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Give us a call on +353 (0)41 9830142 or
News & Events
Boom … Budget 2018 Analysed!
Minister Paschal Donohoe announced Budget 2018 at lunchtime. By tea time the team at www.McInerneySaunders.ie were ready to deliver their analysis to clients and guests. Every year this dynamic, partner-led firm seeks to provide clients with expert analysis guided by the insights and experiences of entrepreneurs and business leaders. McInerney Saunders burst through the boring … Continued
Ireland's Best Young Entrepreneur 2018
We were delighted to have won the tender to manage IBYE 2018. This is our 4th year working on this exciting project. IBYE is a national competition for young entrepreneurs aged between 18-35. There are three categories: Best Idea, Best…
IBYE 2018 – Could you be Ireland's Best Young Entrepreneur 2018?
We are delighted to have been selected by the Department for Business, Enterprise & Innovation, the Local Enterprise Office Network and Enterprise Ireland to project manage IBYE 2018. If you are aged between 18 – 35, have a business idea…
Focus on Sales, Profit and Cashflow
The team at WhiteLight Consulting are delighted to manage the Fingal Enterprising Women Network hosted by Local Enterprise Office Fingal. This very successful network has a membership of over 600 and meets quarterly to network and learn. Our next event…
Fabulous Flavours of Fingal!
This year's Flavours of Fingal played host to a huge selection of artisan food vendors, trade stands, a vast number of entertaining activities for young and old alike, as well as over 80,000 visitors to the site over two days!…
Everyone Head For Flavours of Fingal
Coming up this weekend on Saturday 24th and Sunday 25th, we have the fabulous Flavours of Fingal, which takes place in Newbridge House, Donabate. As Ireland's largest premier county show, this year's event is set to be the finest, free family fun festival of the summer. We're so excited to head up corporate hospitality on … Continued
An Intern's Eye View: The Irish Maritime Festival
Our intrepid intern Tori Wilton is back to give us her perspective on The Irish Maritime Festival 2017, which took place at Drogheda Port recently. Here's her "Intern's Eye View": Drogheda Port once again played host this summer to the Irish Maritime Festival, which was a roaring success. The Maritime Festival, if you haven't already … Continued
Maritime Festival Continues To Grow
The river Boyne certainly took centre stage last weekend as the Irish Maritime festival sailed into Drogheda Port. From the arrival of the ships at Mornington on Friday afternoon to their departure on Monday morning, the Port was filled with…


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Consolidated Auto Service Center, Inc.
ReScore Reviews™ (4)
98% Would Recommend
Latest Review about 3 years ago
Featured Business
Burlington Auto Body310 East Burlington
LaGrange, IL 60525
4.2 miles away
"Shannon said the person she worked with was polite and the service was quick."
Review for Consolidated Auto Service Center, Inc.
Charlie H. on 11/23/2013
Charlie said they were really on top of things at this business. They were ordering parts as soon as they had his vehicle at the business. They told him it would take 5 days and it only took 3 days. They kept him updated every step of the way. Mike was really good on following up and keeping him informed through the process.
Category: Collision
Service Date: 11/07/2013
Review Created: 11/23/2013
This review was collected via phone interview by Customer Research Inc.

subset: commoncrawl


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REMEMBER Mr Snowden, who made headlines in 2013 after leaking a cache of documents that exposed the NSA (National Security Agency) spying on American citizens, famously asked lawyers advising him to "hide their cellphones in the refrigerator of the home where he was staying, to block any eavesdropping."
YES THAT SNOWDEN AND AMERICAN HACKER ANDREW HUANG LIVING IN SINGAPORE Wh0 ALSO HAS A PHD IN electrical engineering from MIT. THEY BOTH BEEN SECRETLY WORKING ON A I PHONE 6 CASE.
WHATS SO SPECIAL ABOUT THIS CASE YOU ASK? It doesn't block cell phone signals, but it does make sure the device stops transmitting data when you put it on "airplane mode."
Most people think that "airplane mode" pulls a smartphone off the grid. It doesn't. On the latest iPhones, for example, the device still transmits GPS signals when it's in "airplane mode."
Governments can take advantage of that by hacking phones. (That's how the NSA can "turn on" your phone remotely.)
And governments do it regularly. It's suspected that's how the Syrian government tracked, targeted and killed American journalist Marie Colvin with a mortar barrage.
In a research paper, Snowden describes a sleeve that slips over the bottom of an iPhone, connects to the SIM card port, and watches for outbound signals. An alarm would chime if it detects an unwanted signal, plus it provides extra battery juice. They say that they are also considering to add a "kill switch" option that would forcibly disconnect power to the phone in the case that a radio is found to be errantly transmitting
"Front-line journalists are high-value targets, and their enemies will spare no expense to silence them," Snowden wrote. "Unfortunately, journalists can be betrayed by their own tools. Their smartphones, an essential tool for communicating with sources and the outside world — as well as for taking photos and authoring articles — are also the perfect tracking device."
In their paper, they describe how they explored the Hua Qiang electronics markets in the Chinese city of Shenzhen. Surrounded by iPhone repair stations, they found lots of spare parts and repair manuals — which included detailed blueprints of the iPhone 6.
The pair presented their findings at the Massachusetts Institute of Technology's Media Lab on Thursday morning.
They expect to develop a prototype of this case in the next year. But don't get your hopes up. This is a tiny, experimental effort.
"The project is run largely through volunteer efforts on a shoestring budget," the duo wrote.
The device is currently just an academic project and still far from the mass market. The pair is hoping to develop a prototype within the next year and say they may then seek to create cases for other smart phones as well.


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POLL: What's Your Favorite Tim McGraw Song?
A readers' poll to determine the best Tim McGraw song.
A readers' poll to determine the best Tim McGraw song.
No one makes old sound quite as cool as Tim McGraw on songs like "How I'll Always Be."
I would have gone nuts if I was in this room.
Finally, something and someone we want to vote for. Am I right?
Tim McGraw turned to Facebook this Mother's Day to show appreciation for both his own mother and the mother of his own children, his wife Faith Hill.
A photo gallery of country stars with their mothers.
A list of the top wedding songs in country music.
A list of the Top 10 songs from country music superstar Tim McGraw.
A photo gallery of grin-inducing pictures of country music stars.
Tim McGraw recently announced his partnership with Narus Health, a Nashville-based company that is focused on improving the quality of life for people with terminal illnesses.
Faith Hill couldn't be happier for her husband Tim McGraw after his performance at the 2016 ACM Awards Sunday night (April 3).
The big winner at the 2016 ACM Awards was Chris Stapleton, but none of his big wins make our Top 5 from the April 3 ceremony. The show was performance-heavy, and there are some spectacular performances to talk about today and all week.


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Friends can say the most hurtful of things and years down the line, that stuff can still affect you. Worse still, they will not know the damage they caused. I can only ask for forgiveness from those I have hurt because it deeply sucks.
On that note I shall forgive and forget. Or speak myself to that place.


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Conceived and designed the experiments: QD ZC HW. Performed the experiments: QD PM TW WQ WQ. Analyzed the data: QD PM TW WQ WQ FW JY AGY. Contributed reagents/materials/analysis tools: WQ WQ FW ZC AGY. Wrote the paper: QD FW JY.
Previous work has shown reduced expression levels of let-7 in lung tumors. But little is known about the expression or mechanisms of let-7a in prostate cancer. In this study, we used in vitro and in vivo approaches to investigate whether E2F2 and CCND2 are direct targets of let-7a, and if let-7a acts as a tumor suppressor in prostate cancer by down-regulating E2F2 and CCND2.
Findings Real-time RT-PCR demonstrated that decreased levels of let-7a are present in resected prostate cancer samples and prostate cancer cell lines. Cellular proliferation was inhibited in PC3 cells and LNCaP cells after transfection with let-7a. Cell cycle analysis showed that let-7a induced cell cycle arrest at the G1/S phase. A dual-luciferase reporter assay demonstrated that the 3′UTR of E2F2 and CCND2 were directly bound to let-7a and western blotting analysis further indicated that let-7a down-regulated the expression of E2F2 and CCND2. Our xenograft models of prostate cancer confirmed the capability of let-7a to inhibit prostate tumor development in vivo.
These findings help to unravel the anti-proliferative mechanisms of let-7a in prostate cancer. Let-7a may also be novel therapeutic candidate for prostate cancer given its ability to induce cell-cycle arrest and inhibit cell growth, especially in hormone-refractory prostate cancer.
MicroRNAs (miRNAs) are endogenous, noncoding small RNAs 20–25 nucleotides in length [1], which play an important regulatory role through complimentary binding of the 3′ untranslated regions (UTRs) of target genes. Binding results in the degradation of the target mRNA and inhibition of translation [2]. Many miRNAs are associated with cancer, and are involved in cell development, differentiation, proliferation, and cell death [3]. Many reports have indicated that miRNAs can be useful for cancer diagnosis and therapy [4]. let-7 was first identified in Caenorhabditis elegans [5]. It is nearly undetectable in the embryonic stage of development, but becomes more abundant in later stages of development [6]. Previous work has shown reduced expression levels of let-7 in lung tumors compared to normal lung tissue. let-7 slows cellular proliferation by down-regulating the oncogenes RAS/c-MYC and HMGA-2 at the translational level [7], [8]. The same tumor suppressive functions have also been reported for let-7 in colon cancer [9].
In silico analyses of potential let-7a targets (www.targetscan.org andwww.microrna.org) reveal that both E2F2 and CCND2 are possible targets of let-7a. E2F2 and CCND2 are cell-cycle regulators and aberrant expression of them can lead to abnormal cellular proliferation. Our preliminary experiments indicate that protein levels of both E2F2 and CCND2 are up-regulated in the PC3 prostate cancer cell line. Little is known about the expression or mechanisms of let-7a in prostate cancer. In this study, we used in vitro and in vivo approaches to investigate whether E2F2 and CCND2 are direct targets of let-7a, and if let-7a acts as a tumor suppressor in prostate cancer by down-regulating E2F2 and CCND2.
All samples were obtained from patients who signed informed consent approving the use of their tissues for research purposes after operation. The clinic pathological factors of the 26 patient were showed in Table S1. The use of human tissues in this study was approved by the Institutional Review Board of the Fourth Military Medical University and was in accordance with their guidelines(No 2008039085). All experiments involving animals were conducted according to the Animal Welfare Act and approved by Animal Care and Use Committee of the Fourth Military Medical University. (Approval number 200804052353).
Human prostate cancer cell lines LNCap, DU145, PC3, and PrEC (prostate epithelial cells) and human embryonic kidney cells HEK293A were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were cultured in RPMI-1640 (Gibco) supplemented with 10% fetal-calf-serum and penicillin (100 U/ml). Cultures were maintained under an atmosphere containing 5% CO2 (Forma Scientific). Twenty-six freshly resected prostate cancer specimens and their adjacent non-tumorous specimens were collected from the Department of Urology in Xi'jing Hospital. The specimens were immediately frozen in liquid nitrogen and maintained there until use.
Let-7a was amplified and purified by miRNA isolation kit (Invitrogen, Carlsbad, CA) according to manufacturer's protocol. PCR primers for let-7a were: 5′-GAATTCTCACACAGGAAACCAGGA-3′ (forward) and 5′-CTGCAGTCAGGCATTTAAGTGACCG-3′ (reverse). Let-7a PCR products were cloned into the EcoRI/PstI cloning site of a pcDNA3 plasmid containing green fluorescent protein (GFP) to form the plasmid pcDNA3-let-7a-GFP. Cell transfection was performed with lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol. Briefly, PC3 cells were plated in six well plates and grown without antibiotics to 70–90% confluence. Each well received 6 µl of lipofectamine reagent containing 3 µg of plasmid DNA. Three wells received pcDNA3-let-7a-GFP plasmid while the other wells received the empty vector, pcDNA3-GFP. G418 (400 µg/ml) was added to cells 24 h after transfection. Mixed clones were screened and cultured for an additional 4 weeks. PC3 cells transfected with let-7a were designated as PC3-let-7a-GFP, the negative control (cells transfected with the empty vector) was designated as PC3-GFP. PC3 cells were also transfected with 30 nM of synthetic let-7a (mimics), and synthetic negative control miRNAs (NC). Lastly, a synthetic let-7a-inhibitor sequence and synthetic let-7a-inhibitor negative control (NC inhibitor). Sequences of synthetic hsa-let-7a, negative control, hsa-let-7a inhibitor and inhibitor negative control were showed in Text S1.
For let-7a, Total RNA was extracted from samples using a miRNeasy Mini Kit (Qiagen). cDNA was synthesized using TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems). Real-time RT-PCR was performed with TaqMan MicroRNA Assay kit (Applied Biosystems). The primer for let-7a was 5′-GAGGTAGTAGGTTGTATA-3′. For E2F2 and CCND2, total RNA extraction and real-time RT-PCR were performed using SYBR® GreenER™ Two-step kit (Invitrogen, Carlsbad, CA). PCR primers for E2F2 were: 5′-GAGCTCACTCAGACCCCAAG-3′ (forward) and 5′-AACAGGCTGAAGCCAAAAGA-3′ (reverse). PCR primers for CCND2 were: 5′-AAGAATTCCTCCTCAATAGCCTGCAGCAGTA-3′ (forward) and 5′-GCGGGATATCGACCTGTGAGAATTCGAT-3′ (reverse). All protocols were carried out according to manufacturer protocols. The expression level of let7a was normalized to RNU6B. The expression level of E2F2 and CCND2 were normalized to GAPDH. Real-time RT-PCR was performed by using 7500 Real-time RT-PCR System (Applied Biosystems). PCR was performed under the following conditions: 50°C for 2 min, 95°C for 10 min, followed by 50 cycles at 95°C for 15 s, and 60°C for 1 min. Each sample was run in triplicate.
PC3 cells and LNCaP cells transfected with either NC or let-7a (mimics), or NC inhibitor and let-7a inhibitor were plated on 96-well plates at 1×104 cells/well. Viable cells were measured 1, 2, 3, 4, and 5 days after plating. After incubation with 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT), the cells were lysed in 150 µl of 100% dimethylsulfoxide (DMSO) and UV-visible absorbance was read at 490 nm using the 96-well plate reader. Each sample was run in triplicate.
Briefly, 2 ml of 0.5% agar was added to each well of a 12-well plate. Detached PC3-let-7a-GFP and PC3-GFP cells were mixed with the topagarose suspension (final concentration 0.3%), and then the cells were layered onto the 0.5% agarose underlay. The number of foci >100 µm were counted after 18 days. Each experiment was performed in triplicate.
PC3 cells and LNCaP cells transfected with either NC or let-7a (mimics), or NC inhibitor or let-7a inhibitor were harvested 72 h after transfection, washed with cold phosphate buffered saline (PBS), and fixed in 1ml of 70% ethanol. After overnight incubation at 4°C in ethanol, cells were washed in PBS and suspended in 500 µl propidine iodide (PI) 30 min before flow cytometry. Populations in G0-G1, S, and G2-M phase were measured by flow cytometry (EPICS XL, Coulter, Miami FL) and the data were analyzed by using Multicycle-DNA Cell Cycle Analyzed Software. The measurement was performed in triplicate.
To investigate whether E2F2 and CCND2 expression were regulated by let-7a, a dual-luciferase reporter assay was performed. The 3′-UTR of CCND2 and E2F2 were amplified by PCR respectively. Amplification of CCND2 used the following primers: 5′-GAATTCATGAGTTCTTCGTACTGG-3′ (forward) and 5′-GATATCCCATCATGAT GAGGATAC-3′ (reverse). PCR products were 398 bp and contained two binding sites for let-7a. The mutated 3′-UTR of CCND2 was synthesized after point mutations were made in several bases within the binding sites of these PCR products. Amplification of the 3′-UTR of E2F2 used the following primer sequences: 5′-GAATTCCTCTTCGACTCCTACGAC-3′ (forward) and 5′-GATATCTGTGCTTCTT GGTACGTCG-3′ (reverse). The PCR products were 415 bp and contained two binding sites for let-7a. Mutated 3′-UTR of E2F2 were synthesized after several point mutations were made in the binding sites PCR products. After restriction digestion, amplified genes were cloned into the corresponding sites of a reconstructed pGL3 expression vector. The pRL-TK control vector expressing Renilla luciferase (Promega) was used for normalization of cell number and transfection efficiency. Cotransfections of synthetic let-7a sequence (mimics) and NC, or let-7a inhibitor and NC inhibitor were also performed in human embryonic kidney cells HEK293A by using lipofectamine 2000 (Invitrogen). Firefly and Renilla luciferase activity was determined by Pikkagene Dual Luciferase Assay System (Toyo-B-Net).
The following antibodies were used for western blotting: mouse anti-E2F2 (1200 dilution, Santa Cruz Biotech); mouse anti-cdk4 and mouse anti-cyclin D2 (1300 dilution; BD Biosciences, San Jose, CA); mouse anti-k-RAS (1300 dilution; Cell Signaling Technology, Beverly, MA); rabbit anti-β-actin (14000 dilution, Sigma). Twenty-five micrograms of prostate cancer specimens and their adjacent non-tumorous specimens' protein as well as PC3-let-7a-GFP and PC3-GFP protein were electrophoresed in 10% SDS–PAGE minigels and transferred onto Hybond C nitrocellulose membranes (Amersham Life Science, Buckinghamshire, UK). After incubating with primary antibodies at 4°C overnight, the nitrocellulose membranes were washed three times with Tris-Buffered Saline Tween-20 (TBST) and then incubated with fluorecein isothiocyante (FITC)-conjugated, goat anti-mouse or goat anti-rabbit IgG (1500 dilution, Sigma) for 1 h at room temperature. After washing with TBST, blots were visualized by using the Odyssey Infrared Imaging system (LI-COR Bioscience, Lincoln, Nebraska USA). Each assay was repeated three times.
Five nude mice were subcutaneously injected with ~1×106 PC3-let-7a-GFP or PC3-GFP cells at a single site of the back. The weight of the tumor and the weight of the mouse were measured at the time of sacrifice (4 weeks after injection). Western blotting was performed to investigate whether E2F2 and CCND2 were down-regulated in nude-mouse xenograft model. Tumor suspensions were treated with lysis buffer and western blotting was done according to the procedures described above.
Differences between each group were determined by the T test or Mann-Whitney U test using Statistical SPSS software package (SPSS Inc, Chicago) (p<0.05 was regarded as significant).
Real-time RT-PCR revealed that the expression levels of let-7a were decreased by ~43% in resected human prostate cancer samples compared to the adjacent non-cancerous samples. The prostate cancer cell lines LNCap expressed only ~70% as let-7a than normal prostate epithelial cells PrEC. Additionally, the amount of let-7a expression in PC3 cells and DU-145 were about 50% of that observed in PrEC. These results indicate that let-7a is decreased in prostate cancer, including the androgen-independent cancers PC3 (Fig. 1, * p<0.05; ** p<0.01).
After transfection, the expression level of let-7a in PC3-let-7a-GFP was ~300% higher than in PC3-GFP by real-time RT-PCR; A ten-fold increase in expression of let-7a was observed in PC3 cells transfected with let-7a (mimics) versus those transfected with NC (Fig. 2A, p<0.01). This indicated that transfection was appropriately performed. Colony formation analysis indicated that the colonies formation ability of PC3-let-7a-GFP cells is ~40% less than that of control cells (Fig. 2B, p<0.01). MTT growth curves indicated that from the second day, the survival of let-7a transfected PC3 cells and LNCaP cells are significantly less than that of negative control, and the survival of let-7a inhibitor transfected PC3 cells and LNCaP cells are significantly more than that of NC inhibitor transfected cells (Fig. 2C, p<0.05). Flow cytometry showed that the percentage of let-7a transfected PC3 cells and LNCaP cells in the G0-G1 phase was ~30% (PC3) and 16% (LNCaP) higher than that of negative control, which paralleled with a ~50% (PC3) and 20% (LNCaP) decrease in the S phase. the percentage of let-7a inhibitor transfected PC3 cells and LNCaP cells in the G0-G1 phase was ~23% (PC3) and 19% (LNCaP) less than that of NC inhibitor transfected cells, which paralleled with a ~33% (PC3) and 25% (LNCaP) increase in the S phase (Fig. 2D, * p<0.05; ** p<0.01). These data indicate that let-7a inhibits PC3 proliferation by inducing cell-cycle arrest at G1/S phase.
Figure 3A shows the sequences of the 3′ UTRs of E2F2 and CCND2 that represent the binding sites for let-7a. The corresponding sequences of the mutated 3′ UTRs of E2F2 and CCND2 are shown as well. No reduction of luciferase activity was observed in HEK293A cells transfected with let-7a (mimics) and mutated CCND2 or E2F2. But greater than 30% reduction of luciferase activity was observed with wild-type CCND2 and ~50% reduction of luciferase activity was observed with wild-type E2F2 (Fig. 3B, **p<0.01). Compared to NC inhibitor, there is no significant difference in luciferase activity when HEK293A cells were transfected with the let-7a inhibitor and wild-type CCND2 or E2F2 (Fig. 3C). These data support that E2F2 and CCND2 are direct targets of let-7a and endogenous let-7a in HEK293A has no interference to our experience.
Real-time RT-PCR shows that mRNA relative expression of E2F2 and CCND2 in prostate cancer tissues and in LNCaP, PC3, and DU-145 cells are up-regulated compared with their adjacent non-cancerous specimens and PrEC cells (Fig. 4A, B, * p<0.05; ** p<0.01). But compared to PrEC, mRNA expression of E2F2 and CCND2 are down-regulated after transfected with let-7a in PC3 and LNCaP cells (Fig. 4C, D, * p<0.05; ** p<0.01). Western blotting results showed no change of CDK4 protein expression between prostate cancer tissues and their adjacent non-tumorous tissues or between PC3-let-7a-GFP cells and PC3-GFP cells, the expression levels of E2F2, CCND2 increased in prostate cancer tissues compared with their adjacent non-tumorous tissues, but the expression levels of E2F2, CCND2, and k-ras decreased dramatically in PC3-let-7a-GFP cells compared with that in PC3-GFP cells (Fig. 4E). k-ras, a previously-defined molecular target of let-7a[7] was used as a positive control for these experiments. These data support that let-7a down-regulating the mRNA expression of E2F2 and CCND2 and repress protein translation of them.
Nude mice bearing PC3-let-7a-GFP or PC3-GFP xenografts were sacrificed 4 weeks after innoculation. Tumors were excised and measured (Fig. 5A). Western blotting showed that expression levels of E2F2 and CCND2 decreased dramatically in PC3-let-7a-GFP tumors compared with PC3-GFP tumors (Fig. 5B). The weight of PC3-let-7a-GFP tumors was ~80% lighter than PC3-GFP tumors (Fig. 5C, p<0.01). Furthermore, the ratio of tumor weight/body weight in mice bearing PC3-let-7a-GFP tumors was only ~6% of the ratio from mice bearing PC3-GFP tumors (Fig. 5D, p<0.01), which provides strong evidence that let-7a can inhibit tumor growth in vivo.
The inability of a cell to regulate its growth and proliferation is a distinctive feature of cancer. As critical molecules in cell-cycle regulation, E2F proteins are downstream of growth-factor signaling cascades. They influence cell growth and proliferation through their ability to regulate genes involved in cell-cycle progression [10], [11]. E2F2 is a member of the E2F family of transcription factors, and has been well-characterized as a regulator of the G1-to-S-phase transition [12]. Previous reports indicate that E2F2 has strong oncogenic capacity and can promote cell-cycle progression. Cell lines transfected with E2F2 proliferate at twice the rate of control cells [13]. For proper progression through the cell cycle, phosphorylation activity of cyclin dependent kinase (Cdk) is essential. CCNDs are expressed in the early G1 phase and bind Cdk4 and Cdk6 [14]. The activation of these kinases results in phosphorylation of other critical cell-cycle regulators such as pRb. pRb then activates E2Fs and allows S-phase entry. Accordingly, aberrant expression of CCND2 and E2F2 will lead to abnormal cellular proliferation. Overexpression of CCND2 was reported in ovarian granulose cell tumors, gastric cancer and colon cancer [15]–[17]. Similarly, upregulation of E2F2 was found in astrocytomas of different grades [18].
miRNAs post-transcriptionally regulate gene expression by binding to the 3′UTR of target mRNAs. Binding leads to the degradation of target mRNAs and reduced translation of target proteins. miRNA activity also affects the expression of genes that are downstream of direct targets and can lead to changes in global protein expression profiles. Therefore, miRNAs potentially play a critical role in the progression of human cancer. A great deal of evidence supports that aberrant expression of miRNAs occurs in diverse types of human cancer, and at different stages of cancer progression [19], [20]. let-7 has been reported to act as a tumor suppressor in some cancer types, such as lung and colon cancer[9], [21]–[23] and that reduced expression levels of let-7 is correlated with poor clinical prognosis [24].
Although this correlation between let-7 expression and disease is strongest in lung tissue, where let-7 expression is high, our study finds that let-7 causes cell cycle defects in prostate cancer cell line as well. We found that let-7a expression level is down-regulated dramatically in prostate cancer tissue and cells lines. Protein and mRNA expression levels of E2F2 and CCND2 are down-regulated in PC3 and LNCaP cells after transfection with let-7a. Our xenograft models of prostate cancer also confirm our in vitro results and show that let-7a has the ability to inhibit prostate tumor development in vivo. Our dual-luciferase reporter assay verified that E2F2 and CCND2 are direct targets of let-7a. Also, other genes associated with cell-cycle regulation have been reported to be repressed directly or indirectly by let-7. These include CDC34, which promotes the degradation of cyclin dependent kinase (CDK) inhibitor 1B and CCNA2, which promotes G1-S and G2-M phase transitions [25]. Our founding expands the family of let-7 targets, and the identifying the molecular pathways affected in cancer could further reveal the mechanisms by which let-7a inhibits cell division. Though much is still to be learned about the role of let-7a in prostate cancer tumorigenesis, let-7a provides us a new way of prostate cancer treatment via its ability to inducing cell cycle arrest and inhibiting cell growth.
Clinic pathological factors of 26 patients currently used.
Sequences of synthetic Hsa-let-7a, negative control, Hsa-let-7a inhibitor and Inhibitor negative control.
We would like to acknowledge Lijie He for excellent editorial assistance and insightful suggestions.
Competing Interests: The authors have declared that no competing interests exist.
Funding: This study was supported by grants from the National Basic Research Program of China, 2009CB521705; and the Nature Science Foundation of China, 30672097. The funders had no role in study design, data collection and analysis, decision to publish.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Contact Info / Websites
All 3 audio Reviews
The Cake Is a Lie
Rated 3.5 / 5 stars
I like it, and the game was also fun. Although, my one critique would be that, even though you named the song after Portal, there are no lyrical, nor musical references to it.
Valhalla Bound
Rated 4 / 5 stars
The Norse Gods have Spoken...
...and they are proud.
Tea and Crumpets
Rated 2 / 5 stars
It's alright. Yes, I give respect, and I've also made music with FL, so, I'm not ill-experienced. Lyrics could be a plus, but not necessary. It's a decent tune.


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So What Happens When Your Puppy Eats You Teen Daughter's Hairbrush Right Before It's Time To Leave?
I love our puppy like crazy but there isn't anything she won't eat except for her dog food but that's another story for another time. I knew something was wrong the minute I woke up this morning and saw the tiny plastic white bristles scattered … Continue Reading →


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Waterloo Wellington
This branch of CCAC services the region of Waterloo Region, Guelph and Wellington County and Southgate Township. (source)
Do they have homecare services?Yes, but home care can take many forms – from nursing care to personal support [attendant care]. In order to determine what services are right for you, the CCAC will set up an assessment with a Care Coordinator. Call your local CCAC to make a referral and we'll get you started down the right path
How do I get started?To become a patient of the CCAC, you must participate in an initial assessment to help evaluate your needs and determine whether CCAC or other services are right for you. To get started, we just need a referral. You can refer yourself, or a referral can be made by your doctor, nurse, community service provider or other health-care professional. A referral can also be made by a family member, a friend or a neighbour. A simple call to your local CCAC gets the process started.
How long is the waitlist?For 2012/2013, CCACs ensured:
50 per cent of patients referred through the community receive home care service within 5 days and 90 per cent of patients receive service within 33 days
50 per cent of patients referred through hospital get home care service within 1 day of referral and 90 per cent of patients receive service within 6 days.


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Bikram yoga championship
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During my convalescence from the flu, I read several books. It has been years since I've read that much in a short period of time. I realize how much time I spend on my phone or the Internet rather than sitting for hours with a good book. But that is a post for another time. For now, here are my thoughts on a book people have been talking about for months: Hillbilly Elegy: A Memoir of a Family and Culture in Crisis.
Prior to picking it up, the only thing I'd heard about Hillbilly Elegy was that it was the answer so many people had been searching for to make sense of the presidential election of 2016. Otherwise, I knew nothing. I was somewhat intrigued by this whole election-answer thing, but mostly I was just compelled by the title and the fact that the book is nonfiction.
I read the introduction first, obviously, and I became so excited that I actually sat up straighter in the bathtub (where I do most reading) and took notice. Vance's writing is blunt in the way that someone writes if he doesn't have too many dogs in the fight. In other words, it's a little edgy (cuss words), and he has that feeling of telling it like it is rather than massaging his story for an editorial staff wanting to make a difficult subject palatable for a wide, educated audience of NPR listeners.
Of course, that's probably exactly what he did, but he did it so well that it comes across as true, authentic and raw. Alas, I was pulled in nonetheless.
What makes Vance the perfect person to talk (and write) about what it means to grow up hillbilly (or redneck, if this was a book about Texas) is that he isn't doing it from an ivory tower of academia where so many of these stories are told from collected data and someone else's anecdotal stories. He lived it. He grew up in a poor, uneducated and dysfunctional place and time, and with a family who, bless their hearts, tried their best but often succumbed to the world around them. Vance's tale involves drug addiction, broken relationships, insecurity (of every kind), love, loyalty and the weight of expectation or, in Vance's case, a lack thereof. It's a story of how one kid struggled to keep afloat, skirting the wrong side of the law, taking care of the people who should have been taking care of him and learning to detach to survive.
I liked this book, and I didn't like this book. There are some books I never want to end. There are books so poignant, I have to set them down while reading just to process the words, ideas and thoughts behind a single page. Hillbilly Elegy wasn't, for me, one of these books. While Vance's story is interesting and full of larger-than-life characters, it never pulled me in or made me care about any of the people he wrote about, including Vance himself. I felt, reading Hillbilly Elegy, as if I was watching an episode of Jerry Springer. Some of the details were stunning and horrible; most of them have become shockingly mainstream. Perhaps I have simply been desensitized to this sort of tale because we've all heard it time and again. Facebook is littered with stories of abuse and neglect from parents or society. I can hardly pull up Yahoo's front page (which I do for email purposes) and not see a shocking headline: heroin addicted parents passed out in their car, their two-year-old in the backseat alone; small children die of starvation because their parents died of an overdose and nobody knew; CPS missed all sorts of red flags from overworked, under qualified case managers and something horrific happened as a result. The list goes on.
So, I wasn't shocked by Hillbilly Elegy. More than that, I just never saw any of the characters as more than flat or one dimensional in an all-too-familiar story. Vance gives too few emotional details to draw me into the characters, which resulted in a lack of connection. You could argue that my connection to the characters isn't the point of Hillbilly Elegy. The point of the book is to explore the limited options, poor choices and fading hope for a large segment of the American population, but for me, it was all too detached to feel like anything more than a very long news piece, written at least by a person who'd lived it. I think there is a certain detachment one experiences from childhood trauma like the stuff Vance lived and writes about; he speaks to this in the book, the idea that not reacting is a reaction of its own. I relate to and identify with that, as a person who experienced some of what Vance writes about myself, as a kid. There is no way to logically deal with much of life (particularly as a kid), so there is a self-protection element of detaching that enables people to weather the storm. While I understand this, I think it was this exact detachment I felt as a reader. Mary Karr writes a lot about perspective in terms of memoir, about the distance necessary to tell a story like Vance's with emotion and empathy. I'm not sure Vance is there yet, but that's a big statement to make from a woman who has never written a memoir herself.
There was quite a bit I did like in Hillbilly Elegy, however. Vance has a certain freedom from which to not only write about the story of hillbilly life (since he lived it) but also offer social commentary on the subject from a first-hand perspective. That is refreshing. It's hard to hear about the plight of any segment of the population from people who are viewing it from the distance of never having lived it. Vance has lived it. Because of that, he sometimes has pretty blunt verbal lashings for his own people and a take-no-prisoners attitude about what might make it better. He writes concrete sentences about what is wrong with a swath of American society that doesn't include such softening tactics as 'may be' or 'possibly.' Vance doesn't have to include a caveat in every sentence or conclusion, which is what I found most compelling about his book. The story is real, and his thoughtfulness and reflections are equally real. While the writing didn't stand out to me as a masterpiece of emotional connection, Vance's message is unique in that he is at least qualified to offer it. I didn't realize, until I read Hillbilly Elegy, just how off-point and warped so much of social commentary has become, in large part because it is created by people outside the proverbial circle.
I will say, I was drawn to this book and read it in a few days, which is a pretty big deal for me. I so often start a book and then dread continuing. The subject itself is fascinating for someone compelled by social issues, cultural anthropology and the psychology of a significant population of Americans. I'm not sure how it answers the questions raised by the presidential election. I'm not just saying that to be glib, either. But I have a serious distaste for talking politics, so I'll leave it up to the individual reader to determine if that is even relevant in reading Hillbilly Elegy.
The final thought I have about the book is a somewhat confusing one. What makes the book compelling is Vance's first-hand experience and thoughts on a subject that grabs so much of our attention but that we, as a society, feel ill-equipped to address. There is poverty, addiction, hopelessness, apathy, desperation, frustration, anger and detachment in this book. I think there is much of that the world, not only in America (obviously). There is also love, loyalty, laughter, head-shaking mirth and color. There is a lot of color.
But there is, in the end, a lot of confusion about what to do. The book asks the questions and, at times, offers possible solutions, but the overwhelming sense is this: what can we do to address all of this hopelessness, confusion, desperation, addiction and apathy? Even Vance can't answer that. It's a feeling we all know too well. We see the problems, but we can't grapple our way to the answers. Hillbilly Elegy left me with the feeling I've had so often working in aid/development and even education: the answers have to be organic and have to come from within. No matter if we are working on water security in Africa or food security in America, the solutions have to start from within the population needing the answers.
But what does that leave the rest of us to do?
Has anyone read Hillbilly Elegy? I'm curious to hear thoughts and know how everyone else experienced the book.


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Discussion in 'The Georgia Club' started by Square Dots, Apr 5, 2009.
Does anyone know of any LE dealers in the Augusta area? Thanks
My understanding is that the three LE dealers in Georgia are:
Clyde Armory in Athens/Bogart
Smyrna Police Distributors
Ed's Public Safety in Henry County
Nope none in Augusta
Ed's Public Saftey is a nice place and about an hour and 20 mins from Augusta. I was there Friday and they had a large selection on hand.
That's what I was afraid of. Thanks for the info. I will look up Ed's. Might be worth the trip for the discounted price.


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Even the gods make way for progress. Waghdev, who guards Purushwadi village in Maharashtra's Ahmednagar district from wild animals, and the tribal goddess Zakoobai, who, villagers belive is so powerful that she upturns idols of Maruti into the river, have now learnt to share space with new gods like Shiva, Ram and even Nirankari Baba.
Off the Ghoti-Sangamner highway, a winding road leads to the remote lair of Pur Oonch Wadi (Hamlet on the hill). Here Dattatraya Kondar, or "Dattaji", 24, in an XLRI Jamshedpur tee and jeans underneath his kurta-dhoti, welcomes guests with a tray of kumkum and hibiscus. "Welcome to Purushwadi. I am your guide," he says in rehearsed Hindi. Then, he cranks a rechargeable lantern and guides us in the fading twilight down hilly paths, passing by the river, the fields and the huts of the 109 families of his tribe.
The hosts: The villagers of Purushwadi pull out all the stops to welcome guests. Abhijit Bhatlekar/Mint
In the pitch dark, they tell you tigers come. They make you switch off your lantern, close your eyes and reopen them to get accustomed to the dark. The blanket of shooting stars makes you forget the toilets are still primitive here. Pitch a tent under a banyan tree. Only in the morning do they let you know you were sleeping on a 200-year-old ancestral grave.
The Watershed Organisation Trust (WOTR), a non-governmental organization (NGO) for water conservation, had been working in Purushwadi for five years when Grassroutes won a grant from WOTR and registered itself as a service-oriented private limited company (Grassroutes calls it an "UnLtd" company)—in 2006. For local boys like Datta, who tried to be a courier boy in Mumbai eight years ago, it meant not having to leave the village in search of employment again.
An XIM Bhubaneswar class field study recently indicated Purushwadi's average household income rose 20% over a two-year period since 2008. Grassroutes invests Rs 17 lakh and two-three years to make a village tourist-ready—setting up toilets, tents, training villagers. Then, it markets the village to tour groups. Grassroutes has three villages in Maharashtra—Purushwadi, Walvanda and Shiroshi.
Inir Pinheiro, a founder-member of Grassroutes and an awardee of the Karmaveer Puraskaar for Social Justice and Citizen Action 2008-09, explains why they follow a "for profit" business model: "For sustainability, you must have environmental, social and financial sustainability. Village systems are socially stable because the community is at the centre.
Environmentally, the community ensures people don't tear down fields to put up hotels. Financially, ventures depend on grants. Once a grant dries up, you have to make sure the tourist returns to ensure this sustains."
Forty-five per cent to 50% of the money from each tourist goes towards paying "service providers" such as guides, housekeepers, and cooks—functions villagers share on a rota basis; and into a corpus fund for the village. The rest of the money pays Grassroutes salaries, marketing costs, etc. "The fund is in a joint bank account with our NGO partner (WOTR)," Pinheiro says. Villagers can apply for the fund for anything except religious or political purposes. It also ensures that villagers who don't participate directly in tourism benefit.
A village committee ensures the villagers have a say in all processes—including deciding their rates. "I get Rs 60 as a guide, Rs 50 is my per day labour when I work in the fields," Dattaji explains.
The village police liaison, "Police Patil" Poppatrao Kondar, 50, a committee member, says: "The biggest benefit has been to women. They also get money for their cooking now—Rs 3 for tea, Rs 10 for breakfast, Rs 30 for a meal."
Some villagers stayed aloof. Ramnath Kondar, who once opposed it, now leases his house to Grassroutes for tourists to sleep in. "Everyone understands money," Pinheiro says. The corpus fund is now Rs 1.45 lakh over four years, Police Patil says.
Komal Kondar, 14, a class IX student, wants to be an engineer. She runs to meet the women bankers and engineers on holiday. "I can also be like them," she chirps. An old man demands a lantern of his own, and wants Pinheiro to buy one for him. "What do you think? I don't have Rs 1,000?" he asks, offended. Thanks to the tourists, now they do.
Grassroutes: www.grassroutes.co.in


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John Sinclair landed 'bop' in Amsterdam,
to face news that he only has
4 days left
on his tourist visa inside the E.U zone
so a new plan was put together.
48 hours with John Sinclair in
friday afternoon, november 8th
i meet John in Hempshopper
Sid on phone with Dutch
ministry exploring possibility John
can stay in Holland, where he wants
to stay, just a little
while longer
after some deliberation on the issue
John and i head out
for lunch
wonton soup and China tea,
John now feeling twenty times
better fed
after long journey from Detroit
soon seated in cafe 420, expresso in hand and
reefer, John passes along 'Satori in Paris'
and 'Pic', two novels by Jack Kerouac.
meanwhile i look up traffic control:
a UK company that deal with visa
and passport
concerns for artists
John and i leave the cafe
in a bike-taxi-quad
over to the
sensi seed palace in the red light
seedy indeedy
the viper viped'
i left to greet a friend visiting from
I spent the night out,
and a rare late night chin wag
with my
mucka from home
and Caleb
saturday i awoke and my mate Scot
had gone
i quickly left for a meeting with Sid and John
at the Palace,
John got ticket to leave by rail to the UK
the following day,
I head off to work at the cafe,
busy shift and John sits and writes,
and makes another radio show
for Radio Free Amsterdam,
Number 521: featuring Caleb Selah
of the 'Fuck You SOund', and music by
John himself, and the Rebirth Brass Band.
John stays chillin' for my shift
8 hours, writing, talking, reading
the International Herald Tribune
smashing the crossword puzzle
to coherence
sunday morning i meet John
he turns over his weed to me
and 'The Town and the City' by Jack Kerouac
and we take another bike-taxi back to
John sits in the clear light
peacefully enjoying his coffee and joint
nobody gets hurt.
14.45, John and i leave by cart
to the station, pick up a snack
paper, off he went
to Brussels
and then to the UK.
I head back to apartment
to meet Caleb, smoke anoint
Caleb leaves for the UK
high as fuck
following monday
I discover traffic control only operate
outside of E.U zone
our last options indicate
a work visa
to be issued in such
shrt time period.
and so we break the news to John
he chuckles and takes the news well
looking on the bright side
as always.
See you at the show on the 24th!
--Steve Fly
Just A Little While To Stay Here: John Sinclair Radio Show 521
Episode 521 is emanating from my regular stand at the 420 Cafe in Amsterdam, but contrary to my plans I'll be leaving here tomorrow to stay in London until (and after) our Cannabis Cup party at the Akhnaton November 24. I learned when I arrived in the Netherlands yesterday that I've overstayed my welcome in he European Zone for the current six-month period that started with my arrival for the summer on June 21, so right now I've got two days left of the 90 allowed and I'll have to return in two weeks to enjoy them here. Meanwhile I'll be holed up at housedem.com in London, home of The Fuck You Sound, thanks to Caleb Selah & Co. Tonight at the 420 I'm playing music by the ReBirth Brass Band, tunes from Detroit by the Planet D Nonet, the Flying Crowbars, the Butler Twins, and Laith Al-Saadi; music from Holland by the Misha Mengelberg/Piet Noordijk Quartet; tunes I picked up in Portland ME by Angelica Sanchez and Melanie De Biasio; three cuts from my forthcoming Ironman album called Mohawk with Steve The Fly; and a send-off by Glen Andrews & the Lazy Six.
The John Sinclair Foundation Presents
420 Café, Amsterdam, November 9, 2013 [20-1345]
[01] Opening Music: ReBirth Brass Band: Just a Little While to Stay Here
[02] John Sinclair Intro, ID & Opening Comments
[03] Planet D Nonet: Island in the Sun
[04] Flying Crowbars: Shake Me
[05] Butler Twins: The Butler Jump
[06] Laith Al-Saadi: What It Means
[07] John Sinclair Comments
[08] Misha Mengelberg/Piet Noordijk Quartet: Sugar 'n Spice
[09] Angelica Sanchez: 514
[10] Melanie De Biasio: The Flow
[11] John Sinclair Comments
[12] John Sinclair: Bloomdido
[13] John Sinclair: Mohawk
[14] John Sinclair: Carolina Moon
[15] John Sinclair Closing Comments & Outro
[16] Closing Music: Glen Andrews & the Lazy Six Over In The GloryLand
Hosted by John Sinclair for Radio Free Amsterdam
Produced, recorded, edited & assembled by John Sinclair
Executive Producer: Sidney Daniels
Sponsored by Hempshopper & Ceres Seeds, Amsterdam
Special thanks to Sidney Daniels, Steve The Fly, Caleb Selah, and Sensi Seeds
© 2013 The John Sinclair Foundation


text:
Right now is actually the moment to assist someone you love who possesses a substance addiction issue. You could certainly not let him simply eliminate himself along with drugs and alcohol, but if you allow him to continue on the path he gones on, you recognize he will not survive. This is tough to persuade an individual they need aid, so instead obtain help coming from others you recognize are his close friend. You must combine all his friends together to fight a power as powerful as addiction. The greatest trait you can do is actually to not surrender. Too many individuals give up on their drug abuser buddies and also leave all of them to destroy their personal lives, instead of ruining the lives of individuals around all of them.
Pick The Most Reliable Drug Rehab Facilities In Beaverton AL Here
Missing teeth could be just one with all the implications of medication addiction. When a routine takes control of your lifestyle to such an expand that you simply right now not care worrying how or where you reside, you have actually got undeniably come under a pit which could be tedious to go up out of.
Having the assistance of your friends and also household are going to provide you with further inspiration to receive healthy as well as well-maintained. Join an assistance team, it will assist you link with others that possess and are currently going by means of the same things you are.
I consistently possessed a "push God to the limit" type of relationship along with Him. , if I had not been delighted along with The lord I wasn't reluctant to allow Him recognize what I thought of Him or His plan.. Down with the years I would certainly "have it out with Him" as well as would certainly point out points to God that "good" Christians just weren't supposed to claim to The lord, till this related to the time I ultimately informed Him in order to get the heck out of my life … FOR LIFE!
What Is Actually A Rehab Center For?
Rehabilitation centers that claim all sort of results rates may be complicated. The essential to learning which ones make good sense is actually to view exactly what they consider to become an effectiveness. In other words, do they feel that somebody merely accomplishing their course is actually a results? Perform they think a person mosting likely to conferences (whether they are actually well-maintained or otherwise) is a results? Or even worse, do they really placed them on some sort of addicting replacement medicine and still think that is actually an effectiveness? An instance of the could be methadone upkeep. If they are not on heroin any sort of even more yet are actually consuming and also having methadone some courses could still take into consideration that an excellence.
The Beaverton Alabama Drug Treatment Centers You May Trust In
The lengthy phrase impacts of going to a medicine therapy facility are really real. That is actually very easy to rehabilitation if you're divided coming from obtaining the medications and also certainly not in the exact same social conditions.
Now is actually the time to create a trip at your living fourths. The possessions as well as the contents of the client will be actually contacted utter care. All the possessions will be locked or destroyed, relying on the legal policies. You can easily opt for your very own garments, but a lot of the rehabilitation centers possess their certain dress regulation.
To Conclude
The above solutions, it is strongly encouraged for any individual struggling with drug dependency to go to a medicine rehab procedure. Locate a great one today and offer drug dependence goodbye.


text:
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Starting in January 2013, this page will be a one-stop shop for all Butthead and anti-smoking materials. Whether you're looking for a facilitator guide to help you deliver an in-class interactive presentation, or new ways to link Butthead activities to lesson plans and the NWT curriculum, this is the place to look!
NWT Tobacco Education Resources


text:
Drawing doodles is a seriously underrated lucrative business.
SEED FACTORY - For once and for all: a definitive homage to 'Ever' and his endless set of illustration skills.
HELLO AGENCY | DECATHLON - In good shape.
DECODERS | IDEWE - A street smart map.
CHRISTEYNS - Squeeky, milky clean.
ART COLLECTION DEXIA BANK - St. Petersburg goes unorthodox.
GARABATTAGE MAGAZINE - Drama queen strikes again.
ART COLLECTION BRYS-CALLEWAERT - It's a smooth operator.
A series of literary supplements for leg fetishists, grey matter masters and misunderstood maestros, to mention a few.
A sensible sign for all of Mother Nature's critters, creatures, carnivores and cutie pies.
Hic Sunt Leones. In comic configuration.
How do you make residents aware of free parking right in front of their door? With well-built and well-hung landmarks!


text:
Birthday Wishes is Book 1 in The List Series is intended for an adult audience only and contains mature themes, including graphic and explicit sex.
"When you said you wanted to work on your birthday wish list, I thought you meant it metaphorically, not literally." He led me smoothly through a song change. He was so comfortable on his feet I couldn't help but wonder what it would take to unravel him just a bit.
"Nope, no metaphors here."
"Had I known, I would have wrapped the package a little better."
I looked at his crotch reflexively and pictured his cock with a ribbon and bow tied around it. "It's not too late, you know."
He placed a finger under my chin and tipped my head up. "Your mom is watching us." He was totally laughing at me. Busted checking out my date's goods by my mom. Unfortunately, my eyes needed to stay above the waist for the rest of the evening.
"Fabulous. One more thing for her to ask about."
"I created family problems by coming here?"
"Problem is too strong a word, but since my divorce. . ." I let the sentence hang. How could I explain my mom's interest without making her look like a pushy nut job? In fairness, she was a little bit of a nut job, but I think that was just my biological clock ticking. I couldn't hear it yet, but somehow it'd tapped into my mom's system instead of mine. Children were not high on my list of concerns, but grandchildren topped hers.
"Until your brother explained, I thought you were the one who gave me the address."
"I figured. Ricky likes to play. He didn't mean any harm." I gave my baby brother a raft of shit at any available opportunity, but just like any older sister, I'd be damned if I let someone else judge him harshly. It was my job to defend.
"He cares about you."
"Mmm." What else could I say? Admitting we liked one another is not something Ricky and I would ever do willingly. "You should tell me something about yourself," I said, "like what you do for a living." My mom was going to ask and I couldn't just say I don't know, Mom. I want to fuck him, not marry him.
All Wrapped Up is Book Two in The List Series and contains mature adult themes, including graphic and explicit sex.


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See our child Kiara is socializing with all like there is no tomrrow ,'but …you are scared about her life turning upside down hmm…is′nt it?' Vasu questions Meera as he kisses her medulla oblongata with passion .
"Yes …sweetie with upcoming Shirley ′s treat of ruining our happiness by bring our past back to present " Meera responds stiffly .
Ah !My sweet jaan do not bother much as our children Kiara and Tejas have seen our past and their behaviour towards us , now its their time ie our past to worry about nemises and their reputation ,so come up here and lets us now enjoy food at Destination Meera where her hubby and children are waiting for her to join for a fun filled lunch .., Vasu stats and Kaira….he ways at her .
With Lots of Love ,
Author N


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The St. Louis Cardinals closed to within 3 1/2 games of the National League Central lead on Wednesday with a 5-1 win over the front-running Houston Astros. The Cardinals also maintain a three-game lead in the wild card race with the win. Mark McGwire homered twice to lead the offensive attack in the win. Darryl Kile (15-10) pitched seven shutout innings to earn the win for St. Louis. He struck out ten.
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Beginning September 1, the Real Estate Council of B.C. has stipulated that all new applications for licensing by individuals must include photo identification.
The Council amended its general bylaws last June to require photo identification after broker Indeep (Ted) Singh Aulak was caught writing a real estate exam for his friend, Ryan Gurinder Rana of Ryan Rana Personal Real Estate Corporation.
He swore a false affidavit attesting that he was Rana and applied for registration in the Trading Services Applied Practice Real Estate Course on behalf of Rana.
According to FICOM's investigation, both the RETS Exam and the Mortgage Brokerage in British Columbia Examination are administered by the Real Estate Division of the Sauder School of Business, at the University of British Columbia.
On April 15, Rana admitted to the Council that he did not write the RETS Exam and that someone else wrote the RETS exam for him.
Rogers said that it was in the public interest to make a summary order because, among other things: "Mr. Aulak's actions are particularly concerning given the short period of time in which he has been registered as a submortgage broker. In addition, Mr. Aulak is active in the real estate industry and maintained a professional relationship with Mr. Rana by listing one of his properties with Mr. Rana, whom he knew was not properly licensed." – CINEWS


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Top 10 Funny Travel Books For Armchair Travel (1)
In the summer, I-10 is clogged from Mississippi to Florida with vacationers visiting the beautiful sugar sand and emerald water beaches alongside the Gulf Coast, particularly the Florida Panhandle and Orange Beach, Alabama. Don Boles, who was dwelling in California back within the day, began manufacturing all aluminum, all riveted light-weight journey trailers shortly after the end of World War ll when he realized that there was a substantial amount of interest in such automobiles. His trailers were put on the market lengthy earlier than the pricing for RVs grew to become costly, however paradoxically, there isn't a brand in the marketplace at this time that may match his for quality and sturdiness. Packing cubes help business vacationers (and leisure vacationers, too!) pack rather more effectively, letting them match extra clothes into each suitcase. But there's nothing that says eighty's nursing residence greater than this powder blue & pink speckled pattern so its gotta go.
If you wish to stay in one in all their 40 rooms and suites (scattered throughout the village), e book by means of your Virtuoso travel advisor. You present your travel recommendation in an exquisite reader-friendly vogue, providing a pleasant stability of pros and cons, and providing frugal alternate options to sure things we right here in America take without any consideration. This article offers you some tips that will aid you to know what you might have to pay once you own your journey unit. I actually have never discovered them to be units I would want to travel in, although they are easy to tow, and so on.
Examples of reasons for issuing a Travel Alert may include an election season that's certain to have many strikes, demonstrations, or disturbances; a well being alert like an outbreak of H1N1; or proof of an elevated risk of terrorist assaults. You are appropriate: travel is so much easier now, as well as details about the world that we're not acquainted with.
If that is what you want them, it is best to verify among the silk travel blankets and cashmere journey blankets out there. If you determine to stay the evening, there are 12 motel rooms, 6 yurts, 3 teepees, RV hookups and camping websites accessible. Thus, travel units might be safer, extra durable, better constructed and designed, and extra snug than they are now. Some folks see the need for change to feel alive and see Travel Nursing as a chance to do that. And as if things couldn't get any better, we finished the night with gelato at Giolitti and all 5 New Yorkers boarded our private classic Bici & Baci vespas to tour Rome by night. And yet one more function that makes this memory foam travel pillow so special is that the included water repellent storage bag compacts the pillow right down to 1/4 its size! Here are a couple of suggestions we have gathered through personal expertise and the recommendation of different journey specialists.
I love travelling and this is mostly a protracted distance journey for 10 to fifteen hours. Although my wanderlust has since widened my travel goals, I hadn't fully appreciated the restrictions of the 40 year previous tin and birch veneer charmer. Tucked into the guts of the world's greatest mountains, Kathmandu hosts an absolute banquet of 7 UNESCO World Heritage websites providing trekkers to the Himalayas a singular peek into a unique culture. Motor homes and campers are fantastic to personal and journey in, but before you purchase one, it's worthwhile to be certain you will be able to afford to restore it. In instances of world turmoil, the journey industry is usually the first to be adversely affected.


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Breaking news! I'm proud to announce I received the inaugural "Legacy Award" for being a "positive force" in my community to improve parks and recreation and for my leadership of Friends of Des Moines Creek and other volunteers for nearly two decades. Please read the article here: LegacyAwardArticle August 2017
As I walk or jog along Des Moines Creek each week, I am truly thankful for all the volunteers who have given their time to help improve our natural areas. I couldn't do it alone. Though the award was presented to me, it was earned by all of us.
Friends of Des Moines Creek
Since 1999, Laura has led Friends of Des Moines Creek and partnered with community volunteers to restore fish and wildlife habitat and maintain and protect miles of Des Moines Creek that flows into Puget Sound.
Past projects along Des Moines Creek include:
- Removing invasive English ivy, Himalayan blackberries and other non-native vegetation (See how volunteers remove ivy on steep slopes here):
- Planting native plants;
- Mulching around installed plants; and
- Installing fences to protect the creek buffer.
To inquire about ongoing volunteer opportunities with Friends of Des Moines Creek, or to suggest a project with your own group, please email email@example.com. Please include "Volunteer" in your subject line.
Bird Lovers, click on the links below to discover what birds were observed by Laura and volunteer Sharon Cormier-Aagaard along Des Moines Creek:http://ebird.org/ebird/DesMoinesCreekBirdsSept2015 ; and http://ebird.org/ebird/DesMoinesCreekBirdsJan2016.


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The fava bean is a springtime legume, it is delicious and very popular in Rome, but in the ancient times it was often connected with superstitions and with the world of the dead.
The fava bean inspired horror to the Pythagoreans because the black stains on the fava flowers were believed to be a sign of the presence of dead people souls; the Egyptians avoided the contact while the Romans offered them to the dead as a gift.
The reasons of this bad consideration are in the botanical characteristics of the plant and in the difficulty of digestion. The plant is the only one with a stem that comes out from the ground without any knot so it was believed to be a sort of thread of communication with the underworld, used by the dead to take possession of the souls of living people. The fava been is in fact a little bit hard to digest giving a sense of psychic and physical heaviness.
It can also be mortal and provocate an anaphilactic shock in people affected by favism, a quite diffused allergy in Southern Italy. All this of course increased the fame of the fava bean as an impure and dangerous food.
Still nowadays we keep memory of this funeral aspect with some biscuits prepared for the feast of the dead falling on November, 2nd: though they're made with almonds, they're called "The dead's fava". The best period to eat this highly proteic food is May/June when you can find it fresh and eat it raw with Roman Pecorino cheese or in a typical Roman recipe called "Vignarola".


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Counselling & Psychotherapy Services - areas we work with
Barnsbury Therapy Rooms, in Islington North London, offer counselling and psychotherapy services for a number of issues including depression, anger management, anxiety, bereavement, sleep problems and stress. You can also take Yoga classes or explore Sophrology and Mindfulness. To find out more click on the links to the right of this page. You will also find out more about our therapists' particular interests and specialisms from their individual profiles. Please take a look at the directory of counsellors and psychotherapists in North London.


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Happy Hour #144: Sarah Harmeyer
Podcast: Play in new window | Download Sarah Harmeyer is my guest for episode #144 of The Happy Hour. Sarah is the founder of Neighbor's Table, a concept born from her personal passion… Read More
Around the Web
Podcasting weekend + new shows
This weekend I attending a conference all about podcasting. It's the first conference that I've attended in a long time where I didn't have some sort of responsibility, and it's the first… Read More
Around the Web
Uganda here I come.
"I'M GOING TO AFRICA" said in my biggest voice all while smiling from ear to ear!!! Africa here I come. In a few weeks I'll board a plane for a far off… Read More


text: Career Center Employers? Post Jobs and More Job Seeker Sign In Home Jobs Your Profile Resources Your Account Job Seekers Sign In New Job Seeker? Sign Up Overview Your Saved Jobs Your Job Alerts Your Profile Your Documents Your Applications Help Welcome to AAPD Career Center Search Browse Explore Your Job Alerts Your Saved Jobs 0 Return to Search Results Public Safety Officer California College of the Arts savedJobs Apply Now Continue To Application The application opened in a new tab. By using this feature you agree to our Terms and Conditions and Privacy Policy. Details Posted: July 1, 2017 Location: Oakland, California Salary: Open Type: Full-time - Experienced Employment Type: Full-time Organization Type: Higher Education Institution Public Safety OfficerCalifornia College of the ArtsMAIN RESPONSIBILITIESID and Access System FunctionPatrol FunctionMINIMUM QUALIFICATIONSLocationApplication InstructionsApplication DeadlineEEO firstname.lastname@example.com full application instructions and position description, visit https://cca.wd5.myworkdayjobs.com/en-US/CCA/job/San-Francisco/Public-Safety-Officer_R502356 Copyright 2017 Jobelephant.com Inc. All rights reserved.Posted by the FREE value-added recruitment advertising agency jeid-1e23d8d615a449599b6e2caa26ec6220 Internal Number: 1037835 Send Me Similar Jobs By Email About California College of the Arts Founded in 1907, California College of the Arts (CCA) is an independent art college with 21 undergraduate programs in architecture, design, fine arts, visual studies, and writing. The college also has graduate programs in architecture, comics, curatorial practice, design, design strategy, film, fine arts, visual and critical studies, and writing. Through these programs, the college has created a d...ynamic and interdisciplinary environment. Diverse cultural perspectives enrich the CCA community and the college believes that continuing to expand the horizons of the students and faculty is essential to achieving its educational objectives. With campuses in Oakland and San Francisco, CCA currently enrolls 1,950 full-time students. Show more Show less View All California College of the Arts Jobs More Jobs Like This Sales Rep / Outside Sales / Territory Sales Terminix Austin, Texas Today Nurse Manager, St. Francis Primary Care, Bilingual Spanish/ English, FT Nemours duPont Pediatrics, Saint Francis Wilmington, Delaware Today Hockey Operations Coordinator Rochester Institute of Technology Rochester, New York Today Back to top Help is on the way! We're sorry you are having trouble applying for this job. Please try loading this job using the following link before submitting your help request: Name: Email: Please provide details: Submit Help Request Error Close Continue Powered By


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Emergency Medicine Clinicians & Instructors
Justin Belsky, MD, MPH
Dr. Justin Belsky completed his Emergency Medicine Residency at Henry Ford Hospital followed by a Research Fellowship studying sepsis at Massachusetts General Hospital. His research focus is on the early identification of sepsis through biomarkers and analysis of the microcirculation.
Jessica Dara Bod, MD
Dr. Bod is the Associate Director of Medical Student Education and the Director of the Emergency Medicine Sub-internship.
Kevin Burns, EMT-P, PA-C, DFAAPA
Assistant Clinical Professor of Emergency Medicine
Co-Director, YNHH Emergency Medicine PA Residency
EMS Coordinator
Ryan F Coughlin, MD
Instructor in Emergency Medicine
Clinical Interests
Disaster Medicine; Emergencies; Emergency Medicine
Ryan F. Coughlin, MD is a Clinical Instructor in Emergency Medicine. He serves as associate EMS Fellowship Director, and residency EMS rotation coordinator. Dr. Coughlin is the medical director for the Yale-New Haven Sponsor Hospital Program paramedic field instructors. He is also a member of of the Sponsor Hospital Area Response Physician (SHARP) Team, the only state-certified EMS physician response team in Connecticut.
Dr. Coughlin has specific interests in tactical emergency medical...
Ambrose H. Wong, MD, MSEd
Instructor in Emergency Medicine
Director of Simulation Research
Associate Fellowship Director, Medical Simulation
Ambrose Wong MD, MS-HPEd, Director of Simulation Research at YCMS, is a board certified emergency physician with an interest in using healthcare simulation to improve interprofessional collaborative practice and patient-centered care.
He received his Bachelor of Science from the University of British Columbia in Microbiology & Immunology in Vancouver, Canada and attended Washington University School of Medicine in St. Louis, Missouri. Ambrose obtained his Emergency Medicine residency training at...


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Help Us
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About the Pug Rescue Network
Founded in 2004, The Pug Rescue Network maintains a vision of saving Pugs in troubling and life threatening situations. Thanks to the dedicated work of our volunteers, we are able to locate, retrieve, and eventually place rescued Pugs into loving new homes.
The Pug Rescue Network (PRN) was launched in September of 2004 by a small group of individuals who have spent years working with other rescues. After seeing the tremendous difference volunteers could make in a rescued pets life, these individuals had an idea. After spending considerable time doing research as well as participating in extensive dog training and behavioral seminars, the concept was drawn and the foundation of the Pug Rescue Network concept and mission became a reality:
Embrace the neglected, forgotten, and displaced Pugs with compassion, providing hope through education and awareness, helping them by finding their way to a permanent loving home.
To ensure each Pug is given the best chance at finding a new home, all Pugs turned into the Pug Rescue Network are evaluated for temperament and overall disposition. They are then placed into our Foster Network of private homes - we do not have a facility for you to visit.
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How to Select the Best Coolant
Selecting the right coolant isn't as difficult as it seems. Engines produce heat during the combustion of fuel. The heat can melt some engine parts, thus causing damage to it. Hence, you will need a coolant system.
The coolant system will keep the engine from overheating. It's a mixture of water and anti-freeze. Coolants transfer heat to the radiator from the engine. They also protect the automobile from freezing in places with temperatures below freezing point.
A coolant consists of additives, deionized water, glycol and inhibitors. Glycol raises the temperature of the coolant. There are 3 types of coolants. Before buying one, you have to know one that suits your engine. They include Hybrid Organic Acid Technology (HOAT), Inorganic Acid Technology (IAT) and Organic Acid Technology (OAT). These 3 types of coolants have different colors, inhibitors as well as additives.
You shouldn't use more than one type of coolant in your car. This can damage your engine. Never combine a high-grade coolant with a low-quality one since it doesn't increase its service life, Always get a coolant of the same kind as the one in your engine. Utilize refractometers, test strips and hydrometers to determine if there are inhibitors in the coolant.
Refer to the car's manual. The majority of automobile manufacturers use a certain type of coolant in all their cars. The automobile's manual will enable you to know the type of coolant you should pick. Find your automobile's inhibitor rating. If you've lost the manual, visit the website of the manufacturer. You'll get the details you need.
The quality of an item increases its popularity. The same can be said for coolants because popular ones prevent corrosion and provide good protection to engines.
An important factor you need to consider is the service life. This refers to when the coolant should be removed from the vehicle's system. When choosing a coolant, consider how frequently you'll need to flush your car's cooling system.
Take you budget into consideration. This is the amount of cash you're ready to spend on the coolant. Go for the best quality coolant that you can pay for.
If you're not sure about the best coolant, ask an expert to assist you. You can ask about coolants from the mechanic who repairs your car. He's likely to have a few tips that can help you pick the right coolant for your engine.
Some coolants should be mixed with water, while others should be used as they are. To make sure you have the correct concentration, you have to stick to the ratio.
Picking the wrong coolant can lead to serious engine problems. Buy a coolant with the right additives to prevent your cooling system from corroding.
What Almost No One Knows About Fluids
The 9 Most Unanswered Questions about Resources


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A few months ago I was sent some wonderful superfood treats- blueberry powder from the amazing girls, Eve and Anna, at Arctic Power Berries and some moringa powder from lovely Lola at Aduna. I'm very lucky and so grateful to both of these inspiring companies.
Now we get to the smooth and creamy vibrant blueberry layer. Just like the Aduna team, Eve and Anna make the purest raw berry powders, free from added sugar, gluten, preservatives and added flavourings, non GMO and vegan. They have a beautiful selection on their website so check it out. As they inform their readers, 'Wild arctic berries are naturally rich in vitamins, antioxidants, nutrients and fibre. They grow and mature under the midnight sun, absorbing the goodness from their clean and unpolluted surroundings'. Just 1 tsp of wild berry powder is the equivalent to a handful of berries. Find out all about how amazing their blueberry powder is HERE Combining the powder with local raw honey really lifts the blueberry flavour, and in turn, then lifts up the lime in the divine mousse-like topping, finishing with the great crunchy base, you'll definitely be coming back for seconds of one of these superfood squares!
Matcha-moringa cacao-buckwheat-crunch base
60g pumpkin seeds
60g sunflower seeds
60g buckwheat groats
1 scant tsp matcha green tea powder
1 scant tsp moringa powder, I use Aduna
½ tsp Himalayan pink salt
20g cacao nibs
120g Medjool dates
2 tsp vanilla extract
3 tbsp water, preferably filtered
3 tbsp coconut oil, melted
Blueberry cream
200g cashew nuts, soaked with 400ml filtered water with ½ scant tsp Himalayan pink salt for 3-4 hours
130g/ml almond milk
80g raw honey
100g coconut oil, melted
4 tsp blueberry powder, I use Arctic Power Berries
6 tsp lemon juice
Avocado lime mousse
135g avocado flesh
170g natural coconut yogurt
35g coconut nectar syrup, blonde
25g raw clear honey
55g coconut oil, melted
Finely grated zest 1 lime
50ml lime juice
Start by soaking the cashew nuts for the blueberry layer.
To make the matcha-moringa base, line a 30cm x 20cm brownie tin with baking parchment. Place all the ingredients from the pumpkin seeds to the cacao nibs in a food processor and blitz a few times. Add the rest of the ingredients until everything comes together. Tip the mix out into your prepared tin and press it in until even and flat. It's helpful to put a piece of baking parchment over the raw base, and press the base out and smooth it with your hand. Freeze.
To make the blueberry layer, drain and rinse the cashew nuts thoroughly. Place everything in a blender and blend until completely smooth, stopping now and again and scraping the mix down. Pour over the base, spread the mix out and then bang it a few times on a surface, just to make sure that it's all level. Now place the tray in the fridge until the blueberry layer has set firm; this can take up to 4 hours so leave it overnight if you want.
For the avocado lime mousse, place all of the ingredients in a blender and blend until smooth. Pour over the firm blueberry layer and return to the fridge to set once more, which will take a few hours. When set, cut into squares. To make nice clean cuts, dip a sharp knife in hot water then dry it before cutting each slice; clean the knife in the hot water, dry and cut again; continue for the rest of the cake. I like to cut my cakes into about 3.5cm square pieces, but see what size you prefer.
Decorate with fresh blueberries, buckwheat groats, seeds and edible flowers (I use violas from my garden). The cakes will last in the fridge for a good 4 days.
Replace the raw honey with coconut nectar syrup if you are vegan.
- Henrietta from http://www.henscleancakes.com/


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Connect five or more robots in chains and watch them explode! Exploded chains give the energy that you collect and use to restore the RoboPlanet, which lies in ruins after a short circuit. The whole RoboCivilization relies on you! RoboSockets is a juicy mix of tetris, pipes and match 3 game mechanics resulting in the truly unique gameplay. It's as simple as genius and brings a breath of fresh air to the tried bejeweled-type games. The game introduces a whole RoboCivilization with its hierarchy, history and legends. Apart from brand new gameplay, RoboSockets boasts of classic for a quality title virtues: cheerful UI, elaborate story, intense graphics and a vast replayability potential. The game features: • Highly addictive gameplay • Challenging game modes • Memorable characters with exclusive anatomy • Explorable facts of RoboCivilization • Leaderboards and Achievements (powered by PapayaMobile) • Facebook integration
Downloads: 122+ (for Android)Report app for spam or abuse!
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Scat video tube. A young mistress is spitting, peeing and shitting hard into the tolet slave's mouth making him swallow it all.
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DeliaItaly is knackered. I know all you bloggerettes will miss me, but I'm off to the Tyrolese Alps for a week. Escaping from absurdly hot weather (partly responsible for my bad running); my ageing Dad; his tiresome careperson and this computer. See you all soon, be good and keep on running. I shall do so in the mountains Heidi permitting, xox delia
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, as President,
Trump - hoće li biti impeachment ili 8 godina drugačijeg predsednikovanja?
Posted 21 October 2017 - 16:28:46
Posted 21 October 2017 - 16:36:00
fake news
Congress mandated in 1992 that all assassination documents be released within 25 years, unless the president asserts that doing so would harm intelligence, law enforcement, military operations or foreign relations.
Posted 21 October 2017 - 16:52:21
Ne može bilo kakva šteta, mora biti značajnija od uskraćivanja prava javnosti da zna.
The Act requires that each assassination record be publicly disclosed in full and be made available in the collection no later than the date that is 25 years after the date of enactment of the Act (which will be October 26, 2017), unless the President of the United States certifies that: (1) continued postponement is made necessary by an identifiable harm to the military defense, intelligence operations, law enforcement, or conduct of foreign relations; and (2) the identifiable harm is of such gravity that it outweighs the public interest in disclosure.
Posted 21 October 2017 - 22:21:14


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Rubbish wildlife and ravenous ravens
Jody Bourton
Like the name suggests, cannon netting is a process whereby mini cannons are attached to nets. They are set up near spots where the birds are likely to land. Then, when the time is right and following a 3-2-1 countdown, the cannons are fired taking with them the nets and capturing the birds. Bingo! Well, that's the plan anyway...
It's a humane process and the birds are quickly and efficiently gathered then measured and ringed before being freed.
We had it all set up and ready to go. But wildlife being wildlife, the seagulls were having it their own way, and remained circling high above for the remainder of the day. In hot weather, we were left waiting and waiting with only the sweet smells and sights of people's unwanted refuse to look at. Nice.
And yet it was an experience I reckon everyone should go through. It certainly makes you think, seeing society's unwanted stuff - tonnes of it - and it still piling up day by day.
Not that the seagulls minded. Indeed there were quite a few other birds about too. Seagulls of various types, jackdaws, crows, buzzards and even some enigmatic ravens.
Ravens are intriguing birds surrounded by stories and folklore. You can recognise ravens by their jet-black colour, deep beak and wedge-shaped tail. But when you're close to them, the most striking thing about ravens is their size. They're huge and must be at least the size of a buzzard.
On Tuesday I was fortunate enough to travel to a raven roost on Anglesey. It was also a bit of a treat as on the way there I passed through the village with the longest name in Europe - Llanfairpwllgwyngyllgogerychwyrndrobwllllantysiliogogogoch. Like many Welsh folk, I'm often asked for a pronounciation when people find out where I'm from - so it was nice to see the place for real.
Unfortunately, the ravens weren't there en masse as it was daytime - and that's when they're out foraging. But it was an enchanting place with a few individual birds squawking above us.
Often associated with death, ravens sometimes get bad press. They almost became extinct in the UK but after becoming protected their numbers have increased. In fact, Newborough on Anglesey plays host to one of the largest raven roots in the UK.
Being part of the corvid family, ravens are very intelligent creatures. Recent studies have suggested they actually recognise facial features. It's also believed that they are able to communicate the location of food sources to each other plus a whole host of other surprising skills and behaviours.
While we were there I was shown a raven food pellet. This is the material that they can't digest and so cough up in a ball. Depending on what the bird has eaten, the pellets can look quite different. The one we found looked a bit like a Weetabix which showed the raven had eaten lots of seeds and cereal-type foods.
So that's certainly one to look out for. If you see a Weetabix in the wild it's probably not someone's unwanted breakfast but a sign that ravens have been close by!
You can find out more about some the stories surrounding ravens here. Use the comment form below to let me know what you think.
In the meantime, here's my pick of this week's wildlife web: