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JOHN McGINN is already acutely aware of the pressure to win promotion at Hibs.
The midfielder is on Scotland Under-21 duty this week but, just five weeks into his Easter Road career, has noticed the craving for a Premiership return among supporters.
Hibs were consigned to a second season in the SPFL Championship when they lost to Rangers in the play-offs in May. Since then, head coach Alan Stubbs has recruited heavily, with ten permanent signings and three loans.
McGinn is confident of achieving this season's aim and helping his club back to where he feels they belong.
"It is a Premiership club in the wrong division. It's down there for a reason," said the 20-year-old. "They got relegated because they weren't good enough.
"For me, it's about playing games every week. I had other offers which weren't guaranteeing that.
"It's a really important move for me because it's that age where I'm developing and I need to play to get better. There's an awful lot more pressure to win. I can feel that already but it's something I'm thriving on. It's a club that doesn't deserve to be in the division it's in."
The former St Mirren player held talks with Houston Dynamo during the summer before signing a four-year contract with Hibs. He explained how he didn't fully appreciated the size of the club until he arrived.
"I knew how good a club Hibs was but the size took me by surprise a little. You walk about the street and there are Hibees everywhere. I'm moving through next week so I'll have to start getting used to that. It's a privilege to play for such a big club. Hopefully we can bring the fans success."


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April 19, 2010
When you carry out acts of kindness, you get a wonderful feeling inside. It is as though something inside your body responds and says, "yes, this is how I ought to feel."
Kindness is an inner desire that makes us want to do good things, even if we do not get anything in return.
Volunteers polish up the rough spots in our communities by all that they do. They turn unheard of and thoughtless situations into precious treasured moments.
It is, and can be, a joy within our life to do some form or act of kindness. When we respond to this inner desire, there is kindness in everything we think, say, want, and do.
In other words, it's like saying, "Do all the good you can, By all the means you can, in all the ways you can, in all the places you can, at all the times you can, to all the people you can, as long as ever you can."
There are many people waiting for someone just like us to come along; people who will appreciate our compassion, our unique talents. Someone will live a happier life merely because we took the time to share what we had to give.
Too often, we underestimate the power of a touch, a smile, a kind word, a listening ear, an honest compliment, or the smallest act of caring.
All of us have the potential to turn life around. It is an overwhelming thought to consider the continuous opportunities there are within our day to make our love felt.
How far you go in life depends on your being tender with the young, compassionate with the aged, sympathetic with the starving, and tolerant of the weak and strong. Because, someday in your life, you will have been all of these.
A simple message: "A candle loses none of its light by lighting another candle; neither does any act of kindness."

subset: commoncrawl


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If Clients Don't Get It, It's You, Not Them
This time we hear from Larry Solomon, director of financial planning and investments at OptiFour Integrated Wealth Management in McLean, Va. He recounts an interaction that caused him to rethink his approach to communicating financial concepts.
A few years ago I had an eye-opening experience with our firm's client advisory board about my ability to communicate financial concepts to clients. At our firm we have a structured investment process we've been using since 2012. To help explain the strategy, I created a bunch of charts and slides that are included in client pitch books and presentations. But after a few years of using the slides, we started feeling that people were neither understanding nor embracing what we think of as our very unique way of managing money. We weren't closing as much new business as we thought we should and even existing clients didn't seem to appreciate, or could wrap their heads around, our strategy. Their questions made it clear we weren't doing a good job of explaining the benefits of our strategy.
We clearly weren't framing the basic concepts in a way that was digestible and made sense. I decided it was time to go back to the drawing board. I came up with some new ideas and created one of the best slides I thought we'd ever had on the topic of correlations. The main concept I wanted to get across with the chart was that correlation was not a good thing for portfolios.
I thought it was a beautiful chart and my colleagues agreed, so we presented it to our client advisory board. After the presentation, we were completely shocked by the response. While all our board members said they understood the concept I was trying to explain, they also said the presentation left them wanting more correlation, not less -- the exact opposite of the message I was trying to communicate.
It was embarrassing that I had totally confused this really bright audience. The way I was explaining the concept to them clearly didn't work. My colleagues, who had also thought my presentation was great, were similarly shocked that we had missed the mark by such a wide margin and communicated the exact opposite of what we wanted to.
It could've been tempting to stick with this slide I'd worked so hard on and explain away the board's reaction. Maybe they just weren't paying attention or they weren't smart enough. But I knew that wasn't true. Our board is made up of very, very smart people. We have a three-star general and industry leaders who are highly intelligent and educated people. The mistake was mine.
I thought about it a lot, trying to figure out where I went wrong and how I could make it right. Ultimately I decided not to try to communicate the idea of correlations at all because it's so counterintuitive. I just threw out the word "correlate." A lot of the jargon we use in our business makes sense to me and my colleagues, but people who aren't in our field don't get it. Jargon is a shortcut, ultimately -- but not an effective one if your audience doesn't get it.
It took me quite a few evolutions -- and almost a whole year -- before we finally nailed down an explanation that everybody was happy with. It was a lot of work, but ultimately we came up with something that our client board understood.
The bottom line is, if you explain something that the client doesn't understand, that's not the client's fault -- that's your fault. No advisor should ever say, "The client's not smart enough to figure it out."


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Monday, July 16, 2012
Who is that beautiful horse . . . don't you know how pretty I am
Now I realized when I started this trip, that of this pair, I would not be the one getting all the compliments. My girl is a lovely horse, if you like Arabs she is stunning and if you don't, she is stunning. So it came as no surprise to me when I was riding along, on my way to the Canby fairgrounds that people would stop, and tell me what a beautiful horse I had. It was an easy ride to the fairgrounds. Only about 15 miles from the pasture I had kept the girls in the night before, provided to us by our savior, Chia, after being refused entrance into Champoeg park. I was thrown for a loop there, having never been refused entrance into a state park before, but they were adamant that livestock(even lovely livestock) was not allowed inside the park. But we were rescued by our new friend, Chia, and after a restful night, Strip in a 40 acre pasture and us at Chia's house, it was on the road to Canby. I had gone about 9 miles when I was off walking for a bit and a bicyclist, who obviously didn't find Strip pretty, made some remark about it not being safe for me to walk along side a 55mph road. Being the ever so shy person that I am, I remarked that people were more likely to hit her, since they generally liked me and my horse and that "livestock"(lovely or otherwise) holds the right of way on the road. For some odd reason, she found that offensive and rode off in a snit. Reaching Canby with no other negative encounters, just loads of complements on my horse, I headed down an even busier road, Highway 99, into town. I had been riding about 3 blocks when Strip suddenly nickered. I leaned down and patted her neck softly, told her she was a good girl and then went back to watching traffic. A few minutes later, she did it again, I soothed her and looked around to see if she was worried about something I hadn't noticed and found nothing amiss. A third nicker and a slight look right and I began to worry myself. I looked right and saw nothing but an empty store front and I started watching her carefully. The next time she nickered and looked right I looked immediately, and saw our reflection. She was nickering at her own reflection in the windows as we passed them. I guess she must really be a beautiful horse to impress herself, since she has always been picky about what horsey company she keeps. Now I was heading towards the fairgrounds, to meet up with Jessica, she had tried to find us a place there, but 4H was having their annual horse fair and the lady running it, lets call her Schmanice, had told her everything was full but if she could find a place we were welcome to stay. She sent her around with her assistant to check out some possible spots, all unsuitable. So Jess was waiting for me out front. I arrived to find her not feeling well and to be greeted by one of the fair ground staff. The staffer was excited to have us there, she called her supervisor and after a call back it was decided to put us in the bone yard, not normally open to rental so not within the 4h woman's jurisdiction. We set up, Jess getting sicker by the minute and I ran to the store and returned just as she got sick. I think it was from the heat and the stress of trying to find a place. We were sitting there when Schmanice came zipping over in her golf cart, snapping at us sharply, demanding to know who gave us permission to be there. I told her who, just as the staffer arrived to back me up. Another staffer arrived, apparently Schmanice had been raising a stink with the fair grounds management about us being where we were, having not approved our existence herself, and the new staffer told us they were relocating us to behind the rodeo arena, once again a place not open to be rented. This seemed to annoy the poor representative of all that 4h stands for even more and she took off in her golf cart again. We pulled our camp, moved to the new digs, where they kindly set up a pen for Strip, and proceeded to plan our next few days. The next morning I was off to try and get some errands done and hadn't been gone 20 minutes when Jess called me. She had been given a heads up by one of the staff, someone, you guess who, had told the Fair manager that we had a rescue horse and it was obviously diseased and they were sending over a vet to check it out and see if we had papers for it. That was it for me. I turned around and headed back. Upon returning, I called the OSU Extension for 4H and asked if I was breaking any of their rules by being there. The kind lady on the phone said no and told me she would have the head of Oregon contact me. I then called the manager of the Fair Grounds to apologize for all the trouble I had brought her and to tell her that my horse, who I have had for 16 years was fully papered, health cert and coggins and I would be happy to get them copies. Then I explained to her what I was doing and why and told her that I would of course relocate, to which she said no, I was welcome to stay another day. I thanked her profusely and as I was hanging up the call from Doug rang through, I missed switching to it, new phone and all, and called him right back. He missed my call and soon recalled me. He was a very kind man, I explained my situation to him, telling him exactly what I was doing and wanted to make sure I wasn't violating any rules by being there. We had a long talk, about 4H and what they stand for, helping young people learn about animals and care and so much more, I was really quite surprised at the hostility I was receiving from this particular Schmanice person. He assured me there was no reason for my not being there and if the Fair grounds had placed me somewhere not related to the horse fair, then he couldn't see what the big problem was, laughing, he said maybe she was just over stressed with the fair. Personally, I think she was just lording it over her little kingdom and was mad because I went around her, but she might be in for a bit of surprise, since Doug was planning on having a talk with her. Either way, to call my horse a diseased rescue horse was a nasty low thing, I mean, look at how pretty she is.
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With Melbourne Cup around the corner, many of us are thinking about what makes for a sure bet and how to ensure you're a guaranteed winner. Just like trying to pick the winning horse, it is important to choose the best supplier for all your business needs. Whether you work in a warehouse, at an office or in the construction industry, you can bet on a sure thing with Signet! Offering the best packaging, safety and cleaning supplies in the country, Signet is proud to be the first choice for thousands of businesses – and here's why:
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No more ARTILLERY PIECES will be accepted at this event. You will be mailed a refund if you try and register anyway. You may still register participants.
Artillery Commander for the 150th Anniversary Battle of Shiloh will be Col. Robert Beams, Cleburne's Division, Blue Gray Alliance
Confederate Artillery Commander
Col. Robert Beams, Cleburne's Division
Federal Artillery Commander, Col. Boo Hodges
150th Anniversary
Battle of Shiloh
Registered unit information has been moved to a sub page. To find the sub page place your cursor over the ARTILLERY listing at the left and then click on the smaller box that appears to the immediate right.


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When incapacity strikes a person who has been handling the bill-paying or investment decision-making in a household, the finances of everyone in the household could be at risk. Here are a few proactive steps to consider taking now to plan for this risk.
Prevent hassles with legal documents
Every adult should have these legal documents in place:
A living will. You specify in advance what types of medical treatment you do or do not wish to receive in the event you become unable to make such decisions on your own.
A health care power of attorney. You use this to appoint someone to make medical decisions on your behalf in the event of incapacity. This enables the person you designate to act in circumstances in which the living will does not specify your wishes.
A financial power of attorney. You appoint a trusted person to make financial decisions on your behalf in the event of incapacity.
A will. This outlines how your assets will be distributed after your passing. If you have minor children, you can use a will to name a guardian. A will also directs how debt, taxes and expenses are to be paid and names an executor to file tax returns and manage your estate until everything has been distributed.
Explore a revocable trust
A revocable trust is a legal instrument you can use either together with, or in place of, a will. You transfer assets to the trust and specify how the assets are to be distributed after your death. Legally speaking, the trust owns the assets, but you can use them yourself for any purpose. You can terminate or amend the trust at any time.
Compared to a will, a revocable trust is generally harder to contest after your death. And because it doesn't become public record, a revocable trust keeps your personal affairs private.
Keep documents organized
Store essential legal and financial documents in a safe place, such as a fireproof, waterproof and portable box in your home. Tell someone you trust where the documents can be found.
Documents to store include, but are not limited to, copies of your will and other legal documents discussed above, insurance policies, deeds, mortgage papers, vehicle titles, and stock and bond certificates.
Keep an up-to-date master list of bank and investment accounts, retirement assets, real estate, debts, insurance policies and financial or investment advisors along with any usernames, passwords, personal identification numbers (PINs) and contact information required to gain access to more details about the items.
Consider long-term care insurance
With life expectancy on the rise, chronic diseases like diabetes and Alzheimer's are increasing the need for long-term care, services to help a person carry out essential, daily activities like eating, bathing and dressing. The high cost of long-term care can put your financial security at risk. Employee health insurance doesn't cover long-term care and generally, Medicare doesn't either. Medicaid does cover it, but only after the patient has spent or transferred away almost all assets other than a home and car.
Long-term care insurance is available to help defray the costs of such care, so you should consider such protection, particularly as you near retirement. This insurance can be costly. In many cases, the younger you are when you take out a long-term care policy, the lower your annual premiums will be.
Peace of mind from planning ahead
Incapacity isn't pleasant to think about, but being prepared is an essential part of financial planning. Knowing that you've taken steps to safeguard your finances from the risk of incapacity can help provide you with peace of mind, particularly as you move closer to retirement and then continue through that life stage.


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We partner with our clients, forming a relationship that allows us to go beyond understanding, so that we can develop the value of our client's products and effectively communicate this to the relevant bodies.
Our team consists of a diverse group of senior scientists, each with over 10 years of experience and with a professional background in either the pharmaceutical industry, consulting, public health or academia. A typical project team captures all this experience, routinely including a company partner, health economist, physician, senior scientist, medical writer and medical editor.


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Original Air Date: December 24, 1984
Gobo is having trouble believing in the Fraggles' Holiday traditions. Is he willing to risk their lives for his curiosity?
Fraggle Rock premiered on January 10, 1983, as a joint venture among many channels and many nations. Following the worldwide success of Jim Henson's Sesame Street, but also the difficulty translating the show to all the nationalities who wanted a piece, Fraggle Rock was designed to be a show easily understandable to all audiences.
The Fraggles dealt with universal themes like fear, jealously, sharing, and other issues easily relatable to viewers regardless of language or cultural background. So while US audiences tuning in on HBO saw sequences starring Doc, Sprocket and Uncle Traveling Matt, these brief scenes were re-shot to be made specific to the country it was airing in.
Fraggle Rock aired for only five seasons, yet has shown remarkable longevity on cable and abroad. Though the Henson Company has sold the rights to both The Muppets and Sesame Street, it retains ownership of The Fraggles, and is even readying a movie for them in 2010.
Oh, how I love Fraggle Rock! Each of the five characters is instantly lovable, and relatable to any human with a brain capable of watching. I was too poor to have watched these episodes when they originally aired in the states on HBO, yet I wasn't ready to fully appreciate it during the subsequent airing on TNT I was eventually exposed to.
Wembley is very committed to that tropical shirt
My preteen brain was unable to grasp how well written the show and its above-and-beyond music were, and only as an adult can I truly exalt the wonderful way in which the marvelous universe of Fraggles, Dozers, and Gorgs presented some incredibly deep and heartfelt lessons.
Doc explains to Sprocket the various cultural traditions people celebrate during the Winter Solstice
I dare say I could've used a viewing of The Bells of Fraggle Rock as a teenager, when I was so fucking busy screaming about the stupidity of religion and the inherent benefits of atheism. Seriously, this is a Christmas episode with a message so powerfully unique that I'm embarrassed about the way I conducted myself into near-adulthood.
FYI, The Fraggles never even mention Christmas
In this episode, Gobo, the adventurous young explorer, is beginning to question the cultural traditions of his fellow Fraggles. Every year, the Fraggles celebrate The Festival of the Rock. They dress up, sing songs and ring bells in order to trigger the chiming of the Great Bell and keep The Rock (their home, not the wrestler) from stopping at the apex of its orbit and making life uninhabitably cold.
Not feeling the group costume
Problem is, Gobo is finding it increasingly difficult to slap on a smile and perform the rituals his people have been merrily but blindly conducting for years in the name of fun and survival.
If a sad sack like Boober can get behind the Holiday, surely Gobo can?!
What is it all for, and how is anything they do making a bit of difference in the scheme of things? All of Wembley's, Mokey's, Boober's, and Red's promises of fun and costumes are doing little to persuade him to leave it be and just enjoy himself.
Bah Humbug
Even the phenomenal song "There's a Promise" (which I'm convinced should be allowed into our pantheon of Holiday music, if only for being absolutely beautiful and nondenominational!), lead by spiritual guru Cantus (who just so happens to be voiced by Jim Henson himself), does little to dissuade him.
Note the one guy not singing
It seems Gobo will only be satisfied with a literal interpretation of the Fraggles' holiday.
Gobo finds a map in a box marked "Really Old Stuff"
He must see the mythical Great Bell in the center of the rock for himself, or bring back word to the others that it was all a sham.
Cantus maintains he does not sound like Rowlf the Dog
Cantus counsels Gobo that he need not go on such a dangerous journey, since the answer he's really looking for is much closer. But Gobo makes a huge scene and embarks on the mission anyway.
Oh, and meanwhile Matt expounds on the strangeness of "Silly Creature" culture
Cantus quietly accompanies Gobo at a distance, and allows him to see whatever it is he needs to see.
I don't want to ruin it
Through a series of literal, as well as spiritual, interpretations, Gobo eventually does find the answer, even though it wasn't necessarily the one he was looking for.
Meanwhile, the gang freezes their asses off waiting for Gobo to return
But once he narrowly makes it back to Fraggle Rock, he finds that all of his friends have frozen. Whether this was due to the absence of Cantus or the lack of the ceremony is unimportant. The Festival of the Bells has not taken place, and Gobo has interfered with the natural order of things set in place by people far older and wiser than himself.
The horror!
Personally, I don't see it as a message of conformity, but rather one of cultural acceptance. The Fraggles' traditions are equal parts biological and religious; a fun way of acknowledging a balance within their universe.
Lesson hopefully learned
After unfreezing his friends, Gobo realizes that cultural traditions can have more importance than their face value. At no time is he asked not to question these beliefs, but he definitely learns that satisfying his own curiosity shouldn't have to come at the expense of the beliefs and behavior enjoyed by others.
Gobo joins in the reprise of this fantastic song
I'm not a religious guy by any means, but it's hard to interpret this any other way. Though neither Jesus nor Christianity are ever mentioned specifically, there's a profound message of cultural acceptance here and I believe faith is a big part of that.
The only Santa to be seen is the small toy that Sprocket plays with.
I genuinely feel that there's an unbelievably beautiful moral to The Bells of Fraggle Rock, one that encompasses all cultural holidays and truly embodies the spirit of the Christmas season better than just about any special I've ever seen. Doc does a good job of explaining to Sprocket why people celebrate winter holidays, and Traveling Matt's outsider perspective on the Human's Christmas really helps drive the point home. No Shit: If there's one Christmas special on this entire stupid blog I believe every parent should show their kids, it's this one.
PRODUCT INFORMATION (Support the Site!)
The Bells of Fraggle Rock appears on the one-disc Merry Fraggle Holiday, as well as on the boxed set, Fraggle Rock: The Complete Third Season. Of course, it's also contained in Fraggle Rock: The Complete Series Collection, but that should go without saying. I wholeheartedly recommend picking up any of these wonderful sets.
Yesterday's Christmas Special:


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- Research article
- Open Access
Histone acetylation associated up-regulation of the cell wall related genes is involved in salt stress induced maize root swelling
© Li et al.; licensee BioMed Central Ltd. 2014
- Received: 20 December 2013
- Accepted: 15 April 2014
- Published: 23 April 2014
Salt stress usually causes crop growth inhibition and yield decrease. Epigenetic regulation is involved in plant responses to environmental stimuli. The epigenetic regulation of the cell wall related genes associated with the salt-induced cellular response is still little known. This study aimed to analyze cell morphological alterations in maize roots as a consequence of excess salinity in relation to the transcriptional and epigenetic regulation of the cell wall related protein genes.
In this study, maize seedling roots got shorter and displayed swelling after exposure to 200 mM NaCl for 48 h and 96 h. Cytological observation showed that the growth inhibition of maize roots was due to the reduction in meristematic zone cell division activity and elongation zone cell production. The enlargement of the stele tissue and cortex cells contributed to root swelling in the elongation zone. The cell wall is thought to be the major control point for cell enlargement. Cell wall related proteins include xyloglucan endotransglucosylase (XET), expansins (EXP), and the plasma membrane proton pump (MHA). RT-PCR results displayed an up-regulation of cell wall related ZmEXPA1, ZmEXPA3, ZmEXPA5, ZmEXPB1, ZmEXPB2 and ZmXET1 genes and the down-regulation of cell wall related ZmEXPB4 and ZmMHA genes as the duration of exposure was increased. Histone acetylation is regulated by HATs, which are often correlated with gene activation. The expression of histone acetyltransferase genes ZmHATB and ZmGCN5 was increased after 200 mM NaCl treatment, accompanied by an increase in the global acetylation levels of histones H3K9 and H4K5. ChIP experiment showed that the up-regulation of the ZmEXPB2 and ZmXET1 genes was associated with the elevated H3K9 acetylation levels on the promoter regions and coding regions of these two genes.
These data suggested that the up-regulation of some cell wall related genes mediated cell enlargement to possibly mitigate the salinity-induced ionic toxicity, and different genes had specific function in response to salt stress. Histone modification as a mediator may contribute to rapid regulation of cell wall related gene expression, which reduces the damage of excess salinity to plants.
- Zea mays
- Cell enlargement
- Cell wall related genes
- Histone acetylation
- Root swelling
- Salt stress
Soil salinization owing to agricultural irrigation leads to crop growth rate reduction and yield decrease. The understanding of the mechanisms by which plants cope with high concentration of salt could enhance productivity in the high saline conditions. Excess NaCl inhibits plant growth both in shoots and roots [1]. A significant growth reduction in the maize shoot and primary root is observed following NaCl treatment [2, 3]. One reason of the growth suppression is inadequate photosynthesis due to stomatal closure and consequently limited carbon dioxide uptake under salt stress [4] and thus most morphological and transcriptional studies on the effect of excess salinity have been focused on shoots and leaves because they are responsible for photosynthesis. But the effect of this stress on roots should be more obvious as the root is the organ that is directly exposed to the salinity soil [5]. The molecular and cellular mechanism why the growth of young roots was repressed under salt stress is not precisely known.
The plant growth requires concerted water uptake and irreversible cell wall expansion to enlarge cells [6]. The mechanical character of the cell wall controls the cell size and shape through the governance of cell expansion, which determines the morphology of tissues and organs [7]. Several studies using transgenic materials have confirmed the role of expansins in promoting cell enlargement by affecting cell wall loosening [8, 9]. The plant cell wall is a dynamic network structure that consists of cellulose microfibrils and helicellulose embedded in a pectin matrix and contains proteins and numerous enzymes [10]. This structure is important in plant growth and development and in response to various environmental stresses [11]. The cell wall related proteins are believed to play a role in modulating cell wall extensibility that mediates cell enlargement and expansion. These proteins include xyloglucan endotransglucosylase (XET), endo-1,4-b-D-endoglucanase (EGase), expansins (EXP), and the plasma membrane proton pump (PM-H+-ATPase, MHA) [12]. The low water potential is found to increase XET activity in the apical region of maize roots [13], although the possible role of XET in cell wall extension could not yet be confirmed in vitro [14]. Expansins have been reported to induce immediate cell wall loosening in vitro and in vivo [15], and may be involved in acid-induced growth through disrupting the link between cellulose microfibrils and adjacent matrix [16]. The expansin gene family that shares conserved motifs comprises four gene subfamilies: α-expansin (EXPA), β-expansin (EXPB), expansin-like A, and expansin-like B [17]. The expansin gene expression level is highly related with the elongation growth of roots, internodes and leaves [8, 9, 18]. However, individual expansins are observed to be prior expressed in specific organs, which suggested that individual expansin genes had specific roles for plant development. PM-H+-ATPase can pump protons into the apoplast from the cytosol to acidify the apoplast where acidification activates expansin activity that in turn loosens the cell wall and expands cells [19]. Xyloglucan is the most common hemicellulose in the primary cell wall in most plants. XET has been proposed as a potential cell wall extension protein because XET is able to cleave and rejoin xyloglucan chains [20]. An up-regulation of the ZmXET1, ZmEXPA1, and ZmMHA mRNAs is found in maize shoots [12].
The gene expression is influenced by chromatin structure, which is dependent on epigenetic regulation, such as histone post-translational modifications and DNA methylation. The basic repeated unit of chromatin is the nucleosome in eukaryotes, which is formed by wrapping approximately 146 bp of DNA around a histone octamer that consists of two copies of each histone proteins, H2A, H2B, H3 and H4 [21]. The N-terminus tail (N-tail) amino acid residue of the histones, exposed on the surface of the nucleosome, is subjected to post-translational modifications, including acetylation, methylation, phosphorylation and ubiquitination, catalyzed by histone modification enzymes [22, 23]. Histone acetyltransferases (HATs) are classified into two categories based on their subcellular distribution: the type A HATs and the type B HATs [24]. Histone acetylation is regulated by HATs and often correlated with gene activation [25]. Histone modification is involved in transcriptional regulation of many genes under salt stress [26, 27].
An understanding of the growth response of crop roots at cellular and molecular levels to salinity is of fundamental importance for a better comprehension of plant resistance to excess salinity and the breeding of salt stress-adapted crops. The cell wall is thought to be the major control point for cell enlargement, which is related with plant stress response. Currently, little is known about whether the histone modification is involved in regulating the expression of the cell wall related genes under salt stress conditions. This study aimed to analyze cell morphological alterations in maize roots as a consequence of excess salt in relation to the transcriptional and epigenetic regulation of the cell wall related protein genes. Salt stress induced maize growth inhibition along with root swelling and cell enlargement, which were accompanied by an up-regulation in some cell wall related genes. The global histone acetylation levels of H3K9 and H4K5 were increased in treated seedlings and the transcript levels of the ZmHATB and ZmGCN5 genes were increased, which might be an adaptive response of plants to salt stress. ChIP results displayed that up-regulation of the ZmEXPB2 and ZmXET1 genes was associated with an increase in histone H3K9 acetylation levels on the promoter regions and coding regions of these two genes in response to salt stress. Our data indicated that salt stress-induced elevation of H3K9Ac was accompanied by the change of cell well related gene expression, resulting in an adaptive cellular and growth response.
High salinity causes the elongation zone swelling and the meristematic zone shortening
High salinity activates the expression of HATs and increases global histone acetylation levels in the genome
High salinity selectively affects the expression of the cell-wall related genes
The local H3K9Ac levels of ZmEXPB2 and ZmXET1were increased under high-salinity stress
High salinity inhibited root growth and resulted in cell enlargement and root swelling
A high concentration of NaCl reduced root growth in many crop plants [38]. In this study, 200 mM NaCl treatment caused maize growth inhibition, and the primary root length was significantly reduced. This was consistent with previous observations in maize and cotton seedling roots [2, 39]. The swelling elongation zone became wider and longer and the meristematic zone was reduced in length with the increasing of the treatment time with 200 mM NaCl (Figure 1B). Root swelling was also observed in maize roots exposed to salt stress [40] and aluminium stress [41, 42]. The formation of tuberized roots also has been reported in A.thaliana to be a consequence of drought stress [43] and salt stress [44, 45]. It has been reported the length of the meristematic zone of the primary root tips was reduced by 56% after 1 week of 1% NaCl treatment in A.thaliana. Our cytological analysis showed that the cortical cell radical enlargement after 200 mM NaCl treatment resulted in an increase in the root diameter. Similarly, a significant decline in the ratio of the cross-sectional area of the stele to area of the root was observed with increasing NaCl concentration in cotton roots [39]. It has been reported that a radical swelling of all cell layers in root tips of Arabidopsis thaliana after 2 weeks of 1% NaCl stress [44]. The length and volume of the cortical cells were increased, but the cell density in the cortex was significantly decreased, indicating that the cell production was decreased during salt stress. It has been reported that in cotton roots salinity diminished the rate of cell production [39]. Burssens et al. [44] reported that the inhibition of A.thaliana root growth with salt stress is at least partially due to a decrease of cell production. The stele tissue cells transport water and soluble mineral nutrients from the roots to shoots. The transverse and radical enlargement of stele tissue cells was first emerged on the longitudinal section and the number of stele tissue cell layers was increased both in the transverse section and longitudinal section of the roots. These may help cells uptake more water and create more barrier to reduce Na+ concentration, which may be an adaptive mechanism to defense ionic toxicity. A decrease in the meristematic zone length of the primary root and the elongation zone cell numbers may be the reasons why the root growth was inhibited.
High salinity induced cell enlargement and root swelling in the elongation zone are accompanied by up-regulation of some cell wall related genes
The expression levels of expansin genes were increased in response to submergence in deepwater rice internode [46]. The GmEXP1 expression level was very high in soybean roots where rapid root elongation took place and ectopic expression of GmEXP1 accelerated the growth of transgenic tobacco roots, which showed insensitivity to stress [18]. Excised stem segments treated with auxin rapidly increased cell elongation, and the mRNAs of EXPA1, EXPA3, EXPA4 and EXPA5 were increased within 1 h [47]. ExpB2 plays a role in the elongation of maize roots, and may be also involved in plant responses to environmental stimuli [48]. It has been reported that the expression of EXP1, EXP5, EXP6 and EXP8 genes was up-regulated in maize primary roots after grown at low ψw, which likely contributed to enhanced cell wall extensibility and thus helped root cells maintain elongation at reduced turgor pressure [35]. The expression of XET and expansins was about 100-fold higher in cotton fiber cells, corresponding to their proposed role in cell enlargement [49]. The transcription levels of expansins and XET were increased after salt stress [50, 51]. The XET activity was enhanced in the apical region of maize roots from plants grown under low water potentials, and was suggested to be necessary for maintaining elongation [52]. Our RT-PCR experiment showed that the transcript levels of ZmEXPA1, ZmEXPA3, ZmEXPA5, ZmEXPB1, ZmEXPB2 and ZmXET1 were increased from 2 to 96 h after exposure to high-salinity treatment. We presumed that the up-regulation of these five expansin genes and ZmXET1 was an adaptive mechanism to regulate the transverse and radical enlargement of the elongation zone cells, which may mitigate the decrease in root growth and the damage under high-salinity stress. It has been reported that the average cell length of mesocotyls was increased by up to 58% in the transgenic lines that overexpressed OsEXP4 [8]. The ZmEXPB4 gene was down-regulated after treatment with 200 mM NaCl. Hormone treatment induced expression of Exp1 but repressed that of ExpB2 in maize roots [30]. Therefore the differential expression patterns of expansins suggest that each expansin member may play a specific role in root growth and development, and in response to external stimuli. The acid growth theory thought that an auxin-induced acidic environment was needed for elongation growth [53]. PM-H+-ATPase can pump protons into the apoplast from the cytosol to create a acidic environment and thus the down-regulation of ZmMHA may reduce root elongation growth.
Histone acetylation may be involved in high salinity-induced gene expression regulation
Recent studies have revealed that gene expression is regulated by dynamic histone modification, which could be an important mechanism for plants to adapt to abiotic stress [31, 54]. In tobacco BY2 and Arabidopsis T87 cells, high-salinity and cold stress triggered rapid up-regulation of histone H3 Ser-10 phosphorylation and histone H4 acetylation that was correlated with activation of stress-responsive genes [27]. The mutations of GCN5 and ADA2 that encode the components of histone acetyltransferase complexes affected the expression of the cold stress-responsive genes in Arabidopsis [55]. The enrichment of H3K9 acetylation and H3K4 trimethylation was related with the up-regulation of RD29A, RD29B, RD20, and RAP2.4 genes in response to drought treatment [30]. Similarly, salt stress enriched H3K9K14 acetylation and H3K4 trimethylation on the promoter and coding regions of DREB2A, RD29A, and RD29B [26]. Our results showed that the total acetylation levels of H3K9 and H4K5 in the genome were increased after treatment with 200 mM NaCl and this increase could be associated with the enhanced expression of ZmHATB and ZmGCN5. Therefore overall histone acetylation level change is likely to be an adaptive response to salt stress at the epigenetic levels. It has been reported that the overall acetylation level alteration may be related with basal transcription and help rapid restoration of the acetylation level when the recruited HAT is removed [56]. Our results diaplayed salt stress caused the up-regulation of ZmEXPB2 and ZmXET1 genes, which was accompanied with the elevated H3K9 acetylation levels on promoter regions and coding regions of these two genes. These data support the conclusion that epigenetic regulation plays a vital role in rapid regulation of gene expression in plant adaptive response to environmental stimuli [57, 58].
This study showed that the stele tissue and cortex cells were enlarged after treatment with 200 mM NaCl, which was associated with an up-regulation of cell wall related genes ZmEXPA1, ZmEXPA3, ZmEXPA5, ZmEXPB1, ZmEXPB2 and ZmXET1. The expression of histone acetyltransferase genes ZmHATB and ZmGCN5 was increased accompanied by an increase in the global acetylation levels of histones H3K9 and H4K5, suggesting that epigenetic regulation was involved in salt stress response. ChIP experiment further indicated that the up-regulation of ZmEXPB2 and ZmXET1 genes was associated with the elevated H3K9 acetylation levels on promoter regions and coding regions of these two genes. These data imply that an epigenetic control of the expression of the cell wall related genes in response to salt stress results in cell enlargement and root swelling which is an adaptive response.
Plant materials and treatments
Maize seeds (Zea mays L. hybrid line Huayu 5) were germinated in the dark at 25°C on cotton gauzes soaked in water on the glass dish, and then the seedlings of uniform size were transferred to hydroponic cultures in buckets containing 1/2 Hoagland's nutrient solution in a controlled environment chamber under relative humidity of 70%, photoperiod of 14 h irradiance of 120 μmol m-2 s-1 with temperatures of 25°C and in the dark 10 h of 20°C respectively. The solutions were fully renewed every 2 days. After 6 days, when the seedlings with two leaves, 200 mM NaCl was added to nutrient solution to initiate the saline treatment. Six-day-old maize seedlings grown in 1/2 Hoagland's nutrient solution without NaCl were considered as a control group.
Growth measurement
Six-day-old maize seedlings were transferred to nutrient solution supplemented with 0, 25, 50, 100, 150, 200 and 250 mM NaCl respectively for 7 days, then the image was obtained by Nikon J1 (Nikon Corporation, Japan). Six-day-old maize seedlings (n = 20) were transferred to nutrient solution supplemented with or without 200 mM NaCl treatment for 0, 2, 4, 8, 16, 24, 48 and 96 h, then maize seedlings were photographed by Nikon J1 (Nikon Corporation, Japan) and the primary root length and plant height were measured by Image J.
Root swelling and Feulgen staining
Feulgen staining of the primary roots was performed on 20 maize seedlings after 24, 48, 72 and 96 h treatment with nutrient solutions containing 0 (control) or 200 mM NaCl. Primary roots were fixed over night in a solution of ethanol and glacial acetic acid in a 3:1 ratio. Subsequently, roots were washed several times with 70% ethanol, followed by a gradual rehydration in increasing ethanol concentrations, 5 min per step with three changes of water at the end [59]. Hydrolysis was performed in 1 N HCl for 15 min at 60°C, and stopped by replacing HCl with water. Root staining was achieved for 1 h in the dark at room temperature with Schiff's Solution (Sigma, Taufkirchen, Germany) [60]. After 1 h the roots were washed three times by deionized water and examined by Stereo Microscope (China) with 10X objective and 0.8X ocular. Images were captured by IScapture software (ISC, China) with a CCD monochrome camera (TCC-5.0, China).
Light microscopy
For light microscopy studies, after a short rinse (10 s) with distilled water, the tips (0–10 mm) from primary roots were excised from control and 200 mM NaCl treated seedlings after 48 h and 96 h of exposure to salt treatment. The samples were immediately fixed with 3% glutaraldehyde and post fixed with 1% osmium tetroxide, dehydrated in ethanol series followed by embedded in Spurr's resin. The transverse sections at approximately 5 mm from the apex and the longitudinal sections between 0 and 3 mm from apex were cut by ultramicrotome. Semi-thin transverse and longitudinal sections were stained with methylene blue (MB). Methylene blue stained specimens were examined with an Olympus BX-60 fluorescence microscope (Olympus, Tokyo, Japan) with bright-field illumination at 4X and 10X. Images were captured with a CCD monochrome camera Sensys 1401E and processed with ADOBE PHOTOSHOP 9.0 software (Adobe Systems, San Jose, CA).
The nuclei of maize roots were prepared in the slides according to the reported method [61]. Immunostaining of the nuclei on the slides was carried out as described by Zhang et al. [62]. The primary antibodies were H3K9Ac (catalog number: 07–352, Millipore, Billerica, MA, USA) and H4K5Ac (catalog number: 06–759, Millipore, Billerica, MA, USA) and the secondary antibody was fluorescein conjugated goat anti-rabbit IgG (catalog number: 12–507, Millipore, Billerica, MA USA). In control experiments, slides were incubated with the secondary antibody only. All slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI, Sigma, USA), mounted with Vectashield (Vector labs, USA). Images were captured with a CCD monochrome camera Sensys 1401E under an Olympus BX-60 fluorescence microscope with filter blocks for DAPI and fluorescein, then pseudo-colored and merged using the software MetaMorph 7.7.2 (Universal Imaging Corp., USA). Microscope settings and camera detector exposure times were kept constant for the control and treated groups and more than 300 nuclei were analyzed. Images were processed using ADOBE PHOTOSHOP 9.0 software (Adobe Systems, San Jose, CA). The mean gray value of the immunostaining signals for H3K9Ac and H4K5Ac in the control and NaCl-treated samples was measured with Image J and MetaMorph. For both the control and treated groups, three independent immunostaining experiments were performed with each antibody. Mean gray value of the signal intensity and standard error of the mean value were calculated with SPSS10.0 for Windows package (SPSS Inc., 1999).
Western blot assay
Proteins were extracted from maize seedling roots by grinding in the liquid nitrogen and resuspended in the extraction buffer [100 mM Tris–HCl pH 7.4, 50 mM NaCl, 5 mM ethylenediaminetetraacetic acid (EDTA) and 1 mM phenylmethanesulfonyl fluoride (PMSF)]. Western blot detection was carried out as described by Yang et al. [63]. Proteins were fractionated by SDS-PAGE and transferred to Immobilon-P membranes which were respectively incubated with the primary antibodies H3 (catalog number: 06–755, Upstate, Lake Placid, NY, USA), H3K9Ac and H4K5Ac overnight at 4°C. Detection was performed using alkaline phosphatase (AP) conjugated anti-rabbit IgG antibody and chemiluminescence visualization. Histone H3 was applied as an equal loading control. Densitometric measurements were taken after immunodetection using Image J. Abundance index was calculated as follows: H3K9Ac or H4K5Ac band intensity/H3 band intensity. Western blots were repeated three times for each sample from three independent experiments. Mean abundance index and standard error of the mean were calculated with SPSS10.0 for Windows package (SPSS Inc., 1999).
Quantitative real-time PCR
RT-PCR primer pairs for maize (Zea mays L.)
Sequence (5'-3')
Chromatin immunoprecipitation (ChIP)
ChIP-PCR primer pairs for maize (Zea mays L.)
Sequence (5'-3')
Product length (bp)
ZmEXPB2 SetA
ZmEXPB2 SetB
ZmEXPB2 SetC
ZmEXPB2 SetD
ZmXET1 SetA
ZmXET1 SetB
ZmXET1 SetC
ZmXET1 SetD
Accession numbers
The accession numbers for the genes described in this paper are HATB [GRMZM5G851405_T02], GCN5 [GRMZM2G046021_T01], XET1 [GRMZM2G026980_T02], MHA [GRMZM2G148374_T01, GRMZM2G019404_T02, GRMZM2G450055_T01, GRMZM2G006894_T01], EXPA1 [GRMZM2G339122_T01], EXPA3 [GRMZM2G074585_T02], EXPA5 [GRMZM2G019398_T01], EXPB1 [GRMZM2G146551_T02], EXPB2 [GRMZM2G021621_T02], EXPB4 [GRMZM2G154178_T01], and Actin [GRMZM2G126010_T01].
We thank the anonymous reviewers for critical reading and constructive suggestions on improving the quality of this manuscript. This work was supported by the NSFC (No. 31171186) and Hubei Province Natural Science Fund.
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- Luo M, Liu X, Singh P, Cui Y, Zimmerli L, Wu K: Chromatin modifications and remodeling in plant abiotic stress responses. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms. 2012, 1819 (2): 129-136. 10.1016/j.bbagrm.2011.06.008.View ArticleGoogle Scholar
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


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via: hanneli, late afternoon, joujouvaleroy, unknown
You might know that I am a huge lover of pastel colors: light pink, mint, salmon, liliac are all part of my wardrobe. I had some problems with baby blue, but I am starting a relation ship with it too.
Right now a fashion favorite is LEMON CANDY YELLOW.
It's a big big love! Especially in combination with all shades of gray and other pastels. Fashion weeks are full of it and I have to get me some pieces quick. What's about you, like it or hate it?


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40 Best Ways to Lose 5 Pounds ...
These Best Ways to Lose 5 Pounds don't need to tax you, take up a lot of time, or turn you into a gym rat. There are tons of ways to lose 5 pounds, and you don't have to be unhealthy about it, either. Forget crash diets, fads, fake weight loss shakes, or diet pills. If you need to shed 5 pounds, you can do so quite easily just by changing up your daily routine. It all comes down to making smart choices, eating right, and getting more active during your day to day life. I've compiled a list of the very best ways to lose 5 pounds and by following these tips, you'll be able to shed those excess pounds in no time!
1. Know, then Control, Your Portions
Portion control is one of the most excellent ways to lose 5 pounds, and it doesn't really require any extra effort on your part. Many experts suggest actually weighing your food, measuring everything, et cetera, at least once a week. If you have the time and patience to do that, go for it. However, there are simpler methods to follow as well. For instance, eyeball your portions; typically, sides like starches should be about the size of your palm, meats should be fist sized, and you can generally eat as many vegetables as you like, as long as they aren't cooked in an unhealthy manner.


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Jedi Gang Fight
The citizens of Flint are no stranger to gang fights. But for as violent as our city is, I don't think there has ever been a Jedi gang fight. Check out the nerdy violence.
You've gotta respect Star Wars nerds, even if they would trade an inch off their wiener to have a real light saber. Star Wars nerds come from all walks of life and for the most part are a very proud people. The movies are pretty alright, but if you have a Princess Leia fetish, you really need to grow up.


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Manor.Govfresh was a fantastic experience. Innovators from all over America shared and learned about better ways of leveraging new technologies to benefit cities and citizens. Great minds from small towns, creative ideas from industry gurus, and plenty of friendly networking made for a productive weekend -- SeeClickFix was honored and excited to be part of it. But you don't have to take our word for it -- this nifty video was published by Manor New Tech High, which they put together to capture attendees' thoughts and insights.[youtube http://www.youtube.com/watch?v=IspDhQCm9V0&fs=1&hl=en_US]
Guess who scored a quote in the video about open government and communication -- our own Kam Lasater, who represented SeeClickFix at Manor. Kam mentioned how great it was to see the concepts of Gov 2.0 getting talked about: "It's not that open government is new, or that Gov 2.0 is new," he commented. "It's that those conversations were always happening -- and now we're seeing them published everywhere."
Preach it, Kam -- open government means accountable, accessible government. And that's what SeeClickFix is all about.


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My Experience
"they might as well give you a gun and say "go kill yourself" because thats what this is going to do."
Great post Chris.
I was heading to the shore with my friend and wanted to grab something at WaWa to eat for the day. I was pretty surprised that I was able to find enough stuff to hold me over. I ended up getting
2 Hard boiled eggs
Bag of Beef Jerky
Trail mix (without peanuts)
I'll take your version of the double down, extra avocado, to go please.
My fast food faves...
chipotle- order the salade (minus rice & beans) add guac, salsa, peppers and onions and your choice of meat. i skip the dressing all together the salsa and guac does the trick. They claim their meat is "natuarally raised" Chipotle rocks!
Pizza joints- get a steak,chicken steak or roast pork without the roll...add spinach or Broccoli rabe (my fav!)
Whole foods- all ingredients listed in the hot foods bar, there is a lot that isn't paleo but there are options. the new store in Plymouth Meeting has a huge hot food section.
I like eating out...and do prob once a week. Its challanging at times but it is possible.
Ditto that Chipotle rocks. I do the same thing, order a salad with avocado and skip the rice and beans.
In College Station we have some pretty good barbecue places. I'll do sliced beef, no sauce, green beans and cole slaw, and skip the bread.
If I were to answer your poll,I would go for C.lol.ReplyDelete


text: Plot Summary:This horrific tale begins in 1983, when shortly after coming across a magical ancient artefact, Navy pilot Hank Campbell loses his mind and murders all his fellow pilots. Right before getting locked up, he hides the powerful object somewhere in the plane. 'Blood Relic' tells the harrowing story of what happens more than two decades later, as preparation of the aircraft for an air and space museum exhibit coincides with Hank's release from a mental institution. With the knowledge of where the talisman is hidden, Hank sets out to wreak voodoo havoc on the people who put him away.


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- Sarah Burke-Spolaor (National Radio Astronomy Observatory)
- Gravitation
- Gravitational waves
- Galaxy mergers
- Fundamental physics
- Black hole physics
- Surveys
- Pulsars
- Instrumentation
- FM14.1.01 Marta Volonteri: Major Questions in the Evolution of Galaxy and Black Hole Mergers
- FM14.1.02 Allison W. S. Man: The Role of Galaxy Merging in the Life of Massive Galaxies
- FM14.1.03 Eva Martinez Palafox: Supermassive Black Hole Binaries: Environment and Galaxy Host Properties of PTA and eLISA sources
- FM14.3.03 Laura Blecha: Modeling the Observability of Recoiling Black Holes as Offset Quasars
- FM14.3.04 David Roberts: X-Shaped Radio Galaxies and the Gravitational Wave Background
- FM14.7.03 Peter Bender: Comparison of Laser Interferometry and Atom Interferometry for Gravitational Wave Observations in Space
- FM14.7.02 Robin Stebbins: Status of a Space-Based Gravitational-Wave Observatory at NASA
The topics of this Focus Meeting are at the intersection of galaxy evolution and fundamental physics---how studies of galaxy mergers can inform the detection of gravitational waves and how the detection and study of gravitational waves can constrain models for galaxy mergers.
The merger of galaxies is now recognized as an integral aspect of galaxy assembly and evolution, with large galaxies in the local Universe potentially having undergone multiple mergers. Most, if not all, large galaxies in the local Universe have supermassive black holes (SMBHs, > 10^7 M_sun) in their nuclei. A robust prediction of the galaxy merging process is that the merger product will contain two SMBHs, which, through dynamical friction, will sink to the center of the merger product. The two SMBHs form a bound binary when their separation is of order 10 pc. Further dynamical friction by stars in the central region, and possibly gas interactions, should cause the binary to harden, eventually to the point at which gravitational wave emission dominates its dynamics.
The broad overview of galaxy mergers and gravitational wave emission sketched above has received considerable support over the past decade. Simulations of merging galaxies have grown considerably in both complexity and reality, with circumbinary (SMBH) gas accretion disks and asymmetric stellar velocity distributions being incorporated. An important result of this recent work is that a potential bottleneck---often termed the "last parsec problem," in which the two SMBHs could not lose enough energy to approach closer than 1 pc and would never emit gravitational waves---now seems much less formidable. While specific hierarchical structure formation scenarios are even making quantitative predictions about the fraction of galaxies (~ 0.1--1%) of galaxies that might harbor dual SMBHs at redshifts z < 2, there are also substantial uncertainties in aspects such as the dynamical friction time scale.
Accretion onto the dual SMBHs should power a dual active galactic nucleus (AGN), and determining the rate of dual AGN formation (or the space density) is an important constraint on models. Observationally, there have been a variety of multi-wavelength approaches (X-ray, visible/IR, and radio) taken to search for and identify both dual AGN and, in some rare cases, there have even been proposed binary SMBHs found. While the spatial density of dual AGN at kiloparsec separations (~ 5 kpc) is becoming increasingly well constrained, there are only a few systems known at late (< 1 kpc separations) and very late (< 100 pc separations) stages of merger. These very late stages are of the greatest interest due to the increased interaction and certainty of their eventual coalescence at these late stages.
Excitingly, recent progress in pulsar timing arrays---constructed from a set of precisely timed millisecond radio pulsars---are beginning to encroach upon the gravitational wave strain amplitudes predicted from some of models for galaxy merger rates and cosmological structure formation. International consortia, using some of the world's largest radio telescopes, are obtaining sensitivities at nanohertz gravitational wave frequencies---frequencies at which in-spiraling SMBHs should be emitting. On the time scale of the General Assembly, all three of the international consortia should be approaching strain sensitivities such that the probability of a detection could exceed 90%. Much of the initial focus for pulsar timing arrays has been on the stochastic background, produced from the ensemble of in-spiraling SMBHs. There has also been considerable recent progress in understand the extent of the source population for pulsar timing arrays, and, by the time of the General Assembly, there should be robust pipelines searching for individual SMBH binaries.
The recent decision by ESA for a focus on the Gravitational Universe also provides a path toward the detection of millihertz gravitational waves, which should be emitted by in-spiraling SMBHs at an even later stage in their evolution. The absence of a detection by one or more of the international pulsar timing array consortia could potentially influence the design of future space-based missions.
Finally, studies of dual AGN systems may be able to identify common features in recent galaxy mergers that are easy markers for later stages of galaxy mergers. If so, observations and simulations of merging galaxies may inform attempts to "tune" gravitational wave telescopes, even enhancing the probability of the first gravitational wave discovery.


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The Chicago Bar Association Symphony Orchestra and the CBA Chorus: Chicagoland's unique musical ensemble of attorneys, judges and legal professionals, founded in 1986. David Katz, music director and principal conductor; Stephen Blackwelder, CBA Chorus director; Marek Rachelski, resident conductor, CBASO
Sunday, August 26, 2012
2012-13 SEASON and SINGLE TIX on sale NOW!
TICKETS online: brownpapertickets.com/profile/80112
SEASON PASS online: best savings, plus reserved seating
INDIVIDUAL TICKETS in advance: $12 online adults/$10 students (plus tiny processing fee) or from any ensemble member!
TICKETS: $15 adults/$12 students at the door
Although there are no refunds, you may exchange tickets for another performance. Simply bring your unused tickets to the ticket table at St James on the day of the performance you wish to attend.
Scroll down to read more about our 2012-13 season of concerts at St James Cathedral.
Subscribe to: Post Comments (Atom)


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HTML5 makes the world much better. At least in general.
One of the nice features of HTML5 is the possibility to give input fields a type-attribute, which helps browsers determine what kind of input the field is supposed to accept. The Browser can then use this info to both validate input and suppress form submission if the input doesn't match, but also to offer some additional help for input of valid data. This is especially nice for mobile devices where the device can switch to an appropriate keyboard or show some fancy date input widgets.
The latest nicety I came across was the combination of jQueryUIs datepicker and Chrome's datepicker when a text input is defined as type="date".
This exact combination causes several problems:
- You get two datepickers: one from Chrome and one from jQuery. Chrome renders its activation button for the datepicker into the input field, and jQuery renders an additional button right after the input field. So at least the user can choose which one they'd like to use. Of course they'll be irritated.
- For some reason, Chrome renders a placeholder text into the text input field, no matter if I sent a valid String as value from the server side (a Smalltalk/Seaside Application in my case), and only if you select a date in one of the two datepickers, you see an actual date in the input field
It is things like this that makes it a hard requierement to test you web application in all browsers that you want to support. And by "all" I really mean all-damnit-%&$%&$ing-versions-of-all-kinds-of-browsers-you-want-your-application-to-look-and-feel-good-on.
So what do we learn from that?
- Be prepared to find tricky little problems with Ajax / jQuery on some version of some browser that don't show up anywhere else. You may have tested this stinkin page on IE, FF and Chrome, but be sure that the one Browser you forgot to also test it on can break the whole thing. Sometimes, it is something you may have seen, but never paid attention to, but it will come back as a trouble ticket.
- Be prepared to accept a looong test period in which you constantly hunt for impossible bugs that can only be reproduced in Version 19.5.105 of Browser X.
- Be prepared to either make compromises or spend double the estimated time on seemingly irrelevant problems that do not really affect your functionality, but look ugly or are possibly irritating for your users.
- Always resist equating JavaScript with portability or cross-platformness. You'll be punished for it, believe me.
- Forget about the 21st century. We're back in the mid-80ies.
(Sorry for this post, but I am a bit frustrated today)


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Palestine- What it's All About
2 Minute Video
As awareness across the world continues to increase regarding the Israeli Apartheid regime in Palestine, each effort in this aspect would help accelerate the conclusion of this shameful page in history.
The Story of Palestine
One might say that the victor is the one that writes history. The victor in this case will inevitably be justice, and justice is at the core of the Palestinian struggle against apartheid, colonialism and oppression.
Visit The Never Before Campaign
Filed under: Discrimination, Occupation Terrorism, Palestine | Tagged: Nahida |


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The Charter Township of Orion Zoning Board of Appeals will hold a public hearing on Monday, June 25, 2018 at 7:00 pm in the lower level of the Orion Township Hall, 2525 Joslyn Road, Lake Orion, Michigan 48360, on the following matter:
AB-2018-20, Baldwin Holdings, LLC, 4713 S. Baldwin Rd., 09-32-376-001
Two variances from Sign Ordinance 153, Section 7 Ground & Wall Signs In Non-Residential Zoned Areas, Ground Sign BIZ District,
1. A 3.3 sq. ft. variance above the allowed 50 sq. ft sign area to build a 53.3 sq. ft. sign.
2. A 10' front yard setback variance from the required 30' front yard setback to build a 6' tall ground sign 20' from front yard setback.
You may send correspondence regarding this case to the Orion Township Hall, addressed to the Zoning Board of Appeals, or you may attend the public hearing to express your views and/or concerns.
A complete copy of the proposed ZBA application is on file in the Planning & Zoning Department and may be examined during normal business hours, 8:30 am to 4:30 pm, Monday through Friday, until the date of the public hearing.
Orion Township will provide necessary and reasonable auxiliary aids, and services for individuals with disabilities at the public hearing upon advance notice by writing or calling Penny S. Shults, Township Clerk, 2525 Joslyn Road, Lake Orion, Michigan 48360; (248) 391-0304, ext. 4001. Please contact the Clerk's office at least 72 hours in advance of the public hearing.
Loren Yaros, Chairman
Zoning Board of Appeals
Charter Township of Orion


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Souper Bowl Sunday!
The February Soup Sunday is always on the same day as the Super Bowl, so we will see you on this coming Sunday February 4, 2018. If you can bring a pot of your favorite soup, or fruit, or bread, or dessert, that would be awesome!! As always, clean-up help is greatly appreciated.
Questions? Please contact Barbara Baglien or Jan Robinson.


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We are headed out in the morning for a 3 day tour to Rockport, Texas. I plan to catch some MAJOR Redfish and probably a hangover, too, but am also taking the camera(s) with me to try to get some snaps of the migratory birds moving into the area with the cold front this weekend. I am taking digital AND film … not too sure about my skills with the film yet. Just got my developing chems in and will start developing negatives next week! Exciting!
Don't forget about the new package deals I have going on for seniors and teens. I will be updating with Holiday packages next week! We will start those sessions in October ~ Don't miss out! Families, kids, pets – digital or old school film (lovely look to a vintage style card – or even retro-mod!) everyone should have a custom Holiday card! Sets start as few as $25 cards with gift prints… more to come!
Here's Griffy; getting set up for fishing. He likes to fish on the front porch for "cars"… hehe.
PS: These are digital files – you can tell because I will always add a white border to all digital images. 🙂


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I have an old porcelin (sp?) sink from my old house. It is pretty shallow, maybe 3" deep, reminds me of a dry sink. I wouldn't mind using it in our new house (more than likely just as decor) but it has something stuck to the porcelin. We can get rust stains off of it but not this other stuff, I wish I could say what it was but I don't know. Does any one have any advice on cleaning it? Thanks
One house that I lived in had some stains in the tub that I couldn't get off with cleaners, someone told me to use a dryer sheet, one that's been unused, get it damp and scrub away...it actually worked on whatever stains were in my tub. Maybe give that a try?:confused:
You could also use white vinegar, baking soda, or lemon juice. All are natural cleaning products that workamazing well considering how many chemicals are out there. Good. luck. Process of elimination.
Baking Soda is going to be your best natural cleaning tool. As an alkali, it can soften hard water and remove hard water calcification spots. Because it's an abrasive, you can also use it to clean almost anything.
To get rid of rust stains, use a little lemon juice. Because it's mildly acidic, it will take out the rust, but not your finish.
Check out my article on how to clean with natural cleaning products: http://www.associatedcontent.com/article/615323/sparkling_solutions_how_to_clean_with.html
* Denture cleaner. Makes rust and other stains disappear in seconds.
* Bathroom eraser. I found this at Menards in the plumbing department, and it cut through some hardened, raised stains in a tub.
Here's a corrected link to the bathroom cleaner.
I will try baking soda first, but I think I may have already tried that and it didn't work. and then I'll try the stain eraser thing from Menards. I'm not sure it it is just hard water deposits or some kind of dried adhesive but we'll give them all a whirl. Thanks.


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Danielle Mackey, better known as Panser, is a successful youtuber under the handle TradeChat. She has a massive following of over 265,000 subscribers who tune in to watch her videos about various games, such as League of Legends and World of Warcraft. Danielle has partnered up with some of the biggest fan sites of these games, providing weekly recaps for both wowinsider.com and surrenderat20.net. She gains an average of 500+ new subscribers per day and 1,000,000+ views per month. Danielle also has very large followings on her personal vlog channel, (55,000 subscribers) facebook, (84,000 fans) and twitter (42,000 followers.)
Danielle is also known for her role as War Princess on Playstation Network's The Tester 2. In this competition reality show she placed fifth. She also participated as a contestant for TBS's reality competition show, King of the Nerds, in which she placed third. In polls conducted by TBS, Danielle ranked in as the most popular contestant. Danielle is also a cosplayer, known for her creative spins on popular characters, such as Pikachu.*
You can find Danielle all over the internet:
Youtube: http://www.youtube.com/
Vlog Channel: http://www.youtube.com/
Facebook: http://www.facebook.com/
Twitter: http://www.twitter.com/
King of the Nerds: http://www.tbs.com/
The Tester 2: http://us.playstation.com/
*All information is as of February of 2014


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Longford hurlers hammer Sligo in warming up for the league final
Eoin Donnellan scored 1-10 as the Longford hurlers warmed up for the National League Division 3B Final against Warwickshire at Rathoath on Saturday next with a most emphatic win over a poor Sligo side at Glennon Brothers Pearse Park.
With Longford's place already secured in the league title decider prior to this rearranged fixture, Ray Gavin decided to give a number of young players a chance to impress and they didn't let their manager down. Paul Leonard did well at corner back, wing-back Stephen Walsh got a point while Reuben Murray – younger brother of Karl – scored 1-1.
Gavin was delighted with the performances of those players: "We made changes; we have young lads training with us all year and they deserved to play a National Hurling League game. Chaps needed to be rewarded. The lads will learn from that. For the development of those lads for the future of Longford hurling, this has to be good for them."
The display on Saturday last certainly gives Gavin some options when he decides on his first fifteen for the final. "There is nothing nailed down yet for the starting team in the promotion battle against Warwickshire."
There was a minute's silence before the Sligo game for Tony Corcoran, father of players Paddy and Ciaran, who passed away recently. Longford dominated from start to finish and had opened up a six point lead by the 8th minute after John Mulhern got the first goal.
Reuben Murray scored the second goal in the 24th minute and in first half stoppage time Cathal Mullane got a third to leave Longford in a very commanding 3-10 to 0-5 lead at the break.
The home county didn't let up on the scoring in the second half with Eoin Donnellan getting a fourth goal in the 58th minute while Rory Brennan hit over a few consolation points for Sligo.
Longford substitute Jamie Rosney added two fantastic long range points while Karl Murray scored a wonderful point from a sideline cut as the rampant midlanders ran out winners by the landslide margin of 23 points.
But Ray Gavin isn't getting carried away by the result. "We cannot afford to be complacent against Warwickshire. Both myself and Paddy (Walsh) have been around the block and we lost more finals than we won. We will take nothing for granted but the lads have a belief there and know they are good enough."
Having won their three games in Division 3B and finishing top of the table, Longford could miss out on promotion if they fail to beat Warwickshire. That is the realisation for them.
Gavin said: "It is a realistic fear. We played Warwickshire here and we beat them by five points. We got a goal with the last puck of the game. Warwickshire will think they can win the final. Who knows what will happen on Saturday. It's championship hurling for Longford from here on in and we have to win every game if we can."
With Longford now competing in the Nicky Rackard Cup their manager knows how important it is for his side to be playing in a higher division.
"We have to get up into Division 3A to be really able to compete in the Nicky Rackard Cup. We should be in it but we cannot get carried away. We just have to take it one game at a time. I would be hopeful that we can win the league final on Saturday," concluded Gavin.
LONGFORD: Paddy Cullen; Gerard Moore, Benny Stakem, Paul Leonard; Karl Murray (0-2, 1 free), Conor Flanagan, Stephen Farrell (0-1); Kevin Mealiff (0-1), Paul Barden jnr; Cathal Mullane (1-1), Martin Coyle (0-1), Luke Kelly; Eoin Donnellan (1-10, 5 frees), John Mulhern (1-1), Reuben Murray (1-1).
Subs:- Kealan Cox for M Coyle (injured, 25 mins); Jamie Rosney (0-2) for L Kelly (injured, 32 mins); Eoghan Kavanagh for P Leonard (46 mins); Dermot Kiernan for E Donnellan (59 mins); Conor Gallagher for C Mullane (60 mins).
SLIGO: Sean Hayes; Ronan Redmond, Tom Brennan, Bernard Feeney; Ethan McGowan, Jamie Kenny, Niall McDermott; Aidan Morrison, G Cadden (0-1); Peadar Leonard, Gerard O'Kelly-Lynch (0-1, free), Gary Waldron (0-1); Rory Brennan (0-5, 3 frees), Keith Raymond (0-1, free), Gary Gaughan.
Subs:- Danny Keown for G Cadden (half-time); Joe Moran for B Feeney (65 mins); Tony O'Kelly for G O'Kelly-Lynch (65 mins).
Referee: Alan Nash (Derry).


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ORDERING for
Home Delivery
We offer fresh CERTIFIED ORGANIC food straight to your door.
Home delivery is available every Monday in The Gap and surrounding areas including suburbs of:
- Ashgrove,
- Red Hill
- Paddington
- Milton
- Kelvin Grove
- Keperra
Email us if your suburb is not on this list and we will let you know if we can deliver to you.
All orders are placed by email: firstname.lastname@example.com
For your Monday delivery be sure to get your order in by 8am on Sunday. This gives us time to collate your orders Sunday, collect Monday and to gather and pack it fresh Monday morning for delivery straight to your door. Late orders are welcome but you may miss out on some items.
Our Fruit and Vegetables available for delivery and in the shop changes regularly (and so do prices) depending on the season and the weather.
We up date our prices every Sunday, so click on the product list in the lop left cornor of this page to see what's available this week…
We offer a flexible ordering system to suit every household
* order just what you want
* order weekly, fortnightly, monthly or occasionally
* specify your upper $$ limit
* order a set price box.


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Finding a river cruise from Venice
Robert Moore, of Farnham, Surrey, writes
I understand there is a river cruise that goes from Venice. We have used Titan Travel for a number of cruises but it does not cover this area. Do you have any information on operators that do, and do they offer trips of comparable quality to Titan's?
Jane Archer, cruise expert, replies
Titan Travel (0800 988 5867; titantravel.co.uk) is introducing eight-day river cruises from Venice – but not until next year. They are on the River Countess, which is owned and operated by Uniworld, and has recently undergone refurbishment. The vessel spends time in Venice, and also cruises the Po to Polesella, with included excursions to Padua, Bologna, Verona and Ravenna. Cruises cost from £2,299 per person, including flights, transfers in Britain and overseas, excursions, and drinks with lunch and dinner.
The French company CroisiEurope (0033 388 764066; croisieurope.com) used to cruise the Po but says a new European regulation has reclassified a key area from a river zone to a Maritime Zone D, on which their river cruise vessel is not allowed. As a result, it now stays mostly in Venice – there is just a short cruise to Chioggia – with optional excursions to Padua, Verona, Ferrara and Bologna.
CroisiEurope is neither as luxurious nor as inclusive as Uniworld – about 60 per cent of its passengers are French. Titan said it had not been informed of the new restriction and would be operating the Po cruises as planned.
For something more intimate, European Waterways (gobarging.com) has six-night cruises from Venice to Mantua and vice versa on the 20-passenger La Bella Vista. Prices start at £2,390 per person, which excludes flights but includes all drinks. The company says it is not affected by the new regulation.
Ask the experts
Our Q&A service allows you can pick the brains of our experts at home and abroad. Email your query to firstname.lastname@example.com. We won't be able to answer them all, but we will do our best.
About Jane Archer
Jane spends much of the year at sea, uncovering the best and worst about cruise ships.