text:
Emergency Update: Click here for information on our emergency response to drought and hunger in the Horn of Africa
International Medical Corps began its work in Kenya in 1998 following the deadly terrorist attacks on the US Embassy in Nairobi. Since then, International Medical Corps has responded to manmade and natural disasters in Kenya with critical lifesaving services as well as the long-term development challenges facing the country. Today, International Medical Corps' focus in Kenya is primarily on preventing and treating the effects of HIV/AIDS amongst the most at-risk populations in the Western part of the country where contraction rates are still above the national average, as well as on improving the nutritional status of mothers and children.
46 Million
Median Age
19.3 Years
Life Expectancy
63.8 Years
Fertility Rate
3.31 children per mother
Infant Mortality Rate
39.38 deaths/1000 live births
Family and Community Health
Nutrition and Food Security
Women's and Children's Health
Health Services
In Western Kenya, where HIV prevalence is two times higher than the national figures, International Medical Corps is working with the most at-risk populations for contracting and transmitting HIV/AIDS. International Medical Corps established drop-in centers that provide free HIV/AIDS prevention and care services. The centers offer HIV/AIDS education, family planning; condom promotion, demonstration, and distribution; counseling and psychosocial support; and referral services. The centers also organize support groups for those living with HIV/AIDS and train community leaders in HIV/AIDS prevention.
International Medical Corps also empowers adolescent girls and young women with the knowledge to keep themselves safe from HIV. This is done through a combination of HIV prevention activities that have successfully addressed risky sexual behaviors, HIV transmission and acquisition, and gender-based violence. By the end of the program. International Medical Corps is also studying the impact HIV self-testing and peer education can have on lowering transmission of HIV among female sex workers, while at the same time working to build the capacity of the county and national health management team in implementing science-based research and HIV programming.
In 2014, International Medical Corps has partnered with Cure Cervical Cancer to establish and scale up cervical cancer screening and treatment in 10 clinics in Kenya, including in the eight drop-in centers it created and at two women's prison clinics. The 12-month project intends to screen and provide treatment to 3,500 women for cervical cancer.
International Medical Corps is currently working in seven counties to improve maternal and child health through nutrition interventions. This involves supporting the Ministry of Health to implement High Impact Nutrition Interventions (HINI) as well working with local organizations and other sectors, such as agriculture and the National Drought Management Authority, to build capacity and advocate for greater investments in nutrition. The HINI efforts target child under five years old and pregnant and breastfeeding women and to aim to reduce child mortality by 30 percent.
As part of a consortium with the Kenya Red Cross and the local organization Community Action for Rural Development, International Medical Corps is reducing child stunting through better access to nutrition services. The program also advocates for stronger political commitment to reducing malnutrition and child stunting at the county level and strengthens existing information systems to better understand how nutrition service delivery is working in health facilities.
Kenya Capabilities Statement
DREAMS Newsletter, Vol. 1 - September 2016
SLIDESHOW: A TOUR THROUGH KENYA (click the arrow on the right to flip through photos)
For 30 years, International Medical Corps has worked to relieve the suffering of those impacted by war, natural disaster and disease by delivering vital health care services that focus on training, helping devastated populations return to self-reliance.


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text:
Tommy's Nyet (DOS)
Published by
Developed by
Critic Score
100 point score based on reviews from various critics.
User Score
5 point score based on user ratings.
DescriptionTommy's Nyet is a DOS Tetris clone for one to six players. The player uses the numeric keypad to position falling blocks in a pit. Blocks can be moved right or left and they can be rotated too. When the player completes a row of bricks across the pit with no gaps, that row disappears and all remaining bricks in the pit drop down. When blocks are moved left or right they are moved in half-brick increments which, if the block comes to rest in this position, leads to rows of blocks that can never be completed.
Each row that is completed scores one hundred points. Each falling block scores points for every level it falls before coming to rest. The number of points scored is either equal to the level of difficulty, if 'peek mode' is enabled, or three times the level of difficulty if 'peek mode' is not enabled. 'Peek mode' is a switchable feature that allows the player to see the shape of the next block. The game has nine levels of difficulty and maintains the top ten scores for each level.
Promo Images
There are no promo images for this game
Part of the Following Groups
- 10 Tons of Games: Mega Collection 1 (games included)
- Genre: Falling block puzzle
- Tetris variants
- Tommy's Games
User Reviews
There are no reviews for this game.
Critic Reviews
There are no critic reviews for this game.
There are currently no topics for this game.
There is no trivia on file for this game.
There are no game credits on file for this release of the game. Everything in MobyGames is contributable by users.


text:
Rigorously selected ingredients
Print advertisment created by Tif, Brazil for Gauden Bier, within the category: Alcoholic Drinks.
Rigorously selected ingredients
Creative Directors: Fhabyo Matesick, Thiago Biazetto
Art Director: Ivaldo Ferreira
Copywriter: Rafael Coradine
Photographer: Robert Kneschke
I have seen a pretty similar ad for fresh fish. A man goes and fights with a bear to get fresh fish. So sorry done.
And then GOD said...
Let there be creativity!!!
I love this concept... But the picture is quite weak.
i dont think these rigorously selected ingredients will make me choose this beer over Heineken, might be interesting if there was some a special
processsing of ingredients that giveth a unique taste or such. This idea might have worked on an exotic fruit -that grew next to active volcanos only- flavoured vodka maybe? nice try though.
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text:
On the coastal crook of a quiet Australian peninsula sits Cooktown, a small town with big plans. Last year, this town of 2,300 heard somethi... More
Giving voice to community radio More
Zeigler says treat them more like Silver Alerts More
Efforts to preserve radio's history should include college audio heritage — before it disappears More
A new eBook from Radio World International is now available
Capitalize on area talent to engage listeners More
The radio market is dynamic with plenty of projects and opportunities everywhere More
We begin the annual roundup of new broadcast gear More
Commission veteran still highlights consumer issues and "smart, targeted" regulation More
We were proud of our work with LPFM but deeply concerned about another acronym More
People News
She comes back as VP of Broadcast Sales
Nominations for Radio World International open until Sept. 4
Consultation paper issued to private FM radio broadcasters on digital transition
Comrex' Raul Hun showcases Opal, a multiuser, HD-quality IP audio gateway that connects by clicking a link, and the next-gen IP audio codec ACCESS NX that allows more innovative portable broadcasting. (Sponsored)
AM/FM are still available, but hard to find
On the coastal crook of a quiet Australian peninsula sits Cooktown, a small town with big plans. Last year, this town of 2,300 heard somethi...
Specialty station to air 5 a.m.–7 p.m.
Exhibitors at the 2017 NAB Show highlight some of the products on display at their booths. (Sponsored)
You can learn a lot from history
Giving voice to community radio
Zeigler says treat them more like Silver Alerts
Capitalize on area talent to engage listeners
Interconnectée, elle peut suivre les réactions des auditeurs en temps réel
Provided IP-networked consoles for France's July 14 celebrations
V-Soft report, commissioned by NAB, seeks to assess scope of challenge for radio
Tips from a professional on how to get the best out of a local shoot
For many in our business, ham radio remains close to their hearts


text:
The MaPP initiative seeks to contract a Technical Coordinator with project management and contract management experience to provide support to the North Vancouver Island (NVI) Sub-regional Implementation Team. Scope of work is related to implementation of the MaPP North Vancouver Island Marine Plan.
Term: ASAP to March 2016 with possibility of annual extensions to 2019.
Download: Contract Opportunity (PDF)
Contact: Fiona Kilburn firstname.lastname@example.com
Deadline: 5pm on Monday, November 30, 2015


text:
We will be staying near Seal Rock at the end of August for 5 days.
Would like some suggestions for places to eat and the best things to do in the area within a one hour drive.
We will be staying near Seal Rock at the end of August for 5 days.
Would like some suggestions for places to eat and the best things to do in the area within a one hour drive.
Newport is a few miles north and the largest town in the area. My favorite place in Newport is north of town a bit [Yaquina Head Lighthouse] -- lovely lighthouse, pretty views, beach with tidepools. There's a smaller lighthouse in town at the state park. There is an aquarium if you have youngsters. You can crab off the docks, walk along the old bayfront, drink beer at the Rogue brewery [south of the bay]. Our fave restaurant is Local Ocean Seafood; there is an Irish pub in the Nye Beach area that is good [sorry, do not know the name].
If you want to take a whale watching or fishing excursion, Depoe Bay is a good spot [north of Newport]. Tidal Raves is a good spot to eat there.
Yachats it the other direction -- small town that is kind of fun. The Luna Sea Fish House is funky, small, but good food. I believe there are other good restaurants there -- hopefully someone will pipe up.
The whole Cape Perpetual area is beautiful -- drive to the top and take the short walk for amazing views. the Sea Lion Caves is a private enterprise where you take an elevator down to a large cave that is full of sea lions. It feels kind of hokey and touristy at first blush, but, once you are in the cave, it's interesting [and rather smelly!]. They will tell you in the gift shop if the sea lions are "in." Heceta Head is another lighthouse near there.
Thanks - I had already viewed this link and found the history interesting.
Thanks for the information - I like the "local" response, as those who live in the area know the best places to eat / visit.
Favorite places to eat in Newport: Local Ocean Seafoods, Saffron Salmon, Panini Bakery, April's, Panache and Chowder Bowl. If you have an ample budget and enjoy inventive (and absolutely delicious) cuisine, you will love Restaurant Beck in the Whale Cove Inn just south of Depoe Bay. Chef-owner Justin Wills is a multiple-time James Beard nominee, and was just selected by Oregon's governor to represent the state (defending champion) in the National Seafood competition this summer in New Orleans.
Hi and welcome to the forums, GigiandPapa. Of the posters here, I'm the only one who actually *lives* in the general area of Seal Rock, although others do visit :-)
There isn't much in Seal Rock itself - it's not really a town as such. Over the many years I've lived in the area, businesses ebb and flow in Seal Rock. There are a couple of galleries that have been there for years, though, plus the espresso stand, LOL.
Newport is only about 15-20 minutes to the north on a fast, straight stretch of highway, and most people in this rural area dine and shop there due to the big selection of restaurants and stores. Waldport is just a few minutes to the south and has a few small restaurants.
Be sure to drive south to Heceta Head lighthouse (now open again!) , and you may also be interested in taking dune buggy rides around Florence. Florence is about the one hour drive time limit, but if you wanted to see Roosevelt elk, you could drive about a half hour farther south to Reedsport. A few miles inland on Hwy 38 is the Dean Creek elk viewing area. You have about an 80-100% chance of seeing elk there.
A very short inland drive from just north of Seal Rock at Ona Beach will take you to the old Ona Grange and give you a glimpse of some coastal farms and ranches in the area.
There are lots of things to do in Newport - two lighthouses, aquarium, marine science center, oyster farm and so on. You can also take a bay cruise.
Depoe Bay to the north is a kind of kitschy (somewhat tacky, in my opinion) smll seaside town. If you are interested in whale watching, stop by the new whale museum in town.
From that link in post #1:
"It was not until 1936 that this remote location was connected by bridges to neighboring cities and its true beauty could be enjoyed by all."
Actually, *not* true at all!
As early as 1893 there was a wagon road connecting Seal Rock to both South Beach (south of Newport) and a town called Lutgens, on the north shore of the Alsea Bay. You can still drive some of it today (dirt) , although most of it is blocked by private property.
Also, once the town of Waldport became firmly established there was a ferry crossing the bay to points north. This was replaced in 1936 with the construction of the old bridge, which itself was replaced in 1992. For more information about the history of the area, stop into the interpretive center at the south end of the Alsea Bay bridge :-)
For plenty more restaurants and thing to do - just enter the town name in the search box at the top right of any TripAdvisor page.
Thanks OregonJohn97388 for the list of restaurants - as we are eating out most of the time, this is a great resource for us.
Thanks Half-Brit - the local people can give information that isn't always accurate online. I especially like the reviews of the locals for restaurants.


text:
Question about 1992 Mitsubishi 3000GT
This problem just suddenly started happening. The car can run and be driven for several hours or sometimes only for a few seconds. It doesnt matter if you are idling or at freeway speed, the engine light goes on, the car starts to run rough and this clicking (like an electrical short) can be heard under the dashboard. I pulled the radio out and traced the sound to the area where the door alarm is located (ie. door is open when key is in the ignition). Do you have any ideas?
Posted by bonebone47 on
Could simply be your battery is about to die and the voltage is getting too low.
if the engine light is going on then take it to a mitsubishi dealer and they can read the stored error code.
Posted on Jun 23, 2009
SOURCE: I have freestanding Series 8 dishwasher. Lately during the filling cycle water hammer is occurring. How can this be resolved
a 6ya expert can help you resolve that issue over the phone in a minute or two.
best thing about this new service is that you are never placed on hold and get to talk to real repairmen in the US.
the service is completely free and covers almost anything you can think of (from cars to computers, handyman, and even drones).
click here to download the app (for users in the US for now) and get all the help you need.
Posted on Jan 02, 2017
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text: Write the text of your article here! animal story is a computer animated comedy film written and directed by roc produced by ardaman rock's. it will be release in theatre's december 4th, 2020. plot- when yogi bear and boo boo bear are captured and taken away from their owner pinocchio they need to return back to him and spend the christmas holiday with him and his mom. cast- dan as yogi bear. justin timberlake as boo boo bear. nathan lane as timon. ashton ketucher as duncan. frank as pinocchio. amy pohler as candy bear. brooklyn decker as courtney. production- the production the film will be on october 7th, 2020.


text:
Alpinetrek expert Hannes
"This product is no longer available."
This is either an older model or we can or will no longer reorder this product from the manufacturer.
Don't give up – we have some other alternatives for you to choose from:
Buff - Original Buff Yarn Dye Stripes
Attributes & features
- Suitable for:
- Design:
- Multi-function bandana
- Recommended use:
- Climbing; Mountaineering; Hiking; Mountain biking; Everyday
- Material:
- Microfiber (100% polyester)
- Size:
- one size
- Extras:
- double-sided; seam-free; Polygiene treated
- Item No.:
- 203-0335


text:
Today, I got sent home early because a large fire broke out at work. I was greeted by the sight of my cocktard of a "boyfriend" making out on my sofa with another woman. He actually had the audacity and brass balls to claim he thought she was me. FML
It's in the can!Hey, you uploaded your photo, and you're cute as a kitten!
InceptionYou read an FML that mentions a badge, and in return, you've been awarded a badge. A badge inside a badge.
Work is a 4-letter wordVoting on an FML in the "Work" category on a Monday morning between 8 and 9 a.m. How ironic.
MobilityYou are connected to FML via the mobile site or an app. How modern.
I moderated this!In "Moderate the FMLs", you voted Yes on a story that was subsequently published. Well done!
Up and coming moderatorIt's nice of you to help us sort out the submissions, using FML's moderate feature.
Who's the fairest of them all?This is now the third time you've changed your profile pic.
I agree, my mouse works.200 "I agree" votes is a good start.
The MixerYou like to live life randomly, and we salute you.
50 favoritesLove knows no boundaries. You've already added 50 FMLs to your favourites list!
JudgmentalYou have voted "You deserved it" over 100 times.
YDI MasterYou made your 500th "You deserved it" vote.
The thumb strikes backYou have left your thumbprint on 2500 comments.14%
42See, son, moderating FMLs is like a marathon.0%
A new thumbYou've used your thumb on 1000 comments.36%
One more and it's business timeYou've received 68 Hugs on your profile. Kinky.0%
The return of the thumbYou have thumbed 5000 comments.7%
Today, I discovered I have epilepsy. 10 years ago, I told my mother about my frequent fits of vertigo, deja vu, nausea, flashes of memory and strange sounds, smells, and images, coupled with an other-worldly feeling. I thought they were holy visions. So did she. FML
Today, my mom asked me, once again, if my girlfriend of almost a year is just a cover up for being gay. FML
Today, I got a call from my son's kindergarten teacher. Apparently my son asked a girl to marry him. After she said no, he stabbed her with a fork. FML
Today, I slipped and fell down the stairs, landing hard on my knee. Through my screams of agony, my mom accused me of exaggerating the pain, and said I was just being an attention seeker, before finally taking me to hospital. I was told my leg was broken. She refused to apologize. FML


text: General comment: Can you use git send-email to send the patches? I think I had problems applying those patches before, but now I'm finding it problematic to apply patches that are attachments. > +# Make a (dummy) Fedora image using md devices > +fedora-md1.img fedora-md2.img: guest-aux/make-fedora-img.pl \ I believe this will run the Perl script twice on updates. You need to use a stamp file, eg: fedora-md1.img fedora-md2.img: stamp-fedora-md stamp-fedora-md: guest-aux/make-fedora-img.pl [etc] [rules] The tests pass for me, so ACK. Rich. -- Richard Jones, Virtualization Group, Red Hat http://people.redhat.com/~rjones Read my programming blog: http://rwmj.wordpress.com Fedora now supports 80 OCaml packages (the OPEN alternative to F#) http://cocan.org/getting_started_with_ocaml_on_red_hat_and_fedora


text:
Date: 2015-05-25
Investigation into the transparency of Hydro One's billing practices and the timeliness and effectiveness of its process for responding to customer concerns
Ombudsman closes last Hydro One case - Oversight ends today (Press release)
Case summaries 2014-2015
Ombudsman no longer able to take Hydro One complaints (Press release)
Ombudsman urges corporate culture overhaul, ongoing oversight of Hydro One (Press release)
Press conference (video)
Facts and Highlights (accessible PDF)
Ombudsman's remarks - In the Dark webcast (accessible PDF)
Submission to the Standing Committee on Finance and Economic Affairs relating to Bill 91, Building Ontario Up Act (Budget Measures), 2015 (PDF)
Statement from Ontatio's Independent Legislative Officers (Press release)
Ombudsman received 10,000+ complaints about Hydro One (Press release)
Case summaries 2013-2014
Hydro One investigation draws historic number of complaints (The Watchdog e-newsletter)
Hydro One investigation update: Complaints to Ombudsman more than triple (Press release)
Ombudsman to investigate Hydro One: "Alarming" complaints about billing practices, customer service (Press release)
Press conference (video)
Ombudsman's remarks - Hydro One (accessible PDF)
System Refresh
Egregious Errors and Baffling Bills
Who Are You Going to Call? Customer Complaints
Post-launch Calls and Complaints
Customer Relations Centre Backlog
Technical Glitches
Defects Detected
Exceptions Prove The Rule
No Bills
Wrongly Addressed Bills
Never Billed
Estimated Bills
Poor Customer Service: A Symptom, Not the Disease
From A+ to Failing Grade – Systems versus People
Communications Gap
Keeping Outsiders in the Dark
Obstructing the Ombudsman
Obfuscating the Ontario Energy Board
Mollifying the Minister's Office
Befuddling the Board of Directors
Inside Crisis – Outside Calm
Light Bulb Moment: Too Late to Apologize?
An "Innocuous" $30-Million Error
Ombudsman Intervention
Reputation Rehabilitation Begins
Centering on Customers
Centering on Customers
Corrupted Communications Culture
Training Wreck
It's Alive!!! – System Implementation
Call Centre / Pressure Cooker
Call Quality Assurance, Please Hold
Surveying the Situation
Knowing the Real Score: Performance-Based Compensation
Lessons Unlearned
Sunny Days Are Here Again
Refund or Discredit
Unclear Bills
Confusing Complaints Process
The Power of Apology
Crossed Wires – Billing Resolution Duplication
Committing to Customer Commitments
That's Cold - Winter of Our Disconnect
Credit Collection – Owing an Apology
Relying on Technicalities – Customer Beware
It's a Little Dense - Density Classifications
General Disservice – General Service Billing
Two Years Too Late – Retroactive Charges
Giving Credit Where it is Due
Still Looking on the Bright Side
Hydro One, Inc
Considering customers
Communicating openly and transparently
Improving staff training and supports
Enhancing call intake and quality monitoring
Transforming corporate culture and governance
Improving customer service practices
Committing to customers
Improving collection practices
Clarifying classifications
Lessons learned
Progress reports
Government of Ontario
Considering customers
Communicating openly and transparently
Improving staff training and supports
Enhancing call intake and quality monitoring
Transforming corporate culture and governance
Improving customer service practices
Committing to customers
Improving collection practices
Clarifying classifications
Lessons learned
Progress reports
It's all rehearsed, it's all scripted… It's not the right way to do business for anyone, let alone a provincial utility.
(W)e're struggling with the sheer volume of complaints. Under normal circumstances, we have approximately 8-9 staff assigned to escalations. We currently have in the range of 20 and we're still struggling to answer a high % of the calls. […]


text:
After: Millie Ayala
"Her hair was safe and not dynamic," says Cutler. "We moved her into the twenty-first century." Holcombe cut off several inches, adding lots of layers and "texturizing" the hair (cutting it so there are no blunt edges) to add movement. Colorist Tim Suter livened Millie's too-dark locks with a rich chocolate hue. "The color reflects more light and adds warmth, so it looks softer against her skin," Cutler explains. "When I walked out of the salon, I was floating," Millie says. "It's lighter, more professional, more fun. And if I don't feel like blow-drying, I can just [style it with] product."
Best for: Any type of hair, except tight, frizzy curls; all face shapes, except for long faces.
Styling tips: Apply a styling product with hold (such as Aveda Phomollient Styling Foam, $19, aveda.com), then blow-dry away from face using a small to medium-sized round brush, says Heser. Dab a paste like Cutler Definition Cream ($25, dermstore.com) or Aveda Light Elements Texturizing Creme ($24, aveda.com) on ends and flip them out.
For the off-the-face style seen on page 1: Start by applying a styling product with hold, then blow-dry hair toward the face using a small to medium-sized round brush, says Heser. Using your fingers, brush the hair back and off the face. Smooth on a little Moroccan Oil Hair Treatment ($37.50, amazon.com) to control fly-aways and frizz, then finish with a light- to medium-hold hair spray.


text:
- Last login Nov 12, 14 @ 6:25 am
Official L.A Lakers thread
Lol the D. Howard in a Lakers uniform looks dope. My Laker fan side says yes, but my baskteball fan side says no. I just wish he changes his mind about staying in Orlando, hes never going to win it there.2 Weeks ago in Sports
Work Out Routine
Cardio for 20 minutes every other day, work out (lifting weights, push ups, squats, etc) every other day for 25. Thats at home. If I have time I go to the gym and run a couple of miles. But that doesnt really give any results because there is virtually no after burning if you dont do hours on end. Just drink lots of water. You might gain a bit of weight if you drink a lot, but water weight is easy to get rid of.2 Weeks ago in Sports
SSX Official Discussion Thread
If theyre as dope as SSX as SSX Tricky, I'm copping. The other ones kinda ruined it though.2 Weeks ago in Gaming
WWE '12 Official Discussion Thread
Yeah, I havent played them since the PS1/PS2 days, but if its tight, I might get it.2 Weeks ago in Gaming
What Video Game are You Playing
Portal 2. I'm gonna get into LA Noire after Duke Nukem.2 Weeks ago in Gaming
Call of Duty: Modern Warfare 3 Official Discussion Thread
Only reason I play is because if I dont, I'll be left out of the loop with my friends.2 Weeks ago in Gaming
Show Yo Crib!!!
Yeah, my crib will be ridiculed.2 Weeks ago in Visual Arts
Just a few drawings & paintings.
Looks trippy man, I like it! Are you studying art or are these just for fun?2 Weeks ago in Visual Arts
Cover of my album (mad trippy)
[Quote] LOL! I was about to post that. Looks dope2 Weeks ago in Visual Arts
Battlefield 3 Official Discussion Thread
This is a must buy.2 Weeks ago in Gaming
Madden 12 Official Discussion Thread
I through it was a joke that Hillis was on the cover until I googled it. At least if there really is a Madden curse, no one will be surprised that Hillis had a down year. Bad idea after one good year. But the hall of fame edition has Marshall Faulk, so thats dope. I still love my Rams and Madden 03 was really when I started liking Madden, although the 2K series was better in my opinion.2 Weeks ago in Gaming
Halo 4 Official Discussion Thread
this series went down the drain imo2 Weeks ago in Gaming
Duke Nukem Forever Official Discussion Thread
bump, week away2 Weeks ago in Gaming
Batman 3
[Quote] I stopped reading the rest of the posts, to say, THIS!2 Weeks ago in Entertainment
Rise of the Planet of the Apes - 2011
^^ What? You trippin. I was never really fan of the oldies or the movie they made like 10 years ago, but this looks dope.2 Weeks ago in Entertainment


text:
Within the context of a free trade zone along with food safety laws introduced by the European Union (EU), Georgia's dairy industry has the opportunity to become a competitive market. To achieve such progress, Georgian agribusiness requires capacity building in order to increase competiveness and efficiency of value chains and to attract investments for the upgrading of existing equipment, improvement of food safety and hiring of new employees in accordance with new EU standards.
FAO and the EBRD, with the Association of Milk Producers of Ukraine (AMP), have taken initiative to address the issues at stake with the implementation of the project "Improving food safety through capacity building in Georgia's dairy sector."
With the completion of the first phase, experts successfully conducted a technical and economic analysis of the dairy sector for a better understanding of bottlenecks within the industry in order propose recommendations for development and modernization.
Now, FAO with the EBRD and the AMP, are working to address the problems identified in the dairy sector through knowledge transfer including i) raising awareness of the new policy in the context of the free trade zone and ii) improving the standards of safety, hygiene and efficiency of dairy production.
At a glance:
• Georgia has a solid consumer base for dairy products but lacks the necessary technical knowledge for efficient production and farm management
• Not increasing investments in the sector could lead to production costs of high quality milk rising by 50-70 percent.
• The AMP has successful experience in the modernization of dairy farms for high level of safety and quality of milk in several countries across the Caucasus region
• With the expert knowledge from the partners, the project presents a platform for knowledge sharing and technical training
• The First National Dairy Congress and the Caucasion International Dairy Congress was held in Tbilisi in March 2016 garnering great interest within the dairy industry and gathering more than 200 participants – managers and specialists of the leading dairy farms, authorities, commercial companies, dairy companies, industry associations, international organizations, financial institutions and the media. Delegates from 10 countries took part in the successful forum. See more here: (links to press release, photo gallery, etc.)
• The team carried out theoretical and practical trainings on all technological aspects of milk production for a group of 20 managers and specialists of Georgia's dairy farms over the course of 10 intensive days of workshops on the farms. See more here: (links to photos etc.)
Project Leader:
Andriy Yarmak; email@example.com


text:
Correction - Ballarat Public Holiday Closure
Published: Monday 2nd November 2015
Please note that ACU's Ballarat campus will be not be closed this Friday due to a public holiday as advised last week's Bulletin. The correct date of the public holiday closure is next Friday 13 November.
The campus will be closed for one day on Friday 13 November due to the Ballarat Show public holiday.
Apologies for any inconvenience.


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We all know how to talk with our children about stranger danger, but child abuse, especially sexual abuse, is a difficult topic to discuss for most adults. It's uncomfortable to think about your child or a child you care for being harmed or mistreated in any way. But, when it comes to our kids, we have to step up to the plate, be aware of the signs and act responsibly. The fact of the matter is, sexual abuse is a reality and it happens every day. It's likely you know a child who has been or is being abused. Do you know what to do about it? As a parent, here's what you need to know about sexual abuse:
Step 1: Learn the facts
Step 2: Minimize Opportunity
Eliminate or reduce one adult to one child situations where your child is alone with another adult. You have the power and the right to protect your child.
Step 3: Talk about it
One of the best protections against abuse is our relationship with our children. Encourage open conversations with your children regularly. Only 29% of parents talk with their children about personal safety. Ask your child to unplug from their computer or phone for a few minutes and talk about the tough issues.
Step 4: Recognize the signs
Physical signs of abuse are not as common as emotional and behavioral signs. Look for:
Step 5: React Responsibly
If your child tells you someone has touched them inappropriately,
Commit to having open and honest dialogue with your children. Get out of your comfort zone and talk to them about personal safety. Your relationship will grow as a result and your children will be safer.
For information on how to talk with your children or to schedule our More than Stranger Danger workshop for your group, contact Family builders at 405.232.8226 or email email@example.com.


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It only takes a minute to get started. Fill out our uncomplicated sign up form and login to the website.
This step is elective but will seriously raise your chances of your profile getting noticed by other daters. Tell people about yourself, your interests and upload your best photo.
Use our website's cutting-edge search to locate members who match your desired criteria. Or just look through listings and locate daters that interest you.
Discovered someone who appeals to you? Send them a few words or just a wink and let it develop from there. With a little luck you'll be on your way to an amazing adventure.
It only takes a few minutes


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On Truth Telling and the Diagnosis of Alzheimer's Disease
Erde, Edmund L.
Nadal, Evan C.
Scholl, Theresa O.
Brody, Howard
Tomlinson, Tom
Bibliographic Citation
Journal of Family Practice. 1988 Apr; 26(4): 401-406.
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On Truth Telling and the Diagnosis of Alzheimer's Disease Erde, Edmund L.; Nadal, Evan C.; and Scholl, Theresa O. (1988-04)
Telling the Diagnosis of Alzheimer's Disease Markle, George B.; Drickamer, Margaret; Lachs, Mark (1993-03-11)
Family Members' Attitudes Toward Telling the Patient With Alzheimer's Disease Their Diagnosis Maguire, Conor P.; Kirby, Michael; Coen, Robert; Coakley, Davis; Lawlor, Brian A.; O'Neill, Desmond (1996-08)Advances in the accuracy of the diagnosis of Alzheimer's disease as well as progress in the genetics, aetiopathology, and therapeutics of the condition have stimulated a debate on whether patients should be informed of their ...


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Todd Hoffman hits rock bottom when, after seven seasons, a key crew member quits. Tony Beets transforms some rusty relics into a monster power barge, and a troubled Parker Schnabel misses his own birthday party.
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Seminar Hosting Guidelines-Institute for Software Research - Carnegie Mellon University
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Seminar Hosting Guidelines
Members of the ISR community frequently host external visitors who offer seminars to our faculty, staff, and students (as well as members of the broader community). This document is intended to provide some overall guidance around the hosting process.
Some seminars are part of series within the department (e.g., the COS Seminar Series), while others are ad hoc seminars developed by host faculty. Charges should be borne by the appropriate cost center (i.e., the program hosting the talk or the discretionary account of the faculty host). When in doubt, please contact the Business Office for clarification.
Room Reservations
Traditional room reservation channels should be used to secure space for all seminars.
Travel Arrangements
Travel arrangements for speakers, if necessary, should generally be made by the host's administrative support staff member. All travel expenses must comply with the university's Business and Travel Expense Policy.
The provision is refreshments is up to the host. We recommend the use of the campus catering service when light refreshments are required.
Seminar templates can be found
. Posters should be placed around the SCS complex at a minimum; work study students in the Business Office may be available to assist – check with Helen Higgins.
Email Announcements
Announcements may be sent to firstname.lastname@example.com, firstname.lastname@example.com, or to another mailing list as may be appropriate (e.g., email@example.com).
Website Updates
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- Anti-odor technology
- Ultra-durable rip-stop fabric
- Stretch-engineered waistband for mobility and comfort
- Offset belt loops for proper holster placement
The Under Armour Storm Covert Pants are built with an ultra-durable rip-stop fabric that is tough enough to put up with anything in the field! The UA Storm Covert pants also feature a stretch-engineered waistband that allows for superior mobility and insane comfort while having offset belt loops for proper holster placement.
Technical Information
Scent Control: Yes
Insulation: No
Fabric: 7.2oz Polyester
Waterproof: No; UA Storm DWR Water Resistant
Windproof: No
Number of Pockets: 6
Fit: Straight Leg, Loose
Leg Zippers: No


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Our Silver Supporters
To visit our supporters, click on each of their logos.
Supporters of Allergy & Anaphylaxis Australia
The work of Allergy & Anaphylaxis Australia is supported by many. Over the years we have worked with many Australian food manufacturers and they have listened to our concerns about ingredient (allergen) labelling and the limited choice of foods for those with food allergy.
Please be aware that Allergy & Anaphylaxis Australia does not endorse any product or food manufacturer. Always read and check the ingredient list on every product before the food allergic consumer eats the product. We hope these links increase your food choices and improve your shopping experience.
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Content updated February 2017


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If you poorness to acquire to romp metal guitar past you previously cognize it is a aggregation of theory, technique, and earthy talent. Everyone wishes to be the unsurpassable lead guitar player they can be. Everyone knows that you have to computer code the details such as as reading auditory communication and listening to auditory communication but supreme don't reason give or take a few the truly important holding that will set you obscure from other empire who poverty to swot up to comedy organize stringed instrument.
There are six arch tips that you will deprivation to recall.
- TechniquePost ads:
Classical and Fingerstyle Guitar Techniques
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- Position to Learn
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- Discipline
- Leadership Skills
Technique, of course, is one of the key weather you must computer address once you acquire to frisk metallic element guitar. It is genuinely consequential to get a smashing mentor to sea rover you the technique skills and to facilitate you to perfectible them. Many famous, and not so famous, metallic element guitarists will put in the picture you they ne'er finish learning, ne'er withdraw active and never die down perfecting their form. Playing stringed instrument is and look of your talent, you entail to procedure and unblemished your occupation.
Position to Learn
Most family don't realise that once you original enter a new phase to acquire to dramatic play front stringed instrument it is thoughtful to activity sitting fallen. This gives you more than more corner the market complete your instrument and allows you to compact on the effective music you are active. This power be bad-tempered for whatsoever but whether you prefer to sit or to remain standing you must e'er bring to mind to have some feet even on the flooring and to sit beside your put money on direct. This will assist you to fail to deal with hindmost strain or harm.
Hand Position
When you larn to dance atomic number 82 guitar you will soon agnize that one of the most meaningful belongings to learn is how to post your safekeeping. If you commencement from the emergence next to the true mitt positions you will breakthrough it by a long way easier latter to duck finished the disparate chords beside contentment. It also lets you move like greased lightning if one of your fella musicians has a challenge and makes a slip-up. At prototypic it will appear drastically ambitious mega if you have been mistreatment incorrect paw positions in the outgoing. Stick near it, activity manus positions regularly, it will pay off in the end once you healthy professional.
Attitude, and by this we don't imply having a bad attitude, is a terrifically distinguished piece to pay fuss to. Attitude affects your body expression and this is eventful once you acquire to dance atomic number 82 stringed instrument. Attitude and article prose go unneurotic to distribute you the trust you will requirement to execute as a metal guitar player. Watch self-made lead guitarists and see how they ever outer shell so considerably much upbeat than their back-up guitarists. That's attitude!
When you opening larn to dramatic composition pb guitar it is trouble-free to be disciplined. Your pilot anticipation makes it user-friendly to be able to spend hours annoying new auditory communication and techniques. As you modernize it is unproblematic to take possession of in to a flat that is respectable but i don't know not exceptional. It takes skill to hold a rank of convention that will unceasingly promote your skills. Discipline is what separates an standard lead guitar player from a superlative one. One bully tip is to compose out a programme and set a agenda near minute goals along the way to bread and butter you driven and on path.
Leadership skills
Leadership skills are esteemed to have if you want to be a atomic number 82 instrumentalist. You will be the one race gawk to for guidance, you will be at fault for all the musicians in the troop. You entail to be competent to pay focus to what every person is musical performance and be able to laminate mistakes that may arise during a musical composition. This may bear a time to get genuinely corking at but you should e'er be habituation yourself. Try to listen in while you are playing and you will amend. This tools will turn if you foundation accurate from the first once you cram to leap pb stringed instrument.


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Falkirk tie up Holden deal
By Mark Buckingham
Last Updated: 01/01/70 1:00am
Dean Holden has completed his move from Peterborough United to Falkirk.
Dean Holden has completed his move from Peterborough United to Falkirk.
Peterborough accepted an undisclosed bid for the defender over the weekend after Holden rejected an extension to his contract.
He agreed personal terms with Falkirk and passed a medical, with Posh director of football Barry Fry confirming the transfer.
"Dean Holden has agreed personal terms and completed his medical," Fry told the club's official website.
"I offered Dean a new contract several months ago. But he didn't sign - and after speaking to his agent, it was obvious he wanted to play at a higher level.
"We didn't want to stand in his way. He has been a good servant to this football club."
Falkirk boss John Hughes admits the capture of Holden ends a long pursuit of the former Bolton Wanderers stopper.
Hughes told his club's official website: "We have been tracking the player for some time and we are delighted to have concluded business with Peterborough United where I know he was very highly rated.
"He fits the bill as a player who is a real leader on and off the park and he comes to us at the peak of his career."


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Julian Assange says that Seth Rich, who Was MURDERED in DC, was the Wikileaks DNC Email leak Source!
On July 8, 2016, 27 year-old Democratic staffer Seth Conrad Rich who was murdered in Washington DC. The killer or killers took nothing from their victim, leaving behind his wallet, watch and phone.
Shortly after the killing social media were pursuing a "lead" saying that Rich was en route to the FBI the morning of his murder, apparently intending to speak to special agents about an "ongoing court case" possibly involving the Clinton family.
On Tuesday Wikileaks offered a $20,000 reward for information on the murder of DNC staffer Seth rich.
The List of KNOWN Clinton Murder Victims
1- James McDougal - Clintons convicted Whitewater partner died of an apparent heart attack, while in solitary confinement. He was a key witness in Ken Starr's investigation.
2 - Mary Mahoney - A former White House intern was murdered July 1997 at a Starbucks Coffee Shop in Georgetown .. The murder happened just after she was to go public with her story of sexual harassment in the White House.
4 - Ron Brown - Secretary of Commerce and former DNC Chairman. Reported to have died by impact in a plane crash. A pathologist close to the investigation reported that there was a hole in the top of Brown's skull resembling a gunshot wound. At the time of his death Brown was being investigated, and spoke publicly of his willingness to cut a deal with prosecutors. The rest of the people on the plane also died. A few days later the air Traffic controller committed suicide.
5 - C. Victor Raiser, II - Raiser, a major player in the Clinton fund raising organization died in a private plane crash in July 1992.
6 - Paul Tulley - Democratic National Committee Political Director found dead in a hotel room in Little Rock , September 1992. Described by Clinton as a "dear friend and trusted advisor".
7 - Ed Willey - Clinton fundraiser, found dead November 1993 deep in the woods in VA of a gunshot wound to the head. Ruled a suicide. Ed Willey died on the same day his wife Kathleen Willey claimed Bill Clinton groped her in the oval office in the White House. Ed Willey was involved in several Clinton fund raising events.
8 - Jerry Parks - Head of Clinton's gubernatorial security team in Little Rock .. Gunned down in his car at a deserted intersection outside Little Rock Park's son said his father was building a dossier on Clinton He allegedly threatened to reveal this information. After he died the files were mysteriously removed from his house.
9 - James Bunch - Died from a gunshot suicide. It was reported that he had a "Black Book" of people which contained names of influential people who visited prostitutes in Texas and Arkansas
10 - James Wilson - Was found dead in May 1993 from an apparent hanging suicide. He was reported to have ties to Whitewater..
11 - Kathy Ferguson - Ex-wife of Arkansas Trooper Danny Ferguson, was found dead in May 1994, in her living room with a gunshot to her head. It was ruled a suicide even though there were several packed suitcases, as if she were going somewhere. Danny Ferguson was a co-defendant along with Bill Clinton in the Paula Jones lawsuit Kathy Ferguson was a possible corroborating witness for Paula Jones.
12 - Bill Shelton - Arkansas State Trooper and fiancee of Kathy Ferguson. Critical of the suicide ruling of his fiancee, he was found dead in June, 1994 of a gunshot wound also ruled a suicide at the grave site of his fiancee.
13 - Gandy Baugh - Attorney for Clinton's friend Dan Lassater, died by jumping out a window of a tall building January, 1994. His client was a convicted drug distributor.
14 - Florence Martin - Accountant & sub-contractor for the CIA, was related to the Barry Seal, Mena, Arkansas, airport drug smuggling case. He died of three gunshot wounds.
15 - Suzanne Coleman - Reportedly had an affair with Clinton when he was Arkansas Attorney General. Died of a gunshot wound to the back of the head, ruled a suicide. Was pregnant at the time of her death.
16 - Paula Grober - Clinton's speech interpreter for the deaf from 1978 until her death December 9, 1992. She died in a one car accident.
17 - Danny Casolaro - Investigative reporter. Investigating MenaAirport and Arkansas Development Finance Authority. He slit his wrists, apparently, in the middle of his investigation.
18 - Paul Wilcher - Attorney investigating corruption at MenaAirport with Casolaro and the 1980 "October Surprise" was found dead on a toilet June 22, 1993, in his Washington DC apartment. Had delivered a report to Janet Reno 3 weeks before his death.
19 - Jon Parnell Walker - Whitewater investigator for Resolution Trust Corp. Jumped to his death from his Arlington , Virginia apartment balcony August 15, 1993. He was investigating the Morgan Guaranty scandal.
20 - Barbara Wise - Commerce Department staffer. Worked closely with Ron Brown and John Huang. Cause of death unknown. Died November 29, 1996. Her bruised, nude body was found locked in her office at the Department of Commerce.
21 - Charles Meissner - Assistant Secretary of Commerce who gave John Huang special security clearance, died shortly thereafter in a small plane crash.
22 - Dr. Stanley Heard - Chairman of the National Chiropractic Health Care Advisory Committee died with his attorney Steve Dickson in a small plane crash. Dr. Heard, in addition to serving on Clinton 's advisory council personally treated Clinton's mother, stepfather and brother.
23 - Barry Seal - Drug running TWA pilot out of Mena Arkansas, death was no accident.
24 - Johnny Lawhorn, Jr. - Mechanic, found a check made out to Bill Clinton in the trunk of a car left at his repair shop. He was found dead after his car had hit a utility pole.
25 - Stanley Huggins - Investigated Madison Guaranty. His death was a purported suicide and his report was never released.
26 - Hershell Friday - Attorney and Clinton fundraiser died March 1, 1994, when his plane exploded.
27 - Kevin Ives & Don Henry - Known as "The boys on the track" case. Reports say the boys may have stumbled upon the Mena Arkansas airport drug operation. A controversial case, the initial report of death said, due to falling asleep on railroad tracks. Later reports claim the 2 boys had been slain before being placed on the tracks. Many linked to the case died before their testimony could come before a Grand Jury.
28 - Keith Coney - Died when his motorcycle slammed into the back of a truck, 7/88.
29 - Keith McMaskle - Died, stabbed 113 times, Nov, 1988
30 - Gregory Collins - Died from a gunshot wound January 1989.
31 - Jeff Rhodes - He was shot, mutilated and found burned in a trash dump in April 1989.
32 - James Milan - Found decapitated. However, the Coroner ruled his death was due to natural causes".
33 - Jordan Kettleson - Was found shot to death in the front seat of his pickup truck in June 1990.
34 - Richard Winters - A suspect in the Ives/Henry deaths. He was killed in a set-up robbery July 1989.
35 - Major William S. Barkley, Jr.
36 - Captain Scott J . Reynolds
37 - Sgt. Brian Hanley
38 - Sgt. Tim Sabel
39 - Major General William Robertson
40 - Col. William Densberger
41 - Col. Robert Kelly
42 - Spec. Gary Rhodes
43 - Steve Willis
44 - Robert Williams
45 - Conway LeBleu
46 - Todd McKeehan
47 - 50 Benghazi 4


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Autumn 2009
Autumn 2009
International Pharmaceutical Industry 'Supporting the industry through communication'
Designing Biobetter Monoclonal Antibody Therapeutics By Glycoengineering IPI - International Pharmaceutical Industry
Leveraging Today's Technology for Clinical Trials Imaging
New Models for Cost Coverage of Drugs call for Improved Patient Compliance
Managing Pharmaceutical Globalization: A Work in Progress
%.&+/%+).&+##!)&,(!(*%&'%'(*%() !' *)'(&#.- .$&(* %&&+(+)*&$())"+)"
Contents EDITOR: Dr. Patricia Lobo Email: email@example.com DIRECTORS: Martin Wright Mark A. Barker
PUBLISHER: Clive Baigent EDITORIAL ASSISTANT: Linda Stewart Email: firstname.lastname@example.com BOOK MANAGER: Anthony Stewart Email: firstname.lastname@example.com BUSINESS DEVELOPMENT: Marie Powell, Chris Harradine Email: email@example.com DESIGN DIRECTOR: Anika Mistry
CIRCULATION MANAGER: Dorothy Brooks FINANCE DEPARTMENT: email@example.com RESEARCH & CIRCULATION: firstname.lastname@example.com
PRINTED BY: SW TWO UK www.swtwo.com
The opinions and views expressed by the authors in this magazine are not necessarily those of the Editor or the Publisher. Please note that although care is taken in preparation of this publication, the Editor and the Publisher are not responsible for opinions, views and inaccuracies in the articles. Great care is taken with regards to artwork supplied, the Publisher cannot be held responsible for any loss or damage incurred. This publication is protected by copyright. 2009 PHARMA PUBLICATIONS
Managing and Assuring Safety and Quality Throughout the Pharmaceutical, Biopharm, Medical Device and Healthcare Provider organisations, safety and quality remain at the top of the agenda driven by adverse events and the responses to them, especially when they are well covered in the media. The industry has come a long way over the past 20 years. Improvements have been driven by, for example the development and application of standards for pharmaceuticals and medical devices such as ISO 15378 and ISO 14971, and the creation of standards by regulators such as the NHSLA standards and Standards for Better in England. Mark Boult of DNV explains how the overall performance against the standards by the healthcare organisations has improved over the years. Göteborg – World Leader in Biomaterials Göteborg, on the west coast of Sweden, is the site of numerous breakthroughs in research and development within medicine, and the city is today a leading cluster in biomaterials and cell therapy. (IPI Market Review 2009) DRUG DISCOVERY, DEVELOPMENT & DELIVERY
COVER PHOTOS: In-House Productions
PUBLISHED BY: Pharma Publications www.ipimedia.com Diamond Key Building, Unit 4, Burwell Industrial Estate, Burwell Rd, London E10 7QG Tel: 0044(0)208 5917584 Fax: 001(480)247 5316 Email: email@example.com www.ipimedia.com All rights reserved. No part of this publication may be reproduced, duplicated, stored in any retrieval system or transmitted in any form by any means without prior written permission of the Publishers. The next issue of IPI will be published in autumn 2009 and quarterly thereafter. ISSN No. International Pharmaceutical Industry ISSN 1755-4578
Molecular Modelling for Optimization of Stabilized Therapeutic D-Peptides Peptides have become an important class of active therapeutic agents. In the last decade, a lot of synthetic peptide-based drugs entered the market as therapeutic agents. Dr. Udo Haberl & Prof. Dr. Hans-Georg Frank of Aplagen discusses Isosteric transformation which is a new and fast molecular modelling procedure enabling structure-based design of D-amino acid based peptides. It generates D-peptide based drug candidates in very short time. Biobridge-event 2009 Geneva: The Regenerative Medicine Conference Monday, 26th of October 2009 at the United Nations Office Geneva (UNOG), Palais des Nations, Switzerland, the Biobridge Foundation and its Scientific Committee will once again create a stimulating environment for debate and discussion on the topic of regenerative medicine, in which officials, scientific researchers, professionals and specialized scholars are invited to participate. It will take place under the Conseil d'Etat de la Republique et Canton de Genève high patronage. Big Pharma R&D—thinking big, acting small In order to mitigate the considerable risks in developing drugs, the pharmaceutical industry is going through an extended phase of consolidation to try to realise economies of scale. In this paper James Man & Andy Black of Kinapse outlines critical success factors for making disintegration work. This includes having talent management with a clear focus on leadership development, structuring operating units around clear areas of competence and performance, having clear divisions of accountabilities and transparent cost accounting between operating units.
CONSTANT CLIMATE FOR YOUR CARGO – WORLDWIDE: SWISS °CELSIUS –If your cargo needs to be kept warm or cool, Swiss °Celsius is made for you – our quality product for freight shipments, which are sensitive to temperatures and critical in terms of time factors. With temperature-controlled transport between -20 °C and +20 °C in special containers, we deliver your cargo to over 70 destinations all around the world. We care for your cargo.
Contents 38
Designing Biobetter Monoclonal Antibody Therapeutics By Glycoengineering Monoclonal antibodies (MAbs) currently comprise the fastest growing class of protein therapeutics - primarily for the treatment of cancers and autoimmune, infectious and inflammatory diseases. One area of great interest to developers, copiers and improvers of therapeutic antibodies is glycosylation since it can significantly influence the safety and efficacy profiles of the drug in this article, By Dr. Claire Morgan and Dr. Daryl Fernandes of Ludger shows how both the original drug manufacturers and the designers of follow-on biologics could produce biobetter antibodies through glycoengineering. In vitro alternatives to reduce animal use and to decrease drug development costs In preclinical drug development in vitro studies have obtained a more prominent position. Because of ethical considerations an increasing number of in vivo tests have been replaced by in vitro experiments. These tests are recommended by many organizations. The National Institutes of Health (NIH) and other federal agencies are committed to the welfare of research animals. These animals are protected by law, regulations and policies to ensure use in the smallest number possible and with the greatest commitment to their comfort. By: Ida A.J. Verbaan-Giebelen of NOTOX B.V. High-Throughput Antioxidant Determination – The ORAC Assay performed on Multidetection Microplate Readers Antioxidants, present in fruits, vegetables and whole grains, act as free radical scavengers and thus are thought to protect living cells from being damaged by reactive oxygen species (ROS). In this article Franka Ganske, Marjan Orban, and Michael Fejtl, of BMG LABTECH, presents s the ORAC assay as a tool to measure antioxidant capacity in fruit juices in a highthroughput microplate format.
Leveraging Today's Technology for Clinical Trial Imaging Over the last decade, medical imaging has been playing a greater role in the diagnosis of disease and, logically, there has been an analogous increase in the use of imaging in all phases of clinical trials to evaluate new drug candidates. Leveraging telecommunication tools for file transfer and integrating imaging with electronic data capture (EDC) has the potential to transform the manner in which imaging is used in clinical trials. Abraham Gutman of AG Mednet & Robert Quinn of Phase Forward analyses new tools that are now available that make this integration a reality and could provide better transparency for sponsors, improve data quality, reduce costs, give more de-identification options, and even reduce a firm's carbon footprint.
Central Component of Drug Development Contemporary lifecycle drug development encompasses in silico computer simulation modeling of drug molecule binding to both target and off-target receptors, nonclinical investigation, preapproval clinical studies, and postmarketing surveillance.1 Since cardiac and cardiovascular adverse drug reactions are serious and potentially lethal, their lifecycle assessment is critical.2 While assessment in all of these four phases is important, this paper focuses registration studies. J. Rick Turner, Senior Director, Cardiac Safety of Quintiles ECG Services has provided a sample of cardiac and cardiovascular safety assessments during contemporary drug development. An Interview with Kenneth G. Faulkner and James Paskavitz of Perceptive Informatics on a robust medical imaging methodology for Central Nervous System (CNS) clinical trials involving Alzheimer's disease India – Worlds best choice for clinical trials India has the potential to attract 5-10% of the global contract research outsourced market (all services including chemistry, toxicology and clinical research) over the next 5 years. The Indian government has recognized that India can become a hub for conducting global clinical trials. The Planning Commission's Report of the High Level Group on Services Sector highlights the opportunities and identifies the gaps that need to be addressed by the government for this sector. Dr. Rajam Jaishanker of Quest Life Sciences discusses the factors contributing to a gradual but steady growth. Ethics Committees in India- An insight Ethics Committee (EC(s)) constitutes the third arm of the research triangle and it can make significant contribution towards the ethical conduct of clinical trials. Post 2005, i.e. after schedule Y has been amended, obtaining ethics committee approval is one of the mandatory step towards conduct of the trial. While amended Schedule Y1, ICHGCP Guidelines2, Indian GCP3 specifies standards to be maintained by ECs, the majority of institutions in India still lack compliance with the applicable standards in a true sense. Deepti Goel of Cliniminds takes a look at major areas that require immediate attention and correction. LABS & LOGISTICS
Successfully involving central laboratories: How to avoid fundamental errors No doubt, central laboratory services are complex, particularly due to the logistical tasks involved. One aspect renders central lab services different to almost any other service outsourced by Pharma or Biotech companies during a clinical trial: biological samples have a limited stability and if the lab provider does not take this into account in many cases the samples may
Cardiac and Cardiovascular Safety Assessment: A 4 INTERNATIONAL PHARMACEUTICAL INDUSTRY
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When buying a glass piece, there are lots of important factors to consider. Oddly, one of the most important is the most overlooked; glass thickness and quality. Let's talk about glass quality first…
Glass Quality
Glass quality is important in the ways of clarity and strength. The higher quality of glass, the stronger and more pristine the glass will be. Glass quality is determined by the material of glass, and the skill level of the glass blower. Most brand name pieces, and those made in the USA, will be made of high quality borosilicate glass (the material to make lab equipment, also called "German schott" or "schott duran); while certain tubes at your local head shop are most likely made of low quality Chinese- glass. The higher quality glass will be much more durable than the lower. The skill level of the blower can be determined by simply looking at the piece. Clean joint welds are signs of talented glass blowers.
Glass Thickness
Glass thickness affects the weight and durability. Glass thickness is counted in millimeters, usually ranging from 3mm to 9mm, with 9mm being the thicker of the two. Thicker glass means a more durable, higher weight piece. The heaviness of the glass is more of a personal preference, but heavier pieces do have a sense of quality to them.
If it comes down to it, it's better to have higher quality glass than thicker glass. A piece could be 9mm thick, but if the glass is poor quality, it's more likely to break than a 4mm thick piece with higher quality glass. Luckily, Smoke Cartel carries a great selection of pieces for a great price, so you don't have to compromise.


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1 results containing "Bobo, Thomas."
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[Private Thomas Bobo of H Company, 5th South Carolina Infantry Regiment; H Company, 9th South Carolina Infantry Regiment; K Company,1st South Carolina Light Artillery Regiment in uniform and hat decorated with flowers]
[between 1861 and 1865] | 1 photograph
AMB/TIN no. 2801 [P&P] | LC-DIG-ppmsca-32122 (digital file from original item)
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Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels
Postnatal endothelial progenitor cells (EPCs) have been successfully isolated from whole bone marrow, blood and the walls of conduit vessels. They can, therefore, be classified into circulating and resident progenitor cells. The differentiation capacity of resident lung endothelial progenitor cells from mouse has not been evaluated.
In an attempt to isolate differentiated mature endothelial cells from mouse lung we found that the lung contains EPCs with a high vasculogenic capacity and capability of
Mouse lung microvascular endothelial cells (MLMVECs) were isolated by selection of CD31+ cells. Whereas the majority of the CD31+ cells did not divide, some scattered cells started to proliferate giving rise to large colonies (> 3000 cells/colony). These highly dividing cells possess the capacity to integrate into various types of vessels including blood and lymph vessels unveiling the existence of local microvascular endothelial progenitor cells (LMEPCs) in adult mouse lung. EPCs could be amplified > passage 30 and still expressed panendothelial markers as well as the progenitor cell antigens, but not antigens for immune cells and hematopoietic stem cells. A high percentage of these cells are also positive for Lyve1, Prox1, podoplanin and VEGFR-3 indicating that a considerabe fraction of the cells are committed to develop lymphatic endothelium. Clonogenic highly proliferating cells from limiting dilution assays were also bipotent. Combined
The lung contains large numbers of EPCs that display commitment for both types of vessels, suggesting that lung blood and lymphatic endothelial cells are derived from a single progenitor cell.
In the developing embryo blood vessels and later also lymphatic vessels are formed via an initial process of vasculogenesis. This is followed by sprouting and intussusceptive growth of the vessels, termed angiogenesis for blood vessels and lymphangiogenesis for lymph vessels. These mechanisms give rise to a complete blood and lymphvascular system consisting of arteries, veins, capillaries and collectors. Endothelial cells (ECs) are specified according to the circulatory system (blood
More recently it has been demonstrated that preparations of human large vessel endothelial cells contain a moderate number of cells that have a higher single-cell-layer growth potential and show endothelial colony-forming activity
In recent years, we have established numerous endothelial cell lines from different mouse strains and immortalized them by transduction with polyoma middle T (PmT) virus
Mouse lungs contain ECs with high proliferation capacity
Using adult mouse lung for magneto-bead selection of CD31+ endothelial cells, we discovered that the lung contains endothelial cells, which have a high proliferative capacity. When lungs from three animals were combined and used for tissue homogenisation followed by anti-CD31 cell sorting, only very few isolated cells started to proliferate (Figure
Figure 1
Cultured mouse lung EPCs at different time points after isolation
Cultured mouse lung EPCs at different time points after isolation. Three lungs were used for magneto bead isolation and cells were plated in one well of a 12- or 6-well plate. a) One day after cell isolation, the inset in a) shows a small colony with high replication capacity. b) Homogeneous large colonies with high proliferation capacity have formed six days after isolation. c) Lung EPCs in passage 12 and d) in passage 31. Scale bar = 200 μm.
Proliferation of mouse lung EPCs was compared with endothelioma cells isolated from a cognate mouse strain over a six-day period. After a two-day lag phase, the number of EPCs was 3.2-fold higher than that of endothelioma cells on day 4. The significant difference in cell growth was also present after 6 days (Figure
Figure 2
Mouse lung EPCs show a high proliferation capacity if compared with mouse endothelioma cells derived from the same mouse strain
Mouse lung EPCs show a high proliferation capacity if compared with mouse endothelioma cells derived from the same mouse strain. Serum-stimulated growth was compared in equivalently seeded EPCs and endothelioma cells over a 6-day period. After a lag phase, a significantly increased growth rate appears in EPCs compared to endothelioma cells.
A key feature of stem cells is their ability to maintain stem cell properties after division, known as self-renewal. To exclude the possibility of overestimation due to cell aggregation or formation of secondary colonies, a single-cell clonogenic assay was performed by culturing single cells by limiting dilution in individual wells of 96-well plates. We found that ~15% (± 3.6%) of the wells contained highly proliferating colonies. These wells were 80-100% confluent after 18-20 days and had a cell number of 4.3-104 (± 1.4 × 104) cells/well at the end of the experiment (day 28). From the highly proliferating clonal MLMVECs six lines were established and maintained in culture for several passages. Clones were cryopreserved at passage 15 and 20. From these cultures four clones were further characterized for BEC (blood endothelial cell) and LEC (lymph endothelial cell) markers (see below). The abundance of endothelial precursor cells within the MLMVEC population provides an explanation for the accelerated growth of MLMVECs as compared with mouse endothelioma cells.
Mouse lung primary EPCs in early culture are heterogeneous and have the potential to differentiate into lymphatic and blood endothelial cells
In one of the earliest reports on the isolation of human BECs and LECs from a dermal cell suspension, it has been shown that the cells form separate homotypic clusters
Figure 3
Heterogeneity of mouse EPCs immediately after isolation and EPC subclones are bipotent
Heterogeneity of mouse EPCs immediately after isolation and EPC subclones are bipotent. A-B) CD31+ BECs surround LECs, which are in the centre of a growing colony and which are Lyve1 positive. BECs are more positive for CD31 than LECs and all LECs specifically express the transcription factor Prox1. C-D) After a single-cell clonogenic assay all cells from two representative subclones (D5 and B3) are positive for CD31, a panendothelial marker. Only cells differentiated into lymph endothelial cells (LECs) are Lyve1 or podoplanin positive. 150-200-fold.
To study the hypothesis if a dual differentiation capacity can be found in endothelial precursor cells, a single-cell clonogenic assay was performed. Four subclones derived from a single-cell suspension were used to study the expression of CD31, Lyve1, Podoplanin and Prox1. The results showed very clearly, that both endothelial cell types could be found in each subclone, indicating that the precursor cells are bipotent. A representative result is shown in Figure
Additional file 1
Heterogeneity and pattern formation of mouse EPCs after isolation and CD31 cell sorting shown at lower magnification. a) Pattern formation of almost confluent cells after the first CD31 selection shown by phase contrast microscopy. b) The same cells as in a). Heterogeneity of cells is demonstrated by immunofluorescence several days after Lyve1-positive selection. Note CD31-positive BECs surrounding Lyve1-positive LECs, which express variable amounts of CD31 (yellow). 100-fold.
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We also observed that isolated mouse lung endothelial cells were either Lyve1+ or Lyve1- and that the majority seems to represent LECs and the minority BECs (additional file
Additional file 2
Isolation of MLMVECs from mouse lungs by FACS sorting. Low passage (passage 3) MLMVECs were sorted by FACS into CD31+/Lyve1+ (gate R1) and CD31+/Lyve1- (gate R2) cells for further subculturing and immunophenotyping. The percentage of cells in the different regions is indicated (%).
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Additional file 3
Subculturing of Lyve1+ and Lyve1- cells after CD31/Lyve1 cell sorting. Early passage MLMVEC (passage 3) were FACS sorted (same cells as in supplementary figure
Click here for file
Mouse lung EPCs are capable of vascular sprout formation
One of the key characteristics of endothelial cells is the intrinsic capability to form vascular networks on Matrigel
Figure 4
Mouse EPCs from lung exhibit rapid
Mouse EPCs from lung exhibit rapid
Additional file 4
Sprout formation of MLMVEC spheroids on Matrigel in the presence of different growth factor combinations. Quantitative sprout formation of MLMVECs (in μm) in the presence of different growth factors 5 days after plating together with Matrigel. Growth factor concentrations in Matrigel: VEGF-C (500 ng/ml); VEGF-A (50 ng/ml); bFGF (20 ng/ml); TNF-α (10 ng/ml); HGF (20 ng/ml); ECGF= endothelial cell growth factor supplement (50 μg/ml + heparin). Sprouts were measured with an inverted stereo microscope (Zeiss) using specific software (AxioVision Rel. 4.5) for the determination of sprout lengths. Cells from A were isolated from C57Bl/6 mice and cells from B were isolated from Balb/c mice. Values= means ± SD.
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Characterization of mouse lung EPC and endothelial markers
The use of cell surface markers for the characterization of cell type specificity has greatly improved the phenotyping of mouse endothelial cells, because these change their morphology depending on passage number and rate of confluence. In this study, we sought to determine whether mouse EPCs express panendothelial markers as well as markers for progenitor cells and for BECs and LECs. The key endothelial marker, besides cobblestone morphology, is CD31 (PECAM-1). Only cells from colonies were used in this study, which were more than 90% positive for this marker. Some cells from early passages were CD31 FACS sorted again if necessary. Routine FACS analyses between passage 4-8 indicated that more than 99% (99,5 ± 0.2%) of MLMVECs were CD31+. Other panendothelial markers include CD102 (ICAM-2) and CD105 (endoglin), which are also expressed in EPCs (Figure
Figure 5
Mouse lung EPCs retain a microvascular endothelial phenotype
Mouse lung EPCs retain a microvascular endothelial phenotype. Immunophenotyping of cells was performed with flow cytometric analyses. Mouse lung EPCs express the pan-endothelial markers CD31, CD102 and CD105. They also express circulating EPC markers CD34, CD133, vascular endothelial growth factor receptors 2 and 3 (VEGFR-2 + VEGFR-3). The new marker LA5 and MECA32/PV1 for blood endothelial cells (BEC) is also expressed as well as the lymphatic markers Lyve1 and podoplanin. The pan-lymphocyte marker CD45 was not detectable.
Additional file 5
Double labelling of lung EPCs with Lyve1, MECA32 and LA5. Immunophenotyping of cells was performed with cytometric analysis. Mouse lung EPCs are double positive for Lyve1 and MECA32. Only one part of the Lyve1+ cells are also positive for LA5. Cells from two isolations have an almost an identical pattern of the three markers.
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A very classical marker for vascular endothelial cell is eNOS or its product NO (nitric oxide). Here we have used the cell permeable dye DAF-2 (see methods) which is relatively non-fluorescent. However, in the presence of NO the derivate is converted to the fluorescent derivate as show by FACS analysis indicating, that the cells produce NO (additional file
Additional file 6
Mouse lung EPCs have the capacity to produce NO in vitro. Fluorescence detection of NO production was done with a practical FACS assay with living cells. Membrane-permeable DAF-2 diacetate has been used for indirect detection of NO production.
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Mouse EPCs are not transformed
Based on the high proliferation rate and the high vasculogenic capacity under culture conditions, we investigated the possibility that these cells might be transformed and display a malignant phenotype. Therefore, we determined anchorage-independent cell growth in a 0.8% agar matrix. As a control, a mouse beta tumor cell line (βTC) was used in parallel with the same number of cells per well. Mouse EPCs did not display proliferative capacity, and no clusters of cells were found throughout the agar (Figure
Figure 6
Mouse EPCs do not display evidence of transformation
Mouse EPCs do not display evidence of transformation. The evaluation of anchorage-independent growth was examined in mouse EPCs and cells derived from a beta cell tumour seeded onto a soft agar matrix. Whereas EPCs did not grow on soft agar, beta tumour cells (used as control) formed frequent colonies during the 8 days of cultivation. 50-fold.
In vivo vessel formation of mouse EPCs in Matrigel implants
One of the key questions to be answered was whether mouse EPCs possess the ability to generate capillaries and larger blood and lymphatic vessels
Figure 7
In vivo studies of mouse lung EPCs after lentiviral transduction with GFP marker gene
In vivo studies of mouse lung EPCs after lentiviral transduction with GFP marker gene. A) Cells as a monolayer (left) or as spheroids (right) used for Matrigel implantation into mice. B)
Additional file 7
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Additional file 8
In vivo studies of mouse lung EPCs to detect blood and lymphatic networks in the implanted Matrigel. Formation of blood and lymphatic networks in the same Matrigel by triple staining with anti-GFP, anti-podoplanin (LEC-specific) and anti-CD31. Note that the merged figure contained GFP-tube forming cells which are CD31 and podoplanin positive (LEC) and podoplanin negative cells (BEC). 200-fold.
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Endothelial precursor cells are found in different niches
Bone marrow as well as peripheral blood of adults and umbilical cord blood of newborns contain special sub-types of progenitor cells, which are able to differentiate into mature endothelial cells. Under conditions of tissue regeneration or hypoxia they contribute to re-endothelialization of denuded vessels and neo-angiogenesis
In the present study we sought to determine whether adult mouse lungs can be used as donor organs for the isolation of vascular endothelial cells, to be used
During a literature search for similar cells, we found a very recent report by Alvarez and colleagues, who describe that rat lung microvessels are enriched with endothelial progenitor cells (EPCs)
Mouse lung EPCs possess hemangiogenic and lymphangiogenic potential
Mouse lung EPCs as described in our study are vasculogenic in Matrigel assays
Together with the recent report from Alvarez et al. our findings supports the premise that the pulmonary microvasculature is a rich endothelial progenitor niche, which may regenerate blood and lymphatic endothelium to maintain vascular homeostasis and contribute to tissue remodelling and repair
A further interesting aspect, which however, could not be resolved in this study, is the exact localisation of the EPC niche within the lung. In addition to a putative direct position in the endothelial monolayer, a localisation in the vessel wall or in perivascular tissue may be possible. Moreover, reliable markers to prospectively identify EPCs in the intact lung are still lacking. The existence of human EPCs and stem cells that are capable of differentiation into mature endothelial cells, hematopoietic and local immune cells has been studied in fragments of the internal thoracic artery of adults
Angiogenic processes within the postnatal pulmonary circulation are difficult to study and the phenomenon is not generally accepted. Angiogenesis may occur within the lung systemic circulation to repair pulmonary circulation defects and to restore blood flow
With our results presented here, we finally demonstrate, that mouse lung EPCs are intrinsically capable of forming blood and lymph vessels
Immortalized Endothelial Cells and Tumor Cells
The generation of endothelioma cell lines (polyoma middle T-immortalized mouse endothelial cells) from mouse embryos (Balb/c and C57Bl/6) has been described previously
All studies involving the use of mice were approved by our local institutional animal care committee. The protocols were also approved by the regional council on animal care and correspond to the requirements of the American Physiology Society.
Isolation and culture of mouse lung microvascular endothelial cells
Mouse lung microvascular endothelial cells (MLMVECs) were isolated from mouse lungs using a magnetic cell separation method and cultured as previously described
Cell growth
MLMVEC cells were seeded at 1 × 104 cells/cm2 in gelatinised separate six-well plates or later in small cell culture flasks (25 cm2). The medium contained DMEM and 15-20% FCS (MBE medium) and has been described previously
Proliferation assays
MLMVECs and endothelioma cells were seeded at 1.5 × 104/well in separate 48 well plates (1,1 cm2/well) in MBE medium. Following 72 h incubation at 37°C with 5% CO2, the medium was replaced and this time point was designated day 3. Proliferation studies were performed in duplicate. Cell growth was determined after day 3 at 24 h intervals, using an electronic cell counter (Casy Systems, Germany). Results denote the average of duplicate measurements repeated at least once. For the proliferation assay in the presence of growth factors, cells were seeded in minimal medium (in 5% FCS w/o ECGF) supplemented with the growth factors and measured over 4-6 days with one growth factor exchange after three days. For proliferation assays in the presence of VEGF-A (mouse VEGF164) we used 5 and 20 ng/ml growth factor.
Limiting dilution analysis
Limiting dilution analysis was performed as described earlier with some modifications
Immunophenotyping by FACS Analysis
MLMVECs and other mouse endothelial cells were treated with accutase (Cat.no. L11-007, PAA, Austria) and suspended at 1 × 105 cells per tube in 1 ml microtubes containing 200 μl of cold PBS (calcium and magnesium free) supplemented with 2% FCS. For the immunostaining, cells were incubated for 15 min on ice. All cells were rinsed with cold PBS/2%FCS and incubated with the primary antibody or with fluorescein-conjugated lectin or isotype control antibody for 30 min on ice in the dark. Cells were rinsed in cold PBS/2% FCS and then incubated with the corresponding fluorescent dye-conjugated secondary antibody. At the end of antibody incubation, cells were transferred to flow cytometric tubes (Micronic, The Netherlands) and subjected to flow analysis using FACS Calibur (Becton Dickinson). We used the following primary antibodies: CD31 (rat clone MEC13.3; BD), CD34 (rat clone RAM34; BD), CD45-FITC (rat clone 104, BD Pharmingen), CD102 (rat clone 3C4(mlC2/4); BD), CD105 (rat #6J9; Reliatech), CD144 (rat clone 11D4.1; BD), VEGFR-2 (rat clone Avas12α1; BD), VEGFR-3 (rat Cat. no. 103-M36, Reliatech), Lyve1 (rabbit Cat. no. 103-PA50AG; Reliatech), podoplanin (hamster Cat. no. 103-M40; Reliatech), PV1/Meca32 (rat; U. Deutsch), LA102 (rat Cat.no. 103-M152; Reliatech) and LA5 (rat, Cat.no. 103-M154; Reliatech). The following primary antibodies for HSC were used: CD117-PE (rat 120-002-406; Miltenyi Biotech), CD133 (rat clone 13A4; eBioscience, U.S. and rat clone MB9-3Ga, Miltenyi Biotech, Germany) and CD41-PE (rat clone MWReg30; eBioscience, US). For lectin binding we used the FITC-conjugated
Measurement of NO production
The cell-permeable diacetate derivate, DAF-2 DA (4,5-Diaminofluorescein.diacetate) was used to measure NO production (Cat. no. ALX-620-056; Alexis, Switzerland). After loading and subsequent hydrolysis by cytosolic esterases, DAF-2 is released, which is relatively non-fluorescent at physiological pH. In the presence of NO and O2, DAF-2 is converted to the fluorescent triazole derivated, DAF-2T
To study sprouting angiogenesis
Green fluorescent protein and luciferase transfection
Lentiviral constructs encoding green fluorescent protein (GFP; pPREGFPSIN) and luciferase (LUC; pJSCMVLuc) without antibiotica resistance, were produced as previously described
MLMVECs were trypsinized, counted and cultured overnight in hanging drops to allow predifferentiation of cells in spheroids (~ 10,000 cells/spheroid). Matrigel was supplemented with 500 ng/ml bFGF and VEGF-A and used with or without spheroids. For each injection, about 30-50 spheroids were mixed in 300-400 μl unpolymerized Matrigel at 4°C. The mixed solution was injected subcutaneously (left and right dorsal region, two plugs per animal) into mildly sedated (ketamine 100 mg/kg ip) Balb/c female mice using a 20-gauge needle. The injected Matrigel polymerizes at body temperature and becomes a plug. As controls, Matrigel was injected on the left side without cells but with growth factors. 10-12 days after injection, Matrigel plugs were excised and treated overnight with methanol/10% DMSO. Plugs were further immersion-fixed in 4% paraformaldehyde, treated with saccharose and embedded in frozen section medium (Neg50; Richard-Allan Scientific, U.S.).
Immunodetection of MLMCECs
Excised Matrigel plugs embedded in frozen section medium were used to prepare cryosections of 16-20 μm thickness. Non-specific binding of antibodies was blocked by incubation with 1% bovine serum albumin (BSA) for 1 h before incubation with primary antibodies. The following primary antibodies were used for single and double-staining: Anti-mouse CD31 (rat clone MEC13.3; BD), anti-mouse CD45 (rat clone 104; BD Pharmingen) anti-mouse podoplanin (hamster Cat. no. 103-M40; Reliatech), anti-mouse CD105 (rat #6J9; Reliatech), anti-GFP (rabbit Cat. no. ab6556-25, Abcam, U.K.) and anti-mouse LA102 (rat Cat. no 103-M152).
The sections were incubated with primary antibodies for 1 h. After rinsing, secondary antibodies were applied at 1:100 or 1:200 dilution for 1 h. We used goat anti-rat TRITC-conjugated, (Jackson IR, U.S.), donkey anti-rabbit Alexa488 (Pierce, Rockford, U.S.), goat anti-hamster Alexa594, goat anti-rabbit Alexa594 and goat anti-rat Alexa350-labeled (MoBiTec, Göttingen, Germany). DAPI reagent (Hoechst Dye) was added to stain nuclei.
After being rinsed, the sections were mounted under coverslips with Fluoromount-G (Southern Biotechnology, U.S.). They were studied with a DM500 B epifluorescent microscope and Axioplan 2 LSM 510 laser-scanning microscope (Zeiss, Germany).
Anchorage-independent growth on soft agar
MLMVECs and mouse tumour cells (βTC) as positive control were suspended in MBE medium and seeded at a density of ~1 × 103 cells/cm2 in 48 well plates coated with 0.8% soft agar in MBE medium. Cells were incubated at 37°C and 5% CO2. After 8-10 days, plates were studied with phase-contrast microscopy. Efficiency of anchorage-independent growth was determined by the capacity of cells to form colonies (> 100 cells) within the agar. Experiments were performed as duplicates and repeated at least once.
BEC: blood endothelial cell; LEC: lymph endothelial cell; FACS: fluorescence activated cell sorting; EPC: endothelial progenitor cell; MLMVEC: mouse lung microvascular endothelial cell; VEGFR-2/-3: vascular endothelial growth factor receptor-2/-3; ECGF: endothelial cell growth supplement from bovine brain; HGF: hepatocyte growth factor; VEGF-A: vascular endothelial growth factor-A; bFGF: basic fibroblast growth factor; TNF-α: tumor necrosis factor alpha; GFP: green fluorescence protein; PmT: polyoma middle T oncogene.
Authors' contributions
JS contributed to the studies involving isolation of EPCs, flow cytometry studies and immunofluorscence. MR contributed to in vivo studies and immunofluorscence. KB made substantial contributions to immunofluoescence studies and analysis of data. GR contributed to in vitro differentiation studies and acquisition of data. AMS and SN performed the research, and contributed to the acquisition and interpretation of data. MB contributed to the animal studies and interpretation of data. BB provided specific research material and was involved in interpretation of data. TM contributed to the studies involving lentiviral transduction of primary mouse cells. JW and HAW contribute to the overall conception and design of the experiments, in drafting the manuscript and give final approval of the version to be published. All authors have read and approved the final manuscript.
The studies were supported by the Deutsche Forschungsgemeinschaft and the Gesellschaft Deutschsprachiger Lymphologen. We would like to thank Maria Höxter and Susanne zur Lage for their valuable contribution to cell sorting and FACS analyses, and Dr. Peter P. Müller for his support in the mouse-
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Originally released in 1985 on the Black Victory label this rare album is to see the light of day again courtesy of Dug Out.
It's a one riddim album that plays on King Tubby's classic Tempo riddim and is hailed by many as being one of the best of its kind.
There are only eight short songs, but each one is sublime in its own way. The first four cuts are vocal versions from such respected artists as Willie Williams, Chris Wayne, Ras Menlik Dacosta and Sugar Minott, who also co-produced the album with Bullwackie and each of these artists brings their own distinct style to the table.
The final four are instrumental cuts with the smooth sax of Jerry Johnson, who more recently appeared on Burning Spear's 2009 Grammy award winning 'Jah Is Real' drifting on top of one while the other three are from The Black Roots Players who add real dread and menace with their 'Slow Tempo' version.
This album with its meticulous and mesmerising mix of electronic and analogue techniques is sure to appeal to fans and collectors of the digital revolution that took place within 1980's reggae.
Mr Money Man Riddim
By Angus Taylor
New Albums from Etana and Protoje
By Erik Magni
Paint Jamaica
By Erik Magni
Live My Life Again, Perfect Giddimani final album
By Erik Magni
Sweet You, Sour You by Freddie McKay
By Erik Magni
Jah Sun - Between the Lines
By Erik Magni
Wise & Wild by Patois Brothers
By Erik Magni
Presenting Kingston All-Stars
By Erik Magni
Fought For Dis by Sizzla
By Erik Magni
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FRIDAY 'ROUND-THE-HORN. - *Jim Fourniadis -- genius behind Rats of Unusual Size,**and producer of the first Reverb Motherfuckers album**(how's *that* for HOF credentials?) -- **did ...
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Senco Products PC1130 1 1/2 Hp 2 1/2 Gal Compressor
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- Cast iron cylinder for superior pump life
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