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Downloads \ Street Legal Racing: Redline \ Cars \ Toyota Corolla Sprinter Trueno AE86Author / Submitted by: JDW, GOM
Toyota Corolla Sprinter Trueno AE86
Beta 0.3c
Original mod JDW team
Fixes, modifications, parts added Davidov
Engine TimEK9, modified by JDW
Special thanks JAG, theREALionKING
Levin parts by JDW team and BiggBoss93
Okay, long story short: JDW was working on this for a long time. They released betas 0.1 and 0.2. The latter was "fixed" and re-released by JAG who also added a few extra parts. Beta 0.3 was never released officially. I took the liberty of fixing some things with the car and engine, adding stuff, etc. Nobody knows what happened to the original JDW project. I don't remember where I had the 0.3 beta from, but I have it.
Now I'm re-releasing this as Beta 0.3a.
Everything is in the proper catalog section.
Suspension scripted
Stock parts scripted (now it comes fully built from the catalog)
Added paintable Kouki and Zenki front bumpers from JAG's o.2
Added paintable rear bumper from JAG's 0.2
Added ITB and filters from the original 4A-GE by TimEK9
Fixed some scripts for the engine
Fixed some scripts for the car
Got rid of "supercharger" bug.
Added exhaust for those who don't wish to use the 4A-GE and/or want custom mufflers.
Fixed ITBs.
Added Levin parts and chassis.
Fixed bug where only the Zenki model would be updated. Note: now the car model's .cfgs are in AE86/cfg, not AE86/Trueno/Zenki/cfg; AE86/Trueno/Kouki/cfg and AE86/Levin/cfg!!!
Added custom adjustable transmission.
Added TRD engine block for higher RPM. Note: you must use appropriate parts in order to go up to 11000 RPM. Also note the engine durability is fairly low, so use it wisely)!!!
Added silver-top valve and timing covers.
Added supercharger.
Known bugs:
ChargeSpeed bumper has mapping errors.
Levin chassis has some mesh bugs.
Levin front bumper plastic is lighter.
Not all light glows done.
Supercharger can work without the appropriate belt.
Tested on 2.2.1. MWM with LE2MWM mod.
Note: Trueno bumpers can be mounted on the Levin, but they will require scripting.
Tags: No tags
Download: Toyota Corolla Sprinter Trueno AE86
Filesize, kB: 33KB
Downloaded: 20541
2017-07-07 10:38
Beatiful, thanks!
2017-05-22 09:37
where is the trd engine block? im trying to replicate takumi ae86 but i cant fine the trd engine block
2017-05-18 06:12
slrr editor errors says no cfg (levin_coupe_zenki.cfg). slight oversight?
2016-05-28 16:32
i cant use SLRR editor,it has somekind of .cfg postition problem.
if i put the files where the slrr editor wants then it works
but levin dont take any engine except the trueno
(it does not take the engine that came with the mod too)
The best mod ever,thnks!
could we have a 2.2.1 mwm?
2016-04-03 17:13
This mod is beautiful and very detailed. I love it.
@BYZEUS It means that it´s for 2.3.0 LE and not 2.2.1 MWM.
link is rubbish. download button doesn't work. Mirror plz
can't find engine custom parts
mmm engine has no custom parts
FIX engine and car please Game crashes after install this mods
new link please mediafire if possable
Game crashes after install, any help?
Unregistrable node: parts\engines\Toyota_4A_GE.rpk:0000008F (invalid parent parts.rpk:0000AB5C)
how to fix this?
Total: 111, on page: 15
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in our current inventory
1966 Chevrolet Chevelle 300
- Stock #
- 132296
- Body Style
- Hardtop
- Engine
- Exterior Color
- Hugger Orange
- Interior Color
- Black
- Miles
- 28
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Sometimes, simple is good. This 1966 Chevelle 300 2-door sedan is proof enough of that. Without any flashy graphics, body modifications, and a brilliant Hugger Orange paint job, it stands out not because of what's there, but because of what's not. Of course, to pull off a great car like this one, everything you do has to be flawless, from the bodywork to the powertrain to the interior, because there's nowhere for shoddy workmanship to hide with this one. It's beautiful in photos, but in person, it's flat-out astounding.
First off, kudos to the guy who chose this basic 300 2-door sedan instead of a garden-variety hardtop. There's nothing we like more than a sleeper, although a Hugger Orange cruiser with a 572-inch crate motor is hardly a stealth-mobile. Still, you just can't argue with the clean lines of the 300 series, and this car retains 100% of its original factory sheetmetal, except for the cowl-induction hood, which is a steel reproduction. It was blasted down to bare metal before the reconstruction got started, and the finish was built from there. To get this car as straight as it is must have taken hundreds of man-hours laboriously sanding, filling, fitting, and adjusting to make it look this good. The paint is from PPG, and is so deep and rich that it looks like the body itself was made from some exotic Hugger Orange material and not ordinary steel. The way it seems to glow all by itself simply has to be seen in person. There's no metallic, no pearl, nothing that glitters in the finish, and again, the simple approach simply works—please look at the pictures again and understand that it looks even better in person.
There isn't much trim on the base 300 model, but that totally works for this car, because what remains was restored to concours condition. The bumpers are brilliantly re-chromed, the grille has been straightened and polished, and the stainless window surrounds have been refinished. All the original badges and emblems were retained, from the crossed flags on the front fenders to the block letters on the tail that spell out CHEVELLE, it's exactly as it was 45 years ago—only about a thousand times nicer. New tinted glass was installed all around, but it looks like the old Soft-Ray stuff and not some modern dark tint that would undoubtedly look odd.
So the body is fairly stock, but the powertrain is definitely not. Under that cowl induction hood lives one of the biggest big blocks GM has ever created, a 572 cubic inch monster that cranks out more than 600 horsepower. Breathing through a custom-fabricated air intake, the mammoth big block is a torque factory, and you really shouldn't try to use first or second gear unless the car is aimed where you want to go. Fortunately, the engine bay got all the flash that they left off the body, from the matching Hugger Orange paint on the valve covers, intake, and block, to the polished serpentine drive system with chrome alternator and A/C compressor, to the fabricated and polished aluminum radiator. Wiring has been artfully hidden, and the inner fenders and firewall have been smoothed and finished to the same standards as the rest of the body. Heck, even the bottom of the hood appears to have been wet sanded and buffed, and it looks better than the exterior of most new production cars. And dig how the power brake booster has been integrated into the firewall for a seamless look—too cool! Other trick details include chrome hood hinges, latch hardware, and radiator cradle, polished billet overflow cans, as well as a set of ceramic-coated long-tube headers with positively massive 2.25-inch primaries.
The only transmission man enough to live behind this killer big block is a Tremec 5-speed manual, which is hooked up thanks to a McLeod hydraulic clutch. The transmission case and bellhousing have been fully polished to match the Tremec-prepped driveshaft with heavy-duty U-joints at both ends. Out back, a narrowed Currie 9-inch rear full of 3.50 gears tries its best to put the power down, and has been given a ceramic coating to match the rest of the driveline. Of course, the show-quality detailing continues, with floors that have been finished to match the body and a filled and sanded frame whose seamless curves are covered with satin black chassis paint from Eastwood. Those beautiful headers I mentioned earlier dump into a ceramic coated 3-inch exhaust system featuring Borla stainless steel mufflers. The front suspension uses upper and lower A-arms with QA1 adjustable coil-over shocks, while the rear is suspended on gorgeous billet aluminum Metco control arms. 13-inch Baer brakes are more than up to the task of managing the 572, with slotted, cross-drilled and zinc-coated rotors at all four corners. It rolls on a set of nicely proportioned 17×8 front and 17×10 Budnick billet aluminum wheels wearing Goodyear performance rubber.
Inside, the string of hits just keeps coming with this 300, including low-back buckets from Corbeau, covered in vintage black and orange houndstooth fabric and black vinyl. Custom door panels were fabricated and upholstered to match, and overhead there's a beautifully fitted black headliner. The carpets are black Daytona weave which looks awfully authentic, and the back seat has been custom upholstered to match the front buckets. The entire dash has been reworked, and now features an array of Auto Meter analog gauges ahead of the driver, and a simple control panel for the A/C controls, but little else. A Hurst shifter pokes out of the floor, and in the spirit of the car's "less is more" theme, there's no center console. The steering wheel, pedals, A/C vents, and door handles are all billet aluminum, and there's a cleverly hidden entertainment system with a 10-disc CD changer and amplifier mounted in the trunk.
It's impossible to guess how many hours went into the construction of this amazing car, but given the lengthy list of equipment that went into the build, the asking price probably doesn't even cover the components, let alone the labor. At first glance, it appears to be a basic and simple piece, but the attention to detail and amazing quality grab your attention instantly. There are very few custom cars finished to this level, and this one could undoubtedly compete at very high levels. Or take it to your local cruise night and watch as they simply hand you the trophy as you pull in—there's no hiding how brilliant this car is. Fully sorted and ready to show or drive, this is a once-in-a-lifetime car. Call now!
- Year
- 1966
- Make
- Chevrolet
- Secondary Make
- n/a
- Model Name
- Chevelle
- Secondary Model
- 300
- Vehicle Type
- Passenger Car
- Hobby Segment
- Resto-Mod
- Mileage
- 28
Engine / Transmission
- Engine Type
- Gasoline
- Engine Size
- Engine Number
- n/a
- Heads
- n/a
- Fuel Specification
- n/a
- Fuel Delivery System Type
- Single 4 Barrel
- Transmission Type
- 5 Speed Manual
- Transmission Spec
- n/a
- Transmission Number
- n/a
- Entertainment System Type
- n/a
- Battery Location
- n/a
- Battery Shut Off
- n/a
- Battery Charger
- n/a
- Power Steering
- No
- Air Conditioning
- No
- Interior Color
- Black
- Seating Type
- Bucket
- Seat Material
- Leather
- Shifter Type
- Floor
- Center Console
- No
- Body Style
- Hardtop
- Doors
- 2
- Safe Body Color
- Hugger Orange
- Paint Type
- n/a
- Stripes
- No
- Front Suspension Type
- n/a
- Rear Suspension Type
- n/a
- Axle Specification
- Ford 9" 3.50
- Front Wheel Specification
- n/a
- Rear Wheel Specification
- n/a
- Front Tire Specification
- n/a
- Rear Tire Specification
- n/a
- Front Brakes Specification
- n/a
- Rear Brakes Specification
- n/a
- Spare
- n/a
- Exhaust Type
- n/a
- Muffler Type
- n/a
- Restoration Status
- Restored
- Mileage Since Restoration
- n/a
- Awards Summary
- n/a
- Historical Documents Summary
- n/a
Contact Us Regarding this Vehicle
Call Us
- 704.445.9105
- 704.596.5980

subset: commoncrawl


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Videos - Full Browser Width Highlights
Display an HTML5 video across the entire width of your desktop website. This widget allows you to add multiple videos to your site, giving you flexibility and freedom. With this widget applied at 100% width, video will display adaptively at full browser width, regardless of screen size. Pin it, layer it behind other elements, or simply create multiple instances on a single long page.
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Discussion in 'XenForo Questions and Support' started by Paradice, Aug 2, 2011.
And get it coded, compared to vBulletin.
Edit: In all seriousness, It really depends on how in depth the skin needing coded is. I mean coding is coding and doing it for other software is almost a copy & paste. XF has a powerful style property system that can do things without even touching a template. With vB I always find myself in the templates or adding things to the additional.css template.
Your going to get 1 answer here I think and rightfully so.
What are the main differences to look out for, etc.
It's hard to say, there 2 completely different systems... there is a learning curve for XenForo but most catch on quick.
XenForo provides something the average user can hop in and create a brand new style easily.
Why don't you check the admin demo and find out for yourself:
Erm..Well if your coming from vB there is a learning curve which is to be expected. I edited my post above...which gives you my view on it.
@Russ, I checked the demo and purchased a license, and I'm trying to find a designer within the community I'm doing it for but nobody can so far. They think it's too hard or whatnot.
As for the style editor, looks too complicated for me. That's why I pay others lol.
Changing colors is easy:
Changing title image is easy:
Some other changes like layout changes require getting into the CSS code for which there is a style properties interface. You can also edit the CSS and HTML templates directly, but of course that requires coding ability:
If you want something elaborate then you need a developer. But the people on this forum can help with small tweaks if you have specific style questions.
Separate names with a comma.


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Board member
Paul Arthur is Professor of Digital Humanities at the University of Western Sydney, where he leads the UWS Digital Humanities Research Group. He was previously Deputy Director of the ANU Centre for European Studies, a joint-funded special initiative of the European Commission and the Australian National University. From 2010-13 he was Deputy Director of the National Centre of Biography, ANU, and Deputy General Editor of the Australian Dictionary of Biography. A Fellow of the Royal Historical Society, he has over fifty publications in fields of history, literature, communication and cultural studies, and has held fellowships in Australia, Europe and North America. Paul Arthur is inaugural President of the Australasian Association for Digital Humanities, and a member of the steering committee of the Alliance of Digital Humanities Organizations and the executive council of centerNet, the international network of digital humanities centres. He also serves on the executive committee of the International Auto/Biography Association and the advisory board of the Australasian Consortium of Humanities Research Centres.


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September 11, 2008
I definitely liked the live VMA version of this song better. But here's the CD quality version. This T-Pain shit is really starting to go overboard. T-Pain must be pissed as hell that his style has been taken by nearly everyone in the industry. I hope this is some kind of album cut that'll be lost and forgotten, and he'll have an album of fresh material to make up for this bullshit. Here you go:
Kanye West – Love Lockdown


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Actress Courteney Cox has ended her engagement with rocker Johnny McDaid, whom she was engaged to for about 18 months. According to a website, the former Friends star broke up with the Snow Patrol member shortly before Thanksgiving.
Courteney Cox and Johnny McDaid
The two got engaged in June 2014, after dating for six months, with McDaid proposing with the help of Cox's daughter Coco.
The couple was last seen indulging in public display of affection in September. On Twitter, the Cougar Town star shared a photograph in which they are seen embracing
each other.
Buzz suggests that the couple's relationship was put under strain by different approaches to fame, with Cox preferring to go out with friends while McDaid liked to avoid publicity.
A source said that the couple was going to counselling because they were having issues and fighting a lot, about everything.
It would have been the first marriage for McDaid and the second for Cox.
The actress was previously married to actor David Arquette. They divorced in 2013.
Meanwhile, Cox, dressed casually in a pair of jeans and a loose T-shirt, was spotted having replaced her engagement ring with a gold band.


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LONDON (Dow Jones Newswires), May 26, 2009
Norway's biggest gas field, Troll, and the Kvitebjoern and Visund gas fields have all been closed for planned maintenance, a spokesman for operator StatoilHydro ASA (STO) told Dow Jones Newswires Tuesday.
Geir Gjervan declined to give any information on how long the fields would be shut.
"This is an organized shutdown that was planned more than a year ago and was contained in our production guidance," he said.
A person familiar with the matter told Dow Jones Newswires Wednesday that Troll A would be closed for maintenance from May 25 to June 5.
The field in the northern part of the North Sea about 65 kilometers offshore Kollsnes, produced 9.36 billion cubic meters of gas in the period January to March 2009 inclusive, figures on the Web site of the Norwegian Petroleum Directorate show.
It is also a substantial oil producer, but oil is produced via Troll B and C units. Estimated average daily output of oil in 2008 was 138,000 barrels a day.
StatoilHydro owns just over 30% of Troll, Royal Dutch Shell PLC (RDSB) owns 8.10%, ConocoPhillips (COP) 1.62%, Total SA (TOT) 3.69% and state-owned Petoro 56%.
The news coincides with a planned 12 day shut down at the Kollsnes gas processing plant, which can process up to 143 million cubic meters a day of gas and some natural gas liquids and condensate volumes, and takes volumes from all three of StatoilHydro's fields.
Values for prompt U.K. natural gas however were broadly lower Monday, within-day was priced at 19 pence per therm while day ahead was pegged at 23p/therm and week ahead at 24.25p/therm, traders said.
"The system looks well balanced today, the market had priced in scheduled maintenance at these sites already," said a trader at a U.K utility.
Warm weather has trimmed demand for gas and kept downward pressure on prices in recent days. The National Grid Web site showed the system was well supplied in the afternoon, with additional supply from mid-range storage facilities.
Copyright (c) 2009 Dow Jones & Company, Inc.
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Long story short, I swapped monitors around in my home and got 3 Identical monitors setup.
1 is connected via VGA (Mobo Video)
2 & 3 are connected via DVI PCI-Express card.
Now I've been using a Dual monitor setup for a long time and I figured a 3rd monitor would be even better and actually I'm finding that its not.
So what does this have to do with Gaming?
Well, I used softTH (Software Triple Head) and got Guild Wars working across all 3 monitors, it looked cool for all about 30 seconds and than it becomes apparent that it just wasn't good visually. Maybe GW is the wrong game to test, but it was too much visual info spread across too many monitors.
Even in Windows I'm finding my 3rd monitor is sitting there wasted doing nothing, so I turn it off.
7 Replies
Oct 25, 2010 at 9:04 UTC
Triple monitors is great, you will get the most out of them if they are used at work. To date, I don't know any game that supports triple monitors.
Why woudl you want to stretch the image that far?
Oct 25, 2010 at 9:08 UTC
I love dual monitors for gaming, but I keep the game only on my main (22 in. widescreen) screen, and keep Chrome, Vent, WMP open in the second screen for reference and what not. Three screens just seems excessive.
At work I used to use dual screens, but I found I work much better without the second one.
Oct 25, 2010 at 9:21 UTC
@Elgin8949: I use dual monitors at work and I cant go back to single setup. I have a work flow going and it saves me time from tabbing to switch between applications, or comparing data from one application to another.
Now while playing the game, it doesnt stretch but rather treats all 3 monitors as one big monitor. But it just doesnt work for something like GW, maybe it might work better for a flight sim or a car racing game, or maybe a first person shoot.
@Steve Lippert: I've been doing that for GW. I keep the game in windowed mode in one window and have a Wiki Open in another window for reference.
But Tripple monitor feels excessive for me, and I've some people setup anywhere from 6 monitor to 16 Monitor setup. :)
Oct 27, 2010 at 8:00 UTC
Most of the time, from what I've seen, you really need 3 monitors that have comparable vid cards. I would love using a 3rd monitor at home, I just don't have the space.
Oct 27, 2010 at 10:24 UTC
I have duel's at work but at home i just have one nice big one. Eh i wouldn't mind two but i would hate to fight all my games about how they need to load.
Oct 27, 2010 at 2:19 UTC
I think I'm going to go back to dual monitor setup at home, I'm not using the 3rd one at all.
Regarding games. If you use 2 monitors, with most games what happens is, your character ends up in the middle of the two monitors and looks bad. So you need 3 monitors but most games dont play well on 3 monitors.
Oct 28, 2010 at 6:36 UTC
I guess 3 monitors could be useful with airplane sims and car racing games such as Burnout.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
UK's Competition and Markets Authority (CMA) has dealt a major blow to BT's much celebrated acquisition of mobile telecom operator EE with a view to enter the burgeoning quad-play market.
As reported by the Financial Times, the CMA has raised several doubts over BT's acquisition of EE which could inflict a 'potential impact' over the much anticipated deal.
The CMA is also in the process of inviting rival telecom firms to voice their opinion on the deal, a move that will surely have operators like Three, O2 and Vodafone sharpening their knives. BT's acquisition could lead to an instant dominance in landline, broadband, TV and mobile markets with a long term impact on the turnover of rival operators.
BT provides connections between mobile masts to mobile operators and rivals fear that BT's entry in the mobile space could lead to the latter allocating superior broadcast towers to itself.
Vodafone has been quite vocal in this regard by going to the extent of approaching Ofcom on BT's business practices.
However, BT seems far from being ruffled by rival concerns, claiming to be 'pleased' with the process of rival companies being offered a voice regarding the deal.
"We believe the proposed acquisition will be positive for consumers, businesses and the UK, with BT creating a world-class digital infrastructure for Britain," a BT spokesperson added.
With broadband and mobile genres taking over from the landline business in the UK at a pace faster than ever, BT's move seems to be perfect in terms of timing and scale. However, the regulators' take on the merger deal could soon turn out to be a game changer.
Watch this space.


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Saute Pans
Emeril 5-Quart Hard Anodized Covered Deep Saute Pan
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Ideal for sautéing, frying and searing, the high-sided Sauté Pan is also useful in cooking fresh sauces. Its also great for reducing and simmering sauces.
- Hard-Anodized aluminum is the essence of durability, practicality and low maintenance
- Non-stick interior for easy care and clean up
- Long stainless stick handle stays cool on the stovetop
- Glass lid allows food monitoring


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Idea Cargo Packers and Movers is your moving company of choice for your next relocation. The company was established in the year 2001. With everything you have to think about during your upcoming relocation, you shouldn't have to worry about the actual move itself. Their comprehensive moving services are unparalleled by any other moving companies.
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Related terms for "wind energy turbine ":
wind energy companies
Hadda International Group (HIG) is an International Eco Construction & Energy company operating in "Housing, Energy, and Water Engineering". HIG aims to be a leader in innovative and technological solutions pertaining to coveted Green developments for society as a whole. The focus is on sustainable construction combined with wind/solar energy and water supply, with the ...
based in Oostende, BELGIUM.
Torque Wind Turbine's goal is to accelerate the world's transition to renewable energy with a full range of increasingly affordable wind turbines. The Torque Wind Turbine company develops wind turbines and wind turbine components and is looking forward to cooperate with companies around the world for part manufacturing, distribution, installation and service to achieve ...
based in Eindhoven, NETHERLANDS.
Brumac Wind Energy Ltd design, supply, install and commision 5kW,10kW, 20kW, 30kW, 50kW and 100kW wind turbines systems throughout the UK.
based in Scotland, UNITED KINGDOM.
Pioneer Wind Energy Systems (`Pioneer Wind`) has a 10-year history developing, manufacturing, commissioning and servicing its advanced wind turbine designs. The company`s turbines are rated up to 1.65 megawatts of output and deliver clean and reliable energy in a variety of wind conditions and terrains. Pioneer Wind provides complete project management, systems ...
based in Granby,, QUEBEC (CANADA).
Aeolos wind turbine is a leading small wind turbines manufacturer in the world. Aeolos wind turbine was founded in Denmark in 1986. After 20 years development, Aeolos engineer team could provide various wind energy solutions for different customers. Aeolos owned seven patents on wind generator, control systems, blade design and inverter. Aeolos wind turbine has built an ...
based in London, UNITED KINGDOM.
Wind Energy IQ, a division of IQPC, is an international online community providing wind energy professionals with knowledge, information and articles in the areas of electrical, offshore, turbine technology, legal & tax regulatory and more. We are dedicated to creating a learning environment for sharing ideas, best practices and solutions within the wind energy community. ...
based in Berlin, GERMANY.
Fortis Wind Energy is a leading company in design, manufacturing, sales and commissioning of high performance stand alone and grid connected Small Wind Turbines. Our reputation is built on 35 years of experience in providing innovative solutions for the use of wind energy. More than 6000 Small Wind Turbines applications are installed all over the world. Some of our ...
based in Hoogkerk, NETHERLANDS.
Wind as an alternative non-emitting energy source will continue to established itself in the years to come. Energy production by means of wind power will become more effective. Worldwide, greatly diverse wind environments require modern, innovative technologies which bring high yields. Throughout the entire value-added chain of wind energy, the cost and the yield of ...
based in Rostock, GERMANY.
The German Wind Energy Association (BWE) has about 7272 individual and corporate members. This makes the BWE the largest individual association for renewable energies in Germany. The BWE represents the interests of wind turbine operators and all individuals who support wind energy.
When dealing with WEP you can relax in the knowledge that you are talking to people with a strong track record of delivering quality product and excellent service to customers over the past 25Years. It has always been our belief that providing an honest appraisal to customers is critical to gaining the confidence of the client and developing a strong vibrant business. ...
based in Co. Laois, IRELAND.
Wind Energy Solutions BV (WES) is the manufacturer of the WES50, WES80, WES100 and WES250 midsize wind turbines. WES turbines are accessible and affordable, and are distributed around the world through a global network of trained and certified partners. WES turbines are ideally suitable for distributed wind power applications. WES wind turbines provide users with reduced ...
based in Opmeer, NETHERLANDS.
Sun n Wind Energy Systems company design,manufacture,research & development,install and commissioning of 1kw to 50kw Small Wind Turbines across Asia.
based in HongKong, CHINA.
XZERES Wind designs, manufactures, and distributes high quality distributed small wind turbines (2.4kW -10kW). XZERES Wind offers wind energy solutions. Our grid connected and off grid wind turbine systems, which consist of our 2.4kW and 10kW devices and related equipment, are utilized for electrical power generation for applications and markets such as residential, ...
based in Wilsonville, OREGON (USA).
Building Turbines, Inc. (BTI) offers a unique, patented wind turbine product that can bring the dream of clean, affordable wind energy to a reality. The turbine is mounted on a strong steel frame, it has a low profile, low maintenance needs, and creates almost no noise or vibration. The BTI design possesses these exemplary and robust structural, mechanical and electrical ...
based in Austin, TEXAS (USA).
Since 2013 Primus Windpower is manufacturer of portable wind power turbines and maker of the of Air Breeze Turbine, Air 30 Turbine & Air 40 Turbine. The Primus Wind Power AIR Breeze wind turbine is trusted by sailors around the world and recommended as the best high-energy output turbine by Yachting Monthly. Its optimized electronic controls deliver energy quietly and ...
based in Lakewood, COLORADO (USA).
NaiKun Wind Energy Group Inc. is a British Columbia-based renewable energy company with offices in Vancouver, Masset and Skidegate. Located on B.C.'s northwest coast, where the wind resource is one of the strongest and most consistent in the world, NaiKun Wind's 396MW offshore wind project would generate enough energy to provide electricity to 130,000 B.C. homes. The ...
based in Vancouver, BRITISH COLUMBIA (CANADA).
The American Wind Energy Association (AWEA) is the national trade association for the U.S. wind industry – the country's fastest growing energy industry. With thousands of wind industry members and wind policy advocates, AWEA promotes wind energy as a clean source of electricity for American consumers.
based in Washington, DISTRICT OF COLUMBIA (USA).
Argosy Wind Power is a global leader in wind turbine technology. Our permanent magnet/direct drive wind turbines provide the productivity of larger wind turbines in a much smaller package. Whatever your energy needs may be, Argosy Wind Power has a turbine that will fit into your lifestyle. From residential to industrial purposes, Argosy`s quality and innovative wind ...
based in Westerville, OHIO (USA).
The Canadian Wind Energy Association (CanWEA) is a non-profit trade association that promotes the appropriate development and application of all aspects of wind energy in Canada, including the creation of a suitable policy environment. Established in 1984, CanWEA represents the wind energy community — organizations and individuals who are directly involved in the ...
based in Ottawa, ONTARIO (CANADA).
Luxmeter is active in the field of renewable and alternative energy in the south of Chile. Solutions offered include: off-grid wind turbines, on-grid wind turbines, photovoltaic panels, hydroturbines, solar powered submersible pumps, and industrials UPs.
based in Puerto Montt - Santiago Chile, CHILE.
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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Disable date if it is already previously selected by another submitterAsked by ZRBarcinova on September 02, 2013 at 11:38 AM
Hello everyone.
I am creating my first form and I know almost nothing about this. It is a registration form to play Padel. The problem is when booking the same time.
Is there any way to detect and reject form the inscription?
Thank you very much for your kind help.
(The website is in Spanish. I'm from Barcelona, Spain)
Hello, Do you mean that if someone booked a date on your form for example say tomarrow on 9/3/2013 at about 1:00pm then if another person tried to book that date you would like to have a message displayed to reject it? Can you please confirm that this is what you are talking about? Or?
Unfortunately, This is something that we don't have a feature to specifically " Reject " certain booked dates and timeframes. So for this you would have to create this via the form's source code and either hire someone or learn the programming yourself to customize it this way. I believe we have something similar for this somewhere in our Forum where it disabled booked times. This may take some time but if I can locate it or figure out something for this then I'll update you as soon as I can. It is possible that my colleagues may have some additional ideas or suggestions as well.
Okay so I started thinking about this more and one way I thought of that you could work this out would be to use an approval Method via a Confirmation Email using an AutoResponder on your Form. This way you can either use a hidden field or a field that is hidden thus if you change the Status it will update the Submission sending a notice to those whom have booked the form and you can choose what you want to display to them.
Example Form of Two different Ways. You can see the Approval Status Field but if you clone the form you'll notice that there is a Second Way to do this via a Hidden Field. The Dropdown box however can be hidden with css if you prefer. The only downside though is that none of this is automated so this is only a manual workaround to notify your customers that the date will not work so they need to discuss with you a new date.
Example Booking Form:
Example Booking Confirmation Email:
Thank you very much for your immediate response and detailed. I can do it with html? Reject the subscription when it occupied the reservation? I want to hire paying monthly JotForm form, but I need to resolve this before.
JotForm Support
Good day! I don't think this possible with HTML Formatting or by the use of the Form Source Codes. I have tried to find for workaround, but I can't find any that would accomplish the same. I guess this can only be done in server side.
I would suggest creating a separate thread requesting such function so we can forward that to our developers. However, even if the request will be forwarded to our developers, we still can't guarantee you if that will be implemented and or if our developers will be going to add it.
Thank you.
Ok. But you give me great idea. Thank you very much for help me too much.
JotForm Support
You are welcome. It's our pleasure to help. Have a great day!


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Casper Smart Cheated On Jennifer Lopez Again, Is Grounded
Casper Smart cheated on Jennifer Lopez again, but he's got a plan to win her back. More »
Casper Smart cheated on Jennifer Lopez again, but he's got a plan to win her back. More »
Casper Smart asked JLo to marry him five times, because he doesn't understand that little boys can't marry their mommies. So precious! More »
If you've been following the Jennifer Lopez/Casper Smart fiasco this week, you're probably under the impression that he's been cheating on her with transsexual model Sofie Vissa. Except, surprise, Jennifer Lopez's publicist just confirmed to People that she dumped Casper back in April which is conveniently before all the tranny sexting started:
The… More »
If you've somehow missed it over the past few days, The Dirty has been posting messages allegedly between Jennifer Lopez's driver Casper Smart and transsexual model Sofie Vissa. And while the evidence looks pretty fucking damning – and not at all surprising – it seems way too good to be true. Except now People is… More »
Last week CBS sent a memo to Grammy producers explicitly banning puffy vaginas during the show. A memo that Jennifer Lopez apparently decided didn't apply to her because here she is looking like she has elephantiasis of the lady business. Not to mention a man named Puffy was once in her vagina, so she basically… More »
Thanks to being nominated for Argo, a movie he both directed and starred in, Ben Affleck showed up to this year's Golden Globes with his wife Jennifer Garner whom he's fathered 18 children with, so naturally Jennifer Lopez had to do something to make it look like she's not a horrible spinster who can't even… More »
For most of 2012, there have been rumors about what exactly Jennifer Lopez hired Casper Smart for and how much of that money he definitely spends on gay peep shows. Turns out his real occupation is Professional Ass Blocker because here he is in Miami with JLo making sure a towel is immediately wrapped around… More »
Posted by Photo Boy
Just over a week ago Jennifer Lopez's boyfriend, Casper Smart, disproved his surname by walking into a peep show in front of at least one person standing across the street breathlessly clicking away on a fancy image replicating machine, or "photographer" to anyone with functioning brain cells. Turns out… More »
Jennifer Lopez drags Casper Smart around so people will think she's so attractive, 24-year-old backup dancers in respectable shape would rather have sex with her than chicks under 30 without kids. (In reality, he does all the driving and pretends to buy her fancy gifts with his money that's really hers.) So call it a… More »
Posted by Photo Boy
I'm not going to sit here and pretend it's not great fun watching our commenters sling hate around in the name of that guy who died supposedly to teach us a lesson about not slinging hate around, so in the interest of that – and because what's a great… More »


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Have you noticed how we're starting to see soup deals? The season is rapidly approaching. In fact, we have a fantastic deal on Progresso Soup at Target right now. We also have a great Ragu Pasta Sauce deal too. Here are your scenarios:
At Target through 9/5:
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Get $1 cashback from SavingStar for buying three Progresso Products
Use the $0.50/1 Progresso Soup or Chili Target mobile store coupon (text SAMPLE to 827438)
and use one $1/3 Progresso Products printable coupon (preclipped) or here
Price at Register: $3.50, but submit for $1 cashback from SavingStar
NET PRICE FOR ALL: $2.50 (or 83 cents each!)
BUY FOUR RAGU PASTA SAUCE, on sale Buy Three, Get One FREE (regular price $1.79)
Use one $1/2 Ragu Pasta Sauce printable coupon (use zip 90210)
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***If your store allows a coupon on an item made free by a sale, use a second $1/2 coupon. Pay $3.37 total (84 cents each!)
Thanks Totally Target.Extreme Couponing Tip: You can usually get two prints of most internet coupons. To find them in the easiest possible way, use our preclipped links that take you right to the coupon. Then come back and click the same link to get your 2nd print. This will save you the time spent searching for the coupon.
Never miss another hot coupon, deal or freebie again! "Like" Couponaholic .NET on Facebook, Follow us on Pinterest, Follow us on Twitter, Follow us on Instagram or Subscribe to our Daily Email Newsletter!


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Yukon Men
Desperate Measures
Season 6 Episode 4
Rebuilding after the fire set everyone back. Charlie needs all his native wisdom to supply his family this winter. Pat Moore gambles big, trading a prize sled dog for a handful of goats. Stan needs James' lumber to help rebuild his cabin.
- 42 min
- Premiered 03/31/2016


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Client support: Phone: (+357) 22314160
FX & CFD trading involves significant risk
05:04 Morning tech USD/JPY
Resistance 3: Y102.60 (Jun 10 high)
Resistance 2: Y102.35-45 (high of the U.S. session on Jun 10 and Jun 18 high)
Resistance 1: Y102.20 (Jun 20 high)
Current price: Y101.93
Support 1: Y101.75-80 (Jun 19-20 lows)
Support 2: Y101.60 (Jun 12 low)
Support 3: Y101.45 (May 29 low)
|remaining time till the new event being published
Market Focus
All posted material is a marketing communication solely for informational purposes and reliance on this may lead to loss. Past performance is not a reliable indicator of future results. Please read our full disclaimer.


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Greetings, I have made changes in Group Policy several days ago and ran GPResult on a few test machines and found that none of the changes have been applied. I have verified in GPResult that the GP is the correct one, but even though the changes show up in GP, they are not reflected on the machines themselves. There was Default Domain Policy and Rster. I unlinked the Default Domain Policy so it was just the Rster policy and ran GPUpdate /force on the machines. Logged off, even rebooted and then checked, the changes were not showing up. In GpResult it does still show the Rster policy taking, but my changes aren't being reflected. The changes I had made are on the Proxy settings and Disabled "Disable changing proxy settings" The proxy settings are supposed to be 188.8.131.52 but on the client machine it is showing up 10.1.11.1 (which doesn't exist and cannot find on any GP on the domain at all) and the box is still grayed out and I cannot change the proxy settings. Attached is the GPresult /v data. Any assistance that can be provided would be greatly appreciated.
6 Replies
Dec 28, 2011 at 2:17 UTC
Internal I.T. Ltd is an IT service provider.
just to confirm you have the policies for user settings assigned to the OU of the user accounts
Dec 28, 2011 at 2:21 UTC
yes, the policies are assigned. In the attachment it shows that it is grabbing the policy, but not applying it.
Dec 28, 2011 at 2:57 UTC
Unless you've modified it, I see no reason to unlink the Default Domain Policy. Other than four changes to password policies, I've always left it alone and left it linked. At first glance it looks like you're overlooking what you configured in your Rochester GPO. This is in your gpresult.txt. Maybe you didn't actually make the change in your GPO like you thought you did.
Internet Explorer Connection
HTTP Proxy Server: 10.1.11.1:8080
Secure Proxy Server: 10.5.0.202:8080
FTP Proxy Server: 10.5.0.202:8080
Gopher Proxy Server: 10.5.0.202:8080
Socks Proxy Server: 10.5.0.202:8080
Auto Config Enable: No
Enable Proxy: Yes
Use same Proxy: Yes
You may need to change that setting in GP to the numbers you actually need.
Dec 29, 2011 at 10:41 UTC
I unlinked the default domain policy to see if that would make any difference. That said, I have checked over and over again that the Rochester group policy did have the correct information. It does, but it doesn't seem to pull over to the client machine. It is getting this 10.1.11.1 information that doesn't exist.
Dec 29, 2011 at 10:46 UTC
I have added the screenshot of AD of what it shows for the Rochester GPO. Again, I have checked every single Group Policy that is on the domain and there is nothing that is referring to 10.1.11.1 address. If it does matter there was that address over a year ago, but it is no longer in existence. I cannot quite figure this out.
Dec 29, 2011 at 2:02 UTC
Remove the disabled 'disable changing proxy settings'. As stated in Group policy, it is the same as not configuring it. Therefore it's just extra policies it needs to pull down. Also, make sure you did not make any changes to other policies that affect this policy (ie 'Disable the connections page - User Configuration\Administrative Templates\Windows Components\Internet Explorer\Internet Control Panel) or in Computer Configuration.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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A DAD has sparked fury by posting a video online of his seven-year-old girl
DRIVING his car.
Ryan Taylor, 39, filmed Trudy steering his powerful BMW and uploaded the
footage on YouTube.
The clip shows Trudy on the knee of mum Elaine Peck, 34, as she takes the
wheel on a road near their home in Fraserburgh, Aberdeenshire.
Last night oil worker Ryan defended the stunt. He said: "She loves it. My dad
did the same with me and I passed my test first time."
But road safety charity Brake said: "It's illegal and dangerous."
Neil Greig, of the Institute of Advanced Motorists, called it "totally
stupid". Cops are studying the footage.


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Profiling microRNAs in lung tissue from pigs infected with
Human and rodent genomics
MicroRNAs (miRNAs) are a class of non-protein-coding genes that play a crucial regulatory role in mammalian development and disease. Whereas a large number of miRNAs have been annotated at the structural level during the latest years, functional annotation is sparse.
In this study, the RNA extracted from visually unaffected and necrotic tissue from pigs infected with
This is the first study revealing significant differences in composition and expression profiles of miRNAs in lungs infected with a bacterial pathogen. Our results extend annotation of microRNA in pig and provide insight into the role of a number of microRNAs in regulation of bacteria induced immune and inflammatory response in porcine lung.
MicroRNAs (miRNAs) are approximately 22 nucleotide (nt) long molecules, constituting a highly abundant class of non-coding RNAs (ncRNAs) encoded in the genome of all eukaryotes
In protection against intruding pathogens, an organ-specific as well as a systemic immunological host response is activated. This response is activated by the presence of so called pathogen-associated molecular patterns (PAMPs) that are sensed by Pattern Recognition Receptors (PRRs), for instance the Toll-like receptor (TLR)
The pig (
Overview of high throughput sequencing, alignment and clustering
To investigate the composition and dynamic changes of ncRNA (miRNA in particular) expression in lung tissue from pigs infected with APP
Figure 1
The distribution of raw reads versus read lengths in both libraries
The distribution of raw reads versus read lengths in both libraries. X axis shows the insert length while Y axis represents raw reads. Necrotic area sample is marked in blue whereas visually unaffected area sample is marked in red.
Using reads that aligned uniquely to the pig genome version 9, we found 361,430 read clusters shared between the libraries (Table
NcRNA detection by homology and class-specific tools
We annotated the main classes of ncRNAs using an
Figure 2
The distribution of raw reads between various RNA classes in A) visually unaffected sample and B) necrotic sample
The distribution of raw reads between various RNA classes in A) visually unaffected sample and B) necrotic sample.
Detection of miRNAs by homology
The known miRNAs were identified by BLAST
Detection of novel miRNAs
A distinct pre-miRNA hairpin structure is a primary criterion for microRNA annotation. The hairpin structure and characteristic read patterns of miRNAs makes them much easier to detect than other novel ncRNAs in general
We found 303 miRNAs annotated by miRDeep2 where 221 were from known miRNAs and eight were from other types of ncRNAs. Overlapping the miRDeep2 results with the Novoalign read clusters of at least five uniquely mapped raw reads, we confirm 201 of the 303 miRDeep2 detected miRNAs. Among the 201 clusters found by both miRDeep2 and the clustering of the reads aligned with Novoalign, 4 predictions were also matched to non-miRNA infernal families, 160 were aligned to known miRNAs, and 37 were predicted to be new, un-annotated miRNAs.
These 37 novel predictions were further evaluated by requiring at least five reads detected by miRDeep2, and either both mature and star sequences to be present, or alternatively structure conservation of the site by RNAz pre-release version 2
Additional file 1
Profiles of read clusters for
Click here for file
Additional file 2
Profiles of read clusters for
Click here for file
Additional file 3
The known miRNAs of the miRDeep2 pipeline missed by the unique mappings from Novoalign. 24 of these miRNAs are found twice in the genome, indicating assembly errors. Raw reads are un-normalized and according to the Bowtie alignment produced by miRDeep.
Click here for file
Profiles of expression during APP infection
Characterization of the libraries composition
The ncRNAs of the necrotic tissue are dominated by the degraded rRNAs. Other long ncRNAs also show high raw read counts compared to the unaffected tissue. It may, however, be noted that the fraction of tRNAs are comparable in the two samples. The summed raw reads of the microRNAs in particular as well as snoRNAs are much lower in the necrotic sample compared to the visually unaffected one.
We annotated 9260 clusters as being parts of protein coding genes (exons). The raw reads for these clusters are quite different between the samples, 204,431 and 12,494 reads in necrotic and visually unaffected respectively, which underlines the higher degree of degradation of mRNAs in the necrotic tissue which poses challenges in the interpretation of the microRNA expression levels (Table
Highly abundant microRNAs
High throughput Illumina GAIIx sequencing revealed a number of highly expressed microRNAs. We present the 20 most abundant microRNAs in both samples (Table
After normalization of the raw reads accordingly we found that 17 of the 180 known and novel miRNAs were significantly up regulated in the necrotic sample with an e-value cutoff of 0.05. Similarly, 12 miRNAs were significantly down regulated (Table
Additional file 4
Expression of the all the detected miRNAs. Columns 6 and 7 contain the raw reads in the two samples. Columns 8 and 9 are the read counts normalized by the TMM method. Column 10 is log2 of the fold change based on the normalized read counts. Column 11 is the p-values for the relative expression based on the exact test from the edgeR package using a dispersion-factor of 0.1. (see Methods for details).
Click here for file
The top 20 most expressed snoRNAs show a much larger variation between the two samples. Nine are common in the top 20 list of most expressed snoRNAs in the two respective samples (for details see
Additional file 5
Top 20 snoRNAs in the necrotic tissue. Infernal e-values are given for the annotation.
Click here for file
Additional file 6
Top 20 snoRNAs in the unaffected tissue. Infernal e-values are given for the annotation.
Click here for file
The high throughput sequencing results were further subjected to validation of candidate miRNA expression by RT-qPCR. Most of the miRNA candidates are falling into highly abundant or up/down regulated microRNAs in the necrotic sample, however the two miRNAs: miR-15a and miR-155 were chosen due to their biological relevance as reported in the literature disregarding their rather low read count (
Figure 3
Profile of reads distribution for read cluster annotated as both miR-664-5p and SNORA36
Profile of reads distribution for read cluster annotated as both miR-664-5p and SNORA36. S1-necrotic, S2-visually unaffected sample.
qPCR validation of candidate microRNA expression
High throughput data demand validation of particular candidates by a technique with higher specificity and sensitivity like RT-qPCR. In this study, SYBR Green RT-qPCR was used to verify the expression of 13 select microRNAs and one snoRNA. Within 13 microRNAs, 12 are annotated in miRBase and one is a novel microRNA discovered in the present study. Additionally, miR-152 and miR-191 were used as RT-qPCR reference genes.
In addition to RNA isolated from visually unaffected and necrotic tissues, RNA from the demarcation zone (between infected and non-infected tissue), and from nose and trachea originating from the same infection study were included in the RT-qPCR studies. Moreover, developmental lung (gestation day 50, gestation day 100 and 3 months old ( adult control) were included in order to obtain microRNA expression profiles across tissues present in the respiratory tract as well as across developing uninfected lung tissue. The data for trachea, nose, F50, and F100 are not included in the main manuscript however the diagram including all the tissues is present in
Additional file 7
Bar diagram showing RT-qPCR results of expression of 13 selected unique miRNAs and one snoRNA. miR-d5 represents a novel unannotated microRNA. All eight sample groups included.
Click here for file
Figure 4
RT-qPCR analysis of expression of 13 selected unique miRNAs and one snoRNA
RT-qPCR analysis of expression of 13 selected unique miRNAs and one snoRNA. miR-d5 represents a novel unannotated microRNA. Data presented are normalized to the reference genes, LOG2-transformed relative quantities. The level of expression for control sample is set to zero.
The ANOVA performed on LOG2 transformed relative quantities revealed significant differences in the ncRNA expression between the analyzed tissues. As mentioned above, Illumina GAIIx high throughput sequencing was performed on pools of visually unaffected and necrotic sample only. RT-qPCR, on the other hand provides additional, complementing results for the demarcation zone as well as a control sample group. Taking the nature of the demarcation zone sample (tissue sampled at the border of necrotic and visually unaffected tissue, see Figure
Figure 5
Sampling areas of the porcine lungs infected with
Sampling areas of the porcine lungs infected with
We arranged the expression profiles of the genes selected for RT-qPCR in five groups.
Group 1: microRNAs up-regulated in all or some of the infected samples compared to control (miR-144* (miR-144-5p in the newest miRBase 18.0 in human), miR-223, miR-451, miR-664-5p, miR-d5, SNORD15), (p-values shown in
Additional file 8
Statistical analysis of qPCR results: One way ANOVA performed on four groups: control, visually unaffected, demarcation zone, necrotic.
Click here for file
Group 2: microRNAs down-regulated in necrotic comparing to visually unaffected areas and/or control sample (miR-126, miR-155). The read counts for miR-126 also showed significantly lower number of counts for the necrotic sample in comparison to the unaffected sample. MiR-126 has the highest expression in control sample, which decreases significantly with the necrosis progression. MiR-155 has a slightly different profile with all the infected samples having significantly lower expression than the control sample but at the same time the expression does not differ significantly between samples representing various stages of infection (similar read count number confirms this expression pattern).
Group 3: MicroRNAs down-regulated in control followed by up-regulation in visually unaffected and down-regulation again in necrotic sample (miR-142-5p, miR-143-3p, miR-148a). Despite the decreasing trend, the expression for miR-142-5p and miR-148a, in the necrotic sample remains significantly higher than in the control. In contrary, miR-143-3p makes a slight exception by having the expression in necrotic area down-regulated significantly compared to the control. Expression profile of miR-142-5p similarly shows lower read count number in visually unaffected vs. necrotic sample obtained by sequencing. Surprisingly the differential expression detected by RT-qPCR does not confirm the lack of significant differences for the miR-148a and miR-143 shown by RNAseq data.
Group 4: microRNA highly up-regulated in infected samples vs. control, showing no differences between the different sampled areas from infected lung (miR-15a). Among all unique qPCR candidates, miR-15a represents an exclusive profile of very low expression in the control sample, followed by remarkable up-regulation (p-value =1 E-08) in all 3 samples originating from the infection study. No significant difference of the miR-15a expression has been detected between the infected samples (visually unaffected, demarcation zone, necrotic), which is also reflected in the RNAseq results.
Group 5: Two microRNAs showed no significant difference (miR-21) between the investigated tissues or a slightly significant difference (miR-146a-5p) between control and visually unaffected area (p-value = 0,48). In contrary, the RNAseq results show up-regulation of miR-146a-5p placing it within 20 most regulated microRNAs in necrotic sample. MiR-21 is by far, the most expressed of all the assayed microRNAs, showing very high, stable expression in the lung tissue, regardless the presence/degree or absence of infection. However, according to read counts miR-21 is placed as the second most abundant transcript, right after miR-143. Furthermore, the relative expression of miR-21 detected by RNAseq points towards up-regulation of this microRNAs in visually unaffected sample in comparison to the necrotic sample (visually unaffected sample contains more than 2 times more reads than the necrotic).
Target predictions of microRNAs
Regardless of the limited understanding of microRNA function, microRNA target predictions point out mRNAs possibly regulated by individual miRNAs. We combined (conservative) human data from four of the prominent target prediction methods sources namely: Targetscan
Overlap of the mRNA and miRNA predictions resulted in 149 predicted interactions for the 91 proteins with the 12 miRNAs of which 35 interactions (corresponding to 26 distinct proteins) are predicted by two or more methods and are shown in
Additional file 9
Targets predicted for 12 select miRNAs and 91 select proteins. Only targets predicted by two or more of the four target prediction methods (see Methods for details).
Click here for file
The miRNA candidates assayed by RT-qPCR are found to target multiple mRNAs as shown in Table
The target predictions for the 11 novel miRNAs were performed with TargetScan against human 3' UTRs, keeping only the widely conserved target sites. For the novel miRNAs we found a total of 1,926 targets. Only eight out of 1,926 predicted interactions target mRNA within the selected 91 proteins and these are: CYP26B1: miR-d11, ILF3: miR-d10, MCL1: miR-d7, PAK2: miR-d3, miR-d7, miR-d8, SMAD7: miR-d12. SOCS5: miR-d7. The highly expressed novel miRNA miR-d5 was found to target only five transcripts, which is an unusual low number compared to the others. This characteristic is shared with miR-d11 and it is likely because both miRNAs are linage specific and not found in human (see
Additional file 10
Conservation of miRNAs found in this article. In column 2–5 the conservation is based on full genomic pairwise alignments of pig and 17 other mammalian genomes (bosTau4, turTru1, equCab2, felCat3, canFam2, eriEur1, hg19, tarSyr1, mm9, rn4, oryCun2, loxAfr3, echTel1, dasNov2, choHof1, monDom5, ornAna1). The miRNA pig coordinates are required to transfer to the target organism, and the coordinates in the target organism is required to transfer back to the same position in pig to avoid paralogous alignments. The pig sequence is then aligned to the target sequence and a minimum align length of 50 and an identity of at least 50% is required for the miRNA to be counted in the third column. In columns 2 and 3 the pairwise identity between the pig sequence and cow or human is given, and in column 4 the number of organisms where the miRNA is found is given and finally in column 5 the mean pairwise identity is given. Columns 6–9 are analogous to column 2–5, except the identification of the miRNAs in the target organism are now identified by a BLAST of mirBase version 18 against the target genome. The pig and target sequence are then aligned in the same way as before.
Click here for file
Additional file 11
Targets predicted with TargetScan for mir-d5 and mir-d11 for which the set of conserved target sites gave few results. The targets are predicted for 3' UTRs from cow since the pig ones are not in the dataset for TargetScan (see Methods for details).
Click here for file
In the present study we have mainly been interested in investigation of microRNAs involved in the progression of the APP infection rather than comparing infected versus healthy individuals. Furthermore, we decided to use infected and visually uninfected tissue from pigs infected with the bacterial pathogen
The sequencing data provide evidence of microRNA deregulation during lung infection with the bacterial pathogen. Both up- and down-regulation of microRNAs were found when comparing the expression in the necrotic versus visually unaffected tissue of the lung. A subset of the highly expressed and biologically relevant genes (13 miRNAs and one snoRNA) was subjected to RT-qPCR. In 10 out of 14 genes, RT-qPCR results supported the results obtained by deep-sequencing indicating that the data generated reflects a biologically meaningful profile. The disagreement featured in the remaining four microRNAs is most likely pointing towards sequencing bias accompanying RNAseq experiments rather than misevaluation of the expression by a specific and sensitive RT-qPCR.
The number of annotated microRNAs in domestic pig in the newest miRBase 18.0 is still much smaller than in other organisms, featuring 1527 entries for human, 741 for mouse and only 228 for pig. Multiple alignments to the pig genome and 17 other organisms were performed based on the assumption that the majority of miRNA sequences are conserved among species
Studies on high throughput sequencing of small RNAs show that there are in most cases a few distinct microRNAs that constitute the majority of microRNA reads
The second most abundant microRNA in both samples – miR-21 is involved in many biological scenarios
The 12 microRNA candidates assayed by RT-qPCR were investigated for target predictions in order to provide more insight into the biological pathways where the different microRNAs could play a role. The conservative predictions indeed anticipated a number of very promising, biologically interesting interactions. One of the major factors involved in detection of pathogens and initiation of inflammatory response are TLRs. Moreover, a rapid immune response is triggered upon pathogen invasion by the presence of LPSs – a major component of the gram negative APP membranes. After binding of LPS to the TLR4/MD2/CD14 receptor complex, signalling pathways lead to the activation of nuclear factor κB (NF-κB) that further regulates a large set of genes critical to cell proliferation, inflammation and innate immunity
Two targets of miR-21: Interleukin I Beta (IL1β) and tumor necrosis factor, alpha-induced protein 3 (TNFAIP3), which is rapidly induced by the TNF were assayed by RT-qPCR on mRNA in another APP infection study in pigs
The following key player in control of immunity and inflammation, miR-155, showed rather contradicting results to those existing in the literature
The 5b APP serotype used in the present study is highly virulent. It has been show that the lethality of pleuropneumonia in pigs is not necessarily caused by ventilator failure
Another microRNA candidate namely miR-223 was investigated in the present study. We indeed detected a remarkable, gradually increasing expression of miR-223 with infection progression, in infected samples in comparison to the control. Moreover, Matsushima and co-workers (2011) found miR-223 to be the only up-regulated microRNA in
Nearly all up-regulated microRNAs in lung tissue from infected pigs target IL-6R and/or IL6ST, in addition miR-144 and miR-146a 5p targets mRNA coding for IL-6. IL-6 was found to be highly up-regulated in the same animals
This is to our knowledge the first study to survey the host microRNA expression profiles in
Experimental infection
Ten, 8–10 weeks old castrates, Danish crosses between Landrace/Yorkshire/Duroc, free from
RNA extraction
Total RNA was extracted from each necrotic lung, visually unaffected lung, demarcation zone lung, trachea, nose, F50, F100 and control porcine sample, using Tri Reagent® (Molecular Research Center, Inc., USA) following the manufacturers recommendations. App. 300 mg of each tissue was used for the isolation procedure. RNA quantity was determined on a Nanodrop 1000 (Peqlab Biotechnologie, Germany). Additionally, the integrity of the total RNA samples was measured by total RNA Assay and Experion RNA StdSens Analysis Kit using 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and Experion (Bio-Rad Laboratories, Inc., Hercules, CA, USA) respectively. The RNA concentrations assessed by Nanodrop as well as the integrity values of RIN and RQI are provided in the
Additional file 12
Table listing values describing the quantity and integrity of all RNA samples.
Click here for file
Small RNA library and Solexa sequencing
Total RNA fractions from eight necrotic and ten visually unaffected samples were pooled, respectively and used for the small RNA library construction following standard Illumina protocols version 1.5. Briefly, RNAs were ligated to a 5' and a 3' adapter sequentially, reverse transcribed into cDNA, PCR amplified with adapter specific primers, and finally the small RNAs were purified on 3% MetaPhor® Agarose gel (Cambrex Bio Science Rockland, USA) to generate suitable length of tags for small RNA sequencing performed on Illumina Genome Analyzer IIx (Illumina GAIIx).
Computational analysis of high throughput sequencing data
Alignment and clustering of reads
The reads were filtered for chastity by the Illumina pipeline, after which they were aligned to version 9.0 of the pig genome using Novoalign version 2.05.25. The reads were stripped of adapter allowing for up to two mismatches, and minimum insert lengths of 18 nucleotides were required prior to alignment. Novoalign's microRNA scoring scheme was employed. All alignments, no more than five points away from the best alignment are reported by Novoalign. Novoalign filters for quality, strips adapter sequence, and filters out homo-polymer reads prior to aligning to the genome. Only the aligned reads mapping uniquely were used for read clustering. The reads in each library were clustered allowing for a gap of up to 15 uncovered nucleotides before breaking a cluster. Clusters were then merged between the two libraries to get one consistent set of clusters.
The read clusters were annotated using a number of methods: The Ensembl protein annotation for build 9.0 of porcine genome; the high confidence BLAST (95% id, 95% length)
Cross species conservation and novel ncRNAs
A UCSC (The University of California, Santa Cruz) genome browser style multiple alignment based on pig and 17 other vertebrates was performed using lastz
Search for novel miRNAs
Novel miRNAs were explored using the miRDeep2 pipeline
In this experiment the libraries and raw reads could not be compared directly due to large variation in the fractions of degraded RNAs. We therefore chose to treat the miRNAs from each sample separately, which leads to summed miRNA raw reads of 791541 and 4672073, respectively for the 180 miRNAs detected in the samples. When the samples as here are very diverse it is further recommended to calculate normalization factor by the threaded means of M-values (TMM) method
Target predictions
We used miRNA/protein target data for human from the following sources TargetScan
cDNA synthesis for RT-qPCR
Individual samples from all animals mentioned in the experimental infection section above were subjected to RT-qPCR study. The integrity of the total RNA ranged from 6.2-8.7. For more details see
This miR-specific qPCR method as previously described in
Of the miRNAs investigated in the present study, miR15a, miR21, miR126, miR142-5p, miR144-5p, miR146a-5p, miR148a, miR152, miR155, miR192, miR-223, miR-45 and miR-d5 are 5'-miRNAs hence only the mature miRNAs of these targets were detected. For miR-143-3p, which is a 3'-miRNA both the mature miRNA and the pre-miRNA were detected.
Quantitative RT-PCR of microRNAs
Fourteen candidate genes (13 microRNAs and 1 snoRNA) and two reference genes (miR-152 and MiR-191) were assayed by RT-qPCR. Primer sequences for each assayed ncRNA gene (including reference genes) are listed in the
Additional file 13
Table listing RT-qPCR primer sequences for each assayed miRNA and snoRNA. * indicates reference genes.
Click here for file
RT-qPCR data analysis
PCR efficiency was calculated from the log-linear portion of the standard curves
Additional file 14
Statistical analysis of qPCR results. One way ANOVA performed on eight groups: trachea, nose, F50, F100, control, visually unaffected, demarcation zone, necrotic.
Click here for file
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
Conceived and designed the experiments: AP, SC, MF. Performed the experiments: AP. High throughput sequencing: MB, NT, AP. Conceived and designed the bioinformatics analysis: CA, JG, AP, SC, MF. Analysed the data: AP, SC, MF, CA, JG, KS. Contributed reagents/materials/analysis tools: SC, KS. Wrote the paper: AP, SC, MF, CA, JG, KS, PMHH. All authors read and approved the final manuscript.
We thank Peter K. Busk for RT-qPCR primer design. This work was supported by grant from High Technology Foundation and PhD grant from Faculty of Life Sciences, KU to AP. The bioinformatics work was supported by the Danish Council for Independent Research (Technology and Production Sciences), The Danish Council for Strategic Research (Programme Commission on Strategic Growth Technologies), as well as the Danish Center for Scientific Computing.
MicroRNAs: genomics, biogenesis, mechanism, and function
New microRNAs from mouse and human
Vertebrate microRNA genes
The functions of animal microRNAs
MicroRNAs in development and disease
Involvement of microRNAs in physiological and pathological processes in the lung
Toll receptors in innate immunity
Septic shock; current pathogenetic concepts from a clinical perspective
Antiviral immunity directed by small RNAs
The role of RNAi and microRNAs in animal virus replication and antiviral immunity
A cellular microRNA mediates antiviral defense in human cells
Cellular microRNA and P bodies modulate host-HIV-1 interactions
Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA
MicroRNAs: new regulators of immune cell development and function
The insulin-like growth factor system and its receptors: A potential novel anticancer target
NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses
Modulation of miR-155 and miR-125b levels following lipopolysaccharide/TNF-alpha stimulation and their possible roles in regulating the response to endotoxin shock
MicroRNA-155 modulates the interleukin-1 signaling pathway in activated human monocyte-derived dendritic cells
Induction of microRNA-155 during Helicobacter pylori infection and its negative regulatory role in the inflammatory response
Analysis of the host microRNA response to Salmonella uncovers the control of major cytokines by the let-7 family
Actinobacillus species and their role in animal disease
Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection
Transcriptional profiling at different sites in lungs of pigs during acute bacterial respiratory infection
Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags
Pigs in sequence space: a 0.66X coverage pig genome survey based on shotgun sequencing
miRBase: integrating microRNA annotation and deep-sequencing data
miRBase: tools for microRNA genomics
miRBase: microRNA sequences, targets and gene nomenclature
The microRNA Registry
Small RNA discovery and characterisation in eukaryotes using high-throughput approaches
Ensembl 2012
Basic local alignment search tool
Infernal 1.0: inference of RNA alignments
Rfam: updates to the RNA families database
De novo prediction of structured RNAs from genomic sequences
From structure prediction to genomic screens for novel non-coding RNAs
Discovering microRNAs from deep sequencing data using miRDeep
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets
MicroRNA targeting specificity in mammals: determinants beyond seed pairing
Most mammalian mRNAs are conserved targets of microRNAs
A genome-wide map of conserved microRNA targets in C. elegans
Combinatorial microRNA target predictions
Human MicroRNA targets
MicroRNA targets in Drosophila
The microRNA.org resource: targets and expression
Comprehensive modeling of microRNA targets predicts functional non-conserved and non-canonical sites
Accurate microRNA target prediction correlates with protein repression levels
Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene
Wheat hybridization and polyploidization results in deregulation of small RNAs
High throughput sequencing of microRNAs in chicken somites
Analysis of microRNA transcriptome by deep sequencing of small RNA libraries of peripheral blood
Lung microRNA: from development to disease
MicroRNAs in idiopathic pulmonary fibrosis
MiRNA in innate immune responses: novel players in wound inflammation
MicroRNAs in NF-kappaB signaling
A trio of microRNAs that control Toll-like receptor signalling
NF-kappaB: linking inflammation and immunity to cancer development and progression
Regulation of neutrophil senescence by microRNAs
MicroRNA-21 inhibits toll-like receptor 2 agonist-induced lung inflammation in mice
MicroRNA signatures in Helicobacter pylori-infected gastric mucosa
Negative regulation of TLR4 via targeting of the proinflammatory tumor suppressor PDCD4 by the microRNA miR-21
Co-expression of host and viral microRNAs in porcine dendritic cells infected by the pseudorabies virus
Requirement of bic/microRNA-155 for normal immune function
MicroRNA-155 is induced during the macrophage inflammatory response
Blood gas and hematological changes in experimental peracute porcine pleuropneumonia
Allele-specific targeting of microRNAs to HLA-G and risk of asthma
Altered expression of microRNA in the airway wall in chronic asthma: miR-126 as a potential therapeutic target
Hepatic gene expression changes in pigs experimentally infected with the lung pathogen Actinobacillus pleuropneumoniae as analysed with an innate immunity focused microarray
snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs
tRNAdb 2009: compilation of tRNA sequences and tRNA genes
miRBase: tools for microRNA genomics
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence
Aligning multiple genomic sequences with the threaded blockset aligner
Human miRNA precursors with box H/ACA snoRNA features
Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells
The Reactome BioMart
Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments


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Applicable robotic systems for forging die repair have required a considerable investment in preparation time and materials, adding to the overall cost of the project. NeWeld has combined several automation and welding technologies to introduce the first die-welding robot package.
Absolute Welding and Machining has been in the business of die reclamation and service-life extension for the past 20 years (originally as Absolute Welding), and working closely with welding-wire suppliers like Eureka Welding Alloys to expand the capabilities and applications of welding alloys. Over these two decades there has been steady progress in the development of welding alloys, but the application of automation technology — i.e., robots — for die repair has been ...
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A Revolution in
Dinner Partying
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BSF Admit Card 2015
BSF Admit Card 2015 is about to offered by Border Security Force to the candidates who have applied for the BSF Constable Exam 2015. You can download the BSF hall ticket online with the help of this page as we the team of bharatrecruitment.com is providing you some simple steps for getting it from the official website.
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Organizer's Name:
Border Security Force (BSF) is a Border protector power of India. Recognized on December 1, 1965, it is a paramilitary power charged with protecting India's land border through calm time and preventing transnational misdeed.
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by VALERIO BERDINI
About the Book
"Is there a way to portray Venice, during the Carnival, including the historic appeal of its colourful masks without denying it is happening in a new millennium?"
This book is a challenge. I dropped my favourite black and white film to opt for a contemporary medium, digital colour photography, with the purpose of putting a secular tradition in the contemporary setting where it is taking place today.
28 exclusive images
Texts in English and Italian
About the Creator
Born in Rome, Italy, in 1970, Valerio Berdini has been living in the UK since 2001. Over the years his documentary work developed into a personal language that merges a traditional aesthetic, influenced by the masters of photojournalism, with an incessant attention to the evolution of the contemporary society. He has travelled since very young and the desire to meet new people, in their own culture, never stopped. Music fan and avid concert photographer, he has also built an extensive archive of several hundreds live rock bands. Valerio Berdini photographs have been exhibited, awarded and published internationally, including The Guardian, La Repubblica, Condé Nast Traveler, Rolling Stone, L'Espresso, The Times and many more. He is a freelance photographer represented by REX Features.
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Central High's Raegan Willis enjoyed a record-setting night at the Allstate Sugar Bowl/LHSAA Division I state powerlifting meet Saturday at Alexandria Senior High.
Willis won the 165-pound weight class and was the outstanding lifter on the upper weight-class platform. Two of her three lifts established state records in the 165 weight class and the other tied a record.
A total lift of 1,030 pounds was only part of the story for Willis, who set state records with a 400-pound squat and a 430-pound deadlift. She tied a record with a 200-pound bench press.
Another Central girl, Reagan Cress, won the 148-pound class with a total lift of 860 pounds that included a 340-pound squat. Walker High's Breanna Gurzynski won the girls 220-pound plus division with a total lift of 1,085 pounds, including a 520-pound squat.
Central also had boys champion as Blaise Courville won the super heavyweight class. Courville's total lift of 1,690 pounds that included a 700-pound squat.
Results to stand
LHSAA Assistant Executive Director Keith Alexander said the final results for the girls Division V powerlifting meet held Saturday at Pope John Paul II will stand as previously reported. Alexander confirmed that a question about the final team totals was raised.
Delhi and Ascension Catholic tied with 45 points, just two ahead of False River Academy. Delhi claimed the team title by virtue of having more first-place finishers. Alexander said powerlifting officials were in on the process that ended with the decision that the results would stand as is.
"Things are going to stay the way they are in the team standings," Alexander said. "There were some issues at the meet. But ultimately, those results won't change."
Broadmoor seeks coach
Broadmoor High seeks a head girls basketball coach for 2015-16. Applicants must be certified to teach in East Baton Rouge Parish.
BHS also seeks a spring football opponent. Contact Broadmoor Athletic Director Rusty Price at (225) 924-7900.


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Cybex Behavior Change and Exercise Adherence
YMCA of Silicon Valley - Training Department
Thursday, August 21, 2014 from 10:00 AM to 2:00 PM (PDT)
Event Details
Cybex Behavior Change and Exercise Adherence
Exercise starts, continues and ends for people for many different reasons. A popular area of study is Behavior Change and how utilizing both modern and traditional models can help the exercise provider understand how to encourage exercise in their client, regardless of their stage of readiness. This workshop examines common theories of Behavior Change and looks at hard evidence on using these models of changes in everyday practice.
Attendees will understand general common themes in theories of behavior change, with a focus on the Transtheoretical Model of the Stages of Change, and what implications those theories may have on starting exercise and exercise adherence. Attendees will show their ability to identify the stage of change and alter coaching methods through the use of Case Studies and discussion.
Thursday, August 21st
10AM-2PM (will have a working lunch at approximately 12:30PM)
El Camino YMCA - 2400 Grant Rd. - Mt. View, CA
Kiwanis Room
Heather Coppock brings a wealth of knowledge on the physiology of sport to her
role at the Cybex Research Institute. As an Instructor at Salem State University, she teaches classes in the Sport and Movement Science Department including Exercise Physiology, Strength and Conditioning, Strength and Conditioning Techniques, Fundamentals of Exercise, Nutrition for Sport and Physical Performance, Advanced Resistance Training, and directed field study. Heather earned her BA in Athletic Training from Northeastern University and graduated with honors from Appalachian State University with a Masters in Exercise Science. She is a strong advocate for integrating the science and practice of exercise to make it an integral part of the healthcare continuum.
1 ACSM credit per hour (4 hour class) = 4.0 credits
Target Audience:
Health & Wellness Leadership Staff, Wellness Coaches, Personal Trainers & Front-line Wellness staff
When & Where
El Camino YMCA
2400 Grant Rd
Mountain View,
Thursday, August 21, 2014 from 10:00 AM to 2:00 PM (PDT)
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