text:
Because a non-profit run by nuns and monks is not religious enough.
And, of course, the Church needs to be punished by Those of Name.
Mark Shea's Blog: So That No Thought of Mine, No Matter How Stupid, Should Ever Go Unpublished Again!
Because a non-profit run by nuns and monks is not religious enough.
And, of course, the Church needs to be punished by Those of Name.
Well the State now does define when life begins, when it ends, what marriage is.
Is it surprising that the State would now decide what is religion and what is not?
If 90 percent of all American Catholics overwhelmed the White House & Congress with phone calls & emails about the HHS mandate & funding for Planned Parenthood, they would have no option but to hear us & we'd see results. Unfortunately we Catholics in the USA are too infected with indifference & lukewarms.
Yes, but most American Catholics have fallen for the idea that they can be Catholic (adjective) Americans (noun, basis of the creed.)
And with the top 5% $$ of Catholics going to "Catholic" high schools where they receive a watered down faith and a contraceptive mindset (life is not worth living if you can't afford 12K for HS per year and 50K per year college).. and the other 95% of Catholic children only receiving an 8th! Grade! education in the faith…. I don't see a way out of this mess short term.
And I didn't even mention Clergy who are "afraid" to preach! in homilies!
They start with "Seperation of church and state" as the only true Holy Writ.
Then discover that the State is all and all is the State.
You can't be a Christian and an American. It's time for all Christians in the South to embrace Southern nationalism and reject the Americanism heresy.
It's time for all Christians in the South to embrace Southern nationalism
Well, the last iteration of "Southern Nationalism" was in defense of their preference for holding slaves. Plus, the Yankees now have nuclear weapons. You need to rethink this.
"Whatcha gonna do now, Leander? The feds have got the atom bomb!" – Maverick Louisiana Governor Earl Long to ultra-segregationist Leander Perez.
Kirt Higdon
No I think we Catholics (families & religious alike) in the USA should from more intentional communities outside cities where we can be a shining light upon a hill, following a model like that similar to that described in St Augustine's City of God & the Benedictine models of civilization. Not all faithful Catholics should do this, we'll need some to stay behind in the secularized cities to continue witnessing to Christ, but more intentional semi-self-sustaining Catholic communities outside cities should be built. (Like the Pilgrims & Puritans attempted but without the puritanical nonsense & Protestantism.) No, We don't need to associate with historical movements that supported civil warfare & human slavery like "Southern Nationalism".
I think of the Redskins story above, and can't help but think there's a trend here.
Except that Nuns and Monks are no longer running it. I'm not sure of the proportion, but the shows, producers, guests, etc. are rarely religious under the traditional vows. I don't watch or listen much, but as an example, Theresa Tomeo has a women's show and "Crossing the Goal" is a men's show, but I don't remember any Nuns or Monks. Perhaps if I audit the credits?
Not that I think Obamacare – which the Bishops supported since they wanted "Caesar" to do their job – is proper, but before breaking down a wall of separation, one ought to think about what it keeps out.
It's the job of bishops to provide health care? Who knew?
Please look at the credits at the end of the EWTN tv shows. The names of many religious brothers/sisters from Mother Angelica's Franciscan order will be shown in the credits running things like lighting, music, editing, sets, etc. Besides those, many priests (Frs. Pacwa- Jesuit, Triglio, O'Connor, Women of Grace one, Leo Clifford, Life on the Rock, ) have their own shows, are co-hosts, or are official show chaplains. In addition to the aired daily Masses & prayer services. Also, the lay Catholics running EWTN & being their own hosts are FAITHFUL Catholics. Should religious freedom just be limited to those who are under holy orders & consecrated vows? This right of free religious expression belongs to all Lay faithful as well. I stand with EWTN 110-percent! I don't want the government trampling on my religion.
And that Domican priest with glasses that has his own shows too. And I forgot the Deacons (like Dcn. Burke-Sivers from Oregon, Deacon Bill Steltemeier RIP who was a big chief EWTN officer, Deacon Alex Jones,). Deacons are also part of the Clergy who serve & work for EWTN. Yeah, I'm pretty sure EWTN & its mission is unmistakably religious in its legal contracts, nature & definitions (which should have automatically exempted them from that dumb Obama mandate from the get-go, but as usual liberal secularist activist judges largely run the show). The Constitution means next to nothing to our modern judges, sadly
at least the employees at EWTN would probably never use contraceptives or abortafacients, unlike employees at catholic universities or hospitals.
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These are pretty much a must have in every vehicle, it's defiantly an easier way of using your phone especially for GPS. This phone holder goes on the vent and it's very easy to push into place.
I really liked that this phone holder can be turned toward the drive side or the passenger side, it even can be turned for a side view.
This defiantly held my phone in place securely and I feel like it doesn't take up much room at all. I purchased this at a discount from Amazon and I'm very pleased with how well it works.
These reviews are the subjective opinions of ChickAdvisor members and not of ChickAdvisor Inc.


text:
home design. Sunday , November 20th , 2016 - 03:58:37 AM
Quote from Balmain Houses by Benn and Penna Architects :
This buildings have–been designed as two usefully separate pavilions. The bigger of this two pavilions contains livingroom as well as kitchen area, while the smaller has bedroom area. The recently built third pavilion (by Benn and Penna) accommodates a house office for an academic couple and a new awning connects all pavilions.
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In no way does Mogome claim ownership or responsibility for such items, and you should seek legal consent for any use of such materials from its owner.


text:
"Cops wanna knock me, D.A. wanna box me in. But somehow, I beat them charges like Rocky." - Jay-Z
Comment Spam
I hope this isn't too much of an inconvenience, and that you'll continue to comment with fervor.
(Ok, I'll admit, I just felt like using the word "fervor.")
6 comments:
Ok, looks like it worked.ReplyDelete
Although, that's not really a word, it's just a string of wavy letters. But whatever...
I have just started getting comment spam too. The odd thing is it's often on posts from 2004. I'm not sure why they think that will be visible at all. Even spamming my front page won't do much good unless their goal is to annoy me.ReplyDelete
What I don't understand is why they bother to make their comments look like "real" comments. If you really must spam me, just give me the link to your site. Do they think anyone is buying their charade? "Hey! Your blog is really cool. Funny and informative. Check our my blog when you get a chance! It's about low-interest home loans!"ReplyDelete
Curtis and Kim,ReplyDelete
I think you are not the target audience for comment spammers, Google is. They want search engines to find the comment and count it as a vote for their web site. (The more inbound links a site has, the more important Google thinks it is.) They are hoping to be as unobtrusive as possible, so you will not notice their comment and delete it. Thus they add comments to old posts where you might not see them and try to disguise them so a careless glance won't reveal them as spam. However, they pretty much have to include the search terms they're targeting, such as "low-interest home loans."
Ah. I guess that makes sense, oh wise one. But then I wonder why Blogger, which is owned by Google, set up this whole word verification thing.ReplyDelete
Ohhhhhh. I get it now. Don't mind me, takes me a few days sometimes.ReplyDelete


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text:
by Staff Writers
London (AFP) July 18, 2014
A Russian-made surface-to-air missile is the most likely cause of the suspected downing of a Malaysian airliner over Ukraine, analysts said on Friday, as claim and counter-claim swirled over who launched the weapon.
The truck-mounted "Buk" missile is capable of soaring to the height of a civilian airliner like Malaysian Airlines flight MH17, unlike lighter and more widely available shoulder-launched weapons, defence experts said.
Developed by the Soviet Union in the 1970s to shoot down aircraft, helicopters, cruise missiles and drones, it is still widely used by both the Ukrainian and Russian militaries.
But pro-Russian rebels in eastern Ukraine had just hours before the disaster announced that they had seized a number of Buks themselves.
"Who has shot it down? There is lots of evidence which shows these were pro-Russian separatists who have done that," Russian defence expert Igor Sutyagin of the Royal United Services Institute (RUSI) told AFP.
He cited a Twitter post on Thursday by separatists, later deleted, "when they reported that they had captured precisely that piece of hardware known as Buk".
The radar-guided missile can hit targets flying as high as 72,000 feet (22 kilometres) -- more than twice the 33,000 feet MH17 was cruising at -- and have a range of 32km, said IHS Jane's Missiles and Rockets editor Doug Richardson.
The missiles travel at three times the speed of sound and have an explosive warhead weighing 70 kilograms (154 pounds).
There are two variants: the Buk-M1 and Buk M2, codenamed by NATO as the SA-11 Gadfly and the SA-17 Grizzly.
The US envoy to the United Nations, Samantha Power, said on Friday it was likely that an SA-11 was responsible.
The missile is produced by the Russian arms firm Almaz-Antey, recently targeted by US sanctions, at a factory in Ulyanovsk.
- Warning of an airliner -
Richardson said the missile system has a "friend or foe" identification system, but could not distinguish a commercial airliner from another unknown plane.
"It would not give you a warning that you were tracking an airliner," he said.
Buk missiles are complicated to operate, requiring three lorry-sized vehicles -- a command post, a radar vehicle, and a launcher with the missiles -- which suggests that some expertise was needed to operate them, analysts said.
Ukraine pointed the finger at separatist rebels, who -- if responsible -- could have either captured the missile from Ukrainian forces or have had it supplied by Russia, experts say.
Two Ukrainian military planes were shot down in the weeks before the Malaysian Airlines jet crashed.
A message on the official Twitter account of rebel group the Donetsk People's Republic said on Thursday that insurgents had seized a series of Buk systems from a Ukrainian surface-to-air missile regiment.
Hours later, the group's "defence minster" Igor Strelkov on his local social networking page claimed to have downed what they said was a Ukrainian military transport plane. Both messages were later deleted.
Eliot Higgins, who posts detailed analyses of weapons in Syria and other conflicts on his Brown Moses blog, said he had traced a video of a Buk missile launcher about 12 kilometres (7.5 miles) from the crash site.
"The video was uploaded on July 17th. I've found no other versions of it, and it was on a new YouTube account which was deleted a few hours later," he said in an email to AFP.
The launcher was "heading out of an opposition-controlled town, close to the town centre, heading south, which is towards the Russian border", he said.
Jonathan Eyal, International Director at RUSI, said it was "impossible to believe" rebels could have hit their target without radar and other logistical support from the Russian military.
But Russia's defence ministry said Friday that a Ukrainian Buk radar was operating on the day of the crash.
Related Links
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All about missiles at SpaceWar.com
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text:
About the Book
"Street Photography" meets today's espresso bar culture. Lucious color and rich monochrome photographs reveal both the laid-back life and the hot pursuit of the deal humming through the steam-jet of the cappuccino machine.
Young and old, Bohemian and grey-flannel, artsy-type or cop on the beat: today's coffee houses offer something they all are seeking.
Features & Details
- Category Arts & Photography
Standard Landscape, 10×8 in, 25×20 cm
58 Pages
- Publish Date Mar 16, 2009
- Tags Street photography
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text:
I was reading the article below about the rape of men from Clipmarks which I hit from StumbleUpon button. I can't remember exactly how I got on the subject of this whole thing with my 8 year old Grandson, Lil'Bill. I think I groaned out load and he asked what was wrong. I then went on to tell him a bit piece of the article.
I believe I said that they were raping these men in order to make them do things, specifically becoming a "Suicide Bomber."
(I questioned the validity of the article, was it true? If the act itself of homosexuality as well as rape[not sure about rape?] is punishable by death, how is it possible for it to be condoned as a recruiting tool used in their making of suicidal bombers. It does make sense, though it's just rather contradictory, don't you know?)
He looked at me puzzled and wanted to know more. I then explained the motive as to why they would kill themselves because of this being a "Holy War," we are fighting and I then said that it was the Muslim or Islam belief that upon their death they would be greeted by "72 Virgins"(Houri) in a place of paradise and that they wanted to redeem themselves.
It was somehow astounding to me, lil Bill's next question; "What Holy War?" I tried to explain a few things and found myself telling him about the World Trade Center bombing. I was equally astounded that he had no clue about the WTC attack of 9/11, isn't ignorance a bit of bliss? Should I have eluded his questions and allowed him to sit in the splendor of his youth? Ah, the irony of it all...
I couldn't really surmise that he did not know about the devastation that happened on 9/11. At that very moment in time, it was my day off from my Cook I job in Prison. I was exhausted from going through Interferon & Ribiviron Treatment for Hep C. It took a very long time to build up my white blood cell counts which were depleted from this horrendous treatment. The whole affair may have been exacerbated by the fact that I was, along with one other Cook I, responsible for an entire population (985 of the meanest, nastiest, craziest women that the Commonwealth of Pa. has to offer)of inmate meals. Well, we served and cooked two of the three meals served daily at the Chow Hall. I mean, just think about this; There are a few things you do not F**k with and an inmates food is one of them. Yea, the heat is on baby and this is a whole other story in and of itself.
As I said, it was my day off but I had to get up, regardless, first for "Count" and then it was off to my mandatory Med Line. That was not my fav place to go at all. You stood in a line upwards of 50 + inmates patiently waiting your turn to get up to the med line window so you could receive your share of psych drugs or whatever. I had just returned, I believe from that, went back to my room and my celly says "Go to the Day Room, OMG, the WTC has been hit or something." I then watched in horror as the second plane hit. By the next few minutes, Bells were ringing and the echo throughout could be heard, "LOCK DOWN, return to your cells at once for COUNT." We spent the entire day on Lockdown because of the plane going down in Somerset, Pa., close to the State Correctional Facility at Somerset in Pa.
It was a rather harrowing time, that seemed to last forever, right along and the same amount of time it took to eat the dry/nasty bologna sandwiches we were given in lockdown. I do say the imagination has time to allow you a real good work-up of all the possibilities there are with the thought that our facility might be next.
So, in explanation of the WTC attack, I went straight to YouTube and clicked on the video below. I sat with my grandson, watching and pausing with every question throughout about half of the video. He is very inquisitive, I do say and does listen to me and my stories, poor child. But after a while, I do believe he got the jist of things and lost interest, you know the attention span wanes quickly at that age.
I however, continued to watch. This is a really noteworthy piece about "The Falling Man." It is worth watching and truly does explore the ins and outs, especially concerning the picture above and how it captivated a Nation as well as the feelings behind the picture.
Raping Men as a Terrorist Recruiting Tool
"Fancy that! Al Qaeda does not discriminate on the basis of gender or religion. They not only kill both men and women, Muslim and infidel alike, they also recruit men and women by raping them!
Pink News, which bills itself as "Europe's largest gay news," outlet has just confirmed that al-Qaeda uses male rape as a tactic to recruit male human bombers in Algeria.
We have just learned about a Sunni al-Qaeda related plot in Iraq in which terrorists raped eighty Muslim girls and women, then turned them over to Samira Jassim who patiently and persistently persuaded the rape victims, (many of whom had been targeted because they were depressed or mentally ill), to cleanse their shame by blowing themselves and other Muslims up. Twenty eight women did so."


text:
PC-KIMMO: A Two-level Processor for Morphological Analysis
What is PC-KIMMO?
PC-KIMMO is a new implementation for microcomputers of a program dubbed KIMMO after its inventor Kimmo Koskenniemi (see Koskenniemi 1983). It is of interest to computational linguists, descriptive linguists, and those developing natural language processing systems. The program is designed to generate (produce) and/or recognize (parse) words using a two-level model of word structure in which a word is represented as a correspondence between its lexical level form and its surface level form.
Work on PC-KIMMO began in 1985, following the specifications of the LISP implementation of Koskenniemi's model described in Karttunen 1983. The coding has been done in Microsoft C by David Smith and Stephen McConnel under the direction of Gary Simons and under the auspices of the Summer Institute of Linguistics. The aim was to develop a version of the two-level processor that would run on an IBM PC compatible computer and that would include an environment for testing and debugging a linguistic description. The PC-KIMMO program is actually a shell program that serves as an interactive user interface to the primitive PC-KIMMO functions. These functions are available as a C-language source code library that can be included in a program written by the user.
A PC-KIMMO description of a language consists of two files provided by the user:
- a rules file, which specifies the alphabet and the phonological (or spelling) rules, and
- a lexicon file, which lists lexical items (words and morphemes) and their glosses, and encodes morphotactic constraints.
The theoretical model of phonology embodied in PC-KIMMO is called two-level phonology. In the two-level approach, phonological alternations are treated as direct correspondences between the underlying (or lexical) representation of words and their realization on the surface level. For example, to account for the rules of English spelling, the surface form spies must be related to its lexical form `spy+s as follows (where ` indicates stress, + indicates a morpheme boundary, and 0 indicates a null element):
Lexical Representation: ` s p y + 0 s Surface Representation: 0 s p i 0 e s
Rules must be written to account for the special correspondences `:0, y:i, +:0, and 0:e. For example, the two-level rule for the y:i correspondence looks like this (somewhat simplified):
y:i => @:C___+:0
Notice that the environment of the rule is also specified as a string of two-level correspondences. Because two-level rules have access to both underlying and surface environments, interactions among rules can be handled without using sequential rule ordering. All of the rules in a two-level description are applied simultaneously, thus avoiding the creation of intermediate levels of derivation (an artifact of sequentially applied rules).
The two functional components of PC-KIMMO are the generator and the recognizer. The generator accepts as input a lexical form, applies the phonological rules, and returns the corresponding surface form. It does not use the lexicon. The recognizer accepts as input a surface form, applies the phonological rules, consults the lexicon, and returns the corresponding lexical form with its gloss. Figure 1 shows the main components of the PC-KIMMO system.
Figure 1: Main components of PC-KIMMO +-----------+ +-----------+ | RULES | | LEXICON | +----+------+ +------+----+ |-------+ +-------| | | v v Surface Form: +------------------+ Lexical Form: spies ------->| Recognizer |----> `spy+s +----+-------------+ [N(spy)+PLURAL] | v +------------------+ spies <-------| Generator |<----- `spy+s +------------------+
The rules and lexicon are implemented computationally using finite state machines. For example, the two-level rule shown above for the y:i correspondence must be translated into the following finite state table for PC-KIMMO to use:
|@ y + @ |C i 0 @ --+------- 1:|2 0 1 1 2:|2 3 2 1 3.|0 0 1 0
(Note: as of May 1991, there is a beta test vesion of a rule compiler available, called KGEN. See below for more information.)
Around the components of PC-KIMMO shown in figure 1 is an interactive shell program that serves as a user interface. When the PC-KIMMO shell is run, a command-line prompt appears on the screen. The user types in commands which PC-KIMMO executes. The shell is designed to provide an environment for developing, testing, and debugging two-level descriptions. Among the features available in the user shell are:
- on-line help;
- commands for loading the rules and lexicon files;
- ability to generate and recognize forms entered interactively from the keyboard;
- a mechanism for reading input forms from a test list on a disk file and comparing the output of the processor to the correct results supplied in the test list;
- provision for logging user sessions to disk files;
- a facility to trace execution of the processor in order to debug the rules and lexicon;
- other debugging facilities including the ability to turn off selected rules, show the internal representation of rules, and show the contents of selected parts of the lexicon; and
- a batch processing mode that allows the shell to read and execute commands from a disk file.
Because the PC-KIMMO user shell is intended to facilitate development of a description, its data-processing capabilities are limited. However, PC-KIMMO can also be put to practical use by those engaged in natural language processing. The primitive PC-KIMMO functions (including load rules, load lexicon, generate, recognize) are available as a source code library that can be included in another program. This means that the users can develop and debug a two-level description using the PC-KIMMO shell and then link PC-KIMMO's functions into their own programs.
Who is PC-KIMMO for?
Up until now, implementations of Koskeniemmi's two-level model have been available only on large computers housed at academic or industrial research centers. As an implementation of the two-level model, PC-KIMMO is important because it makes the two-level processor available to individuals using personal computers. Computational linguists can use PC-KIMMO to investigate for themselves the properties of the two-level processor. Theoretical linguists can explore the implications of two-level phonology, while descriptive linguists can use PC-KIMMO as a field tool for developing and testing their phonological and morphological descriptions. Teachers of courses on computational linguistics can use PC-KIMMO to demonstrate the two-level approach to morphological parsing. Finally, because the source code for the PC-KIMMO's generator and recognizer functions is made available, those developing natural language processing language processing applications (such as a syntactic parser) can use PC-KIMMO as a morphological front end to their own programs.
(Note: as of May 1991, a program called KTEXT is available. It uses the PC-KIMMO parser and processes text, producing a morphological parse of each word in the text. See below for more information.)
Versions available
PC-KIMMO will run on the following systems:
- Windows
- Macintosh
There are two versions of the PC-KIMMO release, one for IBM PC compatibles and one for the Macintosh. Each contains the executable PC-KIMMO program, examples of language descriptions, and the source code library for the primitive PC-KIMMO functions. The PC-KIMMO executable program and the source code library are copyrighted but are made freely available to the general public under the condition that they not be resold or used for commercial purposes.
It should be noted that the Macintosh version retains the DOS/UNIX command-line interface rather than using the graphical user interface one expects from Macintosh programs.
For those who wish to compile PC-KIMMO for their UNIX system, we will supply the full sources. But you must also obtain either the IBM PC version or the Macintosh version in order to get all the sample files.
The PC-KIMMO release contains the executable PC-KIMMO program, the function library, and examples of PC-KIMMO descriptions for various languages, including English, Finnish, Japanese, Hebrew, Kasem, Tagalog, and Turkish. These are not comprehensive linguistic descriptions, rather they cover only a selected set of data.
Versions of PC-KIMMO and KTEXT are now available for PC's running a 386 CPU (or higher). The main advantage of these 386 versions is that they will use all available extended/expanded memory and will also use virtual memory. This means that you can load a lexicon that is larger than 640K (such as Englex described below). These 386 versions support VCPI-compliant memory managers such as MS-DOS 5.0's EMM386 and Quarterdeck's QEMM386. They do not support Microsoft Windows.
Related software: KGEN, KTEXT, and Englex
KGEN, a rule compiler for PC-KIMMO, was written by Nathan Miles of Ohio State University.
KGEN takes a two-level rule like this:
y:i => @:C___+:0
and translates it into a finite state table like this:
@ y + @ C i 0 @ 1: 2 0 1 1 2: 2 3 2 1 3. 0 0 1 0
KGEN accepts as input a file of two-level rules and produces as output a file of state tables that is identical in format to PC-KIMMO's rules file. Anything that KGEN does not correctly handle can be easily fixed by hand in its output file. KGEN runs under MS-DOS, UNIX, and Macintosh.
KTEXT is a new text-processing application that uses the PC-KIMMO parser. It accepts as input a text in orthographic form, tokenizes it into words, strips off and saves punctuation, capitalization, white space, and formatting codes, parses each word, and outputs the result to a quasi-database file with a record for each word. Its output data structures are suitable for further processing by other programs, such as a text interlinearizer, a syntactic parser, or a machine translation system. KTEXT is available for MS-DOS, Macintosh, and UNIX.
Englex is a morphological parsing lexicon of English intended for use with PC-KIMMO and/or KTEXT. Its 20,000 entries consist of affixes, roots, and indivisible stems. Both inflectional and derivational morphology are analyzed. Englex will run under Unix, Macintosh, or MS-DOS (the files are plain ascii and are identical for all three versions). Because of memory requirements, to run Englex under MS-DOS you will need a 386 cpu and the new 386 versions of PC-KIMMO and KTEXT. These 386 versions will use all available extended/expanded memory and virtual memory. They support VCPI-compliant memory managers such as DOS 5.0's EMM386 and Quarterdeck's QEMM. They do not support (or need) Windows.
How to get PC-KIMMO
For those who would like to try out PC-KIMMO for free, it is available for download at PC-Parse download archives
KGEN is available for downloading:
- MS DOS version kgen02.zip 32KB
- Macintosh version kgen02.sea_hqx 82 KB
- KGen sources kgen02.zip 60KB
Englix is available for downloading.
Antworth, Evan L. 1990. PC-KIMMO: a two-level processor for morphological analysis. Occasional Publications in Academic Computing No. 16. Dallas, TX: Summer Institute of Linguistics. ISBN 0-88312-639-7, 273 pages, paperbound.
____. 1993. Glossing text with the PC-KIMMO morphological parser. Computers and the Humanities 26:475-484.
Karttunen, Lauri. 1983. KIMMO: a general morphological processor. Texas Linguistic Forum 22:163-186.
Koskenniemi, Kimmo. 1983. Two-level morphology: a general computational model for word-form recognition and production. Publication No. 11. University of Helsinki: Department of General Linguistics.
Miles, Nathan L. 1991. Automatic generation of two-level FSM tables. M.A. thesis, Ohio State University. [Description of the KGEN rule compiler.]
Sproat, Richard. 1991. Review of "PC-KIMMO: a two-level processor for morphological analysis" by Evan L. Antworth. Computational Linguistics 17.2:229-231.
Document date: 27-Mar-1996


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I've always had Corsairs, and put them into a few builds for friends as well, HX's I think, and they've all been solid.
Currently got an AX760 (?) I think, been a while since I had a tinker and it's silent until I game, then it spins quietly every few minutes for about 5 seconds.


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Even though the United States is still reeling from the shocking revelation regarding Beyonce's lip-synced rendition of the national anthem (Syncer-gate? The Beyonce Affair?) we think that it's time for the healing to begin. Which is why you should check out these videos of fun. playing at the Inaugural Ball.
Yes, believe it or not, some of the musical performances that took place during the inauguration ceremonies weren't total fiascos.
Check out clips of fun. performing "We Are Young" after the jump.
Latest posts by Dayton O'Connor (see all)
- Frank Ocean And Producer Malay Sued For Infringement Over 'Channel Orange' Track "Lost" - March 10, 2013
- The Maine Wrap Recording On Fourth Album - March 10, 2013
- STREAM: Justin Timberlake – "Suit & Tie" (Dillon Francis Remix) - March 8, 2013


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Tag: sausage
Oktoberfest News 2013read more No Comments
Landbrot Bakery & Bar in New York, NY
Landbrot Bakery and Bar has arrived on the Lower East Side and in the West Village! There's a lot to love about this chic restaurant: they're open late, it's spacious and of meticulous decor, you can sit outside, their list of cakes fills nearly 11" of menu, and their selection of German craft beers aligns perfectly with our taste.
Knicks and Brats
The Knicks are still going strong in the playoffs, and Andrew Carmellini's beer-braised Schaller & Weber Cheddar Brats are a great way to celebrate between dunks at Madison Square Garden. The arena's gastronomical renovation has been met with mostly rave reviews, but Carmellini's sausages are a standout, the Carmelo Anthony of concessions.
NEW from Schaller & Weber!
It's not often that Schaller & Weber adds new products to their lineup, but when they do, you can be sure that they've been carefully prepared according to the same high standards all their other recipes follow. More importantly, these taste really, really good. Check out what's now available in-store, online, and from your favorite retailers:
Frische Blutwurst
Here's a recipe for a hearty autumn salade chèvre with Frische Blutwurst (fried blood sausage), which was passed along to us by Renate Schaller. Adapted from Martha Stewart.


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Welcome to the ineedhits Search Engine Marketing blog, where we share the latest search engine and online marketing news, releases, industry trends and great DIY tips and advice.
Leading search engine marketing firm, ineedhits has joined the Search Engine Marketing Professional Organization (SEMPO) as a corporate contributor.
Perth, 11 May 2005. ineedhits has joined the global industry body SEMPO (Search Engine Marketing Professional Organization) to contribute to the education about search engine marketing and to promote the value of search marketing.
ineedhits CEO, Jackie Shervington explained, "We have been following SEMPO's activities since its beginnings in 2003. We are very impressed with the growing level of professionalism and information coming from the organisation and decided we wanted to be a part of it. As members, we have gained access to some excellent research which is proving invaluable in developing our future business plans."
ineedhits has played a significant role in educating over 500,000 customers and subscribers about search engine marketing over the last nine years. As such, it was a logical step for ineedhits to join SEMPO and join resources with other search engine marketing firms to promote awareness of search marketing.
Jackie Shervington added, "The ineedhits SEMPO membership has also been positively received by ineedhits staff who look forward to contributing to this forum!"
Keep up to date with the latest from our blogs.
Introducing Clicks4Sure – ineedhits' new 'Set and Forget' Paid Search Advertising Plans
Clicks4Sure, ineedhits new paid search advertising packages, enable small and medium size businesses to obtain qualified traffic for their website ...


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Let us help you to get up to speed with the new regulation through AAMC Training Group's Payment Systems Industry Regulatory Update – Ban on Excessive Surcharge course. Enrol now and get 20% off!
Read More
Legal Practitioners, Real Estate Agents and Accountants, why wait to be regulated? AAMC Training is offering 40% off on AAMC - Anti-Money Laundering and Counter Terrorism Financing Course.
Receive 10% off on our Australian Taxation Law Financial Advisers Course and check out our Business and Leadership Fundamentals CPD Packages for Professionals, Managers and aspiring Business Entrepreneurs.
Learn how to inspire employees, innovate and cultivate excellence at your workplace with AAMC Training's customised package of online courses especially created for professionals, supervisors, managers and aspiring business entrepreneurs.
For those seeking to complete the FNS40815 Certificate IV in Finance and Mortgage Broking, AAMC Training is offering a workshop to take place in Wolloongabba in August.
There's still time left to enrol! Take advantage of the 20% discount available on all our Online and RPL Qualification courses!
Get 20% off on all our online and RPL Qualifications Courses and 10% off on Taxation Law for Financial Advisers Course
AAMC Training offers a course on Taxation Law for Financial Advisers that can help meet your registration requirements for the Tax Practitioners Board (TPB). To sweeten the deal, we're giving you 10% off when you enrol now!
Take advantage of this opportunity now to expand your service offering or start a new career!
For those who are Financial Planners, Insurance Advisers and Finance Brokers, AAMC Training offers great topics that will help further your career.
USI Requirements for Students Undertaking Qualification Courses
Financial literacy is a topic that has always been close to AAMC Training's heart. We've trained thousands of professionals now working in the financial services industry - delivering advice and guidance to their clients.
To kick off the New Year, AAMC Training is offering some new courses and more great discounts!
AAMC Training would like to wish you a very Merry Christmas!
We've got special treats for you this Christmas season! Get 20% off on our selected Financial Planning and Finance Broking Courses and win Christmas Hamper by entering to our draw!
Great news for our members and check out how to develop your IT skills
ITAC Training Schedule to watch for in different locations across India.
AAMC Training Subsidiary Licensed by Regulator to Deliver Training in India
Find out how AAMC Training can help you in acquiring the right skills..
Check out our new offerings, tips and development updates
Important information on the USI for all students...
AAMC Training is happy to announce the winner of the $100 Gold Class Movie voucher is....
Efti Pavlou from Peoples Choice Credit Union, South Australia
We would like to Congratulate Efti and thank all those who took the time to enter the draw.
The correct answers to our Spring Carnival & the 'Race that stops the Nation' Quiz were;
1. What year and day was the first Melbourne Cup race run?
A. Thursday, 1861
2. How many 'grey' horses have won the Melbourne Cup?
A. Six (6)
3. In what year was 'Fashions in the field' first held?
4. What is the current Melbourne Cup time record and which horse won in that time?
A. 3:16.30 Kingston Rule (1993)
5. Name one multiple Melbourne Cup winner?
A. Archer / Peter Pan / Rain Lover / Think Big / Makybe Diva
6. Name the trainer who has the most Melbourne Cup wins.
A. Bart Cummings
7. Who was the first female jockey to ride in the Melbourne Cup?
A. Maree Lyndon
From 1 January 2015, AAMC Training Group as a Registered Training Organisation (RTO), will not be able to issue a certificate for a Nationally Recognised Training qualification unless the student has provided us with their USI.
For any of our Members currently studying a course that will roll over into 2015 or who will be enrolling onto a course in 2015, our system will prompt you to update your profile with your USI number or take you through the application for a USI process at the point of registration.
New regulations are being introduced that require you to have a USI which will contain all your nationally recognised training records and results from 1 January 2015 onwards.
When applying for a job or enrolling in further study, you will often need to provide your training records and results. One of the main benefits of the USI is that you will have easy access to your training and records and results throughout your life.
AAMC Trianing is in the final throes of working with the government departments to have our system linked in with the USI system as we are required to validate and report on each students USI and training.
It is free and easy for you to create your USI and the attached PDF (above) takes you through the process - just visit www.usi.gov.au
AAMC Training Group is Proud to be associated with the following organisations:
Subscribe for our Newsletter
We support Carbon Neutral with a pledge to plant a tree for each student undertaking
one of our certified Face 2 Face and Correspondence courses.
© Copyright 2017 aamctraining.edu.au. All Rights Reserved


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The original Universal Audio 1176LN designed by Bill Putnam was a major breakthrough in limiter technology-the first true peak limiter with all-transistor circuitry offering superior performance and a... Click To Read More About This Product
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Home > Services > Heart and Vascular Care > Conditions We Treat > Valve Disease > TAVR > Our Team
View Additional Section Content
Published on October 06, 2016
Our specialists include Kul Aggarwal, MD, Sudarshan Balla, MD, and Senthil Kumar, MD.
To see and evaluate valve function, cardiologists use specialized noninvasive cardiac imaging, including 3-D echocardiography (or "echo"), cardiac MRI and a 64-slice CT scanner.
Our specialists include Ajit Tharakan, MD, and Richard Schmaltz, MD.
Patients who require surgery are in good hands and undergo procedures in state-of-the-art facilities. University Hospital's hybrid operating room combines the functions of a catheterization lab with the surgical functions of an operating room, allowing surgeons and cardiologists to perform both open procedures and minimally invasive procedures (including TAVR) in the same room.
Our specialists include Arun Kumar, MD, and Richard Webel, MD.
Interventional cardiologists oversee medication management of diseases and perform non-surgical procedures such as cardiac catheterization, coronary angiography and coronary stenting.
To schedule an appointment, contact:
Valve Clinic Jessica Holt, RN Nurse Coordinator 573-884-0296
Learn more about TAVR by watching this American Heart Association video.
Marcella Haskamp, 92, of Jefferson City, Missouri, shares her heart valve replacement story.
Copyright ©2017 — Curators of the University of Missouri. All rights reserved. DMCA and other copyright information.
Equal Opportunity/Access/Affirmative Action/Pro Disabled & Veteran Employer. For website information contact the Office of Communications. Disclaimer


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When last seen in the operating room, Hugh Grant was fainting in the midst of ''Nine Months,'' playing a weak-kneed new father. In a change of pace, he now appears as a brilliant and dedicated young neurologist comfortable with emergency room jargon and unfazed by the sight of blood. So Mr. Grant takes some getting used to in ''Extreme Measures,'' Michael Apted's brooding new medical thriller. Here he is barking out orders, bustling with a solemn frenzy worthy of Monty Python and daring to use words like intubated with ease.
Fortunately, once the story's first medical crises have been dealt with, the film itself moves out of the danger zone. Mr. Grant becomes reasonably credible even when cruising the streets of New York on a motorcycle, and Mr. Apted directs this spooky tale with the same cool exoticism he gave ''Blink.'' Brisk and unsettling, ''Extreme Measures'' finds Dr. Guy Luthan (Mr. Grant) stumbling onto an ominous medical mystery. Homeless men have been turning up on his ward at Gramercy Hospital displaying bizarre symptoms and begging for help.
Mr. Grant soon shows himself able to hurtle through this intrigue without altogether losing his sardonic edge. Asked why a woman at the hospital is screaming, he mutters: ''I don't know. She probably just got a bill or something.'' And when the medical trail leads him to the morgue, he looks over the sinister fellow in charge and observes, ''You're quite a creepy person, aren't you?'' The screenplay by Tony Gilroy takes a generic crusading doctor and, with the help of Mr. Grant's unlikely presence, gives it some color it might not otherwise have.
The plot of ''Extreme Measures'' also thrives on the unexpected, like the sight of two naked men making a frantic escape from imprisonment and racing into the middle of Manhattan traffic. Scenes like that keep the film off balance, though the story becomes more ordinary as it goes along. Sure enough, Dr. Luthan has stumbled onto a sinister plot, and there are those around him who would like to see it covered up. Dr. Luthan's superior at the hospital even delivers this classic warning: ''Let me put it to you this way. I'm your boss and I'm telling you, Drop this thing!''
Will anyone worth his Gray's Anatomy expect Dr. Luthan to give up? He begins connecting a diabolical research project involving the homeless men with Dr. Lawrence Myrick (Gene Hackman), a Nobel-Prize-winning doctor with considerable power over Guy's career. Also mysteriously in cahoots are two hulking, menacingly quiet enforcers played sturdily by Bill Nunn and David Morse.Continue reading the main story
Dr. Myrick turns out to be the kind of doctor who cares as much about interior design as research. (His top-secret lair brings a hint of Architectural Digest to a hospital setting.) And ''Extreme Measures'' eventually goes off the track when it sends Guy squirreling into the bowels of Grand Central Terminal and begins to resemble far-fetched science fiction. But much of the way through, this film remains stylish and taut with a taste for macabre little surprises. A bit of business involving a paraplegic and a fly is nicely Hitchcockian. And it's fitting that when Guy is sabotaged and brought before a hospital disciplinary board, his chief interrogator is played by David Cronenberg, who showed his own weird flair for medical mystery in directing ''Dead Ringers.''
Mr. Hackman brings a cool proficiency to something other than a standard villainous role. Sarah Jessica Parker plays a friendly, dedicated nurse who shares Guy's alarm. John Toles-Bey does well as Bobby, a homeless man who has a teasing camaraderie with Guy. The film's New York street scenes have a lot more authenticity than they should, since much of ''Extreme Measures'' was shot in Toronto.
''Extreme Measures'' is rated R (Under 17 requires accompanying parent or adult guardian). It includes profanity, brief nudity, violence and medical gore.
Directed by Michael Apted; written by Tony Gilroy, based on the book by Michael Palmer; director of photography, John Bailey; edited by Rick Shaine; music by Danny Elfman; production designer, Doug Kraner; produced by Elizabeth Hurley; released by Castle Rock Entertainment. Running time: 117 minutes. This film is rated R.
WITH: Hugh Grant (Dr. Guy Luthan), Gene Hackman (Dr. Lawrence Myrick), Sarah Jessica Parker (Jodie Trammel), David Morse (Frank Hare), Bill Nunn (Burke), David Cronenberg (Lawyer) and John Toles-Bey (Bobby).Continue reading the main story


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For the past four years, guys have dominated American Idol. This year, though, the women made a strong case for themselves on the first night of this season's finals round.
If Jessica Sanchez and Hollie Cavanagh weren't favorites before, they are now. With all the girls singing Whitney Houston hits, Hollie, looking for all the world like a modern-day Dusty Springfield, also gave a complete performance of All the Man That I Need.
And Skylar Laine wasn't far behind them, turning Where Do Broken Hearts Go into a finely nuanced Reba McEntire-style ballad. Those three, along with a strong performance from Erika Van Pelt on I Believe in You and Me, could put two early favorites, Elise Testone and Shannon Magrane, into a precarious position going into Thursday's results show, as both struggled to get comfortable with their song choice.
The guys were more evenly matched. Joshua Ledet got I Wish into a gospel groove, while Jermaine Jones continued to prove that the judges made a mistake cutting him, giving Knocks Me Off My Feet a Barry White-meets-Jerry Butler feel. Colton Dixon turned up the heartthrob heat to cover his vocal limitations on Lately, while DeAndre Brackensick showed a different side of his voice with a raspy, reggae version of Master Blaster. Phillip Phillips gave Superstition a rock treatment, while Jeremy Rosado and Heejun Han both struggled to give their ballads the voices they deserved.
The first elimination will run a little differently than in year's past. On Thursday's show, host Ryan Seacrest will announce the guy and the girl who got the least votes, then let the judges pick which one stays and which goes. If the voting goes according to performance quality, their decision could be difficult, since it almost surely will involve singers to which they've grown emotionally attached.
Here's how I rank them:
Nashville-based Brian Mansfield began writing about music for USA TODAY in 1997 and took over Idol Chatter just before the start of Season 8. He co-authored Make Me a Star: Industry Insiders Reveal How to Make It in Music, and while he has never auditioned for American Idol, he did sing Boot Scootin' Boogie at Disney World's American Idol Experience the day the attraction opened (he lost). More about Brian | E-mail Brian


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The Moneylife Team looks at Vandrevala Foundation that deals with mental health
In a crowded city like Mumbai, it is not uncommon to see someone suffering from dyslexia or a mental health disorder. But most people know little about mental health issues unless a close friend or family member suffers from a disorder. Do you know that there are 43 mental health hospitals in India, all set up before 1947? A National Human Rights Commission study says: There are only two kinds of hospitals. The first are 'dumping grounds' for families to abandon their mentally ill member, for either economic reasons or a lack of understanding and awareness of mental illness. The second type of hospitals provides basic living amenities but the treatment focuses on managing patients rather than enhancing their living skills. In addition, there is the stigma associated with mental illnesses and, often, a refusal to acknowledge a problem. Yet, "a massive 8%-10% of the population apparently suffers from major or minor mental illness," says Dr Arun John, executive vice president of the Vandrevala Foundation.
"When we started out with a mission to improve mental health in India over a year ago, we were hoping to write cheques to a couple of non-government organisations (NGOs), be able to review their results four times a year and pat ourselves on the back for making a difference. What ensued was a discovery of the dark corners of disease, the discrimination and the realisation that we had no idea about what we were trying to achieve, let alone how to do it," says Priya Hiranandani Vandrevala, chief executive officer of Hirco PLC, a real-estate investment company.
Founded and fully funded by Priya and Cyrus Vandrevala, the Vandrevala Foundation began operations on 3 August 2009 by launching 'The Mental Health Initiative'. As a first step, it launched a 24x7 mental health helpline. Its mission is to create community-based mental health services, increase awareness of mental health, provide access to improved treatment to every individual irrespective of economic status and enable patients and their families the opportunity for recovery and reintegration into society.
Dr John says, "We are looking for strategic partners —corporates, like-minded individuals or NGOs with the same goals to help us in our endeavour." Interestingly, the Vandrevala Foundation says it is a not-for-profit that works like a 'for-profit' organisation, but for social profits, not economic gains.
"Preventive action is possible and necessary for physical illness, but there is no such thing for mental illness," says Dr John. So the Foundation runs a 24x7 helpline (022-2570 6000) to counsel people in need. It also has senior psychiatrists available for acute psychiatric emergency or complex issues. It helps arrange free ambulance services; helps arrange admissions to mental-care facilities; and follows up to track the status of mentally-ill persons to ensure that they stick to treatment. It arranges meetings with psychiatrists. The idea behind the helpline is the realisation that often people know they need help, but don't know who to turn to.
The Vandrevala Foundation also supports entrepreneurial, social-sector start-ups which local governments are unable, or unwilling, to fund. The Foundation plans to expand its helplines to Pune, Nashik and Nagpur. "The ultimate goal is a pan-India presence," says Dr John. Currently, the Foundation has 12 clinical psychologists as well as five mid-level and four senior psychiatrists on its rolls.
It also has a network of 70 psychiatrists, psychologists and counsellors all over Mumbai who support the cause. It has students who can communicate in English, Hindi, Marathi and Gujarati manning the helplines. It has two tiers of psychiatrists who look into mental illness cases, depending on the severity of affliction. It also plans to set up crisis management face-to-face counselling and support groups and to upgrade mental hospitals in Maharashtra.
If you know of someone who needs help, you can call or email the Vandrevala Foundation.
VandrevalaFoundation:Sigma, 6th floor, Central Avenue,
Hiranandani Gardens, Powai, Mumbai 400 076
Telephone: +91-22-2570 6000
Email: [email protected]
Website: www.vandrevalafoundation.com
Boston: Mukesh Ambani-led Reliance Industries (RIL) has been ranked second in the list of world's 10 biggest 'sustainable value creators'-companies that have been successful in creating the most shareholder value over the last decade-prepared by Boston Consulting Group (BCG), reports PTI.
The list compiled by the global management consulting firm names Brazil-based mining and materials giant Vale as the top value creator worldwide for the 2000-2009 period.
"One side effect of the Great Recession has been to accelerate the ascent of companies from rapidly developing economies to the top ranks of the world's creators of shareholder value," BCG said in the report titled 'Threading the Needle: Value Creation in a Low-Growth Economy'.
The ranking identifies large global companies with a market capitalisation of at least $35 billion that have been the "most successful at sustaining superior value creation over 10 years."
BCG says a decade is the minimum time frame necessary to evaluate the staying power of a company's value creation performance.
The report has also listed companies industry-wise for creating the most value for their shareholders from 2005 to 2009. Starting from a database of more than 4,000 companies worldwide, the report presents detailed analyses of the Total Shareholder Return (TSR) at 712 companies across 14 major industries for the five-year period.
Of the top 142 companies included in this year's global and industry rankings, 81 are located in developing economies.
In a further indication of how emerging economies are pulling ahead of developed countries, the top 10 value creators in the 712-company sample are all from Asia-five companies listed on stock exchanges in China, two in Hong Kong, and one each in India, Indonesia, and South Korea.
Similarly, seven of the top ten large-cap value creators (those with market valuations of more than $35 billion) are listed on stock exchanges in rapidly developing economies of Brazil, Hong Kong, India, Mexico and South Korea.
"Most developing economies are rebounding relatively quickly to their previous growth levels. In contrast, developed economies are entering an extended period of below-average growth with profound implications for how companies create value and which companies come out on top," report co-author Daniel Stelter said.
Indian mining and materials giant Jindal Steel and Power (JSP) occupies the number two spot in the BCG list of the 'Global Top 10' value creator companies for the 2005-2009 period.
RIL again comes second in the 'Large Cap firms' for 2005-2009 of 112 global companies with a market valuation of more than $35 billion.
In industry-specific rankings, auto major Mahindra and Mahindra (M&M) occupies the fourth spot among 38 automotive companies that created most value for shareholders in 2005-2009 period followed by Hero Honda Motors at sixth and Maruti Suzuki India at the seventh spot.
In the chemicals industry, RIL has been named the second biggest value creator of 53 global firms during the period behind South Korea's OCI.
In the consumer goods category, ITC ranks sixth among the top 10 companies, while Larsen and Toubro (L&T) (sixth) and Bharat Heavy Electricals (BHEL) (seventh) are among the 77 companies that have created the most shareholder value in the machinery and construction segment.
Telecom company Bharti Airtel and Infosys Technologies occupy the seventh and ninth spot respectively in the technology and telecommunications category for the 2005-2009 period.
Somany Ceramics earns a healthy RoE of 25% and the stock is reasonably priced
Somany Ceramics...


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I remember when I attended an after school program when I was pretty young. There was an auntie in the cafeteria. She would serve this super simple vegetable stir fry almost every day. Although it doesn't seem very impressive, but when you eat it, it's always so shockingly delicious. What's
CiCiLicious! Blog
How to Make Authentic Sichuan Spicy Chili Oil Recipe
How to make authentic Sichuan spicy chili oil recipe? Today I'm going to reveal Sichuan Chef's secret recipes!
1/4 of a cup of red pepper flakes
A pinch of salt
1/2 of a cup of vegetable oil
1 raw cinnamon
1 tablespoon of star anise
1 tablespoon of Sichuan pepper corns
3 bay leaves
In a bowl, addMORE NEWS!
Featured VideoRead More
Shockingly Delicious Stir Fry Vegetable
How to Make Cantonese Clams in Black Bean Sauce
We are going to make a homey Cantonese dish, stir fry clams in black beans sauce. My Shifu taught me this recipe before, and it looked like this. Then I tried it a couple more times at home, and it looked like this. Can you tell what's different? Ok, let's
How to Cook Chinese Fish & Eggplant Clay Pot Recipe
Sometimes last month, I shared a video blog with you about my journey to become a chef. In the video, my shifu was teaching me on how to make fish & eggplant in clay pot. Many of you asked me to share this recipe. So today we are going to
How to Cook Amazing Seafood Soup?
Today we are going to make this very beautiful orange color seafood soup. Can you guest where the orange color is coming? I'll tell you in just a bit. First let's take a look at the list of ingredients.
1 medium carrot
3 cups of chicken stock
3 slices of ginger
3 pieces of
The Hottest Chilies Steamed Fish Recipe
Duojiao chilies steamed fish is a traditional Hunan dish. The freshness of the fish and the spiciness of the duojiao chillies are united as one body. Let's take a look at this recipe now!
1 whole fish
1 tablespoon of minced ginger
3 tablespoons of chopped chilies paste
1 tablespoon of minced scallions
1 tablespoon
How to Make Zongzi 粽子 & Happy Dragon Boat Festival!
How to make zongzi? And today let's celebrate Dragon Boat Festival with Felicia, Mia, and Jimmy from Off the Great Wall; Chris from China Uncencored; and Ben from Learn Chinese Now! Woohoo~ It's a feast!
36 bamboo leaves
6 cups of sticky rice
36 red dates
Soak sticky rice in water over night. Soak


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The nuclear receptors REV-ERBα (encoded by NR1D1) and REV-ERBβ (NR1D2) have remained orphans owing to the lack of identified physiological ligands. Here we show that heme is a physiological ligand of both receptors. Heme associates with the ligand-binding domains of the REV-ERB receptors with a 1:1 stoichiometry and enhances the thermal stability of the proteins. Results from experiments of heme depletion in mammalian cells indicate that heme binding to REV-ERB causes the recruitment of the co-repressor NCoR, leading to repression of target genes including BMAL1 (official symbol ARNTL), an essential component of the circadian oscillator. Heme extends the known types of ligands used by the human nuclear receptor family beyond the endocrine hormones and dietary lipids described so far. Our results further indicate that heme regulation of REV-ERBs may link the control of metabolism and the mammalian clock.
REV-ERBα was originally identified as an orphan member of the nuclear hormone receptor (NHR) family on the basis of its canonical domain structure and sequence conservation1,2. REV-ERBβ was subsequently identified by its homology to other NHRs and its pattern of expression, which overlaps greatly with that of REV-ERBα. Both receptors have particularly high expression in the liver, adipose tissue, skeletal muscle and brain3–8, where they are transcribed in a circadian manner9–11. The REV-ERBs are unique in the NHR superfamily in that they lack the carboxy-terminal tail (helix 12) of the ligand-binding domain (LBD), which is required for coactivator recognition12 (Supplementary Fig. 1a online). Both receptors act as constitutive repressors of transcription and bind DNA response elements termed 'ROREs'13–15. Although considerably more is known about the function of REV-ERBα than that of REV-ERBβ, the similarities in their expression patterns and DNA-binding and transcriptional activities indicate that they are likely to overlap substantially in function. Nevertheless, more complete understanding of their functional properties has been hindered by the lack of known physiological ligands.
REV-ERBα is a principal regulator of the cyclic expression of BMAL1 (refs. 16–18), a key component of the mammalian circadian clock. Circadian rhythms play an essential part in aspects of physiology and behavior, including the sleep-wake cycle, body temperature, blood pressure and renal function. The circadian rhythms are generated by feedback loops in gene expression in which heterodimers of BMAL1 and CLOCK activate expression of the cryptochrome (Cry) and period (Per) genes (the negative limb)19,20. When CRY and PER reach a critical level of expression, they block the stimulatory effect of the CLOCK or NPAS2–BMAL1 complex on their own genes, thereby completing the loop20,21. The BMAL1 promoter contains two ROREs, and BMAL1 transcription is directly repressed by REV-ERBα16,22. Mice deficient in NR1D1, the gene encoding REV-ERBα, show loss of the diurnal pattern of expression of BMAL1 and alterations in the period and phase of their circadian behavior patterns17.
Clues to a possible physiological ligand for the REV-ERB proteins has come from a study on E75, a member of the NHR family in Drosophila that has been shown to contain a heme prosthetic group23. The oxidation state of iron-heme in E75 determines whether this NHR can interact with its heterodimer partner, DHR3, and this dimer interaction is further regulated by binding of either nitric oxide or carbon monoxide to heme23. The heme in E75 is highly resistant to dissociation, and its forcible removal produces an unstable protein23,24. Thus, heme could be involved in the regulation of E75 activity by influencing its stability, or by functioning as a sensor of redox and/or diatomic gases23. Alignment of the LBDs of E75 and the REV-ERB receptors shows good conservation overall (Supplementary Fig. 1b). Because heme concentrations change in a circadian manner and can entrain the mammalian clock25,26 and because heme is used as a cofactor by proteins that control metabolic functions and the circadian clock including NPAS2 (ref. 27), we have examined whether the human REV-ERB nuclear receptors use heme as their ligand.
We purified recombinant histidine-tagged human REV-ERBα and REV-ERBβ LBD proteins after expression in Escherichia coli. We also prepared Flag-tagged full-length REV-ERBα and REV-ERBβ by transient transfection of human embryonic kidney HEK293 cells. Both cell systems are known to produce heme that associates with heme-binding proteins28,29, and recombinant E75 protein expressed in E. coli has been shown to associate tightly with heme23.
Both full-length receptors and LBDs were extensively purified, and the samples were notably red or pink in color (Supplementary Fig. 2 online). Matrix-assisted laser desorption-ionization (MALDI) mass spectra of these samples showed a heme peak, corresponding to iron-protoporphyrin IX, with the expected mass of 616 ± 2 Da (mean ± s.d.; Fig. 1a and Supplementary Fig. 3 online). The distinct features of heme bound to the proteins were observed by ultraviolet-visible spectroscopy: namely α and β peaks in the 500–600 nm range and γ (Soret) peaks in the 390–450 nm range (Fig. 1b and Supplementary Fig. 4 online). In addition, the associated heme was in the oxidized state because the Soret peak was shifted on addition of the reducing agent dithionite (Fig. 1b).
To characterize the thermodynamics of heme binding to REV-ERB proteins, we used isothermal titration calorimetry (ITC) after removing heme from the purified LBDs. In contrast to Drosophila melanogaster E75, heme can be removed from the REV-ERB LBDs by extensive washing of Ni-NTA–immobilized proteins or through dialysis (Supplementary Fig. 5 online).
Heme-depleted receptors were appropriately folded and fully competent to bind exogenously added heme, as shown by the ITC measurements; both REV-ERB LBDs bound to heme with a dissociation constant (Kd) of 2–3 μM (Fig. 2a,b). The enthalpy of heme binding was approximately −5 kcal mol−1, and the stoichiometry of heme to receptor binding was roughly 1:1. Thus, heme binding to the REV-ERB LBDs is fully reversible. Although the concentration of heme in different tissues is not known, it has been suggested that they vary in a circadian manner29, indicating that REV-ERBs might function as heme sensors.
Nuclear receptor ligands are generally lipophilic and increase protein thermal stability by binding a hydrophobic central cavity in the LBDs. We examined the effect of heme binding on thermal stability of the LBD by using circular dichroism spectroscopy. Heme binding increased the melting temperature (Tm) of each LBD by 4–5 °C (Fig. 2c,d), consistent with ligand effects on other nuclear receptor LBDs, for which it typically increases the Tm by 1–6 °C (refs. 30,31). These results suggested that a change in protein conformation occurs on heme binding to REV-ERB LBDs, and this idea was supported by peptide interaction 'conformation sensing' assays (Supplementary Fig. 6 and Supplementary Methods online).
Taken together, these data are consistent with the hypothesis that REV-ERBs act as physiological sensors of heme concentrations in cells and that heme binding may act as the molecular switch that regulates receptor conformation.
Most heme binding proteins use histidine side chains to coordinate the heme molecule. In E75, mutation of particular histidine or cysteine residue eliminates heme binding23,24, and some of these residues are conserved in REV-ERBα and REV-ERBβ (Supplementary Fig. 1). We individually mutated residues Cys389 (conserved in REV-ERBα and REV-ERBβ), and Cys418 and His602 (conserved in E75 and both REV-ERBs) in the LBD of REV-ERBα. All three mutant LBDs were stable when expressed in E. coli and were purified for ITC analysis of their ability to bind heme.
C389A and C418A showed affinities for heme similar to that of the wild-type LBD (data not shown). By contrast, REV-ERBα LBD H602F showed substantially diminished affinity for binding heme (Kd: wild type, 3.5 ± 0.2 μM; H602F, > 120 μM; Fig. 3). In addition, ultraviolet-visible spectrum analysis indicated that the H602F mutant was not associated with heme (Fig. 3), implying that H602 is essential for heme binding to the REV-ERBα LBD.
To examine the role of heme in REV-ERBα function in cells, we assessed the transcriptional repressor activity of REV-ERBα LBD and the H602F mutant, fused to the DNA-binding domain (DBD) of Gal4 in both HuH7 hepatoma cells and HEK293 cells. Use of the Gal4 DBD chimeric receptors facilitated direct assessment of the transcriptional repressor activity of the LBDs in isolation.
Both fusions were expressed in similar amounts, as determined by immunoblotting (Supplementary Fig. 7a online). There was, however, a complete loss in transcriptional repression activity of the H602F LBD, as compared with the wild-type LBD (Fig. 3), suggesting that heme binding is required for repression.
The lack of ability of the Gal4 DBD REV-ERBα LBD H602F mutant to repress transcription suggested that heme might be required for the repressive effect of REV-ERBα on its target genes. To test this idea, we modulated intracellular heme in human HepG2 hepatoblastoma cells and examined the effects on the REV-ERBα target genes BMAL1 and ELOVL3. We inhibited heme biosynthesis in cells with succinylacetone, a highly specific inhibitor of aminolevulinic acid dehydratase that has been used to decrease intracellular heme in several cell lines and in vivo32–37.
Although intracellular heme is usually tightly regulated, we effectively reduced intracellular concentrations by about 40% using succinylacetone (Fig. 4a). This reduction was accompanied by a 2.6-fold increase in the expression of ALAS1 (Fig. 4b), the gene encoding aminolevulinic acid synthase. Because ALAS1 expression is known to be under negative transcriptional feedback control by heme34, the observed upregulation of ALAS1 on succinylacetone treatment indicates that the decrease in heme is physiologically significant. By contrast, addition of hemin to the cells resulted in a significant (P < 0.05) 33% increase in intracellular heme (Fig. 4a), accompanied by a 40% decrease in ALAS1 expression, again consistent with the physiological response to an excess of intracellular heme. As an alternative to succinylacetone method, the effect of inhibition of ALAS1 expression by short interfering RNA (siRNA) was also examined. We found, however, that there was no correlation between inhibition of ALAS1 and a decrease in intracellular heme (potentially owing to compensatory effects of heme oxygenase) and, as a result, we did not pursue further the siRNA approach for modulation of heme.
In heme-depleted cells, we noted a significant (P < 0.05) increase in expression of BMAL1 (3-fold) and ELOVL3 (5.5-fold; Fig. 4c,d). The increase in expression of REV-ERB target genes that followed heme depletion was consistent with our data on the chimeric H602F LBD, suggesting that an ability to bind heme is required for the repressor activity of REV-ERB LBD. We expected that increasing intracellular heme would result in a decrease in expression of REVERB target genes, owing to an increase in repressive activity. Indeed, raising intracellular heme caused significant (P < 0.05) decreases in BMAL1 and ELOVL3 expression (Fig. 4c,d). These data are consistent with our hypothesis that heme selectively regulates the transcription of REV-ERB target genes.
REV-ERBs repress the transcription of their target genes through recruitment of the co-repressor NCoR38,39. The inability of the H602F REV-ERBα LBD mutant to repress transcription, in addition to the observation that intracellular heme regulates the expression of REV-ERBα target genes, suggests that heme may regulate the ability of REV-ERBα to interact with NCoR. Consistent with this hypothesis, depleting intracellular heme resulted in a decreased interaction of REV-ERBα with NCoR, as determined by co-immunoprecipitation. (Fig. 5a). Addition of 30 μM hemin to the heme-depleted cells resulted in partial recovery of the interaction between REV-ERBα and NCoR (Fig. 5b). Depletion of heme also resulted in a large decrease in NCoR occupancy of the RORE in the BMAL1 promoter, as determined by chromatin immunoprecipitation (ChIP) and quantitative PCR (Fig. 5b). Similar to the co-immunoprecipitation results, addition of hemin to the heme-depleted cells resulted in partial recovery of NCoR occupancy of the BMAL1 promoter (Fig. 5b). It should be noted that the co-immunoprecipitation and ChIP studies were performed in HepG2 cells expressing endogenous amounts of REV-ERBα and NCoR. These data indicate that heme can regulate the interaction of REV-ERBα with NCoR and are also consistent with the hypothesis that heme regulates REV-ERB–mediated recruitment of NCoR to the BMAL1 promoter.
In other types of heme–protein complexes, the heme prosthetic group confers the ability to respond either to alterations in redox state or to diatomic gases. We found that reduction of the heme iron through the addition of 10 μM dithionite did not substantially change the binding activity of the CoRNR box peptide, suggesting that the redox state of heme does not alter LBD conformation (Supplementary Fig. 8a,b online). Addition of pharmacological nitric oxide donors did not affect the transcriptional activity of the REV-ERB LBD (Supplementary Fig. 8a,b). Thus, in contrast to E75, REV-ERBs seem to be insensitive to the redox state and diatomic gasses.
We have shown that heme meets the requirements of a physiological ligand for the human orphan nuclear receptors REV-ERBα and REVERBβ. Heme binding took place in a 1:1 stoichiometry with the REVERB LBDs, as determined by ITC (Fig. 2a,b), and, consistent with the role of heme as a REV-ERB ligand, the receptor LBDs underwent changes in thermal stability and conformation on heme addition (Fig. 2c,d and Supplementary Fig. 6). Modulation of intracellular heme resulted in a change in the expression of REV-ERB target genes (Fig. 4) and in alteration of the interaction of REV-ERBα with NCoR (Fig. 5a). The effects of heme were specific to the REV-ERB LBDs because we observed no effect on other nuclear receptors such as LXR (Supplementary Fig. 6). These results are consistent with the idea that the REV-ERBs function as physiological sensors of intracellular heme. The identification of a member of the nuclear receptor superfamily as a heme receptor indicates that the diversity of endogenous ligands for this superfamily is more extensive than the steroids, retinoids, thyroid hormone and lipids identified so far. The affinity of heme for the REV-ERB LBDs (Kd ≈ 3 μM) suggests that these receptors belong in the category of physiological nuclear receptor 'metabolite' sensors, such as FXR (bile acids), LXR (oxysterols) and PPARs (fatty acids), for which Kd values of 1–10 μM have been observed40.
Although the Drosophila E75 protein also associates with heme, there are many important differences between E75 and the two human REV-ERB proteins characterized here. In biochemical assays, the REV-ERB LBDs show reversible binding with heme and stability in both the ligand-bound and apoprotein state (Supplementary Fig. 6). By contrast, binding of heme to E75 seems to be constitutive and is required for protein stability23. We found that, in the REV-ERB LBDs, a change in the oxidation state of the iron does seem to alter receptor conformation (Supplementary Fig. 9a,b online). In addition, the transcriptional activity of the REV-ERB does not seem to be responsive to diatomic gases. Thus, it seems that REV-ERBs function as sensors of heme, rather than using heme to sense redox conditions or gases.
Variations in intracellular heme, which occur in a circadian manner in mammals25, may allow REV-ERB proteins to modulate repression of their target genes and to shape appropriately the amplitude of the circadian rhythm. REV-ERBα regulates the expression of several genes involved in circadian pathways and in lipid metabolism and adipogenesis16,41–46. Our findings indicate that the REV-ERBs sense and respond to dynamic changes in intracellular free heme and may use heme to integrate the circadian clock and metabolic programs. Importantly, the REV-ERB target gene BMAL1, an essential component of the circadian oscillator, is also essential for adipogenesis47,48. Such links between circadian rhythms and metabolic patterns have been recognized for some time19,48. More direct evidence has come from mice carrying mutations in BMAL1 or Clock. These mice show abnormalities similar to metabolic syndrome including obesity, dyslipidemia and impaired glucose metabolism49,50. Our finding that the REV-ERBs are in fact ligand-regulated receptors suggests that synthetic modulators of the REV-ERB proteins may be developed in the future with the goal of treating human diseases related to coordination of the cellular clock, control of glucose and energy metabolism.
Synthesis of heme, the ligand for the REV-ERBs, is linked to the nutritional status of mammals through the regulation of ALAS1 by the nuclear receptor coactivator PGC-1α (ref. 51). This coactivator is induced by fasting and mediates the transition from glucose to fatty acid use as an energy source and has been shown to integrate the mammalian clock and energy metabolism52. We predict that alterations in PGC-1α–induced expression of ALAS1, along with the rate of heme synthesis, may provide a direct pathway for modulating REV-ERB–mediated regulation of the circadian rhythm and energy metabolism, thereby serving as an additional link between these two crucial and intricately regulated biological processes. Finally, REV-ERBα is involved in the induction of adipogenesis, and we predict that heme has a crucial role in this process. Indeed, heme has been shown to be essential in the induction of adipocyte differentiation53, and our results suggest that this role of heme may be mediated by REV-ERB, which also plays an important part in adipogenesis.
For LBDs, residues 281–614 of REV-ERBα (NM_021724) and 247–579 of REV-ERBβ (NM_005126) were expressed as 6×His-tagged proteins from a pET46 Ek/Lic vector in Rosetta (DE3)-pLysS E. coli cells (Novagen). We added 150 μM 5-amino levulinic acid to the cultures to enhance expression of the proteins. The cells were induced with 0.5 mM isopropyl-β-D-thiogalactoside (IPTG) at 16 °C overnight, collected and lysed in 20 mM Tris (pH 8.0), 500 mM NaCl, 20 mM imidazole and 10% glycerol. The proteins were purified with His-Bind Resin (Novagen) and eluted with 250 mM imidazole. The purified LBDs were dialyzed against 20 mM Tris, 200 mM NaCl and 5% glycerol for subsequent experiments. LBD concentrations were estimated by using the calculated molar extinction coefficient (ε280) of 0.619 = 1 mg/ml (for REV-ERBα) and 0.393 = 1 mg/ml (for REV-ERBβ). The bound heme concentration was estimated by using the extinction coefficient for the heme Soret peak (ε415) of 101.85 = 1 mM.
For mammalian expression of full-length receptors, pCMV7.1_3X plasmids containing Flag-tagged REV-ERBα and REV-ERBβ were transiently transfected into suspension-adapted 293-EBNA cells (CRL-10852, ATCC; 10 l at 106 cells per ml). After 24 h, bovine hemin (H 5533, Sigma-Aldrich) was added at 1 mg/l. After 48 h, cells were collected, frozen at −80 °C, and lysed with Cell Lytic M buffer (Sigma-Aldrich). The proteins were purified by using a Flag M2 affinity (Sigma) column and eluted with 3×Flag peptide (Sigma-Aldrich). The presence of REV-ERBα and REV-ERBβ in the eluted fractions was assessed by immunoblotting using a Flag M2 monoclonal antibody (Sigma-Aldrich). The presence of heme was determined by the Soret band (415 nm).
Soret and α,β absorption spectra were measured on a Cary 50 Bio ultraviolet-visible spectrophotometer at room temperature (22 °C). To reduce the heme group, 10 mM sodium hydrosulfite (dithionite) was added to the protein samples. Spectra of reduced complexes were recorded 5 (for REV–ERBα) or 50 (for REV–ERBβ) min after the addition of 10 mM dithionite.
The REV-ERBα and REV-ERBβ LBDs and the Flag-tagged full-length proteins were first dialyzed against water to remove salt. Protein concentrations of 0.5–1 mg/ml were used for MALDI mass spectrometry analyses. The samples were analyzed at the Biomolecular Facility of the University of Virginia as described in the Supplementary Methods.
We carried out ITC experiments at 20 °C on a MicroCal VP-ITC instrument. For heme binding studies, LBDs were dialyzed extensively against 20 mM Tris (pH 8.0), 200 mM NaCl and 5% glycerol, and then concentrated in the same buffer to 0.050 mM (REV-ERBα) or 0.045 mM (REV-ERBβ). The concentration of hemin solution in the ITC binding studies was 0.466 mM (for REV-ERBα) or 0.561 mM (for REV-ERBβ).
Purified LBDs were dialyzed against 10 mM NaH2PO4 (pH 7.5) and 50 mM NaCl. Measurements were carried out on an AVIV circular dichroism instrument. For temperature melting experiments, a gradient of 20–70 °C was used with 2 °C jumps and a 2-min equilibration time. The change in circular dichroism signal was measured at 223 nm and 224 nm for REV-ERBα and REV-ERBβ, respectively. Protein concentrations were 0.4 mg/ml. Similar measurements were repeated after the addition of 50 μM hemin to the protein samples54.
Heme in HepG2 cell lysates was measured by using a modified QuantiChrom Heme Assay (BioAssay Systems). The amount of heme in each sample is expressed as μM heme per mg of total protein.
Succinylacetone (Sigma) was added to confluent HepG2 cultures at a final concentration of 0.5 mM (ref. 32) for 24 h before cell collection. We added 30 μM hemin (Sigma) to confluent cultures for 24 h before cell collection as described55. Hemin was added either in the absence of succinylacetone (Fig. 4) or in the presence of succinylacetone (simultaneous treatment; Fig. 5).
Quantitative RT-PCR was performed as described56. The BMAL1 (NM_001178) primers were (5′-GTACCAACATGCAACGCAATG-3′) and (5′-TGTGTATGGATTGGTGGCACC-3′). Cyclophilin B (M60857) was used as a standard with the following primers: (5′-GGAGATGGCACAGGAGGAAA -3′) and (5′-CGTAGTGCTTCAGTTTGAAGTTCTCA-3′). ELOVL3 primers were obtained from Superarray.
ChIP assays were performed as described38,57. Chromatin-immunoprecipitated DNA was quantified by quantitative PCR with the following primers specific to the human BMAL1 promoter: BMAL1_prom-for (5′-ATTGGTGGCAGGAAAGTAGC-3′) and BMAL1_prom-rev (5′-GTTGTGTGGCGGCTAGAGAG-3′).
HepG2 cells were treated with succinylacetone (2 mM) or both succinylacetone (2 mM) and hemin (30 μM) for 24 h before immunoprecipitation. Cells were lysed in Triton lysis buffer (20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 1 mM EDTA, 1% Triton X-100, 10% glycerol, 1.5 mM MgCl2, 1 mM dithiothreitol, 1 mM phenylmethylsulphonyl fluoride, 10 mg/ml of aprotinin and 10 mg/ml of leupeptin). Protein concentrations were measured with BCA Protein Assay Reagent (Pierce) and ~0.5 mg of protein lysate was used for each immunoprecipitation. The antibodies used for immuno-precipitation and immunoblotting were anti–REV-ERBα (Cell Signaling Technologies) and anti–NCoR C-20 (Santa Cruz Biotechnology).
HuH7 and HEK293 cells were seeded in 96-well plates at 25,000 cells per well as described58 with the modifications outlined in the Supplementary Methods.
This work was supported by a grant from the National Institutes of Health (GM055217) to F.R. and an unrestricted research grant from Eli Lilly and Company to T.P.B.
Note: Supplementary information is available on the Nature Structural & Molecular Biology website.
AUTHOR CONTRIBUTIONSS.R. produced the wild-type and mutant constructs and, together with P.H., purified the proteins and carried out mass spectrometry and ultraviolet-visible spectroscopy. P.H. and S.K. designed and performed the ITC and circular dichroism studies. K.R.S. performed co-transfection and luminex assays. P.M.R. performed the co-immunoprecipitation and ChIP assays. D.B.M. expressed REVERBs in HEK293 cells. A.K.N and L.L.B. created REV-ERB mutants and performed co-transfection assays. T.P.B. and F.R. conceived and designed the studies and wrote the manuscript.
Reprints and permissions information is available online at http://npg.nature.com/reprintsandpermissions
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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American Tower Corporation (NYSE: AMT) is a large market cap stock with a market cap of 48611.91. It is in the REIT – Diversified industry and sector Financial, with a current P/E of 70.11, a forward P/E of 40.66 and EPS of 1.62. At a stock price of 113.23 (-0.89%) it has a dividend yield of 1.76%.
EPS growth for the last five years have been 8.80%, more recently this last year it has grown by -29.80%. The next year growth is going to be about 36.45% and more long-term 30.05% after five years. EPS growth quarter over quarter is 23.80%. Sales growth for the past five years have been 19.20% and sales growth quarter over quarter is 22.80%.
For performance, American Tower Corporation the past week has seen a gain of -0.33%. For the last month performance for American Tower Corporation is -2.93%. While the last quarter is 7.29% and half year, 24.02%. Finally for the year, performance is 23.08%.
The 52-week high for American Tower Corporation, is at -4.25%, and for the 52-week low it comes to a value of 37.65%. The 20-day simple moving average is -0.98% and 10.96% for the 200-day simple moving average.
Volatility for the week is at 1.40%, and for the month it is 1.13%. American Tower Corporation, has a target price of 129.44.
In terms of debt, long term debt/equity is 2.72, and for total debt/equity American Tower Corporation has 2.76. The gross margin is 70.80%, while operating margin is 32.10%, the profit margin is 13.20%. The current ratio is 0.8 and the quick ratio is 0.8.
Insider ownership is at 0.20%, with instituitional ownership at 95.90%. American Tower Corporation has a payout ratio of 122.10%. With the total shares outstanding coming to 429.32. The shares float is 424.95, with the float short at 1.41%, with short ratio coming to 3.34.
In terms of returns, the return on assets see American Tower Corporation, get 2.50%, with its returns on investment at 5.80%. Return on equity is 10.30%. So will the investors see the target price of 129.44, reached soon?
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Added new stills for the upcoming Korean movie "Penny Pinchers" (2011)
Directed by Kim Jeong-hwan-III
With Han Ye-seul, Song Joong-ki, Lee Sang-yeob, Shin So-yul,...
Previously known as "Although I Was Born" (태어나긴 했지만, Tae-eo-na-gin Haett-ji-man)
In this unemployment crisis, a real stingy girl and a boasting boy start a project to make 200 million won. It is written by rookie director Kim Jeong-hwan-III and chosen movie in 2010 planning development.
Release date in Korea : 2011/11/10
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Added new poster and images for the upcoming Korean documentary "Teacher and the Devils"
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