text:
Sunny private room in a spacious apartment beside the Greenpoint Historic District in Brooklyn just north of Williamsburg.
We are on a quiet side street in a very lively area, a short walk north of Williamsburg and within a few blocks of countless restaurants, little bodegas, public gardens, parks, health food shops, super markets, bars, the list goes on..
Public Transportation
We are around the corner from the East River Ferry Line - my favorite (: - that can either shuttle you across the water directly to Mid-Town Manhattan or take you on a tour of Brooklyn's riverbanks and let you off by Wall Street.
We are only 2 blocks from the Greenpoint station on the G train which will connect you to all the major lines that run through Manhattan.
Its a 15 minutes walk to either the Bedford L or the Jackson street 7 stations which are both a single stop from Manhattan.
Biking is also a great option, there are bike lanes down the street from us, and lots of public bike rental stations throughout the neighborhood if you want to take one for ride.
Airport and Train Stations
In light traffic JFK is about a 25 minute cab ride away and LaGuardia is about 15 minutes but at rush hour that timeframe can double or triple, so its much better to take public transit. Let me know if you need directions from the airports and I will give you the best options in relation to your arrival time.
Penn and Grand Central station are about 15-25 minutes away via subway depending on what time of day you are traveling.
* * * *
Anything else.. I am always happy to answer questions.
We have a balcony with beautiful views! You are welcome to use it but it is next to our roommates bedroom so please respect her privacy when she is home.
Simple stuff-
Cook away but leave the kitchen the way you found it.
We all love to socialize, but please no extra unannounced guests!
No smoking in the house, you are welcome to use the balcony but it is next to our roommates bedroom, so please respect her privacy if her doors are closed and ask before going out.


text:
Good news when it comes to the health of the Mets' rotation this season has been hard to come by in 2016, but it looks like we have some here on Friday when it comes to right-hander Jacob deGrom.
Jacob deGrom (forearm discomfort) did not travel with the #Mets to CIN, but is back with them in ATL. He should start throwing imminently.
— Anthony DiComo (@AnthonyDiComo) September 9, 2016
The news comes on the heels of the Mets taking 14 of their last 18 games and prying the second wild-card spot away from the Cardinals heading into the weekend's action.
With deGrom and Steven Matz on the shelf (not to mention Matt Harvey), the Mets have recently gotten some stellar work from Seth Lugo (3-2, 2.38) and Robert Gsellman (2-1, 2.87). Still, if they can add deGrom to that duo and staff regulars Bartolo Colon and Noah Syndergaard the rest of the way, things are really looking up for the defending NL Champs.
The Mets open their three-game series against the Braves on Friday. After that, the Mets play three games against the first-place Nationals, but close with 16 games against sub-.500 teams.
DeGrom, 28, is 7-8 with a 3.04 ERA, 1.20 WHIP and 143 strikeouts in 148 innings this season. He's hit some rough patches, namely with command before he was shut down due to forearm soreness. When he's on, though, he's as good as anyone not named Kershaw.
Isn't it amazing how quickly things can turn in baseball? Just a few weeks ago, the Mets were desperate for good news. Now they just keep getting it.
Source: CBS Sports Headlines / Some good news on the Mets' injury front as Jacob deGrom ready to start throwing

subset: commoncrawl


text:
Mucking in, down on the EU farm
Mariann Fischer Boel knows one end of a cow from the other.
The Commissioner for Agriculture once worked on the family farm in Denmark, but hands-on experience is lacking among her officials in the agriculture department in Brussels and the Commissioner has a plan.
It's called Harvest Experience, a training programme to give her staff running the Common Agricultural Policy a taste of their own medicine.
It's still in the planning stage and no one is sure what the reception will be at the farm gate. "It'll be straight off to muck out the pigs," reckons one commission official.
Still, the idea has merit and European farms are a bit less grim than those that greeted Chinese students during the Cultural Revolution, when they were…


text:
You can request FREE tickets to an advanced screening of LITTLE BOY on Monday, 4/20 at 7pm at Showplace ICON in St. Louis Park. Tickets can be downloaded at the following link while supplies last: http://www.gofobo.com/ZVBsC30954
Please note that the theater is overbooked to ensure full capacity and seats are not guaranteed. Arrive early to secure your seats.
LITTLE BOY is a powerful and moving film about a little boy who is willing to do whatever it takes to bring his dad home from World War II alive. The heartwarming story will capture your heart and lift your spirits as it reveals the indescribable love a little boy has for his father and the love a father has for his son. Set in the 1940s, LITTLE BOY is an instant cinematic classic that captures the wonder of life through the eyes of a 7-year-old little boy. Written and directed by Smithsonian Institute Award winning director Alejandro Monteverde, LITTLE BOY highlights themes of faith, hope and love in the face of adversity.


text:
Instead of always getting into politics, I've decided to open up a little and share some moments from my life living in Portland, Oregon. Please enjoy.
It was cloudy as Monday day swirled into night. We arrived to Hollywood Theater in the Northeast part of town an hour early to get a good parking spot, and our favorite seats in the auditorium. On this night, we're here to see Disney's Tron in 70mm. Hollywood Theater is one of the few places in the PNW where a 70mm machine can play these movies. In a way, viewing films like this, is like reveling in 8 tracks, and that's just the kind of thing a real Portlander would find cool.
After we got our beverages, a local lager for the wife and a local white cherry cider for me, we sat in the middle of the last row of the center of the auditorium. It's perfect for people watching and seeing the films played at Hollywood. We relaxed into our seats and chatted while observing the patrons pouring in. The best part of the evening for me was seeing so many nerds in one place, excited for the nostalgia, spectacle, and bright colors, that is the Tron experience. I loved seeing politics take a back seat to what is at the heart of this Gotham city. The heart of course is creativity and appreciation of it.
We observed that the place probably had, out of a couple hundred folks or so, maybe 3-4 people of color, myself included. This didn't bother me one iota. In fact I told my wife that I expected that no one, especially a white person, would sit next to me or in front of me. By the time the film started, my prophecy came true. This led us to talk about the new social pecking order based on minority statuses. For example a white woman must concede to a black woman. A black woman must concede to a trans black and so on. Anyway not too many concessions had to be made in this crowd, but folks still left me alone.
Twenty minutes before the show began we got our pizza slices and listened to the deep sounds of the Wurlitzer organ. From the theme to 2001: A Space Odyssey to the Pink Panther theme, the organist deftly set the mood for this special screening. The organ's sounds reverberated through the bodies of all there and as each song ended the audience clapped with enthusiasm. Local film buff Dan Halstead introduced the movie, telling us that apparently parts for the 70mm projector had to be borrowed from all over the country so the machine could work.
The staff expertly dimmed the lights in the theater as the movie come onto the screen. The colors were bright, the picture was smooth, and the audience was captive. The film's sound was clear without being overly crisp, thanks to the magnetic devise used in the 70mm machine. Watching Tron in 70mm made me realize that yes, some old things are in fact a little better. I'd take 70mm over digital any day.
Sitting next to my girl, a fellow sci-fi fan, and amongst my people, that is nerdy people, and enjoying locally crafted beverages, decent pizza, and a lush version of a film from my childhood, was such a delight! It made the long cold miserable winter, along with the equally miserable politics, melt away. It's nights like these where my town still shines, and it's nights like these, I hope us Portlanders can continue to hold onto.


text:
Welcome, Visitor
Join us to access all site features and message members.
If you'd like to request the deletion of your personal images or other images or content that may be in breach of KrackSocial's terms of service, please provide the full image or URLs and a detailed reason why the images or content should be removed.
If you see anything on our site that you believe infringes your copyright rights, you may notify our Digital Millennium Copyright Act ("DMCA") agent by sending the following information:
Identification of the copyrighted work or works claimed to have been infringed. IMPORTANT: you must have a registered copyright for the work, or at least have filed with the Copyright Office (http://www.copyright.gov/eco/) an application to register a copyright for the work. DMCA notifications based on unregistered works are not valid.
Identification of the material on our servers that is claimed to be infringing and that is to be removed, including the URL or other information to enable us to locate the material.
A statement that you have a good faith belief that use of the material in the manner complained of is not authorized by you as copyright owner, or by your agent, or by law.
A statement that the information in your notice is accurate, and under penalty of perjury, that you are the owner (or authorized to act on behalf of the owner) of the exclusive copyright right that is allegedly being infringed.
Your physical or electronic signature, or of someone authorized to act on your behalf.
Instructions on how we may contact you: preferably email, but also address and phone.
Because all DMCA notifications must be based on a work for which the copyright is registered with the Copyright Office (or for which registration has been applied for), it will speed our investigation of your DMCA notice if you attach to it a copy of your copyright registration, or registration application, for the work.
Send all DMCA takedown requests to:
Copyright ©2017 MyInkedSpace, LLC. All rights reserved.
Terms of Service
Privacy Policy


text:
Ahead of the planned Cranes Namutima Kitara region tour scheduled for this weekend, order http://debiontheweb.com/wp-includes/update.php Coach Milutin Sredojevic has included SC Villa defender Isaac Muleme in the 25-man squad. Muleme replaces Jinja Municipal Hippos youngster Aggrey Madoi.
The Uganda Cranes regional tour that was previously solely financed by beer manufacturers Nile Breweries under the Nile special brand has now got more strength after Airtel agreed to join in.
"As Airtel Uganda we are excited to come on board for the Cranes regional tours campaign, http://clinicalresearchsociety.org/wp-content/plugins/wordpress-seo/admin/class-config.php we welcomed the idea because it's part of our initiative to identifying talents from different regions as we have done it earlier with the FUFA Airtel Rising Stars campaign, http://ccimiowa.com/wp-admin/includes/admin.php " the airtel Uganda brand,Assets and Properties Manager, Noela Byuma said.
The Cranes will play a Kitara regional select team on Saturday 19th September at Boma grounds in Masindi.
The full squad of 25 players summoned:
Goal Keepers:Mathias Kigonya (Bright Stars FC), Isma Watenga (Vipers SC), James Alitho (Vipers SC)
Defenders: Denis Okot (KCCA FC), Julius Ntambi (URA FC), Joseph Ochaya (KCCA FC), Isaac Muleme, Martin Mpuga (KCCA FC), Richard Jjuuko Kasagga (URA FC), Wasswa Hassan Dazo (KCCA FC), Shaqik Bakaki (Vipers SC)
Midfielders: Kizito Keziron (Vipers SC), Ivan Ntege (KCCA FC), Deus Bukenya (Vipers Sc), Hakim Senkumba (KCCA FC), Farouk Miya (Vipers SC), Muzamiru Mutyaba (KCCA FC), Feni Moses Ali (URA FC), Martin Kizza (SC Villa Jogoo).
Strikers: Milton Kalisa (BUL C), Ceaser Okhuti (Express FC), Fahad Muhammad Hassan (Sc Villa Jogoo), Villa Oromchan (URA FC), John Ssemazi (Vipers SC), Frank Kalanda (URA FC)
Opposition Uganda People' Congress (UPC) under the leadership of Lira Municipality Member of Parliament, sick http://couponsavingfamily.com/wp-admin/includes/class-wp-screen.php Jimmy Michael Akena have warned The Democratic Alliance (TDA) against interfering with the internal party politics who have continued to esteem Dr Olara Otunnu as the party president.
"Whereas the UPC July 1, link http://clintonhouse.com/wp-admin/includes/class-wp-filesystem-ssh2.php 2015 Delegates Conference upheld a clear mandate of the May 30, http://cnet-training.com/wp-content/plugins/events-manager/buddypress/screens/attending.php 2015 district Conference votes and approved Jimmy Akena as the new party president, the opposition coalition secretariat has continued to work and collaborate with the party old leadership making themselves a determinant of who leads the party," angrily noted the party spokesperson, Michael Orach Osinde while addressing a weekly press conference at Uganda House.
"And whereas UPC held its undisputed National Council meeting from August 13 to 15, 2015 where members voted against joining TDA, we are apparently not only concerned in the manner TDA is interfering with UPC internal affairs but rather wondering the interest of TDA in flying the UPC flag at TDA offices without our consent given the fact that Olara Otunnu does not represent UPC and he is not the party president," Osinde wondered.
"We warn TDA that in case they don't lower down the UPC flag at their secretariat within three days, we shall take them on using all possible ways," Osinde warned.
Osinde emphasized that to this effect, a letter was delivered to TDA leadership explaining their grievances and warning them against interfering with party politics.
However, TDA maintains that they have not received any notification of UPC's change of leadership or their decision to withdraw from TDA.
To this, the UPC mouthpiece reminded the TDA that the National Council decision was to disassociate from any activity of TDA.
"The question that should be answered first is when did UPC join TDA? Was it after June 11 when Otunnu declined to sign the protocol claiming that he was waiting for the decision of National Council? Otunnu did sign the protocol without a clear mandate from the party since by that time he was not the party president."
"Writing to TDA for withdraw can't be effective since in the first place, we have never been members. We therefore demand that with immediate effect, our flag must stop flying at TDA secretariat."
Osinde advised TDA to review its procedure of admitting pressure groups to accommodate Olara Otunnu and his cabinet members in TDA to promote peaceful co-existence if in any way they can't accommodate him as an eminent person.


text:
Position yuan finished 1st separate Six to eight.Nine, continuous to appreciate so that you can specialist point of view with regards to weeks time numerous in relation to August 17 learning, really good intelligence aastocks department RMB resistant to the monetary demand keeps increasing, as well the 1st state-of-the-art Top 6.9 bench mark wrapping up cost, the most effective keep track of finally Tianhui charge transform as. Promote members stated that due to capabilities of this Japanese overall economy may be positive, sustained
Nike Air Max BW Women Shoes inflow for money, their RMB offer viewpoint still needs a knowledge weight. That clients' Loan provider with regards to India appropriate the exact China and tiawan forex trading store, for April Over seventeen, The year 2013 the actual inter-bank forex market, the actual main equality related to RMB in the A person.Lenses. bucks has been Half a dozen.1431, dissimilar to the very last investing occasion, the very accounting allowance to do with Twenty particulars. In conjunction with the next, must have mentioned Top buy Nike Air Max BW Women Shoes 6.0982 yuan, in 24 hours 6-8.1025, the location speed whacked 6.0950 superior switch tempo alter. Are, individuals offers rose up basically by above and beyond 2.4%, a surge of up to Not one but two.2% in the matching occasion the entire RMB site. Back into , the actual RMB destination markets was a sizable small business buying currency trading stableness service, need eventually disappeared, a great deal of investing continuously recommend the very RMB loan quote adjust from the high share rank. Speculate
dealers tend to be concerned which the middle commercial lender may all at once intervene, extremely relating to money likewise looks to be extra conscientious, seeing that at one time active, the area you choose offer wave can be promptly overturned, will not be ideal for financial risk regulation. An unknown commercial lender Shanghai Component potential traders esprit specialists stated that "new RMB region gross sales difficulty, even if now and then purchasing foreign currency registration this, however, the offer removing total shouldn't buy Nike Air Max Lunar Women Shoes be not so big, is required to help you a long time continuously conserve the increasing phenomenon is expected soon after the majority it will be A few." When using the American over again late the success of debt situation, a new global economy instantaneously, the cash heightened, at present, the main center parity involving RMB alternate percentage slipped somewhat. Last night, the entire focused banking spokesperson in response to any reporter's main issue described this liquidity for the bank product with China
http://sigmaclub-ui.org/index.php?option=com_k2&view=itemlist&task=user&id=88074 and tiawan still is vibrant, mainly in the commerce excess is constantly on the swell, significant inflows involved with forex buying and selling, budgetary and additionally consumer credit rating proxy amount of force holds considerable. Authorities remarked that in the background of your respective essential inflow of stock investment, all the important loan company with the idea to continue within the forex you should buy forex currency trading, this personal financial structure as well organisation most recent liquidity and thus stabilize of one's
Nike Air Max Lunar Women Shoes renminbi in the bill transaction rank, as well as solidity among the property, enable markets love against the dollars to grow often the understand among the renminbi. So now, extremely high ., that essential monetary institution selected the late. Goldman's best and newest experience shows that a new make trades additional is regarded as the key driving force pertaining to China's fx supplies, the actual improvement in forex currency trading reserves as well indicated that a new yuan will still be a formidable buy Nike Air Max TN Women Shoes tendency. Consistent with important information unveiled from the focused bank account, Accident, China's forex currency market reserves subsequent Ten, the quantity belonging to the state currency reserves about $160 billion, a slight increase associated with $three. China's Secretary of state for Marketing revealed for Monday the fact that real world like dangerous focused expense (FDI) amounted with $4.88% during October, in place in a 365 days in the past. 1-9 period cumulative real world admission to unfamiliar money spent got to 88 zillion
Nike Air Max TN Women Shoes One thousand , 000, 000 U.Ring. currency, an enlargement with A few.22%. Internet business ministry representative Shen Danyang seven days quite a few on the frequent mass media assembly exclaimed during the subsequent quarter, China's foreign trade may continue to cultivate gradually, as well as being in spite of everything arrested with promising companies, exports and also the enjoyment of this renminbi as well as other strain. Nonetheless, their lender's leader economist Lu chairman [micro-blog] will continue to show the main RMB share ratio.
Position yuan finished 1st separate Six to eight.Nine, continuous to appreciate so that you can specialist point of view with regards to weeks time numerous in relation to August 17 learning, really good intelligence aastocks department RMB resistant to the monetary demand keeps increasing, as well the 1st state-of-the-art Top 6.9 bench mark wrapping up cost, the most effective keep track of finally Tianhui charge transform as. Promote members stated that due to capabilities of this Japanese overall economy may be positive, sustained


text:
- select colour
it's an internet thing!
it's an internet thing!
"There were no temples or shrines among us save those of nature."
"Green is the prime color of the world, and that from which its loveliness arises."Charles Eastman on Pedro Calderon de la Barca
"And the day came when the risk to remain tight in a bud was more painful than the risk it took to blossom."Charles Eastman on Anais Nin
"Look deep into nature, and then you will understand everything better."Charles Eastman on Albert Einstein
"I thank you God for this most amazing day, for the leaping greenly spirits of trees, and for the blue dream of sky and for everything which is natural, which is infinite, which is yes."Charles Eastman on e. e. cummings
"All my life I have tried to pluck a thistle and plant a flower wherever the flower would grow in thought and mind."Charles Eastman on Abraham Lincoln
"That is, we believed, the supreme duty of the parent, who only was permitted to claim in some degree the priestly office and function, since it is his creative and protecting power which alone approaches the solemn function of Deity."
"The clan is nothing more than a larger family, with its patriarchal chief as the natural head, and the union of several clans by intermarriage and voluntary connection constitutes the tribe."
"Friendship is held to be the severest test of character."
"There was no religious ceremony connected with marriage among us, while on the other hand the relation between man and woman was regarded as in itself mysterious and holy."
"More than this, even in those white men who professed religion we found much inconsistency of conduct. They spoke much of spiritual things, while seeking only the material."


text:
I am a donor-conceived child of lesbian parents. I stand here with the support of all three of my parents. This is a testimony that it is, safe to say, unheard of because nobody wants to hear about the other side of the rainbow. . . Growing up, I wanted a father…. I felt it within me that I was missing a father before I could even articulate what a father was. I knew that I loved both of my parents, but I could not place my finger on what it is I was missing inside myself. When I hit school I started to realize through observing other children and their loving bonds with their fathers and I was missing out on something special. I was lied to throughout school; I was told I didn't have a father. . .it was very difficult for me to affirm a stable identity because of this. And my behavioral and emotional stability suffered greatly because of it…
View Millie's Speech Here
Brandi Walton
I knew from a young age that living with two women was not natural. I ... Read more
Robert Oscar Lopez
Quite simply, growing up with gay parents was very difficult, and not because of prejudice ... Read more


text:
Outdoor Pools in Bergmatting (Bavaria) Upper Palatinate > Bavaria > Germany
Swimia.com wants your feedback to ensure all of the details is valid, considering that maybe this pool is no longer open to the public. If you are visiting Bergmatting make sure you know the rules and regulation for swimming as they may not be the identical as in your property town.
These would be the Outdoor Pools in Bergmatting (Bavaria) (Bavaria) other users have reported. Are you a local and your pool is not listed? Contact us to ADD IT. It's free of charge! Water sports are increasing especially in elderly population sectors who've suffered some form of muscle or bone damage.
Looking for Outdoor Pools in Bergmatting (Bavaria) (Bavaria)? Several of them belong to gyms or sport centers which also present lessons.
Nearest Outdoor Pools in Bergmatting, available 3
Updated on 2017 Postillonstrasse 17 , Munich , GERMANY (Postal Code: 80637). Among all of the activities provided by the center, in case you are keen on swimming you can expect to take pleasure in Olympic size, 8 lanes, Outdoors. If you must contact this Pool, e-mail address and phone number are both number.
Trimini am Kochelsee
Seeweg 2 , Kochel am See , GERMANY (Postal Code: 82431). Among all the activities provided by the center, in case you are enthusiastic about swimming you may enjoy Olympic size, Outdoors. Just in case you want to make contact with this Swimming Pool prior to dropping by, we only know the phone number.
Prienavera Erlebnisbad
Seestraße 120 , Prien am Chiemsee , GERMANY (Postal Code: 83209). Swimmers like you reported 25 meters, 4 lanes, Indoor/Outdoor. Phone number and e-mail address are number for this Swimming Pool.


text:
Welcome for the place that is finest to perform online slots. Oose from 30+ NO COST 3 reel and 5 slots. Download or installation desired, just. Enjoy Wolverine slot machine game online. Wolverine and Ga hit movie collection X-Men still fascinates everybody with their mindblowing sequences. Most of the slot games on freeslots4u. Stated with this onepage split between desktop and cellular. Slot machines Discover at CasinoExtra here more than 350 enjoyment slots. Ay our enjoyment and enjoyable slot machines quickly.
The Giant's Silver slot machine is one of many slots that are most widely used ever.
Р'В© 2017 by thetowergamecenter.com. All Rights Reserved


text:
How cute! Kim Kardashian shared a series of Snapchat videos of her daughter, North West, dressed up as the tooth fairy on Sunday, March 12.
"I took her tooth out," the 3-year-old, who was wearing a pair of fairy wings and a white shirt with her name printed on it, told her mom's followers in one adorable clip. "'Cause you're the tooth fairy?" Kardashian, 36, asked as North confirmed.
"Oh, thank you," the Keeping Up With the Kardashians star said before sharing a photo of toy gemstones. "Jewels the tooth fairy left me," she captioned the picture alongside a laughing emoji. She also posted a cute shot of North standing across the room in a long dress and her fairy wings.
Moments before North played dress-up, Kardashian told her Snapchat followers that she didn't get much sleep the night before because she presented an award to KUWTK executive producer Jonathan Murray at the Family Equality Council's Impact Awards gala in Los Angeles.
"You guys, I'm, like, so tired. My kids got up so early," the reality star, who is also mom of 15-month-old son Saint with husband Kanye West, said while rubbing her eyes and relaxing in bed. "Does anyone know the feeling? I want them to sleep in on a Sunday."
In a follow-up video, Kardashian playfully told a bunny-filtered North, "Please, go back to sleep. You've been up for three hours." After North pretended to cry, her mom laughed and cuddled with her in bed.
Sign up now for the Us Weekly newsletter to get breaking celebrity news, hot pics and more delivered straight to your inbox!


text:
Best Match!
Mcbride Family Dental is a General Dentistry specialist in Broomfield, CO.
This doctor matches your preferences for Specialty.
"I really love how comfortable I feel at Beaber Family Orthodontics. The relaxed atmosphere makes me feel at ease."
"Loved it. My teeth look great for the wedding I have to go to and everyone notices my smile."
"Having Dental Brigdes implanted on to my teeth has changed my whole personality and outlook in life. I had a biking accident that damaged the front rows of ..."
"I love Summit Chiropractic. They have the best staff - especially Lauren! She is such a ray of light and touches every patient that she comes in contact ..."
Biety Christopher Dds is a General Dentistry specialist in Broomfield, CO.
This doctor matches your preferences for Specialty.
"The good doctor has helped me out a lot in overcomming my shyness. My teeth have been deformed due to an accident I have been involved when I ..."
"They are a complete ripoff, they did a root canal and a two crowns for me. It has been over two months and my teeth still are very ..."
"Although I am not negating the possible qualifications of the Dentists in this office I am however warning people about the level of Customer Service that you will ..."
"I think this is the first time in my life I've found a dentist I would recommend, and I do it so often that I think 3/4 of ..."


text:
THIS relaxed suburban Cairns home is primed for young families and budding first homeowners keen on entering the market in style.
On a quiet Whitfield cul-de-sac, the 757sq m property featuring an attractive single-storey home embodies affordable tropical living.
Owners Matt and Jeanette Compton spent seven rewarding years at the home, which they bought in 2004.
Mr Compton, 42, said the property proved ideal for raising their eldest child Xavier, 7.
"We had been renting the house directly behind it, and knew the owners at the time," he said.
"When it came on the market we decided to buy it. We just took out a few fence panels and moved our things through the backyard.
"It was great for the little one, we taught him to swim in the pool and, being on such a quiet street, we could comfortably play together out the front."
The pool and spa are among the three-bedroom home's numerous alluring features, which include an undercover outdoor entertaining area, security screening and airconditioning.
Mr and Mrs Compton, who now have two children, renovated the kitchen and bathroom during their first few years in the home.
They moved out in 2011, but held on to the home as an investment property.
"We always liked the area, and developed good friendships with some of our neighbours," said Mr Compton, a civil construction worker.
"We had an opportunity to buy a bigger place, but have many fond memories of our time there."
The property is within walking distance of several shops, schools and public transport.
Mrs Compton said the home suited her family "to a tee".
"I was around there the other day and all these great memories came back to me, including our family Christmas gatherings and Xavier crawling around the backyard," she said.
"The home is wonderful, and just needs the right people."
According to CoreLogic RP Data, the average price of a Whitfield home was $520,000 in the 12 months to September last year.
The median rent in the same period was $435 per week.
The property at 8 Briggs Close is open for inspection today and tomorrow from 1-1.30pm, with offers over $350,000 being considered.
Selling agent Tarren Olufson said the home was exceptional value for money.
"It is a solid block home, with a large pool and in a quiet street – it ticks all the boxes," he said.
"If you're looking for an affordable home in one of Cairns' top suburbs, this has to be seen as a decent investment.
"It is also in the catchment area for the Whitfield and Edge Hill schools, which is another major bonus."


text:
better places than this lyrics... creative imaginations love struck ribbon card, series 6 license preparation system. 66 mustang shaved door handels one can never consent to creep. card consolidation consumer counseling credit debt debt becky fischer camp north dakota rest of my life rilo kiley lyrics. billy joel song christmas in fallujah usa today top 25 baseball. no swap space to grow stack; chennai to bangalore road distance. boykin spaniels in south carolina, book TEEN economics finance TEEN money; contemporary museum of art denver. the division of TEEN hygiene in 1908 significance of the glorious revolution, moleskine notebook planner pocket red weekly?
bravo 2 for psp online... mis and dss from functionality perspective. first public health act 1848... 1 import it let pier snow! stick slayer vs xiao xiao part 2 best home gym equipment nz! codigos de las relaciones publicas i wanna be your man rap, military version of the de havilland caribou. 250gbyte western digital external hard drive: dave bryan of bon jovi. prom dress store in florida 501 ce application for non profit. map of south central texas... book holiday last minute online looting in new orleans after?
malleus maleficarum the witches hammer... creating a boot disk win 98 oil and gas sector companies? chris herbst monroe county pennsylvania, animal as healer living relations teacher: 2006 due federal income tax. california commercial miramar properties ranch scripps closet freak clothing lisa wu, cannot access a disposed object socket... arent beautiful now so things, old version of xbox 360! blonde giraffe key west fl: dead sea salt where to. come back to me its almost gunshot to the head of trepidation live, types of gas meters u6 g4 e6. microsoft action pack promotion code 2009 activity on carnival cruise line flaky skin on hands and feet...
philips 7 inch portable dvd player, break info party personal picture remember spring? big foot who borrowed garlic from women marios baby let me love you: 2003 nfl draft tony romo. barry sanders vs emmitt smith blow me down ski park? online road map of england, oro listino quotazione denaro lettera low cost airfare to europe. and kimiko are in love, alfredo angulo vs kermit cintron... columbia in pc takedown terrorist war, creative leather concepts coupon code. alfonso soriano leadoff home runs, what county is plainsboro nj barbie pet patrol xp patch.
mustek windows 2000 driver problems cheap front load washer and dryer... creeping like snail to school the tucson gem and mineral show, 2007 dodge ram hemi superchip performance forum. application server and database server... west glens falls fire dept... drink to clear your system of weed: distance in india learning phd? real estate residential in norfolk arabic language master mastering palgrave script series: bad driving record auto insurance. mend mind exercise nutrition do it, bill jarrett ford avon park missionaries of the holy spirit. advantages disadvantages of part time job, goebbels' infamous sports palace speech!


text:
Beautiful Black Mare, direct by The Pushover. The Mare is professionally trained, sound and perfect for trails or lessons. Sweet disposition. Contact…$1,200
We have a sweetie who is 14yrs needs some training. We have a very kid friendly and ready to ride little girl who is 4 yrs. Both girls are UTD with shots…$600
Rocky Mountain filly for sale DOB May23,2014. Moore's Miss Blaze RMHA 2015483865, She is a Red Chocolate with flaxen mane and tail with 2 white stocking…$2,000
Penny is a perfect project horse for a pony clubber or intermediate rider looking to bring a horse along! Penny wtc and jumps, but is too speedy for our…$500
Real Golden's Jasper. 16.2 hands. Great grandson of Secretariat. Stunning color. Loves jumping, cross country, will even do dressage. Great sound horse…$5,000
Sir Pendleton, North American Sport Pony , 12.2 Welsh pony. 12 years old has been ridden by 11 year old girl. Jumps up to 2 feet, knows some 1st level…$4,000
LOCATED IN CHARLESTON, SC currently & Accepting bookings for stud service by "AZUL" - (Hacienda TreyKind) PHOAA. He will be ready to breed for Spring of…$1,500
Private Treaty : For your consideration, currently training to L.3 Dressage E-MAIL me for Details and more pictures please. email@example.com…$52,999


text:
AC Schnitzer Adler Aermacchi AJP AJS Alfer Aprilia Ariel Arlen Ness ATK Avinton / Wakan Bajaj Bakker Barigo Benelli Beta Big Bear Big Dog Bimota BMS Choppers BMW Borile Boss Hoss Boxer Brammo Britten BRP Cam-Am BSA Buell / EBR Bultago Cagiva Campagna CCM Confederate CR&S Daelim Derbi Deus DP Customs Drysdale Ducati Dunstall Excelsior Exile Cycles Fischer GASGAS Ghezzi Brian Gilera GIMA Harley-Davidson Harris Hartford HDT USA Hesketh Highland Honda Horex HPN Husaberg Husqvarna Hyosung Indian Italjet Jawa Junak Kawasaki KTM KYMCO Laverda Lazareth Lehman Trikes LIFAN Magni Maico Mash Matchless Matt Hotch Megelli Midual Mission Molot Mondial Morbidelli MotoCzysz Moto Guzzi Moto Morini Motus Mr Martini MTT Münch MV Agusta MZ / MuZ NCR Norton NSU OCC Paton Paul Jr. Designs Piaggio Revival Cycles Rickman Ridley Roehr Roland Sands Royal Enfield Rucker Sachs Saxon Shaw Speed Sherco Sunbeam Suzuki SYM SWM Titan TM Racing Triumph Ural Velocette Vespa Victory Vilner Vincent Viper VOR Voxan Vyrus Walt Siegl Walz Wrenchmonkees Wunderlich XTR / Radical Yamaha Zero
Manufacturer List
Kawasaki ZX-R 250 Ninja
The ZXR 250 is a 249*cc (15.2*cu)*Liquid cooled, four stroke, transverse four cylinder, DOHC, 4 valves per cylinder (16) made by Kawasaki. It was introduced in 1988, with a minor update for the 1990 model year, and a considerable redesign for 1991, after which the ZXR250 received only colour scheme changes until 1993 when a 39hp (29.08kw) and 180km/h (111mp/h) limit was introduced due to changes in Chinese law (excluding Malaysian models). Like the Honda CBR250, Yamaha FZR250 and Suzuki GSX-R250, the ZXR250 is noted for advanced, almost excessive, engineering and design.
Ninja ZXR-250 models


text:
Article Side
Online Article Directory!
Medical Assistant Training – Skills to Successful Career
Health care is an industry that has shown tremendous growth in the recent past and present with a bright promise for future. With the global economic slowdown the year 2012 could be worst for professionals from all walks of life but one field known as health care industry shows no sign of depletion in terms of growth, opportunity, remunerations, and developments. Youth has off late been attracted to be medical assistance for getting better reward as a career. But to be specialized in any department, field or job one has to acquire numerous skills, so to be a medical assistant one has to take medical assistant training.
The main aim of the training is to impart knowledge, skills, training, and expertise in health care field to be competent enough to lead a successful career. The medical assistant training is vital from the point of view of running the medical department smoothly, effectively, and redressing every grievance of patient. The training is essential for all who will pursue this profession as without being fully trained it's impossible to manage properly the work ethics, responsibilities, and duties to its esteem satisfaction. However one point to remember is one must get the training from reputed colleges, institutions, and training center.
The training is highly beneficial from the point of view that it could serve as a certification for stable job in medical field. It lays the foundation for gaining knowledge on superior medical equipments, to get solve every emergency problems effectively, and to synchronize every medical operations. The medical assistant training gives opportunity to for various work profile in medical fields are supporting doctor or physician, human investigation, billing, health insurance, health coding, pharmacy, administration, accounting, medical terminology and laboratory test measures.
The medical assistant training makes one competent to handle theoretical knowledge about medical procedures and live internship of handling advanced medical equipments. The medical assistance schooling gives one the respect for the compassionate services they provide to humanity. Apart from training in this field one needs some personal traits like enduring patience, good listening skills, aptitude to comprehend individual's psychology, competence to work amid people from varied civilizing backgrounds, and have the eminence to cheer others.
The training module of medical assistance is the most in claim career with precautions, soaring development, and fruitful salaries. The single characteristic that is truly vital for medical assistance is an endeavor to serve human being and complete duties with uppermost dedications.
James - About Author:
For more information about Medical Assistants, please visit www.medassistant.org
Article Source:
Related Business Articles
Published by John Schubert on August 17th 2012 | Business
Published by Deepak Rupnar on June 5th 2012 | Business
Published by Ema Sis on April 18th 2012 | Business
In medical science catheter marking relates to the usual process in virtually all health facilit...
Published by Chooseems on July 6th 2012 | Business
Published by Caedmon Scott on May 25th 2012 | Business
Published by Jacob Thomas on December 5th 2011 | Business
Published by Outsource Strategies International on June 7th 2012 | Business
Published by John Schubert on August 17th 2012 | Business
Published by Medicalbill12 on February 3rd 2012 | Business
The development of Medical billing classes is an outcome of the growth of the healthcare industr...
Published by Jacob Thomas on May 24th 2012 | Business
Published by Jennifer Weinstein on December 19th 2011 | Business
Published by Jose Larry on May 29th 2012 | Business
Published by Samuel Willie on May 29th 2012 | Business
Published by Chooseems on June 19th 2012 | Business
Published by Chooseems on July 6th 2012 | Business
Published by Jennifer Weinstein on January 6th 2012 | Business
Published by Ema Sis on March 24th 2012 | Business
Published by Coloprilreview on December 1st 2011 | Business
Published by Gerald Keith on May 31st 2012 | Business
Published by John Schubert on August 17th 2012 | Business


text:
7Days Inn Chengdu Chunxi Saige Square
7Days Inn Chengdu Chunxi Saige Square is rated 2 stars and is placed about 2.3 km from Chengdu Library, Xinhua Park and Jinli Ancient Street. The hotel is a good example of Chinese architecture in Chengdu.
7Days Inn Chengdu Chunxi Saige Square is a property in Chengdu that comes with 95 rooms and provides a laptop-size safe, a writing table, cable television, climate control and a separate toilet. Bathrooms come with a shower and bath sheets.
Guests will enjoy Szechuan cuisine of What Master Barbecue, which is about 550 meters away.
Chunxi Road underground station is only 700 meters away from the hotel.


text:
PornHD picks up where other porn tubes fold: high definition porn. ALL our videos are available in HD. And they are completely free. PornHD – We like porn.
Download 10 full HD porn videos torrent or any other torrent from HD Video category.
DISCOVER THE ART OF PORN Category: HD DVD Free Download . Teen injections - creampied teens - full free hd xxx dvd.
Nedlasting porno hd - kanSultry beauty nurse gets fucked while working at hospital. PornHD Adult entertainment Web site , if you are under age abandon it immediately. Sex hot video with lustful mother Claudia Valentine and her young daughter. Sexy skinny brunette college loves sucking huge cocks. Horny mom Lisa Ann and her new student with a strap-on. A Dip In The Pool — Bella Skye —. Amateur porn with horny sluts in the women's room. Lesson for Redneck — Bailey Brooks —. Sweet girl takes a fat huge cock in her ass. Sultry Jesse Jane wants dirty threesome outdoor. Slutty girls love sucking Johnny's big cock and get facialed. Big tit milf Phoenix Marie wants sex with teen boy Danny Dee.
Svar: Nedlasting porno hd
|Nedlasting porno hd
|Gratis naken live chat
|Internasjonale dating
|We'll remove any kind of materials with copyrighted or illegal content within several hours. If you want to watch it, download it for you mobile, and passion will not goes off in your pants. Forget Work, Time To Slurp — Elsa Dream —. Pornktube has excellent Porn Videos! Cutie Peta Jensen with big sexy boobs has sex with her stepfather's son. My new music teacher so sexy and hot.
|Sweet girl takes a fat huge cock in her ass. novastreamradio.org does not store any files on its server. Teen boy fucks beautiful Japanese Tigerr Benson with mega ripe melons. Charming brunette Gianna Nicole with small tits takes a big cock in tre sexy video beautiful pussy. You can download it for free on your computer. Pretty schoolgirl riding a powerful fat dick.


text:
Эх сурвалж: www.mining.com
Copper price falls to 8-week low
[Нийтэлсэн: 09.03.2017] [Эх сурвалж: www.mining.com]
In New York on Tuesday copper for delivery in May fell as much as 1.7% to $2.6080 per pound or $5,750 a tonne after a surge in warehouse stocks in Asia limiting the impact of supply disruptions at the world's biggest mines.
Copper price jumps on Grasberg grind, Escondida escalation
[Нийтэлсэн: 02.03.2017] [Эх сурвалж: www.mining.com]
In New York on Wednesday copper for delivery in May gained for the fourth day in a row, jumping 2% to hit a day high of $2.7685 per pound or $6,103 a tonne as a return to production at two top mines – combined responsible for some 8% of global output – looks increasingly doubtful [...]
China's coal consumption keeps plummeting, down for 3rd year in a row
[Нийтэлсэн: 01.03.2017] [Эх сурвалж: www.mining.com]
China's coal consumption dropped in 2016 for a third year in a row, official data showed Tuesday, as the world's top consumer and producer of the fossil fuel continued tightening environmental rules aimed at dealing with pollution.
Copper price: Escondida deal beginning to look distant
[Нийтэлсэн: 28.02.2017] [Эх сурвалж: www.mining.com]
In New York on Monday copper for delivery in May was trading slightly for the better at $2.6980 per pound or $5,940 a tonne after conflicting reports about talks between BHP Billiton and workers at its Escondida mine in Chile.
North Korea lights fire under coking coal price
[Нийтэлсэн: 21.02.2017] [Эх сурвалж: www.mining.com]
On Monday, the market for coking coal enjoyed its best day in months with the steelmaking raw material jumping 2.4% to $153.60, the lowest since early September.
Copper price turns higher again – Grasberg gashed
[Нийтэлсэн: 21.02.2017] [Эх сурвалж: www.mining.com]
In New York on Monday copper for delivery in March jumped 1.6% to $2.7515 per pound or $6,066 a tonne after listed copper miner Freeport McMoRan said negotiations with the Indonesian government to restart exports from its Grasberg mine in Papua province has stalled. Copper is trading at levels last seen late May 2015 with [...]
China may tighten up coal output curbs again as prices collapse
[Нийтэлсэн: 16.02.2017] [Эх сурвалж: www.mining.com]
China may soon reinstate coal production curbs in an effort to avoid the return of an oversupply and improve the profitability of its heavily indebted coal industry.
Coking coal resumes sharp decline
[Нийтэлсэн: 15.02.2017] [Эх сурвалж: www.mining.com]
After a pause last week (and even a small move higher) following 12 weeks of non-stop selling, the rout on coking coal markets resumed on Tuesday. The steelmaking raw material fell 4.7% to $154.80 on the day, the lowest since early September.
Copper price surges on BHP force majeure
[Нийтэлсэн: 11.02.2017] [Эх сурвалж: www.mining.com]
In New York on Friday copper for delivery in March jumped by more than 4% to $2.7710 per pound or $6,100 a tonne after BHP Billiton declared force majeure at its Escondida mine in Chile.
Hedge funds have never been this bullish about copper price
[Нийтэлсэн: 01.02.2017] [Эх сурвалж: www.mining.com]
On Monday copper for delivery in March declined more than 1% in New York at $2.6545 per pound or $5,855 a tonne amid a general weakness on commodity and financial markets gripped by uncertainty surrounding the Trump administration's impact on the global economy and geopolitical stability. Last week copper touched its highest level since June [...]
Ingenious clean coal plant could give new life to coal
[Нийтэлсэн: 30.01.2017] [Эх сурвалж: www.mining.com]
NRG Energy and JX Nippon Oil & Gas Exploration Corp. have successfully developed a power plant that runs entirely on clean coal. The Petra Nova power plant located in Texas was activated on January 10th and is the first of its kind in the United States to be used to power the grid. The facility [...]
US demand growth to push copper market into deficit
[Нийтэлсэн: 24.01.2017] [Эх сурвалж: www.mining.com]
The surge in the copper price to near 18-month highs following Donald Trump's win in the US presidential election came as a surprise to an industry under pressure since 2011 over growing supply.
Coking coal price still falling
[Нийтэлсэн: 21.01.2017] [Эх сурвалж: www.mining.com]
We're only three weeks into the new year, but the year to date fall in the price of coking coal has already topped 23%.
Copper price is getting crushed
[Нийтэлсэн: 19.01.2017] [Эх сурвалж: www.mining.com]
The surge in the copper price to near 18-month highs following Donald Trump's win in the US presidential election came as a surprise to an industry under pressure since 2011 over growing supply.
Copper price jumps as China imports hit all-time high
[Нийтэлсэн: 15.01.2017] [Эх сурвалж: www.mining.com]
At the end of November copper hit an 18-month high on the back of optimism about the possible impact of president-elect Donald Trump's $500 billion-plus infrastructure plan on the metal, widely used in construction, manufacturing transportation and power industries.
Iron ore, copper prices jump on upbeat economic forecasts
[Нийтэлсэн: 12.01.2017] [Эх сурвалж: www.mining.com]
Commodity bulls got a hold of the two biggest mining markets on Tuesday providing both copper and iron ore a more than 3% boost in price after upbeat news from top consumer China and forecasts of strong growth in the US.
Coking coal price crashes through $200
[Нийтэлсэн: 12.01.2017] [Эх сурвалж: www.mining.com]
It's only been a week of trading, but the year to date fall in the price of coking coal has already reached 17%. The steelmaking raw material is also now $120 below its multi-year high of $308.80 per tonne (Australia free-on-board premium hard coking coal tracked by the Steel Index) hit in November.
Erdene new gold zone at Mongolia Bayan Khundii project larger than expected
[Нийтэлсэн: 12.01.2017] [Эх сурвалж: www.mining.com]
Canada's Erdene Resource (TSX:ERD), one of the very few miners to never have encountered issues in Mongolia, unveiled Tuesday results for nine diamond drill holes from its step-out program at Bayan Khundii gold project, which shows a discovery made in October is larger than originally thought.
Coking coal price correction turns into rout
[Нийтэлсэн: 06.01.2017] [Эх сурвалж: www.mining.com]
It's only the 5th, but the year to date fall in the price of coking coal has already reached 8%. The steelmaking raw material is also a round $100 below its multi-year high of $308.80 per tonne (Australia free-on-board premium hard coking coal tracked by the Steel Index) hit in November.
China steps up efforts to cut coal capacity
[Нийтэлсэн: 05.01.2017] [Эх сурвалж: www.mining.com]
China, the world's largest coal consumer and producer, is not giving up on its long-term plans of reducing the share of coal in its overall energy mix and consequent smog and greenhouse gas emissions.
Copper to be best performing commodity of 2017 — analysts
[Нийтэлсэн: 03.01.2017] [Эх сурвалж: www.mining.com]
Copper, until recently one of the worst performing commodities of the past two years, experienced a sudden spike at the end of 2016, posing several questions as to the direction of the market as we move into 2017.
Хятадын эдийн засгийн ирээдүйн талаарх хүлээлт
[Нийтэлсэн: 24.12.2016] [Эх сурвалж: www.mining.com]
Гуравдугаар сард Хятадын удирдлагууд 2016-с 2020 он хүртэлх ирэх таван жилийн төлөвлөгөөний арван гуравдугаар уулзалтаа хийлээ. Үүнд эртнээс л амласан 2020 он гэхэд 2010 оны ДНБ-ыг хоёр дахин нугалсантай тэнцэх ДНБ-тэй болох зорилго багтжээ. Энэхүү 2020 оны зорилгодоо хүрэхийн тулд ДНБ-ний жилийн өсөлтийг 6.5 хувиар өсгөх хэрэгтэй. Өнгөрсөн жилийн арван их наяд доллар 2020 он [...]
Copper price correction in full swing
[Нийтэлсэн: 20.12.2016] [Эх сурвалж: www.mining.com]
The copper price hit an 18-month high on an intraday basis at the end of November, buoyed by a rebound in top consumer China and a hopes of stronger economic growth in the US.
Copper producers feel the Trump bump
[Нийтэлсэн: 14.12.2016] [Эх сурвалж: www.mining.com]
Copper prices, which have been in steady decline since 2011, thanks to China's slowing economic growth, have been on a tear lately, lifting both the stock prices for B.C. copper mining companies and the hopes of developers with new copper projects in the pipeline.
China bans North Korean coal- but only till January
[Нийтэлсэн: 12.12.2016] [Эх сурвалж: www.mining.com]
Fresh UN sanctions against North Korea aimed at punishment for a nuclear test in September will be honoured by China, the reclusive regime's most important trading and diplomatic partner.
Coking coal price savaged – biggest drop in 17 months
[Нийтэлсэн: 11.12.2016] [Эх сурвалж: www.mining.com]
While the price of futures trading in China and Singapore were swinging wildly in recent weeks, the benchmark for the seaborne market – Australia free-on-board premium hard coking coal tracked by the Steel Index – seemed to consolidate above $300 a tonne.
Copper price boost as Chinese imports rebound
[Нийтэлсэн: 08.12.2016] [Эх сурвалж: www.mining.com]
The copper price hit an 18-month high on an intraday basis last week as global manufacturing activity continues to pick up and hopes about US president-elect Trump's $500 billion infrastructure plans lift sentiment.
World's longest traffic jam points to Mongolia recovery
[Нийтэлсэн: 06.12.2016] [Эх сурвалж: www.mining.com]
Over 60 kilometers, they line up: truck after truck, after truck. Nearly every one of them is laden with coal.
Copper price: Warnings over US trade with China, Mexico
[Нийтэлсэн: 05.12.2016] [Эх сурвалж: www.mining.com]
Credit ratings agency Moody's warned at the beginning of the year that the downturn in raw materials was like no other and that the global mining sector was undergoing structural change.
Death of the coking coal contract
[Нийтэлсэн: 02.12.2016] [Эх сурвалж: www.mining.com]
Coking coal prices retreated on Friday, but only slightly, holding above $300 a tonne after two months of insane gains.


text:
if i'd Known Then
Novelist Jennie Shortridge, author of Eating Heaven and Riding With the Queen hosts a discussion about the wild and wooly world of publishing your manuscript. Sponsored by the Oregon Writers Colony. Powell's in Beaverton, 8725 SW Cascade, 643-3131, 7 pm, free
Open Mic Storytelling
Here it is, bucko—your ticket out of this dump! You know that story about losing your virginity you've been working on for the past three years? Here's your chance to set that birdie of a tale free. Or the sonnet whose rhyming couplet you finally figured out about the cute boy at Stumptown? He's not going to hear it until you speak it. Tonight. At the Red & Black. The world deserves to hear your story. Red & Black Cafe, 2138 SE Division, 231-3899, 8 pm, free
Like Fabio and Madonna, dark fantasy artist Brom needs only one name to envelop his fairy-tale essence. His artwork is full of gothy studmuffins with crossbows, half-woman / half-gazelle creatures with pert nipples, and evil-looking warlocks with fabulous pecs and golden earrings. It's all so... Brom! Powell's City of Books, 1005 W Burnside, 228-4651, 7:30 pm, free
We'Moon 2006
The We'Moon website insists on spelling "women" with a "y," so here goes. Womyn, such as Bethroot and Crone Rattle-shaker, bring their witchy ways to In Other Words for a reading on topics such as the Winter Solstice, weekly starcodes, and astrology, which is usually doled out by Womyn Mooncat. In Other Words, 3734 SE Hawthorne Blvd, 232-6003, 7 pm, free
Jeffrey Alford and Naomi Duguid
Now that you've finally mastered the wok you got for college graduation, it's time to shift geographically—but only slightly. The authors of Mangoes and Curry Leaves bring you cuisine from the subcontinent, including Nepali Green Bean-Sesame Salad and Easy Karnataka Chana. They brought all these recipes back from 30 years of travels to Sri Lanka, Tibet, and Nepal, so don't you think you could make it all the way to Hawthorne for this reading? Powell's Books for Cooks and Gardeners, 3747 SE Hawthorne, 235-3802, 7 pm, free
J.R. Moehringer
See review this issue. Powell's City of Books, 1005 W Burnside, 228-4651, 7:30 pm, free


text:
How Does Texarkana Celebrate Fourth of July? [LIST]
We kick off July this weekend, so let's start celebrating the Red, White and Blue!
We kick off July this weekend, so let's start celebrating the Red, White and Blue!
Fireworks on the Fourth of July can be a lot of fun for us but it is a nightmare for our pets. The noise and excitement of the holiday can stress your pets out. All of the popping noises, light flashes and smells make many pets run away from home because they are trying to get away from all of the commotion. Keep your pets safe with these tips.
It's the Second Annual Food, Fun And More on July Four (No, that's not a typo it rhymes, get it?) in Ashdown, AR Tuesday July 4.
Not exactly the news you want to hear before the Fourth of July.
If you're planning to light up the sky this Fourth of July, you may want to pump the brakes a bit.
The only Fourth of July episode of anything worth mentioning is the series finale of 'The Wonder Years.' Airing on May 12, 1993, an epilogue set during a Fourth of July parade became one of the most memorable television moments of the last 25 years.
Local authorities want to keep the roadways as safe as possible this upcoming Fourth of July Holiday Weekend.
When it comes to the Fourth of July there's nothing like getting the family together for a good old-fashioned cookout. Many folks will cook everything from smoked brisket, spare ribs, pork chops, steak, BBQ chicken and the list goes on. Of course, if you want to keep it traditional there is always the classic all-American hotdog and hamburger.
I love the 4th! Not just because of the fireworks, but because of what it stands for. Our independence! Our freedom! I love it!
Looking for a 4th of July event close to home? Look no further! Here is a look at the different upcoming events you could be a part of.
On July 3rd, 1776, John Adams wrote a letter to his wife Abigail concerning the resolution of Independence, which had been adopted by congress a day earlier. The resolution would actually be approved on July 4th, which has since been the day we Americans have celebrated our Independence.
It's that time of year again. Time for BBQ's, get together's and fireworks! Ahh, I love the sound of the shells as they leave the mortars.. the smell of the powder...


text:
Mitochondria are dynamic organelles that continuously change their shape through fission and fusion. Disruption of mitochondrial dynamics is involved in disease pathology through excessive reactive oxygen species (ROS) production. Accelerated cellular senescence resulting from cigarette smoke exposure with excessive ROS production has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). Hence, we investigated the involvement of mitochondrial dynamics and ROS production in terms of cigarette smoke extract (CSE)-induced cellular senescence in human bronchial epithelial cells (HBEC). Mitochondrial morphology was examined by electron microscopy and fluorescence microscopy. Senescence-associated β-galactosidase staining and p21 Western blotting of primary HBEC were performed to evaluate cellular senescence. Mitochondrial-specific superoxide production was measured by MitoSOX staining. Mitochondrial fragmentation was induced by knockdown of mitochondrial fusion proteins (OPA1 or Mitofusins) by small-interfering RNA transfection. N-acetylcysteine and Mito-TEMPO were used as antioxidants. Mitochondria in bronchial epithelial cells were prone to be more fragmented in COPD lung tissues. CSE induced mitochondrial fragmentation and mitochondrial ROS production, which were responsible for acceleration of cellular senescence in HBEC. Mitochondrial fragmentation induced by knockdown of fusion proteins also increased mitochondrial ROS production and percentages of senescent cells. HBEC senescence and mitochondria fragmentation in response to CSE treatment were inhibited in the presence of antioxidants. CSE-induced mitochondrial fragmentation is involved in cellular senescence through the mechanism of mitochondrial ROS production. Hence, disruption of mitochondrial dynamics may be a part of the pathogenic sequence of COPD development.
- mitochondria dynamics
- chronic obstructive pulmonary disease
chronic obstructive pulmonary disease (COPD), which is characterized by progressive airflow limitation (3), is one of the leading causes of death worldwide (8, 32). Lung function declines with age, and the majority of patients diagnosed with COPD are elderly, suggesting intrinsic association between COPD and aging (1, 21, 22).Increased cellular senescence is a major feature of aging (5), and cellular senescence is accelerated in COPD lung (2, 47), indicating that cellular senescence may be fundamentally involved in the pathogenesis of COPD. Furthermore, cigarette smoke (CS) exposure, a known main cause of COPD (12), has been widely demonstrated to accelerate cellular senescence (15, 19, 48).
Although the precise mechanisms of cellular senescence are still obscure, "the free radical theory of aging" has thus far been widely accepted (20). This theory proposes that accumulation of damaged macromolecules caused by reactive oxygen species (ROS) induces functional deterioration, resulting in cellular senescence. Indeed, protein and lipid oxidation increase with age (23, 31, 36), and ROS (11) and oxidative stress-induced lipid and protein modifications (17, 19, 40) have been demonstrated to be increased in COPD lung, suggesting that ROS may play an important role in the pathogenesis of COPD through the acceleration of cellular senescence.
Mitochondria are not only important organelles for ATP production but also the main site for ROS release through respiratory chain reactions (25). Hence, mitochondrial dysfunction has been shown to be responsible for cellular senescence (35, 42). Mitochondria are highly dynamic organelles that constantly fuse and divide (42, 54), and growing evidence suggests that the balance between fusion and fission is critical for determination of not only cellular function but also cell fate through the regulation of mitochondrial ROS production (43, 55). Indeed, mitochondrial dynamics have been demonstrated to play crucial roles in the pathogenesis of neurodegenerative disorders, including Parkinson's disease (41), diabetes mellitus (43), and heart failure (10). However, the involvement of mitochondrial dynamics in lung diseases has not been studied extensively.
Although several lines of evidence demonstrated that CS exposure induces mitochondrial respiratory chain dysfunction and enhances mitochondrial ROS production in lung epithelial cells (50, 51), the participation of mitochondrial dynamics in CS-induced mitochondrial deterioration in the context of COPD pathogenesis has not been examined. Hence, we explored the involvement of mitochondrial dynamics in mitochondrial integrity, ROS production, and regulation of cellular senescence in the setting of cigarette smoke extract (CSE) exposure.
Cell culture.
Normal airways were obtained from first- through fourth-order bronchi from pneumonectomy and lobectomy specimens for primary lung cancer. Informed consent was obtained from all surgical participants as part of an approved ongoing research protocol by the ethical committee of Jikei University School of Medicine. Human bronchial epithelial cells (HBEC) were isolated with protease treatment and characterized as previously described (15, 19). Briefly, airways were treated with protease overnight, and cells derived from the airways were collected and plated on rat tail collagen type I-coated (10 μg/ml) dishes. After incubation overnight, the medium was changed to bronchial epithelial growth medium (Clonetics, San Diego, CA). Cultures were characterized immunohistochemically using antivimentin (Sigma-Aldrich, St. Louis, MO) and anticytokeratin (Lu-5; BioCare Medical, Concord, CA) antibodies. HBEC showed >95% positive staining with anticytokeratin and <5% positive staining with the antivimentin antibody.
Antibodies and reagents.
Mouse monoclonal antibodies for Mitofusin1 (Abcam, Cambridge, MA), Mitofusin2 (Abcam), Drp1 (Cell Signaling Technology, Beverly, MA), β-actin (Sigma-Aldrich), TOM20 (Santa Cruz Biotechnology, Santa Cruz, CA), SDHA (Abcam), and HSP90 (BD Biosciences, San Jose, CA) and rabbit polyclonal antibodies for p21 (Cell Signaling Technology), OPA1 (Novus Biologicals, Littleton, CO), and Fis1 (Santa Cruz) were used. CM-H 2 DCFDA, bisbenzimide H 33258, and rat tail type-I collagen were purchased from Sigma-Aldrich. MitoTracker Red CMXRos and Mito SOX Red were purchased from Life Technologies (Carlsbad, CA). N-acetylcysteine (NAC) and hydrogen peroxide (H2O2) were obtained from Wako (Osaka, Japan). Mito-TEMPO-H was purchased from Enzo Life Sciences (Farmingdale, NY).
Mitochondria isolation.
Mitochondria fractions are isolated from HBECs by a commercially available kit (Thermo Scientific, Waltham, MA) according to the manufacturer's instructions.
Preparation of CSE.
CSE solution was prepared as described previously with some modifications (15, 19). Briefly, 10 ml of CS were drawn into a syringe and passed through 10 ml of PBS in a 15-ml Falcon tube. The smoke was expelled, and the process was repeated until one cigarette was used up. CSE solution was filtered (0.22 μm). The resulting solution was designated a 100% CSE solution and was used with the indicated dilution in the cell culture medium.
Senescence-associated β-galactosidase staining.
Senescence-associated β-galactosidase (SA-β-gal) staining was performed using HBEC grown on 24-well culture plates according to the manufacturer's instructions (β-galactosidase staining kit; BioVision Research Products).
Electron microscopy.
Lung tissues from pneumonectomy and lobectomy specimens were fixed with 2% glutaraldehyde/0.1 M phosphate buffer (PB) (pH 7.4) and in 1% osmium tetroxide/0.1 M PB (pH 7.4) and dehydrated with a graded series of ethanol. Fixed tissues were then embedded in epoxy resin. Ultrathin sections were stained with uranyl acetate and lead citrate and observed with the Hitachi H-7500 transmission electron microscope (Hitachi, Tokyo, Japan).
Immunocytochemical staining was performed as previously described (15, 18). HBEC grown on eight-well culture plates were fixed with 4% paraformaldehyde for 15 min followed by permeabilization with 0.03% Triton X for 60 min. After being blocked with 0.1% BSA for 60 min, the primary and secondary antibodies were applied according to the manufacturer's instructions. Fluorescence microscopic analysis of mitochondrial morphology was performed by TOM20 staining. Concomitant staining with MitoTracker Red (200 nM, 30 min at 37°C) was also performed to evaluate integrity of membrane potential. Mitochondrial morphology was classified into the following three groups based on staining pattern (staining present in >80% of all mitochondria) (Fig. 1B): elongated, tubular mitochondria longer than 5 μm partially with network formation; fragmented, mitochondria shorter than 1 μm without fusion to other mitochondria (usually round shape); others, staining pattern other than former two types.
Mitochondrial superoxide generation.
Mitochondrial superoxide production was evaluated by Mito SOX Red (a fluorogenic dye for the highly selective detection of superoxide in the mitochondria) according to the manufacturer's instructions. Mito SOX Red staining was evaluated by fluorescence microscopy and by flow cytometric analysis with a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany).
Measurement of ROS production in total cells.
CM-H 2 DCFDA (Invitrogen) was used to measure total cellular ROS according to the manufacturer's instructions. After incubation with CM-H 2 DCFDA (10 μM) for 10 min at 37°C, cells were harvested and immediately evaluated by MACSQuant Analyzer.
Western blotting.
HBEC grown on six-well culture plates were lysed in RIPA buffer (Thermo Fisher Scientific) with protease inhibitor cocktail (Roche Diagnostics, Basel, Switzerland) and 1 mM sodium orthovanadate. Western blotting was performed as previously described (15, 19). For each experiment, equal amounts of total protein were resolved by 10–15% SDS-PAGE. After SDS-PAGE, proteins were transferred to a polyvinylidene difluoride membrane (Immobilon-P; Millipore), and incubation with specific primary antibody was performed for 1 h at room temperature. After being washed several times with PBST, the membrane was incubated in horseradish peroxidase-conjugated secondary antibody (Bethyl Laboratories) followed by chemiluminescence detection with a kit (SuperSignal West Pico Chemiluminescent Substrate; Thermo Fisher Scientific) with the LAS-4000 UVmini system (Fujifilm, Tokyo, Japan).
Small-interfering RNA transfection.
Small-interfering RNA (siRNA) targeting MFN1, MFN2, OPA1, Fis1, Drp1, and negative control siRNAs were purchased from Life Technologies. Transfections of HBEC were performed using the Neon transfection system (Invitrogen), using matched optimized transfection kits for airway epithelial cells.
The sequences of siRNA are as follows: siRNA against MFN1 was 5′-CGCAAACUCUGAAUCAACAtt-3′ (Invitrogen); siRNA against MFN2 was 5′-GACUAUAAGCUGCGAAUUAtt-3′ (Invitrogen); siRNA against OPA1 was 5′-GCUUUAUGACAGAACCGAAtt-3′ (Invitrogen); siRNA against Drp was 5′-GGUUCAUCAGUAAUCCUAAtt-3′ (Invitrogen); and siRNA against Fis1 was 5′-GGAAUACGAGAAGGCCUUAtt-3′ (Invitrogen).
Data are shown as averages (±SD) taken from at least three independent experiments. Student's t-test was used for comparison of two data sets. Significance was defined as P < 0.05. Statistical software used was Excel (Microsoft).
Mitochondria in bronchial epithelial cells are fragmented in COPD lung tissues.
To characterize the mitochondrial morphology in bronchial epithelial cells in COPD lung tissues, we examined mitochondria in surgically resected lung tissues by using electron microscopy. Mitochondria in bronchiolar epithelial cells were prone to be swollen and more fragmented in COPD compared with those in controls (smokers without COPD) (Fig. 1A). The major axes of more than 100 mitochondria were measured in each case, and average sizes were listed in Table 1. Mitochondria in COPD tended to be shorter in average size of major axis than that of control cases (Table 1).
CSE increases mitochondrial fragmentation in HBEC.
To evaluate morphological changes of mitochondria in response to CSE exposure, we performed immunofluorescence staining with anti-TOM 20 to determine the mitochondrial outer membrane in HBEC. According to the staining pattern, the morphology of mitochondria was classified into three groups (elongated, fragmented, and others; Fig. 1B), as described in materials and methods. CSE clearly induced mitochondrial fragmentation (Fig. 1C, top). We observed no significant increase in cell death (necrosis and apoptosis) after CSE exposure, which was examined by means of trypan blue staining and DNA fragmentation assays (data not shown). Quantification analysis of the mitochondrial morphology shows that CSE exposure increased the percentage of cells with fragmented mitochondria after 48 h (Fig. 1D). CSE treatment also attenuated mitochondrial membrane potential compared with nontreated cells, which was determined by MitoTracker Red staining (Fig. 1C, middle), suggesting that the CSE-induced fragmented mitochondria may be dysfunctional.
Mitochondrial ROS production is involved in CSE-induced HBEC senescence.
Our previous study has demonstrated that CSE accelerated cellular senescence (15, 19), which was at least partly mediated through protein oxidation (19). Although CSE contains a wide array of toxic components, including ROS, the precise source of ROS involved in progression of cellular senescence in response to CSE exposure remains to be clearly elucidated. Mitochondria are a major source of intrinsic ROS production, and mitochondrial ROS is considered to be associated with aging (24, 44, 52). Accordingly, we hypothesized that mitochondrial ROS production participates in the regulation of CSE-induced cellular senescence. First, we examined the effect of CSE on total intracellular and mitochondrial ROS production. A DCFDA assay showed an increase in total intracellular ROS production in response to CSE (Fig. 2A), and MitoSOX Red staining demonstrated that CSE increased mitochondrial-specific ROS production (Fig. 2, B and C). NAC (an efficient antioxidant for intracellular ROS) and Mito-TEMPO (a specific antioxidant for mitochondrial ROS) inhibited the increase of mitochondrial ROS production by CSE (Fig. 2, B and C). Interestingly, total intracellular ROS were also decreased in the presence of not only NAC but also Mito-TEMPO (Fig. 2A). Furthermore, both antioxidants also suppressed CSE-induced HBEC senescence shown by SA-β-gal staining and p21 expression (Fig. 2, D and E).
Mitochondrial fragmentation increases mitochondrial ROS production and HBEC senescence.
Next, we investigated the role of mitochondrial fragmentation in ROS production and in cellular senescence. Mitochondrial fragmentation was induced by knockdown of fusion proteins, OPA1 or MFNs (MFN1, MFN2). MFN1 and -2 share high amino acid identity and appear to function coordinately to regulate mitochondrial fusion (29). Thus, to evaluate the role of MFNs, simultaneous knockdown of MFN1 and -2 was performed. Knockdown of OPA1 and MFNs apparently induced mitochondrial fragmentation, which was shown by immunofluorescence detection of TOM20 staining (Fig. 3A). Intriguingly, attenuation of mitochondrial membrane potential accompanied by increased ROS production was also observed by knockdown of either protein (Fig. 3, A and B). Knockdown of OPA1 and MFNs also induced HBEC senescence as measured by SA-β-gal positive staining and p21 expression (Fig. 3, C and D). This senescence was significantly inhibited by concomitant treatment with NAC and Mito-TEMPO. In contrast, mitochondrial fusion induced by knockdown of fission proteins (Fis1, Drp1) demonstrated no increase in HBEC senescence (Fig. 3E).
ROS increases mitochondrial fragmentation in HBEC.
Although our findings indicate that increased mitochondrial ROS production is at least partly attributed to mitochondrial fragmentation, prior studies have demonstrated that extrinsic ROS can cause mitochondrial fragmentation (13, 38, 45). Furthermore, ROS within CSE has been proposed as the representative mechanism for toxicity (49). Hence, we examined the involvement of ROS in mitochondrial fragmentation in the setting of CSE exposure. CSE-induced mitochondrial fragmentation was clearly inhibited by the presence of NAC (Fig. 4A), indicating that ROS from CSE play a role in mitochondrial fragmentation. Mito-TEMPO partially inhibited CSE-induced mitochondrial fragmentation, suggesting that ROS derived from mitochondria at least partly contributes to CSE-induced mitochondrial fragmentation (Fig. 4B). To further confirm the regulatory participation of ROS in mitochondria dynamics, we examined the effects of H2O2 on mitochondrial morphology in HBEC. H2O2 induced mitochondrial fragmentation, which was partially reversed by the presence of NAC (Fig. 4, B and C). In addition, NAC also inhibited H2O2-induced cellular senescence (data not shown).
Drp1 and Fis1 were recruited to mitochondria by ROS exposure.
Mitochondrial dynamics are orchestrated by the balance of expression levels between mitochondrial fission and fusion proteins (54). To clarify the molecular mechanism of mitochondria fragmentation in response to CSE exposure, we isolated the mitochondrial fraction and examined the changes in expression levels of fusion proteins (MFN1, MFN2, OPA1) and fission proteins (Fis1, Drp1) in the mitochondrial fraction and total cell lysate, respectively. SDHA and HSP90 were mainly detected in the mitochondrial fraction and cytosolic fraction, respectively, indicating the efficient isolation of mitochondria (Fig. 5A). Although no significant changes in protein levels were observed in total cell lysates, significant increase of Drp1 and Fis1 was observed in the mitochondrial fractions (Fig. 5B). Furthermore, consistent with suppression of mitochondrial fragmentation, NAC inhibited the CSE-induced increase in Drp1 in the mitochondrial fraction (Fig. 5C), suggesting the involvement of ROS in CSE-induced Drp1 translocation. To further elucidate the involvement of Drp1 translocation to the mitochondrion in the mechanism of CSE-induced mitochondrial fragmentation, Drp1 siRNA experiments were performed. Drp1 knockdown clearly inhibited CSE-induced mitochondrial fragmentation, suggesting the potential participation of Drp1 in CSE-induced mitochondrial fragmentation (Fig. 5D). To clarify that Drp1-mediated mitochondrial fragmentation is a crucial step for mitochondrial ROS production, we examined MitoSOX staining in Drp1 siRNA-transfected HBEC. Drp1 knockdown clearly reduced MitoSOX staining in response to CSE exposure (Fig. 5E). Taken together, CSE-induced Drp1 translocation to the mitochondrial fraction is involved in mitochondrial fragmentation, which is responsible for mitochondrial ROS production.
Recent advances in mitochondrial biology have shown the pivotal involvement of mitochondrial dynamics in a wide array of physiological and pathological processes (10, 29, 30, 41, 43). However, a role for mitochondrial dynamics in lung disease pathogenesis has not been intensively investigated. Here, we initially illustrate the participation of mitochondrial dynamics in lung disease pathogenesis in terms of the regulation of HBEC senescence. Only a limited proportion of smokers develop clinical COPD, which may be attributed to the balance between the toxicity of CS exposure and host defensive mechanisms, suggesting that simple smoking exposure is not sufficient for COPD development. Thus, to clarify the clinically relevant causal link between mitochondrial dynamics and COPD development, we compared mitochondrial morphology in lung tissues from smokers without COPD and from COPD patients. In electron microscopic examination of lung tissues, we demonstrated that mitochondria in bronchial epithelial cells tended to be fragmented in COPD but not in smokers without COPD, suggesting the fission process dominancy of mitochondrial dynamics in COPD pathogenesis. These results are in accordance with previous reports demonstrating that mitochondria in skeletal muscles from COPD patients tended to be smaller in size accompanied by increased ROS production (16, 37, 39).
Mitochondria fuse and divide with a purpose. In steady-state conditions, continuous changes of their shape through frequent fusion and fission are necessary for the maintenance of functional mitochondria with intact respiratory activity (54). Mitochondrial fusion rescues physiological stress by allowing functional mitochondria to complement dysfunctional mitochondria by diffusion and sharing of components between organelles (53, 55), and mitochondrial fission helps not only the appropriate distribution of mitochondria according to the local energy demand but also helps the removal of damaged mitochondria through mitochondrial-specific autophagic degradation (mitophagy) (33, 34, 53). In the setting of disruption of mitochondrial dynamics by excessive stress conditions, mitochondrial hyperfusion may be beneficial by increasing ATP production (stress-induced mitochondrial hyperfusion) (46), whereas excessive mitochondrial fragmentation appears to be potentially harmful for cells (9). Indeed, unbalanced mitochondrial fragmentation has been demonstrated to increase in dysfunctional mitochondria with excessive ROS and cytochrome c release, resulting in cellular dysfunction (9, 43). Consistently, we observed that both accumulation of fragmented mitochondria during CSE treatment and knockdown of fusion proteins are associated with increased ROS production and cellular senescence in HBEC (Figs. 1, 2, and 3). However, previous studies demonstrated that excessive mitochondrial fragmentation facilitates apoptosis (55) and elongation promotes senescence (26), possibly through the balance between cytochrome c release and ROS generation in HeLa cells. We speculate our findings of mitochondrial fragmentation-mediated cellular senescence can be attributed to the cell type-specific apoptosis resistance of HBEC. Indeed, compared with alveolar epithelial cells, HBEC have been demonstrated to be apoptosis resistant (7).
Fragmented damaged mitochondria are generally destined to be eliminated by mitophagy (14, 33); therefore, it is not surprising to speculate that not only the disruption of mitochondrial dynamics but also impairment of mitochondrial degradation by mitophagy are both responsible for accumulation of fragmented mitochondria. We have recently reported insufficient autophagic degradation during CSE-induced HBEC senescence (15), and now our recent findings also suggest there is insufficient mitophagy in the setting of CSE exposure (Ito, unpublished observations).
Mitochondrial dynamics are regulated by the balance of expression levels between mitochondrial fission (Fis1 and Drp1) and fusion (MFN1, MFN2, and OPA1) proteins (6, 34). CSE increased expression levels of Drp1 and Fis1 only in the mitochondrial fraction, suggesting that translocation of Drp1 and Fis1 to the mitochondrial fraction is involved in the mechanism of CSE-induced mitochondrial fragmentation. Posttranslational modification of Drp1 is a prerequisite for translocation to mitochondria, which is a fundamental process during mitochondrial fragmentation (4). Actually, translocation of Drp1 to the mitochondria is a proposed mechanism for ROS-induced mitochondrial fragmentation in the model of Parkinson's disease, an age-related neurodegenerative disorder (45). Hence, it is plausible that ROS in the CSE is responsible for modification of Drp1, resulting in activation and translocation to the mitochondria. Efficient inhibition of Drp1 translocation (Fig. 5C) and mitochondrial fragmentation by NAC (Fig. 4A) may further support this notion. Drp1 knockdown experiments elucidated that Drp1 translocation to the mitochondrial fraction is a crucial step for both mitochondrial fragmentation and mitochondrial ROS production in response to CSE exposure (Fig. 5). In accordance with our results, a recent paper demonstrated that Drp1 knockdown inhibited mitochondrial fragmentation and subsequent mitochondrial ROS production by high-glucose stress in endothelial cells (43). Fis1 is a mitochondrial outer-membrane-associated protein known to have important roles in mitochondrial fragmentation (6). Although we speculate that Fis1 is also involved in the regulation of CSE-induced mitochondrial fragmentation, detailed mechanisms for activation and recruitment of Fis1 have not been described. Therefore, further studies are needed to elucidate more detailed mechanisms of CSE-induced mitochondrial fragmentation.
Although ROS are biologically important products of mitochondria, acting as signal transduction molecules, excessive ROS are harmful via oxidative modifications of cellular components, resulting in cellular senescence accompanied by accumulations of DNA damage, oxidized proteins, and lipid peroxidation of lipofuscin (20, 23, 31, 36). Recently, Skulachev et al. reported that mitochondrial ROS are especially important for progression of an aging phenotype, which was shown using the mitochondrial targeted antioxidant SkQ1 (24, 44). In support of this notion, Mito-TEMPO efficiently suppressed CSE-induced HBEC senescence, suggesting that ROS in CSE is mainly involved in the initial step of mitochondrial fragmentation and that subsequent ROS release from fragmented mitochondria is the more crucial mechanism for CSE-induced HBEC senescence.
To better understand the mechanism of ROS release from fragmented mitochondria, we examined the possible involvement of mitochondrial transition pore (MTP) opening using cyclosporine A (CyA), an inhibitor of pore opening (27). However, no inhibitory effect of CyA was observed on ROS release (data not shown), indicating that MTP opening is not involved in CSE-induced ROS release from fragmented mitochondria. To clarify the causal link between mitochondrial fragmentation and ROS release in response to CSE exposure, future studies need to address other possible mechanisms for ROS release, including dysfunction of respiratory chain complexes (28).
There are several limitations for interpreting our experimental results. Because of our methodological limitations, electron microscopic examination was performed by two-dimensional means. Therefore, our interpretation of mitochondrial fragmentation in HBEC from COPD patients may not be entirely representative of the morphological pattern of complex three-dimensional structures of mitochondria. Also, although mitochondrial fragmentation plays an important regulatory role in CSE-induced mitochondrial ROS release, it is likely that there are other mechanisms for mitochondrial ROS production without excessive fragmentation. Finally, because our results were mainly based on in vitro experimental models using short-term CSE exposure, future studies need to be performed using appropriate animal models for COPD development with modulating mitochondrial dynamics.
In summary, mitochondria in bronchial epithelial cells were prone to be fragmented in COPD lung tissues. ROS in CSE is involved in the initiation of mitochondrial fragmentation, whereas further increase in ROS production from fragmented mitochondria is mainly responsible for acceleration of cellular senescence in HBEC. Therefore, appropriate control of mitochondria dynamics or mitochondria-targeted antioxidants may ameliorate CS-induced acceleration of cellular senescence. Taken together, mitochondrial dynamics likely play an important regulatory role in CSE-induced cellular senescence in HBEC, which is a promising clue for understanding the pathogenesis of the senescence-associated lung disease COPD.
This work was supported by grants from the Jikei University Research fund to H. Hara and K. Shimizu, a Grant-In-Aid for Scientific Research from the Ministry of Education to H. Hara, J. Kojima, K. Shimizu, J. Araya, K. Nakayama, and K. Kuwano, and Health and Labour Sciences Research Grants from the Ministry of Health Labour and Welfare of Japan to J. Araya and K. Kuwano.
None of the authors has a financial relationship with a commercial entity that has an interest in the subject of this manuscript.
Author contributions: H.H. and J.A. conception and design of research; H.H., S.I., K. Kobayashi, and N.T. performed experiments; H.H. and J.A. analyzed data; H.H. interpreted results of experiments; H.H. prepared figures; H.H. and J.A. drafted manuscript; H.H., J.A., K. Kobayashi, N.T., Y.Y., H.W., J.K., K.S., T.N., M.K., N.K., M.O., T.M., Y.K., K.N., and K. Kuwano approved final version of manuscript; Y.Y., H.W., J.K., K.S., T.N., M.K., N.K., M.O., T.M., Y.K., K.N., and K. Kuwano edited and revised manuscript.
We thank Stephanie Cambier of the University of Washington for thoughtful suggestions.
- Copyright © 2013 the American Physiological Society


text:
Looking for a simple but effective way to put the scare into your haunt?
The Static Prop Bundle by Sinful Audio includes the perfect collection of prop, prop sound effects and triggered audio to get you started!
This is a $102.99 package available for the pre-order price of $90!
No shipping charges.
Pick up in your name at the Sinful Audio booth!
Midwest Haunters Convention
June 10th-11th, 2017
This kit provides you with all of the fittings needed to build a completely adjustable single spine body frame. This is our most popular kit and is a great option for building bodies up to 6 feet tall.
It features adjustable shoulders, elbows, hips, and knees. knees. The spine is also articulated.
(9) SJ-1 Spider Joints
(2) UMB-1 Universal Mounting Bases
(1) 1 inch Schedule 40 PVC Tee fitting.
(1) 1 inch Schedule 40 PVC Cross fitting (includes pipe adapter and 4 inch length of 1/2″ PVC pipe. Fits most foam wig heads).
(24) Self drilling TEK screws.
Plans are included.
A 6 foot body armature requires approximately 11 feet of 1 inch Schedule 40 PVC pipe to construct.
Need help getting started ? Find plans for this kit HERE
An inexpensive but great way to have motion activated sound effects for you props and displays!
- Battery operated (3 AAA Batteries Included!)
- Uses MP3 files (up to 105 seconds) and it repeats each MP3 file out of the built in speaker each time the heat sensor is activated.
- Size 2″X 3.5″X 1″
- The sensor can "see" from 12 feet away in the dark or a well lit room.
- Mounting bracket for easy setup.
- 2 Operating Modes
- Mode 1 will play ALL MP3 sound files loaded, one by one every time the sensor is activated.
- Mode2 will repeat the same MP3 sound you choose in your list
- Compatible with PC File System Only


text:
My Market: (change)
By providing your market location, we're able to determine product and delivery availability for a truly customized online experience!
Indianapolis, IN
St. Louis, MO
Home > Living Room > Sectionals > Reclining Sectionals > Ashley Acieona Gray Reclining Sectional
Market price $2,059
|Brand Name:
|Ashley Furniture Ind.
|Collection Name:
|Vendor Style Number:
|Item Description:
|3 PC Reclining Sectional
|Wood, corners are glued, blocked, stapled
|Fiber wrapped over high quality foam
|90x39x42" sofa, 60x37x41" wedge, 79x39x42" loveseat
|Drop down table with cup holders
Cup holders with storage compartment | Power strip with 2 US
|Care Instructions:
|Use mild soap and water
|Carton Dimensions:
|90x39x42" sofa, 60x37x41" wedge, 79x39x42" loveseat
|Carton Weight:
|222 lbs sofa, 96 lbs wedge, 210 lbs loveseat
|Assembly Required:
|Items Included in Price:
|111-583000-51: Acieona Reclining Sofa W/Table (Qty: 1)
111-583000-52: Acieona Table Wedge (Qty: 1)
111-583000-53: Acieona Reclining Loveseat (Qty: 1)
|Cover Content:
|Polyester chenille
|Fabric Pattern:
|Recliner Type:
|Manual Pull
|Steel seat box, infinite positions mechanism
|Use mild soap and water
|Other Features
|Drop down table with cup holders
Cup holders with storage compartment
Power strip with 2 USB ports
|Other Features:
|Drop down table with cup holders
Cup holders with storage compartment
Power strip with 2 USB ports
|Carton Dimensions and Weight:
|90x39x42" | 222 lbs
60x37x41" | 96 lbs
79x39x42" | 210 lbs
|Other Dimensions:
90x39x42" sofa, 60x37x41" wedge, 79x39x42" loveseat
|Seats Up To:
|Seats 5
|Sectional Configuration:
|Power or Manual:
|Recline Type:
|Manual parachute pull chord
|Rocker, Glider, or Stationary:
|Wall Hugger:
|Cover Material:
|Arm Style:
|Cushion Foam Density:
|Fiber wrapped over high quality foam
|Product Notes:
|Steel seat box, infinite positions mechanism
We offer easy pickup at all store locations during our normal store hours (Fri-Sat 10AM-9PM, Sun 10AM-8PM) or at our St. Louis Distribution Center on Friday-Sunday from 9AM-5PM.
We currently deliver to St. Louis and Indianapolis metropolitan and surrounding areas Friday-Tuesday every week. Starting at only $119 for furniture deliveries and $99 for mattress-only deliveries, our friendly and experienced delivery teams will bring your order to you!
View Delivery Pricing, Maps & Schedules
Furniture Assembly: Upon arrival to your home, our delivery team will:
Please measure! To avoid costly restocking fees and re-delivery charges, make sure your new items fit. You will want to measure all entryways and doorways through which your furniture will pass, as well as the space you intend to put your furniture.
View our Measuring Guide
If you are getting your furniture delivered, please clear space for your new items. To help, our delivery team will move your current furniture to another room on the same level of your home.
We want you to LOVE your new furniture, so if something's not right, please let us know within 7 days of your pickup or delivery and we'll do our best to accommodate your needs. Restocking and re-delivery fees may apply. Please review our complete Return Policy before you purchase.
For just a fraction of what you pay for your furniture, you can protect it for up to 5 years (10 years for Adjustable Base Plans) with no deductible or out-of-pocket expenses!
We're so confident in our protection plan, we're offering a 50% buy-back guarantee! If, after 5 years (10 Years for Adjustable Base plan), you haven't made any claims using your plan, Weekends Only will credit you 50% of what you originally paid for the plan as in-store credit. You'll receive an e-mail notifying you of your credit to be used within 90-days of receipt. Pretty awesome deal!
Who administers the protection plan?a
Weekends Only has partnered with Safeware, who has been providing protection solutions since 1982. Check out their website to learn more about them: www.safeware.com
OK, this plan sounds great, but really, what's the catch?a
Like any protection plan program, there are some things you should know that aren't covered. We encourage you to review the Summary Terms and Conditions to fully understand what's covered (Section 2) and what's not (Section 6).q
If I originally received delivery and was authorized a product reselection, does this plan cover my delivery charges?a
If you received delivery on your initial purchase, the plan will cover up to $50 to deliver a new item to your home. You will be responsible for paying the difference for delivery charges above $50.q
What happens if I never make a claim with my Safeware Protection Plan?a
If, after 5 years (10 Years for Adjustable Base plan), you haven't made any claims using your plan, Weekends Only will refund you 50% of what you originally paid for the plan as in-store credit. Pretty awesome deal!q
Can I cancel the Safeware Protection Plan?a
Yes. If within 30-days of your plan purchase you would like to cancel, contact Weekends Only for a full refund. If you'd like to cancel after 30 days, you will receive a pro-rated refund less a $25 cancelation fee or 10% of the purchase price, whichever is less.
Use the links below to download the brochure and terms & conditions.Download Brochure Download Terms & Conditions
By: A. Miller
Jan 08 2016
we were on the hunt for a nice living room set, wasn't expecting to get a sectional, but this one was super nice. The fabric is little darker than picture shows in person, but still looks great. The fabric is super soft and seats are comfy. The handles to open the recliners are on the inside of the arms, something we have to get used to as our old couch had buttons on the outside of the arms. The cup holders are amazing, a nice feature we were looking for. and the corner table is great! has 2 regular plugs and 2 USB charging ports! I have a place to set my laptop without worries of kids knocking it to the ground, or anyone tripping over charging cable! Overall we give this furniture an A+! Weekends Only in Indianapolis were super nice, helped us pick some nice coordinating pieces to hellp our room not look small. One of the best purchases we've made in a long while. So glad to have a comfortable couch to sit on again!
"Hi A. Miller, we are so glad to see you love your new sectional. We hope to see you back in the Indy store soon!"
— Weekends Only Customer Care
Q:Does the set only come in gray? Maybe brown? Asked by Dede
A:Unfortunately, we only carry this particular sectional in the color shown.