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Liam Hemsworth or Zac Efron?
What kind of a question is that? I'll have them both. After all, when presented with two hotties, one doesn't simply settle for one. And turns out Maika Monroe also believes in something similar.
The 'Independence Day: Resurgence' star recently made an appearance at the Young Hollywood studio with Audrey Cleo. Maika who has done movies with both Liam, who plays her love interest in 'Independence Day: Resurgence' and Zac Efron in 'At Any Price' was asked who's the better kisser between Zac and Liam?
And here's how Maika decided to answer this very difficult question. Oh! Such dilemma,
"Gosh! I know you're really throwing me on this but I don't know. I don't know. I couldn't tell you. Yeah, we'll call it a tie."
Now as I've said before it's more than difficult to choose between two exquisite human beings like Zac and Liam but given the fact that she actually was linked to Liam, she could've chosen Liam as the better kisser! Don't you remember Maika and Liam dating? It all happened after Liam and Miley broke up, now that they're back together again. Liam was rumored to be together with Maika since affections had sparked between them while filming for Independence Day: Resurgence. But that didn't last long since he's back with Miley again. Maybe Maika could've said Zac, just to roast Liam but oh! Maika so modest! Wait! Maika was even linked to Zac in 2013!
Now aside from rumors and speculations, Maika's personal, especially her romantic life has never made it to the media. Aside from Zac and Liam, she's also been linked to Taylor Lautner. WOW! That's quite a score, Maika. All the hot A-listers. However, the real mystery here is who is Maika Monroe's real boyfriend or is she more into kiteboarding than guys? Well, that's not a mystery anymore, the 23-year-old Maika Monroe is dating actor Boyd Holbrook.


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I twittered that I think I might need to lay off red wine pretty much forever, as depressing as that is. Last night, as I sat around the kitchen table with the girls, it just tasted SO good! But it always makes me mean and groggy the next day...so be forewarned. :)
The concert Friday was so much fun, and my audition Saturday went well, but now here am in a place I haven't been in a while-- wondering what to work on next. I should get an early start on Mimi, but I'd like to take a break from Boheme for a month or so. So, my teacher, ever the intuitive listener, great confidant, and ultimate encourager, who knows me so well, suggested I look at Rusalka. If you had asked me about that a year ago, I would have said it's crazy to sing that if you're not Renee Fleming-- and I'm not. Obviously. But listening to it, it's just too heavenly to refuse. God knows where I'm going to find someone to teach me the Czech, but in the face of that gorgeous music, it seems like only a minor detail. (Insert Czech people throwing shoes at my head) Gotta love Dvorak melody.
In other news, I applied for an audition in New York that, upon doing some research, I'm pretty sure is a scam. Thank god I hadn't bought a train ticket or anything, or spent hundreds of dollars. I suppose we singers (sopranos, for sure) are easy targets. Thanks for exploiting us and our dedication to our calling, jerks. **this is the red wine talking** But I figured it out before I paid you the $40 fee...so that's one trap I didn't fall into. I missed the Preakness for a lame audition experience, and you can bet I won't ruin a trip to New York for one.
Happy Tuesday!

subset: commoncrawl


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This is a layout of three of my grandaughters playing in the park. I used Making Memories patterned paper, black cardstock, Prima felt, Basic Grey chipboard a journal spot made with my slice, and I hand doodled. I'm not sure who makes the alpha stickers.
I just love all the chipboard accent pkgs from Basic Grey.


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Staying at The Abelia House was in incredible experience. It was a little bit out of town, but we had a car, so it was not a problem. Being off the be...Hotels.com に掲載されている 6 件の口コミをすべて見る
walking distance to everything. friendly,clean, convenient. highly recommendHotels.com に掲載されている 174 件の口コミをすべて見る
Stayed for two nights in a studio with a pool. It's nice but missing the final touches for the price bracket it is in. Overall it was clean but a bit ...Hotels.com に掲載されている 33 件の口コミをすべて見る
It's was a great quiet place to stay, very clean and close to everything in Byron bay, they should definitely add mirrors to the rooms in addition to ...Hotels.com に掲載されている 52 件の口コミをすべて見る
We had a wonderful stay. The room was very clean and comfortable.Hotels.com に掲載されている 27 件の口コミをすべて見る


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Neuro Pro 3 now offers a clean modern responsive WordPress theme featuring drag and drop theme options. Neuro Pro offers the same advanced functionality as other CyberChimps WordPress themes including a responsive design that responds automatically to mobile devices such as the iPhone, iPad, and Android. Neuro Pro now features 5 beautiful skins to select from, as well as drag and drop elements such as the responsive iFeature Slider, image carousel, Twitter bar, widgetized boxes, and callout section. All of which can be used on a per-page basis.
- 5 clean beautiful responsive skins to select from.
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- Theme customizer support, post formats support, translation ready, powerful blog settings, typography options including Google Fonts, widgetized sidebars and footer.
- Powered by WordPress, Twitter Bootstrap, HTML5, and CSS3.


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TransCanada Files Keystone XL Route Application In Nebraska
Feb 16 (Reuters) - TransCanada Corp, Canada's No. 2 pipeline operator, filed an application with Nebraska authorities to route its Keystone XL pipeline through the state, after U.S. President Donald Trump cleared the way for the project last month.
In November 2015, the pipeline company withdrew the route application it had made to the Nebraska Public Service Commission, after then President Barack Obama denied a permit for the controversial project over environmental concerns.
The 1,179-mile (1,900-km) Keystone XL is meant to ship 830,000 barrels per day of mainly oil sands crude from Alberta to Nebraska and on to the U.S. Gulf Coast.
Opposition in Nebraska from environmentalists and some landowners concerned about oil spills had been among several major hurdles facing the Keystone XL project.
"The (Nebraska Public Service Commission) process is the clearest path to achieving route certainty for the project in Nebraska and is expected to conclude in 2017," TransCanada said on Thursday.
The line's route through Nebraska has been the subject of a court case in the state over whether former Governor Dave Heineman was entitled to approve the route. (reut.rs/2jbx05V)
A Nebraska Supreme Court decision in 2015 ruled in support of the pipeline, but a number of Nebraskan landowners filed suits against TransCanada alleging the project violated the state's constitution. (reut.rs/2kpZzh1)
"Keystone XL is and always will be all risk and no reward," said Jane Kleeb, President of the Bold Alliance, an activist network opposing the pipeline.
TransCanada also reported a better-than-expected quarterly profit on Thursday.
The company said earnings from its U.S. natural gas pipeline business rose more than four-fold to C$416 million, due to its $13 billion acquisition of Columbia Pipeline Group in July.
The company's net loss attributable to shareholders narrowed to C$358 million, or 43 Canadian cents per share, in the fourth quarter ended Dec. 31, from C$2.46 billion, or C$3.47 per share, a year earlier.
The latest quarter included charges of about C$1 billion, while the year-earlier quarter had impairment charges of about C$2.9 billion related to the Keystone XL pipeline.
Excluding items, the company earned 75 Canadian cents per share, beating the average analysts' estimate of 72 Canadian cents, according to Thomson Reuters I/B/E/S.
Revenue rose 26.9 percent to C$3.62 billion in the quarter. Analysts were expecting a revenue of C$3.50 billion.
($1 = 1.3025 Canadian dollars)
(Reporting by Swetha Gopinath and Vishaka George in Bengaluru; Editing by Anil D'Silva and Shounak Dasgupta)
Generated by readers, the comments included herein do not reflect the views and opinions of Rigzone. All comments are subject to editorial review. Off-topic, inappropriate or insulting comments will be removed.
- Petronas Says Involved In Proposal To Build Gas Pipeline Extension (Mar 23)
- Keystone Saga Takes Toll on Alberta as Oil Holdups Worsen (Feb 28)
- TransCanada Expands Gas Pipelines With Keystone XL in Limbo (Feb 15)


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UND President Mark Kennedy invites campus & community to Eye of the Hawk Lecture Series event
Diversity and Inclusion in 2017: Our Next Round of Work
Rev. Jamie Washington M.Div., Ph.D.
January 30, 2017 | 5:30 p.m.
Gorecki Alumni Center, University Avenue, Grand Forks
We live in a world where people from diverse backgrounds, experiences, social identities and ideologies must live, work, learn and grow together. Many times we face acts of intolerance or injustice and we just keep fighting as if there has been no impact. We get up every day and make sure others are well and don't take time to take care of ourselves. This session will address the following and introduce tools and key concepts for engaging a world where all people matter and we can build the capacity to see the world differently without doing each other unintended harm.
- Provide an opportunity for participants to share their feelings about how they have been impacted by racial intolerance.
- Create the space to deepen campus community members' capacity to respectfully engage conversation about race dynamics.
- Promote individual and community healing.
President Mark and First Lady Debbie Kennedy developed the Eye of the Hawk Lecture Series as a way to bring the UND community together to discuss important topics with preeminent experts. The UND College of Arts & Sciences Eye of the Hawk Lecture is sponsored by Rick and Jody Burgum.


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(Reuters) - The U.S. government is set to publish sweeping new rules on Tuesday that will require chain restaurants to disclose calorie counts on menus, establishing a national standard that pre-empts the current patchwork of state laws and applies to restaurant chains with 20 or more outlets.
The new standards, which are part of the 2010 Affordable Care Act, were announced by the Food and Drug Administration and also apply to large vending machine operators.
Read more


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The 813 bus service that serves the villages Bramcote, Shilton, Bulkington and Nuneaton schools crashed into the wall of Bedworth Leisure Centre at 12.05pm today (Wednesday).
On hitting the wall, the bus caused swimming pool water to come flooding out. Phoenix Coaches operates the service. It is not yet known whether there were any children on board during the time of the crash.
"At 12.05pm Bedworth received a call to a bus that had a collision in to the side of Bedworth Leisure Centre and water from the pool was flooding out," a spokesman for the Warwickshire Fire and Rescue Service said.
"Crews cordoned off the area with help of the police and an ambulance was requested for the driver of the coach as a precaution."
"Crews have made effect to reduce the flooding and structural engineers are working to make the building safe."
Phoenix Coaches ensure that all of their 'uniformed drivers are CRB checked'. A spokesperson for Phoenix Coaches confirmed that no one had been injured in the crash.
Meanwhile, Bedworth Leisure Centre's website released the following statement:
"Due to unforeseen circumstances Bedworth Leisure Centre will be closed until further notice. We will endeavour to update once further information is available and when the centre is scheduled to reopen."
"We apologise for any inconvenience this may cause," they added.


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Construction spending up for seventh straight month to three year high in October
Construction cranes in Greater Boston and nationwide are having an impact as all major segments of building spending increased in October, bringing the total to a 37-month high at an annualized rate of $872 billion, according to an analysis of new federal data by the Associated General Contractors of America.
"Widespread gains in spending in October, along with hefty upward revisions to estimates for the previous two months, show that construction has finally come out of its long slump," said Kenneth Simonson, the association's chief economist, in a statement.
Construction spending rose for the seventh-consecutive month in October, up 9.6 percent from October 2011, bringing the total to the highest level since September 2009. Private residential spending reached the highest level since November 2008, increasing by 21 percent during the past year. Private nonresidential construction edged up 11 percent compared to one year ago. Public construction rose 0.8 percent in October, but slipped 1 percent from the same period last year.
Within the private sector, the three residential sectors set multiyear peaks. New single-family construction rose to the highest mark since November 2008, climbing 3.6 percent for the month and 29 percent over 12 months. New multifamily construction hit a three-year high, soaring 6.2 percent for the month and 53 percent since October 2011. Improvements to existing residential structures, a category likely to grow further once reconstruction from Hurricane Sandy is included, climbed 1.8 percent in October and 8.9 percent over the year to a five-year high.
Among private nonresidential categories, power construction, which includes oil, gas and other energy projects, rose 1.6 percent for the month and 19 percent over 12 months. Manufacturing construction decreased 2.5 percent in October, but was up by 3.6 percent from the same month last year. Commercial construction, comprising retail, warehouse and farm structures, rose 1.2 percent in October and 9.5 percent over 12 months.
Public construction achieved the highest spending level of the year. Highway and street construction spending, the largest public segment, dropped 2.4 percent in October and 5.0 percent year-over-year, while educational construction spending gained 0.9 percent for the month but fell 2.8 percent from year-ago levels.
But association officials warned that the "fiscal cliff" imperils future improvement in public and private spending.
Stephen Sandherr, the association's CEO, urged Congress and the White House to reach a quick and lasting agreement on federal spending, taxes and the debt ceiling. "Without greater certainty on tax rates and federal spending levels, private and public construction will quickly reverse recent gains, throwing newly hired workers back into unemployment," said Sandherr in a statement.


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…that aside from an ever-present undercurrent of disturbing thoughts related to the nature of reality, things are actually pretty good for me and my mood is up in spite of my being very aware that things could all go to hell any day now.
I guess I'm learning to live in the moment because I'm not sad or depressed and I can't say I'm anxious either… just mildly creeped out by my own wayward thoughts about things I can't prove. In all, I think this is probably the most stable state someone like me can hope to be in.


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July 5, 2005
Pirated Live 8 DVDs on eBay, industry protests
LONDON -- Internet auction site eBay said on Tuesday it had begun removing illegal DVD copies of the Live 8 poverty awareness pop concerts from its Web site, after the record industry complained.
Some of the pirate recordings on the site early on Tuesday were on sale within 24 hours of Saturday's concerts ending, and have been attracting bids of up to 16.99 pounds ($31) each.One of them boasts footage from huge concerts in London'sHyde Park and Philadelphia.
Ten concerts took place in all, from Tokyo in the east tonear Toronto in the west, and more than a million people turned up to see the greatest line-up of rock stars ever assembled.
While the concerts were free, British media said recordcompany EMI paid millions of pounds for the rights to releasethe official DVD of the event, which Bob Geldof organized to put pressure on world leaders to do more to beat poverty.
"There are too many people out there who believe music isfor stealing, regardless of the wishes of artists and thepeople who invest in them," said David Martin, director ofanti-piracy at the British Phonographic Industry (BPI).
"Sadly we are not at all surprised by this incident."
EBay said it had begun removing the listings.
"The unauthorized copies of Live 8 DVDs we have been told about have been taken down, because the sale of fake items is not permitted on eBay.co.uk," the site said in a statement.
EBay has already been labeled an "electronic pimp" byGeldof after free Live 8 tickets appeared on the site ahead of Saturday's concerts.
It suspended some of the accounts of users who placed hoaxbids for the tickets of up to 10 million pounds in order tosabotage the sales.
Geldof also organized the Live Aid charity gigs 20 years ago to raise money for Ethiopian famine victims, and broughtout a re-recording of the 1984 "Do They Know It's Christmas?"track to try to prevent bootleggers profiting from theoriginal.
The BPI urged eBay to toughen its safeguards againstpiracy, noting a dramatic rise in illegal sales.
In 2001, the BPI arranged for the removal of 2,315 illegalonline auctions, but in the first six months of this year thatnumber had risen to 13,280.


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A Hmong-American hip-hop/spoken word artist, a Syrian filmmaker and installation artist, and a Somali-born spoken-word artist shared their talents this month as part of the "Art Illuminating Human Rights" series of talks and performances. The twinning of the arts and human rights reflects the remarkable concentration of those communities in Minnesota. It also speaks to the fundamental nature of both — pursuing the truth of the human condition.
"It's inevitable that our sectors interconnect," said Fres Thao, the spoken-word artist and executive director of CHAT — the Center for Hmong Arts and Talent. "We support one another because as artists we are agents of change who have this platform, this medium, this power, this voice to tell the truth about what's happening in our culture at this time."
This particular platform came courtesy of a partnership between the Advocates for Human Rights and the Minneapolis Institute of Arts (now known as Mia).
Thao said the collaboration "allows art to be even more powerful and more effective and to go beyond art for art's sake and actually be intentional and meaningful and cause impact that will have a ripple effect."
The ripple could be a wave if extended to local arts organizations, which have a depth and breadth disproportional to the Twin Cities' population base. Many factors contribute to this cornucopia, including understanding that art is ultimately about humanity.
"Art is really a reflection of human experience and expression," explained Karleen Gardner, Mia's director of learning innovation. "It can really serve as a catalyst for bringing people together to engage in dialogue about really relevant issues in the world today."
And there's nothing more relevant today than human rights, especially with disparate conflicts causing desperate refugees in numbers unseen since World War II. Some of the response to the crises has come from a number of notable international institutions with local roots that support a global canopy.
"To me it's no surprise that people from Minnesota would want to have an impact globally," said Paul Walters, board vice president of the Minnesota International NGO Network (MINN), which lists 107 nongovernmental organizations, nonprofits, charitable, religious and other globally focused groups. Walters believes the sector is centralized here partly because, "We're politically engaged, we're globally engaged, so those two align, and there is a culture in Minnesota of addressing needs no matter where those needs are."
Human rights academics and advocates at the University of Minnesota have noticed — and helped create — this dynamic. The U will offer a new Master of Human Rights degree program starting next fall. It will be one of a handful of human rights programs in the U.S. that are not part of a law school, and though housed in the Humphrey School of Public Affairs, it will be supported jointly by the College of Liberal Arts.
The work of political leaders such as Harold Stassen, Hubert Humphrey, Walter Mondale, Don Fraser and others helped create the global focus that drives the organizations in MINN, said Barbara Frey, director of the U's Human Rights Program. Minnesota also became a welcoming new home for refugees over the years.
"It's hard to measure the chicken and the egg, but we became a center for refugees in part because of the excellent international services here, but then that very reputation ended up drawing a lot of human rights professionals to the Twin Cities," Frey said.
Working together can strengthen arts and human rights organizations in the region. "Great art has the power to illuminate the human condition," said Robin Phillips, executive director of the Advocates for Human Rights. "It also has the power to educate, inspire and soothe, and we know that a lot of people relate to human rights on an emotional level in addition to an educational level, and art makes that more accessible."
And there's a lot to access, said Essma Imady, who arrived in 2011, before Syria's version of the Arab Spring turned into what seems to be a permanent winter. Imady told the Mia attendees that the arts scene in Minnesota is "amazing."
Imady, a Masters of Fine Arts candidate at the Minneapolis College of Art and Design, spoke of a universal truth that can influence both art and human rights work. "It's sad, and yet beautiful, that we humans usually end up suffering in exactly the same way," she said.
John Rash is a Star Tribune editorial writer and columnist. The Rash Report can be heard at 8:20 a.m. Fridays on WCCO Radio, 830-AM. On Twitter: @rashreport.


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Music video by Paula DeAnda featuring Bow Wow performing Easy. (C) 2006 Arista Records, LLC, a unit of SONY BMG MUSIC ENTERTAINMENT.
"Easy" is the fourth single from Paula DeAnda's self titled album.
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He who joyfully marches in rank and file has already earned my contempt.
He has been given a large brain by mistake, since for him the spinal cord would suffice.(Albert Einstein)


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Another packed weekend on HCC. Here's what Jazz has brewed up for us this weekend.
The Director Kirk R. Thatcher
The Stars Ashanti, Queen Latifah, Jeffrey Tambor, David Alan Greir, Quentin Tarantino
The Plot Ashanti sparkles as Dorothy, an aspiring singer whose dreams of fame and fortune seem worlds away from coming true in the Kansas trailer park where she lives with her Aunt Em and Uncle Henry. But when a tornado transports her and Toto (Pepe the King Prawn) to the magical land of Oz, Dorothy meets the Wizard, who promises to make her a superstar -- if she and her wacky new friends, the Scarecrow (Kermit), the Tin Thing (Gonzo), and the Cowardly Lion (Fozzie), can defeat Oz's #1 diabolical diva, the Wicked Witch of the West (Miss Piggy)!
Fun Fact: When Kermit the Frog meets the Wizard of Oz he says "Hello Mr Oz. Are you any relation to Frank Oz?" This is an in-joke as Frank Oz was one of the real-life Muppeteers, best known for voicing the part of Miss Piggy.
#517 Richard Simmons - Original Airdate -- 12/20/2006
#102 "Zen or the Skill to Catch a Killer" - Original Airdate -- 4/12/1990
The Director Duwayne Dunham (Homeward Bound: the Incredible Journey, Little Giants)
The Stars Kyle McLachlan, Michael Ontkean, Madchen Amick, Dana Ashbrook, Richard Beymer, Lara Flynn Boyle, Sherilyn Fenn, Warren Frost, Peggy Lipton, James Marshall, Harry Goaz, Michael Horse, Everett McGill, Russ Tamblyn, Jack Nance, Ray Wise, Joan Chen, Piper Laurie, Eric Da Re, Sheryl Lee
The Plot Agent Cooper enjoys the comforts of the Great Northern Hotel; Bobby Briggs and Mike Nelson are released from jail; Doctor Hayward hands over the autopsy report on Laura Palmer; Cooper and Truman interview Josie Packard; Audrey Horne and Donna Hayward vow to solve Laura's murder; Sarah Palmer has a vision of a gray-haired man; Doctor Jacoby conceals important information.
Fun Fact 1: After the pilot movie, this was the first episode of the Twin Peaks TV series.
Fun Fact 2: In the scene where James Hurley is released from jail, watch carefully for the secret sign exchanged between Big Ed Hurley and Deputy Hawk. Its significance will be explained in later episodes.
Fun Fact 3: Some of the characters look somewhat different in this episode then they did in the pilot. Audrey and Leo have very different hair styles, even though only a few hours have passed since the end of the last episode. {This is due to the fact that many months seperated the filming of the pilot and the filming of Episode One.}
Fun Fact 4: ill Rogosheske, who plays Trudy, the waitress at the Great Northern Hotel, is married to writer/prdocuer Robert Engels. Trudy appears in many epiosdes, and also acted as the on set pianist for when the Icelanders or Leland break into song at the Great Northern.
The Director Alan Johnson (1983's To Be Or Not To Be)
The Stars Jason Patric (The Lost Boys, Speed 2: Cruise Control, Rush, Sleepers), Jami Gertz (The Lost Boys, Less Than Zero, Twister), Lukas Haas (Witness, The Lady In White, Rambling Rose), Richard Jordan (Logan's Run, The Secret of My Success, Gettysburg), Charles Durning, Peter DeLuise (21 Jump Street) , James LeGros (Drugstore Cowboy, Living In Oblivion, Guncrazy), Claude Brooks, Peter Kowanko (Amityville 3-D, Patty Hearst), Adrian Pasdar (Near Dark, Profit, Heroes), Sarah Douglas (Superman II, Conan the Destroyer, The Return of Swamp Thing)
The Plot In this excitement-on-wheels futuristic thriller, the desert wasteland of post-apocalyptic Earth is ruled by a sinister corporation known as the E-Protectorate. Beyond ruthless, the E-Protectorate hoards water and takes children from their families to train them to work for the corporation. But when a group of young rebels discovers an extraterrestrial sphere with healing powers, they set out to release the planet from the clutches of the oppressors. With its dazzling visual effects and mesmerizing score, Solarbabies is pure entertainment from start to finish.
Fun Fact: Produced by Brooksfilms, Mel Brooks' production company. Other non-comedic films produced by the company included David Lynch's The Elephant Man, Graeme Clifford's Frances and David Cronenberg's The Fly.
#306 "The Mirror" - Original Airdate -- 10/20/1961
The Director Don Medford (The Hunting Party, The Organization)
The Stars Peter Falk, Will Kuluva
The Plot After a poor but ambitious Central American farm worker overthrows his country's tyrannical leader, he believes he sees assassins everywhere. A look in the mirror reveals his most dangerous enemy
#102 "The Dark and Bloody Ground" - Original Airdate -- 9/20/1974
The Director Michael Schultz (Cooley High, Krush Groove, The Last Dragon)
The Stars James Garner, Noah Beery Jr.
The Plot Jim reluctantly agrees to work for Beth Davenport. His job is to help her prove that her client, Ann Calhoun, is innocent of the crime she is accused of...murdering her husband Kevin Calhoun. Jim's investigation takes him to the town of Parker, Arizona where the Calhouns are from. He digs up info someone doesn't want found out and he is almost killed trying to return to L.A. Socialites Clyde Russell and Elizabeth Gorman, who were throwing a party the Calhouns attended, are also investigated. Jim's investigation is making someone nervous as another attempt is made on his life
Fun Fact: This episode marks the first appearance of Gretchen Corbett as Beth Davenport.
3:51 Music Video - LINKIN PARK
"Faint" - Original Airdate 07/2003
The Director Mark Romanek (One Hour Photo)
#101 "The Reluctant Stowaway" - Original Airdate -- 9/15/1965
The Director Anton M. Leader (Children of the Damned)
The Stars June Lockheart, Angela Cartwright, Guy Williams, Marta Kristen, Mark Goddard, Bill Mumy, Jonathan Harris
The Plot In the year 1997, the Robinson family leave Earth to colonize Alpha Centauri. A spy from a foreign country, Dr. Smith, becomes trapped aboard while trying to sabotage the project. The ship is whisked off course and into a meteor storm due to Dr. Smith's extra weight upsetting the navigational computers. Smith desperately tries to save himself by reviving the crew resulting in the Robinson family becoming lost in space.
Fun Fact: In this first episode of the Lost In Space series, the voice of the president is provided by series producer Irwin Allen.
#108 "Benita's Double Trouble" - Original Airdate -- 10/31/1970
The Director Tony Charmoli
The Plot Peter Platter asks the Bugaloos to take over his show while he's at a DJ convention. Benita kidnaps Peter and makes him call the Bugaloos and say he wants her to do his show. In their rescue of Peter, the Bugaloos disguise themselves as Funky Rat, Woofer, Tweeter--and Benita!
Fun Fact: This was the second series to be produced by Sid Krofft and Marty Krofft after H.R. Pufnstuf. It also was the first one to be shot on videotape rather than film.
"A Plumbing We Will Go" - Original Release Date -- 9/19/1940
The Director Del Lord
The Stars Moe Howard, Larry Fine, Curly Howard
The Plot To escape the police, the stooges pose as plumbers and are hired to fix a leak in a fancy mansion. They wind up crossing the electrical system with the plumbing and generally ruin the place. One memorable scene has the lady of the house tuning into a television broadcast from Niagara Falls as a torrent of water pours from the set. To escape the wrath of the homeowners the stooges escape through a magicians trap door.
The Director Jeff Margolis (Richard Pryor Live In Concert)
The Plot This is an educational video, where Mr. T attempts to instill good values (honor thy mother, don't give in to peer pressure, etc..) to kids through Rapping and Breakdancing, among other things
1:00 Infomercial - KEEPERZ
1:15 Infomercial - FREEBIE FORCE
1:30 Infomercial - HEALTH VEST
2:00 Commercial - CHEERIOS ABOMINABLE SNOWMAN
2:10 Commercial - TEENAGE MUTANT NINJA TURTLES ACTION FIGURES
2:20 Commercial - BEN GAY
The Director Tobe Hooper (The Texas Chainsaw Massacre 1-2, Salem's Lot, Lifeforce)
The Stars JoBeth Williams (Stir Crazy, The Big Chill, Kramer vs. Kramer), Craig T. Nelson (Coach, The Killing Fields, The Incredibles), Heather O'Rourke, Beatrice Straight (Network), Dominique Dunne, Oliver Robins, James Karen, Dirk Blocker, Zelda Rubinstein
The Plot While living an an average family house in a pleasant neighborhood, the youngest daughter of the Freeling family, Carol Anne (Heather O'Rourke), seems to be connecting with the supernatural through a dead channel on the televison. It is not for long when the mysterious beings enter the house's walls. At first seeming like harmless ghosts, they play tricks and amuse the family, but they take a nasty turn- they horrify the family to death with angry trees and murderous dolls, and finally abduct Carol Anne into her bedroom closet, which seems like the entrance to the other side.
Fun Fact 1: This film is still surrounded by controversy. Although listed primarily as executive producer, Steven Speilberg is thought by some to have directed the majority of the film with Tobe Hooper having little input into the final product. There are still conflicting stories on both sides of the issue to this day.
Fun Fact 2: The hands which pull the flesh off the investigator's face in the bathroom mirror are Steven Speilberg's.
Fun Fact 3: There is legend of a curse following this trilogy of films because of the tragedies that befell its supporting cast. Dominique Dunne was killed by her boyfriend the year this was released. Julian Beck and Will Sampson of Poltergeist II died in 1985 and 1987. Heather O'Rourke died on the set of Poltergeist III in 1988. Dunne and O'Rourke are buried in the same cemetery. Dunne's killer was convicted of manslaughter and served less than four years in prison, just in case you wanted to add some anger to the sadness.
#104 "Menace from Space" - Original Airdate -- 9/18/1986
The Plot Chuck Norris and his comrades head off to a space shuttle launch site in order to warn a general about The Claw's plans, though he is too late and The Claw manages to get away with the shuttle. To make matters worse, the space shuttle was carrying a fusion cannon that has the power to do unspeakable damage to any city in the world, so Chuck Norris decides to use an old space shuttle in order to go into space and stop Super Ninja before any damage is dealt.
Fun Fact: Chuck Norris would give moral lessons witch each episode of this series. In this episode, he stresses having the strength to do something even if its something you don't want to do. He tells kids to do their homework instead of watching TV all day.
#104 "Pros and Cons" - Original Airdate -- 2/18/1983
The Director Ron Satloff
The Stars George Preppard, Dirk Benedict, Dwight Schultz, Mr. T, Melinda Culea, William Lucking, Paul Koslo, Clifton James, William Smith
The Plot B.A.'s friend is held in a prison where the director arranges fight-to-the-death-boxing matches whose winner gets released, only to be hunted down and killed. So the A-Team lets themself be arrested. Inside B.A. soon has to prepare to fight.
Fun Fact: Face pretends to be the author of a novel Dr. Dwight Pepper. The photo on the back of the book - supposedly the proper Dr. Dwight Pepper - is actually a photo of Stephen J. Cannell, the producer of the series.
Thursday, October 16, 2008
THIS WEEKEND ON HCC! - 10/17-10/18... Muppets, Rollerskates, Mr. T!
Posted by Scott W. Davis at 10:47 PM
Labels: blog, capper, capping, caption this, darren mcgavin, hipsoda, invisible hoagie, mst3k, mystery science theatre 3000, satire, schedule, sci-fi channel, shablorp, tv guide, update
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After years of suffering with ill-fitting replacement parts, I have a Terminal Block that fits!
It presses firmly onto the distirbutor body, has an ultra-flexible wire with 350 strands of supple copper wire, a silicon jacket (think premium spark plug wire jackets), and brass/steel terminals for great conductivity and durability. . Replace that shabby old questionable terminal block now!
This fits all 22D, 23D, and 25D distirbutors. Older DM2 distributor with a "fork" on the breaker plate can be modified to use this terminal block as well!


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- Register
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Gene editing technique CRISPR-Cas9 has heralded in what has been called the "Golden Age of Genetic Engineering." Since the technique exploded onto the mainstream research scene two years ago, there have been countless advances, discoveries and refinements to both CRISPR and related methods. What's especially impressive about CRISPR is how it's transforming the work of so many scientists in so many different fields. At this early juncture, the applications seem endless. As research into CRISPR increases, so too will our understanding of the crucial biological mechanisms that drive human bodies on an everyday basis.


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Set of Four Mastercraft Bronze Base Dining Chairs
Set of Four Mastercraft Bronze Base Dining Chairs
A luxurious and modern set of four dining chairs by Mastercraft with bronze finish base and newly upholstered in a durable, textured white fabric and single welt piping. Remarkable craftsmanship.
USA, 1970's.
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39.5"H x 29.5"D x 18.25"W
18.5" seat height
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View this item in our Chicago Showroom. South Loop Loft is located in Chicago's West Town neighborhood, 308 N Leavitt, Chicago, Illinois 60612. We are open on Saturdays from 11-5 and By Appointment. Please call or email us to schedule an appointment at email@example.com or 262-490-4215.
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Reactive Oxygen Species Regulate Heat-Shock Protein 70 via the JAK/STAT Pathway
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Abstract—Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) activate intracellular signal transduction pathways implicated in the pathogenesis of cardiovascular disease. H2O2 is a mitogen for rat vascular smooth muscle cells (VSMCs), and protein tyrosine phosphorylation is a critical event in VSMC mitogenesis. Therefore, we investigated whether the mitogenic effects of H2O2, such as stimulation of extracellular signal–regulated kinase (ERK)2, are mediated via activation of cytoplasmic Janus tyrosine kinases (JAKs). JAK2 was activated rapidly in VSMCs treated with H2O2, and signal transducers and activators of transcription (STAT) STAT1 and STAT3 were tyrosine-phosphorylated and translocated to the nucleus in a JAK2-dependent manner. Inhibition of JAK2 activity with AG-490 partially inhibited H2O2-induced ERK2 activity, suggesting that JAK2 is upstream of the Ras/Raf/mitogen-activated protein kinase–ERK/ERK mitogenic pathway. Because heat-shock proteins (HSPs) can protect cells from ROS, we investigated the effect of H2O2 on HSP expression. H2O2 stimulated HSP70 expression in a time-dependent manner, and AG-490 abolished H2O2-induced HSP70 expression. H2O2 activated the HSP70 promoter via enhanced binding of STATs to cognate binding sites in the promoter. Regulation of chaperones such as HSP70 via activation of the JAK/STAT pathway suggests that in addition to its growth-promoting effects, this pathway may help VSMCs adapt to oxidative stress.
This work was supported by National Institutes of Health grants HL57352 and HL61656.
- Received October 12, 2000.
- Accepted December 15, 2000.
Accumulating evidence supports a critical role for oxidative stress in the pathogenesis of atherosclerosis, cancer, and other human diseases.1 High levels of reactive oxygen species (ROS) damage DNA and inactivate proteins,2 resulting in chronic cellular dysfunction. Many cell types have also harnessed ROS, albeit in lower concentrations, as intracellular signaling molecules to mediate growth factor and cytokine responses.3 4 Modulation of growth responses by ROS has been demonstrated in a number of cell types, including vascular smooth muscle cells (VSMCs).5 6 Stimulation of VSMC proliferation by ROS is thought to be a critical step in atherosclerotic lesion formation.7
Tyrosine phosphorylation of cellular proteins and the consequent induction of transcription of early-response genes are key determinants of cell growth and differentiation in response to mitogenic signaling.8 VSMC mitogens such as platelet-derived growth factor and epidermal growth factor activate receptor protein tyrosine kinases on binding, which stimulate intracellular signaling pathways that result in mitogen-activated protein kinase activation.9 10 Other mitogens, such as thrombin and angiotensin II, activate G protein–coupled receptors that do not possess intrinsic tyrosine kinase activity but require tyrosine phosphorylation events to induce mitogenesis.11 12 13
The necessary role for protein tyrosine phosphorylation in mitogenesis elicited by thrombin and ROS indicates that these mitogens may utilize cytoplasmic protein tyrosine kinases in their signaling cascade. Forming 1 such group of tyrosine kinases are Janus kinases (JAKs), which along with their substrates, signal transducers and activators of transcription (STATs), have hitherto been characterized as essential mediators of cytokine and polypeptide hormone-induced signaling.8 14 Members of the JAK/STAT pathway mediate at least some biological effects of angiotensin II,15 platelet-derived growth factor-BB,15 16 and endothelial growth factor.17 Activation of the JAK/STAT pathway has also been observed in response to generation of intracellular ROS18 and exogenous hydrogen peroxide (H2O2).19 On phosphorylation by JAKs of tyrosine residues, activated STAT dimers translocate to the nucleus to transactivate target gene expression.20
The 70- and 90-kDa heat-shock proteins (HSPs) are among the subset of stress-responsive proteins known to be transactivated by STATs.21 22 Elevated levels of HSPs are rapidly synthesized within the cell in response to environmental stresses.23 Under physiological conditions, constitutively expressed HSPs function as molecular chaperones, whereas under stress conditions, HSPs prevent protein aggregation.24 In addition, HSPs may directly regulate specific stress-responsive signaling pathways and may antagonize signaling cascades that result in apoptosis.25
Because ROS do not directly activate receptor protein tyrosine kinases to exert their mitogenic effects on VSMCs, we investigated the effect of H2O2 on the activation of cytoplasmic tyrosine kinases, JAKs, and their substrates, STATs. We show here that H2O2 causes activation of JAK2 and subsequent tyrosine phosphorylation and nuclear translocation of STAT1 and STAT3. Our results also indicate that JAK2 partially regulates extracellular signal–regulated kinase (ERK)2 activity and thus, contributes to VSMC proliferation. Furthermore, we demonstrate that the JAK/STAT pathway mediates H2O2-induced HSP70 expression, which may help cells adapt to oxidative stress.
Cell Culture and Reagents
VSMCs were isolated from the thoracic aortas of 200- to 250-g male Sprague-Dawley rats. Cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% (vol/vol) calf serum as described previously.12 VSMCs were growth-arrested by incubation in Dulbecco's modified Eagle's medium containing 0.1% calf serum for 72 hours. Antibodies used were as follows: anti-phosphotyrosine (4G10), anti-JAK1, anti-JAK2, anti-TYK2, anti-STAT1, anti-STAT3, and anti-ERK2 (UBI and Santa Cruz Biotechnology); anti-phosphospecific JAK2 (Biosource International); anti-phosphospecific and anti-nonphosphospecific ERK1/2 (New England Biolabs); and anti-HSP70 (Affinity BioReagents). AG-490 was obtained from Calbiochem.
Immunoprecipitation, ERK2 Activity Assay, and Western Blotting
Growth-arrested VSMCs were treated with 200 μmol/L H2O2 in the presence or absence of inhibitors for the specified time periods at 37°C. Cell lysates were prepared, and immunoprecipitation, ERK2 activity assay, and Western blotting were performed as described previously.26
Electrophoretic Mobility Shift Assay
Nuclear extracts were prepared from growth-arrested VSMCs that were either left untreated or treated with 200 μmol/L H2O2.27 DNA binding was performed by incubating 5 μg of nuclear protein with 100 000 counts per minute of 32P-labeled, double-stranded HSP70 oligonucleotide (−122GATCCGGCGAAACCCCTGGAATATTCCCCGACCT−90) for 20 minutes at room temperature. Canonical double-stranded oligonucleotides for SP1 (5′-ATTCGATCGG-GGCGGGGCGAGC-3′) and STAT3 (5′-TGATTACGGGAAATG-3′) and a high-affinity, double-stranded STAT1 binding sequence SIEm67 (5′-GATCTGATTACGGGAAATG-3′)28 were used in competition studies. For identifying the STAT1-specific band, samples were incubated with STAT1 or STAT3 antibody for 30 minutes before the DNA binding reaction was performed. Protein-DNA complexes were resolved on a 4% polyacrylamide gel.
Transient Transfection and CAT Assay
The HSP70 chloramphenicol acetyltransferase (CAT) reporter constructs LSN (−188 to +1) and LSNP (−100 to +1) were kindly provided by Richard Morimoto (Northwestern University, Evanston, Ill). VSMCs at 70% to 80% confluence were transfected with 10 μg of reporter plasmid DNA by using the calcium precipitate method.26 Cells were cotransfected with β-galactosidase expression vector to normalize for transfection efficiency and were quiesced for 16 hours after transfection. After quiescence was maintained for 36 hours, cells were either left untreated or treated with 200 μmol/L H2O2 for 6 hours. In experiments with AG-490, cells were treated with the inhibitor for 16 hours before H2O2 treatment. Cell lysates were prepared,26 and CAT activity was measured by the method of Gorman et al.29 The β-galactosidase assay was performed by following the manufacturer's protocol (Promega).
Statistical Analysis
Differences were analyzed with 1-way ANOVA, and post hoc analysis was performed with the Student-Newman-Keuls method. Values of P<0.05 were considered statistically significant.
H2O2 Causes Activation of Kinases JAK2 and TYK2 in Rat VSMCs
To test the hypothesis that JAK/STAT pathway activation contributes to the mitogenic effects of ROS on VSMCs, we treated growth-arrested VSMCs with 200 μmol/L H2O2, a physiological concentration of this representative ROS that elicits VSMC proliferation.6 H2O2 caused rapid tyrosine phosphorylation of JAK2, which was sustained for 60 minutes (Figure 1A⇓), but it had no effect on JAK1 phosphorylation (data not shown). JAK2 phosphorylation was biphasic, with peaks at 5 minutes (9.10±2.97-fold increase) and 30 minutes (5.0±1.52-fold increase; Figures 1A⇓, 1B⇓ [top], and 1C). A similar biphasic stimulation of various kinases by ROS has been reported, and the second peak has been attributed to the secretion of autocrine factors.30 Tyrosine phosphorylation of JAK2 by H2O2 was concentration dependent, and maximum phosphorylation was observed with 200 μmol/L H2O2 at 5 minutes (please see http://www.atvb.ahajournals.org). In contrast to JAK2, peak TYK2 tyrosine phosphorylation was observed after a 15-minute exposure to H2O2, and TYK2 phosphorylation (3.86±0.64-fold increase) was not as pronounced as that of JAK2 (please see http://atvb.ahajournals.org). H2O2 altered tyrosine phosphor- ylation without affecting steady-state protein levels of JAK2 (Figure 1B⇓, bottom) and TYK2 (please see http://atvb.ahajournals.org).
H2O2 Induces Tyrosine Phosphorylation and Nuclear Translocation of STAT1 and STAT3 in Rat VSMCs
To determine whether activation of JAK2 and TYK2 by H2O2 leads to activation of STAT proteins, H2O2-treated VSMC lysates were immunoprecipitated with an anti-phosphotyrosine antibody, and immunoprecipitates were immunoblotted with antibodies against STAT1α/β or STAT3. Constitutive phosphorylation was lower for STAT1α than for STAT1β in untreated VSMCs (Figure 2A⇓), but STAT1α phosphorylation was greater than that for STAT1β after 10 minutes of H2O2 treatment. Tyrosine-phosphorylated STAT1α/β were not detectable 15 and 30 minutes after H2O2 treatment, and phosphorylated STAT1α/β levels at 60 minutes were much lower than in untreated cells. In broad terms, the biphasic STAT1 phosphorylation corresponded to the biphasic JAK2 phosphorylation, suggesting that the former is dependent on JAK2 activation. STAT1 was rapidly translocated to the nucleus within 5 minutes after H2O2 treatment (Figure 2B⇓, top), whereas no detectable change was observed in cytosolic STAT1 protein levels (Figure 2B⇓, bottom). Peak nuclear translocation of STAT1 was observed at 10 minutes (4.03±0.32-fold increase), and in contrast to the tyrosine phosphorylation in immunoprecipitation studies (Figure 2A⇓), was sustained for 60 minutes (Figure 2C⇓). The reason for this discrepancy could be that nuclear fractions may contain some dephosphorylated STAT1. Constitutive tyrosine phosphorylation of STAT3 was observed in growth-arrested VSMCs, and phosphorylation increased within 5 minutes of H2O2 treatment and was sustained for 60 minutes (Figure 3A⇓). As with STAT1, rapid nuclear translocation of STAT3 was observed in H2O2-treated VSMCs (Figure 3B⇓, top), with no detectable change in protein levels in the cytosolic fractions (Figure 3B⇓, bottom). Peak nuclear STAT3 protein levels were observed at 15 minutes (2.90±0.49-fold increase; Figure 3C⇓) and were sustained for 60 minutes. These experiments demonstrate the phosphorylation and nuclear translocation of STATs in H2O2-stimulated VSMCs.
JAK2 Contributes to ERK2 Activation in Response to H2O2 in VSMCs
Because ERK1/2 activation is associated with H2O2-induced mitogenesis,31 32 we investigated the relationship between JAK2 stimulation by H2O2 and ERK2 activity. Pretreatment of VSMCs with 50 μmol/L AG-490, a specific JAK2 inhibitor, inhibited H2O2-induced JAK2 tyrosine phosphorylation and the consequent phosphorylation of STAT1 and STAT3, whereas it had no effect on c-Src, a non-JAK cytosolic tyrosine kinase (data not shown). Peak ERK2 activity, as measured by an immunocomplex kinase assay, was observed 15 minutes after treatment with H2O2 (Figure 4⇓). This increase in ERK2 activation by H2O2 is consistent with 1 previous report32 but is contradictory to another33 ; the reasons for this discrepancy are not clear. H2O2-induced ERK2 activation was blocked partially by AG-490 (4.5±1.4-fold increase with H2O2 at 15 minutes vs 2.7±0.7 and 1.9±0.4-fold for H2O2 in the presence of 10 and 50 μmol/L AG490, respectively), suggesting that JAK2 activation is necessary for H2O2-induced ERK2 activation. Partial inhibition of ERK2 activity by a JAK2 antagonist indicates that, along with JAK2-mediated stimulation, there may be an alternative pathway for ERK1/2 activation.
H2O2 Induces HSP70 Expression Through Activation of the JAK/STAT Pathway
Accumulation of RNA for HSPs has been reported during conditions known to produce ROS.34 The 5′-flanking sequences of the HSP70 and HSP90β genes contain functional binding sites for STATs.21 Hence, we investigated whether H2O2 causes accumulation of these proteins in rat VSMCs. H2O2 stimulated expression of both HSP70 (Figure 5⇓) and HSP90 (data not shown). Because of more pronounced upregulation, we further investigated the expression of HSP70. HSP70 protein levels increased in VSMCs in a time-dependent manner, with a 5.90±0.97-fold increase at 24 hours after 200 μmol/L H2O2 treatment (Figures 5A⇓ and 5B⇓). Pretreatment of VSMCs with 50 μmol/L AG-490 abolished the increase in HSP70 protein levels induced by H2O2 at 24 hours (Figures 5C⇓ and 5D⇓; 5.70±0.85 vs 1.40±0.50, P<0.05).
To investigate whether HSP70 expression in VSMCs exposed to H2O2 is mediated by the binding of STATs to their responsive element (−122 to −90 bp of the HSP70 promoter), we measured the ability of nuclear proteins from H2O2-treated cells to bind an HSP70-STAT oligonucleotide (please see http://www.atvb.ahajournals.org). Nuclear extracts from H2O2-treated VSMCs produced 2 shifted bands that were competed with an excess of unlabeled specific oligonucleotide but not with a nonspecific sequence. The faster migrating band was competed with an unlabeled, high-affinity STAT1 binding site (SIEm67 oligonucleotide) from the c-fos promoter, but not with an unlabeled STAT3 consensus sequence, and was abolished by preincubation of the complexes with an anti-STAT1 antibody (but not by an anti-STAT3 antibody), demonstrating the presence of STAT1 protein in this complex. The slower migrating band was partially competed by STAT1 and STAT3 oligonucleotides and partially abolished by anti-STAT1 and anti-STAT3 antibodies, suggesting that it contains a STAT1/STAT3 heterodimer.
H2O2 activates the HSP70 promoter in a JAK2-dependent manner. To investigate whether H2O2-induced HSP70 expression was mediated via a direct effect on its promoter, VSMCs were transfected with HSP70 promoter-reporter constructs either containing (LSN, −188 to +1) or lacking (LSNP, −100 to +1) a functional STAT binding site. The reporter construct LSN was activated 2.5-fold by 200 μmol/L H2O2, whereas transactivation in response to H2O2 was lost by deletion of the STAT binding site (Figure 6A⇓). AG-490 inhibited the H2O2-induced increase in HSP70 promoter activity, suggesting that STATs require JAK2 activation for activity (Figure 6B⇓). Taken together with the results shown in Figure 5⇑, these findings indicate that H2O2 activates the JAK/STAT pathway in VSMCs, which has functional consequences on the expression of physiologically relevant proteins such as HSP70.
We have shown that H2O2 stimulates specific members of the JAK/STAT pathway, an effect that participates in stress-responsive gene expression. Rapid activation of different JAKs and STATs in response to H2O2 has recently been reported in fibroblasts and lymphocytes.18 19 35 In this report, we link JAK2 activation by H2O2 with STAT phosphorylation and specific transactivation of a STAT-responsive gene promoter.
H2O2 stimulates ERK2 activity in VSMCs in a time-dependent manner, with peak activity occurring at 15 to 30 minutes. Inhibition of JAK2 activity by AG-490 pretreatment partially inhibits H2O2-stimulated ERK2 activity (Figure 4⇑). In contrast, a temporal shift in ERK1/2 activation was observed in fibroblasts treated with H2O2 and AG-490.35 The formation of a Ras/JAK2/Raf-1 complex after JAK2 phosphorylation, resulting in Ras and Raf-1 activation, was initially thought to be a cytokine-induced response.36 Emerging evidence indicates that JAK2 activation is also required for angiotensin II– and platelet-derived growth factor–induced JAK2/Raf-1 complex formation, Raf-1 tyrosine phosphorylation, and ERK1/2 activation.15 Recently it was reported that H2O2-mediated Ras and ERK1/2 activations are partially dependent on JAK2 activity. Based on those findings, it is proposed that JAK2 is upstream of Ras in the Ras/Raf/mitogen-activated protein kinase–ERK/ERK pathway and thus, regulates activation of early growth–response genes and cell proliferation, although a parallel H2O2-stimulated pathway independent of JAK2 must also exist to activate ERK1/2.35 Cross-talk between the JAK/STAT and ERK pathways suggests that inhibition of JAK2 activity not only blocks direct tyrosine phosphorylation but can also indirectly inhibit serine phosphorylation of STATs by ERK1/2. Maximal transactivation by STATs requires serine phosphorylation by mitogen-activated protein kinase besides tyrosine phosphorylation.37 On the contrary, ERK1/2 activation inhibits interleukin-6–induced JAK/STAT signaling.38 Further experiments will define the role of ERK1/2 in the activation of STATs by H2O2.
Increased HSP70 protein synthesis is seen in cardiovascular tissues exposed to various stressors39 and in VSMCs surrounding the necrotic zones of atherosclerotic plaques.40 Enhanced HSP70 protein levels protect against lethal heat stress and ischemia.41 ROS such as H2O2 have pleiomorphic effects on cells, eliciting apoptosis in some cell types42 while stimulating early growth–response gene expression and proliferation in others.5 The cell types that exhibit proliferative responses likely have adaptive mechanisms to overcome the adverse effects of oxidants. Because HSPs modulate the stress response, we hypothesized that they might be induced in VSMCs exposed to H2O2, especially because HSP promoters contain functional STAT binding sites.21 Our results demonstrate a time-dependent increase in HSP70 protein levels in VSMCs treated with H2O2, which is blocked by pretreatment with AG-490, indicating that this protein is indeed regulated via activation of the JAK/STAT pathway. HSP70 has been reported to promote cell proliferation.43 Oxidative stress–induced HSP90α and cyclophilin may promote VSMC growth.37 These observations suggest that regulation of HSP70 could be 1 of the mechanisms by which the H2O2-induced JAK/STAT pathway promotes VSMC growth. Further studies will determine the growth-promoting versus protective effects of HSP70 on H2O2-induced VSMC growth.
Our results also indicate that the HSP70 promoter construct containing a functional STAT-binding site is activated by H2O2, which is blocked by pretreatment with AG-490. JAK2-mediated phosphorylation of STATs is therefore required for HSP70 promoter activation by H2O2. STAT1 is the major protein binding the STAT-responsive element in VSMCs, although it is possible that STAT3 also participates, as STAT3 is rapidly phosphorylated after H2O2 treatment and an anti-STAT3 antibody partially competes for H2O2-induced binding activity. In addition to STAT binding sites, the HSP70 promoter (LSN, −188 to +1) also has overlapping binding sites for another transcription factor, heat-shock factor-1.21 Although we do not specifically address this point, it is possible that heat-shock factor-1 may also be involved in H2O2-mediated HSP70 expression in VSMCs. Indeed, significant heat-shock factor-1 activation has been observed in heart tissue perfused with H2O2.44 Stephanou et al21 have shown that overexpression of STAT1 and heat-shock factor-1 has additive effects on HSP70 promoter activity in HepG2 cells and suggest that protein-protein interactions may play a role in transcriptional activity. The demonstration of enhanced HSP70 expression in VSMCs treated with H2O2 via the JAK/STAT pathway suggests that, in addition to its growth-promoting effects, this pathway may play a key role in the adaptive response to oxidative stress.
We are thankful to Joann Aaron and Chris Horaist for editorial assistance.
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- ↵Shacter E, Beecham EJ, Covey JM, Kohn KW, Potter M. Activated neutrophils induce prolonged DNA damage in neighboring cells. Carcinogenesis. 1998;9:2297–2304.
- ↵Sundaresan M, Yu Z-X, Ferrans VJ, Irani K, Finkel T. Requirement for generation of H2O2 for platelet derived growth factor signal transduction. Science. 1995;270:296–299.
- ↵Thannickal VJ, Hassoun PM, White AC, Fanburg BL. Enhanced rate of H2O2 release from bovine pulmonary artery endothelial cells induced by TGF-β1. Am J Physiol. 1993;265:L622–L626.
- ↵Duque I, Puyol MR, Ruiz P, Gonzalez I-R, Marques MLD, Puyol DR. Calcium channel blockers inhibit hydrogen-peroxide induced proliferation of cultured rat mesangial cells. J Pharmacol Exp Ther. 1993;267:612–616.
- ↵Rao GN, Berk BC. Active oxygen species stimulate vascular smooth muscle cell growth and proto-oncogene expression. Circ Res. 1992;70:593–599.
- ↵Griendling KK, Alexander RW. Oxidative stress and cardiovascular disease. Circulation. 1997;96:3264–3265.
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- ↵Arvidsson A-N, Rupp E, Nanberg E, Downward J, Ronnstrand L, Wenstrom S, Schlessinger J, Heldin C-H, Claesson-Welsh L. Tyr-716 in the platelet-derived growth factor β-receptor kinase insert is involved in Grb-2 binding and Ras activation. Mol Cell Biol. 1994;14:6715–6726.
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- ↵Molloy CJ, Taylor DS, Weber H. Angiotensin II stimulation of rapid protein tyrosine phosphorylation and protein kinase activation in rat aortic smooth muscle cells. J Biol Chem. 1993;268:7338–7345.
- ↵Rao GN, Delafontaine P, Runge MS. Thrombin stimulates phosphorylation of insulin like growth factor-1 receptor, insulin receptor substrate-1, and phospholipase C-ψ1 in rat aortic smooth muscle cells. J Biol Chem. 1995;270:27871–27875.
- ↵Weiss RH, Nuccitelli R. Inhibition of tyrosine phosphorylation prevents thrombin-induced mitogenesis, but not intracellular calcium release, in vascular smooth muscle cells. J Biol Chem. 1992;267:5608–5613.
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- ↵Marrero MB, Schieffer B, Li B, Sun J, Harp JB, Ling BN. Role of Janus kinase/signal transducer and activator of transcription and mitogen-activated protein kinase cascades in angiotensin II- and platelet-derived growth factor-induced vascular smooth muscle cell proliferation. J Biol Chem. 1997;272:24684–24690.
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- ↵Zhong Z, Wen Z, Darnell JE Jr. STAT3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Science. 1994;264:95–98.
- ↵Simon AR, Rai U, Fanburg BL, Cochran BH. Activation of the JAK-STAT pathway by reactive oxygen species. Am J Physiol. 1998;275:C1640–C1652.
- ↵Carballo M, Conde M, Bekay RE, Nieto JM, Camacho MJ, Monteseirin J, Conde J, Bedoya FJ, Sobrino F. Oxidative stress triggers STAT3 tyrosine phosphorylation and nuclear translocation in human lymphocytes. J Biol Chem. 1999;274:17580–17586.
- ↵Darnell JE Jr, Kerr IM, Stark GR. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science. 1994;264:1415–1421.
- ↵Stephanou A, Isenberg DA, Nakajima K, Latchman DS. Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and HSP-90β gene promoters. J Biol Chem. 1999;274:1723–1728.
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- ↵Benjamin IJ, McMillan DR. Stress (heat shock) proteins: molecular chaperones in cardiovascular biology and disease. Circ Res. 1998;83:117–132.
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- ↵Rao GN, Katki KA, Madamanchi NR, Wu Y, Birrer MJ. JunB forms the majority of the AP-1 complex and is a target for redox regulation by receptor tyrosine kinase and G protein-coupled receptor agonists in smooth muscle cells. J Biol Chem. 1999;274:6003–6010.
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- ↵Gorman CM, Moffat LF, Howard BH. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982;2:1044–1051.
- ↵Liao D-F, Jin Z-G, Baas AS, Daum G, Gygi SP, Aebersold R, Berk BC. Purification and identification of secreted oxidative stress-induced factors from vascular smooth muscle cells. J Biol Chem. 2000;275:189–196.
- ↵Pages G, Lenormand P, L'Allemain G, Chambard J, Meloche S, Pouyssegur J. Mitogen-activated protein kinases p42 mapk and p44 mapk are required for fibroblast proliferation. Proc Natl Acad Sci U S A. 1993;90:8319–8323.
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- ↵Baas AS, Berk BC. Differential activation of mitogen-activated protein kinases by H2O2 and O2− in vascular smooth muscle cells. Circ Res. 1995;77:29–36.
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- ↵Abe J, Berk BC. Fyn and Jak2 mediate ras activation by reactive oxygen species. J Biol Chem. 1999;274:21003–21010.
- ↵Winston LA, Hunter T. Jak2, Ras, and Raf are required for activation of extracellular signal regulated kinase/mitogen-activated protein kinase by growth hormone. J Biol Chem. 1995;270:30837–30840.
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- ↵Sengupta TK, Talbot ES, Scherle PA, Ivashkiv LB. Rapid inhibition of interleukin-6 signaling and STAT3 activation mediated by mitogen-activated protein kinases. Proc Natl Acad Sci U S A. 1998;95:11107–11112.
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- ↵Johnson AD, Berberian PA, Tytell M, Bond MG. Differential distribution of 70-kD heat shock protein in atherosclerosis: its potential role in arterial SMC survival. Arterioscler Thromb Vasc Biol. 1995;15:27–36.
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- ↵Nishizawa J, Nakai A, Matsuda K, Komeda M, Ban T, Nagata K. Reactive oxygen species play an important role in the activation of heat shock factor 1 in ischemic-reperfused heart. Circulation. 1999;99:934–941.
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- Reactive Oxygen Species Regulate Heat-Shock Protein 70 via the JAK/STAT PathwayNageswara R. Madamanchi, Suzhen Li, Cam Patterson and Marschall S. RungeArteriosclerosis, Thrombosis, and Vascular Biology. 2001;21:321-326, originally published March 1, 2001https://doi.org/10.1161/01.ATV.21.3.321
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Founded in 1981, Valley Eye Associates was established on one basic principle: the commitment to eye care excellence. Since that time, our clinic has grown to become one of Wisconsin's premier eye care institutes. Whether you are looking for a routine eye exam to help maintain good ocular health, glasses or contacts to help you see clearly, or surgical options to give you the freedom of spectacle-free vision, Valley Eye Associates is here for you.
We are proud to have 5 locations with 11 optometrists and 5 ophthalmologists who can offer you the following services:


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World map Walnut
These pictures are taken in the process of installing and treating the MapaWall wooden world map with oil. On one of the pictures you can see how our client uses the 8.2 foot long (1ste version) template in order to pick a spot for the world map to hang. The current/new version of the template illustrates all continents and islands, and makes it able to mark all points for the attachments. This way you wont have to measure anything anymore to get everything in the exact right position. The other photo's illustrate what the MapaWall Walnut with country borders looks like when it's just oiled. In some pictures the oil still needs to set. So in some pictures oil reflexes on the flashlight of the camera.
Place: Heerhugowaard | the Netherlands
Date: February 2016
Type of veneer: Walnut
Country borders: yes
visit shop


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Reel Shorts 2014
- 10 minutes
- South Africa
- 2013
Simoné Botha was born completely deaf in both ears and was the youngest recipient of cochlear implant surgery in Africa at the age of 22 months. Against enormous odds, she has succeeded in becoming a professional ballet dancer.
- Danielle Sher


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We've been waiting for the first video featuring Lemuria to -- pardon the pun -- surface, and now we're happy to report that it's here! On top of the visually stunning fly through of Champions Online's mid-level underwater zone, IGN also has an interview with Bill Roper that covers a good amount of ground concerning the first questions that popped into our head when Lemuria was originally revealed.
Namely, Roper answers our burning questions on fire-based powers underwater and breathing mechanics. A pill developed by the in-game faction called UNTIL will allow heroes to breath with ease and for the sake of fun. Who wants to resurface every five or ten minutes, anyhow?
And as for the water-unfriendly powers, Cryptic is simply taking the "this is a comic book-based world" approach and not messing with anything. However, travel powers apparently aren't going to work underwater, which makes sense. We only hope there's some way to compensate people who spend some time underwater. Giving out a water propeller backpack after completing the first few quest chains couldn't hurt.


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"You know Ted, you have been gifted above any man that I know," Rafael Cruz, our hyperbolic senator's dad, recently said he told his son starting at age four. "And God has destined you for greatness."
That was in an interview with the Christian Broadcasting Network, but it's hardly the first time Rafael Cruz has made himself heard. The Cruz men are beginning travel regularly to right-wing events like "Free The People" together, where Rafael excoriates President Obama and progressive policies. In early August, the Cruz show will head to 2016-essential Iowa for the "Family Leadership Summit".
Rafael Cruz is actually well-practiced in conservative politics. When Cruz was a child, he sat on the Religious Roundtable, a largely-Christian but partially-Jewish group working to get Reagan elected. "[W]hen my son Ted was eight years old, all we talked about around the dinner table was politics because I was so involved with the Reagan campaign," Cruz said in the same CBN interview. He is clearly thrilled to share part of his son's new spotlight, and he obviously had a heavy influence on Ted's thinking.
But does this paternal road show actually help Cruz? Read more below the jump.While deeming one's son anointed may play well with the evangelical crowd, it's safe to say it seems creepy to many others. It's the type of self-aggrandizement Americans recoiled at when President Bush said he talks to god. It indicates an irrational degree of pious self-assurance that has proven far more dangerous in America than helpful.
But the senior Cruz is extreme in secular ways too. He recently said President Obama is like Fidel Castro for promising to use executive authority on available issues that Congress fails to act upon. "Not much different than that old bearded friends that I left behind in Cuba; governing by decree, by executive order just like a dictator, like Fidel Castro," Cruz said. Again – this plays well at tea party events, but it's an ugly and unappealing thing to most Americans. As always with the Cruz men, their vitriolic views are cloaked in airs of piety and concern. But that doesn't adequately hide how vile such rhetoric is.
Rafael makes it impossible to forget that he is from Cuba and spent a lot of his life in Canada before coming to the United States. Ted Cruz opposes all pathways to citizenship for undocumented immigrants, a paradox because he has such recent family immigration history. It seems unlikely that trotting out his ultra-conservative, ultra-religious father will make that position seem more soft to a country in which only 13 percent of people oppose a pathway to citizenship. Rafael may inadvertently highlight the depravity of Ted's views.


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Yep, that's right...*sings* Happy Birthday to me (^_^) It's truly amazing to think about how many of my now 26 years have been devoted to anime et al. I still remember when I first set eyes on Dragon Ball, Kimagure Orange Road, or even Totoro during my single-digit years. Just imagine how much its branched off from there.
So anyways, I just wanted to take the time and thank everyone for their wonderful birthday wishes (my FaceBook wall is full of them), lunches, dinners, and the presents I will truly treasure -- including a the beautiful DMC4 Dante figure by Kotobukiya, an always handy gift card to GameStop, of course the Sony Cyber-shot DSCH50 camera I got for myself, and other spectacular figures. Thanks again!
Today, 26 years ago...
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Having a baby is one of the most wonderful things in your life, as well as the hardest thing in your life. ~ Nuno Bettencourt
Some weeks are harder than others. Some weeks, taking care of Corbin seems like this vacation from work on the Cayman Islands. The sun is shining and all is right in the world. Except for if that was true..I'd be using PAID time off...so it's not exactly like that ;) But those weeks are few and far between when your baby has colic. The weeks that fill in the gaps consist of these constant battles against yourself. Against your frustration, against your guilt, against your jealousy, and against your kicking, screaming, wailing baby.
I can't really complain. Our baby could be much worse off and things have been worse, but I might just be coming to the end of my rope. Might just be sick of this charade we put on everytime we feed our child. It's kind of like a marathon and you have no idea how many miles you are running. It's for a good cause, so you don't bother to ask because that would just be inappropriate. In fact, most people would think it was completely insane that any related thought had or will cross my mind. It's extremely hard to stay on course in the midst of a screaming baby that has run the gamut of soothing techniques with no relief. Standing in a dark bathroom, fan and water on full blast, covered in baby spit-up and bouncing on a large red exercise ball for what appears, at the time, to be for no reason...just might start to chip away at that perfect mommy exterior. The frustration, I'm assuming..is pretty self-explanatory. But then he flashes this sincere glimpse of his true temperament, this sweet little personality, and I forget that he was ever even crying. I remember why I'm running with no end in sight.
The colic isn't a disease he has, just a thing he does. His way of communicating that something is wrong. I hate it when people tell me that "he'll grow out of it" or that "he's just a baby and this is what babies do." A) I'm sure most of the people who make these lovely comments...have never had a baby with colic and B) Some of them don't have babies at all! I'm tempted to leave Corbin at their house for 48 hours and have them report back afterwards. Don't get me wrong..I love my child more than anything, but if this is what babies do...why the HELL would anyone have children?!?!?!? I'm not going to lie. The pregnancy was exciting for about 5 minutes and then I felt nauseous..literally. Then it was exciting again..then it got boring..then it got majorly uncomfortable. Not to mention the birth....when your epidural wears off just in time for the pushing. No one told me it didn't work for pressure. Thanks for that! Then he was cute for a few weeks during which I was nursing..some women love it, I was not one of those women..then after a few weeks, he started wailing. Now we are at the present.
Through all this rain, I can still see the reason for my endurance. No..I don't believe we are ingrained with a gene that makes us coo at babies even if we don't like them...this is from an article a coworker gave me on one of my last days at work..I'm not completely sure why. It's all the sweet, innocent moments you have in between all the chaos. Like these moments of clarity that come a little less often than the ones that make you feel like you might snap...but drown out all that white noise. In the past three months, Corbin has had jaundice, puked through his nose, puked on my face, pooped in the bathtub, cried what seems like at least 45 days straight, taken 2 hours to burp, caused 6 trips to the pediatrician and 1 to a gastrointerologist, made me sit in a car for an hour and a half just so he wouldn't wake up if I took him in the house, and let's not talk about what happens after you are done nursing ;) .....But GOD you should see him laugh :)
Richard and I were semi-obnoxious to my sister when my nephew was really little. She showed us his new trick...he could pick up a puff with two fingers and put it in his mouth. Seeing as we had not had children and were not near the stage...we were the least bit impressed. What the hell did we know?!?! Now I'm sorry I didn't see the amazement in what a huge accomplishment that was. It seems the longer we endure the "April showers" we're experiencing, the sweeter those moments of clarity, our "flowers", become. Corbin has started to reach for his toys and if you could only see the intent on his face. He is trying so hard to sit up that it causes him to spit up more, but I don't care (as long as it doesn't hurt him). Because he is my challenge and my reward all at the same time. He lights up when I walk into the room. I would sit in a bathroom for eternity just to see him smile once. This is why we have children. This is the break in the marathon that gives you the strength to keep on running. When he is all bundled up and I'm rocking him to sleep, I wish you could see him laugh at his dreams and then get quiet again as he drifts further to sleep. Those people who choose not to have children because "that is what babies do" are truly missing out on the reason for having them in the first place.


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The fastest live scores for your team!With this app you accompany your team to the 2018 cup in Russia !
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text:
The belt light flashes on,
everyone is seated and
preparing for takeoff.
The surface your body thought was safe
starts to shake.
Out the glass, a slideshow
racing too fast.
The front wheel lifts off the ground
pulling your body upwards,
thrusting your head against the seat
and the worries start to soar.
Now the thin glass is the only barrier
between you and the increasing height
and the cars on the roads below
are leaving your sight.
Dazing at the rows and rows of seats
you can't help but stare,
as the minutes turn into hours.
When the nose finally dips,
it's almost over.
The worst thing is
when your biggest fear
is the only way
to get you where you want to be.


text:
Strong running 1980 Triumph TR8 for rolling restoration
Price: US $3,500.00 Item location: Lake Forest, California, United States
- Condition: Used
- Make: Triumph
- Model: Other
- Type: Convertible
- Trim: Base Convertible 2-Door
- Year: 1980
- Mileage: 61,711
- Color: Persian Aqua Metallic
- Engine size: 3.5L 3528CC V8 GAS OHV Naturally Aspirated
- Number of cylinders: 8
- Fuel: Gasoline
- Transmission: 5 speed manual
- Drive type: RWD
- Interior color: Blue
- Vehicle Title: Clear Want to buy? Contact seller!
1980 Triumph OtherThe details here have changed - please make sure to read them carefully:
In the spirit of the current Star Wars buzz, he may not look like much but she's got it where it counts. She is only regrettably being sold help fund a Porsche 911 engine rebuild.
The car is a veteran of frequent SoCal TT and other touring events, nd is well known by that community of eclectic gearheads.
New front uprated springs from Ted, ew poly bushings everywhere including rear arms and swaybar bushings. The car is quite fast, andles and brakes well. She is an absolute blast on fast touring roads which is where I built her up to thrive.
All the parts are there for the power steering pump, o you can simply replace the pump to use the full power set up, ut as noted above it is currently disconnected as the pump was leaking. The steering rack was not leaking at the time the steering pump was disconnected. Carpet was ratty and was removed to wire brush and POR15 paint the cockpit floors. Sound deadening installed on top of rust free POR15 silver painted floors. The brake MC leaked which she was stored for a long time so there is a index size patch to the driver front floorboard. That was done 6+ years ago and no issues since.
No structural rust, ills heavily soaked in Eastwood anti-rust cavity wax, ockpit floors are great, runk floor is great, ender seams are great. Appears to never have been hit. Exterior sills had very-very minor surface rust on one side so both sides were stripped back to bare metal, reated with phosphoric acid wipe then Eastwood anti-rust black paint and crudely spray painted in the original color. As you probably guess I was planning on doing a bare metal repaint and was only focused on having a rust free shell rather than being focused on cosmetic until that point.
Headlights and turn signals and brake lights and horn and gauges all work properly. Clock is non-functional.
As noted above she has a 4.0 Rover engine with the correct higher compression 4.0 liter heads on her, he does not have the 3.5. This engine was rebuilt approximately 8 years ago and as under 9,000 miles on it since the full rebuild using a fresh block. All of the SMOG gear was transferred over, nd the engine has passed multiple SMOG visual inspections. The extra 1/2 liter of displacement and higher compression makes for a significantly stronger engine.
Mileage might be original and not flipped over based on the history that I have been able to document, he apparently sat for a long time in the 1990s and only had low miles from the immediately prior owner and myself. I am not sure why the original 3.5 liter engine was replaced, ossibly just the extra power but I don't have history on that.
Current on CA DMV non-op. No fees due.
Clear CA title.
Thick binder full of documentation including all work done and engine rebuild receipts and transmission installation and bushings etc that comes with the car.
Local inspections encouraged. Questions encouraged and will be promptly and honestly answered. Car is sold as-is with no warranties.
I would be happy to help with shipping but you need to arrange that please.
No reserve!
11/19/15 update: The car does not have air conditioning as it was not an air conditioning car from the factory. I added 23 additional pictures and have not dressed the car up in anyway, etter that than soaking it in Amour All and your getting a false impression. The carbs should be rebuilt from sitting, he runs fine but I believe one dashpot oil seal is gone and that is impacting throttle response. I provided close ups in the pictures where I had the front fender lips replaced due to that being a common rust trap, nly the lip area was replaced since the rest of fenders were fine. When I inspected her again this afternoon I noticed some evidence of a small coolant leak from the radiator. She runs dead center of the temperature gauge and has never overheated, or is she consuming coolant, ut you may want to pull the radiator at some point.


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"More Americans now work for government than in manufacturing."Now, whose fault is that?
Oh, and by the way, federal government employees receive an average compensation twice that of the average private-sector employee.
"More Americans now work for government than in manufacturing."Now, whose fault is that?
You and I both pay pretty close attention to school economics, although I'm newer to the game than you. One thing that has become clear to me over the past couple of years of immersing myself in this is that there is strong movement in our state to shift the school funding decisions from the voters of local districts to state-level elected officials - such as the State Bd of Ed, whose members we vote for but barely know.
The impetus is not because it's a better funding mechanism than what we have now, but rather because it essentially makes teachers employees of the State, and bringing them under the protective umbrella of state politics.
More importantly, it adds an other layer of indirectness to the consumer-supplier relationship which, contrary to Kathie Bracy's theory, makes the suppliers less, not more responsive to the consumers.


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April 25, 1997
VALLEY FORGE CENTER ASSOCIATES, APPELLANTv.RIB-IT/K.P., INC., APPELLEE
Appeal from the Order Entered August 28, 1995, In the Court of Common Pleas of Dauphin County, Civil, Writ of Execution No. 1154 N 1995. Before LIPSITT, J.
Appeal from the Order entered August 28, 1995, Court of Common Pleas, Dauphin County, Civil Division at No. 1154 N 1995, Writ of Execution. Before LIPSITT, J.
Before: Tamilia, J., Johnson, J. And Montemuro, J.* Opinion BY Montemuro, J.
The opinion of the court was delivered by: Montemuro
This is an appeal from the Order of the Dauphin County Court of Common Pleas denying the petition of Appellant, Valley Forge Center Associates ("VFCA"), for reconsideration of the June 23, 1995 Order which granted the petition of Appellee, Rib-It/K.P. Inc. ("Rib-It"), to stay and set aside a pending writ of execution. Because we conclude that this Court does not have jurisdiction, we must quash this appeal.
The facts as gleaned from the record are as follows. On August 16, 1994, VFCA entered judgment against Rib-It in the Court of Common Pleas of Montgomery County for past due rent and rent due pursuant to a sublease executed by Rib-It. On May 15, 1995, VFCA commenced execution proceedings by filing a Writ of Execution in Dauphin County and requesting that the County attach the liquor license owned by Rib-It and held by the Pennsylvania Liquor Control Board in Harrisburg. A sheriff's sale on the writ was scheduled to take place on June 26, 1995. Three days before the sale, however, on June 23, 1995, Rib-It filed a Petition to Intervene, Stay and Set Aside the Writ of Execution, alleging the priority of First Fidelity Bank's ("First Fidelity") perfected security interest in the liquor license. The trial court's Order of June 23, 1995 granting this Petition underlies the instant appeal.
On July 18, 1995, VFCA filed a Petition for Reconsideration of the June 23, 1995 Order. Thereafter, the parties filed briefs and the trial court scheduled the matter for a conference. On July 20, 1995, counsel for First Fidelity entered his appearance on the Bank's behalf, and, on August 8, 1995, filed a brief addressing First Fidelity's right to intervene and the priority of its security interest in Rib-It's liquor license. On August 28, 1995, the trial court entered an Order denying the petition of VFCA for reconsideration, and further ordered "defendant," Rib-It, to file an amended petition in accordance with Pa.R.C.P. 2328(a) controlling the procedure for intervention. *fn1 First Fidelity filed an amended petition on September 6, 1995 to which VFCA filed an answer on September 20, 1995. VFCA subsequently filed a notice of appeal on September 21, 1995.
VFCA ostensibly appeals from the August 28, 1995 Order denying its petition for reconsideration of the June 23, 1995 Order. Before we may address VFCA's challenges to the August 28, 1995 denial, however, as a threshold issue we must determine whether this Court has jurisdiction to hear the appeal. Although the jurisdiction of this Court was not challenged by either party, we note that this Court may raise this issue sua sponte. Rieser v. Glukowsky, 435 Pa. Super. 530, 535, 646 A.2d 1221, 1223 (1994, Accordingly, we must decide whether, under the applicable rules of civil procedure, VFCA properly preserved its right to appeal.
On June 23, 1995 the trial court entered an Order which stated:
AND NOW, this 23 day of June, 1995, upon consideration of Rib It/K.P., Inc.'s Petition to Intervene, Stay and Set Aside Writ of Execution, it is hereby ORDERED and DECREED that said Petition is GRANTED and the Writ of Execution is hereby STAYED and SET ASIDE.
Initially, we note that a court in which execution proceedings are pending has an inherent right to stay or set aside the proceedings where it is necessary to protect the rights of the parties. Pa.R.C.P. 3121; Keystone Savings Ass'n v. Kitsock, 429 Pa. Super. 561, 564, 633 A.2d 165, 167 (1993). The appealability of such orders, i.e., the jurisdiction of this Court, however, depends upon the character of the order, as the Superior Court has appellate jurisdiction of all appeals from final orders of the Court of Common Pleas. 42 Pa.C.S.A. § 742.
Generally, "'a determinative order relating to execution is in character a judgment, which, when of final nature, is appealable.'" Cherry v. Empire Mutual Ins. Co., 417 Pa. 7, 10, 208 A.2d 470, 471 (1965) (quoting In Opening of Parkway, 267 Pa. 219, 225, 110 A. 144, 146 (1920)). Typically, an order staying execution is deemed interlocutory, and, therefore, unappealable because it is granted to maintain the status quo pending the further Disposition of other court proceedings or investigations. Goodrich-Amram § 3121(c):5 at 246. See Cherry, 417 Pa. at 12, 208 A.2d at 472 (holding order staying execution interlocutory as it stayed execution "until further order" to permit interested party to enter appearance before court made final determination); Jenkintown National Bank v. T.L. Fulmor, 124 Pa. 337, 17 A. 2 (1889) (holding interlocutory a stay of execution since it was ordered merely to allow for further investigation of an issue before the court). An order staying execution becomes final and appealable, however, if granted for an indefinite duration. Cherry, 417 Pa. at 11, 208 A.2d at 471; National Council of Junior Order of United American Mechanics of U.S. v. Roberson, 214 Pa. Super. 9, 16, 248 A.2d 861, 865 (1969). Therefore, the finality and appealability of a stay order depends either upon the duration of the order or the reason for which it was entered. Cherry, 417 Pa. at 11, 208 A.2d at 471.
Alternatively, an order setting aside a writ of execution is a permanent remedy and constitutes a more drastic form of relief from execution. Goodrich-Amram § 3121(d):1 at 247. Accordingly, an order setting aside a writ is generally a final order subject to immediate appeal. Goodrich-Amram § 3121(d):6 at 251; Cherry, 417 Pa. at 12, 208 A.2d at 472. Cf. Rodgers v. Yodock, 309 Pa. Super. 154, 454 A.2d 1129 (1983) (holding unappealable order which "set aside" writ of execution since, by its terms, was not meant to be in effect for more than thirty days). Therefore, to determine whether an order ...
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SEGA Europe Ltd. today announced gamers can sample the highly anticipated Condemned 2 video game from esteemed development studio, Monolith Productions, via a demo released on the Xbox LIVE® Marketplace for the Xbox 360® video game and entertainment system from Microsoft®. Available for immediate download, the demo is taken from mid-way through the first level and gives players an opportunity to get to grips with Ethan Thomas, the former Serial Crimes Unit investigator, in his crusade to discover just what is going on in the sinister world around him.
I love the fact that Xbox Live is constantly updated with new content.


text: Select an international website Zoover.at Zoover.be Zoover.ch Zoover.co.uk Zoover.com.tr Zoover.com.ua Zoover.cz Zoover.de Zoover.dk Zoover.es Zoover.fr Zoover.gr Zoover.hu Zoover.it Zoover.lt Zoover.nl Zoover.no Zoover.pl Zoover.pt Zoover.ro Zoover.ru Zoover.se Home Destinations Accommodations Awards Add review Add review Add photos Add videos Add new accommodation Add attraction Add acco information Home Add review Spain Catalonia (Costa Brava and Costa Dorado) Barcelona Aparthotels Barcelona Aparthotel Majestic & Spa***** Popular destination Guaro Cala San Vicente Playa de las Americas Lloret de Mar Barcelona Maspalomas Alcudia / Puerto de Alcudia Costa Adeje Malgrat de Mar/ Santa Susanna Tàrbena Popular destination Lloret de Mar Barcelona Malgrat de Mar/ Santa Susanna Pineda de Mar Salou Platja de Pals / Playa de Pals Macanet de la Selva Playa d'Aro L'Estartit Sant Cebria Popular in Barcelona Apartments in Barcelona Hotels in Barcelona Attractions in Barcelona Aparthotels Catalonia (Costa Brava and Costa Dorado) Aparthotels Spain Reviews Aparthotel Majestic & Spa ***** Reviews Photos & videos Tips Question and Answer Weather Aparthotel info Map 9.1 Unfortunately no photos available Add the first photo 2 American reviews 40 international reviews Find Hotels Deals for Majestic & Spa***** From € 287 Find the best deal 13° / 21° 21 Apr 2018 Weather Number 1 of 20 aparthotel in Barcelona Find the best deals Most visitors found the best deal for Aparthotel Majestic & Spa***** with: 1 Booking.com Check price tip More suppliers Collapse 2 American reviews Aparthotel Majestic & Spa 8 2 Reviews Appreciation 8 Location - Service - Value for money - Food - Rooms - Child friendly - Swimming pool - Hygiene - Language: American (2) All (21) ------------ Dutch (16) German (6) English (5) French (4) Spanish (3) Italian (3) Portoguese (3) Party types: All (2) ------------ Sort by: Date of visit Contribution date Great location and breakfast buffet 10 Joseph, 25 June 2009 Location - Service - Value for money - Food - Rooms - Child friendly - Swimming pool - Hygiene - Great location, convienent to all sights and near Barcelona Bus Touristic. Room small but clean and well equiped. Would definitely recommend to other travelors. _._ 6 robert, 25 June 2009 Location - Service - Value for money - Food - Rooms - Child friendly - Swimming pool - Hygiene - There are two buildings included in this Hotel.One building has not been refurbished. I was initially booked into this builder. They changed my room the next day. In the unimproved building, the halls, rug and general appearance was very disappointing. The room was dark (LITTLE SUN) AND THE VIW WAS OF MY NEIGHBORS UNMADE BED.The newer building, though the rooms were small, was much improved Would you like to know what to expect on your vacation in Aparthotel Majestic & Spa? Then have a look at the reviews of Aparthotel Majestic & Spa in Barcelona, written by other travelers. Aparthotel Majestic & Spa scores a 9.1 out of 10, based on 21 reviews read more Would you like to know what to expect on your holiday in Aparthotel Majestic & Spa? Have a look at the reviews of Aparthotel Majestic & Spa in Barcelona, placed by other travellers. Besides that you will be able to find 4 vacation photos of Aparthotel Majestic & Spa on Zoover. You can take a look at 2 videos of Aparthotel Majestic & Spa. - - - Less text Actions for Aparthotel Majestic & Spa***** Add review Add photos Add videos Add new accommodation Add attraction Add acco information Print reviews RSS feed Attractions in Barcelona La Sagrada Familia Parc Guell Stadion FC Barcelona Casa Batllo Casa Mila - La Pedrera Barri Gotic Mercat de La Boqueria Information about Barcelona Weather Barcelona Map Barcelona Photos Barcelona Videos Barcelona Question and Answer Barcelona Are you the owner? Create a Zoover Account and manage your accommodaton, attraction, or restaurant. Click here for more information Zoover link on your website Click here for all Zoover widget options


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