text:
Converting the domain to polyhedra problem?
Hi there,
I am a new Ansys user...I generated the mesh (about 6,000,000 of elements) with ICEM and I eventually exported it to FLUENT...Then I tried to convert the domain to polyhedra however my laptop grot frozen meanwhile the polyhedra cells are being built (says after one hour and an half).
Do I have generated meshes too coarse (min orthogonal quality: 0.32 and medium quality: 0.71) or there may be another reason? Is there anything I can do to improve the process? ( I already set the parallel modality)
Also: in a prevows attempt I get that I did not set the polyhedra feature angle, but how can I actually set it?
Many thanks
you build 6.0 Mil mesh?!?!?!?!?
That's good and do not need to wonder why it is so slow.... Everything is OK!
Fluent has a good feature - if it can not handle the process it complains... so, you just need to wait.... if the RAM is not enough then Fluent will tell you this. Thy to reduce the mesh if it is possible.
|All times are GMT -4. The time now is 11:01.


text:
TypingMaster 10.1.1.849 + portable | 21.3 Mb
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subset: commoncrawl


text: Sneak peek at a freelance piece. Hard truth: I do not drink coffee. Never developed a taste for it. Why drink coffee when you can drink milk? I know, I know, I'm missing out. Sometimes people find out this information about me and are AGHAST. But I do like donuts. Lucy Knisley January 17, 2013


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The bookseller also sells a Nook e-reader device but has always focused on making its collection of 1 million e-books available on other readers and computer platforms, a strategy not followed by all its competitors. The company said today it plans to continue to add more platforms and devices in the future, but didn't provide details.
For the iPhone, Barnes & Noble said it designed the Nook software to maximize e-book cover art and text to take advantage of the iPhone 4's high resolution retina display. The software also works with earlier iPhone and iPod touch models, however.
Nook software is unique in that it allows customers to share certain eligible e-books with other Nook software users for up to 14 days. Nook software is enabled for desktops and Android smartphones as well as iPads and now iPod touch and iPhone devices.
The update to the earlier-released iPad app provides a "rate" feature from one to five stars to let users sort their favorite books.
Matt Hamblen covers mobile and wireless, smartphones and other handhelds, and wireless networking for Computerworld. Follow Matt on Twitter at @matthamblen or subscribe to Matt's RSS feed . His e-mail address is email@example.com .
Read more about smartphones in Computerworld's Smartphones Topic Center.
This story, "Nook e-reader software ready for iPhone, iPod touch" was originally published by Computerworld.


text:
Melvin Seals - Hammond B-3 Organ, keyboards
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Taped and transferred by Andrew Kolodjeski
Tracked, flac'ed and tagged by Alex Ford.


text:
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VICTOR Gollancz, the firm which in its heyday published George Orwell and Kingsley Amis and helped to form the political climate, is ceasing to publish general interest titles.
The decision by Gollancz's owner, Orion, to restrict the imprint to science-fiction titles means the end of a maverick tradition. Sean Magee, a publishing director at Politico's, and a former Gollancz editor, said: "It's a very sad event in the history of publishing. It was one of the great pioneering imprints of the century."
The firm was an independent house from 1928, when it was founded by Victor Gollancz, until 1989 when it was bought by an American publishing firm. It was later taken over by Cassell, and then by Orion in 1998. No one from the group was available for comment yesterday.
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oon after he started his publishing house he began the experiment of issuing big books at low prices on current subjects by authors such as Harold Laski. During the Thirties he found a public of about 50,000 wanting serious books on politics, economics and social affairs for five or six shillings a time.
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It had also managed to keep in touch with its political past, recently publishing Michael Foot's Dr Strangelove, I presume.


text:
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text:
If you got a question, look no further.
We post the most common questions
in our FAQ section.
Please fill in the form below to receive a Free Sample of the Report.
SummaryDawson Geophysical, Inc (Dawson), formerly TGC Industries, Inc is a geophysical consulting company that provides seismic solutions. The company offers acquisition total package and processing services. Its acquisition total package services include planning and design; project management, permitting, surveying, field operations and support services. The company offers processing services such as providing expertise of unequaled depth and breadth of land; and providing processing centers. It operates through its offices and processing centers in Calgary, Denver, Pittsburgh, Denver, Oklahoma City, Midland, Plano and Houston. Dawson is headquartered in Midland, Texas, the US.Dawson Geophysical Company Key Recent DevelopmentsMay 18, 2015: Dawson Geophysical Reports First Quarter 2015 ResultsNov 26, 2013: TGC Industries to Present at the Cowen and Company Third Annual Ultimate Energy ConferenceOct 25, 2013: TGC Industries Announces Third Quarter 2013 Earnings Release and Conference Call ScheduleOct 07, 2013: TGC Industries Announces Third Quarter 2013 Earnings Release and Conference Call ScheduleOct 01, 2013: TGC Industries to Present at the Westlake Securities New Frontiers in Energy ConferenceThis comprehensive SWOT profile of Dawson Geophysical Company provides you with an in-depth strategic analysis of the company's businesses and operations. The profile will give you a clear and an unbiased view of the company's key strengths and weaknesses and the potential opportunities and threats. The profile helps you formulate strategies that augment your business by enabling you to understand your partners, customers and competitors better.This company report forms part of the 'Profile on Demand' Service, covering over 50,000 of the world's leading companies. Once purchased, We will comprehensively research and author a full strategic analysis of Dawson Geophysical Company and deliver this direct to you in pdf format within two business days (excluding weekends).The profile contains critical company information including*,
- Business description – A detailed description of the company's operations and business divisions.
- Corporate strategy – Analyst's summarization of the company's business strategy.
- SWOT Analysis – A detailed analysis of the company's strengths, weakness, opportunities and threats.
- Company history – Progression of key events associated with the company.
- Major products and services – A list of major products, services and brands of the company.
- Key competitors – A list of key competitors to the company.
- Key employees – A list of the key executives of the company.
- Executive biographies – A brief summary of the executives' employment history.
- Key operational heads – A list of personnel heading key departments/functions.
- Important locations and subsidiaries – A list and contact details of key locations and subsidiaries of the company.
Note*: Some sections may be missing if data is unavailable for the company.Key benefits of buying this profile include,
You get detailed information about the company and its operations to identify potential customers and suppliers.
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Table of Contents
Section 1 - About the Company
Dawson Geophysical Company - Key Information
Dawson Geophysical Company - Overview
Dawson Geophysical Company - Key Employees
Dawson Geophysical Company - Key Employee Biographies
Dawson Geophysical Company - Key Operational Heads
Dawson Geophysical Company - Major Products and Services
Dawson Geophysical Company - History
Dawson Geophysical Company - Company Statement
Dawson Geophysical Company - Locations And Subsidiaries
Head Office
Other Locations & Subsidiaries
Section 2 - Company Analysis
Dawson Geophysical Company - Business Description
Dawson Geophysical Company - Corporate Strategy
Dawson Geophysical Company - SWOT Analysis
SWOT Analysis - Overview
Dawson Geophysical Company - Strengths
Dawson Geophysical Company - Weaknesses
Dawson Geophysical Company - Opportunities
Dawson Geophysical Company - Threats
Dawson Geophysical Company - Key Competitors
Section 3 - Appendix
About GlobalData
Contact Us
Note*: Some sections may be missing if data is unavailable for the company List of Tables
Dawson Geophysical Company, Key Information
Dawson Geophysical Company, Major Products and Services
Dawson Geophysical Company, History
Dawson Geophysical Company, Key Employees
Dawson Geophysical Company, Key Employee Biographies
Dawson Geophysical Company, Key Operational Heads
Dawson Geophysical Company, Other Locations
Dawson Geophysical Company, Subsidiaries
Dawson Geophysical Company, Key Competitors
Dawson Geophysical Company, SWOT Analysis
Currency Codes


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Judicial Protection: Unjust Enrichment
The Ninth Arbitrazh Court of Appeals set aside the decision of the Arbitrazh Court of Moscow to recover from the Firm's client over 15 mln rubles for unjust enrichment. The Court of Appeals accepted the arguments of the appeal that prior to state registration of the new owner's (subsidiary's) title to real property no money is subject to recovery for unjust enrichment in connection with the use of such property by the parent company. The appeal was prepared and the client was represented at the Court of Appeals by attorney Marina Abramova.


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|Flange Outer Diameter:
|1.78 in
|Installation Depth:
|0.984 in
|Overall Width:
|0.669 in
|Shaft Diameter Maximum:
|Shaft Diameter Minimum:
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Q:Would it not be advantageous to include the shaft size in your description??? It would then be easier to determine that the redi sleeve your ordering is right. It's written on the end of the box, but no where in your description of the part, so its bought, shipped and recieved before your really sure.(That it is the wrong one)Why is P#99177 listed with '87 Toyota differential parts, it does not match any shaft in the differential?
1 answer
A:This specification of this part National Redi-Sleeve, part number 99147 manufactured by National: Shaft Size Minimum: 1.49", Shaft Size Maximum: 1.496", Width: 0.512", Overall Width: 0.669", Flange Outer Diameter: 1.78", Installation Depth: 0.984".
Q:is this the peace tht goes on the drive shaft where it goes into the transmission
1 answer
A:Yes, this is the seal that goes on drive shaft.
Q:i bought a redi-sleave for my 1994 geo metro 1 liter 3 cylinder auto transmission. I bought the front transmission torque converter seal and a redi sleave for it. your site shows several different part numbers for the redi-sleave and i wanted to make sure i got the right one. the chat person could not help. the part that i got was # 99147 redi-sleave. is this the right part for my FRONT transmission torque converter seal? the lady said this was the output seal, i am unsure if it is input or output. can you help to make sure this is the right part before i put it back together? i have the front seal part number 223830, is this redi-sleave part number 99147 the right part for my FRONT transmission torque converter seal? thanks
1 answer
A:No, this part does not fit your 1994 Geo Metro.
Q:Is this the sleeve for the front crankshaft oil seal?
1 answer
A:This is a differential seal sleeve.
Q:Is this part the whole axle or just the sleeve?
1 answer
A:This is just the sleeve.


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Removal & Installation
- Before servicing the vehicle, refer to the Precautions section.
Remove or disconnect the following:
Negative battery cable
Push in the outer end of the halfshaft to disengage the splines from the hub splines.
Tie rod end nut from the steering knuckle by holding the tie rod while loosening the nut, then use tool C-3894-A to separate the tie rod from the knuckle
Tip the knuckle outward at the top and remove the halfshaft from the hub and suspend the halfshaft using wire.
Ball joint nut using an impact gun, then reinstall the nut until the top of the nut is even with the stud. This will prevent the stud from distorting while performing the next step.
Place removal tool C-4150A over the ball joint and stud and tighten the tool to release the stud. It may help to rotate the knuckle around so the inside of the knuckle faces outwards, then remove the tool.
To install:
When installing the lower control arm-to-cradle rear mounting bolts, make sure the flag on the left control arm nut are positioned upwards (above the control arm) and the right control arm nut are positioned downwards (below the control arm).
Install or connect the following:
New lower control arm and the control arm to cradle bolts from the rear as illustrated and hand tighten
Clean the ball joint stud and knuckle contact surfaces of grease and dirt to avoid damage.
WARNINGThe steering knuckle-to-strut assembly bolts are serrated and must not be turned during installation.. Also if the vehicle is equipped with eccentric strut assembly bolts, the eccentric bolt must be installed in the bottom (slotted) hole on the strut bract.
NOTEThe strut-to-steering are installed differently on each side. The left hand side bolts are installed from the vehicle rear to front and the right side bolts are installed from the vehicle front to rear.
Steering knuckle on the ball stud
- Position the steering knuckle neck into the strut assembly. Install the strut assembly-to-steering knuckle bolts. Install the nuts onto the attaching bolts and torque to 60 ft. lbs. (81 Nm), then tighten an additional 1 / 4 or 90 degrees turn.
Clean the tie rod end and knuckle contact areas to prevent damage.
Install or connect the following:
Tie rod end to the knuckle and hand tighten the nut. While using a socket to hold the stud, tighten the nut to 35 ft. lbs. (47 Nm) plus an additional 180 degree ( 1 / 2 turn).
Have an assistant apply the brakes, install the washer, and hub nut. Tighten the nut to 180 ft. lbs. (244 Nm).
Spring washer, new hub nut and cotter pin
- Check and adjust the brake fluid and brakes.
- Align the vehicle.


text: For those who first encounters with the word "upgrade» («upgrade») explained that this word means rich update, resource and capacity, property, modernization. All this fully applies to your old bathroom. It is perfectly possible to do it with a new and contemporary. How? And this is, how to do alterations? First of all, we enjoy to decide for yourself the question, what will be the maintenance. Simple whitewashed walls and roof of the issues is virtually, It is possible to hold them without involving outside forces, and even reading the article. A some tricky if you decide to substitute plumbing fittings and tile cladding. Even more complicated when you want to enlarge or cut the lavatory, change the placing of its walls, that is, to create alterations to the premises. This is a complex and time-consuming task. Even the bathroom it will demand big financial costs and red tape. First of all, you wish a project of redevelopment. Once it demanded only if redevelopment included alone substitute the bearing wall. The rest of the walls can be tuned independently. But now may request to coordinate any redevelopment projects in ZhEKe and the Bureau of Technical Inventory (NBTI). This in turn will want a mass of documents and the building of their long-term agreements at various levels. According to conservative estimates, the person running on different instances, permission to redevelop the apartment you get at most 4-6 months. There is an easier way – to conclude a contract with the progress company to carry out redevelopment. I defy you to issue the necessary permits in a question of days. Money was used. Approximately 2000 UAH. For illegal alterations to the Administrative Code, you, however an individual, be fined in the capacity of 17 to 51 UAH. With officials nice even steeper – already up to 119 UAH. But if the case was limited to just this! Illegally reschedule the placement you will not be able to sell. In identifying illegal alterations It is possible to make or restore the old layout, or arrange it in a proper manner. Bathroom repair maintenance komnatyPri eternal questions arise: who will do the repair, where to buy progress substances and sanitary plant, however much it will cost and as long the rehabilitation will create it. I would like to maintenance done enough qualified professionals. Otherwise improperly connected plumbing may lead to flooding of neighbors. Qualified repair involve: – Removal of obsolete equipment and preparatory work – aqueduct setup wiring systems for hot and cold h2o, and sanitation; – electric and tiling work – leveling and waterproofing of walls – aqueduct connection – carpentry, hitch accessories. We may go even further. Perform non-standard and more complex work. For example, oblitsevat wash tiles forming intricate designs on the wall, use mosaic wall tile seamlessly with sharpening external and internal corners at 45 degrees. Arrange a niche designer, multi-level ceiling, set up additional devices on water conduit plant. But if you want your toilet was the alone in the world of styling and you keep enough money, you can ordering the overhaul of specialized design firm. This type of maintenance fabulously expensive, it uses highly unique styling solutions. What do the renovation to you.


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Dot's Butterscotch Brownies
Recipe by KerfuffleUponWincle
This recipe is from the 1977 edition of Tea-Time at the Masters Cookbook, page 251 -- my family has enjoyed these brownies for almost 30 years! Probably the best blond brownie you will ever eat!
Top Review by NANCIECHIC
I have looked for this recipe for years! My mother made these for us for 50 years. They just came out of the oven and they are delicious! Making additions is limitless...add nuts,chocolate chips, butterscotch chips, white chocolate chips, macadamia nuts, to name a few. This whole site was worth finding this recipe! So easy!
See Discussion:6 Reviews
Ingredients Nutrition
- 1 (16 ounce) box light brown sugar
- 3⁄4 cup butter (equals 1 1/2 sticks butter)
- 2 eggs, beaten
- 2 cups flour
- 2 teaspoons baking powder
- 1⁄2 teaspoon salt
- 1 teaspoon vanilla extract
- 1 cup nuts, chopped
Directions See How It's Made
- Preheat oven to 350 degrees, or 325 degrees if using pyrex or glass.
- Melt brown sugar and butter together. Cool mixture for about 5 minutes.
- Add eggs.
- Mix flour, baking powder, salt, vanilla and nuts together.
- Add to other mixture.
- Mix well.
- Bake in greased 9 x 13 pan at 350 degrees for 30 minutes ~ if using pyrex or glass, reduce temperature to 325 degrees for 30 minutes.
- Top with chocolate frosting, if desired ~ I usually don't!


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Course Info: Human Resources
Human Resources is a very rewarding career path, which allows you to interact with people and organisations in a very special way.
Human Resources is a very rewarding career path, which allows you to interact with people and organisations in a very special way.
For many graduates, the move into HR is often an uninformed and accidental choice. Human Resources is a very rewarding career path, which allows you to interact with people and organisations in a very special way. It draws together a range of skills and gives an insight into business and an understanding of the inner workings of a company that only a select few people can enjoy.
Many graduates have fallen in love with their choice without previously knowing much about it, so imagine how much you can do with a well-informed introduction to the subject!
Our student editor, Alejandra, is here to give a few more details and explain why a course in HR could be the right choice for you.
"You'll have an insight into the organisation and its people in a very privileged way. Both the organisation and the people will trust you with their secrets; how you manage this gift will define you and your success."- The Chartered Institute of Personnel and Development
Roles in HR range from general positions that incorporate a lot of different activities such as office administration and payroll, through to specialist areas such as Talent management and reward, meaning that you will have a hands-on experience of a variety of aspects within a specific company.
Although courses in Human Resources are compulsory components of any business degree, you can look for a higher degree of specialisation by studying a degree on Human Resources Management, either at undergraduate or postgraduate level.
The degree course considers how people, processes and structures inter-relate. It focuses on the crucial part played by individuals and groups in organisation processes, such as decision making, planning and managing groups of people and state-of-the-art business strategies for all organisations.
Human Resources have a pivotal role in times of recession. Currently, business is roaring for HR specialists as companies are concentrating their efforts in getting the best performance possible out of their employees. This is where HR specialists come in - and you could you be one of them. Take a look below and see if any of the following qualities describe you:
Human Resources specialists also have a very important responsibility supporting employees when they are going through tough personal times such as debt, illness, or grief, and when they're facing intense professional challenges, like an international move. If you are genuinely interested in people and want to incorporate personal interaction into your career, HR is the best way to pursue these interests.
As a Human Resources specialist, you will have the opportunity to interact in different areas such as:
Some of the best paid jobs are currently offered in public sector organisations, as they are aiming to develop their employees into a thriving and cost-effective workforce. Follow the link to find out more information about HR career paths.
If you feel this is your 'call of duty' then do not wait too long to check university entry requirements. Remember that if English is not your first language, you will have to take an English proficiency exam (i.e. IELTS or TOEFL) to prove your language skills. To find out more about how to get ready for the exam, read the article about IELTS that we have prepared for you!
Find out more information about funding by following the link to get a list of the scholarships offered to HR students. However, as rocketing tuition fees may discourage some to get into university; many employers are recruiting talented young people by offering to sponsor their degrees, read the following article to find out more.
Aspiring journalist and Cambridge University graduate, Londoner 'by adoption'. Tweeting for @hotcourses_Abrd
'Study in the UK' eBookEnjoy what you've read? We've condensed the above popular topics about studying in the UK into one handy digital book.


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Phaester Osiris, also called the Throneworld (AUDIO: The Ship of a Billion Years) and stylised as Phæster Osiris, (PROSE: The Sands of Time) was the home planet of the Osirans. It was a hot and dusty planet, (PROSE: Legacy) located in the constellation of Orion (PROSE: The Sands of Time) and having one moon, Beta Osiris, (PROSE: Scarab of Death) as well as the energy production facility Amenthes, orbiting it. (COMIC: Old Girl) Signs of the Osirans' civilisation could be found on the planet, such as the especially tall pyramids. (PROSE: The Dark Path)
Circa 5000 BC, Phaester Osiris was ruled by Horus and Sutekh. (TV: Pyramids of Mars) After the Osirian Court slipped its moorings and cut itself off from history, its homeworld was considered a liability, so Sutekh destroyed it. (AUDIO: Ozymandias) Only Horus and seven hundred forty other Osirans survived the destruction of their homeworld. (TV: Pyramids of Mars)
In 3985, the Braxiatel Collection funded an expedition to investigate the planet. Professor Rhukk helped discover the Horun ruins. (PROSE: Legacy)
Though Phaester Osiris was eventually destroyed by Sutekh, it existed as recently as the 40th century. (PROSE: Scarab of Death, PROSE: Legacy)


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Environmental transcriptome analysis reveals physiological differences between biofilm and planktonic modes of life of the iron oxidizing bacteria
Extreme acidic environments are characterized by their high metal content and lack of nutrients (oligotrophy). Macroscopic biofilms and filaments usually grow on the water-air interface or under the stream attached to solid substrates (streamers). In the Río Tinto (Spain), brown filaments develop under the water stream where the Gram-negative iron-oxidizing bacteria
Total RNA extracted from environmental samples was used to determine the composition of the metabolically active members of the microbial community and then to compare the biofilm and planktonic environmental transcriptomes by hybridizing to a genomic microarray of
Our results indicate that the acidophilic filaments are dynamic structures in which different mechanisms for biofilm formation/dispersion are operating. Specific transcriptomic fingerprints can be inferred for both planktonic and sessile cells, having the former a more active TCA cycle, while the mixed acid fermentation process dominate in the latter. The excretion of acetate may play a relevant ecological role as a source of electron donor for heterotrophic Fe3+ reducers like some Alphaproteobacteria,
The prokaryotic diversity of the extremely acidic waters of the Río Tinto (southwestern Spain) has been studied for many years and is well characterized
We previously reported the environmental transcriptomic fingerprint of
Many bacterial species live predominantly in biofilms in both natural and artificial environments
It has been reported that ore bioleaching requires the formation of biofilms as the mode for bacterium-mineral interaction
Physicochemical and microbiological characterization of sampling sites
Biomass was collected from water and filamentous biofilms (Fig.
Table 1
Physico-chemical parameters of the water in the Río Tinto sampling site.
1.8 ± 0.02
1.82 ± 0.07
21 ± 0.5
20.1 ± 0.5
Conductivity (mS Cm-1)
17.63 + 0.09
27.3 ± 0.01
10.45 + 0.05
16.65 ± 0.05
O2 (% sat.)
13.1 ± 3
SO4= (mg L-1)
70200 ± 220
128000 ± 410
Fe tot (mg L-1)
13970 ± 230
19540 ± 2680
Formate, HCOO- (mg L-1)
2.8 (0.06 mM)
Acetate, CH3COO- (mg L-1)
82.68 (1.36 mM)
Other acetate measurements: 3.54 mM on June 2006, or 0.34 mM on July 2008.
ULD, under the limit of detection.
Figure 1
Determining the microbial community composition of the sampling site
Determining the microbial community composition of the sampling site. (A) A picture of the field site in one of the Río Tinto springs showing details of the filamentous biofilms. (B) Estimation of the active microorganisms in the sampling site by using a prokaryotic acidophile microarray (PAM). Histograms show the signal intensities of each sample (biofilm and planktonic cells) after PAM micrarray hybridizations performed by simultaneous two-color labeled total RNA sample incubation (biofilm+planktonic) of each campaign.
We estimated the microbial community composition by two color simultaneous hybridization of total RNA (from biofilm and water, the same used for gene expression studies, see below) to a prokaryotic acidophile microarray (PAM) already tested and validated with this type of samples
We assume that other
Additional file 1
Additional discussion, tables and figures Additional discussion concerning: the upregulation of genes involved in other functions like sugar metabolism, pentose-phosphate pathway, and oligopeptide ABC transporters. Additional Tables from S1 to S3 Additional figures from S1 to S5 Additional references.
Click here for file
Comparing the
Total amplified RNA from plankton and biofilm was fluorescently labeled (Cy3 for plankton and Cy5 for biofilm), mixed and set to hybridize with the genomic
In previous works
Figure 2
Some of the more relevant up-regulated genes were also checked by RT-PCR and qRT-PCR using total amplified RNA from biofilm and planktonic cells from the 2004 campaign. The preferential transcription detected by genome microarray experiments of
Figure 3
Transcriptional up-regulation of a selected set of genes of
Transcriptional up-regulation of a selected set of genes of
Effect of monosaccharides and small organic acids on the growth of
The toxic effect of small organic acids (acetate, formate, etc) and other organic compounds on the growth of acidophile chemolithoautotrophs is well known
Figure 4
Transcriptional evidence for metabolic differences between sessile (biofilm) and swimming (planktonic) modes of life
Transcriptional evidence for metabolic differences between sessile (biofilm) and swimming (planktonic) modes of life. Genes encoding mixed acid fermentation enzymes are up-regulated in the biofilm (
Figure 5
Additional file 2
DNA sequences of the genes from Additional file
Click here for file
Transcriptomic fingerprints in extremely acidophilic biofilms
The analysis of the environmental transcriptomes of the
Genes involved in biofilm production and maintenance
Several small size genes encoding hypothetical proteins were highly up-regulated in the streamers (Fig.
Genes for quorum sensing and motility
We identified a putative
Genes for the synthesis of cell wall structures
Among the highest up-regulated genes was
Genes involved in mixed acid fermentation
Some genes involved in mixed acid fermentation showed up-regulation patterns as determined by microarray hybridization, RT-PCR and q-RT-PCR analysis (Figs.
The high induction of
The oxygen concentration drops drastically in the water column, so most of the submerged streamers are microaerophilic. Under these conditions, the primary fermenters (Fungi, Actinobacteria, some Firmicutes) in the biofilm community can use the structural components of the biofilm (e.g. polysaccharides, hexoses, etc) to produce pyruvate via the Embden-Meyerhof-Parnas pathway. Then, the same or other bacteria (Firmicutes, Alphaproteobacteria,
Figure 6
Extremely acidophilic streamer filaments are dynamic biofilms and a source of small organic acids
Extremely acidophilic streamer filaments are dynamic biofilms and a source of small organic acids. (A) Scheme showing the main functions related to biofilm formation, dispersion and maintenance that have been detected by environmental transcriptomic studies. In addition, two different metabolic states can be inferred: 1) under microaerophilic conditions in the biofilm, cells activate the fermentative pathways with a net production of acetate, and 2) free living cells under aerophilic conditions showed a high active TCA cycle for energetic metabolism.
The transcriptomic fingerprint of
The highest induced gene (more than 20 fold) in planktonic cells was that encoding a NAD+-dependent isocitrate dehydrogenase that we reported previously
Our results indicate that the main mechanisms governing biofilm formation and dispersion are operating in natural extremely acidophilic filamentous biofilms. The identified genes corresponded to others known to be involved in different stages of biofilm formation in other bacteria
The up-regulation of the mixed acid fermentation genes suggests that the acetate detected in the extremely acidic water of the sampling site (up to 3.54 mM in June 2006) may be a consequence of acetic acid production by the acidophilic biofilms (Fig.
Sample collection
Samples used in this study were collected from a permanent spring running under a pile of pyrite-containing rocks accumulated by mining activities. Sampling was performed in October 2004 and 2005, after dry summers and before any autumn rainfall in the area. Biomass from 4 liters of water was recovered by filtration through nitrocellulose membranes (0.22 μm of pore diameter, Millipore Co.) and filters were immediately placed in 5 ml of RNAlater solution (Ambion) according to the manufacturer's protocol. Up to 2 g of filament samples growing in the same sampling site were collected in 10 ml of RNAlater solution. All samples were frozen on dry ice and kept at -20°C until use.
Determination of physicochemical parameters in the sampling sites
The pH, conductivity, salinity, dissolved oxygen and redox potential were measured in situ with a Multi197i multiprobe device (WTW GmbH, Weilheim, Germany). The elemental composition and concentration (Table
Strain and culture conditions
Environmental RNA extraction and amplification
Samples preserved in RNAlater (Ambion) were centrifuged at 10.000 × g for 10 min and washed in acid water (0.1 M sulfuric acid) for subsequent RNA isolation. Total environmental RNA was extracted and amplified through a method based on T7 RNA polymerase linear amplification as described previously
Estimation of biodiversity by using a prokaryotic acidophile microarray
The prokaryotic diversity was determined by a prokaryotic acidophile microarray (PAM) as reported previously
Genomic microarrays, environmental RNA labeling and hybridization
A shotgun genomic DNA library from an environmental isolate of
Scanning and Data Analysis
Hybridized slides were scanned for Cy3 and Cy5 dyes in a GenePix 4100A Scanner and the images were analyzed with Genepix pro 6.0 software (Axon Instruments). The microarray hybridization results were analyzed using AlmaZen system v.2.1 software (Bioalma, Madrid, Spain) and normalized by applying the locally weighted scatter plot smoothing (LOWESS) algorithm
RT-PCR and real time quantitative PCR (qRT-PCR) Analysis
The relative abundances of a set of up-regulated genes were determined in biofilm and planktonic cells by RT-PCR and real-time PCR. Specific primers for the genes of interest were designed (Additional file
DNA Sequencing and Analysis
Sequencing reactions were performed from plasmid minipreps extracted by the automated pipetting system epMotion 5075 VAC (Eppendorf). Sequencing was made using dye terminator cycle sequencing reactions and run in an ABI Prism 3730xl sequencer (Applied Biosystems). Sequences were analyzed and assembled using SeqMan (DNASTAR package software, LASERGENE Madison, WI) and Phred-Phrap-Consed
Authors' contributions
MMP carried out the sampling, RNA, microarray work and analysis, and RT-PCR and qRT-PCR experiments; MJG carried out the sequence annotation and participated in microarray analysis; AA contributed in sequence analysis and in silico metabolic studies; and VP participated in sampling, microarray analysis and wrote the paper. All authors read and approved the final manuscript.
We thank Marina Postigo and Noemí Álvarez for DNA sequencing work at the Sequencing and Bioinformatics Unit at Centro de Astrobiología (CAB), Miriam García-Villadangos for excellent technical work, and Francisco López-de Saro for the critical revision of the manuscript. This work was supported by grant No. ESP2006-08128 from the Spanish Ministerio de Ciencia e Innovación (MICINN).
Microbial community composition and ecology of an acidophilic aquatic environment: the Río Tinto, Spain
Microbial ecology of an extreme acidophilic environment, the Río Tinto
Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidophilic environment, Río Tinto (SW, Spain)
An oligonucleotide prokaryotic acidophile microarray (PAM): its validation and its use to monitor seasonal variations in extreme acidophilic environments with total environmental RNA
Bioleaching review part A: progress in bioleaching: fundamentals and mechanisms of bacterial metal sulfide oxidation
Characteristics and adaptability of iron- and sulfur-oxidizing microorganisms used for the recovery of metals from minerals and their concentrates
Mineral theories of the origin of life and an iron sulfide example
The Río Tinto, an extreme acidophilic environment under control of iron, as an analog of the Terra Meridiani hematite site of Mars
Instrument development to search for biomarkers on Mars: Terrestrial acidophile, iron-powered chemolithoautotrophic communities as model systems
Extreme environments as Mars terrestrial analogs: The Rio Tinto case
Analysis of environmental transcriptomes by DNA microarrays
Microbial biofilms
The biofilm matrix-an immobilized but dynamic microbial environment
Microbial biofilms: from ecology to molecular genetics
Physiological heterogeneity in biofilms
Microorganisms involved in bioleaching and nucleic acid-based molecular methods for their identification and quantification
Extracellular polymeric substances mediate bioleaching/biocorrosion via interfacial processes involving iron(III) ions and acidophilic bacteria
Communication in bacteria: an ecological and evolutionary perspective
Second acyl homoserine lactone production system in the extreme acidophile Acidithiobacillus ferrooxidans
Community proteomics of a natural microbial biofilm
Community structure and metabolism through reconstruction of microbial genomes from the environment
Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by Real-time PCR and oligonucleotide Prokaryotic Acidophile Microarray (PAM)
Differential gene expression of industrial bioleaching transcriptomes by Leptospirillum ferrooxidans DNA Microarray
Amplification of low quantity bacterial RNA for microarray studies: time-course analysis of
Gene function analysis in environmental isolates: The
Effect of sludge dissolved organic matter on oxidation of ferrous iron and sulfur by
Characterization and Functional Analysis of the
Contribution of the SirA regulon to biofilm formation in
BdlA, a chemotaxis regulator essential for biofilm dispersion in
A two-component system involving an HD-GYP domain protein links cell-cell signaling to pathogenicity gene expression in
Biofilm dispersal in
Autoinducer 2 controls biofilm formation in
Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coli
Escherichia coli toxin/antitoxin pair MqsR/MqsA regulate toxin CspD
Depletion of apolipoprotein N-acyltransferase causes mislocalization of outer membrane lipoproteins in
Effects of lipoprotein biogenesis mutations on flagellar assembly in
Structure and function of the Mur enzymes: development of novel inhibitors
Evidence that acetyl phosphate functions as a global signal during biofilm development
The Pta-AckA pathway controlling acetyl phosphate levels and the phosphorylation state of the DegU orphan response regulator both play a role in regulating
Extraction of extracellular polymeric substances from extreme acidophilic microbial biofilms
Effect of sludge dissolved organic matter on oxidation of ferrous iron and sulfur by
Toxicity of select organic acids to the slightly thermophilic acidophile
Production of glycolic acid by chemolithotrophic iron- and sulfur-oxidizing bacteria and its role in delineating and sustaining acidophilic sulfide mineral-oxidizing consortia
Community genomic and proteomic analyses of chemoautotrophic iron-oxidizing "Leptospirillum rubarum" (Group II) and "Leptospirillum ferrodiazotrophum" (Group III) bacteria in acid mine drainage biofilms
Novel insights into the role of potassium for osmoregulation in
Structurally diverse natural products that cause potassium leakage trigger multicellularity in
Potassium and sodium transporters of
Transcriptional analysis of the
Removal of phosphates and fluorides from industrial wastewater
Depletion of intestinal phosphate after operative injury activates the virulence of P aeruginosa causing lethal gut-derived sepsis
Temporal gene-expression in
Solvent extraction and spectrophotometric determination of iron (II) with di-2-pyridyl ketone bonzoylhydrazone
Nitrogen fixation by
Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation
High throughput real-time quantitative reverse transcription PCR
Viewing and Editing Assembled Sequences Using Consed
Improved microbial gene identification with GLIMMER


text:
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Defending life from the moment of conception
SPUC supports parents and medical professionals in helping to protect children, before and after birth
Read about our founders, the aims spelt out in our constitution and some of the campaigns we've been involved in
We stand shoulder to shoulder with the African pro-life movement as part of the global witness to life
SPUC has been defending unborn children for nearly 50 years, both in the UK and internationally
We want you to be a part of our life-saving work: get in touch to find out how you can help end abortion
The universal right to life of every human being is the foundation on which all other rights rely
Pro-life feminists represent a growing number of women who believe passionately in women's empowerment and the universal right to life
Want to find out more about abortion? Wondering about the hard cases? Our FAQ page is here to answer all your questions
Be the first to know about breaking pro-life news stories
Read our latest blogposts from famous pro-lifers, local activists, SPUC staff, guest stars and more
SPUC's charities bulletin is a guide to where many well-known charities stand on abortion and other pro-life issues
17 January 2012
Every year since 2008 SPUC has hosted the International Youth Pro-Life Conference.
Around 150 delegates join us from colleges and universities from around the world. This year's the conference is on 2-4 March and the theme is 'Human Dignity'. We have a fantastic line-up of speakers who will address a wide variety of pro-life issues.
For more information, see "Young people are emerging on the front line of the pro-life movement" [John Smeaton, 16 January]


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Nyack Vacation Packages
Some 7,000 people call Nyack in Hudson Valley home.
Albany, the state capital, is situated about 110 miles (177 kilometers) north of Nyack, while New York is 25 miles (40 kilometers) to the south.
Hit the trail and enjoy the popular natural preserves for which this area is famous. Harriman State Park, Rockland Lake State Park and Tallman Mountain State Park are among the more popular of these. All these parks have their own unique wildlife and plant life.
Just because you're traveling don't neglect your obsession; Nyack is home to a number of the fastest greens, nastiest bunkers and friendliest clubhouses in in the region. Grab a buddy or join locals for a few holes at Blue Hill Golf Course or Patriot Hills Golf Club. If you're in the region for a while, check out the greens at the Broadacres Golf Course.
Take a break from the ordinary for a while and take the kids into a realm of creativity and excitement at Hudson Highlands Cruises and Lower Hudson Valley Challenger Learning Center. Don't miss these two fun and often educational attractions, offering thrills and laughter for children big and small.
Nyack is a great place for art lovers so when you are in the area, make the time in your itinerary to visit the Rockland Center for the Arts and the Edward Hopper House Art Center. These are excellent places visitors can pay a visit to for a few hours of inspirational art and collections. Join a tour, admire the interior of the building or simply enjoy the wonderful exhibits in these cultural precincts.
The Holocaust Museum and Study Center is the perfect escape for lovers of learning in Nyack. Admire the building and grounds, lose yourself in the exhibits, then ask around to discover some of the more eclectic museums in town.
One of the more popular historic sites in the area is the Stony Point Battlefield State Historic Site. This atmospheric place offers visitors the opportunity to learn about an important chapter in time. Check out the site, snap some pictures and learn about the backstory that makes it so interesting.
If you enjoy souvenir and fashion shopping, explore the various stores at the Palisades Center Mall. It is a busy area packed with interesting fashion, quirky accessories and grocery shops.


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Become a member of NASW today!JOIN TODAY!
SOLD OUT! Ethics: Who's the Client? - The Ethical Implications… (Chicago)
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LESS THAN 2 SPOTS LEFT! Life Planning for Your Transition: The Role of the Clinician (Chicago)
Become a member of NASW today!JOIN TODAY!
Visit the CEU Opportunities page to find free CEU events for NASW Illinois members!Continuing Edcuation
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For more information about how HBA Consulting can assist you with Performance Management related issues, please contact:
Gary Champion
Principal HBA Consulting Tel : (02) 62474490
Mobile : 0419401250
Performance Management Service
In the Australian Public Service, the Public Service Commissioner's Directions 1999 require agencies to put in place a fair and open performance management system that:
Further to this, the Australian Public Service Bargaining Framework requires all salary movement within a classification and/or broadband be based on at least a satisfactory performance rating.
These requirements exist in the Australian Public Service as it is widely recognised that effective performance management leads to an efficient and productive workforce.
HBA Consulting Performance Management Services can provide your organisation with economical, expert and pragmatic assistance in:


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28 - 29 May 2008,
New York
Price realised USD 67,000
Luis Tomasello (Argentinian b. 1915)
Reflexión No. 75
titled, dated and signed 'REFLEXION No. 75, 1961 Paris, L. TOMASELLO' (on the reverse)
painted wood construction on masonite
29 5/8 x 29 5/8 in. (75 x 75 cm.)
Executed in 1961.
New York +1 212 636 2000
London +44 (0)20 7839 9060
Hong Kong +852 2760 1766
Shanghai +86 21 6355 1766
Born in La Plata, a suburb of Buenos Aires, the young Luis Tomasello worked alongside his father in construction picking up skills that would both influence him and enable him to assemble his complex and magical constructions of the years to follow. His work during the 1950s exhibits an early interest in a geometric aesthetic but is predominantly figurative. The artist journeyed to Europe for the first time in 1951 and upon his return home met both Emilio Petorutti and Carmelo Arden Quin; the latter, had along with Edgar Bayley, Rhod Rothfuss, Gyula Kosice, Tomas Maldonado and Lidy Prati, published the only issue of Arturo magazine in 1944 considered the founding moment in the development of Concrete Art in Argentina. The movement sought to strip painting from the burden of figurative representation--its belligerent manifesto expressed a new art for a new age and the idea of pure invention and the freeing of the picture plane once and for all.(1)
The artist's trip to Paris in 1957 marked profoundly the way he saw and understood space, movement, and above all--light. Kazimir Malevich and Piet Mondrian became sources of inspiration in his search for the essence of reality--a new way to capture not the fleeting but the poetic quintessence he sought in art. Utilizing the cube as his most versatile ingredient throughout his life, Tomasello would create a way to paint with light and shadow solely rejecting, in theory and material, the painted image and the instruments that made it possible. In Paris, his work was shown at Denise René Gallery alongside the work of Jean Arp, Victor Vasarely and Jesús Rafael Soto; he also became part of the Kinetic Art movement there.
Tomasello's work predominantly is the exploration of light and its effects through the repetition of the cube on the surface of the square or structures he constructs. Through various placements of the geometric shape, he creates tension and movement that is at once poetic as it is an optical illusion.
1) Fundación Proa, Argentine Abstract Art, Buenos Aires, 2003, 12.
Galerie Denise René, Paris.
Acquired from the above by the present owner.
From Yves Saint Laurent in Paris to David Hockney in New York, the must-see shows for the second half of 2017
Exceptional pieces offered in the second half of 2017 in Paris, New York and Geneva


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Dalsouple rubber flooring at the new Royal Childrens' Hospital
Dalsouple DalNaturel rubber flooring has been installed at the newly opened building at the Royal Children's' Hospital in Melbourne. The designers at Bates Smart created unusual 'boulder' shapes out of the rubber tiles in between the ceramic tiles.
The Queen officially opened the new building on her visit to Australia. The opening ceremony was held near the entrance and many officials would have sat or walked over the Dalsouple tiles.
The Terrazzo (speckled) design is unique with an apple green background, white, grey and black speckles on an R10 slip rated texture.
Dalsouple can make up flooring in almost any colour imaginable, with no minimums and no extra cost for special colours. All colours are available in over 30 textures, including walling, rubber skirting, and stair nosing. The DalNaturel formulation is made with 90% natural latex rubber and natural clay particles. This product can contribute to Green star points on Green star projects.
For the first time in 15 years, Dalsouple Australasia is now buying Dalsouple directly from the factory in France rather than through the UK. This is saving on lead times and freight costs. Small orders only take 2 weeks to manufacture and then landed in Australia within about a week with Qantas Couriers. They can ship larger orders In 6 weeks. The factory has a 123 page stock list, with most colours in smooth available up to about 60m2, and some textures in small quantities.
We import natural, synthetic, and recycled rubber flooring for domestic or commercial projects.Learn more


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Complete the Picture. Document your life story or a part of it that you want to have remembered as your legacy. Is there a family business with some great stories or an organization you were deeply involved in?
"I always enjoy working with Connie. The flexibility, attention to detail, and can do attitude is much appreciated in each and every event we produce together. Connie's involvement in community events and nonprofits speaks volumes regarding her commitment to making the world a better place each day."
-- Steve DeAngelo, President at DeAngelo's Catering & Events


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Report summaryThe Yamburgskoye field lies in Yamal-Nenets Autonomous Okrug in northern West Siberia. It is one of Gazprom's 'Big Three' gas fields, along with Medvezhye and Urengoiskoye. Together they have provided the bulk of Russian gas over the past few decades. Yamburgskoye is operated by the largest Gazprom's subsidiary in terms of production, Gazprom dobycha Yamburg. This subsidiary also operates the giant Zapolyarnoye field, the smaller Tazovskoye field and oversees the development ...
What's included?
This report includes 2 file(s)
- Yamburgskoye PDF - 939.51 KB 18 Pages, 16 Tables, 8 Figures
- Yamburgskoye XLS - 664.50 KB


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TOSA La Laguna is a wonderful and beautiful place, a serene nature reserve. A great place to disconnect, get away from it all and go within. There is a mystical quality to the lake which is very nourishing and energizing to the mind, body, and soul. The casitas at TOSA LA Laguna are absolutely gorgeous and wonderfully furnished, the beds are very comfortable! The food was just amazing, delicious and truly nourishing. The staff is great and very attentive, they really work hard to accommodate your stay. The lake waters are pristine and a swim in the lake is a healing experience in itself. I recommend TOSA La Laguna to anyone looking for a blissful getaway.
- Official Description (provided by the hotel):
- TOSA LL is a unique boutique eco-resort and private nature reserve on the shore of beautiful Lake Atitlan. Located at the cleanest and deepest part of the lake, the center is 100% eco friendly. Delight in the luxurious custom spa treatments and indulge in holistic health programs. All-inclusive, (3 meals per day plus snacks), with personalized cuisine and attention.Each of the five casitas is a stunning suite with tempur-foam beds and organic amenities. Abundant and unlimited clean drinking water along with personalized attention highlight your experience. While on-site enjoy hiking, spa services and gourmet cuisine or simply relax into the best sunset view of the lake.Discover peaceful tranquility, privacy and the gift of boat only access. Easily access all other parts of the lake for day tours and return to your sanctuary in the evening. TOSA La Laguna is designed to accommodate your comfort and assist you to vision your dreams...relax, rejuvenate and discover the unspoiled beauty. ... more less
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Readers ask me all the time for recommendations for other authors. I can only write so fast and some of you are looking for your next read between my releases. So I've joined forces with four other urban fantasy/paranormal romance authors and we're having a contest.
All you have to do is sign up for each of our newsletters!
What can you win?
Cash. You can win cash. Right here. That's right, CASH!!
Want to know who these amazing authors are?
And finally me!
Sign up for each of their newsletters and dive into new worlds, new romances, and brilliant adventures with each of these talented authors. Ready, Set…..GO!
Entrants must be 18 years of age or older to enter. Contest open internationally.
Winners emails will be checked against the newsletter sign ups. Any shenanigans like signing up AFTER you're told you've won, or not actually signing up, will cause an immediate loss of coolness and prizes. If you are already signed up for an author's newsletter, you may NOT sign up a second time to earn entry.
Contest will run from February 24th -March 6th midnight at EST will be the cut off.
The authors promise not to swamp you with newsletters or email. Newsletters are typically sent out once a month.
done ! cant wait to read these authors
Thank you, looks like I have some more great books to read!
I'm excited to read all these new books! I'll have to tell my husband goodbye for awhile, I'll be in my imagination until further notice! 🙂
Love to have mail from authors or bloggers about books thanks
Must admit Denise Grover Swank is the only one of the above authors that I have read anything by yet. Look forward to seeing what the other authors books are like.
Such an awesome contest! I am signed up for every newsletter but I'm not going to lie…I sign into raffelcopter with facebook which is a different email than my newsletter subscriptions! Oops.
Thank you all so much for this amazing giveaway ♥
You've made some good points there. I looked on the web to find out more about the issue and found most people will go along with your views on this site.
Feel free to visit my web site: my latest blog post


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Welcome to Firefox for Android Add-ons.
Add extra features and styles to make Firefox for Android your own.Close
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Rated 5 out of 5 stars
INCREDIBLE!!! GREAT WORK!!! EXcelente, el mejor
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GRAVY TRAIN - Staircase To The Day [full album] MP3
Artist: Gravy Train Album: "Staircase To The Day" 1974 Genre: Heavy Prog Label: Dawn Records Country: UK 1. Starbright Starlight (0:00) 2. Bring My Life On ...
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Chandigarh: Ex-servicemen will get arrears to the tune of crores of rupees under the 'One Rank-One Pension' (OROP) scheme and this would give a fillip to the economic development of Haryana, Chief Minister Manohar Lal Khattar said today.
Manohar Lal Khattar. File pic
"It is a matter of pride for me that one out of every ten soldiers of the Indian Army belongs to Haryana," he said in a statement here. Khattar said crores of rupees would be received by ex-servicemen under the OROP scheme which would be spent in Haryana in one way or another, leading to the strengthening of the economic condition of the state.
He urged the ex-servicemen, who had retired early or on medical grounds, to beware of "misleading information being spread by certain political parties about the OROP scheme". The matter had been hanging fire for the last 42 years but Prime Minister Narendra Modi had been working towards fulfilling his promise to resolve this issue since he came to power in May 2014.


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LAS VEGAS (AP) When MTV came to Las Vegas three years ago, some of the Strips biggest hotel-casinos
Current subscribers have full access to all online news content as part of their subscription. If you are a current subscriber, please log in with your website account information for full access. If you are a current subscriber, but have not yet registered for access to the website, please call our Circulation Department at (330) 821-1200


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WA Police has a new Deputy Commissioner.
Gary Dreibergs, who has more than 30 years' experience as a police officer, has been appointed to the role.
The appointment is not a complete surprise - Fairfax Media reported that the WA Police Assistant Commissioner was expected to be promoted at the start of December.
Mr Dreibergs will take up the role as one of two deputy commissioners, joining Stephen Brown.
Deputy Commissioner Chris Dawson vacated his position earlier this year to become chief executive of the Canberra-based Australian Crime Commission, a national crimefighting agency charged with dismantling serious and organised crime.
He had held the deputy commissioner role with WA Police since 2004, after joining the force as a police cadet in 1976.
Mr Dreibergs joined WA Police in 1983 and has served in several operational roles including in Albany and Broome, as well as several back-of-house positions.
For the past two years, he has overseen regional WA - a policing jurisdiction of more than 2 million square kilometres.
Prior to that, he served as the professional development assistant commissioner with responsibility for training and recruiting.
In his time as assistant commissioner, he has overseen major changes including the introduction of custody and auxiliary officers to the force, which has freed up more police officers for frontline duties, and the implementation of new investigative practices.
Mr Dreibergs was awarded the coveted Australian Police Medal in 2010.


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Very funny!! Oh, and thanks for calling me an "amazing prodigy"
Luv, Boobacat
~The 12- year- old HP obsessed fanfic reader~
Can you say random? No really, that was odd...
hehe! That was really weird! Definitely an original idea! You're really good at describing the right details but at some parts I'd get lost because things are being revealed really quickly. Make sure you don't jump between slow and detailed, to fast-paced too quickly. You construct your sentences really, really well and I like the way you write about the trio esp. Ron! Keep at it!
Author's Response: I'll remember the tip. Thanks!
Author's Response: I'll remember the tip. Thanks!
This story made my brain hurt...but i guess thats my own fault...Dunno how u managed to think of that plot!! anyway good job
That was great, and earned me some funny looks when I laughed out loud! Great job-thanks for the reviews!
i actually thought it was weird and funny at the same time a hilarious title that made me and my friend go bonkers