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"Dah dah dah, dah daahhh ... Mistaaaaaaaake," hums Jim Gaffigan as he recreates the well known McDonald's theme tune "I'm Lovin' It," during a segment of his stand-up performance, Mr. Universe. Well, Long Beach residents, hold onto your BigMacs and leftover McDonald's fries, because beloved actor, Grammy-nominated comedian and New York Times bestselling author will be gracing Long Beach's Terrace Theater.
Gaffigan's OBSESSED tour is a rampant take on our culture's addiction to food and the shame of eating like a fatty. Gaffigan will be slamming seafood, discussing "the American dream," and lamenting that he'd rather eat an entire pint of ice cream than live long enough to see his daughter get married, "Don't you die in 18 years there's an awkward party you have to pay for!" screeched Gaffigan during "Weddings".
Tickets look a little pricey—$62.50 for orchestra—but it's probably worth the dough for more than a few hearty laughs about the reality that is food in America, and everything it has to do with. Note: don't be fooled by Mr. Gaffigan's homepage; the Long Beach stops are not sold out as his website claims.
OBSESSED hits the Long Beach's Terrace Theater this Thursday, December 4 at 8:00PM and Sunday, December 7 at 7:00PM. Tickets can be purchased here for the Thursday night performance and here for Sunday night's performance.

subset: commoncrawl


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Reactive oxygen species (ROS) signal vital physiological processes including cell growth, angiogenesis, contraction, and relaxation of vascular smooth muscle. Because cytochrome P-450 family 4 (CYP4)/20-hydroxyeicosatetraenoic acid (20-HETE) has been reported to enhance angiogenesis, pulmonary vascular tone, and endothelial nitric oxide synthase function, we explored the potential of this system to stimulate bovine pulmonary artery endothelial cell (BPAEC) ROS production. Our data are the first to demonstrate that 20-HETE increases ROS in BPAECs in a time- and concentration-dependent manner as detected by enhanced fluorescence of oxidation products of dihydroethidium (DHE) and dichlorofluorescein diacetate. An analog of 20-HETE elicits no increase in ROS and blocks 20-HETE-evoked increments in DHE fluorescence, supporting its function as an antagonist. Endothelial cells derived from bovine aortas exhibit enhanced ROS production to 20-HETE quantitatively similar to that of BPAECs. 20-HETE-induced ROS production in BPAECs is blunted by pretreatment with polyethylene-glycolated SOD, apocynin, inhibition of Rac1, and a peptide-based inhibitor of NADPH oxidase subunit p47phox association with gp91. These data support 20-HETE-stimulated, NADPH oxidase-derived, and Rac1/2-dependent ROS production in BPAECs. 20-HETE promotes translocation of p47phox and tyrosine phosphorylation of p47phox in a time-dependent manner as well as increased activated Rac1/2, providing at least three mechanisms through which 20-HETE activates NADPH oxidase. These observations suggest that 20-HETE stimulates ROS production in BPAECs at least in part through activation of NADPH oxidase within minutes of application of the lipid.
- superoxide
- Rac1/2
- hydrogen peroxide
- reactive oxygen species
products of cytochrome P-450 (CYP) ω-hydroxylases (including CYP4 isoforms) mediate key physiological functions including autoregulation of blood flow, tubuloglomerular feedback, Na+ reabsorption in the kidney, and relaxation of pulmonary arterioles (29, 38). Our studies have focused on the role of CYP4 and its arachidonic acid (AA) metabolite, 20-hydroxyeicosatetraenoic acid (20-HETE), in pulmonary vascular function and biology. 20-HETE is the CYP ω-hydroxylation product of AA cleaved from membrane phospholipid sources. Enzymes of the CYP4A, -4B, and -4F families catalyze the ω-hydroxylation of fatty acids, and several isoforms in these families produce 20-HETE when incubated with AA. For example, rat CYP4A1, -4A2, and -4A3 catalyze AA ω- and ω-1-hydroxylations with the highest catalytic efficiency accruing to CYP4A1 (35). Although CYP4A2 and CYP4A3 exhibit an additional arachidonate 11,12-epoxidation activity, CYP4A1 operates solely as an ω-hydroxylase. Most investigators suggest that CYP4 isoforms constitute the major source of 20-HETE synthesis in extrahepatic tissues, including the lung (35, 38). Accordingly, we have investigated the effects of CYP4 product, 20-HETE, in our studies of this system in pulmonary vascular biology.
We have identified a unique role for CYP4/20-HETE in regulating pulmonary artery endothelial cell (PAEC) endothelial nitric oxide synthase (eNOS) (13, 30). Whereas CYP4 expression is widely recognized in vascular smooth muscle cells from systemic circulations, pulmonary endothelium from small arteries as well as vascular smooth muscle cells express CYP4 and convert AA into 20-HETE (45). This subcellular localization suggests distinctive biological opportunities and functions for CYP4 in pulmonary arteries. For example, possibly acting as an eNOS protein partner, CYP4 mediates VEGF-induced relaxation of small pulmonary arteries via enhanced NO release. VEGF and 20-HETE both phosphorylate eNOS and Akt, but neither enhances association of heat shock protein 90 (Hsp90) with eNOS (13, 30). Because 20- HETE mediates VEGF-induced NO release in bovine PAECs (BPAECs), one activity that these lipids might reasonably mediate is angiogenesis. Enhanced survival of PAECs is critical for recovery from lung injury, revascularization of transplanted tissues, growth and development of lungs, and others. Hence, the clinical implications of such actions, if proven, would be high.
In fact, CYP4 has been postulated to promote angiogenesis via NADPH oxidase and reactive oxygen species (ROS)-dependent mechanisms in systemic vascular beds (6, 41). Although deleterious effects of unchecked ROS are well-documented, compelling evidence now exists that ROS play a key role signaling vital physiological processes including cell growth, angiogenesis, contraction and relaxation of vascular smooth muscle, and others (7, 20). Because CYP4/20-HETE promotes angiogenesis, vascular tone, and eNOS function, we explored the potential of this system to enhance pulmonary ROS production. Our assumption is that if 20-HETE-evoked ROS is an important pathway to enhanced survival or proliferation of PAECs, we must first determine the capacity of this lipid product to modulate ROS production and the cellular mechanisms through which this effect is accomplished. We hypothesized that 20-HETE would enhance ROS production in PAECs in a manner that was associated with NADPH activation. Our data demonstrate that 1) 20-HETE increases ROS in isolated BPAECs, and 2) enhanced production is attributable, in part, to NADPH oxidase sources. 20-HETE increases membrane translocation and phosphorylation of p47phox and activation of Rac1/2 in BPAECs.
Apocynin (cat. no. 178385, Calbiochem), DPI (cat. no. D2926, Sigma), Rac assay (cat. no. BK035; Cytoskeleton, Denver, CO), 4-hydroxy-TEMPO (cat. no. 176141, Sigma-Aldrich), dihydroethidium (DHE; D7008, Sigma), dichlorofluorescein diacetate (DCFH-DA; C13293, Molecular Probes), trypsin (cat. no. 15400-054; Gibco, Carlsbad, CA), anti-CYP4 antibody (cat. no. ab622615, Abcam), polyclonal goat anti-p47 antibody (cat. no. 610354, BD Biosciences), and monoclonal anti-p47 antibody (cat. no. sc-17844, Santa Cruz). Mouse monoclonal anti-phosphotyrosine antibody (P-Tyr-100, cat. no. 9411) was obtained from Cell Signaling Technologies (Danvers, MA). Polyethylene-glycolated SOD (PEG-SOD, cat no. S-9549; 685 U/mg solid, 1 unit inhibited rate of reduction of cytochrome c by 50% in a coupled system with xanthine and xanthine oxidase at pH 7.8 at 25°C in a 3-ml reaction volume) and catalase (PEG-cat, cat. no. C-4963; 17,600 U/mg solid, 1 unit decomposed 1 μmol of H2O2/min at pH 7.0 at 25°C, whereas the H2O2 concentration falls from 10.3 to 9.2 mM) were acquired from Sigma. A chemical inhibitor of Rac1, NSC23766, was purchased from EMD Chemicals (cat. no. 553502). An enhanced chemiluminescence (ECL) kit was purchased from Amersham Biosciences, Piscataway, NJ (cat. no. 32106). A protease inhibitor cocktail was obtained from Roche, Mannheim, Germany (cat. no. 836 170). Protein determination kit (cat. no. 500-0006) and Protein Standard I (cat. no. 500-0005) were obtained from Bio-Rad.
A chimeric peptide, which inhibits association of p47phox with gp91 in NADPH oxidase, was synthesized by our protein core according to the sequence defined by Rey et al. (37) to test the contribution of NADPH oxidase to ROS production. The sequence of this peptide is [H]-R-K-K-R-R-Q-R-R-R-C-S-T-R-I-R-R-Q-L-NH2. The sequence of the scrambled (control) peptide is R-R-Q-R-R-R-C-L-R-I-T-R-Q-S-R-NH2. 20-HETE and 20-hydroxyeicosa-6,15-dienoic acid (20-6,15-HEDE, a 20-HETE antagonist) were synthesized in the laboratory of Dr. J. R. Falck (4).
Growth and culture of PAEC.
BPAEC from small pulmonary arteries (<5 mm diameter) and bovine aortic endothelial cells (BAECs) were isolated (45) and cultured in RPMI media (cat. no. 11875-093, Gibco) containing 10% fetal bovine serum (cat. no. 16000-044, Gibco) and 1% penicillin-streptomycin (cat. no. 15140-122, Gibco) in 100-mm2 dishes. Tissues from which these cells were primarily isolated were obtained from a local abattoir with all protocols reviewed and approved by the Medical College of Wisconsin. When the cells were 80% confluent, they were washed with sterile PBS (cat. no. 14190-144, Gibco). The media was changed to serum-free RPMI before experiments, unless otherwise mentioned, to remove the effects of lipids and growth factors in the serum, and 0.1% bovine serum albumin was added instead as a lipid carrier. Treatments included 10 nM to 10 μM 20-HETE, 10 nM to 1 μM antagonist 20-6,15-HEDE, 1 μM apocynin, 20 μM NSC23766, PEG-SOD (100 or 250 U) or PEG catalase (500 U), and 50 μM peptide-based inhibitor of NADPH oxidase activity.
Detection of ROS by fluorescence microscopy.
Primarily isolated BPAECs from passages 2 to 7 were used. Inhibitors of ROS were applied 30 min before loading with fluorescent dyes, DHE (final concentration 10 μM), or 2′,7′-dichlorodihydrofluorescein-DA (final concentration 5 μM). DHE is widely used as a superoxide probe since two-electron oxidation by superoxide of membrane-permeable DHE results in the impermeable fluorescent product ethidium (22). Likewise, the oxidation of the nonfluorescent 2′,7′-DCFH-DA to the intensely fluorescent 2′,7′-DCF is commonly used to detect the generation of reactive oxygen intermediates in neutrophils and macrophages, particularly hydrogen peroxide (12). However, DCF itself can also act as a photosensitizer for dichlorodihydrofluorescein-DA oxidation; in a cell-free system, dichlorodihydrofluorescein has been reported to be oxidized to DCF by peroxynitrite anion (ONOO−; Ref. 8). Therefore, we tested 20-HETE-induced fluorescence with both indicators as well as membrane-permeant superoxide dismutase and catalase to determine the contribution of superoxide and hydrogen peroxide, respectively, to the signals.
Images were acquired with a Nikon Eclipse TE200 microscope equipped with fluorescence attachment (Lambda DG-4, Sutter Instrument), captured using a Hamamatsu digital camera C4742-95, and processed using MetaMorph version 6.2 software as previously described (18, 19). Background fluorescence was estimated by capturing an image in an area free of cells and subtracted from the fluorescence intensity of cells on the same slide.
Western blot analysis.
Western blot analyses were performed according to our previously published protocols (34, 45). Protein determination was estimated using a protein assay kit (Bio-Rad; see materials and methods). Specific antibodies for Rac1/2, p47phox, and phosphotyrosine were matched with secondary antibodies and visualized with ECL Plus detection reagent (cat. no. RPN 2133, Amersham Biosciences). The blots were scanned using an Alpha Image 220 Analysis System (Alpha Innotech), and the relative densities of the bands from the same blot were compared. Each experiment was repeated at least four times using separate batches of cells to ensure reproducibility.
Cell fractionation.
BPAECs were lifted with trypsin, washed with PBS, and lysed with 25 mM Tris·HCl, 1 mM EDTA, 1 mM EGTA, and cocktail of protease inhibitors, pH 7.4, sonicated for 5–10 s on ice in VirSonic 60 and centrifuged at 18,200 g for 10 min. The pellets contain the membrane fractions, which are solubilized with RIPA buffer (50 mM Tris·HCl, 150 mM NaCl, 0.25% deoxycholic acid, 1% Nonidet P-40, 1 mM EDTA, and cocktail of protease inhibitors, pH 7.4).
Rac assays.
Rac activation assay Biochem Kit was purchased from Cytoskeleton. Two milligrams of protein from BPAEC lysates were used for each pull down. Cells were studied between 80–90% confluence in passages 4–5. In this assay, an epitope protein binds to the PAK-PBD domain (Rac effector protein, p21 activated kinase 1-p21 binding domain) in activated Rac associated with GTP. Positive controls were recombinant Rac1-His tagged protein. Proteins were separated by SDS-PAGE and identified in Western blots probed with a primary polyclonal antibody to Rac provided in the kit and used in a 1:250 dilution. Total Rac1 in cell lysates was estimated by density of Western blots obtained using the primary antibody in this kit.
Identification of phosphotyrosine in immunoprecipitated p47phox from BPAECs.
BPAECs were grown to ∼80% confluence, washed, starved for 8 h, and then treated with vehicle or 1 μM 20-HETE for times as indicated for each experiment. After treatments, cells were lysed, and 500-μg proteins were subjected to immunoprecipitation with polyclonal goat anti-p47phox (cat. no. 610354, BD Biosciences) for 16 h. Immunoprecipitates were analyzed by Western blot with mouse monoclonal anti-phosphotyrosine antibody (1:000 dilution, P-Tyr-100, cat. no. 9411; Cell Signaling Technologies) as well as p47phox using a second monoclonal anti-p47 antibody (sc-17844). The relative densities of phospho-p47phox bands were compared in scanned and quantitated images of the blots using the Alpha Image 220 Analysis System detailed above.
Statistical analysis.
For all DHE or DCF fluorescence studies, a minimum of 60 cells for each test condition and two separate isolations of cells were used. For all Western blots, a minimum of four studies from separate cell isolates were performed. Pooled data from each experiment were used to calculate the means ± SE for control (vehicle treated) or experimental (treated with 20-HETE, inhibitors, or inhibitors + 20-HETE) samples. The data were tested for significance by a Student's t-test (for 2-paired or unpaired samples) or Mann-Whitney rank sum test for three or more groups using the Jandel SigmaStat software. Experiments with P < 0.05 were considered significant.
20-HETE increases superoxide in primary cultures of BPAECs in a concentration- and time-dependent manner.
BPAECs (passage ≤7) were loaded with DHE (10 μM) and imaged with fluorescence microscopy (Fig. 1, A and B). In these experiments, 20-HETE (from 10 nM to 10 μM) or vehicle was added to the bath after baseline values were obtained, and then images were captured after 5 min. Vehicle (ethanol) alone did not change DHE fluorescence. 20-HETE at all concentrations >10 nM significantly increased fluorescence (Fig. 1A). The fluorescence response peaked at 1 μM 20-HETE and decreased at higher concentrations.
Next, we tested time-dependent responses to a fixed concentration of 20-HETE, which gave maximal increments in DHE fluorescence (Fig. 1B). In these experiments, cells were preincubated with DHE for 20 min and washed, and then 1 μM 20-HETE was added. Images were acquired at the times indicated on the x-axis of Fig. 1B after addition of 20-HETE. Vehicle (ethanol alone) had no effect on DHE fluorescence. Similarly, values obtained within the first 15 s after the addition of 20-HETE were not different from those of vehicle alone (t = 0; see Fig. 1B). The peak intensity in DHE fluorescence was elicited within 5 min of application of the lipid (Fig. 1B). Thereafter, increased DHE fluorescence was noted for at least 60 min in cells treated with 20-HETE relative to that of cells treated with vehicle. For further experiments detailed below, we evaluated the effect of 1 μM 20-HETE on DHE fluorescence 5 min after application to the bath unless otherwise noted in the text.
To examine the dependence of increase in DHE fluorescence in BPAECs treated with 20-HETE on superoxide, we tested the capacity of PEG-SOD, a cell-permeable superoxide dismutase, to blunt DHE increases in cells treated with 20-HETE (24). In cells pretreated with 100 or 250 U PEG-SOD, the addition of 20-HETE to the bathing solution evoked no increase in the DHE signal (Fig. 2). These data support a contribution of superoxide to 20-HETE-evoked DHE fluorescence signals.
20-HETE increases H2O2 in BPAECs.
Because superoxide is rapidly dismuted in vivo to hydrogen peroxide, we tested the capacity of 20-HETE to increase H2O2 in BPAECs. 2′,7′-Dichlorodihydrofluorescein-DA is a fluoroprobe that, after deesterification by intracellular esterases, undergoes oxidation to a fluorescent compound, DCF. DCF fluorescence in BPAECs preincubated with 5 μM probe was increased by the addition of 20-HETE, but not vehicle, to the bath (Fig. 3). To help exclude potential nonspecific fluorescence of DCF, we tested the potential of PEG catalase (21) to blunt the 20-HETE-induced DCF signal in these cells. We observed effective inhibition of 20-HETE-induced increase in fluorescence in cells treated with 500 U PEG catalase.
20-HETE-evoked ROS production is not mimicked by structurally similar lipids.
The specificity of 20-HETE in producing ROS in BPAECs was tested by use of a structurally similar lipid, 20-6,15-HEDE, which, compared with 20-HETE, is missing two double bonds (between carbons 8-9 and 11-12) and has the NH2 and COOH terminus CO2 H and OH groups switched (Fig. 4). This compound does not cause constriction of renal or cerebral arteries as does 20-HETE but is reported to function as an antagonist to 20-HETE in renal arteries (4). As opposed to 20-HETE, this compound did not evoke increases in ROS production over that of vehicle control (Fig. 4) with 10 nM 20-6,15-HEDE decreasing ROS production over that of vehicle alone. Moreover, pretreatment with this analog significantly blunted 20-HETE-evoked increases in ROS, the most effective concentration in this regard being 100 nM. These data support the position that 20-HETE-induced change in ROS production in BPAECs does not represent a bulk or nonspecific lipid effect and that this analog acts as an antagonist in BPAECs as well as renal arteries.
Vascular bed-independent effects of 20-HETE on ROS production.
To test the effect of 20-HETE on ROS production in endothelial cells from a different vascular bed, we examined the capacity of this lipid to change DHE fluorescence in BAECs, which do not express CYP4A (45). BAECs treated with 1 μM 20-HETE exhibited an ∼2-fold increase in DHE fluorescence above baseline (Fig. 5), quantitatively similar to the response of BPAECs albeit with a peak at 10 rather than 5 min. These data demonstrate that endothelial cells from systemic as well as pulmonary vascular beds respond to 20-HETE with increased ROS production. They also suggest that endogenously generated 20-HETE is not essential for the effect of exogenous 20-HETE to increase acutely intracellular superoxide.
NADPH oxidase inhibitors block 20-HETE-evoked increases in ROS.
To investigate the contribution of NADPH oxidase to 20-HETE-evoked ROS production, we pretreated BPAECs with 1 μM apocynin (32), a chemical inhibitor of NADPH oxidase, for 30 min. This treatment largely blocked 20-HETE-induced increases in ROS production as detected by DHE (Fig. 6). These data suggest that 20-HETE may enhance ROS in these cells at least in part through activation of NADPH oxidase.
We also tested a second, peptide-based inhibitor of the association of p47phox to gp91 (Fig. 7; Ref. 37). Treatment with this peptide effectively blocked 20-HETE-evoked ROS production, consistent with a NADPH oxidase contribution to this endpoint. A scrambled peptide also partially blocked 20-HETE-evoked increases in ROS, suggesting that some component of the peptide-associated protection is nonspecific.
Rac1/2 contributes to 20-HETE-induced ROS production.
Test groups of BPAECs were pretreated for 30 min with 20 μM Rac1 inhibitor, which impairs ability of Rac1 to interact with intracellular effectors (NSC23766; Ref. 17), or vehicle and then loaded with DHE and imaged after treatment with 20-HETE or vehicle for this lipid. Cells treated with the Rac1 inhibitor exhibited no increase in ROS production as tracked by DHE, whereas those treated with vehicle for this compound did (Fig. 8). These data support a role for Rac1 signaling in 20-HETE-induced ROS production of BPAECs.
Treatment with 20-HETE translocates and phosphorylates p47phox.
Pulmonary vascular endothelia express at least two forms of NADPH oxidase, Nox2 and Nox4. Nox2 is activated by the recruitment of cytosolic component p47phox to the membrane (25, 33) and phosphorylation of serine (23) and tyrosine (16) residues. To further examine the mechanism of 20-HETE-induced activation of NADPH oxidase in BPAECs, we studied the effect of 1 μM 20-HETE on the translocation and phosphorylation of p47phox-associated tyrosine. Treatment with 20-HETE increased membrane-associated p47phox 10 min after application of the lipid over that of vehicle (ethanol) alone (Fig. 9).
Next, we immunoprecipitated BPAEC lysates with a polyclonal antibody to p47phox and then probed for phosphorylated tyrosine (as well as p47phox using a monoclonal antibody for an internal control). 20-HETE increased tyrosine phosphorylation of immunoprecipitated p47phox in a manner that peaked ∼10 min after exposure to 20-HETE (Fig. 10).
Rac1/2 activation increases with 20-HETE.
We examined the effect of 20-HETE on GTP-bound Rac1/2 in BPAECs with the hypothesis that Rac1/2 activation may promote 20-HETE-induced ROS production. 20-HETE increased the density of GTP-bound Rac in BPAECs. This response peaked between 10–20 min after exposure to the lipid (Fig. 11). These observations are consistent with reports by Ushio-Fukai et al. (39, 40) and Bedard and Krause (7) that associate activation of NADPH oxidase with activation and recruitment of Rac1/2 to the membrane in vascular cells.
Our studies confirm, for the first time, acute 20-HETE induced time- and concentration-dependent enhanced production of superoxide in BPAECs. This conclusion is supported by 20-HETE-induced signals of DHE- and DCF-derived fluorescent product(s), which peaked within 5 min of application of the lipid, and blunting of the signal by pretreatment of the cells with PEG-SOD and PEG catalase, respectively. DHE signal increased in cells treated with 20-HETE but not vehicle or a structurally similar analog that acts as an antagonist in renal or cerebral vessels (4, 38). The signal detected by DHE should represent superoxide (44), but other metabolites are almost surely generated as well. Moreover, hydrogen peroxide rather than superoxide may be the effector ROS product stimulated by 20-HETE. Because we did not explore further the chemical nature of 20-HETE-induced ROS products, we refer in this work to the products promoted by 20-HETE simply as ROS.
Regulation of NADPH oxidase in renal arteries by CYP4 was recently reported by Wang et al. (41). In that study, overexpression of CYP4A using adenoviral vectors carrying the CYP4A construct (administered via intravenous administration) in Sprague-Dawley rats increased synthesis of 20-HETE in renal interlobar arteries as determined by gas chromatography-mass spectrometry (GC/MS) analysis. Rats overexpressing CYP4A demonstrate increased generation of superoxide and increased expression of gp91 (41). The contribution of endothelium to ROS production, or the acute effect of 20-HETE itself (as opposed to CYP4 overexpression), was not examined. We are particularly interested in the influence of 20-HETE on pulmonary artery endothelial cell ROS production because CYP4 is uniquely expressed in these cells, whereas it has not been reported to be so in endothelium from other vascular beds. Our studies are the first to report enhanced ROS production in BPAECs treated with 20-HETE.
This work also shows that enhanced production of ROS by 20-HETE is not a nonspecific effect of lipids and that 20-HETE stimulates ROS in aortic endothelial cells from the same species in a similar magnitude as endothelium from small pulmonary arteries. CYP4A protein is not expressed in BAECs, but vascular smooth muscle cells from many systemic, as well as pulmonary, circulations express CYP isoforms that are capable of metabolizing AA to 20-HETE (38). Moreover, our data imply that BAECs as well as BPAECs have the cell signaling mechanisms to increase ROS in response to 20-HETE. Therefore, it is possible that smooth muscle-derived 20-HETE acts as a paracrine factor, stimulating ROS release in subjacent endothelial cells. There is precedent for paracrine actions of endothelial and smooth muscle-derived factors: NO decreases the state of activation of cerebral and renal arteries through inactivation with covalent interaction of the prosthetic heme group to CYP4A enzymes (e.g., Ref. 5).
We also performed some experiments to examine the source of 20-HETE-evoked ROS production in BPAECs. Although ROS may be produced from several cellular sources, including mitochondrial electron transport chain, xanthine oxidase, CYP, uncoupled NOS, and NADPH oxidases (36), the dominant sources in endothelium are believed to be mitochondrial electron transport and NADPH oxidase (36). Indeed, there is excellent evidence of "cross talk" between mitochondrial and NADPH oxidase sources of ROS in pulmonary artery endothelial cells, suggesting that there may not be a simple or single contributor to enhanced ROS production in any cell or tissue type. For example, hyperoxia induces ROS from mitochondrial sources in pulmonary capillary endothelial cells but subsequently activates NADPH oxidase through calcium-dependent and Rac1-dependent mechanisms (9). Our data demonstrate that inhibition of NADPH oxidase by two mechanistically distinct agents (1 peptide-based inhibitor and 1 chemical) significantly blunted 20-HETE-evoked increases in ROS in BPAECs. There is evidence that NADPH oxidase may be a particularly important form of ROS in BPAECs. In support of this view, Al-Medhi et al. (2) have identified endothelial NADPH oxidase as a primary source of ROS in ischemic lungs. Gupte and colleagues (27) have reported that bovine pulmonary arteries generate 60–80% more superoxide than similarly sized bovine coronary arteries, and >50% of this signal was abolished by the NADPH oxidase inhibitor, apocynin, in pulmonary arteries.
Guo et al. (26) recently reported enhanced DHE fluorescence in human umbilical vein and dermal microvascular endothelial cells treated with 20-HETE and an analog of this compound, WIT003. Our results stand in contrast to those of these investigators (26) in the dependence of 20-HETE-stimulated ROS on NADPH oxidase in endothelial cells. Most of the experiments of Guo et al. (26) were performed with 20-HETE analog, WIT003, at concentrations higher than those of 20-HETE we used, so we cannot exclude differences in signaling pathways based on these factors. Also important may be the different origin of endothelial cells investigated in the two studies. Human dermal microvascular endothelial cells may well express different Nox isoforms and/or activate signaling pathways distinct from those of pulmonary artery endothelial cells. Finally, the experimental approach employed by Guo et al. (26) was different from ours, focusing on 20-HETE-mediated signaling on VEGF-induced ROS production and signaling in endothelial cells rather than effect of 20-HETE on components of the NADPH oxidase as we did.
To obtain further insight, we investigated mechanisms through which 20-HETE may activate NADPH oxidase. In its activated form, NAPDH oxidase is a multimeric protein consisting of at least three cytosolic subunits, including p47phox, p67phox, p40phox, either Rac1 or Rac2, and a membrane-associated cytochrome reductase complex consisting of gp91 and p22phox (e.g., Refs. 16, 33). Activation of vascular NADPH oxidase (Nox1 and Nox2) is characterized by recruitment of p47phox from cytosolic to membrane pools and phosphorylation of at least three amino acid residues (33). Phosphorylation of p47phox from human neutrophils occurs at multiple serine residues (23). However, Src-mediated tyrosine phosphorylation of p47phox is associated with activation of NADPH oxidase and generation of ROS in human PAECs (16). Results of the present studies demonstrate that 20-HETE promotes translocation of p47phox to the membrane and enhances tyrosine phosphorylation of p47phox in BPAECs. Moreover, activation of Nox1 and Nox2 is promoted by binding of Rac1 to Noxa1 or other regulatory subunits (e.g., Refs. 7, 15, 39). Our data show that 20-HETE increases GTP-bound Rac1/2 in BPAECs, another feature characteristic of NADPH oxidase activation. Activation of Rac1 is necessary for 20-HETE-induced ROS production in our BPAECs in that inhibition of this pathway blocks HETE-evoked fluorescence. Thus, in addition to blunted 20-HETE-evoked ROS production in BPAECs by NADPH oxidase and Rac1/2 inhibition, we show for the first time that, in BPAECs, 20-HETE acts on three endpoints in the signaling pathway of NADPH activation: translocation and tyrosine phosphorylation of p47phox and activation of Rac1/2. Based on the sum of these observations, we speculate that one mechanism through which 20-HETE increases ROS production in BPAECs is through activation of NADPH oxidase. It is interesting that the peak in 20-HETE-evoked DHE fluorescence appears within ∼5 min, whereas phosphorylation and Rac activation in stimulated cells peaks 5–10 min later. It is possible that crude temporal resolution accounts for this difference in peak responses. Also, some mechanisms of NADPH oxidase activation other than the endpoints examined (and there are many) or cross talk between mitochondrial and NADPH oxidase-stimulated ROS production may be responsible for this difference (3). If so, 20-HETE may be as important in sustaining or amplifying stimulated ROS production as initiating it.
Whereas both Nox2 and Nox4 are expressed in pulmonary artery endothelial cells, only Nox2 is activated by binding of p47phox (31). Therefore, given 20-HETE-induced translocation and tyrosine phosphorylation of p47phox and Rac1/2 activation in BPAECs and apocynin- and peptide-based inhibition of NADPH oxidase on evoked ROS production, we believe that 20-HETE affects at least Nox2 isoforms in these cells.
What might be the implications of stimulated ROS production in BPAECs? ROS including superoxide and hydrogen peroxide signal a host of processes in endothelial cells including stimulation of growth and migration and activation of transcription factors and protein kinases including ERK, p38 MAPK, and Akt (20, 28). Uncontrolled release of particularly mitochondrial-derived ROS has been associated with pathologies such as diabetes and Alzheimer's. Impaired pulmonary arterial endothelial dysfunction (blunted acetylcholine-induced relaxation) in mice exposed to hyperoxia depends on gp91phox, which appears to trigger rather than directly mediate impaired NO-dependent relaxation (24). Thus some actions of increased ROS production and activation of NADPH oxidase in pulmonary vascular cells promote dysfunction.
However, these oxygen products are also essential to physiological signaling in vascular cells (e.g., Refs. 36, 42). Hydrogen peroxide functions as a dilator of pulmonary arteries (10, 11). Furthermore, hydrogen peroxide in nanomolar-to-micromolar concentrations stimulates proliferation and migration of BAECs (43). Human coronary and dermal microvascular endothelial cells require ROS derived from NADPH oxidase for proliferation and migration (1). Moreover, hydrogen peroxide transactivates mitochondrially derived growth factors and downstream signaling in human umbilical vein endothelial cells, further supporting a role for ROS in mediating endothelial cell growth and proliferation (14). Angiogenesis induced by VEGF-suffused subcutaneously implanted sponges is deficient in Nox2 knockout mice, implicating both NADPH oxidase and ROS in this process (40). Although the implications of our observations remain to be determined, the above detailed roles of ROS in vascular cells raise the possibility that CYP4/20-HETE-stimulated ROS in BPAECs may serve to promote vascular repair after acute lung injury, severe pneumonia, or other conditions of injury as well as modulate pulmonary vascular tone.
Financial support was provided by National Institutes of Health Grants HL-49294 (E. R. Jacobs), HL-68627 (E. R. Jacobs), HL-069996 (M. Medhora), and GM-31278 (J. R. Falck) and the Robert A. Welch Foundation (J. R. Falck).
We gratefully acknowledge the participation and input from investigators in the Free Radical Research Center and the Cardiovascular Center at the Medical College of Wisconsin. We also thank Laurel Dunn for help in processing the manuscript.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2008 the American Physiological Society


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Anagrammer is a game resource site that has been extremely popular with players of popular games like Scrabble, Lexulous, WordFeud, Letterpress, Ruzzle, Hangman and so forth. We maintain regularly updated dictionaries of almost every game out there. To be successful in these board games you must learn as many valid words as possible, but in order to take your game to the next level you also need to improve your anagramming skills, spelling, counting and probability analysis. Make sure to bookmark every unscrambler we provide on this site. Explore deeper into our site and you will find many educational tools, flash cards and so much more that will make you a much better player. This page covers all aspects of EPIGONI, do not miss the additional links under "More about: EPIGONI"


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Air flow through a building can have a powerful impact on comfort, expense, and air quality. Blower doors provide a way to quantify air flow and the resulting heat loss, along with a way to pinpoint specific leaks.
A blower door is a device for testing the airtightness of a building. It consists of a calibrated fan for measuring an air flow rate and a pressure sensing device to measure the pressure created by the fan flow. The combination of pressure and flow can be used to estimate the airtightness. The airtightness of a building is useful knowledge when trying to increase energy conservation or decrease indoor air pollution.
In many buildings, the most cost-effective and easiest measure toward increasing energy efficiency is sealing the building envelope. It is vitally important, therefore, to determine the extent to which your home or buiding is leaking air and locate all areas of leakage.
Many customers will say they already know where the cold air is entering their house, so a blower door test is not necessary. While this may be true, two factors must be acknowledged. First, knowing where cold air enters your house is only half the problem. Any amount of air entering the house is accompanied by air leaving the home somewhere else. Those leaks are harder to locate. By pressurizing the entire building relative to the outdoors, we can locate all the air leaks. Second, a certain amount of ventilation is needed in a house to prevent the buildup of moisture, pollutants, and earth and combustion gases, which can be deadly! A blower door test allows us to quantify the leakiness of the building and become aware of potential hazards.
Blower door testing is included for no extra charge for our customers in our energy audit package.
E-mail: firstname.lastname@example.com
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Matrix Antennas specializes in home theatre installation in the Albury Wodonga area and surrounding district. We have years of experience at creating the perfect system and solution for your house and needs. From installing a surround sound system in a dedicated home theatre room through to multi room sound systems we can advise, install and get you the best result for your budget.
Whether it is from a simple wall mount TV, multi room audio or the Full Home Theatre experience, we can provide expert installation at an affordable price. Both new-construction and retrofit installations are offered, and Matrix Antennas takes responsibility for adhering to all important safety considerations, standards, and codes.
Wanting existing equipment installed?
If you have simply purchased some home entertainment components and wish to have the system installed and set up to the best of its ability, we can provide expert integration and will thoroughly demonstrate your new system upon completion of home theatre installation.


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Conduct Advisory, Rates
- In return, we offer a competitive salary plus 25% cash and benefit funding programme
- London, England, United Kingdom
- Permanent, Full time
- 14 Oct 17 2017-10-14
This is an excellent opportunity to provide Conduct Advisory coverage of the Flow Rates trading and sales business within NatWest Markets
The BusinessWe're looking for a Conduct Advisory professional to join us in London
- This is an excellent opportunity to provide Conduct Advisory coverage of the Flow Rates trading and sales business within NatWest Markets (NWM)
- You'll play a leading role in ensuring that the team delivers a high standard of compliance advice and support across all businesses covered
- We'll look to you to develop a strong understanding of the business, and to proactively guide and lead the business on conduct and compliance matters
- We believe that flexible working can make a real difference to your wellbeing and professional development, which is why we offer a range of flexible working arrangements
What you'll do
This role will see you joining the Currencies and Flow Rates team within our Conduct Advisory function, responsible for providing compliance coverage to the trading and investment banking businesses of NWM. Your primary focus will be on the Flow Rates business but you'll also collaborate with the wider team on a variety of activities.
You'll be responsible for providing compliance support to the Flow Rates business. This will involve advising trading and sales staff, and senior management, on regulatory compliance and conduct standards such as those that relate to the treatment of clients, and to behaviour in financial markets.
The skills you'll need
To be successful in this role, it's essential that you have fixed income advisory compliance experience.
You'll also need:
- Strong oral and written communication skills
- The ability to convey complex issues and concepts in a clear and concise manner
- Strong people skills, and the ability to work collaboratively in teams and on complex programmes
- Influencing and conflict resolution skills
- A keen interest in financial markets and conduct matters, with a strong desire to learn more
- To be a self-starter
How we'll reward you
In return, we offer a competitive salary plus 25% cash and benefit funding programme that can be tailored to suit your individual needs. In addition, we provide a wide selection of exclusive lifestyle offers, development and learning programmes, services and support designed to help you manage and balance your work/life priorities.
Visit our http://jobs.rbs.com/pages/reward-and-benefits reward and benefits page for more information on the benefit packages we offer.
At RBS, we want everyone to feel welcome, regardless of your background or needs. If you need adjustments making to your working environment, we'll do everything we can to support you. As part of this commitment, we offer flexible working options for some of our roles - http://jobs.rbs.com/pages/flexible-working find out more.
As a Financial Services organisation we comply with and support the requirements set by our Regulator, the Financial Conduct Authority (FCA), which are designed to protect our customers. This role falls under Conduct Rules of the Individual Accountability Regime (IAR) and is subject to pre-employment screening. This means if your application is successful, you'll need to satisfy some important background checks before you can start working with us. These will include a full credit check, a criminal record check, residency and right to work checks.


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I mean no disrespect to all the people who served in Iraq and Afghanistan. Their courage and dedication is not in question.
But the absolute uselessness of those extended conflicts reduced them to cannon fodder.
You disrespect all of America, all of the veterans, and all of the military when you label us cannon fodder, and say that we use our GIs as cannon fodder and fight as cannon fodder.


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Stacia is a peasant-style tiered dress. With lots of swirl.
The dress has a slightly lowered neckline with a casing for elastic. The garment pulls over the head and has short or long sleeves finished with elastic casings. The top two of three tiers have a contrasting band on the bottom that overhangs the next tier. The underarms of yoke feature narrow ties to nip in the waist for a slim look.
Available in sizes 1-10 all in one package.
Difficulty: ** Moderate
If you have any questions, please just let me know.
More fabrics, laces and patterns are available on my web site www.sweet-stitches.com
I ship within 1-2 business days using USPS 1st class shipping whenever possible.
When I find that the shipping charged was higher than necessary I will refund the overage.
Damaged items can always be returned.
Regrettably cut fabric under 4 yards can not be returned because it is difficult to resell once removed from the bolt.
When items are returned the postage will be paid by the buyer. Refund will be for the cost of the item only.


text: I think it is very luxurious to have empty beaches on your holidays, but there where not many people on the wonderful beaches. The season has ended really, but the weather is still nice and the ocean is warm. It was wonderful to have the place without a lot of tourists all over the place. It was a calmness all over our destination and the beaches where just one of many things. I loved that country and the people living there had no stress. The food was good and everyone was friendly. I liked talking to the people I meet. It was nice. I meet nice people. The nature was grand and different. Tormented by the warmth and lack of water, gives a different character to the landscape and promote a different kind of plant life than at home.


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spiral classifier with satisfactory service
(An English-Chinese-Japanese Dictionary of Technology,606812,S/74892) MSC, ==> Main Ship Equipments . Equipment . 2016 ACC Program . Wednesday July 6, 2016 10:10-10:30, Paper WeA11.3 Add to
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Today's Mass Readings Saturday 18th Week of the Year Matthew 17:14-20 Have any of us prayed for someone or something and are still waiting for the Lord to answer that prayer? Perhaps something comes to mind right now. The disciples had this experience in today's gospel. After kneeling before Jesus and explaining to him […]Read more
Tag: mustard seed
Monday 17th Week of the Year
Today's Mass Readings Monday 17th Week of the Year Matthew 13:31-35 Today's Gospel reminds us that God's kingdom grows, and Jesus says that the kingdom may start seemingly small in the seeds of faith planted in our hearts. Let us place all our faith in the One who planted those seeds. I remember lying in […]Read more


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Shootrium game
A simple shooting game that requires you to avoid and shoot monsters and survive as long as possible! Deploy turrets to aid you in survival and get power ups to increase stats.
Shootrium, play free Shooting games, related games and updates online. Added on
Author: mgf 11358
Here are some more related games in Shooting category. Also you can jump to other category using box above.
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Related games
- Box Dude Tower Defence 4
- Zombo Buster
- Rebel Fortress Survival
- Abe Clone Wars
- That Girl With a Gun
- Pirates Vs Prince
- Holiday Survival Halloween
- KILL BILL iard-2
- Dekay Hunter
- Fursa Hunter
- Bender Hunter
- Shelly Hunter
- Fer Hunter
- Zoombie Factory
- The Italian Mafia
- Viking War by flashgamesfan com
- Military Combat 3D
- Madville
- Super Shark Shooter
- Aliens Shooting
- Counter Shooter 2
- Zombie Runner
- Tek Tactical
- Turn Based Marine War
- Hugo with AK47
- Balloon Blast
- Dead Paradise 2
- Zombie Rescue Squad


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Poly Map
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Join Date: Feb 2010
Location: Canada
Posts: 2,435
Originally Posted by
Ok, as you want to modify the chart. When I first saw it, I naturally searched for the spot I would feel most comfortable being located. And there was no cross-mark. It should be in the Poly/Mono Relationships overlapping with Polyamorous Relationships. I am not that great with texts, but something like:
"I don't care how many my wife loves as long as she still loves me. Btw, did I introduce her boyfriend to you? Great guy!"
Edit: Only for the positive side of things obviously
one could portrait the common strugle as well.
Yep, this is pretty much my issue with it. All of the "polyamorous relationships" block overlaps with something else... And that wouldn't be a problem except for the fact that the entry that's only in "open relationship"'s refers to "it's just sex" so I don't see where my relationship style fits in there. Perhaps that "open relationships" box is meant to include romance, but then why not put the "we can have sex with other people as long as there's no love" into the swinging section.
I assume it intends to label me [[see sig for my opinion on labels]] as polyfidelitous, only I'm not. Because, as I understand it, that refers to closed triads etc.
And then there's all the confusing spots. Like... "I'm a pro domme" being inside the "polyamorous relationships" box. Last time I checked, hiring a professional anything automatically makes it not a romantic relationship. I've actually got a couple friends who are pro dommes, and they have very strict policies about no contact with their clients outside of their sessions/negotiations.
And so on.
Basically, it's an amusing cartoon, it might help clarify things for some people, but I think it's much more likely to muddle things up for most people.
As I am sure any cat owner will be able to tell you,
someone else putting you in a box is
entirely different
from getting into a box yourself.
View Public Profile
Find all posts by SchrodingersCat


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Panorama Diamond Villa in Voula with Luxury Interior Design
Panorama Diamond Villa is located in Voula, in the southern suburbs of Athens. This villa has luxury interior design and has beautiful view of sea and mountains. Through large windows beautiful nature view can be enjoyed from interior room. This luxurious family home features five bedrooms and seven bathrooms. Some of the amenities of this place include a four car garage, two fireplaces, a heated swimming pool with natural pebble finishes, an outdoor Jacuzzi. Moreover, the villa has in-floor heating, built-in ceiling speakers in most of the rooms, custom lighting and many more. Really luxury villa.
Reference : - http://www.panoramavoulas.com/
- http://freshome.com/2011/07/22/guarding-mountain-and-sea-vistas-in-athens-panorama-diamond-villa/


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by Dr. Avigdor Bonchek
Back to This Week's Parsha | Previous Issues
Parashas HACHODESH (64)
This week in addition to the weekly sedras of Vayakhel-Pikudei we also read Parashas Ha Chodesh, the portion of the new moon, from parashas Bo, Exodus Chapter 12. This is the last of the four Parshios that precede Pesach every year. HaChodesh always comes on the Shabbat before Rosh Chodesh Nisan. Following is the first and main verse of this section and the Rashi-comment on it.


text: Tom Cook has been developing applications for chemistry, government, flight simulators, education and other industries for over 18 years. This has led to his curiosity about how to decrease code complexity and time-to-market while increasing customer satisfaction. He is currently curious about DevOps and its ability to accomplish these goals.


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Froth flotation is one of the processes used to recover recycled paper. In the paper industry this step is called deinking or just flotation. The target is to release
I just want to remind everyone about Doc at He is a stand up guy! Reputable Harley tuners around here are rare so I set about
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ALSO READBASF to build centre for flooring and waterproofing systems in Germany Infosys BPO to transform financial, accounting processes for AkzoNobel AkzoNobel retains number one position on Dow Jones Sustainability Indices AkzoNobel to streamline business in Germany AkzoNobel plans to phase out organic peroxides production at Deventer by 2016
AkzoNobel will invest more than Euro 50 million in China to build new manufacturing facilities for its Powder Coatings and Decorative Paints businesses, adding further momentum to the company's strategic growth ambitions. Both new facilities will be located in Chengdu with the powder facility scheduled to open in 2014 and the deco site starting production in 2015.
The announcement follows the recent start-up of commercial production of Bermocoll cellulose derivatives at the company's multi-site in Ningbo, which will serve the growing demand from customers throughout the region, as well as from AkzoNobel's own decorative paints business. Cellulose derivatives are used in water-based paints and building additives applications.
ALSO READ: AkzoNobel plans to phase out organic peroxides production at Deventer by 2016
Ton Buchner, CEO, AkzoNobel, said, "China is playing an increasingly important role in our growth strategy and by expanding production capacity we will further strengthen our position as a leading supplier to the Chinese market. We already have a strong manufacturing footprint in eastern, central and southern China."
He added. "As demand and volumes continue to rise, we will extend our production and distribution westwards and the new sites will enable us to better serve our customers whenever and wherever they need our products."
ALSO READ: ALTANA acquires specialty coatings business from Henkel
Chengdu is one of the fastest growing cities in south western China and has consistently delivered double digit growth over the last two decades. It has established itself as an important industrial hub, resulting in high demand for quality powder coatings and decorative paint, which is expected to remain strong as the area continues to develop.


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LITITZ, PA. — Cargill will make certification options available to its North American customers for Cargill's Gerkens cocoa powders as well as its Peter's, Wilbur and Veliche chocolate brands. The certification options are UTZ Certified, Rainforest Alliance and Fairtrade.
"It means manufacturers will be able to offer products to their consumers with greater reassurance they have been produced sustainably and that they are making a positive difference to smallholder cocoa farms," said Bryan Wurscher, president of Cargill's cocoa and chocolate business in North America.
UTZ Certified stands for sustainable farming and better opportunities for farmers and their families. The UTZ program is designed to enable farmers to learn better farming methods, improve working conditions and take better care of their children and the environment.
Through its mission, the Rainforest Alliance works to conserve biodiversity and ensure sustainable livelihoods by transforming land-use practices, business practices and consumer behavior.
Products that bear the Fairtrade logo come from farmers and workers who are justly compensated. Fairtrade helps farmers in developing countries build sustainable businesses that positively influence their communities.
Cargill is training more than 60,000 cocoa farmers in the Ivory Coast and 15,000 more in Ghana. The company is on target to source more than 100,000 tons of certified sustainable cocoa beans from the Ivory Coast by 2015. Cargill is active in other cocoa producing markets, including Cameroon, Brazil, Vietnam and Indonesia.
1The latest packaging innovations from meat snack brands
2B.B.U. awarded $2.1 million in trade secret dispute
3Hostess launches Molten Lava Ding Dongs
4Grupo Bimbo finalizes acquisition of East Balt
5Six pressing issues discussed at AACCI's annual meeting
( more )
1Hostess launches Molten Lava Ding Dongs
3The latest packaging innovations from meat snack brands
4Hearthside-VSI partnership ignites bar innovation
5Grupo Bimbo finalizes acquisition of East Balt
1Contract manufacturing FAQ and tips
2Weston Foods to locate U.S. headquarters in Indiana
to the PRINT edition, or click below for free INSTANT ACCESS to valuable news and insights in the Digital Edition.


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The House
Our lovely house Grade II listed house dates from the 16th Century. Tucked away in picturesque historic Stratton, it has been lovingly and stylishly restored to create a stunning guest house. We have been rated by Visit England as 4 Star Gold Award, recognising our provision of high quality yet affordable accommodation.
Facilities include:
- Large attractive terraced gardens with plenty of space for guests to relax and enjoy a drink from our bar
- Free on-site parking with ample room for all guests
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- Fully licensed bar
- Spacious Guest Lounge with comfortable chairs and a seasonal open log fire
- On-site spa treatments available in our own treatment room with our fully qualified therapist
for guaranteed best rates


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Lewis Hamilton was awarded two penalty points for his incident with Daniel Ricciardo during the Hungarian Grand Prix.
The Mercedes driver made contact with Ricciardo at the restart, when the Australian attacked around the outside at Turn 1.
Hamilton went wide and damaged Ricciardo's car, before being handed a drive-trough penalty for the collision.
Post-race, he was awarded two penalty points, his first over the past 12 months.
Daniil Kvyat, who finished second, was also awarded two penalty points for gaining a position by going off the track. It was also his first penalty points.
Lotus driver Pastor Maldonado accumulates six penalty points after being awarded two for causing a collision with Force India's Sergio Perez.
"They were very strict on driving," said Maldonado, who was given three penalties today.
"It was different to the past, especially from the start of the season when it was more open to fighting."
Teammate Romain Grosjean was also given two penalty points - for a total of six - for his unsafe release during the race.
Finally, Max Verstappen was awarded three penalty points - for a total of five over the course of the season - after he failed to stay above the required time set by the FIA ECU during the deployment of the safety car.


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Love Yoga - or want to try it? A monthly class by Yoga I.S. benefits the animals at the Kentucky Humane Society.
Dinner tickets to the Kentucky Humane Society's Tuxes & Tails Gala are sold out. But tickets to our Emerald City Lounge are available and going fast!
Tickets are going fast for our annual Tuxes & Tails Gala! New this year are Emerald City Lounge tickets for late-night revelers.
Join world-renowned magicians and Louisvillians Lance Burton and Mac King at the Louisville premiere of the award-winning film, "Billy Topit Master Magician," at The Kentucky Center's Whitney Hall on Tuesday, July 11. There will be a red carpet with the stars and adoptable pets at 6 p.m., followed by the 7 p.m. movie screening.
The Kentucky Humane Society's Steedly location is closed, May 6 for Derby. Our Healthy Pets Clinic and Admissions offices are closed. Our Lyndon Campus is closed as well. We are open Friday during Oaks. You may visit any of our nine Louisville area Feeders Supply adoption locations on Saturday to view animals and to adopt.
Have a safe and happy Derby.
Do you love kittens? Attend our Kitten Shower and meet some adorable kittens Sunday, March 12, 1-3 p.m. at East Campus, 1000 Lyndon Lane!
Waggin' Trail Festival for the Animals will be held June 11, from 2 – 6 p.m. at the Louisville Water Tower. We want everyone to join us for this great fundraiser. Register by March 18 to get $5 off the registration fee. That means that until March 11, adult registration is $30, and child registration is $20.
Interested in making a donation to the Kentucky Humane Society before Dec. 31? Here's how:
Picture Your Pets with Santa - held at all 17 Feeders Supply stores Dec. 3-4 and 10-11 - raised over $100,000 for five local animal charities: the Kentucky Humane Society, Floyd County (Ind.) Animal Rescue League, Shamrock Pet Foundation, Saving Sunny Inc., and the Franklin County (KY) Humane Society.
Louisville radio stations are running ads against a national humane society. We want to assure you that KHS is a local nonprofit and is not a chapter of any national humane group.
A second donor has offered to match every donation to the Kentucky Humane Society before Dec. 31, 2016.
The Kentucky Humane Society's annual fundraiser Picture Your Pets with Santa will arrive with the holiday season, and we need your help. We are looking for Santas and other volunteers to make this event happen.
A donor has offered to match every donation to the Kentucky Humane Society that is received before Dec. 31, 2016.
The Tim Faulkner Art Gallery is inviting artists of all media to submit artwork for "Paws over Portland," an animal-themed art show benefiting the dogs, cats and horses at the Kentucky Humane Society (KHS).


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Top Ten Tuesday: Ten Things To Do When You Have Bloggers Block
Read Other Blogs: Sometimes reading the thoughts of what others deem as funny
or important can spark ideas in yourself.
Talk To A Friend: Bounce an idea off of someone close to you. I usually think
of a number of blogging ideas by just talking to my Mom. Our gossiping and
griping often turn into perfect material for a good post.
Read A Book: I always get inspired to write by reading books. The eloquent words of
the masters makes me want to strive to be a better writer. This sometimes
backfires when I'm trying to read a book, and all I can think about is my own
Just Write: If this was so easy, then you wouldn't have bloggers block, right?
It doesn't have to be full sentences, it doesn't have to flow or make sense, or
even be one single idea. Make a list of potential blog titles. Get anything
that comes to mind out in print. This will often turn into something great.
Read The News: Give a good rant and express yout thoughts on the latest current events.
Timely blogs on news stories are sure to bring traffic to your blog.
Go For A Walk: Walking alone taking in all of nature is peaceful and
therapeutic, it can clear your head and break up some of the nonsense that is
boggling your mind.
Go For A Drive: I usually daydream when I drive. Oftentimes while on a long
drive I'll have written entire blogs in my head.
Take A Shower: Being in a shower is like being in an alternate universe where
for ten minutes nothing and no body else exists. It can recharge your brain and
give you a private space to sort your thoughts.
Post A Picture: A picture's worth a thousand words, right? Describe what you
see, start with a caption or write a poem. Sometimes short posts can say the
Write A Top Ten List: If all else fails you can probably think of your top
picks in any topic.


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Graceland University online astronomy degree
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Info concerning Graceland University online astronomy degree, and registering for elective degree programs. Which college degrees give you the best chances of finding employment after graduation? Given the breadth of engineering specializations, you're likely to find your niche, and be able to design a rewarding career. Concentrating on Graceland University online astronomy degree, for example, you may gain an advantage in job recruiting on-campus. Check with the astrophysics department for course registration deadlines, and space science program requirements. Please use the form above to request admissions info for Graceland University.
Aerospace engineers may design aircraft, space vehicles, satellites, and missile systems. In addition, they test prototypes to make sure that they function according to design. Aerospace engineers are employed in building, analysis and design of commercial aircraft, missile systems for national defense, or spaceships. Aerospace engineers are employed primarily in manufacturing, research and development, and must have a bachelors degree in aerospace engineering or another related field. In addition, aerospace engineers that work on projects that are related to national defense will need a security clearance.
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By Ed Guerrero
From D.W. Griffith's The delivery of a Nation to Spike Lee's Malcolm X, Ed Guerrero argues, the economic movie displays white domination of yankee society. Written with the power and conviction generated through the recent black movie wave, Framing Blackness lines an ongoing epic—African americans protesting display pictures of blacks as criminals, servants, comics, athletes, and sidekicks.
those photos persist regardless of blacks' irrepressible calls for for emancipated pictures and a task within the undefined. even if starkly racist portrayals of blacks in early movies have progressively been changed by means of extra beautiful characterizations, the legacy of the plantation style lives on in Blaxpoitation motion pictures, the wonderful racialized imagery in technology fiction and horror motion pictures, and the resubordination of blacks in Reagan-era movies. Probing the contradictions of such photos, Guerrero remembers the controversies surrounding function offerings by means of stars like Sidney Poitier, Eddie Murphy, Whoopie Goldberg, and Richard Pryor.
all through his examine, Guerrero is responsive to the methods African american citizens face up to Hollywood's one-dimensional photos and superficial promoting of black tradition because the newest fad. Organizing political demonstrations and boycotts, writing, and developing their very own movie photos are one of the kinds of energetic resistance documented.
the ultimate bankruptcy awakens readers to the creative and advertisement leap forward of black autonomous filmmakers who're utilizing video clips to channel their rage at social injustice. Guerrero issues out their various methods to depicting African American existence and hails cutting edge strategies for financing their paintings. Framing Blackness is the main updated serious learn of the way African americans are buying strength as soon as the province of Hollywood on my own: the facility of framing blackness.
within the sequence Culture and the relocating Image, edited through Robert Sklar.
Read Online or Download Framing Blackness: The African American Image in Film (Culture And The Moving Image) PDF
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Twentieth-Century America: A Brief History
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A Gentleman of Color: The Life of James Forten
Winch has written the 1st full-length biography of James Forten, a hero of African American background and essentially the most awesome males in 19th-century the USA. Born right into a loose black relations in 1766, Forten served within the progressive conflict as undefined. by way of 1810 he had earned the excellence of being the top sailmaker in Philadelphia.
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Extra info for Framing Blackness: The African American Image in Film (Culture And The Moving Image)


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2013 Thor Tuscany 40FX
1 / 9
Credit Report:
Used 2013 7,300 miles
Monticello, IN
Model 40FX with a full wall slide + 2 additional slides + Bath & Half layout. Loaded with great equipment, Extended Warranty and Very NICE ! Built on a Freightliner XC Raised Rail Chassis and powered by a Cummins 380 hp turbo diesel, 7,300 low miles, Onan 8 KW diesel generator with auto start feature, 2000 watt inverter, Energy Management System, hydraulic levelers, 3 camera monitoring system, 2 electric patio awnings, dual heat pump A/C units, beautiful "Midnight Ember" full body paint, 10,000 lb. hitch, central vac, exterior entertainment w/HDTV and huge basement storage with sliding cargo tray.The spacious interior features porcelain tile flooring and beautiful Vintage maple cabinetry, a fully equipped frontal galley area with a French 3 door stainless steel residential refrigerator, convection microwave, 3 burner cook top, db. sink, solid surface countertops with a pull out extension, pantry, dining table with 4 chairs and buffet for added storage. This layout offers a mid-coach lounge area with a 84" expanding L-shaped sofa with air mattress, full entertainment with a 40' LCD TV, 5.1 Surround Sound w/DVD, CD, MP3, in-motion auto satellite system and electric fireplace. WOW !Behind the lounge area is a hallway with a half bath, linen closet and enclosed stacked 2-piece washer and dryer. The master bedroom suite has a King Bed, chest of drawers, 32" TV, plenty of storage, on-suite rear bathroom with a large wardrobe, over-sized oval shower, vanity sink and toilet.This coach is further enhanced by a driver's compartment with pwr. leather seating, full instrumentation, buttons galore, one-piece panoramic windshield for a fantastic view, electric privacy shades plus a 4th 32" LCD TV in cockpit area. This baby has it all ! Super clean~no smokers~no pets~no damage or disappointments~Clean CarFax report and covered by a transferable full coach extended warranty. This beauty is located in Monticello, IN. for $189,900. Many additional photos, factory specs and build sheet available by e-mail or by calling The MotorHome Brokers 888-294-XXXX. We invite you to arrange a personal visit soon.
For sale by:
Private Party
October 9 on Oodle
Important Safety Tips
- Meet the seller and test drive before you buy.
- Be cautious of underpriced items.
- Don't wire money or take advance payments.
- Only pay for shipping if you know the seller.
To learn more, visit the Safety Center or click here to report this listing.
More About this Listing: 2013 Thor Tuscany 40FX
2013 Thor Tuscany 40FX is a 2013 Motorhome in Monticello IN. Find other listings by searching for 2013 in Logansport on Oodle Classifieds.
2013 Thor Tuscany 40FX is a 2013 Motorhome in Monticello IN. Find other listings by searching for 2013 in Logansport on Oodle Classifieds.


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Review for Session Manager by macnomad
Rated 5 out of 5 stars
Changed the default folder for saving sessions and when I restarted the browser, lost all my old sessions. How to get my old sessions back ?This review is for a previous version of the add-on (0.7.8.1.1-signed). This user has other reviews of this add-on.
To create your own collections, you must have a Mozilla Add-ons account.


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Whether you are a smart start-up or established business owner, video is the most power marketing tool the industry has used to date.
It doesn't matter what stage, country, or industry I'm teaching in, several entrepreneurs are making the same mistakes that hinder their success marketing through video.
Clarity creates confidence and clarity comes from through education and understanding. It's just like when you learnt to drive a stick shift car; mirror, signal, manoeuvre, it all seemed too complicated but once you have the formula and some practice, you don't need to think about it as it becomes auto pilot.
So, what makes a great converting marketing video? Here is my four-part formula to create killer content videos for your business.
Like anything in business, you must have a vision of the result before you start. This is often called pre-production in the film world. The more you define the vision, the less time and money you will waste.
Not all videos are created equal so choosing a goal for each video is essential. Maybe you want to create some good will content, vlog, or a sales video. You will also need to think about what social media you are wanting to market on.
Related: 3 Steps For Creating Your Own Video Content
Each platform has its limits on the length of video content, for example Snapchat would be 10 secs, Instagram 60 secs and Facebook allows much more. However, less is more when it comes to video content. Try and keep it short and to the point.2
Using organic script writing while incorporating storytelling is a winning factor. For sales videos, I always have a script, but I like to link stories into those scripts. The stories need to add value to the content.
Storytelling is great for connecting the actor to the script so for us entrepreneurs we must open-up a little and show some personality. People buy people at the end of the day, not your product or service.
3Film making
Unfortunately, you are judged by the quality of your product or service. If you are going to venture into video marketing, make sure the quality level matches your current brand level.
Well exposed, steady frame and clean audio are a must have. There isn't an excuse not to produce professional video quality anymore now we all have incredible smartphones in our pockets.
Related: How To Grow Your Business When It's Just You At The Helm
Finally, this is the most important part to the formula. This is where a large percentage of entrepreneurs fail. You can have the best quality video and a great script, but still your video doesn't convert well. You need to learn to connect not just communicate.
The five second subconscious rule stands true. You have five seconds even before you speak where a viewer will decide if they like you and continue to watch. It's human nature to judge, so this is the area to study and develop some new tools for your arsenal.
Tonality, gestures, and body language comes into play. Keep your tone fresh and upbeat, watch your hand gestures and finally make sure you have positive, open body language so you connect not just communicate with your viewers.


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Student Employment Opportunities
The Plemmons Student Union offers a variety of positions for part-time student employment. Please email firstname.lastname@example.com or visit the Student Engagement & Leadership Office, 231 Plemmons Student Union if you are interested in more information about joining our team.
Please send employment application as an attachment to firstname.lastname@example.com.* (Only electronic copies will be accepted). You will be contacted in the event that employment is available. Applications will be held for 90 days.
*If, after completing the document, Adobe will only allow you to save a blank copy, follow these steps: (This will work on any campus computer.)
- On the Adobe PDF Document, click Print.
- For Printer, select "Adobe PDF"
- Click Print
- A Pop-Up Box should appear, allowing you to save the document.
- Save document and email to email@example.com
Click Here for Employment Application for Plemmons Student Union. (PDF Document)
The Department of Student Engagement & Leadership has additional student employment opportunities on the Event Staff at Legends and across campus.
Information and application can be found HERE.
Work Positions
Plemmons Student Union Work Positions
- New employees must be able to work a minimum of 6 hrs. per week.
- Employees can work in more than one area.
Operations Assistant (O.A.)
Working with a crew of other students, this position has primary responsibility for room set-ups, cleaning, & work station substitutions.
Composting Attendant
Works food events in the Student Union to help event waste be successfully sorted into compost, recycling and trash. This position is as needed during event times.
Student Union Desk and Welcome Center
Provides phone listings, campus event and Student Union information to patrons within the PSU and via phone calls.
Game Room
Oversee activities in the Game Room, provide change and perform register transactions.
Films Staff
Responsible for ticket and concession sales as well as projectionist duties during the showing of campus movies at the Greenbriar Theater & Greer Cinema.
Office Receptionist
Screen calls, schedule meetings and greet visitors to the Student Engagment & Leadership & McCaskey Center Offices
AV Technician
Assists patrons in set up, operation, and troubleshooting of audiovisual equipment for events including computers, Data/VHS/DVD projection, & sound reinforcement. Students who are familiar with this type of equipment and have a technical background are preferred.
Ticket Seller
Sells tickets, runs cash register and charge card sales for campus events sold at the Information Desk. Shifts are from 10am-1pm, 1pm-4pm Monday-Friday.
Lounge Attendant
Monitors student and guest use of the Solarium, and lounge space throughout building. Duties include straightening furniture, cleaning & wiping tables, and emptying trash.
Contact Us
Plemmons Student Union
Physical Address:
263 Locust Street
Boone, NC 28608
Phone: (828) 262-3030


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Where Do All the Supplements Go?
April 17, 2011 at 11:53 Leave a comment
Here are the details for the interview:
- Date: Tuesday 19th April 2011
- Time: 8 pm GMT (12 pm PST, 3 pm EST, 9 pm CET, 12.30am IST)
- Place: Online
- Get your FREE subscription to The Gourmet Revolution Show by clicking here now.
Having been a guest on GMTV, This Morning, Richard and Judy and The Terry Wogan show Mary has also been featured in The Times, The Independent, The Daily Mail, The Evening Standard, Metro, Women's Own, FHM, The London Magazine and Health and Fitness magazine. Radio appearances include Women's Hour, Five Alive and James Whales' show.
Mary has also qualified in Polarity Therapy, Cranio-Sacral Therapy, Stress Management Counselling and Bowen Therapy. She has a special interest in detoxification and fasting within The System of Natural Health.
Mary is also a Glyconutritional Wellness Consultant. She educates about and advises upon the use of essential nutrients which positively enable our bodies to experience optimal health
Chrissy White is the presenter to a brand new programme that is part of The Consciousness Revolution Show series. She has a remarkable record of transformational work with individual clients, featured in Vogue Magazine as 'A Gift for Life'. She has a unique way for her clients to connect to their true purpose, passion and vibrant health.
As a Shamanic Healer, Reiki Master, Teacher of Meditation, NLP Practitioner and a Raw Food Consultant, Chrissy has a wealth of experience in the healing art. This whole way of life came about as a consequence of her own experience. All her life she's had a passion for food, especially since discovering how to eat raw living foods as a way to vibrant health and peaceful well being. This journey has evolved over many years. Chrissy has travelled extensively and studied the way that indigenous foods form a major part of natural healing techniques within ancient cultures, especially in The Pacific Islands and South America.
What she tasted and learned there changed her whole relationship with food. Even her love for chocolate has been transposed into love for raw chocolate, 'Food of the Gods' and one of many powerful superfoods offered by nature to enhance our life experience. These are 'lost crops' from ancient civilisations, and include the highest natural source of vitamin C, a naturally low glycaemic alternative to sugar, and an extraordinary root to enhance virility and stamina.
By revealing the fascinating secrets behind foods cultivated and revered in some of the most remote areas of the world, she helps her clients to find their natural weight, develop clear complexions and radiance from within. Their energy levels increase significantly as many long term problems such as migraine, depression, asthma, diabetes, arthritis and chronic fatigue fade away.
Entry filed under: Energy, Gourmet Revolution, Personal Evolvement. Tags: Chrissy White, consciousness, consciousness in UK, diet raw food, dietary supplements, food nutrition, food supplement, food supplements, Mary Chase Hopkins, nutritional supplements, raw food, raw food diets, raw food recipe, raw food recipes, raw foods diet, supplements, vitamins and supplements.
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Contact Info / Websites
Recent Movie Reviews
The Mockingbird Song
Rated 5 / 5 stars
very original
its not exactly what you would describe as a conventional bed time story, it might not be as well received by a child being tucked into bed, but i tought this was an excellent story that was well animated, you really get to know the main character in this short amount of time, i liked that as well thats why i give it a 10
The Only Thing I Know
Rated 5 / 5 stars
you ary correct !
Although i love video games, i always keep in mind that they are purely entertainment . nothing more. I would much rather spend my time bettering myself in some way . and i only play games once all priorities are taken care of , i do not spend hours on end playing them,and i'd much rather play with a friend . Like i said there entertainment . nothing more. your message is clear and more gamers should think like this
Deadly Bite
Rated 4.5 / 5 stars
that is the greatest interpretation of a classic joke i have ever seen
Recent Art Reviews
Jellyfish Heaven
Rated 5 / 5 stars
a few questions for the artist
1. who did you paint this for
2. do they like jelly fish
3. do you like jelly fish
4.is there a jelly fish hell
5.what do jellyfish do in jelly fish heaven
6.do you think i am taking this art work a little too seriously
i would love to here back
as for the art work. very calming and creative
1. I painted this for my girlfriend.
2. Yes.
3. Yes.
4. Not that I know of.
5. They pretty much just float around all day; there's nothing jellyfish enjoy more than aimlessly floating about.
6. Of course not!
Thanks for your review.
Rated 5 / 5 stars
so this is the highest rated pic in the Newgrounds art gallery. impressing


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You know those crazy times in life where things work out in the strangest ways and all you can do is sit back and think: "Man, I have been so blessed" and then you wonder how things can work out so perfectly?
The last few days have been that way for me and I feel like the most I can do right now, is express my gratitude.
First of all, I am thankful for my schooling and how it is all working out right now. Anything from classes, to credits, to financial aid, it is all coming together. I am thankful for the knowledge that I have gained and for my progression. Just one year ago, I knew nothing about the things I would be learning. I only knew I was determined to reach my goal. I am so thankful for this past year (and almost one half) and everything I have learned.
I am thankful for my job and the HUGE blessing that it is in our lives. We have been so blessed as far as jobs go, we are both doing things that we really truly enjoy, even though they are jobs just to get us through to our careers.
I am thankful for our faith and the foundation that it is built on. I am so thankful for the opportunity each day to correct the mistakes I made the day before and become better. I am so thankful for the plan of salvation and for temples. Because of these two things, I can spend forever with Josh, not just this life, but also the next.
I am thankful for family and the love and support they show us each and every day. I am thankful for my wonderful parents and everything they have taught me so I can be the person I am today. I am thankful for my siblings and our adorable nephews Brody and Krew:
They light up our lives and we miss them so much. It has been so hard to have them all the way in BUFFALO, NY. We love them to death and I am so thankful we can still talk to them or see them at the push of a button. I am so thankful for our soon-to-be niece! My sister Ashley is having a girl and we can't wait to spoil her to death, then send her home:]
Remember this post? Well, I am thankful for this stage of our lives. I am thankful that Josh and I have the chance to go to school and have this time to prepare for the future. It is so fun to see my sisters starting and enlarging their families! That time will come for us too, we just need to enjoy this time while it lasts. I am thankful for this time together.
Last but not least, I am so thankful for Josh and the wonderful man that he is. He makes me so happy and I am so thankful that I found and fell for him. He takes such good care of me and we help each other become better people. Thank you for being a wonderful husband Joshie, I love you more than anything!
So, what are you all thankful for, I promise as soon as you start looking around, or even listing them, you will find even more ways than you thought how you have been blessed. Try it.


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chicago vegan dining guide.
the past couple months, i've noticed an increase in messages from people coming to visit chicago & looking for recommendations for vegan restaurants. sooo ... i decided to put together a little dining guide of my favorite places in the city.
let's keep in touch! instagram. facebook. twitter.
so after a day of this ...
& this ...
& obviously this ...
here are my favorite places to grab grub ...
first & most importantly ... my top vegan/vegetarian only joints :
- chicago diner : the true vegan chicago staple.
- quesadilla la reina del sur : 100% vegan/vegetarian mexican food. "carne asada" on fleek.
- kitchen 17 : the k burger & pizzas are legit. cons = sometimes they run out of menu items.
- handlebar : my fave spot for vegan brunch + awesome buffalo wrap if you do lunch/din.
for pizza :
- lou malnati's : don't trust anyone who says giordano's is the best chicago deep dish. does not compare to lou's.
- forno rosso : my new fave for neapolitan 'za + in the ultra trendy west loop.
- the boiler room : two thumbs up for vegans & non-vegans alike. with great drink specials.
asian cuisine :
- coast : bomb sushi + byob.
- saigon sisters : one of my favorite spots for pho in the city.
- wasabi : legit vegan ramen.
- slurping turtle : for frequent top chef master takashi spottings.
- le colonial : go for the spring rolls ... stay for the spring rolls. (also, separate vegan menu!)
quick bites :
- le pain quotidien : so much vegan stuff ... that is also 100% organic.
- left coast food + juice : again, so much vegan stuff (the tostadas are my jam) + juice!
- lyfe kitchen : i know this is a "chain," but if you're coming from oklahoma, you may not have had it!
- doc b's fresh kitchen : get the california salad, add tofu, sub carrot/ginger dressing, thank me later.
- athenian room : my favorite hangover food ... a salad covered with fries & greek dressing. come on.
nicer spots :
- girl & the goat : high-end dining with a separate vegan menu! (best shishitos in the city.)
- bad hunter : no words. just go.
- lula cafe : vegan options for those looking for something a little more hipster.
- monteverde : give me all the pasta.
- leña brava : an impossible to get into rick bayless spot.
dessert :
- sprinkles : again, i know there are lots of these. but you cannot beat the vegan red velv. i'm sorry, but you can't.
- pie pie my darling : can be found at handlbar & misc. other vegan shops in the city. SEEK THIS OUT.
- stan's donuts : dessert, breakfast, whatever.
resident chicagoans ... what am i missing?! any glaring omissions?? other category suggestions?? maybe ... an "off the beaten path" list? feel free to weigh in!
visitors ... enjoy!!
let's keep in touch! instagram. facebook. twitter.


text: Cast Marko as Barney Boley as BJ Lyle as Riff Georgie as Carlos Retrieved from "http://scratchpad.wikia.com/wiki/Fuzzaboom/Barney?oldid=2291812" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Fuzzaboom Barney Barney parodies


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Faster than a speeding carrier pigeon:
About the Photographer
Madison is an editorial and commercial photographer in Seattle, Washington. As an Arizona native, she often travels between the two states to accommodate her clients in both regions (read: wannabe snowbird). In her spare time, you can find this desert rat beach combing, making art, obsessing over plants or chasing the Milky Way. Favorite topics include: the plight of sea turtles, travel, outer space, conservation, unusual animal facts, and cats.
Publications/Clients Include:
- Associated Press
- Humane Society of the United States
- New York Times
- Seattle Times
- Washington Post
- ProPublica
- Shell
- Phoenix Magazine
- Grand Canyon West Resorts
- Baltimore Sun
- The Advocate


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Sneak Peek of Vermont!
Remember from my previous Wisconsin post I had mentioned that I had been doing some traveling? Well, Wisconsin was the first visit and then 4 days later was Vermont. We have been blessed to be able to go visit the beautiful state for about the last 4 or 5 years. I love photographing the small little towns, old structures, gorgeous landscape and the awesome fall leaves.
Here is just a peek……….with more to come!!!
Filed under: Travel Show Comments |Add a Comment
Especially when I have a beautiful young lady like this to photograph!! You guessed it………this is in Wisconsin too!! I think I could have stayed there at least another week! So many awesome places to shoot! Sigh! This gorgeous lady was a delight to shoot…….thank you K. Here are a few for you!!
They grow them well in Wisconsin! This beautiful girl was so much fun to shoot! Not only is she a great model put a heck of a swimmer as well! I hope you enjoy this one A!!!
What a great day this was!! I had so much fun capturing these two boys doing what boys do!! The Wisconsin weather was great that day and the frogs were all muddy and slimmy, just like little boys like them!! If I wasn't capturing a fun moment with my camera, I was pulling the youngest out of the mud………I didn't mind a bit, it was so much fun!! My new shoes washed off perfectly fine and we all had smiles on our faces!! I Can't wait to go back and do this again sometime!!
I have been doing some traveling!!! First it was to Wausau Wisconsin ( I can't say or think wausau with out doing a cool karate kick with my leg kicking up in the air and a cross chop with my hand, weird I know!!) Anyway, I went to visit my very special friend and professional photographer Jenny!! All of you in Wisconsin are so lucky to have her!! Jenny and I did some awesome and cool photos shoots with some kiddos (that I will be posting in the next few days) and we drove all over the beautiful country side looking at the gorgeous fall colors…………I was in heaven!!! Thank you Jenny girl!! Here is one of my faves!!!
Filed under: Personal, Travel Show Comments |Add a Comment