text:
Acne can occur anywhere on the body, including the back and stomach. Acne results when the sebaceous glands enlarge and produce excessive amounts of oil. This oil plugs the pores on the skin, causing whiteheads, blackheads and pimples to develop. Acne on the stomach may cause considerable discomfort due to irritation caused by clothing and sweating. Most cases of acne can be treated at home with regular washing and other self-care measures. Severe cases require treatment by a dermatologist.
Things You'll Need
- Exfoliating sponge
- Over-the-counter acne medication
Shower daily and wash your stomach with a mild, unscented soap to remove dirt and excess oil. Using an exfoliating sponge on your stomach to help unclog pores and remove dead skills cells, both of which can contribute to stomach acne.
Wear clothing with cotton waistbands. Avoid tight belts or pants if these irritate your acne. Some individuals are allergic to nickel, a metal commonly used in snaps and buttons. If your stomach itches or you develop large acne-like spots in the center of your stomach, you may have a nickel allergy.
Keep your hands off your stomach as much as possible, and do not pick at any acne spots you currently have. Picking at blemishes will increase irritation and inflammation, prolong healing time and may lead to infection.
Exercise daily to improve circulation and flush toxins from your body through sweating. Make certain to shower after exercising to prevent oil from remaining on your skin and clogging your pores.
Drink plenty of fluids and eat a healthy, balanced diet. Fruits and vegetables provide your body with the vitamins and minerals necessary for healthy skin. They contain antioxidants beneficial to those with acne. The American Academy of Dermatology states that there are no specific foods associated with acne development.
Apply an over-the-counter (OTC) acne medication to your stomach each night before bed. Products containing benzoyl peroxide or salicylic acid are typically the most effective at clearing up acne, but these medications may cause redness and drying of the skin.
Try an antibiotic or prescription-strength anti-inflammatory medications if your stomach acne does not improve after 2 months of OTC medication use. If you experience swelling or infection, see your doctor right away. Ask your doctor about having your hormone levels checked if your acne fails to respond to home care or medication. Hormonal imbalances in females, and excessive levels of testosterone in males, may contribute to stomach acne.
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text:
Katy Perry toasts Madonna on stage
Katy Perry surprised fans when she joined Madonna on stage to toast the 'Material Girl' singer at her concert at The Forum in Los Angeles on Tuesday night (27.10.15).
The 'Roar' hitmaker gave a puzzled look as she sniffed and then took a sip of a drink from a banana-shaped container, before passing it to the Queen of Pop to celebrate her latest 'Rebel Heart World Tour' concert.
Referring to her impromptu toast, Madonna later posted on Twitter: "Sharing a Banana with Katy Perry‼ Highlight of my show at the Forum! Thanks Katherine (sic)"

subset: commoncrawl


text:
Fit to Bust: How Great Companies Fail
Tim Phillips, "Fit to Bust: How Great Companies Fail"
ISBN: 074946013X | 2011 | EPUB | 216 pages | 1 MB
Warren Buffet remarked that it takes 20 years to build a reputation and five minutes to ruin it. "If you think about that, you'll do things differently." Journalist Tim Phillips turns his eye toward the most avoidable business disasters of recent history. In each case, the story begins at the point where a disastrous decision was made, and then it examines what happened, why it happened and what could have been different.
Each story highlights a flaw that could affect any organization -- for example, overexpansion, failure to do due diligence, or blindness toward an economic bubble. Phillips then isolates the moment, meeting or decision just before the failure, and asks "What were they thinking?" Engaging and informative, he offers insights on why smart people make bad decisions, as well as on the process of management and decision-making in today's business world.
Using first-hand accounts of the people involved, Fit to Bust discusses business collapses such as Enron, Polaroid, WorldCom, and Woolworth's and explains how changing one decision could have helped avoid disaster.
"Fit to Bust explores the failure of not just any companies, but what are widely regarded as "great" companies. Phillips casts a critical eye on many big names of international stature, offering engaging sketches of each Fit to Bust offers readers an insight into failure on such a large scale that it is almost inconceivable it is repeated time and again. But failure is a way of life that has been all too common in the business world for hundreds of years. Sadly, this is a book that proves it." - ForeWord Book Reviews
"Phillips takes us deeper into the psyche of the business world and exposes some of the unpalatable truths about ourselves as managers, employees or investors." -Chartered Management Institute
"provides an excellent and engaging investigation into the typical causes of business failure, and goes beyond the popular news to examine back-room boardroom decision-making processes and how such decisions can contribute to a company's success or failure." -Midwest Book Review
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text: Bundesliga #BLWorldtour: China tourists Wolves record comfortable win over Changchun Yatai vor 2 Stunden Wolfsburg faced local side Changchun Yatai in their first friendly game on tour in China. Summary - Wolfsburg currently on post-season tour of China. - Defeated Changchun Yatai with two second-half goals. - VfL face PSV Eindhoven in their next friendly match. VfL Wolfsburg ran out comfortable 2-0 winners against local side Changchun Yatai in their opening friendly game of the club's post-season tour of China as part of the #BLWorldTour. Click here to download the official Bundesliga app for free!After a tight first half, Julian Draxler made the difference for the Bundesliga side, delivering two wicked set-pieces for Robin Knoche and Josuha Guilavogui to head the goals in a 2-0 victory for Dieter Hecking's side. Enjoy highlights of the game below. More news Bundesliga Warm welcome for Wolfsburg upon touchdown in China Bundesliga Wolfsburg set for Far East tour Bundesliga Wolfsburg's season heads into extra-time with one-week China trip Free! Get the Bundesliga newsletter delivered straight to your inbox. Sign up today! Subscribe


text:
J.P. Morgan Takes Trading Day Bragging Rights
Shanny Basar, Financial News
Goldman Sachs made twice as many single-day trading losses as Wall Street rival J.P. Morgan last year, according to U.S. Securities and Exchange Commission filings from the two banks.
J.P. Morgan said in its annual 10k filing with the SEC late last month that it made money on 233 out of 260 trading days in 2011. The bank revealed that its average daily trading revenue was $73 million but that on seven days last year it made gains of over $200 million.
At the bank's investor day in New York last week, Jes Staley, chief executive of J.P. Morgan's investment bank, praised the near record performance of the business in 2011: the investment-banking division reported revenues of $26.3 billion and its second-highest profits on record of $6.8 billion in 2011.
In his presentation, Mr. Staley said J.P. Morgan's revenues from its trading business were $19.3 billion last year and added that its trading business was less volatile that those of its peers. Mr. Staley said the volatility of the bank's trading revenues was 25% over the past 12 quarters, compared to 40% for its peer group.
Goldman Sachs, meanwhile, lost money on 54 trading days last year with losses of more than $100 million on four of those, according to its 10k filing. The U.S. bank made gains of more than $100 million on 54 days.


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Language and Linguistics in Pre-Modern China and East Asia
This is an advance summary of a forthcoming article in the Oxford Research Encyclopedia of Linguistics. Please check back later for the full article.
Traditional Chinese linguistics grew out of two distinct interests in language: the philosophical reflection on things and their names, and the practical concern for literacy education and the correct understanding of classical works. The former is most typically found in the teachings of such pre-Qin masters such as Confucius, Xunzi, and Gongsun Long; the latter, in the enormous number of dictionaries, textbooks, and research works, which, as a reflection of the fact that most Chinese morphemes are monosyllabic, are centered around the pronunciations, written forms, and meanings of these monosyllabic morphemes, or zi ("characters") as they are called in Chinese. Apparently, it was the latter, philological, interest that motivated the bulk of the Chinese linguistic tradition, giving rise to such important works as Shuowen Jiezi and Qieyun, and culminating in the scholarship of the Qing dynasty. But at the bottom, the philosophical concern never ceased to exist: the dominating idea that everything should have their rightful names just as they should occupy their rightful places in the universe, for example, was behind the compilation of Shuowen Jiezi and many other works. Further, the development of philology, or xiaoxue ("basic learning"), was strongly influenced by the study of philosophical thoughts, or daxue ("greater learning"), throughout its history.
The above picture, in which Chinese philosophy and philology are combined to form a seemingly autonomous tradition, is complicated, however, by the fact that the Indic linguistic tradition started to influence the Chinese in the 2nd century ce, causing both a remarkable progress in analyzing techniques (especially regarding character pronunciation) and a tension in the underlying philosophy. On the one hand, scholars began to realize that syllables had internal structures, and that the pronunciation of one character could be represented by two others which shared the same initial and rhyme with it respectively. This technique, known as fanqie, laid the basis for the illustrious 7th-century rhyming dictionary Qieyun, the rhyme table Yunjing, and a great many works that followed up. These works, besides providing reference for verse composition (and, consequently, for the imperial examinations held to select government officials), proved such an essential tool in the philological study of classical works, that many Qing scholars, at the very height of traditional Chinese linguistics, regarded character pronunciation as central to xiaoxue, and ultimately to the restoration of ancient ideas. On the other hand, the Chinese never developed a real alphabet for the characters, and despite their continued efforts to interpret the Indic-inspired techniques with Chinese notions, were generally unsuccessful in subsuming these techniques under traditional Chinese philosophy. Thus, the key to understanding the ancients was itself beyond the tradition that the ancients established. This tension facilitated the epistemological changes of Chinese scholarship from the pre-modern to the modern era, when the Chinese came into contact with Western ideas.
Chinese, with its linguistic tradition had a profound impact in ancient East Asia. Not only did traditional studies of Japanese, Korean, and Vietnamese show significant Chinese influence, under which not the least achievement was the invention of the earliest writing systems for these languages, but many scholars from Japan and Korea actually took an active part in the study of Chinese as well, so that the Chinese linguistic tradition would itself be incomplete without the materials and findings these non-Chinese scholars have contributed. However, some of these scholars, most notably Motoori Norinaga and Fujitani Nariakira in Japan, were able to free themselves from the character-centered Chinese routine and develop rather original linguistic theories.


text: The handset got a good review on this very site and has subsequently carved out a nice niche for itself as the consumer-friendly BlackBerry. Now the BlackBerry Pearl 3G is available on the Virgin Media network.According to Pocket Lint, the slimmed-down smartphone is now available on pay monthly deals with Virgin. In terms of specific prices, there are two 24-month deals with a choice of either 600 or 800 minutes, unlimited texts and fair usage for web access for £30 and £35 a month respectively. Alternatively, if you want an 18-month deal, you get the same minutes, texts and internet, but with the prices rising to £35 and £40 respectively.For that you get 3G and Wi-Fi connectivity, a 3.2-megapixel camera, preloaded Facebook and Twitter widgets, access to a wide range of free and paid-for apps through Blackberry App World, plus Media Sync, so you can sync your iTunes or Windows Media Player music library with the handset.If you don't fancy the monthly cost, T-Mobile also offers the device on pay-as-you-go.


text:
The East Bay Rowing Club offers unique and highly effective team building events for companies, nonprofits, military veterans, and other organizations. Our events are designed to enhance individual and team growth through hands-on rowing experiences that have a strong connection to teamwork, trust, and communication. This helps strengthen the cohesiveness of your group while providing each participant key insights into how to work more effectively together.
Rowing is known worldwide as the ultimate team sport. Each rower in the boat must be completely in sync with all the other rowers. If one person is off, the entire boat suffers. We have condensed our extensive experience with rowing into extremely effective—and fun—team building sessions.
Trust Through Rowing
This program combines several aspects of crew into a session that culminates in rowing on the beautiful Oakland Estuary. You'll first learn how a rowing shell works and how critical it is to trust the person sitting in front of and behind you. Then, you'll be introduced to the ergometer, an indoor rowing machine that is used by all competitive and recreational rowers. We'll demonstrate the rowing stroke and your team will spend time practicing the stroke and learning how to move in unison.
Finally, you'll hit the dock, where you'll board an 18-person, flat-bottom rowing barge (very stable) and put into practice what you've learned. The crew rows out into the Estuary to experience what it means to trust each team member in the boat.
No previous experience with rowing is required or necessary.
EBRC provides all equipment, including Personal Flotation Devices, boats, oars, ergometers, and safety instruction.
EBRC's experienced coaching staff are facilitators for our team building events. Each team building session has one or two highly skilled facilitators, depending on group size.
Group Size:
Our team building sessions can have up to 36 participants. The minimum size is 8.
Program Length:
Each session is 3 hours, either in the morning or the afternoon. Start and stop times are flexible, based on the needs of your organization.
~ $100 per person
Food and Beverages:
Meals, snacks, and beverages can be provided at an additional cost.
Contact Us:
To learn more, contact us at email@example.com.


text:
Music Lessons
Mike Visceglia has performed and/or recorded with the likes of: Bruce Springsteen, Jackson Browne, Suzanne Vega, Bette Midler, Christopher Cross, and many others. He'll teach you the secrets of getting a great tone, seamless technique, improve your time and groove, music theory, and reading.
Mike has written bass instruction books for MUSICIANS INSTITUTE as well as being an in-demand session player in New York. He is patient and thorough. All levels are welcome in his midtown-east studio. Call him !


text:
Recent notesRSS feed
Month-first date string no longer parses correctly
The following date format won't be parsed correctly:
Use this instead:
Date.strptime("6/15/2012", '%m/%d/%Y')
Example of usage
str = ActiveSupport::StringInquirer.new('test') str.test? # => true str.foobar? # => false
more_than? instance method
Over the weekend I kept running into instances where I was writing code like this:
Code example
arr = ['hello', 'world'] if arr.length > 2 # do stuff else # do something else end
So I ended up extending the core and adding an instance method of more_than?
Code example
class Array def more_than?(num) length > num end end
arr = ['hello', 'world'] puts "Hello" if arr.more_than? 1
:disable_with is deprecated
Or you can use this way:
<%= submit_tag "Login", data: { disable_with: "Please wait.." } %>
:disable_with is deprecated
Since version 3.2.5 you should not use :disable_with.
Use this:
<%= submit_tag "Login", 'data-disable-with' => "Please wait.." %>
You can remove leading Zeros this way as well
By just adding a - symbol.
%-I %-d
This method does not work.
It's an old problem, reported back in 2010, just reopened issue:
Accepted parameters for validate
Validate method also accepts :on and :if parameters. The default value for :on is :save, the other accepted values are :create and :update
class Comment include ActiveModel::Validations validate :must_be_friends, :on => :create, :if => Proc.new {|comment| some_condition} def must_be_friends errors.add(:base, "Must be friends to leave a comment") unless commenter.friend_of?(commentee) end end
rindex with identically array elements
Code Example
a = [1,1,1] a.rindex( a.min ) #=> 2
similar to clone
See the clone documentation. I see that ActiveRecord is moving from "clone" (3.0.9) to "dup" (?).
Beware nested with_options clobbers!
with_options :foo => :bar do |something| something.with_options :foo => :baz do |inner| what_is(:foo) end end
:foo will be :baz. It will not be [:bar, :baz], for example.
This bit me when trying to do nested with_options for validation where both had :if => something.
If you try to use :id as a non-primary-key field
If you're using this so that you can repurpose :id for another use, it gets hairy: your ActiveRecord::Base subclass will still use :id to refer to your primary key, whatever it be named.
So when you call [my obj].id = 33, 33 is set as the value of your primary key, not your :id attribute!
Starts with capital letter alternative
Just adding an anchor to the regular expression seems simpler (and was faster in my benchmarks, not that that matters much):
'Abracadabra' =~ /^[A-Z]/
STI - Making callbacks trigger in inherited classes
Assuming we have
class ParentClass < ActiveRecord::Base attr_accessible :type end class ChildClass < ParentClass after_save :perform_something end
ParentClass.create({:type => "ChildClass"})
will not trigger ChildClass callbacks. What is more, it will return instance of ParentClass instead of ChildClass.
To resolve this issue, you need to define following module
module ActiveRecord module CallbacksAwareSti extend ActiveSupport::Concern module ClassMethods def new(*args, &block) return super(*args, &block) unless args.first.respond_to?(:with_indifferent_access) type = args.first.with_indifferent_access[:type] if type.blank? or (type = type.constantize) == self super(*args, &block) else super(*args, &block).becomes(type) end end end end end
and include it in parent class
class ParentClass < ActiveRecord::Base include ActiveRecord::CallbacksAwareSti attr_accessible :type end
Inspired by http://stackoverflow.com/questions/4518935/activerecord-problems-using-callbacks-and-sti
Undefined Method `mktmpdir' for Dir:Class
Be sure to
require 'tmpdir'
before using it. Read more at http://mikbe.tk/2011/03/07/temporary-directory.
block only and except
class Journal < ActionController::Base # Require authentication for edit and delete. before_filter :authorize, :only => [:edit, :delete] # Passing options to a filter with a block. around_filter(:except => :index) do |controller, action_block| results = Profiler.run(&action_block) controller.response.sub! "", "#{results}" end private def authorize # Redirect to login unless authenticated. end end
Hidden Field Example
Here's a pseudo code example of a hidden field within an ERB template. A post has many comments and this comment form is in a post's show view. This would set a comment's post_id attribute.
<%= form_for(@comment) do |f| %>
<%= f.hidden_field :post_id, :value => @post.id %>
<% end %>
Starts with a Capital Letter
(or any regular expression you'd like)
'Abracadabra'[0..0] =~ /[A-Z]/ # => true
method to use instead
This may be obvious, but the replacement for this method is csrf_meta_tags.
Example from Code School
module Tweets
class ShowPresenter extend ActiveSupport::Memoizable def initialize(tweet) @tweet = tweet end def username @tweet.user.username end def status @tweet.status end def favorites_count @tweet.favorites.count end memoize :username, :status, :favorites_count end end
MUCH easier way to pluralize without the number...
Just use Ruby!
HTML5 data- attributes using RESTful approach
HTML5 specifies extensible attributes like data-foo="bar" (or as in Twitter Bootstrap data-toggle="modal"), which poses two problems for Rails.
First, if you're using symbol notation in link_to to specify attributes, this fails (dash is not a valid symbol character), so
link_to "Edit", @user, :class => "btn", :data-toggle => "modal"
There are two solutions:
put the symbols in quotes,
use the special :data hash
Solution 1: Quote Symbols
link_to "Edit", @user, :class => "btn", "data-toggle" => "modal"
Solution 2: Use the :data hash
link_to "Edit", @user, :class => "btn", :data => {:toggle => "modal"}
Resulting HTML
Edit
The second is minimally documented, but as a hash, can accept multiple values and is perhaps a little cleaner
Instantiates a new blank object (devoid of methods). The only class method of class BasicObject. see "ri BasicObject"
> o=BasicObject.new (Object doesn't support #inspect)
> o.methods NoMethodError: undefined method `methods' for #<BasicObject:0x0000000267a0a0>
> def o.to_s > self > end
> nil
> o
> #<BasicObject:0x0000000267a0a0>
I got it
Perfect match to work with attr_accessible
To change default locale by the parameter you can set :locale option, like below:
select_date 'user', 'birth', :locale => 'de'
Missed close tag
At the page http://apidock.com/rails/ActionView/Helpers/TagHelper/tag
<tt>.data()</tt> should be instead of <tt>.data()<tt>
Looks like this method has trouble with attributes:
require 'rubygems' require 'bundler' require 'active_support/core_ext' require 'pp' xml = '<test id="appears"> <comment id="doesnt appear"> it worked </comment> <comment> see! </comment> <comment /> </test>' hash = Hash.from_xml(xml) pp hash #=>{"test"=>{"id"=>"appears", "comment"=>["it worked", "see!", nil]}} # Notice how the id attribute on the first comment element doesn't appear.


text:
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Featured Research
Quick Stats
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Can Anything Cool Down M&A in the C-store Industry?
Market forces could slow pace, lower multiples.By John C. Flippen Jr. & John Sartory, Petroleum Capital & Real Estate LLC
The merger and acquisition market in the convenience and gas station (C&G) industry is showing no signs of slowing down and the level of transactional activity in the first quarter of 2015 has started where 2014 ended — red hot.
In the first quarter of 2015, several new acquisitions were announced or closed by some of the most active consolidators in the industry, such as:
- GPM's plan to purchase the 163 sites remaining in Sun Capital's convenience store portfolio;
- CrossAmerica Partners LP acquisition of Erickson Oil Products Inc.'s 64 retail sites;
- Petroleum Marketing Group Inc.'s purchase of Mid-Atlantic Petroleum Properties LLC's retail assets in the metropolitan Washington, D.C. area;
- TravelCenters of America's acquisition of 26 retail sites from Best Oil; and
- Alimentation Couche-Tard's announcement of two new acquisitions in the United States shortly after finalizing its mega-merger with The Pantry.
The public announcements and post-closing industry analysis concerning these acquisitions contained the following themes:
- The buyer was already an active, experienced and major player in the industry and M&A market.
- The seller made the decision to exit the industry because of the "lofty" EBITDA purchase multiples currently being offered by some buyers (i.e., the purchase price was just "too good to turn down").
- The major players in the M&A market are still on the lookout for additional acquisition opportunities.
While all the market forces that have been fueling the merger mania in the industry over the past several years certainly still appear to be in place, we would like to use our crystal ball for the remaining portion of this article to briefly highlight several external events or market forces that could potentially slow down or severely inhibit the current pace of consolidation within the C&G industry and/or lower the purchase multiples.
These are a few of the items that our crystal ball produced when asked the question: What could potentially slow down this hot M&A market within the C&G industry?
Fed Chairwoman Janet Yellen ended the Fed's two-day policy meeting in March by announcing the Federal Reserve was finally removing the "patient" wording from its official policy statement and was willing to consider raising short-term interest rates.
Most market experts now expect the Fed to raise its target federal funds rate for the first time in approximately seven years by no later than the third quarter of this year. The ultimate goal of the Fed will be to push interest rates higher across the market spectrum, from debt obligations maturing in 30 days to 30 years, to avoid the possibility of its current zero interest rate policy fueling economic and market bubbles throughout the economy. The Fed believes the economy is getting closer to where it can grow at a sustainable rate without the added incentive provided by historically low borrowing costs.
This low-interest-rate environment has certainly helped fuel the growth of M&A activity in the C&G industry. As the cost of capital starts to rise in 2015, this should have a suppressing impact on market multiplies that the major consolidators and master limited partnerships (MLPs) have been willing to pay for C&G assets. In addition, as the overall level of interest rates start to rise in the bond market, the ability of MLPs and real estate investment trusts (REITs) to quickly raise the capital needed to continue expanding could be negatively impacted. As rates continue to rise, fixed income investors that typically purchase bonds normally become less interested in higher risk/higher dividend paying classes of investments such as MLPs.
While most capital market participants are currently assuming the Fed will make all the correct policy adjustments over the next several years that are needed to wean the United States off this unprecedented period of accommodative monetary policy, not everyone is sure it can be done without triggering a sharp downturn in GDP and/or causing major convulsions in the capital markets.
Ray Dalio, founder of the $165-billion hedge fund group Bridgewater Associates and one of the most powerful and respected managers on Wall Street, recently warned his clients that the Fed's actions run the risk of causing a 1937-style stock market slump and other dramatic unintended consequences.
Any major decline in share prices and the corresponding P/E (price to earnings) multiples of the most active public players in the M&A marketplace should reduce the purchase multiples this group of consolidators has been recently willing to pay for attractive C&G assets. In addition, if the Fed's actions cause the U.S. economy to significantly slow down or slip into a recession, this will normally cause a company to pull back on its level and pace of acquisition activity.
As we mentioned in our earlier article, most market experts are predicting the C&G industry will continue to consolidate for the foreseeable future and the marketplace will include a sufficient supply of petroleum distributors that are still willing to sell their businesses and exit the industry. However, the terms of a potential sale and the purchase price expectations of many sellers may be becoming too aggressive even for the largest and most active buyers.
Multiples are not the same in every market: Most sellers seem to believe any retail network should trade for approximately the same market multiple no matter where the retail assets are located from a geographic standpoint and/or the overall level of competitiveness of the network. Any rational buyer, no matter its size, has to take into consideration these market factors before making an offer and all acquisitions certainly do not offer the same potential upside opportunities and risks to a buyer.


text: In March EYE, or the Film Museum Amsterdam as they used to call it, is leaving the Vondelpark and moving to its new location on the north shore of the IJ river. This not only means Amsterdam welcomes an impressive and iconic new building, it also means the film museum now has four modern cinemas, a 1200 m2 exhibition space, educational facilities, offices, and the 'Basement' – which includes the digitized EYE collection and a café-restaurant. To celebrate the move to 'Noord' and the opening of the new building, EYE invites film lovers to submit a 20-second EYE-dent conveying the organization's intention to be the gateway to the world of moving images. A selection will be awarded with 10 screenings, exposure on the website, and becoming part of the museum's collection. Deadline is March 15 and you can upload your film here. The strangely amateur image – and museum's visual identity, for that matter – was created by EYE's ad agency Wieden+Kennedy.


text:
Viscoelastic behaviour of human mesenchymal stem cells
In this study, we have investigated the viscoelastic behaviour of individual human adult bone marrow-derived mesenchymal stem cells (hMSCs) and the role of F-actin filaments in maintaining these properties, using micropipette aspiration technique together with a standard linear viscoelastic solid model.
Under a room temperature of 20°C, the instantaneous and equilibrium Young's modulus,
hMSCs behave as viscoelastic solid. Its viscoelstic behaviour are dependent on the structural integrity of the F-actin filaments and temperature.
Mesenchymal stem cells (MSCs), a type of the adult stem cells that can be harvested from bone marrow and other sources such as liver, umbilical cord, placenta, adipose tissue, synovial membrane, amniotic fluid and even teeth
Similar to other cell types, the viability and function of hMSC are influenced by their microenvironment, including the presence of mechanical stimuli. One pathway by which mechanical stimuli may alter gene expression is through a direct physical connection from the extracellular matrix across the membrane and cytoplasm to the interconnected network of cytoskeleton, and eventually reaches the nucleus
As a first step towards a better understanding of the role of mechanotransduction in MSC differentiation, this study aimed to study the viscoelastic behaviour of hMSCs and to obtain baseline data, as they are still scarce to date
Viscoelastic behaviour of hMSCs
Upon the application of a step pressure to a hMSC at the control temperature of 20°C, the aspiration length showed an initial jump, followed by a gradual increase, and eventually reached its equilibrium length after 100 s (Fig.
Figure 1
Micropipette aspiration of hMSCs at room temperature
Micropipette aspiration of hMSCs at room temperature. Images (A-D) are displayed at time t = 1 s, 15 s, 100 s and 200 s after the application of step aspiration pressure, respectively. An initial jump of cell protrusion (A) into the micropipette in response to a step aspiration pressure is followed by an asymptotic creep behavior and eventually reaching equilibrium.
Figure 2
Various patterns of aspiration length at different conditions with respect to time
Various patterns of aspiration length at different conditions with respect to time. hMSCs treated with 20 μM of cytochalasin D at 20°C achieve the longest aspiration length. hMSCs reach the equilibrium aspiration length faster at 20°C than 37°C.
Figure 3
Effects of temperature and cytochalasin D on the instantaneous and equilibrium Young's modulus of hMSCs
Effects of temperature and cytochalasin D on the instantaneous and equilibrium Young's modulus of hMSCs. The stiffness of hMSCs decreases with increasing temperature and increasing concentration of cytochalasin D. *
Figure 4
Dependence of viscosity of hMSCs on temperature and the integrity of actin cytoskeleton
Dependence of viscosity of hMSCs on temperature and the integrity of actin cytoskeleton. *
Effect of Disruption of F-actin
The F-actin network of hMSCs was disrupted to certain degree after treatment with cytochalasin D at 20°C (Fig.
Figure 5
Micropipette aspiration of hMSCs treated with the highest concentration (20 μM) of cytochalasin D (A – D)
Micropipette aspiration of hMSCs treated with the highest concentration (20 μM) of cytochalasin D (A – D). Images are displayed at T = 1 s, 15 s, 100 s and 200 s after the application of step aspiration pressure, respectively. The aspiration length of the cell increased significantly due to the disruption of F-actin filaments.
Temperature effect on the viscoelastic behaviour of hMSCs
hMSCs aspirated at 37°C also exhibited typical viscoelastic behaviour. However, the initial jump of the aspirated length was much shorter than the cells tested at 20°C (Fig.
During the experiment, three other types of viscoelastic behaviour of hMSCs that constitute about 55% of total observations, represented by the aspiration length-time curves, were observed (Fig.
Figure 6
Deviation patterns of non-typical viscoelastic creep response of hMSCs
Deviation patterns of non-typical viscoelastic creep response of hMSCs. After reaching equilibrium length, 34% of the hMSCs experienced a further increase of length with time (deviation 1) while 14% of the hMSCs reached a peak aspiration length before dropping to a lower equilibrium length (deviation 2). A small amount of hMSCs (7%) exhibited a step-wise deformation behavior (deviation 3). Lines connecting data point indicate data trend.
A liquid-like cell with a constant cortical tension will flow easily into a micropipette when the suction pressure exceeds the critical pressure
Table 1
The comparison of mechanical properties obtained for various cells
Cell types
Mechanical properties
Porcine endothelial
114 ± 35
8300 ± 4000
Human chondrocyte
886 ± 289
372 ± 125
2700 ± 1600
present work
Both temperature and disruption of F-actin affected the viscoelastic behviour of hMSCs. The increase in viscosity of these cells is attributed to changes in the mechanical integrity of the cell body spanned by the cytoskeleton. Microtubule, one of the major components of cytoskeleton that provides support for compressive load on cells
As expected, the disruption of F-actin by cytochalasin D has the highest impact on the stiffness of hMSCs, leading to the lowest Young's modulus registered. Actin, the major protein components in eukaryotic cells, plays an important role in cell motility and in maintaining cell shape
Interestingly, three types of deviations from the typical viscoelastic behaviour were observed during the study (Fig.
The second deviation (deviation 2), which is characterized by a peak before reaching the equilibrium length, may arise due to either global or localized cell contraction, by rearrangement of actin cytoskeleton after reaching the maximum aspiration length. The third deviation (deviation 3) with a step-wise pattern could be interpreted as the result of a combination of large cell deformation and contraction during aspiration. These patterns, however, were not observed in the hMSCs treated with cytochalasin D, suggesting that the disruption of actin directly affects the deformation behaviour of cells. The results obtained from these three deviations were excluded in the calculation of Young's modulus and viscosity. It should be mentioned that the mechanisms for the latter two deviations are unclear at present. Further experiments with larger pressure range (pressure range for the present study is 500–1000 Pa) may be desirable in order to show how aspiration length-time curves respond to a range of pressure and subsequently constructing possible mechanisms.
Experiments invloving F-actin demonstrated the importance of cytoskeleton in controlling the viscoelastic characteristics of hMSCs. Systematic disruption of other cytoskeletal components such as microtubules and intermediate filaments using relevant chemicals will be performed in subsequent studies, to provide a better understanding of the roles of each of the cytoskeletal components in relation to cell deformation and mechanical properties. Work is also underway to perform live cell staining using green flouoroscence protein (GFP) tag to establish the relationship between the deformation at cellular and cytoskeleton levels. It should be mentioned that the spatial location of nucleus may contribut to the overall deformation of a cell in an aspiration experiemnt and thus influence the aspiration length-time curve. For instance, if the stiffer nucleus is near the tip of the micropipette, it could be anticipated that the aspiration length would be shorter compared to the case where the nucleus is away from the tip. However, quantiative and systemaic analyses of the effect of nucleus can only be revealed by compuational methods.
In summary, our findings validated the hypothesis that hMSCs behave like a viscoelastic solid material, consistent with other cell types such as chrondocytes and endothelial cells. In addition to typical viscoelastic behviour, three other types of non-typical viscoelastic behaviours of hMSCs were also seen. We have also demonstraed that both instantaneous and equilibrium Young's modulus, as well as the apparent viscosity of hMSCs were sensitive to temperature and the structural integrity of F-actin filaments. The baseline data for hMSCs obtained in this study is expected to facilitate future studies in understanding the relationship between mechanical stimuli and phenotypic alterations of hMSCs, and quantitative description of hMSC behaviour.
Culture of hMSCs and disruption of microfilaments
The hMSC cell line, derived from human bone marrow, was purchased from Cambrex Bio Science Walkersville, Inc., USA. hMSCs were cultured at 5% CO2, 37°C using MSC growth medium (MSCGM) (Cambrex, USA), which contains the mixture of MSC basal medium (MSCBM) and supplements such as MSC Growth Supplement (MCGS), L-glutamine, and penicillin/streptomycin. The cells were cultured for 5 days to about 70–80% confluent before harvesting. Once reached 80% confluence, the hMSCs were harvested with 0.25% Trypsin/EDTA (Invitrogen, USA), applied for 5 minutes. After adding appropriate amount of stem cell medium to neutralize trypsin, the suspended cells are given 1 minute to recover before they are plated on a coverslip (0.17 mm thick) for micropipette aspiration experiments.
hMSCs used in the study did not exceed passage number 9 as hMSCs may lose its multi-lineage potential at a later passage number
Table 2
The corresponding cell number of hMSCs under the different experimental conditions
Concentration of cytochalasin-D, (
Cell number,
20°C (control)
Micropipette aspiration
Micropipette aspiration technique was deployed to study the viscoelastic behaviour of hMSCs by applying a suction pressure on the surface of a cell, which was suspended in hMSC growth medium and deposited on a glass coverslip. A heating stage (Heatable Universal Mounting Frame M-H (Zeiss) connected to a temperature regulator, Tempcontrol 37-2 digital (Zeiss)) was used for the experiment conducted at 37°C. Micropipettes were fabricated and refined by drawing borosilicate glass capillary tubes (1.0 mm outer diameter, 0.5 mm inner diameter, Sutter Instrument Co., USA.) using a horizontal pipette puller (Flaming/Brown micropipette puller, model P – 97, Sutter Instrument Co., USA.) and a microforge (Narishige, Japan), respectively, to obtain a range of inner diameter from 7 to 11 μm, which would maintain a constant cell – diameter to inner – micropipette – diameter ratio. Micropipettes were coated with Sigmacote chemical (Sigma, USA) to prevent cell adhesion on the wall of the micropipettes during aspiration.
Before starting an experiment, air bubbles were removed from the tubing by fluid flow. The micropipette was filled with distilled water through a flexible needle attached to a syringe and placed into the micropipette holder. The suction pressure and the position of the micropipette were controlled by a micromanipulator (Narishige, Japan). The amount of pressure exerted on the cells was measured by an in-line pressure transducer (Model DP15 – 30, Validyne Engineering Corp., USA) with a resolution of 1 Pa and was converted to voltage reading using a demodulator (Model CD280-2C, Validyne Engineering Corp., USA) and a digital multimeter (Model 115, Fluke, USA). Each experimental session was limited to within 1 h.
The initial diameter of cells and the equilibrium pressure, at which point the cells were neither aspirated nor pushed away, were obtained before the start of each aspiration to minimize the error incurred by any drift to the measurement of the pressure. A constant step pressure, Δ
Theoretical modelling
A linear, three-parameter viscoelastic solid model was used to determine the mechanical properties of hMSCs
Figure 7
Schematics of theoretical model of the micropipette aspiration test (Adapted from
Schematics of theoretical model of the micropipette aspiration test (Adapted from
where Φ is the wall function related to the ratio of the micropipette wall thickness to the pipette radius. In this study, which is based on the
Statistical analyses were performed using SigmaPlot version 8.02 software (SYSTAT Software, Inc., USA). Unpaired two-sample (with unequal variance)
Authors' contributions
SCWT and WXP carried out experimental studies, data collection, statistical analysis, and drafted the manuscript, they contributed equally to the present work. GM participated in the experimental setup and data collection while NC prepared cells. KWL and KL participated in the design and coordination of the study, as well as critical reading of the manuscript. All authors read and approved the final manuscript.
This work is partially supported by Singapore-University of Washington Alliance (SUWA) Initiative on Engineering Biomaterials and Tissue Engineering.
From the laboratory bench to the patients bedside: An update on clinical trials with mesenchymal stem cells
Multilineage potential of adult human mesenchymal stem cells
Mechanotransduction at cell-matrix and cell-cell contacts
Mechanotransduction across the Cell-Surface and through the Cytoskeleton
Cartilage induction by controlled mechanical stimulation
Compressive force promotes chondrogenic differentiation and hypertrophy in midpalatal suture cartilage in growing rats
Effects of expansive force on the differentiation of midpalatal suture cartilage in rats
Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues
Mechanical stress promotes the expression of smooth muscle-like properties in marrow stromal cells
Collagen synthesis by mesenchymal stem cells and aortic valve interstitial cells in response to mechanical stretch
Differential effects of equiaxial and uniaxial strain on mesenchymal stem cells
An electromagnetic compressive force by cell exciter stimulates chondrogenic differentiation of bone marrow-derived mesenchymal stem cells
Compressive force promotes Sox9, type II collagen and aggrecan and inhibits IL-1 beta expression resulting in chondrogenesis in mouse embryonic limb bud mesenchymal cells
Matrix elasticity directs stem cell lineage specification
Physical plasticity of the nucleus in stem cell differentiation
Viscoelastic properties of human mesenchymal stem cells: 1-4 September; Shanghai, China.
Viscoelastic properties of the cell nucleus
Micropipette aspiration of living cells
Application of the Micropipette Technique to the Measurement of Cultured Porcine Aortic Endothelial-Cell Viscoelastic Properties
Microtubules can bear enhanced compressive loads in living cells because of lateral reinforcement
Phase-Diagram of Microtubules
Effect of Heat on the Microtubule Disassembly and Its Relationship to Body Temperatures
Nonmuscle Actin-Binding Proteins
The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes
Mesenchymal Stem Cells
Action of Cytochalasin-D on Cytoskeletal Networks
The application of a homogeneous half-space model in the analysis of endothelial-cell micropipette measurements


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Brotte Vieilles Vignes 2009, Châteauneuf Du Pape Aoc
Rhone South
Rhone, France
$49.00 (184234)
92 pts
Critic Reviews (1)
Julian Hitner
Quite likely the best Brotte I have ever tasted, the 2009 Vieilles Vignes is an excellent, slightly fruitier-styled...Sign In to see Critic Reviews. Not a Member? Please Sign Up.


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Pet Owner in Seattle
Hi my name is Sofia and i live in Arbor Heights and i have two dogs 1 his name is Bentley which is an English Bulldog who is 5 years old and the second dog is Nala who is a mixed dog she looks like a Lab but she is a mutt. They are easy and lovable dogs they get along great with others. Just need all day friday and friday night and pick up saturday night. Please text me at 206-200-... if youre interested.
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At 14, pot-smoking, DVD player-stealing Jamie is no angel (though his androgynous good looks get him plenty of attention). He is sent to military school, but soon goes AWOL, spending some rough months in Portland, Ore. (mugging joggers, trying meth), before heading to Memphis by Greyhound bus to visit his gay older brother, Peter, who is dying of cancer. Rapp (Under the Wolf, Under the Dog) tells the story through Jamie's unsent letters, with additional letters from relatives and friends giving more background and context. Jamie, who has ADD, details every step (being taken advantage of sexually, getting jumped, befriending a female-to-male transsexual, losing his virginity) in expletive-filled, stream-of-consciousness narration with insights into seedy roadside America ("I think that as a general rule lonely people give other lonely people money a lot") and his own situation. Whether Jamie will survive his bad luck and make it to Memphis in time gives the story tension, but while Jamie leaves much behind each day on the road, little is found. The teenager's singular voice and observations make for an immersive reading experience. Ages 14-up. (May)Copyright © Reed Business Information, a division of Reed Elsevier Inc. All rights reserved.
Punkzillaby Adam Rapp
An award-winning writer and playwright hits the open road for a searing novel-in-letters about a street kid on a highstakes trek across America.
For a runaway boy who goes by the name "Punkzilla," kicking a meth habit and a life of petty crime in Portland, Oregon, is a prelude to a mission: reconnecting with his older brother, a gay man dying/b>
An award-winning writer and playwright hits the open road for a searing novel-in-letters about a street kid on a highstakes trek across America.
For a runaway boy who goes by the name "Punkzilla," kicking a meth habit and a life of petty crime in Portland, Oregon, is a prelude to a mission: reconnecting with his older brother, a gay man dying of cancer in Memphis. Against a backdrop of seedy motels, dicey bus stations, and hitched rides, the desperate fourteen-year-old meets a colorful, sometimes dangerous cast of characters. And in letters to his sibling, he catalogs them all — from an abusive stranger and a ghostly girl to a kind transsexual and an old woman with an oozing eye. The language is raw and revealing, crackling with visceral details and dark humor, yet with each interstate exit Punkzilla's journey grows more urgent: will he make it to Tennessee in time? This daring novel offers a narrative worthy of Kerouac and a keen insight into the power of chance encounters.
Editorial Reviews
Product Details
- 9780763652975
- Publisher:
- Candlewick Press
- Publication date:
- 12/28/2010
- Pages:
- 256
- Sales rank:
- 579,422
- Product dimensions:
- 4.80(w) x 7.50(h) x 0.80(d)
- Lexile:
- 1300L (what's this?)
- Age Range:
- 14 - 17 Years
Read an Excerpt
March 4, 2008
Dear P,
I'm finally writing you back. I've been carrying your letter around in my pocket so it's pretty wrinkled but you have good penmanship or cursive or whatever they call it so it's still totally readable. It actually looks like Mom's writing and I never knew that about you.
I've been meaning to write back for like weeks I swear P but every time I started to do it I would get distracted like I'd have some shit to do or I couldn't find a pen or something. I've never been much of a writer anyway even though this one time in seventh grade I was in detention for skipping class and I had to do this five hundred word essay on politeness and after she read my essay the woman who was running detention this substitute teacher everyone called Mrs. Boobjob told me I had an unusual gift. She wound up giving my essay to this English teacher Mr. Douglas-Roberts and he invited me into a special composition class but I got kicked out right away for chirping like a bird during this thing called an automatic writing exercise. I haven't really written anything for a while so I hope this letter doesn't suck too bad.
So I'm on a Greyhound bus and the driver's wearing a hockey mask. It's clear instead of white and you can see his skin all slimy and pressed up against the mask. When I got on he said hello and his voice was clogged and small. I think he has some sort of infection on his face and I can't tell if he's black or Mexican.
I'm wearing this hoodie I found the other day and I wish I had something a little warmer. Man I feel like shit. I have the chills and I should've eaten something but I'll have to wait for the next refueling point which the driver said would be somewhere in Idaho.
P I've been living in Portland for five months and I'm not sure how I feel about it. I probably won't really know for years because that's how it works right? You don't really develop feelings about a place till you've left it. It's like a girl or a dog like that black Lab E brought home after his pony league game that dog Sarge. Remember how Mom accidentally backed over him with the Olds and how you said he made that squealing sound? I miss that dog even though he only lived with us for a summer. Remember how you used to do that trick where you would put extracrunchy peanut butter on the sprinkler in the front yard and he would start licking the peanut butter off and then you would turn on the sprinkler and he wouldn't stop even though the water was shooting everywhere and he would flip his weird spotted tongue around all crazy and then you would do the fake Fifty Cent voice and it would be like Sarge was really busting rhymes or something.
To be honest I've never really had a girlfriend to miss. I've gotten off here and there but I'm basically talking about hand jobs. I don't mean to be weird P but in your letter you said how you wanted the truth about stuff even if it's ugly and trust me it's going to get a little ugly. Uglier than my skittery penmanship if skittery is even a word.
I can still feel the effects of the meth that me and this kid Branson did last night. It was my first time trying it and it made everything taste aluminum so I didn't feel like eating anything and now I'm totally fucking starving but I already said that right? To be honest P I'm so nervous I can practically feel my bones rattling around under my skin.
The bus smells pretty bad like mold and breath and piss from the bathroom and disinfectant they used to try to cover it up and the back of the seat in front of me has a sticker on it that says jobops.com which is somehow making the smells worse. Out my window the sky is so dark it's almost brown like a bunch of German shepherds got stuck up there. I imagine them snarling and baring their yellow teeth at this shit world and all of its disappointments. That's pretty much all I can see the sickly sky and rain streaking slantways across the glass and the Rose Garden shrinking in the distance like a lost toy.
There are only about eight people on board and six of them look like they're sleeping with their eyes open. This man three seats in front of me is snoring so loud it sounds like he's drowning in a birdbath and this old black woman keeps crying into an Easter basket. I don't even know when Easter is. Maybe she just likes carrying around Easter baskets. She probably had something in it that she lost like some money or a picture of her dead pet. She's wearing a pink shower cap with little yellow daisies on it and she's sitting about four rows in front of me and her crying almost sounds like Santa Claus laughter. Even though it's March I keep thinking she's going to turn around and scream "Merry Christmas foolish-ass bitches!" like she's been saving up all her sorrow and hatred and this skanky bus is the only place she can let it out.
Man I wish I had that iPod Fat Larkin gave me. I wound up giving it to Branson. He's the guy I did meth with last night. He was my best friend in Portland and the one I will miss the most.
I stole about fifty iPods for Fat Larkin. Me and this kid Bobby Job were Fat Larkin's iPod thieves. Bobby Job has emotional problems and likes to stick mechanical pencils in cats' anuses especially this one cat called Acrocat who sounded like a dental drill when it meowed. The emaciated thing followed Bobby Job around with pure loyalty because he would feed it Popeye's. Bobby Job wound up getting his face bit up by a Doberman pinscher and got sent to the Yakima juvy home up in Washington.
Meet the Author
Adam Rapp is the acclaimed author of UNDER THE WOLF, UNDER THE DOG, a LOS ANGELES TIMES Book Prize Finalist and winner of the American Library Association's Schneider Family Book Award, and 33 SNOWFISH, an American Library Association Best Book for Young adults. He is also an accomplished playwright and was a Pulitzer Prize finalist in 2007. He lives in New York City.
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Written as a series of long, descriptive letters, PUNKZILLA tells the story of a fourteen-year-old on a cross-country journey to visit his dying older brother. Jamie (Punkzilla) is AWOL from military school. His father, a retired Major, convinced his mother that Buckner Military Academy would straighten out their youngest son. Jamie is the first to admit he was out-of-control. His ADD - combined with meth, pot, and drinking - had turned him into a punk. But Buckner is way more than he can handle with its emphasis on morning drills and athletic competitions; it's just not who he is. As Punkzilla boards a Greyhound bus heading from Portland, Oregon, to Memphis, Tennessee, he begins writing letters in a notebook. The letters are addressed to his twenty-seven-year-old brother now dying of cancer. Through the letters, readers learn about family tensions that began when Peter announced he was gay and then left to pursue a career as an actor and playwright. This left middle son, Edward, and youngest son, Jamie, at home with a demanding father and an emotional mother. A few scattered letters from these other family members help fill in the gaps in Jamie's tale. One colorful letter after another reveals cross-country adventures as Punkzilla first travels by bus and then hitches rides with one disturbing character after another. Some encounters are helpful and kind, while others are downright creepy and dangerous. Through the letters is Punkzilla's attempt to make sense of his past and come to terms with who he has become. He has very little idea about his future other than his determination to reach Memphis before the death of his beloved brother. Author Adam Rapp has created a world where readers will live vicariously through Punkzilla's letters. The world he paints is harsh and unpleasant with tiny hints of hope and kindness. Readers will appreciate the difficult life of the road as they follow Punkzilla from Portland to Memphis. At times I was frustrated with Punkzilla's actions and choices, but I was drawn to him and remained committed to find out if he arrived in time to say goodbye to Peter.
Loved it


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Wednesday, March 23, 2011
'Urumi' to open in Hong Kong
One of the most anticipated movies of the year, 'Urumi', will have its release on the 31st of this March. But before its wide release plans, the movie will have its representation and trailer in the Hong Kong festival which starts this Monday. One of the big markets for Asian films, the movie is expected to get selected to all major international festivals, which will make it a globally approved film from Mollywood. The lead actress of the movie, Genelia will represent the movie at the festival.
'Urumi' is simultaneously made in Malayalam, Tamil and English.
SOURCE: http://www.indiaglitz.com//channels/malayalam/article.asp?aid=65127
Posted by Admin at Wednesday, March 23, 2011
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I'm currently in an open relationship with the consistently delicious twoowi.
Books: High fidelity, how to be good-Nick Hornby
1984, animal farm-George Orwell
Yes man-Danny Wallace
The princess bride-William Goldman
The time traveler's wife - Audrey Niffenegger
Watchmen - Alan Moore
Batman:The dark knight returns - Frank Miller
Green arrow:Quiver - Kevin Smith
The last 3 are comic books but they still count. I pity the fool who thinks otherwise, almost as much as I pity the fool who calls them graphic novels.
Movies: High fidelity, fight club, back to the future, forest gump, this is spinal tap, batman begins, spiderman, x-men, 4 out of 5 even numbered star trek films, star wars except episode 1 (god I feel cliché typing that), anything by Kevin Smith except jersey girl, liar liar, the Truman show, toy story, the american president, serenity.
Music: I refuse to classify my musical tastes using a genre system that is ambiguous and inaccurate at best, http://www.last.fm/user/dickiedudeles/ gives a good general idea.
Some of my favourite bands include: Green day, Busted, The ataris, American hi-fi, Oasis, Embrace, Stereophonics, Train, Jimmy eat world, Brand new, Fall out boy, Something corporate, Motion city soundtrack, Coldplay, The beatles, Dashboard confessional, Reel big fish, The postal service, The pipettes, The flaming lips, Eve 6, Jack's mannequin, Thursday, Taking back sunday, Straylight run, Snow patrol, Reel big fish, Less than Jake, The new Amsterdams, The get up kids.
Food: CRISPS! Most meat as well, preferably on a pizza or easily microwaveable, I'm not much of a cook.
Television: Scrubs, smallville, simpsons, futurama, Red dwarf, stargate, Star trek(except voyager), Invader Zim, Pinky and the brain, Johnny Bravo, 2DTV, coupling, the office, time gentleman please, TMWRNJ, fist of fun, firefly, foster's home for imaginary friends, justice league(unlimited), teen titans, doctor who, deal or no deal.
Video games: NINTENDO! In particular zelda, mario(including but not limited to kart) and smash brothers. I like me some PC gaming as well, mainly FPS and strategy games in particular Unreal tournament, half life, command and conquer and civilization. When it comes to arcades anything that involves shooting, dancing or eating dots and dodging ghosts is good with me.
You rock
We have something in common
You have complex and intelligent opinions on ninjas, pirates and who would win in a fight between them as well as many other hypothetical fights, mainly involving superheroes
You know it's teenage mutant NINJA turtles
You can name all the doctor who actors
You are the son of a wealthy murdered Nigerian sultan who needs my help in removing £10 million from the country (but only if you can also name all the doctor who actors)


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Rally Pro Cycling Team
Rally Cycling is the next step forward for a team that has been a fixture in the
peloton since 2007 and has taken more than 260 competitive wins and 550
podiums. The men's team heads into 2016 with the addition of former WorldTour
riders Danny Pate and Evan Huffman. Four more North Americans will join Huffman
and Pate in the new Rally colors: Adam De Vos, an up-and- coming climber hailing
from Victoria, British Columbia; Shane Kline, a field sprinter who rode for the Kelly
Benefit Strategies team in 2009; Rob Britton, a strong general classification rider,
and Emerson Oronte, a Boulder, Colorado-based climbing specialist. These guys are
riding aboard the revitalized Diamondback Podium Equipe bikes.


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Even though it was in the early hours of Wednesday morning when word of Sen. Edward Kennedy's death broke, the outpouring of tributes from friends, family and Senate colleagues was immediate. Already congressional Democratic leaders are vowing to make Kennedy's unfulfilled dream of sweeping new health care legislation his legacy.
"As we mourn his loss, we rededicate ourselves to the causes for which he so dutifully dedicated his life," said Senate Majority Leader Harry Reid, D-Nev. Then, echoing a famous line from Kennedy's celebrated 1980 speech to the Democratic National Convention, Reid added: "The liberal lion's mighty roar may now fall silent, but his dream shall never die."
House Speaker Nancy Pelosi, D-Calif., was more explicit. "Ted Kennedy's dream of quality health care for all Americans will be made real this year because of his leadership and his inspiration," she said.
To Democrats, Kennedy represents the party's liberal ideals. Pelosi hailed his "grand vision for America, and an unparalleled ability to effect change." Reid considers it "the thrill of my lifetime to work with Ted Kennedy," whose legacy of social legislation he hailed in his statement.
"Because of Ted Kennedy, more young children could afford to become healthy," Reid said. "More young adults could afford to become students. More of our oldest citizens and our poorest citizens could get the care they need to live longer, fuller lives. More minorities, women and immigrants could realize the rights our founding documents promised them."
It was not only members of his own party mourning Kennedy's passing.
To California's Republican governor, Arnold Schwarzenegger, he was "Uncle Teddy."
Here's the complete statement from the governor, who is married to Kennedy's niece, Maria Shriver:
Maria and I are immensely saddened by the passing of Uncle Teddy. He was known to the world as the Lion of the Senate, a champion of social justice, and a political icon.
Most importantly, he was the rock of our family: a loving husband, father, brother and uncle. He was a man of great faith and character.
Teddy inspired our country through his dedication to health care reform, his commitment to social justice, and his devotion to a life of public service.
I have personally benefited and grown from his experience and advice, and I know countless others have as well.
Teddy taught us all that public service isn't a hobby or even an occupation, but a way of life and his legacy will live on.
Sen. Bob Bennett, a Utah Republican, summed up the way Kennedy won over colleagues despite political differences. "I grew to appreciate the depth of his commitment to his country and his principles," Bennett said in a statement. "It's clear to all that the Senate has lost an icon whose lifetime of service will be long remembered."
Utah's other Republican senator, Orrin Hatch, was one of Kennedy's most devoted friends in the Senate. Here's the statement he issued early this morning:
Today America lost a great elder statesman, a committed public servant, and leader of the Senate. And today I lost a treasured friend. Ted Kennedy was an iconic, larger than life United States senator whose influence cannot be overstated. Many have come before, and many will come after, but Ted Kennedy's name will always be remembered as someone who lived and breathed the United States Senate and the work completed within its chamber.
Kennedy and Hatch co-sponsored a bill earlier this year to expand national service programs, nearly tripling the number of volunteer slots supported by the government. Hatch insisted that the legislation be named after Kennedy. A prolific songwriter, Hatch also composed two ballads in honor of his friend. "Souls Along the Way," commemorating Kennedy's 1992 marriage to Victoria Reggie, became part of the soundtrack of the movie Ocean's Twelve.
After Kennedy's illness was diagnosed, Hatch wrote a another song for him, called Headed Home.
Update, 7:40 a.m. ET: As day breaks on the East Coast, more tributes are arriving. Some of the most heartfelt are from Republicans.
From former Massachusetts Gov. Mitt Romney, who tried unsuccessfully to oust Kennedy from the Senate seat he occupied for 47 years:
In 1994, I joined the long list of those who ran against Ted and came up short. But he was the kind of man you could like even if he was your adversary. I came to admire Ted enormously for his charm and sense of humor -- qualities all the more impressive in a man who had known so much loss and sorrow. I will always remember his great personal kindness, and the fighting spirit he brought to every cause he served and every challenge he faced. I was proud to know Ted Kennedy as a friend, and today my family and I mourn the passing of this big-hearted, unforgettable man.
Sen. Judd Gregg, a fiscally conservative New Hampshire Republican, recalled the "extraordinary working relationship" he had with Kennedy and gave a poignant description of the void his Massachusetts neighbor's death will leave for his colleagues. "Where his booming voice once echoed through the Senate, there will now be a resounding echo," Gregg said in a statement. "But his memory will never leave us."
(Posted by Kathy Kiely; Photo by Brian Snyder, Reuters)
David's journalism career spans three decades, including coverage of five presidential elections, the Oklahoma City bombing, the 2000 Florida presidential recount and the 9/11 terrorist attacks. He has covered the White House for USA TODAY since 2005. His interests include history, politics, books, movies and college football -- not necessarily in that order. More about David
Follow the Oval on Twitter: @The Oval, @djusatoday.
Also contributing to the Oval: Richard Wolf @richardjwolf; Aamer Madhani @AamerISmad


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For more details on Riders and exclusions please refer to the Individual Rider Brochure before concluding the purchase.
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Insurance is the subject matter of solicitation. This is a savings-cum-protection oriented participating endowment assurance plan. For more details on risk factors, terms & conditions please read the sales brochure carefully before concluding the sale.
Kotak Classic Endowment Plan UIN: 107N082V01, Form No: N082, KTB - 107B003V03, KPTB -107B013V02, ADB - 107B001V02, PDB - 107B002V02, CIB - 107B004V03, LGB - 107B012V02, ADGB - 107B011V02.
Ref. No.: KLI/13-14/E-WEB/237
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Website: http://insurance.kotak.com
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Otaku Vines ("us", "we", or "our") operates www.otakuvines.org (the "Site"). This page informs you of our policies regarding the collection, use and disclosure of Personal Information we receive from users of the Site.
We use your Personal Information only for providing and improving the Site. By using the Site, you agree to the collection and use of information in accordance with this policy. Unless otherwise defined in this Privacy Policy, terms used in this Privacy Policy have the same meanings as in our Terms and Conditions, accessible at www.otakuvines.org/
What personal information do we collect from the people that visit our blog, website or app?
We do not collect information from visitors of our site.
When do we collect information?
We collect information from you when you or enter information, via comments or contact form on our site.
How do we use your information?
We may use the information we collect from you when you sign up for our newsletter, respond to a survey or marketing communication, surf the website, or use certain other site features in the following ways:
How do we protect visitor information?
Our website is scanned on a regular basis for security holes and known vulnerabilities in order to make your visit to our site as safe as possible.
- We use regular Malware Scanning.
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- We only provide articles and information, we never ask for personal or private information like email addresses, or credit card numbers.
Do we use 'cookies'?
Yes. Cookies are small files that a site or its service provider transfers to your computer's hard drive through your Web browser (if you allow) that enables the site's or service provider's systems to recognize your browser and capture and remember certain information. For instance, we use cookies to help us remember and process the items in your shopping cart. They are also used to help us understand your preferences based on previous or current site activity, which enables us to provide you with improved services. We also use cookies to help us compile aggregate data about site traffic and site interaction so that we can offer better site experiences and tools in the future.
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text:
Tropical Weather Discussion
NWS National Hurricane Center Miami FL
2205 UTC Tue Jul 25 2017
Tropical Weather Discussion for the eastern Pacific Ocean from the Equator to 32N, east of 140W. The following information is based on satellite imagery, weather observations, radar, and meteorological analysis.
Based on 1800 UTC surface analysis and satellite imagery through 2045 UTC.
...SPECIAL FEATURES... Hurricane Hilary is centered near 15.5N 108.8W at 25/2100 UTC or about 450 nm S of the southern tip of Baja California moving WNW at 10 kt. Estimated minimum central pressure is 974 mb. Maximum sustained wind speed is 90 kt with gusts to 110 kt. Scattered to numerous strong convection was within 120 nm in the NW quadrant of the system. Scattered moderate isolated strong convection was noted elsewhere within 180 nm in the NW and 90 nm in the SE semicircles. See latest NHC forecast/ advisory under AWIPS/WMO headers MIATCMEP4/WTPZ24 for more details.
Hurricane Irwin is centered near 15.7N 120.1W at 25/2100 UTC or about 720 nm SW of the southern tip of Baja California moving W at 6 kt. Estimated minimum central pressure is 983 mb. Maximum sustained wind speed is 75 kt with gusts to 90 kt. Irwin is a small cyclone with scattered moderate to strong convection noted within 75 nm in the W and 30 nm in the E semicircles. See latest NHC forecast/advisory under AWIPS/WMO headers MIATCMEP5/WTPZ25 for more details.
Tropical Depression Greg is centered near 16.2N 137.9W at 25/2100 UTC or about 1030 nm E of South Point Hawaii moving WNW at 10 kt. Estimated minimum central pressure is 1005 mb. Maximum sustained winds are now 30 kt with gusts to 40 kt. Convective activity associated with Greg has decreased this afternoon with isolated moderate convection noted within 90 nm in the SW semicircle. See latest NHC forecast/advisory under AWIPS/WMO headers MIATCMEP2/WTPZ22 for more details.
...TROPICAL WAVES... A tropical wave is along 98W/99W N of 06N moving W at 10-15 kt. Scattered moderate convection is from 08N to 14N between 92W and 96W.
...INTERTROPICAL CONVERGENCE ZONE/MONSOON TROUGH... The monsoon trough extends from 08N78W to 10N85W to 11N99W to 10N104W. Scattered moderate convection is from 05N to 09N E of 82W.
OFFSHORE WATERS WITHIN 250 NM OF MEXICO... Gentle to moderate NW winds off the Baja California peninsula are expected to continue through Wednesday, as high pressure remains centered N-NW of the area. Seas will remain generally 4 to 6 ft in mixed swell through Wednesday. Thereafter...Hurricane Hilary will begin impacting the zones offshore of Baja California through Friday with increased winds and seas through Friday with large southerly swell to 10 ft. Gentle southerly flow will prevail in the Gulf of California, and moderate southerly flow across the northern Gulf of California.
Nocturnal fresh northerly winds are forecast across the Gulf of Tehuantepec through Thursday night into Friday morning. Hilary is expected to impact the offshore waters beyond 200 nm off the coasts of Michoacan, Colima and Jalisco through tonight, then pass S of the Revillagigedo Islands early Wednesday. Seas of at least 8 ft associated with Hilary are forecast to reach the waters between 107W and 115W in the vicinity of Cabo San Lucas Wednesday into Thursday.
OFFSHORE WATERS WITHIN 250 NM OF CENTRAL AMERICA, COLOMBIA, AND WITHIN 750 NM OF ECUADOR... Across the Gulf of Papagayo, expect fresh winds each night with the assistance of the nocturnal drainage flow through the week, occasionally building max seas to 7 ft in a mix of E wind waves and long period SW swell.
Gentle to moderate S to SW winds are expected across the forecast zones the next several days. Long period cross-equatorial SW swell generating 5-7 ft seas will continue to propagate into the forecast waters through mid week. Another set of cross- equatorial long period SW swell of 6 to 9 ft will reach the waters between Ecuador and the Galapagos Islands Thursday, and the coast of Central America on Thursday night.
REMAINDER OF THE AREA... High pressure is located N of the area with a ridge axis that extends across the forecast waters north of 22N. The pressure gradient between this system and the active zone of tropical cyclones between 13N and 19N will maintain fresh N to NE winds W of 130W through mid-week. By Thursday, cross equatorial SW swell of 8 to 9 ft will spread across the waters south of 10N and east of 110W, persisting into the weekend.
$$ Cobb


text:
We are Looking to Hire 2 Magento Developers with 2-5 Years Experience. ( 1 - 5 yrs)
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text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|"William A. Rowe Jr." <wr...@rowe-clan.net>
|Re: Future tag plans
|Fri, 13 Nov 2009 05:16:25 GMT
Greg Stein wrote: > > The SVN API uses apr_pool_t. That typedef could disappear in APR 2.x, > breaking our 1.x APIs. > > Thus, we cannot and will not support APR 2.x in the SVN 1.x series. could -> cannot/will not ? what a leap. Gheesh, seriously, sounds like your polite point of [oh, and 0.9 but we don't talk about that :-] Maybe apr_pool_t goes poof, most likely it doesn't, and either way it's entirely possible to do this. You are simply being woodenheaded :)
|View raw message


text:
Confessions of a media buyer: what you need to know
By Bob Berting
I have owned an advertising agency for longer than I can remember. As a media buyer, I have had presentations from every conceivable media outlet in the market. As you probably know, ad agency media buyers are extremely busy people. They evaluate thousands of print and electronic media and listen to hundreds of presentations. However, for salespeople who represent publications that are not dominant or significant in their markets, it may be difficult to get an appointment.
Know Who To Contact
To get on the media schedule, it's important to know who to contact at the ad agency. For the advertising salesperson, two groups are important: the media department and the account services department. While 85 percent of your time should be spent with the media department, because it does the actual planning and analysis, the account services department should be kept informed about your publication because it has the day-to-day contact with the client. If your publication is appropriate for several clients at the same agency, you'll have to meet with the media and account personnel on each account.
Out Of Sight, Out Of Mind
This saying is especially true with a corporate or ad agency media buyer. When you are not persistent enough, or allow yourself to be intimidated, you fall into a comfortable rut syndrome, ease off or take a passive approach. The usual thinking is "Well I gave it a good try but they don't understand the importance of our publication" or "They are completely oblivious to our existence—sometimes I wonder about our image."
Indoctrination of the media buyer
Although it is not applicable in my case, advertising salespeople quickly recognize the fact that many media buyers are people in their early to mid-20s who went to college and studied advertising, marketing and public relations, but never understood the importance of a community newspaper. They were indoctrinated to think that mass print media, radio, TV and billboards were the target mediums to consider. Because of that situation, it is obvious that a persistent effort is required to reach the subconscious mind of the media buyer and persuade him or her to strongly consider your publication. I want to emphasize that you must be persistent with the media buyer—show why you should be in his or her marketing plans. Send him or her issues of your paper and flyers about special promotions, take ideas to him or her, be creative, ask the buyer to lunch, make concrete proposals. Above all, be nice, be friendly, be courteous.
What does your image look like?
Think about the image you are projecting. What does your business card look like? How about your media kit? Don't ever think your media kit is not important. As a media buyer, I want to carefully study each publication and all the facets of why I should do business with them. The only way I can do this efficiently is to have a well organized media kit from you. I want to know about your audit information and testimonials from satisfied customers. The advertising salesperson who tries to sell me strictly with emotional appeals and no logic or facts is not going to win points with me. There is a trend among some sales training experts to insist a media kit is not necessary; that all that is needed are carefully selected magic-bullet phrases and the media buyer will be swayed to buy and commit to years of advertising. This is not real-world thinking.
3 things a media buyer wants to know
Let's talk about the realities that a media buyer wants to deal with as far as the advertising salesperson is concerned:
They want to trust and believe the salesperson. Will the salesperson always follow up on every statement that promises action? Are they knowledgeable? Are they creative?
Do they have a well planned and executed advertising program? If your publication is to be the major player in their media mix, you must have a realistic and comprehensive plan for them.
Confidence in the publication is essential. If the media buyer is going to agree to a long range program they can't have lingering doubts about the professionalism of the salesperson, the publication's penetration in the market, readership, and response factors. They must have continual reassurance and updates on these points.
Finally, you can't push the process of creativity. There might be several meetings to go over concepts and to thoroughly understand what the media buyer's needs are. Patience will usually win out and the happy ending is a strong and comprehensive ad program in your publication. © Bob Berting 2011
Bob Berting is a professional speaker, newspaper sales trainer, e-book author, and publishing marketing consultant. His sales record in the newspaper business is impressive. For 15 years, he averaged two cold contracts a week, sold 20 shopping centers on yearly contacts, which tripled the publication's sales volume, and rarely sold a contract that was less than 52 weeks or 1,000 inches during a year. He has conducted more than 1,500 seminars for newspaper staffs, their customers, and state press associations. He conducts tele-seminars with topics that are customized for the newspaper industry. Ask for testimonials from happy tele-seminar customers. Bob's website is www.bobberting.com. He can be reached at 800-536-5408 or email@example.com.
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text:
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text:
Over 80,000 geographical features have already been named in NSW, however, many features still remain unnamed. The Board encourages community members to propose new names for these features.
Generally proposals should have the endorsement of the local council or the government department that administers the area where the feature is located. The name should also comply with the Board's Place Naming policy (PDF 219.2 kb) and Guidelines for determination of place names (PDF 60.1 KB).
Naming proposals should be submitted using the Board's Place name application form (PDF 104.8 KB).
If the proposed geographical name commemorates a person the Commemorative naming form (PDF 100 KB) should also be completed and submitted.
The Board will consider these applications and, if approved, process them per the procedure outlined in the Geographical Names Act 1966.
|Board Meeting Dates
|Date for items to be
included in the agenda
|14 March
|1 March
|23 May
|10 May
|18 July
|5 July
|19 September
|6 September
|21 November
|8 November


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What is Gift Aid? ACT claims the basic rate of tax on donations paid by UK taxpayers. For every £1, ACT gets an additional 25 pence. Genius
How does it work? It's simple. When you tick the Gift Aid box on a donation form, ACT can reclaim tax from the HM Revenue and Customs – at no extra cost to you
How about higher rate tax payers? We've got that covered too! If you're a higher rate tax payer, you can claim the difference between the higher rate of 40% and the 20% basic rate on the total of your donation.
Can ACT claim Gift Aid on all donations? Not quite. We can claim Gift Aid on money for sponsored activities or on donations paid directly to the charity. However, Gift Aid isn't available on donations made on behalf of a group or a collection, on money from ticket sales for an event, or on a donation from a company.
Can ACT claim Gift Aid on my future donations? Absolutely. All you need to do is make a one-off Gift Aid declaration. We'll tell you how it's done.
Find out more about Gift Aid at www.hmrc.gov.uk/individuals/giving/gift-aid.htm
Download the Gift Aid declaration form or make your declaration online
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Momentum Trading on the Indian Stock Market
Gagari Chakrabarti, Chitrakalpa Sen - Momentum Trading on the Indian Stock Market
Published: 2013-03-28 | ISBN: 813221126X | PDF | 110 pages | 4 MB
This study is an exploration of the Indian stock market, focusing on the possible presence of momentum trading. One thing, however, should be noted. While it is true that momentum trading, which tends to generate speculative bubbles, may result in a financial market crash, its nature in contrast might depend on the nature of the economy itself. The study, while exploring the presence and nature of momentum trading on the Indian stock market in recent years, seeks to relate it to significant structural breaks in the Indian or global economy. To be precise, it outlines a potential correlation between the instability in the stock market and the speculative trading on the market, exploring the question of whether it is human psychology that drives financial markets. In the process, the choice of a significant structural break has been obvious: the global financial meltdown of 2007-2008 - a crisis that has often been referred to as the worst ever since the crash of 1929. While analyzing the nature of momentum trading on the Indian stock market with regard to the financial crisis of 2007-08, the study takes into account two major representatives of the market, the BSE (Bombay Stock Index) and NSE (National Stock Index), for the period 2005 to 2012. This study seeks to answer a few important questions. First of all, it tries to unveil the underlying structure of the market. In doing so, it examines the following issues: (i) What was the latent structure of the Indian stock market leading up to the crisis of 2007-08? Does the structure offer insights into designing profitable trading strategies? (ii) Is it possible to construct a profitable portfolio on the Indian stock market? (iii) Is there any profitable trading strategy on the Indian stock market? While exploring these issues, the study delves deeper, breaking the whole period down into two sub-periods, before the crisis of 2008 and after the crisis. The purpose of this division is to determine whether there has been any discernible change in the market structure since the shock.
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text: A citizen science fossil cataloguing web application for built for Manchester Museum. Reading Nature's Library is a citizen science project where anyone can help Manchester Museum catalogue their fossil collection. Images of the fossils are viewed by members of the public who input the label data from the images to build a database of the collection.


text:
types of rocks
jarod shellenbarger pd 4 11/18/14
As described in some of the previous topics, igneous rocks are produced by the crystallization and solidification of molten magma. Magma forms when rock is heated to high temperatures (between 625 and 1200° Celsius) beneath the Earth's surface. The exact temperature needed to melt rock is controlled by several factors. Chemistry of the rock material, pressure, presence of gases (like water vapor) all influence when melting occurs. Most of the heat required to melt rock into magma comes from the Earth's central internal region known as the core. Scientists estimate that the temperature of the Earth's core is about 5000° Celsius. Heat moves from the Earth's core towards the solid outer crust by convection and conduction. Convection moves hot plumes of magma vertically from the lower mantle to the upper mantle. Some of these plumes melt through the Earth's solid lithosphere and can produce intrusive igneous features and extrusive igneous features on the surface. Heat can also be generated in the lower lithosphere through friction. The tectonic movement of subducted crustal plates can generate enough heat (and pressure) to melt rock. This fact explains the presence of volcanoes along the margin of some continental plates.
Metamorphism involves the alteration of existing rocks by either excessive heat and pressure, or through the chemical action of fluids. This alteration can cause chemical changes or structural modification to the minerals making up the rock. Structural modification may involve the simple reorganization of minerals into layers or the aggregation of minerals into specific areas within the rock.
Much of the Earth's continental crust is composed of metamorphic and igneous rocks. Together, these two rock types form the base material at the core of the Earth's major continental masses. Overlying this core are often thick layers of sedimentary rocks. In some regions, this base rock is exposed to the atmosphere and is known as shields. On the Canadian Shield we can find some of the oldest rocks found on the planet (3.96 billion years old). These very old rocks are primarily metamorphic. Metamorphic rocks also are the rock type found at the core of the world's various mountain ranges.
Sedimentary rocks can be categorized into three groups based on sediment type. Most sedimentary rocks are formed by the lithification of weathered rock debris that has been physically transported and deposited. During the transport process, the particles that make up these rocks often become rounded due to abrasion or can become highly sorted. Examples of this type of sedimentary rock include conglomerate and sandstone. Scientists sometimes call this general group of sedimentary rocks clastic. The remaining types of sedimentary rocks are created either from chemical precipitation and crystallization, or by the lithification of once living organic matter. We identify these sedimentary rocks as being non-clastic.


text:
We are simply reaching the point of irreconcileable differences on a host of critical issues in this nation. Dictatorship, dissolution, civil war are the only likely results. I advocate none of these - I am just doing the math.
I just don't see the Constitution fairy coming in and restoring liberty. Not with the domestic enemies in the positions they hold.