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Some employees believe all they have to do to invoke FMLA leave protection is to call in, say they're "sick" and wait for their employer to request medical certification.
Wrong! Merely calling in sick doesn't trigger any employer obligations under the FMLA.
To put you on notice of their need for FMLA leave, an employee doesn't have to put his FMLA request in writing or even specifically mention "the FMLA" or "family leave." But he does have to provide more than a statement that he's sick and can't come to work.
Recent case: Reginald Lee had been warned that he might be fired if his attendance problem didn't improve. One day, after using up all his sick and vacation leave, he called in sick. He said he had injured his back changing a tire and wouldn't be in for three days.
Lee returned on the fourth day with a doctor's note explaining that he had been examined, but could now return to work. It said Lee's back pain was "mild," "constant" and "tight." The note said nothing about treatment or medication.
Lee was fired for poor attendance. He sued, alleging he was out on FMLA leave and had officially put his employer on notice when he made the initial phone call.
The court said that wasn't enough. It said that merely calling in doesn't trigger an employer's duty to ask more questions. Plus, nothing in the doctor's note qualified Lee for FMLA leave, since it did not specify that Lee couldn't perform his job for at least 72 hours. (Lee v. U.S. Steel, No. 11-11718, 11th Cir., 2012)
Online resource: For advice on how to correctly collect employees' medical information under the revised FMLA rules, read "What's up, doc? How to collect medical info under new FMLA rules."
Related Articles...
- How to Fire an Employee the Legal Way: 6 Termination Guidelines
- Consider additional leave as ADA accommodation
- Counting paid time off as FMLA leave? Tell employee you're running them concurrently
- What kind of pregnancy and family leave rights do gestational surrogates have in California?
- Time records crucial in FMLA eligibility calculation


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subset: commoncrawl


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ATV, Jeep Adventures – Capitol Reef Country » 1116-JeepTour72
Jeep tours in Capitol Reef Country.
Jeep tours in Capitol Reef Country.
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1-800-858-7951 (toll-free in the US)
1-435-425-3365 (International/Direct)


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Central Valley Science Project
A "Professional Home" for K-16 teachers of science.
Fresno and Central Valley Science
Dear Colleagues,
After many years of effort we are pleased to report progress on gaining approval for high school Earth sciences to qualify for UC admission requirements. A new high school course, entitled Honors Earth Science, has been approved by the UC Academic Senate Board on Admissions and Relations with Schools (BOARS), and also by the UC Office of the President as meeting the UC laboratory science "d" requirement for UC admissions. The course was designed principally by high school science teacher Wendy Van Norden with help from the rest of us. It is listed on https://doorways.ucop.edu/list/, under the listings for Harvard Westlake School, North Hollywood, CA. The course outline is attached to this email. In addition it is on the website at Harvard Westlake School at http://www.hwscience.com/Geology/Honors/ESS%20course%20outline.doc.
The successful strategy has been to design a template for a high school course that can be widely adopted by California high schools and attract UC-bound students.
The effort that led to the approval of this course is not the same as earlier ones (2003-2010) by Eldridge Moores and Bruce Luyendyk. Their earlier proposal was to convince the Academic Senate through BOARS and the Academic Council that UC should add "Earth and Space Sciences" as one of the main sciences eligible for "d" credit, in keeping with the NRC "National Science and Education Standards". The UC Academic Senate rejected that approach in June 2010, and the Senate still holds to the view that the "PCB's"-Physics, Chemistry, and Biology are the "basic sciences". This new approach does not challenge that view, but it has the same goals as our earlier effort: recognizing the Earth sciences as an area worthy of meeting UC entrance requirements, and increasing exposure of UC-bound high school students to the Earth sciences.
BOARS in their review was enthusiastic about this course. In their minutes of June 3, 2011, they stated, "... the course in question is strong and should be a model area "d" course". In addition, "BOARS should support and encourage the development of such courses".
This new course has prerequisites of biology and chemistry, two of the three UC area d "basic sciences", as well as algebra, which is a requirement for "d" status certification. The course covers Earth sciences (including a segment on astronomy) with biology and chemistry (as well as algebra) as foundations. It is a strong advanced course; it will be taught at a higher level than existing 9th grade Earth Science classes that do not have and will not achieve "d" certification.
The Honors Earth Science course is intended for high school juniors and seniors. In principle, any high school that adopts this course should receive UC's "d" Laboratory Science approval for it. Widespread adoption of this course in CA high schools should significantly increase the awareness of the Earth sciences by CA high school graduates, UC's entering students, and the public at large.
There are several advantages for students to take a third science course in high school:
UC's d Laboratory Science requirements states "two and preferably three years of high school laboratory science are required"; Three years are better than two; almost all (~90%) entering first-year UC students have three or even four years of science in high school. Now it is possible for the first time that one of these courses can be in Earth sciences. The course has Honors status, and that gives students an edge in UC admissions and a grade point bonus.
Earth science underlies any understanding of the landscape, agricultural patterns, the location and character of towns and cities, resource and economic issues, and Earth hazards. Especially in California, all people need some background in Earth science in order to become informed citizens because of California's Earth hazards, including earthquakes, landslides, floods, and tsunamis.
In this increasingly crowded world, resource and hazard issues are at the forefront of many events and conditions on Earth, e.g. the Japanese earthquake and tsunami, the Thailand and Pakistan floods, the East African droughts, the climate change crisis. Knowledge of Earth science is essential for every citizen, including those who attend UC!
We hope that this new development will encourage high schools around California to offer this course in advanced Earth science, and thus better prepare their students to enter UC and to function as informed citizens in the 21st century. To do so, however, the high schools will need support (approximately $8-10,000 each) to set up the course, get the equipment, books, etc. We professional Earth scientists can help in this regard if we know of funding sources.
The ultimate achievement of this development would be to attract more entering UC students to take courses in the Earth sciences and to have them consider it as a college major and a career choice.
Ray Ingersoll, UCLA
Bruce Luyendyk, UCSB
Eldridge Moores, UCD
Tom Traeger, La Canada High School, La Canada, CA
Wendy Van Norden, Harvard-Westlake School, North Hollywood, CA
Views: 428
Finally!! Thank you for all your hard work!
That is such good news! I'm spreading the word in Fresno Unified. It is exciting to see Earth Science finally recognized as a lab science course and I hope everyone recognizes its potential and relevance. What can we do to make the course happen and have a few high schools in the valley add it to their master schedule for the 2011-2012 school year?
© 2017 Created by Will Kimbley. Powered by


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I don't need to do homework, because the teacher already knows the answer. He doesn't need me.
Sent from my iPhone
I don't need to do homework, because the teacher already knows the answer. He doesn't need me.
Sent from my iPhone
Another year, another #NC11 Dylan Wiliams workshops and I can not recall a workshop where I didn't leave with more questions than answers. I anticipate that this year will not be an exception.
Dylan has authored two professional development training packs for the SSAT with Siobhan Levy that support teachers in embedding formative assessment into their classrooms.
'Why do we think we have done it, when we have not?'
It would appear that Assessment for Learning is still somewhat misunderstood, or given at least considered a box thats already been ticked. What is more concerning is that current feedback practices are rotting away student progress. 4b is not feedback, its data. Current grade, E, Predicted grade D is not feedback, it's a learning thermostat. It's not informative. Again, as per the last two workshops, the message is clear. Grades or levels is not feedback. Even worse grades negate any effort spent on writing comments.
What always impresses me most about these workshops is the conscious, yet polite way, Wiliams' flips the securities of the room. Instantly reversing the years of experience of the teachers in the room by redefining their role as a student. Clearly, not a comfortable role for quite a few in the room. Wiliams not only communicates his philosophies by models them as well. A confident practitioner in his element.
From here, the post is more notes than a formal post as I really wanted to attend to the workshop.
There is a science to praise. Effective praise 'praises work within the students control, sincere, credible, genuine, (slightly less than average).'
Focusing on improvement requires a different grading process. The plus, equals and minus grading system. Now the feedback experience promotes progress.
Feedback should be forward looking. Comment should be guiding, progressing and
Feedback should be more work for recipient than the donor.
Do you maximise your efforts to offer effective feedback? Ask yourself – in your teaching what proportion of your teaching feedback is acted upon and improved? 10%? 20%? 30%? 50%? Were you concerned by you own response?
Perhaps feedback is only part of the conundrum. Where is the responsibility for success? Attribution is key. The best learners believe it is down to them. Levels are not within a student's control.
Interesting feedback can 'typically have eight outcomes, six are bad.'
This is simple slides emphasises just how challenging providing effective feedback is. It is nearly as difficult as setting the most appropriate activity to activate the learner, whilst ensuring that the learner believes they are in control and smart.
In case you were wondering
Assessment for Learning is the process of seeking and interpreting evidence for use by learners and their teachers to decide where the learners are in their learning, where they need to go and how best to get there (Broadfoot et al., 2002 pp. 2-3)
Assessment for learning is any assessment for which the first priority in its design and practice is to serve the purpose of promoting students' learning. It thus differs from assessment designed primarily to serve the purposes of accountability, or of ranking, or of certifying competence. An assessment activity can help learning if it provides information that teachers and their students can use as feedback in assessing themselves and one another and in modifying the teaching and learning activities in which they are engaged. Such assessment becomes "formative assessment" when the evidence is actually used to adapt the teaching work to meet learning needs. (Black et al., 2004 p. 10)
Feedback should causing thinking.
Given a fairly positive first core day, I was giving 'planning' more consideration that I usually would on a Monday afternoon. Specifically, when and how you learn to plan effectively. So, given the NCSL materials and the commentary from Maggie Swinnerton, this is a document I hope to share and get feedback on, from the colleagues I work with in Curriculum areas. Feel free to add, comment and reuse.
We often plan change with ready made planning steps. Not unlike those presented below (adapted from the NCSL Middle Leaders materials). More recently I have began to consider that we often overlook or anticipate the 'change impact' on you, the change agent.
Most plans fail because they are never tried or are implemented without any thought as to what might go wrong; Consider therefore;
Your vision is not static, evolving with practice. When we change, we learn, how can that change process be included, or at least highlighted, as part of the implementation of the plan.
Clearly define the impact you are aiming to achieve. The evidence you aim to collect and evaluation processes.
The impact on the staff you are working with (both teaching and non-teaching) on students, parents, guardians and carers, the community, the governors.
Note to self; consider and reflect the impact implementing the plan will have on you, the change agent.
The day started early. iO have kindly decided to support Interactive Fiction platform Quest and together with Andy Goff and Alex Warren we are hurriedly drafting an introduction competition for teachers and educator with some fantastic prizes. More of that later…. A short drive to the De Vere Grand Harbour for Core Day 1 'Taking First Steps' of the Leadership Pathways programme. A warm welcome from Maggie Swinnerton, coffee and pastries and of course the usual house keeping notifications. Why 'Leadership Pathways.' So far its been a remote experience, a 360 diagnostic, a brief coaching conversation with our Principal and the first of many online learning units. (Knowing that our Principal is fully supportive and committed to the coaching process). From the NCSL's point of view, the clear emphasis is on being an 'effective self-directed learner' and 'leader.'
An overview of the course, the year on Leadership Pathways, and conversation around the school impact study followed. Listening to our Primary colleagues, the size of the school, the infrastructure would appear more readily positioned to promote whole school impact, from 'International Partnerships,' to numerous whole-school subject focused programmes, eg literacy and numeracy. Our Secondary colleagues appear to be more hemmed in by their subject commitments and the perception of the size of the task – in particular the perception of the size of the task in secondary schools.
After a short coffee break, we were back to the task of defining the conditions for effective self-directed learning.
This list led to an introduction to Boyatzis – Intentional Change Theory, a critical segway to uncovering one's motivations for this process or course.
Intentional change is hard work and often fails because of lack of sufficient drive and the proper intrinsic motivation for it. This model of the Ideal self creates a comprehensive context within which a person (or at other fractals, a group or system) can formulate why they want to adapt, evolve, or maintain their current desired state.
This was the broker to the first set of philosophical, leadership questions as we attempt to define our moral purpose.
Interestingly, my 'who' is first and foremost role of husband, father and friend. That aside, in the workplace I aim to demonstrate
As a teacher – to challenge and inspire young people, to role model a love of learning, to encourage and value risk-taking and the resilience to manage failure, to demonstrate a level of consistency that enables students to accurately describe me
As a leader – to demonstrate integrity, decisiveness, an analytical ability, approachable, imaginative, a willingness to give of oneself, an ability to face adversity, to evolve from a 'doer' to an 'empowerer', to demonstrate a level of consistency that enables staff to accurately describe me (a might need to manage the order, and accept that the order will change.)
That's sufficient self reflection for now thought the importance of that task is to measure the distance between my ideal self and my real self?


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|Guild Information
|Guild name
|Invictus Letum
|Server Type
| PvP
General Information Edit
- Guild Name: Invictus Letum (Disbanded)
- Guild Website: N/A
- Guild Forums: N/A
- Main Time Zone: EST/CST
- Guild Leader:
- Guild Recruiter:
- Guild category (PvE, PvP, Raid, Other): PvP/PvE
- Roleplay:
- Voice: Ventrilo
- Recruiting members status: Dorment
- Server type: PvP
- Platform: PC
- Age: All possible members that are under 18 must be interviewed on Ventrilo
About us Edit
Invictus Letum (IL) is a guild composition of like minded individuals that work together in game to make it fun, rewarding and a nice place to call home.
IL was formed by a group of experienced raiders, guild leaders and officers that wanted to build a guild with the traditional values of what makes games fun. We believe that we can be one of the best guilds out there. We may never be top of the line or cutting edge but we will be a place you want to log into and hang out with friends.
We want to build this guild with good natured people. If we have a great group of people there will be a freindly/family atmosphere and everything else will take care of itself.
About 2 weeks before launch we will be voting on (as a guild) what type of server to play on. Once all the votes are in we will list the server names for the winning type and decide what server to play on.
From the beginning we will level and group with each other, exploring every aspect of AoC. For lower level people who come later, there is no need to worry, we will help people level as much as possible to catch people up.
The guild will be run by the Sacred Council. This will be comprised of an officer from every class and a couple of other positions. Every guild decision will be discussed and voted on by the council. When time of raiding comes about loot will be distributed fairly. If you are there and can use it you will have a chance to roll for it.
Once we have our guild city built we will focus heavily on both PVE and PVP aspects. We would like to get a guild city up and running and then shortly after that have a PVP keep up.
Guild - IL is in it for the common good, and not your personal bank account. A good player is one who joins the guild, says "hi", talks politely and acts mature and respectable. We're a social community, we want people to talk to, play with, and enjoy the game with. Not to say that we don't joke around, we are all adults we know where the line is. Casual - no attendance requirements (However, If you do not use your character or sign into the Guild Website at least 1 time every 30 days you risk being removed from the guild)
Guild requires the following:
-18+ years old (If you are under 18 you must be interview by the council)
-The ability to have fun with others
If these are the things you look for, then this is the right place for you.


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- physiocratic
- adjective see physiocrat
New Collegiate Dictionary. 2001.
New Collegiate Dictionary. 2001.
Physiocratic — Physiocrat Phys i*o*crat, n. [Gr. fy sis nature + ? to rule.] One of the followers of Quesnay of France, who, in the 18th century, founded a system of political economy based upon the supremacy of natural order. F. A. Walker. {Phys i*o*crat ic},… … The Collaborative International Dictionary of English
physiocratic — phys·i·o·crat·ic … English syllables
physiocratic — adjective see physiocrat … Useful english dictionary
PHYSIOCRATIC SCHOOL — a school of economists founded by Quesney, who regarded the cultivation of the land as the chief sources of natural well being, and argued for legislation in behalf of it … The Nuttall Encyclopaedia
physiocrat — physiocratic, adj. /fiz ee euh krat /, n. one of a school of political economists who followed Quesnay in holding that an inherent natural order properly governed society, regarding land as the basis of wealth and taxation, and advocating a… … Universalium
Physiocrats — • Describes the origins of Physiocratic theory Catholic Encyclopedia. Kevin Knight. 2006. Physiocrats Physiocrats † … Catholic encyclopedia
François Quesnay — Physiocrats François Quesnay, after a portrait by Jean Martial Frédou Born June 4, 1694(1694 06 04 … Wikipedia
Physiocrats — Physiocrats, Physiocratic Thought A school of social theory associated with the writings of the French political economist François Quesnay (1694 1774) and others. Physiocratic thought is mainly of interest to sociologists for its influence on… … Dictionary of sociology
Turgot, Anne-Robert-Jacques, baron de l'Aulne — born , May 10, 1727, Paris, France died March 18, 1781, Paris French administrator and economist. He entered the royal administrative branch of the magistracy in 1753, then became intendant (governor) of Limoges (1761–74), where he instituted… … Universalium
Europe, history of — Introduction history of European peoples and cultures from prehistoric times to the present. Europe is a more ambiguous term than most geographic expressions. Its etymology is doubtful, as is the physical extent of the area it designates.… … Universalium


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On Pearl Harbor Day, a Look at War Declarations
On the 70th anniversary of the Japanese attack on Pearl Harbor, it's useful to look back on the legal underpinnings of U.S. involvement in World War II. Back in the day, the U.S. used to "declare" war. More recently, Congress has "authorized" it. Sometime neither comes to pass.
In the absence of a declaration, the president is required to report to Congress withing 48 hours of introducing arm forces into hostilities and must pull them out within 60 days unless Congress permits otherwise, under the 1973 War Powers Resolution Act.
Presidents are required to file reports to Congress on military intervention and have done so 130 times since the law was passed, according to the Congressional Research Service. Of those, President Clinton submitted 60, President George W. Bush 39, and Obama five. . .


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Get an overview of this hotel3-star Birmingham hotel in Civil Rights Historic District, walk to Birmingham Museum of Art
Popular property highlights
A stay at Hampton Inn & Suites-Downtown-Tutwiler places you in the heart of Birmingham, walking distance from Birmingham Museum of Art and Don Kresge Memorial Museum.
This hotel is within close proximity of Birmingham Jefferson Convention Complex and Alabama Theater.
Make yourself at home in one of the 149 air-conditioned rooms featuring LCD televisions. Complimentary wireless Internet access keeps you connected, and cable programming is available for your entertainment. Bathrooms have showers and hair dryers. Conveniences include desks and complimentary weekday newspapers, and housekeeping is provided daily.
Enjoy the recreation opportunities such as a fitness center or make use of other amenities including complimentary wireless Internet access.
Grab a bite to eat at the hotel's restaurant, which features a bar, or stay in and take advantage of room service (during limited hours). Quench your thirst with your favorite drink at a bar/lounge.
Featured amenities include a 24-hour business center, express check-out, and dry cleaning/laundry services. Planning an event in Birmingham? This hotel features 3047 square feet (283 square meters) of event facilities. A roundtrip airport shuttle is complimentary (available 24 hours).Information missing or incorrect? Tell us!Opens in a new window
Hampton Inn & Suites-Downtown-Tutwiler
Hotel Amenities
Hotel Amenities
The hotel offers a restaurant. A bar/lounge is on site where guests can unwind with a drink. Guests can enjoy a complimentary breakfast. Public areas are equipped with complimentary wireless Internet access.
This 3-star property offers access to a 24-hour business center. Event facilities measure 3047 square feet (283 square meters). This business-friendly hotel also offers a fitness center, dry cleaning/laundry services, and room service (during limited hours). A roundtrip airport shuttle is complimentary to guests (available 24 hours). Onsite parking is available (surcharge).
Hampton Inn & Suites-Downtown-Tutwiler is a smoke-free property.
- Total number of rooms - 149
- Number of buildings/towers - 1
- Number of meeting rooms - 4
- 24-hour business center
- Number of floors - 8
- ATM/banking
- Free breakfast
- Bar/lounge
- Free airport transportation
- Elevator/lift
- Express check-out
- Fitness facilities
- 24-hour front desk
- Dry cleaning/laundry service
- Luggage storage
- Valet parking (surcharge)
- Safe-deposit box at front desk
- Conference space size (meters) - 283
- Conference space size (feet) - 3047
- Smoke-free property
- Free WiFi
- Restaurant
Family Friendly Amenities
- Family Hotel
- Free Wi-Fi
- Rollaway/extra beds available
- Cribs/infant beds (complimentary)
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Valet parking (surcharge)
Room Amenities
- Connecting/adjoining rooms available
- Free cribs/infant beds
- Room service (limited hours)
- Rollaway/extra beds available
- Shower only
- Iron/ironing board
- Air conditioning
- Free weekday newspaper
- Free local calls
- Coffee/tea maker
- Daily housekeeping
- Private bathroom
- Hair dryer
- Desk
- Cable TV service
- Free WiFi
Where to Eat
Guests are offered a complimentary hot/cold buffet breakfast.
Century Restaurant - This restaurant specializes in American cuisine and serves lunch and dinner. Guests can enjoy drinks at the bar.
Room service (during limited hours) is available.
Nearby Things to Do
Recreational amenities at the hotel include a fitness center.
If you have requests for specific accessibility needs, please note them at check-out when you book your room.
- Accessible bathroom
- In-room accessibility
- Roll-in shower
Hotel Policies
Minimum check-in age is 21
Check-in time ends at 4 AM
Check-in time starts at 4 PM
Special check-in instructions:
Check-out time is 11 AM
Payment types
Children and extra beds
- Children are welcome.
- Rollaway/extra beds are available.
- Free cribs (infant beds)!
- Pets not allowed (service animals welcome)
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Only service animals are allowed
No rollaway/extra beds available
Optional extras
- Valet parking fee: USD 24.00 per night
Hotel Name
- Hampton Inn Downtown-Tutwiler
- Hampton Inn Downtown-Tutwiler Birmingham
- Hampton Inn Downtown-Tutwiler Hotel
- Hampton Inn Downtown-Tutwiler Hotel Birmingham
We should mention
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.


text:
ED Magedson – Founder
Ripoff Report
Branch Banking and Trust Company200 WEST SECOND ST., Winston-Salem, NC 27102-1255 Luray, Virginia United States of America
Does your business have a bad reputation?
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Corporate Advocacy Program™
BB&T's Online Banking website is set up to intentionally trick and cheat its users. Once logged in and having selected your checking Account History, the page displays what should be accurate and complete information. It does not!
The 1st and most notable block in the upper left corner displays 2 items. Your "Available Balance" (bolded text) with the dollar amount and just below is the "Posted Balance" (un-bolded text), followed by that current dollar amount. The difference in the two values are presumably that the "Available Balance" includes pending transactions that will post by the end of that day and show up in the register below under the "Daily Posted Balance" column.
At the top of the register, are 3 tabs. The 1st one labeled Posted Transactions" is opened by default, and shows the list of all the normal recorded transactions down the page. [continued below]....
But here comes the deception. Any thinking person would now believe he has all the required knowledge of his checking account to know whether or not he has sufficient funds to make additional debits, either by making online payments or using his debit card at the store. However, this is where the fraud, treachery and the OVERFRAFT TRAP come into play. The false, misleading and incomplete information provided gives the user no reason, and just as importantly, no indication that he should look behind door #3, the 3rd tab labeled "Bill Payment Checks." If your doing online banking and not writing any checks, and you've already just been assured that you have viewed your current posted balance and all the pending transactions, there's no logical reason for you to click on the "Bill Payment Checks?"
As it turns out, these crafty b**tards apparently send out "hard" checks to some of your payees. And this tab is where those pending transactions are HID. Not only do they NOT get included in the "Pending Transactions" they typically languish under that tab, for many days longer than your other electronic payments. So now you continue your normal spending and depositing only to find out that those hidden transactions have now posted! So now you unknowingly have sent out maybe a dozen other payments, many of which have now overdrawn your account.
So now BB&T has tricked you by providing you with false account information that is intentionally incomplete, luring you into a false sense of security, for the sole purpose of slamming you with up to four, $35 overdraft fees per day. Those overdraft fees have now put your checking account even farther into the red, causing more overdrafts, more $35 fees etc., etc., and you are getting systematically ripped off while they greedily steal your money.
There must be tens if not hundreds of thousands of other BB&T Online bankers who have been and are continuing to be ripped off by BB&T. I would love to spearhead a class action law suite. Are there any lawyers reading this? Contact me please.
This report was posted on Ripoff Report on 10/04/2010 09:30 AM and is a permanent record located here: http://www.ripoffreport.com/reports/branch-banking-and-trust-company/luray-virginia-22835/branch-banking-and-trust-company-bbt-online-banking-website-designed-to-cheat-users-luray-647374. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
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text:
It was not mischief, was it, Mother, to try and capture trit?
trit and Trot were gone, and who could tell but the skirts and bonnets might not strangle them?
She now resolved to clutch at the first branch within reach, hoping in that way to scramble to safety with trit.
And then Anna had to tell again the story of her capture of trit.
Some time since I received from you one bottle Skookum chuck 3x trit.
He took with him two two-drachm phials of Blatta orientalis, one of IX and the other of 3x trit.
triticum, trit′i-kum, n. a genus of grasses including the varieties of wheat.
But trit also is doubtless originally the 'third string' rather than the 'third note.'
"We will put these on trit and Trot," she said, drawing out two white skirts from her collection of doll clothes.
I got a big phial full of water and put in it Blatta orientalis 1x trit.


text:
[Nature Republic] Crystal Eye Tint
Liquid type eyeshahow without powder for the better adhesion.
Multi creating shadow for tear drop liner, highlighter, eyeliner and shadow.
Natural color and pear with long-lasting effect.
#01 White Diamond, #02 Crystal Champagne, #03 Pink Pearls, #04 Rose Garnett, #05 Brown Topaz, #06 Midnight Sapphire
[How to Use]
Put a moderate amount to a tip and apply to the eyehole or the head of eyes.
Returns Policy
You may return most new, unopened items within 30 days of delivery for a full refund. We'll also pay the return shipping costs if the return is a result of our error (you received an incorrect or defective item, etc.).
You should expect to receive your refund within four weeks of giving your package to the return shipper, however, in many cases you will receive a refund more quickly. This time period includes the transit time for us to receive your return from the shipper (5 to 10 business days), the time it takes us to process your return once we receive it (3 to 5 business days), and the time it takes your bank to process our refund request (5 to 10 business days).
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text:
by Michele
We haven't been friends for more than 3 years or so, but during this time
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Data from: Replicate divergence between and within sounds in a marine fish: the copper rockfish (Sebastes caurinus)
Date Published: December 12, 2013
DOI: http://dx.doi.org/10.5061/dryad.s489b
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|Microsatellite DNA allele frequencies at 17 loci. Data are in base pairs and in CONVERT format
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text:
Sassy Glass Bead Necklace
Well the souk is full of jewelleries and still you are not satisfied. The pleasure of possession of a beautiful necklace is missing from long time. We can understand what your need is and therefore we are ready with eminent piece of collar.
Artisan Signature
- Artist Name: Suraj Mal


text: Accessory pouches are a stylish way to keep all your knick-knacks with you in one place when you're on the go. Featuring a double-sided print accessory pouch, you can choose between a standard bottom, or a wider "T" bottom one. Made from durable 100% polyester poplin fabric. Cold wash, no bleach. Dry low heat, Do not iron or dry clean.Start Selling Accessory Pouches


text:
I have one printer I cannot conenct to on a new Win7 workstation. I have the x64 driver installed on the network and when I connect to the printer (via login script) it asks to install the driver, gets to a point and then says it cannot connect, access denied. same thing when trying to manually install it.
Installing it locally and creating an IP port for the printer works.
Am I missing a rights setting somewhere? User is power user on machine. Even as a local admin though it still isnt working.
print server is 2003.
9 Replies
Feb 9, 2010 at 1:08 UTC
Check a network share permissions where drivers reside (add everyone and authenticated user) just in case, and give them <change/modify rights>. Go on print-server to properties of the printer itslf, and add everyone and authenticated user as well (give them full rights for now/to test). Vista Business OS had similar issues in 2008 and triggered a "spool services error", but that we had to tweak it work around for a while when we install a local printer/drivers and added IP port on Vista client.
Try above, that should work.
Feb 9, 2010 at 2:06 UTC
Thats what I would have said.
Feb 9, 2010 at 2:21 UTC
What do you mean by "check a network share permissions"?
Giving full rights on printer not working.
Feb 9, 2010 at 2:39 UTC
Try to run this command on win-7 machine.
bcdedit /set loadoptions DDISABLE_INTEGRITY_CHECKS
Than try adding printer driver again
Feb 9, 2010 at 2:51 UTC
The driver shows installed on the comptuer (b4 running the command). Acutally installing the printer is what's not working.
after running the command i got a 0x00000007e error.
Feb 9, 2010 at 4:05 UTC
So here's a curiosity, I manually added the printer. In the wizard, I found the printer via IP address. I then didn't let the system choose the driver, manually picked the matching PCL and it installed fine. BUT, my win7 machine saw it as some 2025 (?) color laser; the printer in questino is a 5000. A test page did print fine.
If I locate the printer in the directory or select it by name it errors.
Feb 10, 2010 at 8:18 UTC
i it's an HP laser manually add it and use the HP LaserJet 1100 print drivers they will work for any B&W Hp Laser printer.
Feb 10, 2010 at 8:49 UTC
I don't think it's necessarily a driver issue since I can add it manually using the recommended driver (which is the universal PCL).
Feb 12, 2010 at 8:19 UTC
I tried everything with no success so I just manually added the printer to the computer and took it out of the login script. It's only for 4 machines so not a huge deal.
Thanks for the suggestions!
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
Welcome to Schallenberger Elementary
Please call 408-287-6257 to report your child's absence.
Please do not call the office.
Parent Welcome Letter
Welcome to the 2017-18 school year! The entire Schallenberger staff extends a warm welcome to our new and returning families. Teachers have been busy preparing their classrooms and curriculum for new learning experiences for your children. The first day of school is WEDNESDAY, AUGUST 16. In the meantime, here are a few notes about the opening of the 2017-18 school year.
Parent Welcome Letter
¡Bienvenidos al ciclo escolar 2017-2018! Todo el personal de Schallenberger extiende una cálida bienvenida a las familias nuevas y a las que regresan. Los maestros han estado ocupados preparando sus salones de clases y el plan de estudios para las nuevas experiencias de aprendizaje de sus hijos. El primer día de clases será el MIÉRCOLES, 16 DE AGOSTO. Mientras tanto, estas son algunas notas sobre la apertura del ciclo escolar 2017-2018.
Carta de Bienvenido Para Los Padres
Our School Vision
Vision Statement
All San Jose Unified School District students are inspired and prepared to succeed in a global society.
Mission Statement
San Jose Unified School District's mission is to eliminate the opportunity gap and provide every student with the finest 21st century education.
Our Goals
•A safe, nurturing environment is the foundation for the Schallenberger learning community.
•Students develop into confident, productive, responsible, lifelong learners with the skills to succeed in an ever changing world.
•Teachers and staff use effective, research-based instructional strategies to engage every student in academic and social success.
•Students learn the skills necessary to communicate effectively, work collaboratively, problem solve, and develop their creativity
Quick Links
School Accountability
Report Card (SARC)
Informes Escolares
de Rendición de Cuentas (SARC)
Contact Principal
Ryan Chamberlin, Principal
Attendance Hotline
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Three-Minute Video Explaining
the Common Core State Standards
Common Core and Growth Mindset for parents, teachers, and students
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Student Handbook
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Enrollment Information
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Anti-Bullying Policy and Plan Please visit the updated School Climate and Safety page.
Recommended Classroom Supplies/Materiales escolares recomendados
On Campus before and after School Care provided by the YMCA. Please call 408-445-8556 for more information.
After School Care provided by ROCK (run by the City of SJ). Please call 408-448-6400 for more information.


text:
Fiddlesticks Build Guide by tidid
Not Updated For Current Season
This guide has not yet been updated for the current season. Please keep this in mind while reading. You can see the most recently updated guides on the browse guides page.
Fiddlesticks Build
Purchase Order
Ability Sequence
Not Updated For Current Season
The masteries shown here are not yet updated for the current season, the guide author needs to set up the new masteries. As such, they will be different than the masteries you see in-game.
Chapter 1
First buy doran's rings. After rabadon's sell one doran ring and buy rylai. Next sell second doran's and buy morello. Same with third doran's. It's THE BEST build cause u sucks so much hp on low lvl. I fought with many fiddlesticks who starts with rylai or rod of ages. They had much hp but i suck more hp than they. So they die faster.


text: If members can see other members browsing gallery & entries, then this gives a sense of activity which motivates people to participate. Especially if the function refreshes inline through jquery events, because then it gives a sense of live participation which is generally a better experience than static participation. @0ptima


text:
Saint-Denis, France: Sidney Govou scored two goals and Thierry Henry added another on Wednesday to give France a 3-1 win over Italy in a European Championship qualifier. Govou scored in the second minute, and Henry added another in the 17th for his 37th international goal - moving him within four goals of Michel Platini's French record. Govou scored again in the 56th when he headed home Willy Sagnol's cross. Alberto Gilardino scored for Italy in the 20th. Just two months after the teams met in the World Cup final, France had several chances to extend its lead before the interval. "The perfect match is where you score on every attack and where you are never in danger," France coach Raymond Domenech said. "I have to admit we weren't far from the perfect match." France has six points from two matches in Group B, behind Scotland on goal difference. Italy has only one point from its two matches. "We have beaten two World Cup winners in the last two months," said Domenech, referring to the World Cup quarterfinal win over Brazil. "But what's more important is that we have distanced Italy by five points." Domenech told his players not to get carried away by the win. "Let's not think we have already qualified," Domenech said. "We need to stay calm. But if we win in Scotland, then we will be in a strong position in the group." Despite pleas from Domenech, fans began booing when the Italian national anthem started. With the win secured, fans at Stade de France broke out into chants of "Zizou, Zizou" in homage to Zinedine Zidane, who was red-carded in the World Cup final for head-butting Marco Materazzi and retired after the match. With two minutes gone, Henry sent the ball left to William Gallas and his dipping cross was volleyed back the same way and into the corner of the net by Govou - who only started because Louis Saha was not fully fit. Gianluca Zambrotta shot over the bar in the fifth minute as Italy sought a quick reply, but it was a rare attack. Franck Ribery's 12th-minute cross, again from the left, eluded Italy's defenCe, but also his own teammates. Three minutes later, Italy survived again when Henry got into the penalty area and Zambrotta had to scramble his cross away before Florent Malouda could tap it in. Henry ended a seven-game scoreless streak when he curled the ball under Gianluigi Buffon from 12 meters after the goalkeeper had blocked Malouda's powerful shot. With French fans singing, Italy quickly pulled a goal back when Gilardino was left unmarked to head in a free kick from Andrea Pirlo with goalkeeper Gregory Coupet stranded. Gennaro Gattuso avoided a caution when he clattered into the back of France captain Patrick Vieira, and Sagnol then forced Buffon into a save at his left-hand post in the 26th. With the action almost incessant, Coupet clawed the ball off the line, and Italy captain Fabio Cannavaro was cautioned for flattening Ribery as the winger sped past. Italy coach Roberto Donadoni handed a second appearance to Chievo midfielder Franco Semioli, and Andrea Barzagli stepped in for the suspended Materazzi, with Alessandro Nesta still out injured. Stronger in the tackle, France controlled midfield with Claude Makelele in impressive form alongside Vieira. A fine move saw Makelele sweep the ball to Sagnol, and the Bayern Munich defender's curling cross was powerfully headed in by Govou for his fifth goal in 24 games. He almost added a third in the 65th after collecting Henry's pass, but shot wide. Coupet then made a brilliant reaction save to keep out Cannavaro's header, and Gilardino was booked by referee Herbert Fandel for diving. (AP)
Topics : Football France Romania Greece Lyon Costa Rica
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text: To get from Ascoli Piceno a Nicastro by train you can catch one of the 2 trains that everyday travel between S. Filippo and Lamezia Terme-Nicastro, the closest station to the city. The first train to Lamezia Terme-Nicastro leaves from S. Filippo at 16:06 and the last train departs at 20:53. If you choose a local train the trip lasts on average 14:59 hours. On Wanderio you can compare all schedules, fares and railway companies to Lamezia Terme-Nicastro and book the best itinerary starting from S. Filippo.


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|Phenidate drug formulations having diminished abuse potential
|Phenidate drug formulations are provided having reduced potential for drug abuse. Dosage forms for treating Attention Deficit Disorder, Attention Deficit Hyperactivity Disorder, AIDS Dementia Complex and cognitive decline in HIV-AIDS are provided which minimize drug hypersensitivity, toxicity, side effects, euphoric effect, and drug abuse potential. Such dosage forms comprise D-threo stereoisomer of a phenidate in the substantial absence of all other stereoisomers.
|Zeitlin; Andrew L. (Millington, NJ), Dariani; Maghsoud M. (Fanwood, NJ)
|Celgene Corporation (Warren, NJ)
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text:
Athens County, Ohio rural scene with an old barn next to a road.
Athens CountyOhio
The Ohio University gate to the campus in Athens, Ohio
A jet Ski speeds up the Ohio River next to the Athens County shoreline.
The Nelsonville Train station is a tourist attraction where tourists can take a scenic train ride through hilly and rural Athens County, Ohio countryside.
Athens CountyOhiotrains
The Nelsonville, Ohio town square.
The Ohio University main campus building in Athens, Ohio.
The home of Rocky Boots in Nelsonville, Ohio.
The Sunset Motel in Athens, Ohio.
Athens CountyOhioold motel
A railroad snow plow is kept at the Nelsonville, Ohio train station.
The front of a giant snow plow train car at the Nelsonville, Ohio Train Depot.
A college apartment roof built for sunning and partying in Athens, Ohio.
Athens CountyOhiodorms
Restored caboose at the Nelsonville, Ohio Train Depot
Athens CountyOhiotrain
A massive Marlin bird house in rural Athens, Ohio.
Athens CountyOhiobirdhouse
The Blackwood Covered Bridge in Athens County, Ohio
Athens CountyOhiocovered bridge
Side view of the Blackwood Covered Bridge in Athens County, Ohio
Downtown Athens, Ohio graffiti.
Athens CountyOhiograffiti
Old style diner in downtown Athens, Ohio near the Ohio University campus.
Athens CountyOhiodiner
Repair work being done on the Athens County Courthouse in downtown Athens, Ohio
Athens CountyOhiocourthouse
Rural Athens County, Ohio scene with hay bales and the Blackwood Covered Bridge.
Athens CountyOhiohaycovered bridge
Covered bridge in northern Athens County, Ohio
Glouster, Ohio mural dedicated to the towns history of railroads and mining.
Athens CountyOhioGloustermural
Remnants of the Glouster, Ohio Hotel.
Athens CountyOhiohotel
Downtown Glouster, Ohio.
Athens CountyOhioglouster
Northern Athens County, Ohio covered bridge.
The Athena Theater in Athens, Ohio
Athens CountyOhiotheater
Decaying Building in Trimble, Ohio
Decaying old building in Trimble, Ohio.
rural decayAthens CountyOhio


text:
All Android applications categories
This app gives information on Tips for gardening.Save all flats and flower pots that come with your plants.
this app contains
Gardening Catalog
Gardening Tips
Garden Weddings
Landscaping Design
and more..................
Q: Why does it need INTERNET permission?
A: This is a free app, we need ads to support our work.
** This is an Information app.
** Giving low rating won't improve the app. Low rating or vague comments won't help us know what you find missing in the app. If you think the app has potential, start a dialog! Email us and help us take the app to the next level. Your email will be promptly responded to and acted on!
We work hard to improve the app, and we need your help.
gardening hints and tips,
vegetable gardening tips,
beginners gardening tips,
organic gardening tips,
Gardening Catalog,
Gardening guide,
Organic Gardening.
Download Tips for gardening
Free - V1.0 - 8M
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text:
It only takes a minute to get started. Fill out our simple sign up form and login to the website.
This step is up to you but will drastically increase your chances of your profile being viewed by others. Introduce yourself, your interests and upload some good photographs of yourself.
Use our website's advanced search to pinpoint members who meet your requirements. Or just look through listings and pinpoint singles that you like.
Located someone who tickles your fancy? Send a message to them or just a wink and see if they're interested. With a little luck you'll be on your way to locating your proper match.
You won't regret it


text:
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text:
The Facioscapulohumeral muscular dystrophy region on 4qter and the homologous locus on 10qter evolved independently under different evolutionary pressure
The homologous 4q and 10q subtelomeric regions include two distinctive polymorphic arrays of 3.3 kb repeats, named D4Z4. An additional BlnI restriction site on the 10q-type sequence allows to distinguish the chromosomal origin of the repeats. Reduction in the number of D4Z4 repeats below a threshold of 10 at the 4q locus is tightly linked to Facioscapulohumeral Muscular Dystrophy (FSHD), while similar contractions at 10q locus, are not pathogenic. Sequence variations due to the presence of BlnI-sensitive repeats (10q-type) on chromosome 4 or viceversa of BlnI-resistant repeats (4q-type) on chromosome 10 are observed in both alleles.
We analysed DNA samples from 116 healthy subiects and 114 FSHD patients and determined the size distributions of polymorphic 4q and 10q alleles, the frequency and the D4Z4 repeat assortment of variant alleles, and finally the telomeric sequences both in standard and variant alleles.
We observed the same frequency and types of variant alleles in FSHD patients and controls, but we found marked differences between the repeat arrays of the 4q and 10q chromosomes. In particular we detected 10q alleles completely replaced by the 4q subtelomeric region, consisting in the whole set of 4q-type repeats and the distal telomeric markers. However the reciprocal event, 10q-type subtelomeric region on chromosome 4, was never observed. At 4q locus we always identified hybrid alleles containing a mixture of 4q and 10q-type repeats.
The different size distribution and different structure of 10q variant alleles as compared with 4q suggests that these loci evolved in a different manner, since the 4q locus is linked to FSHD, while no inheritable disease is associated with mutations in 10qter genomic region. Hybrid alleles on chromosome 4 always retain a minimum number of 4q type repeats, as they are probably essential for maintaining the structural and functional properties of this subtelomeric region.
In addition we found: i) several instances of variant alleles that could be misinterpreted and interfere with a correct diagnosis of FSHD; ii) the presence of borderline alleles in the range of 30–40 kb that carried a qA type telomere and were not associated with the disease.
DNA repeats can be the source for an extraordinary amount of information about biological processes. As passive markers they provide clues for evolutionary events and processes of mutation and selection, as active agents repeats have remodeled the genome by causing ectopic rearrangements, by creating entirely new genes and by modifying and reshuffling existing genes. Specifically, segmental duplications involve the transfer of 1–200 kb blocks of genomic sequences to one or more locations in the genome and may contain various types of repeats
In this paper we performed a detailed analysis of 4q and 10q subtelomeric regions by characterizing the D4Z4 repeat assortment and the distal telomeric markers in Italian healthy subjects and FSHD patients, thus extending previous studies in other populations. We determined: i) the size distribution of polymorphic 4q alleles as compared with 10q alleles; ii) the frequency of variant 4q and 10q alleles; iii) the D4Z4 repeat assortment of 4q and 10q variant alleles; and finally; iv) the qA and qB telomeric sequences both in standard and variant alleles.
Patients and controls Diagnostic criteria for FSHD followed the guidelines proposed by the European Expert Group on FMD
Lymphocytes isolation from whole blood
Lymphocytes were isolated from peripheral blood sample, embedded in 0.5% Low Melt Agarose (Bio-Rad, Hercules, CA, USA) plugs and incubated with 0.5M EDTA, 1% Sarcosyl (Sigma, St Louis, MO, USA) and 1 mg/ml Proteinase K for 24–48 hrs at 50°C (Roche, Mannheim, Germany), according to the procedure described by Pharmacia.
Restriction endonuclease digestion of DNA in agarose blocks and PFGE
1/4 of plug, corresponding to 0.5 × 106 cells, was digested with the following restriction enzymes: EcoRI (Roche, Mannheim, Germany), BlnI (Amersham Pharmacia Biotech, Buckingamshire, U.K.) and XapI (Fermentas). Single (EcoRI, XapI) and double digestions (EcoRI/BlnI) were performed with 60/150 U of enzyme added in three equal portions, at 1h intervals and incubated overnight.
PFGE was performed at 10°C in a Pulsaphor Electrophoresis unit with HEX electrode (Pharmacia LKB). High Strength Analytical Grade Agarose (Bio-Rad, Hercules, CA, USA) was used to prepare 1.2% gel in 0.5 tris-borate-EDTA. The running procedure was performed at 300 V in 4 cycles: the first cycle was 1 h 30' with pulse of 0.3 s; the second was 3 h 30' with pulse of 0.5 s, the third was 4 h with pulse of 1 s and the fourth was 2 h 30' with pulse of 5 s
Southern Blot analysis
After electrophoresis the DNA was transferred to Nylon membrane Hybond N+ (Amersham Pharmacia Biotech, Buckingamshire, U.K.) for hybridization. The probes were labelled by random priming with [α-32P]ATP using the Multiprime DNA labeling system (Amersham Pharmacia Biotech, Little Chalfont, UK). Both the single copy p13E-11 probe and the KpnI repeat sequence were obtained from 4.9 kb KpnI fragment subcloned in pUC21
qA and qB telomeric markers
To perform the allele-typing for qA and qB, the DNA of healthy and affected subjects was digested with HindIII restriction enzyme (Roche Mannheim, Germany) (Fig.
Figure 1
4q and 10q subtelomeric region
4q and 10q subtelomeric region. Positions of DNA probes and restriction enzymes utilized in the study are indicated.
Statistical analysis
The mean values of 4q and 10q allele sizes derived from healthy and FSHD subjects were compared using Mann-Whitney U-test and T-student test. The size distribution of 4q and 10q polymorphic fragments showed a bimodal distributions when divided into 20 kb classes. Upon visual inspection of the distribution we decided to partition each of them in 2 clusters. Cluster centers and number of chromosomes falling in each cluster were obtained with the program Quickcluster as implemented in SPSS v. 6.0.
Canonical and non canonical pattern of 4q and 10q alleles in human subjects
We defined an individual as carrying a canonical pattern of alleles when, after PFGE analysis and hybridization with p13E-11 probe, we observed four restriction fragments with EcoRI and only two restriction fragments after double digestion EcoRI/BlnI: the alleles of 10q origin containing BlnI sensitive repeats were converted into 2.8 kb fragments and run out of the gel, while the alleles of 4q origin containing BlnI-resistant repeats were preserved and reduced by 3 kb due to a single BlnI restriction site 3 kb distal to the proximal EcoRI restriction site (Fig.
Size distribution of 4q and 10q alleles
Healthy individuals (Fig.
Figure 2
Size distribution of 4q and 10q alleles in healthy and FSHD affected subjects
Size distribution of 4q and 10q alleles in healthy and FSHD affected subjects. The size distributions of 4q and 10q alleles detected in healthy subjects (panel a) and FSHD patients (panel b) are represented. The size of EcoRI fragments is expressed in kb, with 10 kb intervals. Black bars: 4q wild-type alleles; grey bars: disease-associated 4q alleles; white bars: 10q alleles.
Only 67% of 106 healthy subjects showed, after p13E-11 hybridization, a canonical pattern of alleles (Tab.
Table 1
Canonical and non canonical patterns in healthy subjects and FSHD patients
- Canonical pattern
71 (67%)
51 (73%)
- Non canonical pattern
35 (33%)
19 (27%)
Total number
FSHD familial cases (Fig.
Among 70 FSHD probands, 73% showed a canonical patterns. The mean value of the sizes of the 4q alleles not implicated in the disease was 76.73 ± 33.90 kb, significantly lower than that found in the controls carrying a pair of wild-type 4q alleles (p = 0.037 by T Student test and p = 0.042 by Mann-Whitney test). This result was in contrast with the even distribution of the size of 10q alleles in both healthy and affected subjects. The mean value of the size of the disease- related allele was 25.12 ± 5.37 kb.
qA and qB Telomeric markers
A biallelic variation, named qA and qB type, was identified at 4q telomeric region distal to D4Z4
Table 2
Typing of qA and qB telomeres of 4q alleles from healthy subjects and FSHD patients
Healthy subjects
FSHD patients (normal allele)
FSHD patients (short allele)
45 (68%)
21 (32%)
23 (57%)
17 (43%)
40 (100%)
0 (0%)
TOT: 66 alleles
TOT: 40 alleles
TOT: 40 alleles
Table 3
Number and type of variant alleles in healthy subjects and FSHD patients
Chr. 4 alleles
Chr. 10 alleles
Total number of alleles
Variant alleles (total)
homogeneous KpnI array
heterogeneous KpnI array
p13E-11 deletion
*only one variant 4q allele is disease-related
Among healthy subjects we observed two 4q alleles of 33 and 38 kb (Fig.
Figure 3
4q and 10q allelic pattern of a FSHD family after hybridization with p13E-11 and qA-qB telomeric markers
4q and 10q allelic pattern of a FSHD family after hybridization with p13E-11 and qA-qB telomeric markers. a) DNA samples were digested with EcoRI (E) and EcoRI/BlnI (E/B) enzymes, separated with PFGE and hybridized with p13E-11 probe. The proband (I:1) shows a trisomic pattern with two 4q alleles of 18 and 30 kb and two 10q alleles of 41 (BlnI-resistant) (*) and 48 kb (BlnI-sensitive). The affected son (II:1) is also trisomic and inherited from the father the 4q FSHD allele of 18 kb and the10q of 41 kb (*) and from the mother the 4q of 53 kb and a similar size10q allele. The unaffected daughter inherited from the father the 4q allele of 30 kb and the 10q BlnI-resistant allele of 41 kb (*) and from the mother the 4q and 10q fragments of 53 kb. The segregation data for the probe D4S139 showed that II:1 and II:2 inherited the same 4q allele from the mother (I:2), but different 4q alleles from the father (I:1). This suggest that the BlnI-resistant allele of 41 kb is a 10q variant; b) DNA samples were digested with HindIII (H), separated with PFGE and subsequently hybridized with qA and qB probes: the short 4q allele (18 kb) is qA type, while the 4q allele of 30 kb is qB type. All the standard 10q alleles of 48, 53 and 20 kb have qA type telomeres, while the variant 10q allele of 41 kb is qB type. This variant 10q allele carries the Bln1-resistant repeat array and the distal telomeric sequence of 4q-type. The 10q origin of this allele is confirmed by absence of segregation with D4S139 probe. The DNA sample of the subject II:2 was not sufficient for qA/qB Southern Blot analysis and it was not included in figure 3. In the diagram below the alleles observed after EcoRI and EcoRI/BlnI digestion are shown: the 4q alleles are in bold and the telomeres (A or B) are in brackets.
4q and 10q allelic patterns in healthy subjects and FSHD patients
In healthy individuals we found a change in the disomic pattern of alleles in 35 cases (33%): 10 (9%) were trisomic, 16 (15%) monosomic and 7 (7%) displayed more complex rearrangements (Tab.
Among the 70 FSHD probands we found that 73% carried a canonical pattern of alleles (Tab.
Figure 4
Nullisomic pattern
Nullisomic pattern. The patient shows four EcoRI bands after p13E-11 hybridization and none after double digestion with EcoRI/BlnI (nulisomic pattern). After hybridization with KpnI probe two BlnI resistant band are visible (45 and 20 kb): we can not guess the chromosomal origin of these alleles. To resolve the structure of the alleles we digested the sample with XapI enzyme which cuts the 4q-type repeats only (see Methods). After hybridization with p13E-11 we observed 4 XapI-resistant bands (38, 32, 13 and 8 kb): the 38 and 32 kb bands correspond to the EcoRI fragments of 42 and 36 kb fragments (XapI fragments are 4 kb smaller than EcoRI fragments). On the contrary the 13 and 8 kb fragments are stretches of the hybrid 4q alleles: the 62 kb EcoRI allele is composed by a BlnI-sensitive fragment (XapI-resistant) of 13 kb and a BlnI-resistant fragment of 45 kb (13+45 = 58 kb). The 32 kb EcoRI allele is composed by a BlnI-sensitive fragment (XapI-resistant) of 8 kb and a BlnI-resistant fragment of 20 kb (8+20 = 28 kb). The 4q variant alleles are represented in figure 5 (n.15 and 16). The FSHD 4q allele of 32 kb segregated also in other affected members of the family and its origin was confirmed by D4S139 probe (data not shown). * aspecific bands.
Analysis of structural organization of variant 4q and 10q alleles in healthy and affected subjects
After hybridization with p13E-11, the filters were stripped and re-hybridized with the multicopy KpnI probe (see Methods). If the 4q and 10q alleles were homogeneous in D4Z4 repeat constitution, KpnI hybridization confirmed the canonical pattern observed with p13E-11. On the contrary, if the 4q and 10q alleles had a non homogeneous repeat array, additional bands were observed after hybridization with KpnI (Fig.
In more complex cases and where we could not study the allelic segregation
In conclusion we named as "standards" those allele in which both the 4q and 10q alleles carried a homogeneous, uninterrupted array of BlnI-resistant (4q-type) or BlnI-sensitive (10q-type) repeats, respectively (Fig.
In healthy subjects we analysed a total number of 212 alleles of either 4q or 10q types and identified 19 4q variant alleles (9%) and 13 10q variant alleles (6%) (Tab.
A similar frequency was observed in the affected individuals: among the 140 alleles analysed, 8 variant 4q (6%), and 13 variant 10q (9%) alleles were encountered (Tab.
In figure
Figure 5
Analysis of structural organization of variant 4q and 10q alleles in healthy subjects and FSHD patients
Analysis of structural organization of variant 4q and 10q alleles in healthy subjects and FSHD patients. White bars: BlnI-sensitive repeats; Black bars: BlnI-resistant repeats. Variant 4q alleles: N° 1–13 refer to healthy subjects (H), N° 14–20 to FSHD patients. Variant 10q alleles: N° 1–4 refer to healthy subjects (H), N° 5–12 to FSHD patients.
4q variant alleles (10q repeats on chromosome 4q)
We observed BlnI-sensitive repeats upstream the BlnI-resistant units on 4q chromosome in 26 out of 27 variant alleles (Fig.
Among FSHD patients we found one case in which the disease allele was hybrid and carried BlnI-sensitive repeats mimicking a 10q allele (allele n.16 Fig.
The size of the variant 4q alleles ranged between 40 and 200 kb (corresponding to 10 and 60 repeat units) and there was no preferential involvement of a specific class of alleles. In 14 cases a small number of 10q-type repeats were present on 4q alleles (from 1 to 3 repeats). In 4 alleles (10-11-12-17) we found evidence of multiple clusters of BlnI-sensitive repeats interspersed within BlnI-resistant units.
10q variant alleles (4q repeats on chromosome 10q)
In 25 out of 26 cases (96%) BlnI-resistant D4Z4 repeats were detected on the 10q chromosome at the proximal level, close to the p13E-11 site (Fig.
Telomeric markers
We were interested to extend the study of qA and qB telomeric sequences to 4q and 10q variant alleles. We analysed twelve 4q variant alleles: nine of them showed an A type telomere, three a B type. Among the twelve 10q variant alleles studied carrying a homogeneous set of BlnI-resistant repeats, eight were of A type and four of B type (Fig.
Figure 6
10q alleles with qB telomere
10q alleles with qB telomere. Allelic pattern of individuals carrying 10q alleles with a qB type telomere. a) The segregation study with the D4S139 probe demonstrates that the two sisters inherited the same 4q alleles of 150 and 60 kb. I:1 shows a trisomic pattern with a BlnI-resistant 10q of 32 kb (*) and a standard 10q of 45 kb. The analysis of the telomeric sequences reveals that the variant 10q allele of 32 kb has a qB telomere. The two 4q alleles and the standard 10q allele of 45 kb have qA telomeres. b) The patient is trisomic and carries a FSHD allele of 33 kb and a standard 10 q allele of 70 kb with a qA telomere. We can not distinguish the 10q variant allele from the 4q standard alleles. Since both the 85 kb and 55 kb show a qB telomere, we deduce that the variant 10q allele carries a qB telomere. c) The 4q short allele of 20 kb segregates with FSHD in the family. The affected daughter (II:1) is trisomic and inherited from the mother (I:1) both the FSHD-linked 4q of 20 kb and the BlnI-resistant 10q allele of 49 kb (*) and from the father a standard 10q allele of 70 kb and a 4q standard allele of 100 kb. The analysis of the telomeric sequences shows that the variant 10q allele of 49 kb has a qB telomere, while the 10q standard allele of 70 kb and both 4q alleles of 100 and 70 kb have a qA telomere. The 10q variant allele of 49 kb is represented in figure 5 (n.11).
The high level of sequence homology between the 4q and 10q loci would imply for both polymorphic regions a similar size distribution and recombination products. This is not the case: 10q alleles displayed a different size distribution as compared with 4q alleles with a high percentage of alleles shorter than 40 kb (18%). The differences are more evident when we analysed the structure of variant alleles. 4q variant alleles were heterogeneous and always contained a mixture of BlnI-sensitive (10q-type) and BlnI-resistant (4q-type) repeats, on the contrary 10q variant alleles usually displayed a homogeneous set of BlnI-resistant (4q-type) repeats. The variant alleles revealed that such interactions probably occurred in the past, but the different structures of 4q and 10q variant alleles support the idea that the exchanges between the two loci are rare and these subtelomeric regions appear to evolve independently under different evolutionary pressure. Since reduction in the number of D4Z4 on 4q locus results in FSHD, it is not surprising that 4q chromosomes have incurred a strong selection pressure during human evolution. Gabellini et al. reported that a repressive protein complex binds a specific sequence within each D4Z4 repeat unit on chromosome 4. The reduction in the number of repeats in FSHD patients abolishes this binding and results in the loosening of the transcriptional repression at 4q region and in the overexpression of upstream genes (FRG2, FRG1, ANT1)
Moreover we described qB telomeres on 10q variant alleles carrying homogeneous 4q-type repeat arrays. This suggests that qB sequences are not exclusively associated with 4q chromosomes, but they are always linked to BlnI-resistant repeats.
It was proposed that an ancestral duplication event generated 4q and 4p telomeric regions, then 4q sequences diverged and gave origin to a 4qA type telomere that was duplicated onto chromosome 10. A more recent duplication of telomeric sequences from 4p onto 4q gave origin to 4qB-type telomere
We reported a detailed molecular analysis of the 10q and 4q subtelomeric regions in FSHD patients and controls with the aim of characterizing the D4Z4 repeat array assortment and the distal telomeric region (qA and qB) of 4q and 10q alleles. We obtained new interesting findings about the structure of these subtelomeric regions that should be taken into account for a correct molecular diagnosis and genetic counseling of FSHD. In some cases the chromosome origin of the p13E-11 fragments could be misinterpreted: i) we found several 10q variant alleles with a BlnI-resistant array and a size smaller than 40 kb (Fig.
Furthermore we studied in a different population the 4qter polymorphism distal to D4Z4 described in a previous Dutch study
Facioscapulohumeral Muscular Dystrophy: FSHD
Competing interests
The author(s) declare that they have no competing interests.
Authors' contributions
MR performed laboratory work, results collection and wrote the paper. ER, GG and LF designed and coordinate the study. PAT recruited subjects and participated in clinical diagnosis, LC performed laboratory work and results collection. RF recruited subjects. LF supervised the study and wrote the paper.
All authors read and approved the final manuscript.
We are most grateful to Prof. Andrea Novelletto for the statistical analysis, Patrizia Venditti, Mario Pescatori and Silvere M. van der Maarel for helpful discussions. This work has been supported by Telethon Italy grant No. 1296, by UILDM Rome Section, Italy and partially by Italian MIUR and Italian Ministry of Health.
Masquerading repeats: paralogous pitfalls of the human genomes
An anthropoid specific segmental duplication on human chromosome 1q22
Large multi-chromosomal duplications encompass many members of the olfactory receptor gene family in the human genome
Interchromosomal duplications of the adrenoleuko-dystrophy locus: a phenomenon of pericentromeric plasticity
Human subtelomeres are hot spots of interchromosomal recombination and segmental duplication
A Cascade of Complex Subtelomeric Duplications during the Evolution of the Hominoid and Old World Monkey Genomes
Genomic analysis of human chromosome 10q and 4q telomeres suggests a common origin
Physical mapping evidence for a duplicated region on chromosome 10qter showing high homology with the FSHD locus on chromosome 4qter
Regional mapping of facioscapulohumeral muscular dystrophy gene on 4q35: combined analysis of an international consortium
Chromosome 4q DNA rearrangements associated with facioscapulohumeral muscular dystrophy
Definitive molecular diagnosis of facioscapulohumeral dystrophy
Progress in the molecular diagnosis of facioscapulohumeral muscular dystrophy (FSHD) and correlation between the number of KpnI repeats at the 4q35 locus and clinical phenotype
Direct detection of 4q35 rearrangements implicated in facioscapulohumeral muscular dystrophy (FSHD)
Sequence homology between 4qter and 10qter loci facilitates the instability of subtelomeric KpnI repeats units implicated in facioscapulohumeral muscular dystrophy
Interchromosomal repeat array interactions between chromosomes 4 and 10: a model for subtelomeric plasticity
Inter- and intrachromosomal sub-telomeric rearrangements on 4q35: implications for facioscapulohumeral muscular dystrophy (FSHD) aetiology and diagnosis
Facioscapulohumeral muscular dystrophy is uniquely associated with one variants of 4q subtelomere
Contraction of D4Z4 on 4qB subtelomere do not cause Facioscapulohumeral muscular dystrophy
Diagnostic criteria for facioscapulohumeral muscular dystrophy
Pulse field gel electrophoresis for the detection of facioscapulohumeral muscular dystrophy gene rearrangements
Chromosome 4q35 haplothypes and DNA rearrangements segregating in affected subjects of 19 Italian families with Facioscapulohumeral muscular dystrophy (FSHD)
Pulsed-field gel electrophoresis of the D4F104S1 locus reveals the size and the parental origin of the facioscapulohumeral muscular dystrophy (FSHD)-associated deletions
Mechanism and timing of mitotic rearrangements in the subtelomeric D4Z4 repeat involved in facioscapulohumeral muscular dystrophy
Possible phenotypic dosage effect in patients compound heterozygous for FSHD-sized 4q35 alleles
Monosomy of distal 4q does not cause facioscapulohumeral muscular dystrophy
DNA rearrangements in Japanese facioscapulohumeral muscular dystrophy patients: clinical correlations
Complete allele information in the diagnosis of facioscapulohumeral muscular dystrophy by triple DNA analysis
Inappropriate gene activation in FSHD: a repressor complex binds a chromosomal repeat deleted in dystrophic muscle
Facioscapulohumeral muscular dystrophy in mice overexpressing FRG1
Pre-publication history
The pre-publication history for this paper can be accessed here:


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SDS GS LPG Rail Tank Car 2 Pk
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SDS GS LPG Rail Tank Car 2 Pk
NSWR GS LPG Rail Tank Car
10 of these wagons were built by Commonwealth Engineering at Granville, under licence to the Union Tank Car Co., between 1964 to 1967 and were numbered GS400 to GS409. They were privately owned by the Gas Supply Co. Ltd of Melbourne and painted in their retail brand name of Speed-E-Gas. As Boral had acquired the Gas Supply Co., in 1964 a small Boral logo appeared on each end of the tank.
These were the first privately-owned wagons to be approved for operation on broad and standard gauge tracks through the bogie exchange plant at Dynon. In 1974 three wagons were sent to Victoria and re-branded Heatane Gas later in 1978 a fourth wagon was re-branded Aftrans to carry Anhydrous Ammonia.
A comprehensive article by Neil Cram appeared in the Australian Model Railway Magazine in October 1989 detailing the colourful history of these wagons.
See the table below for wagon transfer dates and renumbering sequence.
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My Notes:
This home plan could save you an average of $1,073 per year in energy costs »
From the south: Head northwest on FL-417 Toll N toward Exit 30. Take Exit 30 for Curry Ford Rd. Turn right on Curry Ford Rd. The community will be on the left.
From the north: Head south on FL-417 Toll S toward Exit 30. Take Exit 30 for Curry Ford Rd. Turn left on Curry Ford. Rd. The community will be on the left.
If using a GPS device please input 10026 Curry Ford Rd. Orlando, FL 32825, the community will be directly northwest
The Santa Maria at Econ Landing in Orlando is a thoughtfully designed town home floor plan featuring 3 bedrooms, 2.5 bathrooms and a 1-car garage with 2-car wide paver driveway. The 1st floor of this home features an open concept Great Room/Kitchen combination with a powder bath and large paver lanai. The 2nd floor features additional living space at the Loft, a large Master Bedroom retreat with a huge walk in close and Luxury Master Bath Suite featuring dual sinks, a garden bathtub and separate shower. 2 additional bedrooms are located to the rear of the home and share a full bath. The washer and dryer hook up is located on the 2nd floor too which is very convenient. No lugging the laundry upstairs! Best of all this home is 100% Energy Star 3.1 certifies which means it is affordable to operate and you will save money on your energy bills. Some of the energy saving features include Core Fill insulation in the block walls, R-38 ceiling insulation, Radiant Roof Barrier, Low-E double pane windows and Energy Star appliances.


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Almond Chocolate Biscotti (Using Cake Mix)
Recipe by mary winecoff
This is a quick biscotti recipe that starts off with a chocolate cake mix. Very quick and easy and perfect for after dinner drinks or coffee.
Top Review by Chef PotPie
YUM! I had to divvy these up between DH for at work, DD and fiance, and myself because everybody loved them. I used a devil's food mix, (I LOVE the simpliticy of using a mix), and when I cut them they were a bit wider than 1/2 inch. (I had some cracking in the "logs" and I found the trick to cutting them without causing crumbling was to lightly start the cutting through the very top crust with the serrated knife, then push straight down to cleanly cut.) They came out of the oven the second time with just a little semi-softness in the centers, like a dense brownie texture, YUM!, and a crusty outside. I spaced my white chocolate on the stove and it siezed up, so I had to smear it on...sorry for the photo, but they sure were delicious! I will make these again. ZWT 7 - Shady Ladies
See Discussion:6 Reviews
Ingredients Nutrition
- 1 (18 ounce) package chocolate cake mix
- 1 cup all-purpose flour
- 1⁄2 cup butter, melted
- 1⁄4 cup chocolate syrup
- 2 eggs
- 1 teaspoon vanilla extract
- 1⁄2 teaspoon almond extract
- 1⁄2 cup almonds
- 1⁄2 cup miniature semisweet chocolate chips
- 1 (12 ounce) package vanilla or 1 (12 ounce) package white chocolate chips
- 2 tablespoons shortening
Directions See How It's Made
- In a large mixing bowl, combine the cake mix, flour, butter, chocolate syrup, eggs and extracts; mix well.
- Stir in the almonds and miniature chocolate chips.
- Divide dough by half.
- On ungreased baking sheets, shape each portion into a 12 x 2 log.
- Bake at 350F for 30-35 minutes or until firm to the touch.
- Cool for 15 minutes.
- Transfer to a cutting board; carefully cut diagonally with a serrated knife into 12 inch pieces.
- Place cut side down on baking sheets.
- Bake for 10 to 15 minutes or until firm.
- In a heavy saucepan over how heat, melt vanilla chips and shortening.
- Drizzle over biscotti; let stand until hardened.
- Store in airtight container.


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Event: Bollywood celebs gathered for the 'Chautha Ceremony' of late Shammi Kapoor.
Guest List: Imran Khan, Lara Dutta, Tabu, Prateik Babbar, Chunky Pandey, Jeetendra, Vinod Khanna, Prem Chopra, Randhir Kapoor, Aditya Raj Kapoor, Mukesh Khanna, Rishi Kapoor, Naseeruddin Shah, Ratna Pathak Shah, Jagmohan Mundhra
Date: 16-Aug-2011
Tiger Shroff's family Jackie Shroff, Ayesha Shroff, Krishna Shroff along with his co-star Nidhhi Agrewal, director Sabbir Khan were present at the special screening of MUNNA MICHAEL. Danny Denzongpa w...
Anushka Sharma, director Imtiaz Ali launched JAB HARRY MET SEJAL first trailer sans Shah Rukh Khan
Kareena Kapoor Khan, Soha ALi Khan, newbies Aadar Jain and Anya Singh were spotted in Bandra. While Kareena pouted for photographers in her gym wear, Aadar Jain and Anya Singh went together for workou...
Bollywood's hot sensation Disha Patani was spotted airport in casuals
Bollywood actor Shahid Kapoor spotted at airport


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D Cup
Categories: Big Tits, Public
Mikayla Mendez and AJ Baily tongue pummel eachother.
AJ and Mikayla can't even wait to leave a public place before they assault eachother! They penetrate eachother with thier munches and playthings right ontop of the bar!!525 Views
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Wedding photography, wedding photographer, chester , north wales, north west ,wirral.
Welcome to Stephen C. Jebb Photography.
I am a wedding photographer covering North Wales, Chester and the North West.
As a photographer I strive to produce beautiful photos that you will love. I can also create stunning images of any new addition to your family, be that two or four legged.
If you are getting married, expecting your first baby or have a newborn, have a new dog or puppy, a life long furry companion or just love your horse, then click on the tabs above to see what I can offer.
I look forward to hearing from you and hopefully will be meeting soon to take some great photos for you.
Chester dog photography,Baby photographer, children photographer, horse photographer, chester photographer, pets, pet portraits, baby portraits, equine photographer Chester, horse photographer, Chester photographer, Cheshire photographer, county, photos, albums, events, stables, hacking, horse, horses, babies, baby, bumps, maternity, new born, Cheshire, children, studio, outdoors, parks, forrest, river dee, Grosvenor, eaton, puppy, puppies, family, kids, canine, churton, the white horse,
© 2017 Stephen C. Jebb Photography.


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Mathnook Offers Games Aligned with Common Core Standards
According to the Mathnook staff, it's all part of a larger plan to provide entertaining, educational math materials to home schoolers, teachers, and students seeking to improve math skills with fun and challenging activities. "Mathnook believes math games, worksheets and other teaching tools don't have to be boring to be helpful! We specialize in the kinds of activities kids love, while building skills at the same time," says a company spokesperson.
As the Common Core organization'
Parents, students and educators may search for activities on Mathnook.com based upon the skill they seek to acquire. "Games like Math Balloon Integers and Golden Zeros Challenge, Baby Budget and Bus Driver Math are designed to allow students to learn and reinforce math strategies while having fun in the process," the company spokesperson explains. With Common Core Standards materials available from kindergarten through high school, students outside traditional classroom settings can access Mathnook materials quickly and easily to participate.
One mother, Karen J. of Norfolk, Virginia, asserts that the standardized games and tools offered by Mathnook answer a real need in the homeschooling community for high quality educational resources. "I'm always looking for new ways to challenge my kids, but sometimes it's hard to find advanced math teaching materials that they can relate to," she says. "We were really happy to find Mathnook and see all they had to offer."
Mathnook reports that their website is seeing increased traffic numbers on a daily basis, and more calls and email inquiries about their Common Core aligned games and activities. The company's newfound popularity suggests the importance of their materials for academic success outside the bounds of the typical classroom.
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Fans aren't the only ones who have all sorts of ideas for way the characters from Doctor Who could appear in their own spin-offs. John Barrowman proposed his idea for a spinoff starring Captain Jack and River Song during SDCC. Speaking with theTVaddict he noted:
"I'd like to see the two of them prior to their lives crossing in some time realm where they actually meet up without the Doctors or anything like that and they are rogue heroes. Kind of like what Star Trek did in the very beginning, going around and saving civilisations without trying to get too involved, but they always get involved! They always do the wrong thing but for the right reasons and succeed."
And given that it's Captain Jack we're talking about, Barrowman has ideas for how romance could bloom.
"I think there would be a lot of standing off between the two of them to begin with, because they are two very, very strong characters. Very flirtatious! There would be a lot of innuendo going on. I think they would not do anything with each other for a long time because it would be too predictable. They have to be confined in a spaceship for a little while."
Of course, this will never be made and we will never get to see it happen in any way possible ever, but it's nice to dream.
(via blastr)


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Tourist Directory > Hotels
Sleepy Sams
Address:54 (2nd Storey), 55 & 56 Bussorah Street, Singapore 199471
Contact:(+65) 9277 4988
Description: Formerly the grounds of the Sultan's palace, this boutique-styled hostel now resides in this area and is well furnished. It is located just down the road from the Sultan Mosque and Arab Street, which are areas to learn about the local Malay culture.
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Tydlacka Named SEC Special Teams Player of the Week
Nov. 28, 2011
LEXINGTON, Ky. – University of Kentucky senior punter Ryan Tydlacka has been named the Southeastern Conference Special Teams Player of the Week for his solid performance in Kentucky's historic win against Tennessee last Saturday, the league office announced Monday.
Tydlacka was a vital part of Kentucky's success last Saturday, flipping field position the entire game to help keep the UT offense backed up. The senior from Louisville, Ky., punted nine times Saturday for an average of 43.6 yards. His excellent punt placement and hangtime did now allow Tennessee to return a punt the entire game. Tydlacka's efforts included a 60-yard punt and Tennessee did not begin a drive outside its own 30-yard line during the entire game.
The senior finishes the season with 79 punts for an average of 43.6 yards and 19 punts inside the other team's 20-yard line. Tydlacka ranks second in the SEC and 17th in the nation in punting, while Kentucky ranks second in the conference and 13th in the nation in net punting.
Tydlacka was named Mid-Season All-America honorable mention and Mid-Season All-SEC third team by Phil Steele's College Football, while being named honorable mention punter of the week by the College Football Performance Awards a total of five times this season.