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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The beta-sheet is one of the two common structural motifs in proteins.n Understanding its folding mechanism is therefore important to understand to what extend large proteins fold. The objective of this application is to develop new knowledge at the single residue level of beta-sheet folding pathway using beta-hairpin as model. The hairpins proposed in this work are chosen to probe the factors that contribute to its stability like the intrinsic amino acid turn tendency, the stability of the hydrophobic cluster, the interstrand hydrogen bonding, and interstrand side-chain-side-chain interactions. Thus, our long term goal is to understand the physical and chemical factors that regulate the beta-sheet formation. The rationale for the proposed research is that knowledge of folding mechanism at the single residue level will provide better information to performed site-directed mutagenesis, help in the synthesis of new peptides and proteins with specific functions, and will allow taking control of the folding/unfolding process. The proposed work is innovative because higher structural information at the single residue level of the folding/unfolding of beta-hairpins will be available. With respect to expected outcomes, the combination of the work proposed in specific aims is collectively expected to identify and quantify the factors that are responsible for beta hairpin folding/unfolding mechanism. During recent years, great improvements in the time and spectral resolution of protein folding experiments have been achieved;resulting in new physical and chemical knowledge about the correlation of the unfolded and folded states. Also, an increase in theoretical calculations with increased computer capacity brings the protein folding dilemma to a new level of complexity. However, there are still many fundamental questions on the nature of the folding pathways. Basically, these questions are related to where and how the folding initiation step takes place and on what range of timescale does it occur. Furthermore, it is important to establish how the primary amino acid sequence encodes the folding pathway. This knowledge serves to predict the folding mechanism for a particular protein from its sequence and structure. Moreover, since the genome era is finally mature, new de novo designed peptides and proteins with certain unique functions such as therapeutic agents and drug delivery agents could be developed. The beta-sheet is one of the two common structural motifs in proteins. Understanding its folding mechanism is therefore important to understand to what extend large proteins fold. Thus, our long term goal is to understand the physical and chemical factors that regulate the beta-sheet formation. The objective of this application is to develop new knowledge at the single residue level of p-sheet folding pathway using p-hairpin as model. Beta-hairpin is the p-sheet basic building block consisting in two parallel strands joined by a twisted turn. Our working hypothesis is that higher structural detailed information of the beta hairpin folding pathway, important for the understanding of the extend of protein folding, can be obtained by site directed amide carbonyl isotope labeling with IR spectroscopy and laser induced temperature jump using IR detection. The first type of experiments will provide information on the thermal stability and the second on the formation of the folding-unfolding dynamics. A systematic study of a series of p-hairpin analogues with the same strands and different turns or a common turn with strand variation are proposed to study the local conformational change along the primary amino acid sequence. The rationale for the proposed research is that knowledge of folding mechanism at the single residue level will provide better information to performed site-directed mutagenesis, help in the synthesis of new peptides and proteins with specific functions, and will allow taking control of the folding/unfolding process. Therefore, the specific aims of this work are to identify and quantify the: (1) effect of moving the hydrophobic cluster position across the strand relative to the turn to the thermal stability and folding kinetics; (2) the contribution and position dependence of side-chain-side chain interactions to the conformational stability and dynamic properties for the folding/unfolding mechanism; (3) the contribution and position dependence of cross-strand electrostatic and aromatic side-chain interactions to the folding/unfolding mechanism; (4) the contribution of the turn sequence to the overall stability and dynamics.
|Cantres-Rosario, Yisel M; Acevedo-Mariani, Frances M; Pérez-Laspiur, Juliana et al. (2017) Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment. PLoS One 12:e0181779
|Mariño, Yobana A; Verle Rodrigues, José C; Bayman, Paul (2017) Wolbachia Affects Reproduction and Population Dynamics of the Coffee Berry Borer (Hypothenemus hampei): Implications for Biological Control. Insects 8:
|Martínez-Rivera, Freddyson J; Pérez-Laspiur, Juliana; Santiago-Gascot, María E et al. (2017) Differential protein expression profile in the hypothalamic GT1-7 cell line after exposure to anabolic androgenic steroids. PLoS One 12:e0180409
|Zenón, Frances; Jorge, Inmaculada; Cruz, Ailed et al. (2016) 18O proteomics reveal increased human apolipoprotein CIII in Hispanic HIV-1+ women with HAART that use cocaine. Proteomics Clin Appl 10:144-55
|Rivera-Rivera, Yainyrette; Vázquez-Santiago, Fabián J; Albino, Elinette et al. (2016) Impact of Depression and Inflammation on the Progression of HIV Disease. J Clin Cell Immunol 7:
|Martínez-Rivera, Freddyson J; Rodriguez-Romaguera, Jose; Lloret-Torres, Mario E et al. (2016) Bidirectional Modulation of Extinction of Drug Seeking by Deep Brain Stimulation of the Ventral Striatum. Biol Psychiatry 80:682-690
|Suárez-Arroyo, Ivette J; Rios-Fuller, Tiffany J; Feliz-Mosquea, Yismeilin R et al. (2016) Ganoderma lucidum Combined with the EGFR Tyrosine Kinase Inhibitor, Erlotinib Synergize to Reduce Inflammatory Breast Cancer Progression. J Cancer 7:500-11
|Suárez-Arroyo, Ivette J; Feliz-Mosquea, Yismeilin R; Pérez-Laspiur, Juliana et al. (2016) The proteome signature of the inflammatory breast cancer plasma membrane identifies novel molecular markers of disease. Am J Cancer Res 6:1720-40
|Wang, Hai-Yang; Huang, Kun; De Jesús, Melvin et al. (2016) Synthesis of enantiopure 1,2-azido and 1,2-amino alcohols via regio- and stereoselective ring-opening of enantiopure epoxides by sodium azide in hot water. Tetrahedron Asymmetry 27:91-100
|Colon, Krystal; Perez-Laspiur, Juliana; Quiles, Raymond et al. (2016) Macrophage secretome from women with HIV-associated neurocognitive disorders. Proteomics Clin Appl 10:136-43
Showing the most recent 10 out of 120 publications


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What is a Animated Whiteboard Video
An Whiteboard Explainer Video is a short, fun and informative video that is usually two - 3 minutes long but in some cases less. An Animated Whiteboard Video is a short, enjoyable. It captures and retains viewers attention more efficiently than just text or boring videos.
Whiteboard Video videos with a specific goal: understanding. They use clear and concise language along with entertaining and useful visuals. The video format can cater to as a near-perfect package for presenting ideas, products, and services in a form that not only explains but attracts attention.
Feel intimidated with the notion of creating an explainer video? There's no need to be-- they just represent another excellent way to get your content out to your target audience. Besides the really big brands that we are all familiar with, a lot of lesser-known companies and even small start-ups are using them.
Explainer videos 'explain' what you do swiftly and simply so anyone can comprehend it. It's kind of like CliffsNotes for your website-- a concise and short version of exactly what your company does.
Online Video Marketing
Web Video Marketing is emerging as an effective platform to provide an impactful interactive and highly targeted message. One type of content that is still underutilized is online video. We have different kinds of videos to satisfy each business's requirements If you need to show people around your site we suggest a Custom Video Presentation. Internet Video Marketing is emerging as an effective platform to provide an impact because they deliver an interactive and highly targeted message.
Web Video Marketing
These days Website Video Marketing need to be a significant part of broadening your website. A video hosting website can increase audiences for your video, however a boring video will defeat the function. When you develop a video for an website you require to offer out beneficial details in a method that keeps your viewers' attention. Choose on a consistent tone for your marketing videos. You need to now have a much better understanding of what it takes to develop an effective Web Video Marketing method utilizing Explainer Videos.
Do you want to enhance your Video Marketing?
With a professional Animated Whiteboard Video, you can rapidly put your logo into movement and highlight your brand name in your next video. Whatever style fits your brand name, you need your video to get your viewer's attention right away and leave them with a favorable impression. And you only have a couple of seconds in your video to get your brand name identity, so you have to make them count.
Every day countless new videos are created so it is becoming a growing number of challenging for a video to stand apart. Numerous viewers stop enjoying after the first 15-20 seconds due to the fact that the video is not engaging. With a Animated Sketch Video you can increase your follow through rate and reveal your viewers that you are serious about your site. A Animated Sketch Video, sometimes referred to as a Whiteboard Animation, Whiteboard Video, or Explainer Video, can really assist your brand stand apart.
Add Video to Your Website
Adding videos to your website is an excellent way to enhance the value of your websites, while likewise taking advantage of the possible additional traffic from video search engines. There's much more involved than periodically posting an online video on your site. You require a Video Strategy. If your website's function is to raise awareness about a specific cause, adding educational videos could be effective. The home page may be the ideal spot for your cause awareness video whereas private item pages may be much better suited for the how-to videos. Just as it's crucial to regularly include posts and blog posts to your sites, frequently adding online videos is likewise important.

subset: commoncrawl


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How Much You Need To Expect You'll Pay For A Good brake repair memphis tn
With an entire brake analysis, we are able to determine exactly what your brakes require for superior stopping electricity.
never ever knowledge any put on. In actual apply though, They could celebration some wear due to malfunction on the AutoPark parking brake attribute (brake gets applied while you're likely down the road), OR – – Over a chassis that features a handbook signifies of implementing the parking brake (like a foot pedal or lever), someone has mistakenly pushed the motor vehicle while the guide brake was applied.
The main elements of a braking procedure, detailed below, will must be repaired or changed as component of standard brake service and upkeep in the course of the conventional lifespan of a auto. Get to find out the indicators today to aid avoid a headache tomorrow.
Employed mainly in rear braking devices, brake shoes push from the inside of your respective brake drum to trigger friction, slowing, or halting your car or truck.
Should you hear any brake sound such as screeching, squeaking or grinding, you should quickly have your brakes checked.
These people charge much less than RV dealerships do, and the standard of their get the job done is just as very good, if not greater.
Motor households, journey trailers and campers are fantastic to individual, but before you purchase 1, you should ensure you understand how much it can cost to keep it repaired.
The brake system is one of A very powerful components of your automobile, in addition to a thoroughly Doing the job technique will drastically help your ability to provide the vehicle to a cease promptly.
Planning to Obtain your Kia vehicle's oil improved in Brandon, Tampa, Wesley Chapel or Tampa Bay? There are many places it is possible to prefer to go in the region - no matter if it's a small unbiased shop, A different vendor, or a large chain.
Through the estimate, only you decide the get the job done for us to accomplish and select the brake service approach that is right on your car or truck and its overall performance.
2. It is remotely probable that the large fusible link (60 amps or so) that protects the relay and motor, may very well be blown. We've under no circumstances observed this happen as however, nevertheless it needs to be viewed as. A single could examination for this rather very easily, by pulling the connector off with the relay, and examining for your existence of twelve volts on among the two substantial wires in the feminine connector.
Courtesy Kia of Brandon's staff members understands Kia autos within and out - they have been exclusively experienced to operate on Kia cars through several hours of considerable coaching. Folks across Tampa Bay understand that whenever they require trusted Kia service, they're able to flip to Courtesy Kia of Brandon for oil modifications or almost every other service They might be in the market for.
It is important to know you could recover pieces and get monetary savings on brake pad repair by acquiring the pieces and providing them on the mechanic.
So – – Assuming the cables on their own are in very good issue, this leaves our remaining variable to get the prolonged hex nut about the actuator shaft – – depicted as #1 within web the illustration over. It is essential to notice that this actuator is shown in the BRAKE ON problem – – Test TO VISUALIZE IT With all the SPRING COMPRESSED within the BRAKE OFF ailment. Along with the brake OFF along with the actuator shaft extended to its mechanical limit, we'd want to regulate the very long hex nut just to the point wherever there was no slack within the cable method – – no more, no a lot less. If we then take away the hydraulic pressure with the actuator, the spring will broaden, and the piston and shaft will shift to the best – – implementing the brake. Consequently the brake lever within the drum will transfer about an inch, the cable going to the lever relay will go about the same inch, the cable heading FROM the relay into the actuator will move about one.


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A Q&A With Mike Keenan, Managing Director, Retail Loss Prevention, TAL Global
The only way to reduce shrink and keep people safe is to attain advocacy from the top down.
LP has evolved over the years to become a critical component of the entire organization. Here, Keenan provides reasons why senior leadership teams must work closely with LP to lower shrink, increase profitability, and keep people safe.


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Home › Forums › Astrology Forum for Free Horoscope Reading and Predictions › Marriage Success › Reply To: Marriage Success
His chart has the problem, after may 2017 his vimsottari das changed right he is in Venus-Mercury dasa and they are 7th and 8th lords and posited in 8th house and mars is aspecting them
2018 march you anthar dasa will change which will bring good to you. Just wait and take any serious decision after your birthday
Pray for lord Shiva on Mondays go to temple if possible donate white rice on that day.
donate red gram-1 kg on Tuesdays for 5 weeks


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Julie Norby, 64, Green Bay, passed away following a very long, and for the most part successful fight, with Carcinoid, a rare, slow moving, and unusual cancer on Monday, October 1, 2018 in her home at Ridge Pointe CBRF in Green Bay, part of Compass Development where she resided since 2009. She was born April 25, 1954, in Beloit, WI to Helge "Ollie" Norby and Audrey B. Norby. They were dedicated parents to both their girls and were especially active advocating for Julie's special needs.
Julie grew up on a farm in Beloit Township and there she especially enjoyed the animals and riding her bike. She attended Blackhawk School for the Handicapped and then Glen Oaks School in Janesville, WI graduating from there in 1974. Julie attended Kandu Industries for several years until her move to Green Bay. She was active in Special Olympics in Beloit and also the Nazarene Church in Rockton, IL., activities that both she and her mother enjoyed sharing together. She graduated with a competency certificate in 1996 as a Food Service Aide from Blackhawk Technical School in Janesville, WI. She loved to help out in the kitchen and took it upon herself to always clean up the dirty plates after a meal, a job she was zealous about! Over the years jigsaw puzzles and small stuffed animals as well as a miniature toy school bus were always in use and never far away. She traveled with her mother throughout the United States and Canada visiting friends and relatives. Julie's life was rich and varied. This was primarily due to the tireless efforts of her mother to promote any and all activities that would enrich Julie's life.
Julie and her mother Audrey moved to Green Bay from Beloit, WI in 1997. Since moving to Green Bay she had participated in the day services at the ASPIRO Dousman location and also with the Packerland Jets Special Olympics athletes. Julie attended the Senior Program at ASPIRO and was in Special Olympics, most recently bowling and playing bocce, and previously basketball skills and track too. She was very social and enjoyed the many friends she has made in these organizations over the years. These were activities and programs that Julie participated in with the support of her mother and later with her sister, Linda. Ramp bowling with her sister was a "team effort" they both enjoyed enormously.
In 2005 Julie was paired up with Laura Klarkowski through ASPIRO's Advocacy Program (later renamed "Friends Forever Program" but now, unfortunately, defunct). Laura was Julie's very special friend and her impact on Julie's life brought much joy to Julie and her family.
Julie is survived by her sister and brother-in-law, Linda and John Stein, her niece, Emilie Stein; nephew, John Oliver "Ollie" Stein (Tricia); great-niece, Willow Grace all of Green Bay, and her aunt, Stella Birkett in Kearney, AZ; and many cousins.
Julie was preceded in death by her father in 1975, and recently her mother in August. Her grandparents, aunts, uncles, and some cousins had passed prior to Julie. Julie loved her family and they all, to varying degrees, added richness to her life with their love and support.
In lieu of flowers, donations may be made in Julie's name to ASPIRO, 1673 Dousman St., Green Bay, WI 54303, a community based organization that helps people with disabilities live their best lives. A special thank you is extended to Aspiro - Dousman Site, Special Olympics Packerland Jets, Ridge Pointe/Compass Development, and Unity Hospice, as well as Dr. Bozena Biernat at Bellin Family Clinic in DePere , Dr. Hassan Tahlsidar at Bellin Cancer Team, and Dr. Peter Fergus with Prevea, and Lakeland Care, Inc. for their loving care and emotional support over the years. "It takes a village" to see to it that individuals with special needs can reach their full potential and Julie was fortunate to have one.
Family and friends attended services at Blaney Funeral Home, 1521 Shawano Ave., Green Bay, WI with visitation from 4:30 to 6:30 p.m. on Thursday, October 4th, followed by the Funeral Service at 6:30 p.m. with Chaplain Steve Fewell officiating. Burial will take place at Eastlawn Cemetery in Beloit on Saturday, October 6 at 1:30p.m. with a short grave side service. Blaney Funeral Home, Green Bay, WI is assisting the family. To send online condolences, please go to www.BlaneyFuneralHome.com.


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Swift cc
CC Bootloader. CC Bootloader is a. --code-loc 0x1400. (Small Device C Compiler). Then it should be as simple as issuing. make. from the root directory of the.
EXAMPLE LETTER OF CREDIT - HomepageBMO Credit Card Holders: To register for Online Banking, you will need your BMO Credit Card. You will also be asked some additional security questions.The Crimson Swift is a red bird that can be caught using the Hunter. Old School RuneScape Wiki is a FANDOM Games Community. Content is available under CC-BY-SA.Swift Code is a standard format of Bank Identifier Codes (BIC) and it is unique identification code for a particular bank.Mainstreet can help you reach your goals. Whether it's a dream vacation, a new car or home, or college tuition. Join Mainstreet Credit Union for your banking needs.
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Taylor Swift's attorney: Radio host groped her, then - CNN
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The Crimson swift is a red bird that can be caught using the Hunter skill, granting the player.Webinar: Local Government Ethics. Learn about the latest lawsuits and legal cases related to ethics in local governments as part of an hour-long. Foster Swift.
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Banks also used the codes for exchanging other messages between them.Passat cc manual - pantech breakout. sn667wb user guideand also pantech swift manualand also panasonic rr qr80 manual bookand also pc game.Credit union offering free rewards checking, mobile banking, loans, and mortgages in Maine.Search the world's information, including webpages, images, videos and more. Google has many special features to help you find exactly what you're looking for.
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Techniques for getting started with C libraries from Swift, using Apple's CommonCrypto library as an example.


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Laird Barron is the author of the novel The Croning and three collections of short fiction: The Imago Sequence, Occultation, and The Beautiful Thing That Awaits Us All. His work has appeared in many magazines and anthologies. An expatriate Alaskan, Barron currently resides in the wilds of upstate New York. Michael Shea is an American fantasy, horror, and science fiction author. He is the winner of two World Fantasy Awards, and has been nominated for Nebula and Hugo awards. Shea lives in Healdsburg, California.
"Another brilliant engagement with weird fiction from a writer fast becoming a modern master." --Jeff VanderMeer, author of Annihilation and Finch "Relentlessly readable, highly atmospheric, sharply and often arrestingly written--Barron's prose style resembles, by turns, a high-flown Jim Thompson mixed with a pulp Barry Hannah." --Slate "Laird Barron has, in a remarkably short period of time, emerged as one of the leading writers of contemporary weird fiction." --S. T. Joshi "With sharp prose and wise, original stories, Barron has repeatedly proven himself one of the strongest voices in the field. This collection is a must read." --Sarah Langan, author of Audrey's Door and The Keeper "Laird Barron is one of those writers who makes other writers want to break their pencils. I'm serious. His work is that good. Worse than that, he's an original (damn him!), and the finest writer to join the ranks of the dark fantastic in a long, long time." --Norman Partridge, author of Dark Harvest and Lesser Demons "For my money, Laird Barron is far and away the best of the new generation of horror writers." --Michael Shea, World Fantasy Award-winning author of Polyphemus "...one senses that he has the potential to change the expectations of the next generation of readers by elevating the genre to a new standard of excellence." --Lucius Shepard, author of Trujillo "If you think there aren't any new Richard Mathesons or Harlan Ellisons out there, you need to read Laird Barron." --Stewart O'Nan, bestselling author of A Prayer for the Dying "Heartbreaking, hilarious, sophisticated, and gory, these stories will thrill, trouble, and haunt Barron's fans and have newcomers scrambling to search for his other work." --Publishers Weekly, starred review


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Take your time for a moment, see some collection of sitting room decoration. Now, we want to try to share these some portrait to find best ideas, we really hope that you can take some inspiration from these awesome portrait. We hope you can inspired by them. Perhaps the following data that we have add as well you need. Decorative mirror above fireplace beautiful living room decor blue sofa popular wall color schemes plus sloped ceiling ideas, Inspiring decorative mirror above fireplace beautiful living room decor blue sofa popular wall color schemes plus sloped ceiling ideas part function dining other rooms editorial.
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Lawrence R. Rabiner, Biing-Hwang Juang
Mentioned 3
Provides a theoretically sound, technically accurate, and complete description of the basic knowledge and ideas that constitute a modern system for speech recognition by machine. Covers production, perception, and acoustic-phonetic characterization of the speech signal; signal processing and analysis methods for speech recognition; pattern comparison techniques; speech recognition system design and implementation; theory and implementation of hidden Markov models; speech recognition based on connected word models; large vocabulary continuous speech recognition; and task- oriented application of automatic speech recognition. For practicing engineers, scientists, linguists, and programmers interested in speech recognition.
As described in several books, the process of recognition of isolated words consists of the following:
Because speech signals have different duration, their lengths are aligned by Dynamic Time Warping (DTW) technique, so that N is same for all templates. It can be done during training.
My question is: How to change length of test signal? I can not use DTW on it, since I do not know to which class it belongs. Should I use "time stretching" algorithms, preserving pitch and if I should, how this will affect recognition accuracy?
You do not need to change the length to make a match. You extract features from reference samples and test samples, they all have different number of frames. Then you apply DTW between each reference and test thus aligning them. As a result of DTW runs you get the score of match between test sample and each of the references. What you do is you stretch non-uniformly each reference sample to match with test sample. Because you compared each reference with single test, you can use DTW scores in comparison. So you select the reference with best score as a result.
For details and ideas of DTW speech recognition check this presentation.
If you want to get closer to ideas of speech recognition with DTW, you can read a book Fundamentals of Speech Recognition 1st Edition by Lawrence Rabiner, Biing-Hwang Juang.
I am implementing a software for speech recognition using Mel Frequency Cepstrum Coefficients. In particular the system must recognize a single specified word. Since the audio file I get the MFCCs in a matrix with 12 rows(the MFCCs) and as many columns as the number of voice frames. I make the average of the rows, so I get a vector with only the 12 rows (the ith-row is the average of all ith-MFCCs of all frames). My question is how to train a classifier to detect the word? I have a training set with only positive samples, the MFCCs that i get from several audio file (several registration of the same word).
I make the average of the rows, so I get a vector with only the 12 rows (the ith-row is the average of all ith-MFCCs of all frames).
This is a very bad idea because you lose all information about the word, you need to analyze the whole mfcc sequence, not a part of it
My question is how to train a classifier to detect the word?
The simple form would be a GMM classifier, you can check here:
In more complex form you need to learn more complex model like HMM. You can learn more about HMM from textbook like this one
I have audio records of 4 phonemes (a, e, o, u) from 11 people. I trained an ANN using the data from 10 people, and used the other set for testing. I used 14 LPC coefficients of the first period (20ms) of records as features.
The training matrix
I has 14 rows and 10 columns for each phoneme. So it is 14*40. Since it is a supervised classification problem, I constructed a target matrix
T which is 4*40. It contains ones and zeros where a 1 indicates that the corresponding column in
I is from that class.
The test data matrix contains four columns and 14 rows as it contains 4 phonemes from only one person. Let us call it
Here is the code:
net = newff(I, T, 15);
net = init(net);
net.trainParam.epochs = 10000;
net.trainParam.goal = 0.01;
net = train(net, I, T);
y1 = sim(net, I);
y2 = sim(net, S)
The results are not good even I give the training data as test data (y1).
What is wrong here?
I used 14 LPC coefficients of the first period (20ms) of records as features.
So did you ignore almost all the sound data except first 20ms? It doesn't sound right. You must have calculate an average over all frames at least.
What is wrong here?
You started coding without understanding a theory. Probably you want to read some introduction first. At least this and ideally this
To understand why ANN doesn't work calculate how many parameters are required to map 10 features to 4 classes, then calculate how many training vectors do you have for every parameter. Take into account that for every parameter you need at least 10 samples for initial estimation. That means your training data is not enough.


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Coming out of the closet is a very personal process. You don't force someone you think is in it to come out of it. If you think you're doing the person a favor by doing that, you're wrong. You're being a douche.
Here's how you can be a better friend to someone in your squad, class or office who you suspect is a member of the rainbow community but not quite ready just yet to come out into the open anytime soon.
1. Stop the parinig.
If you think it's funny when you utter remarks hinting at what you're suspecting, it's not. You're being plain cruel. They have valid reasons as to why they keep mum about their sexuality – fear, anxiety, confusion, family issues and other reasons only they would know.
2. Don't taunt them.
"Just for laughs" remarks at their expense is not cool at all. The more you make fun of their not admitting what you believe they are, the more you give them reason not to trust you with any sensitive information and the more you'll drive them far back into the closet.
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3. Don't spread gossip.
When a friend or an acquaintance starts speculating about someone else's sexuality, don't fan the flames by taking part in it. If you get asked the questions, "Bakla ba yun?" or "Tibo ba yun?", you can simply say, "I don't know, but it really doesn't matter." Gently educate them that, for everyone's sake, it's best to leave the person be.
4. Don't ask the "are you?" question.
Ambushing them with the "are you gay?" question especially within earshot of other people is blatant thoughtlessness. You're compelling them to speak about something they're not comfortable speaking to anyone about. That's traumatizing. You must respect their, and in fact, everyone's manner and timeline for doing things that concern nobody but themselves.
5. Don't compare them to others who are out.
What your "closeted" friend is going through is different from the experiences of other people who have already come out, so even if your intention in comparing him or her to others who are out is good, it's actually not helpful. You're just adding to your friend's stress levels. Unsolicited advice is nothing but an annoyance.
6. Earn their trust.
You can open up to your friend about something you're personally struggling with. By sharing your honest thoughts with them, you make them realize that other people are dealing with personal issues that are perhaps as heavy as their own. Make them feel that you trust them and that you're somebody they can trust in return. If they feel your sincerity, they'd be comfortable to open up to you about their innermost thoughts.
7. Make them feel accepted even if they have yet to come out.
Let your friend know your stand against homophobia, but not in a contrived way; otherwise, they'll think that you're doing it just to coax them out of the closet. Just keep it real and natural. The truthfulness of your words and actions will give them the assurance that you accept and support the LGBTQ community and that you're an all-around awesome ally.
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Happy Pride Month!!!
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text: just got an iphone 4, when i plug it into my mac, the mac jumps to iTunes and the speaker on the iphone stops working. it stays not working while i toggle the mute switch. i tried a lot of forums, tried plugging and unplugging the headphones, that didn't help (although sound works fine through the headphones). the only cure seems to be to plug it into the mains charger when it starts working again. anyone encounter this before? the only apps i installed are hipstermatic and a star chart app. no pandora or anything like it. this isn't an end of the world problem because i can fix it but i'd still like to know what the hell is going on. while it's silent, the volume control is on full volume. itunes on the phone doesn't make any sound, neither does the system eg changing the ringtone. thanks for any suggestions.


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To add an audio pole, go over toSpecial:Uploadwhere you will find a type to upload one.
A telephone (quick fortelephone ) is an digital system considered to allow two-approach audio communication.
Per the Reddit lead, FLAC recordsdata can be synced to an iOS machine by way of iCloud push, then accessed via thenew recordsdata application , which is able to allow for native playback of the high-high quality audio recordsdata directly by the machine. If ffmpeg , it might identification mark the primary that Apple has provided help for the popular FLAC discourse format on an iOS device.
That said, the report indicates that FLAC information only work in the information application, not in the Apple Music app. This makes sense, given that would seemingly a more main update to iTunes as properly to support FLAC recordsdata to sync them. still, even when Apple doesnt provide that performance in the future, at least audiophiles seeking to their FLAC collections on Apples units could plague some occupationaround when iOS 11 launches this drop.
How can i exploit media audio?
How am i able to record a streaming audio? 1,077,128questions on Wikianswers Add New web page Edit Edit sourceHistoryTalk zeroThis question is awaiting a solution...Please leave this subject clean until you are answering the question. do not ask questions you already know the answer to. thank you.Retrieved from " " Ad blocker interference detected! Wikia is a unattached-to-fruitfulness website that makes cash from promoting. now we have a made to order expertise for viewers using ad blockers Wikia is just not available if youve made further modifications. remove the custom ad blocker law(s) and the page bestow walk heavily as expected. categories : Un-answered questionsAdd category CancelSave
Make certain you could have an audio enter gadget, akin to a microphone, connected to your pc.
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How are you able to repair an Xbox 36zero that has no audio?
Furniture narrator Stands television Stands Projector Mounts Audio/Video Stands dwelling the stage chairs gentle headphones drone Cancelling headset traditional headphones wi-fi headphones iPod docks speakers iPod Docks Micro systems via ipod docks
How much does an audio engineer originate next to common wage?
Location located on the two6th floor of the stock exchange Tower, Audio Z enjoys gorgeous views of the St. Lawrence River. The studio is simply steps away from a number of the high motels, eating places and boutiques Montreal has to supply. Our important location places you within the coronary heart of Montreals artistic life which is acknowledged around the globe. From music to visible arts, show to bop, show business to museums, Montreal offers all the pieces a inventive mind might want for with out abandonment aside whatsoever cherishes the stomach, as gastronomy is most undoubtedly not left at the rear on this cultural whirlwind.
How do you switch audio from recording to glitter push?
To add an audio pole, go over toSpecial:Uploadwhere you will find a type to upload one.


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|A Collection of Informative and Interesting Articles
|Debt Collections (Advt.)
How to Become an AnesthesiologistBY: Career Advice | Category: Careers | Submitted: 2010-12-23 20:46:17
Article Summary: "An anesthesiologist needs to complete medical education to start work. Read on to know various other aspects of this career..."
The job of an anesthesiologist is very important because this is the person who is mainly involved in monitoring the various aspects of the condition of the patient during the surgery after the administration of the anesthetic. The person has to be very alert and make sure that all the vital signs of the patient who is undergoing the procedure is within the permissible limits. This is because of the fact that if any of the vital signs are not normal, then immediate precautions needs to be taken so that the affected person will be able to become normal.
There are various requirements for a person to become an anesthesiologist. All these should be satisfactorily completed for the individual to become an expert in the selected job.
1. Education: The education is one of the most important aspects of the job. The basic school education should also be focused on the job. Though there is nothing special that needs to be completed during school, the individual has to make sure that he or she takes up Biology in school so that it is easy to do this particular course. Any person who is interested in joining any medical related courses should make sure that they are studying Biology.
2. Degree: The undergraduate medical degree is very important and this too should be a course in Science. The person will benefit much more if the education is in Biology or other science subjects. Though this will be beneficial for the person in learning in medical school and will improve the understanding, the person need not be educated in this to join medical school.
3. Entrance education and qualification: Once the undergraduate education is completed by the individual, the next step is to make sure that the person joins the medical school after completing the entrance exam. Passing this exam qualifies the person to join a medical school.
4. Course completion: The individual needs to complete the medical school which will take at least 4 years for the person. The individual who would like to become an anesthesiologist should only make sure that the medical school is an accredited one. After the completion of the medical school, the individual has to join a residency in anesthesiology. The duration of this course is about 4 years again. This is the specialization that the individual is completing.
5. Certification: The individual also needs to have an certification done. This is got by passing an exam that has both oral as well as the written component. As soon as the person completes this education, it will help the person to be able to become certified to work as an anesthesiologist. This is one of the most important aspects of the person to complete medical education and completing the residency in the selected field. The person will not be able to become a practicing anesthesiologist without the certification.
Though experience is also important in the person to be able to get a lucrative position, it takes time for the person to become completely experienced. There are various methods that can be followed by the person to complete the formalities. Once these are all completed, the person can practice in any hospital as an independent anesthesiologist.
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By leveraging both local and global load balancers, we achieved both server and site resiliency while using a single URL for users to connect to. The GTM resolves a single URL to different IP addresses based on the selected load balancing algorithm and availability of global services. By having the authoritative Windows DNS servers (contoso.com) delegate the URL (pool.contoso.com) to the GTM, users connecting to pool.contoso.com are sent to the appropriate site at the time of DNS resolution. The local server load balancer then gets the connection and load balances it to the appropriate server.See the "DNS and HLB Topology Reference" for the overall architecture and flow use case in the .doc download - http://go.microsoft.com/fwlink/p/?LinkId=211389
The HLBs were configured to monitor the Front End Pool members by using an HTTP or HTTPS monitor, which gives the load balancers the best information about the health and performance of the servers. The HLBs then use this information to load balance the incoming connections to the best local Front End. Using a feature called Feature Priority Activation, we also configured the HLBs to proxy connections to the other central site if all the local Front Ends reached capacity or no longer functioned.
The global server load balancers (GTM) were configured to monitor the HLBs in each site and to direct users to the best performing site. The GTM can be configured to send all users to a specific site in the case of active/standby central sites (as was the case for this test), or load balance users between the sites for active/active deployments. If one site reaches capacity or becomes unavailable, the GTM directs users to the other available site(s).
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In august 1972, 22-year-old houston-born songwriter rodney crowell first arrived in nashville, a passenger in fellow songwriter donivan cowart's 1965 baby blue chevy impala. Crowell, perry d, iv singles 279 magolan, jimmy m singles 279 ross, ryan c singles 279 rosso, timothy m singles 279 ebbole, robert p team 278 rowe, ronald l, ii team 278. Backed primarily by sparse acoustic guitar, nashville 1972 is a lovely country/folk number that recalls crowell's salad days. Peekyou's people search has 99 people named ronald crowell and you can find info, photos, crowell has had five number one singles on hot country songs, . Amazon's choice for rodney crowell close ties 2017 by rodney crowell 48 out of 5 stars 94 streaming singles & eps compilations double albums packaging .
Loretta wrote her first single "i've but she followed it with a tender cover of the rodney crowell composition "till i gain control again . Rodney crowell has always been one of though it had previously been recorded and never released as a single by tracy byrd in 2003 crowell's recording of the . This pin was discovered by vale crowell discover (and save) your own pins on pinterest find this pin and more on new book release by sally singles. Listen to albums and songs from rodney crowell singles & eps play shake your money maker rodney crowell play tobacco road rodney crowell play east houston blues.
Oklahoma head tennis coach nick crowell announced six new additions to sooners reload, crowell signs was the national junior college singles champion in . Rodney crowell's wiki: rodney crowell (born august 7, 1950) is an american musician, known primarily for his work as a singer and songwriter in country music crowell has had five number one singles on hot country songs, all from his 1988 album diamonds & dirt. Rodney crowell - the houston kid - sugar hill - usa.
The outsider is the twelfth studio album by american country music singer rodney crowell it was released on august 16, 2005 via columbia records the album includes the singles "the obscenity prayer" and "say you love me". Gold rackets/pro singles: the tuxedo club: february 14-18, 2019: us open: boston tennis & racket club: march 7-10, 2019: designed by crowell creative . Perhaps no greater exception to this trend was rosanne cash, not only did the album yield three number one singles, her marriage to crowell was beginning . Diamonds & dirt was the first country album to generate five consecutive no 1 singles on the country singles chart ask crowell to cue up any of those tunes now, .
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The Michigan Department of Transportation is warning drivers to be mindful of construction zones following an specially deadly 2016. Spokesperson Nick Schirripa tells WKZO News 17 people were killed in work zone crashes throughout the year. Most were the result of distracted driving.
"Distracted driving is kind of a new phenomenon," Schirripa said. "It is what we have been addressing for the better part of a decade now. People don't quite seem to get it yet."
Schirripa says construction zone fatalities on the road have been rising for a few years now. Schirripa urges drivers to ditch the cell phones when behind the wheel.


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When i was traveling in europe i had the worst time with tinder, my buddy was going off to meet girls and i was alone without more than a match per city, most of who didn't speak english or i've been using dating sites and apps for the past seven years of travel, for making new friends in each place. Given the shortage of young men in post-world war i europe — 10 million soldiers died and 20 million were wounded, many grievously — bernard wonders why any bachelor would want to settle down "you want to have some fun" he asks therese rhetorically, "fine you don't goodbye there are too. In a new series of articles, black women living outside of europe share their views from the other side in our third article, betty byte (not her real name) spells out why she is determined to live in europe a benediction that was given to me a long ago from a beautiful, elegant black female co-worker of mine.
A post on lovin malta that ran over weekend gained some serious traction online after it hit on a sensitive subject with our readers the issue arose from the clear distinction between how beautiful the maltese people think they are when compared to what the rest of europe thinks in a reddit poll, people. Reddit has long led the battle to save net neutrality, as an here in europe we have our own rules and challenges that impact how we get. The dating game can be a minefield, particularly in a foreign country in this week's local list we look at five reasons you should (and. From the worst 'reply all' email fails to the most unexpected sexual experiences people have ever had, reddit brings us all sorts of weird and wonderful anecdotes from all over the world but the latest that's caught our attention is a thread centring around the worst first date stories – specifically, those that.


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