitrogen, Carlsbad, USA) according to the manufacturer's instructions. After DNase treatment with DNase I recombinant (Roche, Branchburg, USA) and purified by phenol-chroform, around 1 μg aliquot of the DNase-treated total RNA were reverse transcribed to cDNA by M-MLV reverse transcriptase (Promega, Madison, USA) with 0.67 μM poly dT as RT primer in 15 μl volume following the manufacturer's protocol. The reaction mixture of the RNA template and RT primer was heated at 70°C for 5 min to denature the RNA and then incubated on ice for 5 min. The remaining reagents were added as specified in the thermoscript protocol and the reaction proceeded for 1 hour at 42°C. Finally, the reverse transcriptase was inactivated by incubation at 70°C for 15 min.
For the analysis of expression patterns, the resulting single strand cDNA were 10 times diluted and assayed as DNA template by real-time PCR using primers (see Additional file
Additional file 8
Table S5. Primers used in the analysis of gene expression in spleen, liver and kidney of Asian seabass by quantitative RT-PCR.
Click here for file
Additional file 9
Lca-SSH qRT-PCR MIQE checklist. The qRT-PCR experiment report.
Click here for file
All procedures conducted with Asian seabass fish were adhered to animal care guidelines (Guidelines on the Care and Use of Animals for Scientific Purposes) as outlined by the National Advisory Committee For Laboratory Animal Research in Singapore. An approval is not required as both authors have the certificate of responsible care and use of laboratory animals. The remaining fish were used for other experiments, e.g., construction of cDNA libraries, isolation and identification of microRNAs.
SNP: single-nucleotide polymorphism; EST: expressed sequence tag; SSH: suppression subtractive hybridization; LPS: bacterial lipopolysaccharides; UTR: untranslated region; GO: gene ontology; KEGG: kyoto encyclopedia of genes and genomes; qRT-PCR: quantitative real-time polymerase chain reaction and MIQE: Minimum Information for Publication of Quantitative Real-Time PCR Experiments.
Authors' contributions
GHY initiated and overviewed the Asian seabass project and finalized the manuscript. JHX designed, carried out the study and drafted of the manuscript. Both authors have read and approved the final manuscript.
This study is funded by Ministry of National Development for the project "To ensure self-sufficiency of safe seafood for Singapore through the development of aquaculture genomic tools for marker-assisted selective breeding of tropical marine foodfish". We thank our colleagues Dr Mamta Chauhan and Ms Rahila Qureshi for editing English of this paper.
Assessment of freshwater fish seed resources for sustainable aquaculture
Probiotic bacteria as biological control agents in aquaculture
Enright WJ: Health management practices for cage aquaculture in Asia - a key component for sustainability
Innate host defense mechanisms of fish against viruses and bacteria
A review on the interactions between gut microbiota and innate immunity of fish
Bacterial triggering of inflammation by intracellular sensors
The innate immune response of finfish: A review of current knowledge
Reduction of antibacterial drug use in Norwegian fish farming due to vaccination
Finfish immunology and its use in preventing infectious diseases in cultured finfish
Vaccine development for Asian Aquaculture
A protective immune response is generated in rainbow trout by an OmpH-Like surface antigen (P18) of
A catalogue of genes in the cardiovascular system as identified by expressed sequence tags (ESTs)
Comprehensive EST analysis of Atlantic halibut (
Identification of Estrogen-Responsive Genes in the Testis of Sea Bream (
An extensive resource of single nucleotide polymorphism markers associated with Atlantci Salmo (
Single nucleotide polymorphism discovery in rainbow trout by deep sequencing of a reduced representation library
Assembly of 500,000 inter-specific catfish expressed sequence tags and large scale gene-associated marker development for whole genome association studies
Genomic resources for a commercial flatfish, the Senegalese sole (
Profiling of infection specific mRNA transcripts of the European seabass
Characterization of an Atlantic cod (
Identification of viral induced genes in Ig+ leucocytes of Japanese flounder
Analysis of the immune-related transcriptome of a lophotrochozoan model, the marine annelid
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries
Differential expression of three
Molecular identification and expression of differentially regulated genes of the european flounder,
Immune-relevant (including acute phase) genes identified in the livers of rainbow trout,
Identification of immune-relevant genes from Atlantic salmon using suppression subtractive hybridization
Gene expression profiles of some immune relevant genes from skin of susceptible and responding Atlantic salmon (
Genes associated with an effective host response by Chinook salmon to
Identification of differentially expressed genes in lipopolysaccharide-stimulated yellow grouper Epinephelus awoara spleen
Characterization of the BPI-like gene from a subtracted cDNA library of large yellow croaker (
Functional genomic analysis of the response of Atlantic cod (
Suppression subtraction hybridization (SSH) and macroarray techniques reveal differential gene expression profiles in brain of sea bream infected with nodavirus
Identification of immune-related genes from kidney and spleen of turbot,
Molecular cloning and preliminary expression analysis of banded dogfish (
Nodavirus infection of sea bass (
A Microsatellite Linkage Map of Barramundi,
Aquaculture: Principles and Practices
Genetic analyses of Asian seabass stocks using novel polymorphic microsatellites
Characterization of two parvalbumin genes and their association with growth traits in Asian seabass (
Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi,
Quarantine and Fish Diseases in Southeast Asia. Report of Workshop
Quarantine and Fish Diseases in Southeast Asia. Report of Workshop
Immune responses of barramundi,
Elements of immunity -The instructive role of innate immunity in the acquired immune response
Innate immunity: Impact on the adaptive immune response
Bacterial lipopolysaccharides induce genes involved in the innate immune response in embryos of the zebrafish (
Singapore grouper iridorirus (SGIV) infected grouper revealed by suppression subtractive hybridization-reverse Library
Expressed sequence tags (ESTs) from immune tissues of turbot (
AmiGO Hub, Web Presence Working Group. AmiGO: online access to ontology and annotation data
Complementary DNA sequencing: expressed sequence tags and human genome project
Identification and analysis of a prepro-chicken gonadotropin releasing hormone II (preprocGnRH-II) precursor in the Asian seabass,
Phytogeny of immune responsiveness: Marine invertebrates
The immunoglobulin genes of fish
Comparison of select innate immune mechanisms of fish and mammals
Cell markers and determinants in fish immunology
Cloning, Characterization, and Expression Analysis of Hepcidin Gene from Red Sea Bream (
Molecular cloning and linkage analysis of the Japanese medaka fish complement Bf/C2 gene
Complete sequencing and expression of three complement components, C1r, C4 and C1 inhibitor, of the classical activation pathway of the complement system in rainbow trout
Three isoforms of complement properdin factor P in trout: Cloning, expression, gene organization and constrained modeling
Identification, mapping, and genomic structural analysis of an immunoreceptor tyrosine-based inhibition motif-bearing C-type lectin from homozygous clones of rainbow trout (
Cloning, mapping and genomic organization of a fish C-type lectin gene from homozygous clones of rainbow trout (
Genes encoding putative natural killer cell C-type lectin receptors in teleostean fishes
The dorsoventral regulatory gene cassette spatzle/toll/cactus controls the potent antifungal response in Drosophila adults
A history of fish immunology and vaccination I. The early days
Transcriptome profiling and functional analyses of the zebrafish embryonic innate immune response to Salmonella infection
Apolipoprotein E modulates immune activation by acting on the antigen-presenting cell
Stimulation of erythropoiesis by inhibiting a new hematopoietic death receptor in transgenic zebrafish
Death receptor signaling and its function in the immune system
Hemoglobin induction in mouse macrophages
Globin attenuates the innate immune response to endotoxin
Reduced stress defense in heme oxygenase 1-deficientcells
The co-ordinated regulation of iron homeostasis in murine macrophages limits the availability of iron for intracellular
Local and systemic gene expression responses of Atlantic salmon (
Characterization of macrophages from the bony fish gilthead seabream using an antibody against the macrophage colony-stimulating factor receptor
Microsatellites and their genomic distribution, evolution, function and applications: A review with special reference to fish genetics
DNA marker technologies and their applications in aquaculture genetics
Genetic variation and population structure of Asian seabass (
Simple sequence repeats in zebra finch (
Efficient detection of parentage in a cultured Japanese flounder
Novel microsatellites from Asian sea bass (
Microsatellites within genes and ESTs of common carp and their applicability in silver crucian carp
Transferability of the EST-SSRs developed on Nules clementine (
KAAS - KEGG Automatic Annotation Server
Antimicrobial Peptide Database
Tandem repeats finder: a program to analyze DNA sequences
IDEG6: a web tool for detection of differentially expressed genes in multiple tag sampling experiments
Independent groups T-TEST for means calculator
Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
Validation of reference genes for real-time polymerase chain reaction studies in Atlantic salmon


text:
There's a new Dove Clinical Ibotta cashback offer and it helps make a nice deal even better over at Walgreens. Through 6/25, the Dove Clinical Antiperspirants are on sale BOGO 50% OFF, plus they are part of the Buy $15 of select Unilever Personal Care, Get 3000 Balance Rewards points deal. Check out this awesome scenario…
At Walgreens through 6/25:
BUY TWO DOVE CLINICAL ANTIPERSPIRANT, on sale BOGO 50% OFF $8.79 ($13.18 for two with sale)
BUY TWO SUAVE BODY WASHES 12 OZ (BUT WATCH FOR 18 OZ BONUS SIZE), reg. price $2
Get 3000 Balance Rewards points (value $3-$3.75) for buying $15 of select Unilever Personal Care
Get 10 Everyday Points for every $1 spent (170 points for this transaction; value $0.17-$0.21)
Get $2 cashback from Ibotta for buying Dove Clinical Antiperspirant
Get $0.10 cashback from MobiSave for buying any Deodorant
Use two $2/1 Dove Clinical Protection Antiperspirant coupons, 6/12 RP insert
and use one BOGO Suave Body Wash coupon, 6/12 RP insert
Price at Register: $11.18, but get 3170 BR points (value up to $3.96) and $2.10 total cashback from the apps
NET PRICE FOR ALL: AS LOW AS $5.12 (like paying $2.56 per Dove Clinical and getting two free Suave!)
Thanks Wild for Wags.Extreme Couponing Tip: You can usually get two prints of most internet coupons. To find them in the easiest possible way, use our preclipped links that take you right to the coupon. Then come back and click the same link to get your 2nd print. This will save you the time spent searching for the coupon.
Never miss another hot coupon, deal or freebie again! "Like" Couponaholic .NET on Facebook, Follow us on Pinterest, Follow us on Twitter, Follow us on Instagram or Subscribe to our Daily Email Newsletter!


text:
We wrote about a few rare Goof Off printable coupons yesterday. After poking around a bit, we found a pic that shows their products priced at $2.97 to $6.97 at Walmart. These products clean up all kinds of things: bubble gum on sidewalkds, tar, graffiti, adhesives, stains and more.
At Walmart:
BUY ONE GOOF OFF PRODUCT, $2.97-$6.97 (varies based on what items you choose)
Use one $1/1 Goof Off Professional Item printable coupon (preclipped)
or use one $1/1 Goof Off Heavy Duty Item printable coupon (preclipped)
Thanks and photo credit to I Heart the Mart.Extreme Couponing Tip: You can usually get two prints of most internet coupons. To find them in the easiest possible way, use our preclipped links that take you right to the coupon. Then come back and click the same link to get your 2nd print. This will save you the time spent searching for the coupon.
Never miss another hot coupon, deal or freebie again! "Like" Couponaholic .NET on Facebook, Follow us on Pinterest, Follow us on Twitter, Follow us on Instagram or Subscribe to our Daily Email Newsletter!


text:
Cong. Ileana Ros-Lehtinen is pleased that the House has passed legislation she has long supported to eliminate the burdensome 3% withholding tax on all government contractors including many small businesses and Medicare providers. The bill was approved by an overwhelming bipartisan majority.
This legislation will prevent a 3% withholding penalty on US government payments to all federal, state and local contractors that were scheduled to go into effect January 1, 2013. Many small businesses were concerned that such a requirement would have wiped out their profit margins and seriously disrupted their cash flow during a serious economic downturn.
Medicare providers also supported the bill because it will prevent disruption of health care to their patients. Such a penalty could have caused substantial costs to doctors and other health care providers which would have harmed their ability to provide vital services.
It will also help universities and colleges, as well as state and local governments who are federal contractors. Florida organizations supporting the bill include the Florida League of Cities and Miami-Dade County.
Said Ros-Lehtinen, "I am pleased that the House approved this bill with strong support from both parties. This is a great solution that frees small businesses to grow and hire more employees. It will benefit the South Florida community including health care providers and local governments."
Source: http://ros-lehtinen.house.gov/press-release/ros-lehtinen-co-sponsored-bill-approved-house-aims-help-small-businesses-medicare


text:
The ad looks and sounds like that Old Spice commercial in which a supremely self-confident guy brags that other men can't match his sex appeal. "Look at yourself. Now back to me. Now back at yourself. Now back to me. Sadly, you are not a monster."
It's not the Old Spice guy. It's Grover, the Muppet from Sesame Street with the strangulated voice, blue fuzz and red nose. If you haven't seen this 40-second promo, which has gone viral on the Web, look for it. It's very funny.
In fact, it's the funniest entry on Sesame Street Spoofs!, a two-disc DVD compilation of old and new Sesame Street parodies of television series, movies and songs. Muppets twist the plots, usually with the help of puns ( The King and I is about the letter "i"), to teach preschoolers to add, pronounce letters and control their emotions. In this version of Mad Men, the characters get really mad.
The first hour-long disc, from the days when Muppets creator Jim Henson was alive and assisted by the likes of Frank Oz, has fun with Casablanca (pianist Sam recites the alphabet) and Madonna's song Material Girl ("Now I am a cereal girl"). On the second, later disc, a parody of True Blood called True Mud begins with a deft parody of the show's swamp-rock theme song. The Closer is about closing briefcases.
With the rare exception (did I mention Grover's Old Spice riff?), the contents elicit smiles rather than laughs. The spoofs hover uneasily between two worlds: the universe of children, who must be taught simple lessons and have never heard of most of the shows being kidded, and the universe of adults. The idea is to slip the grown-ups these parodies as a reward for watching Sesame Street with their kids, but the jokes usually hinge on simple puns.
Many of the spoofs on the first disc are ancient history: Dragnet, This Is Your Life, Family Feud. But the humour is freewheeling, in the style of Henson's more adult-oriented Muppet Show. The Cookie Monster hosts Monsterpiece Theater as Alistair Cookie, introducing Gone with the Wind ("Me not see it yet, but me hear it about the wind"). There's a smart parody of Bruce Springsteen's Born to Run ("Kids like you and me, baby, we were born to add") and of Billy Idol's Rebel Yell (a rebel L just wants "to live for loudness").
The pop-cultural references on the second disc are more numerous, but the humour is tamer; Henson's successors can't quite replicate his off-kilter sensibility. Sometimes a premise goes nowhere, as in an encounter between Dr. Phil and his Muppet doppelganger, Dr. Feel. Sometimes it's inspired, as in a parody of 30 Rock (about the delivery of 30 rocks) in which Liz Lemon is a real lemon. The characters in Law and Order: Special Letters Unit notice and reflect upon the show's distinctive two-note musical sting. ("You know, that 'chung chung' can really get on a person's nerves.")
It's hard to top Grover's Old Spice routine, though. That guy has a great career ahead of him.Report Typo/Error
Follow us on Twitter: @GlobeArts