text:
Effects of vaccination against paratuberculosis on tuberculosis in goats: diagnostic interferences and cross-protection
Bacteriology, parasitology and mycology
Most countries carrying out campaigns of bovine tuberculosis (TB) eradication impose a ban on the use of mycobacterial vaccines in cattle. However, vaccination against paratuberculosis (PTB) in goats is often allowed even when its effect on TB diagnosis has not been fully evaluated. To address this issue, goat kids previously vaccinated against PTB were experimentally infected with TB.
Evaluation of interferon-γ (IFN-γ) secretion induced by avian and bovine tuberculins (PPD) showed a predominant avian PPD-biased response in the vaccinated group from week 4 post-vaccination onward. Although 60% of the animals were bovine reactors at week 14, avian PPD-biased responses returned at week 16. After challenge with
Our results suggest a degree of interference of PTB vaccination with current TB diagnostics that can be fully mitigated when using new DIVA reagents. A partial protective effect associated with vaccination was also observed in some vaccinated animals.
Caprine tuberculosis (TB), caused either by
Control of PTB in small ruminants can be facilitated by vaccination because the bacterial burden is greatly reduced, containing the spread of the disease and preventing clinical expression
On the other hand, it has been shown that exposure to
It has been reported that TB diagnostic tests currently used in cattle, such as the skin test or the interferon-gamma (IFN-γ) assay, can be used for diagnosis of TB in goats
The aim of the present work was to assess how vaccination against
Assessment of diagnostic tests
The effect of
The tuberculin-based IFN-γ test was performed every 2 weeks throughout the experiment. The kinetics of IFN-γ responses of the vaccinated group showed a PPD-A-biased response, which switched to a PPD-B-biased mean response 4 weeks after
Figure 1
Kinetics of IFN-γ released after stimulation of whole blood with tuberculins in the vaccinated group.
Kinetics of IFN-γ released after stimulation of whole blood with tuberculins in the vaccinated group. Avian tuberculin (PPD-A, ▲), bovine tuberculin (PPD-B, ■). Results are expressed as mean ΔOD450 (mean of OD450 of antigen-stimulated values with unstimulated values subtracted) ± 95% confidence interval.
Next, we assessed the interference of
Seropositivity to
Pathology and bacteriology
At necropsy on week 28 (14 wpi), visible pathology typical of TB was observed in the lungs and pulmonary lymph nodes (LN) of all animals irrespective of their vaccination status. Of the seven lung lobes evaluated, the vaccinated group presented an average of three lobes with gross lesions (2–4, 95% CI), slightly lower than the mean of four lobes (3–5, 95% CI) found in the unvaccinated group, but this difference was not statistically significant (
The mean log10-transformed volume of gross lesions in lungs was similar in the unvaccinated (1.3 log10 cm3; 1–1.7, 95% CI) and vaccinated (1.1 log10 cm3; 0.5–1.6, 95% CI) groups (
Figure 2
Post-mortem analysis as measured by gross pathology and bacterial burden.
Post-mortem analysis as measured by gross pathology and bacterial burden. Results are plotted for individual goats. (A) Total volume of visible lesions (VL) in lungs as log10 cm3. (B) Total volume of VL in respiratory lymph nodes (LN) as log10 mm3. (C) Bacterial load as log10 cfu/g. (●) vaccinated goats, (■) unvaccinated goats. Horizontal lines indicate median values. Significance determined by unpaired
In addition, none of the vaccinated goats showed extrapulmonary gross lesions, whereas dissemination was observed in 4 of 10 unvaccinated goats that presented with gross lesions beyond the thoracic area (2 goats with lesions in retropharyngeal LN, 2 goats with lesions in mesenteric LN, and 1 goat with lesions in the spleen). These lesions were histopathologically confirmed to be tuberculous.
In three animals, focal lesions were also found in the LN draining the
Finally, the mycobacterial load in LN was calculated as log10 cfu/g. The bacterial burden in the pulmonary and retropharyngeal LN of all animals (n = 20) ranged from 2 to 4 log10 cfu/g (Figure
Cross-sectional analysis
To evaluate the vaccine effect in terms of heterologous protection, post-mortem parameters were also assessed in combination. A mild positive correlation between bacteriological and pathological findings was found by correlating individual logarithmic values of bacterial load and total volume of visible lesions (r = 0.386,
Figure 3
Protective efficacy measured by post-mortem results, and cross-sectional comparison of IFN-γ results with vaccination outcome.
Protective efficacy measured by post-mortem results, and cross-sectional comparison of IFN-γ results with vaccination outcome. (A) Correlation between pathological and bacteriological parameters of all goats (n = 20). Dashed line (y = −x + 4.16) indicates the threshold defined to divide vaccine responders (VR) and vaccine non-responders (VNR). Symbols represent vaccine cross-protection outcome: (■) unvaccinated controls (UC), (○) VR goats, (●) VNR goats. (B) Specific IFN-γ responses at week 28 of the experiment as determined by ELISA after stimulation of whole blood with PPD-B, ESAT-6/CFP-10, or Rv3615c. Results are represented in relation to vaccine cross-protective outcome and are expressed as mean ΔOD450 ± 95% confidence interval. Significances determined by non-parametric Kruskal-Wallis test: *
To further analyze the reductions in post-mortem parameters associated with vaccination, a cross-sectional study was performed on the basis of the criteria described in a previous TB vaccine efficacy trial
Once the subgroup division was applied as described in the previous paragraph, the assumed pathological and bacteriological differences between the new subgroups were checked. The total volume of visible lesions and the bacterial load were significantly lower in VR compared with VNR and unvaccinated control (UC) animals (
The release of IFN-γ specific to E/C, Rv3615c, and PPD-B at week 28 (performed just before sacrifice of the animals) was also compared between the VR, VNR, and UC groups (Figure
Discussion and conclusions
The present experimental trial in goats was designed with the aim to determine the effects of
All experimental goats became infected irrespective of vaccination status. However, a reduction in pulmonary pathology was observed in some vaccinated individuals compared with the unvaccinated group. Another remarkable finding was that all vaccinated goats showed only TB lesions at the site of infection (i.e., lungs and associated LN) in contrast to the increased dissemination frequency in non-vaccinated animals. This finding is analogous to the results of subcutaneous vaccination against PTB with the
In this sense, the present results indicate a certain degree of containment of dissemination of the infection from the primary complex, even when a significant reduction in the bacterial load in pulmonary drainage LN has not been observed. Nevertheless, this containment may not be sufficient to effectively prevent excretion of mycobacteria and horizontal transmission within a herd.
The role of the immunological status of vaccinated animals, especially the T-cell response, can be critical in terms of control or spread of the infection from the primary respiratory focus. Similarly, Hope
After conducting cross-sectional analysis, we found positive correlations of the IFN-γ response to E/C with pathology severity, and more weakly with bacterial load. A similar result was obtained with the single ESAT-6-specific IFN-γ response compared with pathology scores in goats
In terms of assessment of TB diagnostic tests, we have adapted the diagnostic tests routinely used in cattle in bovine TB eradication programs. We have also introduced new DIVA reagents to perform the IFN-γ assay. Sensitivities obtained in all tests were higher than those obtained in previous studies in naturally infected goats
The sensitivities of skin tests performed in vaccinated animals (100% and 90% for SIT and CIT, respectively), were higher than those obtained in another study carried out with goat herds naturally co-infected with TB and PTB (71% and 42.7%, respectively)
By contrast, in our trial, only a slightly higher sensitivity was found when applying the SIT in comparison with the CIT. However, the results showed serious compromise of the specificity of the SIT in vaccinated animals prior to challenge (4 of 4 false-positive reactors), which completely disappeared when using the CIT. These results are in accordance with those found in another study performed in a PTB-vaccinated dairy goat herd free of TB
Compared with the SIT, the standard IFN-γ assay seemed to be more robust in terms of specificity. False-positive results were concentrated at the interval between weeks 12 and 14, and then disappeared at week 16 when there was not yet a response to
Encouragingly, the sensitivity obtained for the standard IFN-γ test after
In the last decade, much effort has been focused on the development of novel antigens for bovine TB diagnosis that are more sensitive and specific than the avian and bovine tuberculins. This research has already resulted in the identification of several antigens, such as ESAT-6, CFP-10, and Rv3615c, that reduce cross-reactive immune responses to different mycobacterial infections or vaccinations in cattle
By contrast, serological responses to the
Vaccination against
Experimental animals
The experiment was carried out in 20 Murciano-Granadina female goats between 2 and 3 months of age from a herd free of TB and PTB in the Region of Murcia (southwest Spain). Before the experiment, goats were submitted to standard tests for diagnosis of TB: the CIT and IFN-γ assay (Bovigam™; Prionics AG, Schlieren, Switzerland). Experimental animals were confirmed to be negative for both tests. In addition, all goats had negative results for PTB (Paratub.Serum-S; Institut Pourquier, Montpellier, France).
Vaccination and infection
A group of 10 goats was subcutaneously inoculated with a single dose of 1 ml (2.5 mg/ml) of Silirum® (CZ Veterinaria, Porriño, Pontevedra, Spain), a commercial heat-inactivated and oil-adjuvanted
The field strain of
All experimental procedures were approved by the Animal Welfare Committee of the Universitat Autònoma de Barcelona in agreement with the European Union Laws for protection of experimental animals.
Sampling and clinical observations
Animals were observed twice daily at feeding time. Prior to vaccination and infection and every 2 weeks throughout the experiment, animals were weighed and their rectal temperature was taken and blood samples were collected from the jugular vein into heparinized blood tubes for immunological studies.
Diagnostic tests
Skin tests
Skin tests were carried out in all goats at 26 wpv and 12 wpi. Four goats were also tested at 14 wpv (the remaining goats were not tested to minimize the potential effect of the intradermal tuberculin inoculation on the IFN-γ assay). Tests were performed by inoculating 0.1 ml (2500 IU) of bovine (PPD-B) and avian (PPD-A) tuberculins (Porriño, Pontevedra, Spain) on the left and the right side of the neck, respectively. The skin-fold thickness was recorded just before inoculation and after 72 h. Results were interpreted either by considering only the increase in thickness for PPD-B (as in a single intradermal test, SIT) or for both PPD-B and PPD-A (as in a comparative intradermal test, CIT). The results were read with the strict interpretation used in bovine TB eradication programs. Goats were considered positive to SIT if the increase in skin-fold thickness at the PPD-B site was ≥2 mm. For the CIT interpretation, goats were considered positive if the increase in skin-fold thickness at the PPD-B site was ≥2 mm and higher than the increase at the PPD-A site.
IFN-γ assay
Whole-blood cultures were performed every 2 weeks in 96-well cell culture plates. Heparinized blood (1 ml per well) was incubated for 24 h at 37°C and 5% CO2 with either PPD-B or PPD-A at a final concentration of 10 μg/ml. Phytohemagglutinin (PHA) (Sigma-Aldrich, Steinheim, Germany) was used as positive control at 10 μg/ml; PBS was used as a negative control. In addition, at 0, 2, 6, 10, and 14 wpi, the peptide cocktail ESAT-6/CFP-10 (E/C) and Rv3615c (Animal Health and Veterinary Laboratories Agency, Weybridge, UK) were used at a final concentration of 5 μg/ml. Plasma supernatants were collected after centrifugation and transferred to a 96-well plate. The IFN-γ enzyme-linked immunosorbent assay (ELISA) was performed according to the manufacturer's instructions (Bovigam™). Optical density was measured at 450 nm (OD450) using a microplate reader (PowerWave XS; BioTek, Winooski, VT). Results were expressed as ΔOD450 (OD450 of antigen-stimulated sample minus OD450 of non-stimulated sample). A result was positive when ΔOD450 > 0.05, whereas in the case of the standard test (using PPD-A and PPD-B as stimuli), a result was positive when ΔOD450 of PPD-B > 0.05 and OD450 of PPD-B > OD450 of PPD-A.
Map IgG ELISA
Plasma samples were analyzed in duplicate for antibodies to
Post-mortem examination
All goats were euthanized at 14 wpi (28 wpv) with an overdose of sodium pentobarbital administered intravenously. They were immediately necropsied to assess the presence and volume of tuberculous lesions in the lungs and pulmonary LN.
Ten percent-buffered formalin was poured into the trachea, which then was tied, and the lungs were subsequently immersed in a container with formalin. After fixation, the lungs were sliced at 4- to 5-mm intervals, and each slice was photographed. Gross lesions were analyzed using image analyzer software (ImageJ 1.43u; National Institutes of Health, USA). The volume of gross lesions in each slice was calculated by multiplying the affected area and slice thickness. The total volume of gross lesions was calculated by adding the partial volumes of gross lesions obtained for each slice.
Lymph nodes
The number of gross lesions and their diameter were recorded for each LN at the time of necropsy. Data were recorded by the same pathologist to ensure measurement consistency. LN pathology scoring was calculated by the approximated total volume of granulomas per sample, calculated using the sphere formula (4/3 ×
Bacterial count
Cranial and caudal mediastinal, tracheobronchial, and retropharyngeal LN were individually weighed and homogenized with 10 ml of sterile distilled water using a tissue homogenizer (Masticator; IUL Instruments, Barcelona, Catalonia, Spain). Homogenates were decontaminated with a final concentration of 0.35% w/v hexadecylpyridinium chloride
Statistical analysis
Student's unpaired two-sample
Competing interests
The co-author Eugenia Puentes belongs to CZ Veterinaria S.A., which produces Silirum® as a prophylactic vaccine against PTB. This does not alter the author's adherence to the journal's policy on sharing data. The rest of the authors declare that they have no competing interests.
Authors' contributions
BPV, RJ, and MD conceived and designed the experiments, analyzed the data, and drafted the manuscript. Regarding conduction of experiments, MD, SL-S, and MN performed the necropsy and evaluated the pathological records, and BPV and MM performed the immunological and bacteriological assays. JG, HMV, BVR, and EP contributed substantively to the scientific discussion of the results. All authors have read and approved the final manuscript.
This study was founded by European Union Project TB-STEP (FP7-KBBE-2007-1-3-04, no. 212414).
We would like to thank Félix García, Ana Andaluz, and Xavier Moll from the Department of Animal Medicine and Surgery of the Universitat Autònoma de Barcelona for their assistance in performing the experimental infection.
We also thank the staff members of the Level 3 Biocontainment Unit of CReSA for their technical and clinical assistance.
We are grateful to the Animal Health and Veterinary Laboratories Agency for supplying the mycobacterial peptide cocktails used as DIVA reagents.
Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle
Spoligotype diversity of Mycobacterium bovis and Mycobacterium caprae animal isolates
Mycobacterium caprae Infection in Livestock and Wildlife, Spain
Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants
Mycobacterium bovis subsp. caprae caused one-third of human M. bovis-associated tuberculosis cases reported in Germany between 1999 and 2001
Mycobacterium caprae in cattle and humans in Croatia
Characterization of mycobacterium caprae isolates from Europe by mycobacterial interspersed repetitive unit genotyping
Zoonotic potential of Mycobacterium avium ssp. paratuberculosis: the current position
Association between Mycobacterium avium subsp. paratuberculosis DNA in blood and cellular and humoral immune response in inflammatory bowel disease patients and controls
Efficacy of commercial and field-strain Mycobacterium paratuberculosis vaccinations with recombinant IL-12 in a bovine experimental infection model
Efficacy of a killed vaccine for the control of paratuberculosis in Australian sheep flocks
Comparative efficacy of an indigenous 'inactivated vaccine' using highly pathogenic field strain of Mycobacterium avium subspecies paratuberculosis 'Bison type' with a commercial vaccine for the control of Capri-paratuberculosis in India
An economic and epidemiologic simulation of different control strategies for ovine paratuberculosis
Significant reduction in bacterial shedding and improvement in milk production in dairy farms after the use of a new inactivated paratuberculosis vaccine in a field trial
Diagnosis of Mycobacterium bovis infection in calves sensitized by mycobacteria of the avium/intracellulare group
Exposure to Mycobacterium avium primes the immune system of calves for vaccination with Mycobacterium bovis BCG
Exposure to Mycobacterium avium induces low-level protection from Mycobacterium bovis infection but compromises diagnosis of disease in cattle
Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay
Evaluation of the long-term immune response in cattle after vaccination against paratuberculosis in two Dutch dairy herds
Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection
Effect of an inactivated paratuberculosis vaccine on the intradermal testing of goats for tuberculosis
Evaluation of specificity of tuberculosis diagnostic assays in caprine flocks under different epidemiological situations
Evaluation of cellular and serological diagnostic tests for the detection of Mycobacterium bovis-infected goats
Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Evaluation of the gamma-interferon assay for eradication of tuberculosis in a goat herd
Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in Cattle
Screening of highly expressed Mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex
Viral booster vaccines improve Mycobacterium bovis BCG-Induced protection against bovine tuberculosis
Experimental infection with Mycobacterium caprae in goats and evaluation of immunological status in tuberculosis and paratuberculosis co-infected animals
Effectiveness and safety of a treatment regimen based on isoniazid plus vaccination with Mycobacterium tuberculosis cells? Fragments: field-study with naturally Mycobacterium caprae-infected Goats
Outbreak of tuberculosis caused by Mycobacterium bovis in golden Guernsey goats in Great Britain
Tuberculosis in goats
Correlation of ESAT-6-specific gamma interferon production with pathology in cattle following Mycobacterium bovis BCG vaccination against experimental bovine tuberculosis
Association of tuberculin-boosted antibody responses with pathology and cell-mediated immunity in cattle vaccinated with Mycobacterium bovis BCG and infected with M-bovis
The effect of Johne's vaccination on tuberculin testing in farmed red deer (Cervus elaphus)
Effect of vaccination and natural infection with Mycobacterium avium subsp. paratuberculosis on specificity of diagnostic tests for bovine tuberculosis in farmed red deer (Cervus elaphus)
Evaluation of the cell-mediated immune response to reduced doses of Mycobacterium avium ssp. paratuberculosis vaccine in cattle
Field evaluation of a novel differential diagnostic reagent for detection of Mycobacterium bovis in cattle
Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks
Goats primed with BCG and boosted with a recombinant adenovirus expressing Ag85A show enhanced protection agaist tuberculosis
Evaluation of a Pourquier ELISA kit in relation to agar gel immunodiffusion (AGID) test for assessment of the humoral immune response in sheep and goats with and without Mycobacterium paratuberculosis infection
An evaluation of 1-hexadecylpyridinium chloride as a decontaminant in the primary isolation of Mycobacterium bovis from bovine lesions


text:
VideoHive Intro Projects 164715
After Effects Project Files \ Logo Stings \ Abstract
Intro Projects 164715 by BrokenDancer @ VideoHive
After Effects Project Files \ Logo Stings \ Abstract
After Effects Version: CS4, CS5 File Size: 275.5mb Requires Plugins: No Resolution: Resizable
Download this after effects project file and enjoy this cool effect.
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Have fun!
Any question ? just send me a message through my profile. If you don't have the right skills, I can help you out, no prob.
Download Link

subset: commoncrawl


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
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text:
Global Semiconductor Sales Remain Flat in August
by Dan Rosso
WASHINGTON, D.C.—Oct. 2, 2012—The Semiconductor Industry Association (SIA), representing U.S. leadership in semiconductor manufacturing and design, today announced that worldwide sales of semiconductors reached $24.30 billion for the month of August 2012, a slight increase of 0.1 percent from the previous month when sales were $24.27 billion and a 3.2 percent decrease from the August 2011 total of $25.1 billion. Year-to-date sales reached $189.46 billion in August 2012, down 4.6 percent compared to the same time last year.
"Global semiconductor sales have held steady in recent months despite strong macroeconomic headwinds, but these challenges have hampered growth," said Brian Toohey, president & CEO, Semiconductor Industry Association. "With Election Day fast approaching, candidates should have a vigorous discussion about enacting government policies to reduce business uncertainty, accelerate the economic recovery and keep America at the forefront of innovation."
Year-over-year and year-to-date sales were lower across all regions, with small decreases in Japan and Asia Pacific and steeper drops in Europe and the Americas. Sales increased on a sequential monthly basis in the Americas (1.0 percent) for the first time since April 2012. Japan (1.0 percent) also increased month-over-month, while Europe (-1.9 percent) fell slightly and Asia Pacific remained flat. All monthly sales numbers represent a three-month moving average.
About the SIA
The Semiconductor Industry Association, SIA, is the voice of the U.S. semiconductor industry, one of America's top export industries and a bellwether measurement of the U.S. economy. Semiconductor innovations form the foundation for America's $1.1 trillion dollar technology industry affecting a U.S. workforce of nearly 6 million. Founded in 1977 by five microelectronics pioneers, SIA unites over 60 companies that account for 80 percent of the semiconductor production of this country. Through this coalition SIA seeks to strengthen U.S. leadership of semiconductor design and manufacturing by working with Congress, the Administration and other key industry groups. The SIA works to encourage policies and regulations that fuel innovation, propel business and drive international competition in order to maintain a thriving semiconductor industry in the United States. Learn more at www.sia-online.org
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- Global Semiconductor Sales Decrease Slightly in August
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Articles tagged duncan sheik
7 Apr 2016 // 2:30 AM
- //Features
After winning a Tony for his work on Spring Awakening, pop craftsman Sheik now tackles bringing American Psycho to the stage.READ more
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Duncan Sheik returns with a vibrant new track from his eighth album.READ more
21 Jan 2010 // 11:00 AM
SAN DIEGO — Duncan Sheik is a skeptic of the supernatural — "I completely don't believe in ghosts," the singer-songwriter says. Yet if his new musical "READ more
13 Mar 2009 // 7:00 AM
With Broadway's "Spring Awakening," Duncan Sheik transformed himself from lite-rock crooner ("Barely Breathing") to Tony-winning composer. He's not done yet _ Sheik's newREAD more
26 Jan 2009 // 9:00 PM
A concept album from a pop survivor. Great concept, but the "album" part falls short.READ more
11 Dec 2008 // 9:00 PM
On this particular night Duncan Sheik confirmed his seat at the musical theatre head table.READ more
11 Jun 2007 // 3:15 AM
- //News
And the big Tony Award winner on Broadway is - the 19th century! Two stage works set in the 1800s were named the season'sREAD more
7 Jun 2007 // 11:00 PM
Songwriter Duncan Sheik talks with PopMatters about the musical Spring Awakening, nominated for 11 Tony Awards, and writing music that's relevant to a larger cultural argument.READ more
26 Feb 2001 // 3:00 PM
Duncan Sheik was introduced to the world with his hit "Barely Breathing" in 1996 and in a Sarah MacLachlan world, people responded positively to his sensitiveREAD more
Jillette Johnson 'All I Ever See in You Is Me' (album stream) (premiere) // Music
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'Doctor Who': Casting a Woman as the Doctor Offers Fresh Perspectives and a New Kind of Role Model
// Channel Surfing
"The BBC's announcement of Jodie Whittaker as the first female Doctor has sections of fandom up in arms. Why all the fuss?READ the article


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General UFC/MMA/boxing discussion
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Kriss E
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Originally Posted by
I'm curious to the answer of your last question as well. I guess when people say GSP has the advantage on the ground they're assuming he'll be in top control, which is a pretty fair assumption, however, GSP on his back isn't impressive at all, he's not in Silva's league from his back.
We seen in the 2nd fight that with Sonnen that Silva has pretty good take down defense as well. The problem lies that he is usually comfortable off his back. He learned from the 1st fight that being on your back against an outstanding wrestler like Sonnen was not in his best interest. Sonnen got him down in the first, but after that he was stuffed. To me, Sonnen is a better wrestler than GSP.
Th only other guy I'd put in Silva's league is Fedor, but that was the Fedor of a few years, not the current one. His reign has ended. So right now, It's all Silva.
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Android: A Programmer's Guide shows you, step-by-step, how to download and set up all of the necessary tools, build and tune dynamic Android programs, and debug your results. Discover how to provide web and chat functions, interact with the phone dialer and GPS devices, and access the latest Google services. You'll also learn how to create custom Content Providers and database-enable your applications using SQLite.
Table of Contents
Ch 1. What is Android
Ch 2. Downloading and setting up Eclipse
Ch 3. Downloading the Google Android SDK and Eclipse Plugin
Ch 4. Exploring the Google Android SDK
Ch 5. Application: Hello World!
Ch 6. Using the Google Android Emulator
Ch 7. Reacting to phone events
Ch 8. Calling the GPS
Ch 9. Application: Note Block
Ch 10. Buttons, Lists, and other Objects
Ch 11. Application: Caller Info
Ch 12. Application: Find Me
Ch 13. Interacting with other Google Services
Ch 14. Troubleshooting and Errors
You can download this book from any of the following links. If any link is dead please feel free to leave a comment.
Download here
Copyright Disclaimer
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.


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Do I Have To Freeze Them?
Baking By cindylou2 Updated 11 Apr 2011 , 3:13am by Cupcations
I just made 5 dozen cupcakes for a function tomorrow evening but I'm tired now and wont be able to make my icing and frost them until tomorrow. I have them in soem sealed containers but would need to make room in my fridge if I have to keep them cool. Would they be ok to remain on the counter overnight so I can frost them tomorrow?
I would.. I mean its for less than 24 hours right?... plus if your worried & you don't have enough room in the fridge refrigerate each dozen(or two) for 5hrs & then take them out & put the others
IMO they should be ok & fresh untill the event
Quote by @%username% on %date%


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In the meantime I thought I'd offer an update on what's new and interesting around these parts.
- Georgia has started to climb stairs. She does this quickly and with great pride. It only terrifies me slightly.
- On Saturday I got to hit up one of my favourite thrift spots - St Vincent de Paul in Belleville. Who am I kidding? It's my favourite thrift spot. I scored some amazing textiles. Yards and yards of tacky cat prints and pure grey wool.
- Found and purchased these lovelies for the little miss.
- Been working hard on developing a few new products for the shop. Until then I still have lots of half price chalkboard and simple totes in stock!
- I've also been planning fun blog events for the next few months. I know I've been remiss in my blogging duties (blame it on the product development), so I want to make it up to you all with some really rad stuff very soon.
See you soon lovelies!


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SJHC Saturday Childbirth Edcucation Class
Mercy Health
Saturday, April 5, 2014 at 9:00 AM - Saturday, December 6, 2014 at 3:30 PM (EDT)
Event Details
This 6 1/2 hour class includes a tour of Labor and Delivery and is offered one Saturday each month. Topics includes: anatomy and physiology of the labor process, hospital interventions, Cesarean sections, post-partum and Lamaze breathing and relaxation techniques. A Partner or Coach is welcome to attend. Registration is required and requested at least one month prior to class date. St. Joseph offers a variety of classes to help prepare you for life with baby. Classes offered are Childbirth, Successful Breastfeeding, Infant Resuscitation Basic Skills and On-line Childbirth Education Class.
|Saturday, April 5 2014
|Saturday, May 3 2014
|Saturday, June 7 2014
|Saturday, July 5 2014
|Saturday, August 2 2014
|Saturday, September 6 2014
|Saturday, October 4 2014
|Saturday, November 1 2014
|Saturday, December 6 2014
When & Where
St. Joseph Health Center
667 Eastland Avenue Southeast
Saturday, April 5, 2014 at 9:00 AM - Saturday, December 6, 2014 at 3:30 PM (EDT)
Add to my calendar


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Beautiful 4.3 acre country building site w/incredible views & mature trees . Perfect spot for house & pole shed/horse barn setup. Zoned RH-1 so many uses possible and driveway is already in. Property is only 2 mins. to Hwy 19 or Hwy T so quick access in many directions. You truly have to stand on this property to appreciate it. This property is 2 parcels being sold together w/1 building site.
Features Lots and Acreage
|Total Acres: 4.23
|Zoning: RH-1
|Present Zoning: Agricultural, Other, Single family
|Lot Size: 4.23
|Lot Description: Adjacent to park/public land, Rural - not in subdivisn
|Taxes: $255
|Elementary School: Marshall
|Middle School: Marshall
|High School: Marshall
|School District: Marshall
|Body of Water:
Estimate your monthly mortgage payment
How much can I borrow?
Speak with a mortgage consultant: 866-531-0904


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A Family's Guide to Tackling Eating Disorders
by Karen Asp
Women with eating disorders aren't the only ones who suffer. The struggle becomes a family affair, one that's even more difficult when the woman is on her own. While there are things families shouldn't do and say, there are just as many things they can do to help not only their loved one, but also themselves.
The Long Road to Recovery
Learning that someone you love is suffering from an eating disorder is never easy. The initial diagnosis may come as a shock, but for some people and their loved ones what comes after the diagnosis can be even harder. Successful treatment of eating disorders is a long and often difficult process. Progress is irregular and setbacks may be many.
"Progress in an eating disorder is measured in years," says Carolyn Costin, MA, MEd, MFCC, director of the Monte Nido Treatment Center in Malibu, California. "Recovery will take a long time, and you have to prepare yourself for that."
It is important for affected women and their families not give up hope. Bulimics and anorexics who want to get better can recover. "Recovery is attainable, but it's a matter of being patient and allowing that recovery process to happen," says Julie Clark-Sly, PhD, co-clinical director at the Center for Change in Orem, Utah.
Understandably, though, there will be times when your patience is tested. When you watch your loved one engaging in destructive behaviors. When everything you say or do triggers a defensive response. So what can you do?
Educate Yourself
Perhaps the first step in helping your loved one recover is educating yourself about eating disorders. People often assume that eating disorders are about food and weight, but that's not true. "Eating disorders are about underlying issues," says Julie DeLettre Holland, MS, an Atlanta-based certified eating disorder specialist and director of outpatient professional development with the Renfrew Center.
To deal with those issues, hopefully you've encouraged your loved one to seek professional counseling. If her health is in jeopardy, you may have forced her into counseling. Consider asking her therapist to set up educational sessions for your family. The therapist may also suggest family counseling. In almost all cases a medical consultant, either a generalist physician or a psychiatrist, will also need to be involved.
As you read books or attend educational meetings or family support groups, don't let the eating disorder consume you. "You have to take a break from the problem, especially if your loved one lives at home," Clark-Sly says.
Dismiss Guilt
Guilt may eventually catch up with you, as it does for many families. How could you have let her suffer with this eating disorder for so long? How could you have prevented it? What did you do to contribute to it?
Experts say you shouldn't blame yourself. "There's no single cause for an eating disorder," Clark-Sly says. "You have not created the disorder." Every family makes mistakes, she adds. And certainly, if you're doing things that are contributing to the problem, such as being abusive, then you need to correct those things.
But you can't blame yourself for what you have—or haven't—done. "If you're focusing on that," Holland says, "then you won't be able to move forward and learn how to help."
Costin encourages families to stay solution-oriented. "You can have all the insight about why someone needs to control her food intake," she says, "but that won't change anything."
The Do's and Dont's of Helping Her Recover
The best thing that you can do is provide support. "She needs the family to be a steady, stable force of love and support," Clark-Sly says.
Yet there are actions you can take to make things easier for you and your loved one:
1. Don't let your relationship with her have a one-sided focus on her. You're an important person too. When you're talking about her day, for instance, share information about your day, Holland says.
2. Keep the focus off of food. Whether you're at a family reunion or the dinner table, "focusing on food creates disaster," Clark-Sly says. Talk about the day's events, go for a walk, or play board games.
3. Legalize all foods. "It's not healthy to cater to the belief that foods are good or bad," Holland says. Costin, a recovered anorexic, also suggests offering to serve something she'll eat if you're hosting the event. At holiday meals, her mother used to serve vegetables and salad so that it was less conspicuous that she wasn't eating gravy and stuffing.
4. Be a good role model. Don't criticize your own eating habits or weight. Don't encourage anyone to pursue slenderness, and don't admire weight loss or excessive exercise. Don't start dieting or keep a scale around the house. "You have no idea how much this affects her," Costin says.
5. Don't change the family's eating patterns. Her eating disorder should not control how other members of the family eat and live, especially if any change comes from you deciding what will help her. If she feels that specific family changes would support her recovery, then as family members you can decide if and how those changes might be made.
6. Ask her how she wants you to support her, Clark-Sly says. You don't have mind-reading abilities. Talking about how you can help is the healthiest way of dealing with things.
7. Don't try to take a certain behavior away from her. "You can't take a behavior away until there's a healthier one to put in its place," Holland says.
8. Avoid responding to "I feel fat" comments. Hopefully she is in cognitive behavioral therapy, and both you and she will have a specific plan to deal with these kinds of "intrusive thoughts". If there is no plan, then an honest way to address an "I feel fat" comment might be as follows: "I can understand you might feel that way, and that feeling fat might be very uncomfortable for you. However, those feelings are part of the illness of an eating disorder; they may feel real to you but they are not real, and to recover you will have to give them up. It would help me if you could tell me about a real feeling you have, for example it would help if you could say, "I feel sad" or "I feel angry." We could talk about feelings of sadness, or fear, or anger, especially if you were able to say "I feel angry when you …"
9. Don't ignore destructive behaviors. Doing this empowers the eating disorder, Holland says. When you see her engaging in odd destructive behaviors like binging, purging, or not eating at all, show that you care. Ask if anything is going on or if she wants to talk.
10. Make her accountable for her actions. If she eats all your food or vomits in the toilet, ask that she replace the food and clean up her mess, Costin says.
11. Don't be afraid to approach her therapist with your concerns. Therapists won't reveal confidential information but they can discuss your concerns, Costin says.
12. Remember that there's no right or wrong reaction. "If you're always worried about how to relate, then you'll be fake and rigid," Clark-Sly says. "Be yourself and follow your heart."
American Anorexia/Bulimia Association
Laureate Psychiatric Clinic and Hospital
National Eating Disorders Association
The Renfrew Center
Sunday, March 12, 2006
A Family's Guide to Tackling Eating Disorders
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She Males With Big OnesFavorites [+]
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We have an 8 yr old and 2 yr old lab, they both get this supplement daily, their coats are beautiful - the older guy has some immune deficiencies and I firmly believe these help him. We used to purchase the larger size bottle, but it was recently not available - hope it comes back in to stock soon!

Our GSD has been given these for about a year. She has an autoimmune issue and it helps with the reduction of inflammation.
I have been using this for a couple of years with now my 14 year old Lab and 14 year old Hound dog. It helps both with making coat look nice and ease of movement.
We have a American Bulldog mix who has allergies and skin issues. The results are stunning within one week. Hair growth returned that was shinny and glossy and he was not scratching as much. The price is fantastic and the quality is excellent. Any dog who has skin issues should try this!!!!!
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This email was created by [MemberName] <[MemberEmail]> who thought you would be interested in the following Article:
"Integrating Performance Profiling into the Build Process" A look at how performance profiling can be integrated into the build process to form part of the automated test suite.
CodeProject also contains hundreds of articles, free source code samples and applications to help in your day to day programming. Why not give it a try!


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Tag: urbex
The Asylumwritten by dazb on 2010-02-10 #places
Hiding deep within the English countryside lies this spooky derelict asylum...19 36 Share Tweet
Chernobyl/Prypiat: A Silent Citywritten by pinkpix on 2011-11-06 #places
September 2011, Marije (s0y) and I finally took the trip we wanted to do for so long, visit Ukraine, Kiev and in particular, the 30km exclusion zone around Chernobyl, with the ghost town of Prypiat. When I saw the bumper carts, I almost cried. Afterwards, we were exhausted; there was so much to take in. And bringing too many cameras is never a good idea. ;)11 18 Share Tweet
Utrecht Urbex: The Cereol Factorywritten by marije on 2011-06-02 #places
Looking for an easily accessible urban exploration location? Look no further than Utrecht's Cereol Factory.6 10 Share Tweet
Analogue Urban Exploring in Dadiparkwritten by pinkpix on 2011-08-21 #places
Together with a small group of friends, I went to the abandoned amusement park 'Dadipark', which closed almost 10 years ago after a series of accidents in which a 9-year-old boy lost his arm. Although more and more people find their way to this park as it is an open secret, it is still quite intact. It was freaky and fascinating. And definitely worth a visit.6 9 Share Tweet
Derelict Military Barrackswritten by dazb on 2011-03-20 #places
On the outskirts of Bath, one of the UK's most popular tourist cities lies this crumbling derelict military barracks. It opened in the 1940s and closed over 50 years later in 1993. A few years ago there was "news" that the site would be converted into retirement homes - nothing seems to have happened with that plan though.1 5 Share Tweet
Henryton State Hospitalwritten by edenhovenga on 2013-02-06 #places
Henryton State Hospital, known as Henryton Tuberculosis Sanitarium, is a now abandoned hospital complex located in Marriottsville, Maryland built in 1922.3 3 Share Tweet
Nine Levels Copper Refinery Taiwanwritten by dragontw on 2012-06-27 #places
Doing some Urbex photography has been one of my photography goals this year, but it took awhile to find a good first location. With a bit of research I found out about an old Copper Refinery that dates back to the Japanese Occupation in Taiwan. It closed down in the early 70's and was left abandoned for nature to take over. It's located on the north coast of Taiwan and it would be a full day trip to reach the location.2 3 Share Tweet
Tales from Nichitsu: Haikyo in Film by Riccardo Parentiwritten by plasticpopsicle on 2013-10-16
Last week, we introduced you to Riccardo Parenti's beautiful film photographs around Nichitsu, a popular ghost town around three hours away from Tokyo. We got in touch with Parenti soon after and asked him to tell us more about his ongoing project and experiences in the famous haikyo ("abandoned place").4 Share Tweet
Three Abandoned Engine Sheds in Stralsund, Germanywritten by inrod on 2013-10-10 #places
While preparing for this year's vacation, I stumbled across three abandoned engine sheds close to the train station in Stralsund, Germany. I decided to explore this spot.1 2 Share Tweet
Exploring the Jacob Tome School For Boyswritten by edenhovenga on 2013-04-19 #places
The Tome School for Boys, located in Port Deposit, Maryland, was founded by Jacob Tome as a preparatory school for boys; and it first opened in 1894.1 1 Share Tweet


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She said the petition bore the names of 250 residents throughout town.
Residents have been campaigning for months to prevent Alexander Avenue from becoming a parking area for new businesses on nearby Main Street.
The petition-signers want Alexander blocked 200 feet in from Main Street, where the commercial district meets the residential zone. They also want the signs changed from "One-hour parking 8 a.m. to 7 p.m." to "One-hour parking 8 a.m. to 10 p.m., no loading or unloading any time."
The petition said in part, "Alexander Avenue has been tremendously impacted by increased traffic, loading and unloading of Jaguar dealership cars, road-testing of the dealership's cars, and numerous other cars parked by employees of businesses in the vicinity, including the parking of those vehicles on the sidewalks.' '
Said DeRosa, "These preventive measures will ensure our safety and quality of life. Increased traffic, because of development, added to the already congested area, will only lead to more problems for pedestrians, school children, motorists and, ultimately, the residents of Alexander.
"Main Street at Alexander has changed dramatically," DeRosa noted. "Anyone who is a long-term resident recognizes this immediately. Business activities flourish, which is good, but at what cost to children, motorists and, ultimately, the residents? Now, we are faced with the development of property across from Alexander Avenue, on the shopping center side of Main Street. There are other business properties as well on that side of Main Street that are begging for upgrade, and it will happen," she said.
Council President Mary-Anna Holden said she and Sgt. John Sabanosh, who heads the police department's traffic safety unit, walked and took measurements at the proposed site of the cul-de-sac. They came up with a concept for a crescent-shaped barrier that would allow cars to be driven in and out of the rear of the Jaguar agency's property, she said. Because Alexander Avenue is designated a feeder/collector street, the change would require the approval of the state Department of Transportation, she added.
Restaurant Proposal
Last winter, Alexander Avenue residents lobbied the Planning Board to deny Parsippany developer Joseph Lobozzo's application to convert the former Madison Printing Company building on Main Street into retail shops and offices. The plan included razing part of the building to provide 18 on-site parking spaces in addition to the one that existed, and using another 10 parking spaces along Main Street.
The planners rejected Lobozzo's proposal on Feb. 6. His attorney, Alan Rich, app eared before the board informally on April 24 with a revised plan one that does not require waivers, because Lobozzo no longer proposes to change the "footprint" of the building.
Under the new plan, there would be a 50-seat restaurant, a dry cleaning drop-off /pick-up shop, and offices upstairs. There would be only one parking space on the grounds, as there was when the building still housed a printing business, last year.
The latest plan was scheduled to be heard by the Planning Board on Tuesday, June 5, but a crowded agenda prompted the board to reschedule the hearing to Thursday, July 5.
The upshot is that Alexander Avenue residents now face the possibility of having more people park on their street than they did before.


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The World Cafe Design Principles
The following set of seven integrated design principles has been developed over the years as a means of intentionally harnessing the power of conversation for business and social value. These simple principles, when used in combination, provide useful guidance for anyone seeking creative ways to foster authentic dialogue in which the goal is thinking together and creating actionable knowledge.
1. Clarify the Context
Clarify the purpose and broad parameters within which the dialogue will unfold.
2. Create Hospitable Space
Ensure the welcoming environment and psychological safety that nurtures personal safety and mutual respect
3. Explore Questions That Matter
Focus collective attention on powerful questions that attract collaborative engagement.
4. Encourage Each Person's Contribution
Enliven the relationship between the "me" and the "we" by inviting full participation and mutual interaction.
5. Cross-pollinate and Connect Diverse Perspectives
Intentionally increase the diversity and density of connections between perspectives while retaining a common focus on core questions.
6. Listen Together for Patterns, Insights, and Deeper Questions
Focus shared attention in ways that nurture coherence of thought without losing individual contributions.
7. Harvest and Share Collective Discoveries
Make collective knowledge and insight visible and actionable.
In addition, these simple rules of etiquette will help you get the most from your World Café conversation:
1. Focus on What Matters
2. Contribute Your Thinking
3. Speak Your Mind and Heart
4. Listen to Understand
5. Link and Connect Ideas
6. Listen Together for Insights and Deeper Questions.
(Playing, Doodling, Drawing are all encouraged!)
7. Have Fun!
In world adapters of The World Cafe ideas are Rockridge Institute and The Grove.
If you are interested in having a World Café conversation as part of your inworld event, contact World Café Community Tech Steward Pipi Tinlegs.


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GreenHouse Coffee: The off-Broadway coffee shop is now pouring
Andrew Wise
Tucked into the matrix of warehouses and industrial buildings near I-25, Santa Fe and Evans is GreenHouse Coffee, a large green bungalow plopped across the street from the Evans light-rail station at 2190 South Delaware Street. Open from 6:30 a.m. to 1 p.m. Monday through Friday, this cozy spot arms bleary-eyed commuters with a morning buzz, and also provides the neighborhood with a place to enjoy a quiet cup of Pablo's coffee or a Trompeau Bakery pastry away from the traffic of Broadway.
Andrew Wise
Owners Mark Carl and Maria Martin appreciate the niche they found in this part of town. "We're just trying to focus on being the small, local coffee shop with a dog-friendly atmosphere," says Martin.
They plan on paying special attention to the needs of Denver's dog owners. Besides the usual jar of dog treats, the coffeehouse has a massive yard that will become a haven for neighborhood people and pets come spring -- even if the yard and patio furniture now sit under snow.
GreenHouse opened quietly on November 14. "We planned for a soft opening," Martin explains. "We'll be ready for a big publicity push at the beginning of the year."
In the meantime, the coffee's on. Find more information on the GreenHouse website.
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non-consensual post-dada constructivist cerebral warts
2012 Feb by Mike Davis
I was working late at the office last night when I got a message from my friend DJ Ayres. I've worked on a considerable number of graphic design projects for Ayres over the last few years, more than I can count on both hands, and he keeps up with my other design work just as I do with his music projects. He sent me a link to the Facebook page of a young DJ named Nate Starr living in Springfield MO. This DJ's profile picture was an exact replica of the "Jump On It" EP cover I designed for Minneapolis DJ and producer Last Word.
My original design for DJ Last Word
The Nate Starr graphic in question
Nate essentially saved the EP artwork to his computer, opened up the file in Photoshop, erased Last Word's name and album title, replaced them with his own, and hit SAVE.
On one hand, I feel flattered. This Nate guy was looking around the internet, stumbled across an image I spent a lot of time and energy creating, and then said "Yes. This is it. This is the image that says everything about DJ Nate Starr." That someone was so drawn to something I made so much that they felt the need to take it and make it their own really says a lot to me.
EXTRA LARGE flyer designed by me.
BANG BANG PARTY flyer by Samantha Cake Robles
On the other hand, this is just another example in the huge landfill of examples of modern-day kids thinking they can get away with jacking other artists' work and calling it their own. Actually, scrap that… I wish I could say it's just uneducated, inexperienced kids making this mistake, but this act of unabashed thievery happens all the time at the desks of highly-paid designers working for big companies. Why do some people think it's OK to steal others' artwork? Why are some designers satisfied with signing their names to someone else's work and calling themselves designers? Even with a tight-knit online community of designers keeping a careful collection of eyes peeled for thieves, ready to blast the floodlights and sound the alarm at a moment's notice, there are still countless Photoshop hacks who think they can rob from the talented and get away clean.
original artwork by Minneapolis artist Brock Davis.
The Books concert poster by Paul Gardner
We live in a disposable culture right now. Everything that happens is uploaded, viewed, liked, favorited, shared, downloaded, remixed, parodied, autotuned, reuploaded, destroyed, ridiculed, and forgotten about all in a matter of hours. Images, videos, jokes, and memes come and go at a blinding speed, hardly allowing for any time to enjoy or reflect before it's time for the next one. People are devouring images without stopping to wonder where they come from, who made them, or what they mean. Because information is so readily available and easily acquired, many Photoshopsters feel a sense of entitlement, as if everything on the internet belongs to them and is theirs to do with as they please. I've heard every excuse from "Well I didn't even get paid for doing it, so it doesn't matter" to "I can take whatever I want and do whatever I want to it!" to "I was looking through some illustrations on my computer and forgot which ones were my own artwork and which ones I just found online as inspiration."
L: original artwork by designer Jon Contino. R: t-shirt not designed by Jon Contino available at Forever 21
When you steal someone's artwork, music, logo, or video, you don't just steal the time the artist spent creating that one piece, you steal the years it took for that artist to hone their craft. You steal all the years they spent studying, sketching, researching, scanning, tweaking, making mistakes, redesigning, negotiating, pitching, polishing, and refining. I've copied tons of work from some of my favorite artists and designers. But copying is never the final step - it's part of a lengthy learning process. In copying their work, I studied what they did, tried to figure out how they created their work and then added that information to a vast cache of ideas and influences, eventually creating my own style and work. If you happen to end up dining on someone else's work, the least you can do is pay compliments to the chef.
So that's that. I don't know if Nate Starr or any of the other people who have been overly-influenced by my work will ever read this, but I certainly hope they do. I hope that the current generation of young artists and designers can leave behind a body of original, inspiring artwork, cause I want to keep seeing new art and images and not just keep seeing the work of my friends and peers regurgitated.
Shortly after this blog post was published, I received an email from Nate Starr apologizing for copying my work. He's also taken down the artwork shown at the beginning of this post.
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JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
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Captcha not workingAsked by khathutshelopetrus on October 14, 2014 at 10:32 PM
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The captcha on your standalone forms are working well here. Are you having issues with the embedded forms? If so, can you please share to us the exact URL where the captcha forms are embedded?
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Lauren unlocked the front door of the house and stepped inside just as the sun began to set past the trees that enclosed the area of her home. Inside, she slid off her shoes and remembered to check both locks on the door before she walked into the living room and tossed her book bag on the couch. She picked up the remote control off the coffee table that sat between her and the TV and began flipping through channels until she found a show on she could withstand hearing for a while. It wasn't really about what she'd want to watch, but more or less something she could have on while being home alone for the remainder of the night; it gave her a sense of closure being out in the country by herself.
Both parents had gone out of town for a couple of days and Lauren was able to convince them that she was old enough to stay here and take care of herself while they were away. Looking about the empty two story building now, though, she sort of regretted the choice. She wasn't necessarily scared in any way, like she used to be as a little girl, but the atmosphere of it all was a bit unsettling; especially with it getting dark.
Looking for something to do, Lauren stumbled into the kitchen. She browsed through the cabinets and refrigerator before grabbing two bags of junk food and a refreshing soda. As she turned to head back into the living room, however, she spotted something sitting on the dining room table. It was a package like one you receive in the mail. Written in black marker on the top was her name; Lauren Smith. No return address, no other details. Lauren placed the food down and looked the box over to see who it was sent for. When she came up empty handed, she pulled out her cell phone from within her pants pocket and dialed.
"Hello?" A women's voice answered on the other end.
"Hey, mom. It's Lauren. I was just calling to see if you guys were expected something in the mail any time soon? Maybe something you wanted me to have?"
A pause, "No…no I don't think so. Your father said he wasn't getting anything today either. Why do you ask?"
"There's a package here with my name on it. I figured it was from you guys."
"Hm, well don't open it until we get home okay? We don't know what's in there exactly."
After a small chat with both parents on how their trip was going, Lauren hung up the phone and went back into the living room with her snacks; plopping down into the couch for the rest of the afternoon. The package remained where she found it. However, after finishing her snacks and finding nothing left on TV to watch, Lauren soon became bored and her curiosity caught up with her.
She walked back into the dining room and stood in front of the mail. Glancing out the window across from her and catching glimpses of trees swaying in the night breeze. Finally, after a moment's hesitation, Lauren began to cut into the packaging using a nail. Once removed, she gazed at nothing more than a simple black box with a latch keeping the lid closed. As with the outer packaging, she examined the box for any name or address but upon finding down, decided to lift up the latch see what lie inside.
A bundle of photographs wrapped together with a rubber band. That was the boxes contents. Interested, Lauren reached in and retrieved the photos, snapping off the rubber band and taking a seat as if what was taken in the photos was too important for her to remain standing. The first one, sitting on the very top of the stack, was of a man. His face was scribbled out in black marker, the same one Lauren figured as the one that her name was written in. The man was tall and wore clothes that seemed a couple of sizes too small for him. He stood in a hunched, slacking position in the middle of the woods.
Lauren wrinkled her brow and continued with the second picture. It was the same man in this one, his face still marked out, yet this time he stood in a field just outside the trees. For some reason, this location seemed familiar to Lauren; making her feel uneasy, yet she pressed on. The third photograph was very unsettling. The man was now looking through a window, yet his face wasn't marked out as his hands cupping around his head blocked his features with shadows.
Next, the man was at the top of a set of stairs, looking down. His face crossed out with a big x, again, with the same marker. Finally, the last of the photographs was of the man standing in the middle of a kitchen, one that Lauren knew all too well. In his hands was a small black box. This time, the man's face was not scratched out and he had a sinister smile etched on him.
Lauren held tightly to the photos in her now shaking hands; her mouth opened slightly in a gasp. From behind, she heard the click of a camera and then something drop beside her feet. She slowly directed her gaze onto a photograph lying on the floor of her back…as she flipped through the photographs.


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Free Education Programme Benefits End Users In fight Against Cybercrime
InfoSecurity Europe
: 24 October, 2011
(Company News)
Recent e-mail ransom case highlights importance of increasing awareness amongst employees of vulnerabilities to cyber-crime
Commenting on a weekend report in the Guardian newspaper about a journalist whose email account was held to ransom, the organisers of the Infosecurity Europe show say that the case highlights the need for better education on the IT security front for members of staff in organisations of all sizes.
According to Claire Sellick, the show's Event Director, the journalist concerned had probably `clicked through' on a link which infected her computer, allowing the hacker remote access to her Gmail account. "This was, of course, only a personal email account. You can image the chaos that would ensue had the hacker gained access to an admin or sub-admin account for a corporate, as several hundred - or thousand - users could have been affected. Our observations amongst the many tens of thousands of visitors to the Infosecurity Europe exhibition each spring, however, suggest that it is the staff within mid-sized companies that need education about the `total security picture' for their IT systems."
The show director went on to say that, where smaller firms are concerned, since they tend to use low-cost and even free self-service accounts - their access to support services when serious information security issues do arise, as happened in this case, tends to be limited. Sellick says that the case also highlights the fact that, whilst the blackmailed journalist writes about political matters for the Guardian, the FT and the Statesman, you clearly cannot expect her to be fully conversant with the latest aspects of IT security. Nor, in fact, she explained, can one expect most people – even inside of IT security - to be totally up-to-speed on the latest threats to their organisation's digital assets. This is where Infosecurity Europe's popular education programme enters the frame.
The Infosecurity Europe event director says that one of the most popular features of the event continues to be the free educational programme - with leading IT security professionals sharing their knowledge and expertise in a wide selection of seminars and panel discussions for attendees.
"One feature of the attendances at the April 2011 event was that many professionals were in an area of management which was not directly involved with IT security on a day-to-day basis, reflecting the fact that IT has become pervasive in business generally." Said Sellek, "but just because IT has become so integral to modern business does not mean that an understanding of IT security goes with that pervasiveness. This is why the next Infosecurity Europe event – which takes place at London's Earls Court on the 24/26 April 2012 – will feature a free education programme as one of the key attractions."
"This isn't to say that education would have prevented this journalist from having her mailbox held to ransom for £500.00. Our observations, however, suggest that education could have prevented the unfortunate situation from happening in the first place." She added.
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Scal For Mac Users, Any Advice?
Decorating By cakesage Updated 17 Dec 2010 , 2:12pm by Dayti
Finally got my Cricut Cake machine to cut nicely. I'm interested in purchasing the SCAL software. I own a Mac. Does anyone have any advice, tips or suggestions. I know that there is a Mac version of the software program but I keep reading something about firmware compatibility.
Would like to know your experiences Mac users.
Thanks in advance!
I have bought the SCAL 2 software to use with my new Cricut Cake. I also run a Mac, but appear to be having problems with the firmware, although the correct version is on the Cricut.
Does anyone else use a Mac with their Cricut Cake? Any pointers at all?!
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- Doctors
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- Dr. Jehangir P. Mistry
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Ratings for Dr. Jehangir P. Mistry
I went in for some antibiotics for a UTI and I got yelled at for engaging in pre-marital sex. I was shocked and in tears and really angry afterwards that I did not just slap him in his office. He also was extremely rude and condescending towards my mom on her office visit. She also left in tears without even getting the help she needed. He should NOT be a doctor.
He thinks he's God. When you go and see this doctor he starts telling you off right away. He doesn't address your issues properly. He's rude, abrasive and abusive. You basically leave there overwhelmed, upset, and in a state of shock. Have discussed his behavior with other patients, and all have had the same horrible experience. Schedules unnecessary tests and follow-ups for the sole purpose of billing you more. When I told a nurse at the local hospital that he was my doctor, she expressed shock that he was still practicing. Avoid at all costs.
I went in for a physical and he wanted me to have a heart test and other things I did not want to do at that 1st visit. I also told him my insurance does not cover yearly physicals and he told me yes it does.How would he know?? He told me that he has a few other patients like me who think they know better than him. I was very uncomfortable at this point and told him that I didn't think this was going to work out. He said your right and then started telling me how I wasted his time.I asked if I could have my paperwork back with my ss# etc and he told me to take a hike. Real piece of work. I have never been treated so poorly in my life.
I went in for an ankle sprain and the doctor wanted to run unnecessary tests without offering an explanation when asked. He took a personal call in the middle of my appointment after making me wait for nearly an hour. He was rude and verbally abusive when I asked for an explanation of the tests he wanted to run. He wanted to take blood for a sprained ankle which I still don't understand why. I left his office without receiving any care.
My experience. Not a nice man! Rude,no compassion,forgetful. Each time is like the first time there.Doesn't remember you or why you're there until he reads his notes,even then,he can confuse you with someone else as he did with me, thank God because as he told me, THAT person has some REAL problems,like mine weren't!Has a sign that reads "Yes or No, details later". Thought it was a joke until I got to know him better. Ended up feeling helpless after going to him for awhile. No wonder he tries to prescribe anti depressants when I wasn't even depressed until going to see him for an extended period of time, that has now has totally depresses me. Have just switched doctors,as I've lost ALL faith in him! When you dread going to a doctor because you know the results before even going, it's time for a change!
They need a catagory for compassion of which this doctor has none. If you want to be called fat when you are 5 pounds over weight or yelled at when you just received a diagnosis of terminal cancer this is the place to go! Otherwise anywhere is better than here.
Dr. Jehangir P. Mistry's Credentials
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- Topiwala Nat'L Med Coll, Univ Of Bombay, Bombay, Maharashtra, India (Grad. 1972)
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Residentialcommercialfamily owned and operatedindustrialno job too big or too small086840attr:commercial216280attr:commercial
Big Top Contractors IS a Complete Site Work Contractor with over 60 years of experience. Specializing in Demolition, Excavation, Land Clearing, Paving, Storm Draining and Sanitary Sewer Lines, as well as Landscaping. Service Residential, Commercial and Industrial customers. Big Top Contractors have Heavy Equipment Rentals including: Dump Trucks, Excavators, Bull Dozers and Back Hoes. We offer...
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Big Top Contractors Inc is located at 31 Elliot St in Avenel and has been in the business of Excavation Work since 1949.
Big Top Contractors can be found at Elliot St 31. The following is offered: Snow Removal. The entry is present with us since Sep 10, 2010 and was last updated on Nov 14, 2013. In Avenel there are 1 other Snow Removal. An overview can be found here.


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Looking for a medium format camera to hitch to your drone? Phase One Industrial's newest aerial camera system, the iXU 180, is being hailed as the "world's smallest" 80-megapixel medium format system designed for aerial photography.
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