text:
|Publication number
|Publication type
|Application number
|Publication date
|Oct 29, 2002
|Filing date
|Apr 11, 2000
|Priority date
|Apr 11, 2000
|Fee status
|Also published as
|Publication number
|Svetlana Velkovska, Kerwin Ng, Haim Kedar
|Original Assignee
|Affymax, Inc.
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (56), Non-Patent Citations (19), Referenced by (16), Classifications (25), Legal Events (6)
|External Links: USPTO, USPTO Assignment, Espacenet
This invention relates generally to the handling and processing of solid supports, such as beads. More specifically, the invention relates to systems and techniques for placing solid supports into an organized array.
In modem chemistry, large libraries of chemical compounds are often created from which certain compounds which are of interest may be identified. The creation of such libraries may be accomplished with a synthesizing process where various compounds are placed on solid supports, such as spherical beads. Such processes are well known within the art, and are described in, for example, U.S. Pat. No. 5,503,805, the complete disclosure of which is herein incorporated by reference.
After synthesis, it is often desirable to analyze the compounds synthesized on the beads. One such process is by placing an assortment of beads in an assay solution and observing whether a chemical reaction occurs. For example, groups of beads which include synthesized chemicals may initially be placed into a plurality of wells where a portion of the compound on the beads is photo-chemically released. Assays are then performed on each well. For wells producing a positive result, the groups of beads are then transferred into another well or are further divided and placed into a plurality of wells so that further testing can occur to identify the compound. Hence, to successfully perform such assays, the beads need to be transferred to different locations.
When handling such beads, the beads are usually held within a liquid medium, such as water, to help prevent (among other reasons) the beads from sticking together. Usually, such beads are very small, e.g. on the order of about 5 μm to 300 μm. As such, transferring of such small beads from one location to another while held within a liquid medium may be difficult and challenging. Transferring such small beads is especially challenging when a known quantity of beads, such as a single bead, must be individually transferred from a group of beads and placed in a test well. Further, it is often convenient to separate the beads into arrays that are compatible with automated screening systems. For example, much of the automated equipment now uses standard footprints, such as 96 well plates, 864 well plates, and the like.
Exemplary techniques for separating and organizing solid supports are described in, for example, U.S. Pat. No. 5, 722,470 and U.S. patent application Ser. No. 08/637,123, filed Apr. 24, 1996, the complete disclosures of which are herein incorporated by reference. The invention is related to other techniques for separating solid supports and placing the solid supports into an organized array. The techniques of the invention permit the transferring of a single bead, or a known quantity of beads, in a repeatable manner.
The invention provides for the transfer of solid supports from one location to another. One particular feature of the invention is the ability to transfer a known quantity of solid supports in a repeatable and efficient manner. In one embodiment, a system for processing a plurality of solid supports comprises a tubular member having a proximal end, a distal end, and a lumen terminating at the distal end. A stop is positioned with the lumen at a location spaced above the distal end and is sized to permit fluids to pass through the lumen while preventing the passage of solid supports. A fluid transfer device is provided to transfer fluids through the lumen such that when a fluid containing multiple solid supports is aspirated into the lumen, one or more solid supports may be drawn into the lumen until lodging against the stop. Hence, a known quantity of solid supports may be captured within the lumen simply by adjusting the position of the stop relative to the distal end of the tubular member. Once the solid supports are captured, the tubular member may be transferred to another location and the solid supports expelled by forcing liquids out of the distal end using the fluid transfer device.
In one particular aspect, the fluid transfer device comprises a syringe pump and a length of tubing coupling the syringe pump to the tubular member. In this way, the syringe pump may be operated to aspirate fluids into the tubular member to capture one or more solid supports into the lumen, and to irrigate fluids from the tubular member to expel any captured solid supports. Conveniently, a source of fluid may be coupled to the syringe pump.
In another particular aspect, the stop comprises a wire that is slidable within the lumen to permit the spacing between the distal end of the wire and the distal end of the tubular member to be adjusted. In this way, the number of potential solid supports that may be captured within a lumen may easily be adjusted.
In still another aspect, a reservoir is provided having a plurality of solid supports. A moving mechanism is also provided to move the tubular member relative to the reservoir to permit placement of the distal end of the tubular member into the reservoir. The system may also include a plate having a plurality of wells, and the moving mechanism may be configured to move the tubular member relative to the plate to permit any solid supports to be expelled from the tubular member and into the wells of the plate. In one particular aspect, the moving mechanism comprises a movable arm to move the tubular member along the Z axis, and a movable stage onto which the reservoir and the plate are held. The stage is movable along the X and Y axes so that the plate may be appropriately positioned relative to the tubular member. Conveniently, a controller may be provided to control operation of the fluid transfer device and the moving mechanism.
Advantageously, the system may include a plurality of tubular members that each have a lumen and a stop disposed within the lumen in a manner similar to that just described. A manifold may be coupled to each of the tubular members, with the fluid transfer device being coupled to the manifold. In this way, the throughput of the system may be greatly increased by introducing the tubular members into an array of reservoirs so that each tubular member may capture one or more solid supports.
In another particular aspect, the lumen may have a diameter in the range from about 190 μm to about 210 μm, and the solid supports may have a mean diameter in the range from about 120 μm to about 140 μm. Further, the wire may have an outer diameter in the range from about 125 μm to about 150 μm to permit fluids to pass between the wire and the tubular member when aspirating and irrigating fluids. However, it will be appreciated that other sizes may be used. For example, in some cases the lumen may have a diameter in the range from about 100 μm to about 250 μm.
The invention further provides an exemplary method for processing solid supports having at least one chemical that is synthesized onto the solid supports. According to the method, a tubular member is provided having a proximal end, a distal end, and a lumen terminating at the distal end. A stop is positioned within the lumen at a location spaced above the distal end. The distal end of the tubular member is placed into a fluid having a plurality of solid support, and at least some of the fluid is aspirated into the lumen. The aspirated fluid travels past the stop to draw at least one of the solid supports into the lumen until lodging against the stop. The liquid may be aspirated for a time sufficient to permit the space between the stop and the distal end to be filled with a linear array of solid supports. For example, the tubular member may be employed to capture about one to about 10 solid supports within the lumen, although in some cases larger amounts may be captured.
After the solid supports have been captured within the lumen, the tubular member may be removed from the liquid and transferred to another location. Fluid may then be forced through the lumen and past the stop to expel the solid supports from the tubular member. Conveniently, the fluid and the solid supports may be held within a reservoir, and the tubular member may be moved relative to the reservoir to place the distal end into the fluid. After removing the tubular member from the reservoir, the tubular member may be moved over a multi-well plate to permit the solid supports to be expelled into one of the wells of the plate.
In another aspect, the lumen may be primed with a fluid to remove substantially all gas bubbles from the lumen prior to aspirating the fluid. In another aspect, multiple tubular members may be provided that each have a lumen and a stop within the lumen. In this way, a distal end of each of the tubular members may be simultaneously introduced into the fluid, and fluid aspirated into each of the lumens to draw at least one solid support into each of the lumens.
FIG. 1 is a schematic diagram of a system for processing solid supports according to the invention.
FIG. 2 is a schematic. diagram of an alternative system for processing solid supports according to the invention.
FIG. 3 is a more detailed view of an array of tubular members employed to capture and transfer solid supports.
FIG. 4 is a front perspective view of one exemplary embodiment of a multichannel capillary manifold that may be employed to simultaneously process multiple solid supports according to the invention.
FIG. 5 is a front view of the manifold of FIG. 4.
FIG. 5A is a cross-sectional side view of the manifold of FIG. 5 taken along lines A—A.
FIG. 5B is a more detailed view of a tubular member of section B of the manifold of FIG. 5A.
FIG. 5C is a more detailed view of a wire of section C of FIG. 5A.
FIG. 6 is an exploded view of the capillary manifold of FIG. 5A.
FIG. 7 is a flow chart illustrating one method for processing solid supports according to the invention.
The invention provides techniques for transferring a known quantity of solid supports in a repeatable manner. The techniques may be used to transfer a single solid support or a group of known size. For example, the techniques may be employed to transfer a group that is the range from about 2 to about 10 solid supports in a repeatable manner so that each time a group is transferred, it will contain the same number of solid supports.
In one aspect, the solid supports processed by the invention may comprise small beads onto which chemical compounds have been synthesized. Such beads are typically small, usually being on the order of about 5 μm to about 300 μm in diameter, and more usually from about 80 μm to about 200 μm. The chemicals may be synthesized onto the solid supports using any one of a variety of techniques as is known in the art, including those described in U.S. Pat. No. 5,503,805, previously incorporated by reference. Such beads may conveniently be constructed of a polymer, such as a polystyrene and polyethylene glycol, and are generally sperical in geometry. Such beads are available from a variety of commercial suppliers, including, for example, Nova BioChem.
One use of the invention is to facilitate screening of the chemical compounds following synthesis. For example, following synthesis, the beads may be placed into one or more common storage locations or reservoirs and stored in a random manner. The invention may be utilized to systematically transfer one or more beads from the reservoir or reservoirs to permit them to be placed into the wells of a multiwell plate where the solid supports may be screened using common screening equipment and techniques. For example, once the beads have been transferred into the wells of the plate, at least some of the compounds may be released from the solid support so that assays may be performed on the released compounds. The assay results may then be measured and certain solid supports may be chosen for decoding in order to identify or further evaluate the compounds. To facilitate this process, the invention may be used to remove the beads from the wells of the multiwell plate for transfer to another location, such as to the wells of another multi-well plate.
The invention facilitates the processing of solid supports with the use of a tubular member having an internal lumen. A stop is placed within the lumen at a location that is spaced apart from the distal end of the tubular member. The stop is configured so that liquids may be aspirated or irrigated through the lumen around the stop. In this way, the tubular member may be placed into a reservoir containing a liquid and solid supports, and liquid aspirated into the lumen until one or more solid supports are drawn into the lumen and engage the stop. The tubular member may then be removed from the reservoir and transferred to another location where a liquid may be forced through the lumen to expel the solid support. Although the invention may utilize a single tubular member, in some cases it may be desirable to include an array or manifold of tubular members to permit the parallel transfer of multiple solid supports or multiple groups of solid supports.
Referring now to FIG. 1, a system 10 for processing solid supports will be described. System 10 comprises a capillary tube assembly 12 that is constructed of a capillary tube 14 having a proximal end 16, a distal end 18, and a central lumen 20 extending between proximal end 16 and distal end 18. Conveniently, tube 14 may be constructed of a polyimide tubing, commercially available from Cole-Parmer. Positioned within lumen 20, is a wire 22 having a distal end 24 that is spaced apart from distal end 18 of capillary tube 14. Such a spacing may be varied depending upon the number of beads 26 that are to be captured within lumen 20 as described hereinafter. Wire 22 has an outer diameter that is smaller than the inner diameter of capillary tube 14 to permit liquids to flow through lumen 20 when capturing or expelling the beads.
Coupled to proximal end 16 is a connector 28 to provide a fluid connection between capillary tube 14 and a syringe pump 30 via a line 32. Syringe pump 30 comprises a housing 34 that includes a syringe mechanism 36 and a valve structure 38. Valve structure 38 includes a connector 40 to facilitate coupling of line 32 to syringe pump 30. Valve structure 38 also includes a connector 42 to permit a liquid supply 44 to be coupled to syringe pump 30 via a line 46. Valve structure 38 is configured so that when syringe mechanism 36 is extended, liquid from supply 44 is drawn through line 46 and into syringe mechanism 36. When syringe mechanism 36 is compressed, the captured liquid is forced out of connector 40 and through line 32 where it flows through capillary tube 14. One example of such a syringe pump that is configured to operate in this manner is a XL 3000 Modular Digital syringe pump, commercially available from Cavro Scientific Instruments, Inc. However, it will be appreciated that other types of syringe pumps, as well as other types of fluid transfer systems may be employed to irrigate liquids through lumen 20 as well as to aspirate fluids into lumen 20.
System 10 further includes a reservoir 48 containing a supply of beads 26 which are held within a liquid 50. Beads 26 are generally spherical in geometry and may include synthesized chemicals that form a combinatorial library of chemical compounds as previously described. The types of liquid in which beads 26 may be stored include liquids such as water, 50% ethanol in water, and the like. Typically, the liquid within supply 44 will comprise the same type of liquid held in reservoir 48. System 10 also includes a plate 52 having a plurality of wells 54 for receiving one or more beads that have been transferred from reservoir 48. Conveniently, plate 52 may be constructed to have a standard footprint so that it may be useful with standardized equipment when screening the chemical compounds, as well as for other processes as is known in the art.
In one method of operation, beads are transferred from reservoir 48 to wells 54 by first operating syringe pump 30 to fill syringe mechanism 36 with liquid from supply 44. In this way, line 56 is completely filled with liquid. Syringe pump 30 is then operated to force liquid through line 32, thereby priming line 32 and filling lumen 20. Preferably, syringe pump 30 is operated until lumen 20 is substantially completely filled with liquid to remove any gas bubbles. Capillary tube 14 may then be inserted into reservoir 48 and syringe pump 30 may again be operated to irrigate a small amount of fluid through lumen 20 to ensure that no gas bubbles exist within lumen 20 as well as to compensate for the amount of liquid that will be lost when beads 26 are aspirated into capillary tube 14. In some cases, the released liquid may also serve to provide a small stirring effect on beads 26 to facilitate their capture into lumen 20.
Syringe pump 30 is then operated to aspirate liquids into lumen 20. As liquid from reservoir 48 is aspirated into lumen 20, some of beads 26 are drawn into lumen 20 as shown. The number of beads that may be captured into lumen 20 depends on the distance between distal end 24 of wire 22 and distal end 18 of capillary tube 14. As shown, three beads have been captured in a linear array. However, it will be appreciated that other numbers of beads may be captured during a single picking operation. For example, wire 22 may be configured so that only a single bead is captured during each picking step. Alternatively, groups from about two to about 10 beads may be captured at a time. Configuration of capillary tube assembly 12 is advantageous in that a known quantity of beads may be captured each time a picking operation is performed. In this way, a known quantity of beads may be captured and transferred in a repeatable and efficient manner.
Syringe pump 30 is operated for a time sufficient to allow the beads to be captured within lumen 20. After a certain time period, operation of syringe pump 30 is ceased and capillary tube 14 is removed from reservoir 48. Capillary tube assembly 12 is then moved over plate 52 until capillary tube 14 is aligned with a desired well 54. Syringe pump 30 is then operated to force liquid through line 32 to expel the captured beads into the aligned well. Capillary tube assembly 12 may then be moved over reservoir 48 and the process repeated to transfer beads 26 into the desired number of wells. Plate 52 is then ready for appropriate biological screening or other processing as is known in the art.
In some cases, it may be desirable to increase the number of capillary tubes in order to increase the number of beads that may be transferred during a single picking operation. One system 56 that includes such features is illustrated in FIG. 2. System 56 comprises a capillary tube manifold assembly 58 which includes an array of capillary tube assemblies that may be constructed in a manner similar to that previously described in connection with FIG. 1. Hence, for convenience of discussion, the capillary tube assemblies will be described using the same reference numerals used in describing capillary tube assembly 12 of FIG. 1. For convenience of illustration, capillary tube manifold assembly 58 is shown in greater detail in FIG. 3.
Fluidly connecting each capillary tube assembly 12 is a fluid manifold 60. As shown, assembly 58 includes eight capillary tubes 14. Further, eight fluid delivery lines 62 are coupled to manifold 60. Fluid lines 62 are in turn coupled to a syringe pump 64. However, it will appreciated that other numbers of capillary tubes may be incorporated into manifold assembly 58. Further, although a separate fluid delivery line is provided for each capillary tube 14, it will be appreciated that manifold 60 may be configured to evenly distribute fluids or provide suction to each capillary tube 14 so that only one fluid delivery line would be needed. In such a case, syringe pump 64 may be configured to be essentially identical to syringe pump 30 of FIG. 1.
Syringe pump 64 comprises a housing 66 that includes multiple syringe mechanisms 68 that may be similar to syringe mechanism 36 of FIG. 1. Valve structures 70 are also provided to permit coupling of syringe mechanism 68 to fluid delivery lines 62. Also coupled to valve structure 70 are fluid delivery lines 72 that terminate at a manifold 74 which receives liquid from a liquid supply 76 via a line 78.
With such a configuration, syringe pump 64 may be operated to fill syringe mechanism 68 with liquid from supply 76. Syringe pump 64 may then be operated to prime lines 62 and to aspirate liquids into capillary tubes 14 to capture beads within capillary tubes 14 in a manner similar to that previously described in connection with FIG. 1.
System 56 further includes a movable stage 80 which is movable along X and Y axes as indicated by the arrows. Positioned on stage 80 is a multi-well plate 82 having a plurality of wells 84 as is known in the art. Also held on stage 80 is a plate 86 having a plurality of reservoirs 88 that each include multiple solid supports. Conveniently, reservoirs 88 may include beads which originated from the same or from a different synthesis pool/library. Use of reservoirs 88 is advantageous in that the beads may be stored within plate 86 without experiencing cross-contamination. Although not shown, a mechanism is also provided to translate manifold assembly 58 along the Z axis to permit capillary tubes 12 to be moved into reservoirs 88 and into wells 84 as described hereinafter. Conveniently, a controller 90 is provided to control movement of stage 80, movement of the mechanism to move manifold assembly 50 and to control operation of syringe pump 64. In this way, the transfer of beads may occur in a fully automated manner.
For example, one method of operation for transferring beads from reservoirs 88 to wells 84 is by using controller 90 to operate syringe pump 64 to fill syringe mechanisms 68 with liquid from supply 76 as previously described. Controller 90 may then send a signal to syringe pump 64 to prime lines 62 and lumens 20. Controller 90 may then send a signal to stage 80 to align reservoirs 88 with capillary tubes 14 and to move manifold assembly 58 downward until capillary tubes 14 are within reservoirs 88.
Once capillary tubes 14 are within reservoirs 88, controller 90 may be operated to further prime the lines and to expel any liquid in order to compensate for the liquid to be aspirated when sucking the beads within capillary tubes 14. Controller 90 then sends a signal to syringe pump 64 to aspirate liquids into capillary tubes 14 for a time sufficient to capture the desired number of beads into each of capillary tubes 14. As with system 10 of FIG. 1, the number of captured beads may be controlled simply by controlling the length of wire 22 within lumen 20. After the specified time has elapsed, controller 90 sends a signal to remove capillary tubes 14 from reservoirs 88. Stage 80 is then moved to align capillary tubes 14 with a desired row of wells in plate 82. Manifold assembly 58 is then moved downward and controller 90 sends a signal to syringe pump 64 to irrigate liquids through capillary tubes 14 in order to expel the captured beads into the aligned wells 84 of plate 82. This process may be repeated as many times as desired in order to fill each of wells 84 of plate 82. Plate 82 may then be used in a biological screening or other process as is known in the art.
System 56 may also be used to transfer beads from one multiwell plate to another. For example, if one of wells 84 produced a positive result during the screening process, system 56 may be employed to individually remove the beads from that well and to place them into the wells of another multiwell plate so that further screening may occur to identify the compound synthesized onto the bead.
Referring now to FIGS. 4 and 5, one embodiment of a capillary tube of manifold assembly 92 will be described. Assembly 92 comprises a housing 94 to which are coupled a plurality of wire adjustment mechanisms 96 and a plurality of capillary tube assemblies 98. Housing 94 includes a plurality of ports 100 to permit manifold assembly 92 to be coupled to a syringe pump in a manner similar to that described with previous embodiments. As best shown in FIG. 6, housing 94 further includes a plurality of openings 102 into which wire adjustment mechanisms 96 may be inserted. Housing 94 further includes openings 104 for receiving capillary tube assemblies 98.
As best shown in FIGS. 5A and 6, wire adjustment mechanism 96 comprises a female/male adapter 106 that is insertable into opening 102. Insertable into adapter 106 is a sleeve 108 through which a wire 110 is inserted. Merely by way of example, female/male adapted 106 may be constructed of a stainless steel 10-32 to 114-28 adapter, commercially available from Upchurch Scientific. Sleeve 108 may be constructed of a PEEK tubing sleeve, 0.008 inch OD, 1.25 inch long, commercially available from Upchurch Scientific. Wire 110 may be obtained from California Fine Wire Company. Positioning of wire 110 through sleeve 108 is shown in greater detail in FIG. 5C. Sleeve 108 is configured such that wire 110 may slide through sleeve 108 to adjust the distance with which wire 110 extends into capillary tube assembly 98. The connection between sleeve 108 and wire 110 is also configured to provide a sufficient seal so that liquids introduced into or withdrawn from port 100 will not leak through sleeve 108. Further, the connection between sleeve 108 and adaptor 106 are configured to prevent any fluid leakage.
Capillary tube assembly 98 comprises a female/male adaptor 112 that is insertable into opening 104 of housing 94. Adaptor 112 is employed to couple a sleeve 114 to housing 94. Inserted into sleeve 114 is a length of tubing 116. Movably received within tubing 116 is wire 110.
Hence, by grasping wire 110 where it exits sleeve 108, a user may adjust the distance between a distal end 118 of wire 10 and a distal end 120 of tubing 116. This distance, in turn, defines a space where the beads will be captured. Hence, the number of beads that may be captured into tubing 116 may be adjusted simply by adjusting the location of wire 110 within tubing 116. Conveniently, the adjustment of wire 110 may occur manually or with the use of automated equipment.
The connection between sleeve 114 and tubing 116, as well as the connection between sleeve 114 and adapter 112, are sufficiently tight to prevent any fluid leakage. However, the spacing between wire 110 and tubing 116 is sufficient to permit the passage of fluids, such as when providing a vacuum for the bead picking process or positive pressure for fluid delivery or bead dispensing.
It will be appreciated that manifold assembly 92 may be constructed of a variety of material and to have a variety of sizes. Merely by way of example, housing 94 may be constructed of polypropylene, stainless steel, Teflon, or the like. Tubing 116 may be constructed of a polyimide capillary tube and may have, for example, a 198 μm inner diameter and a 236 μm outer diameter. Wire 110 may be constructed of tungsten and have a diameter of about 127 μm. Such dimensions are particularly useful with beads having a diameter in the range from about 120 μm to about 140 μm. However, it will be appreciated that the dimensions of manifold assembly 92 may be varied depending on the particular size of bead, the fluids involved, and the like.
Hence, in a bead picking operation, length of tubing 116 may be inserted into reservoirs having a plurality of beads. Fluids may then be aspirated through port 100 which causes a suction force to be created at distal end 112 of tubing 116. As such, one or more beads will be drawn into tubing 16 depending upon the positioning of wire 110 within tubing 116. Once the beads have been captured, manifold assembly 92 may be moved to another location and fluid under positive pressure introduced into port 110 to cause fluid to be irrigated out of tubing 116 to expel the captured beads.
Referring now to FIG. 7, one method for transferring beads as part of a biological screening process will be described. The process begins at step 122 where tubular member is placed into a reservoir having beads with the synthesized chemicals. A known number of beads is then aspirated into the tubular member as illustrated in step 124. The tubular member is then removed from the reservoir and placed over a well of a multiwell plate as shown in step 126. Positive pressure is introduced into the tubular member to expel the beads into one of the wells as illustrated in step 128. If more wells are to receive beads, the process reverts back to step 122 as illustrated in step 130. If all of the desired wells are filled with beads, the process proceeds to step 132 where the beads within the wells may be biologically screened according to processes known in the art. The wells are then evaluated for any positive outcomes as illustrated in step 134. If no positive outcomes are identified, the process ends at step 136. If one or more wells are identified, the process proceeds to step 138 and the tubular member may be introduced into the well to remove one or more of the beads. The bead may then be transferred to another well as illustrated in step 140. This process is repeated as many times as necessary in order to remove all of the beads as illustrated in step 142. Optionally, as shown in step 144, the transferred beads may be further screened as shown in step 144 to identify the compounds on the beads.
The invention has now been described in detail for purposes of clarity of understanding. However, it will be appreciated that certain changes and modifications may be practiced within the scope of the appended claims.
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|"Experience The Remarkable Difference . . . with Syringes Designed for Capillary Chromatography," SGE Solutions, Issue 2, 1994, p. 1.
|"Syringes for Capillary Crhomatography," SGE Solutions, Issue 3, 1993, p. 1.
|"The Ultimate Syringe for Dipensing Microlitre and Submicrolitre Volumes," SGE Analytical Syringes, Catalogue 1, 1987.
|"Type B-Do It Yourself Repair Kits," SGE Analytical Syringes, Catalogue 1, 1987, p. 1.
|"Type B—Do It Yourself Repair Kits," SGE Analytical Syringes, Catalogue 1, 1987, p. 1.
|Brochure, Genetix, The New Q Fill2.
|Brochure, Labsystems, 384 Technology.
|Brochure, Matrix Technologies Corporation, Platemate(TM).
|Brochure, Matrix Technologies Corporation, Platemate™.
|Brochure, Multidrop Dispenser.
|Brochure, Robbins, Scientific Corporation, HYDRA(TM)96.
|Brochure, Robbins, Scientific Corporation, HYDRA™96.
|Brochure, Skatron Instruments Inc., Embla 384.
|Brochure, Tecan, Genesis Logistics Workstation.
|Brochure, V&P Scientific, Inc., The Multi-Spense(TM) 384/96.
|Brochure, V&P Scientific, Inc., The Multi-Spense™ 384/96.
|Fischer Scientific Catalog (1988), pp. 884-885, 890.
|Kenichi et al. (1989), "Method for moving spherical carrier for antigen-antibody reaction," Patent Abstracts of Japan, vol. 13, No. 306 (P-897).
|Normie (Sep./Nov. 1996), article "System uses photonics for early tumor detection," in Biophotonics News, Biophotonics International, pp. 24-25.
|Citing Patent
|Filing date
|Publication date
|Feb 15, 2000
|May 3, 2005
|Applera Corporation
|Bead dispensing system
|Oct 25, 2002
|Jan 9, 2007
|Sequenom, Inc.
|Method and apparatus for parallel dispensing of defined volumes of solid particles
|Aug 31, 2004
|Aug 7, 2007
|Seiko Epson Corporation
|Device and method for manufacturing bead array, and method for detecting target substance
|Aug 2, 2002
|Mar 25, 2008
|Applera Corporation
|Bead dispensing system
|Jun 24, 2003
|Jun 10, 2008
|Applera Corporation
|Bead dispensing system
|May 11, 2007
|Oct 4, 2011
|Applied Biosystems Llc
|System and method for selective retrieval of support beads
|Jun 8, 2004
|Jul 3, 2012
|Biokit, S.A.
|Tapered cuvette and method of collecting magnetic particles
|Mar 8, 2012
|Jul 2, 2013
|Biokit, S.A.
|Tapered cuvette and method of collecting magnetic particles
|Aug 2, 2002
|Jan 30, 2003
|Vann Charles S.
|Bead dispensing system
|Oct 25, 2002
|Jun 19, 2003
|Elizabeth Nanthakumar
|Resin dispensing device
|Jun 24, 2003
|May 6, 2004
|Applera Corporation
|Bead dispensing system
|Aug 31, 2004
|Apr 14, 2005
|Fumio Takagi
|Device and method for manufacturing bead array, and method for detecting target substance
|Jan 25, 2006
|Jul 26, 2007
|Amir Porat
|Disposable vessels or tips having ultra-thin areas therein, and methods for manufacture of same
|May 2, 2007
|Nov 8, 2007
|Applera Corporation
|Variable volume dispenser and method
|May 11, 2007
|Nov 13, 2008
|Applera Corporation
|System and Method for Selective Retrieval of Support Beads
|Aug 20, 2013
|Dec 26, 2013
|Mts Medication Technologies, Inc.
|Automated solid pharmaceutical product packaging machine
|U.S. Classification
|422/534, 436/54, 422/68.1, 422/67, 436/180, 436/178
|International Classification
|Cooperative Classification
|European Classification
|Jul 20, 2000
Owner name: GLAXO WELLCOME INC., NORTH CAROLINA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:VELKOVSKA, SVETLANA;NG KERWIN;KEDAR, HAIM;REEL/FRAME:010964/0534
Effective date: 20000713
|Jun 4, 2002
Owner name: AFFYMAX, INC., CALIFORNIA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:SMITHKLINE BEECHAM CORPORATION;REEL/FRAME:012957/0178
Effective date: 20010814
Owner name: SMITHKLINE BEECHAM CORPORATION, PENNSYLVANIA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:GLAXO WELLCOME, INC.;REEL/FRAME:012957/0335
Effective date: 20010814
|Aug 13, 2004
Owner name: SMITHKLINE BEECHAM CORPORATION, PENNSYLVANIA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:AFFYMAX, INC.;REEL/FRAME:015061/0102
Effective date: 20030915
|May 17, 2006
|Maintenance fee reminder mailed
|Oct 30, 2006
|Lapse for failure to pay maintenance fees
|Dec 26, 2006
|Expired due to failure to pay maintenance fee
Effective date: 20061029


text:
login with
In this post do you mean something you wrap the baby in or snuggling up to go to sleep?
Well, either way, I love doing both! My little man is such a snuggler!! But I'm super careful with both of these. :)
Moms also like
About Jeanette Gartman
Born: Charleston, South Carolina
Current: Goose Creek, South Carolina
Birth: October 01
On Moms.com since: Feb 9, 2014
I'm a 23 year old single mum who loves Crocheting, baking from scratch, calligraphy, writing, reading, Medieval Times, HARRY POTTER, sword fighting, gardening, horse back riding, and cuddling with my little one! :D
All Recent Jeanette's Posts

subset: commoncrawl


text:
Butler Specialty Metalworks Fossil Stone Kaleidoscope Accent Table
$ 429.00
This eye-catching accent table is sure to be a topic of discussion. The kaleidoscope design on the top is made from fossil stone, and the geometric base is formed out of iron. Perfect for any modern home, this table combines style and functionality.
- Collection: Metalworks
- Finish: Metalworks
- Color: Multi-Color
- Shape: Square
- Style: Transitional
- Materials: Mahogany Wood, Plywood, Resin, Iron
- Dimensions: 14" w x 14" d x 26" h
- Cubes: 5.02
- Weight: 25 pounds


text:
I mentioned that last week wasn't a great week due to hubby having an epic three day celebration for his birthday. After all of that indulging I followed up with redoing the carb detox for 2.5 days (ended up having veggies with dinner on the third day). That seems to have undone my sins of the weekend. My weight at my official weigh in last week was 63.4kgs. Out of interest, I weighed myself again after the weekend and found that I had gone back up to 64.4kgs. I know that a lot of that gain would have been fluid as, for each gram of carbs you body stores, it also stores 3g water. For my weigh in this week, I had lost all of that extra gained over the weekend plus another 300g.
My results:
Weigh In:
Starting weight: 72.5kgs
Weight after week 1: 69.4kgs
Starting weight: 72.5kgs
Weight after week 1: 69.4kgs
Weight after week 2: 68.7kgs
Weight after week 3: 68.1kgs
Weight after week 4: 67.2kgs
Weight after week 5: 66.7kgs
Weight after week 6: 65.4kgs
Weight after week 7: 65.4kgs
Weight after week 8: 64.7kgs
Weight after week 9: 64.1kgs
Weight after week 10: 63.4kgs
Weight after week 11: 63.1kgs
Loss in last week = -0.3kgs
Total weight loss: 9.4kgs
I had a great week but then not the best weekend. I have to admit that I am finding weekends hard. Hubby is currently completing his CFP so the kids and I have to make ourselves scarce one day of the weekend so he can study. Mum is a teacher and her school was holding an international fair so I took the kids along. I managed to stay compliant with my lunch by having chicken satay sticks. But then we didn't get home until 4.30pm, I was exhausted and hadn't gotten anything out of the freezer for dinner, so we ordered pizza in. The following quote has been floating around the Internet forever and I recently saw it on a Bodytrim follower's Instagram:
Fail to prepare then prepare to fail
It is so true. I failed to organise anything for dinner so we ended up having pizza. I need to find some takeaway like compliant recipes that I can cook in advance and store in the freezer. I do the majority of meal preparation in our family so when the weekend comes around I just want to have a weekend off; I don't want to have to think about what to feed everyone then go about doing it. Sunday was always going to be my day off as it was Father's Day and we had a BBQ planned (with brownies for dessert!). I had prepared for that which is why Saturday was a failure.
Once I reach the weight maintenance phase I can choose from either having carbs with breakfast and lunch I and then taking just one free day a week or sticking with only carbs at breakfast and then getting two free days a week. I actually haven't minded not having carbs after breakfast so I think I will take the second option. I find it easy sticking to the way of eating during the week but come weekend I want more flexibility.
I now have 1.1kgs left to go to reach my target and 3 weeks to do so, so I am pretty sure that I am going to be successful. I'm still feeling flabby around the middle so perhaps will go for a little bit longer - I will reassess when I get to 62kgs.
What did you do for Fathers Day? I have to confess that I made all of the dads in my life this ridiculously good; ridiculously non Bodytrim compliant 5 minute fudge. I ate a little bit on the weekend but have been good during the week. I just have to hope hubby doesn't get through it all before my free day this weekend because I want some moooorrrre!
(just a heads up, in case you missed it, but I have 10 bottles of Orly nail polish up for grabs on the blog for one lucky winner. Enter here)
Follow the rest of my journey here


text:
First of all:
I made a new video!
This is my announcement from my profile:
"2.) This is a homemade for-fun promo I did the other day for the movie. It's alot better than the Ferb tribute. Even with a cruddy program like windows movie maker (never use 'cause it sucks! But it's all I've got.) , I've still improved in learning how to make a fairly good video. It looks pretty cool if you ask me, I'm not gonna lie ;) . Click here to watch! I invite anyone else who enjoys making videos for fun to also make a homemade promo/commercial thing! It would be fun! Maybe we could make some sort of contest out of it or something! But either way I wanna see your homemade promo! Send the link of your video to my talk page [[]]here. Peace!
PS If you are not able to watch the video on the link above, try this one ---> http://a32.video2.blip.tv/11890008949268/ILovePhineasAndFerb-HomemadeATSDPromo731.wmv?brs=1298&bri=5.1 "
If anyone is interested, see my talk page for more info and a place to share your creation here: http://phineasandferb.wikia.com/wiki/User_talk:ILovePhineasAndFerb#Your_Homemade_ATSD_Promos
I've been trying to get some light shone on this subject for some help, but, well, yeah. Just read the latest entry from my talk page:
"Ummm, I know I haven't been on for a while, and my "My fanfic report (Tara Moves to Danville)" blog hasn't been edited at all in an even longer time, but how do I reactivate it? I did not appreciate that by the way. I'm not against having blogs become inactive, but some permission and consent from the user or even a notice of expiration would be nice. Anyway, if someone could help me to do that and let me know about any new rules, admin regulations, and new wiki format navigations I would be most grateful. I'm still not completely familliar with the new format yet, or even changes with the way things are being run. If I can't reactivate the blog, what can I do to keep the gist of it continually going and able to be there any time? I personally would like to be able to have control over my own blogs, I was suprised when I couldn't even post anything on my own blog. It dosen't seem to be a setting that I can turn on and off which is kinda concerning to me. Yeah, so thanks! =D
~TMTD progress updates~
I'll have to add a blog post about how the story is comming later and maybe even a few previews, but let's just say, I think the final outcome might be almost as long as "Finding Dad". Good stuff, good stuff. So far I have the beginning done and the ending done, now I just need to connect the two with story in the middle. Yes, I haven't abandoned Tara Moves to Danville. Even when it's done though, I will still need to edit it and do any final proof reading before I post the final story on fanfiction.net and the fanon. But still, I am still not sure when it will be released. In hindsigtht, I should probably finish a story before I start advertising. Oh well, live and learn.^^ I think it's gonna be well worth the wait (especially for you hardcore romantics out there), just be patient. PEACE! ILovePhineasAndFerb =) 23:58, June 29, 2011 (UTC) "
That was from last month, but because it was on my talk page, no one really knew about it. I think the best way to have info set out and seen is through the blogs. That's where everyone goes to first.
Please don't delete my Hunger Games userboxes! I didn't know if that would interfere with the rules or not, but I figured, if people can have the mon goose dog (gir from Invader Zim) boxes, then I could do that.
Well, technicly I guess this really isn't a whole lot of information given at a time. It all just felt really random, so I didn't know what to call it. Before I typed this up, I thought I had alot more that needed to be said, but I've probably have just gone blank. I will add comments of things on my mind as they come back to me I guess...
Oooh, I almost forgot. If you see my video and want to review it, here would be the place to do so as well.
peace! ILovePhineasAndFerb =) 06:38, July 30, 2011 (UTC)


text:
Teares Primitivos
Prof° Mário Scaglione
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Tear primitivo Vertical
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Tear Esloveno com Rolo
Tear manual - Tailândia
Tear Vertical
Teares Modernos
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Teares Primitivos
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text:
Portable Buildings local companies in Missouri MO- Displaying all 16
BIG Enterprises, Inc. serving Missouri
We are a custom manufacturer. We can custom manufacture every booth according to your needs and site requirements.
Products: Buildings: portable; buildings: mobile or portable; buildings: portable, metal; booths; booths: bullet proof; booths: cashier; booths: information; booths: parking attendants'; booths: parking, kiosk; booths: prefabricated movable; booths: security; booths: ticket; booths: toll; buildings: bullet resistant; buildings: modular; buildings:..
Par-Kut International Inc. serving Missouri
Portable Buildings can be assembled on site or transported to the needed locations whole. Sizes vary from single room to office systems. Portable buildings are ideal for construction sites and docks.
Products: Buildings: portable; buildings: mobile or portable; buildings: portable, metal; booths; booths: bullet proof; booths: cashier; booths: parking attendants'; booths: prefabricated movable; booths: security; booths: ticket; booths: toll; buildings: aluminum; buildings: bullet resistant; buildings: communications; buildings: factory;..
Emerson Process Management Hazelwood, MO
Products: Buildings: portable; control panels: electric; engineering services; engineering services: electrical or..
Essex Pb & R Corp Saint Louis, MO
Products: Buildings: portable; aircraft parts & auxiliary equipment: oxygen systems; breathing apparatus;..
Marshfield Portable Building Marshfield, MO
Products: Buildings: portable; buildings & components: prefabricated metal; buildings: factory; buildings:..
Show Me Fabrication Sedalia, MO
Products: Buildings: portable; carts: industrial; food service equipment; glass specialties; housings; trailers;..
PortaFab Chesterfield, MO
Products: Buildings: portable; buildings: mobile or portable; buildings: portable, metal; air showers; blast..
Porta-Fab Corp. Chesterfield, MO
Products: Buildings: portable; booths; booths: bullet proof; booths: cashier; booths: exhibition; booths:..
B & B Storage Leasing LLC Brookline Station, MO
Products: Buildings: portable;
Cedarridge Portable Buildings Springfield, MO
Products: Buildings: portable; buildings: prefabricated; buildings: prefabricated wood; buildings: storage;..
Classic Equine Equip Inc Fredericktown, MO
Products: Buildings: portable; buildings & components: prefabricated metal; buildings: prefabricated, wood; panels: building
Freeburg Hardwood Products Inc Freeburg, MO
Products: Buildings: portable;
Hilkemeyer General Store Freeburg, MO
Products: Buildings: portable; barns; buildings; buildings: prefabricated wood; coil handling & feeding equipment;..
Morton Buildings Inc Mexico, MO
Products: Buildings: portable; buildings: prefabricated wood; buildings: prefabricated, wood; construction..
Sentinel Industries Inc Ashland, MO
Products: Buildings: portable; buildings; buildings: farm, prefabricated or portable, wood; buildings:..
Starrco Modular Office Systems Maryland Heights, MO
Products: Buildings: portable; booths: bullet proof; buildings; buildings: air supported; buildings: aluminum;..
Top quality manufacturers serving Missouri MO have been highlighted in this comprehensive source of industrial information. A broad range of BUILDINGS: PORTABLE manufacturers has been compiled in this industrial directory designed to provide information on leading, quality oriented manufacturers serving Missouri MO.
Cities being served include: Arnold, Ballwin, Belton, Blue Springs, Branson, Bridgeton, Camdenton, Cape Girardeau, Carthage, Chesterfield, Columbia, Earth City, Farmington, Fenton, Florissant, Grandview, Hannibal, Hazelwood, Independence, Jackson, Jefferson City, Joplin, Kansas City, Lebanon, Lees Summit, Liberty, Maryland Heights, Mexico, Moberly, Monett, Neosho, Nixa, O Fallon, Ozark, Pacific, Perryville, Poplar Bluff, Rolla, Saint Charles, Saint Joseph, Saint Louis, Saint Peters, Sedalia, Sikeston, Springfield, Sullivan, Union, Washington, Wentzville, West Plains.
Area codes being served include: 314, 417, 573, 636, 660, 816.
Portable Buildings local companies in Missouri MO - Displaying all 16
Portable Buildings - Companies Serving:All States
Alabama AL (16)
Alaska AK (6)
Arizona AZ (5)
Arkansas AR (10)
California CA (47)
Colorado CO (16)
Connecticut CT (6)
Delaware DE (4)
Florida FL (29)
Georgia GA (15)
Hawaii HI (3)
Illinois IL (18)
Indiana IN (15)
Iowa IA (7)
Kansas KS (7)
Kentucky KY (8)
Louisiana LA (21)
Maine ME (5)
Maryland MD (8)
Massachusetts MA (8)
Michigan MI (9)
Minnesota MN (5)
Mississippi MS (8)
Missouri MO (16)
Montana MT (3)
Nebraska NE (7)
Nevada NV (4)
New Hampshire NH (3)
New Jersey NJ (8)
New Mexico NM (5)
New York NY (17)
North Carolina NC (16)
North Dakota ND (3)
Ohio OH (7)
Oklahoma OK (16)
Oregon OR (12)
Pennsylvania PA (24)
South Carolina SC (11)
South Dakota SD (4)
Tennessee TN (12)
Texas TX (53)
Utah UT (3)
Virginia VA (13)
Washington WA (11)
West Virginia WV (5)
Wisconsin WI (10)


text:
Lamia Balafrej
Assistant Professor of Art
B.A., M.A., Ecole Normale Supérieure (Paris) and University of Paris; Ph.D., University of Provence
Art historian working on the art and architecture of the Islamic world and their interweaving with global art history.
Rebecca Bedell
Associate Professor of Art
B.A., Wellesley College; M.A., Ph.D., Yale University
Wellesley alumna; research explores the relations between art and science, and the place of sentimentalism in American art.
Patricia Gray Berman
Theodora L. and Stanley H. Feldberg Professor of Art
B.A., Hampshire College; M.A., Ph.D., New York University
Art historian specializing in the art and visual culture of the late 19th-, 20th-, and 21st centuries.
Margaret D. Carroll
Professor of Art
B.A., Barnard College; A.M., Ph.D., Harvard University
Primary research in the field of 16th- and 17th-century Dutch and Flemish painting, with specialized work on Hieronymus Bosch, Pieter Bruegel the Elder, Rubens, and Rembrandt.
Kimberly Cassibry
Assistant Professor of Art
B.A., Louisiana State University (Baton Rouge); M.A., University of Texas (Austin); Ph.D., University of California (Berkeley)
Art historian focusing on cross-cultural connections in the ancient Mediterranean
Alice T. Friedman
Grace Slack McNeil Professor of American Art; Professor of Art
A.B., Radcliffe College; M.Phil., University of London; Ph.D., Harvard University
Interested in building an interdisciplinary, feminist approach to architecture through research, teaching, and public education.
Nikki A. Greene
Assistant Professor of Art
B.A., Wesleyan University; M.A., Ph.D., University of Delaware
Art historian examining African and African American identities, music, the body, and feminism in 20th century and contemporary art.
Heping Liu
Associate Professor of Art
B.A., Guangzhou Institute of Foreign Languages (China); M.A., Southern Methodist University; M.A., M.Phil., Ph.D., Yale University
Asian art historian and specialist in Chinese painting of the Song dynasty (960-1279).
Barbara Lynn-Davis
Lecturer in Art
B.A., Brown University; M.A., Williams College; Ph.D., Princeton University
Research focuses on the city of Venice, Renaissance to the eighteenth-century.
Martha McNamara
Director of New England Arts & Architecture Program
Expert in New England art and architecture. Co-directs the architecture program.
Jacqueline Marie Musacchio
Professor of Art
B.A., Wellesley College; M.A., Ph.D., Princeton University
Specialist in Italian Renaissance and Baroque art.
James Oles
Senior Lecturer in Art
B.A., Yale University; J.D., University of Virginia; M. Phil., Ph.D., Yale University
Specialist in Latin American art, focusing on modern Mexican art and architecture, through museum as well as academic projects.
Liza Oliver
Assistant Professor of Art
B.A., University of North Florida; M.A., University of South Florida; M.A., Ph.D., Northwestern University
Art historian focusing on 18th- and 19th-century Europe and South Asia, colonialism, Indian Ocean trade, and intersections of art and science.
Ruth R. Rogers
Curator of Special Collections
B.A., Middlebury College; M.S., Simmons College
Book and manuscript studies, text and image, contemporary artists' books.


text:
1) Call to Order/Roll Call/Pledge of Allegiance
2) Approval of the Agenda
3) Good of the Order
a) Recognition and promotion of Police Reserve Officer Darren Skeries to the appointed position of Reserve Captain
4) Council Member Comments
a) Board, Committee Commission Update and Information
5) Approval of the Minutes:
a) April 6, 2015 Regular Council Meeting
6) Public Comment. This is an opportunity for any citizen to come forward and discuss any item that is not listed on the Agenda. (Please make your remarks from the podium and limit your presentation to no more than 5 minutes.)
7) Public Hearing.
a) Proposal to Issue Not to Exceed $8,000,000.00 Aggregate Principal Amount of City's Revenue Bonds (Childserve Project) Series 2015
b) Proposed Plans, Specs, Form of Contract and Estimate of Cost - Hickman Road Streetscape Improvements Project
8) Mayoral Proclamation
a) Earth Day
9) Approval of the Consent Agenda.
All items listed below are considered routine by the City Council and will be enacted by one motion. There will be no separate discussion of these items unless a Council Member or citizen so requests, in which event the item will be removed from the Consent Agenda and considered separately.
a) Claims & Payroll
b) March Treasurer's Report
10) Consideration Of Ordinance No. 15-02, An Ordinance Adopting The "Code of Ordinances of The City of Windsor Heights, Iowa" – (2nd Reading)
11) Consideration of Approval for Setting a Date for a Public Hearing, Budget Amendment – (May 18, 2015)
12) Consideration of Approval, Revision to Employee Personnel Policy 13.1 Public Safety Building Work Hours
13) Consideration of Revised Job Description, Parks and Special Events Coordinator
14) Consideration of Resolution No. 15-0432, A Resolution Adopting Plans, Specifications, Form of Contract and Estimate of Cost
15) Consideration of Resolution No. 15-0433, A Resolution to Proceed With the Issuance and Sale of Revenue and Refunding Bonds (Childserve Project), Series 2015A, In An Amount Not to Exceed $8,000,000
16) Consideration of Resolution No. 15-0434, A Resolution Authorizing The Issuance, Execution, Delivery and Sale of Revenue and Refunding Bonds (Childserve Inc. Project), Series 2015 In The Aggregate Principal Amount Not To Exceed $8,000,000, of The City of Windsor Heights, Iowa, The Execution and Delivery of A Loan Agreement and Second Supplemental Indenture of Trust, and Other Matters Relating Thereto
17) Consideration of Resolution No. 15-0435, A Resolution Authorizing And Approving Entering Into A Letter of Intent With The City of Clive Iowa for the Investigation and Analysis of A Potential Consolidation of Fire / EMS Departments
18) Administrator Report
19) Mayor's Inquiries
20) Adjourn to 6:00pm on Monday May 4 at Windsor Heights City Hall


text: Case | HBS Case Collection | April 1995 (Revised May 1995) Kraft General Foods: The Merger (B) David J. Collis and Elizabeth Wynne Johnson Discusses the recent decision to blend the previously separate Kraft and General Foods units into one operating company with a focus on the creation of a single massive sales force. Keywords: Mergers and Acquisitions; Salesforce Management; Corporate Strategy; Consumer Products Industry; Food and Beverage Industry; Citation:Collis, David J., and Elizabeth Wynne Johnson. "Kraft General Foods: The Merger (B)." Harvard Business School Case 795-153, April 1995. (Revised May 1995.) View Details CiteView DetailsEducatorsPurchase Related


text: Fine jewelry shopping just got more exciting! Make the most of Angara's Green Monday Deals to get your hands on exquisitely crafted jewelry, including rings, earrings, pendants and bracelets, at amazing prices. Whether you are looking to expand your collection or gift something unique to a special someone, what could be better than Angara's Green Monday Deals to satiate your thirst from some bling? Cheers to shopping your heart out!


text:
Planning Commission
Planning Commission has reported an advertisement of Planning Commission Recruitment 2017-2018 for various posts.Interested and eligible candidates may apply by filling application form in prescribed format.
|Planning Commission
Organization Name:
Planning Commission
Entire Information of Planning Commission :
S.No Name of the Post No of Posts
1 Young Professional 01
2 Consultant 01
Organization Name:
Educational Qualification:
Those who want to apply for the above mentioned posts must hold such relevant degree from recognized university/ Institute
Master's Degree in Economics/Statistics/ Econometrics/ Management
Knowledge of Statistical application such as R, Strata and Office productivity applications like Spread-sheet-mail client.
Graduate in "health- related discipline" with post graduate in Public health/Community medicine/ Management
Experience: should have relevant experience in appropriate field as mentioned below:
Ph.D or Research experience.
Experience in collection, Compilation and analysis of data.
Professional experience in Planning/execution/ evaluation of projects or academic work in the health sector.
Track record of Publications in peer reviewed journals
Age Criteria:
Post Name Age Limit
Young Professional 40 years
Consultant 65 years
Selection Procedure:
will be selected based on the performance in:
Screening Test
Written Test
Salary Criteria:
Selected applicants will get good amount of pay scale according to the posts as stated below:
Post Name Pay Scale
Young Professional Rs. 25,000 to 40,000/- per month
Consultant Rs. 40,000 to 70,000/- per month
How to apply
Those who are fulfilling the eligibility conditions may apply by sending their latest curriculum vitae and are required to attach all essentials details along with two recommendation letter and forward it to the below address.
"Smt. Manisha Verma,
Section Officer,
Health & Family Welfare Division,
Planning Commission,
Room No. 526-C, Yojana Bhavan,
Sansad Marg, New Delhi – 110001".
Important Dates:
Last date to send application form: 28th March 2017-2018 on or before 5.30 PM
For more details you may go through the
Official Advertisement for Young Professional
Official Notification for Consultant Click Here…


text:
Amelia is learning to love humans and allow them to love her. Her previous experiences were not positive. But we are starting to see her blossom into a happy dog. She needs time to understand that not all humans are bad. This sweet little nugget loves to follow you around the house and has even started to enjoy snuggling.
Amelia is great with other dogs. And will soon be able to fly into your heart. We are looking for a family that will show her patience and love.
Her adoption fee is $250 and an application can be completed at www.onmywayhomerescue.com
Thank You!
Your message has been sent to On My Way Home Rescue.
You'll receive a copy, too, at to help you keep track of which pets you've inquired about, and which shelters and rescues you've emailed.
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These angel eye will blow away competition...
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Originally Posted by
So the x5 pic ha the Orion's in it? Which model were they? What color was it in the description on the site or wherever you go them? That is more of the color I am looking for. I guess my next step after that is how to wire it. The baking I have seen before and willing to do. I will post a pic later on how mine are currently. They may appear bright, but are very deceiving. In the day you cannot see them. Can you see the Orion's then? I didn't want the F10 just the similar color and for sure the brightness. They around $180 a pair?
Yep my E53 X5 has "Orion" halo rings in the headlamps. your E39 probably has the same electrical circuit as my E53, I tapped the main light switch to drive a relay to turn the halo-rings. If you paralell the park light at the headlamp then the halo ring will blink with tirning signal, not the effect I want.
BTW, you can buy the rings here:
not sure why anyone would pay more than $50 for all four rings, these are made by stringing cheap white LED lights to make up a ring that can be powered @12V. I think I know who wants to charge you $180 for them
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Best Of :: Arts & Entertainment
It's hard to imagine how the original owner of Frank Lloyd Wright's Hollyhock House, the oil heiress and renegade Aline Barnsdall, could find it intolerable. She practically forced the city to take the 5,000-square-foot Mayan-esque compound on Olive Hill, with its surrounding moat and central courtyard and interior river, off her hands. The city finally did, in 1927 — and it did a shoddy job of keeping up with the property (those Wrights are a bitch to maintain), at least until recent years. A meticulous, $4.4 million restoration from 2010 until February 2015 returned much of the house, now part of Barnsdall Art Park, to its original function and intent. The egomaniacal Wright and the eccentric Barnsdall had their aesthetic differences, but there's no denying that Wright was deeply inspired by his first house in L.A., having stylistically left behind the flat and frigid prairies of the Midwest for the dramatic and exotic influences of Southern California. In a way, the seed of modern architecture was planted there on Olive Hill; when Wright couldn't devote enough attention to the project, he coaxed his colleague from Chicago, Austrian architect Rudolph Schindler, to come west. Schindler in turn brought on his friend from the motherland, Richard Neutra. Thanks to the Hollyhock, the rest is history.
Comedy writer Jason Shapiro started the Twitter feed @LosFelizDaycare in 2013, and you would have thought it would be old hat at this point, but there is no shortage of fodder for satirizing young, educated, progressive, Angeleno parents. The feed spoofs not only their child-rearing styles but also their other fixations, such as food trends ("Truffle macaroni art was a huge success today"), mindfulness ("Ramen (3) will only eat white foods but we're letting her live her truth for now."), debates over gender in pop culture ("Hmm we like that the new Ghostbusters cast is all female but we can't be supporting Judeo-Christian afterlife propaganda") and, of course, vaccination ("Java (3) is giving a talk on vaccine paranoia vs. vaccine hesitancy tomorrow. Time is meaningless so whenever you come will be the right time.") It's fun to imagine a daycare in which Intelligentsia shows up in a "Free Adnan" T-shirt and Wren calls Linus a "slacktivist" over the sounds of Sleater-Kinney before everyone sits down to watch Pussy Riot's House of Cards appearance together to avoid spoilers.
Photographer Kate Rentz's Instagram feed will make you want to put down your phone and grab a sleeping bag. The Los Angeles–based photographer is always outdoors: hiking in the mountains, camping in the forests, twisting through canyons or even just playing Monopoly in the park. Snap a shot of her dinner? Not unless it's a picnic basket. Rentz's quest to merit her favorite hashtag —#LiveAuthentic — has taken her as far as backpacking on an Argentine glacier, and her nature portraits of her husband, music video director Isaac Rentz, and their dog Maggie are both stunning and cozy. But it's her pictures of California that make her our favorite Instagrammer. Not only do her photos capture the ecological diversity of our state, they urge you to get in the car and chase her trail. Just check out the comments: "Where is that?" "How close?" and "Take me!"instagram.com/katerentz
As an L.A.-based musician of complex talents and singular taste with his own band, The One AM Radio, Hrishikesh Hirway understands the amount of finesse and perfectionism that goes into crafting fantastic songs. With his Radiotopia-powered podcast, Song Exploder, he takes that understanding to interviews with other music makers — some of whom are of the household-name variety — and draws out the best parts and thoughts. By doing all of the hard work of chasing down his favorite songmakers, conversing with them at length and — most importantly — filing his conversations down to their essential pieces, he ends up with a tight but well-tuned 13 to 15 minutes about a single song. All of his own effort cuts out the prosaic (though often necessary to the process) prattle that lesser music podcasts force their listeners to endure. Given that he's been featured in Wired and the AV Club and has featured acts like U2, Death Cab for Cutie and Best Coast, it's doubtful he'll have to do much more chasing.songexploder.net
Suffice to say, Dave Ross is a funny guy. His storytelling and self-deprecating entr'actes to his own late, great Holy Fuck free comedy show in downtown L.A. were perfect morsels of what the kids used to call "alt comedy." Even better, his superb taste in other comedians, from nobodies to marquee acts, brought together the kind of talent that average schmoes would have lined up to pay for at mainstage clubs. Ross takes all that and, with new co-host Anna Seregina, puts it into the magic of Internet streams. The show, operating under Nerdist's banner, focuses on insecurities rather than merely yuks, and, unlike certain garage-based hosts' nasal chattering, Ross' gravelly baritone has a soothing effect that facilitates his guests' self-dissection. While he's not drawing commanders-in-chief (yet), his roster pulls in the kinds of folks on the edge of L.A.'s cultural fabric whom you'll be posting about and retweeting in the years to come.nerdist.com/podcasts/terrified-channel
The setup is simple — six comedians rhyme over a beat, competing with one another for audience votes — but the results are endlessly varied. Every second Tuesday of the month, stand-up and improv comic Eliza Skinner, with the beatboxing assistance of Joshua Silverstein, hosts Turnt Up!, a rap battle that features your favorite local stand-ups leaving their scripts and set lists behind to risk humiliation in the freestyling ring. The audience rewards cleverness and fearlessness over insults and the show has become so popular that professional rappers — from Busdriver and Open Mike Eagle to comedic rhymer Wayne Brady — occasionally pop in, taking the stage at the end to play around with the winning comic. No matter the surprise star power on any night, though, the most impressive rhymes typically come from Skinner herself, who opens each show by spinning an audience suggestion into an impromptu track.


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Peak performance: Following in James Bond and Sylvester Stallone's macho footsteps on the Dolomite mountain range
Some of the world's most famous action heroes have filmed scenes of derring-do in the Dolomite mountain range, part of the northern Italian Alps. From Bond to Stallone, they have flexed their muscles on these challenging slopes.
Today I am following in their macho footsteps - tackling a two-hour via ferrata climb up Col dei Bos (Hill of Cows), whose peak stands at 2559 metres. My guide is the nimble Mario, who has scaled Everest and K2, and was a climbing adviser on Stallone's Cliffhanger, filmed here. (Mario remembers the Hollywood star as very short, unfeasibly muscle-bound and a bit of a scaredy-cat).
Living the high life: Laura gets ready to tackle the two-hour via ferrata climb up Col dei Bos
Via ferratas are fixed climbing routes created so that soldiers in the First World War could haul up supplies to tunnels and trenches. The limestone peaks and valleys around the region's main town of Cortina d'Ampezzo formed a front between Italian and Austrian soldiers – the Italians eventually prevailed in 1917 and Cortina, formerly Austrian, became part of Italy. Many of the mountain dug-outs have been preserved as outdoor museums.
The via ferratas, meaning iron routes, consist of rungs hammered into the rock to create a ladder. A steel cable runs alongside and climbers clip onto it to secure themselves. While the Dolomites were their birthplace, via ferratas have now been set up worldwide to enable climbers of all levels to attempt difficult ascents.
My 'intermediate' route, called Via Ferrata degli Alpini, begins with a sheer vertical stretch and I find myself scrabbling clumsily. I'm sure even wimpy old Sly Stallone didn't wail 'I'm stuck!' and swear tearfully at bruised knees. I have climbed a bit, but would still class myself as a novice…
Action man: 'Scaredy cat' Sylvester Stallone filmed Cliffhanger in the Dolomite mountain range
I am attached to Mario and two fellow climbers by a rope (an extra precaution for beginners). On my harness is a length of material with a clip on the end. Each time I progress upwards, I clip above the next bolt on the metal cable. I use the rungs to help me climb, but they are quite far apart (I'm short) and I need to find foot and handholds too.
After a difficult first half hour, the climb gets easier and we stop at the natural platforms en route, looking out over jagged spires and smooth plateaus. The 18 peaks of the Dolomites are a UNESCO World Heritage Site – they have borne witness to the evolution of life and landscape for more than 250 million years.
Reaching the end of our 300m climb is a triumphant moment. We whoop, high five, breathe, take in the views and head for a well-deserved lunch at Rifugio Laguzoi.
There are 56 refuges in the region, which are largely Alpine-style wooden chalets offering good food and sometimes beds for the night. I tuck in to Laguzoi's famous carrot gnocchi before heading outside for a photo on the promontory where Stallone filmed Cliffhanger's opening scene.
Cliff hanger: Laura holds on for dear life as she tackles a sheer vertical stretch on the Via Ferrata degli Alpini's 'intermediate' route
Well-known as a high-end ski-resort favoured by celebrities including George Clooney, Cortina d'Ampezzo and the surrounding area is increasingly becoming an all-year-round destination. From June to October, snow gives way to meadows of wild flowers and a network of walking, hiking, biking and climbing routes.
I spend a morning with mountain bike guide Fabio, who takes me along Cortina's disused railway track, now a 30km cycle route through forests of larch and pine.
Fabio's family are 'Ampezzani', the name given to the region's longest-serving residents. His ancestors set up home here in the 13th century, first as farmers, then as ironmongers. Fabio is a ski instructor in winter time and is obscenely fit for 51. A father of three, whose wife is a florist, he can't imagine living anywhere else. 'My life is here,' he says.
We make our way up and down pine-needle paths and shale, taking in the ruin of an 8th-century castle and dipping our feet into an ice-cold mountain stream.
Mountain refuge: Laura stayed the night in family-run Rifugio Averau, which offers good home-cooked food and comfy dorms
While Cortina is definitely a draw for the adventurous, there is plenty to do if you prefer more gentle activity. The town itself feels more Austrian than Italian, with carved wooden balconies and baskets of geraniums. Designer brands are wedged between the outdoor-wear shops, and in the evenings well-groomed locals and visitors sip Aperol spritzers. My spacious room at the Hotel Franceschi looked out over mountain peaks and was a five-minute walk from Pasticceria Alvera, brilliant for morning croissants or afternoon cake.
There are easy walks or drives to surrounding villages and lakes, and I'd recommended lunch or dinner at Il Brite de Larieto, a working farm and restaurant two kilometres out of town. Everything on the menu is made here, from ham and cheese to fennel-seed bread and traditional dumplings.
The region's mountain refuges are also reachable without hiking for miles as lots of them have cable cars which take you close by.
I stayed the night in family-run Rifugio Averau, with good home-cooked food and comfy dorms, where a dramatic midnight thunderstorm ricocheted around the mountains.
Celebrity haunt: Cortina d'Ampezzo and the surrounding area is increasingly becoming an all-year-round destination
In the morning I walked 8km along one of the well-marked hiking routes for lunch at Rifugio Scoiattoli, overlooking the towering Cinque Torrei rock formations. My plate of violette pasta with thyme, pine nuts, porcini mushrooms and pancetta was the most memorable dish of the trip. Scoiattoli's huge terrace was scattered with beanbags and sheepskin rugs – and in winter they fire up an outdoor hot-tub.
Back in Cortina on my final evening, I ask a local where to go for an aperitivo. The suave gent in his sixties with a jumper casually thrown around his shoulders directs me to Open Space – a glass-fronted bar on the town's main street, Corso d'Italia.
He joins me for a chat, flattering my sporting ambitions by asking me if I'm in town to take part in the ultra marathon. I later discover that he was the stunt double for Roger Moore in 'For Your Eyes Only' – the ski-chase scene was filmed here. What better way to end a holiday than to be chatted up by James Bond?
Travel facts
British Airways flies from London Gatwick to Venice Marco Polo (www.britishairways.com) and Monarch flies from Birmingham International to Venice Marco Polo (www.monarch.co.uk). Flight prices vary according to when you book and the timings.
The two-hour coach transfer from Venice to Cortina with Cortina Express costs €22 one way.
Half-board rates at the four-star Francheschi Park Hotel cost from €75 per person per day: www.franceschiparkhotel.com
Half-board in dormitory accommodation at Rifugio Averau costs from €55 per person per day.
Cortina d'Ampezzo is one of 12 select resorts belonging to the European association 'Best of the Alps': www.bestofthealps.com
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The majority of installations are exposed to external forces such as vibration and variable dynamic loads. Weather conditions, such as wind and temperature changes, can also affect a bolted connection.
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The "No Need For Retightening" certification course aims to ensure the installation of a safe and secure bolted connection. In order to be certified you must go through all modules and pass the final test.
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text: Nuveen Investments, a leading global provider of investment services to institutions and high-net-worth investors, today announced that Nuveen Asset Management (Nuveen) has assumed the role of investment adviser for the NASDAQ Premium Income & Growth Fund Inc. (NASDAQ: QQQX) previously managed by IQ Investment Advisors LLC (IQ Advisors). The fund's shareholders approved this change on October 22, 2010. This development completes the transition of five closed-end funds previously managed by IQ Advisors to Nuveen. As previously announced on October 8, 2010, the four additional funds include: · Dow 30 SM Premium & Dividend Income Fund Inc. (NYSE: DPD) · Dow 30 SM Enhanced Premium & Income Fund Inc. (NYSE:DPO) · Global Income & Currency Fund (NYSE:GCF) · MLP & Strategic Equity Fund Inc. (NYSE:MTP) The NASDAQ Premium Income & Growth Fund Inc. will continue to be managed by the same investment teams with Nuveen affiliate Nuveen HydePark Group, LLC (HydePark) continuing as sub-adviser to the fund. Nuveen Investments provides high quality investment services designed to help secure the long-term goals of institutions and high net worth investors as well as the consultants and financial advisors who serve them. Nuveen Investments markets its growing range of specialized investment solutions under the high-quality brands of NWQ, Nuveen, Santa Barbara, Symphony, Tradewinds, Winslow Capital and Nuveen HydePark. In total, the Company managed more than $160 billion as of September 30, 2010. For more information, please visit the Nuveen Investments website at www.nuveen.com.


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Think of all the prison flicks you've seen. Now, think about the wardens in those movies.
The men who run the penitentiaries in The Shawshank Redemption, The Longest Yard and Escape from Alcatraz tend to be nasty, cynical and vindictive.
Julie Cobb, BA'87, is no pushover, but she has a downright sunny disposition compared to those guys.
Cobb is the warden of Archambault Institution in Sainte-Anne-des-Plaines. It wasn't the career she had in mind when she finished her McGill degree in psychology. Anglophones were underrepresented in federal government jobs in Quebec at the time and her boyfriend's father was aware of attempts being made to rectify that. He urged her to send in an application. Cobb landed a position in Correctional Services Canada.
"I fell into the job by accident," she recalls. If she was initially surprised to find herself working for Correctional Services, she was even more surprised when she realized how much she enjoyed it.
Last June, at the official change of command ceremony during which she became Archambault's new warden, Cobb told the crowd, "I've had the greatest career."
She worked her way up. She has been a correctional officer, a parole officer, a unit coordinator and the deputy warden of the Joliette Institution for Women.
"I never have two days that are the same – that's what makes the job so interesting," says Cobb. "We have more than 500 offenders here and almost 400 staff members. It's like being in a little city."
Cobb isn't a big fan of prison dramas like Oz or Prison Break. "When I come home at the end of a day, that's not what I want to watch." When asked if those shows portray prison life realistically, Cobb replies, "the day-to-day lives of inmates are much more mundane. Life is usually pretty boring for them."
Archambault is a medium-security facility. "We have every type of offender here," says Cobb, "everything from two-year sentences to life sentences." One of the prison's most notorious inmates is Valeri Fabrikant, who murdered four of his colleagues at Concordia in 1992.
"When offenders come into the system, they're assessed for their risk level," Cobb explains. "That involves their capacity to follow rules, the risk they might pose to public safety and the risk of an escape attempt." Archambault's inmates "pose a medium level of risk.
"I'm responsible for the safety of everyone who works here and for the community that surrounds us. I don't ever take that lightly," says Cobb. She takes other aspects of her work very seriously too.
"A big part of our job here is to give [inmates] the tools they need to get their lives back on track," says Cobb. "I really believe in that as a fundamental value." Successful rehabilitation benefits everyone, she adds. "Almost all the inmates here get out of jail eventually."
Archambault prisoners have access to job training, education programs and counselling. "We try to help them rebuild relationships with their family members," says Cobb. Noting that many offenders arrive with addiction issues or other medical concerns, she adds, "We give them the opportunity to get their health back.
"In the end, it's up to the individual," says Cobb. "It's up to them to use [the resources available] or not to use them."
Cobb hopes they do. "There are so many factors in a lot of these guys' lives that contributed [to why they're here] -- sexual abuse, drugs, gang issues. Many of them started life off on the rough side. They didn't have the advantages that I enjoyed."
Cobb is careful to keep constant tabs on the environment inside Archambault. "When the climate [in a penitentiary] is crappy, there's a lot of tension. There's more fighting." She regularly meets with an inmate committee ("You get useful feedback") and receives daily briefings from her staff on what transpired during the previous 24 hours. "Walkthroughs are invaluable," Cobb says. "Just walking around, talking with staff and inmates. I try to do that at least a couple of times a week."
The warden in Cool Hand Luke famously lamented, "What we have here is a failure to communicate." That's a problem that Cobb is determined to avoid.


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Two of the most common issues to arise at costs management hearings are the extent to which the court can and should consider hourly rates, and whether contingencies are justified, necessary or useful. It is now commonplace for judges to refuse to consider the issue of hourly rates, often with the refrain: "This is not supposed to be a detailed assessment!" The approach to budgeted contingencies varies significantly between courts. Both issues fell to be considered in the High Court by Warby J in the case of Tim Yeo MP v Times Newspapers Limited (2015). Advocate Nick Thornsby looks at the guidance provided and how it relates to his own experience of costs management
Read the full article on our dedicated website for insurance professionals >>This information is intended as a general discussion surrounding the topics covered and is for guidance purposes only. It does not constitute legal advice and should not be regarded as a substitute for taking legal advice. DWF is not responsible for any activity undertaken based on this information.


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We are a leading supplier of high quality innovative IT solutions. We understand the needs of the public sector and have a suite of solutions which can really make life easier and improve service delivery. We are a certified partner of many world-wide leading producers of hardware and software. We offer a number of proprietary solutions which integrate with them and make them easier to use. Our IT engineers are highly experienced in complex projects and are trained to the highest professional standards. We understand your challenges, design a bespoke solutions for you and supervise the optimisation of business processes and make sure it works and delivers the besr results for you. Our main areas of implementation expertise include: planning, purchase and logistics, sales and marketing, data analytics and visualisation, reconciliation and audit.
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About the Book
Features & Details
- Category Arts & Photography
Standard Portrait, 8×10 in, 20×25 cm
100 Pages
- Publish Date Oct 26, 2008
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About the Creator
Christine Walsh-Newton, Designer
Dover, Ohio
A BlurbNation book designer specializing in photo books. Your wedding, family reunion, vacation or portfolio quickly and affordably designed to your specifications. 115 Blurb books designed to date. Text books, poetry books and other book types welcome.
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William Tooker
William Tooker (or Tucker) (Exeter, 1557 or 1558 – Salisbury, 19 March 1621) was an English churchman and theological writer.
Born at Exeter in 1557 or 1558, he was the third son of William Tooker of that town by his wife Honora, daughter of James Erisey of Erisey in Cornwall. He was admitted to Winchester College in 1572, and became a scholar at New College, Oxford, in 1575, graduating B.A. on 16 Oct. 1579 and M.A. on 1 June 1583, and proceeding B.D. and D.D. on 4 July 1594. In 1577 he was elected to a perpetual fellowship, and in 1580 was appointed a canon of Exeter. In 1584 he was presented to the rectory of Kilkhampton in Cornwall, and in the following year resigned his fellowship on being collated archdeacon of Barnstaple on 24 April.
In 1588 he was appointed chaplain to Elizabeth I and rector of West Dean in Wiltshire. In 1590 he became rector of Clovelly in Devon, but resigned the charge in 1601. On 16 February 1605 he was installed dean of Lichfield, resigning his archdeaconry. According to Thomas Fuller, James I intended the bishopric of Gloucester for him, and actually issued the congé d'élire, but afterwards revoked it. Tooker died at Salisbury on 19 March 1620-1, and was buried in the cathedral. He left a son Robert, who in 1625 became rector of Vange in Essex.
Tooker was a good scholar, and, according to Fuller, 'the purity of his Latin pen procured his preferment.' He was also a skilful courtier in his choice of topics. In 1597 he published Charisma sive Donum Sanationis (London), a historical vindication of the power inherent in the English sovereign of curing the king's evil. This work won him especial regard from Elizabeth I, whose possession of the power was a proof of the validity of her succession. Tooker traced the healing power back to (the legendary) Lucius of Britain; but he rejected the contemporary beliefs about touch pieces as superstitions.[1]
In 1604 he published a treatise entitled Of the Fabrique of the Church and Churchmens Livings (London), dedicated to James I, whose chaplain he was, in which he attacked the tendency of puritanism towards ecclesiastical democracy, on the ground that it paved the way for spiritual anarchy. Besides the works mentioned, he was the author of Duellum sive Singulare Certamen cum Martino Becano Jesuita (London, 1611), written against Martin Becanus in the allegiance oath controversy, in defence of the ecclesiastical authority of the English king, to which Becanus replied in Duellum Martini Becani Societatis Jesu Theologi cum Gulielmo Tooker de Primatu Regis Angliae, Mainz 1612.
- ^ Marc Bloch, The Royal Touch: Monarchy and miracles in France and England, p. 23 and p. 182.
- This article incorporates text from a publication now in the public domain: "Tooker, William". Dictionary of National Biography. London: Smith, Elder & Co. 1885–1900.


text: Our old site was taken offline some time ago due to some problems with it. It had not been updated for a while and a new site was badly needed. The new one is based on the Prana Pro WordPress template. It's currently more or less an empty shell and the real content will be added as soon as possible.


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Take Action
- Tell the ASA They're Wrong HERE
- Should Porn Ads be in Newspapers? VOTE HERE
- Read our Press Release Here
In early 2016, we made a formal complaint to the ASA and asked them to decide if it was 'socially responsible marketing' for The Sport sold as a newspaper to carry ads for prostitution and pornography. It took them 6 months and a special Round Table meeting to decide that it was 'as long as the ads are not on the back cover as then (and apparently only then) children may see them'.
The entire content of The Sport newspaper is porn and sex ads alongside pornographic images of naked teenage girls. But the ASA was going to apply this ruling to ALL NEWSPAPERS.
However, the ASA has agreed to make a fresh ruling on porn and sex ads in genuine newspapers so we have lodged a compaint about the Evening Standard's sex industry ads and, 6 weeks on (!), we await their response.
'Child Protection Lies at the Heart of Our Work'
ASA Guide to Parents
What We Are Doing
- We have got the ASA to make a new ruling about real newspapers, not the pornographic Sport 'newspaper'. Read it Here
- We have met with the ASA's new Gender Project Team
- At the bequest of the ASA, we have submitted recommendations to improve their codes and their rulings. Read it Here
'Ads likely to be seen by children must not be harmful'
ASA Guide to Parents
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ASA Guide to Parents