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Czech Republic > Prague
Alternatively, you can create a request.
|Hourly Rate
|Angelina Love
, 26, Prague
HELLO GENTLEMEN, I have strong credentials in the porn movie business, having made over 50 porn movies, therefore a very experienced provider on the technical side. As opposed to other escorts claiming to be able to provide you with a special service (the porn star experience) when it comes to private time, I don't make promises, I deliver. Special desires get fulfilled and special fantasies acted upon. Let it be role play, fetish, domination, or strap-on arrangements. I'm not just very open minded but have the know-how and expertise in all of these areas,so you are in safe hands. -At the same time, I keep a very low profile on my porn star status. I never appear on public events or promotions, my face is not well known to the public, so you will never get into an uncomfortable situation when going out with me. Also, I guarantee that you will never feel intimidated or uncomfortable in our private time, as I always adjust to your own level, speed, needs and requirements and only introduce you to new experiences if you want me to. I will never put on a show, I genuinely enjoy the time spent together with my partner. -In addition, in my private life I'm the most charming and easy going, caring and giving. I was told several times by my very appreciated regular customers that the reason they've chosen me is my warm and affectionate character, natural smile, big heart and alluring nature. It is very easy to bond with me and they can relate to me as a friend. -Last, but not least, what is making the biggest difference to the mainstream market is the special emphasis I put on a highly elegant, graceful and tasteful appearance. I chose my clothes with special care and reinvest a considerable amount of my earnings into looking exquisitely classy and elegant. I only go for high quality, beautifully cut fabrics and pieces, classical black pant suits, skirts, beautiful shoes and accessories, so whenever you have to appear with me in public you will have a classy, refined and elegant lady on your arm as opposed to a cheap agency escort making you feel ashamed. I hope that the above information was useful to decide if I'm the right lady for you. I would love to make your stay in Budapest a very pleasant and pleasurable one. Bookings by email should be done through my site.
|250 €
|Alla Cdc
, Prague
Escort Agency Hello I am Alla I am a new girl hot baby, very pretty and ready for all about your wishes. I will show you how to get fun and be sexy at the same time and I know how to do it!!! So, I am waiting for you text me in whatsapp and I promise that I will be unforgettable for you.
|Vikki Cdc
, Prague
Escort Agency Hello. I am VIKKI, I am pretty, tender, caring, loving and very passionate girl and high-class companion. Would you like to forget about your problems, disconnect from your daily routine? It s very easy – just book me and I will make you the happiest man in the world ! You will see that I am very mild, charming, caring and loving pussy kitten.... I will cover you with sweet kisses all your body – and between my firm warm breasts – you will feel like in the paradise ! I am waiting for you – in my best sexy and appealing lingerie – for an unforgettable hour of relax and pleasure !
|Lana Cdc
, Prague
Escort Agency Hello Gentlemen,my name is Lana. I am a young and attractive looking lady and an Elite Companion with a best for all the good things life provides for those prepared for success. I consider myself as a real woman, with perfect body curves beautiful, soft skin and perfect to touch… something I am particularly proud of. If you appreciate discretion and confidentiality, and are looking for a sexy, sweet, intelligent companion who provides the ultimate experience, I am the one for you! I have impeccable manners, grace and charm. you would not regret seeing me!!
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If you're one of the nearly 70 percent of adults in America who struggles with their weight, you probably don't need us to tell you that losing those extra pounds isn't as simple as it seems. Low-fat, high-fat, all carbs, no carbs—the number of fad diets and supposed "quick fixes" out there are endless, but they often don't work and usually leave us feeling frustrated and hopeless.
It should be easy, right? Decrease calories and increase calorie expenditure, and the weight should just melt off. Mathematically, the "eat less, exercise more" formula is easy enough to understand, but in reality, our human bodies are incredibly complex, and we have to take much more into account than just how much we eat and exercise when it comes to sustainable weight loss.
Although many factors play a role in your weight—what you eat, how you sleep, your stress levels, and your lifestyle, to name just a few—the foundation of your metabolism actually resides in your gut. And, improving your gut health from the inside out may be just what you've been missing in your efforts to attain and sustain your ideal weight.
What is Gut Health?
When we talk about gut health, we aren't referring to the size of your waistline. In fact, we're not even talking about any of your human cells or organs—we're speaking about all of the microorganisms in your digestive tract that make up your gut microbiome.
Yes, it's true—your body is home to trillions of microbes, most of whom work on your behalf to keep you healthy. Among many other things, the beneficial bacteria in your gut work to help you digest foods, regulate your immune system, help you sleep, and even keep your emotions in check.
In fact, these probiotics are key to so many bodily processes; it's no wonder that they have a major influence on our metabolism and weight as well. Here's how:
• Supporting our blood sugar control. Proper blood sugar balance is critical to a healthy weight. Why? On the one end, fluctuating glucose levels can lead to unhealthy food cravings for sugary foods that will give us a quick energy boost. At the other end of the spectrum, excess glucose gets stored as fat, but an influx of insulin (in response to the extra glucose) turns off our body's signal to burn that fat. Probiotics work to balance our blood sugar by increasing our body's sensitivity to insulin and increasing insulin secretion when necessary1.
• Increasing our nutrient absorption. Our good guy gut bacteria work hard to help break down and digest all the foods we eat, and they produce short chain fatty acids, enzymes, and vitamins that are integral to the digestive process. But, without enough friendly flora to do the job, we can't absorb all the valuable nutrients we need—no matter how healthy our diet is. When we aren't absorbing our food properly, even though we are eating plenty of calories, our body never feels satiated and we are constantly hungry and craving all those missing nutrients. Research shows that an unbalanced microbiome (lacking in probiotics) can even lead to malnutrition2.
• Regulating our hormones. The good microbes in our gut help to support many of our body's hormones, like leptin, a hormone made by our body's fat cells that determines our feelings of fullness. Ideally, leptin is supposed to signal our brain that we are full and should stop eating, but our body can become resistant to leptin, and our brain—thinking we are starving—tells us to eat, eat, eat! The good news? Probiotics increase our body's leptin sensitivity, enabling our brain to get the "I'm full" signal3.
• Reducing our stress. Stress can wreak havoc on our weight and overall health. You see, not only can stress majorly deplete the beneficial bacteria in our gut—leading to problems with digestion, nutrient absorption, hormone regulation, and weight gain—but stress often causes us to overeat carb-heavy comfort foods that will give us a temporary (and calorie-rich) mood boost. Fortunately, probiotics can reduce levels of cortisol, the "stress" hormone, and they produce a vast majority of our body's serotonin, the "happy" chemical4.
Probiotics and Weight Loss
So, we know some of the "how" when it comes to probiotics and their effect on our weight, but how do we know they actually work? Luckily, you don't have to take our word for it—research shows that probiotics can positively impact both weight and BMI (body mass index).
In one study, women taking daily probiotics for six months saw significant body weight and fat mass reductions compared to women taking a placebo5. In another recent review of 2,000 adults, researchers discovered that consumption of probiotics reduced both BMI and weight in subjects, especially for those taking multi-strain formulas for at least two months6.
Scientists still have much to discover when it comes to the microbiome and the many ways it can affect our weight and metabolism, but they do know that people who are overweight tend to have different microbial compositions than people who don't struggle with weight issues7. The reason could be that certain less friendly microbes (usually abundant in overweight individuals) produce massive amounts of short chain fatty acids, which add to our daily calorie absorption.
The good news is that adding in probiotics to your health regimen is a simple way to achieve proper microbial balance in your digestive tract which will help you get both your metabolism and your weight back on track.
Increase Your Gut Health, Decrease Your Waistline
If you want to shed a few pounds, research shows that taking care of the microscopic friends in your gut is your best first step. Here are our tips for living a gut-healthy life:
1. Nurture your gut. A healthy gut environment will enable your good guy microbes to flourish so they can get to work supporting your weight.
• Take a high-quality daily probiotic formula like Hyperbiotics Better Body to seed your gut with billions of beneficial bacteria that can work to optimize your metabolism at its very core.
• Load up on fermented foods for a powerful probiotic boost. Kombucha, sauerkraut, and kimchi are all great sources of friendly flora.
• Feed the probiotics in your gut with prebiotics, indigestible fibers that are your belly bugs' favorite food source. Indulge in honey, oats, bananas, asparagus, and onions—and add in a daily prebiotic powder supplement for maximum benefit.
2. Stay away from probiotic killers. Unfortunately, today's society is rife with chemicals, toxins, and medications that are deadly to the good bacteria in your gut.
• Avoid antibiotics when possible, both as medicine and in the food supply—they indiscriminately wipe out the good guys along with the bad, depleting your precious microbiome and making it much easier to pack on pounds.
• Steer clear of antibacterial cleaners and chemicals, which also kill the good bacteria trying so hard to set up shop in your body. Look for natural cleaners like gentle soap, water, baking soda, and apple cider vinegar.
• Limit your exposure to environmental toxins. Not only do these harm your beneficial microbes, but they can also disrupt your hormonal balance, leading to unwanted weight gain.
• Skip the artificial sweeteners! Although often used in drinks and snacks to quickly cut calories, non-caloric artificial sweeteners can have disastrous effects on your microbiome and your glucose metabolism8.
• Focus on a diet rich in whole, unprocessed foods, and aim for at least 60% fruits and vegetables.
3. Get active and stress less. We know that stress impacts the microbiome, and research shows that physical activity benefits more than just your muscles.
• Engage in moderate exercise. Studies show that active people have more diverse, healthier microbiomes than those who are sedentary9.
• Relax your body and mind with meditation, yoga, or your favorite stress-busting practice—your microbes will thank you!
• Get outside and enjoy nature for a healthy dose of bacteria. Exposure to all kinds of microbes in the environment strengthens your immune system, and getting outside can help you unwind after a busy day.
While diet and exercise are important facets of any weight loss program, we must take a holistic approach when it comes to optimizing our body's metabolism. In fact, in recent years we've learned that one of the reasons that diet and exercise are so integral in achieving our ideal body weight is because of their effect on our microbes! That's why taking care of your gut health and nourishing your beneficial bacteria so they can fully support all of your intricate metabolic processes is foundational when it comes to experiencing vibrant health and sustainable weight loss.References:
Emily Courtney is a Writer and Editor at Hyperbiotics and mom to two fun and active boys. Emily is passionate about natural wellness and helping others learn about the power of probiotics for vibrant health! For more ideas on how you can benefit from the power of probiotics and live healthier days, be sure to subscribe to our newsletter.
This Healthy Living section of the Hyperbiotics website is purely for informational purposes only and any comments, statements, and articles have not been evaluated by the FDA and are not intended to create an association between the Hyperbiotics products and possible claims made by research presented or to diagnose, treat, prevent, or cure any disease. Please consult with a physician or other healthcare professional regarding any medical or health related diagnosis or treatment options. This website contains general information about diet, health, and nutrition. None of the information is advice or should be construed as making a connection to any purported medical benefits and Hyperbiotics products, and should not be considered or treated as a substitute for advice from a healthcare professional. Always seek the advice of your physician or other qualified health professional with any questions you may have regarding a medical condition.

subset: commoncrawl


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Utah Jazz: Earplugs anyone? Nets' Kevin Garnett is in town
Jazz Source Alpine, UTFeb. 19, 2014 12:59 p.m.
Trash talking is great. It adds to the entertainment value of the game.
Trade deadline?
The Jazz need to get on with it!!
This team is winning too much right now. They are not a playoff team AND they are not going to be a high lottery pick team.
Pointless. Wasted losing. Squandered upcoming draft.
Yes I know there are no guarantees but odds are better with more talent available.
Lindsey and OConner have done some things well but they need to seize the moment. They need to be bold and deal some players who are not enough of a difference-maker to matter if traded.
Hayward is a tough decision. All around good player/guy. Franchise player? Nope. Too many years into his career to believe there is still big upside. He can improve but not tremendously. Deal him if 1st round pick(s) are in the deal.
Sit Favors for weeks until he heals. More losses.
Trade Jefferson and/or Williams. More losses.
Trade Kanter for a player who can defend the stretch4 position or picks. More losses.
Make some moves for a chance get us out of mediocrity!
Drafting a franchise player is our only hope!
Iowa Jazz Fan 2 Cedar Rapids, IAFeb. 19, 2014 8:49 a.m.
RE the rumor that Jazz might be willing to trade Hayward to Boston for one of the Celtics' future first round picks. Hmmmm . . . . . So they would be willing to give up a past first round pick, now in his fourth year of development and the team's leading scorer, and a proven playmaker, for an unknown first round pick???
Hmmmm . . . . I'll chew on that one. At the moment I can't see much sense in it.


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Photo #3
exposure: 1/100sec f/stop: 5.6 iso: 200
After discussing a few ideas with Marione today, JMCK will definitely have a shoot sometime next week. I won't say what our theme will be but it should be pretty cool.You'll see our finished shots when we're done and happy with them. I don't know if a couple of members know if I even have a blog yet but if you're reading this, expect a few messages on details about next week from me. Also I think its time for a group meeting.
Doing a few studio shots and experimenting with my flashes is pretty fun. I'll do my first tutorial tomorrow on different types of flash techniques and wireless flash too. Any suggestions on any other type of tutorial would be welcomed as well. I wish I had a proper video camera though cause it would be so much easier to record and upload to youtube. Oh well in time it'll happen. And I also plan to do a review of one of my lenses soon. The Tamron 17-50mm 2.8, which is available in Canon, Nikon, Sony and Pentax mounts.
Also I have some news that I haven't really told anyone. A online tourist guide site called Schamp emailed me one day and asked if they could use one of my photos for their tourist guide from Sculptures by the sea. I straight away said yes and though I didn't get paid for it, my photo is credited and its a good opportunity to get my name out there. That was pretty exciting. Makes me want to do more events and do some freelance work.
PMA is coming up soon! Can't wait to see all the new gear that gets announced. Hoping for new sony lenses and a700 replacement. *fingers crossed*
That's all the news for now.
Take care everyone and thanks for reading =)


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Michael Mandarano is a freelance copyeditor, proofreader and formatter, and (full disclosure) a former colleague of mine from Harlequin Enterprises. Last year, Michael proofread Her Son's Hero and also helped Wynne Channing format her book What Kills Me. I've asked him to tell us about what a copy editor can do, especially for authors looking to self-publish.
So you've downed that eighth cup of coffee, pulled an all-nighter and finally completed your work in progress. Your baby is complete. So what now?
Well…you could run a spell-check, close the file and start figuring out how to get your masterpiece into the hands of readers. Yes, you could do that. But therein lies the most common mistake made by indie or self-published authors and, in my opinion, the most damaging one to make.
The manuscript you've just poured your heart and soul into may very well be the next Fifty Shades of Grey or Beautiful Disaster, but without a trained and focused eye (and one that's a bit more removed than yours), it's bound to contain typos, inconsistencies and other errors that have slipped through the spell-check cracks.
In short, you need a copy editor.
As a copy editor, my job is to take a completed manuscript and smooth out the wrinkles. By this stage, you and your editor (make sure you have one of those, too) will have worked out the major issues in your story: plot and character development, proper use of dialogue, showing versus telling, and so on. Focusing on these top-line elements is extremely important, but it also leaves lots of room for smaller errors to remain undetected. In fact, often the editing process itself inserts errors into the manuscript that weren't there before.
A good copy editor will go through your work and catch errors of spelling, grammar, punctuation, inconsistency and awkward phrasing. It's what we do. Some like to call us detail-oriented, while others prefer the term anal. I prefer the first! 🙂 A good copy editor will do all these things while ensuring your author voice remains strong at all times. It's your book; we're here to give it that polished and professional look that readers have come to expect.
So once you've made the smart decision to hire a copy editor, what can you expect?
First off, I usually ask for the completed manuscript to get an idea of what level of copyediting is required, and to give an estimate of the total cost. I provide competitive per-hour rates and complete honesty when pricing projects. No one likes invoice shock!
Depending on the length of your book, my standard turnaround is usually one to two weeks. At the outset, we'll discuss your preferences regarding spelling (American, British, Canadian) and whether you prefer any particular dictionary or style guide.
I copyedit most often in Microsoft Word using the Track Changes feature. It's the easiest way to edit a manuscript with complete transparency and, combined with the Comments feature, allows me to explain any changes I make, as well as add suggestions here and there. For those using Word alternatives (e.g., OpenOffice or Pages for Mac), no worries — they're compatible!
When you receive the marked-up file, you'll go through the manuscript and choose to either accept or reject my edits and read the comments throughout. If you have any questions at that point, send them along!
And that's it! You'll have a polished, ready-to-be-published manuscript that you'll be proud to present to readers.
Next up: formatting and producing an ebook. Stay tuned for my upcoming post on this topic…
For a limited time, I'm offering discounted copyediting rates to authors in need of my services. Drop me a line for details, and be sure to mention this post. And stop by michaelmandarano.com if you'd like more information about me and my work.
Best of luck!


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Part of Calphalon's premium Tri-Ply Copper line, this brilliant stainless-steel and copper-accented cookware cooks beautifully and looks gorgeous. The pan's exterior is crafted of copper--the superior metal for heat conduction--while a heavy-gauge aluminum core runs through its center. The combination of these two metals provides more exact control while cooking and excellent end results. The interior is made of 18/10 stainless steel, which is nonreactive to acidic foods and offers a reflective surface that makes it easier to know when foods are completely cooked. This cookware has Calphalon's patented stainless-steel Cool-V handles that stay cool to the touch on the stovetop and stainless-steel lids that fit snugly onto the pan for a tight seal. All Tri-Ply Copper cookware is oven-safe up to 450 degrees F and is covered by a lifetime warranty. --Cristina Vaamonde
What's in the Box
8-, 10-inch omelet pans; 3-quart covered saute pan; 1-1/2-, 2-1/2-, and 6-quart covered saucepots


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Delivery Information
Spend $200 & get free delivery to most of Australia
Click here for all Australian freight rates
- Melbourne 3-4 working days
- Sydney 4-5 working days
- Brisbane 4-6 working days
- Adelaide 4-5 working days
- Perth 5-10+ working days
For express service, call 1800 069 295 for a quote.
International deliveries click here We cannot ship to all countries.
There are currently no reviews for this product.
2014 Zonte's Footstep Doctoressa di Lago Pinot Grigio
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- Cellar Drink Now (2015)
- Closure: Stelvin
Brilliant very pale straw, almost water like colour with greenish tinged edges and a watery hue. Ripe pear and red apple aromas are overlaid by a hint of musk and faint spice. The palate has good intensity with flavours of pear and red apples dominate over a touch of citrus with a dry spicy finish. Good textural feel. Medium to long aftertaste of pears, red apple and spice. A delightful Pinot Grigio.
Drink now.
Alc 13%


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Two men charged with shooting guns illegally
Mark Wilchinsky, of Stratford, and
According to police, the two men decided to shoot on the property of one of their girlfriend's mothers, but neither received permission from her to do so. A neighbor called the police.
Wilchinsky and Strauss were charged with unlawful discharge of firearms, breach of peace, third-degree criminal mischief and discharge of firearms within 500 feet of a building, which is against a town ordinance.
Both were released on written promises to appear in Danbury Superior Court next Tuesday.


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bitComposer Entertainment has announced the upcoming release of Air Conflicts: Vietnam for a variety of platforms including Mac. The Vietnam era aerial combat game is the latest installment in the Air Conflicts series which combines both flight simulation and arcade elements
Air Conflicts: Vietnam offers the popular combination of flight simulation and arcade gameplay. In the course of the game, the player controls over 20 accurately simulated fighter jets and helicopters from the Vietnam War era. In certain missions, the player has to leave the pilots seat temporarily to operate the guns and defend against attacks. If several fighter jets take part in a mission, the player can equip and upgrade them individually, as well as control the entire squadron. Air Conflicts: Vietnam offers a variety of missions and a compelling story set against the background of one of the best-known and most controversial wars in modern history.KEY FEATURESThere cant just be winners in war! Immerse yourself in the compelling and emotional story and experience, alongside heroic moments, the darker side of war.Experience the Vietnam War, one of the most explosive and violent wars of all time, up close! Witness the experiences of a brave U.S. Air Force pilot and immerse yourself ever deeper in the story thanks to the dramatic musical score.In addition to fighter jet missions, there are also new, special helicopter missions to complete.Take control of entire squadrons! Control all of the airplanes in your squadron.Equip your airplanes and helicopters with realistic weapons! All possible combinations of airplanes (or helicopters) and weapons are based on real models.A realistic setting combined with arcade gameplay! Every airplane can only be loaded with a limited amount of ammunition. However, you can compensate for this by switching between different airplanes in your squadron.A game that never gets boring! Play different single-player and innovative multi-player modes.Choose from a massive arsenal of military machinery! The game includes over 20 different airplanes and helicopters from U.S. and the Russian side.
Recent Mac Games News
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Search for other Mac games news stories or browse our Mac Games News Archive.


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Event Archive - AUDIO GEOMETRY: Moka Only, Lucid AfterLife, Wes Regan, Lukifero
Sat. February 28th 2015 @ Fox Theatre (No Minors)
7:30 - 10:30 doors at 7:00
Tickets at: Banyen Books
Presented by: THE LODGE OF EDUCATION AND RESEARCH
ReviewsNo articles have been linked to this profile
Moka Only "Daniel Denton ex-Swollen Members"
Daniel Denton ex-Swollen Members more info
Lucid AfterLife (Metal/Hip Hop/Blues/Rock/Funk/Whatever We Want/)
If you have not heard of a Lucid AfterLife then you have not had your ears to the Streets in Western Canada, your also missing out on a one of a kind musical, theatrical and emotional experience that can only be delivered by the boys of Lucid AfterLife. Having been together for just a single year they have toppled the Vancouver scene, packing venue... more info
Audio: Lilith, I Am, Alpha Omega


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Free Office Without The Microsoft—ThinkFree Online
Posted on 27th April, 2006
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Are you looking for an alternative to the Microsoft office apps? Look no further because here comes ThinkFree Online.
ThinkFree Online is the free web-based office apps which allow you to do nearly everything you could with Microsoft Office but all for free.
Extreme Tech has posted an in depth look at this newly updated webware at their site.
Today ThinkFree releases a major update to its suite, upgrading free online storage space from 30MB to 1GB and adding a new lightweight AJAX-based collaboration feature and the ability to apply folksonomy to a document through "tagging" (very Web 2.0). Another Web 2.0 feature of the suite is Mashup—when a web application incorporates functionality with other web services. In ThinkFree's case, the combination is with Flickr for inserting pictures into documents now, and later the company plans integrating Google and Yahoo for maps, and with del.icio.us for shared bookmarking.
This service is ad supported, but you can upgrade to the ad free premium services if you are willing to spend some money. In short, it offers excellent Microsoft Office compatibility and includes most of the Office features. To top it off, it also comes with a free 1GB online storage too.


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Welcome to The DynexHobby webstore. DynexHobby sell innovative instruments for hobbyist, educational institutions, reasearch and mechanically minded.
Please select the product you wish to purchase in the "Catergory" menu. All items are shipped from Australia. Please note, our courier does not ship to PO Box addresses.
Thank you for shopping with us.
Shopping Cart Software by Ashop


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Dover Street Market
We know your quandry: you only have a day in London and you want to catch up on the quirky fashion styles that the city is made for. Luckily there's a one-stop shop that can give you a taste of that elusive London flavour in one go. It's actually the flagship store of hip Japanese label Comme Des Garcons but it stocks a huge range of other designers too. Look out for top Brit designers like Christopher Kane and JW Anderson, browse through hard-to-find international accessories and design pieces, and be sure to visit the Nike Lab in the basement – home to some ultra-rare and very cool trainer designs. Even if you're not spending it's worth visiting just for the beautiful Japanese shop design.
P.S. if you've been out of London for a bit you might be be slightly confused to find out that Dover Street Market isn't in fact in Dover Street at all any more!
Where: 18-22 Haymarket, SW1Y 4DG (Entrance on Orange Street)
When: 11am-7pm Monday to Saturday, 12pm-6pm Sunday
Tel : +44 20 7518 0680
Hackney Walk
No it's not some music-hall dance, the Hackney Walk is instead quickly turning into the best place in east London to shop for British designers. What started as a loose cluster of outlet stores set in converted railway arches has grown into a whole fashion district. You really get the feeling that you're supporting the best of British when you're shopping here with the likes of Burberry, Aquascutum and Pringle all on display, and summer of 2016 saw a host of new stores, designed by award-winning Brit-architect David Adjaye, which upped the fashion ante. Look out for London fashion taste-makers Matches whose store here is a great capsule collection of what's hot in the city right now.
Where: 145 Morning Lane, E9 6LH
When: 10am-6pm Monday to Saturday, 11pm-5pm Sunday
Travel: Hackney Central train
On a good day at Aida you can buy something new from an up-and-coming east London designer, get your hair done in a genuine 1940s style, browse accessories from watches to books to go with your new look, pick up a bag to keep all your new purchases in and see a band. All without leaving the shop. because Aida is less a boutique, more a neighbourhood hang-out. It's a carefully curated collection, focusing on simple, beautiful European brands that go together well so you can put together a whole look, and with fashion events, DJ nights and even live bands there will somewhere close at hand to show off the new you.
Where: 133 Shoreditch High Street, E1 6JE
When: 10.30 am – 7 pm Monday to Saturday, 12 am – 6 pm Sunday
Travel: Shoreditch High Street underground
Tel: 020 7739 2811
Bicester Village
Qbic's in London. Some of the world's best fashion is in London. So why are we recommending a shopping destination 60 miles away in a sleepy countryside village? Because Bicester Village is where Londoners go for their luxury bargains. And village is a fair name for it – this is more than an outlet store, this is a whole street packed with some of the most desirable brands in the world. There are too many to mention here so check the website for details but Prada, Gucci, Burberry are just some of the big names that have huge shops here selling end-of-line classics and some lines made specially just for Bicester. There's a swathe of great food places too, and with an additional 10% off for Qbic guests it's worth making one of your days in London a Bicester shopping excursion. (Just ask at reception for details on how to get your extra 10% off!)
Where: 50 Pingle Dr, Bicester OX26 6WD
Travel: See website
If you want that genuine east London look then Goodhood's your place. Shoreditch's favourite shop got so popular that it had to move up the road to this new two-floor Curtain Road site where it expanded its range of quirky clothing and accessories. Expect retro Ts, slouchy pants, cult Japanese brands and left-field sportswear (think more mountaineering gear than jogging!) It's also home to a rather nice cafe and a beauty section that steers clear of the big names for some hard-to-find grooming brands. A one-stop shop for the east London look.
Where: 151 Curtain Road, EC2A 3QE
When: 10:30 – 18:30 Monday – Friday, 10:30 – 19:00 Saturday, 12:00 – 18:00 Sunday
Travel: Old St underground and train
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I have lone stiff-backed up one point on my computer-my iPod library. This is because my machine suggested that I posterior up my iPod music. I did it because I had bought numerous songs on iTunes and I looked-for to have my songs hardbacked on CDs in legal proceeding a virus derelict my information processing system. However, after taking the instance to flame a heap of CDs, I granted not to nick the juncture to wager on up my unbroken computer because I knew it would help yourself to too long-run. Who requirements to sit in front of their computer for a few days to section CDs and expurgate turn CDs? However, after my virus trust expired, I settled to fix your eyes on into discovery a amended posterior up policy for my computing machine files.
My crony advisable that I buy an external problematical drive, but it seemed too pricey on my learner fund. I began probing for new options and I saved an online accumulation association that measured elementary to use. Luckily, it was unhampered too! Since I don't have a lot of juncture or money, I rapidly downloaded the software, installed it, and stiff-backed up my files within your rights then.
It straight-backed up 2 GB and keep it for as drawn-out as I desirable. I decided that I would go to vast retention for about $5 per month if I necessary to.Post ads:
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text: Sorry, I can't find the original advisory, but Bugtraq 25277 looks bogus to me. Looking at the affected file in either OpenAds 2.0.11-pr1 or phpAdsNew 2.0.4 For example, the "affected" code for OpenAds 2.0.11-pr1 appears to be this: ---- snip, snip, snip ---- function phpAds_geoLookup() { global $phpAds_config, $phpAds_geoPluginID; if (!$phpAds_config['geotracking_type']) return; // Load plugin $phpAds_geoPlugin = phpAds_path."/libraries/geotargeting/geo-".$phpAds_config['geotracking_type'].".inc.php"; if (@file_exists($phpAds_geoPlugin)) { include_once ($phpAds_geoPlugin); ---- snip, snip, snip ---- The affected variable is only used in this function call. While the function is indeed called within the script, I don't see how an attacker can control the value via the 'phpAds_geoPlugin' parameter. Or am I just missing something? George -- theall at tenablesecurity.com


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text:
Google's financial results for the fourth quarter of 2013 indicate that the company made $16.86 billion in revenue during the period in question.
The figure marks a 17% increase compared to the same period in 2012, while net income rose from $2.89bn to $3.38bn.
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However, Google also revealed that its Motorola division - which has since been sold to Lenovo - lost $384 million during the quarter and haemorrhaged $1.25bn during 2013.
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text:
In this article we're going to check gaming performance of Intels latest Sandy Brdige E flagship CPUs when they're being supported by a Radeon HD 7970 graphics card from XFX. We're curious if there will be a measurable performance gap between these to CPUs regarding modern games or if the 100 MHz and three megabyte more Cache, which the Core i7-3960X has to offer, aren't enough to make a difference.
Del med dine venner


text:
BioMed Research International
Volume 2013, Article ID 352370, 7 pages
Cardiac Fibroblast-Derived Extracellular Matrix (Biomatrix) as a Model for the Studies of Cardiac Primitive Cell Biological Properties in Normal and Pathological Adult Human Heart
1Department of Public Health, University of Naples "Federico II", Via S. Pansini 5, 80131 Naples, Italy
2Department of Cardiothoracic Sciences, Second University of Naples, Via L. Bianchi, 80131 Naples, Italy
Received 23 February 2013; Accepted 11 April 2013
Academic Editor: Martin Götte
Copyright © 2013 Clotilde Castaldo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Cardiac tissue regeneration is guided by stem cells and their microenvironment. It has been recently described that both cardiac stem/primitive cells and extracellular matrix (ECM) change in pathological conditions. This study describes the method for the production of ECM typical of adult human heart in the normal and pathological conditions (ischemic heart disease) and highlights the potential use of cardiac fibroblast-derived ECM for in vitro studies of the interactions between ECM components and cardiac primitive cells responsible for tissue regeneration. Fibroblasts isolated from adult human normal and pathological heart with ischemic cardiomyopathy were cultured to obtain extracellular matrix (biomatrix), composed of typical extracellular matrix proteins, such as collagen and fibronectin, and matricellular proteins, laminin, and tenascin. After decellularization, this substrate was used to assess biological properties of cardiac primitive cells: proliferation and migration were stimulated by biomatrix from normal heart, while both types of biomatrix protected cardiac primitive cells from apoptosis. Our model can be used for studies of cell-matrix interactions and help to determine the biochemical cues that regulate cardiac primitive cell biological properties and guide cardiac tissue regeneration.
1. Introduction
Advances in stem cell biology and recent reports of stem cells and progenitors cells residing in the adult human heart gave origin to cardiovascular regenerative medicine, and this approach to the heart failure treatment immediately captured the attention of clinicians [1]. Numerous preclinical studies and clinical trials have aimed at the use of stem cells in the therapy of infarcted heart or ischemic cardiopathy [2], evolving from cell therapy to refined bioengineering. In the era of tissue engineering, there is an ongoing interest in the development of biomimetic materials that would be able to elicit specific cellular responses and direct tissue formation based on either intrinsic tissue regeneration capacity (provided by resident stem cells) or scaffold-incorporated or scaffold-seeded stem cells [3]. Several such approaches to cardiac tissue regeneration have been developed and are still under investigation (reviewed in [4]).
All cells exist in vivo in a specialized environment in which their survival and function are assured, while their biological activity is controlled. Extracellular matrix (ECM), with growth factors stored within it, contributes to this microenvironment. While the expression of some ECM components, among which matricellular proteins, is typical of tissue development and organogenesis [5, 6], a number of cardiac diseases, including myocardial ischemia, are associated with qualitative and quantitative alterations in ECM proteins [7]. Cardiac resident or homing stem cells, as well as cells injected in or applied onto myocardium in cell-populated scaffolds interact with ECM components and respond to the local tissue conditions accordingly [8]. Therefore it seems reasonable that the role of ECM and, even more importantly, the effects of the modifications of its composition ongoing in pathological conditions should be studied and taken into consideration when planning the use of cardiac primitive cell-mediated tissue regeneration.
This study describes the method for the in vitro production of ECM typical of adult human heart in the normal and pathological conditions (ischemic heart disease) and highlights the potential use of cardiac fibroblast-derived ECM for in vitro studies of the interactions between ECM components and cardiac primitive cells responsible for tissue regeneration.
2. Materials and Methods
2.1. Cardiac Tissue Samples
Cardiac tissue samples were obtained from the left atrium of hearts from patients with end-stage heart failure due to ischemic heart disease, undergoing heart transplantation (, mean age years, 7 males, 2 females, mean ejection fraction %). Samples of atrial appendages from normal hearts (, mean age years, 6 males, 3 females) were collected from the donor heart waste fragments, that is, tissue trimmed off from the heart while adjusting atrium size and form at the time of organ transplantation. Specimens were collected without patient identifiers following protocols approved by Monaldi Hospital and in conformity with the principles outlined in the Declaration of Helsinki.
2.2. Isolation of Fibroblasts and Cardiac Primitive Cells
Cardiac tissue samples were dissected, minced, and enzymatically disaggregated by incubation in 0.25% trypsin (Sigma-Aldrich, St. Louis, MO, USA) and 0.1% (w/v) collagenase II (both from Sigma-Aldrich, St. Louis, MO, USA) for 30 minutes at 37°C. The digestion was stopped by adding double volume of HBSS supplemented with 10% FBS. This preparation was further disaggregated by pipetting and tissue debris and cardiomyocytes were removed by sequential centrifugation at 100 g for 2 minutes, passage through 20 μm sieve, and centrifugation at 400 g for 5 minutes.
Obtained cell population was used for isolation of fibroblasts and cardiac primitive cells by immunomagnetic cell sorting based on Miltenyi Biotec (Bergisch Gladbach, Germany) protocol. Fibroblasts were purified by positive selection with antifibroblast MicroBeads and passage through MS columns placed in magnetic field, followed by incubation of the collected negative fraction with anti-human-CD117 MicroBeads and positive selection of CD117-positive cardiac primitive cells.
2.3. Extracellular Matrix Deposition and Decellularization In Vitro
The previously published protocols [9, 10] have been modified and optimized for the culture of cardiac primitive cells from adult human heart. Cardiac fibroblasts were seeded on gelatin-coated plates in DMEM supplemented with 10% fetal bovine serum and cultured in confluent state (15 × 103 cells per cm2) for up to 21 days, allowing for extracellular matrix (ECM) deposition. Then, fibroblasts were removed by incubation with a solution of 0.25% Triton X-100 and 10 mM NH4OH in PBS prewarmed to 37°C. The decellularization process was observed at an inverted phase contrast microscope (Olympus Italia, Segrate, Italy). After 1-2 minutes, when cells were no longer discernible, the decellularization solution was diluted and removed, followed by gentle washing of culture plates with PBS. Fibroblast-derived ECM was photographed with a computer-assisted digital camera connected to the microscope (Color View IIIu, Soft Imaging System, Muenster, Germany) and stored at +4°C.
2.3.1. Immunofluorescence
Both fibroblasts during ECM secretion and fibroblast-derived ECM after decellularization (biomatrix) were fixed in 4% paraformaldehyde for 20 minutes at room temperature. After blocking with 10% donkey serum, plates were incubated with primary antibody against fibronectin (rabbit polyclonal anti-human, Sigma-Aldrich), collagen IV (mouse monoclonal anti-human, Sigma-Aldrich), tenascin-C (rabbit polyclonal anti-human, Santa Cruz Biotechnology, Dallas, TX, USA), or laminin (mouse monoclonal anti-human, Sigma-Aldrich) and specific secondary antibodies conjugated with fluorescein or rhodamine (Jackson ImmunoResearch Europe, Newmarket, UK); F-actin was stained with rhodamine phalloidin (Sigma-Aldrich). Nuclei were counterstained with DAPI (Merck Millipore, Billerica, MA, USA), and the stained area of culture dish was mounted in Vectashield (Vector Labs, Burlingame, CA, USA). Microscopic analysis was performed with a Leica DMLB microscope equipped with epifluorescence EL6000 system (Leica Microsystems). Pictures were taken with digital camera connected to the microscope (Leica DFC345FX) and then merged with the software Leica Application Suite 3.6.
2.3.2. Electrophoresis and Immunoblotting
Control plates were used to evaluate the number of fibroblasts when cultured in confluent state: no statistically significant difference in cell number was observed between plates with normal and pathological fibroblasts. Protein extracts were prepared from biomatrix secreted by normal (Bm-N) and pathological (Bm-P) heart-derived fibroblasts. Lysates containing 30 μg of proteins were size fractionated by electrophoresis on 8% SDS-polyacrylamide gel and transferred onto a PVDF membrane (Merck Millipore). Molecular weight markers were loaded onto each gel as a weight indicator. The membranes were blocked and then incubated with one of the following antibodies: tenascin-X, laminin 1, laminin 2, fibronectin, and collagen I, followed by horseradish peroxidase-labelled secondary IgG (all from Santa Cruz Biotechnology). Antibody binding was visualized by chemiluminescence (Amersham Biosciences, Piscataway, NJ, USA) and autoradiography (Eastman Kodak Company, Rochester, NY, USA).
2.4. Cardiac Primitive Cell Culture
Pathological heart-derived CD117-positive cells were plated on biomatrix-covered culture dishes at a density of 8.5 × 103 cells per cm2 in Dulbecco's modified Eagle's medium-Ham's F-12 medium (Sigma-Aldrich) supplemented with 10% fetal calf serum (Sigma-Aldrich), basic fibroblast growth factor (Peprotech, Rocky Hill, NJ, USA), glutathione (Sigma-Aldrich), penicillin, and streptomycin (Life Technologies, Paisley, UK). For evaluation of their proliferation, apoptosis, and migration, cells cultured on surface treated with bovine serum albumin (CTR), biomatrix secreted by normal (Bm-N), and pathological (Bm-P) heart-derived fibroblasts were starved in serum-free medium for 24 hours. All in vitro experiments were repeated a minimum of three times, in triplicate.
2.4.1. Proliferation
For evaluation of proliferation, quiescent cells were incubated with the complete medium for 24 hours, and 5-bromo-2′-deoxyuridine (BrdU) was added (1 : 1.000) for 1 hour before cell fixation. Incorporation of BrdU was evaluated by immunofluorescence with the use of BrdU Labeling and Detection Kit (Roche Diagnostics, Basel, Switzerland), according to the manufacturer's protocol. Nuclei were counterstained with DAPI (Merck Millipore), and the stained area of culture dish was mounted in Vectashield (Vector Labs). Microscopic analysis was performed with a Leica DMLB microscope equipped with epifluorescence EL6000 system (Leica Microsystems).
2.4.2. Apoptosis
For evaluation of apoptosis, cells were incubated with 200 μM hydrogen peroxide in the complete medium for 24 hours and fixed in 1% paraformaldehyde. The fragmentation of DNA was detected using the ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit (Merck Millipore) based on terminal transferase dUTP nick end labeling, according to the manufacturer's protocol. Nuclei were counterstained with DAPI (Merck Millipore), and the stained area of culture dish was mounted in Vectashield (Vector Labs). Microscopic analysis was performed with a Leica DMLB microscope equipped with epifluorescence EL6000 system (Leica Microsystems).
2.4.3. Migration
For the evaluation of migration of cardiac primitive cells in the presence of biomatrix, cells were grown to confluence, and a thin scratch was introduced on culture plates with a pipette tip, producing a cell-free zone [11]. The plates were observed at an inverted phase contrast microscope (Olympus Italia) and photographed with computer-assisted digital camera (Soft Imaging System) at five distinct points, previously marked along the scratch on every culture plate, at various time points. Cell migration was quantified by measuring the width of the cell-free zone (distance between the edges of the scratched cell monolayer) at every time point with Imaging Software for Life Sciences Microscopy (Soft Imaging System).
2.5. Statistical Analysis
All numerical data are presented as mean ± SEM. Statistical differences between groups were evaluated with Student's two-tailed unpaired -test; was considered significant.
3. Results and Discussion
3.1. Biomatrix Production and Characterization
Fibroblasts isolated from samples of adult human heart were cultured in confluent state allowing for ECM deposition in vitro (Figure 1). Incubation with basic solution of Triton X-100 induced membrane permeabilization and cell lysis, resulting in the removal of fibroblasts. Decellularized matrix adhered to culture plate; its presence was observed at phase contrast microscope (Figure 2(a)), while its composition was revealed by indirect immunofluorescent staining of representative extracellular matrix proteins and glycoproteins (Figure 2(b)): fibronectin, collagen, laminin, and tenascin.
An organized ECM is necessary for the arrangement of cells and thus for the maintenance of structure in any given tissue; this intricate interlocking mesh of fibrillar and nonfibrillar proteins and glycosaminoglycans also determines tissue biomechanical properties. Numerous cell types are able to synthesise and secrete ECM components, and their activity is regulated and changes in response to various stimuli, such as inflammation, biomechanical stress, and tumorigenesis [12]. Similarly, already deposited ECM proteins are targeted by specific enzymes, metalloproteinases, that are responsible for the continuous remodeling of ECM and allow cell movement and size adaptation. In any necrotic tissue, fibronectin and collagen deposition is responsible for scar formation, which preserves wall integrity and thickness. Life-threatening pathologies ensue when ECM remodelling becomes exacerbated by chronic stimuli. Also in the ischemic heart disease or cardiac pressure overload, cardiomyocyte hypertrophy is accompanied by interstitial fibrosis, while the necrotic tissue is substituted by a scar [13]. However, in the chronic conditions such ECM remodelling increases wall stiffness, contributes to cardiomyocyte slippage, and worsens the contractile properties of the myocardium [14]. On these bases, it is becoming increasingly evident that secretory activity of fibroblasts, which exceed in number any other cell type in the myocardium, influences myocardial function: it is determinant in normal, but detrimental in pathological conditions.
The synthetic and secretory activity of cardiac fibroblasts isolated from normal or pathological adult human heart can be studied in our model of fibroblast cell culture in vitro. The soluble factors condition the medium used for fibroblast maintenance, while the fibrillar and nonfibrillar proteins are deposited as biomatrix on culture plates. Both ECM components can be further analyzed by analytical methods, and their biological function can be tested on various cell types cultured in the presence of fibroblast-conditioned medium or on decellularized biomatrix. The choice of decellularization method is essential for ECM preservation [15] and subsequent use of extracellular matrix as a substrate for cell biology studies in vitro. Similarity in the composition and biological properties of the decellularized substrates to those of the native tissue is a prerequisite, as it grants the preserved role in the remodelling of tissue structure and modulation of cell function. In fact, many protein components of extracellular matrix react with each other and with the specific membrane receptors based on their secondary, tertiary, or quaternary structure. In our study, decellularization with nondenaturing solution allowed the preservation of protein structure. Further removal of cellular debris was accomplished by gentle washing. If molecular studies of cells cultured on naturally derived matrix are programmed, biomatrix should be treated with endonuclease before cell seeding.
3.2. Cardiac Primitive Cell Culture on Biomatrix
Recently, ECM proteins with a role that goes beyond the structural and mechanical support have been distinguished among other ECM components and termed matricellular proteins [16]. This group encompasses proteins that modulate cell function by interacting directly with cells or by modulating the activity of soluble factors present in extracellular microenvironment, thus influencing cell migration, proliferation, and differentiation [17]. Moreover, these proteins present unique expression pattern, with high levels during organogenesis, virtual absence in normal adult tissue, and reexpression in response to injury and tissue regeneration. In the heart, osteopontin, osteonectin, thrombospondins, tenascin, and CCN family are the matricellular proteins identified so far [18], but laminin-1 also fulfills the requirements [19]. Given these properties, extracellular matrix, and matricellular proteins in particular, can drive cardiac tissue regeneration, described in adult human heart in infarction or pressure overload [20]. Such regeneration depends on cardiac stem cells and their progenies-cardiac primitive cells committed to cardiomyocyte, endothelial, and smooth muscle cell lineage [21].
The biomatrix, obtained in our study by culturing fibroblasts derived from adult human heart, contained various extracellular components, including laminin and tenascin protein family. Consequently, we used this fibroblast derived-ECM as a substrate for the culture of cardiac primitive cells in vitro. Given the differences in biomatrix composition, revealed by western blotting (Figure 2), two types of biomatrix—Bm-N, produced by fibroblasts isolated from the fragments of adult human normal heart of donors died for reasons other than cardiovascular disease, and Bm-P, produced by fibroblasts isolated from the fragments of adult human hearts of patients with end-stage heart failure due to ischemic cardiopathy, undergoing heart transplantation—were used in this part of the study, enabling us to test the integrity and functionality of biomatrix and to compare the effects of ECM typical of normal and pathological conditions on cardiac primitive cell proliferation, apoptosis, and migration in vitro.
Proliferation of cardiac primitive cells (Figure 3(a)) on Bm-P was 147% of control (, ) and 177% of that in the presence of Bm-N (, ). The presence of biomatrix protected cells from apoptosis (Figure 3(b)) provoked by oxidative stress (, ), although no statistically significant advantage of specific biomatrix type was evident. Migration of cardiac primitive cells (Figure 3(c)) was the fastest on Bm-N; in the presence of Bm-P, it was similar to that of control but significantly slower when compared with the speed of migration on Bm-N (, ).
It is arguable that the differences in cardiac primitive cell biological properties observed in the presence of different culture substrates (ECM deposited by normal and pathological heart-derived fibroblasts) reflect the changes in fibroblast synthesis and secretory activity, and thus in biomatrix composition. Several authors, including our group, have described the changes of cardiac stem/primitive cells biological properties in pathological conditions or aging [22, 23], but the contribution of microenvironment has not been considered and sufficiently acknowledged due to the lack of an appropriate model for cell-matrix interaction studies. The molecular constituents of ECM play major role in the responses of cells to their local microenvironment. Both direct stimulation of the specific receptors by growth factors stored in ECM and indirect activation by specific integrin expression and clustering can transmit extracellular biochemical inputs along the intracellular signaling pathways that regulate cell proliferation, survival, and migration [24]. As a matter of fact, the expression of integrin subunits in the same cardiac primitive cell population changes in qualitative and quantitative manner depending on our culture substrate (Bm-N or Bm-P, data not shown). From the above observations it follows that the composition of ECM must be taken into consideration when planning cardiac regeneration based on stem/primitive cell transplantation. Tissue regeneration can be accomplished by cells able to survive, proliferate, migrate, and differentiate in the host environment. While the methods of genetic engineering of stem cells and their application in human disease therapy are still under investigation [25], the aforementioned cell characteristics can be enhanced by cell-matrix interactions.
In mammals, nearly 300 proteins (among which collagen subunits, proteoglycans, and glycoproteins) have been identified as components of ECM [26]. So far, only few of them have been recombinantly expressed or purified and are available for the in vitro studies of their role in cell biology. Considering the interactions among ECM constituents, the possible influence on cardiac primitive cells is difficult to predict. Hence, our model of ECM production by cardiac fibroblasts and its use as a substrate for cardiac primitive cell culture may fill this gap and improve the results of cell transplantation. In the light of our ongoing study, the modification of biomaterials with bioactive molecules [27, 28], such as a native long chain proteins or short peptide sequences derived from intact ECM proteins, that can incur specific interactions with cell receptors should benefit from the description of biological characteristics of cells in the presence of naturally derived cardiac ECM.
In summary, this study highlights the potential use of cardiac fibroblast-derived ECM for in vitro studies of the interactions between components of ECM and cardiac primitive cells responsible for tissue regeneration. In the era of tissue engineering, the choice of biomimetic materials and natural biological components should be based on their specific role on cardiac cell biology. Ensuing knowledge would deepen our understanding of the biochemical cues that regulate cardiac primitive cell survival, proliferation, migration, and, possibly, differentiation, and, hence, guide cardiac tissue regeneration.
Conflict of Interests
The authors declare that they have no conflict of interests.
Authors' Contribution
Clotilde Castaldo, Franca Di Meglio, and Daria Nurzynska contributed equally to this work.
This work was supported by the Future in Research (Futuro in Ricerca) Programme of the Italian Ministry of Education, University and Research (Ministero dell'Istruzione, dell'Università e della Ricerca), Grant RBFR10L0GK (University of Naples "Federico II").
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text:
Standard Cutting Service
Premium Cutting Service
Economy Cutting Service
Fancy Color Cutting
24-Hour rush service when available
Upon Request we can work with a laboratory of your choice to save time and money
Small Stone Repair Service and Melee Repair
Rough Cutting
Shipping Charges
All merchandise is fully insured whilst on our premise for your stated value against loss or theft. While every care is taken whilst working on your diamond, the process of diamond cutting does have a small risk of breakage or damage during the cutting process. Southwest Diamond Cutters will assume no liability for damage incurred during the cutting process.


text:
Last night I went over to my family's house, and my cousin made this dessert I've never had before. When she said it includes cream cheese and whipped cream -- I was sold!
It's an easy recipe, and I would be a very, very selfish person if I didn't share the recipe with all of you.
All that I ask if that you will promise to send me some. But if you don't, I understand. I won't harbor any resentment or anything. (>_>)
Anyway, it's called ambrosia. It's sort of like a fruit salad, but it's totally not healthy. If yogurt became amazing, it would taste like ambrosia.
What You'll Need:
16 oz. of cream cheese
12 oz. of Cool Whip (from tub, not can)
14 oz of condensed milk
Your choice of fruits
How To Make It:
Mix well
Refrigerate and cool for about 1 hour
What To Do Next
Close your eyes and take a bite. Imagine this is what heaven must taste it.


text:
We don't see churches as clients. We see churches as partners. Our role is to come alongside churches and help them carry out their mission by creating logos, websites, print media, custom artwork, etc that communicate the message they have been given to speak.
Our goal is that you get solutions that fit your specific situation, and we spend time on the phone or over coffee to make sure we understand what you are looking for. We communicate regularly during the project, so you are in the loop from beginning to end.
Every church is different, and we treat them that way. No templates or stock layouts here. Instead, we build a custom solutions so that you get what you need without paying for a lot of extras that you don't. Also, because of our experience, we work fast, so even though our projects are custom, we can turn them out quickly and still be affordable.
Different styles communicate different things, and Studio Nine Creative is all about creatively expressing what ever message you need to communicate. Just check out our portfolio, and you'll see a wide variety of styles and approaches, each creatively generated for a specific need.
We know church budgets can be tight. That is why we strive to work within your budget, building custom solutions that fit your needs in an affordable way. Give us a call today, and we can talk about your project, your budget, and find your solution.
Phone: 405.820.7134 | Email: email@example.com


text:
ALEXANDRIA, Egypt (Reuters) - Police fired tear gas to disperse hundreds of demonstrators gathered on Tuesday at Alexandria University to protest against mass trials of Muslim Brotherhood members, a Reuters witness said.
The leader of the Brotherhood and 682 others were put on trial earlier in the day in the southern town of Minya on charges including murder, a day after more than 500 other supporters of deposed Islamist president Mohamed Mursi were sentenced to death.
(Editing by Michael Georgy and Andrew Heavens)


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Christina Aguilera, 'Your Body': Track Review
"Your Body" (4:00)
Producers: Max Martin, Shellback
Writers: M. Martin, S. Kotecha, Shellback, T. Amber
Publishers: various
Although Christina Aguilera released music throughout 2010 (the underperforming Bionic trotted out three singles, and she contributed new songs to the "Burlesque" soundtrack), it's tempting to call "Your Body" her long-awaited comeback. Given that it's her first collaboration with mega-producer Max Martin, it's clear the diva is going for broke. Featuring a chorus that allows Aguilera to unleash her giant pipes, "Your Body" makes it almost easy to look past its wince-worthy lyrics about anonymous sex ("So don't even tell me your name/All I need to know is whose place," goes one couplet). Indeed, the song was shipped to radio with a kid-friendly chorus, "All I wanna do is love your body," while an explicit version swaps out "love" for something far more transactional. With new album Lotus arriving Nov. 13, Aguilera, love her or hate her, has earned the right to belt "I think you already know my name" on the song's bridge.-AH


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Fibers and fabrics with insulating, water-proofing, and flame-resistant properties
United States Patent
|April 20, 2004
|View the Complete Patent at the US Patent & Trademark Office
|Lawrence Livermore National Laboratory - Visit the Industrial Partnerships Office Website
|Fibers, and fabrics produced from the fibers, are made water repellent, fire-retardant and/or thermally insulating by filling void spaces in the fibers and/or fabrics with a powdered material. When the powder is sufficiently finely divided, it clings tenaciously to the fabric's fibers and to itself, resisting the tendency to be removed from the fabric.
|Hrubesh; Lawrence W. (Pleasanton, CA), Poco; John F. (Livermore, CA), Coronado; Paul R. (Livermore, CA)
|The Regents of the University of California (Oakland, CA)
|10/ 002,513
|October 25, 2001
|The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the United States Department of Energy and the University of California for the operation of Lawrence Livermore National Laboratory.


text:
Mouse Extender 22.214.171.124
Mouse Extender 184.108.40.206 MouseExtender - Launcher For W...
This program allows you to improve your productivity. It is extremely simple and fully customizable.
MouseExtender Features
1. Mouse button configuration
2. Add items to ME using Drag& Drop!
3. Group your items by tabs
4. Add Web Address
5. Add Item From Currently Running Programs
6. Customize item as you want. User can change launch arguments, tool-tips, icons, etc.
7. Shutdown/sleep/hibernate with timer
8. Automatic updates checking
9. Sort Items using Drag& Drop
10. Stylized controls
11. Enable/Disable option from tray icon context menu
12. About information
13. Localization
14. MouseExtender automatically adds web site icon and title for Web address
15. Internet proxy connection configuration
What is new in official Mouse Extender 184.108.40.206 software version? - Removed extra PreEmptive features requests Increased startup performance Russian localization updated. What is expected in the future? Newly-made Mouse Extender 1.10 be downloaded from current page, we also looking forward to unconfirmed 1.11 release build. You may download 129191@releaseId=30128 directly, estimated download time by ISDN or CDMA [~128 kbit/s] is 0:00:16. Just write the reviews of the Mouse Extender. System requirements are CPU: 1.6 Ghz, Graphics: 64 MB of video memory, HDD: 1 MB, RAM: 50 MB. Program has been scanned and verified by the several antivirus and anti-spyware applications and Mouse Extender found to be clean. New guides and Mouse Extender tutorials are easy to follow and give you the knowledge to use Mouse Extender 126.96.36.199 software. The following languages are supported by Mouse Extender: English.


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Pope Descends on USA. What's His Mission?
& anti-gay agenda. He'll be entertained & greeted by American
leaders, Catholic elite & parishioners in Washington & New York.
Of course Boston was excluded from his itinerary. Perhaps this is
because we had a nasty sex scandal with the Catholic Church.
Or could it be the fact that Massachusetts is the only state in this
country where gay marriage is the law?.....:)
In the name of religion, he will sermonize his disapproval of a
woman's right to choose, birth control & stem cell research.....
Then there is his continual attack on our LGBT community.
All we want are the same equal rights & protections for our
families that heterosexual citizens have. It's sad to think that
the Pope believes that we deserve less. For more info for our
LGBT Catholic Community.... Updates to come....
Washington Blade: Pope Waves to Gay Catholic Group
View Our Trailer on Gay Marriage


text: By. Margcata. The Julia Child of Mexico, Bilingual Susanna Palazuelos, Chef, Caterer ( S.P. Catering in Acapulco, Mexico ) and Authoress, has recently released her latest legacy on Mexican Gastronomy. This is a must for aficionados ( enthusiasts ) of the uncountable regional cuisines of Mexico. The photos are absolutely wonderful and the guidelines on how to´s --- are as if Susanna were speaking to you face to face providing all the guidance you need. Her catered dishes have delighted members of Royalty, Prime Ministers and Epicurean Events worldwide. This edition is now available in English in the U.S.A. I have tried her Mango Prawns which were to die for. What has impressed me most, is that I had lived in Mexico D.F. for two years back in the 1970s and Susanna´s Mexican cuisine is light ... piquant without being unedible and wonderfully interesting as I remember it ... I am off to have some fun with it on Saturday for lunch ... Happy Holidays, Margaux.


text:
Evelyn Lozada Metallic Clutch
Evelyn paired this all white outfit with a gold metallic clutch and shiny shoes.
Evelyn Lozada Peep Toe Pumps
These embellished peep toe purple pumps add some flare to Evelyn's look.
Browse: Evelyn Lozada | Metallic Clutch | Peep Toe Pumps
Popular Lookbooks see all looks »


text: This LED from Ronhill is designed to help keep your safe whilst out on a morning or evening run. In hard to see conditions, this LED can be set to glow or flash illuminating yourself to passers by and drivers. The item is flexible shock resistant, easy to use and lightweight. When charged the battery can last up 150 hours.


text:
The organisations in the New Forest Advice Network all provide free, confidential advice on a wide range of issues affecting people of all ages living in the New Forest. These range from advice on welfare benefits, debt and money management to help with employment, housing or health and community care.
See Web Site for more detailed information about the New Forest Advice Network
Use the Directory Search Facility to find you identify the sources available to you.
Free, confidential advice from a variety of sources on a wide range of issues affecting people of all ages living in the New Forest. These range from advice on welfare benefits, debt and money management to help with employment, housing or health and community care.
Citizens Advice is a local independent charity reliant on committed volunteers and local funding; it provides free, impartial and confidential advice and information to all in the community who need it; and it campaigns on issues which impact adversely on people's lives.
39 - 41 Old Milton Road. New Milton. BH25 6DJ
Monday - Thursday 10:00 a.m.until 2:00 p.m.
01425 628178
03444 111 306
New Forest CAB Web Site
The Arnewood Practice. Avenue Road. New Milton. BH25 5JP
Phone for Appointment
Gabrielle Field Bsc. Msc. MBACP
07810 457675
One to one counselling, couple counselling and group therapy
Ashley Baptist Church. Lower Ashley Road. Ashley. BH25 5AA
Meets 3rd Tuesday of the month at 7:30 p.m. until 9:00 p.m.
01425 623443
Avenue Road. New Milton. BH25 5JP
By Appointment
Initial Consultation free of charge
Roger Andrew DipHyp Dip HE CQSW
01425 620096 / 07837 326250
NFDIS. 6 Osborne Road. New Milton. BH25 6AD
Most normal shopping hours
01425 628750 - Helpline
01425 623485 - Administration
NFDIS also runs a Monthly Lottery with Tickets only costing £1.00. Cash Prizes for players over 16. Call into the Shop or telephone for more information.
5 Whitefield Road. New Milton. BH25 6DE
Call for Appointment
Free to all
01425 620780
Bridging the gap between you and drug and alcohol treatment services.
Who is the Bridge For :-
Adults 18 years and over with drug or alcohol issues and for Adults vulnerable to relapse. We can also help Parents, Partners and Carers of Bridge Project clients.


text:
Credit Cards to Help Build or Rebuild Credit – Credit Cards for Recovering from Bad Credit
Developing good credit it is important. Most people start when they are younger so that they learn how important it is to have the credit that is necessary to buy items. They might take out a car loan so that they can show a bank how they can work and pay the amount back on time.
This will be reported to the three major credit bureaus – Transunion, Equifax, and Experian. Keeping a good credit score is very important. Many people also take out credit cards to help them get the credit that they need. When you have bad credit, you will want to rebuild credit. There are many people that have bad credit from going through tough times. It usually occurs from not paying the bills on time, and it is on your credit report. Recovering from bad credit doesn't always have to take a long time. Bad credit can be removed by bargaining with creditors.
Credit Cards are Great When They are Used Right
There are many people that want to have access to credit cards so that they can pay back what they owe on a timely basis. Credit cards allow people to purchase without paying the full amount first. They are also good for helping people to build credit and also rebuild credit. There are many different credit cards to pick from. If you are looking for credit cards to build credit or rebuild credit, you should complete a search online to find the different credits cards companies that can give you the credit cards. Print the information out so that you can find the one that will work the best for you.
Recovering from Bad Credit
If you have credit that is bad, remember what got you into the situation in the first place. You might not have made enough money to pay your bills, or you did not have a good budget in place. Other people have gone through a loss of a job or a divorce that has caused them to get bad marks on their credit report. You want to keep the bad marks from coming back so you will want to pay more than the minimum amount on the credit cards that you get. You can do this if you are determined and disciplined.
There are Credit Counselors that can Help to Rebuild Credit
Recovering from bad credit may seem like an insurmountable task for some people. If you are one of these people, you might want to enlist the help of a credit counselor. These credit counselors are adept and knowledgeable. They can sit with you and discuss why you have bad credit. They can also help you to correct it. When you are discussing your situation with them, don't be afraid to ask the questions that you have because they will give you the answers that will matter.
Other People can Help You to Build Credit Again
Credit cards can help you in recovering from credit that is bad, build credit or rebuild credit. It is always a good idea to talk your situation over with someone that knows a lot about your situation and how to help you to keep good credit. It can make a huge difference when someone else sees it with their own eyes, and they can help you to plan a budget that will work. You should also be careful in how you use the credit cards so that you are not spending too much on things that you might not need.
People that learn how to take care of their credit will generally not have another problem again. Instead, they will build credit. They will take care to make sure that they watch what they are doing with their finances at all times. If you still experience problems after correcting the issues that plagued you in the first place, it might be a good idea to let another person handle your finances for a time being until you are ready to do so yourself.


text:
cars car seat1,328 Products found
Portable Gel Car Seat/ Car Seat
Min.Order: 1000 Piece/Pieces
34 cooling vents to help maintain firmness.
Includes built in handle for portability.
Gel and neoprene.
Hammock Style Waterproof Pet Car Seat Cover
Min.Order: 1000 Set/Sets
Pet Car Seat Cover
heavy duty nylon fabric with PU coating on the back for water proof
easy to install and comes with instruc
Pet Car Sea
Toilet seat cover 017
Toliet seat cover 017
road sharp,soft close
outside size 425*350mm
inside size 275*205mm
made by PP
Toilet seat cover 012
Toliet seat cover 012
square sharp,soft close
outside size 475*370mm
inside size 320*215mm
made by PP
Toilet seat cover 011
Toliet seat cover 011
square sharp,soft close
outside size 425*350mm
inside size 295*215mm
made by PP
Toilet seat cover 010
Toliet seat cover 010
long road sharp,soft close
outside size 440*355mm
inside size 310*220mm
made by PP
Toilet seat cover 009
Toliet seat cover 009
long road sharp,soft close
outside size 450*360mm
inside size 300*220mm
made by PP
Toilet seat cover 007
Toliet seat cover 007
road sharp,soft close
outside size 445*385mm
inside size 295*230mm
made by PP
Toilet seat cover 006
Toliet seat cover 006
road sharp,soft close
outside size 450*370mm
inside size 300*220mm
made by PP
Toilet seat cover 002
Toliet seat cover 002
road sharp,soft close
outside size 470*365mm
inside size 295*210mm
made by PP
Computer rack car third generation car desk
Min.Order: 3000 Set/Sets
18pcs/ carton
Ctn :59*54*38
Toilet seat cover 057
Toilet seat cover 057
long round sharp,soft close
outside size 445*385mm
inside size 300*200mm
made by PP
car seat
Custom hawaiian auto seat covers are available for most foreign and domestic cars, trucks. To be mentioned is that the car and truck covers we offer i
1.For baby between 15-36kgs
2.5 point harness
3.Standard crash test
4.IECE certificate
[Manufacturer, Trading Company]
Min.Order: 450 Piece/Pieces
Forward Facing
For Baby between 9-36KG (9months-12 years old) Group I II III
Ucomfy Portable Gel Car Seat/ Car Seat /GEL SEAT
Min.Order: 2000 Piece/Pieces
Enjoy lasting cushioned comfort with the UComfy. Combines gel and foam for an optimal custom conformed balance of firmness and comfort in the office
[Manufacturer, Agent]
Child car seat belts
Min.Order: 500 Piece/Pieces
100% polyester
5 point baby seat belt
For baby/children
With ISOFIX
CCC and ECE R16 certificate
[Manufacturer, Trading Company]
Pvc Leather For Car Seat
Min.Order: 1000 Meter
1) Thickness:1.1mm
2) Width: 140cm
3) Packing: 40m/roll.
4) No Hole, No Dirty On The Pvc Face.
5) Non-woven Fabric As Backing.
[Trading Company]
baby car seat, child car seat
Min.Order: 100 Piece/Pieces
1. baby car seat
2. ECE R44/04 certificate
3. For Group: 0+1
4. For children 0-18kgs, 0~4years
5. 5-point harness with one pull adjustment
[Manufacturer, Distributor/Wholesaler]
baby car seat
ECE R 44/04 baby car seat/convertible seat
Group I, II, III
Fits for 9kg to 36kg (app. 9months-12years children)
Car swivel seat cushion
Min.Order: 1000 Piece/Pieces
1) Swivel cushion
2) Material: Slow rebound sponge
3) Diameter:40cm
4) OEM/ODM is OK
[Manufacturer, Trading Company]
Car Seat Gas Spring
Application in car seat
Easy installation
Make the seat front and back freedom
OEM offered
QPQ piston rod
Baby Car Seat
Min.Order: 200 Piece/Pieces
Baby car seat With ECE R44/04
Group I,II,III
Convertible Seat(9-36kg)
Net Weight:4.8kg
Produce Size:46*46*71cm...
[Manufacturer, Distributor/Wholesaler]
Baby Car Seat
Min.Order: 500 sets
Price:US$550.0;Productivity:4pcs/carton;Min. Order:500 sets;Packing:995x495x690mm;DeliveryDate:within 20 days;PaymentType:T/T , L/C;Trademark:kissing
Min.Order: 1000 Meter
Material: 100% air-texture polyester yarn
Design:plain twill fabric
Weight: 230gsm
Width: 150-180cm
Cooling car seat cushion
Min.Order: 1000 Piece/Pieces
Cooling Gel Insert
Deluxe Memory Foam
Portable Design
All season comfort
[Manufacturer, Trading Company]
Sheepskin Car Seat Cover
Min.Order: 200 Piece/Pieces
Car Seat Cover
Sheepskin as material
Low wool 2.5-3cm,High wool 5-8cm
Good price at USD 25-35 one pc
Colors are customized
[Manufacturer, Trading Company]
Productivity:10000PCS/MONTH;Min. Order:10 PCS;Packing:BOX;Transportation:BY SEA,BY AIR;DeliveryDate:30DAYS;PaymentType:TT,L/C;Trademark:SEINECA;Funct
Car Seat Cover
Productivity:20000/months;Min. Order:500 sets;Packing:in the zipper bag;Transportation:by shipper;DeliveryDate:25-35 afer reveived deposite;PaymentTy
[Manufacturer, Distributor/Wholesaler]
Car Seat Cushion
Productivity:10000000 Sets per Year;Min. Order:1000 Set;Packing:carton;Transportation:by sea;DeliveryDate:20 days;PaymentType:T/T OR L/C;Trademark:OE
[Manufacturer, Other]
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Main Export Markets
- North America (1141) Western Europe (1132) Eastern Europe (1081) Eastern Asia (1078) Southeast Asia (1077) South America (1061) Africa (1047) Mid East (1009) Oceania (956)
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