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- Home
- Graduate Program >
- Computer Graphics Design
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Computer Graphics Design Graduate Program
A Computer Graphics Design Graduate Program from RIT will put your career on track, jump-starting your entry into the world's high-paying, innovative technology industries. At RIT, you'll discover more than 80 Graduate Programs in some of the most respected math and science-related disciplines. So don't wonder anymore: contact RIT today to learn more about the career-focused programs we offer!
The world is waiting - so study at RIT
Enroll in an RIT program and you'll find that a world of opportunity opens up. Our partnerships with countless national and international corporate and government entities will give you unparalleled opportunities for research and career placement, while our faculty members are internationally respected for important contributions in their respective fields.
Completing a Graduate Program in Computer Graphics Design at RIT can also help to enhance your earning potential. In 2014, RIT had a 95% career placement rate for graduate students! Above all, when you are armed with an education from RIT, you'll have the chance to network with with more than 100,000 well-connected alumni respect the power of an RIT qualification and often look to hire graduates of our programs.
At RIT, every student has access to top-notch academics, great facilities and a world-class faculty. Our graduates confidently transition into the "real" world feeling that what they've learned at RIT will help them in life, and most importantly, give them an advantage in the job market. We can provide you with options that will allow you to earn your computer graphics design qualification in the way that works best for you. Ask about our quarter program, which allows you to take classes four times a year and earn your degree faster.


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Title: Photo Against Photo
URL: http://www.photoagainstphoto.com
Description: PhotoAgainstPhoto hosts thousands of photos taken at the same time with different cameras or lenses. Photos can be compared side by side.
Date Submitted: 12/10/14
Listed / Last Time Edited: 12/10/14
Main Category: > Cameras and Equipment
Second Category (if any):
Linkedin (if any): http://www.photoagainstphoto.com/links/
Number of visitors we sent to this photo site: 167 time(s).

subset: commoncrawl


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Oh she such a doll, Heather!!
Thank you Ladies! I will have to take some recent pictures she sure has gotten a lot bigger .
So precious!
Thanks so much !!
About Heather Smith
Current: Brooklyn, New York
Birth: July 06
On Moms.com since: Jul 16, 2014
a 21 year old mother of a baby girl. Wise beyond my years but still open to learning new things. I love reading and sopping. Whether groceries or clothes I enjoy and love being a Mom. Can't wait to get married and have more babies Haha in the future.


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Rank Your Top 10 Barcode Characters
I enjoyed writing my series for one reason: The characters. At times, I felt like crying with them or their anger boiled in my chest. I supported them fully in their endeavors and hated each villain that opposed them. At the same time, I enjoyed constructing villains with more noble intent than my protagonists. The series was fun to write overall. However, I want to rank the top ten and compare it to your list. Here are my favorite characters in order of least to greatest: Arnold Mishimoto Starting of…Read More


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Radically Saved (12 of 33) by Jerry Vines
This content is part of a series.RADICALLY SAVED (12 of 33)
Jerry Vines
Acts 9:1-30
I find it hard to keep up with words and phrases and
their meanings today. I hear somebody say, "Man,
you're bad." Where I came from when you said somebody
was bad they probably belonged in a jail. But today
when somebody says you are bad that means you're
pretty cool dude. Then I hear people say, "You're
'the bomb.'" When I used to hear people talking about
a bomb I started looking for a shelter. Today when
they say you're the bomb that means you are really
something else. A term I have heard in recent days to
describe what it means to have a personal salvation
experience is say he is "radically saved." When I
came along we used different terminology. We talked
about a conversion experience. We talked about an
experience of grace. But I hear more and more people
today saying about someone, "He is radically saved."
I looked up the word "radical." Before I accept this
I have to find out what that word "radical" means.
One of the definitions I found was "an extreme change
of views and habits." So to be "radically saved"
means that a person has had an extreme change of views
and habits. There have been, according to that
definition, some people who have been ""radically
saved" throughout the history of the Christian church.
I have told you about the salvation experience of St.
Augustine, after whom the city is named. I have told
you about the experience of John Wesley. Both of
those in that terminology could be described as
"radically saved." The conversion experience of
Charles Colson we would say that this man was
"radically saved." We have people in our congregation
who have had such an experience. I saw George Contois
this morning coming in. George was a person who was
"radically saved." By his own definitions he was not
an alcoholic, he was just an old drunk. He met the
Lord Jesus Christ watch ...
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- Product Description
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Product Description Specification Reviews Downloads FAQ's Questions (3)
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Did GIs "Fake Out" German and Japanese Soldiers with False M1 *PINGS*? Bloke Explains Why Not
If a Garand pings in the woods, and no one is around to hear it, did it make a sound? The answer is "yes", because German super-hearing allows them to detect high-pitched noises from up to a kilometer away! The funny thing about myths is that sometimes [Read More…]


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Quiz: Can You Give Your Old PC a Boost?
You have a dozen applications running, but you only recognize three of them. You should:
Your browser has become unusably slow. What's the likely cause?
Viruses slow down your computer by:
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Looking for more speed? Visit EasyTech to find out how to bring out the best in your PC.
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Grovetoberfest: It's Raining Beer (Pictures)
Thousands enjoyed Grovetoberfest - even in the rain
All photos by Laine Doss
Dark skies and rain this past Saturday didn't deter thousands of beer enthusiasts from enjoying Grovetoberfest in Coconut Grove's Peacock Park.
As the weather shifted from cloudy to drizzly to full-on pour, beer drinkers gathered under the giant tents to sample hundreds of beers and ales, watch cooking demos and eat at several food trucks.
The only casualty of the day seemed to be the bands on the main stage, who played to only a brave few who stood out in the rain to watch (though many could be seen dancing to the music from the shelter of the beer tents).
Though lines were long, they moved quickly, allowing everyone to get a taste of their favorite beers and try new ones like Schnebly's new Gator Tail Ale (with sour passion fruit notes) and mango hard cider.
We're looking forward to next year's Grovetoberfest...hopefully with Mother Nature's full cooperation.
gastroPod's Jeremiah and Carla served up a special beer pairing menu at Grovetoberfest.
Though dark skies threatened rain, the crowd was not deterred from their goal of drinking beer.
Scott Rodgers from Miami came dressed for the occasion.
Hatuey sampled its beer, which was recently reintroduced to the American market.
Chef Pastor Sequera conducts a cooking demo at Grovetoberfest.
The tents got crowded during a brief downpour, as beer enthusiasts sought shelter from the rain.
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Digital guardian will protect you online
Identity theft protection by cognitive systems
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"Bank accounts hacked!"
"Credit card numbers compromised!"
"Social media network passwords stolen!"
These headlines are the norm on the news ticker these days. And if your identity has ever been stolen, you know exactly how difficult it is to recoup and rebuild your digital self. IBM Research is building a digital guardian to soon learn how to better-secure your online life.
Peer-reviewed Journal Articles
- Journal of Food Protection: Framework for Managing Mycotoxin Risks in the Food Industry
- Science Direct: A modeling framework to accelerate food-borne outbreak investigations
- Science Direct: Geospatial Resolution of Human and Bacterial Diversity with City-Scale Metagenomics
Over the next five years, this guardian of big data will analyze and learn from your online behavior patterns, going back months and years to know what to protect. And when it detects a possible breach, you will be the first to know.
This cognitive system currently tracks several different security anomalies. It also incorporates security measure such as fingerprint and facial recognition. So, as it understands what you secure and how, it can even make decisions for you, per your instructions and permission. Not trying to buy a jetpack because you're afraid of heights? Your digital guardian will know this, and won't let this daredevil with your credit card buy it either.
Protecting your patterns
Hopefully, it won't come to the point of a breach in the first place. IBM and its partners are layering in "always aware" intelligence. You can't be in two places at once. So, if the smartphone you accidentally left at a restaurant is being fondled by fraudulent fingers, the pervasive system will recognize the offender's different touch pattern (even if your phone is unlocked) and lock your account.
In another example, imagine two purchases: $40 at a gas station, and $4,000 at Tiffany & Co. Today's fraud monitoring might see the diamond purchase as highly suspicious, and ignore the charge at the pump. But your digital guardian will know that your car has a near-full tank of fuel; that you don't usually re-fuel until you're down to about one quarter tank; not to mention that you're at the office when this charge appears. It will also know that you've been shopping for an engagement ring and have been spending your lunch hour window shopping outside the store.
This and other emerging learning systems will know you, help you, and protect you as we continue to generate more and more data, and put more and more of our lives online.
Learn more about IBM security solutions
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IBM & Cognitive In The News
- Forbes: Why Open Beats Closed, September 2016
- TechCrunch: IBM and MIT partner up to create AI that understands sight and sound the way we do, September 2016
- Boston Globe: MIT and IBM form to teach computers recognize sound, images, September 2016
- The Stack: IBM and MIT collaborate to advance AI machine vision, September 2016
- TechCrunch: The White House requested input on artificial intelligence, and IBM's response is a great AI 101, August 2016
InformationWeek: IBM: AI Should Stand For 'Augmented Intelligence' August 2016
- Economist Special Section on AI: From not working to neural networking; Frankenstein's paper clips, June 2016
- Washington Post: Everything you think you know about AI is wrong, June 2016
- Bloomberg TV: Forward Thinking: March of the Machines, June 2016
- TIME & Fortune: IBM Researcher: Fears Over Artificial Intelligence are 'Overblown', May 2016
- IBM Announcement: IBM and the University of Illinois to Pioneer Next-Generation Cognitive Computing Systems, April 2016
- CNNi: IBM Watson and the future of artificial intelligence, March 2016
- IBM Think blog: Extending Game-Based AI Research into the Wild, March 2016
- TIME: IBM's Top Researcher: A Win for Computer is a Win for Humans, March 2016
- CBC (Canada): Watson and the Rise of Cognitive Computing, January 2016
- Financial Times video: Robots in the workplace, January 2016
- IBM Announcement: IBM Research and Rensselaer Polytechnic Institute Pioneer Next-Generation Cognitive Environments for Business Decision Making
Meet the researcher
J.R. Rao
Director, Security Research
T.J. Watson Research Center
Peer-reviewed Journal Articles
- Journal of Food Protection: Framework for Managing Mycotoxin Risks in the Food Industry
- Science Direct: A modeling framework to accelerate food-borne outbreak investigations
- Science Direct: Geospatial Resolution of Human and Bacterial Diversity with City-Scale Metagenomics
Read more about our other 2013 "IBM 5 in 5" predictions:
The classroom will learn you
The classroom of the future will learn about each student over the course of their education, helping students master the skills critical to meeting their goals. A system fueled by sophisticated analytics over the cloud will help teachers predict students who are most at risk, their roadblocks, and then suggest measures to help students overcome their challenges.
Buying local will beat online
Buying local will be back in style once again. Savvy retailers will use the immediacy of the physical store and proximity to customers to create experiences that cannot be replicated by online-only retail. They will magnify the digital experience by bringing the web right to where the shopper can physically touch it.
Doctors will routinely use your DNA to keep you well
Computers will help doctors understand how a tumor affects a patient down to their DNA, and present a collective set of medications shown to best attack the cancer, while reducing the time it takes to find the right treatment for a patient from weeks and months to days and minutes.
The city will help you live in it
Smarter cities will become sentient cities, understanding in real time how billions of events occur as computers learn to understand what people need, what they like, what they do, and how they move from place to place. Mobile devices and social engagement will enable citizens to strike up a relationship with their city leaders so their voices will be heard not only on election day, but every day.


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Beware Of The Warning Signs Your Headaches Have Been Giving You
May 22, 2016
Everyone has those days when they feel like their head is going to explode into millions of bits. It aches so terribly that you have no idea whether to bang it somewhere or simply take a pill. But have you ever given it a thought that your headache might be telling you something. That there are certain signs you shouldn't miss on.
Well, people you are surely in for a ride. Here are the indications your headache has been giving you and you shouldn't miss on them.
1. Tension headaches.
These are the kinds that won't leave you worrying. All you have got to do is pop a pill and you are good to go.
2. Migraines.
These range from mild to terribly painful. Plus, it lasts for a longer period of time. The reason behind it is yet unknown but in short, consult a doctor.
3. Cluster headaches.
One of the most severe kind of headaches, these are terrible. The person suffering from it has piercing pain in his/her eye, feeling like it might burn. One cannot sit still while experiencing it.
4. Sinus headache.
It is a result of sinus inflammation, generally affecting the area surrounding the bridge of the nose and cheekbones.


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Job Description: The American Red Cross is seeking Medical Directors for our Blood Services Division in these locations:
Our blood donors are ordinary people – high school students, factory and office workers, business executives, parents and grandparents, and people from every walk of life. But they share common motivations – a generous spirit, a desire to give back to their community and to help others. If these are values that are important in your job search, we encourage you to apply for a Medical Director position at the American Red Cross today to learn more.
What do the best Red Cross physicians do differently than your typical Transfusion Medicine physician?
What does a Red Cross Medical Director need to do to be successful?
What needs to be done first?
There are a lot of blood banks – what makes the Red Cross different?
**If the final candidate selected is out of area, relocation will be offered.
Responsibilities: Medical Consultant in Transfusion Service, Patient Services, Patient Blood Management 1. Provide medical consultation in transfusion medicine, blood banking and patient services to hospital customers. 2. Organize and participate in regional Medical and Technical Advisory Committees or equivalent programs for hospital customers. 3. Provide continuing medical education to external customers and internal audiences. 4. Promote the safe and effective use of blood components through interactions with hospital customers, and participation on transfusion committees and professional organization committees (i.e., AABB, CAP). 5. Direct patient service programs including but not limited to therapeutic apheresis, stem cell collection and processing, perioperative autologous cell salvage, and patient blood management.
Medical Oversight of Blood Center Operations and Regulated Activities 6. Direct the medical aspects of regulated blood center operations including collections, donor management, recipient adverse reactions, suspect product investigations, and other operational systems. 7. Direct investigations of donor complications and recipient transfusion reactions by interacting with the Donor and Client Support Center to ensure timely completion, documentation and reporting to BHQ hemovigilance program. 8. Participate in the development of national procedures, policies or position statements on medical criteria for donor eligibility and safety, component quality, and transfusion practice. 9. Serve as CLIA Laboratory Director for regulated collection activities in assigned regions, immunohematology laboratories, HLA laboratories, stem cell collection and processing, and/or national testing laboratories. 10. Investigate concerns and complaints related to donor complications, customer service, or hospital relationships including notification of Executive Leadership, as necessary; plan and implement appropriate corrective actions.
Perform other duties as assigned.
The above statements are intended to describe the general nature and level of work being performed. They are not intended to be an exhaustive list of all responsibilities and duties required of staff so classified.
Scope: Divisional Medical Directors perform all the medical roles and functions related to regulated blood center operations, patient services, and laboratory supervision in the regions to which they are assigned. • Interact with hospital customers, donors and staff to provide consistent, timely and comprehensive medical guidance and information. • Perform medical and clinical duties to support regional or divisional activities and ensure adequate coverage in all domains. • Provide and develop Patient Services programs in the region. • Provide medical expertise and expert opinion to other internal and external audiences, including but not limited to operations, sales and marketing, the Office of General Counsel, regulatory agencies, and public inquiry. • Participate in BHQ continuing medical education programs.
The position also includes medical supervision and collaboration with the research laboratory co-located with the regional headquarters. Duties will include providing support for all protocols involving blood donors, radiolabelling of components, and transfused blood product recovery studies. Prior experience or training as a radiation safety officer or authorized user is ideal, but training will be offered.
The Divisional Medical Directors will be expected to travel between the regions to which they are assigned, as little as 20% but up to 50% of their time as needed.
Qualifications: • M.D. or D.O. degree with post-graduate training/specialization in blood banking/transfusion medicine is required. • Board certified or eligible in internal medicine, pediatrics or clinical pathology. • Board certified or eligible in blood banking/transfusion medicine is highly desirable. • Extensive knowledge of current/projected trends in transfusion medicine/blood banking and hospital health care highly desirable. • Knowledge of medical/technical principles of blood center operations. • Must be licensed in the state of the primary site of the region and all states served by the region and/or Division, as needed. • Minimum five years experience in blood banking/transfusion medicine, or related field. • Completion of a blood banking/transfusion medicine fellowship and board certification in blood banking/transfusion medicine, hematology and/or internal medicine are desirable. • Experience in relevant medical/scientific research and scholarly activities sufficient to warrant academic appointment is highly desirable.
To learn more, send your resume or CV to firstname.lastname@example.com. For proper routing, please indicate location of interest in your subject line.
The American Red Cross is an Equal Opportunity/Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to sex, gender identity, sexual orientation, race, color, religion, national origin, disability, protected veteran status, age, or any other characteristic protected by law.
An engaging three-day event you won't want to miss.


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Someone asked me that question some weeks ago.
Back in 2005 you were able to enable/disable CLR integration from SAC's GUI (SAC being Surface Area Configuration). Of course you could do it programmatically via sp_configure or via Microsoft.SqlServer.Management.Smo.Configuration.IsSqlClrEnabled property which is what SAC leveraged underneath.
Starting with 2008 SAC was removed and the justification for that decision is that what you could do from it was only a subset of what you can do now with PBM (PBM being Policy Based Management). You can now set CLR integration either through the Server Configuration or the Surface Area Configuration facets. Both of them expose a ClrIntegrationEnabled property. They both still leverage the IsSqlClrEnabled property mentioned above, which queries sys.configurations to get the current values and executes sp_configure to set them.


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Manuel Guerra Skin Care & Juice Bar
Unit 3, The Artworks
Elephant Road
07533 212342
Manuel Guerra Skin Care & Juice Bar
Unit 3, The Artworks
Elephant Road
07533 212342
Manuel Guerra Skin Care & Juice Bar
Unit 3, The Artworks
Elephant Road
07533 212342
Welcome. My name is Danielle. I provide high quality and effective soft tissue therapy and injury rehabilitation as well as offering a regular deep tissue, full-body conditioning and a pregnancy and post-natal massage service.
Whether you are troubled by neck tension or suffering lower back pain, recovering from a recent or an old injury, wishing to improve flexibility, feeling the discomforts of pregnancy or simply wanting to unwind and de-stress, each of my sessions is developed specifically in line with your treatment goals.
During sessions, if appropriate, I can explain what I find, helping you to more effectively understand and manage your own condition. I place great importance on evaluation through touch to accurately identify and manage lines of stress.
If you are suffering with musculoskeletal pain, or have a specific remedial issue, I work to identify and correct any underlying postural causes and determine what other factors may be contributing to your complaint.
Alternatively, if you are looking for something more general, I offer regular deep tissue massage: A full body conditioning for those who wish to maintain good overall well-being, keep on top of minor aches and pains and generally unwind and relax. Soft tissue therapy is not only suitable for injury rehab and prevention it is also key to keeping your muscles soft, supple and strong. Tensions within tissues are evident to the touch and for that reason they can be accurately identified and managed. Evaluation through touch helps guide my work. Your entire body is worked on, freeing up areas of restriction and tension, keeping you flexible, and generally improving your overall physical condition. This helps to encourage good posture, allows you to move more easily and keeps in check minor aches and pains before they get the chance to develop into full blown injuries. On a regular basis these sessions are an excellent way to keep you feeling at your best.
Working out of various locations, I cover the Elephant and Castle, Borough, London Bridge, Southwark and Blackfriars areas.
I offer a free telephone consultation to discuss how these sessions can best help you. You can telephone me on 07533 212342, or alternatively, email me at email@example.com.
Discounts apply when regular or block sessions are booked.
Gift vouchers are available.
My Experience
I've worked successfully with a wide variety of people, from elite athletes through to 60-hour-a-week office workers suffering from postural complaints. I hold the BTEC level 5 Diploma in Sport and Remedial Massage. I'm a member of the Sports Massage Association (SMA) and registered with the Complementary and Natural Healthcare Council.
Member organisations
Accredited register membership
Additionally, I offer...
My Rates:
60 minutes ........................... £ 45
90 minutes ........................... £ 65
Discounts apply when regular or block sessions are booked. Gift vouchers are available.
Marta Modesto, Admin assistant
"Danielle was amazing, she found all of my trouble spots, took some pain out of my body and helped me with my posture. I've already recommended her to my co-workers… I loved the experience!"
Anna Garber, IT trainer
"I used to see Danielle when she practiced solely as a deep tissue masseuse but having had a recent hip replacement which left me with one leg slightly longer than the other and causing me a lot of pain, I decided to make another visit to see her again, as she was the only person who had ever made any real difference to my aching back.
At this point I was using a crutch and also waiting for my 2nd hip to be replaced. She explained that she works differently now and I had to do this whole consultation and assessment thingy… All I wanted was a back massage!!! Anyway, I agreed.... She said 'For now, I am NOT going to touch your back'. I thought, doesn't anyone ever listen to me!!! However to my surprise when she went in through my core muscles I felt this wonderful stretch in my back and incredible tension release. Literally, the next day I stopped using my crutch and I have been seeing her ever since!"
Daniel Hooker, Commonwealth Games, ambient 100M sprinter
"In my ultimately successful attempt to gain selection for the England team at the 2014 Commonwealth Games in the T37 100m, few people have been as significant as my masseur Danielle Barnett. I suffer from hemiplegia as a result of cerebral palsy, which leaves me with very tight muscles. Danielle's expertise has been vital in loosening those muscles to provide me with the range of movement necessary for high level sprinting. She has also engaged in longer-term work to lengthen my hip flexors in order to take pressure off my lower back, which had suffered from niggling pains for much of the previous year, as well as working with my physio to ensure swift recovery from a knee injury sustained only ten weeks before the Games themselves. Writing this three weeks before my competition, I feel as loose and fast as I ever had, largely thanks to having doubled the number of massages I have with Danielle!"
"… Thanks Danielle, thank you as well for your role in getting me there, I did a decent job all told."
Yoginee Patel - Career Coach
"I was training for the New York City Marathon when I started getting stabbing pains in my legs. In search of relief, I found Danielle. She was a life saver. By the time I got to her, I was struggling to finish my runs due to the pain. Well, Danielle really got into the legs and I can honestly say that I would not have been able to continue my training or run the marathon was it not for the work that she was doing.
The runs I did after a massage were the best I had. My legs felt lighter and looser which enabled me to go further. I highly recommend Danielle and she will always be on my speed dial whenever I'm training for these events."
If you have been diagnosed with or suspect you may have a pre-existing medical condition you should consult your GP for advice, diagnosis and treatment and always inform your health professional before starting any alternative or additional therapies, treatments or making any major changes in your diet or exercise programme.
|Sign language:
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Hours of business: Monday - Friday, 8am - 9pm. Also operating at weekends. To make a booking, please telephone or email me.


text:
Today's Devotional
The OneRead Galatians 6:1-3 and consider the importance of helping other believers in Jesus.
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Monday, October 18, 2010
GENBAND Wins Saudi Arabian Customer
Plano-based GENBAND reports today that it has signed a Saudi Arabian customer for its equipment. GENBAND said that Digital Star, a new Saudi Arabian service provider, as well as related Egyptian subsidiary 1Phone, have selected the firm's hardware for use in the Middle East. Financial details of the win were not disclosed. Digital Star is offering up hosted applications to enterprises and carriers, and is using GENBAND's products for its IP gateway products.


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2001 gmc sierra
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APHIAplus (AIDS, Population and Health Integrated Assistance), Kamili
Community health worker, Faith, demonstrating different HIV med containers. Faith worked to provide a visual medium for separating the family's medications, as the mother could not read.
U.S. Presidential Initiatives: - Global Health Initiative - President's Emergency Plan for AIDS Relief Funding Level: $100 million Duration: January 2011 December 2015 Activity Goals: - Increase use of and demand for quality health services, products and information - Scale-up and expand community and facility health service delivery especially to marginalized, poor and underserved populations - Strengthen water and sanitation programs - Strengthen food and economic security of households caring for orphans and vulnerable children - Address gender issues in health services - Design and implement effective behavior change communication programs Activity Accomplishments (2013): - Supported 134,619 HIV orphans and vulnerable children - Tested 83,427 women for HIV, identified 2,135 HIV-infected women and provided ARV prophylaxis to virtually all of them and their exposed babies - Reduced mother-to-child transmission to 8 percent
ACTIVITY OVERVIEW USAID Kenya supports an integrated service delivery model to improve the health of Kenyans across the country. The AIDS, Population and Health Integrated Assistance program, also known as APHIAplus, combines family planning, maternal/child health, malaria, nutrition, tuberculosis and HIV/AIDS prevention, care, and treatment services to provide an integrated, high-quality, equitable approach to sustainable services at the national, county, and community levels. Integrating these activities through one program provides more effective communication and coordination with county health administrators. Seamless services and technical support at the local level ensure health workers address the unique needs of each geographic area across the country. ACTIVITY AREAS APHIAplus Kamili, Kamili means complete or perfect in Kiswahili, works with the government and community-based organizations to: Ensure access to quality health services and products APHIAplus Kamili builds the capacity of health care providers to deliver high-quality health services. Specifically, the activity is helping people living with HIV/AIDS gain access to counseling and treatment, and is reducing child deaths through improved maternal and newborn child health services at health facilities. Improve the well-being of the poor and marginalized groups At the community level, the activity is strengthening water, sanitation and hygiene activities and is improving the food security and economic well-being of households caring for orphans and vulnerable children. It assists orphans and vulnerable children with education, life-skills training and suggestions for income generation. ACTIVITY IMPACT When community health worker Cecily Wanyaga promotes healthy behaviors and HIV prevention and treatment services among her neighbors, she speaks from her heart—and experience. Community health workers like Cecily learned about access to HIV prevention and treatment services through the work of APHIAplus Kamili. This training allowed Cecily to educate her
- Currently supporting 24,055 patients on ART treatment
own community about the importance of adhering to HIV drug regimens and maintaining good nutrition.
Activity Locations: 11 counties in central and eastern Kenya namely; Nyeri, Nyandarua, Kirinyaga, Kiambu, Murang'a, Embu, Machakos, Meru, Tharaka-Nithi, Makueni, and Kitui.
Now, Cecily goes from house to house, visiting close to 100 people every month. She provides health education on homebased care for HIV, as well as referrals to health facilities. Cecily also spreads awareness about HIV treatment at public gatherings, churches and youth groups. "People living with HIV can identify with me—that helps to convince them to return to care and treatment," said Cecily, adding that she has so far convinced three HIV-positive women to give birth at the hospital instead of at home. All three women delivered HIV-free children. Cecily credits APHIAplus Kamili with allowing her to feel comfortable disclosing her own HIV status. "The ability to disclose my status has helped our community a lot," said Cecily, "we formed a support group and the numbers keep increasing each time we meet."
Key Partners: Ministry of Health, National AIDS and STI Control Programme, Division of Reproductive Health, Ministry of Labor and Social Services JHPIEGO, National Organization of Peer Educators, African Medical and Research Foundation, Kenya Red Cross, Liverpool VCT, Land O'Lakes, PATH, Christian Health Association of Kenya, ICAP, Government of Kenya Ministries of Health, and Kenyan faith-based and communitybased organizations USAID Contact: Dr. Stanley Bii, Activity Manager USAID/Kenya Tel: +254-20-862-2211 Email: firstname.lastname@example.com
Cecily with her HIV-free son Ian - Cecily and her husband used their knowledge of prevention of mother-to-child transmission when they decided to have another child.
APHIAplus, Kamili Contact: Dr Mildred Mudany, Chief of Party, APHIAplus Kamili Tel: +254 20 3751882 Email: firstname.lastname@example.com Updated March 2014


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Service age groups
This topic has been updated with 2016 Census data for the LGA or other primary area only. What's this?
Sandringham had a higher proportion of pre-schoolers and a higher proportion of persons at post retirement age than City of Bayside in 2011.
The Age Structure of Sandringham provides key insights into the level of demand for age based services and facilities such as child care. It is an indicator of Sandringham's residential role and function and how it is likely to change in the future.
Service age groups divide the population into age categories that reflect typical life-stages. They indicate the level of demand for services that target people at different stages in life and how that demand is changing.
To get a more complete picture Sandringham's Age Structure should be viewed in conjunction with Household Types and Dwelling Types.
Derived from the Census question:
'What is the person's date of birth or age?'
Total population
Source: Australian Bureau of Statistics, Census of Population and Housing (opens a new window) 2006 and 2011. Compiled and presented by .id (opens a new window), the population experts.
(Usual residence data)
Compiled and presented in profile.id by .id, the population experts.
Compiled and presented in profile.id by .id, the population experts.
Dominant groups
Analysis of the service age groups of Sandringham in 2011 compared to City of Bayside shows that there was a similar proportion of people in the younger age groups (0 to 17 years) and a higher proportion of people in the older age groups (60+ years).
Overall, 23.7% of the population was aged between 0 and 17, and 25.4% were aged 60 years and over, compared with 23.5% and 23.9% respectively for City of Bayside.
The major differences between the age structure of Sandringham and City of Bayside were:
- A larger percentage of 'Frail aged' (5.9% compared to 3.6%)
- A smaller percentage of 'Older workers & pre-retirees' (13.1% compared to 14.2%)
Emerging groups
From 2006 to 2011, Sandringham's population increased by 417 people (4.8%). This represents an average annual population change of 0.94% per year over the period.
The largest changes in the age structure in this area between 2006 and 2011 were in the age groups:
- Empty nesters and retirees (60 to 69) (+190 people)
- Tertiary education and independence (18 to 24) (+148 people)
- Young workforce (25 to 34) (-132 people)
- Parents and homebuilders (35 to 49) (+125 people)


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Although many drugs inhibit the replication of Epstein-Barr virus (EBV) in cell culture systems, there is still no drug that is effective and approved for use in primary EBV infection. More recently, maribavir (MBV), an l-ribofuranoside benzimidazole, has been shown to be a potent and nontoxic inhibitor of EBV replication and to have a mode of action quite distinct from that of acyclic nucleoside analogs such as acyclovir (ACV) that is based primarily on MBV's ability to block the phosphorylation of target proteins by EBV and human cytomegalovirus protein kinases. However, since the antiviral mechanisms of the drug are complex, we have carried out a comprehensive analysis of the effects of MBV on the RNA expression levels of all EBV genes with a quantitative real-time reverse transcription-PCR-based array. We show that in comparisons with ACV, the RNA expression profiles produced by the two drugs are entirely different, with MBV causing a pronounced inhibition of multiple viral mRNAs and with ACV causing virtually none. The results emphasize the different modes of action of the two drugs and suggest that the action of MBV may be linked to indirect effects on the transcription of EBV genes through the interaction of BGLF4 with multiple viral proteins.
After nearly three decades of study, there is still no drug approved for use in primary Epstein-Barr virus (EBV) infection despite the fact that many drugs inhibit the replication of this virus in cell culture, in part because of the complex pathogenesis of infectious mononucleosis (16). The first drug shown to inhibit EBV replication was phosphonoacetic acid (19, 35, 57), but the most intensively studied has been the acyclic nucleoside analog acyclovir (ACV) (8, 10, 39, 48). More recently, 1-H-β-l-ribofuranoside-2-isopropylamino-5,6-dichlorobenzimidazole(maribavir [MBV], also known as benzimidavir), which represents a new class of antiviral drugs, has been extensively examined. While other antiviral drugs inhibit multiple members of the herpesvirus family, MBV inhibits the replication of only human cytomegalovirus (HCMV), a member of the betaherpesvirus subfamily, and EBV, a member of the gammaherpesvirus subfamily (3, 64, 68). ACV is an effective inhibitor of several herpesviruses but not HCMV; ganciclovir, another acyclic nucleoside analog, is a potent inhibitor of HCMV and EBV replication, but its use is restricted because of significant toxicity (26). There is, therefore, a strong interest in MBV because of its favorable toxicity profile as well as efficacy (32, 63). Moreover, since EBV as well as HCMV are reactivated and produce disease in immunosuppressed patients such as bone marrow and organ transplant recipients (38, 55), MBV is a promising agent with a dual potential.
The potency of MBV against HCMV is equal to or better than that of ganciclovir or phosphonoformic acid (Foscarnet). It is much more potent than ACV, and more importantly, it is very active against HCMV strains that are resistant to either of these drugs (42, 64). Therefore, MBV is an excellent candidate to prevent and treat HCMV diseases in high-risk patients based on its efficacy and safety profile in phase 1 clinical trials (32, 63). A phase 2 clinical trial showed that MBV significantly decreased HCMV infection and prevented HCMV disease in allogeneic stem cell transplant recipients. It did so without causing myelosuppression or renal toxicity (65).
Unlike nucleoside analogs, MBV does not target the HCMV DNA polymerase, and unlike its parent compound, 1-H-β-d-ribofuranoside-2-bromo-5,6-dichlorobenzimida-zole (BDCRB), it does not block the cleavage of high-molecular-weight viral DNA concatemers in proviral genomes by the terminase complex, which is composed of UL56 and UL89 (3, 27, 29, 68). Instead, MBV inhibits the protein kinase (PK) activity of UL97 (measured by the autophosphorylation and phosphorylation of histone). Single point mutations in UL97 render HCMV resistant to MBV (2, 3, 6). MBV also blocks HCMV capsid egress from the nucleus and induces highly refractile bodies (aggregates of tegument proteins) in infected cells. As both phenomena are also observed in cells infected with HCMV-UL97 knockout mutant viruses (27, 51), it is likely that MBV interferes with some principal functions of UL97 PK. Many targets of UL97 are probably unknown, although recent data suggest that UL97 can interact with the HCMV pp65 tegument protein as well as cellular proteins such as EF-1delta, among others (21, 24). MBV may also exert its anti-HCMV effects through additional pathways since point mutations of UL27, an HCMV protein without a known function (and not conserved in EBV), also lead to low-level resistance to MBV (7).
Work with its homolog, the EBV PK BGLF4, showed that MBV could affect the essential replication gene, early antigen-diffuse (EA-D [BMRF1], the EBV DNA polymerase processivity factor), by inhibiting its hyperphosphorylation (15, 68). However, as indicated above, the mode of action of the drug is complex, with multiple potential targets, which also include EBV maturation proteins.
EA-D is an early gene and exists in at least three different molecular masses, ranging from 49 to 54 kDa, due to phosphorylation during EBV replication (5, 37, 50). When EBV replication is induced in Akata cells, EA-D is phosphorylated in a temporal fashion and is dominated by the hyperphosphorylated form (54 kDa) at 12 h postinduction (hpi). In addition, PK induces the phosphorylation of a smaller 52-kDa form. The smallest form (49 kDa) is thought of as being nonphosphorylated but is probably phosphorylated to some extent by cellular kinases (9). The two larger sizes were confirmed to be hyperphosphorylated forms based on results of phosphatase treatment (15, 68). In the presence of MBV, only 49-kDa EA-D is detected in induced Akata cells (14, 15). Since UL97 also phosphorylates UL44 (viral DNA polymerase processivity factor for HCMV) (28, 40), and MBV inhibits UL97 kinase activity (3), it is reasonable to hypothesize that the reduced levels of the hyperphosphorylated and hypophosphorylated forms of EA-D produced by MBV are due to the inhibition of BGLF4. However, since MBV was previously reported not to inhibit BGLF4 kinase function directly either in in vitro kinase assays or upon the transient coexpression of the two proteins (14, 15), its effects on EA-D during lytic replication are apparently indirect. Therefore, mechanisms of the anti-EBV activity of MBV and its inhibition of phosphorylated forms of EA-D remain elusive. However, it can be expected that BGLF4 plays diversified yet critical roles in EBV replication and pathogenesis.
In this study we used a different approach to gain a fuller perspective of the complex antiviral mechanisms of MBV by analyzing the expressions of all EBV-specific genes during the lytic program in Akata cells treated with MBV in comparison with ACV. For this purpose, we took advantage of our established quantitative real-time reverse transcription-PCR-based array that is able to measure the viral mRNA levels expressed by all the genes in the EBV genome (31). We show that while the majority of EBV genes are expressed at significant levels after the induction of lytic EBV replication, the EBV RNA expression profiles found with the two drugs are entirely different. The results emphasize the distinct modes of action of MBV and ACV and suggest that the mechanism of action of MBV may be linked to its indirect effects on the transcription of EBV genes through the interaction of BGLF4 with multiple viral proteins (69).
Akata is an EBV-positive cell line derived from Burkitt's lymphoma (gift of Lindsey Hutt-Fletcher). The cells were cultured in RPMI 1640 medium (Mediatech, Inc., Manassas, VA) with 10% fetal calf serum (HyClone, Logan, UT), 2 mM l-glutamine, and antibiotics (culture medium) at 37°C in 5% CO2. For the induction of lytic infection, the number of cells was adjusted to 2 × 106 cells/ml in culture medium, and goat F(ab′)2 fragment to human immunoglobulin G (IgG) (anti-human IgG, 100 μg/ml; MP Biomedicals, LLC, Solon, OH) was used to cross-link cell surface IgG (43, 66). Cells were kept at 37°C for 1 h with occasional mixing before readjusting to 1 × 106 cells/ml. Antiviral drugs were diluted in culture medium and added with anti-human IgG. Culture medium with corresponding concentrations of MBV or ACV was used to readjust cell concentrations. MBV was obtained from GlaxoSmithKline (Research Triangle Park, NC), and ACV was purchased from Sigma-Aldrich (St. Louis, MO).
Akata cells were collected, resuspended in 1 ml Tri reagent (Sigma-Aldrich, St. Louis, MO), and frozen at −80°C before extraction according to the manufacturer's recommendations. Briefly, 200 μl of chloroform was added to tubes containing harvested cells in Tri reagent, vortexed, and transferred into phase-lock tubes (Eppendorf, Brinkman Instruments, NY). After centrifugation, supernatant fluid containing RNA was extracted once with phenol-chloroform-isoamyl alcohol (25:24:1 mixture, pH 5.2; Fisher Scientific, Inc., Pittsburgh, PA) and once with chloroform. RNA was precipitated with an equal volume of isopropanol (Sigma, St. Louis, MO) containing 1 μl of GlycoBlue tracer (Ambion, Inc., Austin, TX) and suspended in 1× Tris-EDTA buffer (10 mM Tris, 1 mM EDTA [pH 7.5]; Fisher Scientific). RNA quality was checked by monitoring of the A260/A280 ratio and by electrophoresis on agarose gels.
The mRNA fraction was enriched with an Oligotex mRNA minikit (Qiagen, Inc., Valencia, CA) according to the manufacturer's protocol except that mRNA was eluted in 0.1× Tris-EDTA (pH 7.5). Reverse transcription was performed with a high-capacity cDNA archive kit (Applied Biosystems, Austin, TX) in a single cycle of a four-step reaction in a thermocycler (Eppendorf AG, Hamburg, Germany). The remaining RNA was digested with RNase H (Epicenter, Madison, WI).
For real-time quantitative PCR (QPCR), 2.5 μl of primer mix was combined with 7.5 μl SYBR green 2× PCR mix (Applied Biosystems) and 5 μl cDNA and subjected to real-time QPCR by using an MJR Opticon2 cycler under standard cycling conditions. Data were normalized to cellular reference genes and analyzed by unsupervised clustering as described previously (49).
For each biological replicate series, the raw threshold cycle values were normalized to the median of four reference genes for each sample to yield the difference in cycle threshold (dCT). These values were clustered by gene with the use of a correlation metric and depicted as heat maps (12).
Statistical analysis was conducted using R, version 2.8.0. First, we analyzed each biological replicate separately.
Based on density plots, we eliminated genes with mean dCT values of <−10 or >10. The resulting mean dCT values were approximately normally distributed (see Fig. S1 in the supplemental material), with a minimum dCT value of −3.5, a maximum dCT value of 12.3, a mean dCT value of 3.7, a median dCT value 3.6, a first-quadrant dCT value of 1.8, and a third-quadrant dCT value of 5.4.
Based on density plots, we eliminated genes with mean dCT values of <−5 or >9.5. The resulting mean dCT values were approximately normally distributed, with a minimum dCT value of −9.7, a maximum dCT value of 15.9, a mean dCT value of 3.6, a median dCT value of 3.1, a first-quadrant dCT value of 1.3, and a third-quadrant dCT value of 5.5 (see Fig. Fig.33).
Two-way analysis of variance was conducted to determine the effect of time and treatment. The P value was adjusted for multiple comparisons using Bonferroni correction. We excluded the zero time points from statistical comparisons, since there was no difference in treatment. Next, we used a generalized linear model to identify the individual genes in the array independently and q value comparison (56) to adjust for multiple comparisons.
Cells were washed once with ice-cold phosphate-buffered saline and deposited onto 14-well 5-mm glass slides to create a cell monolayer (Erie Scientific Co., Portsmouth, NH). Cells were fixed with cold acetone and stained with monoclonal EA-D antibody (Capricorn Products, Inc., Portland, ME), monoclonal gB (gp125) antibody, or monoclonal gp350/250 antibody (Millipore Corp., Billerica, MA). Isothiocyanate-conjugated goat anti-mouse IgG (Santa Cruz Biotechnology, Santa Cruz, CA) was applied, and photography was performed with an Axioskop 2 microscope (Carl Zeiss Microimaging, Inc., Thornwood, NY) at a 200× amplification.
Cells were washed once with ice-cold phosphate-buffered saline and digested in lysis buffer containing 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, 5 mM dithiothreitol, 0.2 mM Na3VaO4, 100 mM NaF, 1 mM phenylmethylsulfonyl fluoride, and protease inhibitors (Roche Diagnostics GmbH, Mannheim, Germany) (15, 68). Protein lysates were separated by electrophoresis on sodium dodecyl sulfate-10% polyacrylamide gels and transferred onto a PVDF-Plus membrane (0.45 μm; GE Water & Process Technologies, Trevose, PA) with a Trans-Blot SD semidry transfer cell (Bio-Rad, Hercules, CA). The membrane was preincubated in blocking buffer of 5% fat-free milk in Tris-buffered saline solution with 0.1% Tween 20 (pH 7.6). The membrane was probed with monoclonal EA-D antibody or monoclonal antibody to β-actin (Sigma) overnight at 4°C and was then probed with horseradish peroxidase-conjugated anti-mouse secondary antibody. Protein levels were visualized with SuperSignal West Pico chemiluminescent substrate (Thermo Scientific, Rockford, IL) and Blue Basic Autorad film (ISC BioExpress, Kaysville, UT).
The cross-linking of B-cell receptors expressed on latently infected Akata cells by anti-human IgG treatment efficiently induces lytic EBV replication. This system has been used to analyze the effects of anti-EBV drugs including ACV, phosphonoacetic acid, and MBV (15, 66, 68). We selected concentrations of MBV and ACV that were sufficient to inhibit EBV replication without significant toxicity, as determined previously. Effects of the drugs on EA-D expression were analyzed by immunoblotting analyses of whole-cell extracts collected 24 hpi, when the three forms of EA-D are at their highest level. EA-D was not detected in uninduced Akata cells, whereas three bands corresponding to molecular masses of 54, 52, and 49 kDa could be detected in induced cells (Fig. (Fig.1A).1A). The 54- and 52-kDa forms represent EA-D hyperphosphorylated and hypophosphorylated by EBV PK; the 49-kDa form is not phosphorylated by viral PK. MBV (20 μM) almost completely inhibited the appearance of the 54-kDa and 52-kDa EA-D products and also reduced the level of 49-kDa EA-D but to a lesser extent and in a dose-dependent manner; in contrast, ACV (50 μM) had no effect on EA-D protein, in agreement with previous results (15, 68). Throughout this study, the concentrations used are ~20 times greater than the 50% inhibitory concentrations (0.3 to 10 μM for ACV and 0.15 to 1.1 μM for MBV) and about five times less than the 50% cytotoxicity concentrations (250 μM for ACV and 94 μM for MBV) (13, 14, 39, 47).
We next examined the effects of MBV and ACV on the expression of the early (EA-D) and the late (gp125 and gp350) viral proteins by immunofluorescence staining. EA-D was detected in the nucleus, while both gp125 and gp350 were detected in the cytoplasm. However, some EA-D could be detected in cytoplasm. On average, 39% ± 5% of cells entered the lytic cycle, as indicated by the detection of EA-D, compared with 2% ± 1% of cells without induction, and 27% ± 3% and 26% + 3% of cells expressed the gp125 and gp350 proteins, respectively (<1% without induction). MBV (20 μM) reduced the number of cells expressing EA-D by about 50% and almost eliminated the expression of gp125 and gp350 (Fig. 1B and C). In contrast, ACV (50 μM) had virtually no effect on the percentage of cells expressing EA-D and inhibited gp125 and gp350 to a lesser extent, as shown by immunostaining and summarized graphically (Fig. 1B and C). There was little effect on cell viability in the presence of either drug at 48 hpi. By 72 hpi viable cells were reduced by about 50%, as expected, after the induction of lytic replication (Fig. (Fig.1D).1D). These findings clearly show the potent inhibitory effect of MBV on the phosphorylation of EA-D during lytic infection and the lack of such an effect produced by ACV. Both drugs inhibited the expression of the two late viral glycoproteins, but MBV had a considerably greater effect, probably due to its greater antiviral potency.
To assess the effect of MBV on EBV transcription, we used EBV-specific real-time QPCR arrays as described previously (31). We conducted two biological-replicate experiments and analyzed the resulting mRNA levels separately by unsupervised hierarchical clustering (Fig. (Fig.2).2). Note that no data were removed due to quality control issues. Results were surprising: MBV treatment resulted in the reduction of EBV mRNA levels, whereas the effect of ACV treatment was negligible (Fig. (Fig.2B).2B). The MBV effect was evident at early time points but most pronounced for certain gamma transcripts: BDLF1, BCLF1, BDLF2, BLRF1, BXRF1, and BBRF3. The reason why the inhibition of these genes stands out is that their RNAs are undetectable in uninduced cells and then accumulate to very high levels, as indicated in Fig. Fig.2B2B.
The raw data were further processed by statistical analysis. First, we analyzed each biological replicate separately. A subset of good dCT data for only the EBV genes and not the reference genes was derived by removing extremely abundant (e.g., BWRF1 repeat) transcripts or those below the limit of detection. As expected, the overall data revealed a striking time dependence of EBV transcription (P < 10−14, adjusted for multiple comparisons) (Fig. (Fig.3A),3A), providing evidence that F(ab)2-IgG treatment of Akata cells induced a coordinated cascade of EBV gene transcription. Figure Figure3A3A shows low and identical levels at 4 h for mock- and MBV-treated cells. Upon induction in the absence of MBV, all EBV mRNAs were induced up to 48 h. By 72 h, the induction of the mRNA was stopped by the effects of viral replication in the cells. In the presence of MBV, overall EBV mRNA levels increased initially (8 h) but not thereafter. The difference between MBV and ACV treatment was significant, with a P value of ≤4.02 × 10−7. There was no statistical difference in overall EBV gene expression levels between ACV and mock treatments, which served as a negative control. These results demonstrate that MBV inhibits EBV transcription.
The effects mirror the first data set (see the supplemental material). The time dependence was significant, with a P value of ≤10−14 (adjusted for multiple comparisons). The time zero sample for mock-treated cells had unusually high levels of housekeeping mRNAs; hence, the mean dCT numbers are lower. This becomes evident in Fig. S1 in the supplemental material but was corrected for in the heat map. Because this first replicate had fewer data points, we did not use it for statistical analysis. In summary, MBV causes an extensive reduction in levels of transcription of EBV mRNAs.
Our statistical analysis derives power from taking into account all time points rather than doing a pairwise comparison, which would in fact omit most of the data. There were no statistically significant differences at any time or for any mRNA between ACV- and mock-treated cells. There were statistically significant differences across all mRNAs and time points in aggregate (Fig. (Fig.3A)3A) between ACV and MBV at a P value of ≤10−7 based on two-way analysis of variance (time and drug).
We then used a linear model to determine for each mRNA individually whether it was differentially regulated between ACV and MBV. We added these differences for each time point and plotted the cumulative differences in Fig. Fig.3B.3B. As can be seen, these differences have a long-tailed distribution (one large peak and three small peaks). The largest peak comprises those mRNAs that are not significantly changed (P ≥ 0.05 after adjustment for multiple comparisons); the smaller peaks on the right in Fig. Fig.3B3B comprise mRNAs that differ significantly with the different drug treatments. We identified the most differentially changed mRNAs by name in Fig. Fig.3B,3B, based on the shape of the density distribution rather than a single fixed cutoff.
The genes that exhibited the largest cumulative differences under MBV compared to ACV treatments across all time points were BDLF1, BDRF1a, BCLF1, EBNA3B, BDLF2, BLLF3, BDLF3, EBNA3C, and BNRF1 (Fig. (Fig.3B).3B). The identities (name and temporal class) and functions, if known, of these genes are shown in Table Table1,1, which shows the changes in mRNA levels relative to levels of uninduced cells (time zero) for each of these viral genes. The changes are the basis for the statistical analyses. For each of the nine genes, mRNA levels increased over time after induction in the absence of drug (labeled mock). Their mRNA levels still increased in the presence of ACV. This increase appears to be less dramatic and delayed upon ACV treatment. However, the differences between ACV- and mock-treated cells were often within twofold and did not rise to the strict level of significance in our statistical analysis. In contrast, MBV inhibited any increase in mRNA levels upon the induction of viral lytic replication, with the exception being BLLF3, where both ACV and MBV seemed to delay mRNA downregulation at late times. This outcome would be expected if the BLLF3 downregulation were mediated by a late viral gene, the accumulation of which is inhibited by either drug. At this time, we do not know the identity of this factor.
In Table Table1,1, we also provide a relative measure of the mean abundance of each of these viral mRNAs vis-à-vis GAPDH (glyceraldehyde-3-phosphate dehydrogenase) levels. This shows that the mRNAs for each of these genes are relatively abundant (7 to 72% for mock-treated cells) and thus within the linear range of our assay. This summary measure also confirms the more detailed comparison based on changes at multiple time points. Overall, ACV does not inhibit mRNA levels by more than twofold, whereas MBV inhibits viral mRNA levels 5- to 40-fold.
Note that this selection reflects both the level of the mRNA and the magnitude of the decrease. These are biomarkers that distinguish MBV from ACV. However, profiling experiments, in general, cannot exclude the possibility that different genes, which change less dramatically in response to treatment, are rate limiting for the replicative cycle and mediate the MBV effect.
Currently available antiviral drugs that might be used to treat EBV infection can be divided into two main classes based on whether they target herpesvirus DNA polymerases or not (14, 38, 47, 55). Acyclic nucleoside and phosphonated nucleotide analogs are incorporated into viral DNA and cause DNA chain termination during viral DNA synthesis. These analogs have much greater affinity for viral than cellular DNA polymerase (10, 47). Pyrophosphate analogs compete with nucleotides in binding to pyrophosphate-binding sites of DNA polymerase. ACV is an acyclic guanosine analog that has consistently been shown to have high anti-EBV activity even though unlike herpes simplex virus (HSV), it cannot be efficiently phosphorylated by the EBV thymidine kinase (BXLF1) (11, 20, 39, 44, 68). Our early work showed that the anti-EBV effect is possibly due to the exquisite sensitivity of EBV DNA polymerase so that even a small amount of triphosphorylated ACV is effective (48).
The second group of drugs includes quite different compounds with distinct structures, foremost among them being MBV and its parent, d-ribonucleoside (BDCRB), each of which has a completely different mode of action (3, 14, 64). MBV was developed in place of BDCRB, the development of which was abandoned since BDCRB is rapidly inactivated metabolically (61). MBV inhibits the replication of EBV and HCMV; the effects on HCMV involve the inhibition of enzymatic activity of the HCMV PK UL97, discovered initially through drug resistance caused by a mutation of the gene. While MBV inhibits the hyperphosphorylation of EA-D, the essential EBV DNA processivity factor, by the EBV PK BGLF4, in cell culture (Fig. (Fig.1A)1A) (4, 15), the drug does not inhibit the enzymatic activity of BGLF4 in vitro (14). Therefore, the inhibitory mechanism of MBV for EBV remains far from being understood.
BGLF4 belongs to the family of conserved herpesvirus PKs, which includes HCMV UL97, HSV UL13, and HSV US3 (17). UL97 and BGLF4 might be expected to have many functions in common, including some carried out by HSV's two kinases. HSV US3 phosphorylates a host of viral and cellular proteins involved in blocking the apoptosis of infected cells (36), and it may be needed for the optimal growth of HSV (52, 53). HSV UL13 is able to phosphorylate several viral and cellular genes, and its biological roles are still under investigation. HSV UL13 phosphorylates US3 (22) and is also required for the optimal growth of HSV (58). Both HCMV UL97 and EBV BGLF4 are essential genes (18, 27, 51). BGLF4 is functionally more similar to HCMV UL97 than to HSV UL13 despite a low degree of amino acid identity (54). It is likely that BGLF4 and UL97 share common functions in viral replication since BGLF4-specific small interfering RNAs inhibit the nuclear egress of EBV capsid (18), similar to the phenotype of cells infected with UL97-deleted HCMV (27).
These two MBV-sensitive viral kinases have more than one target and can phosphorylate cellular in addition to viral proteins. HCMV UL97 phosphorylates cellular elongation factor 1δ (25); histone H2B (2); p32 and nuclear lamins A, B, and C (41); and HCMV pUL44 (28, 40) and pUL69 (60). EBV BGLF4 is able to autophosphorylate and to phosphorylate many exogenous viral substrates such as EBNA-LP (23), EBNA2 (67), and BZLF1 (1) in addition to EA-D (4, 15) and also ganciclovir (Q. Meng, S. Hagemeier, J. S. Pagano, and S. Kenney, unpublished data) as well as cellular targets including elongation factor 1δ (24), condensin, topoisomerase II (33), replication origin-binding protein MCM4 (30), lamin A (34), histone protein H2AX (59), and interferon regulatory factor 3 (62). At present, the significance of the interaction between BGLF4 and these targets for EBV replication is undefined. Moreover, it is very likely that the list of BGLF4 targets will continue to grow, and more functions of BGLF4 will be defined, since another 19 EBV proteins have recently been reported to interact with BGLF4 in protein chip arrays (69), and most if not all of these proteins are phosphorylated by BGLF4. However, whether MBV blocks the functions of these substrates is largely unknown.
Our results indicate that MBV inhibits the phosphorylation of EA-D; reduces the protein expression of EA-D, gp125, and gp350; and inhibits EBV transcription in induced Akata cells. It is unclear why some EBV transcripts are downregulated to a greater extent than others. Many of the most highly downregulated transcripts are adjacent to each other in the EBV genome and may be under the control of the same promoter. No known EBV transcription factors were found to be downregulated. However, cellular transcription factors are involved in the regulation of transcription of many EBV genes and may be a target for MBV. MBV treatment also results in reduced EA-D phosphorylation, a known target for the EBV PK, suggesting that BGLF4 may be inhibited by the drug and is involved in decreased EBV transcription. Similar to the effects of MBV treatment, EBV mutant virus in which EA-D is knocked out does not express the late viral gp125 and gp350 genes (46). In contrast, the knockout of BGLF4 has almost no effect on the expression of EBV early and late genes including gp125 (45). Future work is designed to address the interaction of MBV and BGLF4 and to evaluate the mechanisms through which MBV downregulates viral transcripts.
We thank Lindsey Hutt-Fletcher for Akata cell lines and technical advice and Anuradha Gullapalli for technical assistance with immunofluorescence staining.
This work was supported by funding from the Leukemia and Lymphoma Foundation (grant 6021) and NIH grants DE018304 to D.P.D. and HL64851-06 to J.S.P.
Published ahead of print on 16 September 2009.
†Supplemental material for this article may be found at http://jvi.asm.org/.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text: Hi Ive 'lurked' on this forum for a bit,but now Ill start posting! Id just like to know is there anyone here from Ireland and if there is would they be interested in meeting up for a drifting session somewhere? Im based in Dublin and have a Yokomo HKS S15,although that figure might be 2 soon (I hope!) Also are there any events in the UK as I might be willing to travel! Ed


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The Shakoki Dogu was a powerful spirit inhabiting and guarding the Twilight Mountains. [1]
Creation Edit
When the First Oni was mortally wounded by the Kami Shiba it travelled to the Twilight Mountains before it died. Its foul blood tainted the ground and awakened the Shakoki Dogu, whose duties became to contain the evil of the First Oni and prevent it from returning to the mortal realm. [2]
Demeanor Edit
Shakoki Dogu possessed an intellect and lived only to protect, but it did so in a manner of its own choosing. The Shakoki Dogu did not understand human thought, behavior, or physiology and sometimes its gestures could go tragically wrong. [1]
Boar Clan Edit
The Shakoki Dogu grew lonely, and when a force of Crab entered the Twilight Mountains the Shakoki Dogu imprisoned them. Sixty years later the Crab finally convinced the Shakoki Dogu to release them, and in return they would always guard the Twilight Mountains alongside the Shakoki Dogu. The returned Crab mined the mountains and the wealth earned them the right to found a minor clan, the Boar Clan. [2]
Yajinden and Ryuden Edit
The wealth that emerged from the Twilight Mountains drew the attention of Asahina Yajinden and Agasha Ryuden. The two travelled to the Boar Clan and used the tainted ore to create the Anvil of Despair. Yajinden began slaughtering the Boar Clan on the Anvil, and the Shakoki Dogu could not prevent him. The best they could do was to gather the surviving Boar and take them back to their spirit realm. [2] In its zeal to protect the Boar, it tore them from the mortal realm again, permanently transforming the entire Minor Clan into flesh-less spirits. [3]
Heichi Jianzhen Edit
With the arrival of Kokujin in Shiro Heichi in 1159, the Shakoki Dogu allowed Heichi Jianzhen to return to the mortal realm in hopes that she could uncover why they could no longer enter Shiro Heichi. [2]
First Oni Edit
Kokujin unleashed the First Oni against a group of Dragon heroes, shaken the earth and provoking lava streams. When the Anvil of Despair fell in the lava the Shakoki Dogu were able to stop the summoning of the Oni in the mortal Realm. [4]
Effigies and Stone Circles Edit
There were shrines dedicated to the Shakoki Dogu, in hopes that it would show mercy should it ever re-awaken. [5] Near these mysterious stone circles created by the spirit could frequently be found the Shakoki Dogu Effigies, tiny stone dolls with deformed limbs and wide staring eyes. These dolls were formed from the mountain rock and possessed a shred of the Shakoki Dogu's awareness. They were the spirit's attempt to understand the mortal realm, a reflection of how it perceived humanity. When the Shakoki Dogu wished, it could animate these magical effigies. The effigies never spoke, except to occasionally whispered the Shakoki Dogu's name. For this reason, many explorers had assumed that dolls were Shakoki Dogu. [6] The only known writings on these spirits were found in a small shrine part of a large complex known as Priests' Point, in the edge of the secluded and secretive last stronghold of the Boar Clan, the Lair. [7]
Awakening Edit
In 1132, disturbed by the growing power of the Lying Darkness, the spirits of the Shakoki Dogu within a circle of standing stones near the village of Hoshino began to awaken. The once featureless boulders, were gaining a discernable shape. Many villagers were plagued by chaotic nightmares, caused by the Shakoki Dogu, who needed power to reawaken fully, and they took it from living beings. A group of samurai intervened and stopped their awakening. [8]
External Links Edit
- Shakoki Dogu (Ambition's Debt)
|This Boar Clan related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


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Trump: Oil Can Flow to Mexico 'Right Under the Wall'Announcing a new pipeline between the two countries, he said: "We have to dig down a little deeper under that section."
Nigeria's Oil Militants: 'We'll Give Peace A Chance'Nigeria slipped into recession in 2016 after militants blew up oil facilities. But the government is trying to negotiate peace.
Nigeria's Oil-Producing Delta is Calm—But for How Long?While a fragile truce is allowing Nigeria to step up oil output, a resolution of the crisis is unlikely without further concessions.
Oil Prices Plunge With U.S. Production SoaringOil companies' cost-cutting efforts virtually ensure we won't soon see a return to $100 per barrel pricing.
Shedding Foreign Oil Will Require Higher Gas PricesThe hard truth is OPEC has a lot more power in the oil industry than the U.S. president, no matter who the president is.
Ruptured Pipeline Could Cause Massive Fuel ShortagesAlabama, Georgia, and North Carolina have declared states of emergency due to 338,000 gallons worth of fuel spilling.
House Republicans Are Blocking Exxon InvestigationThe fight to hold ExxonMobil responsible for its climate change denial keeps stalling.
Iraq Considers Selling Oil Through IranKurds are said to be ready to strike an agreement with Baghdad if it guarantees them monthly revenue of $1 billion.
Elon Musk Has a Brilliant Plan to Kill Big OilTesla cars will be a part of a sustainable electric system: the solar panels, the batteries, and the software to manage power and trade it over networks.
Could an Independent Scotland Survive Low Oil Prices?Scotland's fiscal deficit is much worse than the U.K. as a whole.
How Will Turkey's Crisis Affect Oil and Gas Supplies?Further unrest in Turkey could destabilize its pivotal position as a transit state for oil and gas.
Why Oil Was a Prime Motivator For the Iraq War'Oil is part of a general strategy to maintain and exercise global power, and it's a central part of the U.S.-centered global system.'
Saudi Economic Reforms Get Blessing of Top Ministers'Bold' plans to end kingdom's addiction to oil include sales of state assets, private sector investment and more.
Will Donald Trump Take On OPEC?Two energy advisers to Donald Trump want him to call out OPEC members for driving down world oil prices.
Low Oil Prices Exacerbate Iraq's Political CrisisIraqi politicians and energy executives will be in London this week to attend the CWC Iraq Petroleum conference.
Quora: Electric Cars' Impact on Oil-Rich Nations?It may take years, but the effects of the electric vehicle industry will be devastating for many countries.
Oil, Gas Industry Needs 'Intervention' to Save WorkersIn 2014, deaths in oil and gas workplaces made up 79 percent of overall mining industry fatalities, according to the AFL-CIO.
Saudi Arabia Will Struggle to Kick Its Oil AddictionSharing oil wealth in exchange for popular submission is a central part of Saudi Arabia's modern history.
Saudi Prince Urges Reforms to End 'Addiction' to OilPlans include partial privatization of state oil giant and increased roles for women in Saudi Arabian society.
BP Boss Bob Dudley Faces Shareholder ActionSome investors plan to vote against the chief executive's 20 percent pay raise.
Obama to Limit Oil Drilling in Arctic and AtlanticThe five-year drilling plan will work to prevent oil spills and reduce climate change, despite the efforts of major corporations.
Nigeria's Loss-Making Oil Corporation to Break UpDespite improvements, the Nigerian National Petroleum Corporation still loses $15 million per month.
Nigeria and Saudi Arabia to Fight Oil Slump, TerrorismNigeria is fighting Boko Haram while Saudi Arabia recently set up an international coalition against terrorism.
ISIS Is Struggling to Pay Fighters and the Bills: APBills must be paid in U.S. dollars and bonuses have been slashed, according to an AP report.
Don't Panic About the Swings in Oil PricesFor every $70 plunge in a barrel of oil, there's an estimated 1 percent increase in global economic growth.
Nigeria Seeks Billions in Loans to Fund Budget: ReportsThe African nation's budget deficit is predicted to double in 2016.
A Triple Whammy Is Causing Stock Market JittersChina's devaluation, sinking oil prices and Fed monetary policy are rattling investors.
Is It Time to Switch from Riyadh to Tehran?Today it's hard to make a case that petroleum warrants Washington's "special relationship" with Saudi Arabia.
Israel Says ISIS Funded With Turkish Oil MoneyRussia has made similar claims about Turkey, which both Ankara and the U.S. have denied.
The Oil Price Collapse: Bad for Putin, Good for UkraineUnder the circumstances, the Kremlin is unlikely to continue its costly war with Ukraine.


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Ultrabooks have been a hot topic at the International Consumer Electronics Show, but not far behind has been the continued push of tablets.
One of the more interesting tablets on the show floor is the Sony Tablet P, a foldable device that features dual 5.5-inch touchscreens.
When folded, it appears to be easy to pop into a jacket pocket and carry around. It weighs less than a pound, and is slightly more than an inch thick when closed.
Sony says the dual screens bring unique advantages when functioning together. For example, users can read e-mail on the top screen while typing on a virtual keyboard on the bottom. It also seems to be a great fit for fans of electronic books.
MORE: CES coverage
The 4-G tablet runs on Google's Android operating system and features a suite of Sony services such as Video Unlimited and Reader Store. It's also PlayStation Certified, so users will have access to a broad range of classic games.
The Tablet P is an interesting device, as long as Sony can get plenty of developers to tailor their apps for the dual-screen experience.
Sony says price and launch details are to be determined.
Technology Live covers computers, electronics, and the Internet, with a focus on cutting-edge technology that has a real impact on your life. Our reporters and editors -- based in San Francisco, Los Angeles, Seattle, New York, and Washington – offer news and tips to help you make the most of your digital domain. More about the team
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text:
Marc Siegel, Spa Developer, NFS AMERICAS, LLC, Programing
Presently, the Nautilus Brand Platform created by Marc Siegel, spa developer, with all Agreements and work initiated on prototypes, is on the threshold of great success. With that said, NFS has been afforded the following salient elements of significant business value pursuant to contractual agreements, licenses,exclusivity rights and unique opportunities:
Ø A 10 year with a 10 year option exclusive BRAND PLATFORM DEVELOPMENT AGREEMENT with IHG for NFS AMERICAS, led by Marc Siegel, to be their Fitness/Spa/Wellness Consultant in the Americas and create the FITNESS by NAUTILUS - NAUTILUS FITNESS and SPA BRAND PLATFORM.
Ø An exclusive Licensing Agreement for 10 years with TNG, negotiated by Marc Siegel, for their trademarks, logos, intellectual property, equipment, accessories, clothing and name use in the Hospitality Industry.
Ø Execution of an "In-Room Equipment and Exercise Program" within HI and then other divisions, featuring the new TNG TreadClimber, which has the latest patented technology and is considered the most advanced innovation in Cardio Equipment in 2004.
Ø The formation of an exclusive "Private Label – Logo Line" designed by Marc Siegel with NFS AMERICAS to include but not be limited to Apparel, Face and Body Products, Accessories, Water, Juices and Nutritional Bars for exclusive use in all facility and hotel retail-outlets.
Ø TNG's TreadClimber and BowFlex will be included as offerings from IHG's Priority Club, the industry's largest loyalty reward program with over 17 million members.
This programing never reached fruition due to issues within TNG and HI.


text:
Today, liberal members of the House of Representatives decided to stage a sit in to push for gun control legislation. During their free time sitting on the floor they should have done an internet search for "Chicago Deaths This Year." Upon hitting the enter button they would see article after article showing how "well" gun control in this city is working.
Chicago has some of the strictest gun laws in the country. If gun control actually worked, Chicago would be the safest place to live. Instead, this city has reached its 300th homicide this year on Father's Day weekend. From January 1, 2016 to today Chicago has had 312 homicides and 1,803 shooting victims. By the end of 2016, this number will far surpass the 468 homicides and 2,988 shooting victims there was for all of 2015.
Chicago is a wonderful example of a liberally controlled, Utopian project gone wrong. Gun control laws strip law abiding citizens of their constitutionally protected right to defend themselves. Background checks and wait periods won't stop criminals from illegally obtaining the firearms that increase this homicide statistic every day.
To see more stories like this, sign up below for Dennis Michael Lynch's email newsletter.
Sign up to get breaking news alerts from Dennis Michael Lynch.
Muslim Police Officer Refuses to Shave Beard, Is Suspended


text:
Engadget Summary
With so many options available to customers looking for a Samsung tablet -- from the stylus-equipped Galaxy Note 8.0 to the simply stellar Galaxy Tab S -- it takes a lot to stand out from the crowd. The Galaxy Tab4 8.0 is a serviceable slate, but lacks the pizzazz to justify a purchase in lieu of one of its flashier relatives.
While CNET liked the "nice, compact size" of the Tab4, they found the plastic construction "a bit cheap," and PC Advisor noted it "lacks a premium finish." However, CNET says it offers a textured finish that is both "simultaneously smooth and grippy," and HotHardware thinks it's "an improvement" over the glossy casing of the Galaxy Tab 3. The screen is also a bit of a mixed bag -- not quite bad, but also not very flashy. HotHardware says it offers "decent color and viewing angles" but in the end it's only "passable" given that many competing tablets offer higher resolution displays at the same price.
On the inside ChipChick says it can "do pretty much anything your standard smartphone can do," and even PC Mag enjoyed features like the split-screen multitasking. One thing they didn't enjoy was the battery life, with the Tab4 clocking in at around six hours -- a "disappointing" result given the Tab4's lower end specs. But ultimately battery life depends on the user, and ChipChick found the slate lasted two weeks.
The Samsung Galaxy Tab4 8.0 is a nice tablet, but not much more than that. For a few more dollars you could snag yourself a Galaxy Note 8.0, which has a ton more features, even if you never use the S-Pen.


text:
How to Migrate from Microsoft Outlook 2003 to Outlook 2007
Need help making the switch from Outlook 2003 to 2007? Give this guide a gander. This free video tutorial presents an easy-to-follow overview of everything you'll need to know to migrate your data from one program to the other. For more information, and to get started using Microsoft Outlook 2007, watch this free video guide.


text:
Would you like to save as your currency permanently or for this visit only?
The staff were very helpful and friendly. Breakfast was excellent. The meals available in the evening were excellent they lack the important ingredient, for me, of a pudding, but I think and hope that this is in the process of being rectified. The bass of the music played in the bar resonated throught the bedroom, although they did try very hard to keep it down.
Location, friendly staff & value. Timed light in corridor outside room permanently on.
Friendliness & helpfulness of staff; excellent location for riverside & town centre; good food & drink; pleasant atmosphere in bar. Bathroom door didn't close properly; no detachable shower-head; shower leaked onto bathroom floor; insufficient hanging space for clothes
Friendly staff, good location None
Staff were exceptionally friendly & helpfull None
The room was clean and nicely furnished with lovely view of the estuaty,the staff were really friendly and the breakfast was fab! We ate in the restaurant on both nights of our stay and it was really good value for money. We're already planning to visit again in February. When we visit again we'd like to stay in a room with a bath, on the top floor if possible.
Location on banks of River Dart. Excellent breakfast, with swift service. Clean modern bathroom. Bar lacked atmosphere.
Dartmouth is a spectacular gem of a town, off the beaten path and full of genuine charm, especially for anyone who loves the water. This Inn is well-located, the price was right, and the owners have done an excellent job bringing a very old building up to speed. Our room was above the bar and therefore sometimes noisy. The room was quite small but the bathroom was first class.
superb view,near to amenities and friendliness none
Evening food very good Did not enjoy the cooked breakfast - eggs not cooked properly
Our 3rd visit; a cosy.welcoming and friendly place. Comfortable rooms, good food & a good "pubby" feel to the bar
Location, food Sound insulation in room to adjoining guest room not so good
Everthing No
I loved it's warm and worn old English feel and historic charm.
A truly lovely place to stay
would have liked a friendlier welcome, also, being shown to the room would have been a nice touch , as we went to the wrong floor initially, carrying suitcases up and down narrow flights of stairs was a pain !
The comfort of our room was excellent but our friends said their bed had dips in each side and appeared old like the establishment. Thought the dining room for breakfast a little on the dismal side - could of done with more lighting
Absolutely everything!!! (and no I am not connected to the hotel..)
We loved the wonderful food they have a very talented Chef. The staff were friendly and very helpful. The hotel has a nice warm welcoming atmosphere. Our room was disappointing, we felt that it needed some TLC and updating, it felt like a basic over flow room - simple things could have been done: - it would have been helpful to have placed a mirror on the wall or on the chest of drawers near a socket for when drying ones hair. An extra bedside table lamp would also have been helpful lighting was poor it meant that only my husband was able to see to read in bed
Helpful staff. Excellent food. A classy interesting hotel. We did not see any of the resident ghosts!!!


text:
Gift Premium Subscription To smokinbunta
Join Date:03-18-2011
Last Activity:12-07-2011 7:37 PM
- Kansas city burbs,KS
- cars, motorcycles, now guns too
- restaurant
Posted in thread: Thinking about being a Volunteer officer on 06-01-2011 at 10:50 PM
I am not a LEO but I do see that kind of job as a honorable and at times a rewarding one.
However, I am not sure that I would spend my free time being exposed to violence-element of
danger/possible in...
Posted in thread: Khaki Backpack on 05-13-2011 at 06:47 PM
Sorry to bring this back.:oWhat do you guys think about this one?Amazon.com: MEDIUM Molle
Hydration Backpack--TAN: Sports & Outdoorsthat looks nice! now i wish i had saw this before i
bought my maxped...
Posted in thread: Wife got her CHL! Now where to carry? on 05-11-2011 at 10:01 PM
That gives a whole new meaning to booby trap. Just think of all the fun you could have
searching her to find the pistol."is that a gun or r u just happy to see me?" LOL
Posted in thread: 45 cal. Ammo - Sources of Bulk Ammo for Practice? on 05-10-2011 at 09:30 PM
Ammo Deals | Slickgunsthis has pretty good deals... except call the place that is having the
deal first and find out if they actually still have it in stock.. they run out QUICK! or just
go to USAAMMO...
Posted in thread: Stupid things you've seen or done at the range....... on 05-10-2011 at 09:06 PM
Originally Posted by AusLach Not at the range but while out shooting... My brothers and I went
for a drive in the ute (what the hell is a ute?)with my .223 and the .25-06 to get some roos
for dog meat...
Posted in thread: Personal Quirks & Little Habits on 05-08-2011 at 09:46 PM
hum.... i count.... alot... everything from steps to how many times i brush each portion of my
teeth... not that i have to do an exact number every time, but i just count...and not that i
necessarily ...
Posted in thread: Thinking about being a Volunteer officer on 05-08-2011 at 04:50 PM
Thanks for the encouragement! now i was just wondering. is there such thing as too old to go
into the field... im 25 now, and i was planing to make this happen in the next 2-3 years.. 28
isn't too old...
Posted in thread: Full Tang Combat Bolo KUKHRI Knife on 04-29-2011 at 10:19 PM
i knew this looked familiar before i started reading! ima get me 1!!
Posted in thread: WOOHOO! got a SuperNova! on 04-29-2011 at 07:46 PM
THANKS A BUNCH!! that is perfect!
Posted in thread: WOOHOO! got a SuperNova! on 04-29-2011 at 06:49 AM
Ok... Now.. This is my first shotgun n after a better look after getting home. I found that
theres a cap in the mag tube.. Now I went on benellis site n they have pdf manuals n I didnt
see 1 for the s...


text:
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