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March was filed with guest blogging goodness, which relieved me of the duty of actually posting on my own blog. Nice! Thanks to everyone who participated and gave me a month off. It was time well spent. I decided to use my first post back to update you folks on all that is happening in Tonia Brown land. (At least on the writing front.) Consider this your quarterly update. Since the beginning of the year there had been lots and lots of news, some good, some bad, but all interesting. Let us recap the first couple of months and see what we have on our hands. Hang on tight, this won't take long.
First off, let's get over the bad news. Around the beginning of the year I was released from the contracts for several books, all at the same time. I know, its kind of weird timing but what can you do? Any who, my peeps can no longer get a hold of The Blooming, Epiphany or White Elephant. I might seek new publishers for these, but then again I might just let them rest a while. Each book had its time and folks enjoyed them while they were available, and I'm happy with that.
Don't fret, fair readers. Lucky Stiff is still available! It's gotten great reviews and folks really seem to like Peter's tale. Have you read it yet? Go get it! Now!
On the good news front, in the last few months have seen me enjoying the heck outa myself on internet radio. Specifically, blogtalk radio. Even more specifically, Flashes in the Dark Radio. Super sexy seductress, author Lori Titus, hosts the show with all her delicious hosting wisdom, and I sit in as her court jester and sidekick. I have said it before and I'll say it again: Lori does all the work here, folks. I'm the equivalent of giving a monkey a firecracker and a box of crayons. Without Lori, there would be no show. She just prays I keep my filthy mouth shut long enough for the guest to at least be able to say hello.
I penned a few guest blog posts the last few weeks, which was super fun. If anyone wants me to take a stab at your blog, just ask. I can't promise it will be Pulitzer material, or that there won't be mention of various bodily functions.
I've sold about ten short stories since the beginning of the year. Some of them are going reach the market much slower than others, but I kind of prefer it when a publisher takes their time with stuff like that. I'm also editing an erotic anthology, which should see the market soon. Can't wait for that one to come out. Nice!
Then there's my new web serial. I know I've mention this once here before, and I go on and on and on and on about it on Facebook, and I'm going to talk about it again. Railroad! is a steampunk adventure set in the American old West. It features a train that lays its own tracks, a crazy professor, and a troubled gunman. We are up to chapter five right now, so I suggest you get all caught up before the train gets away from ya.
Speaking of good news, recently I got the bestest news a writer could get! About this time last year I penned a new novel, The Cold Beneath. It's got steampunk and zombies and gadgets and super science and it's going to knock all of your collected socks right off your tootsies. Once I was done, I sent out a handful of agent queries and much to my surprise one of them not only nibbled, he ran off with my bait! As a result, I'm now operating under the auspices of an agent. Nick is such a great guy. He really loves the novel and I can't wait to see how he is going to pitch the thing. I have been super duper careful not to act like a total and complete weirdo around him so I don't spook him off. (Though I think he might suspect I'm not your average kind of gal.)
Last but not least, let us jaw jack about my upcoming backyard writer's convention, Brownstock. It's gonna be off da hook! Think weekend campout, only in my backyard. Yes, my backyard. This isn't a 'come and sell your books' kind of convention. This is a 'come and hang out and have a good time' kind of convention. Writers from all over are coming out for wieners, and zombie shooting and a ghost hunt. Gonna rock. Good times, folks. Good times.
Well, that's my quarterly update. Hope all is going well with you guys.
Catch you later, taters,


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Digital Compositor and 3D Artist
Light Production
- Conceptualising the design, and create visual effects.
- Integrated generated images into live action footage.
- Created 2D graphic elements to implement into live action scenes
- Modelling environments and characters.
- Rigging characters for animation.
- Working on multiple projects concurrently.

subset: commoncrawl


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27 Wolcott Street, Everett, MA, 02149
Thousands of Americans suffer from episodes of acute or persistent neck pain each year as a result of injury, strain, overuse or aging. The neck is more clinically referred to as the "Cervical Spine." However, a pain in the neck should not be ignored and left undiagnosed and untreated. Problems in the cervical spine, the first seven bones (vertebrae) in the neck running from the base of the brain to just above the shoulder blades, require assessment and treatment to prevent further, more permanent, damage. Chiropractic manipulation of the C-spine is often the treatment of choice and can be highly effective in treating a variety of neck pain issues.
As for us Chiropractors, the first thing we do AFTER a careful case history and initial examination of basic vitals would be to MOTION PALPATE the region to ascertain what, if any, vertebral segments are out of place. The easiest way to do this is look for a lack of normal motion in the individual segments. Thus, utilizing Motion Palpation (usually aided by X-Rays or some other form of imaging), we decide which aspects of the cervical spine need correcting. See: Lakhani E, Nook B, Haas M, et. al., "Motion Palpation Used As a Postmanipulation Assessment Tool for Monitoring End-Feel Improvement: A Randomized Controlled Trial of Test Responsiveness," J Manip. & Physio. Thera., Vol. 32 (7), Sept. 2009.
"The cervical spine is where the spinal cord lives," said Volker K.H. Sonntag, MD, a member of the American Association of Neurological Surgeons Board of Directors and a neurosurgeon practicing in Arizona. "The spinal cord is the most delicate tissue in the entire body. Even minor damage to the spinal cord cannot be repaired. Unlike low back pain, in which waiting to seek treatment may prolong the pain but usually doesn't cause any further damage, untreated spinal cord compression can lead to irreversible damage."
Three Types of Neck Pain:
There are three general types of cervical neck pain. 1) Dull pain within the neck itself that may also include radiations of pain down into the shoulder(s) and arm that often involves a herniated disc. *See diagram. 2) More of a sharp pain that may include arm and hand numbness but is primarily a pathomechanical issue. 3) Cord Compression. I'll elaborate below:
1) A herniated (bulging) disc in the spine pinching a nerve root in the neck often causes this first type of neck pain. Discs are found between each vertebra, or bone, in the spinal column. They serve as "shock absorbers" within the spine and have a gel-like center that makes them flexible, allowing the spine to bend and move. Some have used the analogy of a "jelly doughnut" to describe the discs in the human spine. On the outside is tough, fibrocartiledge similar to what is found in the knee or shoulder but inside is a gelatinous core made of mucopolysacharides that occasionally bulge out. However, because the discs are soft they can also bulge and become misshapen. When this occurs, they can place pressure on the spinal cord or irritate one of the nerves leading from the spinal cord out to the arms and upper torso. If the bulge becomes severe, the disc may herniate and push into the spinal canal. The result can be weakness, tingling, clumsiness and numbness in the arm and hands. Bulging discs can be caused by injuries like whiplash, stress on the spine by overuse, or by arthritis/degeneration in the spine.
2) The second type of neck pain is often more of a pathomechanical issue which is to say, the vertebrae are out of place or not moving properly. Additionally, we often see muscular imbalance issues in the neck associated with pathomechanics. The pain can be of a sharp, stiff irritation that casuses local neck pain. In the case where the muscle imbalance leads to vertebral pathomechanics, then this can lead to shoulder and arm pain radiations. Consider this recent article that apparently ends the discussion as to whether or not there are pain receptors in the facet joints of the cervical spine (It is thought that these same receptors are located in the mid and low back as well). The facets are where the vertebrae interlock with each other. *Chen C, Lu Y, Kallakuri S, Patwardhan A, Cavanaugh JM, "Distribution of A-Delta and C fiber receptors in the Cervical Facet Joint capsule and their response to stretch," J Bone Joint Surgery 88: 1807-1816, 2006. The conclusion here is that the existence of receptors in the facet joint capsule indicates that the capsule has pain and proprioceptive sensory functions. NB: A-Delta and C fibers are the only known nerve fibers that actually conduct pain sensation back to the spinal cord. Proprioception is one's ability to ascertain one's place in time and space.
3) Cord compression, also known as myelopathy, often occurs in the cervical spine and sometimes isn't experienced as 'pain' by patients at all. It usually involves numbness or weakness in the arms or legs, difficulty walking, loss of pain or temperature sensation in the hands and arms, poor balance and stiffness in the neck. In this case, there is pressure directly on the spinal cord. Because this type of "pain" is often not felt in the neck itself, it is easily misdiagnosed. The spinal cord has laminations of so-called "tracks" that run up and down and bring sensory signals to the brain and relay motor signals to the rest of the body from the brain. The outermost laminations involve sensory tracks for the low back and legs. Thus, if the spinal cord is compressed enough in the cervical spine, the patient will often "feel" distress in the LOW BACK or LEGS. In this scenario, the problem is NOT in the low back or legs but in the cervical spine and is a neurological event; the brain thinks the problem is lower in the body than it really is. Manipulation of the spine as well as traction are two conservative methods that often relieve cord compression in the cervical spine.
"We usually see patients with neck pain in different scenarios," Dr. Sonntag said. "One, they've been in some sort of accident and have suffered a whiplash-type injury. Two, they have a chronic injury caused by overuse, most likely caused by working at a computer for endless hours. Or they've experienced one of the first two scenarios in the past and now have arthritis or a tissue degeneration problem."
MRI of the cervical spine with spinal cord compression
Neck Injuries:
Neck injuries, which commonly occur in automobile or sports accidents, are not to be taken lightly. These types of injuries tradtionally fall under the catagory of "Soft Tissue Injuries" because the entire injury is with muscles, ligaments and other soft tissues. On the other hand, in the rare event of a broken bone in the neck, a neurosurgical consult is indicated. "If a vertebra in the neck is broken and left untreated, injury to the spinal cord can happen hours or days after the injury," explained Edward C. Benzel, MD, a spine surgery professor and practicing neurosurgeon in Ohio. "Because of the severe consequences and irreversible nature of spinal cord injuries, extreme caution needs to be taken with anyone who has suffered a direct injury to the neck." If the spinal cord is injured or bruised the patient can be paralyzed below the site of the injury. If a vertebra in the neck is broken or cracked, neurosurgeons often will stabilize the spine by fusion and instrumentation to prevent further injury to the spinal cord. Most people thankfully don't experience this kind of injury. The majority of sufferers experience what are commonly called "soft tissue injuries." See below.
A more common neck injury is whiplash. Chiropractic care excells at treating "whiplash" injuries as well as most of the simple strain/sprain type of neck pain issues that arise in daily living. Symptoms include neck stiffness, shoulder or arm pain, headache, facial pain and dizziness. Prompt chiropractic care utilizing some physical therapeutics but mostly manipulation of the spine is the most effective treatment for whiplash injuries even if there is a herniation of a disc in the cervical spine. If the symptoms still persist after chiropractic treatment four to six weeks later, or if there is severe weakness in the arms, hands or legs and conservative chiropractic treatment has not resolved the problem, a neurosurgical consult should be considered. *See our comprehensive page on Auto Accidents.
Degenerative Conditions :
The neck is also susceptible to osteoarthritis and degenerative disc disease, which can be caused by general wear and tear on the spine. The discs begin to lose their flexibility and ability to absorb stresses in the spine. Or, bone spurs develop on the vertebrae. In either case, the nerves in the cervical spine can become irritated or pinched, causing pain in the neck or the arms. If there is a great deal of degeneration in the cervical spine, the spinal cord and nerve roots may become compressed, causing irreversible damage.
Cervical stenosis is another condition that may result from degeneration in the spine. It occurs when the spinal canal narrows and compresses the spinal cord. If the pain from stenosis is restricted to the neck, conservative treatment is prescribed. However, if it extends into the arms or legs neurosurgeons can remove part of the vertebrae and ligaments to allow more room for the spinal cord and nerves. If there is significant degeneration in the cervical spine, the neurosurgeon can fuse the vertebrae by grafting bone or using metal plates to provide additional support and stability in the spine. Stenosis is admittedly tough for chiropractors to work with and we have traditionally had equivocal results with this painful condition.
27 Wolcott Street
Everett, MA, 02149


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- Research
- Open Access
A Poisson mixture model to identify changes in RNA polymerase II binding quantity using high-throughput sequencing technology
- Weixing Feng†1, 2, 4,
- Yunlong Liu†1, 2, 3,
- Jiejun Wu5,
- Kenneth P Nephew5, 6, 7,
- Tim HM Huang8 and
- Lang Li1, 2, 7Email author
© Feng et al; licensee BioMed Central Ltd. 2008
- Published: 16 September 2008
We present a mixture model-based analysis for identifying differences in the distribution of RNA polymerase II (Pol II) in transcribed regions, measured using ChIP-seq (chromatin immunoprecipitation following massively parallel sequencing technology). The statistical model assumes that the number of Pol II-targeted sequences contained within each genomic region follows a Poisson distribution. A Poisson mixture model was then developed to distinguish Pol II binding changes in transcribed region using an empirical approach and an expectation-maximization (EM) algorithm developed for estimation and inference. In order to achieve a global maximum in the M-step, a particle swarm optimization (PSO) was implemented. We applied this model to Pol II binding data generated from hormone-dependent MCF7 breast cancer cells and antiestrogen-resistant MCF7 breast cancer cells before and after treatment with 17β-estradiol (E2). We determined that in the hormone-dependent cells, ~9.9% (2527) genes showed significant changes in Pol II binding after E2 treatment. However, only ~0.7% (172) genes displayed significant Pol II binding changes in E2-treated antiestrogen-resistant cells. These results show that a Poisson mixture model can be used to analyze ChIP-seq data.
- Particle Swarm Optimization
- MCF7 Cell
- MCF7 Cell Line
- Binding Quantity
- Poisson Mixture Model
Massively parallel sequencing is a high-throughput technology capable of sequencing hundreds of thousands of DNA fragments in a single experiment. Combined with antibody-based chromatin immunoprecipitation assay (or ChIP-seq assay), this technology has been demonstrated to be a comprehensive, quantitative and cost-effective approach for mapping protein-DNA interaction on a genome-wide scale [1]. One ChIP-seq run can generate more than 20 million sequence tags of up to 36 bps each, which can then be definitively mapped to the human genome.
Due to the large amount of data generated in high-throughput sequencing experiments, innovative computational and statistical approaches are required to identify biological signals from ChIP-seq data. To date, several approaches have been applied to identify genomic regions containing a high concentration of sequence hits, i.e., "ChIP-seq peaks" [1]. An underlying assumption of current approaches is that DNA-binding proteins, such as transcription factors, contain sequence-specific, DNA binding domains that target a cluster of cis-acting DNA elements sharing certain sequence features. While such algorithms can identify DNA binding sites for highly specific transcription factors, current approaches are not appropriate for identifying binding sites for the general transcriptional machinery, such as RNA polymerase II (Pol II), which typically does not display high sequence specificity. In addition, as Pol II activity likely extends beyond the promoter/transcription start site of active genes, algorithms for assessing long-range Pol II binding are needed. Therefore, in this study, we have proposed a mixture model-based analysis for identifying differences in Pol II distribution in combined 5'-end, open reading frame (ORF), and 3'-untranscribed regions of active genes. Our strategy is based on the underlying assumption that the number of Pol II-target sequences follows a Poisson distribution and can be used to identify differentially transcribed genes under different experimental conditions. Furthermore, our proposed methodology can be used for making statistical inferences in experiments for which replicates are not available.
To date statistical models for ChIP-seq data are very limited. Nevertheless, new algorithms for ChIP-seq can be developed using existing framework for identifying differentially expressed genes from microarray data. For example, Kerr et al. [2] and Wolfinger et al. [3] employed analysis of variance (ANOVA) models to conduct a hypothetical test for different expression levels of individual genes in multiple microarray experiments. Dudoit et al. [4] used a t- statistic to address the problem of multiple comparisons through permutation analysis. These approaches yielded only p-values representing the probability of having an observed expression difference of a given gene, if the status is assumed to be the same before and after a treatment. However, for Pol II binding data derived from ChIP-seq, these approaches cannot be used to estimate the status change. A more appropriate choice analyzing ChIP-seq Pol II binding data may be a Bayes or an empirical Bayes approach. In this regard, Efron et al. [5] proposed an empirical Bayes model for calculating the probability of a differentially expressed gene given the observed data. As the empirical distribution for their t-like statistic for each gene does not share variation information, it works well only in situations involving at least a few replicates. Newton et al. [6] proposed another empirical Bayes model for cDNA microarray experiments with only one replicate. According to their method, if a gene is differentially expressed under two conditions, its level of expression is independently generated from the same distribution [6]; otherwise, the level of expression is the same between experimental and control samples. In their work, the observational component follows a Gamma distribution with mean μ g , and μ g itself follows an inverse Gamma distribution (prior component). This hierarchical model is often referred to as the Gamma-Gamma model. Specifically, all genes share the same distribution for the within-gene sampling errors, a crucial feature in their method, as no replicates were available in their data example. Kendziorski et al. [7] further extended the Gamma-Gamma model to situations where replicates were available. In addition, they developed a log-normal model for the observational component and a normal model for the prior component. They demonstrated a comparable performance for a lognormal-normal model and a Gamma-Gamma model. A major advantage in the methods proposed by Newton et al. [6] and Kendziorski et al. [7] is that information sharing is a consequence of the empirical Bayes approach. The model pools the variation information across all genes, making it well suited for data sets containing only a few replicates (e.g., 2 replicates), and we have successfully utilized this model framework to test the correlation among genome wide gene expression, DNA methylation, and histone acetylation [8, 9].
In the current paper, we propose a different model, Poisson mixture model, within the same empirical Bayes framework for identifying gene targets with differential Pol II binding activities in breast cancer MCF7 cell line under various conditions. ChIP-seq data processing, normalization, and statistical methods are proposed in the method section; analysis of ChIP-seq data from breast cancer cell lines (MCF7 and its tamoxifen-resistant subline OHT-MCF7) before and after treatment with 17β-estradial (E2), are presented in the result section, with conclusions at the end of the paper.
As described by Fan et al [10], MCF7 human breast cancer cells (American Type Culture Collection, Manassas, VA) and MCF7-OTH cells were cultured and treated with E2 (10-8 mol/L) for three hours. Then, cells were cross-linked with 1% formaldehyde and chromatin immunoprecipitation was done as previously described [11]. The antibodies against Pol II were purchased from Santa Cruz Biotechnology (Santa Cruz, sc-899 X and sc-8005 X). After immunoprecipitation and purification, ChIP DNA sample was run in 12% PAGE and the 100–300 bp DNA fraction was excised and eluted from the gel slice. Then, Illumina library was constructed and sequenced with Illumina/Solexa Genome Analyzer.
A Poisson mixture model to identify transcripts with different Pol II binding quantity
where λ ij denotes the expected quantity of Pol II binding for gene i under condition j (j = 1, 2). For the i-th gene, if Pol II binding quantities within the gene transcript region are statistically different between the two biological conditions, y ij follows marginal distributions with different parameters λi 1and λi 2; conversely, if the number of Pol II binding does not demonstrate a significant difference, y ij follows the same distribution with a unified λ. A mixture model is proposed to estimate the posterior probability of differential Pol II binding quantity.
Based on equation (4), we implement E-M algorithm by treating Z i as missing data.
In the M-step, the optimization procedure is challenging, because searching for the optimal solutions for Gamma parameters can be trapped into local optimum, causing either slow convergence or failure to converge on the global optimal solution. In order to overcome these difficulties, we utilize particle swarm optimization (PSO), an artificial intelligence approach that mimics a behavior of swarm-forming agents, providing a good balance between global optimum searching and computation efficiency [12].
Because the likelihood functions of (α1, β1) and (α2, β2) are factorized in equation 6, the PSO optimization procedures are conducted independently using the following four steps.
Step 1: 100 particles (potential solution) were initially randomly distributed in 2-dimensional parameter spaces (α1, β1) or (α2, β2).
Step 2: the likelihood of each of the 100 particles are calculated by following Equation 6.
where P global is the global optimal solution achieved so far; Pk-localis the local optimal solution achieved by particle k; and C0, C1, C2 are adjustable weight factors used to control searching speed.
Steps 1–4 will be iterated until further particle movement cannot result in higher likelihood (defined in Equation 6).
At the convergence, Z i can be interpreted as the probability of differential Pol II binding between two conditions. Although the model derivation is based on ChIP-seq data from MCF7 cells before and after treatment with vehicle or E2 for 3 hours, it can also be equally applied to OHT-MCF7 (+/- E2 treatment). In practice, the solution of the E-M algorithm converges in only 5 to 6 cycles.
Results and discussion
Genome-wide identification of Pol II binding in breast cancer cell lines
We tested our model on the Pol II binding quantity in MCF7 and OHT-MCF7 breast cancer cell lines (+/- E2 treatment) derived from the use of ChIP-seq technology. Among all the DNA fragments detected in each sample, we selected only those with high sequencing and matching quality that could be mapped to unique genomic locus. This pre-filtering step sufficiently removed background detection noise, and 2.59, 2.52, 3.00, and 1.33 million reads passed the above filter in MCF7 control, MCF7 E2-treated, OHT-MCF7 control, and OHT-MCF7 E2-treated samples, respectively. In order to compare Pol II binding quantity within a specific genomic region across multiple samples, the number of detected Pol II fragments was normalized using the total number of matched fragments in each sample, based on the assumption that the total number of DNA-binding Pol II would be similar in different cell types under different biological conditions.
Estrogen-induced changes in Pol II binding quantity in two cell types
The mean value and standard deviation of Pol II quantity in gene transcript regions in MCF7 and OHT-resistant MCF7 cells before and after E2 treatment.
Wild Type
Mean Value
Standard Deviation
Genome Pol II quantity changing level analysis
The gene numbers and percentage of whole genome in wild type and OHT-resistant MCF7 cells with minor change (0.1 ≤ Z < 0.9) or major change (Z ≥ 0.9)Pol II quantity.
Wilde type MCF7 Cell Genes
OHT-resistant MCF7 cell Genes
Minor change (0.1 ≤ Z < 0.9)
Major change (Z ≥ 0.9)
We report a Poisson mixture model to identify estrogen-induced changes in Pol II binding quantity in wild type MCF7 cells and OHT-resistant MCF7 cells. Despite having only one replicate available, our model successfully identified genes with different Pol II binding quantities from data derived using ChIP-seq technology. This model can distinguish differentially expressed Pol II activities from unchanged Pol II activities using a posterior probability calculated through an empirical Bayes approach. The empirical Bayes approach utilizes a combination of E-M and PSO algorithms for estimation and optimization in detection of the differential Pol II binding in two biological conditions. In this model, small signals require large changes in binding quantity to reach the same level of significance. The proposed model is unique in its ability to handle the ChIP-seq data without replicates and thus is an excellent tool for laboratories to evaluate preliminary ChIP-seq results. However, it is important to point out that despite the fact that no replicates are required to calculate changing probability, the use of biological replicates to capture persistent measurements in response to certain treatments are strongly encouraged.
This work is supported by National Cancer Institute grants CA085289 (KPN) and CA113001 (TH-MH), CSC (China Scholarship Council) Scholarship Programs (WF), and the Indiana Genomics Initiative of Indiana University (supported in part by the Lilly Endowment, Inc., YL).
This article has been published as part of BMC Genomics Volume 9 Supplement 2, 2008: IEEE 7th International Conference on Bioinformatics and Bioengineering at Harvard Medical School. The full contents of the supplement are available online at http://www.biomedcentral.com/1471-2164/9?issue=S2
Authors' Affiliations
- Johnson DS, Mortazavi A, Myers RM, Wold B: Genome-Wide Mapping of in Vivo Protein-DNA Interactions. Science. 2007, 316: 1497-10.1126/science.1141319.PubMedView ArticleGoogle Scholar
- Kerr MK, Martin M, Churchill GA: Analysis of Variance for Gene Expression Microarray Data. Journal of Computational Biology. 2000, 7: 819-837. 10.1089/10665270050514954.PubMedView ArticleGoogle Scholar
- Wolfinger RD, Gibson G, Wolfinger ED, Bennett L, Hamadeh H, Bushel P, Afshari C, Paules RS: Assessing Gene Significance from cDNA Microarray Expression Data via Mixed Models. Journal of Computational Biology. 2001, 8: 625-637. 10.1089/106652701753307520.PubMedView ArticleGoogle Scholar
- Dudoit S, Yang YH, Callow MJ, Speed TP: Statistical methods for identifying differentially expressed genes in replicated cDNA microarray experiments. Statistica Sinica. 2002, 12: 111-139.Google Scholar
- Efron B, Tibshirani R, Storey JD, Tusher V: Empirical Bayes Analysis of a Microarray Experiment. Journal of the American Statistical Association. 2001, 96: 1151-1161. 10.1198/016214501753382129.View ArticleGoogle Scholar
- Newton MA, Kendziorski CM, Richmond CS, Blattner FR, Tsui KW: On Differential Variability of Expression Ratios: Improving Statistical Inference about Gene Expression Changes from Microarray Data. Journal of Computational Biology. 2001, 8: 37-52. 10.1089/106652701300099074.PubMedView ArticleGoogle Scholar
- Kendziorski C, Newton M, Lan H, Gould M: On parametric empirical bayes methods for comparing multiple groups using replicated gene expression profiles. Presented at the Twenty-second Annual Conference of the International Society for Clinical Biostatistics. 19: 23-Google Scholar
- Khalili A, Potter D, Yan P, Li L, Gray J, Huang T, Lin S: Gamma-Normal-Gamma Mixture Model for Detecting Differentially Methylated Loci in Three Breast Cancer Cell Lines. Cancer Informatics. 2007, 2: 43-54.Google Scholar
- Li L, Shi H, Yiannoutsos C, Huang THM, Nephew KP: Epigenetic Hypothesis Tests for Methylation and Acetylation in a Triple Microarray System. Journal of Computational Biology. 2005, 12: 370-390. 10.1089/cmb.2005.12.370.PubMedView ArticleGoogle Scholar
- Fan M, Yan PS, Hartman-Frey C, Chen L, Paik H, Oyer SL, Salisbury JD, Cheng AS, Li L, Abbosh PH: Diverse gene expression and DNA methylation profiles correlate with differential adaptation of breast cancer cells to the antiestrogens tamoxifen and fulvestrant. Cancer Res. 2006, 66: 11954-11966. 10.1158/0008-5472.CAN-06-1666.PubMedView ArticleGoogle Scholar
- Lee TI, Johnstone SE, Young RA: Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc. 2006, 1 (2): 729-748. 10.1038/nprot.2006.98.PubMedPubMed CentralView ArticleGoogle Scholar
- Parsopoulos KE, Vrahatis MN: Recent approaches to global optimization problems through Particle Swarm Optimization. Natural Computing. 2002, 1: 235-306. 10.1023/A:1016568309421.View ArticleGoogle Scholar
- Fan MYPS, Hartman-Frey C, Chen L, Paik H, Abbosh P, Cheng ASL, Li L, Huang HMT, Nephew K: Breast cancer cells with acquired resistance to 4-hydroxytamoxifen and fulvestrant display divergent gene expression and DNA methylation signatures. Cancer Research. 2006, 66: 11964-11966.Google Scholar
- Carroll JS, Meyer CA, Song J, Li W, Geistlinger TR, Eeckhoute J, Brodsky AS, Keeton EK, Fertuck KC, Hall GF: Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006, 38: 1289-1297. 10.1038/ng1901.PubMedView ArticleGoogle Scholar
- Li L, Cheng ASL, Jin VX, Paik HH, Fan M, Li X, Zhang W, Robarge J, Balch C, Davuluri RV: A mixture model-based discriminate analysis for identifying ordered 18 transcription factor binding site pairs in gene promoters directly regulated by estrogen receptor-{alpha}. Bioinformatics. 2006, 22: 2210-10.1093/bioinformatics/btl329.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Using just some basic tools and a few pieces of glass, you can make these tile glass art pieces in a matter of hours.
This is a great way to start out and try some of the various embellishments.
Tiles make a perfect beginner project.
Take a blank piece of glass and add a few pieces of frit, stringers, or even some paint.
This will help you find out how these items fuse inside the kiln, how the colors change and what items you might want to try on other projects.
Materials Needed:
1. First measure and cut out one piece of the glass for the top of your tile and a piece for your tile bottom. You might want to use a piece of clear glass for the top of your piece. Use your glass cutter, cutter oil, and running pliers to cut and break the glass.
2. Use stringers, glass scraps, or glass paint to decorate your project. You can measure and cut a variety of sizes and shapes to make a design on your glass. You can try using frit or confetti on the surface for a one of a kind effect.
3. Clean all the glass pieces with glass cleaner or dish soap and hot water. Dry your pieces with a lint-free cotton towel. Now place your design on top of your bottom piece in your desired design, and top it off with the clear piece of glass. Be aware of any places that air might get caught and cause unwanted air bubbles. Using your finger tips, place the pieces on the prepared kiln shelf.
4. Fire the piece to a full fuse temperature of about 1450 F degrees and anneal your project. Let the temperature increase slowly, at a rate of about 600 degrees Fahrenheit per hour. The glass fusing process will take about 6 hours and roughly 6 more hours to anneal. The recipe to a long lasting fused glass piece is good annealing.
5. You can remove the tile from the kiln when the kiln has reached room temperature.
Fused glass tiles are not recommended for floors or counter tops as glass is extremely slippery when wet.
They can be used for as a back splash tile, decorative panels, or inlayed into wood.
These can also be turned into glass coasters.
The idea of a coaster is to keep the moisture from your glass from leaking all over the place.
If a flat tile is used, it will leak and drip onto your table.
It will also stick to the glass when you try to pick it up.
Flat tiles don't make good coasters.
To make a coaster, try cutting a circle out of 4mm fiber paper. Slump your square or round glass over this piece.
The fiber paper should give you a textured indentation that will help to hold the moisture from your glass.
Use a thin fire shelf paper on your shelf to add a smoother texture to the rim of your coaster.
Try adding some thin cork into this indentation to absorb more of the moisture.
Glue cork to the bottom of the coaster to keep it from scratching any furniture.
The possibilities of how to use these tile glass art pieces are endless.
Try different sizes and shapes to enhance your pieces.
Add some dichroic glass to your pieces for some unique dichroic glass tiles.
Return from this page to one of the following pages:
Glass Art for Beginners
Fusing Projects
Fused Glass Tile to Glass Fusing Made Easy


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Matchnight: United-Wizards
Greetings from Estadio RFK.
It wouldn't be a DCU home match without the threat of severe weather, now would it? Everything was fine on my drive to the Duomo on the Anacostia, but upon arrival, the rain began to fall and interrupt the Latin Festival in Lot 8. There is a permanent cloud over RFK, literally and figuratively.
Update: At 5:30, there is simultaneous bright sunshine and heavy rain.
Update: At 6:30, it's gorgeous. Go figure.
DCU: Wells; Namoff, McTavish, P. Carroll, Burch; Fred, Simms, Vide, Guerrero; Moreno, Emilio. Subs: Thorpe, Thompson, Zaher, Kirk, Murphy, Cordeiro, Dyachenko.
TV Notes: Bruce Arena is in the house working the KC broadcast booth. Thomas Rongen had travel delays returning from Europe, which means Dave Johnson will be on his own for much, if not all, of the local Comcast SportsNet coverage.
Don't forget to drop by the RFK training grounds tomorrow morning at 10 a.m. for the reserve match between the clubs.
As usual, this is the gathering point for pregame, in-game and postgame banter.
Steve Goff
August 2, 2008; 5:11 PM ET
Categories: D.C. United
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If You Don't Like The Weather, Change It, 2007
In the future, one of the great challenges for science will be to slow down the acceleration of aggressive weather phenomena. As the atmosphere heats up due to global warming, the difference in temperature between air masses will create more hurricanes, storms, and tornados. Also, if preventive measures to reduce CO2 emissions fail we might find ourselves in a situation where we have to take action not only to protect ourselves against the weather but also to actually modify and tame it. "If You Don't Like The Weather, Change It" is a film about the weather and how humans are trying to control the uncontrollable. We follow the trail from ancient rituals up to modern scientific methods to alter the climate. The film is presented as a quadroscopic video installation projected on parabolic discs. In using the round image and often a fish-eye view, the formal aspect of the presentation reinforces the concept of presenting an encompassing vision of a diverse topic where the earth's spherical atmosphere is at the end of the telescope.
Central to the film is our documented attempt to create a hole in a cloud. Using the technique of seeding clouds with dry ice, developed in the 1960s by Vincent Schaefer and Irving Langmuir, the performance executed above the clouds echoes of megalomaniacal experimentalism. The creation of a hole, or the production of "nothing", by releasing concentrated CO2 straight out into the atmosphere, could be paralleled to putting out a fire with gasoline. However, the dry ice is also a huge eraser with which we create an ephemeral work of art on the sky canvas. Adding to the timeline of stationary large-scale land-art projects, "If You Don't Like The Weather, Change It" takes the tradition of altering the landscape and inverts it into an immaterial sky-art project, slowly dissolving into thin air.
An exhibition catalogue produced in connection with the projects initial presentation at Uppsala Art Museum and Galerie Barbara Thumm in Berlin, can be downloaded as a pdf here:


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Product Description
Founded in 1961, Yves Saint Laurent was the first couture house to introduce the concept of luxury prêt-à-porter with the 1966 'Rive Gauche' collection, synonymous with youth and freedom. The brand continues to push the boundaries with edgy detailing, slick silhouettes and a coveted line-up of accessories.
Saint Laurent tan boots
Block heel measures approximately 70mm/ 3 inches
Tan suede
Beige leather lining, round toe
Concealed zip fastening along side
Made in Italy


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Know more about this business than we do? Cool! Please submit any corrections or missing details you may have.Help us make it right
Be the first OregonLive.com user to add photos or videos of Pacific Seafood & Restaurant
You'll be eating next to viewing windows of the oyster shucking and canning operation, so maybe not for the squeamish. The processing viewing is very interesting, though, and it provides a good???
The day I was there, they had an all-you-can-eat oyster & chips special, which turned out the be amazing. Later in the week was all you can eat cod fish & chips, but I missed that.The oysters were???


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3 Tips For Practicing The Art Of Generosity
Yoga has changed my view of generosity. I've learned to give without expectation and to always remain mindful of the quality of my generosity. After all, generosity in yoga is based partly on two of the five Yamas:
1. Generosity is the opposite of taking (Asteya), whereby we share freely with a focused, quality effort.
2. A generous person sees life through a prism of abundance instead of scarcity. In yoga this is known as Aparigraha: there is enough for everyone.
One of my favorite stories about the value of generosity is called Stone Soup. Each variation of the story is slightly different, and the author is unknown. Each involves a traveler who arrives in a town beset by famine. The inhabitants — fearing he'll want free food — try to discourage him from staying. "There is no food in this entire town!" they tell him. The traveler explains that he doesn't need any food and that, in fact, he was planning to make a soup to share with them. The villagers watch suspiciously as he builds a fire and fills an enormous pot with water. With great ceremony, he pulls a stone from his bag and drops it into the pot of water. He sniffs the brew extravagantly and exclaims how delicious stone soup is. As the villagers begin to show interest, he mentions how the soup might improve with just a little cabbage in it. A villager brings out a cabbage to share. This episode repeats itself until the soup has cabbage, carrots, onions, and beets, indeed, a substantial soup that feeds everyone in the village.
This story teaches us that sharing is especially important when we perceive a limitation, but it can also apply to sharing energy, putting conditions on giving love, or holding back our ideas (perhaps because we are afraid that others will "copy" us). The traveler represents the potential within each of us to inspire others to be more generous, and in the spirit of that traveler, below are three ways you can add more generosity to your life.
1. Remember that your most valuable gift is time.
Begin by being generous with yourself. Can you give yourself the gift of a yoga class every week so you feel the goodness of your own heart? Then share your time by calling someone to listen, offer to walk a friend's dog, babysit, go grocery shopping for a friend, volunteer at a soup kitchen.
2. Consider the quality of your generosity.
It's one thing to give away things you don't like or are bored with, but what about something more important? It's easy to get hung up on the idea of having or doing MORE for the sake of more. However, the philosophy that more is better forsakes quality. Go beyond this by pulling a couple things from your closet that you love, and give them away.
3. Don't hold back your ideas.
For years I've shared class plans, yoga playlists, philosophical class themes, and posted detailed yoga retreat itineraries online, all with the intention that they'll inspire others. I consult for free to help others cultivate new business ideas. I want students to build on what I do to create something even better. To me, imitation is flattering. It's our responsibility as members of a human tribe to be as generous as possible in sharing our intellectual currency.
Philosopher Maimonides pictured giving on eight spiritual levels. The first two get to the heart of yoga right away.
"The motivation for real giving finds its source in the internal self, not in the expectations of others."
"Happy people don't expect a return. They give because it comes from the heart and they believe that joy and happiness are abundant. They aren't going to run out."
Let's come together and open our hearts to all the ways we can be more generous with our spirit, our positive energy, our kind thoughts, our love, our time. And remember your personal understanding of the value of sharing is a reflection of who you are. Love yourself, love your day, love your life.
Ready to learn more about how to unlock the power of food to heal your body, prevent disease & achieve optimal health? Register now for our FREE Functional Nutrition Webinar with Kelly LeVeque.


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Use miles to save at the pump
You can also use miles for savings on BP fuel. On each transaction, you can choose to use 60 miles per gallon to save $0.50 per gallon4 by swiping your linked credit or debit card at the pump. As an added perk, you'll still earn miles for each gallon, even if you've used miles on the same purchase.


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Paper of the Month | 2006
January | February | March | April | May | June | July | September
January | top
Local environmental effects on the structure of the prion protein
DeMarco M.L. and Daggett V.
Comptes Rendus Biologies 328: 847-862, 2005
Prion diseases are neurodegenerative diseases causally linked to the partial unfolding and subsequent misfolding and aggregation of the prion protein (PrP). While most proteins fold into a single low energy state, PrP can fold into two distinct isoforms. In its innocuous state, denoted as PrPC, the protein has predominantly α-helical secondary structure, however, PrPC can misfold into an isoform rich in extended structure capable of forming toxic and infectious aggregates. While prion disease is believed to be a protein-only disease, one not requiring any non-protein elements for propagation, the different environments the protein finds itself in vivo likely influence its ability to misfold and aggregate. In this review we will examine various molecules, covalent modifications and environments PrP faces in vivo and the effect they have on PrP's local environment and, potentially, conformation. Included in this discussion are: (1) pH, (2) carbohydrates, (3) lipid membranes, (4) metal ions, and (5) small molecules.
February | top
Characterization of Two Distinct β2-Microglobulin Unfolding Intermediates that May Lead to Amyloid Fibrils of Different Morphology
Armen R.S. and Daggett V.
Biochemistry 44: 16098-16107, 2005
The self-assembly of β2-microglobulin into fibrils leads to dialysis-related amyloidosis. pH-mediated partial unfolding is required for the formation of the amyloidogenic intermediate that then self-assembles into amyloid fibrils. Two partially folded intermediates of β2-microglobulin have been identified experimentally and linked to the formation of fibrils of distinct morphology, yet it remains difficult to characterize these partially unfolded states at high resolution using experimental approaches. Consequently, we have performed molecular dynamics simulations at neutral and low pH to determine the structures of these partially unfolded amyloidogenic intermediates. In the low-pH simulations, we observed the formation of α-sheet structure, which was first proposed by Pauling and Corey. Multiple simulations were performed, and two distinct intermediate state ensembles were identified that may account for the different fibril morphologies. The predominant early unfolding intermediate was native-like in structure, in agreement with previous NMR studies. The late unfolding intermediate was significantly disordered, but it maintained an extended elongated structure, with hydrophobic clusters and residual α-extended chain strands in specific regions of the sequence that map to amyloidogenic peptides. We propose that the formation of α-sheet facilitates self-assembly into partially unfolded prefibrillar amyloidogenic intermediates.
March | top
The 108M Polymorph of Human Catechol O-Methyltransferase Is Prone to Deformation at Physiological Temperatures
Rutherford K., Bennion B.J., Parson W.W., and Daggett V.
Biochemistry 45: 2178-2188, 2006
The human gene for catechol O-methyltransferase (COMT) contains a common polymorphism that results in substitution of methionine (M) for valine (V) at residue 108 of the soluble form of the protein. While the two proteins have similar kinetic properties, 108M COMT loses activity more rapidly than 108V COMT at 37°C. The cosubstrate S-adenosylmethionine (SAM) stabilizes the activity of 108M COMT at 40°C. The 108M allele has been associated with increased risk for breast cancer, obsessive-compulsive disorder, and aggressive and highly antisocial manifestations of schizophrenia. In the current work, we have constructed homology models for both human COMT polymorphs and performed molecular dynamics simulations of these models at 25, 37, and 50°C to explore the structural consequences of the 108V/M polymorphism. The simulations indicated that replacing valine with the larger methionine residue led to greater solvent exposure of residue 108 and heightened packing interactions between M108 and helices α2, α4 (especially with R78), and α5. These altered packing interactions propagated subtle changes between the polymorphic site and the active site 16 Å away, leading to a loosening of the active site. At physiological temperature, 108M COMT sampled a larger distribution of conformations than 108V. 108M COMT was more prone to active-site distortion and had greater overall, and SAM binding site, solvent accessibility than 108V COMT at 37°C. Similar structural perturbations were observed in the 108V protein only at 50°C. Addition of SAM tightened up the cosubstrate pocket in both proteins and prevented the altered packing at the polymorphic site in 108M COMT.
April | top
Importance of Context in Protein Folding:
Secondary Structural Propensities versus Tertiary Contact-Assisted Secondary Structure Formation
Scott K.A., Alonso D.O.V., Ping Y., and Daggett V.
Biochemistry 45: 24153-4163, 2006
Molecular dynamics simulations can be used to reveal the detailed conformational behaviors of peptides and proteins. By comparing fragment and full-length protein simulations, we can investigate the role of each peptide segment in the folding process. Here, we take advantage of information regarding the helix formation process from our previous simulations of barnase and protein A as well as new simulations of four helical fragments from these proteins at three different temperatures, starting with both helical and extended structures. Segments with high helical propensity began the folding process by tethering the chain through side chain interactions involving either polar interactions, such as salt bridges, or hydrophobic staples. These tethers were frequently nonnative (i.e., not i → i + 4 spacing) and provided a scaffold for other residues, thereby limiting the conformational search. The helical structure then propagated on both sides of the tether. Segments with low stability and propensity formed later in the folding process and utilized contacts with other portions of the protein when folding. These helices formed via a tertiary contact-assisted mechanism, primarily via hydrophobic contacts between residues distant in sequence. Thus, segments with different helical propensities appear to play different roles during protein folding. Furthermore, the active role of nonlocal side chains in helix formation highlights why we must move beyond simple hierarchical models of protein folding.
May | top
The Folding Pathway of Spectrin R17 from Experiment and Simulation:
Using Experimentally Validated MD Simulations to Characterize States Hinted at by Experiment
Scott K.A., Randles L.G., Moran S.J., Daggett V., and Clark J.
Journal of Molecular Biology 359: 159-173, 2006
We present an experimental and computational analysis of the folding pathway of the 17th domain of chicken brain α-spectrin, R17. Wild-type R17 folds in a two-state manner and the chevron plot (plot of the logarithm of the observed rate constant against concentration of urea) shows essentially linear folding and unfolding arms. A number of mutant proteins, however, show a change in slope of the unfolding arm at high concentration of denaturant, hinting at complexity in the folding landscape. Through a combination of mutational studies and high temperature molecular dynamics simulations we show that the folding of R17 can be described by a model with two sequential transition states separated by an intermediate species. The rate limiting transition state for folding in water has been characterized both through experimental Φ-value analysis and by simulation. In contrast, a detailed analysis of the transition state predicted to dominate under highly denaturing conditions is only possible by simulation.
June | top
Protein Folding-Simulation
V. Daggett
Chemical Reviews 106: 1898-1916, 2006
July | top
Φ-Analysis at the Experimental Limits: Mechanism of β-Hairpin Formation
Petrovich M., Jonsson A.L., Ferguson N., Daggett V., and Fersht A.R.
Journal of Molecular Biology 360: 865-881, 2006
The 37-residue Formin-binding protein, FBP28, is a canonical three-stranded β-sheet WW domain. Because of its small size, it is so insensitive to chemical denaturation that it is barely possible to determine accurately a denaturation curve, as the transition spans 0-7 M guanidinium hydrochloride (GdmCl). It is also only marginally stable, with a free energy of denaturation of just 2.3 kcal/mol at 10°C so only small changes in energy upon mutation can be tolerated. But these properties and relaxation times for folding of 25 μs-400 μs conspire to allow the rapid acquisition of accurate and reproducible kinetic data for Φ-analysis using classical temperature-jump methods. The transition state for folding is highly polarized with some regions having Φ-values of 0 and others 1, as readily seen in chevron plots, with Φ-values of 0 having the refolding arms overlaying and those of 1 the unfolding arms superimposable. Good agreement is seen with transition state structures identified from independent molecular dynamics (MD) simulations at 60, 75, and 100°C, which allows us to explore further the details of the folding and unfolding pathway of FBP28. The first β-turn is near native-like in the transition state for folding (experimental) and unfolding (MD and experiment). The simulations show that there are transient contacts between the aromatic side-chains of the β-strands in the denatured state and that these interactions provide the driving force for folding of the first β-hairpin of this three-stranded sheet. Only after the backbone hydrogen bonds are formed between β1 and β2 does a hydrogen bond form to stabilize the intervening turn, or the first β-turn.
September | top
α-Sheet: The toxic conformer in amyloid diseases?
V. Daggett
Accounts of Chemical Research 39: 594-602, 2006
A novel secondary structure, the α-sheet, was identified through molecular dynamics (MD) simulations of various proteins associated with amyloid diseases under amyloidogenic conditions. The structure was first predicted by Pauling and Corey, and it has been directly observed in crystal structures of "nonnatural peptides." There are occurrences of α-strands and α-sheets in the Protein Data Bank, but they are rare. We propose that α-sheet is formed during the conformational changes associated with amyloidosis and that it may represent the toxic conformer. Here, structural properties of the α-sheet, background information, and experimental support for this novel structure are presented. Finally we speculate about the possible role of this conformation in disease.


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I hope you're all well and have had a lovely weekend. It's actually sunny today - yay!
So I don't know about you but one of the main things I look forward to most about spring is changing up my make-up routine! Of course, that means wearing a new foundation...
FACE: MAC Fix+, Estee Lauder Doublewear Concealer in Light, MAC Studio Finish Concealer in NW15, L'Oreal True Match Foundation in N1 Ivory (applied with ELF Stippling Brush), MAC Mineralize Skinfinish in Light, Benefit Hoola Bronzer (applied with ELF Studio Complexion Brush), MAC Fix+.
EYES: Urban Decay Primer Potion (Original), Sleek Storm Palette (taupe and matte brown shades), Soap & Glory Supercat felt tip eyeliner, Avon Color Trend Kajal Stick on lower waterline, CoverGirl LashBlast Mascara.
EYEBROWS: MAC Eyebrow Pencil in Dirty Blonde.
LIPS: The Body Shop Cocoa Butter lip balm.
Final thoughts:
I've been using L'Oreal True Match foundation for a couple of weeks now and I'm truly really impressed with it. The consistency is quite thin and runny but it's perfect for using as a base layer to even out your skin tone and mask imperfections.
Because the consistency is nice and creamy, it's really easy to add layers and make the coverage heavier if you require it - I certainly have been!
It doesn't go cakey or oxidise and you don't get the dreaded neck lines. The coverage is really natural looking and blends into the skin perfectly. I'm so glad I found it!
What's your foundation of choice at the moment? Have you tried L'Oreal Paris True Match? Let me know over on Twitter now or in the comments below!


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Dyson Dc17 Animal Bagless Upright VacuumReviewing: Dyson Dc17 Animal Bagless Upright Vacuum | Rating:
I have never in my life paid so much for a cleaning implement. And I have never in my life been so glad that I did!
A friend kept telling me how wonderful her Dyson is. It cleans better than any vacuum, it never clogs, on and on and on. I seriously began to worry about her as she raved about her beloved Dyson. I may get that excited about an upcoming vacation, an especially good report card that one of my kids brings home, but a vacuum cleaner? No way.
After fighting my old, difficult to use and unreliable vacuum one time too many I threw up my hands knowing deep down that I had to make a change. I drug myself, nearly kicking and screaming, to my local home improvement store, took a deep breath, gritted my teeth and hauled that baby right up to the register. I knew I was about to shell out more of my hard earned money than I really thought was sane.
Once I got it home and put it together and plugged it in, I waited for the big let down to come. I began the pushing and pulling, just knowing that I would find streaks of dust and dirt and pet hairs left behind like with my old vacuum. All I saw though was my carpet. My clean carpet. My carpet looking almost as good as when it was new. No pet hairs at all! How could this be? I pulled out the telescoping wand and crevice tool and hit those stringy webs up in the corners near the ceiling expecting them to fall on my head. Nope, they were gone. The stairs, my least favorite vacuuming chore, I just knew the Dyson would let me down there but with the stair tool, I was amazed at how easy they were also. Then I took a look at the dirt collection canister and was ashamed at the amount of dirt in the thing. Dirt from my house. How could that much dirt have been in my house?
All I can say is that I love my Dyson. Never again will I try to clean my house without a Dyson and never again will I complain about the price of it. Now I see the difference in a quality vacuum and an affordable vacuum. I cannot afford not to use Dyson.


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An Organized Research Unit (ORU) is an academic unit the University has established to provide a supportive infrastructure for interdisciplinary research complementary to the academic goals of departments of instruction and research. The functions of an ORU are to facilitate research and research collaborations; disseminate research results through research conferences, meetings, and other activities; strengthen graduate and undergraduate education by providing students with training opportunities and access to facilities; seek extramural research funds; and carry out university and public service programs related to the ORU's research expertise. An ORU may not offer formal courses for credit for students of the University or for the public unless it has been specifically empowered to do so by the President after consultation with the Academic Senate and the appropriate Chancellors.
To establish a Center within an Organized Research Unit (ORU), the faculty members concerned should submit a brief proposal stating the proposed unit's goals and objectives. The proposal should describe the value and capabilities that will be added by the new unit. It should make clear how the Center might foster new intellectual collaborations, stimulate new sources of funding, and/or further innovative and original research. The proposal should also contain the following information:
The proposal is first submitted to the ORU director for endorsement. The ORU director's endorsement implies the commitment of administrative support and space, if needed, to the unit. The director's statement of support for establishment of the Center should include a plan for timely review. The plan should contain benchmarks and metrics for the evaluative review that will result in a recommendation for continuation or disestablishment.
The proposal is then submitted to the Vice Chancellor for Research who seeks the counsel and advice of the appropriate Provost and/or Deans and department chairs, and divisional Academic Senate committees, usually the Council on Planning and Budget and the Council on Research and Instructional Resources. The Vice Chancellor for Research retains final authority in establishing the Center.
Each ORU Director is aided by a standing Advisory Committee, chaired by a faculty member other than the Director, which meets regularly and participates actively in setting the unit's goals and in critically evaluating its effectiveness on a continuing basis. Specifically, the Advisory Committee provides counsel to the Director on all matters pertaining to the unit, including budgetary matters and personnel. The charge to the committee also includes active participation in the planning and evaluation of the ORU's programs and activities. The Chair of the Advisory Committee, and as many other members as practical should meet with five-year review committees and otherwise be available for consultation by the five-year review committee during the course of its review. The Advisory Committee is made up predominantly of faculty members, but may include some members from the professional research series and may have some members from outside the University. The Advisory Committee is appointed by the Vice Chancellor for Research.
In accordance with the UCOP Policies and Procedures Concerning Organized Research Units, the Office of Research requests that each ORU prepare an annual report. See the Call for Annual Reports for the 2017-2018 Fiscal Year.
Annual Report and Supplementary Forms


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The Typical Boating Accident
Most boating fatalities don't have anything to do with bad weather or hazardous sea conditions. They typically occur in smaller, open boats on inland waters during daylight hours when weather and visibility are good, the winds are light, and the water is calm. Despite these ideal conditions, passengers fall overboard and many boats capsize, causing over half of all boating fatalities.
Capsizing, Swamping, or Falling Overboard
Capsizing is when a boat turns on its side or turns completely over. Swamping occurs when a boat stays upright and fills with water. Sometimes a person falling overboard from a boat causes the boat to capsize or swamp. Regardless, the outcome is the same—people are in the water unexpectedly.
Help Prevent Capsizing, Swamping, or Falling Overboard
To help prevent and prepare for capsizing, swamping, or someone falling overboard, follow these guidelines.
Tips for Small Craft Boaters
Small craft boaters need to be especially careful to avoid falling overboard. Falling overboard and drowning is the major cause of fatalities for small boats. To prevent falling overboard:
Bow Riding is Risky
Sitting on the gunwale, bow, seat backs, or any other area not designed for seating is risky behavior and can result in falling overboard. It is illegal in many states.


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Indian police say the "dangerous rooster" is thought to have killed Singrai
Soren after being forced back into the ring soon after his last fight.
The animal had emerged victorious, but witnesses said the victim died after
the feathered fiend cut his throat with razor blades attached to its legs as
he tried to immediately force it into another bout.
Villagers were warned not to approach the animal cops described as "an unknown
rooster with black and red feathers".
Police want to find the bird to strip it of the deadly blades he killed with,
but said with so many roosters matching his description the chances of
catching him were slim.
A friend of the victim, named only as Dasai, said: "The rooster tried to get
away from the ring several times but Soren tried to push him into the ring
"This upset him and he attacked."
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Author: DavidBryde
Cash Passport gives peace of mind for trips to Paris and Thailand
Our Product manager David Bryde says peace of mind and security were the key reasons he took a Cash Passport when he travelled to Thailand and France last year.
As much as The Greater takes as many security measures as it can to keep our money safe here and overseas, the main reason we initially used a Multi Currency Cash Passport was to keep our everyday account safe and separate from our travel money.
We could have used the debit card linked to our everyday savings account but any chance of a fraudster getting access to it while we were away was eliminated. We spent enough of our own money overseas without giving anyone else a remote chance of getting their hands on funds in our accounts down the track.
For our trip to Thailand we loaded the card with Aussie dollars but could have also loaded Thai Baht. It meant that the exchange rate varied from purchase to purchase but it was easy to keep an eye on the balance we had left on the card via the Cash Passport website.
We were able to use the card everywhere we tried, though we did learn pretty quickly not to make small ATM withdrawals in Thailand as the local ATM owners charge about $5 AUD per withdrawal, regardless of the type of card you have.
It was good that the couple of hundred dollars we had left on the card when we came home could be spent here at merchants via EFTPOS at no cost to us.
When the Paris trip came up it was easy to load the same card with Euro and we liked that there wasn't a new PIN number to remember. This time the exchange rate was locked in at the time we transferred money onto it.
No ATM fees in France made it easy to get cash whenever we needed it and there were no problems using it with merchants.
When we had a short stopover at the Bangkok Airport Hotel on the way home, it was quite clever how our bill was paid first from the remaining Australian dollars on the card and then from the Euro. It tidied the card up to only have one currency left on it.
With no travel plans (or budget!) on the horizon, it cost us $10 to close the card and convert the Euro back to Aussie dollars. That is a small price to pay for the peace of mind the card gave us in knowing we didn't have to risk the security of our everyday account in even the tiniest way.
The Greater can help you to get the right travel money option for your overseas holiday as well as your other necessary travelling companion – a travel insurance policy. Let the experts at The Greater take the stress out of that final bit of planning your holiday. Call into your nearest branch or phone 1300 651 400.
Have you travelled with a Cash Passport? Please tell us how you found it or share your travel money tips below.


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postcards of carcassonne
tags carcassonne, france, publication
3 commentaires:
- Barcelona apartments a dit…
Merci pour les photo's. Je retrouve un apartment dans Apartment Barcelona. C' est tres belle.
- 9/3/12 00:06
- Barcelona Apartments a dit…
Barcelona apartments are the preferred choice for a comfortable stay in Barcelona. Short Stay Barcelona lists many apartments in Barcelona that have been carefully selected and are managed by local management.
Comfortable Stay in Barcelona Apartments Barcelona
- 26/7/12 09:20
- Barcelona Apartments a dit…
Mixing up the old with the new, the Paseo del Borne Loft C packs an appropriately stylish punch since this holiday apartment is located in the city's most fashionable neighbourhood.
Fashionable Barcelona
- 31/7/12 08:43
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text: For the sauce: Whisk the eggs in a large bowl. Fry the bacon in a large frying pan over medium heat until crisp and golden, and then remove and drain on a paper towel. Leave all but 1 tablespoon of the bacon fat in the pan and add the butter. Return the pan to the burner and cook the onions until slightly golden, 2 to 3 minutes. Lower the heat to medium-low and add the half-and-half and cheese. Simmer until the sauce has thickened slightly, about 5 minutes; do not let boil. Let the sauce cool slightly, about 2 minutes. For the pasta: Boil the pasta in salted water until al dente. Slowly pour the cream sauce into the eggs while continuously whisking. Add the hot pasta and toss quickly. The hot pasta will complete the cooking of the eggs. Add the bacon and parsley and toss again. Season with salt and pepper and serve hot.


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North America
A northern subcontinent of the Americas!
North America (Spanish: América del Norte or Norteamérica; French: Amérique du Nord; Dutch: Noord-Amerika; Papiamento: Nort Amerika; Kalaallisut: Amerika Avannarleq) is the northern continent of the Americas, situated in the northern hemisphere and in the western hemisphere. It is bordered on the north by the Arctic Ocean, on the east by the North Atlantic Ocean, on the southeast by the Caribbean Sea, and on the west by the North Pacific Ocean; South America lies to the southeast.
The Americas are usually accepted as having been named after the Italian explorer Amerigo Vespucci by the German cartographers Martin Waldseemüller and Matthias Ringmann.
The prevalent languages in North America are English, Spanish, and French. The term Anglo-America is used to refer to the anglophone countries of the Americas: namely Canada (where English and French are co-official) and the United States.
Places and informational links for North America:
The term North America has many definitions on location and context. In English, North America may be used to refer to the United States and Canada together. Other usage sometimes includes Greenland and Mexico (as in the North American Free Trade Agreement), as well as offshore islands.
In Ibero-America (the countries in the Americas that were formerly colonies of Spain or Portugal) and other parts of Europe, North America usually designates a subcontinent of the Americas containing Canada, the United States, and Mexico, and often Greenland, Saint Pierre et Miquelon, and Bermuda.
North America has been historically referred to by other names. Spanish North America (New Spain) was often referred to as Northern America, and this was the first official name given to Mexico.


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To contribute to this site, see above menu item "About".
These transcriptions may contain human errors.
As always, confirm these, as you would any other source material.
Will of Alice Nowlan
In re
This is the last will of me Alice Nowlan of St. John's Spinster. First I give devise and bequeath to the Revd. Richard Howley of St. John's Doctor of Divinity the sum of twenty pounds currency to be disposed of my him in Masses or otherwise towards the repose of my soul in such way as to him shall seem proper. Second- I give devise and bequeath to my sister Judy Thomas wife of James Thomas Farmer near Charlottetown in Prince Edward Island the sum of twenty five pounds currency to be for her own proper and separate use behoof and benefit free and independent of all debts claims and liabilities of her present or any future husband. Thirdly, I give devise and bequeath to my other sister Mary Cooney of Nine Mile House in the County of Tipperary Ireland Widow the sum of twenty five pounds currency. Fourthly. I nominate and appoint the said Reverend Richard Howley as executor of this my last will and testament giving and granting unto my said executor full power and authority to liquidate and discharge all my debts funeral expenses cost of grave and all other expenses attending upon the carrying into effect the trusts contained in this my last will and testament. As witness my hand this fifth day of February Anno Domini one thousand eight hundred and sixty four. Alice her X mark Nowlan (LS) Signed sealed published and declared by the said testatrix as and for her last will and testament in presence of us who in her presence and in presence of each other have hereunto set our hands as witnesses having been first read over and explained to the said testatrix, Richard Howley. Robt. J. Kent.
Certified correct,
Note: The wills in those will books are NOT actual wills. They are either hand-written copies or in later years typed copies of a, "last will and testament," written or typed by the court clerk, after the death of the testator, when the executor presented them to the court for probate. The court clerk didn't list the signatures at the bottom, he (or she) just put them in the book in whatever order they were in, on the original document, no spacing most of the time, no punctuation and also no paragraphs. The originals were kept by the executor.
We who have typed these wills, have made every effort to include all the errors that were on the microfilm, in order to avoid destroying the integrity of the originals, where ever they may be. However, in some of the very long wills, we have tried to insert paragraphs to make it easier for the researcher to read the document.
Page Contributed by Judy Benson & Ivy F. Benoit
Page Revised by Ivy F. Benoit (Wednesday February 20, 2013)
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Welcome to Masterpage Indonesia
Established in 2006, MasterPage is the expert on web design and web development. Since then, we have been doing many web projects. Nowadays, the development of technology has change the need for website from just a company profile into a lifestyle. Whereas the internet has been very close and can be accessed easily from your hand.
That's why we are using the Responsive Web Design Technology to ensure your website to be displayed perfectly on any devices, not only on the desktop computers but also on the tablets and mobile phones. You may enjoy the optimal viewing experience using the Easy Reading and Navitation System.


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Full featured image management. Fully integrated with HPE TRIM, Records Manager and Content Manager.
Latest version: 3.4.6394, released 24 July 2017.
Exisiting Use Cases:
Improve Productivity
Sigma Pictures enables your workforce to quickly import pictures into HPE CM / HPE RM / HP TRIM (HPE CM) from cameras, mobile phones and file directories. Sigma Pictures uses automated cataloguing processes and provides a simple yet feature rich interface to search, view and export images stored within HPE CM.
Streamline Cataloguing
Normally importing images into HPE CM is a time consuming process with each image requiring the user to enter the relevant metadata and manually create a record for each image. With Sigma Pictures users can preview then import pictures directly from cameras, phones or file folders and network drives. Simply select a pre-configured cataloguing wizard to initiate the process and let Sigma Pictures do the hard work.
The step-by-step wizards are configured by your Administrators to ensure all the required metadata is captured during the cataloguing process. This streamlined cataloguing process provides a consistent image naming convention improving search and retrieval.
Rapid Search
Sigma Pictures utilises the standard HPE CM search dialogue familiar to all HPE CM users. Search results are displayed in either list, small or large thumbnails, film strip, preview or scrolling 3D view. The thumbnails displayed are also stored in HPE CM as renditions of the original picture. These are generated at import time or by retrieved search results and are automatically saved in HPE CM for future use.
During the import process Sigma Pictures extracts common EXIF metadata from photos and stores HPE CM fields including GPS co-ordinates. This information can be used to automatically catalogue images into HPE CM based the location of where the photo was taken or perform searches for other geotagged images. For example, users can search for all images within a specific radius of a particular image, generate a KML (Keyhole Markup Language) file from the search results and have it automatically open in the default viewer application (for example, Google Earth).
Barcode Cataloguing
Sigma Pictures is able to separate batches of photos using barcode recognition. Sigma Pictures can detect and decode barcodes from within photos and use this metadata to determine the location for the images that follow. For example, a camera operator can take a picture of a barcode (HPE CM record/container number) and Sigma Pictures will detect this barcode and automatically catalogue the images following into the associated HPE CM file.
Image Processing
Sigma Pictures can be configured to apply various image processing techniques during the import process. Features include image resize, compression adjustment, conversion to JPG or TIF, auto-rotate, add a border or add a text stamp (eg Record number). For example, these functions can be used to create resized web versions of images, reduce the overall file (storage) size or add copyright details and other text onto the border of the photo.
Thumbnail Renditions
You can generate thumbnails for documents already in TRIM, Records Manager or Content Manager. This function is useful to process images that were previously added to the dataset using methods other than Sigma Pictures.
Metadata Mapping
The Metadata Mapping wizard is used to configure how and where metadata is captured about each image that is imported. It also controls where the image is filed. This wizard enables the administrator to configure complex nested rules for the construction and format of metadata. All metadata items may also be mapped to prompts, which will appear in the wizard when the user begins an import. Pictures imports allow for all standard EXIF metadata to be sourced, as well as Dublin Core and IPTC sources.
Reporting and Exporting
Sigma Pictures features several out-of-the box reports based on search results. Including thumbnail reports, four images per page or one image per page. Sigma Pictures can also generate a KML (Keyhole Markup Language) file from the search results and have it automatically open in the default viewer application (for example, Google Earth) or select images to display overlayed on Bing Maps. Advanced integration with 3rd party Geographic Information Systems (GIS) is also available.
Administration and Security
The cataloguing wizards accessible to users are controlled by Records Administrators and automatically published for user access. By using intuitive template-based metadata mapping Records Administrators can create multiple cataloguing wizards to streamline the importing of pictures for different use cases. These cataloguing wizard configurations are stored inside the HPE CM dataset – meaning users all share the same configuration settings. Administrators have complete user access control over which cataloguing wizards or software features are available to HPE CM users. All of these settings are managed and stored within HPE CM.
Sigma Pictures is a mature product that set the industry standard for image management with TRIM, Records Manager and Content Manager. The product continues to be developed ensuring compatibility across all supported TRIM, Records Manager, Content Manager and Microsoft Windows versions.
Our standard software support agreement includes access to the support help desk and new versions of the product as they become available.


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12.19.2014: Rainy. Cloudy. Windy. Sad time. Said final farewell to a childhood friend Edna Aniana Cruz who passed. I see the image of her on Edlyn, her only child.
I said in my eulogy:
"Edlyn, this is a very difficult time for you and your family. Your mom's sudden passing is truly heartbreaking that touched the hearts of relatives, friends and kab…abayans. Both your mom and dad are my classmates. I am joined by classmates from grade school and high school in offering our sincere condolences. Your mom was a wonderful friend to all of us and remembered for her kindness, humility and compassion. We personally have many fond memories of our friendship that goes back to our grade school days. Edlyn, as your mom joins your dad, we thank her for those memories. Those memories will forever be imprinted in my heart and of our classmates. May she rest in peace."
She and her husband Uleng were my classmates. Sadly Uleng passed away too soon. When I talked with Edna our conversation always included the regular after-school trips to their home where her mother prepared snacks for us. Her parents were kind and hospitable not only to Edna's classmates but to those of Lito, Chabeng and Lyn's.
Edna is remembered as a humble, generous and very compassionate person. When I called to ask how she was, there was always this positive note, then changed the conversation to ask about me and our classmates. Inviting her to a gathering, she was excited to go. Our classmate Mel Tunque and his wife Ludy who live close by were the regulars to give Edna a ride!
I can feel now that losing a childhood friend and classmate is life changing. There will no longer be that time to dial the phone for a brief, turned-lengthy, conversation at a given moment. Gone is the reality to see her name included in our active roster to invite at some reunion, community event and to ask for a donation. When asking Edna for a donation her answer is pre-printed as a YES, cast in stone! She is that kind of a person with a heart filled with generosity.
Her sister Chabeng and nieces Mayco, Majoh and Minnie are grieving yet thankful to have spent 24 glorious days with Edna this past November after over twenty years. That was a gift like no other. My heart, the hearts of our classmates, relatives and friends are broken over losing such a wonderful person, yet letting go so Edna can finally be reconciled with her husband Uleng.
As the page is turned, a new chapter appears in the midst with Edlyn, her husband Baldwin, their children Madelyn and Brooklyn. Edna's cellphone no. remains on mine to revisit the childhood days from time to time.
With loving thoughts,


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Dj Nahum Ray Transforms With Bumble Bee
So I took a trip to L.A. to visit the Dream Center and who did I run into in Hollywood? None other than Bumble Bee from the movie Transformers. One of my favorite Transformers and such a nice Transformer. He only asked me for a tip to take a picture with him. I gave him one. I told him to tune his radio in to 107.3 KFFM to listen to all of todays #1 Hit Music. Now Bumble Bee is listening!


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'Smashed' Director to Helm Young Adult Adaptation 'Pure'
While at Sundance to premiere his new film, it's been announced that writer and director James Ponsoldt will write and direct the upcoming big screen adaptation of the young adult fiction novel 'Pure.'
THR reports that James Ponsoldt has been hired to write and direct 'Pure,' based on the young adult fiction novel of the same name by Julianna Baggott. The story takes place in a post-apocalyptic world (where all young adult novels must take place because teenagers are always like, "the world is ending!" and writers like symbolism) where the human race has been divided by a dome -- the "Pures" live under the dome and they're very pretty and everything is amazing for them, while the "Wretches" live outside the dome and are scarred for life. Again, symbolism.
But then a Wretch girl goes on the run with a Pure boy who happens to be the son of the Pure leader because Romeo and Juliet.
Ponsoldt launched his indie drama 'Smashed,' starring Mary Elizabeth Winstead and Aaron Paul, at Sundance last year and he's back this year with his follow-up, 'The Spectacular Now,' which premieres tomorrow. That film stars Miles Teller and Shailene Woodley, and is Ponsoldt's third movie in a row to play Sundance.


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Building a Design Firm's Professional Development Program - Business Development
You can refer to this section as business development or client facing skills since few firm leaders consider that they include their in-house professional development programs as a part of their marketing and promotion efforts. Professional development within A/E firms has evolved during the past decade and it is time to re-think how A/E firms share and distribute their intellectual property with professional associations and clients. For those firms that already have in-house programs you likely already have components in place. I offered several suggestions last November in my blog Overlooked Internal Training Sources for A/E Firms.
Business development (marketing and promotion) is a critical element of every firm. If your professional development program is intended for internal use, then be sure that your marketing plan relates to the needs assessment of your staff and client's knowledge needs. If the firm includes education as a part of external marketing efforts be sure that it is also included within the firm's strategic plan for educating target audiences. A staff presentation at an industry conference is a good example. You may have the world's most knowledgeable subject matter expert (SME), designed an interesting presentation, and even offered the program using an innovative delivery format. However, if the intended audience is not aware that course is being offered then be surprised at a low turnout. Those who rely solely on the firm's reputation to spread the word will frequently fail. You must adequately promote and advertise each of your courses. Budget accordingly.
Continuing in part six of this eight part series I have assembled requirements from several prestigious organizational award programs that appear with consistency. The self-assessment presented in this piece are intended to assist those individuals who are responsible for shaping and managing the organizational structure of an Architectural/Engineering Design and Consulting Firms' professional education department. This assessment tool is not intended for the design or development of any individual course, certification, skill, or professional program.
KEY 5: Business Development (Marketing and Promotion)
Key 5 examines the firm's business development structure that includes marketing and promotion of the educational courses and programs. This section provides recommendations for how the firm should address both internal and external marketing and promotion or their education courses and programs.
1. There is an established long-term educational marketing plan in place that includes: budget and pricing; projected incomes (including internal between departments); registration and enrollment procedures; number of classes and class sizes per session; cancellation policies; fees (ex: staff, instructor, course development expenses; course materials, equipment, technical considerations, facilities).
2. There is a separate One-Year marketing plan.
3. Print and social media promotional and advertising methods are used to support the marketing strategy that includes related expenses.
4. Other promotional activities include publicity, advertising, open houses, press releases, etc. to clients supporting speakers at professional conferences.
5. Quantitative metrics are in places that measure indicators and provide current levels, trends, and any appropriate comparative data.
6. There is a process for projecting new educational activities.
7. The marketing plan and promotion efforts are evaluated for effectiveness annually.
8. A process for researching the regulatory standards and legal and ethical requirements that should be addressed through professional development.
9. A process for ensuring that the firm addresses its responsibilities to the client, the profession, and the community through community outreach through education and training.
Using the Baldrige National Quality Award and IACET as models, a special task force created the AIA/CES Award for Excellence for The American Institute of Architects, Continuing Education System. This program was used as a cornerstone for building a national continuing education program that shaped education offered in the design industry. Today, other learning and development award programs such as the ASTD-Awards/Best-Awards and the Chief Learning Officer, CLOmedia Awards are also being used to elevate the practice of learning and development.


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With Christmas just round the corner this week's big DVD release is the latest superhero reboot: Fantastic Four. Should it find a place under your Christmas tree?
The movie follows young genius Reed Richards (Miles Teller) as he pilots the development of an inter-dimensional teleporter. He teams up with friend Ben Grimm (Jamie Bell), siblings Johnny and Sue Storm (Michael B Jordan and Kate Mara) and the unfortunately named Victor von Doom (Tony Kebbell).
Naturally the experiment goes wrong and the youngsters are granted the powers which transform them into Mr Fantastic, The Thing, The Human Torch, The Invisible Woman and Dr Doom. It shouldn't be too difficult to pick out the bad apple in that bunch and it isn't long til Doom is trying to make his name a reality.
It's not been that long since these Marvel superheroes were last on our screens. Their previous outings came in a couple of light-hearted romps back in the noughties but the shift in tone here couldn't be more stark.
Director Josh Trank has taken a more sombre approach, creating a decidedly downbeat movie which often feels dull. At 90 minutes it's a short film by modern standards but a lot of that is spent with these gloomy youngsters mooching around doing not very much.
The worst thing is that nobody is really having any fun. You can make superhero films serious, but stray too far from the comic roots and it's hard to keep things credible.
With a lack of any real action in the first half the film comes very close to stalling before things finally kick into gear in the last act when effects-fuelled battles inject a little pace into the hitherto drab proceedings. But by this point it's a little too late, and the Fantastic Four has failed to live up to the name.


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We are glad to announce that DJ Galactic's song has been selected by Playlist 7 and Microsoft, to be part of sponsoring progam.
The song Orbital will be feature 1 week at PLaylist 7 for free streaming and download.
You can come to support DJ Galactic by visiting Playlist 7 and download a free Mp3 of Orbital.
You can download Orbital here
Thank you for your support


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Promote your payday loan business online with our payday loan marketing funnel landing page.
Capture potential leads online with our appealing and professional payday loan lead capturing funnel landing page design. Payday loan funnel lead capturing landing page design consist of funnel main page with call to action button, funnel lead form page and a thanks page.
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Payday loan lead capturing funnel main page design
Continue reading Payday loan lead capturing funnel landing page


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I am a lover of all things creative. But I have a stronger passion for things that are homemade & of natural texture & composure. I also fall in love with grouped items of the same natural feel. I especially like it when that grouping is a larger one consisting of soaps, dishes, jellies, coffee, purses, scarves, jewelry and other homey goods. SO when I came across Shoppe by scout mob I was pleasantly surprised because it was as if it had been tailored exactly to my liking. I loved everything. In fact I have quite a good many christmas present ideas just from their site. Below I have included photos of a few diverse items on their site & I hope you enjoy this shoppe as much as I do. Each photo links to the specific page on which you can purchase that individual item from the shoppe. I hope you LOVE IT as much as I do!
*disclaimer* none of the pictures from this post are my own!


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Learn more about our services and contact for a quote.
Flashporter testimonial
Product promo for a photo backup device using real-life customers and authentic storytelling. Producer/ director: Christine Ren; Filming/ edit: Christopher Chandler
Flashporter promo
Product promo for a photo backup device using real-life customers and authentic storytelling. Producer/ director: Christine Ren; Filming/ edit: Christopher Chandler
LivingHomes Indiegogo
Livinghomes is building a new, low-cost, prefabricated, net zero electricty LivingHome for Make It Right that will be a showcase for top sustainable materials, technologies and design strategies. It will debut October 23-24 at Greenbuild, the largest conference in the world for sustainable design, and then it will be permanently installed at Make It Right's community in the Lower Ninth Ward in New Orleans, where a deserving family will be selected and will one day call it Home Sweet (Living)Home!Help support the LivingHome for Make It Right in New Orleans at indiegogo.com/projects/we-re-building-a-livinghome-for-non-profit-mir.Produced & directed by: Christine Ren Videography: Greg Armstrong Editing: Greg Armstrong
2RedWinery Indiegogo
You expect your wine to be satisfying. To complement the moment, the meal and the company. But, what if you wind could do more? Introducing 2RedWinery, a line of award-winning wines committing 100% of its profits to supporting prostate cancer research through the registered 501(c)3 non-profit, The Robert and Susan Hollander Foundation. Learn more at 2redwinery.com.Help 2RedWinery make their next 2 barrels of wine. Support the Indiegogo campaign at igg.me/at/2red.------------------------------------------------------------Produced, filmed & edited by: Christine Ren
Promise by PUE Kickstarter
John Collins, a Liberian refugee, shares his compelling story and the impetus launching his brand, PROMISE by PUE. His new collection features beanie hats, designed with the colors and flags of every country in the world.Please back the campaign today at: kickstarter.com/projects/promisebypue/the-worlds-best-beanie-promise.A short narrative excerpt from John: "When I came to this country 18 years ago... fleeing a civil war in Liberia; I made a promise.A promise not to be ashamed of where I came from.That place, Liberia, is a part of my story...and in this life, the only thing you can beis yourself."------------------------------------------------------------Produced, filmed & edited by: Christine Ren
Stretch Your Horse Kickstarter
New science suggests that, for optimal performance, horses need stretching (just like us!) Introducing "Stretch Your Horse;" a mobile app for Android and iOS (Apple) smartphones featuring 24 individual instructional horse stretching videos."The videos put scientific, practical, easy to follow information directly into the hands of horse owners all over the world to help them keep their horse healthy, sound and performing to the best of their ability regardless of their riding discipline or experience level," states Dr. Jack Root, DVM, owner of Oakhurst Equine Veterinary Services and owner of Grindstone, 1996 Kentucky Derby winner.Support the Kickstarter campaign at kickstarter.com/projects/stretchyourhorse/stretch-your-horse-app-24-individual-instructional.Learn more about the founder, Ilene Nessenson, of Holistic Horse Bodyworks LLC at holistichorsebodyworks.com.Produced by: Christine Ren Filmed & edited by: Justin Bench


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Mynewsdesk Newsroom - Product Factsheet (Japan)
Document • Nov 25, 2013 17:35 +08
|Find out more about how Mynewsdesk can help you. ■ Benefits & Functions of Mynewsdesk ■ A must-have for Brand and Product Owners, those in Public Relations, Marketing & Corporate Communications. Useful for Inbound Marketing, Social Media & Content Strategies, Brand Journalism, Influencers & Investor Relations, Crisis communications, International communications, Government & University PR.
|Creative Commons Attribution (?)