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High performance liquid chromatography (HPLC) coupled with a Fraction Collector was employed to isolate Maillard reaction products (MRPs) formed in model systems comprising of asparagine and monosaccharides in the 60–180°C range. The primary MRP which is detected at 60°C is important for Acrylamide content and color/aroma development in foods and also in the field of food biotechnology for controlling the extent of the Maillard reaction with temperature. The discrete fractions of the reaction products were reacted with Hemoglobin (Hb) and Myoglobin (Mb) at physiological conditions and the reaction adducts were monitored by UV-vis and Attenuated Total Reflection-Fourier transform infrared (FTIR) spectrophotometry. The UV-vis kinetic profiles revealed the formation of a Soret transition characteristic of a low-spin six-coordinated species and the ATR-FTIR spectrum of the Hb-MRP and Mb-MRP fractions showed modifications in the protein Amide I and II vibrations. The UV-vis and the FTIR spectra of the Hb-MRPs indicate that the six-coordinated species is a hemichrome in which the distal E7 Histidine is coordinated to the heme Fe and blocks irreversibly the ligand binding site. Although the Mb-MRPs complex is a six-coordinated species, the 1608 cm-1 FTIR band characteristic of a hemichrome was not observed.
Citation: Ioannou A, Varotsis C (2017) Modifications of hemoglobin and myoglobin by Maillard reaction products (MRPs). PLoS ONE12(11): e0188095. https://doi.org/10.1371/journal.pone.0188095
Editor: Ram Nagaraj, University of Colorado Denver School of Medicine, UNITED STATES
Received: July 24, 2017; Accepted: October 31, 2017; Published: November 14, 2017
Copyright: © 2017 Ioannou, Varotsis. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper.
Funding: Financial support by the European Regional Development Fund and the Republic of Cyprus through the Research Promotion Foundation (Grant YGEIA/TROFH/0311(BIE)/04) is gratefully acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The glycation process as illustrated by Maillard reactions and its health consequences have gained considerable attention and have been studied extensively in recent years [1–9]. Glycation processes cause the pathology in diabetes principally by the non-enzymatic modification of proteins by glucose and other products of glucose metabolism. The glycation reaction's kinetics are heightened by elevated and prolonged exposure to glucose and other glycated species, which in turn leads to the chronic health problems. The classical example is that of the post-translational modification of hemoglobin occurring from the covalently bound intermediate arising from the interaction of the electrophilic glucose groups with the nucleophilic primary amino-groups of protein amino-acid residues. Hemoglobin advanced glycation end products (Hb-AGEs) are formed when the initial Schiff bases typically undergoes an Amadori rearrangement resulting in the formation a fructosamin (ketosamin) [10] (Fig 1).
Glycated hemoglobin (Hb) is an effective index of long-term blood glucose level and has been widely used in the diagnosis of diabetes mellitus. Glycated Hb comprises HbA1 (Amadori product) and other Hb variants and adducts [11]. HbA1c which is the major component of HbA1 is formed by a non-enzymatic irreversible process in which the aldehyde group of glucose combines with the amino-terminal valine of the β-chain of Hb [12]. More broadly, the terms glycated hemoglobin or glycohemoglobin (GHb, GlcHb) refer to the full range of glycated hemoglobins, including those containing glycated valine and/or lysine residues. The major hemoglobin glycation sites in vitro and in vivo include β-Lys-66 and β-Lys-120 [13]. The presence of an AGE-modification alters or destroys enzymatic activity, one example being methylglyoxal-modified serum albumin [14].
Hemichromes are formed when hemoglobin undergoes conformational changes resulting in the formation of a six-coordinated Fe3+ low-spin His-Fe-His species [15]. Hemoglobin A in humans can form hemichromes even under physiological conditions as a result of pH and temperature alterations, and ιn the autoxidation of oxyHb [15]. Hemichromes are mainly produced by partially denatured hemoglobin and thought to be precursors of Hb denaturation processes, such as unfolding, precipitation, and heme dissociation. In tetrameric α2β2 Hb it is always thought that oxidized forms adopt either aquo-met or hydroxy-met states, according on the pH of their medium. However, it was recently shown that alternate forms like bis-histidyl hemichrome states could be compatible with folded structures [16]. The crystal structure of Hb in which a partial hemichrome was formed has been reported [17].
In the Maillard reactions a cascade of events each with a discrete role can affect the product composition and generate distinct colors and aromas. One of the factors with instructive significance is temperature and for this reason we have investigate the reaction in the 60–180°C range. Many of the studies on protein glycation deal with identification of the glycated protein sites by LC-MS. In this work, we have monitored the reactions of the isolated MRPs products which are originated from the reactions of 1) asparagine with glucose and 2) asparagine with fructose and were separated and isolated by an HPLC component system coupled to a fraction collector in the 60–180°C range and found that the primary reaction product is formed at 60°C. Because the Maillard reaction is a cascade of consecutive and parallel reaction steps, it is important for the food biotechnology industry to be able to control the extent of the Maillard reaction with temperature. All reaction products were reacted with Hb and Mb. Our results demonstrate the formation of hemichromes in the reactions of the individual MRPs products originated from the Asn/Gluc and Asn/Fruc reactions with Hb, as indicated by 1) the UV-vis data for the formation of a six-coordinate species and 2) the observation of the 1608 cm-1 protein Hb FTIR band. On the other hand, although Mb has a number of ε-amino groups which could be glycated as similarly occurring in Hb and the UV-vis difference spectra show peaks/troughs at 425/407 nm with a zero-crossing at 420 nm which is characteristic of a six-coordinated species, the 1608 cm-1 FTIR band was not observed in none of the Mb-MRPs. Of note is a 6 cm-1 downshift in both the Amide I and II bands that demonstrate conformational changes in all of the Mb-MRPs.
Materials and methods
Sample preparation
Equimolar solutions of glucose or fructose and asparagine (0.2 M) were prepared in phosphate buffer (50 mM) and the pH was adjusted to 8.0. Samples (10 ml) were heated in closed screw-capped tubes at 60, 80, 100 and 180°C in a heating oven (Memmert, Germany) for 2 hours. For HPLC analysis the samples were diluted hundred-fold.
Spectroscopic monitoring of hemoglobin with added MRPs
Hemoglobin and myoglobin (1 mM) were dissolved in 100 mM potassium phosphate buffer (pH 8.0) at 25°C. The native protein solutions (oxidized form) were used as the blank sample in the spectrophotometric cell. Hemoglobin and myoglobin were diluted hundred-fold and immediately mixed with each individual LC fraction (2:1 v/v) from the reaction between asparagine and glucose or fructose. Immediately upon mixing the protein and fraction solutions, a time cycle program on the UV-vis spectrophotometer software was commenced. The time cycle program allows for the automated collection of sample measurements at predetermined time periods.
HPLC-Fraction collector analysis
The customized HPLC experimental setup consisted of a Varian 218 Prepstar Solvent Delivery Module, an Agilent Manual FL-Injection Valve, an Agilent 1260 Infinity Variable Wavelength Detector (VWD) and an Agilent 440 LC Fraction Collector. Water at a flow rate of 0.5 ml/min was used isocratically as the mobile phase at room temperature. A 20 μL aliquot of sample was injected. Maillard reaction products (MPRs) were detected at 200 nm. An automated single probe Agilent Technologies 440 fraction collector was coupled to the chromatographic system. The instrumental setup and the separation of individual fractions were described in detail earlier [18]. HPLC analysis was performed on a 4.6 x 250 mm, 5 μm particle size, Zorbax SB-Aq analytical column (Agilent Technologies). Maillard reaction products (MPRs) were analyzed under aqueous conditions using a previously reported method [19]. The fraction collector employed the Agilent OpenLAB Software real-time peak detection algorithms to achieve accurate and reproducible chromatographic detection of fractions. Individual resolved elements from a multi-component sample were collected by means of a time-slice method. During the chromatographic run the fraction collector allows for collection at defined time intervals. Fractions were collected in time slices thus creating a time frame window for each chromatographic peak. This was achieved by means of a diverter valve which is switched from the waste to the collect position. The time frames for each eluting peak and the system delay time were incorporated to a time program table created by the Agilent OpenLAB Software. Mobile phase during equilibration between chromatographic runs was sent to the waste position since the valve was switched back to this position after the finish of each run. Pooling of identical fractions between runs was also performed to enhance FTIR detection." The instrumental setup and the separation of individual fractions were described in detail earlier [18]. HPLC analysis was performed on a 4.6 x 250 mm, 5 μm particle size, Zorbax SB-Aq analytical column (Agilent Technologies). Maillard reaction products (MPRs) were analyzed under aqueous conditions using a previously reported method [19].
UV-vis spectrophotometry
Hemoglobin and myoglobin were dissolved in 100 mM potassium phosphate buffer (pH 8.0) at 25°C. The native protein solutions (oxidized form) were used as the blank sample in the spectrophotometric cell. Once the protein solutions were used to perform the blank measurement, the fraction solutions were added to the protein solution in the cell and the first sample measurement at time 0 minutes was performed by means of a time cycle program on the UV-vis Lambda 25 Perkin Elmer spectrophotometer software. Difference spectra (reacted Hb or Mb with MRPs minus oxidized Hb or Mb) were collected at time intervals of 5 minutes apart for a time period of 120 minutes in the wavelength range 200–500 nm. The time cycle program allows for the automated collection of sample measurements at predetermined time periods.
ATR-FTIR spectrophotometry
A horizontal ATR (HATR) accessory (Pike Technologies, Inc, Madison, USA) was employed fitting a Germanium ATR plate with ten internal reflections. The Horizontal ATR employs a pair of transfer optics to direct the infrared beam of the spectrometer to one end of the IR transmitting ATR crystal. A similar pair of optics directs the beam emitted from the other end of the ATR crystal to the spectrometer detector. A Tensor 27 FTIR spectrometer (Bruker, Karlsruhe, Germany) equipped with a deuterated triglycine sulfate (DTGS) detector was used for spectral acquisition. Spectra were collected in the range 1,800–800 cm−1 with 4 cm−1 resolution and 100 co-added scans each. A background spectrum was collected before each sample measurement. The software package OPUS 7.0/IR (Bruker) was used to acquire and process the FTIR spectra.
Results and discussion
Isolation and characterization of MRPs by HPLC-Fraction collector
Glycoconjugates, such as N-glycosides and related compounds are recognized as key Maillard reaction intermediates [20]. When asparagine and glucose (Fig 2A and 2B) or fructose (Fig 2C and 2D) reacts in a 60–180°C range it is evident from the HPLC chromatograms that there is elution of several individual products in the reaction mixture (Fig 2). These are even formed at lower temperatures but their yield is significantly increased at higher temperatures. Maillard reaction products (MRPs) were eluted on the Zorbax column which can retain a high selectivity for polar molecules in aqueous phases [19]. Fraction 1 was a combined fraction encompassing a major signal at Rt = 5.7 min corresponding to the excess unreacted asparagine arising from the heated reaction mixture [18]. Fractions 2–5 corresponding to discrete MRPs were collected separately. Fractions 2, 3 and 5 have been characterized to originate from the Schiff base, the Amadori product and acrylamide, respectively [18].
A 20 μL aliquot of sample was injected and the wavelength used for detection was 200 nm. Triplicate samples were manually injected in the chromatographic system. Panel A: Fraction 2 from the reaction of asparagine with glucose. Panel B: Fractions 3–5 collected as discrete MRPs in the reaction of asparagine with glucose. Panel C: Fraction 2 from the reaction of asparagine with fructose. Panel D: Fractions 3–5 collected as discrete MRPs in the reaction of asparagine with fructose.
All fractions displayed characteristic absorbance bands around 294 nm [18] which was previously reported as a generic marker band of Maillard reaction intermediate products [21–23]. These compounds relate to the glycation process as being aldehydes and small molecule ketones arising from the glycosylation of the asparagine molecule. UV absorbance in the range of 270–300 nm is characteristic for molecules containing carbonyl groups (n->π* transitions).
Modifications of hemoglobin and myoglobin by MRPs
Fig 3A depicts the time evolution of the difference absorption spectrum of the reaction product of Hb which is formed upon the addition of the Schiff base LC fraction 2 from the reaction of asparagine and glucose at pH 8 minus oxidized Hb. The difference spectrum shows maxima at 423 nm and minima at 406 nm. The 423 nm transition is attributed to the formation of a six-coordinate species. It has been shown that the selection of a wavelength close to 423 nm is possible a precise measurement for the concentration of glycated hemoglobin [24]. Similar observations have been reported where the spectral difference between hemoglobin and hemichrome was monitored by the difference spectrum with a minimum at 405 nm and a maximum at 423 nm in a fatty acid-hemoglobin system [25]. The UV-vis difference spectra of Hemoglobin with added LC fraction 2 of the reaction mixture of asparagine and fructose, show a 426 nm band that resembles that at 423 nm shown in panel A and an additional transition at 402 nm (Fig 3B). Obviously, the Hb-MRPs originated from the Asn/Glu and Asn/Fruc reactions differ significantly. On the other hand, the analogous Mb-MRPs show strong similarities as indicated by the observation of a peak/trough at 425/410 nm, in both the reactions with the isolated compounds from the Asn/Glu and Asn/Fruc reactions (Fig 3C and 3D).
In panels A (LC fraction 2 from the reaction of Asn with Gluc) and B (LC fraction 2 from the reaction of Asn with Fruc) are the spectra of Hb-MRP adducts formed in the reaction with the LC fraction 2 and in panel C and D are the analogous spectra of Mb. Time course: 0-120mins; Spectra displayed every 30 minutes).
Fig 4A–4D shows the FTIR spectra of the Hb-adducts at pH 8.0 formed from the reactions of Hb with the compounds from the LC Fractions 2–5. The LC fractions were also subjected to ATR-FTIR analysis. However, the LC fractions did not show any FTIR absorption bands due to their low concentration which is much lower than the detection limit of our current ATR-FTIR experimental setup. Therefore, there is no interference from the LC fractions in the ATR-FTIR spectra. In Fig 4A are the FTIR spectra of the Hb-adducts formed from the reactions of Hb with the compounds from the LC fractions 2–5 from the reaction of Asparagine with Glucose and in Fig 4B those from the reactions of LC fractions 2–5 of Asparagine with Fructose. Fig 4C shows the second derivative spectra of the spectra shown in Fig 4A and in Fig 4D are the second derivative spectra of the spectra shown in Fig 4B. Amides I and II are the major bands in the IR spectrum of a protein. Amide I absorption originates from the C = O stretching vibration (70–85%) of the amide group (coupled to in-phase bending of the N–H bond and stretching of the C–N bond), which gives rise to IR band(s) in the region between ~1600 and 1700 cm–1 [25–28]. Amide II originates from the N–H bending (40–60%) and C–N stretching vibrations (18–40%) [26–29]. From the ATR-FTIR spectra, the ratio of the Amide I to Amide II is slightly lower in the Hb-LC Fractions spectra compared to the initial Hb (Fig 4A and 4B) suggesting conformational changes. Another evident feature from the ATR-FTIR spectra is the observation of a new band at 1608 cm-1 in all spectra of the Hb-adducts. We attribute the appearance of the new 1608 cm-1 band to the formation of strong beta-sheet structures [30]. In a recent study probing the α-helix to β-sheet transition in fibrin, a band at 1612–1614 cm-1 was assigned as a marker band of nascent inter-chain β-sheets, consistent with protein aggregation [31]. An additional spectral feature is the increase in intensity of the 1396 cm-1 Hb band which corresponds to the symmetric C = O stretching vibration of COO- groups. This absorption band is related to aspartic acid residues which function as important subunit contact sites. The Tyrα42⋯Aspβ99 H-bond is in the "switch" region of the α1β2 interface [32] involving the interface residues between the αC helix and the βFG corner experience large dislocations during R to T transition. An equally important hydrogen bonded pair is the Trpβ37⋯Aspα94 which comprises of the "hinge" region, in which the R–T shift is restricted to a change in orientation [32]. The above mentioned feature is particularly evident and it seems to be coupled to the appearance of the 1608 cm-1 band in the Hb-LC Fractions 2–4. Fraction 5 forms a very low yield compared to fractions 2–4 of the newly formed 1608 cm-1 band and the 1396 cm-1 band does not exhibit any intensity change. Similar results are obtained in the reactions of Hb with the reactions products of Asn/ Fruc (LC fractions 2–5) shown in Fig 4B. The second derivative spectra of the Hb-adducts formed from the reactions of both the Asn/Gluc and Asn/Fruc reactions shown in Fig 4C and 4D, respectively, show that the amide I vibration remains constant and small frequency changes in the amide II vibration. The presence of the 1608 cm-1 band is evident in both cases.
Panel A. Fractions 2–5 are from the reaction of asparagine with glucose. Panel B. Fractions 2–5 are from the reaction of asparagine with fructose. Panel C. Second derivative spectra of those presented in panel A. Panel D. Second derivative spectra of those presented in panel B.
In the case of Mb-LC Fractions spectra (Fig 5A and 5B), we do not observe the presence of any new bands but it is evident that there is broadening in both Amide I and Amide II FTIR bands. The broadening of the Amide I band may result from the formation of a more β-sheet-like structure since at the tails of the Amide I band lie the β-sheet band subcomponents [30]. Likewise, the minimum between Amide I and Amide II shows significant changes, and more evidently, there is also loss in the intensity of the Amide II band. This is confirmed by the second derivative spectra that reveal also a downshift of 6 cm-1 in the Amide II region (Fig 5C and 5D). This observation suggests that there is also modification of the protein Amide II band. There is also a similar downshift of 5 cm-1 in the Amide I frequency. Amide I and II frequencies are often affected by the strength of hydrogen bonds involving amide C = O and N-H groups. In contrast with hemoglobin, there is also no significant change in the 1396 cm-1 band. This may relate to the fact that there are no subunit contact sites in myoglobin and therefore no alteration in the subunit organization of the protein.
Panel A. Fractions 2–5 are from the reaction of asparagine with glucose. Panel B. Fractions 2–5 are from the reaction of asparagine with fructose. Panel C. Second derivative spectra of those presented in panel A. Panel D. Second derivative spectra of those presented in panel B.
The different behavior of myoglobin versus hemoglobin glycation is interesting to note since it was previously highlighted by a comparative study on the structural stability of myoglobin and glycomyoglobin [33]. The molecular dynamics simulation study revealed an increased stability in the glycomyoglobin molecule as a consequence of increased contacts with water molecules. Moreover, another study has shown that glycation of apomyoglobin with glucose would not effect in fibril formation [34]. This stresses out the importance of the heme component in the whole glycation process. A recent molecular dynamics simulation investigation has pointed out that glucose molecules can interact with heme via the two propionate groups and aspartic and glutamic acid residues by hydrogen bonds, as well as with surrounding water molecules [35].
It was previously suggested that the coordination of distal histidine to the iron produces a scissoring motion in helices E and F which results in modifications in the tertiary structure of the tetramer, mainly in the αβ interface [36]. Raman spectroscopy studies have indicated that this scissor-like motion of helices E and F is critical in the transition from the R to the T state [37–38]. An interesting fact is that in models with mammalian (horse) and Antarctic fish hemoglobins is that the EF fragment modifications are related to a heme sliding motion that exposes the heme to a more solvent-exposed position [16–17]. This results in a shift of the heme group and a narrowing of the heme pocket as the E- and F-helices move toward each other. It is worth mentioning that these movements did not occur in a mutant myoglobin variant examining the function of the distal His [39].
Effector molecules can bind to liganded Hb and this process provides insight into Hb allosteric transition [40]. Previous studies have examined the interaction of polyphenols with serum proteins [41–42] and demonstrated that binding of these molecules to hemoglobin indicate that hydrogen-bonded interactions provide a means of transport of these substances in the blood. The results of protein glycation often vary according to the nature of the modified protein and also the type of carbohydrate molecule. Even though glucose plays the primary role in the formation of glycation products, other monosaccharides have been shown to be sufficient and even act more effectively than glucose to produce glycated variants. Among these, fructose has been shown to perform the glycation process at a much faster rate [43]. Here, we have described the effect of glucose and fructose derivatives and have shown that these also mediate structural alterations on hemoglobin and myoglobin.
There seems to be a link between hemichrome formation and loss of protein functionality [44–45]. Certain reactive molecules, such as glyoxal and methylglyoxal (MG) have been shown to react with proteins to form advanced glycation end products (AGEs) following Maillard-like reaction [46]. Methylglyoxal modification enhanced the structural stability of hemoglobin but it lowered its iron-mediated oxidation reactions [47]. We suggest that tetrameric Hbs form a partial hemichrome state upon addition of MRP compounds as observed in the present spectroscopic investigations. On the same line, these Maillard reaction species act as external ligands binding to an external protein site triggering the formation of six-coordinated bis-histidyl hemichrome species. Bis-histidyl adducts seem to form not only in hemoglobin but also in a monomeric protein such as myoglobin. We have observed these effects in near physiological conditions and these events were not induced in any way by changing experimental conditions to extremes (e.g. temperature, pH, etc.). The heterogeneous and diverse nature of AGE structures often makes the application of a universal biomarker method difficult since these compounds may be present as mixtures in human serum. When examining analytical methods, the effort is to optimize quantitative methods for AGE quantification, such as HbA1c, but not for qualitative analysis. Further investigations could include more detailed studies on exact binding sites and spectroscopic determination of other food-derived compounds with hemoglobin and myoglobin.
Financial support by the European Regional Development Fund and the Republic of Cyprus through the Research Promotion Foundation (Grant YGEIA/TROFH/0311(BIE)/04) is gratefully acknowledged. The authors would like to thank Dr. Antonis Nicolaides for technical assistance.
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- 43. Schalkwijk CG, Stehouwer CD, van Hinsbergh VW. Fructose-mediated non-enzymatic glycation: sweet coupling or bad modification. Diabetes Metab Res Rev. 2004 Sep 1;20(5):369–382. pmid:15343583
- 44. Banerjee S, Maity S, Chakraborti AS. Methylglyoxal-induced modification causes aggregation of myoglobin. Spectrochim. Acta Part A: Mol Biomol Spectrosc. 2016 Feb 15;155:1–10.
- 45. Sen S, Bose T, Roy A, Chakraborti AS. Effect of non-enzymatic glycation on esterase activities of hemoglobin and myoglobin. Mol Cell Biochem. 2007 Jul 1;301(1–2):251–257. pmid:17549609
- 46. Banerjee S, Chakraborti AS. Structural alterations of hemoglobin and myoglobin by glyoxal: a comparative study. Int J Biol Macromolec. 2014 May 31;66:311–318.
- 47. Banerjee S, Chakraborti AS. Methylglyoxal modification enhances the stability of hemoglobin and lowers its iron-mediated oxidation reactions: An in vitro study. Int J Biol Macromolec. 2017 Feb 28;95:1159–1168.


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5 bedroom detached house overlooking beautiful countryside. Padbury is a small rural village only 3 miles from historic Buckingham , 14 miles from Aylesbury, a 20 minute drive from Milton Keynes and approximately 10 miles from Silverstone. Double bedroom, single also available (see separate listing), only rented together to same party. Private bathroom. Television room(not guaranteed)
Tea and coffee making facilities in the bedroom and croissants, cereal and toast for breakfast.
The space
Large 5 bedroom house. Single and double room both with wardrobe, chest of drawers and desk.
Separate television room available dependent on booking date.
Guest access
Guests will have use of their bedroom and bathroom plus a snug with television and sky TV - although this cannot be guaranteed for every booking.
Interaction with guests
I will be around when you are here for anything I can help with.
Other things to note
South facing, views over open countryside. The house is noted as 'of interest' in the Conservation Area Plan.

subset: commoncrawl


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It will be better for you to stain concrete patio surface in your house to get new look and to protect the concrete from the damage properly. You can paint your concrete patio by yourself...Read More →
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love you to death: a year of domestic violence (potential triggers)(21 Posts)
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text: tag:blogger.com,1999:blog-82542599847551939812017-11-21T15:43:49.374-08:00Naomi's Organic Farm SupplyNaomi firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-78687610220233943992017-11-21T15:43:00.000-08:002017-11-21T15:43:49.383-08:00Save 25% during Little Boxes 11/24-26!We'll be closed on Thursday, 11/23 for cozy time with our friends and family. Back open normal hours Friday onwards. Friday, Saturday and Sunday 11/24-26, we are participating in Little Boxes! Download their app on your phone and SAVE 25% off these gift items in our shop: Books Equal Exchange Coffee, Tea and Chocolate Flower Bulbs Indoor Houseplants Seed packets from Adaptive, Native Ideas, Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-82127735157975501902017-11-15T14:37:00.002-08:002017-11-15T14:37:48.873-08:00Swimming in Houseplants + 11/19 Pullet Day The rain is settling in and the clouds are becoming very familiar friends again. As outdoor planting winds down, "gardening" inside gets more exciting. We've got oodles of houseplants of all shapes and sizes to make your indoor spaces lush and cozy. (Nik, Naomi and Carissa, being super serious with incoming plants...) Our next Pullet Day is Sunday 11/19 Pullet Day with teenage hens this Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-56760181380619929982017-11-03T14:02:00.006-07:002017-11-03T14:03:54.006-07:00November Pullet Day on Daylight SavingsPullet Day with teenage hens this Sunday, November 5th at 10am. *Remember daylight falls back this day! Meet in the lot behind our building / south side. Breeds this week include: Silver Laced Wyandottes, Rhode Island Reds, Barred Rocks, Black Australorps, Red Sexlinks, Black Sexlinks, a few Buff Orpingtons and assorted adorable Bantam hens. If you'd like duckings, please let us know by 6pm onNaomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-29195893855307303852017-10-28T10:11:00.001-07:002017-10-28T12:09:38.021-07:00Spooktacular 30% Sale! on all Outdoor PlantsOctober 28th through Sunday, November 5th! Kicking into fall with 30% off all Outdoor Plants!! Includes fruit, natives, flowers, shrubs, veggies and herbs, shade lovers, ground covers and conifers. Through the first day of daylight savings, Sunday, November 5th. It's lovely weather to tuck in goodies for spring. Pumpkins still in stock + we've got a beautiful array of flower bulbs, seed garlic, Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-80306852827599777722017-10-21T14:49:00.001-07:002017-10-21T14:52:17.762-07:00Pullet Day!Pullet Day with teenage hens this Sunday, October 22nd at 10am in the lot behind our building / south side. Breeds this week include: Barred Rocks, Black Australorps, White Leghorns, Red Sexlinks, Black Sexlinks, assorted Bantams. If you'd like duckings, please let us know by 6pm on Saturday 10/21 or you can make arrangements with us for the Pullet Day in two weeks.🐓🍂🎃 More details here.Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-8969905670091167912017-10-14T15:52:00.004-07:002017-10-14T15:52:39.980-07:00Autumn Goodies! Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-80008772490381731432017-09-03T12:32:00.001-07:002017-09-03T12:32:13.236-07:00September Bunny SpaBecause sometimes you just need to be snuggled during your manipedi. ❤ Bunny Spa today from 12-3pm at our shop & first Sunday of every month! All donation proceeds benefit @rabbitadvocates rescue, vet and fostering care. Pawdicures, grooming, scent gland cleaning, mat removal and more by their great volunteer team!🐰 Cinnabun loves the Marvins!! #seportland #cinnabunanddash #rabbitadvocates Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-37703156422555119352017-08-19T12:14:00.002-07:002017-08-26T18:51:32.938-07:00Garlic, gorgeous plants + Closed on 8/21 for Solar EclipseDue to unknown traffic situations that day, we will be closed to keep our team safe. Please protect your eyes, enjoy it and take care. We are brimming with gorgeous pollinator, hummingbird and butterfly friendly plants for late summer and fall planting. Loads of veggie and herb starts, along with many other perennials, native plants and ground covers. This milder weather is lovely for planting. Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-61968459299290989122017-07-03T11:45:00.001-07:002017-07-03T11:45:59.094-07:00Happy 4th! We're open normal hours Monday & Tuesday Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-84958868262720360312017-06-17T10:56:00.001-07:002017-06-18T09:47:05.049-07:00Annual Pride Sale!It's our annual 🌈PRIDE🌈 sale! Sat-Tues = 20% off Fruit Plants, Annual Flowers & Veg, Seed Packets and Books, plus discounts 50% or greater on special plants, pottery and other goods as marked. 📷 of Farmer Katie, always dreamin' of plants. 💕 @foxandbearurbanfarm @pride_portland #equality #loveyou #gaylyownedfarmstore Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-53444664586966681802017-06-11T14:52:00.001-07:002017-06-25T12:04:34.541-07:00Great Selection of Summer Vegetable Starts!! (plant list updated 6/25/17) We still have a great selection of summer vegetable and herb starts. To check out our current availability, click HERE!! Whether you're just planting or found room for juuussssstttt one more tomato plant, we're here to help! If you're new to the area and think it's too late to plant, don't be shy! Come on in and let us help you get your garden growing for the season. From soil and fertilizers Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-89253664505505182342017-05-27T07:30:00.003-07:002017-05-27T09:37:47.085-07:00Open on Memorial DayIf your three day holiday weekend is full of garden & farm time, we're here for any plants and supplies you need. Open normal 10-6, including Monday. 💕 Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-53355211809627050882017-05-18T13:38:00.002-07:002017-05-18T13:45:38.313-07:00Heat and Sunshine! (aka winter to summer...) Goatlers are standing by to "help" you with your plant boxes + pics of scrumptious nursery land!! We're ready for you, the heat and sunshine. More landing tomorrow + meet one of our new team elves, Darin! He farmed with 47th Ave Farm and now farms with The Side Yard Farm & Sweet Delilah Farm and at our shop. Oodles of love and warm weather wishes! Naomi ❤❤❤ #wintertosummer #seportland #Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-38645728078426736492017-05-04T08:33:00.000-07:002017-05-06T16:15:13.053-07:00Bunny Spa Day at Naomi's on May 7th + PLANTS Our next Bunny Spa Day is coming up this Sunday from 12 - 3. It tends to be busier early, so coming a bit on the later side may be good. We will have a market tent set up at the back of our building. We have very ample parking in the back / south side of our shop on weekends and highly encourage you to park there if you are bringing your bunny in. Volunteers from Rabbit Advocates will be Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-38572619263251450272017-04-16T10:10:00.001-07:002017-04-16T10:10:56.469-07:00Feed your bunnies so many fresh greens every day and this happens. 🐰🌈🐰 We're open 10-6 for a really dreamy gardening day if you need supplies. #rabbitadvocates #bunnyloveNaomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-78948552936891871932017-04-05T14:20:00.001-07:002017-04-05T14:42:45.076-07:00Flower Bouquet of Eggs & Chicks If eggs were a flower bouquet... We have another 30 dozen of these landing this afternoon & baby chicks came in this morning if you want to tend your own rainbow of egg layers. All fed organic @scratchandpeck feed! We have loads of veggies, gorgeous flowers, natives and sooo much fruit in stock. #hendiversity #8yearsofchicks #organicfromday1 #muddogfarm Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-52934827317284606522017-03-23T02:53:00.001-07:002017-04-05T13:57:13.464-07:00Baby Chicks! our third batch of fluffy peepers landed Wednesday morning!! 🌈🐥🌈 #babychicks #organicfromday1 #scratchandpeck (Leslie helping a new chickie family!) Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-28794904688886842272017-03-04T12:22:00.001-08:002017-03-04T12:22:08.922-08:00Great signs & Bunny PawdicuresBunny Spa Day this Sunday 12-3!We've got both of these available for you in the shop at cost💛💛💛 Black Lives Matter yard signs are $6.50 and stickers .90 cents. Designed by Mattice Moore, you can also buy directly through www.peacesupplies.org. 🌈We Welcome All signs are $7, we donated $5 each to @iprc_pdx and got them laminated for $2 at Minutemen Press (who used to be in our bldg). You can Naomi Montacrehttps://firstname.lastname@example.com:blogger.com,1999:blog-8254259984755193981.post-26841116008807900992017-02-23T13:07:00.004-08:002017-02-25T11:02:34.824-08:00First Baby Chicks are here! a Our first batch of spunky baby chicks are in!! This is our 8th year using organic feed and lots of love. Find more about our practices, pre-ordering and available beeds for walk-ins on the 'Baby Chicks!' section website: 🐣🌱🌷 (📷 of Becca by Naomi) I was invited to the @aclu_nationwide of Oregon dinner last night by one of our customers. So inspiring!! #dahlialithwick gave me chills. Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-78857016683027571622017-02-18T10:04:00.001-08:002017-02-19T10:21:38.414-08:00First of 2017 veggies, plus crowns and flowers Our first round of early veggies and herbs are here and will be expanding each week til summer is in full swing! More annual flowers in, too, since we're all craving colorful blooms a little more than usual this year...!! Check out our varieties of flowers, herbs and vegetable starts here. Mason Bee cocoons are in, along with different tubes and neat houses. First chicks arrive FridayNaomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-61658787872555787712017-02-05T18:34:00.002-08:002017-02-05T18:34:57.806-08:00Super Bunny, Super Bowl Move over boys, Cinnabun's gonna win this. And perform with Lady Gaga. nbd. 🐰🏈💘 #mvp #superbunny #superbowl 📷 Susan C.& Gwen B.Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-39004788565598650982017-01-22T09:44:00.003-08:002017-01-22T15:51:23.466-08:00Bunny Spa Day! Today from 12 to 3pm at our shop, it's Bunny Spa Day! Our next one is Sunday, March 5th, same time and bi-monthly all year. Volunteers from Rabbit Advocates will be here for Nail Trims & Grooming, including: quick look, light mat removal, scent gland cleaning and gender ID for adult rabbits - $5 donation each. Add-ons: long haired rabbit detangling, fur trimming and de-matting & poopy Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-79104422798000947072017-01-21T10:38:00.001-08:002017-01-23T13:23:58.145-08:00Women's March! Saturday 1/21 - Opening later Portland, Oregon, over 100k! Adaptive Seeds! Due to the Portland Women's March, we are opening later today, at 1pm, we'll update here and our voicemail. ❤❤❤ Check out theses incredible, inspiring photos: Pictures from Marches on Every Continent PDX March in the NY Times! Moon River Farm! Our Table Cooperative Farm! Uprising Seeds at the Bellingham March! We are just getting Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-8506893516917603482017-01-18T15:31:00.001-08:002017-01-18T15:31:50.988-08:00Thaw! and 8,180 Seed Packetschecking in 8,180 seed packets from @uprising.seeds with two of my faves @foxandbearurbanfarm and @herbnvine, drinking hot bevs, being goofballs and loving every moment of rainy 40's weather.☕🌧😻💪🌱💕 also unloading lots of 50# bags as trucks are delivering again after a week and a half of ice 'n snow.🚚🚜 #thebigthaw #ladyguys #womenownedbusiness #womenrunbusiness thanks for the special Naomi Montacrehttps://email@example.com:blogger.com,1999:blog-8254259984755193981.post-16707696016680626492017-01-17T10:04:00.001-08:002017-01-17T12:41:54.777-08:00Ice Storm - Opening at 10am, short day *Closing at 1pmHi there! Due to extra icy potential today, we'll be open at 10am and closing if/when the weather changes = 1pm, as the other shops around us have all started closing early and public transit is slow, using chains and stopping routes. Can't wait for the 'normalcy' of good ol' 40's and rain to return. Stay safe!!Naomi Montacrehttps://firstname.lastname@example.com


text:
Score from 57 reviews
Guest accommodation
Guest accommodation
from 100,8 USD – Apartment
0.07 km
Rtić 3, 4 22202, Primošten
0.09 km
Rtić III 28 22202, Primošten
0.09 km
22202, Primošten
0.09 km
Rtić III 28 22202, Primošten
* Distances are measured as a straight line, calculated from a starting point to a destination point
All bookings are confirmed instantly. No waiting, no email exchanges, no confusion.
Offering an outdoor heated pool, this hillside property is 3 km from the town of Primošten and 150 metres from a rocky beach of the Adriatic Sea. It offers air-conditioned apartments with free WiFi and a balcony with sea views.
A well-equipped kitchenette with a fridge, cooking hobs, a dishwasher and an electric kettle are featured at Apartments Martina. Each has a dining area, as well as a sofa bed and satellite TV.
Guests can enjoy access to BBQ facilities, along with free covered parking at Martina Apartments. Laundry service is available upon request and at a surcharge.
A variety of restaurants, bars and shops can all be found within 800 metres. Trogir is 30 km away, while Split is 60 km from the apartments. Krka National Park is about 40 minutes' drive away.
Rooms: 5
Please inform Apartments Martina in advance of your expected arrival time. You can use the Special Requests box when booking, or contact the property directly with the contact details provided in your confirmation.
Refrigerator, Electric kettle, Kitchenware, Dishwasher, Kitchen, Dining area, Stovetop, Dining table
Toilet, Bathroom, Shower, Toilet paper, Bath or Shower
Heating, Air conditioning, Hardwood/Parquet floors, Sofa bed, Private pool, Private entrance, Sofa, Ironing Facilities, Wardrobe/Closet
Flat-screen TV, TV, Satellite Channels
Linen, Towels
Pool view, Sea view, Balcony, Patio, View, Garden view
Spacious apartment with 2 bedrooms, a kitchenette, dining area and sofa bed. Features a covered balcony with sea views.
Dining table, Stovetop, Kitchenware, Dishwasher, Dining area, Electric kettle, Kitchen, Refrigerator
Shower, Bathroom, Bath or Shower, Toilet paper, Toilet
Private entrance, Air conditioning, Sofa bed, Ironing Facilities, Wardrobe/Closet, Hardwood/Parquet floors, Heating, Private pool, Sofa
Flat-screen TV, Satellite Channels, TV
Towels, Linen
Garden view, Balcony, View, Patio, Pool view
Entire unit located on ground floor
Spacious apartment with 2 bedrooms, a kitchenette, dining area and sofa bed. Features a covered balcony.
After booking, all of the hotel's details, including telephone and email address, are provided in the online booking confirmation, confirmation email.
WiFi available in all areas
Cycling, Fishing, Beach
BBQ facilities
Beachfront, Outdoor pool, Heated pool, Swimming pool, Fence around pool
Garden, Sun terrace, Outdoor furniture, Terrace
Ironing service, Laundry
Family rooms, Heating, Air conditioning
Free WiFi! WiFi is available in all areas and is free of charge.
Free parking! Free private parking is possible on site (reservation is not needed).
From 14:00 Until 20:00 hours
From 07:00 Until 10:00 hours
All children are welcome.
All children under 19 years are charged EUR 15 per person per night for extra beds.
All adults are charged EUR 15 per person per night for extra beds.
All children under 1 year stay free of charge when using existing beds.
There is no capacity for cots in the room.
The maximum number of extra beds in a room is 2.
Pets are not allowed.
This property only accepts cash payments.
Our hotel reviews will help you find the best deal in the right location.
ihr24.com offers the choice of over 600,000 hotels in more than 200 countries.
Sign up for your free My ihr24.com account
Website in English and 17 other languages


text:
Los Alcazares Los Alcazares
Open from 12.00 to 24.00, Monday to Sunday, 365 days of the year
We have a wide variety of pastas, pizza, meat, fish, salads ... . Moreover, Spanish cooking, grill and paellas meat and fish.
And also take away food
Enjoy our desserts: pudding, tiramisu, Macedonian and cakes
Welcome birthdays, communions, baptisms and business dinners in Los Lacazares
I will gladly answer them, don´t hesitate and call us . Los Narejos
|We Love Los Alcazares
|Av. Rio Piguera nº 27 Los Narejos - Los Alcazares
|Reservation Telephone: 968 574 288
recomended by areadenoche.es - areaderestaurantes.es - areacabopalos.com - netareas.com - cabodepalos.com - elmarmenor.com - areamarmenor.es - areabares.Es - Areadecartagena.com


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Hi! I am pregnant and freaking out that something is wrong. Been a bit dizzy a few times but that is it...I am so scared that I am losing the baby again.
Last time I lost baby after a surprise scan at my doctors and there was no heartbeat at 10 weeks. The baby had died at 9 weeks.
What was your experience this early? Any symptoms at all? I did a blood test today but wont find out until tomorrow if it the levels are good.
Anyone want to share their experience or if you felt the same way????
This wait is killing me
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15-11-2012 13:54 #1Junior Member
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5 weeks 3 days Pregnant
15-11-2012 14:12 #2Senior Member
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I had two miscarriages before my son that was about 6 years before when i was quite young unplanned and different partner one was a missed miscarriage and found at the 12wk scan my baby had died at 6wks and when i got pregnant with my son i was so worried it would happen again as i didnt have that many symptoms but i went for regular blood test for first few weeks to put my mind at ease then a scan at 6wks and then 12 wks then once i reached that point i relaxed more and had a healthy pregnancy that was 2 years ago then i just got pregnant a few months ago but sadly miscarried a few weeks ago at 7wks i guess there is no point stresssing because we really have no control over it i know that the babies i lost must not have been healthy to begin with and im happy it happened sooner in the pregnancy rather then later. Im sure your pregnancy will be just fine good luck and try to relax and think positive.
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text: The list of Dev Airlines destinations Contents[show] Current Destinations Edit Hubs Edit Detroit Metro (DTW) Focus Cities Edit Flint-Bishop (FNT) Future Destinations Edit London-Gatwick (LGW) Grand Rapids (GRR) Michigan Tri-Cities (MBS) Toledo (TOL) Retrieved from "http://dev-airlines.wikia.com/wiki/Dev_Airlines_Destinations?oldid=140" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Add category Cancel Save


text:
de femmes,
Femmes de lettres"
Directed by Henrik Borgstrom
Performed by Francine Conley
Students: $5.00
"Lettres de femmes, Femmes de lettres" ("Letters by
Women, Women of Letters") is a visually engaging
production adapting writings by French and Francophone
women authors from across six centuries, from Marie de
France to Fatou Diome, including Madame de la Lafayette,
Colette, and Maguerite Duras. All of these women and
their selected texts share an "imagery pertinent to the
natural elements of water, wind and fire as well as
themes of letters, paper, voyage, exile, and [...] the
need to write oneself out of exile and silence."
Over a background of images and quotes, "Lettres de
femmes, Femmes de lettres" reveals the humanity of
women's voices over the centuries. Through a visual trip
through space and time, visiting the far away reaches of
the French-speaking world, as well as its distant
centuries, leading up to today's complex reality, the
audience will relate to the emotions and dilemmas of
famous female writers expressing their views as mothers,
lovers, sisters, and daughters. Such authors include
Marie de France, a poet who settled in England in the
shadow of William the Conqueror; Madame de Lafayette,
who wrote the very first modern novel; Colette, a
provocative writer who broke taboos in early 20 century
France; and Marguerite Duras, whose sensual experience
of life in Vietnam marked generations of French teen
Directions, Map & More Info.
13406 E. Philadelphia St.
Whittier, CA 90608 562.907.4200


text:
Grand Bras, St. Andrew,
Cozy one bedroom, one bathroom fully furnished downstairs apartment.
Open concept living, dining and kitchen area secured with bars on windows.
A private verandah with built-in seating is located at the back.
The town of Grenville is only 5 minutes drive and there are schools, churches, gas stations and shops around the vicinity.
Address: Grand Bras
City: St. Andrew
Country: Grenada
258 USDper month
Property ID: R536
Bedrooms: 1
Bathrooms: 1
Air Condition
Back yard
Bars on windows
Electric Water Heater
Fenced yard
Front yard
Fully Furnished
Scenic Views
Screened Windows
Water Tank
My details
Real Estate Broker
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text:
The cat eye stylized Get the sexy kitty cat look, here is how to do it !
Behind Blue Eyes - Just a point of fact - most of these cat eyes are Photoshopped by morons who don't know what color eye goes with what breed etc. Some are very pretty, just not natural.
Cat Eye liner for hooded eyes. How to apply a cat eye for your eye shape - hooded eyes.
They have the most amazing eyes, don't they? What color are your cats' eyes?


text:
The Coast / St. Marys
Bird Brains Nature Camp
Dates : 1/15/18 - 1/15/18Crooked River State Park
6222 Charlie Smith Senior Highway
St. Marys, GA 31558
School's out and parents are invited to send their K - 8th graders to nature camp. This camp is for all the bird brains out there that are interested in learning more about our feathered friends. We will explore the birds that call Crooked River their home and discover their habitats. Limited space, pre-registration required.
Info & Amenities
Hours of Operation
Monday: 8:00AM - 4:00PM
Admission & Fees
Admission Fee $10 half day or $25 full day.
Parking Fee $5
Outdoors & Nature
State Park Events


text:
- Which Commodities Depend the Most on Exports?
- Statement Of Ag Sec'y Perdue Regarding Trade Breakthrough With China
- U.S. Beef Headed to China
- U.S., China Agree to First Trade Steps Under 100-Day Plan
- 70 Percent Chance Dec. Corn Futures Hit $4.40-$4.50
- DC Dialogue: Trade for Rural Development?
- Fed Budget Cuts: Ag-Related Agencies Look for Alternative Funds – DTN
- Perdue Heads to Hill, USDA Nominees Near
- The Next Month Will Be Critical for Disease Development
- EPA Reopens Comment Period for Pyrethroids
- Investment in AgTech Have Tripled Over 2016
- Cargill's CEO Expects World's Grain Supply To Remain Oversupplied
- USDA: Exports Account For More Than 50 Percent Of U.S. Cotton And Almond Production
- 2017-18 U.S. Cotton Crop Could be Biggest in 11 years
Subscribe to T&C Trending Now Email Alerts
Federal Government Links
Farm & Other Media Links
Southwest Farm Press
- Maintenance in the shop and field could go easier with these products November 23, 2017
- Acknowledge contributions of farmers this Thanksgiving November 23, 2017
- National Cotton Council introduces Northern, Midwest growers to High Plains agriculture November 23, 2017
- 19 appointed to United Soybean Board November 22, 2017
- National Cotton Council tour gives perspective November 22, 2017
Search Our Site


text:
OBSERVATIONS: Agency Theory as a Framework for Advertising Agency Compensation Decisions
R Susan Ellis, and Lester W Johnson,
University of Sydney, Australia
Advertising agencies have historically received the majority of their compensation either through commissions or through some sort of fee structure (Shimp, 1990). Under the commission system, a flat percentage (traditionally 15 to 16.67% but now sometimes lower) is paid to the agency on the value of all advertising placed in the major media (broadcast, print, and outdoor), and a similar commission is added onto the cost of producing the advertising and to out-of-pocket expenses. In contrast, the fee system results in the agency being paid either a fixed fee or an hourly rate for services performed and in some cases a mark-up or commission on outside services. Each of these types of compensation plan can, under certain circumstances, lead to friction between the advertising agency and its client (Zeltner, 1981; Shimp, 1990).


text:
Books by this Author
My Life in 8 Words
Author Revealed
Q. What is your motto or maxim?
A. breathe, breathe again
Q. What trait is most noticeable about you?
A. in whose eyes? children, friends, collegues?
Q. If you could meet any historical character, who would it be and what would you say to him or her?
A. Virginia Woolf
Mrs Woolf, having met you at an impressionable age, you changed my view for a long long time.
Q. Who is your favorite fictional hero?
A. Madame Bovary
Q. What is your favorite occupation, when you're not writing?
A. walking, talking with dogs, dinner with friends


text:
Miss 3.5 is allergic to soy, almonds and egg
Miss 7 months is allergic to dairy, sesame and wheat.
We are now totally on a fruit, veggies and somewhat meat diet. I'm struggling and i mean the water is above my head and i can't get to the top.
Both kids are undergoing a skin prick test on the 10th oct. I don't think i have ever wished my life away but hurry up.
So far i have handled the kids allergies with ease (even daycare with miss 3) now meet soy. I swear that stuff is in everything. We all are starving. Miss 3 is basically what this post is about. Soy absolutely sends her crazy and i don't mean tantrums i mean full on psychotic. She does this for about 4 hours after eating her eyes swell she vomits like the exorcist swears, bites, punches and tries to get knives (which i have put up) because she says she wants to kill us. She gets a rash and hives all over.
I am at the end of my rope. I have cut soy out of our diets which is in everything. Even her multivitamin has soy ( tocopheryl) our baby wipes have soy and our shampoo.
I guess i just need to get it off my chest I'm struggling so bad. Husband is fifo and he sees the difference. Last night he even said i want my little girl back
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28-09-2016 12:26 #1
soy allergy
28-09-2016 12:43 #2Member
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Oh man I'm feeling you.. I'm bfing my little one who seems to have issues with Dairy, egg and soy so I'm in the hungry boat too! I just can't believe how much food etc has soy in it. I have a sneaking suspicion that gluten may be causing her drama too. I have been eating fruit, vegetables, Turkish bread and dark chocolate. I googled vegan baking and it looks like there are some yummy recipes. Not much help but you're not alone!
Hahaha the most delicious thing I ate last week was potatoes cut into chunks roasted in the oven with a little olive oil and salt
28-09-2016 16:19 #3
Thanks for your reply. I can't eat wheat or dairy or sesame either because I'm breastfeeding.(nooo chocolate) I thought surely it can't be true i snuck a bite of cheese. Bub covered from head to toe in rash with explosive poops yep won't try that again.
My main diet is breakfast corn thins with jam. Lunch salad. Dinner rice and veg. Although i have just made awesome bliss balls dates coconut cocoa dried apricots and coconut oil. Yep they are pretty good.
We can never eat out again (sad face) even just walking into a bakery little bub breaks out. Miss 3 gets contact rashes and hives just from the tables.
I feel like i have done something wrong. I feel like a bad mum. We tried for 6 years before we were blessed. And now this. ..
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text:
Wyandotte County, KS
If you are seeking fun and frolic, then you'll surely enjoy the 30 annual festivals in this City of Festivals. The grandest of the festive events is the Renaissance Festival. The Renaissance Festival lasts seven weekends in the fall, returning visitors to a time of knights and fair maidens, a time long, long ago. The festival is rated as one of the "Top 100 Events in North America." The Woodlands offers dog and horse racing, and experience Kansas Speedway "The track that will blow you away." Bringing the hottest entertainment to town is the cool, outdoor Verizon Wireless Amphitheater. If you have a favorite group, they're sure to hit Verizon some time in the summer. The Agricultural Hall of Fame, Grinter House, Huron Indian Cemetery, Wyandotte County Museum and the Rosedale Arch represent the historic sites within Kansas City, Kansas and Wyandotte County.
Wyandotte County, the smallest county in Kansas with 155.7 square miles of land, was incorporated in 1859, and was made up from portions of Leavenworth and Johnson counties.
Anthropologists believe that the confluence of two rivers— the Kansas and the Missouri— was a significant factor in attracting the first primitive Indians some 2,000 years ago. In 1825, the federal government moved the Shawnee Indian tribe to Wyandotte County. The area had been under control of Spain and later France. However, the United States acquired the area as part of the Louisiana Purchase in 1803. In 1830, the federal government moved the Delaware Indian Tribe to Wyandotte County. Then in 1843, the Wyandotte Indians came here from Upper Sandusky, Ohio. The Wyandotte Tribe took an active part in community affairs. The chief, William Walker, became the first provisional governor of the Kansas-Nebraska Territory in 1854. Most of the Indians accepted U.S. citizenship. A few moved to Indian territory in Oklahoma. Today the official home of the Wyandotte Tribe of Oklahoma is in Wyandotte, Oklahoma.
The first permanent white settler in Wyandotte County was Moses Grinter. He built Grinter House in 1857 which is now a state historical site which overlooks the Kansas River. Grinter was chosen by Col. Henry Leavenworth to operate the ferry across the Kansas River. Col. Leavenworth, who had established a military post, needed a ferry to link various military outposts that stretched from Ft. Snelling, Minnesota, to Ft. Gibson, Oklahoma. These military posts were necessary to protect the stream of traders and travelers heading down the Santa Fe Trail and to preserve peace with the Indians. Grinter started operating the ferry in 1831. Travelers, both civilian and military, paid 50 cents a person or $2 a wagon to cross the river.
Explore Wyandotte County
Bonner Springs High School
The Bonner Springs High School, listed on the National Register of Historic Places, was built in 1918 and was in continuous use until 1982Bonner Springs, KS Historic Schoolhouses
National Agricultural Center & Hall of Fame
Federally chartered, privately funded national museum of agriculture. This unique 172 acre complex features National Farmer's Memorial, National Agriculture Hall of Fame, Museum of Farming, Gallery of Rural Art, and a turn-of-the-century rural village, Farm Town USA, complete with 1Bonner Springs, KS Museums
VerrĂĽckt -- World's Tallest Waterslide!
VerrĂĽckt, the World's Tallest Waterslide opening in 2014 to guests of Schlitterbahn Waterpark Kansas City, was officially verified by Guinness World Records prior to its opening. The VerrĂĽckt stands at a record-breaking height of 168 ft 7 in. After the initial, nearly vertical drop of 168Kansas City, KS Water Parks
Anthology of Argentine Wall
The prevailing spirit of Argentine and its people inspired local artists to document the life and times of residents of the Argentine area with a mural that is 220 yards in length and its highest point is 30Kansas City, KS Arts
Wagner's Classic Cars
For the car enthusiast, this dealership has a showroom dedicated to restored cars from the 1920s up to the 1970s. The showroom is filled with collector cars, muscle cars, street rods & trucks of all kinds. Wagner'Bonner Springs, KS Museums
White Church Christian Church
White Church Christian Church was established in 1832 and is known as the oldest church in the state of Kansas that is still in use. The native stone church includes 21Kansas City, KS Historic Churches
Korean/Vietnam Veterans Memorial of Wyandotte County
The nation's first dual war memorial is dedicated to veterans of both the Korean and Vietnam conflicts. The marble memorial with two life-size bronze statues was formally dedicated on Veterans Day 1988Kansas City, KS Memorials
Dub's Dread Golf Course
We're going back....back in time when Jug McSpaden, creator of Dub's Dread, had a vision. That vision was to create a golf club that offered a superb design, premier playing conditions and outstanding service. Dub'Kansas City, KS Golf Courses
Moon Marble Company
A delightful store full of marbles and other games. Antique marble toy display. Gift shop sells marbles, nostalgic, and unique toys. Call ahead for times on when to watch marbles being made.Bonner Springs, KS Venue Attractions
Children's Museum of Kansas City
The Children's Museum is a hands-on learning center designed for families and children ages 2-8. Interactive discovery-based exhibits channel curiosity, promote problem-solving skills, and teach various aspects of art, science, history and daily life.Kansas City, KS Learning Centers
Wyandotte County Lake and Park
WyCo Lake and Park provides 1500 acres of wooded area, a 400Kansas City, KS Recreation


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Bạn cần tìm Freelancer hoặc công việc Inno setup run vista?
Cần giúp đỡ với Inno setup run vista? Thuê một freelancer ngay hôm nay! Bạn có chuyên về Inno setup run vista? không? Hãy sử dụng kĩ năng Inno setup run vista? của bạn và bắt đầu kiếm tiền trực tuyến ngay hôm nay! Freelancer là thị trường công việc lớn nhất trên thế giới. Hiện tại có 17.764 công việc đang đợi bạn đặt giá!Tôi Muốn Thuê Người Tôi Muốn Làm Việc
Công việc Inno setup run vista:
|Dự án/Cuộc thi
|Mô tả
|Lượt đặt giá/Bài dự thi
|Kĩ năng
|Bắt đầu
|Kết thúc
|Giá (VND)
|loại hình
|Dự án/Cuộc thi
|Mô tả
|Lượt đặt giá/Bài dự thi
|Kĩ năng
|Bắt đầu
|Kết thúc
|Giá (VND)
|Auto press hotkey shortcuts when a file location is played. I have to run into Windown.
|I want to build a script in AHK. It could be auto press hotkey shortcuts default when a file location is played. This file location could be file .wav or something like that. Actually, this file location might could control to a software runing in computer. Finally, feature of this scripts is press hotkey shortcuts default when a file location opened to software in computer. [Removed by admin] We ...
|Java, Javascript, Visual Basic, Lập trình C#, Lập trình C++
|Nov 18, 2017
|Hôm nay6n 7g
|Make my Facebook, Google and Twitter Ads Run
|I have set up Ads in these channels and all have been approved, but none is running. I need them optimized and start running.
|Tiếp thị qua Internet, Google Adwords, Quảng cáo trên Facebook, Quảng bá truyền thông, Twitter Marketing
|Nov 18, 2017
|Nov 18, 20175n 23g
|Make my Google Facebook and Twitter Ads Run
|I have set up my Google, Facebook and Twitter Ads and all have been approved but none is running. I need to optimize them so they can start running.
|Tiếp thị qua Internet, Google Adwords, Quảng cáo trên Facebook, Quảng bá truyền thông, Twitter Marketing
|Nov 18, 2017
|Nov 18, 20175n 22g
|Need an android app which always run in background.
|Make a activity run every 15 minutes, even if app is closed or killed it should open on its own and it should always run in background.
|PHP, Java, Mobile App Development, Android, Kiến trúc phần mềm
|Nov 18, 2017
|Nov 18, 20175n 21g
|make program that run in parts in several machines running linux (server part) and windows (client part, GUI)
|make program that run in parts in several machines running linux (server part) and windows (client part, GUI)
|Python, Linux, Thuật toán, Ubuntu, Lập trình cơ sở dữ liệu
|Nov 16, 2017
|Nov 16, 20174n 10g
|Love On The Run
|I am looking for a good person to outline (in detail) a lgbt romance novel.
|Viết quảng cáo, Ghostwriting, eBooks, Viết sách, Viết sáng tạo
|Nov 16, 2017
|Nov 16, 20174n 3g
|Setup a PHP script to run on windows
|We are looking for someone to setup a PHP script to run on windows. Experience scraping websites is a bonus.
|PHP, Python, Web Scraping, Kiến trúc phần mềm, MySQL
|Nov 15, 2017
|Nov 15, 20173n 9g
|Run a program on a timer in windows and remote management setup
|Very Simple task. I need someone that can do the following: -set my computer up in order to run "NiceHash" program on a timer. It will be running 24/7 however there are certain hours I do no want it running, so I would like you to set that up. -set up a way I can remotley manage my computer, with someway that a program can "ping" or check with the computer that it is mini...
|Lập trình C, Màn hình Windows, Lập trình cơ sở dữ liệu, Tự động trong nhà, Programming
|Nov 15, 2017
|Nov 15, 20173n 7g
|Make a C++ program that must run on visual studio
|I have uploaded a file below that will tell you what exactly you would need to do ! It's a simple project that shouldn't take you more than an hour if you are good in programming but I will give you 2 days to complete it if you guarantee to deliver me !
|Lập trình C, .NET, Lập trình C#, Lập trình C++
|Nov 15, 2017
|Nov 15, 20172n 23g
|run simulation on simulink
|I need someone to run a simple simulation on matlabs Simulink for me with various inputs.
|Kĩ thuật, Điện tử, Matlab and Mathematica, Verilog / VHDL, Kĩ thuật điện
|Nov 14, 2017
|Nov 14, 20172n 13g
|CRM AND DATABASE GURU - BUILD AND RUN DBs ON GOOGLE CLOUD OR AWS
|CRM DATABASE GURU - BUILD AND RUN A CRM DATABASE ON GOOGLE CLOUD OR AWS Initial input: LinkedIn first connections, Facebook friends, Gmail address book Additional input from our Research team (lists, graphs, descriptions) YOUR GURU-LEVEL EXPERTISE: CRM database setup and management (OpenSource, Salesforce, Insightly, Others) Data Extraction, Data Mining, Screen Scraping, Web Crawling ...
|CRM, Dịch vụ trang web Amazon, Quản trị cơ sở dữ liệu, Lập trình cơ sở dữ liệu, Phát triển cơ sở dữ liệu
|Nov 13, 2017
|Nov 13, 20171n 4g
|Need to setup AWS server in Amazon, to run cakephp application
|The person should have complete knowledge of linux server of AWS AMAZON how to handle it and need to point the IP to a domain with SSL certificate, so you should also know how to activate SSL in the server
|PHP, Quản trị hệ thống, Linux, MySQL, Dịch vụ trang web Amazon
|Nov 13, 2017
|Nov 13, 20171n 1g
|i need to make a program/robot that can run while my computer is turned off like on amazon services
|i need a programmer who is able to make a robot which i can run on amazon services.(still be running while my computer is off) . the program can exchange my money to the cryptocurrency which value is increasing by micro_units on [url removed, login to view]
|Nov 12, 2017
|Nov 12, 201720g 2t
|Build Website and run SEO
|Build Website and run SEO first page google
|PHP, Thiết kế trang web, Tiếp thị qua Internet, SEO, Xây dựng liên kết
|Nov 12, 2017
|Nov 12, 201712g 45t
|Run my instagram
|Run my instagram Message for more details
|Mobile App Development, iPhone, Android
|Nov 12, 2017
|Nov 12, 201711g 59t
|Need someone to run already finished script
|The project is really simple. I have a script that needs to be runned. The only thing that you need to do is to enter some URL-s that needs to be scraped. Document with explanations how it works will be delivered.
|Python, Web Scraping, Programming, Scrapy, Data Scraping
|Nov 12, 2017
|Nov 12, 20176g 10t
|Run JS Script when clicking on a button that redirects to a url
|I need somebody to help me to create a button that redirects to a URL (my campaign url) and that also calls a JS script when somebody clicks there.
|PHP, Javascript, HTML, jQuery / Prototype, Google Chrome
|Nov 11, 2017
|Nov 11, 2017Đã kết thúc
|Forex automated trading algorithm based python to run with Oanda
|I would need somebody to make the python for Oanda that should open and close transactions in Oanda automatically based on a set of rules (indicators) I would consider the project successful when the algorithm runs and is backtested without errors. I appreciate honest bids from people that have had experience with this before. Have an awesome day! :)
|Python, Metatrader
|Nov 11, 2017
|Nov 11, 2017Đã kết thúc
|Design a Logo For Vista Digital Google Trusted Agency
|Hello everyone ! I need to have logo designed for Vista Digital Second Line Should say Google Trusted Agency Company Making 360 virtual tour for google I belive in you ! Thank you
|Thiết kế đồ họa, Thiết kế logo
|Nov 10, 2017
|Nov 10, 2017Đã kết thúc
|Setup and run iOS source code based on C2Call SDK
|I need a good skilled iOS programmer to make possible run and archive as IPA file a free source from C2Call. The C2call SDK have a free project for Chat named Wazzupp and I can not make it works. See [url removed, login to view] for more details. Their SDK is free and you can create a free account, open developer area, and download their SDK for iOS. * IMPORTANT - Do not bid on my projec...
|Mobile App Development, iPhone, Objective C, iPad, Swift
|Nov 8, 2017
|Nov 8, 2017Đã kết thúc
Showing 1 to 20 of 6848 entries
Thuê những Freelancer Inno setup run vista hàng đầu: