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best umbrella etter recommendation than that! very comfortable and no problem with grip at all.Although with Adidas i find i have to go up a size I have had quite a few different golf shoes over the years I find adidas shoes very comfortable bought I half size larger which gives a very good fit very comfortable --even with my wide feet !! So far I've played 2 rounds in them -- so far everything is good!!Delivered on time Nice looking fit well very comfortable this is my hubb Oslo plained about my shoes!!!!!! These have solved the problem, and they smile at me at 5.30 in the morning before I go to work. They are O.K. they help with the pong. my bag smells better for them. not great but worth a try. great at keeping the smells at bay As soon as it was released it was obvious that 'Splinter' was going to be overlooked, which is a real shame given that it is, quite frankly, amazing.It is true to say that the more memorable songs do appear at the start of the album, but it is unfair to say that it tails garmin zumo 660 best price ever bought foot wear online before so didn't think it should be a problem with 7 as a guide. The shoes are everything you expect from Timberland, but these seemed bigger than the stated 7 size and wider too. Returned to Amazon without any issues. And cannot fault the customer service I received from Amazon. 5 Stars to them. Having spent £100 i expected more than 12 months of wear out of these things. The sole on one shoe has opened up letting in water.The outer leather surface in the region where the foot bends has flaked away.Terrible performance from Timberland after many years of using a few of their boot designs, I'm disappointed in their casual footware. Won't consider another purchase again. Bought these as replacements as my old Timberlands, which were wearing out after a year of hard work! Very comfortable and although they are not the most stylish or the cheapest work shoe. They last and provide best v nono pro reviews superfast delivery.. just put an order in for the pinks.. Came on time, beautiful heels! Amazing, beautiful and probably the most comfortable shoes I've worn. Lots of comments asking where did I get my seriously sexy shoes ;) I should really take a size six but opted for 6.5 because I always have problems with the width fitting on my right foot and get a lot of pain if the shoe doesn't fit. So it flops off my foot a bit but it isn't painful It is really helpful that there was a half size in this shoe. The shoe itself is very comfortable to walk in and has a sort o.
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Great shoes, very comfortable and well-made. Just a bit small in the toe area; I'm somewhere between size 8 and 8.5 and in this case size 8 is certainly too small.
Very comfortable, wearing them now. And writing fifteen words to be able to submit this review. Umbrella, fish, chair, table.
First Bought a pair of these by chance in a camping shop a couple of years ago and wore them everywhere.This is the second pair. Most comfortable slip on shoe I have ever had, and requires almost zero maintenance.
I was lucky enough to find a pair of these shoes in my size in a Debenham's sale for £18 about 3 years ago. As soon as I tried them on I leet. I still think this was the right decision as a 5 would have been too small.They are gorgeous comfy shoes. I love Fly and have some of their boots too. I am a convert and think I will only be buying Fly footwear now!
I chose the grey/ black patent size 8 and they looked gorgeous but they were too tight and pinched at my toes when I tried them on, so unfortunately I had to return them. I usually have to buy shoes between a UK size 8 or 9 and have a pair of Yedi Perf size 8 which fit perfectly.
I normally take a size 6. In Fly London, I usually take a size 7. I ordered 2 pairs: Yeo perf sandals in size 7 (too tight, but now stretched to fit) and these ones in size 6 (Massive - like boats) Sadly, I have to return these really beautiful and well-made shoes. At this price, I would have been over the moon if they had fitted.When will Fly London get to grips with this very poor sizing issue? They are such gorgeous shoes (I own 10 pairs) but it is pot luck whether they will come up anywhere near your usual size.
Bou Babyliss Big Hair would recommend to everyone
These shoes are strong as I have a testing lifestyle for my shoes and they were exactly what I wanted. The only thing that was wrong was I selected a size a little too small.
for £17 these are the best shoes I've ever bought. The don't fit me sadly but they are nice quality, just perfect
for a budget show very good, they don't look amazing but for the price very good, and most importantly very comfurtable, i would buy some more for casual wear.arrival and delivery packaging Hollister outlet size and i am very satisfied with the sizing. it's not too big, with just enough allowance on the tips of my toes and roomy enough in terms of width for those with wide feet like me (those with narrow feet might find it rather 'big' though). i would say, it is true to size - so order your usual shoe size. very comfortable! the only downside for me is the color. i picked 'sherwood green' as it seemed more like 'olive' on the photos but when they arrived they look much greener and more like the 'cactus' ones. i'm keeping them nonetheless since the fit is great and i can still wear them with white, blue, gray, black and camel jeans. :) perfect for summer and walking around in europe - highly recommended!
These were bought for my daughters birthday as a gift. She loves them. They fit well and seem true to size.
Wouldn't hesitate to recommend these. Colour and fit exactly as expected. Delivery was very quick, arrived the very next day. So comfortable and they look great too. Really versatile. Haven't seen them at a better price than what I paid either.
I saw.
best kiteboard good shoes, as Merrells always are. If they're anything like the two previous pairs I've had, they should last 3 or 4 years before they need to be replaced.
Had not ordered shoes online before - but after my local store never having my size in stock, decided to try it.Was very pleased - shoes arrived the following day and were so comfy from day one - didn't need wearing in - nota blister or rub in sight.Really pleased - as I needed a shoe that could be worn for dog walking several miles every day - and these certainly meetthat mark.Would recommend them anytime.
This is my fifth pair of Merrell Siren Sport shoes. I wear them all the time, they are so practical and comfortable. The Goretex waterproofing means they are perfect all weather, work and walking shoes. Be aware that the sizing is smaller than other shoes, I take a size 6 oo the heel part has a tendency to flick like an elastic band on removal from the shoe (mind your wrist). Otherwise great and does exactly what you expect.
I thought there was a catch because these were so much more affordable than other cedar shoe trees - but these are perfect. The middle bit with the spring makes it really easy to put them in and take them out...and these are so easy to store as well. They're an easy investment to make sure my nice shoes dont get creased as quickly and retain their shape. Don't let the odd looking design fool you - these are fantastic.
Kindda of a must these shoe trees and the ones reviewed now appear to be the cheapest on amazon.They keep the shoes stretched. The more expensive shoe trees also stretch the shoes by pushing the sides with springs. My question is whether you actually need the sides strecthed, as Hollister uk hey are real leather - that is a plus for me, as I don't want to wear leather.
Loved the shoes. I need an E fit and that wasnt made clear when ordering. I was sent a D fit.
Too small/tight
Label said 'Wider fitting available' but this was not an option when purchasing.
great value i wear them for work and am on my feet for 12 hours plus. Very comfortable.
tighter than expected but very comfortable and soon 'wore in'
comfortable on, have played at badminton and went out walking
Lovely calendar and great value for money. Prompt delivery & service.
Ex purchase.......HANX
?? many thanks ??
I purchased these as an alternative to my beloved Converse as they seemed heavier and would hopefully more hard-wearing as a result. I loved the colour palette available and chose the bright and breezy 'Caribbean Blue' (isn't that the title of an Enya album?) Size wise, I'm a size 9/EU 43 it's often difficult to find funky shoes; fit wise, being Italian made they do come up a little small but fitted me - I'd maybe suggest going up half a s Babyliss UK ed my feet were in previous shoes). First run was 12k and no rubbing, blisters, etc. The shoe although very cushioned is soft and light. Honestly recommend. Oh and £60 is great price. Happy running!I can also mention that Saucony 6 in size 6 felt small and tight. Brooks 14 in size 6 beautifully comfortable.
I've brought Brooks running shoes since 2006 till earlier this year when I changed to Asics but due to niggling strains gone back to these shoes. Always reliable stability even though I have a neutral running style
I like the shoe overall. It feels solidly constructed and does a good job at supporting the inside of my arch. It seems to be relieving some ankle pain I was having. I'm hyp.


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2004-01-19 at 9:39:00 am #4724
KONICA MINOLTA BUSINESS SOLUTIONS U.S.A. NAMES STEPHEN T. JONES EXECUTIVE VICE PRESIDENT U.S. DEALER SALES Konica Minolta Business Solutions U.S.A., Inc. on Friday announced that Stephen (Steve) T. Jones has been named executive vice president, Konica Minolta U.S. dealer sales. Jones, who previously served as senior vice president, Konica U.S. dealer sales, will assume his new position immediately, and will continue to report directly to Yasuo Matsumoto, president and CEO.
In his new role, Jones will be responsible for overseeing the smooth and effective transition of Konica Minolta's dealers — a group of approximately 700 dealers that were brought together as a result of the merger of Konica Business Technologies U.S.A, Inc. and Minolta Corporation's Business Products Group in October 2003 — into an integrated channel. Jones' primary goals are to form a united dealer channel, ensuring that each of the legacy channels receives proper representation and support; and achieving increased dealer revenue through the sale of the company's production print, color and solutions technology. In addition, he will be helping to streamline and strengthen the sales, service and administrative processes that support the dealer channel; and educate the dealers regarding current and future Konica Minolta products and solutions, as well as company direction.
Jones joined Konica three years ago to develop and implement a color product strategy and surrounding sales effort. In April 2003, Jones succeeded Richard (Dick) Peterson as senior vice president, U.S. dealer sales, and was responsible for the continued growth of Konica's dealer community. Prior to Konica, Jones was vice president, sales, for Sharp Electronics Corporation, where he led the U.S. sales efforts for the company's color products along with its commercial copier and fax technology lines. In addition to his experience at Sharp, Jones was director of color operations worldwide at Xerox, handling the design and management of color and digital products sales and marketing initiatives.
"During the past year, Steve has successfully managed Konica's dealer sales operations, achieving both sales and growth objectives. Steve has proven that he has the knowledge and skills to solidify the Konica Minolta dealer organization and turn it into the strongest dealer channel in the industry," said Yasuo Matsumoto, president and CEO, Konica Minolta Business Solutions U.S.A., Inc.
"I am looking forward to building the business framework and team to compete effectively with the industry leaders — Canon and Xerox," said Steve Jones, executive vice president, Konica Minolta U.S. dealer sales. "It is my goal to surpass the competition and become the premier provider of imaging products and services. This will be achieved by providing the dealer channel with superior tools, training, marketing support and management resources."

subset: commoncrawl


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The Big Easy. Space City. The Rock & Roll Capital of the World. And...Burlington, VT?
That's right -- the Queen City of New England now has its own Maps Rocks Glass, too. In fact, one of our guys just got married there not too long ago, so we outfitted the reception with some brand spankin' new Burlington Maps Glassware to kick things off right. Let's just say there wasn't a dry eye -- or glass -- in the place.
So check it out along with our other new offerings in our rapidly growing Street Maps Glassware collection.
To your health, to your neighborhood!


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Prudence Mapstone put out a call for freeform submissions January 2015. What a beautiful and happy way to start the year. The call is for freeform enthusiasts around the world to contribute something flowery in happy colours. For more information, check this link:
Flower Power Exhibit
This is my submission. :)
Have a wonderful and blessed New Year. XOXOXO!
UPDATE! The freeform piece made it to it's destination of Queensland, Australia. From there it will be joined with several freeform submissions from all over the world. If you want to read about the exhibition, see the freeform pieces and countries they come from, here is the link to this blog 50 Years of Flower Power
Sunday, January 25, 2015
Flower Power Exhibition Australian Challenge
Posted by lilquiz at 2:55 PM
2 comments:
Your work is so fantastic and sophisticated! Thanks for the link to the exhibition! I like flowers. France has inspired me to live with its gorgeous streets and flower beds. And, of course, some gorgeous men nearby:) Merry Christmas!ReplyDelete
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1. Choose a finance option
0% Finance couldn't be easier to arrange, enabling you to spread the cost of your new bike or equipment and pay nothing in interest. Simply use the handy calculator to the right to get an indication of your repayments. At checkout, fill in the online application form and we'll sort the rest.
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True freedom can only be experienced when you're willing to try something completely new - and the Nutrail Hybrid is the perfect tool for the job. With 4.0 inch tyres and powerful Bosch CX drive it quite simply knows no limits, rolling on with nonchalent ease whether you're out in the sticks or in the thick of the urban jungle. This CUBE masterpiece - with an aluminium frame designed specifically around the needs of fat tyres and the Bosch drive system - contains all of our know-how and fastidious attention to detail. It's these high quality features that have produced a Fat e-bike that blends the fun factor with genuine everyday practicality.
- Specification
Gender Mens Discipline Electric MTB Frame Aluminium Superlite, Double Butted, Tapered, Disc Only, X12 Fork Rock Shox Bluto RL Solo Air, 100mm Headset FSA 1.5E ZS, Tapered, Top Zero-Stack 1 1/8" (OD 44mm), Bottom Zero-Stack 1 1/2" (OD 56mm) Stem CUBE Performance Stem Pro, 31.8mm Handlebars CUBE Rise Trail Bar Pro, 740mm Grips CUBE Race Grip, 1-Clamp Brakes Magura MT5, Hydr. Disc Brake (180/180) Rear Derailleur Shimano XT RD-M8000-GS, 11-Speed Shifters Shimano XT SL-M8000, 11-Speed Chainset CUBE Fat Crank, 16T Pedals No - Click here to see our pedals » Wheelset Answer Fat Atac 80SL HR 12x177mm VR 15x150mm Front Tyre F+R: Schwalbe Jumbo Jim, Kevlar, 4.0" Rear Tyre F+R: Schwalbe Jumbo Jim, Kevlar, 4.0" Saddle Selle Italia SC1 Seat Post CUBE 120mm Adjustable Seatpost, Handlebar Lever, Internal Cable Routing, 30.9x420mm Extras ENGINE: Bosch Drive Unit Performance CX (75Nm) Cruise (250Watt) Extras BATTERY: Bosch PowerPack 500
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Review "Cube Nutrail Hybrid 500 : Black/Green 2017"
*Price based on 0% deposit and 36 monthly installments | ** On orders over £50.00. Due to restrictions from the manufacturer we are unable to ship Trek, Whyte, Cervelo, Focus and Wilier bikes. Please contact us for more information and advice. Excludes Highlands and Isles which will be quoted separately. We do our very best to ensure product accuracy but from time to time specification and product images may be subject to change. Please contact us for any product queries you may have.
PLEASE NOTE: discounted prices are not available on purchases using 0% finance or a Cycle to Work Scheme. Discounted prices are not available in conjunction with any other promotion.


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At its core, the Summit invites all of us to connect with the remarkable possibility and passion of teaching.
On the Saturday before the College Football Playoff National Championship, we will take a time out to focus on the people who helped all the student-athletes out there reach their dreams of attending college: the teachers. Imagine a fast-paced, half-day gathering of teachers coming together to hear inspiring speakers and incredible performances who are all there for one reason: to recognize the hard work of those on the front lines of educating our kids. Think of this as the half-time celebration for teachers coming back from winter break into the second half of their year; the day will be engaging and energetic, and all about putting teachers center stage.
In 2018, we're headed to Atlanta! We'll be at the Georgia World Congress on Saturday, January 6th.


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Sew-Rite 8101; ca. late 1940s or early 1950s; Half Size Apron Dress and Cobbler Apron with Flower Petal Pocket. 6 pieces
Links to reviews/blog postsEdit
Add a photo to the gallery by clicking the "modify" button below.
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Joe Mauer's grandfather: 'He's getting a big salary, he should produce'
Add Joe Mauer's grandfather to those piling on the Twins' franchise player for his lack of production.
More MLB: Power Rankings | Expert Picks | Latest news & notes | Fantasy news
Things have not gone well for Twins franchise player Joe Mauer this season, his first as a full-time first baseman. He is currently on the disabled list with an oblique problem and is hitting an un-Mauer-like .271/.342/.353 (95 OPS+).
The Twins are pretty bad (41-49, last place in the AL Central) for the fourth year in a row and Mauer has heard more than his fair share of boos from the Target Field faithful. That's usually how it goes, the team stinks and the star player hears it the most.
Among those who understand the fans' frustration and the booing is Jake Mauer, Joe's grandfather. Here's what he told David Shama earlier this week (h/t Star Tribune):
"He's getting a big salary, he should produce," Jake said. "That's what the fans think and that's what the fans want. He's trying but it just don't happen (yet). But I don't blame the people."
"He couldn't understand the (poor hitting) stretch that he went through," Jake said. "He's never had it in his life. He starts coming out of it and then he gets hurt.
"He says, 'What the heavens are going to happen next? Here I suffer for two months and then I start a string of going good and then I get hurt. It's just terrible.' "
Mauer, 31, will earn $23 million this season and is owed another $92 million from 2015-18 as part of his catcher record eight-year, $184 million contract. The team moved him to first base this year in an effort to keep him healthy long-term. Catching is a rather brutal job.
Mauer hit .324/.404/.476 (142 OPS+) just last season and put up an astounding .328/.411/.474 (141 OPS+) batting line from 2008-13. He was -- and still is despite this year's hiccup, in my opinion -- on the Hall of Fame track and is line to go down as the greatest player in Twins franchise history.
But, when you're owed a ton of money and are having a poor statistical season, you're going to hear it from the fans, especially when the team is bad overall. Mauer knows it, his grandfather knows it, everyone knows it. It comes with the territory.


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Identification and analysis of murine pancreatic islet enhancers
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The paucity of information on the epigenetic barriers that are blocking reprogramming protocols, and on what makes a beta cell unique, has hampered efforts to develop novel beta cell sources. Here, we aimed to identify enhancers in pancreatic islets, to understand their developmental ontologies, and to identify enhancers unique to islets to increase our understanding of islet-specific gene expression.
We combined H3K4me1-based nucleosome predictions with pancreatic and duodenal homeobox 1 (PDX1), neurogenic differentiation 1 (NEUROD1), v-Maf musculoaponeurotic fibrosarcoma oncogene family, protein A (MAFA) and forkhead box A2 (FOXA2) occupancy data to identify enhancers in mouse islets.
We identified 22,223 putative enhancer loci in in vivo mouse islets. Our validation experiments suggest that nearly half of these loci are active in regulating islet gene expression, with the remaining regions probably poised for activity. We showed that these loci have at least nine developmental ontologies, and that islet enhancers predominately acquire H3K4me1 during differentiation. We next discriminated 1,799 enhancers unique to islets and showed that these islet-specific enhancers have reduced association with annotated genes, and identified a subset that are instead associated with novel islet-specific long non-coding RNAs (lncRNAs).
Our results indicate that genes with islet-specific expression and function tend to have enhancers devoid of histone methylation marks or, less often, that are bivalent or repressed, in embryonic stem cells and liver. Further, we identify a subset of enhancers unique to islets that are associated with novel islet-specific genes and lncRNAs. We anticipate that these data will facilitate the development of novel sources of functional beta cell mass.
KeywordsChIP-seq Enhancer H3K4me1 lncRNA Pancreas Transcription factor
- ChIP-qPCR
Chromatin immunoprecipitation with quantitative PCR
- ChIP-seq
Chromatin immunoprecipitation sequencing
Embryonic stem cell
Forkhead box A2
Gene ontology
- H3K4me1
Histone H3-lysine 4 monomethylation
- H3K4me3
Histone H3-lysine 4 trimethylation
- H3K9me3
Histone H3-lysine 9 trimethylation
- H3K27me3
Histone H3-lysine 27 trimethylation
- H3K27ac
Histone H3-lysine 27 acetylation
Islet-specific enhancer
Kyoto Encyclopedia of Genes and Genomes
- lncRNA
Long non-coding RNA
v-Maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
Neurogenic differentiation 1
Non-islet-specific enhancer
Pancreatic and duodenal homeobox 1
- PhyloCSF
Phylogenetic codon substitution frequency
- RNA-seq
RNA sequencing
Serial analysis of gene expression
Transcriptional start site
University of California, Santa Cruz
Prevention of diabetes depends on maintaining beta cell mass and insulin-secretory capacity. For this reason, recent efforts have focused on finding ways of enhancing beta cell survival, preventing beta cell death, and stimulating replacement of beta cell mass. One strategy involves the reprogramming of embryonic stem cells (ESCs) or more abundant cell types, such as hepatocytes from the liver, which, like the pancreas, is derived from the foregut endoderm [1], into glucose-responsive insulin-secreting cells. However, such protocols often generate multihormonal cells, predominately produce alpha cells, or generate 'beta cells' that cannot match the insulin-secretory capacity of a native beta cell. In part, progress in developing better protocols is hampered by our lack of understanding of the regulatory networks that drive beta cell genesis and function, by our inability to assess how closely protocols recapitulate normal beta cell development, and by our limited awareness of the epigenetic barriers that are being encountered during reprogramming.
As enhancers largely govern tissue-specific gene expression [2], we anticipated that identifying enhancers in islets and analysing their chromatin states in ESCs, hepatocytes and islets would be of particular value for improving protocols for generating beta cells, and would provide novel insights into beta cell development and function. To date, enhancer loci have been detected genome-wide using transcription factor binding [3, 4] or histone modification enrichment data [2, 5], or by identifying regions of open chromatin [6, 7]. Although each of these approaches has specific benefits, they either suffer from a high rate of false positives or are unable to detect the majority of enhancers. We therefore sought to develop a novel combined approach to identifying enhancers that would take advantage of the benefits of each method, while mitigating their limitations.
For this, we combined nucleosome predictions based on histone H3 lysine-4 monomethylation (H3K4me1) enrichment data, which demarcates active and poised enhancer loci [8, 9, 10, 11], with genome-wide occupancy data for pancreatic and duodenal homeobox 1 (PDX1), v-MAF musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (MAFA), neurogenic differentiation 1 (NEUROD1) and forkhead box A2 (FOXA2), which are critical regulators of beta cell development and function [12, 13, 14, 15], to identify enhancers in in vivo mouse pancreatic islets. Using these data, we identified 22,223 putative enhancer loci. We then compared the chromatin states of these loci in ESCs and hepatocytes in order to assess their developmental ontologies and to begin to understand the types of epigenetic barriers faced by protocols using these cell types to generate beta cells. Finally, we identified enhancers unique to islets in the hope of gaining additional insight into the transcriptional networks that make beta cells different from other cell types.
Chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq)
We performed ChIP-seq using antibodies to MAFA (Abcam, Toronto, ON, Canada) and NEUROD1 (Santa Cruz, Santa Cruz, CA, USA) using freshly isolated 8–10-week-old mouse islets, and to histone H3 lysine-9 trimethylation (H3K9me3) (Millipore, Billerica, MA, USA) and histone H3 lysine-27 trimethylation (H3K27me3) (Millipore) in islets and liver as previously described [8, 16]. RNA-seq was performed as previously described [17] using pooled islets from C57BL/6J mice with two replicates sequenced. For more information on mouse maintenance, islet isolations, ChIP-seq and RNA-seq, see electronic supplementary material (ESM) Methods. Data were deposited under GEO accession GSE30298.
Analysis of ChIP-seq datasets and detection of H3K4me1-marked nucleosome locations
Probabilistic inference for nucleosome positioning (PING) [18] was used to identify H3K4me1-marked nucleosome positions from sonicated H3K4me1 ChIP-seq data in pancreatic islets. Sequence reads from sonication-based ChIP-seq experiments for H3K4me1, histone H3 lysine-4 trimethylation (H3K4me3), H3K9me3 and H3K27me3 in ESCs, for H3K4me1 and H3K4me3 ChIP-seq experiments in islets and liver, and for PDX1 and FOXA2 in islets were obtained from previously generated data (ESM Table 1) [8, 19, 20, 21, 22, 23, 24, 25]. Reads were mapped and peaks identified as described in ESM Methods. Clustering of histone modification data was performed using the total read counts in 2 kb windows around locus midpoints.
Chromatin immunoprecipitation with quantitative PCR (ChIP-qPCR)
For ChIP-qPCR, we performed ChIP on cells using 3 μg of anti-H3K4me1 (Abcam), anti-histone H4 acetylation (H4ac) (Millipore), anti-E1A binding protein p300 (p300) (Santa Cruz), anti-histone H3-lysine 27 acetylation (H3K27ac) (Millipore), or rabbit IgG (Santa Cruz). DNA from triplicate ChIP experiments was obtained and amplified using an ABI Viia7 real-time PCR system (Applied Biosystems, Carlsbad, CA, USA) and SYBR Green Supermix (Applied Biosystems). The fold enrichment of each target site was calculated as 2−∆Ct between rabbit IgG and the immunoprecipitated samples. Primers were designed using Primer3. Primer sequences are available upon request.
Dual luciferase assays
We cloned selected loci from mouse islet DNA into pGL 4.23 (Promega, Madison, WI, USA). Cells were seeded in 96-well plates and co-transfected with 40 fmol pGL4-enhancer firefly and 0.7 fmol Renilla (pRL-TK; Promega) vectors using Lipofectamine 2000 (Life Technologies, Burlington, ON, Canada). Six replicate transfections were performed for each cell line. Cell lysates were prepared and analysed according to the manufacturer's instructions using the Dual-Luciferase Reporter Assay System (Promega). Firefly values were normalised to Renilla. Activity was defined as 2SD (p < 0.01) above the median activity of negative controls.
Identification of novel transcripts in islets
To identify novel transcripts expressed in islets, we performed de novo assembly of islet RNA-seq reads (ESM Methods) using Trans-ABySS [26]. Contigs were aligned to the NCBIM.37 reference genome, and alignments were annotated against Ensembl v64 genes. Contigs with alignments that did not overlap an annotated gene in this database were filtered (ESM Methods), and the coding potential of the remaining transcripts was determined using phylogenetic codon substitution frequency (PhyloCSF) [27] using an eight-way multispecies alignment. Transcripts with a PhyloCSF score below 100 were considered non-coding [28].
The expression (reads per kilobase per million mapped reads) of the contigs in the islet RNA-seq dataset and in 14 other tissues [25] (ESM Table 1) was calculated by determining the number of reads with a minimum quality score of 10 that overlapped each exon using SAMtools [29]. At least three reads had to overlap a contig in a library for it to be considered to be expressed.
qPCR validation of novel transcripts
RNA was isolated from adult tissues using Trizol (Life Technologies) and RNA purification columns. All primers were designed using Primer3Plus and spanned introns where possible; primer sequences are available on request. A Viia7 real-time PCR system (Applied Biosystems) and Fast SYBR Green Master Mix (Applied Biosystems) was used for all reactions. Triplicate cDNAs were obtained by reverse transcription of 1 μg total RNA from newly isolated tissue. A 10 ng amount of generated cDNA was used in each reaction, and all reactions were performed in triplicate. Obtained values were normalised to β-actin Ct values to determine the percentage abundance relative to β-actin in each sample.
Genome-wide identification of putative enhancers in pancreatic islets
As a first step to identifying enhancers in mouse pancreatic islets, we identified in vivo locations of H3K4me1-enriched nucleosomes genome-wide [8, 18]. From this, using a strict definition of an enhancer and a stringent set of selection criteria chosen to minimise false-positive regions (ESM Methods and ESM Fig. 1a), we identified 16,835 putative enhancer loci. We identified additional loci using 13,770 PDX1-occupied and 6,176 FOXA2-occupied loci identified using previously generated ChIP-Seq data [8], as well as 3,638 MAFA-occupied and 6,568 NEUROD1-occupied loci using newly generated ChIP-seq data from mouse islets. Combining these data allowed us to identify 22,223 putative enhancer loci in in vivo islets flanked by H3K4me1-marked nucleosomes (ESM Fig. 1a, b and ESM Table 2).
Enhancer loci have distinct developmental ontologies
We next compared the frequency with which PDX1, MAFA, NEUROD1 and FOXA2 occupied the enhancers in the different clusters. In general, the enhancer clusters had similar occupancy frequencies (Fig. 2c, d), except for clusters e5 and e8, which had reduced occupancy levels compared with the other clusters (1.6-fold decrease, p < 0.0001, Fisher's exact test). On the other hand, when we compared the motifs enriched in the clusters (ESM Methods), we found that cluster e1, which was in an active state in ESCs, liver and islets, was highly enriched for motifs for widely produced transcription factors (Fig. 2e), while cluster e3, which was in an active state in liver and islets, was highly enriched for motifs for transcription factors produced in both islets and liver, and clusters e4, e5 and e6, which are predominately in an active state in islets only, were enriched for motifs for transcription factors produced in the islet lineage.
Enhancers with different developmental ontologies regulate genes with distinct attributes
Identification of islet-specific enhancer (ISE) regions
To begin to determine why the identified ISEs obtained an active chromatin state uniquely in islets, we compared their underlying DNA sequence with NSEs. ISEs had a significantly lower (p < 0.0001) average GC content than NSEs (Fig. 4e). In agreement, ISEs were enriched for HNF3-, PAX-, NKX-, RFX- and PDX1-like motifs (Fig. 4f and ESM Fig. 6, 7), which are A/T rich, and were specifically deprived in ZFX-, SP-, AP- and CTCF-like motifs, which are more G/C rich (ESM Methods). Comparing the spatial distributions of ISEs and NSEs, we observed that ISEs had a broader distribution around transcriptional start sites (TSSs), while NSEs were more often proximal to TSSs (Fig. 4g); in fact, the mean enhancer to TSS distance was over three times higher for ISEs than NSEs (p < 0.0001). This resulted in significantly fewer ISEs than NSEs being mapped to known genes (p < 0.0001) (Fig. 4h).
ISEs are associated with novel islet-specific transcripts
To determine whether the ISEs that we were unable to map to any known genes (Fig. 4h) might regulate novel transcripts, such as long non-coding RNAs (lncRNAs), we used Trans-ABySS [26] to perform de novo assembly of the islet RNA-seq reads. Trans-ABySS identified 2,498 transcripts that met our minimum read count and exon count thresholds (ESM Methods) and that had no annotation in the Ensembl, Refseq or University of California, Santa Cruz (UCSC) databases (ESM Table 3). The expression of these transcripts was significantly lower than for known protein-coding genes (p < 0.0001), as previously reported for non-coding transcripts in other tissues [28]. These 2,498 transcripts represented 1,473 distinct loci, suggesting that some of the loci may generate multiple transcriptional variants, or that complete transcripts were not constructed by Trans-ABySS because of the low read counts associated with these transcripts [28, 36]. Some 92% of these transcripts were associated with H3K4me1/H3K4me3-enriched regions, and 78% could be associated with an identified islet enhancer, further suggesting their validity.
As enhancers are thought to be the primary regulators of tissue-specific gene expression [2, 9], we attempted to develop a compendium of in vivo enhancer elements in pancreatic islets. We subsequently sought to determine how the developmental ontologies of the enhancer loci might affect protocols for generating beta-like cells from ESCs and hepatocytes, and to identify enhancers associated with islet-specific transcriptional networks.
In an effort to mitigate the limitations of previous approaches to identifying enhancers genome-wide [2, 3, 4, 5, 6, 7], we combined predictions of H3K4me1-marked nucleosomes with locations of PDX1, NEUROD1, MAFA and FOXA2 binding in vivo to identify enhancer regions in islets. This approach ensured that the loci identified were flanked by H3K4me1-marked nucleosomes, and were therefore both within open chromatin and in an appropriate chromatin state [9, 10, 11]. Despite this, it is possible that the identified loci could contain central unmarked nucleosomes [37], although such central nucleosomes would probably contain the histone variants, H3.3 and/or H2A.Z, that are common within regulatory regions [38]. Further, by using H3K4me1, rather than H3K27ac, which only marks active loci [21, 39], we were able to identify loci both in an active state and in a poised state that may become active under different physiological conditions. Meanwhile, including PDX1, NEUROD1, MAFA and FOXA2 occupancy data allowed us to identify additional loci lost because of the stringency of our selection criteria using H3K4me1-marked nucleosome-based predictions.
Despite the benefits of our approach, we note that the 22,223 loci identified here do not represent a fully comprehensive list of enhancers in islets, in part because the stringent criteria used probably eliminated many true enhancers. In addition, mouse islets are predominately composed of beta cells (∼80%), and PDX1, MAFA and NEUROD1 are found only in beta cells in the adult, suggesting that the majority of loci identified here are probably beta cell enhancers, and enhancers from other islet cell types (alpha cells, delta cells, PP cells and epsilon cells) are probably largely not detected. Regardless, our data suggest that roughly 50% of the regions identified are active, a higher fraction than reported in other efforts to identify functional enhancers in HeLa cells using histone modification data (∼36%) [2], or in islets using open chromatin (∼33%) [7], validating the relative success of our approach. Thus, we think it likely that our list of putative enhancers in islets, while not exhaustive, is highly enriched in loci capable of acting as functional enhancer elements in beta cells, and should be of significant utility in generating novel biological insights.
Although previous efforts to identify cis-regulatory loci genome-wide in human islets have proven valuable [6, 7], these studies were unable to provide insight into the developmental ontologies of the loci. To begin to address this, we compared the chromatin states of the identified enhancers in islets with their chromatin states in ESCs, which can be used to infer the chromatin state of the loci before differentiation, and in liver, which, like the pancreas, develops from the foregut endoderm and thus can be used to infer whether a specific chromatin-state transition occurred before pancreas/liver specification. We found that enhancers in islets are generated through at least nine distinct developmental ontologies. Of these, we identified three ontologies associated with genes that have islet-specific expression and function. This included enhancers that uniquely obtained H3K4me1/H3K4me3 marks in islets, as well as those that were in a repressed state in ESCS, and those in a bivalent state in ESCs, but in an active state in islets. Our results agree with previous work indicating H3K27me3 prepatterns Pdx1 regulatory regions [40], and further show that genes for many of the transcription factors important in pancreas development, including Nkx2-2, Nkx6-1, Mafa and Mnx1, also have enhancers that were either bivalent or repressed in ESCs but in an active state in islets. Together, these results suggest that the primary epigenetic barriers faced in the conversion of ESCs and hepatocytes into beta-like cells are the appropriate recruitment of trithorax complexes, which can induce H3K4 methylation [41], and H3K27me3 demethylases, such as KDM1 lysine (K)-specific demethylase 6B (KDM6B, also known as JMJD3) [42], to islet-critical cis-regulatory regions.
To further define islet-specific transcriptional networks, we next discriminated 1,799 enhancers unique to islets using H3K4me1 data from 19 other cell or tissue types. We found that these enhancers have a reduced average GC content compared with NSEs, and were enriched for A/T-rich motifs for pancreas-specific transcription factors. Although we found ISEs associated with several known islet critical factors, we also found ISEs associated with many genes with as yet uncharacterised roles in beta cell function but which have islet-enriched expression, including genes involved in RNA splicing, cell adhesion and cytokine-mediated signalling. We further identified several ISEs associated with previously unknown islet-expressed lncRNAs. At least 75% of these lncRNAs were more abundantly expressed in islets than in any other tissue type, suggesting that they are also islet specific. Consistent with this, many lncRNAs are tissue specific [28], and are thought to play roles in regulating tissue-specific transcriptional networks or in establishing the chromatin state of tissue-specific regulatory regions [43]. The roles of these lncRNAs, and of the other genes identified with an associated ISE, in beta cell development and function will be of considerable future interest.
In summary, we identified 22,223 putative enhancer loci in pancreatic islets. We show that these loci have at least nine distinct developmental ontologies, and find that, in contrast with promoters, the majority of enhancers acquire H3K4me1 either specifically in islets or in a shared islet/liver multipotent progenitor. Our analysis of these regions clearly points to the importance of the coordinated, stage-specific action of histone methyltransferases and histone demethylases in establishing appropriate chromatin states at enhancers and promoters that regulate genes critical to pancreas specification and beta cell function. Further, we identify 1,799 of these loci as unique to islets, and show the utility of these data by using them to help identify novel islet-specific lncRNAs. We anticipate that our data will contribute towards ongoing efforts to understand beta cell development and function, and will facilitate the development of novel strategies for generating glucose-responsive, insulin-secreting cells.
The authors would like to acknowledge Canada's Michael Smith Genome Sciences Centre's (BCGSC) sequencing, bioinformatics and SAGE library construction teams, as well as P. Plettner (BCGSC) and A. He (BCGSC) for technical assistance, and J. Johnson (BCGSC) and A. Kotzer (Terry Fox Research Institute) for project management.
M.A. Marra, P.A. Hoodless and S.J.M. Jones are Senior Scholars of the Michael Smith Foundation for Health Research. L. Li was supported by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences (Z01-ES-101765). R. Gottardo was supported by NIH grant HG005692. X. Zhang was supported by an NSERC-CGS fellowship. F.C. Lynn was supported by the Canadian Institutes for Health Research (MOP-102628 and RMF-111626) and the Juvenile Diabetes Research Foundation (2-2011-91). Funding was provided by Genome Canada, Genome British Columbia, the Child and Family Research Institute, the Juvenile Diabetes Research Foundation (5-2011-85), Common Wealth Insurance, the Canadian Institutes for Health Research (MOP-111010), and the Canucks for Kids Foundation, with infrastructure support provided by the British Columbia Cancer Foundation.
Duality of interest
The authors declare that there is no duality of interest associated with this manuscript.
Contribution statement
BRT, MK, CJW, AK and BGH were involved in the acquisition and analysis of the ChIP-qPCR, qPCR and luciferase data. MB, PVS, FCL, MAM, SJMJ and BGH participated in acquisition of the ChIP-seq and/or RNA-seq data, while AGR, LL, XZ, NT, RC, KM and BGH performed the analysis and interpretation of the data. RG, MAM, SJMJ, PAH and BGH conceived and designed the experiments. BRT, AGR and BGH wrote the manuscript. MK, MB, CJW, PVS, AK, LL, XZ, NT, RC, KM, RG, MAM, FCL, SJMJ, and PAH provided critical revisions for important intellectual content. All authors approved the final version.


text:
Bollinger, 25, is currently enjoying an excellent season with New South Wales having claimed 22 wickets in five Pura Cup matches at an average of 24.68.
He joins fellow NSW star Phil Jaques at Worcester as the county's second overseas player.
Steve Rhodes, the county's director of cricket, said: "We have been looking for a left arm fast bowler to give balance to our attack who is available for the whole season. Doug fulfils all those criteria, has a strong work ethic and is currently enjoying an excellent season with New South Wales.
"I have personally spoken to both Phil Jaques and Glenn McGrath and they speak very highly of him. Our success last season was a result of our strong team ethic and everything I have heard about Doug leads me to believe he will fit in superbly."
"I have heard a lot of good things about Worcestershire and am really looking forward to joining Phil Jaques and the rest of the team at New Road in April. This is a great opportunity for me to progress my career and I can assure all the Worcestershire supporters that they will get 100 percent effort from me," said elated Bollinger.


text:
Get an overview of this hotelJinhua hotel in Jinhua City Centre with restaurant and business center
Popular property highlights
Located in Jinhua City Centre, this hotel is within 3 mi (5 km) of Wuzhou Park, Jinhua Grand Theatre, and Jinhua Municipal People's Government. Lan River is 19.1 mi (30.7 km) away.
Hotel Features
A restaurant, a business center, and dry cleaning are available at this hotel. WiFi in public areas is free. Additionally, a 24-hour front desk, express check-in, and express check-out are onsite.
Room Amenities
All 96 rooms offer free wired Internet, free bottled water, and phones. Desks and free toiletries are also available to guests.Information missing or incorrect? Tell us!Opens in a new window
GreenTree Inn Jinhua Railway Station Hotel
Hotel Amenities
Hotel Amenities
The hotel offers a restaurant. Public areas are equipped with complimentary wireless Internet access. A business center is on site. This Jinhua hotel also offers tour/ticket assistance, complimentary newspapers in the lobby, and dry cleaning/laundry services.
GreenTree Inn Jinhua Railway Station Hotel has designated areas for smoking.
- Total number of rooms - 96
- Number of floors - 7
- Elevator/lift
- Restaurant
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- Express check-out
- Dry cleaning/laundry service
- Breakfast available (surcharge)
- Television in common areas
- Safe-deposit box at front desk
- Tours/ticket assistance
- Free WiFi
- Year Built 2008
- Number of buildings/towers - 1
Available in all rooms: Free wired Internet
Available in some public areas: Free WiFi
Room Amenities
- Air conditioning
- Phone
- Free toiletries
- Free bottled water
- Blackout drapes/curtains
- Slippers
- Hair dryer (on request)
- Shower/tub combination
- Free wired Internet
- Desk
Where to Eat
Buffet breakfasts are available for a surcharge and are served each morning between 7 AM and 9 AM.
GreenTree Inn Jinhua Railway Station Hotel has a restaurant on site.
If you have requests for specific accessibility needs, please note them at check-out when you book your room.
- In-room accessibility
Hotel Policies
Check-in time starts at 2 PM
Check-out time is noon
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Children and extra beds
- Children are welcome.
- Rollaway/extra beds are not available.
- Cribs (infant beds) are not available.
- No pets or service animals allowed
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
No rollaway/extra beds available
No cribs (infant beds) available
No pets or service animals allowed
Optional extras
- Fee for buffet breakfast: CNY 10 per person (approximately)
Hotel Name
- GreenTree Inn Jinhua Railway Station
- GreenTree Inn Jinhua Railway Station Hotel
We should mention
Only registered guests are allowed in the guestrooms. No pets and no service animals are allowed at this property.
National Rating
For the benefit of our customers, we have provided a rating based on our rating system.


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IFC, a member of the World Bank Group, and the IFC Middle East and North Africa Fund, managed by IFC Asset Management Company, today announced a combined indirect equity investment of $30 million via the WP/GA consortium fund in Network International, a leading provider of payment solutions, to expand the payment infrastructure in the Middle East and Africa.
IFC and the MENA Fund have each invested $15 million to acquire indirect stakes in Network International, which is jointly controlled by the Emirates NBD Bank and Warburg Pincus/General Atlantic consortium. The investment will help the company expand and modernize its banking client network in the Middle East and Africa, by helping them develop electronic channels, improve their level of service, and expand their reach into underserved segments, boosting financial inclusion.
Simon Haslam, Group CEO of Network International, said: "This investment is testimony to Network International's position as the leading payment solutions provider in the Middle East and Africa, operating across 71 countries, servicing over 200 banks and more than 70,000 merchants. It will help Network International as it continues on its path to growth with new and exciting products and services harnessing the latest technology."
According to McKinsey, credit card, debit card and ATM penetration in the Middle East and Africa is the lowest in the world, with only 0.25 cards per adult and 97 percent of all transactions still cash based. Only 31 percent of the population is banked, while more than 30 million micro, small and medium enterprises remain unserved or underserved.
"Network International is well positioned to offer affordable and innovative solutions to merchants, banks and consumers, strengthening the payment infrastructure," said Bassel Hamwi, Head of the IFC Middle East and North Africa Fund. "A shared infrastructure brings down costs and boosts financial inclusion, while reducing the risk of fraud."
The investment will allow MSMEs to access card-based payments and develop digital data records, to help them grow their customer base. Network International's expansion will also promote cross-regional integration of the financial and payment infrastructure.
"Network International is our strategic partner in establishing and expanding shared financial infrastructure across the Middle East and Africa," said Andi Dervishi, Global Head of IFC's Fintech Investment Group. "The company will help modernize banks by developing shared electronic channels with an improved level of service and deeper reach into underserved populations, while improving transparency and reducing money laundering, tax evasion and terrorist financing."
IFC's support of Network International is part of a broader strategy in the Middle East and North Africa to address long-term development challenges and increase employment opportunities through the private sector. During fiscal year 2016, IFC committed over $1.3 billion in MENA, including $330 million in mobilization. IFC's advisory services approved 20 new projects with a total value of over $21 million.
Communications 2020
July 18 - 20, 2017
Las Vegas, NV
M2M Zone Pavilion @ MWC Americas 2017
September 12-14, 2017
San Francisco, Calif.


text:
Monster, 1
Print advertisment created by Ogilvy, Costa Rica for Kimberly-Clark, within the category: Health.
Do you know what you wash your hands with? One in every four soap refills is contaminated with potentially harmful bacteria.
If it's Kimberly-Clark Professional, hygiene is guaranteed.
General Creative Director: Nicolás Schumacher
Creative Director: Ronny Villalobos
Copywriter: Alonso Cortés
Illustrator: Rodrigo Piedra
Photographer: Ricardo Quirós
Art Directors: Roberto Leitón, Daniel Lopez
I agree with them. Maybe the other agency (Matriz?) did crib the idea.. I believe their ads were published a month later, correct? This is not nice..
I like Ogilvy's campaign more.
On a different note, I don't think that you should stop posting "hand painted" ads! Just because this concept has been around for years doesn't mean we should shelve it.
Another 'Monster Hand' print launched in Singapore Jun 2009
Its a fight between Rodrigo Piedra and Fescher Neoilustração!
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I'm nationally registered and have a job in Utah for a Derm. office. I am trying to finish up my pre-requisites for Nursing also, hoping and and praying to get in next Spring.Jun 24, '12
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TALLAHASSEE — A Senate committee Wednesday unanimously approved a bill inspired by Corey Jones that would require law-enforcement agencies to set policies if they use body cameras.
Jones, a 31-year-old musician, was shot six times by then-Palm Beach Gardens police officer Nouman Raja, who arrived at the scene in plain clothes driving an unmarked white van.
The bill (SB 418), sponsored by Sen. Chris Smith, D-Fort Lauderdale, was approved by the Fiscal Policy Committee and now is ready to go to the full Senate.
The bill would not require law-enforcement agencies to use body cameras but would require standards for those that decide to do so. The legislation has the support of the Florida Sheriffs Association, the Florida Police Chiefs Association and the Florida Police Benevolent Association.
A House version (HB 93), sponsored by Rep. Shevrin Jones, D-West Park, and Rep. Alan Williams, D-Tallahassee, also is ready to go to the full House.
Smith said the use of body cameras helps prevent finger-pointing in police incidents.
"It gives us an opportunity to see what cops encounter a lot of times," Smith said. "When cops come up on people and they are belligerent and they are escalating, it can show that 'Hey, this officer was just reacting to something that they came upon.' But, it shows sometimes when officers are having a bad day and do bad things."


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|Lawrence Page
Mr. Page
|Computer scientist, Internet entrepreneur
| University of Michigan (B.S.)
Stanford University (M.S.) Stanford University (PhD) (dropped out)
|Known for
|Co-founder of Google Inc., CEO of Alphabet Inc, PageRank
|March 26, 1973
Lawrence "Larry" Page (born March 26, 1973) is an American computer scientist and internet entrepreneur who co-founded Google Inc. with Sergey Brin, and is the CEO of Google's parent company,Alphabet Inc. After stepping aside as CEO in August 2001 in favour of Eric Schmidt, Page re-assumed the role in April 2011. He announced his intention to step aside a second time in July 2015 to become CEO of Alphabet, under which Google's assets would be re-organised. Under Page, Alphabet is seeking to deliver major advancements in a variety of industries. Page is the inventor of PageRank, Google's best-known search ranking algorithm.
Page is a board member of the X Prize Foundation (XPRIZE) and was elected to the National Academy of Engineering in 2004. Page received the Marconi Prize in 2004.
Incorporates material from Wikipedia


text:
Dirk Richter
From Wikipedia, the free encyclopedia
|Personal information
|12 September 1964 (age 52)
Cottbus, Brandenburg, East Germany
Medal record
Dirk Richter (born 12 September 1964 in Cottbus, Brandenburg) is a former swimmer from East Germany, who won the bronze medal in the 4×100 freestyle medley twice during the Summer Olympics. He did so in 1988 with the East German team, alongside Thomas Flemming, Lars Hinneburg, and Steffen Zesner.
Four years later, when Barcelona, Spain hosted the Games, Richter represented Germany and ended up third alongside Mark Pinger, Christian Tröger, and Steffen Zesner.
- Dirk Richter at databaseOlympics.com at the Wayback Machine (archived 2007-02-19)
- "Dirk Richter". Olympics at Sports-Reference.com. Sports Reference LLC.
|This article about a swimming Olympic medalist for Germany is a stub. You can help Wikipedia by expanding it.
- 1964 births
- Living people
- German male swimmers
- Male backstroke swimmers
- Olympic swimmers of East Germany
- Olympic swimmers of Germany
- Swimmers at the 1988 Summer Olympics
- Swimmers at the 1992 Summer Olympics
- Olympic bronze medalists for East Germany
- Olympic bronze medalists for Germany
- Sportspeople from Cottbus
- Olympic bronze medalists in swimming
- Male freestyle swimmers
- World Aquatics Championships medalists in swimming
- European Aquatics Championships medalists in swimming
- Medalists at the 1992 Summer Olympics
- Medalists at the 1988 Summer Olympics
- German swimming Olympic medalist stubs


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Owner and Director
Providing ICT consultation to schools, university and private/public organisations. Providing a Moodle site which provides a complete service: maintenance, training and support. www.nordicinteractivesolutions.com
Providing ICT consultation to schools, university and private/public organisations. Providing a Moodle site which provides a complete service: maintenance, training and support. www.nordicinteractivesolutions.com
Providing ICT support for 200 staff in all subjects within Arts Faculty. eLearning producer & green screen recordings. Consultation, seminars, presentations and needs analysis for ICT usage. Identification, utilisation and keyboard support as well as production of video support material for identified software and Web 2.0 sites.
Umeå University website: Satish Patel
ICT Tutor Support, Moodle 2 Support, ICT Student Support
Foundation courses at A, B, C level in linguistics based subjects. Courses in linguistics, grammar, academic writing, sociolinguistics, C-Papers, vocabulary development, language history, applied linguistics, young learners, EU studies.
Additional tasks
Linguistics orientation, focusing on text analysis, genre and corpus studies of corporate annual reports. PhD thesis work, and research courses (equivalent to 80p) in language history, syntax, semantics, linguistics theory, language history, bilingualism, translation, pragmatics, discourse analysis
Additional Tasks (teaching)
The main focus during these 6 years was my family. Parental leave was interlaced with university studies, occasional short-term teaching contracts, and travel, summarised as follows:
Development of teacher training programs via the State Institute for Pedagogical Development and Ostrava University. Distance (using traditional methods) and on-site workshops and seminars for trainee teachers, in-service training for active teachers and unemployed teachers throughout Slovakia. Long term strategic planning for courses and workshops with school adminstrators.
Marketing, management and teaching of language courses to general public and to companies in the local region. Development of teacher training courses both in-house and in the local region.
Teaching English for Specific Purposes in local companies and public organisations.
Program for trainee teachers. Formal qualification requirement for teaching in the UK. Qualification has been recognised by Swedish authorities (equivalent to 40p) and provides 'behörighet' for teaching in Sweden (14-19 yr age group)
Adult education, school and company teaching
Equivalent to 120p Technical-Economics Program, according to Swedish authorities.
Since 2002, my educational interest in the usage ICT tools has developed exponentially. Feeling restricted by tools and software available to me for my teaching, I have searched, found and used IT tools (web 2.0) to enhance and to vary my teaching & learning environment. In recent years, blended learning (link to recent presentation) has become the norm for my distance and campus based teaching (link to article on LearningNet). More recently, my focus has shifted to Student Generated Content. To aid & support teaching, research and administration colleagues, I have various websites, such as: Moodle 2 Support, ICT Tutor Support, ICT Student Support. Please feel free to use them and share them with others.
Some more links for presentations
View samples of my presentations
View samples of ICT materials made for my students:
A few weeks each year, I lead an adventure tourism business catered for exchange students & visitors in Sweden. Participants experience a close contact to the remarkable natural environment of Sweden on water, on snow, on ice, and on mountains. These safaris are for the young and the old of a basic level of fitness.


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Saturday, May 10, 2014 7 a.m. to 1 p.m.
Location: Fields Pond Audubon Center, 216 Fields Pond Road, Holden, Maine
For more information: 2079892591; maineaudubon.org/events/spring-fest-3/
Join our celebration of International Migratory Bird Day and the arrival of spring. This year's activities include bird walks, a children's activity area, music, and more. All ages, get out in nature and spend time with us!
Please call 989-2591 with any questions.
Cost: Free.
7 – 8:30 a.m. Bird Walk with Bruce Barker and Jerry Smith
8-9:00 a.m. Photo Exploration w/ Bruce Gray (bring your camera)
9 – 11:00 a.m. Birding by Ear Walk w/ Steve Coleman
10 a.m. – noon Children's Activity Area w/ Stillwater Montessori
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The South Pole: An Account of the Norwegian Antarctic Expedition in the Fram, 1910-1912
Roald Amundsen records his race to be the first man to reach the South Pole. Amundsen's expertise enabled him to succeed where his predecessors, and competitors, did not. His rival Captain Robert F. Scott not only failed to reach the Pole first, but due to poor preparation and miscalculation died with the rest of his party on their return trip. The South Pole remains one of the greatest and most important books on polar exploration.
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Dariusz Inigratte (born January 21, 2016) is one of the adopted sons of Orla and John Inigratte and the quadecaplet brother of Alenka, Marta, Bartosz, Thomas, Katarzyna, Patricia, Piotr, Monika, Wojciech, Klaudia, Magda, Kasia and Kacper. He and his quadecaplet siblings were born in Warsaw, Poland.
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- Father: John Inigratte
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ARLINGTON, Texas -- Warren Sapp didn't pull any punches Tuesday, when asked about the winter storm that hit North Texas on Super Bowl XLV Media Day.
Someone asked Sapp, the former all-pro defensive tackle who now works as an analyst for the NFL Network, if he thought the Dallas area had put its best foot forward in dealing with ice and light snow that paralyzed the region.
"Dallas has got something to do with the snow?" Sapp said. "I thought it was an act of God."
Following up, a local TV reporter asked Sapp if he thought the storm -- nearly a year after a snow storm struck as the region hosted NBA All-Star Weekend -- would jeopardize the Dallas area's chances to land another Super Bowl.
"You from Dallas?" Sapp said. "What do you think about y'all putting your best foot forward?
"I think y'all need to get a couple more plows. It snowed last year for the all-star game and there were 100,000 people here. Y'all might want to get a couple more snow plows. Y'all don't have a five-day forecast around here? I'm just wondering."
Cowboys owner Jerry Jones later said he was confident that Cowboys Stadium could compete for more Super Bowls.
"This is an inordinate weather event," he said.
Nate Davis is a reporter, blogger and editor who's been at USA TODAY since 2000. He has covered the NFL since 2005. No, he did not play quarterback for Ball State. Davis' succession of our esteemed colleague Sean Leahy at The Huddle is considered a Brady-for-Bledsoe swap by most "insiders."More about Nate


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The Snohomish Municipal Code is current through Ordinance 2326, passed April 4, 2017.
Disclaimer: The city clerk's office has the official version of the Snohomish Municipal Code. Users should contact the city clerk's office for ordinances passed subsequent to the ordinance cited here.
City Website: http://www.snohomishwa.gov/
City Telephone: (360) 568-3115


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Unlocking Africa's Fruitfulness
Monday, 20 August 2012
Water tanks
Store room going up
Store room again
Water tanks ready for collection
Water tanks were purchased for the chicken farmers in the community and a Beyond Belief storeroom is being built at Crossroads Community Church to help store chicken feed and other items that are bought in bulk.
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Wednesday, 18 April 2012
Beyond Belief Tomatoes
Mcebisi Ncube
Mcebisi works with the Beyond Belief farmers who are involved in crop farming. This is what he had to say about the farmers.....
There are 108 Beyond Belief tomato farmers in the communities of Mablauwuni, Natisa, Lushumbe, Gqalaza, Mapane and Silozwe. They have all grown tomatoes which are being collected at the moment and will be going to a market in Bulawayo. The main challenge faced by the farmers was red spider mite and boll worm.
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Monday, 16 April 2012
Update on chickens from Mxwayisi Dube
Mxwayisi Dube
The first group of 7 layer chicken farmers are in their last month with the layer chickens. In June 2012, each farmer will receive 1000 broiler chickens.
The second group of 6 chicken farmers are doing okay. They are up to 93% with the outlay of eggs.
Beyond Belief is blessing 10 farmers a month with 1000 broiler chickens.
The number of farmers has increased. The aim is to work with 100 farmers. So far, Beyond Belief has 35 in Mablauwuni Village and the rest will come from the surrounding community of Silozwe.
Generally, the chickens are doing real well and theft in the community has stopped.
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Data from: Senescence in immune priming and attractiveness in a beetle
Date Published: March 30, 2012
DOI: http://dx.doi.org/10.5061/dryad.434pp11q
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|Senescence of immunity in Tenebrio molitor - J Evol Biol
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|This excel file contains the data on encapsulation response of young and old males and their attractiveness. The attractiveness was measured as the total time females spent (s) and number of visits of females on pheromone discs of the males.
|Senescence of immunity in Tenebrio molitor....xlsx (11.69 Kb)
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