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S&P 500 (NYSE:SPY) component Peabody Energy (NYSE:BTU) will unveil its latest earnings tomorrow, Tuesday, January 29, 2013. Peabody Energy mines steam coal for sale mainly to electric utilities and metallurgical coal for sale to industrial customers.
Peabody Energy Earnings Preview Cheat Sheet
Wall St. Earnings Expectations: The average analyst estimate is for net income of 26 cents per share, a decline of 80.6% from the company's actual earnings for the year-ago quarter. During the past three months, the average estimate has moved down from 28 cents. Between one and three months ago, the average estimate moved down. It has been unchanged at 26 cents during the last month. For the year, analysts are projecting profit of $1.97 per share, a decline of 52.8% from last year.
Past Earnings Performance: The company has beaten estimates the last four quarters and is coming off a quarter where it topped forecasts by 17 cents, reporting net income of 51 cents per share against a mean estimate of profit of 34 cents per share.
Start 2013 better than ever by saving time and making money with your Limited Time Offer for our highly-acclaimed Stock Picker Newsletter. Click here for our fresh Feature Stock Pick now!
A Look Back: In the third quarter, profit fell 84.3% to $42.9 million (16 cents a share) from $274.1 million ($1 a share) the year earlier, but exceeded analyst expectations. Revenue rose 1.1% to $2.06 billion from $2.04 billion.
Here's how Peabody Energy traded following its last earnings report 3 months ago and leading up to its upcoming earnings report this week:


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Air Analyst is very similar to another occupation for which we have
a full profile.
Check out our full profile for:
Environmental Science and Protection Technicians
Performs laboratory and field tests to monitor the environment and investigate sources of pollution, including those that affect health. Under direction of an environmental scientist or specialist, may collect samples of gases, soil, water, and other materials for testing and take corrective actions as assigned.

subset: commoncrawl


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Document Actions
Oil and Gas
Ceres oil and gas related reports
A Framework for 2 Degrees Scenario Analysis: A Guide for Oil and Gas Companies and Investors for Navigating the Energy Transition
Dec 06, 2016
- This report provides oil and gas companies and their investors with a framework to assess the resilience of company portfolios to climate- and technology driven shifts in demand, and to provide decision-useful insights that will help companies mitigate the vulnerabilities they face as energy markets transition to a low carbon future.
Accelerating U.S. Clean Energy Deployment: Investor Policy Priorities
Sep 08, 2015
- International investment to mitigate climate change is far below levels needed to reach the two-degree target. The International Energy Agency estimates that an average of an additional $1 trillion in incremental financing for clean energy is needed to meet the temperature target.
Carbon Asset Risk: A Review of Progress and Opportunities
Jun 30, 2015
- n September 2013, Ceres and the Carbon Tracker Initiative launched the Carbon Asset Risk ("CAR") Initiative with support from the Global Investor Coalition. This report chronicles major shifts in the financial landscape since the CAR effort began.
Hydraulic Fracturing & Water Stress: Water Demand by the Numbers
Feb 05, 2014
- This Ceres research paper analyzes escalating water demand in hydraulic fracturing operations across the United States and western Canada. It evaluates oil and gas company water use in eight regions with intense shale energy development and the most pronounced water stress challenges.
Investing in the Clean Trillion: Closing The Clean Energy Investment Gap
Jan 15, 2014
- In 2010 world governments agreed to limit the increase in global temperature to two degrees Celsius (2 °C) above pre-industrial levels to avoid the worst impacts of climate change. To have an 80 percent chance of maintaining this 2 °C limit, the IEA estimates an additional $36 trillion in clean energy investment is needed through 2050—or an average of $1 trillion more per year compared to a "business as usual" scenario over the next 36 years. This Ceres report provides 10 recommendations for investors, companies and policymakers to increase annual global investment in clean energy to at least $1 trillion by 2030—roughly a four-fold jump from current investment levels.
Flaring Up: North Dakota Natural Gas Flaring More Than Doubles in Two Years
Jul 29, 2013
- The tremendous growth of unconventional oil production in North Dakota has also led to a rapid rise in the production of associated natural gas. However, state authorities report that a large percentage of this gas does not ultimately go to market. Nearly 30 percent of North Dakota gas is currently being burned off, or flared, each month as a byproduct of oil production.
Hydraulic Fracturing & Water Stress: Growing Competitive Pressures for Water
May 01, 2013
- This Ceres research paper analyzes water use in hydraulic fracturing operations across the United States and the extent to which this activity is taking place in water stressed regions. It provides an overview of efforts underway, such as the use of recycled water and nonfreshwater resources, to mitigate these impacts and suggests key questions that industry, water managers and investors should be asking.
Investor Expectations for Improving Environmental & Social Performance in Canadian Oil Sands Development
Oct 22, 2012
- A group of 49 investors with $2 trillion in assets under management are calling on Canadian oil sands developers to dramatically reduce the environmental and social impact of their operations.
Sustainable Extraction? An Analysis of SEC Disclosure by Major Oil & Gas Companies on Climate Risk and Deepwater Drilling Risk
Aug 02, 2012
- Disclosure of material business risk is a core underpinning of the modern global economy's health. A new report says that investors aren't getting a clear picture from companies of just how deep the material risks are.
Investor Risks from Oil Shale Development
May 30, 2012
- May 2012 - The Department of the Interior's Bureau of Land Management (BLM) recently proposed limiting federal leases for development of oil shale to Research, Development, and Demonstration (RD&D) leases instead of commercial leases. Given the many risks surrounding oil shale development, including technological uncertainties, regulatory risks, and water constraints, BLM's proposed RD&D approach makes sense. Investors should be similarly cautious in evaluating future investment in this technology.
New Jobs - Cleaner Air (Part II): An investment in American Businesses and American Jobs
Nov 17, 2011
- In February 2011, Ceres issued a study demonstrating how new air pollution rules proposed for the electric power sector by the Environmental Protection Agency (EPA) will provide long-term economic benefits across much of the United States. This report supplements this economic study by highlighting specific case examples of the companies involved in building a modern generating fleet. It breaks the supply chain into its component pieces and shows the vital role that American workers play in installing and maintaining sophisticated emission control systems.
More Jobs Per Gallon: How Strong Fuel Economy/GHG Standards Will Fuel American Jobs
Jul 30, 2011
- This Ceres report focuses on the economic impacts of strengthening fuel economy and greenhouse gas (GHG) emission standards for passenger vehicles sold in the United States. The analysis finds that stronger standards—more miles and fewer emissions per gallon—would lead to greater economic and job growth, both within the auto industry and in the broader economy as a whole.
New Jobs-Cleaner Air: Employment Effects under Planned Changes to EPA's Air Pollution Rules
Feb 01, 2011
- February 2011 - This study demonstrates how new air pollution rules proposed for the electric power sector by the Environmental Protection Agency (EPA) will provide long-term economic benefits across much of the United States in the form of highly skilled, well paying jobs through infrastructure investment in the nation's generation fleet. Significantly, many of these jobs will be created over the next five years as the United States recovers from its severe economic downturn.
Investor Risks from Development of Oil Shale and Coal-to-Liquids
Dec 01, 2010
- December 2010 - This report shows that coal-to-liquid (CTL) and oil shale technologies face significant environmental and financial obstacles - from water constraints, to technological uncertainties to regulatory and market risks - that pose substantial financial risks for investors involved in such projects.
Canada's Oil Sands: Shrinking Window of Opportunity
May 14, 2010
- May 2010 - This report examines how carbon and land reclamation regulations, climate change and other environmental and social issues may adversely affect the future of oil sands development in Alberta.
Global Climate Disclosure Framework for Oil & Gas Companies
Mar 10, 2010
- March 2010 - In general, companies in the Oil and Gas industry are more transparent than the average of other sectors in respect of their climate-related performances. Nonetheless, the information provided by most companies remains inadequate to fully gauge the exposure of companies to evolving climate change related pressures. This report outlines the main areas of reporting necessary to allow investors to fully assess the impacts of climate-related changes on Oil and Gas companies.
Climate Risk Disclosure in SEC Filings: An Analysis of 10K Reporting by Oil and Gas, Insurance, Coal, Transportation and Electric Power Companies
Jun 10, 2009
- June 2009 - This Ceres/Environmental Defense Fund report evaluates the current state of climate risk disclosure by 100 global companies in five sectors that have a strong stake in preparing for a low carbon future: electric utilities, coal, oil and gas, transportation and insurance. It assesses climate risk disclosure in the SEC filings made by these companies in Q1 2008, and finds very limited disclosure.
CAFE and the U.S. Auto Industry: A Growing Auto Investor Issue, 2012-2020
Oct 06, 2007
- October 2007 - A new analysis by Citi, Ceres and the Investor Network on Climate Risk (INCR) finds that the Senate proposal to raise fuel economy standards for U.S. cars and trucks will have only minor impact on shareholders of auto companies.
The Future of Oil: Energy Security, Climate Risks and Market Opportunities
Jun 06, 2007
- June 2007 - The report from Ceres and the Civil Society Institute highlights key findings from an October 2006 oil analyst briefing at JPMorgan Chase in which Wall Street analysts, institutional investors, and oil industry experts examined how the future of oil will be affected by geopolitics, climate change, and new technologies. The report summarizes the briefing and analyzes several trends that could affect the valuation of oil companies' securities. The report concludes that there are many clean tech investment opportunities that lie ahead as the US government and other governments worldwide move to reduce oil dependence and greenhouse gas emissions.
ExxonMobil's Corporate Governance on Climate Change
May 06, 2006
- May 2006 - Ceres analysis of two new reports from ExxonMobil finds no meaningful advances on climate change, and continued shareholder risks due to lack of leadership on renewable energy.


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It's Pronounced "FUN-KEY FRESH"
OMG! Great photos and amazing blog, i love it!thanks for the comment.i follow you, maybe you follow me back.xxportaltrendy.blogspot.com
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love these items! xx
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very delicious blog))
Love all these pieces!
great pieces!!!
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That outfit is delicious.........love it!!!!
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Thanks the perfect black bra - I have been looking for one and this will be fun.Your collection is quirky and modern - great combinations are what makes something fashion, not just clothes.Violethttp://violetsdayblog.blogspot.com
really great outfit, it's nice to see that someone else is dressing for warm weather!!
Great picks on the pieces! My favorite are the shorts... they are fabulous! LizAnnVPV Internhttp://www.shopVPV.com
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Robert-Jan Derksen carded an eight under par 64 to take lead Johnnie Walker Classic in Perth by a single stroke after the opening round.
The Netherlands' Derksen fired eight birdies in a bogey free round at The Vines Resort & Country Club to finish one shot ahead of Mardan Mamat of Singapore and Australia's Tony Carolan and two in front of the English pair of Lee Westwood and John Bickerton and Ireland's Damien McGrane.
New Zealand amateur leader Danny Lee carded a five under 67 to join last week's winner Anthony Kang, Colin Montgomerie, Ireland's Peter Lawrie, Spain's Ignacio Garrido, Clint Rice of Australia and Hiroyuki Fujita in a tie for sixth.
Derksen managed only one birdie in his first seven holes but the Dutchman reeled off four consecutive birdies from the eighth to the 11th to move to five under.
He missed a three foot birdie putt on the 14th but made up for that by holing a 25 footer at the next to begin a run of three birdies in the final four holes.
The 64 was the lowest ever round for Derksen on The European Tour and helped to alleviate the disappointment of missing the cut at the Maybank Malaysian Open last week.
"It's a good start and it's always nice to have - it's as simple as that - but there's a lot of golf to be played," said Derksen, who has not won on The European Tour since the 2005 Madeira Island Open.
"It's a very difficult course. There are a lot of easy holes and I made three par fives in two, but if you're slightly off, you can make some big numbers.
"The wind is a factor so it's still a long way to go."
Mardan, the 2006 Singapore Masters champion, paid tribute to his caddie Drew Dubberlin after his round which also saw him birdie his last three holes as he finished his opening round just a shot behind Derksen on seven under.
"I called him this week because he has lived here before and knows this course better than I do," said the 41 year old.
"He knows where to put the ball, the pin positions and how to read the lines. There's less of a job for me on the golf course and I can just focus on hitting the golf ball.
"I'm a little stubborn but I do listen to his advice."
Westwood put himself into contention with a strong finish to his round after the World Number 12 struggled initially coming back to birdie four of his last seven holes.
"I started off hitting a couple of poor shots, a couple of pulls and got away with one but I corrected the fault," said Westwood.
"That's what I've been good at doing the last couple of years - correcting things pretty quickly - and I started hitting good shots after that and I played well for the last 12 holes."
World Number 11 Anthony Kim is another in the frame after shooting a four under 68, which the American was happy with after a disappointing opening round in Malaysia last week left him struggling to make the cut.
"It was a lot better, ten shots better - I played a lot better. I found my rhythm, I have got back into the golfing mindset. I made some pretty careless errors out there but good enough to make pars," said The Ryder Cup star.
"My putting wasn't great but it was okay. I'm looking forward to the rest of the week."
Pos | Player name | Nat | Hole | Par | ||||||
1 | LEE, Danny | NZL | 18 | -17 | ||||||
T2 | AGUILAR, Felipe | CHI | 18 | -16 | ||||||
T2 | McGOWAN, Ross | ENG | 18 | -16 | ||||||
T2 | FUJITA, Hiroyuki | JPN | 18 | -16 | ||||||
T5 | JACQUELIN, Raphaël | FRA | 18 | -15 | ||||||
T5 | BICKERTON, John | ENG | 18 | -15 |


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Socks & Hosiery
Sure, bare legs have their moments, but hosiery holds its own as an important part of every woman's wardrobe. Namely, hosiery is ideal for adding dimension and flair, staying warm, and minimizing chafing. Stock your drawers with a complete collection of socks, pantyhose, tights, and leggings. You might need to pay a bit more for quality hosiery that provides more support and is less likely to run or rip.
Kate Spade New York, Cozy tights are, for our money, one of the most stylish ways to keep your legs warm this winter, and this qui...lted pair is extra-pretty. (they even work under pants for super-chilly days. ) stock up!. read more
Featuring silky sheer leg and reinforced toe, our Silk Reflections knee highs from Hanes pamper you with a luxurious fit and feel.... package of 2 pairs reinforced toe nylon/spandex hand wash, line dry made in America one size fits most read more
Our firm control pantyhose provide comfortable shaping so you can wear your most body-conscious styles with confidence. The run-re...sistant, silky sheer legs will provide a flawless finish to your look. comfort waistband shaping panty smoothes tummy and... read more
Flaunt your pedicure this season with the help of Berkshire's toeless sheers. With an ultra sheer leg, you'll feel and look amazin...g all day! read more
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Silky, soft and beautiful, our Silk Reflections pantyhose pamper you with an incredible fit, feel and versatility. control top sil...ky sheer legs reinforced toe nylon/spandex hand wash, line dry imported read more
Check out the NEW 2-Pair Packs of MUK LUKS Fleece-Lined Tights. Our tights are constructed of 95% Polyester 5% Elasthan and have a... 100% Polyester Fleece lining. These MUK LUKS tights make it possible to wear that adorable skirt during the winter... read more
Low Cut, No Show, Heel Grip, Toeless, Boat Socks Men Boat Socks ONLY, other accessories photographed not included. Please check yo...ur measurements to make sure the item fits before ordering For CALIFORNIA residents only: WARNING: This product contains... read more
These Hanes plus pantyhose feature a control top panty and reinforced toe., Women,Accessories,Pantyhose & Stockings
Fiber:50% Cotton. 45% Nylon. 5% Spandex
Mondor 3380 Footed Figure Skating Tights (Suntan) Our durable footed cotton tight is great for everyday practices. It has a sewn e...lastic band foot, comfortable reciprocated toe and the combed cotton softness. read more
No Nonsense Women's Ultra-Sheer Pantyhose give you a flawless finish. They feature a comfortable waistband and super-sheer nylon l...egs. read more
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Trouser Socks for Wider Calves and Feet Get the professional look of trouser socks, created with enough stretch for wider calves a...nd feet. Full calf length socks stretch to fit calves up to 22"and feet up to XXWide width. Non-binding padded tops for... read more
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On the go? On your feet all day? Wear bestselling Hanes Alive full support pantyhose -- and get comfortable support plus sheer bea...uty. Incredibly vibrant, toned feeling lasts throughout your busy day. Silky-sheer knit clings to your curves for sleek... read more
George Lace Top Thigh Hi with Wide Lace Top For Comfortable Fit, Silicone Bands For Extra "Stay Up", Soft Comfortable Leg
90% cotton, 8% spandex, and 2% elastic Featured white Environmental non slip dots on the bottom of the sock.It can give the moveme...nt of the feet provide a buffer and reduce the risk of slipping on smooth surfaces, help you better on the floor or mat... read more
Find your time and experience the ultimate for your run in Avia Ladies' super-lightweight, low cut running tab socks. Composed of ...an all-over mesh for the utmost in breathable comfort, these ultra-light socks eliminate bulk for a snug fit, while the... read more
Womens soft and comfortable fuzzy socks by excell brands
Find your time and experience the ultimate for your run in Avia Ladies' super-lightweight, low cut running tab socks. Composed of ...an all-over mesh for the utmost in breathable comfort, these ultra-light socks eliminate bulk for a snug fit, while the... read more
Womens soft and comfortable fuzzy socks by excell brands
Womens soft and comfortable fuzzy socks by excell brands
No Nonsense 045/333q2 Tan Control Top Nylons
Womens soft and comfortable fuzzy socks by excell brands
Fiber:50% Cotton. 45% Nylon. 5% Spandex
Trouser Socks for Wider Calves and Feet Get the professional look of trouser socks, created with enough stretch for wider calves a...nd feet. Full calf length socks stretch to fit calves up to 22"and feet up to XXWide width. Non-binding padded tops for... read more
A Buster Brown Favorite 100% Soft Cotton Yarn. No elastic or synthetic yarns. Very wide ribbed cuffs, so easy to take on and off w...ithout binding. Oversized to allow for normal shrinkage. Nonallergenic and highly recommended for those with allergies to... read more
74% cotton/ 24% nylon/ 2% spandex Made in USA Machine wash warm, tumble dry low. B. Ellla Nouvella Women's Mule, Slide, Toe Cover ...Socks 74% cotton Made in USA Three Pair Value Pack Varegated, Multi-Stripe read more
Fiber:50% Cotton. 45% Nylon. 5% Spandex
Warm Comfort Knee High Women's Socks
Sofsole Women's Low Quarter Cut Runner Lite Cool Max Socks 6 Pairs White
Great value in convenient 6-pair packs
85% acrylic/ 10% nylon/ 5% spandex Imported Soft and Comfortable Machine wash, no bleach Old School Striped, Retro Tube Style Sock...s Long Live the 70's, Iconic Style Get the Look Without the Bulk. These are THIN, ribbed socks that have the tube sock... read more
Elastic sheer thigh high stockings. Soft and quite comfortable to wear these Stockings. Stretchable so that the Thigh High Stockin...gs can fit most. read more
Great value in convenient 6-pair packs
Fiber:50% Cotton. 45% Nylon. 5% Spandex
These new design Cashmere wool socks are thick and very warm for cold weather. They are perfect chocies for Women who needs warm t...hink socks and have the dress socks look at the same time. read more
Berkshire Ultra Sheer Plus Size Control Top Pantyhose (4411). Enjoy the best of both worlds--firm shaping with a sheer feel--in th...ese pantyhose. Made of nylon and spandex. Ultra-sheer style gives you just a hint of color. Control top pantyhose shapes... read more
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Berkshire Luxury Queen Bottoms Up Control Sheer Pantyhose (5022). Need more than just a little color and help with your waistline?... These super-sheer hose also help give your butt a boost! Made of nylon and spandex. Fine-rib shaping band at top of panty... read more
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Berkshire Ultra Sheers The Skinny No Waistband Pantyhose (5018). Look great in these ultra sheer pantyhose while slimming your tum...my with the control top. Made of nylon/spandex. Non-binding, seamless, stretch waistband for comfort. Control top helps... read more


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The Live Video Annotation plugin allows you to add timed footnotes to a YouTube video. Visitors can see these notes later while watching the video.
folder to the /wp-content/plugins/
directory[videoannotation video_id='VvcohzJvviQ']
in your posts or page. The video_id can be changed to the YouTube ID of the video of your choice.You can adjust the time the note will be visible by adding the expire_time variable to the shortcode.
I you want the note to be visible for 5 seconds, you can use the following shortcode:
[videoannotation video_id='VvcohzJvviQ' expire_time='5']
If you don't want the note to disappear you can set expite_time to 0 (zero):
[videoannotation video_id='VvcohzJvviQ' expire_time='0']
It is also possible to set the dimensions of your video by adding a width and height variable:
[videoannotation video_id='VvcohzJvviQ' expire_time='0' width='500' height='250']
Make sure you are logged in to WordPress and visit the post where you added the video. Start playing the video and type your notes in the field below the video. To submit your note you can press [enter] or click on the annotate button.
Requires: 3.4.2 or higher
Compatible up to: 3.4.2
Last Updated: 4 years ago
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Asus has officially announced its P55 based P7P55D-E Premium motherboard, the first one from Asus that will feature USB 3.0 and SATA 6.0Gbps.
In order to deal with the bottleneck, Asus has included a PLX bridge chip that will give both the USB 3.0 and SATA 6.0Gbps controllers double the bandwidth compared to the plain P55 approach, which means that these two will have higher read and write speeds.
The P7P55D-E has support for Intel's Core i5 and Core i7 processors designed for the LGA 1156 socket, has four DDR3-2200 slots, two PCI-Express x16 slots, six older SATA 3.0Gbps and two SATA 6.0Gbps ports, Gigabit LAN, one eSATA port, 7.1-channel integrated audio and of course two USB 3.0 ports.
For those motherboards that aren't equipped with a PLX chip and onboard SATA 6.0Gbps and USB 3.0 ports, Asus has announced U3S6 expansion card that hooks up to a PCIe x4 slot and features two USB 3.0 and two SATA 6.0Gbps ports. This one is compatible with Maximus III Formula, P7P55D and P7P55D Deluxe/EVO/PRO/LE/LX motherboards as well as probably all other motherboards that can accommodate it.
The motherboard should be available before Christmas, so we're guessing that it should launch by the end of November or early December in the worst case scenario. Unfortunately, the prices for the new motherboard and the U3S6 expansion card weren't announced.
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Asus shows its USB 3.0/SATA 6.0Gbps motherboard
Here comes the P7P55D-E Premium


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Mint Capsule Benefits
Mint capsules are used for a host of medicinal purposes. While mint capsules are also popular as peppermint oil capsules, these are generally more popular than mint extracts or oils because these are easily available, cheaper, and are found to be more effective than the other two.
This blog concentrates on some of the mint capsule benefits:
Aches and Pains
You can swallow a mint capsule for pain because mint has got natural pain relieving properties and various clinical studies suggest that this herb works well for minor pains and aches like headache, etc.
Colon Spasms
Scientists at US National Institute of Health confirmed that peppermint oil capsules effectively tame the spasms in digestive tract. Researchers at NIH say that they have got very strong evidence to prove that peppermint oil can be effective in treating bowel spasms.
As said before the peppermint oil was found effective in treating colon spasms and encourages the production of bile acids; therefore it can be considered as an effective treatment for indigestion and dyspepsia. However, scientists at University of Maryland Medical Center have warned that peppermint oil capsules should not be administered to people suffering from gastrointestinal reflux disease, popularly termed as GERD.
Gas Pain
Researchers at the University of Maryland Medical Center confirmed that peppermint oil capsules can effectively tame the pain caused due to gas in the stomach by easing the colon. The gas passes off easily if the colon is eased. However, the researchers are still looking for more concrete evidence to support this claim. They are hopeful that these capsules can also be used as an anti-gas or carminative digestive aid.
Menstrual Cramps
Peppermint oil capsules are known to curb the muscle spasms in women undergoing menstruation. It has been observed that women who had at least 2 capsules before the onset of menstruation experienced relief from cramps during this period.
Irritable Bowel Syndrome
As said before, the peppermint is revered for its anti-spasmodic properties. Peppermint oil capsules have been found to be effective in lowering the pain associated with irritable bowel syndrome. It is known to curb the intestinal cramping which ensues the IBS.
Regular and systematic usage of peppermint oil is known to curb the formation and growth of gallstones. People suffering from the problem of gallstones have found significant relief in these capsules.
According to Scientist Alan C. Ford, enteric coated peppermint capsules are good for treating diarrhea and their consumption helps to tame the intestinal spasms that lead to diarrhea. Ford found out that peppermint oil was found to be more effective in curbing severity of diarrhea than some well-known fiber supplements and anti spasmodic medications.
By now you might have realized how mint capsules can be utilized to enjoy health benefits in daily life. We suggest you to reach out to these mint capsules whenever you feel some uneasiness in stomach and other small digestive disorders. However, it is highly recommended to seek your doctor's intervention before opting to experiment with its more complicated medicinal uses.
Image Courtesy - apothecacompany.com


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Upstream / Downstream
Explore pathways related to this product.
Important Ordering Details
Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.Santa Cruz discontinued a large number of its polyclonal products as a result of the USDA settlement that was made public May 19th 2016
Find CST Equivalent
To Purchase # 6486S
6486S | 300 µl (3 nmol) | $256.00.0 |
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SignalSilence® Bak siRNA I #6486
Western blot analysis of extracts from OVCAR8 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Bak siRNA I (+), using Bak (D2D3) Rabbit mAb #6947 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Bak (D2D3) Rabbit mAb confirms silencing of Bak expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.
Learn more about how we get our imagesProduct Usage Information
CST recommends transfection with 100 nM Bak siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.Product Description
SignalSilence® Bak siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bak expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).
1. Gross, A. et al. (1999) Genes Dev. 13, 1899-1911.
2. Wei, M.C. et al. (2001) Science 292, 727-730.
3. Zong, W.X. et al. (2001) Genes Dev. 15, 1481-1486.
4. Degenhardt, K. et al. (2002) J. Biol. Chem. 277, 14127-14134.
5. Wei, M.C. et al. (2000) Genes Dev. 14, 2060-2071.
Data Sheets & Documentation
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. SignalSilence is a registered trademark of Cell Signaling Technology, Inc.


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About This Blog
Love Isn't Enough is a blog about parenting and race.
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Tag Archives: school
The first week of school and already a racist incident
By Guest Contributor Renee; originally published at Womanist Musings As much as I love my children, I was absolutely giddy to see them get on the school bus last week. The unhusband and I were singing and dancing, as the … Continue reading
Posted in Race, School, The N Word, racial slurs
Tagged parenting, race, racial-slurs, school, the n word
7 Comments
My son is the only black kid in his class…or is he?
written by Love Isn't Enough columnist Liz Dwyer; originally published at Los Angelista's Guide to the Pursuit of Happiness When I found out I was pregnant for the first time – OK, after taking seven pregnancy tests because I was … Continue reading
Ask ARP: Are we teaching the history of slavery too early?
Dear Anti-Racist Parent, I am a white parent and have two sons (ages 6 and 8). I am committed to doing all I can to raise them in an anti-racist environment. Education is critical to this goal. However, I have … Continue reading
Posted in Uncategorized
Tagged ask-anti-racist-parent, classroom, race, racism, school, slavery, teachers
25 Comments
When saying nothing is the best you can do
by Anti-Racist Parent columnist Tami Winfrey Harris When my husband and I began making plans for my 13-year-old stepson to move from Chicago to live with us in our new home in central Indiana, I was ambivalent. I promise I … Continue reading
Posted in Uncategorized
Tagged african american, black, classroom, discrimination, diversity, family, parenting, race, racism, school, stepmother
3 Comments
Black History Month, Revisited
I wrote this piece a few years ago when I was a monthly columnist for a parenting-related site sponsored by a child advocacy organization. Disclaimer: I really enjoyed writing for that site, and my editor there was the best. However, … Continue reading
Posted in Uncategorized
Tagged african american, black, classroom, culture, diversity, education, history, parenting, race, race-relations, racism, school, teachers
27 Comments
Ask ARP: How to be a strong ally to kids and parents of color at school?
Dear Anti-Racist Parent, My child just started kindergarten at a publicly funded, open enrollment, community operated charter school. We live in a very racially diverse city, and the school is located in a neighborhood that is historically African American, though … Continue reading
Posted in Uncategorized
Tagged ask-anti-racist-parent, classroom, diversity, education, kindergarten, parenting, race, racism, school, teachers, toddlers, white
12 Comments
Ask ARP: What should I do about a conversation about skintone that misses the point?
Dear Anti-Racist Parent, My daughter's preschool teacher had asked me last month if we had any preference for classroom books on family diversity, and I said I had no preference there, since she expressed no two-mom-related issues. But that she'd … Continue reading
Not black enough
by guest contributor Tami Winfrey Harris A couple of months ago, I asked my stepdaughter about a young, black student at her school. "She's okay. You know she talks like a white girl. At first I thought she was weird," … Continue reading
Posted in Uncategorized
Tagged Uncategorized, african american, black, parenting, race, school, stereotypes
29 Comments
Ask ARP: How should teachers handle books containing racial slurs?
Dear Anti-Racist Parent, My neighbors and I all have boys in the 3rd grade. A few weeks ago, their teacher was reading a story that included the n-word which prompted the kids to ask "what's that?". The teacher gave a … Continue reading
Who Belongs?
by Anti-Racist Parent columnist Natasha Sky My son was writing in his workbook the other day. We're homeschooling in the eclectic-semi-unschooling-style; the workbooks have been around for over a year–my kids think they're fun. The books are actually a great … Continue reading
Ask ARP: What should I do about nursery rhymes with a racist past?
Dear Anti-Racist Parent, I am a Mom of two girls and I live in Eastern Canada. I am looking for some advice about the children's rhyme "Eeny, meeny, miny, moe, Catch a tiger by the toe". Last week, on the … Continue reading
A Slur By Any Other Name
by Anti-Racist Parent columnist Paula, originally published at Heart, Mind and Seoul When I was in the 2nd grade, my teacher would occasionally ask for a student to volunteer and help close the end of the school day with a … Continue reading
Posted in Uncategorized
Tagged Uncategorized, classroom, friendship, race, racism, school, teachers
13 Comments
Parent Panic Over Hispanic Heritage Month
by Anti-Racist Parent columnist Maegan "la Mala" Ortiz Maybe it's because I'm a conscious Latina parent with a background in activism. Maybe it's because I hate and don't identify with the term Hispanic. Whatever the reason, I don't celebrate or … Continue reading
Tagged Hispanic, Latino, Uncategorized, classroom, culture, parenting, school, teachers
5 Comments
Inviting me to the party
by Anti-Racist Parent Columnist Jae Ran Kim, originally published at Harlow's Monkey This past Friday was our school's annual Fall Picnic. As you may recall, last spring I wrote about being snubbed from a Mom's Night Out event in which … Continue reading
Tagged Uncategorized, diversity, friendship, parenting, pta, school, teachers
13 Comments
Ask ARP: What resources on adoption can I give to my daughter's pre-school teacher?
Dear Anti-Racist Parent, I'm looking for some resources on talking about race, adoption, and transracial adoption that I can pass on to my daughter's preschool teacher. I've googled a variety of things but haven't had much luck. Anything anyone can … Continue reading
Helping teachers understand adoption
by Anti-Racist Parent columnist Margie Perscheid, originally published at Third Mom My approach was to keep it simple – both because as a former teacher I knew that my children's teachers would have limited time to spend reading the packet, … Continue reading
Columnist Intro: Maegan
by Anti-Racist Parent columnist Maegan "la Mala" Ortiz My parents, who came to New York City from Puerto Rico as teenagers, were not anti-racists. How else to explain how I, their first born, was named after the priest seducing white … Continue reading
Tagged Hispanic, Latino, Uncategorized, colorism, columnist intro, discrimination, education, language, parenting, pregnancy, race, racism, school, teachers
11 Comments
If I could go back to the classroom…(part 3/the conclusion)
by guest contributor Jen Chau, originally published at The Time Is Always Right… Here's the conclusion: Flash 1: Our date with destiny. I mean…the principal. Conclusion: Paper cutting, Cave paintings, Jackson Pollack splotches, and quizzes. Moral: Think creatively! I had … Continue reading
Tagged Uncategorized, classroom, education, race, school, teachers
2 Comments
If I could go back…to the classroom (part 2)
by guest contributor Jen Chau, originally published at The Time Is Always Right… Not even the most well-designed and sparkly lunchbox could have emotionally prepared me for my first week of school. I guess what works as a kid doesn't … Continue reading
Tagged Uncategorized, classroom, education, race, school, teachers
7 Comments
If I could go back…to the classroom
by guest contributor Jen Chau, originally published at The Time Is Always Right… [dedicated to V and Crane] If I could go back in time to my days as a teacher, I would do things very differently. Isn't it easy … Continue reading
Tagged Uncategorized, classroom, discipline, education, race, racism, school, teachers
5 Comments


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LHC Offline Until April 2009 (Or Longer) 298
rufey writes "The recent problems at the Large Hadron Collider will now keep it idle until spring 2009. The official press release is here. The LHC went offline due to a suspected failure in a superconducting connection, which overheated and caused around 100 of the LHC's super-cooled magnets to heat up by as much as 100 degrees. This resulted in the accidental release of a ton of liquid helium. The process required to repair the failed superconducting connection involves weeks of warming up the affected area from -456 degrees Fahrenheit to room temperature, and then several more weeks to cool it back down after the repair is made. The total amount of time to do this will spill over into CERN's scheduled winter maintenance/shutdown period, which is partly done to save money on electricity during the period of peak demand."


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dermalogica ChromaWhite TRx Brightening Regimen
This kit delivers brighter, more luminous skin while helping to control hyperpigmentation for a more even skin tone. Contains Tri-Active Cleanse (tone-improving lather), Powerfoliant2 (potent brightening exfoliant), C-12 Concentrate (clarity-enhancing serum), Pure Light SPF30 (daytime treatment protection), Pure Night (overnight treatment moisturizer).
How to Use: Lather Tri-Active Cleanse in wet hands and apply to dampened face and throat. Avoid the eye area. Rinse thoroughly with warm water. Apply C-12 Concentrate directly onto areas of discoloration. Allow to absorb. Next, dispense a small amount of Extreme C onto fingertip and apply by smoothing directly over areas where pigmentation is prominent. For all-over brightening effects, mix a pea-sized amount into Pure Light SPF30. Apply Pure Light SPF30 to face and neck with light, upward strokes.
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Jason M. DaPonte has been promoted to supervisor at Sansiveri, Kimball & Co. LLP, where he counsels emerging and private businesses, and construction clients. DaPonte holds an M.A. in accounting from the University of Rhode Island and a B.S. in business from Providence College. •
Tuesday, March 21
4 days ago


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Why Malays are gravitating towards DAP
The platform from which our voices can be heard and opinions shared is far better than the one Umno Baru may provide, says Syerleena Abdul Rashid.
There is a lot of speculation as to why DAP decided to field 'newcomer' Dyana Sofya Mohd Daud as its candidate in Teluk Intan.
To most she is seen as a protégé, while those who are still unable to fathom her decision to join DAP have begun their personal campaign to destroy her image and credibility.
As a matter of fact, any Malay candidate would have been subjected to certain levels of political animosity and slander. In the past, other Malay candidates had gone through the same maltreatment and been imperilled by vicious threats made by ultra-right wing hate groups.
Being relatively young (compared to the average age of our MPs) and beautiful (though beauty is subjective) makes her an easy target, but the naysayers have underestimated this intelligent lass. Dyana Sofya is the face of hope and will bring forth much needed change to this country.
Of course, at this stage, Malaysians are aware of how vicious the powers that be can be when they have an agenda to accomplish, and these past few weeks leading up to her nomination showed us again just how uncouth they are capable of being.
For decades, the DAP has been labelled a 'Chinese party' and accused of being chauvinistic. It is undeniable that Malaysians of Chinese descent make up the majority of party members, but that is merely coincidental as a direct result of Umno's Malay-centric policies.
Some might even say that years of disseminating misinformation via government-controlled media influenced our political views and distorted our logic. But the truth is in the name and there is a reason why DAP stands for Democratic Action Party.
When Umno Baru and its supporters spew bigoted slogans, it only confirms it is utterly oblivious towards its own homogenous brand of race-based and raciallybiased policies, which have been etched through their own party names (Umno Baru, MIC & MCA).
The local political scene, before alternative media were available to average Malaysians, was far simpler. It was 'us' versus 'them', and this tactic worked for a while for Umno Baru. But like everything else that lacks maintenance and care, or in this case, when a party has become too complacent, slowly but surely, cracks begin to appear.
Many of us see flaws in the system; we know how policies and funds were misused; we know of the business opportunities that lay ahead of us if we pledge our loyalty to them.
We see things in a different way now and we want to progress as a nation – together. We are tired of being segregated because of our race and religion. We do not need to be told whom we are allowed to befriend and support.
We are worn out by the lies; our education system is deteriorating, and the economy is not doing so well. The powers that be seem out of touch with the harsh realities Malaysians have to deal with every single day. They justify the need for GST and the recent increase in electricity tariffs, but these are fabrications that the powers that be churn out haphazardly; hence, the never-ending flip- flop in published statements and remarks.
But the concerns of the minorities and the marginalised are different as most of them have become victims of developmental promises gone awry. This is one of the many issues that must be given priority.
Unfortunately, the powers that be have lost the plot. They are too busy enriching their own lives and living comfortably in their politically incestuous circle.
When Malaysians of Malay-Muslim descent join DAP, we are harshly marked as apostates, traitors and unappreciative of the privileges the Constitution has guaranteed us. The truth could not be further, and questioning our loyalty is unreasonable. The Constitution does not favour any political party but favours the rights of all Malaysians.
Our system does not demand Malaysians to blindly accept any specific political doctrines, nor does it require us to brand each other as ingrates. We must be made to understand that loyalty is not conformity, and true loyalty does not command the destruction or blocking of another person's loyalty.
Loyalty is a faithful adherence which involves a bond between people or allegiance to a cause or principle. It is not a mere sentiment or opinion.
But understand that loyalty is by no means mechanical obedience. Malaysians are permitted to adhere freely and firmly to what they value as respectable and genuine, not to what is imposed or forced upon them.
We have become too indoctrinated with political loyalties that we have forgotten human fidelity, and this is the message we want to get across. The conflicts sandwiched between religious loyalty and political loyalty have been too frequently dramatised and over sensationalised by the powers that be.
Many of us join DAP (and Pakatan Rakyat) because we see the opportunity to contribute to the development and progress of our great nation. The platform from which our voices can be heard and opinions shared is far better than the one Umno Baru may provide.
The former is now seen as a catalyst for change and they are willing to listen. The latter has become too synonymous with the distressing culture of corruption and has stopped listening to Malaysians some time ago.
We know that the powers that be will shower marginalised communities with one-off payments and boast about how they care for the well being of these communities, but that does not amount to sustainable development. I am a firm believer of the saying, "Give a person a fish, he will eat for a day. Teach a person to fish, she will eat for a lifetime."
This approach to development and progress will have greater impact in empowering fellow Malaysians, and that will be the key factor that can see through our country's transformation to a developed nation. And this will not require us to cheat and steal RM250m from those who rightfully need it.
Yes, most of them come from families who have supported Umno one way or another; some of us come from families that still do.
But we also know of the heartaches Malaysian families have gone through because they were not well connected enough to get into public universities or to obtain much-needed financial aid. These issues are enough to make us speak out against the oppression and discrimination our fellow Malaysians are subjected to.
Fact is, many of us see renewed hope, optimism and confidence in people like Dyana Sofya, Senator Ariffin Omar, Zairil Khir Johari and Mohd Ariff Sabri Abdul Aziz. This is something many of us find extremely inspiring.
But, on a lighter note, for these politicians and other DAP members of Malay-Muslim descent, this coming Raya family gathering will definitely be very interesting and 'lively'.
Syerleena Abdul Rashid is the political education director of the Bukit Bendera DAP women's wing.
Im enjoying this. More and more Malays for DAP.
Well said. Cheers! Syerleena!
In our modern globalized society with free flow of information, UMNO has lost its appeal among many informed Malays who can see through their hypocrisy. Sometimes I can't help but wonder what is in the minds of these Malays when they read or hear about 'the advice of not becoming a traitor of her race' given by the likes of Sharizat of the NFC fame to the DAP candidate (Dyana). In my opinion, she should save the advice for her own family and impart her advice to others only after her family has shown remorse by repaying the rakyat's monies. But strangely enough, she just did not realize that she is living in a glass house.
You have to start from somewhere and in the case of DAP it was started unfortunately by this mad … LKS. It did not mean that it is n alternative to MCA and no doubt DAP has come a long way. It is beginning to be a threat, not only to the survival of MCA, but most importantly it is beginning to become an alternative political party for the well educated Malays to be involved in politics. It is an untainted political party yet to be involved with and that really scares the hell out of all the Umno schmucks.
As a consequent the schmucks have to turn to the only thing left and that is the religion it is supposed to represent. Plus they have also the right to be violent, threat to commit arson and all sorts of things these schmucks can think of and act on. I might even add this, without any impunity by the police. Can anyone else openly threaten to commit arson and still able to get away from it ?
What further proof do you need that these Umno schmucks can get away with anything without any punitive action taken against them by the police.Thus these schmucks in Umno are getting bolder and bolder by the day no doubt what kind consequences it will be.Najib, have you ever heard of spare the rot and spoil the child ? Malaysia will one day soon become a nation full of criminals and terrorists just like that of many countries. You are supposed to build a nation just like what a good father should do to bring up his children. But because of their votes for you to stay in power you are doing just the opposite. I dread to think what Malaysia will become with you as the PM and all the Umno schmucks have be doing ?
… are you trying to turn Malaysia into another Afghanistan and Pakistan …(?)


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Shadertoy Multipass Demopack for GeeXLab
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Mali GPU Offline Shader Compiler UpdateWe are please to announce the latest release of the Mali GPU Offline Shader Compiler v4.0.0.The latest release adds support for the Mali-T604 and Mali-T658 family of GPUs from ARM. The Mali-400 support has also been updated to include support for the latest driver version (r3p1-01rel1)


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Glossary of Terms
Diagnosis Terms
Professional Term | Meaning |
Adrenal glands | Two small, triangle-shaped glands, one located on top of each kidney. These glands secrete various hormones, including androgens |
Benign | No cancerous and generally not life threatening |
Biopsy | A test that involves the removal of cells or tissue for examination under a microscope |
Cancer | A term for diseases in which abnormal cells divide without control and possibly spread |
Digital rectal examination (DRE) | An exam in which a doctor inserts a lubricated, gloved finger into the rectum to check for anything abnormal about the prostate |
Hormone | A chemical in the body produced by glands. Hormones circulate in the blood and control how certain cells and organs work |
Malignant | A cancerous tumor that can grow and spread and may be life threatening |
Metastasis | The spread of cancer from one part of the body to another part |
Pituitary gland | A gland located at the base of the brain. This gland produces hormones that tell the testes and other organs to release other hormones |
Prostate | A walnut-sized gland in men that surrounds part of the urethra. It is located below the bladder and in front of the rectum |
Prostate-specific antigen (PSA) | A substance in the blood that often increases in cases of prostate cancer and other prostate conditions |
Scrotum | The outside pouch that contains the testicles |
Stage | The size and extent to which the cancer may have spread |
Testes | Male reproductive glands that produce sperm and testosterone |
Testosterone | The male hormone responsible for the sexual characteristics of men |
Tumor | An abnormal mass of tissue that results from uncontrolled cell division and growth |
Urethra | The tube that carries urine from the bladder and semen from the sex glands |
Treatment Terms
Professional Term | Meaning |
Adjuvant hormonal therapy | Therapy that is given immediately after surgery or radiation in order to destroy cancer cells that may remain anywhere in the body after these procedures |
Androgens | Hormones that produce the sexual physical characteristics of men (such as deep voice and facial hair). The main male androgen is testosterone |
Antiandrogens | Drugs that fight prostate cancer by blocking the action of testosterone. These drugs are given as a pill |
Bilateral orchiectomy | Surgical removal of both testes, which results in castration |
Biological therapy | Treatment to stimulate or restore the ability of the immune system to fight infection and disease |
Brachytherapy | A procedure in which tiny "seeds" made up of radioactive material are placed directly into the prostate |
Castration | Treatment that suppresses testosterone. Castration can be achieved surgically (orchiectomy) or medically (using an LHRA-A) |
Chemotherapy | Treatment with anticancer drugs that attack the cancer cells but may harm certain types of healthy cells |
Combined hormonal therapy | Combining two types of hormonal therapy-a pill (an antiandrogen) and an injection (an LHRH-A) |
Cryotherapy | Repeated freezing and thawing of tumor cells, which result in cell death |
External beam radiation | Radiation therapy that uses a machine to direct high-energy rays at a certain part of the body where cancer has been detected |
Hormonal therapy | In prostate cancer, treatment that interferes with the production of and block male hormones (such as testosterone) that promote the growth of tumors |
Luteinizing hormone (LH) | A substance produced by the pituitary gland in the brain that stimulates the secretion of sex hormones in men and women |
Luteinizing hormone-releasing hormone | A hormone secreted by a part of the brain (hypothalamus) that trigger the release of LH |
Luteinizing hormone-releasing hormone analog (LHRH-A) | Drugs that treat prostate cancer by keeping the testes from producing testosterone. These drugs are typically given as an injection |
medical oncologist | A doctor who specializes in diagnosing and treating cancer using chemotherapy, hormone therapy, or biological therapy |
Neoadjuvant hormonal therapy | Therapy that is given before surgery or radiation in order to improve the results of these procedures |
Palliative therapy | Therapy that is given to provide symptom relief but is not intended to cure the disease or extend life |
Prognosis | A prediction that is made about the potential outcome of a disease |
Prostatectomy | An operation to remove part or all of the prostate |
Radiation oncologist | A doctor who specializes in using radiation to treat cancer |
Radiation therapy | Treatment for prostate cancer that uses radiation to kill cancer cells and shrink tumors |
Radical prostatectomy | An operation to remove the entire prostate and some of the tissue around it |
Recurrence | When a cancer returns a the same site as the original tumor or another location, after it had disappeared |
Systemic therapy | Treatment using medicines that travel through the blood to reach cells throughout the body |
Urologist | A doctor who specializes in diseases of the urinary and sex organ in males and the urinary organs in females |
Watchful waiting | Closely monitoring a patient's condition without treatment, using regular digital rectal exams and PSA blood tests; also called observation or surveillance |
Side Effect Terms
Professional Term | Meaning |
Gynecomastia | Enlargement of the breasts in males |
Hot Flashes | Sudden rushes of body heat |
Impotent | Not able to have an erection adequate for sexual intercourse |
Urinary incontinence | Not able to control the flow of urine from the bladder |


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GREENLAND – Robin Bettencourt of Robinwoods Design is teaming up with Rolling Green Nursery again for a Winter Artisan Market on Saturday February 9 from 10am – 4pm.
The fair will take place at Rolling Green's Greenland location on Breakfast Hill Road -- in their brand new glass greenhouse, a beautiful, serene venue for browsing and shopping.
Vendors participating at this market include Robinwoods Design, Felt Sutra, Funky Rock Designs and Hoist Away Bags among many others. In celebration of Valentine's Day there will be gourmet chocolates, baked items, wine and cheeses for sale. The public is welcome and there is plenty of parking. Please call 436.2732 or visit rollinggreennursery.com for more information.
Rolling Green Nursery is a nine-acre grower and retail garden center.
committed to raising healthy and vigorous plant materials for your projects. They provide expert horticultural advice throughout southern New Hampshire. From annuals, perennials and flowering shrubs to ornamental trees and native selections, Rolling Green provides quality plants to enhance your landscape with color, texture and beauty. Learn more at rollinggreennursery.com.
Artisan Market planned Saturday at Rolling Green Nursery facility
Feb 8, 2013 at 3:15 AM


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My Account Sign in
Chopper, our 95 lb. yellow lab, swims every day in our AZ heat. After having an ear infection a few months ago, his ears stay clean and dry with this after-swim treatment. Love it!
Just pour this solution in your dogs ears, they shake their heads and it cleans them instantly. Quick drying and with a fresh and claen smell makes this the best ear solution ever. My dogs never itch their ears after I use it. Highly recommend it.
This ear cleaner does a very good job. Also, easy ordering process and fast shipping.
I have had 5 hound dogs over the course of the past 25 years and their ears always need attention. This cleaner keeps down the yeast infections by controling moisture and smells better than all the rest! You can pay more however this is the BEST cleaner/moisture control product out there of all the products we have tried..
Love this product~ This keeps the ears in healthy condition~
My first yorkie suffered for years with ear infections. At 10 years old, a new vet suggested this product. He never had another ear infection for his remaining six years. I've used this product on my current yorkie (15 years old) starting with his first bath as a pup, and he has never had an ear infection. His vets have always commented on his clean ears.
Had to do a little bit of searching to find once our vet stopped carrying this brand. Well worth the hunt. Will definitely purchase again.
My bloodhound has allergies which has caused ear issues. I started cleaning her ears daily with this solution and now I only clean them once every week or two and no ear issues. Its gentle and smells great. I tried other products but they only made her ears red and irritated. This is a great product for every day or just when needed. Great price too !
The scent of this product is (to me) awful, and so strong that I was unable to get it off my own hands even after repeated washings. I dread to think how long my dog's ears are going to smell like this. I don't recommend it.
Our vet recommended this product. We use it once a week on our spaniel to eliminate ear infections. I needed to replace this but the vet was charging twice as much as EntirelyPets.com. Also, I like the free / inexpensive samples and quick shipping.
Very effective product. Priced right too
Great Product and Received quickly... Thanks
Our German Shepherd has a tendancy towards yeast infections in her ears, the vet recommended this product to maintain her ears. It's safe enough to use daily, but we use it weekly. I would recommend anyone to use this for their pets. Also, the scent is NOT offensive...a little strong, but that is just one thing you have to put up with because every other med is the same!
The only brand my vet uses. I tried other brands to compare...there's no comparison. This solution breaks down the "gunk" in my dogs ears leaving them smelling clean, not medicinal. Takes care of ear infections due to yeast and, with regular use, helps keep them from coming back.
Have used this product for years. Easy to use and really helps get ears clean.
My English Bulldog is prone to ear infections. When we use this product, his ears are great. If we stop using it or switch brands, his ears are red and itchy again. This cleanser also has a nice clean smell.
The best and has a wonderful fragrance that is not offensive
I love this product, I have tried several other ear cleaning products, and have always come back to this one, it smells really good to, not only that it is really reasonable priced when buying online, my vet sells this product for 10$ higher then i pay for it online, thanks for keeping the price down.. It works really well to!
I use this routinely to clean our dogs' ears. It's effective, doesn't sting, has a pleasant scent, and nips a lot of ear issues in the bud.
I had used this product before from my vet.Then they stopped selling it and what they replaced it with didn't seem to do the job this Product does.
Cleans ears completely and leaves ears fesh smelling!
I have pet bunnies. This solution works well for period cleaning of their ears. I also have a tabby cat, and I use this on his ears every couple of weeks. He is an outdoor cat in a humid climate, and it works very well.
My vet has been selling me this product for my dogs for years. I just discovered it is less than half the cost for twice the size!!! It's a wonderful ear cleaner.
I love this ear cleansing solution. Works great, keeps ears clean and has a pleasant smell. Would highly recommend this product.
I find this ear cleaner the best we have found so far. It does not sting, has a pleasant smell and works to keep Buddy's ears clean.
As a groomer I use this product daily on every pet that comes in....simple to use and I find very few ear infections with pets that use this on a regular basis. Awesome!!!
I have English Springer Spaniels. They tend to get ear infections due to the hanging down ears and the humidity where we live. I have used this product for years and think it is the best on the market. Unfortunately my vet stopped carrying it when he sold out to VCA. So now I buy it over the Internet,
I've been using Vet ear cleansing solution for over 8 years and none of my 3 dogs have had an ear infection. It smells nice too!
I had tried several products our vet recommended for our lab who swims everyday. After ear infections every couple weeks, I decided to go back to this product which I used on our other lab. Works great, and drys the ears out completely! Since using this product, our lab has not had anymore ear infections!!
This is great for keeping problem ears not a problem.. Dogs with long ears, like our CockaPoo, tend to have sore ears and sometimes smelly issues, this keeps them clean and her ears don't need a doctor to keep them clear of infection. And like most Vet Solutions products it smells great.
Over the years, I've used many ear cleansing products on my German Shepherds, with this being the best. I never pour it directly in the ear -- I always squeeze it onto extra-large cotton balls and take a little time to carefully swab all areas of the ear and gently get as far down as I can. (It takes about 4 cotton balls per ear when I do a really good job if the ear is a little dirty.) I use it once every 2-3 weeks or so, more if their ears have been dirty. It's also good to do if they've gotten their ears wet (after a rain, after a bath or swim or been playing with the hose outside) to make sure their ear canals stay clean and dry. My dogs don't mind this gentle attention, the older ones even seem to like it. I always give a treat afterward. For dogs that don't like to have their ears cleaned, you can wait until they're calm (after exercise) and then feed them small pieces of treats while you're doing it (works best with a partner) -- this method works great for nail trimming too.
This product was recommended to us by friends for our springer spaniel. It was the only product he didn't fight me on while cleaning his ears. I think the other products burned.
I have a Cocker Spaniel and those big floppy ears get cleaned twice a week. When I got him he was 3 y/o and had double perforated ear drums. Lots of treatment later his ears are healthy, and it's imperative I keep them clean. This product works great - I've been using it for over 2 years.
Great for use after swimming. Keeps the dogs from getting swimmer's ear.
It works to clear up a yeast infection along with antibiotics when used regularly. It does have a strong odor, but is not terribly offensive
The ear solution was sent out to us twice. It leaked from the bottle and what a mess in the postage bag and everything else. I explained that to the company and they said they would make sure it arrived taped. Yeah it was taped but the design of the lid causes it to leak out on shipping. They need to rethink packaging.
I use this product on my dogs after their baths every other week and in between baths to keep their ears smelling clean and fresh. My dogs never have any of that brown goop buildup and don't get ear infections.
I have been using this for years. I wouldn't wash my dogs without it. Put some in their ears when they're getting bathed and have never had an ear problem. Perfectly nice odor also.
It is good to clean out dogs ears. Gentle for the ears.
i have four bassets in my home and so keeping ears clean and healthy and smelling good is a full time job. i love this cleaner. it is so gentle and smells great. my female basset maggie may has very tender skin in her ears and this doesnt make it red like many clearers i have tired.
This is the best ear cleaner for my Poodles! It keeps their ears clean with regular use. And it is the only ear solution I have tried that they don't abhor!
Our Lab mix has the characteristic "floppy" (cute) ears that encourages yeast to grow. This solution has a nice fragrance and is effective in creating an environment that minimizes the brown, yeast build-up. "A MUST" for weekly, if not more often, maintenance. Great price for 16oz. -- almost the same price as the 8 oz. size I can obtain locally.
Have purchased this at vet for a lot more, with 2 golden retrievers, i use it for cleaning and refreshing all year, best plus it has a great smell. not that good for an established ear infection but great product.
I have had Rhodesian Ridgebacks for 25 years and love them! I use Vet Solution Ear Cleansing Solution weekly - its keeps their ears clean and healthy and smells great.
Everyone I know use this product to clean their dog's ear.
My vet told me I should routinely clean inside the ears of my two basset hounds. He told me that I could use cotton balls and a mixture of 1/3 white vinegar, 1/3 hydrogen peroxide, and 1/3 water. That solution worked but smelled bad and my dogs didn't like it. I switched to buying and using Vet Solutions Ear Cleaning Solution, and my dogs don't dread the cleaning procedure. The inside of their ears are clean, smooth, and actually smell good.
works well..does what it says on the bottle
I have been using Vet Solutions ear cleansing solution on my Shih Tsu since she was a puppy and she has just turned 10 years old this month. It is simple to use and the scent is a very clean right out of the shower scent. I use it once a week and she has never had an ear infection or even yeast build-up. Her vet always says "Lily's ears look so pink and clean". Needless to say my dog loves it and so do I!!!
I've been using this ear cleaner for many years, it is excellent and smells good too! easy to use, even every day if needed.
I have been using this ear cleansing solution for 15 years and it is great. It prevents ear infections when used as directed. It is wonderful and worth every penny. I would not have a dog without it. do yourself and your dog a favor. buy and use this product regularly. you will not be disappointed.
This product helps eliminate the discomfort which causes ear scratching and ear odor. Easy to use and pleasant smelling. Good for dogs and cats.
My 13 year old Lab loves to swim but he is prone to ear infections. This cleansing solution works wonders by preventing that nasty, smelly and irritating infection that only antibiotics can heal. I have been using this solution for many years and it leaves my Lab's ears clean and odour free. It even leaves a pleasant fragrance in his ears.
This is the same solution my Vet uses in her office and recommends I use for my Cocker Spaniel's ears. I use it every day to clean her ears in conjunction with an internal solution and it works like a charm. It is non-burning and is very soothing.
I have 2 dogs that are prone to ear infections. This cleaner works wonderful.It does smell different than some of the other cleaners, but you get use to it. The price on this site is very reasonable also, my vet charges alot more for it.
I usually buy ear cleaner from my vet. My Jack Russell has numerous allergies that effect her ears. The Cleansing solution soothes them so I use a lot of it! I pay $18 for 8 oz at the vet's office. At Entirely Pets I got 32 oz for just about the same price! HUGE savings!! Thank you Entirely Pets!
I have Cocker Spaniels that tend to have problematic ears. I have tried many ear cleaning products and this one is definitely the best. It is gentle enough to use frequently when problems arise and is great for routine cleaning. My pups have had less problems since we started using this a few years ago at the vets recommendation. I use the solution after bathing or swimming to alleviate "swimmers ear" and clean ears keep yeast infections at bay. Highly recommended.
Love this product. I have a dog that swims everyday in the summer and has floppy ears so he is very susceptible to ear infections. This product is gentle enough to use everyday and helps to keep my dog's ears clean. I got this product the first time from the vet and now I buy it cheaper from EntirelyPets.com
Have a dog with floppy ears? Spending too much money on the stuff your vet wants to sell you to care for them? This works great and you get more bang for your buck! Squirt it in, rub on your dogs ear (hear it swishing around inside) and dry it out with a cotton ball or paper towel....done!
My cocker spaniel has chronic ear problems, so it's important that I clean them regularly. This product is great. Does the job well, smells decent and as much as my dog hates having his ears clean, it helps clear up and prevent infections.
Has been working very well for my dogs. Good price too!
I have use this product for years it works ! on both the dog and cat deodorizes and it smells clean.
We have been using Vet Ear Cleaning Solution on our Bulldog for two years. Prior to that we had problems with ear infections, but since using the product along with regular cleanings we have had none. We also use it in his tail pocket, it acidifies so it stops the bacteria that grows in the pocket. For us this product has saved us $$$$$$
Excellent service.
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,,[],, *******,,[],,
A Command and General Staff College student and an Army Reserve Soldier demonstrated their martial arts prowess at a recent sporting exhibition as part of an Army recruiting event.CGSC student Maj. Rob Pickel and 1st. Lt. Joe Proctor, of the 8th U.S. Army, were two of 14 Soldiers to fight in the GIs versus Joes II combative sports event Feb. 22 at the Saint Charles Family Arena near St. Louis.Army Recruiting Battalion St. Louis partnered with Kick International to sponsor the event, which drew about 3,000 people, said an event organizer. A portion of the proceeds will be donated to the Army Emergency Relief fund.The Soldiers did well winning six of the 14 matches, which were divided by weight class, said Pickel, who won his fight in a three-round decision.Mixed martial arts is similar to what is used in the Ultimate Fighting Championships, said Pickel, a Special Forces officer.
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The Presence of Wonder
27 comments:
- June said...
I'm sure the cardinals, et al are so happy to have your feeders available! It's incredible, the weather SW Virginia is having this year!
- February 3, 2010 at 6:49 AM
- Kelly said...
...your snow queen is beautiful. The white of the softly falling snow and the green of the pine is perfect to her browns and reds. Love this shot...and that of the dripping and melting snow on the pine branch. Hope your storm passes you on by. I hate the ice storms too. They do so much damage to the trees.
- February 3, 2010 at 6:50 AM
- Denise said...
Absolutely gorgeous photos! I do love your little cardinal. I live in Northern Virginia 25 miles out of Washington DC. That was quite a snow we had last night. I don't think I will be going anywhere for a while.
- February 3, 2010 at 7:24 AM
- Steve Borichevsky said...
VE it! Nice shooting! - February 3, 2010 at 7:27 AM
- mountain.mama said...
Great pictures, you are the Queen of bird shots!
- February 3, 2010 at 9:17 AM
- Snappy Di said...
Such a great looking bird... Love cardinals. I was out for a walk two days ago and saw one in a tree off the side of the road... unfortunately I had the wrong lens on my camera and off he flew. Next time my walk will include BOTH camera lenses.
The Blue Ridge Gal - February 3, 2010 at 9:41 AM
- Gayle said...
Robert Crais is a fave, one summer I read all of his, called it my Craisy summer. :)
Huge fan of M. Connelly, H. Coben, D. Lehane, Grafton, and all of your birdie pics. My fluffy, windblown cardinal is such a hit!
Gayle - February 3, 2010 at 11:06 AM
- Happyone :-) said...
Always love your photos.
I like all the Sue Grafton mysteries. - February 3, 2010 at 11:11 AM
- Chris said...
This is a very nice picture of this nice bird... you still have snow, we almsot did not get any over here! - February 3, 2010 at 12:11 PM
- Ruth's Photo Blog said...
Beautiful pictures.The pine needles are exceptional.Stay safe during the next storm.
Blessings,Ruth - February 3, 2010 at 1:59 PM
- A New England Life said...
Kerri, I am so impressed by that beautiful shot! You have to tell me how you captured her so perfectly. She really does look like a snow Queen!
Enjoy the snow ; ) - February 3, 2010 at 3:38 PM
- dAwN said...
Gorgeous Cardinal photo...looks beautiful in the snow..
- February 3, 2010 at 4:17 PM
- JO said...
nice! I love all your pictures.
Mine's up. - February 3, 2010 at 4:50 PM
- cindyzlogic said...
Gorgeous photos, Kerri!! I really enjoy your work!
- February 3, 2010 at 5:18 PM
- Barb said...
Sometimes, I just sit and stare at you photos for awhile! They bring me such peace. I hope the ice passes you by - we actually NEED snow in the mountains of CO.
- February 3, 2010 at 5:52 PM
- imac said...
I love the macro shot of the grass and water drops.
- February 3, 2010 at 6:00 PM
- eileeninmd said...
Awesome shot of the Cardinal, Kerri. She is so pretty.
- February 3, 2010 at 6:19 PM
- eileeninmd said...
Awesome shot of the Cardinal, Kerri. She is so pretty.
- February 3, 2010 at 6:19 PM
- Country Girl said...
Beautiful shot of the female cardinal, one of my favorite wintertime birds. I love those rich colors.
- February 3, 2010 at 8:27 PM
- Hilary said...
She's a beauty, alright. Just gorgeous.
Thanks for the linkage. :) - February 3, 2010 at 9:54 PM
- Mary said...
Really beautiful shot of Mrs. Cardinal in the snowy pine!
- February 3, 2010 at 11:00 PM
- Kathy said...
Absolutely stunning. I live in AZ where I've never seen a cardinal, although I grew up with them in the South. Thanks for the memories.
- February 3, 2010 at 11:51 PM
- Carletta said...
She is a beauty! Her coloring with the green and white is lovely!
The drippy pine needles are just awesome as well. - February 3, 2010 at 11:56 PM
- Dick said...
Really beautiful pics. , wonderful bird I like the second picture the best because it's so pure and simple.
- February 4, 2010 at 1:08 PM
- forestal said...
Wonderful photo, Great contrast with the pine
dan - February 5, 2010 at 6:16 PM
- CG said...
Those cardinals are so beautiful - how could we not be grateful for a world with such amazing creations in it? I hope you and your birds are surviving the snow!
- February 8, 2010 at 9:50 AM
- Reena Walkling said...
They add so much beautiful color during winter!
- March 3, 2014 at 12:50 PM


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Final ex-Vanderbilt player turns himself into cops
NASHVILLE, Tenn. — Brandon Banks has turned himself into police, the fourth and final former Vanderbilt player arrested after being indicted on five counts of aggravated rape and two counts of aggravated sexual battery involving an unconscious student at a campus dormitory in June.
Police spokesman Don Aaron said Banks turned himself in Sunday night, and bond for the 19-year-old from Brandywine, Md., was set at $250,000.
Brandon Vandenburg, 20, of Indio, Calif., and Cory Batey, 19, of Nashville, Tenn., are being held on $350,000 bond. Jaborian "Tip" McKenzie, 19, of Woodville, Miss., was booked and released Saturday on $50,000 bond.
Authorities announced the indictments Friday but said the investigation is continuing into an incident June 23 at the Gillette House dormitory.
Vanderbilt dismissed the players from the team June 29.


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Howlin' Wolf Museum
Explore a Blues museum featuring the history and artifacts of Howlin' Wolf and other bluesmen such as Big Joe Williams and Bukka White. The museum is located in West Point, Howlin' Wolf's hometown.
Native American Heritage Sites:
American-Indian Artifacts Museum
Although open by appointment only, this free museum in Columbus is worth the trip. It holds native artifacts dating back thousands of years.
Mississippi Band of Choctaw Indians Reservation
Headquarters of Choctaw Tribal Council, also located on the reservation are the Choctaw Indian Museum, crafts store, and the Pearl River Resort, consisting of two casinos, two championship golf courses and a water park.
Emerald Mound
The second largest Indian ceremonial mound in the nation, built around 1400 A.D. by ancestors of the Natchez Indians, covers nearly eight acres near Natchez, Miss. A trail leads to the top where visitors can view a primary and secondary mound.
Grand Village of the Natchez Indians
This National Historic Landmark in Natchez was the location of the ceremonial mound center for the Natchez tribe from 1200 until 1730 and today includes a museum, educational programs, reconstructed mounds and a dwelling. Downtown Natchez is the oldest permanent settlement on the Mississippi River.
Civil War Sites:
Lee Home Museum
Built by Major Thomas Blewett in the late 1840s, this Columbus home was the former residence of Confederate Gen Stephen D. Lee and now houses Civil War artifacts.
Civil War Interpretive Center (Corinth)
This impressive interpretation center explains military and civilian experiences during the Civil War. Also includes exhibits relevant to African-American heritage. Corinth
Overlooking the Mississippi River, this Federal style mansion in Natchez was named for the French fort built nearby in 1716. Rosalie served as Union headquarters during the Civil War occupation.


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Horde Playing with Fire
Speak to a Master Flame Eater in any Horde capital.
Are you interested in learning some hot new skills?
I don't know if you'll ever be able to match my own considerable skill, but you can probably pick up a trick or two if you're an attentive student. Speak to a Master Flame Eater at any Horde capital. They should be able to help you.
You will also receive:
- 1 83 50 (if completed at level 110)


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Lost Odyssey Cheats :
This cheat for Lost Odyssey [XBOX 360] has been posted at 19 Feb 2008 by cGub and is called "Cheats - Rewards for trading seeds to the Pipot.". If cheat is usable don't forgot thumbs up cGub and share this with your freinds. And most important we have 5 other cheats for Lost Odyssey, look them as soon as possible!
Cheats - Rewards for trading seeds to the Pipot.
by cGub Feb 19, 2008
During your adventures through Lost Odyssey, you may have come across items called "Seeds." You can trade these with Pipots, which are found in pots throughout the game world. Here are the rewards for the number of seeds you give them.
Sorry, to fulfil this action you have to be CheatsGuru User
Lost Odyssey Cheats: |
Sorry, to fulfil this action you have to be CheatsGuru User
• Moshi Monsters cheats, Android • Plants vs. Zombies cheats, NDS, XBOX 360, PS3, IPHONE, Android • Pokemon Heart Gold Version cheats • Pokemon Soul Silver cheats • The Lord of the Rings: War in the North cheats, XBOX 360, PS3 • Shogun 2: Total War cheats • BioShock Infinite cheats, XBOX 360, PS3 • Darksiders 2 cheats, XBOX 360, PS3 • Call of Duty: Modern Warfare 3 cheats, PC, NDS, WII, PS3 • Elder Scrolls V: Skyrim cheats, XBOX 360, PS3 • Torchlight II cheats • Borderlands 2 cheats, XBOX 360, PS3 • Temple Run cheats • Jagged Alliance: Back in Action cheats • XCom: Enemy Unknown cheats, XBOX 360, PS3


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The Horse Forum
- Horse Talk
- - project horse progress!
project horse progress! 4 Attachment(s) Well today I got some pics of him after we were done with lessons. Today we worked on getting him to use and push with his hindquarters. About him: His name is Copper, he was professionally trained but has gotten away with jigging and other small habits and hasnt been ridden in awhile. I dont know his age or breed ( anyone have an idea of what breed he looks like?) Hes also on the thin side because of little exorcize and hes parrot mouthed. Attachment 180089 Attachment 180097 Attachment 180105 Attachment 180113 |
Sorry some of the pics are sideways, I dont know how to fix them. Posted via Mobile Device |
He has such a pretty face! |
Handsome boy! :) Wish you the best of luck with him. |
Thanks :) |
This saturday I will be using him to back up trail rides and maybe take him for a ride around the ranch at the end of the day with the other volunteers, I'll try to take a video and some more pictures. His trot is really smooth too. |
He's a handsome boy!!! Just a little word about that bit - just the tiniest amount of pressure on the rein will cause more pressure in his sensitive mouth than you might know - if you must use a bit at all, (I ride 100% bitless), I would suggest a D-ring snaffle which causes the least pressure. Actually, I shouldn't be shy to say it, but truly, most bits are tools that cause pain. I used to trail ride all the time in our State Parks, and never knew this! Many horse people would protest at such a statement, but it's indeed true through scientific study. I've found it delightful that by using simple rope reins clipped to my horse's halter works wonderfully, and I'm sure they appreciate the chance to prove they can obey commands without the bit. Best of luck with him! :) |
thank you :) Yes, ive heard everything about the tom thumb, and I dont keep contact in his mouth, he neckreins well enough so I dont really have to pull in his mouth. But its also needed because he is barn and buddy sour so I have to sometimes redirect him with the bit. Ill ask about changing it to a Snaffle, but eventually I want to be able to ride him with just a rope around his neck. Posted via Mobile Device |
He looks to have a super kind eye and a great face. I wish you the best of luck with him. Can't wait to see him at a decent weight. |
And I will keep you guys updated weekly. |
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Infectious Diseases |
Virus04/05/2004 |
What is the fifth virus, I have heard many people talking about it, what are the symptoms, and how to fight against it. Thanks
This question has been answered previously. The link below will connect you to that answer. You can also return to the Ask an Expert page and use the "Search" option to find information from related questions. Feel free to write back if you still have questions or need an explanation.
Pamposh Kaul, MD Assistant Professor College of Medicine University of Cincinnati |


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Artsmith is a non-profit organization promoting arts education and the creation of new works.
Saturday, October 8, 2011
Brenda Miller to read November 6
The lyrical and thought-provoking essayist Brenda Miller will read from her breathtaking new collection of essays, Listening Against the Stone, on Sunday, November 6 at 7pm at Kangaroo House Bed & Breakfast on Orcas Island. This latest book explores what Dinty Moore describes as "a captivating account of one woman's evolving and eclectic spiritual path," and Dinah Lenney calls "one of those books you read through in one sitting, then keep close at hand, sustenance for the head and heart." Drop in on November 6 for a little sustenance for your own head and heart, maybe bring a little of your own latest writing to read in the open mic, a forum for local writers to share newly written works before a friendly and receptive crowd.
Wednesday, October 5, 2011
Hello, young golden eagle in the trees in front of our B&B this morning. There are no rabbits or kitty-cats here. Really. You're beautiful, but it's time to move on.
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Can you tell me usually how much genomic DNA should be used in a 50ul PCR reaction using Taq polymerase? The DNA was obtained from a feline cell line.
Submit your paper to J Biol Methods today!
Amount of genomic DNA for PCRamplication
Started by Jason, Jul 24 2001 09:00 PM
1 reply to this topic
Posted 24 July 2001 - 09:00 PM
Posted 26 July 2001 - 09:00 PM
For example,with dna mouse (tail), I soak the tip pipet in dna tube and I soak in pcr mix.I have make pcr with this protocole and it's ok, as with 1 microL,2 microL but no pcr product with 3 microlThe DNA was estimated at 260nm and it was at 400ng/microL so, the amount must be about 500ng/microL.The genomic DNA must be absolutly clean for pcr testgood luke