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Perspectives on Intellectual Capital
Army Techniques Publication ATP 3-91 Division Operations October 2014
Develops, coordinates and implements agency-wide work life policies and programs. Establishes and manages interagency agreements and contracts for the agency health services program, fitness services program, employee assistance program, ergonomics and AED program online. Capital is any product that is has utility for production. Human resources are the labor, skills, and knowledge of persons. Exchange is the trading of goods for money or for other goods. A market is any mechanism for buyers and sellers to exchange goods Knowledge Management: Social, Cultural and Theoretical Perspectives (Chandos Knowledge Management) 6on6.playground.wedeking.org. The three major categories of benefits managed by HRM managers are: employee services, such as purchasing plans, recreational activities, and legal services; vacations, holidays, and other allowed absences; and insurance, retirement, and health benefits. To successfully administer a benefits program, HRM professionals need to understand tax incentives, retirement investment plans, and purchasing power derived from a large base of employees Pearls of Perspicacity: Proven download pdf Pearls of Perspicacity: Proven Wisdom to. Thus the universe has no end or edge, and so nothing is outside the universe or "beyond its edge". For the size of the universe, humans only know a lower bound -- namely, the size of the observable universe -- and probably cannot know an upper bound, although it is often assumed to be finite 68 steps: How to Easily download here http://6on6.playground.wedeking.org/?freebooks/68-steps-how-to-easily-achieve-a-magnificent-life-finance-social-wellness-joy. Compensation and benefits are usually included in the terms of the contract; and include annual salary, hourly wage, or the flat fee, as well as any health insurance or retirement benefits , cited: Delivering Business-Critical read epub read epub. Social Perceptiveness - Being aware of others' reactions and understanding why they react as they do Pulse: The New Science of read for free Pulse: The New Science of Harnessing. This was then recognized by the United Nations. As the above given information clearly states that in the olden days when people were not treated as humans but as an asset of the company there were many socialist parties those who wanted there to be a system by which these human beings could have their own problems and their own matters solved epub.
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UNIVERSITY OF IOWA PA/ARNP HOSPITALIST in IOWA CITY, Iowa
PA/ARNP HOSPITALISTRequisition # 71643Position BasicsAdvertising Ends on:Friday, October 13th, 2017Advertising Started on:Monday, September 11th, 2017Organization:Carver College of MedicineDepartment:Orthopaedics and RehabilitationSalarySalary:$100,000.00 to CommensuratePay Grade:7AJob DetailsPercent Time:100%Type of PositionRegular: A position which is considered essential for the effective long-term operation of the university. Persons appointed to this position will receive the privileges and benefits associated with regular employment status.Duties:In collaboration with the spine surgeons, multidisciplinary team, and Orthopaedic Department Administrator, coordinates the continual monitoring and evaluation of effectiveness and operations of the Spine Service. Assist with identification and implementation of process improvement projects and service line advancements in order to improve patient outcomes and decrease complications. Assist with implementation of methods to capture appropriate billing/coding documentation in the EMR.Education Required:ARNP: A Master's degree is required. A Master's degree in Nursing is preferred. If Master's degree is in a related field, a Baccalaureate degree in nursing is required.PA: Must comply with registration and licensure requirements as specified by the Iowa Board of Physician Assistant Examiners which include graduation from an accredited PA educational program and/or certification by the NCCPA.Experience Required:3-5 years of patient care experience is required.Demonstrated experience and knowledge of orthopaedic patient care are required.Experience working in surgical inpatient or operating room setting is required.Excellent written and verbal communication skills are required.Demonstrated experience working effectively in a diverse environment or promoting a diverse environment is required.Certifications:ARNP: Current license to practice nursing in Iowa is required. Current certification in specialty area and licensure as ARNP in state of Iowa is required.PA: Must comply with registration and licensure requirements as specified by the Iowa Board of Physician Assistant Examiners which include graduation from an accredited PA educational program and/or certification by the NCCPA.Online Application OptionsApplication Type:This job requires basic applicant information, plus a resume. Your resume should not be sent to the contact below. The resume will be attached during the Online Application Process.Cover Letter:A plain text cover letter is required.To start the Online Application process for this position, click the "Apply for This Position" button located below the Contact Information.Contact InformationAttention: Your resume should not be sent to the contact below. The resume will be attached during the Online Application Process.Contact:Sarah M Burnett - email@example.com and RehabPOMERANTZ FAMILY PAVILION720 MELROSE AVE01167-A PFPIOWA CITY, IA 52242Phone: 319-356-7121For information about applying for P&S positions, please go toProfessional and Scientific Position Application ProceduresThe qualifications for the positions are listed in the job advertisement.Equivalent combinations of education and related work experience may be considered. The salary listed indicates the minimum for the pay grade to a level commensuratewith qualifications and experience. To review P&S or SEIU pay schedules or for acomplete classification description go to theUniversity of Iowa Compensation andClassification web site.The University of Iowa is an equal opportunity/affirmative action employer. All qualified applicants are encouraged to apply and will receive consideration for employment free from discrimination on the basis of race, creed, color, national origin, age, sex, pregnancy, sexual orientation, gender identity, genetic information, religion, associational preference, status as a qualified individual with a disability, or status as a protected veteran. The Universi

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African Sun profits jump 134pc
By Enacy Mapakame
HARARE- Hospitality group, African Sun Limited is upbeat on maintaining a growth trajectory into the second half of the year after reporting a 134 percent jump in profit for the six months to June 30, 2017.
The growth will be driven by an anticipated influx in tourist arrivals into the country, especially the Victoria Falls coupled with a boom in conferencing.The second half of the year also covers the festive season which constitutes the peak period for the hospitality industry.
Already, Victoria Falls has seen arrivals improve by 40 percent following completion of the resort town's airport expansion.
"The remaining part of the year falls into our peak trading system," said group chairman Mr Herbert Nkala in a statement accompanying financial results.
"We expect conferencing and international market business to bolster performance, particularly in our Victoria Falls properties where inward foreign arrivals have been on an increase," he said.
Meanwhile, African Sun posted a profit of $0,19 million for the half year to June compared to a loss of $0,56 million in the same period last year.Management attributed the turnaround to the growth in revenue, which jumped 17 percent to $21,01 million.
The revenue growth was as a result of an 8 percentage points increase in occupancy to 45 percent from 37 percent.Revenue per available room increased by 14 percent to $40 from $35 achieved in the prior year.
Occupancy growth was also supported by a strong performance from all markets with local, international and regional rooms sold increasing by 21 percent, 33 percent and 2 percent respectively.
Mr Nkala said the group would continue focusing on revenue generation and cost reduction initiatives to navigate the challenging business environment.


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The spontaneous electrical activity of uterine smooth muscle was rather variable when acute observations were made. Therefore, a series of chronic experiments was performed with implanted electrodes to monitor a group of myometrial cells under different physiological conditions for periods up to eight weeks. The results showed that consistent behavior of myometrial cells could be observed provided similar hormonal status was maintained. Action potentials were rare or absent in myometrium of oophorectomized animals but were caused to appear by estrogen. In the pregnant uterus, action potentials increased both in amplitude and frequency of discharge as parturition approached, reached a peak at that time, and then declined in the postpartum days. The results indicated that in estrogen treatment and in parturition activities of myometrial cells were more synchronous. There was suggestive evidence that there were central impulses initiating activity in the pregnant uterus, and that the responses of the myometrium were affected by the local conditions.
- Copyright © 1959 by American Physiological Society


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It's been 11 years since I ran a marathon, so my recollections are a bit foggy for comparison, but here goes. I will give my personal account of MY experience in this race tomorrow.
On the weekend of October 20-21 you have literally dozens of choices for marathons. I'm not even going to try to list them all, but I will tell you about the best one. The Waddell and Reed Kansas City Marathon.
The race, by most standards for marathons, is small. The final count was 6,200 participants for the full marathon, half and 5K combined, with the majority running the half (I think I heard about 1200 for the marathon). The men's winner was a FIRST TIME MARATHONER!!! He finished with a time around 2:30:00. The women's winner was a local high school vice principal who finished with a time of about 3:00:00. While neither was a world class time, I think it really adds to the charm and interest of this race. It is definitely a bigger race that anyone can win. I think it makes it even more fun.
This course is obviously designed to showcase some of the best sights in Kansas City. I'm not going to go turn by turn, but just give what I felt were the highlights. This is a BEAUTIFUL course that covers everything from the concrete jungles of downtown Kansas City to the spectacular mansions around mission hills. It is obvious that the designers purposefully planned the course to go by every major fountain in the city, and it was a great show.
We started off facing downtown from just outside the Crown Center (think Hallmark Cards, big mall, ice skating and historic train depots), taking the gun just as the sun began to rise. After a brief choke point through the start/finish gate (the organizers didn't expect as many runners as they got. Here's a hint guys… Make the start finish go ALL THE WAY across the street next year…!) which was still very brief, we were off and running on the nice wide city streets within 100 yards of the start. The first two miles of the course take you right at downtown and then turn to go through one of the many history rich sections of Kansas City called the Freighthouse District. You skim it the first time (but eventually finish here) and then reverse course and head back within a block of the finish line (great for spectators!!) before turning past Union Station and then heading up the first big hill. I, personally thought this was the toughest one because, although short, it was a bit steeper. But your reward at the top is a loop through a beautiful World War I Memorial with the skyline in the background. A jazzy, bluesy band greeted us at the apex of our loop. Then it was on past the first water stop (about every 2 miles) and up a little hill and then down the backside into a fun rolling section leading you into Westport. Shops, bars, a couple of more good (albeit blurry eyed to be playing at that time of the morning) bands and lots more fountains and architecture to look at as you cruise through this section and find your rhythm.
You head out of Westport and into another landmark, the Plaza. High end shops, lots more fountains, a few more bands and flat to rolling roads mark this section. One of Kansas City's biggest runs, the Trolley Run (about 10,000 runners) happens on this section of the course. Other sights include great old houses, parks and creeks (did I mention the fountains?). Make sure to cruise through here and not push it too hard because the next section is the longest hill on the course.
As you come down Ward Parkway out of the Plaza, you make a right on Sunset Drive. The houses here are huge. Climb through these mansions, under enormous oak and maple trees as you climb for about 1 mile. The homeowners, much like the bands, greet you with bleary eyed smiles and words of encouragement as you pass. No bands here though… heh! Once you get to the top, you are greeted by Loose Park. Just know that when you see it, you are done with hills!! In fact, the next 7 miles (miles 13.1 to 20) are mostly flat to downhill. A great time to recover and soak in the views. Beautiful neighborhoods, parks, creeks, fountains and a few more bands really help get you through that mile 15-20 stretch that can be so hard. This section finishes with an out and back 4 mile section that takes you to, you guessed it, another fountain. Another unusual thing that I liked about this course was the number of times you were close enough to see the other runners. In several places you ran out and back or very close loops and it just felt like there were runners everywhere. Since I did most of my training alone, seeing so many runners was comforting (especially around mile 20… it was nice to see that everyone was struggling a bit…!).
At about mile 21 you start to climb a bit but it is very gradual. Up past Hyde Park you get your first view of the downtown area again, which will give you a big surge of energy since that is where you finish! The road flattens out as you make a quick loop through 18th and Vine District. Lots of jazz and blues history here (and two REALLY good bands!!!) and then flat to downhill to the final turn onto Baltimore and a nice downhill finish into the Freighthouse district!
The finish line festival was well stocked with food and another Kansas City favorite, Boulevard Brewing Company (I recommend the Wheat!), had another good band and lots of aid for weary runners. I hung around for as long as my legs would let me and then walked the two blocks back to my hotel and collapsed, having just successfully bagged my first marathon since my "re-birth" as a runner.
Pros and cons:
- The course! Gorgeous and just challenging enough to let you know who's boss.
- The volunteers. Not thousands, but well stocked enough to make for a good, safe race. They were very supportive and always smiling.
- The organization. Packet pickup was easy and everybody knew the system.
- Race direction. Why? Start time was within a minute of schedule. Ya gotta love that.
- Pacers. They were good, positive and helpful throughout the race… although no one should be laughing and chatting ALL THE WAY through the race… They made it look too easy…
- The expo. It was dead. And the vendors (with a few notable exceptions) were too. They just seemed like they either didn't want to be there or didn't know what to do. We went in again an hour before the expo was "supposed" to close and just about everyone was already gone or already packing.
- Sound system. I think the race people may need to upgrade their sound system to something that can cover a wider area. We couldn't here much until we were called to the line because the porta potties were a bit too far from the starting line and the P.A. system was too weak to get noise out that far.
- Volunteers. Don't get me wrong. The volunteers they had were awesome. All of the water stations were well stocked with stuff and people. But there were some spots on the course where they were uncomfortably thin. One elderly woman with an orange vest and a stop sign is not enough to keep irritated drivers from pushing through the course. As the race grows, this is something the officials are going to have to work on.
If you weren't there, you missed a REALLY great run. I couldn't have asked for anything better. If you weren't there and you are a fast runner, you missed your chance at an easy $1000. So when next marathon season comes around and you are looking for a good one, keep this one in mind. Great scenery, great food, a challenging course, fun volunteers and a city that WANTS you to be there. Did I mention they have fountains?


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The stroke-prone spontaneously hypertensive rat (SHRSP) is a model of heritable hypertension-associated cerebrovascular injury. This study sought to compare SHRSP to the stroke-resistant SHR strain to identify genes and protein pathways whose expression and/or function was significantly altered between the strains prior to the onset of stroke. Cerebral cortex gene expression profiles from male SHRSPs and matched SHRs were examined by Affymetrix microarray analysis. mRNAs encoding the brain-derived neurotrophic factor receptor (TrkB) and multiple kinases of the MAPK/AKT signaling pathways, including JNK2, AKT2, and PI3K, were differentially expressed between SHRSP and SHR. Because these data suggest altered function in pathways involving MAP and AKT kinase activity, we performed Western blot using phosphorylation state-specific antibodies to characterize activity of MAP kinase and PI3K/AKT pathways. Changes in the levels of the phosphorylated forms of these kinases paralleled the changes in transcript levels observed between the strains. Two-dimensional gel electrophoresis and peptide fragment mass fingerprinting were used to identify altered protein substrates of these kinases. Protein profiling of kinase substrates further supported the notion of perturbed kinase-mediated signaling in SHRSP and identified adenylyl cyclase associated protein 2, TOAD-64, propionyl CoA carboxylase, APG-1, and valosin-containing protein as kinase targets whose phosphorylation state is altered between these strains. Altered gene and protein expression patterns in SHRSP are consistent with increased vulnerability of this strain to cerebrovascular injury.
- microarray
- kinases
susceptibility to stroke in humans has a complex etiology involving both environmental and genetic factors (6, 14). Although a few rare Mendelian syndromes producing stroke have been well characterized, genetic susceptibility to the common form of this disorder is polygenic (22). Little is known, however, about the genes involved or the pathogenetic mechanism these genes direct and which evolves prior to the occurrence of stroke. In particular, very little information has been gained on the antecedent events unfolding in brain tissue that reflect the progression from genetic susceptibility to frank disease.
The stroke-prone spontaneously hypertensive rat (SHRSP) has been developed by selective breeding from the SHR strain. While SHR is resistant to stroke, SHRSP develops cerebrovascular injury at high frequency (21). The genetic mechanism of stroke in SHRSP involves multiple genes. Mapping studies have begun to define three chromosomal loci that may harbor genes responsible for about 28% of the variation in stroke risk between SHRSP and SHR (23). However, little progress has been made in further identifying the localized susceptibility genes or understanding how they initiate or control the disease process.
In the present study we have combined global gene expression and proteomic approaches to examine cerebral tissue prior to the occurrence of stroke in SHRSP. By contrasting SHRSP with age- and sex-matched SHR animals, we have sought to shed light on potential pathogenetic mechanisms and the underlying genes that contribute to this model of polygenic stroke susceptibility. Our results point to alterations in several related protein kinase-mediated signaling pathways that may be relevant to the pathogenesis of stroke in SHRSP and are of potential significance in human stroke.
Animals, sample collection, and RNA extraction.
Studies were performed on six male SHRSPs and six male SHRs from breeding colonies maintained by the investigators. These are the same substrains used by others in genetic linkage studies to identify chromosomal regions contributing to stroke (23). Age-matched male rats from both strains were fed a standard rat chow and water ad libitum until age 8 wk, and both strains subsequently received a "stroke-permissive" diet (18.7% protein, 0.63% potassium, 0.37% sodium) and 1% NaCl drinking solution. Animals were killed at age 12 wk. They did not exhibit any evidence of stroke after behavioral assessment (29) and histological examination (3) at the time of sampling. Cerebral cortices were dissected and immediately frozen in liquid nitrogen. RNA was extracted using the method of Chomczynski and Sacchi (7) adapted in the RNAzol protocol (TelTest, Friendswood, TX). Each animal was used to generate a single RNA sample and there was no sample pooling.
Microarray experiments, data analysis, and validation.
Microarray analysis was performed as described previously (19). Briefly, 10 μg total RNA from 12 independent samples (6 SHRSPs, 6 SHRs) was used to synthesize cDNA, which was then used as a template to generate biotinylated cRNA. cRNA was fragmented and hybridized to Test2 microarrays to verify quality and quantity of the samples. Each sample was then hybridized to a RG-U34A array (Affymetrix, Santa Clara, CA). After hybridization, each array was washed and scanned, and fluorescence values were obtained using the Affymetrix Microarray Suite version 5.0 software. Data were analyzed using the method develop by Tusher at al. (28) implemented in the SAM ("significance analysis of microarrays") package. Expression values were normalized within sample, to the median of all measurements in that sample, and within gene, to the median of all measurements for that gene. Analyses were performed on the log scale. The mixture model approach of Tusher et al. (28) takes full advantage of the existence of replicated data to estimate the null distribution of the test statistic directly from the data. For each gene, the "test statistic" d(i), or relative difference in gene expression is given by where x̄SP(i) and x̄SR(i) are defined as the average levels of expression for gene (i) in SHRSPs and SHRs, respectively; s(i) is the standard deviation of repeated measurements; s0 is a constant computed to minimize the coefficient of variation of d(i). The expected value of the test statistic dE(i) was calculated after performing a set of 500 permutations of the vector of gene expression measurements over the samples. Genes were considered differentially expressed if d(i) − dE(i) was greater than a threshold Δ. The threshold Δ was chosen so that the false discovery rate (FDR) associated with the identified genes is less than or equal to 5% (i.e., among the genes called significant, 5% or less are false positive).
To compare gene expression using a different methodology, we examined expression of a subset of genes using the 5′ nuclease (TaqMan) RT-PCR assay. The same RNA samples as those used in the microarray experiments were used in the validation experiments. Primer and probe sequences are available as Supplemental Table 1, available at the Physiological Genomics web site.1
Western blot analyses.
Cortical tissue samples from the same animals as those used in the microarray experiments were Dounce-homogenized in 3 volumes hypotonic lysis buffer containing (in mM) 10 Tris, 10 NaF, 10 sodium pyrophosphate, 10 β-glycerophosphate, 1 Na3VO4, 1 EDTA, adjusted to pH 7.4 and containing 1% Igepal CA-630 nonionic detergent. Following determination of protein concentration by Coomassie dye assay (Pierce), sample aliquots were adjusted to 2 μg/μl and boiled after addition of SDS sample buffer. Samples were resolved on 6–15% SDS-PAGE gels (20 μg/lane), transferred to PVDF membranes, blocked in 5% nonfat dry milk in Tris-buffered saline with 0.05% Tween-20 (TTBS) followed by overnight incubation in primary antibody diluted in 1% milk-TTBS. Blots were rinsed in TTBS and incubated in the appropriate horseradish peroxidase-labeled secondary antibody, either goat anti-rabbit or anti-mouse (Cell Signaling Technology, Beverly, MA), at 1/20,000 in 1% milk-TTBS for 1 h. After rinse, blots were incubated in enhanced chemiluminescent substrate (Pierce SuperSignal West Pico) and exposed to film (Kodak BioMax ML). The following phosphorylation state-specific primary antibodies to specific kinases were used (all from Cell Signaling Technology): extracellular regulated kinase dually phosphorylated at residues Thr202 and Tyr204 (ppERK, 1/2,000); 90-kDa ribosomal S6 kinase phosphorylated on Ser380 (pRSK, 1/1,000); Jun terminal kinase phosphorylated at residues Thr183 and Tyr185 (pJNK, 1/1,000); phospho-p38 (1/500); AKT kinases phosphorylated on Ser473 (pAKT, 1/1,000). We also used antibodies (all from Cell Signaling Technology) to conserved phosphorylation motifs to detect changes in phosphorylation state of substrates of AKT (antibody detects phospho-Ser/Thr preceded by Lys/Arg at positions −5 and −3), 3-phosphoinositide-dependent kinase 1/PDK1 (antibody detects T* FCGT motifs), and MAPK/CDK family of proline-directed kinases (antibody detects phospho-Thr followed by Pro, pTP motifs) (27). Welch t-tests for equality of means were used to determine whether phosphorylated protein amounts differed among the two groups of rats.
Two-dimensional gel electrophoresis and mass-spectrometry identification of pTP substrates.
Samples were fractionated on an anion-exchange column (High Q, Bio-Rad). Fractions eluted with an increasing NaCl gradient (0–1,000 mM) were precipitated in 10 vol of acetone (−20°C overnight). The pellets were sonicated in 9 M urea, diluted to a final concentration of 1 μg/μl (9 M urea, 5× SDS buffer), and boiled. We loaded 20 μg per lane (6–15% gradient gels), and Western blotting with the pTP antibody was performed as described above. Fractions that were enriched for pTP-immunoreactive bands were precipitated in acetone and resuspended in two-dimensional (2D) electrophoresis buffer (Bio-Rad Ready-Prep 2). Initially, 500 μg was loaded onto a 7-cm wide-range (pH 3–10) IPG strip (Bio-Rad) for isoelectric focusing (10,000 V-h, 4,000 V). After focusing, strips were equilibrated in sample buffer (9 M urea, 150 mM Tris, pH 8.8, 3% SDS) containing first 1% DTT for 10 min followed by 2.5% iodoacetamide for 10 min. Samples were placed on 6–15% gradient gels, and Western blotting for pTP was performed. For protein identification, 3 mg protein was loaded onto 17-cm narrow-range (pH 5–8) IPG strips, focused (30,000 V-h, 10,000 V), and resolved by second-dimension SDS-PAGE, and Western blots were probed for pTP. Replicate gels were aligned with the immunoblots, and pTP-immunoreactive spots were excised and subjected to peptide fragment mass analysis by electrospray mass spectrometry (PerkinElmer/SCIEX API 3000) after in-gel tryptic digestion.
Gene expression array studies.
We identified 129 probes, representing 117 unique genes or expressed sequence tags (ESTs), differentially expressed in the cerebral cortex of 12-wk-old SHRSPs and SHRs on a stroke-permissive diet (see Supplemental Table 2). The identified genes had known functions in the brain or central nervous system and belonged to major functional classes such as neurotransmission, intracellular signaling, cell proliferation, metabolism, and RNA transcription (Supplemental Table 2). The transcription factor JunD exhibited the greatest difference in expression among the genes identified (∼15-fold lower in SHRSP vs. SHR). The gene encoding the atrial natriuretic peptide (ANP) was expressed at significantly lower levels in SHRSP compared with SHR (average difference was −3.2-fold across 2 independent probe sets). This is in agreement with earlier reports describing a threefold lower expression of ANP mRNA in the brain of SHRSP compared with SHR (17, 24).
The chromosomal location of the differentially expressed genes was determined using map information from the rat genome assembly (UCSC version 2) and comparative maps of the human, mouse, and rat genomes. Genes mapping to the chromosomal regions previously identified as containing quantitative trait loci (QTLs) influencing stroke in this animal model (23) are shown in Supplemental Table 2. These transcripts were ANP, the potassium voltage-gated channel Kcnab2, and CaMK2 inhibitor α, which map to the QTL on chromosome 5; casein kinase 2, which maps to the QTL on chromosome 3; and Doc2A and sulfotransferase A1, which map to the QTL on chromosome 1.
Multiple genes encoding members of the serine/threonine kinases superfamily were found differentially expressed between SHRSP and SHR. Among those with a higher expression in SHRSP were the catalytic β-subunit of cAMP-dependent protein kinase (1.5-fold), the AKT2 kinase (1.9-fold, averaged over two probe sets; P = 0.01), the Janus protein tyrosine kinase 2 (2.3-fold), the JNK2 isoform (1.3-fold), and the protein kinase C α-subunit (1.9-fold). The cGMP-dependent protein kinase 2, the casein kinase 2α (CK2), and the glycogen synthase kinase 3β (GSK3) had a significantly lower expression in the SHRSP compared with SHR (−1.5-fold, −1.6-fold, and −1.8-fold, respectively). Known regulators of these serine/threonine kinases were also found differentially expressed between the two strains. For example, expression of DUSP6, the dual specificity phosphatase that selectively inactivates ERK2, was significantly decreased in SHRSP (1.4-fold, average of two probes). Similarly, the phosphoinositide 3-kinase (PI3K) p85, which stimulates the activity of the AKT kinases, also had a significantly lower expression in SHRSP (−3-fold, average of two probes). Expression of Map4k1, a specific regulator of the JNK pathway, was significantly lower in SHRSP compared with SHR (−9-fold).
The neurotrophin receptor protein tyrosine kinase, TrkB, was also found significantly differentially expressed. The TrkB gene encodes two major isoforms by alternative splicing: the full-length mRNA (FL-TrkB) generates a 145-kDa transmembrane tyrosine kinase and neurotrophin receptor. An alternatively spliced mRNA generates a truncated form of the receptor lacking the intracellular tyrosine kinase domain (95 kDa). Two probe sets, specific to each isoform, were present on the array, but the differences in expression between SHRSP and SHR for these probes were in the opposite direction. The FL mRNA had a 1.4-fold lower expression in SHRSP compared with SHR, while the truncated form was expressed at a higher level of ∼2-fold (not significant).
Independent verification of array findings.
To confirm the microarray results, we measured the relative expression of a subset of the identified genes by the TaqMan assay. We observed good concordance between the array data and the TaqMan data for the direction of changes in expression between the strains (Fig. 1). However, estimates of the magnitude of the expression differences between SHRSP and SHR were consistently lower by the TaqMan assay. This assay was unable to detect small but consistent differences in gene expression that were consistently detected by multiple independent probe sets on the array (e.g., stearoyl CoA desaturase, Scd2, 3 independent probe sets, detected fold change for each set: 1.4, 1.5, and 1.5).
Western blot analysis to follow-up altered TrkB transcript abundance.
In light of the evidence for perturbed gene expression of components of protein kinase-mediated signaling pathways in the SHRSP and the important role of such pathways in susceptibility and response to cerebral ischemia, we sought to determine whether the observed differences might be translated at the level of protein expression and/or function. A Western blot analysis of the TrkB receptor was carried out using a monoclonal antibody that allows determination of both the full-length and the 95-kDa isoforms of the protein (BD Biosciences). As in the gene expression analyses, we observed a significant decrease in protein abundance of the FL-TrkB receptor in SHRSP compared with SHR (fold change = −2.3, P = 0.005), whereas the level of the TrkB 95-kDa isoform was significantly increased (fold change = 2.6, P = 0.003) (Fig. 2).
Protein kinase activation in SHRSP cerebral cortex.
We next performed a Western blot analysis with phosphoprotein-specific antibodies to activated kinases to determine whether the observed differences in gene expression of several serine/threonine kinases were accompanied by differences in phosphorylation level of these proteins. Dually phosphorylated ERK protein abundance was significantly increased (1.8-fold) in SHRSP compared with SHR (P = 0.002) (Fig. 3A). An antibody to the total ERK protein did not, however, detect any significant difference in the amount of either total ERK1 or ERK2 between the two strains (P = 0.80 and 0.58, respectively; not shown). Increased level in SHRSP cortex of pRSK (Ser380) (3.7-fold; P = 0.005), a known substrate of ERK, is also consistent with the increase in SHRSP ERK activity, as measured by its level of phosphorylation (Fig. 3B). We also investigated whether JNK phosphorylation level was significantly different between the two strains. As shown in Fig. 3C, there was a significant increase (∼1.7-fold) in pJNK in SHRSP (P = 0.03). Total JNK protein was also significantly increased in SHRSP (JNK1, 1.7-fold, P = 0.01; JNK2, 1.3-fold, P = 0.04) (not shown). We were unable to detect p38, another member of the mitogen-activated protein kinase (MAPK) superfamily. This is likely due to its predominantly nuclear location. Gene expression data also suggested differences between SHRSP and SHR in the AKT signaling pathways. We observed a small, but significant increase in pAKT in SHRSP compared with SHR (1.3-fold, P = 0.01) (Fig. 3D), whereas no difference between the strains was detected in the levels of total AKT protein (P = 0.80) (not shown).
Altered kinase substrate phosphorylation in SHRSP.
To further corroborate our hypothesis that MAPK- and AKT kinase-mediated signaling is perturbed in SHRSP cortex prior to the onset of stroke, we next screened cortical tissues from SHRSP and SHR for differences in abundance of phosphorylated substrates of these kinases using phosphorylation state-specific antibodies. ERK, JNK, and GSK3 belong to the family of proline-directed serine/threonine kinases, which phosphorylate proteins at threonine or serine residues followed by a proline residue. By Western blot analysis using an antibody that specifically recognizes phospho-threonine-proline (pTP) motifs, we detected the presence of several phosphorylated substrates whose level of expression is increased in cortical tissue from SHRSP compared with SHR (Fig. 4A). These data extend and confirm those which show that activity of proline-directed kinases is increased in SHRSP, as increased substrate phosphorylation is likely a functional consequence of increased ERK/MAPK, JNK, and/or other proline-directed kinase activity.
We next determined whether the altered expression of genes belonging to the AKT pathway observed in the microarray analyses was functionally relevant, assessed by examining the abundance of substrates phosphorylated by AKT and PDK1. We observed significant differences in abundance of AKT-phosphorylated substrates between SHRSP and SHR (Fig. 4B). Similarly, we showed significant differences in abundance of phosphorylated PDK1 substrates between the two strains (Fig. 4C). These data further demonstrate evidence of perturbations in multiple kinase-mediated signaling pathways in SHRSP prior to the onset of stroke.
Identification of altered kinase substrates in SHRSP.
Identification of specific targets of activated proline-directed and AKT kinases may provide further information about the pathophysiological consequences of perturbations in kinase-mediated signaling in SHRSP. We were able to identify by mass spectrometry several kinase-regulated substrates. Anion-exchange chromatography was first used to enrich for the pTP substrates, and gel electrophoresis was used to determine which purified proteins were of differential abundance. Four proteins which show significant difference in abundance between SHRSP and SHR in Western blotting with pTP antibody (Fig. 5A, proteins indicated with arrows) appear in the 300 mM NaCl anion-exchange eluate (Fig. 5B). The 300 mM NaCl fraction was then resolved by 2D electrophoresis (Fig. 5C). The corresponding spots on the gel were punched, trypsinized, and analyzed by peptide mass fingerprinting using mass spectrometry. Three of these proteins could be identified: adenylyl cyclase associated protein 2, TOAD-64, and propionyl CoA carboxylase. The band from the 600 mM fraction (Fig. 5B) was also resolved by 2D electrophoresis and was shown to be two separate proteins of similar mass, APG-1 and valosin-containing protein.
To improve our understanding of the molecular mechanisms involved in the SHRSP predisposition to spontaneous brain damage, we have carried out microarray gene expression profiling and functional proteomic analyses of cortical tissue from age-matched SHRSP and SHR prior to stroke occurrence. The TrkB receptor is the high-affinity receptor for two neurotrophins: brain-derived neurotrophic factor (BDNF) and neurotrophin 4/5. Both are important promoters of neuronal survival, growth, and differentiation and regulate synaptic transmission. Evidence is accumulating for a crucial role of TrkB in protection from neuronal damage after ischemia. In adult rats subjected to permanent middle cerebral artery (MCA) occlusion, both TrkB mRNA and expression of the FL-TrkB protein were significantly increased in cortical neurons distant from the infarct (2, 10). This increase was associated with a decreased neuronal cell death in the penumbra area and appeared to be mediated through elevation in BDNF levels (10). Similar findings were obtained in a gerbil model of global ischemia (9). In the present studies we found that TrkB was significantly differentially expressed between the SHRSP and SHR strains.
The TrkB receptor is expressed as multiple isoforms produced by alternative mRNA splicing. Both the FL and the truncated forms of the receptor were differentially expressed between SHRSP and SHR, but in the opposite direction. The FL receptor was significantly downregulated, and the truncated form was significantly upregulated in SHRSP. The FL form of the receptor possesses a tyrosine kinase domain that is lacking in the truncated form. Although the intracellular signaling mechanisms used by FL-TrkB to promote neurotrophic actions are well characterized, the physiological functions of the truncated receptors remain poorly understood. Recent data have suggested that they inhibit FL-TrkB-mediated cell survival by acting as a dominant-negative receptor either by preventing FL-TrkB activation or by trapping the ligand and preventing its interaction with FL-TrkB (8, 12). The role of the truncated form of the receptor in TrkB-mediated neuroprotection from ischemic injury has been examined in a recent study by Saarelainen et al. (25), which reports that mice overexpressing truncated TrkB receptor in neurons suffer much greater cortical damage after focal cerebral ischemia than their wild-type littermates. These data are consistent with the hypothesis that aberrations in TrkB expression may promote SHRSP vulnerability to cerebrovascular lesions.
The TrkB receptor promotes neurotrophic events through actions mediated by at least three types of multifunctional, highly conserved serine/threonine protein kinases: CaMK2, CK2, and MAPKs (4). All three pathways were found significantly differentially expressed between SHRSP and SHR. More recently, the PI3K/AKT pathway was also implicated in Trk receptor-mediated neurotrophins action (16). We found a significant increase in AKT2 gene expression and a significant decrease in PI3K gene expression in SHRSP. Moreover, a known target of the AKT signaling pathway, the GSK3, was also significantly differentially expressed between the two strains. Perturbations in expression of these kinases may represent a functional consequence of altered TrkB expression; alternatively, they may contribute to SHRSP stroke vulnerability by mechanisms independent from TrkB.
MAPKs regulate gene expression and protein synthesis. There is growing evidence that MAPKs play pivotal roles in the regulation of neuronal survival and apoptosis. Alessandrini et al. (1) reported that inhibition of the MAPK/ERK pathway affords significant neuroprotection from brain injury resulting from MCA occlusion. More recently, inhibition of ERK2 phosphorylation has been directly correlated with inhibition of neuronal death in an animal model of ischemia/reperfusion injury (20). We report evidence for an activation of the ERK and JNK pathways in the cortex of SHRSP prior to the onset of stroke. JNK gene expression and activated ERK and JNK protein abundance were increased in SHRSP compared with age-matched SHR. The increased ERK1/2 protein activity, as measured by its level of phosphorylation, observed in the cortex of SHRSP is consistent with the enhanced vulnerability of this model to cerebrovascular injury.
Previous studies have reported increased ERK and JNK activities in the left ventricle and aorta of SHRSP compared with the normotensive closely related strain, WKY, and have proposed a role of these kinases in the development of hypertensive cardiovascular disease (13, 18). The effect of increased ERK and JNK activities on the development of left ventricular hypertrophy in SHRSP was shown to be independent from any effect on blood pressure and appeared to be mediated by distinct mechanisms. Although further studies are needed to elucidate the mechanisms and significance of the increase in cortical ERK and JNK in SHRSP, this is, to our knowledge, the first report suggesting a perturbed MAPK-mediated signaling in the brain of SHRSP prior to stroke occurrence.
JunD, a member of the Jun family of transcription factors, is a known target of the MAPK cascade and was differentially expressed between SHRSP and SHR both consistently and at high levels. The significance of this result is unclear. A previous study examining JunD expression in the rat hippocampus after transient ischemia has shown that JunD induction after ischemia represents a protective reaction to ischemic stress (15). Thus the reduced levels of JunD expression in SHRSP corroborate our finding of patterns of gene expression in SHRSP consistent with an increased vulnerability of this strain to cerebrovascular lesions.
Both SHRSP and SHR develop hypertension at early age but by 10 wk of age, SHRSPs blood pressure levels are greater than SHRs. Our animals were sampled at 12 wk of age. We, therefore, cannot exclude the possibility that some observed differences in gene expression may be due to differences in blood pressure between the two strains. It is well established that high levels of blood pressure contribute to expression of the stroke phenotype in the SHRSP and that lowering of blood pressure greatly reduces the occurrence of stroke in these animals (5). It is, therefore, likely that among the genetic factors that confer stroke susceptibility to the SHRSP, some will contribute to stroke via effects on blood pressure. Identification and characterization of such genes will be important to the understanding of the mechanisms underlying hypertension-associated stroke susceptibility in this model.
The goal of studies employing genome-scale gene expression profiling to genetic disease susceptibility is to shed light on a broad range of systems that may be altered in the disease model and to make relevant correlations that may indicate fruitful targets for more focused investigation. As such, this study has provided the first evidence that gene and protein expression levels of the TrkB receptor and protein activity of multiple kinases mediating its signaling are altered in the cortex of SHRSP prior to the onset of stroke. Additional gene expression profiling experiments by others may verify the data and inferred hypothesis reported here (26). The present study does not permit to firmly establish a causal relationship between changes in gene expression, functional alterations in the corresponding protein pathways, and stroke susceptibility. However, the hypothesis that the observed perturbations are consistent with an enhanced vulnerability of the SHRSP to neuronal injury and thus may play an important role in the pathophysiology of cerebrovascular lesions in this animal model is intriguing and provides the basis for further targeted investigations (11). In particular, we have begun to identify and characterize sequence variation in genes whose expression was significantly different between the two strains (11). Resequencing of the TrkB mRNA sequence has already identified two variants: a synonymous A to G substitution at position 1450, and a C to T substitution at position 1224 which results in an amino acid change from an alanine to a valine in the second cysteine-cluster domain of the receptor involved in ligand binding (not shown). Future analyses will determine whether this mutation cosegregates with stroke and stroke-related phenotypes in crossbred animals to assess its role in disease etiology.
This research is supported by National Institutes of Health Grants RO1-NS-41466 and RO1-HL-69126 (to M. Fornage).
We thank Emily Wang and Vance Wise for expert technical assistance.
↵1 The Supplementary Material for this article (Table 1, which is a list of the primer and probe sequences, as well as Supplemental Table 2, which is a comprehensive list of mRNA transcripts showing a differential expression in the cerebral cortex of age- and gender-matched SHRSP as compared to SHR) is available online at http://physiolgenomics.physiology.org/cgi/content/full/00020.2003/DC1.
Article published online before print. See web site for date of publication (http://physiolgenomics.physiology.org).
Address for reprint requests and other correspondence: M. Fornage, Institute of Molecular Medicine, Univ. of Texas Health Science Center-Houston, 2121 W. Holcombe Blvd., Houston, TX 77030 (E-mail: myriam.fornage{at}uth.tmc.edu).
- Copyright © 2003 the American Physiological Society


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NewsNovember 28 2012
Thrift Store accepting holiday donations
Posted by Lucy
With the holidays near, Teresa Hanson, Presho Thrift Store n More board member, said donations to the food pantry, along with other holiday items such as wrapping paper, tape, decorations, gently used toys, winter items and household items are needed.
The Thrift Store will hold a kids shopping day Sat., Dec. 8 and provide holiday food baskets and certificates to those in need prior to Christmas.
- Kennebec Elementary take a ride
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HARIPUR: The wedding ceremony of Pakistan's former express bowler Shoaib Akhtar was held in Haripur district of Khyber Pakhtunkhwa on 23 June, DawnNews reported on Wednesday.
The report further said that during the nikkah ceremony the 'haq mehr' was set at Rs500,000 adding that the newly-wed couple had departed from Haripur after the 'rukhsati' event.
The low-key affair was attended by family members and close friends of the groom and 20-year-old bride.
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Comments (58) Closed
Heartiest congratulations to the Rawalpindi Express for getting married and embarking upon a new journey in life. Best of luck to him and his family. Wishing Shoaib Akhtar a happy, prosperous and successful married life.
Congratulations to Shoaib !
39 yrs male marries 20 yrs old girl. Stay Classy Shoaib ..
I think the video said that the bride is 20 years younger than the groom, so please correct the news
Good.may be we can see another sohaib in next 20 years
He married someone half his age? Thats weird. Last month when there was rumour about this marriage, he denied upfront fearing people would laught at him because of such a huge age difference. No wonder why he kept a low profile event.
Congrats Shoaib, I'm wondering, whats the point of emphazing on bride's age? She's 20, Adult and can marry both legally & as per religious point of view..
Jodi salamat rahey......I wonder if shoib was still unmarried......
20 year old!!! How old is this dude ?
Who is the lucky girl?
Could he not find anyone his age??? But anyway congratulations to the newly Wed :)
Congratulations shoaib!!!
But my question is to everyone and not just to a particular country or religion. We have seen marriages with this age gap around the world and everywhere. Is it good for happy family life, is it not almost a generation gap and expectations would be totally different?
Just curious!!!
Dont you think that 20 year old is too old for him??
There is no reason to doubt, but media is reporting that bride is much younger than 20.
Congrats Shoaib.
While its pleasing to know about a good event, but who is this guy in the video without introducing himself and why does he have to take pains in explaining what happened, khahmkhah making the event a suspect. The details he has explained are already those which are always narrated by Nikah Khwan while performing the ceremony. The only positive thing in his statement that I liked was that there were no 'rasoomat'. Best of luck to Shoaib, the bride and their families for a very happy, successful and prosperous life.
Congratulations to the couple
Aha!! Finally clean bowled over. Heartiest Congratulations on the new phase of his life.
Good news Shoaib. May Allah bless you both.
Who cares?
The age gap disturbs me. Am I alone?
" the nikkah ceremony the 'haq mehr' was set at Rs500,000 adding that the newly-wed couple had departed from Haripur after the 'rukhsati' event "
Could this report get more tacky ?
As long as the marriage is between two consenting adults (at least of 18 or 19 years of age) who are not of direct close family patronage, it should be no one's business.
Congratulations to sohaib...one of my favourite bowlers of pakistan
Congratulations!!! How great of you to conduct the ceremony in simple way...Our society need to learn this.
Finally our Railways Express had derailed(married),I wish happy marriage to a man of miraculous bolwing at one point in time
Congrats to the Rawalpindi Express. God bless him and his new family.
20 Years old?
Congrats to the Rawalpindi Express. May Allah bless him and his new family with joy and happiness...
Hope it is a marriage 'death do us apart'. There is always apprehension when one 1/2 has gained celebrity status. Good luck to them.
@Iqbal bhai You are definitely not alone and its none of your concern.
@Iqbal bhai If the family of Shoaib and his new wife is not disturb, why are you getting disturb for no reason. Leave them alone.
we must appreciate the fact that he got married in a simple ceremony rather than being concerned about the age of the bride! get your priorities right Dawn! Pathetic way to report the news!
Both of them are old enough to make their own decisions. People should stop commenting on the age. It's not like he married a minor. Congrats to the bride and groom!
Many congratulations to the couple.
I think when Shoaib clean bowled Sachin and Dravid in consecutive balls this girl was a just born.
Hey Guys.. why the fuss about the age difference???? Both are adult and both are marrying by consent.
Did anyone make a fuss when Saif Ali Khan Pataudi married Kareena.. There is a age difference even over there. When Aamir married Kiran?? Its not a forcible marriage Why targeting this lad?? Respect this event. Its a big day for him.
What's wrong with you guys? He can marry whoever he wants. Both are adults and there's no such thing as age difference.
Congrats to our Hero. You've served the country well. Hope you start the next chapter of your life well with Allah's Blessings.
Congratulations to Shoaib and his bride, and best wishes for a long and happy marriage! For those who have commented on the age difference, let us not forget the other celebrity weddings where the groom was much older than the bride. Dilip Kumar was 44 when he married a 22 year old Saira Banu. And IIRC, Rajesh Khanna was 34 when he married Dimple who was 17. Who are we to have any say in an essentially Mian-Bibi affair?
Congratulations. May God bless you with a son.
Congratulations shoaib bai. Heartiest congratulations to the Rawalpindi Express for getting married and embarking upon a new journey in life. Best of luck to him and his family. Wishing Shoaib Akhtar a happy, prosperous and successful married life.
I'm a little surprised that no one finds this a little alarming. In a world where there is an increasing encouragement for women to wed once they acheive both physical as well mental maturity, people are celebrating and congratulating the marriage of a 20 year old to some one almost twice her age.
Dont go after the age. When Dilip Kumar married Saira Bano the age differene was almost 20 years. Still they made an happy journey . So let us wish our express bowler all the success in life and PCB must use his services to groom more Rawalpindi expresses.
@Randomstranger - why only son ?? typical sexist comment and chauvinistic mentality.
@nadeem What a wasted talent!!
He wasn't looking for a 39 year old.
10 year age gap is regarded as a 'generation' gap.
What is wrong having a daughter?
I totally agree with you Sam. I have 2 wonderful daughters and never ever thought about having a son.
@Viking Star why weird? she is adult and it is his personal decision to marry anyone who is adult and mature enough to live a married life with him. Best wishes for both of them.
@amer I have read the big book. It could have been worse.
May Allah bless the bride and groom, there is nothing wrong with anybody age. perfectly legal marriage and very nice to see it happening like in a Muslim way. Best of luck dude.
@Dr Ali Raza on a TV show in India Shoaib said his bride was 17 years and there were protests.
She has married the name not the man..
There is this news and there was another news that the parents of a 17 year old girl slit her throat and that of her 31 year old husband because they fell in love and got married.
If the law of 'honor' in this wretched land called Pakistan, is to be upheld, then accroding to the illiterate Pakistani Jurga, Shoaib should run - and run fast!!
Many congratz to Shoaib!
I dont understand why age becomes an issue to criticize or gossip about in our society. We are muslims n yet so close minded. Islam doesnt lay any such conditions n we dont even learn from Prophet Muhammad(PBUH) life either.
Is he mentally stable to get married?
@Dr. Salaria, Aamir Ahmad if you call him Rawalpindi express. he will always be late never in time... Yesterday's the past, tomorrow's the future, but today is a gift. That's why it's called the present. so consider the present as your gift and enjoy your marriage life but don't be late....
@Randomstranger That is so sexist. Why is a daughter seen as a burden? Regardless of gender a child is a blessing.


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WEST LINN, Ore.--(BUSINESS WIRE)--(Haleo Corporation), the leader in business valuation software, business market value data analysis and reporting, has announced the release of its latest business valuation product: ValuAdder® 5 business valuation software.
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Nursing is one of the noblest professions in the world, which reminds us of Florence Nightingale. In the healthcare sector, nurses play a leading role from the operating theatre to the general ward and form an integral part. The largest group of workers in the health sector is those in the nursing occupations as nurses and nursing assistants. With the expansion of the health industry, the demand for nurses is also increasing making the career very attractive. As knowledge based discipline devoted to the betterment of humanity, nursing has developed into an art as well.
Recognized as an altruistic behavior, this profession includes a wide range of responsibilities and candidates need to dedicate time, personal effort and most of all, determination in caring for people's well-being. As one of the most diverse of all healthcare professionals, it is all about blending together the science of today's health care practices with the art of caring.
Generally nurses are required for the bedside care of patients, but it changes with the qualification and the working environment. They starts with bedside care, gradually acquires specialized skills which helps them to manage special group of people like intensive care patients, psychiatric, pediatric, etc. They provide medical care for patients, take care of those needing surgery, accident and focuses on the patient's medical and emotional needs. Besides they need to provide medication, keep records of the patients' progress, help in setting up and operating medical equipment, administration and several other schedule tasks.
Scope in nursing career
There is a great scope in nursing career- it is at a time very interesting, rewarding and challenging. The quality of health care services is improving and raising the demands for skilled and specialized nurses. One can get employment in specialized fields like Surgical, Medical, ICU, Telemetry...


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|» Products
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Many film critics (and other viewers) compare the current 3D-movies to the beginning of film history, when the moving pictures were just another sensation for carnival magicians. Martin Scorsese puts this comparison in his award-winning "Hugo" together, when he tells a story from the early times of cinema by using the new (and in this case well done) 3D-technique of our days – in this way, the 3D-effect wins an impact and feels like more than just a simple eye-catcher. Besides that, "Hugo" is a well narrated homage to the art of movies, which entertains as an adventure full of charming details and experienced references to film history – well done, Marty, nice job!
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Thai-New Zealand Formal Negotiations Start
Media Statement
14 June 2004
Thai-New Zealand Formal Negotiations Start
Negotiations on a Closer Economic Partnership trade agreement with Thailand start in Wellington tomorrow, Trade Negotiations Minister Jim Sutton said today.
Mr Sutton said an information bulletin outlining the process for the negotiations and New Zealand's objectives was available on the Foreign Affairs and Trade Ministry website www.mfat.govt.nz. It was also being circulated to people who had registered an interest in the negotiations.
He encouraged businesses and others with an interest in Thailand to make their interests known to the ministry's negotiators.
"It is good to see formal talks getting underway. There is a lot to gain on both sides to successfully conclude this process.
"Since the agreement last October between Prime Minister Helen Clark and Thai prime minister Thaksin to conclude a CEP, our two countries have worked closely together on a joint study and on preparing the ground for negotiations.
"It's clear from discussions with the Thai Commerce Minister at last week's APEC trade ministers' meeting in Chile that there is a strong shared commitment to the process and to the November target for finishing negotiations.
"The joint study showed there would be mutual benefits to New Zealand and Thailand from building closer trade and economic ties through such a trade agreement."


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Thursday, March 31, 2016
The young women glance around
comparing themselves to each other,
ranking their competition,
with a much practiced eye for details.
Constantly anxious of their appearance
they worry through their days,
vying for the attention
of the young men.
The days have become years,
and many years have passed.
In the security of an eternal relationship,
her anxiousness has mellowed
into a comfortableness with herself.
Though sometimes,
glancing at her matronly figure,
she wistfully remembers
those days of competition.
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Cumberland Heights, Nashville Addiction Treatment
8283 River Road Pike, Nashville Tennessee, 37209
- Contact: Intake Department
- www.cumberlandheights.org
- (800) 646-9998
About Treatment Center
Located in Nashville, Tennessee, Cumberland Heights, Nashville Addiction Treatment offers treatment for Addiction, Alcohol Abuse and Drug Abuse. The addiction specialists at our multi-licensed treatment facility administers a comprehensive assessment to the patient in order to determine the best course of action for their treatment plan. We offer Intensive outpatient treatment, Long-term residential and Short-term residential that are customized to suit the patients' needs. In these treatment plans, we teach addicts how to develop coping skills so that they can learn to deal with any situation that comes their way without resorting to drugs or alcohol as a means for a temporary escape. It is within these coping skills that will ensure the success of an addict's recovery. Our treatment approaches include Expressive Art Therapy, Family Based Treatment and Intervention. At Cumberland Heights, Nashville Addiction Treatment, we believe that anyone can recover, despite how deep they are in their addiction. We will do everything in our power to help our patients heal from the wounds inflicted by addiction. We treat Adolescents, Adults and Seniors or older adults and accept Acclaim, Aetna and American Behavioral, plus many other insurances. We are here to help break the cycle of addiction so that our patients can get their lives back on track. For the convenience of the patient, we have Child / Family Boarding, Free Parking and TV in Waiting Room that will make their stay here more comfortable and enjoyable.
- License No.: L000000016105
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- Certificate: The Joint Commission/ID# 3395
- Certificate Date: 2015
Rehab Center Details
- 12 Step Program
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American Express, Cash or self-payment, Check, Health Savings Account, Mastercard, Visa, Wire.
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Recommended Treatment Centers
White Sands Tampa Center2011 N Wheeler Street
Plant City, FL 33563 Learn More 844-388-1979
Mountain Peak Recovery14048 South Highway 89
Spanish Fork, UT 84660 Learn More 844-376-9438
Mount Sinai Wellness Center330 Mt Sinai Drive
Dahlonega, GA 30533 Learn More 844-497-7482
JourneyPure Bowling Green2349 Russellville Road
Bowling Green, KY 42101 Learn More 844-516-8374
White Sands Treatment Center1820 Colonilal Blvd
Fort Myers, FL 33907 Learn More 844-377-5328
Palm Partners Recovery Center816 Palm Trail
Delray Beach, FL 33483 Learn More 844-291-7058
Windward Way Recovery2801 Bristol St.
Costa Mesa, CA 92626 Learn More 844-469-5798
Destination Hope6555 Powerline Road
Fort Lauderdale, FL 33309 Learn More 844-528-4571
Google Reviews
Twice they've saved my life, once in the mid-90's, and once again in 2002. I'm a healthy, happy, successful person today because they cared enough to work with me in my darkest, most desperate times. Thank you!
No Fortune Cookie, 4 months ago
My company worked with Cumberland Heights on their new website design, so my experience was different than many of the others who have visited their campus. I was very impressed with the dedication of the staff and the campus was nicer than many state universities. I would recommend Cumberland Heights to anyone in the Middle TN area who is in need of their services.
Simon Carr, 4 months ago
This place is truly sacred,it saved my life. Staff is wonderful and the food is great. I also got to meet some wonderful friends that I'm sure will be life long. If your not serious about recovery please don't waste the staffs time or the bed space needed for someone who is serious. Thanks Cumberland Heights for everything.
William Seifert, 8 months ago
They have a really beautiful location far better than the all white medical feeling centers. They were so compassionate and sympathetic
Elias Turner, 9 months ago
Wonderful staff, wonderful people, great food, and beautiful scenery. Great after care programs. Note: this is a treatment center that focuses on AA/NA/CA/etc.. Only those serious about sobriety should check in... Leave a spot for those who want it.
Tyler Durham, 1 year ago
Cumberland Heights introduced me to the program and fellowship that saved my life. I was a hopeless drug addict waiting to die and Cumberland Heights showed that another way of life was possible. The alumni connections were invaluable. On the weekends, alumni in recovery return to campus to share their experience, strength, and hope and bring back a taste of life and recovery in the real world, outside of treatment. I was able to recognize people that had been where I was and knew exactly the pain and misery of active addiction. It inspired me to see how they had changed their lives and to see them laughing and having fun. It showed me that recovery was possible and that this way of life was also a life a joy. I'm incredibly grateful for Cumberland Heights and I will never be able to repay what they have given me.
Nicholas Salazar, 1 year ago
Cumberland Heights gave me the tools i needed to stay sober and continued to support me and love me as i transitioned into the world. The staff loved me unconditionally and i was able to interact with alumni which showed me that life outside of treatment was possible. I also met some of my best friends through this magical place and continue to be grateful for them.
Olga D'Angelo, 1 year ago


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chub_tor 15:59 21 Oct 2008
You have the blame pointed in the right direction. Usually the easiest to check is the memory, if you have two sticks take one out at a time and reboot to see if one of them is flaky. New DDR2 memory is very cheap now.
Power supplies can only be checked by substitution unless you have a friendly local repairer who might have a test rig. Again not too expensive to buy and always worth an upgrade.
If it runs OK once booted up it's not likely to be the hard drive or the motherboard or the graphics card come to that.


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Awww thank you so much! <33 Your sweet comment really means so much to me!
I think I've started on the Nouk curly hair, I'm not entirely sure though! I will check tonight (first gotta finish some other project n___n) but if I started on it and I havn't finished yet, it's probably because the mapping is wonky :/
Anyway, Ill give the squid hair a shot too! Actually, most of Nouk's hairs are on my list :D she's awesome! 8)
Summerdream Sims - Post a comment
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Neena (summerdream) wrote on November 2nd, 2009 at 05:11 pm
Re: Just dropping by


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Saving and Studying Tasmania's Giant Trees …Outside North America, Mifsud has found more of the world's biggest trees than anyone else (well over 120). In Tasmania, he's located more than 60 giants. The day I spent with him in a valley south of Hobart, he found four Eucalyptus regnans that qualify for protection, including one with a girth of 21.6 meters (almost 71 feet)—big enough for 20 people to stand comfortably inside its hollow base.
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For details contact restaurant


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Rodeway Inn - Pocatello, ID
Rodeway Inn - Pocatello, ID
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835 South 5th Ave.
Pocatello, ID 83201
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Quirky Tourist Attractions in or near Idaho
While in Idaho, you might enjoy a visit to:
Pocatello, Idaho: Don Aslett Museum of Clean
Over 6,000 vacuums, dust busters, and rug beaters, collected by spic-and-span businessman Don Aslett, are part of his mission to spread the virtues of cleanliness.
Soda Springs, Idaho: Largest Man-Tamed Geyser
First erupted in 1937, when a local man drilling for heated water for a swimming pool punctured an underground chamber. Now on a timer so it erupts every hour, on the hour -- unless it's windy.
Arco, Idaho: World's First Nuclear Power Plant - Tour
Desolate region is proud of building over 50 nuclear reactors, including the first nuclear power plant, which is open in summer for visitors.
Rigby, Idaho: Birthplace of Television
Rigby was the boyhood home of Philo Farnsworth, who invented TV. See his eyeglasses, his hat, and his violin in the local historical museum, along with a flag that went to the moon.
© RoadsideAmerica.com, Doug Kirby, Ken Smith, Mike Wilkins. Information provided as an unofficial resource on attractions as a convenience to our visitors.
Terms | Privacy | Pocatello Car Rentals
Pocatello Hotel ChainsBest Western
Comfort Inn
Fairfield Inn
Hampton Inn
Holiday Inn Express
La Quinta
Red Lion
Rodeway Inn
Super 8
TownePlace Suites
Cities Near PocatelloBlackfoot
Fort Hall
Lava Hot Springs
Airport - TransportationPocatello Regional Airport (PIH)
Art Galleries & DealersPocatello Art Center
Hospitals & Medical Care FacilitiesPortneuf Medical Center
Bannock Regional Medical Center
Pocatello Regional Medical Center
MuseumsBannock County Historical Msm
Idaho Museum-Natural History
Universities & CollegesIdaho State University
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The sixth highest-ranked university in the world has reportedly sponsored a guide to "fight transphobia" and "get the facts about gender diversity."
According to Campus Reform, Harvard's BGLTQ Student Life released the guide, which was given to students on campus, in a hope to 'educate' them on gender diversity.
"Sex assigned at birth and gender identity are not necessarily the same," states the flyer, going on to say that a person's anatomy is not aligned with "gender identity, gender expression, sexual orientation, hormonal makeup, physical anatomy, and/or how one is perceived in daily life."
The guide also declares that "gender is fluid and changing," and "there are more than two sexes". It also claims that someone's gender can "can be affirmed and/or expressed in many ways," and can "change from day to day."
The flyer also claims "transphobic misinformation is a form of systemic violence," and that "fixed binaries and biological essentialism, manifest in gendered language, misgendering someone, and the policing of trans bodies, threaten the lives of trans people."
See the flyer – shared by Campus Reform – below.
Statistics that are used in the guide come from a group called "Trans Student Educational Resources" that claim such things as "trans people of color are 6x more likely to experience physical violence when interacting with the police than with white cisgender survivors of violence," and "80% of trans students feel unsafe at school because of their gender expression."
Several students expressed outrage but declined to comment on the record about the school's use of funds, as they feared repercussions.
Twitter users, however, have no such fears.
@campusreform No wonder millennials are so screwed up.
— PP In TX (@DoublePTX2) April 20, 2017
@campusreform Institution of higher learning my ass
— John Collins (@VStrom_John) April 20, 2017
@campusreform i hate when that happens i never know if im supposed to pee standing up sitting down
— nana (@Flapjack63Sw) April 20, 2017
@campusreform Is this a clever Monty Python 'real-world' prank or is it really happening ?
— Freight Dog (@Jon__Clark) April 20, 2017