text:
2017 Croatia Global Money Week
- Croatian Banking Association (CBA)
- Croatian Chamber of Economy (CCE)
- Croatian Employers' Association (CEA)
- Croatian Financial Services Supervisory Agency (CFSSA)
- Croatian National Bank (CNB)
- The Association of managing companies of pension funds and pension Insurance companies (UMFO)
- The Ministry of Finance (Mof)
- The Ministry of Science and Education (MoSE)
- Zagreb Stock Exchange (ZSE)
- Croatian Insurance Bureau (CIB)Education and Teacher Training Agency (ETTA)
- Institute for Financial Education – Štedopis (Štedopis)
- The Ministry of Economy, Entrepreneurship and Crafts (MoEEC)
- 18 896
- 500 000
The celebrations of GMW 2017 in Croatia began with the traditional roundtable discussion of stakeholders, organized by the Ministry of Science and Education and Ministry of Finance, with the latter continuing to lead the GMW campaign in Croatia.
Throughout the Week, the Ministry of Science and Education, along with partners, reached nearly half a million children and youth through an information letter regarding the celebration of the GMW and EMW 2017 that was sent to all primary and secondary schools in Croatia. Alongside actual visits to various financial institutions and informative visits of financial experts to schools, teachers were encouraged to mark the GMW by producing interactive learning materials regarding finance. Also, as a part of the celebration, one school in Zagreb produced several infographics.
The Croatian Chamber of Economy (CCE), in cooperation with the Ministry of Finance, Croatian Financial Services Supervisory Agency, Croatian Insurance Bureau, Croatian Employers' Association, the Association of Managing Companies of Pension Funds and Pension Insurance Companies, Zagreb Stock Exchange and others, conducted a financial literacy awareness session. CCE prepared special promotional and educational materials for all participants. The most interesting is the Pilot Project promotional film "More knowing, better understanding," which includes opinions of pupils about the Project. More than 40 different media channels were included in activities and events.
The Zagreb Stock Exchange (the ZSE) reached around 230 children and youth through visits to the ZSE. Besides educational visits, ZSE also organized a financial quiz for students. The best player won an educational reward - a one day seminar at the ZSE Academy. Young people were also able to engage themselves in the internet educational game called 'Virtual Stock Exchange'.
The Croatian National Bank (CNB) marked Global Money Week 2017 with a series of educational lectures related to consumer protection for almost 700 students from Croatian high schools. The objective of the lectures was to raise students' awareness on the importance of making informed decisions regarding the use of banking services with a sustainable approach to personal finance. The central event of the Global Money Week 2017 at the CNB was a public debate organized together with the Croatian Debate Society on the topic of "End of cash age - advantages vs. disadvantages". The audience, consisting of almost 200 high school students, also participated in the discussion and, at the end, decided the debate winner – pro cash team.
Štedopis, a non-profit organization, and the Croatian Banking Association launched an online quiz (e-learning), "Online Payments," with money for three weeks during the month of March in honor of Global Money Week, and will remain for future free use. Štedopis also hosted local financial education workshops for teens and students, as well as a show on national television to promote the free book "My money, my future," which gives teens and young adults smart lessons about personal finance.
Furthermore, the Croatian Insurance Bureau (CIB) distributed the game "Less Risk-More Fun" to primary and secondary schools in the campaign "Knowledge is the best insurance".
2016 Croatia Global Money Week
- Ministry of Science, Education and Sport
- Ministry of Economy
- Croatian National Bank
- Croatian Financial Services Supervisory Agency
- Croatian Insurance Bureau
- Croatian Banking Association
- Croatian Employers' Association
- Zagreb Stock Exchange
- Croatian Chamber of Economy
- The Association of managing companies of pension funds and pension Insurance companies
- Society 'Our children' Opatija
- 14th - 21th of March
- 8 941
GMW2016 in the Republic of Croatia was coordinated by the Ministry of Finance and involved many other stakeholders from the governmental and financial sectors, including the Ministry of Economy, the Ministry of Science, Education and Sports, the Croatian National Bank and other organizations. Celebrations of the Week started with the traditional roundtable discussion on 14 March, organized by the Ministry of Finance and Ministry of Science, Education and Sport under the title: Financial Literacy and Youth. The main objective of GMW was to encourage the activities and systematic education of citizens, especially youth, in the field of financial literacy. During Global Money Week, a number of activities were organized including round tables, student visits to governmental and financial institutions, student debates, lectures for high school students across Croatia, prize contests, presentations and more. In addition, financial institutions assisted with informing the young citizens about the work of banks, insurers, pension funds, the Zagreb Stock Exchange and other financial institutions.
The Ministry of Science, Education and Sports sent a letter to the principals of schools regarding the celebration of the GMW and encouraged student visits to financial institutions, which were organized in order to inform students about their functions. The Croatian Chamber of Economy organized a conference "Financial Literacy and higher education institutions" in cooperation with the Faculty of Economy in Osijek and the Legal – Economic Clinic in Osijek, as well as other lectures and roundtables during the Week, along with the Zagreb Stock Exchange (ZSE) who hosted educational student visits throughout the Week. Moreover, the Croatian National Bank conducted public debates, panel discussions and a symposium "FORCA 2016: Financial Education – Prudential Life Cycle Activity" on 17 March, as well as special lectures for more than 700 higher grade students of the secondary school from around the country.
The Croatian Banking Association published a Financial Guide for Youth and Infographics on Financial Literacy Measurement results conducted by regulators. Banks hosted students, mostly high school or university students, by holding presentations and lectures for visiting classes, while some bankers visited the schools directly.
Many other events were organized by the Croatian Insurance Bureau, the Croatian Financial Services Supervisory Agency (HANFA), the Association of Managing Companies of Pension Funds and Pension Insurance Companies (UMFO) and other organizations.
Society "Our children" Opatija, together with Children City Council Opatija and Children's Forums of Society "Our Children" Opatija, conducted workshops with children in primary schools on the subjects: "Money and Local Community", "Money, Saving and Budget", "My Piggy Bank for Future" and "Learning About Money and Saving". The objective of the workshops was to raise awareness about the importance of saving by educating children about the history of money, local community and household money flow, differences between needs and wants and the importance of thinking about the future and saving resources. The events aimed to raise awareness about the importance of saving different resources and taking care of the planet and our future, emphasizing that every little act can make big difference. Children from Children's Forums and Children's City Council Opatija were also encouraged to take part in GWM Selfie Challenge.
"Save natural resources. If we use them all, we can't buy them." Student, Croatia
"Don't pollute air, use clean resources, we cannot buy fresh air!"Student, Croatia.
2015 Croatia Global Money Week
- Ministry of Finance
- Ministry of Science, Education and Sports
- Ministry of Economy
- Croatian National Bank
- Croatian Financial Services Supervisory Agency
- Croatian Insurance Bureau
- Croatian Banking Association
- Croatian Employers' Association
- Zagreb Stock Exchange
- Erste Group Bank AG & Croatia
- Society 'Our children' Opatija
- 9th - 17th of March
- 10 402
Croatia celebrated Global Money Week for the second time with numerous activities aimed at raising the levels of financial literacy in the country. On 9 March, the celebrations started with a roundtable discussion on 'Financial literacy – path to contented citizens' organized by the Ministry of Finance and the Ministry of Science, Education and Sport of Croatia. At the round table, the Ministry of Finance presented the National Strategic Framework for Consumer Financial literacy for the period 2015 - 2020 and the Action Plan for the Improvement of Consumer Financial Literacy in 2015. These documents represent the basis of financial literacy in Croatia.
Several ministries and associations organized activities during the Global Money Week with the aim to raise the awareness about managing personal finance and finance knowledge amongst youth and children. Ministry of Science, Education and Sports encouraged primary and secondary schools all over the country to get involved in the celebrations and participate in visits to commercial banks' branches where children could learn about banking system and their work. Based on the recommendations of the Adoption of the Program of Interdisciplinary Curricular Content of the Civic Education in Primary and Secondary Schools, 31 Croatian schools introduced Civil Education, an experimental optional program, for the students in 8th grade. In cooperation with European Commission, Ministry of Economy conducted activities under the campaign "Consumers learn about your rights".
Representatives of Croatian Financial Services Supervisory Agency participated in two round tables discussions on financial literacy and organized a debate on "Pension savings - optimal investment strategy". Croatian National Bank conducted special lectures for more than 300 higher grade students of secondary schools on the topic "Your money - your safety", a financial education activity in the function of consumer protection. The National Bank also prepared two information leaflets for students who participated in lectures during the week. The leaflet on savings "Your money - your safety" provides students with information on the current regulations for deposit operations, while the other leaflet provides information on the capabilities of the central bank in the framework of complying with consumer complaints, titled "What to do if you have a complaint against the conduct of the credit institution?"
The Croatian Insurance Bureau and insurance companies took an active involvement in the celebrations by conducting lectures for students in different regions of Croatia. Croatian Employers' Association organized workshop for its members on the subject of availability of financial services for entrepreneurs and the importance of knowing the financial market offerings. Zagreb Stock Exchange also organized visits for the students from several high schools.
The Croatian Banking Association focused their activities on educating young citizens about the basics of finance. They also prepared educational materials on personal finance and economy. Member-banks organized activities such as lectures, roundtables, visits to the banks, museum and exhibitions about money, involving more than 6000 participants.
Erste Group Bank AG & Croatia organized an interactive exhibition "The ABC of Money" at the Technical Museum in Zagreb. The exhibition primarily aimed at children provided an opportunity to understand the financial world in an innovative and educational manner. The children learned to pan gold, trade in the stock market, open a savings account, repay a loan by cycling, print a bank note, how to save and give donation and much more. From 10-13 March, Erste Group Bank AG & Croatia organized visits for the school children to the exhibition. The exhibition was created by ZOOM Children's Museum Vienna in cooperation with FRida & freD Children's Museum Graz and was initiated by Erste Group as part of the Sponsoring VALUE Program.
Society "Our children" Opatija and Children City Council Opatija also organized a variety of events during the Week, including holding workshops and information stands in Grammar Schools in the region, and organizing a visit of the Governor of the Erste & Steiermärkische Bank Opatija to the Children City Council Opatija on 13 March.
2014 Croatia Global Money Week
- Ministry of Science, Education and Sports
- Ministry of Finance
- Ministry of Economy
- Croatian National Bank
- Croatian Financial Services Supervisory Agency
- Croatian Insurance Bureau
- Croatian Banking Association
- Croatian Employers' Association
- Zagreb Stock Exchange
- 10th - 17th of March
- 300
Croatia celebrated Global Money Week for the first time this year with an inauguration round table discussion called "Reaching Financial Literacy through Financial Education" (Croatian "Financijskim obrazovanjem do financijske pismenosti") held at the Ministry of Science, Education and Sports' premises, joined by the representatives of the Ministry of Finance, Ministry of Economy, Croatian National Bank, Croatian Financial Services Supervisory Agency, Croatian Insurance Bureau, Croatian Banking Association and Croatian Employers' Association. The importance of child and youth financial education was the main topic of discussion. Another round table was held in by the Croatian Banking Association and magazine Banka on "Banks in Economy: Who will Take the Risk".
The Croatian Banking Association held a panel discussion called "Money, Banks, Financialization" ("Novac, banke, financijalizacija") at a public library in Zagreb. Prior to the event, the Ministry of Science, Education and Sports sent a letter to all high schools informing them of the celebration and the possibilities to learn more about the Week and financial issues.
A "Croatian Employers' Association Working Lunch" was held in Zagreb where a discussion on monetary and fiscal issues concerning the youth. At the University of Rijeka, in the Faculty of Law, the Croatian Insurance Bureau held a lecture for students on financial literacy, while at the University of Zagreb, Faculty of Economics, the Croatian Insurance Bureau held a lecture for students on financial literacy.
During the week, high school students visited the Croatian National Bank, Zagreb Stock Exchange, as well as some commercial banks and insurers where they learned about the different aspects of their financial responsibilities and options.
Publications, designed for different age groups, were produced and distributed by the Croatian Banking Association and the Croatian Insurance Bureau who distributed the "Mini Banking Dictionary" for children and "What is the Actual Purpose of Insurance, respectively.


text:
Universal Studios Japan is once again teaming with Capcom (previously the two did a live-sized Monster Hunter attraction) for "Halloween Horror Night". Until November 11, the theme park will turn into Resident Evil at night. So, theTerminator 2 Cyberdyne Systems building becomes the Umbrella Corporation and a walk through attraction, while the New York City area doubles as Raccoon City, thanks to some fancy projection mapping. The recently opened Biohazard Cafe and Grill in Tokyo also used 3D projection mapping effects to bring creatures to life.
Full Story: Kotaku

subset: commoncrawl


text:
Bid Information
Bid Alert No: 00000460949
Bid Title: Bituminous Cold Patch
Agency Bid No. Title: 21016-26
Received Date: 01/15/2016
Close Date: 02/04/2016
Purchase Type: Not Stated
Delivery Point:
Delivery Date: Not Stated
No additional specifications were given.
Bid Related DocumentsDownload Solicitation Notice
Product Code: 74514
Agency Information
Issuing Agency: Isabella County Road Commission
State: Michigan
Agency Type: State and Local
Contact: 2261 E. Remus Rd, Mt. Pleasant, Michigan, 48858


text:
Radio | Sep. 24, 2016
Sept. 24, 2016 Joan Desmond on Economic Benefits of Religion/ Peter J. Smith on Georgetown Slaves
This weekend on Register Radio, Matthew Bunson, senior editor with the National Catholic Register, be joined by senior editor Joan Desmond to talk about the economic benefits of religion. And then I will discuss the history of the Georgetown slaves with Register reporter Peter Jesserer Smith. That's Register Radio, every Saturday at 7pm Eastern, and Sunday at 11am Eastern, on EWTN Radio.
To download the audio, right-click on the play button and select 'Save/Download Audio As...'
Join the Discussion
We encourage a lively and honest discussion of our content. We ask that charity guide your words. By submitting this form, you are agreeing to our discussion guidelines. Comments are published at our discretion. We won't publish comments that lack charity, are off topic, or are more than 400 words. Thank you for keeping this forum thoughtful and respectful.The time period for commenting on this article has expired.
Comments are no longer being accepted on this article.
EWTN NewsLink


text:
That the massive feeding programs which are going in this country for quite some time is a "golden goose" for at least some people like the private manufacturers and the controlling "babudom" , is well known. Why the governments at the center and states close their eyes to such blatant corruption is also known, the main reason being the unholy nexus between the politicians in power aided by the babus and the manufacturers with no conscience regarding the harm they are doing through their devious practices. Under the guise of economic liberalization, governments in almost all states have roped in private manufacturers for supply of nutritious food to beneficiaries under various state sponsored feeding programs, most of them being children. On the other hand they have systematically sidelined their own specially set up organizations, in many cases forcing them to wind up in absence of adequate funds. The consequences are there for every body to see! If an estimate is to be made regarding the looting of public funds through such apparently "noble' schemes, it could run into thousands of crore of rupees! A recent report from Orissa typically exemplify this national malaise.
"The Orissa Vigilance Directorate has submitted a status report in the High Court on its probe into the multi-crore pulses scam, saying pecuniary advantages to the tune of nearly Rs 20 crore were made by government officials by showing undue favours to traders. According to the Directorate, pecuniary advantages to the tune of Rs 19.62 crore were made by certain government officials by showing undue favour to private suppliers and traders in the districts of Balasore, Jajpur, Ganjam, Mayurbhanj and Deogarh. As a result, poor and substandard quality of pulses were supplied during implementation of feeding schemes in 2009-10 and 2010-11, said the report submitted yesterday. School students, children, pregnant and nursing mothers coming under midday meal (MDM), supplementary nutrition programme (SNP) and emergency food programme (EFP) in these districts were provided with mould-grown and substandard pulses, it said. The report further said that at least one criminal case each was registered in all these districts in which a total of 34 accused persons were arrested ever since the vigilance registered cases over the scam earlier this year. "All the arrested persons were, however, released on bail as per orders of the High Court, the report said. The investigating officers have also examined at least 87 witnesses and seized a total of 152 documents from all the five districts, the report said, adding further investigation in the cases are on. Detailing the findings from each of these districts, the report said samples collected from different feeding centres were sent for laboratory tests to Bhubaneswar-based State Drugs Management Unit. "In most of the samples, the analytical reports said the pulses did not confirm to the prescribed standard and were highly infected and of poor quality due to presence of insects in it," the report said. The irregularities in supply of pulses was brought to the notice of vigilance sleuths by Bari MLA Debashis Naik last year. The then Women and Child Development minister Pramila Mallick resigned over the scam, the state government took action against three district (Deogarh, Mayurbhanj and Balasore) collectors".
Is it not a sorry state of affairs in this country that despite tons of evidence regarding the corruption being indulged in by "the high and the mighty", these criminals roam around the country in style with least impunity, regret and remorse? They can be rightly called the "Merchants of Death", as these unsafe foods can cause health damage and eventual mortality in the long run. Will the curse and desperation of millions of kids, who are deprived of their legitimate share of food provided from public funds, ever spare these culprits in the long run? May be the citizens in this country are no where to go except the God!


text:
The name's Dessie and I am twenty years old, currently in my first year of university, studying English Language and Literature. However, I am looking into transferring to a Creative Writing degree in the upcoming academic year.
Books have surrounded me my whole entire life, even before I learned how to read them. My grandfather was a huge book worm and I like to think I inherited that trait from him. I would say I've always been an avid reader, however, it wasn't until my first year in secondary school that I rediscovered my love for reading. That was when I fell in love with YA literature and have not let go since. That said, my reading preferences are not limited to just YA as I often times find myself engrossed by New Adult and Adult novels just as much. My favourite genre has to be Fantasy, though Science-Fiction comes a close second.
As well as a reader, I am also a writer. For me, it is impossible to be one without the other. One cannot write well if one does not read. I have been writing almost as long as I have been reading, starting with little bits of poetry and coming up to short stories and even working on novels (though I can never quite finish them.) Don't be surprised if you ever see a post of me ranting about writing.
I started this blog as a way to gather my thoughts on the books I read and also be a more active part of the bookish community. I'm usually terrible with commitment, but I hope my constant reading will prompt my updating of this blog, even if it does take a while to actually update.
Feel free to message me in any of my social media if you want to chat!


text:
The Boston Public Library is pleased to host MARJORIE GARBER for a lecture entitled, "Desperately Seeking Shakespeare." GARBER has published seventeen books and edited seven collections of essays on topics found in Shakespeare's work and was a featured commentator on the BBC/PBS television series, "Shakespeare Uncovered." Newsweek magazine chose GARBER's Shakespeare After All as one of the five best nonfiction books of 2004.
With the recent attention to Shakespeare's life and work through commemorative celebrations (the 450th anniversary of his birth in 2014; the 400th anniversary of his death in 2016), there has been a renewed quest to find something about Shakespeare that would explain his astonishing accomplishment. GARBER's talk will review some of these investigations, and the major questions about Shakespeare that seem to underlie them.
This event is free and open to the public.
Certain books were "banned in Boston" at least as far back as 1651, when one William Pynchon wrote a book criticizing Puritanism.


text:
The rantings of a wife, mother, amateur baker and RCP with poor sleep hygiene.
Friday, January 28, 2005
Infant Geographia
Here we have the human baby, Egyptian-Portugese variety, out of his natural habitat, at a a celebration of Xmas. The infant is out of his comfort zone--his element, and does not yet understand the gathering. He appears confused and scared, as he reaches for the comfort of something he recognizes from home-- the television remote control.
This is That Baby at his Great Uncle Gary and Aunt Gails house for Xmas this year. He was in rare form people, lemme tell ya.
Subscribe to: Post Comments (Atom)


text:
Once in a while, we break the monotony with a rare and vibrant "multilingual" product: JESSICA SANCHEZ, a Filipino-Mexican-American who was born on August 4, 1995, in San Diego, CA. Jessica's maternal grandmother is from Manila and her maternal grandfather is from Bataan. Her mom, Editha, also hails from Bataan. Her dad, Gilbert, a Mexican-American, originally hailed from Texas.
On the March 7, 2012, episode of "American Idol", 16-year-old Jessica belted what is widely regarded as one of the most difficult pieces in the whole world — I Will Always Love You — one of the late Whitney Houston's signature pieces and, well, watch (and I hope you vote for her):


text:
To examine the relationships among negative attributions of friend and peer reactions to diabetes management in social situations, anticipated adherence difficulties, friend support, diabetes stress, and metabolic control.
Results Structural equation modeling demonstrated an excellent fit of two models depicting the mediating role of anticipated adherence difficulties and diabetes stress on the relationship between negative attributions of friend (first model) and peer (second model) reactions and metabolic control. Friend support was found to moderate the path between diabetes stress and metabolic control in an unexpected manner. That is, as friend support increased, so did the relationship between stress and metabolic control.
Conclusions Adolescents who make negative attributions about reactions of friends and/or non friend peers are likely to find adherence difficult in social situations and have increased stress, with the latter associated with metabolic control. Results are discussed in terms of a social information processing model of adjustment.
Management of Type I insulin-dependent diabetes involves adherence to a series of complex daily behaviors such as monitoring blood glucose levels, injecting insulin, carefully maintaining diet plans, and exercising. The maintenance of these adherence behaviors is seen as the best way to maximize a long-term positive prognosis and decrease the likelihood of short-term complications (e.g., hypoglycemia, hyperglycemia, ketoacidosis) and long-term negative consequences (e.g., renal failure, retinopathy).
Even though adolescents possess greater diabetes knowledge than children, they tend to have more problems with adherence. Social situations, especially those involving friends, may negatively impact the adherence behavior of adolescents and adolescents report that adherence difficulties are more frequent in social and peer contexts (Berlin et al., 2006). Some adolescents may feel unable to maintain their regimen within these contexts because they anticipate peer pressure or are apprehensive about being singled out by others . These results suggest that cognitive appraisals of social situations may be related to adherence behavior.
Decisions to avoid adherence behaviors around friends are problematic because adolescents are missing opportunities for support. Despite adolescents' apprehensions to the contrary, friends of teenagers with Type 1 diabetes have been found to provide both emotional and companionship support and support for some aspects of the treatment regimen.
One possible reason for this lack of relationship is the role of attributions and cognitive appraisals, which when positive may serve as a protective factor and when negative may impact the adjustment of adolescents with chronic illness (Wallander & Varni, 1992).
The role of friend support was only found to moderate the path between diabetes-stress and metabolic control. Thus, it appears that friend support may not have a large impact on the nature of the relationship between attributions of others' reactions and anticipated adherence difficulties, or the relationship between adherence difficulties around others and diabetes stress. In a seemingly paradoxical fashion, as friend support increased, so did the relationship between stress and metabolic control. A few interpretations of this finding are possible. First, for adolescents experiencing greater stress or poorer metabolic control, their friends become more supportive, but these friends may not be effective in helping them manage their diabetes-related difficulties. Alternatively, adolescents with higher diabetes stress may not make good use of coping support from friends, or they may actually find increased friend support as aversive. Finally, the support efforts by friends may actually be maladaptive, encouraging the adolescents to make poor behavioral choices. These alternative explanations regarding the impact of friends should be examined in future research.


text:
I had a great day today spent with my cousins two kids who are 6 months and 2 years old, in London. We ended up going to the Tate Modern briefly and this is part of an installation by whom, unfortunately I do not know. The part I like most was the silvery thread it is suspended from.
07/04/09 just found out thank to my mum what this was, click here


text:
Flaming Lips
In the current American music lexicon, The Flaming Lips have become synonymous with festivals.
Charting the course to this synergy-soaked point begins decades after the trio formed in Oklahoma City and developed a cult following, mostly with the indie/art crowd, for brilliant albums and raucous live shows. Perhaps this path to their current status began in the pre-dawn hours at Bonaroo in 2002, when the Lips took a wrecking ball to the jam-band paradigm when the band's sensory-overload show -- complete with confetti, video, dancers in animal suits and copious amounts of fake blood -- produced a peak-fest for all the night trippers and forced an entire scene to take notice. Another mile-marker came during 2004's Coachella, when frontman Wayne Coyne moved atop the crowd in a giant plastic bubble, providing the most publicized photo for an event many music pundits consider the biggest and best among the current roster of annual festivals. Halloween weekend last year, the Lips mercifully revived the currently impotent concept of music-as-statement at the Vegoose festival, where they closed their set by covering Black Sabbath's "War Pigs," the tune's theme illustrated by a kaleidoscopic montage that shone light on murky issues of violence and greed.
"We've always tried to play shows that are just barely contained, to put on a big spectacle that gets everyone involved," Lips bassist Michael Ivins says during a recent phone interview from his home in northern Kentucky. "The festival format is conducive to that. It's a different mood, as people are hanging out together all day and all night, often camping. There's more freedom, a chance to get out of your head a little bit and just let go."
The popularity of The Flaming Lips at festivals is indicative of the increased popularity of festivals themselves in recent years.
"I think we're catching up with the festival culture in Europe, where it's been a big part of the musical culture for 30 or 40 years now," Ivins says. "You pay for one ticket and get to see a bunch of different bands. It really took off here with what used to be the jam-bands. But that scene was really just a crowd of people that are really just fans of music. That embrace all kinds of music. That don't really identify themselves with one style. That won't respond to pressure to, 'Dress this way. Act this way. Listen to this type of music.'"
The Lips' style is difficult to identify as well. Their studio work, dating back to their self-released, self-titled debut in 1984, has always shared traits of melodic ingenuity and ambient, abstract songwriting applied to an adventurous production philosophy. Ivins considers long-time producer Dave Fridmann "a hand-in-hand, integral part of the process," the studio an instrument unto itself and the band a production team, one in which members often switch instrumentation and roles. Writing credits are shared, and individuals (Steven Drozd, Ivins and Coyne) are not identified by what they play.
Their studio work has become increasingly complex and innovative, especially beginning with 1997's Zaireeka (Warner Brothers). This summer, they released the superb At War With The Mystics, an album certainly informed by the current ennui, but a work that's much deeper than a simple rant against the Bush's administration, the Iraq war and the religious right. It's more universal than that. The Lips paint their own picture of the world, applying coats of Day Glo over the drab and mundane like maniacal Merry Pranksters.
Sure, At War With The Mystics is subversive (as "The W.A.N.D. (The Will Always Negates Defeat)" points out, "We got the power now motherf--ers"). Sure, it's political. But there are many other levels to be explored as well.
"Some songs, like 'Haven't Got a Clue,' to which people are hanging various labels and interpretations, aren't really about Bush or politics per se," Ivins says. "We're looking at the individual condition, and expressing the human condition. What does it mean to be a person going about your life, living with other people and animals and the environment around you. How do you deal with all that stuff?"
Here are a couple of ways: Create a cosmic, supernatural sound. Or for us mortals, go to a festival, immerse yourself into the Voodoo experience and see the Flaming Lips. -- Frank Etheridge
5:30 p.m. - 6:30 p.m. Sun., Oct. 29
Voodoo Stage
Drive-By Truckers
Back for the first time in more than a year, the Drive-By Truckers roll into town for a gig at the Voodoo Festival and a benefit Saturday night at Tipitina's. It's been a good year for the Truckers. They did a great opening stint for Son Volt and one for the Black Crowes. The band won duo/group of the year from the Americana Awards in Nashville. And they put out a new record, A Blessing and A Curse, which shows their sound evolving without sacrificing the power of their rock 'n' roll or great songwriting.
The Drive-By Truckers started in the mid-'90s as a hard-driving rock 'n' roll band. Guitarists and songwriters Patterson Hood and Mike Cooley had kicked around in bands for years before getting the Truckers together in Athens, Ga. Their first records had crunching guitars and songs about people and characters they knew. Their approach was reminiscent of Molly Hatchet and Lynyrd Skynyrd, and this came to the forefront in their double album Southern Rock Opera. There were songs about the rise and fall of Lynyrd Skynyrd as well as lyrics dealing with what it meant to grow up in the South in the '70s. Their music had great nuance making deeper statements still rocking so hard they could peel paint. The Southern Rock Opera earned them well-deserved press and their next two CDs were filled with the same themes of growing up Southern, dealing with the multiple legacies of such complexities, and the choices one is forced to make in those situations. The latest CD, A Blessing and A Curse, continues these themes while mostly toning down the three guitars played by Hood, Cooley and Jason Isbel, but not entirely, as evidenced in the binge-gone-wrong song "Aftermath, USA."
The last time the Truckers played in town was Friday, Aug. 26, 2005. It was their usual loud, in your face, kick-ass rock show. Little did anyone know what the future might hold. For many, it was the last show they saw in New Orleans before Katrina hit, the deluge flowed, and our lives changed forever. Seeing the Drive-By Truckers is always a cathartic experience, but these sets will be special -- another lens by which people can look back at how far we've come and look forward to places we need to go. But besides that, right now there may not be a better rock 'n' roll band on the planet. -- David Kunian
5:30 p.m. - 6:30 p.m. Sat., Oct. 28
PlayStation/Billboard.com Stage
Shooter Jennings
From the back of his tour bus in Columbus, Ohio, Shooter Jennings sounds like a cheerful man. "I'm just hangin' out on the bus, with my brand new dog. No, that's my girlfriend. She's just acting like one. I wish we had a dog, though. How are you doin'?" He's in a pretty great mood for someone whose latest album, Electric Rodeo (Universal South, 2006) is composed of hard-life tunes like the title track that tell the familiar story of the road-weary rocker ("All I know is a guitar and a bottle").
"Well, when I was a kid, I didn't understand why 'Tonight The Bottle Let Me Down' was such a good song, because I didn't live it," he says. "There is something to that, having to have sorrow under your belt. And there's a lot of influence here from other artists. A lot of my influence on that record was Hank Jr. But it's all true -- even if we haven't got there yet, it's right around the corner for us."
Whether or not Jennings is looking forward to paying that hefty price of fame, he deserves it -- the fame part, anyway. Electric Rodeo contains more good ol' boy badassery than a pickup truck full of Pabst Blue Ribbon. You can practically hear the smoke hissing off the fiddle strings on the manic "Manifesto No. 2." And blues legend Tony Joe White lends his unmistakable, nightmarish growl to the thumping "Alligator Chomp" -- an apocalyptic, allegorical song about war and hatred between animals in a swamp that ranks with some of White's weirdest, most haunting rock numbers. The band presented the song to White to record his vocal track in the studio on the spot. Jennings says, "Some fourteen-year-old-kid wrote in to ask what it was about, and we told him it was about the assassination of hope. I loved the concept. I love the song. It's so not ready for radio."
It's probably not -- but that's not a bad thing. This is pure country in the best outlaw, boot-stomping vein, and Shooter's dad, of course, headed up the boy's club that wrote the book on that. In recent years, factions in Nashville have tried to bring rowdy back to the table and cut through the poppy treacle of acts like Faith Hill with dive-bar hellraisers of a sort, like Gretchen Wilson, but you can't mass-produce the kind of down-home rock Jennings obviously has coursing through his veins. It doesn't hurt that he's the progeny of Waylon and Jessi Colter -- whose name he has tattooed on his forearm. But Shooter's guitar-driven Southern rock has a wry wit running through it that keeps it honest, and that's where he shines like the reflection of neon in a puddle of spilled beer. It's not enough to just write a song that has a dog, rain, prison, a truck and your mama in it to get a real country song; these days, it's having a sense of humor about the whole country template that makes it all fall into place. Love Jennings for songs like the title track, which echoes his dad's "Are You Sure Hank Done It This Way" uncannily, or love him for the hilarious "Aviators," which has a stoic Southern man apologizing for a long list of misdeeds, including hitting on his girlfriend's mom and shooting her dog ("If our love won't work now, darling, maybe later / you can't see my tears behind my aviators"), but if you are a red-blooded American with rock 'n' roll in your heart, it's for sure that love him you will. -- Alison Fensterstock
4:30 p.m. - 5:30 p.m. Sat., Oct. 28
Voodoo Stage
- Michelle Martin-Coyne
- Flaming Lips


text:
The problem isn't really a new
one. It's been growing for some
time. Literally.
I wrote about our early morning
blackout that left my place and some
300 other homes without power this
past Wednesday night. Well it seems
the problem was a bush! No. Not
either of those President guys. I mean
"bush" as in bushes, limbs, and branches.
Shrubbery and trees that have grown
so high they've flirted with power
lines running through our area. And
when they get too close to each other
sparks fly! And lights go out!
Yesterday a fleet of orange
colored trucks rolled in to our
development. You've probably seen
them before. Each bears some
Swedish looking name that starts
with an A. I never have been able
to pronounce it.
The crew members started
hacking away at any growth that
came anywhere near any of the
electrical wires. I'm sure the birds
and a few of the squirrels will be
unhappy. But if all that cutting
keeps the current running I'm all
for it!
Hope it works and that all
your NEWS is good!


text:
Yes, Caroline is wearing a pull-up on the outside of her pants. I do realize they work better if they are under the clothes. She saw a commercial for them today and ran in the bathroom to put one on. I have been unable to get her to take it off!
Monday, May 5, 2008
I know this is not a great video clip. I am playing around with the video feature on the blog and my camera.
Posted by Emily and Andy at 4:54 PM
3 comments:
- darlene said...
LOVE IT. I could see and hear the jumping as well as the talk.
Caroline is so funny. Everyone who has ever had children understands her deciding to put on something and refusing to take it off. Just created embarassing shots for you to share when she is older.
- May 5, 2008 at 5:30 PM
- sunni said...
Great Job on the video! I love the pull-ups that is so funny:)
On the picture issue, you can use a photo editing software to crop the picture before adding it to your page. GIMP is a free download from CNET which is what I use.
- May 5, 2008 at 6:12 PM
- robynsbowtique said...
Love the pullup. Agree with Darlene.
- May 7, 2008 at 11:38 AM
Subscribe to: Post Comments (Atom)


text:
All Categories
Enjoy the comfort and convenience of desktop typing on your smartphone, and tablet with this mobile keyboard.
K380 Multi-Device is a wireless keyboard that connects to allBluetooth® wireless devices that support external keyboards.
- Bluetooth wireless
- Multi-platform/cross-platform
- Windows®, Mac, Chrome OS™, Android™, iOS
Supreme Computers India Pvt Ltd. is a proven market leader in the supply of excellent IT Products in India. Ever since its inception in the year 1994, we have steadily risen up the ladder in this industry and are today one of the Leading IT Resellers in South India. Today we are the authorized partner for world's best IT Brands, which includes HP, Apple, Samsung, LG, Linksys, Microsoft, Seagate, Intel, and VIVO Etc. The success of a business is not only about achieving it, but also more about maintaining. We have a team that keeps evolving with the demands of the market, thereby keeping with the flow. Goodwill and reliability also play an important part. We have been associated with the world famous brands for so long that it reflects in the way we do business.


text:
Family Law
Ask a Family Law Question, Get an Answer ASAP!
Hi Claire,
To be honest, I am not sure what kind of external support my grandmother is getting at the moment as I have not seen her for almost 8 months. At the time of the falling out between my mother and grandfather, my grandma began getting support from 2 community nurses who would help her with personal care. I am not sure if social services are involved. My mother is her only daughter and she has not been able to see her for almost 8 months.
Speaking to my uncles is not really an option as our relationship has completely deteriorated. My mother is not really fussed about her relationship with her brothers, she just wants to be able to meet her mother even if its only once a week or even once a month.
I am not sure but if someone did hold Power of Attorney for my grandmother, it would be one of my uncles.
I have been in touch with the Office of the Public Guardian and they do not have any record of a Power of Attorney for my grandma. How will social services be able to intervene? Should I get my solicitor to write to social services?


text:
Former allies go at each other's throats for water in Brian K. Vaughan and Steve Skroce's We Stand On Guard TP (9781534301412, $14.99) from Image Comics. Read this exclusive interview with the book's creators!
Vince Brusio: Did We Stand On Guard start out as a Brian K. Vaughan project, and then it was enter-stage-left Steve Skroce? Or did the two of you collaborate on this from the beginning? Tell us how the seed got planted.
Brian K. Vaughan: No, there's definitely no We Stand On Guard without Steve Skroce. He and I met a while back at a movie screening, and while we're both huge fans of Hollywood and are enormously grateful to have gotten to do some work out there (and Steve's contributions as a storyboard artists are much larger and more important than mine as a screenwriter), I like to think that we're both comic-book creators at heart, and that we were both looking to get back to this glorious medium in a more full-time way.
I'd originally come to Steve with a germ of an idea for a more supernatural project, one we still hope to return to someday, but it was listening to Steve talk with my wife (who also happens to be a native of Canada) that first planted the seed for this story, which I hope combines some of the harder elements of science fiction with the spectacular visuals and social relevance that Steve and I both dig in our fiction. I've been lucky enough to work with some amazing artists in my career (most of whom happen to be Canadian!), but I'd never be able to tell this one with anyone but Steve Skroce.
Vince Brusio: So the threat to America isn't the government purchasing all of the AR-15 ammunition, it's people from Canada that want their country back? Why Canada? They have such great beer! Does Canada has enough firepower in the future to go toe-to-toe with the U.S.? Was there an arms race?
Brian K. Vaughan: Well, Canada is to water what Saudi Arabia is to oil, and while there's an inciting incident in our first issue that leads the U.S.A. to invade and eventually occupy Canada in what the Americans claim is a war of necessity, the Canadians believe that we're really just coming to take their H2O, especially because our future United States is suffering from the kind of "megadrought" many scientists believe is a real possibility.
But yeah, Canada is definitely outgunned in this conflict. In the 22nd century, the U.S.A. is still the world's foremost military superpower, only now, we also have giant f***ing robots. So our story will involve a lot of asymmetrical battles with technologically mismatched opponents, but history has shown that those can still make for very long, very bloody wars.
Vince Brusio: We Stand On Guard is described as a "military thriller set 100 years from now." Is this you two guys totally shooting from the hip, or were there previous works that have stayed with you since adolescence that you can cite as inspiration (comics/anime/film)?
Brian K. Vaughan: As always, I'm most influenced by my own life (in this case, friendly arguments with my in-laws during Canadian Thanksgiving, and with my collaborators at conventions), but I'd be lying if I said movies from my childhood as diverse as the original Red Dawn and the first animated Transformers weren't also part of this book's DNA. I also took a lot of inspiration from Das Boot, and an incredibly relevant old film called The Battle Of Algiers.
Steve Skroce: Yes to all that! The movies and comics of the 80's are a big influence on me, John Milius's Conan and Red Dawn are high on that list, and Otomo's Akira is a huge influence. It showed science fiction war and destruction on a scale I had never seen.
Vince Brusio: What was the most challenging aspect of creating We Stand On Guard?
Brian K. Vaughan: No challenges for Baldy! I've got the easy job. I get to work with a legendary artist doing some of the most detailed work in the history of monthly comics, an equally talented colorist, Matt Hollingsworth, playing at the proverbial top of his game, and Saga's own deservedly world-famous letterer/designer Fonographiks. All I have to do is stay out of their way.
Steve Skroce: The challenge is trying to live a balanced life when all I want to do is get back to the drawing board! I've loved Brian's work for years and I'm loving this story! Matt Hollingsworth and Fonographiks are my dream team and I'm feeling blessed having them back me up. Brian also never laughs when he hears my say 'out' and 'about'. I appreciate that!
Vince Brusio: If you had to create a grass-roots marketing campaign yourself to get people pumped up over this book, what materials would make their way into the press kit? Spent bullet shells? Recorded audio from the battlefield? What would give people an accurate picture of what they're about to see in We Stand On Guard?
Brian K. Vaughan: I'd include a double-double, a nice two-four, and a voucher for a free month of hydro. And if you don't know what the hell any of that means, get ready, because a bunch of badass Canadian freedom fighters are coming to blow up your robots.
Steve Skroce: I'll definitely take that free month of Hydro. I'd also like to include a delicious poutine, it's an amazing dish of french fries, cheese curds and hot gravy with sprinkled bacon bits on top ( an added element of my design) Poutine is known throughout Canada as the dish most responsible for our socialized health care system.
Thanks, eh?
Vince Brusio writes about comics, and writes comics. He is the long-serving Editor of PREVIEWSworld.com, the creator of PUSSYCATS, and encourages everyone to keep the faith...and keep reading comics.


text:
|Woo Hoo!!!
Nov 19, 2005
I just got tested after having barebacked w. 2 guys..3 months ago....I came out negative..and I'm so happy...Guys get tested..I was afraid, but you know what live up to your S**T and be a man and do it...I feel so alive and will never do the same error again...
Response from Dr. Frascino
Thanks for writing in! Oh, and it's O.K. to use the word SHIT here, even though Bush and his religious zealots would prefer we change "Shit happens" to "Manure occureth" to make it sound more Biblical.
Stay well!
Dr. Bob
Does most health insurance cover HIV treatment?
Confusion about the early HIV test. HELP DOCTOR BOB.
This forum is designed for educational purposes only, and experts are not rendering medical, mental health, legal or other professional advice or services. If you have or suspect you may have a medical, mental health, legal or other problem that requires advice, consult your own caregiver, attorney or other qualified professional.
Experts appearing on this page are independent and are solely responsible for editing and fact-checking their material. Neither TheBody.com nor any advertiser is the publisher or speaker of posted visitors' questions or the experts' material.
Review our complete terms of use and copyright notice.


text:
Attenuated Cardiac Allograft Vasculopathy in Mice With Targeted Deletion of the Transcription Factor STAT4
Jump to
Background—To study transcription factor signaling pathways that mediate cardiac allograft vasculopathy, we used mice with targeted gene deletion of signal transducer and activator of transcription (STAT)4 and STAT6 as recipients in our mouse cardiac transplant model of chronic rejection.
Methods and Results—At day 55 after transplantation, cardiac grafts placed into STAT4 −/− (n=10) had reduced frequency (24±2%) and severity (9±4%) of vascular occlusion compared with wild-type controls (n=7, frequency 70±12% [P<0.001], severity 25±6% [P<0.05]). This decrease was associated with reduced intragraft expression (32P RT-PCR and immunohistochemistry) of the Th1 signature cytokines interferon-γ (P<0.001) and interleukin (IL)-2 (P<0.001). Furthermore, cardiac grafts in STAT4 −/− had fewer infiltrating CD45+ mononuclear cells (99±27 cells/mm3 compared with 551±168 cells/mm3 in wild-type controls [P<0.05]) and reduced expression of P-selectin (P<0.001) and E-selectin (P<0.01) ligand, recently shown to regulate Th1 cell recruitment. In contrast, in grafts placed into STAT6 −/− (n=11), the development of cardiac allograft vasculopathy (frequency 62±8%, severity 28±6%) and Th2 cytokine profiles (IL-4, IL-10) were comparable to those in wild-type controls.
Conclusions—Hence, we show that immune responses mediated by STAT4, but not STAT6, contribute to the development of cardiac allograft vasculopathy. We speculate that when present, STAT4-mediated signaling pathways may promote cardiac allograft vasculopathy by directing Th1-specific lymphocyte recruitment, activation, and effector functions.
After solid organ transplantation, chronic rejection with the development of cardiac allograft vasculopathy is the major impediment to long-term graft survival. Experimental models have helped us to begin to understand the pathogenesis of this form of vascular occlusion. The regulation of the pathogenesis has been hypothesized as a coordinate and interactive event involving various inflammatory cell types. In addition to B lymphocytes and macrophages, T lymphocytes have been identified to play a crucial role.1 2 3 On activation, T lymphocytes differentiate into Th1 and Th2 subsets, characterized by differences in their cytokine repertoire,4 5 adhesion mechanisms,6 7 effector function profile, and character of local immune response.4 5 Transcription factors have been identified that regulate these Th1 and Th2 cell differentiation programs in a proximal manner. The signal transducer and activator of transcription (STAT) protein family includes STAT4, which controls interleukin (IL)-12–mediated Th1 differentiation.8 9 Mice with targeted deletion of STAT4 signaling show impaired differentiation of Th1 cells in response to either IL-12 or Listeria monocytogenes,8 9 whereas targeted deletion of STAT6 interrupts Th2 differentiation.10 11 12 Lymphocytes from STAT6-knockout mice have defective IL-4–mediated Th2 differentiation and proliferation.10 11 12 These mice provide the opportunity to examine the role of transcription factor pathways regulating the Th1 or Th2 pathway.
To study the impact of these transcription factor programs on graft vasculopathy, we used STAT4- and STAT6-knockout mice as recipients in our heterotopic cardiac transplant model of chronic rejection.2 13 14 We assessed the effect of recipient STAT4 or STAT6 deficiency on intragraft cytokine profiles, arteriosclerotic lesion development, Th1/Th2-specific cytoadhesion signaling, and leukocyte recruitment.
As heart donors, inbred male CBA/CaJ (H-2k) mice 6 to 8 weeks old were used. As recipients, male C57BL/6J×129Sv (H-2b) mice with targeted deletion of STAT4 (STAT4 −/−, n=10)9 or STAT6 (STAT6 −/−, n=11)11 were compared with C57BL/6J×129Sv wild-type controls (n=7, hereafter referred to as wild-type controls). The genotype of the knockout mice was confirmed by polymerase chain reaction (PCR) using genomic mouse-tail DNA. The knockout mice were all backcrossed 6 times to C57BL/6J (F6). F2 hybrids were used as physiological wild-type controls. Because these F2 mice provide only an approximate genetic match, we had to confirm that potential differences were not due to a more dominant 129Sv influence in this group. Therefore, inbred wild-type mice on a pure C57BL/6J background (n=8, hereafter referred to as inbred controls) were used as a second comparative control group. STAT4- and STAT6-deficient mice were generously supplied by Dr James N. Ihle (St Jude Children's Research Hospital, Memphis, Tenn). CBA/CaJ (stock No. 000654), C57BL/6J×129 Sv (stock No. 101045), and C57BL/6J mice (stock No. 000664) were obtained from Jackson Laboratory (Bar Harbor, Me).
Heterotopic Mouse Transplant Model of Chronic Rejection
Vascularized, heterotopic abdominal transplantation of the donor heart was performed, monitored, and harvested at day 55 as previously described.2 13 14 15 16 Immunosuppressive therapy to prevent acute parenchymal rejection and produce grafts undergoing chronic rejection consisted of anti-CD4 and anti-CD8 (GK1.5 and 2.43, 2 mg IP on days 1 to 4 and then weekly to day 28).13 14
Cytokine Expression
Relative gene transcript levels were measured by reverse transcription (RT)-PCR from graft cDNA panels as published previously.2 13 14 15 16 In this model, in which the small size of allograft tissue is limiting, 32P RT-PCR offers the advantage of studying a large number of factors in replicate in a semiquantitative manner. PCR primers were designed with MacVector 5.0 (Oxford Molecular Scientific), and specificity of the primer sequence was confirmed by basic local alignment search tool (BLAST) analysis. For each individual primer pair, specific annealing temperature and cycle number were optimized by serial annealing studies, PCR cycle studies, and cDNA dilution studies. The logarithmic ranges of amplification were established as previously described17 to ensure that the amplified PCR product reflects the original levels of transcription. Primer sequences, sequence accession numbers, optimized annealing temperatures, and cycle numbers are listed in the Table⇓.
Triplicate samples were amplified with AmpliTaq Gold DNA polymerase (Perkin-Elmer). [32P]dCTP (150 000 cpm per reaction) was included to quantify the amplified product. Incorporated [32P]dCTP in amplified products from dried agarose gels was measured by volume integration (Molecular Dynamics). Corrected levels of the specific transcript were derived by dividing the amplified product value by the mean value for the control gene G3PDH in the respective sample and reported as mean±SEM for all grafts in each recipient group.
To confirm the presence of cytokine gene products shown to be regulated at the transcript level, we performed immunostaining in frozen sections from 4 representative grafts from each recipient group as published previously.15 The primary antibodies used were anti–interferon (IFN)-γ (clone R4-6A2, 1:100, Pharmingen), anti–IL-2 (clone S4B6, 1:100, Pharmingen), and anti–IL-10 (clone JES5-16E3, 1:100, Pharmingen).
Morphometric Vessel Analysis
Vascular analysis was performed in paraffin sections stained with Verhoeff's elastin as previously described.14 Microscopic images of all elastin-positive vessel cross sections were used to trace the lumen and the internal elastic lamina (NIH Image 1.6) and derive the percentage of luminal occlusion.14 Values were calculated as the mean from all captured vessels per heart and reported as mean±SEM for all grafts in each recipient group.
Leukocyte Infiltration
Immunostaining for the leukocyte common antigen CD45 (clone 30F11.1, 1:1000, 1 hour at 37°C, Pharmingen) was performed in paraffin sections from 5 representative grafts in each group. Microscopic images over the entire sagittal cross section of all grafts were captured with NIH Image 1.6 (NIH). The number of CD45-positive cells/mm2 was counted and reported as mean±SEM for all grafts in each group.
For comparisons between the average measurements of 2 groups, unpaired t tests were used. For comparison between >2 groups, ANOVA and the Bonferroni-Dunn procedure were used for post hoc testing. A probability value <0.05 was considered significant.
Intragraft Cytokine Activation
Cardiac grafts placed into STAT4 −/− recipients had reduced expression of Th1 cytokines. As shown in Figure 1A⇓, corrected transcript levels for IFN-γ (0.03±0.01 relative units [RU]) and IL-2 (0.06±0.01 RU) were significantly lower than those in grafts from wild-type control recipients (IFN-γ 0.50±0.12 RU, P<0.001; IL-2 0.60±0.11 RU, P<0.001). In grafts from wild-type control recipients, IFN-γ antigen typically localized within the thin cytoplasmic rim of mononuclear cells (Figure 1B⇓). These positive cells were diffusely distributed throughout the perivascular spaces and the myocardium. In contrast, grafts from STAT4 −/− had occasional but rare IFN-γ–positive cells. IL-2 antigen also localized to mononuclear cells (Figure 1C⇓). IL-2 was expressed in disseminated clusters of mononuclear cells throughout the entire graft cross section. In contrast, grafts from STAT4 −/− only had far fewer IL-2–positive mononuclear cells. The deficiency in Th1 cytokines was associated with modest but significant reductions in the Th2 cytokines IL-4 (0.02±0.01 RU, P<0.05) and IL-10 (0.10±0.02 RU, P<0.05).
In contrast, grafts placed into STAT6 −/− recipients had expression levels of Th2 cytokines comparable to those of wild-type controls. Corrected transcript levels for IL-4 were expressed at baseline levels in both groups (STAT6 −/− 0.09±0.02 RU, controls 0.07±0.02 RU). IL-10 transcript levels were unchanged in grafts from STAT6 −/− (0.35±0.07 RU) compared with control recipients (0.29±0.07 RU). The presence of the IL-10 gene product was confirmed by immunostaining in grafts from both groups. In both STAT6 −/− and wild-type control recipients, IL-10 antigen was present in the thin cytoplasmic rim of mononuclear cells, which were distributed throughout the perivascular myocardium (data not shown). Transcript levels for the Th1 cytokines IFN-γ (0.42±0.16 RU) and IL-2 (0.57±0.11 RU) were not significantly changed.
Hence, transplantation into STAT4-deficient recipients ablated the Th1 response compared with wild-type controls, whereas STAT6-deficient recipients had a profile of signature cytokines resembling that of wild-type controls.
Graft Vasculopathy
As shown in Figure 2⇓, grafts in STAT4 −/− recipients had less prominent vascular thickening than those in wild-type controls. Grafts from wild-type controls and STAT6-deficient recipients had circumferential thickening of the vessels with perivascular mononuclear cell infiltration. The expanded neointima was composed of cells with features of vascular smooth muscle cells and mononuclear cells. In marked contrast, grafts from STAT4-deficient recipients had only a small amount of neointimal formation, with markedly less perivascular mononuclear cell infiltration. Because of the small amount of neointimal formation in the majority of the vessels, the composition of the expanded neointima was difficult to discern.
Quantitative analysis of all elastin-positive vessels showed that grafts placed into STAT4 −/− recipients had significant reductions in frequency (24±2% of all vessels [n=113] affected) and severity (9±4% mean luminal occlusion) of cardiac allograft vasculopathy compared with those placed into wild-type controls (70±12% of all vessels [n=56], 25±6% mean luminal occlusion). In contrast, in grafts placed into STAT6 −/− recipients, the development of cardiac allograft vasculopathy was comparable to that in wild-type controls (62±8% of all vessels affected, with a mean luminal occlusion of 28±6%). Frequency and severity of allograft vasculopathy were comparable in grafts placed into C57BL/6J×129Sv wild-type controls and those placed into C57BL/6J inbred controls (61±10% of all vessels [n=101], 29±7% mean luminal occlusion). This clearly argues that the significant reduction in vascular occlusion in grafts from STAT4 −/− recipients can be attributed to the deletion of the transcription factors rather than potential differences in the genetic background. Taken together, STAT4, but not STAT6, plays an essential role in the development of cardiac allograft vasculopathy.
Mononuclear Cell Infiltration
Grafts in STAT4-deficient recipients had fewer infiltrating CD45+ mononuclear cells, as shown in the representative photographs in Figures 3A⇓ and 3C⇓. Analysis of 410 high-power fields showed that grafts placed into STAT4 −/− recipients had significantly lower numbers of graft-infiltrating CD45-positive cells (99±27 cells/mm2) compared with wild-type controls (551±168 cells/mm2, P<0.05; Figure 3B⇓).
For quantification of CD4, CD8, and Mac-1 expression to estimate the contribution of CD4- and CD8-positive lymphocytes and macrophages, we elected to measure corrected transcript levels because the resolution of these cell surface markers after immunostaining of frozen sections is not sufficient to perform quantification. Grafts placed into STAT4 −/− recipients had significantly lower transcript levels for CD4 (0.24±0.04 RU) and CD8 (0.10±0.04 RU) than those placed into wild-type controls (CD4 0.85±0.12 RU, P<0.001; CD8 0.68±0.24 RU, P<0.05). There was no significant difference in corrected transcript levels for Mac-1 (STAT4 −/− 0.35±0.02 RU, wild-type controls 0.49±0.09 RU). Taken together, there is reduced mononuclear cell infiltration in grafts from STAT4-deficient recipients, which may be related, in part, to differential recruitment of CD4- and CD8-positive lymphocytes.
Cytoadhesion Pattern
Because recruitment of Th1 lymphocytes has been shown to be regulated by P- and E-selectin cytoadhesion pathways,6 we measured intragraft transcript levels (Figure 4⇓). Corrected transcript levels for P- and E-selectin, expressed by the donor-derived endothelium, were comparable in grafts from STAT4 −/− (P-selectin 0.37±0.02 RU, E-selectin 0.20±0.05 RU) and wild-type control recipients (P-selectin 0.35±0.04 RU, E-selectin 0.33±0.03 RU). In contrast, transcript levels for P- and E-selectin glycoprotein ligands (PSGL and ESGL), expressed by recipient-derived lymphocytes, were significantly lower in grafts placed into STAT4 −/− recipients (PSGL 0.14±0.02 RU, ESGL 0.30±0.03 RU) than in those placed into wild-type controls (PSGL 0.62±0.11 RU, P<0.001; ESGL 0.71±0.17 RU, P<0.01). Hence, STAT4-deficient recipients had reduced expression of glycoprotein ligands, which are responsible for selective tissue recruitment of Th1 cells.
Here, we show that immune responses mediated by STAT4, but not STAT6, contribute to the development of cardiac allograft vasculopathy. Cardiac allografts placed into immunosuppressed recipient mice with targeted deletion of STAT4 had marked reductions in frequency and severity of vascular occlusion. The decrease in intimal thickening in the STAT4-deficient microenvironment was associated with low intragraft expression of Th1 signature cytokines (IFN-γ and IL-2), reduced expression of Th1-specific cytoadhesion markers (PSGL and ESGL), and decreased infiltration of CD45-positive cells within the graft. Hence, when present, STAT4-mediated pathways contribute to the alloimmune response that culminates in cardiac allograft vasculopathy by directing Th1-specific effector functions.
STAT4-Mediated Th1 Forces Promote Cardiac Allograft Vasculopathy
This study is the first to exploit mice with targeted deletion of STAT4 to characterize the role of Th1 cytokine programs in vivo. As opposed to single cytokine knockouts used in the past, this approach offers the advantage of presumably disrupting an entire differentiation program, resulting in deficiencies of multiple cytokines and effector pathways. In these mice, our results indicate that Th1-type immune activation may be an essential driving force in the development of chronic rejection.
These findings complement previous studies showing reduced vascular occlusion if mice with targeted deletion of the Th1 cytokine IFN-γ are used as recipients in immunosuppressed models producing chronic graft survival.13 18 However, depending on the immunosuppressive program, recipient IFN-γ deficiency may also accelerate late forms of parenchymal rejection,15 19 which supports the argument that IFN-γ may be protective. For the Th2 cytokine IL-4, targeted deletion of recipient sources did not alter survival times or development of vascular thickening in mouse transplant models with various immunosuppressive programs.15 16 20
Recently, migration studies have shown that Th1 cells are selectively recruited into inflamed sites in Th1- but not in Th2-dominated disease models by distinct cytoadhesion pathways.6 21 Only Th1 cells accumulated in sites of a delayed-type hypersensitivity reaction of the skin or arthritic joints. Th1 cell migration was blocked by antibodies against P- and E-selectin. Hence, STAT4-mediated pathways, when present, might upregulate surface expression of ESGL and PSGL (and potentially other adhesion mechanisms), thereby controlling infiltration of activated Th1 cells. In response to alloimmune stimulation, these graft-infiltrating cells would express Th1-type cytokines, initiating downstream effects promoting arteriosclerotic thickening. IFN-γ, for example, has been shown to promote cardiac allograft vasculopathy through increased expansion of vascular smooth muscle cells within the neointima.13 Alternatively, STAT4-mediated expression of Th1 cytokines might also amplify the arteriosclerotic cascade by inducing expression of ESGL and PSGL in an autocrine fashion. Studies in IFN-γ knockout recipients have shown reduced expression of leukocyte adhesion molecules associated with attenuated lesion development.18 In the present study design, we were not able to determine whether decreased PSGL and ESGL expression causes the reduced Th1 cell infiltration or whether it just reflects the reduction in glycoprotein-bearing Th1 cells. At this point, the transcriptional control of the expression of the respective glycoprotein ligands in T cells has not yet been studied. Future studies will help to dissect the interrelated roles of cytokines and cytoadhesion molecules contributing to Th1-mediated effector functions.
Cardiac Allograft Vasculopathy Develops Independently of STAT6
Evidence that STAT6 is essential for Th2 responses has been established in Th2-dominated disease models. Lymphocytes from STAT6-deficient mice infected with Nippostrongylus brasiliensis produced profoundly reduced IgE and IgG1 responses12 and failed to expel the parasite.22 STAT6-deficient mice infected with Schistosoma mansoni developed smaller hepatic granulomas associated with reduced amounts of Th2 cytokines.23 Finally, STAT6-deficient mice showed markedly reduced airway reactivity, peribronchial inflammation, and eosinophilia in allergen-induced airway models.24 25 Hence, targeted deletion of STAT6 proved to be sufficient to disrupt Th2 responses in these experimental model systems.
In contrast, in our model system, disruption of recipient sources of STAT6 did not result in any phenotypic changes in the graft vasculature or cytokine activation. As described above, IL-4 transcripts were hard to detect in grafts placed into both wild-type and STAT6-deficient recipients. Graft expression of IL-10, IFN-γ, and IL-2 was comparable in grafts from both groups. One interpretation is that cardiac allograft vasculopathy in chronically rejecting hearts develops independently of STAT6-mediated signaling pathways. This would extend previous studies in IL-4 knockout recipients showing no differences in parenchymal and vascular outcome.15 16 20 Taken together, these findings argue against a role for Th2 forces in promoting chronic rejection.
We used recipient mice with targeted gene deletion to demonstrate a significant role for STAT4-mediated Th1 forces in the pathogenesis of cardiac allograft vasculopathy. When present, STAT4 might promote the development of cardiac allograft vasculopathy by directing P- and E-selectin–mediated recruitment of activated Th1 cells into the graft. This would result in increased inflammatory activation and graft-specific secretion of the proarteriosclerotic Th1 effector cytokines IFN-γ and IL-2. In the future, this model system can be used to identify the downstream molecular pathways regulated by Th1 forces that contribute to cardiac allograft vasculopathy.
This work was supported by the NIH (HL-59578). Dr Koglin was supported by grants from the Deutsche Forschungsgemeinschaft, Bonn, Germany, and from the Deutsche Transplantationsgesellschaft. We are indebted to Dr James N. Ihle (St Jude Children's Research Hospital, Memphis, Tenn) for providing STAT4- and STAT6-knockout mice.
- Received June 28, 1999.
- Revision received August 23, 1999.
- Accepted September 9, 1999.
- Copyright © 2000 by American Heart Association
- ↵
Krieger NR, Yin DP, Fathman CG. CD4+ but not CD8+ cells are essential for allorejection. J Exp Med. 1996;184:2013–2018.
- ↵
Räisänen-Sokolowski A, Glysing-Jensen T, Mottram PL, Russell ME. Sustained anti-CD4/CD8 treatment blocks inflammatory activation and intimal thickening in mouse heart allografts. Arterioscler Thromb Vasc Biol. 1997;17:2115–2122.
- ↵
Shi C, Lee WS, He Q, Zhang D, Fletcher DL Jr, Newell JB, Haber E. Immunologic basis of transplant-associated arteriosclerosis. Proc Natl Acad Sci U S A. 1996;93:4051–4056.
- ↵
- ↵
- ↵
- ↵
Sallusto F, Lenig D, Mackay CR, Lanzavecchia A. Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes. J Exp Med. 1998;187:875–883.
- ↵
- ↵
- ↵
- ↵
- ↵
- ↵
- ↵
Koglin J, Glysing-Jensen T, Mudgett JS, Russell ME. Exacerbated transplant arteriosclerosis in inducible nitric oxide–deficient mice. Circulation. 1998;97:2059–2065.
- ↵
- ↵
Mottram PL, Räisänen-Sokolowski A, Glysing-Jensen T, Stein-Oakley AN, Russell ME. Cardiac allografts from IL-4 knockout recipients: assessment of transplant arteriosclerosis and peripheral tolerance. J Immunol. 1998;161:602–609.
- ↵
Russell ME, Adams DH, Wyner LR, Yamashita Y, Halnon NJ, Karnovsky MJ. Early and persistent induction of monocyte chemoattractant protein 1 in rat cardiac allografts. Proc Natl Acad Sci U S A. 1993;90:6086–6090.
- ↵
- ↵
Konieczny BT, Dai ZH, Elwood ET, Saleem S, Linsley PS, Baddoura FK, Larsen CP, Pearson TC, Lakkis FG. IFN-gamma is critical for long-term allograft survival induced by blocking the CD28 and CD40 ligand T cell costimulation pathways. J Immunol. 1998;160:2059–2064.
- ↵
- ↵
Borges E, Tietz W, Steegmaier M, Moll T, Hallmann R, Hamann A, Vestweber D. P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin. J Exp Med. 1997;185:573–578.
- ↵
- ↵
Kaplan MH, Whitfield JR, Boros DL, Grusby MJ. Th2 cells are required for the Schistosoma mansoni egg-induced granulomatous response. J Immunol. 1998;160:1850–1856.
- ↵
Akimoto T, Numata F, Tamura M, Takata Y, Higashida N, Takashi T, Takeda K, Akira S. Abrogation of bronchial eosinophilic inflammation and airway hyperreactivity in signal transducers and activators of transcription (Stat)6-deficient mice. J Exp Med. 1998;187:1537–1542.
- ↵
Kuperman D, Schofield B, Willskarp M, Grusby MJ. Signal transducer and activator of transcription factor 6 (Stat6)-deficient mice are protected from antigen-induced airway hyperresponsiveness and mucus production. J Exp Med. 1998;187:939–948.
This Issue
Jump to
Article Tools
- Attenuated Cardiac Allograft Vasculopathy in Mice With Targeted Deletion of the Transcription Factor STAT4Jörg Koglin, Troels Glysing-Jensen, Silpa Gadiraju and Mary E. RussellCirculation. 2000;101:1034-1039, originally published March 7, 2000https://doi.org/10.1161/01.CIR.101.9.1034
Citation Manager Formats


text: e-Kranti: National e-Governance Plan 2.0 Next Wave in e-Governance Transforming e-Governance for Transforming Governance Vinay Thakur Director, NeGD Department of Electronics and Information Technology (DeitY) [email protected] Agenda Overview of NeGP Status of DeitY's Core Infrastructure Projects SWOT Analysis of NeGP Need for e-Kranti (NeGP 2.0) Principles of e-Kranti Way Forward National e-Governance Plan (NeGP): Vision "Make all Government services accessible to the COMMON MAN IN HIS LOCALITY, through Common Service Delivery Outlets and ensure EFFICIENCY, TRANSPARENCY & RELIABILITY of such services at AFFORDABLE COSTS to realise the BASIC NEEDS of the common man" May 2006 Core Infrastructure under NeGP State Wide Area Network 40 35 30 25 20 15 10 5 0 36 33 31 30 State Data Center 25 30 20 15 12 0 2009-10 2010-11 2011-12 2012-13 2013-14 2014-15* 2010-11 No. of CSCs Rolled Out Connectivity 1,60,000 1,35,598 1,20,651 1,40,000 1,20,000 95,710 1,00,000 80,669 80,000 99247 85,928 69,574 57,482 60,000 43,464 40,000 27,200 10,350 4,000 0 2007-08 16 2008-09 2009-10 2010-11 2011-12 2011-12 2012-13 2013-14 2014-15* SSDG and State Portal Common Service Centers 20,000 23 10 7 2008-09 21 28 Jun-2014 30 25 20 15 10 5 0 25 20 11 1 2010-11 4 2011-12 2012-13 2013-14 2014-15* * Target Strengths Weaknesses General Awareness on eGovernance 24 out of 31 MMPs gone live Basic IT Infrastructure available Significant increase in political support Catalyzed movement towards citizen right on time bound delivery of services Supplemented various eGov projects Opportunities Huge advancements in the Technology Advent of the Cloud New business models Capacity Building Radical process re-engineering Strong international presence Lack of attainment in desired impact Significant time overruns Weak Standards and interoperability Low degree of process-reengineering Lack of mission approach on implementation Weak monitoring & evaluation system Problem of last mile connectivity Sub optimal use of Core IT Infra NeGP Threats Losing appeal for Transformation Obsolete or inefficient eGov Projects A large number of islands of IT activity Competition from other countries India could lose the opportunity to leapfrog in the quality and nature of citizen services offered through eGov Why NeGP 2.0? (1/2) o For enhancing portfolio of citizen centric services o Current Portfolio of MMPs does not cover all citizen centric services o Long gestation period of MMPs (Health, Education, e-Posts) o Desirability of optimum usage of core infrastructure o SDC – 23/33, SWAN – 33/36, SSDG – 20/36, CSCs – 135,598 o Fully established MSDG, NSDG and Payment Gateway o Current average utilization of assets is about 50 % o For rapid replication and integration of eGov applications o Lack of integrated services o Lack of end to end automation o Lack of interoperability among exiting eGov applications o Lack of replication of successful eGov applications across States and UTs Why NeGP 2.0? (2/2) o Need to exploit Emerging Technologies o Cloud o Mobile Platform- Smart phones, Tablets o In-Memory Database o Software Defined Network (SDN) o Big Data Analytics o Avoid risk of obsolescence o Need for introducing more agile implementation models Need for Redefining NeGP o Incremental approach Vs. Transformational approach o Consequence of SWOT Analysis o New Image o New Priorities o Global Leadership NeGP 2.0 (e-Kranti) Principles o Transformation and not Translation o Integrated Services and not Individual Services o GPR to be mandatory in every MMP o Infrastructure on Demand o Cloud by Default o Mobile First o Fast Tracking Approvals o Mandating Standards and Protocols NeGP 2.0 (e-Kranti) Institutions and Instruments o National e-Governance Academy o e-Governance Knowledge Portal o Create e-Governance Impact Index o Effective use of Social Media Implementation & Delivery o Transforming the Delivery Channels o Awareness and Communication o Introduce New Business Models o Exporting eGov Status update on e-Kranti o Discussed in PM's Committee on NeGP on 1st July, 2013 o Discussed in Apex Committee on NeGP on 5th November, 2013 o Concept paper circulated to all Central Ministries/Departments and all States/UTs for comments o Discussed in Council of Mission Leaders on 14th March' 2014 o Discussed in Apex Committee on NeGP on 18th March' 2014 o 10 New MMPs approved in principle by the Apex Committee o Revised Cabinet Note to be circulated New MMPs Sl. No. 1 2 3 4 5 New MMPs Owner Department Key Components Parliament of India, Bills, Gazette notifications, Budget, Lok-Sabha Secretariat Parliamentary Questions database, Parliamentary proceedings, Publications Parliament of India, As above for State Legislatures e-Vidhaan Lok-Sabha Secretariat Strengthening Banking & Insurance Financial Inclusion Financial Services services in the rural areas through strategic use of ICT Roads and M/o Road Transport & Integrated citizen centric services Highways related to roads and highways Highways e-Sansad Information System (RAHI) Agriculture 2.0 D/o Agriculture Sector specific services for Horticulture and Fisheries, Governance & citizencentric services for Co-operatives and Fertilizer testing labs New MMPs Sl. No. New MMPs Owner Department M/o Earth Sciences 6 NGIS 7 Rural Development D/o Rural Key Components Integrated GIS Platform A portfolio of rural development services including NREGA Online Benefit Schemes, Integrated eServices for NGOs Development M/o Social Justice and Empowerment as the leader and other welfare departments as coowners M/o Women and Integrated Child Development Scheme, Child Development Integration with Health MMP 8 Social Benefits 9 Women and Child Development 10 MHA Common IT Roadmap for Para Military Forces Way Forward o DPR has been prepared, incorporating major components like New Portfolio of Projects Mandating transformational BPR, Cloud by Default, Mobile First and Standards National e-Governance Academy National Institute for e-Governance Standards e-Governance Impact Index NII Fast-track approval procedures o Revised Cabinet Note to be circulated SDC Implementation Status Across India J&K HP Punjab Chandigarh Arunachal Pradesh Uttarakhand Delhi Haryana Sikkim Nagaland UP Assam Rajasthan Manipur Bihar MP Gujarat JHD CHH Orissa Dadra Daman & Diu Mizoram WB Tripura Meghalaya Maharashtra M aharas htra SDC - Go Live AP Goa A n d a TN Pondicherry m a n Tamil Kerala Lakshadweep Nadu 23 SDC Operational 1 Apr - May'14 1 Dec'14 4 Feb-Mar'15 4 Jul-Aug'15 2 Opted Out BACK SWAN: Implementation Status across India –July 2014 J&K Himachal Pradesh CHD HP Arunachal Pradesh Uttarakhand Punjab Punjab Haryana Haryana Sikkim Delhi UttarUP Pradesh Assam Rajasthan Bihar Bihar Gujarat Gujarat JHD Jharkhand MP MNP MGH WB West Bengal TRIPURA NG MZR CHH Daman and Diu Orissa Orissa Dadar and Nagar Haveli 33 Maharashtra Maharashtra AP Goa* Andhra Pradesh Karnataka Pondicherry Lakshadweep 1 Re-bid Process in progress 1 LoI to be issued Andman Karnataka SWAN operational BACK TN Kerala Kerala Tamil Nadu * Implemented under State Scheme SSDG Implementation Status across India (July, 2014) BACK CSC: Implementation Status (30th June, 2014) BACK


text:
Goldander Blackenrock is a svirfneblin gnome who lives in the Underdark near the drow city of Ust Natha.
When you reach the Underdark in Chapter 5, you will need to speak with Goldander in order to reach the surface. You can either kill him and collect the Light Gem from his dead body or collect it as a reward for completing the task he gives you.


text:
We still have 11 turkeys. Here they are a week before I left for the cottage, they were quickly getting too big for the small enclosure next to the turkeys, so I moved them to the large turkey coop (aka temporary goat birthing room).
It seemed like within days of moving them, they doubled in size. Now they are HUGE, and they make that wonderful gobbling sound (they are ready to speak to Carrie, the turkey whisperer).
Once I got home from the cottage, I set up their outside enslosure using old calf panels held together with ties. They LOVE being outside and they love eating grass and weeds, and in no time they had devoured everything green in their small yard. A week earlier, that area was full of uncut grass, dandelions, clover and plantain weeds.
We are going to have to extend their yard, in the meantime, they are enjoying garden clippings and table scraps.


text:
Please login above. Note that in most cases, your username is not the same as your email address.
Need help?moc.pmaib@troppusbew
Check out our press releases below for the latest Biamp announcements.
Sep 11, 2009
"We're shipping RED-1 earlier than expected because of the high demand for the product," said Steve Metzger, VP of Business Development, Biamp Systems. "We expect RED-1's demand to continue to grow as more integrators and end-users begin using the remote control."
The new remote control, which can be installed and configured to fit the needs of any application, features an easy-to-read OLED screen and a capacitive touch wheel that gives users the option of adjusting or initiating up to 32 selectable system volumes and actions. Additionally, the control functions are pre-programmed into the Audia® and Nexia® system software, making RED-1 a natural fit for any Biamp installation.
"RED-1 resulted from a strong demand by our customers for a remote control that was attractive, versatile and intuitive," said Matt Czyzewski, VP of Engineering, Biamp Systems. "This product achieves all of those things. We believe RED-1 extends the same conceptual qualities of flexibility and power that our customers have come to expect from AudiaFLEX and Nexia directly to the user interface."
RED-1 connects through standard CAT-5/6/7 cabling and is powered over Ethernet, eliminating the need for custom cabling and local power sources. Additionally, RED-1 surface mounts to any wall, which enables a fast and simple installation.
To ensure your shipment of RED-1, contact your Biamp Systems representative. Or, to learn more, visit www.biamp.com/products/audia/controls.aspx or www.biamp.com/products/nexia/controls.aspx.
9300 SW Gemini Drive
Beaverton, OR 97008 USA
US Phone:
1 800 826 1457
International Phone:
+1 503 641 7287
Biamp Support:
Devio Support:
US Phone:
International Phone:
Choose Your Region


text:
Campus Map
At the Center for Weight Loss, surgery is only part of the care you receive. We create a personalized, comprehensive program that addresses your physical, nutritional and emotional needs. Each member of our team is committed to your success and you'll receive the same high-level, one-on-one care and support before, during and after your surgery.
We believe that obesity is a serious disease. Obesity is known to be the primary cause for many medical problems, such as diabetes, high blood pressure, obstructive sleep apnea, asthma, heart disease and many cancers—each of which has significant health consequences. Yet if the disease is treated, many of those consequences can be dramatically lessened or avoided altogether.
Fortunately, there are now more options to help those suffering from obesity. Dr. Lautz and his team will work with you to find the solution that suits your preferences and lifestyle. We believe that the relationship between patient and surgeon is the cornerstone of treatment, and we work hard to establish it at the first clinic visit.
The Center for Weight Loss is credentialed by the American College of Surgeons as a Level 1A Bariatric Surgery Center of Excellence, the same level of designation as major city hospitals. Our newly designed center is one of the only clinics in the region specifically designed for obese and overweight patients—from the waiting room chairs to special clinical equipment to private check-out areas. The center also offers free on-site parking with a nearby, street-level entrance to accommodate those with mobility issues.


text:
Bollywood is known to splurge on their movies and when it comes to actresses, the industry does not shy away. There are a number of actresses in Bollywood, but some of them have made their mark in the industry and this means they demand higher salaries for the movies they act in. Producers don't mind shelling out this kind of money considering that these leading ladies have a huge fan following and apart from drop dead gorgeous looks, they have what it takes to be an actress. Here are ten highest paid actresses in Bollywood.
Bollywood is known to splurge on their movies and when it comes to actresses, the industry does not shy away. There are a number of actresses in Bollywood, but some of them have made their mark in the industry and this means they demand higher salaries for the movies they act in. Producers don't mind shelling out this kind of money considering that these leading ladies have a huge fan following and apart from drop dead gorgeous looks, they have what it takes to be an actress. Here are ten highest paid actresses in Bollywood.
10. Vidya Balan
Salary Per Movie: 8 Crores
Vidya is one actress who proved that you don't need to be a size 'Zero' to make your mark in the industry. She is one of the most talented actresses in Bollywood who is known to portray some amazing roles including The Dirty Picture, Kahaani and Ishqiya. Her last movie was Hamari Adhuri Kahani where she was paid Rs. 8 Crores.
9. Aishwarya Rai Bachchan
Salary Per Movie: 10 Crores
Aishwarya is one of those actresses who still manage to mesmerize the audience when she comes on screen. She has always been one of the most loved stars in Bollywood and while she might have taken a break so she could spend more time with her daughter, Aish came back with a bang in the movie 'Jazbaa' and charged a whopping 10 crores to play the lead.
8. Kangana Ranaut
Salary Per Movie – 11 Crores
Kangana Ranaut recently hiked her fees after the stupendous success of Tanu Weds Manu Returns and Queen. She has grabbed a lot of big projects in the coming years and this will certainly put her above everyone else in this list. Her salary of 11 crores per movie will increase with time. Her upcoming movies include Simran, Rangoon and Rani Laxmi Bai. This will certainly increase her demand in Bollywood.
7. Sonakshi Sinha
Salary Per Movie- 10.5 Crores
Sonakshi has slowly but surely gained immense popularity among the fans and among her Bollywood colleagues too. Sonakshi's charming smile and powerful acting makes her get noticed in any kind of movie. A case in example is Dabangg that was a male dominated movie and Sonakshi still impressed everyone with her acting. She acts in about 3 or 4 movies a year and earns approximately 6 crores per movie. Her popularity among her Bollywood colleagues has also resulted in her making special appearance in songs. It is said that she earned 10.5 crore from "Tevar".
6. Anushka Sharma
Salary Per Movie: 11 Crores
Anushka is one amazing actress who pulls off versatile roles with ease. She has been part of the industry for a long time now, and although she might have missed the mark with a few movies, she is still one of the most popular actresses on screen. Her last movie 'Sultan' gained her a lot of popularity, both negative as well as positive, but she knows how to make the best of her career and it looks like she is following the right path this year.
5. Kajol
Salary Per Movie: 12.5 Crores
Kajol is one of the most loved actresses of all time. She has been a very popular star since the 90's and although she was on a break post marriage, she did a quite a few movies after that. Kajol fans still love to see her on screen and she still manages to steal the hearts of people all over the world with her acting skills and her beautiful personality. Kajol was last seen in 'Dilwale' where she was paid Rs. 12.5 Crores.
4. Sonam Kapoor
Salary Per Movie: 14 Crores
Sonam is one the daughter of Anil Kapoor who is one of the most talented actors in the industry. There's no denying that Sonam is an amazing actress who is very versatile. She managed to pull off some amazing roles such as 'Neerja' where she was highly appreciated. While her career might not have taken off the right way, she has surely made a mark for herself in the industry.
3. Priyanka Chopra
Salary Per Movie – 18.5 Crores
While Priyanka is busy jet setting between India and the US to shoot for Bollywood movies and her American show, she manages to pull it off with ease. She gets paid a nominal salary of 18.5 crores per movie which is a lot lower than what actresses in the US charge. Priyanka's eventual goal is to become popular in Hollywood and gain major projects over there. This will definitely increase her value in Bollywood as well. She is currently shooting for a serial in US and is also reportedly part of the new Baywatch series.
2. Kareena Kapoor Khan
Salary Per Movie: 20 Crores
Kareena might be pregnant, but this does not take away from the fact that this is one of the only actresses that managed to remain popular post her marriage. She promises to come back post her baby, and her fans are eager for her to sign her next movie. She was last seen in 'Udta Punjab' where she reportedly charged Rs. 20 crores.
1. Deepika Padukone
Salary Per Movie – 25.5 Crores
Deepika has been known to act in various big budget movies and her success in Bollywood is unparalleled. She is considered one of the most talented actresses in Bollywood today and that's the reason we see her regularly doing three movies a year. Her recent blockbuster 'Bajirao Mastani' certainly put her above everyone else as far as demand in Bollywood and salary per movie is concerned. She is also one of the most loved actresses in Bollywood who is also trying her luck in Hollywood.
- Fascinated
- Happy
- Sad
- Angry
- Bored
- Afraid


text:
Carson-Dellosa Moose & Friends Border
Item #: 901-140972OSA
Model #: 108159
Brand: Carson-Dellosa Publishing
Not yet rated.Be the first to Write a Review!
$4.49 Each
The Carson-Dellosa Publishing 108159 Moose & Friends Border includes 12 strips that are sufficient for decorating any standard-sized window or bulletin board. Ensure that your classroom gives off a positive vibe and motivates children to learn by putting up the Carson-Dellosa Publishing Moose and Friends Borders. As they feature interesting abstract concentric designs in bright colors like blue, brown, green, red, orange and purple, they will enhance the decor of the classroom. These bulletin board borders can also be used on windows and lockers.
- Moose and Friends borders for revamping the interiors of prekindergarten - 8th grade classrooms
- Strips joined together will form a 36' long border
- Dimensions: 36"L x 3"W each
- Colorful design with Straight edges for a neat and continuous look
- Pack of 12
- Adds style to any class creation, bulletin board, window, locker, or anywhere a colorful design is needed
- Perfect to use alone or in layers, it has straight edges for easy use and continuity of design
- Set includes 12 strips measuring 3 ft. x 3" for a total of 36 feet!
|Border & Trim Type : Borders/Trim
|Kids Theme : Colors
|School Grade : Multi-Grade
|Pack Size (Each) : 12
|Length (ft.) : 3
|Design and Pattern : Moose & Friends
|Length in Inches : 36
|True Color : Multicolor
|Width in Inches : 36
|Brand : CARSON-DELLOSA PUBLISHING
|Weight : 1.00 lbs. per Each


text:
San Diego, CAUSA
June 7, 2009
No introduction yet
Help support your Meetup
This meetup is for anyone/everyone that is interested in the local San Diego music scene, which would include bands, venues, promoters, bookers, movie soundtracks, fans, musicians looking to be in bands, etc. We will also highlight music venues for all genres of music including unique places. Please share any places that you are aware of so we can all enjoy! The point of the meetup would be to create a big networking event for any facet of the local music scene and venues that promote the music arts. If you have a company or product that would appeal to our members contact Deepak for details. We are accepting voluntary donations of $1+ to aid San Diego charities. This will donated at the end of every month to the San Diego Wildfire 2007 charities. Check the San Diego Wildfires blog for info: http://wildfireblog.wordpress.com/ Thanks for your generosity!!! ******************** Please support our SPONSORS:
MyCouponGurus : http://www.MyCouponGurus.com
San Diego Vips : http://www.sandiegovips.com
San Diego Real Estate : http://www.HelpUSellAward.com
Online Dating : http://onlinedatingpromotions.com/
13,452 awesome people
2,229 Beach Jammers
14,899 Hikers
2,540 40+ Singles Party Goers
10,639 Fun Diegans
4,837 Fun Healthy People
Or sign up with email
Already a member? Log in.
Meetup members, Log in
By clicking "Sign up" or "Sign up using Facebook", you confirm that you accept our Terms of Service & Privacy Policy


text:
Johanna lived and worked as a Production Designer in Los Angeles for many years, after having attended a Master Degree Program in Production Design for Film at the American Film Institute. Later on she moved back to Stockholm and continued her work full time. She is very talented and also very organized in her work and no project is too small or too big for her. Johanna works with handmade construction drawings and illustrations, and is trained in color theory. She's a master in all american style and she's comfortable with working in international productions. When you work with Johanna, you can expect a perfect result and a great energy. She's known amongst colleagues and her team for being the ultimate leader.
Apoteket, Alladin, All State, Bilia, Bon Aqua, Brother, Cesar, Coca Cola, COOP, Cubus, Eniro, Eon, Expressen, Felix, Fleurop, Grandiosa, Hemtex, Hyresgästföreningen, Ikea, Jägermeister, Kalles Kaviar, Kopparberg, Max Hamburgare, Molson Beer, Morasitas, Neuf, Nokia, Norwegian, Pampers, Posten, SAS, Soduku, Systembolaget, Tele2, Telenor, Tine Rivetost, Unicef, Vaasan, Volkswagen, Svensk Fastighetsförmedling
Marc Webb, Josef Fares, Felix Herngren, Thomas Skoging, Axel Laubscher, Sebastian Reed, Owen Harris, Henrik Sundgren, Sebastian Hedin, Jörgen Lööf, Emil Möller, Jesper Kouthouft, Jesper Hiro, Jesper Ericstam, Tryan Geore, Liz Friedlander, Nzinga Stewart, Mårlind & Stein, Ensrettet, Robert Nylund, Markus Johnson, Marcus Svanberg, Henrik von Sydow, Jacob Estes
FLX, Atmosfär, Acne, Camp David, B-Reel, Outsider, Social Club, Kamisol, Fido Film, Flodellfilm, DNA, Palladium
Green Day, Good Charlotte, Mary Mary, Puddle of Mud, K-Ci & JoJo, Syleena and Busta Rymes, Michelle Ndegocello, LMNT
Guldägget 2012 together with FLX - Best Commercial (Apoteket 2012)
Best Art Direction in Germany (Jägermeister)


text:
Recently Added ×
You have no items in your shopping cart.
* Required Fields
A simply beautiful floral lace Triumph wired bra. Cut with a pretty scalloped edge, with a small cut-out centre. The fully underwired cups ensures comfort and support through out the day.
Bra Style - Full Cup
Padding - Non Padded
Underwiring - Wired
Material - 77% Polyamide, 17% Elastane, 6% Cotton
Washing Instructions - Hand Wash
Colour - White
We offer a wide range of delivery options to over 200 countries, with same day dispatch until 4pm.
See our full delivery policy for further information and for detailed information on the delivery options available.
Free - Tracked Delivery is available on all UK Orders (2-3 Working Days)
£3.95 - Next Day Delivery is available on all Mainland UK Orders placed before 4pm (Next Working Day)
£6.00 - Next Day Delivery to Non Mainland UK Postcodes (Not available for all non-mainland postcodes)
Exact services available for your delivery address will be shown at checkout
International delivery is available Worldide for £6.95 and typically takes between 4-14 days dependent on destination
Products can be returned within 30 days of delivery using our free returns service for an exchange or refund.
See our full returns policy for further information and detailed information on returns and exchanges