text:
Counterfeit money reported
There have been two reports of counterfeit money being used in the Riverland in the past week.
Police are also investigating a number of thefts and vandalism.
A counterfeit $50 note was used at a Loxton business over the long weekend.
Staff at the business found the note when recording their takings for the day and notified police.
Last week a 35-year-old Renmark man was reported for allegedly trying to use counterfeit notes at a Berri bank.
Police are also investigating damage caused to toilets at Apex Park, Lake Vista and the basketball courts at Barmera over the long weekend.
The damage is expected to cost about $1,000 to repair.
Copper piping has also been stolen from a rainwater tank at a Berri church. A stereo player was stolen from inside the church.
Topics: burglary, law-crime-and-justice, crime, vandalism, renmark-5341, barmera-5345, vista-5091


text:
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Continental Agency
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text: Home Gallery Video Audi Stan Lee Cameo School Stan Lee Schools Tara Reid, Kevin Smith on the Art of the Cameo for Audi Online Film Promotes Audi Sponsorship of Upcoming Avengers Film Published on Apr 28, 2015 Editor's Pick Comic book legend Stan Lee also has another specialty -- cameo acting, as we've seen in his notable but minor appearancs in the various Marvel movies -- they include a hot dog vendor, a librarian, a security guard and even a Larry King-lookalike. To help promote its sponsorship of the upcoming Avengers Age of Ultron, Audi makes the most of Mr. Lee's sideline skills in this funny new film out of Muhtayzik Hoffer, which won the brand's social account earlier this year, and directed by Kevin Smith of "Clerks" and "Comic Book Men" fame. In it, he teaches a class of "randoms" -- Smith himself, Tara Reid, Lou Ferrigno, Jason Mewes and Michael Rooker -- the nuances of a great cameo and how such jobs differ from "regular acting." If anyone, he's the one to do it because, as Mr. Smith says, "When it comes to small roles, Stan Lee is the biggest." Mr. Lee has even started to make cameo appearances in Audi's Twitter feed. Muhtayzik Hoffer has been turning out plenty of out-of-the-box ideas for other clients too, including Netflix and Google and was Ad Age's 2014 Small Agency of the Year . For more info on the 2015 Small Agency competition, deadline for which is April 29, head over to Adage.com. Rate this Ad You must be registered to rate the ad. Please Login or Register Now About CreditsDateApr 28, 2015Agency:Muhtayzik Hoffer Client:AudiDirector:Kevin SmithExecutive Creative Director:John MatejczykHead of Production:Michelle Spear NicholsonSenior Producer:Jona GoodmanAssociate Creative Director:Adam LedburyArt Director/Associate Creative Director:Guy LembergProduction Company:CaviarExecutive Producer:Kim DellaraProducer:Adrianne McCurrachEditorial Company:ArcadeEditor:Will HasellColorist:Dave HussyMotion Graphics:Timber Executive Producer:Nicole VisramSenior Producer:Gavin CarrollAudio Company:One UnionSr. Engineer:Joaby DealMusic Company:Travis and MaudeExecutive Producer:Kala Sherman Latest Creativity News on Ad Age Agency Brief: Where Is Everybody? Gallegos United Has a New CCO, Arnold Hires Alfson, Low Joins Ogilvy Beijing 'I Have a Huge Idea:' Wyclef Jean Wants to Work With Brands Related work Audi: Secretariat Venables Bell & Partners Audi: Monster Venables Bell & Partners San Francisco Audi: Enter Sandbox - The Making of


text:
Biting the Wall
A comic novel ostensibly describing the trials and tribulations of a deposed computer center director at a small college. All your standard college types are there -- professors, administrators, spies....
Spies? Well, where there are computers, there are those who are up to No Good. As well as an extravagant assortment of campus savants, all scrambling to aid -- or hinder -- the efforts to sort out the mystery. This is a boisterous romp that will keep you alternately in suspense and stitches.
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text:
Macey Abbott
This is me with my friends back at North East. I went to North East all my life up until this year. This summer I moved to Girard, i went to a Katy Perry concert and I also did a color run. Pretty much the best summer so far, hope the next one is just as fun.
Three things about me
I love Netflix. If I'm not hanging out with friends or doing something with my family then I am in my room watching Netflix. My favorite show that I watch on Netflix would have to be Gossip Girl.
I have liked taking photos pretty much my whole life. I even took a photography class this summer to help me with understanding how to take great pictures. I have been taking a lot of pictures so far and the Erie art museum already emailed me asking for my photographs to look at.
I love traveling. Even though I have only been to New York and Florida I would love to travel the world, see new things and meet new people. If you asked me to travel anywhere I wouldn't care where as long as we would be going somewhere new, I'm down for anything.
My goals
My first goal would be to make a lot of new friends this year, since I am new. I plan on accomplishing this goal by getting involved and putting myself out there. For example Joining a club or even sitting in different places for lunch. My second goal would be to survive high school, so far high school has been pretty easy. I know it will get harder soon. I plan on accomplishing this goal by staying focused in class, being myself, and trying my hardest in and out of the classroom. My third would have to be going to college and getting my dream job. I will accomplish this goal by accomplishing my first two goals.
I would love to...
Travel to London. I have always wanted to go to London to see the different cultures and how people do different things there then in America. Like how they talk, and what they eat. I would love to visit the cool museums that they have there and the amazing restaurants all around London.


text: Forty years ago, the legendary actor Robert Redford starred in one of the most celebrated journalism films of all time: "All The President's Men." Redford and Dustin Hoffman portrayed Washington Post reporters Bob Woodward and Carl Bernstein investigating the Watergate scandal that eventually brought down President Nixon. Now he talks about his new film "truth" about the scandel that caused Dan Rather to lose his job. We also disscus Climate change and the #Oscarssowhite.


text:
784 Kuehnle Avenue, Ann Arbor 48103
See more homes for sale in Ann Arbor, MI
This property has been Sold!
07/29/2016 at $270,000
Adorable west side Ann Arbor ranch with lots of charm & character. 4 bedrooms & 2 full baths. Gleaming hardwood floors throughout except for baths & one bedroom. Updated kitchen with granite tile counters, stainless steel appliances, & pass through to formal dining. Living room & family room with 10' vaulted ceiling. Hall bath with double sinks. Spacious rec room with dry wall & carpet & full bath in basement. Large (500 square feet) studio, workshop or potential guest house attached to 2 car garage. Park-like setting .29 acre lot next to city maintained corner lot (Hollywood Park). Fenced back yard with in-ground pool (which can be removed). One-year home warranty included. Walk to Abbott Elementary School. Convenient to shopping & expressway. Additional Inclusion: Pool table in studio and Exclusion: rods & curtains in 2 big bedrooms and checked valance in smaller bedroom. Concession available at stated price for pool removal or potential repair.
Property Information
- Total Finished SqFt
- 2,019
- Lower Finished SqFt
- 400
- Above Grade SqFt
- 1,619
- Garage Desc.
- Door Opener, Electric, Workshop
- Water
- Public
- Sewer
- Public
- Year Built
- 1941
- Home Style
- Ranch
- Summer Taxes
- $3,324
- Winter Taxes
- $462
- Association Fee
- $0
Rooms and Land
- MasterBedroom
- 1st Floor
- Bedroom2
- 1st Floor
- Bedroom3
- 1st Floor
- Bedroom4
- 1st Floor
- Dining
- 1st Floor
- Family
- 1st Floor
- Kitchen
- 1st Floor
- Living
- 1st Floor
- Basement
- Partially Finished
- Cooling
- Central
- Heating
- Forced Air, Gas
- Acreage
- 0.29
- Appliances
- Dishwasher, Disposal, Dryer, Microwave, Range/Oven, Refrigerator, Washer
- Interior Features
- 1st Floor Master, Cath/Vault Ceiling, Ceiling Fan(s)
- Exterior Materials
- Aluminum
- Exterior Features
- Cable Available, Fenced, Outbuilding
Listing Video for 784 Kuehnle Avenue, Ann Arbor MI 48103
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Listing Courtesy of Reinhart Realtors
Office Address 2355 W. Stadium
Listings last updated: . Some properties that appear for sale on this web site may subsequently have been sold and may no longer be available.
Our Michigan real estate agents can answer all of your questions about 784 Kuehnle Avenue, Ann Arbor MI 48103. Real Estate One, Max Broock Realtors, and J&J Realtors are part of the Real Estate One Family of Companies and dominate the Ann Arbor, Michigan real estate market. To sell or buy a home in Ann Arbor, Michigan, contact our real estate agents as we know the Ann Arbor, Michigan real estate market better than anyone with over 100 years of experience in Ann Arbor, Michigan real estate for sale.
The data relating to real estate for sale on this web site appears in part from the IDX programs of our Multiple Listing Services. Real Estate listings held by brokerage firms other than Real Estate One includes the name and address of the listing broker where available.
IDX information is provided exclusively for consumers personal, non-commercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing.
Provided through Ann Arbor Association Board of Realtors. © 2017 Ann Arbor Associate Board of Realtors Ltd. shareholders.


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Memphis, Tenn.–(BUSINESS WIRE)–July 1, 2005–Elvis Presley Enterprises (EPE)
announced today it has signed the nation's leading ecommerce provider, eFashion
Solutions, LLC, to manage the global web-retailing for officially licensed and
trademarked Elvis products. eFashion Solutions, LLC will be responsible for the
worldwide web-marketing and web-retailing for web, catalogue and fan club sales
for all merchandise that bears the Elvis trademark, likeness and image of Elvis.
"The business of internet retail is already a considerable aspect of our highly
successful website, elvis.com," stated Carol Butler, Director of Worldwide Licensing
for EPE. "After considerable searching, we are convinced that the eFashion Solutions
team can help take us to the next level, particularly internationally."
Over the next 18 months, eFashion Solutions, LLC plans to enhance the business
by leveraging their international platform, including accepting foreign currency
and providing faster and less expensive shipping to Elvis aficionados and fans
from over 120 countries.
eFashion Solutions, LLC (www.efashionsolutions.com)
is a turn key eCommerce service provider which has been successful in creating
profitable flagship web-stores for global consumers for the hottest celebrities
of the day including Jennifer Lopez, Eminem, Russell Simmons, Kimora Lee Simmons,
JayZ and Damon Dash, Rapper Nelly and others. Launching 5 1/2 years ago with $3,000
and an idea, the husband and wife team of Edward Foy, Jr. and Jennifer Silano-Foy
now employ over 100 in their Secaucus, NJ-based 71,000 sq. ft. warehouse/call
center/design facility with revenues reaching $30M in 2004 with additional growth
expected in 2005.
"eFashion Solutions, LLC will assist EPE in continuing and expanding the legacy
of Elvis Presley," stated Edward Foy, Jr. CEO. "Our team believes foremost in
high profit margin through leveraging brand integrity. And, although he passed
away 28 years ago, Elvis continues to be the most recognized image in American
pop culture and beloved around the world. The demographics of his fans are growing
younger, so it makes sense for EPE to expand their business with a young organization
with hip clients such as ours."
Elvis Presley Enterprises, Inc. is based in Memphis, with additional offices in
Los Angeles. In addition to Graceland and its related attractions in Memphis,
including the Heartbreak Hotel, EPE is aggressively involved in a worldwide licensing
program, merchandising, music publishing, and television, film, video and internet
projects. For more information on EPE and Graceland, visit www.Elvis.com.
EPE is a wholly owned subsidiary of CKX, Inc., a publicly traded company listed
on the NASDAQ National Market System (NMS) under the ticker symbol CKXE.


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Luxe lipsticks
When it comes to make-up, every woman sets her own budget. For some it's the bare minimum, for others a necessary luxury they're willing to splash their cash on. Lipstick is a product where many would argue you get what you pay for. With designer brands charging huge amounts for theirs, we take a look at the market's most luxurious lipsticks.
Dolce & Gabbana
D&G is a favourite for both beauty and fashion among celebs, recently teaming up with Sophia Loren on a lipstick. The brand currently offers five lipsticks, a liner and two glosses. It promises superior performance thanks to a formula that moisturises lips and a rounded shape that makes for easy application. D&G lipsticks retail for around 28 Euros.
Even just hearing the word Chanel conjures up visions of luxury and many women love bringing a touch of designer class to their make-up bags with a Chanel lipstick. The brand currently boasts four versions: Rouge Coco, Rouge Coco Shine, Rouge Allure and Rouge Allure Velvet. The website offers application tutorials and product suggestions for matching your lipstick to the rest of your make-up - clever! A Chanel lipstick will set you back roughly 35 Euros.
Estèe Lauder
Luxury beauty brand Estèe Lauder is a favourite of supermodel Kendall Jenner's (she's even leant her name to one of their lippies), so it's no wonder more and more women are splashing out on the company's luxe lipsticks. Multifaceted pigments promise to help sculpt the lips, with high-intensity colour, hydration and six hours of wear also on the list of pros. Estèe Lauder lipsticks retail for around 35 Euros.
With a selection of six lipsticks, a lipliner and four lip pencils, there's plenty for lovers of luxury lip products to choose from at NARS. The lightweight formula of its sheer lipstick helps it glide on easily, while conditioners nourish and protect your pout. You can get yours for around 28 Euros.
Iconic British brand Burberry isn't just known for its trench coats - it's also big on its luxury lipsticks. The Burberry Kisses product comes in 28 vibrant shades and promises six hours of "continuous hydration" for a price of about 35 Euros. There's also the Lip Mist for a sheerer finish at around 32 Euros and the Lip Velvet, a matte product, at around 36.50 Euros.
Cover Media


text: 11/23/16 2/09/13 POPSUGAR Fashion Joseph Altuzarra Joseph Altuzarra Runway | Fashion Week Fall 2013 Photos Altuzarra Fall 2013 Runway February 9, 2013 by Marisa Tom 19 Shares Chat with us on Facebook Messenger. Learn what's trending across POPSUGAR. View on One Page Photo 4 of 39 Start Slideshow ADVERTISEMENT () Share This Link Copy What's Your Reaction? Thanks for your reaction Don't forget to share this with your friends! ← Use Arrows Keys → 4 Altuzarra Fall 2013 Previous Next Joseph AltuzarraNew York Fashion WeekFashion Week


text:
POSTED BY: RICHARD SHERMAN in BLOG
With Thanksgiving coming up, I just wanted to take some time and reflect on this year and what I'm grateful for in life. We all need to appreciate these times with our loved ones.
This year has been a rollercoaster. Right after the Super Bowl, we welcomed our son Rayden into this world. Having a child just changes your perspectives on everything. I'd like to think I've come into myself as a father. Football has always been a part of my life, but it's an extraordinary thing to be father. It is more important than anything. I'm so thankful for him, Ashley, and my family who make everyday so special.
I wouldn't be where I am today without you loyal 12s, this has been a rocky season but you have stayed by our side. I'm thankful for the learning process and the obstacles we have faced because they have only made us stronger as players and people.
This is the time of year to cherish with family and I definitely am doing that. Family and health are our greatest gifts and I'm always grateful I can wake up everyday and play a sport I love because I know it won't last forever. I'm going to go out there and finish the season strong. I hope you all have a Happy Thanksgiving and happy holiday season with your loved ones.
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Data from: Phylogeny of salmonids (salmoniformes: Salmonidae) and its molecular dating: analysis of mtDNA data
Date Published: June 17, 2013
DOI: http://dx.doi.org/10.5061/dryad.r42qf
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text:
Wednesday, September 13, 2006
Many gifted high school kids already take some college classes as a way to accelerate their education (indeed, AP classes are supposed to be at the college level, and some 60% of high schools now offer them). Now a school district in California is proposing combining a high school with a junior college to allow kids to earn a high school diploma and a 2-year degree at the same time. While many gifted kids have their eyes on 4-year-degrees, I think this close relationship between a high school and a college would be a great model for other districts.
You can read about Chico, California's efforts here. You can also read some complaints about the project here.
This particular letter-writer doesn't like the idea because kids will be giving up "friends and school activities." But one of the big complaints about the last few years of high school for many bright kids is that they're a holding pattern. You're pretty much grown up, but not entirely, from a legal perspective. You're probably ready for college work, but that requires a real push from parents or the kid to make that happen (will you take a bus to a college? Walk? will the college say yes? who pays?) Combining a high school with a junior college not only allows bright kids to move ahead, it allows kids at the junior college to brush up on subjects they might be spotty on. It's an unfortunate truth that approximately 40% of community college students must enroll in some sort of remedial course. If such classes are already being offered at an attached high school, both the students and taxpayers could save some money.
Of course, many concepts sound better in theory than in practice, but I think this one's worth a try.
3 comments:
Laura, you are so right about these assumptions people are making. Why can't we focus on the needs of each child rather than assume what works for one child works for all?
- Tony Plank said...
You are quite correct that this is worth a try. But truly almost any kid can benefit from a more customized educational opportunity. You would hope that at least the top kids can have the benefit of some minimal customization such as this.
My wife was actually stripped of Salutatorian *because* she took college classes while in High School. The honor was given to someone who never took an accelerated class of any kind but who had more "grade point hours". Oh, and she received straight A's in the college work as well. I often think about this and wonder how much things have changed since. I certainly hope it has changed a great deal.
Evidence of change is the gifted program which my Son is in. He is very lucky. But as happy as I am for him, it still makes me sad for all of the kids at every level of the academic achievement strata who would benefit for something more closely tailored to their individual needs. Someday maybe we will collectively wake up and realize that kids really are our future and start making appropriate investments in them.
I recently took my 14 year old son on a service trip to a small village in Guatemala. There many children can't attend school until they are 10 or 12 years old becuase they have to work in the fields or help care for other family members. I spent time touring the schools there and talking to teachers. I discovered that when children enter school - at whatever age they are then - they are placed in the grade that is appropriate. Thus you will find 12 year olds in the second grade and so on. Image how much better our educational system would be if we did the same thing.
While many districts give high school students post secondary options and pay for those college classes, there are many middle school students (like my son) who are ready for college course work too. It is sometimes difficult to get districts to pay for those. It is imperative that GT parents lobby the districts to create partnerships with the colleges in their areas to facilitate the advanced education for these students.
Also, remember that the power lies with parents. We often forget that and take "no" for an answer. Public schools will listen if enough pressure is placed on them by enough parents. In our area many of us chose to boycott our state test until we got answers to our need for better GT education. We had a dialogue started the next day!
While I don't like to "get in their faces," it is someetimes necessary because gifted education is the stepchild of our educational system.


text:
Warren Yadon Photographer
djazzmilla9Model Female Lisbon, Lisboa e Vale do Tejo, Portugal
My Website: WebsiteMy MM URL: http://www.modelmayhem.com/djazzmilla9
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With 1.7 million freelancers, we'll match you with the perfect talent.
High quality XHTML/CSS cross-browser W3C standard
Your beautiful design/PSD sliced and hand-coded into a W3C-compliant, perfect-in-every-browser website Get your website coded into HTML+CSS+jQuery
Every web page I produce is hand-coded, W3C compliant, and cross-browser compatible.
Improve your search engine rankings with Semantic HTML. Semantic HTML is a crucial part of Search Engine Optimization (SEO) and is a must for any website. We only code using Semantic HTML with proper use of H1..H6, P, OL, UL, LI, DL, EM, STRONG, and other HTML...
Psd to html


text: This is a fascinating guide to the quality of different full-canvas suits, published in 1949 by the U.S. government. It also has a guide to fabrics on pages 6-7. Fused suits make no appearance in this volume. You can read the book here: http://books.google.com/books?id=rx...ce=gbs_ge_summary_r&cad=0#v=onepage&q&f=false Some interesting observations: hand-made buttonholes are often identified today by their rough appearance on the non-facing side. The guide points out that the best hand-made buttonholes should be finished smoothly on both sides (doubling the work and expense for the tailor). Not all canvases are made the same: The quality of a coat front: For the rest of the book: http://books.google.com/books?id=rx...ce=gbs_ge_summary_r&cad=0#v=onepage&q&f=false


text:
posted by sand .
The temperature in a room is kept at a constant 20 °C but the humidity is only 20%. If the room has a volume of 30m³, and the room's door and windows closed, how many kilograms of water must be put into the vaporizer to increase the humidity from 20% to 85% ( the molecular weight of water is 18)


text:
Home » About
Bieicons.com is the most suitable website for you who are looking for the best inspiration of the home and interior design and it will inspire you. This website is also made in order to fulfill your need of the amazing home decorating ideas. To have a modern house design is not an easy thing and you need inspiration.
This website is very suitable for you. There are many room decorations and they are posted well in this website. Here we also share inspiration about the modern living room, bedroom, bathroom, dining room, as well as kitchen. They are shared well here and they also posted in the best and organized post. In this website, you can find hundred posts about the home living and its decoration.
Besides that, there are several categories in this website and they contain fresh and inspiring ideas. Those posts and articles are written by Kayla Laurie Varner. She has been an author that has posted more than hundred articles. She becomes a great editor in this website as well. Her article, the post that we are provided are the fresh and inspiring article. There are also many articles of hers that have been rated 5 and it is awesome.
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text:
CHICAGO - Daniel Kilgore will be in uniform Sunday against the Bears and could make his season debut after getting activated to the 53-man roster Saturday following extensive rehab for his fractured lower leg suffered last season that required two surgeries.
The 49ers said Kilgore isn't likely to start, indicating he might rotate in, the similar way Andrew Tiller did at right guard with Jordan Devey, who shared snaps for four weeks before Tiller took over the full-time job two weeks ago following the bye week in Seattle.
Otherwise, there are no surprises among the inactives. Receiver Jerome Simpson is inactive for the second time in the last three weeks after serving his six-game suspension to start the season. Additionally, undrafted rookie receiver DeAndrew White will not play, bringing into question who would replace Bruce Ellington as return man should he suffer an injury. It's likely Quinton Patton would be up for that role.
Carlos Hyde and Garrett Celek were ruled out Friday and did not make the trip. The team has kept Hyde on the roster despite the stress fracture in his left foot causing him to miss his fifth-straight game. Instead of placing Hyde on injured reserve to activate Kilgore, the 49ers waived inside linebacker Shayne Skov.
With Skov gone, newcomer Ray Ray Armstrong will be in uniform for the first time as a 49ers against the Bears. For Chicago, former Pro-Bowl safety Antrel Rolle will not play while dealing with a high-ankle sprain.


text:
Mini Goals Chalkboards
Get Some Stuff Done!
Get Some Stuff Done!
Mini Goals Chalkboards is a product I designed to help even the biggest procrastinators get some stuff done. Leaving just two spaces for your daily goals helps keep the nasty long to-do list seem more manageable while allowing you to leave some room in the day for the fun stuff you would like to do. Take a break from your busy schedule for a quick walk, lunch outside or write a haiku. Many designs and colors to choose from, for sale on my Etsy shop.
Thank You!


text:
How To Find That Perfect Swimsuit For Your Cruise
The African American population within the United States has begun to condone and embrace plastic surgery in much better numbers through the previous a number of years. These are attractive, gifted, and successful lady exhibiting the world that you do not need to be a dimension zero to succeed in life. Cheerleaders, Alice In Wonderland, French Maids, and several different costume sorts are available in plus sizes, with equipment to make the costumes actually plus size ariel costume pop. Finally, the final food you should embody extra often in your six pack abs weight loss plan is broth based soup. The basic make up of a Navy costume is to incorporate blue or black, white, and gold. With Donnavinci plus dimension clothing you may look your finest not solely on particular occasions, however each time with the plus dimension fashions they provide.
If you want to flaunt these assets that a regular sized costume would, it is best to be able to. The costumes for males apply just the identical. From plus measurement to slim, from youngsters to adults, the need for shop fittings that provide variety and inclusiveness will solely have a tendency to learn the retailer. Plus size robes and robes are perfect to put on over sexier and skimpier clear thongs or panties. Morrison bared his abs in a Gap shirt with Polo Ralph Lauren chinos as he teamed up with scorching plus size absinthe fairy costume Brazilian mannequin Raquel Zimmerman for Vogue journal. In this comparative examine of men, they discovered that there was no hyperlink between the scale of a man's toes and the scale of his penis. This is something that some tend to overlook and end up shopping for a measurement that is either very tight or much looser than the size of the foot which causes extreme back pain and ankle damage.
Any transfer you do goes to work your abs together with so many different muscle tissues in your body, simply keep in mind to keep your decrease stomach pulled in always. Be it with Halloween, Thanksgiving, Bachelors' nigh out, or a mates reunion – for each event, there are fitting adult costumes accessible. Padded: If her boobs are smaller, then a padded bra will add help and cup size for a fuller look. Exclusive range of made to order plus measurement Indian garments plus size satin dress is accessible on-line just for you. You additionally have to have a really feel for what type of plus dimension formal wear appears good on you. As soon as you are taking the French Maid attractive adult costume out of its packaging, you may feel as though you're a completely totally different individual. The article details out some helpful data on the salwar kameez online market.
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text: Hi! Im selling my 2007 exmark 60" 31hp (vanguard vertical shaft). It has 1377 hours. The deck has some rust spots, but other than that looks good. The right arm strut (i call it the deck stabilizer) is hanging down because the balljoint threads are worn out. I believe a new balljoint is around 30 dollars. Other than that the mower is ready to go. Please pm me your email address or phone number and i will gladly send pictures (im so sorry, but i don't know how to post on here). Asking 6200 or best offer thanks


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Download the Game Design and Indie Game Marketing Freebook 07/19/17GameDev.net and CRC Press have teamed up to bring a free ebook of content curated from top titles published by CRC Press. The freebook, Practices of Game Design & Indie Game Marketing, includes chapters from The Art of Game Design: A Book of Lenses, A Practical Guide to Indie Game Marketing, and An Architectural Approach to Level Design. The GameDev.net FreeBook is relevant to game designers, developers, and those interested in learning more about the challenges in game development. We know game development can be a tough discipline and business, so we picked several chapters from CRC Press titles that we thought would be of interest to you, the GameDev.net audience, in your journey to design, develop, and market your next game. The free ebook is available through CRC Press by clicking here. The Curated Books The Art of Game Design: A Book of Lenses, Second Edition, by Jesse Schell Presents 100+ sets of questions, or different lenses, for viewing a game's design, encompassing diverse fields such as psychology, architecture, music, film, software engineering, theme park design, mathematics, anthropology, and more. Written by one of the world's top game designers, this book describes the deepest and most fundamental principles of game design, demonstrating how tactics used in board, card, and athletic games also work in video games. It provides practical instruction on creating world-class games that will be played again and again. View it here. A Practical Guide to Indie Game Marketing, by Joel Dreskin Marketing is an essential but too frequently overlooked or minimized component of the release plan for indie games. A Practical Guide to Indie Game Marketing provides you with the tools needed to build visibility and sell your indie games. With special focus on those developers with small budgets and limited staff and resources, this book is packed with tangible recommendations and techniques that you can put to use immediately. As a seasoned professional of the indie game arena, author Joel Dreskin gives you insight into practical, real-world experiences of marketing numerous successful games and also provides stories of the failures. View it here. An Architectural Approach to Level Design This is one of the first books to integrate architectural and spatial design theory with the field of level design. The book presents architectural techniques and theories for level designers to use in their own work. It connects architecture and level design in different ways that address the practical elements of how designers construct space and the experiential elements of how and why humans interact with this space. Throughout the text, readers learn skills for spatial layout, evoking emotion through gamespaces, and creating better levels through architectural theory. View it here. Learn more and download the ebook by clicking here. Did you know? GameDev.net and CRC Press also recently teamed up to bring GDNet+ Members up to a 20% discount on all CRC Press books. Learn more about this and other benefits here.
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About OrenGL
OrenGL replied to demonized's topic in Games Career DevelopmentGal, I came to the US to pursue a career in the games industry, as you are interested in doing, but I did my computer science degree in Israel before coming. Other than getting good grades the most important asset in helping me get my first job was my game demo. At a game specific school you'll often write a few game related projects you can show off when seeking a job. However I still would recommend getting a traditional computer science degree, since I do believe that it prepares you better for solving new problems, which is a much more useful skill in the long run. On caveat is that it is not uncommon to see a graduate who does not know how to program. When someone hires you they want you to be effective. Make sure you you complete your degree feeling comfortable programming in C++ and can prove it. As far as schools go, try and find a school in a city that has a lot of developers. San Francisco, Seattle, Austin, LA. The more the better. These cities host large game related conferences (such as GDC, E3 or PAX) and many small ones too. It will also make it easier for you to find an internship. Good luck!
OrenGL replied to OrenGL's topic in Math and PhysicsThanks Maze. I'm processing the math, which may take a while. I'll be sure to get back to you if I have questions :)
OrenGL replied to OrenGL's topic in Math and PhysicsThere certainly is a huge variety of material on the subject, which is why I'm seeking help to point me to the right direction. I'll go over the wikipedia article thanks! Calculus and Linear algebra don't scare me, although it been a while since I've done any deep academic work, both have always been fun. Programming in C/C++ is second nature, I can get by in Python and C# and probably anything else if there was reason to. First I'd like to understand of the theory behind FEM and know what areas I would need to learn more deeply so that I could write a 2D solver. If I were to compare this to rigid body physics, I'd want to know that I needed to learn a bit about collision detection and response, integration methods, contact resolution, ext. At the very least I could write some pseudo code describing how an FEM solver works and then dig into the details of each part. You'd probably be better qualified to label the problems I'd like to solve. I can describe them with the features of the sample app I had in mind. For example: 1. A metal rod that could bend by user interaction and return to its original shape (completely or partially). 2. A ball that could inflate or deflate. 3. Being able to launch a metal ball at a metal wall and have the wall bend. 4. Displaying the stress of each element by a different color. 5. An elastic rope that could stretch if pulled. Thanks for your insight!
OrenGL posted a topic in Math and PhysicsHi Everyone I'd like to learn more about FEM, in particular I'd like to write a little real-time 2D solver, perhaps a bending rod or some other deformable object. There is a lot of info out there on Google and plenty of books, but none seem geared towards video games (or real time visual simulation). Does anyone have a recommendation as to were to start? Regards, Oren
OrenGL replied to AntiGuy's topic in Math and PhysicsThe way I've seen collision for 3D plaformer characters done before is to use two spheres one on top of the other. The collision code is simpler (and faster). This also allows for shrinking the top sphere into the bottom one to support collision when the character crouches/balls-up/whatever.
OrenGL replied to Ultraseamus's topic in General and Gameplay ProgrammingSo at some point the program starts slowing down, but you can still debug it remotely? You should be able to take a profile snapshot when it starts out and compare it to when it slows down to hunt for clues. It will at least tell you where it is slowing down. Also another trick that works for me once in a while is to simply pause the app via Visual Studio. If you pause it 5 times (when it is slow) and it stops at the same place 4 times, you probably have a solid lead.
OrenGL replied to sephirath's topic in Math and PhysicsMy guess would be that you need to normalize the direction vector before you try get the Euler rotation from it.
OrenGL replied to DizzH's topic in Game Design and TheoryIf you're not that great on the technical or artistic side I would suggest trying to create a board game or a card game. A physical version that is! Let your friends/family/co-workers play it and get some feedback. I've worked with some designers on big budget games that still did this in their spare time.
OrenGL replied to sunandshadow's topic in Game Design and TheoryHave you played puzzle quest?
OrenGL replied to glPetter3f's topic in General and Gameplay ProgrammingQuote:Original post by glPetter3f Hi, the bug isn't some particular thing that doesn't work, everything becomes different, it is impossile to walk, all ennemies dissapear things become black and then the program crash or freeze. And eventhough some particular thing wouldn't work isn't it possible that the error is somewhere else in te code? I am wondering if there are any good debugging programs or anything like that? You're using visual studio 2003, that is a great tool for figuring out what is going on. When this happens, place a break point in an area you are familiar that is not working properly. For example if you are unable to walk, place a breakpoint in the part of code that makes your character walk. Investigate the state of things, you might not find the source of the problem immediately however you should be able to narrow down the list of possibilities. I'm assuming you are using version control? Since the game was running just fine for a while, you try binary searching for you change list in which the problem started. Check in what you have, and sync to an earlier version of your game. Test it, see if it happens and keep going until you find the problematic version. Then diff the changes and maybe you'll be able to eyeball the problem.
OrenGL replied to Dark Adept's topic in GDNet LoungeI'm in the same situation. If you're working 14hr days now, I don't think it's possible to do anything out side of work. You're probably readying this post on a Sunday evening at work in that case anyway... But once you're back to 9hr days, what I find best is getting up an hour early and heading out to a coffee shop. For me it's like "going to my other office" which keeps it productive. Certainly go before work (and do the gym after work). YMMV, if you need a desktop to compile, you might want to just to planning or brain storming out of home.
Would you mind posting the call stack?
OrenGL replied to Lykaios's topic in Graphics and GPU ProgrammingThe thing I would like the most is to be able to select the window I'm looking at by just looking at it and not needing to alt-tab or mouse clicking it. This is something that I need especially when working with multiple applications and keyboard short cuts. - Oren
Without looking at your code, the message: Access violation reading location 0x00000000. means that you are trying to dereference a null pointer. On the call stack you can usually go one frame up from where the program stopped to see the null variable.
OrenGL replied to BlackIgloo's topic in Artificial IntelligenceNavigation and real time combat would be the first IMO. From the list though it sounds like you can make this a non real time game if you prefer.


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Farmer of shot cattle is fuel bandit with €1.7m cab bill
A FARMER whose cattle were shot by Army marksmen this week has close links to Ireland's cross-border fuel smuggling trade and is facing a €1.7 million CAB bill.
A convicted fuel smuggler, John Hoey, had previously run an oil distribution company in Northern Ireland linked to the illicit cross-border trade.
Read our exclusive in this week's Sunday World.


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We felt welcome in this hotel from the second we arrived to the time we left. We were greeted with an umbrella and a smile on a rainy day. Roseletta at the front desk was one of the nicest people we met in Italy during our vacation. The hotel was built in the 60s and the decor still shows it even the brand new chairs in the restaurant. Call it dated or retro it's like going back in time and a nice departure from some of the new and polished hotels we are accustom to today. The rooms were clean and very comfortable. The breakfast was great and some one of the best we had all week. Our only regret was that we were not staying longer.
- Official Description (provided by the hotel):
- Tranquil Hotel set in cliffside enclave surrounded by trees, directly above hotel's private beach on the rugged coast far below. ... more less
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- TripAdvisor is proud to partner with Booking.com, Hotels.com, Expedia, Orbitz, Travelocity, Hotwire, TripOnline SA, Cancelon, getaroom.com and HotelQuickly so you can book your Excelsior Grand Hotel reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
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- Hotel Excelsior Grand


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Who's Hot, Who's Not, Pocono Edition
Rain wiped out practice and qualifying at Pocono Raceway on Friday, but the good news is the forecast for Sunday's Party in the Poconos 400 Presented by Walmart looks much better.
Here's who's hot and who's not headed into Sunday's race, the 14th event on the 2013 NASCAR Sprint Cup schedule. Rankings are based on average finish in the past five races.
1. CARL EDWARDS, 8.2 average finish — OK, this is just bizarre. Edwards is second in the Sprint Cup points standings, and has the best average finish of any Sprint Cup driver over the last five points races. But on Friday, Edwards acknowledged "systemic" communications problems at Roush Fenway Racing, where their cars lack the speed needed to win. So how is Edwards ranked No. 1? Easy — his worst finish in the past five races is 14th at Dover.
2. CLINT BOWYER, 9.0 — Like Edwards, Bowyer has been consistent rather than exceptionally fast. Bowyer's best finish in the last five races was second at Richmond, while his worst was 18th at Talladega.
3. KEVIN HARVICK, 11.0 — Happy time, people: Harvick won at Richmond and Charlotte, was fifth at Darlington and eighth at Dover. The one bummer was a 40th-place finish at Talladega.
4. JUAN PABLO MONTOYA, 11.4 — After a couple of lean years, the Colombian native is finally running well again, scoring a runner-up finish at Dover and a top five at Richmond.
5. JIMMIE JOHNSON, 12.0 — Had he not jumped the final restart at Dover, Johnson would be ranked much higher. Woulda', coulda', shoulda'. Didn't.
1. GREG BIFFLE, 26.2 — Expected to be a title contender, Biffle has finished 31st or worse in three of the last five points races.
2. BRAD KESELOWSKI, 24.2 — Boy, did Keselowski tank when his team had to deal with suspensions of their crew chief, car chief and team engineer. Keselowski went four races without finishing better than 15th.
3. JAMIE McMURRAY, 23.4 — It's been a struggle lately for McMurray, who has not finished higher than 16th in the last five races.
4. KYLE BUSCH, 21.8 — It's feast or famine for Busch. His last five finishes are: 24th, 37th, 6th, 38th and 4th.
5. RYAN NEWMAN, 19.8 — Ramming into David Gilliland and finishing 36th at Dover wasn't the kind of end to his day that Newman wanted.
FOX Sports Go


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Heavy duty tire for city or touring use. Excellent tire for when you can't justify the weight of the Schwalbe Marathon Plus. No slip grip compound for reliable traction and increased tread life. Extremely puncture resistant Protex belt. Folding bead keeps it lighter. 400D Lite Extra Cord technology increases side-cut resistance.
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This is my go to winter and touring tire.
It's lightweight, flat-resistant, grippy in the wet, fast on pavement, handles great on other surfaces like gravel. And pretty inexpensive.
These are not a high-mileage tire - probably have about 2000-2500 miles in 'em before it's time to replace.
After 2 yrs on a pair of these tires on smooth to rough roads, dirt, hard packed gravel, here are my thoughts surprisingly flat resistantflat proof (0 flats to date), very decent rolling resistance (but not like racing tires), and long wearing tread. They cost more than I like to typically spend for riding on crappier roads, but....worth it my view. The only tire I view as a better all-rounder is the Marathon Supreme which is every bit the Panaracer but smoother on chip seal and perhaps faster rolling overall. How these tires get used is more funchallenging 4-6 hr rides, 4000-8000', 40-60 miler, but not racing. I prioritize flat proof comfort speed. Lastly aside from the Conti 4000S (a nice fast tire that I would gladly use on nice roads which I don't have), the other Conti tourgravel type tires roll like rocks, indestructible, but rocks. I had been a Conti user for 10 yrs but no longer. Hope this helps you.
A follow-up to my review on 9152015. The (700c x 32mm) tire has a nice roll and feel and grips the asphalt nicely especially when pushing the down hills hard. Unfortunately it was a poor wearing tire for me, the rear tire lost all the center tread after only 1000 miles of hill training. I kept them inflated (several times a week) at the suggested top range of 95psi. This tire has a great spunky feel and never had any flats but I think I'll keep shopping (Biketiresdirect) for that perfect match. Oh well, the search goes on, ride safe & have fun.
These tires were super easy to mount on my Trek DS 8.4. I put them outside in the sun for several hours and was able to install just using my hands, no tools (some baby powder) were required with absolutely no struggle at all. It probably is overkill but I also installed Mr. Tuffy (blue) liners with these. A nice roll and feel, a great tire for those that do road (asphalt) hill fitness sprints and want a little more spunkiness to their workoutride.
The great postings here helped with my decision, thanks also to Bike Tires Direct with your great selection of products. Love the prices at BTD and their super turbo fast (extremely well packed) deliveries. I'll be back for more great products. Ride safe & have fun.
I got the 700x28 as a possible gravel tire. I ended up using them all Winter on my road bike. They performed nicely. I ran them at 85-90 psi and it makes for a very comfy ride. Chip seal? What chip seal?
Installed 32mm version for minimally loaded touring duty on a 2010 Specialized Tricross. Smooths out chip seal and rough roads (90 psi). Computer shows them to be just as fast as standard road tires.
As for durability, I'm concerned. On their first practice tour (fall in western PA), 60 miles in (all paved roads), I ended up with a cut near the treadsidewall junction on the rear tire. Patched inside of tire with a small square of HD tape, and have put on another 200 miles in that state.
i intend to tour the Great Allegheny Passage come spring, and I'm hoping this incident was just an anomaly. Maybe I can get in enough miles to be confident by then, but still unsure for now.
As for the install, they were tight, but nothing too difficult (like the road tubeless tires I've been destroying my thumbs on the past 5 years). I would say they feel just as smooth (at 90 psi) as a 28mm tubeless tire (at 85psi).
I have a touringcross bike and ride 90% on road and 10% off road. I want a go-anywhere tire that lasts and doesn't flat easily. That counts out every cross tire I know of, because they wear out quickly and puncture easily. I was a longtime fan of the Pasela foldable tires. I logged multiple thousands of miles on mixed gravel and paved roads and only had one flat. Then I experienced a couple cases where the sidewalls got damaged. I searched around for something tougher. I was thinking Schwalbe but then discovered the T-Serv Protex. They look just like the Pasela but with back rubber over-molded onto the sidewalls. I ordered a pair and have been riding them for two months. I have been riding in all conditions Drywet, off road, on gravel roads, and mixed sometimes crappy pavement. They're working great. The tread provides adequate traction off road, and the rounded profile is good for cornering on the road. The only downside is they have slightly higher rolling resistance than the Pasela.
Used this tire for year-round commuting in the Northwest. Handles great in the rain and varied terrain and has enough protection to save me from doing a flat repairtube replacement in the cold and dark. Might not be the fastest tire on the road but I'll save the speed for the drier summer months.
These are light weight and are great for commuting. I had one pinch flat during a tour, and the road was in a repair zone. The road was torn up pretty good and I hit an area at pretty high speed. I have at least 750 miles on them and they perform quite well.
Substituted these for defective tread-separating Continental Contacts that I ran for a couple years and loved them as the 32's were actually about 30's.
We've put maybe 750 miles these Pana's and they ride very well, and exhibit no problems. 32 is an actual 32. They seem to hold pressure very well.
Our new go-to tire.
We're seniors riding Trek 7300 at avg speeds of 14-15mph. Over 9000 miles my Cateye. Most of it recently.
Second set of these tires, they are not too heavy and have very good flat resistance for thousands of miles!
I bought a pair of these in 700-32 to replace a set of Michelin City tires that just seemed to keep growing in size until they rubbed the chain stays on my old Trek. The Panaracers hold their size very well, so they solved that problem. The bonus for me is their puncture resistance and long life. I'm doing an overhaul on my old bike and checked my mileage log. I have 1,915 miles on the T-Serve's and they are still in great shape. I will swap the front and rear and estimate they will last at least another 1,900 miles. I have had a total of one flat so far (knock on wood) and ride some pretty crappy roads and dodge the occasional broken bottle on the I-205 bike path, so I am very happy with their puncture resistance
Got the 700 X 32 to replace the stock SpeedMax Comp on 2013 Surly CrossCheck. My requirements were light weight, puncture-resistance, suitable for bike paths, asphalt, and the occasional gravel, and maintains grip when wet. Had 700 X 35 Marathon and Cross Town on the CC while looking for something else. Have always like Panaracers (Dart, Fire, Cross Town) so gave the T-Serv a try. No problem with the install (Stock Alex DA16 rims). Did not have to use levers. I am very happy with the T-Servs on my daily 10 mile round trip commute.
I use these tires in the 32 size for fully-loaded touring, mostly bikeways wmixed surfaces, including occasional dirt roads. I probably should have used the 35mm due to load (it's about 250lbs bike, gear, and rider) but the tires don't complain. I never have to think about these tires, silent and strong. I have had no flats with them so far (about 1100 miles, knock on wood). I also have them in 25mm size for winter road use and i don't notice any difference in the rolling resistance vs a road tire of similar width (michelin carbon) on my other bike. Mileage seems good too, as there's still some tread left in the rear, more left in the front, as i would expect. All-in-all, they work for me.
Only have a few miles on this so far (riding front wheel only) but it's noticeably faster than the ultra-slow marathon's I had on before. If it's as durable as others say, this is a commuter's steal.
I really like this tire for daily commuting over rough asphalt road, packed crushed rock, hard trail and concrete. 32 x 700c tire, pro hybrid style commuter (170 lb. load)
Performs well on wet roads and cornering, not the best for loose gravel/loamy soil. Also, a little taller than the average tire.
I've used these tires in 28 and 32 on mu single speed commuter. They last a long time (3,000 miles on the rear and longer on the front). They are supple and fast for commuter tires and they grip well in all conditions. They do flat out occasionally. For a long tour, I'd probably chose the 32s over fancier and more expensive options.
Poor wearing tire... rear was completely done under 1000 miles. Then a tendency to make flats after half worn. Overpriced. Revising my earlier reviews of this tire.. nothing I'd buy again at even half their cost.
These replaced continental comfort contacts on a surly disc trucker with 700c wheels, a bike i use primarily for commuting. The t-servs mounted easily with no flat spots (a problem i had with the contis), and provide a smooth fast ride. I don't ride this bike on the edge of the envelope, but for what its worth i have been happy with cornering and stopping performance. When these go i will most likely replace them with the same model.
Purchased these tires to replace worn stock tires on a cannondale quick 4 (fitness hybrid). These tires are as advertised. Smooth ride, absorbs shock, handle great. I am not in any race, so I can't comment on rolling resistance nor am I concerned. They show no signs of excessive wear to date and were easy to install on my rims. Would buy again.
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Moldavite large, top quality!
Moldavite occurred about 14.2 million years ago by a meteorite impact. These pieces were found on the only existing legal Moldavite pit.
Article number: MLG5
Availability: In stock
€132,50 Incl. tax
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Moldavite occurred about 14.2 million years ago by a meteorite impact.
Because of the enormous impact by a meteorite, the rocks and sand melted into glass. This glass, which is a mix of terrestrial and meteorite material, was spread by the force of the impact over a large distance. This' glass drops "are also called tektites.
Moldavite Tektite is the only one in the world with a beautiful green color and makes it unique in its kind! These pieces are found in 'Chlum nad Tender' Bohemia, Czech Republic.
No specifications found


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Today, I lost a bet with my friends. I had to go to the super market and buy a copy of 50 Shades of Grey along with a cucumber. The cashier was trying so hard not to laugh while ringing me up. FML
Supersize MenuYou wanted you know what the top of the flops of all time was, and now you know.
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Criminal Law
Criminal Law Questions? Ask a Criminal Lawyer.
I'm a licensed attorney and I believe I can provide some assistance. However, I need a point clarified first....
Are you looking to obtain the license?
Do you already have the license and you are now looking for employment?
I'm looking to obtain a license. I'm considering an elder care franchise but need to know if I would be ineligible for a license. Below is the statute that is referred to in the licensing requirements:
Texas Department of Aging and Disability ServicesCriminal Convictions Barring Facility LicensureRevision: 09-0
Thank you for the additional information. There is no need to respond to this post, I'll be back when I have some answers for you.
Thank you for your patience.
I researched the statute and I've got good news, your old DUI conviction is not a problem!
As you indicated, Chapter 99 deals with the Texas Department of Aging and Disability Service (DADS). Chapter 99.1 includes definitions and 99.2 discusses Convictions Barring Licensure.
Within 99.2, the statute lists convictions that "may" prevent you from obtaining a license to work in a facility providing care for the elderly. While it lists about 18 different offenses, DUI is NOT one of them. Additionally, even if it were listed, the statute indicates that a person "may" be denied a license. So, a person with a listed conviction could still argue for a license, perhaps based on the age of the conviction or that it was there only conviction.
The short answer is no. As to the long answer...
I think we can all agree that alcohol would not fall under the categories of "dangerous drug" or "controlled substance". That leaves "drugs" as the only possibility. While there are compelling reasons to consider alcohol a drug, I do not believe it applies here.
First, the Texas statutes dealing with drugs do not list alcohol. Additionally, it would have been quite easy for the legislature to add the word "alcohol" to the statute if they wanted it there. This simple addition would have made the statute clear and unambiguous on its face, as the legislature desires. Since it isn't there, the only logical conclusion is that they did NOT intend alcohol to be considered.
Also, assuming you read the entire list, you will surely agree that the listed charges are more serious than DUI; inducing gang membership, tampering with consumer products, leaving a child in a vehicle, stalking, dog fighting, compelling prostitution, to name a few.
And, even if I were incorrect, one could still argue that the "may deny" a license language still permits discretion. With the charge being relatively non-serious, just a misdemeanor, and so old, 8 to 10 years ago, I simply can't see you being denied over this.


text:
Unit 7 OKs security door system for schools
TOLONO — The Unit 7 school board unanimously voted this week to authorize the purchase of a security door entry system for all four of the district's schools.
The systems will include a button that visitors will push to trigger a phone in the office. Office personnel will be able to see who is at the door via a wall-mounted camera with a speaker. The building secretaries will be given a list of questions to ask visitors before they are allowed to enter.
Parents who come in to pick up students will stay in the office, and their children will come to them.
"So there's no unsupervised adult movement in the building," Superintendent Andrew Larson said. "It will be a bit of a culture change. But we want to create an environment that's going to be safer for our kids."
Larson said the systems, which are being bought from Tri-Color Locksmiths in Urbana, would cost between $21,000 and $24,000.
News-Gazette.com embraces discussion of both community and world issues. We welcome you to contribute your ideas, opinions and comments, but we ask that you avoid personal attacks, vulgarity and hate speech. We reserve the right to remove any comment at our discretion, and we will block repeat offenders' accounts. To post comments, you must first be a registered user, and your username will appear with any comment you post. Happy posting.


text:
Knobbed acrosome defect is associated with a region containing the genes
Male infertility is an increasing problem in all domestic species including man. Localization and identification of genes involved in defects causing male infertility provide valuable information of specific events in sperm development. Correct condensation of the sperm head and development of the acrosome are required for fertile sperm. In the Finnish Yorkshire pig population a knobbed acrosome defect (KAD) has been reported which appears to be of genetic origin. In previous studies we have shown that a large number of affected spermatozoa have a cystic swelling anterior to the apical part of the acrosome.
Characterization of the knobbed acrosome affected sperm revealed that both the acrosomal granules and chromatin are affected. This type of KAD appears to be a previously unknown and serious form of the defect. A genome wide scan with PorcineSNP60 Genotyping BeadChip defined the KAD associated region within 0.7 Mbp on porcine chromosome 15. Two genes,
In this study we identified two candidate genes for a severe defect affecting both the sperm acrosome and chromatin that causes infertility. One of these genes,
Male infertility is becoming increasingly prevalent partly due to environmental factors, but many defects in sperm development arise from a genetic cause. Problems in the production and maturation of sperm are the most common causes of male infertility resulting in low sperm numbers, morphologically abnormal sperm or low sperm motility
Mammalian spermatogenesis is a complex process, where diploid spermatogonia develop into haploid, highly specialized spermatozoa. Spermatogenesis includes many testis-specific processes that are controlled by complex regulatory mechanisms
Sperm development is known to be disrupted during spermiogenesis in several acrosomal defects; e.g. globozoospermia in humans, where spermatozoa lack an acrosome
Knobbed acrosome defect has been recently described in the Finnish Yorkshire pig population
A whole genome scan with microsatellite markers showed increased homozygosity in KA-affected boars in chromosomes 3, 8, 14 and 15
Microscopical analysis of the KAD
In the confocal laser scanning microscopy three-dimensional reconstructions of the spermatozoa with acrosomal granules indicated that the granules protruded on both sides and contained a vacuolum (Figure
Figure 1
Microscopical examination of the KA-defect
Microscopical examination of the KA-defect. A. Confocal laser scanning microscopy images of spermatozoa with acrosomal granule from two KA-affected boars. Yellow lines indicate the perpendicular planes of the 3d-reconstructions. Arrows indicate a vacuolum inside the granule. B. TEM analysis of an acrosomal granule. Two vacuoles are present and the apical part of the nucleus shows a Y-shape.
SNP quality measures
Based on all available SNPs and the method to estimate IBDs in the Plink software package (pi_hat) the average relatedness among cases and controls was 0.24 and 0.26, respectively. These levels of relatedness are typical in the studied Finnish Yorkshire pig population. The average sample call rate was 95%. There were 2815 SNPs that did not work for any of the samples analysed. Excluding these SNPs, the average SNP call rate was 0.9982 (s.d. = 0.007) and the average minor allele frequency was 0.25 (s.d. = 0.14). Overall, the dataset contained 9216 monomorphic SNPs. Observed distribution of P-values in the Hardy-Weinberg equilibrium test statistics did not differ from expectations. In total, 183 SNPs (excluding SNPs on the X-chromosome) had a P-value <1.0E-06 being lower than expected.
Genome wide association analysis
The association test was performed for 47055 SNPs. The Manhattan plot of the log10 based P-values is presented in Figure
Figure 2
Manhattan plot of the log10 based P-values across all chromosomes for the KAD in Finnish Yorkshire pig population
Manhattan plot of the log10 based P-values across all chromosomes for the KAD in Finnish Yorkshire pig population. The KA-affected haplotypes of marked KAD associated region supported the expected recessive mode of inheritance and had the lowest P-values.
Table 1
Genotype counts of cases and controls, nominal, and permutated P-values from the recessive model for the best KAD associated SNPs.
Position, bp
Genotypes cases
Genotypes controls
Recessive, P-value
Permutated P-value
Figure 3
Haplotype counts in KAD cases and controls on chromosome 15 (91861321-95156189 bp, Pig genome build 9)
Haplotype counts in KAD cases and controls on chromosome 15 (91861321-95156189 bp, Pig genome build 9). SNPs that are shared among KAD cases are marked in grey background and the SNPs with the smallest P-value from the recessive model are in bold face.
Additional file 1
The KAD associated region in the Finnish Yorkshire pig localized on porcine chromosome 15. The KAD associated homozygous region in the Finnish Yorkshire was identified in chromosome 15 between base pairs 93723216 and 94155055. A promising candidate gene
Click here for file
Candidate genes HECW2 and STK17b
The most promising candidate gene
Another gene within the KAD homozygous region was
Analysis of the porcine HECW2 gene
The expression pattern of different
Table 2
Primers used for sequencing of the candidate genes HECW2 and STK17b.
Position, bp
Length, bp
Forward primer
Reverse primer
The exons (based on human AB037722), position of the fragment within the
Sequencing of the porcine
Figure 4
Alignment of HECW2 protein sequences at the detected polymorphism positions in various species
Alignment of HECW2 protein sequences at the detected polymorphism positions in various species. The only difference between control (Yorkshire) and KA-affected (Akr) animals was a change from isoleucine to threonine (SNP). However, this change was also present in the human protein sequence. Furthermore, a three aa deletion was identified when compared with the pig reference sequence.
Analysis of STK17b mRNA
The sequenced testicular mRNA of porcine
While the results of homozygosity mapping of the KAD in a previous study
Within the associated region we identified and sequenced two candidate genes
Protein ubiquitination is one of the fundamental regulatory post-translational modifications controlling intracellular signalling events. Ubiquitin-proteosome-dependent proteolysis plays an important role in selectively degrading and recycling proteins in many basic cellular processes including spermatogenesis
In spermatogenesis ubiquitination is required for various processes; for example the replacement of the spermatids nuclear histones with protamines during spermatid elongation
Malfunction of components in ubiquitination system has been shown to be a cause of male infertility
In this study we demonstrate the exact KAD phenotype in mature sperm. In addition to the acrosome, the spermatid chromatin is also affected. We have located the homozygous region for KAD within 0.5 Mbp on porcine chromosome 15 containing two genes
Animal material
Experimental material included 14 Finnish Yorkshire boars affected with KAD and 21 control boars. All affected boars were clinically examined and shown to display symptoms typical of the syndrome, but no other abnormalities. Sperm from affected and control boars was collected and the DNA obtained following phenol/chloroform extraction. Samples were diluted to 100 ng/μl in TE-buffer and used as templates for PorcineSNP60 Genotyping BeadChip (Illumina). Genomic DNA was also used for sequencing of the
For microscopical examination representative semen samples from KA-affected and control boars were fixed in formaldehyde for confocal laser scanning and transmission electron microscopy analyses.
Confocal laser scanning microscopy
Spermatozoa were labeled with LIVE/DEAD Reduced Biohazard Viability kit (red, L23102, Invitrogen). The labelling protocol was in accordance with the recommendations of the manufacturer. In brief, 50 μl DMSO was added to one vial of fluorescent dye and thoroughly mixed to make a stock solution. Spermatozoa were suspended in PBS at approximately 1 × 106/ml. One μl of fluorescent dye was added to the suspension. After 30 min incubation at room temperature spermatozoa were washed and resuspended in 1 ml PBS twice. One drop of suspension was put on a Superfrost slide and coverslipped and subsequently analyzed on a BioRad MRC 1024 confocal laser scanning microscope. Three-dimensional reconstructions were preformed using Volocity LE free software (
Transmission electron microscopy (TEM)
Cells were fixed in 2.5% glutaraldehyde in PBS (pH 7.2) for 2 hours at 4°C. After washing, samples were postfixed in 1% OsO4 and 0.5% K-ferrocyanide in PBS for 2 hours, dehydrated with a graded series of acetone, and embedded in Spurr's mixture. Semithin sections were stained by 0.5% toluidine blue (pH 8.5) Areas of interest were trimmed out by comparing the cut surface of the blocks with the semithin sections. Ultrathin sections were cut by an RMC MT-7 ultramicrotome, stained with 2% uranylacetate and lead citrate and analyzed on Philips CM10 electron microscope.
For high throughput genotyping DNA samples were analyzed by PorcineSNP60 Genotyping BeadChip (Illumina Ltd, San Diego, USA) in the Institute for Molecular Medicine Finland (FIMM, Helsinki, Finland). The PorcineSNP60 BeadChip has recently been developed as an outcome of the porcine whole genome sequencing project
Expression profiling and sequencing of STK17b and HECW2
For sequencing the full-length mRNA of the candidate genes
Statistical analysis
A recessive mode of inheritance was tested for each SNP separately. The recessive model was selected because the pedigree of KA-affected boars suggested a recessive mode of inheritance and the low frequency of the defect in the Finnish Yorkshire pig population. In the recessive model, for each SNP the frequency of homozygote animals for the minor allele (or for the major allele) was compared to frequency of heterozygote and other homozygote animals between cases and controls. In order to correct for multiple testing a permutation procedure was adapted to create empirical genome-wide P-values. Association tests and permutation were carried out using the software package Plink
Authors' contributions
AS carried out the molecular genetics studies, sequence alignments and drafted the manuscript. PU performed the statistical analysis and participated in drafting the manuscript. SN participated in the microscopical studies and contributed to drafting of the manuscript (microscopical studies). SP carried out the microscopical studies. MA participated in the design and coordination of the study. JV participated in the design and helped to draft the manuscript. All authors read and approved the final manuscript.
Funding for this study was provided by the Finnish Ministry of Agriculture and Forestry (Makera). The assistance of Tiina Jaakkola and Tarja Hovivuori in DNA extraction and Päivi Lahermo (Institute for Molecular Medicine Finland, FIMM) in genotyping with PorcineSNP60 Genotyping BeadChip (Illumina) is greatly appreciated.
The relation of computer-based measures of sperm morphology and motility to male infertility
The relationship between semen evaluation methods and fertility in the bull
Infertility in the male dog
Testis-specific transcription mechanisms promoting male germ-cell differentiation
Chromatin remodelling and epigenetic features of germ cells
Regulation of gene expression in post-meiotic male germ cells: CREM-signalling pathways and male fertility
Analysis of sperm function in globozoospermia: implications for the mechanism of sperm-zona interaction
'Round head' sperm defect. Ultrastructural and meiotic segregation study
Homozygous mutation in SPATA16 is associated with male infertility in human globozoospermia
Fertility of stallions with abnormalities of the sperm acrosome
Ultrastructural features and pathogenesis of knobbed spermatozoa in a boar
Reduced fertility after artificial insemination in a ram with a high incidence of knobbed acrosomes
Genetic sperm defects
The knobbed acrosome defect in four closely related dogs
Infertile boars with knobbed and immotile short-tail sperm defects in the Finnish Yorkshire breed
Some disturbances of acrosomal development and function in human spermatozoa
The ultrastructure of a characteristic spermhead-defect in the boar: the SME-defect
The occurrence of a "SME"-defect-like abnormality in the head of spermatozoa from a Norwegian Landrace boar
Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro
A novel HECT-type E3 ubiquitin ligase, NEDL2, stabilizes p73 and enhances its transcriptional activity
Ubiquitin signals in the developing acrosome during spermatogenesis of rat testis: an immunoelectron microscopic study
Expression and localization of the deubiquitinating enzyme mUBPy in wobbler mouse testis during spermiogenesis
DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis
Regulation of the apoptosis-inducing kinase DRAK2 by cyclooxygenase-2 in colorectal cancer
Nuclear localization of the serine/threonine kinase DRAK2 is involved in UV-induced apoptosis
Ubiquitin-dependent proteolysis in mammalian spermatogenesis, fertilization, and sperm quality control: killing three birds with one stone
Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization
Participation of the sperm proteasome in human fertilization
Proteasomal interference prevents zona pellucida penetration and fertilization in mammals
Extracellular localization of proteasomes in human sperm
Localization of a proteasomal antigen in human spermatozoa: immunohistochemical electron microscopic study
Proteasome localization and ultrastructure of spermatozoa from patients with varicocele--immunoelectron microscopic study
Rnf19a, a ubiquitin protein ligase, and Psmc3, a component of the 26S proteasome, tether to the acrosome membranes and the head-tail coupling apparatus during rat spermatid development
Ubiquitin-specific protease activity of USP9Y, a male infertility gene on the Y chromosome
Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification
VCY2 protein interacts with the HECT domain of ubiquitin-protein ligase E3A
Specific aspects of the ubiquitin system in spermatogenesis
Transgenic rescue of ataxia mice reveals a male-specific sterility defect
Design of a high density SNP genotyping assay in the pig using SNPs identified and characterized by next generation sequencing technology
PLINK: a tool set for whole-genome association and population-based linkage analyses
Haploview: analysis and visualization of LD and haplotype maps


text:
borehole video inspection Articles
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Related terms for "borehole video inspection ": borehole articles , video inspection articles , borehole video articles
This article was originally published in the May 2009 issue of Geodriling International. Mike Deed, Managing Director of Geoquip Water Solutions Ltd, considers how the Laval R-Cam 1000 portable downhole video camera could revolutionise the borehole inspection industry. THE ability to see into a deep borehole has been an aspiration for well drillers, well owners, and geologists ...
Published in the April edition of the Waterwell Journal, this article looks at the problems caused by iron bacteria and iron oxide in wells and boreholes and how Boresaver, a specialist range of cleaning treatments, can solve those problems. Mineral scale and bacterial slime contamination are becoming an increasing problem in groundwater bores in America. Iron bacteria and iron oxide deposits are ...
Mike Deed, Managing Director of Geoquip Water Solutions, is the author of Talking Point in the October issue of Groundwater Engineering. He focuses on the huge benefits of installing a geothermal system and the importance ...
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Hand-crocheted openwork with medallion detail and pretty pearl ornament. Thumb cut-out. * This item is Free People private label and is exclusive to our web site and Free People stores.
Fishnet Crochet Fingerless Gloves styled by the FP Me community
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Goa board result www.gbshse.gov.in Goa HSSC 12th Result
Download Goa Board 12th Result HSSC Result 2017-2018
Goa Board of Secondary and Higher Secondary Education (GBSHSE) Latest update for the Secondary & Higher Secondary Results On May 2, 2017-2018. Press Notification.
Goa Board Students Can Check Their Result On 02.05.2017-2018. Result can also be located on Following websites. www.schools9.com , www.goaresults.nic.in , www.indiaresults.com .
How To Download 12th Goa board Result 2017-2018
All of the students can check & download their result from 02.05.2017-2018. Result will be published on given websites or Download 12th Goa board Result 2017-2018.
Students can also View Goa board 12th result via SMS and IVRS by writing the msg in following format :
SMS : IndiaResults.com — 5676750 , BSNL -56505, voda -56730
GOA12<space>roll no
RESULT<space>GOA12<space>SEAT NUMBER to 56263
IVRS : BSNL >125560 , Voda >56731.


text:
MAD Raw Silk Pocket Dress
MAD Raw Silk Pocket Dress
A functional yet elegant dress that you can wear year round and great for layering, designed in collaboration with MAD. Silk noil, 3/4 length drop-sleeves, and side pockets. Machine wash cold delicate, hang to dry Hand dyed and sewn in Minneapolis, MN Model is 5'9" and wears size S/M
Dedicated to bridging the gap between customer and maker, this Minneapolis boutique focuses on meaningful and lasting collaborations with small, emerging makers and artists. They keep their racks stocked with an impressive selection of influential brands like Kowtow, Ali Golden, First Rite, Miista, Oak, Hackwith Design House, Baggu and so much more.
Do you have questions about this product? We're here to help!


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nCircle, the leader in automated security and compliance auditing solutions, today announced as part of their Bellwether Metric series that the top 25% of nCircle Benchmark antivirus community participants update their antivirus definition files within 7.7 days or less. Antivirus definition age (AVDA) is a metric many organizations use as a leading indicator of endpoint security hygiene.
"Antivirus definition age is a simple, yet powerful metric," noted Jim Acquaviva, vice president of product strategy for nCircle. "It's a key indicator of the overall health of antivirus and endpoint security initiatives. If this bellwether metric is not meeting policies or goals, empirical evidence shows that the overall end-point security program is likely to need serious attention. AVDA is just one aspect of endpoint security hygiene, but if this metric is poor, chances are good other areas of the security infrastructure may need attention as well. It's one of the leading security performance management indicators."
Out of date virus definitions do not catch emerging viruses, increasing the potential for information leaks or breaches. Viruses are also associated with other IT metrics, including system down time, that translate directly to increased resource costs.
nCircle Benchmark's free antivirus Scorecard Pack measures eight key performance indicators and compares the results to the Benchmark community to analyze your antivirus and end point protection security posture. The free Scorecard Pack includes:
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nCircle Benchmark Community Bellwether Metrics are a group of simple, yet powerful measurements that indicate the overall health of a specific security discipline, and are designed to help security professionals focus their attention where it's needed most. In addition to antivirus and end point protection metrics, the nCircle Benchmark Community Bellwether Metrics program includes key metrics for vulnerability management, patch management, identity and access management and configuration compliance.
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text: The GER Classes S46, D56 and H88 (classified Classes D14, D15 and D16 by the London and North Eastern Railway) are three Classes of similar 4-4-0 Steam Locomotive designed by James Holden (S46 and D56) and A.J. Hill (H88) for the Great Eastern Railway. They were given the nickname Claud Hamilton after the first Engine of the Class. All of these Locomotives survived to preservation around England.


text:
A simple and very basic 35mm film camera with a very large viewfinder and a built in electronic flash and red-eye reduction LED. Made in China for Keystone.
- Type: Point and Shoot
- Film: 35mm
- Lens: 28mm f/9.5 (built in lens cover also shuts flash off when closed)
- Shutter Speed: Non-adjustable
- Focusing: Fixed
- Flash: Built-in (on off switch on camera front)
- Battery: single 1.5v AA
Basic Operation:
To the load the film, open the back of the camera by pushing up on the back release button. Extend the rewind lever and pull up until the film canister holder clears the film canister chamber.
second section of your page here.