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Fluoxetine prevents development of an early stress-related molecular signature in the rat infralimbic medial prefrontal cortex. Implications for depression?
Cellular and molecular mechanisms
Psychological stress, particularly in chronic form, can lead to mood and cognitive dysfunction and is a major risk factor in the development of depressive states. How stress affects the brain to cause psychopathologies is incompletely understood. We sought to characterise potential depression related mechanisms by analysing gene expression and molecular pathways in the infralimbic medial prefrontal cortex (ILmPFC), following a repeated psychological stress paradigm. The ILmPFC is thought to be involved in the processing of emotionally contextual information and in orchestrating the related autonomic responses, and it is one of the brain regions implicated in both stress responses and depression.
Genome-wide microarray analysis of gene expression showed sub-chronic restraint stress resulted predominantly in a reduction in transcripts 24 hours after the last stress episode, with 239 genes significantly decreased, while just 24 genes had increased transcript abundance. Molecular pathway analysis using DAVID identified 8 pathways that were significantly enriched in the differentially expressed gene list, with genes belonging to the brain-derived neurotrophic factor – neurotrophin receptor tyrosine kinase 2 (BDNF-Ntrk2) pathway most enriched. Of the three intracellular signalling pathways that are downstream of Ntrk2, real-time quantitative PCR confirmed that only the PI3K-AKT-GSK3B and MAPK/ERK pathways were affected by sub-chronic stress, with the PLCγ pathway unaffected. Interestingly, chronic antidepressant treatment with the selective serotonin reuptake inhibitor, fluoxetine, prevented the stress-induced Ntrk2 and PI3K pathway changes, but it had no effect on the MAPK/ERK pathway.
These findings indicate that abnormal BDNF-Ntrk2 signalling may manifest at a relatively early time point, and is consistent with a molecular signature of depression developing well before depression-like behaviours occur. Targeting this pathway prophylactically, particularly in depression-susceptible individuals, may be of therapeutic benefit.
Stress is a potent risk factor in the development of mood and anxiety disorders and other psychopathologies. For example, stress is an important non-genetic cause of major depressive disorder (MDD), with both acute and chronic forms capable of precipitating major depressive episodes
To identify as many ILmPFC mechanisms as possible that are involved in the response to repeated stress, we used a genome-wide, gene expression analysis approach. We also chose a restraint stress model as this type of psychological stress affords greater control in application of the stressor than with, for example, the social conflict model. As the neural correlates that underpin the transition to the depressive state are not understood, we tried to identify early stress-induced changes that may increase susceptibility to the development of a full depression-like state. It is known that multiple stress experiences are generally needed to cause MDD in humans and are absolutely required to develop depression-like symptoms in animals, so we used what can be considered a sub-chronic stress paradigm that does not lead to these behavioural symptoms. We found sub-chronic stress resulted in a molecular signature in the ILmPFC, specifically perturbed BDNF-Ntrk2 (note, Ntrk2 is also known as tyrosine kinase receptor type B, TrkB) signalling, that is consistent with known indices of the depressive state and that was prevented with fluoxetine treatment.
Genome-wide gene expression analysis
Illumina RatRef-12 Expression BeadChip microarrays and GenomeStudio software were used for genome-wide screening. As gene expression changes are generally subtle in the brain
Pathways associated with stress-induced gene expression change
To better interpret the stress-induced changes in gene expression in the ILmPFC with regard to potential biological function, the list of differentially expressed genes was subjected to pathways analysis using the Database for Annotation, Visualisation and Integrated Discovery (DAVID)
Real-time quantitative PCR (RT-qPCR) confirmation of stress-induced gene changes
In choosing specific genes for RT-qPCR confirmation, we used one or more of the following criteria: a) presence in enriched gene lists, as determined by DAVID analysis; b) a fold-change in either direction of ≥1.2, as determined by microarray analysis; c) experimental evidence in the literature supporting an involvement for the gene of interest in stress-related mechanisms. For instance, microarray and pathway analyses showed a down-regulation of genes encoding for the neurotrophin receptors
Figure 1
A-D Graphs depicting the effects of sub-chronic restraint stress (RST), fluoxetine treatment without stress (FLX), and fluoxetine treatment with stress (RST+FLX) on gene expression in the ILmPFC
A-D Graphs depicting the effects of sub-chronic restraint stress (RST), fluoxetine treatment without stress (FLX), and fluoxetine treatment with stress (RST+FLX) on gene expression in the ILmPFC. Values are percentage means (±SEM) relative to unhandled controls (dashed horizontal line at 100%). Sub-chronic restraint stress reduced the transcript levels for the plasma membrane neurotrophin receptor genes ntrk2 and ntrk3 (A). Note, chronic fluoxetine treatment prevented the stress-induced ntrk2 but not ntrk3 transcript reduction. Sub-chronic restraint stress reduced transcript levels for the PI3K-AKT1-GSK3B (B) and NTRK2-MAPK/ERK (C) but not the PLCγ1 (D) intracellular signalling pathways. Interestingly, fluoxetine treatment prevented the sub-chronic stress-induced reduction in PI3K-AKT1-GSK3B signalling pathway, but not the NTRK2-MAPK/ERK (B-Raf and MAPK1 genes) pathway. Gene expression of the serine-threonine kinase, mTOR, a downstream target of the PI3K-AKT1 pathway, was reduced by sub-chronic stress, an effect not prevented by fluoxetine (D). * denotes p < 0.05.
The effects of fluoxetine on ILmPFC stress-induced gene changes
As we found significant reduction in BDNF signalling-related genes in the stress group and perturbation in this pathway has been implicated in the aetiology of depression, we determined whether treatment with the antidepressant, fluoxetine, would alter the stress-induced changes in ILmPFC BDNF-related gene expression. As shown in Figure
Stress is a potent risk factor for the development of depression, but the mechanisms that progress the brain's normal response to stress to the pathological state that manifests as depression are poorly understood. Here, we have focussed on the ILmPFC to characterise gene expression changes following repeated, but sub-chronic, episodes of stress. We based our study design on the premise that early neurobiological indices of depression, or at least of the transition into a depression-like state, may be detectable in a sub-chronic model and we chose the PFC because of its known sensitivity to stress and its putative involvement in depression. For instance, stress causes dendritic remodelling in rat IL
To observe depression-like behavioural and other changes in animals, it is necessary for the animal to experience repeated exposure to the stressor over prolonged periods. For example, in a systematic study of the effects of stress episode duration and number of repeats, Kim and Han demonstrated that at least 14 days of 2 hour daily restraint stress were required to produce significant depression-like behaviours
To identify stress-related mechanisms that may potentially lead to the development of pathology, we first carried out a genome-wide gene expression analysis using moderately stringent criteria to define differentially expressed genes. A number of previous studies have also taken this approach
The most significantly enriched pathway, and the one with the lowest FDR, was for neurotrophin signalling. There is a substantial body of evidence implicating BDNF in MDD. For example, BDNF and Ntrk2 transcripts and protein levels were markedly reduced in the PFC and amygdala of MDD subjects
BDNF (or NT3/4) binding to Ntrk2 can activate three main intracellular signalling cascades: mitogen-associated protein kinase (MAPK; also known as the Ras/ERK-MAPK pathway), phosphotidylinositol-3 kinase (PI3K) - Akt, and the phospholipase C-γ1 (PLCγ1) cascades
Interestingly, antidepressant treatment with fluoxetine only prevented the sub-chronic stress-induced changes in the PI3K-Akt pathway related transcripts (PIK3CB and GSK3B genes), with B-RAF and MAPK1 gene transcripts of the MAPK/ERK pathway not significantly different to the stress without fluoxetine levels. This may indicate that the effects of fluoxetine antidepressant treatment are preferentially mediated by the PI3K-Akt pathway, at least in this brain region, and suggest that targeting components of this pathway may be of future therapeutic interest. In this regard it is notable that GSK3B has been implicated in the pathophysiology of mood disorders
We also found sub-chronic stress reduced ILmPFC transcript levels for Ntrk3, the cognate receptor for the neurotrophin, NT-3. Like BDNF, NT-3 has also been implicated in MDD. For instance, NT-3 gene expression was reduced in peripheral blood cells of individuals during depressive but not remissive states
To further elucidate potential neurobiological mechanisms that may increase depression susceptibility, we have used a sub-chronic stress paradigm that is not capable of inducing behaviours that are characteristic of depression, and evaluated the infralimbic prefrontal cortex for molecular indices that may constitute an early signature for this psychopathology. We found that the BDNF-Ntrk2 pathway was affected by the stress paradigm as was GSK3B, which is a component of both the neurotrophin as well as the Wnt signalling cascades. Both the Ntrk2 and Wnt signalling pathways are implicated in depression and, consistent with the notion that our molecular findings represent early depression neuropathology, the stress-induced perturbations in these pathways were prevented by pretreatment with an antidepressant, fluoxetine, a selective serotonin reuptake inhibitor. These findings indicate that brain signalling pathways that are known to be abnormal in fully developed depressive states are actually perturbed well before the manifestation of behaviours that characterise depression.
Adult, male, Sprague–Dawley rats (20–22 weeks of age) were used for all experiments. Animals were obtained from the Animal Services Unit at the University of Newcastle, group-housed (4 per cage) on arrival and maintained in a temperature (21°C ± 1) and humidity controlled environment with food and water available
Experimental procedures
Tissue preparation and RNA extraction
After decapitation and craniotomy, the brain was rapidly removed and cooled in ice-cold diethyl pyrocarbonate (DEPC)-treated PBS. Brains were then placed in an ice-cold metal brain matrix and the frontal lobe separated in the coronal plane and instantly frozen in dry-ice chilled isopentane. Tissue was stored at −80°C until needed. A series of 500 μm-thick coronal cryosections were obtained through the rostrocaudal extent of the ILmPFC (+2.5 to +4.0 mm relative to Bregma)
Gene expression microarray data processing
Microarrays were processed by the Australian Genomics Research Facility (AGRF; Melbourne, Australia). 100ng of total RNA, obtained from the ILmPFC was used for microarray analysis. For microarray analysis we used RNA from 4 of the 6 animals in each group, whereas for qPCR, RNA from each of the 6 animals was used. RNA quality was first checked using an Agilent Bionalyser, and then prepared for hybridization onto Illumina RatRef-12 Expression BeadChips. Arrays were scanned using standard Illumina protocols. RatRef-12 Expression microarrays probe for 21,910 genes. Raw intensity data was then imported into Illumina GenomeStudio Data Analysis Software and statistical analysis of gene expression was carried out using the Gene Expression module (version 1.1.1). First, a background measure based on the average signal of negative control probes was obtained and subtracted from all probes of the array. Next, the data was normalized by the GenomeStudio Average Normalization algorithm to adjust sample signals and minimize variation arising from non-biological factors. The p-values for differential expression were then calculated using the Illumina Custom Error Model algorithm and the Benjamini and Hochberg false discovery rate (FDR), a multiple testing correction method for adjustment of p-values
Real-time quantitative polymerase chain reaction (RT-qPCR)
For confirmation of gene expression changes, RT-qPCR was carried out on mRNA from all 6 animals of each group. Gene-specific primer pairs (Table
Pathway analysis
Pathway analysis of differentially expressed genes was undertaken using the Database for Annotation, Visualization, and Integrated Discovery bioinformatics resource (DAVID;
ILmPFC: Infralimbic medial prefrontal cortex; BDNF: Brain-derived neurotrophic factor; ntrk2: Neurotrophin receptor tyrosine kinase 2; MDD: Major depressive disorder; vmPFC: Ventromedial PFC; FDR: False discovery rate; DAVID: Database for annotation, visualisation, and integrated discovery; KEGG: Kyoto encyclopedia of genes and genomes; LTP: Long-term potentiation; RT-qPCR: Real-time quantitative PCR; mRNA: Messenger RNA; MAPK: Mitogen-associated protein kinase; PI3K: Phosphotidylinositol-3 kinase; PLCγ1: Phospholipase C-γ1; mTOR: Mammalian target of rapamycin; GSK3B: Glycogen synthase kinase 3B; PKA: Protein kinase A; PKC: Protein kinase C; Akt: Also known as protein kinase B (PKB); DEPC: Diethyl pyrocarbonate; RNA: Ribonucleic acid; gDNA: Genomic DNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; AGRF: Australian genomics research facility; cDNA: Complementary DNA; dNTP: Deoxy nucleotide triphosphate; DTT: Dithiothreitol.
Competing interests
The authors declare they have no competing interests.
Authors' contributions
RB carried out all the experimental work, performed the pathways and qPCR statistical analyses, prepared all figures and tables and assisted with the writing of the manuscript. FRW contributed to the conceptual design of the study and assisted with critical revisions of the manuscript. PRD and TAD contributed to data interpretation and critical revisions of the manuscript. DWS was responsible for overall design and execution of the study, analysis and interpretation of the data and writing of the manuscript. All authors read and approved the final manuscript.
This work was supported by the National Health and Medical Research Council of Australia, the Hunter Medical Research Institute, and the Priority Research Centre for Translational Neuroscience and Mental Health at the University of Newcastle. We would like to thank Dr Chris Dayas for his input on the manuscript.
Stress and depression
Life stressors as risk factors in depression
Neural regulation of endocrine and autonomic stress responses
Structural and synaptic plasticity in stress-related disorders
The stressed synapse: the impact of stress and glucocorticoids on glutamate transmission
Corticosteroid receptors and neuroplasticity
The role of BDNF and its receptors in depression and antidepressant drug action: Reactivation of developmental plasticity
Signaling pathways underlying the pathophysiology and treatment of depression: novel mechanisms for rapid-acting agents
Stress, depression, and neuroplasticity: a convergence of mechanisms
Repeated social defeat selectively increases deltaFosB expression and histone H3 acetylation in the infralimbic medial prefrontal cortex
Damage to the prefrontal cortex increases utilitarian moral judgements
Deep brain stimulation for treatment-resistant depression
Deep brain stimulation for treatment-resistant depression: follow-up after 3 to 6 years
Molecular adaptations underlying susceptibility and resistance to social defeat in brain reward regions
Progress in the use of microarray technology to study the neurobiology of disease
Controlling the false discovery rate: a practical and powerful approach to multiple testing
DAVID: database for annotation, visualization, and integrated discovery
Stress-induced dendritic remodeling in the prefrontal cortex is circuit specific
Repeated stress alters dendritic spine morphology in the rat medial prefrontal cortex
Mild, short-term stress alters dendritic morphology in rat medial prefrontal cortex
Structural and functional alterations to rat medial prefrontal cortex following chronic restraint stress and recovery
Stress-induced alterations in prefrontal cortical dendritic morphology predict selective impairments in perceptual attentional set-shifting
Evidence that microglia mediate the neurobiological effects of chronic psychological stress on the medial prefrontal cortex
Two-dimensional assessment of cytoarchitecture in the anterior cingulate cortex in major depressive disorder, bipolar disorder, and schizophrenia: evidence for decreased neuronal somal size and increased neuronal density
Depression-related variation in brain morphology over 3 years: effects of stress?
Subgenual prefrontal cortex abnormalities in mood disorders
Optimization of chronic stress paradigms using anxiety- and depression-like behavioral parameters
The effects of chronic stress on hippocampal morphology and function: an evaluation of chronic restraint paradigms
Essential role of BDNF in the mesolimbic dopamine pathway in social defeat stress
Sustained hippocampal chromatin regulation in a mouse model of depression and antidepressant action
A model of anxious depression: persistence of behavioral pathology
Chronic mild stress (CMS) revisited: consistency and behavioural-neurobiological concordance in the effects of CMS
Neural circuit changes mediating lasting brain and behavioral response to predator stress
Behavioral, neuroendocrine and serotonergic consequences of single social defeat and repeated fluoxetine pretreatment in the Lewis rat strain
Stress duration modulates the spatiotemporal patterns of spine formation in the basolateral amygdala
Chronic restraint stress causes anxiety- and depression-like behaviors, downregulates glucocorticoid receptor expression, and attenuates glutamate release induced by brain-derived neurotrophic factor in the prefrontal cortex
Expression of fibroblast growth factor-2 and brain-derived neurotrophic factor mRNA in the medial prefrontal cortex and hippocampus after uncontrollable or controllable stress
A negative regulator of MAP kinase causes depressive behavior
Corticolimbic transcriptome changes are state-dependent and region-specific in a rodent model of depression and of antidepressant reversal
Stress-induced changes in primate prefrontal profiles of gene expression
Gene regulation in the frontal cortex of rats exposed to the chronic mild stress paradigm, an animal model of human depression
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
Extracting biological meaning from large gene lists with DAVID
Altered gene expression of brain-derived neurotrophic factor and receptor tyrosine kinase B in postmortem brain of suicide subjects
Molecular evidence for BDNF- and GABA-related dysfunctions in the amygdala of female subjects with major depression
Serum and plasma BDNF levels in major depression: a replication study and meta-analyses
DNA methylation profiles of the brain-derived neurotrophic factor (BDNF) gene as a potent diagnostic biomarker in major depression
Postnatal repeated maternal deprivation produces age-dependent changes of brain-derived neurotrophic factor expression in selected rat brain regions
Brain-derived neurotrophic factor and hypothalamic-pituitary-adrenal axis adaptation processes in a depressive-like state induced by chronic restraint stress
Chronic antidepressant administration alleviates frontal and hippocampal BDNF deficits in CUMS rat
Anterograde transport of brain-derived neurotrophic factor and its role in the brain
Stress and antidepressants differentially regulate neurotrophin 3 mRNA expression in the locus coeruleus
Early maternal deprivation reduces the expression of BDNF and NMDA receptor subunits in rat hippocampus
Rapid induction of BDNF expression in the hippocampus during immobilization stress challenge in adult rats
Immobilization stress rapidly modulates BDNF mRNA expression in the hypothalamus of adult male rats
Trk receptors: roles in neuronal signal transduction
TrkB signalling pathways in LTP and learning
Altered synapse formation in the adult somatosensory cortex of brain-derived neurotrophic factor heterozygote mice
Essential role for TrkB receptors in hippocampus-mediated learning
Reduced presynaptic efficiency of excitatory synaptic transmission impairs LTP in the visual cortex of BDNF-heterozygous mice
Nature vs. nurture: can enrichment rescue the behavioural phenotype of BDNF heterozygous mice?
Regulation of the mTOR complex 1 pathway by nutrients, growth factors, and stress
mTOR signaling: at the crossroads of plasticity, memory and disease
Glycogen synthase kinase-3 in the etiology and treatment of mood disorders
Inhibition of glycogen synthase kinase-3 is necessary for the rapid antidepressant effect of ketamine in mice
Alteration in kinase activity but not in protein levels of protein kinase B and glycogen synthase kinase-3beta in ventral prefrontal cortex of depressed suicide victims
In vivo regulation of glycogen synthase kinase-3beta (GSK3beta) by serotonergic activity in mouse brain
A novel role of the WNT-dishevelled-GSK3beta signaling cascade in the mouse nucleus accumbens in a social defeat model of depression
The glamour and gloom of glycogen synthase kinase-3
Wnt2 expression and signaling is increased by different classes of antidepressant treatments
Altered expression of neurotrophic factors in patients with major depression
Neurotrophin receptor activation and expression in human postmortem brain: effect of suicide
Neurotrophin levels in postmortem brains of suicide victims and the effects of antemortem diagnosis and psychotropic drugs
Increased cerebrospinal fluid levels of neurotrophin 3 (NT-3) in elderly patients with major depression
Bi-phasic change in BDNF gene expression following antidepressant drug treatment
Animal models of neuropsychiatric disorders
Fluoxetine reverts chronic restraint stress-induced depression-like behaviour and increases neuropeptide Y and galanin expression in mice
Long term treatment of depression with selective serotonin reuptake inhibitors and newer antidepressants
Effect of chronic fluoxetine treatment on male and female rat erythrocyte and prefrontal cortex fatty acid composition
Chronic fluoxetine treatment partly attenuates the long-term anxiety and depressive symptoms induced by MDMA ('Ecstasy') in rats
Improved RNA preservation for immunolabeling and laser microdissection
Analyzing real-time PCR data by the comparative C(T) method


text:
The Japanese Civil War, known in Japanese as the Boshin War, was the last civil war in Japan before the collapse of the feudal system. Seeds for the war were planted when the Shogun Tokugawa Ienari announced plans to modernise and industrialise Japan with the help of western nations. Many daimyo were against this, and formed a 'reactionary coalition' known as the Domain Alliance to establish a new isolationist shogunate under Shizamu Narioko. After Shizamu was declared Shogun by the Domains, Tokugawa declared war on the Alliance, beginning the civil war.
In early 1808, an American convoy carrying supplies and military advisors to Edō was attacked by the Domain Alliance, creating a pretext for direct American intervention in the war. With American and limited Spanish support, the Tokugawa shogunate toppled the Alliance and restored rule from Edō in the rebelling Domains.
Currently, a peace treaty is being negotiated in Sapporo.

subset: commoncrawl


text:
Written especially for young children, these classic New Testament stories introduce children to the Bible in a fun way! Each captivating story emphasizes a lesson so parents and children can discuss life applications. Easy to understand text and simple truths make this collection perfect for gift giving, Sunday School lessons and everyday learning at home.
Includes a 26 Page Beautifully Illustrated PDF for little ones to follow along with, containing: 12 New Testament Bible Stories, Scripture References for each story, and 12 Life Application Lessons geared for young children. When you are done reading, sing along with 5 songs of faith!
Download the accompanying reference guide.
©2012 Twin Sisters IP, LLC (P)2012 Twin Sisters IP, LLC
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Can't Post links with a thumbnail to my Facebook page
I am having a problem that similar to others in the forums with not being able to post a link to Facebook. The problem is ONLY for links to sub-pages on my website which is relevancepath.com). I can post links with thumbnails to other wordpress websites.
In addition, I AM able to post a link to the home page but that's it. Links to sub-pages (eg. relevancepath.com/blog) give an error message in Facebook as "Oops. Something went wrong. We're working on getting it fixed as soon as we can." BTW, it makes the thumbnail and the link preview correctly, it just won't let me post. All I get is the "oops we're fixin' it" message.
NOTE: there is a topic on FB's discussion board for this, but as you can see it is unresolved.
A colleague has tried to post a link from our website to her facebook page and she can't either. So the problem is not just restricted to my facebook account.
I'm running WordPress for my site using the Thesis theme. To troubleshoot I have tried changing the theme, disabled all plug-ins and had my business partner try and post to facebook.com/relevancepath (he is an admin also). Nothing.
Having a business fan page will be a waste if I can't update it with info from our website! Can anyone help? Thanks in advance.
- The topic 'Can't Post links with a thumbnail to my Facebook page' is closed to new replies.


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1950 Chevrolet Fleetline Deluxe 216 I6 3 spd manual 2 door all original
Price: US $2,940.00 Item location: Rantoul, Kansas, United States
- Condition: Used
- Make: Chevrolet
- Model: Fleetline Deluxe
- Type: Coupe
- Trim: Standard
- Year: 1950
- Mileage: 81,834
- Color: Black
- Engine size: 216 I6
- Number of cylinders: 6
- Fuel: Gasoline
- Transmission: Manual
- Drive type: RWD
- Interior color: Black
- Vehicle Title: Clear Want to buy? Contact seller!
1950 Chevrolet Fleetline DeluxeThis auction is for a black1950 Chevrolet Deluxe Fleetline 2-door sedan with the optional fender skirts. This car is all original and has been in my family for over 30 years. I am the 3rd owner. Car has 81, 834 original miles and still has the original spare tire and tool kit in the original cardboard box. Car runs and drives great! Issues with the car are that the radio doesn't work (possible issue with the tubes), the windshield wipers don't work (vacuum jar is broken), paint on fenders is peeling, some dings in the side chrome, and the upholstery is original so there's wear on the seats and backs. Car has been garaged all of it's life.
Please ask any questions you might have before bidding. I reserve the right to cancel this auction early as long as there are no bids, as I have the car for sale locally. I require a $500.00 deposit through PayPal within 48 hours of auctions end, and the remainder in cash or through PayPal within 7 days of auctions end. I realize you have many choices on Ebay and I want to say thank you in advance for taking your time to look at my auction. I wish you the best in all your Ebay bidding!!


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$ 12.00
A set of 12 cellophane bags with custom toppers featuring our colorful stripes from the neverland pirates collection, our pirate ship silhouette and can be personalized with your child's name. These bags (3.75" in width) are perfect to fill up with candy or crayons as a party favor, and coordinate perfectly with our neverland pirates collection. Please provide your text requests below.


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3 females 1 male born 5/9/17 $300 Dad (blue nose pit) and mom (red nose pit) on site. $100 non refundable deposit will have contract stating u put…$300
Tiny and beautifully marked tri-color male puppy for sale. ACA registered, well socialized with children and adults. Both parents under 15 pounds. Will…$1,200
Full AKC, intact. Beautiful color, coat, head and ear! Hard to find a nice looking Cav like this. Sweet and loving, loves EVERYONE$2,250
Cream with white markings. Full AKC. Can ship for additional cost. Charting to be approximately 5lbs. 6 month genetic guarantee, vet checked, will have…$750
We have 2 girls left in this litter that do not have deposits. Full AKC, both black with white markings. Dam is 120lb black with white (does have heart…$1,500
Cedar is a playful fawn colored puppy with a black mask. He has lots of personality, he is well socialized with kids and will make a wonderful pet. However…$500
Sequoia is an adorable fawn colored puppy with a black mask. She has lots of personality is well socialized with people and will make a wonderful pet.…$600
1 black and white parti male Will have first shots dewormed vet checked ACA registered Born 2-8-17 will be ready 4-8-17 Can't get pics to download…$500


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...taken away from your family, just because you tried to make a living and a loving home for them. My prayers go out to this family. God bless you and your friends and co-workers. I hope this family will reunite soon. Annette M. Osborne Juneau
...they go by each day. Thanks again for all the work you put into your decorating. It's so enjoyable. Annette M. Osborne Juneau ... for The Glory Hole's special holiday season On behalf of patrons of The Glory Hole, I would like publicly...
...great joy in my heart. Once again, thank you. And I hope that Tlingit-Haida Central Council will have this program to offer us again. I am sure that many people will join this again. A heart-filled thank you. Annette M. Osborne Juneau
...before to help with the decorations. Alright, to you Christmas snatcher, be careful. We are watching out for you. And to my Auntie and Uncle, keep up the beautiful work. I am looking forward to Valentine's Day. Annette M. Osborne Juneau
Juneau Empire ©2013. All Rights Reserved.


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|Angela Schreiber <anch...@day.com>
|Re: Contents of jcr-server ?
|Tue, 07 Nov 2006 07:53:39 GMT
hi cédric > About the 'jcr-remoting-over-webdav' part, I understant that the > JCRWebdavServer stands as the server side, but do you know if someone > ever worked on the JCR client part, to build the whole chain : > JCR API -> WebDav request -> network -> JCRWebdavServer -> JCR Repository yes -> take a look at the spi-contrib... we ended up defining a separate layer (SPI). you may find further info as well as a summary of open issues within /contrib/spi/ and last but not least david recently startet a thread regarding the spi in general: http://thread.gmane.org/gmane.comp.apache.jackrabbit.devel/8674/focus=8728 regards angela
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Mr. PITTS. Mr. Speaker, in March, to mark the 2-year anniversary of the signing of ObamaCare, I invited local doctors, business owners, and elected officials to talk about how the law will change the practice of health care on every level.
We heard from Dr. Gerald Rothacker about the law's failure to reform medical liability and how young doctors don't even realize they're practicing defensive medicine, ordering unnecessary tests, and driving up costs. We heard from Gary Alexander, Pennsylvania's secretary for the Department of Public Welfare, about how the law will
cause State spending to explode in the coming years.
While Medicaid consumes 30 percent of the State budget now, if the law is fully implemented, it will consume over 60 percent. Pennsylvania won't be a State government. It will be a health plan that paves roads and funds schools on the side.
We also heard from Kirby Sensenig, the owner of a local roofing company. He spoke about the difficult choice he will have to make in coming years about whether to keep providing insurance for his workers, or to pay the penalty and cast them into the health care exchanges.
We all know how much health care reform was needed. We've all heard the stories about individuals denied care that they desperately needed. We've heard about the families who struggled to pay the bills when a child got sick. We all know someone who went without insurance because of the high cost of premiums. But this law did not fix what was broken. It didn't deliver the reform the American people really wanted.
The savings, both for the government and for families, are an illusion. Don't pat yourselves on the back for gaming the CBO. There won't be a single dime saved by the law. Indeed, government spending will explode because of the law. ObamaCare's new entitlement program and massive Medicaid expansion is estimated to cost taxpayers $1.8 trillion over the next decade.
Things aren't much better for families under ObamaCare. In 2011 alone, the annual premium for an employer-sponsored family plan soared past $15,000. That's a sharp 9 percent increase from 2010. Twenty new taxes impose additional costs on everything from flexible spending accounts to pacemakers. That's 20 new taxes in ObamaCare not yet implemented. It will be taxing medical devices, drug manufacturers, and insurers. It drives up the cost of care. It is a farce to think that government subsidies will balance out the increased costs imposed by all the new taxes and regulations.
Finally, the reach of the Federal Government has extended even to the conscience of religious charities and educational institutions. When the government takes over health care, it takes over basic decisions of morality that should be left up to individuals. It's far past time we ended the destruction of our health care system by a poorly designed and administered law. Full repeal is the only way to get to real reform. I urge support for the bill.
Mr. PITTS. If this new law is so outstanding, I wonder why the administration has granted over 1,000 waivers to their friends so they don't have to meet the requirements of the law.
The President promised not to raise taxes on anyone making less than $250,000. He has broken that pledge 20 times with this new law. Many of them are impacting the middle class that he promised not to raise taxes on. That's $800 billion in new taxes.
My friends, the American people have a clear choice: Keep this law and pay the new taxes or take the law off the books and let's start over again with some real free-market reforms.
I urge support for the bill, and I yield back the balance of my time.
Source: http://thomas.loc.gov


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Henge is a non-canon character that makes an appearance in the Mortal Kombat comic book, Battlewave. He comes from Nightmare Realm called by the ninjas that were defeated by Liu Kang and Bo. Henge was defeated by Liu Kang after having a short fight with Bo.
Although he presented himself as a strong warrior, with a confident look, Liu Kang easily took him out with a simple kick to his face.


text: Shortlists for WBA 2015 Wi-Fi Industry Awards announced28 Sep 2015 03:25 PM Wireless Broadband Alliance Wi-Fi Industry Awards features 12 awards with the winners dues to be revealed on 14 OctoberThe Wireless Broadband Alliance has unveiled the shortlists for the WBA 2015 Wi-Fi Industry Awards 2015. The 10 Awards, across 5 categories, have been judged independently of the WBA by a panel of analysts and industry experts led by Joe Madden of Mobile Experts.Two additional awards, The Chairman Awards, are voted for by WBA members and recognise the impactful influences that have been driving key initiatives in the Wi-Fi ecosystem over the past 12 months. The winners will be announced at a gala awards dinner and networking event at the Wi-Fi Global Congress in San Jose taking place on 14 October 2015. Joe Madden from Mobile Experts, said: "Increasing confidence in the Wi-Fi ecosystem has driven unprecedented acceleration in deployments over the past 12 months as the global ecosystem moves forward with the adoption of next generation Wi-Fi. This in turn has led to an expanded role of Wi-Fi in services, network technologies, management solutions, devices, applications and new ventures."As judges, we were faced with the difficult task of creating a shortlist from an overwhelmingly high standard of entries. I look forward to congratulating the winners at the awards ceremony in San Jose." The shortlist for the WBA 2015 Wi-Fi Industry Awards is as follows: Best Wi-Fi Service• Best Wi-Fi Network Operatoro Aptilo Networks & TeliaSonera – Best Wi-Fi Serviceo Boingo Wireless – Boingo and Sprint Make Carrier Offload a Realityo KT – KT GiGAtopia Life with GiGA Wi-Fi• Best High Density Wi-Fi Deployment in a Venueo BT – Glh Launches Free High Density Wi-Fi to Differentiate London Hotel Estateo KT – KT Wiz Parko Ruckus Wireless – Ruckus Wireless for the Palace Hotel, WBA Wi-Fi Conference 2015• Best Wi-Fi Deployment in a City or Public Areao MaximaTelecom & JSC – Moscow Metro Public Wi-Fi (City of Moscow)o Ruckus Wireless – A Smarter City in San Jose with Wickedly Fast Wi-Fio Shaw – City of Calgary and Shaw Partnership Best Wi-Fi Network Technology• Best Wi-Fi Network Infrastructureo KT – GiGA Wi-Fi Homeo SK Telecom – Virtualization of Wi-Fi Management Solution by SK Telecomo Time Warner Cable – Innovative Deployment for Environmental Challenges• Best Wi-Fi Service Innovationo Cisco – Cisco Mobility IQ: Unlock the Power of Visual Network Knowledgeo KT – KT GiGA LTEo Ozone – SpeedFetch – Disruptive Downloading for Low Cost & Emerging Markets Best Wi-Fi Service or Management Solution• Best Wi-Fi Management Solution for a Service Providero BandwidthX – BxMarket – Unlocking the Market Value in Dark Wi-Fio Smith Micro – SmithMicro Software – NetWiseo Tutela Technologies – Wi-Fi Management via Device Crowdsourcing• Best Wi-Fi Service Solution for Consumers or Enterpriseso ADTRAN – ProCloud Portfolioo Cisco – Cisco Hyperlocation for Pinpoint Accuracyo Cloud4Wi – Redefining Brick and Mortar Wi-Fi Solutions Best Wi-Fi Device / Application• Best Wi-Fi Enabled Consumer Electronics Device and /or Serviceo Ruckus Wireless – Causing a Ruckus in San Jose and San Francisco with Hotspot 2.0 Release 2o Samsung Electronics & SK Telecom – LTE Wi-Fi Aggregation Serviceo World Media and Technology Corp – Space Lumina• Best Wi-Fi Enabled Device and/or Service for Vertical Marketso Boingo Wireless – Innovating for the Armed Forces: Boingo Broadband and IPTVo Cisco – Cisco Industrial Wireless 3702: Extending Wireless Network Connectivity for IoTo Ruckus Wireless – Ruckus Redefines High Speed, High Capacity Wi-Fi with Industry's First 802.11ac Wave 2 Access Point Best New Ventureo MaximaTelecom – MaximaTelecom Specializes in Establishing Complex Communication and IT Processes and Systemso Tutela – Wi-Fi Management via Device Crowdsourcingo Veniam – Veniam and the Internet of Moving Things Chairman Award – Operator Categoryo AT&To Boingo Wirelesso BTo Fono Orange Chairman Award – Technology Partner Categoryo Accuris Networkso BSG Wirelesso CableLabso Huawei Technologieso Ruckus Wireless James Atkinson EditorLinkedIn |Google + |Email Leave a CommentPlease write code to prove you're humanMore on... Wi-Fi GeneralXirrus Wi-Fi deployed in London's St Pancras StationQualcomm unveils next gen 802.11ax Wi-Fi portfolioRuckus and Wifirst to provide Wi-Fi solution to hotels and campsitesMore on... Smart CitiesNokia unveils wireless router for power distribution networks ETSI forms new industry group for smart city solutionsMore on... Ruckus WirelessRuckus and Wifirst to provide Wi-Fi solution to hotels and campsitesRuckus Wi-Fi combines with TrackR to boost asset tracking capabilitiesArmed forcesFinnish Defence Forces to deploy Bittium Tough Mobile smartphonesFinnish Defence Forces demonstrate ESSOR high data rate waveform Thales unveils two whitepapers on military radio communicationsLatest in Wi-FiXirrus Wi-Fi deployed in London's St Pancras StationQualcomm unveils next gen 802.11ax Wi-Fi portfolioRuckus and Wifirst to provide Wi-Fi solution to hotels and campsites


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About Gothenburg
Friendly, relaxed Gothenburg may be Sweden's second largest city after the capital, but for many this is by far the better city to visit, or to live in. The neo-classical architecture lining the streets, busy with students and trams, has plenty of charm, but there's also plenty of fresh art and architecture brewing here, in the buzzing hipster cafes or live music venues. The best places to stay in Gothenburg come in all shapes and sizes and to suit all budgets - this is, after all, the home of Scandinavia's largest university, and there's plenty of very affordable fun to be had here.
Gothenburg's waterfront is working, but also historic - this was once Sweden's only western port and the centre of Swedish ship building - so you'll find some ships bringing in the freshest fish, while some bob by the quay as museum pieces.
Behind that you'll find a few scenic,17th Century canals, the dressy Avenyn boulevard with its high end boutiques and graceful buildings, then the creative and fashion districts.
Gothenburg's highlights include a number of really wonderful museums. The themes range from your standard modern art museum to the 'Universeum' - Sweden's great kid's museum, to the random vintage treasures of the Mölndals Warehouse Museum and the Maritiman, the world's largest floating ship museum.
You'll also want to visit the 17th Century hilltop fort of Skansen Kronan and the local Haga district, which has pretty 19th Century wooden houses. And possibly Liseberg, Scandinavia's largest amusement park.
Foodies, or those in search of a tasty bargain, won't want to miss the fish market.
Our recommendations for the best places to stay in Gothenburg are both centrally located, so all the city's highlighs are easily accessible.
For central location
- The Elite Plaza Hotel is a large four-star hotel with a gym, saunas, two restaurants and an English style pub. It's great for comfort, and value, and it's by the river and close to many sights.
For five star luxury
- Add a star and you've got the Avalon Hotel a feng shui wonder just a few blocks away. As well as a sense of calm you'll get a roof terrace, pool, bar, restaurant and free wi-fi.
Still not sure which to book? Browse through our list of all the best places to stay in Gothenburg, or contact one of our friendly Gurus for advice.
Best Hotels in Gothenburg
Hotel Flora, Gothenburg 68 rooms from $85Gothenburg
Situated in the centre of Gothenburg, a boutique hotel with interesting design and fantastic work spaces. Flora offers guests a trendy lobby, cosy terrace offering food and drink and unique spaces for meetings and showcases. Rooms are full of character and perfect for exploring this eclectic city.Great walks - Explore Botaniska - local botanical gardensSights nearby - Trädgårdsföreningen - a local palm houseLively area - In the thick of the action.
Elite Plaza Hotel 127 rooms from $117Gothenburg (Box 11065)
Perfectly located five star property in central Gothenburg. The hotel offers a charming restaurant, an English style pub and sultry Plaza bar. Bedrooms are well-equipped with comfortable beds, satellite televisions, toiletries and free WiFi. Cultural sites nearby.Swimming Pool - Indoor/outdoorSights nearby - Well located for major sights.
Hotel Villan 26 rooms from $137Gothenburg
Located in the Eriksberg district - a 10 minute ferry ride from Central Gothenburg. Hotel Villan is a refurbished traditional wooden building overlooking the harbour. Each of the bedrooms has underfloor heating, comfortable beds, all are elegantly decorated. Try local fish in the hotel restaurant.Designer Shopping - Röhsska Museum - a fashion hub and museumKayaking - Gothenburg Archipelago ExplorationSights nearby - Slottsskogen - a large local parkGolf - Jörgen Golfklubb
Avalon Hotel, Gothenburg 98 rooms from $186Gothenburg
Five-star Gothenburg hotel which is centrally located and well-equipped. The hotel has been designed in accordance with the principles of feng shui, providing an ambience of calm. Also available is a roof terrace, swimming pool, restaurant, bar and free WiFi. Perfect location for business or leisureDesigner - Uniquely designed interiors.Swimming Pool - Indoor/outdoorSights nearby - Well located for major sights.
Dorsia Hotel 37 rooms from $253Gothenburg
This modern, boutique hotel is a vibrant and exciting gem just minutes form Gothenburg Central Station. Bold guest rooms are opulent in design and come with modern luxuries. The hotel restaurant serves international cuisine throughout the day as well as a welcoming afternoon tea.Sights nearby - Trädgårdsföreningen Palmhuset just a short walk away - Gardens and palm houseKayaking - Canoe the Gothenburg archipelagoRestrictions on children - Children Over 10 onlyFull of character
The Upper House, Gothenburg 49 rooms from $332Gothenburg
Located at the top of Gothia Towers, this luxury hotel offers extensive penthouse suites, modern elegant bedrooms and a glass bottom swimming pool. Upper house boasts a large spa and gym complex as well as a modern cuisine restaurant with stunning views just 10 minutes from the city center.Learning - Step inside one of Gothenburg's many museumsOther Activities - Enjoy a gig in Sweden's foremost music cityDesigner Shopping - Many designer shopping districts nearbyClose to nightlife - Explore the main boulevard Avenyn
Hotel Pigalle 38 rooms from $393Gothenburg
The design of this luxury hotel in the heart of Göteborg is inspired by the sinful Paris of the early 1900's. A decadent bar, restaurant with charming terrace and sultry bedrooms - this is a hotel to fall in love with. Try delicious local seafood dishes and original drinks in Bar Amuse.Kayaking - Gothenburg archipelago, the city canals or the calm inland lakes.Sights nearby - Visit the old City Hall dating from 1654Local markets - Feskekôrka - the local fish marketOther Activities - Visit Gothenburg archipelago


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Check Point
: 30 October, 2008
(Company News)
Deliveries of Checkpoint Endpoint Security and Network Access Control products make high contribution to quarter earnings
Check Point Software Technologies has announced revenue of $199.7 million for the quarter ended September 30, 2008. GAAP net income and diluted earnings per share for the quarter were $80.1 million and $0.37, respectively. Non GAAP net income and diluted earnings per share for the quarter were $94.2 million and $0.44, respectively.
The regional revenue numbers for the Third Quarter of 2008 are:
42% Americas
13% APAC region and Japan
45% Europe and Middle East
"We are very pleased with our performance during the quarter and believe it can be attributed to the continued implementation of our total security strategy." said Gil Shwed, chairman and chief executive officer at Check Point. "During the quarter we added many new customer accounts across all industries despite the challenging economic environment. As a result of our successful execution we achieved financial results at the upper range of our guidance for both revenues and earnings per share."
Financial Highlights for the Third Quarter of 2008:
* Total Revenues: $199.7 million compared to $184.0 million in the third quarter of 2007.
* GAAP Net Income: $80.1 million or $0.37 per diluted share as compared to $76.7 million or $0.34 per diluted share in the third quarter of 2007.
* Non GAAP Net Income: $94.2 million or $0.44 per diluted share compared to $91.9 million or $0.41 per diluted share in the third quarter of 2007.
* Operating Income: GAAP operating income was $90.4 million, compared to GAAP operating income of $77.7 million in the third quarter of 2007. Non-GAAP operating income was $106.2 million, or 53% of revenues, compared to non-GAAP operating income of $96.3 million, or 52% of revenues, in the third quarter of 2007.
* Deferred Revenues: As of September 30, 2008, we had deferred revenues of $272.9 million, an increase of $45.7 million or 20% compared to deferred revenues as of September 30, 2007.
* Operating Cash Flow: $115.1 million in cash flow from operations compared to $88.8 million in the third quarter of 2007. During the third quarter we received $22.5 million refund from the Israeli tax authorities.
* Cash: As of September 30, 2008, we had $1.4 billion in cash and cash equivalents, deposits and marketable securities.
* Share Repurchase Program: During the third quarter of 2008, Check Point repurchased approximately 2.0 million shares at an approximate cost of $49.8 million.
Business Highlights for the Third Quarter of 2008:
Our total security strategy was extended this quarter with the general availability of Check Point Endpoint Security, the first single agent for PCs to combine the highest-rated firewall, network access control (NAC), program control, remote access, antivirus, anti-spyware, full disk
and media
with port protection. By creating a single endpoint security agent with a single client interface we dramatically simplified endpoint deployment and management for our customers. Our endpoint solution was also recognized as a leader in Gartner's Magic Quadrant for Mobile Data Protection during the quarter.
We continued to offer open choice for deployment of our network security solutions and introduced VPN-1 Virtual Edition (VE), making Check Point the first security vendor to provide unified security management for both physical networks and virtual applications. Check Point's VPN-1 VE restores the separation and security of applications on virtualized servers.
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Posted: Tuesday, December 20, 2016 12:45 PM
I am looking for a sexy lady who is looking to have some fun, safe, unadulterated, play time. I am looking for someone who enjoys anal, both ways, as much as I do. I am as straight as straight can be and its sad I have to say that. Because a guy likes anal play he must be gay......uh, no. You have to like the same sex to be gay. Anyway, looking for a hot lady with a big strap on that wants to have a blast. I would be very generous and appreciative. 420 friendly. Please be genuine and have a pic or 2. I'm white, 6'2", 200lbs, very generous.
• Location: BG/Nashville, Bowling Green
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Fedora 13 is Live
The latest release features improved device access, improved virtualization.
Red Hat Inc. announced the release of Fedora 13, the Linux distro and with it comes a list of new features that enhance performance, access and quality assurance.
Fedora 13 contains an improved installer interface that handles partitioning, driver installation, and storage devices more efficiently. Packaged software will allow for scheduled backups, automatic photo import and management.
Fedora 13 also gets improved driver support in the form of automatic printer driver installs and improved 3D graphics drivers for Intel, Nvidia and ATI video cards.
Kernel-based Virtual Machine (KVM) is now more efficient, as is performance on large-scale multiprocessor systems. Its now possible to for virtual machines to use the same physical network interface cards as the host OS.
Mixed library debugging within GNU debugger (gdb) is now more comprehensive and the SystemTap utility now supports static probes, which allow application developers to abserve how their code interacts with system resources. SystemTap also includes scripts that show Python activity across the entire system and monitor Python call and return functions. These tools make it easier to identify choke points that occur between an application and its function calls. Both Python 2.6 and Python 3 are included in Fedora 13.
On the filesystem front, Fedora supports Btrfs, with added support to filesystem snapshots. Snapshots allow the system to be reverted to a previous state.
Fedora 13 is available now here.
Issue 201/2017
Buy this issue as a PDF
Kolab Now Integrates Collabora Online
Kolab Now subscribers now have access to Collabora Online.
Endless OS, a Distribution Without Internet
A new version of Endless OS has been released.
GitHub Announces Open Source Friday Program
They encourage people to take time out and work on open source projects on Friday.
System76 Announces Their Own Distro, Pop!_OS
Pop!_OS is based on Ubuntu and uses the Gnome stack.
Linus Torvalds Talks About His Motivation
He never tires of working on the Linux kernel.
Debian 9 Stretches Its Wings
The new release of Debian has a very strong focus on security.
Trojan Turns Raspberry Pi into a Cryptocurrency Mining Device
Two trojans in the wild are targeting Linux machines.
Fedora 26 Beta Comes with New Features
The workstation comes with the latest Gnome desktop environment and support for many application build systems.
Raspberry Pi Foundation Merges with CoderDojo Foundation
The two foundations join forces to expand their efforts to reach young people.
Samba Security Hole Patched but Risk is Bigger
Millions of devices that use Samba Server are vulnerable to attacks.


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LONDON (AP) The global economic turmoil may be forcing shops to slash prices, but European football clubs won't expect be expecting similar winter bargains when the January transfer window opens Thursday.
Even West Ham, with its owner facing bankruptcy due to the collapse of Iceland's banking sector, says it won't be selling its best talent on the cheap.
Likewise, even though Manchester City is now bankrolled by wealthy Arab investors, manager Mark Hughes has said he won't be embarking on an unchecked spending spree — despite being linked with many of the world's best players from Kaka to Lionel Messi.
City broke the British transfer record to bring in $50 million Brazil striker Robinho on the last day of the September transfer window as Abu Dhabi United were finalizing its purchase of the underachieving club.
"We're aware of this assumption about the level of finance that we'll throw at the project in January, but we still have realistic market values," Hughes said Wednesday. "There is also an assumption that we will stimulate the market, that the money sloshing around will be from the deals that we have started, but we have to make sure we protect the club as well."
City's transfer ambitions are likely to be modest in this transfer window, as many of the world's best players are committed to teams competing in the Champions League and are unlikely to want to move midseason to a club only two points above the relegation zone.
"We know that there will be a premium that we will probably have to pay because it's January, and teams don't want to lose their better players," Hughes said. "But it will not be to the extent where we are paying hugely over the market value."
Buying from the continent will cost more than 12 months ago with the pound losing 30% of its value against the euro.
For instance Portsmouth's deal to sign Algeria defender Nadir Belhadj from Lens for euro4.5 million on Tuesday would have equated to 3.3 million pounds last January, but is a million pounds more now.
Some Pompey players, including Jermain Defoe and Glen Johnson, are being linked with moves to Tottenham to be reunited with manager Harry Redknapp.
"If the price is right and (Portsouth) want to sell (Defoe) and it's what we consider at our valuation and theirs, then fine.," Redknapp said Wednesday. "There are other options as well, I have a little list and if we don't get one we can move to another."
A Tottenham bid for West Ham striker Craig Bellamy has already been turned down.
Spurs replaced Juande Ramos with Redknapp in October to arrest their decline, but are only out of the drop zone on goal difference.
Ramos has since taken charge at Real Madrid until to the end of the season and has been linked with some of his former White Hart Lane charges to help the defending champions close the 12-point gap on leader Barcelona.
Upton Park isn't going to be the bargain basement for talent that some clubs might have expected. Although beleaguered owner Bjorgolfur Gudmundsson is looking for a buyer for the club, he knows that to retain its value it must maintain its top-flight status.
"We've been assured as senior players that there won't be a fire sale," West Ham goalkeeper Robert Green said. "But it's something completely out of our control at the moment."
Liverpool manager Rafa Benitez will likely have to sell players if he wants to add another striker to his league-leading squad, and winger Jermaine Pennant has been linked with Real Madrid.
Luiz Felipe Scolari believes he needs another striker to close the three-point gap on Liverpool, but chief executive Peter Kenyon has repeatedly ruled out any purchases.
Defending champion Manchester United isn't going to be very active with Zoran Tosic likely to be the only arrival — if contract negotiations can be resolved.
With its title hopes fading, Arsenal's priority is finding a midfielder to ensure it clinches one of the four Champions League spots since inspirational midfielder Cesc Fabregas is likely to miss the rest of the season through injury.
The decline of the pound against the euro could open the way for Premier League players to move to the continent.
But most reinforcements for Italy's top teams are expected not to come from the transfer market but from the return of injured players from Juventus goalkeeper Gianluigi Buffon to AC Milan forward Marco Borriello.
Although Inter Milan is interested in signing Genoa's Diego Milito, one of Serie A's top scorers with 12 goals, or, as an alternative, Sevilla strikers Frederic Kanoute or Luis Fabiano.
Juventus is seeking a defender to replace injured players and is focusing on Fiorentina's Manuel Da Costa, while Liverpool defender Daniel Agger is said to be on Milan's radar.
In Germany, Bayern Munich has said it has no plans for any further transfer activity after signing Landon Donovan on a loan deal through March 15.
Striker Lucas Podolski, frustrated at spending too much time on the bench, had expressed interest in seeking another club, but Bayern has said it does not plan to release him this winter.
Hertha Berlin's key striker Marko Pantelic, whose contract runs out in July, will reportedly be looking for a new club in the transfer window. According to media reports, Pantelic is seeking a euro4 million contract.
It wouldn't be January without Henrik Larsson being linked with a loan move — and Marseille manager Erik Gerets is the latest wanting the 37-year-old Swedish striker.
Marseille needs to boost its attack after dropping to 5th in the standings — six points behind leader Lyon, which is not expected to bring any new players in despite injury problems.
Copyright 2009 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.
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by Vicki
(Meansville, Ga)
August 18, 2013 God seen it necessary to take my Mark on to a better place. We were married for 33 years and the last 5 1/2 years we worked together at our business so were together 24/7 and I would not change that for anything. Mark was a very hard worker, but when he wasn't working he was doing his other passion which was drag racing. On August 15th we left our home in Georgia to go to a race in North Carolina. We got there and Mark raced on the 16th. When he lost the round and got back to the trailer I was standing there as always to greet him and tell what a good job he did, win or lose. This time though he said I need you to help me out of the car, so I did. I said what is wrong, he said I don't feel good. He staggered to the motorcoach. I took care of making sure the car was taken care of and went to check on my Mark. I asked him again what was wrong and he said I think something is wrong with my heart, I said let me go and get the EMTs and he said no, let's load up and head home, I know I am going to end up in the hospital and want to be home.
So we head home with mark driving and after 2 hours he said let's stay at this rest stop for the night. So we did. He was up sick all night and again he did not want me to call for help as we were in South Carolina then. So morning came and he said you're going to have to drive us home. So God had the steering wheel as I sat in the seat because you see I had never in my life driven a 9speed International 8300.
We made it home and I took him straight to the hospital where they immediately did an EKG. We knew the person doing the EKG and he turned to me and said it don't look good. So immediately they got him ready and off we go in an ambulance to a larger hospital who has a special unit for heart patients. We get there and they do X-rays and see that the "widow maker" on the backside of the heart had collapsed. So the surgeon said that part of the heart was dead but that he would be fine because people live like this all of the time. So they put him in a room and as time goes on my Mark asked me " so if I'm going to be okay, why am I still hurting so bad". So I get with the nurse and they contact the Dr. And he orders an sonogram of the heart.
In the meantime my daughter has arrived at the hospital and i have an uncontrollable urge to leave the hospital for a few minutes. So I patted Marks leg and told him I loved him and would see him in a little while, not knowing that would be the last words I would ever say to him. As soon as I left they came in to do the sonogram and he died while this was being done.
My daughter calls and tells me to come back. When I arrive he was gone and the Doctor says he don't know what happened.so we have an autopsy done so we would know. Apparently when he had the heart attack, the heart ruptured and filled the sack around the heart and compressed the heart.
GUILT! That's what I fought for not trying to get help even though he said no. But from going to all of the grief counseling and reading the Bible I have realized that no matter what I had done he would no longer be with me on this earth in body because our days are numbered and his days were up.
As I approach 1 year in 3 days my heart still aches and I miss he terribly. I don't cry every day anymore but I still have many days that he is all I think about many tears flow.
I had a dream about 2weeks ago and his body was on a table and he was standing over it with a huge smile on his face. I have a wedding picture on the mantle and that is the same smile that was in my dream. I know he is with me in some capacity every day. That's what keeps me going as well as my daughter and 2 grand children which my husband loved dearly.
May God touch us all and give us the strength to travel this journey! Amen!
Comments for Mark, Love of my Life, Soul Mate
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SCID is pleased to announce that two Stanford economics PhD students have been selected to receive Ronald McKinnon Memorial Fellowships.
The fellowship honors Professor Ronald I. McKinnon's memory by funding outstanding undergraduate and graduate research in the areas of international economics, international finance, and economic development.
The selection committee received a number of impressive proposals and is happy to announce the 2017 fellowship recipients:
More information about the fellowship can be found here.


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Match Details
- Newlands, Cape Town
ODI debut
TV Umpires
Reserve Umpire
Match Referee
Scorecard Summary
- F du Plessis60 (78)
- JP Duminy52 (59)
- Harbhajan Singh2/23 (9.0)
- Z Khan3/43 (9.2)
Whew, that was a thriller. Some superb bowling from Steyn and Morkel, but that was overshadowed by the lethal hitting of Yusuf, who surely has confirmed a place in the World Cup starting XI, and the nerves of steel of Harbhajan. Two clean hits for six from Harbhajan steered India to victory, just as he had in the high-profile India v Pakistan match in the Asia Cup last year.
South Africa were let down by their batting and the back-up bowling - Parnell is still short of confidence. The positives for SA was the confident debut of Faf du Plessis, though we didn't get to see much of his leg spin.
Post-match presentation: Smith: "Obviously very disappointing, we were some 20 runs short. 240 was a good score on this wicket, full credit to Yusuf whose innings made the difference, the pitch was a bit up-and-down and there was sideways movement as well. Our last five batters managed only 19 runs and lost five wickets in the Powerplay, we could have scored 30-40 runs in that period. We just have to re-group and look to be better than we were today."
Yusuf Pathan is the Man of the Match for his quickfire half-century. "Good win for us. The hundred against NZ has made a big change in my career and also my confidence. If I get the ball in my area, I'd go for it."
MS Dhoni has a big grin on his face. "Very good effort from the lower middle order. The death bowling and Powerplay was also very good from us. We just told the youngsters to play their natural game, we want Yusuf to play the sort of game he plays. Harbhajan is kind of an allrounder for us, though we don't put the pressure on him by calling him an allrounder, and he's been bowling very well, taking the momentum away from the South Africans. Catching has been decently good, we have been outstanding so far, ground fielding has also improved a bit."
Vijay007 says: "With matches like these, who needs to go to the gym to give the heart a good workout?"
Sriram Veera has the bulletin of the match here. Do send us your six-word reports for this game. We'll publish the best ones on Page 2.
That's it from me Siddarth Ravindran, thanks for all the mails. If you are craving more cricket, hop on over to our commentary of the final day of the Pakistan-New Zealand Test which starts in around half an hour.
Slip and short leg in place for Nehra
Botha to bowl, no more options for Smith
END OF OVER:48 | 2 Runs | INDIA: 218/8
- A Nehra2 (9b)
- Harbhajan Singh22 (24b)
- WD Parnell8-0-53-0
- M Morkel10-0-28-3
Two slips in place


text:
Brazil - Apply for Civil Identity Registry (RIC / Registro De Identidade Civil)
From Wikiprocedure
Civil Identity Registry (RIC) is a document similar to a credit card, it has features protecting it from being counterfeited, such as a watermark and the way data is written on the card. The card's chip contains various information, such as height and fingerprints of the holder.
Replacement of the current RG new document began to be done gradually in 2011 and will extend over nine years. Individuals will be selected by the Ministry of Justice and will receive a letter calling for RG replace the old with the new registration card civil identity.
Following are procedures on how to apply at civil identity registry.
Provide eligibility criteria's for going through this procedure.
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Please provide instructions on obtaining the certificate/documents. e.g. The state office holds birth records since january 1908.
Required Information
A list which displays the kind of information which is required to complete the procedure. e.g. 1. Date of Birth. 2. City or County of Birth.
- Identity cards are issued by the state government.
- Submit all required documents.
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Required Documents
- For single persons - birth certificate (the original one or a certified copy),
- For married individuals - marriage certificate.
Explain the fees structure which is required for obtaining the certificate/document.
Explain the time until which the certificate/document is valid. e.g. Birth Certificate Valid Forever
Documents to Use
Please attach documents which can be used by people who would like to follow this procedure.
Sample Documents
Processing Time
Please explain the processing your application.
Office Locations & Contacts
Ministry of Justice
Brasilia, Brazil Map
Phone: +55 61 2025-7987
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Information which might help
Enter other informations which might help
Other uses of the Document/Certificate
Please explain what are other uses of obtaining this document/certificate. e.g. Birth Certificate can be used as proof of identity.
External Links
- Civil Identity Registry (RIC):
- http://www.brasil.gov.br/cidadania-e-justica/2010/12/conheca-o-novo-registro-de-identidade-civil-ric
More information which might help people.


text:
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text:
For parts of this season, the red zone has been more like the no-fly zone for the Ravens' offense.
Baltimore is tied for 23rd in the NFL in red zone efficiency, turning only 12 of their 28 trips inside their opponents' 20-yard line (42.9 percent) into touchdowns.
The Ravens' red zone issues reached a new low in Sunday's win over the Dolphins, when they got inside the Miami 20 on seven occasions, and came away with one just touchdown.
So, why is an offense that has no trouble getting into the red zone - they've made 28 trips into that territory this season - having trouble coming away with seven points on those trips?
"I think it's just execution," head coach John Harbaugh said. "We can do a better job. You know, don't have penalties down there. We want to complete passes. We had a couple opportunities to complete some balls that would have been scores. We could have run the ball better. Miami did a nice job."
The Dolphins have the league's second-best red zone defense, allowing touchdowns on just eight of 25 possessions inside their own 20, so the Ravens weren't facing a bunch of cupcakes on Sunday.
But they did make mistakes which pushed them back and forced kicker Billy Cundiff to trot onto the field and try a field goal. Penalties, sacks, and an inability to move the chains on third-and-short situations led to just one truly successful red zone trip on the afternoon.
"They get paid to stop us and we get paid to score," said wide receiver Derrick Mason, who had the Ravens' lone red zone TD on Sunday. "So sometimes, they win, sometimes we win. But, I think if we do not do the small things, the little mistakes, then we will score more often than not.
"I think often times, we've gotten down there and we've done something to kind of push ourselves back out of the red zone, so in the second half of the season, we have to start to minimize the penalties and minimize some of the mistakes we have down in the red zone."
Wide receiver Anquan Boldin said that while the Ravens only came away with one touchdown on Sunday, there were times when the situation dictated that all they really needed was a field goal.
Instead of forcing something and risking turning the ball over, Boldin says that field goals can sometimes get the job done.
"There's certain things that come to play in the red zone," wide receiver Anquan Boldin said. "We felt like at certain points of the game we had the game under control. There's certain times where you just want to preserve a field goal and that's what we did. You have to be smart."
But for every time that the Ravens played it safe and opted to simply kick a field goal to go up by 16 points (like they did late in the fourth quarter), they also had a possession where they failed to turn a great opportunity deep in Miami territory into seven points.
The possession where Lardarius Webb returned an interception to the Dolphins' four-yard line comes to mind. After Webb's return set the offense up in great shape, Joe Flacco got sacked twice and a delay of game penalty forced a Cundiff field goal try. The Ravens' kicker never even got his foot on the ball because of a Sam Koch botched snap, and Baltimore came away with nothing.
Running back Ray Rice referred to playing in the red zone as "playing arena football" because there are so many players running around in such a condensed area, and it's definitely not an easy task to come away with touchdowns most of the time.
But the Ravens know they need to be better in that area if they're going to be successful in the long run this season.
"We understand that we're going to get down there a lot as a team," Mason said. "What we have to start doing now is capitalizing, and in order to capitalize, we have to make sure that we don't have to do anything to shoot ourselves in the foot."


text:
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Via: Refinery 29 By: Erin Cunningham Photos by Kristiina Wilson Tis the season to be in love. And, with New York City being the epicenter of all things romance, especially during the holidays, who better to turn to for style insights than eight of our favorite couples? We asked the most adorable pairs across(…)
Interviews / Guests
Urban Bush Babe: Hannah Faith
Tell us your name and where you are from? I go by the name of Hannah Faith I'm from England, North London to be a bit more specific. Photo: Rianna Tamara What do you do? Is there a resistance or compatibility with your hair in your field of work? I'm a DJ, been(…)
via: ASOS by: Nellie Eden Photos: Joey Rosado Think you know what NYC cool looks like? Think again. Meet the Quann sisters: TK Wonder, a singer, and Cipriana, a blogger and co-founder of website Urban Bush Babes. Take a peek into the stylish lives of the Brooklyn-based, Baltimore-raised identical twin babe-sisters. Why? They have the kind of(…)
Featured Photo: Brad Ogbonna Tell us your name, occupation and where you are from? Q: My name is Quinn Wilson, I am from a tiny town in Michigan called Haslett. A: Asha Efia, I am a photographer and I was raised in Las Vegas, NV. I lived in Minneapolis for a(…)
Music: Marsha Ambrosius Talks Hair w/ Cipriana Quann
via: Greenroom Magazine Interview: Cipriana Quann "With or without it you have to be passionate about what you do. You have to care about your dreams enough to pursue it. If there was one person that was like, nah, and that stops you, then you don't really want it." -Marsha Ambrosius (…)
Urban Bush Babe Fatima Osman
Tell us your name and where you are from? My name is Fatima Osman. I'm from a lot of places, really. My parents are born and raised in Sudan, like their parents. I myself was born in Saudi Arabia. When I was five years old we moved to Sweden as my dad got the opportunity(…)
Artist: Ojo Agi
Tell us where you are from, the early years of your childhood and what influenced your direction towards the arts? I was born in Canada to Nigerian parents. I think a lot of my childhood experiences would be shared by other individuals who grew up under immigrant parents. There's a lot of pressure to(…)
W Magazine: The Quann Sisters Love Vintage, Lisa Bonet, Being Comfortable, & Other Stories
The fashionable duo, who recently collaborated with & Other Stories, share a page from their style notes. via: W Magazine by Sarah Leon Photos: Charlotte Wales TK (aka TK Wonder) and Cipriana Quann, who is often referred to as Urban Bush Babes, are well known for their distinct personal style and modeling gigs. (Their most recent,(…)
Watch Cipriana Quann in Refinery 29 x Secret's #Epic 48 Weekend Short Film
via: Refinery 29 by: Alison Ives Sponsored by: Secret This Internet-Preneur Gives Us NYC Like We've Never Seen It Before Some look to the weekend for a little R&R, but to New Yorkers, that concept simply doesn't exist. With a steady stream of concerts, gallery exhibits, and new restos and bars to check(…)
Tara Purnell: WTRMLN WTR (Watermelon Water)
Photos: Cipriana Quann *Click photos to enlarge Tell us your name, occupation and where you are from? My names is Tara Purnell, hailing from our nation's capital Washington, DC. I spread love and make magic with WTRMLN WTR, yoga, and music. What was the inspiration or idea that sparked Watermelon Water? My(…)


text:
- Compatible XF Versions:
- 1.4
- 1.5
- Visible Branding:
- Yes
(Download and unzip)
1. Go to Admin
2. Select Languages
3. Import languages
4. Import As... select Child of language: and No parent
(Download nad unzip)
1. Go to Admin
2. Select Languages
3. Import languages
4. Import As... select Overwrite language: and select Hrvatski
Note: Language file have name language-Hrvatski.xml. Do NOT upload language-Hrvatski.zip. First unzip.
Hrvatski prijevod za XenForo 1.5.x.Frontend je uglavnom preveden, administracija je manji dio prevedena.
Croatian Translation for XenForo 1.5.x. Fronted is translated to a greater extent. It is possible that some details were left untranslated.
Supported plugins (not full translated):
1. Control "nofollow" URLs
2. Similar Threads
3. Tapatalk
4. Thread Ratings
5. Special Avatar for Banned Members
6. TPU: Detect and Block Spam Registrations
Demo: Hedonist385.com forum
Unmaintained Croatian Language Pack 1.5.0
Croatian translation for XenForo
Recent Updates
- 1.5.0 Aug 18, 2015
- Support plugins Jun 29, 2015
- Update to 1.4.7 May 19, 2015


text:
It only takes a minute to get started. Fill out our simple sign up form and login to the website.
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dump file option
I am simulating a transient flow using cfx4.3. I want to store in the dumpfile only temperature. I define that in the dumpfile options >> Temperature >> Each time step. I want only temperature inorder to use short processing time in matlab for postprocessing. But later on I found that other variables are also stored in the dump file, like u,v,w velocities, epsilon, k.... does anyone knows how to store only Temperature? Thanks Tad
|All times are GMT -4. The time now is 11:56.


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SKU: bkn1h
Availability: In Stock
Origin: Burkina Faso
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Is putting on a little dog-and-pony show every now and then detracting from the feminist cause?
What is clear is that this trend is detracting from our system of government.
He storms and rages at his detracting pupils; but ends with roars of laughter at their impudence.
Send to table without adding or detracting from their flavour.
The river, in full freshet, was very muddy-looking, detracting much from the beauty of the rapids.
There is as a vice in praising, and as frequent, as in detracting.
This is not detracting from the French success, which was complete and masterful.
There was one detracting element which certainly impaired its charm, the "Quid amarum," amidst all its excellence.
It adds to the color of Mr. Kingsley's pages, while detracting from his authority, that he is always and inevitably a partisan.
Any attempt to free thee from the scorn of the rabble would result in detracting from thy singular and peculiar praise.
early 15c., from Middle French détracter, from Latin detractus, past participle of detrahere "to take down, pull down, disparage" (see detraction). Related: Detracted; detracting.


text:
The world was stunned last week when Hollywood A-lister Charlie Sheen admitted that he is HIV positive. Now, his ex-girlfriend Bree Olson is demanding Sheen face criminal charges for putting her at risk by not disclosing to her that he has HIV.
According to Daily Mail, the former porn star Olson said "face the consequences" for exposing her and countless others to HIV. Sheen told the world he was diagnosed with the virus four years ago, which left Olson terrified considering the fact she was living with him in 2011. Despite this, he never told her about his diagnosis.
Now, Olson is accusing her former boyfriend of "putting a loaded gun to my head" by exposing her to the disease. Since exposing someone to HIV without telling them is a crime in the US, she thinks he should be in jail.
"Now he has to pay for what he did. It's unforgivable," she told The Sun On Sunday.
Hours after Sheen revealed his diagnosis on the Today Show, Olson appeared on The Howard Stern Show to tell her side of the story. She told Stern that she remembers that Sheen was plagued with night sweats when they lived together, which is an indicator of HIV.
"That was when I was with him. I was living with him. We were sleeping together every single night," said Olson. "And he never said a word."
Olson went on to say that Sheen would demand sex twice a day, and that he used lambskin condoms, which offer less protection than regular condoms.
"I didn't want to use lambskin, I wanted to use regular condoms. I made the same mistake that a lot of people do, I trusted him," Olson recounted. "There were times when we didn't use condoms, because it was only to prevent against pregnancy. I loved him, yes, he was my boyfriend. I left the [porn] industry for him and I never went back."
Olson added that she has been tested for HIV recently, and that she is negative.
Do you think Sheen should be in jail? Let us know your thoughts in the comments section.


text:
|Reviews for The Last Dance
| Skykiddrio9762 chapter 3 . 2/8/2013
there is the meta crisis who is the other universe with stupid rose, he's half an half.
| Deanna chapter 3 . 12/30/2012
Don't read this. Read this and you're dead. This is emotional. More emotional than you could ever believe. Don't feel terrible sadness. Don't get emotionally traumatized. And don't read this story. Good luck.
Seriously, great story. Thanks for messing with my emotions :)
| InvisibleBlade chapter 3 . 11/8/2012
So beautiful x
| Martin-J-Christopher-Freeman chapter 2 . 11/8/2012
Love this x
Please update soon
| Maribor chapter 3 . 9/20/2012
Absolutely lovely. Very well done. :)
| Sylva Dax chapter 3 . 5/15/2012
It was Rory who made my eyes sting. Knowing what he was going to find I had to force myself to keep reading.
| notwritten chapter 3 . 5/12/2012
Beautiful but sad. Keep smiling. :-)
| monkeymail chapter 3 . 5/12/2012
is he going to save her eventually or are you going to leave it like that? i like this way but i think it could go either way cause wasn't there all of that information on River's dna on deamon's run so you could do something like that...but you don't have to i just like to think that the Doctor should get a happy ending with River...loved it, it was written beautifully please update again soon
| Citizenofwhoville123 chapter 3 . 5/12/2012
SO SAD! But beautiful and sweet too, especially the bit with Rory. I loved it! Good job!
| Keeter chapter 2 . 5/6/2012
Gah! Don't stop! MORE!
| Sylva Dax chapter 2 . 5/6/2012
I was definitely not expecting the Doctor to contact River as a node. Getting out the tissues for the next chapter.
| Citizenofwhoville123 chapter 2 . 5/6/2012
Are you going to cry...happy tears? Somehow I doubt it, but at least he sees her again. This story is beautiful so far! Please post the next bit soon!
| SheWillBeAmazing chapter 2 . 5/6/2012
Love it. Can't wait for the next part.
| DR4G0lV chapter 1 . 4/4/2012
Well done. Looking forward to the next part (or two)!
| monkeymail chapter 1 . 4/3/2012
awww is he going to see river again? loved it please update soon