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Spiral 'Ruffled' Scarf (knit) by Lion Brand Yarn
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by Brackenridge Flickr
Spiral 'Ruffled' Scarf (knit)
This pattern is available for free: kmm-spiralScarf.pdf
This curly 'potato chip' scarf makes up quickly. (Lion Brand Yarn)
Lion Brand Pattern #: 50193-1K
SKILL LEVEL: Easy
(A) Homespun® (Article #790) 1 ball
(B) Moonlight Mohair (Article #510) 1 ball
Knitting Needles size 10 [6 mm] 36 inches [91 cm] or longer circular
Scarf is worked lengthwise. Finished in 11 rows.
Rows 1-10 are worked in (A).
Row 11 (edging) is worked in (B).
Pattern also available from Michaels site.
See also Spiral 'Ruffled' Scarf (crochet) for the crochet version.


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The Future, Today. Aka We're Already Finished
Ticket sales are currently not available for this venue.
The Future, Today. Aka We're Already Finished
It's not the year 2245, but it might as well be. Though we don't have self-lacing shoes or hover-boards, things are changing - with or without us. The cast of THE FUTURE, TODAY invites you to contemplate life on the moon, travel forward in time, and see what the future holds. So look up, before we're already finished.
Written and Preformed by: Stephanie Weber, Carmen Christopher, Eli Weatherby, Emily FitzPatrick, Peter Corey, Jessie Stegner
Musical Direction by: Nick Johnson
Directed By: Joey Lesiak
Fridays at 9pm
The show runs July. 5th-August 2nd.
de Maat Studio Theatre
1608 N. Wells St. - 3rd Floor
The Second City Training Center
Tickets: General $12 • Students $10 • SCTC Students $6
Reserve tickets at www.secondcity.com or
Box Office phone # 312.337.3992

subset: commoncrawl


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Hair goes done iii several stages of ontogenesis. The name calling of these three stages are; anagen, catagen and telogen.
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Would I be a good candidate for small mid face lift? I want to achieve a taut and tight looking face. (photo)
Doctor Answers 16
Facelift: Am I a good candidate for midface lift?
Facelift Probably Not Necessary
Learn more: http://www.visageclinic.com/cosmetic-surgery/facelift.cfm
Ood candidate for small mid face lift? I want to achieve a taut and tight looking face. (photo)
You might also like...
Midface improvement
Hi and thanks for posting your question and photos on RealSelf!!Based on the limited information I can gather from the picture you posted, you appear to be young with good skin and minimal laxity. Your concerns may very well be improved with a combination of skin resurfacing and fat grafting. This would allow for improvement of volume in areas that you desire it with your own fat harvested from areas where you have a bit extra.
I would highly recommend seeing a reputable, board certified, plastic surgeon in your area to discuss your options.
Very best wishes in reaching your goals!
Answer: Face Lift Candidate
Thank you for your question! It is difficult to determine if you are a "good candidate" for a face lift without full assessment of your anatomical features. My best advice would be to schedule a consultation with a board-certified facial plastic surgeon to discuss your main concerns, goals, and realistic outcomes of suggested procedures and treatment options (if any). I hope this is helpful to you!
Be well,
Dr. Todd Hobgood, MDtoddhobgood.com
I want to achieve a taut and tight looking face. (
A taut and tight looking face is almost never a natural looking face. As the tightness from the last procedure stretches away you will want another only increasing the unnatural look to the face and so on. You can chase it forever and never get perfect tightness. You will however achieve a perfect strange look.
I think you look OK myself. I'm not sure where I would fill to make you look better.
Learn more: http://vallambros.com/about.html
Midface lift
Learn more: http://www.facialinstitute.com
Would I be a good candidate for small mid face lift? I want to achieve a taut and tight looking face.
Following the beauty principles outlined in my book on face and body beauty, women look the most feminine, youthful and attractive with heart shaped faces. Heart shaped faces have cheeks that are full and round in the front. Cheek augmentation with a dermal filler or using cheek implants for a permanent enhancement will create full, round cheeks that will feminize the entire face.
If the chin is weak, this creates an imbalance making the nose appear larger, the mid face top heavy and the lower face look short that de-emphasizes the lips and allows early formation of a double chin. Chin augmentation using a chin implant will add projection to the chin creating harmony and balance to the lower face. I have found that placement of a silastic chin implant, through a small curved incision under the chin (also allows excess skin removal) to be very safe, quick and highly effective.
Since you don't have "jowls" which are sagging facial tissues and an indication for some form of a SMAS facelift, this wouldn't be warranted. (I have included information below as a reference.) The underlying SMAS layer, of the face, must be dissected, lifted, trimmed and re-sutured (not merely folded or suspended with threads or sutures that will not last). The excess skin is then removed and the facelift incisions closed.
My most popular facelift is the minimally invasive, short incision facelift that has all the benefits of more invasive facelifts (traditional, mid-face, deep plane, cheek lift and subperiosteal facelifts) but with these added benefits:
- very small incisions
- minimal tissue dissection = less bruising and swelling = rapid recovery
- can be performed in 90 minutes or less, with or without general anesthesia
- no incisions within the hair = no hair loss
- excess fat can be removed
- excess skin removed
- cheeks, chin and jaw line can be augmented with dermal fillers (I prefer Restylane Lyft) or facial implants
- most patients fly back home to parts all over the world in as little as 3 days post-op
Hope this helps.
Learn more: http://www.beverlyhills-plasticsurgery.com/facelift/#sub
Would I be a good candidate for small mid face lift? I want to achieve a taut and tight looking face.
Learn more: http://www.bostonplasticsurgeryspecialists.com/services/face/facelift/
See a board certified surgeon
You really need to see a qualified plastic surgeon in person to determine if you are a good candidate for surgery. We cannot always tell from a picture because a facelift will include muscle work, as well as a determination of skin laxity, among other criteria. That said, I bet you will find surgeons will suggest filler for you.
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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Jeweler to royalty sought the help of Princess in Chiang Mai. Men identify Winston with cigarettes but women think of Diamonds. Back when Myanmar was Burma, and its borders were closed to the outside world, the late Harry Winston, jeweler to royalty and Hollywood stars, visited Chiang Mai to seek advice on Burmese jade from city jeweller Somchai Srisawat, owner of Princess Jewelry.
"He didn't buy anything," Khun Somchai told me, "but he examined my stock, and asked many questions about how to recognise the very best quality of the Burmese stones. "Back then," said Khun Somchai, "I used to run jade auctions in the city; something like Sotheby's or Christie's do today."
Harry Winston's expertise in the diamond trade was second to none, but the multimillionaire asked jade dealers whom to consult on the "Inscrutable gem." Old Harry was advised to head for Chiang Mai to seek out Khun Somchai Srisawat of Princess Jewelry on Changklan Road. I look around for the sign saying, "Consultants to Harry Winston", but see none. I am not an advertising man, but when Khun Somchai reveals that 80% of his customers are from the USA, I urge him to go, with some urgency, to the nearest sign maker!
He shrugs his shoulders, and with a smile as inscrutable as the gemstone in question, hands me over to his lovely daughter, and General Manager of the company, Ann.
Ann is one of three of the five Srisawat children to have gone into the family business, is a fully qualified gemologist , graduate from the world renown Gemological Institute of America ( my translator for the day, since Khun Somchai's English matches my Thai), and a young lady with a flawless command of the English language. She tells me that the most popular items with her clientele are diamond engagement rings, necklaces and pendants, although many Asian customers still buy jade. As we chat, Ann's sister, Sirimas, who graduated in gemology in Belgium, is attending to a customer's needs.
The range is astonishing: from 100 baht trinkets for teenagers to Burmese blue sapphires running into seven figures. Ann tells me that a customer from Taiwan bought one of these stones for six million baht, while the British peer, and television personality Lord Charles Brocket, paid three million baht for his stone.
Ann tells me that her mother and father used to travel abroad to purchase their gems, but have since set up a string of reliable agents in Africa, India, Kashmir and Burma.
She points out that in the company's 34 years of doing business, they have never had one customer complaint, adding that the only time the Tourist Police have been in their shops is as customers! Indeed, a great percentage of Princess Jewelry customers are regulars. People from the United States, Europe and throughout Asia return time and again to make purchases with people they trust.
Ann tells me that her mother, Khun Chongmas, and father, Khun Somchai, do not encourage tour guides or taxi drivers to bring customers to either of their shops on Changklan Road. "We rely on our reputation, alone, to attract customers," says Ann. "We would never entertain touts bringing potential clients to our establishments," she adds.
Thailand has earned the right to be known as the world's gem and jewelry centre, employing more than 1.2 million people with projected export sales of around five billion US dollars before the end of the decade. Precious stones are sent here by some of the world biggest trading houses to be cut and polished by Thai artisans.
Shopping is one of the main reasons people come to Chiang Mai (honestly guys), and if you decide on treating your better half to a ring, necklace, bracelet, or some other special piece of jewelry, you have come to the right place.
Did you know that the first diamond engagement ring was given to Mary of Burgundy from Archduke Maximillian of Austria in 1477? And we men have been led like donkeys by the carat (pun intended) ever since!
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New Student Orientation is much more than just one day of on-campus programming. The Triton Overnight Experience keeps you in the action and lets you experience life as a college student, before you even start classes! Whether you live 3 hours away or right around the corner, the Triton Overnight Experience is a great way to meet new people, learn more about campus life from the orientation leaders and create lasting memories.
The Triton Overnight Experience provides students with overnight housing in Oak Hall Residence Hall and dinner with the Triton Orientation Leaders. After dinner you can hang out with your new friends, participate in fun games and activities or play basketball/sand volleyball with other new students and OLs.
Staying overnight, isn't just about a place to sleep…
it's a Triton Experience you don't want to miss!!!
2017 Dates and Registration
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All students must sign these two waiver documents in order to participate in the Recreation and Wellness Center for our evening activities.
Please email email@example.com if have any questions prior to your NSO and Triton Overnight Experience session date. We look forward to meeting you this summer!


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BORIS/CTCFL is an RNA-binding protein that associates with polysomes
Nuclear function and transport
BORIS (CTCFL), a paralogue of the multifunctional and ubiquitously expressed transcription factor CTCF, is best known for its role in transcriptional regulation. In the nucleus, BORIS is particularly enriched in the nucleolus, a crucial compartment for ribosomal RNA and RNA metabolism. However, little is known about cytoplasmic BORIS, which represents the major pool of BORIS protein.
We show, firstly, that BORIS has a putative nuclear export signal in the C-terminal domain. Furthermore, BORIS associates with mRNA in both neural stem cells and young neurons. The majority of the BORIS-associated transcripts are different in the two cell types. Finally, by using polysome profiling we show that BORIS is associated with actively translating ribosomes.
We have demonstrated the RNA binding properties of cellular BORIS and its association with actively translating ribosomes. We suggest that BORIS is involved in gene expression at both the transcriptional and post-transcriptional levels.
CTCF is a highly conserved and ubiquitous protein that has widespread functions in transcription regulation and chromatin architecture. It acts as a silencing and activating transcriptional factor, a chromatin insulator and a mediator of chromatin looping, and is essential for life
BORIS protein is readily detected in most cells and tissues
We previously showed that BORIS is present both in the cytoplasm and nucleus, and is enriched in the nucleolus, a crucial compartment for ribosomal RNA and RNA metabolism
BORIS is an RNA binding protein
Association of BORIS with newly synthesized RNA was first suggested by a run-on transcription assay on HEK293T cells, which showed that BORIS co-localises with 5-FU in punctate foci in both the nucleus and cytoplasm (Additional file
Additional file 1: Figure S1
Endogenous BORIS co-localises with newly synthesized RNA. HEK293T cells were pulse-labelled with 5'Fuorouridine (5'FU) for 10 minutes to label nascent RNA. Double immunoflorescence was performed with an anti-BrdU antibody to detect 5'FU (Red) and ant-BORIS (Green) as indicated. Nuclei were visualised with DAPI (blue).
Click here for file
Figure 1
Amino acid sequence of BORIS showing predicted nuclear localisation signal (green) and nuclear export signal (blue)
Amino acid sequence of BORIS showing predicted nuclear localisation signal (green) and nuclear export signal (blue). The unstructured N and C terminal regions
We therefore extended our investigation to determine whether BORIS interacts with RNA in other cell types and, if so, whether the interaction changes as cells undergo phenotypic alterations. We previously showed that BORIS is present at similar levels in hNP1 neural progenitor cells (Aruna Biomedical) and young neurons derived from hNP1 using well-defined culture conditions
Additional file 2: Figure S2
Partial PCR amplification of endogenous BORIS in hNP1 cells and HEK293T cells. Gel electrophoresis analysis of fragments used for sequencing amplified with (A) primers +67F and +633R as previously described [22] or (B) with primers BORIS exon 9-10-11 forward: 5'-TGACCGCTCACATTCGTACC-3' and reverse 5'-AGTGAACACGCAACCCGAAT-3'.
Click here for file
To investigate if BORIS associates with endogenous RNA in hNP1 cells and hNP1 cells differentiated to neurons over 6 days (designated 6dN), we used oligo dT beads to precipitate mRNA from cell lysates and analysed co-precipitated proteins by Western Blot. In both cell types, BORIS was precipitated (Figure
Figure 2
Western blot analysis of oligo-dT-precipitated RNA-protein complexes
Western blot analysis of oligo-dT-precipitated RNA-protein complexes. (A) RNA-protein complexes form human neural progenitor cells (hNP1) and hNP1 cells differentiated to neurons over 6 days (6dN) were precipitated with oligo-dT beads analysed by SDS page western blot probed with anti-BORIS antibody (ab18337, Abcam). (B) Immuno-blotting of oligo-dT-RNA bound protein complexes from HEK293T cells transiently expressing BORIS (C3-BORIS) or empty vector (C3-empty). Western blot analysis of Input (2%) and oligo-dT bound protein complex probed with either anti- GFP or anti-BORIS.
We then used native RNA-immunoprecipitation to isolate RNAs that were associated with BORIS. A substantial amount of nucleic acids (5-15% of input) was consistently immunoprecipitated from both hNP1 and 6dN cells. To verify that this was RNA and not contaminating DNA, since BORIS is known to bind DNA
Figure 3
Characterisation of immunoprecipitated nucleic acids
Characterisation of immunoprecipitated nucleic acids. (A) Quantification of BORIS and control IgG immunoprecipitated nucleic acids before and after digestion with either DNase I or RNase A. (B and C) Agilent Bioanalyser gel-electrophoresis of BORIS and control IgG precipitated RNA. The BORIS-precipitated samples revealed a prominent band migrating as 28S rRNA and a less prominent band as 18S in both hNP1 (B) and hNP1 cells differentiated to neurons over 6 days (6dN) (C). Note the different scale of the Y-axis.
Next, to determine whether BORIS binds directly to RNA, a series of 20 mer RNA and DNA homopolymers with 3' Biotin-TEG was utilised in an
Figure 4
BORIS binds directly to RNA in a nucleotide-specific manner
BORIS binds directly to RNA in a nucleotide-specific manner. (A) SDS-PAGE western blot analysis of the purification of Halo tagged BORIS from transfected HEK293T cells. (B)
Identification of poly(A) RNAs bound to BORIS
To identify which transcripts were associated with BORIS in hNP1 and 6dN cells we immunoprecipitated the protein from cellular extracts. We then isolated the RNA and converted it to cDNA, which was hybridized to gene expression arrays. The signals from the arrays were then compared to those obtained from total RNA isolated from hNP1 and 6dN cells. Transcripts were scored as associated with BORIS if the fold change was larger than two and the
Additional file 3: Table S1
Probes and transcripts associated with BORIS in hNP1 cells.
Click here for file
Additional file 4: Table S2
Probes and transcripts associated with BORIS in hNP1 cells differentiated to neurons over 6 days (6dN).
Click here for file
Additional file 5: Table S3
BORIS-associated transcripts up- or down-regulated during neural differentiation.
Click here for file
Characterisation of BORIS-bound transcripts
We first used the PANTHER Protein Class Ontology platform
Figure 5
Panther analysis of transcripts bound to BORIS
Panther analysis of transcripts bound to BORIS. Significantly enriched PANTHER annotations identified for BORIS-associated transcripts. Binomial distribution is applied to obtain
BORIS expression activates the β-catenin dependent WNT canonical pathway
In both hNP1 and 6dN cells, BORIS associates with several transcripts of the WNT pathway, including APC, TCF, lpd5/6, WNT5A and FZD5/10 (frizzled family receptor 5/10) (Additional file
Additional file 6: Figure S4
BORIS associates with RNA transcripts in stem cells and young neurons. BORIS associates with several transcripts (coloured blue) of the WNT signalling pathway.
Click here for file
Figure 6
Activation of the WNT pathway after BORIS over-expression
Activation of the WNT pathway after BORIS over-expression. (A) Western blot analysis of WNT5A/B and TCF3 in untransfected control, mock transfected (C3-empty) or BORIS transfected (C3-BORIS) HEK293T cells. The upper band in the WNT5A/B blot is non-specific (*) as shown by provider (Cell Signalling). The membranes were probed with the antibodies as indicated and GAPDH was used as a loading control. Lysates from three independent experiments were loaded on the same gel. (B) mRNA levels of BORIS bound transcripts in HEK293T cells over-expressing BORIS (C3-BORIS) as compared to empty vector (C3-empty). Data in (B) represent 2 technical replicates ± SD. (C) Luciferase activity of a TCF/LEF-responsive element reporter after BORIS (C3-BORIS) or mock transfection of HEK293T cells. Over-expression of BORIS (C3-BORIS) led to a more than four-fold increase in luciferase activity compared to cells transfected with empty vector alone (C3-empty). Cignal negative is a non-inducible reporter, lacking the TCF/LEF response element. LiCl was used as a general activator of the TCF/LEF reporter. Data are expressed as a mean ratio of the Firefly/Renilla luciferase activity (see Materials and Methods) and represents the average ± SD of 4 biological replicates. (D) Western blot analysis of HEK293T cells after over-expression of BORIS together with siRNA knock down of β-catenin. LiCl treated cells (C3-empty + LiCl) were included in the analysis to exclude a direct effect of LiCl on the expression of β-catenin. GAPDH was used as a loading control. (E) Densitometry quantification of β-catenin expression in (D). Data represents the mean ± SD of 3 biological replicates. (F) Normalised TCF/LEF luciferase reporter activity after β-catenin knock-down and BORIS over-expression in HEK293T cells. β-catenin knock-down efficiently prevented BORIS-mediated activation of the TCF/LEF luciferase reporter, compared to Scrambled + Reporter with β-catenin siRNA + Reporter. Data shown is from one representative experiment.
Additional file 7: Figure S5
Effects on protein levels after BORIS overexpression in HEK293T cells. Images and the associated densitometry measurements used to assess the protein levels of WNT5A/B and TCF3 after BORIS overexpression.
Click here for file
To determine directly if BORIS influences the activation of the WNT pathway, we then used a luciferase reporter assay where the luciferase expression is driven by tandem repeats of multiple copies of the consensus TCF/LEF- β-catenin responsive element (SABiosciences). LiCl, an inhibitor of GSK-3, was used as a positive control for pathway activation
BORIS associates with polysomes
The large amount of RNA including ribosomal RNA, bound to BORIS, suggested that BORIS interacts with the translational machinery. To investigate this directly, we performed polysome profiling on cell extracts prepared from hNP1 and 6dN cells and analysed the distribution of BORIS in the resulting gradients by Western blotting. Consistent with a ribosomal association, BORIS was present throughout the gradient, co-sedimenting with all ribosomal subunits (40S and 60S) as well as monosomes (80S) and polysomes (Figure
Figure 7
Polysome profiling of BORIS
Polysome profiling of BORIS. (A) Polysome profiles of cell lysates from hNP1 and hNP1 cells differentiated to neurons over 6 days (6dN) on a 10-50% sucrose gradient. Lighter particles at the top of the gradient are shown on the left and heavier fractions are shown on the right. Equal volumes of each fraction were analysed by SDS-PAGE and probed with indicated antibodies. S6 and RPL7 are ribosome-associated proteins while GAPDH is not. (B) Polysome profiling of HEK293T cell lysates, detecting endogenous BORIS, after disruption of polysomes by puromycin (top panel), 30 mM EDTA (middle panel) or following RNAse A digestion (lower panel).
Polysome profiling of HEK293T cells showed a similar sedimentation profile of BORIS to that observed in hNP1 and 6dN cells (Figure
Here, we provide evidence that BORIS, best known for its role in DNA binding and transcriptional regulation, also binds RNA
An interesting feature of BORIS is that its mRNA expression is extremely low in cultured or primary cells, yet the protein levels are readily detectable. This is consistent, however, with a report that RNA binding proteins tend to exhibit high protein stability and translational efficiency, yet their transcripts have a short half-life
Although the majority of BORIS-associated transcripts differ between hNP1 and 6dN cells, similarities are observed in the pathways in which the transcripts are involved in the two cell types. For example, BORIS-associated transcripts in both cell types encode proteins involved in the canonical WNT pathway. WNT signalling is crucial in the regulation of a wide range of cellular processes such as apoptosis, cell proliferation, and differentiation, including that of neural stem cells
A role for BORIS in regulating WNT signalling is supported by our finding that BORIS increases the activity of a TCF/LEF reporter following transient over-expression in HEK293T cells (Figure
The biological consequences of the association of BORIS with different transcripts within individual pathways in hNP1 and 6dN cells have yet to be determined. BORIS may be involved in coordinated regulation of different transcripts within certain pathways at specific time points of cell development or differentiation.
We show that BORIS can directly interact with RNA
Cell culture
Human neural stem cells, hNP1, derived from the cell line WA09 (46,XX) (Aruna Biomedical, hNP7013.1), were cultured in Neurobasal medium (Invitrogen, 21103–049) supplemented with B27 (Invitrogen, 12587–010), FGF-2 10 ng/ml, 1% penicillin/streptomycin and 2 mM glutamine as previously reported
BORIS antibody ab18337 (1:1000 dilution, Abcam), CTCF antibody 07–729 (1:1000 dilution, Millipore) and GAPDH antibody 14C10 (1:1000 dilution, Cell Signaling) were used in Western data shown. The specificity of the BORIS antibody was determined using recognition of GFP-tagged recombinant BORIS and non-recognition of GFP-tagged recombinant CTCF protein by western blotting (Additional file
Additional file 8: Figure S3
Confirmation of BORIS antibody specificity. Immuno-blotting of oligo-dT-RNA bound protein complexes from HEK293T cells transiently expressing CTCF (C3-CTCF), BORIS (C3-BORIS) or empty vector (C3-empty). Blot probed with anti BORIS antibodies.
Click here for file
Run-on transcription assay
For immunodetection of newly synthesized RNA, HEK293T cells grown on coverslips were briefly incubated (15–20 minutes at 37°C) with 2 mM 5'-fluorouridine (5-FU) (F5130; Sigma)
Identification of nuclear export signal
Identification of a putative nuclear export signal (NES) in the C terminal region was performed using NetNES (
Oligo-dT precipitation of BORIS
Cells were trypsinised, washed in ice cold buffer A (10 mM Tris–HCl pH 7.5, 1.5 mM MgCl2, 0.2 mM EDTA, 10 mM KCl) and lysed in buffer C (10 mM Tris–HCl (pH 7.5), 100 mM NaCl, 2.5 mM MgCl2, 0.5% Triton X-100, and 2unit/μl RNaseOUT). 1000 μg of protein lysate was incubated with 100 μl oligo-dT-dynabeads (Invitrogen) and incubated at 4°C for 30 minutes. Oligo-dT/mMRNA/protein complex was separated from unbound proteins using an Invitrogen magnetic separator. The beads were washed five times with solution D (20 mM Tris–HCl pH 8.0, 150 mM NaCl, 0.02% NP-40 and 1U/ml RNaseOUT) using at least twice the lysate volume for washing. Beads and attached complexes were re-suspended in 20–40 μl PAGE loading buffer for western blot analysis.
Identification of BORIS bound mRNAs
Immunoprecipitation of BORIS-mRNA complexes was used to assess the association of BORIS with target mRNAs as previously described with some modification
The RNA was used for either hybridization to Affymetrix U133 plus 2.0 expression arrays or for RT-qPCR verification of BORIS target transcripts. For array analysis, double stranded cDNA was synthesized from 1.5 – 5 μg total RNA using the Affymetrix One-cycle cDNA synthesis kit following the manufacturer's instructions (Affymetrix). Synthesis of Biotin-labeled cRNA was performed using the Affymetrix GeneChip IVT labeling kit followed by purification with the sample cleanup module. Labeled cRNA was then fragmented and hybridized to Affymetrix GeneChip Human Genome U133Plus 2.0 arrays overnight. Hybridisation and scanning was performed in house at Barts Cancer Institute. For RT-qPCR analysis, RNA in the IP material was reverse-transcribed to cDNA using superscript III (Invitrogen) following the manufacturer's instructions. Quantitative real time PCR was performed on ABI7500 equipment using gene-specific primer pairs and amplification condition of 2 min at 50°C, 10 min at 95°C, and then 40 cycles of 15 secs at 95°C and 45 secs at 60°C.
Total RNA was isolated using silica-based spin-column extraction kit (RNeasy mini kit, Qiagen) following the manufacturer's protocol. Total RNA was treated with RNase-free DNase1 (Ambion) to reduce genomic DNA contamination. RNA integrity was evaluated using the Agilent Bioanalyzer. Two micrograms of total RNA was reverse transcribed with SuperScriptase III (Invitrogen) using Oligo-dT primers or random hexamers according to the manufacturer's protocol. Negative (-RT) controls contained RNase-free water substituted for reverse transcriptase.
Recombinant BORIS purification
The mammalian expression plasmid pM49-T4738 carries BORIS with an N-terminal HaloTag. Adherent HEK293T cells were transfected using Lipofectamine 2000 (Invitrogen) using standard methods. Cells were cultured for 48 h prior to harvest. Media were aspirated and cells washed in cold PBS before removal by cell scraping. Cells were centrifuged at 2000 ×
Western blot analysis
Protein extracts or precipitated protein complexes were separated on a 4–12% gradient NuPAGE polyacrylamide gel (Invitrogen) and then blotted onto nitrocelluose membrane (Invitrogen) as described by Jones et al.
In vitro binding assay
For RNA and DNA binding assays, ~1 mg of purified BORIS protein was incubated with 125 nM of each biotinylated homopolymer in 400 ml of Binding Buffer (RBB, 150 mM NaCl, 20 mM Tris (pH7.5), 1 mM dithiothreitol [DTT] and 0.2% NP-40 at 4°C overnight. Nucleotide:protein complexes were isolated by addition of 20 ml prewashed Dynabeads M280 Streptavidin (Invitrogen) to the reaction for 30 min rotating at room temperature. Complexes were magnetically captured and washed three times in RBB. After the final wash, beads were resuspended in 10 ml NuPAGE LDS sample buffer supplemented with 5 mM DTT, heated to 70°C for 5 min. Captured proteins were resolved by 4 - 12% SDS/PAGE and analysed by Western blot using anti-BORIS antibody.
Analysis of microarray data
Affymetrix Expression array files were analysed using Partek ® software, version 6.5 Copyright © 1993–2010 (Partek Inc.). Principle component analysis (PCA) was applied to identify any independent sources of variation in the data. We compared data for BORIS bound RNA transcripts with genome-wide gene expression profiles for each selected cell type (hNP1, 6dN and HEK293T cells) with at least two biological replicates. A t-test was performed and transcripts were considered to be preferentially associated with BORIS when the signals from the immunoprecipitated RNA fractions were enriched more than 2 fold, with a
Pathway analysis and functional classification
We used Protein ANalysis THrough Evolutionary Relationships (PANTHER) software to identify significantly enriched functional pathways and Gene Ontology (GO) terms associated with BORIS-bound transcripts
Quantitative real-time PCR
Both the published primers
Preparation and analysis of polysomes
Cell extracts for polysome analysis were prepared as described by Camacho-Vanegas O et al.
Cloning and transfection
The GFP-BORIS, GFP-CTCF and pEGFP-C3 vectors were transfected into HEK293T cells using FuGene 6-HD (Roche) according to manufacturer's protocol as previously described
Activation of relative TCF/LEF-dual luciferase assay
The effect of BORIS on the WNT pathway was evaluated by measuring the activation of transcription factor TCF/LEF with the Cignal TCF/LEF reporter assay kit (SA Biosciences). In the first instance, HEK293T cells were cells co-transfected with TCF/LEF reporter constructs and either C3-BORIS or C3-empty vector, using Lipofectamin-2000 (Invitrogen) according to manufacturer's instructions. In other experiments, non-targeted or β-catenin siRNAs (Ambion) were combined with the C3 BORIS or C3 empty vector and co-transfected with TCF/LEF reporter constructs (SA Biosciences) according to manufacturer's instructions. The TCF/LEF reporter used a mixture of an inducible β-catenin-responsive luciferase construct and a constitutively expressing Renilla element (40:1). After 48 hrs incubation cells were collected and analyzed for TCF/LEF activity using a dual-luciferase assay kit (Promega-Biosciences). TCF/LEF activation values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were done in duplicate, and the standard deviations are indicated.
6dN: hNP1 cells differentiated over 6 days; BORIS (CTCFL): Brother of the Regulator of Imprinted Sites; CTCF: CCCTC-binding factor; hNP1: Human neural progenitor cells; RPL7: Ribosomal protein L7.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
BWO and DS conceived the study and designed the experiments, BWO, TAJ, JA, JS, NO, DO, MAM, SP and CAP performed the experiments. TAJ performed and analysed Affymetrix array data, ARS provided reagents and advised on the manuscript, BWO, TAJ and DS wrote the paper. All authors read and approved the final manuscript.
This work was supported by Cancer Research UK programme grant C5321/A8318. HEK293T cells were a kind gift from Professor Aine MacKnight. The authors have declared that no conflict of interest exists.
CTCF: Master Weaver of the Genome
Tumor-associated zinc finger mutations in the CTCF transcription factor selectively alter tts DNA-binding specificity
Does CTCF mediate between nuclear organization and gene expression?
An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes
CTCF and its protein partners: divide and rule?
The evolution of epigenetic regulators CTCF and BORIS/CTCFL in amniotes
Molecular architecture of CTCFL
The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer
Reciprocal binding of CTCF and BORIS to the NY-ESO-1 promoter coincides with derepression of this cancer-testis gene in lung cancer cells
Loss of imprinting of IGF2 and H19 in osteosarcoma is accompanied by reciprocal methylation changes of a CTCF-binding site
Conditional expression of the CTCF-paralogous transcriptional factor BORIS in normal cells results in demethylation and derepression of MAGE-A1 and reactivation of other cancer-testis genes
Dynamic transcriptional regulatory complexes including BORIS, CTCF and Sp1 modulate NY-ESO-1 expression in lung cancer cells
CTCF regulates the local epigenetic state of ribosomal DNA repeats
Widespread Expression of BORIS/CTCFL in Normal and Cancer Cells
The potential of BORIS detected in the leukocytes of breast cancer patients as an early marker of tumorigenesis
BORIS, a paralogue of the transcription factor, CTCF, is aberrantly expressed in breast tumours
Selective apoptosis of breast cancer cells by siRNA targeting of BORIS
Epigenetic control of CTCFL/BORIS and OCT4 expression in urogenital malignancies
Expression of BORIS in melanoma: lack of association with MAGE-A1 activation
Genomic and expression profiling of human spermatocytic seminomas: primary spermatocyte as tumorigenic precursor and DMRT1 as candidate chromosome 9 gene
Global expression analysis of cancer/testis genes in uterine cancers reveals a high incidence of BORIS expression
DNA methylation-dependent regulation of BORIS/CTCFL expression in ovarian cancer
The cancer-testis antigen BORIS phenocopies the tumor suppressor CTCF in normal and neoplastic cells
BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma
Zinc finger proteins: getting a grip on RNA
The post-transcriptional roles of WT1, a multifunctional zinc-finger protein
Gene Expression Omnibus: NCBI gene expression and hybridization array data repository
CTCF and BORIS Regulate Rb2/p130 Gene Transcription: A Novel Mechanism and a New Paradigm for Understanding the Biology of Lung Cancer
BORIS/CTCFL-mediated transcriptional regulation of the hTERT telomerase gene in testicular and ovarian tumor cells
CTCFL/BORIS is a methylation-independent DNA-binding protein that preferentially binds to the paternal H19 differentially methylated region
The PANTHER database of protein families, subfamilies, functions and pathways
A molecular mechanism for the effect of lithium on development
mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum
The dissociation of rabbit reticulocyte ribosomes into subparticles active in protein synthesis
Isolation of a 5S RNA-protein complex from mammalian ribosomes
Having it both ways: transcription factors that bind DNA and RNA
Molecular basis for specific recognition of both RNA and DNA by a zinc finger protein
Multiple modes of RNA recognition by zinc finger proteins
Binding of Y-box proteins to RNA: involvement of different protein domains
Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins
Characterization of RNA aptamer binding by the Wilms' tumor suppressor protein WT1†
A cell cycle role for the epigenetic factor CTCF-L/BORIS
RNA binding by the Wilms tumor suppressor zinc finger proteins
Beyond DNA: zinc finger domains as RNA-binding modules
Dissecting the expression dynamics of RNA-binding proteins in posttranscriptional regulatory networks
Regulation of cerebral cortical size by control of cell cycle exit in neural precursors
Deciphering the function of canonical Wnt signals in development and disease: conditional loss- and gain-of-function mutations of beta-catenin in mice
Birchmeier C: beta-Catenin signals regulate cell growth and the balance between progenitor cell expansion and differentiation in the nervous system
Comparing protein abundance and mRNA expression levels on a genomic scale
Correlation of mRNA expression and protein abundance affected by multiple sequence features related to translational efficiency in Desulfovibrio vulgaris: a quantitative analysis
Long-term proliferation of human embryonic stem cell-derived neuroepithelial cells using defined adherent culture conditions
Cell cycle-dependent association of PML bodies with sites of active transcription in nuclei of mammalian cells
Eukaryotic mRNPs May represent posttranscriptional operons
GO::TermFinder—open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genes
BORIS (CTCFL) is not expressed in most human breast cell lines and high grade breast carcinomas
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
Growth-dependent and growth-independent translation of messengers for heterogeneous nuclear ribonucleoproteins


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|Dipl.-Ing. Dr. Johann Eder
Johann Eder is full professor for Information and Communication Systems in the Department of Informatics-Systems of the Alpen-Adria Universität Klagenfurt, Austria. From 2005-2013 he served as Vice President of the Austrian Science Funds (FWF). He held positions at the Universities of Linz, Hamburg and Vienna and was visiting scholar at AT&T Shannon Labs, NJ.
The research interests of Johann Eder are databases, information systems and data management for medical research. A particular focus of his work is the evolution of information systems and the modelling and management of temporal information and temporal constraints. Another focus is the application of Information technology for medical research from information systems for biobanking, information privacy to modelling of biological processes.
He successfully directed numerous competitively funded research projects on workflow management systems, temporal data warehousing, process modelling with temporal constraints, application interoperability and evolution, information systems modelling, information systems for medical research, etc.
Johann Eder has contributed to workflow systems and business process management for 2 decades, in particular in the area of workflow systems languages and architectures, exception handling, time management, and data management. His research led to the development of the commercial workflow management systems altavistaWorks and @enterprise.
Johann Eder authored one book, edited 22 books, proceedings, special issues of journals and published more than 160 papers in international journals and conference proceedings. See dbpl, google scholar and the research database of AAU for lists of publications.
He served in numerous program committees for international and acted as general chair / PC chair for CAiSE, ADBIS, BPM, CoopIS, and DAWAK conferences. He is editor and referee for international journals and conferences (including ACM TODS, ACM TOIS, IEEE TKDE, IEEE Transactions on Services Computing TSCSI, Information Systems, Artificial Intelligence Review, Dat and Knowledge Engineering, Computer Journal, JCSE, JUCS, DSS, JSS, etc.).
Johann Eder received the degrees of Diplom-Ingenieur and Doctor of technical sciences from the University Linz, Austria. From 1985 till 1990 he was assistant professor at the University Klagenfurt. In 1990 he was visiting professor at the University of Hamburg, Germany. From 1990-1991 he was associate professor (tenured) at the University of Vienna, Austria. From 1991-2005 he was full professor for information and communication systems at University Klagenfurt and he chaired the Department of Informatics-Systems from 1994- 2005, and 2009-2013. From 2005 to 2007 he was full professor for informatics (workflow) at the University of Vienna. In 1998 he was visiting scholar with AT&T Research Shannon Labs, NJ, USA.
Databases, Information Systems, Process Engineering, Interoperability, Research Methods in Computer Science
Research Topics:
Workflows and Process Aware Information Systems, Evolution of Databases and Information Systems, Temporal Aspects of Information Systems, Information Systems for Medical Research


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MJ and Landon are two blond college guys who had incredible chemistry from the moment they met at College Dudes 24/7. Maybe it's narcisicm that attracted these two blonds to each other or maybe they were both just plain horny, but you'd think the two of them were long-term lovers rather than having just been introduced to do a porn shoot. Landon is a 19 year old sophomore with a cock that's always hard and MJ is an 18 year old freshmen who's already proved himself as a bottom in several videos.
The way Landon takes over, you'd think he was an old hand at porn, but this is actually his first shoot with another guy. From the very start of the shoot, he pulls MJ to him with passion and enthusiasm.
Before you know it, it's blowjob time. You can see that MJ loves sucking on Landon's rock-hard pole, and Landon feeds his dick to the MJ with enthusiasm.
Landon also loves eating MJ's ass, and it's obvious he's done this before. He teases the bottom boy with his warm, wet tongue, stimulating that hot hole and getting it loose and ready!


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Investor Education
Charles H. Dow, Father of Value Investing
It's All About the Dividends
Dow Theory: Buying in Scales
How to Avoid Losses
When Dividends are Canceled
Cyclical and Secular Markets
Inflation Proof Myth
What is Fair Value?
Issues with P-E Ratios
Beware of Gold Dividends
Gold Standard Myth
Lagging Gold Stocks?
No Sophisticated Investors
Dollar down, Gold up?
Problems with Market Share
Aim for Annualized Returns
Anatomy of Bear Market Trade
Don't Use Stop Orders
How to Value Earnings
Low Yields, Big Gains
Set Limits, Gain More
Ex-Dividend Dates
Recent Posts
Quick Links
Inside a Moneymaking Machine Like No Other
The Fuzzy, Insane Math That's Creating So Many Billion-Dollar Tech Companies
Berkshire Hathaway Shareholder Letters
Forex Investors May Face $1 Billion Loss as Trade Site Vanishes
Why the oil price is falling
How a $600 Million Hedge Fund Disappeared
Hedge Fund Manager Who Remembers 1998 Rout Says Prepare for Pain
Swiss National Bank Starts Negative
Tice: Crash is Coming...Although
More on Edson Gould (PDF)
Schiller's CAPE ratio is wrong
Double-Digit Inflation in the 1970s (PDF)
401k Crisis
Quick Link Archive
"Guaranteed" Rates
Dow P/E: 21.36
Dow Yield: 2.33%
S&P 500 Yield: 1.97%
10-Year Note Yield: 2.50%
30-Year Note Yield: 3.11%
WSJ March 17, 2017
Dow Fair Value (50% Principle):
2015 High: 20,125.58
2009 Low: 6,469.95
Fair Value: 13,297.77
Current: 20,071.46
+33.75% above fair value
as of February 5, 2017
Downside Targets:
Index Additions and Deletions
Tools & Resources
Market News
- German Business Confidence Reaches New Record High – New York Times
- Arconic Weighs in on London Fire
- Michael Kors to Buy Jimmy Choo for $1.2 Billion
- Earnings Outlook: Chipotle earnings: Media coverage of the latest illness scare will put earnings targets at risk
- Here's what it looks like when the foreclosure 'pig' moves through the housing-crisis 'python'
- The Fed: Fed to stick to plans for rate hike, balance-sheet selloff this year
- Michael Kors Is Buying Shoemaker Jimmy Choo's For $1.2 Billion – Fortune
- Google Parent Posts Strong Ad Growth, but Earns Less for Each Click
- We Won't See You in Court: The Era of Tort Lawsuits is Waning
- SoftBank is reportedly keen to buy 'multi-billion dollar stake' in Uber – TechCrunch
- Expedia, Priceline Home In on Airbnb's Turf
- Can They Fire Me If I Already Gave Notice?
- Why Is My Manager Suddenly Hostile Toward Me? Here's Why
- This Is What Worries Me About 'Game Of Thrones' After Last Night's Episode
- Deregulation Could Lift Big Bank Profits 30%
- Flex Shares Could Rise 25% in a Year
- A Mini-Berkshire Ready to Break Out
- One Bank CEO's Rx for Deregulation
- The power of populists
- KKR, a private-equity giant, lays out its succession plan
- Reform of China's ailing state-owned firms is emboldening them
- Could bond funds break the market?
- Regulating credit unions in Africa


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improveand protect your home
Contact your local friendly builders
Our Services
Our friendly experienced team
Our Building Services
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We can provide free quotes on all prospective work and our prices are highly competitive.
pick up the phone now and call for your free quotation
0844 740 1895
Loft Conversions
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Painting and Decorating
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Tiling and Flooring
Brickwork and Pointing
Patios and Drives
Fencing and Decking
Windows and Doors
Guttering, Fascias and Soffits
Copyright ©2011 Arc Building. All rights reserved.


text:
Windows Phone's Cortana near shipped: Microsoft's Siri onboard
If what's said today about Microsoft turns out to be true tomorrow, Windows Phone users may be following the bouncing ball sooner than later. Foretold in 2013 by a lost Windows Phone, a personal assistant by the name of Cortana may be hitting the Microsoft software ecosystem very, very soon. This assistant has been suggested by The Verge to be hitting Microsoft devices "in the coming months."


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- Company Name:Construction Company
- Employment Type:Full Time
- Monthly Salary:Unspecified
- Benefits:as per uae law
- Minimum Work Experience:0-1 Years
- Minimum Education Level:High-School / Secondary
- Listed By:Employer
- Company Size:51-200 Employees
- Career Level:Junior
Required Warehouse Assistant/In- charge for Company. Asian nationality. Can supervise the junior staff. Can speak English. Fresher can apply also. For interview kindly contact on: 054-3537346


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World Tour Mixed 12-Pack
Your World Tour Mixed 12 Pack includes wines from California, Italy, and Chile, hand selected by our expert wine panel.
2013 Val di Botte Toscano Rosso – aromas of red cherries and raspberries, lead to lush flavors bursting with red fruits, sweet vanilla and orange notes.
2013 Big Cirrus Cabernet Carménère – This Chilean classic offers aromas of ripe raspberries and currants leading to representative fruit flavors with hints of herbs, subtle spices and vanilla.
2012 Cameron Hughes Lot 456 Lodi Red Blend – Aromatic and lavish with spicy raspberry, blackberry, juicy plum and floral notes lingering with a long and lasting finish. This beauty from Lodi, California is the perfect BBQ wine this summer.
2013 Qapay Reserve Cabernet Sauvignon – Rich Chilean wine, from the Maule Valley in Central Chile, offering intense dark berry flavors with an herbaceous vibe.
2013 Qapay Reserve Syrah – Flavors of blueberry, blackberry, and boysenberry accents of allspice, fresh herbs, and white pepper come together in a harmonious balance, in yet another Maule Valley gem.
2014 Cachil Reserve Cabernet Sauvignon – This reserve offering from one of the premier Cabernet producing regions of Valle Central, Chile boasts beautiful flavors of currants, raspberry, and cassis with hints of cocoa.
2014 Val di Botte Toscano Blanco – Pale, straw like in color, with pleasant, fruity aromas and hints of fresh flowers leading tointense fruits flavors like green apples, melon, and lemon zest.
2014 Val di Botte Pinot Grigio – This lowalcohol, balanced, acid-driven Italian Pinot Grigio flaunts citrus zest, apple, pears, and nectarine flavors mingling with slight almond and ginger notes, making it a quintessential summertime wine.
2013 Cachil Reserve Sauvignon Blanc – Bursting with citric fruit notes and subtle sweet aromas, this wine has a wonderful, crisp acidity and pronounced citrus flavors.
2013 Yapa Chardonnay – Golden yellow with a lovely greenish hue, refreshing aromas of ripe pineapple and white peach, and totally un-oaked style.
2013 Cameron Hughes Lot 479 Triage White Blend – This Riesling blend from Washington State is crisp, slightly sweet, with flavors of apricot, banana, and lemon custard. It finishes drier with a balanced juicy texture.
2014 Qapay Sauvignon Blanc – Clean, citrusy, lean and fresh wine with aromas of grapefruit and green apple followed by zesty flavors of green fruits, lime, floral notes and a crisp, refreshing finish.


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Houston Cellar Classic 2014 - The Grand Tasting
The Tasting Room - Uptown Park
Saturday, October 18, 2014 from 1:00 PM to 5:00 PM (CDT)
Who's Going
San Francisco, California
London, United Kingdom
Florence, Italy
Event Details
11th Annual Houston Cellar Classic GRAND TASTING
Sat., October 18, 2014 | 1 – 5pm | The Tasting Room-Uptown Park
Benefiting: St. Jude Children's Research Hospital
The 11th vintage of the Houston Cellar Classic signature event is quickly approaching! The Houston Cellar Classic is a celebration of the best food and wine that Houston has to offer. VIP and General Admission tickets are available for purchase. We hope you will join us to for the Grand Tasting Experience to combine great wine, delicious food, live music and a little friendly competition among the participating restaurants. If you have any questions, please feel free to contact us.
includes a tasting of up to 60 General Admission wines, PLUS 40 additional VIP wines + bubbles, access to all the food from participating restaurants + gourmet VIP-only food pairings, live music and high-end crystal stemware (to take home!)
includes a tasting of up to 60 wines, food from participating restaurants, live music, and crystal stemware (to take home!)
*Sales tax is added to ticket price at time of purchase.
Where can I contact the organizer with any questions?
Is my registration/ticket transferrable?
No. Tickets are only valid for that specific event but may be given to a family/friend if you cannot attend.
Do I have to bring my printed ticket to the event?
Yes, or you can show your ticket on your phone.
What happens if it rains?
Event will still go on. This is a rain or shine event.
What is the refund policy?
Non-refundable, but may be used by a family/friend if you cannot attend.
The name on the registration/ticket doesn't match the attendee. Is that okay?
Yes. But we will ask for the attendees contact information should the original ticketholder dispute the redemption.
When & Where
The Tasting Room - Uptown Park
1101 Uptown Park Blvd
Saturday, October 18, 2014 from 1:00 PM to 5:00 PM (CDT)
Add to my calendar


text: A lot of martial arts are considered sports when they are competitive. I did Tang Soo Do for a couple of years when I was a teenager and Aikido for 4 years in my late twenties. Its been a while but I am thinking of getting back into it. Does anyone have any recommendations? What martial arts have you had experience with and what was the experience like? There's an MMA group not far from where I live. I might go along and try it. Or I quite fancy trying kickboxing or muay thai.


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BikeHUD Adventure augments your motorcycle rides
Drivers have no lack of options when it comes to head-up displays (HUDs), but those who use a motorcycle to get around are less fortunate. Though we've seen some options for motorcyclists, not many are presently available on the market, and so any addition is a welcomed one. The BikeHUD Adventure is one of the newest models to surface, and as you'd expect it works in the same way as HUDs for cars, displaying things like speed and, in this case, gear so that users don't have to look down at the speedometer.


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TRANSITIONS® Angled Entry Mat
The TRANSITIONS® Angled Entry Mat – is accommodating for doorways inside and outside. It does not interfere with the door track and works especially well with sliding glass doors. It is made with 100% recycled rubber and provides a smooth and safe entry or exit. The Angled Entry Mat is available in three colors, Black, Storm Grey and Hazelnut Brown. Learn more about the TRANSITIONS Angled Entry Mat by exploring the tabs on this page!
This Product Resides in the Following CategoriesCommercialIndustrialResidential
Product Photos
Additional Photos
Product Specifications
The slip-resistant surface of the TRANSITIONS Angled Entry Mat is made with 100% recycled rubber and provides a safe transition both indoors and outdoors. It does not interfere with door tracks and is especially convenient for sliding glass doors. It is 36" wide and is available in a 1.5 and 2.5 inch heights.SPECIFICATIONSTAEM 1.5TAEM 2.5Made of 100% Recycled Rubber√√Slip-resistant Surface√√Usable Size (in) (H x L x W)1.5 x 14 x 362.5 x 25x 36Overall Size (in) (H x L x W)1.5 x 14 x 402.5 x 25 x 43Weight (lbs)*1431Weight Capacity (lbs)850850For Wheelchairs and Scooters√√Usage Guidelines**1.5" rise2.5" rise
* Weights are approximate and may vary.
** Consult your equipment's owner guide for proper degree of incline. Never exceed its recommendations.
For additional specs and dimensions see "Documents"
Product Features
100% Recycled RubberMade with 100% recycled rubber, the TRANSITIONS Angled Entry Mat is eco-friendly and can be used safely on nearly any surface. By nature, the recycled rubber material is slip-resistant, so users can feel secure and confident when using the TRANSITIONS Angled Entry Mat. It truly is a safe "welcome mat" for any home.
Beveled EdgesThe edges on the TRANSITIONS Angled Entry Mat have been beveled allowing cross-traffic access.
Indoor and Outdoor UseFor indoor or outdoor use, the TRANSITIONS Angled Entry Mat is accommodating for any user, anywhere.
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Seapine Software
Seapine Software, Inc. designs, develops, and markets software application lifecycle management solutions to the information technology sector. The company offers application testing, software change management, bug reporting, configuration management, and development process management services. Additionally, it offers data conversion, workflow management, script testing, and product training services.
With over 8,500 customers worldwide, Seapine Software, Inc. is a recognized, award-winning leading provider of quality-centric application lifecycle management (ALM) solutions. Seapine is uniquely positioned to directly provide sales, support, and services around the world with headquarters in Cincinnati, Ohio and offices in London, Melbourne, and Munich.
Seapine Software's integrated software development and testing tools streamline customers' development and QA processes, improving quality and saving significant time and money. Designed for the most demanding software development environments, Seapine's ALM solutions are scalable, feature-rich, team-based tools that can be used separately for superior issue tracking, software configuration management, automated software testing, or test case management—or seamlessly integrated for more efficient control of the application development process.
Seapine Software designs products focusing on various development environments and platforms that include Microsoft Windows, Linux, Sun Solaris, and Apple Macintosh. The company markets its products under different brand names such as TestTrack Pro-Development, workflow and issue management; TestTrack TCM-Test, case planning and tracking; Surround SCM-Software, configuration management; QA Wizard Pro-Automated, functional and regression testing; Seapine CM-Complete, software change and issue management; and TestTrack Studio-Complete, management of development processes, issues, and quality. It partners with ChangeGroup, Erudis, Head-On Computer Systems Ltd., Ideo Technologies, LogicaCMG, SageRight, Apple developer, and VMware.
Seapine Software was founded in 1995 and is headquartered in Mason, Ohio with additional offices in Munich, Germany; Vienna, Austria; Point Cook, Australia; and London, United Kingdom.
Seapine Defect Tracking Software Key Strengths
- Seapine Software is the leading provider of quality-centric application lifecycle management (ALM) solutions for product development and IT organizations.
- The company's mission is to equip its customers with the tools, technologies, and best practices to deliver quality software products on time and within budget.
- As a multiple product company with over 8,500 customers worldwide, Seapine Software is focused exclusively on the needs of software development organizations. The company is uniquely positioned to deliver innovative, tightly-integrated ALM solutions that improve product quality.
- Seapine Software is fiercely focused on winning hearts, minds, and markets by continuously exceeding customer expectations. Its passion for exceeding expectations extends to crafting the highest quality products, presenting the most professional public image, and creating a work culture of employee empowerment and career satisfaction.
Seapine's Integrated ALM Suite
- TestTrack Pro – Development workflow and issue management
- TestTrack TCM – Test case planning and tracking
- Surround SCM – Software configuration management
- QA Wizard Pro – Automated functional and regression testing
Seapine ALM Software Key Strengths
- Seapine Software's flexible cross-platform solutions adapt to the way teams work.
- Collaboration features allow organizations to achieve maximum productivity whether their teams are located under one roof or scattered around the world.
- Automation helps organizations deliver products faster by eliminating steps, streamlining processes, and automating repetitive tasks.
- Seapine application lifecycle management (ALM) tools provide a lower cost of entry. Unlike most ALM suites that require a dedicated IT staff to support them, Seapine's solutions can easily be managed by business professionals or the development staff, greatly reducing software management and support costs.


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Cascading crystal beads create a luxurious look and feel in this gorgeous 4-light chandelier. Chrome finish details add cool contemporary appeal. A white sheer drum shade offers a glimpse of the crystal beads for a beautiful effect to complete the style. From Vienna Full Spectrum.
Look to Vienna Full Spectrum to deliver innovative, European inspired designs that complement today's home décor. From chandeliers to sconces to pendants and even clocks, Vienna Full Spectrum has its finger on the pulse of the new millennium. We welcome you to discover the perfect complement for your home's décor by browsing our collection of beautiful Vienna Full Spectrum lighting fixtures.
Our team has over 100 years combined experience with contemporary lighting.
Every EuroStyleLighting.com order is backed by our 100% Satisfaction Pledge.
Shipping and Delivery Information - For item EU1F041 if ordered by 1 pm Pacific Time


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Aston Martin V12 Zagato Video Preview
Zagato, it's a name synonymous with style, grace and power. The double-bubble roof screams sex appeal and spy-like mystery that captures imaginations faster than flipping a light switch.
That's why we were so excited last week when images leaked of the new Aston Martin V12 Zagato, scheduled to make its public debut at the Kuwait Concours d'Elegance. Well, who likes waiting anyway? The good news is, there's a new video available today that showcases the V12 Zagato running down a road, engine roaring.
The 150-unit-limited beast is powered by a 6.0-liter V12 engine capable of 510-hp and 420 lb-ft of torque, pushing from 0-60 mph in 4.2 seconds.
Of course this, perhaps more than many other exotics, is reserved for the wealthiest connoisseurs with a roughly $520,000 price tag.
It's true that there are plenty of cars out there capable of more power, higher speeds and crazier shenanigans for a lot less coin, but with far less style, monsieur.
Watch the video and drink in the exhaust note after the jump.
GALLERY: Aston Martin V12 Zagato
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MONTVILLE TWP. - John Kraft of Waterloo Village will speak on the New Jersey Lenape Indians at the Montville Historical Society meeting at 7:30 p.m. on Monday, Jan. 14 in the Pio Costa Auditorium, Montville Library, 90 Horseneck Road.
Kraft will discuss the Indian way of life in New Jersey from prehistoric times to the settling of the Americas by other nationalities. He will discuss the Native American ways of hunting, fishing, and gatheringand the tools employed in these pursuits.
Large Lenape settlements were located at the border of Montville and Lincoln Park at Tom's Point, on Jacksonville Road and Pine Brook. This presentation was funded by the NJ Council for the Humanities. Refreshments will be served.


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Merry Christmas and Happy New Year to you and your family!
just dropping by my friends places to say hi
Thanks for the friend add! I am going to go add some love to your gallery. Have a wonderful week.
hello...i was just in your typepad site...very beautiful photos..would love to take pics like that !!! have a great week !!!
Merry Christmas and a Happy New Year "2009"
You and your family have a very Merry Christmas and a Happy New Year!
just stopping by to say hi! :waving: :-)
just dropping by to say hi lol alli xxx
Popping in to say hello! Hope you are having a fabulous weekend!!
You've been TAGGED, visit my blog.
Have a great Memorial Day Weekend!! :) --Shel
Hope you had a wonderful Mother's Day!
Would you like read a interview of "our founder" Jill Davis? read my blog!!!
LOL that you picked up on the BH sketch! I'd love to see yours ;)
Do you want to read an interview with one of the most Scrapbooking important gurus?
Here is an interview with lain Ehmann (Simple Scrapbooks magazine and Scraphappy blog)
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Happy Wednesday....it's all down hill from here!
Happy Valentine's Day!!
Happy Valentines Day!
*Amy* *Amy* *Amy* This is me chanting and cheering you on as you scrap today. GO girl!
Happy New Year. Hughs Elzie


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"Cops wanna knock me, D.A. wanna box me in. But somehow, I beat them charges like Rocky." - Jay-Z
Debt Free
When I graduated law school just a few years ago, there was a lot of talk about young lawyers declaring for bankruptcy to have their law school debts erased. Then they could waltz into some big firm making many Gs and not have the debt to repay. I'm not sure if anyone actually did it, but I know that some employers and some state bars were doing credit checks.
It's an interesting idea though. If businesses can use bankruptcy to "restructure," why can't enterprising young attorneys? Not that I know much about bankruptcy, of course.
9 comments:
I've been told that federal loans do not get discharged through bankruptcy (although I don't know if that's the same for all educational loans, or just the government ones). Which is a total bummer.ReplyDelete
Well, I do know one thing. Federal student loans are cancelled upon death.ReplyDelete
That has to be a starting point to a good scheme somehow.
OK maybe this dates me.ReplyDelete
My state used to really believe in higher education. We also used to have a lot of oil money. I paid a whopping $4.00 per semester hour to attend all of law school.
My average bill for tuition/fees/books was about $700-$1000 per semester when I attended law school.
Talk about a state welfare system.
- AnonymousDecember 22, 2004 7:44 AM
Federal loans arent' discharged through banrupcy. But the guy got a big firm job, a lot of credit from the credit card companies, used their "convenience checks" to pay off the loans, and then declared bankrupcy. Viola. Student loan debt gone.ReplyDelete
That was a problem when I was getting admitted into the bar and was asked repeatedly about this issue during the interview.
"Why can't enterprising young attorneys?"ReplyDelete
Because it would be a sneaky, lying, unethical way to get out of a debt. The school loan was a promise to pay the government back later, when the law student could better afford it. Based on that promise, the government advanced educational funds.
Any lawyer who tries to get out of paying back such a loan is a liar and a cheat, and should be disbarred. Then prosecuted.
- AnonymousDecember 22, 2004 10:55 PM
But the enterprising young attorney DID pay his loan back, but through the use of credit cards. The government is not being cheated at all, they got their dough. It's the credit card companies, the ones that charge you fees for doing anything, that are getting cheated presumably. It's the same thing as any major airline or any company filing for banrupcy and getting out of it.ReplyDelete
- AnonymousAugust 08, 2005 4:08 AM
I agree. All school loans should be payed. It is the persons responsibility to the govt. Otherwise, he won't get the education he/she got.ReplyDelete
- AnonymousAugust 08, 2005 4:09 AM
I agree. All school loans should be payed. It is the persons responsibility to the govt. Otherwise, he won't get the education he/she got.ReplyDelete
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The One Truth kata was based on Kakita's principle that a true master of the blade needed only one strike to end any conflict - sometimes less than that. Though not as esoteric as the bushi of the Shiba or Mirumoto families, this style focused students' chi in a way that could only be described as supernatural, forcing opponents into ineffective action or complete inaction. The One Truth kata was a graceful exercise that focused the bushi's mind and spirit in such a way that others were awed by his presence. [1]
- ↑ Legend of the Five Rings; Third Edition, p. 197
|This Crane Clan related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


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Unfortunately, If I re-visited former Rheumatologist's and Doctors and started doing somersaults and back flips and asked for their testimonial, they would just think I had taken some super powerful drug I obtained from somewhere, or that I've had double hip and knee replacement, or still taking mediation!
Why would the Doctor's believe me or even care!? - me telling them "I'm OK now", would be the equivalent of me telling my "milkman" I have recently bought some cows. He's not going to be interested in me anymore, and why should he.
The amount of people who keeps saying "Your looking well". "You look good". Others who know me better said I am "walking normally now", and that I don't look like I'm in pain.
Another acquaintance said "you're looking amazing, you're not like".. He then proceeded to hunch his body like I used to be.
My neighbor told me I'm "looking and walking much better" although he still thinks I'm taking Tramadol painkillers.
I explained what I had been doing to another neighbor I have not seen for months who said "It looks like there's nothing wrong with you now, your a miracle, I am so impressed". Well, it would have been a miracle if I had done nothing.
Press / Documentary.If I liked the lime light and attention, I would have contacted the media and revealed my story for others to share. Then the press could arrange for me to be tested to see that I am not on any medication, nor has my body had for at least nine months. They could then X-Ray and scan all my joints again to see the improvements. Then take me back to all the doctors, Rheumatologists and physiotherapist I saw and ask for their description of how I was previously to now.
The Global Positioning Satellite, (GPS).
My smart phone can detect I am walking faster and further as the months go by.
The only reason I would like to contact the press is 1, so my story can be made public for as many people possible to read, and 2. If my story 'was' published, and not just here in my wee blog, then perhaps people would not just believe, but to give them hope that it really is possible to reverse a crippling, painful, progressive incurable disease and go for it.
Read my post about other people who also decided to ditch their pills, exercise and eat healthily instead. Plus, it reveals the truth about Doctors and the Pharmaceutical companies.
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Lisa, lucky mama of Kelly (3/93) , Emma (5/03) , Evan (7/05) , & Jenna (6/09)
Loving my amazing dh, James & forever missing Aaron Ambrose (11/07)
My mom alway stuffed it and we never had any issues with food poisoning. And I have to say, she is very lax about that kind of stuff.
I actually don't like the stuffing from inside the turkey, and since Thanksgiving is at my house and I cook the turkey, I realllly don't want to stuff it. But last year when I mentioned it my sister had a freak out - apparently she just really loves the stuffing from inside the turkey. We bake stuffing on the side and it is never dry - I mix it with eggs and milk and baste it with turkey drippings along the way. Yummy!
But Alton Brown (among others) bring up the point that a stuffed turkey has to be cooked longer, which can dry out the turkey meat.
|I'm torn about what to do. I don't want to stuff it, but I don't want to deal with a hissy fit by my sister on Thanksgiving about how we don't care about her or her preferences, blah blah blah.
Oh, the stuffing recipe I use is an Apple Onion Stuffin' (Muffin) recipe from Rachael Ray. I may not be fond of her, but the recipe is great. And I'm lazy and just throw it all into a 13x9x2" baking dish rather than muffins, turns out great - even reheated it's not dry that I remember, and if it did, just sprinkle on some chicken stock until rehydrated again - easy peasy. Goes well with the fresh cider and apple pie and whatnot, too.
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Chevrolet car, truck, or SUV enthusiasts have become used to a certain level of reliability when driving their C2500 around town. Make no mistake, you bought your C2500 because you wanted to buy performance and American style both impeccably combined into one vehicle. The vehicle in your driveway is head and shoulders above the rest compared to all the other cars, trucks, and SUVs on the road today. You purchased your C2500 because its reliability and value appealed to your senses; so why would you be okay with second rate when it comes to superior quality auto parts?
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Product Description
Double Gold Medal Winner - San Francisco World Spirits Competition
"An undeniably modern take on gin, reflecting the tastes and preferences of the younger generation. This floral and fruit-forward gin is bottled at 47.6 percent alcohol by volume. and expresses the essence of three coveted ingredients: Turkish rose, white peach and raspberry."


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PAUL FRANK - Sock Monkey
someone to ride shotgun so you can drive in the HOV lane while trying to get through vancouver to the island... ha ha
Remember how much fun it was to ride a hobby horse as a toddler? It's just as much fun to watch your toddler ride a hobby horse... or rabbit?Added 7 Years Ago from Nest-living
I'm assuming this is a Paul frank product since the interior is covered with his little monkey character. I love rockers. The motion is so comforting. Great toy for kids.
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Oh the conversations you will get to overhear when your kid wears this hoodie! Sizes 2T-6X.
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A scooter with a Paul Frank motif ! Your child may not realize how cool they sitting atop this Vespa -for- toddlers, but you will. And since it maneuvers so well you won't mind letting the Uroll inside on a rainy day.Tags: toys, scooters, Paul FrankAdded 10 Years Ago from Modernseed


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Discussion in 'Off Topic' started by majdi, Aug 28, 2010.
I have tested many and i like iOS for its simplicity and speed.
iOS. I love my iPhone 3GS.
Android fan all the way! Right now rocking a pretty slow Motorola Cliq, but hopefully the G2 will be replacing it within the next month or so. But....
I like the iOS in terms of design, layout, and ease-of-use. Yes, I'm a huge Android Fandroid, but I can't excuse the fact that the interface could use a major overhaul. Here's to hoping that Gingerbread brings us that much-needed change!
iPhone <3
I have an iphone 3 and an Android (EVO).
I love my EVO!
Apple operating systems have my +1
-1 for proprietary locked down operating systems
I still really like my Palm Pre. Android might be interesting as well if HP doesn't get things done with WebOS.
Android, no doubt. Even though I do enjoy iOS too.
I use an iPhone 3Gs
IOS, iPhone 3GS
Maemo / MeeGo !
Blackberry 8700C.It out of date.
I've had the iPhone since it came out. My iPhone 4 is my second iPhone.
Im a Crackberry.
can an android fan tell us whats good about it?
i had a samsung galaxy s for a week and i didn't like it because it felt a little too complicated and geeky, the market app isn't near apple's and it isn't as fluid as apple's iphone
If I ever make the drunk and bad mistake about buying a blackberry, I want it to be blue, so I can call it my blueberry
Because it's geeky.
Google admits the UI isn't as smooth or intuitive as it should be, but version 3.0 (Gingerbread) will have a complete UI overhaul, so hopefully it'll be more like Apple's in terms of ease-of-use.
Ever played with HTC sense UI??? It is very very smooth and intuitive ... saying that however I am looking forward to Gingerbread.
android for me is about familiarity, coding for android is just as simple as coding for a desktop OS, expecially with things like flash support and AIR2 support (currently private beta) its only going to get easier...
android is more open, this is bad sometimes with the 70million pointless apps in the market, but it also means that apps that would never make it to the iPhone or would take months of waiting for approval are on android... Hell I went to the US and made all my calls using SIP over wifi... instead of costing me £1.69 a min, it cost be £0.01... Win
Blackberry but planning to switch to iPhone 4 when my current contract expires on December
Separate names with a comma.


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Daily Devotional
Pray About Everything
by Joyce Meyer
The earnest (heartfelt, continued) prayer of a righteous man makes tremendous power available [dynamic in its working].
—James 5:16
Driving down the road one day, pondering an upcoming change in my life, I found that I was afraid. It really wasn't a major thing, but it felt like it to me.
God spoke to me that day and simply said, "Pray about everything. Fear nothing." He showed me He couldn't work through my fear, but if I would give Him my faith, He would help me in my situation. I needed it that day for something seemingly minor, but I have used it many times since for all types of situations.
Isn't it good to know that God cares about everything that concerns you—even the little things you're afraid of? Your part is to pray and have faith, and God's part is to provide the power to meet your need. What do you need to pray about tonight?
From the book Ending Your Day Right by Joyce Meyer. Copyright © 2004 by Joyce Meyer. Published by FaithWords. All rights reserved.


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- also available in a coloured version (see the Helene model)
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Your made-to-order radiator
Each radiator is manufactured individually for you!


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Nursing assistants play an important role as part of the healthcare team by providing direct patient care and emotional and physical support for patients. The majority of nursing assistants are Certified Nursing Assistants (CNAs), who have completed training in basic patient care. CNAs are employed in hospitals, nursing homes, medical offices and other health care settings.
Job opportunities in the field of nursing assisting are expected to grow at a much faster than average rate over the next decade, according to the Bureau of Labor Statistics. Both CNAs and non-certified assistants can work in a wide variety of health care settings, as well as in the area of home health care. Average earnings for nurses aides as of 2006 (per the BLS's Occupational Outlook Handbook) was $10.67, with salaries for home health aides slightly lower and those of psychiatric aides slightly higher. Full-time positions in the field generally offer health insurance and other standard benefits.
Certified Nurses Aide training courses are offered through community colleges and agencies such as the Red Cross, and last anywhere from one to six months. CNA classes are also offered through some health care facilities, in exchange for an agreement to work at the facility for a specified period of time upon graduation. Coursework for CNAs is primarily hands-on and includes training in basic patient care procedures, maintaining hygiene and sanitation, proper handling of biohazardous materials, patient safety and medical terminology. In order to be eligible for licensure as a CNA, students must successfully pass a professional board examination after completion of the training program.
CNAs provide much of the direct care that is given to patients, particularly in home health care or nursing home settings. Nursing assistants help patients with personal care tasks such as feeding, toileting and bathing, as well as transporting patients and providing support to both the patient and his or her family. They also monitor the patient's condition, taking vital signs and maintaining or checking medical and personal equipment such as catheters. CNAs assist patients with wheelchairs or other assistive devices and may lead patients in therapeutic exercise or other activities to increase strength and range of motion.
Aside from direct patient care duties, CNAs are responsible for general care of the patient, such as ensuring that his or her room is clean. In most facilities, it is the nursing assistants who change linens and assure that the patient's immediate area is sanitary. Another very important duty of the CNA is to document all care that they give as a part of the medical record. For example, CNAs record vital signs or urine input/output levels.
Nursing assistants enjoy the benefit of knowing that they are helping people every day. However, they also work under conditions that can be both emotionally and physically demanding. Nursing assistants must have patience and maturity to be able to deal with patients who are abusive or upset, as well as working with individuals when they are frightened. It is also important that nursing assistants are comfortable taking direction from other team members and have excellent communication skills, both written and verbal.


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If you are looking for pop up booths that do not only have an eye-catching design, but is also lightweight and easy to set up, look no further than the 8 Foot Curved Graphic Table Top Displays. The frame simply pops open and locks into place, making set-up a breeze. Our inkjet booth graphics are clean and crisp, complementing your tabletop booth. For a little extra sparkle to your display, add some of our accessories, including lights, a literature rack, or shelves.
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Give us a call today at 1.877.215.5355 to talk about show displays, exhibits, flooring, and booths. Our knowledgeable customer service staff can help you to plan your next show. You can also send an email to our Sales staff through our "Contact Page," and they will gladly answer your questions about trade shows.
Customer Support If you have any questions, please give us a call! We answer our phones, and can provide quick and easy answers for any questions or concerns you might have.
We Serve the U.S. and Ship Worldwide We serve all U.S. cities and every convention center in the United States. We have customers in Seattle, Canada, Puerto Rico, Japan, and throughout the world. We can ship to the location of your event or anywhere you need your products delivered.