text:
Join Now! It's FREE. Get full access and benefit from this site
Before beginning, you may want to use the MD5 sum provided at the VM download page to verify your download. On Mac OS X and *nix systems, you can use the command
md5 learning_puppet_vm.zip and compare the output to the text contents of the
learning_puppet_vm.zip.md5 file provided on the download page. On Windows systems, you will need to download and use a tool such as the Microsoft File Checksum Integrity Verifier.
Get an up-to-date version of your virtualization software. We suggest using either VirtualBox or a VMware application appropriate for your platform. VirtualBox is free and available for Linux, OS X, and Windows. VMware has several desktop virtualization applications, including VMWare Fusion for Mac and VMware Workstation for Windows.
The Learning VM's Open Virtualization Archive format must be imported rather than opened directly. Launch your virtualization software and find an option for Import or Import Appliance. (This will usually be in a File menu. If you cannot locate an Import option, please refer to your virtualization software's documentation.)
Before starting the VM for the first time, you will need to adjust its settings. We recommend allocating 4GB of memory for the best performance. If you don't have enough memory on your host machine, you may leave the allocation at 3GB or lower it to 2GB, though you may encounter stability and performance issues. Set the Network Adapter to Bridged. Use an Autodetect setting if available, or accept the default Network Adapter name. (If you started the VM before making these changes, you may need to restart the VM before the settings will be applied correctly.) If you are unable to use a bridged network, we suggest using the port-forwarding instructions provided in the troubleshooting guide.
Start the VM. When it is started, make a note of the IP address and password displayed on the splash page. Rather than logging in directly, we highly recommend using SSH. On OS X, you can use the default Terminal application or a third-party application like iTerm. For Windows, we suggest the free SSH client PuTTY. Connect to the Learning VM with the login
root and password you noted from the splash page. (e.g.
ssh root@<IPADDRESS>) Be aware that it might take several minutes for the services in the PE stack to fully start after the VM boots. Once you're connected to the VM, we suggest updating the clock with
ntpdate pool.ntp.org.
You can access this Quest Guide via a webserver running on the Learning VM itself. Open a web broswer on your host and enter the Learning VM's IP address in the address bar. (Be sure to use
http://<ADDRESS> for the Quest Guide, as
https://<ADDRESS> will take you to the PE console.
For issues with Puppet Enterprise that are not specific to the Learning VM, see the Puppet Enterprise Known Issues page.
The Learning VM version 2.29 has an error in the instructions for this quest. The cowsay package declaration should include
provider => 'gem', rather than
ensure => 'gem'.
If you continue to get puppet run failures related to the gem, you can install the cached version manually:
gem install /var/cache/rubygems/gems/cowsay-0.2.0.gem
The quest tool uses a series of Serverspec tests for each quest to track task progress. Certain tasks simply check your bash history for an entered command. In some cases, the
/root/.bash_history won't be properly initialized, causing these tests to fail. Exiting the VM and logging in again will fix this issue.
It is also possible that we have written the test for a task in a way that is too restrictive and doesn't correctly capture a valid syntactical variation in your Puppet code or another relevant file. You can check the specific matchers by looking at a quest's spec file in the
The Learning VM's Quest Guide is accessible at
http://<VM's IP Address>. Note that this is
http and not
https which is reserved for the PE console. The PE console will prompt you for a password, while no password is required for the Quest Guide. (The Quest Guide includes a password for the PE console in the Power of Puppet quest: admin/puppetlabs)
The password to log in to the VM is generated randomly and will be displayed on the splash page displayed on the terminal of your virtualization software when you start the VM.
If you are already logged in via your virtualization software's terminal, you can use the following command to view the password:
cat /var/local/password.
Possibly, but we don't currently have the resources to test or support the Learning VM on these platforms.
It may take some time after the VM is started before all the Puppet services are fully started. If you recently started or restarted the VM, please wait a few minutes and try to access the console or trigger your puppet run again.
Also, because the Learning VM's puppet services are configured to run in an environment with restricted resources, they are more prone to crashes than a default installation with dedicated resources.
You can check the status of puppet services with the following command:
systemctl --all | grep pe-
If you notice any stopped puppet-related services (e.g. pe-puppetdb), double check that you have sufficient memory allocated to the VM and available on your host before you try starting them (e.g.
service pe-puppetdb start).
If you get an error along the lines of
Error 400 on SERVER: Unknown function union... it is likely because the
puppetlabs-stdlib module has not been installed. This module is a dependency for many modules, and provides a set of common functions. If you are running the Learning VM offline, you cannot rely on the Puppet Forge's dependency resolution. We have this module and all other modules required for the Learning VM cached, with instructions to install them in the Power of Puppet quest. If that installation fails, you may try adding the
--force flag after the
--ignore-dependencies flag.
First, ensure that you have an up-to-date version of your virtualization software installed. Note that the "check for updates" feature of VirtualBox may not always work as expected, so check the website for the most recent version.
If your network connection has changed since you loaded the VM, it's possible that your IP address is different from that displayed on the Learning VM splash screen. Log in to the VM via the virtualization directly (rather than SSH) and use the
facter ipaddress command the check the current address.
Some network configurations may still prevent you from accessing the Learning VM. If this is the case, you can still access the Learning VM by configuring port forwarding.
Change your VM's network adapter to NAT, and configure port forwarding as follows:
Name - Protocol - HostIP - HostPort - GuestIP - GuestPort
Once you have set up port forwarding, you can use those ports to access the VM via ssh (
ssh -p 2222 root@localhost) and access the Quest Guide and PE console by entering
http://localhost:8080 and
https://localhost:8443 in your browser address bar.
The Learning VM uses a tool called tmux to allow us to display the quest status. You can scroll in tmux by first hitting control-b, then [ (left bracket). You will then be able to use the arrow keys to scroll. Press q to exit scrolling.
Reduce the VM's processors to 1 and disable the "I/O APIC" option in the system section of the settings menu.
15-05-2017 Hits:491 SVN(Subversion) Mantosh
The old way of software development where the release engineers ran from one mates to another mates to keep track on Developers to know which module they are working and...Read more
12-05-2017 Hits:552 Continuous Integration Mantosh
Today we are going to discuss about Cloud based continuous integration tools. As we already discussed about Continuous integration, it's benefits and top continuous integration tools in our previous article...Read more
02-05-2017 Hits:560 Build Management Mantosh
These days in software industry the process of software development very much rely upon best practices of various tools. The software development teams use various tools like project management, release...Read more
27-04-2017 Hits:809 Scripting Mantosh
This is the time of DevOps in software industry and DevOps uses different different languages for deployment automation and for software development. This is the reason if you are a...Read more
25-04-2017 Hits:638 Git Mantosh
This is the era of DevOps where automation and collaboration is the key of success in software industry. This is the reason code availability is for everyone in the team...Read more
24-04-2017 Hits:485 General Mantosh
Cloud computing is one of the trends which is going in IT industry these days. The traditional way of building IT environment is now shifting towards the cloud computing. This...Read more
21-04-2017 Hits:337 Unix - Linux Rajesh Kumar
Tasksel – Easily/Quickly Install Group Softwares in Debian and Ubuntu If you started out using an RPM-based distro before advancements like "yum" or "apt-rpm", you're loving the magic of "apt-get" on...Read more
20-04-2017 Hits:641 General Mantosh
This is the age of Internet and social media and people's spend more and more time on to the net and social networks. And when people spend their time somewhere...Read more
20-04-2017 Hits:398 IT Infrastructure Monitoring Tools Rajesh Kumar
Starting Nagios Core Init Script: The easiest way to start the Nagios Core daemon is by using the init script like so: /etc/rc.d/init.d/nagios start Manually: You can start the Nagios daemon manually with...Read more
20-04-2017 Hits:566 IT Infrastructure Monitoring Tools Rajesh Kumar
How to start and stop Naxios XI Server? How to stop Naxios XI Server? service nagiosxi stop service npcd stop service ndo2db stop service nagios stop service postgresql stop service mysqld stop service httpd stop How to stop Naxios...Read more
20-04-2017 Hits:302 IT Infrastructure Monitoring Tools Rajesh Kumar
Step by step procedures to Install Nagios agent in Linux > cd /tmp > mkdir software > cd software > yum install wget > wget https://assets.nagios.com/downloads/nagiosxi/agents/linux-nrpe-agent.tar.gz > tar -zxvf linux-nrpe-agent.tar.gz > cd linux-nrpe-agent > ./fullinstallRead more
20-04-2017 Hits:580 IT Infrastructure Monitoring Tools Rajesh Kumar
Step by step proceduress to Install Nagios XI Server in Centos 7 #Update your centos os > yum install update > yum install wget # to install network utility e.g. ping. (Optional) > yum install...Read more
20-04-2017 Hits:359 General roushan
DevOps is merging the path between Developers and Operations teams. It's all about the agility and automation. Servers, in DevOps easily can be replaced instead of keeping it and nurture...Read more
19-04-2017 Hits:606 General Mantosh
Few days ago, I published an article in which i covered the topic Top five release management tools which are high in trend these days. Today, I am going to...Read more
19-04-2017 Hits:1164 Interview Questions & Answers Rajesh Kumar
Q. What is GIT? GIT is a distributed version control system and source code management (SCM) system with an emphasis to handle small and large projects with speed and efficiency. Q. What...Read more
18-04-2017 Hits:507 Incident and Bug Tracking Systems Mantosh
Just imagine a situation when you put so much efforts to build a software or application and release it and after that users get error or issues in it on...Read more
18-04-2017 Hits:656 General roushan
First of all, we should know what Operations Team does. So, I will start with responsibilities of operations team and will move forward to see DevOps role in Operations at...Read more
16-04-2017 Hits:509 IT Infrastructure Monitoring Tools Mantosh
There are various types of tools used by DevOps professionals in their work environment from Continuous integration tools to Virtualization to configuration Management to monitoring and the list goes on...Read more


text:
Walter is a Managing Partner. He shares responsibility for management of the firm and focuses on investments in the business services, food and consumer sectors.
He is a director of Salient CRGT, Sigma Corporation, Spice Chain Corporation, Wenner Bread Products and Whitebridge Pet Products. Walter's prior investments and directorships include Alfalfa's, Aspire Media, F+W Media, H-E Parts, Health Valley Foods, Mercer Foods LLC and SI International.
Walter joined Frontenac in 1994 from Bear, Stearns & Co. Inc, where he was an Analyst in the investment banking division.
A Pittsburgh native, Walter graduated cum laude from Dartmouth College and received an MBA with distinction from the J.L. Kellogg School of Management at Northwestern University.
One South Wacker
Suite 2980
Chicago, IL 60606
Tel: 312.368.0044
About Frontenac
Our Team
Contact Us
We invest in high quality companies, primarily in the food, industrial and services industries. We work with business owners, almost always families, founders or entrepreneurs, as they address complex transition issues of liquidity, management enhancement and growth planning.
Copyright © 2015 Frontenac Company. All rights reserved.

subset: commoncrawl


text:
powerfull video editing program ,you can apply varity and wide range of effects
Freeware is computer software that is available for use at no cost or for an optional fee.
Tutorial not found. Let know us about any useful video tutorial.
cut, split, disply, free, video editting, video cutter, video splitter, frame capture, movie cutter, movie splitter, image, pictures, video decompiler, video capture, video converter, avi, mpeg, wmv, asf, and mov
Minor Update


text:
Telephone & Addresses
(1 - 4 from 63
Jimmy Johnson, Cape Girardeau, US, W Mount Dr
View Jimmy's social profiles and photos on Facebook, MySpace, and +40 Networks.
Jimmy Johnson, Carson, US, Jot Em Down Rd
View Jimmy's social profiles and photos on Facebook, MySpace, and +40 Networks.
Jimmy Johnson, 49, Chesapeake, US, Mill Bridge Way, Unit WA
View Jimmy's social profiles and photos on Facebook, MySpace, and +40 Networks.
Jimmy Johnson, 47, …go, US, S Springfield Ave
View Jimmy's social profiles and photos on Facebook, MySpace, and +40 Networks.
Yellow Page contacts
Network Profiles
(1 - 4 from 506
MySpace Profile: Jimmy Johnson (whoisjimjones)
Fair Lawn, NJ
(1 - 4 from 60
Emergency Planning, Frying Pan to Fire
07.08.2012 [Patch.com] - After the exercise, Fire Marshal Jimmy Johnson suggested to Herald that the fire marshal's office be included in such an evacuation, because the firefighters would have their hands full helping people evacuate. A fire marshal on scene would be able to
'Jimmy Johnson: A Football Life' - NFL Videos - NFL.com
▶Here's the trailer for next week's "A Football Life," featuring former head coach Jimmy Johnson.
It's been 5 years since Jimmy Johnson sucked on 'Survivor' - SBNation ...
It's been five years since Jimmy Johnson went on Survivor: Nicaragua. He was eliminated after eight days. It's time to acknowledge just how ...
Dak or Romo? Ex-Cowboys coach Jimmy Johnson has the answer ...
Jimmy Johnson doesn't coach the Cowboys anymore, but if he did, there wouldn't be any controversy at the quarterback position when Tony ...
Business Profiles
(1 - 4 from 8
Xing: Jimmy Johnson
Leiter / Dortmund / bau und co
Xing: Jimmy Johnson
owner / Plano, Vereinigte Staaten
Xing: Jimmy Johnson
CEO / Denver / Sports
Researchgate: Jimmy Johnson
Columbus, OH, United States
Private Homepages
(1 - 4 from 9
History – Jimmy Johnson Music
Jimmy Johnson's first appearance as a paid guitarist was at the age of 15 at a Saturday night dance at the National Guard Armory in Tuscumbia, Alabama.
Law Offices of Jimmy Johnson PLLC - Home
How Can We Help You?Whether you're individual immigrantion applicants, solo or large law firms, or non-profit organizations that represent individuals or alien ...
Jimmy Johnson Music - An Original Swamper
Jimmy Johnson Auto Sales
Used Cars Dyersburg At Jimmy Johnson Auto Sales ,our customers can count on quality used cars, great prices, and a knowledgeable sales staff.
NCDEQ - APNEP- Staff
Jimmy Johnson Coastal Habitats Coordinator email@example.com 252-948-3952. APNEP, 943 Washington Square Mall • Washington, NC 27889.
(1 - 4 from 77
ratemyteachers: Jimmy Johnson
Bronx, NY, M.s.118
ratemyteachers: Jimmy Johnson
Bronx, NY, William W. Niles Middle School
ratemyteachers: Jimmy Johnson
Enid, OK, Chisholm High School
ratemyteachers: Jimmy Johnson
Galena Park, TX, North Shore Sr High School
Celebrities & Politicians
(1 - 2 from 48
Bad news
(1 - 4 from 51
legacy.com: Jimmy Johnson
Jimmy Johnson Macon, GA- Memorial services for Jimmy Johnson will be 3 PM Friday, November 18, 2016 at Memorial Baptist Church 697 Norris St., Macon. Rev. James O. Miller will
legacy.com: Jimmy Johnson
Jimmy Johnson, 78, of Sanostee, passed from this life Thursday, March 10, 2016, in Bloomfield. He was born March 27, 1937, in Sanostee. Graveside services and interment will be at
findagrave: Johnson, Jimmy
08/21/1816 - 04/2/1900, Steelville (Crawford County, Missouri)
Minnie K. Puckett
03.08.2012 [Staunton News Leader] - ... Teresa Anson, Susan Wilcher, Jimmy Johnson, Kristie Johnson, Robbie Montgomery, Mark Montgomery, Crystal Thomas, Cathy Humphreys and Dana Metz; 19 great-grandchildren; eight great-great grandchildren and caregivers, Donnie and Evelyn Byram
Books & Literature
Jimmy Johnson (Author of Turning the Thing Around)
Jimmy Johnson is the author of Turning the Thing Around (3.93 avg rating, 45 ratings, 4 reviews, published 1993), Beaucoup Arlo & Janis (4.25 avg rating,...
Jimmy Johnson - Keynote Speakers | LAI
[Book author and NFL star Jimmy Johnson for your next event. Call 1-800-SPEAKER or see more here.]
Inside the Whimsy Works: My Life with Walt Disney Productions
In this never-before-published memoir from the vaults of the Walt Disney Archives, Disney Legend Jimmy Johnson (1917-1976) takes you from his beginnings ...
Jimmy Johnson Quotes - BrainyQuote
Enjoy the best Jimmy Johnson Quotes at BrainyQuote. Quotations by Jimmy Johnson, American Coach, Born July 16, 1943. Share with your friends.
(1 - 4 from 13
Jimmy Johnson Songtexte, Lyrics & Übersetzungen
Songtexte von Jimmy Johnson mit deutschen Übersetzungen, Lyrics, Liedtexte und Musik-Videos kostenlos auf Songtexte.com
Jimmy Johnson Concert Setlists | setlist.fm
Get Jimmy Johnson setlists - view them, share them, discuss them with other Jimmy Johnson fans for free on setlist.fm!
Jimmy Johnson" Lyrics by Lagwagon: I remember well, holding him down Silent under due his torture, tortures me now Pounding the mold, forth...
Related Documents
Edmunds gets high marks, bonus
01.08.2012 [Tampa Bay Newspapers] - Three councilors – Waters, Jim Quinn and Mayor Jimmy Johnson – gave him a perfect score. John Counts handed out a 71, Thomas Barnhorn delivered a 70.5 and Bob Matthews a 70. "While other cities have needed to increase their millage rates, we have
Comics I Don't Understand » Jimmy Johnson says that what Janis ...
14.04.2012 15 Responses to "Jimmy Johnson says that what Janis actually asked for is irrelvent to the gag". Arthur Apr 13th 2012 at 02:19 am 1. The joke is that voice recognition is not perfect and can lead to humorous possibilities. In ...
Jimmy Johnson Videos | Photobucket
Browse Jimmy Johnson pictures, photos, images, GIFs, and videos on Photobucket
Jimmy Johnson Live Review
Blues On Stage. Comprehensive guide to blues in the Twin Cities. Blues from around the world! One of the largest live review sections on the net.
Video & Audio
(1 - 4 from 34
BlinkX Video: NASCAR's Jimmy Johnson schools Hoda and Kathy Lee on driving
Dec. 18: NASCAR's Jimmy Johnson schools Hoda and Kathy Lee on racecar driving. (Today Show) NASCAR - Jimmie Johnson - autoracing - Motorsports - Sports - 01.18.2010, MSNBC
BlinkX Video: Jimmy Johnson Inducted Into 2012 CFB HOF
NFL Writer, Clifton Brown weighs in on Jimmy Johnson being inducted in to the college football hall of fame, the success Al Golden has had at Miami, and a four-team playoff... - 05.17.2012, TotalCollegeSports
BlinkX Video: Coach Johnson: 'Survivor' saved my life
Former NFL coach Jimmy Johnson tells CNN's Rick Sanchez how a reality show gave him a wake up call. - 09.24.2010, CNN
Jimmy Johnson Extenze Commercial.mpg - YouTube
Jimmy Johnson Extenze Commercial
(1 - 4 from 128
Gosselin: Rookie QBs are expected to struggle, but don't tell RG3
08.08.2012 [Dallas Morning News (subscription)] - Sitting at the hotel bar with Jerry Jones that night in Orlando, Fla., in March 1994 when he decided he'd had enough of Jimmy Johnson as coach of the Cowboys. SOMETHING PEOPLE DON'T KNOW ABOUT ME: I played hockey for a media all-star team in
Wilford Ray Jennings
08.08.2012 [Mountain Statesman] - Thursday, August 9, 2012 at 11:00 a.m. Interment will follow at Woodsdale Memorial Park, with Pastor Jimmy Johnson officiating. In lieu of flowers, memorial donations can be made to Amedisys Hospice of Anmoore, 67 Casino Drive #102, Anmoore, WV
Stewart tackles local tracks
08.08.2012 [Lebanon Daily News] - The Junior SlingShots saw Paul Ennes grab the win by beating Wes Hearn, Dale Kober, Jimmy Johnson and Kevin Oliver. Kutztown Fair Speedway. Last Wednesday at Kutztown Fair Speedway in the Pa. Dutch Classic All-Star SlingShot 30-lap feature event
Gosselin: Cowboys had one of the best talent evaluators I've seen
06.08.2012 [Dallas Morning News (subscription)] - But Jimmy Johnson was the best I'd ever seen at it. The Cowboys won three Super Bowls with players Johnson hand-picked. When Johnson stopped selecting players in the spring, the Cowboys stopped winning championships in the fall. Q: How serious do
Reports & Statements
(1 - 4 from 452
Twitter Posts: Lucas Carreras (maldini3fan)
2012.07.13 RT @FisolaNYDN: Jimmy Johnson and Barry Switzer look like saints compared to JoePa now
NASCAR Chase continues with Jimmy Johnson surging (Q&A with ...
Jimmy Johnson punched his ticket into the Championship Four with his win last Sunday in Martinsville as he took the lead with less than 100 ...
Jimmie Johnson on triathlons, 48, his beard
01.08.2012 [ESPN (blog)] - Do you still get confused with the other Jimmy Johnson, the famous former football coach? "Not too much. Forever, people have been spelling my name with a 'y.' I saw Jimmy not too long ago and he said people were now spelling his name with an 'ie.' I
Watters, now suing NFL, was a gamer
24.07.2012 [ESPN (blog)] - "Watters stayed on his knees, clinging to tight end Jimmy Johnson. After staying on the field briefly, Watters was helped to the sideline, his legs wobbling noticeably." Watters, who began his career with the San Francisco 49ers and ended it with the
(1 - 20 from 236
Las sesiones del campamento de entrenamiento ya no son tan ...
31.07.2012 [ESPN Deportes] - "Jimmy [Johnson, ex entrenador en jefe de los Cowboys] era durísimo y mejor ni quejarse porque no durabas ahí un par de días más", agregó. Los equipos de la NFL, según el nuevo contrato colectivo de trabajo firmado el año pasado, no pueden sostener
Forever feuding Jerry Jones, Jimmy Johnson still arguing over ...
It's been 20 years since Cowboys owner Jerry Jones and former Dallas coach Jimmy Johnson dramatically parted ways after two straight Super ...
Browns' brass understands that ownership switch could bring ...
01.08.2012 [Yahoo! Sports (blog)] - Sometimes, that's for the best, as it was when Jerry Jones sent the great Tom Landry packing in favor of his old Arkansas teammate Jimmy Johnson. The malaise brought about by Landry's declining years as a coach was soon replaced by a dynasty team that
Cowboys: Jerry Jones, Haunted by Jimmy Johnson
The Dallas Cowboys owner and general manager Jerry Jones wants to show the world he can win without former head coach Jimmy Johnson.
Browns' brass understands that ownership switch could bring ...
01.08.2012 [Yahoo! Canada Sports (blog)] - Sometimes, that's for the best, as it was when Jerry Jones sent the great Tom Landry packing in favor of his old Arkansas teammate Jimmy Johnson. The malaise brought about by Landry's declining years as a coach was soon replaced by a dynasty team that
FLW Fishing: JIMMY JOHNSON - Angler Profile
JIMMY JOHNSON - Profile, Latest News, Photos, Videos, Tournament Statistics.
Rookies making early impact in Cleveland camp
30.07.2012 [Yahoo! Sports] - Is that from a copy of Jimmy Johnson's retirement press release from the early '90s? Last I heard, teams play their games on the field, not from the living room lounger. How 'bout them COWBOYS?! Stevendtoo 7 days ago. The Cowboys won't win any playoff
Iconic Cowboys coach Jimmy Johnson: There's a place for Gators ...
Iconic Cowboys coach Jimmy Johnson: There's a place for Gators legend Tim Tebow in NFL.
JIMMY JOHNSON | HYPEBEAST - Hypetrak
JIMMY JOHNSON - The leading online destination for men's contemporary fashion and streetwear. Shop at our store and also enjoy the best in daily editorial ...
Jerry Jones Admits He Handled Jimmy Johnson Situation Wrong | 12up
After winning a national championship at the University of Miami, Jerry Jones hired Jimmy Johnson to be the Cowboys head coach prior to the ...
Jimmy Johnson - At-Large - Libertarian Party of Nevada
Jimmy Johnson. At-Large Representative of the Libertarian Party of Nevada. facebook http://www.facebook.com/jimmy.johnson.397501.
Jimmy Johnson - REMAX Kauai
Jimmy Johnson. (R) Realtor Associate. 4-831 Kuhio Hwy Suite #428 Kapa'a, HI 96746 United States. Email This Agent. My Realtor Web Site. Office Phone
Jimmy Johnson - University of Miami Sports Hall of Fame - UM Sports ...
Head Coach Jimmy Johnson was the cornerstone of the Hurricane football program that personified the team on the 1980's. After taking over the helm of the ...
Jimmy Johnson Is the New Colonel Sanders in Latest KFC Ad - Video ...
Jimmy Johnson is the Latest to Don the White Suit in New FOX NFL Sunday Tie-In.
Jimmy Johnson on Chip Kelly's roster makeover in Philadelphia | The ...
It's not unlike what another college coach once did with an NFC East power—and Jimmy Johnson built a winner in the 1990s. Johnson weighs ...
Jimmy Johnson on son's Tranquil Shores rehab facility | WTSP.com
Former NFL head coach Jimmy Johnson opens up about his son Chad's Tranquil Shores drug and alcohol rehabilitation facility in Madeira ...
Jimmy Johnson | PigeonsandPlanes
Stay tuned to PigeonsandPlanes for new Jimmy Johnson content daily.
Jimmy Johnson | WME|IMG Speakers
Jimmy Johnson, the architect of the Dallas Cowboys' consecutive victories in Super Bowls XXVII and XXVIII, former Miami Dolphins coach, and current member ...
Jimmy Johnson, Agent in NYC - Compass
Jimmy Johnson is a Licensed Real Estate Salesperson | Licensed as James P. Johnson for Compass in NYC. View Jimmy's recent transactions and get in touch ...
Jimmy Johnson, Electric Bass/MD : James Taylor
Jimmy Johnson, Electric Bass/MD. JimmyJohnson1. WHEN AND WHERE WAS YOUR FIRST GIG? I joined the musician's union when I was 13 ...
sorted by relevance / date


text:
TECHNOLOGY: Gadgets often are provided; so go easy
Today's college students rely on laptop computers and other gadgets as much as their trusty No. 2 pencils. But smart shopping can keep high tech from being high cost.
Easiest way to save: Call your college first. Some schools include computers or other devices in the price of tuition. Wake Forest University and Seton Hall University give students laptops, for example. Duke University this year will give freshmen digital music players. Other schools may offer discounts.
Other colleges ask students to buy certain products. West Virginia Wesleyan College recommends students buy Dell laptops. Students in Washington State University's graduate business school are required to buy laptops equipped for wireless Internet.
Once you know what's provided or required, you can shop for two tech essentials:
•Laptop computers. Most students prefer to have their own computers. Desktop PCs are OK, but laptops can be carried to class, the library or the local coffeehouse. Wireless Internet capability is a plus because many colleges have wireless networks, says Jean Boland, technology head at Morrisville State University in New York.
Don't forget accessories. A carrying case protects a laptop. A printer saves trips to the copy shop. Frequent users may want an external monitor, mouse and keyboard. A full setup can easily cost $2,000, says NPD Group PC analyst Stephen Baker. IBM, Dell, Gateway and others are offering back-to-school deals.
•Cell phones. So many students carry cell phones that schools such as Winona State University in Minnesota and the University of Denver no longer provide long-distance service in dorms. Many students prefer cell phones because they don't have to split the phone bill with roommates. Students far from home can buy unlimited long-distance cell phone plans. Friends and family plans also can cut costs.
But before buying a phone, make sure the cellular carrier has service near your school. Look for plans that can be adjusted once you arrive at college, and know how many minutes you need. Phones are often free with a cellular plan, Baker says. Plans vary from less than $20 to more than $100 a month.
Students with big budgets may want other tech gadgets. Music fans can tote tunes with MP3 players, which generally cost $100 to $300. Digital cameras usually cost $150 to $500. Busy students may want a personal digital assistant (PDA) to keep schedules and phone books organized. They're usually $100 to $400.
But academic experts say students shouldn't break their budgets on technology. If a class requires a specialized piece of equipment, the school will almost always provide it.
"You don't need a big fancy computer, just a basic tool," says Joe Whetstone, vice president for information technology at Winona State.
As for other gadgets, ask yourself if you really need it, he says.
By Michelle Kessler
INSURANCE: Homeowner policies may need a booster shot to cover laptops, etc.
Years ago, college students could stuff all their worldly possessions in a duffle bag. Today, many arrive on campus with laptops, stereo equipment and televisions worth thousands of dollars.
If you're the parent of a college-bound student, talk with your insurance agent to find out what your homeowner's insurance covers, says Jeanne Salvatore, vice president of consumer affairs for the Insurance Information Institute. Some policies limit coverage of items stored away from your home to 10% of total coverage. If your policy covers contents worth $25,000, your child's coverage will be limited to $2,500 — barely enough to cover a stolen laptop.
Also, pay close attention to your policy's limits and restrictions, says Donald Strejeck, president of Safeware, which provides separate insurance for personal computers. Some homeowner policies don't cover accidental damage to computers, so if your child drops her laptop or spills beer on the keyboard, you may be out of luck.
If your child is taking a particularly valuable item to campus, such as jewelry or an expensive camera, you may need to add a "floater" to your policy. Floaters cover specific items, usually at full replacement cost.
Finally, while your policy probably will cover the contents of your child's dorm room, it may not extend to off-campus housing, says Madelyn Flannagan, vice president of education for the Independent Insurance Agents & Brokers of America. If your child plans to live in an apartment or group house, you may need to buy renter's insurance.
Sending a child to college also could affect car insurance. If your child leaves his car at home and attends school at least 100 miles away, you may qualify for a substantial reduction in premiums, Flannagan says.
If your child takes a car to school, report the new location to your insurer. If you don't, your insurer may not cover claims for damage or theft. Your premiums could increase or decrease, depending on where your child attends school.
By Sandra Block
WHEELS: If you gotta have a car, try one of these
There's still some wisdom in not having a car at college, because there's seldom a handy, safe, cheap place to park. But that's too practical a notion for many college-bound kids, so an alternative is to drive something that's safe, reliable and cheap.
Nothing about college is cheap, of course, so we're using the term relatively and focusing on used cars instead of new ones.
A good starting point is Consumer Reports magazine's April auto issue (or www.consumerreports.org, for a fee). The most valuable item is a list of used cars to avoid for their poor reliability.
In addition, the magazine picks used vehicles that it believes have good emergency handling and braking, good crash-test scores, aren't likely to break, are powerful enough to pass and merge safely but not enough to invite irresponsibility and are big enough to provide heft in a crash.
"Unfortunately for the kid who's drooling, we don't recommend high-power, pure sports cars, and we don't recommend large SUVs and pickups, because they tend to roll over more easily," says Consumer Reports auto editor Rik Paul, formerly with Motor Trend
Here's the Consumer Reports list of best used cars for teen drivers:
Lexus RX300; Subaru Forester 2.5X; Ford Escape XLT (V-6); Mazda Tribute LX (V-6); Toyota Avalon XLS; Honda Accord EX (4-cylinder); Toyota Camry LE, XLE; Nissan Altima 2.5 S; Mazda6; Honda Civic EX; Toyota Corolla LE.
Not on the official list, but good bets for two-seater die-hards: Mazda Miata, Toyota MR2.
Detroit is sadly underrepresented, making it tough for fans of U.S. brands.
If it's any comfort, the Mazda Tribute is a Ford Escape with Mazda tweaks. Ford controls Mazda, so the Mazda6 is almost a Ford. And the Toyotas and Hondas are built in the USA or Canada.
Mazda6 probably is the best blend of attributes. It has the four-door-sedan practicality you'd get in a similar-size Accord or Camry, but has a hipper image and is more fun to drive.
By James R. Healey
CREDIT CARDS: Make sure child knows basics, consequences
Credit cards are a fact of life for college students. More than half of college freshmen, 54%, carried a credit card in 2001, according to the most recent Nellie Mae study of undergraduate card usage.
Graduating students had an average $20,402 in combined student loan and credit card balances.
But students need to know the basics about using credit cards, including fees and interest rates, says Laura Levine, executive director of the JumpStart Coalition for Personal Financial Literacy.
If they abuse credit, they'll start careers hobbled by debt. If they've tarnished their records, they might have trouble renting a home or getting insurance.
Some options to consider:
•A credit card on the parents' account. Parents get the bill and can track spending. There is no risk that students will get hit with late payment fees. But they won't build a credit history, says Robert McKinley, CEO of industry tracker CardWeb.com.
"It's not a reflection of the real world," adds Ken McEldowney, head of Consumer Action.
•A secured credit card. The credit line is guaranteed by money in a bank account. It's a way for students to get used to credit and paying bills on their own, but with limited ability to get into debt.
•A typical unsecured credit card. Usually, the credit limits are low for students, allowing them to build a credit record. But students risk running up big debts and damaging their credit.
•Non-credit card options. Parents can set up a joint checking account with a debit card. Or they can fund a prepaid card. There are no interest costs and no risk of hurting a credit record.
But it can be difficult to make some transactions, such as renting a car.
The key is knowing your child. "For a lot of students, college is their first taste of freedom, and it's about excess," says Susan Hirshman, a managing director at J.P. Morgan Fleming Asset Management.
Help your kids set boundaries on spending, and talk to them about budgeting, Hirshman says.
By Christine Dugas


text:
How mutch does this cost?
the basic package (with the free broadband that comes with the TV package) has a download limit, if you go over this it's not a huge deal, they will switch package which is £7.50. i think they onl...
Read More »
How mutch would this cost (uk).?
Sorry Timbo, but you are completely wrong on this one. There are plenty of companies in the U.K. who hire tanks as limos
I want to buy a train ticket to leave friday and return sunday is this possible and does it cost more?
No, you'd need two singles (because Alfreton is close to Sheffield there are no period returns - only )It's about £18 for an adult for two singles, you can buy at the station before travel.Edit 1@...
Read More »
How mutch is road tax chrysler pt cruiser in the uk?
Find one:Use the 'Vehicle enquiry' tab on the left of this formhttps://www.taxdisc.direct.gov.uk/EvlPor…enter the registration and vehicle make and it will tell you the taxEarly ones will be £22...
Read More »
How mutch is it to have a pisston ans barrel on a ped changed?
for a good quality big bore kit (70cc) its about £140 if you do it yourself however its much cheaper if you get a standard size kit and cheaper still if you can get a piston &rebore hope this help...
Read More »


text:
The Beatles - Across The Universe (Karaoke Version) MP3
The Beatles - Across The Universe (Karaoke Version)
Across the Universe - by Tamara Laporte (original by The Beatles) MP3
Recorded this version of Across the Universe many moons ago but never did a video for it. So here I'm lip-synching to my own voice. Ha! Hope you like, you can ...
The Beatles - Across The Universe // Cover By Analuz MP3
Download no 4shared: http://www.4shared.com/mp3/vl1ebttzce/Analuz_-_Across_The_Universe__.html? Em breve no Spotify, e no iTunes para download free.
Across the universe - RRSO symphony orchestra MP3
Instrumentales the Beatles.
Rufus Wainwright - Across The Universe MP3
Music video by Rufus Wainwright performing Across The Universe. (C) 2002 Geffen Records.
Across The Universe
Rufus WainwrightWords are flowing out like endless rain into a paper cup They slither while they pass, they slip away across the universe Pools of sorrow waves of joy are drifting through my opened [...]
Across The Universe - John Lennon, Paul McCartney. The Beatles. (Let It Be) (Cover By Andrew Ryan) MP3
My take on one of my favourite John Lennon songs during his time in The Bealtes. John Lennon was such an inspiration to us all, and a huge inspiration on me.
Fiona Apple - "Across The Universe" MP3
Uncut version. Song included in the movie "Pleasantville".
Let It Be (Across The Universe) MP3
When I find myself in times of trouble Mother Mary comes to me Speaking words of wisdom, let it be And in my hour of darkness She is standing right in front of ...
Across the universe- all we need is love MP3
I love this 3 minutes... would love to sing all the world you are for me I'll try, and if I could have started singing before I could do even better... I'd love to hear your ...
Across the Universe - Hey Jude. MP3
One of my favorite songs. [No infringement intended] Hey Jude, don't make it bad take a sad song and make it better Remember to let her into your heart Then ...
Karaoke Let It Be - Across The Universe * MP3
Download MP3: http://www.karaoke-version.com/mp3-backingtrack/across-the-universe/let-it-be.html Sing Online: ...
Don't Let Me Down (Across The universe) MP3
Don't let me down, Don't let me down Don't let me down, Don't let me down Nobody ever loved me like he does Oooo he does... yes, he does And if somebody ...
Across the Universe - I want to hold your hand MP3
T.V. Carpio singing I Want To Hold Your Hand written by the Beatles from the film "Across the Universe".
Casilda Santamaría - Across The Universe MP3
Canción solidaria en colaboración con la Fundación Raíces, para simbolizar la unión entre niños y niñas de todo el mundo, sin distinción de etnias, razas o ...
Across the Universe - I Want You She's So Heavy - Joe Anderson MP3
Across the Universe I Want You She's So Heavy Joe Anderson.
Remix: Watch Across the Universe with me (Re-Arranged Universe Mix) MP3
First RaldyV Remix of 2013!! Download free here! http://raldyvargas.bandcamp.com/track/watch-across-the-universe-with-me-raldyv-re-arranged-universe-mix ...
Dear Prudence (Across The Universe) MP3
Dear Prudence, won't you come out to play? Dear Prudence, greet the brand new day The sun is up, the sky is blue It's beautiful and so are you Dear Prudence ...
Across the universe - Being For The Benefit of Mr. Kite - (Legendado) MP3
Across the universe - Mr. Kite - (Legendado)
Laibach - Across the Universe MP3
Laibach - Across the Universe.
Thaykhay / Version / 2013/ full album MP3
Thaykhay Download this album for free in: http://www.thaykhay.com/ Save a prayer : Duran Duran Under pressure : Queen Bowie Nutshell : Alice in Chains ...


text:
You gotta love the folks over at HP for creating these new digital cameras that help make you look skinnier. Even though I can't see a big difference in the before and after effect that HP shows on their site trying to slim down a rotund couple with the digital camera's slimming feature, I'm all for anything that doesn't put weight on me, but claims to do the reverse.
And it's cool how you can photoshop your stuff right on the camera itself, as opposed to my current method of downloading my pics to my PC then seeking out whatever free photoshopping software will do the trick before I remember that I own HP Image Zone.
Anyhoo, these are all the digital camera that contain the slimming feature, according to HP's site: » HP Photosmart R967 digital camera » HP Photosmart R927 digital camera » HP Photosmart R827 digital camera » HP Photosmart R727 digital camera » HP Photosmart R725 digital camera » HP Photosmart M627 digital camera » HP Photosmart M527 digital camera
And here they all are on sale at Amazon, though not all the Amazon descriptions specifically list "slimming feature" like HP's site does. Anyhoo, read all the feedback from the folks who love and hate them and make your own decision. I'll be looking up some other digital camera that my friend is all hot over...
Bookmark or blogroll me at http://www.paulamooney.blogspot.com/ and drop back in some day to see what I'm up to next. Blessings and love, Paula
digital,camera,makes,you,look,skinny ,slimming ,weight ,takes ,off ,Photosmart ,R967 ,
R927 ,R827 ,R727 ,R725 ,M627 ,M527 ,snapfish ,free ,pics ,photos


text:
Another weirdo taste of the Man Man frontman's Use Your Delusion solo LP.
Album Reviews
Honus Honus shares new song "The Stripper Has No Navel" — listen
by Ben Kaye
on October 14, 2016, 3:33pm
Man Man frontman Honus Honus goes solo on new single "Oh No!" — listen
A summery slice from the eccentric songwriter's debut solo effort, Use Your Delusion.
by Ben Kaye
on September 19, 2016, 1:27pm
FYF Fest 2014: From Worst to Best
Forget the whining. There was plenty to like about FYF 2014.
on August 26, 2014, 9:15am
Toronto Urban Roots Festival Has It All Figured Out
Jeff Cohen provides an excellent lesson to festivals worldwide.
by Nick Freed
on July 07, 2014, 1:30pm
Yeasayer's Anand Wilder pens his own musical, Break Line, featuring star-studded cast
The all-star cast includes members of Vampire Weekend, Dirty Projectors, Chairlift, and many more.
on May 28, 2014, 11:45am
Shaky Knees Music Fest 2014: Top 12 Sets + Photos
Awards for: Best Headliner, Worthiest Rain Set, Most Refreshing, and more.
by Michael Roffman,Josh Terry and Max Blau
on May 12, 2014, 3:59am
Musicfest NW reveals 2014 lineup
Spoon, HAIM, tUnE-yArDs, Future Islands, Phantogram, and more.
by Alex Young
on April 29, 2014, 6:13pm
Listen: "The Super Bowl Shuffle" reimagined by Jim James, Les Savy Fav, and Lil Bub
Benefiting the Reading Is Fundamental charity.
on January 21, 2014, 12:30pm
Shaky Knees reveals 2014 lineup: The National, Modest Mouse, and Spoon
Plus, Ed Sharpe, Conor Oberst, Local Natives, and Jenny Lewis.
by CoS Staff
on January 15, 2014, 11:04am
Anderson Cooper dedicates an entire segment to Man Man and their Wolf Blitzer song
on September 20, 2013, 1:15pm
Album Review: Man Man – On Oni Pond
The Philly boys are as weird as ever, but with a newfound subtlety and focus.
on September 10, 2013, 12:00am
Man Man's new single "End Boss" is perfect video game music
Our second taste of On Oni Pond.
on August 22, 2013, 1:21pm


text:
Incoming freshmen are beginning their visits to MSU to attend the Academic Orientation Program (AOP). While they're on campus, students learn more about their major, all the resources available to them as MSU and CAS students, and college life in general.
According to the MSU Admissions Office, more than 30,000 students have applied to the university thus far. Around 7,900 will be admitted. Currently, there are 341 incoming freshmen and 70 transfer students signed up for CAS's AOP this summer.Students attending orientation stay overnight in Case Hall to experience cafeteria food and dorm life, attend a few different seminars, tour the campus and their college, meet with their adviser and schedule their classes.
When incoming students who have declared a CAS major visit the building, an AOP staff member helps them use "Schedule Builder" to enroll in courses for their freshmen year. Most students will be advised to enroll in a mix of university requirements and courses for their major.
AOP staff members serve as a great resource for incoming freshmen because all of them are current MSU students. They not only answer questions, but suggest courses and organizations based on the student's interest, and help them to see how much MSU has to offer.
James Adams, an incoming freshman from Harbor Springs, Mich., who plans to double major in journalism and advertising, said, "So far, it's been great. Everyone is so friendly, and I'm excited to come here."
Share via these networks:


text:
The dietary bioflavonoid quercetin synergizes with epigallocathechin gallate (EGCG) to inhibit prostate cancer stem cell characteristics, invasion, migration and epithelial-mesenchymal transition
- First Online:
- Received:
- Accepted:
- Citations
- 96k Downloads
Much attention has been recently focused on the role of cancer stem cells (CSCs) in the initiation and progression of solid malignancies. Since CSCs are able to proliferate and self-renew extensively due to their ability to express anti-apoptotic and drug resistant proteins, thus sustaining tumor growth. Therefore, the strategy to eradicate CSCs might have significant clinical implications. The objectives of this study were to examine the molecular mechanisms by which epigallocathechin gallate (EGCG) inhibits stem cell characteristics of prostate CSCs, and synergizes with quercetin, a major polyphenol and flavonoid commonly detected in many fruits and vegetables.
Our data indicate that human prostate cancer cell lines contain a small population of CD44+CD133+ cancer stem cells and their self-renewal capacity is inhibited by EGCG. Furthermore, EGCG inhibits the self-renewal capacity of CD44+α2β1+CD133+ CSCs isolated from human primary prostate tumors, as measured by spheroid formation in suspension. EGCG induces apoptosis by activating capase-3/7 and inhibiting the expression of Bcl-2, survivin and XIAP in CSCs. Furthermore, EGCG inhibits epithelial-mesenchymal transition by inhibiting the expression of vimentin, slug, snail and nuclear β-catenin, and the activity of LEF-1/TCF responsive reporter, and also retards CSC's migration and invasion, suggesting the blockade of signaling involved in early metastasis. Interestingly, quercetin synergizes with EGCG in inhibiting the self-renewal properties of prostate CSCs, inducing apoptosis, and blocking CSC's migration and invasion. These data suggest that EGCG either alone or in combination with quercetin can eliminate cancer stem cell-characteristics.
Since carcinogenesis is a complex process, combination of bioactive dietary agents with complementary activities will be beneficial for prostate cancer prevention and/ortreatment.
Analysis of Variance
Breast cancer resistant protein
Embryonic stem cells
Multidrug resistance
Phosphatase and Tensin Homolog Deleted on Chromosome 10
Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis
Tris Buffer Saline
Prostate cancer currently accounts for 29 percent of all new cancer diagnoses in men. It is estimated that more than 27,000 U.S. men died of this disease in 2009, and this incidence is likely to increase as the male population ages [1, 2]. The development and progression of prostate cancer is a slow and complex process that involves multiple steps of tumorigenic transformation differentially modulated by endocrine, nutritional and perhaps inflammatory/immune factors. To date, the molecular mechanisms that mediate the initiation and progression of prostate cancer remain poorly understood. Preventive strategies for prostate cancer require considerable new knowledge about the mechanisms underlying the pathogenesis and progression of the disease. Cancer stem cells (CSCs) are generally thought of as self-renewing cells that are able to reinitiate a tumor for several generations and can give rise to a spectrum of differentiated cells [3, 4, 5]. CSCs, like normal stem cells, are also more likely to express antiapoptotic and drug-resistance genes, making them impervious to most anticancer therapeutics [6, 7, 8, 9, 10, 11, 12, 13]. In order to eradicate a tumor and prevent recurrence, it is imperative that cancer stem cells be specifically targeted.
Cancer stem/progenitor cells may exhibit characteristics similar to normal stem cells. CSCs have limitless potential for self-renewal and can efficiently form tumors in immunodeficient mice that recapitulate the heterogeneity observed in original tumors [14, 15, 16, 17]. Recently, CSCs have been described in several human tumors including breast, gastrointestinal, lung, prostate, brain, and melanoma on the basis of their clonogenic efficiency in vitro and ultimately tumorigenicity in vivo [15, 16, 18, 19, 20, 21]. The identification and characterization of CSCs might have enormous clinical implications: for instance, it is has been shown that CSCs might survive chemo- as well as radiotherapy [12, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31], due to the preferential expression of resistance molecules or activation of specific signaling pathways. Therefore, understanding the mechanisms of drug resistance and development of novel strategies to kill CSCs are urgently needed.
Human prostate epithelial (HPE) exhibit stem cell characteristics, expressing embryonic stem cell markers, including Oct-4, Nanog, and Sox-2, in addition to the early progenitor cell markers CD133, CD44, and nestin. HPET cells do not express p63 and AR, similar to other reports on prostate stem cells [32, 33]. Most importantly, clonally derived HPET cells are capable of reconstituting the original prostate tumor from which they were derived and retain the ability to differentiate into basal, luminal, and neuroendocrine epithelial cell types of the prostate in vivo. CD44 is a basal cell marker that has been studied as a marker for human prostate CSCs [34, 35].
Examination of human prostate cancer cell lines and xenografts indicate that the CD44+ population is more proliferative, clonogenic, tumorigenic, and metastatic than CD44- cells. Prostate cancer cell lines sorted for high expression of CD44 have been associated with enhanced expression of "stemness" markers including BMI, β-catenin, SMO, and Oct 3/4 [36, 37, 38]. Moreover, CD44+α2β1+CD133+ subpopulations obtained from human tissue have enhanced capacity for in vitro serial passaging.
Epithelial-mesenchymal transition (EMT) induction in cancer cells results in the acquisition of invasive and metastatic properties [13, 29, 39, 40, 41]. Recent reports indicate that the emergence of CSCs occurs in part as a result of EMT, for example, through cues from tumor stromal components. CSCs and EMT-type cells, which shares molecular characteristics with CSCs, have been believed to play critical roles in drug resistance and early cancer metastasis as demonstrated in several human malignancies including prostate cancer [13, 29, 39, 40, 41]. Thus, the discovery of molecular knowledge of drug resistance and metastasis in relation to CSCs and EMT in prostate cancer is becoming an important area of research, and such knowledge is likely to be helpful in the discovery of newer drugs as well as designing novel therapeutic strategies for the treatment of prostate cancer with better outcome.
Epidemiological and dietary intervention studies in animals and humans have suggested that diet-derived phenolics, in particular the flavonoids, may play a beneficial role in inhibiting, reversing or retarding tumorigenesis in many types of cancers, including prostate cancer [42, 43, 44, 45, 46, 47]. Flavonoids are known to possess anti-inflammatory, anti-oxidant, anti-allergic, hepato-protective, anti-thrombotic, anti-viral and anti-carcinogenic activities [42, 43, 44, 45, 46, 47]. These activities of flavonoids are thought to be mediated by interfering with a large number of mammalian enzymes, such as detoxifying enzymes that are involved in cell division and proliferation pathways [42, 45, 48, 49, 50]. Among the flavonoids, quercetin (3,3',4',5,7-pentahydroxyflavone) is a naturally occurring flavones, and is a component of most edible fruits and vegetables, with the highest concentrations being found in onions, apples, and red wine [42, 45, 51]. Several studies have shown that quercetin has a broad range of pharmacological properties that include antioxidant and anti-inflammatory activities [52]. Quercetin treatment has been associated with selective antiproliferative effects and induction of cell death, predominantly through an apoptotic mechanism, in cancer cell lines but not in normal cells [53, 54, 55, 56]. The anti-proliferative effect of quercetin is believed to be exerted by producing arrest in the G1 phase of the cell cycle or through interaction with cell cycle-regulated proteins, like cyclin D1 and CDK4 [57, 58, 59]. Quercetin also has been shown to induce apoptosis by releasing cytochrome c and activating caspase-9 and caspase-3 [60, 61, 62]. Moreover, quercetin is believed to be a potential PI3K inhibitor, an enzyme involved in the pivotal cell survival pathway [63, 64]. Interestingly, quercetin has been shown to enhance the anticancer activities of several chemotherapeutic drugs and inhibit the expression of P-glycoprotein [65, 66, 67, 68, 69, 70]. Therefore, the ability of quercetin to prevent and/or to retard tumor growth is probably a multifunctional effect. Although the underlying mechanisms governing these effects are not yet fully understood, the available evidence collectively indicates that quercetin may be of therapeutic benefit in clinical settings, suggesting its potential use as an anticancer agent or an adjunct to current cancer therapies.
Epigallocathechin gallate (EGCG) from tea has gained recognition as important chemopreventive agent and as modulators of tumor cell response to chemotherapy [71, 72, 73, 74, 75]. EGCG downregulates Pg-P and BCRP in a tamoxifen resistant MCF-7 cell line [76]. In vitro and in vivo studies have demonstrated that EGCG affect a wide array of molecular pathways, resulting in inhibition of cell growth, invasion, angiogenesis, and metastasis [77, 78]. In prostate cancer, EGCG alter numerous intracellular pathways, including inhibition of ERK1/2- and Akt-mediated signaling, inhibition of PMA-dependent PKC activation, alteration of Bcl-2 family members ratio, and activation of caspases [79, 80, 81, 82, 83]. We have recently demonstrated that EGCG caused growth arrest at G1 stage of cell cycle through regulation of cyclin D1, cdk4, cdk6, p21/WAF1/CIP1 and p27/KIP1, and induced apoptosis through generation of reactive oxygen species and activation of caspase-3 and caspase-9 [77, 78, 84]. EGCG inhibited expressions of Bcl-2 and Bcl-XL and induced expressions of Bax, Bak, Bcl-XS and PUMA [77, 78, 84]. Furthermore, the activities of Ras, Raf-1 and ERK1/2 were inhibited, whereas the activities of MEKK1, JNK1/2 and p38 MAP kinases were induced by EGCG. Inhibition of cRaf-1 or ERK enhanced EGCG-induced apoptosis, whereas inhibition of JNK or p38 MAP kinase inhibited EGCG-induced apoptosis. EGCG inhibited the activation of p90 ribosomal protein S6 kinase, and induced the activation of cJUN [77, 78, 84]. Xenograft and TRAMP models have shown that green tea or EGCG can decrease the tumorigenic potential of prostate cancer (7-10). These studies demonstrate that EGCG induces growth arrest and apoptosis through multiple mechanisms. Overall, these findings suggest that green tea and its constituents can be used for chemoprevention to target cancer stem cells.
The objectives of our study were to characterize prostate cancer stem cells, and examine the molecular mechanisms by which quercetin enhances the inhibitory effects of EGCG on self-renewal capacity of prostate cancer stem cells.
CD44+ and CD133+ CSCs can be isolated from PC-3 and LNCaP cells
EGCG inhibits the growth of cancer stem cells isolated from human prostate cancer cell lines
EGCG inhibits the formation of primary and secondary tumor spheroids and cell viability of human prostate cancer stem cells
Since EGCG inhibited the growth of tumor spheroid and cell viability of CSCs, we next sought to examine the effects of EGCG on colony formation. Prostate CSCs were grown in agar, and treated with various doses of EGCG for 3 weeks. At the end of incubation period, colonies were counted. EGCG inhibited the growth of colonies in a dose-dependent manner (Fig. 3C). These data suggest that EGCG can be effective in inhibiting the self-renewal capacity of prostate cancer stem cells.
Since CSCs appear to play a significant role in early metastasis [13, 41, 88, 89], we sought to measure the effects of EGCG on invasion and migration of CSCs. EGCG inhibited cell migration and invasion (Fig. 3D and 3E). These data suggest that EGCG can be a useful agent in targeting prostate cancer stem cells.
Inhibition of Nanog enhances the effects of EGCG on spheroid formation by human prostate cancer stem cells
EGCG inhibits the expression of XIAP and Bcl-2 and induces caspase-3 activation in human prostate cancer stem cells
EGCG inhibits the expression of epithelial-mesenchymal transition marker (EMT) in human prostate cancer stem cells
Quercetin enhances the effects of EGCG on cell viability in spheroids, colony formation, apoptosis, migration and invasion by prostate cancer stem cells
Our study demonstrates, for the first time, that cancer preventive effects of EGCG and quercetin can inhibit the self-renewal capacity of prostate cancer stem cells. Our data are agreement with others who have demonstrated that prostate cancer cell lines contain a small population of cells having stem cell characteristics. EGCG can synergies with quercetin to inhibit the self-renewal capacity of prostate CSCs expressing CD44+α2β1+CD133+. EGCG induces apoptosis by activating capase-3/7, inhibiting Bcl-2, survivin and XIAP. EGCG inhibits the expression of vimentin, slug, snail and nuclear β-catenin which were accompanied by the inhibition of TCF-1/LEF reporter activity. The inhibition of EMT-related genes by EGCG suggests the blockade of signaling involved in early metastasis. Interestingly, quercetin synergizes with EGCG in inhibiting self-renewal capacity of prostate CSCs and their migration and invasion. These data suggest that EGCG either alone or in combination with quercetin can eliminate cancer stem cell-characteristics. Since carcinogenesis is a complex process, combination of bioactive dietary agents with complementary activities will be beneficial for prostate cancer prevention and/or treatment.
Most carcinomas comprise of a heterogeneous cell population with marked differences in their ability to proliferate and differentiate as well as their ability to reconstitute the tumor upon transplantation. Recent studies indicate the presence of a small, stem-like cell population in several human cancers that is crucial for the tumor repopulation. In a recent study, it has been demonstrate that five out of six prostate cancer cell lines formed clear holo-, mero-, and paraclones [87]. DU145 holoclones were maintained in culture for several passages, which is indicative of self-renewal ability. A small fraction (0.01%) of CD133+ cells was detected in DU145 cell lines [87]. CD133+ cells; however, like DU145 BCRP+ (0.15%) cells, they were not more clonogenic, and they did not show more holoclone formation than the marker-negative cells or unselected cells. Immunohistochemistry revealed alpha2-integrin and BCRP as potential stem cell markers and CK5 with the combination of CK18 to distinguish transient amplifying cells [87]. Similarly, in the present study, the proportions of CD133+ cells in PC-3 and LNCaP cell lines were 3.8% and 0.9%, respectively; and these cells were able to form primary and secondary spheres. This led to the hypothesis that the entire population of tumor cells might arise from a rare subpopulation of putative cancer stem/progenitor-like cells, also known as tumor-initiating cells or cancer stem cells [20, 87, 107, 108]. CSCs share principle characteristics with adult stem cells namely self-renewal, high proliferative potential, clonogenicity, and multipotency. In addition, they have the ability to reproducibly form the same tumor phenotype as in the patient and to undergo differentiation into non-tumorigenic cells [87, 109, 110]. TICs were first isolated from patients with hematologic malignancies in which a few cells could initiate a new tumor [111]. During the past few years, CSCs were also identified and isolated from solid tumors such as breast, brain, colon, pancreatic, and prostate tumors [14, 15, 112, 113, 114, 115, 116, 117]. The cancer stem cell hypothesis has provided a paradigm shift in our understanding of carcinogenesis, metastasis, and tumor biology. The identification of CSCs has important implications in the way cancer treatment should be conceived and future therapeutic approaches will be designed. Whether the subpopulation of CSCs is involved in the formation of distant metastases, tumor dormancy and therapy resistance has remained poorly understood.
The molecular mechanism by which CSCs (and the supportive stroma) play roles in the formation of distant metastases has remained largely elusive. The development of more effective cancer therapies in advanced prostate cancer may, thus, benefit from the outcome of new studies and require selective targeting of this specific subpopulation of metastasis-initiating cells. In prostate cancer, innovative studies have led to the identification of CD44+/α2β1high/CD133+ prostate cancer stem cells [20, 118]. Because of the observed heterogeneity in prostate cancer, the use of single-cell markers for the selection, characterization/identification, and functional evaluation of stem/progenitor-like prostate cancer cells has been a major impediment and the reliability of cell surface markers such as CD133 as the sole way to isolate TICs remains controversial to date [108, 119]. Deregulation of ALDH enzyme activity is implicated in the pathophysiology of various hematologic and epithelial cancers [120]. The introduction of FACS-based viable cell sorting for ALDH activity (ALDEFLUOR assays) in tumor biology has further substantiated a role of ALDHhi subpopulations of cancer cells in carcinogenesis [114, 121, 122, 123]. High ALDH activity, as detected by the ALDEFLUOR assay, can thus be used as a functional marker to isolate TICs in several types of epithelial cancers, including those of breast, lung and colon [114, 121, 122, 123, 124, 125]. A recent study has demonstrated that high ALDH activity can be used to isolate human prostate cancer cells with significantly enhanced clonogenic and migratory properties in vitro as well as elevated tumor- and metastasis-initiating abilities in vivo [126]. The percentage of ALDHhi cells in prostate cancer cell lines also seems to be related to tumorigenicity and metastatic behavior.
Quercetin is a ubiquitous bioactive plant flavonoid that has been shown to inhibit cell proliferation in several cancer types. Quercetin has been shown to decrease Akt phosphorylation and survivin expression in prostate cancer cells [64, 127]. Studies investigating molecular mechanisms that underlie the inhibition of cell proliferation by quercetin demonstrated that a treatment including quercetin triggered numerous cellular events including DR5 upregulation [106, 128, 129, 130], p53 activation [131, 132, 133, 134], cell cycle arrest [58, 59], and induction of caspase-mediated apoptosis in cancer cells. Quercetin down-regulates the expression of Hsp90 which, in turn, induces growth inhibition and apoptosis in prostate cancer cells while exerting no quantifiable effect on normal prostate epithelial cells. Quercetin has also been shown to inhibit the expression of BCRP and MDR expression [66, 135, 136]. In our study, quercetin has been able to synergies with EGCG in inhibiting the self-renewal capacity of prostate CSCs, migration and invasion, and inducing apoptosis. Since quercetin is a MDR modulator, it can sensitize CSCs to anticancer drugs.
Recent evidence suggests a shared genomic fingerprint between embryonic stem cells, cancer cells, and cancer stem cells. Activation targets of Nanog, Oct4, Sox2 and c-Myc are more frequently overexpressed in certain tumors. In the absence of bona fide cancer stem cell lines, human embryonic stem cells, which have similar properties to cancer and cancer stem cells, have been an excellent model throwing light on the anticancer affects of various putative anticancer agents. Nanog, Sox2 and Oct4 are transcription factors which are essential to maintaining the pluripotent embryonic stem cell phenotype. Oct4 and Sox2 bind to the Nanog promoter in living mouse and human ESCs [137]. Nanog, Oct4 and Sox2 co-occupy and regulate their own promoters together with other developmental genes with diverse functions and collaborate to form an extensive regulatory circuitry including autoregulatory and feed-forward loops [137, 138, 139]. A high level of Nanog is a key regulator of ESC self-renewal and puripotency. Nanog-deficient ES cells and embryos lose their pluripotency [92]. Nanog overexpression leads to the clonal expansion of ES cells through circumvention of the LIF-dependent Stat-3 pathway and sustained Oct-4 expression levels [92, 140]. Genome-wide gene expression profiling shows that Nanog is expressed at high levels in testicular carcinoma in situ and germ cell tumors [141]. Positive correlations of Oct4, Nanog, or CD133 expression on tumor stage were shown on oral squamous cell carcinoma patient tissues [142]. In our study, inhibition of Nanog by shRNA enhanced the inhibitory effects of EGCG on tumor sphere formation and cell viability, suggesting its requirement for self-renewal of CSCs. Medulloblastoma-associated CSCs selected by serum-free medium with bFGF and EGF can form 3 D spheroids and display enhanced self-renewal and highly co-expressed stem cell genes (Oct4, Nanog, Nestin, and Musashi-1) as well as anti-apoptotic genes (Bcl-2 and Bcl-XL) [143]. These finding suggest that inhibition of Nanog and/or other pluripotent factors could be a novel strategy to kill CSCs.
EMT is an embryonic program in which epithelial cells lose their characteristics and gain mesenchymal features. Therefore, EMT might play a very important role during malignant tumor progression. Accumulating evidence suggest that transformed epithelial cells can activate embryonic programs of epithelial plasticity and switch from a sessile, epithelial phenotype to a motile, mesenchymal phenotype. Induction of EMT can, therefore, lead to invasion of surrounding stroma, intravasation, dissemination and colonization of distant sites. Under the cancer stem cell hypothesis, sustained metastatic growth requires the dissemination of a CSC from the primary tumor followed by its re-establishment in a secondary site. In the present study, EGCG inhibited the expression of EMT markers (expression of vimentin, nuclear β-catenin and TCF-1/LEF reporter activity) and also inhibited the transcription factors slug and snail, which are required for induction of EMT. The inhibition of EMT markers by EGCG suggests that it could inhibit early metastasis of prostate CSCs. Furthermore, quercetin will further enhance the biological effects of EGCG in inhibiting EMT and hence early metastasis.
In conclusion, we have demonstrated that EGCG inhibited self-renewal capacity of prostate CSCs, and these properties of EGCG were further enhanced by quercetin. EGCG inhibited the expression of transcription factors which are required for maintaining stem-cell pluripotency. Inhibition of Nanog could be considered as a novel strategy to enhance the biological effects of anticancer and chemopreventive agents or sensitize those cells which are resistant to chemotherapy or irradiation. Moreover, EGCG inhibited expression of proteins involved in the EMT, suggesting the blockade of signaling involved in early metastasis. Furthermore, combination of quercetin with EGCG had synergistic inhibitory effects on self-renewal and metastatic properties of prostate CSCs. These data suggest that EGCG either alone or in combination with quercetin can be used for the prevention and/or treatment of prostate cancer. However, further studies are needed to validate the combination of EGCG and quercetin in an appropriate in vivo model.
Antibodies against β-catenine, vimentin, slug, snail, GAPDH, XIAP, survivin, Bcl-2, and Nanog were purchased from Cell Signaling Technology, Inc. (Danvers, MA). EGCG and quercetin were purchased from LKT Laboratories, Inc. (St. Paul, MN). Enhanced chemiluminescence (ECL) Western blot detection reagents were from Amersham Life Sciences Inc. (Arlington Heights, IL). Terminal Deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL) assay kit was purchased from EMD Biosciences/Calbiochem (San Diego, CA). All other chemicals were purchased from Sigma-Aldrich (St Luis, MO).
Cell Culture
PC-3 and LNCaP cells were obtained from the American Type Culture Collection (Manassas, VA). Human prostate cancer stem cells (CD44+α2β1+CD133+) were from Celprogen Inc. (San Pedro, CA). CSCs were cultured in DMEM supplemented with 1% N2 Supplement (Invitrogen), 2% B27 Supplement (Invitrogen), 20 ng/ml human platelet growth factor (Sigma-Aldrich), 100 ng/ml epidermal growth factor (Invitrogen) and 1% antibiotic-antimycotic (Invitrogen) at 37°C in a humidified atmosphere of 95% air and 5% CO2.
Tumor Spheroid Assay
Spheroid forming assays were performed as described elsewhere [144, 145]. In brief, single cells were plated in six-well ultralow attachment plates (Corning Inc., Corning, NY) at a density of 1,000 cells/ml in DMEM supplemented with 1% N2 Supplement (Invitrogen), 2% B27 Supplement (Invitrogen), 20 ng/ml human platelet growth factor (Sigma-Aldrich), 100 ng/ml epidermal growth factor (Invitrogen) and 1% antibiotic-antimycotic (Invitrogen) at 37°C in a humidified atmosphere of 95% air and 5% CO2. Spheroid were collected after 7 days and dissociated with Accutase (Innovative Cell Technologies, Inc.). The cells obtained from dissociation were sieved through a 40-μm filter, and counted by coulter counter using trypan blue dye.
Soft agar colony assay
Cell suspensions (2,500 cells) were prepared using 0.4% Noble agarose (Becton Dickinson) and overlayed onto a 60-mm dish containing a solidified bottom layer of 0.6% agarose in medium. Once the top layer solidified, 1 ml of medium was placed on top of the cell layer. After treatment, plates were incubated for 3 weeks and colonies were counted by using microscopy.
Transwell Migration assay
For transwell migration assays, 1 × 105 prostate CSCs were plated in the top chamber onto the noncoated membrane (24-well insert; pore size, 8 μm; Corning Costar) and allowed to migrate toward serum-containing medium in the lower chamber. Cells were fixed after 24 hours of incubation with methanol and stained with 0.1% crystal violet (2 mg/mL, Sigma-Aldrich). The number of cells invading through the membrane was counted under a light microscope (40×, three random fields per well).
Transwell invasion assay
For invasion assay, 1 × 105 cells were plated in the top chamber onto the Matrigel coated Membrane (24-well insert; pore size, 8 μm; Corning Costar). Each well was coated freshly with Matrigel (60 μg; BD Bioscience) before the invasion assay. Cells were plated in medium without serum or growth factors, and medium supplemented with serum was used as a chemoattractant in the lower chamber. The cells were incubated for 48 hours and cells that did invade through the pores were removed by a cotton swab. Cells on the lower surface of the membrane were fixed with methanol and stained with crystal violet. The number of cells invading through the membrane was counted under a light microscope (40×, three random fields per well).
Viral production and infection
Nanog shRNA construct (pLKO.1-puro, Mission RNAi) was from Open Biosystems. Lentiviral-TOP-dGFP-reporter (pRRL.sin-18.ppt) has been described elsewhere [146]. Lentivirus was produced by triple transfection of HEK 293T cells. Viral supernatants were collected and concentrated by ultracentrifugation to produce virus stocks with titers of 1 × 108 to 1 × 109 infectious units per milliliter. Viral supernatant was collected for three days by ultracentrifugation and concentrated 100-fold [147]. Titers were determined on HEK293T cells. Human prostate CSCs were transduced with a mixture of viral particles and polybrene with two rounds of infections [147].
Lentiviral reporter assays
The enhanced d2-eGFP gene (Clontech) was cloned downstream of a LEF-1/TCF-responsive promoter (β-catenin reporter), containing three LEF-1/TCF binding motifs and a TATA box [148]. This cassette was then cloned into a self-inactivating lentiviral vector plasmid (pRRL.sin-18.ppt), and virus was produced as described above. For in vitro assays, transduced CSCs were plated at 1,000 cells per well in 96-well plates and treated with EGCG and/or quercetin. After incubation, CSCs were analyzed for GFP expression.
Caspase-3/7 Assay
Cells (3 × 104 per well) were seeded in a 96-well plate with 200 μl culture medium. Approximately 16 h later, cells were treated with various doses of EGCG with or without quercetin. Casapse-3/7 activity was measured by a fluorometer as per manufacturer's instructions (Invitrogen).
Western Blot Analysis
Western blots were performed as we described earlier [149, 150]. In brief, cells were lysed in RIPA buffer containing 1 × protease inhibitor cocktail, and protein concentrations were determined using the Bradford assay (Bio-Rad, Philadelphia, PA). Proteins were separated by 12.5% SDS/PAGE and transferred to membranes (Millipore, Bedford, MA) at 55 V for 4 h at 4°C. After blocking in 5% nonfat dry milk in TBS, the membranes were incubated with primary antibodies at 1:1,000 dilution in TBS overnight at 4°C, washed three times with TBS-Tween 20, and then incubated with secondary antibodies conjugated with horseradish peroxidase at 1:5,000 dilution in TBS for 1 hour at room temperature. Membranes were washed again in TBS-Tween 20 for three times at room temperature. Protein bands were visualized on X-ray film using an enhanced chemiluminescence detection system.
Statistical Analysis
The mean and SD were calculated for each experimental group. Differences between groups were analyzed by one or two way ANOVA, followed by Bonferoni's multiple comparison tests using PRISM statistical analysis software (GrafPad Software, Inc., San Diego, CA). Significant differences among groups were calculated at P < 0.05.
We thank our lab members for critical reading of the manuscript.
We thank Wenyu Zhu for technical help. This work was supported in part by the grants from the National Institutes of Health (R01CA125262 and RO1CA114469), and Kansas Bioscience Authority.
We thank our lab members for critical reading of the manuscript. We thank Wenyu Zhu for technical help. This work was supported in part by the grants from the National Institutes of Health (R01CA125262 and RO1CA114469), and Kansas Bioscience Authority.
Copyright information
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text:
Deep South Tater Tot Casserole
Be the first to review
READY IN: 1hr 30mins
Recipe by 5nolans
The original recipe is from Deep South Dish.com. I use a 9x13 casserole dish and add about 2 cups diced ham, a whole pound of smoked sausage (chopped) and a whole cup of cheese (separated).
See Discussion:
Ingredients Nutrition
- 283.49 g can cream of mushroom soup or 283.49 g can chicken soup
- 236.59 ml milk
- 7.39 ml olive oil
- 118.29 ml of chopped vidalia onions or 118.29 ml other sweet onion
- 59.14 ml of chopped green bell pepper
- 453.59 g ground beef
- 4.92 ml of minced garlic
- 226.79 g smoked sausage, chopped (optional)
- 14.79 ml fresh basil
- 2.46 ml dried thyme
- 1.23-2.46 ml cajun seasoning
- kosher salt
- freshly cracked black pepper, to taste
- 118.29 ml shredded cheddar cheese, divided
- 453.59 g frozen tater tots
Directions See How It's Made
- Preheat oven to 350 degrees F. Butter or spray a 9 x 9 inch casserole dish with non-stick spray; set aside. Whisk together the cream soup and milk until well blended; set aside.
- Add olive oil to a large skillet and saute the onion and bell pepper until softened but not browned, about 5 minutes. Stir in the ground beef and brown; drain if needed and return to the skillet. Add the minced garlic, cook and stir another minute. Add in the chopped smoked sausage and cook about 3 minutes. Stir in the basil, thyme, Cajun seasoning; taste and add salt and pepper only as needed. Transfer meat mixture to the prepared casserole dish and sprinkle with 1/4 cup of the cheddar cheese. Evenly pour the soup mixture over the top. Arrange the tater tots on top and bake uncovered at 350 for 45 minutes to 1 hour, or until potatoes are nicely browned and crisp. Sprinkle the remaining 1/4 cup of shredded cheese on top and return to the oven just until cheese is melted.
- Serve immediately with a green vegetable or a mixed garden salad.


text:
This will be just about the easiest 1,000 United MileagePlus miles you'll earn for a while: Sign up to receive text messages from Visa Offers (via Thanks Again) and you'll get 1,000 United miles credited to your account in about, oh, 8-12 weeks or so. Here's how:
– Go to Thanks Again
– Register for a new account
– When done registering, log in to your account
– Go to the My Account section and click on "Click Here to Join Thanks Again Offers"
– You'll see that it says you'll get 1,000 miles for signing up for Visa Offers.
Yes, you'll have to give your credit card (so they can track your spending) and your phone number (so they can receive texts). But you can opt out, apparently any time after signing up.
If you're already a Thanks Again member (which I was, for who-can-possibly-remember-what-reason), you can only earn the miles if you originally came through that United link. Or, of course, you can create a new account with a different username (just saying…)
Yes, text offers can be annoying. If it's not worth 1,000 miles to you, don't enroll. But this was an easy 1,000 miles, and if it's annoying I'll unsubscribe.
(Thanks, Slickdeals)


text:
At just 38, the founder of low-cost carrier easyJet and a raft of "easy" companies takes to the annual pow-wow of business, political and cultural leaders like a fish to water.
In an interview with AFP, the travel magnate described activities on three continents but restricted to one sector.
Haji-Ioannou targets younger, independent clients who can get themselves to his ship, albeit preferably via his airline, which has led a revolution in the European travel industry by providing no frills — "very little hand-holding," as he put it — in order to cut costs.
The easyCruise ship was full within a couple of months of its launch, and after plying the Mediterranean headed during the northern hemisphere winter to the Caribbean.
Next stop is Miami, where Haji-Ioannou plans to take on the heavyweights, Carnival and Royal Caribbean. "In cruise industry terms it's the lion's den," he joked.
Meanwhile, he said, he was upgrading the restaurant on board the flagship, after finding passengers wanted an occasional break from burgers, pizzas and chips. "We're moving to a more upscale menu."
Custom-designed plans for a second, 500-passenger vessel can be seen on the Internet. Casinos, on the other hand, are not in the cards, as is the case in southern Cyprus, where Haji-Ioannou's mother was born.
Is easyCruise his baby? "Unfortunately, I've got another 14 of them," he laughed, and his business card lists what seems like "easy" everything, from Money and Jobs to Music and Value.
Getting back to his first big success, Haji-Ioannou said of consolidation in the airline industry that: "I think there will be five airlines that matter in Europe.
"British Airways, Air France, Lufthansa, Ryanair and easyJet, in no particular order."
The first three benefit from large domestic markets, while the latter two were low-cost pioneers on the continent.
Arch-rival Ryanair has said it will begin to charge for check-in baggage, causing easyJet's biggest shareholder to quip: "It's fascinating what they're getting away with."
He pressed for details and checked off potential flaws, while admitting the idea had already crossed his mind.
"I can see two huge bottlenecks that would eliminate any efficiency gains," the first being check-in delays if passengers paid at the counter.
If Internet payments were favored, he said, some people would board with oversized bags, leading to delays and disputes when cabin staff enforced the new rule.
"It will slow down the aircraft which is a much more expensive asset," he warned, as well as overload already inadequate overhead bins.
"Either he (Ryanair boss Michael O'Leary) has found a solution which I haven't thought of, or the whole thing is going to be a complete nightmare."
Judging the battle between aircraft makers Airbus and Boeing, Haji-Ioannou wished them both well, as it would keep his prices competitive.
He agreed with Boeing's analysis that passengers prefer to fly from one point to another on smaller planes than via huge "hub-and-spoke" airports that favor jets like the future Airbus A380 superjumbo.
But noting that Boeing will have to phase out its venerable 747 at some point, he added: "I think there will be enough orders for the A380 for Airbus to justify the investment" of up to 11.45 billion euros (13.9 billion dollars).
Finally, those with their feet on the ground can experience the group's "cheap-chic" business model at hotels in London and Switzerland, with more to come in Britain, Europe and the Middle East.
An easyBus even takes passengers to easyJets at Luton, north of London.
The big boss amiably admitted: "It's a bit more difficult to argue that easyBus is chic. It's a bus.
"Nobody is trying to make an experience out of a bus ride."


text:
Video: Ball State Student Wins Free Tuition After Making This Half-court Shot
Freshman Markus Burden won't have to pay for his spring semester (valued at over $11K) at Ball State, thanks to his halfcourt miracle shot below. According to the Star Press, Burden was chosen at random by the school during a Thursday night Welcome Week activity at Worthen Arena.
The shot and celebration are worth watching multiple times:


text:
Sabka sath, sabka vikas, this slogan of BJP Government has raised many questions on the party, not because of its meaning, but actually because of the question 'kiska sath, kiska vikas'. As of now, the present tag on Modi government is of Corporate ki sarkar, and Prime Minister Modi's image is most affected with any action that validates this accuse.
The so called involvement of foreign affairs minister Sushma Swaraj has put a Big Question Mark on Government's role. As per reports, she went out of her way to get Lalit Modi, travel documents from the British government because his passport had been invalidated by the Indian government for his alleged violation of Foreign Exchange Management Act (FEMA). In connection with the case, the Enforcement Directorate also had issued a blue corner notice against him.
In October 2013, the then spokesperson of BJP ,Nirmala Sitaraman called lalit Modi a 'Fugitive', and said BJP abides by law of the country. Any tweet of lalit Modi stating his connection with BJP is counterfeit. An year later, foreign affairs minister Sushma swaraj is doing all favors to a 'Fugitive' on so called 'humanitarian grounds'. Saying that she did whatever she could do for any other Indian, swaraj shunned out from the whole issue. But BJP can't flick tail from the matter as opposition has strongly raised voice on standards of BJP government and Rahul Gandhi's statement of Suit Boot ki sarkar has become stronger in a way.
However on the contrary, BJP is constantly justifying the undue help to Lalit Modi by asking the Congress about Warren Anderson and Quattrocchi. But that's where party exposes its double standards. Giving a statement that Lalit Modi was only Fugitive for UPA Government, is ignoring the strong facts of the scam that Lalit Modi did. Is India a banana republic, where Action of impropriety isn't a big deal? For any government, it implicates the same approach.
According to media reports that emerged so far, Swaraj's daughter was a lawyer for Lalit Modi and the British MP Keith Vaz who was mediating for him reportedly helped secure an admission for a Swaraj relative at Sussex. More over, by Lalit Modi's own tweets, he is close to some BJP leaders.
Swaraj's action has punched a big hole in the BJP government's claims of corruption or scam free governance. And congress is demanding that Sushma Swaraj should immediately be expelled from the cabinet on basis of abuse of discretionary powers and exploiting conflict of interests.
It is the fact that 10 years of UPA government has filled up Country's lap with number of scams and loss. And Prime Minister Modi's drive to Rebuild India's image as a powerful country is appreciable. But these actions taken by cabinet ministers will put on an iota of doubt on all such efforts. Rahul Gandhi has put PM under palisade of this matter stating PMO's interference in all ministries.
It is however, really difficult for a layman, to understand all this. But as per media reports such actions of favoritism harm conviction of a common man in his government. Politics and business have played their role over each other at times, and they are intact at their places. And Modi's help is another imputation on Modi Government and this time it is going to be very hard to come out of this.
By: Ruman Hashmi (Chief Editor-Newsleaks)


text:
Jackson® Wildcat™ Safety Goggles; Polycarbonate, Clear, Black
Item #: 901-422563
Model #: 20525
Brand: Jackson Safety
Not yet rated.Be the first to Write a Review
$14.99 Each
Product Highlights
- Brand: Jackson Safety
Jackson® Wildcat™ Safety goggles in clear/black color are lightweight and comfortable for any work environment. Jackson® Wildcat™ Safety goggles in clear/black color feature adjustable side vent for ensuring greater air flow. Goggles offer high visual clarity and are suitable naturally to the contour of your face in order to block out air borne contaminants.
- 1 Each
- Black
- Extreme heat resistance - will not melt, drip or ignite at 350°F for five minutes
- Adjustable side vent to ensure increased air flow
- Fits naturally to the contour of your face in order to block out air borne contaminants
- Meets ANSI Z87+ (high impact) and certified to the requirements of CSA Z94.3 standards
- Lightweight and comfortable with a pivot headband that allows optimal placement
|Brand : Jackson Safety
|Weight : 0.10 lbs. per Each
Customers also viewed
Customers also bought
No Review


text:
posted by David .
1.) A vertical spring with a spring constant of 450 N/m is mounted on the floor. From directly above the spring, which is unstrained, a 0.30 kg block is dropped from rest. It collides with and sticks to the spring, which is compressed 2.5 cm in bringing the block to a momentary halt. Air resistance neglible, from what height above the compressed string was the block dropped?
(Steps please?


text:
Is it too late to renegotiate after a deal has been agreed?
I am a first time buyer looking for a buy to let property. I found a property that I really like in the town centre (yield over 6%). However due to naivety on the advice of the estate agent put a bid at full asking price to take the property off the market (there was a real hard sell by the estate agent). The vendor has now accepted the offer. However I feel I offered about £5000 more than I should have by bidding at full asking (£125k). I am now wishing I had started with a lower offer and then worked up, I know its a lesson I'll learn from. Do I have any options for renegotiating after the deal has been agreed? There may be some unforeseen work in the kitchen area that would give grounds for renegotiation. I don't want to lose this property or aggravate the vendor so they pull out on me and I haven't instructed a solicitor as yet.
Asked on Jun 9 2013, House Prices in Guildford | Report content
Answers (2)
Sort by: Oldest | Recent | Highest rated
As you said you will learn from this experience. Whilst you don't want to lose the property, it's also clear that you don't want to pay the £125k agreed. I would go back to the agents now and try and renegotiate before you or the vendor has any associated costs. Yes you run the risk of the vendor saying no, but you have nothing to lose, and if the agent did "hard sell" then I guess he or she is confident they will find another buyer if a deal can't be struck with you. I think this is a case of honesty is the best policy, good luck and hope it works out for you and if it doesn't then it wasn't meant to be.Answered on Jun 10 2013, Report content
Darrins advice is spot onAnswered on Jun 13 2013, Report content
Didn't find what you were looking for? Ask a question
More House Prices in Guildford questions on AskMe
Asked on Apr 8 2014, House Prices in Croydon
3 answers - Answer this question
House Prices in Shirley, Croydon
Asked on Aug 15 2012, House Prices in Croydon
Be the first to answer this question
Do you know if there are any proposed building projects associated with Old Town CRO?
Asked on Jun 27 2012, House Prices in Croydon
2 answers - Answer this question
Property valuation in Sept 1997
Asked on Jan 22 2012, House Prices in Croydon
4 answers - Answer this question


text:
A recent poll asking readers to weigh in on Taylor Swift and Heidi Klum's cleavage may be a new low for the Detroit Free Press -- and yes, that pun is intended.
Last month, the newspaper published a poll online that gave readers the chance to vote "good" or "bad" on the cleavage of 12 celebrities. "When cleavage is good, it's very, very good. When it's bad, it's atrocious," the paper explained.
Georgea Kovanis, the Free Press writer who is credited for the poll, also offered "Dos and Don'ts of Cleavage" and listed the celebs on her good and bad lists in an accompanying column.
Those who bothered to vote were most impressed with Sofia Vergara (3,200 "good" votes) and least with Lena Dunham (2,514 "bad" votes), but plenty of readers had stronger opinions about the paper's judgement.
"Detroit Free Press, you've sunk to a new low in journalism. And there are a lot of lows to compare with," reader Marla Cone commented on the article. "Please keep your comments smart and civil. Unlike, of course, the poll itself," said Stephen Whitty. "This paper has finally jumped the shark," wrote Benny Leibhan.
After media blog JimRomenesko.com linked to the story Tuesday, the outrage spilled onto Twitter.
Yikes, @freep, did you accidentally publish an article from 1950?http://t.co/Q7LPZybXB2 @HannaRosin @NoreenMalone @DoubleXMag @meganharris13
— June Thomas (@junethomas) February 4, 2014
I can't express how utterly flabbergasted I am at this in 120 characters. http://t.co/ywiCefI5C2 Journalist integrity is dead at the @freep.
— Benny Vasquez (@bennyvasquez) February 4, 2014
OMG -- RT Judging women's breasts not "news".MT @HiddenBrain: Shouldn't @freep change its twitter handle 2 @sexist? http://t.co/nwqqMO34pt
— Ellen Comisar (@elesscom) February 4, 2014
I'll say it first: The Detroit Free Press must be run by boobs. "Vote: Good cleavage or bad cleavage" http://t.co/4hJpuxGQfo via @freep
— Kevin McDermott (@kevinmcdermott) February 4, 2014
Free Press Managing Editors Julie Topping and Nancy Andrews told The Huffington Post that the poll, which initially only had a line of two of text leading into the voting, went along with Kovanis' column leading into the Grammys.
"Online, we asked people to vote on which celebrities showed cleavage in the best way," they said in an email. "Unfortunately, the language of the poll lost the context of the column. We have since added more context."
The poll now includes part of Kovanis' column, which weighs in on the celebrity cleavage "trend." As red carpet style filters into the mainstream, "we're going to be seeing even more cleavage, coping with a cleavage explosion at work, at social events, even in front of our own mirrors," she wrote. "Cleavage can make women feel feminine, which often makes them feel pretty and sexy and confident."
But certain parts of Kovanis' column might not make those who took issue with the poll any happier, like when she writes about how women use their breasts.
"No matter how feminist their leanings, most women get a certain charge, a certain power from using their femininity to catch a man's eye or, in some cases, using it as a form of Kryptonite," Kovanis said.
"Cleavage also can distract from anything its wearer is trying to say, any point she wants to make," she wrote. "Instead of being seen as someone with a valid opinion, she runs the risk of being seen as someone with nothing to offer but breasts. Because that's where all eyes will be."
Though the few readers who responded to the poll itself may not have thought so, both Vergara and Dunham are winners in our book for loving their bodies and not apologizing for it.


text: 6 Blazers for All Occasions Facebook Pinterest Twitter Print Mail Save Left Arrow Right Arrow Photo by J. Crew Left Arrow Right Arrow 2 of 7 Grid View See All For Everyday Wear Top just about any outfit―from dresses to jeans to skirts―with this silk hip-length Zoey Blazer by J. Crew. Available in slate and black (shown). To buy: $145, jcrew.com. Start


text:
Although it means drastic steps, and without counting the cost, King's College now webcasts its services.
H/t Alberto Gonzales
Kathleen Pluth, director of sacred choral music at St. Joseph Academy, S.T.D. (cand.) student at the Angelicum in Rome, hymn writer and translator | archive
Fr. Christopher Smith, S.T.D., pastor of Prince of Peace Catholic Church in Taylors, SC | archive
Mary Ann Carr Wilson, music director at St. Anne Church in San Diego, home of the summer Chant Camp.
Adam Wood, church musician and writer at Music for Sunday.
e-mail: firstname.lastname@example.com


text:
DAISYOnlineService v0.1This service implements the DAISY Online Delivery protocol as specified in the Technical Recommendation approved in May 29, 2010.
Required operations
Optional operations
This is a demo service hosted by Kolibre and free to use for testing and demonstration purposes.
The service is pre-configured with five user accounts each containing three sample content. See list below for usernames and passwords
If a user tries to return a content, the service will respond with a successful response, but the content will appear in the list of new content when the list is requested.
The service is also pre-configured to reset itself to its default state every day at 0:00 CET