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Cassini Spacecraft and Huygens Probe Mission
By: Anirudh V and Rahul G
What it does
Cassini basically examines Saturn, and it's moons(except the Titan since there is a probe for that called the Huygens). It uses all the instruments to examine Saturn, and is mainly there to check out the magnetosphere.
Why is it there?
Cassini is there because it is mainly built to examine Saturn's magnetosphere, and the second largest visible satellite.
The Cassini spacecraft was destined to orbit Saturn, and examine it. It also was supposed to release a probe called the Huygens that gathered data on Saturn's biggest moon, Titan(Titan was also the 2nd largest satellite in the solar system and had a very interesting atmosphere). Cassini was launched on October 15, 1997, but only reached Saturn on July 1,2004 since there is a great distance between the Earth and Saturn(746 million miles to be precise). It is a big effort for the NASA Jet Propulsion, European Space Agency(ESA) and the Italian Space Agency(ASI). Cassini was a very successful mission, and one of the greatest.
Dimensions and Weight
- The spacecraft is 6.7 meters high and about 4 meters wide, and weighs 2125 kg probe weighs 394 kg. At launch the mass of the spacecraft was about 5.82 tons and 3.1 of those tons were propellant which basically means that those were movable and practically engines.
Cassini Instruments
Cassini was obviously a powerful spacecraft, with lots of potency behind it. There were 18 instruments aboard the ship, 12 being a part of Cassini and 6 of the Huygens probe. A few of Cassini's focus on microwave remote sensing(which is it's communication to Earth), these are the Radar and Radio Science Subsystem (RSS).
The next part would be the optical remote sensing(this is where the instruments basically gathers data on Saturn, its rings and the moons through electromagnetic spectrum), these would be the Composite Infrared Spectrometer, Imaging Science Subsystem, Ultraviolet Imaging Spectrograph, Visible and Infrared Mapping Spectrometer.
The last category would be fields, particles and waves(these would be examining Saturn's surface), these would be the Cassini Plasma Spectrometer (CAPS), Cosmic Dust Analyzer (CDA), Ion and Neutral Mass Spectrometer (INMS), Magnetometer (MAG),Magnetospheric Imaging Instrument (MIMI),Radio and Plasma Wave Science (RPWS).
Huygens Probe Instruments
The Huygens probe was also pretty potent, but had to be more compact since it had to fit inside of Cassini in order to explore the Titan, and there were several instruments aboard it to keep it stable. The first is the two antennas part of the antenna subsystem, and each are responsible for a single job. The low end frequency is supposed to keep track with the Earth and keep stable communication, whereas the high one is supposed to act like an umbrella and keep the sun's harmful rays away. There are also three major RTGs (radioisotope thermoelectric generators) that give power to the probe, all the instruments, computers, radio transmitters, altitude thrusters and reaction wheels.
In all, Cassini has made a total of one mission, which it had succeeded in 2008. It has been so productive that it even has earned two more missions. One is current where it's supposed to gather more data for scientists to examine, and it's last will be in 2017 where it will crash into Saturn for the sake of looking at the surface. As for the probe, it couldn't survive that long without power, and therefore it is dead somewhere on Titan.
- "Cassini at Saturn." NASA. NASA, n.d. Web. 30 Apr. 2015.
- "Cassini Solstice Mission." Cassini Solstice Mission. Ed. Kirk Munsell. NASA, n.d. Web. 30 May 2015.
- Kurth, William S. "Cassini." World Book Student. World Book, 2015 Web. 30 April 2015.
- "Cassini Spacecraft." European Space Agency. ESA, n.d. Web. 01 May 2015.


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Wedding Packages
Planning a romantic Florida wedding and honeymoon is possible with Daytona Beach wedding and honeymoon packages at The Shores Resort & Spa.
Honeymoon at The Shores
Wedded bliss deserves the ideal honeymoon escape! Enjoy three amazing nights at The Shores - voted the #1 Resort for a Romantic Getaway by readers of the Orlando Sentinel. Your package includes Champagne and strawberries upon arrival, a couples massage and ritual bath in our private treatment room in the Indulge Spa and a $100 resort credit to enjoy in our signature restaurant Azure or for additional indulgences in our spa!
Create the Perfect Wedding
On behalf of the team at The Shores Resort & Spa, we congratulate you on your recent engagement and thank you for considering our premiere oceanfront property for your special day. The experienced event team stands ready to oversee even the tiniest detail of your once-in-a-lifetime event so you can relax and enjoy the company of your family and friends. To create your perfect wedding call us at 386.322.7248 for details and pricing or click the "Menus" tab above for more details.
Hot Summer Wedding Packages that are So Cool!
Get married at The Shores this summer and take advantage of some cool savings! The perfect place to enjoy your most special day. Our Summer Wedding Package includes our oceanfront - Ocean Terrace for your ceremony and a set-up for a cocktail reception for your guests for just $995! Call our Wedding Department at 386.322.7257 and explore ways to customize and enhance your wedding with receptions in our roof-top event space or our Grand Ballroom - whatever your dream wedding may be - our team of experts is here to make it a reality!

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text: Whether you wish to learn or perfect, the Megèvan ski school instructors are there to supervise you and ensure that you spend unforgettable times in their company. 8 results Modify search Filter ProfileI'm comingFromToadults count0 Adult1 Adult2 Adults3 Adults4 Adults5 Adults6 Adults7 Adults8 Adults9 Adults10 Adultschild count0 Child1 Child2 Children3 Children4 Children5 Children6 Children7 Children8 Children9 Children10 Children............CoupleFamilyWith friendsto............Revitalize yourselfExert yourselfClear search Add to journalSkiing Agency in Megève A skiing school of excellence. Expert skiing instructor in Megève. Individual lessons for all levels. A top quality personalized service. Lessons in French, English or Spanish. Add to journalRevolutionGlisse.com Looking for excellence, exclusive services … Welcome by your instructor, starting from your hotel or chalet. For half a day, one day, a week or more, you are our guest. Add to journalBureau des Guides The mountain guides company of Megève ("La Compagnie des Guides") was founded in 1969 by Henri Périnet. A warmful and ready to help team will welcome and offer you activities well-adapted to your wishes. Add to journalInternational Ski School A team of qualified and multi-lingual instructors, at your disposal to meet your demands. Add to journalEcole du Ski Français The Ecole du Ski Français has 7 different centers, in the center of the Village and down the slopes. Add to journalSkiing and Free-ride school Mountain guides. Ski instructors. Individual lessons for all levels. Adults and children from 3 years. High-quality personalized services. Skiing, snowboarding, parabolic, freestyle, off-piste skiing, heli-skiing lessons, White Valley…. Add to journalSandrine Janet Individual and personalized lessons for children with Sandrine, a highly qualified instructor Add to journalEvolution 2 Megève A different spirit. The shared passion for nature and sport, the desire for adventure, and for powerful and rewarding experiences. Experts in the field of adventure, with the desire to transmit our spirit: the quality of our services depends on the quality of our men.


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There's more to a military family than moving and deployments — take us along through each phase of your military life. And don't worry; we can help with the big stuff, too.
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Attention all members of a military family: Take a peek at our cheat sheet for all things career and education-related.
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Let us help you make the most of your new assignment from planning each step of your move to confirming that your new commissary does — in fact — stock your favorite kind of cereal. What would a new place be without a little adventure.
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The one constant of military life is that it changes — quickly. Be ready for the next chapter.
Stay Fiscally Fit During Deployment
Building and maintaining an emergency fund is a key to financial readiness for service members and their families. After covering your basic monthly expenses, start an emergency fund. It can save you a lot of stress and debt. Having that buffer allows you to focus on the mission, knowing that you and your family can handle life's curveballs. If an appliance breaks, the roof leaks or your car needs repairs, your emergency fund will provide peace of mind.
Set a goal for how much money you want to save for emergencies. A good general recommendation is three to six months of living expenses, although your first milestone could be less. You want to reach this goal fairly quickly, so aim for $500 to $1,000 to start.
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a road map for your money by taking the Developing a Spending Plan course to get your finances on track.
Involve your family in the process. Sit down for a meeting to develop a financial plan. Include the following steps:
You may think you can't squeeze an extra $25 for savings out of your weekly budget. Even if you have a tight budget, you can find small amounts to save. Try the following ideas for trimming your expenses:
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Once you've established an emergency fund, make a list of times when you would feel comfortable using it. Some people have difficulty recognizing that it's time to dip into their emergency savings and instead pull out their credit cards, which leads to debt. Once you've used money from your fund, you must set a goal to replenish it through weekly savings so that you can cover the next emergency. Take comfort in knowing that you can provide for your family even in a time of crisis without going into debt.
Learn more about financial readiness by visiting your installation's Personal Financial Management Program or call Military OneSource (800-342-9647) for free financial counseling, education on money-related topics and assistance with budgeting and saving. A financial counselor can confidentially review your situation and recommend how you can take advantage of Department of Defense resources.
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Teaching Group Classes for cellists
Jul 2 – 7, 2017 in Deerfield, IL
at Chicago Suzuki Institute
Teaching Group Classes for cellists: Cello
Instructor: Carey Beth Hockett,
Group teaching is an essential skill for every Suzuki teacher. Through a combination of "hands on" group class teaching and lively discussions, participants will leave with a wealth of teaching ideas and deeper appreciation of the impact of the group class experience on the Suzuki student.
This is a supplement to the unit courses. Participants should be conversant with the Suzuki repertoire and have registered units through book 4.
Teachers who have completed the group class course may wish to return as a fellowship student. Please write [javascript protected email address] for more information on the Group class for cellists fellowship program.
Prerequisite: All books covered in the overview course.
Audition: Cello Basic, or Cello Intermediate, or Cello Comprehensive.
Deadline: All audition materials must be submitted by July 2 *
*A discounted Audition Fee applies if audition is submitted before May 7. If submitted within 8-weeks of the course start date, the regular audition fee will apply.
Signup closed.
Carey Beth Hockett has been actively involved in the Suzuki world for more than 30 years. She taught at the Ithaca Talent Education School and then for the London Suzuki Group and has recently joined the faculty of the Colburn School in Los Angeles. She was a member of the International Suzuki Association's Cello Committee and served as repertoire consultant to the Associated Board of Royal Schools of Music. She has given numerous workshops for teachers and children across Europe and America is well known for her work with groups of all sizes. She is the founder of Corkybird Enterprizes, which produces creative designs for music education.
Sunday, July 2 to Friday, July 7, , 2017
Chicago Suzuki Institute
Trinity International University
Deerfield IL
Phone: 847-905-1500
[javascript protected email address]
More Info & Contact
Website: www.chicagosuzukiinstitute.org
Blake Brasch, Admin. Director
Phone: 847-905-1500 ext. 511
Fax: 847-446-3876
[javascript protected email address]
The SAA does not run the workshops and summer institutes; it approves separate businesses to run them. For specific information on schedules, enrollment, pricing, housing, etc. please contact sponsoring organizations directly with inquiries. To get your event listed here, please see the Event Listing Guide.


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1965 Chevrolet Sport van
Price: US $3,600.00 Item location: Saint Augustine, Florida, United States
- Condition: Used
- Make: Chevrolet
- Model: Other
- Year: 1965
- Mileage: 31,831
- Vehicle Title: Clear Want to buy? Contact seller!
1965 Chevrolet OtherA very cool van for sale !!!
Can be driven. New brakes and muffler. 6-cylinder, riginal interior, indows, mblems, og dish hubcaps.
Has original owners manual and information about the first owner in the glove box.
As you can see from the pictures restoration is underway. The major rust has been cut out and metal was welded in, ondo-ed and primed.
Headliner and interior panels are bare. Was originally used as a cargo van so there are no benches or seating in the back.
Ready for someone to take to the next step.


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Pizzeria Bianco Map
623 E. Adams St. (Seventh St.)
602-258-8300 | Make Restaurant Reservations | Menu
Find Pizzeria Bianco with this handy map to the restaurant. If you don't see a pop-up, click on the map's green arrow to get driving directions to and from Pizzeria Bianco.
Find out which restaurants and chefs top the list of GAYOT's 2016 Annual Restaurant Awards.


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The winner will go through to the - on paper - easier group which contains New Zealand, Pakistan and South Africa.
Hosts India, world champions Australia and England await the losers and both captains say they are desperate to win.
Sri Lanka recalled seamer Chaminda Vaas for Ruchira Perera, while Fidel Edwards and Dwayne Smith replaced pacemen Ian Bradshaw and Jerome Taylor for Windies.
Windies suffer early blow: Windies suffered early blow when they lost their batting mainstay Chris Gayle in the very first over edging a regulation catch to Sangakkara off Chaminda Vaas.
Ramnaresh Sarawan soon joined him in the pavilion when he was trapped plumb infront for nought off Vaas. And as though this was not enough, Windies lost its third wicket in the form of struggling Chanderpaul who edged Malinga to the wicket keeper Sangakkara to do the rest, leaving Windies at 17/3 in 7 overs with Skipper Lara (2) and Hinds (1)were at the crease
Sri Lanka: Mahela Jayawardene (capt), Kumar Sangakkara, Sanath Jayasuriya, Marvan Atapattu, Upul Tharanga, Tillkaratne Dilshan, Farveez Maharoof, Chaminda Vaas, Dilhara Fernando, Lasith Malinga, Muttiah Muralitharan.
West Indies: Brian Lara (capt), Ramnaresh Sarwan, Chris Gayle, Shivnarine Chanderpaul, Dwayne Bravo, Dwayne Smith, Carlton Baugh, Corey Collymore, Fidel Edwards, Marlon Samuels, Wavell Hinds.
Umpires: Asad Rauf (PAK) and Rudi Koertzen (RSA)
TV umpire: Mark Benson (ENG)
Match referee: Mike Procter (RSA)


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Hi everyone, my girlfriend and I are taking a last minute trip to London. Neither of us have been, but we are very excited to experience it.
Since it's so last minute, I've not really had time to research restaurants. While we don't have an incredible amount of money to spend, I would like to go at least one pretty nice place (like around $250 US total).
Can anyone recommend a romantic place with great food where we can either get short notice reservations or don't need reservations at all?
Any other thoughts are appreciated. We're staying near Charing Cross station.
Thanks in advance.


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Divorce Lawyer in Love (K-Drama) 2015
Sinopsis Drama Korea Divorce Lawyer in Love (K-Drama) 2015 : Go Cheok Hee is a successful divorce lawyer who treated So Jung Woo terribly when he was her firm's office manager. The tables are turned when Cheok Hee loses her license and can no longer practice law and Jung Woo has become a successful lawyer. The two cross paths again when Cheok Hee becomes Jung Woo's office manager.
- Title: 이혼변호사는 연애중 / Yihonbyunhosaneun Yeonaejoong
- Genre: Romance, comedy
- Episodes: 18
- Broadcast network: SBS
- Broadcast period: 2015-Apr-18 to 2015-Jun-14
- Air time: Saturday & Sunday 21:55
- Original Soundtrack: Divorce Lawyer in Love OST
- Jo Yeo Jung as Go Cheok Hee
- Lee Bit Na (이빛나) as young Go Cheok Hee
- Yun Woo Jin as So Jung Woo
- Shim Hyung Tak as Bong Min Kyu
- Wang Ji Won as Jo Soo Ah
- Extended Cast
- Lee Yul Eum as Woo Yoo Mi
- Hwang Young Hee as Yoon Jung Sook
- Lee Dong Hwi as Lee Kyung
- Maeng Sang Hoon as Go Dong Sang
- Park Joon Geum as Ma Dong Mi
- Lee El as Han Mi Ri
- Cha Yub (차엽) as Jo Yoo Sang
- Yang Ji Won (양지원) as Yoo Hye Rin
- Kim Yul as Go Mi Hee
- Sung Byung Sook as Jang Mi Hwa
- Kim Gab Soo as Bong In Jae
- Shin Ha Yun as Lee Ha Jung
- Son Se Bin (손세빈)
- Lee Joon Hyuk
- So Hee Jung
- Sun Woo Sun
- Kim Ik Tae
- Jung Eun Pyo
- Jun Soo Kyung
- Son Sung Yoon
- Kwak Ji Hye
Production Credits
- Director: Park Yong Soon
- Screenwriter: Kim Ah Jung (김아정)
Download Divorce Lawyer in Love (K-Drama) 2015
Download Link
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Members' Tour | International Contemporary Furniture Fair with Matilda McQuaid
Cooper Hewitt, Smithsonian Design Museum
Saturday, May 17, 2014 from 12:00 PM to 1:30 PM (EDT)
Event Details
Preview the 26th Annual International Contemporary Furniture Fair with Matilda McQuaid, Deputy Curatorial Director.
More than 500 exhibitors from all points of the globe will display contemporary furniture, seating, carpet and flooring, lighting, outdoor furniture, materials, wall coverings, accessories, textiles, and kitchen and bath for residential and commercial interiors.
Open to Design Watch Members and above. RSVP online or to the Membership Office at 212.849.8349 or firstname.lastname@example.com.
Not a Design Watch Member?
Join, renew, or upgrade today and receive 2 years of Membership for the price of 1.
When & Where
Jacob K. Javits Convention Center
11th Avenue at 38th Street
New York,
Saturday, May 17, 2014 from 12:00 PM to 1:30 PM (EDT)
Add to my calendar


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Julian Assange Extradition
November 2, 2011 by staff
Julian Assange Extradition, The founder of Wikileaks, Julian Assange, lost his appeal against extradition to Sweden to respond to allegations of sex crimes, but said the night was to consider the possibility of prolonged struggle at the highest court in Britain.
Superior Court of appeal judges John Thomas and Duncan Ousely Assange rejected claims that it would be unfair and illegal to send him to Scandinavia for questioning about the alleged rape of a woman and sexual abuse of another in Stockholm last year. The 40-year-old has denied any wrongdoing and insists the case is politically motivated by those opposed to the work of spilling his secret organization.
"We will consider next steps in the coming days," said Assange off the court. It did not seem angry or visibly upset, despite significant judicial setback.
Attorney Mark Summers confirmed that it was unclear whether his client an attempt to appeal to the Supreme Court of Great Britain. His legal team has 14 days to decide whether to apply to the Supreme Court, then you must try to persuade the judges that there is a point of law to justify an appeal to higher court.
This means that Assange will remain in Britain for at least several weeks and could extend his fight against extradition in the next year. At a hearing, appellate judges confirmed that Assange remain free on bail, under house arrest in a friend's field in southern England.
Legal experts insisted that the odds are stacked against Assange to avoid extradition to Sweden.
"I think it is very likely going to be in Sweden before the end of the year," said Julian Knowles, an attorney involved in the extradition case.
Vaughan Smith, the owner of the mansion where Assange is living, said his friend's prospects looked bleak. "It's not good news," he told The Associated Press.
Smith said Assange is concerned about the impact on your organization if sent to Sweden, for fear they may be held in jail as contests of the charges against him.
"How can you run a prison WikiLeaks? You can not," said Smith. "There is good reason for him not to go to Sweden."
Also unclear is whether Assange has the resources to fund a legal battle continues. In a recent dispute over his autobiography, whose draft was published without his permission, the founder of Wikileaks revealed that he had been with their previous lawyers about the size of his bill and did not have enough money to sue their publishers.
Assange and his supporters say he is not using WikiLeaks funds for his defense.
In its ruling, the appeal judges rejected the key arguments Assange legal team. He said Sweden had the right to issue a warrant for Assange, rejected claims that the alleged offense had been wrongly described, dismissed questions about the process of Sweden for instigating criminal investigations, and ordered that prosecutors had provided in their actions.
"This is obviously a case involving a trivial offense, but for serious sexual offenses," the judges wrote in his ruling, upholding the original decision of the court in February that Assange should be extradited.
Assange has said that sexual encounters were consensual, and his lawyer, Ben Emmerson, had previously held that the allegations would be considered crimes in England.
The appellate judges said that the apparent inconsistencies in some of the accusations against Assange should not affect his extradition for questioning despite the problems could be valid in any future trial. They cited as an example the fact that a woman who claimed Assange had unprotected sex with her against her will while she slept was later said could have been partially awake.
"These are issues that would be highly relevant evidence in the trial," the judges wrote. But "not for this Court to determine whether the allegations can fail."
Assange a step closer to extradition, is an open question whether your site can survive.
WikiLeaks finances are under intense pressure and some of its biggest revelations are in the public domain. Only Assange warned last week that the site was so low on cash losses would have to stop the publication and may close completely in two months, unless funding improves.
Assange also faces possible legal action in the United States, where prosecutors are considering possible criminal charges.
Bradley Manning, the U.S. Armyanlyst suspected of disclosing classified information to WikiLeaks, remains in custody in prison at Fort Leavenworth in Kansas. His case is pending before a military tribunal.
Please feel free to send if you have any questions regarding this post , you can contact on
Disclaimer: The views expressed on this site are that of the authors and not necessarily that of U.S.S.POST.


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Who Is Zo Endorsing for President? | ZoNation
The question the whole wide… Neighborhood wants the answer to is, "Who am I pulling for for president." Hear my response in this ZoNation!
Please contribute to my Geeky but Cool Sci-Fi invention! Seriously! This is gonna be HUGE! If you really support my work and want to help keep my work going, please contribute! CLICK THE IMAGE!
Thanks for watching my vids! If you like the message in them then You'll have a BLAST nukin' the liberal Narrative with my audio book of Christian Conservalicious profundus, written and read by Me! WEAPON OF A.S.S. DESTRUCTION! CLICK HERE OR IMAGE AND CHECK OUT SOME REVIEWS, AND GET YOUR COPY!!!
Hope you'll Visit my band's Facebook page and CLICK LIKE, if you'd like to see more artists who are "One Nation under God patriots" instead of people who just wanna whine with a chip on their shoulder!
Trending on AlfonzoRachel.com
The Destruction of American Culture, Values and Traditions by the Left
Unlocking the Truth and Blocking the Truth
The Negative Effect of Illegal Immigrants
Just Born This Way. Gay and Gangsta
Trump Won. Are Those liberal Celebrities Still Leaving?
Comey Pointing Weiner at Hillary
George Zimmerman Is Not White, Unless You Are Liberal (Warning: Graphic Images)
Is Halting Muslims from U.S. Entry Racist? : ZoNation
Bible Trippin 045 Genesis 16 with AlfonZo Rachel
ZoNATION: Live MLK's Dream, And Get Called 'White Man's Whore' For It
ZoNation Vid: Stephanie Miller; A Liberal Licensed Racist
Carrie Fisher Autopsy Reveals More Than Drugs
From Brock Lawley: Open Letter To Dr. Benjamin Carson
The Zo Loft : Democrats, Refugees, and Discrimination
The Zo Loft : Liberals, Selective Christianity, & Refugees
My Observations on the Trump / Hillary Debates by AlfonZo Rachel


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Fluorescence-activated cell sorter analysis showing up-regulation of isotype control (A), CD86 (B), I-Ak (C), intercellular adhesion molecule-1 (D), and OX40L (E). This demonstrates a mature dendritic cell phenotype after an 8-day culture. FL2H indicates red fluorescence (phycoerythrin-conjugated monoclonal antibodies to cell markers).
Growth of naïve squamous cell carcinoma VII (SCCVII) and irradiated SCCVII cells (5000 cGy) after subdermal inoculation (1 × 106). This demonstrated that 5000 cGy was sufficient to prevent SCCVII tumor growth.
Fluorescence-activated cell sorter analysis demonstrating dual staining of fusion cells in the upper right quadrant. Tumor cells are labeled with carboxyfluoroscein diacetate succinimidyl ester (FL1H) and with phycoerythrin-conjugated antibody against dendritic cell (DC) markers (FL2H) (A); a DC–tumor cell mixture is shown as a negative control (B).
Geimsa staining of dendritic cell (DC)–squamous cell carcinoma VII (SCCVII) fusion cells that shows multinucleated cells suggesting successful DC-SCCVII fusion (A) (arrows); a DC–SCCVII cell mixture is shown as a negative control (B) (original magnification × 40).
Growth of squamous cell carcinoma VII after treatment of 3-day established subdermal tumors. Treatment with fusion cells + adjuvant treatment significantly inhibited tumor growth (*P < .001). There was no significant difference between using interleukin (IL)-12 or anti-OX40R monoclonal antibodies (mAbs) as a fusion cell adjuvant treatment. Error bars indicate SD.
Survival of mice after treatment of 3-day established subdermal tumors. Mice treated with fusion cells + adjuvant treatment or fusion cells alone demonstrated a survival advantage over control groups. Survival for fusion cells + OX40R monoclonal antibodies (mAbs) and fusion cells alone were 60% and 40%, respectively.
Survival of mice after treatment of 3-day established pulmonary metastases. Mice treated with fusion cells alone and fusion cells with anti-OX40R monoclonal antibodies demonstrated an 80% and 100% survival, respectively.
Lee WT, Tamai H, Cohen P, Teker AM, Shu S. Immunotherapy of Established Murine Squamous Cell Carcinoma Using Fused Dendritic-Tumor Cell Hybrids. Arch Otolaryngol Head Neck Surg. 2008;134(6):608-613. doi:10.1001/archotol.134.6.608
© 2017
To investigate the therapeutic efficacy of fused dendritic-tumor cell hybrids against murine squamous cell carcinoma (SCC).
Squamous cell carcinoma VII is a poorly immunogenic murine SCC tumor in C3H/HEN (H-2K) mice. Subdermal tumors were established by inoculation in the mid abdomen of mice. Tumor diameters were measured with a Vernier caliper and used as an indication of treatment efficacy. Survival studies were performed on mice with 3-day pulmonary metastasis or subdermal tumors. Dendritic cells were generated from bone marrow and cultured for 8 days. Dendritic cells were harvested and mixed with cultured tumor cells in a 1:1 ratio. Cell fusion was achieved by exposing the cell mixture to an alternate electrical current to bring cells into alignment and close together, followed by a short direct electrical current pulse.
Female C3H/HEN mice aged 8 to 12 weeks.
Mice with 3-day established SCCVII tumors were vaccinated by inguinal intranodal injection of fusion cells (0.3 × 106 per side). To support the development of antitumor immunity, mice were given adjuvant injections intraperitoneally. Anti-OX40R monoclonal antibodies or interleukin 12 were used. Treatment groups included no treatment, anti-OX40R monoclonal antibodies or adjuvant IL-12 alone, fusion cells alone, and fusion cells with adjuvant treatment.
Main Outcome Measures
Tumor size and overall survival.
Mice treated with adjuvant treatment or fusion cells alone did not show a statistical difference in tumor growth when compared with controls. In contrast, fusion cells with adjuvant treatment demonstrated a significant decrease in tumor size when compared with nontreated mice (P < .001). Treatment with fusion cells also resulted in increased survival in the pulmonary metastasis and subdermal tumor models.
Immunotherapy with fused dendritic-tumor cell hybrids can significantly affect 3-day established sSCC VII tumor growth.
Head and neck squamous cell carcinoma (HNSCC) comprised an estimated 39 000 newly diagnosed cases and 11 000 deaths in the United States during 2005.1 Over the past few decades, the treatment of HNSCC has seen advances in surgical techniques, radiation modalities, and chemotherapy regimens. Advanced-stage HNSCC often requires a combination of these treatment options, and these advancements have led to organ preservation and an improvement in locoregional control.2- 4 Despite these improvements, the primary cause of cancer failure remains distant metastatic disease.5
One approach to addressing this issue is immunotherapy. The goal of immunotherapy is to sensitize T cells to recognize and kill tumor cells. This approach would be especially useful in addressing distant metastatic disease in which current treatment options are not as effective. Within the past decade, studies have identified the dendritic cell to be a critical component in eliciting a T-cell response against neoplastic cells.6- 9 Dendritic cells are the most specialized antigen-presenting cell known because of their unique characteristics to uptake, process, and present antigens to T cells. Because of this, immunotherapy approaches have been focused on using the properties of dendritic cells. One such approach is the use of fused dendritic-tumor cell hybrids created by electrofusion.
Electrofusion is a process in which 2 different parent cells are fused together to form a new hybrid cell. This hybrid cell retains properties of both parent cells. In the case of dendritic-tumor cell fusion, the new hybrid cell retains the antigen-presenting properties of the dendritic cell to present the full complement of antigens from the tumor cell. This would include both known and unknown tumor antigens. Fused dendritic-tumor cell hybrids have been shown to have immunotherapeutic effects after a single vaccination in a number of murine carcinomas including breast cancer, sarcoma, and melanoma.10,11 The immunotherapeutic efficacy induced by these fusion cells were dependent on the presence of both CD4+ and CD8+ T cells.11 An important finding was the need for an adjuvant treatment for these effects to be seen. Neither adjuvant treatment alone nor fusion cells alone were able to achieve similar results.
To date, no preclinical studies, to our knowledge, using immunotherapy with dendritic-tumor cell fusion against squamous cell carcinoma (SCC) have been reported. Investigation of the immune mechanisms induced by fusion vaccines in a murine SCC model will help develop future clinical trials. Our hypothesis is that a fused dendritic-tumor cell hybrid can induce an immunotherapeutic effect against established murine SCC tumor.
The Animal Research Committee of The Cleveland Clinic approved this study. All animals used in this study received humane care in compliance with the "Guide for the Care and Use of Laboratory Animals," as outlined by the National Institutes of Health. Institutional guidelines regarding animal experimentation were followed. The Cleveland Clinic Animal Care facility is accredited by the American Association for the Accreditation of Laboratory Animal Care.
Female C3H/HEN (H-2k) mice were purchased from the Biologic Testing Branch, Frederick Cancer Research and Development Center, National Cancer Institute, Frederick, Maryland. The animals were housed in a specific pathogen-free environment and were used for experiments at the age of 8 to 12 weeks.
Squamous cell carcinoma VII (SCCVII) is a spontaneously arising SCC tumor in C3H/HEN (H-2K) mice. Despite the expression of MHC class I molecules, it is poorly immunogenic and highly metastatic.12 Squamous cell carcinoma VII cells were maintained in complete medium made of Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% heat-inactivated fetal calf serum, 2mM levoglutamide, 0.1mM nonessential amino acids, 1mM sodium pyruvate, 100 μg/mL of streptomycin sulfate, 100 μg/mL of penicillin G potassium, 0.5 μg/mL of amphotericin B, 50 μg/mL of gentamicin sulfate (all from BioWhittaker, Walkersville, Maryland) and 50μM 2-mercaptoethanol (Sigma, St Louis, Missouri). Cultured SCCVII cells were harvested after brief exposure to 0.25% trypsin and 0.02% EDTA (BioWhittaker). Tumor cells were washed twice and resuspended in Hanks balanced salt solution for animal inoculation. Before fusion, tumor cells were exposed to 5000 cGy of radiation to prevent in vivo replication and stained green with intracellular carboxyfluoroscein diacetate succinimidyl ester according to manufacturer's instructions (Molecular Probes, Eugene, Oregon).
Dendritic cells were generated by harvesting the fibula and tibia bone marrow of 8- to 12-week-old C3H/HEN mice. Unfractioned bone marrow cells were cultured for 6 days in a proliferative cocktail of FMS-like tyrosine kinase 3 (10 μg/mL) and interleukin (IL)-6 (10 μg/mL). On day 6, cells were harvested and placed into complete medium containing granulocyte-macrophage colony-stimulating factor (10 μg/mL) and IL-4 (10 μg/mL). Toll-like receptor agonists lipopolysaccharide LPS (50 ng/mL) and CpG (5μM) were added to the culture on day 7. The cells were harvested, counted, and used for fusion 24 hours later.
Dendritic cells and irradiated (5000 cGy) tumor cells were first washed in a prefusion media of 5% glucose, 0.5mM magnesium acetate tetrahydrate, 0.1mM calcium acetate tetrahydrate, and 0.3% bovine serum albumin (pH 7.2) at a ratio of 1:1. Cells were then resuspended in fusion media of 5% glucose, 0.5mM magnesium acetate tetrahydrate, and 0.1mM calcium acetate tetrahydrate. Routinely, total cell concentration was between 10 × 106/mL to 15 × 106/mL.
Electrofusion was performed using the ECM 2001 generator (BTX Instrument Division, Genetronics, San Diego, California). A custom designed concentric fusion chamber was used to hold and fuse the cell suspension. Alignment of the cells was accomplished by applying an alternating current (160 V/cm) to the suspension for 10 seconds. Alignment was confirmed with direct visualization using a microscope. Immediately following the alternating current application, a direct current pulse (1533 V/cm) was applied for 99 microseconds. The cell suspension then underwent a tapering of the same alternating current for 9 seconds.
The fusion cells were harvested and incubated overnight in complete medium. After the cells were cultured overnight, adherent cells were harvested after brief exposure to trypsin and suspended in Hanks balanced salt solution for in vivo use. Verification of fusion efficiency was performed using fluorescence-activated cell sorter (FACS) analysis. After fusion, adherent hybrid cells were then labeled with the red phycoerythrin-conjugated monoclonal antibodies to cell markers found exclusively in dendritic cells. Since tumor cells were stained green with carboxyfluoroscein diacetate succinimidyl ester, fused dendritic-tumor cell hybrids are recognized by double-positive staining by FACS analysis.
Tumors were established subdermally by injection of 0.5 × 106 SCCVII cells into the mid abdomen. After 3 days, fusion tumor vaccine was given by intranodal route. This was performed while the animal was anesthetized with 0.8 mg of pentobarbital intraperitoneally. The inguinal lymph nodes were surgically exposed, and 0.3 × 106 fusion cells in 10 μL per side were injected intranodally with a 29-gauge needle. The surgical wound was reapproximated with surgical clips. Once mice recovered from anesthesia, a vaccine adjuvant anti-OX40R monoclonal antibody (mAb) (150 μg × 1 day) or IL-12 (0.2 μg/d × 4 days) was administered intraperitoneally. Perpendicular tumor diameter was measured at regular intervals with a Vernier caliper. Size was then recorded as the mean of these 2 measurements. Mice were humanely killed when tumor size reached a mean of 15 mm according to animal handling guidelines.
Pulmonary metastases were established by injection of 0.3 × 106 SCCVII into the tail vein of mice. After 3 days, the fusion tumor vaccine was delivered in the same manner as previously described. Mice were then monitored on a routine basis to measure survival.
The experiments described herein are designed to evaluate the therapeutic efficacy of immunotherapy by vaccination with dendritic-tumor cell hybrids. Differences in the tumor size between experimental groups were evaluated with the nonparametric Wilcoxon rank-sum test (SigmaStat; Systat Software Inc, San Jose, California). Thus, the likelihood of the observed results being due to chance alone was determined. P ≤ .05 was considered statistically significant. Each tumor growth group consisted of 5 animals. This is the minimum number of mice for which 1 overlap may be considered significant. According to the proposed statistics, in groups consisting of 5 animals, significance would be achieved in overlaps of 0 or 1 between experimental groups. However, if only 4 animals per experimental group are used, significance may only be achieved without any overlap whatsoever.
After the 8-day dendritic cell culture as described in the "Methods" section, we were able to generate a mean of 30 × 106 cells per mouse. These cells demonstrated high expression of molecules typical of matured dendritic cells (CD86, I-Ak, intercellular adhesion molecule-1 [ICAM-1], and OX40R). The up-regulation of these molecules demonstrate that these mature dendritic cells are especially equipped to present antigen to the immune system.8,11 The FACS analysis is shown in Figure 1.
Before performing therapeutic experiments using dendritic and tumor cells, the capacity of irradiated (5000 cGy) SCCVII to proliferate in vivo was investigated. A total of 1 × 106 cells of either live or irradiated SCCVII cells were inoculated subdermally. Figure 2 shows the growth of these cells over time. Animals inoculated with irradiated tumor showed tumor regression after an initial limited growth. These animals had no signs of tumor at 6 months after irradiated tumor inoculation.
Dendritic–SCCVII tumor cell fusion was performed as described in the "Methods" section, and fusion rates were routinely above 20%. Fusion was confirmed using FACS analysis. Tumor cells were stained with the green fluorescent carboxyfluoroscein diacetate succinimidyl ester before fusion. Fusion cells were then analyzed by exposure to red fluorescent phycoerythrin-labeled antibody to exclusively dendritic cell surface markers. Cells that expressed dual fluorescence represent fused dendritic-tumor cells. The FACS data of a nonfused dendritic-tumor cell mixture as a negative control is shown for comparison (Figure 3).
Fusion cells were stained with Giemsa to allow for direct visual confirmation of successful fusion. Multinucleated cells represent successful fusion. Figure 4 is a Geimsa-stained cytospin preparation of fused dendritic-SCCVII cells. A Geimsa-stained cytospin preparation of a dendritic-tumor cell mixture is shown for comparison.
The treatment of 3-day established SCCVII subdermal tumor was investigated. Treatment groups included no treatment as a negative control, IL-12 alone, anti-OX40R mAbs alone, fusion cells alone, fusion cells + IL-12, and fusion cells + anti-OX40R mAbs. Subdermal tumor growth is shown in Figure 5. Only fusion cells + IL-12, and fusion cells + anti-OX40R mAbs consistently demonstrated a significant difference when compared with the nontreated group (P < .001). All results were confirmed in repeated experiments. Figure 6 demonstrates long- term survival in mice after fusion vaccine treatment of 3-day established subdermal tumors. Survival of mice treated with fusion cells + OX40R mAbs and fusion cells alone were 60% and 40%, respectively.
Survival of 3-day established SCCVII metastases was investigated because this more closely mimics the clinical scenario of HNSCC metastasis. Treatment groups included no treatment as a negative control, anti-OX40R mAbs alone, fusion cells alone, and fusion cells + anti-OX40R mAbs. Mice treated with fusion cells + OX40R mAbs and fusion cells alone demonstrated survival of 100% and 80% at 75 days, respectively. No mice in the control group and adjuvant treatment only group survived beyond 43 and 60 days, respectively (Figure 7).
These data represent the first immunotherapy treatment model against murine SCC using fused dendritic-tumor cell hybrids. The use of fused dendritic-tumor cell hybrids with an adjuvant treatment (IL-12 or anti-OX40R mAbs) were shown to significantly affect tumor growth when compared with experimental controls that included fusion cells alone, adjuvant treatment alone, and no treatment. We theorize that the use of an adjuvant treatment demonstrated improved efficacy when compared with fusion cells alone because the success of an immune response depends on 3 signals. The first is the presentation of the antigen on the major histocompatibility complex molecule to the antigen receptor on the T cell. The second signal is ligation of costimulatory molecules such as CD80 and CD86. The third signal is supplied by the adjuvant treatment, which appears to be important in developing an effective antitumor response. Furthermore, significant survival of mice was seen when given fusion cells with or without OX40R mAbs when compared with controls. Although there was some effect seen with fusion cells alone in the pulmonary metastases model, there is an increase in efficacy when fusion hybrids are given with OX-40 mAbs.
The use of IL-12 and anti-OX40R mAbs as adjuvant treatments were based on previous studies using fusion cells in other tumor models. The mechanism of action was described in a recent publication that found IL-12 and OX40R ligation leads to interferon γ secretion from T cells. Although the fusion cells strongly provide signals 1 and 2, without the addition of the third signal supplied by the adjuvant treatment, T cells may not expand and sustain the antitumor response.13
Furthermore, we report the feasibility of using a new method of dendritic cell generation from bone marrow. This process was modified from the previous bone marrow–derived methods and involves proliferating DC in FMS-like tyrosine kinase 3 ligand and IL-6 for 6 days. Further maturation in vitro is done using the toll-like receptor agonists LPS and CpG 24 hours before fusion. These dendritic cells demonstrated fusion capacity with SCCVII and immunotherapeutic effects against established tumors. This new dendritic cell culture method may potentiate the immunotherapeutic effects of fusion cells alone, since there were significant effects noted as compared with controls. This effect of fusion cells alone was not observed in other tumor models using traditional dendritic cell culture methods.13
One advantage to using dendritic-tumor cell hybrids is that it is not dependent on the identification and isolation of specific tumor antigens. Rather, the unaltered tumor antigens derived from the tumor component of the fusion cell is processed and presented to the immune system by the dendritic cell component of the fusion cell. Thus, the immunotherapy approach using dendritic-tumor cell hybrids is applicable to tumors in which these tumor antigens are not well defined, such as SCC. The process of electrofusion should be applicable to all mammalian cells. The fusion of human dendritic cells and melanoma tumor cells has been performed and studied. These fusion cells were able to present antigen to both CD4+ and CD8+ T cells.14 The feasibility of fusing human SCC tumor cells with dendritic cells has also been reported, suggesting that this immunotherapy would be feasible for patients with HNSCC.15
With the use of this treatment model, further analysis can be done on the immune mechanisms responsible for the significant decrease in tumor growth. We are currently investigating cytokine secretion from specific immune T cells obtained by fusion hybrid stimulation. The specific immune T cells will be generated from tumor-draining lymph nodes. These studies will also include immune T cells enriched for CD4 and CD8, since we hypothesize that both cell populations will demonstrate activity from fusion cell stimulation. Efforts are currently being directed in improving this response with other adjuvant treatments. Furthermore, attempts at optimizing the dendritic cell antigen-presenting characteristics via in vitro manipulations during the proliferation dendritic cell culture are ongoing. Analysis of immune cell differentiation due to fusion cell treatment is also being studied. As these mechanisms are further delineated and our understanding of immune responses against SCC improves, treatment of advanced established tumor (ie, 9 days) will be attempted.
In conclusion, these data represent the first preclinical results, to our knowledge, that demonstrate dendritic cell fusion immunotherapy against a murine SCC model and supports using a fusion hybrid vaccine in future human HNSCC clinical trials. Immunotherapy with fused dendritic-tumor cell hybrids can significantly affect 3-day established SCCVII tumor growth. These preclinical data support using fused dendritic-tumor cells as an immunotherapeutic approach against HNSCC.
Correspondence: Walter T. Lee, MD, Head and Neck Institute, 9500 Euclid Ave, Desk A71, Cleveland, OH 44195 (firstname.lastname@example.com).
Submitted for Publication: November 1, 2006; final revision received October 11, 2007; accepted October 14, 2007.
Author Contributions: Dr Lee had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: Lee. Acquisition of data: Lee, Tamai, Cohen, Teker, and Shu. Analysis and interpretation of data: Lee, Tamai, Cohen, and Shu. Drafting of the manuscript: Lee. Critical revision of the manuscript for important intellectual content: Lee, Tamai, Cohen, Teker, and Shu. Obtained funding: Lee and Shu. Administrative, technical, and material support: Lee, Tamai, and Teker. Study supervision: Lee, Cohen, Teker, and Shu.
Financial Disclosure: None reported.
Funding/Support: This research was funded in part by grant 2005-1007 from the Cleveland Clinic Research Programs Council.
Previous Presentation: This research was presented at the Annual Meeting and Research Workshop on the Biology, Prevention, and Treatment of Head and Neck Cancer; August 19, 2006; Chicago, Illinois.
Additional Contributions: Chris Distel, MS, provided technical assistance. Anti-OX40R mAbs were provided by Andrew Wienberg, PhD.


text:
Center and periphery
is a branch of linguistics, the science of language
is composed of two Greek words:
- meaning word, phrase
- denotes learning, a department of knowledge
the literal meaning of the term lexicology is
the science of the word
lexicology is concerned with words, variable word-groups, phraseological units, and with morphemes which make up words
Language subsystems
we regard language as a system of levels - phonic, lexical and grammatical (pronunciation, vocabulary, grammar)
each of the levels constitutes a system in itself
these subsystems are linked and mutually independent
Lexicology and lexicography
the description of the lexical system or lexical components of a language includes lexicology and lexicography
- deals with the meaning and use of words and their mutual relations
- is the practise and theory of compiling dictionaries
Lexicology and lexicography
Two approaches to language study
there are two principal approaches in linguistic science to the study of language material:
the synchronic approach
- is concerned with the vocabulary of a language that as it exists at a given time (for instance, at the present time)
the diachronic approach
- deals with the changes and the development of vocabulary in the course of time
vocabulary = lexis, lexicon
is all the lexical items (words and multi-word expressions) that are used in particular language
the items of vocabulary are organized in a special way (formally or semantically)
vocabulary is the least stable system and has a relatively unlimited number of items in it
here are two principal methods for describing words
one method is by writing a dictionary and the other is by writing a thesaurus
- words are arranged where you can find them (in alphabetical order)
- words that are similar in meaning are grouped together, for example, all the words that are species of fish
- is the base form under which the word is entered and assigned its place; typically the stem or simplest form (singular noun, present/infinitive verb)
Vocabulary as a system
there is a certain lack of balance in the system
the system is in the state of unstable equilibrium with elements in
the periphery
and in
the center
competing each other
there are two opposing forces in the system:
efforts to bring words from periphery to the center
efforts to produce finer distinctions in meaning
the center
- is occupied by lexical units with greatest stability and frequency; in the center prevail notional words (with full lexical meaning) and words with a precisely defined meaning
the periphery
- contains words that are limited in frequency
The extralinguistic reality
is processed in its essential features by human consciousness as a concept (pojem)
the essential features are needed for an effective communication between the users of a particular language
the extralinguistic reality includes real objects, ideas, actions and imaginary reality
between the word and the item of extralinguistic reality (between the form and its content) there is no relationship
words do not name the objects, they only refer to them
the referent of the word
is the object
Word and lexeme
- is a combination of sounds, or its representation in writing, that symbolizes and communicates a meaning
in the early stages of alphabetic writing, letters followed each other in lines without spaces to separate off
spaces between groups of letters became important as the convention of writing evolved
- may be regarded as a sign, an abstract unit, a class of variants
also called lexical item, lexical unit, can means two things:
the word realized by several morphological words (it means that hand and hands are two morphological words, but they are two forms of the same lexical item, which most linguists call a lexeme)
the composite in structure (throw out, stamp collector)
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Lexicology and lexicography
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Kateřina Licháon 10 December 2013
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text:
Apollinaire in the Park 2017
The Visit
by Friedrich Dürrenmatt
July 12-30, Wed.-Sun. 7:30
PORT Park, 99 Marginal, Chelsea, MA
Join us on Stage this summer!
Seeking volunteer performers
for non-speaking roles
in The Visit.
Rehearse at 6:00, perform at 7:30.
Join us for one night or become a regular.
Click here to register!
Early Bird Season Passes
In Honor of Swiss playwright
Friedrich Dürrenmatt this summer we
celebrate all things Swiss!
Your donation will have a huge impact on this production! As huge as particles colliding inside the world's largest and most powerful particle accelerator?
Just maybe!
Chelsea Station restaurant will be on hand
serving fine food and drink!
"If you've never seen a performance at the Apollinaire Theatre Company in Chelsea,
make it
one of your
New Year's resolutions."
-Boston Globe
Outstanding Production
Elliot Norton Award
A Beautiful Day in November on the Banks of the Greatest of the Great Lakes & Stupid F***ing Bird
Thanks to all who came out to celebrate the Opening of the New BlackBox Theater at the Chelsea Theatre Works!


text:
Klan Seeks to Put Float in Georgia Parade
Published: December 6, 1991
ATLANTA, Dec. 5— The Ku Klux Klan wants to enter a float in the annual Christmas parade in Gainesville, Ga., prompting several groups, including four high schools, a church and a Cub Scout den, to pull out of the event.
Officials in the small town on Lake Lanier, about 40 miles northeast of Atlanta, are scheduled to meet on Friday to determine whether the Dec. 14 event should be canceled rather than allow the Ku Klux Klan to enter the float. The title of the float is "I'm Dreaming of a White Christmas."
A dance school was one of the groups that decided not to take part in the parade.
"The majority of the parents said they didn't want their children to participate, so I took that as an indication we should not enter a float," said Nancy Mooney, director of the North Georgia Dance Academy.
The dance school had planned to have a float based on themes from the "The Nutcracker" ballet, Mrs. Mooney said. She said the decision to pull out of the parade was based on concerns for safety of the children rather than unwillingness to participate along with the Klan.
Al Crace, the Gainesville city manager, said officials from Gainesville and Hall County, which jointly sponsor the parade, would consider applications for parade entries on Friday and "decide if there is enough interest to have a parade."
The parade began 11 years ago, he said, and this is the first year the Klan has asked to have a float.
The Klan official who applied for the parade permit, Daniel B. Carver, Grand Dragon of the Invisible Empire Knights of the Ku Klux Klan, was fined $30,000 by a Federal jury for throwing rocks at civil rights marchers in neighboring Forsyth County in 1987.
Mr. Carver could not be reached today for comment.


text:
Alternative Titles
Synonyms: Meguriauyo Baby, A Coincidence, Baby
Japanese: 巡り合うよベイビィ
Type: Manga
Volumes: 1
Chapters: 6
Status: Finished
Published: May 2008 to Nov 2008
Authors: Kinoshita, Keiko (Story & Art)
Serialization: Dear+
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1 indicates a weighted score. Please note that 'Not yet published' titles are excluded.
Ranked: N/A2
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Popularity: #6011
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Visit the
MSc Economics and Econometrics
page on the University of Kent website for more details!
What attracted you to postgraduate study at Kent?When I completed my undergraduate degree at Kent in Financial Economics and Econometrics I felt that there was much more about economics that I wanted to learn and so it made sense to me to pursue a master's degree. The School of Economics is friendly and the staff are very approachable and always willing to offer a helping hand.How are you enjoying the course?I chose the masters degree in Economics and Econometrics because it offered the core topics in Economics as well as offering advanced training in econometrics, which is something that I found that other universities of the same calibre lacked. The teaching at Kent is fantastic for several reasons. All staff are research active so they include this in their teaching, which offers different perspectives on traditional models or theories enabling you to evaluate and critique where necessary. Secondly, classes are small so the environment becomes interactive not only between staff and students but also among students with many debates carrying on after class. The masters students in the School of Economics are very fortunate to have access to their own study room, something that is not usually offered at other universities. This room is equipped with several computers with all necessary software required to complete the degree and a group study area that proved be very useful throughout the academic year.What support do you receive as a postgraduate?The School of Economics is a very supportive department. All academics and administrative staff are friendly and are always willing to help, no matter what the problem is. There is excellent student representation within the School, the School of Economics always asks for the opinion of the student representatives and for the most part carries out their wishes.At Kent there is a Graduate School that offers excellent support to all postgrads and a variety of skills workshops that range from English skills to academic writing and negotiating. The School of Economics has an Economics Society that has arranged guest speakers from business and economics as well as arranging day trips. What do you plan to do next?I plan to go on to pursue a PhD, and then hope to work either for the Debt Management Office here in the UK or in another financial institution.
Why did you choose to study economics at Kent?Initially, I really liked the campus and had heard good things about Kent. I chose economics because I had enjoyed studying the subject and went on to have an excellent undergraduate experience here. When I finished the course, I didn't have a job lined up so thought I would do something productive and carry on with what I knew while, at the same time, improving my career prospects.What have you particularly enjoyed?The School of Economics has felt like a little community, and we all get on well and help each other out. There's a real international mix, too, with students from all over the world. Everyone has a different point of view, so there's always interesting discussions.How have you found the teaching?There is more one-to-one interaction with the lecturers and they really care and get involved, so it feels like we are all working together.How does postgraduate study differ from undergraduate study?It's more intense and there's a lot more work. At undergraduate level you tend to be spoon fed initially then progress to independent working. At Master's level, the lecturers will give you guidance, but it's up to you to read through things to understand the material. So it's the next step up.What's the level of support like?If you don't understand something, you can talk to a member of academic staff after a lecture or there are certain times during the week when they are guaranteed to be in their office. You can just go and ask them – they are very approachable and will take the time to work through a problem with you.
The School of Economics is offering a number of scholarships for outstanding students on MSc programmes for 2016/17.A number of Scholarships for Excellence, worth 60% of the tuition fee for one year, are available for outstanding students, either Home/EU or Overseas.
The School of Economics is offering Alumni Scholarships of £1,000 each, available for undergraduates currently studying at Kent's School of Economics who will graduate in July and continue with an MSc starting in September 2015. These scholarships are in addition to a Kent Graduate School Scholarship.
University of Kent
Find A Masters.
Copyright 2005-2017
All rights reserved.


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Group Description
Join this group to share and get more knowledge about various technical tools, ideas, sofwares and much more available. NO SPAM ALLOWED. If you are promoting a software or website or any other thing and you have a financial or any other intrest in the same, please let everyone know of the same.
All members are encouraged to share their ideas, knowledge about trading using Technical Analysis using any software such as Metastock, Amibroker, or any other. We mainly Analyse the Indian Stock Markets such as National Stock Exchange NSE and Bombay Stock Exchange BSE.
Please note that the Group doesnt encourage posting of Tips / Newsletters, etc. Such posting will be regarded as Spamming and appropriate measures would be taken which may include banning of the concerned member.
Data for Indian Markets is available at
Desi Trader Mall
Group Website
Group Information
- 6130
- Short Term Trading
- Feb 26, 2004
- English
Group Settings
- This is a restricted group.
- Attachments are permitted.
- Members cannot hide email address.
- Listed in Yahoo Groups directory.
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text:
The Chemistry of Swimming Pools
One of the first things I noticed when I bought a pool is that pool chemistry-terms are confusing to both the lay person and to a person with a chemistry background. There is a lot of jargon, which means nothing to a non-chemist, and terms like "alkalinity" and "free chlorine" can be easily misinterpreted by someone who knows their textbook definitions.
1. Chlorine and pH
When pool manufacturers use the term "free chlorine", what they are actually referring to is the chemical hypochlorous acid, HClO, which exists in aqueous solution. This is the actual substance that kills bacteria. By doing so, HClO prevents diarrhea, swimmer's ear (a nasty earache) and various respiratory, skin and infections of minor cuts. Cl2, diatomic chlorine, would also do the trick, but it is more dangerous.
So when you add, say, Ca(ClO)2 to your pool, the product will slowly dissolve in water to produce OCl-(aq).
But this ion creates an equilibrium with water, in other words a pair of reactions that occur at the same rate:
ClO- + H2O = HOCl +
If the pH is too high
the reverse reaction will be encouraged leading to a higher concentration of ClO- , but lowering the amount of essential HOCl. Although a lower pH(more
acid) will discourage the forward reaction (by destroying
Of course, although a
bit of chlorine kills bacteria, too much would irritate eyes, lungs and so
forth. After all it was used as a chemical weapon in
On average such an amount will last two days. But factors such as temperature, use, presence of pool cover(which filters UV) will either increase or decrease HOCl's residence-time.
This is important because trichlor tablets are sold with high levels of cyanuric acid, which is included to drive the HOCl-equilibrium to the right, but unless the owner constantly adds carbonate ion(so-called pH+), the acid will take its toll on pool equipment. For this reason I prefer Ca(ClO)2, but it too has its side effects, as calcium deposits can accumulate, if dilution and backwashes are not routinely performed.
So this is why it is important to constantly monitor pH and chlorine levels in one's swimming pool. Too much chlorine is toxic; too little leads to problems. Too little acid (high pH) will not let HOCl do its job, and too much will be corrosive.
2. Alkalinity
Alkalinity to a chemist means base-level. A base is an acid's counterpart. But to a poolster, alkalinity refers to a specific base, namely hydrogen carbonate ion (HCO3-). The reason it's important to have a decent amount of this in your pool, is that it acts as a buffer and stabilizes the pH level.
3. Measuring pH
The typical pH paper provided in some kits is not at all accurate. It can easily be off by more than 1 unit, and a single unit on the pH scale, a logarithmic one, is really a factor of 10 in terms of H+. Some kits come with phenol red, but it is only useful between pH's of 7 and 8. If the water is more acidic than 7---tap water and rainwater typically are----you will first need bromothymol blue. Take a small sample of tap water and one of pool water. Since the indicator bromothymol blue is dissolved in NaOH, adding four drops to a small vial containing an unbuffered sample will not give the expected green colour for tap water. It will be blue. If your pool water plus four drops of the same indicator is a lighter blue, or greenish blue, it will obviously be more acidic than tap water. It's time add carbonate in proportion to the volume of the pool, and it will correspond to a pH of <6.8 in the chart in the back of the pH+ bottle. The after 12 hours remeasure the pH using phenol red indicator. If it's stillbelow pH= 7.2 add more carbonate. If you accidentally overshot the desired value, it will be time to buy pH(-), and you are on your way to having your own lab.
4. Measuring Chlorine
A common agent used to measure free chlorine is ortho-tolidine, which turns either a light or dark shade of yellow depending on the concentration of HOCl. Ortho-tolidine is unfortunately a cancer suspect agent.
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Technical Asistance: C. Frizzell; and M.Pololos, D.Verrillo and K.Papoulias at EMSB
Comments: email@example.com
Copyright ©2006


text:
Gritty Victorian opener Chris Rogers scored a ton of patience which compels Test selectors to return the favour.
Rogers struck 103 not out for the Bushrangers against South Australia on Monday which will likely save his Test career.
The 37-year-old anchored Victoria's 1-201 in reply to South Australia's 8(dec)-431 on day two of the Sheffield Shield match in Adelaide.
Rogers faced 190 deliveries in his patient knock, which comes as his Test opening berth is under question.
The left-hander's poor recent series against Pakistan returned 88 runs at an average of 22, leading to some critics calling his axing from the Test team.
But Rogers has replied with his only currency - runs - ahead of the four-Test series against India starting on December 4.
And he did it in customary determined style - never flashy, but ever-reliable.
Rogers grafted his 71st first-class century. The most animated he became during the knock was when his opening partner Rob Quiney was given out for 52.
The pair produced a 130-run opening stand which ended when Quiney got a leading edge to an Adam Zampa legspinner.
The ball floated back to Zampa who claimed a low-down catch.
Rogers thought the ball didn't carry to Zampa and repeatedly gesticulated his belief, initially to South Australians and then umpires.
But Rogers' protests were to no avail, yet he soon regained his calm demeanour to blunt SA's bowlers.
Earlier, the Redbacks declared some 20 minutes before lunch with Callum Ferguson top-scoring with 140 and Tim Ludeman making 106, his second first-class ton.
After the centurions were out, Redbacks captain Johan Botha (40 not out), Joe Mennie (36) and Adam Zampa (33 from 12 balls) went on a quest for rapid runs - SA scored 127 runs in 100 minutes in the morning session.
Test quick Peter Siddle was the pick of the Bushrangers' bowlers, taking 2-54 from 23 overs, with Chris Tremain (2-81) and spinner Jon Holland (2-92) other multiple wicket-takers.


text:
Hello everyone,
As part of my MSc Dissertation project at Warwick Business School in UK, I'm conducting a survey that deals with users' experiences of participation in professional virtual communities, of which Spiceworks is perfect example
I would be great if you could help me and participate by clicking on the link below. The survey will take no longer than 10 minutes to answer. All responses will be kept strictly confidential (used only for the purposes of my dissertation). Everyone who participates will have the option to enter into a draw for five £20 (or $30) Amazon vouchers.
Click here to start the survey:
I greatly appreciate your help. My degree depends on this survey! If you would like to know the results of my work, please email me and I will send you the results in September.
Best regards,
Nikita Kursov
23 Replies
Aug 10, 2010 at 8:45 UTC
akp982 is an IT service provider.
Best of luck and hope you get the results you need :-)
You may want to remove your email address from the survey.
Aug 10, 2010 at 8:51 UTC
Intersting survey, I have provided my feedback.
Aug 10, 2010 at 8:54 UTC
How about posting your findings to the community?
Aug 10, 2010 at 9:04 UTC
Thank you for your feedback!
I will be more than happy to post the findings right here, although this will only happen at the end of August.
@akp982: thanks for the tip, but my email address is already quite public due to the survey, so it's not an issue)
Aug 10, 2010 at 9:04 UTC
Done! Good luck. Please post findings if you can.
Aug 10, 2010 at 10:18 UTC
@ KMH: Thank you! I will post the findings right here as soon as there are enough responses and the data is analysed.
Aug 10, 2010 at 10:19 UTC
Done. Hope you get the information you need.
Aug 10, 2010 at 10:33 UTC
Done...good luck with the Dissertation!!!
Aug 10, 2010 at 6:23 UTC
Lawrie Dalman Consulting is an IT service provider.
Completed, very interesting.
Do you plan to publish your results to the community?
Aug 10, 2010 at 6:32 UTC
@Lawrie5252: Thanks for taking the survey! And yes, once complete, I will be posting the results right here at the end of the month!
Aug 10, 2010 at 7:11 UTC
Lawrie Dalman Consulting is an IT service provider.
@Lawrie5252: Thanks for taking the survey! And yes, once complete, I will be posting the results right here at the end of the month!
Thanks Nikita, good luck with the project and we'll look forward to seeing the results when you're done.
Aug 11, 2010 at 3:41 UTC
Done, Good luck :D
Aug 11, 2010 at 6:48 UTC
Good luck with the MSc.
Aug 11, 2010 at 8:02 UTC
Good luck.
Aug 11, 2010 at 10:03 UTC
Done! And good luck!
Aug 11, 2010 at 10:16 UTC
good luck with the Master's, and I agree, I'd be intrested in seeing your results.
Aug 11, 2010 at 3:33 UTC
Many thanks to everyone who took the survey, your help is much appreciated.
Needless to say, I will share all of my findings with the community, but I still need more participants for the results to have any weight, so more responses would be fantastic!
Aug 13, 2010 at 11:56 UTC
Hi everyone, just a quick update:
Firstly, I'd like to thank the 34 Spiceworks members who participated in the survey so far.
Secondly, I plan to close the survey on 20/08/10, soon after which I'll be able to share my results in this thread.
Aug 16, 2010 at 3:32 UTC
Done. I'm looking forward to the results.
Aug 16, 2010 at 6:05 UTC
Niagara Technology Group (NTG) is an IT service provider.
Of course, your results will be skewed by a small percentage of active people in the watercooler being the participants and not a general cross-sampling of the community.
Aug 16, 2010 at 9:14 UTC
Of course, your results will be skewed by a small percentage of active people in the watercooler being the participants and not a general cross-sampling of the community.
Firstly, thanks for taking the survey and secondly, I do agree with your point - admittedly this is a limitation often inherent in quantitative online studies that rely on the kindness of strangers and sometimes fail to capture the views of the less-active participants.
On the positive side, however, I should still have substantial data to make valid conclusions, moreover such limitations are acceptable in the academic world so long as the researchers account for any potential skewness in data analysis and the write-up.
Aug 17, 2010 at 2:24 UTC
Done :) Hope you get some more results!
Aug 17, 2010 at 8:31 UTC
Done. I wish you all the luck with your project.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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Second District Congressional candidate, U.S. Army Reservist and former U.S. Attorney Tim Griffin today issued the following statement to commemorate the signing of the U.S. Constitution on this day in 1787:
"Our Constitution is one of the reasons America is exceptional. As we celebrate its signing, let us pause to remember the patriots who authored the Constitution, those who have defended it and how we can guarantee it is passed on to future generations."
Source: http://www.timgriffinforcongress.com/blog.php


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It seems an absurdly antiquated notion—isn't today's "piracy" limited to music and movies? But political and economic factors have in recent years put international vessels traveling near the coast of Somalia at risk of capture by modern-day buccaneers. Thousands have been held hostage and an unlucky few have suffered torture, murder and use as human shields. As harrowing as it is matter-of-fact, Tobias Lindholm's thriller chronicles a fictive instance of this phenomenon in unerringly realistic terms. A Danish cargo ship traversing the Indian Ocean is overpowered by volatile pirates who appear barely held in check by their multilingual spokesman Omar (Abdihakin Asgar)—whose own agenda is nothing if not ambiguous. As long-distance ransom discussions drag on for months, back in Copenhagen the shipping firm's CEO (Soren Malling) balances conscience with the hardball tactics of a hired professional negotiator (Gary Skholdmose Porter, who has actually performed this function in real life). Meanwhile tempers fray, despair sets in and at least one hostage grows seriously ill.While recent fact-based political thrillers like Argo and Zero Dark Thirty have been praised for their realism, Lindholm's film puts that acclaim in context—those movies seem conventional pop entertainments alongside this gritty, quasi-verite fiction. A Hijacking captures the grueling, dispiriting, claustrophobic tedium of such life-threatening captivity. Yet it's a seldom less than nail-biting exercise in suspense.-Dennis Harvey
Tobias Lindholm
Tobias Lindholm made his feature directorial debut (in collaboration with Michael Noer) on 2010's award-winning R, about a young man's brutal introduction to prison life. An acclaimed screenwriter, he also penned Submarino (2010) and The Hunt (2012) for director Thomas Vinterberg, as well as episodes of the Danish TV series Borgen. A Hijacking marks Lindholm's solo directing bow.


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- high mass
- noun Usage: often capitalized H&M Date: 12th century a mass marked by the singing of prescribed parts by the celebrant and the choir or congregation
New Collegiate Dictionary. 2001.
New Collegiate Dictionary. 2001.
High Mass — may mean: *Solemn Mass, a Tridentine Mass celebrated with deacon and subdeacon (international and general United States [ [http://www.bartleby.com/61/18/M0341800.html The American Heritage Dictionary of the English Language, Fourth Edition, 2000] … Wikipedia
High Mass — High High, a. [Compar. {Higher}; superl. {Highest}.] [OE. high, hegh, hey, heh, AS. he[ a]h, h?h; akin to OS. h?h, OFries. hag, hach, D. hoog, OHG. h?h, G. hoch, Icel. h?r, Sw. h[ o]g, Dan. h[ o]i, Goth. hauhs, and to Icel. haugr mound, G. h[… … The Collaborative International Dictionary of English
High Mass — Mass Mass (m[.a]s), n. [OE. masse, messe, AS. m[ae]sse. LL. missa, from L. mittere, missum, to send, dismiss: cf. F. messe. In the ancient churches, the public services at which the catechumens were permitted to be present were called missa… … The Collaborative International Dictionary of English
High Mass — n. former term for a Mass with parts of the text sung by the celebrant rather than recited and often with added ceremony but without a deacon and subdeacon: cf. SOLEMN (HIGH) MASS … English World dictionary
high mass — high′ mass′ n. rel (often caps.) a mass in which the liturgy is sung, rather than spoken, by the celebrant Compare low mass • Etymology: 1100–50 … From formal English to slang
High Mass — noun count or uncount a religious ceremony in the Roman Catholic Church that is more formal than a usual MASS … Usage of the words and phrases in modern English
High Mass — n [U and C] a very formal church ceremony in the Roman Catholic Church … Dictionary of contemporary English
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High Mass — Rom. Cath. Ch. a Mass celebrated according to the complete rite, in which the liturgy is sung by the celebrant. Cf. Low Mass. [1100 50; ME, late OE] * * * … Universalium


text:
I sit down with Adrian about his films Diffability Hollywood and the upcoming Greetings from Tromaville! We talk about the topics of both films and the struggles of making film and being part of the film industry.
Check out his stuff!
We've got a great interview, today, with Vickey and Bob Beaver from Obatron Productions talking about their latest Kickstarter: Retribution! Made for the Savage Worlds game system, Retribution will take players on a journey into the supernatural where death is not always the end of the story.
We also chat about other projects they have done and the work Vickey is doing, in addition to their fantastic gaming products.
Doc Palindrome stuff:
You can find Vickey and Bob's stuff here:
The long-awaited second episode of my GenCon Film Festival interviews! In this episode, I speak with the incomparable Demetrius Witherspoon and some of the fantastic performers from his epic Submerge series, Erin A. Moore, Shonna Bedford, and Grace Carlton! Enjoy!
Demetrius Witherspoon - Submerge: Ni're Reborn/Submerge: Echo 51
Twitter: https://twitter.com/Dv_E_pictures
YouTube: https://www.youtube.com/channel/UCaauqYkQax-6rNqw_DF52g
Instagram: https://www.instagram.com/submerge_echo51/
Facebook: https://www.facebook.com/SubmergeEcho51/
IndieGoGo: https://www.indiegogo.com/projects/submerge-echo-51-film#/
URL: http://www.dventertainmentpictures.com
Shonna Bedford - Submerge: Echo 51
Twitter: https://twitter.com/shonnabedford
YouTube: https://www.youtube.com/channel/UC37VRfHBfxuGD4NCo8cz4Gw
Facebook: https://www.facebook.com/Shonnabedford/?fref=nf
Instagram: https://www.instagram.com/poetchik1/
URL: http://shonnabedford.wixsite.com/countryblues
Grace Carlton - Submerge: Echo 51
Facebook: https://www.facebook.com/grace.carlton.184?fref=ts
Erin A. Moore - Submerge: Ni're Reborn
Facebook: https://www.facebook.com/emerald.erin.3?fref=ts
So, the first episode, of two, of my trip to GenCon where I mined the Film Festival for some of the greatest interviews I've ever done! Here is the contact information for all the people I spoke with! So many contacts!
Steven Michael Zack - Molly ranger7studios
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URL: http://ranger7studios.com
Note: I may have been thinking of the wrong thing with Ranger 7. That's on me.
Rachel Phelps - Rejoinder Films
Twitter: https://twitter.com/rejoinderfilms
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Richard F. Roszka - All Your Base: Last of Last
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Facebook: https://www.facebook.com/TheSearchForSilverspear/?fref=ts
I have a conversation with Andy about his fantastic project, Merlin of Legend, and talk about his experience in LA and what influences him and what he's into!
Got an extra buck? Support our Patreon!
Twitting: @docpalindrome
Jesse interviews burlesque dancer and model Mary Effen Sunshine! They talk about her career in the adult industry as well as spotlight Mental Health Awareness Month (which they totally rename). Get educated and have fun at the same time!
Check out Mary (Definitely NSFW):
on Facebook!
or Tumblr!
And my Twitter:
Jesse welcomes DocPalindrome.com writer Alize Edeline on the show and they catch up on life and talk about her writing and various obsessions. Old friends catch up and it's all feel-good and wonderful! You'll want to go hug someone after you listen to this!
We welcome Jordan R. T. Spencer, youngest contributor to docpalindrome.com as we talk about his influences, his history, and what he's got coming up!
Surgeon General's Warning: This podcast contains explicit material. More explicit than usual and way funnier, too. If explicit or funny offend you, you should probably avoid this.
Hollywood Weirdo John Karyus, keeper of the oddest IMDB page you will ever see chats with Jess about his career and the life and times of growing up odd.
Sean Sherman is back and joining Jesse to talk with filmmaker Curt Markham about THE MOVIE. We break it down for a while regarding our experience with the past films (while avoiding spoilers), then we dive in for all the spoilery action! Finally, we close up the whole thing with a bit of poetry.
Why not.
Jess sits down with table-top RPG designer Brendan LaSalle to talk about the gaming industry, what Brendan's been up to, and, of course, XCrawl and everything about XCrawl. Oh! And they lament the loss of the greatest MMO of all time: City of Heroes!
Contact: firstname.lastname@example.com
Young writer for docpalindrome.com, Brent Thomas swings by to talk superheroes with Jesse and Sean! Thrill in the Adventure! Experience the Excitement! Enjoy this Episode!
Jesse talks with Cory Pain from Air Wreck Radio, A_Podcast, airwreckradio.com, and all sorts of other cool stuff. They talk about podcasting, comic book movies, and a little bit of politics.
Future Doctor Professor Karl Smith, a.k.a. Karl Sparks of Pocket Radio Theatre, sits down with Jesse and Derek to talk about his upcoming podcast series, The Bootleggers, being a really smart person, and potential apocalyptic scenarios.
Check out his stuff, launching March 15th! poketradiotheatre.org
Jesse Edmond, Joey Organisciak, and Sean Sherman gather, for the first time, to take on geek culture and end up talking tabletop role-playing, super-heroes, and what it means to be a geek!


text:
A large Slideshow of photos taken on the the Music-On-The-Bay Cruise, produced by Ozer Babad.
With Beri Weber, Yitzchok Fuchs, Shua Kessin, Eli Beer and Chaim Lowy.
Beri Weber
Peste Rebbe (Yoeli Lebowitz, Badchen)
Click on the "View Full Screen" button to see the photos larger.
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Awesome Day So Far!
Just enjoying a little bit of quiet time while Clara takes a nap. We are cooking out tonight for dinner! It was a last second decision but I'm excited lol. Had a busy day so far but it's only been the first half. We go one more half to go lol!
!Enjoy your peace, quiet, and cookout!
I love cookouts! Especially when all the family come over. It's a beautiful day here so we're enjoying it too. Have a great rest of the day!
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We had steaks and chops! Plus corn on the cob and baked potatoes. It was delicious!
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BOWLING GREEN _Kent State put itself in position to win a second consecutive Mid-American Conference Tournament title and earn a bid to the NCAA regional tourney by dominating Central Michigan 18-8 at Bowling Green's Warren Steller Field Thursday.
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