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Noll 50k/100k Stacked EQ/EQ Control
This potentiometer design is for various space-saving applications, depending on the resistance value and taper. Such as volume/balance or active controls.
50KB /100KB+100KB Stacked Control for active use
Threaded section is M10 x 0.75, Length 10mm, Solid Shaft 4mm upper, 6mm lower.
Cavity depth of 27mm required.

subset: commoncrawl


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Sheriff's Officer Jim Terry, a military veteran, was riding his motorcycle to work Sunday when he was hit by a drunk driver who then fled the scene.
Illegal alien Oscar Salmeron, 42, had already hit another vehicle and failed to stop. As he was fleeing that accident, he then hit Officer Terry on his motorcycle, and again sped away.
Denton Police were able to catch and apprehend him, and he is now being held in the Denton County Jail on a $35,000 bond and multiple charges, including intoxication assault with a vehicle causing serious bodily injury. He also has a hold by the U.S. Immigration and Customs Enforcement.
Officer Terry is in critical condition in a local hospital, with multiple broken bones and extensive injuries. He has undergone one surgery and is expected to have more surgeries Wednesday and Thursday.
Terry is a veteran, and has been with the sheriff's department for two years. A GoFundMe account was created for his medical expenses.
To see more stories like this, sign up below for Dennis Michael Lynch's email newsletter.
Sign up to get breaking news alerts from Dennis Michael Lynch.
Debate Arises in Vermont Town over Acceptance of 100 Syrian Refugees


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Cooke Barracks Guestbook
All fields marked with a * are required.
The CookeBarracks.com Guestbook is a "roster" for others to "Google" others. It's geared to be a single-entry, one-time post that includes your name and an email link so others can find you.
On the other hand, the CookeBarracks.com Forum is geared for public, on-going, discussion amongst anyone who cares to participate. It can be reached at: CookeBarracks.com Forum.
There's also a CookeBarracks.com Photo Album that can be reached at: CookeBarracks.com Photo Album.


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Call our experts: 888.808.FINE
Shop Personalized StationeryCarlson Craft Wedding Invitations Customize any Address Stamp to match your style!
Select a quantity and printing method. Changes are easily made later.
Gilded gates that lead to marble foyers that lead to splendid celebrations, all of which began with a classic pearl white invitation graced with a swirled frame that prints in the same ink color as your personalized text. Shown with optional motif, MOT1308A.


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Children need to stay occupied to keep them from eating dirt because it looks like chocolate cake or eating chocolate cake, which they are also not allowed to have right now. Keep their hands busy with this Groupon.
Choose from Three Options
- $5 for single-day fair admission for two children aged 3–13 (up to a $10 value)
- $7 for single-day fair admission for one adult and one child (up to a $15 value)
- $10 for single-day fair admission for two adults (up to a $20 value)

Inside Navy Pier's Festival Hall A, the two-day Chicago Toy & Game Fair runs from 10 a.m. to 6 p.m. on Saturday, November 17, and from 10 a.m. to 5 p.m. on Sunday, November 18, presenting attendees with toys and games from around the world. This year's events include a yo-yoing contest, performances from Blue's Clues Live, and a national Settlers of Catan qualifying competition. More than 100 companies will be previewing new games, including Bananagrams, Goliath Games, and PlaSmart. Children's favorite Elmo, from Sesame Street Live: Elmo Makes Music, will visit the fair from 10 a.m.—11 a.m. on Saturday, November 17. Educators and librarians, scouts in uniform, and active or retired service members get in free.

Though this merchant sometimes offers a discounted price online, this Groupon is still the best deal available.
Chicago Toy & Game Fair
When toy and game inventor Mary Couzin attended Essen Fair in Germany in 2001, she was moved to see families playing together and the excitement European crowds greeted toy and game designers. Two years later, she'd founded the Chicago Toy & Game Fair at Navy Pier. Chicago is Toy & Game Inventing Capital of the World, where many beloved playthings such as Beanie Babies, Rock 'Em Sock 'Em Robots and TMX Elmo were created. Couzin continues to throw an annual two-day extravaganza the weekend before Thanksgiving. Along with international guests, inventors of classics such as Bop It, Operation, Twister, Nerf and Jenga gather to sign copies of their famous creations. Giant toys and games, interactive exhibits, STEM activities, tournaments, costumed characters, stage events, Young Inventor and Young Tech Challenges and costumed Star Wars fans gather for lunch.


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Grab these new Charmin coupons that popped up this morning. We have a stock up price at Walgreens this week where Charmin products are included in a Balance Reward Points promotion. You can get them for only $.30 per double roll!
$1/1 Charmin Ultra Strong or Ultra Soft 12 ct or larger printable coupon (preclipped)
$.50/1 Charmin Basic printable coupon (preclipped)
At Walgreens:
BUY THREE CHARMIN ULTRA SOFT OR ULTRA STRONG 16 ct – on sale $7.99
Get 5000 Balance Reward Points when you buy $20 worth of Tide, Dawn or Charmin Products (value $5-$6.25)
use three $1/1 Charmin Ultra Strong or Ultra Soft 12 ct or larger printable coupon (preclipped)
Price at register: $20.97 but receive 5000 BRP
Net Price: as low as $14.72 ($4.91 each or $.30 per double roll!)
Never miss another hot coupon, deal or freebie again! "Like" Couponaholic .NET on Facebook, Follow us on Pinterest, Follow us on Twitter, Follow us on Instagram or Subscribe to our Daily Email Newsletter!


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From Our Editors
Before settling down in Arizona, Tracy McDonald made her home all over the world. She spent time in Senegal while volunteering for the Peace Corps and lived in Madagascar while participating in a coral-reef sustainability and diving expedition. She also studied yoga in India and went on to become a yoga instructor, as well as a board-certified polarity instructor, a cranial-sacral therapist, and an expert in issues related to weight and body image. She now uses her expertise to help clients revamp their lives inside and out with such services as Ashtanga yoga sessions, cranial-sacral sessions, and lifestyle coaching.


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Now that this great 2013 is coming to an end, everyone is wondering what will follow in 2014. There is a formula that can help us frame our outlook. It goes like this:
Return = Dividend Yield + (1 + Earnings Growth) X (1 + P/E expansion/contraction) - 1
The following table uses this formula to peek into 2014. The cell highlighted in yellow - 6% earnings growth and an ending P/E of 15 - is the average long-term situation. In other words, if 2014 is "average" we'll see a 16% loss. But what if it's not average? The purple cells highlight a band around the average and indicate a performance range between a 13% gain and an 18% loss.
Return Forecast for 2014 (1 Year)
Source: PPCA Inc
We can also use the same formula to look beyond 2014, to the end of the decade, as shown in the following table:
Return Forecast for 2014-2019 (6 Years)
What do you think 2014 and beyond will bring?
Disclosure: I have no positions in any stocks mentioned, and no plans to initiate any positions within the next 72 hours. I wrote this article myself, and it expresses my own opinions. I am not receiving compensation for it. I have no business relationship with any company whose stock is mentioned in this article.


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The original picture and the last shot would prolly be the way the car was shipped from Hethel. Race cars are always evolving. The picture with Parnelli exercising the beast was obviously taken after the car had been raced. They prolly figured somewhere along the line that the car needed a little more downforce than the wedge shaped body could give them. This was right around the time that we all discovered wings....Originally Posted by GoodStone1969
"Doc, just set them fingers sose I can hold the wheel"
James Hurtubise, June, 1964
The first picture from Indy2 shows Mario in what I recall as the McNamara chassis indy car. In his book, Mario was not very happy with it. In fact he hated it...
Here is Gordo in his Stearman/cosworth at Milwaukee:
One of the Buick engines, from Sneva's car, 1989
Kenny Brack, Indy 1997
Scott Harrington, Indy 1997
Firestone (the real owner of the turbines) decided that the cars should run the entire circuit and as the year went on Parnelli (their main guy at the time) ended up running the turbines with STP remaining as sponsor. As the importance of downforce started to be understood the Parnelli group started adding their own touches to the cars including new intake scoops, a ducktail kickup tail and the two wings/spoilers on the front. These worked pretty well on the shorter ovals and the road courses but the cars always had trouble with braking. At the end of the season Firestone was done and Parnelli became the owner of the cars and still owns the #60 turbine to this day.Originally Posted by GoodStone1969
Yeah, but you would a basket full of $6 ball bearings...Originally Posted by ZOOOM
And the colors on the numbers are flipped and there is a spoiler on the tail.Originally Posted by GoodStone1969
Quite true, Mario hated the 1970 car, and had an incident where he drove through the grass in turn two (IIRC) mid-race, and said it was much better afterwards.what I recall as the McNamara chassis indy car. In his book, Mario was not very happy with it. In fact he hated it...
As much as Mario hated the 1970 car, Big Andy stuck with Francis McNamara as his car builder for 1971, and the 1971 car was even more bizzare, and Mario hated it even wose.
Originally Posted by Kevracer
In one of those Goodyear films about the race, the narrator said that running through the infield had caused the McNamara's suspension to "unlock". No other details or explanation. Never heard any other explanation, agreement or disagreement. FWIW
Anybody know what track these pics are from? Milwaukee?
In "Driving Passion" with Gordon Kirby, Mario said of the '70 Indy race, "The car was OK for the first few laps. Then I was an accident waiting to happen. I thought about comming in about ten times because I was dangerous out there.
(avoiding an accident, he had to drive through the infield in turn two) I heard something snap and I thought I had broken something.Then the race went green and it was a whole new racecar"
Apparently a spring had loosened up when the car was up on the jacks for a pitstop. The spring didn't reseat itself on the spring perch and the car reacted like Mario had jacked about 250 pounds of crossweight into the car. "So actually I had been running most of the race, to that point, on three wheels!"
No wonder he didn't like it...
Kevin Cogan 1986 Mid Ohio
Jacques Villeneuve Indy 1994
Scott Goodyear during 1997 Indy 500
Great pictures!
Gurney, at the time, said he couldn't believe thirty three of the best drivers in the world, couldn't drive down a country road without crashing into each other....
The methinol that Salt sprayed into the crowd caused the track to remove the folding chairs just outside the front straight wall...
Keep the pictures comming!
"Anybody know what track these pics are from? Milwaukee?"
No,it was Langhorne PA
It was a lot more serious than the glib blurb that Sam escaped with minor burns. He was trapped in the car for quite awhile with the windscreen,etc melting all over him. He was extremely lucky not to have burned to death in there.
BTW I`m loving the photos in this thread.
Esp those of Sneva33.
Did you ever get any photos of my uncle Jerry Karl????
As promised awhile back, I finally found them. Not the best shots ever but you get the idea. Chip Mead, Indy 1981. Better late than never?
Thanks ovalmeister,
I looked but if I ever had any shots or slides of that car I could not find any.
Jeff McPherson (I think) Mid Ohio 1986
1994 Indy, Nigel Mansell's car w/Bryan Herta driving
Al Unser Jr. Road America 1995


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The Sub Zero Competition Mod
The Sub Zero Competition Mod
The Sub Zero is a purpose built competition mechanical mod. From the ground up, every feature was tuned for complete reliability and maximum conductivity.
The Products on this website are intended for Adults Only! By entering this website, you certify that you are of legal smoking age in the state in which you reside.or Exit


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Went with some friends to Church and State last night and had an excellent meal. I have not been a huge fan of Arroyo's places, so I wasn't sure what to expect. But the space itself is lovely – albeit loud – and the food and service top notch. Great drinks at the bar, and the bartender went out of his way to make sure we were well taken care of. All the staff, in fact, from waiter to hostess to bus staff, really takes care of you.
As for the food: we ate a lot! We started off with Escargot… perhaps the highlight of the night. Even if you think you don't like snails, and I don't particularly, this is one of the best dishes I've had in a long time. You get 5-6 individual dishes topped with puff pastry and filled with garlicky goodness. We also tried the Bone Marrow flan. I was not a huge fan, mostly because I am not a fan of the consistency of flan. Everyone else loved it, and it came with a terrific radish and parsley salad. We also had one of the tarts. Also terrific. They are huge and served on a very cool wooden board. My only suggestion here would be for a half size for an appetizer. But I am not complaining.
We tried all three salads: frisee, egg and lardon salad; baby lettuces with herbs and a citrus vinaigrette; endive. All were excellent. The dressings in particular were top notch. If I had to choose one, go for the endive.
Then as entress we had: steak frites, cassoulet, mussels and mac and cheese. The steak frites was served sliced, not always my favorite, but the meat was excellent, perfect temperature with a terrific char, and the fries outstanding. I loved my Cassoulet. It was vegetarian and different than a normal cassoulet… I think the type varies night by night. But when something is this good, who cares if it is not the norm. The vegetables were fresh and perfectly cooked, and it had a creamy, flavorful sauce. The mac and cheese was good. My friend who ordered the mussels liked not loved them. She felt they could have had more flavor. But it would be tough for anything to beat the juice that came with the escargots.
All said… great service, great food. I look forward to returning to hit some of these again and also see what the roast chicken is like!


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Bremerton Central Lions 28th Annual Dinner & Auction
Saturday, November 7, 2015
Doors 5:30 p.m. | Dinner 7 p.m.
Tickets $75 (RSVP By Friday, October 30)
The Bremerton Central Lions annual fundraiser with all proceeds benefiting projects in the local community.
About The Bremerton Central Lions:
For 90 years, the Bremerton Central Lions Club has been an integral and unwavering partner with the City of Bremerton and its people. The Club and its Charitable Foundation have donated hundreds of thousands of dollars to our schools, parks, entertainment venues, and to countless non-profit organizations that serve those of us in need.
In addition, Lions can be seen volunteering throughout the community. Feeding the hungry, assisting in vision screening, erecting fences and doing cleanup around shelters are just a few examples.
During the past year - as usual - Lions changed the Admiral Theatre marquee over 100 times; and tonight in the Fund-A-Need portion of our Auction, we seek to assist the theatre in its effort to replace their manual marquee with a digital one, to "Keep Bremerton Glowing." Don't worry, after that's done those Lions will find something more to do. After all, the Lions motto is, "We Serve!"
Sponsored By: Admiral Theatre, Holland America Line, Kitsap Credit Union, Achieve Eye & Laser Specialists, Kitsap Bank, Kitsap Sun


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From the opening monologue of the film, it's immediately apparent that we're in for a different Iron Man. Tony Stark narrates the opening scenes and his words make it clear that the pompous playboy we loved in Iron Man, but grew tired of in Iron Man 2, is now a mere shadow of his former self.
He tells us of a night in Switzerland, 13 years prior to the events of Iron Man 3, where we meet Maya Hansen (Rebecca Hall) and Aldrich Killian (Guy Pearce). Hansen is a beautiful and brilliant, young scientist on the verge on opening up the next phase of human evolution. Killian is another brilliant scientist, clamouring for the attention of Stark but is promptly ignored due to his lack of breasts. The events of this night create the most despicable villain Iron Man has seen yet.
Fast forward to present day and we meet The Mandarin (Ben Kingsley). A modern, terrorist figure whose propaganda videos interrupt the airwaves of American television while his bombs are killing the American public. And when Stark's friend Happy (Jon Favreau) is caught up in one of The Mandarin's explosions, the plot quickly turns to revenge.
It is a plot which succeeds. It succeeds in maintaining a wealth of depth, there is so much going on at all times, while not succumbing to the shortcomings of other films which try to juggle so much (see Spider-man 3). It takes twists and turns which genuinely surprised me and kept me on the edge of my seat for it's long, 130 minute run time.
Ben Kingsley shines as The Mandarin. His performances in the aforementioned propaganda videos are stunning, his narration of the heinous acts of terror committed is cool and disturbing. Kingsley commands your full attention whenever he is on screen. He even shines in later scenes when the character takes an unexpected and, potentially, controversial turn.
Robert Downey Jr is fantastic as a more vulnerable Tony Stark. The relentless arrogance which plagued the character in Iron Man 2 has been mellowed for a man who is suffering some post-traumatic stress from the events of The Avengers. "Nothing has been the same since New York," he tells us. The one-liners and smug quips remain, and Downey Jr is as brilliant as ever at delivering them, but it is in the moments of weakness that he truly shines. It is worth mentioning a section in the middle of the film in which Stark gains a young sidekick (played by Ty Simpkins) as the banter between the two leads to some of the film's best comedy.
Aldrich Killian is the best villain we've seen in an Iron Man film and Guy Pierce plays him to perfection. He gives us a slimy and deplorable character who we hate for all the right reasons (as opposed to Iron Man 2's Justin Hammer who was just annoying). The contrast between the geeky scientist, who Stark blew off in the opening scenes, to the man he becomes is staggering.
The women of the film are completely over-shadowed. Rebecca Hall's Maya Hansen is never fully fleshed out and Pepper Potts (Gwyneth Paltrow) remains a damsel in distress.
Now, it wouldn't be a Marvel film without the obligatory Stan Lee cameo appearance. This is one of the blink-and-you'll-miss-it kind.
No matter how the good the plot, or wonderful the performances, the reason that most of us love Iron Man is the spectacular action scenes and, on this front, Iron Man 3 succeeds with flying... everything. There are explosions galore, some astounding special effects and, without ruining anything, the scene at Stark's mansion is breathtaking. These scenes are over the top, and ridiculously far-fetched, but they are also inventive and a complete thrill ride. Iron Man 3 delivers the best action of any Marvel film thus far, The Avengers included.
The original Iron Man was fun movie because it introduced us to this mischievous and lovable rogue called Tony Stark while presenting the audience with some intense action sequences. Iron Man 2 upped the ante as far as action was concerned but the character became too arrogant to bare. Neither film gave us a villain to truly hate, one who we clamoured to see get their comeuppance. Iron Man 3 gives us a protagonist to love again, the best action we've seen and the villain that the series has lacked so far. It's the complete Iron Man package and a tremendous summer blockbuster.
Follow me on Twitter @kevinodonnell91


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Our Services
Fox Valley Medical & Dental Centre
The Fox Valley Medical & Dental Centre operates in close association with Sydney Adventist Hospital. The Centre is accredited with AGPAL (Australian General Practice Accreditation Limited) and is firmly committed to providing the very best care for you and your family.
Male and Female General Practitioners are available, offering an extensive range of family healthcare services including:
- General Consults
- Travel Vaccinations and immunisation including Yellow fever (by appointment)
- Immunisation of children
- Pap Smears & Breast Examinations
- Insurance, Drivers Licence & Lifestyle evaluation checks by appointment
- Executive Health Checks – a 2-hour complete Health Assessment
- Health Care Assessments for patients over 75 years
- Electrocardiograms (resting and exercise)
- Minor Operation Theatre
- Pathology collection
Other services also available at the Fox Valley Medical & Dental Centre include:
- Breast Screening (free for women aged 50-74). This service is run in association with BreastScreen NSW. Phone 13 20 50 for an appointment at the Fox Valley Medical & Dental Centre's Unit.
- Outpatient Diabetes Education
- Clinical Psychologist
- Dental Appointments
Fox Valley Medical & Dental Centre is located next to the Sydney Adventist Hospital at 187 Fox Valley Road, Wahroonga.
Patient parking is readily available adjacent to the centre at a minimal fee. Disabled parking is also available. Patients should enter through the boom gates.
Click here for a printable site map and directions.
Practice Fees
Fees are set within those approved by the Australian Medical Association. Payment of account on the day of consultation is appreciated. Pensioners and Health Care Card holders will be bulk billed on presentation of their cards. There is a surcharge for weekend appointments.
Evening, weekend or public holiday home visits will be attended to by Doctors from the After Hours (Family Care Medical Services). They can be contacted by phoning 02 9410 3944. Details about membership fees and forms are available from the Fox Valley Medical & Dental Centre.
Further Information and Appointments
Consultations at Fox Valley Medical & Dental Centre are by appointment. For further information, and appointments for all GP services and Executive Health Checks, phone 02 9487 9700.
For Dental appointments, please phone 02 9487 9713.
The Fox Valley Medical & Dental Centre is open:
|Monday – Thursday
|8.00am – 6.30pm
|8.00am – 4.00pm
|8.30am – 12.15pm


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Real World article
(written from a Production point of view)
(written from a Production point of view)
Kirby was a female Human mentioned in a script [1] for Star Trek: First Contact.
In the script, her dead body is found by a security guard called Lange in Zefram Cochrane's mission control room, shortly after the Borg attack. In the same room lie the corpses of Rippert, Mitchell, and DePaul.


text: Huawei retains largest share of radio access network market in Q3 2013 03 Dec 2013 03:10 PM Alcatel-Lucent trades places with NSN to take third place and Samsung makes largest sequential gain, according to ABI ResearchHuawei maintained its No.1 rank in the radio access network (RAN) market with a share of 28.1% for the third quarter of 2013, according to ABI Research. Its share of the market was down 3 points from 2Q 2013 and up 3.8 points from the year-ago quarter.Alcatel-Lucent increased its RAN revenue by 20.1% sequentially to gain the No.3 spot ahead of NSN, which dropped to No.4 with RAN revenues slightly ahead of normal seasonality for the market. Ericsson maintained its No.2 rank position with RAN revenues in line with normal seasonality.ABI Research estimates Samsung posted the largest sequential growth in RAN revenues this quarter at 44.6%, which is also 101.4% ahead of the year-ago quarter."Alcatel-Lucent's results were due to strong growth in LTE driven by the US market in addition to positive trends in both the APAC and EMEA regions with LTE revenues more than doubling year-over-year, thanks to the company's LTE overlay strategy," said principal analyst Nick Marshall.According to NSN its year-on-year decrease in mobile broadband revenue was primarily due to lower sales in WCDMA, GSM, and CDMA. LTE revenue was flat this quarter as higher sales in North America, Europe and Latin America offset lower sales in Japan and Korea."Samsung's No.5 ranked position is unchanged from the previous quarter but the company also attributes its large sequential growth in RAN revenue to increased LTE sales in overseas markets," continued Marshall.ABI Research has estimated the market share for the top 5 vendors and this is shown in the table below.Top 5 RAN vendor Q3 2013 market share1 Huawei - 28.1% (Q2: 31.1%)2 Ericsson - 21.8% % (Q2: 23.4%)3 Alcatel-Lucent - 16.9% % (Q2: 14.3%)4 NSN - 14.9% % (Q2: 15.9%)5 Samsung - 9.5% % (Q2: 6.7%)These findings are part of ABI Research's Macro Base Stations Research Service.James Atkinson EditorLinkedIn |Google + |Email Leave a CommentPlease write code to prove you're humanMore on... HuaweiHuawei, Vodafone and Qualcomm create LAA ready networkMWC17: Cloud and video are the way forward for operators says Huawei


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Paul Davis <paul.joseph.da...@gmail.com>
|Re: code sharing across databases
|Sat, 04 Jun 2011 19:12:26 GMT
On Sat, Jun 4, 2011 at 3:08 PM, MK <email@example.com> wrote: > I have a few databases with _design pages that include modules > which get used within views/shows etc via require, eg: > > doc.password = require("lib/sha1").sha1(req.form.pass); > > These are all generic. Right now, they are part of the _design, > meaning they are replicated for each database, which is a bit of a > hassle if they are changed (I have to update all the db's they are used > in). Is there a way I can require(code) within a _design from an > independent database of common functions, so that I can just make > changes to that and not have to recreate the _design for every db? > > I am not using couchapp (and won't be) altho I did steal the /lib idea > from there. > > -- > "Enthusiasm is not the enemy of the intellect." (said of Irving Howe) > "The angel of history[...]is turned toward the past." (Walter Benjamin) > > Nope. And likely never will be. It'd be kinda weird to have to deal with some server wide replication of that information.
|View raw message


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Yoshiko Takahashi is Takahashi's wife and the mother of Kazutoshi Takahashi.
|STUB Alert!
|What's going on here? This article is a stub!
|This article or section is a stub and can be improved in areas such as grammar, style, wiki-formatting, spelling and expanding.
Help Beelzebub Wiki by editing this article or section !


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Auldfolkie - Music For Sale
Musical Merchandise For Sale: LPs, 45s, CDs, 8-Tracks
VHS Video Tapes For Sale - coming soon
Books For Sale - coming soon
Other Bothy Press Enterprises - coming soon
Email me with your Order or Want List. I will get back to you shortly with availability and total price. I accept online payments using PayPal. I also accept checks or money orders for US orders.


text:
During this encouraging commencement speech, Ellen DeGeneres addresses the graduation glass of Tulane University. She moves the audience when she talks about her difficult journey through life and evokes laughs as she explains how her definition of success has changed over the years.
Ellen talks about many emotionally heavy topics during this encouraging commencement speech. She addressed the graduating class by saying that -- in her opinion -- the most important thing in your life is to live with integrity, to live as an honest and passionate person and to contribute in some way. Above all else some of the most desisting things that have happened to you will teach you the most.
Ellen ends her encouraging commencement speech by offering one last piece of viable advice before she dances off the stage. She reminds the young people who are breaking into the job market to show their brains and be true to themselves.
The Solution for Gen Y
Life After Graduation
The Mythical Value of Dreaming
Potential of Young Generations
A Life of Action
Ellen Degeneres Keynotes
Speeches by Ellen DeGeneres reflect the talk show host's remarkable sense of comedic timing and...
Need Inspiration?
Get inspired by 3,000+ keynote speaker videos, courses and our founder, the top keynote speaker on innovation.
Ellen DeGeneres Gives A Personal, Encouraging Commencement Speech
- By: Jessica MarrenMay 24, 2013
References: youtube & flavorwire
References: youtube & flavorwire


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Hey MQL4ers
Having trouble with my trailing stop function.
Can someone please clarify ALL situations that lead to this error?
Aware of traditional Stop too close to market which is definitely not issue and stop in wrong format, which should also not be an issue... I don't think I need help with code at this stage but will post if need be... I'd rather this topic to be definitive guide to 130 errors (as opposed to ANOTHER code specific fix!).


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People, people, people...I get them. I will probably get you. If it takes me more than an hour to get you, I will be intrigued..
Im a thinking, caring person.
settings...lighting, colors, smells...fall.
simple things like picking up my laundry and smelling my clean clothes.
I am currently very excited the rest of my life
could not ask for better friends or family. I am ridiculously fortunate to have such amazing people in my life.
I would do anything for them.
I am to complex, to answer, and in three words
Sharing my views, but also at listening to yours..
I am very good at finding the "Dives" places that quickly become staples in my life- like places that others may feel are " shady" but to me, they have character
Making sure that everyone around me is comfortable!
Witty banter and sarcasm.
Another thing people notice is my hair. I shave my head, not because I have to (I have pictures to prove it) but because I like the look of it.
Driving Miss Daisy
Mob wives
Adult Alternative
R & B soul
steak / salad
As for movies I love the classics... Pretty Woman, Driving Miss Daisy and Donny Darko.
If it were all to end at this very minute would I be satisfied?
The most strategic and appealing placement of every picture on my wall.
Where I will be physically, spiritually, emotionally, and psychologically five minutes, five hours, five days, five months, and five years from now....
I have a keen sense of smell.


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shows at bristol old vic...
Blahblahblah - July
Vanessa Kisuule: SEXY
Presented by Bristol Old Vic, Tangent Books and Anna Freeman
Part of Bristol Old Vic's Studio Walkabout Season
'Sexy' is a word a lot of us are uncomfortable applying to ourselves. Vanessa Kisuule certainly doesn't feel she fits the bill. She really wants to, though. Through comedy, spoken word, dance and various states of undress, Vanessa fumbles her way through the infinite contradictions in her head to find (hopefully) the definition of what sexy really means.
Vanessa Kisule: Sexy
Part of Blahblahblah
31 July
The Wardrobe Theatre
Ages 14+
@BristolOldVic | #Blahblahblah
More from Blahblahblah
In summer 2016 we began redevelopment works to transform our King Street entrance into a welcoming, vibrant space and build a brand new Studio theatre designed by award-winning architects Haworth Tompkins. During this time, it will be business as usual in the Theatre with our brilliant programme on offer, and we're taking our Studio programme on Walkabout around Bristol!
Blahblahblah continues our Studio Walkabout Season, inspired by the city-wide partnerships created during the Theatre's first phase of refurbishment in 2011 which saw us stage productions in other venues including Coram Boy with our friends at Colston Hall.
Please note the venue of this show, and we hope you will join us as we stretch our legs across the city and continue to enjoy our brilliant studio programme.
For more information on our redevelopment, click here.


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Author | Pallavi Goorha | Wednesday, Mar 01,2006 8:06 AM
Crayons Communication Dubai, the 100 per cent subsidiary of Crayons Advertising, has added a number of creative accounts to its kitty in the past two months. These include Fujitsu Plasmavision, Super Anti-Septic Disinfectant, Continental Coffee, UAE Wallet and BabyShop.
Commenting on the account wins, Crayons Communication, Agency Head, Arshad Zaheer, said, "I am really happy that the agency has done so well in a short span of seven months. We handle brands from categories as diverse as consumer electronics, FMCG, property, services, retail, etc."
"BabyShop is the largest retail store for children. The account is worth $1 million. Crayons is handling the Child Safety campaign as part of the store's corporate social responsibility programme," Zaheer said.
"Another account that we are handling is Fujitsu Plasmavision, a premium plasma TV manufacturer. Crayons handled the launch of its plasma TVs in the UAE via print ads, which were directly approved by Japan. The account size is $1.5 million. Our main criteria is getting into the niche and the popular segments. We are currently doing print ads for the company as it gives more noticeability here. We will also be doing TVCs in the future," he added.
Crayons is involved in the repositioning exercise of Continental Coffee, a German coffee brand. The account is worth $2 million. "We are targeting the Asian population. We have made a film for them, which will be released in all the channels in the UAE. We have also done packaging and communication for them. We are launching the coffee whiteners for them," Zaheer said.
Another account in the agency's kitty is that of Super Anti-Septic Disinfectant, a British brand, which has been in the market for 20 years. Crayons is involved in repositioning the brand, beginning with its packaging to creating a new TVC. The account size is pegged at $1.5 million.
UAE Exchange of Western Union is another new win for Crayons. The account is worth $2 million. UAE Wallet is money transfer service, which is a big thing in the UAE. The new feature facilitates money transfers via the mobile phone. "UAE Exchange Centre is a foreign exchange and funds transfer/remittances company. We are doing print ads for them," Zaheer said.
"We hope to close this year with $8 million. We have a fantastic creative team headed by Creative Director Jennifer Fernandes. The agency is turning out to be a creative hot shop," he added.
Crayons Communication was started in August 2005 and is headed by Arshad Zaheer. The other clients handled by the agency include Riverside Real Estate property brand in Dubai, processed food brand Neo Care, Creative MP3 players, and Mather Constructions among others.


text:
Human Papilloma Virus (HPV) is accountable for the outbreak of a new deadly disease. It is predicted that this new epidemic, even deadlier than AIDS and will claim many lives.
According to new research, condoms cannot provide 100% protection against HPV (or HIV/AIDS), which can spread through skin-to-skin contact with infected areas of the skin not covered by the condom such as the male and female genitalia.
HPV is the name for a group of viruses that affect your skin and the moist membranes lining your body, for example, in your cervix, anus, mouth and throat.
HPV is a common and highly contagious infection, with over three quarters of $exually active women acquiring it at some time in their lives. HPV is $exually transmitted, but skin-to-skin genital contact is also a well-recognized mode of transmission. This means that condoms cannot give full protection.
HPV is mainly transmitted through $exual contact and most people are infected with HPV shortly after the onset of $exual activity.
Cervical cancer is by far the most common HPV-related disease. Nearly all cases of cervical cancer, which is the leading cause of death in women, can be attributed to HPV infection.
Women are more susceptible to contracting the virus than men.
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MATLAB Newsgroup
Dear all,
In relation with my previous post, it would be enough to solve the following case.
I have a m x n matrix named data:
I have two vectors a and b containing the row and column indexes to access.
Obviously they have the same size
Would it be possible to access the data directly, without a loop?
data=[4 5 3; 5 2 1;4 5 2];
a=[2 1];
b=[3 2];
I'd like to obtain the result in a vector way, result = [1 5]
The length of a and b is normally very long, so the solution result = [data(a(1),b(1)) data(a(2),b(2))], would not be valid
Thank you very much for your help,
On 6/14/2012 5:03 AM, Jose Miguel wrote:
> Dear all,
> In relation with my previous post, it would be enough to solve the following case.
> I have a m x n matrix named data:
> I have two vectors a and b containing the row and column indexes to access.
> Obviously they have the same size
> Would it be possible to access the data directly, without a loop?
> data=[4 5 3; 5 2 1;4 5 2];
> a=[2 1];
> b=[3 2];
> I'd like to obtain the result in a vector way, result = [1 5]
> The length of a and b is normally very long, so the solution
>result = [data(a(1),b(1)) data(a(2),b(2))], would not be valid
> Thank you very much for your help,
> Regards
> Jose
data=[4 5 3; 5 2 1;4 5 2];
a=[2 1];
b=[3 2];
ans =
1 5
Thank you very much for such an elegant solution.
Would it be possible to apply an analogue solution for a 4 dimension variable.
ans =
1 1460 256 512
I want to select the all the data for the 1st and 2nd dimension and 4 sets of data from the 3rd and 4th dimension.
I have four indexes in a vector "a" telling the indexes of the 3rd dimension and another vector with four indexes "b" telling the indexes of the 4th dimension.
The idea is to do:
data = var(:, :, a, b), obviously this instruction doesn't work
Would it be possible to do it with just one access to the data?
The problem of using a loop is that the elapsed time is multiply x 4
Thank you very much,
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In the 4th and final post in the Spotlight on Sun Protection series, we'll take a look at Australia, New Zealand & India and wrap up with some final thoughts on this series and manufacturing sunscreens. If you've just discovered the spotlight on sun protection series, you can click the links to take a look at part 1, where we covered sunscreen regulations in the USA, part 2: the EU and part 3: … [Read more...]
Spotlight on Sun Protection in Asia from In-Cosmetics: part 3
In the past few days we've looked at sunscreen regulations in the USA and EU; today we will examine Asia. As I mentioned in part 1, there is no continuity in regulating sunscreen around the world. As a continent, Asia's various countries have different regulations, SPF ratings and registration requirements and guidelines to bring sunscreens to market. This information was presented by Alain … [Read more...]
In-Cosmetics 2012 Preview
Right now, I'm on the plane to Barcelona reviewing the wealth of information that will be presented on April 17-19 at the In-Cosmetics conference. Here's a preview of what's in store at the largest global platform for cosmetics ingredients this year: The Innovation Zone will host presentations on the latest ingredients, raw materials, technologies and formulation techniques. The most innovative … [Read more...]
Safe Cosmetics Act 2011: Sec. 619 Cosmetic and Ingredient Statements
Kayla has done a fabulous job breaking down the Safe Cosmetics Act of 2011. We are starting at the end of the bill and working our way to the beginning. Here is the post that breaks down SEC 19. Cosmetic and Ingredient Statements. KEY: All Plain Text is directly from H.R. 2359 as it is written and Green is my commentary. ''SEC. 619. COSMETIC AND INGREDIENT STATEMENTS. ''(a) IN … [Read more...]


text:
Global analysis of estrogen receptor beta binding to breast cancer cell genome reveals an extensive interplay with estrogen receptor alpha for target gene regulation
Estrogen receptors alpha (ERα) and beta (ERβ) are transcription factors (TFs) that mediate estrogen signaling and define the hormone-responsive phenotype of breast cancer (BC). The two receptors can be found co-expressed and play specific, often opposite, roles, with ERβ being able to modulate the effects of ERα on gene transcription and cell proliferation. ERβ is frequently lost in BC, where its presence generally correlates with a better prognosis of the disease. The identification of the genomic targets of ERβ in hormone-responsive BC cells is thus a critical step to elucidate the roles of this receptor in estrogen signaling and tumor cell biology.
Expression of full-length ERβ in hormone-responsive, ERα-positive MCF-7 cells resulted in a marked reduction in cell proliferation in response to estrogen and marked effects on the cell transcriptome. By ChIP-Seq we identified 9702 ERβ and 6024 ERα binding sites in estrogen-stimulated cells, comprising sites occupied by either ERβ, ERα or both ER subtypes. A search for TF binding matrices revealed that the majority of the binding sites identified comprise one or more Estrogen Response Element and the remaining show binding matrixes for other TFs known to mediate ER interaction with chromatin by tethering, including AP2, E2F and SP1. Of 921 genes differentially regulated by estrogen in ERβ+
Results indicate that the vast majority of the genomic targets of ERβ can bind also ERα, suggesting that the overall action of ERβ on the genome of hormone-responsive BC cells depends mainly on the relative concentration of both ERs in the cell.
Estrogens are key regulators of cell growth and differentiation in the mammary gland
ERs are able to bind DNA at specific sites in the genome and thereby control gene activity by recruiting transcriptional mediators and co-regulators, as well as a host of other nuclear proteins with different roles in ER-mediated control of gene activity
Although the two receptors are quite similar in sequence and structure, in BC ERβ has considerably different biological effects than ERα
Gene expression studies performed in BC cell lines expressing endogenous ERα and recombinant ERβ
Recently, next-generation sequencing technologies combining chromatin immunoprecipitation (ChIP) either with genomic DNA hybridization to microarrays (ChIP-on-chip) or massively parallel sequencing (ChIP-Seq, ChIP-PET), opened new venues for our understanding of physical and functional associations between transcription factors and chromatin
Results and Discussion
Establishment and characterization of ERβ-expressing MCF-7 cells
Stabilized human BC cell lines expressing endogenous ERα and ERβ at comparable levels are not available. For this reason, we first generated and characterized cell lines derived from ERα-positive MCF-7 cells expressing full-length human ERβ (ERβ1) at levels similar to those of endogenous ERα. This strategy was adopted to prevent artefacts due to ERβ over-expression in the cell and to mimic what observed in primary breast tumors, where very high expression of this receptor has never been observed. As suitable antibodies for efficient immunoprecipitation of chromatin-bound ERβ are not available, the expressed proteins were tagged on either their C- (Ct-ERβ) or N-terminus (Nt-ERβ) with the TAP epitope. This approach allows to track tagged ERs in different cell compartments and to efficiently immunoprecipitate and purify them
Figure 1
Functional characterization of ERβ-expressing MCF-7 cells
Functional characterization of ERβ-expressing MCF-7 cells. (A) Nuclear translocation of ERα and ERβ shown by western blot analysis on cytosolic (c) and nuclear (n) protein extracts, prepared from
Effects of ERβ on the estrogen-responsive MCF-7 cell transcriptome
Expression of ERβ is known to cause significant changes in the genomic response to estrogen in target cells. To identify the genes whose estrogen regulation in hormone-responsive BC cells is perturbed by ERβ, we performed gene expression profiling with microarrays in estrogen-starved, quiescent
Figure 2
Gene expression differences in absence or presence of ERβ
Gene expression differences in absence or presence of ERβ.
Global mapping of ERα and ERβ binding to MCF-7 cell genome
The widespread effects of ERβ on MCF-7 cell transcriptome are likely to result from multiple effects of this receptor in the cells, including direct regulation of primary response genes
Additional File 1
Grober_ER-alpha_Binding_Sites ERα binding sites. The UCSC genome BED formatted file lists the chromosome, start coordinate, stop coordinate and identifier of the ERα binding sites.
Click here for file
Additional File 2
Grober_ER-beta_Binding_Sites ERβ binding sites. The UCSC genome BED formatted lists the chromosome, start coordinate, stop coordinate and identifier of the ERβ binding sites.
Click here for file
Figure 3
Sequence analysis of ERα, ERβ and ERα+ERβ binding sites
Sequence analysis of ERα, ERβ and ERα+ERβ binding sites. (A) Venn diagram showing a summary of ERα and ERβ binding sites identified in TAP-ERβ cells by ChIP-Seq. (B) Classification of ERα and β binding sites based on the presence of a perfectly or imperfectly palindromic Estrogen Response Element (ERE, green), an ERE hemipalindrome (hERE, blue) or no ERE (none, red). (C) ERE motif matrices identified in each of the three ER binding regions indicated (
We then examined the ERE- sequences to search for enriched binding motifs for other transcription factors that might play a role in ER binding to chromatin in the absence of canonical EREs. ERs are known to be able to bind chromatin indirectly, by physically interacting with DNA-bound TFs (tethering), including SP1
Additional File 3
Sheet 1:
Click here for file
Identification of primary ERβ target genes
To identify the genes directly controlled by ERβ binding to the genome, and analyze the interplay between the two ERs in gene regulation, we combined the ChIP-Seq data with those relative to estrogen responsive genes differentially regulated by E2 in ERβ+
To identify among the genes differentially regulated by estrogen in ERβ+
Figure 4
Putative ERβ primary targets
Putative ERβ primary targets.
A functional analysis of the primary ERβ target genes identified here with Ontologizer
Additional File 4
GO analysis of primary ERβ target genes. Containing the following information: Biological process, Gene Ontology term, Name, Count in total GO population, Count in selected genes, % genes and p-value.
Click here for file
Additional File 5
GO analysis of primary ERα target genes. Containing the following information: Biological process, Gene Ontology term, Name, Count in total GO population, Count in selected genes, % genes and p-value.
Click here for file
Among the RNAs encoded in the genome, microRNAs (miRNAs) have emerged as master regulators of gene expression for their ability to influence mRNA concentration and activity by post-transcriptional mechanisms. Recent results highlighted the role of miRNA in BC cells response to estrogen
Additional File 6
ERβ binding sites in proximity of miRNA loci. Containing the following information: miRNA name, ID of ER-β binding site upstream, Distance from the closest ERβ binding site upstream, ID of ER-β binding site downstream and Distance from the closest ERβ binding site downstream.
Click here for file
We tested the hypothesis that the observed distribution of sites near the pre-miRNA loci occurred at random by applying a bootstrap approach. To this end, we repeatedly sampled 1000 times the same number of sites of the real ERβ binding set, with the same length distribution, a similar distribution among chromosomes but randomly selected coordinates. We then counted the number of pre-miRNA loci and the number of randomly generated sites found within 10 kb of each other and compared their distributions with that of the experimentally detected ones. The number of randomly generated sites within 10 kb of a pre-miRNA never reached the value detected experimentally, while the number of miRNA loci scoring an artificial site in close proximity was equal or above what measured only in 7.6% of the cases. These results can be explained also by the observation that in several cases ERβ binds both up- and down-stream of the pre-miR locus, a result that support a functional significance of this observation. In fact, the ratio between the number of ERβ binding sites and the number of pre-miRNA loci within 10 kb of each other is 1.5, while this varied between 0.5 and 1 for the randomly generated sets (data not shown). We thus conclude that although some of the ERβ sites detected in or near pre-miRNA loci may be the result of a random, non functional event, these is likely to represent rare events, as random distribution never reaches the enrichment level observed experimentally. Indeed, preliminary miRNA profiling analyses carried out in
ERβ binding to the mitochondrial genome
Mitochondrial DNA (mtDNA) is usually overlooked in whole-genome ChIP-seq analyses, since identification of enriched peaks is more difficult here due to a much higher noise, consequence of the high and variable number of mtDNA copies in the cell. ERβ has been shown to localize in the mitochondria in different cell types
Figure 5
Mitochondrial ER-beta binding sites
Mitochondrial ER-beta binding sites. (A) Genome Browser view of the ERβ binding site identified in mitochondrial genome. (B) Validation of ERβ binding to mitochondrial DNA by ChIP. Results shown are representative of duplicate analyses. E2: 10-8M 17β-estradiol; PPT: 10-8M 1,3,5-tris(4-hydroxyphenyl)-4-propyl-1H-pyrazole (selective ERα agonist); DPN: 10-8M 2,3-bis(4-hydroxyphenyl) propionitrile (ERβ agonist). (C) Western blot analysis of ERβ and/or ERα in purified mitochondria from Ct-ERβ and Ct-ERα
The results of this study indicate that
Plasmid preparation, cell lines, cell culture and stable transfections
Different BC cell lines were used: MCF-7 TET Off (ER-alpha positive; ATCC, Cat No. HTB-22) and SKBR3 (ER-alpha negative; ATCC, Cat No. HTB-30). MCF-7 TET Off cells (described here as
Preparation of cell extracts, mitochondria isolation and immunoblotting analyses
Cells starved in 100-mm dishes were stimulated for 45 minutes, harvested in cold PBS and collected by centrifugation at 1000 × g. The cell pellets were then resuspended in three volumes of Hypotonic Buffer (HB) (20 mM HEPES pH 7.4, 5 mM Sodium Fluoride, 10 μM Sodium Molybdate, 0.1 mM EDTA, 1 mM dithiothreitol, 1 mM protease inhibitors, 1 mM Phenylmethyl-Sulfonyl Fluoride). Cells were then incubated on ice for 15 min and 0.5% Nonidet P-40 followed by spinning 30 sec at 4°C at 13000 × g. Supernatants were recovered and clarified at 13,000 × g for 15 min at 4°C while pellets were resuspended in hypotonic buffer, stratified on 25% sucrose-HB solution and centrifuged at 6000 × g for 15 min at 4°C. The resulting pellets were then resuspended in one volume of Nuclear Lysis Buffer
Mitochondria were isolated from 20 × 106 Ct-ERβ or Ct-ERα cells (in 150 mm culture dishes) as described
Homogenate, cytosol, nuclear or mitochondrial extracts from equivalent cell number were fractionated by SDS-PAGE. Immunoblot analysis was performed using the following primary antibodies: Ct-ERα (HC-20; sc-543) from Santa Cruz Biotechnology, Inc., TAP tag (CAB1001) from Open Biosystems, α-Tubulin (T 6199) from Sigma-Aldrich, ATPase B (ab14730) from Abcam. Peroxidase-labelled anti-rabbit or -mouse immunoglobulin antisera were used according to the manufacturer's instructions (Amersham Italia).
Transient Transfections and Luciferase Assay
Wild type MCF-7, Ct-ERβ and Nt-ERβ clones were starved for 5 days in estrogen-free medium. Then 5 × 105 cells/dish were seeded in 60 mm culture dishes and transfected by using 25 μg/dish polyethylenimine (Polysciences, Inc.) with 2.5 μg/dish DNA, including 300 ng ERE-tk-Luc
SKBR3 cells were grown to 60-70% confluence and transfected with Lipofectamine 2000 reagent (Invitrogen) and OPTI-MEM (Invitrogen) according to the manufacturer's instructions. The plasmids used were pSG5-ERβ, encoding full-length ERβ (ERβ1), pSG-HEGO, encoding the full-length ERα and the corresponding pSG5 empty vector (Stratagene), pUSE-C-TAP-ERβ and pUSE-N-TAP-ERβ, encoding full-length ERβ tagged, respectively, at the C-term and at the N-term
Cell Cycle Analysis
Estrogen-deprivation was always controlled by cell cycle analysis as follows. Cells (1.5 × 105 cells/dish) were starved in 60 mm culture dishes, stimulated for 27 hrs and collected in PBS containing 50 μg/ml propidium iodide, 0.1% (v/v) sodium citrate, 0.1% (v/v) Nonidet P-40. Cell samples were incubated in the dark for at least 15 min at room temperature, or overnight at 4°C, and analyzed by a FACScalibur flow cytometer using the CellQuest software package (BD Biosciences), according to standard protocols suggested by the manufacturer
Cell Proliferation Assay
Hormone-starved cells (3000/well) were seeded in 96-well dishes. After 12 hrs, medium was changed to include the indicated compounds. After appropriate stimulation, cells were washed in phosphate-buffered saline (PBS) and fixed with 12.5% glutaraldehyde for 20 min at room temperature, followed by washing with distilled water, incubation with 0.05% methylene blue for 30 min, rinsing and incubation with 0.33 M HCl for 18 hrs. Absorption was measured at 620 nm.
RNA purification
Total RNA was extracted from
In each case RNA derived from two independent experiments performed in duplicate was used. Before use, RNA concentration in each sample was assayed with a ND-1000 spectrophotometer (NanoDrop) and its quality assessed with the Agilent 2100 Bioanalyzer with Agilent RNA 6000 nanokit (Agilent Technologies).
Microarray analyses
Total RNA extracted from Ct-ERβ and Nt-ERβ cells were pooled. For mRNA expression profiling, 500 ng total RNA were reverse transcribed, as described previously
Chromatin Immunoprecipitation
Ct-ERβ and Nt-ERβ cells were hormone-deprived for 4 days and chromatin was extracted in several replicates before (-E2) and after stimulation for 45 minutes with 10-8M 17β-estradiol (+E2) or, where indicated, with the 10-8M selective ERα agonist 1,3,5-tris(4-hydroxyphenyl)-4-propyl-1H-pyrazole (PPT) or 10-8M ERβ agonist 2,3-bis(4-hydroxyphenyl) propionitrile (DPN), from Tocris Cookson.
Chromatin was prepared with the Millipore/Upstate Chromatin Immunoprecipitation (ChIP) Assay Kit (Millipore) according to the instruction provided by the producer, using a variation of the protocol described at the Upstate website. For each assay, a total of 5 × 106 cells were fixed with 1% formaldeyde for 10 min at room temperature, the reaction was then stopped by adding glycine at final concentration of 0.125 M. Fixed cells were washed twice with ice-cold PBS, harvested by scraping, centrifuged and the cell pellets were re-suspended in SDS lysis buffer. Samples were sonicated with a Diagenode Bioruptor (Diagenode) for 12 cycles of 30 sec at high power, centrifuged at 12500 xg for 15 minutes and diluted 8-fold in ChIP dilution buffer. After removing an aliquot (whole-cell extract input), the chromatin sample was divided in three aliquots, that were incubated at 4°C overnight with antibodies against either the C-term (HC-20, from Santa Cruz Biotechnology) or N-term (anti-Estrogen Receptor 18-32, from SigmaAldrich) of human ERα or with IgG Sepharose 6 fast Flow (GE Healthcare Bio-Science AB) for TAP-ERβ
Primers for ChIP-QPCR validation of the mitochondrial genome ERβ binding site were the following: 5'-GATCACAGGTCTATCACCCTATTAACC (forward) and 5'-CAGCGTCTCGCAATGCTATC (reverse).
Samples preparation for ChIP-Seq
DNAs from Ct-ERβ and Nt-ERβ cells treated with E2 were pooled together to generate an ERβ +E2 sample and the same was done for DNAs from hormone-starved cells (ERβ -E2 sample). Similarly, IPP DNAs obtained with anti-C-term and anti-N-term ERα Abs were pooled together to generate ERα +E2 and ERα -E2 samples. About 20 ng of ChIP DNA was purified using the MinElute PCR Purification Kit (QIAGEN, Italy), with a recovery of 55-70%, as assessed with the Quant-IT DNA Assay Kit-High Sensitivity and a Qubit Fluorometer (Invitrogen). Preparation of IPP DNA libraries for massively parallel sequencing was performed from 10 ng purified DNA according to the Illumina ChIP-Seq sample preparation kit protocol (Illumina Inc.). Libraries were sequenced with the Illumina Cluster Station and Genome Analyzer II according to manufacturer's instructions.
ChIP-Seq data analysis
The sequence tags generated by massively parallel sequencing were aligned on the human genome (hg18) with the software ELAND, allowing up to 2 mismatches. Enriched regions from ERα +E2 and ERβ +E2 samples were compared with the same from ERα -E2 and ERβ -E2 samples, respectively. The enriched ChIP-Seq peaks were identified using MACS (Model-based Analysis of ChIP-Seq) version 220.127.116.11
Computational searches for ERE sequences
The ERE binding motif was searched in binding sites with the MatInspector application
TFBS over-representation analysis
ERE- sequences were analyzed to search for known TF binding sites (TFBSs) that were enriched (over-represented) with respect to their relative frequency in the whole human genome. This analysis was performed with the RegionMiner application of the Genomatix software suite
Classification of ERβ binding sites and identification of primary ERβ responsive genes by combining ChIP-Seq and expression profiling data
Three sets of ER binding sites were taken in consideration. The first and second comprised, respectively, the ERβ and ERα sites mapped in TAP-ERβ cells and the third (named 'MCF-7 ERα') included all ERα binding sites identified in
Gene Ontology analysis
To identify Biological Process GO terms statistically overrepresented in our regulated gene lists, we used Ontologizer 2.0, a tool for GO term enrichment analysis of genes derived from an experiment
Microarray and ChIP-Seq data accession numbers
The microarray and ChIP-Seq data have been deposited in the Array Express database ( HYPERLINK "
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
OMVG, MM, GG, MR, LC, MRDF, LF, GN, MFP, OP and RT participated in the conception and design of the study, performed
Work supported by the European Union (CRESCENDO I.P., contract n.er LSHM-CT2005-018652), the Italian Association for Cancer Research (grant IG-8586) and the Italian Ministry for Education, University and Research (grant PRIN 2008CJ4SYW_004), Fondazione per il Sud (grant 2009-PdP-22) to AW. MR, GN and RT are recipient of fellowships from Fondazione per il Sud. The authors thank Dr Rosario Casale for technical assistance and Francesca Rizzo for revision of the manuscript.
Estrogen receptors: how do they signal and what are their targets
The multifaceted mechanisms of estradiol and estrogen receptor signaling
Breast cancer, estrogen receptor and ligands
Nuclear receptors: genomic and non-genomic effects converge
A large set of estrogen receptor beta-interacting proteins identified by tandem affinity purification in hormoneresponsive human breast cancer cell nuclei
Identification of proteins associated with ligand-activated estrogen receptor alpha in human breast cancer cell nuclei by tandem affinity purification and nanoLC-MS/MS
Estrogen signaling: a subtle balance between ER alpha and ER beta
Estrogen response element-dependent regulation of transcriptional activation of estrogen receptors alpha and beta by coactivators and corepressors
Estrogen receptors alpha and beta form heterodimers on DNA
Estrogen receptor beta2 negatively regulates the transactivation of estrogen receptor alpha in human breast cancer cells
Comparative analysis of nuclear estrogen receptor alpha and beta interactomes in breast cancer cells
Estrogen receptor (ER) beta modulates ERalpha-mediated transcriptional activation by altering the recruitment of c-Fos and c-Jun to estrogen-responsive promoters
Estrogen receptors: selective ligands, partners, and distinctive pharmacology
Estrogen receptor beta acts as a dominant regulator of estrogen signaling
Expression of estrogen receptor beta wild-type and its variant ERbetacx/beta2 is correlated with better prognosis in breast cancer
Clinical significance of estrogen receptor β in breast cancer
Estrogen Receptor (ER)-beta Reduces ERalpha-Regulated Gene Transcription, Supporting a "Ying Yang" Relationship between ERalpha and ERbeta in Mice
ER beta Inhibits Proliferation and Invasion of Breast Cancer Cells
Loss of tumourigenicity of stably ERbeta-transfected MCF-7 breast cancer cells
Estrogen Receptor Inhibits Human Breast Cancer Cell Proliferation and Tumor Formation by Causing a G2 Cell Cycle Arrest
Impact of estrogen receptor beta on gene networks regulated by estrogen receptor alpha in breast cancer cells
Inhibitory effects of estrogen receptor beta on specific hormone-responsive gene expression and association with disease outcome in primary breast cancer
A genome-wide study of the repressive effects of estrogen receptor beta on estrogen receptor alpha signaling in breast cancer cells
Biological effects of ERalpha- and ERbeta-selective estrogens
Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1
Estrogen Receptor alpha Controls a Gene Network in Luminal-Like Breast Cancer Cells Comprising Multiple Transcription Factors and MicroRNAs
An oestrogen-receptor-alpha-bound human chromatin interactome
Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen
Estrogen receptor beta binds to and regulates three distinct classes of target genes
Genome-wide dynamics of chromatin binding of estrogen receptors alpha and beta: mutual restriction and competitive site selection
Genome-wide mapping of estrogen receptor-beta-binding regions reveals extensive cross-talk with transcription factor activator protein-1
The genome landscape of ERalpha- and ERbeta-binding DNA regions
Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei
Estrogens and progesterone promote persistent CCND1 gene activation during G1 by inducing transcriptional derepression via c-Jun/c-Fos/estrogen receptor (progesterone receptor) complex assembly to a distal regulatory element and recruitment of cyclin D1 to its own gene promoter
Selective estrogen receptor modulators: discrimination of agonistic versus antagonistic activities by gene expression profiling in breast cancer cells
Profiling of estrogen up- and down-regulated gene expression in human breast cancer cells: insights into gene networks and pathways underlying estrogenic control of proliferation and cell phenotype
Convergent transcriptional profiles induced by endogenous estrogen and distinct xenoestrogens in breast cancer cells
ChIP-seq: welcome to the new frontier
Model-based analysis of ChIP-Seq (MACS)
Fitting a mixture model by expectation maximization to discover motifs in biopolymers
JASPAR 2010: the greatly expanded open-access database of transcription factor binding profiles
DNA familial binding profiles made easy: comparison of various motif alignment and clustering strategies
Non-classical genomic estrogen receptor (ER)/specificity protein and ER/activating protein-1 signaling pathways
Transcriptional activation of transforming growth factor alpha by estradiol: requirement for both a GC-rich site and an estrogen response element half-site
The estrogen receptor enhances AP-1 activity by two distinct mechanisms with different requirements for receptor transactivation functions
Estrogen receptor (ER)-beta isoforms: a key to understanding ER-beta signaling
An integrated workflow for analysis of ChIP-chip data
CTCF gene mutations in invasive ductal breast cancer
Estradiol stimulates transcription of nuclear respiratory factor-1 and increases mitochondrial biogenesis
Ga-ga over GAGA factor
Genomewide analysis of Drosophila GAGA factor target genes reveals context-dependent DNA binding
Ontologizer 2.0--a multifunctional tool for GO term enrichment analysis and data exploration
Estrogen receptor {beta}1 expression is regulated by miR-92 in breast cancer
Estradiol-regulated microRNAs control estradiol response in breast cancer cells
The estrogen receptor-alpha-induced microRNA signature regulates itself and its transcriptional response
MicroRNA Cluster 221-222 and Estrogen Receptor {alpha} Interactions in Breast Cancer
Widespread estrogen-dependent repression of micrornas involved in breast tumor cell growth
Estradiol downregulates miR-21 expression and increases miR-21 target gene expression in MCF-7 breast cancer cells
Involvement of microRNAs in breast cancer
MicroRNA gene expression deregulation in human breast cancer
MicroRNA profiling as a tool to understand prognosis, therapy response and resistance in breast cancer
Mitochondrial localization of estrogen receptor beta
Binding of MCF-7 cell mitochondrial proteins and recombinant human estrogen receptors alpha and beta to human mitochondrial DNA estrogen response elements
ERbeta shifts from mitochondria to nucleus during estrogen-induced neoplastic transformation of human breast epithelial cells and is involved in estrogen-induced synthesis of mitochondrial respiratory chain proteins
Estrogen-induced G1/S transition of G0-arrested estrogen-dependent breast cancer cells is regulated by mitochondrial oxidant signaling
Estrogen actions on mitochondria--physiological and pathological implications
Ligand-dependent repression of the erythroid transcription factor GATA-1 by the estrogen receptor
HMGA1 levels influence mitochondrial function and mitochondrial DNA repair efficiency
The functional interaction between HMGA1 and the estrogen receptor requires either the N- or the C-terminal domain of the receptor
Human estrogen receptor beta binds DNA in a manner similar to and dimerizes with estrogen receptor alpha
Mouse estrogen receptor beta forms estrogen response element-binding heterodimers with estrogen receptor alpha
Single-chain estrogen receptors (ERs) reveal that the ERalpha/beta heterodimer emulates functions of the ERalpha dimer in genomic estrogen signaling pathways
A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells
Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts
Distinct signaling pathways mediate stimulation of cell cycle progression and prevention of apoptotic cell death by estrogen in rat pituitary tumor PR1 cells
17beta-estradiol inhibits forskolin-induced vascular endothelial growth factor promoter in MCF-7 breast adenocarcinoma cells
Identification of genes involved in growth inhibition of breast cancer cells transduced with estrogen receptor
17 beta-Estradiol overcomes a G1 block induced by HMG-CoA reductase inhibitors and fosters cell cycle progression without inducing ERK-1 and -2 MAP kinases activation
A genomic view of estrogen actions in human breast cancer cells by expression profiling of the hormone-responsive transcriptome
Comparative gene expression profiling reveals partially overlapping but distinct genomic actions of different antiestrogens in human breast cancer cells
Quantitative expression profiling of highly degraded RNA from formalin-fixed, paraffin-embedded breast tumor biopsies by oligonucleotide microarrays
MatInspector and beyond: promoter analysis based on transcription factor binding sites
oPOSSUM: identification of over-represented transcription factor binding sites in co-expressed genes
TM4: a free, open-source system for microarray data management and analysis
The UCSC Genome Browser Database: update 2009


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found a new home!
Plenty of my friends are looking
for one too. Find a pet to adopt.
I am good with dogs and good with cats.
My name is Dozer! I know it sounds kinda silly but it suits me quite well as I am just a happy go lucky, carefree, all four left paws pup … especially now that I am with CAA Rescue
All the volunteers are a little baffled how a 12 week old puppy like me could be walking the streets as a stray and then held at the local gas kill pound for 5 long days in a dirty cage waiting for someone to come claim me … but no one ever did. I guess that is what makes the volunteers at CAA Rescue look at me with those sad eyes!!! When I see them sad though I quickly cheer them up with my happy puppy tricks of knocking everything over in sight, tearing through the house like a mini bull dozer (… ahhahaa see now you get it) and coming up to them for cuddles and kisses! I especially like the cuddles and kisses!!!
There are blank slates and then there is ME!! At such a young age you don't expect me to be dealing cards or anything but there should be some signs that I have been taught some basic obedience at this point and time … but nope! NADA! Or if I was taught it was not in any language my foster family knows.
I need someone who will spend the time training me! I am a super uber duper smart boy, foster mummy started clicker training with me on the first night I came into her home and WHAM I got it right away and started responding! I am very treat motivated so I am highly trainable and very attentive especially if you have those yummy freeze dried liver treats in your pocketses
I am still learning that business should be done outside… while I do, do it when I am outside I am still learning all the rules of the house. Foster mummy told me not to worry as she cleaned up my number 1 this morning; she said I was a smart boy and that with a loving and patient home I will learn in no time where da biz is to be done. I think my foster mummy is pretty darn awesome and I hope my next mummy will be as patient and kind and caring. It is kind of hard for me this time of year as I am little and with such a short coat I get cold really fast so being outside in chilly temperatures is really not my favorite thing.
Do NOT even ask about the leash training, we are so totally not there yet… as I mentioned I am a bit a goof ball with my long lanky legs stumbling through everything so right now foster mummy is focusing on basic obedience and house training and she says she is leaving my new family with the fun stuff to show me like walks on the leash, chasing balls and bringing them back, meeting new doggies and being polite with introductions!
I travel super well in the car with no signs of anxiety
To bring me home there will be adoption fee and a sterilization deposit fee. The adoption fee covers all my vetting: base vaccinations including rabies, deworming, neuter and micro chip at Animal 911 vet in Roxboro; my new family will have to make the remaining visits. The sterilization deposit will be returned on the day that I get neutered at Animal 911. CAA Rescue is asking for this sterilization deposit to protect me and my future, to ensure I am happy, safe and healthy for all time. So if you have puppy training knowledge, are a local family and would like to meet me in hopes of taking me home forever when I am ready to make the next big step in my life please email email@example.com and ask them for an application
Breed: Labrador mix with (…….honestly we have no idea………. sorry folks…) possible Dane
Sex: Male
Housetrained: no, a work in progress
Leash trained: no, a work in progress
Age: est DOB Aug 25 2014
Foster Location: Valleyfield, Quebec
Anxiety: none to note in foster care
Vet History: November 19th health check, 1st vaccinations and deworming
Future Visits at our Vet Animal 911 in Roxboro that adoptive family will be required to make
Week of December 15th: 2nd vaccinations and deworming
Week of January 12th: 3rd and final vaccinations and deworming
Week of February 23rd: neuter and micro chip
Adoption Fee and Sterilization Deposit Apply
If you would like to know more about Dozer pls email us email@example.com
*pls note that not all messages sent to us directly through this site are received so best to email us directly at firstname.lastname@example.com
Please visit us on facebook https://www.facebook.com/CompassionateAnimalAdoption
Dozer, a Pierrefonds Labrador Retriever/Great Dane puppy was adopted! Here's how you can benefit by looking at adoption as an option to buying a Labrador Retriever/Great Dane for sale in Pierrefonds, or seeking a Labrador Retriever/Great Dane puppy from a breeder in Pierrefonds.


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DaVita Inc. (NYSE: DVA), is firmly in the large market cap category with market cap of 13137.92. To be considered a large market cap stock companies have to be valued at more than $10 billion. The current price for DaVita Inc. is 62.81 (with a change from open of 0.82%), this in conjunction with a target price of 78.2. Volume today has so far reached 538599, with an average volume of about 1768.83. The GAP for this stock, is -0.57%.
The P/E ratio is 37.01, bringing the forward P/E to a value of 15.13. The current EPS for DaVita Inc., is 1.69, with a EPS growth for this year coming to a value of -62.60%. Performance has been interesting with performance year to date coming to -10.11%.
Looking further into the growth statistics for DaVita Inc., EPS is looking like it will grow 10.13% in the next year. Looking even further it is projected to be 13.50%. Quarter over quarter is -67.20% for EPS growth and for sales growth quarter over quarter it is 8.20%. Looking at past EPS growth statistics we see that for the past five years it has been -8.60%. Sales growth for the past five years has in fact been 17.20%.
Looking at the ownership, we see that the insider ownership is at 0.10% and institutional ownership is 86.50%. Shares outstanding is 209.67, while shares float is at a value of 206. Float short for DaVita Inc. is 1.81% and the short ratio is 2.1.
In terms of debt, long term debt/equity is 1.89, while the total debt/equity comes to 1.92. The ability to meet these debts, means that the current ratio is 1.7, and the quick ratio is 1.6. The most recent gross margin is 28.50% and their operating margin is 10.10%. The profit margin is 2.50%.
DaVita Inc. has seen returns on assets of 1.90%, and on investment, 6.20%. Return on equity is 7.50%. Price/Earnings to growth ratio is 2.74. Price/Sales ratio is at a value of 0.92, with Price/book ratio coming to 2.7 and Price/cash is 7.83, and finally Price/Free cash Flow is 10.22.
The simple moving averages are -5.15% and -11.42% for 20 day and 200 day simple moving average respectively. For the 52 week high and low the 52 week high is -20.43% and the 52 week low is 2.36%.
DaVita Inc. has a dividend yield of *TBA, with a current price of 62.81. The ability to pay this means the payout ratio is 0.00%. This is in an industry, Specialized Health Services, with equally high dividends from other companies in this sector, which it is Healthcare. DaVita Inc. is based in USA.
Performance in the Specialized Health Services industry means that DaVita Inc. has seen performance for the past week of -2.26%, with performance for the month coming to -2.02%. Looking at the data for the quarter it is at a value of -19.39%, and for the half year the performance is -14.57%. Volatility for this week is 1.85% and for the month this is 1.65%.
Disclaimer: Remember there is a risk to your investment, this is not a recommendation, nor personal advice, never invest more than you are able too loose.


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login with
Post c-section pregnancy with a terrible abdominal pain
Any terrible pain needs to be reported to your doctor right away. Please call your doctor or go to the hospital. It is the safest thing to get checked out and be sure nothing serious is going on. Terrible abdominal pain can be a symptom of a number of serious complications and needs to be evaluated.
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About Katie
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On Moms.com since: Jan 21, 2014
***Community Manager of Moms.com*** Mom to four amazing children. I am passionate about helping and supporting new moms as they enter into motherhood. I am a certified doula, certified childbirth educator and an accredited breastfeeding counselor.


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People's United FinancialDividend Yield: 4.40% People's United Financial (NASDAQ: PBCT) shares currently have a dividend yield of 4.40%. People's United Financial, Inc. operates as the bank holding company for People's United Bank that provides commercial banking, retail and business banking, and wealth management services to individual, corporate, and municipal customers. The company has a P/E ratio of 19.29. The average volume for People's United Financial has been 3,511,000 shares per day over the past 30 days. People's United Financial has a market cap of $4.7 billion and is part of the banking industry. Shares are down 1.8% year-to-date as of the close of trading on Friday. TheStreet Ratings rates People's United Financial as a buy. The company's strengths can be seen in multiple areas, such as its increase in stock price during the past year, growth in earnings per share, reasonable valuation levels, expanding profit margins and notable return on equity. We feel these strengths outweigh the fact that the company has had sub par growth in net income. Highlights from the ratings report include:
- The stock has risen over the past year as investors have generally rewarded the company for its earnings growth and other positive factors like the ones we have cited in this report. Turning our attention to the future direction of the stock, it goes without saying that even the best stocks can fall in an overall down market. However, in any other environment, this stock still has good upside potential despite the fact that it has already risen in the past year.
- PEOPLE'S UNITED FINL INC has improved earnings per share by 5.5% in the most recent quarter compared to the same quarter a year ago. The company has demonstrated a pattern of positive earnings per share growth over the past two years. We feel that this trend should continue. During the past fiscal year, PEOPLE'S UNITED FINL INC increased its bottom line by earning $0.74 versus $0.72 in the prior year. This year, the market expects an improvement in earnings ($0.87 versus $0.74).
- The gross profit margin for PEOPLE'S UNITED FINL INC is currently very high, coming in at 88.46%. It has increased from the same quarter the previous year. Despite the strong results of the gross profit margin, PBCT's net profit margin of 17.77% significantly trails the industry average.
- The revenue fell significantly faster than the industry average of 106.4%. Since the same quarter one year prior, revenues slightly dropped by 0.8%. The declining revenue has not hurt the company's bottom line, with increasing earnings per share.
- You can view the full People's United Financial Ratings Report.
- Our dividend calendar.


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Voyeur : November 2008
SPECIAL 1 1 :: MIO MOOV 370 2 4 Moov's pre-installed live traffic update software lets you receive real-time traffic reports, alerts you to congestion and accidents and, in Sydney, Melbourne and Brisbane, gets the scoop on roadworks and closures. Net connectivitymeans you can also locate restaurants, petrol stations and more. PRICE :: $549 VISIT :: www.mio.com.au 2 :: NAVMAN S300T PLATINUM Navman's new slimmer, lightweight S-series is easy to carry if you need GPS navigation on foot, and comes with an in-built video player and FM radio. As well as speed camera alerts, the Platinumwarns of school zones, railway crossings and more. PRICE :: $699 VISIT :: www.navman.com.au 3 :: TOMTOM GO 930 3 Getting lost in transit is difficult with the GO 930. It includesmaps for 45 countries and a huge database of hotels, restaurants and attractions. Back home, it provides speed limits for the roads you're on, alerts you to fixed speed cameras and toll roads and even gives you estimated times of arrival. PRICE :: $849 VISIT :: www.tomtom.com 4 :: GARMIN NÜVI 255W The nüvi gives you turn-by-turn spoken directions or can be set to speak street names. Choose from2D or 3Dmaps and a 'Where amI?' button showing nearby hospitals, police stations and petrol stations. Reasonably priced, it's slim and light, so it's a cinch to carry. PRICE :: $399 VISIT :: www.garmin.com GPS 136 VIRGINBLUE WHO NEEDS MAPS AND STREET DIRECTORIES WHEN YOU CAN GET AROUND WITH THESE COOL NAVIGATORS? UNITS
December 2008
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Anniversary Sale
Articles of Society Mya Skinny Jeans (Glendale)
Sale: $38.90
After Sale: $59.00
CITIZENS OF HUMANITY Rocket High Waist Skinny Jeans
A medium-blue wash is perfectly versatile in figure-shaping high-rise skinny jeans cut in a supersoft denim blend. Understated whiskering and fading lend the look of organic wear, while mildly distressed pockets suggest this is the pair you reach for time and time again. Color(s): albion. Brand: CITIZENS OF HUMANITY. Style Name:Citizens Of ... Learn More
DL1961 Florence Instasculpt Skinny Jeans
Figure-hugging skinny jeans instantly sculpt the waist, bottom and legs while providing comfort and shape retention. Subtle whiskering and fading add dimension and lived-in authenticity to this versatile style. Color(s): carry-on. Brand: DL 1961. Style Name:Dl1961 Florence Instasculpt Skinny Jeans (Carry-On). Style Number: 5391285. Available in stores. Learn More
FRAME Distressed Release Hem Skinny Jeans
London-inspired style combined with the quality of the LA denim industry defines fresh go-to jeans styled with trend-right released hems, edgy rips at the knees, and supersoft and stretchy tech denim that offers a flattering fit. Color(s): lilac blanc, lilac film noir. Brand: FRAME DENIM. Style Name:Frame Distressed Release Hem ... Learn More
FRAME Forever Karlie Skinny Jeans
Ditch the flood pants. LA-based Frame Denim has teamed up with supermodel Karlie Kloss to create a pair of jeans for long-limbed ladies. Inspired by London fashion and crafted in the designer denim capital of the world, these chic skinnies flatter taller figures with a higher rise, elongated proportions and ... Learn More
HUDSON JEANS Collin Crop Skinny Jeans
Whiskering and hand-sanded fading accentuate the curve-sculpting silhouette of supersoft jeans cut in an ankle-skimming silhouette. Hudson's signature stretch denim provides an incredibly soft texture and fantastic shape retention. Color(s): light azure. Brand: HUDSON. Style Name:Hudson Jeans Collin Crop Skinny Jeans (Light Azure). Style Number: 5321230. Available in stores. Learn More
INGRID & ISABEL® Sasha Maternity Skinny Jeans with
Keep on trend even while growing a tiny human with these distressed skinny jeans featuring an innovative Crossover Panel that helps them stay up while providing enhanced lower-back support. Color(s): distressed light indigo. Brand: Ingrid & Isabel. Style Name:Ingrid & Isabel Sasha Maternity Skinny Jeans With Crossover Panel. Style Number: ... Learn More
PAIGE Denim Hoxton High Waist Ultra Skinny Jeans
Faded high-rise jeans touched up with light whiskering and distressing and cut for a curve-defining silhouette are comfortable and versatile in equal measure. Color(s): big sur. Brand: PAIGE. Style Name:Paige Denim Hoxton High Waist Ultra Skinny Jeans (Big Sur). Style Number: 5233017. Available in stores. Learn More
PAIGE Legacy - Hoxton Ultra Skinny Jeans
Lightly distressed and darkly washed skinny jeans with a vintage-inspired high rise are the perfect wardrobe mainstay. Signature LEGACY fabric gives this streamlined pair superior elasticity for all-day comfort and support. Color(s): donna destructed. Brand: PAIGE. Style Name:Paige Legacy - Hoxton Ultra Skinny Jeans (Donna Destructed). Style Number: 5332287. Available ... Learn More


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|Charlotte of York
|Duchess of Pembroke (more...)
| William Cecil, 3rd Duke of Pembroke
m. December 1597
| Edmund Cecil, 4th Duke of Pembroke
| House of Tudor (by birth)
House of Cecil (by marriage)
|Edmund, Duke of York
|Madeleine de Bourbon
| 15 January 1582
Yorkshire, England
| 14 May 1613 (aged 31)
London, England
|Henry Vll's Chapel, Westminster Abbey
Charlotte of York (15 January 1582 - 14 May 1613) was the eldest daughter of Edmund, Duke of York and Madeleine de Bourbon. She was one of her grandmother's favorites and used it to her advantage. At age 15, she married the Duke of Pembroke.
Early Life
Charlotte of York was the eldest daughter of the Duke of York and his wife, born during the reign of her grandfather, Edward VI. When she was eight years old, her grandfather died and her grandmother, Queen Isabella retired to Richmond Palace after the coronation of her uncle, Alexander I. Her parents became extremely influential courtiers and so Charlotte, along with her siblings were sent to Richmond Palace to be raised by their grandmother. Here, she was brought up alongside her cousins, seeing her parents very little until she joined court after her marriage. During her early years, she formed close ties with her cousins Renata of Glocester, Renee of Clarence, and Henrietta of Kent. The four girls were tutored by John Whitgift from 1590 until they reached marriageable age.
As the eldest daughter of the Duke of York, her hand was highly sought after by the nobility of England. Also, because her hand was a matter of state, considering that she was eleventh in line for the throne, her marriage was arranged by her uncle, the king. She was betrothed to William Cecil, from the extremely loyal Cecil family at the age of thirteen. They married at the end of 1597, advancing the Cecil family and keeping the royal blood loyal to the crown. She was a prominent courtier after her marriage, often at the center of the more literary circles of her day. Her cousins, Renee and Henrietta were often with her, keeping the salons of court well informed on court politics.


text: The Now You See Me! Now You Die! wiki last edited by pikahyper on 05/13/15 05:07AM View full history Illusions twist and bend into reality, as a still-recovering Spider-Man is victim to Mysterio's torture. Will the end of Spider-Man be a rapidly filling swimming pool? When it comes to Mysterio, all bets are off the table!OverviewNow You See Me! Now You Die!Mysterio has chained Spider-Man to the bottom of the Nursing Home swimming pool, and in an instant, Spidey is deluged with water. Using all his energies, he breaks the chains binding him to the pool, and escapes.After the battles between Mysterio and the Kingpin, Pete needs a rest, and swings home. After a good night sleep, as well as a visit from friends, he finds his broken arm completely healed, and feels renewed with energy! Time to tackle the problem again!Peter heads back to the old Parker house, the subject of everyone's interest, when Mysterio reappears, and once again engages Spider-Man in battle. The fight continues to the Nursing Home, where Mysterio announces that he is giving up the search. The treasure, if there ever was one, is long gone, and he opts to quit the battle while he is still ahead. He draws out a tranquilizer gun and shoots Spider- Man in the chest.As Mysterio leaves, he laughs at Peter's attempts to detain him. Quickly batting away his hands, Mysterio declares:"Don't you realize the basic truth? Spider-Man is DEAD!"Notes:Features the Hostess Twinkies Cakes ad, Captain Marvel VS. Professor Sneer.Mark Gruenwald was assistant editor on this issue.


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It wasn't hard to pick out Philadelphia, MS defensive lineman Josh Boyd in DL/OL one-on-ones during Michigan's camp last week. He was the youngster in the red helmet that blew by opponents with both quickness and power. The relative ease with which he was operating left the Michigan coaches continuously searching for stiffer challenges.
"They kept trying to put me on their best offensive linemen," Boyd recalled. "I think I did pretty good during the time I was out there."
Few opponents (with the exception of Chris Lombard) were able to slow Boyd down. He excelled both inside and outside, leaving open the question, which spot does he prefer?
"I would like to play defensive end," he admitted, "but it doesn't matter to me. I'll play defensive tackle if they want me to."
Figuring out where to line this talented prospect up is a problem most coaches would love to have. That certainly appears to be true for the staff in Maize & Blue. They rolled out the red carpet for the Magnolia State star in hopes of letting him know just how much they wanted him in Ann Arbor.
"They showed me around the school and stuff," Boyd reported. "They told me they had a really good academic system up there and I really liked that. I spent time with Coach Rodriguez and he was a real nice guy. He talked to me a little bit told me about everything. He broke a few things down to me about the school. There are a lot of things to do around there. It was pretty good. I spent a lot of time with Coach Shafer. He was a pretty nice and pretty cool guy too. I really got to know him a little bit. Plus I like Coach Barwis, man. He is real cool. He works them hard, but he knows what he is doing I like him a lot."
"The one recruiting me is Coach Hopson, and he is a real nice down to earth person," Boyd continued. "He is the one that gave me my first scholarship when he was at Southern Miss. I already knew him. He doesn't come at you too hard. He just tells you the good things about Michigan. He breaks things down to you and gives you a good understanding about everything. He is real nice."
The treatment Boyd received cat Michigan compared favorably to that which he experienced on a prior camp trip to Mississippi State. Because of that, the Wolverines have made a move in his recruitment.
"I went to Mississippi State, but that was a one day thing and I really didn't do anything there except hang out with the coaches," he said. "The Mississippi State coaches have shown me some good times. I've kind of vibed with those guys too. I really like it up there. When I went to Michigan I did the workout and had a lot of fun. (The distance from home) wasn't bad at all. I really didn't even think about it. I just came up there and had fun. (The time in Ann Arbor) moved them up because I got to see a lot of things that I liked."
How far up remains to be seen. For the time being Boyd is staying mum about his list of favorites.
"I'll have a top list of schools around the time school starts," he said. "I think I'll be taking a few more trips to Florida State, Ole Miss, and LSU. I'm probably going to take some visits in the fall too. I'll probably come back up that way (to Ann Arbor). My decision will come down to the coaches, the players, the atmosphere at the school, and the main thing… the academics."


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Entreprenomics at Wikia
Welcome to the Entreprenomics mini wiki at Scratchpad!
You can use the box below to create new pages for this mini-wiki. Make sure you type
[[Category:Entreprenomics]] on the page before you save it to make it part of the Entreprenomics wiki (preload can be enabled to automate this task, by clicking this link and saving that page. Afterwards, you may need to purge this page, if you still see this message).
- what does it study? (irrelevant) - Not a study: a blog &community portal for the generation &dissemination of ideas in different subjects concerning the social sciences, technology, hands-on entrepreneurship, business &education - all in pursuit of freedom and happiness - Philosophy of it?... - my wiki for the above