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Identifying MicroRNA-mRNA regulatory network in colorectal cancer by a combination of expression profile and bioinformatics analysis
MicroRNAs (miRNAs) are involved in carcinogenesis and tumor progression by regulating post-transcriptional gene expression. However, the miRNA-mRNA regulatory network is far from being fully understood. The objective of this study is to identify the colorectal cancer (CRC) specific miRNAs and their target mRNAs using a multi-step approach.
A multi-step approach combining microarray miRNA and mRNA expression profile and bioinformatics analysis was adopted to identify the CRC specific miRNA-mRNA regulatory network. First, 32 differentially expressed miRNAs and 2916 mRNAs from CRC samples and their corresponding normal epithelial tissues were identified by miRNA and mRNA microarray, respectively. Secondly, 22 dysregulated miRNAs and their 58 target mRNAs (72 miRNA-mRNA pairs) were identified by a combination of Pearson's correlation analysis and prediction by databases TargetScan and miRanda. Bioinformatics analysis revealed that these miRNA-mRNAs pairs were involved in Wnt signaling pathway. Additionally, 6 up-regulated miRNAs (mir-21, mir-223, mir-224, mir-29a, mir-29b, and mir-27a) and 4 down-regulated predicted target mRNAs (SFRP1, SFRP2, RNF138, and KLF4) were selected to validate the expression level and their anti-correlationship in an extended cohort of CRC patients by qRT-PCR. Except for mir-27a, the differential expression and their anti-correlationship were proven. Finally, a transfection assay was performed to validate a regulatory relationship between mir-29a and KLF4 at both RNA and protein levels.
Seventy-two miRNA-mRNA pairs combined by 22 dysregulated miRNAs and their 58 target mRNAs identified by the multi-step approach appear to be involved in CRC tumorigenesis. The results in our study were worthwhile to further investigation via a functional study to fully understand the underlying regulatory mechanisms of miRNA in CRC.
MicroRNAs (miRNAs) are one family of small (~22 nucleotides), non-coding RNAs. They regulate gene expression post-transcriptionally through binding to the complementary sites of target mRNAs in the 3'UTR
Many aberrant expressed miRNAs were found in CRC tissues by miRNA microarray assay
MiRNAs regulate target gene expression in two ways, mRNA degradation or translation inhibition
In this study, we applied the multi-step approach by constructing the aberrant paired miRNA-mRNA expression profiles and the clusters of miRNA target genes to investigate the role of miRNA in CRC.
As shown in Figure
Figure 1
A multi-step approach was adopted to identify the mRNA targets of CRC-specific miRNAs in colorectal cancer
Step 1, both miRNA and mRNA microarray tests for identifying significantly dysregulated miRNAs and mRNAs in colorectal cancer. Step 2, Pearson correlation analysis for anti-correlationship of dysregulated miRNAs and mRNAs. Step 3, TargetScan and Mirnada predicting the target-relationship miRNA-mRNA pairs.
Further in detailed explanation, the target genes of dysregulated miRNA in CRC tissues should meet the following requirements: 1)the microRNAs and mRNAs were differentially expressed between CRC tissues and corresponding normal samples 2) the expression level of miRNAs and mRNAs were significantly anti-correlated (P < 0.05) 3) the mRNA is a putative target of miRNA predicted by both TargetScan
miRNA and mRNA aberrant expression profiles in CRC samples
The miRNA expression profiles in 8 CRC tissues and their corresponding adjacent normal tissues were determined using miRNA microarray analysis. Histologically, the 8 CRC cases were all moderately differentiated, non-mucinous adeno-carcinoma, micro-satellite stable. The clinical data of the 8 CRC cases are listed in Additional file
Additional file 1
Supplemental tables and figures
Click here for file
Figure 2
Heatmap of 32 differentially expressed miRNA in colorectal caner samples compared to paired adjacent normal tissue
FDR < 0.05 for students t-test and q-value < 0.05 for SAM were considered as significantly differentially expressed. The data were z-score transformed and were indicated by the color bar below the heatmap.
The mRNA expression profiles of the same 8 paired-samples in miRNA expression profiling were analyzed by Whole Human Genome Agilent 4 × 44 K Oligonucleotide Microarray (Agilent). After between-array normalization performed by Linear Models for Microarray Data (R-package Limma)
Additional file 2
Differentially expressed mRNA in CRC tissues
Click here for file
The microarray data was deposited to the NCBI Gene Expression Omnibus (accession GSE35982)
Identifying target genes by anti-correlation and target prediction
Pearson's correlation analysis was applied to the 32 significantly differentially expressed miRNAs and significantly differentially expressed 2916 mRNAs. Since miRNAs act as negative regulators, up-regulated miRNAs resulted in down-regulated target mRNAs, and vice versa. A total of 17360 miRNA-mRNA pairs (Additional file
Additional file 3
Significantly anti-correlated miRNA-mRNA pairs
Click here for file
Additional file 4
Target mRNAs of up-regulated miRNAs
Click here for file
Additional file 5
Target mRNAs of down-regulated miRNAs
Click here for file
Pathway analysis of the miRNA targets
To obtain a better understanding of biological function of miRNAs dysregulated in CRC, function analysis of the miRNA target genes was carried out by Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.7
Additional file 6
Significantly enriched GO biological processes
Click here for file
Validation of the expression level of miRNA and mRNA in an extended cohort of CRC tissues by qRT-PCR
To validate the reliability of the microarray test,6 up-regulated miRNAs (mir-21, mir223, mir224, mir29a, mir29b, and mir27a) and their 4 down-regulated target mRNAs (SFRP1, SFRP2, RNF138, and KLF4) were selected for qRT-PCR examination in 40 matched CRC/normal mucosa tissues whose clinical data were listed in Additional file
As demonstrated in Figure
Figure 3
Validation of differentially expressed miRNA and target mRNA by qRT-PCR (n = 40)
A. The expression levels of 5 miRNAs were significantly up-regulated. B. The expression levels of 4 mRNA targets in CRC samples and adjacent normal tissues. The expression levels were expressed as –ΔΔCT after normalized with beta-actin.
Additionally, the Pearson's correlation coefficient between the expression levels of miRNA-mRNA pairs were calculated, and two identified miRNA-mRNA pairs, mir-224 and SFRP2, mir-29a and KLF4 were found significantly anti-correlated in qRT-PCR tests. However, 2 miRNA-mRNA pairs (mir-29a and SFRP1; mir-21 and SFRP1) not predicted by the two exist between miRNAs and SFRP1 (Table
In vitro analysis revealed expression of KLF4 is regulated by mir-29a
To further validate identified miRNA-mRNA interactions, the transfection assay was carried out to demonstrate the relationship between mir-29a and its identified target, KLF4. HCT-116 cells were transfected with mir-29a mimics and mir-29a inhibitor. The qRT-PCR and Western-blot revealed that the KLF4 mRNA and protein expression were significantly down-regulated by the mir-29a mimics (Figure
Figure 4
Validation of relationship between KLF4 and mir-29a through in-vitro assay
A. The transfection of mir-29a mimics into HCT-116 cells down-regulates KLF4 mRNA expression (Left, QRT-PCR) and protein expression (Right, Western blot). B. The transfection of mir-29a inhibitor into HCT-116 cells up-regulates KLF4 mRNA expression (Left, QRT-PCR) and protein expression (Right, Western blot). The experiments were independently repeated three times and GADPH served as control. ***, P < 0.001.
miRNAs, acting as negative regulators of gene expression, regulate various biological processes via inhibiting the expression of their target genes. Increasing evidence shows that some miRNAs play critical roles in tumorigenesis of CRC and have a potential clinical value in diagnosis, treatment and prognosis evaluation for CRC
In this study, a multi-step approach to identify the mRNA targets of dysregulated miRNAs in CRC was adopted. Firstly, in the same cohort of samples containing CRC tissues and their corresponding adjacent tissues, the expression profiles of miRNAs and mRNAs were screened. Secondly, all possible pairs of miRNA-mRNA with an anti-correlationship were identified by Pearson's correlation analysis, implying that these pairs of miRNA-mRNA may contain both the direct target-relationship and indirect anti-correlationship pairs. Thirdly, the reliability of the identified target-relationship pairs of miRNAs-mRNAs was assessed and confirmed by using both TargetScan and miRanda databases. Finally, the differential expression and their anti-correlationship of 6 miRNAs and 4 mRNAs were validated by qRT-PCR and the regulatory effect of mir-29a on the expression of target of KLF4 mRNA was validated by in-vitro analysis. Through the multi-step approach, 72 miRNA-mRNA pairs containing 22 dysregulated miRNAs and their 58 target mRNAs in CRC were identified, and the Wnt signaling pathway was the only affected pathway based on pathway analysis for the miRNA targets.
Compared with previous studies on the miRNA expression profile of CRC, including miRNA microarray
In this study, an important note should be added that a very strict criterion was adopted to identify the miRNA targets. Briefly, the target mRNA should be differentially expressed, significantly anti-correlated with its miRNA regulator, and predicted by two databases, TargetScan and miRanda. Based on this strict criterion,our final identified miRNA-mRNA pairs have a high probability of involvement in CRC tumorigenesis. The following analysis will support this high probability. On one hand, comparing the mRNA targets (n = 4338) of the dysregualted miRNAs (n = 32) predicted just by TargetScan, only a small portion of the target genes (244/4338, 5.6 %) were significantly differentially expressed in CRC, and were selected for further analysis, while a great number of mRNAs (4094/4338, 94.4 %) were not differentially expressed in CRC tissue, and were not selected for further analysis. The fact that these 4094 genes were predicted as miRNAs targets but not significantly differentially expressed, suggested that the expression of these 4094 mRNAs might not be regulated by the 32 dysregulated miRNAs, but by other mechanisms such as regulation by other miRNA, methylation, cis-regulatory elements, etc. which may act and counteract the regulation of miRNA. On the other hand, Pearson's correlation analysis selected 17360 miRNA-mRNA pairs whose expression levels were significantly inversely correlated, however only 72 target relationships of miRNA were finally identified. The existence of the rest of 17288 miRNA-mRNA pairs may be caused by the indirect regulation.
Another interesting finding was that the expression values of mir-29a showed a narrower range of change than those of its predicted target, KLF4. To rationalize this finding, it is hypothesized that the KLF4 mRNA expression was regulated by many other factors including other miRNAs. Besides mir-29a, KLF4 was also identified as a target gene of mir-7, an up-regulated miRNA with high fold-change. The unparalleled change of miRNA and its target mRNA expression levels could be caused by the two miRNA (mir-29a and mir-7) targeting one gene (KLF4). Inspired by this finding, the relationship between fold-change of mRNAs expression and the number of corresponding miRNA regulators were further analyzed. In the identified 72 miRNA-mRNA pairs, three groups can be classified: 1) 2 mRNAs were regulated by 3 miRNAs, 2)10 mRNAs were regulated by 2 miRNAs, and 3) the rest (60 mRNAs) were regulated by 1 miRNA. However, the fold-changes of the gene mRNA expression between these three groups do not reach the statistical significance, indicating the gene expression regulation is very complex and dynamic with impact from multi-factors.
miRNAs regulate diverse biological processes via regulating the expression of target genes. In this study, only the Wnt signaling pathway, which had been proved to be aberrantly activated in CRC
Nevertheless, the orchestration of these regulatory pairs of miRNA-mRNA differed in different diseases, presenting the disease-specific expression profiles and composing a complex regulatory network. Although many methods had been used to identify the regulatory pairs of miRNA-mRNA in diseases such as microarray and software-prediction, no method possessed the capacity to determine all miRNA-mRNA regulatory pairs. Moreover, since the anti-correlationship of the miRNAs-mRNA expression levels was used as the screening criteria, only those target genes regulated through mRNA degradation could be identified here, target genes regulated by miRNAs through translation inhibition would be lost.
In summary, these new findings of pairs of miRNA-mRNA provide some hints for the mechanisms of CRC tumorigenesis and were worthwhile for further functional analysis. The more complex regulatory networks call for more research in the future.
Based on this research, seventy-two miRNA-mRNA pairs combined by 22 dysregulated miRNAs and their 58 target mRNAs identified by the multi-step approach appear to be involved in CRC tumorigenesis. The identified miRNA-mRNA pairs are worthwhile for further functional studies.
Patients and clinical samples
Forty CRC tissues and matched adjacent normal colorectal mucosa tissues (at least 5 cm from the cancer) were obtained from patients who underwent surgical excision for CRC at Xinhua Hospital, School of Medicine, Shanghai Jiaotong University. The samples were collected according to the following histological prerequisites: the CRC tissues had more than 80 % tumor cells; the matched adjacent normal colorectal mucosa tissues had normal mucosal structure without dysplastic cells. The samples were snap frozen in liquid nitrogen in 5 minutes after surgical resection and were then stored at −80°C temperature until RNA extraction. Informed consents were obtained from all the patients and the study was approved by hospital ethics committee.
RNA extraction
Total RNA was extracted and isolated using mirVana TM RNA Isolation Kit (Applied Biosystem p/n AM1556) following the manufacturer's instructions. The concentration of RNA was measured by NanoDrop spectrophotometer. Total RNA concentration ranged form 100 ng/ul to 1ug/ul. The RNA quality was evaluated by Agilent 2100 Bioanalyzer; RNAs with RIN ≥ 7.0 and 28 S/18 S > 0.7 were accepted for microarray analysis.
MicroRNA microarray procedure
8 colorectal cancer tissues and their corresponding adjacent normal tissues ere selected for microRNA microarray analysis from the 40 matched tissues pairs of CRC tissues and their adjacent normal colorectal mucosa tissues. These 8 pairs of samples shared similar clinical and pathological features that they were all the moderately differentiated, non-mucinous adeno-carcinoma and micro-satellite stable. The total RNA was analyzed by microarray (Agilent V12.0) according to the manufacture's protocol. In brief, 100 ng of total RNA was labeled with Cy3, and then hybridized on a miRNA array (8*15 K,Agilent V12.0) using Agilent's miRNA Complete Labeling and Hyb Kit (p/n 5190–0456). Microarray after hybridization was washed by GE Wash Buffer 1 and GE Wash Buffer 2 (Gene Expression Wash Buffer kit, Agilent p/n 5188–5327). The slides were then scanned in microarray scanner by Agilent scan control software Version A7.0. The data collection, background subtraction and with-in array normalization were carried out by Agilent Feature Extraction (FE) software version 9.5.3. After Between-array normalization and signal filtration (IsGeneDetected, WellAboveNeg) performed by RMA algorithm
mRNA microarray procedure
An amount of 2 ug of total RNA isolated from CRC tissues and corresponding normal tissues were reverse-transcribed into cDNA, and labeled with Cy3 or Cy5. The labeled cDNA was then hybridized on a Whole Human Genome Agilent 4X44K Oligonucleotide Microarray (Agilent) according to the manufacture's protocol using miRNA Complete Labeling and Hyb Kit. The chips were washed using Agilent Gene Expression Wash Buffer Kit (Agilent 5188–5327). Scanning of the microarrays was carried out immediately using the Agilent G2565BA Microarray Scanner System. Images were auto gridded, analyzed and data extracted using Agilent Feature Extraction software (version 9.5.3). Raw expression data generated by the Feature Extraction software was imported into R using the LIMMA package in Bioconductor
Similar to the criteria of identifying dysregulated miRNAs, mRNAs with FDR <0.05 for student's t-test and q-value < 0.05 for SAM were identified as differentially expressed genes.
Target predicting
TargetScanHuman v. 5.1
Pathway enrichment analysis
The pathway enrichment analysis was performed using online database. The Database for Annotation, Visualization and Integrated Discovery (DAVID) v 6.7
Both mRNA and miRNA expression level were detected in 40 CRC tissues and their corresponding adjacent normal tissues by qRT-PCR method using the ABI Detection Kit. For mRNA expression detection, 2 ug of total RNA was reverse transcribed by MMLV transcription according to manufacture's protocol. The cDNA was analyzed by TaqMan assays. qRT-PCR was performed by ONE-STEP PLUS system (ABI). The primers and probes of qRT-PCR were listed in Additional file
The expression level of each mRNA and miRNA was calculated by threshold cycle (CT). The relative expression level was calculated using –ΔΔCT. A median expression sample among all samples was chosen as a calibrator, and U6 as endogenous control for miRNAs expression, beta-actin for mRNAs expression.
Cell culture
Human colorectal cell line HCT 116 was purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. The cell line was incubated in Dulbecco's modified Eagle's medium (DMEM, Invitrogen, Carlsbad, CA, USA) with 10 % FBS.
The hsa-mir-29a mimics, mimics control, hsa-mir-29a inhibitor (anti–miR-95, chemically modified antisense oligonucleotides designed to target specifically against mature miR-29a), miRNA inhibitor control were designed and synthesized from Ribobio (Guangzhou, China). The 4 × 106 of HCT 116 cell were grown in 6-well plates for 24 hours before transfection. The transfection was performed by Roche X-tremeGENE DNA Transfection Reagents according to manufacturer's protocol. Every experiment was performed in triplicates in 6-well cell culture plates with the appropriate controls. Cells were harvested 48 h after transfection.
Western blot
Total proteins from cultured cells were extracted by RIPA Lysis Buffer (Beyotine, Shanghai, China). 40 ug of proteins were electrophoresed through 10 % SDS polyacrylamide gels and were transferred to a PVDF membranes (Millipore) The membranes were incubated with anti-KLF4 Rabbit polyclonal antibody (ab26648,1:1000, abcam), anti-GADPH antibody was chosen as an endogenous control. Membranes were probed with the appropriate secondary antibodies linked to horseradish peroxidase. Antibody binding was visualized using ECL.
Statistical analysis
Statistical analysis was performed using SPSS15.0. Values were expressed as mean ± standard deviation for parametric data. Difference between groups was compared using student's
Authors' contributions
JF, JG and LC designed the study and authored the manuscript. PD, JL and WC collected the samples. GW and WT performed the qRT-PCR analysis. WT carried out transfection assays. PD, JF and JG carried out the microarray test and the bioinformatics analysis. LC, PD, JG and JF analyzed data. All authors read and approved the final manuscript. JF and WT contributed equally to this work.
The project was supported by the Major Program for the Fundamental Research of the Shanghai Committee of Science and Technology (Grant No.10DJ1400504), the Natural Science Foundation of Shanghai (Grant No. 11ZR1424200) and the Key Program for the Fundamental Research of the Shanghai Committee of Science and Technology (Grant No. 09JC1410900). The support on transfection assay from Professor Jiuhong Kang of laboratory of stem cell and epigenetic research, School of Life Science and Technology, Tongji University, Shanghai is appreciated. Thanks for the technical support of microarray from Oebiotech Company and qRT-PCR test from Biotecan Company
MicroRNA biogenesis and cancer
Oncomirs - microRNAs with a role in cancer
MicroRNAs as biomarkers in colorectal cancer
Prognostic Values of microRNAs in Colorectal Cancer
MicroRNAs in colorectal cancer: translation of molecular biology into clinical application
Diagnostic microRNA markers for screening sporadic human colon cancer and active ulcerative colitis in stool and tissue
The impact of microRNAs on colorectal cancer
Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer
Down-regulation of fecal miR-143 and miR-145 as potential markers for colorectal cancer
Over- and under-expressed microRNAs in human colorectal cancer
Initial study of microRNA expression profiles of colonic cancer without lymph node metastasis
MicroRNA profiling differentiates colorectal cancer according to KRAS status
Role of miR-143 targeting KRAS in colorectal tumorigenesis
Overlapping expression of microRNAs in human embryonic colon and colorectal cancer
Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening
MicroRNA-95 promotes cell proliferation and targets sorting Nexin 1 in human colorectal carcinoma
MicroRNA-21 and PDCD4 expression in colorectal cancer
MicroRNA-221 inhibits CDKN1C/p57 expression in human colorectal carcinoma
MicroRNA-342 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Directly Targeting DNA Methyltransferase 1
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets
Prediction of microRNA targets
Human MicroRNA targets
The microRNA.org resource: targets and expression
MicroRNAs: genomics, biogenesis, mechanism, and function
How do microRNAs regulate gene expression?
Computational identification of hepatitis C virus associated microRNA-mRNA regulatory modules in human livers
Identifying mRNA targets of microRNA dysregulated in cancer: with application to clear cell Renal Cell Carcinoma
Processing of Agilent microRNA array data
Significance analysis of microarrays applied to the ionizing radiation response
Normalization of cDNA microarray data
The Pathobiology of Kruppel-like Factors in Colorectal Cancer
The Wnt antagonist sFRP1 in colorectal tumorigenesis
Hypermethylation and expression regulation of secreted frizzled-related protein genes in colorectal tumor
NARF, an nemo-like kinase (NLK)-associated ring finger protein regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor (TCF/LEF)
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
The Wnt antagonist sFRP1 is downregulated in premalignant large bowel adenomas
SFRP4 was overexpressed in colorectal carcinoma
Wnt/beta-catenin signaling: components, mechanisms, and diseases
Sjoblom T, Barad O, Bentwich Z, Szafranska AE, Labourier E et al: The colorectal microRNAome
Downregulation of miR-195 correlates with lymph node metastasis and poor prognosis in colorectal cancer
EGFR promotes lung tumorigenesis by activating miR-7 through a Ras/ERK/Myc pathway that targets the Ets2 transcriptional repressor ERF
Four miRNAs associated with aggressiveness of lymph node-negative, estrogen receptor-positive human breast cancer
microRNA-7 inhibits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma
Regulation of epidermal growth factor receptor signaling in human cancer cells by microRNA-7
Noncanonical Wnt signaling in vertebrate development, stem cells, and diseases
Wnt signaling and cancer


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London, July 23: Duchess of Cambridge Kate gave birth to a baby boy on Monday afternoon at a hospital here. The birth was announced via a formal press release issued by the Kensington Palace, which said that the royal son was born at 4.24 pm, local time.
The Duke of Cambridge was present at the grand occasion, the palace statement said. The Queen, The Duke of Edinburgh, The Duchess of Cornwall, Prince Harry, The Prince of Wales and members of both families were delighted with the news.
Prince William was with the duchess throughout the labour. An overjoyed Prince Charles and his wife Camilla, Duchess of Cornwall, said grandparenthood is a unique moment in one's life and they were extremely proud and happy.
British Prime Minister David Cameron, US President Barack Obama and First Lady Michelle Obama and Australian Prime Minister Kevin Rudd congratulated the royal family on the happy occasion.
OneIndia News

subset: commoncrawl


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What does cynically mean?
Definitions for cynically
Here are all the possible meanings and translations of the word cynically.
Princeton's WordNet
with cynicism; in a cynical manner
"Larsen's frost-blackened lips curved cynically"
in a cynical manner
Webster Dictionary
in a cynical manner
Chaldean Numerology
The numerical value of cynically in Chaldean Numerology is: 3
Pythagorean Numerology
The numerical value of cynically in Pythagorean Numerology is: 5
Sample Sentences & Example Usage
I once said cynically of a politician, Hell double-cross that bridge when he comes to it.
I once said cynically of a politician, 'He'll doublecross that bridge when he comes to it.'
I was wary of making images that fed into this idea of the heroic strong women fighters, i think that the female fighters are used quite cynically by Kurdish leaders (who) know very well how attractive this image is to the Western media.
We might make a public moan in the newspapers about the decay of conscience, but in private conversation, no matter what crimes a man may have committed or how cynically he may have debased his talent or his friends, variations on the answer Yes, but I did it for the money, satisfy all but the most tiresome objections.
Images & Illustrations of cynically
Translations for cynically
From our Multilingual Translation Dictionary
- cínicamentCatalan, Valencian
- κυνικάGreek
- cínicamenteSpanish
- kyynisestiFinnish
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"cynically." Definitions.net. STANDS4 LLC, 2017. Web. 23 Jul 2017. <http://www.definitions.net/definition/cynically>.


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Promoting my first set! Check it out!! http://www.zivity.com/models/goddessoftheocean/photosets/1


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Author: ABel [TeamX] Last revision: 16 Jan 2001
This document is an attempt to describe the structure of ZAR images (image files in "Fallout Tactics: Brotherhood of Steel" game).
The ZAR format is the property of Interplay as stated in the "Fallout Tactics" readme file.
Note: this document introduces some terms, which are quoted through the document. The author does not pretend these terms are accurate or adequate.
One more note: the purpose of several fields is unknown, so some suppositions on their meaning are made, but neither can they be proved nor rejected by the author at the time of writing the document. Such hypotheses are marked with '(???)'.
I. Main featuresEdit
1. The zar-image can be "stand-alone" and "embedded". I.e., it can be a single file (*.zar) or a part of some larger file (included into a Tile (*.til) or a Sprite (*.spr)). Class of "Embedded" ZARs can be subdivided into "complete" or "incomplete".
"Complete" ZAR contains all the information necessary for its displaying, though the enclosing file can provide some additional fields changing the way the image is showed.
"Incomplete" images do not have they own palettes (such ZARs can be observed in the Sprites). The color information is taken from the enclosing entity's palette.
2. The format uses the palette to store the color information. The palette entries are RGBA quadruples, besides the usual 256-level RGB there is an alpha- (or transparency-) channel, which is used to display shadows or anti-aliased images against any background. The alpha is also 256-leveled, where 255 stands for full opacity and 0 means full transparency.
3. The format uses a kind of RLE compression (though the term RLE is not quite adequate here). Pixel information is stored in blocks consiting of a header and pixel data bytes (optionally).
II. Format specificationEdit
This specification uses C-like constructions and expressions along with two self-explanatory pseudo-keywords: 'optional' and 'variable_length'. The following types are used:
CHAR one byte integer (unsigned char in C) DWORD four bytes integer (unsigned long in C). ZAR_IMAGE ::= variable_length struct { ZAR_HEADER image_header; optional ZAR_PALETTE palette; DWORD data_length; variable_length union { CHAR image_data[data_length]; RLE_BLOCK blocks[]; } }
ZAR_HEADER ::= struct { CHAR signature[6]; // zar-image signature, must be '<zar>\0' CHAR zar_type; // must be 0x33 or 0x34 CHAR dummy1; // nothing (???) DWORD image_width, image_heigth; CHAR palette_present_flag; }
If palette_present_flag is 0x01 then zar_image includes a palette.
ZAR_PALETTE ::= variable_length struct { DWORD color_count; // count of color entries in the palette; // less than or equals to 256 (???) PAL_ENTRY colors[color_count]; // color entries optional CHAR default_color; // index of default (or background) // color (???); the field is // included only when the zar_type // is 0x34. } PAL_ENTRY ::= struct { CHAR blue, green, red, alpha; } RLE_BLOCK ::= variable_length struct { CHAR rle_command : 2; // command (two bits) CHAR block_length : 6; // data length (six bits) CHAR pixel_data[]; // pixel information; the length of this // item can be zero, block_length or // twice the block_length, depending on // the value of rle_command. }
III. Decompression schemeEdit
Pixel data is RLEncoded in the blocks of type RLE_BLOCK (or in the image_data array - they are the same things). The decompression starts from the top left corner of the image and stops when all the image_data array is processed or when the all the image_height scan lines are rendered, whichever happens first. The processing is carried out from left to right in the horizontal direction. On reaching of the right border (image_width field) the processing is switched to the start of the next scanline of the image.
Every block determines the color of the block_length consecutive pixels. The following table describes the treatment of pixel_data array and the color values of the next block_length image pixels depending on the rle_command used in a block.
rle_command | pixel_data | image pixels -------------+----------------------+--------------------------------------- 0 | no data at all; | next block_length pixels are filled | zero length | with 00:00:00:00 (R:G:B:A) color, or | | simply skipped -------------+----------------------+--------------------------------------- 1 | CHAR color_inds[b_l] | pixel color (RGB) is taken from the | | respective palette entries, the | | alpha-value (A) is set to 0xFF -------------+----------------------+--------------------------------------- 2 | { CHAR color_ind; | pixel's RGBA is specified by the pair | CHAR alpha_val;} | from pixel_data array | pairs[b_l] | -------------+----------------------+--------------------------------------- 3 | CHAR alpha_vals[b_l] | pixel's color (RGB) is set to the RGB | | value from colors[0] or may be from | | colors[default_color] (???), | | the alpha value is taken from the | | pixel_data array
(???) The block cannot span multiple scanlines. If the block defines more pixels than can fit into the rest of current scanline, the redundant data is to be ignored. (???)
If you have any questions regarding this document, or have found errors, mistakes or inaccuracies, please do not hesitate to email me to firstname.lastname@example.com or email@example.com
Found in TeamX's offline docs


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View the 5 routes on this map:
Ride #1 - Poudre Canyon & North Park
Ride #2 - Rocky Mountain National Park
Ride #3 - Short Sweet and Sassy
Ride #4 - Riding High
Ride #5 - America the Beautiful
For more national routes, visit:
Scenic Road Maps
Denver & Beyond
When renting a motorcycle at Mile High Rentals, think about purchasing our City Escapes Maps that feature scenic rides in the Colorado region and Sturgis.
These water and tear resistant maps feature detailed, step by step directions to help you easily navigate scenic rides in the Denver, Colorado Area as well as the Southwest and Sturgis.
Motorcycle Rides: Customer Case: $29.95
This wallet case Includes 3 maps:
• Denver Map / Traveler size: City Escapes
• Large Southwest, 4 Corners: AZ, NM, CO & UT.
• Rally Rides Map of Sturgis
Denver Area Scenic Rides Map:
This City Escapes Map features 5 Scenic Rides that are color-coded on the map, suggested stops, a list of Places to Visit, and more. Makes the perfect gift for a fellow rider.
Stop by today and pick up a map and plan your next road trip!
Check out our hotel recommendations:
Denver Lodging
We've had the pleasure to welcome several tour groups, check out their trip photos: Black Hills Ride, West Forever, JD'S Motorsickle Tour. View more rental group photos on our Facebook page.
Reservations | Rental Models | Contact
Us | Map | Events | Mile
High Harley | Testimonials | Requirements
Mile High Rentals All rights reserved Website Design by IJDesign
Shop our online store, Planetharley.com for the latest gear:
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This exciting vacation introduces you to the rich history, scenic wonders, and cultural treasures of China and Tibet. Start in exciting Hong Kong. Take a cable-car ride up Victoria Peak for panoramic views and a visit of Aberdeen fishing village for a sampan ride on the stunning harbor. Free time on your own gives you a chance to explore the many markets, taste some authentic Chinese food, listen to the sounds of the city, or take an optional excursion. In Shanghai, walk along the Bund, see stunning displays of ancient bronze and jade at the Shanghai Museum, and enjoy a traditional dim-sum feast. You will also attend a high-flying acrobat show and take a fascinating walking tour through the former French Concession where tree-lined streets and Art Deco architecture transport you to a bygone era. Then, fly to Yichang and begin your Yangtze River cruise with a private welcome reception on board the Century Legend. Shore excursions include a walking tour of a riverside community to see its beautiful pagoda, a tour of the Three Gorges Dam site, and a small boat cruise on the emerald-green waters of Shennv Stream. Travel to Lhasa, Tibet's mystical capital, for visits to a local family home for lunch, a local bazaar, and the imposing Potala Palace, former winter home of the Dalai Lama. You will also dine in a local restaurant and be entertained with traditional Tibetan music and dance. In Xi'an, see the famed Terracotta Warriors up close from a special VIP viewing area and learn how to write ancient Chinese calligraphy. Lastly, in Beijing, visit the Forbidden City with its Imperial Palace, sample authentic Peking Duck, and walk along the incomparable Great Wall of China. You will also explore one of the city's ancient alleys by rickshaw and experience a cultural performance at the Peking Opera or the Legend of Kung Fu show.
City Highlights
Cruise: deluxe 4-night cruise from Yichang to Chongqing in a Deluxe Stateroom with two lower beds, private facilities, and balconyHong Kong: cable-car ride up Victoria Peak; sampan ride on harbor; visit Stanley Market; Host service during your stayShanghai: sightseeing including headsets; visit the Bund, Yu Yuan Gardens, a silk factory, and the Shanghai Museum; dim sum lunch; walking tour of the French Concession; see an acrobatic showYichang: begin your Yangtze cruise aboard the Century Legend; private welcome receptionThree Gorges Dam Site: sightseeing of the impressive dam siteShennv Stream: boat excursion down one of the Yangtze's lesser gorgesShibaozhai: walking tourChongqing: visit a local zoo to see pandasLhasa: visit the Summer Palace, a Tibetan's home including lunch, a Tibetan monastery, a Tibetan hospital, Potala Palace, Jokhang Temple, and a local bazaar; cultural show with traditional Tibetan music and danceXi'an: calligraphy class; sightseeing including headsets; visit the Terracotta Warriors including the VIP viewing area and Dayan Pagoda; noodle-making demonstration; "Tang Dynasty" dinner showBeijing: hutong tour by rickshaw; sightseeing including headsets; visit the Temple of Heaven, Forbidden City, Imperial Palace, Tian'anmen Square, Great Wall; Peking Opera performance or Legend of Kung Fu Show; Peking Duck farewell dinner
American breakfast daily10 lunches11 dinners including a welcome dinner in Shanghai and a Peking Duck farewell dinner in Beijing
NOTES: This trip is for travelers who are fit. You will visit regions reaching almost 14,000 ft. in elevation. Accommodations in the Gobi Desert are in traditional Mongolian gers with private bathroom and shower facilities.


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This family-friendly Sydney hotel is located in the entertainment district, within a 5-minute walk of Darling Harbour, IMAX Sydney, and St Andrew's Cathedral. ...
Situated in a national park, this hostel is 2.4 mi (3.9 km) from Ku-ring-gai Chase National Park and within 6 mi (10 km) of Newport Beach and The Basin. Marramarra ...
Nestled on the beach, this Narrabeen hotel is within 6 mi (10 km) of Dee Why Beach, Newport Beach, and Curl Curl Beach. Brookvale Oval and Warringah Mall are ...
Situated on the riverwalk, this caravan park/campground is 0.5 mi (0.9 km) from North Narrabeen Beach and 2.1 mi (3.3 km) from Mona Vale Beach. Newport Beach ...
This Collaroy hotel is on the beach, 2.5 mi (4.1 km) from Dee Why Beach, and within 6 mi (10 km) of Newport Beach and Curl Curl Beach. Brookvale Oval and Warringah ...
This 25-story hotel has pedestrian links to 2 of the city's most popular shopping precincts, the Pitt Street Mall and the Queen Victoria Building.
Situated in Palm Beach, this luxury hotel is within 3 mi (5 km) of Governor Phillip Park, Palm Beach Golf Club, and Avalon Beach. Avalon Golf Course is 2.7 mi ...
Situated near the bay, this motel is within 1 mi (2 km) of Newport Park and Newport Beach. Bungan Beach and Florence Park are also within 2 mi (3 km).
Situated in Mascot, this hotel is 2.7 mi (4.3 km) from Marrickville Metro and within 6 mi (10 km) of University of Sydney and University of New South Wales. ...
Situated in the suburbs, this hotel is within 1 mi (2 km) of Brookvale Oval and Warringah Mall. Dee Why Beach and Curl Curl Beach are also within 2 mi (3 ...
Orbitz makes it easy to find the best Northern Beaches hotel deals for your trip. With an outstanding selection, easy-to-use search tools and descriptive hotel profiles, you'll find Northern Beaches hotel deals in a wink..
Whether you're searching for hotels in Northern Beaches on business, or hunting for a family getaway, Northern Beaches hotel options are only a click away. Find hotels in Northern Beaches with the location, star-rating and facilities you need. Narrow your search results even further by star rating, hotel chain and amenities, such as free parking, babysitting services, or fitness centers. And with area maps, you can view Northern Beaches hotels close you are to the airport and nearby attractions.
Thanks to photos and virtual tours, you can look before you book your Northern Beaches hotel reservation. Check out Northern Beaches hotel properties using interactive tools which allow you view hotel rooms, common areas and key features. On Orbitz, you can also learn more about hotels in Northern Beaches with unbiased reviews from verified Northern Beaches hotel guests. We ask guests to offer candid feedback on services, comfort, location, value and amenities.
Orbitz works around the clock to find exclusive offers and Northern Beaches hotel deals, just for you. Sign up for deals by e-mail where you'll get first crack at money-saving extras like promo codes, resort credits, free nights and more. You can also "Like" Orbitz on Facebook and follow us on Twitter to receive up-to-the-minute news on hotel discounts, giveaways and more.
When it comes to finding hotels in Northern Beaches, an Orbitz specialist can help you find the property right for you. Chat live or call 1-800-454-3743 any time for help booking your hotels in Northern Beaches. Our team of experts can help you pinpoint Northern Beaches hotels options suited to your tastes and budget.
On Orbitz, Northern Beaches hotel deals mean flexibility. If you need to change your Northern Beaches hotel reservations, or cancel your stay, Orbitz doesn't charge cancel or change fees. Orbitz also offers great selection on airline tickets, vacation packages, and car rental deals to so you can plan your trip to Northern Beaches all in one place.
* Prices reflect the lowest "base rate" found over the next 30 days. Rates are subject to change and may not include taxes and fees, hotel service charges, extra person charges, or incidentals, such as room service. All rates are displayed in USD unless otherwise noted. Converted rates are provided for your convenience. They are based on today's exchange rate, but the hotel will charge you in the local currency.


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Credible minimum deterrence(Redirected from Minimum Credible Deterrence)
Credible Minimum Deterrence is the principle on which India's nuclear strategy are based.
It underlines no first use (NFU) with a second strike capability, and falls under minimal deterrence as opposed to mutually assured destruction. India's tentative nuclear doctrine[1][2] was announced on August 17, 1999 by the then secretary of the National Security Advisory Board, Brajesh Mishra.
Later this draft was adopted with some modifications when the Nuclear Command Authority was announced on January 4, 2003. A significant modification was the dilution of the NFU principle to include nuclear retaliation to attacks by biological and chemical weapons.
Indian National Security Advisor Shri Shivshankar Menon further signaled a significant shift from "no first use" to "no first use against non-nuclear weapon states" in a speech on the occasion of Golden Jubilee celebrations of National Defence College in New Delhi on October 21, 2010, a doctrine Menon said reflected India's "strategic culture, with its emphasis on minimal deterrence."[3][4]
See alsoEdit
- ^ Mishra, Brajesh (August 17, 1999). "Draft Report of National Security Advisory Board on Indian Nuclear Doctrine". Archived from the original on January 16, 2000. Retrieved May 15, 2013.
- ^ Hosted at www.pugwash.org - Draft Report of National Security Advisory Board on Indian Nuclear Doctrine
- ^ Speech by NSA Shri Shivshankar Menon at NDC on "The Role of Force in Strategic Affairs": Web-site of Ministry of External Affairs (Govt. of India)
- ^ NSA Shivshankar Menon at NDC (Speech) : india Blooms Archived August 10, 2011, at the Wayback Machine.
|This article about government in India is a stub. You can help Wikipedia by expanding it.


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EASTPORT – Norma Irene Hoffman, 84, wife of the late John E. Hoffman, died Nov. 10, 2005, at a Bangor hospital. She was born Aug. 18, 1921, in Bolster Mills, the daughter of Ivan and Bertha (Matilla) Nicholes. Norma loved living in Maine. She was a member of the Jehovah's Witnesses of Calais. She is survived by a daughter, Dorothy J. Sherman: and a son, John N. Hoffman, both of Greenfield, Mass.; seven grandchildren and three great-grandchildren. She was also survived by two brothers and three sisters. In addition to her parents and her husband she was predeceased by one son, Ronald C. Hoffman. At the request of the family services will be private. A service of Brookings-Smith, 133 Center St., Bangor.
Posted Sept. 25, 2008, at 11:15 p.m.


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Although he initially said he wouldn't do it a third time, Ricky Gervais will be back to host the Golden Globes in January.
The Hollywood Foreign Press Association voted for the controversial British comedian's return after meeting with NBC official Doug Vaughn, reports Variety and ComingSoon.net. The HFPA confirmed it via Twitter.
Tweeted Ricky moments ago:
"Just told Billy Crystal he'd better not use any of my holocaust or pedophile material at The Oscars. He agreed (true)"
After taking heat for some of his jarring jokes, Gervais dismissed the possibility of coming back, but he has warmed to the idea and last month met with HFPA President Aida Takla-O'Reilly in Paris to discuss it, reports ComingSoon. He said recently that the idea of being back on stage "is very tempting."
The Golden Globes awards ceremony will be held Jan. 15 at the Beverly Hilton Hotel. It will air on NBC.
Update: Gervais, 50, posted a YouTube clip on his Twitter page of his opening monologue from the 2011 show, promising "This (times) 10."
And adding, "I'm telling you now If you don't like it... (Expletive) you! If you do like it.. Love you!"
Sign up for Lifeline Live e-mail alerts


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RECIPE SECTION - Over 10,000 Recipes
Home | Food Articles | Food Trivia | Today in Food History | Food Timeline | Recipes
Cooking Tips | Videos | Food Quotes | Who's Who | Trivia Quizzes | Food Poems
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and other Publications
An extensive selection of free food, beverage & agricultural magazines, e-books, etc.
Makes 4 breakfast or dessert servings.
• 8 crepes, use your favorite recipe or store bought
• 3 Tablespoons butter
• 2 cups mixed fresh berries: blueberries, blackberries, raspberries, strawberries
• 2 oranges, zested and sectioned
• 1/ 4 cup sugar
Stack crepes on a plate and cover with foil. Heat in a 300 degree oven for 10 minutes along with 4 serving plates. In a large sauté pan melt butter. Add berries, orange sections, orange zest and sugar. Cover and sauté until the berries are softened and heated through and the sauce thickens, approximately 5 minutes.
To assemble, place crepe on a warm plate. In one corner of a crepe, spoon on 1/4 cup fruit mixture. Fold in 1/2 then again into 1/4. Repeat process serving two per person. Keep prepared crepes warm while assembling.
Serve with vanilla yogurt or whipped cream if desired.
Nutrients per serving:
Calories 361, calories from fat 135, fat 15 gms, saturated fat 8 gms, cholesterol 159 mg, sodium 333 mg, dietary fiber 4 gms, protein 7 gms
Photo Copyright © 2010 Chilean Fresh Fruit Association Media Center
Recipe Courtesty Chilean Fresh Fruit Association
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All contents are copyright © 1990 - 2017 James T. Ehler and www.FoodReference.com unless otherwise noted.
All rights reserved. You may copy and use portions of this website for non-commercial, personal use only.
Any other use of these materials without prior written authorization is not very nice and violates the copyright.
Please take the time to request permission.


text:
Xenotransplantation and Animal Welfare
Olsson, K.
Bibliographic Citation
Transplantation Proceedings 2000 August; 32(5): 1172-1173
MetadataShow full item record
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Validating Animal Models for Preclinical Research: A Scientific and Ethical Discussion Varga, Orsolya E; Hansen, Axel K; Sandøe, Peter; Olsson, I Anna S (2010-06)The use of animals to model humans in biomedical research relies on the notion that basic processes are sufficiently similar across species to allow extrapolation. Animal model validity is discussed in terms of the similarity ...


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- Sold for: Start Free Trial or Sign In to see what it's worth.
- Item Category: Toys, Dolls, Games & Puzzles
- Source: eBay
- Sold Date: Jun 29, 2009
- Channel: Auction House Sell similar item on eBay
"DAIRY FARMERS"!WOODLAND SCENICS!! Highly Detailed. Items are part of a large sale that included TONS of HO and N Scale rolling stock, structures, kits, vehicles and just about everything else you need for Bid with confidence, read my feedback! Do yourself a favor and PLEASE, PLEASE, PLEASE check out my other auctions and my E-BAY store. Great deals for everyone on everything. Shipping is a CHEAP, CHEAP, CHEAP $1.87 and I will combine multiple wins to save you money. E-mail with any questions before you bid. If you don't plan on paying, please don't bid. Thanks for looking and have a great day. The free listing tool. List your items fast and easy and manage your active items.


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A NEW chairman for Wychavon District Council has officially taken over.
At Wednesday's council meeting (May 17) Cllr Frances Smith, handed over her chain to incoming chairman, Cllr. Liz Eyre, the well-known member for Broadway.
The first order of council was to welcome newly elected members Cllr. Matthew Snape for Evesham South and Cllr. Sital Harris for Droitwich South East.
Cllr Eyre said: "Many congratulations to Cllr. Frances Smith on a such a successful year.
"I'm so looking forward to what is ahead and I have a few areas that I'm keen to work on.
"The first is around faith. As someone with a very strong faith I want to ensure we continue to make this a part of this council, however, I want to make sure that we truly reflect our community.
"Therefore I will not be appointing one chaplain – instead I will have representatives from several faiths joining us throughout the year.
"I have a number of themes that I would like to focus on – these are mental health, physical health, heritage and culture."
Cllr. Frances Smith, outgoing Chairman for Wychavon said "I would like to thank everyone who has supported me throughout this most enjoyable year.
"I'm very pleased to have met so many interesting people and to have had this opportunity.
"I'm also thrilled to be making a donation of £3,500 to St. Richard's Hospice and thank you to everyone who has contributed.
"I wish Cllr. Eyre all the best for her coming year and I have no doubt that she will get as much out of it as I have."
The chairman's consort for public engagements will be her husband, Doug Eyre.
Anyone who would like the Chairman to support their event, contact the Wychavon Support Services team on 01386 565201 or email firstname.lastname@example.com.


text:
Oakland, Berkeley, And East Bay News, Events, Restaurants, Music, & Arts
The Lesher Center for the Arts gallery displays work by contemporary and historic artists alike.
Located at the base of the Berkeley hills, the Ames Gallery is the West Coast's only gallery featuring contemporary paintings, drawings, and sculpture created by self-taught, visionary, and outsider art in addition to antique Americana folk art.
This spacious gallery, once an industrial warehouse, now boasts a stage for performances, two sculpted copper and steel bars for mixers, and rotating exhibitions of contemporary art showcasing local and international artists, world class DJs, and film screenings.


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Discover a selection of top forums perfectworld lamb on the internet. Come to share your passion on forums perfectworld lamb and meet other fans of perfectworld lamb. Then, why don't you create your own forum of perfectworld lamb ?
Imagine - Perfect World Raging Tides Guild Forum
imagine, #perfectworld, myst, maplestory, global, ragingtides, faction, guild, forum, maple, bellocan


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(300 m2 / 3,240 SQ. ft - 3 Bedrooms & 2 ½ Baths)
An expansive entry hall that opens to a large living/dining/kitchen space provides a grand welcome to this spacious yet intimate Villa. Outdoor and indoor living space meld together with covered terraces opening up from two sides of the living room. Three bedrooms are located upstairs, including the master suite with a large covered balcony, bathrooms with shower and oversized tub, and walk-in closet. Both the second and third bedrooms enjoy private balconies. Two-car garage and laundry area included. Enclosed backyard can be enhanced with a private swimming pool.
(Click on pictures below to view larger images)


text: There are two "good" places to have sex in a truck stop. The obvious choice is in a truck. This is not private--the movement of the truck's cab is an unmistakable sign that sexual relations are occurring. A better (more discreet) place is in the bathroom. Most truck stops have private bathrooms with showers. Beware that truck stops are rife with unsavory characters like prostitutes, drug dealers, speed freaks and truck drivers (lol). As such, they are also frequented by law enforcement.


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Apatite is a common mineral with a particularly wide range of colours, including colourless (white), pink, yellow, green, blue and violet. In non-gem quality, very large crystals occur which can weigh up to several hundredweight. Gem quality crystals, however, are quite scarce and fairly small. Apatite is found in very small amounts in virtually all igneous rocks, while large crystals sometimes occur in marble.
The name apatite is derived from the Greek word meaning 'to deceive', in allusion to its similarity to other more valuable minerals, such as olivine, peridot and beryl.
Amongst other properties, apatite is said to enhance one's co-ordination and help to strengthen the muscles. Further, it has been credited with suppressing hunger and easing hypertension!
Apatite is commonly found in Burma (Myanmar), Brazil, India, Kenya, Mexico, Norway, Sri Lanka, East and South Africa, Spain, Russia, Canada, and the United States. Apatite found in Myanmar is quite light and ranges from colorless to light blue. Spanish apatite has a yellowish-green colour, and for this reason is often called "asparagus stone".


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Market Reports Center announces the addition of new study based research report on Compact Construction Equipments market to their suite of offerings.
Where the Compact Construction Equipments market is heading? If you are involved in Compact Construction Equipments sector, the report brings to your attention a basic overview of the Compact Construction Equipments market with market definition, classification, applications, segmentation, plans, manufacturing processes, product specifications, cost structures, regional analysis, and value chain analysis. Equipped with all vital stats and information with current scenario, insights, forecasts and future outlook, it offers highlights to foretell opportunities and challenges.
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The report features:
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Regional Insights:
The report lets you have an edge across the targeted regions with the comprehensive competitive framework. It analyzes the market on the basis of segmentation at a regional level coupled with price rate, profit, forecast, and estimates. The report studies the use of Compact Construction Equipments across several sectors to study and projects the future growth prospects. The report covers regional analysis of the market with respect to the existing market size and future prospects. It features historical stats, data and revenue estimation of the market segments and sub-segments in accordance with the top geographic regions and their countries. It discusses the current scenario of the Compact Construction Equipments market across major geographic segments, Europe, Southeast Asia and North America along with analysis of various country level United States, China, Japan and India markets for the demand of Compact Construction Equipments across each of these regions.
Competitive Landscape:
The Compact Construction Equipments market is characterized by the presence of a significant number of market participants. The research report lets you identify key organizations holding the greatest potential. Is also helps you stay ahead by figuring out capabilities, commercial prospects and progress of the key players. It also analyzes latest advancements in technology along with major industry participants profiled in the report. A review of macro and micro factors vital for the present market participants and new companies lets you evaluate competitive dynamics.
The commercial analysis and insights of Compact Construction Equipments market will let you stay well-versed with valuable business intellect on Compact Construction Equipments market.
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LADY LUMPS: First 'Female' Navy SEAL Candidate Might Not Even Be An Actual Woman At All
This Easy-to-Break Law Will Cost You $1 MILLION & 20 Years in Jail!
Mysterious, Gross, Yellow Fluff Balls Wash Up on French Shores
State of Emergency Declared in Hamburg, New York, After Tornadoes Leave Severe Damage
New Zealand storm: states of emergency declared as flooding hits South Island
VNAF F-5B "Rare" :) In Flight footage
YouTube Covertly Censoring Truth Channels
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BIN Takes You to the Most Infamous Crime Scene on Earth - Up Close and Personal!
The Illuminati/WW3/Luciferian Doctrine Coming (Video)
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Nobody Likes to Lose Fifty Dollars!
Windows 10 and Other Crap


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News By Tag
Industry News
* Arts
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Industry News
Meylah Releases Findings on Why Artisans Have Difficulty Selling Online
Study finds artisans want to sell online but time, tools, and expertise are still big hurdles
Key findings indicate that over 75% of the artisans surveyed are serious about their business and are involved either on a full-time or part-time basis, but less than half are currently selling their creative goods online. Of those who are not currently selling online, over 90% indicated they would like to bring their business online. When asked why they are not selling online, artisans pointed to three major hurdles:
1. Online Business Takes Too Much Time - The time it takes for an artisan to manage a blog, list their products in a marketplace and promote their business through social media in order to make sales takes significant time away from creating their work. One respondent mentioned, "By the time I finish promoting my business online, I feel like I have no time left to devote to actually creating my jewelry."
2. Online Solutions Are Not Easy To Use -The overwhelming majority said the challenges of selling online include finding the right tools, marketing their business, converting website and booth visitors to customers, and the amount of time required to actually make an impact. Another respondent said, "Taking my business online is confusing and I just don't know where to start."
3. Websites Are Too Expensive - 50% of artisans surveyed spend less than $250 per year in marketing their business. One artisan stated, "I spend over $200 each year on my website and it doesn't even allow me to sell through it, I know I'm losing sales because of this…it's so frustrating."
Meylah has a community of artisans who have successfully launched their own ecommerce websites and have grown their businesses substantially. With a Meylah website, artisans can sell their products (even digital products), blog about their work, engage with their customers and manage their business all in one place. "All artisans need the right tools to create: painters need brushes, musicians need instruments, and photographers need their cameras." says Ram Dutt, CEO and Co-Founder of Meylah. "That's why we built Meylah's ecommerce websites and marketplace, to give artisans the tools they need to sell their products anytime, anyplace...easily!"
About Meylah
Based in Seattle, WA, Meylah provides artisans with their own ecommerce website and powerful tools so they can sell their products anytime, anyplace…easily. Meylah'
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First Team News
Former City stars at Euro 2016: Part one
SLOVAKIAN HERO: Weiss scored a brilliant opener versus Russia on Wednesday
You're watching the Euros and suddenly you see a player that you recognise and may be think – 'didn't he used to play for us?'
Considering there are no less than 16 ex-Blues, representing 11 different nations, chances are your hunch is correct.
Of course, that won't be the case for higher profile players like Daniel Sturridge, Shay Given, James Milner and suchlike, but there are a large number of former Academy graduates playing for their nation at the championships in France.
So, without further ado, here is the first eight of the 16, complete with their City record and caps won…
Neil Taylor (Wales)
Another junior who never made it past junior level, the Swansea City left-back was at City from 1998 to 2005 and was released aged 16. He initially joined Wrexham before moving to the Swans where he has been for the past six years. He has 27 Welsh caps.
Shay Given (Ireland)
Popular Republic of Ireland Shay Given is still going strong. Now aged 40, he clocked up 69 appearances for the Blues between 2009 and 2011 and has won an astonishing 133 caps for the Irish.
Costel Pantilimon (Romania)
Costel Pantilimon played for City between 2011 and 2014 as back-up to Joe Hart. He made 29 starts in total and featured in the 2014 Capital One Cup final triumph over Sunderland. Costel has won 22 caps for Romania to date.
Wayne Hennessey (Wales)
Former City Academy youngster Wayne Hennessey was released by the Club as a 16 year-old, joining Wolves where he spent 11 years. He has won 57 caps for his country so far a well as making 239 career appearances at club level.
John Guidetti (Sweden)
Striker John Guidetti waited several years for his chance at City and was rewarded with just one appearance. Injury problems hampered his progress but he did enjoy successful loan spells with Feyenoord and is now progressing well with Celta Vigo. His strike partner for the national team is the one and only Zlatan Ibrahimovic and Guidetti has won 10 caps to date.
Andreas Isaksson (Sweden)
Something of a Swedish legend with 131 caps to his name, Andreas Isaksson spent two years with the Blues but never established himself with the Blues. He made just 20 appearances between 2004 and 2006 before moving to PSV.
Vladimir Weiss (Slovakia)
Part of the 2008 FA Youth Cup-winning team, Vladimir Weiss was tipped for big things at City. The Academy graduate played four times for the Blues, scoring against Arsenal in the League Cup, but eventually left to make his career elsewhere. He has had six clubs since and now plays his football in Qatar. He has already won more than 50 caps and scored against Russia on Wednesday.
Kieran Westwood (Republic of Ireland)
Kieran Westwood came close to breaking in the City team between 2002 and 2004, but never got his chance with David James and Nicky Weaver ahead of him in the pecking order. He eventually moved on and has had a solid career with Carlisle, Coventry City, Sunderland and Sheffield Wednesday. He has won 18 caps to date.
Can you name the other eight former Blues at Euro 2016? Tweet in @MCFC and see if you can beat us to them...


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San Diego Mount Soledad Memorial Cross
by Interanimate
|Daily Users
San Diego Mount Soledad Memorial. There are over 3,000 plaques honoring veterans, living and deceased, from the Revolutionary War to the current conflicts in the Middle East. The plaques on the walls tell the stories of scores of men and women from Presidents, Medal of Honor recipients, Admirals, Generals, Hollywood celebrities and thousands of others with names that are unfamiliar, but who proudly served our country to help protect the freedoms we enjoy as Americans.
More Causes Themes
live according to the Spirit
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Blue Lagoon Nebula with Mary and Jesus
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Black Hole Spirals
130 Daily Users
Nightly and Aurora - with V838 and Firefox
36 Daily Users
Thank you
As proud daughter of 33 year now-retired navy veteran and widow of an army man,
I very appreciate the themes for military / veteran
Thank you for sharing this and for your service if you are a veteran and for all veterans, thank you
Rated 5 out of 5 stars by Yax on August 28, 2013
To create your own collections, you must have a Mozilla Add-ons account.


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That's fine! Opened
Okay! That's fine Would you like to drop off or for me to pick up?
I have you added
Hi! Yes!! I'd love to buy! So, 1million total for 20?
Not a problem, I understand haha ^^ I paid you 60TBT
Hey, it's been a while ;; Do you still have the pokemon I paid for? Just figured I would ask~
Hope you've been enjoying the holidays!
Ok! I hope I didn't bother you, I just wanted to make sure neither of us forget to trade. I understand life is busy so please don't feel rushed ;;
What times will you be available Fri/Sat/Sun if any? o: Those are my most free days ^^
Not to be a bother, but just posting to remind us both that we need to trade ^^
Hey! Will you be able to trade me my Pokemon this week? o:
I forgot about my order!!! is it ready yet
Don't bother anymore In the video the girl was showing an extra Powersaves device which I don't have and don't intend to buy just to hack a game, so it was useless for me after all.
Thanks for the will, though!
Share your friend codes, gamer tags, and network IDs.
Meep meep!
Mayor of Utopia
Senior Member
Certified Floof
Junior Member


text:
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Voyeur : February 2009
Snapshots from... Y Hotel 'designed for your enjoyment' Sydney – City South, NSW Y Hotel City South is a very affordable, funky, boutique bed and breakfast hotel, in Sydney's hip inner city. ADDRESS PHONE FAX EMAIL WEB RATES 179 Cleveland St, Chippendale 61 2 8303 1303 61 2 8303 1300 email@example.com www.yhotel.com.au Rooms from $56 per person The Peninsula South Perth, WA Convenient access to shopping complex and restaurants. Perth CBD only 5 minutes by Ferry or car. STAR RATING HHHH ADDRESS PHONE WEB 53 South Perth Esplanade South Perth, WA 61 8 9368 6688 www.thepeninsula.net Kingsleys Steak & Crabhouse Sydney, NSW Canberra, ACT Brisbane, QLD Melbourne, VIC Grand Chifley Adelaide Adelaide, SA Grand Chifley Adelaide is a hotel of superior quality located opposite the picturesque South Terrace parklands, which enables guests to be close to the central business district yet in an environment that is welcoming and calm. ADDRESS 208 South Terrace, Adelaide, SA 5000 PHONE 61 (02) 9963 4300 WEB www.grandchifley.com Since 1994, Kingsleys Steak & Crabhouse has been offering diners superior standards of service and food in unique and outstanding locations with a relaxed and friendly atmosphere. Located in Sydney, Canberra, Brisbane and Melbourne (opening in November), once you have dined at Kingsleys Steak & Crabhouse, you will understand why diners keep coming back for more. Indoor and outdoor dining as well as private dining areas available in each location. OPEN 7 days lunch and dinner SYDNEY The Wharf, Cowper Wharf Rd, Woolloomooloo CANBERRA Ground Floor, North Quarter, Canberra Centre Civic BRISBANE Riparian Plaza, 71 Eagle St, Brisbane CBD MELBOURNE 91-93 Flinders Lane, Melbourne CBD PHONE 1300 KINGSLEYS (546 475) for a reservation WEB www.kingsleys.com.au Jet Ski Safaris Gold Coast, QLD • Self drive • No experience necessary • No licence required • Fully guided safaris • 1 or 2.5 hour safaris available or 1/2hour circuit. ADDRESS Waterways Drive, Main Beach PHONE 0409 754 538 WEB www.jetskisafaris.com.au SPECIAL $20 discout on presentation of this voucher. To feature your business in this section phone 1300 309 351
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cabrillo software9 links
- Cabrillo Web Forms
- Use these forms to manually enter log data to produce a Cabrillo file and submit it to a contest sponsor. The forms are designed for contesters who used paper logging or logging software that doesn't produce a Cabrillo file.
[ Hits: 573 | Votes: 2 | Rating: 7.00 ]
- HA1AH Contest Evaluator
- Contest organizers can use this software to receive e-mails containing contest logs written in the most common Cabrillo file format. The (automatic) evaluation of these Cabrillo files is also supported by creating summary and human readable log files.
[ Hits: 527 | Votes: 2 | Rating: 6.00 ]
- Contest LogChecker
- Contest LogChecker is a set of contest tools for Microsoft Windows, combining advanced text editing with keyword highlighted, and the ease and flexibility of import, export, statistics and charting utilities.
With LogChecker you can edit, fill in the log, check Cabrillo Format and Header, convert files from the most popular logging software to Cabrillo, print customizable files, calculate statistics and export logs to RTF, PDF or HTML for publishing.
You can examine a log in many different ways by highly interactive methods.
LogChecker includes a Master Callsign Database tool for managing databases, which have been generated from actual contest logs. Commercial
[ Hits: 2261 | Votes: 23 | Rating: 9.07 ]
- SH5 Contest Log Analysis
- SH5 is easy to use contest logs analyzer which creates a variety of HTML statistics from the Cabrillo format log generated by most popuplar contest log software (N1MM Logger+, Win-Test, Writelog, TR4W and others). SH5 allows you to upload contest results to your web site.
[ Hits: 1547 | Votes: 6 | Rating: 6.33 ]
- Cabrillo Evaluator by W3KM
- Contest adjudication software scores Cabrillo logs. Auto evaluates up to 2000 logs with duping, claimed score, actual score, cross checking, N-QSO rule, 5-min delta QSO rule. Support including code changes, non-scoring logs troubleshoot and summary output requests.
[ Hits: 1962 | Votes: 4 | Rating: 7.12 ]
- CBS - contest statistical software
- CBS is a program that produces statistical data from a Cabrillo file by K5KA and CBSW windows version by N6TV
[ Hits: 60 | Votes: 0 | Rating: 0.00 ]
- LogConv
- The new LogConv currently supports ADIF, Cabrillo, CT9, CT10, EQF and TR Log files as input and can output both ADIF and Cabrillo formatted files by ka5wss
[ Hits: 13224 | Votes: 18 | Rating: 4.89 ]
- Yea Cabrillo2Adif Converter
- Cabrillo2Adif A software for converting the radio logs from Cabrillo to Adif log format (for Mac OS X)
[ Hits: 714 | Votes: 0 | Rating: 0.00 ]
- Yea Cabrillo2Adif Converter
- Yea Cabrillo2Adif is a free software for converting the radio logs from Cabrillo to Adif log format for MacOSX
[ Hits: 110 | Votes: 0 | Rating: 0.00 ]
Not satisfied ? Lookup Google Custom Search Engine for cabrillo software
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Easy Paks® All-Purpose Cleaners; Deodorizer Packets, 90 Packets/Tub, 2 Tubs/Case
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Browsing Undergraduate Research Symposia by Author "Georgia Institute of Technology. Library and Information Center"
Now showing items 1-15 of 15
Building the House: Physical Implementation of Wood Working Marlatt, Drew (Georgia Institute of Technology, 2009-11-09)
Correlations Between Company's Investment in IT and Other Business Factors Hill, Keegan (Georgia Institute of Technology, 2010-11-18)
Experimental Plasmatron Heat Flux Measuring Techniques Study Vaden, Sarah (Georgia Institute of Technology, 2009-11-09)Ground testing for hypersonic re-entry vehicles is an invaluable methodology to vehicle conception. The Von Karman Institute plasmatron is a facility that can duplicate the localized aerothermodynamic properties and ...
Hemodynamic Assessment of Bicuspid Aortic Valves as a Clinical Diagnostic Tool Gupta, Shabnam (Georgia Institute of Technology, 2010-11-18)Bicuspid Aortic Valves (BAV) is the most common congenital cardiac defect occurring in approximately 1-2% of the newborn population in the USA. This defect results from the presence of two leaflets, typically unequal-sized, ...
Improvisation and Technical Innovation Lefton, Lew (Georgia Institute of Technology, 2009-11-09)
Inducing a thermogenic response to cold shock in Escherichia coli Graves,Christina; Holmes, Scott (Georgia Institute of Technology, 2010-11-18)Microorganisms respond to environmental stress through several means for survival. This research project aims to engineer Escherichia coli cells to generate heat in response to a cold-shock. Altering a cell's reaction to ...
Molecular midwives: The solution to strand cyclization in the pre-RNA world Chen, Michael (Georgia Institute of Technology, 2009-11-09)In recent years, the RNA-world hypothesis has become the leading theory for the origins of life. Few researchers, though, believe RNA could have spontaneously appeared out of the primordial soup. Regardless of the speculation ...
RNA Secondary Structure Prediction Anderson, Johshua; Ash, Andrew (Georgia Institute of Technology, 2010-11-18)Our task has been to calculate the probability of any two nucleotides pairing in an RNA molecule. When RNA goes into the cytoplasm of a cell, the RNA folds in on itself to a thermodynamically stable state, since unpaired ...
Study of Shear Induced Blood Damage in a Bileaflet Mechanical Heart Valve Bandari, Sahaja (Georgia Institute of Technology, 2010-11-18)Approximately 225,000 prosthetic heart valves are implanted each year worldwide, and about 50% of these are mechanical heart valves (MHVs). It is well established that present day MHVs are associated with a certain level ...
The Tower Weigel, Emily (Georgia Institute of Technology, 2009-11-09)What is The Tower? How to submit, work for, and find The Tower across campus.
The Tower Li, Henry (Georgia Institute of Technology, 2009-02-09)The Tower is an interdisciplinary research journal for undergraduates of all majors offered at Georgia Tech. The goals of our publication are to: showcase undergraduate achievements in research, inspire academic inquiry, ...
The Undergraduate Research Kaleidoscope Fall 2010 Chen, Michael (Georgia Institute of Technology, 2010-11-18)Individuals suffering from Friedrich's ataxia, a neuromuscular hereditary disease, have expansions of the DNA triplet repeat GAA/TTC in their genomic DNA. Preliminary investigations into the disease point to the ability ...
What Would Thoreau Do? Lesniewski, Victor (Georgia Institute of Technology, 2009-11-09)
Woodshop as Soulcraft Buffardi, Derek (Georgia Institute of Technology, 2009-11-09)
A Year at Walden Bohra, Kamna; Yellamraju, Siri (Georgia Institute of Technology, 2009-11-09)


text: The Illuminated wiki last edited by fables87 on 02/15/15 04:44AM View full history She took his side in the CIVIL WAR. Served under him as an agent of S.H.I.E.L.D. But when SHE-HULK finally uncovers what IRON MAN did to her cousin, she's going to do more than just break ranks... She's going to break every bone in his body! SHE-HULK'S most action-packed arc comes to a shattering conclusion! It's a battle for the ages! And one that will have terrible repercussions for Jen Walters, THE INITIATIVE, and THE INCREDIBLE HULK!This issue takes place right before Incredible Hulk #106She-Hulk # 18 concludes the "Planet Without a Hulk" storyline and with it, Jen Walters finally confronts Iron Man for his involvement in her cousin's disappearance!As an agent of S.H.I.E.L.D and a registered super-hero, Jen Walters serves under Tony Stark, but his actions against the Hulk have made her mad. And like any other green blooded Hulk family member (okay, the only other green blooded Hulk family member) you do not want to see her angry. She can be hard to handle when she's green.Writer Dan Slott, artist Rick Burchett, and cover artist Greg Horn bring you the undercard match of the year. Jen "the Savage She-Hulk" Walters vs. Tony "the Invincible Iron Man" Stark! And hey, if She-Hulk can't take Iron Man down, her cousin is up next!Unfortunately for Iron Man and the rest of the Illuminati, the Hulk isn't as far away as they would like. As a matter of fact…he's very close to home. Pick up She-Hulk # 18, as the Marvel Universe moves one step closer to the Hulk's return. Retailers are advised to check their orders on this issue as it directly ties in to the upcoming World War Hulk event.On the S.H.I.E.L.D. helicarrier, the Hulkbusters get word that a large gamma signature has been detected in New Mexico, the Hulk's old stomping grounds. The team has all assembled except for She-Hulk, who is not responding to the page. Clay Quartermain contacts Jen in her hover-car but she cuts off the transmission. She's tired of chasing after false leads. She has the head of the Nick Fury L.M.D. next to her. It's still busted and talking in a loop. She wishes she could think of a way to access its memory. She thinks about all of her friends that she could go to like Reed Richards, Hank Pym, etc. but she's not sure who she can trust now. Normally she'd go straight to Tony Stark but not now that he's in charge of S.H.I.E.L.D. She finally decides to go her lawfirm of Goodman, Lieber, Kurtzberg, and Holliway. It's there that Mr. Zix says he can access its data.She-Hulk sees a video of "Nick Fury" meeting with Bruce Banner. She finds out that the L.M.D. sent Hulk out on a space mission to "smash" something. They are unable to find out what happens next because the information is in a nested file. At the same time in a coffee shop across town, someone is using a laptop and mentions that "they've found the encrypted file..." On the laptop the screen says, "Operation: Greener Pastures, S.H.I.E.L.D. File 93.56.7."In New Mexico, the Hulkbusters arrive at one of the Hulk's old hideouts. They make their way inside and find the Leader inside. He is upset at being discovered. He mentions faking his death twice to carry out his secret master plan. He says he was just three days from releasing death spores. He gets in a giant robot and they prepare to fight.Zix tells Jen that he can't access any more files. Suddenly, a new file comes up. Back in the coffee shop, Mastermind Excello is seen with his coyote pup. He accessed the file for them. Jen sees another video with Tony Stark, Reed Richards, Black Bolt, and Dr. Strange talking to Hulk on a video monitor. They tell him that they decided to send him off planet when they found out about "Nick Fury's" mission. They say he has threaten the planet too many times with his anger-induced destruction. Seeing this, Jen is no longer a happy camper.The Hulkbusters suffer some injuries but they manage to take down the Leader. As they head back to the S.H.I.E.L.D. helicarrier, Clay's transmission is garbled. Those onboard the helicarrier think they are bringing in the Hulk and begin to celebrate. She-Hulk is also on her way to the helicarrier. Tony Stark is brought on deck and sees a huge banner saying "Mission Accomplished." He starts to talk to the agents that there's been a mistake (since he knows Hulk is not on planet). Jen arrives with a rough landing. She immediately confronts Iron Man. When he asks her what her problem is, she says her problem isn't on Earth and that he's known that from day one. Before she can say anything else, he hits her with a full-powered blast from his repulsors. He orders some agents to lock her in the brig and that she is NOT to talk to anyone. As he turns his back, She-Hulk has already regained consciousness. Stark orders her not to say another word. She easily dodges his attacks. She starts knocking him around and shrugging off his attacks. She calls him a "tin-plated tyrant" and says he's been acting more like Dr. Doom. She tells him he's an idiot. Sending Bruce into space isn't going to solve anything. Iron Man says he knows that he'll probably come back. He tells her that they've been preparing for his return. A little needle pops out of his finger and he pokes Jen in the neck. He tells her it's an Adamantium needle with nanites from Project: Achilles.When Jen wakes up, she immediately tries punching Iron Man only to feel pain in her fist. She has reverted back to her human form. Iron Man is showing her the results of Project: Achilles. She sees the Abomination, Ironclad, and Vector, all in human form. Stark tells her that they've perfected a way to stop Hulk-level threats, permanently. She-Hulk is no more. Jen tells him that he's mistaken if he thinks the threat from her has been neutralized. She-Hulk may be gone but she is still Jennifer Walters, one of the best lawyers on the East Coast. She says she is going to destroy him.continued in Incredible Hulk #106


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Summary of Season 1 (2004) - 4 discs
In this European cartoon series, civilization has been replaced by a feudal collection of floating islands -- and pesky dragons of all shapes and sizes roam everywhere. Setting themselves up as dragon hunters, Lian-Chu and Gwizdo embark on a string of adventures as they try to rid the land of the beasts. From a maternal Pink Horror who wants to take Lian-Chu under her wing to Gwizdo's dealings with an imposter, the excitement never stops.
Show Details
- Genres
- Children & Family, Kids' Anime, Cartoons, Kids' TV, Ages 8-10, Ages 11-12, France
- This show is
- Exciting, Family-friendly