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Vizio Pays $2 Million Settlement For Tracking TV Viewers
Vizio, one of the biggest manufacturers of TV in the world is paying the Federal Trade Commission and The New Jersey Attorney General more than $2 million to settle a complaint alleging that the company installed software on smart TVs to track users' viewing habits without their permission.
Under the terms of the settlement, Vizio will now have to get express consent to enable that data collection. According to the complaint, this practice has been going on for nearly 3 years. The software was called "Smart Interactivity" and consumers were not told that screenshots of what they were viewing were being sent to Vizio servers and used to target advertising to those viewers.
Headset Helps Legally Blind See
If you were a fan of Star Trek: The Next Generation, you probably remember the special visor worn by Geordi LaForge than enabled the blind crew member to see. Well, just like communicators and tablets and being able to talk to a computer, and all that other good stuff that came to pass, the headset made by eSight helps the legally blind regain their vision.
A camera captures everything in the user's field of vision and enhances the feed, displaying it on two OLED screens in front of their eyes. The screens can be adjusted to a precise position to take advantage of whatever amount of vision the user has while maximizing side peripheral vision. It can help users read and write, view a board in a classroom, and make walking and taking public transport safer. Users can control the device with a handheld remote.
Microsoft Adds Outlook.com Premium Subscriptions
Microsoft is launching its premium-grade Outlook.com email service. The introductory price is $19.95 (though that will increase to $49.99)
Users will get an ad-free inbox and five inboxes with a custom domain name address. (mail would go to email@example.com instead of email@example.com) This service is targeted at small businesses and families who might each want their own ad-free address.
There's already a $20 per year ad-free version of Outlook.com with 5 inboxes for those who don't require their own custom domains. And, of course, Outlook.com is free if you don't mind tolerating the ads.
~ Cynthia
Tags: eSight 3, in the news, mcrosoft, Outlook.com Premium, settlement, Smart TV, visor helps blind see, vizio


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subset: commoncrawl


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Semtech and LoRa™ Alliance Members to Host Alliance All Members Meeting in San Jose April 4-6
Event to include sessions for non-members including hands-on workshop on low-power wide-area networks, live IoT product demonstrations and exciting hackathon competition
Camarillo, California - March 29, 2016
Semtech Corporation (Nasdaq: SMTC), a leading supplier of analog and mixed-signal semiconductors, today announced it will be participating in the 4th LoRa™ Alliance All Members meeting in Santa Clara April 4-6. The Alliance events will include several activities for non-members, including an open house where participants are expected to get a thorough overview of LoRaWAN™-based low-power wide-area networks (LPWANs), live Internet of Things (IoT) product demonstrations from members of the LoRa Alliance ecosystem (LoRaWAN Open Marketplace), a LoRaWAN 101 hands-on training from Microchip, and the opportunity to compete for prizes in a hackathon-style competition sponsored by ARM, MultiTech and the Things Network. The open event is sponsored by Cisco and takes place on April 6 at the Hyatt Regency in Santa Clara, California. The hackathon event on April 5 is free for participants who sign up for the open house.
LoRaWAN 101, sponsored by Microchip, will cover how LoRaWAN has become a ubiquitous standard for secure, carrier grade LPWANs and how it is helping connect the IoT. Attendees will also get to participate in a hands-on workshop to apply what they learn using LoRa-based platforms from Microchip.
LoRaWAN Open Marketplace is where members from the LoRa Alliance ecosystem will provide live demonstrations of their IoT/M2M solutions, which range from modems, modules and sensors, to base stations, network servers and application servers.
LoRa Hackathon (April 5) hosted by ARM, MultiTech, The Things Network and Semtech, will provide all the tools and training users need to create a long range IoT application. Participants will have a chance to win mDot modules, Conduit gateways, development boards and much more.
"The LoRa Alliance Open House is an excellent opportunity for business leaders and developers to see and experience the complete IoT ecosystem and how it all works together to enable the Internet of Things," said Alberto Guerra, Senior Vice President and Chief Marketing Officer, Semtech. "Through real world use cases, hands-on workshops and live product demonstrations, participants should gain a thorough understanding of how LPWANs are enabling entrepreneurs, small businesses, large enterprises and governments to streamline inefficient processes, reduce costs and offer innovative new services."
The LoRa Alliance is a group of 270+ companies that are dedicated to standardizing the LPWANs being deployed around the world to enable IoT, M2M, smart city and industrial applications. The LoRa Alliance is one of the fastest growing ecosystems for enabling LoRaWAN-based networks and IoT applications with an open and standardized model, making LoRa the IoT technology of choice for large technology leaders such as Cisco, Orange and ARM, as well as start-ups focusing on new business models and solutions for IoT.
LoRa Open House and Hackathon Registration Information
The cost is $99 to register for the Open House on April 6. The hackathon on the day before is free to open house registrants. For more information on the open house and hackathon, please visit open house registration page.
About Semtech
Semtech Corporation is a leading supplier of analog and mixed-signal semiconductors for high-end consumer, computing, communications and industrial equipment. Products are designed to benefit the engineering community as well as the global community. The company is dedicated to reducing the impact it, and its products, have on the environment. Internal green programs seek to reduce waste through material and manufacturing control, use of green technology and designing for resource reduction. Publicly traded since 1967, Semtech is listed on the NASDAQ Global Select Market under the symbol SMTC. For more information, visit www.semtech.com.
Forward-Looking and Cautionary Statements
All statements contained herein that are not statements of historical fact, including statements that use the words "will," "expected to," "should," and "enable" or other similar words or expressions, that describe Semtech Corporation's or its management's future plans, objectives or goals are "forward-looking statements" and are made pursuant to the Safe-Harbor provisions of the Private Securities Litigation Reform Act of 1995, as amended. Such forward-looking statements involve known and unknown risks, uncertainties and other factors that could cause the actual results of Semtech Corporation to be materially different from the historical results and/or from any future results or outcomes expressed or implied by such forward-looking statements. Such factors are further addressed in Semtech Corporation's annual and quarterly reports, and in other documents or reports, filed with the Securities and Exchange Commission (www.sec.gov) including, without limitation, information under the captions "Management's Discussion and Analysis of Financial Condition and Results of Operations" and "Risk Factors". Semtech Corporation assumes no obligation to update any forward-looking statements in order to reflect events or circumstances that may arise after the date of this release, except as required by law.
Semtech, the Semtech logo, LoRa and LoRaWAN are registered trademarks or service marks, or trademarks or service marks, of Semtech Corporation and/or its affiliates. Third-party trademarks or service marks mentioned herein are the property of their respective owners.
Press Contact
Ronda Grech, Public Relations, Telephone: (805) 480-2193


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This content has been marked as final. Show 2 replies
1. Re: Siebel Installation Error: File not found ... ses.eai.thirdparty.enWSiebel-Oracle Feb 27, 2013 2:17 PM (in response to 873268)What is the version you are trying to install? If possible provide the part numbers of the files you have downloaded.
2. Re: Siebel Installation Error: File not found ... ses.eai.thirdparty.enuser9354442 Mar 4, 2013 11:27 AM (in response to 873268)I had experienced this exact error. The image creator does not extract all files from the jar files while creating the installable.
I downloaded WINRAR and manually extracted these files from the enterprise jar file into the components folder of the Siebel Install Image. You will have to do this for the enterprise language pack as well.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
undelete formsAsked by WidenerOSL on September 21, 2015 at 12:21 AM
I presume per the subject, that you are looking for a way to restore your forms. To do that you can use the following guide to see the exact steps: How to Restore Deleted Forms?
Do let us know of course if you happen to have any further questions in regards, or if you meant something else and we would be happy to assist with the same.


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We're in a part of the year where the world is waiting for some big new phones to hit stores (Samsung Galaxy S 4, BlackBerry Q10), while eagerly lapping up the drip-drip-drip of rumours about others (iPhone 5S).
So it makes sense that those three devices feature in our top stories of the week.
Here then, are our five most popular stories, ranked in order of clicks:
1. iPhone 5S might have a 12-megapixel camera
Most popular over the last seven days was our story about the iPhone 5S (which may or may not exist) and the rumours that it will pack a 12-megapixel camera with a lower aperture to improve night and low light shooting.
Read the full story.
2. Samsung Galaxy S 4 hands-on review
We went to New York earlier in the year to be part of Samsung's big Galaxy S 4 launch, and it was worth every minute of jet lag. We had a long play with the new superphone and wrote this hands-on review. We'll have a full review, where we live with the phone as if it was our own, very soon.
3. Vodafone gives BlackBerry Q10 details
Since the BlackBerry Z10 arrived and underwhelmed, we have been looking forward to seeing whether the Q10 will fare any better. Network Vodafone has said that it will have the 4G-ready Q10 from 26 April, with a contract costing a minimum of £37 per month.
4. Updates for the Nokia Lumia 620, 820 and 920
If you have been experiencing issues with your new Windows Phone 8 Nokia's, help is on the way. The Finnish firm has announced that it will be pushing out updates and fixes for the Lumia 620, 820 and 920, which will offer improved automatic display brightness adjustment, a fix for intermittent screen blanking during calls, camera issues and problems with weird reboots.
5. Samsung Galaxy Mega launches
Two more phones from Samsung that push the limits of what size a smartphone can be. These two phones are not as high-spec as the Galaxy S III or Note, but still cram a lot in underneath 5.8 and 6.3-inch screens.
The Mega is due to arrive in the UK in the middle of May.
Read the full story


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UK Board 12th Result 2016 Available Soon at www.uaresults.nic.in. Candidates can check the details regarding Uttarakhand Intermediate Result 2016 from the passage given below.
LATEST UPDATE : UK Board 12th Result has been declare now…………!!!!!!!!!!!!!
Click Here To Check UBSE 12th Result 2016 : (Available Now…………….)
UK Board 12th Result 2016
UK Board 12th Result 2016 will be declare soon by UBSE (Uttarakhand Board of School Education) on its official website at (www.uaresults.nic.in). Candidates can check their UBSE Board Result 2016 from the official website of UBSE or from the direct link given below in the article.The Exam was conducted from 10th March to 1st April 2016. A large number of applicants have given these exams to achieve good marks to do the best for their future because class 12th examination is the starting point of the candidate's life. Candidates are requested not to worry about Uttarakhand Board Result 2016. Students who have prepared well for the examinations they will get good marks for sure. Students are advised to bookmark this page so as to get the latest update about Uttarakhand board 12th result 2016. We will be providing the direct link for accessing the UK 12th result 2016. So, All Those Candidates who have participated in the given examination can get the whole detailed information regarding the result, Check Here.
Uttarakhand 12th Result 2016
UBSE (Uttarakhand Board of school education) has a straight structure covering basic education, state council of education, training, research, and secondary education. Every year, a large number of applicants appears in these SSC and HSC exam. Uttarakhand Board organizes SSC exam in the month of March. The Uttarakhand Board official languages are English and Hindi. The board provides study material and syllabus copy to all the candidates studying in this Board. Great news for all students who had appeared for Uttarakhand board 12th results 2016 examinations. Latest news from the Uttarakhand Board of School Education officials that the UK 12th Board results, will be released on 25th May 2016 on official website at (www.uaresults.nic.in). The question paper is published according to the prescribed syllabus copy. Further Details regarding the result, from below.
UK Board 10th Result 2016 Details
- Result Status : Available on 25th May, 2016
- Examination Conducted By : UBSE (Uttarakhand Board of school education)
- Name Of The Examination : 12th board examination
- Date Of The Examination : 10th March to 1st April 2016
- Official Website : www.uaresults.nic.in
- Result Date : 25th May, 2016 (Expected)
UBSE 12th Previous Years Results 2016 :
|Passing %
|92.56 (expected)
Uttarakhand Class 12th Result 2016
A huge number of students has given the 10th class examination from the UK board and now they are waiting to check their examination result. In 2016 UBSE 10th result is releasing on 25th May 2016. A large number of candidates appeared for the Uttarakhand 10th board examination. Total of 1,73,226 students appeared in the Uttarakhand board examinations. Students are advised to keep a regular check so as to get latest update on Uttarakhand board examinations. So, if you are one of them then we are notifying you that the UK board will be declared UBSE 12th class result 2016 on the official website in June 2016. For the reason that, Board every year announced the 12th board result on the official website in May / June. Students need to fill the application form and submit it to your respective principle office. For more information about applying for supplementary exam students can ask their principle for the full format. Candidates can Check the details are provided below.
Uttarakhand Board 12th Result 2016
Students who are waiting deliberately for the results were to be informed that the results will be declared soon by UK Board at (www.uaresults.nic.in). Students can check the result from the direct link provided below in the article. As we all know that class 12th is base of a candidate's career and the marks of class 12th plays an important role in higher education courses. So that's why every applicant wants to get higher marks in the 10th board exams. So candidates who have given this UK Board class 12th exams tried their best to score as high marks as they can. And now they all are eager to know their results and there is good news for them as the board will be going to release the result on 25th May 2016 on the board's official website. Applicants are suggested to follow the some simple steps below to get their results. So all the applicants are now eagerly waiting to get their Uttarakhand Board HSC Results 2016. So here we are informing the applicants that result will be updated soon on the official website given below of the Uttarakhand Board.
UBSE Board 10th Exam Result 2016 Download
Students find any mistake in marks card, then they must contact with the Principal of the respective schools and ask for steps to solve the issue. For the latest updates regarding the 12th result UBSE 2016, you can stay updated with our site where all the news regarding result publication will be constantly updated for everyone concerned. UBSE Board has completed process of making 12th Class March-April 2016 Examination Results. The Candidates who had applied appeared in UBSE Board 12th Class Examination 2016 can check out the UBSE 12th Exam Results by Clicking Bellow allocated direct link, More details about the UBSE Board 10th Results 2016, you can tuned visit here. After the result is published, students can apply for rechecking their mark-sheet. The board will provide opportunity to fill in application in online process to re-evaluate paper. Students will have to apply within 21 days after result publication. Follow The Given Steps to download the result as follow :
- Applicants need to visit the official website – www.uaresults.nic.in.
- Then go to Notice section, click on it.
- Search the UBSE 12th Result 2016 link, click on it.
- Enter all the necessary details and then click on submit button.
- Your Scorecard will appear on your screen.
- Now save it for future use.
All Candidates are advised to keep regular check the on the official website of UBSE at (www.uaresults.nic.in) for more information regarding UBSE 12th Result 2016 or stay connected with the same page at (4result.in) for the latest updates. For any type of queries, or feedback regarding the article you can drop your comments in the comment box given below….


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Saint Clair Family Estate produce a wide range of varieties, including Sauvignon Blanc, Pinot Gris, Viognier, Chardonnay, Riesling, Gewürztraminer, Pinot Noir, Merlot and Syrah, available in four ranges of wines – the Reserve Range, the Pioneer Blocks, the Premium Range and Vicar's Choice.
Pioneer Block 3 43 Degrees Sauvignon Blanc 2015
Three gold medals
BUY NOW $25.90 per bottle, $310.80 per case
12 June 2017
Decanter Win for Saint Clair Family Estate
Saint Clair Family Estate is celebrating an outstanding achievement at the Decanter World Wine Awards 2017. The Saint Clair Pioneer Block 9 Big John Riesling 2016 achieved a Platinum Best in Category award, for the best New Zealand Riesling.
Read more here ....
Trophy Best New Zealand Wine
Saint Clair Marlborough Premium Gruner Veltliner 2015
Australian Alternative Varieties Wine Show 2017
View Major Awards here ....


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ABC News Anchor Elizabeth Vargas Checks Into Alcohol Treatment Program
ABC News Anchor Elizabeth Vargas Checks Into Alcohol Treatment Program
The popular 20/20 news anchor Elizabeth Vargas has checked herself into an alcohol rehabilitation center. As reported by nydailynews.com, someone close to Vargas states that she is in rehab and getting treatment for her alcohol addiction. "She's been there for a few weeks and will continue to be there for another few weeks." the source stated.
Vargas confirmed herself that she is in fact in treatment and will be returning to her position as soon as she is feeling better and ready to carry on her life again.
She elaborated to nydailynews.com: "Like so many people, I am dealing with addiction. I realized I was becoming increasingly dependent on alcohol. And feel fortunate to have recognized it for the problem it was becoming. I am in treatment and am so thankful for the love and support of my family, friends and colleagues at ABC News. Like so many others, I will deal with this challenge one day at a time. If coming forward today gives one other person the courage to seek help, I'm grateful."
ABC said in a statement that they are "proud of the steps" that Vargas has taken. They are grateful for the distinguished work she has accomplished during her time with the network; she is a member of their family and they will support her in any way that they can.
What Is Alcoholism?
Alcoholism is basically defined by an obsession to drink at any time during the day, despite the consequences or circumstances. It has been recognized by the American Medical Association (AMA) as a genetic disease.
Alcoholics drink no matter what, and such drinking is usually followed by extreme behavior, making the person "not themselves". Constant repercussions follow the alcoholic because of his drinking.
The obsession to drink can start at any age for the alcoholic, provided that they are in fact able to reach a supply of alcohol to activate the bodily disease that causes the alcoholic to drink obsessively. Alcoholics have been described as having an "allergy" to alcohol.
This means that, when consumed, their body reacts abnormally to the substance. As a result, the alcoholic needs more and more alcohol to appease the craving, which continues to climb and climb until the drinker can no longer sustain the amount they are drinking and pass out.
Alcohol Poisoning
As a result of this heavy drinking, it is no surprise that alcohol poisoning is a common affliction in the world of chronic drinkers. Over time, their bodies are able to withstand the onslaught of constant drinking, at least for a certain amount of time, a tolerance builds, and the alcoholic is able to appear to carry on a normal life, at least outwardly.
However, such a lifestyle catches up with them, and a large array of serious health problems can come about. The human body cannot take all that alcohol on a daily basis. The liver starts to go, resulting in liver damage and, eventually, a condition known as multiple sclerosis. Other conditions such as heart and liver failure occur, and another bizarre and horrifying scenario knows as "wet-brain".
Wet Brain
As outlined by Hamsnetwork.org, wet brain (known by a few other names, including Wernicke-Korsakoff syndrome) is basically a condition where the brain is unable to absorb the vitamin known as thiamine, or vitamin B1. Alcohol interferes with this process, and with enough alcohol consumed over a long enough amount of time, the brain simply loses its ability to absorb B1.
This occurs when a thiamine deficiency is already occurring, usually from heavy drinking, and the constant bursts of glucose, which is found in most alcohol, keep hitting the brain and start to degrade the white matter. People who have wet brain often display symptoms of bad muscle coordination, confabulation (remembering events that never happened), inability to form new memories, loss of memory to a severe degree and troubled vision, such as constant blurriness and double-vision.
All this, of course, is not to say that Elizabeth Vargas has any of these symptoms or conditions going on with her at the moment. The one and only cure is alcohol treatment and total abstinence. Only then will the onslaught of alcohol to the body cease and the damage will begin to be repaired. The fact that Elizabeth Vargas has gotten into treatment and, hopefully, recovery is a great victory and it is a great possibility we will see a new and healthy woman step out the other side in the coming weeks.


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Daily Commentary – Monday, October 20, 2014 – Officer Darren Wilson Claims He Feared For His Life
- He claims that Michael Brown pinned him in is vehicle as they were struggling for Wilson's gun
Daily Commentary – Monday, October 20, 2014 Download
Posted October 20, 2014 by Klaasend
Assault, Crime, Dana Pretzer, Ferguson, Manslaughter, Racism, Scared Monkeys Radio | no comments
Daily Commentary – Friday, October 3, 2014 – Pipe Bombs Found in Search for PA Shooting Suspect
- Two pipe bombs were found in the woods where 31yr old Eric Frein is believed to be hiding
Daily Commentary – Friday, October 3, 2014 Download
Posted October 3, 2014 by Klaasend
Assault, Crime, Dana Pretzer, Homicide, Manslaughter, Scared Monkeys Radio | no comments
Judge Thokozile Masipa Rules Blade Runner Oscar Pistorius Not Guilty of Premeditated Murder in the Death of Girlfriend Reeva Steenkamp … Still Faces "Culpable Homicide"(Update: Pistorius Found Guilty of Culpable Homicide)
Oscar Pistorius Found Not Guilty of Murder in Death of Reeva Steenkamp … Say hello to South Africa's O.J. Simpson.
This morning Judge Thokozile Masipa ruled the Blade Runner, Oscar Pistorius, was not guilty of premeditated murder in the shooting death of his girl friend Reeva Steenkamp. The shooting took place on Valentine's Day 2013. The judge ruled that the prosecution's evidence to support a charge of premeditated murder was "purely circumstantial." and "the state clearly has not proved beyond reasonable doubt that the accused is guilty of premeditated murder." The judge went on to say that "there are not enough facts." However, Pistorius is hardly out of trouble as he only has been found "not guilty" of the most serious murder charge. He still faces the lesser charges of "culpable homicide" in the shooting death of his girlfriend.
The judge presiding over the trial of Oscar Pistorius, the Paralympic athlete, cleared him of the two most serious murder charges against him on Thursday, almost certainly sparing him a long prison sentence. But then, after appearing to be on the verge of declaring Mr. Pistorius guilty of a lesser crime, culpable homicide, in the shooting death of his girlfriend, the judge abruptly suspended court proceedings for the rest of the day.
"We'll have to stop here and resume tomorrow morning," the judge, Thokozile Matilda Masipa, said less than half an hour after the session had resumed after a lunch break. She did not explain the adjournment.
Judge Masipa then turned to the lesser charge of culpable homicide, which is comparable to involuntary manslaughter, and said Mr. Pistorius had failed three tests to be exonerated of the charge. One considers what a "reasonable" person would have done under the same circumstances.
Reuters – Africa: Pistorius cleared of murder, culpable homicide verdict to come.
"I am of the view that the accused acted too hastily and used excessive force. It is clear that his conduct was negligent," she told the packed courtroom before adjourning until Friday. She also said he had not acted "reasonably".
Earlier, Masipa ruled that prosecutors, led by the combative Gerrie Nel, had failed to prove the 27-year-old intended to kill Steenkamp after an argument.
The defence said Pistorius shot Steenkamp as a result of a tragic accident after mistaking her for an intruder hiding behind a locked toilet door.
As Masipa delivered her not-guilty decision on the primary charge of premeditated murder, Pistorius, who would have faced at least 25 years behind bars if convicted, sat sobbing in the dock, tears streaming down his cheeks.
Culpable homicide – the South African equivalent of Britain's manslaughter – carries up to 15 years in prison but has no minimum sentence.
Although Masipa described Pistorius as a "very poor" and "evasive" witness, she said it did not mean he was necessarily guilty in a case heavily reliant on circumstantial evidence
ABC News: Judge Masipa called Pistorius a "very poor witness" during the murder trial and found he was at times vague and avoided answering some of the prosecution's questions. Despite that, she says this does not necessarily indicate his guilt. From the wording of the judge's decision in the not guilty verdict of murder and premeditated murder,m it seems almost a certainty that she will find him guilty of culpable homicide.
A verdict on the charge of culpable homicide, similar to manslaughter in the United States, still remains. Pistorius also faces two counts of discharging a gun in a public area, as well as illegal possession of ammunition.
If he is found guilty of murder without premeditation, or culpable homicide, he could receive a 15-year sentence as a first time offender. Mitigating factors, such as his emotional state, anxiety levels and disability could result in a lesser sentence.
The Guardian: LIVE updated coverage of the trial of Oscar Pistorius.
UPDATE I: Oscar Pistorius found guilty of culpable homicide
Olympic and Paralympic track star Oscar Pistorius was convicted of culpable homicide on Friday, escaping the more serious charge of murder for the killing of his girlfriend, and will now battle to avoid going to prison.
The 27-year-old double amputee, who became one of the biggest names in world athletics, stood impassively in the dock, his hands folded in front of him, as Judge Thokozila Masipa delivered her verdict.
Pistorius was also convicted of firing a pistol under the table of a packed Johannesburg restaurant but cleared of two other firearms charges – illegal possession of ammunition and firing a pistol out of the sun-roof of a car.
Posted September 11, 2014 by Scared Monkeys
Crime, Legal - Court Room - Trial, Manslaughter, Murder, Not Guilty, Oscar Pistorius - Blade Runner, Sports, You Tube - VIDEO | one comment
Obama Administration Sanctions Release of Illegal Immigrant Murderers, Drunk Drivers & Sex Crime Felons
As reported at The Washington Times, in 2013 the U.S. Immigration and Customs Enforcement released 36,000 immigrants back into the US population that consisted of 116 with convictions for homicide, 43 for negligent manslaughter, 14 for voluntary manslaughter. This is unreal. Hey Jeb Bush, here are more of those folks that came to America illegally out of an 'act of love'. These don't look like misdemeanors Jeb. A note to Republicans, if you think you are going to pass "amnesty" or any immigration bill with Obama in office and a Democrat controlled Senate, you will lose in November and bring about finally the advent of a true Third Party. As Rep. Lamar Smith (TX-R) stated, "This would be considered the worst prison break in American history, except it was sanctioned by the president and perpetrated by our own immigration officials."
Immigration officials knowingly released dozens of murderers and thousands of drunken drivers back into the U.S. in 2013, according to Obama administration statistics that could undercut the president's argument that he is trying to focus on the most serious criminals in his immigration enforcement.
Among the 36,000 immigrants whom U.S. Immigration and Customs Enforcement released from custody last year there were 116 with convictions for homicide, 43 for negligent manslaughter, 14 for voluntary manslaughter and one with a conviction classified by ICE as "homicide-willful kill-public official-gun."
The immigrants were in deportation proceedings, meaning ICE was trying to remove them from the country and could have held them in detention but released them anyway, according to the Center for Immigration Studies, which published the numbers Monday. The Washington Times also obtained the data.
"This would be considered the worst prison break in American history, except it was sanctioned by the president and perpetrated by our own immigration officials," said Rep. Lamar Smith, Texas Republican. "The administration's actions are outrageous. They willfully and knowingly put the interests of criminal immigrants before the safety and security of the American people."
ICE Document Details 36,000 Criminal Alien Releases in 2013
- 193 homicide convictions (including one willful killing of a public official with gun)
- 426 sexual assault convictions
- 303 kidnapping convictions
- 1,075 aggravated assault convictions
- 1,160 stolen vehicle convictions
- 9,187 dangerous drug convictions
- 16,070 drunk or drugged driving convictions
- 303 flight escape convictions
Democrats Courting the Felony Vote … Atty Gen. Eric Holder Urges States to Lift Bans on Felons' Voting
Obama administration looks to get the "felon" vote …
Hmm, do you think those that would abduct and abuse a child the right to vote? Do you think child rapists should have the right to vote? Do you think that child murderers should have the right to vote? Do you think individuals who would partake in the human and sex trafficking of minors and adults should have the right to vote? Do you think an individual that would physically assault a woman, rape and molest a woman and murder a woman the right to vote? Barack Obama's AG Eric Holder does. So who really has a war on woman and children?
Why would anyone want a man like convicted child rapist Eric Bradley or anyone like him who had the ability to be paroled, a commuted sentence or serving time the right yo vote ever!!!
Convicted Child rapist Eric Bradly
Imagine this, Attorney General Eric Holder is urging states to lift bans on laws that make it illegal for felons to vote. Of course this has nothing to do with the studies that show that felons who have been denied the right to vote were far more likely to have voted for Democrats than for Republicans. Of course Holder has no authority to change such law as this is a State's right to pass their own voting laws, but far be it from the Obama administration to over-extend their Constitutional authority. This request by Holder is most likely a non-starter and going nowhere, but that will not stop Holder and the Obama administration to use another issue to be divisive along racial lines. This is just more gutter politics from the Obama administration looking to scrape up any vote they can from the bottom of the barrel.
Really? We do not have voter ID laws in the United States to make sure that there is not voter fraud going on in elections, but instead Eric Holder wants to allow felons to vote after they have served their sentence. Truth be told, he probably would not care if they had the right to vote while they were serving time in prison. Its an Obama world.
Hmm, Holder wants felons to vote but thought the above NBPP thugs did nothing wrong with voter intimidation
Attorney General Eric H. Holder Jr. called Tuesday for the repeal of laws that prohibit millions of felons from voting, underscoring the Obama administration's determination to elevate issues of criminal justice and race in the president's second term and create a lasting civil rights legacy.
In a speech at Georgetown University, Mr. Holder described today's prohibitions — which in some cases bar those convicted from voting for life — as a vestige of the racist policies of the South after the Civil War, when states used the criminal justice system to keep blacks from fully participating in society.
"Those swept up in this system too often had their rights rescinded, their dignity diminished, and the full measure of their citizenship revoked for the rest of their lives," Mr. Holder said. "They could not vote."
Mr. Holder has no authority to enact the changes he called for, given that states establish the rules under which people can vote. And state Republican leaders made clear that Mr. Holder's remarks, made to a receptive audience at a civil rights conference, would not move them.
Attorney General Eric H. Holder Jr. on Tuesday urged states to repeal laws that prohibit felons from voting, a move that would restore the right to vote to millions of people.
The call was mostly symbolic — Mr. Holder has no authority to enact these changes himself — but it marked the attorney general's latest effort to eliminate laws that he says disproportionately keep minorities from the polls. "It is unwise, it is unjust, and it is not in keeping with our democratic values," Mr. Holder said at civil rights conference at Georgetown University. These laws deserve to be not only reconsidered, but repealed."
African-Americans represent more than a third of the estimated 5.8 million people who are prohibited from voting, according to the Sentencing Project, a research group that favors more liberal sentencing policies. And in Florida, Kentucky, and Virginia, more than one in five African-Americans has lost the right to vote.


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Lindsay Lohan and Samantha Ronson: Madly in Love
Relax, Lindsay Lohan and Samantha Ronson obsessives.
Despite recent relationship troubles and the sight of a lonesome Sam lighting out on her own for Thanksgiving, it appears that everyone's favorite same-sex pair are quite together.
Lohan, 22, and her 31-year-old disc-spinning companion were spotted on Saturday Night at Hollywood's Ecco Lounge, billed as "Hollywood's first eco-friendly ultra lounge."
Well, at least something was friendly that night. SamRo didn't seem too happy. Linds scowled at the assembled paparazzi like they had just run over her puppy, while Sam glowered at the ground in a seeming attempt to will it open so it could swallow her whole.
Careful, you lovebirds; keep frowning like that, and your faces may freeze into that expression. And that could lead to very distressing results indeed.


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Home » Posts tagged with » johnnydepp
aliceinwonderland \ delmar \ DelMarFair \ fair \ johnnydepp \ madaboutthefair \ sandiego \ sandiegocountyfair \ sandiegofair \ sdfair
2016 San Diego County Fair is here!
by Liz Tarnof The San Diego County Fair is back and offers quite a unique outing to anyone's summer plans. This year's fair is all about Alice in Wonderland…aptly dubbed "Mad About the Fair." Guests are drawn into the mad world of Alice where they can enjoy a grand-scale tea party featuring life sized pages straight […]


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This is a very important discussion, because of the possibility of what it could do to this wiki, and what it's about, is simply.
Spoiler warning.
Here's an example, I am at episode 40 of yu yu hakusho, and I knew a certain something was going to happen in the dark tournament saga, but I didn't want to read any comments of yu yu hakusho to know what happened, but when got on this wiki and saw the page, it spoiled what happened to one of the characters, granted I knew but I still wished the page warned about this, rather than just having it on there, like as if the page thought I could see into the future before I even saw the episode of it happening.
So I'm curious if you guys are interested in doing this for the wiki, it's up to you guy.Read more >
i'm just here to help my friends with grammatical errors (രᴗര✿)
Just ignore me~~Read more >
Let's see.
I've been inactive since 2011s. Well, now I'm back, since my jobs have come to an end. I'm sure that I have missed a lot of information here, well, should I start again from beginning? Let's see if there is / are something that I can do...Read more >
Hello guys, I have made an adoption request about adopting this wiki. Once I do get it, we can have a discussion if any of you wants any special rights. Do you approve?
Voting period has ended, please don't comment anymore.Read more >
So, I can type their names and put dash.Read more >
So this is the first time in awhile anyone form Yu yu Hakusho has really been in a game. With Yusuke announced and it seems like Toguro will be announced soon. Is anyone planning on getting the game and what are your thoughts on it. I'll probably get it since im super excited about Yusuke and Togure (and other characters but their not in yuyu hakusho) and the game looks like it will be a lot of fun.Read more >
If Yusuke Urameshi's forefather was a demon, and a human from the start, does that make half-human, half-demon, right? Like, Kurama?Read more >
Their was something on my mind on Minoru Kamiya's territory. The power Mad Doctor, is it the name for his battle style or his territory? Because I'm curious about the name of his territory's name. If anyone has answers, please leave comments about his territory.Read more >
Okay so, Yu Yu Hakusho and Dragonball Z have a lot of things in common. One thing they have in common is buff, strong villains that beat the crap out of people. Who do you think would win, Younger Toguro vs Nappa? Nappa has a power level of about 8,000 and he can survive people self destructing on him and gigantic explosions. Toguro might have a bigger advantage because he can regenerate himself if he loses an arm or is severely injured and don't forget all of his forms and his incredible strength. Nappa has an ability that Toguro doesn't have, he can fly. Who would win? Use logic, guys.Read more >
It seems the Toguro Brothers spirit energy is so powerful, anyone looking at them or sensing them will be terrified. Yusuke was intimidated on how Younger Toguro's power is, the fact he survived Kuwabara's spirit sword (the fact he took the hit) and every time those two make eye contact, Yusuke shakes out of fear. Kuwabara was in shock on how he's still alive and saw he bring down a construction building. Even Hiei was nervous when he senses the brothers spirit energy in Ganzo's house where his sister Yukina was held prisoner.Read more >
If the characters birth are shown, then what year are they born on?
Example:Kaname Hagiri and Minoru Kamiya.Read more >
In the episode "Topside", we seen Kaname alongside his sister. What is his sister's name and what are her powers? The least the directors and editors can do is to give her a name so they don't leave us, the "audience", to wonder.Read more >
I know I'm one of many fan out there who wish that Yu Yu Hakusho could get a video game release on the newer generations of systems. I also know many fans in at least the United States wish to at least get a GOOD YYH games for once, since we didn't get Yu Yu Hakusho Forever. So If I had my say on who I'd want to be playable in this game, which would be a fighter, this is who I'd choose to be in it:
Spirit Dectective Saga
- Yusuke (Base, Spirit Cuffs Unlocked, Demon)
- Kuwabara (Base, Trial Sword, Dimension Sword)
- Hiei (Base, Jagan Form, Dragon Absorbed)
- Kurama (Base, Yoko Kurama)
- Genkai (Old, Young, Masked Fighter)
- Gouki (Base, Demon)
- Kibano
- Musashi
- Rando (Shorin, True Form)
- Genbu
- Byakko
- Seiryu
- Suzaku
- Miyuki
- Gokumonki
- Inmaki
- El…Read more >
It would've been bery interesting if these two fought during the Dark tournament when elder toguro stepped up to fight for the second match but then bui took his place. So i was wondering if elder toguro did fight and hiei also stepped up, who woulda won that fight?Read more >
I noticed on the poll on non-canon pairings that the Hiei/Kurama pairing is leading. I just really want to know why people support this!! I mean, I find this pair really strange even though it was supposed to be incorporated in the series but later cancelled. But a lot of fans seem to support it. In your comments, state if you are for or against this and give your reasons.Read more >
Well, Maybe this topic isn't good, So, Admins, Feel Free to delete.
I Know YYH has been finished, Yoshihiro Togashi-san has done it very well. I believe that some of YYH fans don't want this series over, they want another updates, maybe movies or OVA or another episodes. I have no idea that Yoshihiro Togashi will continue again or not in the future. Well, as fans, I can just hope.
In this post, I would like to ask :
If You heard that Yoshihiro Togashi would continue Yu Yu Hakusho and you were given a chance to share your opinion, What did you wanna see from this new update of Yu Yu Hakusho?
Ok, for me.
First. 3rd Movie. I would love to see it. Maybe in this movie, there was new enemy? It would be a great one!
Second. Character Flashbacks (OVA). …Read more >
Does anyone have a favorite minor antagonist in the series that is not the main antagonist of an arc? (Thus no YoungerToguro, Sensui, or the Kings). Multiple characters if possible. Any enemy from before the dark tournament (other than Toguro) would count, becuase it was a much smaller scale then. Also, try to avoid the six reformed demons or Genkai's three students, I'm trying to see if you liked any of the characters that were minor enemies in the dark tournament like Gama or Risho (Even high ranking enemies who weren't the main villain like Karasu and Itsuki would count), though I guess enemies in the Sensui saga other than the Dark Angel count as well.
Zeru - this guy always seemed really badass to me, he had a variety of interesting wa…Read more >
Hehe. Since I'm new here, I'm still learning for a bit how this wiki works. Well, I think I'll begin the first topic... And It's general topic that asked by lot of YYH fans. What are your favorite character? (one hero and one villain please + Reason). And since I still don't get it for 100%, feel free to tell me that I have to edit it if there are some mistakes ;D
Ok. Start by myself :
Hero : Yusuke Urameshi
Reason : Urm... Well, Basically... I like his techniques, Spirit Gun, Spirit Shotgun, and Spirit Cuffs. And yeah, He seems encourage and inspire me how to care each other and make a good relation with others. And about his look, well, I think he's perfect for anything. And that's the way I think about him.
Villain : younger toguro
Reason : …Read more >
Hello, I'm felicia. since I'm still new here, I know nothing. And I'm here to help you to give summary of YYH. I'm YYH big fan since 2001s. I'm from Indonesia. I'm sure 100% that my English won't be perfect as you guys here. So, If you find my English in a mess, you are allowed to edit it.
And sometimes, I will ask several people here how to use this Wiki.
That's all my introduction. More about me, you can leave me a message.Read more >
Which particular technique do you think is the best in the series? All characters can be taken into consideration.
I think it should be Hiei's dragoon of the darkness flame. It's one of the most powerful techniques requiring tremendous power to control and when wielded correctly it can obliterate almost anything.
Leave your favorite in the comments!Read more >
So, who do you think is the most annoying/worst character in the series?
Read more >
Mine might be Rinku because his voice is annoying (and unconvincing) and how out of place he seems with the rest of the ally demons in terms of appearance and abillities. Also, DUB Yukina, as while i liked her in the manga and Jap anime as an antithesis to the typical evil, corrupt demon (she wasn't evil at any point, unlike the reformed Kurama), but in a series where the dubbed main cast had stellar voice acting, she stood out like a sore thumb (i hate how her bored sounding voice ruined the scene in which she begs Hiei to not kill Tarukane)
If YYH would continue, would you want it too?
Personally, I don't
It was great, and it was short (for big name shonen series standards). All the seasons seemed to play a role in the overall series, with filler arcs removed in the anime (another reason why i prefer the anime). Any extra seasons would seem extraneous
If there is any continuation, maybe a movie (maybe showing Koenma taking over Spirit World like in the manga against his father) or an epilogue episode that showed what happened to everybody, once Yusuke came back (after Eizou).
However, an entire series would not be something I look forward to. I mean there probably would be some contrived reason why humans and demons would have to fight again.
I mean, look how Hunter x Hunter turne…Read more >
Read more >
You are encouraged to state your reasoning/opinions below!
Which of the two do you prefer or do you like both. Such as the Yusuke's time as a ghost is longer with far more adventures in Volumes 1 and 2 of the manga, or that the Three Kings saga has a larger amount of material in the anime, including the showing of numerous tournament matches.Read more >
At first, I kind of thought that the ability to fly in Yu Yu Hakusho would only be possessed by demons such as Jin, you know, people who are able to manipulate wind.
However, in the Demon World Tournament, not only did I find out that flying isn't only a Wind Master's power, but there are others who can do it better than them! Souketsu, one of Raizen's old friends beat Jin in a "race" to the sky. And not only that, Jin did not even know that Souketsu had even taken flight! "That's the Wind Manipulator's tragic flaw. You cannot exceed the speed of the wind itself."
So I suppose that means that Jin isn't able to fly faster than the speed of the wind.
But, that leaves the question, how did Souketsu fly? And not only that, Shuu, another of Raizen…
Read more >


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|By Brixzeen
|See other fanon and fan fiction works from Brixzeen.
|Biographical information
Metal Clan Citizen
|Birth place
Eastern Air Temple
Southern Water Tribe
|Physical description
|Hair color
Dark Brown
|Skin color
|Eye color
|Personal information
|Weapon of choice
All Elements
|Fighting style(s)
Wooden Fans
Energy Restoration
|Chronological and political information
Amai was born after Korra's quiet death in the Southern Water Tribe, she was born in the Eastern Air Temple to single mother, Chi. Chi was a former citizen of Zaofu, but then fled the city and resided in the Eastern Air Temple as an Acolyte. Once Amai was born, she showed some signs of being the Avatar, but when the Earth Nation was fully occupied by other nations looking for the Avatar, her mother had her hidden from the world. Once she reached the age of 11, she fled from the Air Temple Islands and found herself at a place she should have never been at.
Finding herself on a deserted island in the middle of no where, she found out the she got stuck in the middle of the ocean. While trapped, Avatar Korra visited her and talked to her. Amai had the same doubts about her power and ignored Korra, she wanted to learn on her own about the world.
Amai sailed the oceans on a raft she constructed. Only having the ability to bend earth, she was powerless on the ocean. She traveled for days, only satisfying her needs with coconuts she piled up. In time, she found herself on a swamp by the Earth Kingdom. Korra, being one of her past lives recognized the swamp as the place she met Toph Beifong. Still, Amai ignored Korra and continued inside the swamp. She witnessed some swamp benders moving the vines, and she thought about how could they bend the water within. Korra attempted to talk to her once more, but she refused and screamed. Alarmed by the voice, the swampbenders took immediate actions, but Amai hid herself by earthbending rocks to cover her.
Once the swampbenders were gone, she got up and dusted herself. She walked into the swamp and saw a huge tree. Feeling the power of the tree, Amai was drawn into it and sat down by the tree. Like Toph, Amai felt the world within, she could feel all sorts of places, and in mere seconds, she found her spirit all over the world. Korra sat by her side and told her how she was amazed about how the world was, but Amai ignored her again. Korra, now infuriated asked her what was her problem, Amai quietly told her that she wanted to learn not from philosophy shoved down her by other people, but from what she could see. Korra just told her that she should follow her heart and then disappeared. Amai transferred from the Earth Kingdom to Republic City, then to the Eastern Air Temple, where her mom was looking for her.
Her exploration was then interrupted by a conversation between two swamp people. One was complaining about his job and the other told him how easy his job was considering he was a bender. The bender told him that he was just jealous, and threatened to hurt him. Amai came out of hiding and tried to help, the swamp bender noticed her and commanded the vines to entangle her. Once she was tied up, she was about to enter the Avatar State, but declined the use of it. She didn't want any knowledge. Unable to access any power, Amai struggled for the last time and broke free, having no access rather than earth bending, she was beaten by the swamp bender. Upon the brink of being knocked out, she blasted a green beam and the man was stripped of a green essence. Now at her palms was a green energy, the other swamp man tried to run, but Amai redirected the ball towards him and he glowed with a light green aura.
She thought that what she did was wrong, and tried to help the man. The man stood up and told her he felt fine. The swamp bender stood up and then tried to bend the vines near him, but couldn't. He thought his bending was gone, but bended water once Amai tried to hit him with a boulder. The other man was confused upon hearing this, and accidentally tripped but before landing on the ground, he was comforted by vines and plants. He thought he became a water bender and was overjoyed. But when he tried to bend water, he failed. He then bended the vines again, and it worked. Amai was astounded.
See more
For the collective works of the author, go here.


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Genome-wide analysis of the MYB transcription factor superfamily in soybean
Genomics and evolution
The MYB superfamily constitutes one of the most abundant groups of transcription factors described in plants. Nevertheless, their functions appear to be highly diverse and remain rather unclear. To date, no genome-wide characterization of this gene family has been conducted in a legume species. Here we report the first genome-wide analysis of the whole MYB superfamily in a legume species, soybean (
A total of 244 R2R3-MYB genes were identified and further classified into 48 subfamilies based on a phylogenetic comparative analysis with their putative orthologs, showed both gene loss and duplication events. The phylogenetic analysis showed that most characterized MYB genes with similar functions are clustered in the same subfamily, together with the identification of orthologs by synteny analysis, functional conservation among subgroups of MYB genes was strongly indicated. The phylogenetic relationships of each subgroup of MYB genes were well supported by the highly conserved intron/exon structures and motifs outside the MYB domain. Synonymous nucleotide substitution (
In this study we identified the largest MYB gene family in plants known to date. Our findings indicate that members of this large gene family may be involved in different plant biological processes, some of which may be potentially involved in legume-specific nodulation. Our comparative genomics analysis provides a solid foundation for future functional dissection of this family gene.
Transcription factors are usually composed of at least four discrete domains: a DNA-binding domain, a nuclear-localization signal, a transcription-activation domain, and an oligomerization site. These domains operate together to regulate many physiological and biochemical processes, and to activate and/or repress transcription in response to endogenous and exogenous stimuli
MYB transcription factors are widely distributed in all eukaryotic organisms, and constitute one of the largest transcription factor families in the plant kingdom. MYB proteins are defined by a highly conserved MYB DNA-binding domain at the N-terminus
The first identified MYB gene was the v-
Presently, most of our knowledge of plant MYB genes was obtained from studies of the major genetic model plant,
Given the potential roles of MYB proteins in regulation of gene expression, secondary metabolism, and responses to environmental stresses, and that soybean is the first legume for which the complete genome has been sequenced, it is of interest to determine how many MYB genes exist in this species, as well as the characteristics of gene structures, phylogenetic relationships, chromosome locations, conserved motifs and expression patterns. Moreover, it is important to unravel the functionality of new and as yet uncharacterized soybean MYBs, especially those that might be involved in seed development and legume-specific nodulation. Within this framework, in the present study we identified the entire complement of MYBs in soybean and conducted a genome-wide comparative analysis of MYBs between soybean and
Results and discussion
Identification and classification of the soybean MYB gene family
With the aim of defining the soybean MYB gene family, we searched the entire soybean genome sequence for genes that encode proteins containing the MYB DNA-binding domain. BLASTP searches were performed using consensus-typical R2R3-MYB DNA-binding domain sequences as queries. We identified approximately 700 sequences that contain MYB or MYB-like repeats; these represented approximately 1.7% of the 46,430 predicted soybean protein-coding loci, and 14% of the 5671 putative soybean transcription factor genes
In order to verify the reliability of our results, we performed PROSITE profile (PS50090) and simple modular architecture research tool (SMART) analysis to identify all of the putative MYB protein sequences in the soybean genome. We excluded from further analysis 26 putative MYB protein sequences that contained typical R2R3-MYB domains but lacked partial sequences in the N-terminal region (
Additional file 1
Summary of the MYB transcription factor genes in soybean.
Click here for file
Multiple sequence alignment and sequence features of the MYB domains
To investigate the homologous domain sequence features, and the frequency of the most prevalent amino acids at each position within each repeat of the soybean R2R3-MYB domain, we performed multiple alignment analysis using the 244 homologous domain amino acid sequences of R2 and R3 repeats (Figure
Figure 1
R2 and R3 MYB repeats are highly conserved across all R2R3-MYB proteins in the soybean genome
R2 and R3 MYB repeats are highly conserved across all R2R3-MYB proteins in the soybean genome. The sequence logos of the R2 (a) and R3 (b) MYB repeats are based on full-length alignments of all soybean R2R3-MYB domains. Multiple alignment analysis of 244 soybean-typical R2R3-MYB domains was performed with ClustalW (for full representation of the alignment, see
In general, the basic regions of MYB domains had 108 basic residues (including the linker region), with rare deletions or insertions (approximately 6%;
Additional file 2
Alignment of all MYB proteins used in this study.
Click here for file
Sequence similarity among the 244 GmMYBs at the cDNA level showed a high degree of sequence divergence ranging from 5.50% to 99% in the open reading frame (ORF). The highest similarity in the ORF was observed between GmMYB100, GmMYB243, and GmMYB244, whereas the lowest similarity was observed between GmMYB029 and GmMYB156. Most of the genes had very short 5′-untranslated regions (UTR) (Figure
Additional file 3
The complete version of Figure 2: Phylogenetic relationships and subgroup designations in MYB proteins from soybean (Gm),
Click here for file
Figure 2
Phylogenetic relationships and subgroup designations in MYB proteins from soybean (Gm),
Phylogenetic relationships and subgroup designations in MYB proteins from soybean (Gm),
As an indicator of the selective pressures that act on these genes, we calculated ratios of nonsynonymous vs synonymous nucleotide substitutions (
Alternative splicing of the MYB gene
Alternative splicing (AS) is the mechanism by which a common precursor mRNA produces different mRNA variants, by extending, shortening, skipping, or including exon sequences, or retaining intron sequences. This allows production of many gene products with enriched functions from a single coding sequence. Recently, it was proposed to be a mechanism by which higher-order diversity is generated, thereby leading to a diverse population of RNAs
About 27% of 18,933 transcription units in rice genomes contain two or more transcripts because of AS
In general, these AS events resulted in a variety of sequence insertions and/or deletions in the corresponding ORFs. For instance, a 33 bp alternative 3′ splice site in GmMYB028 allowed the lengthening or shortening of 11 amino acids in the R3 repeat. Similarly, a 21 bp alternative 3′ splice site in GmMYB140 resulted in a deletion of seven amino acids in the R3 repeat. Interestingly, we observed that some of the AS events changed the type of MYB protein. For example, a 189 bp alternative promoter site of GmMYB082 resulted in a frame shift, which changed the R2R3-MYB into a signal-repeat MYB type. Similarly, a 237 bp alternative promoter site in GmMYB152 that transposed the 5′ exons changed the typical R2R3-MYB gene into a signal-repeat MYB gene. In contrast, GmMYB237 exhibited three alternative types of splicing by alternative promoters and alternative poly(A) sites. The first transcript resulted in a deletion of more than 2000 bp at the 5′ terminus and a long insertion at the 3′ terminus; the second resulted in a long deletion at the 3′ terminus; and the third (3R-MYB protein) resulted in a deletion of the R1 and R3 repeats in the 3R-MYB protein. In
There are currently two models for the evolution of the MYB DNA-binding domain: the 'gain' and the 'lose' models. The 'gain' model proposes that the R1R2R3-MYBs are generated by successive intragenic duplications or triplications in primitive eukaryotes
Phylogenetic analysis of the MYB family genes
To evaluate the evolutionary relationships within the MYB gene family, we performed a combined phylogenetic analysis of
Additional file 4
MP phylogenetic tree of 387 MYB proteins in soybean,
Click here for file
The observed sequence similarity and phylogenetic tree topology allowed us to classify the MYB genes of soybean,
In comparisons of the multigene families, the MYBs from the same lineage tended to be clustered together in the phylogenetic tree, which suggested that they were duplicated after the divergence of the lineages. Moreover, soybean and
However, it is difficult to infer orthologs merely from tree topologies. In general, the gene functions of a clade appear highly but not absolutely conserved across plant species. Therefore, it is useful to accurate identify the true orthologs between plant species, based on syntenic relationship. To support the putative orthology of GmMYBs and AtMYBs, we examined the syntenic relationship based on gene content, order, and orientation between the genomes of soybean and
Additional file 5
Synteny blocks of
Click here for file
Definition of intron/exon structures within the MYB domains
The observed intron distribution, positions, and phases within each subfamily are shown in Figure
Figure 3
Schematic diagram of intron distribution patterns within the MYB domains of soybean MYB (GmMYB) proteins.
Schematic diagram of intron distribution patterns within the MYB domains of soybean MYB (GmMYB) proteins. Alignment of MYB domains is representative of 14 intron patterns, designated a to n. Locations of introns are indicated by white triangles, and the number within each triangle indicates the splicing phases of the MYB domain sequences: 0 refers to phase 0; 1 to phase 1; and 2 to phase 2. The number of GmMYB proteins with each pattern is presented on the right. The correlation of intron distribution patterns and phylogenetic subfamilies is provided in Figure
It was previously reported that most
Remarkably, genes in the same subfamily generally showed the same intron pattern, with the position of the intron almost completely conserved within most subfamilies. This finding constitutes an independent criterion for testing the reliability of our phylogenetic analysis. For example, the subfamily C42 lacked introns 1 and 2; subfamily C5 lacked intron 2; subfamily C30 lacked intron 1; and subfamily C4 retained the typical splicing sites (Figure
We further observed that the splicing sites of introns were generally located in the R2 and R3 repeats, and that the amino acids close to the splicing sites were highly conserved (Figure
In addition to splicing sites, we investigated the intron phase in the MYB domain with respect to codons. The splicing of each intron is thought to occur in one of three phases: phase 0, phase 1, or phase 2, depending on whether the splicing occurs between codons. In phase 0, splicing occurs after the third nucleotide of the first codon; in phase 1, splicing occurs after the first nucleotide of the single codon; and in phase 2, splicing occurs after the second nucleotide
The exon length of the MYBs was highly conserved within each subfamily. Exons 1 and 2 appeared to be more restricted in length, whereas exon 3 was the most diverse in size. In general, exons 1 and 2 were very similar in length (exon 1, 133 bp; exon 2, 130 bp) and were also highly conserved (exon 1, 27% occurrence; exon 2, 68% occurrence). Exon 3 coded the last region of the R3 repeat and the C-terminal regions of the MYB protein. The diverse length of this exon could facilitate the gaining of new or cooperative functional motifs and domains, which may account for the functional divergence between MYB homologs in plants. Interestingly, the exon lengths observed for the R2R3-MYB superfamily in soybean coincided with those documented for
Strikingly, in terms of intron position, length and phase, the soybean gene structures revealed in the present study were consistent with the phylogenetic subfamilies of
Chromosomal distribution and duplication events among soybean R2R3-MYB genes
To determine the genomic distribution of the MYBs, we searched the soybean genome database using the DNA sequence of each soybean MYB gene and BLASTN. Genome chromosomal location analyses revealed that soybean MYBs were distributed on almost all chromosomes. Although each of the 20 soybean chromosomes contained MYBs, the distribution appeared to be uneven (Figure
Figure 4
Chromosomal locations, region duplication, and predicted cluster for soybean MYB genes.
Chromosomal locations, region duplication, and predicted cluster for soybean MYB genes. Chromosomal positions of the MYB genes in soybean are mapped on the basis of JGI soybean Genome version 7.0. The chromosome number is indicated above each chromosome. The scale is in megabases (Mb). The number below the chromosome name indicates the length. The phylogenetic category of each gene (Figure
Gene duplication has long been recognized to occur throughout plant evolution, thereby contributing to the establishment of new gene functions and underlying the origins of evolutionary novelty
In the present study, intrachromosomal duplication was also observed in the soybean genome. A series of tandem gene duplications was also indicated to contribute to increasing the number of R2R3-MYB family genes, as evidenced by the fact that some closely related GmMYBs in a single cluster were physically located near to each other on a given chromosome with no intervening annotated gene: for example, GmMYB099/GmMYB226 at the bottom of chromosome 3, GmMYB031/GmMYB112 at the top of chromosome 10, and GmMYB026/GmMYB043 at the top of chromosome 19 (Figure
It is likely that different MYBs evolved after duplication of their DNA-binding domain, subsequently by a series of synonymous and/or non-synonymous mutations in the ORF (especially in the C-terminal region), to generate new functions during evolution. Together, our findings indicate that entire genome duplication, segmental duplication, and tandem gene duplication all contributed to R2R3-MYB gene expansion in soybean. The number of tandem duplications was much fewer than the number of genome and/or segment duplications, therefore the latter might have been the main contributor to MYB gene expansion in soybean.
Conserved motifs outside the MYB domain
Interestingly, the relative spatial location of the plant MYB domain is variable, and may be in the N-terminal, middle, or C-terminal regions of the protein. In typical R2R3-MYB proteins, the MYB domain is usually located close to the N-terminal region, and the very short region before the MYB domain appears to be less conserved between different subfamilies. Consequently, no motifs have been detected in these regions throughout the plant MYB gene family. By contrast, C-terminal regions outside the MYB domain generally contain many specific, functionally important domains or motifs involved in transcriptional activity and protein–protein interactions. Proteins within a subfamily that share the same motifs are likely to share similar functions.
Using MEME, we searched for conserved motifs in the soybean MYB proteins of each group. Most members of a subfamily shared one or more motifs outside the MYB domain, which provided further support for definition of the subfamily. We identified a total of 50 conserved motifs in the C-terminal regions (Table
The schemes of protein motifs of individual members of the MYB family revealed the structural similarities among proteins within subfamilies (Figure
Expression pattern of R2R3-MYB genes in soybean and
Transcription factor genes comprise a substantial fraction of all eukaryotic genomes. On the basis of the type of DNA-binding domain that they encode, the majority of transcription factor genes can be grouped into a handful of different, often large, families. Functional redundancy is not unusual within these families; thus, the proper characterization of a particular transcription factor gene often requires its study in the context of a whole family. By forming intricate networks through protein–protein interactions, and also at the transcriptional level, transcription factors control the expression of the genome. Ultimately, therefore, their functions cannot be understood without considering their activities at a genome-wide scale. It has been noted previously that many transcription factor gene families exhibit great disparities in abundance among different eukaryotic organisms, and that some families are lineage specific.
The recently developed RNA-Seq web-based tools, which include gene expression data across multiple tissues and organs, allow characterization and comparison of the gene transcriptome in soybean
In common with genes that encode transcription factors, many of the GmMYBs exhibited low transcript abundance levels, as determined by the RNA-Seq Atlas analysis. After integration of the two data sets, we observed that most of the genes had very broad expression spectra. However, five AtMYBs and 32 (13%) GmMYBs lacked expression information, which possibly indicated that these were pseudogenes or were expressed only at specific developmental stages or under special conditions. Next, we examined data from a second soybean transcriptome atlas
We observed that accumulation of R2R3-MYB gene transcripts was associated with different tissues, and the expression pattern of each R2R3-MYB gene member differed (Figure
Although the expression pattern of MYBs varied between soybean and
Transcription factors are not only involved in the activation of plant defense systems, but also play key roles in the control of mutualistic interactions between plant roots and soil microorganisms. For example, nodulation involves the interaction between root and soil bacteria, leading to symbiotic nitrogen fixation, which is mainly restricted to legumes. The first transcription factor shown to be involved in nodulation was the
Given that the soybean MYB gene family is so large, a key question is whether the various paralogs show similar expression patterns suggestive of functional redundancy. As shown in Figure
Putative functions of soybean R2R3-MYB transcription factors
Phylogenetic analysis allowed identification of putative orthologous and paralogous MYBs. It was suggested previously that orthologous genes usually share similar functions and are clustered in the same clades and subclades, whereas paralogous genes generally display different functions, which indicates that closely related MYBs can recognize similar target genes and may possess functional redundancy. Thus, analysis of orthologous and paralogous genes is essential to comparative genomics.
Currently, the most extensive annotative evaluation of plant MYBs was obtained from studies of
In general, most plant MYBs are positive regulators of transcription. For example, AtMYB12 activates the phenylpropanoid biosynthetic pathway by up-regulating the expression of chalcone synthase (
Combinatorial interactions between transcription factors are pivotal to the regulation of eukaryotic gene expression. The combinatorial regulation of the flavonoid biosynthetic pathway by the bHLH-MYB-WD40 complex is well characterized. These interactions can either modulate transcription factor activity or contribute to the biological specificity of factors with very similar DNA-interaction motifs. In maize, the MYB protein C1/Pl was reported to depend on the interaction with R/B (bHLH protein) for its regulatory function in anthocyanin biosynthesis
The 3R-MYB genes are present predominantly in vertebrates; however, members of this three-repeat MYB gene family have been detected also in some plant lineages, including
MYB genes are widely distributed among higher plants and comprise one of the largest groups of transcription factors in the plant kingdom. However, the specific roles of most plant MYB genes remain unknown. In the present study, we attempted to elucidate characteristics of soybean MYB gene structures, phylogenetic relationships, chromosomal locations, conserved motifs and expression patterns, as well as their functional diversification, by identifying the probable full complement of GmMYB genes in soybean. We performed extensive analyses of the candidate soybean MYB genes and compared them with those of
We classified the soybean and
In addition, comparative expression profile analysis of MYBs in soybean and
Database search for MYB proteins in soybean and
Gene identifiers for 126
On the basis of the results of BLASTP searches in the soybean genome database of JGI (using the predicted proteins of soybean MYB genes), we obtained information on cDNA sequences, genomic sequences, intron distribution patterns, phases, and intron/exon boundaries. The centromeric and pericentromeric regions of soybean chromosomes and the synteny within soybean genome were detected by JGI soybean browser (
Multiple sequence alignments
Using the Multalin tool (
Nucleotide substitutions levels were calculated using the alignment of MYB domain of GmMYB proteins by Hyphy version 2.0
Additional file 6
NJ phylogenetic tree of 244 soybean R2R3-MYB proteins.
Click here for file
To compare the evolutionary relationships of soybean and
Protein motif identification
In order to investigate protein motifs in more detail, we used the MEME version 3.5.7 tool to identify conserved motifs shared among MYB proteins
Phylogenetic analysis
To explore the evolutionary relationships of soybean and
In this study, to identify the orthologs, we defined synteny blocks to include conservation of gene content, order and direction between different chromosomes and/or genomes. The cross-genome syntenic relationships between
Expression analysis of AtMYBs and GmMYBs
We used the Soyseq (
Authors' contributions
HD carried out the genome search, sequence analysis, phylogenetic analysis, interpreted the results and drafted the manuscript. BF, together with HD, contributed to the genome search and compiled the sequence alignments. SY and LL contributed to the phylogenetic, expression and structure analyses. ZL carried out the synteny, and nucleotide substitution analyses. YH and YT jointly contributed to the conception and coordination of the study, were involved in revising the manuscript, and gave final approval of the version to be published. All authors read and approved the final manuscript.
This project was supported by the State Key Basic Research and Development Plan of China (2011Cb100100 and 2011Cb100106), the National Natural Science Foundation of China (31071354), and the National Transgenic Program (2009ZX08009055B).
Arabidopsis transcription factors: genome–wide comparative analysis among eukaryotes
One billion years of bZIP transcription factor evolution: conservation and change in dimerization and DNA-binding site specificity
One billion years of Myb
The R2R3–MYB gene family in Arabidopsis thaliana
Comparison of the free and DNA–complexed forms of the DNA–binding domain from c–Myb
Towards functional characterisation of the members of the R2R3–MYB gene family from Arabidopsis thaliana
Multifunctionality and diversity within the plant MYB–gene family
Nucleotide sequence of the retroviral leukemia gene v–myb and its cellular progenitor c–myb: the architecture of a transduced oncogene
MYB transcription factors in plants
Molecular evolution of the Myb family of transcription factors: evidence for polyphyletic origin
Myb proteins in life, death and differentiation
The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto–oncogene products and with structural similarities to transcriptional activators
EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesis
The Arabidopsis transcription factor MYB12 is a flavonol–specific regulator of phenylpropanoid biosynthesis
Conifer R2R3–MYB transcription factors: sequence analyses and gene expression in wood–forming tissues of white spruce (Picea glauca)
Differential regulation of closely related R2R3–MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling
Long-day up-regulation of a GAMYB gene during Lolium temulentum inflorescence formation
Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling
Transcriptional repression by AtMYB4 controls production of UV–protecting sunscreens in Arabidopsis
Differential combinatorial interactions of cis–acting elements recognized by R2R3–MYB, BZIP, and BHLH factors control light–responsive and tissue–specific activation of phenylpropanoid biosynthesis genes
Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis
AtMYB103 regulates tapetum and trichome development in Arabidopsis thaliana
Development of three different cell types is associated with the activity of a specific MYB transcription factor in the ventral petal of Antirrhinum majus flowers
Control of cell and petal morphogenesis by R2R3 MYB transcription factors
Functional analysis of the epidermal–specific MYB genes CAPRICE and WEREWOLF in Arabidopsis
c–MYB oncogene–like genes encoding three MYB repeats occur in all major plant lineages
The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family
Male germ line development in Arabidopsis: duo pollen mutants reveal gametophytic regulators of generative cell cycle progression
Expansion and Diversification of the Populus R2R3–MYB Family of Transcription Factors
RNA-Seq Atlas of Glycine max: a guide to the soybean transcriptome
Genome sequence of the palaeopolyploid soybean
Identification of conserved gene structures and carboxy–terminal motifs in the Myb gene family of Arabidopsis and Oryza sativa L. ssp. indica
Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality–related clades and conserved gene structure organization across Vitis and Arabidopsis genomes
Alternative splicing and genome complexity
Prediction of alternative isoforms from exon expression levels in RNA-Seq experiments
Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice
A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome
Alternative isoform regulation in human tissue transcriptomes
Ordered origin of the typical two- and three-repeat Myb genes
Maize R2R3 Myb genes: sequence analysis reveals amplification in the higher plants
Newly discovered plant c-myb-like gene rewrite the evolution of the plant myb gene family
MYB transcription factors in Arabidopsis
A Systems Biology Approach Identifies a R2R3 MYB Gene Subfamily with Distinct and Overlapping Functions in Regulation of Aliphatic Glucosinolates
The transcription factor HIG1/MYB51 regulates indolic glucosinolate biosynthesis in Arabidopsis thaliana
HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in Arabidopsis thaliana
Speculations on RNA splicing
Evolution's cauldron: duplication, deletion, and rearrangement in the mouse and human genomes
The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana
An integrated transcriptome atlas of the crop model Glycine max, and its use in comparative analyses in plants
A plant regulator controlling development of symbiotic root nodules
Coordinating nodule morphogenesis with rhizobial infection in legumes
The HCL gene of Medicago truncatula controls Rhizobium-induced root hair curling
Medicago truncatula NIN is essential for rhizobial-independent nodule organogenesis induced by autoactive calcium/calmodulin-dependent protein kinase
Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators
Positional cloning identifies Lotus japonicus NSP2, a putative transcription factor of the GRAS family, required for NIN and ENOD40 gene expression in nodule initiation
The novel symbiotic phenotype of enhanced-nodulating mutant of Lotus japonicus: astray mutant is an early nodulating mutant with wider nodulation zone
Expression profiling in Medicago truncatula identifies more than 750 genes differentially expressed during nodulation, including many potential regulators of the symbiotic program
An ERF transcription factor in Medicago truncatula that is essential for Nod factor signal transduction
EFD is an ERF transcription factor involved in the control of nodule number and differentiation in Medicago truncatula
A positive regulatory role for LjERF1 in the nodulation process is revealed by systematic analysis of nodule-associated transcription factors of Lotus japonicus
A plant receptor-like kinase required for both bacterial and fungal symbiosis
A novel ARID DNA-binding protein interacts with SymRK and is expressed during early nodule development in Lotus japonicus
Large-scale analysis of putative soybean regulatory gene expression identifies a Myb gene involved in soybean nodule development
PcMYB1, a novel plant protein containing a DNA-binding domain with one MYB repeat, interacts in vivo with a light-regulatory promoter unit
AtMYB32 is required for normal pollen development in Arabidopsis thaliana
The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco
TT8 controls its own expression in a feedback regulation involving TTG1 and homologous MYB and bHLH factors, allowing a strong and cell–specific accumulation of flavonoids in Arabidopsis thaliana
TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana
Cloning and molecular characterization of R2R3–MYB and bHLH–MYC transcription factors from Citrus sinensis
Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor
Identification of the residues in the Myb domain of maize C1 that specify the interaction with the bHLH cofactor R
Conservation and diversification of three–repeat Myb transcription factors in plants
R1R2R3–Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana
Myb controls G(2)/M progression by inducing cyclin B expression in the Drosophilaeye imaginal disc
SMART 4.0: towards genomic data integration
The InterPro database, an integrated documentation resource for protein families, domains and functional sites
Multiple sequence alignment with hierarchical clustering
Genedoc: analysis and visualization of genetic variation
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools
HyPhy: hypothesis testing using phylogenies
MEME: discovering and analyzing DNA and protein sequence motifs
Combining evidence using p–values: application to sequence homology searches
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0
Synteny and Collinearity in Plant Genomes
The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses


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|Reviews for Where Loyalty Lies
| the sacred night chapter 3 . 3/30/2004
YAY more story! i like howyou kept it sort of parallel to the manga storyline, but not entirely. i have a question: can Shifters assume any shape they want, or do they just have a second form they can change into and nothing else? i just wondered because kenshin was changed into that winged red batlike creature both times, but then he changed his eye color. things like this inerest me, and in my fic, he's a vampire, and they can chage shape as well. he makes an excellent vamp! you wouldn't believe all the species people make him... one author even made up a species.
| Riverwood chapter 2 . 1/4/2004
Whoo! Nice. Good job good job good job! _ I wana know more about these Shifter thingies... they're kewl! Or at least Kenshin is... *drool...* Kenshin... . ;
Oh, they're on another planet? Yay! Sci-Fi! *clap clap clap* Sci-Fi's da best!
Ya' want some Arizona Green Tea?
| unique-starfish chapter 1 . 1/1/2004
problem: okay...i was a bit confused when i saw your prologue posted in the erm...part where 'strangers on the road' should be, but when i went to the part where the prologue should have been...well...it turned out to be your first chapter...make sense? what i mean is, you have your two chapters out of order...try and fix it, okies? _
okay, onto my review. i liked this chapter...it was written well (as usual), and it's getting very interesting...
oh, and yeah! _ i found your ficcy! _
| the sacred night chapter 1 . 12/27/2003
very interesting! you're right, i don't really know who these people are, though i have suspicions. i like it a lot, though, and even if it wasn't rk i would read it! i like the winged person.
| unique-starfish chapter 1 . 12/26/2003
You are a really good writer - and there's no doubt about it! I really do love this so far! (and I guess you could say this was short, but then my chapters are always short...o_O) Update soon! _
| Riverwood chapter 1 . 12/26/2003
Oh, wow! Nice use of big words! _~ And a kewl prologue, too! I wonder who these two are... lemme guess... the red one's Kenshin and the person driving the car is Kaoru? I dunno who the mechanical thang is, tho. At first I thought it might be Sano, but that would be... *really* weird... O_o;
Keep it up!
| Crazy Girl Person chapter 1 . 12/25/2003
Very interesting prologue. Keep it up!


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How to Choose the Right Influencer for Your Brand
A recent study by Tomoson found that influencer marketing is the fastest-growing online customer-acquisition channel, ahead of organic search, paid search and email marketing. Here are some tips for how to choose the right influencer for your brand.
A good spread of followers on a variety of platforms is desirable, rather than influencers only present on one platform such as Instagram, with no blog. This will ensure greater visibility and longer lasting impact in terms of Search Engine Optimization. Moreover, in the Tomoson study, marketers ranked the following social media platforms by order of effectiveness:
1) Blogs
2) Facebook
3) Instagram and YouTube
A large number of followers doesn't mean much without a correspondingly high level of engagement – e.g. comments on an Instagram post or retweets on Twitter.
Choose an influencer with a reader/follower profile that matches your brand. A fashion influencer with a predominantly teenage audience may not be as well suited to a luxury brand as a more specific luxury influencer with readers/followers mainly aged over 25 and in the A & B income groups.
The best influencers generally create their own content and photos, rather than regurgitating press releases. Not only can using duplicate content have severe consequences for your brand, but readers will be turned off by the lack of personal opinion.
Make sure that the influencer is using their own photos – it's not uncommon to find photographs simply lifted from an unsuspecting photographer's site, with no credit whatsoever. Where they have used someone else's photos, there should be a clear credit unless these are free for commercial use.
The influencer's written and spoken English should be perfect, with no grammatical or spelling mistakes that could reflect badly on your company.
Authority can be measured in a number of ways. Domain authority, for example, affects how the content on sites is ranked by search engines. You can check a site's domain authority at Moz Open Site Explorer. A domain authority over 30 is considered desirable by some brands. Try to choose an influencer who is considered an expert in their field and well known within the blogging community.
Hopefully you've found these tips helpful, it's a vast subject. We do assist brands in identifying key influencers for their projects so please get in touch if you'd like to find out more about this and about how to work with influencers once you've found them.


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