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Latest Press Story
February 2017
Catholic groups react to Trump's US exit from Trans-Pacific Partnership (NCR Online)
Beyer Joins Dem Colleagues to Introduce Religious Freedom Bill (Falls Church News-Press)
House Delegation Introduces Freedom Of Religion Act In Response To President Trump's Muslim Ban (Alexandria News)
Catholic mass for Muslims draws more than 600 people blocks from the White House (Mondoweiss)
January 2017
550 attend Mass outside White House in solidarity with refugees (America Magazine)
How Should We Respond to the Age of Fake News? by Stephen Seufert (Millennial)
Trump exacts revenge on Kasich with Ohio GOP shakeup (FOX News)
What could Trump's 'first day' agenda hold? (FOX News)
Trump already breaking mold of the presidency (FOX News)
Obama and Pence hit Capitol Hill amid health care fight (FOX News)
December 2016
Pope Francis is waging a war on Christmas. Christians should join him. by Christopher Hale (Washington Post)
House Freedom Caucus already sparring with GOP brass? (FOX News)
Rust Belt Rout: The Forgotten Man (and Woman) Strikes Back by Stephen Seufert (Huffington Post)
TIME Magazine chooses Donald Trump as 'Person of the Year' (FOX News)
November 2016
Trump is saving Carrier jobs...will other companies want in? (FOX News)
Should Nikki Haley be chosen to join Trump's Cabinet? (FOX News)
Should Trump fully hand over his businesses? (FOX News)
Hale: Person who released Clinton emails is a criminal (FOX News)
October 2016
Where Have the Pro-Life Catholics Gone? by Stephen Seufert (Sojourners)
As outrage over lewd comments piles up, Trump struggles among Catholics (Catholic News Service)
How do VP candidates differ on religious issues? (FOX News)
September 2016
Leave It to Trump to Split a Catholic-Evangelical Bloc That's Generations Old by Neil J. Young (Religion Dispatches)
Fr. Warren Hall Q&A: God Loves Me by Stephen Seufert (Huffington Post)
August 2016
St. Augustine's Restlessness and MLK's 'Dream' by Christopher Hale (Sojourners)
How Democrats are winning back Catholics by Jennifer Rubin (Washington Post)
Will Catholic Toomey 'speak to power' on Trump? by Stephen Seufert (The Hill)
Pope Francis Opens the Door for Future Female Deacons by Christopher Hale (TIME)
July 2016
Preaching hatred of Islam does not help Christians by James Zogby (The National)
The Democratic Party Is Not One of Inclusiveness by Christopher Hale (TIME)
Trump-Pence is the Most Anti-Catholic Republican Ticket in Modern History by Christopher Hale (TIME)
The Real Trump Scandal Isn't Plagiarism. It's Racism. by Christopher Hale (TIME)
What Tim Kaine, Donald Trump and Pope Francis Have In Common by Christopher Hale (TIME)
Guidelines Without Love by Stephen Seufert (Huffington Post)
Trump and Santorum -- Promoting an Economy of Opportunity or Exclusion? by Stephen Seufert (TruthOut)
June 2016
Trump met with Christian leaders: so how did it go? by Matt Hadro (Catholic News Agency)
Dr. Dawn Eden Goldstein has been a busy bee! by Mark Shea (Patheos)
May 2016
Paul Ryan's Saving Grace? by Stephen Seufert (Sojourners)
Poll: Should the pope allow women to be deacons? (New Jersey Times)
Women Should Be Deacons in the Catholic Church by Christopher Hale (New York Times)
Joe Biden and John Boehner: Our Faith Inspires Political Compromise by Christopher Hale (TIME)
The 'Quiet Revolution' of Pope Francis by Stephen Seufert (Huffington Post)
With Biden and Boehner, Notre Dame on board with Francis agenda by Christopher Hale (Crux)
Say goodbye to the most Catholic presidential election ever by Robert Christian (Crux)
April 2016
Could Pope Francis be a feminist pope? by Christopher Hale (Washington Post)
Catholic Democrats split on wisdom of Sanders' Vatican drop-in by Michael O'Loughlin (Crux)
Where are Bernie and Hillary? Dems leave NY days before primary by Charley Hannagan (Syracuse.com)
Why is Bernie Sanders in Vatican City not New York City? by Elizabeth Landers (CNN)
Rumors of God's Death Have Been Greatly Exaggerated by Christopher Hale (TIME)
Why Catholics Should Stand Against Donald Trump's Extremism by Stephen Seufert (Truthout)
Tennessee lawmakers named the Bible as state book — and some Christians are furious by Travis Gettys (Raw Story)
March 2016
25 Faith Organizations Ask Appropriators: Eliminate the Immigrant Bed Quota (Friends Committee on National Legislation)
Pope Francis Will Be a Pro at Instagram by Christopher Hale (TIME)
Stated Clerk of the PCUSA Joins 22 Faith Leaders in Call for Congress to Pass Legislation to End the Embargo of Cuba (Office of Public Witness)
#PrayForRefugees: How You Can Change the Worst Refugee Crisis since WWII by Mark Martin (CBN News)
A welcome to strangers in Iraq by Christopher Hale (World Vision)
My Country: The Debate Over Religious and Civil Liberties by Stephen Seufert (Huffington Post)
February 2016
The Pope, the Piggy Bank, and the Constitution by Stephen Seufert (Sojourners)
Mitt Romney says Trump's response about David Duke is 'disqualifying' by Twitchy Staff (Twitchy)
Voting Bernie Sanders? A Rundown and Final Analysis for Catholics by Keith Michael Estrada (Patheos)
Bernie Sanders is wrong: Pope Francis is no socialist by Christopher Hale (National Catholic Reporter)
The common ground between the magistrate and the minister by Christopher Hale (Crux)
Blessings From the Border A Q+A with Catholic activist Christopher J. Hale by Ryan Hammill (Sojourners)
The pope flashes his political passions by Nahal Toosi (Politico)
Twitter tracker gives up the top choices for Lent this year by Lisa Gutierrez (The Kansas City Star)
Pope Francis' Guide to Lent: What You Should Give Up This Year by Christopher Hale (TIME)
Pope Francis's contraception comments highlight the Catholic Church's hypocrisy, not progress by Jaweed Kaleem (Quartz)
Pope Francis suggests contraception could be permissible in Zika fight by Sarah Pulliam Bailey and Michelle Boorstein (Washington Post)
Pope's blessing, call for mercy impacts El Paso crowd by Soli Salgado (National Catholic Reporter)
Immigration Activists Hope for the Francis Effect by Ryan Hammill (Sojourners)
Steering committee forms for LGBT orgs across state by Paige Cooperstein (Philly Gay News)
Trump Reeling After Religious Leaders Condemn His Attack On Pope Francis by Jason Easley (PoliticusUSA)
Morning Catholic must-reads: 18/02/16 (Catholic Herald)
January 2016
LGBTQ Pennsylvania Steering Committee Meeting by Michael Mahler (Erie Gay News)
Pope Francis Reaffirms God's Love for LGBT Community in New Book by Christopher Hale (TIME)
This year's presidential election is a chance for churches to earn back trust of young Americans by Kelsey Dallas (Deseret News)
The Forgotten Dream of MLK by Stephen Seufert (Huffington Post)
Catholic Leaders Stand With Obama On Guns And Demand Republicans Follow His Lead by Sarah Jones (PoliticusUSA)
Gun Control Is a Pro-Life Value by Christopher Hale (TIME)
December 2015
Welcoming the Stranger Q&A: Embracing Immigrants and Refugees by Stephen Seufert (Huffington Post)
The Department of Justice Must Investigate ExxonMobil (350.org)
The Rights of Refugees by David Hollenbach (America Magazine)
What Latin American Catholics Can Teach the American Left by Jocelyn Macurdy Keatts (Huffington Post)
Donald Trump's Anti-Christian Campaign by Christopher Hale (TIME)
Pope Francis's Prayers for COP21 by Jocelyn Macurdy Keatts (Huffington Post)
World Leaders Must Listen to Pope Francis on Climate Change by Christopher Hale (TIME)
November 2015
'I was a stranger, and you welcomed me' by Stephen Seufert (Bucks County Courier Times)
Catholics Back Refugee Resettlement (The Tablet)
The Catholic Millennial: 21 Questions for Christopher J. Hale by Sean Salai, S.J. (America Magazine)
"Yet until brown clay has been rammed down my larynx...." by Michael Sean Winters (National Catholic Reporter)
Faith leaders say refugees from Syria, elsewhere require compassion, acceptance (The Catholic Sun)
There's Nothing Christian About Blocking Non-Christian Refugees to the U.S. by Christopher Hale (TIME)
When it comes to terrorism, 'a Christian is a Christian' by Christopher Hale (Crux)
October 2015
Pope Francis would give a thumbs up to Oprah's 'Belief' series by Christopher Hale (Crux)
Pope Francis' Language on LGBT Catholics Helps Illuminate What's Next for the Church by Stephen Seufert (Sojourners)
Gun Violence and the Common Good by Stephen Seufert (Huffington Post)
Trans-Pacific deal a step closer to final by Soli Salgado (National Catholic Reporter)
Kim Davis' Lawyers Dispute Vatican's Claims That Pope Francis Meeting Was Not an Endorsement, as Speculations Arise That the Pontiff Was Tricked by Char Adams (People Magazine)
Before Meeting Kim Davis, Pope Met With His Gay Friend In Washington by Jaweed Kaleem (Huffington Post)
Pope Francis's Public Transformation on LGBT Rights by Christopher Hale (New Republic)
Pope Francis Met With An Openly Gay Couple The Day Before His Visit With Kim Davis by Ismat Sarah Mangla (International Business Times)
September 2015
LGBT Catholics Confused And Hurt By Pope's Visit With Kim Davis by Antonia Blumberg (Huffington Post)
Pope Francis, immigrants and presidential candidates by Daniel Gunderson (KFGO Radio)
Can Pope Francis sway US politics? (Financial Times)
How Pope Francis resonates with millennials on Weekends with Alex Witt (MSNBC)
What lasting effect will Pope's visit have on US Catholics? (Fox News)
Refugees in Europe, the Pope in America on The Zero Hour with RJ Eskow (Stitcher)
Religion Series: LGBT couples still battling for rights from Church by Abbie Sarfo (The Review)
Papal visit prompts calls for LGBT-inclusive church by Michael K. Lavers (Washington Blade)
Boehner to resign from Congress next month by Tracie Mauriello (Pittsburgh Post-Gazette)
A Different Kind of Crusader by Rob Kunzig (Morning Consult)
Pope for the people galvanizes millennials by Margie Fishman (The Delaware News Journal)
Catholic Church: Listen & Learn On LGBT People by Stephen Seufert (Believe Out Loud)
Why Pope Francis Sounds Like a Democrat by Molly Ball (The Atlantic)
Pope Francis Creates Strange Bedfellows Among Presidential Candidates by Ben Gittleson (ABC News)
By boycotting the pope, US Rep. embarrasses his Church and his country by Christopher Hale (Crux)
The Francis Revolucion by Callum Wood (The Trumpet)
Pope Francis' foray into politics creates discomfort for some Republicans by Alex Leary (Tampa Bay Times)
Activists Hope Pope Can Change Climate Conversation In Washington by Kate Sheppard (Huffington Post)
How Does Pope Francis Influence America's Climate Change Debate? by Lucy Schouten (Christian Science Monitor)
Obama, the Pope, and the President of China Are Teaming Up to Save the World by James West (Mother Jones)
Pope faces tough crowd with climate plea by Timothy Cama (The Hill)
Pope's climate message unlikely to sway Republicans by Andrew Restuccia (Politico)
Why US Bishops Aren't Embracing Pope Francis' Climate Push by Suzanne Goldenberg (Mother Jones)
Pope Francis Faces Challenge Persuading US's Catholic Leaders on Climate Change by Suzanne Goldenberg (The Guardian)
Where Obama, Pope Francis Share Policy Vision: Cuba and Climate Change by Carol E. Lee (The Wall Street Journal)
Pope Francis Is Not 'Progressive'—He's a Priest by Emma Green (The Atlantic)
Washington stirs before dawn for key spots at papal spectacles by Dana Hedgpeth (Washington Post)
The Pope's Funniest Fan: Stephen Colbert by Christopher Hale (TIME)
Catholics Flood 2016 Race by Brendan Kirby (LifeZette)
Catholic congressman says he will boycott Pope Francis over climate change stance by Mike DeBonis (Washington Post)
Troubled Philadelphia Catholic diocese welcomes Pope Francis' late-September visit by Peter Smith (Pittsburgh Post-Gazette)
Joel Hunter, Jim Wallis, and 49 Other Christian Faith Leaders Send Letter to Congress Urging Acceptance of Iran Nuclear Deal (Urban Christian News)
Owning Our Faith Q&A: LGBT Catholics and the Church by Stephen Seufert (Huffington Post)
70+ Organizations Implore Congress to Support Iran Agreement by Win Without Way (YubaNet)
These are Pope Francis's Top 5 Messages for Americans by Christopher Hale (TIME)
Timothy Malcolm: Member of a church should bring its teachings to life by Timothy Malcolm (Times Herald-Record)
Pope Francis is right about Abortion by Christopher Hale (TIME)
The Pope vs. The Donald by Nahal Toosi (Politico)
August 2015
The Top 4 Misconceptions about Pope Francis by Christopher Hale (TIME)
Pope Francis Supports the Iran Deal. So Why Do These Catholic Candidates Oppose It? by Stephen Seufert (Sojourners)
Pope Francis embraces gays, lesbians, but church remains divided over LGBT issues by Ivey DeJesus (Pennlive)
Pope Francis and the Republican Presidential Hopefuls: A Widening Divide by Stephen Seufert (Huffington Post)
Donald Trump has a Pope Francis Problem by Christopher Hale (Crux)
President announces Clean Power Plan to supportive poll results by Patrick J. Foote (People's World)
A declaração do Papa Francisco que mudou a Ingreja em questões LGBTs by Eraldo Luis (Nordeste1)
Africa: Religious Leaders Criticize Obama Comments on LGBT Human Rights by Peter Montgomery (USC Annenberg Religion Dispatches)
Kasich Could be the GOP's Pope Francis Candidate by Christopher Hale (TIME)
John Kasich: Trump's Behavior 'Just Doesn't Seem to Bother Me' by Connor Joseph (NBC News)
July 2015
"The World is Our House": The Gift of a Jesuit Education by Christopher Hale (Jesuit Educated)
Papa Francisco: elemento-chave para as eleicoes presidenciais dos EUA (Instituto Humanitas Unisinos)
The Pope Francis Statement that Changed the Church on LGBT Issues by Christopher Hale (TIME)
Andre Controversa Presents: Same Sex Marriage: Did SCOTUS Overreach? (Blog Talk Radio)
Preaching Pope Francis's Politics May Be the Key to Becoming President by Christopher Hale (TIME)
Church group acts on Pope's climate message by Al Edenloff (Echo Press)
Local faith groups ready for papal visit by Larry Nichols (Philly Gay News)
US Bishops issue bulletin against same-sex marriage by Michael Sean Winters
Pope Francis Isn't Holding Back--And U.S. Politicians Should Watch Out by Christopher Hale (TIME)
Pope Francis in Bolivia: "We Are Tearing Apart Our Common Home" by Stephen Seufert (Sojourners)
Opinion: Democrats must start speaking out about the Planned Parenthood video by Christopher Hale (Washington Post)
'A lot of people want a liver' by Rod Dreher (The American Conservative)
Where are the mainline and progressive voices speaking out after that planned parenthood tragedy? by Ed Stetzer (Christianity Today)
Pope Francis ignites a revolt that will overthrow American capitalism by Paul B. Farrell (MarketWatch)
Pope's climate change statement a challenge for Republicans by David Jackson and Paul Singer (USA Today)
June 2015
Pope Francis's Smaller Visits Have a Bigger Meaning by Christopher Hale (TIME)
Pope's Climate Letter Puts Catholic Candidates on Spot by Andrew Desiderio (RealClearPolitics)
Pope Francis will try to break out of 'the bubble' on his US trip, details show by David Gibson (Washington Post)
3 Ways Catholics Can Respond to the Same-Sex Marriage Ruling by Christopher Hale (TIME)
7 Surprising Ways that Pope Francis' Encyclical is Calling You to Change by Jenny Heipp (Aleteia)
The Pope's Climate Change Call Christopher Hale on The Big Picture with Thom Hartmann (RT News Network)
Pope's Climate Change Statement a Challenge for Republicans by David Jackson and Paul Singer (USA Today)
Papal message pivots to politics of warming by Jean Chemnick (E&E Publishing)
Pope's Encyclical: Authentic Human Development Has a Moral Character by Stephen Seufert (Huffington Post)
Inspring an Ecological Conversion by J. Nick Stevens (Sojourners)
4 Things Pope Francis Wants You to Know about Climate Change by Jennifer Labbadia (Busted Halo)
7 Surprising Ways that Pope Francis' Encyclical is Calling You to Change by Jenny Heipp (Millennial)
The 5 Most Important Points of Pope Francis's Climate Change Encyclical by Christopher Hale (TIME)
Jeb Bush's Response to Pope Francis's Climate Change Encyclical is Hogwash by Christopher Hale (TIME)
Catholic Couple Embraces 'Who am I to Judge' by J. Nick Stevens (Huffington Post)
Tuesday, June 16th: Pope Francis' Encyclical Christopher Hale on The Dom Giordano Show
The Reason Faith Groups Helped Defeat Fast Track by Christopher Hale (TIME)
Why only Putin could turn up late for a meeting with the Pope by Matthew Chance (CNN)
Why are Catholics so critical of consumerism? by Robert Christian (Crux)
Rick Santorum wants Pope Francis to leave science to scientists only when its convenient for him by Christopher Hale (Washington Post)
Pope Francis the Chemist should give congress a lesson by Robert Christian (Time)
What Pope Francis and Joseph Biden Can Teach Us About Suffering by Christopher Hale (TIME)
Santorum to pope: Stay away from science by Stephen Seufert (Philadelphia Inquirer)
Can Pope Francis work a Vladimir Putin Miracle? by Christopher Hale (TIME)
Wednesday June 10th: Climate Change Christopher Hale on The Dom Giordano Show
Jeb Bush thinks his faith shouldn't inform his position on climate change. Except when he does. by Jack Jenkins (Think Progress)
May 2015
Ireland Strikes a Blow For Good-Now What? by Karen Ocamb (Frontiers Media)
Gay Marriage in Ireland isn't a 'No' to Catholicism by Christopher Hale (TIME)
The Catholic Case for Tackling Climate Change by Stephen Seufert (Huffington Post)
Drowning in Debt: Washington and the Unjust Student Loan Crisis by Christopher Hale (Busted Halo)
2016 Candidates Must Step up to the Pope Francis Challenge by Christopher Hale (TIME)
The Source: Religiously Unaffiliated Outnumbering Catholics in Recent Poll by Paul Flahive (Texas Public Radio)
Transgender and Catholic by J. Nick Stevens (New York Times)
2016 Presidential Faith: The Republicans by Christopher Hale (Busted Halo)
Palestinian President Meets Pope Christopher Hale on BBC World News (BBC)
Young people explain what role organized religion plays in their life Christopher Hale on Melissa Harris Perry (MSNBC)
Why are we seeing a decline in religious affiliation? Christopher Hale on Melissa Harris Perry (MSNBC)
How the Church Can Get Millennials Back by Christopher Hale (TIME)
Gun Violence by John Bergen (Catholic Herald)
2016 Presidential Faith: The Democrats by Christopher Hale (Busted Halo)
Kerry Robinson: The Church Deserves Women in Leadership by Jennifer Labbadia (Huffington Post)
Is God in Favor of Immigration Reform? by Lorena O'Neil (Ozy)
April 2015
The Most Catholic Presidential Election Ever by Lorena O'Neil (Ozy)
Pope Francis Would Spare Dzhokhar Tsarnaev's Life by Christopher Hale (TIME)
Fairness in International Trade by Dan Stockman (Global Sisters Report)
Its time for a preferential option for the family by Robert Christian (Crux)
Good Friday and the Modern Version of Jesus Cross by Christopher Hale (TIME)
Transgender Voice of Faith by J. Nick Stevens (HRC)
Can Religious Freedom be used to Discriminate? by Stephen Seufert (Huffington Post)
Analysis: Is Catholic Shift on Gays Substance or Style? by David Gibson (Religion News Service)
March 2015
Catholics, Evangelicals call for end to death penalty by Michael O'Loughlin (Crux)
Cardinal Sean O'Malley by Lorena O'Neil (Ozy)
Pope Francis Has a Woman Problem by Jennifer Labbadia (Huffington Post)
Lenten Reflection Series: Choosing Compassion and Love by Jennifer Labbadia (Millennial)
Fast-Tracking the Trans-Pacific Partnership: Bad for General Welfare and Common Good by Stephen Seufert (Huffington Post)
Pope Francis has Taught the Church to Thrive Again by Christopher Hale (TIME)
I'm Proud to be a Transgender Catholic by J. Nick Stevens (TIME)
As Obama pushes for trade pact fast track, faith groups say to call the deal off by Soli Salgado (NCR Online)
Why Pope Francis is Obsessed with Mary by Christopher Hale (TIME)
New Catholic Group Joins Fight for LGBT Acceptance by Stevie St. John (The Advocate)
Pope Francis has a Woman Problem by Jennifer Labbadia (Huffington Post)
Ministry offers 'Courage' for same-sex attracted, celibate Catholics by Cheryl Wetzstein (Washington Times)
February 2015
Pope Francis Just Showed He's Not Afraid to Part With the American Right by Christopher Hale (TIME)
A new focus on Women's equality with a record number of women in Congress? by Jennifer Labbadia (Millennial)
What Can We Expect from Pope Francis' Address to Congress? by Christopher Hale (Sojourners)
Honoring MLK With Action by Stephen Seufert (Huffington Post)
Freedom misunderstood: Catholic Democrats and abortion by Robert Christian (Crux)
The Missing Faith Voice in the Gun Control Debate by Jennifer Labbadia (Huffington Post)
Pope Francis' Guide to Lent: What You Should Give Up This Year by Christopher Hale (TIME)
Lenten Reflection Series: A Time to Learn How to Love Again by Christopher Hale (Millennial)
What Pope Francis could teach Francis Underwood by Christopher Hale (TIME)
Lenten Reflection Series: Mirroring God's Perfect Love for Others by J. Nick Stevens (Millennial)
Obama invokes Crusades while discussing ISIS Christopher Hale on The Lead with Jake Tapper (CNN)
The Need for a More Pastoral Approach toward Gay, Lesbian, and Transgender Catholics by Stephen Seufert (Millennial)
January 2015
Pro-Choice Mario Cuomo Was Still A Catholic Politician by Christopher Hale (TIME)
Pope Francis' New Cardinals Reflect a 'Poor Church' by Christopher Hale (TIME)
The Environment and Economic Justice: U.S. Catholics Can Take the Lead by Stephen Seufert (Huffington Post)
God calls us to care about the poor, climate by Christopher Hale (NCR Online)
Immigration Reform is a Pro-Life Issue by J. Nick Stevens (Millennial)
Pro-Life is More than being Pro-Birth by Christopher Hale (TIME)
New Catholic Survey Asks About People With 'Homosexual Tendencies' by Jack Jenkins (Think Progress)
An appropriate Catholic Response to the State of the Union by Christopher Hale (NCR Online)
The Catholic Church: Debate Makes It Stronger by Stephen Seufert (Huffington Post)
December 2014
Broadening the conversation on the Catholic family by Stephen Seufert (Philly Gay News)
LGBT Homes Should Open Their Doors to the World Meeting of Families by Francis DeBernardo (Bondings 2.0)
Match Made in Heaven: Catholics Supporting Immigrant and Labor Rights by Stephen Seufert (Huffington Post)
Economic Justice is Essential to Strengthening American Families by J. Nick Stevens (Millennial)
A Nation in Dire Need of Christmas by Christopher Hale (TIME)
If Pope Francis Wrote your New Year's Resolutions by Christopher Hale (TIME)
November 2014
What do Millennial Catholics seek when it comes to LGBT issues? by Bob Shine (Bondings 2.0)
Why Christian groups lead the biggest relief efforts in the world by Christopher Hale (On Faith)
Pope Francis' modern family evolution by Christopher Hale (TIME)
The Saint of 9/11 and the 'Gifts and Qualities' of Gay, Lesbian, and Transgender Catholics by Jennifer Labbadia (Millennial)
An Economy of Inclusion and Solidarity for the 21st Century by Stephen Seufert (Millennial)
October 2014
The New Archbishop of Chicago Is a Radical—And That's a Good Thing by Robert Christian (TIME)
Millenials want a messy earnest discussion about family by Vinnie Rotondaro (NCR Online)
Cutting through the noise: the power of personal relationships by Allison Walter (NCR Online)
The Bishops are catching up to Pope Francis on gay rights by Christopher Hale (TIME)
Vatican document on gays draws cautious mixed response by Willa Frej (MSNBC)
Are we over-hyping the Vatican's new attitude toward the LGBTQ community? by Alicia Menendez (ABC Fusion)
Catholic Bishops urge tolerance toward gays (Arise America)
Weighing a Catholic change for LGBT and Divorcees by Tom Ashbrock (NPR, On Point)
Is the Vatican evolving on sex and marriage? Not the way politicians do by Dave Cloutier (Washington Post)
The Church must lead the fight against violence facing the gay community by Jes Stevens and Christopher Hale (NCR Online)
Churches proceed carefully when encouraging members to get politically involved by Kelsey Dallas (Desert News)
September 2014
The church isn't going away on immigration reform by Christopher Hale (Busted Halo)
Reconciliation as a means to rebuild society by Rachel Malinowski and Christopher Hale (NCR Online)
Is the Catholic Church 'Evolving' on Gay Marriage? by Christopher Hale (TIME)
Supporting Trans Youth: Building a more hopeful future by Jes Stevens (Believe Out Loud)
Obama puts politics ahead of country in latest immigration overhaul delay by Christopher Hale (NCR Online)
Pope Francis's winds of change cross the Atlantic by Christopher Hale (TIME)
August 2014
Pope promotes peace, not pacifism, in Iraq by Christopher Hale (TIME)
Is there a realistic non-violent response to the violence in Iraq? by Christopher Hale (NCR Online)
The Pope isn't a pacifist by Dish Staff (The Dish)
Ferguson: A faithful way forward by Christopher Hale (TIME)
A Church that embraces the cross must embrace politics by Rachel Malinowski and Christopher Hale (NCR Online)
July 2014
After Hobby Lobby: a single-payer health care solution? by Fred Rotondaro and Christopher Hale (TIME)
Mending the Rift Between Obama and Catholics by Robert Christian (TIME)
Is it time to give the boot to employer based health insurance? by Robert B. Doherty (Philly.com)
June 2014
Boehner's got a Catholic problem on immigration reform by Christopher Hale (TIME)
Why tea party Catholicism is a no go by Christopher Hale (TIME)
Pope reportedly receives letter criticizing treatment of LCWR by Joshua McElwee (NCR Online)
Faith groups divided in their reaction to court's decision affirming religious rights by Michelle Boorstein (Washington Post)
Pope Francis gets involved in Middle East peace process by Jerome Socolovsky (Voice of America)
May 2014
Pope Francis' Tweet About Inequality Is the Wake-Up Call We All Need by Robert Christian (TIME)
Pope Francis' trip to the Holy Land will bring attention to global war on Christians by Christopher Hale (TIME)
Why Pope Francis may be the best politician in the world by Christopher Hale (TIME)
April 2014
Cardinals prove borders cannot block the love of Jesus Christ by Christopher Hale (TIME)
Why Pro-lifers Should Join Forces with Senator Kirsten Gillibrand by Robert Christian (TIME)
Why focus on the cross? by Christopher Hale (TIME)
The radical Easter proclamation by Christopher Hale (TIME)
John XXIII and John Paul II, the Catholic Church's modern Saints by Christopher Hale (TIME)
March 2014
Lent is not just for Christians by Christopher Hale (TIME)
Pope Francis' One Big Mistake: Syria by Robert Christian (TIME)
Francis: a Pope that Millennials can love by Christopher Hale (TIME)
Cardinal Sean O'Malley by Lorena O'Neil (Ozy)
Ozy profile of +O'Malley by Michael Sean Winters (NCR Online)
Give 'em Francis, Barry! by Christopher Hale (TIME)
Obama, Francis meet for the first time by Halimah Abdullah (CNN)
February 2014
Remember Benedict the Meek by Christopher Hale (TIME)
Hale remembers "Benedict the Meek" by John McCarthy (NCR Online)
January 2014
Why progressives should oppose abortion by Christopher Hale (CNN)
Pro-life and Progressive by Michael Sean Winters (NCR Online)
December 2013
Rush Limbaugh, Pope is preaching pure marxism by Daniel Burke (CNN)
Catholic group denounces Limbaugh's attack on Pope Francis (Media Matters for America)
November 2013
Groups step in for US Bishops, collects Vatican requested data by Joshua McElwee (NCR Online)
Lay groups luanch surveys to answer Vatican questionnaire by Brian Roewe (NCR Online)
Vatican Downplays controversy over U.S. Embassy move by Dan Merica and Eric Marrapodi (CNN)
Bishops are urged to expand priorities by Laurie Goodstein (New York Times)
Archbishop Kurtz, new head of U.S. bishops group, shares pope's pastoral approach by David Gibson (Washington Post)
U.S. Catholic bishops' new leaders echo pope's concern for the poor by Mary Wisniewki (Reuters)
September 2013
A Christian Prophet by Christopher Hale (America Magazine)
At the Vatican peace in Syria gets a chance by Christopher Hale (Washington Post, On Faith)


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For other Policy Debate postings, including an analysis of the Infrastructure topic follow the links here.
Bering Strait Tunnel
Tunnel Of Love
Case Reviews
Since camp cases are emerging, I have decided to scan through select case Affirmatives and deliver some summaries. While I will be looking at specific camp cases, I intend to summarize the general advocacy without reference to specific files. So, if several camps put out a high-speed rail case, for example, I will generally summarize the case plans, advantages, etc. without referencing a particular file. Now for sure, some of the reviews will be one particular file, if no other camp puts out a similar case.
This series will spread out over several postings as I find time to review the cases. In general, the posts will be short, the comments will be brief and they are strictly my opinions. If I say I do not like a case, it does not mean you should avoid looking at it, especially since sometimes the files are updated and improved.
The case files, I review will be taken from the National Debate Coaches Association, Open Evidence Project.
Bering Strait Tunnel
The case proposes to build a tunnel between the U.S. and Russia under the Bering Sea. The distance is about 65 miles. Presumably the tunnel will support rail traffic as a main source of commerce.
Solves for international cooperation.
U.S. Russian relations.
The claim made is relations are deteriorating although I am not sure they were ever that great. Economically we cooperate. Russia has one of the fast growing economies in the world and that is hard to ignore. Politically we have conflict and have continued to have conflict for decades.
Russian Far East.
The idea here is to increase Russian presence and influence in the Far East. This presence magically deters Chinese expansion, soothes North Korea, etc. I wonder why Russian presence did not deter a nuclear North Korea? I guess because there weren't enough Russian railroads in the region.
Global Integration
Somehow, (I guess because U.S. / Russian relations are solved) this tunnel stimulates this fabulous world-wide cooperation and world-wide cooperation solves a laundry list of nuke war scenarios. One case file spends a lot of cards focusing on interconnection of the electrical grids and suddenly world energy needs are a whole lot closer to being solved. (details like how their 50 cycle system is connected to our 60 cycle system are just, well, details which we can discuss later - like during the 1NC).
Good things
The plan is implemented by land grants. That works, as it has plenty of historical precedence in the American old west. Time frame is fairly good. According to one source the tunnel could be completed by 2019 and return on investment could be rapid afterwards.
Bad Things
One the biggest problems I personally see in this case is no real distinction between solvency and advantages. The harms being solved are then offered as advantages. This doesn't make much sense to me especially since I don't think the case does a very good job of establishing harms in the SQ which requires this tunnel as the way to solvency.
The solvency and indeed much of the success of this case will depend on complete Russian cooperation and there is no assurance that will happen. Many of the advantage cards note the fact that Russian interest in the tunnel is not that great and the U.S. needs to drive the project. In my opinion, the dependency on Russia for success kills solvency and advantages.
In my opinion, dump the Global Integration advantage. World cooperation just seems to spring out of no where. Its fantastic leaps of faith are flights of fancy. (That's a lot of F's)
Topicality is questionable on this one, in my opinion. Fifty percent of the project depends on a foreign government. To avoid T debate, it is argued the U.S. will do the American side and Russia will do the Russian side. Even if topicality is not challenged, that is a huge potential road-block to solvency.
This case needs work. Before building the tunnel, this case needs to build a bridge to link the tons and tons of international coop advantages that somehow magically spring forth.
Tunnel of Love Aff
This case envisions a Bering Strait tunnel, not as a legitimate tool of commerce but instead as an idea which solves real-world harms just by conceptualizing the vision.
It is established in the SQ that American's stereotypical view of Russia and its people creates discourse and ideology that "otherizes" the Russians. This, of course, results in us viewing them as enemies and culturally different and that spills over into real-world conflict.
The plan solves by imagining a world where Russia and U.S. cooperate to build a Bering Strait tunnel. Doing so allows us to bridge the cultural divide. This is posed as a pre-fiat, apriori case which corrects the perceptions which would impede real cooperation. The key to this solvency is gaining the ability to imagine great things and foster Utopian dreams.
Good Things
Some of the cards in the case are excellent and link specifically to the imaginative Bering Strait tunnel.
Bad Things
In my opinion, there needs to be a little more clarity on why this change in mindset needs to be done before real-world cooperation can ensue. I mean, why should the judge think we should not look to a Neg counter-plan which solves real-world, near-term harms while Aff dreams of Utopia? Especially since cultural mindsets tend to change over generations, not years.
I imagine this to be a really sweet Aff. I love the idea of this case. It definitely does a nice job of creating a critical view of the status quo with regard to Russian relations and does offer an alternative. I think if the point can come across that plans will fail without the alternative this case is a pretty good idea. It is not a case for novices to run, however.


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A Pricing Operator-Based Testing Foundation For A Class Of Factor Pricing Models
This paper develops a cross-market version of factor pricing models. It is shown that exact factor pricing holds across two submarkets with respect to their "common factors" if and only if the unique pricing operator for the first submarket is equal to that for the other submarket with probability 1. We define an APT measure as the squared distance between the two pricing operators. Then, testing whether this measure is zero is equivalent to testing exact factor pricing across the two submarkets. Since the estimation of this measure does not require parameterizing and extracting the underlying factors, one can test factor pricing models without knowing any factors. In addition, we present a randomization procedure so that one can use it to conduct a more comprehensive investigation on the empirical robustness of factor pricing models. Copyright 1994 Blackwell Publishers.
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Volume (Year): 4 (1994)
Issue (Month): 2 ()
Pages: 121-141
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Ask TheStreet is designed to answer questions about the market, strategies, and investment methods. Please email us to ask a question, but keep in mind that we cannot offer specific investment advice.
Reader: Please help me to understand how P/E ratios are established. If a company earns X in a given year, how does the market determine the company's future earnings potential? And, if a company reports somewhat disappointing earnings in a given quarter, how are the adjustments to potential growth calculated? --P.D. Gregg Greenberg: Your first question is the easiest to answer because it's just a matter of simple math. After that, things, as they tend to do on Wall Street, get a bit fuzzy. A company's P/E, or "price-to-earnings" ratio, is established by dividing the current share price by the annual earnings per share. Some investors prefer to use the "12-month trailing" earnings in the denominator, because that's actual earnings, while others prefer using "forward" earnings estimates. It's in the use of forward earnings estimates where things start to get hairy. Forward estimates are educated guesses made by Wall Street analysts as to a company's earnings per share for an upcoming quarter or fiscal year. The analysts' guesses can be considered "educated" because they are often guided by the companies themselves. Each Wall Street analyst creates his or her own set of forward estimates, then a service like Thomson First Call takes the average of the expectations. Finally, on D-day, when a company reports its actual earnings for the quarter, both the analysts and the company are put to the test. If the company's profits fail to match or beat the average earnings estimate, the stock usually gets creamed. That's called a "negative surprise." If a company "beats" estimates it's called an "upside surprise" and the stock more often than not heads higher. (If a particular analyst is way off in his estimate, and continues to be wrong quarter after quarter, then you may want to look for another analyst, and he may want to look for another job.)


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Smart watches and exercise watches are the latest up and coming tech trend. However, it is not only the technology powerhouses that are delivering products, even fashion and gear brands are getting in on the act. Surfing and outdoor clothing company RIP Curl has slapped its name on cars in the past, but now the company has developed its own piece of wearable tech, a GPS watch.
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March and April begin our "awakening" after winter's cold. New daylily varieties are ordered. The tractors come out of the barns to be tuned up and tested. Mower blades are sharpened, tools oiled and organized and crew schedules are set.
Mid April is planting time for 6000 pine and spruce seedlings. All our planting is done by hand on a grid system to make mowing easier.
The farm opens in April for field trips, gardening classes, scout troops working on merit badges and writers workshops.
May starts the mowing season on the farm. The fields as well as the yard and the daylily beds are mowed on a rotating schedule. Daylilies are split and new planting beds are built. The barns get a good cleaning before being set up as equipment space and sales areas. Late May is filled with fun, music and magic with our Fairies on the Farm weekend.
Please leave your pets at home. We love animals here at Heritage Farms but for the safety of our customers and staff please do not bring your pets.


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Map of College The University of Chicago Description Private Apply Requirements History The first classes were held on October 1,1892 Surrounding 211 acres Maroon White Mascot Phoenix Chicago, Illinois William Rainey Harper became the University's first president on July 1, 1891 Harper died in
1906. Application fee: The $75 non-refund application fee may be paid by credit card or money order. UChicago requires an ACT or SAT Reasoning score, and they use your best results if you send multiple test scores to them. The University of Chicago Supplement requires one extended essay of your choice from a list of five, and one short essay on why you would like to attend the University of Chicago. Academics The College offers more than 50 areas of specialized study, complementing the breadth of the Core curriculum with an opportunity to delve into a particular area. Majors Art History Biological Chemistry Music Computer Science
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The Advoc8te is still alive and kicking, even if I am not blogging as much. As you should know by now I took a new job recently and I am working really hard. That means that I have to go to bed at a reasonable hour -- no more 2am editorials!
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Ryan Gajudo Macasero '12 (Photo: Josh Patterson/New America Media)
CSUEB grad wins ethnic media journalism award
- April 3, 2013 5:00am
Ryan Gajudo Macasero, BA, international studies, ('12), was chosen as one of four Emerging Youth Voice awardees by the New America Media for his personal essay on overcoming depression as a young Asian American. The story originally appeared in The FilAm.
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Today, I received a text message from my older brother. It said, "Ah... I want you". I hope to god it was intended for someone else. FML
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Open reaction systems which are of the quasi-thermodynamic type exhibit a certain dynamic regularity, e.g. asymptotic stability for steady states in which all concentrations are positive (Horn & Jackson 1972). In this paper it is shown that with certain open reaction systems there may be associated a graph, called the complex graph, such that the non-existence of 'even cycles' and 'odd dumbbells' in that graph ensures quasi-thermodynamic behaviour for the underlying open system. Furthermore, an introduction is given to the combinatorial analysis of open reaction systems.
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Tube sounding distortion. What about an injection of vitamins in your tone ? The TONIX is a natural, fat sounding pedal, ideal for bluesy stuff and chordal playing, when you want all the notes to be heard. Only three knobs, yet an infinity of sounds. It is simple but has no limitations.
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According to Lee Dickson this guitar was kept as a rehearsal guitar, it is the only Strat made for Clapton with these Texas Special pickups. Clapton commented that he prefers Lace Sensor pickups. Lee Dickson and Larry Brooks of Fender dreamt up the name Whitey for this guitar because it looked like a traditional Strat with visible pole pieces in keeping with Clapton's other named guitars, Blackie and Brownie. Clapton apparently didn't use this name himself.more
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Three Finger VS Chaves Wrong Turn 3 Left For Dead MP3
The 3rd Movie - HillBilly VS Chavez.
Three Finger Wrong Turn Laugh Extended MP3
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Or more like dramatic Three-Finger who screams and nags at his big brother for disturbing his master work XD One of my favourite scenes of this awesome ...
Wrong Turn 5 Funny finger scene MP3
Funniest horror scene of all time...
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A Tribute to "Three Finger" from Wrong Turn (Horror/Slasher). Including: - Wrong Turn 3 - Wrong Turn 5 - Wrong Turn 6 Music: Woodkid - Run boy run. Copyright ...
Wrong Turn 3: Left for Dead MP3
It's hunting season in the deep back woods for Three Finger and his family of hideously deformed inbred cannibal hillbillies! The fight for survival is futile and ...
Five Finger Death Punch - Wrong Side Of Heaven MP3
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Wrong Side Of Heaven
Five Finger Death PunchI spoke to God today, and She said that She's ashamed What have I become? What have I done? I spoke to the devil today, and he swears he's not to blame And I understood, 'cause I feel the same[...]
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A group of college students gets lost in a storm during their snowmobiling trip, and takes shelter in an abandoned sanatorium, which is the home of deformed ...
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Made by me -333 Selena Gomez & The Scene - A Year Without Rain - 3.
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A cruel mutated cannibal kills a man and a woman.
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Wrong Turn 2 Dead End is a 2007 American film directed by Joe Lynch and starring Erica Leerhsen, Henry Rollins and Texas Battle. It stars Erica Leerhsen as ...
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Page Object?WTF?
Project with standard selectors
Using Site Prism
Page Object
Ruslan Naumenko
Software Developer in Test
skype: naumerus
Page object its good pattern of web automation.
Make Your code better.
Don't use 'sleep' because every time that U used 'sleep' in world dies one unicorn and cutie kitty. Lets save population of unicorns ) This panda will watch on You.
Step definition file for profile page
Structure of project & feature file for genres
And main painful thing - selectors
Installation of SitePrism:
gem install site_prism
Using SitePrism with Cucumber:
require 'capybara'
require 'capybara/cucumber'
require 'selenium-webdriver'
require 'site_prism'
Page Object file structure and simplest element and sections
How its could applied on practice in step definition file
Working with URL of pages
Interacting with IFrames
Anonymous section
Create a class containing methods that return instances of the pages
Simplest method that we could call by using SitePrism
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This hotel is located couple blocks from the capitol hill and union station. Clean and comfortable hotel. The experience was soured tremendously when my bags were stolen out of my car in while it was parked int he garage (valet). There was no damage to my car - so the valet attendant must have either left it unlocked or one of the attendants participated in the theft. The hotel took no responsibility because they subcontract valet parking. The valet parking operation never returned my calls and gave me the runaround every time I called. I strongly suggest not to stay at this hotel.
- Official Description (provided by the hotel):
- A sophisticated, independent hotel located in downtown Washington DC, the Washington Court Hotel offers the ultimate in modern design and hospitality. Boasting a central location near Union Station and Capitol Hill, the Washington Court Hotel is a 265 room distinguished downtown destination for both business, leisure travelers and DC's elite. Guests at the Washington Court Hotel can explore the rich history of Washington DC just by stepping outside our front doors. Situated adjacent to Union Station, our 16-story property is located in the heart of the distinctive Capitol Hill neighborhood providing access to the National Mall, renowned monuments, free museums and the U.S. Capitol. Luxuriate in world-class surroundings, complete with Italian marble bathrooms, well-lit vanities, and haute technology including flat-screen TVs and high-speed wireless Internet access. As a truly exceptional destination among downtown Washington DC hotels, Washington Court Hotel is an inspired choice for discerning visitors of Capitol Hill. At Washington Court Hotel, your experience plays center stage to everything we do. A family-friendly adventure, business outing or solo excursion – our helpful staff is at your disposal to set the tone for an experience that is unrivaled on "The Hill". ... more less
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- Also Known As:
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- Washington Court Hotel


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Ludwig-Maximilians-University Munich, Medizinische Klinik und Poliklinik IV, Schillerstrasse 42, Munich D-80336, Germany
Ludwig-Maximilians-University Munich, Department of Biology II, Grosshaderner Strasse 2, Martinsried D-82152, Germany
Ludwig-Maximilians-University Munich, Department of Internal Medicine II, Marchioninistrasse 15, Munich D-81377, Germany
Apceth GmbH & Co., KG, Max-Lebsche-Platz 30, Munich D-81377, Germany
Mesenchymal stem cell (MSC) homing and integration into tumors are under evaluation for clinical application. This approach requires the identification of conditions for optimal tumor invasion. We describe a tool for the in vitro comparison of parameters influencing invasion. Human MSC added to experimental tumor spheroids variably migrates toward the center of the structure. To determine MSC distribution inside the three-dimensional specimen, spatial analysis was performed using selective plane illumination microscopy. A standardized method to quantify and compare the invasion potential of variably treated MSC into experimental tumor environments allows efficient screening for optimizing conditions.
Mesenchymal stem cells (MSCs) are actively recruited to tumor microenvironments where they can promote tumor growth. Because tumors are seen by the body as chronic wounds, they attract MSC in an attempt to effect repair.1,2 This property has been exploited to generate engineered MSC (eMSC) that acts as cellular vehicles carrying therapy genes deep into tumor environments. An early version of the eMSC is being evaluated in clinical trials for the treatment of gastroenterological malignancies.3,4 Studies have shown that different preparations of eMSC can show variability in their ability to invade tumors. This has driven the search for methods that would allow for a systematic evaluation of the effects of different variables on the efficiency of eMSC to invade tumors.
In vitro derived tumor spheroids have been shown to recapitulate features of their solid tumor counterparts, specifically, aspects of intervascular tumor microregions.5,6 They provide insight into tumor composition, growth, and physiology offering an easily manageable tool for cancer studies, including a platform for evaluating the ability of eMSC to invade tumor environments.
We developed an in vitro tool for quantification of MSC invasion into tumor spheroids. It uses selective plane illumination microscopy (SPIM) which allows three-dimensional (3-D) image acquisition and rapid-robust screening of parameters impacting MSC infiltration into tumor environments.
Human hepatocellular carcinoma-cell (HUH7) spheroids were used as tumor models. Primary human MSCs extracted from bone marrow (provided by apceth GmbH, Munich, Germany) were stained with 1 μM CellTracker Green 5-chloromethylfluorescein diacetate (CMFDA, Molecular Probes, Thermo Fischer Scientific, Waltham, Massachusetts) as detailed by the manufacturer.
The HUH7 cell line was used to generate tumor spheroids based in part on previously established protocols.7 Briefly, cell attachment was inhibited by coating culture dishes with the hydrogel poly(2-hydroxyethyl methacrylate) (polyHEMA, Sigma-Aldrich, St. Louis, Missouri). The cells were cultured under normal cell culture conditions (37°C, 5% CO2) in a medium consisting of Dulbecco's modified eagle's medium with GlutaMAX (Gibco, Thermo Fischer Scientific, Waltham, Massachusetts) containing 10% fetal calf serum (Merck, Darmstadt, Germany) and 1% penicillin/streptomycin (General Electric, Fairfield, Connecticut) in the treated dishes. After 5 to 7 days, spheroids between 200 and 300 μm in diameter were used for the invasion assay.
A standardized invasion protocol was established to allow comparable results. To this end, 2.5×104 CMFDA stained MSCs (determined using a Neubauer counting chamber) were added to a 1.5-ml reaction tube containing a single spheroid in a total volume of 50 μl cell culture medium. The tube was then rotated horizontally at 36 rpm for 2 h at room temperature, thus ensuring an even distribution and attachment of MSC to the spheroid surface. MSCs that had not attached were washed away using three times 1-ml cell culture medium. Each spheroid-MSC preparation was incubated in a volume of 50 μl cell culture medium for a period of 24 h in a polyHEMA coated well of a 96-well plate under normal cell culture conditions. Fixation using 4% formalin (Sigma-Aldrich, St. Louis, Missouri) for 2 h at room temperature and storage in 50% glycerol (Roth, Karlsruhe, Germany) and 0.2% propyl gallate (Sigma-Aldrich, St. Louis, Missouri) in phosphate-buffered saline at 4°C to avoid bleaching allows large numbers of spheroids to be tested in parallel.
Quantification of invasion depth requires localization of individual MSC. Widefield microscopy of whole spheroids did not provide usable information in this 3-D context. Cryosectioning allows analysis in the third dimension, but requires extensive sample preparation. To this end, "optical sectioning" via 3-D imaging techniques was used (see Fig. 1, Videos 1 and 2). As compared to two-photon laser scanning microscopy, light sheet technology allows rapid image acquisition, and therefore, lends itself well to this screening approach.
Methods to image mesenchymal stem cell (MSC) invasion into tumor spheroids. Human MSCs were stained with 10 μM (a–c) or 1 μM (d) CellTracker Green CMFDA (Molecular probes®) prior to invasion. The cells were incubated for 24 h with HUH7 spheroids as described. Whole spheroids were fixed with 4% formalin and, in (a–c), DAPI stained. (a) and (b) show two-dimensional (2-D) images: widefield microscopy of (a) whole spheroid, and (b), a cryosection, signals of 365 nm (blue) and 470 nm (green) excitation were merged. (c) and (d) show single optical slices out of three-dimensional (3-D) stacks imaged with (c) two-photon microscopy with an excitation wavelength of 760 nm and laser power of 120 mW, signals from emission channels below (blue) and above (green) 500 nm were merged, and (d) selective plane illumination microscopy (SPIM) OpenSPIM using a 488 nm laser. Video 1: Visualization of passage three MSC invasion into tumor spheroids. Deconvolved SPIM data, animation done with the help of AMIRA Software (FEI, Dawson, Oregon). (Video 1, MPEG, 17.5 MB) [URL: http://dx.doi.org/10.1117/1.JBO.20.4.040501.1]. Video 2: Visualization of passage seven MSC invasion into tumor spheroids. Deconvolved SPIM data, animation done with the help of AMIRA Software (FEI, Dawson, Oregon). (Videoo 2, MPEG, 17.4 MB) [URL: http://dx.doi.org/10.1117/1.JBO.20.4.040501.2].
Light-sheet or SPIM8 is based on the principle of laser excitation confined to the focal plane. Specimens are illuminated by a laser light sheet perpendicular to the detection axis thereby depleting the out of focus signal to a minimum. Samples are rotated to acquire stacks from several angles prior to computation of 3-D reconstructions.
The spheroids were embedded in 1% 2-hydroxyethylagarose (Type VII, low gelling temperature, Sigma Aldrich, Missouri) mixed with fluorescent microspheres (F-XC 50 Estapor, Merck, Darmstadt, Germany; 1∶4000 dilution) and aspirated into a glass capillary. Mounted on an OpenSPIM set-up, they were imaged with the solidified agarose hanging directly in phosphate-buffered saline in front of the detection lens. The SPIM application is available via open access hardware and open source software9,10 (detailed instructions of SPIM assembly, sample mounting, and imaging11).
SPIM imaging was performed using a 488 nm laser (Cube, Coherent, Santa Clara, California; 2 mW laser power, 120 ms exposure time) and an sCMOS camera (Orca-flash 4.0 V2, Hamamatsu, Hamamatsu City, Japan) from five different angles equally spaced over 360 deg, controlled via the Fiji μManager plugin.12,13 The subsequent bead registration and fusion of images were performed using open source software on Fiji.14,15 To minimize data volume, images were acquired with a 2×2 binning, and for subsequent analysis down sampled by a factor of 4. For visualization, images were deconvolved without down-sampling (Videos 1 and 2).16 The resolution acquired with a 20× water immersion objective (NA 0.5) and a lightsheet thickness of 15 μm full width at half maximum is 540 nm±112 in x, 599 nm±128 in y, and 3908 nm±954 in z for raw image stacks, 1159 nm±185 in x, 862 nm±99 in y, and 1933 nm±1089 in z for fused data, and 378 nm±127 in x, 355 nm±67 in y, and 978 nm±58 in z after deconvolution.17
The CMFDA signals were segmented using the Fiji 3-D object counter plugin.18 The fused 32-bit grayscale images (16-bit can be alternatively chosen with the updated version of the plugin) were converted into 8-bit by linearly scaling the display range from 0 to 9000 pixel values from the original. Hence, a segmentation threshold was typically set to 70 pixel values and the minimum size filter was set to 200 voxels (to exclude fiducial beads and artifacts).
The autofluorescent spheroid was segmented in the same manner with a threshold between 14 and 18 pixel values (tested in advance) and a minimum size of 105 5 voxels. Invasion depths were quantified by the measurement of distances from the center of each MSC to the border of the spheroid using the Fiji 3-D manager plugin.19 The image processing and analysis steps were conducted in a semi-automated fashion via macros developed with ImageJ.20
Cellular activation on plastic and components of standard culture media including factors released by MSC themselves have been shown to influence the migratory behavior of (stem) cells.21 As a proof of concept for the assay, the effect of expanded culture on the passaging of MSC isolated from one donor was tested. The results suggested an enhanced ability of the cells to invade the spheroid after expanded passaging. A significant increase of invasion depths was found between passages three and five. Longer passaging of up to passage seven showed no further increase (see Fig. 2). The results support the contention that limited expansion of early isolates may help to shape their tumor tropism.
Quantification of MSC invasion into tumor spheroids. The migratory distances of individual MSC from the spheroid surface are plotted. Primary MSCs from passages three, five, and seven were analyzed following an invasion time of 24 h. Three spheroids per condition were imaged, total numbers of MSC found (n) are indicated above. n.s.: not significant, *P<0.01 (Kruskal–Wallis test) (see also Fig. 1, Videos 1 and 2).
To optimize MSC-based tumor therapies, one important parameter is the migratory efficiency of MSC into tumor tissue. The in vitro invasion assay described here provides a straightforward method for the analysis of MSC lots and pretreatment regimens on the ability of the cells to invade experimental tumors. Importantly, this approach should also be directly applicable for the investigation of other tumor infiltrating cell types, such as leukocytes, which are also under development for advanced tumor therapy.
We thank David Hoerl, LMU, Munich, Germany, for determining the resolution of the system. Funding was provided by the German Research Foundation-DFG "THYROID TRANS ACT" (SPP 1629) to C. S. and P. J. N. R. H. works for apceth GmbH, a firm conducting clinical trials of MSC-based therapy. No other potential conflict of interest exists. Work performed as partial fulfillment in the doctoral thesis of S. R. within the LMU Medical Faculty.
Svenja Rühland ; Alexandra Wechselberger ; Christine Spitzweg ; Ralf Huss ; Peter J. Nelson, et al."Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy", J. Biomed. Opt. 20(4), 040501 (Apr 03, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.4.040501
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Debbie Macomber's Trading Christmas is a made-for-TV Christmas movie based on the novel of the same name by Debbie Macomber. It premiered on the Hallmark Channel on November 26, 2011.
The movie was released on DVD by Gaiam Vivendi Entertainment on October 30, 2012.
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ate at a restaraunt called kushiyaki dan yesterday by chance. anyways turns out they serve oden everyday. it was very good. also had nato with kimchi which i never had before. the chef is japanese, and for the most part it is traditional japanese yakatori and oden. they do have some japanese korean mixed items though.
4001 W. Olympic Blvd., Los Angeles


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Search Results Type: Posts; User: CRAZY33
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I start Sept 2012 class and they accepted my public communication class in place of public speaking.Good Luck!Sep 2, '12
- Forum: Nursing Online Distance Learning Tips
Hello future RN's......I like the way THAT sounds!:yeah: I also start the program Sep17th. I am freaking out a little for lack of "a plan". I received my letter with book list and calander of...Sep 1, '12
- Forum: LPN to RN Nursing Student


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The internet is still slandering Rich Homie Quan for egregiously screwing up Notorious B.I.G.'s lyrics at VH1's Hip-Hop Honors show Monday night.
While VH1 probably wants to take back that moment, it did get Teyana Taylor saluting Lil' Kim right. And Iman Shumpert made sure to let the world know about it.
The Cleveland Cavaliers' champion took to his Instagram to gloat about his baby mama nailing her portrayal of the Queen Bee at Hip-Hop Honors.
She did kill it, didn't she?
Take a look at Shump's heartfelt sentiments.
Go ahead, Shump! Talk that talk!
Hey, he's speaking the truth. Teyana definitely bodied that performance.
BET Sports News — Get the latest news and information about African-Americans in sports, including weekly recaps, celebrity news and photos of your favorite Black athletes.
(Photo: Michael Loccisano/Getty Images for VH1)


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oh yes. i did buy oxfords. and guess what, i actually love them! i'm wearing them right now. they are actually very lady like and low cut. excellent ankle cleavage dont worry.
Are you really going to purchase oxford shoes?? Eeeek! ; ) Haha! I love it…can't wait to hear the reactions…hilarious!


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Paris Hilton has no friends, throws weird parties
Is she trying to make love to that cake?
Ugh, clicked "post" by accident—is she trying to make love to that cake? And boy, that's a way to be sure you're the center of attention for your birthday party: be the only one there. Why didn't I think of that? Oh wait, I was too busy not doing cocaine and suckin' dicks like a semen-powered Hoover.
this is just too bizarre. a pack of fucking MIDGETS leading around goats??!?!?!?! truth is stranger than fiction indeed.
Paris the Dancing Clown Slut
Now available for childrens partys.
Pony costs extra.
Reportedly she DID manage to blow all the candles. Unfortunately all the goats were named 'candles'.
Go back and take a good look at those Man Hands. She's giving Pink a run for her money with those giant meat-paddles.
I like how the guy in the next to last picture is all 'her vagina is THIS deep!'
Um, is that a star and hand prints on her cake? Interesting. Midgets and goats, I expected that.
Apparently the goats and midgets were in one of those 'Paris Exposed' vids, but PETA started asking questions.
I was there.
I had a blast.
#10 I wouldn't admit that. That's actually sad.
geez, paris hilton sucks.
That poor cake.
At least it has candles to defend itself with.
Now I feel sorry for Iran.
Fuckin' people in the audience…idiots….they oughta be gassed.
I bet Matt Leinart didn't even show up.
Pack of goats? If you didn't think she was Satan's spawn before.. well here's your proof.
What??? You don't know who he is??? Ever heard of USC, only just the greatestest ever college football program ever (yes, including Orenthal James)? PUSSIES!
hey, I already "reported" on the midget/goat/monkey thing in the previous Paris article. Seriously, I'm now reporting on things before The Superficial?! That seems kinda sad given my lack of resources for celebrity gossip.
This just in… Paris Hilton hospitalized for 3rd degree wax burns to her lips. Sources say she got confused when they told her to "blow out the candles".
— Jim
#16 weren't you a little premature in blasting people about not knowing who Leinart is before anyone even said they didn't know who he is? Kind of like your premature claim that USC is the greatest college football program ever! But that's a debate for a different website!
"More of Paris Hilton thinking she's the shit at her birthday party after the jump."
Is is the shit… the one you can't flush down the toilet no matter how hard you plunge.
I wonder how much it cost to make all the homeless people looked groomed and pretend to be fans?


text:
How to Buy
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I'm 22 years old. I often loose and gain weight; but most often loose. How can I get rid of fat on my neck? I have friends that are bigger and dont have a fat neck.
How to Get Rid of Neck Fat?
Doctor Answers 24
Fat on the neck and chin
good morning thank you for the inquiry
The answer is it may simply be anatomy and genetics. Though some people may be actually somewhat large in stature or overall size their necks may be longer than others in their jaw may project in the lower hanging than others giving them a more defined look despite being a larger person. some people just have a low hanging hyoid bone that is low and anterior making it difficult to create a well defined jaw, chin and neck. Again, if you don't have a strong projected chin and jaw this works against you as well. In these instances no amount of exercise, weight loss, etc. will change the way that looks significantly.
given your age laser assisted liposuction (SlimLipo) and possibly a chin implant maybe your best bet.
Learn more: http://chaselaymd.com/SlimLipo_Jaw_Neck_Lift.php
Getting rid of neck fat
No non-surgical procedures work other than weight loss. The 3 surgeries one can have are liposuction, neck lifts, and facelifts. At 22, I would hope you still have good enough skin elasticity that liposuction alone would work for you.
Learn more: http://www.randcosmeticsurgery.com
Fat in the right places
This is very stubborn fat. It is not you. It is your parents to blame. This is genetic and stubborn fat. The best way to remove it is micro liposuction and sculpting of the neck and jaw. The thing now to consider is if you can use this fat to augment other areas such as the chin, cheeks, cheek bones or periocular area.
Hope this helps.
Best of luck,
You might also like...
Neck Fat, Double Chin
Most young people with excess neck fat will do well with a liposuction procedure. This can usually be performed through a small incision below the chin which typically leaves a barely noticeable scar.
Learn more: http://drcarniol.com/mini-facelift-non-surgical-facelift-facial-tightening/
Multiple Option for Correcting Your Double Chin
Treatment for Neck Fat
Newer methods to remove submental fat include Laser Lipolysis and Kybella. Laser lipolysis melts fat and stimulates skin contraction and may be combined with traditional liposuction or is a stand alone procedure. Kybella is a new FDA approved medication that is injected into the submental fat to melt it over time. These injections are repeated.
Learn more: http://www.faceologymd.com/neck-procedures-newport-beach/
Neck Fat: Alternatives
A new injectable alternative will be available perhaps as soon as Fall 2015 pending FDA approval: ATX-101. This alternative will be an office infection which will dissolve fat in the neck/submental area. Again, patients with good skin elasticity will probably benefit most from this noninvasive treatment.
Learn more: http://cosmeticsurgerytimes.modernmedicine.com/cosmetic-surgery-times/news/atx-101-receives-unanimous-support-fda-approval
How to get rid of neck fat
There are 2 types of fatty deposits in the neck, and these are sub-platysmal, and sub-mental. The sub-mental fat deposits can be removed with traditional liposuction, however the sub-platysmal fat must be removed with a neck lift. A platysma-plasty is also performed at same time as a neck lift to give further jawline definition. For many examples, please see the link below to our neck lift photo gallery
Learn more: http://www.seattlefacial.com/photogallery/neckliftandlipo_photos.html
Neck Fat
Weight loss is the only non surgical option. After your weight is stable, chin liposuction is a good option.
Learn more: http://www.capefearaesthetics.com/details/plastic+surgery-19/
Treatment Of Fat Neck?
Thank you for the question.
Unfortunately, it is not possible to give you good advice without direct examination. Generally, a good candidate for liposuction of the neck and chin area will be a relatively young patient with isolated "excess" adipose tissue with limited redundant/loose skin. The quality of overlying skin elasticity is of utmost importance. In my practice, the majority of patients I see who request the procedure are not good candidates.
You will find many doctors will offer their services to you; I would suggest consultation with well experienced board-certified plastic surgeons.
I hope this helps.
Learn more: http://www.poustiplasticsurgery.com/Procedures/procedure_liposuction.htm
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.