text:
Just a Number
A/N: Apparently I get really ambitious at this time of year. I just wake up one day and I think I can totally re-write a show with one of the main protagonists as an angel. Or that I can take away the coach-gymnast factor and still maintain the things we love best about Sasha/Payson shipping.
Disclaimer: I don't own MIOBI.
Summary: What if Sasha never came to The Rock? He was never her coach, and she was never his gymnast. Now all that stands between them is the age gap . . . and the Atlantic Ocean. Not to mention his reputation, the competition, and the fact that everyone seems to have an opinion on how she should feel about him and nobody thinks he's right for her. Nothing between them but age, and well . . . it's just a number anyway, right?
Just a Number – Have We Met?
World Gymnastics Championship, 2011 – Tokyo, Japan.
October 2011.
The four eighteen-year-old girls had hidden themselves in a corner of the stadium as they spied on the competition. Well, sort of. Technically it wasn't their competition they were spying on, even though the British Women's Gymnastics Team was practicing in the same area. None of them really cared that much about the British gymnastics team, except for one particular member who was currently accelerating towards the vault to a Yurchenko with two and a half twists: his easier vault.
"He's incredible," Payson Keeler awed, releasing the breath she had been holding until he landed. "I can't even think of the words to describe him," she sighed, causing her three friends to giggle at her uncharacteristic behaviour.
"Do you think he's going to do his signature vault?" Emily Kmetko asked eagerly, leaning forward in her seat. "He hasn't performed it since Athens."
"I heard they finally named it after him in the latest code of points," Lauren Tanner imparted in a covert way. "They gave it an 11.5 start value."
They all knew that she was exaggerating, but none of them corrected her. It was impossible to have a start value higher than ten, even if the five-time Wold Champion's vault deserved such a ranking. His vault was at least as difficult as Xio Jun Feng's, if not harder because of the transition between skills. The vault could barely even be classified into a particular form, falling within at least two of the vault groups.
The four of them watched in anticipation as once more he raced towards the vault at great speed. With a fluidity that even the most artistic gymnast would envy, he transitioned into a handspring, his hands hitting the floor and then the vault a few moments later. From the vault he pushed off into a double-salto in piked position, his aerodynamic form cutting through the air, and landed surely on his feet without even a hop in any direction.
Payson jumped to her feet as he raised his arms in a salute and clapped her hands wildly. "Go, Sasha!" she cheered loudly, lost in the moment. She gasped as she remembered their position, clamping her hands to her mouth in embarrassment.
The male gymnast looked their way, the sudden cheer certainly getting the twenty-six year old's attention. His grey-blue eyes flicked over the four of them, his frown slowly turning to recognition as he took in the four girls clad in their red, white, and blue Team USA tracksuits.
"You've done it now, Payson," Kaylie Cruz admonished. "He's going to have us removed."
But he didn't, instead sending the four of them an amused smile and a cheeky wink at the loudest of his four American fans. Payson grinned stupidly at the semi-flirtatious action and ducked her head to try and hide her cheesy smile from her friends.
Kaylie rolled her eyes. "Geez, Pay, what's the big deal?" she asked tiredly, seeming to lack some of the enthusiasm of her three fellow gymnasts. "It's only Sasha Belov."
Lauren, Payson, and Emily all gasped together in exaggerated shock, although genuinely appalled by Kaylie's blasé attitude to the Sasha Belov, one of the most decorated athletes in their sport since Nikolai Andrianov.
"Only Sasha Belov," Payson began on his behalf, keeping her voice to a low hiss so as not to engender their idol's attention once again. "He's only one of the youngest male gymnast ever to win all-around at the Olympics. He only held the world title for five years. He's only the only male gymnast to have won an Olympic gold medal in all six events. And he's only the favourite for this Worlds despite having been out of competition for nearly two years."
"And what am I, chopped liver?" interrupted a cordial voice touched by a southern twang. They all turned in his direction, Kaylie suddenly beaming with happiness at the appearance of her favourite Olympian.
"Austin, what are you doing here?" she asked warmly, gesturing for him come sit beside her. "I thought you were staying outside."
"I was," Austin Tucker responded, "but you guys were taking forever in here. What's taking you so long?"
"Payson's got a gymnastics crush on Sasha Belov," Kaylie responded snidely. "She won't let us leave," she added, even though she was the only one even willing to consider leaving before Sasha did.
Austin shrugged at her response. "Who doesn't?" he asked her idly as Lauren and Emily hummed in agreement. "I'm a guy and even I think he's a gymnastics god."
"That's not the point," Kaylie huffed, crossing her arms.
"He's looking our way again," Lauren said suddenly, ignoring Kaylie's sulky looks. She let out an excited 'eep' and quickly turned her head away, playing it cool as the renowned gymnast glanced in their direction. "Quick, Austin, go away before he thinks we're with you."
For a moment he almost thought that Lauren was trying to protect him (because while it was improper for the girls to be watching the practice of a low-ranking competitor, it was considered cheating for Austin to be watching the practice of the only thing standing between him and gold). Instead she was protecting herself. Her tone (and the lack of protest by her fellow gymnasts) suggested they were embarrassed of him and wanted him gone before Sasha got the wrong idea and thought they were friends or something.
But it was already too late, and Sasha was already jogging towards him, with no visible trace of the knee injury that had kept him out of competition after his fall at Beijing. The operation, as far as they could surmise, had been a complete success and he looked as good as ever (at least by the girls' appraisal) and only a little bit older.
"So these are your spies, Puiule," Sasha surmised by way of greeting, smiling at Austin's suddenly antagonized look. It had been a running joke in Beijing among the male gymnasts to give Austin childish pet names due to his diminutive age at the time. Three years on, Sasha (and pretty much every other gymnast he had competed against in Beijing) still hadn't let it go.
"Spies," Austin scoffed. "I like to think of them as my own foxy, super-talented, covert angels, only with better hair and no actual combat skills.
"Theyz ma gurls," he added with a flick of his wrist, earning himself four out of five eye rolls.
"We weren't spying for him, Mr. Belov," Emily assured him quickly with an imploring look. "We just wanted to see if you were still as good as everyone says."
"Sasha or Alexandru," he replied, a grimace flicking briefly across his face. "Never Mr. Belov.
"So what verdict did you reach?" he added, the question almost unnecessary.
"Better than before?" she gave as her questioning reply, shrugging her shoulders and giving an easy smile that made her look endearing enough to get away with almost any crime. Sasha gave a light laugh, shaking his head but not admonishing her blatantly toadying response.
"Have we met before?" he asked, frowning a little in concentration. "You all seem vaguely familiar, or perhaps it's simply your esteemed company," he suggested with a sarcastic wave in Austin's direction.
"I'm Kaylie Cruz," Kaylie answered quickly, stepping to her feet and offering her hand.
Sasha nodded as he took it in his own. "Two-time national champion," he deduced appraisingly before moving to Lauren.
"Lauren Tanner," she said haughtily, announcing her own title, "bitch of the beam."
Sasha nodded but smiled tightly before offering his hand to the more shy burnet beside her. "Emily Kmetko," she told him, "the one from the Y."
"That's nothing to be ashamed of," he shrugged. "You should see where my father grew up in Romania. It doesn't mean you have any less right to be here, Emily."
Emily just nodded shyly, blushing at his easy dismissal of one of her deepest fears as Payson stood up beside her.
Payson offered her hand, and he took it gently, his calloused fingers brushing up against her own. "You're Payson Keeler," he said softly before she could offer it herself, her name sounding like honey from his lips. "The li'l engine that could," he endowed affectionately, smiling all the while.
It was almost like an electric current passing between them, and Payson understood that this was kindred spirit – someone who shared her very soul in a most profound way. Had they been coach and athlete, they would have been so in synch with one another that words would be unnecessary and she would sense his gaze upon her even if she couldn't see it. Instead they were fellow competitors, and so the sense was that of meeting an equal, or one's perfect match – the person who most complimented you in every feasible way.
"You . . . you know who I am?" she asked shyly, the smile on her own face irrepressible.
He laughed lightly, shaking his head in amusement. "Miss. Keeler, I consider myself a fan of yours," he told her charmingly, making her smile even brighter. "I didn't know how I was going to get back into gymnastics after my injury, but seeing you do the same was an inspiration. I figured if you could come back like you did at Worlds after a major back injury, then who was I to complain about my knee."
"Thank you," Payson responded, unable to find better words. She usually felt so uncomfortable talking about her injury, but Sasha made it seem like a badge of honour. She had recovered from the worst possible injury for any athlete, and come back to her sport as good as ever, even if it had been a difficult journey. Some skills had been lost due to lack of practice, but recoverable, but others were lost completely due to changes in her body. But she was determined to be the same gymnast she was before her injury at Nationals – thus Sasha's endearment. The Little Engine That Could was the perfect metaphor to describe Payson Keeler, even if the allusion was older than she was.
"I suppose you ladies should go before the women's coach catches sight of you," he cautioned, glancing in the direction of his female counterparts. "She'll think the defending champions are trying to steal some great training secret from her and let it go to her head.
"Don't inflict that on me," he begged jokingly. "Coach Frost," he addressed sarcastically, "is already near impossible to deal with. I'd hate her to think she had a reason to be so uppity."
The girls nodded in agreement, having seen enough of the British women's coach to share his sentiment. Elsabeth Frost was a pedantic woman with no sense of her own mediocrity. He was right to think she would take their presence as a compliment to herself.
"It was nice meeting you, Sasha," Kaylie spoke on their behalf, taking responsibility as team captain. The three other girls nodded in agreement.
"You too, ladies," he agreed. "I'll be rooting for you," he assured them with another friendly wink, waving them off as they went on their way. His eyes lingered upon one particular gymnast's receding figure as she left, watching the unconscious sway of her hips until he caught himself doing so.
The girls chatted eagerly as they left, commenting on how nice Sasha Belov was in person and how he was nothing like what the media made him out to be. He always seemed so standoffish when the cameras were on him, but he'd been nothing but a gentleman to the four of them and positively charming. They discussed how handsome he was, the light stubble making him look rugged and dangerous compared to the clean-shaven chiseled look he usually wore for competition. Even Kaylie agreed that he was remarkably handsome, while adding the caveat that she personally preferred burnets without specifically mentioning Austin.
"Who'd have thunk it," their male companion commented, shaking his head at the feminine display.
"What?" Payson asked, wondering why Austin had decided to interrupt their discussion.
"You, Keeler," Austin answered vaguely. She gave him a curious look and so he explained, "you've gone all girly over Sasha Belov."
"I have not," Payson protested, flushing red at the insinuation.
"You have," Austin disagreed, "but at least now I understand why none of the other guys ever appealed to you.
"You little Lolita, you," he teased, catching her head in brotherly headlock. "I'm assuming it's the older guy thing and not the accent."
"Oh my gosh, we like haven't said anything about his accent," Lauren said suddenly, forcing the conversation back to the vital topic and so leaving Austin's teasing forgotten. "How dreamy is that accent?"
With renewed vigour, all four girls engrossed themselves in conversation about Sasha's accent with particular attention paid to the words that gave away his true origins. Austin's comments (and his presence for the most part) were completely forgotten and rightly so. Because there was nothing to them – not yet anyway – and they were hardly worth attending to. Not when they had more important matters to discuss . . . like the exactly colour of Sasha Belov's eyes.
~ to be continued ~
Hmmm . . . interesting start, right? I'm hoping I'm not the only person who thinks so. I had the vague notion that things would be so much easier if they weren't coach and athlete, but would it really? It's still an age-inappropriate relationship, and now we have a bad boy reputation to contend with. And the fact that they're on opposing teams. And Marty and MJ. And probably Ellen Beals at some point (probably Elsabeth Frost too as the English equivalent of Beals), although she's always going to be a problem irrespective of age gaps and the like.
Let me know what you think.
Notes: I would have preferred to maintain the typical age gap between Sasha and Payson, but it just didn't seem all that feasible. For one thing, it would have meant Sasha going into his fourth Olympics, and I couldn't find a single gymnast who had medalled in more than three. Secondly, I thought the 11 year age gap might have been pushing things without The Rock to bring them together. Anyway, because of that, Sydney 2000 becomes Athens 2004, except for the knee injury, which moves to Beijing 2008 followed by a miraculous recovery. This Sasha is a bit more light-hearted I suppose than the series Sasha (at least when he first arrived at The Rock), largely because he hasn't had to face a lot of the experiences that the series Sasha did and he still has his gymnastics to get him through.
I'm going to be posting this on my livejournal account as well (same name - virgowriter - cos I'm unoriginal) mostly because I can insert pictures and stuff if I need to with livejournal entries. I'm gonna try and make the fanfiction version of the story the 'clean' version - no authors notes before or after, and the only additional info you get are notes and translations. We'll see how that works. I might throw in the occasional additional material in there too, depending on my mood.
Romanian Translations:
puiule: cub/baby.


text:
A topological algorithm for identification of structural domains of proteins
Identification of the structural domains of proteins is important for our understanding of the organizational principles and mechanisms of protein folding, and for insights into protein function and evolution. Algorithmic methods of dissecting protein of known structure into domains developed so far are based on an examination of multiple geometrical, physical and topological features. Successful as many of these approaches are, they employ a lot of heuristics, and it is not clear whether they illuminate any deep underlying principles of protein domain organization. Other well-performing domain dissection methods rely on comparative sequence analysis. These methods are applicable to sequences with known and unknown structure alike, and their success highlights a fundamental principle of protein modularity, but this does not directly improve our understanding of protein spatial structure.
We present a novel graph-theoretical algorithm for the identification of domains in proteins with known three-dimensional structure. We represent the protein structure as an undirected, unweighted and unlabeled graph whose nodes correspond to the secondary structure elements and edges represent physical proximity of at least one pair of alpha carbon atoms from two elements. Domains are identified as constrained partitions of the graph, corresponding to sets of vertices obtained by the maximization of the cycle distributions found in the graph. When a partition is found, the algorithm is iteratively applied to each of the resulting subgraphs. The decision to accept or reject a tentative cut position is based on a specific classifier. The algorithm is applied iteratively to each of the resulting subgraphs and terminates automatically if partitions are no longer accepted. The distribution of cycles is the only type of information on which the decision about protein dissection is based. Despite the barebone simplicity of the approach, our algorithm approaches the best heuristic algorithms in accuracy.
Our graph-theoretical algorithm uses only topological information present in the protein structure itself to find the domains and does not rely on any geometrical or physical information about protein molecule. Perhaps unexpectedly, these drastic constraints on resources, which result in a seemingly approximate description of protein structures and leave only a handful of parameters available for analysis, do not lead to any significant deterioration of algorithm accuracy. It appears that protein structures can be rigorously treated as topological rather than geometrical objects and that the majority of information about protein domains can be inferred from the coarse-grained measure of pairwise proximity between elements of secondary structure elements.
Investigation of the structural organization of proteins is important for our understanding of the mechanisms of protein folding and function, and for insights into protein evolution. Direct determination of protein structures
Several attempts have been made to identify the structural domains of proteins. The most straightforward approach is based on visual inspection of a structure by a human expert. However, this approach is difficult to formalize, and therefore it is not easily applicable for analysis of large data sets. Another approach is to employ comparative sequence analysis. This method benefits from the vast collection of sequences from diverse organisms and high sensitivity of database search and protein sequence alignment. The shortcomings of this method is that, first, it relies on sequence similarity and thus is not applicable when the homologous sequences are not known; second, that the problem of defining the exact borders of sequence domains is itself difficult
A distinct category of approaches comprises fully automated methods
In this article, we present a novel algorithm for the automated identification of domains in a protein of a known three-dimensional structure. Our approach is based on ideas from graph theory. First, we represent a protein as an undirected, unweighted and unlabeled graph, which we call a protein graph. The vertices of a protein graph represent secondary structure elements. Two vertices are connected by an edge if the spatial distance between the corresponding secondary structure elements is below a certain threshold, and every pair of consecutive elements is connected by an edge ('backbone connection') by definition. Second, we determine all cycles up to a predefined length within this graph. A constrained partitioning of the vertices of the graph in two subsets results in two different types of cycles, pure and mixed cycles. The 'pure' type contains vertices from only one partition, whereas the 'mixed' type contains vertices from both partitions. Hence, there are three disjoint subsets, one for 'mixed' and two for 'pure' cycles. We examine cycle distributions induced by removal of each backbone connection in turn, and select the constrained partitioning of vertices that mutually maximizes the cycle distributions, thereby defining a tentative cut position along the backbone of the protein. Third, the decision to accept or reject the tentative cut position is obtained by using a special classifier. If the tentative cut position is accepted, the protein graph is split, and the three-step procedure is repeated with each of the two resulting subgraphs. Our algorithm stops automatically if the tentative cut positions are no longer accepted, and it does not rely on prior information about the number of domains.
Traditionally, approaches involving graphs use the
The representation that we employ is simple, and it does not take into account a wealth of additional information available in the protein structure data files, such as position and interactions of amino acid side chains, interactions with ligand and solvent, inherent disorder, and so on. It was not an intention of this work to gain a few percentage points on the already quite high average accuracy enjoyed by the methods that use all this information. Rather, we were interested to see how far we can get in protein domain dissection if we applied a more rigorous algorithmic framework that relies on a topological point of view and requires only a small number of assumptions. Perhaps surprisingly, our algorithm's average performance was comparable with all but the most advanced methods of heuristic domain dissection. The implications of this high achievement of a simple approach to our understanding of protein domain organization are discussed at the end of this work.
Results and discussions
We selected 2781 proteins from the ASTRAL database
Parameter optimization
Algorithm 2 depends on the following parameters: The maximal cycle length
We next investigated the influence of the maximal cycle length
Figure 1
Histograms for the number of secondary structure elements (#
Histograms for the number of secondary structure elements (#
Most of our graphs have more than 30 nodes. The determination of the cycles in a graph is a NP-complete problem
Figure 2
Objective Function
Objective Function
Results for multi-domain proteins
We used the test set consisting of 1871 proteins, which contained one, two, three, or four domains (accounting for 74.9%, 18.8%, 5.4% and 0.9% of all proteins, respectively) and the optimized parameters of our algorithm found from the training set. To evaluate the performance of our algorithm, we applied the error measure suggested in Jones et al.
Table 1
Results for the test proteins
number of domains
number of proteins
number of overcuts
number of undercuts
correctly assigned domain number
correctly assigned proteins
Table 2
Comparison of our results with the results from DomainParser ([19] old version, [20] new version)
DomainParser (old version)
DomainParser (new version)
Figure 3
Top row: Results for two-domain proteins
Top row: Results for two-domain proteins. Left: Histogram of the differences from the predicted to the assigned cut position by SCOP. Right: Histogram of the overlap values. Bottom row: Results for three-domain proteins. Left: Histogram of the differences from the predicted to the assigned cut position by SCOP. Right: Histogram of the overlap values.
In Fig.
Figure 4
Normalized objective function
Normalized objective function
We also examined trends in structure and fold classes of the proteins that were accurately dissected by DomainICA and those that did not behave well. Among the 235 proteins with correctly assigned number of domains, 16% of the domains were from the SCOP class of all-alpha proteins, 20% from all-beta, 38% from alpha/beta, and 26% from alpha+beta classes. For the 90(25) proteins which were undercut (overcut), 36(24)% were from all-alpha class, 11(38)% from all-beta, 29(18)% from alpha/beta and 24(20)% from the alpha+beta class. The fractions of correctly partitioned two-domain proteins were 53% for proteins with at least one all-alpha domain, 71% for the proteins containing at least one all-beta domain, 74% for the protein with at least one alpha/beta domain and 70% for those with at least one alpha+beta domain. Apparently, proteins with domains from the all-alpha class are more prone to erroneous partitioning than proteins that contain at least one beta-sheet. One possible explanation for this may have to do with the mean number of contacts in which a secondary structure element participates. A helix has on average 1.9 contacts to other secondary sturture elements, whereas a strand has 2.5, not including self-contacts and multiple contacts between the same secondary structure elements (recall that our algorithm does not use this information; the trend, however, is the same if multiple contacts are also considered). Thus, all-alpha domains are less connected on average, and the number of cycles in these graphs is smaller compared to graphs that represent proteins with beta-sheets. The other factor may be the generally larger distance between packed helices than between strands in a beta-sheet, which makes Θ = 6.2
Proteins that include discontinuous domains, where one domain is inserted into another, pose additional problems for our algorithm, because, for example, a two-domain protein would need two cuts instead of one and an additional step of fragment merging. Many such two-domain proteins, however, can be partitioned "almost correctly" if one part of discontinuous fragment is much shorter than the other, and, hence, one correct cut prediction is sufficient to fulfill
We finish this results section by discussing several examples that illustrate the working mechanism of our algorithm. In the following, the left figure shows always the domains assigned by SCOP and the right figure shows the predicted domains by DomainICA. In Fig.
Figure 5
A two-domain protein 1H72 (d.14.1.5, d.58.26.1)
A two-domain protein 1H72 (d.14.1.5, d.58.26.1). Left: Domain assignment according to SCOP. 5:167 (red), 168:300 (blue). Right: Domain assignment from DomainICA. 5–300 (blue).
Figure 6
A two-domain protein 1CRK (a.83.1.1, d.128.1.2)
A two-domain protein 1CRK (a.83.1.1, d.128.1.2). Left: Domain assignment according to SCOP. 1:98 (red), 99:380 (blue). Right: Domain assignment from DomainICA. 1–80 (blue), 81–263 (red), 264–380 (green).
Figure 7
A three-domain protein 1HS6 (a.118.1.7, b.98.1.1, d.92.1.13)
A three-domain protein 1HS6 (a.118.1.7, b.98.1.1, d.92.1.13). Left: Domain assignment according to SCOP. 1:208 (red), 209:460 (blue), 461–610 (green). Right: Domain assignment from DomainICA. 1–154 (red), 155–290 (blue), 291–610 (green).
Figure 8
A three-domain protein 1GSO (b.84.2.1, c.30.1.1, d.142.1.2)
A three-domain protein 1GSO (b.84.2.1, c.30.1.1, d.142.1.2). Left: Domain assignment according to SCOP. 2–103 (red), 104–327 (blue), 328–426 (green). Right: Domain assignment from DomainICA. 2–115 (red), 116–192 (blue), 193–426 (green).
In this work, we presented a graph-theoretical approach for partitioning proteins into structural domains based on two main new ideas. First, we represent proteins as unweighted, undirected and unlabeled graphs whose vertices correspond to the elements of secondary structure, including loops. Second, we introduced the mutual maximization of cycle distributions found in the partitioned graph as an approximate measure of domain compactness.
Several other algorithms have been suggested for the problem to identify the domains of a protein automatically
In this work, we did not strive first of all to provide numerical results about the identification of structural domains with better accuracy than DomainParser or other recently available algorithms. If this was our main goal, the most sensible approach might be to gradually refine the heuristics of these already useful methods, gaining in accuracy a few percentage points at a time. We set a radically different goal, namely to cast parsing protein structures into domains as an problem of optimization of a partition function that emerges from an extremely simple topological representation of a protein and requires the knowledge of an extremely small number of parameters. If this approach failed completely, this would not be of any interest – if, however, it approached the best available approaches in prediction accuracy, this would beg the question of which properties of protein structures are important in domain recognition and, by extension, whether the simple model is telling us anything important about protein structure and function.
The main conclusion from our study is, indeed, that despite extreme paucity of information that is presented by undirected, unweighted, and unlabeled protein graphs, the performance of DomainICA is closely comparable to DomainParser in the case of one-domain and two-domain proteins, which account for more than 90% of all proteins in the ASTRAL database and for a substantial fraction of complete proteomes in many organisms, especially in prokaryotes. It appears that more detailed information about protein structure, such as analysis of interdomain interactions at the residue level or considerations of protein physics and geometry, do not add much structural signal to our coarse grained representation. A corollary of this may be that proteins might be more properly treated as topological rather than geometrical objects, as has been recently speculated (see
Representation of a protein
We use the secondary structure elements of a protein –
Algorithm 1
There are several ways to obtain the secondary structure elements of proteins. We use the assignment provided in a pdb file
All connections determined by Eq. 2 are treated in the same way, regardless of the physical nature of the interactions (e.g., ionic, van der Waals, or other). We call an unweighted, undirected and unlabeled graph obtained by algorithm 1 a protein graph and denote it by
Partitioning of a protein graph
Structural domains of a protein are thought to be
Algorithm 2
In Fig.
Figure 9
A protein graph is split in two parts for a given boundary index
A protein graph is split in two parts for a given boundary index
Decision function
The crucial step in our procedure is the decision to accept or reject the suggested cut position
of an ensemble of
Definition 1
The decision function given in definition 1 was found empirically. The logical decision function
The second argument evaluates the breakdown of the randomized objective function. Application of our algorithm to proteins consisting of one or more domains indicates that the set of parameters for {
Authors' contributions
AM and FES conceived the study, FES developed and implemented the algorithm and analyzed the data, AM and FES wrote the manuscript.
We would like to thank Mike Coleman, Earl Glynn, Galina Glazko and Daniel Thomasset for fruitful discussions.
The Impact of Structural Genomics: Expectations and Outcomes
The three-dimensional structure of an enzyme molecule
SCOP database in 2004: refinements integrate structure and sequence family data
InterPro, progress and status in 2005
Nucleation, rapid folding, and globular intrachain regions in proteins
Recognition of structural domains in globular proteins
The multiplicity of domains in proteins
Toward consistent assignment of structural domains in proteins
An efficient algorithm for large-scale detection of protein families
SnapDRAGON: a method to delineate protein structural domains from sequence data
Prediction of protein interdomain linker regions by a hidden Markov model
SCOP: a structural classification of proteins database for the investigation of sequences and structures
CATH-A Hierarchic Classification of Protein Domain Structures
Partitioning protein structures into domains: why is it so difficult?
Protein structural domain identification
Some generalized order-disorder transformations
Superparamagnetic Clustering of Data
Data Clustering using a model granular magnet
Protein domain decomposition using a graph-theoretic approach
Improving the performance of DomainParser for structural domain partition using neural network
The ASTRAL compendium in 2004
Cyclic Pattern Kernels for Predictive Graph Mining
Domain assignment for protein structures using a consensus approach: characterization and analysis
Symmetries in proteins: A knot theory approach
Protein Similarity from Knot Theory: Geometric Convolution and Line Weavings
Automatic classification of protein structure by using Gauss integrals
Algorithmic Computation of Knot Polynomials of Secondary Structure Elements of Proteins
The Protein Data Bank
Dictionary of protein secondary structure: pattern recognition of hydrogen bonded and geometrical features
Knowledge-based protein secondary structure assignment
Hierarchic organization of domains in globular proteins
Continuous and discontinuous domains: An algorithm for the automatic generation of reliable protein domain definitions

subset: commoncrawl


text:
Digital LA - Bitcoin: What You, Consumers, Merchants, Developers Need to Know
Digital LA
Wednesday, January 15, 2014 from 7:00 PM to 9:30 PM (PST)
Who's Going
San Francisco, California
London, United Kingdom
Florence, Italy
Event Details
Bitcoin has made headlines recently as a potential new worldwide virtual currency. Join us for a panel discussion on Bitcoin with industry experts. We'll discuss what Bitcoin is, how to buy, when to buy, how merchants can set up Bitcoin purchases, to advanced topics including developer tools, international effects, and additional resources for Bitcoin business / developers. We are teaming up with the Bitcoin Meetup group, with support from the Bitcoin Foundation.
Speakers include:
- Brock Pierce, Clearstone Global Gaming Fund, Managing Director, and Bitcoin entrepreneur / guru / speaker. Brock Pierce has raised >$200M for his companies. He sits on the boards of IMI Exchange, GoCoin, ExpressCoin, Robocoin China, Mastercoin Foundation, Xfire, and Playsino. He was recently named a Bitcoin guru by USA Today, is a member of BitAngles, and has spoken about Bitcoin at events from the Milken Global Conference to Inside Bitcoin. @brockpierce
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- Elizabeth Ploshay, Bitcoin Foundation, Board Member, and Bitcoin Magazine, manager of communications.
- Joseph Hsieh, Expresscoin, CMO. Joseph Hsieh is a growth hacker, entrepreneur and virtual currency specialist. His last venture backed startup, Sometrics, was an analytics and virtual currency platform for social games and was acquired by American Express in 2011. Joseph consults LA startups on user acquisition and marketing.
- Michael Terpin, BitAngels founder. BitAngels invests in Bitcoin related startups. Michael is also founder of Social Radius, a social media marketing consultancy which has helped dozens of Silicon Beach companies with their social media.
- Brian Klein, Bitcoin Foundation member and partner at Baker Marquart LLP. Brian has written and spoken extensively about Bitcoin, and he is the chair of the Bitcoin Foundation's legal advocacy committee. At Baker Marquart, he focuses on representing individuals and corporate clients. He teaches federal criminal practice at USC's law school.
- Moderator: Kevin Winston, Digital LA, Founder. @DigitalLA @kevinwinston. Kevin is founder of Digital LA, Silicon Beach Fest, and has previously worked at Fox, MySpace, Sony and McKinsey.
- 7-8p. Checkin and Networking. Snacks provided by Krave Jerky. Tweet to eat @kravejerky
- 8-9:30p Panel
PARKING: Street parking nearby.
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Digital LA is the largest digital networking group based in LA, reaching more than 80,000 professionals weekly via its newsletter and social media. Digital LA organizes weekly panels and mixers for digital professionals, including startup CEOs, VCs and investors, entertainment including Hollywood movie and TV studios, music labels, agencies, video games, VFX, social media, digital advertising, developers, designers, marketing and and more. Digital LA is the founder of Silicon Beach Fest, the original and largest multi-day startup / entertainment conference. Follow @DigitalLA. Digital LA has been recognized by Forbes and the LA Times as keeping LA's tech community together via its fun and friendly events.


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See you there;)
See you there;)
MDHDAVIS on Jul 7, 2014
i love this top! very cute!
Michelle Sandon on Jun 24, 2014
This top is gorgeous and the open back with big bow is SO FUN! The quality is amazing as well! Love this piece!
ohsewsweetkkbb on Jun 11, 2014
Adorable! Seller was really nice and quick answering questions too. My daughter loves it and can't wait to wear it on the 4th. Thank You!
My story...
I started this boutique kind of by accident...
My sister found out she was having a girl (my first niece!). While running errands one day, I stumbled across a ruffled diaper cover pattern on sale for $1.50! Of course I bought it, and made a couple for my niece. My sister told me about Etsy!
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I am so thankful for my amazing husband for putting up with all of my fabric and constant purring from my sewing machine. My daughter for being her cute little self, and modeling all of my dresses. And most of all, my dedicated customers! I couldn't have done this without you all!
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Donnalee Shewmake
Owner, Maker, Designer, Curator
My name is Donnalee. I am a mother of a beautiful 4 year little girl, and a wife to my amazing husband. I am able to stay at home with my daughter, by being a tattoo artist and now my boutiuqe! I absolutely love what I do and wouldnt change it for anything <3
Shop policies
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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
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Is there a way to delete conditions all at once or only individually?Asked by Chris on March 22, 2016 at 11:37 AM
i have a bunch of conditions that have errors that i want to delete is there away to do them all at once or do i need to do them individually ?
To my current knowledge within our newer conditions wizard it is only currently possible to delete your conditions one at a time using the edit then delete option.
We had this option in the older conditions wizard and it'll probably be added in a future update to the new conditions wizard at some point later on.


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Nonetheless, head coach Larry Shyatt remains optimistic headed into tonight's game.
"We are still a good team, in that we are a good team that's been tested by some weaker opponents. Arguably we are 14-1 with 3 more baskets. There's a lot of great teams in the country that can't make that statement," said head coach Larry Shyatt.
The Deacons lead the Tigers in the overall series played between these two teams 80-55, but Clemson holds the edge at home, 33-31. That mark a 19-15 record at Littlejohn Coliseum.
The last time these two teams squared off at Clemson, the Tigers came away with a 118-155 double overtime win.
"I was disappointed we weren't awarded two wins after that game," said Shyatt. "It was a real sensational last minute of shooting by Wake Forest, and I just remember how we felt after that."
Wake Forest enters tonight's game winners of 3 of their last 4 games, including impressive victories over Georgia Tech and Maryland.
The Demon Deacons are led in scoring by Josh Howard (18.1/game), who is likely the early season candidate for player of the year in the Atlantic Coast Conference. Howard has four straight 20-point games and is averaging 22 points in conference play.
Defensive specialist Olu Babalola will likely match up with Howard, despite being hampered with a sprained right ankle that also has a strained peroneal tendon.
Clemson continues to be led in scoring by Ed Scott (17.1/game), however the senior out of Eastover has struggled in each of his last four outings. The Terps' full-court press at the end of the first half on Saturday saw Scott commit a barrage of turnovers in a crucial 13-2 Maryland run.
Starting center Sharrod Ford leads the way on the boards with 7.6 rebounds per game. Ford ended a streak of 13 consecutive games of shooting 50% or better when he went 1-4 from the floor against Maryland.
Probable Starters Clemson (11-4, 1-4 ACC) G 14 Chey Christie (6-4, 178) G 10 Ed Scott (6-0, 175) C 05 Sharrod Ford (6-9, 220) F 12 Olu Babalola (6-6, 255) F 43 Chris Hobbs (6-7, 255) Wake Forest (13-2, 3-2, ACC) G 04 Taron Downey (6-2, 175) G 10 Jamaal Levy (6-9, 180) C 31 Eric Williams (6-9, 270) F 05 Josh Howard (6-6, 203) F 00 Chris Ellis (6-8, 250)Other Notes
The loss to Maryland marked the 4th consecutive game that Clemson has been involved in game decided by 5 points or less. The Tigers lost to North Carolina by 2, beat Virginia by 1, lost to Florida State by 1, and then lost to Maryland by 5.
Both Ed Scott and Ray Henderson will be playing in their 100th game at Clemson University tonight. Incidentally, Scott needs just 13 points to reach the 1000-point mark in the college career.
Wake Forest has dominated the overall series between these two schools in last 6 years, winning 12 out of the last 15 games played between the two schools. The Tigers' double overtime win last year marked their first victory in the last 8 attempts against the Deacons.


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Results 1 to 5 of 5
2008-03-24, 23:43 #1
- Join Date
- Feb 2001
- Location
- Yilgarn region of Toronto, Ontario
- Posts
- 5,453
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what (printer) port is that? (Word 2000)
Edited by HansV to remove <!t>[pre] / <!t>[/pre] tags from extremely long lines that caused horizontal scrolling
Form_Load in Module 2 will list all the ports:
Microsoft Document Imaging Writer Port:
COM1: COM2: COM3: COM4: FILE: LPT1: LPT2: LPT3: BAR:

EnumeratePrinters1 in Module 6 will list all the printers:
There are 4 local and connected printers.
8388608 Microsoft Office Document Image Writer,Microsoft Office Document Image Writer Driver, Microsoft Office Document Image Writer Microsoft Office Document Image Writer
8388608 Generic / Text Only,Generic / Text Only, Generic / Text Only Generic / Text Only
8388608 BBBHP Color LaserJet 2600n,HP Color LaserJet 2600n, BBBHP Color LaserJet 2600n BBBHP Color LaserJet 2600n
8388608 BBBHP LaserJet 4L,HP LaserJet 4L, BBBHP LaserJet 4L BBBHP LaserJet 4L
My little FilePrint macro will detect the printer chosen by the user:
Sub FilePrint()
With Application.Dialogs(wdDialogFilePrint)
If .Display = -1 Then
Debug.Print .printer
End If
End With
End Sub

I can't work out how to determine the port that is used by the printer.
I'd like to use the printer name from the FilePrint dialog box to determine the port (I think) and allow ONLY printing to a printer port. i.e. if the printer name maps to FILE: I would dis-allow printing.

Module Purpose
Module 1 Get names of all local printers
Module 2 List all available ports
Module 3 Checks Printer? But wont compile in Word VBA
Module 4 Get printer driver
Module 5 Get a list of all printers
Module 5 Get characteristics of all printers

In the attached document, I have placed a url to each modules origin at the head of each module
2008-03-25, 00:17 #2
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Re: what (printer) port is that? (Word 2000)
I have attached a modified version of Module3 that will run. You can use one of your other modules to provide the printer names for the modified CheckPrinter function.
MsgBox CheckPrinter("Microsoft Office Document Image Writer")
2008-03-25, 16:51 #3
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Re: what (printer) port is that? (Word 2000)
Thanks Hans,
I couldn't get your modified to work late last night, but this morning realised that the "printers" collection was adding stuff I didn't need:
' Prn.Add Printer(0) & " on " & Port(0)
Prn.Add Printer(0)

I am now getting stuff I can use, in the sense of avoiding FILE:
Microsoft Office Document Image Writer
Printer Port Name = Microsoft Document Imaging Writer Port:
Generic / Text Only
Printer Port Name = FILE:
BBBHP Color LaserJet 2600n
Printer Port Name = USB001
BBBHP LaserJet 4L
Printer Port Name = LPT1:
2008-03-26, 10:00 #4
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- Aug 2001
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- Jeddah, Saudi Arabia
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Re: what (printer) port is that? (Word 2000)
Try Googling prnadmin
I haven't had time to look at it myself but it seems to have a lot of functionality.
2008-03-26, 13:30 #5
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Re: what (printer) port is that? (Word 2000)
>Try Googling prnadmin
V. much obliged, sir!
Microsoft Told me about a registry branch with PORT data (HKLMSYSTEMCurrentControlSetControlPrint). Interesting.
technet Told me about various scripts e.g. prnmgr.vbs.
technet Told me more about "Inventorying the Current Printing Environment"
The attached Module.bas, derived closely from my C:WINDOWSsystem32prnmngr.vbs seems to do the trick, sufficient, at least, for what I want.
Look for and step through the little "TESTListPrinters"


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This luxury apartment is located in a tranquil. relaxing new development. Inlcudes swimming pool and landscaped gardens with far reaching sea views across the Famagusta valley, making this an ideal property for the family to relax and enjoy their holidays.2 bed apartment 1 double bed with on suite, 2 single bedsCable/Satellite TV and DVD playerSitting and dining
The resort is situated between the charming town of Paralimni and the village of Kapparis which marks the start of a series of beautiful coastal bays and covesKapparis is a quiet coastal village close to the towns of Protoras and Paralimni. It is peaceful and picturesque making it perfect for families and couples looking for a relaxing holiday but with all local amenities nearby.Paralimni is a market town with a historic square surrounded by traditional tavernas and restaurants. The twin-aisled vaulted church, dedicated to the Virgin Mary (Panagia) is decorated with unusual 18th century porcelain plates.The resort of Protoras is a lively resort with a water park which has crazy golf and amusements. The nearest beach, Fig Tree Bay, is so called as the beach is marked by a fig tree which, according to local legend was brought by invaders in the 17th century. There is a small island a few hundred meters from the beach to which many swimmers visit to explore.The fishing village and seaside resort of Ayia Napa, surrounded by fertile plantations of vegetables and fruit, incredible rock formations and windmills is now the major tourist resort in the region. The Ayia Napa Festival takes place every September and includes folk art exhibitions, musical and theatrical performances and displays of traditional Cypriot culture.At night Ayia Napa turns into one large dance floor. The bars in the centre of town open their doors and crowds spill out into the streets which are lined with restaurants and bars with an abundance of tourist and souvenir shops. Water World Waterpark, the largest water park in Europe and located close to Ayia Napa, is themed on Ancient Greece. The park features a lazy river, an activity pool, serpentine slides, and the labyrinth with 50 interactive play areas.Distance to amenities:Cafe/Bars/Restaurants - 1 kmNearest Beach - 1.5kmLarnaca Airport - 60kmWaterpark - 5km
Air condition in bedrooms and lounge, free of chargeFree parkingW/CFully equipped kitchen with electrical appliances making for easy self cateringBalcony/Terrace for outside dining or sunbathingCommunal swimming pool
* Indoor pool


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Simplicity 2914; ©1949; Girl's Pinafore and Panties: The back-buttoned pinafore has a dart-fitted bodice and side-cut skirt. The neckline, heart shaped in front, is deep and square in back. Ruffling trims the armhole edges. Corded piping finishes the front waistline seam and tie ends form a back bow. The leg and upper edge of the panties have casing for elastic. In Style I an eyelet embroidered ruffle tops a contrasting armhole ruffle. The ruffling is repeated on the lower edge of the skirt. In Style 2, lace trims the armhole and skirt ruffles. Pockets finish the skirt in Style 3 and rick rack is used as trimming. 10 pieces.
Links to reviews/blog postsEdit
- The Perfect Pattern Size 5
Add a photo to the gallery by clicking the "modify" button below.
[insert your username, and make sure your preferences allow for people to contact you via e-mail. Do not list your email address here!]


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In his post-session interview, bearded Nathan Martin looks stoned. He's not high on drugs or booze, his head is swimming in a "post-orgasmic bliss" as he calls it, adding, "It was very good." I believe him. Cock teased and brought to the brink of cumming several times, Nathan's got a lot of those feel-good chemicals coursing through his body.
I don't know who Nathan Martin is, this is his first appearance on Men on Edge, and I can't really find him anywhere else. But he's a sexy guy with a full beard and decent body, and he's swinging a big dick. He admits to being nervous before his session, but he's raring to go, so they tie him to a concrete pillar with bright red rope.
Nathan gets the full treatment from Sebastian Keys, although not as wickedly nasty as I've seen, but Nathan's a first-timer. Whether he's bound to the pillar or bed, Nathan is helpless to stop it. All he can do is yell, "I'm gonna cum," which only makes Sebastian stop. But this isn't only about Nathan's big cock, Sebastian tugs on his nuts and pulls on his nipples.
Nathan is tied to the bed and gets his dick serviced some more before Keys brings out the electric dildo and plunges Nathan's hole until he yells his head off. Finally, Sebastian puts Nathan out of his misery and he's got a special way of bringing this session to an end.


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Gov. Earl Ray Tomblin today announced the Education Efficiency Audit of West Virginia's Primary and Secondary Education System, conducted by Public Works LLC, resulted in more than 50 findings and recommendations which has the potential to improve student education.
"Eight months ago we sought out to discover how we could produce the best education for our students while receiving the highest return on our monetary investment," Gov. Tomblin said. "From the classroom to the state school board, this assessment shows us we have room for improvement. For the future of our children and our state, we must provide build a better learning and teaching environment in our classrooms and reduce administrative redundancies thereby directing financial savings back into the classroom. Every West Virginian concerned about our education system needs to take the opportunity to adequately review and discuss this report so we can begin to take advantage of its recommendations, and do so in a way that is right for West Virginia."
The auditors declared that West Virginia has one of the most highly regulated education systems in the country with many details linked to state code which results in little flexibility to modify policy and operations.
A copy of the full report can be found online on the governor's website directly at www.governor.wv.gov/initiatives/Pages/ImprovingWestVirginiasEducationSystem.aspx.
Public Works and its partner firm, MGT of America, conducted the audit and held community events as well as conducted surveys and other assessment methods to complete the audit of the education system on the state, regional and local levels.
Source: http://www.governor.wv.gov/newsroom/pressreleases/Pages/GovernorTomblinReleasesEducationEfficiencyAuditofWestVirginia%27sPrimaryandSecondaryEducationSystem.aspx


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Two titans have braced for Super Bowl weekend, but they're not competing for the NFL championship.
Instead, telecom giants AT&T and Verizon Wireless hope to survive the onslaught of phone calls, video messages, images and texts sent by thousands of fans crowding into Miami this Sunday.
AT&T has rolled in three mobile cell sites – used to relay cell phone signals between towers – to boost network capacity around Miami's Sun Life Stadium, and has another mobile cell site to cover the Fort Lauderdale hotels where the New Orleans Saints and Indianapolis Colts are staying. It has also increased the capacity of permanent cell sites around Miami, Fort Lauderdale and South Beach, in hopes of ensuring a "winning wireless experience," as an AT&T announcement puts it.
The telecom's 2G and 3G networks inside the stadium also received a special boost to their voice and data capacity, so that fans can keep distant friends and family updated.
"These are two of the biggest games of the year, and fans attending them want to be able to share the experience, as it's happening, with those back home, whether its through a voice call, text message, e-mail or picture and video messaging," said Rich Guidotti, vice president and general manager for AT&T Mobility & Consumer Markets in South Florida.
Verizon Wireless took a slightly different tack by focusing on more permanent upgrades for Miami's stadium, and for the wider 3G network in South Florida. Such a permanent system can supposedly handle twice the capacity of what mobile cell sites can support at special events.
"South Florida hosts many professional and college championships, big games and other major gatherings," said Pam Tope, president of the Verizon Wireless Florida region. "We made this investment to ensure that we always have a strong wireless network for visiting fans, journalists and local wireless users whenever there is a big event."
Verizon's new system for the stadium alone includes two ground-level base stations and many antenna arrays. The telecom has also enlisted the aid of Qualcomm to "optimize" its wireless network and deal with the hundreds of thousands of visitors expected to throng South Florida.
A Verizon representative declined to give out the exact numbers of calls or messages sent from past Super Bowl games, but told TechNewsDaily that the height of this Sunday's game may see millions of calls flood the Verizon Wireless statewide network in Florida.
Both AT&T and Verizon plan on continuing to invest millions in their South Florida network to improve coverage for future events. And Verizon plans to launch its next-gen 4G wireless network in 2010, so that next year's Super Bowl fans may have even faster voice and data transfer rates to share their game day experience
- Americans Are Info-Junkies
- How to Pick the Perfect Super Bowl TV
- U.S. Considers 'Internet Access for All'


text:
December 24, 2008 BY Aaron Hope
Although there's a wooden mark traditionally used to signify an inquiry propping them up, there is no question concerning the quality of Nike Air Max 90 runners. A sure thing for nearly two decades, we've seen countless colourways since the original Infrared release way back when.
If the 90's reputation weren't enough, Nike outfitted this pair with a lush perforated nubuck upper that screams quality. Throw in teal laces and other accents along with white heel-tabs and swooshes, and you've got a winner that hearkens back to the stellar series of running releases made just for JD Sports. These are available now at another British sneaker boutique, Size?
More photos below…
Filed under: Air Max 90 Europe Exclusive Just Released Running


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This sector covers two exceedingly worthy vivacity skills that more adults pay a "king's ransom" to reach. Adults pay a enthusiasm coach, enterprise coach, or publication plentiful of self-reformation books, to gain this psychological feature and advance these being skills. However, inwardly a Karate class, children can be molded toward a lifespan of accomplishment.
There are various types of success such as as: The strength of influence, mental, physical, spiritual, and textile happening. A small fry can get a spicy grasp of maximum of these types of successes, within the skeleton of his or her Karate social class. Each fashion of success is valuable in today's international.
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The weight of urging is erudite through with national skills, and offspring have to hard work equally in instruct to swot Karate. Children drudgery in pairs, or groups, to complete a single-handed end. Sometimes, the maximal superior adolescent will head a group, patch the Karate guru (sensei) observes or corrects at solid points.
However, the potent Karate apprentice can be discovered voluntarily serving others. This is one of copious practical intrinsic worth that family learn, which are valuable in their Karate training, and it applies to having force in beingness.
Mental happening can be dry or something as undemanding as basic cognitive process a new military arts technique. The small fry who learns to use his or her success, gained in a military subject field class, toward existence outside Karate training, has scholarly one of the largest curriculum a Karate don can snap.
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Spiritual natural event is really gone up to the parents, but abundant advanced Karate students revise to cerebrate. Meditation offers the resources to unambiguous your noesis and tincture on one distinct concept, object, or hypothesis. At the highly least, kids Karate students will swot up to be barely audible and know how to soundless their minds. This is a alleviation for parents who urgently entail some melodious case in the daytime.
Material success arrives once all of the remaining forms of happening labour in chord. A youth who has the tools for success, studious inside the Karate classroom, will be competent to utilize them toward school, college, and company. For utmost of us, success, in life, will not be attained without serviceable at it.
As a toddler attains a higher category inside his or her Karate class, the resources to front a teensy knot of Karate students is well-educated. To deepen this quality, we developed a "leadership troop." These children, on the activity team, have displayed direction and kindliness toward their fella Karate students.
However, all juvenile person within our North Providence kids Karate system of rules is schooled the merit of regulation as it applies to duration. There is a time to atomic number 82 and a instance to follow, but brood have to learn to presume for themselves for their own refuge and success. The power of individual pressure is a filling hamper on the minds of brood.
Proper doings is not ever common near children, but the toddler with imposing control skills will not track the negative walkway of force. Children have academic day by day concerns almost somebody pressure, drugs, and bullies. Karate training, and the leading skills that upshot from it, will carry your tike in the precise route.


text:
Well it has been a hell of a nine days since we left DC. For the two weeks prior to our Nov. 14 departure, we were in a haze of celebration of George's attainment of the Ph.D. (go husband!), fevered attempts to give away or sell 95% of our possessions, preparations for the move and for the road trip and lots of goodbyes. Now, my friends, we are nomads and living on the road!
I do the majority of my posting on Instagram and I attempt to post something every day. If you want to follow along, that's the medium where you'll get the most timely updates. But for those who can take theirs all at once: the first seven days.
We started the road trip, fittingly, in DC at the Renwick's Wonder exhibition – which is really freakin' rad and I'm a bit sad I'll never get to do an engagement session there. Ideal for my purposes. DC wedding photographers, you should be on that. Scope the rainbow:
Here's George offering David an unwrapped candy from his pocket.
Then we were on the road! David and I went down Route 11, which was a really great alternative to I-81. We ended up at Natural Bridge for what was probably the best day of the trip so far (sorry George!). Here's our historic hotel.
Natural Bridge! It really is very pretty and impressive.
AND THEN. Guys. We went to the Virginia Safari Park and it was *awesome*. I haven't laughed so hard in years. The animals will just barrage you and take all of your food and slobber all over you. An emu bit David! Here's a thousand pictures:
The llamas actually stand in front of your car to block your way while their buddies come in from the side, velociraptor style.
Then we hit up Foamhenge, which is a to-scale replica of Stonehenge, but in foam. It's getting on in years and a bit falling apart. You just park on the side of the road and walk up the hill to it. Very odd, very delightful!
The next day we drove down the Blue Ridge Parkway, doing a couple of small hikes and taking in some mountain air.
Linville Falls.
David splurged on the Grand Bohemian in Asheville. Asheville was a really great place, though we only got to walk around for a little bit. This hotel featured many antlers.
Here's my car experiencing the luxe life.
Then we toured the ridiculously large and fancy Biltmore. It was pricey, but David treated so who cares! You aren't allowed to take photos in the house (bummer), but the gardens and conservatory were fair game. They had some sick orchids. The whole house was decked out for Christmas, too, so it was very pretty and celebratory.
On the way from Asheville to Durham, we passed by this amazing sign.
Then we were in Durham with Dwight and Laurie! Dwight's friend Mika gave us a tour of Duke gardens and grounds. They were impressive.
I made David drop this pine cone a bunch of times to get the photo on the right.
And we saw the Nasher Museum, mostly for their (tiny) Ansel Adams exhibit, before we went to Bollocks BBQ and ate a metric ton of animal meat.
And David tried his first corn dog! Which is a side at Cookout.
He was unimpressed.
Following day, we just hit the road and stayed on it all the way down to Savannah. One can't, of course, drive past South of the Border without making fun of it and walking around.
The mosquito-riddled Santee Wildlife Refuge for a break-up-the-drive hike-turned-walk.
It was humid and nasty, but the mosquitoes were intense so I buttoned up.
Then we were in Savannah and picked up George for one day of us all hanging out. Unfortunately, I was getting sick at this point and cranky.
Though Savannah, of course, is very pretty.
We took a ghost tour, as one does. So many spooky stories, though of course I'm quite the skeptic.
And there was an amazing chocolate shop that looked like a tiny library. And the chocolates were mad tasty.
And, finally, Spanish moss.
That's it for Week One! We're already in day ten, so hopefully I'll get a new update out to you not too long from now. Keep up with Instagram and you'll get them in mostly real time.


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We have a special team-up between OSV and VGMO for the return of the podcast! (One that will likely continue moving forward.) To celebrate the Halloween season, I talk with their Oliver Jia and go into a complete nerd-out about all things Castlevania!
Special thanks to the VGMO crew for allowing me in on the podcast, and for their editing and mastering. You can check out the podcast notes here – VGMO Podcast Episode 6: Castlevania
You can also download the episode on their iTunes to listen on the go!
This past Monday was the 30th anniversary of Castlevania, a franchise that has been a cornerstone of video gaming for so many of us since our childhoods. On September 26th, 1986, Akumajo Dracula, or Demon Castle Dracula as it translates, was released for the Famicom Disk System in Japan, and the legendary feud between the Belmont clan and Count Dracula was born.
For me, Castlevania was not only an indulgence of my childhood love of vampire stories and movie monsters, but the music of the series became the baseline for which I rated so many game soundtracks through my life. Granted, I loved my Sonic the Hedgehog and Golden Axe as a Genesis kid, but the soundtracks from the Castlevania series stuck with me, and impacted a lot of how I perceived game music from childhood to adult hood. If I played it at 8 or 28, the series soundtracks are hands down my favorite of any game franchise.
Thus, in a bit of self-indulgence and as a tribute to 30 years of whip-cracking, wall meat-finding, vampire-slaying goodness, I've made a list of my favorite music from each game in Castlevania's lifetime; from the very first game all the way through to Lords of Shadow. (I'm omitting the Pachislot games as they're basically all re-used music and Castlevania: Lords of Shadow – Mirror of Fate since I never played it) Granted, OSV has covered the music of Castlevania several times before, but consider this a brief perusing through of the history of the franchise's music in all its iterations.
Be ye warned; here there be fangirling.
I've been using Reason ever since version 1.0 to make video game remixes. Reason has always been plagued with problems that its community has suffered through, accepting its flaws as features. I have always been a huge supporter of Reason but it's only until recently that I have grown to dislike Reason 6.5 due to certain features offered in the latest software. I'm talking about Rack Extensions, which is essentially Propellerhead's version of Plugins for Reason. It's such a gamble to allow plugins after releasing 6 versions of Reason that I feel it's only fair in critiquing them on their flaws surrounding Rack Extensions. For me, these flaws are so bad, I'd rather not use Rack Extensions. I'd like to go though some of these flaws, and offer some solutions to the problems in Rack Extensions.
More after the Jump.


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- Expanding debit card processing with the certification of Maestro® for the Middle East and Africa, complementing Planet Payment's support of VISA® Debit and Interac® in Canada
- Adding EMV support for online PIN entry for the Middle East and Africa, building on the Company's existing support of EMV for the Asia Pacific, Canada and Europe regions
- Signing agreements to integrate to the iPAY gateway a suite of enhanced, e-commerce, merchant fraud detection and prevention services.
Planet Payment, Inc. (USA: OTCQX: PLPM, UK: LSE:AIM: PPT and PPTR), a leading international payment and data processor, announced on November 9, 2010 its results for the three and nine month periods ended September 30, 2010. The third quarter of 2010 was another period of solid growth. Total revenue was up 32% to $15.5m (Q3'09: $11.7m), with multi-currency revenue up 47% to $12.5m (Q3'09: $8.5m). Gross profit for the period increased 22% to $5.0m (Q3'09: $4.1m). Adjusted EBITDA for the quarter increased to $0.4m from $0.2m in Q3'09. On a GAAP basis, net loss narrowed by 44% to ($0.4m) compared to the Q3'09 loss: ($0.8m), and also narrowed by 47% sequentially over the prior quarter (Q2'10 loss ($0.7m). See Table 1 for reconciliation of net loss to Adjusted EBITDA. For the nine month period ended September 30, 2010 ("YTD'10"), total revenue increased 31% to $43.2m (YTD'09: $33m), with multi-currency revenue up 51% to $34.1m (YTD'09: $22.6m). Gross profit for the period increased 18% to $13.7m (YTD'09: $11.6m). Adjusted EBITDA loss for the nine month period approached breakeven to ($0.04m) compared to a YTD'09 loss of ($0.04m). On a GAAP basis, net loss narrowed by 13% to ($2.7m) compared to a YTD'09 loss of ($3.1m). See Table 1 for reconciliation of net loss to Adjusted EBITDA. Planet Payment's revenue growth reflects an increase in transaction processing volumes primarily driven by increases in active merchant locations as well as improving economic conditions. The Company's 46 banking and processing customers have continued to roll out Planet Payment's solutions in sixteen countries as reflected by the addition of 3,800 active merchant locations since Q3'09, a 39% increase. During the third quarter of 2010, the Company began activating the merchant pipeline from newly signed acquirers in Canada, the United Arab Emirates, Philippines, Singapore, Brunei, Sri Lanka, the Maldives and South Africa, adding over 1,600 new merchant locations in these countries alone. "Our services help acquirers open new sales channels, merchants sell more goods and services and cardholders enjoy informed choice and transparency at the point-of-sale," commented Philip Beck, Chairman and CEO of Planet Payment Inc . "As a result of our efforts this year to roll out our innovative products to new customers and taking into account the customary fourth quarter seasonal uplift in our business, we look forward to increased revenue and gross profit, resulting in positive cash flows and adjusted EBITDA for the balance of 2010." During the third quarter, the Company continued to broaden and enhance its services, including:


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There aren't many states as good at wrestling as Pennsylvania, so winning a state championship there is a very big deal, especially as a sophomore. Rizzo could potentially win two more and have his choice of colleges to choose from. Surprisingly though, Rizzo prefers football.
"I just like football more," Rizzo told Scout.com "I haven't spoken to that many schools about wrestling. I get a few letters here and there, but not what you would think. When I went down to visit Pittsburgh I stopped and spoke with them a little bit, but nothing major. My whole family has played college football, so it's always been part of my life."
To be an elite level wrestler requires training outside the school and a lot of time outside of the regular season. Rizzo trains at two of the top mat clubs in the region and with two of the best wrestlers the state has produced.
"I train at Young Guns with Jody Strittmatter who wrestled at Iowa," Rizzo said. "I also go to Cold Steel MMA and roll with Carlton Haselrig (UPJ)."
Haselrig was a former six-time NCAA champion in D-I and D-II. He is the reason why D-II wrestlers are no longer allowed to compete for the D-I championships. Haselrig was also a former NFL All-Pro guard for the Pittsburgh Steelers and New York Jets.
The 6-foot-1, 235-pound, fullback has received interest from Georgia Tech, Pittsburgh, Penn State, Navy, Kentucky, Cincinnati, Ohio State and Maryland. He recently visited Penn State to see the Nittany Lions take on Michigan.
"I had a great time at Penn State," Rizzo said. "I have never seen anything like it. The atmosphere there was great. There must have been like 100 recruits there and I spoke with a lot of them. I talked to Coach (Tom) Bradley a little bit. It's nice that he is from Johnstown, so we can relate to each other."
Penn State used to always employ a fullback, but nowadays it is forgotten weapon at times. Rizzo still thinks he is of value to the Nittany Lions.
"They (Penn State) always have two-three fullbacks on scholarship," Rizzo stated. "The still use a fullback some and they will rotate two of them in a game."
Rizzo has also visited Pittsburgh a number of times, including the Pitt-Rutgers game. He is close with former Richland Ram and Pitt walk-on safety Marco Pecora.
"I have been to Pitt a lot and I really like it there," explained Rizzo. "It is a different type of an atmosphere than Penn State, but I like that with the city and everything. I know Marco Pecora pretty well from wrestling and he is friends with my brother.
"Coach (Greg) Gattuso has been to the school a few times and he told me they are going to be needing a fullback to eventually replace Hyno (Henry Hynoski)," Rizzo continued. "I sent them my mid-season highlights, so hopefully after their season they will take a look at it and let me know."
Rizzo has also been to Atlanta to visit Georgia Tech.
"I just sent Coach (Mike) Sewak two game films," Rizzo stated. "I went to camp there and it went really well. I also went to their spring game. Their offense is great for a fullback and I think it would be a good spot for me. We also visited Kentucky, Cincinnati and Ohio State on the drive back." Rizzo and his Richland teammates (5-4) play Central Cambria this Friday and the Rams need some outside help to make the playoffs.


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Quill Brand® One-Touch 3-Hole Punches; 20-Sheet
- Brand: Quill Brand
- Quill Plus: Yes
- Punches up to 20 sheets of 20-lb. paper
- Requires 50% less effort using one-touch technology
- Adjustable to 2- or 3-hole standard punch patterns
- Adjustable paper guide for control
- Metal base with aluminum body
- Easy-access clip tray
- Left punch and the center punch can be moved to adjust
Quill Brand One-Touch 3-Hole Punches Fit 20 Sheets of Paper
The Quill Brand one-touch 3-hole punch cuts through multiple sheets of paper with ease, producing two or three holes to suit standard ring-file configurations.
The Quill Brand one-touch 3-hole punch requires 50 percent less effort to use, reducing the risk of strain to co-workers' wrists. Its ability to penetrate up to 20 sheets of paper at a time speeds up the time it takes to prepare papers and documents for filing. The punch also features an adjustable paper guide to aid the accurate positioning of holes, preventing messy finishes and wasted paper.
Sturdy Construction
The hole punch is constructed with an aluminum body on a strong metal base. This sturdy construction makes it less likely to slip or flip over during use, allowing co-workers to operate the one-touch controls with confidence and speed.
Clip Tray
The hole punch's base features an easy-access clip tray that collects the circles of paper produced during each punching session, ensuring they reach the trash can instead of littering the office like confetti.
|Brand : Quill Brand
|Quill Plus : Yes
|Weight : 4.08 lbs. per Each


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Dapper Buster by DanSchoeningWatch
©2009-2017 DanSchoening
love what you do.
Love this, excellent work. By chance if I hooked you up with some pencils and possibly inks of my self, or a pic could I see myself looking this awesome?
Very Cool, man.
You know Dan, it's just one of those nights with that green floating blob
With linework and colours this high class, it's impossible to not like.
He reminds me of the flunky ghostbuster from the recent game
sure do fly.
Awesome. Ghostbusters is my all time favourite movie. I have the new video game of it. This looks great.
I like the way slimer looks.
Awesome! XD
Well Done!
yeah it,s nice i like your art style!!!!! awsome artwork!!!!
Dude, awesome!!!
Who ya gonne call? GHOSTBUSTERS!!!
God, your a cool artist
I am jealous of this pic.
HAHAHAHA Way to go incorporating yourself into that one, Dan-O!
Who ya gonna call? I can't wait for Ghostbusters 3!
Nice i had thought had drawn this completely when i first saw it, very different than kid's normal lines. great work and kudos to the both of you.
I've been recently thinking of Ghost Busters since the Nostalgia Critic (thaguywiththeglasses) reminded me of them. Awesome work!
Totally awesome! And I love the new avatar, too. Very dapper.
That expression is priceless - and so YOU sir Wonderful job on the lighting sir
awesome! Go get that pesky Slimer.
Its about time we saw you as a ghost buster!
Hah, oh that's wonderful!
Just another day at the office.
nice work!
Excellent Work!
Pink shirt is pink.
Bustin Makes Ya Feel Good!
that's dope!
that's dope!
You played their new game yet? It's a lot of fun
I still can't believe my luck.
Dang it, now I gotta go pick up the Ghostbusters game again!!
If there's something strange
In your neighborhood,
It would be advisable to consult an expert on the subject
In your neighborhood,
It would be advisable to consult an expert on the subject


text:
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Charlottetown police cruiser
©Ryan Ross/The Guardian
Police quickly find vehicle that left scene of accident on Passmore Street near University Avenue
A young man tried to escape responsibility for an accident this past week but police were too quick for him.
"Charlottetown Police Services received a complaint Wednesday at 12:28 p.m. regarding a motor vehicle collision on Passmore St., at University Ave., and that one of vehicles had left the collision scene," said a police report.
Police located the run vehicle shortly thereafter and charged the driver with several offences under the Highway Traffic Act, said the report.
The accused is a 19-year-old resident of the Charlottetown area.


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Welcome to Harvey's Home of the Original Seatbeltbag
3 Recommendations
hollyaguirre's recommendation
This is my third seatbelt bag and I couldnât be happier. It is quite the head turner and garners lots of compliments. Sturdy, practical yet oh-so-hip. After all, you donât want to look like a total nerd using the wireless connection in the lobby of the Chateau Marmont.
My Collections:
girlHenry's recommendation
Very sturdy. I guess if it wasn't you'd be wondering what car this crappy sealtbelt might be in. I love mine. I get a lot of compliments on it and now they have lots of different designs.
elisacamahort's recommendation
I just think these are a cool use of a familiar material. I don't think that these are really re-using materials at this point, but they are made in the USA, and they are of a nice, durable quality that discourages thinking of fashion as a throwaway concept.
My Collections:


text:
FREE SHIPPING on orders of $85 or more
An excerpt from www.HouseOfNames.com archives copyright © 2000 - 2017
Britons. It was a name for someone who lived in county of Angus.
Renison Early Origins
Angus (Gaelic: Aonghas), part of the Tayside region of northeastern Scotland, and present day Council Area of Angus, formerly known as Forfar or Forfarshire, where they held a family seat from early times and their first records appeared on the early census rolls taken by the early Kings of Britain to determine the rate of taxation of their subjects.
Renison Spelling Variations
Renison Spelling Variations
Spelling and translation were hardly exact sciences in Medieval Scotland. Sound, rather than any set of rules, was the basis for spellings, so one name was often spelled different ways even within a single document. Spelling variations are thus an extremely common occurrence in Medieval Scottish names. Renison has been spelled Rayney, Rainy, Rainey, Rainnie, Rennie, Renny and many more.
Renison Early History
Renison Early History
This web page shows only a small excerpt of our Renison research. Another 174 words (12 lines of text) are included under the topic Early Renison History in all our PDF Extended History products and printed products wherever possible.
Renison Early Notables (pre 1700)
Renison Early Notables (pre 1700)
More information is included under the topic Early Renison Notables in all our PDF Extended History products and printed products wherever possible.
Renison In Ireland
Renison In Ireland
Some of the Renison family moved to Ireland, but this topic is not covered in this excerpt. Another 124 words (9 lines of text) about their life in Ireland is included in all our PDF Extended History products and printed products wherever possible.
The Great Migration
The Great Migration
Such hard times forced many to leave their homeland in search of opportunity across the Atlantic. Many of these families settled along the east coast of North America in communities that would become the backbones of the young nations of the United States and Canada. The ancestors of many of these families have rediscovered their roots in the 20th century through the establishment of Clan societies and other patriotic Scottish organizations. Among them: Luke Rainy who settled in Virginia in 1679; Johnston Rainey who settled in Charles Town [Charleston], South Carolina in 1763; followed by James Rainey in 1767.
Contemporary Notables of the name Renison (post 1700)
Contemporary Notables of the name Renison (post 1700)
Renison Family Crest Products
Renison Family Crest Products
See Also
See Also
Other References
The Renison Family Crest was acquired from the Houseofnames.com archives. The Renison Family Crest was drawn according to heraldic standards based on published blazons. We generally include the oldest published family crest once associated with each surname.
This page was last modified on 21 January 2016 at 14:17.
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How to connect java and mysql?
How to connect java and mysql?
Use mysqlconnector jar and get the connection object
Main thing here is ""jdbc API "" ... Means java database connection application programme interface will acting as a establishing the connection b\w java pro and my SQL ..... Through java program we can access the database ... Main here jdbc drivers are used ......
Load drivers of my SQL into java program. Establish connection with data base
Also connect with http get Or post class
You can download the jdbc for my sql
We can connect java with mysql using jdbc
Use mysql-connector jar file
By Configuring with MySQL connector...get connection object
U should have drivers of mysql nd u need to estsblish a connection with database
Register To Attempt Complete Test
Create Account


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SIA RU000032, Smithsonian Institution Office of the Secretary, Correspondence, 1843-1887
- Creator:
- Smithsonian Institution Office of the Secretary
- Title:
- Correspondence, 1843-1887
- Data Source:
- Smithsonian Institution Archives
- Dates:
- 1865, 1865-1879, 1843-1887
- Summary:
- These secretarial records apparently were separated from the main series before the latter were bound.
- Topic:
- Museums--Administration
- Subjects:
- Henry, Joseph 1797-1878, Baird, Spencer Fullerton 1823-1887, Smithsonian Institution Administration
- Form/Genre:
- Manuscripts, Collection descriptions
- Local Number:
- Physical Description:
- 1 cu. ft. (2 document boxes)
Finding Aids to Official Records of the Smithsonian Institution Archives
Table of Contents
Record Unit 32
Smithsonian Institution. Office of the Secretary
Correspondence, 1843-1887
Collection Overview
|General Information About This Collection
|Smithsonian Institution Archives, Washington, D.C. Contact us at email@example.com.
|Smithsonian Institution. Office of the Secretary
|1 cu. ft. (2 document boxes)
|Record Unit 32
|Language of Materials:
Descriptive Entry
These secretarial records apparently were separated from the main series before the latter were bound.
Index Terms
This collection is indexed under the following access terms. These are links to collections with related topics, persons or places.
- Henry, Joseph, 1797-1878
- Baird, Spencer Fullerton, 1823-1887
- Smithsonian Institution -- Administration
Physical Characteristics of Materials in the Collection
Administrative Information
Preferred Citation
Smithsonian Institution Archives, Record Unit 32, Smithsonian Institution. Office of the Secretary, Correspondence
Container List
Box 1
Miscellaneous Correspondence, 1843-1880. Includes "Plan of [Robert] Kennicott's Exploration," 1865.
Alexander Dallas Bache Will and Fund, 1867-1868
Hawkins, B. Waterhouse, 1872-1875
Tyndall, J., 1872-1873
Miscellaneous 4: Reports on Building Stone for Smithsonian Institution, 1860s
Miscellaneous 37, 1871-1879
Box 2
Irby, J.R., 1873-1875
Jenney, W.P., 1874
MacGowan, D.J., 1865
Parry, C.C., 1871
Peale, Charles Willson, 1870-1876
- Finding Aid encoded on: 8-4-2016
- Contact us at firstname.lastname@example.com
Using the Archives' Collections
The Smithsonian Institution Archives welcomes personal and educational use of its collections unless otherwise noted. Please cite the Smithsonian Institution Archives as the source of the content, and when possible, link to the Smithsonian Archives website (siarchives.si.edu). Please let us know how you are using our collections for your projects. If you would like to see a collection in person, please note that most records and special collections are stored off-site, so researchers should contact the Reference Team at least one week prior to visiting to ensure that records can be made available in the Archives Reading Room. For information on commercial licensing or other uses, visit Rights and Reproductions. More information available within the Smithsonian's Terms of Use policy.
Note: In order to access PDFs, you must have the Adobe Acrobat Reader software which is available free of charge.


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Ouch! Katy Perry Gets A new Piercing!
Video Credit - Instagram @KatyPerry
Last time I did this I was on my own with a safety pin & cube of ice @ 13. This time I thought it was best I left it to the pro's. Sorry mom (again)
We Love It!!! I'll be honest though, are piercings coming back?? I feel like a lot of people have been getting them lately and I feel like for awhile people weren't getting them as much! Hmmm maybe I should pierce my nose!!


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Belkin 1.2m Lightning to USB Cable
Belkin 1.2m Lightning to USB Cable
Belkin's Metallic Lightning to USB Charge/Sync cable gives you the most convenient way to power and sync your iPhone, iPad and iPod touch with Lightning connector.
2.4 amp cable supports iPad as well as iPhone and iPod charging
Fast charging and data transfer
Slim over-mould allows for charging with most cases
High-quality aluminium finish in colours that match your iPhone 6s and iPhone 6s Plus
Compact and portable
Plugs into any USB 2.0 port to charge
Did You Notice?
The cable's compact design means you can take it anywhere.
Apple Recommends For
Quickly charging and syncing your iPhone, iPod or iPad with Lightning connector.
What's in the Box
Belkin Metallic 2.4-Amp Lightning to USB Charge/Sync Cable
Tech Specs
Cable type: Lightning to USB
Cable Length: 120 cm
Connections: Lightning
Height: 0.71 cm/0.28 in.
Length: 124.46 cm/49 in.
Width: 1.5 cm/0.6 in.
Weight: 29 g
Manufacturer Information
Part Number
Mfr. Part Number: F8J144ds04-C00
UPC or EAN No.: 745883709090
Non-Apple Branded products / Third Party Products
Note: Apple's One Year Limited Warranty does not apply to products that are not Apple-branded, even if packaged or sold with Apple products. Non-Apple branded products may have the benefit of a manufacturer's warranty provided by the product manufacturer - Please see your product box and literature for details. Rights under the product manufacturer's warranty are in addition, and not instead of, any rights that you have under consumer protection laws.
iPhone Models
- iPhone 5
- iPhone 5c
- iPhone 5s
- iPhone SE
- iPhone 6
- iPhone 6 Plus
- iPhone 6s
- iPhone 6s Plus
- iPhone 7
- iPhone 7 Plus
iPad Models
- iPad with Retina display
- iPad mini
- iPad mini 2
- iPad mini 3
- iPad mini 4
- iPad Air
- iPad Air 2
- iPad
- iPad Pro 9.7-inch
- iPad Pro 10.5-inch
- iPad Pro 12.9-inch (1st Generation)
- iPad Pro 12.9-inch (2nd Generation)
iPod Models
- iPod nano 7th Generation
- iPod touch 5th Generation 32GB/64GB
- iPod touch 5th Generation 16GB
Answers from the community
What is the warranty on this product
- Asked by caroline M from Falkirk
- on 22/10/2016
Answer now(What is the warranty on this product)No answers yet
Does this cable work with life proof cases? Thank you!
- Asked by Sara H from Auburn
- on 11/12/2015
Does this fit the iPhone 6 life proof nuud case?
- Asked by Maria M from Oakland
- on 29/11/2015
Answer now(Does this fit the iPhone 6 life proof nuud case?)No answers yet
- Native Union KEY Lightning-to-USB Cable : Available colours
- Belkin Mixit DuraTek Lightning to USB Cable (1.2 m) : Available colours