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They still have nine games on their schedule, but as far as the Hamilton Bulldogs are concerned, the playoffs have already begun.
The team played one of its speediest, most hard-hitting games of the season in Kingston Sunday — a preview of a potential first round matchup. And, while it wasn't the ending they wanted, head coach John Gruden was happy with his side's effort in the 3-1 loss.
"We competed," he said. "Our guys never quit, we had lots of good chances and that's the type of feel it's going to be. We have to realize that and understand that and do better."
The Bulldogs went into Kingston one point ahead of the fifth-place Frontenacs in the Eastern Conference. Now, they're one point behind — in the standings and the fight for home ice advantage.
Gruden, however, isn't fazed.
"I don't think it matters," he said. "Playoff hockey is playoff hockey."
His players don't necessarily agree.
"We want to have home ice," said Ben Gleason.
"It's huge," added Reilly Webb, who returned to the lineup after a nearly yearlong absence Thursday. "We're pushing for that right now."
On a weekend in which the Bulldogs expended a lot of energy for a solitary point (they also lost 5-4 in overtime in Ottawa), Webb's play on the blueline was one of the bright spots. In spite of missing most of the last two seasons with a shoulder injury, the 17-year-old from Stoney Creek was plus-5 in his first three outings and wasn't on the ice for any of the opposition's 11 goals. Against the 67's, Gruden even opted to put him in to kill a penalty with less than four minutes to play in regulation and the score tied.
"He just simplifies things," he said. "He does a lot of the little details, he's got a really good stick and he boxes guys out. He plays the body really well."
Gleason, who asked to play with Webb when his regular partner, Justin Lemcke, was out with the flu Saturday, backed Gruden up.
"He's simple," said the 18-year-old defenceman. "He's good at moving the puck and he loves to make a good move here or there. He's smart and he's quick with his feet. He's got some things to work on, but that's part of being young and not having many games under your belt."
For Webb, meanwhile, the return was nerve wracking. Yet, with each shift, he started to feel — and look — more comfortable.
"Once I got through my first few shifts, I wasn't nervous anymore. Just back to what I'm used to," he said. "It felt good."
On Sunday, Webb's draftmate Matthew Strome scored the lone Bulldogs goal on a wraparound, while Ted Nichol, Linus Nyman and Brett Neumann had the Kingston markers. Dawson Carty turned aside 18 shots in the loss — his first in seven games.
The turning point was likely Nyman's game-winner, which he notched with two Bulldogs in the box and Carty out of position a few minutes into the third. It was the second time in two days penalties cost them. In Ottawa, the Bulldogs were booked for six minor infractions and allowed the 67's to score twice on the power play.
"We lost that part of the game and that was the difference," Gruden said. But it wasn't the only factor.
On top of Lemcke — who played Sunday, but was still under the weather — the Bulldogs were missing Matt Luff (concussion) and Mackenzie Entwistle (mononucleosis) again this weekend. In particular, their absence was felt against the Frontenacs, who allowed few scoring opportunities and got in the way of a ton of Hamilton shots.
The Bulldogs, who are now 3-1-0-1 against the Frontenacs, visit Kingston once more before the end of the regular season. When asked if he'll do anything differently then — or, potentially, in the playoffs — Gruden said no, but he is looking forward to getting Luff and Enwistle back.
"We're going to be with three lines that are going to come extremely hard," he said. "It just comes down to execution and paying the price and special teams. That's what playoff hockey is all about."


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- Home
- Conferences
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Almost every religion in the world begins and ends with us. We do something that makes the God of the universe possibly change. In Christianity God reveals himself in the person of Jesus. Everything begins and ends with Jesus.
If you have questions or topics that you'd like discussed on an HT-Video Short, email them to email@example.com or send a text to 936-647-3235.
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The mission of Higher Things® is to assist parents, pastors, and congregations in cultivating a distinctly Lutheran identity among their youth and young adults.

subset: commoncrawl


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I've always held that there's very little difference between recognition and appreciation. Whether it's something we've evolved (some kind of neurological synesthesia) or perhaps learned (an infant recognizing its mother will surely feel a sense of appreciation), we're all guilty of getting the two mixed up.
For Example
Have you ever, while listening to the radio and a song begins so play, thought "Oh, I love this song," only to realize moments later that in actual fact you hate it? That's what I'm talking about. You got recognition and appreciation mixed up.
This Matters Why?
People use this phenomenon to sell you crap. That's what branding is. You've heard the term "brand recognition" – they're hacking into your brain to tap your appreciation reserves. That's why companies have logos, jingles, official colors (did you know that Cadbury's has a patent on the color purple?*) and all that. Of course, sometimes it's impossible to do this if the product's too awful; you soon begin to subconsciously equate the branding with the poo. Which is why TV companies change their catch-cry every year. To renew the recognition-appreciation bond.
You have yourself to blame.
If you're not aware of this, and you go out and buy a crap album that's on high rotation on [insert commercial radio station here], you're being duped. That's fine – sometimes we don't mind other people doing our thinking for us – but you should be aware of it, all the same.
I have no intention of exploiting your mixed up brain for my benefit. So I have no obligation to imprint you with branding or whatever. So I'll change the theme, interface, even the title of my blog whenever I feel like it. So that's why i have no problem renaming this site from "Distance Over Time" to "Interesting Developments."
* I just made that up. But Toblerone does have a patent on that triangle box of theirs.**
** Probably.


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This is great! I am having so much fun! This is shedding so much light on how I see the world. Thanks!
What strengths do INFPs bring to their work?
At work, the INFP is not particularly driven by money or status, preferring work that aligns with their personal values and allows them to help others. INFPs are typically motivated by vision and inspiration, and want to engage with projects and causes that feel important to them.
INFPs enjoy the process of creative problem-solving, and want to understand complex issues. They appreciate innovation, and want to come up with original ideas to improve circumstances for people. An ideal job for a Healer allows them to express their individuality in the way they work, and takes advantage of their ability to see unique solutions.
INFPs enjoy working autonomously and having control over how and when to complete a project. They often enjoy participating in teams, although they want to be free to put their own personal stamp on their work. When they do work with other people, it's important to the INFP that they be cooperative, supportive, and flexible, and that they have similar passion for their ideals.
INFP career facts
- Second lowest average income of all the types, at $31,508
- Most likely of all types to stay home with their children
- Fourth lowest in job satisfaction out of all the types
- Slightly more likely than average to be self-employed
What are some good careers for an INFP?
Top careers for the INFP include:
Arts and Design
Community and Social Service
Education and Library
- Archivist or Curator
- Elementary School Teacher
- Librarian
- Preschool Teacher
- Professor or College Instructor
- Special Education Teacher
Health Care
- Audiologist
- Chiropractor
- Nutritionist
- Genetic Counselor
- Massage Therapist
- Midwife
- Occupational Therapist
- Physical Therapist
- Speech-Language Pathologist
- Veterinarian
- Veterinary Technician
Business and Management
- Anthropologist or Archaeologist
- Geographer
- Historian
- Psychologist
- Sociologist
- Zoologist or Wildlife Biologist
How can an INFP find the right career?
INFPs, like all personality types, are most satisfied and successful when they choose a career that takes advantage of their natural strengths, talents, and interests. If you're searching for the right career, check out the Career Personality Profiler test, which provides a complete assessment of your personality, interests, and aptitude.
What careers should the INFP avoid?
It is important to note that any personality type can be successful in any occupation. However, some occupations are well suited to the natural talents and preferred work style of the INFP, while other occupations demand modes of thinking and behavior that do not come as naturally to this type. Occupations that require the INFP to operate outside their natural preferences may prove stressful or draining, and often sound unappealing to INFPs who are choosing a career.
The following occupations have been found to be unpopular among INFPs, based on data gathered from surveys of the general population.


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1. Teach them how to make Planet Earth a better place to live: the children are the future of the nation. The earth resources are fast depleting and it is the children who will be going to use them in future. So teach them to start saving on from today and work towards creating a sustainable environment.
All these activities undoubtedly will help them become a responsible and a better person in life and this is what every parent wishes for.
Visit Also:


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Migne, Jacques Paul
Migne, Jacques Paul zhäk pôl mē´nyə [key], 1800–1875, French publisher of theological works, a Roman Catholic priest (ordained 1824). He set up a printing press in Paris and printed many religious and theological works. His principal publication was called Patrologia, an ambitious project the aim of which was to publish the writings of all Christian writers into the Middle Ages. The texts reproduced in the Patrologia series are being replaced only gradually with better editions. There were three series: Latin Fathers to Innocent III (217 vol. and an index in 4 vol.) Greek Fathers (to 1438) in Latin translation (81 vol.) and Greek Fathers in Greek and Latin (166 vol.).
The Columbia Electronic Encyclopedia, 6th ed. Copyright © 2012, Columbia University Press. All rights reserved.
See more Encyclopedia articles on: Roman Catholic and Orthodox churches: General Biographies
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Oasis 25RK
Shasta Oasis travel trailer 25RK highlights:
- Rear Kitchen
- Private Bedroom
- Single Slide
- Dual Entry/Exit
- LED Lighting
For an impressive home base anywhere you travel, choose this Oasis travel trailer that features a unique rear kitchen layout. The large pantry is convenient for stocking up on meal-time essentials before you head out. You will find the interior of this trailer to be quite spacious because of a single slide that has been included, and the private second entry door in the front bedroom allows you to slip out underneath the LED-lit electric awning for a late night to enjoy the outdoor surroundings.
With any Shasta Oasis travel trailer you can expect quality, comfort, and value in every model. Inside of each Oasis you will enjoy LED lighting, Carefree flooring that is super easy to clean, and handcrafted pocket-bored and screwed cabinetry to ensure years of use. There is lots of storage space throughout, as well as comfortable furnishings, and you can easily cook your favorite camp-out meals with the handy 3-burner cooktop stove and 6 cubic foot refrigerator. When it comes to keeping everyone clean and smelling fresh, the 6 gallon gas DSI water heater allows you to take long hot showers, without any problem at all!Have a question about this floorplan?Contact Us
Sleeps 5 Slides 1 Length 29 ft 9 in Ext Width 8 ft Ext Height 10 ft 9 in Interior Color Chocolate, Platinum Hitch Weight 538 lbs Gross Weight 7538 lbs Dry Weight 5257 lbs Cargo Weight 2281 lbs Fresh Water Capacity 42 gals Grey Water Capacity 72 gals Black Water Capacity 36 gals Furnace BTU 18000 btu Available Beds Queen Refrigerator Size 6 cu ft Cooktop Burners 3 Shower Size 40" Number of Awnings 1 LP Tank Capacity 20 lbs. Water Heater Capacity 6 gal. Water Heater Type Gas DSI AC BTU 13500 btu Cargo Deck Size 0 ft Awning Info 16' Electric with LED Lights Axle Count 2 Number of LP Tanks 2 Shower Type Tub/Shower Combo
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Friends/Contact/Buddy lists?
are able to do this, i don't see it anywhere.
Contact/Buddy list?
Since this is a new forum I'm looking for everyone's feedback so that we can customize the site accordingly. Post your suggestions and ideas here!
Post Reply
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I realise 'discovered' sounds a little pompous, but stick with me. Other vocabulary didn't have the same ring to it.
When I was thinking of brands and products to include in this post, I initially skipped over both Nip & Fab and Origins because it feels like I've been using them for so much longer. It was definitely 2015 that saw these enter my life, though, both beginning with a mask.
Nip & Fab | I was first introduced to Nip & Fab through being sent the Glycolic mask to review. It was the first mask to make a substantial difference to my skin, so I then began to look into more of the range. I now have four of the Glycolic products and like them all, but the pads are probably my favourite because of how quick and easy they are to use. I also own a couple products from the Dragon's Blood range, and while they don't make as big a difference to the surface of my skin, I still like them. Like they say on the packaging, they leave my skin feeling plump and softly moisturised.
Origins | I bought the Mask Marvels set early last year simply because it was an incredibly bargain, and each mask in the set was highly rated. The Out of Trouble and Clear Improvement masks were nice, but it's the Drink Up Intensive one which really impressed me. I then bought the Cleansing Oil, which although good, I still prefer The Body Shop's Camomile version because it's incredibly similar, but half the price.
Just before Christmas, the gift sets were on offer and my discount, for some reason, doubled up, making them a very good price indeed. It would have been slightly cheaper to get them in the half price sale, but when that rolled around, they were no where to be seen from the beginning, so I'm glad I bought them early! I've been liking the GinZing moisturiser from one of these sets. It smells deliciously orange-y, and is refreshingly light. Having combination oily skin means I don't need something heavy duty. Is it any better than cheaper light moisturisers I've come across? I'm not sure. I just know that I like it.
The other set included the KissZing Lip Crayon in Rosemance, which was my main reason for buying it. It has quite a satin finish, which surprised me, and it's not as balmy as I expected, but it wears well and looks beautiful on. I also have a couple of GinZing Brightening Mascara samples, and while I'd never pay £17.50 when I love my £7.99 ones, it's still a good mascara. It doesn't clump or flake, and just makes your lashes look natural. I'd recommend this to someone over the Benefit ones, and they're more expensive.
Zoeva | Zoeva brushes now dominate my collection. The quality of them is so much higher than their price point. My favourite has to be the 322 Brow Line because it's thinner than any other eyebrow brush I've tried, so doesn't pick up too much product, which is great when using pomades. The 102 Silk Finish is also my favourite for foundation. Even after all the battering it's gone through, it still looks great. The 127 Luxe Sheer Cheek is the only one I've been slightly disappointed in, and I think this is because the hairs aren't synthetic. If you'd like to read reviews of every Zoeva brush I own, click here.
Makeup Revolution | I know it's the brand which rips off other brands, but I really like some of the products, and they're cheap as. The Iconic Pro Blush, Bronze, and Brighten Palette in Rave is a little powdery, but apart from that, it's a good all rounder. The bronzer is matte and subtle, the highlighter's of a nice candlelight shade, and the blush has the warmth I tend to gravitate towards. For £4, it's worth giving it a go. The Ultra Brow Arch & Shape is a dupe for Anastasia Beverly Hills and Soap & Glory's offerings, and I'm a fan. The pen end has all but dried up now, but that's because I've had it a while, and it's down to this type of product, but the pencil end is still soft. It really is worth the £3.50. The Ultra Aqua Brow Tint is just £3. I didn't use it a lot in 2015 actually, but I think it's going to be in my next favourites post. A claimed dupe for the Makeup Forever Aqua Brow and NYX Eyebrow Gel, it's pigmented, easy to use, and stays put.
I've only tried the brow products from both Anastasia Beverly Hills and Freedom, so I can't say I 'discovered' the brands, but still wanted to mention them. The Anastasia Beverly Hills Dipbrow Pomade in Soft Brown is as good as everyone says, but mine's starting to dry up, and instead of spending another £15, I'd rather buy Freedom's £5 version, also in Soft Brown. It's slightly warmer, so I've also bought Medium Brown since, but in terms of formula, I don't think it's massively different. The Duo Eyebrow Powder in Soft Brown (£4) is also nice. It's not massively pigmented, so you do have to dip your brush a few times, but it's one to use when you want a natural look, or to set your eyebrows after using another product.
I hope I discover just as many great brands in 2016. What do you think of the ones I've talked about?


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Multiple Google accounts, login with one browser, simultaneously
I am a big fan of various services provided by Google and I do own multiple Google accounts for different purposes. Now that I'm keeping up a blog through Blogger, I find it's a little annoying not being able to retrieve my emails through one account, organize photos and blog through the others all at the same time.
Did a quick search today on Google and spotted various workarounds for it. Yes, workarounds as they weren't really a solution that allows me to manage multiple Google accounts for different services all at the same time with one application. Creating multiple browser profiles or open up different browsers just isn't satisfying. I finally found this Firefox add-on called "CookiePie" that does just what I wanted. Luckily, I happened to be a Firefox user!
What it does is to allow you to maintain different cookies storage through different Firefox tabs or windows at the same time! So, you can login one account under tab 1 and the other under tab 2. This doesn't just work with Google, but also your other accounts from Yahoo!, Windows Live, or AOL. Just when I was going to download and install this baby, it says "Windows" and "Linux" Only…. Again, lack of support for Mac users. (I am one of the victims) Although some claim it works fine on their Macs, but it didn't work for me. Not sure if it's because I am running Firefox 3 as it doesn't state on the website that this extension is Firefox 3 compatible, but no "incompatible" warning after I installed it though. Anyways, look forward to the newer version and the compatibility for the Mac.
For anyone doesn't run a Mac or just wanna give it a try, get a piece of your pie Here


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The Hobbit production suffered an upset over the weekend, Guillermo del Toro announced that he was leaving The Hobbit's directorial seat and the movie is still stagnated on the shelf with the financial woes of MGM. Not only that, there is chance that Jackson will not taking over the job too. Although he publicly announced his intention to direct if he had to, there are some problems around - particularly his binding contracts with other jobs.
In today's feature, Green Tea Movie lists who should replace Guillermo del Toro if Peter Jackson isn't. Ideally, Peter Jackson should but what if he is not? Too bad, there are only three who realistically should take over del Toro's job.
1) Alfonso Cuaron
He's Mexican too but that's not all. He is the director who made Harry Potter and the Prisoner of Azkaban to become the most favourite Harry Potter movie among the fans. Yes, he is a good story-teller and a good film-maker as well. His vision for the third Harry Potter has changed the way how a middling fantasy art can be a dramatic adventure. Besides, he shared the same passion with Guillermo del Toro and they have been friends for the last 20 years. Cuaron produced del Toro's incredible Pan's Labyrinth in 2006 and the two are co-owners of Cha Cha Cha Films with Alejandro Gonzalez Inarritu. Anyone who had watched Children Of Men (Owen Wilson's searching the missing baby was one of the most highlighted scene in the movie) and Y Tu Mama Tambien will know how brilliant this director is.
2) Neill Blomkamp
He is the apprentice of his fellow mentor Peter Jackson. He made a bold attempt of bringing in his brainchild and visionary story in District 9 and it proved to be a hit. District 9 was so loved by many - helm as one of the best science fiction of the decade, grossing $200 million despite a $30 million budget. He is the new boy in town but do not discredit his skills and talent just because he is a new boy. He did a mesmerizing visual concept on Halo before the production goes blank over budgeting under the guidance of Peter Jackson. This could be a chance for him to step more into the mainstream world; since Halo doesn't work, maybe he deserves another chance. He employs a documentary-style, hand-held, cinéma vérité technique, blending naturalistic and photo-realistic computer-generated effects - a perfect ingredient for The Hobbit.
3) Andrew Adamson
He is not a well-known yet, but Adamson is the man behind the first two Chronicles of Narnia. The concepts of the story is perfectly made and taking advantage of the beautiful scenery of New Zealand. Although both are mixed-received, his ability to take things down smoothly into a live-action transition is well praised. Like Jackson, he is also from New Zealand. The fact that he made the Chronicles of Narnia should be enough reason for him to be in the contention. he also made Shrek and Shrek 2 - both of been the best on its series. He is one of the under-rated director in the Hollywood!
1) Christopher Nolan
This name is been thrown in the list because he is a great storyteller and a great filmmaker. Both are very important ingredients when you want to take The Hobbit into the silver screen and fortunate enough, he has. With his strong tied in with Warner Bros, this could be another one who should be considered seriously into taking the job. The problem is; he has show no interest in taking the job, besides he is fully occupied with his Batman and Superman franchise. Maybe if The Hobbit production is delayed by another five years he may be in. With his devotion to the DC Comics, can he still find some times for The Hobbit? Not really, just a nice possibility.
2) Steven Spielberg
His involvement will give the level of anticipation goes higher. Or else, his involvement will also speed up the process of production. No one can argue his importance in making the film in every genre tastes so good. He never had anything that goes down to the gutter - and known for his perfect touch on every film he directed. The problem is; Speilberg is not the type of person that will jump on board simply, unless he build it all up by himself. If he is in, expect the radical changes from top to bottom. Spielberg and Peter Jackson have been working together on the film adaptation of The Adventures of TinTin.
Best friends of Spielberg and they shared many common traits. JJ Abrams is a master in story and styles - add in some fantasy weirdness and practical action sequences (which are good), he is ready to make any fanboys wet on their pants. The problem is; The Hobbit could be the whole damn thing that deviates away from Abrams' comfort zone of action or pure science fiction. It would be interesting to see he tackles something out of ordinary and more fantasy. But what are the odds?
* I have not completed my part two of World Cup 2010 yet, stay tune....
* I have not completed my part two of World Cup 2010 yet, stay tune....


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November 13, 2016
listen to this show:
Add or read comments
Dark Spirits - The Red Temple Spirits (12" LP: Killed By Deathrock Vol 2) [Sacred Bones] 3:00
Road Runner (Listen: ) - Bo Diddley (45) 1960 [Checker] 3:0420
Promised Land (Listen: ) - The Skeletal Family (12" LP: Killed By Deathrock Vol 2) [Sacred Bones] 3:07
(Listen to your DJ: ) Music behind DJ:
(Listen: ) mic break: 3:1150
Cobra's Blood (Listen: ) - Kit Convict & Thee Terrible Two (CD: Cobra's Blood) [Off The Hip] 3:1340
I Need You There (Listen: ) - The Chessmen (45) 1965 [Bismarck] 3:1555
Kill The Lovers (Listen: ) - The Jangle Band (CD: Edge Of A Dream) [Off The Hip] 3:1815
Suzanne (Listen: ) - Leonard Cohen (12" LP: Songs Of Leonard Cohen) 1967 [Columbia] 3:2130
Kill A Clown (Listen: ) - Mike Daly & The Planets (CD: Out Of The Garage Vol. 2) [Bongo Boy] 3:2515
Wild As The Wind (Listen: ) - The Unit Four Plus Two (45) 1965 [London] 3:2805
(Listen to your DJ: ) Music behind DJ:
(Listen: ) mic break: 3:3015
24 Hours From London (Listen: ) - Little Murders (CD: Hi-Fab!) [Off The Hip] 3:3230
Kaw-Liga (Listen: ) - Hank Williams (45) 1953 [MGM] 3:3530
Don't Let Her Get Away (Listen: ) - Muck & The Mires (7" Split EP w/ The Fadeaways) [Dirty Water] 3:3805
She's No Good (Listen: ) - The Cyclones (45) 1965 [Lee] 3:4005
Corpse On A Roof (Listen: ) - The Blind Shake (12" LP: Celebrate Your Worth) [Goner] 3:4220
Number 9 Train (Listen: ) - Tarheel Slim (45) 1958 [Fury] 3:4545
(Listen to your DJ: ) Music behind DJ:
(Listen: ) mic break: 3:4845
Goodbye (Listen: ) - Jon & The Vons (12" LP: Gratest Hits Vol 1) [Soundflat] 3:5025
In Time (Listen: ) - The Sir Douglas Quintet (45) 1965 [Tribe] 3:5305
Pinkerton's Assorted Colours (Listen: ) - The Anderson Council (CD: Assorted Colours) [Jem] 3:5515
Gum Drop (Listen: ) - Otis Williams & The Charms (45) 1955 [Deluxe] 3:5845
Seconds Waiting (Listen: ) - The Strand (12" LP: Seconds Waiting) [Dig!] 4:0120
Making Time (Listen: ) - The Creation (45) 1966 [Planet] 4:0350
(Listen to your DJ: ) Music behind DJ:
(Listen: ) mic break: 4:0645
Out Of My Mind (Listen: ) - The Leonards (CD: Garage Sale) [Rock Ranch] 4:0910
Hippy Hippy Shake (Listen: ) - Chan Romero (45) 1959 [Del-Fi] 4:1205
The Other Side (Listen: ) - The Lovely Intangibles (CD: Air & Numbers) [The Lovely Intangibles] 4:1345
Games (Listen: ) - The Nova Local (45) 1967 [Decca] 4:1725
Burnin' Honey (Listen: ) - Patsy's Rats (45) [Dig!] 4:1930
Judy's Turn To Cry (Listen: ) - Lesley Gore (45) 1963 [Mercury] 4:2210
(Listen to your DJ: ) Music behind DJ:
(Listen: ) mic break: 4:2415
Jerkin' Back 'N' Forth (Listen: ) - The Kurt Baker Combo (CD: In Orbit) [Wicked Cool] 4:2650
Garden Of My Mind (Listen: ) - The Mickey Finn (45) 1967 [Direction] 4:2935
Take Back What I Need (Listen: ) - The Darts (Digital single) [The Darts] 4:3205
Big Ten Inch Record (Listen: ) - Bull Moose Jackson (45) 1952 [King] 4:3430
Mean Red Spider (Listen: ) - Greg "Stackhouse" Prevost (CD: Universal Vagrant) [Mean Disposition] 4:3645
Hurtin' For Your Love (Listen: ) - The Balloon Farm (45) 1968 [Laurie] 4:3910
(Listen to your DJ: ) Music behind DJ:
(Listen: ) mic break: 4:4110
You Got To Lose Me (Listen: ) - Jack Rivera (CD single) [Brooklyn Avenue] 4:4335
Love (Can Make You Happy) (Listen: ) - Mercy (45) 1969 [Sundi] 4:47
Singing Man (Listen: ) - The Beginner's Mynd (45) [Hidden Volume] 4:5015
I'm Crying (Listen: ) - The Animals (45) 1964 [MGM] 4:5255
Psychedelic Sea (Listen: ) - Jenny & The Felines (CD: Gnarly Wave) [Bongo Boy] 4:5535
Music behind DJ:
mic break: 4:5910
Listener comments!
Sun. 11/13/16 3:10pm
Bo-lieve It or Not:
from Marv Goldberg's extensive Five Keys post:
On November 20, 1955, Dr. Jive [Tommy Smalls] was booked on the newly-renamed "Ed Sullivan Show" (formerly "The Toast Of The Town") to bring 15 minutes of R&B into the nation's collective living room. He brought the 5 Keys (singing "Ling, Ting, Tong"; they had wanted to do "Close Your Eyes," but "Ling Ting Tong" was specifically requested by Ed Sullivan's daughters!), Lavern Baker, Willis "Gator Tail" Jackson's Orchestra, and Bo Diddley. For some unknown reason, Sullivan got it into his head that Bo Diddley should sing "Sixteen Tons," instead of one of his own songs. That afternoon, everyone showed up to rehearse, and Bo was kept practicing for around four hours (they even pasted the lyrics on the stage itself, so he wouldn't have to memorize them). However, when he got on stage, he simply decided to sing "Bo Diddley," with its pounding Juba beat, instead of "Sixteen Tons." This absolutely infuriated Sullivan (which Bernie [of the Five Keys] will attest to; they were standing near him in the wings) and he never had Diddley on again. (Of course, to put it into perspective, the 5 Keys did exactly what Sullivan told them to and he never had them on again either!)
Sun. 11/13/16 3:14pm
Mailman Tom:
R.I.P. Billy Miller
Sun. 11/13/16 3:23pm
Thank you for Suzanne!
Sun. 11/13/16 3:26pm
Revolution Rabbit Nov63:
What is there that can equal that? 'Visions of Johanna'?
Sun. 11/13/16 3:26pm
Mailman Tom:
R.I.P. Leon Russell
Sun. 11/13/16 3:29pm
Too many passings in the music world this year.
Sun. 11/13/16 4:09pm
looking stuff up:
"In 1958 Allen Bunn entered the recording studio again, this time renamed Tarheel Slim, under the aegis of producer Bobby Robinson and with Wild Jimmy Spruill on guitar and Horace Cooper on piano cut his greatest record, a two sided monster-- Wildcat Tamer b/w Number 9 Train, issued on Robinson's Fury label, it remains one of the pinnacles of New York rock'n'roll. Both sides feature Slim's burning guitar, with Spruill's scratchy rhythm guitar driving both tunes at full steam, they remain the type of classic performances that never sound old or dated . . . "
-- excerpt from James "The Hound" Marshall's blog
Sun. 11/13/16 4:17pm
Hi, what's the song played now?
Sun. 11/13/16 4:21pm
@Mailman Tom: Who is Billy Miller?
Sun. 11/13/16 4:22pm
looking stuff up:
what means now? the haunting female vocalist song was the lovely intangibles - "the other side" Or now might mean the one after that
Sun. 11/13/16 4:25pm
yes I realise now was not very helpful... sorry about that. But that was the one I meant, thanks! Really nice track :)
Sun. 11/13/16 4:36pm
looking stuff up remembers The Lost Patrol:
When Rochester native and former Dotsun Moon vocalist Mary Ognibene contacted The Lost Patrol's Stephen Masucci over the winter of 2014-2015 about experimenting with some music, the plan was to record a song or two. After coming to the conclusion that their first song sounded promising, Ognibene and Masucci (along with The Lost Patrol's Michael Williams and Tony Mann) decided to take the recording process one step further. Seven months later, the debut CD, Tomorrow is Never by The Lovely Intangibles is out for worldwide release.
"The intention was to record a cut or two, but shortly into the process, we realized the viability of putting out something a bit more substantive as a proper full-length. Michael and Tony were eager to press forward and brought in some ideas of their own, and we were also fortunate enough to enlist bass virtuoso Jon Camp (Renaissance) on a track." says Masucci.
The Lovely Intangibles expand upon musical themes set forth by The Lost Patrol, but take the music into breezier skies. Guitars still cascade with endless flutter into the reverb-drenched void while heart-stopping bass and synthesized soundscapes envelop you.
-- from lastFM
Sun. 11/13/16 4:40pm
Mailman Tom:
Billy Miller was in the Zantees and the A-Bones. A true rock and roll soul.
Sun. 11/13/16 4:59pm
looking stuff up:
In 2014 Animals organist Alan Price started a YouTube channel, where he posts home videos of some of his solo work. In addition, Price performs with his band, mostly at his long-established monthly spot at the Bull's Head, Barnes, London.
Thu. 11/17/16 1:53am
John from CDA:
Billy Miller was also co-founder of the Norton record label.
Thu. 11/17/16 9:04am
looking stuff up:
Ohhh! Well why didn't Mailman Tom say so? An occasional memorable Gaylord censored-comment-board show was entirely devoted to Norton Records, 3-24-2013
Three Chord Monte did a tribute this week
Thu. 11/17/16 9:11am
Hello, ball:
Ohhh! Well why didn't Mailman Tom say so? An occasional memorable Gaylord censored-comment-board show was entirely devoted to Norton Records 3-24-2013
Three Chord Monte paid tribute this Monday
Ohhh! Well why didn't Mailman Tom say so? An occasional memorable Gaylord censored-comment-board show was entirely devoted to Norton Records 3-24-2013
Three Chord Monte paid tribute this Monday
RSS feeds for Teenage Wasteland with Bill Kelly: Playlists feed | MP3 archives feed[ Teenage Wasteland ][ Playlists & Archives ][ Disgraceland ][ Disgraceland II ][ The Guru of the Garage ][ E-mail Bill Kelly ]


text:
To start, select
Sync Profiles from the menu in your Cloudwork dashboard
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Select the sync profile for your graduating year, and then change
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Submit. Go back into the sync profile and click
Start Full Resync. In a few minutes, your graduated students will all be marked as alumni. You can check this by examining any of the newly graduated students. You can also navigate to your dashboard home page and observe that the number of chargeable users has now been decreased (and of course the number of Non-chargeable users correspondingly increasing).
I only have one Sync Profile for my students!No problem! We need to pln how to setup new sync profiles for each of your graduating years. Call Studentnet on (02) 9281 1626 and we'll be happy to plan this process with you..
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We'll also accept a list of usernames to process if you manage your students directly or via CSV files instead of syncing with Active Directory.


text:
Mitochondria are essential eukaryotic organelles often forming intricate networks. The overall network morphology is determined by mitochondrial fusion and fission. Among the multiple mechanisms that appear to regulate mitochondrial fission, the ER and actin have recently been shown to play an important role by mediating mitochondrial constriction and promoting the action of a key fission factor, the dynamin‐like protein Drp1. Here, we report that the cytoskeletal component septin 2 is involved in Drp1‐dependent mitochondrial fission in mammalian cells. Septin 2 localizes to a subset of mitochondrial constrictions and directly binds Drp1, as shown by immunoprecipitation of the endogenous proteins and by pulldown assays with recombinant proteins. Depletion of septin 2 reduces Drp1 recruitment to mitochondria and results in hyperfused mitochondria and delayed FCCP‐induced fission. Strikingly, septin depletion also affects mitochondrial morphology in Caenorhabditis elegans, strongly suggesting that the role of septins in mitochondrial dynamics is evolutionarily conserved.
This study provides evidence that septins, conserved components of the cytoskeleton, are involved in Drp1‐mediated mitochondrial fission.
Septin depletion causes mitochondrial elongation in mammalian cells and in the nematode C. elegans.
Septin 2 directly interacts with the mitochondrial fission factor Drp1 and regulates its mitochondrial association.
Mitochondria are highly dynamic eukaryotic organelles, which form an interconnected and dynamic network. Mitochondrial morphology and function are tightly interrelated. The dynamic behavior of mitochondria relies partly on their movement along cytoskeletal tracks, including microtubules, intermediate filaments, and actin (reviewed in [1]). In addition, mitochondrial network dynamics is determined by mitochondrial fusion and fission, to which the cytoskeleton also contributes. The molecular basis of mitochondrial dynamics is intensely studied from both the medical and the fundamental cell biology standpoints, as defects in this process can lead to mitochondrial dysfunction and to pathology [2], [3], [4], [5]. The canonical mitochondrial fission mechanism involves the dynamin‐like protein Drp1, which is recruited from the cytosol to mitochondria, where it oligomerizes to form spirals that constrict the mitochondrion [6]. Drp1 recruitment and oligomerization are highly regulated at the molecular level through posttranslational modifications of Drp1 (reviewed in [7]) and through the presence of several Drp1 receptors on the mitochondrial outer membrane [8], [9], [10], [11], [12], [13], [14]. Drp1‐mediated fission is facilitated by ER tubules that wrap around mitochondria and constrict them. An ER‐localized formin, inverted formin 2 (INF2) [15], and a mitochondria‐localized Spire1 isoform (Spire1C) [16] cooperate to induce localized actin polymerization at the constriction sites [16]. In addition to this, several actin‐binding proteins have been reported to participate in mitochondrial fission [17], [18]. The motor protein myosin 2 (Myo2), proposed to provide actin with the necessary contractile force for mitochondrial fission ("mitokinesis" [18]), is known to not only interact with actin, but also with septins [19]. Septins are conserved eukaryotic GTP‐binding proteins that are considered components of the cytoskeleton as they can form non‐polar filaments (reviewed in [20], [21]). Septins are highly expressed in interphase cells [22], suggesting that they play a role beyond their well‐established contribution to cytokinesis [23], [24]. Indeed, recent studies indicate that septins are involved in a number of different cellular processes, such as ciliogenesis [25], [26], axon guidance [27], and phagocytosis, [28] and serve to restrict bacterial actin‐based motility [29] and protein diffusion at the cell cortex [30], at the yeast bud neck [31], and at the ER [32]. Knockout of any of the 13 mammalian septins leads to highly diverse phenotypes, further indicating that different septins may perform different functions [21]. Mammalian septins are subdivided into 4 groups (septin 2, septin 3, septin 6, septin 7) and form heteropolymeric complexes with the ubiquitous septin 2 (Sept2) at the heart of the complexes [20]. Septins display a complex localization pattern: Long fibers occur under the nucleus and along actin stress fibers, while both long and short fibers are found throughout the cell, accumulating at the cell periphery in association with actin (reviewed in [20]). Furthermore, ringlike structures decorate the cytosol and subcortical regions [33].
In contrast to actin filaments and microtubules, septins are not known to interact extensively with mitochondria. To our knowledge, a single report indicated that septins colocalize with mitochondria in the ciliate Tetrahymena thermophila and septin depletion/overexpression grossly disrupted mitochondrial morphology in this organism [34]. Nevertheless, the mechanism through which ciliate septins act in mitochondrial dynamics has remained elusive. Concerning mammalian septins, knockout of the differentially expressed septin 4 (Sept4) in mice has been shown to result in sperm defects, including aberrant annulus and mitochondrial architecture [35]. Two Sept4 splice isoforms have furthermore been found to localize to mitochondria and participate in apoptosis and neuronal development, respectively [36], [37]. To date, it is unclear whether any of the ubiquitously expressed septins is involved in mitochondrial dynamics of metazoan cells.
Here, we show that in mammalian cells Sept2 directly interacts with the mitochondrial fission protein Drp1 and is required for efficient localization of Drp1 at mitochondria, thus introducing septins as new players in mitochondrial dynamics.
Sept2 depletion induces mitochondrial elongation
We assessed the role of septins in mitochondrial dynamics by silencing three members of the family (i.e., Sept2, Sept7, and Sept9) and analyzing mitochondrial morphology through indirect immunofluorescence (Fig 1A). Mitochondria were significantly elongated in Sept2‐ and Sept7‐silenced cells compared to control cells, respectively, by 1.8‐fold and 1.4‐fold. In contrast, mitochondrial length did not significantly increase in Sept9‐depleted cells (Fig 1B). Previous studies have shown that depletion of Sept7 codepletes Sept2 [33], [38], [39], which could explain why depletion of either Sept2 or Sept7 causes an increase in mitochondrial length. We therefore assessed the levels of Sept2 upon Sept2, Sept7, and Sept9 depletion. In our hands, the depletion efficiency of Sept2 reached almost 90%, while that of Sept7 reached 80% and resulted in a concomitant 65% decrease in Sept2 levels, in agreement with previous reports [33], [38], [39]. In contrast our very efficient Sept9 depletion (97%) did not significantly co‐down‐regulate Sept2 (Fig EV1A–D). These findings are consistent with our observation that the depletion of Sept2 and Sept7, but not that of Sept9, affects mitochondrial length.
Source Data for Figure 1 [embr201541612-sup-0003-SDataFig1.zip]
Given the strong mitochondrial phenotype obtained upon Sept2 depletion, we decided to focus our attention on Sept2 and its possible involvement in mitochondrial dynamics. To ensure that the observed mitochondrial elongation in Sept2‐depleted cells is not due to an off‐target effect, we confirmed the phenotype with different Sept2‐targeting siRNA sequences (Fig EV1E and F) and in different cell types (HeLa, Fig 1A; and U2OS, Fig 2D). Furthermore, the mitochondrial elongation phenotype of Sept2‐silenced cells could be rescued through overexpression of siRNA‐resistant Sept2 (Fig 1C and D). Interestingly, Sept7 overexpression could also rescue the mitochondrial elongation phenotype induced by Sept2 siRNA, albeit less efficiently compared to the Sept2 siRNA‐resistant construct, that is, 54% rescue upon Sept7 overexpression compared to 70% rescue for the overexpression of siRNA‐resistant Sept2 (Fig 1D). These results further suggest that both proteins play a role in mitochondrial dynamics (see Discussion).
Source Data for Figure 2 [embr201541612-sup-0004-SDataFig2.zip]
We next asked whether increasing the amount of Sept2 would induce mitochondrial fission. Similar to Drp1 overexpression [40], overexpression of HA‐tagged Sept2 did not substantially induce mitochondrial fragmentation (our unpublished results), consistent with the notion that mitochondrial fission is a well‐controlled multifactorial process, with multiple rate‐limiting factors.
Since septins have been implicated in ER polarization in yeast [32], we sought to determine whether Sept2 depletion would affect the morphology of the ER or of other organelles, such as the Golgi apparatus and peroxisomes. The ER and Golgi morphology was not visibly altered in Sept2‐depleted cells (Fig 1E). Similar mean areas were detected for the Golgi and the ER–mitochondria overlap in mock‐ and siRNA‐treated cells (Fig 1F and G). Moreover, live cell imaging showed that the ER marked mitochondrial fission sites to the same extent in control and in Sept2 siRNA‐treated cells (Fig EV1G). Likewise, the number and size of peroxisomes were not significantly changed in Sept2‐depleted cells compared to mock‐treated cells (Fig 1E and H), suggesting that although mitochondria and peroxisomes share components of the fission machinery [13], [41], Sept2 acts specifically in mitochondrial dynamics.
Sept2 does not affect fusion but controls mitochondrial fission
The mitochondrial hyperfusion phenotype could result from an increase in fusion activity or a decrease in fission. We thus tested whether Sept2 depletion would affect global levels of the key mitochondrial dynamics proteins Mfn1/2 and Drp1. Total levels of Mfn1/2 and Drp1 were not affected by Sept2 siRNA (Fig EV2A and B). Live cell imaging revealed that Sept2‐depleted cells displayed an increase in mitochondrial motility (Fig EV2C and D), which may allow more frequent encounters between mitochondria and thus higher fusion rates [15]. We therefore tested whether Sept2 stimulates mitochondrial fusion activity. To measure the mitochondrial fusion rate, we employed an established assay, in which mitochondrial photoactivatable GFP (mito‐PA‐GFP) is activated in a small region of interest, and its decay in fluorescence is followed over time to estimate the number of mitochondrial fusion events (i.e., the fusion rate), from which the fluorescence decay depends [42]. Using this assay, we could not detect any significant increase in mitochondrial fusion when comparing Sept2‐silenced cells with mock‐treated cells (Fig 2A and B).
Given that Sept2 depletion resulted in elongated mitochondria without increasing mitochondrial fusion, we analyzed whether Sept2 functionally contributes to mitochondrial fission by measuring fission rates in mock‐treated and Sept2‐depleted cells. Sept2‐depleted cells display decreased fission rates (mean 14.8 ± 5.7) compared to mock‐treated cells (21.97 ± 10.8; Fig 2C). Sept2‐depleted cells also differed with respect to FCCP‐induced mitochondrial fission and looping [43], which appeared delayed in Sept2‐depleted cells compared to cells treated with control siRNA (Fig 2D and E). Given that FCCP‐induced mitochondrial fission is Drp1‐dependent [44], [45], [46], [47], [48], [49], this suggests that Sept2 may play a role in Drp1‐dependent mitochondrial fission.
Sept2 localizes at mitochondrial fission sites
We reasoned that given the effect of Sept2 depletion on mitochondrial morphology and its implication in FCCP‐induced mitochondrial fission, a fraction of Sept2 would localize at mitochondria to regulate mitochondrial dynamics. We thus inspected the localization of endogenous Sept2 by confocal microscopy and found Sept2 structures at mitochondria in HeLa (Fig 3A and B) and U2OS cells (Fig 3C). In addition, we detected Sept2 in a crude mitochondrial fraction (Fig EV3A). 3D image reconstruction showed that endogenous Sept2 localizes above and around constricted mitochondria (Fig 3B, white arrowheads). Notably, we also found constrictions that were not marked by Sept2 (Fig 3B, yellow arrowhead). This might be due to: (i) a transient association of Sept2 to mitochondrial fission sites; (ii) participation of Sept2 to only a subset of fission events, similar to what has been suggested for Myo2 and INF2 [15], [18]; or (iii) that other mechanisms ensure mitochondrial constriction in parallel. To achieve a clearer view of Sept2 structures at mitochondria we turned to super‐resolution imaging, using two complementary super‐resolution approaches: Bioaxial's conical diffraction (CoDiM) based super‐resolution method (Fig 3C) [50] and structured illumination microscopy (SIM) (Fig EV3B). Where mitochondria were not obscured by strong cytosolic Sept2 staining, we detected Sept2 on 36% of constriction sites (n = 209 constriction sites), with short Sept2 structures often traversing the mitochondria in a perpendicular way (Fig 3C).
Source Data for Figure 3 [embr201541612-sup-0005-SDataFig3.zip]
We then monitored the dynamics of Sept2‐YFP localization on mitochondria and in agreement with immunofluorescence analysis found Sept2 associated with mitochondria at prospective fission sites. We detected both discrete Sept2 puncta as well as Sept2‐enriched halos on prospective mitochondrial fission sites (Fig 3D). Collectively, these results indicate that Sept2 localizes to mitochondria and is involved in mitochondrial fission.
Sept2 interacts directly with Drp1
We then sought to determine how Sept2 would act on mitochondrial fission. Given that Drp1‐dependent mitochondrial fission is delayed in Sept2‐depleted cells (Fig 2D and E), we hypothesized that Sept2 might interact with Drp1. Consistent with this hypothesis, immunoprecipitation of endogenous Sept2 resulted in co‐precipitation of endogenous Drp1 from cell lysates of several different human cell types (HeLa, HEK293, and U2OS), indicating in vivo association of Drp1 and Sept2 (Figs 4A and EV3C and D). In contrast, actin or the mitochondrial outer membrane protein Tom20 did not co‐precipitate in HeLa cells (Fig 4A), nor did INF2 in U2OS cells (Fig EV3D). To further verify the specificity of the Sept2–Drp1 interaction, we immunoprecipitated an unrelated cytosolic protein (PI4KIIα) and did not recover any Drp1 (Fig EV3E). The interaction between Sept2 and Drp1 is likely to be direct because recombinant Sept2 interacted with Drp1 in GST pulldown experiments (Fig 4B). Immunofluorescence and live cell imaging confirmed that Sept2 and Drp1 can be found together at mitochondria (Fig 4C) and at mitochondrial fission sites (Fig 4D). We noticed that the two proteins do not colocalize at all prospective fission sites, suggesting that either the Sept2–Drp1 complex forms very transiently, or that Sept2 is below the detection level at these sites, or that Drp1 is recruited to mitochondria through several independent pathways. To investigate whether the Drp1–Sept2 interaction occurred preferentially in the cytoplasm or on mitochondria, we treated cells with CCCP to increase Drp1 localization to mitochondria. Treatment with CCCP caused a twofold increase in Drp1 binding to Sept2, suggesting that formation of the Sept2‐Drp1 complex is stimulated at mitochondria (Fig 4E and F). We next asked whether the interaction between Sept2 and Drp1 was sensitive to the activation state of Drp1. We thus treated cells with Mdivi‐1, which inhibits self‐assembly and GTPase activity of Drp1, resulting in increased interconnectivity of the mitochondrial network [51] (Fig EV4F) without depolymerizing Sept2 filaments (our unpublished results), and then performed Sept2 immunoprecipitation. Mdivi‐1 treatment decreased the association between Sept2 and Drp1 by almost 50% in intact cells (Fig 4G and H), and partially decreased the Sept2–Drp1 interaction in an in vitro pulldown assay (Fig EV3G).
Source Data for Figure 4 [embr201541612-sup-0006-SDataFig4.zip]
Together, these data establish that Sept2 directly interacts with Drp1 in vivo and in vitro and suggest that the activation state of Drp1, its assembly, or the molecular environment on the mitochondrial outer membrane may stabilize the Sept2–Drp1 complex.
Mitochondrial localization of Drp1 depends on Sept2
Since Sept2 interacts with Drp1, we analyzed the effect of Sept2 depletion on the mitochondrial localization of Drp1 complexes. Immunofluorescence analysis showed that the amount of mitochondria‐associated Drp1 significantly decreased in Sept2‐silenced cells (67% ± 15.1) compared to mock‐treated cells (Fig 5A and B). In concomitance, the average distance between Drp1 clusters increased by a factor of 1.6 in Sept2‐silenced cells (Fig 5C), indicating a decrease in Drp1 cluster density along mitochondria. In addition, Western blotting analysis of mitochondria isolated from cells treated with control siRNA or with Sept2 siRNA confirmed a decrease in the amount of mitochondria‐associated Drp1 (Fig 5D). These data suggest that Sept2 acts upstream of Drp1, similar to what has been recently shown for INF2 and Myo2 [15], [18]. In agreement with this finding, Drp1 depletion did not affect Sept2 levels or subcellular distribution, as assessed by immunofluorescence (our unpublished results). Altogether, our observations revealed that Sept2 participates to the localization of Drp1 complexes on mitochondria.
Source Data for Figure 5 [embr201541612-sup-0007-SDataFig5.zip]
Mitochondria‐associated actomyosin is not perturbed upon Sept2 depletion
Actin and myosin 2 (Myo2) have been recently proposed to act together in mitochondrial preconstriction, favoring Drp1 accumulation in order to accomplish fission [18]. Myo2 had also been shown previously to directly bind Sept2 [19]. This binding was required for phosphorylation‐dependent activation of Myo2, which played an important role in stress fiber maintenance and during cytokinesis [19]. However, whether the Sept2–Myo2 interaction can also occur at mitochondria and/or have a functional role in mitochondrial dynamics is unknown to date. Therefore, we explored whether Sept2 depletion would alter Myo2 localization at mitochondria or decrease its activation. We neither detected changes in total Myo2A or Myo2B levels (Fig EV4A and B) nor a decrease in the levels of phosphorylated regulatory myosin light chain (P‐MLC and PP‐MLC; Fig EV4C–F), which determines the actin‐binding properties of Myo2 in Sept2‐silenced cells [52]. We also failed to detect a significant change in mitochondria‐associated P‐MLC by immunolocalization (Fig 6A and B). We thus concluded that the mitochondrial elongation observed in Sept2‐depleted cells is not due to perturbation of Myo2 levels or activity.
Source Data for Figure 6 [embr201541612-sup-0008-SDataFig6.zip]
Notably, P‐MLC has been shown to localize to mitochondria in an actin‐dependent manner [18]. The lack of P‐MLC redistribution in Sept2‐depleted cells suggests that the interaction of actin with mitochondria is not perturbed in this context. In agreement with this observation, we found that Sept2 depletion did not interfere with the induction of actin recruitment to mitochondria upon FCCP treatment [17] (Fig 6C). In addition, we examined whether Sept2 depletion would affect the mitochondrial localization of the actin‐binding proteins Arp3, cofilin, and cortactin, which were recently shown to regulate mitochondrial morphology [17]. Immunofluorescence and cell fractionation experiments showed that the mitochondrial association between Arp3, cofilin, and cortactin was not affected in Sept2‐depleted cells (Fig 6D and E).
We next tested whether the interaction between Sept2 and Drp1 requires actin polymerization by treating cells with cytochalasin D prior to immunoprecipitation and found that the Sept2–Drp1 interaction was not affected (Fig 6F). Together, these results indicate that Sept2 is not involved in the recruitment of actin and Myo2 at mitochondria and that the interaction between Sept2 and Drp1 is independent of actin dynamics.
Septin contribution to mitochondrial dynamics is conserved in C. elegans
To understand whether the septin contribution to mitochondrial dynamics is conserved among metazoa, we turned to C. elegans, which possesses only two septins, UNC‐59 and UNC‐61. We inspected mitochondrial morphology in body wall muscle cells, a well‐established model to assess mitochondrial dynamics phenotypes [53], [54], [55]. Knockdown of either UNC‐59 or UNC‐61 or both by siRNA dramatically affected mitochondrial morphology, causing an increase in mitochondrial branching and length (Figs 7A and B, and EV5A and B) that is reminiscent of fission protein knockdown [54], [55], [56]. We then assessed whether combining the depletion of septins with the depletion of Drp1 would result in a further increase in mitochondrial length, which would indicate that the two proteins act on mitochondria through different pathways. We chose to focus on UNC‐61, whose depletion results in a stronger mitochondrial phenotype compared to UNC‐59, and co‐silenced it with DRP‐1. The strong mitochondrial phenotype of DRP‐1(RNAi) worms was not exacerbated when UNC‐61 was co‐silenced, suggesting that the phenotypes induced by DRP‐1 and UNC‐61 knockdown are not additive (Fig 7C). These data are consistent with the phenotype observed in mammalian cells and suggest that the function of septins in mitochondrial dynamics is conserved in C. elegans.
Source Data for Figure 7 [embr201541612-sup-0009-SDataFig7.zip]
Mitochondrial fission is a highly regulated process, and recent progress has highlighted novel roles for several cellular components in this process, such as the ER, actin, and multiple actin‐binding proteins [15], [16], [17], [18], [43], [57]. Here, we add a new player to this complex picture and report on the role of septins, a component of the cytoskeleton, in mitochondrial dynamics. We describe that septin expression is important for mitochondrial morphology in human cell lines and in the nematode C. elegans. In human cells, depletion of three ubiquitously expressed septins (Sept2, Sept7, and Sept9) showed that both Sept2 and Sept7 are important for mitochondrial fission, as their depletion induces significant mitochondrial elongation. In line with previous results [33], [38], [39], we found that that depletion of Sept7 partially codepletes Sept2. The remaining Sept2 may account for the decreased penetrance of the mitochondrial phenotype in Sept7‐silenced cells compared to Sept2‐silenced cells. Furthermore, the small levels of Sept2 remaining after Sept2 siRNA may serve as seeds that allow Sept7 to partially rescue the mitochondrial phenotype when overexpressed in Sept2‐silenced cells. In contrast, Sept9 depletion did not induce mitochondrial elongation, but appeared to increase mitochondrial interconnectivity, similar to nocodazole treatment [43], [58]. We speculate that the differences between Sept2/7 and Sept9 may be related to the position of Sept9 in the septin heterocomplex [59] or to the presence of Sept2‐containing complexes in Sept9‐depleted cells that still act on mitochondria. Such Sept2‐containing complexes were previously shown to form short filaments in Sept9‐depleted cells [59]. In agreement with this, we did not detect Sept2 codepletion in Sept9‐silenced cells.
In our hands, Sept2 presented the most dramatic mitochondrial phenotype, raising the question of how Sept2 mechanistically impacts mitochondrial dynamics. We propose that Sept2 plays a role in Drp1‐dependent mitochondrial fission. This notion is supported by several observations: First, mitochondrial association of Drp1 is impaired in Sept2‐depleted cells. Second, FCCP‐induced mitochondrial fission, which is Drp1‐dependent, is delayed in Sept2‐depleted cells. Lastly, Sept2 interacts directly with Drp1. An indirect confirmation of our results is the recent finding that Sept5 and Sept11 bind dynamin in neuronal cells [60]. The high homology of dynamin and Drp1 suggests that their interaction with septins may be ancestral. The interaction between Sept2 and Drp1 appears to require Drp1 assembly or activation, as it is sensitive to Mdivi‐1, which has been shown to inhibit the Drp1 assembly‐dependent GTPase activity [51]. In our hands, the effect of Mdivi‐1 was stronger in vivo than in vitro, indicating that the molecular environment on the mitochondrial outer membrane may stabilize the Sept2–Drp1 complex.
In contrast, Sept2‐mediated GTP hydrolysis may be dispensable for this interaction, as overexpression of a GTPase‐deficient point mutant (Sept2‐T78G) did not affect mitochondrial morphology (our unpublished results), consistent with the very low rate of GTP hydrolysis that has been observed by others in septin heterooligomers [61], [62] and which appears to be due to the low solvent accessibility of the nucleotide‐binding pocket [63].
We propose alternative but not mutually exclusive scenarios that could account for the role of Sept2 in Drp1‐dependent mitochondrial fission. Sept2 could promote Drp1 recruitment by contributing to mitochondrial constriction. Alternatively, Sept2 might stabilize productive fission complexes or act as a scaffold that promotes Drp1 recruitment or retention on mitochondria, as has been proposed for actin [43], [57]. Notably, despite the fact that septins can use actin as an assembly template and partially colocalize with actin [33], we suspect that Sept2 does not play a substantial role in actomyosin driven mitochondrial fission [18]. In agreement with this hypothesis, actin and INF2 were not detected in Sept2 immunoprecipitations and cytochalasin D treatment had no effect on the Sept2–Drp1 interaction, the latter suggesting that it does not require actin polymerization. Furthermore, neither the total levels of Myo2A, Myo2B, P‐MLC or PP‐MLC, nor the localization of P‐MLC on mitochondria, which relies on actin, are affected in Sept2‐depleted cells. P‐MLC and PP‐MLC were reported to decrease in previous Sept2‐depletion experiments [19], a variation that may be explained by differences in the employed cell types (CHO‐K1 versus HeLa and U2OS) or silencing techniques (shRNA versus siRNA). Interestingly, Li et al [17] reported increased Drp1 recruitment to mitochondria upon depletion of the actin regulating proteins Arp3, cortactin, and cofilin, while we observe a decreased localization of Drp1 to mitochondria in Sept2‐depleted cells and no substantial difference in mitochondrial localization of Arp3, cortactin, and cofilin. Consistent with our findings, FCCP‐induced recruitment of actin to mitochondria was also preserved in Sept2‐depleted cells.
Our data support the view that multiple mechanisms regulate mitochondrial fission. On the one hand, accumulating evidence suggests the presence of Drp1‐independent fission mechanisms [48], [64]. On the other hand Drp1 is regulated in a highly complex manner, that is, through several Drp1 receptors on mitochondria, through posttranslational regulation of Drp1 itself, and through the regulated recruitment of Drp1 to mitochondria by its receptors Fis1, Mid49/51, and Mff [11], [12], [10], [13], [14], [65], [66]. The recruitment and activity of Drp1 on mitochondria is furthermore regulated by cellular structures such the ER [67] and actin [15], [18], [43], [57], as well as septins, which appear to function in parallel to the ER and actin. Hence, the mitochondrial fission process appears to be regulated in complex and at least partially redundant ways. Such redundancy is frequently observed in biological systems and is thought to contribute to their robustness. Although not the only means to recruit Drp1 to mitochondria, Sept2 has in contrast to the ER and actin the unique ability to directly bind Drp1. This could allow Sept2 to act as a scaffold that would promote functional interactions with other proteins on mitochondria, similar to the function that septins have already been shown to fulfill in other contexts such as cytokinesis [19]. Our findings thus unveil a new facet of Drp1 regulation and expand the role of the cytoskeleton in mitochondrial fission to septin proteins.
Materials and Methods
Cell culture and transfection
HeLa and U2OS cells were obtained from ATCC and cultured under standard conditions; media and additives were from Invitrogen. U2OS‐GFPSec61 stable cells were described previously [68], as well as Drp1−/− cells [45]. All cells were tested for mycoplasma (negative). siRNAs were transfected for 72 h at a final concentration of 12 nM with Lipofectamine RNAiMAX according to the manufacturer's instructions. Septin siRNA sequences and providers are detailed in Appendix Table S1; siRNA#1 and siRNA#2 were employed for Figs 1 and EV1A and B, while siRNA#1 is shown in subsequent experiments. For rescue experiments, HeLa cells were transfected with Sept2 siRNA (siRNA #1) for 48 h and then transfected for a further 24 h (using FuGENE) with a Sept2‐HA plasmid carrying silent mutations in the siRNA seed region (Sept2‐HA rescue; see Appendix Table S2) or its GTPase‐deficient derivative (carrying the T78G mutation [63]) or Sept7‐HA.
Plasmids, chemicals, and recombinant proteins.
Sept2‐HA template for cloning of siRNA‐resistant Sept2‐HA was provided by David Ribet. See Appendix Table S2 for construct and primer information. PA‐GFP and Sept2‐YFP were described previously [42], [69]. Orange and Deep Red Mitotracker, 100 nm TetraSpeck beads AlexaFluor‐labeled phalloidin and secondary antibodies were purchased from Invitrogen. Mdivi‐1 was obtained from Enzo Life Sciences, and all other chemicals were obtained from Sigma. Complete mini EDTA‐free protease inhibitor and PhosSTOP phosphatase inhibitor tablets were from Roche. Reduced glutathione was obtained from Sigma, and glutathione sepharose beads and protein A were from GE Healthcare. Recombinant purified GST and Drp1‐GST were prepared as described previously [70], and Sept2 was obtained from Cusabio (CSB‐EP617994HU).
Antibody sources are detailed in Appendix Table S3. All antibodies were used according to the manufacturer's instructions unless otherwise stated.
Cell fractionation, immunoprecipitations, and pulldown
Crude mitochondrial extracts were obtained through cell fractionation according to [71]. Immunoprecipitation was performed as described [29] with modifications. Briefly, HeLa cells were washed twice in phosphate‐buffered saline (PBS) and lysed for 30 min with 1 ml lysis buffer/10‐cm dish (20 mM Tris, pH 8.0, 150 mM NaCl, 10% glycerol) supplemented with 1% Igepal, protease, and phosphatase inhibitors. Lysis and all subsequent steps were performed at 4°C. The lysate was clarified (13,000 g, 10 min), and the protein concentration of the supernatant was determined by Bradford assay (Pierce). One milligram of lysate was incubated overnight with 2 μg of anti Sept2 antibody. A total of 30 μl of protein A–sepharose beads was added for 1 h, and the immune complexes were retrieved by centrifugation (500 g, 5 min). After four washes with lysis buffer, bound protein was eluted from the beads by boiling for 10 min in 30 μl Laemmli buffer. The eluate was analyzed by gradient SDS–PAGE (Bio‐Rad) and subjected to Western blotting via wet transfer to 0.45‐μm nitrocellulose membrane (Millipore). A total of 10 μg total lysate was loaded (corresponding to 1/100) for the input, 70–90% immunoprecipitated material to reveal interactors such as Drp1, or 5% immunoprecipitated material to reveal immunoprecipitated Sept2. Sept2 immunoprecipitations from drug‐treated cells were performed as above except that cells were either mock‐treated (DMSO or ethanol, respectively) or treated with either 50 μM Mdivi‐1 for 1 h (Mdivi‐1 was also included in the lysis buffer) or 2 μM CCCP for 30 min in MEM without serum, or 2 μM cytochalasin D for 30 min in MEM before cell lysis.
GST pulldown.
All steps were performed at 4°C. Equimolar concentrations of GST, GST‐Drp1 and Sept2 (500 nM) were incubated overnight in GST incubation buffer (20 mM Tris, pH 8.0, 1 mM EDTA, 0.2% Igepal, 100 mM KCl). A total of 30 μl of protein A–sepharose beads was added for 1 h; beads were then recovered by centrifugation (500 g, 5 min) and washed four times in GST incubation buffer. Bound protein was eluted from glutathione Sepharose beads with GST elution buffer (100 mM Tris, pH 8.0, 20 mM glutathione, 5 mM dithiothreitol). Eluates were analyzed as described above. To test the effect of Mdivi‐1 on the Drp1–Sept2 interaction, Drp1‐GST was preincubated with Mdivi‐1 for 30 min at RT before the addition of recombinant Sept2, followed by the pulldown procedure described above.
Imaging and image analysis
Immunofluorescence and live cell imaging were performed essentially as described in [48], [72]. For super‐resolution microscopy, cells were seeded onto high‐precision coverslips (Marienfeld), and after immunofluorescence, samples were mounted in SlowFade Gold (Invitrogen) or Fluoromount G. Super‐resolution structured illumination (SR‐SIM), providing an expected resolution of about 140 nm, was performed on a Zeiss LSM780 Elyra PS1 (Carl Zeiss, Germany) using 63×/1.4 oil Plan Apo objective. Three angles of the excitation grid with five phases each were acquired for each channel and each z‐plane. SIM images were processed with ZEN software for brightness/contrast adjustment. Images were aligned using 100 nm TetraSpeck beads embedded in the sample. The confocal image in Fig EV3B was acquired using the same equipment as the SR‐SIM and the same pixel size to allow comparison. Huygens professional software was used for deconvolution and brightness/contrast in Fig EV3B. CoDIM imaging was performed on a Bioaxial CoDiM 100 system equipped with 488, 561, and 640 lasers and decreases the resolution limit to about 80–100 nm. Imaris 6.4.1 (Bitplane) was used for 3D reconstructions.
Image analysis was performed with ImageJ unless otherwise stated. For morphometric analysis on fixed cells, mitochondrial length was assessed in well‐resolved mitochondria only; fissions were counted manually in live cell imaging experiments and displayed according to [57]. To calculate the percentage of rescue, the difference in mitochondrial length between Sept2‐siRNA and Sept2‐rescue or Sept7‐rescue is referred to the difference between Sept2‐depleted and mock‐treated cells (set to 100%). To quantify FCCP‐induced mitochondrial fission and looping, mitochondrial elongation (long/short axis) was measured with the open‐source software Icy [73] (protocol Mitochondria Elongation) after binarization of mitochondria. The first timepoint (t0) was set to 100%, and the following timepoints were normalized to t0. Mitochondrial constrictions were determined through visual inspection of the mitochondrial channel, and then, septin presence was assessed at these sites. Total mitochondrial motility was determined in ImageJ according to [74]. Peroxisomes were counted automatically with ImageJ particle analyzer. The percentage of ER crossing mitochondria was assessed after thresholding and binarization of the ER and mitochondrial signal according to [67]. Similarly, mitochondrial Drp1 was obtained by combining total Drp1 signal with a mitochondrial mask created after thresholding and binarization of the mitochondrial signal. Colocalization of P‐MLC was assessed similarly and verified using the plug‐in Colocalization Studio (Plug‐in ID: ICY‐H9X6X2) [75] in Icy. Western blot quantification involved densitometric analysis of single bands through ImageJ. At least two Western blots from independent experiments were quantified.
Statistical analysis
Results are expressed as means of at least two independent experiments, and error bars represent the standard error of the mean. For multiple comparisons, data were first analyzed by one‐way ANOVA on BiostaTGV (http://marne.u707.jussieu.fr/biostatgv/?module=tests), followed by pairwise comparisons with unpaired two‐tailed Student's t‐test on Excel (Microsoft) or Prism (GraphPad). Significance is indicated as P < 0.05 (*), P < 0.01 (**), and P < 0.005 (***), ns for P > 0.05.
C. elegans work
Caenorhabditis elegans was cultured and maintained as described previously [76] at 20°C. The worm line carrying pMyo3::Tom70::GFP, rol‐6 as extrachromosomal array was created previously [54]. RNAi was performed as described [77]. Plasmid L4440 containing unc‐59 (W09C5.2) or unc‐61 (Y50E8A.4) was retrieved from the Ahringer library [77] or the Vidal library [78], respectively. Both clones were sequenced to confirm their identity. For the double RNAi and the dilution controls, equal volumes of bacterial cultures were mixed, concentrated by centrifugation, and spread on NGM plates containing 1.5 mM IPTG and 25 μg/ml carbenicillin. dsRNA production was induced at RT for 16–24 h and then stored at 4°C for up to 2 weeks. TOM70::GFP worms were subjected to RNAi from L3/L4 stage for 4–5 days at 20°C. Offspring were analyzed at young adult stage. For live imaging, adult hermaphrodites were mounted in M9 containing 1 mM levamisole (Sigma‐Aldrich), using Vaseline® at the edges of the coverslip to function as a spacer. Worms and mounting medium were strictly kept at 20°C until use. Images were taken at a Zeiss Axioplan 2 microscope equipped with a Zeiss AxioCam MRm camera (Carl Zeiss, Germany) and a Plan Neofluar 100×/NA1.30 oil objective.
Author contributions
AP and FS conceived, performed, and interpreted the experiments; TNT and JKS performed and interpreted the experiments; RP, SO, ASa, and TL provided expertise; J‐CO‐M supervised TL; FS wrote the manuscript; AP, ASp, and PC reviewed and edited the manuscript; PC provided strong support and supervised the project.
Conflict of interest
The authors declare that they have no conflict of interest.
Expanded View
Expanded View Figures PDF [embr201541612-sup-0002-EVFigs.pdf]
Source Data for Expanded View [embr201541612-sup-0010-SDataEV.zip]
We would like to thank David Ribet for providing the Sept2‐HA template and Sept7‐HA and for helpful discussions, Henry Higgs for the kind gift of anti‐INF2 antibody, Barbara Pomili and the C3BI open desk for help with statistic analysis, Edith Gouin and Véronique Villiers for antibody purification, José Santos for help with mitochondrial fractionations, Béatrice de Cougny (Communication and Image Unit, Institut Pasteur) for help with the schematic illustration, and Serge Mostowy for providing initial siRNA‐treated samples and discussion. We gratefully acknowledge the kind financial support of the Institut Pasteur (Paris), the France‐BioImaging infrastructure network supported by the French National Research Agency (ANR‐10–INSB–04, Investments for the future), and the Région Ile‐de‐France (program DIM‐Malinf). TL is funded by a Bourse Roux fellowship from Institut Pasteur and the Labex IBEID. FS is a CNRS senior research fellow, and PC is a Senior International HHMI Scholar. Work in the Spang Laboratory is funded by the University of Basel and Swiss National Science Foundation (CRSII3_141956). The Cossart Laboratory is supported by Institut Pasteur, Inserm (U604), ANR (Grant 12‐BSV3‐0017‐03 Mitopatho), and ERC (Grant 670823 BacCellEpi).
FundingInstitut Pasteur (Paris)
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs 4.0 License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
- © 2016 The Authors. Published under the terms of the CC BY NC ND 4.0 license


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Major banks called in to broker Activision sale
Barclays and Goldman Sachs have been called into to help Vivendi secure the $8.1bn sale of Activision Blizzard, reports the Wall Street Journal.
Vivendi's share price shot up on reports it is looking to sell the Call of Duty and World of Warcraft publisher.
Rumours that Vivendi was planning to sell its 61 per cent stake in the profitable games publishing arm has been circulating for weeks now. The media conglomerate has a high level of debt and a flagging share price, the sale of Activision Blizzard - which is worth around $13.4bn – is reportedly part of the firm's ambitions to rectify the situation.
Activision Blizzard has reportedly been offered to major games companies including Microsoft and Tencent, but a share buy-back from Activision Blizzard itself is also apparently under consideration.


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While having dinner last Wednesday, I inquired slyly as to whether the gravy was a bit more salty. And there came the reply from my wife, "Is it?", with a grin.
The reply was a perplexing one as the it didn't quite answer my question and I still had to figure out the answer.
The incident was forgotten as the burden of my new job took toll until the weekend. Weekends are a great time and I thought I would help my wife in the kitchen. For some reason, during the preparation, I was asked to add the salt and the dish turned out to be saltier than it should have been. I have never been a cook except for an occasional omelette, boiled eggs, tea and coffee.
The same weekend, I had the honour of adding salt into another dish while assisting my wife and luckily enough it was just the right amount of salt.
I now wonder whether my wife intentionally asked me to help her out to add the salt to the dishes. Well, I had learnt a wonderful lesson and got a good story for my blog.
Moral of the story, "Add only a pinch of salt to prepare your dinner."
This is my first experiment and public interface with the WWW, basically intended to understand and explore the so called "BLOG". A late entrant into the field of blogging, I would like to study the nature of blogs, the responses, the reach of the WWW and any interseting aspect of the WWW, if possible by statistical means. The Blog would then later blossom into sharing my views and thoughts on random subjects and I hope to elicit amicable, healthy, nonoffensive comments from my Dear Readers.
Saturday, December 02, 2006
1 comment:
- VillageLad said...
Hmm it's all nice to hear that you can cook :P, So when you are throwing a party
Subscribe to: Post Comments (Atom)


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Recently, we showed you behind the scenes footage from Real Madrid 's locker room. From that pre-season video , we saw head coach Zinedine Zidane shake each players' hand as they walked through the entrance.
When asked why he does the ritual before and after every game, Zidane shrugged and simply replied: "It's just something we do."
Proving it wasn't just a one-off for the cameras, Madrid have posted another video (which you can watch above) of the Frenchman carrying out his much loved tradition.
But instead of focusing on Zidane, this time we're looking at the players - and their handshakes tell us a lot.
Here are just three we picked out from the from the video above:
He's cool and smooth.
Marcelo opts for a short and sweet fist-bumping hug.
He's quite a shy guy.
Gareth Bale goes for the traditional handshake, while looking incredibly uncomfortable.
He's a lover of the game.
Sergio Ramos plants a kiss on Zidane's cheek.
Each player that encounters Zidane has a different handshake, and every one tells us something small about that player.


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Cumnor House
A fantastic, friendly, local school that pings its cheery pupils off to more than 20 top seniors around the country. Learning to climb trees, fall down and get up again is considered as core as ABCs, with most children begging to board come Year 7 so they don't miss out on 'the best fun ever'. The vibe is informal, and the children don't wear ties with their pillar-box-red blazers. Yet all pupils, as one mother points out, 'can (and do) look an adult in the eye and shake hands politely without being prompted'. The school had a healthy crop of scholarships to report in 2015 (26); boarding numbers are rising like beanstalks; and no sooner was Cumnor's new nursery opened than it had to be expanded. Staunch community spirit and plenty of parental activity are encouraged: weekly dads' football is a must, and the coffee and croissants at morning drop-off are a daily parental godsend.
- Location
- Danehill, Haywards Heath, West Sussex RH17 7HT
- Head
- Christian Heinrich
- Pupils
- 390 day and boarding: 211 boys; 179 girls
- Faith
- C of E
- Ages
- 42807
- Term fees
- Day: £6,265; boarding: £7,455
- Website
- cumnor.co.uk


text:
Review by Cat Metayer
Anthony Morton's paintings stand out in a beige and brown office setting; their vibrant colors and contrasts creating an appreciated focal point for an art school career workplace. Morton utilizes college, internet, and popular culture for his "Miss Series;" a collection of paintings based on pictures taken from popular networking site, myspace.com.
Morton, a sophomore at Pratt Institute, works primarily with acrylics for the series, with a combination of realism and imagination. The paintings themselves are highly stylized interpretations of the photographs, with colorful embellishments; whimsical, cartoon inspired shapes, adding a touch of fantastic to the portraits. The colors of the flourishes are reminiscent of the 80's, a decade widely mined by the modern youth culture. The largest painting, titled "Miss Ali S." is Morton's first experiment with oil based paints and perhaps his most resolved.
The photographs used for his work are the images which these women chose to display themselves to the internet populace, a fact that Morton bases the series on. Attention is paid not only to the subjects, but also to their chosen attire; their designer sunglasses, necklaces, and earrings, giving the consideration to the items which these characters use to build their image.
The popular "myspace pic" the very best image, according to the subject, based on lighting, angle, and fashionable attire, is a mainstay for modern youth culture. Some say this gives the subject a feeling of power over their image in the world, while others claim that it is a deceit of the internet public. Morton's work seems to speak to the former, further stylizing an image deemed by the subject as the best depiction of themselves.
October 13th-November 4th, 2008


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Mori Dress Tour
I'm baaaack! Haha, I've been keeping up with my Instagram feed, but I'm so behind in reading blogs let's not even talk about how behind I am at WRITING a blog. I just had to pop in to share my Mori dress with you! I started eyeing this pattern when it first came out, so when Heidi from Elegance and Elephants asked me to join in the pattern tour, I couldn't say no! Besides, having a deadline is the only way I'm getting any sewing done these days!


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Khursheed demands govt to pay compensation to Gadani incident victims
ISLAMABAD: Opposition Leader Syed Khursheed Ahmed Shah has demanded the federal government to give compensation to the victims of Gadani ship fire tragedy, ARY News reported.
At least 20 people were killed and over 22 others were wounded in the blaze after blasts ripped through an oil tanker at a ship-breaking yard in Gadani.
Khursheed Shah, in his letter to Prime Minister Nawaz Sharif, has stated that at least 26 labourers lost their lives in the incident whereas several others were injured and many others went missing.
The opposition leader said that the incident was very serious in its nature.
He demanded the government to pay compensation to the victims of the fire and take action against those having involvement in the fatal incident.
It should be noted that the labourers in the ship breaking industry often work in poor safety conditions without basic protective gear.
The Gadani ship-breaking industry has fallen on hard times recently and employs about 9,000 workers, fewer than in its boom years at the end of the last decade.


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Folk music and politics have long been intertwined in American history, and some of the most powerful statements for social change have come out of the folk song tradition.
A classic example is Woody Guthrie's "This Land is Your Land," which many of us learned and committed to memory grade school.
Less known is the fact that he originally titled the piece "God Blessed America" when he wrote in 1940 in reaction to Irving Berlin's "God Bless America." It was a disdainful commentary on the social injustice Guthrie saw across this country.
Guthrie's view was put forth forcefully in some of his original lyrics for the song, which included:
"There was a big high wall there that tried to stop me. The sign was painted, said 'Private Property.' But on the backside, it didn't say nothing. This land was made for you and me.
One bright sunny morning in the shadow of the steeple, by the relief office I saw my people. As they stood hungry, I stood there wondering if God blessed America for me."
We found similar passion for righting social injustice and promoting income equality in Bernie Sander's campaign for President. That is why we decided to write and dedicate a song in the folk tradition to the movement that he inspired.
"The Song That Needs to be Sung" is a music/video tribute to the protest songs that came before and to the ones that are yet to come. It is a call to action, and also an acknowledgement of the great achievements Bernie's campaign has made in fighting for a progressive economic agenda in America… so that one day, we can really say, "This land is our land."
H/T DailyKos


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Unverified Voracity Puts Its Head Down
Unverified Voracity Puts Its Head Down
UFR coming fiveish. Sorry for the delay.
Bo + DKR + Keith Jackson. Self-recommending, from before the 2005 Rose Bowl:
HT: MGoVideo
The Trouba hit. Here it is:
Keep your head up. I guess that's a point of emphasis and stuff, but a few years ago that's just a bad spot to put yourself in.
Michigan takes on State this weekend; Yost Built previews. Rutledge gets the start tonight; Trouba and Serville return to the lineup.
Predictorama. Blogs say things!
- Who Are You And Why Do We Care predicts Michigan 31-17 in unnecessarily tiny font. Fouad agrees, as does The Big House Blog.
- The MZone goes with 31-24, Michigan.
- TTB says 38-20, Michigan.
- Maize and Go Blue says 28-17, Michigan.
- Northwestern's Scout guy says 31-23, Michigan.
- BWS goes with 20-17, M.
- Maize and Brew says 20-18, which is like a score I would predict.
- Maize and Blue Nation goes with 32-13.
- Tremendous goes 30-23.
- Three of four SOP folk predict a Michigan win with one holdout who always picks Northwestern to win and one guy talking soccer.
Talkin' Wildcats. I do the Q&A thing at Lake The Posts…
LTP: Finish this sentence. Northwestern wins on Saturday if they prevent __________ from ___________
MGB: Michigan's linebackers from flowing to the ball. Given the pass offense NW has–Nebraska is NU this year, remember–if Northwestern is going to put up enough points to win it'll have to be on the ground, and Michigan's linebackers are flowing clean thanks to a bunch of Mattison slants. Get to them and you've got a chance.
…and Sippin' On Purple:
Northwestern is "ranked". Michigan "isn't." Should they be? Do you like the fact that you played two still-undefeated teams in non-con play?
Mgo: No. No Big Ten teams should be ranked, and in fact anyone who votes for a Big Ten team should be put under house arrest. Indiana may go to the Big Ten championship game. QED.
I like playing ND. Check that: love playing ND. I hate those guys. I love going to Notre Dame Stadium, and am immensely depressed the series is coming to an end so ND can play Stanford for some reason. And Purdue! Come on man. They're good this year, but that series is a bedrock of my Michigan fandom.
Playing Alabama in Dallas sounded like a good idea when it was scheduled. By the time the game rolled around it was clear that it was not.
Denard in the NFL. Rotoworld takes an interesting look at the kind of things Denard might be able to do in the NFL:
When I watch Robinson run (and the way Michigan uses him), I can't help but think his best role would be to start out as a returner and as a situational running back. He's listed at just 6'0/197 pounds, but has a thickly built lower body. The Michigan offense has taught him to make the same types of reads NFL runningbacks are taught. He's totaled over 200 carries the last two seasons, and he was the entire foundation of their run game in 2010 - totaling 256 attempts for 1702 yards (6.6 YPC) and 14 touchdowns. He has a tendency to get upright, but he's a tough and patient inside runner who reads and sets his blocks up well, hits cutback lanes with authority, and has the speed to do damage in space. …
Highlighted in green, the playside linebacker moves Michigan's tight end into the backfield. The strong safety has filled hard off the edge, and the two inside linebackers are scraping hard playside. Instead of allowing the defense to string the play out, Robinson takes advantage of Alabama's pursuit, planting his outside foot, re-directing, and hitting the crease swiftly.
Denard gets skinny through the trash in the cutback lane. He leans and squares his shoulders through the tackle, which allows him to pick up an additional three yards.
Much more at the link.
More Ferns. Tom Rinaldi is just warming up for this guy:
They're not moving it. Dave Brandon will not let the whole playing at Rentschler Field thing die:
"We continue to have discussions with UConn to see if there isn't a better plan," Brandon told a small group of reporters Friday shortly after speaking at the Michigan Sport Business Conference at the Ross School of Business. "Anything's possible.
"It's totally in their control. We're trying to provide them with as much persuasion as we can, in the form of financial benefits for them to move that game to a bigger stadium."
This is not happening. The Connecticut legislature put a bunch of money into that stadium and it is now losing more money than projected; they will freak out if the Michigan game, a guaranteed sellout, is moved.
Michigan is paying BYU and Colorado 1.3 million to come out, BTW. The gap between that and 900k, 1 million dollar snackycakes is incredibly small.
Wait what? I missed this when it happened, but apparently the Orange Bowl is now going to be a potential destination for Big Ten teams that miss out on the Rose:
The Orange Bowl matchup in the new playoff era has been finalized multiple sources told CBSSports.com on Wednesday.
The ACC champion will play the highest-ranked team among Notre Dame and available teams from the SEC and Big Ten beginning after the 2014 season.
This is a problem for the Pac-12 and Big 12, who want access to more than one big bowl but the problem…
"TV doesn't want it," said one official intimately involved in the process.
So whatever.
Etc.: Jeopardy does CFB names. Lloyd Brady profiled by ESPN. "Deal with it Jesus" is amazing. Stubhub wins over season tickets this game; by end of year it will be a better deal, I imagine. A report from an alternate universe. Slippery Rock preview. Delany talks big about Big Ten scheduling, but which he means Ohio State scheduling. And MSU, I guess. Dump these divisions already.
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With Father's Day coming up, the THANN team has been working hard in store to get ready for this special day. We took a break from THANN Skin Care's hustle and bustles today to chat—sans business—about our DADs. Fadi, THANN Canada President and soon-to-be-father, shared one of his father's wisest words for him growing up.
"My dad always emphasized the importance of school. EDUCATION was the #1 priority because he believed that that would give my siblings and I a good…no, GREAT future," Fadi says.
He adds, "There were not many restrictions growing up as long as we did well in school. We were allowed to do whatever we wanted, go wherever we wanted to on the condition that we did not lose focus in school."
Judging from Fadi's many achievements, which now include being named one of Ottawa BusinessJournal's Top 40 Under 40 winner, his dad's wise words were worth listening too!
|Fadi shaving for his dad. What a wonderful son!
Now, here are some of Fadi' s wise words about Father's Day gift ideas…because men NEED pampering too:
- Aromatic Wood shower gel
Because it cleans thoroughly for healthy skin. Plus, the scent of orange, nutmeg and tangerine oils in this shower gel will leave him feeling energized and smelling great.
- Sea Foam shampoo
Because the mint oil in this shampoo refreshes the mind and spirit while fighting dandruff away.
- Rice Extract Face Cream
Because everyone needs an effective non-greasy moisturizer! It helps prevent and protect dry skin providing temporary relief from scaling and chapping associated with dry skin.
- Shiso Astringent Toner
Because it can be used as an alternative to his aftershave. He'll be saying goodbye to burning, stinging sensations and saying hello to a cool feeling after shaving with THANN's all-natural, alcohol-free Shiso Toner.
- Rice Extract Lip Balm
Because it nourishes and moisturizes the lips all-year round. Who in their right mind welcome chapped lips? No one. Thought so.
What do you think of Fadi's Top 5 picks? We'd love to hear from YOU!
HAPPY FATHER'S DAY from THANN!
Follow THANN on Twitter : @THANNSKINCARELike THANN on Facebook: THANN


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from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. Plural form of ha'penny.
Sorry, no etymologies found.
"I'd have to give you ha'pence change, Brigadier," he replied, mendaciously.
"That, and ha'pence will buy thee a wheaten loaf," he said dismissively.
They were copper, they did feature the Queen's profile, and they were the size of a ha'pence.
He'd nothing to lose except a thousand quid, and that was ha'pence to him.
God knew I hadn't cheated -- when I cheat, it's for something that matters, not sweets and ha'pence.
To play such a part often earns, in an old saying "more kicks than ha'pence".
Ulster's Place In Britain Today/The Economic Future of Great Britain
He took the boat across the river, despite the additional three ha'pence that it cost, first telling himself that he had to go to the post office to hand in his letter to Maria, and then amusedly admitting that it was a temptation to find himself afloat again after three days on land.
It was not always blue, he found; the ship was not always running before a spanking breeze; more kicks than ha'pence, more rope's-endings than blessings, came his way during the first few years of his sailor life.
The printer actually has to interpret in his printing the etcher's meaning, for the which, as a rule, he gets "more kicks than ha'pence," and in the end wishes he had stuck to plain plate printing as far as the profit is concerned.
The Building of a Book A Series of Practical Articles Written by Experts in the Various Departments of Book Making and Distributing
Once for a tight little Island, fonder of ha'pence than kicks,