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GHS Labels - Toxic – 2" x 2" - Roll of 100
Toxic substances are poisonous to any person, plant or animal. It is important for employees in the workplace to be informed about toxic substances so they are able to adequately protect themselves. In order to stay compliant with OSHA's GHS labeling system, it is crucial to label all chemicals and hazardous substances based upon their physical, health, or environmental hazards. These GHS Labels - Toxic – 2" x 2" - Roll of 100 complies with the new GHS labeling system and these labels can be used to identify chemicals which feature toxic properties.
Creative Safety Supply is the leader in visual safety. We strive to provide you with all the necessary products to help improve functionality, efficiency, and safety within the workplace. Use these GHS labels to assist in labeling your chemicals accurately with our OSHA-approved, easy-to-understand GHS pictogram labels.
Stickers measure 2" X 2".
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GHS Labels - Toxic - 2" x 2" - Roll of 100


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JOIN OUR EMAIL LIST be informed of workshops, future DVD releases, special promos etc. We won't share your email and you can opt in or out any time!)
The Portrait Society's
18th Annual Portrait Conference
Reston, Virginia, April 14-17
The 18th Annual Portrait Society's Conference is almost here! It will be held in Reston, Virginia.
See Rob's schedule on the Workshop/Classes page.
The Sculpture dvd is out!
At last, Rob's two-disc dvd describing how to create a bust in terra cotta is now out.
View Excerpts
Richard ORmond speaks on sargent at The Art League of Virginia, November 2015
Rob was priveledged to introduce the great John Singer Sargent historian and author, Richard Ormond. Mr. Ormond also happens to be Sargent's grand nephew and had very special insight about the artist's life. Here is the lecture in its entirety.
portrait of Shady Grove Hospital Doner Unveiled, October 2015
Rob's portrait of Potomac, Maryland pioneer Farid Srour was unveiled at the Congressional Country Club in Potomac, Maryland on Wednesday, October 14th. Rob says he enjoyed Mr. Srour's youthful spirit and painting his expressive face, despite his age of 98. Rob has been commissioned to do several more portraits for the hospital.
Rob's article in The Artists Magazine now out
We just received a few copies of the November 2015 issue of The Artist's Magazine. Rob wrote an article for a column called "Road Test" where he reviews his experience with a linen panel and demonstrates by painting on one of the panels in oil. They show the grisaille and the final. Looks good! (pages 62 -64)
Rob to show/demo at Principle Gallery, Alexandria, VA. May 12-June 2, Demo May 29
Rob will be showing at Principle Gallery, 208 King Street, Alexandria, Virginia, along with artist Teresa Oaxaca from May 12 through June 2. The gallery will not be holding an official opening "party" on the 12th, but instead, on separate evenings, each artist will paint a demo from a live model! Teresa will demo on May 15th and Rob will demo on Friday May 29 at 6 PM. For more information please contact Principle Gallery at 703.739.9326 or firstname.lastname@example.com.
Drawing Show at 11th Street Art
New York
May 15th
If you happen to be in the New York area, Rob also has several drawings (a red chalk and a silverpoint) in the show "Nude, a Figure Drawing Exhibition" at Eleventh Street Arts, part of the Grand Central Atelier at 46-06 11th Street, Queens, NY 11101. The opening reception will be held Friday, May 15 from 6-9pm.
"The Classic Portrait" dvd is now out!
This is a four hour dvd in two parts - grisaille (underpainting) the first day then color added the next. Rob uses pigments and mediums. Info can be found on the dvd page.
See a three minute clip showing some EXCERPTS.
Emmy Nomination for our film editor, Adim Lubkin,
Spring 2013
Congratulations to our dvd director and editor, Adam Lubkin, for receiving an EMMY NOMINATION this past spring for the cinematography in one of his short films. He is so understated that he failed to mention this to us until now! Adam, together with Mike Yamrus (camera and authoring) make up the core of our amazing team. We are really fortunate to have them.
Here's a link to Adam's Emmy nominated clip (He directed the desert footage as well as some of the competition footage). http://www.nfl.com/videos/nfl-films-presents/0ap1000000074707/NFL-Films-Presents-Slacklining-hits-the-Super-Bowl
Rob accepted as an "Art Renewal Center Living Master"
June 2013
Rob has been accepted as an "Art Renewal Center Living Master."
Thank you to our friends who suggested Rob apply. It is a wonderful organization that promotes the continuation and renewal of classical realist art as well as a wonderful resource to see many great historical works of art close up.
http://www.artrenewal.org/ for the main page or http://www.artrenewal.org/pages/sitesearch.php for Rob's portfolio page.
American Art Collector, October 2012
Robert Liberace, Renaissance Painter Incarnate
Page 48-53
Some recent blogs by artist and blogger Matthew Innis...
Rob's demo at May's Nationional Portrait Society Conference
Rob's show at Arcadia Gallery
Don't Miss this...
One Man exhibit
This Saturday Evening
arcadia fine arts
New York, NY
Saturday May 26, 2012
Reception from 4-6pm.
Rob will be doing a live demo during the show, so join us!
View photos of our spring 2012 group trip to Edinburgh
"The Figure Sketch in Oil" dvd is now out!
In a single three and a half hour sitting, Rob uses a full color palette to paint the figure in oil. View excerpts (to music in ths clip). Order on the dvd page.
Rob interviewed for American Artist's
"Drawing, The Complete Course" 2011 issue
American Artist has produced a publication entirely dedicated to drawing. Rob is one of several artists interviewed. He gives drawing tips including highlights, core shadows, lines and blending. Produced by Interweave Press.
Rob interviewed for American Artist's
Spring 2011"Drawing" issue
American Artist's Austin Williams interviewed Rob for this article which focuses on ways to inprove your figure drawing, including trying your had at ecorch (modeling the figure in clay) and other important methods such as identifying shadow areas, varying strokes and expressing personal vision.
Rob is an author!
Rob writes an article for American Artist's "Painting Workshop with the Masters" Spring 2011 issue
Rob wrote an article for American Artist. He covers the history and an extensive "how to" of red chalk drawing. It is 12 pages long and shows a step by step demo plus photos of materials list and a lot of his red chalk drawings.
New Youtube short out Spring 2011
"Painted Sketches"
Check out snipets of several of Rob's painting videos on youtube.
The Artist's Magazine, November, 2010
"A Bevy of Brushes"
by Maureen Bloomfield
What are your favorite brushes? In this informative article in The Artist's Magazine editor Maureen Bloomfield interviews Rob along with several other artists about what brushes they use and why.
Rob Provides "Tip of the Week"
for The Artist's Magazine Blog
October 28, 2010
"I generally use Gamsol to clean my oil brushes after each day, and periodically I'll wash them in soap and water to keep them from getting too hard. Sometimes after a demo I will forget to clean my brushes and I find the paint has hardened on the brush. When this happens I use a little citrus-based paint stripper (fewer fumes) to remove the hardened paint, and then I clean with soap and water. After this treatment the brush will lose a little of its spring but as long as it can leave a mark I'll keep it in the studio." - Robert Liberace
See the full blog.
Rob took part teaching with American Artist's
"Weekend with the Masters" 2010
Laguna Beach, California
September 23-26 2010
The event was conceived by American Artist Magazine as a way to celebrate the renaissance we're experiencing in representational art by "having some of the top traditional artists in the country convene for a long weekend of workshops, demos, lectures, and panel discussions". Rob was asked to participate by teaching two full-day workshops and a half-day demo.
Images from Rob's anatomy drawing class. Photos kindly supplied by Jack Scialabba.
Silver Brush Now Selling Robert Liberace Brush Sets
For years Rob has enjoyed the craftmanship and quality of Silver Brushes so was thrilled when Dee Silver of Silver Brush Limited asked if she could tailor two lines of brushes to his needs. For more information on both the figure painting set and the portrait painting set go to Silver Brush.com.
Artist Daily Online Article, April 16, 2010
Understanding Anatomy Leads to Artistic Accuracy"
by Courtney Jordan
During a figure-drawing demonstration, artist Robert Liberace
showed that an attuned focus on and knowledge of anatomy
can lead to strong depictions of the human body.
Read the article
Rob's dvd Reviewed in premier issue of
"American Painting Video Magazine"
(e-magazine covering current representational artists)
This beautifully crafted film produced by Michael Klein (a wonderful realist painter) features studio visits, demos and interviews and art dvd reviews. Artist featured include Jacob collins, Trais Schlaht, Paul Oxborough, Long Island Academy of Fine Art, Rober Armetta, Kerry Dunn, Carlo Russo and a review by artist Mathew Innis of Rob's "Figure in Motion" dvd. This first issue is two hours in length and can be downloaded as a Quicktime movie for $10. We believe it does a wonderful job of demonstrating the current rennaisance of realist art and is worth seeing. For informaiton go to http://www.americanpaintingvideomagazine.com/
Portrait Painting Atelier: Old Master Techniques and Contemporary Application
Rob just received this beautiful hardcover book from the author, artist and teacher Suzanne Brooker. Just released, it focuses on the foundational principles of art, the history of portrait painting during the resaissance and the recent resurgance in classical painting and how it is being preserved, largely, by the atelier movement of today. It discusses techniques and incudes demonstrations. It is packed with beautiful examples of paintings from old masters and artists of today. She has included six of Rob's portraits. It is 208 pages by Watson-Guptill Publications.
Special event:
John Pence Gallery: The Human Figure
February 26 to April 10, 2010
Rob is participating in a classical figure painting show at the John Pence Gallery in San Francisco. If you're in the area it looks to be an amazing collection from many top figure painters. Unfortunately Rob won't be able to make the opening, but it will definitley be a great show to see. See an article about the show in American Art Collector magazine, February 2010, pages 128-131.
Join Rob in SoHo for the opening reception of an exhibition featuring his small sized figure paintings and drawings. In addition, for the first time at the gallery, Rob will be doing a figure drawing demo during the opening. See an article about the show in American Art Collector magazine, March 2010. The Arcadia Gallery is located (in SoHo) at 51 Greene Street (between Broome and Grande Streets) New York, NY 10013. The show will be up through March 27th. If you can't make it to NY, see the entire show online by going to http://arcadiafinearts.com/exhibitions.html and then click on the image. It will come up as a slideshow. You can either click on the painting or click on the arrows to the left or right of the image.
Rob's demo during the show. See more photos from the show.
Mother Teresa Statue at the National Shrine, Washington D.C.
Rob's original maquette of Mother Teresa, 7x15"
If you find yourself in the vicinity of Washington, D.C., you might want to head to the National Shrine to view the new larger-than-life statue of Mother Teresa located in the hall of Saints. It is based on Rob's original terra cotta design and was sculpted from marble by Italian artisans.
The statue was dedicated in September 2009.
"Paint Alexandria" demo, Art League Benefit, May 16, 2009
Rain didn't dampen this year's "Paint Alexandria". Rob painted a scene from the beautiful Carlyle Gardens, about a block from the Torpedo Factory. Held along (or near) the Old Towne waterfront, this two-day plein air event was filled with on-site demonstrations and instructional sessions from some of the artist-instructor members of the Art League.
Unveiling the Portrait of Dasa at Sarasota's "Southern Atelier"
May 2009
Photos taken by Renee Ray
Sign up to receive occasional emailed newsletters and announcements about Rob's classes, workshops, upcoming shows, etc. (or to view previous newsletters).
INStructioNAL DVD's
(go to dvd page for more information)
Anatomy: The Arm and Hand will be out soon.
Online Video Clips:
excerpts from Grisaille: The Painted Sketch.
excerpt from The Alla Prima Portrait.
excerpt from The Figure in Motion.
excerpt from Anatomy: The Torso.
Clips (to music - these are wonderful clips created by our videographer friend Adam Lubkin)
The "Quick Draw" video
"Painted Sketches" video
"This professional quality, well edited, instructional DVD has everything you'll need to the understand the three color chalk method of drawing: a gifted and articulate instructor, a well organized demonstration, and a section on materials."
~ M. Stephen Doherty, Editor-in-Chief, American Artist Magazine (on the Three Color Chalk DVD)
...Rob's tips on drawing are mentioned in Steve Doherty's new blog
Six Tips on How to Draw Anything Accurately.
...Read a full review of two of Rob's dvds at artdvdreview.com.
Also on Youtube:
Two photo galleries of Rob's painting, drawing and sculpture...
Gallery I
Gallery II

subset: commoncrawl


text: Trance by brendan-obrien5 published on 2012-04-10T14:13:47Z A slow, playful soundtrack featuring a solo clarinet accompanied by vibes and an orchestra. Genre Filmscore/soundtrack Comment by Jim Cullen Aus @brendan-obrien5: From what I can gather, Cubase can export MusicXML and MIDI files, both of which can be imported into my preferred composition programme, Sibelius, which uses traditional notation. If you can export in these formats, I should be able to produce a score (I think). 2012/04/25 08:34:52 +0000 Comment by brendan-obrien5 @zagorath: Unfortunately I don't have a score for this. But I'd love to score it out if you're interested! 2012/04/23 14:14:18 +0000 Comment by Jim Cullen Aus Wow, any chance you've got a score of this? Or perhaps a MIDI or MusicXML file? As a clarinetist, I'd love to take a look at performing this! 2012/04/23 13:53:05 +0000 Comment by Jim Cullen Aus I loved this cadence! 2012/04/23 13:47:26 +0000 Comment by Jim Cullen Aus Did the clarinet do something slightly odd here? 2012/04/23 13:46:39 +0000 Comment by brendan-obrien5 @universalmind: Cheers! 2012/04/12 11:15:43 +0000 Comment by Universal Mind Loving the atmosphere of this song. Your style is an entity of its own. Keep making great music!!! 2012/04/10 14:52:59 +0000


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Reynolds Cut-Rite is a fantastic addition to any crafters toolbox. It is ideal for art and craft projects, such as, die cutting and making mosaics. It is also excellent for protecting surfaces. It can be used when using die cutting to help lift off the dies delicately from the paper, without causing damage or having to fiddle with tiny bits of paper. Simply place the Cut-Rite Paper between the die template and cardstock, and run through a die cutting machine. You will find that the cut paper comes out of the die quickly and easily without the need for picking tools.
Cut-Rite represents the highest quality of waxed paper products produced today. It is manufactured with triple coating of paraffin wax. The production process used is wet waxing. The fibres of the paper stock are saturated with hot liquid when the sheet is formed. The paper is then coated with liquid wax on both sides. The wax is therefore forced into the pores and spread over the outside of the paper.
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BK Shuman Photography Photographer
DRayModel Female Springfield, Ohio, US
Mayhem # 3025529
About MeI enjoy being involved with creative individuals and I thrive in spontaneous photo shoots. The human body is such an amazing and versatile canvas on it's own, all it takes is a touch of inspiration to make amazing art.
Have you worked with DRay? Add Credits for DRay ! >>
Credit NotesKeith Munson
Lorin Edmonds
BK Shuman Photography Photographer
GG Digital Media Photographer
Jayne Willard Model
Len Kaltman Photographer
Rodney ODell Photographer
Kelton Photography Photographer
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text:
Empower Others
Getting things done through others
Ideas & Change
If you don't collaborate you are stealing from others
Building a Brand
Personal Brand--Perception!
Corporate world leaders
Level Five Leaders
Entrepreneurial Leaders
Awake to seize the opportunity
Extreme Personal Humility
How do they look?
Who is a leader?
It's Your Choice!
"Tell me who you hang around with and I'll tell you who you are"
Business Brand
"People forget what you did, but people will never forget how you made them feel"
The Medici Effect
The intersection is innovation
Embracing and listening
Word choice and body language
Tell the truth
Model the behavior you seek
Looks do matter
Social media
Don't be a car sales man!!!
Tall, short, handsome, strong?
Skills of a leader?
What about behavior?
Great listener
Communicates clearly
Be polite and genuine
Gain trust
Benefits of empowering others?
Richard Gonzalez 2013
Stress Control
Final result
Embrace Networking!
Present Remotely
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text:
|Douglas Barr
| Paul W. Cooper
Angela Hunt
|8 dec. 2007
|88 de minute
|film de Crăciun
|Hallmark Channel
| Genie Francis
Ted McGinley
|Statele Unite
|Limba originală:
|Disponibil în română:
|dublat sau subtitrat
|? milioane $
|? milioane $
|Urmat de:
|Învață să iubești (2009)
Biletul (titlu original: The Note) este un film de Crăciun american de televiziune din 2007 regizat de Douglas Barr. Rolurile principale au fost interpretate de actorii Genie Francis şi Ted McGinley. Datorită succesului filmului, a avut o continuare, Învață să iubești (Taking a Chance on Love, 2009).
+Cinemagia: Dupa prabusirea unui avion, un jurnalist local gaseste un bilet despre care crede ca a fost scris de unul dintre pasageri. Femeia porneste in cautarea celui caruia i se adreseaza biletul. Aceasta calatorie este una care se dovedeste mai mult una a regasirii de sine si a revelarii propriului trecut.
+Wikipedia: Newspaper columnist Peyton MacGruder (Genie Francis) finds a note addressed simply to 'T', washed up on shore. It appears to be from the victim of a recent plane crash, and carries a message of hope and forgiveness from a father to his child. MacGruder's readership is down on her column (called "Heart Healer"), and the paper is going to dump it unless she starts to write from the heart. Inspired, MacGruder decides to find the intended recipient of the note, all the while logging her journey through her article. As the mystery unfolds, the note affects each person she contacts significantly.
|Genie Francis
|Peyton Macgruder
|Ted McGinley
|Kingston Danville
|Rick Roberts
|Genelle Williams
|Katie Boland
|Maria Ricossa
- Genie Francis as Peyton MacGruder
- Ted McGinley as Kingston "King" Danville
- Rick Roberts as Truman Harris
- Genelle Williams as Mandi
- Maria Ricossa as Nora
- Katie Boland as Christine
- Jim Codrington as Brother Tim Waverly
- Heather Hanson as Taylor Quist
- Gord Rand as Tanner Walton
Diferențe față de roman Edit
- The book does not take place in the winter time, therefore it is not surrounded by the Christmas holiday.
- The book's events take place in Tampa Bay, Florida. In the movie, the events transpire in Middleborough, North Carolina.
- In the movie, Katie Boland's character is named Christine. In the book, she's named Lila.
To support the premiere of the film, Hallmark Channel launched a website on October 22, 2007 called www.WhatWouldYouWrite.com, where viewers could submit their own personalized notes to family and friends and answer the question 'who would you reach out to if you only had a minute?'[1][2] The website has since been disconnected.
The Note became Hallmark Channel's highest-rated film of 2007[3][4] and third highest all-time rating.[5]
Movie Room Reviews stated:
The Note is truly a heartwarming tale Format:Sic that is fun for the whole family. It offers a story with a deep meaning and hidden storyline. It also demonstrates how someone seemingly up against a wall yet continuing to be pushed, can remain resilient.[6]However, DVD Verdict called it "syrupy", but that fans of other Hallmark Channel-type fare will "find a treasure trove of positivity and middle-aged sexual tension."[7]
Due to the success of the movie for Hallmark Channel, a sequel was made,[8] entitled Taking a Chance on Love, which was written by Douglas Barr. (The Note author Angela Hunt did a novelization of Barr's script.) The original cast returned to reprise their roles.
Another sequel, Notes From the Heart Healer, was broadcast on May 12, 2012.
- ↑ Hallmark Channel Press Release 10/17/2008
- ↑ Hallmark Channel Press Release 9/24/2007
- ↑ Christian Cinema Newsdesk 1/8/2009
- ↑ Futon Critic: Hallmark Channel Press Release 12/2/2008
- ↑ Christian Cinema Product Catalog
- ↑ MovieRoomReviews.com The Note Movie Review
- ↑ DVD Verdict The Note Review
- ↑ Futon Critic: Hallmark Channel Press Release 10/17/2008
Legături externeEdit


text:
Happy New year to all and here's a little lifesaver for those of you having difficulty with the DVD audio for our main technique feature Mix clarity and depth in FM209.
Seems the gremlins have stepped in at the duplicators so if you're having difficulty you can get the full folder of audio examples via this download.
All the audio from the feature is here:


text:
Chronic glucolipotoxic conditions in pancreatic islets impair insulin secretion due to dysregulated calcium dynamics, glucose responsiveness and mitochondrial activity
Cell signaling pathways and networks
In the progression towards diabetes, glucolipotoxicity is one of the main causes of pancreatic beta cell pathology. The aim of this study was to examine the
Exposure of islets to chronic glucolipotoxic conditions decreased glucose stimulated insulin secretion
Our results present an integrated view of the effects of chronic glucolipotoxic conditions on known and novel signaling events,
Type 2 diabetes mellitus (T2DM) is a metabolic disorder in which pancreatic insulin secretion does not meet the demands of insulin sensitivity
GSIS involves both glucose oxidation-coupled ATP production and the anaplerotic/cataplerotic pathway-mediated generation of coupling factors that trigger and amplify insulin secretion, respectively
Another well-known role of glucose is augmenting insulin secretion by promoting phospholipase-C (PLC)-mediated hydrolysis of phosphatidylinositol 4, 5-biphosphate (PIP2) into diacylglycerol (DAG) and inositol triphosphate (IP3)
Further, glucose is known to increase insulin content through insulin gene transcription mediated by PDX1 and MAFa
Chronic hyperglycemia (glucotoxicity) and hyperlipidemia (lipotoxicity) have been known to impair beta cell function
Here, we used specific concentrations of glucose and palmitate to study the effects of
To investigate the effects of chronic glucolipotoxicity on glucose responsiveness and insulin secretion, we generated glucolipotoxic conditions in rat pancreatic islets and the NIT1 beta cell line using 16.7 mM glucose and 500 μM palmitate.
Chronic glucolipotoxicity reduces insulin secretion in rat pancreatic islets
To evaluate the effect of high glucose and fatty acid concentrations on insulin secretion, we incubated rat pancreatic islets as mentzioned above for 72 h (chronic glucolipotoxic conditions that mimic diabetic pathology
Figure 1
Chronic glucolipotoxic conditions
Chronic glucolipotoxic conditions
Additional file 1: Figure S1
Chronic glucolipotoxic conditions increase inflammatory cytokines gene expression and cellular stress in pancreatic β-cells. Rat islets or NIT1 cells were cultured under normal conditions (control) or with 16.7 mM glucose and 500 μM palmitate for 72 h (GL). Post 72 h, RNA was isolated from islets and the mRNA levels of IL1b (A) and Nos2a (B) were measured as described in the materials and methods. Both ROS (C) and nitric oxide (D) levels were estimated in NIT1 cells as described in the Additional file
Click here for file
Additional file 2: Figure S2
Chronic glucolipotoxic conditions increase endoplasmic reticulum stress and apoptosis in pancreatic β-cells. NIT1 cells were cultured under normal conditions (control) or with 16.7 mM glucose and 500 μM palmitate for 72 h (GL). Post 72 h, changes in ER stress markers (A) were analyzed using anti-CHOP, anti-BiP and phospho-specific EIF2a antibodies; β-actin was used as the internal control. Both Caspase-3 activity (B) and cell viability (MTT assay) were measured in NIT1 cells as described in the Additional file
Click here for file
To understand the mechanism by which chronic glucolipotoxic conditions reduce GSIS
Glucose uptake and metabolism is impaired under chronic glucolipotoxic conditions
We examined the effect of chronic glucolipotoxic conditions on glucose metabolism in rat pancreatic islets and NIT1 cells maintaining the same experimental conditions used in Figure
Figure 2
Additional file 3: Figure S3
Glucose metabolism is impaired under glucolipotoxic condition. NIT1 cells were cultured under normal conditions (control) or with 16.7 mM glucose and 500μM palmitate for 72 h (GL). Post 72 h, cells were lysed and cellular NADPH levels were measured (A) and culture medium was used for the estimation of lactate levels (B). Data are expressed as mean±SEM and statistical analysis was performed using the unpaired Student's t-test. (*P<0.05, **P<0.01 and ***P<0.001, n=3-4).
Click here for file
We next ascertained the link between malonyl-CoA formation and insulin secretion under chronic glucolipotoxic conditions. To this end, we treated rat islets cultured in glucolipotoxic conditions with high glucose and found a decrease in insulin secretion, as expected. Interestingly, when ATP citrate lyase (ACLY) was inhibited using radicicol
Chronic glucolipotoxicity impairs fatty acid uptake and metabolism
Since chronic glucolipotoxic conditions impaired GSIS, we next investigated its effect on fatty acid metabolism
Figure 3
Chronic glucolipotoxic conditions
Chronic glucolipotoxic conditions
To ascertain whether fatty acid uptake is impaired under chronic glucolipotoxic conditions, we used a BODIPY dye, a non-metabolized fluorescently labelled fatty acid analog. We observed a three-fold increase in fatty acid uptake under chronic glucolipotoxic conditions (Figure
Mitochondrial number/activity and cytosolic ATP levels are reduced under chronic glucolipotoxic conditions
Since a primary outcome of glucose metabolism is ATP synthesis from mitochondria
Figure 4
Chronic glucolipotoxicity
Chronic glucolipotoxicity
These data present the first line of evidence linking a decrease in cellular ATP to a reduction in mitochondrial number and activity under chronic glucolipotoxic conditions.
An increase in cytoplasmic calcium is required for insulin secretion under chronic glucolipotoxic conditions
Since chronic glucolipotoxicity lowered GSIS and glucose metabolism, we investigated its effects on IP3 and cytosolic calcium, known signaling mediators of insulin secretion
Figure 5
Chronic glucolipotoxicity
Chronic glucolipotoxicity
As further confirmation, we ascertained whether L-type voltage gated calcium channels mobilized calcium under glucolipotoxic conditions by studying insulin secretion in the presence or absence of the L-type channel inhibitor, Nitrendipine, NTD
Additional file 4: Figure S4
Cytoplasmic calcium increase is required for insulin secretion under glucolipotoxic condition. Rat islets were cultured under normal conditions (control) or with 16.7 mM glucose and 500 μM palmitate for 72 h (GL). Post 72 h, islets were treated with 11 mM glucose (HG) alone or with inhibitors (5 μM Nitrendipine and 10 μM 2-APB) for 2 h and secreted insulin was measured as described in the materials and methods. Data are expressed as mean±SEM and statistical analysis was performed using the unpaired Student's t-test. (*P<0.05, **P<0.01 and ***P<0.001, n=4).
Click here for file
Insulin synthesis and intracellular insulin content are reduced under chronic glucolipotoxic conditions
Calcium and cAMP are known to influence insulin gene expression through Pdx1
Additional file 5: Figure S5
Chronic GL conditions reduce insulin synthesis and vesicle transport. Rat islets or NIT1 cells were cultured under normal conditions (control) or with 16.7 mM glucose and 500 μM palmitate for 72 h (GL). Post 72 h, RNA was isolated from both islets and cells. The mRNA levels of PDX1 (A) and Insulin (B) were measured in islets and Rab27a levels (C) in cells were measured as described in the materials and methods. Data are expressed as mean±SEM and statistical analysis was performed using the unpaired Student's t-test. (*P<0.05, **P<0.01 and ***P<0.001, n=4).
Click here for file
Figure 6
Chronic glucolipotoxic conditions
Chronic glucolipotoxic conditions
Insulin granule docking is reduced under chronic glucolipotoxic conditions
In animal models of T2DM, the small GTPase, Rab27a is known to be downregulated leading to decreased insulin granule docking to the plasma membrane, thereby lowering insulin secretion
Here, we found that Rab27a mRNA significantly decreased under chronic glucolipotoxic conditions in both pancreatic islets and NIT1 cells (Figure
Figure 7
Taken together, these data provide the first, integrated
Despite intensive research, information about the mechanism of action and intracellular signaling pathways activated by glucolipotoxicity remains limited. Such an understanding has clinical relevance since the ability of the beta cell to increase insulin secretion in response to fatty acids is thought to be a predisposing factor for T2DM
We used 16.7 mM glucose and 500 μM palmitate in this study after evaluating multiple concentrations for their effect on metabolic stress and cell death (unpublished observations). Under these conditions, we confirmed metabolic stress in pancreatic islets and the NIT1 pancreatic beta cell line as seen by the strong induction of ER stress, oxidative stress and inflammation. As previously reported
Under chronic glucolipotoxic conditions
Based on our findings, we propose a model for the effect of chronic glucolipotoxicity on the pancreatic beta cell (Figure
Figure 8
Model for impact of
Model for impact of
We found it interesting that chronic glucolipotoxic conditions impacted multiple cellular processes including insulin synthesis, content and docking. On the basis of our results, we speculate that chronic glucolipotoxicity impacts insulin content most severely compared to insulin gene transcription, docking and secretion. Future studies will be required to get a more complete understanding of the same.
A key finding in our study is the influence of glucolipotoxicity on mitochondrial number/function. This is in line with the notion that enhanced insulin secretion may require an overall increase in mitochondrial activity/number as opposed to an isolated increase in an aspect of mitochondrial metabolism
Increasing insulin secretion is an intensely pursued therapeutic strategy in T2DM. This study yields
Chronic glucolipotoxic conditions comprising high glucose and fatty acid resulted in various defects in key cellular machineries. Glucose sensing machinery involved in uptake and glucose metabolism for insulin secretion was reduced whereas fat uptake and triglyceride storage was increased. Defects in mitochondrial number and activity along with reduced ATP levels were observed under glucolipotoxic conditions. Similarly, beta cells showed increased ER stress, inflammation and apoptosis together with impaired calcium homeostasis. These defects occurred in conjunction with decreased insulin synthesis, insulin vesicle transport, docking and glucose-dependent insulin secretion. Our data provide a first integrated view of beta cell defects across multiple levels under chronic glucolipotoxic conditions.
RNA isolation, reverse transcription and quantitative real time polymerase chain reaction (qPCR)
Isolation and preparation of rat islets has been described in detail in Additional file
Additional file 6
Supplementary methods.
Click here for file
Insulin secretion and content
Islets were isolated from rats (details in Additional file
Western blotting
NIT1 (ATCC) cells or rat islets were cultured with 5.5 mM glucose (control) with or without 16.7 mM glucose and 500 μM palmitate (GL) for 72 h. After incubation, cells or islets were lysed and total proteins were resolved by SDS-PAGE followed by transfer to nitrocellulose membrane. Protein expression and phosphorylation was measured using
Antibodies against Glut2, CD36 (Abcam), BiP, CHOP, p-eIF2a or β-actin (Cell Signaling Technology) and HRP conjugated secondary antibody (Bio-Rad). The protein specific signals were detected using chemiluminescence substrate (Pierce) and were quantified using Image-J software (NIH).
Measurement of glucose uptake
NIT1 cells were cultured with 5.5 mM glucose (control) with or without 16.7 mM glucose and 500 μM palmitate (GL) for 72 h. Post 72 h, cells were washed and incubated in glucose-free medium at 37°C for 30 min followed by incubation with 50 μM of 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose; Invitrogen) for 15min. After lysis, 2-NBDG uptake was measured at 465 nm excitation/540 nm emission, and normalized to total cellular DNA as measured using bis-benzamide at 360 nm.
Measurement of fatty acid uptake
NIT1 cells were cultured with 5.5 mM glucose (control) with or without 16.7 mM glucose and 500 μM palmitate (GL) for 72 h. Post 72 h, cells were washed and incubated in glucose-free medium at 37°C for 30 min followed by incubation with 1 μM of green-fluorescent BODIPY dyes (Molecular Probes) for 10 min. Cells were washed and incubated with 0.4% trypan blue for 5 min to quench any excess dye. Subsequently, cells were washed, lysed and BODIPY uptake was measured at 485 nm excitation/528 nm emission and normalized to total cellular proteins as measured using the Bradford assay (Bio-Rad). BODIPY uptake was represented as % of uptake under control condition.
Estimation of triglycerides
NIT1 cells were cultured with 5.5 mM glucose (control) with or without 16.7 mM glucose and 500 μM palmitate (GL) for 72 h. After incubation, cells were washed with PBS and lysed. Total cellular protein was estimated using the Bradford assay (Bio-Rad) and triglyceride levels were estimated using an enzymatic assay (DiaSys) as per manufacturer's instructions. TAG levels were normalized to cellular protein levels.
Estimation of mitochondrial DNA copy number
Freshly isolated rat islets were cultured under control or glucolipotoxic conditions for 72 h. Post 72 h treatment, islets were harvested in digestion buffer (Tris-10mM, pH8.0, EDTA-1 mM, NaCl-5 mM, SDS-1% and RNAse-A-10 mg/ml) followed by a phenol-chloroform extraction and ethanol precipitation of total DNA. 5 μg DNA was used for quantitative real time PCR. Mitochondrial cytochrome C oxidase 1 (mtCox1) copy number was measured and normalized to nuclear DNA using hypoxanthine guanine phosphoribosyltransferase (HPRT).
Measurement of islet ATP
Rat islets were cultured as in the insulin secretion assay. Islets were incubated in the KRBH buffer containing 2.5 mM glucose for 1h followed by induction with 11 mM glucose (HG) for 1h. After 1 h of incubation, islets were lysed and ATP levels were estimated as per manufacturer's instructions (ATP determination kit, Invitrogen).
Measurement of succinate dehydrogenase activity
NIT1 cells were cultured in 5.5 mM glucose (control) with or without 16.7 mM glucose and 500 μM palmitate (GL) for 72 h. After incubation, cells were washed and incubated in 100 mM potassium phosphate buffer containing 50 mM sucrose, 10 mM sodium azide, 500 mM sodium succinate and 8 mM INT (Iodonitrotetrazolium chloride; Sigma) for 2 h. Cells without sodium succinate were used as a negative control. After 2 h at 37°C, INT was dissolved in DMSO and estimated at 644 nm. The difference in absorbance with/ without succinate was calculated, normalized to total cellular protein and represented as % control SDH activity.
Estimation of islet IP3
Freshly isolated rat islets were cultured under normal condition (control) or under glucolipotoxic condition (GL) for 72 h. Islets were then washed and incubated in KRBH containing 2.5 mM glucose for 1h followed by treatment with HG for 5 min. IP3 levels were measured in the lysate using an immunoassay kit (Cusabio IP3 estimation kit).
Estimation of calcium mobilization
NIT1 cells were cultured with 5.5 mM glucose (control) with or without 16.7 mM glucose and 500 μM palmitate (glucolipotoxic) for 72 h. After incubation, cells were washed with calcium-free KRBH buffer followed by incubation at 37°C for 1 h in Fluo-3-AM calcium indicator fluorescent dye (Invitrogen). Cells were then induced with either low (2.5 mM) or high (16.7 mM) glucose and the fluorescence was measured at 485 nm. The baseline reading was established by reading fluorescence for 1 minute (from t=0 to t=1 minute) at 6 s intervals. The indicated glucose concentrations were added at t=1 minute. After mixing for 5 s, the final reading was taken for 3 minutes (from t=1 minute to t=4 min) at 6 s intervals.
Exocytosis of docked insulin granules
Islets were cultured as in the insulin secretion assay, washed and incubated in KRBH buffer containing 2.5 mM glucose for 1h. Islets were treated with low glucose alone (2 mM) with/without 30 mM KCl in KRBH for 30 min followed by estimation of secreted insulin in the buffer.
Statistical methods
Data are expressed as mean±SEM and significance was calculated using the unpaired Student's t-test. * indicates p<0.05; ** indicates p<0.01; *** indicates p<0.001 compared to the respective control. Unless mentioned otherwise, n=4 across experiments. Miscrosoft Excel was used for statistical analyses.
Competing interest
The authors do not declare any competing interest.
Authors' contributions
BS, RA, YAN, PPM, SN, PCS, RS, KN, SMK and VMK carried out experiments; VMK, SBP and JMR planned/executed the study and analyzed data. NU, MOA, VMK, SMK, SBP and JMR analyzed data and contributed to writing the paper. All authors read and approved the final manuscript.
We would like to thank all the members of the
These studies were supported by Connexios Life Sciences Pvt Ltd., a Nadathur Holdings company.
Islet beta cell failure in type 2 diabetes
Banting Lecture. From the triumvirate to the ominous octet: a new paradigm for the treatment of type 2 diabetes mellitus
Glucolipotoxicity of the pancreatic beta cell
Secondary beta-cell failure in type 2 diabetes-a convergence of glucotoxicity and lipotoxicity
Saturated fatty acids synergize with elevated glucose to cause pancreatic beta-cell death
Inhibition of insulin gene expression by long-term exposure of pancreatic beta cells to palmitate is dependent on the presence of a stimulatory glucose concentration
Fatty acid-induced beta cell apoptosis: a link between obesity and diabetes
Long-term exposure of rat pancreatic islets to fatty acids inhibits glucose-induced insulin secretion and biosynthesis through a glucose fatty acid cycle
Triggering and amplifying pathways of regulation of insulin secretion by glucose
Glucose-regulated anaplerosis and cataplerosis in pancreatic beta-cells: possible implication of a pyruvate/citrate shuttle in insulin secretion
Acute stimulation with long chain acyl-CoA enhances exocytosis in insulin-secreting cells HIT T-15 and NMRI beta-cells
Malonyl-CoA and long chain acyl-CoA esters as metabolic coupling factors in nutrient-induced insulin secretion
ATP-sensitive K+ channels and insulin secretion: their role in health and disease
Regulation of insulin release by phospholipase C activation in mouse islets: differential effects of glucose and neurohumoral stimulation
Activation of PKC is essential for sustained insulin secretion in response to cholinergic stimulation
Protein kinase C delta plays a non-redundant role in insulin secretion in pancreatic beta cells
Glucose-induced cyclic AMP oscillations regulate pulsatile insulin secretion
Protein kinase C activation and the development of diabetic complications
Cyclical and alternating infusions of glucose and intralipid in rats inhibit insulin gene expression and Pdx-1 binding in islets
Insulin granule biogenesis, trafficking and exocytosis
Mechanisms of biphasic insulin-granule exocytosis - roles of the cytoskeleton, small GTPases and SNARE proteins
Hyperglycaemia as an inducer as well as a consequence of impaired islet cell function and insulin resistance: implications for the management of diabetes
Lipotoxicity in the pathogenesis of obesity-dependent NIDDM. Genetic and clinical implications
Glucolipotoxicity of the pancreatic beta-cell: myth or reality?
A sustained increase in plasma free fatty acids impairs insulin secretion in nondiabetic subjects genetically predisposed to develop type 2 diabetes
Glucolipotoxicity: fuel excess and beta-cell dysfunction
Glucolipotoxicity alters lipid partitioning and causes mitochondrial dysfunction, cholesterol, and ceramide deposition and reactive oxygen species production in INS832/13 ß-cells
Rosiglitazone counteracts palmitate-induced beta-cell dysfunction by suppression of MAP kinase, inducible nitric oxide synthase and caspase 3 activities
Glucose and fatty acids synergize to promote B-cell apoptosis through activation of glycogen synthase kinase 3β independent of JNK activation
Fatty acids decrease IDX-1 expression in rat pancreatic islets and reduce GLUT2, glucokinase, insulin, and somatostatin levels
Pancreatic beta-cell glucokinase: closing the gap between theoretical concepts and experimental realities
Reduction of islet pyruvate carboxylase activity might be related to the development of type 2 diabetes mellitus in Agouti-K mice
Effect of glucolipotoxicity and rosiglitazone upon insulin secretion
A role for ATP-citrate lyase, malic enzyme, and pyruvate/citrate cycling in glucose-induced insulin secretion
The ins and outs of fatty acids on the pancreatic beta cell
Mitochondria-derived glutamate at the interplay between branched-chain amino acid and glucose-induced insulin secretion
Properties and calcium-dependent inactivation of calcium currents in cultured mouse pancreatic B-cells
2APB, 2-aminoethoxydiphenyl borate, a membrane-penetrable modulator of Ins(1,4,5)P3-induced Ca2+ release
Protein Kinase C and Calcium Regulation of Adenylyl Cyclase in Isolated Rat Pancreatic Islets
Adenylyl cyclase 8 is central to glucagon-like peptide 1 signalling and effects of chronically elevated glucose in rat and human pancreatic beta cells
Granuphilin is activated by SREBP-1c and involved in impaired insulin secretion in diabetic mice
Long-term exposure to glucose and lipids inhibits glucose-induced insulin secretion downstream of granule fusion with plasma membrane
Identification of the docked granule pool responsible for the first phase of glucose-stimulated insulin secretion
Effects of palmitate on ER and cytosolic Ca2+ homeostasis in beta-cells
Defective fatty acid uptake modulates insulin responsiveness and metabolic responses to diet in CD36-null mice
Facilitation of fatty acid uptake by CD36 in insulin-producing cells reduces fatty-acid-induced insulin secretion and glucose regulation of fatty acid oxidation
Mitochondrial signals in glucose-stimulated insulin secretion in the beta cell
High-dose clevudine impairs mitochondrial function and glucose-stimulated insulin secretion in INS-1E cells


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Tank pressure manways are available in stainless steel SS304 or SS316 (some are special order). Depending on the size of your tank, your tank manway will be either top or side mounted. Rectangular manways are commonly side mounted in mash tuns to facilitate the removal of grain. Elliptical manways are often found on bright beer tanks and fermenters, and offer both an inward and outward swinging door. Shadowless manways are designed to be mounted flush with the exterior of the tanks, thus creating no interior protrusions, or a lip inside, where unsanitary conditions can build up; they are recommended instead of their oval counterparts. Round manways are simply designed to be an access port for pressurized brewing systems. The maximum manway pressure rating is 60 PSI, but all manways are tested at 70 PSI.
Glacier Tanks will accept returns of website standard stocking items, but only if:
Once your items have been received by Glacier Tanks and verified to be unused, we will refund your purchase price, less shipping costs. Returns will incur a 15% restocking fee. Return shipping costs are your responsibility and shall not be paid by Glacier Tanks.
The following items are non-returnable:
Please be sure you understand and agree to any drawings supplied to you prior to purchase of custom or special order items. Payment of deposits for custom or special order items indicates your approval and acceptance of such custom or special order items.


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Where To Shoot for Android
National Shooting Sports Foundation
- November 11, 2013
( 399)
Search for shooting ranges near you, view video shooting tips and find other resources for shooters. Managed by the National Shooting Sports Foundation (NSSF), Where To Shoot is the web's most comprehensive directory of shooting ranges in the U.S. and Canada. This app brings that directory into the palm of your hand. A great resource for all gun owners, hunters and shooters.
NOTE: This app needs access to a users contacts/address book ONLY for savings range address/contact information to the phone address book.
NOTE ** Verizon uses need to have Google Location Services and Standalone GPS enabled to use "Find Near Me" feature.
NOTE: This app needs access to a users contacts/address book ONLY for savings range address/contact information to the phone address book.
NOTE ** Verizon uses need to have Google Location Services and Standalone GPS enabled to use "Find Near Me" feature.
Awful Apparently there are no gun or archery ranges of any kind in the United States. I searched every state on here and nothing popped up. Tried to get it to work for my area 10+ times and nothing.
Nope, those ranges aren't listed Disappointing that the least expensive outdoor ranges aren't found. Also, it seems only the shotgunner ranges are. When I can waste my time and ammo for sport shooting, I'll reinstall. For now my preparations in the full meaning of the law of the land (think 2nd) are taking my full attention in the unfortunate event a progressive (D or R doesn't matter) wins in 2016, I want to be ready for the inevitable 2nd civil war.
Pretty Rough I think NSSF really needs to go back to the company that made this for them and get them to finish it. There's a filter feature but at least in the S7 I can only see 4 options for the filter as opposed too the actual web site which has actually useful filters.
Useful but leaves GPS on When I'm in a place that's unfamiliar this app is very useful for finding out what shooting areas are available. My big problem is it leaves the GPS on until one force-killed the app. If you forget to kill it your battery life is doomed to be very short.
App gives false information Called three different places in new Hampshire that this app gave information about. All of witch didn't have what the app said they did.
Seems like it could work But all in my area are way out of date. Web links are in a format which is pretty old too. Guessing has not been updated for a few years.
What's New
Version 2.0.3
Fix bug with filter view retraction.
Version 2.0.2
Update for 4.4.
Version 2.0.1
Updated Home screen UI.
Added support for searching by State.
Added support for filtering results by certain criteria.
Version 1.2.0
Updated api for 4.3. Added ability to "jump" from marker of range listing on map of multiple plotted ranges to the ranges detail page.
Rangefinder, Scoring, Discounts, Tee-Times, Weather, On-Demand Ordering, More.


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When I try to import or export a tape from our IBM TS3100 tape library it takes over 20mins per tape. It ranges from 20:30 to 20:40 each time. Before the library could do this in under and minute without a problem but lately it's been taking extremely long. So far Ive installed all the latest update for backup exec as well as updated the firmware on the tape library with no improvement.
Note: all other operations the library does (ie. backups) do not seem affected.
I haven't fully ruled out the issue being with the library but it seems less likely....any ideas?
Backup server is running Windows Server 2008 R2 x64. on an IBM x3500
5 Replies
Apr 11, 2011 at 1:31 UTC
Fidelity Systems is an IT service provider.
Not sure if you have this option; but when you select the import/export command, are you doing this through the library device or the software in backup exec?
Apr 11, 2011 at 1:36 UTC
I'm doing it through BE, I never tried going through the library to see if that would give the same result....I'll test and see.
Apr 11, 2011 at 1:42 UTC
Just tested using library web interface and the tape can be exported within a minute. Anyone have any ideas why BE would be so slow doing the same process?
Apr 11, 2011 at 1:58 UTC
Fidelity Systems is an IT service provider.
It sounds like a possible network bottleneck someplace.
Is this a standalone system or shared Server with other applications?
Can you restart the system to see if you notice any difference? Any updates you can perform on the hardware, I.E - firmware for SCSI, BIOS etc. If you are using a branded system, DELL, HP, IBM makes it fairly simple to update some of these components.
If none of the above help, incorporate a spare switch on its own and attach it to Server by itself without anything else included to determine if you see any improvements. Lastly, you can also try to change the drives in BE to see if you get any better performance; sometimes symantec's native drivers that come with BE work better than Microsofts (if your not using BE's).
Apr 11, 2011 at 2:02 UTC
I have resolved the problem. Turns out someone unchecked "Automatically display new alerts" so when I would go to export the media the "Alert" to approve the export would not popup (I forgot that it would even do this). Instead it would just go into the alerts tab and i'm guessing after 20mins of not being approved or cancelled the export it would get automatically approved and complete. So I told it to display new alerts again and problem solved.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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Added on 10-Aug-2005
Courier-Journal,Louisville, Kentucky Review Of ''Killer Queen A Tribute To Queen''Here's a nice review of ''Killer Queen A Tribute To Queen'' from The Courier-Journal in Louisville, Kentucky USA, that also discusses the Queen+Paul Rodgers 2 USA gigs, the Queen+Paul Rodgers live Sheffield single, and all things Queen this summer.New tribute CD adds to the Summer of Queen ''Killer Queen: A Tribute to Queen'' By Jeffrey Lee email@example.com Courier-Journal''Queen singer Freddie Mercury died nearly 15 years ago, and everyone rightly assumed that the band had died with him -- but somehow this has become the Summer of Queen.''''Queen's reunion tour, with former Bad Company singer Paul Rodgers on vocals, sold out 32 arenas in Europe. A handful of United States dates and a stadium tour of Japan are expected to follow suit.''''A single with the new lineup has just been released, featuring ''Reaching Out,'' ''Tie Your Mother Down'' and ''Fat Bottomed Girls.'' It was recorded live at Sheffield Arena in Sheffield, England.''''And now we have ''Killer Queen: A Tribute to Queen,'' starring Los Lobos, Joss Stone, Flaming Lips, Sum 41, Gavin DeGraw and Be Your Own Pet, the teen punk band from Nashville.''''Highlights include the Flaming Lips' ''Bohemian Rhapsody,'' the skateboard-influenced ''Bicycle Race'' from Be Your Own Pet and Los Lobos' ''Sleepin' on the Sidewalk,'' which sounds as if it were filtered through the Beatles' ''Revolver.''''Regrets? There are a few. DeGraw's limp R&B stylings destroy ''We Are the Champions'' and there's a special place in hell for the person who convinced Stone (''Under Pressure'') that her over-the-top shouting is soulful. Jason Mraz tries to imitate Mercury on ''Good Old Fashioned Loverboy,'' which is as pointless as it is annoying.''Source:http://www.courier-journal.com/apps/pbcs.dll/article?AID=/20050809/SCENE04/508090320/1046
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Hurricane Matthew
At this time, Miami-Dade County is monitoring Hurricane Matthew.
Please visit our Emergency website for the latest information on openings and closings in Miami-Dade County.
Boundary Survey Requirement
A Boundary Survey is required when filing for a public hearing, and also for a building permit or zoning improvement permit if the property is contiguous to a municipal boundary. The boundary survey shall depict the location of any municipal boundary on or across the property being surveyed. The boundary survey submitted shall have been updated within one year from the date of an application filed. The requirement is per County Ordinance passed by the Miami-Dade County Board of County Commissioners on September 12, 2002.Back to Top Page Last Edited: Tue Jul 16, 2013 4:05:59 PM
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Three is the first operator to announce tariffs for the incoming Samsung Galaxy Tab - but break those piggy banks, kids, the purported iPad killer will initially only be available on SIM-only one-month plans, and therefore, at full price.
The UK's smallest major network has announced four tariffs for consumers buying the Android-powered Galaxy Tab. If you want to make voice calls (in your face, iPad), you'll want to go for the SIM10 (£10 a month including 100 any network minutes, 2000 Three-to-Three minutes, 3000 texts and 1GB data) or SIM15 (£15 a month including 300 any network minutes, 2000 Three-to-Three minutes, 3000 texts and 1GB data.
However, if you want to keep your current handset as the main mode of calling and texting, you can also pick up the Tab with a data-only, one-month rolling contract. These go for £7.50 for a gig, or £15 for five. Fret not though - you can still make the odd call and send texts at 10p per text, 10p to call Three mobiles or landlines and 25p to other networks.
The network hasn't ruled out the possibility that in future, the device may be available subsidised on long-term tariffs, but if you're aching to own one like, yesterday, you'll have to pay full price. Online retailer Expansys has posted the device at a cost of £679.99, but Samsung says prices have not been confirmed.


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Income protection for General Practitioners
GPs and Locum GPs
As a GP you are likely to earn several million pounds throughout your career and it is essential to consider what might happen should you be unable to practice for a prolonged period of time.
GP Partner
As a GP partner, your practice agreement will state that you will receive full drawings, (if you were off work due to either Illness, Accident or Sickness) usually for up to a period of 12 months. At that point, it would be normal for your practise agreement to be dissolved and your partners would ask you to leave the practice. Some practices have no formal agreements which can cause major problems in the event of illness.
In the event of short term illness the remaining partners will normally cover the absent GP for a period of time, generally no more than 3 months. If the absent partner has not returned at that point it would fall upon the absentee to cover the cost of employing a locum. (See section on Locum Insurance)
There are no formal NHS sick pay arrangements for GP partnerships so it is vitally important that an Income Protection Policy is put in place to protect you with the correct deferred period.
In most cases if you already have arranged an income protection policy when you were working in hospital medicine it will need to be reviewed as it will probably have been set up to match the NHS sick pay benefits of 6 months full pay and 6 months half pay and may not be relevant to your income as a GP.
Salaried GPs
As a salaried GP your sick pay benefits will usually depend upon the type of contract held by the partnership, PCT or Hospital Trust operating the service contract.
A GMS contract would normally follow the sick pay rules of the PCT and would provide benefits in line with the NHS sick pay. Earlier NHS service may be taken into consideration for sick pay claims however the statutory requirement is to provide benefits as though you were a new employee.
Provisions under the Model salaried GP contract
The Model contract offers improved sick leave benefits, in line with hospital doctors.
Under the Model, a practitioner absence from duty owing to illness, injury or other disability shall be entitled to receive an allowance in accordance with the NHS scale contained in paragraph 225 of the Hospital Conditions of Service. This means that those salaried GPs employed under the Model will be able to receive the following sick leave allowances:
- during the first year of NHS service: one months full pay and (after completing four months service) two months half pay
- during the second year of NHS service: two months full pay and two months half pay
- during the third year of NHS service: four months full pay and four months half pay
- during the fourth and fifth years of NHS service: five months full pay and five months half pay
- after completing five years of NHS service: six months full pay and six months half pay.
Calculating years of service for sick leave under the Model
All previous continuous NHS service (including locum service) is aggregated for the purposes of sick leave. Continuous service means without a break of more than 12 months, although there are specific circumstances in which a break of more than 12 months does not mean a break in qualifying service. NHS service here certainly refers to any work undertaken for a PCO and in an NHS hospital.
PMS and APMS practices do not have to adhere to the model contract. It is absolutely vital to establish the provision in your practice as some only offer a very limited level of sick pay. If you are working in a PMS or APMS practice you should ensure that the deferred period under your income protection matches the sick pay available under the contract.
Locum GPs
As a Locum you are self-employed and therefore have NO entitlement to sick pay. It is therefore vital that you have income protection in place.
Cover can be arranged to start after various deferred periods ranging from 1 day to 104 weeks. The shorter the deferred period (the time before a claim will be paid) the more expensive the contract. Advice should be sought.
Most Locum GPs use personal savings as a buffer to cover very short term loss of income through illness or accident and arrange cover to start after 4 weeks, 8 weeks or 13 weeks.
Irrespective of the type of GP contract you have, a long term illness or loss of earnings can have a serious affect on your financial wellbeing. We use our specialist knowledge of the pay structure and benefits packages for NHS employees to tailor income protection cover to the specific needs of doctors. Our advisers have been providing specialist, independent income protection advice to medical professionals since 1996.
Income Protection also known as Permanent Health Insurance (PHI) provides you with a long term regular tax-free income if you become ill or disabled and unable to carry out your usual occupation following a serious accident or prolonged illness.
Benefits are paid tax-free for as long as you are certified as being unable to carry out your SPECIFIC occupation. This could be until you recover and start work again or up to the age you have chosen from the options available, normally to match your planned retirement or upon death if earlier.
How Does Income Protection Work
Income protection is designed to replace earnings, so insurers take into consideration any income you would continue to receive from work and offer a choice of deferred periods,(the time before they would pay benefit). The maximum you can insure for is approximately 65% of gross income and any other income that you may receive. The benefits under the plan will not replace the millions of pounds career earnings that you would receive if you were working but would provide you with enough to pay your bills and maintain a reasonable standard of living whilst incapacitated.
In the event of a long term illness you need an income protection plan specific to your occupation as a GP and one which knits in with any sick pay benefits you receive from the NHS.
There are a number of companies specialising in offering protection in this area, both the amounts and criteria for cover vary greatly from company to company. It is vital that you take advice to ensure that your income protection is occupation specific, will match your sick pay and will not suddenly increase dramatically in cost should the insurer suffer a number of claims and try to pass on the cost to you.
As a medical practitioner, there is a small risk of accidentally contracting HIV/AIDS. The plans we recommend do not have HIV/Aids exclusion for members of the medical & dental profession if they contract the HIV infection accidentally whilst carrying out their normal duties.
Applying For Cover
We offer the opportunity for you to have a face to face meeting or we can call you at your convenience to discuss your needs and will guide you through the many plans available to ensure that you obtain the correct contract for your needs.
By clicking the button below, you can also apply for a quotation. Please follow the instructions to input your data. Please insert your current gross NHS income including banding payment.


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In the 1989 film Field Of Dreams, Kevin Costner plays a farmer who hears a voice in his head advising him "if you build it, they will come." He does what he is told and clears out some of the crops on his struggling farm to build a baseball field. "They" do indeed come. Ghostly baseball players emerge from the cornstalks to take the field. Costner's long-dead dad comes from the corn to play a game of catch with him. And the final aerial shot shows a line of cars (presumably baseball-loving admission-paying spectators) making a pilgrimage to Costner's baseball field.
"If you build it, they will come" has become a mantra for content marketing. Create valuable content and your prospects will find you, and they'll show up ready to sign purchase orders. But marketers know it is a lot harder than that. It takes a lot of planning & preparation, disciplined execution, and collaboration across marketing & sales teams to make content marketing work and to convert it to business.
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Organisation: Le Partenariat
Benifiting country: Senegal
Water supply and electrification of the health centre
After a meeting with the population, where the public authorities and the health district presented the implementation methodology of the project, the work began in September for two weeks. Today the health centre has functional water and electricity.
Setting up a monitoring committee for the project
The committee is composed of a representative of the health district, a representative of the rural community, the health committee and Le Partnership.
The committee met during the different stages of the project to ensure the inclusion of an institutional project and the involvement of population, thereby ensuring the sustainability of the actions undertaken.


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Essendon West
Household type
This topic has been updated with 2016 Census data for the LGA or other primary area only. What's this?
In Essendon West, 37% of households were made up of couples with children in 2011, compared with 31% in the City of Moonee Valley.
Essendon West's household and family structure is one of the most important demographic indicators. It reveals the area's residential role and function, era of settlement and provides key insights into the level of demand for services and facilities as most are related to age and household types.
To continue building the story, Essendon West's Household Summary should be viewed in conjunction with Households with Children, Households without Children, Household Size, Age Structure and Dwelling Type.
Derived from the Census question:
'What is the person's relationship (to each other person in the household)?'
Source: Australian Bureau of Statistics, Census of Population and Housing (opens a new window) 2006 and 2011. Compiled and presented by .id (opens a new window), the population experts.
(Enumerated data)
Compiled and presented in profile.id by .id, the population experts.
Compiled and presented in profile.id by .id, the population experts.
Dominant groups
Analysis of the household/family types in Essendon West in 2011 compared to the City of Moonee Valley shows that there was a higher proportion of couple families with child(ren) as well as a lower proportion of one-parent families. Overall, 36.8% of total families were couple families with child(ren), and 9.0% were one-parent families, compared with 31.3% and 9.6% respectively for the City of Moonee Valley.
There were a higher proportion of lone person households and a similar proportion of couples without children. Overall, the proportion of lone person households was 26.1% compared to 25.0% in the City of Moonee Valley while the proportion of couples without children was 23.1% compared to 23.3% in the City of Moonee Valley.
Emerging groups
The number of households in Essendon West increased by 4 between 2006 and 2011.
There were no major differences in Essendon West between 2006 and 2011.


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Oleg Kalnichevski <ol...@apache.org>
|Re: feedback needed on JIRA issues
|Wed, 11 Aug 2010 13:08:19 GMT
On Tue, 2010-08-10 at 01:06 +0300, Eugen Paraschiv wrote: > Hi, > Can I please get some feedback on some of the opened JIRA issues, such as: > https://issues.apache.org/jira/browse/DROIDS-89 > https://issues.apache.org/jira/browse/DROIDS-90 > https://issues.apache.org/jira/browse/DROIDS-91 > https://issues.apache.org/jira/browse/DROIDS-92 > I have gained some experience with droids in the past month, so I can start > sending the patches for these and perhaps add others. > Thanks. Eugen I am only peripherally involved in Droids development, but if no one else steps in, I can commit those patches. The proposed changes seem reasonable / simple / non-disruptive enough to be committed without a lengthy review process. Oleg
|View raw message


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We'll spirit the young audience away for a musical adventure inside the orchestra with Britten's The Young Person's Guide to the Orchestra. But we promise to bring them back with smiles on their faces!
Norman Huynh, conductor
Pam Mahon, narrator
Pacific Youth Choir and Dance West
Kids Concerts last approximately one hour.
Kids Series Concert
in this Season Ticket package:
Kids Sunday matinee
All concerts are held at the Arlene Schnitzer Concert Hall.


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Skip to comments.DNA From Democrats: Troy Voter Fraud Case Goes to Lab
Posted on 10/22/2010 6:41:23 AM PDT by rightwingintelligentsia
There are various allegations of possible voter fraud across the country, against both parties, but nowhere does there seem to be a more unusual case than in Troy, New York.
A special prosecutor investigating allegations of voter fraud, Trey Smith, is collecting DNA from the majority of the city council...all Democrats. Five city councilmen, including the council president, as well as four other city and county public officials and political operatives, have been ordered to or have had their saliva swabbed for DNA samples to compare to absentee ballots and absentee ballot applications that were allegedly forged.
The investigation centers on what has been called "a massive voter fraud scheme," that involved absentee ballots for the Working Families Party, in September 2009. It has been alleged that Democrats tried to steal the primary election for city council and county legislature, by forging absentee ballots and ballot applications to ensure that their candidates also won the Working Families Party primary line.
'No comment," is what Democratic Council member Gary Galuski told us, as well as several other public officials who are under investigation.
(Excerpt) Read more at politics.blogs.foxnews.com ...
The suggestion was that they were matching the DNA from saliva on the envelopes to the VOTERS. This, of course, was preposterous.
Matching the saliva of suspect absentee ballots to people you think were involved in a conspiracy to manufacture those ballots - (i.e. the corrupt Dems on the City Council) - that is at least feasible (Once you subpoena the DNA of the suspect City Council members).
Very interesting. Voter fraud needs a real hard look in America these days. It is CANCER to a Republic.
Cool. If they liked the envelopes they are caught. They should be hung if guilty.
This say's it all...the corruption is so bad it is looked at as normal!!!
It has to a little furthered than "liked". If you really love and envelope, you leave a little DNA on it. Just sayin, ya know?
This is the throw away line...voter fraud is almost always a democrat thing usually involving union thugs!!!
Agreed. Vote fraud is a direct attack on the entire body politic. The death penalty is a perfectly appropriate, rational, and moral response to such an offense.
I would, however, like to reinstate the traditions of "tar and feathering" and "rail-riding" for these occasions.
Seriously, after the right to life and property, nothing is more dear to our "unalienable" rights than the vote.
They will change the ballots to self sealing with no liking ..I mean licking needed,
I am not interested in platonic relationship with ballots.
The only things that secure our other rights are the ballot and the jury. In our system, the government is *supposed* to be subservient to the people.
we already know that cheating is hardcoded the Dem's DNA...
I just hope the investigators handling this stuff are using latex gloves for their own protection.
Note to self: Next time use a damp sponge. :-)
And rubber gloves.
The jury and the ballot box are His instruments.
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


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This content has been marked as final. Show 2 replies
1. Re: capacity planning - determine Weblogic memory based on available RAMJay SenSharma MiddlewareMagic Feb 7, 2011 8:31 PM (in response to 792878)Hi,
What should be the Heap size cannot be determined by the RAM size 32GB or 64GB. It depends on the Application...Application Functionality, How many Long living objects it creates , how many JNI calls it makes, How many Jars/Classes it loads, How many users requests your application at peak load, what kind of Caching you are using...etc
Above things are the points which can be used to determine the required Heap Size and the JVM tunning options.
There is no standard calculation or Formula available...The only option to determined the required Tunning parameters and JVM settings is...Load Testing and Performance Testing.
Jay SenSharma
http://middlewaremagic.com/weblogic/?page_id=2261 (Middleware Magic Is Here)
2. Re: capacity planning - determine Weblogic memory based on available RAMPHCharbonneau Feb 17, 2011 2:53 PM (in response to Jay SenSharma MiddlewareMagic)Hi user8688761,
Jay brought some very good points and I could not more agree. Proper capacity planning analysis is key in order to do that type of analysis.
If you are interested in 32-bit vs 64-bit VM, our team recently did a comparaison analysis for one of our environment and you can still review the results and what to expect below but you really need to do your own analysis.
You physical RAM requirement will also depend on the # of managed servers that you are planning to deploy.


text: McCall 4473; ©1941; Men's Nightshirt Contents[show] Links to reviews/blog posts Edit Sources/Vendors Edit Gallery Edit [insert your photos of this pattern made up] Wishlist Edit [insert your username, and make sure your preferences allow for people to contact you via e-mail. Do not list your email address here!] Retrieved from "http://vintagepatterns.wikia.com/wiki/McCall_4473?oldid=749932" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Vintage Sewing Patterns McCall McCall's 1940s Nightshirts Nightgowns Mens 1941 Shirttail Hem Convertible Collar Cardigan Neckline Patch Pockets Topstitching Add category Cancel Save


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So we are 3 days into ouir trip in Denmark. We are currently at a boarding school and we wandered up a stair case where we are and found some computers, hurray. It has been sunny and great, the people are so nice and our road manager -Filip, is really fun and funny. We have been eating some interesting sandwiches but mostly the food is good. We are already tired but its been good. I think we have to go and sound check now, I will write more soon. ok...we are at another boarding school again. This is our second to the last one. To be honest I think I am going to be sad to have to leave. The people have been more then great here. Every where we go they have been so welcoming and are so interested in what we are doing and just being our friends. The weirdest part is that I find that their culture here is almost more simliar to mine then Northern Ireland. I suppose its because all the Enlish media here would be moajority American, as where NI would be majority British for obvious reasons. There are just a lot of things that they would have the same words as us in English, I suppose they know American English as opposed to British. Anyways, ya and the people are really relatable, oh by the way, gorgeous. You know how really beautiful people are the exception in Northern Ireland, or America for that fact...well here its more the rule. They are every where and as Britt and Jess have pointed out to me I like the pretty boys and they are deffinitley pretty here. Its great to feel like I am on a "mission" trip again, ya know? Its been so long since I have experienced a new culture. Its put a new fire in me to know more about the world and the different people. I was telling Pete today that this is a country I could see myself living in because there isnt too much culture shock. Its also reawakened in me my desire to experience more of the world. I am so excited to be able to go to Spain and use the language I at least know a bit. I have really been asking God to show me He is working. The thing is that He has answered, and in usual God fashion He is answering but in different ways then I expected. I asked Him to show us unmistakably that He was working through us. We got a text message last night from a girl whos school we were in and she let us know that our message of forgiveness really challenged her to forgive her classmates who had treated her badly for a long time. Wow! Thats cool. I feel, great! I am tired but its so exciting. Remember the days when you were at camp or a mission trip and there was something so romantic about everything? Yeah thats how I feel. Ok there are no guys sweeping me off my feet but I feel all romantic just the same. Its funny how you get older but you are still always just a girl! Ok well I better go...goodnight
Current mood:accomplished
So I did the final part of the whole process of driving. Its taken a good 4 months to do all this, but I got it taxed today so I am ready to get on the road. Wow, its amazing how much freedom I will have. I catch myself thinking, how will I get there? But then I remember, oh ya, I will DRIVE! One of the hardest things about living over here is your lack on independance. One is because you don't have a job so you don't have an abundance of money and the second is its a foreign country so they do everything different here, imagine that? haha Its hard for us who have come from having "lives" and being able to drive here and there whenever and all that comes with it. I said early in January to some friends that living here has made me feel like a child again in many ways. You are so dependant on other people to tell you how things work, show you where to go, and take you there. But, after God let me experience being dependant for a while, I now have this freedom back! On the road again, just can't wait to get on the road again!-Go on Willie!
Current mood:exhausted
life seems crazy at the moment, yet somehow I find time for this thing, haha. We are moving out of our house in Craigavon on Saturday so I have been packing my huge load of crap(where it all came from I do not know) into boxes and suitcases. The guys(AXIOM) are moving to Waringstown, which is not too far from here, but a smaller house...its still really cool tho, we went and saw it last night. Laura has the big room at the front and I will be sharing with her until I move out. I am moving in with another girl called Laura who lives in Lurgan. I was really sad about that at first, I still am to be honest. Its like moving away from my family sooner then needed. But I will always have a bed at the house so I can always stay there if I want. Its also sad leaving this house. Laura Blakley have made a little ritual these past couple days to wander down to the mall and get our coffee and sit and talk about nothing and everything, I will miss that. So here is my schedual for the next wee while (ya like that huh?) March 3rd-off 4th-Concert in Ballymena 5th-Concert in carrikfergus 6th-off 7th-11th-Mission week in Carrikfergus College 12th-Mannafest(our cd launch-woo hoo!) 13-18th-Ballyclare Secondary Mission week 19-27th-OFF!!! 28th-April 8th-Denmark April 9th-Enniskillen COncert/Mannafest 10th-14th-Off/working on training with Suzi 15-17th-LIT retreat 17-29th-to be seen? 29th-My b-day-woo 24! Ok so it sounds like we have breaks a lot...I always thought that of AXIOM last year, but when you work 18 hour days and do that for 5 or 6 days in a row it tends to take it out of you.Anyways I think Suzi (my boss) and I are working some of that time to plan for next year. hopefully the first week in May I will be going for a week to Spain with Britt...yeah! SUN!!! haha Who knows why I wrote all that out....BUT....if you don't live over here I know there is no concept really of what I do...so maybe that will show you a little.??I hope? Thats all, until I have some profound something else to say:)