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User:Kaila T Bryant/Notebook/Biology 210 at AU
March 23, 2016 Purpose: To see the negative and positive effects of Amoxicillin and Metoprolol on the embryos of zebra fish.
Materials and Methods:
Day 1: Using Zebra fish water, fresh water pre-prepared with an anti fungal, methylene blue, to help them survive; Metoprolol was mixed into the 500 ml flask with the water at a concentration of 50 mg/ml. (10) This did not dissolve, so the mixture was heated at over 150 degrees Celsius and mixed using a magnetic stir bar. Although this did create some break down, it did not fully dissipate the drug. Following this, 6 ml of Zebra fish water, in a fresh 500 ml flask, was mixed with one tablet of Amoxicillin at 858 mg a tab. This putting the mixture at about 140 mg/ml which is what the paper used as reference instructed (9). After being magnetically stirred Amoxicillin seemed more soluble, so it was left alone. After letting the mixtures cool, the control, regular Zebra fish water, and both of the mixtures were placed into 24 well dish. After selection 20 healthy embryos, a dropper was used to transfer the egg in the each well.
Day 2-3: The fish were left to develop and grow in the lab, they were not fed nor jostled in this time period.
Day 4: A standard check up on the fish was taken and care was taken to replace any low water wells. The fish were examined under a dissection microscope to check for any death. The remaining fish were fed 15 microliters of brine shrimp with a P1000 pipette.
Day 5: Standard check up on fish was taken. Care was taken to replace any low water wells and take out any old empty egg cases. Each fish was observed under a dissection microscope to ensure it was living. 10mLs of solution was removed from each well and dependent upon the condition, 25 mLs of fresh water was added corresponding to the letter on the well. The remaining fish were again fed 15 microliters of brine shrimp with a P1000 pipette.
Day 6: The same steps as day 4 were taken.
Day 7: Standard check up on fish was taken. Care was taken to replace any low water wells. Each fish was observed under a dissection microscope to ensure it was living. 5mLs of solution was removed from each well and dependent upon the condition, 5 mLs of fresh water was added corresponding to the letter on the well. 3 embryos were selected to fix and put in 2% tricaine. After they were put in formaldehyde to preserve their bodies for measurement and observations later. Two fish from each group, A, B and C, were taken and put into a slide with water from their well and observed under a compound microscope. These fish were assessed on different levels: movement, speed, movement after being jostled or prodded, eye movement, heart beat, stage of development, curve of tail, swim bladder, fin size and pigmentation. They were then placed back into their wells using disposable pipettes. The remaining fish were again fed 15 microliters of brine shrimp with a P1000 pipette.
Day 8: The same steps as Day 6 were taken.
Day 9 - 10: The fish were left to develop and grow in the lab, they were not fed nor jostled in this time period.
Day 11: Standard check up on fish was taken. Each fish was observed under a dissection microscope to ensure it was living. 5mLs of solution was removed from each well and dependent upon the condition, 10 mLs of fresh water was added corresponding to the letter on the well. Two fish from each group, A, B and C, were taken and put into a slide with water from their well and observed under a compound microscope. These fish were assessed on different levels: movement, speed, movement after being jostled or prodded, eye movement, heart beat, stage of development, curve of tail, swim bladder, fin size and pigmentation. They were then placed back into their wells using disposable pipettes. The remaining fish were again fed 15 microliters of brine shrimp with a P1000 pipette and two drops of paramecium.
Day 12: Same procedures from Day 8 were done.
Day 13: Same procedures from Day 11 were done.
Day 14: The one remaining live Zebra fish was put in tricaine and then formaldehyde. The rest of the wells were assessed and the fish still intact measured. The well and the water used for Groups B and C were then disposed of into the waste.
In the case of death: A disposable pipette was used to suction the fish out, throw it into the waste and suction out any leftover water. Then a paper towel was used to wipe the well clean.
Notes Taken: Day 0 Thursday 2/18/16 • Control (A1-A8) o Zebrafish H2- • Amoxicillin 858 mg (B1-B8) o 140 mg/ mL o crushed and dissolved in H2O (1 tab) • Metoprolol 25 mg (C1-C8) o 50 mg/mL o crushed and dissolved into H2O ♣ not fully soluble, still some particles present • Placed 2 mL zebrafish H2O in first 2 rows of wells • Placed 2 mL of dissolved amoxicillin in next 2 rows of wells • Placed 2 mL of dissolved metoprolol in last 2 rows of wells • Placed 1 embryo in each well
Day 1 Friday 2/19/16 • Checked on zebrafish @ 12:30pm o looked @ H2O levels, all look about 2 mL o all fish still living (based on observations made with the naked eye, not under scope) o all fish still in embryos; none hatched o some movement visible o Around 4:00pm looked at zebrafish under dissecting microscope ♣ took pictures (1 from A, B, C) ♣ all in embryos ♣ no movement seen
Day 4 Monday 2/22/16
- 48 hour stage - completely hatched - motile - A8 curved tail
- Control A7 & A8 deformed, have large mass behind eyes, non motile, tails bent abnormally - B1-B8 - normal development - very motile - done completely hatched - motile - B1, 2, 6 have specs at bottom of well
- C1-8 - mostly hatched - some sac left behind - little before 48 hour mark - C2,3,8 have specs in the bottom of well - C6 deformed a bit - All have very little motility
Day 5 Tuesday 2/23/16
• Control A1-A8 all in 48 hr stage, completely hatched motile • Control A7 & A8 deformed, have large mass behind eyes, non motile, tails bent abnormally • B1, B2, B5, B6 show normal development, very motile, 48 hr mark, particles at the bottom of the well suggest death & disintegration of another fish • B3, B4, B7. B8 normal development, very motile, 48 hr mark • C2, C3, C8 normal body development, little motility, appear "stuck to slide of well by face. disintegration particles at the bottom of the well are present • C1, C4, C5, C7 normal body development, no response when well was flicked, no movement seen • C6 deformity, nonmotile • egg shell removed from wells • all fish fed 15 mL of brine shrimp • more H2O added
Day 6 Wednesday 2/24/26 • 5:30pm • A8 dead, shell of body still visible, no disintegration • A1-A8 normal development, ~40 units @ 1.2x on dissecting scope, very motile • A7 nonmotile, deformed • B1-B8 ~40 units @ 1.2x, motile appear normal • C1-C5, C7, C8 all ~40 unites at 1.2X slowed motility delayed response to poking with transfer pipette • C6 deformed ~31 units @ 1.2x
Day 7 Thursday 2/25/26 • A4 o heartbeat steady, a lot of eye movement o Tail is about a 155 degree line o Bigger right fin o Yolk sac gone o Bump on front of fish and left near absence of yolk o Relatively still, moves when move water o Twitchy o Swim bladder enlarged • A2 o Relatively straight o Jumpy o Yolk sac gone o Swim bladder normal o Fins normal o Heart rate steady and normal o Fasts movements • B1 o Very straight o Slowed heart rate o Slowed eye movement o Normal development o Small fins o No yolk sac o Swim bladder normal o Little to no movement o Shaken, doesn't move o Relatively non motile • B3 o Perked up when moved o Jerks very quickly when taken o Straight o Slowed heart rate and eye movement when still o Normal swim bladder o Normal development o Small-normal fins o No yolk sac • C2 o Bone straight o Very still o Slowed heartrate o Darker tint o Small swim bladder • C8 o Fins a bit smaller than normal o Straight o More than 10 ocular spaces o Sac gone o Pretty still o Motile, but slow o Slow heart rate o Slow eye movement o Darker pigment
Day 11 Monday 2/29/16 • Notes from day: o Seems hotter in lab o look much worse from last week after not being fed o 4:41 pm o all extra info is because it is important day • A1 o Slightly curved o Motile, swims up and down tube when moved from well to slide o Moves when prompted o Slightly deformed • A2 – dead, disintegration beginning • A3 o Straight o Kind of small o Doesn't move much o Alive but doesn't move much ♣ EXTRA INFO • Swim bladder visible, normal • Slight curve in tail, favors side • Twitchy, yet still, very active when prompted • Besides that, mostly still, not a ton of movement • Heart beat normal • Eye movement normal • Head normal • Fins even, normal • Coloration normal • A4 o Really bent o More translucent than first two o Motile when jostled o Despite deformed tail moves relatively quickly ♣ EXTRA INFO • Right fin bigger • Twisted tail • Normal heart and eye movement • Mostly still, moves occasionally • Some movement too, seems jaw is normal, although favors right side • Moves mostly with fin instead of tail • Head normal • Jumpy, but not constant movement • A5 – looks dead, but no disintegration yet • B1 o Alive o Motile o Sluggish movement o Seemingly small ♣ EXTRA INFO • Normal heart rate • Straight • Normal fins • Very fast/ motile in sudden bursts • Eye movement fast • Smaller, more circular swim bladder • Normal pigmentation • B2 o Alive o Super active o Especially when tapped o Bigger than B1 • B3 o Straight o Still until tapped o Normal size o Pigmentation standard ♣ EXTRA INFO • Twitchy movement • Large, round swim bladder • Normal fins, fast movement of fins • Normal head, heart rate steady • Eye (really developed and motile) • Developed faster • B5 o Very active o Pigmentation clear o Straight • B6 o Non motile o Motile when jostled a lot o Kind of float around instead of swimming o Normal size • B7 o Alive o Normal size o Motile o More active when prompted o Normal pigmentation • B8 o Super motile o Clear pigmentation o No bend • C1 o Slight curve in tail o Very still o Barely active when prompted ♣ EXTRA INFO • Curved tail • Rapid fin movement • Pretty still • super developed and extreme movement of eyes • a lot of mouth movement as well • slightly darker pigmentation • swim bladder hard to see, under developed • movement when jostled • C4 o Clear o Deformed o Non motile o Non active when prompted • C7 o Slight curvature o Non active o 160 degree tail o barely motile when prompted • C8 o Tiny amount of curvature o Clear-ish pigmentation o Floats on side, might die soon o Move once when prompted ♣ MORE INFO • Super still • Virtually no fin or eye movement • Slightly curved • Really visible swim bladder, though still under developed • Really still • Slightly darker pigmentation
Day 13 Wednesday 3/2/16 • Measurements of fixed fish from day 7: o A6: 3,500 µm o B4: 3,125 µm o C5: 3,000 µm • (Kaila's specific observations, Alyssa please add the observations from extra) • A3 o Looks dead o Seems no heartbeat o No movement o Discoloration o Darker pigmentation o Warped eyes o Straight tail o Normal fins o Paramecium starting to develop around fish, eating at him • A1 o Strange o Really deformed o Possibly without fins, or really small o Discoloration o Less color o Small swim bladder o Does not respond to movement o Less me move without reaction o No curvature • A4 o Alive o Mostly non motile o Moves when prompted o Uses front fin to swim o Heart beat no visible o Normal eye and heart size o Not much eye movement o Normal coloration o Very bent tail o Swim bladder normal o Skin, or something coming off tail, maybe part of him is detached • B1 o Straight o Normal swim bladder o Normal fins o Not very motile o No discoloration o Not a lot of eye movement o Twitchy o Barely swims when poked o Sometimes swims of own accord • B3 o Straight o Darker o Normal swim bladder o Right fin smaller o Minimal eye movement o Thin o Eyes look lighter than black o Not super motile o Doesn't swim when poked • C1 o Normal swim bladder o Starys on side (sort of floats) o Black eyes, no movement in them o Non motile even when prompted o No curve o Darker pigmentation o Smaller fins • C4 – disintegrating, curved, dead • C7 o Dead o Surrounded by paramecium o Normal size o No curvature
Day 14 Thursday 3/3/16 • A1,3,4 were all found dead o Officially all controls are dead o A3 & 4 were getting eaten by the paramecium ♣ Alyssa has a picture of A3 o No control fish was fixed on Day 14 because all were dead • B1, 3, 5, 6, 7, and 8 were all found dead • B2 got fixed o Measurement: 3,575 µm • C1 and 7 were found dead o No C fish was fixed on Day 14 because all were dead
March 16, 2016
Title: Vertebrates and Invertebrates, how do they affect Transect 1? Purpose: To identify the vertebrates and invertebrates in Transect 1 and analyze their part in the food web.
Materials and Methods:
We visited the transect and collected leaf litter and samples from five different plants followed by setting up the Berlese funnel in which 25 mL of the 50:50 ethanol/water solution was placed into a conical tube. A piece of screening material was placed into the bottom of the funnel and it was placed on a ring stand and the base was covered with parafilm. A lamp was placed above the funnel and the entire structure was covered with foil. After a week it was taken apart and the liquid was decanted into a petri dish. The remaining liquid was then poured into a different petri dish. The petri dishes were placed under dissecting microscopes and observed for the presence of invertebrates. Any organisms seen were identified and characterized using a dichotomous key and differences due to their location in the funnel were noted (Bentley, 2016).
Data and Observations: Vertebrates Birds, Squirrels, Raccoon, Skunks, Deer
Invertebrates Figure 1. Aphids and Louse under microscope Figure 2. Example picture of an Aphid Figure 3. Beetle under microscope Figure 4. Example picture of beetle. Figure 5. Sucking louse under microscope Figure 6. Example picture of sucking louse. Figure 7. The Foodweb of Transect 1
Conclusions and Future Directions: The Foodweb (Figure 7) shows the huge amount of interrelations among the micro ecosystem of Transect 1. The way the bacteria eats the things that die like foliage and animals and the bugs protozoa eat bacteria and insects eat protozoa. The projected vertebrates in the transect would also play into this, as the squirrels would eat nuts coming off of trees and the birds will eat insects. It becomes a type of cycle. I think the search for the invertebrates would have been better in a different season. The temperature and conditions not only changes the homeostasis of the ecosystem, but also greatly affects eating habits and the types of organisms we see because not all of the weeds and angiosperms are in full bloom.
February 17, 2016
Header: Intricacies of plant life in Transect 1
Purpose: To observe and determine the greater intricacies of Transect 1 plant life: vascularization, reproduction structures and etc.
Materials and Methods: We went out to our transect and collect various samples of five different types of plants. We tried to either collect roots or collect seeds to determine as much about the plant as we could. Then we measured the leaves and length of the plants, followed by performing a cross section and examining the plants under dissecting microscopes.
Data and Observations: Plant 1: Plant 1 has long thin leaves, that feel smooth almost like plastic to the touch. We managed to get the roots for this plant and it can be seen that the leaves spread out much like the roots. Plant 2: Attached to a tree with a taproot system. Plant 3: Also a taproot system as it goes straight down in spreads from central root. Plant 4: Plant 5: None of the plants were currently flowering so we didn't get any seeds. But I made some educated guesses to whether they were monocot or dicot if they were a plants that produced seeds.
Figure 3.1 Plant 1 Figure 3.2 Plant 2 Figure 3.3 Plant 2 Measured Figure 3.4 Plant 3 Figure 3.5 Plant 4 Figure 3.6 Plant 5 Figure 3.7 Characterization of Plant
Discussion and Future: Our transect is pretty worn down and doesn't have much variation in plant life. There isn't really a water source and it is pretty standard in lack of differentiation in plant life for a college campus. This makes it particularly hard to find organisms and analyze what is actually going on. For future it might be better to do this in a different season, specifically not winter when it is hard to tell if common weed plants are indeed flowering or not. Furthermore maybe switch to a transect where there is more flowering plants or a water source so there is more variation in vascular systems or even reproductive methods. We also found a fungi. Fungi sporangia, it is medium, dark, circular waves that held spores. It was characterized as Tremella folicea, gelatinous and quite wet in feel.
Works Cited: Meg Bentley, Sarah Knight, Nancy Zeller and Kathryn Walters-Conte. "Biology 210 Laboratory Manual." iBooks.
February 10, 2016
Header: Serial Dilutions, Bacteria Analysis and Gram Stains
Purpose: To identify bacteria in our transects hay infusion and figure out what they are, how they move and how they're made up.
Materials and Methods: Wet Mount: This process was repeated multiple time for four different bacterial colonies. When examining the Agar plates, with and without the presence of tetracycline, we decided which colonies looked promising or interesting and took about half of the colony on a sterilized loop. We then added it to a microscope slide with water, about a drop, and mixed the two. We looked at it under a compound microscope and tried to identify a bacteria if one was present.
Data And Observations: We noticed that the transect materials we have been getting of not produce a large quantity of bacteria when we go to put it under the microscope. We think there is something specific to our transect. Either way, we only found 2 organisms and the gram stains were not effective. The pictures below in figure 2.3 and 2.4. Agar_Plates.JPG Figure 2.1 Agar Plates, Dilutions and Tetracycline and Not Dilution.jpg Figure 2.2 Dilution and Conversion Factors GramStainPositive.JPG GramStainNegative.JPG
-Kaila Bryant
Biotic Components Clovers Acorns Sticks (Branches) Flower (Kerria Japonica "Pleniflora") Leaves (Oak)
Abiotic Components Rock Soil Wind (11 mph) Temp (28 Degrees F) Light
Transect Description The ground is packed hard with moist soil. There is s light splattering of leaves everywhere. There are trees of varying size, the large ones are oak. The light post is the the left of the middle and surrounded by trees, plants and leaves.


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San Francisco, CA, May 25, 1988 --- Democratic presidential candidates Michael Dukakis (L) and Jesse Jackson try to make a point at the same time during their televised debate 5/25/1988. --- (UPI Photo)
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Mozilla Join on HFI End Semester Blast
Last October 29, 2011,I've been invited to Holy Family International Colleges (HFI) to do a short talk about Mozilla which is located at the province of San Mateo, Bulacan an two hours travel from Manila. The event was a success and looking forward for a next project with the HFI since it is just a newly open school in their province.
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Just how to Write An Investigation Release
Santa Clara University Thursday, Jan. 23, 2014 Friday, Bing removed two Chrome visitor extensions (feel apps added to your online browser) from its shop after they were discovered to be installing unwanted software and redirecting customers to affiliate links. Both extensions, Tweet this Page and Send to Feedly, offered cost-free, created by personal developers and started as respectable providers. Into a organization who buy-essays.online review then took advantage of present subscribers to share ads, the expansion was offered by the initial programmer in both instances. Amit Agarwal, deliver to Feedlys president, offered his extension used 000, by 30 to an anonymous occasion. It was a 4-physique present for something which had consumed one hour to create and that I agreed to the offer, says Amit. A apologizing has been since released by him to active users, and mentioned that acquiring the offer was a negative conclusion. Nonbusiness agencies make a great deal of these solutions and are offered cost-free, although several businesses distribute applications and extensions.
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Fox stuurt 'The Day the Earth Stood Still' de ruimte in
Twentieth Century Fox heeft besloten om The Day the Earth Stood Still de ruimte in te zenden. Een primeur gezien het de eerste keer is dat een film onze alien gebroeders tegemoet zal gaan.
De uitzending begint komende vrijdag, gelijk met de release van de film in Amerika. Levende wezens in het Alpha Centauri zonnestelsel zullen de film dan over een viertal jaar ontvangen in 2012. Er van uitgaande dat ze de juiste technologie hebben om de signalen te ontvangen en ontcijferen.
De mensen van Twentieth Century Fox schrijven nog lachend in hun persbericht dat het een extra vier jaar zal duren eer we de eerste besprekingen van onze galactische buren zullen ontvangen.
Ik begrijp het nut er helemaal niet van, maar ik vind het wel een enorm coole gedacht, om één of andere reden. Of The Day the Earth Stood Still de juist film is om de ruimte in te sturen laat ik even in het midden.
De reacties op Comingsoon, vanwaar ik bericht heb, zijn wel grappig:
They want to make first contact with a movie about aliens and humans killing each other? I hope to god this movie has a peaceful resolution. At least they aren't sending out "Independence Day."
En deze:
Ah yes, nothing says "Earth has Intelligent Life" better than two hours of Keanu Reeves dead panning to the camera.
Zouden de film met ondetitels verzonden worden? Laat het aan een Amerikaan om te denken dat de rest van het universum ook Engels spreekt. Misschien interpreteren ze de muziek als de hoofdtaal, of denken ze dat zowel de gesproken taal als de muziek een geheel vormen?
Wat als het dove wezens zijn, wezens die communiceren met telepatisch krachten. Of blinde wezens, dan hebben ze er ook niet veel aan.
Hoe het afloopt zullen we ten vroegste over vier jaar merken, of langer. Hopelijk beledigen we niemand met de boodschap van de film. Was het niet, volgens de kalender van de Maya's, in 2012 dat de wereld zou vergaan?
The Day the Earth Stood Still is sinds vandaag in onze zalen te zien. Geniet er van.
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|807 sq. ft.
|Property type
|Minimum Stay
|3 - 7 nights
welcoming and sunny house of 75 m2.
southwest exposure.
Description of property: House located in the yard of a private residence not close.
Small strip of land unfenced private two meters wide in front of the house, surrounded by a privet hedge.
No private garden.
No direct view of the sea.
Park 100m.
Private parking space adjacent to the house.
The sea is accessible by the court to 50 meters.
Pets allowed under certain conditions to do with the owner.
Interiors description:
Entrance :
1 kitchen and 1 utility room.
, Living room of 28 m2 with a large adjustable table for 6 to 8 people with 6 chairs, high chairs, a three seater sofa (not convertible) with a small coffee table and 1 big chair.
Upstairs: - 1 bedroom street side of 13 m2 with a bed for 2 persons 140x190 and dresser.
- Bedroom 2 of 16 m2 courtyard with a bed for 2 persons 140x190, a BZ style sofa bed for 2 persons 140x190 and wardrobe.
- 1 Cot umbrella style.
A bathroom with tub shower and sink basin.
A separate WC.
Sports and leisures :
- cinema
- casino
- Beach club for children
- QI quong on the beach in July and August
- Hiking trails
- Boules
- riding stable
- Shooting center
- Friendly boaters fishermen
- Sailing clubs
- Scrabble club
- tennis Club
- Thalassotherapy center at Luc sur Mer
- Aqua center pearl in Dover Deliverance
- Museum of Arromanches
- Shore to landing
- Memorial in Caen
- Pegasus Museum etc .....
A Saint Aubin sur Mer, ranked station tourism,
charming little house in a private residence in the southwest of exposed seaside.
Although located on the seafront with direct access to the beach.
1.8 km long seawall promenade connecting a side Bernières sur Mer and the other Langrune sur Mer.
You will come to discover its beautiful changing colors of the sky and the sea and the sunsets. You will enjoy the surrounding markets where you will find all the fish and vegetables days
Saint-Aubin-sur-Mer, ranked tourist resort nicknamed 'The queen of iodine' welcomes you to enjoy the fresh air, calm and friendly atmosphere prevailing there. Many attractions and landmarks landing await your visit.
Ideal location: 10 minutes from Caen, 3 hours from Paris, 1 hour from Deauville, thalassotherapy center 5 minutes, nearby casino, cinema 50 meters, beach club for children and aquatic center at Dover The Deliverance.
http: // www. aquanacre. en / index. php
Ideal for family holidays.
Chantal, the owner of the house, communicates very friendly via email and phone during the booking process. When we arrived she welcomed us warmly and showed us in detail her lovely house. The house was perfect for us as a group of three adults. It's very nice decorated, very clean and well equipped! All we needed was available. And off course the area is great, less then a minute walk to the beach, the village and restaurants nearby. We stayed during the D-Day memorials and this house was a perfect place for us to start our daily tours for D-Day sightseeing as it's located directly at Juno beach, the Canadian landing beach. I can't make up anything to improve, we loved the house and we would love to come back!
Kind regards,
We were welcomed by Virginie ,made to feel at home immediately!!! The cottage was exactly as described ,very clean,well decorated and supplied with anything we could ever need!!! We were four adults staying at the cottage,all the information and directions for St-Aubin were right on!! Again I thank Chantal for welcoming us into her cottage and for making it easy for us to discover "La Normandie" and visit Juno Beach.
Merci mille fois
Chantal was very nice and flexible with us on the dates of the vacation, which was hard to find as most people rent from Sunday to Sunday only. She also allowed us flexibility on the hour of arrival as we had a long drive to arrive there and happily answered all our questions. The house was as described, beautifully decorated and very clean. We stayed with 3 children who slept together in the same big room. We all liked the house and felt very comfortable. The kitchen is very well equipped. Although not in the front line, from the property to the beach it is less than a one minute walk. A garden with a small playground near by, several restaurants on the beach and grocery, bakery, butcher and vegetable shop in walking distance. I would happily go back there and highly recommend it.
Great welcome by host Chantal. House was terrific: good space, clean, extremely well supplied and maintained, off street parking, nicely decorated and furnished. Location was literally steps away from the beach promenade and beach. Chantal seemed to anticipate most anything a family would need to enjoy a beach front vacation. There were several restaurants within walking distance with reasonable prices and selections. We visited in the off-season (April) so some seasonal businesses had not yet opened. We used the location as a central point to for exploring the Normandy coast and inland areas. There is much to see including the World War II sites and iconic French attractions like Mount St. Michel. In total, the house was terrific and was key in making our Normandy visit a great experience. Thank you, Chantal for introducing us to St. Aubin-Sur-Mer. We would recommend this property to family and friends.
This was a lovely little cottage perfect for our family of three. It was nicely decorated with a very comfortable couch and beds. It also has everything you could possible need. Chantal keeps it stocked with games, sand toys, beach shoes, laundry detergent, basic food items (seasonings, pasta, oil etc.,) and every kitchen gadget and item you would need. We stayed for four days and were very comfortable. Lots of great light, super close to the beach, close to restaurants and shops. We used it as a base for exploring Normandy and spent one day just relaxing and playing with our toddler on the beach. Perfect! We would love to return after our new little one arrives. Merci, Chantal!
Can't say enough about this wonderful cottage. We spent 5 wonderful days here with another couple. Chantal made everything so easy- she was charming and helpful, including excellent recommendations for restaurants and nearby grocery stores. The cottage was the perfect spot for our daily travels to the beaches of Normandie and to Caen. Even rode horses on the beach. Thinking of booking this house? Don't hesitate - it has everything you need for a comfortable, cozy stay! Merci Beaucoup, Chantal!
|No additional mandatory fees
|Stay tax
|$0.71 per night, per person. This amount will be due to the owner upon arrival. Does not apply to children under 18 years.
Conditions de paiement:
- Caution de 400 € à déposer le jour de l'arrivée,elle n'est pas encaissée et rendue le jour du départ, après état des lieux de sortie.
Charges en plus :
- la taxe de séjour qui est de 0.60 € /jour/personne (sauf enfants de moins de 18 ans)
- chauffage en plus sur relève des index du 1 novembre au 31 mars .


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Speaker of the House of Representatives, Hon Dimeji Bankole could not hold back tears on being confronted with the menace of juvenile delinquency in the nation's at the Borstal Remand Home, Adigbe, Abeokuta.
The event occurred weekend during his campaign for re-election to represent Abeokuta South Federal Constituency when he went to the Remand Home to commission an ICT centre he attracted to the reformative institution as part of his constituency projects.
Breaking down emotionally and finding it difficult to address the young inmates, Bankole could only manage to seek for a future audience to interact with the delinquents, noting that he would want to be fully briefed on the reform process.n of ills.
Bankole said with only three of its kind in the country, he did not want to believe there were no juvenile delinquents in other parts of the country, stressing that "our society will be better off if we can address the ills of the society at this early stage before attitudes are fully formed."


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Current Weekly Ad at Target in Chicago
Founded in 1902, Target is the second largest retailer of discounted goods in the U.S. They are now expanding grocery selections in many of their stores. Their weekly ad with specials and deals is available online for your Chicago store. Read more information on Target.
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Apr 27, 2017 -
Apr 29, 2017
Starts 12:00 and Ends 18:00
(UTC-05:00) Eastern Time (US & Canada)
North America
Captiva, Fla.
View Event Registration Details
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text:
WACO, Texas � It took just two batters Saturday to realize Arkansas was dealing with a different offense than the ones it faced during last week's Houston Regional.
WACO, Texas � It took just two batters Saturday to realize Arkansas was dealing with a different offense than the ones it faced during last week�s Houston Regional.
Baylor�s pesky designated hitter, Nathan Orf, was hit by a pitch for the 35th time this season to lead off the Waco Super Regional.
Then, center fielder Logan Vick deposited a pitch over the wall in left centerfield.
The Bears beat up Arkansas ace D.J. Baxendale in the early innings and cruised to an 8-1 win during the first game of the Waco Super Regional in Baylor Ballpark on Saturday. Baylor, the NCAA Tournament�s No. 4 national seed, clubbed three home runs and four doubles, shelling a staff which allowed one earned run in three games last week.
It moves the Bears (49-15) one win away from reaching the College World Series for the first time since 2005. Arkansas (42-20), meanwhile, faces a do-or-die game when the teams meet at 3 p.m. today.
�Baylor just kind of took it to us (Saturday),� said Arkansas coach Dave Van Horn said, summing up his team�s disappointment.
Arkansas knew it was facing a talented offense before the super regional began. Baylor entered the game hitting .312 as a team and .327 during home games this season.
The Bears lived up to those expectations, doing most of its damage against Baxendale.
�I made a lot of mistakes that Baylor took big-time advantage of,� Baxendale said. �But they�re also a great hitting team. They hit a lot of good, solid pitches. They�re tough.�
It left the right-hander � who was a 10th-round pick in the Major League Baseball Draft earlier last week � suffering through his worst outing since early in the season.
The trouble began with the 2-0 hole thanks to Vick�s first-inning home run. But catcher Josh Lundy added a solo shot - his 16th home run - in the third inning to add to the lead. Vick hit his second home run of the game in the fourth to help the Bears build a 5-0 lead.
�Everyone was hitting good during the week,� said first baseman Max Muncy, who went 3-for-4 Saturday. �We came into this game and prepared for this guy and he was throwing it right where we thought he was going to and we put some good swings on the ball.�
It was unusual for Baxendale, who had allowed just one earned run in his previous 20 1/3 innings pitched in NCAA Tournament play.
The three home runs hit off Baxendale (7-5) were the most he had allowed during one outing in career. Baylor also tied the season high for home runs allowed by Arkansas, equaling LSU�s output in a win on March 30.
�I thought from top to bottom offensively we were hooked up pretty good,� Baylor coach Steve Smith said. �Which, coming into a game like this, is what you�d expect.
�If you can�t be hooked up for this, when are you ever going to be hooked up?�
Van Horn said part of the problem was the fact Baxendale didn�t have the usual command of his fastball Saturday. But whatever the issue, Baxendale�s struggles were problematic for an Arkansas offense not known for its ability to put together big innings.
Baylor pitcher Trent Blank (11-1) made sure it remained true Saturday, limiting the Razorbacks to one run on seven hits in eight innings. He got some great defense from shortstop Jake Miller and was aided by a base-running error by Arkansas first baseman Dominic Ficociello, who was thrown out at third base on a grounder in the second inning.
Outfielder Matt Vinson followed with a single up the middle the next at-bat.
�We�re right there,� Van Horn said of the 2-0 deficit at the time. �What can I say?�
But Blank also worked his way out of other jams to keep Arkansas scoreless.
The Razorbacks didn�t break through until the seventh inning, when Ficociello�s third hit of the game was an RBI double. The run only trimmed the deficit to 6-1, though.
Baylor tacked on runs against Arkansas reliever Ty Wright in the seventh and eighth innings to extend its lead. Crayton Bare came out of the bullpen for the ninth inning and finished the game, wrapping up the win.
�We just didn�t have it and it showed on the scoreboard,� Baxendale said.
The good news? Arkansas is no stranger to falling behind in a three-game series.
The Razorbacks lost the opening game of a series seven times in 10 weeks during Southeastern Conference play. Arkansas rebounded to win the second game five times and won the series three times.
�If I know the team like I think I do, then don�t expect us to go out (today) without a fight,� Baxendale said. �Expect us to claw nine innings playing as hard as we can.�
Arkansas won�t have an easy road, though, facing Baylor pitcher Josh Turley. The left-hander is regarded as the Bears� ace and will start opposite Arkansas� Ryne Stanek.
But Ficociello believes the Razorbacks will shake off Saturday�s frustrations and force a deciding game on Monday.
�A lot of times you have to lose to motivate yourself a little more,� Ficociello said. �We didn�t come out ready enough (Saturday). So (today) we have to show everybody that we deserve to be here. Because we do.�


text:
All your favorite stars naked
Pamela Anderson reached the top of her celebrity in the 90's. She was one of the attractive baywatchers of the Baywatch television series and was the protagonist of one of the first sex tapes appeared on the net. This success took her to the cinema, where she played Barb (…) Read More
© 1999-2017 CinemaCult.com -


text:
Edward Harris House Bed & Breakfast Inn
This 1896 boutique style Inn is a retreat of urban serenity. Recently voted one of I Love Inns and American Historic Inns '2009 Top 10 Most Romantic Inns of North America' The Edward Harris House B&B Inn provides our guests with the quiet, private relaxation of a neighborhood setting and the convenience of a historic central city location.
Edward Harris House Bed and Breakfast
35 Argyle Street
Rochester, New York, NY 14607
35 Argyle Street
Rochester, New York, NY 14607
New Cottage Space
read more


text:
Once upon a time I created a small (tiny) lotions business that I named The Naked Girl. The double meanings here were that the lotions were all-natural, aromatherapy infused moisturizers and hand lotions and that my autistic daughter liked to be naked—and who inspired the name. It took me years—literally—to make her wear clothes and to keep them on. We could be anywhere, mostly home, and she would just decided to disrobe. And she was quick too, someone once likened her to Houdini. Kind of cute when she was 2-3-4 and 5, but at 13 and now a fully developed woman—NO WAY! Not cute, only disturbing!
I think by now you probably know where I'm taking this horror story. I forewarn you, the following does contain nudity:
Yesterday we were at a casual restaurant located on the beach in my hometown. Yes, very casual, casual enough to grab dinner with nothing more than your bathing suit and a pair of flip-flops. But shirt is required and absolutely no "exposing of the breasts" (not actually a sign stating this fact, but goes without saying, eh?) Meghan was sitting and waiting for her dinner to arrive and gave her father the sign for the bathroom, but I had stepped away for a moment and he told her to wait. I guess she didn't want to wait, Daddy, because she pulled down her one-piece bathing suit to full breast exposure! And not once but twice—to make matters worse. Her father had to use all his might to pull it all back up. "OMG!" Well that was the kind of hullabaloo that could be heard throughout the, by then, big crowd of people simply waiting for their fish and chips, and beer and wine—and no nude shows, please! Except for the Chinese family sitting in the corner, they thought it was the funniest thing they'd ever seen, especially little old grandma—at least we made somebody's day?
Needless to say, while the little old lady was "forcing out" a lot of hot air, I was "sucking in" a lot of cold wine!! And let it be said that we were probably the conversation starter at a few too many breakfast tables this morning.
Oh, what an addition to the family memoirs!!
Can anyone relate?
The best personality type game I've ever played
9 hours ago


text:
Abbott Key Account Manager II - Hospital Channel - Abbott Diabetes Care in Raleigh, North Carolina
At Abbott, we're committed to helping you live your best possible life through the power of health. For more than 125 years, we've brought new products and technologies to the world -- in nutrition, diagnostics, medical devices and branded generic pharmaceuticals -- that create more possibilities for more people at all stages of life. Today, 94,000 of us are working to help people live not just longer, but better, in the more than 150 countries we serve. Please note that candidates need to be eligible to work in the U.S. without Abbott sponsorship.
Primary Function
The Key Account Manager is a generic position held in various sales channel and is accountable for achieving sales objectives through profiling, targeting, needs analysis and closing of sales on targeted strategic account customers and ensures the sales are profitable and within the market goals of ADC. The position is responsible for the development, management and growth of the healthcare professional recommendation to achieve sales and market share growth maximization within a strategic account setting. The major objective for this position is to grow Abbott Diabetes Care business and brand loyalty within strategic accounts including key HCP accounts, government accounts containing large diabetes clinics, hospital teaching institutions, and other major medical centers noted throughout the area for their expertise in training medical professionals and the diagnosis/ treatment of diabetes. He/she will call on these institutional and key HCP customers to raise their level of awareness and increase the demand of the ADC product line. The KAM will help to plan, direct, and coordinate overall sales activities and strategies within the given geographical area of these accounts, while increasing sales, profits and market share on a long term basis. He/she is required to maintain an active and visible role with Hospital, HCP, retail, managed care, and strategic account representatives in order to facilitate pull through programs to increase awareness and demand for the ADC product line. This position will bear the responsibility of correlating any professional contact needed within the above mentioned accounts with the operational, quality or customer service teams of ADC.
Main Responsibilities
Achieves/exceeds sales objectives within assigned geographic territory by maintaining current diabetes base while increasing market share.
Develops and maintains superior relationships with key diabetes decision makers and influenciar. Effective in implementing customer loyalty initiatives.
Exhibits proficiency in demonstrating all diabetes products, as required.
Exhibits proficiency in demonstrating and training on all ancillary data management software in Abbott Diabetes Care product family.
Maintains a high profile with decision makers of the assigned strategic accounts.
As required maintains an active role with the local regional Hospital team, HCP team and Managers.
As required maintains an active role with the Managed care Team.
As required facilitates pull through of programs with the retail teams.
Arranges any training required within the strategic accounts.
Maintains a high profile with the professional diabetes organizations in the assigned geographical area.
Contacts and manages retail promotions to pharmacies located on the premises or connected with the assigned strategic accounts.
Completes all administrative duties in a timely fashion
Maintains a high degree of professionalism with customers and peers.
Responsible for compliance with applicable Corporate and Divisional Policies and performing other duties as assigned by management Note: This job description describes the principal and main elements of the job. It is a guide to the nature and main duties of the job as they currently exist, but is not intended as a wholly comprehensive or permanent representation
Accountability / Scope / Budget
This position interfaces frequently with Field Sales, Marketing and the company's Customer Base. It requires a comprehensive knowledge of the sales and distribution aspects of the glucose monitoring business. It also requires competent knowledge of the interior structure of the Alameda location. Travel schedule may exceed 50. Customer focus is frequent. Compensation and recognition are tied directly to personal performance. Involves considerable freedom to structure and manage work independently. Evaluating information critically and making decisions based on the data are important. Social confidence and the ability to speak in a clear and articulate manner are important. Ability to read behavioral cues and establish/maintain close business relations with high level health care professionals is critical. Ability to persuade and influence without direct control is also important.
Minimum Education & Experience
Bachelors degree or equivalent experience.
One calendar year experience with ADC or appropriate experience as required.
Excellent strategic planning and project management REQUIRED
Strong written and oral communication skills
Strong interpersonal skills.
A high degree of social confidence and presentation skills.
A high degree of computer literacy.
Must be willing to travel 50 during the work week.
Must be willing to reside within the geographical area of the assigned area.
4-6 years of sales experience.
This position may requires the ability to travel, up to 60%.
Sales Force
ADC Diabetes Care
United States of America : Remote
Yes, 50 % of the Time
Driving a personal auto or company car or truck, or a powered piece of material handling equipment
Abbott is an Equal Opportunity Employer of Minorities/Women/Individuals with Disabilities/Protected Veterans.
EEO is the Law link - English: http://webstorage.abbott.com/common/External/EEO_English.pdf
EEO is the Law link - Espanol: http://webstorage.abbott.com/common/External/EEO_Spanish.pdf
Abbott is about the power of health. For more than 125 years, Abbott has been helping people reach their potential — because better health allows people and communities to achieve more. With a diverse, global network serving customers in more than 150 countries, we create new solutions — across the spectrum of health, around the world, for all stages of life. Whether it's next-generation diagnostics, life-changing devices, science-based nutrition, or novel reformulations, we are advancing some of the most innovative and revolutionary technologies in healthcare, helping people live their best lives through better health.
The people of Abbott come to work each day with relentless energy, enthusiasm and a promise to enhance the health and well-being of millions of people. They push the boundaries to help manage and treat some of life's greatest health challenges.
We invite you to explore opportunities at Abbott , to see if your talents and career aspirations may fit with our openings. An equal opportunity employer, Abbott welcomes and encourages diversity in our workforce.
Follow Us:
Facebook at https://www.facebook.com/AbbottCareers
LinkedIn at https://www.linkedin.com/company/abbott-
YouTube at https://www.youtube.com/c/abbott
Twitter at https://twitter.com/AbbottNews


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Best Ways to Get You An Investor Who Can Buy Your House In Cash Many people talk about success with flipping houses and how they can do the same thing. The money they don't have it right now is what...
Read more
Best Ways to Get You An Investor Who Can Buy Your House In Cash Many people talk about success with flipping houses and how they can do the same thing. The money they don't have it right now is what you always hear from different walks of life. Real experts in the real estate world didn't have any money when they started either. Same people would ask them if where they got the money. The only answer to this question are the investors. The question now that comes into your mind probably is how to find an investor to buy my property? Investors are anywhere and you can meet them. They are just people that currently are not satisfied with the return they're getting on their retirement account or their savings account. At the foreclosure auctions you will find them every week. At educational conferences and seminars you will always find them. You will meet them at most real estate investing meet ups. They are at entrepreneur and small business meet ups. They are on popular investing websites or on other online investing sites. Your friends and family might be one of them. In all of these places you can find investors. To be honest finding them is the easy part. You're trustworthy and that you'll be able to to grow their money can be a little bit more difficult to do to convince them. So here are the two most successful strategies that you can use when trying to win the hearts and the cash of your future investors.
The Best Advice on Houses I've found
You need to have the knowledge and the confidence that you are the right place for this investor's money. Waiting for yourself to finish reading twenty books on real estate, sales and motivation is not necessary. You don't need to knock on a bunch of doors and talk to sellers. You don't need to go to a plenty of real estate investing meet ups. You don't need to hear a bunch of podcasts. Without doing all of these you will have enough knowledge to be able to convince an investor you know what you are talking about. This is important because investors want to know their money is safe. If you know what you are doing obviously these investors will think that it is safe for them to trust you their money.
A 10-Point Plan for Houses (Without Being Overwhelmed)
You need to be able to add value to people. Needing to do it more often. All the different real estate strategies that you can talk for an hour is not a need to do even if it is your first time talking to the investor. These people are familiar with real estate. There is something else other than just these strategies is what you want to do for you to let them see your point. There could be more profitable than renting is what it means to them. You will be granted with their trust and money if you have the knowledge to pull this off. What they see in you the excitement is important. When people see that you are genuine and you would gladly give more time to help them and invest in them they will obviously be more inclined to invest in you. It is easy to find an investor but are you ready to face your investor? To help you is the only goal of this guide.


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I spent the last few weeks in and out of town. First to Far West Texas to spend some time with my dad, and then to upstate New York to pick up our daughter from her first year in college.
Now I'm putting photos and thoughts together to share about my time out west. In between enjoying the amazing, early-May, 70 degree weather that is, and tooling around the gardens to see what's coming up.
Spring has sprung in Maine - always a happy time!
Copyright © 2013 - Paulla Estes


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- Masafumi Hashimoto, Ryunosuke Izumi, Yuto Tamura, Kazuhiko Takahashi
- 2014 11th International Conference on Informatics…
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This paper presents laser-based tracking of moving objects conducted by a group of mobile robots located near one another. Each robot finds moving objects such as people, cars, and bicycles in its own laser-scanned images using a binarized occupancy-grid-based method. It then sends laser measurements related to the detected moving objects to a central… (More)
- Yuto Tamura, Hiroyuki Saeki, +5 authors Hiroko Yamada
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A p-i-n organic photovoltaic cell with tetrabenzoporphyrin (BP), a BP-C₆₀ dyad and PCBM for the p-, i- and n-layers, respectively, gave a better fill factor and power conversion efficiency than the corresponding p-i-n cell having a 1:1 blend film of BP and PCBM as the i-layer.
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Mother's Day is May 13th
Sweetly Spring Basket
Flower Arrangement
This medley of purple blooms is guaranteed to put a smile on your Mom's face! A surprise gift of flowers can provide a "pick me up" that lasts for days, so boost her spirits to the sky with this Sweetly Spring Basket from WHITE FLINT FLORIST, your local Gaithersburg, MD florist!
$67.00, $79.00, $91.00
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Effervescent Blooms
Giving Mom Beautiful Fresh Flowers Will Fill Her Day with Sunshine and Love. To Send a Mother's Day Arrangement in the Gaithersburg, MD area, Call WHITE FLINT FLORIST Direct or Order Flowers Online 24/7.
$108.00, $120.00, $132.00
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A mother's love knows no bounds. Show her just how much you care with a special Mother's Day flower delivery. WHITE FLINT FLORIST has the best selection of flowers and gifts for Mother's Day. Send a bouquet of her favorite flowers, or have your florist create something stylish to fit her personality! When it comes to flowers, the possibilities are endless. Come by the shop, give us a call, or click on any of the flower arrangements above to get started.


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P1.1 . 20 May 2009
Contents • People and Places • Rotterdam Periscope • SmartGeometry Conference 2009
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Architectural Products Update Concrete Log Homes EverLogs™ provide a long-lasting alternative to wood logs for construction of homes and commercial buildings. Made of reinforced, insulated concrete, the building system matches the character and texture of wood logs while providing resistance to fire, insects, and decay. In additional to having high thermal mass, each "log" contains two inches (five centimeters) of insulation. Assembly by EverLog Systems ensures an exacting fit, further reducing heating and cooling costs compared to those of wood log homes. Log packages include erection costs. Can be manufactured to specification. Available in a variety of textures and natural wood colors. everlogs.com Discuss this article in the Architecture Forum...
FSC-Certified Bamboo Plywood & Flooring Smith & Fong Co. introduces a line of Plyboo® bamboo architectural plywood and flooring products that are Forest Stewardship Council (FSC)-certified. The manufacturer has established a responsibly managed bamboo forest resource that qualifies the product line for the FSC Pure designation, since the products consist of 100-percent FSC material from an FSC-certified forest, and they have been sold and/or processed by an FSC chain-of-custody certified
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company. In addition, the manufacturer's bamboo sources use no irrigation, pesticides, or fertilizers. Can contribute to LEED credits: MR 6: Rapidly Renewable Materials, MR 7: Certified Wood, and EQ 4.4: Low Emitting Materials (no added formaldehyde). plyboo.com
Wood Finish from Whey PolyWhey™ wood finishes from Vermont Natural Coatings are formulated using whey protein, a natural by-product of the dairy industry, as the bonding agent. The finishes are very low in volatile organic compounds (VOCs) — at or below 180 grams per liter — helping protect indoor air quality. These scratch-resistant clear finishes cure twice as hard as the average water-based finish and do not yellow in UV light. Excellent flow and great coverage. Dry in two to three hours. Available for floors or furniture. Can contribute to LEED credits. vermontnaturalcoatings.com
Green Shading System The Mecho®/5 manual solar shading system with EcoVeil® shadecloth is Cradle to Cradle silver-certified. The non-PVC screen cloth can be reclaimed and recycled, and is durable, washable, anti-microbial, and flame-retardant. It has a core of thermoplastic olefin (TPO) fiber filaments, with a TPO coating. Available in a range of colors. Solar-shading systems reduce solar heat gain and glare while transmitting daylight, thus facilitating increased building energy efficiency and occupant comfort. Mecho®/5 is one of the chain-driven roller screen systems available from MechoShade Systems. mechoshadesystems.com
Integrated HVAC System The Matrix Total Home System from NY Thermal Inc. is a single appliance that combines heating, water heating, and heat-recovery ventilation. The system includes a condensing water heater, condensing furnace, condensing boiler, and heat-recovery ventilator. It is also prewired for air conditioning. Compared to conventional natural gas furnaces and water heaters, the device is 60 percent more efficient in heat recovery, 30 percent more efficient in water heating, and is more efficient in space heating. It also uses less electricity than the total consumption of the component appliances in a conventional configuration. Exceeds the Canadian Standards Association (CSA) P.10 performance standard for integrated mechanical systems. ntimatrix.com
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Published on May 20, 2009
EverLogs™ provide a long-lasting alternative to wood logs for construction of homes and commercial buildings. Made of reinforced, insulated...