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Page 236
Journal of the House of Commons: Volume 9, 1667-1687. Originally published by His Majesty's Stationery Office, London, 1802.
This free content was digitised by double rekeying. All rights reserved.
In this section
Sabbati, 15 die Aprilis, 1671.
London Ministers.
SIR Thomas Clergis reports from the Committee, to whom the Bill concerning the Maintenance of the Ministers of the City of London, was committed, several Amendments and Alterations, agreed to by the Committee, to be made to the said Bill: Which he read in his Place; and afterwards delivered the same in at the Clerk's Table: Which, being twice read, were, upon the Question, severally agreed unto.
Resolved, &c. That the said Bill, with the Amendments, be ingrossed.
The House then attended the Conference with the Lords, upon the Reasons of disagreeing with their Lordships to their Amendments to the Bill of additional Impositions on foreign Commodities.
And the Managers thereof report, that they had attended the Conference accordingly.
And then the House adjourned till Monday Morning, Eight of the Clock.


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A University of Georgia student recently showed me a book about Darwin's finches and proudly proclaimed it proved evolution was true. Wow, another giant leap for mankind - a book about how Galapagos finch beaks become enlarged or shrink when the seasons are dry or wet proves that rocks turned into people. That is a giant leap for sure. If evolution were even vaguely reasonable, can anyone tell me why, on Aug. 15, Harvard University announced it would be providing $1 million annually to researchers looking at issues regarding the origins of life?
Let's get right down to evolution's insurmountable problem: information. Rocks don't write poetry and lightning doesn't compose symphonies. Neither matter nor energy are capable of generating information. Sure, we can encode information onto matter, like beads on an abacus; or into energy, like radio and TV waves, but in the end, information can only come from a source of greater information. What or who was that source?
I certainly don't want creationism taught in our schools, when churches can't even agree on what happened or when. Intelligent Design, on the other hand, raises the kinds of questions that stir a child's natural curiosity and in the long run can only advance the study of all the sciences.
I recommend the book, "In the Beginning was Information" by Werner Gitt.
Terry Berg
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There are all sorts of positive things coming from the legalization of medical marijuana across the country. People who have been suffering from chronic pain for years are finally able to treat that pain effectively with medical marijuana, while others who suffer from stomach issues are able to get the treatment they deserve. Still other people with mental health issues like anxiety are finally able to treat that anxiety in an effective manner. On top of all of this states are finally able to tax and regulate an industry that's been on the black market for decades, which means more money for the states, more business, and less people going to jail for non-violent offenses. It's clear to people all across the country that the benefits of legalized medical marijuana far outweigh the negatives, of which there are little to none.
However, there are still lots of people out there who don't know very much about marijuana. Thus, when they're told by their doctor that medical cannabis might be something that could treat the issue that they're dealing with they aren't sure what their next steps should be. They get a prescription note (or a medical card in some states) and then are directed to a medical dispensary where they can purchase their marijuana. Many people get incredible nervous before they walk into a dispensary for the first time, and some people get so nervous and feel so uncomfortable that they can't even bring themselves to do it. Even if they are able to muster up enough courage to actually walk into the dispensary they aren't sure what they should be looking for once they get in there. That's why it's so important that people choose a medical marijuana dispensary like Greener Pastures that works hard to ensure that their customers feel comfortable. Their staff members greet each customer with a smile, ask them if they need help finding anything, go over their options with them, and help them find the best strain of marijuana for their particular situation.
Of course, for some people finding such a helpful dispensary isn't so easy. That's why it's important that people arm themselves with some knowledge about the marijuana plant before they walk into their first dispensary. They should do some homework and learn a little bit about the plant so that they know what they're looking for. The number one thing that you should know about marijuana is that you can learn a lot by looking at each cannabis bud closely. When you hold up each cannabis bud and examine it you can tell how many trichromes are on the plant and thus gain a little bit of understanding about how strong it's going to be. From there, you should ask the person working the counter what strains are in it. There are two main strains of marijuana, sativa and indica, and smoking them produces entirely different sensations. You should consult with your doctor and the dispensary about which strain would likely be best for your particular medical condition.


text: Posted November 4, 2006 On the way to work this morning I noticed that Detroit was completely froze over. Sallie was completely frozen this morning, but the wind opened up the section by Dunton Locks and that spread to most of the NE side of the lake. Height of Land was froze over yesterday afternoon , and Shell was about 75% froze as well. The warm weather that's forecasted is looking to undo much of what took a week of below freezing temps to accomplish. Hopefully the forecast will be wrong, and we'll be able to get on these lakes in a few weeks. Share this post Link to post Share on other sites


text: For more than 150 years, the UW — with the communities we serve — has demonstrated that an innovative spirit and commitment to the greater good can truly transform the lives of students and change the world. The UW is a partnership and a promise with our community that spans generations. It's a legacy that through the most expansive philanthropic campaign in the University's history is igniting an even stronger aspiration, energizing alumni, faculty and staff, and uniting our community.


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Keynote Speaker
Peggy speaks about the power each woman has to make a difference in ministry and in the world.
All About Influence: 2005 Women's Leadership Conference
- Turning Tragedy into Influence
- When Passion and Indifference Intersect
- Find Your Mission and Focus Your Influence
- Seasons of a Woman's Life
- Bringing Women Back from Alternative Spiritualities
- Nurturing Your Soul
- Working with Men and Making it Work
- The Balanced Life
- Becoming a Woman of Influence in the Workplace
- Influencing Your Community
- Resolving Conflict Biblically
- Bridging the Generational Gap through Mentoring
- Keynote Speaker
- How Do I Fit into My Husband's Life or Ministry Vision?
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I am still bright red, but the flaking has already started (day 4). First, my upper lip cleared but now I suddenly have developed two bumps. These are quite visible. Will these go away? Many thanks!
Bumps Post-Mixto SX CO2 Laser Treatment
Doctor Answers 4
Bumps days after laser resurfacing
Fractional laser resurfacing using Mixto, Total Fx, or Fraxel Repair can result in milia formation, where there is clogging of pores due to accumulation of dead skin cells. This is not unexpected and can be extracted later with minimal risk of scarring by an experienced board-certified dermatologist or plastic surgeon.
Learn more: http://www.drwilliamting.com/What_s_New.html
If skin irritation occurs after MiXto SX Micro Fraction CO2 Laser treatment
The bumps should be brought to the attention to your physician. Without seeing it or performing an examination, asking this question in any discussion forum is not likely to be helpful.
Bumps Post Laser Resurfacing Need A Physician's Examination
Any laser skin resurfacing can occasionally cause transient bumps or irregularities to the skin. That said, very rarely, these bumps can be related to a cold sore or infection. If these bumps are from a cold sore your cosmetic surgeon must evaluate you as soon as possible to avoid any untoward side effects.
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Try to be as patient as you can...
Hi- small bumps forming in the period after a Mixto procedure are common, and may be caused by some of the flaking skin clogging your pores, or the ointment you are using to help with your recovery.
In either case, as you heal and the flaking skin is gone, and as you transition to skin care that is less likely to clog your pores, these will clear. If they are worsening, or if the bumps are associated with significant and worsening pain, it's best to call your doctor as they may actually be indicative of a problem, but this is unlikely.
Congratulations on your new skin!
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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The Purple Sunset by CrystalisedXWatch
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Ok, so im going to sell out now and upload a classic picture of a sunset on the beach. I dont normally go for simple, easy-to-read images like this but this time i have an emotional connection to it considering its back in Greece where i spent half my life, no its only natural. Anyway, hope you like it.
wow...plain out... wow.....


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Evaluation of ES-derived neural progenitors as a potential source for cell replacement therapy in the gut
Stem cell-based therapy has recently been explored for the treatment of disorders of the enteric nervous system (ENS). Pluripotent embryonic stem (ES) cells represent an attractive cell source; however, little or no information is currently available on how ES cells will respond to the gut environment. In this study, we investigated the ability of ES cells to respond to environmental cues derived from the ENS and related tissues, both
Neurospheres were generated from mouse ES cells (ES-NS) and co-cultured with organotypic preparations of gut tissue consisting of the longitudinal muscle layers with the adherent myenteric plexus (LM-MP).
LM-MP co-culture led to a significant increase in the expression of pan-neuronal markers (βIII-tubulin, PGP 9.5) as well as more specialized markers (peripherin, nNOS) in ES-NS, both at the transcriptional and protein level. The increased expression was not associated with increased proliferation, thus confirming a true neurogenic effect. LM-MP preparations exerted also a myogenic effect on ES-NS, although to a lesser extent. After transplantation
This is the first study reporting that the gut explants can induce neuronal differentiation of ES cells
Several enteric neuronal disorders, such as achalasia, Hirschprung disease, congenital pyloric stenosis and pseudo-obstruction, are characterized by the loss or malfunction of critical neuronal subpopulations of the enteric nervous system (ENS)
Multipotent neural stem cells (NSC) isolated from the brain of embryonic mice can be transplanted in the gut of a transgenic model of gastroparesis and promote significant functional recovery as early as 1 week post-grafting
Embryonic stem (ES) cells can theoretically overcome many of these limitations and represent an alternative approach to neural precursors. ES cells are pluripotent cells derived from the inner cell mass of pre-implantation blastocysts
All experimental animal protocols were approved by the Institutional Animal Care and Use Committee at the University of Texas Medical Branch (Galveston, Texas) in accordance with the guidelines provided by the National Institute of Health.
Mouse ES cells culture
Mouse ES cells from 129/Ola strain (a kind gift of Dr. Jie Du) were maintained for no more than 25 passages in feeder-free conditions on gelatin coated flasks (bovine gelatin from Sigma-Aldrich, flasks from Nunc), in Glasgow's modified Eagle's medium (Sigma-Aldrich, St. Louis, MO) supplemented with 2 mM L-glutamine (Invitrogen Corp., Carlsbad, CA), 1 mM sodium pyruvate and 1X solution of non-essential amino acids (both from Sigma-Aldrich, St. Louis, MO), 10% fetal bovine serum (FBS; HyClone), 50 μM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), 100 μg/ml Primocin antibiotic (Amaxa Inc.) and 1000 U/ml LIF (ESGRO, Millipore). ES cells were sub-cultured using 0.05% trypsin/0.02% EDTA (Invitrogen Corp., Carlsbad, CA) in phosphate buffered saline (PBS) and plated at 25,000-30,000 cells cm-2 in freshly gelatinized flasks.
Alkaline phosphatase assay was carried out using the Alkaline Phosphatase Detection Kit (Millipore) according to manufacturer's instructions.
Induction of neurospheres from mouse ES cells
ES cells were trypsinized and plated at a density of 30,000 cells cm-2 onto non-coated flasks in neurobasal medium containing 2 mM L-glutamine, B27 supplement and 100 U/ml penicillin-streptomycin (NB27 medium; all reagents from Invitrogen Corp.), plus 1000 U/ml LIF, 10 ng/ml FGF2 and 50 ng/ml EGF (Promega). Cells were cultured for 7 days as floating multicellular aggregates termed ES-derived neurospheres or ES-NS.
Isolation and
Cell culture reagents were obtained from Invitrogen Co. (Carlsbad, California) except where noted. Staged-pregnant female GFP mice (TgN(GFPU)5Nagy, Jackson Laboratory, Bar Harbor, Maine) at embryonic day 15 (E15) were anesthetized with sodium pentobarbital (70 mg/kg, i.p.) and a midline incision was made to expose the embryos. The brains of embryonic mice were removed and the subventricular zone (SVZ) dissected from each brain hemisphere. The tissues were washed in Ca2+- and Mg2+ -free Hank's Buffered Salt Solution (HBSS) and digested using a combination of dispase type I (0.1%) and trypsin (0.005%) for 10 minutes at 37°C. After digestion, a cell suspension was obtained by gentle trituration, pelleted and resuspended in Neurobasal medium containing B27, 2 mM glutamine and penicillin-streptomycin (NB27), plus 20 ng/ml fibroblast growth factor (bFGF) and 20 ng/ml epidermal growth factor (EGF) (Promega, Madison, Winsconsin).
Differentiation of mouse ES-NS and CNS-NSC
To assess differentiation, multicellular aggregates were dissociated by incubation with Accutase (Innovative Cell Technologies, Inc., San Diego, CA) at 37°C for 10 minutes. Single cell suspensions were plated onto poly-ornithine (Sigma-Aldrich, St. Louis, MO)/mouse laminin (Invitrogen Corp, Carlsbad, CA) coated plates (BD FalconTM, BD Biosciences, San Jose, CA) in NB27 medium without growth factors and cultured for 7 days.
Co-culture with muscularis externa-myenteric plexus
Adult C57BL/6 J and TgN(GFPU)5Nagy mice (3-5 week old males, Jackson laboratories) were used. The TgN(GFPU)5Nagy mice have the Enhanced Green Fluorescent Protein (EGFP) driven by chicken beta-actin promoter and CMV intermediate early enhancer which drives GFP expression ubiquitously in all tissues. Mice were euthanized by cervical dislocation. The small intestine was dissected in pieces of 1 cm length and placed over a glass rod. A small incision was made and muscle layers with the adherent myenteric plexus were peeled off using cotton swab. The majority of preparations contained only longitudinal muscle and myenteric plexus and therefore we referred to them as LM-MP. LM-MPs were cultured at 37°C in 5% CO2 for 24 hrs in DMEM containing 10% FBS and 100U/ml penicillin-streptomycin (all reagents from Invitrogen Corp.). ES-NS were dissociated and plated onto poly-ornithine/laminin coated plates at a density of 2000 cells cm-2 in NB27 medium supplemented with 1000 U/ml LIF, 10 ng/ml FGF2 and 50 ng/ml EGF. Cells were cultured for 18 hrs before placing the LM-MPs into a 0.4 μm microporous insert (Corning Inc., Corning, NY, USA) inside each well (5 pieces per 24 mm-diameter insert). Co-cultured ES-NS were maintained in NB27 medium without growth factors and cultured for 10 days. Freshly prepared LM-MPs were placed in the inserts after 5 days. Control ES-NS were cultured in the same conditions without LM-MPs.
Flow cytometry analysis
Cells were dissociated using Accutase digestion according to manufacturer's instructions (Innovative Cell Technologies, Inc., San Diego, CA) and collected by centrifugation. 5 x 105 – 1 x 106 cells were fixed in ice-cold 4% paraformaldehyde for 15 minutes at room temperature (RT). Cells were then blocked and permeabilized for 5 minutes in PBS containing 0.1% Triton X-100 and 2% normal goat serum (NGS). Incubation with Ki67 antibody diluted in PBS containing 2% NGS was carried out for 30 minutes at RT, followed by incubation with secondary antibody for another 30 minutes (see Table
Real time PCR analysis
Total RNA was extracted from cells using the TRIzol kit (Invitrogen Corp.) according to manufacturer's instructions. First-strand cDNA was then generated using TaqMan Reverse Transcription Regents (Applied Biosystems, Foster City, CA, USA). Quantitative real-time polymerase chain reaction (PCR) was carried out in multiplex using the PTC 200 Peltier Thermal Cycler equipped with Chromo 4 Continuous Fluorescence Detector (MJ Research Waltham, MA, USA). PCR was performed using GeneAmp PCR kit and AmpliTaq Gold polymerase (Applied Biosystems) with the following cycling conditions: 2 minutes at 50°C, 10 minutes at 95°C, followed by 40 cycles of 15 seconds at 95°C, 1 minute at 60°C and fluorescence plate reading. The following sequence-specific primers and probes were designed using Primer Express software 2.0 (Applied Biosystems): β-III tubulin: Fw GGTCTGGCGCCTTTGGA, Rv AGTTGTTGCCAGCACCACTCT, probe Cy5-CCTATTCAGGCCCGACAACTTTATCTTTGGT-IBRQ; PGP9.5: Fw GCTTCGCCGACGTGCTA, Rv TTGCTTTTTCCTGAAGTTTTCATG, probe Cy5-CTGCTGCTCCTGTTTCCCCTCACG-IBRQ; peripherin: Fw GCATCTCAGTGCCGGTTCAT, Rv TGGGACTCTGTCACCACCTTCT, probe TexRed-TTGCCTCTCTAAGTTTAAAGACGACTG-IBRQ; nNOS: Fw GGGAAACTCTCGGAGGAGGA, Rv TGAGGGTGACCCCAAAGATG, probe 6FAM-CGTGGTACCGGTTGTCATCCCCTCAG-TAMRA; GFAP: Fw GAGGAGGAGATCCAGTTCTTAAGGA, Rv GCCTCGTATTGAGTGCGAATC, probe 6FAM-CCAGACCTCACAGCGGCCCTGA-TAMRA; α smooth muscle actin: Fw GAGACTCTCTTCCAGCCATCTTTC, Rv TGATGCTGTTATAGGTGGTTTCGT, probe 6FAM-ATGCCCGCTGACTCCATCCCAA-TAMRA. Ribosomial RNA 18 s (primers and VIC-conjugated probe from Applied Biosystems) was measured as a reference gene. Cycle Threshold (CT) values were quantified using Opticon Monitor 2 software (MJ research) and normalized calculating the mRNA level of each probe (x) relative to the amount of 18 s mRNA (reference gene) as follow: mRNA(x) = 2 CT (18 s) – CT (x) x 100. Mouse brain cDNA was used as positive control for all primers and probes and NO-RT reactions were used as negative control to check for genomic DNA contaminations.
Western blot analysis
Cells were dissociated and collected in pellets, which, after incubation overnight at -80°C, were lysed in ice-cold buffer containing 2% sodium dodecyl sulphate (SDS), protease cocktail inhibitor (Sigma-Aldrich), 1 mM phenylmethylsulphonylfluoride (PMSF), 1 mM dithiothreitol (DTT), 5 mM ethylenediaminetetraacetic acid (EDTA) in 50 mM Tris-HCl, pH 7.4. After centrifugation, the supernatants were collected and protein content was determined (BSA Protein Assay Kit; Pierce). Protein samples were processed for SDS-polyacrylamide gel electrophoresis (PAGE) with Mini-Protean Gel-3 system (Bio-Rad laboratories, Hercules, CA, USA). After electrophoresis, proteins were transferred to Polyvinylidene Difluoride (PVDF) membranes (Bio-Rad). Blots were incubated in blocking buffer (5% non-fat dry milk in TBS containing 0.1% Tween 20; TBST) for 1 hr at RT and probed with primary antibodies (Table
Immunofluorescence analysis
Cells were fixed in ice-cold 4% paraformaldehyde for 15 minutes at RT, blocked and permeabilized for 1 hr with PBS containing 0.3% Triton X-100 and 5% NGS. Samples were incubated with primary antibodies (Table
Cell counts were performed by counting the number of immunopositive cells for a given antibody and expressing the results as number of positive cells versus total cells number (determined using the nuclear counterstain DAPI that forms fluorescent complexes with natural double-stranded DNA and it is used to label the nuclei in all the cells). An independent investigator who was blinded to the treatment counted three regions per slides. A total of 3 experiments were counted.
Intra-pyloric transplantation
To enable the detection of the cells
One week following ES-NS transplantation, mice were deeply anesthetized with sodium pentobarbital (70 mg/kg intraperitoneally), transcardially perfused, and fixed with freshly prepared ice-cold 4% paraformaldehyde in 0.1 M PBS (pH 7.4). The pylorus was removed, post-fixed in 4% paraformaldehyde, and cryoprotected by infiltration in 20% sucrose solution in PBS overnight at 4°C. Tissues were rapidly frozen in O.C.T. embedding medium (Tissue Tek; Sakura, Tokyo, Japan) over dry ice–chilled isopentane (Sigma Chemical Co, St Louis, MO). Frozen serial sections (16 μm thick) were cut on a cryostat (TBS, Durham, NC), placed on Superfrost Plus slides (VWR, West Chester, PA), and stored at -20°C until needed.
Statistical analysis
RT-PCR and WB data were expressed as mean ± SEM. For comparisons between the groups One Sample
Characterization of murine ES cells
Characterization of the 129/Ola ES cell line was carried out by investigating common attributes of embryonic stem cells: colony morphology and alkaline phosphatase expression. Murine ES cells grown in feeder-free conditions formed colonies with the typical round and compacted morphology (Figure
Figure 1
Characterization of 129/Ola murine ES cells and of ES-derived neurospheres
Characterization of 129/Ola murine ES cells and of ES-derived neurospheres. (A) Phase contrast image of ES cell colonies with the characteristic compact morphology. (B) ES cells colonies show a high expression of the stem cell marker alkaline phosphatase. Calibration bars = 25 μm. (C) Phase contrast micrograph of ES-derived neurospheres (ES-NS) after 7 days in culture in serum-free medium supplemented with growth factors (NB27 + GF) and (D) immunofluorescence analysis showing the expression of the neural stem cell markers nestin (red). Nuclei are counterstained with DAPI (blue). Calibration bars = 25 μm. (E) Western blot analysis of nestin expression in ES-NS compared to ES cells grown under regular conditions. n = 3 ** P < 0.01 One Sample
Induction of neural progenitors from ES cells
In order to start with an enriched population of neural precursors we have modified existing protocols for generating ES-derived neurospheres
Some cells within the neurospheres also express the neural crest stem cell marker p75 and the neural progenitor marker βIII-tubulin. On the other hand, the mature neuronal marker PGP9.5 and the glial marker GFAP were not detectable in ES-NS (Figure
To further explore the neurogenic potential of ES-NS, neuronal differentiation of ES-NS was assayed after 1 week of culture in the absence of any growth factor and compared to that of uncommitted ES cells. Under these conditions ES-NS were able to generate neurons identified by a clear uni/bi-polar neuronal morphology (Figure
Figure 2
Neurogenic potential of ES-derived neurospheres
Neurogenic potential of ES-derived neurospheres. ES cells (A) and ES-NS (B) were plated out onto poly-ornithine/laminin-coated plates and allowed to differentiate by culturing them in NB27 medium without growth factors for 7 days. More βIII-tubulin-positive cells (in red) are visible in ES-NS cultures as compared to ES cells differentiated under the same conditions. Nuclei are counterstained with DAPI (blue). Calibration bars = 25 μm. (C) Western blot analysis for βIII-tubulin expression in differentiated ES-NS compared to differentiated ES cells. n = 3, **P < 0.01 One Sample
Interestingly, ES-NS did not generate astrocyte-like cells as indicated by the lack of expression of glial fibrillary acidic protein (GFAP), a marker of the glial lineage, as assessed by both Western blotting and RT-PCR (data not shown).
Small intestinal longitudinal muscle-myenteric plexus preparations
To investigate the effects of putative gut factors on the differentiation of ES-NS we established an
Figure 3
Time course analysis of small intestine longitudinal muscle – myenteric plexus (LM-MP) preparations
Time course analysis of small intestine longitudinal muscle – myenteric plexus (LM-MP) preparations. Immunoreactivity for the neuronal marker βIII-tubulin (red, A-B) and for the glial marker GFAP (red, C-D) reveal a preserved anatomy of the myenteric plexus and its ganglia (insets) in mouse small intestine LM-MP strips maintained in culture in NB27 medium for 5 days (B, D) compared to fresh preparations (A, C). Similarly, the persisting reactivity for α-smooth muscle actin, α-SMA (red, E-F) shows the longitudinal muscle still intact after 5 days in culture (F, fresh preparation control in E). Nuclei are counterstained with DAPI (blue). Calibration bars = 50 μm.
Neurogenic potentials of ES-NS in co-culture with LM-MP
When co-cultured with mouse LM-MP, ES-NS showed a significant increase in the mRNA expression of the neuronal markers βIII-tubulin, PGP 9.5 and peripherin as well as of neuronal nitric oxide synthase, nNOS, as compared to control ES-NS cultured without LM-MP (2.0, 1.3, 1.7 and 1.7 fold increases respectively, P < 0.05, n = 7; Figure
Figure 4
Co-culture of ES-NS with LM-MP preparations for 10 days leads to a significant increase in neuronal differentiation
Co-culture of ES-NS with LM-MP preparations for 10 days leads to a significant increase in neuronal differentiation. Expression of neuronal and glial markers was assessed by RT-PCR (A), Western Blot (B) and immunohistochemistry (C). The pan neuronal markers βIII-tubulin and PGP9.5 and peripherin, as well as the neuronal subtype marker nNOS were increased both at the mRNA and protein levels in the LM-MP co-cultured group as compared to control (fold change ranging from 1.3 to 3.2). The glial marker GFAP was not detected at this stage of differentiation by any of the analysis performed. (A) Changes of mRNA level in ES-NS co-cultured with LM-MP as compared to control cultures (ES-NS without LM-MP). n = 7. * P < 0.05, ** P < 0.01. (B) Changes in protein level in ES-NS co-cultured with LM-MP as compared to control cultures. n = 6. * P < 0.05, ** P < 0.01. (C) Changes in percentage of positive cells in ES-NS co-cultured with LM-MP as compared to control cultures. n = 3. * P < 0.05, ** P < 0.01. (D-E) Representative images of immunoreactivity for PGP9.5 (green), a marker for mature neurons, in control cultures and ES-NS co-cultured with LM-MP. PGP9.5 immunoreactivity extends to the neurites (arrows, E) although it is less intense partly due to the difference in focal planes. Scale bar = 25 μm. (F-G) Representative images of immunoreactivity for nNOS (red) in control cultures and ES-NS co-cultured with LM-MP showing increased proportion of nNOS+ cells after co-culture with LM-MP compared to controls. Scale bar = 50 μm. Nuclei are counterstained with DAPI (blue).
Immunofluorescence analysis corroborated these findings, revealing a significant increase of percentages of cells positive for βIII-tubulin, PGP9.5 and nNOS after exposure to LM-MP compared to control cultures (βIII-tubulin 42.3 ± 7.0% vs 23.6 ± 6.3%, PGP9.5 39.9 ± 6.6% vs 21.6 ± 4.7%, nNOS 15.8 ± 3.5% vs 11.8 ± 4.6%, with 3.2, 2.3 and 1.6 fold increases respectively, P < 0.05, n = 3. Figure
Figure 5
Differentiated neurons in LM-MP treated ES-NS exhibit a more complex and mature morphology than control neurons
Differentiated neurons in LM-MP treated ES-NS exhibit a more complex and mature morphology than control neurons. Morphology of neurons is shown by immunoreactivity for the neuronal markers peripherin (red, A-B) and βIII-tubulin (red, C-D) in ES-NS co-cultured with LM-MP (B, D) or control (A, C). Neurons arising from ES-NS exposed to LM-MP show pronounced processes and form a network-like structure, whereas control neurons show either absence of neuritogenesis (A) or shorter blunt processes (C) white arrowheads. Nuclei are counterstained with DAPI (blue). Calibration bars = 25 μm. (E) Analysis of the expression of the cell cycle marker Ki67 by flow cytometry does not reveal any significant change between the two groups. n = 3, P = 0.81, Student's
We also demonstrated that increased cell proliferation is unlikely to account for elevated levels of neuronal markers since the percentage of Ki67 expressing cells is similar in the co-culture and control groups (6.1 ± 1.2% and 5.6 ± 1.2% respectively, P = 0.81, n = 3; Figure
Immunofluorescence analysis also revealed the appearance of a small percentage of cells (less than 1% of the total) positive for smooth muscle marker α-SMA in the co-cultured ES-NS (Fig
Figure 6
Smooth-muscle actin expression in ES-NS
Smooth-muscle actin expression in ES-NS. α- SMA immunoreactivity (red) in ES-NS cultured in the presence (A) or absence (B) of LM-MP. A small percentage of α-SMA positive cells were generated from LM-MP treated ES-NS as compared to the control group where they were rare. Nuclei are counterstained with DAPI (blue). Calibration bar = 25 μm. (C) RT-PCR analysis confirmed the increase of α-SMA transcript in LM-MP treated ES-NS as compared to control (n = 6, ** P < 0.01). This result was corroborated by Western Blot analysis shown in panel D (40 μg of total protein extract per lane. Loading control GAPDH, Glyceraldehyde 3-phosphate dehydrogenase), indicating increased expression of α-SMA in the LM-MP co-culture group as compared to control where the expression of α-SMA was negligible.
Intrapyloric transplantation of ES-derived neurospheres
To assess preliminary feasibility of ES-derived neurospheres transplantation, we used the experimental protocol of intrapyloric transplantation previously described for CNS-derived neural stem cells (CNS-NS)
Figure 7
Intrapyloric transplantation of ES-NS
Intrapyloric transplantation of ES-NS. (A) Grafted DiI-labeled ES-NS (red) after 7 days from transplantation were found mainly in the muscle and submucosal layers. (B-D) Immunofluorescence staining revealed a failure by the grafted ES-NS to express mature phenotype markers such as peripherin (green, B), GFAP (green, C) and α-smooth muscle actin (green, D). Nuclear staining with DAPI (blue). M, muscle; SM, submucosa; Mu, mucosa. Calibration bars = 25 μm.
Although ES cells can differentiate into neural precursors in the absence of any external influence, only a small percentage of neural cells survive and differentiate under these conditions
The neurogenic effect observed after co-culture of ES-NS with LM-MP could be attributed to either increased proliferation and/or improved differentiation of neural progenitors within ES-NS. Our data show that the number of neurons (PGP9.5 and nNOS positive) is increased after co-culture with LM-MP, but not the number of proliferating cells (Ki67 positive). Moreover, co-culture with LM-MP clearly affects the morphology of neurons produced, which possess longer axons and form a more extensive and organized network as compared to controls. This suggests that soluble factors released from the LM-MP promote survival and differentiation of neural progenitors but not their proliferation. Therefore the main effect in our ES-NS co-culturing protocol is to favor differentiation of neurons.
In addition to inducing neuronal differentiation, we also observed a minor myogenic effect of LM-MP on ES-NS. Previous reports have shown that CNS-derived NS can be induced to express smooth muscle markers
ES-NS failed to express the glial marker GFAP under any of our culture conditions, unlike other studies using ES-derived neural precursors, which have reported the capability of these progenitors to differentiate into both the neuronal and glial lineage
It is well known that the environment plays a key role on the differentiation of ES cells into defined phenotypes. We have developed a robust and informative
Although transplantation of ES-NS in the pyloric muscle layer of wild type mice showed adequate survival of grafted cells at 1 week, no markers of terminal differentiation were noted. This contrasts with many reports that showed successful transplantation of ES-derived neural precursors in the CNS
We have previously shown that CNS-derived NS grafted in the pylorus of mice generate neurons and glia and partially restore gastric function
In this regard, our study had several limitations. First, it should be noted that the time frame of these experiments was relatively short. Long-term survival and differentiation of grafted cells needs to be investigated in further studies to fully assess the potential of ES-NS to form enteric neurons
A variety of cell sources are currently being explored for use in cell transplantation studies including CNS-derived NS, neural crest stem cells (NCCS), enteric neural progenitor cells (ENPC) and embryonic stem cells (ES)
Competing interests
The author(s) declare that they have no competing interests.
Authors' contributions
VS carried out the experiments, performed the statistical analysis and drafted the manuscript. MAM participated in the design of the study, in the statistical analysis and in the drafting of the manuscript. KMK performed cell counting and participated in the cell transplantation experiments. PJP conceived the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
Authors' information
The current affiliation of VS is Division of Molecular Neurobiology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill London NW71AA, UK.
The current affiliation of MAM is Department of Anesthesiology, University of Texas Medical Branch, Galveston, TX, USA.
The current affiliation of PJP is Johns Hopkins Center for Neurogastroenterology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
The authors would like to thank Dr. Jie Du for providing the 129/Ola ES cells and Dr. Tor Savidge for valuable support with the RT-PCR assay.
This study was supported by a grant from the National Institute of Health (DK62834 and DK80920) and from the Moody Foundation, Galveston, TX.
The enteric nervous system: normal functions and enteric neuropathies
New insights into human enteric neuropathies
Review article: molecular, pathological and therapeutic features of human enteric neuropathies
Future horizons in the treatment of enteric neuropathies
Neural stem cell transplantation in the enteric nervous system: roadmaps and roadblocks
Neural stem cells express RET, produce nitric oxide, and survive transplantation in the gastrointestinal tract
Neural stem cell transplantation in the stomach rescues gastric function in neuronal nitric oxide synthase-deficient mice
Caspase inhibition increases survival of neural stem cells in the gastrointestinal tract
Enteric nervous system stem cells derived from human gut mucosa for the treatment of aganglionic gut disorders
Establishment in culture of pluripotential cells from mouse embryos
Development of neuronal precursor cells and functional postmitotic neurons from embryonic stem cells in vitro
Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease
Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model
Generation of graftable dopaminergic neuron progenitors from mouse ES cells by a combination of coculture and neurosphere methods
Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells
Differentiation of mouse embryonic stem cells into a defined neuronal lineage
Directed differentiation of telencephalic precursors from embryonic stem cells
Generation of cerebellar neuron precursors from embryonic stem cells
Motoneurons derived from embryonic stem cells express transcription factors and develop phenotypes characteristic of medial motor column neurons
Neural differentiation of mouse embryonic stem cells
Neural differentiation from embryonic stem cells: which way?
Generation of neural crest-derived peripheral neurons and floor plate cells from mouse and primate embryonic stem cells
Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells
In vitro formation of enteric neural network structure in a gut-like organ differentiated from mouse embryonic stem cells
Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF)
Nestin expression--a property of multi-lineage progenitor cells?
Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism
Embryonic stem cells assume a primitive neural stem cell fate in the absence of extrinsic influences
Neural crest stem cells persist in the adult gut but undergo changes in selfrenewal, neuronal subtype potential, and factor responsiveness
Coculture of embryonic stem cells with pulmonary mesenchyme: a microenvironment that promotes differentiation of pulmonary epithelium
Directing epidermal fate selection by a novel co-culture system
Ontogeny of the neuronal intermediate filament protein, peripherin, in the mouse embryo
Lessons from genetically engineered animal models. IV. Nitric oxide synthase gene knockout mice
Functional development of the enteric nervous system–from migration to motility
The projections of early enteric neurons are influenced by the direction of neural crest cell migration
Differentiation of cholinergic cells in the rat gut during pre- and postnatal life
Contractile responses of smooth muscle cells differentiated from rat neural stem cells
Cell lineage analysis in neural crest ontogeny
The development of neural stem cells
Neural stem cells for the treatment of disorders of the enteric nervous system: strategies and challenges
Pre-publication history
The pre-publication history for this paper can be accessed here:


text:
Monday, November 14, 2011, 5:30 pm
Wray Finney Building, in the Mustang Room
1415 North 7th Street, Fort Cobb, OK
1. Call to Order, roll call and establishment of a quorum
2. Introduction of Visitors
3. Instructional Leaders: Kem Crowell, Christi Smith, Jack Whitman and David Darnell to give Reports & Updates
Asst. Superintendent: Wade Walling – presentation on Character First.
4. Superintendent's Reports and Anticipated Discussion by Board and Staff:
Perkins Consortium
Oklahoma Business Perspective Service Survey
Career Pathways Project
Oklahoma Academy of State Goals
Economic Development meeting on Campus Nov 22nd
Oklahoma Conservation Water Plan
Board Member Catch up Sessions
School Redistricting approved by State Board
5. Consent Agenda
All of the following items, which concern reports and items of a routine nature normally approved at board meetings, will be approved by one vote unless any board member would like to remove an item for individual consideration. The consent agenda consists of the discussion, consideration and approval of the following items:
a. October 10, 2011, Regular Board Meeting Minutes and October 24, 2011 Special Board Meeting Minutes
b. Appropriations for FY10-11, warrants, encumbrances for General Fund #638 - #783 and #70231 - #70255 in the amount of $548,920.61; Building Fund #9 - #15, in the amount of $28,889.75 including change orders and appropriations for FY11-12 from reports dated October 31, 2011.
c. Treasurer's reports for bank statement dated October 31, 2011
d. Activity Fund report for bank statement dated October 31, 2011
e. Transfer of $ 118,205.65 from the Activity Fund to the General Fund per resolution 70 O.S. § Section 5-129
6. Action to be considered:
a. Discuss and vote to approve or disapprove CKTC's Technology Plan FY11-12.
b. Discuss and vote to approve or disapprove the CKTC Board of Education to call for an annual
Election for school board seat Number Two (2), Zone Four (4) to be held February 14, 2012
c. Discuss and vote to approve or disapprove the possible closing of precincts with low voter registration.
d. Discuss and vote to approve or disapprove Requisition #678 in the amount of $11,348.00 to Western Technology Center and #680 in the amount of $35,756.00 to Western Oklahoma State College for Post-Secondary Services professional service funding.
e. Discuss and vote to approve or disapprove the CKTC Board Meeting Dates and Time for 2012
f. Discuss and vote to approve or disapprove HOSA class to sponsor food drive on campus to support local families.
g. Discuss and vote to approve or disapprove out of state travel to Savannah, GA for the Superintendent.
h. Discuss and review Superintendent Evaluation instrument.
i. Discuss, review and vote to approve or disapprove Certified Salaries/Scale adjustments.
j. Discuss, review and vote to approve or disapprove limited Classified Salaries.
k. Discuss and review High Demand Adjustment Pay Scale Policy.
i. Discuss, review and vote to approve or disapprove quotes for plumbing work in pre-engineering building #200.
7. New Business – This is limited to matters not known about or which could not have been reasonably foreseen prior to the posting of the meeting agenda
8. Proposed Executive Session.
To discuss the following personnel items so the board may return to open session to report on and/or take possible board action on the following personnel item Pursuant to 25 OS§ 307(B)(1).
a. Employment of Agriculture Business Management Coordinator
9. Motion, discussion, and vote to convene into Executive Session.
10. Board President's statement regarding Executive Session.
11. Motion, consideration and vote to approve or disapprove the employment of Agriculture Business Management Coordinator
12. Announcements
13. Adjournment
Note: "The Board may discuss, make motions, and vote upon all matters appearing on this agenda. Such votes may be to adopt, reject, table, reaffirm, rescind, or take no action on any agenda matter."
POSTED: Administration Building – Caddo Kiowa Technology Center
November , 2011, and 4:15 p.m.
Sharon Myers, Minute Clerk
Caddo Kiowa Technology Center Board of Education


text:
The adidas Predator Pulse was released in 2004 featuring a leather upper with rubber striking elements. The split sole design offered both soft ground and firm ground bladed studs. To see more pictures and video click the link above.
Adidas Predator Pulse Video Review
2 Comments
Hey Josh, i was wondering about the sizing for this model. Do they run true to size?


text:
|Popularity rating
|3 stars
|Large Herbivores
|Security requirements
|Dig site
|Morrison Formation
This is the smallest member of the sauropod family in Jurassic Park. Its mouth is full of teeth like small chisels which allow it to mash up the toughest plant material. Like other sauropods, they can forage for food at ground level and higher.[1]
Hints & Tips Edit
Camarasaurus are friends with the Brachiosaurus and is sometimes the prey to a pack of Velociraptors, Allosaurus , and Ceratosaurus .
References Edit
- ↑ Dinopedia/Camarasaurus.


text:
The remark property comes from the German remark "trost" which system comfort. Trust is having the self-confidence or creed in other person to do what they say they will do. It mechanism basic cognitive process in the righteousness and the steadfastness of that other mortal. Trust is a spirit ingredient of any empathy and a essential factor in control. There are cardinal middle components of trust. These are:
Competence. This is how economically you "appear" to be at you given job or odd job. Imagine active to a dentist's place of business for a fang activity and the dental practitioner has to name to a gradual go in front on how to delete your bone. Doesn't product you cognisance too comfy does it? Likewise, you can also be seen as an feckless colleague or person too. You can be seen as qualified by jutting authority and having cognition of the problem.
Respect. In writ for material possession to be established, one wishes to amazement the boundaries of the other than human being. This agreement creates a jelled starting point upon which further agreements can be improved. Respect should be self-reliant of whether or not you same the soul. You can contemn the soul for what they are, resembling near a sequential criminal or youth molester, but you have need of to distribute them approval as a quality person. Perhaps partly or more of the empire you agreement with on a day by day basis you wouldn't perfectionism to cognize on a own level, but that does not close-fisted that your of your own vibrations around that character should bewilder the magnitude of niceness and reverence that you appearance them. Respect builds rapport which creates belongings.Post ads:
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Appearance. You have to manifestation the division if you are going to set up immediate property near a causal agency or consumer. If you amble into the business office of the economic advisor you poverty to let to activity set up your pecuniary forthcoming and he is clad in woman's lingerie; would you let him? Your surfacing creates the situation for trust to take place. If you are busy in commercial afterwards you essential face professional. If you are sounding for holding in a relationship, past you will have to clothes to meeting their fashion.
Positivity. We all cognize the individual who complains going on for how bad his enthusiasm is and how he hates the worldwide and everything in it. This cognition is harmful to holding. People want to perceive affirmatory material possession like-minded wished-for goals and dreams because that inspires them and they can feed off that optimistic physical phenomenon. Always be optimistic in time and you will insight that it is easier to institute interaction and put up property.
Responsibility. There are no correct folks out here in the international and all and sundry makes mistakes. People know this and take to mean that it is portion of the basic cognitive process system of energy. Trust is improved on the footing that you income what you have to do for your mistakes in duration and larn from them and do your fastest to put right the sabotage caused by the misapprehension. By denying obligation for the error or projected the liability distant from yourself, you are essentially informative family that you haven't erudite from the misapprehension and that you won't assistance to put back together the break and that it will in all likelihood come about over again in the future.Post ads:
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Listening. People have a long for to be heard-for their ideas to be common by another people, so an easy way to erect belongings is to have an expand ear. Let the party tell to you just about their duration and the substance that is beta to them. This will have a correlative cause of artwork you into their lives and structure property.
Empathy. This is your propensity to see the global finished their persuasion by "walking in a circle in their situation." Empathy reduces the plane of stiffness linking some of you and facilitates material possession site through shared conception. (Refer to next subdivision for much on sympathy.
Honesty. You want to be based on fact in both your of our own and your professional life span. Honesty facilitates material possession place by letting your opposite number cognize that what you are telling them is truthful and sincere. A trusty tie is one where on earth the someone will venture fashioning the other personality distressed by telltale him thing that they may not poorness to comprehend if they cognize it will give support to them out in the perennial run.
Patience. People don't relish individual rushed through with things. Patience equals support and relief facilitates belongings. Giving a punter full amount of instance to believe material possession finished instead than pressuring them to wave the contract is an sample of mistreatment patience to habitus material possession.
Common interests. Having common interests provides you with a forum upon which you have a reason to collaborate and change over accepted wisdom. The more interests you cut the bigger. Common interests are the optimal way to run into citizens because it's something that both of you cognise and it's thing that both of you enjoy.
Enthusiasm. This is your rank of motive to production holding occupation out. People want to be say different individuals who are intended to forming decent relatives. If you collect being for the firstborn incident and they furnish you the "shrug-off" or are looking on all sides the area piece you are conversation to them, then that does not impart substantially appetite to proceed chitchat to them. However, if you group a somebody and they filch a ready flavour in what you have to say by actively attentive and maintaining correct eye-contact, afterwards you surface same they are spirited something like you and your duration.
Loyalty. A trustworthy affiliation is one where you can tell on the remaining soul beingness reliable to their language unit. A causal agent who is reliable ever holds their end of a declare. They have a soaring smooth of state to do the things that they say they will do.
Good interface. In decree for trust to come into flower in any given relationship, at hand has to be a smashing string of interested relations involving parties. Good memo involves production firm that your philosophy are accurately "faxed" all over to your opposite number and that miscommunication is avoided. This involves exchanging message betwixt each other and over-communicating instead than under-communicating. It channel telling the being how you cognizance and sending your interests, values, and goals so the other individual can know you advanced. Good relations involves the competency to blatantly handle conflicts and issues that are inevitable on the street towards interaction. The other portion of virtuous communication is state able to publication and declare best organic structure jargon. You deprivation to be confident you are sending communicatory vernacular that is even with your uttered spoken communication.
Security. According to Maslow's Hierarchy of Needs, ancestors have a have need of to cognisance sheltered and secure in their situation. You impoverishment to help assist that obligation for collateral by projecting yourself as a undisruptive and reassuring individual. You can do this by presenting yourself as non-threatening, intellectual, responsible, and sympathetic.
Self-sacrifice. Self-sacrifice is one of the most of value components of a sure affinity. Does the mortal forfeiture their own riches to aid you out, beside teensy or no gain for themselves? If picture becomes you antagonistic them, will they swirl on you to let go their own skin? You would be stupefied at the numeral of stop friends you have who would elbow you into the conflagration in direct to prevention themselves.
Copyright © 2007 by Tristan Loo. All rights silent.


text:
Mr. Ronen Ifergan
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No approved endorsements
About Mr. Ronen Ifergan
Ronen Ifergan is an Israeli Artist with Mediterranean, European and American influences that are incorporated into a modern approach of creating surroundings that are current and classical at the same time.
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Ronen's years of experience in the graphic design and fine arts will give your project a fresh and mature look with a surprising sense of eureka.
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KELLY ROBYN MANN Photographer
Lisa G ArtistryMakeup Artist Female Los Angeles, California, US
My Website: Lisa G Artistry
Mayhem # 687007
About Me
Thanks for checking out my makeup port on Model Mayhem!!!!
For more of my work check out www.LISAGARTISTRY.com or check me out on Face Book...
Have you worked with Lisa G Artistry? Add Credits for Lisa G Artistry! >>
KELLY ROBYN MANN Photographer
HairByNico Hair Stylist
Rachel Brumbaugh Hair Stylist
Cass Cunningham Photography Photographer
Chase Stanley Photography Photographer
Orada J Photography Photographer
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It doesn't look like much from the outside but we were pleasantly surprised that the Budget Inn was clean and newly remodeled. Staff was very friendly during check in. Pool is very basic with no shade but it looked clean. It was nice to have...More
Getting you more information on this room
Getting you more information on this room
"microwave, mini refrigerator, and a hairdryer are great luxury items in the room"
"I always prefer rooms on 2nd floor so you don't hear people above you."
"A room towards the back is away from the highway. Upstairs in the 2 storey part means no noise from above."


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Mumsnet has not checked the qualifications of anyone posting here. If you have any medical concerns we suggest you consult your GP.
Weight gain thyroid post birth-dose15stonw(1 Post)
Rox19 Wed 04-Jun-14 22:40:41
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Clichy slams sloppy Gunners
Arsenal defender Gael Clichy has hit out his team-mates for failing to see off Liverpool at Anfield.
Last Updated: 09/04/08 11:48am
Arsenal defender Gael Clichy has hit out his team-mates for failing to see off Liverpool in their UEFA Champions League quarter-final.
The Gunners looked to be on the verge of the semi-finals on away goals courtesy of Emmanuel Adebayor's equaliser to make it 2-2 six minutes from time.
However, Liverpool hit back with Steven Gerrard's controversial penalty and Ryan Babel's last-gasp goal sealing a thrilling 4-2 win for Rafa Benitez's side at Anfield.
Clichy was left fuming by the decisive penalty after Kolo Toure was adjudged to have upended Babel in the box.
The French defender was furious his side could not hold on to their overall lead after Adebayor had given them the advantage.
Disappointing"We did well to score the first goal and to come back and get the game to 2-2. And at that point we were through. But what happened after that was really disappointing," Clichy told the London Evening Standard.
"To concede a goal like this, whether it is a penalty or not a penalty, may be disappointing but it is not unlucky.
"It is unreal to have had a chance like this, to score the equaliser and get to 2-2 and then to concede a goal only a few seconds after kick off.
"I can't find the words for it. It is just ridiculous."


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All of us know allium sativum which is utmost normally used as a flavoring or a flavorer. You any like it or repugnance it once you see it on or stink it from your supplies. Garlic, whether raw or cooked, is celebrated for its passionate highlight olfactory property. But, are you aware that allium sativum can be nearly new medically besides its cooking purposes. In fact, allium sativum has long-term been well thought out as a flavorer "Wonder Drug".
When powdered or delicately chopped, allium sativum yields allicin, a almighty bactericide and anti-fungal smooth (phytoncide). It too contains ajoene, allicin, enzymes, victuals B, minerals, and flavonoids.
Ajoene, an unsaturated disulfide, is formed from the attachment of 3 allicin molecules. The rugged odour and feel of allium sativum comes from Allicin. After a allium sativum flower bud is pounded or fine chopped, the set free of allicin occurs. When allicin is liquid in assorted solvents with digestible oils, ajoene is definite. Ajoene can as well be saved in alliaceous plant citation. Ajoene is most stable and utmost lavish in separate of garlic (chopped allium sativum in pareve oil).
Scientists have latterly found ajoene has antithrombotic (anti-clotting) properties, which helps hinder platelets in the liquid body substance from forming humour clots. These properties can possibly cut back the venture of suspicion unwellness and shot in group.
There are at least 12 healthy designed studies published about the world that support that alliaceous plant in various forms can cut cholesterin. The greatest scrutiny so far was conducted in Germany where on earth 261 patients from 30 mass practices were specified any garlic soil tablets or a placebo. After a 12 week psychoanalysis period, suggest bodily fluid sterol levels born by 12% and triglycerides dropped by 17% in the allium sativum fumed faction compared to the medication body.
Scientists found that allicin blocks the enzymes by reacting near one of their high-status components famous as sulfhydryl (SH) groups, or thiols, which are also crucial components of quite a few enzymes that join in the blend of sterol. By reacting with and modifying the sulfhydryl groups in those enzymes, allicin may obstruct the harvest of artery obstructive cholesterin. This could impart a practicable elucidation for how garlic lowers the levels of toxic sterol.
Nevertheless, it is believed that garlic does have side effects. Due to its ironlike nature, raw allium sativum should not be eaten in full-size body as it could send out problems, for occasion anger of or even weaken to the biological process geographic area. Also, in attendance are a few general public who are allergic to allium sativum. Symptoms of garlic allergy consist of skin texture rash, fundamental quantity and headaches. Also, allium sativum could possibly stop anti-coagulants, so it is privileged avoided before medical science.


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Retro Super Future Flat Tops Delivery Available Now
Friday Jun 13 2014
Retro Super Future prepares you for summer with this collection of the Flat Top silhouette. The delivery features a cameo from legendary and creative artist Keiichi Tanaami, inspiration from bikers' rebellious lifestyle, and the use of smokey acetate. Each sunglass has its own unique design on the inner frame that sets them apart from one another. Be sure to pick up a pair and stay shaded during the hot summer season ahead. Phone Orders 702-463-3322 or email@example.com. International Shipping available.
-Kevin Sanchez
[nggallery id=1868]
-Kevin Sanchez
[nggallery id=1868]


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Colder air returns with burst of rain/snow showers
Sunshine gives way to cloud cover from west to east this afternoon, as the first of several "clipper" systems arrives in Central Indiana.
Enjoy what time we have left in the 40s with temperatures tumbling into the teens by 7am Wednesday.
A cold front will deliver a burst of rain/snow showers this afternoon… arriving around 2pm in western Indiana and 4pm for the Indy metro area.
Colder air from top to bottom allows precipitation this evening to be frozen, with flurries and snow showers erupting in the cold air advection. Some of the showers may be strong enough to produce localized accumulation and with lows in the teens some slick spots are very possible for the Wednesday morning commute.
A bigger story tomorrow morning will be single digit to near zero wind chills… courtesy of a brisk northwest wind at 10 to 15mph.
Suggested Links
A northwest flow aloft serves as a conveyor belt to transport multiple Albert clippers, with varying degrees of intensity. Timing and location will prove to be challenging between now and Saturday, but a few inches is more than possible by Sunday morning.
Though temperatures climb back into the mid 40s early next week, long range indicators suggest another polar plunge arriving by the end of the month. Have a great afternoon and thanks for visiting the site – Sean Ash


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Happy New Year!
Welcome back everyone. Happy New Year! We hope you all had a wonderful Christmas.
Our Lady of Compassion Primary School welcomes you! Please feel free to look around and see the fabulous things we do.
Mrs M.M. Lynn - Headteacher
Tel: 01704 877281
At Our Lady of Compassion Catholic Primary School we aim to provide the best possible educational and pastoral care for all our pupils. We strive to create opportunities to ensure children maximize their full potential. We aim to pass on an awareness and appreciation of the Gospel values and support all members of our school community in the name of Jesus Christ.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Field width overlaps form areaAsked by CatherineYN on May 15, 2015 at 11:31 AM
I want my form field max 645px and two of my fields are crossing the form width. Its a radio button. How can i fix it? it tired everything. Please help
JotForm Support
I cloned your form for testing purposes, I implemented my changes here: http://form.jotformpro.com/form/51345867830966. Could you check if my cloned form gives you a better result, if it does, you can clone it instead by following this guide: http://www.jotform.com/help/42-How-to-Clone-an-Existing-Form-from-a-URL.
These are the things I changed:
1. I set all the fields to have "Label Align" to "Top".
2. I set the Preferences->Responsive Form to "No".
3. Do not disable the "Make this form responsive" in the Form Designer tool. Leave it as enabled.
Try checking if this gives you a better layout in your form preview.
Do let us know if you need more information on this.
Thank you.
It was fix but again same issue and i did what you said.
My form: http://form.jotformpro.com/form/51303788386968
This field:
Which of the following best fits your impression of the Jameson Graduate Programme? *
Please can you prioritise the most important factors when considering a graduate programme. *
Still not responsive it's cutting off on mobile.
Please help.
Hi there;
Could you please post screenshot here, we have tested this form on Android device, it is working fine, please take a look to the screenshot below;
Do let us know, if you need further clarification.
Here are the screenshots.
Below one is from my site where i embedded this form:
Website link: http://collegetimes.com/2015/05/20/would-you-work-for-jameson
Please help.
Hi there;
Thankyou for the screenshots, could you try to add the following CSS Code Block to resolve this issue, please read our guide here How to Inject Custom CSS Codes.
.form-line-column {white-space: normal !important;}
I hope it will all-set! Kindly let us know further, if you have questions/queries, we would be happy to assist you.
Thanks its solved now :)


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Global Competition
Looking for a long read this long weekend? Check out Bruce Katz and Jennifer Bradley's article on the keys to creating collaborative metropolitan areas that can successfully compete in the global economy.
Next American City
Celebrate where you came from or where you're going with city t-shirts, jewelry, and neckwear.


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Reincarnation is the heretical belief held by infidels and many foreigners. It states that when you die you don't go to see the Baby Jesus, but instead return to America's planet as some other life form. What you come back as depends on your karma score.
Ontological fallacy of reincarnation Edit
- If reincarnation existed why don't Buddhists come back as something totally awesome and convince us they're right, so we can all be saved and the world can become a better place?
- Nixo facto reincarnation is nonsense!
Known thine enemy! Edit
When confronting reincarnation nuts it's important to know how they think. That way you can defeat them by dazzling them with your vast and superior knowledge of their false religion. Of course, just stamping on a passing ant will also crush their morale and bring them to tears. Either strategy is good.
Here is an example of how karma scores (which range between 600 and 2400) equate to what lifeform you are reincarnated into, if reincarnation were real, which it's not!
- 2400: Stephen Colbert's intern
- 2301-2399: Bush family member
- 2251-2300: Truthiness monkey
- 2201-2250: Colbert Nationalist
- 2101-2200: Honest, God-fearing red-state Republican
- 1701-2000: Saginaw Spirit mascot
- 1501-1700: Elephant
- 1401-1500: Dolphin
- 1301-1400: Independent-voting American
- 1201-1300: Non-French European
- 1001-1200: Cat
- 801-1000: Cockroach
- 751-800: Liberal
- 701-750: French person
- 651-700: Liberal
- 601-650: Terrorist
- 600: Bear


text: Online sports bettor shares positive feedback By SBR Saturday, April 23, 2016 Share with: An online sports bettor has shared positive feedback on his recent experience turning a $25 free play into a $151 cash payout. The player documented his feedback at the SBR Sportsbook & Industry forum, a sub-forum dedicated to sportsbook payout discussion, complaints, and reviews from online sports gamblers. According to the player, a betting website gave him $25 to try out their site and he completed a 5X rollover or play-through requirement before eventually requesting and receiving a $151 cash payout. Sportsbook Review encourages feedback from all bettors, good and bad, on their experiences with the websites that are included in the betting sites rating guide. Over 200 online sportsbooks hold an SBR rating ranging from A+ to F. Sportsbooks rated from D- to F are part of the worst sportsbook blacklist and should be avoided: these betting sites are synonymous with slow and no-pays. The April 2016 payout reports thread contains feedback from players on how long it took them to receive payments from betting sites.


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[ https://issues.apache.org/jira/browse/PHOENIX-3511?page=com.atlassian.jira.plugin.system.issuetabpanels:comment-tabpanel&focusedCommentId=15713004#comment-15713004 ]
Samarth Jain commented on PHOENIX-3511: --------------------------------------- [~singamteja], apologies for not being clearer about what that threshold really means. It is basically used by a scheduled thread to check whether the scanner is due for lease renewal or not. So if the time since last renewal is greater than this threshold, then the thread attempts to renew the lease of the scanner. With default value of client scanner timeout, lease renewal will be attempted if the duration since last attempt has been > 0.75 minutes. My question for you would be - have you seen MR jobs fail with client scanner timeout exceptions? If yes, I would probably change the phoenix.scanner.lease.threshold to a lower value like 30 seconds and phoenix.scanner.lease.renew.interval to 15 seconds. This would cause attempts to renew leases to be made more frequently. You don't want to set it too low a value though since every renew lease call is an RPC and making too many of them will prevent HBase from doing the actual work. > Async Secondary index MR job fails for large data > 200 M records > ----------------------------------------------------------------- > > Key: PHOENIX-3511 > URL: https://issues.apache.org/jira/browse/PHOENIX-3511 > Project: Phoenix > Issue Type: Bug > Affects Versions: 4.9.0 > Reporter: Loknath Priyatham Teja Singamsetty > Assignee: Loknath Priyatham Teja Singamsetty > Fix For: 4.9.0, 4.9.1 > > Attachments: phoenix-3511-v2.patch, phoenix-3511.patch > > -- This message was sent by Atlassian JIRA (v6.3.4#6332)


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Julio Oscar Mechoso
31 May 1955 (Age 62)
Julio Oscar Mechoso is an American actor. He is occasionally credited as Julio Mechoso. Mechoso is a character actor in both television and film. He has appeared in several...
Complete: Julio Oscar Mechoso Biography
Famous For
- The Janky Promoters
2010 ( English )
- The Legend Of Zorro
2005 ( English )
- Bad Boys
1995 ( English )
Go to: Filmography
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Hackbright Tech Talk: Hackbright's Nick Avgerinos Discusses Developing Software in Teams
Hackbright Academy
Tuesday, April 29, 2014 from 6:00 PM to 7:30 PM (PDT)
Who's Going
San Francisco, California
London, United Kingdom
Florence, Italy
Event Details
Developing Software in Teams
- by Nick Avgerinos
Hackbright instructor Nick Avgerinos will be speaking about developing software in a team and the phases of a software project at Hackbright Academy on Tuesday, April 29, 2014.
Light appetizers will be served at 6pm, and the tech talk begins at 6:30pm PST.
We will be LIVESTREAMING the tech talk for those who cannot attend in San Francisco - you can sign up to attend the tech talk virtually, or at Hackbright Academy in San Francisco!
About the Talk: Developing software while working with a team can be more challenging compared to when you're just working on a project by yourself. You'll be working with other teams, developers and non-developers, etc. There will also be meetings (lots of those), which usually means there will be some process involved.
This talk focuses on the various phases a software project will go through, from conception to release, and the who and what is involved at each step. We will review popular Software Development Methodologies that you may see out there (Waterfall, Agile, Scrum, Kanban), discuss their similarities and differences, strengths and weaknesses. This talk will give you an idea of what type of development environment best fits your personal style, to help you better know if a company you're interviewing with would be a good fit for you.
This talk is targeted for people new to coding or the industry and focuses on what it means to be a "developer".
About the Speaker: Nick Avgerinos is an instructor at Hackbright Academy. He's been developing software professionally for over 15 years. Prior to Hackbright, he was a Director of Engineering at a gaming company, a systems engineer with Adobe and worked various companies in the VoIP, Wifi and ISP space.
When & Where
Hackbright Academy
683 Sutter Street
San Francisco,
Tuesday, April 29, 2014 from 6:00 PM to 7:30 PM (PDT)
Add to my calendar
Hackbright Academy
Hackbright Academy is a software engineering school for women located in San Francisco, California.
Since 2012, Hackbright has been producing top tier software engineers. We offer an in-person, 12-week, 500-hour, accelerated software development training program exclusively for women to learn Python, HTML, CSS, JavaScript, OOP, TDD, pair programming, Git and the Flask web framework, to job-ready skills and "hit the ground running" as Full Stack Web Development Software Engineers Level 1+.
In addition to engineering fellowships, Hackbright offers part-time courses for product managers, designers, project managers and beginners.
Check out our part-time evening programming courses at Hackbright Academy.


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Помогите нам: эти порнозвезды присутствуют в этом видео?
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Family Law
Family Law Questions? Ask a Family Lawyer Online.
If an estate is opened, the money in the bank goes into the estate. As does all her property.
Then the administrator or executor of the estate can advance some inheritance, if there will be enough money to cover debts, carrying costs of selling the home, etc...


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Each month, Rated People connects over 50,000 homeowners with quality tradesmen - simply enter your job details in the form above to get started.
We'll match your job to the relevant tradesmen in your area - those interested will pay to see your contact details and call you to quote.
As each tradesman expresses interest in quoting on your job, we'll send you their profile and ratings to help you choose the right one for you.
After the work is complete, return to the site and rate your tradesman based on their quality of work, reliability and value for money.
Added 20/01/2017
Services required: Slate / Tiled roof repair Repair: missing / broken tiles/slates, roof ridge,...
Searching for tradespeople
Heating Engineer
Added 05/11/2015
To inspect and test 1 x cooker, 1 x gas fire and a water heater to ensure that they are safe...
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Service gas appliance and issue safety certificate 1 x boiler, 1 x cooker, 1 x gas fire Make...
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quote for veranda with gate and narrow gap in between spindles as i have grandchildren and...
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Managing your job is easier with the Rated People Homes App, download it today.


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Happy 35th Birthday, Gaming PC
December 23, 2014 - How a home computer from 1979 pushed electronic entertainment to the bleeding edge.
SEGA 3D Classic Series Headed to 3DS
July 18, 2013 - SEGA will release eight classic SEGA titles enhanced in 3D for the Nintendo 3DS, including Ecco, Sonic, Shinobi and more.
Space Harrier Retrospective
September 5, 2008 - "Get ready!" Those two words launched eager gamers into the Fantasy Zone, a day-glo universe inhabited by dragons, giant robots, and Easter Island heads. The mission? Players zoom through wave after wave of these colorful nasties while clutching a cannon capable of bringing down even the biggest one-eyed woolly mammoths. The hero of this world is so powerful, he can even fly through the air just by lifting his feet off the ground and willing it to be. If that sounds like a crazy premise to you, then SEGA's Yu Suzuki, head of its famous AM2 division, did his job well.
Sega Ages 2500: Space Harrier
June 9, 2004 - Super Scaler Technology was born of Hang On and put to excellent use in such titles as Outrun, Afterburner and Space Harrier. All these titles were the early work of Yu Suzuki now head of... well, now a guy at Sega (or maybe Sammy) who makes games. Anyhow, starting in 1985, many years prior to the Model boards and their polygons and texture mapping, Sega used big scaling sprites to give off a sense of 3D visuals like never before.
SEGA Ages Compilations in U.S.
May 27, 2004 - While it's common knowledge that Conspiracy Entertainment acquired the rights to several of the SEGA Ages remakes sometime last year, the status of those games has been up in the air for months. Expected by many to be declined by the Sony Approval Board if they attempted to be released individually, the titles in question were often speculated to be collected into some kind of compilation set should they ever hope to see the light of day on our side of the ocean. True to form, it appears that that prospect has finally happened.
Sega Ages Website Opens
June 17, 2003 - 3D Ages, the company formed by Sega and D3 Publisher to release titles in the Sega Ages series of classic remakes to the Japanese market has opened up a website.
Classic Sega Titles Remade on PS2
December 3, 2002 - At a press conference in Tokyo today, Sega and D3 Publisher announced the Sega Ages series of budget titles for the Japanese market. Set to be published by the newly established 3D-Ages company (given birth in October by Sega and D3), this series brings classic Sega titles to new life on next generation consoles at 2500 yen a piece.
September 30, 2002 - Sega of Japan today announced the formation of a new joint venture with D3 Publisher, the Japanese company responsible for the popular Simple 1500 and Simple 2000 series of budget software for PlayStation and PlayStation 2. The new company, owned in nearly equal parts by the Sega and D3P (although Sega holds a 51% controlling interest) plans to re-release games in the existing Sega software library as low-price titles for PlayStation 2, and release some of D3P's Simple 2000 games overseas (i.e., in the United States and Europe).


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Feel free to write up articles once you have signed up but it has to checked by moderators before it can be considered canon in this timeline. Before the timeline has been checked it should be labelled "proposal".
This all events and articles should be plausible and logical based on facts and logical conclusions. Please do not insert ASB elements such as aliens, magic, time travel, time-travelling magic space aliens etc. Unlikely events are okay just as long as they are realistic and are justified.
Time Period-
This ATL starts from February 15, 1933, and continues on until present day. Please keep pace with other contributors and collaborate to make international events instead of rushing ahead and leaving the rest left behind.


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Canvas Prints and Perspex Art
Because it looks better than your usual canvas prints and the colours are fab.
This is the prettiest, pinkest, most adorable chandelier I've ever seen.... I NEED THIS!Added 7 Years Ago from Thatvintageshop
This is the hottest new light up centerpiece item in the special events market. Just introduced, these light up mini contemporary chandeliers make the perfect accessory to any special event or wedding reception.Added 7 Years Ago from Koyalwholesale
This beautiful crystal chandelier is the perfect addition to any princess room!Added 7 Years Ago from Laylagrayce
It's so girlie and cute.Added 8 Years Ago from Csnlighting
Fanstastic print on canvas or perspex print.Added 9 Years Ago from Fourblankwalls
Cute and fully made from recycled materials!Added 9 Years Ago from Straydogdesigns
So, you know how most chandilers have the same shape...well at least in general? I've never seen a starburst chandelier before. And this chandelier looks like an explosion of roses. I think it's kind of amazingly crazy awesome... So, you know how most chandilers have the same shape...well at least in general? I've never seen a starburst chandelier before. And this chandelier looks like an explosion of roses. I think it's kind of amazingly crazy awesome.
Not just for use in the baby's room. Use on any chandelier.
Monogram everything in the nursery!Tags: home, baby, lighting, toddler, embroidery, pink, decor, chandeliers, monogramsAdded 9 Years Ago from Rhbabyandchild
This chandelier will add some serious eccentricity to your dining or living room.Tags: home, lighting, pink, decor, chandeliers, red, Gothic, New Orleans, purpleAdded 9 Years Ago from Neiman Marcus Online
This light is stunning. Absolutely stunning. Missoni Home introduces the Pendant Light - a modern hanging light in a signature, bold floral Missoni pattern. An elegant, artistic touch to any room.Added 9 Years Ago from Neiman Marcus Online
Retro style cylinder shade with bead detail. Suitable for use on table lamps or pendants. 12" diameterTags: home, lighting, lamps, home decor, pink, chandeliers, black, air, dining roomsAdded 10 Years Ago from Thecontemporaryhome