text:
Check Amazon.com for a Russian vpn trial Deal. Just click the button on the right and you can immediatly see what's available.*As an Amazon Associate I earn from qualifying purchases.
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text:
Nic Denson - Head of PMO, Information Technology, British Heart Foundation
Nic has very quickly proved himself to be a talent worth keeping an eye on. After three years as a project manager at BAE Systems, Nic joined the University of East London as an IT Project Manager, rising to Deputy Head of PMO shortly afterwards. Since then, he has joined the British Heart Foundation, where he currently holds the Head of PMO position.
Top 250 UK IT Leaders
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The UK IT Industry Awards are back for 2020!
Entries are open for the industry's most prestigious awards.
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The top-ten tenets of software quality assurance, part six: testing
Testers have to be destroyers, argues Mark Wilson. But too often testers aren't trying too hard to bust the software. Rather, they are trying to show it working
Coming soon, save the date: Deskflix, Computing's online event series
Season One: Empowering the remote working revolution

subset: commoncrawl


text:
Enter client first name, email, and the MMS name.
This will be used in the email sent, i.e. 'Hi %FIRSTNAME%, We need feedback on the experience %MMS_NAME% has had with Roobix.'
Take advantage of a free 30-minute consultation.Lets us know a time that works for you and we'll be in touch.
Western AustraliaLevel 2 / 541 Hay St, Subiaco WA 6008
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text:
« ZurückWeiter »
on ALL subjects of LITERA-
oRIGINAL LETTERs, ANEcDoTes,
LITERARY And PHILOSOPHICAL
on 161NAL LETTERs of LADY MARY wortley Montagu, addressed to MRs. (Miss) ANNE J USTICE, upon the PAveMENT, Yost K. Printed from the origiNALs.
I. sure, dear Nanny, you'll excuse my silence this bout: this last fortnight has been wholly taken up in receiving visits of congratulation upon my brother's wedding. My new sister is to passe the summer in the house with me, so you may be sure I shall have very little time to my selfe. I am perfectly ignorant of the marriage you mean, and so dull I can't guesse the name of the lord whose character you say is so good. If you are not at Scoffton this summer, I must despair of seeing you. I fancy about the latter end of this month we shall be going into Nottinghamshire. I writ to Mrs. B. three or four posts ago, and told her I heard she was going to be married; and gave her good advice, to forget Mr. Vane and take the first lover her relations propos'd to her—pray write me word if she follows it. I allwaies wish her very well. The small-pox rages dreadfully, and has carry'd off several people here: that, and the heat of the weather, makes me wish myselfe in the country. My eyes are something better, for I was not able to write for a good while; but they are still weak, and make me, sooner than I otherwaie would, tell you, that I am, dear Nanny, Your's to serve you.
June 5.
Ay, ay, as you say, my dear, men are vile inconstant toads. Mr. Vane could never write with the brisk air if he had any sorrow in his heart; however, the letter is realy pritty, and gives me a good opinion of his understanding, tho' none of his fidelity; I think they seldom go together. You are much in the right not to undeceive Mrs. B. I would not have her know any thing to vex her, as such a piece of news needs must. Poor lady!—but she's happy in being more discreet than I could be. On the other
Monruly Mac, No. 181.
hand, I could beat Mr. Vane, as much a pritty gentleman as I hear he is. . I'll swear, by his letter, he seems to have more mind to rival Mr. Crotchrode than break his heart for Mrs. B. I shall neither see dear Mrs. Justice, nor any of my north country friends, this year. I'm got into the west, over the hills and far away. Here is nothing to be lik'd that I can find; every thing in the same mode and fashion as in the days of king Arthur and the knights of the round table. In the hall, a great shovel board table and antick suits of armour; the parlor furnish'd with right reverend turky work chairs and carpets; and for books, the famous History of Amadis de Gaul, and the Book of Martyrs, with wooden cuts; and for company, not a mortal man but the parson of the parish, some fourscore or thereabouts: you know I was never a violent friend to the cloth, but I must make a virtue of necessity, and talk to him or nobody. This is the present posture of my affairs, which you must own very dismal. Times may mend; there is nothing sure, but that I am your's.
Direct for me at West-Dean, to be left at Mr. Foulks, a coffee-house, at the Three Lions, in Salisbury, Wiltshire.
The paper I mention'd is very long, and I don't know whether you'l think it worth postage; but if you persist in desiring it, I'll send it you. June 14. To Mrs. Anne Justice, York.
Not HING could be more obliging than so quick a return to my letter, and sending what I enquired for. I pity your poor Strephon, and guesse what effect such a letter must make on your heart. I like of all things his manner of writing, and am sorry all your wishes are not successful. Mr. V- has been a great dissembler if it breaks off of his side; but 'tis hard to distinguish false love from true. The poor lady is in a sweet pickle; and I am so good-natured to be sorry for all prople who have misfortunes,especially, of that kind which I think the most touch
ing. I would to God I was with you reading the Atalantis' I know the book, and 'twould be a vast pleasure to me to read some of the storys with you, which are realy very pritty: some part of Eleonora's I likc mightily, and all Diana's, which is the more moving because 'tis all true. If you and I was together now we should be very good company, for I'm in a very pritty garden with a book of charming verses in my hand. I don't know when we shall see Mrs. B. but when we do come into that country, is it quite impossible for you to stay a week or so with us?. I only hint this, for I know people's inclinations must submit to their conveniencys; only tell me how far it may be possible on your side, and then I'll endeavour it on mine; though a thousand things may happen to make it impossible as to my part. You know you should be allwaies welcome to me, and 'tis none of my fault if I don't see you. Remember your promise concerning the letters. To Mrs. Ann Justice, at York.
YES, yes, my dear, here is woods, and shades, and groves, in abundance. You are in the right on't; 'tis not the place,
but the solitude of the place, that is in
tolerable. 'Tis a horrid thing to see nothing but trees in a wood, and to walk by a purling stream to ogle the gudgeons, in it. I'm glad you continue your inclination to reading; 'tis the most improving and most pleasant of all employments, and helps to wear away many melancholy hours. I hear from some Nottinghamshire people, that Mrs. B. is not at all concern'd at the breaking off her match. I wonder at her courage if she is not, and at her prudence in dissembling it if she is. Prudent people are very happy: 'Tis an exceeding fine thing, that's certain; but I was born without it, and shall retain to my day of death the humour of saying what I think; therefore you may believe me, when I protest I am much mortify'd at not seeing the North this year, for a hundred and fifty reasons; amongst the rest, I should have been heartily glad to have seen my Lord Holdernesse. In this hideous country 'tis not the fashion to visit; and the few neighbours there are keep as far from one another as ever they can. The diversion here is walking; which indeed are very pritty all about the house; but then you may walk two mile without meeting a living creature but a few straggling cows. We have been here near this month, and seen but one visitor, and
her I never desire to see again, for I never saw such a monster in my life.
I am very sorry for your sore eyes. By this time I hope all's over, and you can see as well as ever. Adieu, my dear. When you drink tea with Mrs. B. drink my health, and do me the justice to believe I wish my selfe with you.
July 7.
To Mrs. Anne Justice, York.
IAM very glad you divert yourselfe so well. I endeavour to make my solitude as agreeable as I can. Most things of that kind are in the power of the mind: we may make ourselves easy, if we cannot perfectly happy. The news you tell me very much surprizes me. I wish Mrs. B. extremely well, and hope she designs better for her selfe than a stolen wedding, with a man who (you know) we have reason to believe not the most sincere lover upon earth; and since his estate is in such very bad order, I am elearly of your opinion, his best course would be to the army, for I suppose six or seven thousand pound (if he should get that with his mistrisse) would not set him up again, and there he might possibly establish his fortune, at least better it, and at worst be rid of all his cares. I wonder all the young men in England don't take that method; certainly the most profitable as well the noblest. I confess I cannot believe Mrs. B. so imprudent to keep on any private correspondence with him. I much doubt her perfect happiness if she runs away with him. I fear she will have more reason than ever to say there is no such thing. I have just now received the numbers of the great lottery which is drawing: I find my selfe (as yet) among the unlucky; but, thank God, the great prize is not come out, and there's room for hopes still. Prithee, dear child, pray heartily for me. If I win, I don't question (in spite of all our disputes) to find my selfe perfectly happy. My heart goes very much pit-a-pat about it; but I've a hor: rid ill bodeing mind, that tells me I shan't win a farthing. I should be very very glad to be mistaken in that case. I hear Mrs. B. has been at the Spaw. I wonder you don't mention it. Adieu, my dear. Pray make no more excuses about long letters, and believe your's never Seem SO to me. August 7.
To Mrs. Anne Justice, York.


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text: Covington Chronicle Mrs. Covington's Third Grade News Week of January 4th A note from the teacher: I hope you all had a fantastic holiday and I wish you all a very Happy New Year! We are ready to rock 2016! Learning Targets This Week Math: *I can apply properties of operations as strategies to multiply and divide. (3.OA.5) Examples: If 6 × 4 = 24 is known, then 4 × 6 = 24 is also known. (Commutative property of multiplication.) 3 × 5 × 2 can be found by 3 × 5 = 15, then 15 × 2 = 30, or by 5 × 2 = 10, then 3 × 10 = 30. (Associative property of multiplication.) Knowing that 8 × 5 = 40 and 8 × 2 = 16, one can find 8 × 7 as 8 × (5 + 2) = (8 × 5) + (8 × 2) = 40 + 16 = 56. (Distributive property.) We are quickly approaching the second half of the school year and students are participating in many programs and activities to help them become stronger readers. RTI groupings are in full swing and all students are getting what they need to push them to the next level of greatness. Please continue to read with your child each night and hold them accountable by asking them to summarize or ask them questions about what they have read. Over the next few weeks your child will be retested on fluency, accuracy, and reading comprehension and I am excited to see how much they have grown! Science: *I can compare Earth's saltwater and freshwater features (including oceans, seas, rivers, lakes, ponds, streams, and glaciers). (3.E.2.1) *I can compare Earth's land features (including volcanoes, mountains, valleys, canyons, caverns, and islands) by using models, pictures, diagrams, and maps. (3.E.2.2) January 8th: School Spelling Bee January 18th: No School/ Martin Luther King, Jr This week students will be assessed on their oral reading fluency, oral comprehension, and written comprehension. Students are encouraged to do their best to show how much they have improved with their reading. We have been working hard on our fluency with our "Six Minute Solution" program and have been answering written prompts in our guided reading groups. Students are ready to rock the test! January 19th-20th: Teacher Workdays (No School) January 28th: Report Cards go home


text:
The Classe Collection Deluxe 7
Apartment in Around Antalya
Image by Booking.com
Information from Booking.com
Quick description
Within 2.4 km of Serik Belek Public Beach and 7 km of The Land of Legends Theme Park, The Classe Collection Deluxe 7 features free WiFi and a garden. The air-conditioned apartment consists of 3 bedrooms, a kitchen with dining area, and 4 bathrooms with shower. Garden of Tolerance is 6 km from the apartment. The nearest airport is Antalya Airport, 35 km from The Classe Collection Deluxe 7.
- Garden
- Internet services
- Wifi
- Wifi available in all areas
- Safety deposit box
- Free WiFi available


text:
Viewing Listing MLS# 522605
No Recent Price Change
40 Sin Salida
Sedona, AZ 86351
- Residential
- Townhouse
- Pending - Take Backup
Agent Remarks
Scarce, desirable, 3-bedroom, 1910 sq. ft. single level, floor plan! Las Piedras is the favored patio and townhome community in the Village of Oak Creek. 12'' ceilings welcome you upon entry. This home features a spacious kitchen with center island, maple cabinets, breakfast bar and casual dining area. This split-floor plan offers 3 bedrooms, 2 baths, a generous master suite, with large walk in closet, separate tub and shower and access to the private back yard. Exceptional property for those looking for minimum maintenance as the HOA fee covers trash, roof, exterior stucco, some street sealcoating, front yard & common area landscape maintenance. This home is centrally located to all that the Village of Oak Creek has to offer - shopping, golf, hiking & dining!
Building Info
Year Built: 2,001
Construction: Stucco, Frame - Wood
Floor Plan: Open/Modern, Split Bedroom, Great Room
Floors: Carpet, Tile, Wood
Foundation: Slab
Handicap Features: None
Internal Amenities: Garage Door Opener, Smoke Detector, Fire Sprinklers
Levels: Single Level, Level Entry
Roof Materials: Tile
Exterior Features
Flood Zone: Verify
Asking Price Text: $469,000
Interior Features
Kitchen Features: Gas, Breakfast Bar, Island, Pantry
Lot Info
Acres: 0
Road Access Type: County
Views: Red Rocks/Boulders
Misc Info
External Amenities: Open Patio, Landscaping, Pool, Community, Covered Patio(s)
Mobile Home Type: None
Pet Privileges: Domestics
Other Rooms Info
Dining Room Features: Kitchen/Dining Combo
Living Room Features: Cathedral Ceiling, Ceiling Fan, Great Room
Parking Info
Parking: 2 Car, Off Street
Property Features
Basement: None
Fireplace: Gas
Style: Southwest, Contemporary, Spanish
Property Info
County: Yavapai
Class Type: Townhouse
Buildings: None
Homeowners Warranty: None
Irrigation: None
Location: Mountain Views, View
Road Maintenance: County Maintained
Room Features
Beds: 3
Rooms Info
Master Bedroom: Walk in Closet
Other Rooms: Laundry
Sale / Lease Info
Special Conditions: N/A
SqFt Info
Sqft: 1,910
Unit Info
Appliances Included: Refrigerator, Disposal, Washer, Dishwasher, Microwave Built In, Dryer, Wall Oven, Cooktop, Gas Oven
Utilities / HVAC
Cooling: Refrigeration/Centrl, Ceiling Fan
Utilities Included: Cable TV, Underground, Sewer (Private), Electricity, Private Water, Telephone, Natural Gas
Water Heater: Natural Gas
Courtesy of Arizona Prime Real Estate


text:
Q. Hello Daniel, thank you for your time in taking this interview. Can I start by asking why you decided to write your first book?
A. It was trapped in my head. I had to let it out. If not it would have eaten my brain.
Q. How long have you been writing?
A. On and off my whole life. However, for that past two years, I have nearly written every day.
Q. How long did it take you to write this book?
A. It took a year. I work full time so I would write at odd hours.
Q. What do you use to write your books?
A. I start with a pen and notebook. Will fester in there for a bit then move on to a PC. Also, I drink a lot while I write, mainly wine and beer.
Q. What problems did you encounter?
A. Whenever I would get stuck I'd get pretty lit on vodka or rum. That seemed to always get the cogs turning. Oh yeah, I'd blast Led Zeppelin.
Q. How do you think you've evolved as a writer since you've started?
A. I've learned how to give each character their own voice. I write up a lot of backstory for all the characters. Small details that may never make it into the actual work. This makes each person more real, more unique.
Q. Is there anything you wished you'd known when you started writing?
A. That grammar doesn't matter. That can be fixed. Just focus on the story.
Q. Do you structure your plots or just go with the flow?
A. I always know where the story is going to end up. It is the small details I may make up as I go along.
Q. Do you work on a set amount of words per day or does it change?
A. No, I cannot do it that way. I get too crazy about hitting the mark. I just work on scenes.
Q. Do you do a lot of research when writing a book?
A. Yes, if it is needed. I will find an expert in regards to the topic I need information on. I will then run the scene by them. Tell them what I need to accomplish in it. I will have them ensure that it is being written in a way that it would occur in real life.
Q. How would you describe your writing process?
A. Organized, methodical, and intoxicated. After I get out of the notebook and into the PC I may spend days on a couple lines. When I am done a chapter I print it off, reread it, and then write it again.
Q. What time of the day do you find is best to write?
A. I prefer during the daytime, I'm not exhausted. But due to my life situation, I normally write very late at night.
Q. Where do you draw your inspiration from?
A. Real life. I've had a pretty crazy life so far, and have befriended many interesting people. I use all of that to churn up what I feel are some fun ideas to write about.
Q. What draws you to this genre?
A. Because it is real or at least plausible that it could happen. I'm not able to suspended reality inside my head to write science fiction books. I enjoy writing scenes, stories that could have or have occurred in real life. Many of the things I have written have a lot of truth to them.
Q. Have you ever tried to write other genres?
A. Yes, I have. Science fiction fantasy type pieces. It did not go well. My kung fu is weak in those areas.
Q. Which author/book would compare yours too?
A. I think such a question is best left up to people who have read my work.
Q. Can you relate to any of your own stories?
A. Almost every part of my writing has an anchor in my life. The opening scene in my short piece Broken is exactly how it happened in real life. You'll haft to read it to find out more details.
Q. How many books have you written?
A. Three, but only published one.
Q. Have you ever written in collaboration with another author?
A. No, and I have no desire to.
Q. Who designed your front cover?
A. Haha. I do, that is why they suck. I'm trying to avoid the money pit that is self-publishing.
Q. Who was the first person you showed your novel too?
A. My wife. But then to an ex-girlfriend. Figured the scorn would motivate her to be honest.
Q. How do you market your books?
A. Poorly. I have no illusions that I'm going to make it big in this whole game. Just keeping it all organic.
Q. How do you deal with bad reviews?
A. Hmmm? I feel bad that they don't understand they just read something really good.
Q. How much time do you devote to marketing your books?
A. Little. I do the twitter thing a bit. Really my using EBOOKBUMP is my first real attempt at marketing. Like I said before, just keeping it organic.
Q. How do you get your book reviews/reviewed?
A. Just wait for people to leave them on Amazon.
Q. Do you do all your own proofreading and editing?
A. Hell no. That is the one thing I spend money on when it comes to this whole mess.
Q. How and where are you publishing this book?
A. Just on Amazon for now. Back in the day, I would send work out to small magazines. But I never really cared for that or their opinions on my work. Years ago a small magazine wanted to publish a short story of mine but wanted the ending changed. Screw that noise.
Q. What are the main benefits of being an independent author?
A. I can tell people to go to hell. This whole show is mine. Even with Amazon, if I get sick with their games I'll just take my book down. I don't care about being famous or rich, just telling the story the way I want to.
Q. What are you reading at the moment? Which book do you have by your bed?
A. A detailed history of ancient Japanese culture, Lawrence of Arabia's book the Seven Pillars of Wisdom, and Neil Degrasse Tyson's Astrophysics for People in a Hurry.
Q. Who is your favourite author/book?
A. Hemingway, his short story The Killers is perfection.
Q. What is your favourite quote from a book?
A. Just read Fight Club, it is full of amazing quotes.
Q. What is your favourite book to film adaptation?
A. The Thin Red Line
Q. Where are your favourite places to read?
A. In the car. I like being alone, the solitude.
Q. What books do you read to your children?
A. A lot of Doctor Seuss.
Q. When you read do you prefer a book or a Kindle/tablet?
A. A book, but still feel free to enjoy my book on your Kindle or tablet.
Q. Do you have any tips for aspiring authors?
A. Ignore the self-doubt and write every day. Oh yeah, just do it for yourself.
Q. What's coming next?
A. That is a secret. But, the next one even walks the line that much closer to my real life experiences and fiction


text:
Posted my last entry to see that it is in paragraph format! WTF? Went back and edited older posts into paragraphs so as not to be accused of damaging the eyes of anyone who happens to read my drivel!
A little while back, I started trying to (unsuccesfully) paint black. Thread found here:
As Dragonsreach was kind enough to point out, I was following a technique for larger scale minis that did not translate well to 28mm. So based off the recommendations of a few forum members and youtube reviews, I ordered the Andrea Black paint set from The Michigan Toy Soldier Company: http://www.michtoy.com
Originally Posted by TOPC Gallery
Just finished this prototype for 2 huge blood angles armies I will be painting.
Wanted a smooth and atmospheric look that i could achieve with no hassle in just a couple steps.
I was going for simple here as I will be hammering up to 20 of these a day.
Also this was the first test for my new photo booth
looking forward to the full army
comments and criticism welcome
LINK - http://i45.tinypic.com/jafaee.jpg
Well i painted up my first mini of my grey knights, and as the poster boy for the army i chose a halberd wielding terminator.
Using a guide from Garfy on Tale of many painters Blog i ended up with this:
Quite liking the blue note to the armour, but i have seen another way of doing the armour that has a more grey look.
Will update once i have completed the other great task, my space wolves
Well its been...erm...a while since i posted here.
TBH i had forgotten about it as life got in the way and i went througha bit of a bad time.
All is well now, and i have got back into the swing of things, i hope to be more active here and start doing some progress for my build up to entering my first golden demon at this years UKGD.
I will try and teach myself to use the blog a bit more and try to update regularly.
My version of BA Fellblade. Lighted (9V battery), antenas are magnetized and sponsons are posible to remove. More photos on http://tauempire.blogspot.com
Work in progress for a regular client .i will have some snow in the crevices and tracks by the end which I am looking forward to doing Enjoy !All C&C welcome.I do take commissions LINK - http://i48.tinypic.com/t630q0.jpgZachary AndrewsTOPC email@example.com://www.facebook.com/topcmini
A few minutes ago, I posted my first ever Blog. I typed out my thoughts in lovely paragraph format only to have the post crunched together into one large paragraph. Did I violate some sort of Blog rules by using proper writing format? Is the Blog the death of the written language as we know it, or is it one of the signs of the Mayan Apocalypse?
Updated 05-01-2012 at 12:32 PM by Bloodhowl
I learned recently that a large tax refund, high-speed internet access and copious amounts of alcohol, may not be the best of combinations. I awoke the next morning after combining the three to find that in addtion to a few other items, I had ordered not one, but three finecast miniatures from Games Workshop. Fortunately, the models fit into the Tomb Kings army I have been collecting over the past several years. I had ordered the High Liche Priest, Prince Apophas, and the Necrotect. She-Who-Must-Be-Obeyed
Updated 05-02-2012 at 12:59 PM by Bloodhowl
Just getting started here, but I have my own blog that I update sporadically (trying to make it more consistent) at daybydayminis.blogspot.com. Hope to hear from everyone!
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Input your desired command into the text area. All Marine Corps, Coast Guard, and Navy logos- NUCONs, DECOMs, & everything in between- are available. Select a monochrome or full color crest. Obtain approval from command when worn under MWUs. Official US Navy Product.
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Microcin J25 (MccJ25) is a 21-residue ribosomally synthesized lariat peptide antibiotic. MccJ25 is active against such food-borne disease-causing pathogens as Salmonella spp., Shigella spp., and Escherichia coli, including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative. In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica serovar Newport and Escherichia coli O157:H7 in skim milk and egg yolk. However, unlike the wild-type MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing its potential use in food preservation.
Escherichia coli microcin J25 (MccJ25) is a plasmid-encoded antibiotic peptide consisting of 21 amino acid residues (G1-G-A-G-H5-V-P-E-Y-F10-V-G-I-G-T15-P-I-S-F-Y20-G) (4, 12). Four genes (mcjA, mcjB, mcjC, and mcjD) are required for MccJ25 synthesis, export, and immunity (14, 15). The mcjA gene encodes a 58-amino-acid MccJ25 precursor, which is processed by the products of mcjC and mcjB (7). Once synthesized, the mature MccJ25 is excreted to the medium by McjD, an ABC-type transporter (6, 14). The tertiary structure of MccJ25 was elucidated as a lariat peptide (1, 10, 17). It contains an eight-residue ring (G1 to E8) and a tail (Y9 to G21) whose C-terminal end is sterically trapped within the ring. MccJ25 amino acids F10 to P16 form a β-hairpin structure, comprising two β-strands (F10-V11 and T15-P16) and a β-turn (V11 to G14).
MccJ25 is active on gram-negative bacteria related to the producer strain, and among them are several human pathogens (11, 12, 16). It was previously shown that the E. coli RNA polymerase (5, 18) and the bacterial respiratory chain (2, 9) are the targets for MccJ25 action. MccJ25 is active on pathogenic strains of Salmonella spp., Shigella spp. (12), and E. coli, including O157:H7 (11) as well as non-O157 strains (data not shown), that frequently cause outbreaks of food-borne diseases. In addition, Sable et al. (11) showed that MccJ25 was the most active microcin against 12 out of 15 diarrheagenic E. coli strains tested. These authors also demonstrated that MccJ25 inhibits E. coli O157:H7 in biological products such as milk, egg yolk, and meat extract. These findings suggest that MccJ25 could be an efficient complement to nisin for food preservation. However, the potential usefulness of MccJ25 is compromised by the fact that it is highly resistant to digestion by proteolytic enzymes of the stomach (pepsin) and intestinal (trypsin, chymotrypsin, and carboxypeptidases) contents. Thus, the antibiotic would most likely remain active in the intestine, and this could lead to disturbance of the normal microbiota. Therefore, for potential use of MccJ25 as a food additive, it would be desirable to render MccJ25 susceptible to at least one of these proteases. In the present work, we describe a chymotrypsin-susceptible MccJ25 derivative that remains fully active on S. Newport and E. coli O157:H7 in biological products, namely milk and egg yolk. In addition, we demonstrate that the peptide is inactivated by rat intestinal contents.
Selection of MccJ25 derivatives susceptible to chymotrypsin digestion.
Recently, a complete collection of mutants with point substitutions in the MccJ25-encoding part of mcjA was constructed and characterized (8). Despite the complex three-dimensional structure and multistep processing/maturation of MccJ25, a surprisingly large proportion of precursor McjA polypeptide derivatives were found to mature, and most microcin variants retained their antibacterial function. In particular, it was found that substitutions in the β-turn and surrounding amino acids were well tolerated. Since chymotrypsin is selective for peptide bonds with aromatic or large hydrophobic side chains on the carboxyl side, we selected for further analysis the functional MccJ25 derivatives with the following mutations in this region: I13W, T15F, G12Y, and T15W. Previous work indicates that cleavage of the polypeptide backbone of MccJ25 in the β-turn region abolishes its antibacterial activity (3, 13), so we expected that if chymotrypsin-sensitive microcin derivatives were found, they would be inactivated by proteolytic digestion. To determine the sensitivity of MccJ25 derivatives to chymotrypsin, 10 μl of cultured medium of cultures of cells producing wild-type or substitution-containing microcins was spotted onto M9 plates and 10 μl of 0.5 mg/ml chymotrypsin solution was placed 1 cm away from the cultured medium drops. After the drops had dried, the plates were overlaid with 4 ml of soft agar inoculated with 107 cells of a clinical isolate of Salmonella enterica serovar Newport (16). After overnight incubation at 37°C, the growth inhibition zones around the drops of MccJ25-containing media were recorded (Fig. 1). Circular growth inhibition zones were observed around drops containing wild-type MccJ25 as well as drops containing MccJ25(T15F) or MccJ25(T15W). In contrast, growth inhibition zones around drops containing MccJ25(G12Y) and MccJ25(I13W) peptides contained a crescent-shaped distortion at the side closest to the chymotrypsin drop. We interpret the shapes of growth inhibition zones around these derivatives as suggesting that chymotrypsin that diffuses out of its spot digests and detoxifies them, decreasing the efficiency of indicator strain growth inhibition. The other two MccJ25 derivatives were apparently not affected by chymotrypsin.
Antimicrobial activity of MccJ25 derivatives.
Sensitivity to purified microcins was tested by a spot-on-lawn assay as follows. To avoid aggregation (4), concentrated methanolic solutions of wild-type or variant microcins (2 mM) were prepared and serial doubling dilutions in double-distilled water containing 0.1% Tween 80 were made. Aliquots (10 μl) of each dilution were spotted onto M9 medium plates supplemented with 0.2% tryptone. After the drops had dried, the plates were overlaid with 4 ml of soft agar (0.6%) containing the indicator strain. The plates were incubated for 12 h at 37°C before examination. The concentration (μM) of the last dilution giving a clear or turbid-growth inhibition zone was defined as the MIC.
Sensitivities of the S. Newport and E. coli O157:H7 (clinical isolate) strains to native MccJ25, MccJ25(G12Y), and MccJ25(I13W) were compared and are reported in Table 1. In agreement with previous data, the MccJ25(G12Y) peptide displayed antibiotic activity similar to that of the native MccJ25. This microcin derivative was also most susceptible to chymotrypsin digestion (Fig. 1). For this reason, MccJ25(G12Y) was selected for the rest of the experiments.
MccJ25(G12Y) cleavage by digestive enzymes.
Experiments described hereafter were performed on female albino rats of a locally bred strain (originally Sprague-Dawley stock). Per the guidelines of the American Physiological Society and as approved by the animal board of the Consejo Nacional de Investigaciones Científicas y Técnicas, only rats weighing 220 to 250 g were used in this study.
To obtain intestinal contents, the rats were anesthetized with ketamine (0.3 mg/kg of body weight) and xylazine (Rompun) (16 mg/kg of body weight). A midline abdominal incision was made, and the end of the small intestine was identified and ligated to a polyethylene catheter through which the intestinal efflux was collected. The sensitivity of MccJ25(G12Y) to digestive enzymes was assayed by incubating 5 μl of the peptide solution (1 mg/ml) in 20 mM phosphate buffer, pH 7.0, with 45 μl of the intestinal contents at 37°C for 24 h. As a control, an aliquot of intestinal contents was heated in boiling water for 10 min (to inactivate the digestive enzymes) and also incubated with MccJ25(G12Y). All samples were assayed for residual antimicrobial activity as described in "Antimicrobial activity of MccJ25 derivatives" (above). MccJ25(G12Y) lost its inhibitory activity after incubation with intestinal contents (Fig. 2B), whereas native MccJ25 remained fully active (Fig. 2D). No effect was observed when both peptides were incubated with boiled intestinal contents (Fig. 2A and C). The most likely explanation for these results is that loss of activity of MccJ25(G12Y) was caused by a digestive enzyme.
In vivo inactivation of MccJ25(G12Y) by digestive enzymes.
For the following experiments, rats were housed in three group cages (three animals per cage). Two groups of animals were orally given 0.2 ml of a solution of MccJ25 or MccJ25(G12Y) (both at 0.5 mg/ml), and a third group was inoculated with the same volume of sterile water as a control. Efflux from the intestine was recovered as described above. The collection of intestinal contents began immediately after administration of microcin and continued for 60 min. Intestinal samples were then subjected to antimicrobial activity detection. The original antimicrobial activities of the two peptide solutions used in the assay were also determined.
Figure 3A and B show that considerable native MccJ25 activity was recovered after MccJ25 passage through the intestinal lumen. The observed decrease in activity could, at least in part, be explained by the dilution with intestinal fluid. On the contrary, no MccJ25(G12Y) activity appeared after passage of that protein through the intestine (Fig. 3C and D). In this case, the possibility of a dilution effect could be discarded, since when the experiment was repeated with a 10-fold-concentrated sample, no activity was detected (data not shown). Samples collected from the control group (inoculated with water) did not show any antimicrobial activity.
Antimicrobial activity in food products.
To evaluate the antimicrobial activity of MccJ25(G12Y) in biological products, 1 ml of sterile skim milk (10%, wt/vol) and diluted egg yolk (1:5 mixture of sterile egg yolk and sterile distilled water) were inoculated with about 104 cells/ml of either S. Newport or E. coli O157:H7, in the presence or absence of MccJ25(G12Y) (1 μg/ml), and incubated at 37°C. At various times, aliquots of both food samples were withdrawn and viable bacterial counts were determined. The MccJ25(G12Y) peptide efficiently inhibited the growth of both pathogenic strains in egg yolk and milk (Fig. 4A and B, respectively). This result suggests that the antibiotic can exert its activity in food samples and demonstrates its stability in biological products.
The thermostability of MccJ25(G12Y) was also assessed. To this end, a sample of peptide in phosphate buffer (1 mg/ml) was autoclaved (121°C for 20 min) and then cooled and tested for antimicrobial activity. The microcin derivative showed high heat resistance similar to that of wild-type microcin (data not shown). This property may allow the use of this peptide in processes requiring high temperature.
As far as we know, this study represents the first example of a rational modification of any microcin with the aim of a potential use in food preservation. This strategy could open the way to attempting similar modifications in other peptide antibiotics which are naturally resistant to digestive enzymes.
This work was funded by grants PICT 2107 and PICTO 843 from the Agencia Nacional de Promoción Científica y Tecnológica and R03 grant TW006828 from the NIH FIRCA. M.F.P. was a recipient of a fellowship from CONICET. R.A.S., R.F., and P.A.V. are Career Investigators from CONICET. Work in the laboratory of K.S. was supported by NIH grant GM64530.
- Received 11 May 2009.
- Accepted 3 July 2009.
- ↵*Corresponding author. Mailing address: Departamento de Bioquímica de la Nutrición, INSIBIO, Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina. Phone: (54) (381) 4248921. Fax: (54) (381) 4248921. E-mail: pvincent{at}fbqf.unt.edu.ar
↵▿ Published ahead of print on 10 July 2009.
- American Society for Microbiology


text:
The Old Religion.New York : 1959 Stock Code: 51514
NotesFirst edition, first printing. From the library of the author.
Octavo. Original grey wrappers printed in black.
Wrappers rubbed and marked, contents a little toned and warped. A good copy.
With the exception of framed items*, Peter Harrington offers free delivery on all UK orders of rare books, maps and prints placed through this website. Delivery to USA and the rest of the world is similarly free for orders over £200.
Established in 1969, Peter Harrington is one of the leading rare book firms in the world. It is a proud member of the Antiquarian Booksellers Association – along with ILAB, the PBFA and Lapada – and from shops in Mayfair and Chelsea, London, sells rare books, prints and ephemera to customers across the world.
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One of the most popular theories around Coronavirus is it being a Chinese Bio-Weapon. However, a research paper titled The proximal origin of SARS-CoV-2 puts end to such theories, claiming that the theory has no scientific ground.Read More
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CLAIM TO FAME: A protein-rich pasta made out of premature green soybeans
FAST FACTS: Eaten as a protein-rich snack in Japan, Korea, and Hawaii; made by using traditional Asian methods; has the same consistency as regular pasta; high-quality green beans are used instead of wheat or starch; gluten-free, certified organic, and verified non-GMO
PERFECT FOR: Protein-rich pastas
WHAT YOU GET: (1) pack Organic Edamame Protein Pasta
NET WEIGHT: 200g
EXPIRY: 1 year
INGREDIENTS: Organic Green Soybeans (Edamame), Water
Cook until al dente. Store at room temperature.
7Grains Company aims to offer convenient and sustainable food substitutions for you and your family. They source and create the best possible healthy alternatives to food that we normally consume at wallet-friendly prices. Their products are low in calories and contain significantly less sugar and salt than its commercial counterparts. They are adamant about not using artificial colors and flavor enhancers as well as all other bad-for-you ingredients—only honest ingredients that are abundant with nutritional benefits. Much of their efforts are placed on verifying, researching, and collaborating with their in-house food technicians and third-party organizations, locally and internationally, to ensure the safety and integrity of all their products. Make healthier choices with the 7Grains Company.
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First time I was able to eat protein pasta, it has rougher texture than the usual pasta but it is more filling and has a nutty taste since it's plant- based. One serving (200g) is good for a family of 6-8. I read in one of the comments that this is better when using Asian dressing, so I made pasta in oil with dulong and mushroom.24 days ago
Wow! I did not think this would taste good but wow! It did! It worked well even with spaghetti sauce! The whole box was finished in an instant. It had a noodly and light earthy smell but it do taste good! It's a bit rubbery though but add some oil on it and that will do the trick. I have yet to try it on pesto but I'm sure it will work well with pesto! This is a great alternative if you are avoiding normal pasta with high carbs. I just hope there is more inside one box! Would I buy this again? Definitely yes!3 months ago
I saw this pasta when I was scrolling to find good product to include in my diet. And wow I ordered this right after I saw it because I am planned to have a healthy pasta. Being in a diet doesn't mean you can't have delicious food. I cooked this with pesto and tuna in brine with lots of garlic. You can use olive oil since it works for me. Sobrang naenjoy ko ang ng mom ko. For me, nadivide ko sya into 6 servings since diet nga ako. Love it!❤4 months ago
I have decided to try this protein based pasta out of curiosity and I am glad I was able to get my hands on it just one day after ordering it(many thanks beautymnl!) It tasted really good! You wouldn't even notice that it is purely made out of beans! It is definitely a healthier alternative, rich in protein pasta as compared to the usually over processed ones.6 months ago
But it's not practical to use this all the time or everytime you need to eat pasta. Though the health benefits that's been said in the labels is true, still not practical to use this always or as often as you would want to. I was just being curious when i purchased this. GBU seller and BMNL.7 months ago
As someone who's cutting down on carbs and prioritizes protein intake, this is a solid addition to my prepped meals. However, a word of caution -- don't expect this to become a lot thicker than when it's dry. The 50g serving may not be filling enough on its own so you might want to add toppings to your heart's content. Gonna repurchase (planning on stocking up actually).8 months ago
The BeautyMNL Awards: The 33 Best Food & Drink Products of 2019
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Under P300: 7 Food Swaps That Make Getting Healthy Easy
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Great gifts that are good for them.


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Table Contents
In some cases, there are no symptoms — this happens at times with type 2 . In this case, people can live for months, even years without knowing they have the disease. This form of comes on so gradually that symptoms may not even be recognized.
develops gradually, but the symptoms may seem to come on suddenly. When there's no more insulin in the body, blood glucose levels rise quickly, and these symptoms can rapidly develop: Extreme weakness and/or tiredness.
If type 2 goes untreated, the high blood sugar can affect various cells and organs in the body. Complications include kidney damage, often leading to dialysis, eye damage, which could result in blindness, or an increased risk for heart disease or stroke.
No matter how thin you are, you can still get . "Diabetes comes from insulin resistance, which causes high blood sugar. While about 80 percent of people with are overweight or obese, it happens to thin people as well." Right now, 30 million people in the U.S. have .
Diabetes can be effectively managed when caught early. However, when left untreated, it can lead to potential complications that include heart disease, stroke, kidney damage, and nerve damage. If you have , your pancreas either produces too little insulin or none at all.
According to a 1995 CNN.com article, with , excess blood sugar, or glucose, in your body draws water from your tissues, making you feel dehydrated. To quench your thirst, you drink a lot of water and other beverages which leads to more frequent urination.
You have multiple signs and symptoms of diabetic ketoacidosis — excessive thirst, frequent urination, nausea and vomiting, abdominal pain, shortness of breath, fruity-scented breath, confusion.
You could have and not know it. The symptoms of can be very mild. In fact, of the 29 million people in the U.S. who have , 8 million are undiagnosed, according to the American Diabetes Association. However, you don't know just by your symptoms if you have .
Sugar doesn't cause . But there is one way that sugar can influence whether a person gets type 2 . Consuming too much sugar (or sugary foods and drinks) can make people put on weight. ... Weight gain from eating too much of any food can make a person's chance of getting greater.
Diabetes and nocturia. Having high blood glucose levels can cause the body to excrete excess glucose via the urine. ... If you regularly have high blood glucose levels, you may increase the risk of picking up a urinary tract infection which can also increase the need to urinate through the night.
on the internet has become a popular way to promote a business. Nonetheless, in order to become successful, you've got to learn the methods to approach it. Just because you've got a web site, it does not imply that your company will automatically become successful just because you've got a web site. Read these tips for some advice on the best way best to become an effective online marketer|internet marketer that is effective}.
Spend some time getting an authority on your industry. Write a website, visit with see other related blogs and leave comments, answer queries to help websites and find forums which you can join and take part in. Any time you can place your name, your company's name and your experience out there, it is going to enhance your standing on the web as an expert in your area reputation.
Remember the point of your website needs to be to entice clients. If your website is bogged down with excessive information, your clients will get lost Your customers will inevitably get lost if your website is bogged down with excessive information, if your site is bogged down with excess data and you will lose hundreds of potential buyers. Never forget that there's a time for information, and a time for language that is attractive.
Create a blog that's directly linked with your website. This raises awareness of your brand, and it is a different, creative different avenue to link to articles on your website. A blog with {your company's the name of your company implies you automatically have a search outcome that relates to a business have. Eventually, they can join a blog community and contribute intelligent give opinions to other sites, which increases internet exposure comments.
Monitor your information. Locate software which does the monitoring for you, and use it in order to figure what that happens with your site out. Track your sales, site hits new clients, and the background of your obligations. {Doing so can make certain you have the documents necessary to back yourself up|This can ensure you have the records required to back up yourself doing|This can make certain you have the documents required to back up yourself, doing So can ensure should problems ever arise with your contracted company.
Consider blog or starting a podcast. A good podcast will keep clients coming back to check out the new content. Having a podcast, you allow people to multitask while hearing about your products or testimonials. {This may translate into earnings in the event that you're able to keep them returning If you can keep them returning, this may translate into sales.
Post frequently on your site and change the design as frequently as possible. The look will get mundane after a while and might detract visitors from viewing your website. {The more you post, the more you'll seem like you are involved with your business and the products that you provide.
Whenever you are communicating with the customers on your site, attempt to be unique and interesting. If you encounter as a robot with no character, your customers will be attracted away, if you encounter as a robot with no personality|Your customers will be attracted away if you come across as a robot with no personality. Creating a personality on your site, will help establish a much stronger personal connection with your potential customers, yielding more earnings.
If you are creating an email list to advertise your online product or business, be sure to use branding. Your emails should definitely be connected {with your company, through the use of colour, logos, and writing style. This helps your clients feel that your organization has a personal connection with them.
Designing a website to advertise your company online is an exciting yet difficult process, particularly with all of the video and audio aids which you can use to enhance it. But don't fall prey to the frequent mistake made by beginners of thinking that more is better. Even a Flash presentation takes a very long time to load and many consumers would move on to another firm's site of the next company than waste their time waiting. Pop-ups are not anything more than an annoyance to many buyers. Restrict your use of photographs and images, and be certain they are all relevant. {If you decide to add music to your site, give the user the choice of clicking on a button to hear, if you decide to add music to your site. A customer who's currently visiting with your store while at work or a person who is in the home with a sleeping baby in earshot, is not going to love music or sales pitches that play automatically.
To help your business grow with online , think about hosting a giveaway. Everybody likes winning, and no one will turn down a free gift! Giveaways will bring visitors to your site, and people will look around to learn about your goods and services before entering to win. The delight of a giveaway will produce a fun buzz around your business.
Going the route that is email in online can really well backfire on you and in a hurry. Make sure that any mail you're sending that is short, informative, and introduces a call to action to the potential customer. The idea is for people to read your message and feel the urge to click, not to read a lengthy story and click out of your message, out of boredom.
A fantastic method of concerning online is to add tricky words that are catchy {to your advertising. Using words such as fast and focusing on phrases like quickly ordering or fast results will encourage the consumer to use the product which you are selling. A good illustration is to state things such as Our product works fast.
If you're going to use a movie or an image make sure that you label it properly. People using Google Images or some similar some search engine will find your picture and end up on your website if they click on it. It works the identical manner with videos.
Write a response article. In case you've seen other articles voicing opinions that you do not agree with, compose your own article that displays your perspectives. Be polite and use logical arguments. People generally love to see arguments, so if you link up to the article, your audience will skyrocket.
{There are lots of aspects to internet that can either make or break an online business. You need to understand what works for your company and what does not. The only way is by trying different procedures and monitoring the outcomes. Continue reading to learn by reading articles and talking to experts. If you work hard in {your internet your business, you can reap the benefits of your labor.


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