text:
- Research
- Open Access
Proteomic analysis of the cerebrospinal fluid of patients with restless legs syndrome/Willis-Ekbom disease
© Patton et al.; licensee BioMed Central Ltd. 2013
- Received: 14 December 2012
- Accepted: 8 May 2013
- Published: 7 June 2013
Restless Legs Syndrome/Willis-Ekbom Disease (RLS/WED) is a sensorimotor disorder that causes patients to experience overwhelming and distressing sensations in the legs compelling the patient to move their legs to provide relief. The purpose of this study was to determine if biomarkers in the cerebrospinal fluid can distinguish RLS/WED patients from neurological controls.
We obtained CSF samples by lumbar puncture from 5 early-onset RLS/WED patients and 5 controls. We performed 2-dimensional difference in-gel electrophoresis (2D-DIGE). Proteins that were significantly altered were identified by Student's t-test. Protein spots that were differentially expressed (p ≤ 0.05, Av. Ratio ≥ 2.0) between RLS/WED and control CSF samples were identified using MALDI-TOF-MS. Statistical analyses of the validation immunoblot assays were performed using Student's t-test.
In this discovery study we identified 6 candidate CSF protein markers for early-onset RLS/WED. Four proteins (Cystatin C, Lipocalin-type Prostaglandin D2 Synthase, Vitamin D binding Protein, and β-Hemoglobin) were increased and 2 proteins (Apolipoprotein A1 and α-1-acid Glycoprotein) were decreased in RLS/WED patients.
Our results reveal a protein profile in the RLS/WED CSF that is consistent with clinical findings of disruptive sleep, cardiovascular dysfunction and painful symptoms. Moreover, protein profiles are consistent with neuropathological findings of activation of hypoxia inducible factor (HIF) pathways and alterations in dopaminergic systems. These data indicate the CSF of RLS/WED patients may provide information relevant to biological basis for RLS/WED, treatment strategies and potential new treatment targets.
- Sleep disorders
- Restless legs syndrome/Willis-Ekbom disease
- Nitric oxide
- Hypoxia inducible factor
- Pain
Restless Legs Syndrome/Willis-Ekbom Disease (RLS/WED) is a sensorimotor disorder that affects between 5-10% of the population [1]. Patients who suffer with RLS/WED experience an overwhelming and distressing sensation that forces them to move their legs [2]. Those with moderate to severe symptoms report significant disability, chronically reduced sleep times, 20% reduction in their work productivity, diminished quality of life, and increased depression and anxiety [3]. RLS/WED has also been identified as a risk factor for cardiovascular disease [4–7]. RLS/WED prevalence increases with age and it affects women twice as frequently as men.
Iron dysregulation in the pathogenesis of RLS/WED is supported by a substantial volume of research including several studies reporting decreased ferritin in cerebrospinal fluid (CSF) and decreased brain iron particularly in the substantia nigra using MRI, ultrasound imaging and brain autopsy analysis [8]. A number of studies to date support the concept of diminished brain iron in RLS/WED, thus providing the basis for the hypothesis that RLS/WED occurs as a result of low brain iron content [8–10]. We still do not understand, however, the full consequences that low brain iron may have on neural systems in patients with RLS/WED. We performed proteomic analyses in order identify neural systems and pathways that are altered in those suffering with RLS/WED and possibly identify novel avenues for potential therapeutic intervention.
CSF was utilized for this biomarker study because it is most likely to reflect changes in CNS metabolic status due to its proximity to the brain. An advantage of CSF as the biological fluid for proteomic analyses over blood is that the CSF is sequestered behind both the blood–brain and brain-CSF barriers. This isolation permits the identification of biomarkers that are specific to CNS disease processes. Hence, our goal for this study was to identify CSF biomarkers for RLS/WED using two-dimensional difference-in-gel electrophoresis (2D-DIGE) together with tandem Mass Spectrometric (MS) analysis. To further validate the identified biomarkers, we used immunoblot analyses to quantify differences in protein levels between early-onset RLS/WED and age- and gender-matched control subjects. In this study, we identify six proteins whose levels are altered in early-onset RLS/WED subjects.
Patient demographics and CSF collection
RLS/WED patient demographics
Subject number
Onset age (years)
Family history
Sleep apnea or snoring
Ages of RLS/WED and control subjects at time of study
Subject number
RLS/WED (age in years)
Control (age in years)
Mean ± SD
Mean ± SD
58.00 ± 12.10
58.80 ± 7.66
After written informed consent was obtained from the research subjects, approximately 10 mL of CSF was obtained via lumbar puncture with a 25-gauge needle and placed immediately on ice. CSF specimens were spun at low speed in a refrigerated centrifuge to remove any cellular contamination, aliquoted, and stored at −80°C within 1 h. All samples were collected between 9:30 AM and 11:00 AM. The CSF samples were precipitated with trichloroacetic acid/acetone following storage at− 80°C and the protein pellets were resuspended in 10 μL of DIGE labeling buffer (7 M urea, 2 M thiourea, 4% 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) and 30 mM Tris/HCl, pH 8.5). Protein levels were quantified using the 2D Quant-Kit (GE Healthcare, Uppsala, Sweden).
Isoelectric pH gradient (IPG) strips, urea, 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS), dithiothreitol (DTT), Pharmalyte pH 3–11, acrylamide, ammonium persulfate, TEMED, glycine, methylene bisacrylamide, TRIS, thiourea, and 2-D Quant Kit (PlusOne or Ettan™ sample preparation kits and reagents) were obtained from GE Healthcare (Piscataway, NJ, USA). ReadyPrep 2D-Clean-up Kit was obtained from Bio-Rad (Hercules, CA, USA). Iodoacetamide and mineral oil were obtained from Sigma (St. Louis, MO). SDS was obtained from USB (Cleveland, OH, USA). Methanol and glacial acetic acid were purchased from Fisher Scientific (Fair Lawn, NJ, USA).
Two-dimensional difference-in-gel electrophoresis (2-D DIGE) labeling
Each of the dry CyDyes (GE Healthcare) was reconstituted and 50 μg of total protein was labeled with each diluted CyDye solution as described in the manufacturer's protocol.
2-D PAGE analysis
CSF containing 150 μg of labeled protein was added to an IPG strip. Strips were rehydrated for 20 h followed by isoelectric focusing for 27 h. The following gradient program was used – step 1: 0.01 h, 150 V, 2 mA, 5 W; step 2: 2.00 h, 150 V, 2 mA, 5 W; step 3: 6.00 h, 500 V, 2 mA, 5 W; step 4: 5.00 h, 3500 V, 2 mA, 5 W; step 5: 9.20 h, 3500 V, 2 mA, 5 W; step 6: 5.00 h, 50 V, 2 mA, 5 W. SDS-PAGE was carried out in 12.5% of acrylamide. Following the focusing step, the proteins on the strip were reduced and alkylated with gentle shaking in two subsequent steps for 30 min each in 10 ml equilibration buffer (6 M Urea, 75 mM Tris–HCl pH 8.8, 29.3% glycerol, 2% SDS, 0.002% bromophenol blue) containing 100 mg DTT and 250 mg iodoacetamide in the second step. Strips were removed from equilibration solution, blotted, and placed on a 1-mm thick 25.5 × 20.5 cm 12.5% acrylamide non-gradient SDS gel. The strips were sealed with 1% agarose, 125 mM Tris–HCl at pH=6.8, 1% SDS. Proteins were separated on the gels using 600 V, 400 mA, 100 W for 4 h at 10°C. Analytical gels were imaged immediately after separation, while the preparative gels were fixed with 7.5% acetic acid/10% methanol overnight and stained with Coomassie Blue R-350. The images from analytical gels were collected as described previously [13]. Intra-gel spot detection, inter-gel matching and normalization of spot intensities were performed using the Differential In-gel Analysis (DIA) mode and Biological Variation Analysis (BVA) mode of DeCyder 2D software (GE Healthcare, Munich, Germany), respectively. In-Gel Trypsin Digestion of Proteins and Mass Spectroscopic analyses for these experiments were performed as described previously [13].
Immunoblotting analysis
Each of the proteins identified by 2-D DIGE analyses was confirmed by immunoblot analyses. Protein concentration was determined and immunoblot analyses were performed as described previously [14]. An aliquot of total CSF containing 1-3 μg from each of the subject participants was used for the validation analyses. Mouse monoclonal anti-apolipoprotein-A1, anti- α-1-acid glycoprotein, anti-vitamin D binding protein, anti-cystatin C, and anti-β-hemoglobin and rabbit polyclonal anti-prostaglandin D2 synthase were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). In addition, H-ferritin was quantified using immunoblot analyses. One μg of total protein was used for the analysis and a custom rabbit polyclonal H-ferritin antibody from Covance Biotechnology (Denver, PA, USA) was used as the primary antibody. All immunoblot analyses were run in triplicate.
The immunoblots were quantified using densitometric analysis. Chemiluminescent detection was performed using either anti-mouse or anti-rabbit IgG ECL HRP-conjugated secondary antibodies (GE Healthcare). FujiFilm imaging system was used for detection, and densitometric analysis was performed with Multiguage software version 3.0.
Statistical analyses
The Extended Data Analysis tool (EDA) in the DeCyder 2D software package was used for the statistical analysis of the 2D-DIGE experiments. Proteins that were significantly altered were identified by Student's t-test. Protein spots that were differentially expressed (p ≤ 0.05, Av. Ratio ≥ 2.0) between RLS/WED and control CSF samples were identified using MALDI-TOF-MS.
Statistical analyses of the validation immunoblot assays were performed using Student's t-test.
CSF protein profile
CSF proteins showing at least a two-fold difference in expression between RLS/WED and control subjects
Identified protein
Gi access no.
Gene name
Fold change
Proteins that are down-regulated-regulated in RLS/WED CSF
Apolipo protein A-I (precursor)
Major protein component of high density lipoprotein (HDL) in plasma. Promotes cholesterol efflux from tissues to the liver for excretion.
Alpha-1-acid glycoprotein 1
Reduces neutrophil phagocytosis of bacteria, inhibits neutrophil migration and superoxide production, reduces activated platelet aggregation, and inhibits activated lymphocytes proliferation.
Proteins that are up-regulated in RLS/ WED CSF
vitamin D-binding protein (precursor)
Binds vitamin D and its metabolites and transports them to target tissues
Prostaglandin D2 synthase 21kDa (brain)
Neuromodulator and trophic factor in the CNS. Involved in smooth muscle contraction and relaxation; and inhibits platelet aggregation.
cystatin C
Inhibitor of lysosomal proteinases and an extracellular inhibitor of cysteine proteases
most common form of hemoglobin in adult humans
Immunoblot analyses
Prior to this study, only specific iron-related proteins and dopaminergic metabolites have been examined in the CSF of RLS/WED patients [15, 16]. Although the importance of iron in the etiology of early-onset RLS/WED has been established, our proteomic approach in this study permitted identification of proteins that could prove valuable in understanding pathways affected in RLS/WED and identifying targets for treatment strategies.
In this discovery study, we have utilized a 2D-DIGE coupled with tandem MS to identify 6 candidate CSF protein markers for early-onset Restless Legs Syndrome (RLS/WED); four proteins (Cystatin C, Lipocalin-type Prostaglandin D2 Synthase, Vitamin D binding Protein, and β-Hemoglobin) were increased and 2 proteins (Apolipoprotein A1 and α-1-acid Glycoprotein) were decreased in RLS/WED patients compared to controls.
Cystatin C levels were increased in the CSF of early-onset RLS/WED patients. Cystatin C is a cysteine proteinase inhibitor that has multiple functions in vivo. It is known to inhibit both endogenous and exogenous cysteine peptidases, regulate the immune system, have antibacterial and/or antiviral effects; and neuroprotective effects during brain injury. Cystatin C has been described previously as a biomarker of pain in lumbar disk herniation, sciatica, and labor [17, 18]. The level of cystatin C observed in the CSF of patients with these conditions was found to be dependent on the pain's intensity and duration. The increased expression of cystatin C in the CSF from RLS/WED patients is consistent with multiple reports of pain associated with RLS/WED symptoms and increased temporal summation to heat pulses in RLS/WED patients suggesting that RLS/WED is associated with amplified CNS pain processing [19, 20]. Also, RLS/WED has significant comorbitity with a number of pain conditions such as fibromyalgia [21] and headache [22]. The finding of elevated cystatin C in the CSF of RLS/WED subjects may provide an objective measure of the pain associated with RLS/WED in some of the patients. Also cystatin C has been reported to be elevated in response to inflammation; therefore, there is the potential that immune system activation may be occurring in the CNS of patients with RLS/WED. A recent review by Weinstock et al. [23] suggests that some conditions that are associated with RLS/WED have an inflammatory/immune component and that inflammation may be responsible for the CNS iron deficiency.
In this study, we found increased levels of lipocalin-type prostaglandin D2 synthase (L-PGDS) in RLS/WED CSF. L-PGDS is the most abundant protein in the CSF and serves two roles: 1) synthesis of prostaglandin D2 (PGD2) from prostaglandin H2 (PGH2); and 2) binding and transporting small lipophilic molecules. L-PGDS alterations have been reported in a number of sleep disorders including narcolepsy and obstructive sleep apnea with and without daytime sleepiness [24–26] and thus its increase is relevant to sleep disruptions in RLS/WED. We have reported that PGDS is decreased in CSF of monkeys that were iron deficient [13]. Furthermore, the increase in PGDS in RLS/WED CSF is inconsistent with the reports of decreased brain iron status in RLS/WED [14]. We suggest that the increase in PGDS that was observed in RLS/WED patients could be part of a compensatory mechanism. It has been reported that L-PGDS is upregulated in the brains of subjects with hypoxic-ischemic encephalopathy (HIE) [25]. Also, it has been demonstrated in a mouse model of HIE that L-PGDS and HIF-1α expression are co-localized and it was implied that L-PGDS may protect neurons from hypoxic insult by local secretion of PGD2. We could hypothesize, therefore, that the increased L-PGDS in RLS/WED may be a result of hypoxia pathway activation [25]. This hypothesis is further supported by our recent finding of HIF pathway activation in the brains and microvasculature of RLS/WED subjects [27].
In this study, we report elevated vitamin D binding protein in the CSF of RLS/WED subjects. Vitamin D binding protein (VDP) is noted for specifically binding and transporting a number of sterols including vitamin D metabolites to target cells, however, it has been also been shown to be a scavenger of toxins that are released due to cellular injury or death [28] and a modulator of the immune response [29]. Alterations in cerebrospinal fluid levels of VDP have been linked primarily to neurodegenerative diseases, such as multiple sclerosis, Parkinson's disease, and trauma [17, 30, 31]. Vitamin D also has important ties to the pathways that have been identified in the etiology of RLS/WED. Vitamin D deficiency has been linked to altered dopamine concentrations in the cortex [32], and blood and tissue iron content has been reported to increase following vitamin D supplementation [33]. Balaban et al. have reported decreased serum 25-hydroxyvitamin D levels in female RLS/WED subject and have postulated that the CNS iron deficiency that occurs in RLS/WED may induce alterations in the dopaminergic pathway via a vitamin D-mediated pathway [34]. Because of the tie between iron, dopamine and vitamin D, it is tempting to propose that elevated VDP that we observed in this study could be reflective of the alterations in the iron/dopamine pathway. One could also hypothesize that the elevation of VDP could also be reflective of activation of inflammatory/immune cascades in the CNS of RLS/WED subjects as further supported by the alterations of cystatin C and alpha-1 acid glycoprotein levels in RLS/WED CSF.
Beta-Hemoglobin was found to be increased in RLS/WED CSF. β-Hemoglobin is a major component of adult hemoglobin and normally is not present in the CSF. As such, the presence of β-Hemoglobin in the CSF is often considered to be indicative of blood contamination. While, obtaining a CSF tap with no blood contamination is extremely difficult, we have examined the CSF for frank signs of blood to rule out contamination. β-Hemoglobin is known to transport oxygen in the blood, however, it has also been reported to be important in dopamine regulation in the brain [35]. Biagioli and colleagues have reported that overexpression of Hbb-b1 and Hba-a1 (the genes encoding Hemoglobin β and Hemoglobin α) in dopaminergic cell lines alters transcription of genes related to iron metabolism, oxygen homeostasis, oxidative phosphorylation, and nitric oxide synthesis [35]. Because evidence exists for the disruption of both the dopaminergic and oxygen homeostatic systems in RLS [27, 36–38], it is tempting to suggest the finding of increased β-hemoglobin in the RLS/WED CSF may reflect a more chronic condition in RLS/WED and perhaps may point to a mechanistic explanation for the altered iron, dopamine and oxygen protein profiles that are observed in RLS/WED.
Apolipoprotein A1 was found to be decreased in the CSF of patients with RLS/WED. Apolipoprotein A1 is the primary protein constituent of high density lipoproteins (HDL). Although the role of Apolipoprotein A1 in the CNS is not completely understood, it is thought to be important for transport of lipids into the brain [39]. To date, no association has been reported between Apo-A1 and RLS/WED, however, recent studies [40, 41] have described a higher prevalence of hypercholesterolemia in RLS/WED subjects. Decreased serum Apo-A1 levels have been linked to increased incidence of cardiovascular disease [42] which, if the decrease in Apo-A1 that was observed in RLS/WED CSF was reflective of a global Apo-A1 level, this could be related to the increased risk for cardiovascular disease in RLS/WED [4–7].
Alpha-1-acid glycoprotein levels were decreased in the CSF from RLS/WED patients. Alpha-1-acid glycoprotein (AGP) is synthesized primarily in the liver, but also is reportedly synthesized in the endothelial cells of the blood–brain-barrier. Alpha-1-acid glycoprotein (AGP) is an acute phase reactant that is involved in the inflammation cascade and is thought to provide immediate and effective response to inflammatory damage prior to successive protective mechanisms [43]. Their protective functions seem to result mainly from iron sequestration [44, 45]. Our finding of decreased AGP in the CSF of RLS/WED subjects is reflective of decreased iron content in the CNS and provides further support for altered iron homeostasis in the brains of RLS/WED patients [14, 46, 47].
Our results reveal a protein profile in the RLS/WED CSF that is consistent with iron deficiency, dopamine dysregulation and inflammation. The APO-A1 finding may be of relevance outside of CNS given the recently reported increased risk of cardiovascular disease in patients with RLS and cardiovascular dysfunction and reports of painful symptoms. The profiles in the CSF are also consistent with neuropathological findings of activation of HIF pathways and alterations in dopaminergic systems. The data indicate that the CSF protein profile, if confirmed in larger sample sizes, may provide support for existing hypotheses about a biological basis for RLS/WED which could prove clinically meaningful in evaluating therapeutic strategies and identifying new targets.
This work was supported by grants from GlaxoSmithKline (#109851) (JRC).
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- Mizuno S, Mihara T, Miyaoka T, Inagaki T, Horiguchi J: CSF iron, ferritin and transferrin levels in restless legs syndrome. J Sleep Res. 2005, 14: 43-47. 10.1111/j.1365-2869.2004.00403.x.PubMedView ArticleGoogle Scholar
- Connor JR, Boyer PJ, Menzies SL, Dellinger B, Allen RP, Ondo WG, Earley CJ: Neuropathological examination suggests impaired brain iron acquisition in restless legs syndrome. Neurology. 2003, 61: 304-309. 10.1212/01.WNL.0000078887.16593.12.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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The Dickinson, by Stacy Adams Shoes is a cap toe oxford featuring antiqued leather and brogue detail. This classic men's dress shoe represents over 100 years of Stacy Adams craftsmanship and style!!
- Cap Toe Oxford
- Antiqued Leather Upper
- Leather linnings
- Fully Cushioned Insole
- Memory Foam For Added Comfort
- Non-Leather Sole
- 25066-001
Stacy Adams and men who know style have been a perfect fit for over 100 years. Founded in 1875 in Brockton, Massachusetts by William H. Stacy and Henry L. Adams, the Stacy Adams Shoe Company has long been a part of American culture. From the roaring '20s and the post-war boom, to Prohibition and the jazz era, Stacy Adams has been there.
- Item #: 10188

subset: commoncrawl


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Labour decided it should be a women-only candidate list following current MP and transport minister David Jamieson's decision to stand down.
Alison Seabeck was picked by party members on Wednesday night.
Ms Seabeck lives in London and works for Local Government Minister Nick Raynsford. Mr Jamieson won the seat with a 13,000-strong majority in 2001.
The shortlist also included city councillor Sue Dann, lawyer Anne Moore-Williams, party activist Margaret Mythen, and Madlin Sadler, who works for motor company Ford.
Labour said it decided on an all-women shortlist as part of its "long-standing commitment" to redress the balance between the sexes.
Mr Jamieson, 57, has been an MP in Plymouth for the past 13 years. He said he felt he has achieved all he set out to and that it was time to move on.


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Rolling Hills Holidays
UK England West Midlands Shropshire
Wooden Roundhouse
Set on a farm in the Shropshire Hills Area of Outstanding Natural Beauty, this brand new pair of glamping roundhouses was originally created as a handy stop-off point for walkers on the Offa's Dyke path. The national trail passes a mere 300 metres away and the wooden, yurt-like cabins, set on a hillside with sheep-dotted views down the valley, mean you never need to leave the vistas of the trail behind. It hasn't taken long, however, for Rolling Hills to reach a far wider audience. From families wanting a space where kids can run riot outdoors, to couples hoping for a quiet rural retreat, this modern hideaway in the hills has an instant appeal.
Fronted by a gravel terrace, where guests can sit out and enjoy the view, the two roundhouses each sleep up to four people, with a large king-sized bed and a pair of futons that can be converted into single beds. Above, a circular window allows you to peep out at the night sky from the comfort of your abode, while a wood-burner keeps things snug. A wooden-clad communal building, meanwhile, separates the two structures, housing inside a modern kitchen that all guests can use. It also has an excellent new shower room to accompany the roundhouses's individual, en-suite loos.
While each roundhouse has a flat-screen television, the real entertainment at this site comes from its more natural assets. Within a 50-acre field, with a small stream running along one side, the area offers ample exploring opportunity for children, while, for any glamper that brings along an OS map, a world of adventure awaits – linking up with the Offa's Dyke trail or joining one of the local footpaths for a circular route is always a good start. By car, the sleepy little town of Clun is an enjoyable place for a pint or picnic, with its 15th-century stone bridge and ruined castle creating a picturesque framework for the rest of the town, while nearby Bishops Castle has a clutch of museums and a market in the town hall most Saturdays.
The Owner SaysRolling hills holidays is a small glamping destination tucked away in South Shropshire next to the Welsh border in an unspoiled valley surrounded by hills. A local family wanted to create a unique glamping site with roundhouses to share their love of the location with others. Rolling hills holidays is where adults can escape and find peace and children can get back to nature. The site is within a 50 acre field with a small stream running through it completely surrounded by farm animals, wild birds, rabbits, badgers and even deer at times, who go about their daily business. We wish to preserve the peaceful nature of the location so we all can continue to make fond memories just like we do with our family.
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Rock Notes
Madison Tierney's a writer, whose husband, Thomas suddenly left her after 10 years; she is destroyed and now recently alone and divorced. She picks herself up slowly trying to restore her lost confidence and ability to love again. She follows her writing passion and ventures to follow a local band - Rolling Isaac's and write about them, raw and real and how they follow their dreams. Her book is titled ROCK NOTES.
Her ex-husband wants to come back, and she is torn with what she had for ten years with him which was safe, comforting and a committed relationship. And now she is falling hard for the band singer - Max Rand (Madison calls him Rand), who is several years younger than her and every moment with him is intense and unpredictable.
Rand has tragically lost love in his life. He has no love left to give. He fills his time with many groupies and late nights. The story travels you to various concert venues, from a sexy piercing party to a New Years rocking eve. There are many interruptions and assumptions for this couple along their journey.
While Madison is writing ROCK NOTES, somewhere along the way she begins to write LOVE NOTES, which are personal secret love letters for Rand. Also Rand is always writing music with the hope of creating his first solo album. Both Madison and Rand appear to be able to write their feelings on paper effortlessly but have a hard time conveying them to one another. This story is about being so broken inside from having your heart ripped apart, to attempting to rebuild trust, follow your dreams and hopefully find love.
Will she return to the warm awaiting arms of her ex-husband who still is in love with her? Or will there be a new beginning for Madison and Rand? Can these two empty souls feel love again? Or will what ignites between them simply fade away after the concert lights dim?
Purchase Rock Notes from:
Love Notes
As our bodies joined as one, I felt more in that one moment than I have ever felt with anyone. I never wanted to leave your arms, your body, and our connection. I believe that you complete me, you were made for me. I struggled to keep my feelings at bay and not let you know but I am too emotionally involved with you and to know now that you are with me is beyond belief. Where we head from here together has unlimited paths. I know I will always have this need now to have you fulfill me as I know you can. I see you and just a glance across the room to you makes me want to have us running to the bedroom. Silently I ponder how to relay to you just how much Love I contain for you. Some days I feel like my heart is about to burst. Maddy xo
Madison and Rand's love story continues as they move their relationship to a more passionate, intimate level…but again they are thrown some challenges. Will Madison and Rand be together and move forward or…backward? Read what comes their way to cause doubt in their minds. Just when everything is going fine, sometimes accidents occur.
Oh, God, something horrible has happened. I only remember parts of it and they seem like snapshots that were taken so quickly…I need to feel you near me. I need to reach out and touch you and let you know I love you. Rand, I never felt so in love as I do when I am with you. Maddy xo
"What the hell are you talking about Madison? You have me at a complete loss here?" Rand
Madison must fully trust Rand to commit herself to him forever in marriage. Rand must make her see and feel what she truly means to him. This story will pull at your heart and make you tear as you turn the pages that may finally reveal Madison's handwritten LOVE NOTES.
Will they get their Happily Ever After?
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Music Notes
How will Madison and Rand keep their love and music playing?
Music Notes (Book Three of the Heartbeat Series)
Just when life seems to be going smooth, Madison receives news that leaves her speechless. She's a writer that for the moment cannot form her words. Rand now is the one to continue their story…his story.
Music Notes will take you on the tour with Max Rand as his first Solo Album – Simply Mad is launched. Will his travel for several months put distance in their relationship? Can their love survive?
Rand will travel through his own journey of self discovery along the way. Just when Rand is at a high point in his life, a collapse . . . will bring one of them down...Rand? Madison?
One person will offer the ultimate sacrifice. See the songs that Rand has written, and where his inspiration comes from.
Follow this novel and see how it plays out.
Purchase Music Notes from:
Check out the Music Notes Trailer :
Author that has the passion for putting her pen and ideas to paper. A pure romance junkie and she loves to tell stories. She was an English Major of LaSalle University and always wanted to be a writer. In the past years her books titled - From the Vine (a collection of writings/poems) and Cody and the Pumpkin Truck (a Childrens' Book) were published. She has also written many other children's stories, lyrics, articles and wedding ceremonies. Currently she is deep into her Heartbeat Series of Contemporary Romance Novels which are titled - ROCK NOTES, LOVE NOTES, MUSIC NOTES, FIRST BEAT, FIRST BASS and FIRST TASTE.
Also coming soon The Crossing Series I, II and III of The Knot Hole, The Passage and The Muse.
Renee resides in Eagleville, PA. with her husband Michael, of many years and her two cats - Nyah and Cody. She has a great support system of Love from her family and friends.
Renee BELIEVES you should follow your DREAMS and that -
The HAPPIEST of people don't have the best of everything,
they simply make the best of everything.
Renee loves to travel, especially to St. Martin - Netherland Antilles. She enjoys meeting new people to inspire her and she will always write down a person's name that is unique to use as a potential character in her future writings.
Places to find Renee Lee Fisher:


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Can thinking about smartphones keep you awake at night?
We've all been kept awake at night thinking of the days activities or things we are looking forward to in the future.
A new study has shown that smartphones may be another factor to think about when it comes to factors that can disrupt sleep patterns – and this is particularly the case when it comes to children.
The study, which was led by researchers from King's College London, revealed that children have a higher risk of a disrupted night's sleep if they use electronic devices such as smartphones or media tablets before bedtime compared to those who refrain from use in the late hours of the evening.
According to previous research, 72 per cent of children and 89 per cent of adolescents keep at least one device in their bedrooms – and most of these are used near bedtime. However, the speed at which the uptake of these devices has increased and the technological development of these products outpaces the quantity of research produced in the area, meaning that the impact on sleep is not very well understood.
The new data takes the form of a review of 20 existing studies from four continents. It has been published in the scientific journal JAMA Pediatrics and analysed data captured from 125,000 children aged 6-19, with an average age of 15.
It was established by the researchers that bedtime use of media devices was associated with an increased likelihood of both a lower amount of sleep and poorer quality of sleep, in addition to excessive daytime sleepiness. For the purposes of the data, bedtime use was classified as engagement with a device within 90 minutes of going to sleep.
A media device being present in the bedroom – even without use – was also associated with a higher chance of poor sleep. Rather than this being the result of any direct effect, it was suggested that one potential reason for this trend was that the 'always on' nature of social media and instant messaging means young people are always engaged with these devices in their environment, even when they are not in active use.
King's College London's Dr Ben Carter said: "Our study provides further proof of the detrimental effect of media devices on both sleep duration and quality.
"Sleep is an often undervalued but important part of children's development, with a regular lack of sleep causing a variety of health problems. With the ever growing popularity of portable media devices and their use in schools as a replacement for textbooks, the problem of poor sleep amongst children is likely to get worse.
"Our findings suggest that an integrated approach involving parents, teachers, and healthcare professionals is necessary to reduce access to these devices and encourage good sleeping habits near bedtime."


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Dr. Maria Cristeta N. Cuaresma, Program Head for Graduate Education and Institutional Development (GEIDD), and Dr. Maria Monina Cecilia A. Villena, Special Projects Coordinator and Network Administrator of SEARCA's Biotechnology Information Center (BIC) under the Knowledge Management Department, introduced top officials of private HEIs in the Philippines to the agricultural biotechnology landscape and its latest course offerings in the country and Southeast Asia during the Top Management Program for Private Higher Education Institution (HEI) Presidents and Chancellors: Crafting New Strategies for the K-to-12 Transition Years (2016-2021) organized by the Asian Institute of Management through the Stephen Zuellig Graduate School of Development Management (AIM-ZSDM) on 12 January 2016 at AIM in Makati City.
Dr. Cuaresma introduced the mandate and activities of SEARCA and the SEAsian University Consortium for Graduate Education in Agriculture and Natural Resources, including the program offerings of the Consortium in biotechnology. Dr. Villena then presented the global and local status of biotech crop adoption, as well as its potential and documented benefits, and introduced the BS Agricultural Biotechnology Program in the University of the Philippines Los Baños (UPLB). She also briefed the participants about the activities of BIC, which aims to address the needs of the region for a highly credible, sound, and factual source of biotechnology information accessible to various stakeholders.
The training program sought to develop the participants as transformational leaders in three areas: Institution Building, Academic Management, and Resource Mobilization. It was funded by the Philippine Commission on Higher Education (CHED). (Sophia M. Mercado)
Southeast Asian Regional Center for Graduate Study and Research in Agriculture
College, Los Baños, Laguna 4031 Philippines


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yoni206 06 Jun 2010 05:54
In a previous post, it was said that evey graph has a clique of size one.
How come? as I understand it, for a graph to have a 1-clique it must has a loop. Am I right?
ant about k=0, every graph has a 0-clique right?
And another thing about Q6:
May we assume the graphs are simple? or maybe even that k>1?


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We are in the middle of a quixotic quest to build a profitable strategy based on skew (parts 1 & 2).
Today, I added a horizon feature to the code, so now we can trade monthly; which matches the 30 day forecast the skew index gives us.
Will we finally strike gold?
The monthly Sharpe ratio has now nudged up a little to 0.28; putting it another way, our strategy has a lower (but not very low) 8% chance of being jibberish.
I need to take a cold hard look at the relationship between the S&P 500 and the implied skew index.
Also, our strategy shorts the S&P 500 at the smallest sign of trouble; when most of the time it would be better to hunker down and increase our cash holding.


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Division of Federal Employees' Compensation (DFEC)
Enhancements to OWCP's Medical Authorization and Billing System
We have recently enhanced our computer system to ensure that the care the injured worker is receiving is relevant to the condition(s) for which the case was accepted. These enhancements were made in accordance with the Federal Employees' Compensation Act which mandates OWCP to furnish an injured worker with services, appliances, and supplies prescribed by a qualified physician which OWCP deems likely "to cure, give relief, reduce the degree or the period of disability, or aid in lessening the amount of monthly compensation." While we have always had processes in place to help assure that OWCP authorizes and pays only for services, treatments, medications, and durable medical equipment related to the accepted conditions on a claim, our recent computer enhancements provide more rigor to these processes.
As a result of this, some injured workers may find that treatment or medications previously allowed are no longer available to them. If an injured worker's physician believes a treatment, procedure, or medication is necessary for the treatment of the injured worker's accepted conditions, the provider needs to submit medical documentation for review by the claims examiner. As is the case with anything sent to OWCP, please be sure to include the injured worker's claim/case number on every page. Please mail all documentation to U.S. Department of Labor, DFEC Central Mailroom, P.O. Box 8300, London, KY 40742-8300.


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Skip To Navigation
Skip To Content
Alberta.ca > Agriculture and Forestry
The Rural Development Division, Alberta Agriculture and Rural Development, set out to answer these and other questions when they contracted The Conference Board of Canada to determine the economic contribution of rural Alberta to the rest of Alberta and Canada.
Results include rural GDP, employment by place of work, and "economic footprint" comparing 2001 and 2006 Census data. The results are broken down comparing urban and rural (using the "Rural and Small Town" definition), Economic Region, Census Division, Metropolitan Influence Zone (MIZ), and by 18 industry sectors. Estimates of community level GDP and industrial structure were also calculated for 317 Census Subdivisions (municipalities), a first for Alberta.
Read the full report.
Return to Rural Development homepage.
Phone the Ag-Info Centre, toll-free in Alberta at 310-FARM (3276), for agricultural and forestry information.
© 2012 - 2019 Government of Alberta Copyright and Disclaimer


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Here is the latest (that I know of), taken directly from notes of the Tejas Miata Club May meeting of 5/15/2017:
● The Texas Roundup being hosted by Tejas Miata Club will be held Nov. 3rd - 5th.
● Planned scheduled will look something like this:
○ Thursday - early registration / check-in / self-guided tours / runs
○ Friday - Registration / Informal Runs / Movie at Blue Light Drive Inn
○ Saturday - SASCA AutoX / ½ day runs / Dance Hall evening meal, dance,
○ Sunday - Thank you and departing events (funtana event?)
● Volunteers needed. This is our event!
And more from that meeting:
The Holiday Inn Express is Buda is the official
Roundup hotel. There is
a block of rooms reserved. We will have AutoX event as part of the Roundup
hosted by SASCA. The Saturday night venue will be - Pinballz Kingdom - which
can accommodate lots of people and will host a dinner/party Saturday night.
Finally, we are planning numerous runs to give all attending members an
opportunity to go drive. We need ideas and volunteers. As the hosting club,
please help with the planning. Cost is currently working out to $70 - $75 a person
but still needs to be finalized before we open up the Roundup site for registration.


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I just want to focus on one point that was deeply imbedded in the talk. It is a idea that is profound and central to the physics of quantum electronic circuits. The idea is so old now its profoundness and brilliance may be lost on a new generation.
The idea and result is easiest for me to explain in terms of the figure below which describes a superconducting (Josephson junction) qubit connected to an electrical circuit. It is taken from this review.
One can quantise the electromagnetic field and consider a spin-boson model to describe decoherence and dissipation of the qubit. This is associated with a spectral density that is proportional to frequency with a dimensionless pre factor alpha, which for this circuit is given by
Similar physics is at play in normal tunnel junctions (see for example this important paper, highlighted by Matthew in his talk).
Why do I find this profound?
First, this is a very simple formula that depends only on macroscopic parameters of the electrical circuit. One does not have to know anything about the microscopic details of all the different electronic degrees of freedom in the circuit or how they individually couple to the qubit. I find this surprising.
Second, the underlying physics is the fluctuation-dissipation theorem. The quantum noise in the electronic circuit is related to fluctuations in the current. By Kubo and the fluctuation-dissipation relation tell us the fluctuations in the current are essentially the conductivity [the inverse of the resistivity].
Who was the first to have this insight and calculate this?
I feel it was Caldeira and Leggett, but I can't find the actual equation with the circuit resistance in their 1983 paper.
Or did someone else do this earlier?
Because of the above, whenever the spectral density depends linearly on the frequency, Leggett (and now everyone) calls it ohmic dissipation.
I first learnt this through the thesis work of my student Joel Gilmore, and described in this review. There we considered a more chemical problem, two excited electronic states of a molecule that are in a polar dielectric solvent. The coupling to the environment is completely specified in terms of the frequency dependent dielectric constant of the solvent (and some geometric factors).
Update: Caldeira answers the question in a comment below.


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Transient Ischemic Attack and Stroke
Accurate diagnosis, appropriate investigation, reliable risk stratification, and urgent treatment are essential after TIA and minor stroke and can reduce the risk of stroke recurrence by up to 80%. This book reviews recent developments in each of these areas. To reflect the fact that TIAs and minor strokes are simply one end of the clinical spectrum of cerebrovascular disease, the book also covers the management of major stroke, and long-term outcomes - such as cognitive impairment - which can complicate all types of cerebrovascular events. The book is therefore a comprehensive primer in stroke medicine. Written by a leading team of researchers in the field, this book will be of interest to neurologists, stroke physicians, and all others with an interest in stroke.
Weiterlesen weniger lesen


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Jingling Geordie AT 2014 – George Heriot's School CCF
Twenty six members of George Heriot's School CCF spent six days of their October break embarking on Adventure Training in the Lake District. For many it was their first experience of adventure sports outside and every member of the party was looking forward to the challenges which lay ahead. The expedition had three main objectives; teamwork and team bonding; to learn new skills and to become enthused by the outdoor world – whilst, of course, having fun!
Travel to the Lake District was without incident and it was not long before the cadets were settled in their new home for the week. Based in Patterdale, at the Langton Adventure Centre, the group was well situated to make good use of the surrounding opportunities. The staff and training providers were extremely thorough in their preparation of each day's activity; ensuring safety and progression were always paramount.
Each day, three separate groups set off to undertake different activities. These included canyoning; climbing; scrambling; abseiling and via ferrata and offered the chance to learn new skills and reinforce previous learning. Many of the cadets had cited a fear of heights in a pre-expedition questionnaire and this was successfully addressed by many during the trip.
Canyoning proved to be a very popular experience for many. Although most were very cold by the end (it was October!), everyone had a fantastic day out descending the ghylls and GHS CCF would recommend anyone to give this particular activity a go.
Via ferrata was also a very popular event – although much shorter than the routes that are found in Europe, this experience was widely championed and many have come back to Edinburgh with the plan of convincing parents to take them to the Dolomites on family holidays!
During the evenings the cadets were not left to rest on their laurels and were involved in group activities; problem solving; team games and a night walk. The expedition came to a crescendo on the last day of training when the group visited the very atmospheric Cathedral Quarry, where they were challenged by the 120ft abseil into the quarry and extraction through the old mines.
The CO and expedition leader were very pleased to have met all the aims of the expedition and felt that they had exceeded their expectations and achieved a great deal of citizenship training, as well as compounding military training.
It is now the Training Officer's intention to encourage cadets to book onto Adventure Training courses with Cadet Centre for Adventurous Training (CCAT).
Ulysses Trust must be thanked for their generous support and financial backing, without such the cadets would not have been able to have such a tremendous package and fulfilling week.
Mention must also go to the external instructors, led by the very accommodating Alyn Griffiths of High Points and supported by the excellent Ben Bryant.
Captain M Hawkins
Training Officer
George Heriot's School CCF


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The management report is rascal's key top-line summary report for retailers.
It gives simple, accurate and up-to-date visibility of stores' and suppliers' performance, across key metrics, during the delivery, receiving, returning and crediting processes.
This allows customers to easily measure performance, identify areas requiring focus and facilitates control of the News and Magazine category.
The report is split into detailed tabs which focus on the category sub-sets; returns and deliveries, separated out by newspapers and magazines.
The non-range report is rascal's primary tool for highlighting range and supply problems across a retailer's estate.
It gives simple, easy to understand visibility, broken down and ranked by supplier, publisher, title and store performance. The report highlights the scale of titles delivered into stores which were not part of the range as well as those retained incorrectly and not returned.
This allows the retailer to challenge both the supplier and in-store processes and improves control of what goes in and out of stores.
Where appropriate the report is split into detailed tabs which focus on different retailer store formats (main estate, convenience, petrol filling stations). The report includes weekly analysis trending on the key metrics.
Poor process compliance when booking in and returning stock is the key contributor to category shrink. This simple and easy to use report gives management visibility of stores' performance and areas to focus on which will improve the overall control of the category.
The report can be configured according to customer requirements but typically breaks stores down into clusters such as by regional or territory managers.
The Daily Store Compliance tab shows a breakdown of store scanning performance as well as whether they meet timeline targets for uploading newspaper or magazine delivery shortages which are time sensitive. Any claims for shortages raised outside of these timelines are unlikely to be credited.
The Weekly Store Compliance tab shows process activity across the week and using Red Amber Green colour coding highlights if stores have met pre-defined company KPI's for performance. It also shows rolling month to date average compliance and trending.
rascal's comprehensive Business Objects reporting tool provides the ability to easily produce simple customised reports giving visibility on any given key metric area.
These reports can be built from scratch and to customer requirements.
The process of creating the reports is as simple as taking the key metric variables, dragging them into a table and using filters and calculations to give the desired visibility and results.
Our team of account managers are comprehensively trained in all the skills required to produce ad-hoc reports as well as understanding the complexities of the data relationships.


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Millington School Threat Leads To 17 Guns Seized and Two Students Arrested [Video]
The Millington School district shut down early for the year because of numerous violent threats made, and now authorities are finding out that some of the threats were very credible.
17 guns total have been seized and two Millington freshman are in juvenile custody, with one of them facing terrorism charges. ABC12 is reporting that the guns were seized from the 15 year old who is facing the terrorism charges.
Also charged in this investigation is a 16 year old. He faces an assault with a dangerous weapon charge. The 16-year-old's stepfather tells us the weapon was a razor from a broken pencil sharpener. According to police, one of the 16-year-old's victims is the 15-year-old, who police say made the threat of terrorism against other students and the high school.
This is as scary as it gets as far as I'm concerned. The school and police are lucky to have found out about the possible threat before we had to deal with another tragedy close to home.


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Want to collaborate?
We seek visionary funding partners, inspired thinkers, institutional and community allies, researchers, storytellers – whomever feels called to be part of this potent, joyful collection of loving, curious people serving unmet needs wherever they are found. We seek to grow new, fruitful alliances, lift up the best of who we are, liberate hidden common-wealth in people and communities, and nourish all we hold in common. Be in touch! Reach us at email@example.com.


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It began a long time ago when I lived on a farm and the farm cat was killed, leaving her kittens orphaned. Small as hamsters, I begged the farmer to let me have them, begged him not to drown them in a bucket. Over sleepless nights, with the help of a good vet, I managed to get one through the dangerous early stages and raised the biggest white cat in the world. And in doing so I fell in love with all of catkind.
So, here are the cats that I have so far measured out the days of my life with. Comfrey, big, bold and white, then Arthur and Martha. Martha was a gentle soul, Arthur a great walker. Bird came to live with us one day having watched and waited, looking for a good place to live. From the first day he and Bella loved each other very much. Then Kiffer who walked with his head on one side, possibly the strangest creature I have ever met. Then Pixie and Maurice. All these are gone now, and at the moment only Elmo and Max live here. Max is in many of my books and has lived with us for 13 years. Elmo is just a bit naughty.
And today Elmo decided it was time I got on with making a page for the book and that he should help.
Most books don't have a clear beginning. This one grew out of watching the cats sleeping and wondering about the dreams of animals and wanting to do a book with lots of big cats. The words came together over a few walks with the cats. They prowled through notebooks and then I began to doodle in my sketchbook. With books like this I try to use as few words as possible. The words are a catalyst to stories and dreaming.
Trying to work out how to make the book work was a little difficult. There are far more species of cat in the world than there are in the book. Some were obvious contenders. The Scottish Wildcat is so little known in Britain and there are so few left. There had to be cheetahs, and tiger and lion. At first there was a child at the end of the book, cuddling a cat, but I thought it would make it more inclusive somehow if there was just a cat, so the child reading the book could have this cat as their own. ( When I was a child I was hungry for a cat creature and not allowed to have one. So, I roamed in my imagination with Bagheera, with Carbonnel, and Gobolino, and the cat that walked by itself).
From the sketchbook I moved to a full sized dummy book. This is the way I usually work when making a picture book, drawing it all out so that I can see how it works as the pages turn. ( I always do dummies in black and white, though other illustrators I know make the most glorious dummy books in full colour. Just beautiful. And I have dummy books for Song of the Golden Hare, I am Cat, The Snow Leopard, Mariana and more. Hoping to send a few to Seven Stories for their archive soon) Sometimes the pictures stay the same from dummy to finished piece. Other times they change.
( Secret- I was watching A Knight's Tale with Heath Ledger whilst doodling the lion spread. The sketch for the lion looks really rather too much like him. Funny how what we see influences the way we draw!)
Originally the book was called I am a Cat but in conversation with my friend Eva John she suggested that the title would be much stronger if I dropped the 'a'. I have learnt a lot from Eva over the years, and this was a great suggestion. Amazing how much stronger a title can be with the removal of just one letter.
Once the dummy book was completed and I had talked through the book with my editors all that remained was to get on with working on the finished pieces and try to paint a striking and arresting cover that would fit in with other books in what is becoming a sort of series. I prefer the French edition with the close up of the cat face. It is also 25% larger than the UK and US version.
The Korean edition is so different.
The endpapers show a small cat walking in the moonlight. I have been lucky with cats. They have always come for walks with me, for miles on the Pembrokeshire coast path. Years ago I began a blog with the cats. They looked so beautiful in the wilds and the different seasons of Pembrokeshire. Pixie in particular would walk for miles with me, and now Elmo likes to come to the high rock above home where I go to write. The cat blog still lives, in cyberspace. Sadly Maurice and Pixie are gone. And now Elmo has a facebook page for I am Cat. All the images for the book can be seen at The House of Golden Dreams.
Below are some of the many hundreds of images from Wethreecats.
So, it was while out walking that I am Cat was born, but also it was watching cats sleep.
Watching cats sleep, hearing their dreams, and seeing them curled, ammonite tight, in curious places.
I am Cat by Jackie Morris is published by Janetta Otter-Barry books, Frances Lincoln in the UK and USA isbn 978-1-84780-135-7
It is dedicated to Marilyn Brocklehurst and her cats at the Norfolk Children's Book Centre, and also to all of the cats who have graced me with their company and all the cats yet to come in to my life.
Here is a link to a soundfile of my talk at Hay Festival all about I am Cat and other books, and writing and painting and cats.
Review for I am Cat from IBBY Notes: by Shirley Hobson
I Am Cat
Jackie Morris, London: Frances Lincoln Children's Books, hb. 978 1 8478 0135 7, £11.99, 2012, 32pp.
This picture book will appeal to cat lovers of all ages.
It is a beautiful book to hold and look at with its luminous watercolour illustrations packed with hidden images. There is something different to see each time a page is revisited.
The story is simple. A domestic ginger cat is curled up, warm and contented in its bed and is dreaming. We enter the cat's dream and together we go on a magical journey, visiting the various places inhabited by nine wild cats. We are enchanted by what we see and learn through the eyes of the familiar sleeping cat. It is with wonderment that we find out how these other cats live and try to survive in their environments. It is made clear how endangered they have all become, apart from our domestic cat.
Jackie Morris' brilliant illustrations and carefully chosen words alluding to the often mysterious worlds these amazing creatures inhabit are easily understood. The way of life of big cats such as the Siberian Tiger, the Cheetah, the Lynx, the Puma, the Jaguar, the Snow Leopard, the Lion and the very rare Amur Leopard are all in the dreams of our ginger cat. However, it is not only about dreams of 'big cats' in exotic places. More unusually there is a lovely double-page spread where our cat dreams of being a 'secret cat' in the mountains of Scotland. We learn that this cat is 'striped like a tiger, solitary and fierce, ancient, almost a memory'. This cat is also in danger of becoming extinct.
The text is sparse. The author doesn't waste words and includes some quite difficult ones for small children, like ammonite, when describing the way our dreaming cat is curled up. The child will learn new words to memorise for use later on. There is also, after the story, a really good spread showing all the cats with a brief résumé relating to the animals in real life and the dangers they constantly face.
This unique celebration of cats is not only a beautiful work of art for young children to enjoy, it is also extremely well researched and very informative."
Signed copies of this and all of my other books in print are available from Solva Woollen Mill.


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2013 brings two new shows to Windborne Music and the first one is the Music of Whitney Houston. This show becomes our second in what we are calling our Legends Series. The Music of Michael Jackson started us off in 2011 and it was so successful that we had to add one of the world's favorite stars, Whitney Houston.
Music Legend Clive Davis called Whitney Houston "A talent beyond compare."
Whitney had a remarkable singing ability, a powerful voice and unique delivery that touched our hearts and souls. A singer, an actor, producer and model, Whitney had unmistakable talent. A true Diva, the Guinness World Records named Whitney Houston as the most-awarded female act of all-time having sold over 170 million albums, singles and videos worldwide. Her appeal extended to all ages, races, backgrounds and continents.
Broadway sensation Rashidra Scott joins Windborne Music to celebrate this amazing woman and her extraordinary talent with an evening of Whitney's biggest hits… "Saving All My Love," "How Will I Know?," "Where Do Broken Hearts Go?" and of course "I Will Always Love You." The Music Of Whitney Houston: A Celebration.


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With Sixt, luxury car rental in Kuwait is simple and affordable. Our car rental branch in this city is situated in Kuwait airport and here you can rent luxury vehicles from brands such as Audi, BMW, or Mercedes. Sixt luxury cars are perfect for making a big impression at an executive trip or adding elegance to a honeymoon. If you would like your premium vehicle for several weeks, or over a month, calling our Kuwait branch in advance is advisable, as then we can confirm that we have the luxury car you have chosen available for the right amount of time. Sixt also provides cheap deals on insurance, extra drivers, and unlimited mileage.
Sports car rental and other types of luxury car hire ensure that you will make an impression wherever you go. The Kuwait Towers, the Grand Mosque, Liberation Tower, and all of the famous sights of the city are so easy to get to when you have a luxury car at your disposal. Lovers of culture and history should make sure to park up outside the National Museum, whilst travelers with kids should not miss out on the opportunity to take a short drive to visit the city's Zoo. The Science and Natural History Museum is also well worth a visit whilst the Souq al Mubarakeya is a great place to buy some souvenirs.
Kuwait International Airport - Phone # +965-98-921129
Address - Arrival Hall, Basement, 13001 Farwaniyah, Kuwait


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An Anti-Climatic Answer
Go down one more post.
Come on now.
I'm waiting...
This answer isn't half as fun as the ones I got. I thought the middle donut resembled a praying alien.
Do aliens pray? If so, what do they call their god?
Maybe his prayer went like this.
Dear Zanzub,
Please, please let our domination of Earth go well and if it's not too much too ask, I would be thrilled if people tasted like chicken.
And the donut to the top right of the praying alien looked like a sperm cell to me. The one to the top left looked somewhat like a padlock, more so than it showed in the picture.
The fun part was we all saw different things.


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Congratulations to my seller on the sale of his vintage farmhouse out in the Kings Valley/ Wren area of Philomath at 37963 Kings Valley Highway. Wishing him the best in his next adventure! … [Read more...]
SOLD! 37963 Kings Valley Highway, Philomath
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With Liberty and Justice for Not Everybody
So what about those great and stirring words "with liberty and justice for all?" Doesn't that phrase appear in some place that is so important that the words have been permanently etched on the American psyche from childhood? Maybe it's a part of the Flag Salute, aka the Pledge of Allegiance. My mind wavers, because I've had no occasion to stand in a classroom or anywhere else and put my hand to my chest and recite all that, since long before the words "under God" were so infelicitously added (which appears to have happened in the early 1950's. By that time I was in the military, where we did a lot of saluting but hardly any reciting, unless it was the 10 General Orders, and since I also long ago lost my "Airman's Handbook," I no longer can put my hands on what those were either. Isn't it great to have grown so old and casual!)
The Incurious Sanford Jury that delivered that Zimmerman verdict sent the world a more modern message as to whether those words still pertained. "With liberty and justice for all? No, no way! The long-standing view and policy that those words do not apply where nigras are concerned is still in effect."
Once more I am just as glad as I can be that I'm not part of that dominant, Europeno demographic in whose name so many racial injustices have been committed ever since they decided to bring in the first slaves from Africa, to do all that hot, dirty, difficult work clearing fields and tending the cotton and tobacco crops in Virginia, not to mention the myriad other atrocities that were involved in maintaining slavery. And I don't see how anyone in that white superiority demographic with even as little as one drop of decency can sleep easily tonight or any other night, knowing how once again a great injustice has been done by them and in their name, in Sanford, Florida last month, on top of the billions of others that have been committed upon the slaves and their descendants for nearly 400 years.
In the wake of the Sanford verdict, the American rightwingers hoped against hope that, in protest of the verdict, "black" people would react in the style of Pavlov's dogs and repeat what some did in the mid-20th century, especially when Reverend King was shot, and pour out into the streets of all the big cities and start wrecking and burning things. After Sanford all the millions of guns and the thousands of tons of ammunition that the dominants have been buying like crazy could -- this time -- be put to good use. The conservatives looked forward, this time, to the good ol' race riots of yore -- New York 1863, Wilmington N.C. 1898, Tulsa 1919, and many other killing grounds participated in by "the people." The "race riots" of the 1950's and 1960 were not really that at all, but instead could more properly have been called "property riots" and "vandalism and looting expeditions," because in those events ordinary "white" citizens stayed home in their comfortable suburbs, and they let their police do all the shooting and killing. This time the conservatives looked forward, though in vain, to the prospect of ordinary citizens taking part in all the fun once more and causing the gutters in the inner cities to overflow with "black" blood. In conservative eyes, that would've been truly the new "America the Beautiful," and because you would've been talking about the world's only superpower here, no one elsewhere would've had anything to say about it, at least nothing that need be respected.
"[The Negro] had no rights which the white man was bound to respect."
--So said the chief U.S. Supreme Court Chief Justice, Roger Taney, in the 1857 Dred Scott case, midway (so far) through the country's career.
I hope the renewed dread and dismay that I personally feel today can be understood. The overwhelming majority of Americans have never had the experience of being, as I was many years ago, a black youth of 17 not all that different from a Trayvon Martin. And added to that, I had a son who was also at one point a "black" youth of 17, who was also not much different from Trayvon Martin. And it shouldn't be forgotten that the current President of the U.S., B. Obama, said right after the murder that he could've had a son like Trayvon Martin.
Having one's existence valued so cheaply by so much of the rest of the world, even by one's own contemporaries of the same physical description at far too many times, no matter how much that person may try to be benign and law-abiding, is a discomfort that I would never wish on anybody. Still, today we have nearly half the country eager to keep my demographic (chiefly but not always a much younger sector of it) always and forever under the easy-to-use hammer of ever abundant firearms and the often irresistible urge to use them.


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Members of the Year 11 Boys Football Team achieved an early Christmas present by reaching the final of the U16 Suffolk County Cup just prior to going on Christmas holidays.
The Academy side faced a very good Stoke High School in their semi final fixture. Despite not having any ITFC Academy players they are a very strong team, with each of their players being an extremely good footballer which makes Stoke a very difficult team to break down and score against.
In the first ten minutes Stoke started the game much better than our own team with some excellent football, stopping us from playing the great football we have shown for the past three years. After 15 minutes Stoke gained a corner which was cleared by the Felixstowe defense, allowing the Academy side to immediately counter-attack. This move ended with Charlie Hitchen scoring the first goal of the game. This goal gave the whole team much needed confidence after a slow start, and from this point our team were in control of the game.
Jake Crumlish and Charlie Bance, both playing in central midfield roles, took complete control of the game and stopped Stoke's two fantastic midfielders who had taken control from the start. Felixstowe Academy were able scored again before half time after Jack Moss linked up with Sam Ford and scored a fantastic goal after he had beaten two other players.
Jack Moss put in a brilliant performance throughout the entire game; this was his best game for Felixstowe Academy, and won him the Man of the Match award. In the second half the game opened up a little more with both teams creating chances. But the Academy side held their nerve and scored two more, making the score 5-2 to put us through into the Final of the Suffolk County Cup, which we won last year.
Although the Cup Final opponents are still to be confirmed, the whole team have every intention of retaining the trophy this year!
Congratulations to the entire team:
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Hello SOTGC community,
I'm am so excited to be introducing myself to all of you and really honored to be part of this team – providing insights and sharing experiences about living a successful and meaning-filled life! I hope you will find my contributions informative, supportive and fun to read as we go on this journey together. If and when you have comments or questions, please know that I will be ready to engage and happy to chat!
My name is Lizabeth and I am an advocate. I support people with impulse control disorders, which is really to say that, when you are in my company you are safe to discuss the quirks and habits that you use to deal with stress and emotion. SOTGC is excellent about treating each of us with care and dignity – that is one of the reasons why I'm so happy to be asked to 'join the family'. Here specifically, you can share the 'nicks in your armor' and be confident that you will not receive unsolicited advice, get judged or corrected or made to feel shame in any way. If you are in search of information and resources to help curb those behaviors or to adopt new coping skills, I'm happy to help, but if you simply want to say "You bet I'm Superwoman, but what people don't know is that I do XXXXX when I'm stressed….", you won't get an earful of reasons why you need to change what you're doing – not from me. You're already grown up and you know what's best for you so you don't need me to yammer in your virtual ear about "should" or "best for you" – those are YOUR decisions.
What you WILL get is guidance if you want it, information about various stress relieving behaviors that are common to high achieving, type A personalities, insight about some of the reasons why we engage in these behaviors and a lot of great feedback from leaders in the clinical and research arenas of binge eating disorder (BED), Trichotillomania (TTM), body focused repetitive behaviors (BFRB) and compulsive shopping, to name a few.
You will also learn that, as shame invoking and private as some of these behaviors are, they are common. If you participate in any of the above activities, you are not alone. Far from it.
So, like the spa or the salon, the information we share here is about support and empowerment and sisterhood. I will guide as best as I can, but I would also love to know your thoughts, ideas and areas of interest!
Because I'm asking you to trust me and to feel free to share, it's only fair that I start so here is a little bit about me.
I live in DC with my husband and my German Shepherd, Noodle – both of whom have been through the highs and lows of my history with binge eating disorder (BED), drinking, sobriety, and hair pulling. I have many years of experience working in the non-profit sector and I decided a year ago to start my own advocacy and support website called BingeBehavior.com where I gather resources to support people who experience binge eating disorder and various body focused repetitive behaviors. In addition to supporting the behavioral experiences, I provide coordination services for online awareness campaigns such as Weight Stigma Awareness Week (#WSAW) for the Binge Eating Disorder Association and a successful campaign petitioning the White House to consider addressing weight stigma as part of the Let's Move! program. My work in advocacy is strongly associated with ending the stigma that comes along with having disordered behavior so I am active in groups that develop policy to counter it.
So, cheers to starting a new relationship and soon to be a new year! I am looking forward to sharing information, ideas and most of all support with each of you!
Thank you for opening your eyeballs and email to 'meet me' today – Lizabeth


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Step 1Heat the oven to 350 degrees. Lightly grease an 11-by-7-inch baking pan.
Step 2In a medium bowl, whisk together the flour, baking powder and salt. Set aside.
Step 3In the bowl of a stand mixer using the paddle attachment, or in a large bowl using a hand mixer, cream together the butter and sugar until light and fluffy, 3 to 5 minutes. Beat in the eggs, one at a time, then the vanilla. Slowly beat in the flour mixture.
Step 4Spread three-fourths of the batter into the pan, spreading the batter a little thinner in the center of the pan. Spoon the jam on top. Spoon the remaining batter over the jam in random dollops so the jam can still be seen.
Step 5Bake the bars until golden-brown on top and firm to the touch, about 40 minutes, rotating halfway through for even baking.
Step 6Cool completely on a rack before slicing.


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Merry Christmas to all and
to all a fabulous 09!
MLO has never particularly liked spicy food.
It has become an easy way to warn her that she might not like food.
We went to a party and they had some fire pits in their backyard as well as a fire in their fireplace.
We were telling her it was hot and she shouldn't touch it and she immediately said, "oh, the fire is spicy!"
Yes, exactly.
Labels: family
On the way to daycare I get gas sometimes.
I told MLO that the car was hungry and needed some food.
Almost every morning now she asks me if the car is hungry. Sometimes it is and sometimes it isn't.
One day on the weekend, hubby, MLO and I were on our way to the market and I told him we needed to get some gas.
MLO pipes up in the backseat: "Yeah daddy, the car is hungry and needs gas. I am hungry and need nuts." Upon which she promptly pops a nut in her mouth.
I love laughing with my little one.
My daughter is now called "Princess MLO".
She wears all her princess clothes whenever she can until I classify it as very dirty.
She knows almost all the various Disney princess songs and we sing them in the car.
She wants everything we buy her to have Disney princess on them somewhere.
She loves sparkly jewelry because princesses wear them.
She only wears dresses because princesses wear them.
She is a very benevolent princess luckily, she thinks she is supposed to kiss other people's hand and bow to them.
It is just her name that has to include "Princess" in front of it.
Oh and when she isn't a Princess she is "awesome MLO" because daddy told her she was awesome MANY months ago.
She is all of the above and more...
Labels: child, entertainment
It all went very well, we love our house and I am happy to have everyone over. It has some great entertainment features which make it a good choice (and therefore has been the choice) for our family parties.
Last year I cheated on the Turkey and got it pre-cooked so I just had to warm it up.
This year we had a little tighter budget, so I got to do all the hard work. Well sort of, I got to call my mother-in-law and have her come help me so it wasn't too hard. We cooked for 8 people so did a 16 pound bird, which turned out to be just perfect (we were expecting more left-overs).
We got compliments on it being the best Turkey ever, so we must have done ok.
MLO loved sitting at the table and having dinner with all her favorite people in the world. Her favorite was the corn my mother made for a side (I made people bring food so I didn't have to do everything), she had about three helpings of it.
Through-out dinner she kept pointing at people and saying how happy she was they were there. After that she just kept naming who was sitting next to who, over and over again.
In between dinner and dessert she went outside with my brother and they played "soccer" for a little bit. It was hilarious to watch because my brother was tired and very full so he ran sort of funny.
We were all up late talking and MLO didn't want to go to bed, even after everyone was gone she wanted to keep getting and giving hugs.


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There are a total of 7 luxury cottages at the Shining Waters cottage cluster. Six of these units are two storey 3 bedroom cottages and one is a single storey 2 bedroom unit. These cottages are all of exceptional quality and feature hardwood floors, whirlpool tubs and decks with propane BBQ's.
The 3 bedroom cottages have a king bed, double bed and 2 twin beds and fireplaces. The 2 bedroom cottage has a queen bed and twin bed in each bedroom. All cottages have satellite TV, air conditioning and high speed wireless internet. For 2017 we have upgraded to 39inch flat screen televisions.
All of these cottages are rated at 4 stars and they are some of the finer rental cottages available on Prince Edward Island.


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by Kat Martin.
Zebra paperback, 354 pages. 2016.
Contemporary. Seattle/Dallas/New Orleans.
"She met his dark-eyed stare. 'I get it, Ethan. I get that you don't trust women. That you don't trust me. I need to know . . . last night? Was I just a conquest? A notch on your bedpost? Because the way you make love tells me you've known a lot of women. Was I just one more?'
Something wild and unsettling moved over his features. He was in front of her in a few long strides, pulling her into his arms, holding her tightly against him.
'I'm sorry. Jesus, that woman makes me crazy. You weren't a conquest. I've never thought of you that way.' He tipped her chin up, returning her gaze to his. 'I swear it, Valerie.'
Her eyes burned. She read the truth in his face and she believed him. She almost wished she didn't. It would be easier to pretend last night had meant nothing to either one of them. That she was in no emotional danger, that her heart would be safe from Ethan.
He kissed her very softly, sinking in for a moment, letting her feel the heat, before he eased away. 'Allison Winfield had nothing to do with what happened between us last night. She never will.'
Relief and something deeper filled her chest. She managed to nod. 'Okay.'"
Ethan Brodie is on a new case, along with his buddy Dirk. They are in charge of protecting the girls doing a lingerie show that will appear in numerous big cities. All the girls have received a threatening letter. After the top model is found dead – murdered – security steps up. Ethan is the protection for the next lead girl, Val and Dirk cover her best friend. No clues or tips are found while in San Francisco, but when they move on to their next city, another death occurs with similar clues, only this time to a call girl. Perplexed and busy, they dig deeper into clues, spend most of their time checking on facts and asking questions. All the while – both Ethan and Dirk try to handle their strong emotions towards the women. And the women are doing the same.
Another big, big hit for Martin!! The security business keeps expanding and more fantastic hunks come on board. This had more than a murder and a love story to commend it. It's interesting being "backstage" in the lingerie business. And, of course, both Ethan and Val are exquisite examples of all that is dreamy and sexy.


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KBND Morning News
6:00am - 9:00am
Listen Live
2013-03-04 05:08:00
KBND News Sources
Oregon Congressman Earl Blumenauer says it's time to legalize recreational use of marijuana. He says millions of dollars are lost by law enforcement agencies when they are forced to prosecute marijuana crimes. He continues his efforts in Washington D.C. to move legislation that would take marijuana off the list of bad drugs and move it to the list of drugs that are okay when used responsibly, like alcohol.
Home | Public File | EEO | Employment Opportunities | Advertising Information | Links | Contact


text: Genuine early fifties couple looking for guy over 35 years of age for some good clean Adult Fun. You must be able to host as we have family at home . She is size 10 5 feet tall clean shaved pussy and very very sexy. Not looking for a guy over 6 inches hard . If you fit the bill get in touch .Pic would help but need at least to know you fit the bill. Condom a must .No anal or weird sex just good old fashioned sex.


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In an administration proud of its deregulatory approach, FDA Commissioner Scott Gottlieb has defied the trend. He's proposed to mandate lower nicotine levels in cigarettes and suggested a willingness to crack down on electronic cigarette products popular with kids. Matthew L. Myers, president of the Campaign for Tobacco-Free Kids, and CQ health reporter Andrew Siddons assess the proposals and the likelihood Gottlieb will follow through.
Show Notes:
- E-Cigarette Regulations Could Stymie Small Shops
- FDA Threatens to Stop Sales of Flavored E-Cigarettes
Get breaking news alerts and more from Roll Call on your iPhone or your Android.


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Who Just Got Flowers Delivered from Carly Rae Jepsen? This Girl! [VIDEO]
Not going to lie, I was having a pretty crappy day considering I fell at Walmart in some mystery substance that bleached my pants, but then, I got a special delivery. Flowers from Carly Rae Jepsen! How cool is that?!? Surely this means we're totally BFF now, right? Right!