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Interview with
Turtles are all around the world, but the Mother Turtle comes from Thessaloniki (Greece) and her sound is strong and beautiful enough for the sensation that has already been created. Their drummer George spreads every single detail for the band and many other things. Praise the lines...
Mesogios Sea is the home "land" of turtles and Mother Turtle, George, appeared in Greece (the land of Mesogios) suddenly and made many of us, the lovers of the prog sound have turned our eyes and ears to you. Does the band realize what has been caused?
That's great. It's great to know that your creation has turned eyes and ears into a certain style of music, especially on Prog Rock! Of course all we've ever wanted is to write music to express our feelings.nothing more.
Tell us how everything began and who Mother turtle are.
Mother Turtle begun as a jam project. The first two members was me and Kostas (guitars, vocals), then came Kostis (bass) and last but not least George (keys). From the very beginning the chemistry was there, and I mean THERE!!! Everything came up normally so we knew that this band was meant to be.
You know that nowadays, it's not something slavish for Greece to have prog rock bands. Apart from you there are also Σeptember Code, with an excellent debut and both of you have made great sense in abroad even without performing live your material. What do you accredit this to?
I think that we know damn right what we're doing! We know what we want to play, and how prog sounds good! Also in the writing process we paid attention in even the smallest detail in our music and that's something that the listener understands. This I think is the key.
Before the recordings of the debut, was the band listening to something specified or you just let your inspiration be the "flesh of your body"? Anyhow, the Fathers of prog sound that are heard in the album make it more unique rather than a cheesy emulation.
We didn't want to create something that sounds like Rush or Camel! We were composing music and expressing ourselves but of course the influence was there! I think when a musician composes, the way the song or the album sounds is a mixture of personality and influence and that's what Mother Turtle sounds like to me.
How came the thought for the ideally use of Al Pacino's monologue in "God Games"? And behind the front image of the album is there any deep message or thought about anything?
We wanted to have someone to pray to on stage so we used Al (hahahaha)
He is the ''special guest'' of the album! The front cover is our beloved surreal rhino in love (hahahaha)
If I ask you to pick your favorite song from the album, can you do it? And if yes, why this is your chosen one? As I have seen the most people choose "God Games" as the top of the album. Personally, I cannot choose. Each song has something unique.
It's maybe the hardest thing for me! I adore ''God Games'' of course, especially cause of the AMAZING guitar solo of Kostas! But every single moment of the album is part of my soul.so no, I can't choose.sorry!
The biggest part of the progsters love to collect their beloved albums on vinyls, and right now the only way listening to your album is on streaming (or by the free downloading that you offer). What are the plans for the release of the album on CD and vinyl?
We're about to release the album on CD soon enough! Then if the thing rolls and we have cash we'll release it in vinyl too.unfortunately it's very difficult financially to do all the things we want, the way we want them!
Any tour plans for this year? Or do you intend to move to your next release in order to have more material in your live shows?
Of course when the album is available on CD we'll perform it live! We're a live band after all and we can't wait to perform the album live again.it's been a while! There are thoughts about a small Greek tour but we don't have something scheduled yet.
Many of us listen to ancient masterpieces from King Crimson, Rush, Genesis, Harmonius and the list goes on. so, beyond them are there bands in prog rock from the '90s till today that the Mother Turtle members listen to and admire?
Of course there are bands that kept the prog flame alive! Porcupine Tree, Spock's Beard, Sieges Even, Sylvan, Glass Hammer and Phideaux to name a few.
George you're a drummer with a power/heavy metal backround (and you still remain). How was for you to do that different step? And I'm certain that we haven't seen your most technical side yet, right?
I don't consider myself a power/heavy metal drummer but of course I adore playing and listening to these stuff too, (especially Emerald Sun)! The biggest influence for me though is Gavin Harrison of Porcupine Tree and Neil Peart of Rush so, ''prog'' is pretty much a known territory for me! I also have a project in the making where I'll play some of the most difficult parts (technically) I have ever played.so stay tuned!
Share with us your top5 on prog rock albums of all ages.
I think the best prog rock album ever made is Mother Turtle..hahahahahahahahahaahaha
I also could write down all the Rush discography of course but here is maybe my top 5.
1) The Art Of Navigating By The Stars - Sieges Even
2) Hemispheres - Rush
3) Permanent Waves - Rush
4) In Absentia - Porcupine Tree
5) In The Court Of The Crimson King - King Crimson
This is it, thank you very much or this chance to talk about Mother Turtle. I wish you keep writing excellent music and receive as much recognition as you cannot imagine. The rest keep praising Mother Turtle. Anything last George?
I would like to thank everyone who supported this band from the very beginning but mostly Kostas Kofinas. I didn't have the chance to mention in the interview that this man was, is and will remain the fifth member of the band! His help in the whole process is priceless and without him this album would have been something different and for sure much worse! So thank you Kostas!!!
Praise The Mother Turtle!
Interview by Dr. Feelgood


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Based on feedback for the Battlefield Hardline Beta last June as well as from the Community Test Environment, Visceral, namely GM Steve Papoutsis has taken the liberty to reveal some changes that have been made following the beta.
The two factions of the game, Cops and Criminals now have faction specific weapons. RPGs and mines are now not issued to any class, instead being transformed into Battle Pickups. Papoutsis explained more on the change:
"The other big change is more around explosives and some of the heavy duty weapons. Obviously with Battlefield you have this really solid foundation of rock, paper, scissors gameplay. The heavy weapons counter the vehicles, etc. There's a great balance that the team has created over the years. We wanted to include RPGs and mines – and we did – but people felt that it took them out of the fiction a little bit; that you could just load up a character and equip an RPG. So that wasn't an easy thing to get rid of because it's part of the balance.
The design team came up with a cool ideas based on that feedback. One is to have some of those more powerful weapons be pick-ups so it creates a secondary objective. Some of the modes, like in Hotwire, the RPG cache is one of the objectives. If you control that cache your team has access to the RPG. And that creates more interesting gameplay. We also came up with the notion of 'junk in the trunk' – what if we allowed players to equip special items to their vehicle instead of their inventory loadout. It goes back to movies where bad guys pop open the trunk to grab something big. When you play the game, if you set up your vehicle loadout to have a specific weapon in the trunk and you control the vehicle you can use that item. So now you want to get a vehicle to get access to a specific weapon, which also fits the fiction better."
The Game's open beta was stated for release in Fall 2014 but following the game's delay, it will most likely occur in early 2015. -- awyman13 Talk Work 16:32, October 19, 2014 (UTC)
Latest News...
Hello Battlefield Wiki! This is 3RDRANGER bringing you some later-than-new news about the upcoming game in the Battlefield series, Battlefield 1.
The gam…Read more >
GREAT NEWS Battlefield Wiki. DICE has finally announced in a blog post that the Open Beta for Battlefield 1 will be available for everyone on August 31st…Read more >
ORIGINAL POSTRead more >
Late last week, DICE revealed that they'll be unveiling a brand new Battlefield game on Friday, May 6, with a countdown clock on the offi…

subset: commoncrawl


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I had a simple mastectomy 2 1/2 years ago and a breast reduction and lift on the remaining breast.
Doctor Answers 2
Pain in Breast After Simple Mastectomy and Reconstruction
It is quite normal to have pain after your reconstruction but in light of the fact that this is now occurring 2 1/2 yrs after your procedure may be of some concern. I would suggest that you visit with your reconstructive surgeon to discuss your concerns. The most likely cause would be some scarring around the implant which could be contributing to your discomfort. Good Luck
Learn more: http://www.ruckermd.com/content/procedures/breast-reconstruction/revision-after-breast-reconstruction-reconstruction-after-lumpe
Breast reconstruction and pain
Yes, it is a good idea to see your plastic surgeon if you are having new onset pain after reconstruction.
Learn more: https://www.stevenwallachmd.com/breast/breast-reconstruction/
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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Community Mental Health offers walk in or call crisis 24 Hours a Day (Walk-in services are for those in immediate crisis and cannot wait for an appointment). Visit our Walk-in Clinics Map to get directions to your nearest CMH Building. Crisis services provides crisis intervention, assessment, and screening for voluntary and involuntary psychiatric hospitalization, or learn the four signs of mental crisis.
Crystal Palmer, Director of Children's Initiatives at Detroit Wayne Mental Health Authority, earned her Bachelor's in Social Work from Saginaw Valley State University, in Saginaw MI and subsequently a Master's in Social Work from Wayne State University, in Detroit MI. Ms. Palmer has spent the past 15 years working with at-risk children and their families in Wayne County, MI.
In her current role, Mrs. Palmer is responsible for the oversight, management, development and coordination of services for children and youth with Serious Emotional Disturbance (SED) and their families within Wayne County's public mental health system. She also acts as the Principal Investigator for a Substance Abuse Mental Health System Administration System of Care Expansion Grant.
Detroit Wayne Mental Health Authority provides a comprehensive and integrated array of services/supports which inspires hope and promotes recovery/self-determination. Children, youth and families with co-occurring mental health, substance abuse and physical health conditions receive services within a system of care that is:
Community based: with the focus of services as well as system management resting within a supportive, adaptive infrastructure of structures, processes, and relationships at the community level.
Family-Centered: families have a primary decision making role in the care of their own children as well as the policies and procedures governing care for all children in their community.
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Cultural and Linguistically Responsive: organizations, programs, and services are relevant and unique to each individual and family's cultural, linguistic, and social needs.
Trauma Informed: when organizations, programs, and services are based on an understanding of the vulnerabilities or triggers of trauma survivors that traditional service delivery approaches may exacerbate, so that these services and programs can be more supportive and avoid re-traumatization.
Children's Initiatives: programs, services and/or projects that being implement within the network of care that show "evidence" and/or "promise" of good outcomes for children with SED. These services are:
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Transmission electron microscopy characterization of fluorescently labelled amyloid β 1-40 and α-synuclein aggregates
Fluorescent tags, including small organic molecules and fluorescent proteins, enable the localization of protein molecules in biomedical research experiments. However, the use of these labels may interfere with the formation of larger-scale protein structures such as amyloid aggregates. Therefore, we investigate the effects of some commonly used fluorescent tags on the morphologies of fibrils grown from the Alzheimer's disease-associated peptide Amyloid β 1-40 (Aβ40) and the Parkinson's disease-associated protein α-synuclein (αS).
Using transmission electron microscopy (TEM), we verify that N-terminal labeling of Aβ40 with AMCA, TAMRA, and Hilyte-Fluor 488 tags does not prevent the formation of protofibrils and amyloid fibrils of various widths. We also measure the two-photon action cross-section of Aβ40 labelled with Hilyte Fluor 488 and demonstrate that this tag is suitable for use with two-photon fluorescence techniques. Similarly, we find that Alexa Fluor 488 labelling of αS variant proteins near either the N or C terminus (position 9 or 130) does not interfere with the formation of amyloid and other types of αS fibrils. We also present TEM images of fibrils grown from αS C-terminally labelled with enhanced green fluorescent protein (EGFP). Near neutral pH, two types of αS-EGFP fibrils are observed via TEM, while denaturation of the EGFP tag leads to the formation of additional species.
We demonstrate that several small extrinsic fluorescent tags are compatible with studies of amyloid protein aggregation. However, although fibrils can be grown from αS labelled with EGFP, the conformation of the fluorescent protein tag affects the observed aggregate morphologies. Thus, our results should assist researchers with label selection and optimization of solution conditions for aggregation studies involving fluorescence techniques.
Fluorescent tags are commonly used to monitor proteins and peptides in microscopy and spectroscopy experiments
In this Paper, we present TEM and other characterizations of fluorescently labelled Aβ40 peptide and αS protein. Aβ is associated with Alzheimer's disease, while αS is linked to Parkinson's disease; therefore fluorescently labelled αS/Aβ constructs may be useful for understanding the initiation and progression of these common human neurodegenerative disorders. Indeed, fluorescently-labelled Aβ and αS constructs have been used in numerous studies of protein interactions, trafficking, and degradation, as well as in investigations of structural changes linked to amyloid aggregation (see articles referenced in
High-resolution imaging techniques, including TEM and atomic force microscopy, enable identification and classification of aggregates, which may include protofibrils, amyloid fibrils containing varying numbers of strands, and amorphous aggregates. In contrast, methods used to quantify fibril production, such as thioflavin-T binding, light scattering, Fourier transform infrared spectroscopy, and circular dichroism spectroscopy, cannot discriminate among different types of β-sheet rich species
We examine Aβ40, peptides tagged with three extrinsic fluorophores (AMCA, TAMRA, and Hilyte Fluor 488). These labels were selected because their emission peaks are reasonably well-separated, making them potentially useful for multi-channel imaging or fluorescence cross-correlation spectroscopy applications. In addition, peptides tagged with these dyes are readily available from commercial sources.
For αS, we compare small organic dye (Alexa Fluor 488) with fluorescent protein (EGFP) labelling. Alexa Fluor 488, EGFP, and Hilyte Fluor 488 have similar excitation and emission spectra, and therefore are compatible with similar optical systems. In addition, investigations of the effects of fluorescent protein tags are particularly important given the high potential value of these tags for
Our TEM images show that several extrinsic fluorescent labels do not preclude the growth of multiple fibril varieties for Aβ40 and αS. In contrast, we observe two distinct types of rigid aggregates when αS-EGFP solutions are incubated near physiological pH. Moreover, disruption of the EGFP tag results in the growth of additional species. Therefore, although the fluorescent protein label does not prevent aggregation of the αS-EGFP construct, the fibrillization pathway is affected by the conformation of the EGFP tag. We believe that our images will provide a starting point to assist researchers in fluorophore selection for aggregation studies, although additional characterizations will be necessary for some applications.
Extrinsic Dye Labelled Aβ40 Aggregates
Figure 1
TEM images of aggregates grown from Aβ40 labelled with three extrinsic fluorescent dyes
TEM images of aggregates grown from Aβ40 labelled with three extrinsic fluorescent dyes. Each column corresponds to one fluorophore. The scale bar is 200 nm wide and all images are shown at the same magnification. Unless otherwise noted, the samples were incubated at 22°C under quiescent conditions. The protein concentrations, incubation times
For the TAMRA and Hilyte Fluor 488 labelled Aβ40 samples shown in Figure
Potential applications of fluorescently labelled peptides include two-photon imaging and two-photon fluorescence correlation spectroscopy. The two-photon action cross-sections of AMCA and TAMRA are sufficient for these applications
Figure 2
Two-photon action cross-section of Hilyte Fluor 488 labelled Aβ40
Two-photon action cross-section of Hilyte Fluor 488 labelled Aβ40. The two-photon action cross-section (ϕfσ2p) of Hilyte Fluor 488 labelled Aβ40 is compared to that for Alexa Fluor 488 free dye. The units for ϕfσ2p are Goeppert-Mayer (GM), where 1 GM = 10-50 cm4 s photon-1.
Alexa-488-αS Aggregates
When aggregates grown from αS variant proteins labelled with Alexa Fluor 488 are imaged using TEM, we detect both rigid fibrils (Figure
Figure 3
TEM images of aggregates grown from αS variants labelled with Alexa Fluor 488
TEM images of aggregates grown from αS variants labelled with Alexa Fluor 488. The scale bar is 200 nm wide and all images are shown at the same magnification. All samples contained 10 mM pH 7.5 NaPhos, and unless otherwise specified, the solutions were incubated at 37°C with shaking. The protein concentrations, incubation times
For human wild-type (WT) αS, N-terminal (position 9) and C terminal (position 130) labelling is compatible with growth of both fibrils types (Figure
Note that our images do not enable direct comparisons of aggregation properties of proteins labelled at the C vs. N termini because of variability in solution conditions and incubation times, as well as the possible presence of oligomeric species in the stock solutions. Therefore, we are only able to observe that certain structures are not precluded by the label, but cannot determine whether a label or labelling location may tend to favour a particular type of aggregate. More detailed studies are necessary to determine whether labelling has any subtle effects on aggregation pathway selection.
EGFP-αS Aggregates
We observed fibrils when samples containing 75 μM and 150 μM αS-EGFP in PBS were prepared using unfiltered αS-EGFP stock solutions (Figure
Figure 4
TEM images of aggregates grown from seeded or unfiltered αS-EGFP stocks
TEM images of aggregates grown from seeded or unfiltered αS-EGFP stocks. The scale bar is 200 nm wide and all images are shown at the same magnification. Unless otherwise specified, all solutions contained PBS buffer (10 mM pH 7.5 NaPhos, 150 mM NaCl) and the samples were incubated with shaking at 37°C. The protein concentrations, incubation times
Additional images of αS-EGFP fibrils gown from seeded or unfiltered solutions at pH ~7.5 in various buffer conditions are shown in Figure
When αS-EGFP solutions containing fibrils were examined by eye, they appeared uniformly fluorescent, unlike the extrinsic fluorophore-labelled αS samples in which fluorescent aggregated material was clearly visible at the bottom of the tubes. This may be a result of αS-EGFP fibrils remaining suspended in solution, or the fibril fraction may be a minor component of the sample. Alternatively, the EGFP tag may be quenched or altered in the αS-EGFP fibrils
Fluorescent protein tertiary structure can be disrupted by extremes of pH
Figure 5
Spectral properties of the αS-EGFP construct
Spectral properties of the αS-EGFP construct. (A) Absorbance spectra of αS-EGFP (dashed lines) and EGFP (solid lines). Spectra are shown for 5 μM protein in 10 mM phosphoric acid (pH 2.4, red lines) and 10 mM NaPhos buffer (pH 7.5, black lines). (B) The normalized fluorescence emission from αS-EGFP (squares) and EGFP (circles) as a function of TFE concentration. The signal from 0.3 μM protein is measured after a 2.0 ± 0.5 minute incubation at 22°C (cyan symbols), after 2.0 ± 0.5 minutes at 37°C (red symbols), and after 24 ± 2 hours at 37°C (yellow symbols). The error bars reflect the standard deviations of measurements of three identical samples and baseline uncertainties.
Figure 6
TEM images of αS-EGFP aggregates grown in potentially denaturing conditions
TEM images of αS-EGFP aggregates grown in potentially denaturing conditions. The scale bar is 200 nm wide and all images are shown at the same magnification. All samples were incubated for three weeks, and unless otherwise specified, the incubation temperatures were 37°C. The protein concentrations and other solution conditions are as follows: (A-B) 50 μM αS-EGFP at pH 2.4. (C) 50 μM αS-EGFP at pH 2.4 with 150 mM NaCl. (D-E) 50 μM αS-EGFP in pH 7.5 NaPhos with 15% TFE. (F) 50 μM αS-EGFP in pH 7.5 NaPhos with 10% TFE, after incubation at 22°C under quiescent conditions. (G) Same as F, but the solution contained 75 μM αS-EGFP. (H) Same as F, but the solution contained 15% TFE. (I) 50 μM αS-EGFP at pH 2.4 with 15% TFE. (J) 50 μM αS-EGFP at pH 2.4 with 15% TFE and 150 mM NaCl.
In this Paper, we have demonstrated that several small extrinsic fluorescent tags are suitable for use in Aβ40 and αS aggregation studies. We show that the AMCA, TAMRA and Hilyte Fluor 488 labels do not preclude the formation of many types of amyloid and fibrillar aggregates for Aβ40 (Figure
In contrast, we find that EGFP labelling of αS favours the formation of two types of rigid aggregates when protein solutions are incubated near physiological pH (Figure
Interestingly, we are able to grow fibrils from αS-EGFP in solutions in which EGFP tertiary structure is likely disrupted (Figure
We have presented TEM images and other characterization of aggregates composed of fluorescently labelled proteins. Our images support the use of several small intrinsic fluorescent tags in studies of αS and Aβ40 aggregation. In addition, we present images of fibrils grown from αS labelled with EGFP. Near neutral pH, αS-EGFP fibrils are rigid and often feature an indistinct helix wound around a rigid core. Acid or TFE denaturation of the EGFP tag results in the formation of additional types of αS-EGFP aggregates. Our images may assist researchers in the selection of labels and in optimization of experimental conditions for protein aggregation studies involving fluorescence techniques.
Solutions and Reagents
MilliQ or HPLC grade water was used to prepare all solutions. Sodium phosphate (NaPhos) buffer salts were purchased from Sigma. Trizma brand pre-set pH 7.7 crystals (Sigma) were used to prepare Tris buffers that were pH ~7.4 at 37°C. Temperature-dependent changes in the pH of other buffer solutions were ignored. Acros Organics brand 99.8% pure TFE was purchased from Fisher Scientific. Sodium azide (Sigma) at ~0.02% w/v was added to all solutions incubated at ~20°C for over 24 hours. A bench-top orbital shaker operating at 200 RPM was used to agitate some samples during incubation.
Aβ40 Solubilization
The three Aβ40 peptide tags discussed here are Hilyte Fluor 488, TAMRA (5-carboxytetramethylrhodamine), and AMCA (7-Amino-4-methylcoumarin-3-acetic acid). N-terminally fluorophore labelled, synthetic Aβ40 peptides were purchased from Anaspec. The percentage of singly-labelled Aβ40 peptide was measured by the manufacturer using HPLC and mass spectrometry, and was reported as > 90% for all batches examined in this Paper.
Protein stock solution preparation roughly followed the protocol in Bitan and Teplow
Alexa Fluor 488 Labelled αS Preparation
Alexa Fluor 488 was purchased from Invitrogen, and labelling was generously performed by Trudy Ramlall and Prof. David Eliezer of Weill Cornell Medical College using previously described procedures
We estimated the dye-to protein ratio (
Using equation 1, we obtained
αS-EGFP Expression
Vectors for mammalian expression of the human WT αS-EGFP construct were a kind gift from Prof. Bradley Hyman of Massachusetts General Hospital Medical School at Harvard University; information about this construct can be found in McLean, et al.
Plasmid DNA was purified with the Qiagen Miniprep kit.
Site-directed mutagenesis was performed on the provided plasmid to introduce a 6xHisTag at the C-terminus of the αS-EGFP fusion protein. The primers used for mutagenesis were: 5'-GGC ATG GAC GAG CTG TAC AAG CAC CAT CAC CAC CAT CAC-3' and 5'-CTA GAG TCG CGG CCG CTT TAG TGA TGG TGG TGA TGG TGC TT-3'. After transformation, colonies were screened for the presence of the HisTag by PCR using the following primer pair: 5'-GGG ATC CAT CGC CAC CAT GG-3' and 5'-CGC GGC CGC TTT AGT GAT GG-3'. A plasmid which screened correctly was verified by sequencing. The final construct was based on the cloning vector EGFP-N3, with αS fused to the N-terminus of EGFP and a 6xHisTag fused to the C-terminus of EGFP.
The fusion construct described above was moved into a vector for
Plasmids were transformed into BL21Star (DE3) cells (Stratagene) harbouring the pRARE2 plasmid (Novagen) and selected on kanamycin/chloramphenicol media at all stages. Protein expression in shake flasks was performed as described in the pET-system manual, with induction by IPTG (1 mM) at reduced temperature (15°C) and overnight incubation post-induction. Cells were harvested by centrifugation, lysed by sonication, and HisTagged protein was purified on 5 mL HisTrap HP columns (GE) using an AKTA FPLC. Buffers used for purification were A) Binding: 20 mM Tris, pH 8.0, 500 mM NaCl, 30 mM Imidazole. B) Elution: 20 mM Tris, pH 8.0, 500 mM NaCl, 500 mM imidazole. The column was washed with A until the
αS-EGFP Dialysis and Buffer Exchange
To prepare the samples shown in Figure
For the samples shown in Figure
Fluorescence Spectroscopy
Fluorescence emission spectra for 480-580 nm were collected using 460 nm excitation via a QuantaMaster fluorescence spectrofluorometer (Photon Technology International). All fluorescence emission signals were normalized to the emission signal from EGFP in PBS (10 mM NaPhos, 150 mM NaCl) at room temperature (22 ± 3°C). Correction for lamp fluctuations was automated by the vendor-supplied software. For the 37°C samples, the temperature was maintained during fluorescence measurements using a NesLab Endocal RTE-110 chiller/circulator (Thermo Scientific). In each case, three identical samples were measured and their standard deviations calculated in order to determine the measurement variability.
Determination of Protein Concentrations
UV or visible light absorbance measurements via a double-beam Cary-300 spectrophotometer (Varian) were used to quantify the amount of protein in the stock solutions. The absorbance of the fluorophore was measured and concentration calculations were performed using manufacturer-supplied extinction coefficients for the organic dyes and ε = 55,000 M-1 cm-1 for EGFP
Transmission Electron Microscopy Imaging
The general procedure for the TEM sample preparation and imaging is described in Anderson, et al.
Two-Photon Action Cross-Section Measurements
A pulsed titanium sapphire Mai Tai laser (Spectra Physics) was used to excite the fluorophores over the wavelength range of 760-1000 nm. The excitation and emission light was focused through a 63x, 1.2 NA water immersion C-Apochromat objective lens (Zeiss) into ~100 nM samples, which were mounted on an inverted microscope (IX71, Olympus). The intensity of the excitation beam was measured using a photodiode, while the intensity of the emitted fluorescence was detected using a gallium arsenide phosphide photomultiplier tube (Hamamatsu). Linear fitting to the emitted light vs. incident intensity squared curves was performed at each measured wavelength and the resultant slopes were normalized to the values for a pH 11 Fluorescein standard
Authors' contributions
VLA and WWW designed research and analyzed data. VLA carried out all experiments and wrote the paper. All authors read and approved the final manuscript.
Prof. Bradley Hyman at Massachusetts General Hospital Medical School at Harvard University generously provided vectors for mammalian expression of the αS-EGFP construct. Transformation into a bacterial vector and subsequent protein expression was performed by Dr. Cynthia Kinsland. Trudy Ramlall of the Eliezer lab at Weill Cornell Medical College kindly labelled and expressed αS variants tagged with Alexa Fluor 488.
This work made use of the electron microscopy facility of the Cornell Center for Materials Research (CCMR) with support from the National Science Foundation Materials Research Science and Engineering Centers (MRSEC) program (DMR 1120296). Funding was provided by grants from the National Institutes of Health (5 R21 AG026650) and the National Science Foundation (STC program under agreement No. ECS-9876771). Some of this material was based on work supported under a National Science Foundation Graduate Research Fellowship. Any opinions, findings, conclusions, or recommendations are those of the authors and do not necessarily reflect the views of the National Science Foundation.
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Designing conditions for in vitro formation of amyloid protofilaments and fibrils
Preparation of aggregate-free, low molecular weight amyloid-beta for assembly and toxicity assays
Quantification of alpha-synuclein binding to lipid vesicles using fluorescence correlation spectroscopy
Rapid Fluorescein and Protein Assay Method for Fluorescent-Antibody Conjugates
Conformational properties of alpha-synuclein in its free and lipid-associated states
Mechanisms of quenching of Alexa fluorophores by natural amino acids
Dual-color flow cytometric detection of fluorescent proteins using single-laser (488-nm) excitation
Multiphoton excitation cross-sections of molecular fluorophores


text:
by: gvitmv • 6
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by: gvitmv • 6
Do you want to see different molecules such as adrenaline, cocaine, amino acids and etc. with real 3D stereoscopic mode?
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MolView helps you to understand space structure of complex molecules.
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Some people can see stereoscopic image without special glasses using Side by Side or Mirror separated mode.
You can simply open any file in PDB(protein Data Bank) format or choose some molecules in the available list and interact with it by simple standard touch control.
File browser shows files only with *.pdb, *.ent, *.txt extensions and only not empty directories.
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Go head-to-head with Ad Land's finest in the
world's first Ping-Pong Hangout Tournament.
World's first? You haven't heard that since your last award
video. But here it's true. Take on your rivals in this global
interagency smackfest, and let their paddles score
millions of hits (like their recent campaign didn't).
Agency scores shown are comprised of the cumulative wins of the
individual agency employees who independently played the game.
Games Played
Warning: Invalid argument supplied for foreach() in /home/pingpong_admin/pingpong.mystagingcentral.com/index.php on line 169
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What's this all about?
What exactly is a Hangout?
Hangouts let you have face-to-face conversations online with
up to 10 people at once. Think in-person chats, but without
the physical effort, traffic, pollution and general fuss of
moving from one place to another.
So it's just a video chat?
Not quite, but you're headed in the right direction. Hangouts can be small, like a video
chat, or huge, broadcasted to thousands across the globe. When you broadcast your
Hangout, it's also recorded and automatically posted to YouTube for you to share.
What's this Hangout app business all about?
In a word, magic. The Hangouts API allows you to create custom experiences for Hangouts.
A few are prebuilt into Hangouts—like the ability to share your screen or watch YouTube
videos together. But you can create your own too.
Here's what you need to know.
The basics of Google+ Hangout apps
A Google+ Hangout app behaves like a normal web app, but
with the addition of rich, real-time functionality provided by
the Google+ Hangouts API. Anything you can put in an iFrame
you can add to a Hangout app, since it's just an iFrame inside
a Google+ Hangout. You have full control of the iFrame
content, but the Hangout user interface stays constant.
Think of the wild possibilities.
Did you know...?
- • The average duration of a Hangout increases by 2.5 times when participants use an app
- • One in every three Hangouts uses an app
Ready to get started?
Check out our getting-started guide for the Hangouts API.
What can you do with the
Google+ Hangouts API?
Make it physically interactive.
Let users control the app with their bodies.
Collaborate or play in real time.
With synchronized data across all Hangouts in an app,
you can draw, play, sketch, share and whiteboard as if
you were in the same conference room, man cave or
basement rec room together.
Create custom media effects.
Media effects allow you to "stick" any image to a user's head as
they move around the screen in the Hangout. It's not just for
funny hats or moustaches or reindeer antlers (though we love
'em). Anything you can put on someone's head you can
create here. And imagine branding these digital accessories
with your logo.


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Last week Pakistan suffered a horrible Islamic Taliban terrorist attack on a school full of students. 132 students and staff were killed, making this one of the worst Taliban attacks in the Pakistani city of Peshawar.
(See More: 132 Children Dead In Terror Attack On School, Tehreek-e-Taliban Takes Credit)
Despite being a Muslim hotbed, Pakistan has a substantial Christian population that lives in danger most of their day due to their beliefs. And they have been stepping out in heroic efforts to protest the mass killing at the Peshawar school. Taking to the streets and holding signs that say "United We Stand" and other uplifting phrases.
A few days ago, however, protesters found a way to really zing Muslims and Taliban members into a state of fury. A big group of the protesting Christians suited up for the occasion, literally.
As you can guess, Santa is a big symbol of Christianity to die-hard Muslims (which is most of Pakistan) and the bravery these Christians took in celebrating their beliefs while standing up for their right to believe it was a huge single-digit salute to the Taliban. A beautiful present just in time for Christmas.
The Pakistani Christians were expecting the US to step in, but Obama issued a shameful White House statement, completely downplaying the fact that this attack was caused by radical Islamic terrorist group Taliban. They do, however, have support of some military commanders on Pakistan soil who deployed drone fleets to take out a few of the attackers.
(h/t: Conservative Tribune)


text:
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This screensaver shows on your screen 3D clock (time format HH:MM:SS) rotating.
Use this security logo:
Freeware is computer software that is available for use at no cost or for an optional fee.
Tutorial not found. Let know us about any useful video tutorial.
screensaver, clock, time, ball
New Release
You may include this program on any CD compilation.
BallClock 3D ScreenSaver is freeware.
You may freely distribute it without modification of original package.


text:
On 10/05/2010 04:07 AM, Eric Blake wrote: <snip>
Hmm; the nested case $host seems odd to me. Maybe a better layout is: with_linux=no with_osx=no case $host in *-*-linux*) with_linux=yes ;; *-*-darwin*) with_osx=yes ;; esac if $with_linux = no; then if test "x$with_lxc" != xyes ... fi
Ahhh, that's a much better idea. Will do. :)
AC_ARG_WITH([network], AC_HELP_STRING([--with-network], [with virtual network driver @<:@default=yes@:>@]),[],[with_network=yes]) -if test "$with_libvirtd" = "no" ; then + +dnl theres no use compiling the network driver without the libvirts/theres/there's/
Oops. Thanks. :)
+dnl daemon, nor compiling it for MacOS X, where it breaks the compile + +if test "$with_libvirtd" = "no" || test "$with_osx" = "yes"; then with_network=no fiMostly looks okay, but probably worth a v2 to make sure the $host-detection case statement rewrite is still good.
No worries. It'll be a tomorrow job though. :)


text:
Living With Aspergers: Help for Couples
30 Relationship Tips for Aspergers Men
Living With Aspergers: Help for Couples
12 comments:
For me communication is important. A constant communication will make a couple more comfortable with each other. This is also the time when you get to know the likes and dislikes of each one of you.ReplyDelete
I don't know who wrote this, but so many of these things would have meant the world to me if my Asperger's husband had done even a few of the suggestions on this list. What I wonder, though, is if any Asperger's men actually read this list, or if they are willing to do any of these? My husband's so clear about how important his interests and opinions are, that he really cannot envision the importance of anything that matters to me or our children. I have finally accepted that he is just unable to think outside himself - but it has left me feeling completely neglected and alone. As long as I leave him alone to do whatever he pleases, while I take care of our home and our children, then we get along fine. But I can't do it all anymore.ReplyDeleteReplies
Dear Dream to Dance, I myself could have written exactly what you have written. My daughter has Aspergers as well as my husband , I really have had enough of being alone in this marriage and long to be loved and taken care of. My family have written me off now, so I have nowhere to go.Why is there no support for us?Delete
Hi, This could have been me that wrote your blog, plus I have a daughter as well with Aspergers, so my life is well and truly serving everyone else around here and not even being appreciated or noticed.Why is there no help for us?Delete
I would love a hug or someone to notice my pain and sadness.
*Virtual hug*
Christ, that sounds difficult. There's not too much I can say, but you have wonderful strength and a truly brilliant personality to endure so well. I am Sperg myself, but I'll try to advise as best I can. This is going to sound cynical, but to get him to do things, you'll have to make it his while.
How I agree with you. Presents? Nights out? Doing something without being asked? Empathy?. There are some good traits, but over the years my appreciation of them has been worn down. I make all the effort and all the compromises, I try to predict the flash points, and circumvent them by pandering to him. Initially he did make an effort but as soon as the objective was reached there was was no further point. There is no relationship or sense of fulfillment. I feel trapped because I know it is not his fault, and it would be cruel to leave.Delete
Oh my gosh this is almost word for word what I have been saying in my head.Delete
My partner of five year refuses to communicate with me and is only about himself.
After five years I've gone around in circles listening to him push me away saying how tired and drained he is 24/7 .
He would never read this list and take action. What he might do is listen to me read it out loud then respond with "I will never do that for you. Find someone who will."
The truth is I did date other guys and they had their own bag of misery too. "Normal men are "simple" they make a lot of mistakes, but at least they talk and communicate and have the capacity to be emotional.
Oh well, maybe my life lesson on earth is to be miserable with a man who wont ever talk to me and learn how to get over my needs or something.
Every page like this is help for both sides. I just cannot understand how any of us seeking direct phrases or things we can do to make it easier are supposed to feel there is any point when every mate describes how awful we are. It makes me think leaving would be a sign that I care...ReplyDelete
I feel your pain Emma Moorby! The only thing that helps me is my relationship with the ALL MIGHTY! My faith in Him fills the gaps left by my husband. Also, I try to follow the teachings of Jesus which, though difficult, helps a lot. I hope that gives you some hope! ;)ReplyDeleteReplies
perhaps you could take a look at the work of Maxine Aston who seems to have a way to inroad sanity and salvation into being the partner in these situations ... she has a web site and some research books/papers to help .. based in the UK mind you.Delete
So true. I believe no one can truly satisfy all the needs of the other person, except the Lord. God bless u:)Delete
No 5. Don't go outside the marriage. Oh how that hurts! My husband isn't diagnosed however having completed Prof Baron Cohen's questionnaire (Cambridge expert, UK)he has strong indicators of being on the autistic spectrum. Second marriage - step family both sides, lots of stress and rather than work with me, he turned away, couldn't cope, said he didn't know what to do, was flattered by a woman and as a distraction he had an affair on a business trip to the USA which I discovered having a sense that something was wrong. It is unbearable. He cannot cope with conflict, goes silent and withdraws in the face of my unhappiness and initially anger. He couldn't cope with Counselling - didn't know at the time but suspected his issues were asperger based. He won't do anything to help himself, occasional 'I love you', pat on the back and a hug. If I'm compliant it's ok but when I'm unhappy and challenge him he withdraws. I've had to research and work on healing myself. He thinks he has made changes but not sustained ones. It's not a relationship. If I suppress everything and keep it superficial, he'd be okay with that. I've read/researched about Aspergers and got him to read a book but that is as far as he has gone. Everything I read states that the NT has to be the one modifying and adapting. It's too much to be betrayed and then have to dance around his sensitivities. I've hung on for 2.5 years but am nearing the end of my efforts. Any suggestions?ReplyDelete


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|"William A. Rowe Jr." <wr...@rowe-clan.net>
|Re: planning to T&R apr-util 1.3.11 later today
|Thu, 14 Apr 2011 16:54:11 GMT
On 4/14/2011 10:32 AM, Jeff Trawick wrote: > Speak up if you're working on something for apr-util. > > I had originally planned to T&R this at the same time as apr 1.4.3, > but I may as well get started. If folks want to wait for apr 1.4.3 to > test apr-util, that's fine too. > > I still anticipate that apr will be ready for T&R of 1.4.3 within a > couple of days ;) Do you mean, shipping apr_crypto? Ugh... I have a laundry list of issues that were never addressed, misfactored argument lists, unnecessary function args, pointers to pointers to pointers where nested structures would have worked - and nobody else has really even stepped up with their assessment of the overall design. I don't care if it is missing desired features, but do care if the features that are implemented now are poorly thought out. Essentially, it's a mess. I understand it is derivative of some related design flaws in apr_dbd etc, but that isn't a reason to propagate them. Attached for your perusal is my very first draft edits of the function prototypes, obviously this does very little good without all of the updates to the common and crypto provider specific structures. I had not reordered the arg lists in the .h file, nor done a second pass to correct my own work, since I'm paying more attention to apr, trunk issues, vc 10 and win64 issues at this moment. So I'm presenting this only as an illustration of how much work should happen before I'd give this API a hearty +1. I have some of this work done, but I'm not going to be in a position of committing it in the couple of weeks. If nothing else, I can at least promise to dedicate time to complete and commit my work during the May f2f in Wicklow. If what you meant to type was "apr-util 1.3", I see no reason not to T&R that immediately :)
|View raw message


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Tesla Needs An EPS Beat To See Some Upside Post Q2 Results
- Delivery targets were missed during the second quarter due to shipments in transit.
- Consensus estimates show a loss of $0.54 on revenues of $1.65 billion..
- Company outlook is positive on subsequent quarter deliveries and 2020 production targets.
Tesla (NASDAQ:TSLA) is expected to report second quarter earnings on August 3, after market close.
2016 has been a mixed bag for Tesla. After the roaring success marked by the launch of Model 3 that notched up an unprecedented 400,000 reservations within a month, Tesla missed delivery estimates during first the quarter and missed it again during the second quarter, and had to revise their 2016 sales target to 79,180 cars - lower than the previously expected range of 80,000 to 90,000 cars.
After the wild reception the Model 3 got worldwide, Tesla started ramping up its production, setting an ambitious goal to make 500,000 cars by 2018, a full two years earlier than the previous target of 500,000 cars by 2020. Now, Tesla wants to build 1 million cars by 2020. Essentially Tesla has doubled up on its expansion plans. But unfortunately for Tesla, the ramp up hasn't really gone well so far.
Deliveries in Q2
Tesla Motors, Inc. delivered 14,370 vehicles in the second quarter, (9,745 Model S vehicles and 4,625 Model X vehicles), falling far short of their estimated 17,000 cars for the period. Tesla says that 5,150 cars are on the way to the clients and now expects to deliver them during early third quarter. The downward revision of targets has caused analysts to adjust their models, and earnings estimates for second quarter are a bit all over the place, with analysts swinging from one end to another.
Consensus Estimates
JP Morgan expects the company to report a loss of 60 cents per share instead of the previously estimated 45 cents loss per share. The company reiterated its under-weight rating for the stock, and slashed its target price from 185 to 180. Tesla is now trading at the $230 level, and the stock is down by 4.17% since the start of this year. Morgan Stanley revised its 2016 EPS estimates for Tesla to -0.02 cents per share from the previously estimated $0.68 cents per share and expects the company to miss its 79,180-unit target and deliver only 70,000 cars for the year.
The consensus Q2-2016 estimate is a loss of 54 cents per share on the back of $1.65 billion in revenue. It is safe to say that production and delivery problems have pushed a lot of analysts to correct their expectation downwards.
Since the second quarter sales numbers are already out, Investors will be looking to hear updates on the Gigafactory, progress on production ramp and the outlook for the rest of the year. If the company comes out with earnings in line with estimates and gives a positive outlook for the second half of the year, the stock may see some upside.
Source: Tesla stock price chart by amigobulls.com
Delivery Guidance for Second Half of 2016
According to a company press release on July 3, this is their delivery estimate for the remainder of the year:
"With continued productivity improvements, Tesla expects output to reach 2,200 vehicles per week in Q3 and 2,400 vehicles per week in Q4. Current order rate trends and backlog support production at those levels. In total, Tesla expects to produce and deliver about 50,000 vehicles during the second half of 2016, approximately equal to all of 2015."
Tesla Stock Outlook
Deliveries and EPS are the key drivers of Tesla stock movement. Despite lower price targets, the stock is still trading at the $230 level. Any miss in earnings could push it further down. Having missed their delivery target for the second quarter, Tesla's only chance of retaining current levels is to meet or beat estimates on EPS.


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Browse All Belarusian Recipes: Belarusian Appetizers | Belarusian Soups | Belarusian Salads | Belarusian Vegetarian | Belarusian Meat Dishes | Belarusian Snacks | Belarusian Desserts
The variety of Belarusian soup recipes is quite amazing, however, many of them are just known on a local level and cannot be considered representative for the whole territory of Belarus. Here are a few Belarusian soups that have made it beyond Belarus' borders and can be found in many restaurants all over the world. A common soup in Belarus is shtchi. Made from sauerkraut, potatoes and fried onions, it is usually served with sour cream. Belarusian borshch, a soup made with beetroot, is served hot with sour cream.
Pages in category "Belarusian Soups"
The following 13 pages are in this category, out of 13 total.


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Four-piece metal herb grinders are useful for the blending of dry herbs before vaporization. While two-piece herb grinders feature a lid and container, four-piece herb grinders also feature a bottom compartment which captures the ground herb. Most four-piece metal grinders feature pollen screens that capture even the smallest bits of herb. Metal grinders for herb are exceptionally durable, withstanding years of use without wearing down.
Herb grinders finely blend herb to ensure the material is easier to vaporize. This allows for heat to pass through the vaporizer more thoroughly in convection vaporizers, and makes it easy to stir the herbs in the heating chamber of conduction units.


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September 12, 2010
As you can tell from the previous posts from today, OLD SCHOOL SUNDAYS have returned to The305.com. Atleast on a trial basis. If I get a good response and good feedback from this week's Old School Sunday posts then I'll keep doing them.
REQUESTS – Just send over your requests and feedback to firstname.lastname@example.com. And requests don't have to be hip-hop specific or from Miami. They can be from any genre and from any year. Holla.
CategoriesMiami History


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- dizzy
I. adjective
(dizzier; -est)
Etymology: Middle English disy, from Old English dysig stupid; akin to Old High German tusig stupid
Date: before 12th century
1. foolish, silly
a. having a whirling sensation in the head with a tendency to fall
b. mentally confused
a. causing giddiness or mental confusion <dizzy heights> b. caused by or marked by giddiness c. extremely rapid <prices climbing at a dizzy rate> • dizzily adverb • dizziness noun II. transitive verb (dizzied; dizzying) Date: 1501 1. to make dizzy or giddy 2. bewilder <disasters that dizzy the mind> • dizzyingly adverb
New Collegiate Dictionary. 2001.


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Battling Star Wars Drones
Emperor, you spoil us... Following on from the massively successful launch of the BB-8 Sphero Droid last year, RC specialists Propel are set to unleash a set of 4 amazing battling Star Wars quad drones. Fans young and old will finally be able to take control of iconic Star Wars vehicles in dogfights against other pilots at speeds of up to 25mph / 40km/h.
You'll be able to choose from a 74-Z Speeder Bike, Tie Advanced X1, T-65 X-Wing Starfighter and of course, the Millennium Falcon. The models feature an advanced 'reverse' propulsion system situated beneath the hulls, that allow them to accelerate to 25mph in just 3 seconds.
It also means that the craft are pushed into the air, rather than sucked, meaning the models can be made without holes in them – unlike some previous attempts - to further resemble the iconic craft. More exciting still, they are equipped with model specific coloured lasers so pilots with an eye for bullseyeing Womp rats can shoot each other out of the sky.
The blue sky thinking has been extended to the propellors too, which have been designed to look invisible to maintain the illusion of actually flying the 'real' thing. There will be stabilisation aids to help beginners but once you get the hang of the controls, you'll be able to perform flips and loops to evade any pursuers.
The packaging is pretty stunning too; each hand-painted model comes in a luxe box with a moulded relief of the spacecraft inside and it contains a display plinth (with light and sound effects), so your chosen craft will look equally stunning parked on a shelf.
Each will come individually numbered and the packaging wax sealed to please the die-hard collectors.
The one downside is that if you live in the US, getting hold of these might prove a little tricky. So far Propel only have the license for Europe and the Middle East; so if you're desperate to get your hands on one (or several), you'll have to find a retailer prepared to ship to America. Like us for instance. We'll update on availability as soon as possible.
These most definitely are the Star Wars toys you are looking for... Until then, check out our #StarWars merch here.
And this is the clip from the recent Star Wars Celebration London event, where the drones made their debut...


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款式: 40117616 颜色:
color is off
Adorable bandana was so excited to get it in the mail but like other FP products, the coloring is way off from the picture. I was expecting a more cool toned lighter purple with blue lettering but got a dark purple bandana with green lettering. Was kind of disappointed :( will be returning


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- "... You don't see the whole. The parts are crap, but you put it together you got a Firefly. Thing'll run forever they got a mechanic even half awake... Some people ain't lookin' for flash. She's a good catch."
- ―The scrapper boss
The Firefly-class is a series of Multipurpose, Mid-Bulk Transport boats designed by Allied Spacecraft Corporation. The hulls and engines are constructed at ASC's shipyard at New Burbank, Osiris, with final outfitting being outsourced to a dozen smaller companies, including the newly formed Firefly Ship Works on Hera.
All Fireflys feature a large rear engine module, wing-mounted engines capable of vertical takeoff and landing (VTOL), a centralized cargo section, and raised forward section holding the crew quarters and bridge.


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Pleasure Productions 2Favorites [+]
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Running Time: 56 minutes
Studio Name: LBO
Series: Pleasure Productions
Description: White hot 3-way action abounds in this second scorching video delight.
6 Scenes from Pleasure Productions 2
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Whether from a bustling city ward or tranquil suburban subdivision, a child's health and happiness depends less on where they're raised and more on who's raising them and how, experts say.
Fostering trusting and open relationships with children is the best possible way to protect them from the negative influences that exist in every community, says Katherine Tyson McCrea, professor of social work at Loyola University, director of the Institute for Advanced Research and Practice in the School of Social Work and editor-in-chief of Illinois Child Welfare.
"It's important for people to know that kids value love more than anything else, so for parents it's a good thing that's the case because that doesn't depend on the environment," she says
Similarly, children reared in close, trusting neighborhoods will be better off.
"Cohesive, involved neighbors are an important protection factor because they act like a safety net for the family," says Sonya Leathers, assistant professor in the Jane Addams College of Social Work at the University of Illinois at Chicago. In neighborhoods that lack unity, the neighborhood doesn't provide that net.
City and suburbs also share some drawbacks, such as crime.
"Clearly it depends on what part of the city you live in because the crime rate varies so much ... but frankly the suburbs can be quite dangerous if a child is driving with another who's been drinking. And suburban kids are just as capable of buying drugs or having parties with lethal results," Tyson McCrea says.
Similarly, lack of diversity is not merely a suburban issue.
In affluent areas of the city, children might be insulated from everyday city life and as time passes find it harder to cross cultures and relate to people from other backgrounds.
"The danger is that privilege is a barrier," Tyson-McCrea says.
Children who have more contact with different races and ethnicities will develop less stereotyped views and make more friends.
"This exposure will also help form their self concept, how they view themselves and the world," Leather says.
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Print advertisment created by Ogilvy, Uruguay for Ovacion, within the category: Media.
Read it, live it.
Creative Director: Vartan Chakiyian
Art Director: Gustavo Etchandy
Copywriter: Marindia
Highest Rated
When even the lay out of the web site looks better than the ad it self....
No one cares about child labor, eye donation or saving the Earth: NO PSA!!! http://adsoftheworld.com/forum/135094
Love this ad
Arrest the execution. It killed the idea!
Hate advertising? Make better ads.
here i found it
to AXAY555
Is not the same idea!
If you start the game of similar things in advertis you blow out all the ideas!.
Conclusion, i'm not agree at all, you can judge the idea and the execution if you want. But if you start with these kind of comments nothing on this website is really "autentic"
My opinion of the campaign, I like the idea and the artwork. Geometric layout.Have a clear intention. That's works for me.
Bien (y)
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|This is a disambiguation page. A number of articles are associated with the title Alice.
NOTE: If an internal link referred you to this page, you may wish to change the link to point directly to the intended article.
Alice may refer to:
- Alice's Adventures in Wonderland
- Alice Evans, actress (Eloise Hawking in 1977)
- Juliet's mother, who was named Alice in the casting call
- Alice, a background actress who played Alice and Fern


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The American Idol star shocked the audience with a routine which included kissing a male keyboard player and simulating oral sex with a male dancer.
The Parents Television Council denounced the show as "vulgar" and said it was "enough to alarm any reasonable parent".
PTC president Tim Winter said: "American teenagers - and especially teenaged girls - are literally under siege by the entertainment media.
"It is outrageous that children today cannot watch a televised awards programme for an industry that is built squarely on their backs. Teens comprise a huge portion of music sales, yet this is how they are treated? It is beyond contemptible."
The broadcast was "nothing short of tasteless and vulgar" and ABC buried their heads in the sand when Lambert promised to put on a "sexy" show, Mr Winter said.
ABC edited out the most shocking moments for its West Coast broadcast, but millions saw it live and the video has become a hit on YouTube.
After the performance, his first since American Idol, Lambert said: "I'm hoping people were entertained. For those who weren't, maybe I'm not your cup of tea."
He also accused the media of double standards, saying: "Women performers have been pushing the envelope sexaully for the past 20 years and all of a sudden a male does it and everybody goes, 'Oh, we can't show that on TV'. To me that's a form of discrimination and it's a double standard, and that's too bad."


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Our purpose is to enable everyone to do the work they love. By sharing the journey that we're on to understand ourselves and realise our purpose, we hope to inspire others in the AI sphere to be proactive in considering their relationship with society.
Why AI and why now?
Satalia was birthed during a period of relative calm for the Artificial Intelligence (AI) ecosystem. We've seen the hype grow exponentially since. Though there have been successive peaks and troughs in the expectations for AI over the last half century, there really seems to be a sense that 'this time, this is it!'
With an unprecedented amount of investment pouring into the field, many leading thinkers have turned their attention to the societal impact of these new technologies.
Authors including Nick Bostrom and James Baratt have set out fairly bleak pictures of what they expect will happen should the human race build machines with the general intelligence of humans before the right safeguards are in place. Not to mention the joint statement from 8,674 to date, including some of the best known public figures from science and industry, amongst them Elon Musk and Stephen Hawking.
Estimates of when we'll achieve this level of artificial intelligence vary greatly from a few years to never. But if we carry on along our current trajectory there can be no doubt that tectonic shifts in the socio-industrial landscape will occur. The world of work will change beyond recognition for most people and new forms of industry will be born. And who knows about politics and religion?
I, for one, welcome our new robot overlords…
The introduction of these algorithmic technologies has already produced mild versions of these shifts, ones that we've probably all observed in our day to day lives. The effects usually cut both ways. There are winners and losers. But that's life, isn't it?
Let's face it, Uber has made getting from A to B a lot easier for an enormous amount of people. Unless you're a hard-core trade unionist you'll probably accept that the net impact has been positive.
However it's also true that taxi drivers have had a pretty rough ride (Author's note: there will be no apologies for mediocre puns). The pushback has in some cases literally brought cities to a standstill. This is the sort of societal upheaval that AI technologies can provoke.
Now imagine if Uber-scale disruption happened across all major industries. At the same time. Finding it hard to picture? Us too. It seems such an unfamiliar, faraway concept, but it's not improbable.
"…imagine if Uber-scale disruption happened across all major industries. At the same time."
For AI companies —using the term loosely to mean any organisation using machine learning or optimisation in their products, services, or operations — there is a very real imperative to find some way of navigating the social quagmire of disruptive change.
For people not familiar with the mechanics or capabilities of AI technologies the headlines are fairly terrifying:
Artificial Intelligence could kill us all.
You Should Be Afraid of Artificial Intelligence
Steve Wozniak: The Future of AI Is 'Scary and Very Bad for People'
Stephen Hawking: Artificial intelligence could wipe out humanity
Not to mention that picture of the Terminator that's always used in the media. It's sort of ironic since we have much more to fear from super-intelligent software than from humanoid robots.
Nonetheless, if you're using AI and your goal is to change the world then you'd better make damned sure people understand why.
"…if you're using AI and your goal is to change the world then you'd better make damned sure people understand…
So what is organisational purpose?
The concept of organisational purpose has been floating around for a few years now. Expert practitioners in the field are helping companies to attract top talent and drive innovation. On the whole though, most of us mortals haven't a clue what purpose is, how to establish your organisation's purpose, or what to do with one once you've got one!
Fear not, you are not alone! We started exploring organisational purpose in earnest perhaps only twelve months ago. Our understanding of the theory and its applications is far from complete. Nevertheless we thought that by sharing some of our learning and experiences we might open the door for others to find their purpose too.
Purpose is not usually a mission or a vision (though it might be close), and it's not quite corporate responsibility either. Those deal with the what and how of your business. Purpose is your organisation's why. Why does your organisation exist? Why are your people working together? Why do they make the decisions that they do?
"Purpose is your organisation's why."
Simon Sinek nicely packages up the concept of why into 'golden circles':
Purpose consultancy Within People have put together an excellent slide deck giving guidance on understanding and defining your organisation's purpose:
What's Satalia's purpose?
To some extent we're still figuring it out. We've been through several successive waves of thought and have most recently settled on the notion that we exist to enable everyone to do the work they love. This works in two ways. Inside and outside.
"…we exist to enable everyone to do the work they love"
Enabling our people to do the work that they love
Looking inward we tried to understand what binds our merry band of technologists, academics and entrepreneurs together as a company. The word company derives from a group pursuing the same purpose, after all. We came up with all sorts of clever and noble sounding reasons, but after a few weeks of reflection each one fell apart as simply wishful thinking.
The breakthrough came when we swept aside our desires to be good people and just focused on our selfish motivations. This might seem counterintuitive. It felt like it! But what we discovered is that we're attracted to work at Satalia because we have the freedom to do what we want.
We've spent countless hours researching, designing, testing and implementing non-traditional organisational structures to make this feasible. It's been extremely challenging at times but the self-managing structure that we've adopted will allow us to grow the company whilst retaining our freedom.
Self-determination is a key factor in motivation and satisfaction at work, and we're by no means the only company trying to solve the problem of enabling it at scale. But freedom to choose what you want to do is no good if you're out of a job.
"…we exist to enable everyone to do the work they love"
Enabling everyone to do the work that they love
As the sections above might suggest, we're acutely aware of the potential for our technology to displace people from their jobs or take over key activities.
To this some people say "Great! I hate that part of my job. Let a computer do it and I'll have more time to deal with other things." Others who are specialised in solving particular types of complex problem — people typically in high demand — are mortified by the idea of a computer doing their job.
In the short term we're concerned with the immediate social impact of companies adopting our tech. Whose jobs will be at risk or augmented? How large will the impact be?
As a starter for ten, try the Nesta "Will a robot take my job?" quiz .
The work you love today
Companies might be interested in optimisation because of cost savings. Fair enough. But if that means laying off a team of people who loved their jobs and will find it difficult to find work elsewhere, that's not cool.
We're not in the business of replacing humans with computers. We're in the business of designing and building high performance tools. So we ask ourselves questions like:
- To what extent does it make sense to remove human decision making from the problem we're trying to solve with optimisation?
- What is this client's attitude toward taking care of its people?
- How can we raise our clients' awareness of the social impact of our technology?
- How can we help our clients to plan for redeployment of those affected?
- How can we increase, rather than limit, the options available to those impacted?
- Can we train employees whose value to their company is at risk to be expert operators of the new optimisation tools?
"We're not in the business of replacing humans with computers."
To answer these questions and achieve positive outcomes for people, we need to deal with more than one part of our clients' businesses. We need to inspire senior leadership and engage with HR (or People) functions. As well as those on the front line who will bear the brunt of the impact if we fail to live up to our purpose.
Looking further into the future the imperative is even greater. As optimisation and other AI technologies become ubiquitous in society we may find that there aren't many jobs left for humans to do at all.
Work that you'll love in the future
Whether people hate their work or have found their true calling, human beings have an inherent need to contribute to their social group in some way. It gives us a sense of meaning, a sense of purpose.
Even the most seemingly mundane of jobs can do the trick. We've seen this in studies on the elderly and their mental health. Who are we to take this away for the sake of efficiency?
So, we're confronted with a difficult dynamic. We are going to have to think very deeply about how we value and reward people's contributions to society when many types of labour that were previously high in value become commoditised.
"We are going to have to think very deeply about how we value and reward people's contributions to society…"
Why is it so hard to earn a living as an artist or musician? Why are teaching and nursing some of the most personally rewarding professions, but also some of the lowest paid. Does it always have to be so? Can't we have both?
The opportunity is to use AI technology to free people from drudgery. But the risk is that we don't think carefully enough about the nature of work, or create the conditions in society to shift the current paradigm.
To harness the full positive potential of optimisation and AI, we need to enable people to do the work they love, both now and in the future.
What's next?
Whilst we've come on strides in our understanding we're still a long way from embedding our purpose into all of our activities. Our ambition is to integrate this thinking into each of our business functions. To have it woven into our cultural fabric at the most fundamental level.
"What's important is that all of us in the artificial intelligence ecosystem take action."
Certainly, our thinking will evolve as we learn new things and the world changes around us. What's important is that all of us in the artificial intelligence ecosystem take action. Action to prevent the wonderful technologies that we're creating from doing harm. Action to make sure that we create fair and universally positive outcomes. And action to understand ourselves before we are sailing in ships that are pulled by currents beyond our control.
Drop us a line to talk problems, purpose or possibilities any time.
Love from Team Satalia