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This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PBX1, Human
PrimePCR™ Template for Probe Assay: PBX1, Human
Assay Information
Gene Information
This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis and required for skeletal patterning and programming. A chromosomal translocation t(1;19) involving this gene and TCF3/E2A gene is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2011]
Products used to generate validation data:
Summary Data:
Amplification Plot
Amplification of cDNA generated from universal RNA.
Melt Peak
Melt curve analysis of above amplification.
Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.


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Charlotte County schools say more guns in classrooms isn't the answer
"I think anything to protect our children, I would go for it," said concerned parent Angela Rossetti.
But for Charlotte County Schools, more guns outside of trained law enforcement isn't the answer.
"It's not an active shooter problem, it's a mental health problem," said District Spokesman Mike Riley.
Riley says that despite Gov. Scott's signing a new school safety bill in response to the Parkland shooting, only school resource officers will be allowed to have guns on campus.
"When you look at a law enforcement officer, they're highly trained. When you look at a SWAT team who goes to answer these things, they're more trained. I mean our teachers are trained to be teachers," Riley said.
The School Guardian Provision of the bill sets aside money to train and arm certain school staff members if the local sheriff's office and district allows it.
"I know nothing has happened yet, but that doesn't mean something won't happen," Rossetti said.
The bill also helps fund "hardening measures" like metal detectors and bullet-proof glass and doors. But the district says the money they'll get isn't enough.
"You can't turn schools into bunkers," Riley said. "$400 million probably wouldn't even do Dade County, let alone the other 66 counties."
Instead, the district will use their share to hire more school psychologists and social workers and add additional school resource officers to high schools and middle schools.
"Reaching them at an early age and helping the children that are troubled and need the help, that's where the answer lies," Riley said.
Though some parents feel more should be done.
"Several times I've had calls from the school that there's been a bomb threat, or there's been a gun threat and it's scary as a parent thinking anything could happen to my child at any time in school," Rossetti said. "So it is worrisome these days."

subset: commoncrawl


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As Salaamu Aalaykum,
ICAN is having a new class for those interested. http://ican-online.org/none/ican-birth-c...prevention
NEW Online Webinar
Monday, July 27 - 9:00 pm EDT (6:00 pm PDT)
Planning YOUR birth?
Are you keeping your fingers crossed and hoping for the best? Well, take charge and find out ways you can increase your chances of having an easier and safer birth. First-timers and experienced mothers both can benefit from this class.
This 2-hour online session will help you learn:
* Different kinds of care providers you can use, and the pros and cons of each
* How to empower yourself to make educated choices during your pregnancy and during labor
* What factors contribute to your chances of having an unnecessary or preventable cesarean
* What is the "downward spiral of intervention"
* Why avoiding an unnecessary cesarean is safest for you and your baby
* When cesareans are truly necessary
Monday, July 27 - 9:00 pm EDT (6:00 pm PDT)
$20.00 fee benefits ICAN - click here to register.
Frequently Asked Questions
What is a webinar?
Webinar is short for "web seminar." These online seminars allow many participants in multiple locations to see and hear a presentation from their own desks. To attend the ICAN webinar, you will need a computer with internet access to connect to the presentation, and a phone line OR speakers and microphone to connect to the audio. A webinar typically consists of a moderator, a presenter and an audience.
I have small children. Will I need to be in a quiet room to attend this webinar?
No. To maximize audio quality, all webinar attendees are automatically muted. If you have a question, you will have the option to be temporarily unmuted or type in your question through the Questions Box on the Webinar interface.
I filled out the registration form, but I was not re-directed to the ICAN store. How do I submit my payment?
To submit your payment, please click here, click "add to cart" and proceed to checkout. When prompted, choose "Pay now with Paypal." You will have the option to log in to your Paypal account or to pay by credit or debit card without logging in.
What is your refund policy?
The registration fee is fully refundable if cancellation is made in writing by Sunday, July 26 at 9 pm EDT. No refunds will be granted within 24 hours of the start of the webinar.
Presenting ICAN's official cesarean prevention class is Claudia Villeneueve, President of ICAN Canada, ICAN's former Education Director, and a birth doula and childbirth educator. Claudia is also an engineer and lives in Canada with her husband and three children. Her first birth was a cesarean for failed routine induction, listed in her medical records as "failure to progress." Her second birth was a completely natural, drug-free and inspiring VBAC. Her third birth was a cesarean for complications with the epidural.


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If you speak from the heart and with thoughtful compassion you shouldn't have to censor your voice but that's not always the case. Best conversation are fluid. ~ Jessica Ord
Why people feel they have to be someone else. Being yourself is the best thing. Lies will be discovered & all that does is give you extra stress. ~ Tony Horta
Love being with someone I can trust to be myself. ~ Julie Goldberg
Those relationships do exist. Some times life just cuts them short. So live each day like its your last, never have any regrets, and know you are loved. ~ Brenda Ramey
I love being me, and it's a pleasure when a person can accept me & respect me for the person that God created me to be. ~ Sharon Polk
I usually have these conversations with myself. ~ Anne Bentley
God is the best to talk to. He loves you no matter what. He doesn't judge you. He is our best friend. He loves us unconditionaly. ~ Vicky Kelly


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Mother-in-Law Cake (Applesauce Cake) Recipe
allspice, cinnamon, cloves, +flour, +applesauce, +baking soda, +cocoa powder, +dates, +eggs, +nutmeg, +raisins, +salt, +sugar, +shortening, +walnut
www.cdkitchen.com - Similiar recipes
Carrot-Spice Cake (Or Apple-Spice Cake)
allspice, cloves, cinnamon, +buttermilk, +maple syrup, +almond, +vegetable oil, +raisins, +carrot, +flour, +eggs, +ginger, +nutmeg, +salt, +baking soda, +vanilla extract
www.cooks.com - Similiar recipes
Crusader Cake
A wonderful spicey cake with a delicious lemon glaze. Starts with a cake mix, but you can't even tell. As my D-I-L would say this cake is "wicked...
allspice, cinnamon, cloves, +beer, +eggs, +ginger, +cardamom, +vegetable oil, +vanilla instant pudding mix, +cake mix
Beer Spice Cake
"A quick and easy spice cake made with beer! Our family enjoys this cake as a Christmas Eve goodie!"
allspice, cinnamon, cloves, +baking powder, +baking soda, +beer, +brown sugar, +butter, +eggs, +flour, +salt
Christmas Cake
"This cake is a rich, dark, moist fruit cake, very flavorful at Christmas. Try icing with almond paste for a more festive touch. This recipe is started...
allspice, cinnamon, cloves, +apple juice, +baking soda, +brandy, +brown sugar, +butter, +dates, +currant, +eggs, +flour, +molasses, +raisins, +salt
Spiced Yogurt Pound Cake
"This is a rich, moist and delicious spiced pound cake I have had since 1974 and always make it during fall and winter months. The cake needs no frosting...
allspice, cinnamon, cloves, +baking soda, +butter, +eggs, +flour, +nutmeg, +yogurt, +salt, +vanilla extract, +sugar
Groom?s Cake
The groom?s cake is a dark, moist fruitcake that is steamed. It may be made months in advance and frozen. It is presented unfrosted and golden brown,...
cinnamon, allspice, cloves, +flour, +almond, +butter, +citron, +powdered sugar, +eggs, +grape juice, +lemon juice, +lemon zest, +nutmeg, +raisins
Beer and Date Coffee Cake
I thought this cake was very good, but then, I'm a date-lover. The cake is spicy, though not overpowering in my opinion. The texture of the cake is fairly...
allspice, cinnamon, cloves, +flour, +baking soda, +brown sugar, +butter, +beer, +dates, +eggs, +pecan, +powdered sugar, +salt
Eggless, Butterless, Milkless Cake Recipe
cinnamon, allspice, cloves, +lard, +brown sugar, +raisins, +water, +salt
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Sour Cream Spice Cake
"Edna Hoffman, Hebron, Indiana, perks up a meal with this old-fashioned layer cake. The dense cake is rich in spices, while the frosting is fluffy and...
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GOP Rep: Obama Administration's OSHA Targeting Non-Union Plants with Inspectors Accompanied by Union Reps
On Wednesday's "On the Record" on the Fox News Channel, Rep. Martha Roby (R-AL) laid out an argument that this administration is using the inspection powers of the Occupational Safety and Health Administration (OSHA) to target non-union plants in South at far more disproportionate rate than anywhere else in the country. She added that also these OSHA inspectors were being accompanied by union representatives to non-union shops.
"Well, it came to light for us when we noticed that there were more and more stories being reported around the country where OSHA was designating union representatives to come along for inspections in non-unionized industry," Roby said. "At the same time, OSHA issued what's regional emphasis program that targeted the automotive parts suppliers in Alabama, Georgia and Mississippi. And they did so without justification. So, our problem is, number one, we're questioning whether or not this administration's interpretation of the law affords OSHA the unilateral opportunity to bring along a union rep to non-unionized corporation for an inspection. But, also, to target specific industry in a specific part of the country without offering the appropriate justification."
Click here to read this article on the publication's website.


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Ultimately, after obtaining a patent (or patent-pending) every founder reaches a crossroads where they will have to decide methods to proceed with commercializing their technology. Do they develop, manufacture and recent market the product on the their own? Also do they try partnering/licensing with a company who will likely then bear significant of the income risks of event? Whatever the case, after the innovation has been lined with a patent, the inventor will need to weigh these options and decide those one is the entire right choice to produce his or your partner specific situation.
If you will definitely be new to ones business of inventing or are not really sure on what an inventor certainly makes money caused from an invention, take into consideration the following ways.
Licensing that this invention to get royalties:
A license agreement is just when one particular inventor [licensor] agrees within order to let a very third party [licensee] commercially use or even invention over a cycle of moments. Typically, inventor would receive either a an current payment branded a "royalty", (calculated compared to a chance of product sales of your invention) or alternatively a one-time lump-sum pay. The company/licensee normally proceeds with developing, manufacturing and in addition marketing usually the invention, which allows most of the inventor to shift these kind of cost and even risks for you to the licensee. Also to licensing, the inventor may rely of the expertise of the experience moreover established smaller business to lift the device's chance out of success.
Assigning and for Selling i would say the Invention:
When the entire inventor assigns his rights, he could be described as permanently uploading or moving ownership using the invention/patent. The inventor may receive a swelling sum paying or each series related payments. The difference through a "license" and "assignment" is doing the monitor of rights. With the new license, unquestionably the inventor most likely will retain evident rights (like "renting" each of our patent), additionally with an assignment companies transfer their whole rights (i.e., sell it).
Developing & Manufacturing each of our Invention:
Typically, company with high hopes of eliminate their products into that business even they would expect to sell his product might possibly be the best runners for manufacturing. Manufacturing and marketing good invention may be a superb exciting and rewarding decide on for a number inventors still the activity should are more looked by visiting more as a web business venture, while it demands the designer to offer substantial bucks and a very well thought out consider on how to develop, manufacture in addition to market your idea. Manufacture is very different than finding that you simply company to allow them to license each of our invention, and should not be dived cowardlessly into with out examining the particular risks combined with carefully creating the best route to produce success. how to file a patent
If you can choose some of the path towards developing and even manufacturing your entire invention high on your own, you preserve far a whole lot control linked to your creation. But you may also are convinced the completely different risks while costs associated, such as: research, engineering, tooling, molds, inventory, warehousing, freight expense, distribution, and more. You also assume the very investment akin to your rather own time, that will for almost all inventors that hold full-time jobs, is simply difficult.
For a handful of inventions, little development in addition to the setup is simply required, which probably can shorten the the manufacturing field process. Other, more complex inventions might possibly require long more cost. Minimum order requirements should certainly also there inventors to a challenge, both momentarily and in terms associated with storage web space. For any inventor who finds any of these aspects of manufacturing to make sure you be as well costly, too difficult or too considerable of a new hassle, finding a licensing agreement could be a meaningful more fitted solution. InventHelp Product Development
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It is always important at remember that experts claim while regulations licensing regulations manufacturing should be a for sure success, taking the season to grasp the choix for bringing your development to sell and opinion through the text your objectives and goals are last to diving into either option would help the public take some path which happens to be right for you and thus your formulation as you and your family continue step the route of inventing.


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Eroga'tion. n.s. [erogatio, Latin.] The act of giving or bestowing; distribution.
No Sources Quoted (639)
Latin (690) · Noun Substantive (1269)
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Cite this page: Johnson, Samuel. "Erogation." A Dictionary of the English Language: A Digital Edition of the 1755 Classic by Samuel Johnson. Edited by Brandi Besalke. Last modified: February 1, 2014. https://johnsonsdictionaryonline.com/erogation/.
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Delano K. Pruner, age 85, of Waxahachie, passed away Tuesday evening, August 14, 2018, at Baylor University Medical Center in Dallas. He was born November 8, 1932, in Kansas to George and Reba (Etchinson) Pruner. He was a Veteran of the U. S. Air Force.
Survivors include his loving wife, Mary Pruner of Waxahachie; four children, George Pruner and his wife Janice of Abbott, TX., Cindy Johnson and her husband Terry and Jim Pruner and his wife Jennifer all of Waxahachie and Mona Gatens and her husband John of Dallas; step-children, Greg Calder of Austin, Jennifer Calder of Midlothian; 10 grandchildren, 12 great grandchildren, 1 great-great grandson and numerous nieces and nephews. Delano was preceded in death by his parents; two brothers, JR Pruner and Darrell Pruner and one sister, Beverly Lair.
Visitation for family and friends will be 6 to 8 PM Friday, August 17, 2018, at the Boze-Mitchell-McKibbin Funeral Home.
Funeral services are scheduled for 2 PM Saturday, August 18, 2018, in the chapel of the Boze-Mitchell-McKibbin Funeral Home with Randy Houte officiating. Interment will follow at the Red Oak Cemetery.
Pallbearers will be grandsons, Nick Pruner, Alex Pruner and T. J. Johnson and nephews, Johnny Pruner, Randy Cubbage and Scott Burkhart. Honorary pallbearers will be Midway Airport co-workers, John, Ray, Darron, Paul Fletcher and Randy Pike.
To view an obituary or sign the guest book, please visit www.bozemitchellmckibbin.com.
Arrangements are under the direction of the Boze-Mitchell-McKibbin Funeral Home 511 W. Main St., Waxahachie, Texas 75165.


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Random Reviews of Culture Stuff Part 1: Welcome to Oblivion
In 2009, my favorite living author was Doris Lessing. Margaret Atwood, Gabriel García Márquez, and Toni Morrison were also on the list in no particular order after Lessing. Now Lessing and Garcia Marquez are gone. Morrison and Atwood are still going strong, especially with the Hulu TV adaptation of Atwood's chilling 1985 novel The Handmaid's Tale busting up the charts. And deservedly so. A review of this series is in the works. But at the time, I was thrilled to discover another one for the short list (which is an oh-so-clever reference to a Lessing short story title), but it turned out I was wrong. Very disappointing.
I read a collection of stories by David Foster Wallace (DFW) called Oblivion, and I was seriously blown away. I went to post something about him on Facebook, and in seeking a link, I found, not surprisingly, what appeared to be his website, http://www.davidfosterwallace.com, although just beneath the website title it said, "this [sic] website is unofficial, as are you." Hard to argue with that.
I am hardly the first person to discover DFW. He is a well known author of some critically acclaimed short stories, a massive novel called Infinite Jest, and some other books you can look up for yourself (I don't mean that to be snarky, just lazy). He'd been on the scene for decades; I just had never read him. I'm kind of out of touch since I stopped reading newspapers some years ago and don't get the Sunday literary supplements anymore. But this way I "discover" writers all my own, without bias.
I came across his work while browsing in the historic and brilliant City Lights bookstore in San Francisco. I have spent hours in this wonderful store, and there is almost nothing in it I do not lust to read. However, budgets and storage being what they were (paltry), I limited myself to purchasing one book, two if I was feeling flush and the second book was really, really cool. However, to assuage my lust, I would jot down book titles and authors so I could check them out of the library, for free, without committing myself to any home shelf space.
Which is how I came across DFW, browsing in City Lights. I liked the name Infinite Jest, derived as it is from one of everyone's favorite literary interludes, Hamlet's famous graveside musings about poor Yorick, a fellow of infinite jest. Because when he was alive, Yorick was a jester. So, you know, that's good. DFW must seriously dig Hamlet, since not only is his novel title from Hamlet, one of the stories in the collection is also Hamlet-based, 'Philosophy and the Mirror of Nature,' an extremely strange tale about an arachnophiliac and his plastic-surgery-monster mother riding the bus. But then, what writer doesn't dig Hamlet. And the story is, I think, really about the nature of fear and how we deal with it, culturally and individually.
Anyway, I jotted down DFW's name and the title Infinite Jest. On my next trip to the library, I found that my local branch didn't have Infinite Jest, but it did have a collection of DFW's stories called Oblivion. So I checked it out. And boy oh boy, were they good. Not easy reading, but definitely compelling and absorbing reading. This guy was the real deal. He got a place on my bookshelf for sure. I spent most of the following days reading him or writing about him.
The stories are so recent and the tone so contemporary that I assumed he was fairly young (a term that grows more inclusive as I get older) and that he was still writing. Well, yes and no. If you clicked the link above you'll already know this, but I didn't until I found the link.
He was indeed young, younger than I was, but he was not still writing, because he was dead. Fairly recently dead. By his own hand. Which is a damned shame and kind of pisses me off because now there will be no new DFW works to read. (I dreaded Lessing's passing for the same reason.) Fortunately for me, I had just started reading him and so I had quite a few books to go. I still do.
What's weird is that I felt compelled to write about DFW just after finishing the fifth story in the book, about a guy who kills himself because he knows himself to be a complete fraud, although the knowledge of his fraudulence doesn't help him overcome it; in fact, his insight into his own fraudulence only seems to enable him to be an ever more skillful fraud, so he sees no escape from perpetual fraudulence in this lifetime. He tries a lot of different ways of finding authenticity and being genuine, to no avail. The narrative voice of the story is, well, it's sort of transtemporal, as it were.
It was this story that galvanized me into seeking DFW on the web, before I'd even finished the collection. I still had three stories to go, including the title story, but so far all the stories had been so good, so original, full of amazing utterances and insights, the kind that you recognize instantly as true while still being utterly fresh. You think to yourself, "Yes, that's exactly right, I just hadn't taken the trouble to think it through that clearly and completely." Well, DFW did, although I'm not sure that it did him much good.
He was only in his mid-40s when he died. Here's what the unofficial website had to say about him:
"David Foster Wallace is the award winning author of several novels, more than a few short stories, and numerous articles, as well as being a college professor. [While] still in his 30's, David has been called one of America's most important young authors and is often compared to Thomas Pynchon, though he tends to shrug off that association. He is most widely known for his epic (1000+ page) novel, Infinite Jest, published in 1996 and critically acclaimed by critics and readers alike. Topics covered in Wallace's work are wide ranging, but he seems to have a special interest in American culture, addictions, and excess. Ironically, because of his edgy body of work and his public persona, DFW has gained a cult following and become somewhat of a celebrity himself."
I personally find him much more readable than Pynchon, whom I never liked all that much, not because he's not good, but simply as a matter of personal taste. And readability. I mean, really, has anyone ever actually read Gravity's Rainbow all the way through and lived to tell the tale? I know there are a few daring souls out there who have, but I am not among them. I never finished Ulysses either. Bad English major, bad! They used to have a read-a-thon of Gravity's Rainbow, Ulysses, and Finnegan's Wake at my university when I was there, but I never attended. This is how English majors had fun in my day. Well, not me, but some of them.
All the DFW stories I've read so far are excellent, even brilliant. They all have to do with the inner workings of people and how they operate in their cultural settings; the contrast between what the outside world sees, expects, and demands, and what is really going on inside; the vast malleability and manipulability of people and the simultaneous difficulty of accessing (by others or by themselves) their real core self, all told with exquisitely honed acumen and craft.
One of the stories was too heartbreaking and horrible for me to finish, and it's only three or four pages long. It's called 'Incarnations of Burned Children,' and while it is superbly crafted and emotionally riveting, it is about what the title indicates, and I could not finish it. Especially not recommended for parents.
He had me hooked by the middle of the first story, which bears the wonderful title of 'Mr. Squishy.' It is about market research and corporate/sexual/power politics as sort of metaphors for how we live and as means of alienating us from our own lives and selves. Since I used to do market research for a living, it definitely piqued my interest, although my involvement in the trade was pretty fringe and niche, while this is very hardcore, almost pornographic in its dissection of people's desires and maneuverings and the perpetual quest to reduce people to formulas by people who paradoxically fondly believe themselves to be unfathomable and indefinable. There's a fascinating element to this story that I still have not figured out involving a human fly climbing the building in which the action is taking place. If anyone has read this and has some ideas, please let me know.
The other story that really grabbed me was 'Another Pioneer,' which got to me because of my religious identification ( as opposed to my former occupational identity). It is essentially a parable about religion, which in itself is sort of abstract, since religion itself uses parables as a way of explaining its themes. It takes place in a quote-unquote primitive village in a recognizable but undefined location, possibly remote Africa, and uses that self-contained setting as a way of putting the vast and cosmic into a human-scale setting. The meta-setting, as it were — the place in which the story is actually narrated — is a vivid contrast to the quote-unquote primitive locale of the story being told in the story. As it were. In a way, it's a very Bahá'í perspective. But then, I would see it that way, wouldn't I.
I never finished reviewing all the stories, since it was a library book and I had to give it back, and then life got weird and I ended up in Honduras (long story). But they have since made a movie about DFW called The End of the Tour, starring Jason Segel as DFW. It was pretty good, but I am really impressed with the fact that it got made at all. I saw it in an airplane, which was sort of fitting, since it relates perfectly to the meta-setting of 'Another Pioneer.' I was the only person on the plane watching this particular movie (I checked). Everyone else was watching movies with lots of explosions or superhero sagas, which is all anyone seems to watch these days (don't get me started on Logan, maybe some other time), and I'm watching a movie about a series of interviews with a dead author. It was, in fact, on the way to Honduras, now that I think of it. This is somehow emblematic of something, very meta. Too bad DFW isn't around anymore, I think he could make something of it.


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Fundraising campaigns posted on TrustLeaf arent public; theyre visible only to people the campaign creator invites by email. Thats actually one of the key differentiators of our site, Liang says. A lot of people want to keep their business idea and their fundraising process private, especially when asking friends and family for money. Theres no minimum ask for fundraising campaigns on the site, which tend to span 30 to 45 days. Borrowers can invite as many supporters to contribute to their campaigns as they want. They can also suggest several potential loan amounts and repayment scenarios. Attorney Curtis Mo, a ขาย ตรง เปิด ใหม่ ล่าสุด partner at global law firm DLA Piper and a TrustLeaf board member, created the sites template loan agreement. Entrepreneurs plug in the names, loan amount, termsand interest rate, and then both lender and borrower sign the document online, where its accessible 24/7. No private financial data is required to create a campaign or contract on the site. Borrowers can use TrustLeafs tracking and communication tools to keep tabs on payments and update lenders on their businesss progress. The ability to accept loans and repay borrowers through the site is coming, Liang says; the same goes for a convertible equity note template founders can use for loved ones who would rather be repaid in company shares than interest. The formal contract and ease of use made believers out of Meisch and his family.
For the original version including any supplementary images or video, visit https://www.yahoo.com/news/2-services-help-friends-family-200000246.html
The formal contract and ease of use made believers out of Meisch and his family. They made the process organized and added a sense of legitimacy to what I was doing, he says, instead of me just picking up the phone and asking, Hey, can you loan me another $1,000? Armed with the additional funds, Meisch was able to stay in business long enough to attract the attention of Walter Reed National Military Medical Center, which has conducted trials with his product on PTSD patients. Able: This company will give you a loan -- but theres a catch When the opportunity to buy an established hair salon fell into Hayley Grolls lap, she quickly took stock of her financing options. The veteran hairstylist wasnt approved by the online lender she initially contacted. Then she found Austin, Texas-based Able , which bills itself as a collaborative lender. Within three weeks, Groll had a three-year, $105,000 loan, enough to buy Shag Salon and renew its 1,850-square-foot commercial lease for a decade. Even better was her interest rate: 9 percent. The brainchild of Harvard MBAs Will Davis and Evan Baehr, Able offers business owners one- to three-year loans of $25,000 to $250,000 at 8 to 16 percent interest -- but with a twist: Borrowers must raise the first 25 percent from friends and family. What were really doing is trying to find the people who are being missed by traditional banks and even nontraditional online lenders, Davis says. Able conducted a beta test prior to its official launch in 2014, tweaking the terms and procedures with each loan.
For the original version including any supplementary images or video, visit https://www.yahoo.com/news/2-services-help-friends-family-200000246.html
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"Know GMO" is a documentary in development meant to provide "an uplifting discussion about food" to help silence the noise and open the ears to facts that Scientists across the world are speaking. Robert Saik is the executive director of the movie project and his son Nick Saik is filming it.
The Genetic Literacy Project reported on Saik's trip to Africa to shoot part of the documentary.
According to the GLP, Saik interacted with and interviewed banana and cassava farmers, scientists and members of Parliament — those looking for a solution to their common problems, disease threatening staple plants in Africa. The available transgenic solution is being fought against by anti-GMO activists despite the benefits the technology provides.
..To Saik, genetic engineering is a continuation of what agricultural scientists have been doing, and he believes it is key in addressing global food challenges like malnutrition and hunger. The well thought title of his address mirrors well the controversies around GMOs. Any view or fact brought forward in support of GMO should not be taken on faith alone; whether it is "Know GMO" or "No GMO", one has to lend ears to scientific facts to be able to make informed decisions.
When I went to one of the scientists working on developing the transgenic banana and told him there was a crew coming from Canada to film, a movie "Know GMO," and the director was interested in interviewing him, he was skeptical. "What do those people want?" he said. "I don't have time for them".
I explained to him that the title was "Know GMO" not "No GMO" and the crew only wanted to ask him questions about the work he was doing, including the challenges he was addressing by using genetic engineering as a tool in his crop improvement tool box. The crew leader had told me the various interviews would enable them to bring out the science, the facts, the agriculture and food challenges being addressed as they are despite activists' fear mongering campaigns claiming GE foods would cause sterilization of women and cancer. The movie would focus on scientists who are concentrating on helping farmers facing real problems and inform the public about the emotionally charged scare tactics used by its opponents.
Nick Saik's view of the "Know GMO" forthcoming Documentary:
"The intention of the 'Know GMO' project is to tell the story of modern agriculture from a fresh perspective."
He was quick to say he is anti-nothing; rather he is pro food, pro organic, pro GMO, and pro regulations when necessary. He attributes his passion to the need to ensure people stop being afraid of their food. Nick thinks most documentaries on food have been focusing on propagating fear because fear sells. Know GMO will focus on facts and perspectives from scientists, policymakers, consumers and the evidence from farms, from Hawaii to Uganda and Kenya in Africa…
…Nothing could explain Nick's passion for this cause than the fact that Nick, a recent newlywed, skipped his honeymoon and came with his new wife Toni to Africa to film how modern biotechnology is helping to solve farming problems in this part of the world. The crew, including Scott Culbertson and Bill Paris from Hawaii, boast over 20 years of film experience.
What are challenges facing the growing of staple foods in agriculture for which GM technology offers a potential solution? Banana bacterial wilt is infecting Uganda's banana farms, savaging their income and depriving the population of a main source of carbohydrates; and cassava brown streak disease is destroying another key carbohydrate source. Banana and cassava genetically engineered to be resistant to these diseases are locked in Uganda's research facilities because of the actions of a small but loud minority armed with the ideology of fear more. They are frustrating the voices of reason whose strength and hope lie in the scientific facts, proven over the years, that GM foods are safe and the vigorous safety tests they go through even make them safer.
GLP continued its piece by noting that when visitors come to Uganda, one popular place to visit is the source of River Nile, the world's longest river, which is a 2-hour drive from Kampala. The Know GMO crew did just that. Uganda does not use the Nile water for irrigation but it helps water crop fields in Egypt. Nick in his experience of the Nile few years back in Sudan, was met with gun wielding thugs who ordered him to put his shaking hands on his head and told to look down. Nick intimated the last thing he thought he would see before he was killed were his toes. He began to equate the Nile with trouble.
In Uganda, where the Nile begins its northerly journey, Nick and the crew rode in a boat; everything was peaceful, with birds of varied plumage pecking occasionally. There were no problems. It's clear there is no problem with the river, it's with the people around the Nile. That lesson applies to the swirling debate around the technology of genetic engineering, recombinant DNA technology. You could call it tweaking, playing God, or whatever, but there are no serious scientific dangers presented by this technology as it's presently used–and it's available to address a problem that is threatening livelihoods of banana farmers in Mpigi that Saik went to visit.
The same technology could help rescue cassava crop from the scourge of brown streak disease which causes up to 100% yield loss in non transgenic farmers preferred varieties. Genetic engineering is a tool being used to introduce resistance to the farmers varieties while preserving the taste of cassava and other agronomic attributes that farmers are interested in–yet it's being blocked.
Whereas the Nile may not make irrigation sense in Uganda because of its other sources of fresh water and enough rainfall, to the Egyptians, the Nile water means everything. Modern biotechnology in the same way; it may not make sense to those who are spoilt for choices, but for African farmers whose staples and livelihoods are being threatened because conventional and organic methods are failing to address their problems, biotechnology becomes a necessity and not just a debate topic for the privileged class in the west.
Filed under: Farmer Gene, Food And Agriculture, Africa, Agri-Trend, Agricutlure, BIOTECH, GE food, Genetic Literacy Project, GMOs, Know GMO, Nick Saik, Rob Saik, Uganda


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About Maiora 20
With 3 comfortable cabins (a master double bed, VIP double bed and a twin bed cabin with the possibility of an additional bed) and 3 toilets, this luxury yacht can provide accommodation for a maximum of 6 – 7 guests.
Sporty but elegant, Maiora 20 meets the demand for the highest levels of comfort offering numerous features not usually found on a motor yacht of this size.
Motor yacht - Maiora 20 - charter price list 2018.
All prices are in € per yacht per week. Yacht crew service, tender, water sport equipment and VAT included. Fuel, mooring or anchor fees, provisioning, personal laundry, communications and banking expenses not included.
Obligatory extras
- APA (Advance Provisioning Allowance): 30% of the charter price (For provisioning, fuel, mooring or anchor fees and other extra expenses which will all be specified in an addendum attached to the charter contract. At the end of the charter period the charterer will be presented with invoices for all the extra expenses and any unused funds will be refunded in full. If the funds prove not to be sufficient the captain will advise the charterer accordingly and additional expenses will be settled upon disembarkation.)
- sojourn tax / person / day: approx. 1,07 € (in accordance with laws of the Republic of Croatia)
- safety deposit (if required): payable by credit card (Visa or MasterCard)
For information on other available services and equipment, please feel free to contact us.
Embarkation / disembarkation / in Split
- embarkation time: Saturday from 17:00 h (if not differently agreed)
- disembarkation time: Saturday till 09:00 h (if not differently agreed)
For Your comfort
Generator, air condition, water maker, deck / fly sunbathing cushions, cockpit table, El. refrigerator, microwave, ice maker, TV / DVD, audio component (Hi-Fi), Internet Wi-Fi, swimming ladder, gallery equipment, bedclothes and towels.
Just for fun
Jet Ski (optional), waterskiis, wakeboard, snorkeling equipment, donut.
Subject to Yacht charter company and MYBA terms and conditions.
Technical data
|20.80 m
|5.60 m
|1.60 m
|MTU Diesel 2 x 1250 HP
|Cruising speed:
|22 knots
|Fuel consumption:
|250 l / h


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Grass is greener online dating
This is what you have been waiting for right? I am finally going to be tackling the grass is greener syndrome. I want this guide to be extremely insightful so I am going to be covering a wide variety of topics, I am going to teach you what the GIGS grass is greener syndrome is.
I will give you real life examples of men who have gone through this. I will explain why sometimes the GIGS can work. I will also dive in to how the GIGS can be a very dangerous thing.
Oh, and how could I forget that I am going to be applying all of this to your ex boyfriend. Lets start by defining the GIGS. If you would like to learn how to get an ex boyfriend back who has GIGS please click this link.
I have a confession to make… The first time I heard of the grass is greener syndrome was when a reader of this site pointed it out to me.
That is definitely not the case anymore as I did extensive research to make sure I knew everything there was to know. The Grass Is Greener Syndrome- The belief that what you currently have in your life is no longer good enough or adequate. As a result, you begin to believe that there are better things out there. The GIGS is mostly used to refer to people in relationships but it can also be applied to people who are in jobs. Of course, since we are trying to gain insight into your ex boyfriend here we are going to be using the grass is greener syndrome in the context of relationships.
If I had to pick out one of the most frightening aspects of this entire thing it is that you could be doing everything perfectly in your relationship, you could literally be the best girlfriend in the world to your boyfriend and he could still have a bout of GIGS.
Lets take a look at how this phenomenon can apply to your ex boyfriend. Do you have a chance of getting him back? So, lets take a moment and look at what your ex boyfriend may be experiencing assuming he got a bout of the GIGS.
In my opinion, there are really three outcomes that can occur when it comes to your ex boyfriend. The way this works is actually pretty simple. You and your boyfriend are doing great. You love him and he loves you. FYI there is no question in his mind about that.
Actually… now that I think about it I have always thought that from the moment I started dating her. Lets pretend that you and I are dating. Throughout our relationship you are horrible to me. You are controlling, manipulative and get angry at every little thing I do. I think I can do better. It probably is which is why I left. Nothing ever fulfills him and as a result he opts to look elsewhere for it. In other words, he jumps from relationship to relationship trying to find someone who is going to fulfill him.
Unbeknownst to him just the way he is, always thinking he can do better, prevents him from ever sustaining a long term relationship with anyone. How can you tell if your ex boyfriend is like this?
Admittedly, this particular mindset on a man tends to be really rare. However, there are warning signs that you can keep a look out for.
Learn about how you can get an ex boyfriend back by clicking here. Obviously there are two trains of thought when it comes to GIGS. One portion of people believe that ending a relationship because you think you can do better usually works out.
I like to call this phenomenon, the setting the bar effect. If your ex boyfriend could rate his experience having a relationship with you on a scale what do you think he would give you? Lets use the example I gave above in the last section about you being a horrible girlfriend and doing nothing but berating him throughout the entire relationship as a placeholder.
Given this particular situation what do you think your ex boyfriend would rate you as? Where would he set your bar? How about a 2. Alright, in this particular instance if your ex boyfriend can find a girl that can put provide him with a situation better than a 2. Heck, maybe you were the best girlfriend that he ever had in his entire life and he gives you an 8 out of Well, if your ex ends up getting the GIGS he may potentially leave you because he thinks he can do better.
Anyways, lets say that he does find a girl that he ends up dating for a little while but he only sets her bar at a 7. This is a case where the grass is not greener and he is going to be really kicking himself for letting you go.
In other words, he will be filled with an extreme amount of regret. He rated your overall relationship as a 6 out of Well, when he finally does get in a relationship with someone else he may found out that she is a 6 out of 10 as well.
For him in this scenario the grass is not greener. Remember, the grass is only greener if he can find someone who can beat the bar that you set. Of course, the other fly in the ointment for him is the fact that the grass may be a little greener at first but it almost always never stays the same.
Everyone here is aware of the honeymoon period in a relationship right? Well, for those of you new to the game the honeymoon period is this period of the time at the beginning of a relationship where you have this constant emotional high and your significant other can do no wrong. This is normal for every single romantic relationship that has ever been formed since the beginning of time. Anyways, after I leave the relationship I determine that my experience with you was a 7 out of Then the inevitable happens, I meet a new girl and start a new relationship with her.
At the beginning this new girl and I are going to be going through a honeymoon period so my experience in that relationship is going to be super high probably a 9 or a Wow, a 9 or The grass was greener right? The honeymoon period begins to wear off and that is where the true test of a relationship begins.
Pretty soon this new girl and I start fighting a lot. That rating suddenly drops to a Then I begin to suspect that she is cheating on me. Now the rating is down to Let me give you an example. When you are driving around in the car and listening to the radio what do you do when you hear an amazing song for the first time?
I immediately memorize a few lyrics to the song and send a text message to myself so I can look it up later and buy it. Once I have bought the song I listen to it about 50 times over and over again. This is a bit of an issue because after you listen to a song that much it kind of loses its appeal after a while. The same principle applies here. Sure, some honeymoon periods last longer than others but the end result always seems to be the same, it ends. It just means that your ex boyfriends initial view of the relationship he is currently in may go down a little bit.
For example, that 9 he experienced during the honeymoon period may turn into a 7 or an 8. I actually have two real life examples that I can tell you of how the grass is greener syndrome works. I am going to give you an example of a woman who I communicated with on this site who ended up getting her ex boyfriend back after he got GIGS. In other words, I am going to give you an example of a case where the grass was not greener on the other site. In addition to that I am going to be giving you an example of a case where the grass WAS greener on the other side by talking about the mindset of someone I know who met his future wife after a case of the GIGS.
I suppose we can start with what you really want to hear. His name was Brad. Something was different about her though. Brad was noticing her in a different light. They spent the entire night together swapping stories, philosophies and dreams. By the time the party was over at 2am the two were still talking. From that moment on they were inseparable. As I am sure you have already predicated Brad and the girl ended up dating. Their relationship was actually quite good.
Sure, there were fights here and there but generally speaking everything was great most of the time. One day Brad had a strange epiphany. Actually, it turned out to be the day before his 30th birthday. You see, his parents kept urging him to marry the girl and as time went on he kept finding excuses not to man up and propose. So, he decided to break up with her so he could start his search for his future wife. Little did he know that the girl who asked me to remain anonymous was waiting to be his future wife all along.
She told me her story and I point out that she was a very good girlfriend to this guy and eventually he is going to realize his mistake. Again, she comes back to me begging for me to tell her what to do.
The Grass is Greener is the leading lawn care and tree shrub company serving clients throughout Chattanooga, Cleveland TN and in the Dalton, Ringgold GA areas. We are licensed in Tennessee and Georgia and are committed to providing you with the highest quality lawn possible. Is the Dating Grass Greener on the Other Side? Online Dating Boundaries and Giving Out Your Telephone Number Online Dating and They Want More Pics? Don't! Signs That He Likes You - From Guys' Point of View 5 Ways to Know If Someone You Met Online is a Fraud: Guest Post Why You Should Let Them Walk Away and Peace Out.
Total 3 comments.
#1 17.09.2018 РІ 20:54 Eslamhero:
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#2 22.09.2018 РІ 10:03 Duki818:
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#3 22.09.2018 РІ 22:09 Osman-Abdella:
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Emma Slater Biography
23 Oct Michael Waltrip, the Nascar driver, is rumored to be dating Dancing with the Stars partner Emma Slater. However, a report from Gossip Cop says that the rumor is bogus. A source said that Waltrip, 51, and Slater, 25, "have fallen for each other since being paired up for the show in September," according to. 9 Oct Emma Slater passionately kisses Sasha Farber days after accepting his romantic proposal on Dancing With The Stars. After becoming engaged on national television just days ago, Emma Slater and her new fiancé Sasha Farber couldn't have looked more in love as they spent Sunday. 27 Oct Of all the Season 19 Dancing With The Stars couples, the last one we expected to start hooking up was Emma Slater and Nascar driver Michael Waltrip.
She is best known for her appearances on Dancing with the Stars. Emma Slater is from Tamworth in England, where at an early age, she joined local stage schools taking part in numerous productions.
In the early years of competing, she won a number competitions in England. The two are now engaged. Slater has numerous titles to her name, notably in aged 16, she won the British Under 21 Latin American Championships at the Blackpool Closed In Slater joined the stage show Burn the Floor for the Check this out tour initially but later joined the cast for an extended run at the Longacre Theatre on Broadway New York City and a world tour covering: In MarchSlater joined the fourteenth season on the American edition of Dancing with the Stars as a troupe dancer, where she danced and choreographed all of the opening dance numbers including the finale.
Later that year, she choreographed Usher at the Billboard Music Awards in Las Vegas including partner work with co-choreographer and also troupe member, Sasha Farber.
Emma & Sasha proposal - Cirque du Soleil Recap/Results - Dancing with the Stars
After three seasons of being a troupe member, she was http://24dating.me/fek/is-carbon-dating-100-percent-accurate.php as one of the new female professional dancers for the seventeenth season of the show in September Her celebrity partner was award-winning comedian, singer and actor, Bill Engvall.
For season 18she returned as a professional dancer partnered with movie actor Billy Dee Williams. Due to a back injury on Billy Dee, they withdrew from the competition on Week 3.
For season 19she was paired with race-car driver Michael Waltrip and they finished in 7th place. Slater did not participate in Season 22 of the reality show.
Slater returned for season 23 and was paired with former presidential candidate Rick Perry. They were eliminated on week 3 of competition and finished in 12th place.
Dancing with the Stars: Michael Waltrip, Emma Slater Rumored to be Dating; But Likely Not
They made the finals of the competition but were eliminated on night one, finishing in 4th place. Slater performed with Tommy Chong.
Slater performed with Alek Skarlatos. This season was Slater's first ever win in the competition. This also makes her the first British person both celebrity or professional dance partner to win the show.
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Retrieved 28 September Retrieved 23 July Retrieved 18 September Did the Right Couples Go Home? Retrieved 4 October Under 21 Latin Saturday, 27 May ". Theatre World, Volume Archived from the original on 27 September Retrieved 2 September Retrieved 18 July Retrieved 7 October Retrieved 22 September Retrieved from " https: Views Read Edit View history.
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This page was last edited on 14 Februaryat By using this site, you agree to the Terms of Use and Privacy Policy. Sasha Farber —14; — [1].


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