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Russian Version
Moscow Annual Oil and Gas Conferences
oil-and-gas industry, electric power industry, coal mining industry
Phone: (495) 788-72-79, 514-58-56, 514-44-68;
Oil and Gas
Field Services
in Russia
2008 Summary
about the conference
Upcoming Events
Oil and Gas Industry
Supply Chain
2019 Summary
All summaries
Oil and Gas Industry
Supply Chain
2019 Summary
Oil and Gas Industry
Supply Chain
2018 Summary
Construction in Oil and Gas Industry
may 31, 2018
Modernization of oil and gas refinery
Supply Chain
2018 Summary
Oil and Gas Field Services in Russia
October 17, 2018
Offshore Oil and Gas Contracts
2018 Summary
Oil and Gas Industry
Supply Chain
2017 Summary
Construction in Oil and Gas Industry
2017 Summary
2017 Summary
Oil and Gas Field Services in Russia
2017 Summary
Offshore Oil and Gas Contracts
December 7, 2017
2016 Summary
2016 Summary
2016 Summary
2016 Summary
2016 Summary
Oil and Gas Industry
Supply Chain
2015 Summary
2015 Summary
2015 Summary
2015 Summary
2015 Summary
Oil and Gas Industry
Supply Chain
2013 Summary
Construction in Oil and Gas Industry
Supply Chain
May 28, 2013
Modernization of oil and gas refinery
2013 Summary
NEFTEGAZSHELF–2013 Summary
Oil and Gas Industry
Supply Chain
2012 Summary
Construction in Oil and Gas Industry
Supply Chain
May 22, 2012
2012 Summary
NEFTEGAZSHELF–2012 Summary
Oil and Gas Industry
Supply Chain
2011 Summary
in the Oil and Gas Industry
2011 Summary
Modernization of oil and gas refining facilities 2011 Summary
2011 Summary
Oil and Gas Industry
Supply Chain
2010 Summary
2010 Summary
Oil and Gas Industry
Supply Chain
2009 Summary
Drilling Equipment
2009 Summary
Oil and Gas
Field Services
in Russia
2008 Summary
Oil and Gas Equipment
Standardization 2008 Summary
Oil and Gas Industry
Supply Chain
2008 Summary
Offshore Drilling Equipment
2007 Summary
Oil and Gas Field Services 2006 Summary
SIBNEFTEMASH-2007, Summary
Gazprom meeting
Oil and Gas Industry
Supply Chain
2007 Summary
Oil and Gas Industry
Supply Chain
2006 Summary
Oil and Gas Field Services 2006 Summary
Offshore Field Equipment Conference Summary
How to Become Gazprom's Supplier Summary
Oil and Gas Field Services in Russia:
October 21, 2008
About the Conference
The third international conference "Oil and Gas Field Services in Russia" (NEFTEGAZSERVIS-2008) took place on October 21 in Radisson Slavyanskaya Hotel. At the annual meeting, heads of major oilfield services providing companies discuss the current market situation and performance results for the year. The October 21 meeting was attended by general director of Siberian Service Company Rafael Nnuriev, general director of TNG-Group Rinat Kharisov, general director of Rimera Group Vladimir Khristenko, operations vice-president of BK "Eurasia" Vadim Bayanov, chairman of the board of directors of OJSC "NPP "Burservis" Robert Ashimov, director of "Tyumenpromgeofizika" Dmitry Kryuchkov, general director of OJSC "Yakutskgeofizika" Alexander Makhorin, president of CJSC "Yamalpromgeofizika" Viktor Gapetchenko, president of OJSC "OTO-Management" Sergey Mishin, director of customer relations of Schlumberger Yury Pankov, vice-president drilling and well survey of Baker Hughes Drilling Dmitry Kuzovenkov, general director of OJSC "VNIPIvzryvgeofizika" Mustafa Arislanov, director of OJSC "Purgeofizika" Akif Asterov and many other players of the oilfield services market.
NEFTEGAZSERVIS-2008 was traditionally chaired by president of Integra Group Felix Lyubashevsky who initiated the meetings. The chairman said in his opening speech that "the year to come will be hard for the oilfield services market. Only strong and large companies able to invest in development will survive".
Prior to the conference, president of BK "Eurasia" Alexander Bogachev said in the interview to the n-g-k studio that experts predict a 20-25 percent fall in the oilfield services market. The crisis and anticrisis measures were discussed by many speakers. A speech was also delivered by Jane Kitson, commercial attachй of the U.S. Embassy.
Participants of Conference «Neftegazservis-2008»:
• Felix Lyubashevsky, President of ZAO "Integra Management"
• Rafael Nuriev, Director General of ZAO "Siberia Service Comapny"
• Rinat Kharisov, Director General of LLC "TNG-Group"
• Vladimir Khristenko, Director General of ZAO "Rimera"
• Yuri Ikonnikov, Head of Oil and Gas Production department of ОАО "NK "LUKOIL"
• Alexander Zakharov, Vice-president for well Workover OOO "Drilling Company Eurasia"
• Vadim Bayanov, Vice-president of production "Drilling Company Eurasia"
• Donat Folkmar Chief Representative of Bentec GmbH Drilling & Oilfield Systems
• Yuri Pankov, Representative Director of Schlumberger Lodgelco Inc
• Roman Onokalo, Manager in Russia and CIS countries, Sondex
• Dmitry Kuzovenkov, Vice president for well drilling and surveying Baker Hughes B.V.
• Jane Kittson, Commercial Attache of US Embassy
• Jan Januar, Director of Corporation Filia "Input/Output, Ink"
• Ivan Vorobyev, Cosalting Director, Head of practice "Effective staff management", EKOPSI Consalting
• Filarid Akhmatdinov, Director General of OOO "Bashgeoproekt"
• Robert Ashimov, Chairman of Board of Directors of OAO "NPP "Burservis"
• Nail Bilyalov, Drilling Director of ZAO "GEOTECK Holding"
• Nikolay Pekarnikov, Director General of OAO "Oil pump Service
• Natalya Andreeva, Member of board of Director of OAO "NizhnevartovskNIPIneft"
• Igor Malykhin, Operation Director of OOO "Mirriko management"
• Viktor Pankratyev, Deputy Director General of ZAO "NEGAS"
• Salavat Kuzyaev, Director General of OOO "Nekko"
• Akiff Askerov, Managing Director of OAO "Pugeophysica"
•Vladimir Kozlov, Chairman of Board of Directors of OAO "Yakutskgeophysica"
• Victor Gapetchenko, President of ZAO "Yamalpromgeophysica"
• Andrey Zhuravlyev, Technical Manager of Moscow Representative office LLC "Newco Well Service" • Alexander Radov, Executive Director of LLC "Newco Well Service"
• David Wilbeck, Business Manager od LLC "Newco Well Service"
• Evgeny Kolesnik, Head of geology department of OJSC "Oil Technology Overseas"
• Radif Tuktarov, Vice president for drilling of NJSC "Oil Technology Overseas"
• Sergey Gordeyev, Director General of OAO "Zavodoukovsky mashinostroitelny zavod"
• Sergey Babak, Service Department Director of OAO "AK OZNA"
• Valentin Maslov, Director General of ZAO "Service Center-Drilling Technology"
•Anatoly Dmitriev, Chief Machanic of OAO "Tatneft"
• Vladimir Shabrov, Vice president for strategy and development of OOO "Drilling Company "Eurasia"
•Fanzat Ismagilov, Director of Workover and enhanced oil recovery of OAO "Tatneft"
• Radik Afletonov, Director of OOO "Tatneft –Energoservis"
• Marat Timerkaev, Director General of OOO "Tekhinformservis"
• Sergey Denisenko, Deputy Director General of ZAO "TD "TMK"
• Alexander Makhorin, Director General of OAO "Yakutskgeophysica"
• Evgeniy Pechersky, Head of Oil and Gas Department of Schneider Electric
• Anton Finogentov, Marketing Director of OAO "AK OZNA"
•Marat Dautov, Director General of OAO "NPP Burservis"
• Rafail Khaerlanamov, Head of Drilling Department of OAO "NPP "Burservis"
• Konstantin Smirnov, Investment Director of Representative office of Limited Liability Company "Baring Vostok Capital Partners Limited"
• Vladimir Kornilov, Director General of ZAO "Volgar-1"
•Alexey Sokolov, Chief Engineer of ZAO "Volgar-1"
• Tatyana Bush, Director of OOO "STK GEOSTAR"
• Ivan Azamnov, Deputy Director of Technical Department of OOO "STK GEOSTAR"
• Mikhail Bobrov, Commercial Director of OOO "VNIIBT-Burovoy instrument"
•Irina Bondarenko, Marketing Specialist of SDT ZAO " ChTPZ – Complex piping systems"
• Dmitry Vasykov, Corporate Planning Director of ZAO "Siberia Service Company"
• Dmitry Borisov, Director for marketing and business development of ZAO "Integra Management"
• Victor Videneev, Director General of OOO "Engineering Company "Oil and Gas Service Systems"
• Andrey Pavlov, Executive Director of OOO "A.D.D Service"
• Bayazit Gabdullin, Deputy Director of OOO "NPF "Packer
• Alexandra Gennadievna, Marketing and Strategy Directorm of Smith Drilling Services
• Dzhamil Girfanov, Executive Director of OOO "Tatneft- AktubinskRemServis"
• Ologa Efremenko, Head of Marketing department of OOO "A.D.D. Servis"
• Bogdan Zaslabsky, Chief Engineer of ZAO PGO "Tyumenpromgeophysica"
• Alexey Valeev, Deputy Director General of Moscow Representative Office FGUP "Voronezh mechanical plant"
• Konstantin Kayurov, Director General of ZAO NPP GA " Luch"
• Svetlana Kononova, Manager of "Main line Equipment" Department of ZAO "Rimera"
• Denis Krylov, Deputy Director General for business planning and property management of OOO RN –Servis"
• Ildar Mannapov, Executive Director of OOO "Tatneft-AznakaevskRemServis"
• Alexander Merkulov, Deputy Director General of OAO "VNIIPIvzryvgeophysica"
•Artur Mosheev, Vice-president, Director of credit operations of OAO "NOMOS –BANK"
• Marat Nagumanov, Directoe of OOO "NPF "Packer"
• Magomed Omarov, Director General of OOO TD "RINAKO"
• Dmitry Osadchenko, Deputy General Director of OAO "Vilzhsy diesel after Mamynikh"
• Mikhail Margolin, PR Manager of OOO "Mirriko management"
• Mariya Patrusheva, Coroporate Communication Manager of ZAO "Rimera"
• Rustam Rakhmatullin, Vice-president, Commercial Director of ZAO "GEOTECK Holding"
•Evgeniy Boldyrev, Director of Service Technology of OOO "OTO Recovery"
• Andrey Sokolov, Chiev Engineer of ZAO "Volgar-1"
• Yuriy Sterlyadev, Chief Engineer of UK OOO "Tatneft- RemServis"
• Alexander Orlov, Deputy Director of Merging Department and strategic consulting of OAO "NOMOS-BANK"
• Yana Shorokhova, Director General of OOO "Rusta-Broker"
• Denis Tikhomirov, Production Director of OOO "SGK-Bureniye"
•Rustem Khabibullin, Chief Technologist of OOO NPP "Burintekh
• Yalik Yanbukhtin, sales Manager of OOO NPP"Burintekh"
• Petr Vanyukov, Regional sales Manager of OAO "GLAVLISING"
• Alexander Parshin, Deputy Director General for marketing and commerce of OAO "Zavodoukovsky mashinostroitelny zavod"
• Yuri Kreschenov, Service Sales manger of OAO "ZVEZDA-ENERGETIKA"
• Viktor Mikhaylov, Service Director of OAO "ZVEZDA-ENERGETIKA"
• Igor Vorobyev, Marketing Director for oil and gas equipment of ZAO "OKB Zenit"
• Evgeniy Tokmakov, Director for oil and gas equipment of ZAO "OKB Zenit"
• Mikhail Epov, Director of Institute of Oil and Gas Geology and Geophysics after A.A. Trofimuk" SO RAN
• Mikhail Loyferman, Member of Board of Directors of ZAO "IOMZ"
• Sergey Shteynikov, Director General of ZAO "IOMZ"
• Sergey Kipaykin, Deputy Director General for Sales of ZAO "KAMTENT"
• Viktor Skopenko, Deputy Marketing Director of ZAO "KAMTENT"
• Mikhail Panfilov, Executive Director of ZAO "Karbookam"
• Vasiliy Schukin, Vice president of OOO "L-Bureniye"
• Eduard Gura, Deputy Production Director of OAO "Mashprom"
• Alexander Tyrsin, Diretor General of OAO "Mashprom"
• Rustam Kamaletdinov, Deputy Director General of OAO "Oil pump Service"
• Gulnara Keingebulatova, Commercial Specialist of US Embassy
•Marat Bagautdinov, Deputy Marketing Director od OOO "SNEMA-SERVIS"
• Alexey Avraskin, Deputy Director of OOO "Region"
• Roman Kuzyakin, General Director of OOO "RIK-Servis"
• Viktor Kemkin, Deputy Director General for foreign economic projects of OOO Managing Company "North Expedition"
• Sergey Stepanov, Deputy Director General for drilling OOO Managing Company ""North Expedition"
• Dmitry Baldenko, Chief Research Officer of NPO "Burovaya Tekhnika"
• Yuri Baratynskiy, Head ofMarketing Department of ZAO "TD "TMK"
•Nadezhda Choporova, Supply Manager, OOO "Siberia leasing company"
• Alexey Shamilyev, Supply Chain Manager OOO "Siberia leasing company"
• Oleg Anashkin, Association of oil and gas manufacturers in Russia
• Aleksey Lavrov, Director General of OOO "Trans-Technology"
• Tduard Golubchenko, Marketing Department Manager of ZAO PGO "Tyumenptomgeophysica"
• Igor Kozulya, Marketing Director of ZAO "ChTPZ- Complex piping systems"
•Alexander Grishin, Deputy Director General of OAO NPP "Etalon"
• Nikolay Svetashov, Deputy Director General of OOO "Yugson Servis"
^ Up
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Phone: (495) 788-72-79, 514-44-68, 514-58-56;
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all of shimmer liquid luminizers. these beautiful liquid highlighters are a must-have for any makeup bag. make the most of them with these multi-tasking secrets....
1. mix all over shimmer with your favorite lip and cheek stain. this is a subtle, natural way to add shimmer to your cheeks without looking overdone.
2. use all over shimmer to highlight your eyes. dab the liquid on the ball of your eyes and the inner well to make them look bigger.
3. add a drop of all over shimmer to your favorite foundation. this is an easy way to transform your foundation and make your skin glow.
4. mix all over shimmer with your body lotion to make your skin look smoother and sexier for a special occasion. this trick works wonders on the red carpet :)
5. apply all over shimmer on the cheekbones with a foundation brush to enhance your features and slim your face.
happy highlighting! xoxo, stila
2 Responses to 5 things to do with all over shimmer
All of shimmer liquid luminizers can be used along with any other product applied to your eyelids, cheeks, lips, and all the other parts of the body good for applying makeup on. It helps make your skin glow during special occasions to make it look sexier and elegant. There are numerous ways of using the product including the ones mentioned in the article.
thanks! great tip :)
our faves!
stila cosmetics © 2010-2012. Powered by Blogger.

subset: commoncrawl


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WASHINGTON – The Catholic University of America men's cross country team competed at the Landmark Conference Championships in Bethlehem, Pa., on Saturday as sophomore Patrick Jones finished 20th overall as the Cardinals placed sixth.
Jones made the top-20 with a time 27:30.14, holding off Juniata's Evan Ulrich by 00:00.78 seconds and Susquehanna's Justin Skavery by 00:00.84 seconds.
Rookie runner Joseph LaPointe had a strong showing in his first championship race, placing 44th overall with a time of 28:34.6. Sophomore Peter Binck also posted a top-50 time at 28:53.2.
Sophomore Kevin Kuchler was unable to compete due to injury.
All Cardinal fans are encouraged to follow all of Catholic Athletics and sign up for "email-alerts" that sends the latest news directly to your phone, tablet or device. Also, be sure to follow the Cardinals on Twitter, Instagram and Facebook!


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What You Must Know About Online Casinos
Online casinos are for playing real cash by means of the web. They're sometimes called virtual casinos or internet casinos. There are a couple of on-line casino websites which have earned the trust of their clients. There are two types of on-line casinos established on their interface: Playable immediately from the internet site and playable with the online casino program. For the first sort of playing, you must not download anything. You could play instantly from the casino internet site. Lots of the internet sites require the modern variation of plug-ins like Macromedia Flash, Macromedia Shockwave, or Java built-in along with your web browser. For the second sort of playing, you have to download and set up the software furnished by way of the casino. The second kind offers faster game play than the first one.
Signing up is mandatory
To play in an internet casino, you must open an account first. Within the signup system you have to fill-up a kind along with your personal know-how, bank card no, a sum of money you wish to have to gamble etc. For enjoying online, you will be asked to install the plug-ins provided via the online casino like the popular topslotsite.com which has been used by many online gamers. The money you deposited or won will likely be saved on your account in models of credit score or digital money. Worldwide bank cards like PayPal, Visa, and MasterCard etc. Can be required to withdraw or deposit the money from or into your online casino account.
Some playing sites which give exclusive offers for your pleasure use program for the dealing and a few use actual dealers. For the software from dealing the successful percentile of the natural gaming is maintained. And the casinos which use actual dealer more often than not use a studio and provide a video feed of the dealing. The interfaces of probably the most preferred on-line casinos are thoroughly generated to produce the atmosphere of typical casinos in order that the gamer can feel the real thrill of playing.


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Q1 Building Goldcoast
Truly inspirational architecture is reflected in the world's tallest residential building. With its stunning glass encased structure, Q1 is the most striking urban designed building ever to appear on the Gold Coast skyline. Magnificent beach location, panoramic views from the surf to the hinterland and beyond.
Products - Xenian Big Balls (Moonlight) in floating version to a Contemporary Water Feature


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The origin of baseball is somewhat clouded, but one of America's favorite sports might have started in England with a game called rounders. Old pictures exist that show men standing on bases playing the game of rounders. Since the early days of the game, baseball has had a rich and fascinating history that intertwines throughout America's history. The love of this sport transcends gender, social status, and race, binding America together in its love of all things baseball.
Baseball History


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Sunday, October 18, 2009
Hot Promo Action
The Mary Onettes - Dare
The Mary Onettes - Puzzles
If you are a fan of British alternative pop of the '80s, then Sweden's Mary Onettes will no doubt resonate. I really dug their self titled debut from a couple of years ago - very moody, very New Order-ish. You will hear elements of a lot of the era's other key players - The Cure, The Smiths and Echo & the Bunnymen - in their songs. Their new album "Islands" is out next month. Good stuff.
Yura Yura Teikoku - Dekinai
DFA is releasing the new album by Japanese psych-rock legends Yura Yura Teikoku. Hollow Me is the 10th album of their 20 year career, and continues their unique spin on all forms of FM & AM classic rock music - from T-Rex to ESG to Can to 10CC. Hollow Me/Beautiful EP is out on DFA Records on November 3.
The Rakes - That's The Reason
The Rakes are set to release their third album 'Klang', digitally on October 20th in the US. I am a big fan of their debut 'Capture/Release' from 2005. I like angular post-punk and they continue to deliver.
Free Energy - Something In Common
Touring with The Rakes this fall is another DFA band, Free Energy, who deliver glammy '70s classic rock with more cowbell. Their debut album Stuck On Nothing, produced by James Murphy, will be released January 26 on Astralwerks/DFA Records.
Neon Indian - Terminally Chill
Neon Indian is Alan Palomo. He combines equal parts synthpop nostalgia, dream pop lullabies and grinding guitar noise, the result of field recordings, record samples and a collection of bizarre synth sounds. The album Psychic Chasms is out now on Lefse Records.
Old Wives Tale - Amphetamine
Old Wives Tale - Royal Fresh
Miami duo Old Wives' Tale is brothers Jaime & Felipe Valencia who combine their contrasting music personalities to create a sound composed of distorted bass lines, disco beats and twitchy guitars. They also like more cowbell. Michel Gondry liked them enough he directed the video for Amphetamine. Their album Younger Limbs is out now on The Creative Motel, Inc.
Bear In Heaven - Lovesick Teenagers
Bear In Heaven - Wholeheartedness
Space rockers Bear In Heaven's second full-length Beast Rest Forth Mouth is out now on Hometapes. They combine krautrock grooves and shimmery synths with classic 70's psych and prog rock. Very tasty.
Posted by Michael at 3:15 PM
Labels: hot promo action
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For Love of the GameDVD - 2000
From the critics
Community Activity
CommentAdd a Comment
To much swearing, way to much swearing.
This is a great movie and a great Baseball movie. I have watched it many times.
not sure why this movie didn't get more acclaims. Both Kevin Costner and Kelly Preston go all out for an outstanding performance. rom-com along with "live" baseball, just like a regular broadcast. Check it out.
You'll be presently surprised
What's better to watch than "America's Game" - on America's Independence day?! FOR LOVE OF THE GAME is an old-fashioned Hollywood sports story/romance with Costner turning full-circle from his BULL DURHAM performance and actually falling in love - and getting the girl in the end. It's not FIELD OF DREAMS - but I'm a sentimental slob for a good 'boy-meets-girl' story and the baseball background resonates for me, along with the new social relevance: He's a loner with an edge - she's a single mom with a career and a good but challenging teenage-daughter.
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Young Essex Assembly
Huge congratulations to Illiana Anderson 8N, Matthew Austin 8U and Jamahl Godward 7N who have been elected to the Young Essex Assembly. They will represent the views and interests of young people from the mid-Essex area and could be picked to join the UK's Youth Parliament. They were elected alongside 72 others from across Essex after a three-month campaign with 19,000 votes cast.


text: In 2007, an 18 ft. x 24 ft. building with an 8/12 roof pitch was moved to the site as the beginnings of a grain elevator. In the fall of 2009 we built a two-story addition, which measures 16 ft. x 24 ft. We covered it with 1 ¼ in. corrugated galvanized steel to make it period appropriate. Once the building was complete we put down an oak tongue and groove floor, built an office area and added some machinery. We now have a loading dock on two sides of the building and line shafts in place to run the machinery to provide demonstrations typical of early small town elevators.


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To Capture the Attention of Decision-makers, Your Content Has to Go Deep
Content quality has improved significantly since Google changed their algorithm to reward quality. But while much of the B2B content out there now features good writing and nice design, it tends to be shallow. This content is ideal for those who are just beginning to explore a topic, but doesn't provide real value to those who are experienced.
That's a big …Read More
Work Smarter, Not Harder: Let Content Do the Work of Selling
Salespeople are a tenacious bunch. They send email after email, make call after call, meet with prospect after prospect, then get up morning after morning and repeat. Salespeople work hard. But hard work doesn't make sales — at least not efficiently.
Most B2B companies now understand the role that content plays in marketing, to generate leads. But many companies are still relying on "hard …Read More
Powerful Case Studies: Buying Decisions Are Made at the Intersection of Insight and Customer Stories
Everyone is drawn to a good story. Why? We love to imagine ourselves as the protagonist, overcoming the odds and winning. Stories are inspiring. Stories help us understand how the world works.
Ultimately, stories give us clues about how to solve our own problems in the real world. And stories are attractive because they're concrete. They're visual, easily pictured in the …Read More
Creative Communication is a Revenue Turbocharger
Scientists have debunked the myth that we only use 10% of our brains. But the myth is persistent because it's so believable. One of the worst offenders that makes this false statistic so convincing is marketing copy. Cliches invade websites and sales collateral like a particularly nasty insect species. Buzzwords and abstract phrases that mean nothing are everywhere, and they're …Read More
4 Steps to Improve Your Content Quality for Better Leads and More Conversions
Content marketing has grown up—nearly every size company in every industry is using content. As a result, there's a lot of content out there. But although much of that content features good writing and nice design, it tends to be shallow and similar to what's already been published. Which is why companies (and the marketing agencies creating content for them) …Read More
Improve Your B2B Lead Quality by Adding These 3 Elements to Your Content [INFOGRAPHIC]
Many B2B marketers are getting lots of "likes" and "shares" on their content, but they struggle to get leads to convert into paying customers. People genuinely appreciate their content—it's helpful, engaging, and interesting. But while engagement is important, it's not what B2B marketers ultimately need. 85% of B2B marketers say their top goal is lead generation, and 84% say it's …Read More
Use Case Studies to Shorten Sales Cycles and Convince Wary Prospects
Committing to an enterprise-level purchase is a stressful decision for most B2B buyers. There are real consequences to the business if a vendor performs badly, such as angry customers, lost revenue, or fines associated with failing to follow regulations. An individual's promotion (or job) could be on the line if things don't go as planned. Which is one reason big decisions tend to …Read More
Want to Win at B2B Content Marketing in 2017? Focus on These Fundamentals.
Marketing has gotten pretty complicated. There are so many strategies, infinite numbers of tools available, and myriad metrics to track. It can be overwhelming.
Marketers are getting so focused on the new and the changing that they're losing sight of the fundamental. But the reality is that the fundamentals are still fundamental and they haven't changed. And if you stay focused …Read More
Content Distribution Strategy is Overrated—Here's Why (Plus, 3 Ways to Get Better Content Marketing Results)
With an overwhelming number of new websites and social media platforms cropping up every day, and with the influence of those sites and platforms constantly changing, marketers are increasingly focused on distribution and promotion strategy: where to share their content and how to get the word out. This is especially true for the notoriously-hard-to-reach B2B market.
In a recent study by Altimeter, …Read More
Use Sensory Images in Your Marketing to Stand Out from Your Competition
Let's face reality. Your prospects' inboxes are overflowing, their social media feeds are filled with your competitors' updates, and the first three pages of their Google searches contain sites just as good as yours. "Visibility" is no longer enough. Everyone is so visible that they're all blending into one big bulk that might as well be invisible.
You are not just …Read More


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Salmon Fishing in the Yemen
I've put a lot of thought into it and I'm pretty sure Salmon Fishing in the Yemen is the most boring movie title I've ever read. Going into it, you can't help but hope it's not one of those titles that's spot on like Snakes on a Plane or Zombie Strippers. You hope it's a metaphor for something else that is perhaps a bit interesting, but it's not. Salmon Fishing in the Yemen is, at its core, about salmon fishing in the Yemen, yet it's not boring. It's actually kind of heartfelt. It's certainly no perfect movie and not good enough to be considered a surprising gem, but the performances are grand and its story is life-affirming. It won't blow you away, but it's worth a look.
The film follows Fred (Ewan McGregor), a fisheries expert who is approached one day by Harriet (Emily Blunt), a consultant whose boyfriend has just gone off to fight in the war. Along with a visionary Sheikh (Amr Waked), she wants to start a project that will bring the sport of fly fishing to the Afghanistan desert. To do this, they need a lot of money, manpower and even more luck, considering the area's aridity is unfit for such a project. So with the backing of the British government that is looking to shed some positivity on foreign relations, they embark on a plan that only has a minor chance of success.
When taken as a whole, Salmon Fishing in the Yemen is underwhelming. Looking back on it reveals many narrative problems and contrivances. But in the moment, individual scenes work brilliantly and it makes you feel good about what the people onscreen are trying to do. Despite its flaws, it's inspirational to watch these people from all different backgrounds come together to work toward a common goal. Such diversity is absent in most films and although such a simple fact certainly doesn't make this movie anything special, it's worth noting all the same.
What Salmon Fishing in the Yemen does best is develop relationships. Although it does rely too heavily on soapy, feel good dramatic tricks at times, you come to care about everyone you're watching. McGregor and Blunt, two terrific performers in their own right, craft a believable relationship that blossoms over time. At first, they're at odds, Blunt ever the optimist that they can pull the project off and McGregor a cynical man who thinks it has no shot, but eventually they spur a friendship. McGregor's character begins to find hope and passion for the project, which brings the two together in a sweet and charming way. Unfortunately, as is expected with nearly any movie these days, a man and woman can't simply be friends and a romance sparks between the two. This is precisely where the film begins to go downhill, not only due to the fact that it's not unlike every other movie romance you've ever seen, but also because this inevitably leads to forced drama late in the movie after a surprise plot twist involving Blunt's boyfriend.
The events that transpire in the film are grounded and simple—this is not a fast paced movie, to be sure—except for perhaps a couple of remarkably silly moments, including one where Fred saves the Sheikh's life by swinging his fishing reel towards an oncoming attacker and hooking him, forcing the gunshot to stray off course. It's moments like these that make the film so hard to love. To hear that many didn't like it much at all would even be understandable, but what can I say? It worked for me. It made me laugh, it moved me and it ended. In the end, that's what we go to the movies for and despite its problems, that's why Salmon Fishing in the Yemen is recommendable.
Salmon Fishing in the Yemen receives 3.5/5


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a pathetic little blog
Finally finding yourself in the program that will help get you to your final goal only to have to share the experience with a person so hateful it sucks any pleasure you might have in it.
I've dealt with abrasive people in my life, but who hasn't? What I've never dealt with is a person who goes out of his or her way to be abrasive. As in every move is the result of masterful calculation of passive-aggression. Audible sighs. Eye rolls. Arm flapping. Stomping. All from a 40-something student who will be entrusted with the health care of your loved ones in just 1.5 short years.
I'm at a loss for why I'm the recipient of this person's animosity. It doesn't really matter though because I'm in this class and won't be quitting no matter how much a dick this person chooses to be toward me. What I get is experience on how to deal with jerks, an extended lesson in diplomacy and how to set a good example of taking the high road to the younger students.
And that was Summer School Day 1. I think there was some skills practice in between all the shit slung my way.
This page is about Me.
This page is about "frectis".
This is my email address: email@example.com. Write to me. So I can feel like one of those popular bloggers.


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November, 7
Blink-182 announce new UK tour
Blink-182 will tour the UK in July.
The pop-punk band will return to the stage for a 10-date run of shows in 201,7 kicking off in Cardiff's Motorpoint Arena on July 3 and finishing up in London's O2 Arena on July 19.
The news follows the making of their seventh album 'California' which was released earlier this year; over two decades after their debut album 'Cheshire Cat'.
It's thought they'll delight their loyal following by playing a mix of fan favourite tracks like 'All The Small Things' and 'Miss You' and songs from their latest album when they take to the stage in towns across the UK, including Nottingham, Leeds, Birmingham, Newcastle, Glasgow, Manchester, Liverpool, Bournemouth.
'California' was written without their long-time guitarist Tom DeLonge - who has been replaced in the band by Matt Skiba of Alkaline - and speaking about the change in lineup in a new documentary, bassist Mark Hoppus revealed the musician was the only viable choice.
He said: "The only person we talked about coming and playing our shows was Matt. The reception to Matt was amazing, overwhelming. People were chanting his name before we even came out and played."
Blink- 182's 2017 UK tour dates are:
Cardiff, Motorpoint Arena (July 3)
Nottingham, Motorpoint Arena (July 4)
Leeds, First Direct Arena (July 5)
Birmingham, Barclaycard Arena (July 7)
Newcastle, Metro Radio Arena (July 9)
Glasgow, The SSE Hydro (July 11)
Manchester, Arena (July 14)
Liverpool, Echo Arena (July 15)
Bournemouth, BIC (July 17)
London, The O2 (July 19)
Source: www.msn.com
November, 7
November, 7
November, 7
November, 7
November, 7
November, 7
November, 7
November, 6
November, 6
November, 5


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Behind this unassuming storefront on Hoxton Street Market lies a very exciting creative space for young writers. Run by a group of passionate volunteers, the Ministry of Stories was founded in 2010 by Nick Hornby, Lucy Macnab and Ben Payne with the simple mission of helping kids become better writers and learn more about writing and reading.
I first heard about Ministry of Stories through a graphic design blog as a case study by a local small creative studio; they had done all of the graphics for the Ministry of Stories and its Monster Supplies (the storefront, which allows it to be in a commercial property), and I fell in love with what MoS is trying to do in a still underserved community.
So last year I went for a mentor training session, a full day affair that ended with a CRB form I had to take away and fill out to ensure I wasn't a child molester, reference forms for current and past jobs, and a lot of excitement about working with kids and helping them learn to love writing.
And then, I didn't do anything with it. A friend of mine pointed out to me on Saturday night that I did go and leave my job and start a new one and then train for and run a marathon, so you know, I was busy. But I always felt bad about not following through, so earlier this year I logged on to the MoS and found an open Saturday drop in session (most of their activities are during the work week, in the middle of the day, which is really unfortunate for me) and just signed up.
This past Saturday was the day and it was fantastic. 1.5 hours with 30 8-13 year olds is quite challenging, but also really rewarding. About half way through, I found myself with a difficult student and was really proud that I could get her to concentrate on a task and help her get some meaning from the session. It was also really amazing to see the dedication and ambition of some of the older kids, who come weekly with stories they have been working on for a while – one girl is writing a detective novel about a private eye, while another boy is writing a novel about a child who works in a factory and grows up to be Prime Minister. I was really impressed by them and their enthusiasm and optimism.


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The Grey Panthers
15 June 2016, - Redcar, Errington Woods & Marske
|Photographs of the walk are displayed as
"thumbnails", below. If you want to see a larger version, with commentary,simply
select the thumbnail. You may then view the larger images as if in a
slide show. For some details of the walk itself, select the Route


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I'm so excited to share my stop on the Love Notions Summer Lace Blog Tour! Be sure to check out the other awesome ladies if you haven't yet–they are all so inspiring!
My take on summer lace is an all-white, all-lace Luna cami. It's the perfect layering piece that I know will take me into the next season.
The best part about using lace for this cami is that it's so easy! No hemming, and the only way I could have made it easier would have been to use lingerie elastic for the straps.
I paired my lace Luna with a new floral Harmony blouse. The Harmony is my new favorite woven tee pattern, as it fits me wonderfully without major adjustments. The only change I made was a full bicep adjustment as well as adding a little extra length to the sleeve.
I lucked out and found both fabrics for this outfit on super Clearance at Hobby Lobby. I only paid $2 for just over a yard of lace–how crazy is that?!
That's it for me–nothing too earth shattering but definitely my new favorite wardrobe staple!
Again–don't forget to check out the other awesome ladies on the tour:
Monday: Sewing Curves SewSophieLynn Kainara Stitches
Tuesday: doodlenumber5 My Heart Will Sew On Third Shift Creations
Wednesday: Princesse et tresors Phat Quarters
Thursday: All Things Katy! Back40life,Sprouting JubeJube
Friday: Sew Like a Sloth Very Blissful Seamly Behaviour
What are you working on this summer?!


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Welcome to Lihue, HI! Lihue Hotels offers great rates on several preferred hotels near Lihue, Hawaii. All the hotels we offer have been approved by AAA and the Mobile Travel Guide, the authorities in hotel inspection. All hotels offer a generous savings off of regular hotel rack rates. Book securely online for great rates on hotels near Lihue!
Marriott Kauai Resort and Beach Club
Mere steps from the stunning shores of Kalapaki Bay, the Kauai Marriott Resort and Beach Club features a slew of recreational facilities, including a spa and a 26,000-square-foot pool, and is approximately one mile from Lihue Airport. Local attractions include Poipu Beach Park, approximately 15 miles away, and Waimea Canyon, located 43 miles from the resort. Each morning, guests of the Kauai Marriott Resort and Beach Club rise to find the crystal-clear waters of Kalapaki Bay beckoning to them. Down by the sea, guests may swim, catch some rays… more.
Marriott Kauai Resort and Beach Club
3610 Rice Street
Lihue, HI 96766 US
Kauai Palms Hotel
2931 Kalena Street
Lihue, HI, 96746
Kauai Shores, An Aqua Hotel
420 Papaloa Road
Kapaa, HI 96766
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414 Wailua Kai Street
Kapaa, HI 96766
Kauai Beach Resort
4331 Kauai Beach Dr
Lihue, HI 96766
Castle Kaha Lani
4460 Nehe Rd
Wailua (kauai), HI 96766
Plantation Hale Suites
525 Aleka Loop
Kapaa, HI 96746
Kauai Coast Resort
520 Aleka Loop
Wailua, HI 96746
...More Hotels
Lihue is the county seat of Kauai County, Hawaii. Lihue is located at 21°58'29" North, 159°21'56" West and is the second largest town on the Hawaiian island of Kauai with a population of 5,674 inhabitants. Once a sugar plantation city, Lihue has developed becoming nowadays a hub for traffic into and off of the island. Lihue is the first port of call in Kauai, being served by the Lihue Airport and by the seaport southeast of town at Nawiliwili Bay.
Lihue offers to its' visitors the advantage of being only a few minutes away from several good beaches. This city is a perfect blend of beauty and enchantment, still preserving its small town atmosphere. Lihue is surrounded by magnificent lagoons, cascading waterfalls and lush tropical settings. This city is home to a total of 139 mountain summits and peaks.
At the Kauai Museum tourists can admire an impressive collection of ancient Native Hawaiian artifacts such as poi pounders, pohaku, calabashes and tapa. Visitors can also take part in the events such as May Day Lei Day and the Christmas Craft Fair. Near Lihue is located The Ahukini State Recreation Pier a protected marine area and a very popular place for pole fishing and crab netting. This pier offers great views of the Hanama'ulu Bay.
At the Wailua River State Park visitors can enjoy hiking, kayak up the Wailua, water sky, ride a motor beat, explore, take photos, view the amazing scenery and interpret historic sites.
For the tourists interested in history and culture we recommend the Kilohana Plantation or one of Lihue's old churches. North of Lihue you can stop and admire the Wailua Falls, or if you want to avoid the crowds you can book a kayak guided tour around Kipu Falls and hike, swim, kayak up the Huleia River.


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- Research
- Open Access
The Cuban scorpion Rhopalurus junceus (Scorpiones, Buthidae): component variations in venom samples collected in different geographical areas
© Rodríguez-Ravelo et al.; licensee BioMed Central Ltd. 2013
- Received: 30 October 2012
- Accepted: 12 December 2012
- Published: 20 May 2013
The venom of the Cuban scorpion Rhopalurus junceus is poorly study from the point of view of their components at molecular level and the functions associated. The purpose of this article was to conduct a proteomic analysis of venom components from scorpions collected in different geographical areas of the country.
Venom from the blue scorpion, as it is called, was collected separately from specimens of five distinct Cuban towns (Moa, La Poa, Limonar, El Chote and Farallones) of the Nipe-Sagua-Baracoa mountain massif and fractionated by high performance liquid chromatography (HPLC); the molecular masses of each fraction were ascertained by mass spectrometry analysis. At least 153 different molecular mass components were identified among the five samples analyzed. Molecular masses varied from 466 to 19755 Da. Scorpion HPLC profiles differed among these different geographical locations and the predominant molecular masses of their components. The most evident differences are in the relative concentration of the venom components. The most abundant components presented molecular weights around 4 kDa, known to be K+-channel specific peptides, and 7 kDa, known to be Na+-channel specific peptides, but with small molecular weight differences. Approximately 30 peptides found in venom samples from the different geographical areas are identical, supporting the idea that they all probably belong to the same species, with some interpopulational variations. Differences were also found in the presence of phospholipase, found in venoms from the Poa area (molecular weights on the order of 14 to 19 kDa). The only ubiquitous enzyme identified in the venoms from all five localities studied (hyaluronidase) presented the same 45 kD molecular mass, identified by gel electrophoresis analysis.
The venom of these scorpions from different geographical areas seem to be similar, and are rich in peptides that have of the same molecular masses of the peptides purified from other scorpions that affect ion-channel functions.
- Cuba
- Hyaluronidase
- Peptides
- Phospholipase
- Proteomics
- Rhopalurus junceus
- Scorpion
Scorpion venoms are very diverse in chemical composition and action modes, but are known to contain enzymes, peptides, carbohydrates, lipids, biogenic amines and many other components [1–4]. Venom lethality among scorpions of the family Buthidae is attributed to the presence of toxic peptides that recognize ion channels, mainly Na+ and K+[5–7]. The most important peptides for toxicity are the alpha- and beta-scorpion toxins (here abbreviated α- and β-NaScTx), which are specific for Na+ channels. Two recent reviews on the subject can be found in Gurevitz [8] and Pedraza-Escalona and Possani [9].
Studies on the bioactive components of Cuban scorpions are scarce. As to the venom of Rhopalurus junceus (here thereafter abbreviated R. junceus), only a general biochemical and molecular biological characterization has been reported with some details [10]. The fact that this species is known to occur in different geographical locations in Cuba has instigated the present study.
Since venom contains a mixture of peptides and proteins secreted by specific glands, an analysis of venom components can produce a valuable fingerprint that is useful as a reference tool in taxonomic analysis, as a complementary method for morphological and behavioral characterization, but also for species identification and classification of related specimens [11].
Intraspecific venom variation has been previously studied in scorpions [12–15], and extensively in snakes [16, 17]. As well as being of interest in the study of the ecology and evolution of venomous animals, this phenomenon is relevant to therapy for scorpion stings and snakebites. Scorpion and snake venoms also represent a valuable natural source of biological products for basic research and biomedicine [16]. Thus it is useful to determine whether the components of interest are more abundant in the venom of certain individuals than those of others.
Venom composition variability among some scorpion species has been examined for such purposes as ascertaining measurable differences in the concentration of α-NaScTx between individual Tityus serrulatus scorpions of Brazil [18].
More recently, venom variability in specimens of the scorpion T. serrulatus was assessed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) analyses, by which significant variations were observed in the venom from a single individual extracted at different times, especially in later extraction events [13]. These variations are most likely related to production dynamics in venom gland cells. Equivalent results have been found in the venom of the scorpion Androctonus mauretanicus, whose lethality varies from specimen to specimen and pharmacokinetic parameters differ markedly in venom and antivenom [19].
Badhe et al. [20] detected intraspecific protein diversity using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in venom from the red scorpion Mesobuthus tamulus from western and southern India. The authors suggested that differences in the band patterns of separated proteins in all venom samples indicated the existence of genetic variation in venom production among scorpion strains of western and southern India. Omran and McVean [12] examined intraspecific variations in venom from the scorpion Leiurus quinquestriatus collected in Egypt (Sinai and Aswan Deserts). SDS-PAGE and a densitometric gel scan showed that venom originating from Aswan contains several protein bands that are absent from Sinai-sourced venom. In contrast Sinai venom appears to have a large proportion of protein in the molecular weight range known to include toxins.
Proteomic analyses show that single scorpion venom might contain more than 100 peptide components [21, 22]. Out of more than 1,500 different species of scorpions known to exist in the world, approximately 50 have been characterized either by proteins or individual peptides [5, 6, 23]. Approximations approach nearly 100,000 for distinct components in scorpion venom peptides [7]. An estimative from 2006 shows that no more than 350 components were known at that time [7]. However, updated information available in the Animal Toxin Annotation Program of UniProt (http://www.uniprot.org/program/Toxins) provided manually curated protein entries for approximately 750 scorpion venom proteins.
A number of hyaluronidases have been isolated from the venoms of bees, snakes, wasps, hornets, spiders and fishes [24–31]. Furthermore, hyaluronidases have also been purified from the venoms of a few scorpion species: Heterometrus fulvipes, Tityus serrulatus, Palamneus gravimanus and Buthus martensi[32–35]. The only hyaluronidase sequence information obtained from scorpion (Tityus stigmurus) venom indicates a lack of similarity to any other known protein sequences [36]. However, studies so far have focused on the low-molecular-weight toxins, probably due to their apparent medical/biological significance, whereas the high-molecular-weight toxins or proteins have been scarcely studied thus far.
The comparison of sufficiently representative venom variations among scorpions from different geographical areas might be useful for taxonomic differentiation, as was found between the American scorpions of the species Centruroides sculpturatus and Centruroides exilicauda, initially thought to be synonymous species [37]. The study conducted by the latter authors should be taken into consideration to ascertain whether the differences between individuals identified as a single species but collected in different geographical areas might support a different taxonomic assignation.
In relation to the venom of R. junceus, the scorpion of interest in the present communication, there are several reports dealing with its toxicity and pharmacology, electrophoretic and chromatographic separation, antimicrobial activity and general biochemical characterization [10, 38–43].
This scorpion species is equally distributed throughout Cuba, including the islands adjacent to the country. It is the most common scorpion in the country, and stings hundreds of people each year, but its venom contains an LD50 dose of about 8.0 mg/kg, which is not dangerous to humans (http://en.wikipedia.org/wiki/Rhopalurus_junceus). No deaths have been reported, with the exception of a few cases most likely due to anaphylactic shock following a hypersensitivity immune reaction [38].
Apart from its bioactive peptides, the venom also displays activities of such enzymes as hyaluronidase and phospholipase. While the hyaluronidase activity appears ubiquitous in all venom samples collected in distinct geographical areas showed this activity, phospholipase activity was observed in scorpions collected in the humid area of Baracoa, Guantanamo Province.
In the present communication, the venoms of scorpions collected in five different locations were studied after chromatographic separation and proteomic analysis. Despite the important morphological similarities found, the different biochemical and mass spectrometry results indicate that we might be dealing with very important interpopulational variations, or even with different subspecies of the R. junceus scorpion found in the five populations of the Nipe-Sagua-Baracoa mountain massif. Among a total of 227 molecular masses identified from the five different geographical areas studied, we identified 153 as different components.
Sources of venoms and chemicals
Scorpions of the species R. junceus were collected in five different locations, with official permission (CITMA 007.01.126) of the "Centro de Inspección y Control Ambiental" of Cuba for the collection and management of flora and fauna.
The R. junceus venom was obtained by electrical stimulation from ten adult scorpions from five different geographical areas of Cuba. From Moa: nine females and one male, from La Poa: six females and four males, in Limonar: seven females and three males, from El chote: eight females, two males, and in Farallones: seven females, three males.
After three days in captivity, the individual scorpions from each area were milked and pooled together, with the venom secretion having been collected inside small ampoules and lyophilized. The venom was kept at −20°C. Once in Mexico, the content of each tube was solubilized in water, centrifuged at 10,000 × g for 15 minutes; and the absorbance of the soluble venom was read at 280 nm. The soluble venom (assuming one unit of absorbance is equivalent to 1 mg/mL of venom concentration) was adjusted to a final concentration of about 2 mg protein in 300 μL, and applied to the HPLC column for each independent chromatographic separation. The solvents and chemicals used were of analytical grade and double-distilled water was used throughout [10, 21].
Isolation procedures
The soluble venom from each locality was separately applied into the high performance liquid chromatographic (HPLC) system. Usually, 2 mg dissolved in about 100 μL was applied each time. An analytical C18 reverse-phase column (dimensions of 250 × 10 mm) obtained from Vydac (USA) was used for HPLC fractionation. Components were purified using a linear gradient from 0% solution A [0.12% trifluoroacetic acid (TFA) in water] to 60% solution B (0.10% TFA in acetonitrile), run for 90 minutes. The detection was monitored by absorbance at 230 nm with 0.5-unit sensitivity and eluted at 1 mL/min flow-rate. Fractions were collected manually and dried using a Savant Speed-Vac drier.
Mass spectrometry analysis
For fingerprint analysis, the approximate amount of protein content of each dried sample was estimated, based on the HPLC profile of the separation (integral under the curve for each component). The samples were reconstituted to a final concentration of 0.1-0.5 μg/μL of 50% acetonitrile with 1% acetic acid and directly applied into a Finnigan LCQDuo ion trap mass spectrometer (USA), using a Surveyor MS syringe pump delivery system. The eluate at 10 μL/min was split to allow only 5% of the sample to enter the nanospray source (0.5 μL/min). LC-MS/MS analyses were performed with a PicoFrit needle/column RP C18 from New Objective (USA) using a fast gradient system from 0 to 60% of B (acetonitrile and acetic acid 1%) in 60% minutes. The spray voltage was set between 1.6 and 1.8 kV while the capillary temperature was set from 100 to 130°C depending on the chosen experimental conditions. All spectra were obtained in the positive-ion mode. The data acquisition and the deconvolution of data were performed with the Xcalibur software on a Windows NT PC system.
Hyaluronidase activity
The activity of the enzyme hyaluronidase was determined using two different methods described, respectively, by Tolksdorf et al. [44] and by Cevallos et al. [45]. The first protocol is known as the turbidimetric method, in which hyaluronic acid (0.4 mg/mL) is dissolved in 100 mM Na-phosphate buffer, pH 5.8, containing 150 mM NaCl and hexadecilpiridinium made at 10% concentration in distilled water. Small amounts (2–4 μg) of either venom or semi-purified fractions were used in each tube. The turbidity was read at 540 nm. The method by by Cevallos et al. [45] employed sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) [46], where the acrylamide was copolymerized with 0.5 mg/mL hyaluronic acid. The apparent molecular weight of the enzyme, if present, was observed by a clear degraded zone in the position of the SDS-PAGE gel where the enzyme had migrated, after staining and washing.
Phospholipase activity
Phospholipase activity of soluble venoms was determined by incubating aliquots of the samples into the gel-plate medium containing the appropriate substrate, as described by Habermann et al. [47]. This is a sensitive and very simple assay, consisting of a plate containing egg yolk (rich source of phospholipids) in a solid medium of 0.3% agarose in the presence of 10 mM calcium, and observation of the clear extension around the point at which the samples are applied, after a certain number of hours. For the entire venom, at least five-hour incubation is required. A known phospholipase is always used as positive control and buffer alone as negative control.
Results and discussion
The venoms do contain some closely related profiles in relation to the major components and time of elution, but the relative concentrations seem to be variable. This is already well documented from similar analyses conducted on other venoms, especially when the number of individuals used for preparation of the pooled mixture is small [48, 49].
During three decades, the work performed in our laboratory on venoms collected from scorpions of the species Centruroides noxius and Centruroides limpidus limpidus, collected by the thousands every year (usually four to five thousand annually), has always shown a very similar separation profile using Sephadex G-50 and Ion-exchange columns, also confirmed when using HPLC [50, 51]. Some of the differences reported herein might well be due to the number of specimens used, but also to the different number of males and females in a given sample.
It has been reported that some differences are found when scorpion venoms are fractionated separately for each sex of the same species [52, 53]. Nevertheless, it is evident that the majority of the venom components are eluted in 30 to 60 minutes, regardless of the sample origin. They do contain components that elute quite similarly in a C18 reverse-phase column. Peptides eluting around 30–40 minutes from other venoms of scorpions of the family Buthidae correspond to molecular masses close to 4 kDa, and have been demonstrated to be peptides that recognize K+ channels, whereas peptides eluting in 40 to 50 minutes (the most abundant components of the venom) usually correspond to a molecular weight of approximately 7 kDa and have been shown to be specific for Na+ channels [5, 6, 36].
These results are also in agreement with a previous communication from our laboratory dealing with the biochemical and molecular biological characterization of R. junceus venom [10]. In the latter publication the presence of 16 different peptides in the range of 7–9 kDa were cloned and reported, among which, are certainly the Na+-channel toxins. In the same publication, physiological experiments showed the presence of both types of peptides in this venom: those affecting Na+ and those affecting K+ channels, especially hERG potassium channels.
As shown in Figure 2, the number of subfractions separated according to geographic areas is on the order of 60, all of which were submitted to mass spectrometry analysis.
Retention times and molecular masses of venom components
RT (min)
Moa MW Da
La Poa MW Da
Limonar MW Da
El Chote MW Da
Farallones MW Da
4036, 3873, 4139, 4195, 3947, 4033
*4033, 4144
4837, 4943
*3384, 4940
*3875, 3983, 4143, 3945
*4835, 3963
*1922, 4010
*5208, 5769
4199, 4271, 4298, 4048
4271, 4198
6005, 6095
*3360, 4274
*4710, 4297, 4209, *4047, 6543
4199, 4297
*4048, 6544
7081, 7196
*7192, 7305
7087, *7163, 7053
6544, 7080, 7192
7342, 7101
7053, 7085
7037, 7148, 7103
7115, 4184, 4182
*7100, 7265, *7099, 7110
7371, 7431
*4181, 7430
*7101, 7375
*4183, 7542, *4871, 4597
12735, 7285, 7397, 9264
4467, *6366, 6946
3716, 8040
*6367, 6477, *7394, 7509
7064, 8045, 8200
7039, 7427, 7355
*7031, 7061, 7086, 8042, 7354
7032, 7061, 7090
10196, 12236, 14080, 14275, 17140
7355, 7115, *7136, 7079
7034, 7355, 8196
8199, *7136, 7079
7518, 7402
7128, 6934
*7040, 7313
7128, 7180
*7128, 7403
*8081, 7655, 7539
*7109, 7971
6993, 6891
6860, 13722
6994, 6861
*7109, 7947, 6993
6910, 6861
6926, 7162
*6625, 6994, 7959, 6924
14946, 15925, 16923, 18770, 19755
*7160, 6970, *7233, 7039
7264, 7280
*14465, 15894
7946, 7263
*7263, 7898
*6897, 7281
A small time variation can be verified by the identification of identical components found in venoms originating from different areas, which strongly supports the suspicion that the venoms are from the same, or a very closely related, scorpion species. Before analyzing specific examples, it is important to note that the equipment used for mass spectrometry determination (LCQDuo) has effective resolution at the level of one atomic mass unit. Thus, components that differ by about one mass unit can be considered identical.
It is also possible that two peptides with the same molecular masses have different amino acid compositions, and hence are not the same. However, since we are comparing results obtained from scorpions assumed to be of the same species on account of their morphological characteristics, it is very likely that those having the same molecular masses are in fact the same peptides.
Taken together, these observations indicate the presence of about 153 different peptides among the five venoms. It is worth noting that this figure was obtained by adding each of the different masses obtained from the five localities where scorpions were collected. That is, the actual number of distinct peptides found in individual areas is less than 153, out of the 227 total masses obtained.
Consider the following partial list of coincidences which make quite clear that many of the components found have the same molecular masses and come from scorpions of different areas. With an elution time of around 6.9 minutes, a peptide weighing 5360–5361 Da was found in Moa and Far. A peptide of 2173–2174 was found in Moa, Lim and Far with an elution time of around 9 minutes. The same 4033 Da peptide was found in Moa and Lim; another weighing 3944–3945 Da was found in Lim and Cho; one of 4835 Da was present in Moa and Far. Peptides of 4198–4199 Da were found in Moa, Lim and Cho. Peptides eluting in around 37 minutes with molecular masses of 4297–4298 Da were found in Poa, Lim and Cho. Peptides weighing 4047–4048 Da were obtained in Poa, Lim and Far. Peptides of 7080–7081 Da were in Moa, Poa and Lim. Peptides with molecular masses of 7100–7101 were found in Poa, Lim and Cho. Peptides with molecular masses between 4181 and 4283 Da were found in four locations: Poa, Lim, Cho and Far, eluting in about 41 minutes. Peptides of 7354–7355 Da were found in Poa, Lim and Cho. Peptides weighing 7128 were found in Poa, Lim and Far. Peptides of 6993 to 6994 Da were in Moa, Lim, Cho and Far. Peptides with 6924–6926 are in all venoms. Similarly, those having a molecular weight of around 7160–7162 were found in all venoms. Peptides weighing 7263 Da were in Moa, Lim and Cho; others between 7280 and 7281 Da were in Moa, Lim and Far, whereas peptides weighing 8088 to 8089 Da are in Moa, Cho and Far. We have not indicated here all coincidences, but it is quite clear that many of the components found have the same molecular masses and come from scorpions of different areas.
Phospholipase activity was verified by the egg yolk-agarose technique (data not shown) in fractions eluting at retention time between 45 and 51 minutes only for the venom obtained in Poa (see molecular masses of Poa in Table 1).
Morphological analyses also performed on specimens collected in the five aforementioned areas strongly support the conclusion that they all belong to the species R. junceus (manuscript in preparation).
Taken together, all these results suggest that the scorpions in these five different areas of Cuba are either from the same species or subspecies of R. junceus, with the variations already indicated (differences in sex ratio used for milking), or intraspecific variations present in venom of scorpions from other regions [12–15].
It is worth mentioning that the five geographical areas are situated at different altitudes while the mountains arrayed from east to west along the island form a physical barrier between the north and south that gives rise to a climate gradient, leading to more rain in the north in contrast to the semi-desert conditions in the south. There are also two rivers (Sagua in the north) and (Yumurí in the east). Limonar and Farallones are separated by a mountain (460 m) and a river (the Sagua). Farallones and Moa are closely situated, about 20 km apart, at respective altitudes of around 70 and 12 meters above sea level. Furthermore, whereas Farallones has rocky soil, La Moa presents a sedimentary landscape. Limonar is separated from Farallones and Moa by its higher altitude (460 m) and the fluvial river Sagua. El Chote and La Poa are more than 180 Km apart, and are also separated by the rivers Sabanalamar and Yumuri. In addition, El Chote is situated at a much higher altitude (630 m) than La Poa, which is at the sea level. Despite the contribution of these geographical barriers to the variations found in the scorpion venom components from these different areas, the samples are very likely from the same species.
However, the fact that Table 1 shows molecular-weight variations in the components from the different areas leaves the speciation question unresolved. This aspect must be confirmed by means of more appropriate genetic markers. Since the most abundant components are in the range expected for peptides that recognize K+ and Na+ channels, further efforts should be focused on sequencing and conducting specific physiological assays with these components.
This communication reports the results of the first proteomic analysis of components from the soluble venom of the Cuban scorpion Rhopalurus junceus collected in five different geographical locations of the country. At least 153 different molecular masses compounds were identified, among which are peptides with molecular weights in the order of 4 kDa, known to be K+-channel specific peptides, and 7 kDa, known to be Na+-channel specific peptides, confirming earlier findings by our group showing the presence of such peptides in this venom [10]. Approximately 30 peptides found in the venom samples from the different geographical areas are identical, supporting the idea that they all probably belong to the same species of scorpion, with some interpopulation variations. The enzyme hyaluronidase is ubiquitously present in all samples, whereas phospholipase activity was found only in scorpions collected in POA. The HPLC separation of the various samples showed similar profile but with some variations, especially in the relative amount of each component.
Ethical approval
The present study was approved by the Centro de Inspección y Control Ambiental of Cuba for the collection and management of flora and fauna (CITMA 007.01.126).
This work was supported in part by grant IN204110 from Dirección General de Asuntos del Personal Academico of the National Autonomous University of Mexico (to LDP). RR Rodriguez was a recipient of a scholarship from UNAM, within the joint-PhD program of the University of Havana and the National University of Mexico.
Authors' Affiliations
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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I'm not the owner of the GD28 yet, but may become soon :)
On the boat I'm looking at I can see a crack which goes along the edge of the deck (at the gunwale), right under the wooden bulwark (I can see crack in the bulwark scuppers). In one place fiberglass was removed and I can see some (rotted?) wood inside.
- Could please someone describe construction of the hull/deck joint on Great Danes?
- What do you think this will take to repair?
I can see myself removing bulwark (is it attached with screws?), removing some material with a router and then filling it with something. But I'd like to know what to expect before ripping the boat apart.
Hope to join your club soon.
Best regards,


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Onome Talk Am! > Intimacy > THE EX-FACTOR
Being in a relationship and doing everything to make it work is work enough but having to deal with a partner who hasn't got over his/her ex puts more constraints on an individual.
I know how difficult it may seem to get over an individual with whom you have shared lots of interesting memories but it is also not a good feeling when you put the new person in your life through the bitter phase of having to understand and deal with a partner who is still very much engrossed in his past, making him/her feel he is not worth your love and care even though he is the new person in your life.
Relationship is better appreciated and enjoyed when the two people involved are so much Into each other without any interference in form of a ghost from the past.
There is a reason why a relationship that didn't work is called a past relationship,let it be in the past.No one deserves the constant reminder and insecurity of knowing that the person they are dating is physically with them but his/her heart belongs to another.
I know emotions are involved,no one is judging you.We all understand that it is not so easy to let go of a person you've grown used to.But do not expect the new person In your life to always understand why you can't seem to get over your ex, I am sure you will feel worse if the tables were turned.
Never make the new person in your life feel like a rebound,it is not a good feeling.
If you still think about your ex when you do not intend to,if you constantly relieve happy memories with the person while forgetting the bad ones, you need to make a conscious effort to purge yourself of him/her.
If thoughts of him gets you angry,frustrated and agitated, you still have a lot of work to do towards letting go.
Always remember that if you subject yourself to a world of mental and emotional hostage by subconsciously holding on to the past, you loose out on having new and better things in life.Stop thinking of what could have been.
Though your past may have been your first true love there is a reason it didn't work with him,forget what folks say about finding true love once in a lifetime.You can get over it,other people in your position were able to move on,You can too.
To help in your journey of letting go,here are a few tips;
1.LOVE YOURSELF:No one can love you as much as you.If you love yourself,you will realise that ending it was the best decision.
2.STOP THINKING ABOUT WHAT COULD HAVE BEEN:I know you would have made some plans while the going was still good, it is over now, stop thinking about how beautiful those plans would have turned out,accept that is over and move on.
3.ACCEPT HE IS NOT THE ONE FOR YOU:Yes, you have conditioned your mind to thinking he is the only one for you and you believe no one else can love you as much as he did.You think you can't get what you guys shared with another person.That is a lie from the pit of hell,if he is right for you, you guys wouldn't have gone your separate ways.
4.STOP THE COMPARISONS:You will only hurt yourself more if you continue to compare your past with your present, stop looking at the pros and cons of your ex and the new person in your life.Quit wishing the new person in your life has same attributes as your ex .No two persons are the same, not even twins.
5.TALK ABOUT YOUR FEELINGS:It is at this point that you need close friends and family the most. Talk to them and you might just see one or two people who have been through what you are going through,their advice on moving on might just be what you need to regain your sanity.
You need to work towards moving on, love will always find you.It only depends on you, do you want to continue living in the shadow of your ex or do you want to experience a new phase of love again.Give yourself another chance to experience love again, you might just have a better experience than your past. In the words of F. Scott Fitzgerald;
There are all kinds of love in this world but never the same twice.
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1 Comment
After reading this.. i sighed..
Interesting write up, deep, thoughtful..
I can definitely identify with this, if am not the basis for it in the first place.. hmmm↓


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Clue: Reprove severely
We have 1 answer for the clue Reprove severely. See the results below.
Possible Answers:
Related Clues:
- Attack severely
- Excoriate
- Scorch
- Criticize, and then some
- Rake over the coals
- Tell off in no uncertain terms
- Blister
- Criticize harshly
- Attack with harsh criticism
- Critique harshly
Last Seen In:
- New York Times - March 05, 2001
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So what's next?
"In general people experience their present naively, as it were, without being able to form an estimate of its contents; they have first to put themselves at a distance from it – the present, that is to say, must have become the past – before it can yield points of vantage from which to judge the future. "
A Quote by Sigmund Freud relating about the future of oneself in conducting life.
I think everybody feels a bit differently as they detox. What's next is probably working on yourself and your recovery every day.
It's also important to think about how you intend to eat and live after the detox. It's what you do most of the time that has the biggest impact on your health and body shape, not what you do occasionally. A two week change in habits is hardly going to have a dramatic impact on your well-being. It's long-term lifestyle habits that have the strongest influence over your current body shape, and your health.
Try to use your detox as a time to increase your awareness of the way you eat, what you eat, how much you eat, and why you eat. Take a closer look at your activity levels, the amount of sleep you get, and how you deal with stress. Try to get a better picture of what's working and what isn't. Then you will be in the best position to do something about it—now and in the future.
The DOABLE detox
Detox. A little word with a lot of draw. Really it's not just an invitation of goodbye coffee, hello herbal tea, but the promise is vast. Just a week into a detox and you're likely to experience a boost in energy, clearer skin, less gas and bloating and even less congestion.
Behind this seemingly miraculous transformation considering, Even if you take good care of your body, you're still bombarded with toxins. They're in the food you eat, the water you drink and the air you breathe. Your body naturally produces toxins, too. It's up to your digestive system— especially the hard-working liver, the body's main detoxifier—to send those toxins packing.
In an ideal world, our bodies would eliminate toxins with ease. However, "as our exposure to toxins increases, our ability to deal with them decreases. You can boost your body's natural functioning by adopting good health habits every day, rather than going on a two-week program. Why not structure your own healthy lifestyle plan without having to pay for an expensive detox program.
Instead of detoxing, why not just de-junk? Give your body a break from digesting saturated fats and excess calories, especially from take-out meals, sugar, and processed foods. Plant-based foods, such as vegetables, fruits, nuts, herbs, whole grains, and legumes are less processed, so they are high in fiber, and have a high ratio of nutrients to calories.
This slows down their absorption rate, helping to satisfy your hunger and boost your well-being. Packaging, processing, and preserving foods reduces their nutritional value. Processed foods often include refined ingredients, such as salt, sugar, and chemically altered fats. Be wary when you're pouring something out of a box, opening a can, or reconstituting something with water. When the ingredient list includes a bunch of numbers and chemicals you can't pronounce, there are probably better food choices you could make.
The 10's of do-it-yourself detox
Help rid your body of contaminants acquired through daily exposure to pollution, cleaning products, processed foods and pesticides. By reducing stress, eating whole foods and approaching chores in a natural way, you can create a do-it-yourself detox to help you cleanse your mind and body. Enhance your body's ability to cleanse itself through a detox and instill healthier habits for life.
- Eliminate tobacco.
- Avoid foods containing artificial coloring or flavoring.
- Eat plenty of fresh fruit and vegetables.
- Take a multivitamin tablet daily.
- Drink at least eight glasses of water daily, and quit the soda thing.
- Avoid foods containing salt and high levels of preservatives.
- Eliminate your alcohol and reduce caffeine intake.
- Avoid full-cream dairy products, fatty meats, animal fats, and simple sugars.
- Focus on whole grains, and avoid processed grain foods like white pasta, white bread, white rice, and low-fiber breakfast cereals.
- Include some form of physical activity on a daily basis, or at last five times a week. Increase the intensity of exercise as your fitness improves.
One positive from do-it-yourself detox kits is that they provide a form of psychological comfort, because action is being taken and changes are being made to improve one's health. They offer a set plan to follow, which is often the structure people need to initiate change. Some people also don't like to think long term about their health, so a short-term plan sounds possible.
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This Heavy Metals Test is the world's first immediate method of screening heavy metals. We provide you with 2 tests to allow you to see the improvements you have been able to achieve through the lifestyle changes you have made. Now you can have a heavy metals test in the comfort of your own home.
Learn more about the Heavy Metals Test now!


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It's my birthday today... Declan Kelly made me this! Thanks to my Mam and my Dad and all the friendly ones sending me birthday greetings, best wishes, rice crispies, cake and kisses. And a very Happy non-Birthday to you all who's birthday it is not!
Wednesday, February 23, 2011
23rd February 1984
Hurpdeday Burpdadoo!
Posted by karate_feetus at 4:12 PM
Labels: Declan Kelly, herstory, photography
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St John's University – New York is classified as a doctoral-granting research university. This very large four-year institution has an estimated annual full-time enrollment of 10,000 students or more. More than half of degree-seeking students are enrolled full time, and 25-49% of undergrads reside on campus. This institution awards a balanced number of bachelor's degrees in both the arts and sciences and the professional fields. Graduate degrees are available in up to half of the fields available to undergraduate majors, and the school awards research doctorate degrees in a variety of fields. Most of these graduate degrees are awarded in professional fields other than engineering, such as education, health professions, social work, or public policy. According to data for fall enrollment, this institution has a low transfer rate, and at least 80% of undergraduates are enrolled full-time. Fewer than 20% of entering students are undergrad transfer students. Enrollment data shows that both graduates and undergraduates are enrolled at this institution, with only 10-24% of students enrolled in the graduate program.
SUNY College at Buffalo
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About SUNY College at Buffalo
School Information
General Information
Student Body SizeUndergraduate9,822
Student Age% Under 2572%
% 25 and Over27%
% Age Unknown0%
Attendance Type% Full-time80%
% Part-time20%
Race/Ethnicity% White66.5%
% Black12.2%
% Asian1.4%
% Latino4.9%
% Native American0.3%
% Race Unknown13.6%
% International student1%
Student ServicesCareer CounselingYes
Student Employment ServicesYes
Graduate Placement ServicesYes
Study AbroadYes
Day CareYes
Religious AffiliationNO
Campus settingCity
Intitution typePublic
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Academic Information
Art-Related Areas of StudyGraphic Design
Art Degrees OfferedBachelors Degree
Distance Learning AvailableYes
Calendar SystemSemester
Admissions Information
Open AdmissionNo
% Admitted42%
% Admitted Who Enrolled30%
Application RequirementsHigh School GPA
High School TranscriptRequired
High School RankingRecommended
College Prep CoursesRequired
Letter of RecommendationRecommended
Demonstration of CompetenciesRequired
Admissions ExaminationRequired
Test of English as a Foreign Language (International Students)Required
Standardized Tests% of Applicants Submitting SAT91%
% of Applicants Submitting ACT22%
Admitted Student SAT Scores 25th Percentile75th Percentile
SAT Critical Reading / 800530 / 800
SAT Math440 / 800540 / 800
Admitted Student ACT Scores 25th Percentile75th Percentile
ACT Composite18 / 3623 / 36
Tuition and Financial Aid
Tuition & FeesUndergraduate Application Fee$50
Graduate Application Fee$50
In-State Tuition & Fees$6,053
Out-of-State Tuition & Fees$13,953
Financial Aid% Undergraduates with Financial Aid87%
% Undergraduates with Loans83%
% Undergraduates with Grants78%
Average Undergraduate Financial Aid Award$ 0
Other CostsBooks and Supplies (Estimated)$990
Typical On-Campus Room and Board Cost$10,168
Off-Campus Room and Board Cost (Estimated)$0
On-Campus Living Expenses (Estimated)$2,100
Off-Campus Living Expenses (Estimated)$0
Living with Family, Living Expenses (Estimated)$0
Neighborhood Data for the Buffalo Area
Median Age33.5
Median Household Income$30,230
Total Population263,914
Age/Gender % Men % Women
Total47.2% 52.8%
Age 0-950.7%49.3%
Age 10-1751.7%48.3%
Age 18-2448.9%51.1%
Age 25-2946.4%53.6%
Age 30-3948.6%51.4%
Race/Ethnicity% White51.9%
% Black37.9%
% Asian3.0%
% Latino9.1%
% Native American0.6%
% Pacific Islander0.0%
% Indian (South Asia)0.9%
On Campus Housing ProvidedYes
Meal Plan ProvidedYes, number of meals per week can vary
Typical On-Campus Room and Board Cost$10,168
Off-Campus Room and Board Cost (Estimated)$0
Median Rent (Rental Units of Any Size)$480
Rental Price Distribution StudioOne BedroomTwo Bedroom
Under $50048.4%41.0%16.6%
$500 to $74938.8%42.2%0.0%
$750 to $9997.1%12.0%22.6%
Over $10005.7%4.8%7.4%
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