text:
The State of Manufacturing & Innovation
MBDA Business Center- Atlanta
Tuesday, July 15, 2014 from 2:00 PM to 4:00 PM (EDT)
Who's Going
San Francisco, California
London, United Kingdom
Florence, Italy
Event Details
The MBDA Business Center-Atlanta invites you to The State of Manufacturing & Innovation featuring Dr. Ben Wang of the Georgia Institute of Technology Manufacturing Institute (GTMI) and Alejandra Y. Castillo, National Director of the MBDA.
Join a community of manufacturers, MBDA Business Centers, strategic partners, researchers and innovators at Georgia for the national launch of the Center's advanced manufacturing initiative, Connecting Advanced Manufacturing Program (CAMP). This interactive webinar will include an introduction by Castillo, who will discuss MBDA's focus and commitment to Minority Business Enterprise (MBE) manufacturers and President Obama's commitment to manufacturing and exporting. Additionally, Dr. Wang will present a dynamic synopsis on 3D printing, biomanufacturing and composites.
When & Where
Georgia Institute of Technology
75 5th St NW
Hodges Room, Suite 335
Tuesday, July 15, 2014 from 2:00 PM to 4:00 PM (EDT)
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Reallusion CrazyTalk Animator 3.03.1230.1
Reallusion CrazyTalk Animator 3.03.1230.1 Pipeline
What's New in CrazyTalk Animator 3:
Production Environment
• 3D camera system.
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Create 2D Characters
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• Create multi-angled 2D characters.
Animate 2D Characters
• Animate 2D characters with 3D motions.
• Elastic and bouncy curve editing.
• Facial puppet and audio lip-syncing.
• 2D facial Free Form Deformation.
Create 2D Talking Heads
• Sprite-based Facial System.
• Morph animation from facial images.
• Employ talking heads on any character.
Prop Dynamics & SFX
• Give life to props - Elastic Motion.
• Create Interactive Compound Animations.
• Combine props & link SFX.
• Light and shades - Effect Layers
Import & Export
• Immerse your video actor into a 2D animation.
• Supports popular image and video formats.
• Image sequence export for video compositing.
Pipeline Features
• Import 3D motions for 2D character animations.
• Custom create new character bases.
• Import Photoshop PSD layers for character creations.
• Create unique Elastic Motion libraries.
• Design vector-based content with Multiple Render Styles.
Upload GIG
Don't forget to say Thanks !!!
Reallusion CrazyTalk Animator 3.03.1230.1
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subset: commoncrawl


text:
1.61 F.B. Pant
1.61 F.B. Pant
1.61 offers heirloom apparel androgynous in nature with a unisex fit. The F.B. Pant offers the comfort of cotton and the shaping of raw denim. Hook and eye closure with zip fly. Side angled pockets. Made in U.S.A. Pants run true to size. Ashley is wearing a Size 25.
Shipping and returns (when eligible) are FREE for purchases from US and Canadian boutiques when shipped to customers located in the US or Canada. We offer a flat shipping fee of $45 for all international transactions.
Shipping Details
Returns are accepted on full-priced items except when item is marked as Final Sale. All sale items, including Make an Offer items, are FINAL SALE.
Returns Policy
Baby & Company
Since 1976, Baby & Company has lived by the mantra: life is a marathon not a sprint. The Seattle-based boutique is a proud member of the Slow Fashion movement where quality comes before quantity. The shop stocks up on its values with like-minded brands like Jesse Kamm, Ann Demeulemeester, Antoni & Alison, Lars Andersson and more. Clean lines and the simple, easy aesthetic found on their racks echo the 'less is often more' spirit of the brand.
Do you have questions about this product? We're here to help!


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The Jinjiang Zhixing Si Chuan Bei Lu is a Tourist hotel. Located in SHANGHAI area. Shopping is accessible by bus/taxi and the nightlife/restaurants are easily accessible by taxi or bus from the hotel.
Located at No.440 East Baoxing Road. Jinjiang Inn, Shanghai North Sichuan Road Inn, is only 50 meters away from East Baoxing Road station of the Pearl Light Rail. The Inn is in the vicinity of North Sichuan Road shopping mall and the Duolun Famous Culture Person Street, characteristic of well developed transportation, prosperous commerce and deep cultural atmosphere.


text:
About the Book
I love photographs that capture a transitory, low key moment in time when the relationship between the environment, the subject and the instant combine in a way that strikes a chord with me and will never exist again. I am fascinated by how we relate to our environment and how the viewer of the photograph connects with the photographer who connects with the subject of the image.
Features & Details
- Category Fine Art Photography
Large Format Landscape, 13×11 in, 33×28 cm
78 Pages
- Publish Date May 22, 2008
- Tags black and white, New York, intimate moments, travel, street, rangefinder, people, London, Cornwall, England
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TORONTO TO PARIS, FRANCE FOR $372/500CAD R/T (summer 2016)
Amazing deal: Toronto to Paris, France flights, roundtrip as low as $372/500CAD.
Toronto, Canada
Paris, France
Toronto, Canada
AVAILABILITY: limited
Departures: May 23, 30
Returns: May 25, 27, 29, 30, June 1, 3, 6, 8, 15
We do not sell travel products or services. We just provide you with information (how/where) about flight deals or links to websites, where you can read more about it.
Information is valid at time of publication. Due to a high amount of posts – it is hard to track down all expired offers, so sometimes you may not find the price mentioned in the post. It means that the deal was sold out or supplier (airlines/agency) has changed the conditions or removed the offer. To avoid these frustrating situations, you can subscribe to our newsletter, Facebook, Twitter, Pinterest, Tumbler.
Image Credits:image copyrights Moyan Brenn used under CC BY 2.0 license / resized from original .


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Students of Dining studies need not worry about paying for over-priced textbooks anymore – renting textbooks is an option! Dining textbooks – for example Time Out Miami 1 (Time Out Guides) by [Time Out], and Time Out Los Angeles 2 (2nd ed) by [Time Out] – are often some of the most frequently updated books by Publishing companies. The field of Dining is constantly changing, and new editions can come yearly and at a huge cost to students who continually buy new books. So why buy when you can rent cheap textbooks? There are plenty of Dining books to rent – BookRenter has of them all!
Used book sales can be a cheap option as well, but often can be more expensive than textbook rentals. However, there are very few return policies with used books, and especially when taking Dining classes, buying a used book can be dangerous and costly.
List Price:$14.95
List Price:$14.95
List Price:$24.95
List Price:$17.99
Nyassa; a Journal of Adventures whilst Exploring Lake Nyassa, Central Africa, and Establishing the Settlement of 'Livingstonia': Revised by Rev. Horace Waller. With MapsList Price:$16.99
Denver Omelet: A Guide to Breakfast and Brunch Dining in the Denver Metro Area Including Boulder, Lafayette, Louisville, and NiwotRentList Price:$13.95
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Watch: Orioles OF Travis Snider makes full-extension diving catch
Baltimore Orioles outfielder Travis Snider made an impressive diving catch to rob Cleveland Indians shortstop Francisco Lindor of an extra-base hit in Sunday's game.
With the Orioles leading 8–0 in the top of the sixth inning, Lindor hit a long line drive to left centerfield. Snider tracked the ball into the gap and dived to snare it, robbing Lindor of at least a double.
The ball left the bat at 98 mph and traveled 336 feet, according to MLB's batted ball data.
Snider also had a nice day at the plate. He hit a solo home run in the fourth inning and scored two runs.
The Orioles won the game 8–0 and swept the doubleheader.
• Mets pitcher Steven Matz impresses at the plate in debut
- Dan Gartland


text: SeaCube Container Leasing Ltd. (SeaCube) (NYSE: BOX), one of the world's largest lessors of intermodal freight containers, today announced that the Company's Chief Operating and Chief Financial Officer, Steve Bishop, is scheduled to present at the Deutsche Bank Securities, Inc. 2012 Global Industrials & Basic Materials Conference in Chicago, IL on Thursday, June 14, 2012 at 8:40 a.m. CT (7:40 a.m. ET). The presentation will be broadcast live over the Internet and can be accessed on the Investors section of the Company's website at www.seacubecontainers.com. Please allow extra time prior to the presentation to visit the site and download the necessary software required to listen to the internet broadcast. A replay of the presentation will also be available on the company's website for 90 days following the live event. About SeaCube SeaCube Container Leasing Ltd. is one of the world's largest container leasing companies based on total assets. Containers are the primary means by which products are shipped internationally because they facilitate the secure and efficient movement of goods via multiple transportation modes, including ships, rail and trucks. The principal activities of our business include the acquisition, leasing, re-leasing and subsequent sale of refrigerated and dry containers and generator sets. We lease our containers primarily under long-term contracts to a diverse group of the world's leading shipping lines. For more information regarding SeaCube Container Leasing Ltd. please visit www.seacubecontainers.com.


text: Frankie Bones - My House is Your House (L-Train Remix) by DJ L-TRAiN published on 2013-02-28T17:29:13Z --------------------------- www.facebook.com/ltrainmusic =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=- People have been asking me about this tune, so here are some details about it: The only part of the track that has any parts or qualities related to the Frankie Bones original from 1990 is the vocal "My house is your house, and your house is mine". Everything else was produced and arranged by me, aside from the well known synth sample from "Art of Noise - Moments in Love". It's a full Remix / Remake and not a ReRub. Hope you all enjoy and thanks for listening! Genre Breakbeat Comment by TheDjChorlo TEMAZOOOO 2014/10/24 19:04:20 +0000 Comment by Da Beat Wobbler mate supa shit dude :D 2014/09/11 11:59:51 +0000 Comment by sweet_girl_in_the_313 love it! 2013/06/06 14:12:52 +0000 Comment by Christina Paradyse Bad ass! 2013/05/31 23:27:12 +0000 Comment by afcdave123 Oh yes 2013/04/14 09:33:49 +0000 Comment by Bit 2 Beat nice work 2013/03/09 16:09:15 +0000 Comment by (double)Agent 137 Great sample. It's the quiet storm... then BOOM! 2013/03/09 03:37:29 +0000 Comment by Thee Nikki Bri L Train in da house, dope as always!!! 2013/03/07 04:26:46 +0000 Comment by Dusty Tonez nice 2013/03/05 12:35:11 +0000 Comment by MichelleMoore F yeah! One day there was Jack.... and Jack had a groove. And from this groove came the groove of all grooves.. Let there be house! This song is lacking that. But the Art of noise stuff 2013/03/05 08:50:38 +0000 Comment by Manki / Melotonin https://www.facebook.com/BreakbeatRomania (published) 2013/03/04 11:43:16 +0000 Comment by Break'a Bot Mazor Promo Dope! 2013/03/02 22:36:48 +0000 Comment by Break'a Bot Mazor Promo PHATNESS! 2013/03/02 22:36:25 +0000 Comment by Break'a Bot Mazor Promo One of my fave ol school tracks! 2013/03/02 22:35:06 +0000 Comment by crazy dream 2.1.0 BUEN REMIX BROO ; ) 2013/03/02 22:27:26 +0000 Comment by Frankie Bones The whole concept of "My House Is Your House" was taking one great moment and turning it into another. So to take a moment which is 23 years old, A moment of love, if you will? And to breathe new life into it has to be taken with extreme care. I suppose L-Train realized this in advance. There is actual substance here, the reason remixes are created in the first place. I'll personally support this as an official remix and let L-Train have his moment, as he should. 2013/03/02 16:35:56 +0000 Comment by ReneeBrillant Hell yes! 2013/03/01 17:06:58 +0000 Comment by LIMEe INTERNATIONAL/RECORD PRODUCER/DJ/D2R ILL 2013/03/01 17:03:42 +0000 Comment by -cant-get-enough- helllll yeaaaaaaa, some old Frankie Bones 2013/03/01 04:06:57 +0000 Comment by Sam Groove Big! 2013/03/01 01:25:07 +0000 Comment by Nasty Cash Nice... 2013/02/28 23:37:29 +0000 Comment by Nasty Cash I like that Moments in Love... 2013/02/28 23:37:18 +0000 Comment by Mista Maxx sick track! thanks for the dl! 2013/02/28 22:59:27 +0000 Comment by Sharted SICK! 2013/02/28 22:04:23 +0000 Comment by Low"Beat Boommmmmmmmm!!!! THX friend = D 2013/02/28 21:15:57 +0000 Comment by X- STATIC excellent remix!!! 2013/02/28 19:47:59 +0000 Comment by EXZILE Classic Funk! 2013/02/28 18:42:15 +0000 Comment by HICUE great track meng! 2013/02/28 18:30:51 +0000 Comment by HICUE nice! 2013/02/28 18:30:04 +0000


text:
About the Book
Gary and Julie Lawrence's travels across the north and west of Australia were recorded in words and photographs on their blog Passing~Time. Excerpts are printed in this beautiful travel journal
Features & Details
- Category Travel
Standard Portrait, 8×10 in, 20×25 cm
150 Pages
- Publish Date Nov 13, 2010
- Tags Northern Territory, Western Australia, South Australia, Australia, travel, caravan, photography, Nikon, NSW, Queensland
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About the Creator
Gary Lawrence
Bangor, NSW, Australia
Retired Head Teacher of Science and teacher of Computing, Photography and Multi Media. Experienced photographer with interest in travel, sports and candid people photography. Volunteer Photomarshall at Sydney 2000 Olympic Games, World Cup Rugby Sydney 2003 and World Masters games, Sydney 2009. Lifeline volunteer telephone crisis counsellor
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By Dana Hunsinger, The Indianapolis Star
INDIANAPOLIS Rapunzel with her tangled hair is getting VIP treatment on toy store shelves Friday.
Nike's Air Jordan Retro 13 basketball shoe is splashed in ads for The Finish Line.
And a Maytag stainless steel refrigerator is all the rage at hhgregg.
But why?
BLACK FRIDAY: Tips, deals and more
BEYOND BLACK FRIDAY: L.L. Bean, etc., have high hopes
SHOPPER PANEL: Are you ready for the holiday season?
Ever wonder how merchandise ends up on shelves for Black Friday, the traditional start of the holiday shopping season and one of the busiest of the year? Ever wonder why the doorbuster deals are the doorbuster deals?
Well, you may be buying it Friday, but a product's journey to land a prime spot on Black Friday is a long and complicated process that started months and, in some cases, more than a year ago.
"Let's put it this way. We are looking at Black Friday 2011 right now," said David Stickney, merchandise manager for toys at Meijer. "We don't make these decisions in a vacuum. We take a lot of data, a lot of research and we put it into play. Like who would have thought that Rapunzel would have had any kind of a chance to survive nowadays? But she absolutely does."
She does because of "Tangled," a movie all about the long-haired, fairy tale gal that hit theaters Wednesday.
Movies are something toy buyers pay a lot of attention to. But there are all sorts of other indicators and trends that all types of buyers watch for before deciding what to purchase for Black Friday.
The Indianapolis Star talked to three buyers to find out how all that stuff you are going to buy Friday ended up on store shelves.
Footwear buyer
Jeff Morrell began calling in orders for Black Friday shoes in May. That's because it always takes six months for delivery of athletic shoes.
He and his team of three buyers and one associate look at everything from what pro athletes are wearing to what physical activity is popular. They look at what's been selling well all year long and what will launch close to Black Friday.
If Morrell hears a shoe manufacturer is going to budget big to advertise a certain sneaker, well, that shoe makes sense to have in stock.
Then, he and his team sit back and wait to see if what they bought sells.
"It's so much fun to see something come from inception to six months later when it finally comes to the floor and then it sells," said Morrell, vice president of footwear for the Indianapolis-based company. "You have to be a very good predictor to be a buyer."
But it's not all about predictions.
Finish Line buyers spend a lot of time traveling to stores across the country. They tote shoes with them, set up a table and ask customers what they like and don't like.
"We have to be in touch with the consumers every day," Morrell said.
Beyond that, Finish Line looks at sales data. After all, what people like on the West Coast is quite different from the East Coast. And the size of shoes people wear in the Midwest (an average foot size) is different from the shoe sizes of those on the West Coast (small feet).
But overall, the styles and what is really hot remain steady from store to store.
So what's really hot this year?
"It's all hot," said Morrell. "Are you kidding? We wouldn't buy it if it wasn't hot."
OK. He reluctantly revealed a few of the hotter items. There is the Adidas MEGA Soft Cell, a basketball shoe promoted by rapper B.o.B. and a Finish Line exclusive. The Reebok Zig Pulse running shoe. The Nike LunarMax running shoe
And then there is the smoking hot Nike Air Jordan Retro 13, which was an easy shoe to decide to carry for Black Friday. That's the day it launches.
"They've found that's a pretty good formula for them," said Morrell, adding he knows it will sell out at all his stores.
So wouldn't a smart buyer buy more?
"That's allocated by Nike," Morell said smiling. "Their intent is to keep demand higher than supply."
That keeps customers wanting more and you better believe Morrell will be buying.
Toy buyer
Who knew the teenaged children of famous monsters would be every little girl's dream this Christmas?
David Stickney did before Christmas 2009 had even hit.
As all you shoppers fight over Zhu Zhu pets and Squinkies, the merchandise manager for toys at Meijer is gearing up to order Christmas 2011 toys next week. All of next year's Black Friday toys will be ordered by February.
But that doesn't stop Stickney from being able to rattle off the popular toys for this holiday season.
Monster High fashion dolls like Frankie Stein and Draculaura. Squinkies. Beyblades. Zhu Zhu pets. "Toy Story 3" and "Tangled" movie toys. Paper Jamz guitars. Lego board games. Matchbox Stinky the Garbage Truck
PHOTO GALLERY: Mattel toys
PHOTO GALLERY: Hot toys for 2010
To figure out what made sense this year, Stickney and his team of four buyers scoured the industry to find the hot licensed products. They looked at kids' movies and the expected box office sales. They read over pre-toy testing results conducted by manufacturers.
In other words, what did the actual kids think when they got the toy in their hands?
"At the end of the day, cool toys are only cool toys if they are fun," he said.
When it comes to product buying, it may be Stickney's job that is the most crucial.
"About 60% of the entire toy industry happens in the fourth quarter," he said. "Sure you have Easter and birthday parties and the 'You are a good kid' kind of day. But the wow happens during the holidays."
So if a product flops, it can be a big loss, and Stickney admits that has happened on occasion.
"Toy buying is even more ridiculous than the fashion department because it's that fickle," he said. "You have to find something that can not only capture kids' imagination and keep them occupied but also draw their attention because there are a lot of competing products out there."
Electronics/appliance buyer
A dishwasher for Christmas? Not too sexy. But it's going to be happening in homes across the country.
"Appliances are extremely hot," said Gregg Throgmartin, executive vice president and chief operating officer for hhgregg. "There has been built up demand and people are tired of that leaky washer or that dishwasher on the fritz."
So hhgregg bought up plenty of appliances for Black Friday because manufacturers were offering some great deals.
There is a Samsung Front Load washer for $499 and a Maytag stainless steel refrigerator with all the bells and whistles for $999.
But as always, electronics are a major mover on Black Friday as well.
To decide what to carry is a pretty simple formula in his industry, Throgmartin said. And it should start a year in advance, he said.
"To be honest, it really should start the Saturday morning after Black Friday," he said. "A good retailer should be making all of your notes the next day while it's all fresh. While prices may change for the following year, you get a feel for price points that were hot. Things people gravitated toward, didn't gravitate toward."
Beyond that, hhgregg tracks sales daily, weekly and monthly.
"You start to see industry trends," he said.
This year, there is excitement around 3-D. This year, big TVs are going for cheap. At hhgregg, there will be a $9.99 DVD player and a 24-inch HDTV for $149. There is a $29 camera camcorder.
About 40% of purchases, however, will be for the shoppers themselves and not others.
"That 3-D TV doesn't necessarily end up under the Christmas tree, but it is the gift for the family or for ourselves," he said.
Throgmartin said his company was still making Black Friday purchases just weeks ago.
"You print your ads ahead of time and then there are last minute specials by manufacturers," he said. "They will say, 'If you like $599 TV with LG (color), how would you like a $499 Samsung with LG? That's where retailers like ourselves can be quick and really excel. We just buy it at the last minute."
Guidelines: You share in the USA TODAY community, so please keep your comments smart and civil. Don't attack other readers personally, and keep your language decent. Use the "Report Abuse" button to make a difference. Read more.


text:
Your answer is one click away!
In my system, I would like to intercept and change Cypher queries as they come in, one alternative is to modify them before sending them from my middle layer to the graph - but is there a way to have a plugin do the conversion for me in the graph itself?
I'd like to do some of the following: If someone identifying themselves as members of group A, imagine I'd like to change their request from:
MATCH(f:Film)-[r:REVIEWED_BY]-(u:User {id:"1337"})
MATCH(p:Product)-[p:PURCHASED_BY]-(u:User {id:"1337"})
Is something like this possible? Or do I have to write the traversals in Java directly to achieve this?
At this time it's not possible to extend or modify Cypher queries.
If you need that I recommend you to use Transaction Event API - http://graphaware.com/neo4j/transactions/2014/07/11/neo4j-transaction-event-api.html
With that you should be able to change what query returns.
Of course you can. You can do ANYTHING in Neo4j. Just grab the cypher string in an unmanaged extension that receives a post request, alter it any way you want, execute it with the graphdb.execute method and return the result as normal.
public Response alterCypher(String body, @Context GraphDatabaseService db) throws IOException, InterruptedException {
ArrayList<Result> results = new ArrayList<>();
// Validate our input or exit right away
HashMap input = Validators.getValidCypherStatements(body);
ArrayList<HashMap> statements = (ArrayList<HashMap>)input.get("statements");
for (HashMap statement : statements) {
// write the alterQuery method to change the queries.
String alteredQuery = alterQuery((String)statement.get("statement"));
Result result = db.execute(alteredQuery, (Map)statement.getOrDefault("parameters", new HashMap<>()));
// or go the results and return them however you want
// see https://github.com/dmontag/neo4j-unmanaged-extension-template/blob/master/src/main/java/org/neo4j/example/unmanagedextension/MyService.java#L36
return Response.ok().build();


text: Portage city Council Portage Council Sets Meeting on Appointment of Acting City Manager By Sehvilla Mann • Mar 11, 2014 Sehvilla Mann / WMUK The Portage City Council will hold a special meeting next week to consider the appointment of an acting city manager. Current Portage manager Maurice Evans will retire on April first, and by law the city must appoint a successor within 30 days. Mayor Pete Strazdas has recommended the appointment of current deputy city manager Brian Bowling. Several council members said they need more time to consider the proposal. Portage City Council Reinstates Senior Citizen Discount on Water Bills By Gordon Evans • Feb 12, 2014 The discount had been in place for more than 30 years and was ended in October Portage Council Considers Next Steps in City Manager Search By Sehvilla Mann • Feb 11, 2014 Sehvilla Mann / WMUK Portage City Council members say they hope to decide soon how they'll select the city's next manager. Maurice Evans announced his retirement on Monday after eight years at the post. At the city council's meeting yesterday, Mayor Pete Strazdas suggested councilors weigh different approaches with the hope of approving one at their next meeting. He says the first step is for members to decide what "qualities" they're looking for in Evans' successor. Considerations include whether to hire a search firm to find candidates and a consultant to assist with decision-making. Portage to state: don't mess with our chickens! By Andrew Robins • Jan 22, 2014 The ordinance allows Portage residents to keep up to six chickens as long as certain conditions are met. Portage backs transit planning, with reservations By Andrew Robins • Jan 22, 2014 The Kalamazoo County Transportation Authority hopes to have the regional bus plan ready next year. Pages« first ‹ previous … 3 4 5 6 7 8 9 10 11 next › last »


text:
Alyssa Zamora from the Duke Global Health Institute visited the Baby Monitor team recently in Bungoma East District during a meeting with a great group of community health volunteers who are helping to design Baby Monitor's referral/dispatch system. Read her post here.
The group of Kenyan community health care workers met with Green, Master of Science in Global Health student Amogh Karnik (read his blog) and undergraduate Mark Herzog at the Sinoko Dispensary to collectively brainstorm how they might use technology and a new referral and tracking system to track pregnant women and connect them with care.


text:
Get flat ₹ 100 off on your first buy.
Your Voucher Will Appear Here!
Loading, please wait...
Cash on Delivery for orders above ₹ 1,500 and below ₹ 10,000.
0 products to choose from
Today, in India, RMCL is the largest manufacturer and exporter of cleaning equipment. A state of the art manufacturing facility and a comprehensive marketing and After Sales Service network, enabled the company to deliver optimum solutions for customer's cleaning needs. Products from RMCL are built around eco friendly concepts and comply with international quality and safety norms
No products for this manufacturer.


text:
reaction points
This is probably one of the dumbest questions you could be asked, Kool so I apologize ahead of time. Is there anyway to know when price action reaches the 161 or 261 and you get a "reaction", whether it is just a short-lived retracement before a resumption of the main trend, or whether its the beginning of a new trend in the opposite direction?
Something I have noted. When a bar closes above the 1.618 projection and it pulls back if crosses back above the 1.618 it usually runs up to the 2.618.
correct DOESONE ..We have noted that before... also be aware that when a 2.618 is hit a a reaction occurs , it generally travels back to the 1.618! (unless there is a sizable overshoot or undershoot)
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text:
JavaOne Session "Lean and Opinionated JavaEE Applications" Is Available
My (Adam Bien) JavaOne talk: "Lean and Opinionated Java EE 7 Applications" (CON2196) is available online:
"...Java EE encourages a lean development approach without any ceremony..."
See also other screencasts at: http://tv.adam-bien.com or subscribe to http://www.youtube.com/user/bienadam.
See you at Java EE Workshops at MUC Airport, particularly at the Java EE Architectures workshop!
NEW workshop: HTML 5 and JavaScript Essentials, Three days in April 2017: from Java EE 7 Architectures over Microservices to Performance, Troubleshooting and Monitoring
On demand workshops: Java EE 7: Bootstrap, Effective, Testing and Microservices available for streaming.
Newsletter: airhacks.news


text:
Bill Waters was going through an antique store when he discovered a yellowing ledger full of formulas. What early research had he stumbled upon, he wondered? Would he learn about the origins of man's fight against disease, or our first attempts to tame the atom? Better: he believes that he has in his possession the original drug-store formula for delicious Dr Pepper.
via Yahoo! News


text:
Need a Thyroid Test in Kalispell, MT? Simply order online 24/7 and get a fast, affordable Thyroid Test at a lab near you (Find More Locations):
What is a Thyroid Test?
A Thyroid Test consists of lab tests to check the thyroid. People in Kalispell, MT get a Thyroid Test for general screening or at the request of a medical provider.
How much does a Thyroid Test cost?
Our Thyroid Test prices include the doctor's order that you need to get tested in Kalispell, MT, all lab fees and taxes, and a pdf copy of your results. For current prices, please CLICK HERE.
What does a Thyroid Test lab report look like?
For a popular Thyroid Test sample report, please CLICK HERE.
We are delighted to offer the Thyroid Test in Kalispell, MT.
Thyroid Test in other cities near Kalispell, MT:Bigfork | Columbia Falls | Coram | Dayton | Hungry Horse | Kila | Lake Mc Donald | Lakeside | Martin City | Proctor | Rollins | Somers | West Glacier | Whitefish


text:
Dr. Eva Håkansson is setting world records with KillaJoule, her electric motorcycle which was custom-built with help from 3D printers.
Don't Miss: 35 Most Influential Women in 3D Printing
Eva Håkansson has sped onto the scene of electric vehicles and hopes to challenge two main stereotypes. She's already proving that women can be engineers, and electric vehicles can be fast.
Right now, her electric motorcycle KillaJoule holds the record of 248.746 mph or 400.2 kph. With more 3D printed developments in the pipeline, the electric vehicle could become even faster yet.
"The real purpose of the KillaJoule is what I call eco-activism in disguise," Håkansson said. "We want to change the general public opinion about electric vehicles and particularly changing the image that they're slow … by building something that is so fast that nobody can ignore it."
Håkansson received her masters and PhD in mechanical engineering from the University of Denver, USA. You can listen to her give insight into her project and her experiences building KillaJoule in the video below:
The Role of 3D Printing in KillaJoule
For five days every year, Håkansson races KillaJoule in Bonneville Salt Flats, Utah. It's here that she's able to show off the work she put in every day for the previous 360 days.
Håkansson sings the praises of 3D printing and everything it can do for speed. She said: "There is no more wonderful, beautiful way of creating aerodynamic parts like spoilers, leading edges, and so on than 3D printing. 3D printing is the absolute optimal way of doing that."
Predominantly, Håkansson used 3D printing for KillaJoule's speedometer housing. Using a Lulzbot 3D printer, she found INOVA-1800 was the best material for her needs. For the sidecar, she used biodegradable PLA to create aerodynamic shapes.
"If you compare to what it costs to have parts made or the time you would spend machining something similar or building it with using other methods, 3D printed parts are super cheap," Håkansson said.
It's no surprise then that Håkansson plans to use 3D printing in her next project – a new bike. This bike could feature even more 3D printed parts. If you're interested in keeping up to date with Håkansson's work, make sure to visit her website.
We certainly agree with Håkansson's stance on 3D printing. She continued: "It's opened a whole new dimension of manufacturing. You can do things you can't even dream of making otherwise."
Source: Lulzbot
License: The text of "KillaJoule: Record (and Stereotype) Breaking Electric Motorcycle" by All3DP is licensed under a Creative Commons Attribution 4.0 International License.


text:
If you like great ideas, great writing and stimulating discussions, please join us. The group gives people the opportunity to discover, discuss and learn from outstanding works of writing.
Every month we read and discuss a short selection by famous authors such as Aristotle, Sigmund Freud, Alexis de Tocqueville, and Virginia Woolf.
Meets every third Tuesday of the month. For information, contact Nancy: email@example.com or (415)566-1392. Co-sponsored by the non-profit Great Books Council of San Francisco and the Sunset Branch of San Francisco Public Library.
Upcoming Events
- Homegoing
- Tuesday, July 25, 2017, 7:00 pmExcelsior, Excelsior Meeting Room
- Parkside Library Book Club
- Tuesday, July 25, 2017, 7:00 pmParkside, Parkside Teen's Area
- Rincon Literario
- Wednesday, July 26, 2017, 6:00 pmMain Library, Paley Room - Floor 3
Upcoming Classes
- Great Books Discussion Group
- Wednesday, July 26, 2017, 6:30 pmWest Portal, West Portal Children's Area
- Great Books Discussion Group
- Tuesday, August 8, 2017, 6:15 pmRichmond, Richmond Meeting Room


text:
The airport hotel provides complimentary high-speed (wired) Internet access and a complimentary airport shuttle. This 2.5-star property also offers business services and conference facilities, laundry facilities and a safe deposit box at the front desk. Further facilities at the air-conditioned, 70-room hotel include a lobby with 24-hour reception, cloakroom, newspaper stand and lift access to the upper floors. A breakfast room, room service, a laundry service and a car park also feature.
The hotel is just minutes from the Westshore business district and local attractions include Westshore Mall, Raymond James Stadium, the International Plaza Mall (3.2 km), and Channelside district, an entertainment/dining multiplex just east of Downtown or the SoHo district. Clients can take the kids to the Florida Aquarium, a glass-domed marine wonderland, or visit the world-famous Busch Gardens (both 19 km away). The city centre is approximately 14 km from the hotel, Clearwater Beach is 28 km away, Ice Palace is some 19 km, Harbour Island is around 11 km away and Ringling Brothers Circus is roughly 96 km away. The hotel is only about 2 km from the Tampa International Airport and Orlando International Airport is approximately 152 km away.
The televisions come with premium cable television channels, HBO and video game consoles. Complimentary high-speed (wired) Internet access is provided along with a direct dial telephone with free local calls. Coffee/tea makers are available in guest rooms. The en suite bathrooms include a shower, bathtub and hairdryer. A double or king-size bed, a radio, hifi, safe, mini fridge, microwave, ironing set and individually regulated air conditioning and heating also feature as standard.
Recreational amenities include an outdoor swimming pool, a sun terrace and sun loungers. There are also on-site fitness facilities.
Guests are offered a complimentary hot and cold buffet breakfast.
The hotel accepts all major credit cards, including American Express, MasterCard and VISA.
From I-275 South take Exit 39 Tampa Airport/Highway 60 West. Go west for 3 km. Turn right at the lights on North Rocky Point Drive and the hotel is on the right.
There are no room charges for children 18 years old and younger who occupy the same room as their parents or guardians, using existing bedding.
Room Facilities:
Direct dial telephone
Satellite cable TV
HiFi stereo system
Internet access
Mini fridge
Tea Coffee
Iron and board
Double bed
King size bed
Individual airconditioned
Individual heating
Wheelchair accessible


text:
Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
does it make a difference if the paralegal sign the attorney's name to the letter?
does it make a difference if the letter id signed with attorney's name with notation that it was singed "by" the paralegal?
Since you tell us nothing about the letter, what it says or how it affects you there is no way to answer you. Lawyers often have staff members send out letters under their supervision.
If you find this answer helpful, please mark it here on AVVO as helpful. In answering you, I am attempting to communicate general legal information and am not representing you (and am not your lawyer). Do feel free to call me at 404-768-3509 if you wish to discuss actual representation (the phone call also does not retain counsel; that requires an office visit and appropriate paperwork). In that a forum such as this provides me with limited details and doesn't allow me to review details and documents, it is possible that answers here, while meant to be helpful, may in some cases not be complete or accurate, and I highly recommend that you retain legal counsel rather than rely on the answers here. (You can also email my office at firstname.lastname@example.com . An email also does not retain my office, but can help you get an appointment set if you prefer not to call). I am happy to discuss possible representation with you. Any information in this communication is for discussion purposes only, and is not offered as legal advice. There is no right to rely on the information contained in this communication and no attorney-client relationship is formed. Nothing in my answer should be considered as tax-advice. To ensure compliance with IRS Circular 230, any U.S. federal tax advice provided in this communication is not intended or written to be used, and it cannot be used by the recipient or any other taxpayer (i) for the purpose of avoiding tax penalties that may be imposed on the recipient or any other taxpayer, or (ii) in promoting, marketing or recommending to another party a partnership or other entity, investment plan, arrangement or other transaction addressed herein. I am also required to advise you, if your question concerns bankruptcy, that the U.S. Congress has designated Ashman Law Office as a debt relief agency that can help people file bankruptcy.Ask a similar question
I agree although it's slopy practice it's acceptable and commonplace in the legal profession unless of course it's a legal agreement in letter form
I agree on the sloppy practice. Personally, nothing leaves my office unless I personally sign, read and review it (no matter who helps write it).
In the past few months, I've received several letters signed by the President, as well as a signed photograph. I don't think I can get much for them, even if he wins. Abraham Lincoln had his secretary (Stoddard) sign most of his routine correspondence, but Stoddard's signature, with a "by" would appear on the document.
If the letter is signed "Joe Blow, Esq. by P.L.", and is simply a cover letter, or routine request for information, such as a request for medical bills in a car crash case, fine. Where my staff has signed letters of this sort in their own name, stating their non-lawyer positions, I have frequently gotten back correspondence addressed to the staff member as a lawyer, with a greeting , "Dear Attorney _______". This could turn out to be unamusing under certain circumstances.
If the letter contains legal substance or advice, it should always be signed by the attorney and nobody else. In ancient times, say pre-1990, when legal secretaries roamed the earth, and men spoke only to dictaphones, and women transcribed them (men being congenitally unable to type, or perhaps to spell), the dictator's and dictatee's (who may have been the actual author, where the dictation was merely an outline) initials, separated by a colon, would appear at the bottom left of the letter.
It would be improper for a paralegal to sign the lawyer's name without the "by",
The foregoing answer does not establish an attorney client relationship, is not confidential, and should not be relied upon in place of an actual consultation with an attorney. Mr. Boone is licensed to practice in Massachusetts, before the U.S. Tax Court, and the Federal District Court of Massachusetts. Most initial consultations are free. Further information is available on my profile and at www.boonehenkofflaw.com.Ask a similar question
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text:
Hey, I've been too busy drinking beer at Mardi Gras and parades to blog about doing so, which is a real shame. I am attending Top of the Hops this weekend and will report back on that...
Also, hats off to the Avenue Pub for keeping craft beer a-flowing through the parade season! Muses Thursday and Mardi Gras day I was so happy to have access to that delicious beer. The Avenue also is now busting into some amazing post-Mardi Gras beers that are delicious.
I also tip my hat to Cork & Barrel, who provided me with several delicious canned beer choices, including but not limited to, Fuller's London Pride! How awesome is that?
I part with a final recommendation: if you have the chance to try NOLA Brewing's latest beer, Flambeau Red, on cask, DO IT. DOOOOOOOOOO ITTTTTTTTTT.
God, that's good stuff. Perhaps the best cask ale I've had this side of the Atlantic.


text:
\nSI's 2008 NFL Scouting Reports\n\t\n\n\t\t\t\t\t\n\n\t\n\t\t\t\n\n\t\t\t\t\t\t\t\t\t\nNew England Patriots\n\nProjected Finish: 1st in AFC East\n\n\t\t\t\n \n\n\n\n\n Mankins, a no-name turned Pro Bowl pick, typifies the Patriot way.\nAl Messerschmidt/Getty Images\n\n\n2008 Schedule\n \n\n\n\n\nSeptember7 KANSAS CITY14 at N.Y. Jets21 MIAMI28 Bye\n\n\nOctober5 at San Francisco12 at San Diego20 DENVER (M)26 St. Louis\n\n\n \n\n\nNovember2 at Indianapolis9 BUFFALO13 N.Y. JETS (T)23 at Miami30 PITTSBURGH\n\n\nDecember7 at Seattle14 at Oakland21 ARIZONA28 at Buffalo \n\n\n\n\n\n\nSPOTLIGHT\n \n\n\nFernando Bryant, Cornerback: The Patriots have had a good run at left corner with Pro Bowl picks Ty Law and Asante Samuel, and now they're looking for this 31-year-old free agent to continue the tradition. If Bryant, who spent five quality years with the Jags before going to the Lions, still has his old coverage skills, they're onto something special.\n\n\n\n\n
It's business as usual: Replacement parts have been found, everyone's locked in, the machine rolls on to the Super Bowl.
\n
The 2002 Super Bowl -- Patriots 20, Rams 17 -- had been over for a few hours,and Bill Belichick, the New England coach, shook his head and mumbled to the fewpeople near him, "Can you believe we won the Super Bowl with this?"
\n
It was hard to believe then that Belichick's collection of castoffs andreclamation projects had become world champions. Now it's not so hard. In an erain which dynasties are supposedly impossible because of the salary cap, freeagency and so forth, the Patriots under Belichick have marched through the 21stcentury like medieval warlords. In the last seven years they've won three SuperBowls in four appearances, and they're overwhelming favorites to be back thisyear.
\n
Oh sure, there's always some tweaking to do. Two years ago they wouldn't paythe price to keep David Givens and Super Bowl MVP Deion Branch, so off wenttheir two top wideouts. "Give me wide receivers," Tom Brady said after the 2006season, in one of his few public shows of annoyance. So in came a supposedlywashed-up Randy Moss, who had an All-Pro year, and Wes Welker, Brady's magicianon the hot reads, who tied for the league lead in receptions.
\n
This year's draft was for defense, for need. Most Patriots drafts are.Thirty-nine-year-old Junior Seau was commended for his faithful service but wasnot invited back. His spot, the coaching staff hopes, will go to top pick JerodMayo, a size- and-speed linebacker from Tennessee who brings a lot of wallop butadmitted in camp that Belichick's defenses, at times, were "a blur." There areplenty of veterans to help him. Two corners, including All-Pro Asante Samuel,were lost to free agency, but two more, Jason Webster and Fernando Bryant, camein by the same route, from Buffalo and Detroit, respectively.
\n
People know better, by now, than to question personnel director Scott Pioli'sdrafts and trades. How many players have left the club and blossomed elsewhere?Almost none. Who, aside from the most obsessed draftniks, had ever heard ofLogan Mankins before New England took him with a first-round pick in 2005? Butin three years he has become one of the NFL's best guards.
\n
The machine grinds on. Distractions are not allowed. You think Spygatecouldn't have been a major distraction last year? Three quarters of a milliondollars in fines from the league office because of the illicit videotaping, lossof a No. 1 draft choice, ugly stares on the street. Some distraction. The seasonended 18-1, and if the Giants' Eli Manning hadn't pulled a Houdini and squeezedout of a rush, and David Tyree hadn't pretended the ball was a long-distancephone call and clasped it to his ear, the Pats would have been 19-0 and SuperBowl champs.
\n
Distractions are kept to a minimum around the Patriots, thanks to a net ofsecurity around the club that's tighter than a nosetackle's jersey. Mysterysurrounds all players on the PUP (Physically Unable to Perform) list. The leaguepolicy on disclosure is muddled, more like, "well, it would be nice if they didcome clean." Not the Patriots. When strong safety Rodney Harrison came off thePUP list last month, he was asked, "Why were you on it?"
\n
His eyes sparkled. "Now you know better than to ask me something like that,"he said.
\n
Brady addressed reporters when he first came to camp and didn't speak againfor another three weeks. Moss didn't meet with the media at all untilmid-August.
\n
Whipped dogs, that's what the Boston press corps covering the Patriots hasbecome. Belichick's daily press conferences are now exercises in how many wayshe can say, "All we're doing is trying to get better." Assistant coaches areoff-limits. The ring of security is tight. And it will be strange indeed if thisteam is not standing front and center in Raymond James Stadium in Tampa comeFebruary.
\n
 -- Paul Zimmerman
\n\n\n\n\t\n\n\t\t\t\t\t\n\n\n\n\n \n


text:
Re: Questions for the Group #2
Expand Messages
- Nancy,
I was hoping to have more members in our Yahoo group than we
currently have before going to the Senate Committee for Health,
Education, Labor, and Pensions.
If you want to contact the Committee, either individually or as a
group, I'd be happy to facilitate that in any way I can.
What happens when you call Senator Clinton's office? Do they refuse
to talk to you?
--- In firstname.lastname@example.com, FourDirect@a... wrote:
> Kevin,
> Since I am getting NO help from Hillary Clinton, how do we go
about reaching
> out to these folks? Sorry for the long delay in responding from
> Nancy
- Kevin,
I just got this listserv posted on the NY RPCV group website today so maybe more will come out of the woodwork. I'd like to have more, too.
Working on photocopying all my stuff just now as I'm going to be in the neighborhood of the USDOL NY office tomorrow and will hand deliver everything for the 9th time.
Your message has been successfully submitted and would be delivered to recipients shortly.


text: Breadcrumb Home / Schoolword What Does It Mean? nefarious --Listen to the pronunciation: WAV format or AU format Dracula has been called nefarious because he 1. always wore black. 2. was especially wicked. 3. was extremely nervous. Yesterday's Word Quiz | Tomorrow's Word Quiz Analogy Quiz Spelling Bee Advanced Word Quiz Infoplease Daily Almanac


text:
Formation and Evolution of Titan's Atmosphere
- Received:
- Accepted:
- Citations
- 136 Downloads
The origin and evolution of Titan's enigmatic atmosphere is reviewed. Starting with the present-day volatile inventory, the question of what was the original composition on Titan and how a satellite of similar size to other Galilean moons managed to acquire and hold on to the required material is discussed. In particular the possible sources and sinks of the main mother molecules (nitrogen, methane and oxygen) are investigated in view of the most recent models and laboratory experiments. The answers expected to be provided by the instruments aboard the Cassini-Huygens mission to some of the most prominent current questions regarding Titan's atmosphere are defined.
KeywordsSatellites Titan atmospheres Solar System infrared space missions
Unable to display preview. Download preview PDF.
Copyright information
© Springer Science + Business Media, Inc. 2005


text: Chicken Wing Face Lock (チキンウィングフェイスロック chikin wingu feisu rokku) is a throw usable by King. Retrieved from "http://tekken.wikia.com/wiki/Chicken_Wing_Face_Lock?oldid=53686" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: King Moves


text:
Officials and mental health experts in Broward County, in the Miami area, are trying to figure out if there's any connection between five suicides by hanging that have taken place in the last three weeks, The Miami Herald reports.
Broward Medical Examiner Joshua Perper tells the paper that he's never seen such a string of suicides in his 20 years of experience.
"I don't remember reading about reports of unrelated individuals committing suicide in a public space or park," Perper says. "It would be a first."
The dead ranged in age from 22 to 62. Two of the bodies were found together.
The oldest, Robert Ford of Jupiter, Fla., suffered from several illnesses, Coral Springs police Lt. Joe McHugh said.
Another, Nikole Baldomero, 24, was upset because she was unemployed and unable to pay her bills, said her mother, Vivian Baldomero, who added, "But nothing you would have seen showed anything majorly wrong."
A 2008 report by the Florida Department of Health showed Broward had the most suicides in Florida, the Herald reports.
Charles Nemeroff, chairman of the Psychiatry Department at the University of Miami, is calling the suicides "a statistical anomaly."
(Posted by Melanie Eversley)
Doug is an unrepentant news junkie who loves breaking news and has been known to watch C-SPAN even on vacation. He has covered a wide range of domestic and international news stories, from prison riots in Oklahoma to the Moscow coup against Mikhail Gorbachev. Doug previously served as foreign editor at USA TODAY. More about Doug
Michael Winter has been a daily contributor to On Deadline since its debut in January 2006. His journalism career began in the prehistoric Ink Era, and he was an early adapter at the dawn of the Digital Age. His varied experience includes editing at the San Jose Mercury News and The Philadelphia Inquirer.
Also: Follow On Deadline on Twitter


text: Hello. I had deleted a user from my forum userbase and then all of a sudden my forums started shooting errors any time I want to see the homepage. Code: Undefined offset: 32 XenForo_Application::handlePhpError() in RCBD/RecentStatus/Model/StatusList.php at line 108 RCBD_RecentStatus_Model_StatusList::getStatusArray() in RCBD/RecentStatus/Controller/Public.php at line 15 RCBD_RecentStatus_Controller_Public->actionIndex() in XenTrCom/TodayBirthday/Controller/Public.php at line 6 XenTrCom_TodayBirthday_Controller_Public->actionIndex() in XenForo/FrontController.php at line 310 XenForo_FrontController->dispatch() in XenForo/FrontController.php at line 132 XenForo_FrontController->run() in /home/auroramn/public_html/forums/index.php at line 13 Any ideas?


text:
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text:
Molecular ecological network analyses
Networks analysis
Understanding the interaction among different species within a community and their responses to environmental changes is a central goal in ecology. However, defining the network structure in a microbial community is very challenging due to their extremely high diversity and as-yet uncultivated status. Although recent advance of metagenomic technologies, such as high throughout sequencing and functional gene arrays, provide revolutionary tools for analyzing microbial community structure, it is still difficult to examine network interactions in a microbial community based on high-throughput metagenomics data.
Here, we describe a novel mathematical and bioinformatics framework to construct ecological association networks named molecular ecological networks (MENs) through Random Matrix Theory (RMT)-based methods. Compared to other network construction methods, this approach is remarkable in that the network is automatically defined and robust to noise, thus providing excellent solutions to several common issues associated with high-throughput metagenomics data. We applied it to determine the network structure of microbial communities subjected to long-term experimental warming based on pyrosequencing data of 16 S rRNA genes. We showed that the constructed MENs under both warming and unwarming conditions exhibited topological features of scale free, small world and modularity, which were consistent with previously described molecular ecological networks. Eigengene analysis indicated that the eigengenes represented the module profiles relatively well. In consistency with many other studies, several major environmental traits including temperature and soil pH were found to be important in determining network interactions in the microbial communities examined. To facilitate its application by the scientific community, all these methods and statistical tools have been integrated into a comprehensive Molecular Ecological Network Analysis Pipeline (MENAP), which is open-accessible now (
The RMT-based molecular ecological network analysis provides powerful tools to elucidate network interactions in microbial communities and their responses to environmental changes, which are fundamentally important for research in microbial ecology and environmental microbiology.
In an ecosystem, different species/populations interact with each other to form complicated networks through various types of interactions such as predation, competition and mutualism. On the basis of ecological interactions, ecological networks can be grouped as antagonistic, competitive and mutualistic networks
Various network approaches have been developed and widely applied in genomic biology
High-throughput technologies such as microarrays and high throughout sequencing have generated massive amounts of data on microbial community diversity and dynamics across various spatial and temporal scales
Overview of MENA
An ecological network is a representation of various biological interactions (e.g., predation, competition, mutualism) in an ecosystem, in which species (nodes) are connected by pairwise interactions (links)
The whole process of MENA can be divided into two phases and each phase is comprised of several major steps (Figure
Figure 1
Overview of the Random Matrix Theory (RMT)-based molecular ecological network analysis
Overview of the Random Matrix Theory (RMT)-based molecular ecological network analysis. Two major parts are included, network construction and network analyses. In each of them, several key steps are outlined.
Figure 2
Process of random matrix theory-based approach for automatically detecting threshold to construct molecular ecological networks
Process of random matrix theory-based approach for automatically detecting threshold to construct molecular ecological networks.
Molecular network under experimental warming
Here we used 16 S rRNA gene-based pyrosequencing data from a long-term experimental warming site
The robustness of MENs to noise
In order to examine the robustness of MEN approach to noise, different levels (1 to 100 % of original standard deviation) of Gaussian noise were added to the warming dataset. Once various levels of noise were added, new correlation matrices based on these noise-added datasets were calculated. The same similarity threshold used for the original datasets was used for defining adjacency matrices in the new datasets. When less than 40 % noise was added, roughly 90 % of the original OTUs were still detected in the perturbed networks (Figure
Figure 3
The robustness to noise of RMT-based MEN construction
The robustness to noise of RMT-based MEN construction. Ineasing levels of Gaussian noise were added to the pyrosequencing datasets under experimental warming. The mean of noise was zero and standard deviation (σnoise) was set to 5, 10, 20, 30 to 100 % of the average of relative abundance of whole dataset. The thresholds (
The overall MENs topology
Scale-free, small-world, modularity and hierarchy are common network properties in many complex systems (Table
Modular structure
Modularity is a very important concept in ecology. It could originate from specificity of interactions (e.g. predation, pollination), habitat heterogeneity, resource partition, ecological niche overlap, natural selection, convergent evolution, and phylogenetic relatedness, and it could be important for system stability and resilience
We used several methods, including short random walks
Figure 4
The submodules of the warming pMEN
The submodules of the warming pMEN. (A) The network graph with submodule structure by the fast greedy modularity optimization method. Each node signifies an OTU, which could correspond to a miobial population. Colors of the nodes indicate different major phyla. A blue edge indicates a positive interaction between two individual nodes, while a red edge indicates a negative interaction. (B) The correlations and heatmap to show module eigengenes of warming pMEN. The upper part is the hierarchical clustering based on the Pearson correlations among module eigengenes and the below heatmap shows the coefficient values (
Eigengene network analysis and the modular topological roles
After modules and submodules are determined, the eigengene analysis is used to reveal higher order organizations in the network structure
Different nodes play distinct topological roles in the network
The correlations between network topologies with environmental traits
The relationships between microbial network topology and environmental characteristics can be examined in both direct and indirect ways. Indirectly, as a first step, the OTU significance (
The correlations between module-based eigengenes and environmental factors can be used to detect the modules' response to environmental changes. In warming pMEN, the coefficients (
Figure 5
The correlations between module eigengenes and environmental traits in the warming pMEN
The correlations between module eigengenes and environmental traits in the warming pMEN. The color of each plot indicates the correlation between corresponding module eigengene and environmental trait. Red color means highly positive correlation and green color means highly negative correlation. The numbers in each plot are the correlation coefficient (
Open-access pipeline
To facilitate the application of MENA in the scientific community, an open-access pipeline for MEN construction and analysis (MENAP) was implemented (
Figure 6
An overview of molecular ecological network analysis pipeline (MENAP)
An overview of molecular ecological network analysis pipeline (MENAP).
The network analysis component is further divided into three major parts:
(a) Network characterization. Various network properties are calculated and evaluated, such as connectivity, betweenness, clustering coefficient, and geodesic distance. The module/submodule detection and modularity analyses is performed using fast greedy modularity optimization
(b) Network visualization. An automatic pipeline is constructed to visualize the constructed network. Moreover, the file format for software Cytoscape 2.6.0
(c) Network comparison. Various randomization methods like the Maslov-Sneppen method
Discussion and conclusions
Most previous studies on the biodiversity of microbial communities have been focused on the number of species and the abundance of species, but not interactions among species. However, species interactions could be more important to ecosystem functioning than species richness and abundance, especially in complex ecosystems
The network approach described is based on the transition of two universal distributions from the random matrix theory. A major advantage of RMT method is that the threshold to construct network is automatically determined. In contrast, most other methods studies use arbitrary thresholds, which are usually based on limited knowledge of biological information
Nevertheless, characterizing ecological network of microbial communities poses major challenges. MENs are constructed by the adjacency matrix originated from the pair-wise correlations of relative OTU abundance across different samples. Therefore, a network interaction between two OTUs or genes describes the co-occurrence of these two OTUs or genes across different samples. The co-occurrence might be caused by species or genes performing similar or complementary functions, or shared environmental conditions that microbial species coexist in
A long-held tenet is that the structure of ecological networks has significant influence on the dynamics
In addition to interactions among microbes within a community, MENs allow for analyses of interactions with their environment through correlations with abiotic environmental measurements, which might provide insights on the conditions that have significant impact on the co-occurring organisms. It is also possible to link groups of organisms with biogeochemical measurements to reveal the functional role of organism in biogeochemical processes. These kinds of data are important for generating hypotheses to help explain natural environments that microbial communities reside, which might lead to forecasting responses of microbial communities when environment changes
In summary, our study provides a mathematical/bioinformatic framework for network construction based on metagenomics data such as sequencing
Data standardization
The network construction begins with a data table with
Defining adjacency matrix
Molecular ecological networks can be built on the basis of the measurements of relative OTU abundance in microbial communities. In MENs, each OTU corresponds to a node. Each network corresponds to an adjacency matrix (or interaction matrix),
To define the adjacency matrix, the similarity of OTU abundance across all samples should be measured first. Such similarity measures the degree of concordance between the abundance profiles of OTUs across different samples. Similar to widely used gene co-expression analyses
Determining the threshold by random matrix theory-based approach
The structure of relevance network strongly depends on the threshold value,
Basic concept of RMT
Initially proposed by Wigner and Dyson in the 1960s for studying the spectrum of complex nuclei
RMT predicts two universal extreme distributions of the nearest neighbor spacing distribution (NNSD) of eigenvalues: Gaussian orthogonal ensemble (GOE) statistics, which corresponds to random properties of complex system, and Poisson distribution, which corresponds to system-specific, nonrandom properties of complex systems
The key concept of RMT is to mainly concern with the
After unfolding the eigenvalues, three statistical quantities can be used to extract information from a sequence of eigenvalues, namely, eignevalue spacing distribution
On the other hand, for the correlated eigenvalues,
We use the
Algorithms of detecting the threshold value
The following major steps are used to define the threshold (
(a) Calculate the Pearson correlation matrix,
(b) Obtain similarity data,
(c) Set an initial threshold value,
(d) Calculate the adjacency matrix,
(e) Calculate eigenvalues
(f) To get unfolded eigenvalues, replace
(g) Calculate the nearest neighbor spacing distribution of eigenvalues,
(h) Using the
(i) If
(j) Find a finer scale threshold value by increasing the threshold with 0.01 within the range of [
(k) If H0 is accepted, i.e., the
Once the final threshold value
Calculation of MEN topological indices and general features
Once MENs are determined, various network topology indices can be calculated based on the adjacency matrix (Table
Scale-free, small world, modularity and hierarchy are most common network characteristics of interest
Module detection
Modularity is a fundamental characteristics of biological networks as well as many engineering systems
Several different algorithms can be used to separate modules, including short random walks, leading eigenvector of the community matrix, simulated annealing approach, and fast greedy modularity optimization
Once the network modularity value (
The algorithm of simulated annealing approach usually produces the best separation of the modules by direct maximization of
The algorithm of fast greedy modularity optimization is to isolate modules via directly optimizing the
Identification of key module members
After all modules are separated, each node can be assigned a role based on its topological properties
The within-module connectivity,
Eigen-gene analysis
One of the grand challenges in dealing with high throughput metagenomics data is the high dimensionality. Various statistical approaches are used to reduce dimensions and extract major features, including principal component analysis (PCA), detrended correspondence analysis (DCA), and singular value decomposition (SVD). SVD is an orthogonal linear transformation of data (e.g., microbial data) from the complexity to the comprehensibility
Suppose there are
where both
Assuming that the singular values are arranged in decreasing order, the first column of
The relative abundance profile of the OTUs within a module is represented by the eigen-gene. In addition, the sum of variance of OTU abundances equals to the sum of the diagonal matrix in SVD. Therefore, the percentage of the variance explained by the eigen-gene is given by
Generally, the module eigen-gene can explain approximately 50 % or more of the variance of the OTU abundances in the module
Module membership
Module eigen-gene provides the best summary of variation in relative abundance of OTUs within a module, but it is a centroid of a module rather than a real OTU. In practice, it is always important to understand how close it is between a given actual OTU and its eigen-gene. The correlation of the eigen-gene in module
Random network construction and network comparison
Since only a single data point is available for each network parameter, we are not able to perform standard statistical analyses to assess statistical significances. Similar to the concept of hypothesis testing, the null model is generated to assess the performance of the alternative model. Thus, the random networks are generated to compare different complex networks using the Maslov-Sneppen procedure
Trait-based gene significance measure
In gene expression network analyses, the gene significance (
Relationships of microbial interaction networks with soil variables
To discern the relationships between molecular ecological networks and soil properties, Mantel tests can be performed
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
YD carried out the analysis, constructed the pipeline and wrote the article. YJ carried out part of method development and method writing. YY provided the RMT method and contributed part of the discussion. ZH contributed the paper writing and oversight the work. FL provided the RMT method. JZ provided oversight of the work and helped finalize the article. All authors read and approved the final manuscript.
This work has been supported, through contract DE-SC0004613 and contract DE-AC02-05CH11231 (as part of ENIGMA, a Scientific Focus Area) and contract DE-SC0004601, by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomics: GTL Foundational Science, the United States Department of Agriculture (Project 2007-35319-18305) through NSF-USDA Microbial Observatories Program, the Oklahoma Bioenergy Center (OBC) of State of Oklahoma, and State Key Joint Laboratory of Environment Simulation and Pollution Control (Grant 11Z03ESPCT) at Tsinghua University.
Ecological networks and their fragility
Food-web structure and network theory: The role of connectance and size
Small world patterns in food webs
Non-random coextinctions in phylogenetically structured mutualistic networks
Molecular eco-systems biology: towards an understanding of community function
Network biology: Understanding the cell's functional organization
Identification of genetic networks from a small number of gene expression patterns under the Boolean network model
Inferring genetic networks and identifying compound mode of action via expression profiling
Reveal, a general reverse engineering algorithm for inference of genetic network architectures
Reverse engineering gene networks using singular value decomposition and robust regression
Pe'er D: Using Bayesian networks to analyze expression data
Quantifying cancer progression with conjunctive Bayesian networks
Discovering functional relationships between RNA expression and chemotherapeutic susceptibility using relevance networks
Variations in DNA elucidate molecular networks that cause disease
Analysis of oncogenic signaling networks in glioblastoma identifies ASPM as a molecular target
Conservation and evolution of gene coexpression networks in human and chimpanzee brains
Raab RM, Stephanopoulos G: Elucidation of gene interaction networks through time-lagged correlation analysis of transiptional data
A general framework for weighted gene co-expression network analysis
Reverse-engineering transiption control networks
Constructing gene co-expression networks and predicting functions of unknown genes by random matrix theory
Application of random matrix theory to biological networks
Snapshot of iron response in Shewanella oneidensis by gene network reconstruction
Committee on Metagenomics: Challenges and Functional Applications
GeoChip: a comprehensive mioarray for investigating biogeochemical, ecological and environmental processes
Tu Q
Phylogenetic molecular ecological network of soil miobial communities in response to elevated CO2
Networks in ecology
Compilation and network analyses of cambrian food webs
The network structure of food webs. In: Ecological Networks: Linking Structure to Dynamics in Food Webs. Edited by M. P, Dunne JA
A global network of coexisting miobes from environmental and whole-genome sequence data
Classes of complex networks defined by role-to-role connectivity profiles
Power and Centrality - a Family of Measures
Collective dynamics of 'small-world' networks
Hierarchical organization of modularity in metabolic networks
Characterization of complex networks: A survey of measurements
Efficient behavior of small-world networks
Classes of small-world networks
Community structure in social and biological networks
Modularity and community structure in networks
Application of random matrix theory to mioarray data for discovering functional gene modules
Hierarchical organization in complex networks
Reproducibility and quantitation of amplicon sequencing-based detection
Metagenomic analysis reveals a marked divergence in the structure of belowground miobial communities at elevated CO2
Pyrosequencing-Based Assessment of Bacterial Community Structure Along Different Management Types in German Forest and Grassland Soils
Elevated CO2 stimulates net accumulations of carbon and nitrogen in land ecosystems: A meta-analysis
The Pervasive Effects of an Antibiotic on the Human Gut Miobiota, as Revealed by Deep 16 S rRNA Sequencing
Biological networks: The tinkerer as an engineer
Hierarchical structure and the prediction of missing links in networks
The modularity of pollination networks
Computing communities in large networks using random walks
Finding community structure in networks using the eigenvectors of matrices
Cartography of complex networks: modules and universal roles
Functional cartography of complex metabolic networks
Functional organization of the transiptome in human brain
Geometric interpretation of gene coexpression network analysis
Eigengene networks for studying the relationships between co-expression modules
Social Network Analysis with sna
The igraph library
WGCNA: an R package for weighted correlation network analysis
Finding community structure in very large networks
Integration of biological networks and gene expression data using Cytoscape
Specificity and stability in topology of protein networks
The nested assembly of plant-animal mutualistic networks
The architecture of mutualistic networks minimizes competition and ineases biodiversity
Plant-animal mutualistic networks: The architecture of biodiversity
Enterotypes of the human gut miobiome
Miobial community structure and its functional implications
Diet Drives Convergence in Gut Miobiome Functions Aoss Mammalian Phylogeny and Within Humans
Universality in complex networks: Random matrix analysis
Universal and nonuniversal properties of oss correlations in financial time series
A human gut miobial gene catalogue established by metagenomic sequencing
Transitive functional annotation by shortest-path analysis of gene expression data
Miobial awakenings
Biological robustness
Stability of ecological communities and the architecture of mutualistic and trophic networks
GeoChip-based analysis of metabolic diversity of miobial communities at the Juan de Fuca Ridge hydrothermal vent
Spatial scaling of functional gene diversity aoss various miobial taxa
Mutual information relevance networks: functional genomic clustering using pairwise entropy measurements
Comprehensive strategies to study neuronal function in transgenic animal models
Gene connectivity, function, and sequence conservation: predictions from modular yeast co-expression networks
Random Matrices in Physics
Random Matrices, 2nd edition
Level fluctuations in quantum systems with multifractal eigenstates
Spectral Properties of the Laplacian and Random Matrix Theories
Random matrix analysis of human EEG data
Scale-free networks: a decade and beyond
Emergence of scaling in random networks
Organization of excitable dynamics in hierarchical biological networks
Vecchi MP: Optimization by simulated annealing
Fast algorithm for detecting community structure in networks
The smallest of all worlds: pollination networks
Singular value decomposition for genome-wide expression data processing and modeling
Detection of Disease Clustering and a Generalized Regression Approach
VEGAN, a package of R functions for community ecology


text:
Cat. No. 1094
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Chemical Name: 4-Methyl-1-piperazinecarboxylic acid 6-(5-chloro-2-pyridinyl)-6,7-dihydr
Biological ActivityA non benzodiazepine BZR agonist, with little difference in affinity for the BZ1 and BZ2 subtypes; however its CNS effects differ from those of other BZR ligands and this may be due to interaction with a different subunit.
Technical DataM.Wt:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Certificate of Analysis / Product Datasheet / Safety Datasheet
References are publications that support the products' biological activity.
Munakata et al (1996) Heterogeneous distribution of benzodiazepine receptors among rat neostriatal neurones. Br.J.Pharmacol. 118 820. PMID: 8762113.
Im et al (1993) Potentiation of γ-aminobutyric acid induced chloride currents by various benzodiazepine site agonists with the α1γ2, β2γ2 and α1β2γ2 subtypes of cloned γ-aminobutyric acid type A receptors. Mol.Pharmacol. 44 866. PMID: 7901754.
Goa and Heel (1986) Zopiclone. A review of its pharmacodynamic and pharmacokinetic properties, and therapeutic efficacy as a hypnotic. Drugs 32 48. PMID: 2874974.
If you know of a relevant reference for Zopiclone please let us know.
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BANGOR | Seasons Grille & Sports Lounge had its first full summer of operation in 2013, and the restaurant saw "minor to moderate" foot traffic from Waterfront Concerts, which is surprising, considering the venue is located just across the street, manager Tom Workman said. Seasons decided to beef up …
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S. Grand Cru Lane, Fredericksburg, TX
Luxurious energy efficient hill top home on 34 acres overlooking the Guadalupe River valley. Meticulous attention to build quality and build out from the bones to the finish out make this the low maintenance home for family life, entertaining, or escaping the nearby communities and wine destinations of Comfort, Fredericksburg, Stonewall, and Boerne. South facing caliche slopes and a separate well/electricity supply/ barns and storage on property below the main home create the ideal start for small vineyard.
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Vitamin B2 (riboflavin), vitamin B2 is solid squamated score cracks in the eyes, laceration photophobia affect plavix side. There is a prodigy real existence and medicinal plants, sparing ascorbic acid, even afterward fervent and siccative. Peradventure consist with inflamed because the excoriate and mucosa of the substances. Vitamin B3 (niacin) also to these dignities establish the denominate "vitamin PP» affect plavix side.
Vitamin P has the function till enhance their useful effects; included in the eyes, tearing, photophobia. Thanks till him, the constitution of choler and blood increases. The sources of tocopherol are liver, wheat, oats, barley, cereal grain soybean, pistachios, amygdalae walnuts and decline mast sunflower affect plavix side seeds, carrots, beets, cereal grain parsley, melon, watermelon, grapefruit, pears, figs, the great deep buckthorn, mulberry, melon watermelon, walnuts. GlavRetsept.Ru found out that which is the knack till pile in the degree of density then the weakness power of choosing obtain round.
With the understanding hypovitaminosis developing pellagra.


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If potatoes are exposed to frost, this can turn the starch to sugars and make them taste sweet . It may be nothing to do with the variety, could be just a faulty batch.
We keep hearing about how kids are out of touch with the rhythm of the seasons, so they need to learn that Spring, not winter, is the time when seeds germinate. But they could be doing what I like to do at this time of year: making a plan of the plot and deciding what to grow, choosing and ordering seeds, perhaps preparing the soil by adding some compost and clearing weeds, etc. Then by March they could begin the seed sowing once the soil begins to warm up and the days lengthen. Onion sets or shallots are easy and can go in quite early (but not yet!)
The children will only get discouraged if they sow seeds at the wrong time (which may not even be possible if the ground is frozen). Peas and beans would probably just rot, and it's really too late for garlic. I wouldn't even move strawberry runners at the moment. Somebody somewhere needs to learn a little about horticulture before attempting to start a garden in January!
I often take photos of my best veg (and also my biggest mistakes). I have recently taken up painting, and did a nice "still life" of some beautiful Hooligan squash I grew. Some people in our local art group paint dogs, or trains, or boats, so I think I may make vegetable subjects my speciality, although I can't see many people wanting to commision a portrait of their potatoes or carrots. But it makes sense to paint something you care about, and I love my veg!
So for me it's about quality and beauty rather than quantity.
TPOs and LIsted Buildings are not things the council will overlook - they will knwo about them (they probably initiated them in the first place) and they have to take them into account. Developers can usually plan the development to work around features such as this and incorporate them into the development, so they wouldnt necessarily prevent the permission being granted.
As someone has pointed out, there is a presumption in favour of development, and the fact that lot of local people don't like it is not in iteslf enough enough to prevent it. People almost always oppose new housing developments close to where they live, but there is an urgent need for more housing, so councils need valid "plannng reasons" if they are to reject an application. Otherwise it will just get taken to appeal and the Government inspector will overrule the Council.
The Council will have a Local Development Plan covering the coming years, and if this land is earmarked for housing on this plan, it's likely to go ahead in some form. I don't think the Parish Council can prevent it - as far as I know their role is just advisory.
It is still worth getting involved, though - in a similar case in our village, local people had some input into the final design and density of a housing development, and to the provision of footpaths, car parking, play areas, etc, as well as to the preservation of natural features and protection for wildlife. You may not be able to prevent it entirely, but you could still make a difference.
I think coffee grounds are on the acidic side, so would be good for mulching acid-loving/ ericaceous plants.
Good idea! My favourite garden shoes are just like wellies that have been cut down, so cutting down old wellies should work well.
The flip side of this is when you see your adult children turning into you....
I have a Kindle Fire HDX and love it. It's great for browsing the web as well as for reading books. I like the backlit screen and adjustable font size, which make it easy to read in poor light (but not great in bright sunlight). I use it a lot and need to charge it every day or two. OH is quite envious, as our desktop computer is so slow, so he's getting a Kindle Fire for Christmas! The desktop PC will still be used for writing and storing documents etc.
In most years, bluebells will be over by late May. Forget-me-nots, too, are getting a bit leggy and messy by this date. Also, I find the Spanish bluebells make me wheeze - I can't stay in a room with them, and I think it likely that others would be similarly affected.
Our daughter got married at the end of May this year and wanted blue flowers or plants. Blue is a difficult colour to find, but we managed it: early in the spring I bought trays of lobelia plug plants (blue and white), which I potted on and cossetted, three plants to a pot. They made very pretty table decorations.
If cut flowers would do, cornflowers are a wonderful colour.
We have one like that in our garden in Devon and it's been fine for about 6 winters now without any protection. You didn't say where you live, but it should be OK in mild areas.


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Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
I hv L2(EAD) with different employer than my spouse(L1B ext.) employer.I hv 8.8 yrs of exp.I m requesting my employer to change my status from L2(EAD) to H1B as my spouse's co. is applying her H1B due to her GC process of L1.My employer is saying that initiating H1B while staying on L2(EAD) is not possible.I hv left 6 months on L2(EAD).Also,my previous H1B had expired in 30th Sept 2010. But I didn't leave usa as I had already filed L2 and application was in process. From the month of Aug 2011 I started working with my current employer on L2-EAD. Now I asked my employer to File H1b since I hv passed more than 365 day after previous h1b expired.I raised query ticket to my employers Immegration team but they said due to possibility of rejection they will not file conversion to L2 EAD to H1B.Please advise to understand why and what should be other option in this case or if any other alternative they can follow. Now April is passed. Still H1b quota remained.
Your inquiry is not very clear. As a starting point in reply, while the time spent in L1 status counts towards the 6-year maximum in L1 or H1B status, the time spent in dependent L2 or H4 status do not count towards the maximum in L1 or H1B. You can start a new 6 year-period in H1B only if you have left the U.S. and stayed out for at least one year.
An attorney-client relationship is not formed by my responses to questions on Avvo. My responses are not intended to be legal advice and must not be relied upon as legal advice.Ask a similar question
A change of status to H-1B requires the employer to file an I-129, and requires that you have valid L-2 status until at least September 30.
You should retain an experienced immigration lawyer to review all the facts and advise you accordingly.
J Charles Ferrari Eng & Nishimura 213.622.2255 The statement above is general in nature and does not constitute legal advice, as not all the facts are known. You should retain an attorney to review all the facts specific to your case in order to receive advise specific to your case. The statement above does not create an attorney/client relationship. Answers on Avvo can only be general ones, as specific answers would require knowledge of all the facts. As such, they may or may not apply to the question.Ask a similar question
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text:
Thornbut was a Narnian Red Dwarf who lived during the Golden Age of Narnia, at the time when Prince Cor returned to Archenland.
The first record of Thornbut's history took place as he marched with the Narnian army to join the Archenlanders in their battle against Rabadash.
Thornbut had strict orders from King Edmund to see that Prince Corin was not involved in the battle, but Corin refused to listen, and they brawled.
By very bad luck, during the brawl Thornbut tripped on a loose stone, came flat down on his nose and sprained his ankle. It was not a severe injury, but it did keep him from joining in the battle.
He had short legs, wore brown pants and a tan shirt, and preferred a brown belt. He had black hair and a gray beard. His weapon is unknown.


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Z's Tap House & Grill
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Z's Tap House & Grill Restaurant Review: Bowling alleys usually offer food as mere fuel, something as forgettable as your handicap. So it's a pleasant surprise that Z's Tap House & Grill wins with an array of well-prepared and flavorful pizzas, burgers, salads, sandwiches and sampler platters to satiate your inner champion. You can bowl, shoot pool, play arcade games or kick back in the restaurant/bar area while watching the 16 HD plasma TVs. There's also a nice selection of imported, domestic and craft beers, and 40 brews on tap.
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HomeLab & Science NewsArticlesBacteria-Free Water and Water Purification Systems
Bacteria-Free Water and Water Purification Systems
Many laboratory water purification systems for microbiology and life science applications are designed to produce bacteria-free water. Producing sterile water is relatively easy using an assortment of technologies such as Distillation, UV sterilization, sub-micron filtration and ultra filtration.
In most all situations, the simplest solution to remove all bacteria from a purified water source is the inclusion of a disposable 0.2 absolute micron filter at the terminal position of the dispensing system. This filter will insure the sterility of the water. When the filter clogs in these on-demand water production systems, it can easily be replaced.
All Labconco WaterPro® RO and WaterPro® PS Polishing Stations can accommodate a 0.2 absolute micron filter installed on the nozzle of the delivery gun or delivery nozzle. The optional Hollow Fiber Final Filter may be used with either the WaterPro RO or PS Station.
Once the sterile water exits the 0.2 micron filter, it becomes the responsibility of the laboratory operator or storage device manufacturer to ensure that water remains sterile. There are few sterile water storage devices designed for the laboratory market. Most sterile water storage devices are system components for large drug manufacturing water for injection operations.
Beyond cost and size, these products are complicated due to the follow operation requirements:
- Inlet and outlet 0.2 micron filters for sterile water
- Air Breather valve protected by a 0.2 micron filter
- UV light reactor for redundant sterilization of the stored water
- Sterilization of the water storage vessel and or water using heat and pressure
- Pump/motor for sterile water delivery
Regulatory Note: Most all laboratory water purification systems designed to produce sterile water are not intended for use in human or animal intravenous injection applications.
Water Purification Systems
These point-of-use pure water systems include reverse osmosis systems and water polishing systems.


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