text: Viewing That Funny Show - 4 of 5 Most Common Subject Headings A couple breaks up just before they win the lotto and the girl takes off back to... more »A couple breaks up just before they win the lotto and the girl takes off back to russia unknowingly. The wants to be fair and share it 50/50 since they bought it together... but she deletes all his email attempts because all his subject headings are the most common spam headings. By the time she opens one a month later, it was just to tell her he already spent it on outrageous things he did not need. « less Kelvin Ray November 17, 2010 310 Views More Info Full Credits Kelvin Ray Uploader Stats & Data 7Funny4Die 310 Views November 17, 2010 Published Category Web Series Sketch Clean Comedy Tags email technology spam subject headings headings spam email russia lotto lottery Colorado bangbros sky mall skymall computer Up Next: Shrooms Good AUTOPLAY Up Next In: 10 Shrooms Good Deadbeat Dad You can, my kids' in the trunk. PSA: Never Shake a Baby (with a twist ;-) Follow Browse Related Comments That Funny Show 5+ more View All ▶ 10+ more View All ▶ Kelvin Ray Vaguely Related Videos Vaguely Related Pics & Words Advertisement Tags: email technology spam subject headings headings Advertisement


text: For those of you that use enclosed trailers,what kind do you recomend? I have a 20 ft wells cargo that needs to be replaced. I went to the local dealer and was not very impressed with the prices or the salesman. I want to hear from you guys out there what enclosed trailers are great and which ones to stay away from.

subset: commoncrawl


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track 6 of science 1994 also reffered to as ''woolworm''
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| This article is a stub.
You can help the Walking Dead Wiki by expanding it.
Wylie Herman is a voice actor who potrayed Matthew in Telltale Games' The Walking Dead: Season Two.
- Voicing Matthew was Wylie's first professional act, though the episode was released after his first published work, "To Deborah".


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Beautiful Baby Contest
We are proud that the PRINCE GEORGE'S SOIL CONSERVATION DISTRICT is the sponsor of the Prince George's County Fair Beautiful Baby Contest!
Come enter your beautiful baby in the 2016 Prince George's County Fair Beautiful Baby Contest! Held at 6:00PM on Thursday, September 8, 2016. Contest is open to PRINCE GEORGE'S COUNTY RESIDENTS only.
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Boys and girls will be judged separately in each of the following categories (Twins will be judged only if there are enough entries in this category).
You may register when you arrive.
It is recommended that you print out the entry form ahead of time and have it filled out before you arrive.


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text: Hi I noticed that my N/S front pads have twice as much meat left on them compared to the O/S. The O/S looks as if they'll need replacing very soon. Is it common for them to wear unevenly? Or could I have a sticky calliper? I've never noticed the car pulling to one side under braking and the discs look to be worn evenly. A set of DS2500 are on my shopping list ... Cheers, Liam


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Marshawn Lynch didn't get the memo about not using his hands while playing soccer.
Or, more likely, he didn't care.
Playing in a charity soccer event near Seattle, the former Seahawks and now Oakland Raiders running back entered the game in the second half wearing official Beast Mode socks and flip-flops.
And that's not the only unconventional move made by the newly unretired NFL star. Upon receiving a pass, Lynch picked up the ball and ran it into the goal. Then, after he was given a red card, Lynch stole the card from the...
Seattle Seahawks quarterback Russell Wilson (3) throws a game-winning 24-yard touchdown pass on the final play against the Green Bay Packers at CenturyLink Field. The Seahawks defeated the Packers 14-12.


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Elsie Lapp, the manager of Halfway's country store, is a voice of cheer and goodwill for her customers. But Elsie's serene world spins out of control with the terrible crash, and the young woman finds herself grappling for the simple faith that has always sustained her. Her burden is eased by Ruben Zook, a neighbor known for his snide jokes. In the aftermath of the accident, Ruben reveals a tender heart that could easily sweep her away. However, Elsie carries secrets that keep her from surrendering her heart.
|Format: Paperback
Number of Pages: 370
Vendor: Ballantine Books
Publication Date: 2013
|Dimensions: 8 X 5.1 (inches)
Series: Lancaster Crossroads


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Passionate lover of beauty in all its forms, with a desire to touch the hearts of all I encounter and share the beauty of life with others
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Tag Archives: War poem
The Christmas Truce by Carol Ann Duffy
The subject matter has woven its way into our collective consciousness, but it has perhaps never been so beautifully described as in this poem by the current poet laureate (and the first woman to hold the post), Carol Ann Duffy. … Continue reading
Posted in Inspiration, Literature, Poetry, Spiritualism Tagged Carol Ann Duffy, Christmas, First World War, History, Inspiration, Literature, Moving, Peace, Poem, Poet laureate, Poetry, Spirit, The Christmas Truce, War poem Leave a comment


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Ottimo Cooking Academy
|Ottimmo Cooking Academy
Tempatnya menarik, dengan tampilan yang eye catching dan dapur yang lengkap dan mewah. Di sini saya bertemu dengan Pak Anton yang mempersilakan kami ke Bistro VII, resto yang satu lokasi dengan sekolah masaknya.
a while ago I had the opportunity to go to Ottimmo, a cooking academy in Citraland area, Western Surabaya. I got an invite as a jury on a cook-off between two groups of student.
The place is interesting, with eye catching design and lots of banner showing the photos of their students in front. The kitchen is nice and looks very professional.
Upon arrival we were greeted by Mr Anton, the counsel for the academy, which shows us around and then to their restaurant in the same area, Bistro VII.
|Ottimmo Cooking Academy - Library
Their library is quite fancy, with lots of interesting cook books written by celebrity chefs such as Gordon Ramsay, Nigella Lawson and Jamie Oliver. Being a fan of food and reading, I'd wish we spent more time in the library ^^.
|Ottimmo Cooking Academy- Bistro VII
Berikut beberapa hidangan yang kita coba di kesempatan saat itu. Berhubung cook off, para juri tidak diberi keterangan lengkap baik nama maupun deskripsi makanan, hanya hal-hal dasar seperti jenis ikan dan sebagian saus yang dipakai, supaya pendapatnya obyektif.
This are some of the dish that we had on the occasion. Since it's a cook off, we only had a brief description of the dish such as what type of fish or protein used, in order to for the judgement to be objective.
|Appetizer in a pot (Ottimmo Cooking Academy)
|Appetizer in a pot (Ottimmo Cooking Academy)
|Duck & Egg Crispy (Ottimmo Cooking Academy)
|Duck & Egg Crispy (Ottimmo Cooking Academy)
Main Course
|Hallibut and Shrimp (Ottimmo Cooking Academy)
|Hallibut and Shrim[ (Ottimmo Cooking Academy)
|Trio Main Course (Ottimmo Cooking Academy)
|Salmon (Ottimmo Cooking Academy)
|Salmon (Ottimmo Cooking Academy)
|Pistachio Cream (Ottimmo Cooking Academy)
|Pistachio Cream (Ottimmo Cooking Academy)
|Dry Ice for appearance
|Cocoa Shell (Ottimmo Surabaya)
|Cake inside of the Cocoa Shell (Ottimmo Surabaya)
Hidangannya di sini sangat menarik, berani menggunakan bahan yang aneh dan tatanan yang tidak konvensional. Berhubung yang masak adalah para murid, memang masih ada rasa yang kurang pas atau penggunaan bahan yang tidak bisa dimakan, tapi tetap sangat menarik, apalagi mengingat chef-nya masih belum lulus.
Rasanya tidak sabar untuk mencoba kreasi mereka setelah lulus, bakal semakin memperkaya ragam kuliner di Surabaya.
To say the dish was interesting is an understatement, the students were really creative, creating a unique and delicious dishes. Granted not all was a hit, some were tasty and some didn't taste great, keeping in mind they were still students, the creation were fantastic nonetheless.
Looking forward to their creations to further alleviate Surabaya dining scene when they all graduated.
Ottimmo International MasterGourmet AcademyJl Telaga Golf TC4 no 2-3, Citraland


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Home > Product Categories > Pro-Video > Tripods & Camera Support > Tripods & Fluid Heads > DOLLYPOD V
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A. Ruiz


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Scene Pictures
Watch more humiliating clothed male / naked male videos
At CMNM, Aiden can't believe how far the other workers at the garage are willing to go to humiliate him during his apprenticeship. They're absolutely determined to have this boy's virgin arse and after forcing him to sit on one of their faces, they bend him over and stick their fingers up his tight sphincter. Aiden is outraged, but is powerless to stop them.


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What Are the Best, Biggest Movies of the Last 20 Years?
February 27, 2017 - We take a look at how often he Oscars' Best Picture winners line up with the highest grossing and best reviewed movies of each year.
Oscar Bait Movies That TV Tackled First
November 15, 2013 - STAX'S BLOG: You know about Argo, The King's Speech, and 12 Years a Slave. But do you know of these earlier TV movies?
Are Oscar Contenders the New Blockbusters?
August 20, 2013 - Iron Man 3 aside, blockbusters have struggled this summer, while the success of last year's critical darlings suggest that tastes might be changing.
A King, a Moron, Cops and Liu Kang
April 19, 2011 - The Oscar-winning The King's Speech and the flop Gulliver's Travels arrive on Blu-ray, alongside Street Kings 2 and Mortal Kombat.
King's Speech Extended
March 9, 2011 - Anchor Bay has announced a few additional bonus features for Oscar winner The King's Speech on Blu-ray.
King's Speech Delivered
January 26, 2011 - One of the front-runners for several big awards at this year's Academy Award event has stepped out from the home-entertainment shadows via an early announcement to retailers to deliver a speech in your living room. The King's Speech has been cleared to go to home video from its distributor, and one of our favorites at that, Anchor Bay Entertainment. It's nice to see a small distributor with a good reputation delivering such a big title.


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|2008-02-05, 19:38
|Link #301
Did someone call a doctor
Join Date: Apr 2007
Age: 34
Killing people as a holy priest was worse, used to run around with PW:S, Renew, and Inner Fire up and club people to death with my stick, or mace + skeleton book. Damn it was funny, when people died to a melee priest. Its like a firestone warlock, except, worse.
Warriors are nuts if they get a roll on.
Paladins were a lot of fun to heal with back in the day when I played mine, just keep, going and going and going and going and going and going.
Consecrate doesn't break flag caps? I thought they changed that a while back. Not that I PvP much mind you, enhancement shaman aren't exactly the most pvp efficient class.
|2008-02-05, 20:00
|Link #302
Salt Levels Critical
Join Date: Oct 2007
I think Consecrate originally did break flag caps but they changed it because one pally spamming that with shield up and healing himself could hold a flag against an entire team for a significant amount of time. It definitely does not break it now though, I cap through it all the time. Hunter Volley also doesn't break it but I have no idea why on that one.
|2008-02-05, 21:01
|Link #303
An Intellectual Idiot
Join Date: Aug 2007
Location: The Internet, ranging from the World of Warcraft------Deviantart----and much more!..My mostly WoW
Age: 25
|2008-02-06, 05:49
|Link #304
Junior Member
Join Date: Feb 2008
enough already
ok, i've read much of this thread, though not all of it. I just want to say a couple things.
1. How many of you actually have lvl 70 character? it doesn't sound like alot, though some of you do know exactly what they are talking about. Reason being this. There are alot of stupid lvl 70's running around true, but they are not in the Guilds capable of downing said bosses. The amount of coordination and teamwork needed to navigate Mt. Hyjal, and Black Temple is borderline insane.
2. I think you all have to look at the story from a different perspective than the RTS warcraft games. This is sort of a look inside the world, not a continuation of those stories. I prefer to look at as a simultaneous though separate experience.
3. In terms of game play, nothing comes close to WoW. It has the best feel and control of any mmo, and imo is in a league all by itself. The amount of gear available is absolutely mind numbing compared to other games, and blizzard leaves it up to the player to sort the crap from the good. What is good for a warrior is not good for a paladin though they both wear mail/plate. A elemental shaman can wear wizard gear if he likes. just a couple examples.
4. The depth of character building. No other game comes close. With each class having 3 builds, and each build needing specific gear to work properly you can basically have 3 sets of gear for each character.
5. The economy. Though not good on all servers, the general idea is the best of any mmo. with 100 cp = 1 sp, and 100 sp = 1 gp. that makes 10,000 copper = 1 gold. the other mmo's with only one kind of currency get ridiculous. for example in Flyff you can easily pay 1million penya(sp) for some items. that would be 100 gold in warcraft terms. So when you pay 5000gp for your epic flying mount training, think how that would be in other games.
now as to not be completely off topic I will mention the next expansion.
lvl 80. Sounds great. Upgrading Nax to a lvl 80 dungeon is a wonderful idea. That way kel'thuzad will be back. Epic class Deathknight sounds great. Blizzard is already upgrading content throughout the entire game as well as making leveling from 20 to 60 much easier. A new continent to explore is a welcome addition. New content is always welcome. The grind from 60 to 70 wasn't bad with a bunch of 5 man dungeons along the way, and more quests available than you would ever need to get there. If the grind up to 80 is done in a similar manner there will be no complaints. Hopefully they make a lot of useful material for the crafting skills as they are currently barely usable. Other than making bags, and Leg enchants, and the occasional tailor set for casters the crafting skills are worthless. Enchanting is the most useful skill as it upgrades everybody and is the only means to shards. Hopefully they keep enchanting in check and don't go overboard.
The downside. All my lvl 70 epics are going to be worthless. Had to deal with that when BC came out and made all my lvl 60 epics worthless. (Except Anathima which i used till lvl 70 then promptly replaced.) Playing on PvP server will be annoying. A lvl 69 has no chance against a lvl 70, what about a lvl 80, he will have free reign on Azeroth populated by low level players. Think about it. 10 lvl 70's won't be able to kill one lvl 80. I'm expecting tanks with around 25k HP, and mage spells that do 7-8k dmg. to compare, a complete tier 2 tank from before BC had about 6k hp. PvE servers recommended for the feint of heart. The biggest downside, All Blizzard employees that play are definitely Horde Warlocks. They are so OP it isn't funny. I mean Blood Elf warlocks? WTF is that? So a new Horde warlock race wouldn't surprise me one bit.
Weighing everything that I know to be true, and a few things that have been whispered, I am looking forward to the expansion. It should make the game more enjoyable for those of us who love the end game experience, and add a bunch of content for those currently leveling and progressing through the game. But make no mistake, this game is all about End Game content and PvP. Currently the game doesn't even get started till you reach lvl 70, and that wont change after the expansion. And above all this is a game of skill, (unless you're a warlock, I played my brothers warlock in AV a few times, having never played one not even one minute, and after 5 minutes of getting used to the UI I was unstoppable. Really this is true) that requires teamwork, dedication to your fellow players, and sacrifice for the better of the guild. It cannot be played with selfish intent and expect to succeed. I hope you all can find something useful in this rant, and if not i'm sorry i couldn't provide the information you wanted. If anybody wants to know anything, and I have the information, I will be happy to help. good day to you all.
|2008-02-06, 06:57
|Link #305
Witch of Betrayal
Join Date: Jan 2006
I kinda have to agree with warlocks, though I'm biased since my main is a mage. But still, I just entered AB once with my friend's warlock never having played one and did far too well.
Though said warlock is pretty well geared with about 15000 health and 1000 shadow damage unbuffed.
|2008-02-06, 07:14
|Link #306
Junior Member
Join Date: Feb 2008
shadow preist
just a side note here on the shadow priest thing. I'm not taking this personally as I have always been a shadow priest. Shadow Priests are coveted for raiding as they have ambient healing to a group and ambient mana regeneration as well as pretty good overall dps. In a general 10min fight like Void Reaver I can literally recover thousands of points of mana and health to my group. however they must be speced for threat reduction and should have blessing of salvation, as well as reduced threat enchant to cloak to achieve maximum dps. They just cause so much threat. They are a strange class to play properly though as you need to monitor your cooldowns well, and know how much damage you can cause yourself in each situation.
On the PvP side of things, well they aren't too bad either. Nothing beats back to back crits with mind blast, and SWD. I can do almost 6k in a second and half when they crit back to back. and I only have 1000+ dmg and around 330 Resil in pvp gear currently. Not to mention a fear and a bubble to keep you alive, and the ability to own mages with silence and feedback. I can even take down warlocks a good percentage of the time. well about 35-40% is good for me against warlocks. so take that as you will. And anybody who says Warlocks are not OP is out of their mind. Especially Blood Elf Locks, they are absolutely sick.
Basically a shadow priest is not for everybody, and playing the class properly is as much an artform as a science. You have to beware of melee though, an MS warrior or a rogue will ruin your day in a heartbeat. I just like the class and to me its a lot of fun to be different and still useful. IE< Ret Paladins, Enhancement shamans, Fury Warriors are not so useful to a group.
Holy priests, IMO are overrated. yes they are very popular for pvp, and usually very formidable at that, but I'll take a holy paladin healing me any day. They can heal through all kinds of hell, take a beating and keep on going. And I will usually have no problem killing the opposing holy priest. I'm made to kill caster btw as a shadow priest. For raiding, all the healers are unique and bring their own benifits. Druids can innervate and battle rez as well as being the HOT characters, paladins have wonderful flash heals and DI, Shamans can chain heal and have wonderful heals overall, and priest have great big heals, and a few good unique heals, as well as fear ward. So they are all good at their own thing.
Anyway regardless of your spec, Priests rule. and i don't even want to get stared on the power of mass dispel. Many a paladin has met his fate after i remove his bubble and SWD him into oblivion. Dispel in general is very powerful. I just wanted to comment on how much I love my priest and all priest for that matter.
|2008-02-06, 11:33
|Link #307
Sleep beneath the flowers
Join Date: May 2007
Location: Lording above all of humanity >;3
Age: 27
It used to when I played since it ticks them for damage. I used it to detect Rogues trying to Sap me.
|2008-02-06, 11:44
|Link #308
Yandere for win :O
Join Date: Jun 2006
Location: Singapore
Not that people could roll their faces on the keyboard and win, but still...I'd hope for better (and tougher) things in the upcoming Sunwell to test myself (and my raid).
2) Blizzard has already layed down the hammer that WoW does indeed carry on the official story of Warcraft. Of course, if one would still choose to disregard this point as part of their "gaming immersion", all the power to them. ^_^
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~
Oh priests, jeez I hate them in arena xD. Mana burn single-handedly makes paladins cry with much sadness. Priests are rocking the 3v3 and 5v5s at the moment (at 2200+), being the most popular healing class pick (yes, even beating out druids in 3v3 and paladins in 5v5). For statistics :
Disc priests are just insanely good at the moment. Not that I'm calling / hinting for a nerf but I always cry when I see a Disc priest on the opposite side. xD
|2008-02-06, 11:50
|Link #309
Sleep beneath the flowers
Join Date: May 2007
Location: Lording above all of humanity >;3
Age: 27
In my experience just about any other class makes a Paladin cry because just about everything else can kill a Paladin pretty easily. >.>
I couldn't even easily kill Warriors under my level. They just totally outdamage me.
|2008-02-06, 12:17
|Link #310
An Intellectual Idiot
Join Date: Aug 2007
Location: The Internet, ranging from the World of Warcraft------Deviantart----and much more!..My mostly WoW
Age: 25
1. I have a 70
2. I'm not entirely sure what you mean
3. In terms of game play, nothing comes closes anymore....Star War Galaxies blue WoW away during it's first 3 years out...It was only when Lucas Arts and SOE changed the game to NGE when WoW really got it's star off. If it wasn't for the screw up that happend in SWG, WoW would be nothing...Ironically..if it wasn't for the SOE's screw up in Everquest, SWG wouldn't have been anything...See, when SOE screwd up Everquest, most went to SWG, and when SOE scewed up SWG, They went to WoW..I would know...I'm one of those people xD.
4. Again..SWG blew WoW away in terms of gear and talents...There were about 36 professions and you are able to choose 2, then you have to specialze in cetin branches to max out your character..plus trying to become a Jedi....ppfft..don't get me started
5. Now, WoW does have the best econmy I've seen.
I think the real problem with the grind from lvl 80 is the fact that there will be a huge Death-Knight Spree.....I mean, can you not imagine all of the people that will be on a Death Night by this time next year? I mean, I'm looking forward to the grind from 70-80..But the Death-Night...everyone will be on it and it will be rather annoying..
Oh yea...I completly agree...everyone spending all this time trying to get all this awsome gear just to have it worth nothing in a years time....
hehe..It's funny really..Everyone is all like HOE MAH GOSH KARA! AMEN! SSC! BT!!!!! by this time next year they'll be like...Who cares? All of them will become like Molten Core and Share in the lonlyness!!!!
|2008-02-06, 13:59
|Link #311
Sleep beneath the flowers
Join Date: May 2007
Location: Lording above all of humanity >;3
Age: 27
Ctrl-Alt-Del has just found the BEST way so describe my Arathi Basin experiences!
Now if you could only actually DO that... Life would be grand.
|2008-02-06, 17:41
|Link #312
Salt Levels Critical
Join Date: Oct 2007
|2008-02-06, 18:32
|Link #313
Kakashi Fangirl
Join Date: Jul 2007
Age: 33
i wasn't able to read all the pages, but can please someone tell me if the game is 3D? .... i wanted to get it, but when i read about the game it looks 3D or i was looking at those clips between the games ( please say its a normal game as the rest of World of Warcraft)
|2008-02-06, 19:00
|Link #314
Salt Levels Critical
Join Date: Oct 2007
|2008-02-06, 19:33
|Link #316
Sleep beneath the flowers
Join Date: May 2007
Location: Lording above all of humanity >;3
Age: 27
Unless there is just one guy. But now there is no why I can stop like say two or three people now since I don't attack that fast.
|2008-02-08, 12:29
|Link #317
An Intellectual Idiot
Join Date: Aug 2007
Location: The Internet, ranging from the World of Warcraft------Deviantart----and much more!..My mostly WoW
Age: 25
Man, on my server, Ali never wins bg....EVER! In the past week I've done about 60 bgs and didn't win a single one of them...On my server, You can't win unless it's a huge pre-made. Why? Because noone ever listens..EVER! And when few do listen, there isn't enough people listening ot come and help. In AV, mostly everyone goes off and finds Horde to kill....They don't realize that they need to go after the graveyards and stuffs...but they wont do it!!! It's annoying! Then another problem is the fact because the Horde always win, they get more honor allowing them to upgrade their gear quicker then Ali.
|2008-02-08, 13:44
|Link #318
Sleep beneath the flowers
Join Date: May 2007
Location: Lording above all of humanity >;3
Age: 27
Alliance usually always loses at BGs. I remember the time in AB when a pre-made from Dark Iron just steamrolled us in like... 2 minutes. I kid you not. They just slaughtered us and since no one wanted to defend they just capped all the flags.
|2008-02-08, 17:16
|Link #319
Salt Levels Critical
Join Date: Oct 2007
Too bad you guys aren't on the Cyclone battlegroup; despite what certain stats websites may say Alliance wins a significant amount of the bgs there, especially AV which has to be close to a 95% win rate. Never used to be that way but Horde has gotten a huge influx of idiots recently it seems. Not coincidentally most of them seem to be blood elves (I don't like to stereotype players based on what character race they have but some of this stuff really is true). It almost brings me to tears watching as we have 1 tower in EotS and have 4 blood elf hunters standing around in the middle, waiting for a flag that Alliance is holding instead of capping. And they are always named things like Pryncess or Cutiepie and have pets named Snuggles etc. All I can hope is that their stupidity one day causes them to click a keylogger and they remove themselves from the game.
|2008-02-09, 09:10
|Link #320
Witch of Betrayal
Join Date: Jan 2006
Situation is very different in every battlegroup. I play on EU Bloodlust, where Alliance in AV wins most of the time. In turn Horde completely dominates WSG. AB and EotS are pretty random, but Alliance have an advantage because half of the horde raid always joins late. (No idea why)
On the other hand I read the forums and there was a battlegroup where Alliance started boycotting AV (Horde 3h+ queue, gg) because "it's impossible to win"
|blizzard, game, mmorpg, online, warcraft, windows
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text:
High-throughput novel microsatellite marker of faba bean via next generation sequencing
Plant genomics
Faba bean (
A library with 125,559 putative SSR sequences was constructed and characterized for repeat type and length from a mixed genome of 247 spring and winter sown faba bean genotypes using 454 sequencing. A suit of 28,503 primer pair sequences were designed and 150 were randomly selected for validation. Of these, 94 produced reproducible amplicons that were polymorphic among 32 faba bean genotypes selected from diverse geographical locations. The number of alleles per locus ranged from 2 to 8, the expected heterozygocities ranged from 0.0000 to 1.0000, and the observed heterozygosities ranged from 0.0908 to 0.8410. The validation by UPGMA cluster analysis of 32 genotypes based on Nei's genetic distance, showed high quality and effectiveness of those novel SSR markers developed via next generation sequencing technology.
Large scale SSR marker development was successfully achieved using next generation sequencing of the
Faba bean (
However, faba bean suffers from several major biotic and abiotic factors that constrain productivity. Although significant genetic gain to overcome these has been made through traditional breeding practices
Recent advances in next generation sequencing (NGS) technologies enable the generation of large volumes of sequence efficiently and cost-effectively
A limited number of characterized SSR loci (<120) which have been validated over relatively few genetic backgrounds are available for faba bean. Initially, Pozarkova
Besides providing a cost-effective valuable source for molecular marker generation, the identification of SSR within ESTs is an effective approach for gene discovery and transcript pattern characterization, particularly if through mapping an EST-SSR or EST marker is significantly associated with a QTL
The present study identified high-quality putative SSR loci and flanking primer sequences cheaply and efficiently using the Roche 454 GS FLX Titanium platform. The resultant SSR sequences were characterized and validated through successful amplification of randomly selected target loci across a selection of faba bean genotypes from diverse geographic origin.
Plant material
A total of 247 faba bean accessions were selected from the National Genebank of China held at the Institute of Crop Science (ICS), Chinese Academy of Agricultural Sciences (CAAS), Beijing. Of these, 100 originated from China, 54 were from other Asian countries, 39 were from Europe, 30 were from Africa, 14 were from the America, 9 breeding lines were sourced from the ICARDA (International Center for Agricultural Research in the Dry Areas) faba bean breeding program and one was from Oceania (Additional file
Additional file 1
Table S1. The information of
Click here for file
DNA isolation, library preparation and 454 sequencing
Seven days after seed were left on moist filter paper in the dark at 22°C, sprouts from each of the 247 genotypes were collected. A single sprout of each genotype and of approximately the same weight was pooled and total gDNA was extracted using the CTAB method
Genome libraries were constructed using eight biotin labeled probes and a selective hybridization with streptavidin coated bead method
Subsequently, entire libraries were equally pooled and subjected to 454 sequencing with GS-FLX Titanium reagents at Beijing Autolab Biotechnology Co., Ltd (China). All processing and analyses of the sequencing data was performed with GS-FLX Software v2.0.01 (454 Life Sciences, Roche, Germany). Using a series of normalization, correction and quality-filtering algorithms, the 454 sequencing data were processed to screen and filter for weak signals and low-quality reads, and to trim the read ends for 454 adaptor sequences using the EMBOSS
SSR loci search and primer design
The software MISA (
SSR characterization and validation
The number of different types of SSR, length (motif bp × number of motifs) and SSR position was searched and analyzed for using a bespoke program written in MISA files
Marker assessment
Polymerase chain reactions (PCR) were performed in 20 μl reaction volumes containing 0.5 U of
The genotyping data was subsequently used to determine genetic relationships among 32
Quality inspection of the DNA library
The recombination rate within the constructed SSR-enriched
454 sequencing and characterization reads
A total of 578,251 reads were generated from the pooled library, and 532,599 read sequences were used for further analysis after adaptor removal. Adenine was the most abundant nucleotide (30%), followed by thymine (27%), guanine (22%) and cytosine (21%). The mean GC content was 43%. The average length of read sequence was 305 bp, with a maximum length of 635 bp (Figure
Figure 1
Frequencies length distribution of 454 read sequences
Frequencies length distribution of 454 read sequences.
Identification of SSR loci
After MISA analysis, the number of sequences containing an SSR was 125,559, and in total 250,393 SSR loci were detected. The number of sequences containing more than one SSR loci was 61,266 and the number of SSRs present in compound formation was 122,988 (Table
The total size of SSR motif sequences was 8,759,185 bp, with an average motif length of 69 bp. Of these, 25% comprised more than one discrete repeat and a high proportion (49%) was located within compound repeats. The majority of identified SSR motifs (83%) were located between the 5'-terminus and mid regions of the cloned sequences, and within 200 bp of the 5'-terminus (Figure
Additional file 2
Table S2. The primer pairs were successfully designed by Primer3.
Click here for file
Figure 2
The frequency of the SSR motif start position from the 5' terminus of the cloned insert within the enriched libraries
The frequency of the SSR motif start position from the 5' terminus of the cloned insert within the enriched libraries .
Abundance and length frequencies of SSR repeat motifs
The most common SSR motifs comprised trinucleotide and dinucleotide repeats (Figure
Additional file 3
Figure S1. Frequences of different SSR repeat motif types in mononuceotide.
Click here for file
Additional file 4
Figure S2. Frequences of different SSR repeat motif types in dinuceotide.
Click here for file
Additional file 5
Frequences of different SSR repeat motif types in trinuceotide.
Click here for file
Additional file 6
Figure S4. Frequences of different SSR repeat motif types in tetranuceotide.
Click here for file
Additional file 7
Figure S5. Figure S3. Frequences of different SSR repeat motif types in pentanuceotide.
Click here for file
Additional file 8
Figure S6. Frequences of different SSR repeat motif types in heaxanuceotide.
Click here for file
Figure 3
Frequencies of different nucleotide repeat sizes within the clones analysed
Frequencies of different nucleotide repeat sizes within the clones analysed .
Compound SSR analysis
Two types of compound SSR were identified; those without an interruption between two motifs (ie (CA)12(ACG)37 and noted as C* type) and those with an interruption between two motifs ( ie (AAC)7gtcaat(AAC)5 and noted as C type). In total, 1,893 C* type and 59,369 C type compound SSR loci were detected among those sequenced, reflecting the complexity of the faba bean genome.
Validation of SSR assay
Of the 150 primer pairs selected for validation of SSR locus amplification, 102 produced a reproducible and clear amplicon of the expected size. Of these, 94 (63%) were polymorphic among thirty-two genotypes assessed (Table
The dendrogram showed that the 32 faba bean genotypes fell into four distinct clusters (Figure
Figure 4
UPGMA dendrogram of 32 genotypes of faba bean
UPGMA dendrogram of 32 genotypes of faba bean .
This study demonstrated that massively parallel sequencing technology offers opportunity to quickly identify large numbers of high quality SSR with diverse motifs from a genetically orphaned species such as
The conversion of SSR-containing sequences into single locus markers may have a low success rate due to complex and/or insufficient flanking sequence. For example, just 20% of the identified dinucleotide repeats from spruce were converted to clear, discrete markers
Previously, a total of 304,680 reads were generated and 802 EST-SSR primer pairs were designed from transcriptome sequencing of faba bean
This work represents a major advance in the identification of large numbers of informative SSR loci in
SSR: Simple sequence repeat; QTL: Quantative Trait Locus; MAS: Marker-assisted selection; NGS: Next generation sequencing; EST: Express sequence tag; NCBI: National Center for Biotechnology Information; CTAB: Cetyltrimethylammonium bromid; MISA: Microsatellite identification;
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
TY performed bioinformatic analysis, primer design and drafted the manuscript. SYB created the SSR sequences rich DNA library, and participated in 454 sequencing. RF assisted in designing experiment and preparing the manuscript. TJJ tested SSR markers. JPG and YHH prepared all the seeds of
This work was supported by the National Natural Science Foundation of China (no. 31101198), the China Agriculture Research System (CARS-09) from the Ministry of Agriculture of China and Social Development of Science and Technology Plan (no. 2010BB007) from Yunnan Government. We are grateful to Dr. Dahai Wang and Liping Sun (Beijing Autolab Biotechnology Co., Ltd) for their special contribution to this work.
Model legumes contribute to faba bean breeding
Genetic variability for feeding value of faba bean seeds (Vicia faba): Comparative chemical composition of isogenics involving zero-tannin and zero-vicine genes
Reference standards for determination of DNA content of plant nuclei
Applications of next-generation sequencing technologies in functional genomics
The impact of next-generation sequencing technology on genetics
Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection
Genome-wide association studies of 14 agronomic traits in rice landraces
Simple sequences are ubiquitous repetitive components of eukaryotic genomes
The abundance of various polymorphic microsatellite motifs differs between plants and vertebrates
Genetic diagnostics in plant breeding: RAPDs, microsatellites and machines
Development and characterization of microsatellite markers from chromosome 1-specific DNA libraries of Vicia faba
Simple sequence repeats (SSRs) in faba bean: new loci from Orobanche-resistant cultivar 'Giza 402'
Generation and characterization of 11 novel EST derived microsatellites from Vicia faba (Fabaceae)
Development and characterization of 21 EST-derived microsatellite markers in Vicia faba (fava bean)
QTL mapping of ten agronomic traits on the soybean (Glycine max L. Merr.) genetic map and their association with EST markers
Incorporation of sequenced cDNA and genomic markers into the soybean genetic map
Characterization of the soybean genome using EST-derived microsatellite markers
A plant DNA minipreparation: Version II
A rapid total DNA preparation procedure for fresh plant tissue
Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers
Isolation of human simple repeat loci by hybridization selection
Isolating microsatellite DNA loci
EMBOSS: the European molecular biology open software suite
Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.)
Population genetic analysis of co-dominant and dominant markers and quantitative traits
Estimation of average heterozygosity and genetic distance from a small number of individuals
MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0
Molecular variation among Chinese and global winter faba bean germplasm
Genetic diversity and relationship of global faba bean (Vicia faba L.) germplasm revealed by ISSR markers
Microsatellites for linkage analysis of genetic traits
Automated sizing of fluorescent-labeled simple sequence repeat (SSR) markers to assay genetic variation in soybean
DNA typing and genetic mapping with trimeric and tetrameric tandem repeats
Survey of trinucleotide repeats in the human genome: assessment of their utility as genetic markers
A collection of tri- and tetranucleotide repeat markers used to generate high quality, high resolution human genome-wide linkage maps
Genome-wide distribution and organization of microsatellites in plants: an insight into marker development in Brachypodium
Identification and characterization of microsatellites in Norway spruce (Picea abies K.)
Occurrence and inheritance of microsatellites in Pinus radiata
Abundance, variability and chromosomal location of microsatellites in wheat
Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers
Analysis of a diverse global Pisum sp. collection and comparison to a Chinese local P. sativum collection with microsatellite markers


text:
The Dennar IV Suppression was an Imperial military campaign carried out by the Howling Griffons Space Marine Chapter against Chaos Cultists on the Agri-World of Dennar IV in 109.M40. During their long search for the Word Bearers Chaos Space Marine warband of Deklamus the Vaunted, the Howling Griffons 3rd Company under Captain Penvath Joachim responded to a planetary distress call from the world of Dennar IV in the Sangramentia Sector. This important supply world had succumbed to rot from within as the worship of the Ruinous Powers took root amid its flenser-cults and cult-clans. As the Howling Griffons descended on Dennar IV, they found only a handful of its city-states holding out against the horde of Chaos Cultists and daemon-possessed madmen, their walls packed with refugees fleeing the tide of mass-murder that had engulfed the planet.
Vastly outnumbered, the Howling Griffons deployed to the savannah, and wielding their superiority in armour and air power to stay mobile and spearhead assaults against the Heretics, smashed the cult forces in great scything sweeps before driving onwards to relieve the city sieges. The Loyalist inhabitants of Dennar greeted the Howling Griffons as divinely-sent saviours, and gladly rallied to aid them in liberating their world from the dark forces that assailed it, selling their lives alongside the Space Marines. The remaining campaign was bloody and ruthless, ever-afterward casting a nightmarish pall over the memory of Dennar's people. Since this victory, the Howling Griffons have maintained a lasting oath to protect the planet and the memory of the many martyred there for their loyalty to the Emperor, while the people of Dennar IV have kept faith with their saviours in providing them with victuals and the pick of their youth as Chapter recruits.
- Imperial Armour Volume Nine - The Badab War - Part One, p. 126


text:
Print advertisment created by Ageisobar, Brazil for Red Cross, within the category: Public Interest, NGO.
Creative Director: Carlos Domingos
Art Directors: Henrique Mattos, Cristiano Rodrigues
Copywriter: Daguito Rodrigues
Photographer: Sergio Buss
Highest Rated
If red cross gets it there shouldnt the guy in the truck be the donor then?
He should really move closer, otherwise the food (remedies?) would fall to the floor and that would be a real mess for everyone...
It looks dead. It smells dead. Yet it's moving around... That's interesting.
If red cross gets it there shouldnt the guy in the truck be the donor then?
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text:
If you'll believe in me, I'll believe in you
To see work scroll to the right
'Well, now that we have seen each other,' the Unicorn said to Alice, 'if you'll believe in me, I'll believe in you'
It's that simply ineffable space between the physical world of the day to day and that of unique memory, the transitional zone between conscious and unconscious psychic process, that continues to possess an inexorable dynamism and genesis of inspiration for Sydney based glass artist Lee Mathers. In what will be her first solo show for NG Art Gallery, If You'll Believe in Me, I'll Believe in You, Mather's unites elements of Scottish folklore, layers of latent memory and a subtle reference to Lewis Carroll's 'Through the Looking Glass' in an artistic exploration of perception and consciousness as a intermediary space between past, present and future and in turn articulates 'what you see' as really dependent on 'how you see.'
Initially, the work created for this exhibition began to manifest while Mather's was on a return trip to Lybster, a sleepy village on the east coast of Scotland. The rich cultural histories embedded in the town's dramatic coastal location, coupled with a seemingly endless light display of mirror-like hues flooding the landscape from sunrise to sunset, combined to form a circumstance in which "the legends of Scottish folklore, particularly those relating to magical beasts were completely believable." What would have been, given any other circumstance, an impromptu afternoon with 3 white highland ponies, instead became, in that place, at that time, a chance encounter with a little piece of Unicorn legend and for Mather's, a locus of the threshold between her physical and psychical worlds of experience.
Through a combination of glass as a sculptural medium, fabric, projection, and a perpetual fascination with the "interplay between light and shadow" the collections of work for this exhibition articulate Mather's faith in the ability to believe and the psychological processes involved in pursuing those beliefs. "For me" Says Mather's, "this creative exploration began with unicorns in the Scottish highlands and has somehow ended with origami Cranes in Sydney."
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text:
Fallout 3
Review by reckeweg
"Not since the days of Mad Max has an apocalyptic wasteland been so much fun"
Fallout 3 is one of the most anticipated titles of the year, and rightly so. This game is from Bethesda the makers of Elder Scroll IV: Oblivion. With such a predecessor expectations were high am I'm happy to say have been met in glorious fashion. You're going to read a lot of reports that tell you this game is simply Oblivion with guns and that is pretty much the case. However you need to decide if that is good or bad.
The game takes place in the year 2477 after the great war and you are a survivor from one of the great vaults courtesy of the Vault-Tec corporation. You'll go through a tutorial of sorts in the vault and once you bid your childhood home goodbye you'll emerge into world seemingly devoid of life. Nothing could be further from the truth for the desolate wasteland is home to a number nightmarish creatures.
Like any RPG there is plenty to see and do. You have a main quest that your character must complete in order to finish the game, but there are also a number of side quests that you can do and how you proceed is your choice. As you encounter various people and situations you will be faced with moral dilemma. Do you choose the high road and and exhibit the kind of actions that show humanity is worth a second chance or do you succumb to temptations of the flesh and show everyone that the world now matches humanities true nature?
Graphically the game is beautiful, it is visually a step above Oblivion. The control scheme is very easy to use and responds very well to your commands. There are a couple blemishes on the game but they are so minor that it really doesn't take anything away from the game. When playing in third person mode your character seems to glide across the ground rather than walk, but since this game is really meant to be played in 1st person it is almost a non-issue. There is also a minor glitch where some of the NPC's actually die from being dropped into the game. I know that sounds weird but it could potentially cause some real problems if it happened to someone important at an in opportune time. I'm really hoping Bethesda patches this in the future.
If you you have an adventurous spirit and love a game that will suck you in to its world for days at a time Fallout 3 is what you are looking for. With so many interesting characters, weapons and quests its sure to have you wandering the wasteland for a long long time. I highly recommend this game for anyone who likes games, it's definitely one of this years must haves.
Reviewer's Rating: 4.5 - Outstanding
Originally Posted: 11/03/08
Game Release: Fallout 3 (US, 10/28/08)
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Submit a review and let your voice be heard.


text: To get from Terni to Vigevano by train you can catch one of the 1 trains that everyday travel between these two cities. The first train to Vigevano leaves from Terni at 22:04 and the last train departs at 22:04. If you choose a local train the trip lasts on average 09:46 hours. For your trip from Terni to Vigevano by train you can choose to leave from one of Terni main stations: Terni On Wanderio you can compare all schedules, fares and railway companies to Vigevano and book the best itinerary starting from Terni.


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Flintville TN Real Estate & Homes for Sale
3 Properties Found
The median home value in Flintville, TN is $91,900.
This is
lower than
the county median home value of $98,300.
The national median home value is $185,800.
The average price of homes sold in Flintville, TN is $91,900.
Approximately 89.47% of Flintville homes are owned,
compared to 10.53% rented, while
0% are vacant.
Flintville real estate listings include condos, townhomes, and single family homes for sale.
Commercial properties are also available.
If you see a property you're interested in, contact a Flintville real estate agent to arrange a tour today!
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Courtesy: Leading Edge RE Grp Fayetville
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1300 sq ft home w/ new windows, flooring, paint, insulation & updated bathroom. Big eat in kitchen and the property has its own propane tank, storm shelter, carport that provides extra parking, & a new fill line.
Courtesy: Leading Edge RE Grp Fayetville
View additional info
COUNTRY ESTATE: 6+/- Acres & pond. Custom built home constructed with utmost thought given to plans & design. 3 master suites, 2 additional bedrooms, Large living room & dining area. Huge kitchen with island and all appliances remain! Custom cabinets, granite counters. Downstairs there is a built-in bar in the huge rec room, safe room, exercise room/office, 1 bedroom, bath and two car garage. (Manufactured home on 1 acre optional)
14 George Road Flintville, TN
Courtesy: BMA PROPERTIES
View additional info
Beautiful home sitting on 10+/- acres. Fenced for livestock. Office has closet and could be 4th BR. MA BR secluded downstairs with suite. Large eat-in kitchen and vaulted living RM and fireplace. Property needs TLC. Well needs filter system. Selling AS-IS
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What you can do!
You can create a video
Record music
Make your own website
Design pictures
Create pictures & much more!!! What is Multimedia? By:LurdesVasquez Multimedia is a combination of different variety's of medias like music ,video ,recording and much more. Formats Mp3 is for music and Mp4 video and music each are defined by a media Like swf. , .wmv, .mp3 and .mp4 Software Software is based on different programs like Microsoft or Power point, excel and much more.... You can create images that are 3D ,2D or 4D
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The Dom With the Clever Tongue by Sorcha Black, Leia Shaw, and Cari Silverwood
Story Rating: 5 out of 5 paddles
Sting Factor (kink): 5 out of 5
Reece and Scarlet have everything a loving couple could want, except a hot kinky sex life. Malachi, an experienced Dom, steps in to help them sort that out. Not only is he a terrible memory from Reece's past, he's a smartass and damaged goods too.
As they all learn to trust each other, Malachi becomes more than just their kink coach. It's a full-on brat smackdown, and no heart gets out unscathed.
Warning: Contains unrepentant brats, surprise orgasms, a Domme with training wheels, and a Dom whose tongue is registered as a weapon of mass seduction
When Black, Shaw & Silverwood write a book, you know it's going to be a good one! The Badass Brats Series is probably one of the better written series, by multiple authors, that anyone who enjoys erotica could read!
The relationship between Reece and Scarlet is something that a lot of people aspire to. Their openness with each other, their faults, likes and dislikes, and then you add kink into the relationship and they get just a bit lost. Enter the goofy Malachi. Ah, but there is past history between Reece and Malachi, he has to get her past that to see him as the man he is today, especially when he starts falling for her.
Then Malachi starts falling for Scarlet too, now what's he going to do? Luckily for them they get that figured out for the most part. Reese realizes she really and truly is a sub, but a brat at times too. This is part of the Badass Brats series for a reason! You will never view a clothespin the same ever again!
The entire story will keep you on the edge of your seat, or bed, or whatever! One of the best scenes in the entire story has to be Scarlet and Malachi and who is stronger. You knew that this was building up to a smackdown and you know who's going to win, but still, it is so hot that you're glued to the book and won't want to put it down!
Once again another spectacular read from the trio! I can honestly say that I love the Badass Brats series and can't wait for the next one because I know that I'm going to enjoy it just as much as I have the rest of the books in this series! You gals rock!
Reviewed by Cat


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text:
Specializing in model and radio controlled aircrafts for over 40 years, Norburn Model Aircraft Supply provides enthusiasts with the widest and best selection in British Columbia. Creating a unique business card, we printed and laser die cut balsa wood a small glider that is functional once assembled. You can watch the video of assembly.
Direct advertisment created by Rethink, Canada for Norburn, within the category: Retail Services.
Creative Director: Ian Grais, Chris Staples
Art Director: Jeff Harrison
Producer: Sheila Santa Barbara
Highest Rated
Idea and execution both fly.
Write a wise saying and your name will live forever – Anonymous.
One more creative idea for your business card collections, Ivan http://www.moinid.com/2009/09/thermo-sensitive-business-cards
the black nose comes maybe only the premium business card. the normal business partners don't get them. ;)
i wish i could work at an agency like rethink, and come up with random ideas all the time that the agency was willing to use agency money to produce it, and just give it to a client so they could enter it into award shows.
No kidding. It's a lame waste of time. I came up with this idea three years ago. Along with every other cliche for a business card using a material related to what they sell. It's obvious that stuff wins awards, not hard work. The biggest lesson you can get from this is, A, are you doing ads for the money, or B are you a hard worker and do real work because that's what matters, C are you passionately creative and do it because it's so obvious it just has to be done, D is your requirement for self appreciation so petty you need to be reassured daily by producing clearly obvious fake jobs for fake clients.
The answer is all of the above. People that do this are passionate hard workers that do obvious ideas to win awards and get more money and like all creative people are continually insecure and craving attention and acceptance from one project to the next. You either love it or you hate it, but based on this place one thing you can't deny is it's a cruel business.
Idea and execution both fly.
Write a wise saying and your name will live forever – Anonymous.
Cool idea
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text:
Regal is in this season, as are gilt, coffered ceilings and South American chic
When Oscar Niemeyer, the legendary architect and designer, died at the age of 104 in December, his passing from the Brazilian design scene was merely temporal, as both his work and legacy continue to infuse a sensuous, form-centric culture that the world is embracing with increasing interest and ardour. From Niemeyer and his contemporaries to younger innovators like the flamboyant Campana brothers, Brazil's many design mavericks are no doubt getting a boost from the global attention the country is attracting as host of two upcoming global extravaganzas: the World Cup in 2014 and the Olympics in 2016. This past week, Canadians got the opportunity to have a rare close-up look at classic Brazilian designs when the upscale Toronto retailer Avenue Road launched 4 U From Brazil, a promotion of the work of four Brazilian giants: Niemeyer, Paulo Werneck, Jorge Zalszupin and Carlos Motta. Motta, who travelled north for the launch, is making his Canadian debut: His furniture designs are being shown for the first time in this country exclusively at Avenue Road. The retailer will also carry the manufacturer Etel's reissue of select Niemeyer pieces, including his marvelous Rio chaise. Rio chaise by Oscar Niemeyer, $32,125, at Avenue Road (www.avenue-road.com).
What are the emblems of our digital age? There's the Apple logo, of course. And Bill Gates's bowl cut. Perhaps the single most symbolic, however, is the pixel, a computer-era icon that has appeared on fashion runways periodically, but hasn't been adopted so enthusiastically by the world of interiors. Until now. This season, outfits as varied as high-end tile makers and big-box retailers have gone dotty for the motif. Why? At a time of political and social fragmentation, a distinct element that joins together with other distinct elements to create a vibrant yet cohesive whole has obvious emotional and aesthetic appeal. And least that's how I see it – all this could only be a test. Lacquered Italian jewellery boxes, $199.99 to $299.99 at HomeSense (www.homesense.com).
(Margaret Mulligan for The Globe and Mail)
Looked up lately? Recessed ceilings have been conspicuous design features of the suites and public areas in some of Toronto's finest new hotels, including the Shangri-La and the Trump. And when Stumptown Coffee Roasters undertook its most ambitious coffee-bar project to date, the $1-million transformation of a rundown Greenwich Village storefront, it incorporated, The New York Times reported in May, some "unusually sumptuous details," including a coffered ceiling. Long associated with baronial heaps, Loire Valley châteaux and the Pantheon in Rome, coffered or recessed ceilings have suddenly become more mainstream. And why not? They're not expensive to install, add character and the illusion of height, conceal or enhance lighting sources and provide a sense of permanence even in newly built homes. Just make sure, when installing such a ceiling, that the look is seamless, keeping the framework the same colour or material as the walls. If a little colour is desired, do as Behr Paints did in the staged setting pictured here, filling in only the ceiling's recesses (in this case with a rosy pink). Minuet Rose T14-15 (in recesses), Grand Soirée T14-13 (on framework and walls) and Cloisonné Blue T14-14 (on wall panels) are among Behr's new colours for 2014; visit www.behr.com for retailers.
The popularity of golden-toned decor ebbs and flows; this season's turn toward the regal (see below) means we're currently in a high-tide period. To create a pleasing and interesting effect, introduce gilt accent pieces of varying textures and materials to a room scheme, but keep the brightness factor at the same level, be it matte or luminous. And don't overdo it in terms of quantity: You want a touch of warmth and richness, not Caesars Palace. Gold pairs well with dark woods, natural stone such as marble and colours including black, brown, dark purple and many blues. Gold baskets, $25.90 and $39.90 at Zara Home (www.zarahome.com).
(Markus Lista)
Benches are so invariably handy, offering a perch for pulling your boots on or off in foyers, accommodating extra diners at long tables, simultaneously serving as settee, ottoman and side table in living rooms and boudoirs. Now, they are finally getting the haute treatment they deserve, becoming fall's must-have piece of furniture. Toronto's studio b, which unveils its luxuriously remodelled showroom today, has some of the season's choicest specimens, including this handsome Holly Hunt model (HH is new this fall at sb, the U.S. brand's exclusive distributor in Canada). Dragonfly bench by Holly Hunt, from $4,340 at studio b (www.studiobhome.com).
When Hermès releases a version, you know that the bench has reached new heights: Its sporty Cheval-d'Arçons bench, inspired by the form of a pommel horse, boasts a walnut body, bullcalf-leather upholstery and ingeniously concealed drawers in the middle and at each end of its long, curved frame. It also costs as much as some small cars – yet another benchmark! Cheval-d'Arçons bench, by special order at Hermès (www.hermes.com).
The search for an antidote to "stainless fatigue" continues apace. Sure, we've seen a number of alternatives to the stainless-steel appliances that have dominated North American kitchens hit the market, try to find favour and mostly become niche hues. (Hello, Oiled Bronze.) GE, however, thinks it has a winner: slate. "It's a warm matte grey, low-gloss, but with a slighty metallic finish," Lou Lenzi, GE Appliances' director of industrial design, said in a recent interview of the new appliance colour his company unveiled earlier this year to a limited consumer audience (and much reported enthusiasm) and will be rolling out across the U.S. and Canada this fall. According to Lenzi, slate works harmoniously with most cabinet and countertop materials and even partners well with existing stainless-steel appliances. "We appreciate that most people don't replace their entire [kitchen] suites at once," he said. So is slate likely to have legs – or will it show cracks? Here's what makes it a solid contender: It's a stylish neutral that won't date easily, it's flexible enough to appeal to wide range of tastes and, perhaps best of all in the context of (so-called) stainless, it's fingerproof-resistant. GE Over the Range Microwave Oven in Slate, $599 at GE Design Centres and Home Depot (visit www.geappliances.com for locations).
If a person's home is his or her castle, this is the season to explore that maxim literally. Starting with the Queen's Diamond Jubilee last year and culminating in the much-anticipated birth of wee Prince George in July (not to mention all those European weddings, abdications and coronations), monarchical comings and goings have been front and centre of late, taking on the feel of one big reality show (The Royal World?). This extended glimpse into regal doings clearly influenced the queenly couture at recent high-fashion shows in Paris. And now it's moving – slowly and elegantly – into the realm of housewares and furnishings. I am not, of course, talking about mugs bearing Camilla's mug, but the trappings of real royal life: chandeliers dripping with crystals, Louis-anything loveseats, lots of velvet and silk. And haven't you always wanted a toss pillow depicting a bewigged swell? Bordeaux loveseat, from $5,800 at Barrymore (www.barrymorefurniture.com).
Since "overblown," "overstuffed" and "over the top" are among this season's decorative catchwords, it stands to reason that black and white – the most graphic and eternal of all colour combos – should have its place. If you're bold, take a cue from renowned society decorator Dorothy Draper and pair black and white stripes with vibrant greens and reds or subtler pastels. The fainter of heart can ease into the look with a charming checkerboard floor or pair of striped lampshades against the beige backgrounds popular today. Whatever you do, avoid zebra skin – animal prints are so last year. This autumn, it's all about heightened artificiality, whether you're outfitting your castle or your condo, a palace or a pied-à-terre. Striped table lamp, $79.90 at Zara Home (www.zarahome.com).
(Markus Lista)


text:
help to setup vpn.
3 Replies
Jan 4, 2008 at 3:50 UTC
Setting up a VPN in theory is very simply, in practice it can be difficult. I suggest you start here: http:/
Let us know if you have questions
Jun 6, 2008 at 9:48 UTC
Hello, Vpn newbie here. I read the aticle that you posted, but it says I need 3 servers. We only have one server (Windows 2003 Server Std Edition) which is the DNS, DHCP and has file storage on it. We have a couple of saleman that need to connect in and view reports and sometimes create orders. They will need to have access to mapped drives. We have a Linksys router which I believe is setup correctly. I can connect to it from the client, but from there it is all fuzzy. Any help would be great! Thanks.
Jun 9, 2008 at 11:49 UTC
The implementation of this really depends on the kit you're using. The theory of it all is the same but putting it into practice can vary.
From your brief description I reckon you're probably best looking at PPTP tunnels into your VPN server for your sales staff. There is a PPTP client built into XP so you don't need any extra software here. Your staff can make the connection by opening up Network Connections and clicking on your VPN entry. There's a wizard for setting it up under "Create a new connection".
Your Linksys router documentation should explain how to set it up as a VPN server.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
|New Order
Nyū ōdā
|Chapter Info
|Release Date
|Aug 31th, 2012
|Volume 20
|Heart of Jupiter Arc
|Chapter Guide
A New Career in a New Flag
Ner Order is the 200th chapter of The World God Only Knows.
10 years ago, the night of July 19th. Keima has returned to the front of his old house again, the restart point, knowing this when he heard Elsie exclaim about his currently childish body. He was so frustrated by the fact that he has to go back to the ship again. But pondering on the name of the Mysterious Girl, which was stated to be "Dokurou", Keima noticed it was also the name of the chief Elsie served under. As a repetition of events, young Mari came, telling him about demons coming out at night. But for Keima, he was more concerned about the strikes in the orb. With currently two, he noticed before that there was four, then later three strikes in the orb. Judging from the events, he believed these strikes represented how many retries he had.
Tenri's mother apologized how she had left her husband starved. Mari told Keima to greet Tenri, which Tenri's mother asked the same to her own daughter. The two mothers pushed their child face-to-face. While Keima was calm, Tenri was all to shy to say anything. Though Keima was worried this new development would lead to a long one, Tenri instantly left, walking away as she popped the bubble wrap. Tenri's mother again apologized, this time for how her daughter was so shy, while Keima's mother did the same, saying her son was so unsociable. In the end, Keima's worries went to waste.Keima and his mother headed into the house. Even though Keima wanted to go to bed to quicken the conquest, his mother disagreed and forced him to take a bath with her. Even though he had experienced these kinds of loops in games, experiencing one first-hand was so annoying in comparison. By the time Mari had gone to sleep, Keima and Elsie hurried straight to the Akanemaru. Keima was this time infuriated by the fact that he had to do save a girl from falling, each and every time. When they were able to see the ship, the mysterious girl was already on the mast. When the girl jumped, before she hit the deck, Keima jumped and saved her in the nick of time. the first thing he told to the girl was his complaint of how he had to save her each time.
Keima called out the girl's name, Dokurou, clarifying that this was her name. He told her to take out the same orb he held in his hand, saying that the orb she has and the one he has is their bond. He told her that if she ever died, the same fate would come to him. The girl was silent, as Keima's head filled up with thoughts about restraining the girl from committing suicide, before dispelling the despair in her heart. The inquired as to how he knew about her, to which Keima replied that he just knew. Silence once again came, until the girl stated, as she has done before, that she didn't know. When she realized her surroundings, she was in the darkness, one that kept drawing her in.The girl suddenly started to regress to becoming younger and younger, which Keima wondered what he should do this time. Since her heart has an emptiness, he has to fill it up. Just as he has done in previous conquests, Keima gave a kiss to the age-regressing girl, who was currently just around the same childish-age as Keima was. He claimed that if she knew nothing of herself, then he would decide for her. He then commanded her not to die on her own accord, for he would do something about her despair. This triggered her regressing to stop, reverting her back to being her normal self. Nearby, Keima's orb shined, while the strikes within took upon a new pattern: two triangles, one inside the other. Did Keima manage to progress to the next level?
- There is an unnamed character in the cover who is beside Yui that will eventually appear in later chapters.
- ↑ Source: Red Hawk Scanlations


text:
Senior research fellow
email@example.com firstname.lastname@example.com
Research interest
Current research
Other activities
Gatekeeping and the utilization of physician services in France: Evidence on the Médecin traitant reform.
The Likely Effects of Employer-Mandated Complementary Health Insurance on Health Coverage in France
The Likely Effects of Employer-Mandated Complementary Health Insurance on Health Coverage in France.
How Will Employer-Mandated Complementary Health Insurance Impact Insurance Coverage in France?
Why is the 'healthy immigrant effect' different between European countries?
Explaining the Non-take-up of a French Health Insurance Vouchers Program (Aide à l'acquisition d'une complémentaire santé, ACS)
In What Way Can Primary Care Contribute to Reducing Health Inequalities?
Immigrants' Health Status and Use of Healthcare Services: A Review of French Research
Mediating role of education and lifestyles in the relationship between early-life conditions and health : evidence from the 1958 British cohort.
Giving up on Health Care for Economic Reasons: an Econometric Approach
Healthcare Renunciation: a Socio-anthropological Approach
Variations in preventive care utilisation in Europe.
Contribution of lifelong adverse experiences to social health inequalities: findings from a population survey in France.
Take-up Rate of a Subsidising Scheme for Acquiring a Complementary Health Insurance in France: Key Findings from a Social Experiment in Lille
Inequality of Opportunities in Health in Europe: Why So Much Difference Across Countries?
Lifestyles: a Channel of Intergenerational Transmission of Health Inequalities?
Effort or Circumstances: Does the Correlation Matter for Inequality of Opportunity in Health?
Monitoring Health Inequalities in France: A Short Tool for Routine Health Survey to Account for LifeLong Adverse Experiences
Inequality of Opportunities in Health in France: A first pass.
Immigrants' Access to Ambulatory Care in France
Social origins, early hardship and obesity: A strong association in women, but not in men?
Inequality of opportunities in health in France. Trannoy A., Tubeuf S., Jusot F., Devaux M., Health, Econometrics and Data Group (HEDG) Working Papers, n° 08/24, HEDG, c/o Department of Economics, University of York, 2008/10.
Can physician-patient interaction generate social health inequalities ? First results from a 2007 French pilot survey. Afrite A., Dourgnon P., Pascal J., Jusot F., Lang T., Lombrail P. and the Intermede project members, "16th Eupha Conference, I-health: Health and innovation in Europe. Lisbon, 6-8 November 2008", European Journal of Public Health, 2008, vol 18, suppl. 1, p. 117.
Access to psycho-social resources and health: exploratory findings from a survey of the French population. Jusot F., Grignon M., Dourgnon P., Health Economics Policy and Law, 2008/10, vol 3, n°4, 365-391.
Self assessed health of immigrants in France - Analysis of the 2002-03 decennial health survey
Alcohol consumption in France: one more glass of French paradox.
Impact of health care system on socieconomic inequalities in doctor use
Socioeconomic inequalities in health in 22 european countries, Johan P. Mackenbach, Irina Stirbu, Albert-Jan R. Roskam, Maartje M. Schaap, Gwenn Menvielle, Mall Leinsalu, Anton E. Kunst, The European Union Working Group on Socioeconomic Inequalities in Health, The New England Journal of Medicine, vol. 358, n°23, 2008/06/05, 2468-2481
Social heterogeneity in self-reported health status and measurement of inequalities in health
Job loss from poor health, smoking and obesity : a national prospective survey in France. Jusot F., Khlat M., Rochereau T., Sermet C., Journal of Epidemiology and Community Health, n°4, vol 62, 2008/04, 332-337.
Bad health strongly increases the risk of becoming unemployed or inactive
Psychosocial resources and social health inequalities in France: Exploratory findings from a general population survey. Jusot F., Grignon M., Dourgnon P.
Health working life expectancies at age 50 in Europe : a new indicator, Lièvre A., Jusot F., Barnay T., Sermet C., Brouard N., Robine J.M., Brieu M.A., Forette F., The Journal of Nutrition, Health & Aging, vol 11, n° 6, 2007
The shape of the relationship between mortality and income in France. Jusot F., Annales d'économie et de statistique, Health-Insurance-Equity, 2006/07-12, n° 83-84, 89-122.
Inequality of opportunities in health: the influence of parents' social status and health status
Unemployment and mortality in France, 1982-2002, Buchmueller T., Grignon M., Jusot F., Chepa working paper series n°07-04, Hamilton, ON: McMaster University, Centre for Health Economics and Policy Analysis (CHEPA), 2007/05, 32p.
Psychosocial resources and social health inequalities in France : Exploratory findings from a general population survey. Jusot F., Grignon M., Dourgnon P., Sedap Research Paper n°189, Hamilton, ON: McMaster University, Program for Research on Social and Economic Dimensions of an Aging Population (SEDAP), 2007/05, 36 p.
Are measures of health and economic activity comparable in european surveys?
Policies for reducing inequalities in health, what role can the healthcare system play? A European perspective. Part II: Experience from Europe.
Couffinhal A., Dourgnon P., Geoffard P.-Y., Grignon M., Jusot F., Lavis J., Naudin F., Polton D. 2005/02
Policies for reducing inequalities in health, what role can the health system play? A European perspective
Comparability of health surveys in Europe : France, United Kingdom, Russia, Poland, Czech Republic, Germany, Greece, Italy, Spain and Sweden."Indicators of active life expectancy" - Project International Longevity Centre, Barnay T., Jusot F., Rochereau T., Sermet C., Irdes/ILC, 2005/02/28.
Is the French Health Insurance an Efficient Instrument for Intragenerational Income Redistribution? Jusot F. , In "Journal d'Economie Médicale", vol.22, n° 3, 2004/05, pp. 99-118.
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text:
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text:
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Gunner and Murphy are a team on TNA. They are billed as a pair of security guards who have failed in the business and want to become recognized and successful in wrestling.
They are attempting to impress Ric Flair by co-operating with Fourtune throughout 2010.
On January 27, 2011 they teamed up with Fourtune and Immortal to assault Kurt Angle and Crimson.


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I am going to *try* to keep this short and to the point. Austin turned 4 December 9th. I'm worried that he isn't "up to par" with his age group nor that his tantrums are normal. He was born at 33 weeks into my pregnancy after I'd lost his twin brother. They'd started him on antibiotics after birth due to a fear that he had an infection in his brain caused by being carried with his brother for so many weeks after he'd passed. They told me to expect developmental delays, and the possiblity that it could be worse than delays. But going by his adjusted age, during infancy, he seemed to be flourishing.
Fast forward a few years and I'm now worried that he isn't as up to speed as he should be. His speech seems off to me. He doesn't seem to form sentences as much as other children his age. He mocks everything my mom, step-dad or I say, but he doesn't seem to grasp full sentences. Not to say he doesn't form them, but I don't think he's forming as many as he should be. I.e. if he can get by just saying a word or two, he will. He doesn't seem to draw like other children his age either. Doesn't try to form lines, color in the lines, or anything like it. Just scribbles. He's just recently gotten really good at using the potty. Just normal things other kids his age seem to be doing, he isn't.
His tantrums are beyond awful, I am not even certain they can be characterized as tantrums -- rather more full on mental breakdowns. I'd love to say I'm over exaggerating here but I've never seen a child throw a tantrum like this, ever. And I've been around a LOT of kids.
Two examples: I had to send my laptop back to HP for repair. I scheduled a pick-up date for the postman to come to the house and pick it up. He came to pick it up, I handed it to him and shut the door. Austin lost it. Kicking, screaming, crying, tossing himself around on the floor, and BANGING his head into the floor (on purpose mind you, this wasn't some accidental thing that happened while he was toss this outrageous fit, he purposefully slammed his head into the floor) at least twice before I got over to him to pick him up out of fear that he was going to cause himself serious harm. When I picked him up he BIT me. Then started trying to throw himself around again. I tried to calm him down and explain that the postman would bring it back after it was fix and he started all over again. Screaming "It never come back"
One day my step-dad (We're living with them while I work and finish school) moved a chair in the living room. Austin had the same kind of fit he did when the postman came to pick up the computer. Kicking, screaming, crying, tossing himself on the floor, and banging his head.
He does this any time something is moved, taken out of the house by someone other than himself, any time someone leave the house, any time our dog doesn't come in or out of the house when he wants her to. Just normal everyday things cause him to have these mini breakdowns. I don't know what to do, his pediatrician says not to worry about it, but I'm worried he's going to hurt himself.
I'm scared to send him to school this coming August, I won't take him to a daycare all because I simply don't know how he's going to react. I am at a loss here and feel like a complete failure.
Any advice?


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