text:
Web ID: 90150
Here is a large image and some of the thumbnails. The tittle of this image is Custom bedroom furniture set with image of bedroom furniture decor on design. Design for deluxe bedroom furniture in minimalist white bedroom ideas. Simple bedroom design with wooden furniture wooden set on dark wood flooring. Spacious platform dark bedroom idea furniture sets in grey interior decor. Traditional bedroom design inspiration with brown solid wood bedroom furniture.
The large picture on this section is grouped by bedroom furniture set.


text:
Sturdy electric stapler is designed for repeated commercial use with both durability and high-quality in mind. Provides reliable stapling performance each day.
Watch video
To compare the product you selected, please choose a product below to replace
View compare or close and continue browsing
Sign up to receive the latest Swingline news, solutions & offers
Thank you
© 2017 ACCO Brands. All Rights Reserved.

subset: commoncrawl


text:
PolyCase's Sport Utility Ammo is the reinvention of their original Inceptor RNP/TNP line. PolyCase has taken a second look at the specifications of their standard line and revamped their bullet weight, velocity and price to make an ideal "plinking" round. Just like the original RNP/TNPs, the Sport Utility rounds shoot and cycle similarly to PolyCase's defensive and hunting ARX line, making them the ideal training rounds for those who hunt or carry the ARX. As with all of PolyCase's bullets, these are non-expanding, injection molded projectiles formed by a copper-polymer composite built for high speeds and low recoil.
This is loaded ammunition.
Caliber: .38 Special
Weight: 84 gr.
Bullet: Copper-Polymer RNP
Velocity: 1210 fps
Energy: 273 ft. lbs.
About the Manufacturer:
PolyCase Ammunition was founded on the idea of incorporating polymers into the components of ammunition. As their name suggests, their flagship product is polymer-cased ammo that weights significantly less than brass or nickel-cased rounds. PolyCase's currently available Inceptor line includes their self-defense ARX round, Firefly Tracer round and a standard Round Nose Point. All of PolyCase's projectiles are injection molded from a copper-polymer mix and are lighter than comparable lead rounds.
$ 19.99
PolyCase's award winning Inceptor ARX ammo is now available in .38 Special. The ARX is designed to transfer maximum energy to target by a hydrodynamic ram effect. The unique shape of the ARX bullet enhances the surface area and exploits the rotational force of the bullet, resulting in a self-defense round that is engineered to perform. Poly...
$ 30.00
Lehigh Defense .38 Special +P Xtreme Penetrator Ammunition is the latest in their long line of premium personal defense and hunting rounds. Featuring a 100% solid copper construction and patent-pending CNC designed projectile. Xtreme Penetrators are among the best personal defense rounds on the market. This is loaded ammunition. Specs: Cal...
$ 27.95
OATH (Operators American Tactical Hardware) Tango Ammunition is developed to capture the expansion of a hollow point bullet while eliminating the possibility of feed malfunctions or material filling the hollow point cavity and preventing expansion. Oath's Tango bullet is a 100% copper two-staged projectile that features a "cap" which enables re...


text: Adrien, since you reported the bug, your feedback about the packages in -proposed is highly appreciated as well. Does it fix the issue for you, and does the package still work for you? -- getent group crashes winbindd on domain controller https://bugs.launchpad.net/bugs/328874 You received this bug notification because you are a member of Ubuntu Server Team, which is subscribed to samba in ubuntu.


text:
This wiki needs fun activities, although this wiki has to concentrate on the perfection of articles, I think users should be cut some slack, test your knowledge on the Flippin' Hard Adventure Time Quizzes - Week 1. There will be new quizzes each week.
- What keys did Wilberry Princess have to play in the episode: "Prisoners of Love"?
- In what episode did Flame Princess have her jewel missing from her forehead as an error?
- What is Pendleton Ward's favorite game on the Nintendo DS?
- Who designed the backgrounds for Adventure Time and when did he leave the show?
- What color were the two Woobeewoo that held up the chief Woobeewoo in "The Hard Easy"?
When you answer these questions, I will tell you how many you got correct, and how many you got wrong, but I won't tell you which ones were correct or wrong, the first person to answer them all correct will win a gif. A fancy gif, a gif so flippin' awesome they won't regret it.
So yeah. Have fun.


text: "Knack" marks a departure for Sony games' image, more famous for battle and adult action genres.Designers experimented with an over-sized remote controller to test how the buttons felt for tiny hands. "Knack" targets anyone from 7 to 70, according to Sony. The game can be played in easy or difficult mode, or a combination, allowing parents to play together with kids. "That was what made 'Knack' so appealing. It can be played by pretty much anyone on the easy setting," said Brian Ashcraft, senior contributing editor for New York-based Kotaku blog specializing in games. But Yusuke Watanabe, senior producer at the Japan Studio of Sony Computer Entertainment, the company's game unit, acknowledged one disadvantage for a character with thousands of parts. "It's going to be so hard making a mascot figure," he said with a laugh. __ Follow Yuri Kageyama on Twitter at twitter.com/yurikageyama


text:
Dismiss Notice
Welcome To CK5!
Registering is free and easy! Hope to see you on the forums soon.
Score a FREE Membership sticker when you sign up for a Premium Membership and choose the recurring plan.
1936-Present Suburban
The longest production run in automotive history deserves it's own forum.
Page 1 of 55
Thread Display Options
Page 1 of 55


text:
2016 Leadership Conference
Wednesday, January 13, 2016
The Second Annual Finding Your Path Leadership Conference is a one‐day event, showcasing inspirational figures sharing their unique journeys and insights that helped them to realize their individual successes.
Wednesday, January 13th, 2016
SECU Arena at Towson University
9:30am - 3:30pm
$5 parking available in lots 13 & 14
concessions open for lunch
Starting at only $20
Event Schedule:
Welcome - 9:30am
Matt Eversmann - 10am
Mia Hamm - 11:00am
Mark Butler - 11:45am
Lunch - 12:15pm
Marin Alsop - 1:30pm
Buck Showalter and John Harbaugh - 2:00pm
Closing - 2:45pm
*times are estimates and may vary slightly
Confirmed Speakers Include:
Manager, Baltimore Orioles
Head Coach, Baltimore Ravens
Women's Soccer
2 time World Champion & 2 time Olympic Gold Medalist
First Sergeant Matt Eversmann (Ret.)
decorated military leader who survived capture in Somalia and whose story is immortalized in the epic film, Black Hawk Down


text:
About iMo Controller:
iMo Controller is an early stage startup focussing on building innovative gaming products with the goal of changing the way you play computer games. iMo was co-founded in mid-2009 by Himanshu Baweja and Pankaj Meena. iMo was selected as Techcrunch50 finalist in 2009 and won the best presentation award. It's a long journey towards our goal and we are just getting started :).
A lot of people have worked together to make the iStrike possible. Without them you won't be playing iStrike today. They are (in no particular order):
- Saswat Kumar Sahu – the artist
- Sonaljit Mukherjee – the musician
- Kumar Sushant – the tester
- Gaurav Bhatia – the writer
- Pankaj Meena – the iPhone expert
- Tony Wang – the voice
- Debjeet Sen – the voice


text:
Craftsman Wallbed
Queen Size
Suggested Retail
Bed & Piers Sold Separately
Dimensions Closed / Open
* Queen Dimensions when closed: 66.5″W x 16.5″D x 75″H
* Queen Dimensions when open: 66.5″W x 98″D x 89.75″H
Wood Types
* Oak * Beech
Finish Options
* Red * Brown * Natural * Honey
* Light * Medium * Dark
Learn More About Our Craftsman Wallbed
The Craftsman Wall Bed is a perfect example of the Mission style furniture that was popularized in the late 19th century.
Our Craftsman wallbed is made out of quarter-sawn oak in your choice of finish. The stylish mission hardware gives it a traditional craftsman look.
There is also a wide selection of optional side cabinets and desks available that will complement your Craftsman murphy walled. Piers add storage for your guests while they stay or for you in your guest room or office.


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Difficulty uploading a file on our form under our upload fieldAsked by collectthemoney on July 25, 2016 at 08:13 AM
Our form has an option for uploading documents. Recently a few clients say that are unable to upload. However when we receive the completed from, the uploads were successfully loaded. The clients are saying that the website shows that the upload was not accepted. Other clients are not experiencing this problem.
One client sent us a copy of the error: under our "upload any necessary electronic files here" it said "0713125.pd 2.4MB Failed". Other clients are saying that the upload says "failed" also.
Any ideas on what is happening. Thanks for your reply.
JotForm Support
I have just checked your form, I have downloaded some of the submitted files that you received with the name "0713125" on it.
Here is a screenshot about my submission:
I can see that you provided this file name "0713125.pd", but I'm not sure if there is a missing "f" at the end, if the file name is correct then I think you should add this ".pd" extension in your form:
If the issue still persists, could you please provide us the details of those submissions?
We will wait for your response.
Hi Kevin,
Thank you for your reply. That was a typo on my part. The file extension that they tried to upload was a pdf.
So you did not have any trouble. But some of our clients are getting the previously stated error message. But on our end when we receive the completed form, we did received the uploaded documents. Sometimes the client tries 2 or 3 times and still is told it failed. Then on our end we end up receiving 2 or 3 submissions of the same documents.
Its confusing. Any thoughts?
Thanks for your help:)
JotForm Support
I have tried uploading two files to your JotForm using direct URL and it looks like the files are uploaded normally.
Is the form embedded on any of your web pages and experiencing the issue? Could you also check with your clients which browser they are using so that we'll try to replicate the issue at our end and provide you with necessary assistance.
Thank you!
Hi Kiran,
Thank you for your thoughts. I noticed that you tested files in KB. The file that I referenced above was 2.4 MB. Could size be a factor?
I will definitely check the browser issue. Our form is embedded on our web page, that is the only one that our client's use.
Thanks for your time:)
JotForm Support
I have just tested with some files with a size up to 2 MB:
As you can see they were uploaded without any problem, they were submitted without any problem as well.
Please do let us know if you were able to replicate the issue on another computer and provide us the details about the web browser used, so we can further test and try to replicate the issue.


text:
Slod - Walkthrough
By: Simon Melia
Welcome to the walkthrough for Slod on iOS and Android. The walkthrough contains the solution and will guide you through each of the levels with gameplay tips, tricks, hints and cheats, to enjoy Slod game on your iPhone, iPad and Android devices. Here, you'll have exclusive access to walkthroughs videos, level guides, missions, as well as a discussion section for each of the levels.
We provide a community for gamers to discuss, learn and share anything about Slod. Anyone can join in, share their experience and tips with others. If you have any questions, request or need extra help; feel free to ask by making a comment.
Slod Game Overview
Slod is available on iOS. For more details on the app, check out our review.
- Publisher: Simon Melia
- Developer: Simon Melia
- Genre: Puzzle
- Release Date: January 22, 2016
Gameplay & Videos: See it in Action
Want to get a high score in Slod? Don't miss any of the new videos of Slod gameplays played by pro gamers - Updates July 23, 2017.
Walkthrough Sections
Work in progress...
Note: Some of the levels still need walkthroughs. If you know how to beat a level, then please submit your level guide here and share your skills.Hit like if this helped you!
Stuck need help? Join the community
Ask questions about Slod and get help from other gamers.


text:
Beet Greens
Recipe by CJAY8248
From my collection of handwritten recipes 1960's.
Top Review by UmmBinat
This is perfect. DD1 (5 years old) also liked it quite a bit. It was my first time trying beet greens and I'm glad I chose this recipe. I used organic beet leaves off the beets that came in our weekly organic local produce basket, grey sea salt, a perfect salt, freshly ground black pepper, a little organic apple cider vinegar but not sure I would add vinegar another time, & organic local free range eggs (perfect tasting eggs where we have seen how they live on their farm). I'd like to make this again insha Allah (God willing).
See Discussion:3 Reviews
Ingredients Nutrition
- 3 lbs beet leaves (choose tender, young greens)
- 1⁄2 teaspoon salt
- 1⁄8 teaspoon pepper
- 2 tablespoons vinegar
- 2 tablespoons butter
- 2 hard-boiled eggs, sliced
Directions See How It's Made
- Wash the greens thoroughly several times in deep water. Cook in very little boiling salted water until just tender, a few minutes. Drain thoroughly. Season with salt , pepper, vinegar and butter. Garnish with sliced eggs over the top.


text:
Availability: Out of stock
was $60.50
Special Price $44.77
- Description
Thalgo Pure Freshness Tonic Lotion has now been discontinued and replaced with the Thalgo Eveil A La Mer Beautifying Tonic Lotion.
Used after the Pure Freshness Cleansing Milk and the Marine Foaming Cleanser, this lotion provides the skin with essential micro-nutrients leaving it clear, fresh and toned.
For women in search of cleansing products suited to their normal, combination or oily skin.
- Ingredients
IngredientsParaben Free
- Reviews
Write Your Own Review
- Application
ApplicationUse Morning and Evening. Apply Pure Freshness Cleansing Milk to the face and neck with gentle, circular movements. Remove with moistened cotton pads or tissue-off. Repeat for a thoroughly cleansed skin. Follow with Pure Freshness Tonic Lotion. Apply tonic lotion with cotton pads onto face and neck. Gently pat the skin dry if necessary.
- Shipping Info
Shipping Info
How long will my order take to be dispatched?
If the products in your order are showing as 'Ready for Dispatch' then they will be sent on the next available business day or same-day if you order before 12pm AEST. Products that are not in stock will generally be shipped within 2-5 business days.
How long will my order take to arrive?
Once dispatched, you will receive a tracking number via email. It usually takes 2-4 business days for express delivery and up to 7 days for standard delivery. Express next day delivery is only available for addresses in Australia Post's next day delivery network. New Zealand orders can take up to 10 business days due to customs clearance.
Is in-store pick up available?
Click & Collect for online orders is available in Western Australia. Our premise is located at Duncraig Shopping Centre in Perth, Western Australia. Orders are available for pickup on the next business day after the order is placed. You will receive a text message when your order is ready.
How much does it cost to have my order delivered?
If you spend over $89, delivery throughout Australia is FREE via Standard Delivery. For orders under $89, delivery starts at flat rate of $6.95. View the full table of rates below:
Orders Over $89 Orders Under $89 Standard Delivery (3-7 Business Days) FREE $6.95 Express Delivery (2-4 Business Days) $6.95 $11.95 Pick-Up Next Business Day (WA orders) FREE FREE International Delivery (New Zealand) $19.95
Protect Your Skin Against Pollution
Did you know, ground-level ozone...
Transform your skin in 2017 with Skinstitut
We all love setting goals and resolutions at the end of each year to...
Thalgo Cold Cream Marine for Dry &...
Thalgo has been at the forefront of marine skincare technology for...


text:
BELLEVILLE, Ill. — A St. Louis supermarket chain with a store in Bettendorf is facing a potential class-action lawsuit in Illinois related to a security breach affecting up to 2.4 million credit and debit cards of its customers.
St. Louis attorney Jeffrey Millar filed the lawsuit last week in St. Clair County. He filed it on behalf of a man who shopped at a Schnucks store in Belleville during the time the company says customers' accounts may have been compromised.
Maryland Heights, Mo.-based Schnucks Markets has said the breach dated to December and came to light in March. Many customers have reported fraudulent charges, some in the thousands of dollars.
Schnucks has posted a list of the affected stores and specific dates for each store at www.schnucks.com. The company reported that the Bettendorf store may have been affected between Dec. 10, 2012, and March 29.
The Belleville News-Democrat reports that Millar's lawsuit questions why the problem wasn't made public quicker.
A message left Thursday with Schnucks was not returned.


text:
No Tickets Left?
Provide Your Contact Information Below
05/26/14 9:00pm – The Wild Reeds: Brother Grand & The Herbert Bail Orchestra
See All Brother Grand Events
Brick & Mortar Music Hall
May 26, 2014, Monday @ 9:00pm
With millions of fans nationwide, Ticket Monster
is your destination for exclusive discounts on
sports, concerts, and theater tickets.
Sign up and never miss a deal!
Enter your email address and we will send you an email with instructions on how to reset your password.


text:
国内特許検索 >
PROBLEM TO BE SOLVED: To provide high-performance activated carbon using cocoons, capable of being worked in a plate shape, a cylinder shape, a rod shape or another arbitrary shape according to the usage pattern of activated carbon, and having a large specific surface area.
SOLUTION: The high-performance activated carbon is made of a carbonized material obtained by carbonizing cocoons and has a specific surface area of >3,000 m2/g. A method for producing such high-performance activated carbon is also provided which includes a low-temperature carbonization step of carbonizing cocoons by heating to a predetermined temperature in a low temperature region of 300-400°C, and an activation/high-temperature carbonization step of carbonizing the cocoons carbonized at the low-temperature carbonization step by heating to a predetermined temperature in a high temperature region of 750-850°C after infiltrating a basic aqueous solution into at least surfaces of the cocoons.


text:
Almost a year since the onset of the stock market crisis, investors and brokers have learned some valuable lessons. Wall-Street has interviewed stock market specialists and compiled a list of top 10 mistakes to which investors can fall victim in post-recession planning.
In lipsa unui acord scris din partea InternetCorp, puteti prelua maxim 500 de caractere din acest articol daca precizati sursa si daca inserati vizibil linkul articolului 10 important don'ts in post-recession planning.


text:
You are a fan of economies dong ? Come to discover the best forums economies dong on the internet and share your experience with a community fan of economies dong . You can also build a forum economies dong and create your online discussion.
A Dinar Investment Reality Community
neno, nenos, place, iraq, dinar, baghdad, kurdistain, vietnam, #dong, revalue, reinstated, domination's, lower, denoms, treasury, vault, sterling, currency, community, kuwait


text:
Broke Down BitchesFavorites [+]
Pay-Per-Minute Viewing
You can now enjoy this movie on your iPhone®, iPad®, or compatible Smart TV & Android devices.
Purchase Options
Pay-Per-MinutePurchase Minutes
Streaming Rentals
Available MP4 sizes:SD 480p (2.18 GB)
DRM-free downloads offer content that is not limited by standard Digital Rights Management (DRM) access technologies. These technologies allow hardware manufacturers, publishers, copyright holders and individuals to limit the usage of digital content.
When you purchase DRM-free downloadable content, that content is available for you to download on any computer and save to a disk whenever you like. You can also download to multiple computers. There is no timeframe associated with your purchase and the content will not expire.
Running Time: 140 minutes
Released: 04/2009
Studio Name: Acid Rain Grind House Porn
Series: Broke Down Bitches
Director: Jax
Categories: Natural Breasts Gonzo Reality Based
Description: We were driving down the highway when we saw this total hottie waving us down. Can you believe this dumb broad ran out of gas? It was all good because first we filled up her tank, and then we filled up her tight little box. The gas was $3.20 a gallon, but the cum was free!
5 Scenes from Broke Down Bitches
Watch with a Subscription to the SuperPass Channel, with Pay-Per-Minute or Streaming Rental
Broke Down Bitches: Scene 1 Free Preview
Starring: Barrett Blade Ryder Skye ... More Details
Stars: Barrett Blade, Ryder Skye
Sex Acts: Vaginal Sex, Oral Sex, Choking
Positions: Side Saddle, Reverse Cowgirl, Sitting, Standing
Broke Down Bitches: Scene 2 Free Preview
Starring: Evie Delatosso Jay Huntington ... More Details
Stars: Evie Delatosso, Jay Huntington
Sex Acts: Breast Play, Vaginal Sex, Oral Sex
Positions: Doggy Style, Cowgirl, Piledriver, Reverse Cowgirl, Side Saddle, Standing Doggy Style, Standing
Broke Down Bitches: Scene 3 Free Preview
Starring: Nadia Nitro T.J. Cummings ... More Details
Stars: Nadia Nitro, T.J. Cummings
Sex Acts: Vaginal Sex, Oral Sex
Positions: Cowgirl, Standing Missionary, Reverse Cowgirl, Side Saddle, Laying
Settings: Bedroom
Broke Down Bitches: Scene 4 Free Preview
Starring: Leilani Li James Deen ... More Details
Stars: Leilani Li, James Deen
Sex Acts: Vaginal Sex, Oral Sex
Positions: Side Saddle, Doggy Style, Laying, Kneeling
Broke Down Bitches: Scene 5 Free Preview
Starring: T.J. Cummings Rucca Page ... More Details
Stars: T.J. Cummings, Rucca Page
Sex Acts: Breast Play, Vaginal Sex, Oral Sex
Positions: Cowgirl, Reverse Cowgirl, Kneeling, Sitting, Side Saddle, Laying
Recommended Movies
AEBN Cams


text:
Funkiest Shoes Designed by Celebrities
Feb. 28, 2009 No Comments Posted under: Footwear
Shoes come in all shapes, size and colors, but by and large, many of the shoes we wear go unnoticed. If you are the type who likes to stand out in a crowd, or loves things that are different, these designers,celebrities and makers of funky shoes will more than likely appeal to your unique sensibilities. here are 10 funkiest designs by celebrities.


text:
Monday, May 13, 2013
FREE Blogger Opportunity - Kiss the Cook Giveaway
A product sample was not received for review. I was not paid in any form of cash for this posting. The opinions expressed are my own and were NOT influenced in any way.
Organized by: Mom Powered Media
Prize: Rachael Ray Cookware Prize Package
Event dates: 6/3 - 6/24
Bonny Sallee
Posted by Bonny Sallee at 5/13/2013 11:38:00 PM
Labels: Blogger Opportunity
Subscribe to: Post Comments (Atom)


text:
Your cart is empty.
Snack Rewards
earned on this order
Candies, Food & Snacks
Nutrition Bars
Energy Bars
Tiger's Milk Bars
Item # BFG 30304 by Tiger's Milk Bars (Mfg. Part # 30304, UPC # 074345-103726)
|Receive Betty's Coupons, Special Offers & Promotions!
- My Account
- Company
- Promotions
- Shopping Guides
Unless you are a licensed care provider or similar
qualified entity, a prescription is required
to purchase this item.
Thanks! -- Betty Mills


text:
2 year old filly by Brookstone Bay out of Silver Sparkles by strawfly special/beduino. This filly is currently in race training. Sound and sane. one…SOLD
2013 filly by AAA son of pyc paint your wagon, BURNIN DIESEL SI 98 out of bailey sue dash by dashin is easy. dams first foal is a yearling this year.…SOLD
2010 gelding by multiple graded stakes winner Frankie Shoots out of Pie Opener SI 85 by Mr Eye Opener. This gelding has been run a few times at the brush…SOLD
4 year old gelding by AAA son of corona cartel, exclusive corona, out of downtown daytona by mr eye opener. this gelding is in race training, SI 85 6…SOLD
2011 Filly by Power Storm (tb) out of AAA producer Ryendas Wind by Windy Ryon. half sister to Mr Bisiesto SI 93 LTE $20,000. Currently in race training…SOLD
2011 gelding by stakes producer captain courage (mr jess perry - corona chick) out of hy diamond sweetie by moon lark. currently in race training. sound…SOLD
2013 filly by CHAMPION PRODUCER STOLI! out of stakes winner EL STREAKER (packin sixes - slammin bammie) lte $146,201. This filly comes from a BLACKTYPE…SOLD
2007 stallion by champion producer stoli out of aaa producer dazzles royal dash by royal quick dash. He comes from the same family as Teller cartel! 3rd…SOLD
Bayard, NE


text:
Natalie Allen who's a news anchor was created on 11th August of the year 1962. She's the native of Memphis, Tennessee, United States of America. She's among the well-known faces in the American news business. After that, she attended the Graduate Study Political Science in the University of Memphis.
At the start of her broadcasting career, she began working at the KFSM TV situated in Fort Smith, Arkansas. She subsequently moved to the WREG TV which is situated in Memphis where she worked as reporter along with an anchorman for few years. While there, she managed to bag Regional Emmy Award along with the Edward R. Murrow Award in procedure. Along with that, Allen was also an anchorman in the CNN from 1992 till 2001. She'd also made her appearance on The Nightly News, The Today Show, along with CNBC as correspondent for the NBC.
Along with that, she'd also covered 2004 Presidential Inauguration for the MSNBC at the same time as she in addition has reported live on funeral for the President Ronald Reagan in the Washington. Prior to that, she was likewise the full time environment along with the climate correspondent including a news caster through the year 2007 till 2009 for: The Weather Channel. She's the first person to be recruited as anchor and correspondent with no weather as the meteorology expertise along with history.
To discuss her private life biography, there's gossip that Natalie hasn't got married yet nor has any husband. However, the gossip is not verified. She's maintained to help keep her profile quite low so there's no any more advice found related to her private life biography. Natalie was also the top ranking man in the CNN's major channel who had been put in Super Hair Ultimate 50 list.
Besides that, Natalie has reached in the age of 52. She stands taller in the height of five feet and eight inches. Although she's now in her early fifties, she seems really amazing, enchanting along with hot. She's got an appealing and perfect body measurement of 34-26-35. She's got slim set of legs which she flaunts while wearing the formal dresses. Besides that, she can likewise be followed in different social media including Twitter, Facebook and Instagram account.


text:
ACU encourages staff and students to cycle to campus.
There are a range of cycle paths near campus, see Brisbane City Council Cycling for further details.
There are two sets of bike racks located on campus. The first set is located outside of the Library adjacent to the covered seating area. The second set is located just outside the Co-Op Bookshop.


text: To get from Ruvo di Puglia to Treviso by train you can catch one of the 29 trains that everyday travel between these two cities. The first train to Treviso leaves from Ruvo di Puglia at 03:25 and the last train departs at 23:27. The trip by train from Ruvo di Puglia to Treviso last on average 06:13 travelling with high-speed trains (Frecciarossa or Italotreno) If you choose a local train the trip lasts on average 06:16 hours. For your trip from Ruvo di Puglia to Treviso by train you can choose to leave from one of Ruvo di Puglia main stations: Salerno, Molfetta On Wanderio you can compare all schedules, fares and railway companies to Treviso and book the best itinerary starting from Ruvo di Puglia.


text:
Thankstruthing is the time to bring truth into the fourth Thursday of November every year. It originated shortly after the holiday invented by Pilgrims known as Thanksgiving, where people indulge in the sinful acts of gluttony and sloth. The Pilgrims were resting lazily after the tryptophan from their turkey kicked in and realized that nothing truthful happened in their harvest. They decided to rename Thanksgiving Thankstruthing after a divine intervention by Dr. Stephen T. Colbert, DFA.
CelebrationEditThankstruthing is celebrated in many ways. The most traditional celebration involves the good ol' fashioned hand turkey. But the Thankstruthing hand turkey has a twist: It's actually a Nancy Pelosi hand turkey.
- Get a blank piece of paper
- Slam your fist down in frustration (no crying)
- Trace hand
- Add hair
- Add frowny face
Make many of these Nancy Pelosi hand turkeys and hang them on the refridgerator, walls, and doors to remind yourself how your vote for a democratic house destroyed America.
Other methods of celebration include the making of truthsicles, violent hunts for Thankstruthing turkeys, truth-egg hunts, and playing truthball.
Similar to Thanksgiving, Thankstruthing is chock-full of food to stuff your face with.
- Turkey (with the liberal cut out... usually in the dark meat)
- Stuffing (truth-flavored)
- Truth Potatoes
- Truth-on-the-cob
- Green Bean Cassatruth
- Bread Rolls of Truth
- Kraft Crumbles (epitome of American Food)
- Truthberry sauce
- Pumpkin Pie (Made from truth-pumpkins)
People who Celebrate ThankstruthingEdit
While it is to be celebrated by every America-loving citizen, the following people celebrate Thankstruthing better than everybody else:
- Stephen Colbert
- The Greatest President Ever
- Aunt Jemima
- Christians
- George L.
- Ted Haggard
- Jesus H. Christ


text:
Here is your weekly ad and coupon matchups for the Whole Foods Market South Weekly Ad that runs from 12/16-12/22.
You can view the full Whole Foods coupon policy here. Here are a few highlights: you can combine a store and manufacturer's coupon on the same item. Multiple store coupons are allowed when you buy identical items as long as you have a physical coupon for each one you purchase.Coupons are not doubled or tripled and no overage is given when the coupon value exceeds the price of an item. Case discounts can be combined with coupons.
Allegro Coffee Celebration Holiday Coffees – $4.99
Includes: Select varieties
Traditional Medicinals Tea – $3.99
Includes: Select varieties
- $1.00/1 Traditional Medicinals Tea – Whole Foods 2015 Calendar (exp. 12/31/15)
Final Price: $2.99
Natural & Organic
Tomatoes, Beefsteak, Organic, per lb – $2.99
Out-of-Pocket Price: $2.99
Final Price: $2.74
Buy 1 pound. Pay $2.99 OOP. Submit for $0.25 rebate. Final price: $2.74
Pears, Comice, per lb – $0.99
Never miss another hot coupon, deal or freebie again! "Like" Couponaholic .NET on Facebook, Follow us on Pinterest, Follow us on Twitter, Follow us on Instagram or Subscribe to our Daily Email Newsletter!


text:
Join Date: Aug 2006
Posts: 2
August 29th, 2006, 1:08 AM #1
Timing chain or timing belt?
Hey, which does the 2.4 SS sedan have, a timing belt or a timing chain?
Join Date: Nov 2006
Posts: 638
December 18th, 2006, 2:48 PM #2
RE: Timing chain or timing belt?
ALLLLL GM are chains, only imports are belts


text:
If an item you ordered from TextbookRush does not meet your expectations due to an error on our part, simply fill out a return request and then return it by mail within 30 days of ordering it for a full refund of item cost.
Learn more about our returns policy
Description: This key reference is a primer on energy in a state that continues to lead the world in finding sustainable solutions to one of the most pressing issues of the twenty-first century. While much public debate has focused on fossil fuels, this clearly written guide provides essential information on a broader range of issues--where our energy comes from, where future supplies will be found, and what new advances are being made in the area of renewable energy sources. Making the complex world of energy science and policy accessible to a wide audience, Peter Asmus examines the rich human history of California's earliest oil and hydroelectricity developments, explains the natural history underpinning the state's cornucopia of energy sources, covers such controversial sources as nuclear reactors and liquified natural gas, and more. Introduction to Energy in Californiaincludes: * Discussion of oil, nuclear power, coal, emerging alternative technologies, and renewable sources including geothermal, solar, wind, and hydropower * Analysis of the challenges and solutions facing California and the world on energy-related issues such as global climate change * Compelling case studies of corporations, governments, communities, and individuals working on today's most pressing energy questions * Color illustrations, useful maps, and clear graphics throughout
Limited time offer:
Get the first one free! (?)
All the information you need in one place! Each Study Brief is a summary of one specific subject; facts, figures, and explanations to help you learn faster.
List price: $24.95Copyright year: 2009Publisher: University of California PressPublication date: 7/6/2009Binding: PaperbackPages: 376Size: 4.50" wide x 7.50" long x 1.25" tall Weight: 1.342Language: English
Peter Asmus, President of Pathfinder Communications, is a journalist, consultant, and author of Reaping the Wind: how Mechanical Wizards and Profiteers Helped Shape Our Energy Future, among other books. Art Rosenfeld is California Energy Commissioner and Professor Emeritus of Physics at the University of California, Berkeley. Energy journalist Arthur O'Donnell is executive director of the Center for Resource Solutions.
*A minimum purchase of $35 is required. Shipping is provided via FedEx SmartPost®
and FedEx Express Saver®. Average delivery time is 1 – 5 business days, but
is not guaranteed in that timeframe. Also allow 1 - 2 days for processing. Free shipping is eligible only in the continental United States and excludes
Hawaii, Alaska and Puerto Rico. FedEx service marks used by permission."Marketplace"
orders are not eligible for free or discounted shipping.
Learn more about the TextbookRush


text:
The tammar wallaby major histocompatibility complex shows evidence of past genomic instability
The major histocompatibility complex (MHC) is a group of genes with a variety of roles in the innate and adaptive immune responses. MHC genes form a genetically linked cluster in eutherian mammals, an organization that is thought to confer functional and evolutionary advantages to the immune system. The tammar wallaby
Here we describe the 'core' tammar wallaby MHC region on chromosome 2q by ordering and sequencing 33 BAC clones, covering over 4.5 MB and containing 129 genes. When compared to the MHC region of the South American opossum, eutherian mammals and non-mammals, the wallaby MHC has a novel gene organization. The wallaby has undergone an expansion of MHC class II genes, which are separated into two clusters by the class III genes. The antigen processing genes have undergone duplication, resulting in two copies of TAP1 and three copies of TAP2. Notably, Kangaroo Endogenous Retroviral Elements are present within the region and may have contributed to the genomic instability.
The wallaby MHC has been extensively remodeled since the American and Australian marsupials last shared a common ancestor. The instability is characterized by the movement of antigen presenting genes away from the core MHC, most likely via the presence and activity of retroviral elements. We propose that the movement of class II genes away from the ancestral class II region has allowed this gene family to expand and diversify in the wallaby. The duplication of TAP genes in the wallaby MHC makes this species a unique model organism for studying the relationship between MHC gene organization and function.
The major histocompatibility complex (MHC) is a group of immune genes critical for immune response to pathogens, immunoregulation, anti-tumour responses and inflammation. Disease resistance and susceptibility associations have been identified between MHC genes and autoimmune diseases
The MHC of eutherian mammals is a large cluster of linked genes, broadly divided into three regions, class I, class II and class III. These regions are named for the primary type of MHC gene found within them. The class I and class II MHC genes encode molecules responsible for antigen presentation. The class I region contains class I genes, which present endogenous peptides to CD8+ T cells, and also contains a collection of well conserved genes with varying functions known as the framework genes, including the members of the TRIM family of genes, FLOT1, TUBB and NRM
The human MHC spans 3.6 Mb and includes 264 genes
Among non-mammals a diversity of MHC 'shapes and sizes' has been identified. For example, the MHC region of the chicken (the B locus) is considered to be 'minimal essential' spanning 92 Kb and containing only 19 genes
Characterizing the MHC of distant mammals will provide insights into how the MHC evolved in vertebrates. Marsupials and eutherians last shared a common ancestor approximately 148 million years ago, and since then their immune systems have been evolving independently under different pathogenic pressures, making marsupials ideal for comparative studies of the MHC region. We previously annotated the MHC of the grey short-tailed opossum (
Comparison of the opossum MHC with that of another marsupial species is important as marsupials are an evolutionarily diverse group with orders in both South America and Australia. The tammar wallaby (
MHC gene organization in the wallaby
In total 4.7 Mb of tammar wallaby MHC sequence containing predictions for 129 putative functional genes has been generated from chromosome 2q. The BACs assemble into nine contigs plus three orphaned BACs (summarized in Table
Table 1
Summary of contigs across chromosome 2q
No. of coding genes
Figure 1
Diagram of the organization of the wallaby MHC with gene annotation
Diagram of the organization of the wallaby MHC with gene annotation. Colour code for genes; yellow - extended class II, blue - class II, red - class I, purple - antigen processing genes, pink - olfactory receptors, grey - pseudogenes. The overlapping BACs are indicated by black lines below the annotation. BACs not assembled into a contig are indicated by the BAC name. The KERV fragments are indicated by thick black arrows. An OSCAR fragment is indicated with an arrow.
Figure 2
Metaphase and interphase FISH showing the location of anchored BACs in order to order the contigs
Metaphase and interphase FISH showing the location of anchored BACs in order to order the contigs. (a-d) FISH on tammar walaby interphase nuclei showing (a) co-localization of contig 2 (BAC_241L16 in red) and contig 1 (BAC_288B16 in green), b) the positions of contig 2 (BAC_241L16 in red) and contig 6 (BAC_310P15 in green) relative to one another, c) the position of BAC_212C16 relative to contig 1 (BAC_288B16) and contig 6 (BAC_310P15), BAC_212C16 is midway between the two contigs, d) the position of BAC_210A8 relative to contig 1 (BAC_288B16) and contig 6 (BAC_310P15), BAC_210A8 is closer to BAC_288B16 than BAC_310P15. e) Metaphase FISH showing that contig 4 (BAC_244N6 in red) is telomeric to contig 6 (BAC_310P15 in green), f) Metaphase FISH showing that BAC_171E14 in red is approximately 1 Mb telomeric to contig 6 (BAC_310P15 in green).
Contig 1 covers a 711 Kb region and includes the extended class II region, class II DM α and β genes, a single class I gene, several antigen processing (TAP1 and TAP2) and proteasome genes (PSMB8 and PSMB9) (Figure
Figure 3
Schematic comparison of the opossum MHC class I/II region and the putative class I/II region in the tammar wallaby
Schematic comparison of the opossum MHC class I/II region and the putative class I/II region in the tammar wallaby. Colour code for genes; blue-class II, red-class I, purple-antigen processing genes, grey-pseudogenes. Each 100 Kb is represented by a short black line.
Contig 2 covers a 784 kb region and contains class I and antigen processing genes. It does not overlap Contig 1 but based on interphase FISH results it lies adjacent (Figure
In the opossum a class II DBB gene (
Contig 3, a minicontig of ~300 kb, contains a class II DAB processed pseudogene and a cluster of olfactory receptor genes and has been mapped by FISH to the region between Contig 2 and Contig 4 (Figure
Contigs 4 and 5 contain 44 class III genes, class II genes belonging to the DC gene family and a cluster of butyrophilin (BTN) genes. The gene content and order of the 44 class III genes is nearly identical to that of the opossum. Comparison of Contigs 4 and 5 with the opossum class III region suggests that the contigs are separated by ~150 kb and we predict that nine class III genes found in the opossum fall into this gap in the wallaby MHC. Similarly, Contigs 5 and 6, containing framework genes, are separated by ~250 kb based on comparison with the homologous opossum region.
Contigs 7, 8 and 9 contain a cluster of class II genes belonging to the DAB gene family interspersed with DAB pseudogene fragments. These contigs map (by FISH) to a region ~1 Mb telomeric of the framework region (Figure
Wallaby TAP Genes
Within the wallaby MHC class I region there has been an expansion of the antigen processing genes, TAP1, TAP2 and PSMB. The TAP1 genes with complete open reading frames are found on BAC 242G6 (TAP1A-Contig 1) and BAC 6E22 (TAP1B-Contig 2), while the complete TAP2 genes are found on BAC 242G6 (TAP2A), BAC 7D13 (TAP2C) and BAC 146G20 (TAP2B) (Figure
Figure 4
Alignment of TAP1 genes from the wallaby and expressed transcripts isolated from spleen, blood and EST library
Alignment of TAP1 genes from the wallaby and expressed transcripts isolated from spleen, blood and EST library. Amino acid alignment of TAP1 sequences. TAP1A_242G6 and TAP1B_6E22 are sourced from BACs in Contigs 1 and 2 respectively. MaeuTAP1A_Animal1 was sourced from a the spleen of animal1, TAP1B_EST is sourced from a mixed tissue EST library. MaeuTAP1A_Animal1, MaeuTAP1A_Animal2 and MaeuTAP1A_Animal3 were sourced from blood samples from Animals 1, 2 and 3 respectively. HosaTAP1 is included for comparison. Dashes indicate missing sequence, while dots indicate conserved residues. Exon boundaries are indicated by a number above the first residue of the exon. Residues thought to interact with the peptide are indicated by a line above the sequence.
Figure 5
Alignment of TAP2 genes from the wallaby and expressed transcripts isolated from spleen, blood and EST library
Alignment of TAP2 genes from the wallaby and expressed transcripts isolated from spleen, blood and EST library. Amino acid alignment of TAP2 sequences. TAP2A_242G6, TAP2B_146G20 and TAP2C_6E22 were sourced from BACs in Contigs 1 and 2. MaeuTAP2A_EST and MaeuTAP2B_EST were sourced from a mixed tissue EST library. MaeuTAP2A from Animals 1, 2 and 3 were sourced from blood samples. HosaTAP2 is included for comparison. Dashes indicate missing sequence, while dots indicate conserved residues. Exon boundaries are indicated by a number above the first residue of the exon. Residues thought to interact with the peptide are indicated by a line above the sequence.
Figure 6
Neighbour-joining phylogenetic tree showing the relationship between TAP1 and TAP2 genes from the wallaby and other vertebrates
Neighbour-joining phylogenetic tree showing the relationship between TAP1 and TAP2 genes from the wallaby and other vertebrates. Analysis was performed on the full amino acid sequence for each gene.
The TAP promoters were examined by aligning the 200 base pairs of sequence upstream from the putative transcriptional start sites with the promoter sequences from human and opossum TAP genes. The TAP1A and TAP1B genes share 70% nucleotide identity across this region, emphasizing the distinctiveness of these genes from one another. The TAP1A and TAP1B genes share 47 and 50% identity respectively with the same regions of the human TAP1 gene. A potential ISRE, NF-kB site and GC sites were identified for both TAP1B and TAP1A. The TAP2A and TAP2B genes share 80% nucleotide identity across the promoter region and 50-55% identity with the homologous region of TAP2C (data not shown).
To determine whether all of the TAP1 and TAP2 genes are expressed, primers were designed to amplify exons 5 and 6 as the sequences of the two paralogs differ in this region (summarized in Table
Table 2
TAP variants found in spleen, blood or combined tissues from four different animals.
Animal 1
Animal 2
Animal 3
Animal 4
Combined tissue ESTs
Rearrangement of the MHC region
A KERV (Kangaroo Endogenous Retrovirus) fragment was identified next to the non-MHC pseudogenes at position 569 kb of Contig 2 on BAC93J23. KERV fragments were also identified adjacent to the class III region and the VAMP4 pseudogene on BAC198J4 and BAC163H18 and next to the class II DAB cluster on BAC 178C11 and the DBB genes on BAC 171E14.
The class II antigen presenting genes have duplicated and form two clusters separated by the class III genes
MHC class II genes are found on nine of the sequenced BACs (Figure
The classical class II genes are found in two regions: the first lies between the antigen processing genes and the class III genes, and contains the DBA, DAA and DBB genes. The second is found at the telomeric end of the region and contains DAB genes as well as additional DBB and DBA genes.
There is a minimum of 7 and a maximum of 10 DAB loci, however, heterozygosity in the individual from which the BAC library was made and the complexity and polymorphism of this gene family means the number of DAB loci is difficult to resolve. Five DAB genes are found on Contig 7. Four are found on Contigs 8 and 9. A further DAB gene is located on BAC 285B7. All of these DAB genes have complete open reading frames and contain residues consistent with functional genes. As many of the DAB sequences are closely related (share between 83 and 92% nucleotide identity across the entire coding region of the gene) and are interspersed with pseudogenes and DAB gene fragments it is difficult to determine if some identified DAB genes are representative of different haplotypes. For example, we predict that Contigs 7 and 8 could represent different haplotypes as 244N6.5 (Contig 7), 243M2.1 (Contig 8), 178C11.2 (Contig 7) and 155M2.3 (Contig 8) are highly similar DAB genes. Contig 9 is more difficult to place and may represent additional loci or alleles. This suggests that wallabies have 6-8 DAB loci. Comparison with known wallaby DAB transcripts indicates that at least five of these genes are expressed (Figure
Figure 7
Neighbour joining phylogenetic tree showing the relationship between mammalian class II B chain genes
Neighbour joining phylogenetic tree showing the relationship between mammalian class II B chain genes. Analysis was performed on the amino acid sequence from the β2 domain only.
The DBB, DBA and DAA genes have been physically mapped to two different locations separated by the class III region. BAC_171E14 containing two DBB genes and one DBA gene lies adjacent to the DAB gene cluster telomeric to the MHC. A second BAC (210A8), containing an additional DBB gene, a DBA gene and a DAA gene lies between the class I/antigen processing genes (Contig 2) and the class III region. No intact class II genes are found on Contig 2. The corresponding region to Contig 2 in the opossum contains DBB, DBA and DAA genes located adjacent to the class I genes,
We predict the wallaby has four DBB genes, one DAA gene and two DBA genes. The wallaby DBB genes share high levels of sequence similarity, between 92 and 94% nucleotide identity across the entire coding region, and 83 and 88% nucleotide identity across the peptide binding region. Phylogentic analysis shows that the DBB genes form a species specific clade and
The wallaby MHC has undergone extensive rearrangement since the divergence of the Australian and American marsupials. The classical class I genes have moved out of the core MHC region on chromosome 2q and are found at 10 separate chromosomal locations
The classical class I and antigen processing genes are not linked in the wallaby
Linkage of classical class I and antigen processing genes within the MHC has been shown to have functional significance in vertebrates. This is most pronounced in non-mammals
We have previously shown that classical class I genes are not found within the core MHC on wallaby chromosome 2q
Most vertebrate species have only a single TAP1 and TAP2 gene, which form a heterodimer on the ER membrane. In mammals the TAP molecules are generally permissive in the peptides they pump into the ER and it is the class I molecules that are selective in the peptide they will bind. The wallaby has multiple TAP1 and TAP2 genes. It appears that TAP2B (BAC 146G20) and TAP1B (BAC 6E22) arose via duplication events from the TAP1A and TAP2A genes on 242G6 (Figure
Genomic organization of class II
In some non-mammals, class II genes are located in a single cluster next to the class I genes. Similarly, the opossum, which last shared a common ancestor with the wallaby ~80 mya contains a class I/II region
Class II copy number
The class II genes have undergone large scale expansion and rearrangement since the divergence of the American and Australian marsupials. The tammar wallaby is the only marsupial species for which the number of DAB genes has been determined using large scale sequencing, as the DAB genes were not sequenced in the opossum genome, and only a single gene has been characterized at the cDNA level
Only a single DAA gene was identified 1 Mb away from the cluster of DAB genes and two DBA genes were identified. Similarly, there is evidence for a single DAA gene and multiple DBA genes in the brushtail possum
Class II heterodimers
Based on the organization of the class II genes in the wallaby we predict that the DAB and DAA genes form heterodimers, while the DBB and DBA genes most likely form heterodimers. Holland and colleagues (2008) recently proposed that in brushtail possums, highly variable DAB genes form heterodimers with the almost monomorphic DAA genes and the somewhat polymorphic DBB genes form heterodimers with the DBA genes
Kangaroo endogenous retrovirus and gene rearrangements
Kangaroo endogenous retrovirus (KERV) was originally discovered due to its role in macropod chromosome rearrangement and evolution
In a broader context, analysis of retroposon insertions within the South American and Australian marsupial orders has shown that the Australian marsupials derived from a single common ancestor, indicating a single marsupial migration from South America to Australia
The wallaby MHC has undergone extensive rearrangement since this species shared a common ancestor with the South American marsupials. Although the remnants of a class I/II region, seen in the opossum and non-mammals, are visible in the wallaby there are no classical class I genes within the MHC. This is remarkable for a mammalian MHC and may have affected the number of antigen processing genes and their expression, resulting in multiple combinations of TAP heterodimers. The movement of class I and class II genes may have facilitated the generation of diversity within these gene families. The wallaby class II genes are found in two regions separated by the class III genes and this most likely triggered the expansion of the highly polymorphic class II DAB family of genes. Analysis of the wallaby MHC has provided insights into the evolution of this gene family in marsupials and shed light on factors that have influenced the evolution of the MHC in this branch of mammals.
Selection of MHC-associated BAC clones
Forty-nine overgo probes were designed based on annotated opossum MHC genes (Additional File
Additional file 1
Table S1. Overgo probes used for BAC isolation.
Click here for file
Overgo probes were radio labeled with 32P-dATP and 32P-dCTP (GE Healthcare) and used to screen a 11 × tammar wallaby BAC library (Me_KBa, Arizona Genomics Institute, USA) using the BACPAC hybridization protocol (available at:
Secondary Screening of MHC associated BAC clones
Secondary screening of positive BAC clones was used to determine BACs positive for individual MHC genes and to remove false positive clones. BAC clones were cultured overnight and 1 ul of culture was applied to gridded Hybond N+ membrane (GE Healthcare). The membranes were placed on LB/agar plates with chloramphenicol (12 μg/ml) and incubated at 37°C overnight. The membranes were removed from the plate and placed on blotting paper moistened with a denaturing solution for 7 min, followed by blotting paper moistened with a neutralizing solution for 7 min. The membranes were rinsed in 2 × SSC and baked for 2 hours at 80°C. Ten overgo probes (Additional File
Physical Mapping of BACs
Many MHC class I genes of the tammar wallaby are known to be located outside the MHC. Thus, BACs known to contain class II MHC genes were physically mapped using Fluorescent In Situ Hybridization (FISH) according to Deakin et al. (2007)
Interphase nuclei preparations
A male tammar wallaby fibroblast culture was grown to confluency and held without medium change for 3 days to enrich for G1 interphase cells. Cells were harvested by trypsinisation, washed twice in PBS, swollen in 75 mM KCl at 37°C for 15 min, fixed in 3:1 methanol: acetic acid and dropped onto glass slides. Three separate experiments were performed on interphase nuclei for each orphaned BAC. BAC 310P15 representing the framework region/Class III contig and 288B16 representing the extended region were directly labelled by nick translation with SpectrumGreen (Vysis) and the orphaned BAC was labelled with SpectrumOrange (Vysis). In experiment one all three BACs hybridised to nuclei in the same experiment elucidated whether orphaned BACs were within the MHC spanning from the Class III region to the extended Class I/II region. Two further experiments were carried out with either BAC 310P15 or 288B16 labelled with SpectrumGreen and the orphaned BAC labelled with SpectrumOrange allowed the orientation of the orphaned BAC in relation to the flanking region BACs to be ascertained. Hybridisation of labelled probes to interphase nuclei was carried out following the FISH hybridisation protocol described in Deakin et al (2008). A total of 50 nuclei were imaged for each interphase experiment.
BAC contig assembly
All BAC clones were assembled into contigs using BAC fingerprinting as described by Marra
Sequencing of overlapping BACs
Sequencing of BACs occurred at the Wellcome Trust Sanger Institute as previously described
Phylogenetic and sequence analysis
The overlapping regions of fully sequenced BACs were determined using Sequencher 4.1.4 (GeneCodes) with 10% minimum overlap and 80% minimum nucleotide identity. The overlapping regions were then checked manually for mismatches. The predicted, full length coding sequences of the MHC class II α chains and β chain and TAP genes were aligned with the sequences from the NCBI database listed below using ClustalW, in the Bioedit program
TAP sequences used for phylogenetic analysis were as follows: Opossum: ModoTAP2A, ModoTAP2B and ModoTAP1 can be found at
MHC class II β chains sequences used for phylogenetic analysis were as follows: Brushtail possum: TrvuDAB,
Analysis of TAP1 and TAP2 expression
Primers were designed to amplify exons 5 and 6 of the wallaby TAP1A, TAP1B, TAP2A, TAP2B and TAP2C genes (TAP1F-CTGTGGAGGCACTTTCTGC, TAP1R-. CATCGGTCACCATCTTTCC, TAP2F-TTGGAGCAGAGGAGGATGA, TAP2R-GAGTAGGAATGAGACAAGGC). The primers were designed to regions where the multiple TAP1 and TAP2 genes are identical, but across a region that would allow different genes to be identified. TAP1 and TAP2 fragments were amplified from a spleen sample and blood samples (n = 3) with the following reaction: 1 × Buffer, 2 mm MgCl2, 200 μm dNTP, 2 μm of each primer and 0.3ul of High fidelity taq polymerase (Expand taq, Roche). Cycling conditions were as follows: Initial denaturation at 94.0°C for 3 min, followed by 29 cycles of 94.0°C for 30 s, 57°C for 30 s, and 72°C for 40 s, and a final extension at 72°C for 10 min. A 250 base pair fragment was amplified and cloned into a commercial vector (Clonejet, Fermentas). Twelve clones were selected from each spleen or blood sample and sequenced using a M13F and M13R primers. A 5' and 3' EST database constructed from mixed tissue of a single wallaby (including spleen and lymph node) was blasted using full length TAP1A, TAP1B, TAP2A, 2B and 2C sequences. Access to the library was kindly provided by Marilyn Renfree at the ARC Centre of Excellence in Kangaroo Genomics.
Authors' contributions
KB and SB designed the project. HVS isolated BACs, carried out phylogenetic and sequence analysis of MHC genes and drafted the manuscript. JED isolated BACs and carried out FISH experiments. PC fingerprinted and processed BACs. LW and JH annotated BACs. JK contributed to analysis of TAP genes and generation of TAP transcripts. All authors edited and approved final manuscript, with particular help with figures from LW.
This work was funded by an ARC Discovery Grant to KB and SB, and a Wellcome Trust Grant (084071) to SB. HVS was supported by a University of Sydney Postgraduate Award and a William and Catherine McIlrath Scholarship for travel to the Sanger Institute. JK and HVS are supported in part by Wellcome Trust Programme grant 089305. KB is supported by a University of Sydney Thompson fellowship and an ARC Future Fellowship. We thank Tony Papenfuss and Emily Wong for bioinformatics support.
An update of the HLA genomic region, locus information and disease associations: 2004
HLA and HIV-1: heterozygote advantage and B*35-Cw*04 disadvantage
MHC class I variation associates with parasite resistance and longevity in tropical pythons
Comparative genomic analysis of the MHC: the evolution of class I duplication blocks, diversity and complexity from shark to man
Comparative genomics of major histocompatibility complexes
Molecular dynamics of MHC genesis unraveled by sequence analysis of the 1,796,938-bp HLA class I region
DNA sequence analysis of 66 kb of the human MHC class II region encoding a cluster of genes for antigen processing
Human MHC class III and IV genes and disease associations
Natural History of the Major Histocompatibility Complex
Co-evolution of rat TAP transporters and MHC class I RT1-A molecules
The porcine major histocompatibility complex and related paralogous regions: a review
The gentle art of gene arrangement: the meaning of gene clusters
The chicken B locus is a minimal essential major histocompatibility complex
Mapping of mhc class I and class II regions to different linkage groups in the zebrafish, Danio rerio
Ancestral organization of the MHC revealed in the amphibian Xenopus
Co-evolving genes in MHC haplotypes: the "rule" for nonmammalian vertebrates?
Gene organisation determines evolution of function in the chicken MHC
Reconstructing an ancestral mammalian immune supercomplex from a marsupial major histocompatibility complex
Marsupial MHC class II beta genes are not orthologous to the eutherian beta gene families
Analysis of a marsupial MHC region containing two recently duplicated class I loci
Evolution of the opossum major histocompatibility complex: evidence for diverse alternative splice patterns and low polymorphism among class I genes
Radiation of extant marsupials after the K/T boundary: evidence from complete mitochondrial genomes
Class I genes have split from the MHC in the tammar wallaby
MHC-linked and un-linked class I genes in the wallaby
High levels of genetic variation at MHC class II DBB loci in the tammar wallaby (Macropus eugenii)
Molecular cloning and characterization of the polymorphic MHC class II DBB from the tammar wallaby (Macropus eugenii)
The simple chicken major histocompatibility complex: life and death in the face of pathogens and vaccines
Major histocompatibility complex gene mapping in the amphibian Xenopus implies a primordial organization
The delayed rise of present-day mammals
Disruption and pseudoautosomal localization of the major histocompatibility complex in monotremes
Identification and sequence analysis of an Mhc class II B gene in a marsupial (Monodelphis domestica)
Selection, diversity and evolutionary patterns of the MHC class II DAB in free-ranging Neotropical marsupials
Novel alleles in classical major histocompatibility complex class II loci of the brushtail possum (Trichosurus vulpecula)
Characterization of major histocompatibility complex class I and class II genes from the Tasmanian devil (Sarcophilus harrisii)
High levels of genetic variation at MHC class II DBB loci in the tammar wallaby (Macropus eugenii)
MHC class II genes of a marsupial, the red-necked wallaby (Macropus rufogriseus): identification of new gene families
Influence of allelic polymorphism on the assembly and surface expression of class II MHC (Ia) molecules
The properties of the single chicken MHC classical class II alpha chain (B-LA) gene indicate an ancient origin for the DR/E-like isotype of class II molecules
A centromere-specific retroviral element associated with breaks of synteny in macropodine marsupials
Evolutionary dynamics of non-coding sequences within the class II region of the human MHC
Tracking marsupial evolution using archaic genomic retroposon insertions
OligoSpawn: a software tool for the design of overgo probes from large unigene datasets
High throughput fingerprint analysis of large-insert clones
Contiguation of Bacterial Clones
Contigs built with fingerprints, markers, and FPC V4.7
Complete MHC haplotype sequencing for common disease gene mapping
Evolution and comparative analysis of the MHC Class III inflammatory region
Bioedit: A user friendly biological sequence alignment editor and analysis program for windows 95/98/NT
MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment


text:
How rich is Marcus T. Paulk?
Marcus T. Paulk Net Worth:
$500 Thousand
|Birth date:
|October 12, 1986
|Birth place:
|Los Angeles County, California, United States
|5 ft 10 in (1.78 m)
|Actor, Rapper, Dancer
|United States of America
Marcus T. Paulk net worth, wiki & biography:
Marcus T. Paulk Net Worth $500,000
Marcus T. Paulk began his career when he was a toddler, doing road performing on Venice Beach. He went on to do voice-over work for the animation Hammerman. He had cameo roles in movies such as Nothing to Lose and One Night Stand. His character as Myles Mitchell to the television series Moesha was the turning point of his career as an actor. He was the national representative for the Children Are Paramount effort in 1997. He also appeared in Roll Bounce,Another Cinderella Story, and Take the Lead. Marcus Terrell Paulk was born on October 12, 1986 in LA, California, USA.


text:
Joe Greg Rucker, 50, of Rustwood Drive, was arrested Thursday night after an officer chased him for a traffic violation. He was later found to be a convicted felon in possession of a firearm, Athens-Clarke County police said.
An officer was stopped for a red light at Lumpkin and Broad streets at about 9:25 p.m. when Rucker drove a car through a red light right in front of the officer, police said.
The officer tried to conduct a traffic stop, but Rucker kept driving until he got to the Rocksprings Homes housing complex, where he got out of the car and ran, according to police.
The officer nabbed Rucker after a brief foot chase, police said, and recovered a loaded pistol that Rucker had tossed along the way.
Rucker is a career criminal who has served six separate prison terms beginning in 1992, according to the Georgia Department of Corrections. He went to prison for convictions in Athens-Clarke County for drug, burglary, firearms and other offenses.
Rucker was charged Thursday night with possession of a firearm by a convicted felon, obstruction of a law enforcement officer, fleeing or attempting to elude a police officer, driving on a suspended license, and failure to obey a traffic control device.


text:
Housekeeper in Middle River
House Cleaning offered in Baltimore County, MD. Any size job is welcome. Call for estimate. One Time or Frequent Visits are Welcome! Experienced cleaners ready to keep your house spotless. Extra charge for pets. Price based on house size, # of people/children, frequency of cleaning, and number of tasks. Call 443-600-... for estimate. KLG Cleaning
Housekeeper Details
|Email Address
|Phone Number
|443-600-... Confirmed
Bathroom Cleaning
Kitchen Cleaning
General Room Cleaning
Change Bed Linens
Oven Cleaning
Refrigerator Cleaning
Surface Polishing
Take Out Trash/Recycling
Notes made here will only be seen by you. If you want to message the owner of this listing
More in Middle River, Maryland
Looking for Housekeeper jobs in Middle River, MD?
Find available jobs in your city.
Featured Housekeepers near Middle River, MD


text:
Wave Brazilian Virgin Hair 2/3/4 Bundles Brazilian wave Hair Brazilian Hair Weave Bundles Brazilian Virgin Hair Straight Gift
|45 days Money back Returns accepted if product not as described, buyer pays return shipping; or keep the product & agree refund with seller.
On-time Delivery
Guarantees: On-time Delivery 60 days
|Product Information
|100% Human Hair
|Brand Name
|7A Brazilian Virgin Hair Body Wave
|Item Type
|Human Hair Extensions, Hair Weaves, Human Hair Weaving, Hair Bundles
|Can be curled / straightened
|4 Dyeable And Bleachable – Not Expeed 180C/356F
|Products color
|Natural Color #1B (Others #1, #2, #4, #6, #12, #18, #27, #613 Available contact me if you need these color)
|12-22 inches avaliable
|Bundles per Package
|One Single Bundle; 3pcs/Lot; 4pcs/Lot; 5pcs/Lot; 10pcs/Lot; Mixed And Wholesale
|Hair advantage
|1, Tangle Free, Minimal Shedding, Please Comb It Carefully
|Hair advantage
|2, No Lice, No Chemical, No Smell, No Split Ends, No Synthetic Fibers
|Hair advantage
|3 Soft, High In Luster, Healthy Thick Bundles, Full Ends
|2, No Lice, No Chemical, No Smell, No Split Ends, No Synthetic Fibers
|Brazilian Virgin Hair, Peruvian Virgin Hair, Malaysian Virgin Hair, Russian/Filipino/Indian/Cambodian/Mongolian virgin hair, etc.
|We Can Accept Return If The Hair Is Un-used Or It Is Original State.
|Body Wave,Silky Straight, Kinky Curly, Loose Wave, Natural Wave; deep wave
|Shipping term
|2-3 Working Days to USA 3-5Working Days to Russian Federation DHL also available and it only take 2-5 days. Estimated shipping time Based on the Different countries
|BODY weave or Silky Straight Clip in Virgin Hair Human Hair Extension
|Processing time
|Your payment will be taken 24 hours for confirmation,After this we will ship your order within 48 hours
Yes, If your order quantity for each color each size more than 1 kilogram, Please contact us
| Wholesales, OEM, Customized Service
One stop solution to help your business grow up, we offer competitive price for wholesales customer, and Package design, Special customized order according to your
| 4
Natural ShIne,health,Soft and Bouncy . Blend Well with Natural Hair,thick FuLL Head
100 grams Per Bundles
|100% real picture shot by Camera! Why color difference exits It is unavoidable for color difference on displays during online shopping. Buyers use different display devices and different displays show colors a little different. Settings and brands varies with colors on display. About color difference: Some pictures may look a little different from the real products because of some objective factors such as computer showing, weather and light. Please believe in the real products when there is inconsistence between photos and real products.
The longer the thinner,such as 100g will can make full head for 18inches hair extensions, but you will need 140g for 22inches length
|China Post Air Mail
|Delivery Time (working days & not including holiday)
|United States
|Russian Federation
|United Kingdom
|Saudi Arabia
|Other Countries
| 100% Original virgin hair,7 days warranty supports
All our hair is Original virgin hair, which has been inspect by CISS. If there is any quality problem after received, it can be returned within 7 days.responsible for your project, so we will do everything from the beginning to guarantee accomplishing your project successfully from design to installation, you take no risk of your money, time and you can get a perfect solution.
|Customer satisfaction is always our goal,after get the hair,if you love it,please kindly give us your positive 5-star feedback.Your positive feedback will much appreciated,it is our motive power that continue to supply high quality hair to customers. Please contact us before leave negative feedback.we understand the concerns and frustration you might have and will try our best to resolve the issues.please give us the opportunity to solve the problem.Thank you. Dispute is not the best way to solve problems.i know that we can't make everybody satisfied,if you dislike hair or the hair has something wrong,you can tell us first time,and we will be here always.we are very glad to help you to solve your problems.
| 1. Order Closed By Security Reason Please provide the proof requested by Ali-express or contact us for immediately to help you solve it. 2. Shipping Address Reminder 1.Express doesn't accept any address with P.O.Box
2.Please Ensure Your Address Is Correct,Changing Address After Parcel Left Factory Will Cause Extra 10$ When Deliverred.
Package Lost Irresponsible :
Shipping address is incorrect,DHL wont able to find It.nobody be able to sign the packge,DHL leaving at door,package stolen signed by someone else you do not know which means malicious stolen.
Identification test:
Chemical fiber hair after combustion can harden,hair like plastic after burning all stick together a massive;after burning and the real hair will become powder,can be crushed.
Please Make reference the Link as following : How to install clip in human hair extensions
|To maintain the hair at its best, please follow the instruction carefully,These tips can help you keep human hair extensions looking fabulous and lasting longer: 1.keep the hair clean by using a moisturizing or deep cleansing shampoo. 2. Before washing,comb the hair gently to detangle by using a wide teeth comb. 3. Gently shampoo with lukewarm water in a downward motion,working shampoo from top to bottom. 4. Conditioning the hair is vital to its maintenance. Condition the hair with a moisturizing conditioner in the same manner as the shampoo. 5.Squeeze out excess water and air dry. Air drying preserves the hair more as opposed to blow drying or use of heat. 6. Always consult a professional regarding the chemical procedures,such as dying and bleaching.
Q 1: Why Are My Hair Extensions Getting Tangle ?
A: Your Hair Extensions can tangle due to dryness, oil & dirt build-up, salt water, chlorine and not combing ( wide tooth comb ) out your hair daily Make sure to wash & condition your hair at least once a week, twice a week is better. Use hydrating drops or consult your stylist for nore help.
Q 2: Why Are My Hair Extensions Getting Shedding ?
A: Firstly, PLS running the with your fingers or Wide-toothed gently, to prevent the shedding.For curly hair, pls don't use the comb. Secondly, After wash pls nurse the hair with some hair oil, then the hair will be silk and soft.
Q 3: is there any customs tax?
A: usually we will put lowest amount in the invoice to avoid custom tax as possible as we can,but due to different countries(especially in south America and Africa),there migth be some custom tax which is not considered in the price and should be paid by buyer,you could leave a message when placing the order about how much you want us to put on the invoice value.
Q 4: what is our return policy?
A: if the hairs have some quality problem,we will accept the returning within 7 days after finished the order,and we will pay for the shipping fee not over than $20.if you don't like the hairs,but there is no quality problem,you can also return the hairs within 7 days,but you should p[ay for the shipping fee
Hair Advantaged
1.No Shedding, No Tangle ,No Lice,Double Strong Weft
2.Full Cuticle Hold, Soft,Thick,Can Be Last One Year
3.No lice,no bad smell, keep wave after Wash, Brazilian Hair Bundles
4.Can be dyed and bleached, Brazilian Virgin Hair
Hair Style:Cheap Brazilian Body Wave 4 Bundles
Hair Length:6 inch to 28 inch in our store, Can Be Customized Brazilian Hair
Hair style:Straight, Body, Natural Wave, Deep, Kinky Curly, Water Wave
Hair Weight:100g/bundle, Mink Brazilian Hair,Brazilian Hair Weave Bundlles
Delievery Time: Fast free shipping by Fedex or DHL,about in 2-4 working days after shipped
0 out of 5 (0 Ratings)
|Positive (0 %)
5 Stars(0)
4 Stars (0)
|Neutral (0%)
3 Stars(0)
|Neutral (0%)
2 Stars(0)
1 Star(0)
|Shipping Company
|Shipping Cost
|Estimated Delivery Time
|3 - 7 days
|3 - 7 days
|5 - 14 days
|Post Air Mail
|Free Shipping
|15 - 45 days
|Return Policy
|If the product you receive is not as described or low quality, the seller promises that you may return it before order completion (when you click "Confirm Order Received" or exceed confirmation timeframe) and receive a full refund. The return shipping fee will be paid by you. Or, you can choose to keep the product and agree the refund amount directly with the seller.
N.B.: If the seller provides the "Longer Protection" service on this product, you may ask for refund up to 15 days after order completion.
|Seller Service
|On-time DeliveryIf you do not receive your purchase within 60 days, you can ask for a full refund before order completion (when you click "Confirm Order Received" or exceed confirmation timeframe).
Wholesale Brazilian Hair Virgin Lace Front Ponytail Wigs Glueless Natural Straight Full Lace Human Hair Ponytail Wig In Stock
7A Short Full Lace Human Hair Wigs For Black Women Silky Straight Brazilian Full Lace Wig With Baby Hair Glueless Lace Front Wig
Top New Brazilian full lace human hair wigs Full Lace Front Wig Natural straight wigs for black women with baby hair in stock
$ 99.00 / piece
Brazilian Virgin Hair Straight Human Hair 1PCS Human Hair Extension No Tangle and Shedding Fast Shipping Brazilian Straight Hair
$ 23.81 / piece
2017 PREDAZZLE 10A Brazilian Lace Front Human Hair Wigs Perruque Straight Virgin Baby Hair Glueless Wig for African Americans
$ 165.69 / piece
Peerless Virgin Hair Brazilian Straight Lace Closure Bleached Knots, 8A Virgin Brazilian Hair Closure Soft&Silky Straight 8″-20″
$ 36.00 / piece
online shopping, buying directly from manufacturers?
- Go to Alibaba.com and enter the wanted item into search field.
- Having made your choice, click "Contact supplier" button on the right
- On the next page enter your request and click "Send" button.
- Upon clicking you will be asked to get registered. Do it and wait for the reply.


text:
Barrel Prices are Dropping Whites Case
Your Barrel Prices are Dropping Whites Case includes two bottles each of
Villa Amoroso Moscato Vino Dolce
The sunny days and cool nights of northern Italy's Lombardia province nurture grapes, resulting in flavorful, festive wines like Villa Amoroso Moscato. Made from the Muscat grape, this spritzy and light-hearted white has a sweet, floral feel that can bookend any meal, whether you serve it as an apertif or with dessert. Moscato is beloved for its sweet peach and citrus flavors, and this bottle won the Gold Medal in the 2016 Winemaker's Challenge International Wine Awards.
Snickerdoodle Chardonnay
Crisp, refreshing chardonnay is the first thing that comes to mind when most people think of white wine. Snickerdoodle makes a fresh Chardonnay, with flavors of green apple, nectarine and juicy melon. A subtle hint of oak adds complexity without cutting into the wine's crispness, resulting in a delicious gem of a California Chardonnay.
Big Cirrus Chardonnay
Chilean Chardonnays are picked earlier, capturing a green essence that sets them apart from their California counterparts. Big Cirrus flaunts vibrant green apple and citrus zest flavors with a clean and smooth finish that lingers on the palate. If you prefer crisper Chardonnays, Big Cirrus Single Estate is your next white wine.
Kiyapura Sauvignon Blanc
In 2005, Wine Enthusiast Magazine named Chile's Colchagua Valley Wine Region of the Year. Since then, Chile has become a hotspot for wine, producing smooth and well-rounded whites like this Camartina Estate-bottled Chardonnay. It's got moderate acidity, clean minerality, and notes of tropical fruit and lean pear. Enjoy it with full-bodied fish, white meats, creamy seafoods and corn-based dishes.
GrooVee Gruner Veltliner
GrooVee Gruner Veltlinier is bone dry and light gold in color. Aromas of green apples and gooseberries fill the nose while, on the palate, it is balanced by a delicate fruitiness. This is a fresh, zingy wine that shows crisp acidity, making it a perfect food wine. Served lightly chilled, this Gruner Veltliner is perfect on its own as an aperitif and also a wonderful accompaniment to seafood or white meat dishes.
Vina San Juan White Blend
La Mancha is the land of Hillside casas, Don Quixote, and truly special white wine like Viña San Juan's blend of Chardonnay, Verdejo, and Viura. This elegant Spanish white has wonderful, fresh aromas of herbs, apple, peach, and tropical fruit, which reflect on the palate and lead to a lovely, silky finish. This makes for a perfect pairing with tapas and a range of Spanish seafood dishes including fish with butter sauce, pasta and cheese.


text:
It's parent-kid's day at Freya's kindergarten. So, the school invited parents and their child to take a 5-hour trip on weekend.
We boarded the Gyeonchun line that took us from Seoul to Daesong-ri.
Moms and Dads were busy making sure that the kids were alright during the ride.
well..we DID NOT get any seats!!!
gosh..weekend hikers occupied most space:-)
But it didn't hinder us from enjoying the ride. In fact, the school had provided each kid with a map so as to keep on alert where they were. On each station, they got to post up a sticker on each station we had passed.^^ what a way to keep the kids occupied.
Eyra, too, was so eager to make sure that she didn't miss even a single station we're passing by.......oh, well.
Then, we arrived at around 9.30 and had enough time to play in front of Daesongri station--while waiting for other groups to arrive.
Not a minute too soon, 10 o'clock was the time when most parents and their kids arrived. What a commotion.
Okay, keep the story short: we went on a forest or well..bush-walking actually.
We had one guide to tell us what we're looking at.
In short: todays' topic was that we learn about nature--even the trivial ones.
We're walking towards a river. Yes, a river called 북한강 - Bukhan River where most of our activities were held.


text: Well Firstly I arrived at the hotel at 7 am with my travel Buddy. We were hoping to use their luggage storage room for a few hours until the proper check in time. But they just asked us to have a seat in the Lobby for about 10 mins while they readied a room for us. Considering the mammoth Journey... More


text:
Monday, September 17, 2012
Yodle Opens New Austin Office, Has Massive Hiring Plans
Yodle, a local online marketing firm, said today that it has moved into a new, 100,000 square foot office it has leased in Austin, and expects to hire "hundreds" in the location. Yodle, which helps local businesses market themselves online, said the new office will accomodate its current 375 Austin employees, and that it expects to brings its staff to more than 750 employees over the next five years. Yodle originally set up shop in Austin in May of 2011, when it acquired ProfitFuel.


text:
Thursday, November 4, 2010
Matarani Mounamidi Audio Release Function
Prithviraj - The Actor [Official Fans Page] http://www.facebook.com/actorprithvirajofficial
Join Here : http://www.orkut.com/Main#Community?cmm=6136651 [Official Orkut Community of Prithviraj]
Prithviraj on Twitter : http://twitter.com/lionheart_ps
Follow Us : http://twitter.com/PrithvirajBlog
1 comment:
Hai,i am from Tamilnadu.Few days before only i gothrough ur website and blog. It is nice and good to see.I watched all ur tamil films. Recently i watched few of ur malayalam movies through online eventhough i am not familiar with the malayalam language.Your way of portraying and presenting the roles,voice modulation,language pronounciation(tamil in mozhi),acting style is really good,admirable and inspirable. Keep it up always.I wish u all success in ur carrier. Do more films in tamil also. Belonging to be an indian, i wish u a belated diwali wishes to u(kindly forgive me not delivering advance wishes). Convey my wishes and regards to ur family members. Take care. Have a nice day always.I hope hereafter we are being friends if u like.Finally,Glad to write a comment on ur guestbook(first time in my life to an actor) and thanks for you to give a chance. Hope it will continue.If you wish and find time reply me through my email id.Bye now.ReplyDelete


text:
Pupil Personnel Services
The Brewster Central School District is committed to educating students with disabilities within its own schools whenever appropriate. Special Education services are available to students between the ages of 3-21. Click on Programs to see a full listing of the programs and services available.
What to Do If You Have Concerns about Your Child
For Parents of Pre-School Children:
If you have concerns that your preschooler is not reaching the developmental milestones or performing as you would expect:
- discuss your concerns with your child's pediatrician
- if your child attends a preschool or nursery school program, share your concerns with your child's teachers
- if you and the professionals working with your child continue to suspect that your child's development is being compromised by a possible disability, please contact:
Ms. Jacqueline Smallwood
Elementary/Pre-K Special Education Administrator
Brewster High School
50 Foggintown Road; Brewster, NY 10509
845-278-8570 ext. 1361
She will explain the process of referral to the Committee on Preschool Special Education (CPSE) and help you to identify other avenues of support should a referral not be indicated at this time.
For Parents of School-Age Children:
If you suspect that your school-age child may have a disability which might be impacting their performance in school, you are encouraged to speak with your child's teachers and/or building support staff who know your child. If together, you continue to feel that your child is not performing at a level similar to their peers, ask about the building level supports or resources which have been or may be available to assist your child.
Public Schools are required to provide students who are struggling in school with appropriate interventions to address areas of need. The student's response to these interventions is closely monitored and interventions are adjusted as needed. This process of providing scientifically researched interventions to students who are struggling; monitoring their progress and adjusting the interventions if needed, is known as "Response to Intervention" or " RTI". RTI is referenced in the most recent revision of the Individuals with Disabilities Education Act (IDEA), the Federal law which governs special education services to students with disabilities ages 3-21.
If a child fails to progress academically despite a comprehensive, coordinated and well monitored plan of interventions, the building level support team, known as the Instructional Support Team (IST) or Student Study Team (SST,) may recommend a referral to the Committee on Special Education (CSE). You will be notified if a referral has been initiated and you will also receive a written acknowledgment of the referral from the Office of Special Education. Your written consent will be needed to determine if your child has a disability. As a parent, you may directly refer your child to the Committee on Special Education (CSE) should you have concerns that an educational disability could be impacting school performance. The evaluation process consists of the following mandated evaluations:
- Psychological Evaluation
- Educational Evaluation
- Social History
- Copy of Physical Examination
- Current Teacher Reports
- Other Evaluations which may be recommended: (Speech & Language evaluation; Fine and Gross Motor testing; Psychiatric Evaluation; etc.)
When the evaluations are completed, the Committee on Special Education (CSE) will schedule a meeting to review the evaluations and determine if your child has a disability as defined by Federal and State laws and regulations. The membership of the CSE is mandated by law and includes, among others, the child's parent or guardian. After reviewing all evaluations and reports, the CSE must determine if the student presents with an educational disability in one of 13 categories outlined in law. If the student is found to be eligible for special education programs and services due to an educational disability, then a document called an "Individual Education Plan (IEP)" will be generated which describes the kind of special education programs and services which are recommended to address the student's areas of need. The Committee may also determine that a student is ineligible for "classification" and may recommend other building levels services to support the child.
If after discussing your concerns with your child's school staff, you have questions about the CSE referral and evaluation process, please contact:
For Elementary Students (Grades K-5):
Ms. Jacqueline Smallwood
Elementary/Pre-K Special Education Administrator
Brewster High School
50 Foggintown Road; Brewster, NY 10509
845-278-8570 ext. 1361
*For more information please see the link to the New York State Education Department's publication: "Parents' Guide to Special Education" which is available in both English and Spanish and which can be found on the "Forms & Procedures" Tab on the Special Education Webpage.
Programs & Services
Whenever possible, students with disabilities will be educated in their home school. Special education services are available to students between the ages of 3-21. The following services/programs are available in the Brewster Central School District:
Students between the ages of 3 to 5 who are classified as preschool students with disabilities by the Committee on Preschool Education (CPSE) may receive special education related services and/or programs. Related services such as: speech therapy; counseling; occupational therapy; or physical therapy; and programs such as: special education itinerant services (SEIT), integrated classes and special classes; are all part of the continuum of special education programs which are available through State and county approved agencies and preschools. Special education preschool programs and services are recommended through the Brewster CPSE and administered by the Putnam County Department of Health.
- Speech and Language Therapy
- Occupational Therapy
- Physical Therapy
- Counseling
- Teacher of Hearing Impaired
- Teacher of Visually Impaired
- Nursing Services
These are services provided by a special education teacher either directly to the student who is in a general education program or indirectly to the student's regular education teacher(s). Direct services are specially designed individualized or group instruction that is provided a minimum of two hours per week to aid the student to benefit from general education classes. Indirect service is consultation provided by a special education teacher to general education teachers to assist them in adjusting the learning environment and/or modifying their instructional methods to meet the individual needs of a student with a disability who attends their classes. At the secondary level, Consultant Teacher services may be provided in the following support settings:
- BHS: Academic Support Lab
- WMS: Learning Center
Students with disabilities receive instruction within a regular education classroom with nondisabled peers. Instruction and support is delivered collaboratively by both the regular and special education teacher. These classes are available at all levels in all school buildings on a grade level or subject specific basis.
SPECIAL CLASSES (Self-Contained Class)
Students with disabilities receive specialized instruction in a separate location in small groups on a per subject or part-time basis. Instruction is delivered by a special education teacher. Students at the high school level may receive credit towards a local/regents diploma.
Students with disabilities who need more intensive services or programs not available in the home school may be educated in approved special education programs outside of the district. The continuum of placements in least restrictive order is as follows:
- Other Public School District
The Committee on Special Education (CSE) may apply on behalf of a student with disability for placement consideration within a special education program operated by another local school district.
- Putnam Northern Westchester PNW BOCES & Southern Westchester BOCES
Putnam Northern Westchester BOCES & Southern Westchester BOCES. BOCES special education programs and classes are located in local public school districts and on the BOCES campus. Depending on the program and the location, students may or may not be integrated with typically developing peers within general education programs.
- Special Act School District
These districts were set up by a special act of the New York State Legislature. Although they are distinguished from approved private special education schools, they are similar in structure. Each of the Special Act School Districts has its own Board of Education which regulates district policy and procedures. Currently, there are 13 special act school districts across the State.
- State Supported or State Operated Schools
New York State Supported and State Operated Schools are special education schools which provide specialized instruction for students with specific disabilities.
- Approved Special Education Private School
On occasion, students may have educational needs that cannot be met in a public school or in a BOCES program. The school district may then apply for placement in an approved private day school.
- Approved Special Education Private Residential School
When the structure of an approved private day school is not sufficient to educate and safely manage a student, the Committee on Special Education (CSE) may recommend placement in an approved special education private residential school. This can occur only after all other options have been explored.
Brewster High School
50 Foggintown Road
Brewster, NY 10509
Director of Pupil Personnel Services
Elizabeth Kennedy
845-278-8570, ext. 1371
Assistant Director of Pupil Personnel Services
Amy Osooli
845-278-8570, ext. 1369
Assistant Director of Pupil Personnel Services
Joshua Loeffel
845-278-8570, ext. 1361
Elementary/Pre-K Special Education Administrator
Jacqueline Smallwood
845-278-8570, ext. 1361


text:
With a market cap of has a large market cap size. Banco de Chile (NYSE: BCH) has been on the stock market since its IPO date on the 8/2/2002. Banco de Chile is in the Foreign Regional Banks industry and Financial sector. Average volume for Banco de Chile, is 40.14, and so far today it has a volume of 6511. Performance year to date since the 8/2/2002 is 22.42%.
To help you determine whether Banco de Chile is undervalued the following values will help you decide. P/E is 12.4 and forward P/E is 12.25. PEG perhaps more useful shows that Banco de Chile has a value for PEG of 1.7. P/S ratio is 3.53 and the P/B ratio is 2.58. The P/Cash and P/Free cash flow is 1.79 and *TBA respectively.
At the current price Banco de Chile is trading at, 67.95 (0.13% today), Banco de Chile has a dividend yield of 4.27%, and this is covered by a payout ratio of 56.40%. Earnings per share (EPS) is 5.47, and this is looking to grow in the next year to -1.30% after growing -4.80% this past year. EPS growth quarter over quarter is 14.30%, and 28.50% for sales growth quarter over quarter.
The number of shares outstanding is 162.32, and the number of shares float is 39.36. The senior management bring insider ownership to 87.90%, and institutional ownership is at 3.20%. The float short is 0.37%, with the short ratio at a value of 3.59. Management has seen a return on assets of 1.90%, and also a return on investment of 8.10%.
The ability for Banco de Chile, to deal with debt, means it current ratio is *TBA, and quick ratio is *TBA. Long term debt/equity is 2.66 and total debt/equity is 2.66. In terms of margins, Banco de Chile has a gross margin of *TBA, with its operating margin at 47.70%, and Banco de Chile has a profit margin of 28.70%.
The 52 week high is -2.71%, with 29.56% being its 52 week low. The 20 day simple moving average is 5.29% and the 200 day simple moving average is 13.08%.
Disclaimer: Remember there is a risk to your investment, this is not a recommendation, nor personal advice, never invest more than you are able too loose.


text:
All of our jewelry is Made in the USA using modern technologies and our unique industrial inspired finishes. My jewelry designs are inspired by the modern, and sometimes aging world around me. I like to take objects from our everyday environment, interpret them in simplistic and interesting ways and share them as a reminder of how unique, beautiful and precious our world really is.
With our Design Your Own items, and custom designs we are opening a creative window for others to express themselves in their own unique way. With our guidance you can create special one of a kind meaningful gifts for yourself or for others.
One of the ways that we are sharing and spreading our vision is through our fun home parties where you can shop our pre made designs in a low pressure environment, or work with us to create your own work of art!
We thank you for all of your support!
Audra Azoury


text:
May 12, 2014
New York has updated its list of trucks that are eligible under its Voucher Incentive Program, a $19 million "first come, first served" program that aims to accelerate the deployment of alternative fuel vehicles and diesel emission control devices for medium to heavy duty commercial fleets in the state.
By promoting the use of advanced vehicle technologies, the New York Trucks VIP will help reduce diesel emissions and promote a more fuel independent state, according to the program's website.
"New York customers are already seeing the benefits of our 195h diesel electric COE vehicles realizing up to 30% fuel savings over diesel-only products in well matched conditions," said Glenn Ellis, Hino Trucks vice president of marketing. "By utilizing the NYT-VIP, customers can realize an immediate payback on the incremental cost of our hybrid truck, and start to see operational savings on day one."
Hino's 195h and 195h-DC diesel electric cab-over has been approved for the program. A list of all eligible vehicles is on the program's website, that includes such makers as Daimler Trucks North America, Isuzu, Peterbilt and Volvo.
Vendors/dealers that market and sell alternative fuel vehicles and diesel emission control devices can apply for a voucher incentive to reduce the cost to the purchaser. Once the buyer receives the new vehicle or diesel emission control devices at the discounted rate, the vendor/dealer will be reimbursed the full voucher amount.
The NYT-VIP is partnership between the New York State Energy Research and Development Authority, the New York State Department of Transportation, the New York City Department of Transportation, and CALSTART, a group of more than 140 firms, fleets and agencies dedicated to supporting "clean transportation" technology.
We offer e-newsletters that deliver targeted news and information for the entire fleet industry.
Scott Sutarik from Geotab will answer your questions and challenges
Steve Carlson from Xantrex will answer your questions and challenges
First Impressions: Volvo's New VNL Highway Tractor [Video]
The Heavy Duty Trucking Exchange Recap [Video]
On the Spot: Volvo's VNR as a Daycab and Sleeper [Video]
On the Spot: Ride and Drive at HDTX [Video]
Prestolite's Isolated Ground Technology Protects Engines from Stray Voltage
Bike Hotspot Helps Reduce Accidents with Cyclists
E-Zoil Offers Glass Cleaning Solution
DAT Releases Load Board for Small Fleets and Owner-Operators