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Ghazi Will Be One of Its Kind: Taapsee Pannu
To be helmed by Sankalp Reddy, the film, which also stars Rana Daggubati, is touted to be India's first submarine film.
Image: Instagram/ Taapsee Pannu
New Delhi: After her latest release Pink, which won critical acclaim, actress Taapsee Pannu is gearing up for the release of her upcoming movie Ghazi which she says will be one of its kind.
"I am very proud to a part of that film ('Ghazi'). It's going to be one of its kind... India's first submarine film based on a real incident of how the (Pakistani) Ghazi submarine was sunk," Taapsee told IANS over phone from Hyderabad.
To be helmed by Sankalp Reddy, the film, which also stars Baahubali: The Beginning star Rana Daggubati, is touted to be India's first submarine film which will be about the sinking of PNS Ghazi, a Pakistani submarine.
Talking about her role in Ghazi, the 29-year-old actress said: "I play a Bangladeshi refugee and the film is due to release soon. I think it is undergoing some graphic work... It is the only period film I have done so far."
But it is unlike other period dramas, she said.
"It is not a regular costume drama period film. It is something really interesting," added Taapsee, without divulging any more details.
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subset: commoncrawl


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By Indian Institute of Technology, Delhi (I.I.T. Delhi)
The IIT Delhi OSA Student Chapter hosted OSA Ambassador Dr. Arti Agrawal from City University, London as a Traveling Lecturer. The talk was on Finite Element Method for Photonics and Optics. She covered some of the key principles of numerical methods (for photonics) and shown some examples of different classes of methods. She explained the basics of FEM and steps involved in the method, with some examples. She also discussed the principle of writing code for such a method as well as some tips on best usage of commercial software. The talk was attended by students from different background like Electrical Engineering, Engineering Physics and pure physics who belonged to all levels like BTech, Mtech, PhD etc as well as faculty members. The talk was very helpful for the students at our institute as it gave them insight into the applications of commercial software based on FEM in their respective research areas. She also interacted with the chapter members personally and gave them advice on their future research maneuvers. She also gave new and interesting ideas for our future chapter activities. Overall, the traveling lecture was a successful event for the students. Thank you to OSA for providing the Traveling Lecturer Program.
Posted: 6 September 2016 by
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How To Get Tested For Std Byhalia MS 38611
It is a known medical truth that infection can be carried through a number of modes of transmission readily available to the disease by the orifices and membranes of the body. Usually than not, the least most likely gone over and typically prevented by lots of individuals understands the truth of a sexually transmitted illness, its mode of entry and how to handle it. In this age, the occurrence of sexually transmitted illness is at its peak, yet there are still a lot of taboos relating to seeking treatment and finding out about Sexually Transmitted Disease symptoms and getting Sexually Transmitted Disease screening at private Sexually Transmitted Disease testing centers and clinics.
A viral, fungal or bacterial infection can be transmitted through intimate contact. Naturally there might be numerous types of infection which can be transmitted via genital contact; the meaning of sexually transmitted disease is separated to conditions that depend on sexual contact for its transmission and propagation. Venereal illness is a terms of comparable meaning however is normally connected with five generally acknowledged illness.
STD or Sexually transmitted diseases can also be captured non-sexually but for most adult infection cases, the early infection caused by contamination through an intermediary driver such as towels, toilet seats or bathing facilities is practically nonexistent.
The medical meaning of a Sexually Transmitted Disease limits it to a description of more than a cluster of 20 numerous infections brought about by the exchange of exudates, or internal fluids such as blood, semen and by means of direct physical contact with affected carriers of STD's.
The start of adolescence is an opportune time where several health danger behaviors are established and can be a window for exposure to a handful of sexually sent infections. Health jeopardizing practices during the teen stages increase the rate of sexually transferred disease transmission dramatically.
Different instances such as unsafe sexual relations, alcohol and restricted drugs experimentation are thought about normative habits for adolescents. Of course these acts result as a health threat and promote the acquisition of sexually transferred disease. This results in numerous individuals in these market showing Sexually Transmitted Disease signs early on and on numerous celebrations.
The acquisition of STD at this normative stage can result to severe health repercussions that alter the reproductive course of a carrier's life, such as sterility, infertility, neonatal transmission, numerous forms of cancer and even worse, AIDS.
STD Signs that might be neglected can be any of the following manifestations:
- A teen who experiences bleeding may think it is an extension of her period and does rule out Sexually Transmitted Disease testing until other signs appear such as vaginal burning and irregular genital discharge.
- Teen males might believe that a discharge from their penis might be a result of bad hygiene or pre-cum however when accompanied by an unpleasant burning experience and difficulty in urination must be prospect for a consultation.
- Men and Females establish rashes as part of symptoms connected with numerous STD's but are typically not thought about a market by numerous in the teen phase in view of something less serious such as a case of the pox or measles. Sexually Transmitted Disease screening ought to be advised if the prospect has already experienced pox or measles but display "like" symptoms.
The management and prevention of STD can be summarized in 2 phases. The very first phase includes a dedicated details project that stretches guideline about sexually transferred disease and Sexually Transmitted Disease testing on all compasses of the education system in both personal and federal government sectors. The 2nd phase is a mindful effort of both prospective carriers and their member of the family to stay attuned to health risk habits that may promote a STD infection and acting vigilantly to deal with and handle the disease.
There are a number of extremely qualified and well reputable personal centers that promote privacy and discrete management of sexually transmitted diseases especially for adolescents.
Sexually Transmitted Disease Testing in the house Has Advantages in 38611 Mississippi
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There are std screening kits that can be completed in your home at your own risks. Totally convenient for house screening, doing it yourself means doing all the work and research study to make sure the test is done correctly. If you do have a sexually transmitted disease according to the test, be sure to contact your doctor for a visit.
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If you are in requirement of talking to somebody about std testing or about where to discover complimentary centers for std screening, go online to online forums. People like to talk to others in forums and many will be pleased to provide out details they currently have or refer you to resources they might have access to.
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There are cases that have actually been talked about on the getSTDtested.com website where patients went to their physician for std testing and were rejected. Some physicians have their own view on testing for such diseases and may feel there is not a requirement, and regrettably it can occur. When you do the std house testing, you will have the opportunity of making certain it gets done and no one can refuse you.
Do not let a disease scare you far from getting evaluated. Now that you can take advantage of sexually transmitted disease screening in your home, you should not be stressed that you might be living with something that could make you self mindful.Where Do You Get Tested For Stds Byhalia MS 38611
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"I am retired and interested in photographing surfing, glamour and fashion."
"I love capturing the stoke of surfing both for myself and other surfers."
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"Hello - I enjoy capturing surf images and working the images to suit my imagination."
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"Hi my name is Nathan and I love surfing and watching the waves come in over and over!"
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Reader: 3/25/2009>
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Dr. Lin: 3/25/2009>You had exhausted your hypothalamus-pituitary-adrenal axis and your cholinergic/serotonin/GABA nervous control, and severely abraded your prostate/urethra/bulbourethral glands by chronic over-ejaculation (high-frequency ejaculation) with your first girlfriend, leading to chronic excessive hypothalamic and adrenal dopamine-norepinephrine-epinephrine conversion for sympathetic nervous Fight or/and Flight responses and excessive inflammatory hormones prostaglandin E2 and histamine production. A chronic high level of norepinephrine and prostaglandin E2 in the brain alternates your brain and nervous function with positive (enhanced) neuroplascticity for f the noradrenergic, adrenergic and sympathetic nervous systems, but negative (depleted/destructive) neuroplasticity for the dopamine, cholinergic/vegal/parasympathetic, serotonin and GABA nervous systems.
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Discussion in 'Pop & Justice' started by mindtrappa, Apr 21, 2017.
Okay, from the viewers standpoint I disagree.
Joanne during the AMAs would be much more preferred, but oh well, do the one off single that came out months ago and has run it's course and doesn't have a parent album attached to it I guess.
A Joanne medley akin to Beyoncé's Lemonade showing at the VMAs should really have been quite a moment, but streaming from the tour wouldn't have logistically made that possible.
I agree with @RJF that, despite its amazing success and all it's given us, as a piece it has been sold a little short. It really is an album that could have had so many visuals attached to it, her writing and the production was so rich and varied on this one.
General public / fans in "not thinking from the Grammy committee perspective" shocker
Weren't you all begging for a TV performance of The Cure from the first night we saw the choreo?
Does anyone else deeply prefer the "Million Reason"'s album version melody as opposed to when performed live...or is it just me...?
But the live version doesn't have Hillary Lindsey's bleating in the background
I'm not sure how performing a song that was a single 8 months ago is any less "messy" than doing the one that might be up for a Grammy nomination if that's the reasoning we're going to take.
What are the chances the AMAs know that if they get Gaga on, they'll get more viewers, so they asked her and because she has nothing new to show off, they just decided tour footage of her newest song is the only thing they can give them? lol.
... because she's already performed it. At the same award show... a year ago. I'm not sure what about that is so difficult to grasp.
She never performed The Cure anywhere, and Million Reasons has had it's time too (a year ago at the same event). As someone watching it makes the most sense and it's a more refreshing performance.
The AMAs probably wanted her to perform a recognizable song that people already know
So often with her songs in the live versions she changes it up and I think they sound even better. Poker Face's "I want you to know" will always stick with me.
Come to mention it, I vaguely remember her saying Hillary wasn't keen on a specific key or something from Million Reasons' final chorus that Gaga wanted, and Gaga proceeded to sing it that way live anyway.
Can we really consider The Cure as a song everybody knows though...
I don't care what she performs. The fact that she is performing is enough. And considering "The Cure" is one of her best singles, I wouldn't mind a performance of it.
Yeeeeeah still not seeing the difference. The general public isn't going to remember what she did a year ago, or if she even performed there at all.
I don't care what she does, I just don't see why @RMK thought it'd be a messy choice if she decided to perform "Million Reasons", and was offering up a reason for why she possibly would and why it shouldn't be looked down on if she did decide to. I don't see what about that is so difficult to grasp.
She may even surprise us all and just do "John Wayne"
Or a new single!
There's playing devil's advocate and then there's this. Give over.
All the AMA promo on twitter right now for her performance includes footage from last year's performance of Million Reasons, so it would at least be fresh in the internet's collective mind. The GP doesn't have to remember what she did last year for a Buzzfeed journalist to remind them what it was, and then the press isn't as good.
I don't think a MR performance makes sense for this event, Grammy nomination or not. Homegirl has no shortage of songs and Joanne/The Cure stand out to me as two obvious choices, what with the Five Foot Two connection. There's a decent level of public awareness of her fibromyalgia now (had at least two colleagues and a relative bring it up), and The Cure in particular is a great way of acknowledging that (even if it was written about Sonja) while also showing non-concert-goers that she's still up and fighting.
I think it's messy to perform the same song at the same award show two years in a row. Maybe it would be cool to give Million Reasons another push, but as a fan or viewer it's always nicer to see something new. You don't have to agree.
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text:
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1 dormitorio
1 cama
1 baño
2 huéspedes
1 dormitorio
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El alojamiento
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Estancia mínima de 3 noches
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Before our arrival to Plymouth, everything was fine, Becky had been fantastic with me, very cooperative and polite. But the nightmare started upon arrival in Plymouth. The address of the house wasn't correct, Becky didn't know the correct street number of the house, so first she sent me to number 9, then she had to call someone to check the number of the street because she didn't know it by heart and she told me it was number 8, not exactly one close to the another, not even the name of the street corresponds to what shown on the website, it's not North East Road, but Winston Avenue. They are close but the address isn't correct. Probably the house belongs to an estate agency and is not Becky's. I had to call her 8 times overall to have the correct address of the house and get the key. She never showed up, not during our stay. I went up and down a hill with all the suitcases and a child of 4 years old several times and had to wait outside some time to get in the house. The house was very noisy, the lady living downstairs put the hi-fi very loud, especially during the weekend. When not putting music very loud, she sang herself, even at 11 p.m. at night or shouted. It is like a terraced house which has been divided in three small apartments. So you can hear all the sounds, there's no isolation. The entrance door to the flat is very "thin" and you cannot lock it so anyone can easily enter the house pushing the door down. The police came 3 times in 21 days, looking for two different people living just downstairs, it had never happened to me in the past. I was scared because I was living alone with my child. Lots of quarrels and shouting from downstairs. I had asked for some sheets and towels, because I had no room in the suitcases and considered that this was part of the renting as in many other houses. She only gave us the ones on the bed, not any spare ones. Quite uncomfortable, I had to wait to wash them and put them in the dryer and put them back in the bed. The house only had a TV channel, BBC 1. We basically had no TV for 3 weeks, not nice with a 4 years old child who was looking for some cartoons. The coffee machine was old and all rusted and it wasn't possible to use it. I couldn't have a coffee for 3 weeks. The kitchen wasn't well-equipped at all and it was dirty. Only few plates and some cutlery are in the house, nothing more. There was only a small carpet in the kitchen for the sink. The bathroom had no carpet so we had to move it from the kitchen to the bathroom every day and vice-versa.
Our experience was not entirely satisfactory. A week before arrival our host forced a shift to another apartment which - despite being brand new - was completely anonymous, placed in a dark lane and with a view to a concrete building only. Second we did not feel much wellcome. Host was not present on arrival - we had to call - and flat was with no coffee or tea. Even soap was missing, and towels and toilet paper (!) in short supply. This is the first time I have rentet accomodation in the UK with no coffee and tea.
Respuesta de Becky:
Due to a problem with the original apartment i went out of my way to offer another apartment in Plymouth although i could have cancelled i did not want to let the couple down and offered Steen and his wife a more expensive apartment alternatively , there was a choice of two apartments , one with a sea view but the client didn't like the location. I went to the apartment to greet Steen and was very polite and kind to them and showed them everything in the apartment and the free parking space . The apartments are 5 minutes walk to the Citu centre and the Barbican area and are very popular with guests The apartment is a very high standard with modern bathroom and kitchen and king size bed The couple were supplied with towels and plenty of loo paper and asked for more even though they were only staying 4 nights. I drove across town to bring them more towels and loo paper. They sent me a message asking for tea and coffee but i said that the guests normally bring their own. I would have taken tea and coffee as they requested as nothing is ever too much for me The apartment is not on a dark street it is well lit and frequented by many guests who stay in the apartments and the apartment is secure and safe with free parking. Having made every effort to offer an alternative of a good standard and be concerned about letting someone down this has made me think twice !
Agosto de 2015
I was born in Plymouth and know the area well
I am fond of travelling, art,theatre,cooking and swimming
I work at helping others enjoying travelling to this lovely city of Plymouth and have written an extensive guide to the area.
Its great to welcome visitors to Plymouth !
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Featured Books, with extracts, by Joyce L Brisley
Milly Molly Mandy Stories
Joyce L Brisley
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The effects of microgravity on T lymphocytes was studied on three sounding rockets flights with µg phases lasting 7- 13 min. Automated pre-programmed instruments could inject fluorescent-labelled concanavalin A and/or para-formaldehyde as fixative at given times. A microscope remotely controlled from the ground and connected to a CCD camera recorded cell motility and interactions under µg. Chemically fixed cells were labelled after flight with monoclonal anti-vimentin and anti- tubulin, respectively, followed by fluorescent anti-mouse Ig. First, while binding of concanavalin A to the cell membrane is not affected, patching of the receptors is slightly but significantly retarded during µg. Second, marked alterations of the intermediate filaments of vimentin as well as of the microtubule network are observed after 30 s of µg and change only slightly during the flight. Ground controls conducted at hypergravity show that the changes are not due to the acceleration peak of 13-15 g following launch. Third, cell motility and cell-cell contacts also occur when cells are free- floating in µg. These results indicate that direct effects of µg on the cytoskeleton are likely whereas little changes occur at the membrane level, and are in agreement with recent data showing that gravity controls the pattern of microtubule formation. The existence of active movements and cell-cell contacts supports, but does not prove, the notion that T cells can communicate and transmit signals in µg.
A dramatic depression of T lymphocyte activation in vitro by concanavalin A (Con A) was discovered in an experiment conducted aboard Spacelab 1 in 1983.¹ More sophisticated experiments followed during Spacelab D1 in 1985,² SLS-1 in 1991³,4 and IML-2 in 1994.5 All investigations confirmed the effect. The results indicate that the depression is mainly due to a lack of expression of the interleukin-2 receptor.4, 5 Conversely, when T lymphocytes and monocytes were attached to microcarrier beads, activation was more than doubled under µg compared with controls cultured at 1 g, either in flight or on the ground.3, 4 It was concluded that signal transduction is markedly changed in µg.
The work described in this paper was carried out to clarify aspects of such effects that can be investigated during relatively short µg periods (7-13 min) achieved on sounding rockets using either automatic pre-programmed instruments or a microscope adjusted via telecommand.
The first question addressed was whether binding of Con A to the cell membrane, followed by patching and capping of the membrane proteins involved, would take place normally in µg. It is believed that this is the first signal required for activation.6 Patching and capping are processes occurring within a few minutes after binding. Abnormalities in this process would obviously alter the transduction of the first activation signal. The hypothesis was that exposure to µg could produce changes of certain membrane properties.
The second question was related to structural changes possibly occurring within the cells exposed to µg. Gravity unloading per se is not sufficient to change the shape of a cell, because the microenvironment is subjected to forces that are many orders of magnitude larger than that of gravity.7, 8 However, organelles with a higher density than the cytoplasm (such as ribosomes, centrioles and nucleolus) may exert at 1 g some pressure on cytoskeletal and other structures. This pressure would disappear in µg. A similar effect occurs in the statocytes of the roots of some plants where gravitropism is attributed to the pressure of the statoliths on the endoplasmic reticulum. Structural changes may have an important impact on cellular events. In addition, their occurrence would demonstrate the existence of direct effects of µg on single mammalian cells.
The third question was whether free-floating cells in µg are capable of autonomous motions and, hence, of cell-cell interactions. Cell-cell interactions are an important means of cell communication and signal delivery in T lymphocyte activation. Although in previous experiments cell aggregates were always observed in samples fixed in µg,¹,² one could argue that lack or reduction of cell contacts is the cause of the loss of activity in space. Direct evidence in real time will certainly give a clear answer to the question.
We present here the results of four experiments carried out on the MASER 3, MASER 4 and MAXUS 1B sounding rockets. In MASER 3/4, the binding of fluorescent-labelled Con A (FITC-Con A) to human lymphocytes and the subsequent patching and capping was investigated. In MAXUS 1B, one experiment studied the structure of the microfilaments of vimentin and of the microtubule network in Jurkat cells (a human T cell line) chemically fixed in-flight and labelled after recovery with monoclonal antibodies. In the second experiment, motility and interactions of human lymphocytes were recorded in real time by an onboard microscope. A detailed description of the instruments aboard MASER 3/4 is given in ref. 9, while the equipment used on MAXUS is presented in ref. (6).
Flight instruments
All cell cultures were incubated at 37°C during flight. In MASER 3, the experiment was installed in the CIS-1 (Cells in Space) module developed by Fokker Space & Systems with the National Aerospace Laboratory and the Centrum voor Constructie en Mechatronica (CCM) in The Netherlands. The experiment-specific device consisted of a unit provided by CCM. Cell cultures and reagents were stored in three contiguous sections (respectively, fixative/cells/Con A) of a flexible silicon tube closed at both ends and separated by roller bars released at pre-programmed times. See ref. 9 and Section 3 of this volume for details.
In MASER 4, a more sophisticated system, the Plunger Box Mix Unit (PBMU, also provided by CCM), was used in the CIS-2 module. The reagents and, later, the fixative were injected into the cultures by pre-programmed plungers.9
Two instruments, both integrated in the TEM 06-5M module and provided by ERNO Raumfahrttechnik, Bremen, were used in MAXUS 1B (see ref. 6 for a detailed description). To study the cytoskeleton, cell cultures and fixative were sealed in two disposable syringes separated by a valve. At pre-programmed times, the piston of the syringe with the fixative automatically injected the reagent into the culture. The observation of cell movements was performed in a glass cuvette mounted in a phase contrast microscope connected to a CCD camera. To prevent sedimentation of the cells, the cuvette was rotated at 30 rpm/min during the pre-flight and launch phases; rotation was stopped at the onset of µg. In-flight, the selection of the observation field and focusing were operated manually via telecommand from the ground station.
Each flight experiment with the exception of the observation of cell movements was accompanied by a synchronous ground control with the same batch of cells in an equivalent apparatus.
Human lymphocytes
Peripheral blood was drawn from healthy donors 14-18 hr before launch. Cells were purified by gradient centrifugation on Ficoll (Pharmacia, Uppsala) and cultures were prepared as described previously.10 The preparations contained 84±3% of lymphocytes. The remaining were macrophages (13±3%) and polymorphonuclear cells.
Jurkat cells
Jurkat cells, derived from a human T cell leukemia,11 were kindly provided by A. Lanzavecchia of the Basle Institute of Immunology, Basle. The cells are mycoplasma-free. Cells were grown in RPMI 1640 medium supplemented with 10% (v/v) foetal bovine serum (Biochrom KG- Germany), 20 mM HEPES, 5 mM sodium bicarbonate, 1 mM sodium pyruvate, 4 mM L-glutamine and gentamycine (50 µg/ml).
Monoclonal antibodies anti- vimentin (V-5255) and anti-ß tubulin (T-4026), and anti- mouse polyvalent immunoglobulins FITC-conjugated (fluorescein isothiocyanate) were obtained from SIGMA (St. Louis, Missouri, USA). Con A-FITC was purchased from ICN Immuno Biologicals (Lisle, Illinois, USA).
Flight investigations
In all investigations, the experiment-specific units containing the cell cultures and reagents were installed in the modules of the rockets 2 hr before launch. The µg phases of MASER 3 & 4 lasted 7 min, that of MAXUS 1B 12.5 min. µg in a sounding rocket flight is defined as g <10-4. The flight samples were recovered and handed over to the experimenters within 90 min of launch.
In MASER 3, lymphocyte cultures (290 µl, 2x106 cells/ml) were sealed in the middle part of each of the eight silicon tubes of the flexible tube unit. After onset of µg, FITC-Con A (280 µl, 51 µg/ml, final concentration 25 µg/ml) was added. Fixation followed by mixing with paraformaldehyde (final concentration 1%) 0.3, 30.3, 60.3, 90.3, 120.3, 180.3, 270.3 & 390 s after the addition of FITC-Con A. The protocol of the investigation in MASER 4 was analogous to that of MASER 3. In four of the six chambers, FITC-Con A was released 180 s after onset of µg; fixations followed 0.3, 30, 90 & 210 s later. In the other two chambers the mitogen was added after 300 s of µg; fixations followed 30 & 90 s later. All samples were duplicated.
In MAXUS 1B, the labelling of vimentin was carried out on Jurkat cells fixed 30, 420 & 720 s after the onset of µg by means of an automatic pre-programmed device. For tubulin labelling, the cells were fixed after 30 & 720 s. To 1 ml of culture of Jurkat cells (1.7x106 cells/ml), 1 ml of 2% paraformaldehyde was added. The observation of cell motility was made in cultures containing 3x106 lymphocytes/ml. Realtime recording was on magnetic tape in an onboard CCD camera. Analysis was performed on a papercopy (video-printout) showing pictures taken at 13 s intervals.
Evaluation of binding, patching and capping
After recovery, the cells were washed three times with Hanks balanced salt solution (PBS) and fixed on microscope slides by cytocentrifugation (200 g, 8 min). The cells were mounted in glycerol containing p-phenylendiamine as antifading and observed in a Nikon Optiphot microscope. At least three slides were prepared from each sample. About 500 cells were evaluated and counted on each slide. For statistical analysis of the data the one-tailed U-test of Wilcoxon, Mann and Whitney was applied.
Labelling of vimentin and tubulin
After recovery, the cells were rinsed with 10 ml of cytoskeleton- stabilising buffer (SB) containing 50 mM imidazole, 50 mM potassium chloride, 0.5 mM magnesium chloride, 1 mM EGTA, 1 mM 2-mercaptoethanol, 4 M glycerol, pH 6.8. The cell pellet was resuspended in 1 ml of SB containing 1% Triton X-100 and the cells were kept for 30 min at ambient temperature. The cells were rinsed again with 10 ml SB, pelleted and incubated for 30 min at ambient temperature with 200 µl of anti-vimentin or anti- tubulin, diluted 1:100 with SB. At the end of the incubation the cells were rinsed with 10 ml of SB, and the cell pellet incubated with the secondary antibody FITC-conjugated, diluted 1:100 in SB. Incubation and rinsing with SB followed as above. Finally, the cells were cytocentrifuged (200 g, 8 min). The cells, adhering to microscope slides, were mounted in glycerol containing p- phenylendiamine as antifading and observed in epifluorescence in a Nikon Optiphot microscope. Three slides were prepared from each sample and about 400 cells were evaluated on each slide. Micrographs were made on Fujichrome 400 ASA films. For statistical analysis of the data the one-tailed U-test of Wilcoxon, Mann and Whitney was applied.
Binding of the mitogen
The type and number of signals required for T lymphocyte activation have yet to be clarified and are still a matter of controversy.6 Nevertheless, there is agreement in the literature on the nature of the first signal. This is usually provided by the presentation to the T cell of the modified antigen in conjunction with the MHC component of the antigen- presenting cell, usually a monocyte. The presentation of the antigen can be replaced by a mitogen such as Con A. At least part of the effects observed in space could be attributed to alterations of the binding pattern. Therefore, it was essential to establish whether the binding of Con A to the cell membrane, followed by patching and capping of the receptors involved, occurs normally in µg.
Two experiments were conducted to answer this question; preliminary data were presented in conference proceedings.12, 13 In the first investigation (MASER 3), Con A was added to the cells immediately after µg conditions were established. Fixation with paraformaldehyde followed after different preset times of 0.3-390 s. No significant differences in the rate of binding, patching and capping compared to the 1 g control were observed.12 Binding was very fast and completed after 30 s.
The second experiment (MASER 4) was carried out to test if the lack of differences between flight and 1 g control samples arose from a lack of influence of µg or from the fact that the cells did not have sufficient exposure time to 'adapt' to µg. Therefore, Con A was injected into the samples 3 & 5 min after the onset of µg, followed by fixation after different preset times. The data obtained confirm that, even after 5 min in µg, there are no changes in binding of Con A to the cell membrane compared to the ground control.13 Furthermore, there are no differences in the patching of the Con A receptors in the samples where the cells have been exposed for 3 min to µg before the addition of the mitogen (Fig. 1). However, patching is significantly retarded when Con A is added after 5 min of µg (Fig. 1), especially in the samples where the cells were fixed 90 s after the addition of Con A. In the flight samples, 2.9±0.9% of the cells show patching, compared to 7.4±3.4% in the 1 g control (99.5% significance of the difference; U(11.6) = 5, alpha = 0.005). In the samples fixed after 30 s, the difference in patching is statistically significant at the 95% level (1.9±1.0% in the flight sample, compared to 3.9±2.6% in the ground control; U(12,7) = 19, alpha = 0.05). Furthermore, we also observed a significant difference in patching between the samples exposed for 3 or 5 min to µg and fixed after 90 s (97.5% significance level; U(6,6) = 3, alpha = 0.025). In addition, the data in Fig. 1 indicate that, despite a significantly lower rate at µg, patching is a dynamic process that would be completed if the incubation time were longer than 210 s. The question on differences in capping could not be answered with statistically significant data as the number of cells showing capping was too low in this experiment. However, there seems to be a tendency that capping is retarded in the flight samples.
Fig. 1. Patching and capping of Con A receptors following binding of the mitogen to human lymphocytes. The bars represent the percentage of cells showing either patching or capping at different fixation times after the addition of Con A. Standard errors of the means are given.
The fact that the patterns of binding, patching and capping are not changed compared to the ground control when Con A is added immediately after the onset of µg, and the fact that changes in patching - but not in binding - are observed only when Con A is added after 5 min of µg, indicate that the short exposure to hypergravity during the launch phase is not relevant.
The data show that the influence of µg on the delivery of the first activation signal is rather small and that, therefore, rapid processes such as binding of the mitogen, patching (although slightly retarded) and probably also capping are not involved in the depression of the in vitro activation of T lymphocytes observed in the Spacelab experiments.1-5
In connection with the study on cell movements in µg, we investigated the occurrence of cytoskeleton changes as it is well known that the cytoskeleton plays an important role in cell motility. In particular, we analysed the pattern of the intermediate filaments of vimentin and of microtubuli in Jurkat cells. The higher cytoplasmic volume and size of these cells, compared to resting lymphocytes, render them more suitable for this type of analysis.
The cells were fixed with paraformaldehyde 30, 420 & 720 s after the onset of µg. After recovery, the cytoskeleton was marked with monoclonal antibodies anti-vimentin and anti-tubulin. The cytofluorographs of the intermediate filaments of vimentin are shown in Fig. 2; the quantitative evaluation of the changes is presented in Fig. 3.
Fig. 2. Cytofluorographs of the intermediate filaments of vimentin of Jurkat cells. Top: ground control; Bottom: flight sample fixed after 30 s in µg.
Fig. 3. Influence of gravity changes on the structure of Vimentin in Jurkat cells. The bars represent the percentage of cells showing either the appearance of large bundles (solid bars) or the formation of protein aggregates together with discontinuities of filamentous network (cross-hatched bars). Standard errors of the means are given.
The morphological analysis reveals that cytoskeleton structural changes are occurring in µg and that they are most evident in vimentin. We must point out that some of the changes were also detected, although to a significantly smaller degree, in the control samples.
The most important difference is given by the intermediate filaments of vimentin which under µg assemble into thick bundles in 20.9±1.7% of the cells, compared to 9.9±1.3% (95% significance of the difference; U(3,3) = 0, alpha = 0.05) in 1 g. This change is observed after only 30 s of µg. The number of cells showing large bundles is slightly but significantly decreased at µg times of 420 s (17.8±0.4%, 95% significance, U(3,3) = 0, alpha = 0.05) and 720 s (18.8±1.6%, 95% significance, U(3,3) = 0, alpha = 0.05).
The other changes observed in µg consist of the formation of aggregates of protein, appearing as fluorescent points in Fig. 2 (bottom) and suggesting depolimerisation processes, as well as of discontinuities of the filamentous network (11.9±1.3% at 30 s, 12.1±2.3% at 420 s and 11.0±1.3% at 720 s, compared to 8.8±1.6% of the ground control. Also, these changes are significant (95% at 30 s and 90% at 420 & 720 s).
A significant difference in the appearance of large bundles has also been observed in the microtubuli network, where 13.2±1.2% of the cells show these changes after 30 s of µg, compared to 5.9±2.8% in the ground control (95% significance, U(3,3) = 0, alpha = 0.05). After 720 s, 11.8±1.5% of the cells show large bundles. Also, this difference is significant (95%, U(3,3) = 0, alpha = 0.05). The other changes are less evident.
During the sounding rocket's boost phase, the cells were exposed to hypergravitational forces. In a control experiment, cells loaded into flight-type syringes were exposed for 2 min to 13 g at 37°C and than fixed. Analysis of the structure of vimentin showed the appearance of large bundles in 8.9±1.2% of the cells, whereas the formation of aggregates was found in 8.0±1.1%. Thus these cells do not exhibit the effects observed in µg. The same is true for the microtubuli network where 7.55±1.2% of the cells exposed to hypergravity showed large bundles. Therefore, the observed changes in the cytoskeletal structure are due to µg and not to the g-stress caused by the launch or a combination of hypergravity and gravitational unloading. This is also supported by the fact that the number of cells displaying the changes are only slightly reduced after 720 s in µg.
The intermediate filaments of vimentin consist of a protein expressed in cells of mesenchimal origin as well as in other types of cells cultured in vitro.14 The proposed function of the intermediate filaments is to provide a structural network, with characteristics typical of each cell type. This network maintains the shape of the cell, the distribution of its organelles and, based on the connections between cytoplasmic membrane and nucleus, mediates the intracellular signal transduction.
Little is known on the function of vimentin in lymphocytes:
Studies carried out on B cells with anti-immunoglobulins
antibodies, have demonstrated that this protein is involved in
the capping of surface immunoglobulins in the region of the
Of particular interest is the observation that in T lymphocytes, the activation by Con A causes an alteration in the distribution of vimentin that appears to be correlated with the phases of the cellular cycle.16 This indicates that the role of vimentin may change during the progression of the cell through its cycle. In addition, cytoskeleton changes, beside generating the movements, may reveal the beginning of the apoptosis which was observed in micrographs of lymphocytes cultivated in an earlier experiment in space²
A recent article 17 discussed the role of gravity in pattern formation of microtubule in solutions of purified tubulin. The pattern was different depending on whether the reaction containers were in the upright or horizontal position. This phenomenon is related to the bifurcations occurring in non- linear out of equilibrium states. As discussed by the authors in a previous paper,18 the transition from 1 g to µg may result in a bifurcation point at which the biological systems drastically changes its behaviour.
The data presented here, together with the authors' previous findings in space,1-5 support the bifurcation theory and the control of pattern formation of cytoskeletal structures by gravity.
Cell motility
The images recorded under µg clearly show that the free-floating non-activated cells were able to display autonomous motion in random directions. Fig. 4A shows the overall displacement of 11 different cells. A detailed analysis (one image every 13 s) shows that the movements were much more complex. The cells often changed direction, moved back and forwards and sometimes crossed the same point several times (Fig. 4B). The average velocity, calculated from the displacement in the 13 s increment, was 8.4±1.2 µm/min, with a range of 0-29.4 µm/min. Also of interest is the observation that the cells in µg were not all round. They very often exhibited longitudinal forms, rotated around their axis and also showed contraction waves similar to those described in the literature for lymphocytes that move in collagen gels under 1 g conditions.19 All 11 cells in the observation field showed motion capability under µg conditions. This is in contradiction to the behaviour of lymphocytes under 1 g conditions, where mostly activated cells only or cells in the presence of a chemoattractant show this capability.20
Fig. 4. Motility and interactions of human lymphocytes in µg. A: overall movement of 11 different cells; B: detailed motion behaviour of four cells (one measuring point each 13 s). The numbers on the axes, in µm, correspond to the position of the cell in the viewing field.
The origin of the movement can be attributed to two major causes. First, changes of the cytoskeleton, in particular the formation of thick bundles of microfilaments, may induce motility of lymphocytes by contraction and elongation of the free-floating cell. Second, the Marangoni convection due to differences in the concentration of components dissolved in the medium may generate the movements of cells resuspended in µg.21, 22Concentration gradients are generated by the metabolism of the cells, which are consuming nutrients (mainly glucose and glutamine) and producing waste (such as lactate and ammonia). Recent data (Cogoli-Greuter, Dall'Aglio, Bedarida and Cogoli, unpublished results) based on laser diffraction studies clearly show that concentration gradients form in cultures of lymphocytes and Jurkat cells.
In a recent experiment performed in the NIZEMI unit during the Space Shuttle's IML-2 mission, the motility and aggregate formation of human lymphocytes during the in vitro activation with Con A was observed in real time.23 The velocity of free cells (cells not attached to an aggregate) in the presence of Con A was found to be in the same range as in the absence of the mitogen: 6.6±1.4 µm/min. Interestingly, the velocity did not change during the 3 days of incubation, whereas in the ground control, the velocity of single cells was significantly lower and decreased with increasing incubation time.
Nevertheless, although the movements and interactions observed under µg in free-floating cells suggest that cell-cell interactions and signal transmission may work as at 1 g, it is important to remark that the cytoskeleton is altered and that, therefore, signal transmission and transduction may be altered as well. Also, the aggregates formed by lymphocytes are smaller and less frequent under µg than at 1 g 2, 23
The experiments described here have contributed to clarifying certain aspects of the in vitro activation of T lymphocytes in microgravity:
This project was conducted with grants from the Italian Space Agency (ASI), the Swiss National Research Foundation (Grant No. 31-25181.88), ETH Zürich, Contraves A.G., Zürich, and ERNO Raumfahrttechnik, Bremen. The authors gratefully acknowledge the assistance of D. Mesland, W. Herfs and D. Frimout of ESA- ESTEC, Noordwijk, of R. Huijser of Fokker Space & Systems, Amsterdam, of H. van Soest and A. Koppen of CCM, Nuenen (NL), of the team of ERNO Raumfahrttechnik, Bremen, in particular D. Grothe and I. Meyer, and of the team of the Swedish Space Corporation at the ESRANGE launch facility in Kiruna, Sweden. The authors also wish to thank A. Lanzavecchia of the Basle Institute of Immunology for providing the Jurkat cells.


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Chlorine Free Pools in Achrain (Bavaria) Upper Bavaria > Bavaria > Germany
Traveling to Achrain? This really is the right spot on the internet to find places to swim in Achrain. Each day you might be not swimming you are losing precious time to improve your skills a be a better swimmer! No much more excuses!. Looking for Chlorine Free Pools in Achrain (Bavaria) (Bavaria)? A lot of of them belong to gyms or sport centers which also offer lessons.
Thanks to some new Chlorine Free Pools in Achrain (Bavaria) (Bavaria) submitted every single day, Swimia.com is increasing day-to-day the number of stored pools. Please don't hesitate to contact us when you find any inaccurate or incomplete information. Please check the nearest Chlorine Free Pools in Achrain (Bavaria) (Bavaria) or Contact us to ADD IT. It is free!
Please, use the contact information provided at every single pool profile.
Nearest Chlorine Free Pools in Achrain, located 4
Holmes Place - Hamburg
Updated on 2017 Aussenalster , Bostelreihe 2 , Hamburg , GERMANY (Postal Code: 22083). Among all the activities offered by the center, for anyone who is interested in swimming you will get pleasure from 25 meters x 10 meters, Non-Chlorine Sanitized, Indoors. If you need to contact this Pool, email address and phone number are both number.
Schwimm- und Sprunghalle im Europa-Sportpark
Paul-Heyse-Straße 26 , Prenzlauer Berg / Friedrichshain , Berlin , GERMANY (Postal Code: 10407). Few days ago a normal user of this center told us they offer Olympic size x 25 meters, 10 lanes, 3 meters depth, 3 meters depth, Non-Chlorine Sanitized, Indoors. Furthermore, they keep in good conditions Olympic size x 25 meters, 10 lanes, 2 meters depth, 2 meters depth, Indoors. For this Swimming Pool, only phone number is number here.
Hallenbad Rheine
Hemelter Straße 38 , Rheine , GERMANY (Postal Code: 48429). Swimmers like you reported 25 meters x 12.5 meters, Salt Water, Non-Chlorine Sanitized, Indoors. Unfortunately we do not have the e-mail address of this Pool, but we can give you the phone number.
Im Neuenheimer Feld 710 , Neuenheim , Heidelberg , GERMANY (Postal Code: 69120). This sports center offers Olympic size x 25 meters, Non-Chlorine Sanitized, Indoors. For this Swimming Pool, only phone number is number here.


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Mencari Pekerja Bebas atau Kerja Inn cotton?
Perlu bantuan untuk Inn cotton? Upah freelancer hari ini! Adakah anda pakar dalam Inn cotton? Gunakan kemahiran Inn cotton anda dan bermula untuk menjana pendapatan dalam talian hari ini! Freelancer adalah pasaran terbesar untuk pekerjaan dalam dunia. Terdapat 17,764 kerja sedang menunggu anda untuk bermula bekerja!Saya Mahu Upah Pekerja Saya Ingin Bekerja
Pekerjaan Inn cotton:
|Harga (MYR)
|Harga (MYR)
|Email Blast for Black Friday Campaign
|Hi! We're looking to hire someone to design an email blast for our retail customers. We'll provide the content, pictures and inspiration. We are a luxury recycled cotton fabric brand that specializes in gift wrap, gift bags, wine bags and accessories. We think this will be a 1 hour project.
|Reka Bentuk Grafik, Rekabentuk Logo, Pemasaran, Pemasaran Pukal, Identiti Korporat
|Nov 19, 2017
|Hari Ini6h 10j
|Holiday Email Blast
|Hi! We are a small luxury recycled cotton brand that specializes in gift wrap, gift bags and accessories. We're looking for an email blast to send for black friday. We'd like one to send to our retail customers and one for our wholesale partners.
|Reka Bentuk Grafik, Pengiklanan, Pemasaran E-mel
|Nov 19, 2017
|Hari Ini6h 9j
|i need a fashion designer
|i have cotton frock manufacturing
|Reka Bentuk Fesyen
|Nov 19, 2017
|Hari Ini6h 7j
|customise work and in wowonder and wonderful themeforest theme and website and wowonder mobile app uploading live total work
|[URL dibuang, sila log masuk untuk melihat] 1. this is wowonder theme of themeforest for social community , i am looking for few customise work after purchasing, i.e. personal details of profile similar to linked inn and few more features 2. readymade mobile app is availble on themeforest of the same thme, chaking and making live the same
|PHP, Reka Bentuk Laman Web, Android, Wordpress, HTML
|Nov 19, 2017
|Hari Ini6h 2j
|Design project
|I need a logo designed. Hi, My name is Aditi. I 'm looking for a logo for my cottage in manali. The name of the cottage is Driftwood Inn Cottage.
|Reka Bentuk Grafik, Rekabentuk Logo
|Nov 18, 2017
|Nov 18, 20175h 17j
|I need a stationer designer
|I make handmade paper from waste office paper, cotton fabric and plant fibres. I am based in Southern Africa and looking for someone to help me design notebooks, boxes, gift bags etc.. with an African theme with international appeal. My target is the tourism market (souvenir notebooks for example) and the events industry (invitations, favours etc...)
|Rekabentuk Alat Tulis
|Nov 18, 2017
|Nov 18, 20175h 8j
|Project for Dreamappsolution -- 2
|inn app purchase and trial features on my project
|PHP, Java, Mobile App Development, iPhone, Android,
|Nov 18, 2017
|Nov 18, 20178h 7j
|Create graphic print designs for our Canvas handbags collection for USA.
|I am launching a new product range consisting handbag, home and gift line of products for both men and women based in USA, Canada, Europe and Australia. It's a eco-friendly product, used natural cotton and vegetable tanned leather only for reinforcements. Products convey the rugged and vintage look and each season will be based upon new theme like travel, adventure, sea beach etc. etc. My com...
|Reka Bentuk Grafik
|Nov 17, 2017
|Nov 17, 20174h 9j
|arrange the details for importers in your country
|hi friend, my name is revat taksande, director & marketing manager in larvin international. we , LARVIN INTERNATIONAL is based in ahmedabad, gujarat, india. we are in to exports of spices, onion and garlic powder, onion and garlic flakes, vegetables, tea, coffee, cotton, organic seeds, salt, clothes (mens, womens kids wear). my focus is not to hire you my friend, we are here to make trade rel...
|Pemasaran Internet, Telepemasaran, Jualan, Pemasaran Pukal, Prospek
|Nov 16, 2017
|Nov 16, 20173h 2j
|Big Cartel Website.
|Inspire By Design is a Printing company that makes screen printed shirts, banners, business cards Flyers Logo design etc. We feel that a simple but very functional site would work well with our line of business. We have a big cartel and we also have a template we have payed for. Below are some of the sites we would like to emulate. [URL dibuang, sila log masuk untuk melihat] - header / drop dow...
|PHP, Reka Bentuk Laman Web
|Nov 14, 2017
|Nov 14, 20171h 10j
|Web development
|Max 1 -2 pages responsive /parallax design with a good minimalist layout for a small Inn that can be customised and expanded later.
|PHP, Reka Bentuk Laman Web, Reka Bentuk Grafik, Pasang Blog
|Nov 14, 2017
|Nov 14, 20171h 3j
|aa0 kabhi haweli pqi t-shirt
|Best t shart 100% cotton with soft wash. This apparel is already pre-shrunk so you wont have to worry about the apparel shrinking after wash. Additionally, we ensure that there is no colour fading on any of our apparel [URL dibuang, sila log masuk untuk melihat] think this one will fit right into your on-trend wardrobe. You think so too? Well, time to get your hands on one!
|Reka Bentuk Grafik, Photoshop, Pengilustrasi, Reka Bentuk Photoshop, Kemeja-T
|Nov 13, 2017
|Nov 13, 20179j 38m
|Design a A-Frame and 8x10 Event Ads
|A-Frame Foam Board Size - 22" X 28" Poster Size 8x10" Banner for Facebook Title of Event: Annual Halloween Brunch Pawty 2018 Host by Teddeeland (logo attached) Date of Event: Sunday, October 7, 2018 Venue: Poplar Springs Inn & Spa 5025 Casanova Road Warrenton, VA 20187 (Pictures attached) Time: 10:00 a.m - 2:00 p.m This is a...
|Reka Bentuk Sepanduk, Reka Bentuk Grafik, Rekabentuk Logo, Photoshop, Reka Bentuk Photoshop
|Nov 10, 2017
|Nov 10, 201720h 11j
|Crop Gross Margin on Excel
|We want to integrate a number of gross margins into one interactive spreadsheet that incorporates a summary sheet of all gross margins at the start, individual crops on separate tabs and a prices sheet at the end. We want to be able to change a price in the price tab and it change all of the prices throughout all of the gross margins and automatically update the Gross margins summary at the start....
|Visual Basic, Pemprosesan Data, Pemasukan Data, Excel, Photoshop
|Nov 9, 2017
|Nov 9, 2017Tamat
|Need assistance to find vendor for T Shirt manufacturer (Description Below) in India
|I am looking for someone from India who can help me in searching a vendor for following product:- 1. Plain solid round neck (Black color) 2. 100% Cotton (Pure quality - Pima Cotton) 3. Mercerized Cotton / very good quality 4. Rebranding (I have to put my tag) I tried India Mart and other places but due to lack of time, I am not able to work on this at the moment. Since I am hardpressed w...
|Reka Bentuk Fesyen, Kemeja-T
|Nov 9, 2017
|Nov 9, 2017Tamat
|product listing Magento 2.2.0
|I'm looking for a way to set up my products so two attributes are linked together or share the same data. Our products can have as many as 60 different color variations and customers don't only look for a color (visible swatch picture) but also the for a color name as there are many slight color difference in our productline we'd like to use visual swatches and a drop down which ...
|PHP, Reka Bentuk Laman Web, Magento, eDagang, HTML
|Nov 8, 2017
|Nov 8, 2017Tamat
|Need Buyer for QC Passed SURPLUS Export Standard Blanket
|- Looking to have buyer for blankets (fresh n QC passed) - Material: Cotton - Technique: Woven - Design: Hexagonal - Available in Twin Sizes (86 "L x 68" W) - Available in Queen Size (90" L x 90" W) - Available in King Size (90" L x 108" W) -Available Quantity: 36 units in each category. -
|Pemasaran, Pemasaran Pukal, Penyumberan Pembeli
|Nov 8, 2017
|Nov 8, 2017Tamat
|Gralak's Brasil
|A roadside inn in Brazil, local dishes, cold hot drinks and local beers
|Rekabentuk Logo
|Nov 7, 2017
|Nov 7, 2017Tamat
|Magento 2.2.0 - Product page - linked attributes - swatches and dropdown.
|I'm looking for a way to set up my products so two attributes are linked together or share the same data. Our products can have as many as 60 different color variations and customers don't only look for a color (visible swatch picture) but also the for a color name as there are many slight color difference in our productline we'd like to use visual swatches and a drop down which ...
|PHP, Javascript, Magento, eDagang, HTML
|Nov 6, 2017
|Nov 6, 2017Tamat
|Søker nordmenn til å bygge helnorsk freelance-team
|Det er eit stort freelancemarked over heile verda, også for tjenester frå nordmenn (oversetting, skriving, markedsføring +++) Derfor ønsker eg å samle mange av desse ressursane til eit team som kan sikre seg ein solid posisjon i dette markedet. På denne måten kan vi sørge for kontinuerlig arbeid i staden for sporadiske oppdrag, og opplevelsen a...
|Nov 5, 2017
|Nov 5, 2017Tamat
Showing 1 to 20 of 2841 entries
Upah Freelancer Inn cotton Terbaik:


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01387 424263 (Sales)
01387 267835 (Aftersales)
Every Kia driver is unique, which is why we've designed a wide range of additional features and car accessories to make your Kia truly your own.
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WWIIBest Gamemode in WW2?Posted:
Status: Offline
Joined: Nov 13, 20161 Year Member
Posts: 71
Reputation Power: 2
|What's the best gamemode to rank up in WW2? Just curious to know what everyone likes playing
#2. Posted:
- Gold Member
Status: Offline
Joined: Jul 14, 20125 Year Member
Posts: 881
Reputation Power: 44
Motto: Don't Miss, Don't Lose |
|Love playing some Hardpoint or Domination.
- 0useful
- 0not useful
#3. Posted:
Status: Offline
Joined: Jun 24, 2017
Posts: 83
Reputation Power: 8
|To rank up, probably Capture The Flag, hence why all the booster play that. Personally, TDM and Search will always be my favorites though.
- 0useful
- 0not useful
#4. Posted:
- Gold Member
Status: Offline
Joined: Jun 27, 20098 Year Member
Posts: 1,488
Reputation Power: 64
|War and Kill Confirmed are the best modes I think for gaining XP.
- 0useful
- 0not useful
#5. Posted:
- Tutorial King
Status: Offline
Joined: Apr 24, 20152 Year Member
Posts: 9,669
Reputation Power: 1353
|Im loving playing a bit of Hardcore Domination or Core Free-For-All.
- 0useful
- 0not useful
#6. Posted:
Status: Offline
Joined: Feb 20, 20134 Year Member
Posts: 2,237
Reputation Power: 113
War, Kill Confirmed and Hardpoint were the best. But now the XP rate has been scaled back as it was "Double XP" since launch.
I think you have to experiment because I believe War will take a massive hit.
- 0useful
- 0not useful
#7. Posted:
- Christmas!
Status: Offline
Joined: Feb 20, 20152 Year Member
Posts: 4,050
Reputation Power: 222
Motto: | Life is just a game |
|Hardcore TDM or TDM or War
- 0useful
- 0not useful
#8. Posted:
- 1K Rainmaker
Status: Online
Joined: Mar 25, 20161 Year Member
Posts: 2,392
Reputation Power: 138
Motto: S/O dah for the gold | youtube.com/c/imthehomiematt
|i like TDM
- 0useful
- 0not useful
#9. Posted:
- Gold Member
Status: Offline
Joined: Feb 12, 20116 Year Member
Posts: 763
Reputation Power: 53
Motto: WWII
|For me, I enjoy playing either Domination or Search and Destroy. I find that if you're playing in a party of 6 players, you'll rank up quick enough.
- 0useful
- 0not useful
#10. Posted:
Status: Offline
Joined: May 26, 20116 Year Member
Posts: 1,788
Reputation Power: 206
Motto: Top Pages Singles And Top Page TL l USS Texas SnD expert l ONLY GT - Tight l 400k+ on Twitter l Shoe Botter l Sneakerhead/SneakerEnthusiast l ONLY DEAL ONSITE l 87% OF 2015, 2016 And 2017 Members Need Helmet
|Hardpoint/SnD/CTF but Hardpoint is practically playing Cod champs Every lobby
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Users browsing this topic: None


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Five for Fighting – 100 Years - Lyrics (HQ)| play
Jan 14, 2013 at 11:15 pm via YouTube | AMAZON | ITUNES
NickSteel gave 4 props on Jan 14 at 4:50 pm


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Three people are being held in connection with the theft of paintings worth at least 100 million dollars from a Paris gallery last year, but the works are still missing, a legal official said Saturday. The three, a woman suspected of taking part in the theft and two people suspected of handling stolen goods, were arrested and charged over the robbery of the five paintings, by Henri Matisse, Pablo Picasso, Georges Braque, Ferdinand Leger and Amedeo Modigliani, and placed in custody on September 16, the official said. A lone burglar sheared off a gate padlock and broke a window to get into the city-run Musee d'Art Moderne in the brazen operation during the night of May 19 last year. The paintings were found to be missing just as the museum, a major tourist attraction near the Eiffel Tower, was about to open. Paris mayor Bertrand Delanoe later said one of the museum's alarms had been "partly malfunctioning" since the end of March, and that it was still awaiting repair when the thieves struck. The stolen Picasso alone -- the cubist "Dove with Green Peas," which the Spanish artist created in 1912 -- is worth some 25 million euros, according to the mayor's deputy for culture, Christophe Girard. The others were French contemporary Matisse's "Pastoral" from 1905, Braque's "Olive Tree near Estaque", Modigliani's "Woman with a Fan" and Leger's "Still Life with Candlestick". City hall put the total value of the haul at 100 million euros ($140 million), but some experts said they were worth twice that, while admitting they were totally unsaleable openly. Girard said the thief managed to slip past three nightwatchmen on duty. "To get into the museum so fast by disassembling a window, choose five specific works and then slip out unnoticed by the guards, that is quite impressive," he said.
GMT 06:27 2017 Tuesday ,21 NovemberRegional journalists urged to promote local cultural, heritage
GMT 06:23 2017 Monday ,20 NovemberProceeds from Sharjah Children Biennial's artworks to aid refugees
GMT 04:52 2017 Monday ,20 NovemberArt performance inspired by Abu Dhabi's unique urban fabric launched
GMT 04:49 2017 Monday ,20 NovemberNational Bonds launches nationwide campaign to encourage personal saving
GMT 04:40 2017 Monday ,20 NovemberEgypt praises Sharjah Ruler's initiative for delivering 354 Egyptian artifacts
GMT 04:27 2017 Monday ,20 NovemberAbu Dhabi Art's 9th edition opens this week
GMT 13:05 2017 Sunday ,19 NovemberFVP Briefed for holding Conference of Minister of Cultures in Islamic Countries
GMT 06:26 2017 Sunday ,19 NovemberEmirates Heritage Club launches programme on mangroves cultivation


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By WeChat
This app is only available on the App Store for iOS devices.
WeChat is a messaging and calling app that allows you to easily connect with family & friends across countries. It's the all-in-one communications app for text (SMS/MMS), voice and video calls, Moments, photo sharing, and games.
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• MEET NEW FRIENDS: Use "Friend Radar", "People Nearby" and "Shake" to meet others.
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What's New in Version 6.5.22
In this update
- Compatibility with iPhone X
- Filter your chat history for payment transactions
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Latest updates
- Notes in Favorites can be formatted with bulleted and numbered lists, and notes can be set to Sticky on Top of the chat screen
- Generate your own tipping code and present to others to receive tips from other users (China-only)
- Improved chat history search experience
- A whole new design for iPad including an dual column layout
Customer Reviews
People nearby & shake
I always got issue with the people nearby and shake.. i make a new account and then my people nearby is not even working.. i can other people but people couldnt see me.. i know this because i use my other account to check my new account on people nearby.. same goes to shake.. at first it is working but few days like that i would not work anymore
Got blocked for no reason at all
I got this because a friend of my sons parent uses it so when they play this is how we kept in contact. Have done nothing but chat with her. A few days into having it my account login was blocked saying I violated terms. ??? Well screw you too then WhatsApp, texting from now on it is lol
Account locked / protected and have attempted to unlock via instructions provided with three 3 different wechat friend who all get an error. What crap software. No other mechanism to contact wechat for help, what a joke. App is completely unusable now simple because I didn't use it for several months!!!!!!!!
- Free
- Category: Social Networking
- Updated: Nov 15, 2017
- Version: 6.5.22
- Size: 228 MB
- Apple Watch: Yes
- Languages: English, Arabic, Chinese (Hong Kong), French, German, Hebrew, Hindi, Indonesian, Italian, Japanese, Korean, Malay, Polish, Portuguese, Russian, Simplified Chinese, Spanish, Thai, Traditional Chinese, Turkish, Vietnamese
- Seller: Tencent Technology (Shenzhen) Company Limited
- © Tencent Inc.
Compatibility: Requires iOS 8.0 or later. Compatible with iPhone, iPad, and iPod touch.
Top In-App Purchases
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- elephant DEE$0.99
- Doraemon$0.99


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You will receive 12 Black Alligator Clips with teeth. ::Size:: approximately 3 Inch (75mm) Length ::Material:: Metal Hair Clip , Nickel Plated Steel , Rust Resistant Finish Strong yet LightWeight , Durable Coil Spring
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