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It's hard for me to imagine living far from the water. Away from the sea. No matter which. The sound of the sea is the only sound which calms me in 100%. But it has to be just the sea sound. No families, windbreaks, dogs, youth, music. Just me and the force of nature. Where you can relax best?
I've been to many beaches. The first which I remember was the beach in Ustka. As I child, I believed that it is the only beach in the world, and that all people come right here. Then I realised that other countries also have access to the sea and, for sure, they have beaches. However, this one in Ustka is one of the most beautiful beaches I have ever been to. Beyond the holiday season of course. Then the beaches should be avoided.
Poland, Ustka
Portugal, Setubal
Morocco, Al-Muhammadijja المُحمدّيه
Morocco, Casablanca الدار البيضاء
Netherlands, Den Hague
Colombia, Tayrona National Natural Park
Colombia, Barú
Malta, Gozo
Romania, Costanta
Poland, Łeba
Greece, Chios
Greece, Deskalopetra
Poland, Gdynia
Poland, Sopot
phot. Adrian Kaszuba
… and you can be sure, that it isn't my last word on that subject.
See you!
translation: Agnieszka Wawiórko


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She has a beautiful ass, I would love to suck that pussy for her.
Nice nice video WOW great head and her body is amazing
This woman is a goddess. de Bonaire would treat her special.
very nice , I actually was about to upload this also....
Good ass fucking. Please don't shave that pretty hair!
wow))) super)
christine young.hooot,niiice
wunderschoen wuerde ich auch gerne
wow hot daddy

subset: commoncrawl


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Wipro Enterprises (P) Limited comprises of two main businesses namely Wipro Consumer Care, primarily into Personal Care products, Lighting solutions & Office furniture and Wipro Infrastructure Engineering business, which provides Hydraulic Solutions for a wide range of applications including Aerospace & Defense and complete end to end solutions in Water and Wastewater treatment for industrial applications.
Wipro Consumer Care (WCCLG), a part of Wipro Enterprises (P) Ltd, is among the fastest growing FMCG businesses in India. Wipro Consumer Care's businesses include Personal wash products, toiletries, personal care products, baby care products, wellness products, electrical wire devices, Domestic and Commercial lighting and Modular office furniture. It has a strong brand presence with significant market share across segments in India, South East Asia and the Middle East.
The acquisition of Unza, Yardley and LD Waxsons has given Wipro Consumer Care a global footprint. Its key brands include Santoor (a Toilet soap brand with extensions in personal care), Chandrika, Glucovita Glucose powder, Northwest Switches, Enchanteur (a female toiletry brand), Romano (a male toiletry brand), Bio Essence (a skincare brand) and Yardley (a luxury toiletry brand).
Wipro Infrastructure Engineering (WIN) spans over four decades in hydraulics. It is today a diversified business in Aerospace, Water Treatment, Additive Manufacturing and Automation Solutions, in addition to Hydraulics business. WIN is amongst the largest independent hydraulic cylinder manufacturers in the world, delivering over one million cylinders to OEMs in different geographies. The Hydraulics Business specializes in designing and manufacturing custom built Hydraulic Cylinders for applications in diverse segments such as Construction & Earthmoving, Material & Cargo Handling, Forestry, Farm & Agriculture, Mining and Truck Tipping. Aerospace business was set up in 2013 and is a solutions provider in Actuators (Cylinder & Piston), Aerostructures, Machining, Sheet Metal, Assembly and Testing.
Wipro Water offers end-to-end solutions in Water and Wastewater treatment for industrial applications, catering to industries such as Oil & Gas, Steel, Power, Pharma & Chemical to name a few. Wipro3D, a leading player in Metal Additive Manufacturing (AM) in India, works with clients from discovery to design, development to deployment of metal AM solutions for demanding applications across Aerospace, Defense, Industrial and Healthcare Segments. The Aerospace business division manufactures Actuators and Precision Engineered Components for Landing Gears and Aero Structure Assemblies. WIN Automation caters to the full-service line of Industrial Automation needs of Indian & ASEAN manufacturing industries.
Wipro Enterprises (P) Limited has two joint ventures namely:


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Murrieta Valley Unified School District - MVUSD is located in Murrieta, California on the southwestern edge of Riverside County. The district's award‑winning academic program educates 23,385 students from transitional kindergarten through high school at 20 campuses. With passage of a modernization bond measure in 2014, the district embarked on a major renovation of its school buildings, including the installation of classroom AV systems.
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Manor Independent School District is a rapidly growing, diverse district near Austin, Texas. It serves over 8,700 students at two high schools, two middle schools, eight elementary schools, and one alternative academy. Located 12 miles northeast of Austin, a hub of the high-tech industry, the district is focused on providing 21st century skills to help students thrive in today's world.
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Lake County Schools in Central Florida operates more than 50 schools and serves more than 40,000 students. The district recently completed a multi-year project that included the installation of Extron AV systems in classrooms at several schools such as Sorrento Elementary, Gray Middle School, Lake Minneola High School, Sawgrass Elementary, and Mount Dora Middle School. Classrooms at Eustis Heights Elementary, the latest school to be upgraded, were outfitted with PlenumVault Digital systems from Extron.
Twenty-first century schools with state-of-the-art audio visual technology are giving Long Beach Unified School District students the edge they will need to compete in a global economy. With the newest Extron WallVault AV systems, students at Nelson Academy and McBride High School can easily see and hear teachers and each other in an interactive, collaborative classroom environment.
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The Forestville Union School District, located in a small Northern California community, is comprised of a single school with 360 students in grades K-8. In November 2010, Forestville successfully passed Measure K, a $5.1 million bond intended for health and safety improvements, modernizing and renovating classrooms, improving energy efficiency, improving technology, and upgrading computers.
North Penn identified a need to upgrade several of its schools and to simplify the management of classroom technology. With only three individuals dedicated to technology and a restricted budget, the district set out to improve the teacher's experience in the classroom and simplify the management of AV technology.
The Indian River School District in Florida serves over 18,000 students in 27 schools. District officials recently sought a way to integrate their existing classroom AV technology to make it easier to use and support. Extron PoleVault and VoiceLift Systems provided all of the capabilities they were seeking and a few additional benefits and capabilities they weren't expecting.
Franklin County Schools, headquartered in Louisburg, North Carolina, has 14 K-12 schools and is experiencing steady growth in student population. Committed to creating a 21st century learning environment, Franklin County administrators recently sought a solution for upgrading their classroom audio/video technology. They chose to install Extron PoleVault Classroom AV Systems and deploy GlobalViewer Enterprise software to streamline system management and support.
Poway Unified School District is located just outside of San Diego, California. The district covers 100 square miles and encompasses 38 schools, two district offices, and more than 34,000 students. With the help of a modernization bond measure, the district began planning a major renovation of its buildings, including the installation of classroom AV systems.
The newly constructed Painted Hills Middle School in Desert Hot Springs, California is the first middle school to be built in the Palm Springs Unified School District in 17 years, so the District wanted to ensure that construction, including the school's AV system, was perfectly executed.
South Ridge School in Culver, Minnesota, which opened its doors in September, 2011, is an ultramodern educational institution that uses such cutting-edge technologies as interactive whiteboards, school-wide Wi-Fi, iPads, and advanced emergency visual notification systems. In keeping with this high-tech approach, the school installed Extron WallVault Systems to handle classroom AV.
Leander Independent School District encompasses more than 200 square miles and is one of the fastest growing school districts in Texas. The district serves approximately 32,000 students on 37 campuses that include technology-enabled classrooms. Managing the audiovisual technologies in these classrooms would be a sizable task for almost any organization. But, Benny Bello, the district's only full-time AV/Network Cabling and Infrastructure Technician, handles it all with the assistance of Extron PoleVault Systems and GlobalViewer Enterprise software.


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After more than 5 years as vice president of Sales & Marketing at C.R. Plastic Products, Ted Scott will be retiring this July. Ted has played a critical role in the development and success of C.R. Plastics in the exciting and growing outdoor furniture industry. Under his strategic leadership and guidance, the company has experienced great growth, expanded sales internationally, and strategically transitioned into multiple sales channels.
"Ted has helped position C.R. Plastic Products for a great future as we continue to fulfill our mission of diverting plastic from oceans and landfills and making beautiful outdoor furniture," said company officials. "We cannot thank Ted enough for his dedication, passion, enthusiasm and motivation over the past several years. He will be greatly missed by the entire C.R. Plastic Products family."


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How to choose Marble mosaics?
Marble mosaics are made from natural stone through a special process and do not themselves incorporate any chemical dyes. The middle marble mosaic preserves the pristine color unique to the stone itself. This kind of natural marble mosaic puts people in the space of unpretentious colors and excellent natural textures. Naturally, they will forget the flashyness in reality. And oh, appreciate the truth and simplicity in this time-fuzzy space.
Because of its small size, marble mosaics can be used to make jigsaws that produce a gradual effect. Marble mosaics are mainly used for wall and floor decoration. Because of the small unit area of the mosaic, the variety of colors, and the endless combination of styles, it can inspire the performance of the designer's modeling and design, and fully display its unique artistic charm and personality temperament. It is widely used in hotels, hotels, bars, stations, swimming pools, entertainment venues, home wall surfaces, art parquets and more.
|Marble mosaic has the advantages of relatively low cost and price, low radioactivity, even smaller than glass, tiles, etc., safe use, and its material characteristics make it easy to process. When you purchasing, you may pay attention to whether the particles are of the same specification and size, and whether the edge of each small particle is neat. Place the single-piece mosaic on the level of the ground to check whether it is flat and whether there is a thick latex layer on the back of the single-piece mosaic. The low water absorption is an important factor in guaranteeing the durability of the mosaic. Therefore, the water absorption rate should also be checked. Drops of water should be placed on the back of the mosaic. The quality of the water droplets spilling out is good, and the quality of the infiltration is inferior.
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The KCNQ2 gene product, Kv7.2, is a subunit of the M-channel, a low-threshold voltage-gated K+ channel that regulates mammalian and human neuronal excitability. Spontaneous mutations one of the KCNQ2 genes cause disorders of neural excitability such as Benign Familial Neonatal Seizures. However there appear to be no reports in which both human KCNQ2 genes are mutated. We therefore asked what happens to M-channel function when both KCNQ2 genes are disrupted. We addressed this using sympathetic neurons isolated from mice in which the KCNQ2 gene was truncated at a position corresponding to the second transmembrane domain of the Kv7.2 protein. Since homozygote KCNQ2−/− mice die postnatally, experiments were largely restricted to neurons from late embryos. Quantitative PCR revealed an absence of KCNQ2 mRNA in ganglia from KCNQ2−/− embryos but 100–120% increase of KCNQ3 and KCNQ5 mRNAs; KCNQ2+/− ganglia showed ∼30% less KCNQ2 mRNA than wild-type (+/+) ganglia but 40–50% more KCNQ3 and KCNQ5 mRNA. Neurons from KCNQ2−/− embryos showed a complete absence of M-current, even after applying the Kv7 channel enhancer, retigabine. Neurons from heterozygote KCNQ2+/− embryos had ∼60% reduced M-current. In contrast, M-currents in neurons from adult KCNQ2+/− mice were no smaller than those in neurons from wild-type mice. Measurements of tetraethylammonium block did not indicate an increased expression of Kv7.5-containing subunits, implying a compensatory increase in Kv7.2 expression from the remaining KCNQ2 gene. We conclude that mouse embryonic M-channels have an absolute requirement for Kv7.2 subunits for functionality, that the reduced M-channel activity in heterozygote KCNQ2+/− mouse embryos results primarily from a gene-dosage effect, and that there is a compensatory increase in Kv7.2 expression in adult mice.
Citation: Robbins J, Passmore GM, Abogadie FC, Reilly JM, Brown DA (2013) Effects of KCNQ2 Gene Truncation on M-Type Kv7 Potassium Currents. PLoS ONE 8(8): e71809. https://doi.org/10.1371/journal.pone.0071809
Editor: Bernard Attali, Sackler Medical School, Tel Aviv University, Israel
Received: March 12, 2013; Accepted: July 2, 2013; Published: August 20, 2013
Copyright: © 2013 Robbins et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants from the UK Medical Research Council (G0500194), The Wellcome Trust (WT081706) and the European Union (LSHM – CT – 2004 – 503038). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: For this work, the authors used transgenic mice with a functional disruption to the KCNQ2 gene, previously described by Watanabe et al (2000: J. Neurochem., 75, 28–33), and supplied to the authors by the Japan Tobacco Inc. By agreement with the latter, the authors' experiments were restricted to sympathetic neurons, and (for experiments in which both KCNQ2 genes were disrupted) further restricted to late-stage mouse embryos because of their non-viability after birth. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.
The human KCNQ2 gene is susceptible to a variety of mutations, many of which give rise to a form of infantile epilepsy (benign familial neonatal seizures, BFNS), and sometimes to forms of peripheral nerve hyperexcitability such as myokymia [1]–[4]. KCNQ2 encodes the potassium channel subunit Kv7.2. This is a subunit of the K+ channel known as the M-channel, in which Kv7.2 is usually associated with the homologous Kv7.3 subunit [5]–[7]. The M-channel is a low-threshold voltage-gated K+ channel that regulates the excitability of many peripheral and central neurons [2], [8]–[10]. Quite small changes to the function of these channels can alter excitability markedly as they are located at electrically critical points in the neuron, such as the axon initial segment and the nodes of Ranvier [11], [12].
A number of different KCNQ2 mutations have been described which variously prevent Kv7.2 expression, inhibit its ability to conduct ions, or alter its voltage sensitivity or kinetics [3], [4], [13], [14]. Interestingly, however, so far only mutations in one of the two KCNQ2 genes appear to have been reported in human studies. This led us to ask what happens to the native M-current when both of the KCNQ2 genes are disrupted. This is important because other subunits of this K+ channel family (Kv7.4 and Kv7.5) are potentially capable of forming functional M-channels, either alone, or in combination with Kv7.3 or with each other [1], [15]–[19].
We have addressed this using sympathetic neurons from a transgenic mouse strain in which the KCNQ2 gene had been truncated at the second transmembrane domain [20]. We elected to study sympathetic neurons because more is known about the composition and properties of native M-channel in these neurons than in any other neuron type. Thus, in the rat sympathetic neuron, while the native channel is primarily composed of Kv7.2 and Kv7.3 subunits (see above), a small proportion of Kv7.2 homomers may be expressed at early developmental stages, and the cells also express abundant Kv7.5 mRNA and protein [7]. Likewise the normal mouse sympathetic M-channel also appears to be composed primarily of Kv7.2 and Kv7.3 subunits [21].
Heterozygotes of the KCNQ-truncated mice survive to adulthood. They are not overtly epileptic but show an increased sensitivity to epileptogenic stimuli [20], In contrast, the homozygote progeny die on postnatal day-1, of pulmonary atelectasis [20]. In consequence, we have been forced to constrain our experiments on homozygote gene-truncated mice to late-stage embryos. Notwithstanding, the results are instructive in revealing an absolute requirement for a functional Kv7.2 subunit in generating the M-current, in spite of the preservation and apparent up-regulation of KCNQ3 and KCNQ5 subunit mRNAs. On the other hand, while neurons from heterozygote embryos showed a partial reduction of M-current, M-current recovered its normal amplitude in neurons from heterozygote adults. This may be relevant to the transient nature of some of the neurological defects resulting from monogenic mutations in the human KCNQ2 gene [1], [3], [4].
Materials and Methods
KCNQ2 gene-disrupted mice
This work was performed under UK Home Office Project Licence 70–6776 (approved July 2008) and University College London Ethics Committee.
We used C57/BL6 mice with a targeted deletion of exons 3–5 in the KCNQ2 gene (encoding the Kv7.2 protein from transmembrane domain II to part of the pore region; [20]). Heterozygote (+/−) gene-truncated male and female pairs (provided by Japan Tobacco, Inc, Yokohama, Japan) were mated to yield wild-type (+/+), heterozygous (+/−) and homozygous (−/−) gene-truncated embryos. Genotyping was performed post-experimentally using PCR analysis on DNA extracted from tail snips with primers described by Watanabe et al [20], hence all experiments were done 'blind'. Because of limited heterozygote reproductive ability (of unknown origin), the colony was regenerated at intervals by back-crossing heterozygote progeny against wild-type C57/BL6 mice. Genetic make-up was confirmed throughout by genotyping. As previously reported [20], homozygote (−/−) embryos were not viable postnatally, but for some experiments 6 week-old heterozygote and age-matched wild-type littermates were used.
Whole superior cervical ganglia were isolated from embryonic day 17 mice, quick-frozen on dry ice and stored at –80°C until RNA extraction. Total RNA was isolated from these tissues using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion following the manufacturer's directions. Reverse transcription was carried out using M-MLV reverse transcriptase (H−), oligo-dT and random hexamers (Promega). Real-time quantitative PCR was performed using the iCyclerTM (Bio-Rad Laboratories).
Mouse primers for KCNQ2 and KCNQ3 were adopted from Watanabe et al [20] and designed to be intron-spanning. They were as follows: G6 (KCNQ2 forward): 5′-actgcctggtacattggctt-3′; m5R (KCNQ2 reverse) 5′-ccccgtagccaatggtcgtc-3′; KQ3-11 (KCNQ3 forward) 5′-caccgtcagaagcactttgag-3′ and KQ3-21 (KCNQ3 reverse) 5′-cctttagtattgctaccacgagg-3′. Primers for mouse KCNQ5 and GAPDH were adopted from Primerbank (http://pga.mgh.harvard.edu/primerbank/) [22], with PrimerBank ID 8132999a1 for KCNQ5 and PrimerBank ID 6679937a2 for GAPDH. They were as follows: KCNQ5 forward: 5′-gtcggcgcaacgtcaagta-3′; KCNQ5 reverse 5′-aaccaaacacaaggagaaaaacg-3′; GAPDH forward 5′-catgttccagtatgactccactc-3′ and GAPDH reverse 5′-ggcctcaccccatttgatgt-3′.
The PCRs were performed using iQTM SYBR Green Supermix with 125 nM primers. To verify specificity of amplification, all PCR products were subjected to a melting curve analysis. To control for the amount of input cDNA, all reactions were normalized to GAPDH. For quantification, relative standard curve methods were used. Results were graphed as relative expression (fold-over) compared with wild type, where wild type was scaled to 1.
Superior cervical ganglion (SCG) were isolated from 17 day old embryos and cultured as described previously [23]. Dissociated SCG neurons from individual embryos were plated on single 35 mm dishes (Nunclon, Denmark), i.e. one dish per embryo. In some experiments, cells were isolated from 6 week-old ("adult") wild-type and heterozygote KCNQ+/− mice.
Perforated patch whole-cell recording
Currents were measured from dissociated SCG neurons after 1–2 days in culture. Experiments were performed blind and genotyping performed subsequently. The extracellular solution contained (in mM): 144 NaCl, 2.5 KCl, 2CaCl2, 0.5 MgCl2, 5 HEPES and 10 glucose, pH adjusted to 7.4 with Tris base. Pipettes were filled with an intracellular solution containing (in mM): 80 K acetate, 30 KCl, 40 HEPES, 3 MgCl2, 3 EGTA, and 1 CaCl2, pH adjusted to 7.4 with NaOH. Amphotericin B was used to perforate the patch [24]. Pipette resistances were 1.5–3.0 MΩ and pipette tips were coated in Sylgard® to reduce capacitance. Recordings were made at room temperature (20–22°C). Mean cell capacitance of all neurons tested was 12.6± SD 6.58 pF (n = 68).
Data acquisition and analysis
Data were acquired and analyzed using pClamp software (version 8.0; Axon Instruments). Currents were recorded using Axopatch (200 series) patch clamp amplifiers, filtered at >0.5 kHz and sampled at 5–10 kHz. IK(M) amplitude and its inhibition by TEA were measured from deactivation relaxations at −50 mV. Results are expressed as mean ± SEM. The program Origin (version 5.0; Microcal Software Inc.) was used for creating figures. All data are expressed as mean ± SEM. Statistical analysis was performed using ANOVA followed by a post-hoc Dunnett's multiple comparison test or a Student's t-test. The level of significance was taken as P<0.05.
Drugs and chemicals
Retigabine was provided by Neurosearch (Ballerup, Denmark) through EU grant LSHM – CT – 2004 – 503038. XE991 and nerve growth factor were purchased from Tocris (Bristol, UK). Tetraethylammonium (TEA) was purchased from Lancaster Synthesis (Morecambe, UK). All other drugs and chemicals, unless stated otherwise, were obtained from Invitrogen (Paisley, UK), Sigma-Aldrich (Gillingham, UK) or BDH Chemicals (Poole, UK).
Kv7 mRNA expression
Expression of Kv7 mRNAs in sympathetic ganglia from wild-type, homozygous KCNQ2−/− and heterozygous KCNQ2+/− mouse embryos was assessed by quantitative PCR as described in Methods. As expected [20]., KCNQ2 mRNA was below detectable limits in ganglia from homozygous gene-truncated embryos, and was reduced by ∼30% from that in wild-type ganglia in heterozygous KCNQ2+/− ganglia (Fig. 1). In contrast, there was a clear increase of 40–50% in both KCNQ3 and KCNQ5 mRNAs in KCNQ+/− ganglia and a greater increase of 100–120% in KCNQ−/− ganglia.
Levels in embryonic wild-type (+/+), heterozygote (+/−) and homozygote (−/−)KCNQ2 gene-truncated mice. Bars show means ± SEM of 6 experiments, using 3 qPCR replicates for each.
M-current is lost in homozygote KCNQ2 gene-disrupted mice
Neurons dissociated from superior cervical sympathetic ganglia isolated from wild-type (wt) (KCNQ2+/+) mouse embryos consistently showed clear M-currents (Fig. 2A, left; 4.5±1.1 pA. pF−1, n = 18, Fig. 2B), comparable to (though smaller than) those previously reported for 6-week postnatal mice [21]. In contrast, no M-current at all (<0.1 pA.pF−1) could be detected in any of 18 neurons from homozygote (−/−) KCNQ2 gene-disrupted embryos (Fig. 2A right; Fig. 2B). Consistent with the loss of outward current and hence increased membrane resistance at depolarized potentials, some of these neurons showed a more prominent 'A-current' on repolarizing from hyperpolarized potentials. Neurons from −/− mice showed no diminution of the delayed rectifier current. Mean current amplitudes at +50 mV were (pA, ±SEM): KCNQ2+/+1740±252 (n = 9); KCNQ2+/−3005±243 (n = 18; significantly different from KCNQ2+/+ P<0.01)); KCNQ2−/−2540±187 (n = 15).
A, representative currents recorded from +/+, +/− and −/− sympathetic neurons. Currents were recorded using a standard deactivation protocol to isolate IK(M) from other voltage-activated currents. The cell was depolarized to −20 mV to pre-activate IK(M), then hyperpolarized for 1 s to deactivate the current. B, mean current density of the deactivation tail currents on stepping from −20 mV to −50 mV recorded from +/+, +/− and −/− neurons (with numbers of neurons in brackets). Note the different scales in A and B (A: pA total current; B: pA.pF−1).
Retigabine does not restore M-current in KCNQ2−/− neurons
In order to check further whether the M-current was fully suppressed in KCNQ2−/− mice, we tested the effect of retigabine. This drug enhances M-current, primarily through a ∼20 mV hyperpolarizing shift of the current-voltage curve [25]. Retigabine enhanced the M-current in neurons from wild-type (+/+) and heterozygote (+/−) mice, giving rise to a 99.7±13.47% (n = 13) and 71.0±10.46% (n = 23) increase respectively in total outward current recorded at −20 mV (Fig. 3, A, B; both significantly (P<0.001) greater than homozygote, see below), but failed to increase the outward current in neurons from homozygote (−/−) gene-disrupted mice (−9.4±4.8%, n = 15).
A. Sample current records. Control, (c); 10µM retigabine, (r). Calibration: pA total current. Note that retigabine slows current deactivation and accelerates current activation as previously observed [25]. B, Mean % increase in standing outward current at −20 mV in +/+, +/− and −/− neurons (with numbers of neurons in brackets). *- indicates significant difference from wild type (P<0.01).
M-currents in neurons from heterozygous KCNQ+/− mouse embryos
Neurons from heterozygote (+/−) embryos showed a significant (∼60%) reduction (P<0.01) in M-current amplitude, as measured from the amplitude of the deactivation current tails (from 4.47±1.09 pA/pF (n = 18) in wild-type to 1.76±0.20 pA/pF (n = 33) in heterozygotes; Fig. 2B). It may be noted that the proportions of neurons from +/+, +/− and −/− mice (randomly selected and genotyped post-experiment) accorded with Mendelian-genetic expectations (+/+, 9; +/−, 17; −/−, 9 ganglia), suggesting that the −/− mutation was not embryonically-lethal, in agreement with the previous report [20].
M-currents in neurons from adult KCNQ2 +/− mice
Since heterozygote KCNQ2+/− mice survive to adulthood, we asked whether the reduction in M-current in these heterozygotes observed in embryonic neurons (Fig. 2) was maintained into adulthood. As shown in Fig. 4A, this appeared not to be the case: in contrast to the embryonic neurons, we could not detect any significant difference between current amplitudes in neurons from KCNQ2+/− adults as compared with those from age-matched wild-type mice (wild-type 6.07±1.44 pA/pF, n = 10; KCNQ+/−6.30±1.46 pA/pF, n = 18), Interestingly, though, retigabine appeared to produce significantly (P<0.005) less enhancement (+99.1±4.80%, n = 10) of the KCNQ2+/− current than of the wild-type current (+133±9.07%, n = 7) (Fig. 4B).
A: mean M-current density (pA.pF−1) in wild-type (+/+) and KCNQ2+/− neurons. B: current enhancement by 10 µM retigabine, measured as % increase in standing outward current at −20 mV (see Fig. 3). C: mean % inhibition of wild-type (+/+) and KCNQ2+/− M-current by increasing concentrations of tetraethylammonium (TEA, mM). Vertical bars: SEM; numbers of neurons tested in brackets. * – indicates significant difference from wild type (P<0.005).
Since retigabine appears to have far less effect on Kv7.5 channels than on Kv7.2 or Kv7.3 channels [26], this raises the possibility that the maintained M-current was due to a compensatory increase in the contribution of Kv7.5 or Kv7.3/7.5 channels to the recorded current. We assessed this using tetraethylammonium (TEA), because Kv7.2-containing channels have a much higher sensitivity to TEA than Kv7.5-containing channels (IC50 values, mM: Kv7.2 homomers, 0.12–0.17; Kv7.2/7.3, 3.8; Kv7.5, 71; Kv7.3/7.5, >200; [5], [7], [17], [27]. However, the overall TEA sensitivity of the neurons from adult KCNQ+/− mice was not significantly different from that of the wild-type neuron current (Fig. 4C). Interestingly, though, current inhibition in all of these adult mouse neurons was only partial, even at 30 mM TEA (wild-type: 68.3±5.8%, n = 7; KCNQ2+/−: 57.0±4.4%, n = 8). Had the current been generated solely by Kv7.2/3 heteromers, or by Kv7.2 homomers, or a mixture of the two, 30 mM TEA should have inhibited ≥89% of the current. Thus, allowing for up to 10% current through unblocked Kv7.2-containing channels, some 22–33% of the residual unblocked current might have been carried by channels composed of Kv7.5 or Kv7.3/7.5 channels. Notwithstanding, there was no evidence for a substantial substitution of Kv7.5 for Kv7.2 in the KCNQ2+/− neurons, and certainly not sufficient to compensate for the ∼60% reduction in current observed at the embryonic stage (Fig. 2).
The principal point established in these experiments is that the presence of the Kv7.2 subunit is an absolute requirement for the expression of functional M-channels in the sympathetic neurons of late-embryo mice. Thus, no M-current could be detected in sympathetic neurons from mouse embryos in which both KCNQ2 genes had been truncated (KCNQ2−/− mice), even after M-current amplification with retigabine. If also true for the many key central neurons expressing Kv7.2 subunits (and presumably M-channels containing this subunit) [28], this suggests that severe double-KCNQ2 gene mutations in humans might be embryologically-lethal, perhaps explaining the monogenic nature of reported human KCNQ2 mutational diseases.
This double KCNQ2 gene truncation did not prevent KCNQ3 or KCNQ5 gene expression: indeed, mRNA levels for these genes were approximately doubled. While we could not assess the respective membrane protein levels, it seems reasonable to assume that some at least of this mRNA was translated into protein. Notwithstanding, the complete absence of M-current in the KCNQ−/− mice implies that any expressed Kv7.3 and Kv7.5 subunits were unable to form a significant number of functional channels in either homomeric or heteromeric form when both of the KCNQ2 genes were truncated. Kv7.3 homomers generate very small currents [29]–[31], largely because expressed channels are non-conductive [32]. However, this does not apply to Kv7.5 homomers, or Kv7.3/7.5 heteromers, which can generate good currents [16], [17], [33]. A multiplicity of factors affect the surface expression and conductance state of Kv7 subunits [34] and we cannot speculate which, if any, prevent efficient M-channel generation by Kv7.3 and Kv7.5 subunits in ganglionic neurons devoid of Kv7.2 subunits.
Neurons from mice in which only one of the KCNQ2 genes had been truncated (KCNQ+/− mice) showed a partial (∼60%) reduction in M-current amplitude. This was accompanied by a ∼30% reduction in KCNQ2 mRNA expression. Bearing in mind the uncertainty about how quantitatively the latter translates to a change in membrane protein expression, the most parsimonious explanation is that the reduced current results from a gene-dosage effect (haploinsufficiency), as seen with the pore-defective Kv7.2(Y284C) mutation responsible for one form of human BFNS [35]. Although we cannot exclude a minor additional dominant-negative effect of the truncated N-terminal residue on the expression of functional heteromeric Kv7.2/7.3 channels, this seems unlikely since this protein fragment lacks the Kv7 C-terminal assembly domain [36].
Interestingly, this partial suppression of M-channel activity in neurons from embryonic KCNQ+/− mice did not appear to be preserved into the adult stage of the heterozygotes. This accords with observations on hippocampal neuron M-currents in adult mice expressing the BFNS-replicating KCNQ2 mutation A306T, where M-current amplitudes in neurons from heterozygote A306T+/− were unchanged compared with those from wild-type mice, even though the former showed a reduced seizure threshold [37]. In contrast, hippocampal neuron M-currents were significantly reduced by ∼20–30% in heterozygote mice expressing a spontaneous partial C-terminal deletion in one KCNQ2 gene [38]. We cannot directly explain this difference, but would note that the KCNQ C-terminus is the site for most M-current regulatory mechanisms [39], which might be selectively disrupted by a partial deletion.
In our experiments, the maintained M-current in sympathetic neurons from the adult heterozygote mice could not be accounted for by an increased functional expression of Kv7.5 or Kv7.3/7.5 channels, since the TEA sensitivity of the KCNQ+/− M-current was not significantly different from that of the normal wild-type mouse neuron current. Presumably, therefore, restoration of the current in adult KCNQ2 gene-truncated transgenic mice results primarily from a compensatory increase in Kv7.2/7.3 expression. Though these KCNQ+/− mice are not epileptic, their neurons are hyper-excitable in so far that the mice are more sensitive to epileptogenic agents [20]. This neuronal hyperactivity might augment KCNQ transcription, since stimulation of rat or mouse sympathetic neurons in vitro has been reported to induce a transcription-dependent increase of functional M-channel expression [40]. Although other mechanisms, such as increased GABAergic inhibition, have been suggested [4], a comparable restoration of the M-current in human instances of monogenic KCNQ2 mutations might well contribute to the developmental recovery from the transient epilepsies seen in many cases of BFNS [1], [3].
We thank Dr. Hitoshi Sasai (Tago Clinic, Shizuoka, Japan) for supplying the gene-truncated mice for breeding, Marc Lind and Tina O'Mahony (UCL Biological Services) for expert mouse breeding and maintenance, and Stuart Martin (Department of Neuroscience, Physiology and Pharmacology, UCL) for efficient genotyping.
Author Contributions
Conceived and designed the experiments: JR GMP DAB. Performed the experiments: JR GMP FCA JMR. Analyzed the data: JR GMP FCA JMR. Wrote the paper: JR GMP DAB.
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text:
Today at the church ,all of the teens include me also.
Uncle Yew Mun is the teacher for the Teens Talk.
Now he will go to Australia.
Hope the God will be bless him and his family members.
Those Angles will also besides with he and his family.
Hope his family will be happy and enjoy the life at there.
All things must truth in God,He will help us and protect us.
Bless Uncle Yew Mun and his family have a good health.


text:
UVU Women's Hockey has received a commitment from forward Kelli Louie for the team's 2018-19 inaugural season.
Louie is from Salt Lake City, Utah and most recently played with the Wasatch Wings and Utah Howl. She also has experience in the NCAA with Utica. She will sport #7 and will be a grad student at UVU.
UVU Women's Hockey is excited to have Kelli on the team and can't wait to see her skill on the ice sporting the Wolverine.


text:
Few things are as frustrating as losing a domain name to a competitor or finding that someone else has registered your business name or trademark as a domain name. Adding to the frustration, the domain name registrant may demand a significant amount of money in response to a request to purchase or transfer the domain name. However, trademark owners who find themselves in this type of predicament may have legal options available. If a domain name registrant is "cybersquatting," the trademark owner may compel the transfer of the domain name by filing a complaint under the Uniform Domain Name Dispute Resolution Policy (UDRP) or by bringing an action in court under the Anticybersquatting Consumer Protection Act (ACPA).
What Is Cybersquatting?
Cybersquatting refers to the act of registering domain names of existing trademarks for the purpose of reselling them for a profit. To prove cybersquatting, a trademark owner must show:
- that the business owner owns a trademark (either registered or unregistered) that is confusingly similar to the domain name;
- that the domain name registrant has no legitimate right or interest in the domain name; and
- that the domain name registrant registered the domain name in bad faith.
What does "confusingly similar" mean?
If the public might confuse the domain name with the trademark, then the domain name and the trademark are confusingly similar. In assessing whether a domain name and a mark are confusingly similar, perform a side-by-side comparison of the domain name and the textual components of the trademark to determine whether the trademark is recognizable within the domain name. If the domain name uses the entire trademark or a substantial part of it, the domain name is likely confusingly similar to the trademark.
A trademark owner need not have registered the trademark with the U.S. Patent and Trademark Office (USPTO) to show that it is confusingly similar to the domain name. Although a trademark has not been filed with the USPTO, an owner may nonetheless have legal rights to that trademark and be able to enforce those rights against a cybersquatter. These rights are called common law rights. Common law rights in a trademark arise when a person uses a unique mark in connection with a business but does not register the mark with either the state or federal trademark office. Though common law rights are more limited than the rights that come with formal registration, a common law trademark still confers upon the trademark owner the right to enforce its trademark against a cybersquatter.
What is a legitimate right or interest?
A domain name registrant must hold the domain name for a legitimate commercial or personal purpose. Registering a domain name with the intent to sell it for profit is not a legitimate right or interest. Often, holders of domain name registrations who merely seek to sell and profit on the domain name will not have a functioning website at the domain address. A site that is blank, contains only advertisements, or displays a "for sale" statement, is likely owned by someone who does not have a legitimate right or interest.
What is bad faith?
Bad faith means that the domain name registrant registered the name primarily to keep the business or trademark owner from using that domain name. In looking for evidence of bad faith, determine whether the domain name registrant holds the domain name for the purpose of selling, renting, or transferring the domain name to the trademark owner or to a competitor of the trademark owner for a profit. Again, if the domain address leads to a blank page or to a "for sale" page, the owner likely intends to sell the name to you or a competitor for profit. Additionally, if a competitor purchases the domain to disrupt your business or to divert traffic from your business, there is likely bad faith.
Why Is Cybersquatting Illegal?
Trademark owners have exclusive legal rights to their intellectual property. Rules against cybersquatting prevent domain name registrants from profiting from another's trademark. The laws ultimately protect businesses and individuals from being exploited for monetary gain.
How Do I Acquire a Domain Name from a Cybersquatter?
If you believe that someone is cybersquatting in violation of your trademark, you can either file a complaint under the UDRP or you can file an action in court. If you are successful, the domain name will automatically transfer to you at no cost other than filing and attorney's fees. Filing a complaint under the UDRP is cheaper, quicker, and easier than filing an action in court. However, the only remedy available in a UDRP proceeding is the compelled transfer of the domain name. Monetary damages and reimbursement for fees are not available under the UDRP. If you file an action in court under the ACPA, the court may award monetary damages for any injury your business has sustained due to the actions of the cybersquatter. Unless your business has sustained substantial injuries, filing under the UDRP is generally more cost-effective because court filings are significantly more expensive and time consuming.
If you believe you are the target of a cybersquatter, there may be legal remedies available to you. A consultation with an experienced attorney can help you determine what rights you may have.
Kuzmich & Associates is located in Tempe, Arizona. We assist many businesses throughout the state with a variety of legal issues, including employment contracts, leases, contract review and business transactions. Need an experienced business law attorney? Contact us.


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Maintenance Program
Every home electrical system needs annual service, Our PowerClub maintenance agreement will help keep your home's electrical system running at its optimum performance levels.
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- Glendale
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Phoenix, AZ 85027
Keep In
About Mister Sparky
Mister Sparky was started in 1996 in Atlanta, Georgia. That location is now the leading residential electrical service and repair company in the Eastern U.S. and one of the biggest in North America. The name "Mister Sparky" comes from the common use of the term "sparky" or "sparks," meaning electricians, in company founder Patrick Kennedy's native Ireland. The company has an impeccable record for delivering fast, on-time service, accurate upfront pricing and an overall pleasant experience for customers.
At Mister Sparky we differentiate ourselves from all other electrical companies by our unique message to the marketplace: Mister Sparky – "America's On-Time Electrician."
Our motto is: We're On Time, You'll See, Or The Repair Is Free. And we mean it.
In fact, we are the "On-Time" contractors in the electrical industry and we champion it in the marketplace. With over 86 locations nationwide, the Mister Sparky franchise has managed to uphold its reputation by providing honest, impeccable service that simply gets the job done.
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24/7 Emergency Service


text:
What a day...
*Internet is an "addiction"~*
Woke up late today... 8.10am leh. Rushed out in like 10mins time haha. OOCL gave us each 1 box of mandarin oranges and 1 packet of Bee Cheng Hiang bah kuah each haha~ mum say altogether oso cost 50+bucks le la! Sadly, I dun have red packet~ only perms staffs hav~ haiz! 100bucks each neh... Evelyn was joking ask QW give me half of hers haha. Oh yeah, I accidentally knocked over QW's water in the morning... then made her whole table wet... Evelyn was saying: "Gd la, time to do spring cleaning oso Ah Wei~" LOL.
*Yeah~ Haslin promise to lend me "Camp Rock" DVD!*
*Stella... my real mum so much better and prettier as a person ok!?*
Shermeen was such a saddist today... curse my lappy piang! We went for lunch together, then she was "black face" throughout... and she said: "I'm very unhappy with ur friend!" QW oso very bu shuang with her la. Think she old liao... I mean yrs of exp so wat? You come to a new company of cos everything have to learn again. And QW they all already told her umpteen times how to do, stubborn Shermeen somehow still dun learn leh.
Night gt tuition with Brendan. He can't concentrate at all. Sometimes, I really feel like quitting his tuition leh~ but he's quite a "sticky" boy haha. Dunno how, think one day sure his mum or I can't stand, then we really gonna say bye-bye le. Plus nw I have a few tuitions... making my personal time table kinda packed, if I really get into Uni, sure got to let go a bit de.
Okie... maybe on a happier note: my DBSK socks and calendar'09 have arrived!It's of really gd quality~ love it!
These socks are simply cute and lovely! I can't bear to wear them even! Love them a lot!


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The Bodo-Garo subgroup of languages, spoken in Assam and Meghalaya, Northeast India, falls under the larger subgroup Bodo-Koch with Tibeto-Burman. Despite controversies surrounding the classification of other Tibeto-Burman languages, Bodo-Garo is a well defined subgroup that is not disputed. Within Bodo-Garo, there are two major branches, Bodo and Garo. Garo consists of the various dialects of Garo spoken in the Garo Hills in the west of Meghalaya.
Read more about Tiwa.


text:
Infinity Technology Services Releases High Speed IDS
Infinity Technology Services has announced shipment of the INDESYS Network Intrusion Detection System. The company says the product is capable of full duplex capture at 100Mbps.
By Enterprise Networking Planet Staff | Posted Apr 22, 2002
Page of | Back to Page 1
EUC with HCI: Why It Matters
Components of the INDESYS package include:
- INDESYS sensors: hardware appliances designed to monitor network traffic. The 1u form-factor units are built around Pentium III 1.26Ghz processors with 512MB of RAM.
- the INDESYS Management Server, a Linux-based application that manages collected data from multiple sensors.
- The Operator Console, a Windows 2000-based application capable of monitoring multiple sensors.
Features listed by ITS include:
- Fast-Ethernet (wire speed) full duplex data capture at 100Mbps. Wire speed capture means that packets are captured at the network interface physical media speed. On Fast Ethernet, this translates to 100Mbps, without dropping or losing any packets.
- The sensor makes use of two (2) data capture interfaces,
allowing a few options as to the integration to the network being
monitored. The sensor supports:
- A single interface connected to a network switch configured to provide port mirroring
- Two interfaces connected to separate network switches configured to provide port mirroring
- Two interfaces connected to a load server balancing switch
- Two interfaces connected to a TAP (Test Access Port).
- Fragmented packets are re-assembled and scanned to ensure that no malicious activity is occurring on the network
INDESYS is offered in two different versions:
- INDESYS 100 provides a max throughput of up to 200 Mbps.
- Infinity plans to release INDESYS 1000 in early July. The company reports this model will provide a max throughput of up to 2 Gbps.
Additional information may be found at Infinity's web site.


text:
Explore McMaster
Welcome to the Department of Surgery at McMaster University. Our large faculty contributes extensively to our unique ability to provide specialty care for Hamilton and the surrounding communities through St. Joseph's Healthcare and the Hamilton Health Sciences. Committed to integrating innovative clinical care, world-leading research, and outstanding educational resources, the Department of Surgery aspires to continue to be a leading department in academic surgery both nationally and internationally.
Stay current with the most recent updates from the Chair, the trending news from our Department, and the events you won't want to miss!
A database of helpful links, documents, and forms for McMaster University Department of Surgery faculty and staff.
Progreston Falls is a beautiful, serene place. Located just outside of rural Carlisle, Ontario, the private parkland is home to a harmony of plants, animals, and natural wonder. A tributary of Bronte Creek cuts through the area, spilling over a 54-foot-wide cliff that forms a classic wide-terraced curtain waterfall that many locals routinely gawk over. But on July 21, 2016, that serenity was juxtaposed with horror.
Sixty-three-year-old Ben Paavilainen was called to Progreston Dam for a routine construction project. During his initial inspection, however, a huge concrete wall of the dam came loose and crushed him, pinning his body to the riverbed below.
In incredible time, police, fire, and paramedics were all on scene to attempt to rescue Paavilainen. Despite the fleet of emergency responders, however, the group was no match for the 25-ton concrete wall. Paavilainen was stuck.
Stuck as he was, however, he was alive and well — or as well as he could have been given the situation. The plan was, if the slab could not be removed from Paavilainen, he would be removed from it. The first responders called the emergency unit at Hamilton General Hospital to see if they could get some surgeons on scene to help amputate a trapped civilian's legs.
Dr. Niv Sne, a McMaster University trauma surgeon, clearly remembers receiving the call from the emergency physician who apprised him of the situation.
"The natural response when you learn of something like this is to start preparing the operating room," he says. "But, for the first time in more than 15 years of doing this, they said, 'no, no… you're going out there.' They told me that the emergency responders were concerned that there would be no way to pull the concrete slab off of the patient, and the poor gentleman would require bilateral lower-limb amputation."
Dr. Sne's first order of business was to decide who he'd bring along with him — enter Dr. John Harlock.
"Dr. Harlock is an esteemed vascular surgeon, and I called him without hesitation and asked him what he was doing," Dr. Sne recalls. "He goes, 'Not much. Just about to eat lunch. What's going on?' I told him, 'Put the lunch aside — we're going out to the field to do a bilateral lower-limb amputation.' His first reaction was, 'Nah — you're kidding.' But I sadly wasn't and we were on our way to the scene before either of us knew it."
Upon their arrival, both surgeons were awestruck by the extreme juxtaposition of the scene.
"It was kind of surreal," Dr. Harlock recalls. "I remember there was a family of ducks swimming peacefully on one side of the river and a serious trauma unfolding on the other. It was crazy."
"It was totally picturesque," Dr. Sne adds. "A beautiful site host to a horrible incident."
The day was exceptionally hot, and, dressed in OR scrubs and running shoes, the two surgeons were ill prepared for the journey down to the foot of Progreston Dam. It's a steep climb down along a path lined with jagged, moving rocks — a path tough enough to descend in proper hiking gear. But with the guidance of a few firefighters, the two surgeons were on scene and ready to operate.
For both doctors, the accident was far worse than imagined. Not only was the concrete slab crushing more of the patient than expected, but there was also another critical detail that neither Sne or Harlock were prepared for.
"He was awake," Dr. Harlock says. "Awake. Aware. Alert. Talking. Moaning and groaning. He was breathing on his own, and maintaining blood pressure."
Performing a bilateral lower-limb amputation on a sedated patient in the comfort of a hospital operating room with the help of power tools is one thing for two experienced surgeons like Drs. Sne and Harlock. But, performing a surgery as complex as that on a patient who's completely alert, lying in a river, and pinned to the earth by 25 tons of concrete using only a string-saw — the kind of saw you'd use
to cut wood in a forest — was a completely different story.
Dr. Sne described the situation as "organized chaos." He says that the first responders did an outstanding job of controlling the situation, but there were still far too many factors preventing them from simply swooping in and performing surgery.
As such, the first order of business for the surgeons was to intubate the patient.
"The OR can be quite chaotic," explains Dr. Harlock. "People die. They come in bleeding from ruptured aneurisms or gun-shot wounds, but, even then, it's still relatively controlled chaos. It's my workplace. It's what I'm used to. Performing any kind of surgery in a riverbed is a lot different. You're out of your comfort zone. So, when we got the patient intubated, things felt a lot more controlled and we were able to get to work."
And get to work they did. The plan was to use a scalpel to cut through his muscles and nerves and use the string-saw to cut through the bone. Dr. Sne looked after the initial intubation and took control of the bleeding. Dr. Harlock cut down on the patient's thigh to place clamps on his blood vessels. Together, they ensured his vitals were okay, that he was properly sedated, and that he was well looked after.
"I knew then and there that it wasn't going to be a nice amputation, being so high up on the legs," Dr. Harlock says. "It would have been tough to get proper tissue coverage, and I was just going to do a guillotine cut straight down and do my best to repair it when we got the patient back to the hospital."
Notice Dr. Harlock's use of past tense. "Wasn't." "Would have." "Was." Just as the surgeons were about to perform the amputation, they got word that a crane was approaching to help lift the slab off of the patient — music to their ears.
In a few short minutes, the crane was on site, attaching its pulley to the slab. Even then, though, there was more uncertainty than relief.
"My mind was racing," Dr. Harlock recalls. "Is he bleeding out under the concrete? What state were his legs in? Would the concrete fall further onto his body if the crane slipped up at all? The whole situation was really tense."
With the fire chief directing the crane, Dr. Sne, Dr. Harlock, and the rest of the first responders were ordered to temporarily vacate the area due to the very real risk of the slab tipping onto somebody else. With all its might, the crane managed to lift the 25-ton slab about six inches off of the patient — just high enough for him to be slid out from underneath.
To both surgeons' surprise, the patient's lower limbs were in an okay state, all things considered.
"Because it was a true crush injury, all of his blood vessels were sent into spasm, meaning there wasn't as much bleeding as you'd expect," Dr. Sne explains. "We got him on a stretcher, put a tourniquet on his legs, and got him ready for transport to the hospital."
At this point, Dr. Sne says there was a lot of communication with the hospital. They already had an operating room set-up; all they had to do now was get the patient there alive.
Dr. Sne travelled back to the hospital with the patient and the paramedics by helicopter, while Dr. Harlock went back in an ambulance with a police escort, which arrived at the hospital five minutes before the helicopter did.
"When the patient arrived, we bypassed emergency and went straight to the operating room," Dr. Harlock says. "We had people from orthopaedics waiting there — people from plastics, too. Mix in Dr. Sne from general and myself from vascular and it was quite a team effort."
After a lengthy operation, both legs were salvaged. Although he suffered fractures in several locations, the patient didn't require any significant amputation, except for a small bit of work on his right foot. Considering what was originally planned, this was a major win for both the patient and for the surgeons.
Alotgether, the whole excursion was just a few hours long. But with so much uncertainty and so many uncontrollable factors, the day seemed like an eternity for Drs. Sne and Harlock (and surely for the patient, too).
"In hindsight, there were a lot of things Dr. Harlock and I did not think about," Dr. Sne says. "We now have a lot more protocols in place, in case something like this ever happens again. We got lucky that it was during daytime, and not the pitch black of night. We were lucky it was in the summer, and not the dead of winter. All things considered, I'm sure the circumstances could have been a lot different if the stars weren't so well aligned."
Lucky or not, the truth is Drs. Sne and Harlock acted heroically that fateful day at Progreston Falls. They were calm in the face of chaos, and decisive in the face of urgency. If you ask them, they'll tell you that they were just doing their jobs. Modest, yes, but also true — they were just doing their jobs. And that's what's so amazing about it all. Every day, Drs. Sne and Harlock help people in need and save lives. For that and more, we're proud to call them our Every Day Heroes.
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When you plan to learn about how much biotin to take for hair growth in your daily life, you should keep reading this article. The average dosage of biotin should be around 500 – 1,000 mcg daily. However, you need to consider some important details before you determine the right Biotin dosage.
Biotin can help you grow your hair, especially when it is consumed in the right amount. You need to buy the best biotin supplement on the marketplace these days. This supplement is very useful to help you grow your hair rapidly. Biotin can help you have healthy, long, and beautiful hair easily.
Recommended Dosage on How Much Biotin to Take for Hair Growth
a. Biotin Dosage for Adults
Adults and children may have different Biotin daily requirements. Most normal adults usually require about 1,000 mcg daily, in order to grow their hair quickly. This amount is considered as a safe dosage for most adults today.
b. Biotin Requirements for Kids
If you want to give Biotin for your children, you need to take a look at the safe amount for them. Most children or kids should consume about 500 mcg every day. They should not take too much Biotin in their daily life, in order to maintain their digestive system.
Does Biotin Work for Hair Growth?
After you read this article, you should understand that you are able to consume Biotin in your daily life. This ingredient is very useful to help you grow your own healthy hair easily. However, you need to follow the right dosage for consuming this ingredient.
When it is necessary, you can also consult with professional doctors. They can give you the right dosage of Biotin that you should consume regularly. They can help you determine how much Biotin to take for hair growth daily.


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■ Make this recipe
What is it about?
■ Make this recipe!
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■ Make this recipe!
rafters is a recipe sharing service that treats #Make, #Fix, #Grow, #Eat as the main theme.
From device production, cultivation method of tomato, from do-it-yourself carpenter to today's dinner
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You can also send smiles to posted recipes and activities during production activities.
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■ Features of rafters
- You can post recipes with high degrees of freedom with functions specialized for writing recipes
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As usual, the IHSA's state bass finals at Carlyle Lake included tangential catches, such as the 15-pound catfish caught on a crankbait by Minooka's Wyatt Pazdro.
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Age-related macular degeneration (AMD) is a problem with the retina in the eye. It occurs when the central retina, called the macula, is damaged. With AMD, patients lose their central vision, but the peripheral, or side vision, is not affected.
AMD is very common and is actually the leading cause of vision loss in people 50 years or older.
There are two types of AMD:
Many people don't realize they have AMD until their vision is very blurry. This is why it is important to have regular visits to an ophthalmologist. He or she can look for early signs of AMD before you have any vision problems.


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Tuesday September 6, 2016 10:00 AM to 11:00 AM Please join my meeting from your computer, tablet or smartphone. https://global.gotomeeting.com/join/193148277 You can also dial in using your phone. United States : +1 (571) 317-3122 Access Code: 193-148-277 First GoToMeeting? Try a test session: http://help.citrix.com/getready 0 signed up so far. Event date is past
One With Courage Report Child Abuse in Colorado If you suspect a child is being abused, be the One With Courage and contact your local County Department of Human Services or Law Enforcement. If the situation is an emergency, or if the abuse is suspected outside of normal business hours, call 9-1-1. Below is contact information
Click here for a larger image Counties Served as of February 2018 CACs are located throughout the State of Colorado Blue Sky Bridge Child and Family Advocacy Program Gina Earles, Executive Director 2617 Iris Hollow Place Boulder, CO 80304 303-444-1388 303-444-2045 fax firstname.lastname@example.com www.blueskybridge.org Denver Children's Advocacy Center Gizane Indart, Executive Director 2149 Federal Blvd. Denver,


text: Stay warm and stylish in these comfy boots! The features include a faux suede upper with a round closed toe, slip on design, faux fur trim, straps with toggle beaded detailing, smooth lining, and cushioned footbed. Approximately 14 inch circumference and 11 inch shaft. Style: shoes-boots-sdi-guru-10beige This item is FINAL SALE and is not eligible for return or exchanges.


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It's a little-known fact that a private jet charter in and out of the Houston area isn't necessarily as expensive as one might think. It's actually possible to board your own private jet at a fraction of the typical cost, be it for business or for pleasure in order to get the luxury experience of charter flights. You may wonder how. It's simply by means of using empty leg flights.
What are empty leg flights?
Whenever an aircraft's flight doesn't originate from the airport it's currently in for whatever reason, it needs to be repositioned to the airfield from which its scheduled flight will be taking off. This repositioned flight will usually contain no passengers save for the pilots at the helm; this is referred to as an empty leg flight.
How You May Benefit
You may be able to catch an opening aboard an empty leg flight at a dramatically lower cost by booking a spot on a plane whose repositioned route departs from your location heading towards your intended destination. Throughout Houston and other nearby destinations, this makes for a great way to enjoy charter jet flying without breaking the bank.
One Way Charter Flights
For the most part, private jet charter flights will return to their base airport without any passengers on board. Should you be located within the area where the jet begins its return flight, and have as your destination the plane's point of origin, you might have the opportunity to enjoy a heavily subsidized luxury charter jet flight. Of course, you might have to be a bit flexible with regard to the make and model of the jet plane in question, but it's definitely worth it.
Empty Leg Flight Bookings
There's a constant availability of these flights available to you so long as you're not too picky as to the type of jet you'd like. All you need to do is let us know what your destination and point of origin are, and we will inform you of upcoming openings that might suit your needs. Should you find one of these suitable, we'll let you know what the total cost of the trip will be coming to, a figure that's sure to put a smile on your face.
Saving Costs
The beauty of the empty leg flight arrangement, and why it works to the advantage of everyone, is that we get to subsidize the cost of bringing our plane back home while splitting some of the cost savings with you, the client. That's why the flight experience, while cheaper, is of the exact same standard of luxury and all-around quality you would get on any other regular charter jet flight.
Empty Leg Flights for Excursions
A great way to make use of these flights is for the occasional weekend excursion or adventurous getaway. A private jet charter might just be what you need to make your leisurely trip the experience of a lifetime.
Light jets or Heavy Jets?
The best part of taking an empty leg flight might just be the option to choose between light jets and heavy jets for your charter flight. While light jets are perfect for single party or small group hires, heavy jets amply accommodate larger parties such as travel groups and corporate travel groups. With our large fleet of jets, (link here) you will have a wide selection to choose from.
Domestic Empty Leg Flights
A greater percentage of empty leg flight availability falls under the category of a domestic flight to and from Houston and within the U.S. You can literally go city hopping or stop over in the next state, all according to your preference.
International Empty Leg Flights
Though less commonplace as the domestic kind, you may also come across empty leg flights going across the border into Canada or Mexico, or even longer-range intercontinental destinations, all depending upon their availability. There's really no better way to see the world. Make a consultation with us beforehand so we can see how to help you make your international chartered private jet adventure a reality.


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1. Look at the parts and specifications of forklift accessories.
Most forklift accessories have a specific part number and technical parameters. When purchasing electrical system accessories, attention should be paid to checking whether the voltage and power of the replacement parts are the same; when purchasing the driving belt of forklift engine, attention should be paid to the type and length width.
2, see whether the logo of the outer package is complete.
The quality of the forklift truck accessories is relatively good, and the handwriting on the box is clear and the overprint color is bright. Some important components such as generator, starter, hydraulic pump, etc. are also equipped with operation instructions, certificates and inspector's stamps to guide users to use maintenance methods correctly. Therefore, when purchasing, we should recognize them carefully so as not to buy fake and forklift truck accessories.
3. Look at the shape and size of forklift accessories.
Some forklift accessories are prone to deformation due to manufacturers' manufacturing, logistics, transportation and improper storage. When inspecting, the axle parts can be rolled around the glass plate to see if there is light leakage at the joint between the parts and the glass plate to judge whether they are bent or not; when choosing steel sheets or friction sheets of forklift clutch driven disc, the steel sheets and friction sheets can be selected in front of the eyes to observe whether they are warped or not; when choosing sealing parts such as forklift oil seal, the skeleton of the forklift truck can be used to determine whether they are bent or not. The end face of oil seal should be round and can be fitted with flat glass without warping. The outer edge of oil seal without skeleton should be rectified and deformed by hand grip, so that it can be restored to its original state after loosening hands. When choosing all kinds of liners, we should also pay attention to checking the geometric size and shape of accessories with professional measuring tools.
4, see whether the joint parts of forklift accessories are smooth.
During the handling and storage of forklift truck parts, or because of vibration and turbulence, burrs, indentations, damages or cracks often occur in the joint parts to affect the installation and use of forklift truck parts. This should be checked when purchasing or inspecting goods.
5, see whether there is rust on the surface of forklift accessories.
The surface of qualified fittings has both a certain precision and a bright finish. The more important the fittings are, the higher the accuracy is, and the more strict the packaging is, the more rust-proof and anti-corrosion. When purchasing or inspecting goods, attention should be paid to inspection. If rust spots, mildew spots, cracks in rubber parts, loss of elasticity, or obvious lathe knife lines on the journal surface are found, replacement treatment should be given.
6, see forklift accessories to protect the surface is intact.
Most accessories are coated with protective coating when they leave factory. For example, piston pins and bearing bushes are usually protected by paraffin while piston rings and cylinder liners of forklift trucks are coated with rust-proof oil and wrapped in wrapping paper. The antirust oil such as valves and pistons of forklift engine accessories are sealed with sealed plastic bags. If it is found that the seal sleeve of the sealing bag is damaged, the packing paper is lost, and the rust-proof oil or paraffin is lost, it should be returned or exchanged.
7, see whether there is loosening of adhesive fittings.
When inspecting forklift parts consisting of two or more parts, the parts are formed by pressing, gluing or welding. No loosening is allowed between the parts, such as forklift pump plunger and adjusting arm are combined by pressing; forklift clutch pressure plate and clutch plate are riveted together. Friction plate and steel plate of forklift truck are riveted or bonded; filter core skeleton and filter paper of various filter elements are bonded; circuit and wire head of electrical equipment are welded. If there is any loosening during purchase, it should be exchanged.
8, see whether the rotating parts are flexible.
When choosing turning parts such as oil pump of forklift truck, turning pump shaft by hand should be flexible and without sticking; when choosing needle roller bearing, one hand supports the inner ring of bearing, the other hand rotates the outer ring, and the outer ring should rotate freely and quickly, and then stop gradually. If the rotating parts fail to turn properly, it indicates that the internal rust or the shape has been deformed, which will affect the use, it should be returned or exchanged.
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Next: Forklift accessories knowledge: forklift engine oil selection and use
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This will probably be the smallest outfit post ever on my blog! XD; I only had work two days last week since Monday was a holiday and I took Thursday and Friday off to go to my hometown! ^w^ Since I took pictures of my outfits during my trip anyway and I will make a blog post about it later this week, I thought I would only post my work outfits from last week. ~
There's one outfit that is more on the casual side and one that is more OTT! ^w^
~ Outfit from May 22th! ~
Hat; Taobao
Blouse; Dear Celine
Skirt; Wear It Cuter
Socks; Offbrand
Shoes; Bodyline
Bag; Taobao
Accessories; Paradise Rose & Offbrand
I went for some Country Lolita vibes in red and pink on that day. ~ I haven't worn that skirt in so long and I kind of missed wearing lots of red! ^w^ I coordinated it with a long sleeved pink blouse and pink socks with strawberries. :3 I also chose accessories themed around berries and red too! ~ Finally, my straw hat and bag completes the Country look beautifully. :3
~ Outfit from May 23th! ~
Headbow; Oo Jia
Blouse; Anna House
Jumperskirt; Oo Jia
Socks; Secret Shop
Shoes; Bodyline
Bag; Loris
Accessories; Bodyline, Chocomint & Offbrand
I haven't worn that dress in so long! ;o; I really wanted to wear something a bit special on that day since I was feeling a bit down and tired and I wanted to cheer myself up! ^w^ So I coordinated my pink Milky Planet jumperskirt with a light pink blouse and socks. ~ I also chose pearly and tulle accessories for a elegant touch and they are themed around stars and ponies to match the print. :3 I really liked that outfit! ~
So that was my two work outfits from last week. ^w^ As I said I will do another blog post with my adventures in my hometown later this week which will include my outfits from the weekend! ~
Until next time my fluffy cuddly kittens! :3


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|About IPO
position referral
RECREATION CENTER DIRECTOR — Oak Park Recreation Department, Salary: $9,700-$12,700 and benefits. Will consider related field and/or experiences. Starting date August 1st. Contact Chuck Zept, Supt. of Recreation Centers, Village of Oak Park Recreation Department, 1 Village Plaza, Oak Park, Illinois 60302 (312) 383-6400, Ext. 210.
BUSINESS MANAGER—Park District of Highland Park, Salary: $15,000. Required: B.S. degree in Finance, Accounting or related field. Minimum three years' experience, preferably in Municipal Finance; two years experience with MBA. Knowledge of accounting, budgeting, internal audit, systems and control procedures. Contact Earle Hodgen, Assistant to the Director, Park District of Highland Park, 1801 Sunset Road, Highland Park, Illinois 60035.
SUPERINTENDENT OF GOLF ACTIVITIES—Park District of Highland Park, Salary: Open. Under direction of Director of Parks and Recreation, plans and organizes maintenance and improvements to golf course and related golf facilities. Demonstrated interest and experience in golf. Contact E. W. Nance, Director, Park District of Highland Park, 1801 Sunset Road, Highland Park, Illinois 60035. Closing date: August 6, 1976.
GOLF COURSE MANAGER/ ICE RINK ASSISTANT MANAGER—Salary $9,500 to $11,500. Requirements: Degree and experience preferred. Knowledge of golf and ice skating mandatory, plus management ability. Send resume to William E. Hughes, Director Parks and Recreation, Niles Park District, 7877 Milwaukee Avenue, Niles, Illinois 60648.
Illinois Parks and Recreation 19 July/August, 1976