text:
Next generation sequencing analysis reveals a relationship between rDNA unit diversity and locus number in
Comparative and evolutionary genomics
Tandemly arranged nuclear ribosomal DNA (rDNA), encoding 18S, 5.8S and 26S ribosomal RNA (rRNA), exhibit concerted evolution, a pattern thought to result from the homogenisation of rDNA arrays. However rDNA homogeneity at the single nucleotide polymorphism (SNP) level has not been detailed in organisms with more than a few hundred copies of the rDNA unit. Here we study rDNA complexity in species with arrays consisting of thousands of units.
We examined homogeneity of genic (18S) and non-coding internally transcribed spacer (ITS1) regions of rDNA using Roche 454 and/or Illumina platforms in four angiosperm species,
Results and Conclusions
In all four species the intragenomic homogeneity of the 18S gene was high; a single ribotype makes up over 90% of the genes. However greater variation was observed in the ITS1 region, particularly in species with two or more rDNA loci, where >55% of rDNA units were a single ribotype, with the second most abundant variant accounted for >18% of units. IGS heterogeneity was high in all species. The increased number of ribotypes in ITS1 compared with 18S sequences may reflect rounds of incomplete homogenisation with strong selection for functional genic regions and relaxed selection on ITS1 variants. The relationship between the number of ITS1 ribotypes and the number of rDNA loci leads us to propose that rDNA evolution and complexity is influenced by locus number and/or amplification of orphaned rDNA units at new chromosomal locations.
In most eukaryotes, 5S and 18–5.8–26S nuclear ribosomal DNA (rDNA) units occur in tandem array at one or several loci. Each large rDNA unit (designated 45S and 35S for animals and plants, respectively) contains three different rRNA genes (18S, 5.8S and 26S), which are separated by two internal transcribed spacer sequences (ITS1 and ITS2). Each group is separated by the intergenic spacer (IGS,
Of particular interest to evolutionary biologists is the pattern of divergence of the whole rDNA array known as concerted evolution, in which the units of the rDNA array are very similar within a species but diverge between species. This pattern suggests that the arrays are subject to homogenisation – so that novel variants arising by mutation spread relatively rapidly along the array within any one species
In angiosperms, rDNA evolution has been challenging to study, since there are many copies of the large 35S unit, amounting to thousands, or tens of thousands of units
In this work, we addressed inter- and intragenomic homogeneity of rDNA arrays in four diploid
Plant material
The following accessions were used: i)
Preparation of sequencing amplicons by emulsion PCR
Emulsion PCR was used in all steps in order to prevent formation of chimeric DNA during amplification. We first separated individual rDNA units in genomic DNA by
For the emulsion PCR we essentially followed the protocol of Williams et al.
The composition of the aqueous phase (230 μl) for the emulsion was as follow: 1x PfuUltra II reaction buffer, 10 g l-1 BSA, 300 nM each forward and reverse primer, 200 μM each dNTP, 5 ng μl-1 of template DNA and 4.6 μl of PfuUltra II Fussion HS DNA Polymerase (Stratagene, USA). The amount of genomic DNA was reduced to a minimum in order to maintain an excess of aqueous droplets over template molecules. The tripartite structure of primers was as follows: 454 sequencing adaptors (italics), variable hexanucleotide TAG sequence (normal letter) and a gene specific 3' end (bold). Primer A (5'-
Amplicon sequencing
Samples containing 100–200 ng of each tagged PCR product were pooled and sent to EuroFins Operon MWG (Germany) for 454 sequencing. The pooled sample was sequenced from Primer A- and Primer B sides using a Roche GS FLX apparatus and Titanium series chemistry. Average length of reads was 350 to 450 base pairs and these reads are available at the NCBI Sequence Read Archive under the accession number SRA051350.
Amplicon variance analysis was carried out at Eurofins MWG Operon (Germany) and included Mira coverage mapping and syntheny plots. The SNPs were detected in the 454SNP/454HCDiff.files retrieved from the GsMapper. The criteria for clustering were as follows: All alignments were performed using the Smith-Waterman algorithm with default parameters with an identity threshold of e-100 and a maximum of 10 mutations per read. The reference sequences used for mapping were obtained from direct Sanger sequencing of 18S-5.8S PCR products. In general, half of the reads were successfully mapped to the reference sequences. The unmapped reads contained mostly shorter sequences or unrelated and highly variable sequences that were excluded from further analysis. The lengths of reference sequences were as follows: 313 bp of the 18S (all species), 240±2 bp of the ITS1. The 313 bp of 18S gene is delimited by positions 1689–1801 of the 18S gene from tomato (X51576.1); positions of ITS1 were according to the sequences deposited in GenBank (AJ492423.1, AJ492450.1, AJ492455.1, AJ492445.1,
Illumina genomic DNA sequencing
Sequence reads were obtained from Illumina sequencing of
Analysis of clones
ITS sequences were obtained by PCR amplification of genomic DNA. In a 25 μl reaction we used 0.1–1 ng genomic DNA as template, 500 nM each primer, 200 μM each dNTP, and 0.4 units of DyNAzyme II DNA polymerase (Finnzymes, Espoo, Finland). Cycling conditions were as follows: an initial denaturation step of 94°C for 180 s followed by 15–35 cycles of 94°C for 20 s, 57°C for 30 s and 72°C for 30 s with 18S-FOR (5'-GCGCTACACTGATGTATTCAACGAG-3') and 5.8S-REV (5'-CGCAACTTGCGTTCAAAGACTCGA-3') primers
Southern blot hybridisation
DNA was extracted from fresh young leaves according to Kovarik et al.
Data access
All reads are available at the NCBI Sequence Read Archive (SRA) under the accession numbers SRA051350 and SRA045794.1. The cluster of reads used for mutation analysis can be accessed from:
Figure 1
(A) Structure of 35S rDNA unit in
(A) Structure of 35S rDNA unit in
We used emulsion PCR (for primer sites see Figure
Additional file 1
List of 18S gene clusters from 454 amplicon sequencing. Description: Each MsExcell list contains information about cluster ID, mutation pattern, number of reads, number of reads expressed as a percentage and total number of reads for a given species. For
Click here for file
Additional file 2
List of ITS1 clusters from 454 amplicon sequencing. The MsExcell charts are organized as in Additional file
Click here for file
Additional file 3
Substitution mutation patterns in ITS1 region (single read clusters were excluded).
Click here for file
Additional file 4
Abundance of individual clusters (number of reads/total number of reads per cluster, expressed as a percentage) in 454 sequencing data from
Click here for file
Figure 2
Relative abundance of individual clusters in 454 generated (A) 18S rDNA and (B) ITS1 sequences
Relative abundance of individual clusters in 454 generated (A) 18S rDNA and (B) ITS1 sequences. The clusters are distinguished by SNPs, with clusters carrying indels having been merged with clusters that carry the same SNP profile.
Illumina sequencing
Illumina reads from
Additional file 5
Output of SNP algorithm in CLC genomics of Illumina ITS1 reads from
Click here for file
Additional file 6
Output of DIP algorithm in CLC genomics of Illumina ITS1 reads from
Click here for file
Figure 3
Number of ITS1 variants occurring in each of five abundance categories for
Number of ITS1 variants occurring in each of five abundance categories for
Comparing data between sequencing methods
To validate the results we compared frequencies of SNPs obtained with different sequencing methods across
Additional file 7
Comparison of polymorphisms analyzed by different sequencing methods. The data are for
Click here for file
Among the 3882
Phylogenetic relationships between the ITS1 families
To determine phylogenetic relationships between ITS ribotypes, we extracted a single ITS ribotype from
Figure 4
Maximum likelihood tree showing relationships between major ITS families
Maximum likelihood tree showing relationships between major ITS families. Sequences are from abundant clusters (>5% of reads) derived from Roche 454 sequencing. Branch support is indicated at each node and was calculated using 500 ML replicates. SYL =
Southern blot hybridisation
The intergenic spacer (IGS, Figure
Additional file 8
Representation of IGS sequences in 454 reads. The plots show number of similarity hits along (A)
Click here for file
Figure 5
Southern blot hybridisation shows the presence of multiple IGS variants in the
Southern blot hybridisation shows the presence of multiple IGS variants in the
To study concerted evolution of rDNA arrays during the divergence of diploid
Homogeneity in coding and non-coding regions of the rDNA unit
Most rDNA ribotypes have not survived species divergence in
Analyses of the occurrence and distribution of repetitive DNA sequences
Figure 6
Phylogenetic relationships between species in section
Phylogenetic relationships between species in section
Chromosomal locus separation as a driver of ITS complexity
Large scale analysis of rDNA repeats in yeasts
Why then is there a generally greater number of ribotype variants (Table
Figure 7
A theoretical model to explain rDNA divergence
A theoretical model to explain rDNA divergence. Ribosomal DNA arrays (black box) separated by spacer sequences (black line) with some units carrying mutations (asterisks). Green panel: (A) 35S rDNA locus comprising rDNA arrays with a few mutations. (B) Duplication of the 35S rDNA locus, followed by (C) more rapid intrachromosomal homogenisation than interchromosomal homogenisation, leading to genetic divergence of both arrays and an increase in rDNA complexity. Mechanism would involve unequal recombination and gene conversion
The rDNA variants could have arisen either through the amplification of low copy number units (Figure
1. Low copy numbers of rDNA units (or parts of the unit) can be found dispersed across the
2. New loci and units have been shown to evolve rapidly in early generations of synthetic allopolyploids
3. Transposable elements may be vehicles for rDNA mobility and the generation of orphaned rDNA units across the genome. For example in wheat mobile rDNA sites may be connected with meiotic activity of En/Spm elements
The results here indicate that ITS1 is more heterogeneous than 18S, particularly when more than one rDNA locus is present. Low homogenisation frequency between rDNA arrays at different loci, together with strong selection pressures imposed on coding regions, would allow co-existence of repeats with identical coding but more variable non-coding regions. We suggest that locus duplication or amplification of orphaned rDNA units can be vehicles for rDNA divergence (Figure
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
AK, ARL and RN designed the study. AK, ARL, SRB and RN wrote the paper. RM, JF and MAG carried out most of the molecular biology part of the work. SRB, JM and RN carried out bioinformatic studies. All authors read and approved the final manuscript.
We thank Jana Kubíčková for help with sorting the SNP analysis data. The work was supported by the Czech Science Foundation (P501/10/0208; P501/12/G090; 206/09/1751), Academy of Science of the Czech Republic (RVO68081707), the Czech-French bilateral Barrande program (09150SH), Natural Environment Research Council, UK, and Agence Nationale de la Recherche (ANR) research contract (Association pour la Recherche sur les Nicotianées).
Structural organisation and regulation of transcription by RNA polymerase I of plant nuclear ribosomal genes
Molecular drive: a cohesive mode of species evolution
Finely orchestrated movements: evolution of the ribosomal RNA genes
Concerted evolution of multigene families and homeologous recombination
Unequal crossover and the evolution of multigene families
The mutational load of a multigene family with uniform members
Evolution of a large ribosomal RNA multigene family in filamentous fungi: birth and death of a concerted evolution paradigm
Concerted and birth-and-death evolution of multigene families
Non-concerted ITS evolution in Mammillaria (Cactaceae)
Intra-individual polymorphism in diploid and apomictic polyploid hawkweeds (Hieracium, Lactuceae, Asteraceae): disentangling phylogenetic signal, reticulation, and noise
Recent origin and phylogenetic utility of divergent ITS putative pseudogenes: a case study from Naucleeae (Rubiaceae)
The Relationship between Satellite Deoxyribonucleic Acid, Ribosomal Ribonucleic Acid Gene Redundancy, and Genome Size in Plants
Organisation of the plant genome in chromosomes
Extensive Pyrosequencing Reveals Frequent Intra-Genomic Variations of Internal Transcribed Spacer Regions of Nuclear Ribosomal DNA
Navigating the tip of the genomic iceberg: Next-generation sequencing for plant systematics
Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics
Molecular cytogenetic analyses and phylogenetic studies in the Nicotiana section Tomentosae
Comparative genomics and repetitive sequence divergence in the species of diploid Nicotiana section Alatae
Fall and rise of satellite repeats in allopolyploids of Nicotiana over c. 5 million years
Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing
AFLP analysis of genetic polymorphism and evolutionary relationships among cultivated and wild Nicotiana species
Next generation sequencing reveals evidence of genome downsizing and elimination of paternally derived repetitive DNA sequences in tobacco
Independent, Rapid and Targeted Loss of Highly Repetitive DNA in Natural and Synthetic Allopolyploids of
A physiological and molecular analysis of the genus Nicotiana
Nuclear glutamine synthetase evolution in Nicotiana: Phylogenetics and the origins of allotetraploid and homoploid (diploid) hybrids
Molecular phylogeny of Nicotiana (Solanaceae) based on the nucleotide sequence of the matK gene
Molecular systematics, GISH and the origin of hybrid taxa in Nicotiana (Solanaceae)
The use of combined FISH/GISH in conjunction with DAPI counterstaining to identify chromosomes containing transgene inserts in amphidiploid tobacco
Comparative genomics and repetitive sequence divergence in the species of diploid Nicotiana section Alatae
Dynamic changes in the distribution of a satellite homologous to intergenic 26-18S rDNA spacer in the evolution of Nicotiana
Rapid evolution of parental rDNA in a synthetic tobacco allotetraploid line
Genome evolution in allotetraploid Nicotiana
Elimination and rearrangement of parental rDNA in the allotetraploid
Concerted evolution of 18-5.8-26S rDNA repeats in Nicotiana allotetraploids
Amplification of complex gene libraries by emulsion PCR
Rapid concerted evolution of nuclear ribosomal DNA in two Tragopogon allopolyploids of recent and recurrent origin
BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT
The evolution of ribosomal DNA: divergent paralogues and phylogenetic implications
Ribosomal DNA in the grasshopper Podisma pedestris: escape from concerted evolution
Comparative analysis of DNA methylation in tobacco heterochromatic sequences
Plant rDNA database: ribosomal DNA loci data including other karyological and cytogenetic information in plants
Gene conversion of ribosomal DNA in Nicotiana tabacum is associated with undermethylated, decondensed and probably active gene units
Aggressive assembly of pyrosequencing reads with mates
Nomenclatural changes and a new sectional classification in Nicotiana (Solanaceae)
Highly efficient concerted evolution in the ribosomal DNA repeats: total rDNA repeat variation revealed by whole-genome shotgun sequence data
Sequence variation within the rRNA gene loci of 12 Drosophila species
Intergenic spacer length variants in Old Portuguese bread wheat cultivars
Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics
Molecular organization and evolution of the external transcribed rDNA spacer region in two diploid relatives of
Evolution of the nuclear ribosomal DNA intergenic spacer in four species of the Daphnia pulex complex
Homogenization of Tandemly Repeated Nucleotide-Sequences by Distance-Dependent Nucleotide-Sequence Conversion
Relationships among Nicotiana species revealed by the 5S rDNA spacer sequence and fluorescence in situ hybridization
Phylogenetic relationships in Nicotiana (Solanaceae) inferred from multiple plastid DNA regions
The origin of tobacco' s T genome is tracted to a particular lineage within Nicotiana tomentosiformis (Solanaceae)
Gene trees in species trees
Gene tree discordance, phylogenetic inference and the multispecies coalescent
Phylogenetic analysis of Hordeum (Poaceae) as inferred by nuclear rDNA ITS sequences
Accession-Specific Haplotypes of the Internal Transcribed Spacer Region in Arabidopsis thaliana-A Means for Barcoding Populations
Evolution by reticulation: European dogroses originated by multiple hybridization across the genus Rosa
The asymmetric meiosis in pentaploid dogroses (Rosa sect. Caninae) is associated with a skewed distribution of rRNA gene families in the gametes
Karyotypes, heterochromatin, and physical mapping of 18S-26S rDNA in Cactaceae
Evolutionary implications of permanent odd polyploidy in the stable sexual, pentaploid of Rosa canina L
Geographically localised bursts of ribosomal DNA mobility in the grasshopper Podisma pedestris
Chromosomal homogeneity of Drosophila ribosomal DNA arrays suggests intrachromosomal exchanges drive concerted evolution
Satellite DNA from the Y chromosome of the malaria vector Anopheles gambiae
High intrachromosomal similarity of retrotransposon long terminal repeats: Evidence for homogenization by gene conversion on plant sex chromosomes?
Evolution of R1 and R2 in the rDNA units of the genus Drosophila
Comparison of Ribosomal Dnas of Xenopus-Laevis and Xenopus-Mulleri - Evolution of Tandem Genes
Incomplete homogenization of 18 S ribosomal DNA coding regions in
A ribosomal orphon sequence from Xenopus laevis flanked by novel low copy number repetitive elements
Characterization of a Human Orphon 28 S Ribosomal DNA
Contrasting evolution of a satellite DNA and its ancestral IGS rDNA in Phaseolus (Fabaceae)
Unraveling the sequence dynamics of the formation of genus-specific satellite DNAs in the family solanaceae
Sequence subfamilies of satellite repeats related to rDNA intergenic spacer are differentially amplified on
Chromosomal locus rearrangements are a rapid response to formation of the allotetraploid
Homoeologous shuffling and chromosome compensation maintain genome balance in resynthesized allopolyploid
Immediate unidirectional epigenetic reprogramming of NORs occurs independently of rDNA rearrangements in synthetic and natural forms of a polyploid species
Genetic and epigenetic changes of rDNA in a synthetic allotetraploid
Rapid conversion of rDNA intergenic spacer of diploid mutants of rice derived from gamma-ray irradiated tetraploids
Activity of the En/Spm-like transposons in meiosis as a base for chromosome repatterning in a small, isolated, peripheral population of Aegilops speltoides Tausch
Single integration and spread of a copia-like sequence nested in rDNA intergenic spacers of


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text: Culture 7 May 2007 The real price of cheap food A moving film uncovers the rural world abandoned by new Labour <strong>The Lie of the Land</strong Sign up for our weekly email * Molly Dineen is a brilliant film-maker, but even those of us who count ourselves among her biggest fans thought that her last but one documentary, about Geri Halliwell, was off-key. You knew she'd got too close when she interviewed the former Spice Girl in the loo - an intimacy which, for all that it said plenty about Geri's cloying neediness, made you fear for Dineen, whose films, however involving, have always been so clear-eyed. Luckily, Geri was a blip - a girly aberration. By way of a follow-up, Dineen first made a film about reform of the Lords, which pulled off the great feat of making one care about hereditary peers. Now comes The Lie of the Land (3 May, 9pm), a film populated by the one group of desperately poor for whom the present government seems to have absolutely no sympathy: farmers. Did David Miliband, in the lull after the excitement of his putative Labour leadership bid, settle down to watch? I doubt it. He's too busy talking recycling to worry about the people who produce - or should produce, if only the supermarkets would let them - our food. Dineen did something very simple: she went out into the countryside, found a few of its older and least-privileged inhabitants, and talked to them. Her focus was ostensibly narrow. Having joined a tiny Cornwall hunt - the Cury - her attention was caught by the meat on which the hounds were fed: the carcasses of animals from nearby farms. The next day, she joined the master of the hunt, Ian Williams, as he drove from farm to farm picking up more of these animals - the "flesh run", as it is known. Sometimes they were already dead; sometimes it was part of his job to kill them. For each calf he picked up, he was paid £2. Occasionally, the farmer would also leave him a gift - a bag of fudge on the day Dineen was with him. This business was shocking for two reasons. First, because Ian was often killing a healthy calf simply because it had no market value and the farmer could not afford to keep it. Second, because this business was virtually his sole source of income. Back at his run-down farm, he skinned a calf and remarked out loud that it was a good size. He'd get a few extra pence for the hide. The Lie of the Land didn't do much more than examine the pitiful finances, and the Kafka-esque bureaucracies, of farming in 21st-century Britain. The material was oddly repetitive, one farmer after another explaining how the government now pays them to manage land rather than produce food. But oh, it was powerful. Dineen rehearsed important arguments: about the supermarkets' lust for cheap (and thus unethical and non-British) food; about how if British livestock farming ceases to exist altogether, as is widely predicted, the countryside will physically change for ever. Yet it was her human-interest stories that really moved. I thought about the time when the countryside marchers came to town - when I was tempted to shout at the mass of Barbour-coated oafs who were waddling through Hyde Park: "Get off my land!" - and felt guilty. Here, there were no chinless wonders, no women in sage quilting. These were ordinary people who were clinging on by their fingertips. Ian, in particular, brought a lump to the throat: the archaic way he referred to his broken car window as "he" rather than "it", the way his mum still cooked lunch for him every day in the house where he was born. Dineen didn't try to protect him from the squeamish gaze of the liberal urbanite. We saw him kill, we saw him dismember. In part, this was because she was determined not to sentimentalise the countryside. She wanted us to see things in relative terms: which is worse, fox-hunting or cheap meat? But mostly, I suspect, it was because she knew we would fall for Ian anyway. Why, I wonder, are the rural poor of so little interest to new Labour? Is it that they don't complain? Ian seemed bewildered rather than angry, and it was left to Molly and her camera to articulate the painful story of the ground shifting beneath his feet. Pick of the week Gavin Stamp's Orient Express8 May, 7.15pm, Channel 5Amusingly posh architecture writer traces the train's original route. Return of the TribeStarts 8 May, 8pm, Channel 5The Insect people of Papua New Guinea come to observe Britain. Balderdash and Piffle11 May, 10pm, BBC2Victoria Coren examines the roots of words describing madness. Rachel Cooke trained as a reporter on The Sunday Times. She is now a writer at The Observer. In the 2006 British Press Awards, she was named Interviewer of the Year. Subscribe from just £1 per issue This article first appeared in the 07 May 2007 issue of the New Statesman, Blair: The reckoning More


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- Enorthotrope
- Enorthotrope En*or"tho*trope, n. [Gr. ? in + ? upright, correct + ? to turn.] An optical toy; a card on which confused or imperfect figures are drawn, but which form to the eye regular figures when the card is rapidly revolved. See {Thaumatrope}. [1913 Webster]
The Collaborative International Dictionary of English. 2000.


text:
Once I move one, I can only move it in that same direction and can not move any of the others after that.
The Spiceworks 3.5 Beta Testing forum has been locked.
To continue this discussion, please ask a question in another forum.
5 Replies
Nov 13, 2008 at 2:00 UTC
What browser did you test this in, my FF 3.0.3 works fine. Able to move all enties up and down and back again.
Nov 14, 2008 at 11:53 UTC
I just tried it again and thought it was working but then it starting doing the same thing after moving them around a bit.
This time it is on a fresh install without restored data too.
Nov 14, 2008 at 11:56 UTC
here is a screen shot
Nov 14, 2008 at 12:11 UTC
Since the beta went out, we have noticed some issues with the arrows on IE. There are some changes under way to fix these problems. I would like to know when the entry with Domain Admin dropped below the 'Click here to add a new scan entry'. Was it the first time you arrowed down or did you move the bottom one up?
We will definitely want you to retry this when the next beta drop goes out. You are welcome to keep playing with it but we do know there are some IE issues. Which version of IE are you running?
Nov 14, 2008 at 12:30 UTC
Vista IE7
OK, I think I isolated the problem somewhat.
If you arrow down one of the choices so it is at the bottom, the one on the bottom is fine when the page loads but if you move one to the bottom then the problems start. The whole row becomes left justified and then it will allow you to move it down again under the add a new one text. The top one becomes unmoveable once you move the one currently there down one spot. And it just gets more complicated if you have more than 3 rows you are moving. You can move every row but the top one below the add new one text.


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Is your business in need of specialist health and safety training courses or expert consultancy services? We can provide evacuation safety plans, risk assessment, risk management, Display Screen Equipment (DSE) risk assessments, manual handling training courses, evacuation chair training and 'training for the trainer' courses? Is your business compliant with current HSE legislation? Has your business seen significant change or do your employees need a skills update? If you're looking for a solution to any of these questions, Environmental Handling Systems can help you with our comprehensive r...
23 Helmsdale, Bracknell, RG12 0TA, United Kingdom
Health and safety management consultants, de Silva Safety Services provide a range of training courses and risk assessments to meet safety requirements when at work. Training courses include CAT & genny training, fire training, lock out tag out training, health and safety training, manual handling training, construction site safety, workhouse safety and office safety. We also provide a range safety assessments for work environments from fire risks, computer workstation safety, manual handling, workplace risk assessments and DSE assessments.
20 Stourbridge Road, Halesowen, West Midlands, B63 3US, United Kingdom
Bradley Environmental Consultants Limited offer a wide range of services throughout the UK including Workplace Health and Safety, Health and Safety Audits, Health and Risk Assessments, Noise Monitoring, Legionella Risk Assessments, Asbestos Surveys, Asbestos Management, Asbestos Awareness Training courses, Workplace Exposure Monitoring and Health and Safety Training courses.
Pentrefelin, Amlwch, Anglesey, LL68 9PF, United Kingdom
HSE and CIEH approved training provider, specialising in the delivery of health & safety, first aid, food hygiene, stress awareness, manual handling, risk assessment and train the trainer courses.


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Chicken a La Creme
Recipe by Realtor by day
I found this on another site. We gave it a try and it's really simple and delicious. I had it on the table in no time so it's great for a quick weeknight meal. Try it over biscuits. I'm posting this for ZWT5 for France.
Top Review by Baby Kato
Wow another great meal, by Realtor by day, Chef by night. We enjoyed this tasty, quick and easy to make dish very much. I served it with recipe #410503 and recipe #409348 what an incredible meal it was. It took quite a while for the sauce to thicken, but it was certainly worth the wait. Thank you so much for sharing another winner. I can't wait to try this over homemade buttermilk tea biscuits...oh yum. :)
See Discussion:3 Reviews
Ingredients Nutrition
- 1⁄4 cup butter
- 1 1⁄2 lbs chicken tenderloins (I used regular chicken breasts and cubed them)
- salt and pepper
- 1 pint heavy cream
Directions See How It's Made
- Melt half of the butter in a large skillet over medium-high heat.
- Season chicken tenders with salt and pepper, and saute them in butter until golden on all sides, about 10 minutes.
- Add the remaining butter, reduce heat to medium, and cook for 5 more minutes.
- Pour the heavy cream into the skillet, and simmer gently for 5 minutes, stirring occasionally, until the sauce has thickened and chicken is cooked through.
- Serve with fresh crusty French bread for sopping up the sauce.


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KoDokugumon were minor villains in Digimon Adventures 01 and the children of Dokugumon.
KoDokugumon looks like a smaller version of Dokugumon. They have yellow and black stripes on their abdomen, and eight blue eyes. They also have red fuzz on their head.
Digimon Adventures 01
KoDokugumon were found in Myotismon's castle along with their mother Dokugumon. They attacked the DigiDestined, but when Garurumon Digivolved into WereGarurumon they manage to kill the KoDokugumon and Dokugumon.
So far KoDokugumon have no attacks.


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Global Coffee Trading Article in Daily Coffee News
Service - Integrity - Quality
Global Coffee Trading's team has more than 3 decades of combined experience in the Specialty Coffee industry across the supply chain. Our expertise ranges from applying sustainable growing practices to milling, from quality control to logistics, from finance to barista training. We are your go-to resource for all things coffee. Every step matters and we're here to help.
West Coast (The Annex, CA) - Click Image
Split Bag Offers - Click Image
East Coast (Continental Terminals, NJ) - Click Image


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Dismiss Notice
Welcome To CK5!
Registering is free and easy! Hope to see you on the forums soon.
Score a FREE Membership sticker when you sign up for a Premium Membership and choose the recurring plan.
Need 69-75 pics ASAP for a magizine article.
Discussion in '1969-1972 K5 Blazer | Truck | Suburban' started by CK5, May 1, 2002.


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Who is Jay Wright? If you ask frequent in the greater Philadelphia area, you would much than probable perceive the self response: He ransomed Villanova basketball! Rollie had a terrible run in the 80's, but it was momentary lived. Since the glorious, magic period of 1985, Villanova basketball has been a sequence of ups and downs, principally downs. From mobile card scandals to work changes, Villanova has eventually recovered what they needed, a manager next to vision who brings knowledge domain to final his creed.
Monday night's construction success concluded #1 hierarchic UCONN gave the Nova Nation
something to settle give or take a few. Now Jay Wright is reaping the benefits, late inking a 7-
year extension to keep on on as the Wildcats commandant. Can you of all time bear in mind self
so vehement give or take a few a Big 5 court game team? Sure, St. Joe's had a attractive season
just two telescoped old age ago, attractive the municipal on a March Madness ride that lasted by a long chalk
longer past best expectable. However, this Villanova troop has populace murmuring
"national challenge." Are you serious? As an impartial spectator, chance adequate
to gain some tickets in a Wachovia Suite, what I saw Monday time period just stunned
me. Two bulky weight contenders slugging it out in outlook of a capableness crowd,
playing to a sweepstake at the partially.
The second part of the team game told a distinct story, such close to Ali's pugilism
tactics in the ring, Villanova took UCONN's most favourable lick falling into a double-digit
hole, and later ferociously unguiculate stern into the hobby to heave out a hand-to-hand win. At
least cardinal a thousand fans stormed the court, a recurrent event surge of white, towels someone
waved throughout, jumping up and down necking Mr. Wright and his skilled
group of hoopsters. The area was utopia; the Nova Nation was on top of the international
for one-night, twinkly downward upon the balance of the institute court game world as if to
say.... "We are Nova, we frisk beside 4 guards, and you better recognise."
For the reasons stated, this is why fully grown men, old ladies and brood alike can all
identify near institute court game. Monday's spectator sport is a lot look-alike an giant menage
reunion. The enthralled that exists linking the fan basic and the body is one that is
never broken, unheeding of scandals and losing seasons. This is the discrepancy
between collegial sports and executive sports. Much like-minded the kid in gym who
gets pelted during scheme orb continually, having no hit and miss ever, eventually, he will
hit you and distress you. So for all of you kids and adults who go dwelling near welts departed
from today's corporal coaching activity, basically have a sneaking suspicion that of Villanova. They dispatched the
Huskies stern to Connecticut with a achromatic eye and blood blister ribs. There is hope, and that
hope is named March Madness.


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Star Wars: Episode II - Attack of the Clones (2002) Dual Audio
6.7/10 by 464,523 votes 142 min 15 May 2002 4,315
Ten years after the invasion of Naboo, the galaxy is on the brink of civil war. Under the leadership of a renegade Jedi named Count Dooku, thousands of solar systems threaten to break away from the Galactic Republic. When an assassination attempt is made on Senator Padmé Amidala, the former Queen of Naboo, twenty-year-old Jedi apprentice Anakin Skywalker is assigned to protect her. In the course of his mission, Anakin discovers his love for Padmé as well as his own darker side. Soon, Anakin, Padmé, and Obi-Wan Kenobi are drawn into the heart of the Separatist movement and the beginning of the Clone Wars.
Quality: HD
Country: USA
Language: English
Budget: $120000000 USD
Director: Lucasfilm, George Lucas, James McTeigue
Actor: Alfama Films, Ewan McGregor, Natalie Portman, Hayden Christensen, Ian McDiarmid, Samuel L. Jackson


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|Reviews for Sealed
| Guest chapter 1 . 12/6/2013
This made me happy. I didn't know I wanted Cas a Navy SEAL but now I LOVE IT. Good job :)
| MirkatManor chapter 1 . 6/16/2013
This makes me absurdly happy.
| pizarra en blanco chapter 1 . 10/24/2012
I love it when Cas is all BAMF! He IS A SOLDIER AFTER ALL! Great work!
| ShatteredSig chapter 1 . 10/21/2012
Too cute! Cas being a SEAL is just too awesome for words! Loved it! Nice job!
| lovinlife123 chapter 1 . 10/19/2012
Ok absolutely inlove with this story! I'm surprised you don't have more reviews! Please an you make a sequel?


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Although I haven't been there long I could tell this wiki hasn't been cared for the articles are fine it's just the rest of the wiki that need work. Wetro
None because it is really disorganized
One but am hoping to edit there more.
I don't know because agian its really disorganized and I don't even know who is an admin.
Any other information: I would really like to adopt it because the wiki seems un updated and has no sense of community it is also highly disorganized.
- Hey there, this wiki is a non-Wikia hosted wiki, and is therefore not possible to be adopted. You should read their about page for more information. The Wikia equivalent of this wiki is found here and is currently run by active administrators, but could still use some extra help. Perhaps you could help edit there? 15:33, August 10, 2013 (UTC)
Hi there. Per T3CHNOCIDE, this is not a Wikia site, so I'm afraid we can't help you become an admin there. - Brandon Rhea(talk) 15:46, August 10, 2013 (UTC)
It's actually a very fine wiki... ℬiggestℒeo (ℳeeting ℛoom) 04:05, August 12, 2013 (UTC)


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While I am a little shy at first I am an outgoing person and really enjoy being with friends. I am a very active person. I truly like just getting outdoors and doing new things . My interests are quite broad. From traveling to photography, I always up for trying new things. I picked up running a couple of years ago and got hooked rather quickly. I was amazed by how friendly people in the sport are. But I am just has comfortable being at home reading as being out in a park being active. I really not sure what else to put here, so if you have any questions feel to ask.
I completed my first full marathon, Could have done better and will certainly do so next year when I head back to do a half and a full in one weekend.It will be a challenge, but hey what is life without a few challenges?
Movies are the same way. But i prefer action, suspense, comedy, horror, drama. Really if I get into the plot I will watch it to the end. I also have a habit of watching a movie on tv even if I happen to have it on dvd which means no commericals, I still get stuck watching the tv verison.
And as far as books I will pick up whatever looks interesting and give it a read. I like the classics and I like bestsellers, But if you have something that you think I should read then tell me and I'll give it a read. I also like to write and perhaps one day I will publish my own book. It is slow going but one day I will finish it.
2. Ipod
3. Pineapple in all forms
4. Good conversation
5. Good friends
6. Cell phone( even though some days I would like to leave it at home and have a nice peaceful day)


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Today's successful retailer needs to be nimble when advertising products and services, and there's no better--or cheaper--way to address that challenge than by painting store windows. Window art and copy, when done right, not only attract attention but also create excitement in shoppers for what's going on inside a store. Commercial window signs can drive foot traffic and sales without threatening a retailer's bottom line.
Things You'll Need
- Page layout software
- Paints for use on glass
- Paintbrushes
- Measuring tape
- Window cleaner
- Paint remover
- Clean rags or paper towels
- Painter's tape
Design the sign to be painted by using page layout software (such as Adobe InDesign) or by scetching it out on grid paper.
Prepare the window by removing any existing art, lettering or signage with a paint remover. Next, use a window cleaner and clean, soft rags or paper towels to remove leftover paint remover as well as fingerprints and grime. Make sure no soap film is left behind, and allow the glass to dry completely.
Place painter's tape around the periphery of the window to protect the wood and hardware holding it in place. Alternately, slather the window's periphery with petroleum jelly. Paint won't stick to the jelly, and you can remove the jelly with just the wipe of a cloth.
Transfer the design to the store window. If you used a computer to create the design, take it to a print shop to have the image printed large enough to fit the window so you can trace the design's outline using this template. If you used grid paper, recreate the grid on the window surface, then freehand the letters and images with a grease pencil or chalk.
Paint the sign. Consider applying multiple coats, especially if you painted the sign on the outside of the window.
Clean up any stray paint strokes, splotches or spills using the same tools you used to prepare the window. Then remove the painter's tape or petroleum jelly, and spot clean any fingerprints or smudges.
Tips & Warnings
- Sign up for classes in commercial art at your local community college or technical school if you'd like to improve your artistic abilities or want to learn more about different techniques. Alternately, visit a website (see References) offering a comprehensive program in commercial sign painting and study on your own. Follow the tutorials that come with these programs until you've reached a comfortable skill level. Choose glass-friendly paints. Water-based enamels offer great color options but may not be suitable for store window exteriors and the accompanying exposure to the elements. Test a variety of glass paints, and choose the ones that offer the best quality at the best price.
Related Searches
You May Also Like
- How to Start a Window Painting Business
- How to Wash Storefront Windows
How to Paint Window Stencils
Stencils produce clean, even designs for many different kinds of decorating projects. Although most common for painted wall borders, there is no...
- How to Paint a Plywood Sign
How to Paint Commercial Buildings
Painting commercial buildings requires the proper equipment and skill. You may be asked to paint a commercial building on a professional basis,...
- Types of Window Displays


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|Price incl. VAT
|Price excl. VAT
|Extended warranty + 1 year £4 Extended warranty + 1 year
Multifunction hand blender Electrolux will be your faithful helper in the household. It has two speeds (standard and turbo) and a variety of accessories. Suitable for sauces, chopping nuts, whipped cream and creams.
Looking for a reliable helper in the kitchen as a multifunctional hand blender? Pak vás jistě nadchne tento designový a kvalitní mixér o příkonu 600 W značky Electrolux. It has two speeds (standard and turbo). To actually belongs mixer foot stainless steel mixing container, cutting container, whisk attachment and Ricers potatoes. The cable is 1.2 meters long.
Blender is suitable for sauces, soups, creams, smoothies, mashed potatoes, chopping nuts and much more.
Specifications can be changed without notice. Images are for illustrative purposes only. Retail Price (MSRP) means the manufacturer's/supplier's suggested retail price.
Link to Manufacturer's Website: Enter link to the external review


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Home / navy pride / navy pride collectibles & gifts / navy pride coins & displays /
Navy Pride Coins & Displays
USN Anchors Away Coin
0 Based on 0 reviews
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shipping & returns
additional info
Product information
- This coin commemorates the creation of Anchor's Aweigh and pays tribute to a century-old Navy tradition.
- Rendered in exceptional detail on the obverse are Naval Academy Bandleader Lt. Charles A. Zimmermann and Alfred HartMiles, who composed the lyrics for the song as a Midshipman First Class at the Naval Academy.
- The reverse bearsthe seal of the U.S. Navy.
- Struck in a brass alloy and finished in antique bronze, this coin is accented in traditional Navy and gold enamel.
- Size: 1 3/4 inch (44mm) Round.


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Every year for the last three years during the holiday season, while everyone is beginning to think about what they are thankful for, who they love enough to buy even the smallest gift, and as the excitement of the holidays begins with the $20 faux lit Xmas trees parked outside the drugstores, I am reminded of the sheer amount of human horsepower put behind my son's year to year progress.
November and December are IEP and IPP time, that is Individualized Education Plan and Individualized Personal Plan. It's the time of the annual review of specific educational and personal goals written for Finbar and revised every year along with documented support, persons and agencies responsible for achieving them. IEP's and IPP's are legal documents, mandated by Federal law for all persons with special needs. Doing the necessary assessments, paperwork and meetings can be an exhausting and emotional process most years for all involved, me included. Finbar has his IEP meeting on November29th. I am always dreading the day, even though the meetings have usually gone well. It's just emotional and stressful.
For those who do not have special needs kids, think of the IEP/IPP process as having to go sit at a table with your health insurance company same time every year and explain why you need insurance, what ailments or potential ailments that you need to be covered and convince them (at your expense sometimes) why they should cover these needs. And you can argue that by law they must cover you, but they of course will always try to cut corners and/or may simply refuse. Now simply replace the "insurance plan" with "education and independent life skills plan" and you have the IEP/IPP process.
Also at this time of year, by December, I wonder why I am so spent, and then I remember, oh yeah - organizing Thanksgiving meal, attending school related holiday activities, planning two birthday parties (for my sons), buying birthday presents for my several relatives with birthdays this season, buying presents, buying presents, oh yes, and buying presents for the VILLAGE of people who help support my son. That would be this year - one occupational therapist, one speech language pathologist, one special ed teacher, one classroom aide, two in-home behaviorists...and a teacher. I get off the hook a little this year because we don't have an adaptive P.E. teacher or multiple rotating classroom aides. When it comes to the hoard of folks who have surrounded my son each year, I take my husband's wise words to heart, "you can't say thank you enough times." So presents it is.
Over the years, I think back to certain people who have worked with my son, and I am so grateful for their intervention and presence and dedication at much needed times. We've been lucky. So many families become so frustrated with the revolving door of people coming into their homes and working with their children. Alas many develop antagonistic or un-cooperative relationships. Some parents hear an "expert" telling them what behavioral problems their child has and so the parents resort to denial. Well, that can't be good for anyone especially the child. But I have always treated "Team Finbar" with welcome arms, respect and gratitude, no matter how exhausting the scheduling. I believe this village of people over the years has rallied around my son, first and foremost because of his charisma and charm, secondly because I embrace their presence and thirdly because they are well, good human beings. Here is a shortlist of significant accomplishments that talented people have drawn out of my son:
- Becky got Finbar to do his first pretend play putting out pretend fires around our house
- Becky also got him to engage in back and forth play by having him color a huge piece of plywood that they used hours on end as a car ramp.
- Sarah got him to refer to himself in first person instead of third person "Finbar"
- Sarah N. got Finbar talking to other children
- Serena got his gross motor jumping and climbing skills kickstarted.
- Emily made Finbar make his very first friend.
- Kristen made me feel like I was a good mom :)
- Lorie whispered in my ear on more than one occasion to advocate for Finbar when I was too scared to. She is always on the lookout for him.
- Jeanette got our family going to church again and became a nice mom friend who gave me mom advice.
- Josh taught Finbar how to act "like a cool guy"
- Cece was Finbar's girlfriend
- Jen showed us that Finbar could make it in Sunday school with the right support
- Cat taught Finbar how to put words to his feelings; Shilpa carried on that task
- Jenna got Finbar to dance with his preschool classmates
- Amber took care of preschool things for me/him when we needed her to without me even asking
- Miss Robbins fully includes Finbar and doesn't make me feel like my often disruptive child is unwelcome in a mainstream classroom
- Mrs. R is helping Finbar learn to play "kick" (soccer) at school with the other boys.
- Emily is available more than she needs to be for someone with such a busy schedule. She is determined to teach Finbar how to play nicely with other children. She cuts him no slack :)
There have been many more villagers over the years. Pediatricians, autism doctors, naturopaths, specialised therapists. Some are the same cast of characters that any child would have in his/her village - devoted understanding grandparents, crazy aunts (you know who you are), Nanan, Goomba...and other mothers (and fathers), especially mothers of neurotypical children from playgroups, school or the neighborhood, who have never judged or excluded Finbar (sadly there are many who have). And lest I forget the dozens of mothers of special needs children who have talked openly with me on subjects that most parents never dare to. And then, there are even the teachers at Declan's preschool, sympathizing with me. And finally a host of teachers of extracurricular activities who worked with Finbar's limitations so he could be included (thanks My Gym and Kindermusik!).
I realize as I look back this year, a particularly important triennial assessment year when his official diagnosis of autism spectrum disorder is reassessed, that I and Finbar could never have come this far without them.
GO TEAM FINBAR and HAPPY THANKSGIVING TO YOU ALL :)
PS - I forgot to include my husband. Kinda goes without sayin', he is there all along :)


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Establish Your Word to Your servant, as that which produces reverence for You (Psalm 119:38).
In the United States, we have been abundantly blessed with religious freedom since our founding. It is hard for many of us to imagine a place where Bibles or Christian literature are scarce or not available, or worse yet, being forbidden by the government to own, read or distribute God's Word.
But for seven decades across Russia and the countries that made up the former Soviet Union, that was indeed the case as the atheistic communist government tried to rid the land of Scripture, and above all, faith in God. Even today—two decades after the Soviet collapse—Russian-language Bibles and Christian literature can be very hard to obtain either because of national government restrictions (largely in the Muslim-dominated republics of Central Asia), or the people simply cannot afford to purchase them due to deep poverty.
Through the years, SGA partners have helped provide thousands of Russian-language Bibles, children's Bibles, Christian books, theological works, and other biblically sound materials for distribution by local evangelical churches. While many established titles have been provided for many years, SGA is also translating and printing newer Christian books and theological titles to help disciple individuals and families, and to ground them firmly in the Word of God.
Overflowing with Thanks in Shchelkovo!
October 13th, 2014My wife Lana and I are visiting missionary pastor Vladimir Aliokhin. He and the members of the Shchelkovo Baptist church send their greetings to the SGA family, and to our partners who help make their ministry possible.
New Bibles for Azerbaijan
August 20th, 2014We are thankful to the Lord to announce that more than 300 new Bibles have been printed in the Azeri language for churches in Azerbaijan, a former Soviet republic in the Caucasus Mountains region.
God's Compassion at Work
August 14th, 2014In Ukraine's northwest Volyn region, SGA-sponsored missionary pastor Vladimir Savich has been hard at work sowing the seeds of the Gospel with every opportunity. He is one of the faithful pastors involved in SGA-sponsored Compassion Ministry, distributing food parcels and Christian evangelistic literature to several families—16 adults and 27 children in all.
Open Hands, Open Doors
November 11th, 2013As evangelical churches across the CIS prepare for upcoming Christmas ministries, SGA-sponsored missionary pastor Yevgeny Taran shared his recollection of how God had prepared the way for their fruitful outreaches last Christmas . . .


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Host Sharon Collins introduces viewers to millennials who are ready to take the reins and lead the conservation effort for a new generation. Thursday, July 27 at 8:30 PM
On Second Thought is a one-hour, daily news talk show that airs at 9 a.m. ET weekdays.
The Savannah River Keeper's Gator Fest 2017, Augusta's open-water swim event, is Saturday.
The GHSA state championships for football are right around the corner. There are 16 teams left on the road to the Georgia Dome and by late Saturday night all eight champions for 2016 will be... MORE
GPB Passport is the new member benefit that provides you with extended access to an on-demand library of quality local and national public television programming, including tens of thousands of hours of your favorite programs.
Fri., January 9, 2015 7:11pm
King Center In Atlanta Plans Events To Celebrate MLK Holiday
The Martin Luther King Jr. Center for Nonviolent Social Change has more than a week of events planned to celebrate the upcoming King holiday.
Full Story
Mon., January 16, 2012 12:44pm
Namesake Center Honors King
A packed house remembered Martin Luther King Jr. Monday at the Atlanta church where he preached his message of justice and civil rights. Led by his older sister and his daughter, the service mixed spiritual messages with political ones in celebrating the icon who would've turned 83 Sunday. The key message was that there's still work to do.
Mon., January 17, 2011 12:45pm
Bill Creates Council To Honor King
As the state celebrates the Martin Luther King Junior holiday, some Georgia lawmakers want to establish a board to honor the slain civil rights leader in his home state.
Mon., January 17, 2011 12:28pm
Volunteers Beautify Black Cemetery
Across the state and the country people celebrated Martin Luther King's birth with a national day of service. That includes President Barack Obama who spent the day painting a middle school in the nation's capitol. In Athens hundreds of volunteers turned out to help clean up a historic black cemetery.
Fri., January 14, 2011 9:59am
No Martin Luther King Jr. Holiday For Some Schools
Fannin and Gilmer County will hold school on holiday. Officials say they need to make up for nine snow days. Civil rights leaders not happy.
Mon., January 18, 2010 12:32pm
Rep. Patrick Kennedy Talks Health Care At Augusta MLK Celebration
In Augusta, Congressman Patrick Kennedy was the keynote speaker at the Mart


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How do I find...
Located on Florida's Space Coast, the mission of the Brevard County Parks and Recreation Department is to enhance the quality of life by providing many types and levels of service that reflect the interest and values of citizens and visitors.
Of the many parks and facilities available throughout the county, those that the Department manages include 121 parks, 3 campgrounds, 3 golf courses, 3 nature centers, 3 education centers, 44 beach access sites, 10 school athletic sites, and more than 16,500 managed acres of Environmentally Endangered Lands sanctuaries and conservation areas. The Parks and Recreation Department's website offers residents and visitors a place to discover the unique features and recreational opportunities available throughout our parks.
Brevard County Parks and Recreation is a local government agency in East Central Florida, USA. It serves the nearly 550,000 residents of Brevard County including the following municipalities and communities: Aurantia, Barefoot Bay, Canaveral Groves, Cape Canaveral, Cocoa, Cocoa Beach, Eau Gallie, Floridana Beach, Georgianna, Grant-Valkaria, Indialantic, Indian Harbour Beach, June Park, Malabar, Melbourne, Melbourne Beach, Melbourne Shores, Melbourne Village, Merritt Island, Micco, Mims, Patrick Air Force Base, Palm Bay, Palm Shores, Pineda, Port St. John, Rockledge, Satellite Beach, Scottsmoor, Sharpes, South Patrick, Suntree, Titusville, Viera, as well as local visitors from Orlando, Daytona, Vero Beach and tourists from outside the state.
Many county parks in Brevard preserve the sensitive ecosystem and include endangered lands and threatened species. Educational programs at nature centers focus on awareness of the outdoors and preservation of the environment which includes
the unique Indian River Lagoon, the St. Johns River, lakes, wetlands, maritime hammocks and scrub. Specific parks are a haven for birdwatching and provide a sanctuary for many bird
communities including the osprey, eagle, egret, pelican, and various wildlife including sea turtles and manatees.
Additionally, for those who love the outdoors, Brevard County's parks provide venues for community and regional activities including public golf at three beautiful golf courses (tee times are available at The Savannahs, Spessard Holland, and The Habitat); camping at three unique campgrounds; horse trails; fishing and boating--with access to the waterways by boat ramps at many parks; beach and lake swimming and various venues for watersports including sailing, windsurfing, surfing, rowing, catamaran, canoe, and kayak.
Facilities for other active sports include softball, tennis, volleyball, horseshoes, shuffleboard, and there are many soccer and Little League ballfields. Picnic pavilions and playgrounds are available at various parks for more passive enjoyment.
Brevard County is a popular tourism site with points of interest including the Brevard Zoo, the Archie Carr National Wildlife Refuge, Canaveral National Seashore, Merritt Island National Wildlife Refuge, Port Canaveral, the Space Coast Stadium, Patrick Air Force Base (PAFB), and Kennedy Space Center (KSC) -- with many parks well suited for viewing space vehicle launches. Any resident or tourist visiting Brevard will find that Brevard County parks offer a rich opportunity for leisure activity and environmental education.
A Sense of Community • Achievement • Respect For Nature • Good Health
Under Florida law, e-mail addresses are public records. If you do not want your
e-mail address released in response to a public-records request, do not send electronic
mail to this entity. Instead, contact this office by phone or in writing.


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Built in 1972 and renovated in 2004, this hotel comprises a total of 28 rooms spread over 3 floors. A garden and terrace additionally feature. Facilities on offer include a foyer with 24-hour reception desk, a hotel safe and lifts. The air-conditioned hotel also comprises a bar, a caf�, a TV room, a restaurant with a smoking area, a breakfast and dining room as well as a conference room and a public Internet terminal with WLAN (for an additional fee). Room and laundry services round up the offerings (for an additional fee). In addition, there is a car park available.
The hotel occupies a 16th century fortress, which was built overlooking Horta Bay and was declared a national monument in 1947. From the hotel guests are able to enjoy breathtaking views of Pico Island as well as the famous harbour. Vulcao dos Capelinhos, Varadouro, Morro de Castelo Branco and Caldera are all just a hour drive from the hotel.
The rooms come with a bathroom with a hairdryer, a direct dial telephone, a radio, a stereo and satellite/ cable TV. Additional fittings include a minibar, a double or king-size bed, centrally regulated air conditioning, a hire safe, carpeting, central heating and a balcony or terrace.
There is a swimming pool and a poolside snack bar available.
Guests may select their breakfast and lunch from varied buffets offered. Lunch and dinner may also be taken � la carte. Special dietary requirements as well as individual-specific dishes may be prepared. Guests are able to book half-board stays.
The following are all accepted as methods of payment at the hotel: Credit card: American Express, Diners Club, MasterCard and VISA, Cash or Deposit.
Room Facilities:
Direct dial telephone
Satellite cable TV
Internet access
Central airconditioned
Central heating


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Nylabone NutriDent Complete 3 Point Bones for dogs are all natural dental chews with added vitamins and minerals. Raised nubs provide 360° cleaning action. Clinically proven to be better than brushing.
Each chew can break apart into 3 smaller chews. Easy to digest with no preservatives or added sugar. Made in the USA.
Size: Small , Filet Mignon Flavor
Quantity: 16 per package
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|Product Description
The Rolling Cargo Bag is big enough to accommodate a comprehensive set of full sailing kit. It also benefits from having wheels for easy transportation.
• Large main compartment with two zipped end pockets
• Two external pockets for quick access to essential items
• Wet and dry compartment keeps the rest of your kit dry
• Detachable shoulder strap with anatomical non-slip padding
• Grab handle either end
• Smooth-rolling non-marking wheels for easy transportation
• L31 X H13 X W14.5 in
Model: L067
* Oversize item
Due to the large size or weight of this item it is considered oversize and does not qualify for free shipping offers. Shipping by freight carrier may be required. Click here for more information.


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Made with 100% pure Merino Wool from New Zealand, the Icebreaker Bodyfit 220 Leggings are close-fitting 'long johns' from Icebreaker's Bodyfit collection.
With Pre-Order, you can purchase the latest items added to our store in advance of them arriving with us.
You Pre-Order is prioritised (at no additional charge); meaning that, as soon as the product arrives with us, we can wrap it straight back up and get it sent out to you - it often never even hits the stock room shelves!
With Pre-Launch, the date when we expect to receive the product ourselves is still a little too hazy to be confident in accepting Pre-Orders.
So, instead, you can submit your email address against the item you are interested in and we will email you as soon as stock arrives on the system.
Estimated Delivery Dates are given on Pre-Order and Pre-Launch products. They are for indication purposes only and can change at any time without notice.
The dates we provide are based on indications given by our suppliers. Whenever we get updated information from our suppliers we will endeavour to update the Estimated Delivery Dates provided on product pages.
Made with 100% pure Merino Wool from New Zealand, the Icebreaker Bodyfit 220 Leggings are close-fitting 'long johns' from Icebreaker's Bodyfit collection. They use 200gm merino fabric for lightweight layering and are perfect for year round active use. Merino wool is a sustainable, ethical and natural fibre that is also naturally odour resistant; a huge benefit over synthetic baselayers that can get notoriously smelly.
The flatlock stitching throughout also ensures that the leggings won't cause chaffing or rubbing when worn under an outer layer.
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WebAdvisor |
Canvas |
Student Email |
Contact Us |
A-Z Index
Del Norte
Eureka Campus
Eureka Downtown
Student Services
Financial Aid
Placement Information
College of the Redwoods is one of ten California community colleges participating in a nationwide Study on Collegiate Financial Wellness, conducted by Dr. Anne McDaniel at Ohio State University. Dr. McDaniel is administering an online survey to a random sample of CR students to gather valuable insights on our students' financial capabilities. This information may provide a pathway to implement support services to not only help students succeed in achieving their education goals, but to provide financial literacy skills that will influence a constructive financial decision-making behavior long after they leave school.
On Monday, February 6, a random selection of CR students received an emailed survey invitation to participate in the Study on Collegiate Financial Wellness.
Please keep an eye out for it and consider completing the survey. We want to learn about your financial experiences during college and how they impact you. This study is a great way for your voice to be heard and to contribute to our understanding of college finances.
Check your student email and fill out your survey today!
Session Length Dates
4 week 05/22/2017 - 06/15/2017
4 week 06/26/2017 - 07/20/2017
8 week 05/22/2017 - 07/13/2017
8 week 06/19/2017 - 08/10/2017
10 week 05/22/2017 - 07/27/2017


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Revamp model is legally sound: International Cricket Council tells Sri Lanka Cricket
Apart from Sri Lanka Cricket, Cricket South Africa and the Pakistan Cricket Board are also unhappy with the proposals that will give a major say to BCCI, ECB and Cricket Australia in the running of the game.
Responding to opposition from Sri Lanka Cricket (SLC) to the proposed changes in the International Cricket Council's structure, ICC's head of legal affairs Iain Higgins has said that the revamp model was "legally sound" and member boards can vote against it should it be brought to vote during tomorrow's Board meeting in Singapore.
On Wednesday, SLC president Jayantha Dharmadasa wrote to Higgins after a special meeting of the SLC stakeholders voted unanimously against the revised proposals presented to the ICC directors at the ICC Board meeting on January 28. (India, England and Australia close to becoming 'Big Three')
According to Cricinfo, Higgins has replied to Dharamdasa, saying that "the proposals themselves could be "considered" within the ICC's "Memorandum and Articles of Association" and members are free to express their opposition.
"On the face of the resolutions themselves, they would appear to be correct. Any Member who disagrees with that analysis or any of the resolutions is, of course, free to vote against the resolutions, or take such other action as they see fit," Higgins wrote.
Apart from SLC, Cricket South Africa and the Pakistan Cricket Board are also unhappy with the proposals that will give a major say to BCCI, ECB and Cricket Australia in the running of the game. (PCB's income could double if they accept ICC revamp)
In his correspondence to Higgins, Dharmadasa had said the "purported proposals" were completely against the fundamental principles on which the ICC had been formed.
He feared that the revised structure would put power in the hands of "just three Full Members" and make available to them a "disproportionately large share" of the ICC funding meant for all ten Full Members. That, he said, would only violate the equal revenue-share model that was part of the ICC constitution. (India deserve lion's share in ICC revenue: Cricket Australia | CSA to not compromise on integrity over ICC revamp plans)
One of Dharmadasa's reservation was against the proposed "contribution costs" which would erase the existing equal distribution of funds to all Full Members from the ICC surplus.
And Higgins admitted that the financial redistribution model might need further discussions.
"Our preliminary view is that the payment of 'Contribution Costs' and 'Test Cricket Fund' are in furtherance of the ICC's objectives and consistent with the prevailing provisions within the ICC's constitution. We will consider that matter further, and will reflect on your comments in that respect," Higgins wrote.
Higgins also said that it was not necessary to give the Members "three months" notice for the proposals to be studied, as argued by Dharmadasa. The period, he said, was necessary only if the resolutions were being tabled in front of the Full Council. The ICC's Full Council meets in June every year.


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Lundberg Brand Rice: Organic Brown Sweet Rice, and Organic White Sushi Rice
Wrapper: Toasted seaweed sheets called Nori
Vegetables: You can add anything here. Just remember to keep pieces sliced thin so you have room for other stuff. Choices include: Baby spinach leaves, Cucumber, lightly steamed carrots, zucchini, squash, sweet potato, green onion, asparagus, etc…
Protein: Have fun with this one. The hot dog choice is kind of goofy, but the kids love it (I do too!) Choices include: Sliced Chicken breast, Uncured Ham or Smoked Turkey breast, Wild Salmon, Organic Chicken, Beef or Turkey Hot dogs, Tuna (either canned
Healthy Fat: Avocado, Hummus, Light Cream Cheese, Lt. Mayo or Soy Mayo, Light Sour Cream, diced nuts (peanuts, almonds etc..)
Embellishments: Gomashio (Toasted Sesame Seeds), Furikake (Japanese Rice Seasoning), various seasonings: cumin, curry powder, chili spice blend, thai spice blend, tumeric, garlic salt etc.


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United States
If you enjoyed my Music Please follow and share my tracks and help me to reach out people all over the world.
Mert Baser is a self-developed DJ/producer from Istanbul,Turkey while studying in Boston, USA.
Music has always been a key aspect of Mert's life and he is just 17 years old, and he started deejaying in 2008. He is obsessed with every kind of music, and he uses professional Pioneer DJ Equipment (CDJ-900, DJM-900).
Feel Free to add comments as feedbacks for me! For Tracks & Remix requests please contact: [email protected]
Twitter: http://twitter.com/#!/DJMertBaser
Press play to try again.
Please update your browser or install another such as Google Chrome.Mixcloud requires HTML5 audio with MP3 and MP4 support.


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It's very hard to choose which shop to feature on Fridays! We have so many talented artists! Each person's skill is unique and what they offer- you can tell they put their hearts and soul into their work!
Today's Featured Shop: JUST BOBBIN ALONG!
Just Bobbin Along is owned and operated by Garilyn which is located in Ohio! Garilyn's work is gorgeous! She makes receiving blankets, 1st Bday Outfits with Tutus, and a host of other items!
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- Return to Fiction by GNeos
Same streets.
Same buildings.
Same predictable people.
…Life is boring...
Everything I watch is becoming monotone and boring. Simple and predictable.
When did the world started to lose meaning?
My name is Raim Hanta: age 17…; personal appeareance…: dishelved black hair (I hate combing it, okay?) and eyes…residen… oh why I am even bothering…
In order words, a normal teenager… with a normal life… which sucks.
I live alone, I didn't know my parents nor I care about who they were or they did, I live by my rules, in order words, I hate being pushed around.
But still, I am part of that endless cycle called daily live. Wake up. Brush your teeth. Dress up. Eat. Go to the high school. Eat again. Practice with a wooden sword (swordmanship is one of the things I respect). Get back home. Eat once more. Sleep. Repeat process again.
I just wanted my life to be have a meaning. Not a life like the rest. Be someone. I almost gave up…..
…Until that event happened. My life did a complete 180º in a blink of an eye.
Human of the other side: A Touhou Project fanfic
Episode 1: Border of Reality and Fantasy
It all started that morning. Like every morning.
- All right, listen up everyone! You too Raim, don't slack off during the day
- Like I care. Hmm?
His name is Mr. Hiran. Your average teacher in your average high school of your average life. No more comments.
- Today we are having two new students in our institude. Be gentle with them. I hope some of you would show some courtesy to them.
- What…you're talking about me?
- Please come in.
The door opened and two girls showed up. The first one had brown hair. She was wearing a black hat and a tie (Why is she wearing it in the first place? This isn't a formal reunion), along with a white shirt and a brown dress. Also, she was holding a book( Is she some kind of book freak?). The other one had blond hair and was wearing a purple vest. What caught my attention was that she was also wearing a white poofyhat on her head. Why she was wearing a hat like that, I`ll never know.
- Hello, I'd like to introduce ourselves. My name is Renko Usami and this is my friend Maribel Han, but just call her Mary.
- Renko! How many times I have to tell you that you should call me by my real name!!
But in the end, they were just normal humans. Heck, I even had wished that they could be aliens or something else, but noooo…
- Okay, enough with the introductions, please have a seat.
And they did. Right next to me (both sides). Joy.
The day continued normally. Writing notes. Memorizing names of "important" people and so on, until Maribel broke the ice.
- Say, what's your name? You haven't spoken any word since the beginning of the day. Are you mute?
- …No. I just don't want to speak.And as for my name, I won't tell you.
- Ehhh?! Why?
- Why? Because I don't want to. Period.
- Hey Mary, leave him alone. It seems he doesn't want to speak . Just let him be.
- But Renko, don't we need someone to guide us in this town?
- Then ask someone else. Not me, thank you.
- You heard the man. Leave him alone. We better ask other person.
- But the others don't seem interested. They think we are crazy. Really, is it a big deal to help us to find the border to that other world.
- Yeah, find the border to…Wait, what? What did you just said?
- Perfect, another person who thinks we are crazy.
- No, no! I'm just …kind of interested.
Their reactions were both a mix of surprise and joy. Really, were they unexpecting a positive answer?
- What?! You don't think we are crazy.
- In fact, yes, but… No, I don't. So you like paranormal stuff? I can't believe I am having a conversation longer than a minute....
- Of course! You see…
And they explained all they knew. I didn't expect a girl like Maribel to be able to watch borders (In fact, I believed that was impossible!) and also to dream about another world with a humanoid nine-tailed fox and a cat (The border part was more believable that this). According to Maribel, that fantastic world is called Gensokyo and is home of many kinds of youkai and also humans.(But don't youkai eat human beings? How they can live in harmony?).
- So let me get this straight, you need my help as a guide of this town in order to find a border or whatever connected to Gensokyo. Great, I
caught the name quickly. And if we find it, to make a report about it, right?
- Yes, you have very good memory.
- Gee thanks.
- So we should start after classes, right Mary?
- Is Maribel, Renko! How many times do I have to tell you?!
And the classes ended. Funny how a little meeting can set course to a different way of living.
That afternoon the three of us went "border seaching". I brought my wooden sword ( gotta be prepared somehow) and some food. I was wearing a green sleeveless sweater, dark blue jeans ( the left part of the jeans was torn so it looks like a combination between jeans and shorts, what else I can say), a red shirt and black sneakers. Maribel and Renko went silent for a moment, but the awkwardness passed very quickly. So we started to find a border in any kind of place, asking people if they had felt something strange in the neighborhood, even using a strange device that, according to Maribel, could pick up disturbances( it looked more like a metal detector and, how did they got something like this? Did they stole it or was hand-made?) to find those borders were the activities of that afternoon.
- Ugh, it's no use. We have searched everywhere in town and there hasn't been a reaction. Does this thing even work?
- Well , we haven't tried using it yet so…
- WHAT?! You're telling me that I have been carrying a piece of junk for nothing.
- Raim, calm down! We just wanted to try…
- And you did! What's the result? Nothing! We have been doing this for how long, four hours? And nothing! I knew this was a bad idea…
- Hey you can't give up just know, maybe something will happen…
- …Maybe. Maybe not. Ok, I won't quit , but if something doesn't happen within the next hour, I'm going ba…
Beep, beep, beep.
- Huh? Could it be…?
I couldn't believe it. The piece of junk actually worked!
- Hey, we have a signal!!
- Really, were?!- Maribel shouted while lifting me with her hands( was she this strong or is it the joy of finally finding a trace of a border I will never know).
- Let's see. What?
- What is it, Rain? - Renko asked.
- It's Raim! And the signal comes from an abandoned shrine!
- What? Where? Why?
- I don't know! Just drop me already! - I begged Maribel and she released me. Now my butt hurts.
- Ouch. Ok, let's get a move on! Quick, follow me, I know where it is!
- Ok, lead the way.
And so we ran. I couldn't believe I could run so fast. Maybe it was the excitement of finding something out of the ordinary or I was this fast already. Anyway, we reached the shrine in less than five minutes. And what we found wasn't exactly what I expected. In front of the shrine…was nothing.
- Huh, that's weird. The signal came from here. What does this mean Maribel?
Silence. Maribel was either not listening to me or spacing out
- Maribel? Hey, earth to Maribel, what's wrong? – Renko asked worried
- …shrine maiden… spell card…danmaku...
- What? What are you talking about? Snap out of it!
I reached my hand to her shoulder to wake her up, but suddenly many things happened in five seconds.
One. There was a tremor. Nobody lost their balance.
Two. Maribel screamed. I could just sworn it was of something being hurt. She pushed me to the stairs we climbed.
Three. While falling I saw something open on thin air. It was some sort of gap with eyes inside of it. I was slowly falling inside.
Four. Renko shouted my name, with an expresion of amusement and terror. Maribel collapsed . The sky filled with many gaps, but then dissapeared. Half of my body was inside the gap.
Five. All my body was inside the gap. The gap closed.
I was between confused and shocked. I found myself floating in a space full of eyes ( do astronauts feel like this at space?) . Thankfully, I could breathe. But all of that didn't matter, as I shouted the three words that could express what I felt.
- Return to Fiction by GNeos


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Hexa Mesh
1 Attachment(s)
I am a new in ansys 13 and I don ´t how to mesh my model with hexa mesh. I want to modelling a combustion in a 3D model. The pictore of my model is atteched. I sliced it for 4 parts, but if I choose AUTOMATIC METHOD ansys 13 always meshed my model with tetra mesh. Please if you know help me.
Thanks a lot :) :cool:
Pick a hex method perhaps (sweep or multizone)?
Yes I tryed a sweep method it create mesh but with the low quality and if I try a Multizone I got this: Multizone blocking decompozition failed. I drawed my geometry in ansys designer.
Define poor quality. Skewness? Which metric are you using? If so is the mesh size too big to represent the geometry? Post a picture to help us understand what you mean by this.
|All times are GMT -4. The time now is 23:16.


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Speaking publicly for the first time since the Boston Red Sox attempted to trade him and signed Adrian Beltre to replace him as the team's starting third baseman, Mike Lowell told WEEI.com he is focusing on getting himself prepared to play baseball and not on his uncertain future in Boston.
"I don't view this as a major challenge," said Lowell, whose offseason thumb surgery derailed his trade to the Texas Rangers. "If I'm healthy and teams out there think I can play every day, there's going to be a team that wants me to play. If that's beneficial to the Red Sox, I don't think any of this is contingent on whether it's beneficial to me. It doesn't matter. I have no say. But if it's beneficial to the Red Sox, then yeah, they'd make the move. And then you'd go wherever they send you. That's the thing. I can't say, 'Hey, I want to leave,' or, 'Hey, I want to stay.'
"It doesn't matter what I think or say. The only time I had that decision was after the World Series when I was a free agent. That's the only time that I had the say. After that, you're a product of the industry. I'm not sour about that. I'm cool with it. If you told me that I'd get traded and then they'd take away my contract, then I might have a much bigger issue with the way things are going."
Red Sox general manager Theo Epstein said earlier this week that Lowell's situation will "take care of itself" and that "Mike's priority is our priority, just to get him healthy, and until that happens, there's not much [to be done]."
Epstein said Lowell will not be ready to swing a bat when position players report and may need a couple of extra weeks as he recovers from thumb surgery. Lowell told WEEI.com he has been encouraged by his recovery and hoped to swing with contact in about a week. Lowell, who is also still recovering from hip surgery he underwent nearly two years ago, said he was healthier going into spring training than he was last season.
Epstein acknowledged that if Lowell showed he was healthy and productive in camp, it might be more likely he gets traded before Opening Day.
"If he's really impressive and impressive to other clubs, then maybe something can be worked out," Epstein said. "If not, I'm sure there's nowhere else Mike would prefer to take a lesser role than here."
Lowell did not rule out the possibility he could end up staying with the Red Sox this season, the last of a three-year, $37.5 million deal he signed after being named MVP of the 2007 World Series.
"Oh, yeah. I think there's a way," Lowell told WEEI.com by phone from his Miami-area home. "What I want and what will happen, I don't know that's something that's attainable. But anything can happen. Guys get hurt all the time. But I don't really want my playing time based upon whether someone gets hurt."


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The Panasonic EY45A2-LJ2G is an 135mm 18 volt circular saw with Dual Voltage capability. So it still works with your 18v and 14.4v batteries. It now comes complete with 2x 5.0Ah batteries for the best run time available!
This cordless circular saw is ideal for a range of applications in metal construction, electric installation work, plumbing, and general construction.
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This IT pilot fish's co-worker is very protective of his mobile phone -- which guarantees that everyone else in the office is looking for an opportunity to make him just a little crazy.
And that opportunity comes one day when he leaves the phone unattended on his desk, and someone just happens to walk off with it.
"He had the theme song from a popular TV series as his ring tone," fish says. "We copied his ring tone onto another phone and taped it under his desk, directly below where he puts his phone.
"We then made sure to present him with his phone back. He did a full check on his phone and couldn't identify anything we had done to it.
"Then, every time he put his phone down on his desk, we rang the other phone.
"By the end of the afternoon he was climbing the walls, trying to work out what we had done to his phone. Obviously he could find nothing, as we had done nothing.
"In the end, we let it ring until he could find the other phone, so he could realize what we had done."
Phone, tablet, PC, terminal -- just tell Sharky your story. Send your true tale of IT life to me at email@example.com. You'll snag a snazzy Shark shirt if I use it. Add your comments below, and read some great old tales in the Sharkives.
Get your daily dose of out-takes from the IT Theater of the Absurd delivered directly to your Inbox. Subscribe now to the Daily Shark Newsletter.


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The World Cup: Why statistics are the best guide to predicting the winners. Our guest is Chris Anderson, author of The Numbers Game: Why Everything You Know About Football is Wrong, and he tells us that too many people, including football club managers, wrongly trust their gut rather than the numbers. Plus BBC correspondents Evan Davies and Katy Watson report on why this is not just a big sporting moment for the World Cup's host country Brazil, it's also showtime for the country's economy. #FullPodcastAvailable #Podcasts
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