text:
Current and past research work by our ATLAS faculty, postdocs and other group members:
Develop new jet substructure and superstructure variables, including color-connection variables for new
physic searches (2010 to 2012 by Dr. Lowe and Prof. Gao): Talks at the ATLAS Standard Model, Higgs and Exotics Working Groups
from 2011 to 2012.
New physics searches with dijets in final state from ATLAS data (2010 to present by Dr. Bawa and Prof. Gao): Talks at the
ATLAS Jet and Exotics Working Groups since 2010. Published at New J. Physics 13 053044 (2011), and JHEP 01 (2013) 029
ATLAS Inner Detector, Muon Detector, and Trigger Upgrade R&D Projects (2009 to present by CSU Students and Prof. Gao): Talks at
ATLAS Inner Detector, Muon Detector, Trigger working group meetings since 2009.
Support ATLAS Event Data Model (EDM) in the trigger (2011 to 2012 by Dr. Lowe): Talks at the ATLAS Trigger
Core Software working group meetings since 2011.
Liaison for JetEtMiss and Physics Analysis Tools (PAT) working groups (2010 to present by Dr. Bawa): Talks at
the ATLAS Jet/Etmiss working group meetings since 2011.
Build up the first cloud-based Tier 3 computing cluster for US ATLAS (2011 to 2013 by Prof. Lin with CS
faculty and Ph.D students from Chinese research universities)
W/Z and egamma work (2009 to 2010 by Dr. Bawa, Prof. Gao and graduate student Brent Wilson): Talks at North
America e/gamma working group meetings.
Set up and run the CSU Fresno ATLAS Tier 3 cluster for ATLAS physics (2011 to present). The cluster was funded
by NSF MRI grant. Dr. Sergey Uzunyan from Northern Illinois Univesity (NIU) provided essential help in system design, setting up and maintaining
the cluster. The CSU Fresno ATLAS Tier 3 cluster has been running and heavily used for ATLAS physics by our group members. The cluster is currently
maintained by Jay Fowler and Choa-Lin Chou of CSU Fresno ITS department.
ATLAS performance studies, Particle identification (PID) fake rates using reflections of known physics processes
from incoming real ATLAS data and their impacts on ATLAS physics (2006 to 2008 by Prof. Gao): Talks at the ATLAS Standard Model, Higgs, Exotics,
Electron/Photon, Jet/EtMiss, and Tau Working Groups from 2006 to 2008.
Significance calculation and a new analysis method in searching for new physics at the LHC (2005 to 2006 by
Prof. Gao): Talks at the ATLAS Higgs Working Group in 2005 and 2007. Published in European Physical Journal C45, 659 (2006).
Electron/Pion Separation with LAr Barrel Module P15 using 2002 Testbeam Data (2002 to 2003 by Prof. Gao):
ATLAS Note: ATL-LARG-2003-013, published in Nucl. Instrum. Meth. A550, 96 (2005)


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Description: 1 videodisc (81 min.) : sound, color ; 4 3/4 in
Language: French
Special features: The cartoon according to Academy Award nominated writer/director Sylvain Chomet featurette; the making of "The Triplets of Belleville" featurette; three behind-the-scenes featurettes with scene commentaries; music video of the Academy Award nominated "Belleville Rendez-vous" song
Voices: Michèle Cacheteux, Jean-Claude Donda, Mari-Lou Gauthier, Charles Prévost Linton, Michel Robin, Monica Viegas
A bicyclist is kidnapped from the Tour de France by mysterious gangsters; his grandmother travels to the city of Belleville (which has a sardonic version of the Statue of Liberty in its harbor), where she tracks him down with the help of a musical trio gone to seed, the Belleville Triplets
MPAA rating: PG-13; for images involving sensuality, violence and crude humor
DVD, region 1, widescreen presentation; Dolby digital 5.1
Chiefly told without dialogue; French or Spanish with English subtitles; closed-captionedRelated Searches:
Grandmothers -- Drama.
Grandparent and child -- Drama.
Cyclists -- Drama.
Foreign films -- France.
Animated films.
Video recordings for the hearing impaired.
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Additional Credits:
Brunner, Didier.
Chomet, Sylvain, 1963-
Cacheteux, Michèle
Donda, Jean-Claude.
Gauthier, Mari-Lou.
Linton, Charles Prévost.
Robin, Michel.
Viegas, Monica.
Charest, Benoît.
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Grandmothers -- Drama.
Grandparent and child -- Drama.
Cyclists -- Drama.
Foreign films -- France.
Animated films.
Video recordings for the hearing impaired.
Added--04/4 advd
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Just outside the Marriott Hotel at 515 Queen Street is the sculpture known as Gifts. It shows what I believe to be a young aboriginal boy at play with birds overhead and at his feet. Sculpted by Rhyl Hinwood, it was commissioned in 1998 as a fountain work. Unfortunately, due to the ongoing drought and water restrictions, the fountain area has been re-potted with native plants. However, I feel the aesthetic of the sculpture has not been lost.
Rhyl Hinwood is quite a prolific sculptor, with international renown and a significant number of artworks around Brisbane. She also must have a keen sense of humour, as evidenced by the plaque associated with this sculpture. I thought it best that I transcribe this for reference here!
'I will bring you love', said the young lover,Oodgeroo of the tribe Noonucal
'A glad light to dance in your dark eye.
Pendants I will bring of the white bone,
And gay parrot feathers to deck your hair.'
But she only shook her head.
'I will put a child in your arms,' he said,
'Will be a great headman, great rain-maker,
I will make remembered songs about you
That all the tribes in all the wandering camps
Will sing for ever.'
But she was not impressed.
'I will bring you the still moonlight on the lagoon,
And steal for you the singing of all the birds;
I will bring down the stars of heaven to you,
And put the bright rainbow in your hand.'
'No,' she said, 'Bring me tree grubs.'
How appropriate that on Valentine's Day I can share this with you.
Cheers, I Love Brisbane, Wes.
Click Here for the Google Map Reference for this post.


text:
Burn is excited to announce the rollout of new equipment! For some time now, we have been in search of ways to make the Burn workout even more effective using new equipment.
>We have partnered with Stroops, the world's largest performance training company, to design a brand new, customized resistant band for the Burn workout.
Introducing the Slastix resistant bands for Burn! These new bands will allow Burners to do everything we have traditionally done with springs, and more! With the news slatix bands, we will be able to do more and get more from the Burn workout.
More movement!
For cardio specifically, we will be able to perform more dynamic and fun cardio segments, using the waist belt instead of just handles and the bar.
More exercises!
The new bands will have the capability of being used in different directions, allowing for burn to incorporate new exercises in class.
More modification options!
For Burners who need modifications due to injuries, the slastix will allow them to perform modifications safely, while still getting the resistance needed to be challenged during the workout. Clients will be able to hook their waist belts to the board in order to assist with proper push-up form, lunges, jogging, etc.
At Burn, we want our clients to get the most out of the 55- minute workout. We have spent quality time researching and comparing different equipment to find the perfect ratio of resistance and extension in each custom-made band. We are excited to implement the bands and maximize the Burn workout.


text:
Anne van Kesteren wrote: > > Quoting Brad Fults <firstname.lastname@example.com>: > >> I saw that right after I sent my last email. >> >> So now the question becomes: what does "evaluated" mean? Were the contents >> of the previous draft flawed in some way? > > The previous draft of the CSS 3 Text Module had several issues that have been > pointed out by fantasai on the www-style mailing list after which she became > an invited expert to work on the draft. > > At a guess I think she just did not get around checking those properties and > specifying them before the new draft was released. Or rather, I decided to release a new draft before completing everything since it's best, imho, to get feedback on existing parts sooner rather than later. Maybe I should have added a few exclamation points to the Status heading or something... > I certainly hope text-overflow:ellipsis will be added back which has already > one implementation and is quite useful. It will certainly be added back in, with some changes based on Dave Hyatt's feedback from the previous CR. ~fantasaiReceived on Thursday, 25 August 2005 03:02:29 UTC
This archive was generated by hypermail 2.3.1 : Monday, 2 May 2016 14:27:20 UTC


text:
Tuesday, March 23, 2010
Of Bunnies and Women
9 comments:
- elsiee said...
I'm with you, bunnies are perfection every day of the year!!
- March 23, 2010 at 11:44 PM
- Denise / Iktomi said...
Aww, these are so cute. Thanks a bunch for featuring my ceramic bunnies. It was sweet seeing them on your pretty blog!
Denise (Iktomi)
- March 24, 2010 at 5:25 AM
- boatbaby said...
Love the holiday neutral bunnies! The embroidered one and the last vintage one spoke to me. So sweet!
- March 24, 2010 at 6:07 AM
- vivian said...
sweet bunnies! I like then all.. hard for me to pick just one style. My home is very eclectic! a little bit of all!
have a great day!
- March 24, 2010 at 6:41 AM
- Hollie May said...
super cute! bunnies are so special dont you think?? little muffin bundles! thank you so much for adding mine in there too ;)
hugs times a million!
- March 24, 2010 at 8:54 AM
- Brayton Homestead Interiors said...
charming post. love the collection you gathered of bunnies. Thank you for including mine in your menagerie...oh nice!
- March 24, 2010 at 9:18 AM
- The Boob Nazi said...
I definitely thought the first picture was a pair of bunny crackers and imagined myself eating them... Oh well.
- March 24, 2010 at 9:50 AM
- noodle and lou said...
i have a big soft spot for the vintage ones...those bunny candles from the 40's are deeeelightful! what a darling collection you put together:) thank you so much for including my wee vintage guy! happy day to you! xox...jenn
- March 24, 2010 at 10:10 AM
- Lara Ann said...
found your bunnies from pinterest - and love them! cute blog!
- August 24, 2012 at 9:05 PM


text:
- Research article
- Open Access
Anti-inflammatory effects of Edaravone and Scutellarin in activated microglia in experimentally induced ischemia injury in rats and in BV-2 microglia
- Yun Yuan1, 2,
- Hao Zha1,
- Parakalan Rangarajan2,
- Eng-Ang Ling2Email author and
- Chunyun Wu1Email author
© Yuan et al.; licensee BioMed Central Ltd. 2014
- Received: 26 September 2014
- Accepted: 10 November 2014
- Published: 22 November 2014
In response to cerebral ischemia, activated microglia release excessive inflammatory mediators which contribute to neuronal damage. Therefore, inhibition of microglial over-activation could be a therapeutic strategy to alleviate various microglia-mediated neuroinflammation. This study was aimed to elucidate the anti-inflammatory effects of Scutellarin and Edaravone given either singly, or in combination in activated microglia in rats subjected to middle cerebral artery occlusion (MCAO), and in lipopolysaccharide (LPS)-induced BV-2 microglia. Expression of proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and inducible nitric oxide synthase (iNOS) was assessed by immunofluorescence staining and Western blot. Reactive oxygen species (ROS) and nitric oxide (NO) levels were determined by flow cytometry and fluorescence microscopy, respectively.
In vivo, both Edaravone and Scutellarin markedly reduced the infarct cerebral tissue area with the latter drug being more effective with the dosage used; furthermore, when used in combination the reduction was more substantial. Remarkably, a greater diminution in distribution of activated microglia was observed with the combined drug treatment which also attenuated the immunoexpression of TNF-α, IL-1β and iNOS to a greater extent as compared to the drugs given separately. In vitro, both drugs suppressed upregulated expression of inflammatory cytokines, iNOS, NO and ROS in LPS-induced BV-2 cells. Furthermore, Edaravone and Scutellarin in combination cumulatively diminished the expression levels of the inflammatory mediators being most pronounced for TNF-α as evidenced by Western blot.
The results suggest that Edaravone and Scutellarin effectively suppressed the inflammatory responses in activated microglia, with Scutellarin being more efficacious within the dosage range used. Moreover, when both drugs were used in combination, the infarct tissue area was reduced more extensively; also, microglia-mediated inflammatory mediators notably TNF-α expression was decreased cumulatively.
- Activated microglia
- Cerebral ischemia
- Edaravone
- Scutellarin
- Anti-inflammation
Ischemic stroke constitutes most of all strokes and is caused by obstruction of blood flow to the brain, which would initiate a complex cascade of metabolic alterations, including release of reactive oxygen species and inflammatory cytokines, activation of complement proteins etc. This would then exacerbate neuroinflammation and ultimately cause neuronal death. The innate immune response to induce postischemic inflammation is undoubtedly the hallmark feature for the progression of cerebral ischemia injury [1]. The key cell players in this are the activated microglia which can act as sensors to detect abnormal alterations in response to internal and external insults.
Microglial cells are the resident immune cells that mediate neuroinflammation in the central nervous system (CNS) [2] In neurodegenerative diseases and stroke, they are activated and engaged in different functions such as phagocytizing the toxic cellular debris, producing proinflammatory cytokines and enhancing neuronal survival by release of trophic factors [3]. In chronic activation, microglia are thought to contribute to neuronal damage via release of excessive proinflammatory cytokines and/or cytotoxic factors, such as nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1β), and reactive oxygen species (ROS) [4],[5]. As a corollary, inhibition or suppression of microglia to prevent over-reaction and inflammatory response of microglia may prove to be an efficacious therapeutic strategy to alleviate the progression of the neurological diseases.
In the search for potential drugs that may effectively suppress overt microglial activation, attention has recently been drawn to Edaravone and Scutellarin. Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one), a free radical scavenger that is currently used in the treatment of acute ischemic stroke as a neuroprotective reagent, has been shown to significantly reduce the infarct size, improve neurological scores, and decrease ROS generation [6]. More specifically, it can counteract toxicity from activated microglia [7]. Neuroinflammation in middle cerebral artery occlusion (MCAO) may be attenuated by Edaravone which acts through suppression of expression of proinflammatory cytokines in activated microglia [8].
The above studies have shown that both Edaravone and Scutellarin have anti-inflammatory effects in activated microglia. It remains to be determined which of the two common drugs is more potent in its anti-inflammatory effect in activated microglia, and whether there would be a cumulative therapeutic effect when both drugs were used in combination. This study was therefore aimed to investigate the anti-inflammatory effect of Edaravone and Scutellarin used either singly or in combination in experimentally induced cerebral ischemia, and in vitro in the BV-2 microglial cells. We sought to determine if a combination of Edaravone and Scutellarin at appropriate dosage may represent a more efficacious therapeutic strategy for treatment of neurodegenerative diseases in which activated microglia are implicated.
Changes in infarct size in MCAO rats given Edaravone and/or Scutellarin treatment
Microglia were activated after MCAO but were reduced in cell numbers following treatment with drugs
Edaravone and Scutellarin separately or in combination reduced the expression of inflammatory mediators in MCAO rats
Cell viability assay of BV-cells
We chose to perform our in vitro studies on BV-2 cell line instead of primary cultured microglial cells to ensure that we obtained enough cells for analyzing cell viability following drug treatment and also obtained adequate amounts of protein for Western blot analyses. The cytotoxicity data was obtained by the MTS assay for the effect of Edaravone and Scutellarin on BV-2 cells. A combined concentration of Edaravone (in the range of 50 μM to 200 μM) and Scutellarin (in the range of 0.27 mM to 1.1 mM) did not result in any significant cell death (Additional file 3: Figure S3). In this study we have used Edaravone at 100 μM with Scutellarin at 0.54 mM for all subsequent analysis.
Edaravone and Scutellarin separate treatment or in combination reduced the expression of inflammatory cytokines and iNOS in LPS-induced BV-microglia
Edaravone and Scutellarin separate treatment or in combination reduced the expression of ROS and NO in LPS-induced BV-cells
Neuroinflammation is a key contributor in the ischemic cascade after cerebral ischemia that leads to neuron damage and death. Activated microglia in inflammatory response have both beneficial and detrimental functions in the nervous system. During neuroinflammation, activated microglia remove cellular debris or invading pathogens, and release neurotrophic factors that regulate the microenvironment [16]. On the other hand, activated microglial cells are also elicited to produce a plethora of proinflammatory mediators, including TNF-α, IL-1β, ROS etc., which have been implicated in the pathogenesis of different neurodegenerative diseases, including Alzheimer's disease [17], Huntington's disease [18], Parkinson's disease [19], stroke [20] and hypoxic insults [21]. Therefore, a prevalent view is that activated microglia can aggravate the injury and subsequent neurodegeneration and they may serve as a prime therapeutic target in a wide variety of CNS diseases.
Here we examined the anti-inflammatory action of Edaravone and Scutellarin in activated microglia. Both Edaravone and Scutellarin reduced the infarct size in brain cortices of MCAO rats, inhibited production of pro-inflammatory mediators (TNF-α, IL-1β, NO and ROS) in MCAO rats and LPS-induced BV-2 microglial cells, as well as suppressed the experimentally induced increased expression of iNOS. Activated microglia appeared to emit many thin cytoplasmic processes when treated with Edaravone and Scutellarin. A possible explanation for this would be that both drugs had promoted the ramification of microglia whose ramified phenotype might represent a less active state. On the other hand, the possibility that Edaravone and Scutellarin can suppress the activated microglia by causing the cells to become more resistant to transformation into an amoeboid form, presumably a more active state, is considered. In any event, a significant diminution in distribution of activated microglia was evident in rats treated with both drugs combined. Furthermore, we have also provided the first morphological evidence that in comparison to Edaravone, Scutellarin was more efficacious in suppressing the expression levels of inflammatory mediators in activated microglia with the dosage used. Additionally, the two drugs in combination cumulatively depressed the expression of TNF-α both in vivo and in vitro. The results suggest that Scutellarin is more potent in therapeutic potential for various microglia mediated neuroinflammatory diseases.
Edaravone is a synthetic small-molecule free-radical scavenger. It has been reported to be effective in inhibiting the inflammatory responses [22], brain edema [23], ROS generation, and oxidative tissue damage [24]. Edaravone currently is being used clinically to treat stroke patients. Recently, Edaravone has been shown to possess neuroprotective and antioxidative effects on the brain after traumatic brain injury both in rat models and in patients [25]-[27]. The likely underlying mechanism for this is via inhibiting oxidative stress, leading to a decreased inflammatory response, and thereby reducing neuronal death and improving neurological function. Many in vivo experiments have similarly reported that Edaravone could mitigate microglial activation and suppress the production of proinflammtory meditors by activated microglia [8],[28]. Here, we confirmed the efficacy of Edaravone in inhibiting the expression levels of various inflammatory mediators in activated microglia as shown by Western blot analysis and immunofluorescence labeling.
It has been reported that Scutellarin could improve neuronal injury and had protective effect in rat cerebral ischemia at least related to its antioxidant property [29]. The neuroprotective effect of Scutellarin was associated with inhibition of the apoptosis-inducing factor pathway [9]. Recently, some studies have extended that Scutellarin could inhibit production of proinflammatory mediators induced by LPS in rat primary microglia or BV-2 microglial cells [14]. The present results are consistent with this. It is unequivocal from the present results that Scutellarin with the dosage used based on cell viability assay showed a greater potency in comparison to Edaravone in its anti-inflammation in activated microglia; hence, it is suggested that Scutellarin is endowed with a better therapeutic potential for various microglia mediated neuroinflammatory diseases.
While both Edaravone and Scutellarin have shown great efficacy in their anti-inflammatory effect in activated microglia, the potency of both drugs either administered separately or in combination has not been explored. We show here that when compared with Edaravone, Scutellarin was more effective in decreasing the infarct volume in MCAO rats. Very strikingly, Scutellarin alone suppressed the expression levels of inflammatory mediators to a greater extent when compared to Edaravone in activated microglia. Interestingly, the two drugs in combination cumulatively depressed the expression of inflammatory mediators notably TNF-α in activated microglia. Moreover, Edaravone when combined with a high dose of Scutellarin decreased the infarct volume extensively in MCAO rats. On the other hand, expression of other markers was depressed to a lesser extent in activated microglia between Scutellarin used separately or when combined with Edaravone. The possible explanation for this would be that expression of inflammatory mediators and changes in cerebral infarct volume are not synchronized and there are other mechanisms involved in response to brain injury. Notwithstanding, Scutellarin and Edaravone when used in combination can produce a cumulative protective effect in ischemia brain injury.
In the present results, the infarct volume of the cerebral cortex in MCAO rats was obviously decreased when treated with Edaravone and Scutellarin separately or in combination. Associated with this was the drastic diminution in numbers of activated microglia. Recent studies suggest that Toll-like receptors (TLRs), especially TLR2, may have a key role in the progression of brain damage induced by cerebral ischemia [30]-[33]. It has been documented that a marked long-term induction of TLR2 expression in microglia activation after transient MCAO, suggesting an important role of TLR2 activation after stroke [31],[33]. A significant decrease of the infarct volume in TLR2 deficient mice compared to wild type mice and altered microglia activation profiles has been observed [33],[34]. The present results showed that the infarct size was considerably reduced in MCAO rat brain treated with drugs in comparison to untreated MCAO rats. Meanwhile, the activated microglia were not only reduced in numbers but many of them had also appeared ramified phenotype in the ipsilateral cerebral cortex of MCAO rat brain notably in rats given the combined drugs. The possibility is that both Edaravone and Scutellarin can cumulatively suppress the upregulation of TLR2.
We show here that Edaravone and Scutellarin when applied in combination cumulatively decreased the expression of inflammatory mediators, being most pronounced for TNF-α in activated microglia. TNF-α contains soluble and membrane-bind TNF-α [35]. Activated microglial cells are the major producers of soluble TNF-α within the first 6 hours after cerebral ischemia [36]-[38]. TNF-α converting enzyme (TACE), also known as a disintegrin and metalloprotease 17 (ADAM17) [39], is involved in multiple cell signaling pathways, including p44 mitogen-activated protein kinase (MAPK)-dependent manner [40]. In light of the above, it needs to be further explored whether pretreatment with Edaravone and Scutellarin would directly suppress the expression and function of ADAM17, or act on p38/p44 MAPK to synergisticly decrease TNF-α expression. Scutellarin and Edaravone might target on different sites of TNF-α expression and this should be considered. Studies have shown that inhibition of TNF-α using its antibody reduces infarct volume after MCAO [41],[42]. This may offer an explanation on why Edaravone and Scutellarin in combination can markedly reduce the infarct volume after MCAO. Apart from TNF-α, it is interesting to note that there was lesser reduction in the expression of other inflammatory mediators following Edaravone, Scutellarin or combined treatment. One possible explanation for this may be that the expression of these inflammatory mediators has reached the basal levels with either Edaravone or Scutellarin treatment. Therefore, further treatment with two drugs in combination could not suppress the expression further except for TNF-α, which may be more sensitive to the drugs.
A similar phenomenon and possible underlying mechanism are also found on NO expression in BV-2 cells treated. The NO expression was markedly reduced in BV-2 cells treated with Scutellarin or Scutellarin + Edaravone as compared with Edaravone alone within the dosage range as determined by cell viability assay. This is consistent with iNOS expression as manifested by inmunofluorescence labeling and Western blot.
The present morphological evidence and protein analysis indicate that Scutellarin is more potent in suppressing neuroinflammation induced by activated microglia with the dosage used, but the underlying molecular mechanism remains uncertain. Studies have shown that Scutellarin is capable of attenuating the expression of not only those proinflammatory molecules whose expression depends on the activation of NF-αB (a major mediator of microglial inflammatory response), but also those via transcription factor signal transducer and activator of transcription 1α (STAT1α) transcription factor [14]. Recent studies by us have shown that the Notch signaling pathway is involved in microglial activation and microglia-mediated cytokine production by promoting the expression of NF-κB [43],[44]. Notch-1 signaling was also identified to regulate microglial activation via NF-αB pathway after hypoxic exposure [45]. Indeed, there is evidence that Notch/NF-αB signaling pathways are involved in inhibition of microglial activation by Scutellarin and production of inflammatory mediators (unpublished data).
We show here that both Edaravone and Scutellarin could decrease infarct volume, frequency and distribution of activated microglia as well as suppress the production of inflammatory mediators in activated microglia in MCAO rats. Scutellarin appeared to be more potent in its anti-inflammatory effects when compared with Edaravone with the dosage used. Remarkably, the two drugs in combination cumulatively decreased the infarct size in the cerebrum and diminished the ischemia-induced inflammatory mediators being most pronounced for TNF-α expression. Thus, Scutellarin or along with Edaravone may prove to a more efficacious therapeutic strategy for treatment of microglia mediated neurodegenerative diseases, such as stroke.
Ethics statement
This research work has been carried out within an appropriate ethical framework. While handling and use of rats, ethical guidelines as stated in the National Institutes of Health Guide for the Care and Use of Laboratory Animals were adopted. All experimental protocols and use of animals were approved by Kunming Medical University and all efforts were made to minimize the number of rats used and their suffering.
Animals and experimental groups
Surgical procedures and number of rats used in various treatments
Sham-operated + saline group (sham)
MCAO + saline group (MCAO)
MCAO + Edaravone group (E)
MCAO + Scutellarin low dose group (SL)
MCAO + Scutellarin high dose group (SH)
MCAO + Edaravone + Scutellarin low dose group (E + SL)
MCAO + Edaravone + Scutellarin high dose group (E + SH)
n = 5
n = 5
n = 5
n = 5
n = 5
n = 5
n = 5
Double inmunofluorescence
n = 15
n = 15
n = 15
n = 15
n = 15
n = 15
n = 15
Western blot
n = 15
n = 15
n = 15
n = 15
n = 15
n = 15
n = 15
n = 35
n = 35
n = 35
n = 35
n = 35
n = 35
n = 35
Anesthesia of the rats was achieved by an intraperitoneal injection of sodium pentobarbital (50 mg/kg). The surgical procedure followed that described previously by us [46]. Briefly, a circular aperture 3 mm in diameter was burred in the right parietal bone with a dental drill, and the main trunk of the middle cerebral artery (MCA) was exposed and cauterized. In the sham-operated rats, the same surgical procedure was followed but the MCA was not cauterized.
Injection of Edaravone and Scutellarin
The rats in the respective groups were given an intraperitoneal injection of Edaravone (5 mg/kg dissolved in saline; Cat. No. H20070051, Jilin, China) and/or Scutellarin (50 mg/kg or 100 mg/kg dissolved in saline; Cat. No.131021, Shanghai Winherb Medical Technology, Shanghai, China) at 2 h before and at 12, 24, 36, 48, 60 h after MCAO; rats were sacrificed at 1, 3 and 7 d after MCAO.
TTC assessment of infarct size
Thirty five rats were used for assessing infarct size (n = 5 for each group). The rats were killed at 3 d after MCAO. The brains were rapidly removed, frozen at -20°C for 30 min. A total of six 2 mm thick coronal sections of the brain were then cut in a rat brain matrix starting at the frontal pole. This series of brain sections totaling 12 mm in thickness included the entire infarct area caused by the MCAO. The sections were incubated for 30 min and stained with 1% triphenyltetrazolium chloride (TTC) at 37°C protected from light. After this, sections were fixed in 2% buffered formaldehyde solution for 4 h. The cerebral infarct area as outlined in white in MCAO rats as well as in rats after various drug treatments, is depicted in Figure 2. Infarct areas in each section were measured using Image J software. A correction for edema was made according to the following formula: infarct area × (area contralateral hemisphere/area ipsilateral hemisphere) [47],[48]. Cerebral infarct volume was measured as a percentage of the total contralateral hemisphere, as calculated with the following formula: total infarct volume = sum of infarct volume of all sections measured (corrected infarct area × 2 mm for each section)/total contralateral hemispheric volume × 100.
Cell viability assay of BV-cells
Cell viability was assessed by CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega, Fitchburg, WI, USA; Cat. No. G3580). To determine the cytotoxic effect of Edaravone and Scutellarin on BV-2 cells, cells were plated into 96-well microplates (104 cells/well) and cultured for 24 h. They were subjected to a combined treatment of Edaravone (in the range of 50 μM to 200 μM) and Scutellarin (in the range of 0.27 mM to 1.1 mM) in each well containing 100 μl of culture medium for 1 h in triplicates. Briefly, 20 μl of MTS(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) reagent was added to each well (final concentration, 0.5 mg ml-1) and the plate was incubated for an additional 4 h. The optical density (OD) was then read at 490 nm using a microplate reader (GENIOS, Tecan, Switzerland). The assays were performed in triplicate.
BV-cell culture and treatment
BV-2 murine cells were cultured in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal calf serum (FCS) at 37°C in a humidified incubator under 5% CO2. The cells were divided into control, LPS-induced, LPS + Edaravone, LPS + Scutellarin, and LPS + Edaravone + Scutellarin groups. The cells was pretreated with Edaravone (100 μM), Scutellarin (0.54 mM), and Edaravone + Scutellarin 1 h at 37°C in a humidified incubator under 5% CO2. The dosage of Edaravone and Scutellarin used was based on cell viability assay when the drugs were used separately (data not provided). After incubation, the medium was discarded and the cells were washed with PBS, and then incubated with LPS (1 μg/ml, Sigma-Aldrich, MO, USA) for 3 h. The culture medium was replaced with basic DMEM before treatment. For controls, the medium was replaced with basic DMEM incubated in a chamber 95% air 5% CO2. Finally, proteins were extracted for Western blot analysis.
Double immunofluorescence labeling in the cerebrum and BV-cells
Antibodies used for Western blotting and staining
Catalog number /RRID
Mouse monoclonal
BD Pharmingen San Jose, CA USA
Rabbit polyclonal
Chemicon, Temecula, CA, USA
Rabbit polyclonal
Chemicon, Temecula, CA, USA
Mouse monoclonal
Sigma-Aldrich, MO, USA
BV-2 cells were fixed with 4% paraformaldehyde in 0.1 M PBS for 20 min. Following rinsing with PBS, the coverslips with adherent cells were used for immunofluorescence staining. In each group, BV-2 cells were incubated with the primary antibodies as described above overnight 4°C. Subsequently, the cells were incubated in FITC/Cy3-conjugated secondary antibodies for 1 h at room temperature. After washing, the coverslips were mounted using a fluorescent mounting medium with DAPI. All images were captured using a confocal microscope. The isotopic control confirmed the specificity of all primary antibodies used (data not shown).
Western blotting analysis for MCAO tissues and BV-cells
A total of 105 rats were used for Western blotting analysis. The sham-operated and MCAO rats given saline, Edaravone and/or Scutellarin injections were sacrificed at 1 day (n = 5), 3 days (n = 5) and 7 days (n = 5), respectively. The control or ischemic cortex derived from each group was frozen in liquid nitrogen and stored at -80°C. Tissue samples from various groups were homogenized with protein extraction reagent (Pierce, IL, USA) containing protease inhibitors. For BV-2 cells of each group, the cells were lysed with lysis buffer, mechanically scraped off with a rubber scraper and centrifuged at 13,000 rpm for 25 min. Protein concentrations of both tissues and BV-2 cells were determined by using a protein assay kit (Bio-Rad, Hercules, CA, USA; Cat. No. 500-0002). Samples of supernatants containing 50 μg protein of tissues or 40 μg protein of BV-2 cells were loaded and heated to 95°C for 5 min, and were separated by sodium dodecyl sulfate-poly-acrylamide gel electrophoresis in 10% or 12% gels, in a Mini-Protein II apparatus (Bio-Rad, CA, USA). Protein bands were electroblotted onto polyvinylindene difluoride (PVDF) membrane and blocked with non-fat dried milk for 1 h. The membranes were incubated with iNOS (mouse monoclonal IgG 1:1000) (BD Pharmingen, San Jose, CA, USA; Cat. No. 610432), TNF-α (rabbit polyclonal IgG 1:1000) (Chemicon, International, Temecula, CA, USA; Cat. No. AB1837P), IL-1β (rabbit polyclonal IgG 1:1000) (Chemicon International; Cat. No. AB1832P), and β-actin (mouse monoclonal IgG 1:10000) (Sigma; Cat. No. A5441) primary antibodies diluted in Tris-Buffered Saline-0.1% Tween (TBST) overnight at 4°C. They were then incubated with the secondary antibodies, either with horseradish peroxidase (HRP) conjugated anti-rabbit IgG (dilution 1:3000) (Thermo Scientific; Cat. No. 31460) or anti-mouse IgG (dilution 1:20000) (Thermo Scientific; Cat. No.31430). Protein was detected by chemiluminescence kit (GE Healthcare UK Limited, Bucks, UK) following the manufacturer's instructions and developed on the film. The band intensity was quantified in Image J software (National Institutes of Health, NIH, USA). All experiments were repeated at least in triplicate.
Measurement of reactive oxygen species by flow cytometry
Intracelluar ROS production in BV-2 cells of different groups was evaluated by detecting the fluorescence intensity of 20, 70-dichlorofluorescene, the oxidized product of the fluoroprobe 5-(and 6)-chloromethyl-20, 70-dichlorodihydrofluorescein diacetate (CM-H2DCFDA, Molecular Probes, Invitrogen; Cat. No. C6827) following the manufacturer's instruction. The amount of ROS production was considered to be directly proportional to fluorescence intensity given as cell counts and fluorescence intensity at the y-axis in the flow cytometry.
Real time measurement of free nitric oxide
BV-2 cells were treated as described above and the cells were seeded directly onto glass chamber. NO production was measured by NO detection Kit for fluorescence microscopy (Enzo Life Science, NY, USA; Cat. No. ENZ-51013-200) according to the manufacturer's instruction.
Statistical analyses
The data are presented as mean ± standard deviation (±SD). Statistical significance was evaluated by one-way analysis of variance (ANOVA) followed by post-hoc test. The difference was considered statistically significant when p <0.05. SPSS 16.0 statistical software was used to analyze data.
Authors' contributions
CYW and E-AL conceptualized and designed this study. YY, HZ and RP conducted the experiments. E-AL, YY and RP analyzed and interpreted the findings. YY led to write the manuscript and was responsible for submitting the final manuscript. All authors read and approved the final manuscript.
Additional files
This project is supported by National Natural Science Foundation of China [Project number 31260254, CY Wu], Applied Basic Research Program of Yunnan Province [Project number 2011FB243, CY Wu] and National University of Singapore [Project number R-180-000-140-592 E-A Ling].
Authors' Affiliations
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


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Our Access Project aims to work with local disabled people and the Access Group to find the most accessible places in the borough.
Our Access K and C "Most Accessible"report shows the most accessible locations in the Royal Borough as decided upon by members of our Access Group.
If you would like to be involved, or if you know of local accessible venues, feel free to get in contact with Mariya on Friday afternoons, on 020 8960 8888 or by email to email@example.com
Mariya introduces herself:
Hello friends, my name is Mariya Stoeva, I am an active member of ADKC, participating in many groups and activities, I've also been a trustee for a few years. I have just become the coordinator of a new ADKC project called ACCESS KENSINGTON AND CHELSEA, which sets out to find the most accessible places in the Royal Borough, making it easier for disabled people like me to find such places. Together let's make Kensington and Chelsea the most accessible place in the whole country!


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In addition to the special and technical symposia, workshops and other program, the following additional programming will be provided for undergraduate students.
Special funding for the undergraduate program is provided by the American Chemical Society
Thursday, October 22nd
Join us for the "Choosing Your Path" workshop from 8-12. Junior College Room
Come meet and talk with chemistry professionals in the Career Experience Panel Discussion from 4:30 - 6:00 pm. Blum 222
Friday, October 23rd (Mole Day)
Undergraduate Research Poster Session from 9:30 - 11:30. Fulkerson Center
Join us for a Mole Day Lunch from 12-1. There will be a special appearance by the ACS Mole! Graduate recruiters from will be on hand to meet with students interested in graduate programs at our sponsor institutions. Fulkerson Center
We'll wrap the celebration up with a Chemistry Demonstration show beginning at 5pm. Agenstein Hall Room 324
Saturday, October 24th
Students interested in chemical education are encouraged to attend the High School Teacher Workshop from 9 - 12. Agenstein Hall Room 330


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Although known for its genuine southern hospitality, you may be surprised to find that Atlanta is far more than columned mansions, southern belles and lemonade. The capital of Georgia is a very cosmopolitan city known to be a great business center as well as a fantastic vacation spot.
Its rich in history and home to the Martin Luther King Jr. Center. There are many museums, art exhibits and theatre productions for you to enjoy. Enjoy the jazz while you feast on a meal sure to satisfy your every craving. Whether youre in town for business or pleasure, Atlanta will impress you enough to come back again and again.


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We improved the wellbeing of people with a long-term condition through an intervention that offers stepped care approaches to psychosocial interventions in primary care.
The care for people with long term conditions is predominantly delivered by medical and other clinical staff and whose focus is primarily on the physical consequences of the "illness". Our proposal was informed by our work with people with addictions. Addiction has a relapsing remitting course and is frequently referred to as a long-term condition. The combination of psychosocial interventions (delivered by a multi-disciplinary team of trained practitioners and people with a lived experience) with evidence based clinical care (delivered by medical and other clinical staff) is considered "normal" best practice in this particular long-term condition. We were struck that this is not the case with other long-term conditions where care is predominantly delivered by medical and other clinical staff and whose focus is primarily on the physical consequences of the "illness".
People enter our project as part of their long-term condition review or following a new diagnosis in primary care. The intervention is based on their 'Patient Activation Measure' score (PAM; an indicator of their ability to self-care). Patients are offered stepped care approaches to psychosocial interventions, ensuring those with the lowest PAM scores are offered the most intensive intervention.
What are our results?
PAM is used to identify patient needs for intervention and to measure success, with an increase in PAM linked to a decrease in healthcare costs. For our latest data, please see our most recent newsletter.
"I was completely blown away with the support you were offering me and my wife after meeting you in clinic, really pleased with you both and this service is a wonderful idea." A Person.
"It's amazing how much you want to help me and how kind and supportive you both are when you talk. I was really looking forward to our chat today." A Person.
"It's a great feeling knowing that someone cares about us and will offer us great advice." A Person.
"We've noticed a massive difference since the team started working at our surgery." A GP
"[Person] is a completely changed person from the one I sent through. They've made some big changes and the improvement to their confidence is visible." A Practice Nurse.
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Some of the best colleges are like a classic Ferrari roadster. A college education at an Ivy League school, much like having a Ferrari, will never go out of style, it has a great reputation, and although it might be expensive, it seems worth every penny. But is it the best college for you? What are the most important factors for you to consider when selecting your future school?
The fact that driving a sports car makes you immediately appear to belong in the ranks of the rich and famous makes it seem worth it. This is the same motivation some people have for choosing which college to attend. But, there are a lot of other reasons for going to college. Besides improving your reputation, underlying benefits exist.
Whatever your reasons are for continuing your education, or how you hope to benefit from your achievement, you are presented with a plethora of choices. How do you know which is the best college for you?
How Important is a College's Reputation?
According to Forbes Best Colleges list of 600 undergraduate institutions, the best colleges are based on educational outcome, not reputation. Schools like Brown, Columbia, Cornell, Dartmouth, Harvard, U Penn, Princeton and Yale have stringent admissions criteria and draw some of the smartest minds, and some schools are also famous for their sports programs, such as the University of Texas (Longhorns), Ohio State (Buckeyes), or UCLA (BRUINS). However, these are based upon reputation, not the quality of the education.
What Factors Contribute to Educational Outcomes?
The educational outcomes at some of the best colleges in the United States are based on a school's faculty (the quality of the teachers is very high on the list), career prospects for its students, graduation rates, and the expense of tuition. Today, many of the best colleges offer special programs for the military and students who fall into the middle class category (such as MCAP at UCLA) which can make attending more affordable.
What Additional Factors Should You Consider?
Of course, every student's situation is different. A perfect fit for you may not resonate the same with your classmate. Think about the location of the school and how far you want to be away from home. Consider extracurricular activities available and how you plan to spend your time outside of the classroom. Speaking of classrooms, would you prefer classes of about 30 or giant lecture halls? The size of the student body at the school you select will play a major factor in the amount of in-class attention you receive.
Weigh each of your answers to these questions and it will surely steer you in the right direction, ultimately leading to the best college for you.
Westface College Planning can help navigate the financial aid process from start to finish. To learn how we can help, call us at 650-587-1559 or sign up for one of our Tackling the Runaway Costs of College Workshops or Webinars.
Photo Credit: Alan Cleaver


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It's the weekend, y'all!
A quick but heartfelt thanks for all your support, sympathy and encouragement after my last post. Honestly, just writing about it helped me hash out my feelings a bit, which was helpful since I had been bottling it all up.
No decisions for now, and I've got plenty on my plate to keep me busy, which is good. I will say that my kids' behavior over the last couple of days has me thinking: WHY did I want another baby again? Man oh man, they are quite the handful these days.
What are you up to this weekend? We've got Maya's last soccer game of the season followed by a team party. Then we are dropping the kids off with my parents for the night and will try hard not to burn rubber as we speed away. Date Night, here we come!
Chat away 😀


text: A nasty ghost, a photography-savvy teen and her stressed, uncommunicative family form the backbone of this all-too-predictable, though at times engaging, mystery. Pink-haired high-school misfit Alexis has built up a shield of disdain to the point that she is essentially friendless. Her parents are so self-absorbed they haven't noticed that their younger daughter, Kasey, is exhibiting increasingly bizarre behavior, including an obsession with dolls that has alienated her peers. Worried that her sibling is going mad, Alexis is moved to accept the help of two unlikely candidates—an unflappable cutie who continues his pursuit of her despite her initial rebuff and a cheerleader who has recognized that Kasey's oddness is not mental illness, but a case of supernatural possession. Strong characterization will draw readers in. Despite their realistic shortcomings, both primary and secondary characters are unique and satisfyingly complex. The plotting, however, is less effective. A selection of horror tropes—from spooky dolls to small-town secrets—fails to come to life, and the final healing of rifts in the girls' family seems contrived. (Supernatural thriller. 13 & up)


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Over the years I have gone to many kindergarten workshops and classes. I have read more magazines, books and now blogs than I can remember. So I apologize that I do not remember where I found this wonderful idea but it is another one of those "old" ideas that I LOVE!
I have been creating this book for maybe ten years now. This year I made it part of my January math stations. At station number one, the children had to draw one mommy penguin and three baby chicks. I had several sheets in the bucket to give the children an idea of how to draw penguins.
Then when everyone finished all the stations. I collected all the penguins and cut them out and put them together into a class book called, Five Little Penguins.
I read the book, Antarctica to the children. We discussed sunsets and then I let the children get to work on water coloring sunsets.
If you would like a copy of Five Little Penguins Click Here :)


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Zishy rocks. It brings us awesome models such as Noelle Easton. This girl has the best natural curves. You're going to love her big tits! Zishy is a site about photography, fun, tease, and of course, attractive young women. The aim is to excite and entertain through an innocent and playful means. For all those bored (and possibly overwhelmed) by porn and nudity on the Web, but still interested in seeing beautiful girls in photos, Zishy is for you, AND it updates 3-4 times a week. Nice!


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Indix, the Google of products
Getting competitive intelligence on products and keeping up to date used to be hard but Indix has the answer
Competitive intelligence can be hard to come by particularly in complex markets crowded with products from many manufacturers. Consider a pharmacy or supermarket chain, businesses in which pricing of many product lines is ultra competitive. If they don't know for a week or more that a competitor has reduced the price of some common consumer product they stand to lose more than the revenues from being undersold on that one item; they risk the total loss of consumers who go to the competition and buy that and other products while they're there so a small pricing edge can translate into a significant revenue hit.
To solve this kind of problem Indix is building what they plan to be the "world's largest product database"; as the company explained to me, they want to be the Google of products and they're off to a good start with something like 400 million products currently indexed. They plan to reach more than 1 billion in the near future.
The data Indix gathers and stores includes "catalog" data, which are static product details, and "offers", dynamic data that can change frequently.
To gather this data Indix uses crawlers along with intelligent algorithmic filtering to mine Web sites for product data on a roughly weekly schedule. Machine learning keeps the amount of human interaction to a minimum and, at present, only data in English is ingested (support for other languages is on the way). Of course, there's an enormous range of formats that product data can come in so a team of five humans backends the mining to ensure relevance and quality.
So, what can be done with this vast ocean of detail? Indix provides some very powerful analysis and tracking tools; for example, consider a fictitious shore retailer, Shoes99. They carry a number of lines and styles and want to ensure that their pricing and product mix is competitive.
To do this Shoes99 would select all products they carry on the Indix site and then Indix would scour their market data and produce a dashboard showing the products priced lower than other retailers, those priced higher, those that competitors charge more and less for, which products only Shoes99 carries and which only their competitors carry, and so on. For buyers and sales managers this makes product planning and pricing much easier and far more timely.
Product data can also be displayed over time and aggregated data, such as product mix by category, can be analyzed as well as with key indicators which makes upwards reporting far easier.
As an Indix user you can create alerts for product price drops, competitors running out of stock, new products, and so on, as well as generate reports, search for products, and import and export data. There's also an API that allows for integration with other systems such as accounting, in-house analytics and reporting tools, as well as other applications and services.
Indix currently has 15 large customers and they claim that they're enabling a 15% to 20% productivity improvement and a 2% profit improvement which, in businesses with low margins such as grocery and pharmacy, can be a significant gain.
Indix's system (priced on application) is amazing and and beautifully designed and has value to both retailers and brands - in particular, mass market brands which have a huge incentive to keep Indix updated to ensure that buyers can find their products as soon as they come to market. Indix is definitely a company to watch.


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Get smarter! Minecraft: Education Edition on its way!
Earlier this year, we announced Minecraft: Education Edition, a new version of Minecraft designed to help kids learn real good and make better brain-words about stuff. We're pleased to say a Beta program will launch this May, involving over 100 schools from 30 countries all around the world. Then, in June, we'll launch an early access program for teachers to download and try for free. Those lucky things!
Since 2011, we've been working with profs, boffins and other cleverclogs to bring classroom subjects to life using Minecraft - and not just essential topics like ocelot domestication and Nether portal design, but useful things like geometry, coding, architecture, ancient history and electrical engineering. The new Education Edition hopes to make that even smoother, using feedback from the Beta and early access period to fine-tune the controls teachers need to create an even richer learning environment.
When it launches, Minecraft: Education Edition early access will be available in 11 languages and 41 countries, and released on the hot new operating systems, Win 10 and OS X El Capitan. Gold stars all round!
Find out more on the Education Edition blog.
Marsh - @marshdavies


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I learned about cookie butter spreads via instagram photos, specifically from IGers in the Philippines. It has become sought-after that it's being sold at expensive prices. One of the famous brands being sold in Manila is the Trader Joe's Cookie Butter and though I came across it in Rockwell, I didn't buy it because I found the cost unreasonably expensive.
I mentioned the cookie butter spread to a friend who was about to go to America for a holiday. However, when she got there, all three Trader Joe's branches she went to were sold out on the cookie butter. According to one of the owners, a lot of Filipinos hoard the cookie butters. So instead of the TJ Cookie Butter, my friend brought home the original cookie butter spread made from Belgium, Lotus Biscoff Spread (a.k.a. cookie butter).
On the other hand, I discovered that we can also get Lotus Cookie Butter spreads here in Australia via the online store: DutchStore.com.au (thanks to an IGmate for the tip!) So I went online and got ourselves a bottle of each: Lotus Speculoos, Lotus Speculoos with Chocolate and Duo Penotti.
We have tried all three and all were good-tasting spreads. The Speculoos spread tastes like the real cookie butter wherein I can very much taste the cinnamon and nutmeg. I like the Speculoos spread enough to have it once in a while and alternate it with other spreads. I've learned a tip to put it in pancakes and oatmeals so maybe one day I'll try that too.
The Speculoos with Chocolate tastes exactly like a cookie butter dipped in chocolate. I ordered this in the hopes that the Husband will like it because he's a choco-holic, however, he wasn't impressed with this spread. He said it wasn't chocolate-y enough. It seemed okay with me though, me being an average chocolate lover.
Because of this Speculoos phenomenon in the Phils, I realized we have a very boring selection of spreads at home. For years, it has always been just peanut butter, strawberry jam and Nutella for us.
I'm glad we got to try these Speculoos spreads. We weren't blown away by it but we appreciate being able to try other spreads for a change. This also got me thinking to have a look out for different spreads available in cafes here. You'll know know, right? I might just find a delicious spread that our home will love.


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On Saturday, Nov 17, the Iles House played host to two groups, the docents who provide guide and host services throughout the year, and the local chapter of the Daughters of the American Revolution.
Here the docents discuss their duties and schedules over lunch. David Stevens (ivory sweater) provides advice and feedback.
Dick describes the Iles House as "Springfield's home," as it has rapidly become a gathering and meeting place for many historic-minded associations.
The DAR, descendants of ancestors who achieved American independence, has existed since the late 1800s. 2006 marks the 60th anniversary of the Caleb Hopkins chapter of central Illinois.
That eerie face in the silver tray is David Stevens. Pictures are by Dulany Sriner.
Friday, November 24, 2006
Thursday, November 23, 2006
Happy Thanksgiving
Posted by Will Howarth at Thursday, November 23, 2006 No comments:
Thursday, November 16, 2006
AIG Reception
On Nov 15, employees of AIG (American International Group) held a reception at Iles House. Over 50 attended the event, after a day of meetings at the Springfield office (former Franklin Life). Catering was by Augie's Front Burner of Springfield.
Several executives of AIG were present, along with managers from the Midwest region. On behalf of Iles House, Dick Hart welcomed the group and thanked AIG for the gift of a parking lot at Seventh and Cook.
Several executives of AIG were present, along with managers from the Midwest region. On behalf of Iles House, Dick Hart welcomed the group and thanked AIG for the gift of a parking lot at Seventh and Cook.
Posted by Will Howarth at Thursday, November 16, 2006 No comments:
Thursday, November 09, 2006
Vachel Lindsay
November 10 is the birthday of Vachel Lindsay. He was born in Springfield in 1879 and died there in 1931. He studied art in Chicago and New York and then traveled all over the USA, lecturing and reading his poems. He remains today Springfield's most famous writer.
An article in today's Journal-Register describes the recent discovery of a letter that Lindsay wrote in 1928 to Susan Wilcox, his English teacher at SHS. The letter passed to Elizabeth Graham and then to Jaqueline Jackson, and only by good luck recently came to light.
The letter praises Miss Wilcox for her work on a student literary magazine and admires the writers' names, which ranged ". . . from Portuguese to Lithuanian. I read back into the family histories of some of them, just from the names. The old Springfield names have a special thrill for me, even though none of them represent intimates."
Lindsay's letter has returned to the library of SHS. The picture above is of his wife, Elizabeth, and their children, all described in the Wilcox letter. An extensive collection of Lindsay manuscripts survives at Firestone Library, Princeton University.
Posted by Will Howarth at Thursday, November 09, 2006 2 comments:
Thursday, November 02, 2006
Candlelight Tour
On October 27-28, Iles House hosted its Candlelight Tour, a repeat of the popular seasonal event held last year. With its rooms lighted with candles, the House takes on a glow that reminds visitors of the past.
About 250 visitors attended, many of them from outlying parts of Sangamon county. Here they view pictures that depict moving the House to its present site in 1998.
Iles House Foundation president Dave Barringer (white shirt) gives visitors an overview of the House purpose, to serve as a museum of Springfield history and to raise awareness of the need for historical preservation in central Illinois.
A group of volunteer guides help to interpret the House, and several of them wear period costumes from the 1840s, the ladies in wide hoop skirts and the gentlement in frock coats and top hats. Amid the glow of candles, long ago seems to come alive.
Pictures by Dulany Sriner
Posted by Will Howarth at Thursday, November 02, 2006 5 comments:
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BORLA XR-1 Raceline products are the choice of todays hottest drag and oval track racers. Featuring a patented uninerrupted-flow design and multicore technology. BORLAs XR-1 mufflers actually outflow a straight piece of pipe in repeated testing. XR-1 race products are light weight and constructed of high quality austenitic stainless steel for track-tough durability. They meet the strictest noice requirements and use sound absorbing stainless steel pcking to protect against burnout. Hands down, BORLAs XR-1 Raceline offers the best balance of sound and power.
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New Ink! 15-04
by Schnauzevoll
Hey there, Internet!
While changing my haircolor I also changed my hairstyle a bit which caused me to expand the sidecut a bit, too – again. And again I was left with an empty space around the tattoo on the side of my head. This happened quite a few times since I originally got that tattoo:
That one, after years of drooling, was my very first tattoo that I got when I finished 10th grade and a present from my mom for doing good during the final exams. I wanted a bat, in my neck. The artist didn't want to do the bat the way I wanted it to be (with full wings,…) and didn't want to place it the way I wanted (exactly at the beginning of my hair) but I was stupid, naive and too excited to say no. So I got the bat without full wings, lower than I wanted but I got it.
Sorry for very bad quality, thats how digital photos from 2004/2005 looked like… Anyways. At first I was happy, thought the wings looked a bit skeleton like and well, it was a bat, it was my bat tattoo. After a while I learned more about what makes a good tattoo, what to look for when deciding for an artist/shop. Should have decided for a better artist and should have brought a picture with me instead of going for a premade. Not naming the artist because thinking back the shop was not the cleanest and he was not the professional I expected everyone with a tattoo shop would be.
So after a while my bat due to aging started to look even worse, I started to call him Quasimodo because that's how his face looked like. I then found a really awesome Tattoo-shop in Hamburg, St. Pauli, Tattoostudio Dänemark where Frantz took care of the actually desperately crying me. I had some papers with different ornaments I drew with me and asked him to cover Quasimodo and to also tattoo the 'bit of shaved' part on the right side of my head. He did so on Halloween (I think it was 2007?) Those guys I can really recommend, Their shop is located next to the Reeperbahn and the guys are entertaining and do a great job and their shop is as clean as it should be! I also got another tattoo there but that will have to wait for another post.
But as time went by, as said, my sidecut was expanded and I got sick of the empty part above the tattoo so I went to a – back then – local artist (Ted, Surf Ink Tattoo, Kamen) to get a bit of ink added in 2013. I like his art and it always was nice to be there but actually getting an appointment via phone/online is a mess (and that's what I do when I live 3hrs away.) and communication seems to stop in a heartbeat. The right picture below was taken by Karsten Uwe Meyer.
That was the story so far. As mentioned in the beginning I again had some empty space. This time I went to a local artist in Wolfenbüttel, Melek from Memories Tattoo. This time I did not only want something to match the existing tattoo but also something that will match the future tattoo on the other side of my head (I see, it is getting cunfusing now, sorry.) So a spider with web it would be. Melek prepared a black widow and I agreed.
Took an hour and there it was, I really do like it but I think when it is healed we need to do something to make the spider stand out a bit more – which probably will be difficult since I don't want to have any color (maybe a hint of orange or green but nothing else) and I want to stick to 'something that looks like it's been drawn on my skin' instead of 3d/realistic looking stuff.
What kind of tattoos do you have? Any new plans? Have a nice evening!


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|Cooperation in the field of statistics
Every year a statistical programme is developed as a subset of, and in parallel with, the annual work programme elaborated by the European Commission. Statistical information from EFTA States is transmitted from them to Eurostat for storage, processing and dissemination.
Delivery process or content of this obligation overlaps with another reporting obligation ( http://rod.eionet.europa.eu/clients/7)
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|Data, Compliance, Text report


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Product concept
The current products related to intelligent life, wisdom and safety, wisdom and health-related areas, indoor positioning navigation, information data collection push, analysis and so on.
The company will focus on the development of micro-credit platform, the real realization of people and things, between objects and things interoperability, argumentation \"all things are coordinates, all things are spiritual\" theoretical basis.The company offers a full range of scenario application solutions based on a variety of needs. As well as a series of programs supporting the implementation of equipment.


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I tossed and turned last night thinking of the words that were uttered ......
Subtlely it was being put across trying to hide the meaning, but I am not stupid and I know what it meant. I pretended not to understand and said I'm OK with the plans.
I wonder, when will it stop? When I become a housewife? When death hits anyone of us ? or When illness struck so repentance can seep in the heart ? God Forbid, I pray it does not come down to that.
Alhamdullilah, even with the test that befalls on hubby and me ever since we got married 3 years ago, we somehow still maintain our Iman and patience and in fact it has made us look at things at a different perspective. I respect hubby's views and he has supported my stand when I disagree.
Hubby and me, we do have a loving relationship and time and time again, I respect him for his patience and positiveness. Yes, we do bump into rocks in the garden, but usually communication helps and I realised that we have to give and take to make it work. When things are upsetting, Go back to our Deen. It is the light that will help us. Our Deen.. what Rasullulah (saw) has thought us.
Its been 3 years, and the test that we face, this particular test that Allah (swt) have sent us have not change 1 bit. In fact, I fear it will get worse.
There is this person in our lives that is nice on the outside to us but at times, this person trows a bomb and shake the peaceful life that we have.
This person talks behind our back and criticised us.
When it comes to parenting, we are criticised.
When it comes to buying Insurance for our son, we were criticised.
When it comes to putting our son in a playgroup, we were criticised,
When it comes to bringing our son to the mosque, we were criticised.
Basically, we were and still am being blamed for many things even though hubby and me are very happy with how we lead our lives and how we bring up our children and how we manage our finances and we try our best to be good people.
To us, our children are our PRIORITY, and if we have that bit of savings or extra money at the end of the month, it will be for our children to benefit. We also want to have a holiday fund, a 'tea-tarik and mee goreng' fund, a weekend fund asides from our normal responsibilities.
Being inflexible and only holding on to Principles will only cause much problems which have been proven time and time again in front of our eyes. ( when it came out the second time and the 3rd time will only be game over for them ).
Time and Time again, we forgave this person and offer our salams and slowly continue our lives as if it didn't happen, as if we were not hurt, as if it didn't affect us. In a different perspective, we cant blame this person 100%. There must be a reason whey we are being treated this way. We may have offended this person unknowingly. Allah (swt) knows best.
Being the emotional one, I often share my disappointments to hubby on why are other people so concern about the way we lead our lives when we are perfectly fine with it. Yes, if we need help, we will approach the wise. But when we ourselves are perfectly fine, why do people criticised our move. Ya Rabb, with the birth of my second child ( insya'allah ), please grant sakeenah and Rahmah in our family.
Whenever I tell hubby, its NOT Fair, he tells me Allah (swt) is Fair. Whenever I tell hubby I don't understand, he tells me to leave it in the hands of GOD. He is sad too, but he makes doa alot.
Yesterday, I loss some sleep thinking about how that one being can be so calculative as to count even the amount of gas. GAS, FOOD, even if its not being paid the correct sum for, Isn't it Charity and isn't Charity noble and brings one Pahala and Good Deeds and Isn't having more Good Deed what we all want in our book of Deeds which will ultimately help us in Akhirat when the scale is weighing our Deeds and Sins ?
Masya'allah...... I am really loss for words. OK Please...I wont talk about this person anymore...I just need to let it out. Let me stop now as I don't want to open one's Aib, but when I have written is for me to LEARN and Reflect.
You know, I used to be like that..well not so much to calculate gas, but I don't like to share my toiletries with my siblings. I have my own toiletries basket which I keep in my room. I hate it when people use my things without my permission and hate to use my savings when I have to on " rainy days ".
After I got married, and live a life together with my hubby, I learn to share and not culcalate as hubby is not a calculative person at all and he always tells me that if we calculate againsts humans, Allah (swt) will calculate against us and when Allah (swt) calculates the ni'mat that he gives us, it will put us in the WRATH of destruction.
So Alhamdullilah, I have changed. And while money, petrol , and time are precious, I try not to think twice when it comes to helping people as these wealth are all Duniya. What we all should think of is Akhirat. Insya'allah If we think of Akhirat, Allah the Merciful will take care of our Duniya. Anyway, its really hard to find pahala these days... so giving a lift to anyone in need of it does not kill me. In fact, personally, I feel good that Ive done a good deed.
My hope is that when I look back and read this post, it will remind me to never hold a calculative attitude towards my children, family members and friends. Especially to my son and future child. Insya'allah. Our children are our AMANAH.
While it is rightful for them to return the favours when we are old, we should not expect it. When we don't expect and leave it in the hands of Allah to give them guidance, we will be taken care off.. Insya'allah. Amiin.


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The EU Council's Maltese Presidency's progress report on the EU Commission's proposal for an e-Privacy Regulation reveals that while Member States are still preparing their national positions, some delegations are already asking for greater...
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Toni Pepperoni! Bergfest judge since… ever, now joins the Love in AM Division! Awesome!
Name: Toni Perrone
Date of Birth: 20.10.1970
Hometown: Düsseldorf
Division: AM (unsponsored)
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Name: Tim; Rebensdorf;
Date of Birth: 25.11.1996
Hometown: Esslingen/Stuttgart
Division: PRO
Sponsors: Volcom,Muckefuck Wheels,Own Skateboards [+]
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Division: AM (unsponsored) [+]


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Instructor: Tammy Crenshaw, Sarah Maki
Duration: 4 weeks
Price: $170Session/Register
An American Kennel Club endorsed title that promotes responsible dog ownership and good doggy manners.
The AKC CGC program provides an excellent foundation for training in other fun activities such as rally, obedience, and agility. Therapy-dog groups often have passing the CGC test as a prerequisite for entrance into site-specific programs, some insurance companies recommend CGC training, and an increasing number of apartments and condos are asking for CGC training for resident dogs.
Open to all dogs, regardless of breed! This is a 3-week prep class with testing for the certificate on the 4th week.
Our goal is to promote responsible and healthy dog ownership


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Camis stared up at the high ceiling as he lay on the ground. He'd narrowly avoided getting crushed against the bus after Gaer's sudden growth spurt, but he had been thrown out onto the hard floor of the garage.
Tilting his head back a bit, wincing as a sharp pain shot though him, Camis saw the colossal 35 foot form of the panther standing over them. He was so tall...was there any way he'd be able to get inside the library? Would they have to break into the building's walls to simply get inside?
Not wanting to lead on that he was badly injured, Camis quickly got up.
"Yeah...I'm fine, Savage...but how's Gaer going to get inside now?" Camis asked. He turned around to Gaer. "And are you doing alright?"


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J. Stephen Grooms is a lifelong resident of Conway, South Carolina. He earned a Bachelor's of Science in 2005 from the University of South Carolina. He received his Juris Doctorate from the Charleston School of Law in 2010, and has been a member of the South Carolina Bar since that time.
Grooms began his legal career in Charleston, SC, by working in a plaintiff's litigation firm which handled a variety of plaintiff's injury cases. He was involved in several complex civil cases, primarily in Construction Plaintiff's Litigation. In May of 2011 Grooms accepted a position with the Horry County Solicitor's Office. While working in the Solicitor's Office Grooms handled over three thousand cases, and took numerous cases to trial achieving favorable results on cases ranging from drug offenses to murder. Grooms was recognized for his leadership and trial results and was promoted to Senior Assistant Prosecutor. His experience in the civil field and as a criminal trial attorney provides the knowledge to recognize the issues in your case, and the tools to represent you with confidence whether your case is criminal or civil in nature.
Grooms has been recognized for his achievements in the courtroom, but also in the community by the South Carolina's Prosecution Commission as well and the Horry County Solicitor's Office. Grooms married Ellen Hembree of North Myrtle Beach in 2015, and the couple resides in Conway with their chocolate lab Riley.


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Residential Home Inspection is a comprehensive and non-invasive inspection of all safety accessible systems and components of the home. We will check the condition of all systems and find problems or potential problems that buyers need to know about before purchase. During our inspection services, we inspect accessible areas including:
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HERE we have it again, the issue of whether a person deemed by the prosecutors to have commited an offence should be charged.
This time the person concerned is Mohamad Sabu, a.k.a. Mat Sabu, the Pas No. 2 who went on an adventure back into history about who's the good guy and who's the crook during the country's troubled times 61 years ago.
For picking out a certain Mat Indera as the hero instead of the 25 cops at the Bukit Kepong police station who were killed in a fire-fight, Mat has been charged with criminal defamation. Wrong call by the prosecutors?
Herein lies the dilemma. Countless reports were lodged with the police against Mat by all kinds of characters and NGOs. So the police had to investigate, right? Having completed this, the police must have concluded that Mat had committed an offence. Off the investigation papers went to the Attorney-General's Chambers, which decided that Mat had in fact done wrong. But this is not the end of the story because eventually it will be for the court to decide, whatever the opinions of the police and the AG.
This is how the system works in this country and a lot of people know this, certainly the legal trained.
But now some are saying that Mat should not be charged, especially since the prime minister had only very recently announced plans for more liberalisation and that after the ISA is repealed and replaced by other laws, no one would be punished solely for having dissenting political views. Correct but the PM was being specific about provisions in the new legislation. Other laws covering other offences will remain. Those questioning the wisdom of charging dear Mat must be able to draw a line somewhere. If they feel that what Mat said didn't deserve a charge, the way to go about it would be to ask that the law on this offence too be amended or repealed.
What everyone against the decision by the AG seems to have ignored is the need for the investigators and prosecutors to be truly independent, going about their work only guided by what the law says and provides for.
Using the political perspective to ask that someone is not charged only translates into interference in the work of the police and the prosecutors. Isn't this what many people don't like to see happen?