text:
- Methodology article
- Open Access
pMAA-Red: a new pPZP-derived vector for fast visual screening of transgenic Arabidopsis plants at the seed stage
- Muhammad Amjad Ali†1,
- Kausar Hussain Shah†1 and
- Holger Bohlmann1, 2Email author
© Ali et al.; licensee BioMed Central Ltd. 2012
- Received: 16 March 2012
- Accepted: 1 June 2012
- Published: 2 July 2012
The production of transgenic plants, either for the overproduction of the protein of interest, for promoter: reporter lines, or for the downregulation of genes is an important prerequisite in modern plant research but is also very time-consuming.
We have produced additions to the pPZP family of vectors. Vector pPZP500 (derived from pPZP200) is devoid of NotI sites and vector pPZP600 (derived from pPZP500) contains a bacterial kanamycin resistance gene. Vector pMAA-Red contains a Pdf2.1: DsRed marker and a CaMV:: GUS cassette within the T-DNA and is useful for the production of promoter: GUS lines and overexpression lines. The Pdf2.1 promoter is expressed in seeds and syncytia induced by the beet cyst nematode Heterodera schachti in Arabidopsis roots. Transgenic seeds show red fluorescence which can be used for selection and the fluorescence level is indicative of the expression level of the transgene. The advantage is that plants can be grown on soil and that expression of the marker can be directly screened at the seed stage which saves time and resources. Due to the expression of the Pdf2.1: DsRed marker in syncytia, the vector is especially useful for the expression of a gene of interest in syncytia.
The vector pMAA-Red allows for fast and easy production of transgenic Arabidopsis plants with a strong expression level of the gene of interest.
- Transient expression
- pPZP family vectors
- Marker gene
- Agroinfiltration
- DsRed
- Agrobacterium
- Arabidopsis transformation
Modern plant research relies heavily on the use of transient and stable transformation with the help of Agrobacterium tumefaciens[1, 2]. This bacterium is a natural genetic engineer and transfers a part of its large Ti plasmid into host plants to induce cell division and the synthesis of opines. For use in genetic engineering, the Ti plasmid has been divided into a helper plasmid which is devoid of the T-DNA and remains within the Agrobacteria and a binary vector carrying the T-DNA which can be manipulated in E. coli. The first widely used binary vector was pBIN19 [3]. Many derivatives have been described and some are still in use today although other binary vectors which are smaller, have a higher copy number, and different selectable markers for use in bacteria (E. coli and Agrobacteria) and in plants have been introduced (for review see [4, 5]). One popular series are the pPZP vectors [6] which were also the basis for the pCAMBIA vectors [7]. We have recently published an improved pPZP vector (pPZP3425) which was equipped with a kanamycin resistance gene for selection in Agrobacterium [8] . The strong 35 S CaMV promoter driving the plant resistance gene for kanamycin resistance was replaced by the weaker nos promoter because it had been shown that the 35 S promoter driving the plant resistance marker in the original pPZP vectors can lead to ectopic expression of the transgene [9, 10]. Furthermore, pPZP3425 contains an expression cassette which consists of an intron-containing GUS gene driven by a strong constitutive promoter (35 S promoter with doubled enhancer plus omega element as translational enhancer). This vector has successfully been used in our laboratory.
Plant selectable markers for the pPZP vectors include kanamycin and gentamycin. Both markers work well for a variety of plant species. Kanamycin is perhaps the most widely used selectable marker for plant transformation. Kanamycin and gentamycin as well as other antibiotic markers have the disadvantage that they are usually used under sterile conditions. In case of Arabidopsis this means that to isolate transgenic plants the seeds have to be sterilized and grown on a sterile agar medium containing the antibiotics. Recently it has been shown that the selection of transgenic plant lines containing a kanamycin marker gene can be done by culturing the seedlings on rockwool saturated with MS medium without sugar but containing the selective agent [11]. Since the medium does not contain sugar, sterile conditions are not necessary, saving costs and labour. However, extreme care has to be taken that the seedlings do not run dry. Other markers that also circumvent the need to work under sterile conditions use resistance against herbicides, especially phosphinotricin (BASTA). The herbicide can be sprayed onto plants growing in soil to select for those containing the Bar gene which mediates resistance against phosphinotricin [12]. Fluorescent proteins have also been reported as markers for plant transformation including Arabidopsis [13–17]. For Arabidopsis transformation, DsRed, GFP, and GFP variants have been used as markers driven by seed-specific promoters derived from other plant species [18, 19].
During cloning of a vector for transient expression we realized that the pPZP vectors contain 3 NotI sites in their backbone such that this eight-cutter could not be used in the polylinker. Starting with pPZP200, we have therefore removed all NotI sites from the vector backbone as well as other unnecessary parts to produce pPZP500. By replacing the spectinomycin resistance gene with the nptII gene we also produced the vector pPZP600.
The vector pPZP500 does not contain a plant selectable marker as this is not needed for transient expression. However, since pPZP500 was much smaller than the original pPZP vectors, it could be the basis of a new binary vector (pMAA-Red) for stable transformation of Arabidopsis. For that we included a DsRed gene driven by the Pdf2.1 promoter and the GUS cassette from pPZP3425. The Pdf2.1 promoter was chosen because it is strongly expressed in seeds and in syncytia, feeding sites induced by the beet cyst nematode H. schachtii in Arabidopsis roots [20, 21]. In addition, we replaced the spectinomycin resistance gene used for selection in Agrobacteria by a kanamycin resistance gene.
Construction of pPZP500 and pPZP600
Vector pPZP500 contains the streptomycin/spectinomycin resistance gene (aadA, encoding aminoglycoside-3"-adenyltransferase) which can be used in E. coli and in Agrobacteria. However, in our hands spectinomycin selection was not as tight as kanamycin selection. We, therefore, replaced the spectinomycin resistance gene with the nptII gene which yielded the vector pPZP600 (Additional file 2: Figure S2) as described in Methods.
Construction of pMAA-Red
List of oligonucleotides used in this work
Production of transgenic Arabidopsis lines using pMAA-Red
A large variety of binary vectors for plant transformation have been described. Among them the pPZP series of vectors [6] and the derived pCAMBIA vectors [7] are especially popular. A prominent feature of these vectors is their stability in bacteria, the high copy number, and the relatively small size. One disadvantage was the use of the CaMV promoter for the plant selectable marker and the use of spectinomycin and chloramphenicol as selectable markers for bacteria which led us to construct the vector pPZP3425 [8]. In this vector the CaMV35S promoter for the plant selectable marker (kanamycin) was replaced by the weaker nos promoter and a kanamycin resistance gene for selection in bacteria was included in the vector backbone.
Although pPZP3425 proved useful for our purposes in producing promoter:: GUS lines (by replacing the CaMV promoter in the GUS cassette with a promoter of interest) or overexpression lines (by replacing GUS in the GUS cassette with a gene of interest) [8], selection of homozygous transgenic lines with a strong expression of the gene of interest was still a lengthy procedure. Since we were interested to produce a large number of transgenic Arabidopsis overexpression lines with a strong expression level for putative antimicrobial peptides we set out to construct a pPZP vector that would have three important features: First, it should allow us to use selection or screening on soil to avoid growing Arabidopsis under sterile conditions. Second, we wanted to easily select lines with a strong expression level. Third, the vector should be used for the expression in syncytia using specific promoters that would not be active in seedlings or leaves.
The first precondition excluded the use of antibiotic resistance such as kanamycin or hygromycin or the use of metabolite resistance genes such as the E. coli-derived phosphomannose isomerase which allows growth on mannose [25] or the Streptomyces rubiginosus xylose isomerase (xylA) gene which allows growth on xylose [26] as a plant selectable marker for which sterile conditions would have to be used. Herbicide resistance would avoid the need for sterile growth but all T1 seedlings have to be grown for selection. In contrast, if using a fluorescent marker that is expressed in seeds, only the transgenic seeds obtained after transformation would have to be grown further and no additional treatment would be needed. Such an approach is especially important if a large number of transgenic lines have to be produced. An example is the work of [27] who conducted a high-throughput screen in Arabidopsis for castor genes that would lead to changes in hydroxy fatty acid composition in seeds. We decided to try the DsRed gene as a screenable marker [13–17]. This leaves the possibility to use in addition GFP or other most frequently used fluorescent markers as reporter genes. Furthermore, DsRed has a rather weak fluorescence which might seem to be a disadvantage. However, this weak fluorescence makes it easier to identify seeds with a different level of fluorescence.
Expression of the gene of interest varies largely in independent transgenic lines [24] due mainly to position effects. One way of reducing this variation is the inclusion of matrix attachment regions in the T-DNA. However, this only worked with gene silencing mutants, which limits the use of these vectors [28]. We have demonstrated here that the use of the vector pMAA-Red allows an easy and efficient selection of transgenic lines with a strong expression of the gene of interest in wild-type Arabidopsis plants. Of course, mutants could also be used, if needed. Pre-selection of lines with a strong DsRed expression in seeds according to their fluorescence reduces the number of lines that have to be tested at the transcript or protein level.
Our third precondition was that the vector should allow the use of syncytium-specific promoters instead of the CaMV promoter which is active in most tissues of Arabidopsis plants. We have recently shown that several genes are expressed in syncytia which are normally expressed in pollen [23], such as MIOX4 and MIOX5, or in seeds, such as Pdf2.1 whose promoter is used here to drive the expression of DsRed in seeds. Using the MIOX4 or MIOX5 promoter would allow a specific expression in syncytia which could be useful for genes with a negative effect on plant growth. However, screening such transgenic lines would require the analysis of expression of the gene of interest in syncytia. Such a screening is very time consuming because syncytia have to be cut out from infected roots. In this case a pre-screening that would reduce the number of lines would lead to a significant reduction of time and effort. Thus, the pMAA-Red vector that we have constructed is especially useful for the syncytium-specific expression of transgenes or for similar cases where the expression of the transgene would be restricted to tissues that could not be easily screened. Promoter::reporter constructs and overexpression lines can be produced from this vector as from pPZP3425 [8]. The CaMV promoter can be replaced by a promoter of interest using NcoI and one of the unique sites in the polylinker. The GUS sequence can be replaced with a sequence for overexpression of a gene of interest by using NcoI and BamHI. If the sequence to be cloned contains NcoI or BamHI site, it is usually possible to use a restriction enzyme that produces compatible cohesive ends with NcoI and BamHI. For instance, BspHI, PciI, and FatI produce cohesive ends that are compatible with NcoI.
After transformation of Arabidopsis plants it takes about 3–4 weeks until T1 seeds can be harvested and inspected for fluorescent seeds. A big advantage of transformation with the vector pMAA-Red is that only these selected seeds have to be grown on soil to produce the T2 generation. After another 4 weeks, the first siliques of these plants can be screened for a 3:1 segregation of fluorescent seeds which can then be used to produce homozygous T3 seeds for further analysis. Again, the first siliques of these plants can be used to select homozygous lines and only those will be grown for maturity, which will take a total of 6 to 8 weeks. Depending on the growth conditions, the whole procedure from transformation to harvesting homozygous seeds could be completed within four month.
We have constructed compact pPZP vectors without NotI sites having either bacterial spectinomycin or kanamycin resistance (pPZP500 and pPZP600, respectively) and a vector (pMAA-Red) which allows an easy production of transgenic Arabidopsis overexpression lines with strong expression levels of the gene of interest.
Cloning methods
Standard procedures were used for restriction enzyme mediated DNA digestion, ligation, and transformation [29]. Restriction enzymes and T4 DNA ligase were from Fermentas -Thermo Fisher Scientific and New England Biolabs. The E coli strain DH10B was used throughout this work. PCR was done using Eppendorf Mastercycler Gradient.
Construction of pPZP500
Vectors used in this study
pPZP200 (Additional file 1: Figure S1)
Spectinomycin resistance gene for bacterial slection
CaMV 35 S 2X promoter with omega element, LacZ operon, GUS, CaMV terminator (GUS cassette), kanamycin resistance gene for bacterial and plant selection
pPZP500 (Figure 1)
pPZP200 modified backbone without NotI sites
This study
pPZP5025 (Additional file 2: Figure S2)
pPZP500 with GUS cassette
This study
pPZP3425 containing pPDF2.1::DsRed instead of the 35 S::GUS cassette
Bohlmann, unpublished
pPZP600 (Additional file 2: Figure S2)
pPZP500 with kanamycin resistance gene for bacterial selection
This study
pPZP650 (Additional file 2: Figure S2)
pPZP600 with PDF2.1 promoter, DsRed gene, and 35 S terminator
This study
pPZP653 (Additional file 2: Figure S2)
pPZP650 with new polylinker
This study
pPZP6535 (Additional file 2: Figure S2)
pPZP653 with GUS cassette
This study
pMAA-Red (Figure 3)
pPZP6535 with PDF2.1 promoter, DsRed gene, nos terminator and GUS cassette
This study
Construction of pPZP600
The spectinomycin resistance gene of pPZP500 was replaced by the nptII gene. The nptII gene (~1 kb) was amplified from pPZP3425 using KanforMph and KanrevMph primers. The pPZP500 vector backbone was amplified by using PZP500Mphfor and PZP500Mphrev primers. Both primers had a Mph1103I site at their ends. Insert and vector fragment were digested with Mph1103I, ligated, and transformed into E. coli to yield pPZP600 (Additional file 2: Figure S2).
Plant growth
N. benthamiana and Arabidopsis plants were grown in a growth chamber at 25 ± 1°C, with a 16 hour light/8 hour dark photoperiod and approximately 65% humidity.
Agrobacterium transformation
Binary plasmids were transformed into A. tumifaciens strain GV3101 by the freeze-thaw method [30]. Agrobacteria were selected on YEB plates with appropriate antibiotics which included 25 μg/ml gentamycin and 35 μg/ml rifampicin for Agrobacteria. Selection for binary plasmids was done with 200 μg/ml spectinomycin (for pPZP5025) or 50 μg/ml kanamycin (for pMAA-Red).
Agroinfiltration of N. Benthamiana
Agrobacteria were grown to an OD600 of 0.8 overnight in an incubator/shaker at 28°C. Bacteria were harvested by centrifugation at 5000 rpm for 6 min in a table top centrifuge at room temperature and suspended in infiltration medium (10 mM MES pH 5.6, 10 mM MgCl2 and 100 μM acetosyringone) to obtain bacterial suspensions of the an OD600 of 1.0. After incubation for 2 hr at room temperature, Agrobacterium suspensions were infiltrated in the abaxial side of leaves by using a 1 ml syringe without a needle. Infiltrated plants were kept under the same growth conditions as mentioned above. For co-infiltration of the RNA silencing inhibitor P19, an equal volume of a bacterial suspension harbouring pBin61P19 [31] was added prior to infiltration.
Arabidopsis transformation
The vector construct pMAA-Red was transformed into Arabidopsis ecotype Col by the floral dip method [32]. Seeds of transformed plants were harvested and photographed under an inverse microscope equipped with a DsRed fluorescence filter (Axiovert 200 M; Zeiss, Hallerbergmoos, Germany) and an integrated camera (AxioCam MRc5; Zeiss). Fluorescent seeds (T1) were selected and put on soil to grow the next generation (T2), of which the first mature silique was detached from the plant and examined under the microscope for fluorescent seeds to check for 3:1 segregation. These seeds were then used to produce homozygous lines. Seeds that were kept in the dark at 4°C still showed fluorescence after several years.
GUS staining
GUS staining of the N. benthamiana leaves was done by overnight incubation in X-Gluc solution (50 mM sodium phosphate buffer pH 7.0, 10 mM EDTA pH 8.0, 0.1% (v/v) Triton X-100 and 0.5 mg/ml X-Gluc) at 37°C [33] followed by several washings with 70% ethanol to remove the chlorophyll from leaf tissues.
GUS activity assay
GUS activity was measured according to Jefferson et al. [33] with some modifications in black 96-well Greiner plates. To 100 μl of total protein extract 50 μl of 4 mM 4-MUG was added. The reaction was incubated at 37°C for 5 min and then 50 μl of 0.5 M Na2CO3 was added to stop the reaction. Fluorescence was measured at 355 nm excitation and 460 nm emission in a FLUOstar Omega micro plate reader (BMG Labtech) using 4-MU standards (10 mM stock in ethanol and diluted in GUS extraction buffer) in the range of 1–100 μM.
Nematode infection and examination of fluorescence in syncytia
The seeds of a pMAA-Red transgenic Arabidopsis line were surface-sterilized for 7 min in 10% Chlorox (v/v), submerged for 5 min in 70% (v/v) ethanol, and then washed three times in sterile water. The sterilized seeds were placed on a modified 0.2 concentrated Knop agar medium supplemented with 2% sucrose [34]. H. schachtii was multiplied in vitro on mustard (Sinapsis alba cv. Albatros) roots growing on 0.2 concentrated Knop medium supplemented with 2% sucrose [34]. Hatching of J2 larvae was stimulated by soaking the cysts in sterile 3 mM ZnCl2. The juveniles were washed four times in sterile water and resuspended in 0.5% (w/v) Gelrite for inoculation. Roots of 12-day-old Arabidopsis plants were inoculated with about 40–50 juveniles under sterile conditions. At 5 and 10 dpi the fluorescent syncytia were examined using an inverse microscope.
Resistance tests and size measurement of syncytia and nematodes
H. schachtii (Schmidt) infection was done in the same way as described above. Roots of 12-day old Arabidopsis plants were inoculated under axenic conditions with about 50 juveniles per plant. The number of male and female nematodes per cm of root length were counted at 14 dpi. The data were analysed using single factor ANOVA (P < 0.05). As the F-statistic was greater than F-critical, a Least Significance Test (LSD) was applied. At 14 dpi, pictures of male syncytia, female syncytia and females nematodes (30 photos for each) were taken. Syncytia and nematodes were measured using an inverse microscope (Axiovert 200 M; Zeiss, Hallerbergmoos, Germany). The data were analyzed using single factor ANOVA (P < 0.05).
GenBank accession numbers
pPZP500: JX025644
pPZP600: JX025643
pMAA-Red: JX025642
This research was supported by grants P20471-B11 and P21896-B16 of the Austrian Science Fund (FWF). Muhammad Amjad Ali and Kausar Hussain Shah were supported by Higher Education Commission (HEC) of Pakistan. The authors are thankful to Shahid Siddique, Amjad Abbas, Bachar Almaghrabi, Martina Niese and Stephan Plattner for their support. We thank Dr. David Baulcombe for pBin61P19.
Authors' Affiliations
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Sometimes you read a book that you unapologetically recommend to everyone you know, and you pre-order the sequel on Amazon the first day you can. This was one such book for me.
I even Googled Durham's blog and posted a comment about how much I loved the book, and he actually replied. To me, this is akin to receiving a response from Tolkien himself. I love that we live in an era when you can reach out to writers, singers, actors, etc on Facebook, Twitter, and blogs and sometimes even hear back from them.
The final book of the trilogy will be released this October, and I wanted to do something for it on the blog. I asked David if he'd film a short video for my readers (which he was incredibly kind to take the time to do), and I'm going to give away copies of his first two Acacia books to one of you.
Here's how it'll work: In the comments below, share one or several of your favorite summer reads. Because there's really nothing better than a good book in the summer, is there? You can even share a book or books that you're really looking forward to reading for the first time this summer.
At the end of the week, I'll select one person at random and will send them a copy of the first book in the trilogy. If you read it and love it, I'll send you the second book too. If you're not a fan, I'll send you any other book (within reason–no rare leatherbound books from Ron Burgundy's library, please) of your choice.
Thanks for participating, and I really look forward to hearing your recommendations. Feel free to "Like" this entry on Facebook at the bottom of this entry.


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A Lipid Receptor Sorts Polyomavirus from the Endolysosome to the Endoplasmic Reticulum to Cause Infection
Qian, Mengding
Verhey, Kristen J.
Tsai, Billy
Galloway, Denise
Published Versionhttps://doi.org/10.1371/journal.ppat.1000465
MetadataShow full item record
CitationQian, Mengding, Dawen Cai, Kristen J. Verhey, and Billy Tsai. 2009. A lipid receptor sorts polyomavirus from the endolysosome to the endoplasmic reticulum to cause infection. PLoS Pathogens 5(6):e1000465.
AbstractThe mechanisms by which receptors guide intracellular virus transport are poorly characterized. The murine polyomavirus (Py) binds to the lipid receptor ganglioside GD1a and traffics to the endoplasmic reticulum (ER) where it enters the cytosol and then the nucleus to initiate infection. How Py reaches the ER is unclear. We show that Py is transported initially to the endolysosome where the low pH imparts a conformational change that enhances its subsequent ER-to-cytosol membrane penetration. GD1a stimulates not viral binding or entry, but rather sorting of Py from late endosomes and/or lysosomes to the ER, suggesting that GD1a binding is responsible for ER targeting. Consistent with this, an artificial particle coated with a GD1a antibody is transported to the ER. Our results provide a rationale for transport of Py through the endolysosome, demonstrate a novel endolysosome-to-ER transport pathway that is regulated by a lipid, and implicate ganglioside binding as a general ER targeting mechanism.
Other Sourceshttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685006/pdf/
Terms of UseThis article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4515106
- FAS Scholarly Articles [14564]
Contact administrator regarding this item (to report mistakes or request changes)


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Its global sales surged by 1.1% from the same month last year and 23% from the previous month becuase of sales of Tivoli and Tivoli Air, which has led the company's domestic sales growth along with Tivoli since its launch in early March.
Furthermore, the combined contracts for Tivoli brand models -Tivoli and Tivoli Air- exceeded 8,500 in March. Based on the sales uptrend, Tivoli brand will continue momentum of Ssangyong's sales.
Ssangyong's domestic sales recorded 9,069 units, up 29.9% from the previous month and 17.5% from the same month last year helped by robust sales of Tivoli brand models that jumped by 69.7% compared to March 2015 and its other main SUVs.
Ssangyong's exports in March soared by 9.7% over the previous month thanks to Tivoli Air exports to the European markets, while its year-on-year exports dropped by 23.5% due to reduced demands in the emerging markets.
Since the exports of Tivoli brand models increased by 37.1% compared to the same month last year, the company expects great improvement in overseas market soon, especially once Tivoli Air starts to be exported.
Ssangyong introduced Tivoli Air for the first time at Geneva Motor Show in March, and has been carrying out aggressive marketing activities before its global sales from May including participation in regional motor shows.
Choi Johng-sik, CEO of Ssangyong Motor, commented, "Both Tivoli and Tivoli Air create a synergistic effect so that their contracts are continuously increasing," adding, "We will make utmost efforts to meet increasing demand for Tivoli brand by operating production system in an efficient way as well as by launching Tivoli Air globally."


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While Apple probably wishes they'd really locked down the trademark rights to the iPad name long ago, the folks at Proview Technology in Shenzhen have a prior claim to the name and they're taking Apple to court over it. They're asking Apple stop selling the iPad in China (which only went on sale in the area recently), and demand an apology. Proview Shenzhen had registered the trademark in 2001, but their sister company, Proview Taiwan, had sold the rights to the iPad name to a UK firm, even though the Taiwanese company didn't have rights to the trademark. Despite that, a UK company then sold the rights to Apple, which now counts iPad on their official trademark list. Apple had known about this whole mess, and had sued Proview Shenzhen last year to assert their claim to "iPad", but had lost the suit. Now it looks like Proview is taking the fight to Apple.
Apple still doesn't own ipad.com, and with this trademark issue lingering, there's a clear gap in their IP rights. It's not like they don't have enough money to throw at the problem, and that said, odds are slim that this trademark suit will pose any serious roadblock to the advance of iPad in China. Proview makes outdoor LED lighting, so it's not like there's any real competitive overlap here; they probably just want a big fat payoff, and it's hard to blame them. We'll be keeping an eye on the lawsuit's progress following the initial hearing this month.
Source: China Daily


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The PERFECT SUGAR COOKIE
1-1/4 cups sugar……………………………... 2 eggs
1 cup Butter flavor CRISCO (MUST use CRISCO ~ That's what makes them softer and chewier than cookies with butter)
1/4 cup light corn syrup …………………… 1 TB pure vanilla extract
3 cups flour………………………………..…..3/4 tsp baking powder
1/2 tsp baking soda ………………………....1/2 tsp salt
Frosting ~ decorations as desired
Preheat oven to 375. Beat sugar and Crisco in electric mixer at medium until well blended. Add eggs, corn syrup and vanilla ~ beat well. Combine 3 cups flour, baking powder, baking soda & salt ~ add gradually to creamed mixture at low speed. Mix until well blended.
Divide dough into quarters. (If dough seems too sticky/soft to roll, wrap each quarter in plastic wrap & refrigerate about 1 hour.) Spread a little flour on large sheet of waxed paper. Put one of the quarters of dough on floured paper; cover with another sheet of waxed paper. Roll dough to 1/4" thickness. Cut with floured cookie cutter.
Put on ungreased cookie sheet (I still like parchment paper) 2" apart. Sprinkle with sprinkles or wait to frost when they're cooled later. Bake at 375 for 5-9 minutes (depending on size of cookie shape). Do not overbake!! (should just be turning light golden on bottoms) Cool 2 minutes on baking sheet; remove to counter to cool completely. Frost as desired. Makes about 3-4 dozen cookies
I use tubs of storebought frosting. My grandkids like to frost & sprinkle them.


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LEOMINSTER -- A candlelight vigil at city center Thursday evening was marked by the sounds of prayer and song, as faith leaders and advocates honored survivors of abuse perpetrated by intimate partners during national Domestic Violence Awareness Month.
As the sun went down, the Leominster Police Department's domestic-violence advocate, Lesly Borges, stood under a statue at Monument Square Park. She commended work done by the local YWCA, the advocacy organization that supplies emergency shelter, counseling and support for victims of domestic violence.
Despite the organization's tireless efforts, domestic violence remains a lethal force in the community, Borges said.
"There are many people who are not here with us right now because they have died because of domestic violence in their homes," she said.
Half of women who are killed in the United States are killed in the course of intimate-partner violence, according to a recent report released by the Centers for Disease Control and Prevention.
Votive candles ensconced in white paper bags flickered on the ground where about two-dozen people stood for the vigil, bowing their heads as Jazmin Ruiz, daughter of Spanish American Center Domestic Violence Advocate Wanda Ruiz, sang Amazing Grace.
The Rev. Dennis Bradley, pastor of New Life Spanish Christian Church, called on divine forces for protection, then Ward 2 City Councilor Pauline Cormier read a proclamation on behalf of the mayor.
October, the proclamation states, is now officially designated Domestic Violence Awareness Month in Leominster.
Cormier, who is also criminal defense attorney, said earlier in the evening she councils those accused of assaulting a partner, urging them to break the cycle of violence.
Many perpetrators, she said, grew up in a household where abuse was present, but rarely discussed. Now, society has begun moving away from silencing victims.
"This is no longer an issue that's hiding in the shadows, this is not a family's 'dirty little secret' anymore, and I think that has been a huge step," she said.
The Rev. Jim Hinds, pastor of First Baptist Church of Gardner, who also led the vigil in prayer, agreed, citing the scores of women posting #MeToo on social media as evidence.
"More and more people are willing to say, I have been hurt," said Hinds. "Too often people carry it as an inner wound."
For Crystal Guyette, an advocate against abuse of people with disabilities, solidarity is key. She said she stands alongside survivors.
"I want them to know, abuse is not your fault," she said.
Those experiencing domestic violence are urged to call the YWCA 24 hours a day at 1-508-755-9030. Help is also available online at ywcahelp.com.


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By Dr. Mercola
Junk food and sugary beverages contribute to skyrocketing rates of diabetes, high blood pressure, and even strokes — and not just among adults1.
Food and beverage companies spend around $2 billion a year promoting unhealthy foods to kids2, and while ultimately it's the parents' responsibility to provide their children healthy foods and drinks, celebrity endorsements can make this much more difficult than it should be.
The latest example is that of Beyonce, who just signed a $50 million deal with Pepsi as its global brand ambassador.
Another far more bizarre example of unhealthy celebrity endorsements is that of the Olsen twins, who recently released a very limited edition collection of handbags, retailing for an absurd $55,000 per bag. Besides the outrageous price tag, the most shocking thing about these bags is that they're decorated with prescription pills, which has raised more than a few eyebrows.
As reported by Toofab.com3:
"Making the artistic decision even more odd is the knowledge that Mary-Kate Olsen's friend, Heath Ledger, died of an accidental overdose of prescription drugs including pain killers, sleeping pills and anti-anxiety medication."
Giving Prescription Drugs a Glam Makeover
It's hard to tell what the underlying message of the pill-encrusted bags is intended to be, but it certainly comes across as an attempt to glamorize the use of prescription drugs — especially among those who can actually afford both the drugs and the bags.
While I've not heard of any links between the Olsen twins and Big Pharma, these kinds of fashion statements may be among the most effective forms of advertising of all, especially among the younger crowd...
As described in the 1928 book "Propaganda" by Edward Bernays, the father of PR, the public relations business is less about selling things than about creating the conditions for things to sell themselves. And what better way to increase social acceptance of drugs — and hence their use — than having them featured on exclusive fashion items? I bet CEO's at pharmaceutical companies everywhere are smiling from ear to ear at the thought of receiving such fantastic advertising for their wares... It's the kind of marketing that is truly priceless.
The Burdens of a Role Model
Beyonce's new Pepsi deal has also garnered a lot of criticism, which can be seen as a sign that the tide is finally starting to change. It would seem people are getting sick and tired of role models who don't, well, act like role models. Reporting on the singer's controversial decision to attach her face and talent to Pepsi, Frugivoremag.com writes4:
"Reactions have been mixed. Fans view the campaign as a momentous accomplishment for the singer, while others chide Beyoncé for supporting a sugary-soda brand which is a health affront to many American consumers. They even accuse the diva of hypocrisy for appearing in Michelle Obama's 'Let's Move' initiative to encourage good health among children and later, shelling Pepsi cans.
One commenter said: 'Parkinson's, heart disease, obesity, stroke and Alzheimer's tincture. Another celebrity getting paid to keep America on their mainline like heroine. I respectfully decline supporting this travesty.' Another writes: 'With diabetes and other ailments at an all time high, one would wonder why celebrities who claim to care so much about their fans would endorse soda. Almighty dollar.'"
Beyonce has defended her decision stating that the Pepsi brand "embraces creativity and understands that artists evolve" and that "as a businesswoman," this allows her to "work with a lifestyle brand with no compromise and without sacrificing my creativity." Be that as it may, no company on earth pays $50 million for a "brand ambassador" unless they're quite certain their chosen endorser will have a positive effect on their bottom line. In this case, that means more people chugging more Pepsi. Some may argue that celebrities should not be held responsible for the bad choices their fans make, but if it's a bad choice, why attach your name and reputation to it?
Big Pharma has become one of the most powerful influences in the US, if not the world, because of effective marketing. Ditto for the junk food and soda industries. In short, advertising works, and with the right celebrity endorsement, you could sell ice to an Eskimo... The Center for Science in the Public Interest is now urging Beyonce to reconsider her collaboration with Pepsi. In a letter to the star, CSPI writes5:
"More than any other category of food or beverage, sugary drinks are associated with increased risk of weight gain and obesity, which increase the risk of diabetes, hypertension, and heart disease... In fact, each additional sugary drink consumed per day increases the likelihood of a child becoming obese by 60 percent. Each soda consumed per day increases the risk of heart disease in men by 19 percent. Drinking one or two sugary drinks per day increases one's risk for type 2 diabetes by 25 percent...
You occupy a unique position in the cultural life of this country and are an inspiring role model for millions of young people. Your image is one of success, health, talent, fitness and glamour. But by lending your name and image to PepsiCo, you are associating those positive attributes with a product that is quite literally sickening Americans."
Again, while artists want to be respected for their creative and artistic visions, it sure would be nice if more of them would embrace the responsibilities (unwanted as they may be) that come with being a high-profile personality and role model, especially if they have a younger audience, like Beyonce and the Olsen twins do...
Is Beyonce Part of Pepsi's Plan to Cash in on Health-Conscious Consumers?
Pepsi has repeatedly tried to weasel their way into the pantries of health-conscious consumers, so in that respect, the choice of Beyonce fits the bill in more ways than one. Remember Pepsi Raw, which was introduced in the UK in 2008? In the US, a similar product was released under the name Pepsi Natural. This was just one of many of the soda company's attempts at cashing in on the rise in popularity of all things natural and healthy. The attempt flopped, and the product was removed from the market just two years after its release.
Last year, Pepsi Next was released. Pepsi Next claims to have 60 percent less sugar without sacrificing taste, but the secret to keeping its sweet taste comes from the use of not only high fructose corn syrup, but also THREE artificial sweeteners: aspartame, acesulfame potassium, and sucralose, all of which are linked to several dozen serious health risks6. It's all part of the company's plan to beef up their share of profits from the "healthy foods" category, with reports noting they hope to boost their nutrition business from $10 billion to $30 billion by 20207.
This is a classic corporate move. Find something that people are interested in and sell it to them even if it is deceptive and worsens their health. Most of these companies have absolutely no interest in protecting or promoting good health; they are focused on their bottom line profits — and star power like Beyonce, and many others, help them do just that.
Beware: It Starts Early — Your Child's Taste Preferences are Created by Age 3!
Did you know that when you feed preschool-aged children junk foods high in sugar, salt and unhealthy fats, it has a lasting impact on their taste preferences? Think about it — how many kids and teens do you know that don't like soda and sweet foods? Part of the reason for this limited taste preference is the fact that their taste buds are literally trained within the very first years of life (in some cases from birth, as infant formula can contain loads of sugar!).
In a recent study8, all of the children tested showed preferences for junk foods, and all (even those who were just 3 years old!) were able to recognize some soda, fast food, and junk food brands. The researchers concluded what you probably already suspect: kids who were exposed to junk food, soda and fast food, via advertising (and also because their parents fed them these foods), learned to recognize and prefer these foods over healthier choices.
This does have an impact on their health, as nutrients from quality foods are critical in helping your child reach his or her fullest potential.
Another study9 from British researchers revealed that kids who ate a predominantly processed food diet at age 3 had lower IQ scores at age 8.5. For each measured increase in processed foods, participants had a 1.67-point decrease in IQ. As you might suspect, the opposite also held true, with those eating healthier diets experiencing higher IQ levels. For each measured increase in dietary score, which meant the child was eating more fruits and vegetables for instance, there was a 1.2-point increase in IQ.
The reality is, the best time to shape your kids' dietary habits is while they're still young. This means starting from birth with breast milk and then transitioning to solid foods that have valuable nutrients, like egg yolk, avocado and sweet potatoes. (You can easily cross any form of grain-based infant cereal off of this list.)
From there, ideally you will feed your child healthy foods that your family is also eating -- grass-fed meats, organic veggies, vegetable juice, raw dairy and nuts, and so on. These are the foods your child will thrive on, and it's important they learn what real, healthy food is right from the get-go. This way, when they become tweens and teenagers, they may eat junk food here and there at a friend's house, but they will return to real food as the foundation of their diet -- and that habit will continue on with them for a lifetime.
My newly revised nutrition plan offers a step-by-step guide to feed your family right, and I encourage you to read through it now. You need to first educate yourself about proper nutrition and the dangers of junk food and processed foods in order to change the food culture of your entire family. To give your child the best start at life, and help instill healthy habits that will last a lifetime, you must lead by example. Children will simply not know which foods are healthy unless you, as a parent, teach it to them first.
Straight to the Source: Dr. Mercola at mercola.com.


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A glimmering prize for the desolate,
Jewelled with stars that pave heaven at her feet,
With breasts full with the promise of release,
And tender, sensual fingers that suffocate.
Wrapped with precious furs in dusk's red light,
Youthful fires burnish her pale opal eyes,
Rings on her fingers, pearls around her thighs,
She beckons you like a perfumed flame in eternal night.
She reclines on her resplendent bed,
Scattered with flowers upon her gilded stage.
An oiled, scented vision of blistering love and rage
Lit by golden pools of light above her head.
Like Olympia with an orchid behind her ear,
Like a timeless bruise that never heals, again
This glittering whore of desperate men
Draws you like a jaundiced candle nearer.
In response to daily prompt: Ostentatious / discover challenge In the Style of
Image: Wasserschlangen I by Gustav Klimt
This was my attempt at writing a poem about hope in the style of 19th century French Symbolist poet Charles Baudelaire. Olympia was a painting of a prostitute by Édouard Manet.


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Home | Cart Review | Site Map | Tracking
Holiday's & Observances
4" Rectangle Photo/Logo Brownies (2 doz. min.)
Individually wrapped in cello bags with ribbon.
Image size is 4", brownies bake to approximately 4"
6 ounces of moist, fudgy delicious brownie!
Ordered by the dozen, one image per dozen brownies.
Brownies should be ordered at least 2 weeks prior to the occasion.
We use a special printer that uses food coloring. The images are printed on frosting sheets. We use frosting sheets, not rice paper, because they provide the highest quality image and have no flavor or taste. There is also no drying time needed so orders can be produced very quickly. You can mail us an original B&W or color photo (wallet size to 8" x10"). Or mail us any picture or business card. Copies of photographs can be used if they are done professionally (i.e. Kodak Picturemaker). We can scan it into our computer and make any necessary changes. All originals will be returned with your order. You can email us the image file in JPG, BMP, TIF, GIF, PDF, EPS, or PSD format. Images should be at least 200 dpi and 100% size.
Not sure what image you want? Let us customize an image for you. There is no additional charge for this service!!
Custom Brownies ranging from Personalized Brownies to Photo Brownies to Logo Brownies for any special event. We can design any brownie that looks great and taste delicious!
Please email or call us for your order. Discount pricing is available for quantity orders.
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Corporate DISCOUNTS available for multiple baskets. Please call or email for pricing.
Click Add to Cart to order this product.
Email us at firstname.lastname@example.com with any questions about this or any of our other products.
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Shop Online 24 hours a day 7 days a week. 100% Secure Shopping
APO & FPO We only ship items from our Military Page to these addresses.
Corporate DISCOUNTS available for multiple gift baskets. Please email: email@example.com or call: 866-732-8130 for pricing.
We deliver to addresses within the United States:
Alabama AL . Alaska AK . Arizona AZ . Arkansas AR . California CA . Colorado CO . Connecticut CT . Delaware DE . Florida FL . Georgia GA . Hawaii HI . Idaho ID . Illinois IL . Indiana IN . Iowa IA . Kansas KS . Kentucky KY . Louisiana LA . Maine ME . Maryland MD . Massachusetts MA . Michigan MI . Minnesota MN . Mississippi MS . Missouri MO . Montana MT . Nebraska NE . Nevada NV . New Hampshire NH . New Jersey NJ . New Mexico NM . New York NY . North Carolina NC . North Dakota ND . Ohio OH . Oklahoma OK . Oregon OR . Pennsylvania PA . Rhode Island RI . South Carolina SC . South Dakota SD . Tennessee TN . Texas TX . Utah UT . Vermont VT . Virginia VA . Washington WA . West Virginia WV . Wisconsin WI . Wyoming WY
We also deliver a few selected products to APO / FPO addresses (ground shipping only) see our


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Beautiful wooden iPad 3 or iPad 4 case of 100% real cherry wood. The case is very strong and lightweighted. All functions of the iPad, like the dock connector, volume buttons and the camera are easily accessible, without having to remove the case. Applying of the cover is very easy, because it exists of 2 parts. You don't have to be affraid to damage your iPad, because the inside of the case is protected by soft felt.
You may also be interested in the following product(s)
Bamboo Wooden iPad suitcase de Luxe
Regular Price: €99.95
Special Price: €59.99
iPad 3 or 4 wooden case bamboo
Regular Price: €37.95
Special Price: €19.99


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PHP School Management System
Presupuesto ₹1500-12500 INR
Need an Expert in updating PHP Script. Need to hide features, add features and add bulk sms, payment gateway, bulk emails etc. Script: [login to view URL]
All details in attached file.
34 freelancers están ofertando el promedio de ₹11138 para este trabajo
Hi there, I have gone over the attached sheet and the code canyon link. We are familiar with school management solutions. Infact we have built something similar as visible at: [login to view URL] Más
Hello Sir/ Ma'am, We are interested in your project. Ready to start this project immediately! Currently we are working on User Management, Project Management System and Order Management System School Portal/ERP: Más
Hello Without wasting your time please let me tell you, We already have SMS on which we are working on from last 6 months, that Inculde all the feature you required and if you need we can make chances as well as acc Más
i am accurate and expert website developer and designer, i can integrate SMS, mail and Payments with your codecanyon script. please have a look at my past work reviews and portfolio. thank you Mukesh
Hi there. I am very much interested in updating your script on school management system. I am an experienced website developer focused on creating modern websites using PHP, MySQL, WordPress, HTML5, CSS and JavaScri Más
Dear Client, you are searching for an Expert developer to modify this particular SMS script according to specification in the attached doc. I have Expertise in web based CRM and ERP systems. I can do it. I am a Co Más
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Dear sir, First of all am bidding this project because i have a experience on customizing the same code. I have done that for another client. So i have a good knowledge and know flow of code. I can complete you Más
Available Timings 11.30 AM to 9.00 PM IST Monday to Friday. Technical Skills - 9+ years of experience in web development - 2+ years of experience in using Express and Angular JS - Developed wordpress plugins and Más
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Hi, I have read attached documents and I am very confident that I can get this project done in quick and efficient way.I am a Full Stack Developer with 6+ years experience in Laravel,Symfony2, 3, Meteor, AngularJS, Ex Más
We have already a ready made system of School Management. If you Consider us to full fill your job then we will try our best level. We're looking forward to some feedback from you. Thanks & Regards Más
Hello there, I am a web developer with 10 plus years experience in web development and which you have post the URL of school management system. so that i can provide to you same ready to use SMS. If you are intere Más


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44 results
For its Face range, Jurlique transports botanical properties from seeds to the skin. From Earth to extraction, Jurlique's product process is honest and pure. In fact, 8.000 rose petals sare handpicked and distilled for just one bottle of the brand's bestselling Rosewater Mist spray. Guide to your skin to an ultimate state of balance and comfort with Jurlique skin care, and rest assured in the Australian brand's biodynamic farming methods.


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Sunday, February 10, 2019
Veteran Family Paint Day at the PRAA
February 10: 1:00 pm - 3:00 pm
Veterans and their families are welcome to come and make art at the Paso Robles Art Association.
Free of charge for veterans, active military and their families, all materials are provided thanks to funding from the California Arts Council in connection with the March 2019 Veterans Voices II art exhibition at Studios on the Park.
November 29 - February 17
Thankful is the focus of the Paso Robles Joint Unified School District Gallery's annual themed show featuring artwork by students from all Paso Robles' schools, grades K-12. Please come enjoy how their talented student artists of all ages express what they are thankful for.
January 31 - February 24
Creative Couples ° Partners in Art ° Partners in Life
Couples who share a home and share creative passions navigate their relationship and their art in unique ways. Some duos collaborate and create pieces of art together. Others work in distinct mediums, but use their artistic experience to consult with the other, offering suggestions and support.
This exhibition explores the ways creative partners interact, inspire and enhance each other's creative process. How does one keep a sense of self, yet encourage the other, whether working together or in separate studios? Does having two creative people in the house bring inventive thinking to other areas of their life? Several creative couples will share their unique works of art in many mediums and offer insight into their inspirational interactions.
Heart of the Matter
January 31 - February 27
With their vision and creativity, artists get to the heart of the matter and show us the core, the soul, the essentials of art. Whether it's the quintessence or the nuts and bolts, see Paso Robles Art Association members' view of the heart of the matter in their Showroom gallery.
Black and White
February 01 - February 28
Digital artists Deb Hofstetter and Dean Crawford Jr. offer a carefully curated collection of monochrome images in this special photography showcase. Selected artworks feature beautiful grays, clean whites and deep blacks with striking contrast, shapes and texture. All pieces are matted, framed and ready to take home and hang.
In addition to celebrating their new partner, Deb and Dean have curated a collection of their country home and garden images for their January showcase.
COLORbar: "Purfect Date" & "Wild Flowers" by Jordan Hockett
February 01 - February 28
Experience watercolor painting with Studios on the Park's unique COLORbar. Create your own masterpiece by filling in already prepared original designs by resident artist Jordan Hockett. The $10 participation fee includes all materials (but no instruction) and supports Studios' essential arts services to local children and adults.
Hearts for Art
February 01 - February 28
During the month of February, show your love for a favorite work of art by placing a paper heart on the floor in front of your artwork crush.
Step 1: Pick up a single heart and hold it close until you're ready.
Step 2: Fall in love with a piece of art and make it official by placing your heart ON THE FLOOR in front of a work of art you love.
Step 3: Snap a photo of your heart placed next to your artwork crush, and post it to your favorite social media site, tagged with #studiospr and #heartsforart.
Please note: Not everything at Studios on the Park is in the mood for love. Hearts and photography are permitted in all studios and galleries except where there are No Photography signs. Ask your friendly artist or docent if you are unsure.
Happy heart-ing!


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Perfect for export and import companies, or any organisation with offices, premises, clients or agents in several locations.
There are three main components:
An Intranet, or Customer Relations Management System, accessible by managers and staff of the organisation, wherever they are in the world. There are full document management facilities, all internal and external contacts and addresses can easily be kept up-to-date, along with calendars, sales & project management, notes, stock control, etc. In fact everything an organisation needs in terms of information management.
A global telephone system which integrates with the Intranet/CRM. Any number of extensions can be provided. Calls can be made to, from, and between software-based phones on Windows or Mac computers, IP desk phones, landline phones and mobiles.
We can provide local, national or freephone telephone numbers in almost any country in the world. The easier it is for your clients or agents to contact you, the more business they will do with you.
Wherever you are in the world, we can connect you


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Full time travel is something many of us aspire to, yet at first glance, making this dream a reality seems to be a daunting task. Fear not. Working on the road is as easy as ensuring you have a good wifi connection, a great data plan and dependable cell service. Mobile Internet is the key. We've learned a lot of the trade secrets through trial and error and want to share our newly acquired knowledge with you.
On of the biggest hurdles for full-time travelers is figuring out how in the world they are going to make money to not only travel, but to feed themselves and pay for necessities like clothing and shelter.
If you have a job that enables you to work remotely, you are ahead of the game. But the logistics of how to make travel and work a reality can be daunting. In fact, it's one of the top, if not the top, questions my husband Dan and I are asked.
You see, Dan works as a software engineer while we travel full-time, and it's absolutely critical that he stays connected everywhere we go. Whether it's in the National Forests in VA or the rural towns of CO, he must have access to the Internet.
I dabble in travel writing for various publications and websites, including TravelingMom and Kelloggshow.com. We also are very active on YouTube and publish videos often on our two channels, our family vlog and our kids' channel that features pranks and kayaking.
And our kids are homeschooled, and utilize the Internet for a whole host of research projects, blog uploads for our site as well as their sponsors, etc.
So, we need a mobile office with phone, email and high speed data.
Short of sitting in a coffee shop for eight hours every day, we struggled to find the right combination to make it all work for us, both financially and technically. We did all the research we could think of to find good, cheap data for our cell phones, internet connection and high bandwidth data connections.
We've tried everything, including going uber cheap and let me tell you up front that going cheap is not the answer. Remember the old adage, "You get what you pay for?" It applies exponentially here. Cheap data is bad data.
Here I take the guesswork out of work and travel. Here are the very things we utilize to make work and travel as seamless as possible.
Cellular Service
Cell phone service is available nationwide with pretty good 4G LTE network coverage. This means cell phones should work in all but the most remote areas we visit. There is always the option to make WiFi calls where cell service is not available.
We started out with Verizon and then switched, in an effort to save money, to T-Mobile. We had high hopes. We now "affectionately" call them, "T-Maybe". This was, hands down, the biggest work-related mistake ever.
T-Mobile works in big cities and that's about it. If you don't travel and never leave your urban environment, sure, the savings are well worth the switch. But, if you are like us and travel outside of these areas, you will be loathe to find anything except extended network. Verizon is definitely the most expensive, but it also has the best coverage.
Luckily, we were referred to Ryan Maharg, a Verizon Specialist. He hooked us up with a great deal and we are in the process of switching everything over to Verizon. Ryan has been awesome. I highly recommend contacting Ryan at [email protected], tell him #KelloggShow sent you and he can set you up with cell service, a Jetpack and a customized data plan.
Mobile Hotspot
A mobile hotspot is basically a portable internet connection that connects to the Internet through a cell provider. Personally, I think it's the best invention since disposable diapers. Literally, this mobile internet device can provide an Internet connection to up to 15 WiFi enabled devices, like tablets and laptops.
We use a Novatel MiFi Jetpack 6620L to connect everything we use to the Internet. We can even, in fact, connect our phones to the JetPack when the T-Maybe signal isn't as good.
The only downside is that each device takes some of the bandwidth, which can reduce your connection to a crawl if you don't have a great signal. Also beware of video. Video eats your data so fast which can result in steep overage charges. In fact, we know someone who took his nephew on a road trip and they watched YouTube videos the whole time. Want to know what his bill was? A whopping $1400! Dan would have died!
Cell Signal Booster
A cell phone signal booster does exactly what it sounds like. It boosts the signal from the cell tower, expanding the coverage area … even in dead zones. We went about a year and a half before we got one of these and let me tell you, it's a life saver in places like campgrounds. Campgrounds often claim to have wifi, but the signal is lame. So lame, in fact, that it doesn't reach any but the nearest campsites.
Now we use a Wilson DT4G which uses an external antenna to boost the signal inside our RV. It's provided our cell phones with three bars of 4G LTE where without it we could only get a 2G signal! It also boosts the signal to our mobile hotspot, so we get better Internet connection speeds, which is especially important when multiple devices are connected.
Free WiFi Hotspots
As we all know, there are free wifi hotspots everywhere, all across the country. Coffee shops, laundromats, Sam's Club and most fast food restaurants almost always have free wifi.
But, after trying this a few times, we pegged this as a drag. I mean, who wants to sit in a coffee shop or in the parking lot outside of Sam's Club for up to eight hours a day? And, of course, a purchase at a coffee shop or restaurant is pretty much expected if you want to use their wifi, so it's not really free, yet still cheap — and still a drag.
Many cities and towns also now offer free WiFi in downtown areas or parks, which has proven useful for us on several occasions. You see, we have a mobile hotspot, but it has limited data, 30 gigs on our current plan. And, every now and then we use more than we should and it's then that we utilize the free wifi that some cities and towns offer, if only for supplementing. It's great because I head out and play with the kids at the park and Dan buckles down and cranks out some brilliance!
In addition, many campgrounds, like KOA, offer free WiFi to campers and provide a good quality signal. Some campgrounds have limited coverage areas where the wifi signals don't cover the entire campground, so be sure to ask before you reserve a site. Comcast Cable customers can also take advantage of XFINITY WiFi hotspots, which are popping up everywhere that Comcast offers broadband Internet access.
WiFi Range Extender
I have no idea how this works, but it works beautifully. According to Dan, a WiFi range extender "amplifies the WiFi signal from a WiFi hotspot and makes it easier to connect multiple devices to the WiFi network".
You can use a WiFi range extender to expand the WiFi coverage area, so you can access free WiFi hotspots from within the comfort of your RV. For instance, if you are parked near a restaurant or store or hotel or park that has wifi, you can use the range extender to basically boost the signal to where you are. You know, so that you don't have to actually go inside to use their wifi!
Bet you didn't know that some luxury RVs, like the Newmar King Aire, come fully equipped with a range extender.
Other Considerations
So that takes care of all the work related concerns you may have. But there's one more hurdle and that is the kids. Other than running them ragged and keeping them as exhausted as possible so you can get some much needed work time in, you ought to consider some other road trip ideas to keep them engaged and relatively quiet. I found these to be incredibly creative and my kids love crafts. Of course, we'd be remiss if we didn't mention the beauty of apps and games that make for really great boredom busters … add in a little education and it's a win/win for everyone!
Now get out there, no excuses! See you on the road!


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Welcome back to another exciting week of The Duality and Hypocrisy (NFL) Podcast! We're back this week with a serious journey through Takeland, in which we see which one of the two of us can drop the HOTTTESSSTTT takes Included in that is our discussion of Draft analysis, the game of basketball, NASCAR, and Uday's surge to be Donald Trump's VP.
LOYAL LISTENERS PLEASE!! go to itunes and drop us a review!! really helps us in terms of visibility in the itunes store. Thanks!!
NOTE: Wray's Pick for the Kobalt 400 was Martin Truex Jr
EXTRA SPECIAL COOL NEWS: We are on WUOG 90.5 UGA at 11 am on sundays! check us out on the terrestial!!
See you on the other side.... and just maybe, if you enjoyed it, Follow Uday at @all22_NFL and Wray at @WrayManning or email the podcast at firstname.lastname@example.com! we'll EVEN READ YOUR MAIL ON THE AIR!Check the podcast out on itunes, Tunein Radio, Stitcher, and anywhere else podcasts are found. Check out our twitter at @dnhpodcast !
Tune into the Duality and Hypocrisy (NFL) Podcast with Wray Manning and Uday Sachdeva as they discuss topics that touch and titillate the heartstrings on a weekly basis.
NOTE: if you find the audio strange, I am trying my best.. but PLEASE let me know!! i would love to hear from you guys about the audio quality


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PRESS RELEASE – Top teams in the U12 and U14 divisions of the Chevrolet Robbie Webber Youth Soccer League Spring season, as well as a trio of U17 all-girls teams, will lace up their cleats this weekend against visiting teams from the Philippines.
The 2013 Pacific Summer Invitational tournament begins at 4 p.m. Friday with an opening ceremony at the Guam Football Association National Training Center. Matches in all three divisions kick off at 5 p.m. on both the upper and lower turf pitches. The round-robin matches continue Saturday beginning at 9 a.m., with playoffs scheduled for Sunday.
"This tournament is an excellent opportunity for both the visiting players from the Philippines and Guam's youth soccer club teams," said Gideon Diestro, sports advisor for Kaizen Philippines, one of the organizers of the tournament. "The visiting teams are afforded the opportunity to play against some of Guam's top club teams in a world-class facility like the GFA National Training Center, and for Guam's youth club teams, it's an opportunity to play against their peers in the region in a tournament-style setting.
"I would like to thank GFA for assisting tremendously in realizing this youth tournament," Diestro added.
Opening matches in the U12 division will feature Guam's ASC Islanders against Guam's Tigers at 5 p.m., followed by Southville International School and Colleges from the Philippines playing the Wolverines at 6:30 p.m.
In the U14 division, Quality Distributors FC will open play against the Wings FC, followed by the Rizal Football Association (RIFA) U14 team against Community First Dededo Soccer Club at 7 p.m.
In the U17 all-girls division, Southville takes on Quality Distributors FC 1 first, followed by Quality Distributors FC 2 playing against the Orange Crushers at 6:15 p.m.
The teams in the U12 division, as well as the U17 all-girls division, will play in a 7-a-side format, while the U14 division teams will compete in the full 11-a-side format.
RIFA is composed of Manila-based member schools that provides a venue for children to excel in the sport of football, the RIFA Facebook page states. Southville is an international school in Manila, Philippines, with its K-12 program accredited by the Western Association of Schools and Colleges (WASC), its Web site states. The school competes in RIFA leagues and tournaments, as well as the Metro Manila Girls Football Association leagues and tournaments for football, the school's Web site states.


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How is everyone surviving this cold and snowy winter so far? Here in Chicago land we are getting ready for some more -20 below temps along with another back to back to back snow storm! So while we will be snowed in, it will give me a chance to pack up all the Valentine papers and embellishments laying all over my work space.
I had ordered some new Graphic 45 papers and they just recently came in the post so I'm hoping to be able to play this weekend!
But as promised in my last post, here is one more card I had made from the Bo Bunny line, Little Miss. This was given to my daughter on her birthday.
Below are a couple valentine cards I had made - two of many but only took a picture of these two...not sure why I didn't photograph all of them...weird.
That's all for me. Time to start dinner and settle in to watch some favorite programs of mine on TV.
Good night
Wednesday, February 26, 2014
Sunday, February 9, 2014
celebrating 50
Happy Sunday to you all! It's been one snowy 2014 so far! I've never had so many "work from home" days in the 25 years I've been with the company I work for. They're big on 'safety first' so thanks to that motto, I can stay at home and fire up the laptop and not have to deal with the road conditions.
My snow blower has been used more this winter than the whole 3 years I've had it!
My family and I celebrated a milestone birthday last night. One of my younger sister's turned the big 50 today. Below is the card I made for her.
My snow blower has been used more this winter than the whole 3 years I've had it!
My family and I celebrated a milestone birthday last night. One of my younger sister's turned the big 50 today. Below is the card I made for her.
Pattern paper and journaling card is from Echo Park's "Sister" line. I jazzed it up by adding colored gems to the center of the flowers on the journaling card, along with some rose trim found in my stash. I adhered the journaling card and rose trim to some Stampin Up cardstock.
The month of January I was pretty much snowed in. Don't get me wrong, I love snow...as long as I don't have to drive in it! So to past the time away, I spent a lot of time cleaning and organizing the craft room once again. My sister had done the same thing to her craft space and was going to get rid of a lot of 'stuff' she wouldn't be using so of course, I took it all. One of the paper line's was by Bo Bunny - "Little Miss". Here are a few cards I made from that line.
Pattern paper and sticker is Bo Bunny. Doily is from Wilton, die cut flowers and felt are from Papertrey Ink. I punched the leaves from a Stampin Up punch and added pink pearl gems to the centers of the flowers.
Same Bo Bunny paper and journaling card, Wilton doily, ribbon from Stampin Up. There is a couple more cards I made but I'll wait to post them next time. One of them I made for my daughter who is celebrating a birthday next Sunday! Until then....
Have a great week ahead.
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Let's see, I'm 46, fairly-fit (certified as personal trainer), intelligent (720 GMAT), youthful (look younger, dress well, tech literate), and vibrant, and I do fear aging. My biggest concern is the loss of intellect that accompanies age. I haven't had cosmetic surgery, but I am not averse to it.
Caring about one's appearance is not ageist, and I imagine it is more often the anxiety of someone that is youthful and attractive.
Personally, ageist comments have come from 20-somethings, not older people or managers. According to a study, management typically has a higher regard for older workers, in terms of productivity, as well as with people skills, although managers also think younger workers can be more analytical.
On an intellectual level, older people tend to be neurologically slower, although the top few percent will increase their IQ up until the early 40's. After that, there is a typical, slowing and loss of intellect.


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Appearance Options
The Appearance tab in the Display Properties box allows you to adjust the style of windows and buttons, the color scheme, and font size. A preview of those settings appears on the upper half of the box as you can see in Figure 211.
The effects button configures transition effects, the appearance of screen fonts, icon edges and size, shadows under menus, and similar details24. The advanced button allows configuration advanced appearance items including but not limited to Desktop, 3D Objects, Active Title Bar Caption Buttons, Icon Spacing Scrollbar, and Window.
24. http://www.microsoft.com/enable/training/windowsxp/uselargeicons.aspx?v=t
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Innaxis is currently seeking for Data Scientists and Engineers to join its research and development team based in Madrid, Spain. Talented and highly motivated individuals who want to pursue and lead a career outside of the more mainstream, conventional alternatives. Individuals with a great dose of imagination, problem solving skills, flexibility and passion are encouraged to apply.
- As a Data Engineer, you will help the team to design and integrate complete solutions for Big Data architectures; from data acquisition and ETL processes until storage and delivery for analysis, using the latest technologies and solutions for the ultimate performance.
- As a Data Scientist, you will mainly assist the team to understand, analyse and mine data, but also to prepare and assess the quality of such. You will also develop methods for data fusion and anonymization. Ultimately your goal will be to extract the best knowledge and insights from data, despite technical limitations and committing with regulatory requirements.
About Innaxis
If not unique, Innaxis is at most not conventional: it is a private independent non-profit research institute focused on Data Science and its applications: most notoriously in aviation, air traffic management and mobility, among other areas.
As an independent entity, Innaxis determines its own research agenda and has now a decade of experience in European research programs with more than 30 successfully executed ones. New projects and initiatives are evaluated continuously and open to new opportunities and ideas proposed within the team.
The Innaxis team consist on a very interdisciplinary group of scientists, developers, engineers and program managers, together with an extensive network of external partners and collaborators, from private companies to universities, public entities and other research institutes.
Skills wanted
Our team work very closely on a daily basis,
- University degree, MSc or PhD on Data Science or Computer Science, or related field provided all other requirements are met.
- No professional experience required, although it might be positively evaluated.
- Proficient in a variety of programming languages, for instance: Python, Scala, Java, R or C++ and up to date on the newest software libraries and APIs, e.g. Tensorflow, Theano.
- Experience with acquisition, preparation, storage and delivery of data, including concepts ranging from ETL to Data Lakes.
- Knowledge of the most commonly used software stacks such as LAMP, LAPP, LEAP, OpenStack, SMACK or similar.
- Familiar with some of the IaaS, PaaS and SaaS platforms currently available such as Amazon Web Services, Microsoft Azure, Google Cloud and similar.
- Understanding of the most popular knowledge discovery and data mining problems and algorithms; predictive analytics, classification, map reduce, deep learning, random forest, support vector machines and such.
- Continuous interest for the latest technologies and developments, e.g. blockchain, Terraform,
- Excellent English communication skills. It is the working language at Innaxis.
- And of course, great doses of imagination, problem solving skills, flexibility and passion.
The successful candidate will be offered a Innaxis' position as a Data Scientist or Data Engineer, including a unique set of benefits:
- Being part of a young, dynamic, highly qualified, collaborative and heterogeneous international team.
- Great flexibility in many aspects -including working hours, compatibilities and location- and most excellent working conditions.
- A horizontal hierarchy, all researchers' opinions matter.
- Long term and stable position. Innaxis is steadily growing since its foundation ten years ago.
- A fair salary according to the nature of the institute and adjusted to skills, experience and education with continuous revision.
- Independence, as a non-profit and research-focused nature of Innaxis, the institute is driven by different forces than in the private sector, free of commercial and profit interests.
- The possibility to develop a unique career outside of mainstream: academics, private companies and consulting.
- No outsourcing whatsoever, all tasks will be performed at Innaxis offices.
- An agile working methodology; Innaxis recently implemented JIRA/Scrum and all the research is done on a collaborative wiki/Confluence.
Interested candidates should send their CV, a research interest letter (around 400 words) and any other relevant information supporting their application to firstname.lastname@example.com You will be contacted further and a personal selection process will start.


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SAP is gearing up to tackle another spot along its connected car roadmap for long-term sustainability.
Announced amid the SAP Automotive Forum in Leipzig, Germany this week, the software giant is teaming up with Toyota and VeriFone on a project that could help in the reduction of fuel consumption — all thanks to a few apps and extra data.
The collaboration consists of an in-car display, which can actually be visualized from a panel built into the car or simply on a mobile device. The enhanced dashboard on the dashboard can then bring up at-a-glance stats on fuel levels while tapping into location-aware information, such as nearby gas stations and personalised coupons.
The software and data processing behind the panel will be based on SAP's flagship HANA Cloud Platform, using Bluetooth Low Energy (BLE) to connect to nearby devices. Toyota will be responsible for the telematics data while VeriFone will provide point-of-sale technology.
Right now, the Toyota-VeriFone collaboration as well as a similar innovation investment with Volkswagen AG announced today are still in prototype testing mode.
This week's news follows a few other developments in June targeting the automotive industry through an amalgam of cloud, mobile and big data products.
SAP previously unveiled an electric vehicle program designed to encourage employees to lease electric cars.
SAP reps noted that the company's own car count stood at approximately 22,000 last year, accounting for nearly a quarter of the corporation's entire CO2 emissions.
Along with financial incentives to move the electric car adoption along, SAP also plans to plant more charging stations powered by renewable electricity sources for employee use.
By 2020, SAP asserted it plans to achieve the following goal: one in five company cars will run on electrically-powered engines.
This article has been extracted from http://www.zdnet.com, please click on this link to read the article in full http://www.zdnet.com/sap-revs-up-connected-car-drive-with-toyota-verifone-7000031077/
Montash is a multi-award winning, global IT recruitment firm. Specialising in permanent and contract positions across mid-senior appointments which cover a wide range of industry sectors and IT functions, including:
ERP, BI & Data, Information Security, IT Architecture & Strategy, Scientific Technologies, Demand IT and Business Engagement, Digital and E-commerce, Infrastructure and Service Delivery, Project and Programme Delivery.
With offices based in London, Montash has completed assignments in over 30 countries and has appointed technical professionals from board level to senior and mid-management in permanent and contract roles.


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After the jump, let's have a look at the prizes:
|This is the A prize. It takes batteries, though I can't tell what its function is.
|Prize B...
|...is a Gundam tissue paper holder. Awesome!
|Coat hanger for prize C!!
|Here's a handy card case.
|Tooth brush holder for Prize F. Yeah, the F prizes are usually down there on the desirability scale.
|Now this is nice, and it's the lowest prize - G. It's a Gundam-shaped ice cube maker!!
|Now you can have your favorite beverage on the rockets!


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Welcome to Bishara's blog pages, where the latest news about our sims and their development are published. Bishara Resorts is a project of SecondLabs, Dumisani Ah's creative group for the development of Second Life projects with a real world conservation edge. The Resort currently consists of 6 sims:
Bishara Island, our environmental and commercial sim, where you can dive, surf, sun, lounge, golf, bowl, and generally turf out on shopping in our mall. This was our very first sim in Second Life, a month after joining SL for the first time 🙂
Bahati Island, our very first residential / commercial sim, where residents own private islands amongst the beautiful Bishara reefs, teeming with sealife. If you are interested in living here, but cannot afford the current land prices, Bahati Apartments are open for rent and you are most welcome to live here for L$200 per week, at 200 prims per apartment.
Mandhari Island, our first privately designed sim by Jackson Mulberry, forms the volcanic centre of the Resort and is a residential sim only. Come and live in a volcanic crater, see the beautiful developing reef, and and have some first hand experience sailing the sailing course around the island.
Majini Island means in the water, and here you will find EPIC 3 waves by Heather Goodliffe. Come and surf the best waves in Second Life and enjoy living on the edge! Currently the premiere island surfing location in Second Life with the wave running the full sim, and the sim itself is a full specification class 5 sim, not a void.
The latest two islands have now been added as well, WOOOOTTTTT (May 2008)
PEPO Island or the Island of Winds is our premiere windsurfing sim with fluffies and pipes, as well as the very first unbelievable night surfing sim in Second Life, with phosphorous waves and beautiful colors to surf on. You have to see this to believe this!
WIMBI Island or the Island of Waves is our adventure surfing sim with some exciting options to your standard surfing day in SL 🙂 Come and try your luck with our fluffies and pipes.
Generally the resort was designed to draw SL residents to the wonders of the sea and to realize just how amazing RL's own reefs can be. We support REEF.ORG through a marketing program in-world, even though we do not have any direct connection with them. They are a fantastic conservation organization with a small budget and team taking on the huge task of preserving the non-coral reefs of the world, as well as their original focus, reservation through global fish counting exercises. Log onto their website for more info.
Come and enjoy our world! Most of the activities are completely free, and join the Bishara group if you need to use our equipment on the beach. For bowling there is a L$20 fee per play, but L$10 is paid back to you for a good set of frames, and you stand to win the pooled reward for each lane! Golf is subject to SL physics, but stick to learning how to play and the game will be most rewarding.
Be my guest, in a Resort where island living comes with style!
Who is Dumisani Ah? In RL his name is Johan de Lange, husband of one wife, father to a beautiful baby boy!