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Writing is an act that assists in the efforts toward balance. And it's beginning to look a lot like August!
New Readers
A few church members are finding out about my blog, and I welcome them — and more — to these pages. I don't say much here about my work. Sometimes I mention it, but the general absence is intentional. This is a personal journal of some items and events that help me stay focused on what is really important…or what makes me wonder. It is not a blog by Rev. Patterson, but a blog by Steve, a Jesus-follower guy who loves life and real people. I hope my new readers will find something here that touches real places within them, and that they will add their commentary when it feels right.
Come in. Have a seat. Let's immerse ourselves in something profound, or funny, or silently, incomprehensibly Christlike between us.
Check Out the Latest Blog I've Linked
It is primarily nature-based and originates from someplace in the Carolinas. The author goes by the name of Cedrorum, and his educational insights about natural events and entities are worth reading. I think you'll enjoy Mutual Causality.
In Case Some Clarification Would Help
I would like to bring you in on an underscored necessity of my life as a follower of Christ. About six years ago I wrote a sermon entitled Achieve New Balance and have been blessed to present it for several different churches. I won't repeat the whole sermon here, but the primary thrust of it comes in the form of a visual illustration. It is so much more difficult to describe a sermon than it is to preach one, but I'll try, because the truth of it continues to show itself crucial. And I've come to realize that there may be some who misunderstand what balance is.
Imagine or perform this task: Take an umbrella and hold it horizontally. Place a finger or the edge of your hand under the middle of it, and try to balance it. It's difficult. Imagine that at one end are the interests of Christ, and at the other end are the interests of the world or self, and this is what you're tring to balance. Good luck! The two ends moving in opposite directions with your finger as the pivot are hard to control. Even if you do get the two sides to behave and stay horizontal, a slight movement will upset the arrangement and it starts to topple again.
The problem is that we forget a mighty-but-quiet truth that John (the Baptizer) taught anyone who happened to be listening. John 3:30 — "He must increase, but I must decrease."
Go back to that umbrella that you were trying to balance. Pick it up and hold it horizontally again. Imagine again that the issues and interests of Christ are at the handle end and the issues of self interest are at the opposite tip end. Now, physically apply John's declaration to it. Raise (increase) the handle end up, while lowering (decreasing) the tip end. The umbrella remains increasingly unstable UNTIL the instrument is completely vertical. Place your finger under the tip end, and you can hold the umbrella in balance like that all day long! A pictoral of a Christian's life in balance would look like this umbrella — the character of Christ taking full precedence above the values of this world and interests of self.
Now, take your eyes away from Christ at the top and watch the bottom end of the umbrella where your own stuff is. What happens? The umbrella keeps moving but you can't see the movement until the fall is too far to stop. Only when the eyes remain on the top can the finger holding the bottom react to the movement and keep the device in balance. But the bottom must move with the top, staying underneath, submitted, or the thing comes crashing down.
The way we are built, we cannot look two places at one time (and similarly the heart cannot love/serve two masters). While the umbrella is horizontal, we can only occupy ourselves with the interests of Christ or with the interests of the world; it can't be both. And even if balance could be achieved in this position, the visual message it gives is that Christ and self/world are on the same level of importance — not a pleasing statement to a God who wants no competitors! When the umbrella goes vertical, our attention to Christ at the top takes care of the entire outfit. Balance is achieved when we watch the promises of Christ rather than the insecurities of self.
That's what balance is. It's about the top and Who occupies it. It's not about right or left. It's not remotely related to political correctness. I like the fact that my particular denomination has been referred to as occupying the "extreme center." (Not that it's always true, but it sure sounds good.) Nothing is more extreme than balance, and balance can only be known if Christ is at the center of one's vision, trust, aspiration, passion, ethics, action.
This is balance, not some tippy equalibrium that is obliterated by movement…but an upright, eye-locking dance that thrives in movement. It is kinetic and cathartic, holy and devastating, astringently disallowing the greasy grip of religion any hold that would choke away the Spirit's breath. He must increase, but I must decrease. Anything else is not balance for a Christian. And anything not balanced is a faith too off-center and scary for me.
No, it's not a perfect illustration, but that's good, because then it might be an idol. And too many of those already hound gentle souls who are just trying to follow.
Thank you for reading. Allow me to clarify further if I've raised additional questions.
A Break
Something happened yesterday that had not happened in a long time: I did not write in my journal. For 935 consecutive days, I wrote in my black Moleskine notebook, filling several along the way. But yesterday, I did not.
What began as an attempt to journal something every day of the year stretched to more than two-and-a-half years. During previous attempts, a calendar year would go by with two or three or just one day unrepresented by something written.
Usually when I have missed a day like that, it happens because of something good distracting me from the pattern of the pen. And I notice it with a small jolt soon after midnight. That's the way it was on December 25, 2005, the last time I missed. I became aware of the lost entry at 12:12 AM on Dec. 26. And I noticed yesterday's miss at 12:14 AM today.
So what was the good thing that distracted me from the habit? It was a day full of diverse activity — church work and personal errands. Lunch at home rather than on the road cost me a usual writing moment. During the evening — another time when journaling gets its chance — I decided to watch episode 3 of Lonesome Dove, borrowed from a friend church member who declares it the best western ever made. As I watched, I paused for a phone call from my sister, Jill, and that fun conversation lasted a long while. Later, a little deeper into the movie, Victor called, and we hunkered around our usual subjects of disc golf, computers, non-standard music, and people encounters. All of that still left me plenty of time to write, as I remarked to him that it was 11:00 before saying goodnight. But the movie came back on, and I was back in the dust and spirit of Nebraska, Wyoming, and Montana.
So I missed. And I laughed about it. Because I was full enough to not notice the time.
Kayaking on the Intracoastal Waterway
It was Oak Island, North Carolina, July 4, and the tide was low.
Popular place + holiday people + less water = heavy wave action.
But the day was beautiful anyway, and the excessive boat traffic didn't keep us from having a good time on the water.
Debra, and her wild rose Calypso.
A new bridge is being built to the west end of Oak Island, so there will be easier access for more people soon.
Having to keep close to shore to stay out of the motorboat lanes, our paddles were often scraping the bottom. Also, because we were in such shallow water, when someone may or may not have flipped over while trying to splash the other paddler, getting back in was not too difficult for her.
All this grass is completely covered by water when the tide is high. And there were LOTS of fiddler crabs here, moving like tiny herds of weird, crunchy buffalo across their sandy plain.
I'm still enjoying the Q700X. The rudder cable (cord) is wearing and may have to be replaced soon. If you have any questions about my kayak or the QCC experience, write and ask, or just click on the QCC link in the right sidebar.


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Every minute, of every day
So many things I want to say
But time gets in the way
& It's my heart that has to pay
Cause where I'm standing now
I don't know where to go
Maybe some things in this life
I'm just not meant to know
I'm standing on the edge
The water's getting deep
Maybe I'll make it alright
If I just take the leap
Cause my mind is telling my heart,
"Baby it's time you let this go.."
I'll never leave where I am
If I keep telling myself so
Cause the waves may crash against me
And the waters may be cold
But unhappy is no way to be
It's no way to grow old
And I'll never be anything different
If I don't decide to be
Time to dive in head first,
And set my soul free.
Holly Lee
Thursday, May 28, 2009
Another poem
Every minute, of every day
Posted by Hollymark at 5:54 PM
2 comments:
- Deanna said...
Very heart-filled poem, Holly! I wish you all good things and most importantly, happiness:D
- May 28, 2009 at 5:59 PM
- Anonymous said...
I found you by clicking "Etsy" in my "interests" links on Blogger and decided to "follow" you. I would like to invite you to my new blog, "Dish."
I would love it if you would tell us something you heard today, and something you did to make yourself heard today, at this post here:
See you,
- June 14, 2009 at 11:07 AM

subset: commoncrawl


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1. Smith On Arkansas� Future
Arkansas hasn�t enjoyed a season to remember in 2012. But Arkansas coach John L. Smith said Wednesday during the Southeastern Conference coaches teleconference it won�t take long for the Razorbacks to regain their spot as a staple in the top 25.
�Definitely. Why not? I mean, my goodness. It�s going to happen,� Smith said.
Arkansas (4-7, 2-5 in SEC) spent most of 2009 and 2010 ranked in the top 25 and among the top programs in the conference under former coach Bobby Petrino. The Razorbacks have stumbled on hard times this fall, though, entering the season finale against LSU with its worst record since the 2008 team finished 5-7.
Arkansas is in danger of losing eight games for the first time since 1990. But Smith is confident the �overall state of the program is going to be fine� because of a freshman class that includes key contributors like running back Jonathan Williams, cornerback Will Hines, receiver Mekale McKay and linebacker A.J. Turner.
�It�s going to take some time, so understand it�s going to take a year or so,� Smith said. �But these young guys, particularly this freshman class, is a great class. You just have to continue to recruit, continue to coach, and things are going to be fine.�
Smith isn�t expected to be around to see it after working under a 10-month contract this season. Arkansas athletic director Jeff Long is searching for the program�s long-term solution and, while not ruling anything out, won�t keep Smith in consideration.
But Smith, who said he was going to be overly emotional this week as he prepared for the final game, said Arkansas will be fine no matter who is hired.
�The greatest thing you have going is you have great facilities going, you have a program here, you have a great administration, you have great fans that are the best I�ve ever seen,� Smith said. �With all those positive things, it�s a positive outlook.�
2. Hamilton Accepts Invitation
Arkansas receiver Cobi Hamilton has accepted an invitation to play in the 2013 Senior Bowl. The Senior Bowl announced the news Wednesday via Twitter.
Hamilton has set school records in receptions (80) and receiving yards (1,237). He will get a chance to add to those totals Friday, when the Razorbacks play LSU.
The Senior Bowl, held in Mobile, Ala., is regarded as one of the top events leading into the NFL Draft. Teams are coached by NFL staffs, while general managers, coaches and scouts from every organization will be in attendance all week. Hamilton is regarded as one of the top receivers in the 2013 draft class to date.
The 2013 Senior Bowl will be played on Jan. 26 in Mobile's Ladd-Peebles Stadium. The game will be televised by the NFL Network.
3. Miles-Nash Limited
Smith said senior Colton Miles-Nash should be able to play in his final game Saturday, but will be limited because of a knee injury. Miles-Nash was injured early in the 45-14 loss to Mississippi State last Saturday.
�Is he going to be able to play defense? No,� Smith said. �Hopefully he can do all the special teams duties. That would probably be the most we can expect. But he wants to play in the worst way. So we�ll make every effort to make sure that he does.�
4. Miles Eager For Fayetteville
Arkansas and LSU will play in Fayetteville for the first time since 1992, when the Razorbacks first entered the SEC. So LSU coach Les Miles said his team, which has grown accustomed to playing the Hogs in Little Rock, is eager for a new venue.
�Having played in Little Rock before, I recognize there�s a very strong home field advantage for them there,� Miles said. �They have a very large population base of Arkansas and friends. Being in Fayetteville will be a new experience for me, but I recognize that�s the home of the university and we�re looking forward to playing there and seeing a very quality SEC venue.�
� Robbie Neiswanger � Arkansas News Bureau


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Whether you have been given a puppy as a gift or you have recently adopted a dog from a shelter, you want your new pet to feel as welcome as possible in your home. Dogs are no different from people in that any change in environment will be an experience that requires adjustment. Here are a few ways to make your dog feel welcome in your home:
1. Prepare a sanctuary or safe haven. Allow your dog to feel secure by preparing a special place in your home just for him. This can be a crate, an enclosure, or anyplace nice and warm that will allow him to feel safe.
2. Schedule a visit to a veterinarian Jacksonville. Your new dog's health and well-being should be your number one priority. A healthy dog is a happy one.
3. Give him his space. While wanting to spend as much time as possible with your new dog is understandable, give him his space. Eventually, he will get accustomed to his new environment and warm up to you.


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SWARTHMORE, Pa. – Brett Cohen posted his fifth double-double of the season and the Fords had the final shot of the game, but that missed 3-pointer rimmed out leaving Haverford College's men's basketball team heart broken as it fell to host Swarthmore College, 62-59, in Centennial Conference action Wednesday night in Tarble Pavilion.
Cohen posted a game-high 19 points, pulled down 11 rebounds and added three assists, two steals and four blocks to his night. The junior's efforts weren't quite enough for the Fords (5-11, 3-8 CC) who twice, once in each half, rallied from double-digit deficits that the Garnet (5-13, 4-7) had raced out to in front of their home crowd.
With 49 seconds left in the game, Cohen's layup and successful free throw from a foul on the shot pulled Haverford to within a point, 60-59, nearly completing a come-back from 13 points down at the 9:34 mark.
Swarthmore's Will Gates missed a jumper at the other end on the ensuing possession and Gibby Graves pulled down the rebound to provide the Fords with a chance for a go-ahead basket. After a Haverford timeout, Cam Baker's contested jumper was just off the mark with seven seconds remaining. The Garnet's Jay Kober came down with the rebound and was fouled immediately. He sank both free throws to push the Swarthmore advantage to three.
There were still five seconds on the game clock, though, and the Fords pushed the ball up court and set up a tying 3-point attempt by Louis Cipriano. Neither luck nor fate were with Cipriano and Haverford on the shot as it collected only the rim allowing the home team to sneak away with the victory.
Despite the two misses at the end of the game, Haverford had a solid night from the floor, hitting 45 percent of its shots, out shooting Swarthmore which converted only 37 percent. The Fords' night was aided by scoring 46 of their 59 points from in close within the paint.
Along with Cohen's double-digit night, Baker's 13 points, 11 more from James Levine and Lucas Colombo's 10 points added to the Fords' efforts in the offensive end. Kober and Gates each registered 17 points to lead Swarthmore in scoring.
Swarthmore's victory earns the tying half-point in the annual Hood Trophy race between the two rival institutions. The score stands knotted at 3.5–3.5 after Wednesday night's game.
With 13 more rebounds Cohen will become just the 14th player in program history to accumulate 500 rebounds.
Haverford's challenges don't get any easier with their next game as it welcomes Centennial-leader Franklin & Marshall College to Gooding Arena on Saturday afternoon. Tip-off is scheduled for 3 p.m., following the women's game between the same two combatants.


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- Research article
- Open Access
- Open Peer Review
TFPI-2 is a putative tumor suppressor gene frequently inactivated by promoter hypermethylation in nasopharyngeal carcinoma
- Shumin Wang†1,
- Xue Xiao†1,
- Xiaoying Zhou1,
- Tingting Huang1,
- Chunping Du1,
- Nana Yu1,
- Yingxi Mo2,
- Longde Lin1,
- Jinyan Zhang1,
- Ning Ma3,
- Mariko Murata2,
- Guangwu Huang1 and
- Zhe Zhang1Email author
© Wang et al; licensee BioMed Central Ltd. 2010
- Received: 15 June 2010
- Accepted: 9 November 2010
- Published: 9 November 2010
Epigenetic silencing of tumor suppressor genes play important roles in NPC tumorgenesis. Tissue factor pathway inhibitor-2 (TFPI-2), is a protease inhibitor. Recently, TFPI-2 was suggested to be a tumor suppressor gene involved in tumorigenesis and metastasis in some cancers. In this study, we investigated whether TFPI-2 was inactivated epigenetically in nasopharyngeal carcinoma (NPC).
Transcriptional expression levels of TFPI-2 was evaluated by RT-PCR. Methylation status were investigated by methylation specific PCR and bisulfate genomic sequencing. The role of TFPI-2 as a tumor suppressor gene in NPC was addressed by re-introducing TFPI-2 expression into the NPC cell line CNE2.
TFPI-2 mRNA transcription was inactivated in NPC cell lines. TFPI-2 was aberrantly methylated in 66.7% (4/6) NPC cell lines and 88.6% (62/70) of NPC primary tumors, but not in normal nasopharyngeal epithelia. TFPI-2 expression could be restored in NPC cells after demethylation treatment. Ectopic expression of TFPI-2 in NPC cells induced apoptosis and inhibited cell proliferation, colony formation and cell migration.
Epigenetic inactivation of TFPI-2 by promoter hypermethylation is a frequent and tumor specific event in NPC. TFPI-2 might be considering as a putative tumor suppressor gene in NPC.
- Promoter Hypermethylation
- Bisulphite
- CNE2 Cell
- Normal Nasopharyngeal Epithelium
- Bisulphite Genomic Sequencing
Nasopharyngeal carcinoma (NPC) is a rare disease in most parts of the world but is one of the most prevalent malignasnt tumors and the leading cause of death among all head and neck cancers in Southern China[1]. Although the major etiological factors, including genetic susceptibility, environmental carcinogens and Epstein-Barr virus infection, are well established, the complicated molecular basis of NPC development and progression remains unclear[1]. In addition to genetic alterations, epigenetic inactivation of tumor suppressor genes (TSGs) by promoter hypermethylation has been increasingly recognized as a key event for nasopharyngeal carcinogenesis[1–4]. Compared with the incidence of DNA mutation and deletion, that of aberrant DNA methylation of TSGs is high in NPC, which suggests a deep involvement of aberrant DNA methylation in this rare cancer[1].
Besides having a role as a diagnostic or prognostic bio-marker, hypermethylated DNA can also be used to reveal novel TSGs[5]. Identifying aberrant methylated genes may provide better understanding of the pathogenesis of NPC, thereby paving the way for the development of novel tumor markers and therapeutic targets. To discover novel TSGs in NPC, we conducted a genome-wide screening for genes downregulated by promoter hypermethylation. Analysis of an expression microarray with RNA from 2 NPC cell lines before and after treatment with a demethylating agent 5-aza-2'-deoxycytidine (5-aza-dC) revealed the transcriptional levels of TFPI-2 in both cell lines significantly upregulated after demethylation(unpublished data). Thus, TFPI-2 might be a target gene with expression suppressed by promoter hypermethylation in NPC.
The human TFPI-2 gene is located on chromosome 7q22 and encodes a 32-kDa Kunitz-type serine protease inhibitor that negatively regulates the enzymatic activity of trypsin, plasmin, and VIIa-tissue factor complex[6–8]. TFPI-2 was found to be abundantly expressed in various normal human tissues, including seminal vesicles, colon, stomach, brain, pancreas, oesophagus and liver[9–13]. Several studies have provided suggestive evidence that TFPI-2 is inactivated or absent during tumor progression. In addition, expression of TFPI-2 decreases with an increasing degree of malignancy[10]. Besides gene locus deletion and aberrant splicing, the mechanism responsible for TFPI-2 down-regulation in tumor cells has been majorly attributed to promoter hypermethylation[14–16]. The promoter region of TFPI-2 contains a CpG island spanning exon 1 and the 3 transcription initiation sites[17]. This region harbors sites for several transcription factors, including SP1, activiting enhancer-binding protein-1 (AP-1), NF-kB and NF-kB-like site, and lymphoid transcription factor-1 (Lyf-1)[18]. Transcriptional silencing by promoter hypermethylation of TFPI-2 was observed in some human cancers[9, 19, 20]. Recent studies also suggest TFPI-2 may function in the maintenance of the stability of the tumor environment and inhibit the growth of neoplasms, thus act as a candidate TSG with important roles in carcinogenesis and metastasis in human cancers[9, 12].
In this study, we evaluated the transcriptional levels and methylation status of TFPI-2 in NPC cell lines and analyzed the methylation status of TFPI-2 in a set of NPC primary tumor biopsies. We further addressed the TSG properties of TFPI-2 in NPC by a series of functional experiments. Several lines of evidence support our hypothesis that TFPI-2 is epigenetically inactivated in NPC by promoter hypermethylation and plays a role as a TSG in NPC tumorigenesis.
NPC cell lines, primary tumor biopsies and normal nasopharyngeal epithelia (NNE)
Six NPC cell lines (CNE1, CNE2, TW03, C666-1, HNE1, and HONE1) were maintained at 37°C in the appropriate medium. The study was approved by the ethics committee of Guangxi Medical University. In total, 70 NPC primary tumor biopsies were collected from Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University (Nanning, China), with informed consent from donors, as previously described[2, 3]. Diagnoses were established by experienced pathologists according to the World Health Organization (WHO) classification. We included 12 normal nasopharyngeal epithelial tissues obtained by tonsillectomy as normal controls. Biopsy samples were stored in liquid nitrogen before DNA or RNA extraction.
Semi-quantitative RT-PCR
Total RNA of NPC cell lines and normal NNE was isolated with TRIzol reagent (Invitrogen, USA). First-strand cDNA was synthesized with M-MLV reverse transcriptase (Promega, USA) according to the manufacturer's instructions. Two micrograms of total RNA was used for each reaction. Primer sequences for TFPI-2 cDNA were designed according to Norihiro et al, generating a 209-bp PCR product: TFPI-2-RT-forward: CCAGATGAAGCTACTTGTATG and TFPI-2-RT-reverse: GCACATGCACGTTTGCAATC [21]. PCR was carried out in a total volume of 20 μl. The PCR mixture contained 250 pmol of each primer, 250 pmol deoxynucleoside triphosphate, 1 × PCR buffer, one unit of ExTaq HS polymerase (Takara, Tokyo), and 2 μl cDNA. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was amplified from the same cDNA sample as the internal control. The primer sequences for GAPDH cDNA were: GAPDH-RT-forward: AAGCTCACTGGCATGGCCTT, and GAPDH-RT-reverse: CTCTCTTCCTCTTGTGCTCTTG, generating a 375-bp PCR product. PCR conditions were 94°C for 30 s, 58°C for 30 s (TFPI-2) or 60°C for 30 s (GAPDH), and 72°C for 30 s, 33 cycles for the TFPI-2 gene and 24 cycles for the GAPDH gene. These cycle numbers fell into the exponential range of PCR amplification. The amplified PCR products were then identified on 2% agarose gels. Images of ethidium bromide-stained agarose gels were acquired with a CCD camera (Bio-Rad, USA), and semi-quantitative analysis involved use of the Quantity-one software, v4.4.0 (Bio-Rad, USA).
Sodium bisulphite modification of genomic DNA
High-molecular-weight genomic DNA was extracted from cell lines and biopsies by a conventional phenol/chloroform method. The sodium bisulphite modification procedure was as described [22] with slight modification. In brief, 600 ng of genomic DNA was denatured in 3 M NaOH for 15 min at 37°C, then mixed with 2 volumes of 2% low-melting-point agarose. Agarose/DNA mixtures were then pipetted into chilled mineral oil to form agarose beads. Aliquots of 200 μl of 5 M bisulphite solution (2.5 M sodium metabisulphite, 100 mM hydroquinone, both Sigma, USA) were added into each tube containing a single bead. The bisulphite reaction was then carried out by incubating the reaction mixture for 4 h at 50°C in the dark. Treatments were stopped by equilibration against 1 ml of TE buffer, followed by desulphonation in 500 μl of 0.2 M NaOH. Finally, the beads were washed with 1 ml of TE buffer and directly used for PCR.
Methylation-specific PCR
The methylation status of the TFPI-2 promoter region was determined by methylation-specific PCR. Primers distinguishing unmethylated (U) and methylated (M) alleles were designed to amplify the sequence of TFPI-2 from - 44 to + 60 bp relative to the transcription start point[21]: TFPI-2-M-forward: TTTCGTATAAAGCGGGTATTC; TFPI-2-M-reverse: ACGACCCGCTAAACAAAACG; TFPI-2-U-forward: GGATGTTTGTTTTGTATAAAGTG; TFPI-2-U-reverse: AAACATCCAAAAAAACACCTAAC. Each PCR reaction contained 20 ng of sodium bisulphite-modified DNA, 250 pmol of each primer, 250 pmol deoxynucleoside triphosphate, 1 × PCR buffer, and one unit of ExTaq HS polymerase (Takara, Tokyo) in a final reaction volume of 20 μl. Cycling conditions were initial denaturation at 95°C for 3 min, 40 cycles of 94°C for 30 s, 54°C (M) or 60°C (U) for 30 s, and 72°C for 30 s. For each set of methylation-specific PCR reactions, in vitro-methylated genomic DNA treated with sodium bisulphite served as a positive methylation control. A water blank control was also included. PCR products were separated on 2% agarose gels, stained with ethidium bromide and visualized under UV illumination. For cases with borderline results, PCR analyses were repeated.
Bisulphite genomic sequencing
Sodium bisulphite-modified DNA was subjected to PCR with primers designed to amplify nucleotides from - 178 to + 166 bp relative to the transcription start point of the TFPI-2 gene. Primers sequences were designed according to previous publication by Norihiro et al. [21] TFPI-2-BISQ-forward: AGGTAGGTTTAATTTTTTAATTTG and TFPI-2-BISQ-reverse: TACCTATTAACTCCTAAACAAC. PCR was carried out in a total volume of 20 μl, containing 20 ng of sodium bisulphite-modified DNA as template, 250 pmol of each primer, 250 pmol of deoxynucleoside triphosphate, 1× PCR buffer, and one unit of ExTaq HS polymerase (Takara, Tokyo). Cycling conditions were as follows: initial denaturation at 95°C for 3 min, 40 cycles of 94°C for 30 s, 58°C for 30 s, and 72°C for 30 s, and a final extension at 72°C for 5 min. PCR products were then gel-purified and cloned with use of the pMD18-T Vector (Takara, Tokyo) and JM109-competent E. coli cells. Colonies were grown on agar plates, and 5 colonies of each sample were randomly selected. Plasmids were then isolated and purified. Sequencing were carried out using the BigDye terminator cycle sequencing kit 3.0 (Applied Biosystems, USA) on an ABI 3100 sequencer according to the manufacturer's guidelines.
5-aza-dC treatment
Three NPC cell lines (CNE1, CNE2 and C666-1) were treated with the methyltransferase inhibitor 5-aza-dC. An amount of 2 × 105 cells were seeded on 6-well plates and incubated for 96 h with 10 μM 5-aza-dC. The medium containing the drug was replaced every 24 h. RNA was isolated, and subsequent semi-quantitative RT-PCR was performed as described previously.
Vector construction and transfection
The full-length cDNA sequence of TFPI-2 was from Origene (USA) and subcloned into the pCMV-Tag3A mammalian expression vector (Stratagene, USA). Lipofectamine 2000 (Invitrogen, USA) was used to transfect the TFPI-2-containing plasmids and empty vectors into CNE2 cells, which lack TFPI-2 expression. Stable clones of TFPI-2 (CNE2-TFPI-2) or empty vector (CNE2 empty vector) were obtained by G418 selection (400 μg/ml) for 2 weeks. The expression of exogenous TFPI-2 was confirmed by RT-PCR.
Colony formation assay
An amount of 105 CNE2 cells was plated in 24-well plates 24 h before transfection. Lipofectamine 2000 was used for transfection according to the manufacturer's protocol. Cells were transfected with 2 μg pCMV-Tag3A-TFPI-2 plasmid or empty vector. Transfected cells were stripped and plated on 60-mm cell culture dishes 48 h after transfection. After selection by 400 μg/ml G418 for 2 weeks, Giemsa-stained colonies were photographed and counted by use of Quantitione software, v4.4.0 (Bio-Rad, USA).
Cell proliferation assay
The growth curves of CNE2 cells stably transfected with TFPI-2 gene (CNE2-TFPI-2) or empty vector (CNE2-empty vector) were monitored by cell counting. An amount of 104 cells of parent CNE2 cells, CNE2-TFPI-2 and CNE2-empty vector were seeded into 6-well plates. Cells were trypsinized and stained with a 0.4% trypan blue solution every 24 h. Dye-exclusive cells were counted by use of a hemocytometer.
Migration study
Cell migration was assessed by in vitro wound-healing assay. Stable transfectants of CNE2, CNE2-TFPI-2 and CNE2-empty vector were cultured on 35-mm dishes for 24-48 h until they reached confluence. A linear wound track was made by use of a sterile tip. Wounds were marked and scored in each dish. Plates were then washed twice with culture medium. Cells migrating into the wound were photographed under a phase-contrast microscope after 24 h incubation after wounding.
Annexin V-FITC/PI double-labeled flow cytometry
To determine apoptosis, the expression of Annexin V-FITC and exclusion of propidium iodide (PI) was detected by double-labeled flow cytometry. Cells transfected with empty vector and TFPI-2 were collected and washed with PBS 24 h after transfection, then resuspended in 100 μL binding buffer. Samples were incubated with 5 μL Annexin V-FITC in the dark for 10 min at 4°C, then the volume was adjusted to 500 μL with binding buffer. PI (5 μL) was added, and samples were incubated for another 10 min at 4°C. Fluorescence was measured with a flow cytometer (BD FACSCalibur, USA).
Statistical analysis
SPSS v11.5 (SPSS Inc., Chicago, IL) was used for statistical analysis. Association between methylated sample data and clinical pathological features of NPC patients were analyzed by Pearson chi-square test or Fisher's exact test. A p < 0.05 was considered statistically significant.
TFPI-2mRNA expression is frequently absent in NPC cell lines
TFPI-2promoter is hypermethylated in NPC cell lines and primary NPC tumors
CpG sites in the TFPI-2 promoter are heavily methylated in NPC cell lines and NPC biopsies
Transcription of TFPI-2can be restored by 5-aza-dC treatment
Clinico-pathological significance of TFPI-2promoter hypermethylation
Associations between TFPI- 2 promoter methylation and clinicopathological indices of NPC
Promoter methylation status
Number of patients
Statistic significance
< 60
≥ 60
Histological subtype
Keratinizing squamous cell carcinoma
Non-keratinizing carcinoma
Lymph node metastasis
TFPI-2suppresses cell proliferation and colony formation and inhibits cell migration in NPC cells
TFPI-2induces apoptosis in NPC cells
Previously, we analyzed expression microarray data before and after treatment with demethylating agent in 2 NPC cell lines and speculated that the TFPI-2 gene may be downregulated by promoter hypermethylation in NPC cells. TFPI-2, also known as placental protein 5 and matrix-associated serine protease inhibitor [7, 23], is a novel serine protease inhibitor[7, 24]. It was reported to be involved in tumorigenesis and metastasis in several types of cancers[24]. Suppression of TFPI-2 gene expression is frequently found in melanoma, liver and pancreatic cancer[9, 25, 26]. Downregulation of TFPI-2 mRNA and protein by promoter hypermethylation has been confirmed by RT-PCR, immunostaining, methylation specific PCR and bisulfate genomic sequencing [9, 27]. In this study, hypermethylation of the TFPI-2 promoter was detected in 4 of 6 (66.7%) NPC cell lines. We also showed a high frequency (88.6%) of TFPI-2 promoter hypermethylation in NPC primary tumor biopsies but not in the NNE tissues, which implied that transcriptional silencing of the TFPI-2 pathway might be involved in NPC tumorigenesis. As well, we found a high frequency of promoter hypermethylation of TFPI-2 in early stage (I and II) NPC, which indicates that this might be an early event in NPC carcinogenesis. In contrast to the high frequency of methylation of TFPI-2 in NPC cell lines and primary tumors, the absence of promoter hypermethylation in 12 histological NNE tissues showed that hypermethylation of TFPI-2 promoter is not necessary to maintain the normal phenotype in the nasopharyngeal epithelium. Thus, hypermethylation of TFPI-2 is a frequent and highly tumor-specific event in NPC. Such characteristics may suggest TFPI-2 methylation as a molecular marker for NPC.
By bisulfite genomic sequencing assay, we found methylation degrees ranging from 60% to 100% in most of the CpG dinucleotides in the TFPI-2-silenced NPC cell lines CNE2 and C666-1. A heavy degree of methylation was also observed in NPC primary tumors, despite the inevitable normal tissue contamination in the biopsy samples without micro-dissection. Moreover, TFPI-2 was completely restored by demethylation treatment in all 3 TFPI-2-silenced NPC cell lines. In this context, epigenetic inactivation of TFPI-2 by promoter hypermethylation is a major, if not the only, mechanism responsible for the loss of TFPI-2 expression in NPC.
Although promoter hypermethylation of TFPI-2 is frequently found in NPC, it is not associated with sex, age, nodal metastasis or cancer stage in our series. This finding is inconsistent with observations in pancreatic and non-small-cell lung cancer; a high methylation rate of TFPI-2 was reported in pancreatic cancer patients with liver metastasis[25]. In non-small-cell lung cancer, TFPI-2 promoter hypermethylation was frequently found in patients with late-stage cancer (stages III and IV) and with lymph node metastases[27]. These findings suggest that the prognostic value of promoter hypermethylation of TFPI-2 is tissue-specific.
The tumor-suppressive functions of TFPI-2 have been demonstrated in several human malignancies such as lung cancer, prostate cancer, glioma, melanoma and esophageal carcinoma. Ectopic expression of TFPI-2 protein in these cancer cell lines negatively regulates colony formation and inhibits cell proliferation[12, 27–30]. Additional studies have shown that TFPI-2 inhibits tumor-related angiogenesis and some members of the extracellular matrix (ECM) therefore implicates tumor invasion and progression[8, 12, 17, 29]. Degradation of the ECM is an essential process for tumor invasion and metastasis and involves various matrix-degrading proteinases. The most important proteolytic ECM enzymes are matrix metalloproteinases (MMPs), and upregulated MMP expression has been demonstrated to be strongly associated with the progression of malignancy in several types of cancer, including NPC[31, 32]. By inhibiting plasmin, TFPI-2 effectively decreases the activation of MMP-1, MMP-3 and MMP-9 and reduces the invasive potential of several cancer cell lines[9, 12, 33, 34]. In accordance with previous studies, we demonstrated that ectopic expression of TFPI-2 significantly inhibited cell proliferation, colony formation and migration in NPC cells. We also demonstrated that restoration of TFPI-2 induces apoptosis in NPC cells. All these evidence strongly implies that TFPI-2 is a putative TSG and plays a role in nasopharyngeal carcinogenesis.
Methylation-mediated inactivation is reversible, up-regulating TFPI-2 by demethylating agent may reverse the malignant phenotype of tumor cells. Therefore TFPI-2 can serve as a novel target for gene therapy in NPC treatment. On the other hand, TFPI-2 functions extracellularly as a secreted protein. Treatment with recombinant TFPI-2 protein inhibited tumor growth and metastasis of esophageal cancer[12]. Thus, TFPI-2 protein might be used directly as an anticancer drug. Further studies will be necessary to explore the great therapeutic potential of TFPI-2 in NPC and other cancer.
The present work demonstrated that epigenetic inactivation of TFPI-2 by promoter hypermethylation is a frequent and tumor specific event in NPC and TFPI-2 might be considering as a putatitive tumor suppressor gene in NPC.
This study was supported by grants from the National Natural Foundation of China (30660197, 30960416), Ministry of Education of China and Science & Technology Committee of Guangxi Province, China (0728052). Ministry of Education, Culture, Sports, Science, Japan(2240606).
Authors' Affiliations
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- Rao CN, et al: Novel extracellular matrix-associated serine proteinase inhibitors from human skin fibroblasts. Arch Biochem Biophys. 1995, 317 (1): 311-4. 10.1006/abbi.1995.1168.View ArticlePubMedGoogle Scholar
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- Ran Y, et al: A novel role for tissue factor pathway inhibitor-2 in the therapy of human esophageal carcinoma. Hum Gene Ther. 2009, 20 (1): 41-9. 10.1089/hum.2008.129.View ArticlePubMedGoogle Scholar
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- Dong JT: Chromosomal deletions and tumor suppressor genes in prostate cancer. Cancer Metastasis Rev. 2001, 20 (3-4): 173-93. 10.1023/A:1015575125780.View ArticlePubMedGoogle Scholar
- Sell SM, et al: Minimal interval defined on 7q in uterine leiomyoma. Cancer Genet Cytogenet. 2005, 157 (1): 67-9. 10.1016/j.cancergencyto.2004.06.007.View ArticlePubMedGoogle Scholar
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- Hibi K, et al: Methylation of TFPI2 gene is frequently detected in advanced well-differentiated colorectal cancer. Anticancer Res. 2010, 30 (4): 1205-7.PubMedGoogle Scholar
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- Nobeyama Y, et al: Silencing of tissue factor pathway inhibitor-2 gene in malignant melanomas. Int J Cancer. 2007, 121 (2): 301-7. 10.1002/ijc.22637.View ArticlePubMedGoogle Scholar
- Rollin J, et al: Expression and methylation status of tissue factor pathway inhibitor-2 gene in non-small-cell lung cancer. Br J Cancer. 2005, 92 (4): 775-83. 10.1038/sj.bjc.6602298.View ArticlePubMedPubMed CentralGoogle Scholar
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- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/10/617/prepub
Pre-publication history
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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In the financial climate nowadays, flooring is more affordable than 4 or 5 decades ago. Wood flooring is just of the most beautiful and durable flooring types you're ready to put in your house, but like any surface, it will require upkeep from time to time. Refinishing engineered wood flooring is a fantastic solution for the vast majority of homeowners. Engineered hardwood in Mississauga, ON, is an excellent choice.
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Engineered hardwood floors are constructed of a good bit of timber; engineered planks employ just a veneer of genuine hardwood. Strong hardwood flooring has existed the longest and has become the principal flooring alternative for centuries, and for many very good explanations. Our choice of pre-finished fantastic hardwood flooring provides you some finishes, widths, and alternatives to match your style, from rustic to contemporary.
If you are tired of the way that your flooring appears right now or are constructing a new residence, you should look at engineered hardwood flooring. Engineered hardwood flooring can be found in an endless number of colors, sizes, shapes, and finishes. Therefore there is no limit to the stunning, personalized designs to make. This flooring also supplies a thinner profile than many solid wood alternatives, which is a significant element in a place of your home where headroom can be difficult to find. Engineered flooring is another kind of flooring which you really shouldn't write off until you have done your homework on the matter. If you're planning to install engineered wood flooring, then you should be sure the area you reside in doesn't have high humidity or moisture levels.
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But I don't think it's the number one thing. Hands down, I think the number one thing is.... are you ready? It's...
After all, if you persistently do something wrong, that's not going to get you where you need to go, is it? And the only way to make persistence work is if you're teachable every step of the way.
Even when your critique partner tells you something you don't want to hear.
Even when it comes from someone "behind" you on this road we're all traveling.
Even when it's constructive criticism.
Even when you think you have it made.
Even when it comes along with a rejection from an agent / editor.
Even when you're a NYT bestseller.
Even when it means a major overhaul of your manuscript.
Even when it hurts your pride.
Even when it is ohmygosh so. very. hard. Because really, sometimes it just is.
ESPECIALLY when it's so. very. hard.
If you can stay teachable every step of the way, YOU WILL MAKE IT. You will reach your goals. And when you do reach your goals, it'll help you reach the next ones. And the next, and the next....


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Magnificent Ant Nebula
The Ant Nebula is one of the most striking planetary nebulae known. Planetary nebulae - whose name arises because most are spherical and looked like planets when they were first discovered through older, less powerful telescopes - are glowing structures of gas cast off by solar-like stars at the ends of their lives. The morphology of the Ant Nebula - a bright core, three nested pairs of bipolar lobes and a ring-like outflow - is so unique that it was nicknamed the 'Chamber of Horrors' of planetary nebulae in the late 1950s.Read more about it (and why it's in the news right now) here


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Mutton Korma or Mutton Kurma is a delicious non-vegetarian curry item of India mainly made with either goat meat or lamb. Korma (sometimes spelled Kurma or Khorma) has it's origin in the Mughal period. Today it is found all over the Indian subcontinent, prepared with a combination of ingredients including coconuts and spices.Korma is eaten by a large number of people in India because it is not too spicy and pungent It is consumed with rice or other entrés like chapatti, paratha etc. In India, mutton typically refers to goat meat, and this is the meat used in Mutton Korma in Tamin Nadu.
Cooking Time: 30 minutes
Servings: 3
Mutton: 250 grams
Onions: 2
Green chili: 3
Curd: ½ cup
Salt: ¾ teaspoon
Lime: ½
Coriander powder: 1 ½ tablespoon
Coriander leaves: one small bunch
Oil: 4 teaspoon
Clove: 5
Cinnamon: 2 small pieces
Ginger: 1″ piece
Garlic: 6 pods
Grated Coconut: 1 cup
Khus khus (poppy seeds): 2 teaspoon
Cashew nut: 10
Wash mutton and marinate in curd with salt for an hour. Slice green chili and onions. In a blender, grind one cinnamon piece, 3 cloves, ginger and garlic to a paste and set aside (paste 1). Separately grind grated coconut, khus khus, and cashew nut to a smooth paste and set aside (paste 2).
In a pressure cooker add oil and heat. Add 2 cloves and one cinnamon piece along with sliced onion and green chili and sauté till the onions turn translucent. Add the marinated mutton and fry till the curd is absorbed. Now add the chopped coriander leaves and coriander powder along with paste 1. Raise the flame to high and close the lid after adding enough water to cook the mutton. Putting on the weight (steam stopper), you can reduce the heat to low and cook for 15mts. Turn off the heat and wait. When the cooker is cold enough to open, bring it back on low heat, add the paste 2 and stir for a couple of minutes. Finally just before taking off from the stove, squeeze in lime juice.
Tips: If you like, you can add one or two potato and cook with the mutton.


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AC BeachFest Concert Series presents The Chainsmokers
July 29 | 12:00 pm - 10:00 pm
- Atlantic City Beach
- Arkansas Avenue & Boardwalk , Atlantic City, NJ 08401 United States
Additional Information
Atlantic City BeachFest Concert Series presents The Chainsmokers with special guests BAZZI on the Atlantic City Beach on Sunday, July 29th! We're giving you a chance to get YOUR tickets FIRST during an exclusive pre-sale on Thursday, March 19th from 10am until 10pm by using the password: RADIO! Tickets go on sale to the public on Friday, April 20th at 10am through Ticketmaster.com!


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World Book Day Costume Ideas
30th Jun 2017
By Tickled Pink 5 months ago 4029 Views 37 comments
World Book Day returns for its 20th year on March 2nd and school and nurseries across the country are encouraging kids to "Do Something Booky". World Book Day is a worldwide celebration of authors, books, illustrators and most importantly reading so choose a book and get involved. We've got all the details of what happens on the day, competitions and vital World Book Day Costume ideas to ensure your child looks the part.
Competition Update: We are giving two lucky winners the chance to win a Kids World Book Day Costume worth up to £24.99 so enter now. Closes Sunday 26th February and includes free guaranteed delivery for World Book Day. Enter at the bottom of the post.
What happens on the day?
The most popular way to celebrate World Book Day is to dress up as a character from your favorite book. This might be a hero or heroine from a book that you've read recently or a timeless book character. Harry Potter, Horrible Histories, Where's Wally and Disney are popular themes from modern literature whilst Roald Dahl, The Gruffalo, Robin Hood are timeless classics. AA Milne's Winnie the Pooh remains a firm favorite for younger children at nursery and pre-school. Remember to learn a little about the character you dress up as, because Book quizzes, matching characters to authors and literacy quotes are common activities on the day.
Costume Ideas
Roald Dahl is famous for creating characters including Charlie and the Chocolate Factory, crazy Willy Wonker, studious Matilda, the terrifying Miss Trunchbull and Fantastic Mr Fox. Following the release of The BFG (Big Friendly Giant) film last year, we are predicting that the young giant will be a popular choice. Find out more about all the Roald Dahl characters in a special post that we posted for Roald Dahl Day.
Join Harry, Hermione and Ron on a magical adventure as the three budding wizards progress through Hogwarts School of magic. J.K.Rowling is famous for her detailed narrative enabling children to build up the character using their own imagination. Transform into a wizard and follow in the footsteps of Harry this year for World Book Day in licensed Harry Potter Costumes.
Where's Wally was created nearly 30 years ago by British illustrator Martin Handford, and he is now a national icon. The character appears in over 7 books wearing his famous red and white top and blue trousers. Wally has since been joined by Wilma, Wenda, Odlaw and Woof, Wallys dog in countless illustrated adventures. This is a great option for kids who want to stand out at school and think of the fun you can have playing hide and seek in the school playground.
Prepare to embark on an adventure into the deep, dark woods with your forest friends under the disguise that Grufallo is watching over you. The Grufallo, half grizzly Bear and half Buffalo created by Julia Donaldson is a popular choice for nursery and pre school children. The Travis Designs Monster Cape can be worn over your school clothes and doubles up as a rather impressive Gruffalo costume.
Horrible Histories books engage children in historical events through gruesome stories told in a tongue-in-cheek manner which makes history fun. The illustrated books have created a number of horrific and disgusting historical characters including Queens, Kings, Peasants, Tudors, Victorians, Knights, Vikings and the infamous Guy Fawkes. Choose your favorite historical character for World Book Day.
Winnie The Pooh
AA Milne's Winnie The Pooh, is a childhood classic for many and is the perfect choice for younger children at nursery and pre-school. Pooh Bear, Eeyore, Tigger and Piglet are some of the most celebrated book characters. Shop the quality Travis Designs range of Disney Baby costumes for your little one.
Treasure Island and Pirates of the Caribbean have made pirates a popular choice for World Book Day. Set sail on the high seas and celebrate literature at the same time as perfecting your pirate slang. There are many different options for this theme from a Jack Sparrow costume to a pirate kit including a sword, eye patch and bandanna. Ahoy there matey!!
Transform into a fairy-tale character on World Book Day including Little Red Riding Hood, Cinderella, Alice in Wonderland, The Red Queen or Robin Hood. Dressing up as a classic fairy-tale characters will enable your children to take part in fanciful adventures that differ from modern classics.
The Tiger Who Came to Tea, Jungle Book and more recently Dinosaurs love Underpants has made dressing up as animal a further option for World Book Day. Check out our fun range of tiger and dinosaur onesies and our other animal costumes that are perfect for cheeky little monkeys.
Click HERE to enter the competition to Win a World Book Day Costume.


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Despite it's spring on the calendar, it's summer for Katie Holmes.
The star decided to honour the Easter weekend at the Marco Island Beach Resort (Florida). She took Suri Cruise and her family with her.
Everybody flew from Ohio and the actress and her daughter met them for a week-long stay. The family was enjoying beach days and ice-cream.
Holmes shared few mother-daughter snaps on the Web. One time Katie rented a jet ski. Suri was super-excited to go fast over the waves. Holmes gave all cousins a turn.
An insider tells that Katie is a fun aunt and is really close with her nephews and nieces.


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Pure HCG Diet: Metabolic Assessment Form and Notes
"Negative results are just what I want. They're just as valuable to me as positive results. I can never find the thing that does the job best until I find the ones that don't."
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If you didn't purchase a qualifying plan to get the private consult with Dr. Kepo'o, simply download it, fill it out, and after you have lost the weight, fill it out again and compare it to the original. You may be surprised to see just how much your health has improved, not just in pounds lost, but in areas you hadn't even thought of.
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First Ever Apple PC
Apple is known nowadays for their macbooks, iphones, ipods, ipods, apple computers, etc. Before all that though apple had a computer that they called the Apple I. This computer was …Continue reading
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Want to stay "green" while keeping your garden green? Now you can with the solar-powered Electric Lawnmower. Over the course of our adapting to greener measures, we have certainly opened …Continue reading
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There are few these days who can spend without worrying about the amount. Even high end consumers are being a lot more frugal these days in the midst of all …Continue reading
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San Fransisco based startup Regen has introduced a new iPod dock that will surely get environmentalists excited. The Reverb uses sunlight to generate power with its integrated photovoltaic panel. If …Continue reading
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Premium electronics manufacturer Bang & Olufsen doesn't skimp on components. Perhaps that's why audio/video enthusiasts continue to rave about their products decade after decade. The Bang & Olufsen BeoVision 7-40 …Continue reading
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So what's so special about a sound system? First of all, this system isn't for your home, it's for your car. To be more exact, it's for your 2010 Porsche …Continue reading
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By manipulating the design of an item used everyday into a sensual, feminine form we have created a personal gesture for the urban lifestyle of the working woman. The design …Continue reading
Vertu Signature S
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text: BANGKOK, Nov 27 (Reuters) - Twenty Burmese dissidents are due to face trial in Thailand for illegal entry, police in the western province of Kanchanaburi said on Thursday. The Burmese, who say they are members of various opposition groups in their homeland, were arrested on Monday and were detained at Sangkraburi police station, police said. ``The court is set to rule on them tomorrow. They may be fined or face one month jail before deportation,'' police in Sangkraburi on the border with Burma told Reuters by telephone. Illegal entry carries a 3,000-baht ($76) fine or a one-month jail term. In Bangkok a Burmese dissident student group, the All Burmese Students Democracy Front (ABSDF), issued a statement denouncing the arrest of its members, demanding their release, and urging the government to stop suppressing Burmese opposition members living in Thailand. ``We denounce the arresting (of) Burmese dissidents, including members of NLDLA (National League for Democracy Liberated Area), ABSDF, and KNU (Karen Nation Union) on November 23,'' the group said in a statement obtained by Reuters. It also asked the government to ``remove the suppressing policies toward Burmese opposition in Thailand.'' Thai police said they had only arrested people for entering the country illegally. Thousands of Burmese students fled from military suppression after the Rangoon government crushed uprisings in September 1988, several hundred joining the ABSDF or the KNU rebels in the jungles on the Thai-Burma border. ^REUTERS@


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This from my brother. Forwarded without comment.
-----Original Message-----
F(Name snipped)
Sent: Thursday, May 16, 2002 11:26 AM
To: firstname.lastname@example.com
Subject: Re: FW: What does a liberal think?
Thanks Shu, I sometimes wonder why people make such a big deal about
something that people have no control over. Seems to me there are much
more pressing issues in the world. Besides, the TEN commandments which
are the ONLY things in the bible etched in stone refer to adultery NOT
homosexuality. Seems to me people in the church never reference a good
chunk of folks in church who have been, or currently are in an adulterous
situation. Maybe conservative folks need to focus their energy on hunger,
war, famine, poverty etc than whom one finds to love in this world.
Especially after 9/11, these conservative folks need to BACK OFF and
think about more important things, Okay I have ranted enough, hope all is
well, lets talk later this week so we can catch up, okay, Love, G. Oh, if
there are any such folks you want to forward this ranting to, don't
hesitate to do so. Another book that addresses this and other issues and
is a really good read is THE GOOD BOOK by Peter Gomes.
This archive was generated by hypermail 2b29 : Thu May 16 2002 - 12:46:02 EDT


text:
Mobile phones
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Begin by communicating your mobile telecoms needs to Coltel and we will help you to develop the best mobile communications package for your company.


text:
- In a large pot bring the soup stock and peas to a boil. Then reduce the heat to low and simmer for 45 minutes.
- Add the remaining ingredients and cook on low for another 15-20 minutes (until the carrots & peas are cooked).
- Add more water if you prefer a thinner soup.... I like it thick & hearty!
Homestyle Split Pea Soup (for my poor sweet husband who is sick with the flu)
17 comments:
sounds delish- I hope your charge feels better soon!ReplyDelete
this sounds delicious!!! Good jobReplyDelete
I just may have to try this for my new recipe of the week next week.. it looks soo good! I am a huge fan of soup :) Hope he feels better!ReplyDelete
YUM! its so cold here, i need this! love the blog! cant wait to start following and catching up with your posts! Love it!ReplyDelete
I hope he feels better soon. my man is sick, too, so I might give your soup a try. sounds and looks delicious :)ReplyDelete
Oh so sorry that your husband is having the flu...Poor him. I hope he will feel better soon and that soup looks so delicious. You know that I love soups. Im going to try this one over the weekend:)Yum! Happy Tuesday, darlingReplyDelete
hahaha men and illness are always hilarious. Reminds me of this video:ReplyDelete
the soup looks delicious!!!
Thea, that video is HILARIOUS!!! Oh the man cold....ReplyDelete
: )
Ohh I hope he gets better. Half my office was out with the flu. Soup and tea!! =)ReplyDelete
Melanie's Randomness
Hope your sweetie is feeling better soon! You certainly are taking good care of him :) This soup looks super yummy - I love that you added dill, I'll have to try that with split peas soon!ReplyDelete
Oh you're so sweet for making him homemade soup :)ReplyDelete
aw, poor guy!!ReplyDelete
Hope he's feeling better. That soup looks delicious!ReplyDelete
Looks delicious sweetie!ReplyDelete
Was just chatting with my mom on skype and she told me that she tried your recipe and LOVED it! She said and I quote "I could eat it every day". Pretty impressive:)ReplyDelete
Bri, that's so cool that your mom made the soup! I'm glad she liked it. It really is a simple & reliable recipe : )ReplyDelete
This looks WAY better than any split pea soup I've ever seen! And the perfect soup season :)ReplyDelete


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Father D'Arcy reveals cancer fight
By staff reporter
THE Enniskillen-based priest Fr D'Arcy has revealed he is fighting prostate cancer.
One of Ireland's best known priests, also a popular columnist and broadcaster, the 69-year-old said he is feeling 'fine' as he battles to overcome the disease, which he was diagnosed with earlier this year.
"I don't really like saying too much about it," Fr D'Arcy told the Belfast Telegraph. "It's prostate cancer, which any man can get at any stage. I haven't said much about it because it's putting yourself in the limelight, but thankfully I'm overcoming it."
Fr D'Arcy, speaking during an in-depth interview with the paper, spoke of being formally censured by the Congregation for the Doctrine of the Faith in April 2012, which wrote to the Superior General of his religious order, the Passionists, accusing him of being "a source of great scandal".
He said the stress of that time may not have caused the cancer, but certainly didn't do his health any good: "In the end I said to myself, am I going to let a crowd in Rome destroy my health?"
He said he was doing well and had not let the cancer get in the way of his work. He said: "I'm old enough to realise that when you think you're fine there is probably something wrong.
"Not many people have been aware of the fact I've cancer, I never hid the fact, but very few people have referred to it."
Fr D'Arcy entered the priesthood at aged 17 and is Rector of St Gabriel's Retreat in the Graan, near Enniskillen. He has penned a popular column for the Sunday World for almost 40 years.


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Output rose by 7.6% in July compared to the same month last year, to 126,566, amid double digit growth for home and export markets, the Society of Motor Manufacturers and Traders (SMMT) said.
The number of cars built totalled 1,023,723 in the first seven months of the year, the first time the one million milestone was reached in July since 2004.
Exports were up by 6% last month, with a 14% rise in output for the domestic market.
More than three-quarters of a million cars built in the UK this year were for overseas markets - almost four out of five of all cars manufactured.
Mike Hawes, SMMT chief executive, said: "UK car production in 2016 is booming, with new British-built models in demand across the world.
"Manufacturers have invested billions to develop exciting new models and produce them competitively here in the UK.
"Future success will depend on continued new car demand and attracting the next wave of investment so Britain must demonstrate it remains competitive and open for business."
Business and energy secretary Greg Clark said: "Our automotive sector continues to go from strength to strength thanks to our highly-skilled workforce and long-term investment in new technology and innovation.
"This government's comprehensive industrial strategy will build on that success, ensuring that we have the right infrastructure, skills and support in place for our world-leading industries as well as support for new emerging sectors to flourish."
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text:
Division 3
West Wittering 129
Crawley Nayee 132-4
Crawley Nayee bowler Sachin Parekh orchestrated a brilliant six-wicket victory over West Wittering at Bewbush Pavilion.
Sachin took five for 39 off his 14 overs to bowl out West Wittering for 129 in 39.2 overs.
He was well supported by Jay S Nayee who took three for 52 and some excellent close catches by the fielding unit.
Crawley Nayee lost Rakesh Parekh early when the score was at 12 for 1 in 3.5 overs.
Sunil Bhatt and Nikulkumar Patel went about chasing the 129 briskly before losing Sunil Bhatt (36) at 70 for 2 in only the 13th over.
Richard Hammond struck with further two quick wickets which saw the back of Nikulkumar Patel (36) to slow the Nayee's run-rate. Again it was Sachin Parekh (33 not out) who came to the rescue putting on a 51-run partnership with Raj Nehru to see Nayee to their second victory of the season.
Don't miss out on all the latest breaking news where you live.
Here are four ways you can be sure you'll be amongst the first to know what's going on.
1 Make this website your homepage
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4 Register with us by clicking on 'sign in' (top right corner). You can then receive our daily newsletter AND add your point of view to stories that you read here.
And do share with your family and friends - so they don't miss out!


text:
Kathleen Culligan Techler
For heartwarming romances choose these three anthologies by Kathleen Culligan Techler, Leta Nolan
Childers, Linda Bleser, and Elizabeth Delisi.
For more information, click here.
Perfect for Christmas giving or reading:
Linda Bleser's HOME FOR CHRISTMAS,
Leta Nolan Childers' MISTLETOE IN THE MOUNTAINS,
Elizabeth Delisi's A CAROL OF LOVE, and
Kathleen Culligan Techler's A CHRISTMAS FOR CAROL
Back by popular request: More stories by the authors of HOLIDAY HEARTS.
IMPERFECT CHRISTMAS by Linda Bleser
HOLIDAY HEAVEN OR HELL by Leta Nolan Childers
A CUP OF CHRISTMAS CHARM by Elizabeth Delisi
THE BAFFLING HOLIDAY MYSTERY by Kathleen Culligan Techler
SECRET ADMIRER by Linda Bleser
CUPID CRASHES THE PARTY by Leta Nolan Childers
THE HEART OF THE MATTER by Elizabeth Delisi
A BIT OF DECEPTION by Kathleen Culligan Techler
It's never too early or too late for Valentine's
Day reading.
For more information or to order click here.
Enter supporting content here


text:
Kakavia (Aegean Fish Stew)
"Greek fishermen make this out at sea, using whatever they've hauled into their boat that day, and cooking it in seawater… because their water is ready-salted they don't need any seasoning at all to achieve a perfectly delicious stew. Genius!"
– Jamie Oliver, 'Jamie Does'
– Jamie Oliver, 'Jamie Does'
More Photographs inside.
Things weren't boding well for 'K'. We'd had a manic weekd – I didn't leave the office before midnight the whole week – a hectic weekend with much socialising, and I hadn't the faintest idea what to make for 'K'. So the last thing I felt like doing on a Sunday evening after a boozier than usual Saturday was cooking for the project. I really shouldn't have put it off, because this week has been only marginally better than the last.
Chopped up
I managed to get home by 8p.m. on Wednesday and grumblingly set about making this dish from one of my favourite Jamie Oliver recipe books. By the time we prepared the dish, took the photos and settled down to eat, it was 9:30 and I must admit, I was NOT in the best of moods. Cooking is my therapy, it is true, but there are times when chopping vegetables just doesn't have its usual calming influence. Those times are rare but this was one of them. But this lovely stew changed all of that and made everything right with the world. It was wholesome; it was comforting; it was just what I needed after a spate of frenetic days in the office and a crazy weekend.
Rustic Bread
I could eat bowls of this quite happily for the whole week and keep to my resolution of eating healthily with no trouble whatsoever.
Kakavia (Aegean Fish Stew)


text:
Working together for Palestine Saturday 9th July, 10am-5pm, University of London Union, Malet Street, London WC1E 7HY - Palestine Solidarity Campaign. "We are the people and this is our time: stand up, sing out … for Palestine"
Join Dave Randall, from Faithless & the Freedom for Palestine single, Dr Karma Nabulsi, Hugh Lanning, Andy Slaughter MP and many others at our Working Together for Palestine Conference.
Representatives from a wide range of organisations will join with activists to share ideas and plans for the year ahead. Make sure your organisation is represented! This conference follows our very successful conference in July 2010, and our follow-up meeting this March with other groups.
Solidarity with Palestine is now recognised as the key international issue, and the movement in Britain is playing a vital role. PSC is organising this conference in order to strengthen our common links and 'jump together' when possible to increase the impact of the campaigning work that we are carrying out.
We look forward to seeing you at the conference on Saturday 9th July. Registration is essential:
Register now!
Please register for the event by emailing Martial Kurtz at email@example.com with the following information
name of organisation you are representing:
Mobile number:
"We'll break down the walls, demand justice for all,
Freedom for Palestine"
by One World – Watch the video, Share the info and Buy the single for only 79 pence here:
UNISONActive is an unofficial blog produced by UNISON activists for UNISON activists. Bringing news, briefings and events from a progressive left perspective.


text:
Currently painting up 9 Dunedain for my latest project!
I have recently been building up my collection of Lord of the Rings figures and sourcebooks. My plan is to play though the 3 campaigns matching the books/films, painting the figures and making the terrain as I go. This should be an interesting project to undertake and also make for an interesting solo gaming experience, with an interesting narrative replacing repetative one-off battles.
Scenario 1: The Hunt Begins, takes place after Sauron has discovered from Gollum that the One Ring is in the possesion of a creature called 'Baggins' in a land called 'Shire'. He has dispatched the Ringwraiths, who have found out where the Shire is and are now approaching it's borders. But the Shire is not unprotected. The Rangers of Arnor have long protected this peaceful land and will sell their lives to prevent the Nazgul from crossing into the Shire.
The scenario sees 9 Dunedain (plus reinforcements as the game goes on) trying to prevent 3 Ringwraiths from getting two of their number off the board and into the Shire. Being this far from Sauron's influence, the Ringwraiths are relatively weak, but 3 of them are still a formidable force.
When the figures are all ready I hope to post a full report on the battle.


text:
ISN offers a variety of meetings and events throughout the year to provide in person account assistance at no additional cost. Please find brief meeting descriptions below:
Users Group Meetings provide an overview of ISNetworld, demonstrate existing functionality and allow customers to provide feedback on topics discussed. UGMs are a way for contractors to learn about the different marketing opportunities and benefits available through ISNetworld. These meetings serve as an opportunity for contractors to meet current and prospective Hiring Clients. Help Desks are traditionally scheduled in conjunction with Users Group Meetings. Help Desks are 30 minute appointments where customers receive one-on-one assistance with their ISNetworld accounts.
Hiring Client Roundtables are designed to keep current and prospective Hiring Clients up-to-date on ISN's products and services. These meetings serve as a forum for industry peers to network and to share best practices, as well as to find out how other companies use ISNetworld.
Regional Workshops are one-day meetings offered where customers receive demonstrations on additional functionality available within ISNetworld. Workshops include presentations from ISN Hiring Clients, contractor breakout sessions and one-on-one assistance from an ISN representative.
The ISN Users Conference is a two-day event that brings together Hiring Clients, contractors and suppliers from around the globe. The Users Conferences provides attendees an opportunity to hear best practices from industry leaders, to learn how to maximize their ISNetworld subscriptions and to network with other users.


text:
Includes remixes from Clams Casino, Hudson Mohawke and more.
Endtroducing is 20 years old and to celebrate this milestone DJ Shadow has re-opened the sessions to personally curate a bonus album of remixes by a carefully selected roll call of electronic music luminaries, which he has christened Endtroducing Re-Emagined. This will come with expanded notes from a new interview conducted by Eliot Wilder and never-before-published photographs to create the 20th anniversary release as Deluxe 6LP.
1.Best Foot Forward,
2.Building Steam With A Grain Of Salt,
3.The Number Song,
4.Changeling / Transmission,
1.What Does Your Soul Look Like [Pt. 4],
3.Stem / Long Stem / Transmission 2,
1.Mutual Slump,
2.Organ Donor,
3.Why Hip-Hop Sucks In '96 4.Midnight In A Perfect World,
1.Napalm Brain / Scatter Brain,
2.What Does Your Soul Look Like Pt. 1 / Blue Sky Revisit / Transmission 3,
1.Best Foot Forward,
2.Building Steam With A Grain Of Salt,
3.The Number Song,
4.Changeling [Demo Excerpt],
3.Red Bus Needs To Leave!


text:
The first step in getting started with Riot Athletics is either to come in and take a class with us, or if you are unsure on where you want to start, you can set up a time to tour the gym and meet with a coach to discuss your training goals. This free consultation not only allows us to give you a proper welcome to our community (we love meeting new people!), it also ensures we set you up for success by identifying any possible limitations or past stumbling blocks. Whether you are looking for a personal trainer or interested in a group training setting, we can get you started down the right path.
You can also check out our SERVICES page for more information on membership options.


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Dad's Army
'Allo 'Allo
Are You Being Served?
It Ain't Half Hot Mum
They were all British comedy classics from the pen of comic genius, David Croft.
I caught a re-run of an old British comedy classic, 'Allo 'Allo, the other day on TV and it reminded me of just how funny and clever some of these old shows were. It was also perfect timing in that it was only a couple of weeks ago that I published my Why all the Bloody Swearing article about the amount of unnecessary swearing that there seems to be on British television today.
It's rare that I actually find myself giggling out loud to myself, but 'Allo 'Allo, which was a British sitcom set in Nazi Occupied France during the Second World War, had me doing just this. The show creates much of the humour by the representation of the different nationalities of the various different characters through their speaking of their lines in English, but with stereotypical national accents. Along with the accents, came the innuendos of the mispronounced English words.
In the show, the character of Crabtree, an undercover English Agent disguised as a French speaking Gendarme, would mispronounce his vowels in lines such as:
"I was pissing by the door, and I thought I would drip in".
The show was broadcast at peak time viewing on the BBC between 1982 and 1992 and an excellent example of how you can swear without swearing. It was written and created by David Croft and Jeremy Lloyd, who also co-wrote other classic shows including Are You Being Served?
David Croft, along with co-writers Jeremy Lloyd and Jimmy Perry were responsible for many of the British comedy classics from the 1960's right up until his death in September 2011.
David Croft
David Croft was born 7 September 1922 into a showbiz family. His mother was a famous stage actress; Annie Croft and his father Reginald Sharland a radio actor. In World War Two, Croft served in North Africa, India and Singapore and rose to the rank of Major.
When he had finished his military service he went into a career in entertainment. Following a period acting and TV production, he met Jimmy Perry who gave Croft a script for a pilot TV show called The Fighting Tigers about the British Home Guard during the Second World War. The show, re-named, became the classic Dad's Army, which Perry and Croft went on to co-write between 1968 and 1977.
While Dad's Army was still running, Croft began to Write Are You Being Served with Jeremy Lloyd, another show that became a long running classic. He continued writing in partnership with both Perry and Lloyd for many years and between them they created some of the best loved British TV comedy shows of all time.
David Croft died in his sleep at his home in Portugal on 27 April 2011, aged 89.
"Don't tell him Pike"
Written by David Croft and Jimmy Perry, Dad's Army was a sitcom about the British Home Guard during the Second World War which ran from 1968 to 1977.
The Home Guard was a military force intended to be the last defence against German invasion of Britain and consisted of those not eligible for military services due to age, reserved profession or ill health.
The show depicts the antics of, the generally elderly, members of the Walmington-on-Sea Home Guard as they bumble their way through the war preparing to fend off the impending invasion often armed with no more than broomsticks and wooden guns.
Though the inept soldiers even have difficulty fending off their own local Air Raid Precautions (ARP) warden let alone the Nazi Hordes, the show still managed to present a respectful homage to those who were left at home, but who were still ready to fight.
"Listen very carefully; I shall say 'zis only once!"
'Allo 'Allo!, co-written by David Croft and Jeremy Lloyd was set in the Second World War, but this time in a small village in occupied France. Written as a parody of the TV drama series Secret Army, the plot revolves around Rene, who owns and runs a small café, and his attempts to return home English airmen stranded in France whilst at the same time keeping some valuable artefacts that the local German Commandant has forced Rene to keep for him hidden from the Gestapo. Artefacts which include the painting: The Fallen Madonna with the Big Boobies'.
All of the characters of various nationalities, including English, French, German and Italian speak their lines in English, but in exaggerated stereotypical accents which leads to many bad pronunciations of words which in turn lead to many innuendos. If it were not for the fact that the upper class English airmen were lampooned as well, the stereotyping might be considered offensive today but instead are still hilarious.
"The least sign of danger and my pussy's hair stands on end"
More stereotyping, but this time sexual rather than national with the ambiguous, never quite outwardly gay, character of 'I'm free' Mr. Humphreys played by John Inman.
A further collaboration with Jeremy Lloyd, the show, which was set in a department store, ran for 69 episodes between 1972 and 1985.
Nearly all of the episodes were set inside the men's and ladies-wear department of the store, Grace Brothers and parodied beautifully the British class system of the time.
The show that had a name that became a national catchphrase. 'Hi-de-Hi' was the cry that greeted the happy campers from loudspeakers situated around the post war holiday camp, Maplins, where the series was set.
For those who are not British, or those never had the pleasure, a holiday camp was a place where people, particularly in post war Britain, could get a cheap holiday, entertainment included, for the equivalent of one week's pay. To give you an idea of the type of holiday, such camps were used as military camps during the war.
Set in a Joe Maplins camp, the show revolves around the antics of the staff of the camp known as Yellow Coats owing to their yellow blazers that they wore as a uniform and which was a parody of the real life Red Coats of Butlin's holiday camp fame.
A mixture of pure slapstick and comical observation of the British class system, I have to be honest and say that it was not really one of my favourites but I've included it here because of its popularity.
Written again by David Croft and Jimmy Perry, the show ran from 1980 to 1988 and attracted large audiences and its catchphrase and its characters, particularly the welsh Yellow Coat, Gladys Pugh who was for ever fawning over the upper class entertainment manager of the camp.
"Shut up, you 'orrible little man!".
Of all the classic comedy's that David Croft wrote, the one that will rarely, if ever again, be repeated on British TV is It Ain't Half Hot Mum.
Co-written with Jimmy Perry, It Ain't Half Hot Mum was broadcast between 1974 and 1981 and was set in the later period of the Second World War following the German surrender but before the defeat of the Japanese in Asia.
The show revolved around a concert party providing entertainment for the troops that were being stationed in India before being sent to the front lines.
The reason that this show in particular caused controversy was its use of a white actor, Michael Bates, playing an Indian role in the form of the character Rangi Ram. A move that led to accusations of the use of 'blackface' actors as the practice was called.
Jimmy Perry refuted this claim in an interview in 2013 by claiming that Michael Bates wore only a light tan makeup!
British Comedy Classics
Whatever your views are on the rights and wrongs of stereotyping people for comedic purposes, these shows were right for their time and never written to be offensive.
Put aside your more modern sensitivities for a while and just listen to the quality of the writing. The set up and commitment, as a salesman would call it, is brilliant and all delivered by some of the best comedy actors of the era.
These shows didn't become comedy classics for nothing.
You may also like:


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Indiana Farmers Coliseum Information and Schedule
Looking for tickets to the upcoming game or show at Indiana Farmers Coliseum? Well then you've come to the right place. At InSeats.com we carry ticket to every event at Indiana Farmers Coliseum. We know that Indiana Farmers Coliseum tickets can sometimes be tough to get, but at InSeats.com we make the ticket buying experience as simple and reliable as possible.


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Diane Kruger (born Diane Heidkrüger, 15 July 1976) is a German-American supermodel-turned-actress known for her roles in National Treasure, Inglourious Basterds, and Troy; two seasons as the co-lead for The Bridge; and for being Joshua Jackson's girlfriend.
- Duelles (2002): Sabine
- The Piano Player (2002): Erika
- Troy (2004): Helen
- Wicker Park (2004): Lisa
- National Treasure (2004): Abigail Chase
- Joyeux Noël (2005): Anna Sorenson
- Copying Beethoven (2006): Anna Holtz
- National Treasure 2: Book of Secrets (2007): Abigail Chase
- Anything For Her (2008): Lisa
- Inglourious Basterds (2009): Bridget von Hammersmark
- Fringe episode "Olivia. In the Lab. With the Revolver" (2010): Miranda Green.
- Mr. Nobody (2010): Anna
- Unknown (2011): Gina
- Farewell My Queen (2012): Marie Antoinette
- The Host (2013): The Seeker/Lacey
- The Bridge (US) (2013-2014): Sonya
She provides examples of:
- Bilingual Bonus: In addition to her mother tongue (German), she speaks French and English fluently and can dub herself in three languages with no accent. She got to show it off in Inglourious Basterds. Apparently, her English was so good that Quentin Tarantino nearly turned her down for Inglourious Basterds because he wanted an "authentically" German actress.
- The Chick: About 95% of her English-speaking roles. Fittingly the role that first gave her widespread exposure to English language audiences was Helen of Troy, the original So Beautiful, It's a Curse sufferer.
- Pimped-Out Dress: Nearly always. Being Karl Lagerfeld's muse has its perks.
- Pretty in Mink: Has shown up places wearing a few furs, like a brown rabbit bolero jacket.
- So Beautiful, It's a Curse: Speaking of which, she's something of an example herself, assuming that Typecasting is a curse (and for an actor with serious talent, it is). She looks so perfectly like a classically gorgeous Hollywood Actress that it's difficult for her to be cast credibly as anything else. Tellingly, her two most well-known (serious) English roles for many years were as the aforementioned most beautiful woman in the world and as a professional (and famous) actress in Inglourious Basterds.
- Unlimited Wardrobe


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Stage Lighting Package Kit 2
Order code: TA-SETI2
6628.30 je Pack .
incl. VAT plus Shipping cost
Complete stage light equipment set for school hall or university.
Manufacturer: GEO-Technik
Manufacturer: GEO-Technik
High-quality system with 10 lights, control and all necessary accessories.
The installation must be performed by a specialist.
2 load bar for hanging the lights 300cm.
4 folding hook with eyelet + 4 steel cables for hanging up to 500cm.
8 lens spotlights 1000W + halogen lamps
8 color filter + color filter frame
8 folding hook + 4mm safety rope
2 Wall Brackets Folding
2 profile spots 1000W + halogen lamps + safety rope
1 lighting console Aurora 12 + PSU
2 Wall Dimmers, each of 4 x 2300W
1 DMX control cable 10m assembled
1 DMX control cable 40 m
2 wall plug points for selectively connecting the control console.


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As some people may know, Iain was the Technical "goto" guy in the UK for Kaleidescape for nearly 3 years, before he decided to re-start Kustomworks Ltd again, due to such high demand from our clients we have decided to become Kaleidescape dealers to strengthen the support we can give our clients.
Kaleidescape is in a class of its own, been the only product that provides and "Experience" like no other.
If movies are your passion then you haven't watched a movie until you have watched it using a Kaleidescape.
Imagine all of your favourite movies at your fingertips. Imagine breath-taking audio and video fidelity. Imagine the lights dimming automatically and your movie playing instantly without the distraction of previews, menus or ads.
You are just beginning to imagine the Kaleidescape Experience.
We are well versed with the Kaleidescape product, and we have pushed the boundaries of the Kaleidescape product for the last 7 years, we have installed countless systems in varied locations all over the globe, you can rest assured that Kustomworks have the technical expertise to install, program and support Kaleidescape installations in any location.
If you would like more information, or a demonstration of the Kaleidescape system, please get in touch and one of our sales people will be in touch with you.