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- Price$289,000
- Beds3
- Baths2
- SQ. Feet1,329
- Acres0.19
- Built1999
1104 Buffalo Gap Rd, Austin
Perfect house for first time Home Buyer or Investor! NO HOA! DEEDED ACCESS TO LAKE TRAVIS! LAKE TRAVIS ISD! QUIET PEACEFUL NEIGHBORHOOD WITH ALL THE AMENITIES OF LAKEWAY WITHOUT THE RESTRICTIONS! SHORT TERM RENTALS ALLOWED. 3/2 open layout All Sides Brick Vaulted ceilings in the living room! Kitchen opens to Living Room! Lots of Live oak Tress both in the front and backyard! No Carpet! Tile in Living and Kitchen area! Seller is offering a $1500 flooring allowance for 2 bedrooms! Carpet has been removed!
Essential Information
- Price$289,000
- Bedrooms3
- Bathrooms2.00
- Full Baths2
- Square Footage1,329
- Acres0.19
- Year Built1999
- TypeResidential
- Sub-TypeHouse
- Style1st Floor Entry
- StatusActive
- UtilitiesElectricity on Property, Propane
- ParkingGarage, Attached, Entry-Front
- # of Garages1
- ViewNo View
- Exterior FeaturesPatio-Covered, Private BackYard, Storage Building
- Lot DescriptionLevel
- RoofComposition Shingle
- ConstructionAll Sides Masonry, Composition Shingle
- FoundationSlab
Community Information
- Address1104 Buffalo Gap Rd
- SubdivisionStewart B.K.
- CityAustin
- CountyTravis
- StateTX
- Zip Code78734
- Interior FeaturesCeiling-High, Ceiling-Vaulted, Walk-In Closet, Skylight, Smoke Detector
- AppliancesCook Top Gas, Disposal, Dishwasher, Water Heater Gas, Microwave Oven, Refrigerator
- HeatingCentral Heat
- CoolingCentral Air
- # of Stories1
- Stories1
School Information
- DistrictLake Travis ISD
- ElementaryLake Travis
- MiddleHudson Bend
- HighLake Travis
Price Change History for 1104 Buffalo Gap Rd, Austin, TX (MLS #1470689)
|Price Reduced from $294,000 to $289,000
|Price Reduced from $299,000 to $294,000
Property Listed by: Austin Capitol Realty
The information being provided is for consumers' personal, non-commercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing.
Based on information from the Austin Board of REALTORS® (alternatively, from ACTRIS) from April 8th, 2020 at 1:45pm CDT. Neither the Board nor ACTRIS guarantees or is in any way responsible for its accuracy. The Austin Board of REALTORS®, ACTRIS and their affiliates provide the MLS and all content therein "AS IS" and without any warranty, express or implied. Data maintained by the Board or ACTRIS may not reflect all real estate activity in the market.
All information provided is deemed reliable but is not guaranteed and should be independently verified.


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Each year, thousands of like-minded people come together for the largest gathering of afterschool professionals and leaders.
To outdo yourself generally means to do something exceptional or superb—especially in relation to past efforts.
As a new school year approaches, planning ahead financially is usually in the front of every afterschool leader's mind—though finding helpful resources can sometimes be a challenge.
A few weeks ago, I wrote about my visit to our NAA storage facility to go through years and years of organizational files. With NAA's 30th anniversary coming up in 2018, I find myself spending more and more time thinking about how to honor the past of our work, while also looking toward the future.
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Product Specification
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Product Details
- HP Officejet Pro 9020 All-in-One - Multifunction printer - colour - ink-jet - Legal (216 x 356 mm) (original) - A4/Legal (media) - up to 39 ppm (copying) - up to 39 ppm (printing) - 250 sheets - USB 2.0, LAN, Wi-Fi(n), USB host
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|Device Type
|Multifunction printer
|Fax Type
|Plain paper
|Printing Technology
|Ink-jet - colour
|Monthly Duty Cycle (max)
|30000 pages
|Recommended Monthly Volume
|2000 pages
|Display Diagonal Size
|Colour Support
|Display Features
|Touch screen
|Connection Availability
|USB 2.0, LAN, Wi-Fi(n), USB host
|AirPrint Enabled
|Office Machine Features
|Print from USB flash drive, scan to e-mail, Mopria certified, HP Instant Ink
|EMEA (excluding Israel, Saudi Arabia, South Africa)
|Standard Memory
|Max Supported Memory
|Max Copying Speed
|Up to 39 ppm (mono) / up to 39 ppm (colour)
|Max Copying Resolution
|Up to 600 dpi (mono) / up to 600 dpi (colour)
|Max Document Enlargement
|Max Document Reduction
|Automatic Duplexing
|Copying Features
|ID Card Copy, collation copy
|Inkjet Technology
|HP Thermal Inkjet
|Max Printing Resolution
|Up to 1200 x 1200 dpi (mono) / up to 4800 x 1200 dpi (colour)
|Max Printing Speed
|Up to 39 ppm (mono) / up to 39 ppm (colour)
|Ink Cartridge Configuration
|4 cartridges (1 each: cyan, magenta, yellow, black)
|Ink Palette Supported (Colours)
|Cyan, magenta, yellow, black
|Printer Drivers / Emulations
|Automatic Duplexing
|First Print Out Time B/W
|9 sec
|First Print Out Time Colour
|10 sec
|Scan Element
|Automatic Duplexing
|Optical Resolution
|1200 x 1200 dpi
|Grayscale Depth
|Colour Depth
|Fax Machine
|Fax Resolutions
|203 x 98 dpi, 203 x 196 dpi, 300 x 300 dpi, 200 x 200 dpi
|Total Memory Capacity
|Up to 100 pages
|Fax Printing Resolution
|203 x 196 dpi
|PC Faxing
|Fax Machine Features
|Speed Dialing
|99 station(s)
|Delayed Transmission
|Fax forwarding, automatic redialing, colour faxing, junk fax barrier, auto reduction, Distinctive Ring Detection (DRD), delayed sending
|Document & Media Handling
|Min Original Size
|76.2 x 127 mm
|Max Original Size
|Legal (216 x 356 mm)
|Original Type
|Document Feeder Capacity
|35 sheets
|Min Media Size
|76.2 x 127 mm
|Max Media Size
|Min Media Weight
|60 g/m²
|Max Media Weight
|280 g/m²
|Min Media Weight (lb)
|16 lbs
|Max Media Weight (lb)
|110 lbs
|Supported Media Type
|Plain paper, photo paper, thick paper, card stock, glossy paper, matte paper
|Supported Media Sizes
|ANSI A (Letter) (216 x 279 mm), Legal (216 x 356 mm), Executive (184 x 267 mm), A4 (210 x 297 mm), A5 (148 x 210 mm), A6 (105 x 148 mm), 100 x 150 mm, 130 x 180 mm, JIS B5 (182 x 257 mm), 101.6 x 152.4 mm, Statement (139.7 x 215.9 mm), 76.2 x 127 mm, 127 x 177.8 mm, 203.2 x 254 mm
|Supported Envelope Sizes
|US No 10 (104.7 x 241.3 mm), Monarch (98.4 x 190.5 mm)
|Borderless Photo Sizes
|ANSI A (Letter) (216 x 279 mm)
|Standard Media Capacity
|250 sheets
|Max Media Capacity
|250 sheets
|Output Trays Capacity
|100 sheets
|Document & Media Handling Details
|ADF - 35 sheets - Legal (216 x 356 mm) ¦ Input tray - 250 sheets - Legal (216 x 356 mm) weight: 60 g/m² - 280 g/m² ¦ Output tray - 100 sheets
|Speed Details
|Printing: up to 24 ppm (Letter A) - B/W (ISO) ¦ Printing: up to 24 ppm (Letter A) - colour (ISO) ¦ Printing: up to 39 ppm (Letter A) - B/W draft ¦ Printing: up to 39 ppm (Letter A) - colour draft ¦ Copying: up to 39 ppm (Letter A) - B/W ¦ Copying: up to 39 ppm (Letter A) - colour ¦ Scanning: up to 15 ipm - B/W ¦ Scanning: up to 8 ipm - colour
|2 x modem - RJ-11 ¦ 1 x LAN - RJ-45 ¦ 1 x USB 2.0 - 4 pin USB Type B ¦ 1 x USB host - 4 pin USB Type A
|Operating System Support
|Apple Mac OS X 10.8, Apple Mac OS X 10.9, MS Windows 7 (32/64 bits), Apple Mac OS X 10.10, MS Windows XP 32-bit Edition SP3 or later, Windows 8 (32/64-bits), Windows 8.1 (32/64-bits), Windows 10 (32/64-bits), MS Windows Vista 32-bit Edition
|Software Included
|Software HP Printer
|Voltage Required
|AC 120/230 V (50/60Hz)
|Power Consumption Standby
|5.8 Watt
|Power Consumption Sleep
|1.22 Watt
|Environmental Standards
|ENERGY STAR Certified
|Manufacturer Warranty
|Service & Support
|Limited warranty - 1 year
|Dimensions & Weight
|43.7 cm
|39.63 cm
|31.83 cm
|12.02 kg
|Dimensions & Weight (Shipping)
|Shipping Width
|49.6 cm
|Shipping Depth
|37.7 cm
|Shipping Height
|48.9 cm
|Shipping Weight
|15.06 kg
|Environmental Parameters
|Sound Emission
|53.5 dBA (operating)
|Min Operating Temperature
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|Humidity Range Operating
|25 - 75%
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Key View:
Over the long term our view on Turkey's banking and financial services market is positive based on the market being wellregulated, stable and competitive, and with the local capital market boasting relatively high liquidity. We note that there are plenty ofnear-term headwinds facing the four markets we track, with downside risk growing within each. We point to the lira remainingvulnerable to market jitters and policymakers risking exacerbating imbalances and pushing the economy onto a unstable growthpath as key factors shaping this view. In the banking sector conditions are becoming increasingly challenging, and we expect to seethe sector continuing to deleverage as financing conditions remain unfavourable. This has seen the government becomeincreasingly proactive in order to address the sector's fragile funding structure by speeding up the recovery in public banks' balancesheets. A significant development this quarter has seen Turkey's plan to establish its own credit ratings agency realised through theacquisition of JCR Eurasia. We see the larger non-life market continuing to enjoy far greater premiums growth in the insurance arena,the asset management industry remains largely underdeveloped and the Borsa İstanbul exchange is continuing to struggle inattracting new listings.
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Ultra Music Festival is returning to its home in Bayfront Park in Miami, FL, USA, which is also set to be completely refurbished, as announced recently. Resistance, which is the Techno brand of Ultra with events across the world, has grown massively since its inception. It
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Selective desulfurization of dibenzothiophene by newly isolated Corynebacterium sp. strain P32C1
Selective desulfurization of dibenzothiophene by newly isolated Corynebacterium sp. strain P32C1
Selective desulfurization of dibenzothiophene by newly isolated Corynebacterium sp. strain P32C1
Biochemical Engineering Journal 5 (2000) 11±16 Selective desulfurization of dibenzothiophene by newly isolated Corynebacterium sp. strain P32C1 S. Ma...
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Biochemical Engineering Journal 5 (2000) 11±16
Selective desulfurization of dibenzothiophene by newly isolated Corynebacterium sp. strain P32C1 S. Maghsoudia, A. Kheirolomooma,*, M. Vossoughib, E. Tanakac, S. Katohd
a Department of Chemical Engineering, Sharif University of Technology, Tehran, Iran Biochemical & Bioenvironmental Research Center, Sharif University of Technology, Tehran, Iran c Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan d Graduate School of Science and Technology, Kobe University Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan b
Received 10 September 1999; accepted 15 October 1999
Abstract Corynebacterium sp. strain P32C1 was selected from 30 strains isolated from soil samples of various areas in Iran on the basis of the ability to utilize dibenzothiophene (DBT) as a sole source of sulfur. During 27 h of cultivation in a jar-fermentor, DBT with an initial concentration of 0.25 mM was completely converted to 2-hydroxybip henyl (2HBP). Concentration change of 20 -hydroxybiphenyl-2sul®nate (HBPS) during cultivation showed the general characteristics of reactions in series, DBT ! HBPS ! 2HBP. With resting cells prepared in late exponential phase, DBT of 0.5 mM was completely converted to 2HBP during 30 min, and the maximum speci®c production rate of 2HBP was 37 mmol/(kg dry cells h). Fed-batch addition of DBT in the jar-fermentor resulted in growth continuing up to a high 2HBP concentration of 0.4 mM. Desulfurization ability of P32C1 strain was compared with Rhodococcus sp. IGTS8 as a standard strain, and it was thought that P32C1 strain had a higher desulfurization activity. # 2000 Elsevier Science S.A. All rights reserved. Keywords: Biodesulfurization; Dibenzothiophene; Rhodocuccus sp. IGTS8; Corynebacterium sp. P32C1
1. Introduction Sulfur-containing compounds in fossil fuels represent major problems for the petroleum and coal industries. Without treatment at the re®ning and production stages or elimination of noxious gases on combustion, the use of oil and coal fuels results in polluting or corrosive sulfurcontaining emissions and the acid rain. Governments throughout the world have recognized the problems associated with these emissions and moved to reduce them through legislations [1,2]. The conventional method for desulfurization of middle distillates like kerosene and diesel is hydrodesulfurization (HDS). Hydrodesulfurization is operated under high-pressure (150±3000 lb/in2) and high temperature (290±4558C) and uses hydrogen gas in presence of metal catalysts to reduce the sulfur in petroleum fractions to hydrogen sul®de [3]. Investment and operating costs of this process are high and, in addition, it does not work
* Corresponding author. Tel.: 98-21-600-5417; Fax: 98-21-601-2983. E-mail address:
[email protected]
(A. Kheirolomoom).
well on certain sulfur compounds like polycyclic sulfur compounds. Biocatalytic desulfurization (BDS) of petroleum fractions offers an attractive alternative to HDS process. For this process to be commercial, microorganisms with high activity and selectivity are required. Dibenzothiophene (DBT) was widely used as a model sulfur compound for isolation and enrichment of suitable strains. Several microorganisms were found to desulfurize DBT through a selective pathway to 2-hydroxybiphenyl (2HBP). In this pathway (4S pathway) shown in Fig. 1, carbon bonds remain intact and the heating value of fuels remains constant. Rhodococcus sp. IGTS8 (ATCC 53968) [4,5], Corynebacterium SY-1 [6], Rhodococcus erythropolis D-1 [7], a thermophilic strain of Paenibacillus [8], and Gordona strain CYKS1 [9] were observed to selectively desulfurize DBT to 2HBP. For Rhodococcus sp. IGTS8 molecular cloning and characterization of genes responsible for sulfur oxidation have been studied [10,11,12], and the enzymatic system for desulfurization pathway has been elucidated [13,14,15,16]. In this work, growth of newly isolated strain P32C1 and biodesulfurization of DBT by growing and resting cells were
1369-703X/00/$ ± see front matter # 2000 Elsevier Science S.A. All rights reserved. PII: S 1 3 6 9 - 7 0 3 X ( 9 9 ) 0 0 0 5 5 - 8
S. Maghsoudi et al. / Biochemical Engineering Journal 5 (2000) 11±16
2.2. Bacterial strains Rhodococcus erythropolis IGTS8 (ATCC 53968), which formerly was identi®ed as Rhodococcus rhodochrous was used as a standard and well-characterized strain for comparison and control. Corynebacterium sp. P32C1 was isolated from 40 soil samples from different coal mining areas and oil production ®elds in Iran. One gram soil of the samples was suspended in 10 ml of a saline solution, and after standing for 1 h, 2 ml of the supernatants was transferred to 20 ml sulfur-free Medium A [7] supplemented with 5.4 mM DBT as sulfur source and 5 g/l glucose (Table 1). During one week cultivation, single-colony isolation was repeated on the plate of the same medium containing 1.5% agar, and ®nally, isolated colonies were streaked on the plate of Tryptic Soy Agar (TSA, Difco). Selection of P32C1 strain among 30 isolated strains was based on ability to grow in the presence of DBT and production of 2HBP from DBT. 2.3. Media and growth conditions Fig. 1. Selective pathway (4S pathway) for DBT desulfurization without cleavage of carbon±carbon bonds.
studied and its characteristics were compared with those of Rhodococcus sp. IGTS8 as a standard strain. 2. Materials and methods 2.1. Chemicals DBT (Nacalai Tesque, Kyoto, Japan), Dibenzothiophene sulfone (Aldrich, Milwaukee, Wis., USA), 2HBP (Wako, Osaka, Japan), and 2,20 -dihydroxybiphenyl (Tokyo Kasei Kogyo, Tokyo, Japan) were used. 20 -Hydroxybiphenyl-2sul®nate (HBPS) in the form of sultine (dibenz[c,e][1,2]oxathiin-6-oxide) was synthesized by the technique of Hanson and Kemp [17]. All other chemicals were of analytical grade, commercially available and used without further puri®cation.
Sulfur-free Medium A [7] containing 5 g/l glucose and supplemented with 5.4 mM DBT was used for selective enrichment and isolation of new strains. For growth study of P32C1 in jar-fermentor, glucose was replaced by the same amount of glycerol in Medium A, and DBT concentration was reduced to 0.25 mM. Growth media for IGTS8 strain was BSM medium [4] containing 20 mM glycerol and DBT of 0.25 mM (Table 1). Depending on the volume of the medium used, two different DBT±ethanol solutions of 27 and 100 mM were prepared and added to sterilized medium. For subculture and small-scale experiments 500 ml ¯asks or test tubes (18 180 mm) were shaken at 308C and 150 rpm. The cell concentration was determined from the optical density at 660 nm (OD660). 2.4. Culture in jar-fermentor Batch and fed-batch culture of strains have been performed in a jar-fermentor (MDS-U50, B.E. Marubishi, Tokyo, Japan) with working volume of 1.0 l. The tempera-
Table 1 Composition of media (without carbon source) Medium A [7]
BSM medium [4]
Medium A Deionized water (l) KH2PO4 (g) K2HPO4 (g) NH4Cl (g) MgCl26H2O (g) CaCl2 (g) NaCl (g) Metal solution (ml) Vitamin mixture (ml)
Metal solution 1 0.5 4 1 0.2 0.02 0.01 10 1
FeCl24H2O (g) ZnCl2 (g) MnCl24H2O (g) Na2MoO42H2O (g) CuCl2 (g) Na2WO42H2O (g) HCl (mmol) Deoinized water (l)
Vitamin mixture 0.5 0.5 0.5 0.1 0.05 0.05 120 1
Calcium pantothenate (mg) Inositol (mg) Niacin (mg) Pyridoxine hydrochloride (mg) P-Aminobenzoic acid (mg) Cyanocobalamin (mg) Deoinized water (l)
400 200 400 400 200 0.5 1
Deoinized water (l) KH2PO4 (g) Na2HPO4 (g) NH4Cl (g) MgCl26H2O (g) CaCl22H2O (g) FeCl36H2O (g)
1 2.44 5.57 2 0.2 0.001 0.001
S. Maghsoudi et al. / Biochemical Engineering Journal 5 (2000) 11±16
ture was kept at 308C and the dissolved oxygen concentration in the culture medium was controlled to be not lower than 50% of oxygen saturation concentration by changing the aeration rate, the ratio of pure oxygen to air and/or agitation speed. Antifoam agent (KM-70, Shin-Etsu Chemical, Tokyo) was used to suppress the foaming. 2.5. Reaction with resting cells Both strains were grown in the jar-fermentor containing Medium A with 0.25 mM DBT. Cells were harvested during growth or in the late logarithmic phase by centrifugation at 10 000 g for 10 min at 48C, washed twice with 0.1 M potassium phosphate buffer (pH 7.0). The harvested cells were directly used or stored at ÿ208C. The reaction rates with resting cells were measured in test tubes or small 50 ml screw-cap glass bottles at 308C and under rotary shaking at 150 rpm. The cells were suspended in the buffer at a desired concentration of cells. After addition of DBT±ethanol solution at the concentration of 1.0 mM, degradation of DBT and formation of 2HBP were measured (see Section 2.6) and the speci®c production rate (mmol/(kg dry cells h)) calculated. 2.6. Analytical methods The cell concentration was determined from the optical density at 660 nm (OD660). For P32C1 strain, a linear relationship between OD and dry cells weight was obtained in the range from 0.08 to 0.7 (absorbance); g dry cells (g/l) 0.39 OD ÿ 0.0037. The cell concentration of IGTS8 was calculated by taking one absorbance unit at 660 nm (1cm optical length) as 0.55 g dry cells/l [18]. The concentration of DBT and other metabolites in growth culture or resting-cell reactions were analyzed by gas chromatography (GC) and high-performance liquid chromatography (HPLC). Liquid samples were acidi®ed to pH 2.0 with 1 N HCl and extracted with ethyl acetate (1.0 vol/vol). A portion of ethyl acetate layer was centrifuged, and 5 ml of the supernatant was injected to a gas chromatography (CP9001, Chrompack International B.V., Netherlands) with a ¯ame ionization detector. The GC was equipped with a stainless still column (3.2 mm 1 m) packed with Silicon OV-17, 2% Chromosorb WAW DMCS 80/100. The ¯ow rate of nitrogen carrier gas was 15 ml/min. The column temperature was programmed from 120 to 2508C, initially kept at 1208C for 5 min and then increased at a rate of 38C/min. The injection and detector temperatures were maintained at 250 and 3308C, respectively. In HPLC analysis with a LC10AD pump and a SPD-M10A variant-wavelength UV monitor (both from Shimadzu, Kyoto, Japan), 10 ml of ethyl acetate layer was applied to a Cosmosil 5C18-AR-300 column (particle size: 5 mm, 4.6 150 mm, Nacalai Tesque). The absorbance of the ef¯uent solution was continuously measured mainly at 280 nm. The mobile phase was 50% of acetonitrile with a ¯ow rate of 1 ml/min. The
retention times of 2HBP and DBT were 5 and 16 min, respectively. Dibenzothiophene sulfone was appeared at 4 min and 20 -hydroxybiphenyl-2-sul®nate (HBPS) which in acidic solution changes to sultine (dibenz[c,e][1,2]oxathiin-6-oxide) had retention time of 1.5 min. Thin-layer chromatography (TLC) was performed on 250-mm-thick, precoated silica-gel plates containing a ¯uorescence indicator (Whatman, Germany) and developed with a 50 : 50 mixture of acetone±heptane. The location of compounds on the plate were detected by use of UV light (253.6 nm). 3. Results and discussion 3.1. Identification of the isolated strain, P32C1 Taxonomical identi®cation of the isolated strain was done by the National Collection of Industrial and Marine Bacteria (NCIMB Japan). Strain P32C1 was a mucoid isolate, grampositive, nonmotile, catalase positive and oxidase negative with short rod shape. Colonies of the strain on TSA plate were yellow. Based on these data this strain was identi®ed as a Corynebacterium sp. 3.2. DBT utilization by growing cells in shaking flask Fig. 2 shows the growth of P32C1 and IGTS8 strains in ¯asks containing 150 ml of Medium A, as well as the concentrations of DBT, 2HBP and intermediate HBPS. In the same time the growth of IGTS8 in BSM medium was also studied. The growth and desulfurization characteristics of IGTS8 strain generally were the same in both the mediums. The growth rate of IGTS8 with the form of DBT±ethanol solution (doubling time of shorter than 10 h) was higher than that with DBT in solid (doubling time of longer than 30 h [19]). As shown in Fig. 2(B,C), P32C1 strain could degrade DBT to a lower concentration and produce 2HBP at a higher concentration than those of IGTS8 strain. P32C1 strain could metabolize DBT to 2HBP also through HBPS intermediate, which accumulates in measurable level, as shown in Fig. 2(D). Synthesized HBPS contained impurities and it was not possible to determine the concentration of HBPS. Thus, Fig. 2(D) showed the measured peak areas corresponding HBPS. These data con®rm that P32C1 selectively desulfurize DBT through 4S pathway. Since the production rate of HBPS, as it was shown for enzymatic reaction of IGTS8 [16], is nearly ®ve times higher than the consumption rate, this explains why HBPS accumulates in the medium. 3.3. Batch culture of P32C1 in jar-fermentor In Fig. 3(A), growth and desulfurization characteristics of P32C1 in the jar-fermentor with the same composition of medium and DBT concentration as in small-scale shaking
S. Maghsoudi et al. / Biochemical Engineering Journal 5 (2000) 11±16
Fig. 2. Growth (A), DBT degradation (B), 2HBP formation (C), and HBPS change (D), during cultivation of P32C1 and IGTS8. DBT with initial concentration of 0.25 mM was used as source of sulfur. Symbols: *, P32C1; &, IGTS8. See text for other culture conditions.
¯ask were shown. In 19 h, 0.25 mM of DBT completely degraded and after 27 h, 2HBP reached to the maximum value of 0.16 mM. The amount of 2HBP that appeared in the medium was less than consumed DBT. It was assumed that some portion of the substrate may dissolve into cell membrane or bind to inert surface [18,20]. We checked the effect of aeration on sublimation and adsorption of 2HBP during 24 h aeration to 1 l uninoculated medium. During 24 h, 12% of 0.25 mM 2HBP was lost. Although the growing cells affect this value, this can be one reason for the discrepancy between the amount of degraded DBT and produced 2HBP. The speci®c degradation rate of DBT and the speci®c production rate of 2HBP in the early growth phase were calculated as 85.0 and 16.7 mmol/(kg dry cells h), respectively. To study the activity of resting cells at different growth phases, cells were collected from fermentor in the time interval shown in Fig. 3(B), washed and resuspended in buffer to OD660 1.0. The conversion of 0.2 mM of DBT after 4 h by these cells are shown in Fig. 3(B) as the percentage of DBT converted and 2HBP formed. This behavior showed that more active cells should be prepared from the late growth phase. 3.4. Degradation of DBT by resting cells
Fig. 3. Time course of culture of P32C1 in jar-fermentor (A). Desulfurization of DBT by the resting-cell of P32C1 from different growth phases (B). *, growth; *, DBT; &, 2HBP; ~, pH.
Formation of 2HBP from biodesulfurization of DBT with various amount of resting cells prepared from late exponential phase of P32C1 in the jar-fermentor was investigated (Fig. 4). The activity of the resting cells was shown as 2HBP
S. Maghsoudi et al. / Biochemical Engineering Journal 5 (2000) 11±16
Fig. 5. Time course of fed-batch culture of P32C1 in jar-fermentor. *, growth; *, DBT; &, 2HBP.
2HBP, although it was shown that 2HBP higher than 0.2 mM retarded growth of IGTS8 and R. erythropolis D1 strains [18,21]. 4. Conclusion
Fig. 4. 2HBP production using cells obtained from jar-fermentor (A). Specific production rate of 2HBP by different amount of cells (B). &, 5; *, 10; &, 20; *, 40; ~, 80 g dry cells/l.
formation rather DBT degradation, since accurate measurement of the DBT concentration at high cell densities was dif®cult [7,18]. Speci®c production rate of 2HBP (mmol/(kg dry cells h)) for higher cell densities were lower, probably due to mass transfer limitations, but the maximum conversions were higher. For IGTS8 strain the maximum reported rate of 2HBP production was 6.1 mmol/(kg dry cells h) [18], but with P32C1 the higher rate of 16.6 mmol/(kg dry cells h) was achieved. In another experiment, the resting cells (30 g/ l) from culture of P32C1 with 0.1 mM DBT could completely convert DBT of 0.5 mM to 2HBP during 30 min (data not shown). The speci®c production rate of 2HBP was 37 mmol/(kg dry cells h). 3.5. Fed-batch culture of P32C1 To study the activity of growing cells during cultivation and extend of 2HBP formation by P32C1 strain, DBT was added fed-batchwise after degradation of DBT, as shown by arrows (Fig. 5). With fed-batch addition of DBT during cultivation of P32C1 in the jar-fermentor containing 1 l medium with glycerol of 5 g/l and initial DBT of 0.25 mM, added DBT was degraded, repeatedly. These results showed that P32C1 strain retained the activity of DBT degradation throughout the culture period, and as well as the possibility of growth at higher concentrations of
A new isolated Corynebacterium sp. strain P32C1 showed better activity and more ef®cient desulfurization of DBT as compared with Rhodococcus sp. IGTS8. Accumulation of HBPS intermediate and detection of small amount of dibenzothiophene sulfone in culture medium con®rmed that this new strain desulfurized DBT via 4S pathway. This means that strain P32C1 could be a much more promising biocatalyst for middle distillate desulfurization as compared to other strains that use DBT as a carbon and energy source and, thus, cause a heating value loss [22,23,24]. The results from fed-batch addition of DBT that showed the possibility of passing through the barrier of 0.2 mM 2HBP during growth phase might be used for production at a high cell density of more active cells. P32C1 also has the ability to desulfurize DBT in the presence of hydrocarbon like n-hexadecane (the paper under preparation). Acknowledgements We appreciate the support of Fellowship Section of UNESCO to complete a part of this work as a collaboration between Sharif University of Technoloy in Iran and Kobe University in Japan. References [1] D.J. Monticello, Riding the fossil fuel biodesulfurization wave, CHEMTECH 28 (1998) 38±45. [2] T. Ohshiro, Y. Izumi, Microbial desulfurization of organic sulfur compounds in petroleum, Biosci. Biotechnol. Biochem. 63 (1999) 1±9.
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[3] J.G. Speight, The Desulfurization of Heavy Oils and Residua, Marcel Dekker, New York, 1981. [4] J.J. Kilbane, K. Jackowski, Biodesulfurization of water-soluble coalderived material by Rhodococcus rhodochrous IGTS8, Biotechnol. Bioeng. 40 (1992) 1107±1114. [5] J.R. Gallagher, E.S. Olson, C. Stanley, Microbial desulfurization of dibenzothiophene: a sulfur-specific pathway, FEMS Microbiol. Lett. 107 (1993) 31±36. [6] T. Omori, L. Monna, Y. Saiki, T. Kodama, Desulfurization of dibenzothiophene by Corynebacterium sp. strain SY1, Appl. Environ. Microbiol. 58 (1992) 911±915. [7] Y. Izumi, T. Ohshiro, H. Ogino, Y. Hine, M. Shimao, Selective desulfurization of dibenzothiophene by Rhodococcus erythropolis D1, Appl. Environ. Microbiol. 60 (1994) 223±226. [8] J. Konishi, Y. Ishii, T. Onaka, K. Okumura, M. Suzuki, Thermophilic carbon-sulfur-targeted biodesulfurization, Appl. Environ. Microbiol. 63 (1997) 3164±3169. [9] S.K. Rhee, J.H. Chang, Y.K. Chang, H.N. Chang, Desulfurization of dibenzothiophene and diesel oils by a newly isolated Gordona strain, CYKS1, Appl. Environ. Microbiol. 64 (1998) 2327±2331. [10] S.A. Denome, E.S. Olson, K.D. Young, Identification and cloning of genes involved in specific desulfurization of dibenzothiophene by Rhodococcus sp. strain IGTS8, Appl. Environ. Microbiol. 59 (1993) 2837±2843. [11] S.A. Denome, C. Oldfield, L.J. Nash, K.D. Young, Characterization of the desulfurization genes from Rhodococcus sp. strain IGTS8, J. Bacteriol. 176 (1994) 6707±6716. [12] C.S. Piddington, B.R. Kovavevich, J. Rambosek, Sequence and molecular characterization of a DNA region encoding the dibenzothiophene desulfurization operon of Rhodococcus sp. strain IGTS8, Appl. Environ. Microbiol. 61 (1995) 468±475. [13] T. Ohshiro, Y. Hine, Y. Izumi, Enzymatic desulfurization of dibenzothiophene by a cell-free system of Rhodococcus erythropolis D-1, FEMS Microbiol. Lett. 118 (1994) 341±344. [14] T. Ohshiro, Y. Kanabayashi, Y. Hine, Y. Izumi, Involvement of flavin
[16] [17] [18]
[20] [21] [22] [23] [24]
coenzyme in dibenzothiophene degrading enzyme system from Rhodococcus erythropolis D-1, Biosci. Biotechnol. Biochem. 59 (1995) 1349±1351. T. Ohshiro, K. Suzuki, Y. Izumi, Dibenzothiophene (DBT) degrading enzyme responsible for the first step of DBT desulfurization by Rhodococcus erythropolis D-1: purification and characterization, J. Ferment. Bioeng. 83 (1997) 233±237. K.A. Gray, O.S. Progrebinsky, G.T. Mrachko, L. Xi, D.J. Monticello, C.H. Squires, Molecular mechanisms of biocatalytic desulfurization of fossil fuels, Nature Biotechnol. 178 (1996) 6409±6418. G. Hanson, D.S. Kemp, Convenient routes to 4,400 -functionalized oterphenyls and 2,2-functionalized biphenyls, J. Org. Chem. 46 (1981) 5441±5443. H. Honda, H. Sugiyama, I. Saito, T. Kobayashi, High cell density culture of Rhodococcus rhodochrous by pH-stat feeding and dibenzothiophene degradation, J. Ferment. Bioeng. 85 (1998) 334± 338. K.J. Kayser, B.A. Bielaga-Jones, K. Jackowski, O. Odusan, J.J. Kilbane II, Utilization of organosulfur compounds by axenic and mixed culture of Rhodococcus rhodochrous IGTS8, J. General Microbiol. 139 (1993) 3123±3129. P. Wang, S. Krawiec, Kinetic analysis of desulfurization of dibenzothiophene by Rhodococcus erythropolis in batch and fedbath cultures, Appl. Environ. Microbiol. 62 (1996) 1670±1675. T. Ohshiro, K. Suzuki, Y. Izumi, Regulation of dibenzothiophene degrading enzyme activity of Rhodococcus erythropolis D-1, J. Ferment. Bioeng. 81 (1996) 121±124. D.J. Monticello, D. Bakker, W.R. Finnerty, Plasmid-mediated degradation of dibenzothiophene by Pseudomonas species, Appl. Environ. Microbiol. 94 (1985) 756±760. S. Frassinetti, L. Setti, A. Corti, P. Farrinelli, P. Montevecchi, G. Vallini, Biodegradation of dibenzothiophene by a nodulating isolate of Rhizobium meliloti, Can. J. Microbiol. 44 (1998) 289±297. A.L. Laborde, D.T. Gibson, Metabolism of dibenzothiophene by a Beijerinkia species, Appl. Environ. Microbiol. 34 (1977) 783±790.
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Alt-rock trailblazers Cold have announced that they will hit the road on "A Different Kind of Tour" this spring, presenting themselves in an all new, intimate element that recent followers and long-time fans alike cannot miss! Each show will feature a very special full-length stripped down set of new songs and fan favorites – …Read More »
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Mega Recruitment For 4.5 Lakh Vacant Posts
Over 4.75 lakh vacancies to be filled soon- Read Here Complete Details
Central Government Mega Recruitment 2020: Union Minister of State Dr Jitendra Singh said that over 4 lakh 75 thousand vacancies will be filled soon. This information was given in a written reply to a question in Rajya Sabha .Candidates who are waiting and preparing for government jobs grab this opportunity as its a Mega Recruitment announced by Union Minister.
राज्यात 'एवढी' पदे रिक्त?…माहिती अधिकारातून उलगडा!
गुड न्यूज! आता होणार 4.75 लाख सरकारी नोकर भरती
Preparation for recruitment of 4.75 lakh government posts, know which departments will get jobs
During the year 2019-20, three recruiting agencies namely, Union Public Service Commission (UPSC), Staff Selection Commission (SSC) & Railway Recruitment Boards (RRB) have made recommendations for recruitment to 1,34,785 posts,"Besides, the SSC, RRBs, Department of Posts and Ministry of Defense have commenced the process for filling up of additional 3,41,907 vacant posts," said by Minister.
This information was given by Union Minister.
Recently, in January 2020, all Ministries/Departments of Central Government have been requested to take time bound action to fill the existing vacancies in respective Ministries/Departments and their attached/ subordinate offices, he has answered, in response to the question based on what measures are being taken by government to deal with unemployment situation in the country and whether any instructions have been issued to all Ministries/ Departments.
Every year huge number of candidates appear for SSC exam, over 13,995 candidates had selected in 2019-2020. Therefore your selection is not so far…..be ready with your preparation as it is a big opportunity to get placed a government sector Don't miss it….. Candidates read the complete details carefully and keep visit us..


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The floorplan developments and "most-requested options" listed in this article convey the overall interests of right now's current inventory house plan purchasers. A versatile-space which can be used for a function most needed by the person / household dwelling in the house at the present time. As such, I'll attempt to provide you with several common developments, as they're, for today's current crop of stock home plan. The room's operate may change over time, because of the family's current needs, but typical uses include an children's playroom, home research, additional storage, and/or an extra bathroom. Renowned constructing designer, Mark Mathis, answers this query and outlines several normal tendencies, as they're, for at this time's current crop of stock house plans. For instance, a house with a traditional model would possibly make the most of a relatively excessive-pitched gable over the storage space of the house. Conversely, a "bonus room" is normally created (by designer's intent) on account of an inherent characteristic of the particular design type.
Large quantities of storage house and enormous walk-in closets are additionally a function that most residence consumers are searching for of their next home. It is that this function that has made the break up-floorplan structure more and more popular with immediately's home consumers. Regardless of those kind items, the split-floorplan layout continues to get pleasure from exceeding recognition with in the present day's group of inventory homeplan purchasers. The mixture of all these kind objects enhance the complexity of developing a stable cut up-floorplan house plan design that meet all of the livability features of at present's home buyer. But now that carriage house plans are readily accessible on-line it can save you your self quite a lot of money and time. Many households now embody two full-time working mother and father, additional exterior work commitments, professional commitments, family commitments, and 2.5 kids of various ages. Then again a number of the websites contain drawings which have already been used for earlier builds and are actually being 'recycled' online. One in every of the most well-liked questions that folks ask when trying to find that perfect home plan is What are (at the moment) probably the most requested floor plan - home features that individuals are on the lookout for?
Some persons are experienced enough, or generally dangerous sufficient, to create their own architectural drawings and instructions. These websites are available for a pair of various causes however you should undoubtedly reap the benefits of them previous to your house improvements. 3: Split-Floorplan Layout - There are two primary sorts of floorplan layouts, that are primarily differentiated by way of their inherent treatment of the master bedrooms suite vs. The first sort of floorplan structure is a standard format whereby the grasp bedroom/suite is often located on the same aspect of the home as the other bedrooms. 1: Livability - The first key feature is that of Livability. Another livability enhancement may be that of incorporating an open floorplan structure that better facilitates the interplay and close relative proximity of the family unit. Both the first advantage and disadvantage of this type of structure relate to the location of the grasp bedroom vs. Alternatively, the split-floorplan structure addresses the shortcomings of the normal structure by physically separating the master suite from the opposite bedrooms, and many occasions, from the more extremely-trafficked public areas such as the kitchen, great room, and eating space.
The primary advantages of the sort of structure is that of its relative privateness and sound-deadening benefits that such a physical room association can present. For a lot of younger households, that can imply a re-purposing of a formal dining room into a kids's play area or much-needed house office. These kind products can assist to enhance the quality of life of the house house owners by minimizing the time and associated prices related to maintaining a house. This explicit question is one which is quite difficult to answer, in absolute phrases, since every person/family could be in a completely totally different stage of life (senior vs. In these situations, you can be assured that a designer has already developed a inventory dwelling plan design to fit your needs. Additionally, the hardy board siding does have to be painted or re-completed, as any dirt, etc. can merely be washed away with minimal effort. A few of the most effective website can even inform you the precise materials that you just need and the precise instruments that might be required to complete the job efficiently.
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Introducing Phil Nash, Developer Advocate for C++, Objective-C and Swift tools
Phil Nash has joined us recently as a Developer Advocate for C++, Objective-C and Swift tools. Here we talk to Phil about his experience and interests and introduce him to all of you. Twitter: @phil_nash Blog: levelofindirection GitHub: philsquared A: … Continue reading
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Lead generation is the process of attracting customers to be interested in your company or product and make them want to request more information from you. Lead generation is critical for the growth of any business. The buying process (sometimes referred to as the "buyer's journey") has changed and business owners must find new ways to reach their target audience. ... Read More
Top 5 Ways to Grow Your Email List with Instagram
Instagram is not just for posting selfies, it can help increase your email list for your small business! Email marketing is a very effective way to get your business's content out in a matter of seconds to a large group of people at the same time. Today, social media is a critical aspect in your digital marketing mix towards growing ... Read More
How to Make Existing Content More Valuable for SEO
Every piece of content your business has produced is more valuable than you think. Finding content that can be repurposed can be more cost-effective and time-efficient than creating brand new content from scratch. Repurposing content allows you to introduce your brand/company to a new audience and can help to establish authority on additional platforms you're not currently on. When you ... Read More
Top 10 Tactics to Help Get Free PR for Your Small Business
Public relations is the relationship between the public and a company or other organization or a famous person. The goal of a public relations campaign for a business is to gain/increase awareness by the public about a newsworthy "story" having to do with your business. If you want to gain press coverage about your business but don't have the funds ... Read More
Email Marketing Tips to Grow Your Small Business
Email marketing helps businesses connect with their audiences to help promote their brand and increase sales. The purpose of this type of marketing is to acquire new customers, increase sales, enhance customer relationships, and increase awareness about their company or brand. Below we've listed the most effective email marketing tips to grow your small business. 1. Build a High-Quality Email ... Read More
Top 9 Persuasive Advertising Techniques
Companies use advertising as part of their marketing plan to build awareness and ultimately increase sales of their products and services. Persuasive advertising is used to gain people's attention, in an attempt to convince a potential customer to try your product for a number of reasons. The key to persuasive advertising is to: fully understand your target markets' wants and ... Read More
Affordable Online Advertising Tactics to Increase Website Traffic
One of every company's goal is to get as much traffic to their website and social media as possible. Generating more traffic ultimately results in more conversions and sales. Online Advertising is an affordable and effective way to increase traffic and generate sales. Here are several effective and affordable online advertising tactics to increase your website traffic. Influencer Marketing Influencer ... Read More
8 Ways to Optimize and Enhance Your Website
Properly designing your website can make all the difference when trying to convert visitors to customers. If your website isn't properly laid out or optimized, people will leave it almost immediately. People's attention spans when looking at content online is extremely short, so it's incredibly important that your website makes a good first impression and has what it takes to ... Read More
Why are Backlinks Important for SEO?
Backlinks have become increasingly important for search engine optimization. Backlinks are when one website links to a second website. Essentially, they are links on another website that directs towards your website. The number of backlinks indicates to search engines the importance and value of a certain website. Quality backlinks can also increase website traffic. The more places your website address/link ... Read More
7 LinkedIn Tips for Increasing Followers
Linkedin is about making connections with people (aka "leads") you know, or want to know. One of the most valuable goals on LinkedIn is to grow a following. Increasing your following leads to a greater organic reach, meaning more people will be looking at the content you post. Below we have come up with 7 LinkedIn tips for increasing followers. ... Read More


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Thinking about quitting your job and starting your very own home-based business? Great! Thinking about how you're going to go about taking on this big venture? Hmmm… That's a really great question, right? You can either take control of your life today and start doing what it takes to make this dream come true, or you can keep dreaming, it's up to you. If you choose to take control, take the first step by choosing one of our many Lists so you can start to reach out to those who can help you reach your goals.
The sales lead component of D&B Hoovers includes information on over 120 million business records covering over 1,000 industries in 190 countries, making it more comprehensive than any other option on this list. It provides the most robust list segmentation capabilities and is ideal for fine-tuned searching within geographic territories. D&B Hoover also offers seamless lead integration with many CRMs, though they aren't listed on the website.
Our training: We offer live dials training calls weekly. We actually call our own leads and prospect like were a distributor looking for new reps. We usually have hundreds of people on our conference call, they are muted and are able to listen as we discuss the various options with the prospect. Every one of these calls are slightly different and after every time there is a call made we do an analysis of the response and break down the elements of the call. This is what training is about! Imagine listening to this live this is not theory but it's real training. The best of all, these calls are absolutely free to our customers and our future customers.
Pay Per Click or PPC can be very effective in the right hands. If you do it the wrong way it can be a fast track to financial ruin. Unlike Facebook Ads where you can see how effective your ads are within minutes, Google AdWords are much less responsive and you can use up a lot of cash before you hit the winning combination. Concentrate on being very focused and specific. If you are not, you will get lots of clicks on an ad which sounds right, but results in low conversion rates when people find that it's not exactly what they are looking for.
I give you two great suggestions AND a really easy script inside the below video but I really want to stress that MOST people buy MLM Leads with the wrong expectation. They think they should be lay down sales and that is just not the case typically. You still need to bone up on our closing and communication skills as well as your follow up skills. Don't just make one call and expect that to be it, in the video I suggest a long term follow up strategy that some of you would REALLY get more sales if you implemented.
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Video – Having video on a page can work for you or against you depending on what information is provided in the video posted on the lead capture page. The video should NOT be a business presentation. Again, it should pick the interest or curiosity of the prospect to entice them to complete the webform so that you can capture a lead. General testimonials can work well in video. They should not be specific to any product or company by name but should relate to the prospect how it has worked for them. Video on a lead capture page is an option; not a requirement. We've been successful in generating targeted mlm leads without video on the lead capture page.
Biz Opp Leads are leads that we have captured when people fill out a form through a number of home business lead websites. This means that these are people who are interested in learning about any and all new business opportunities that are available, which is exactly what you're looking for, right? Every new lead that signs up has agreed to provide their name, email address, physical address, and phone number. It simply doesn't get any easier than this, so what are you waiting for? Call us right now so you can start making the contacts you need for success.
At the other end of the spectrum is buying leads. This is also not the best option since it can be very expensive and may result in leads that may not actually be interested in your products or business. These are not great leads, either. The best leads will always be the ones you generate yourself—people who have shown some sort of interest in what you have to offer.
MyAutoPilot Traffic sends traffic to you on a pay per click basis. Each click we send to fill your order acts to send a business opportunity seeker to your lead capture page. Each time we send a visitor to your lead capture page, we send you an email notification so that you have an indication of when traffic is being directed to your lead capture page.
Have you ever thought about running your very own Classified Ads site? Imagine not having to worry about hosting, maintenance, backups, or any of the other hassles that comes with running a classifieds site. With the Youmongus Ad Network we will setup your own classifieds site, with a domain name of your choice, and you'll be able to charge for ads, run banner ads, and build a large and successful ad site.
Local business opportunity leads are available by telephone area code of where you want your leads. You can have as many area codes assigned to your order as you desire. At any time you can add or remove telephone area codes by contacting us. The more telephone area codes you provide us, the faster your local business opportunity leads order will fill. Click here to order.
How do you choose mlm leads that are genuinely interested in starting a home business, just like you? Where can you find an mlm lead generation company that takes you and your business seriously—one that actually cares about your success whether you are a leader with 50,000 people in your team or just starting out? You've come to the right place—a place where we make it easy.
This is simply the best BANG FOR YOUR BUCK membership available anywhere, imagine solving all of your lead generation needs with one low cost membership! Try Out Our System For 3 Days For Only $2.99, If You Are Not Satisfied For Whatever Reason Then Cancel At Anytime! Sign Up Now To Get Access To Your 1000 Daily Business Opportunity Leads, Plus Your Software And Information Bonuses And Start Making More Money Today!
One of the unique features of Sales Navigator is that it allows users to directly contact prospects using LinkedIn's InMail feature. This allows users to send messages to prospects they aren't already connected to on LinkedIn and see when recipients have read their email. You're allowed you to send 20 InMail messages per month to reach new prospects directly on LinkedIn. The Team plan allows you to send an additional 10 InMail messages per month.
We specialize in High Quality Business Opportunity Leads for your business or affiliate program. Our goal is to provide you with the highest quality business opportunity leads recruitment and other networking services at affordable prices. If you need to promote your website, gather signups to add to your downline, or just be KNOWN on the net, we can help!
Real Time Redirected Short Form MLM Leads provide an advantage over regular non-redirected leads, as the prospect is able to find out more details about your opportunity, while their interest is at its peak. They are currently searching online, came across our website, and now they are on yours. No delays, no waiting for you to schedule an appointment, just instant gratification NOW when they are actively seeking it. If you've got a great online movie or business presentation about your business, then Redirected Short Form Leads could be exactly what you are seeking!
Users like access to the LinkedIn network through search filters to identify decision-makers at specific companies. Users also like that it allows you to reach out through InMail, which is more likely to create engagement than other types of communication like email or cold calls. Some users said that company names are sometimes misspelled and the filters don't always work correctly. To read in-depth user reviews, visit our Sales Navigator reviews page.
Buying MLM leads can be a great way to get experience and possible signups. However, it isn't something I always wanted to have to do, I wanted to learn how to generate my OWN MLM leads, IF you want to learn that skill, be sure to ask my business manager about our open mastermind/workshop we are putting on in Florida in January. You can reach him at [email protected]
It's estimated that in under 2 years almost 90% of web traffic by volume will be video. If you have the skills, or can hire a good SEO specialist, it is possible to rank a sales video on Page 1 of Google with relative ease depending on how competitive your niche is. Put a visible call to action in the title which appears in the search engine results page, and link the YouTube video through to your web site and with luck you should have a nice flow of leads as long as your video is listed on Page 1.


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Fractal Bookmark 2.pdf
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How To Make Easy Money Online Now
Without it, you've no place to filter all that traffic and people interested in what you have to say. I talk about it in my guide to selling clothes online! Here's a list of legitimate ways to make money online fast.
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Week #1 Farm Management Bracket Soil Fertility Bracket Farm Safety Bracket Farmer and Farmland ... minutes Take the Soil Fertility online course (ENR5270) General Overview of Farm Hazards Estate Planning: ...
COVID-19 Updates
Is my extension office closed? Will private pesticide and fertilizer deadlines be extended? I have ... not received my new pesticide/fertilizer applicator card – what do I do? I am a pest control ... until further notice. Educators are still remotely serving the counties. "While our physical offices ...
EPN Community Connections Blog- March 2020 EPN Student Spotlights
By Rylie MacDonald Earlier this month, we switched to an online webinar platform for our March EPN ... climate to water resource managers. of the School of Environment and Natural Resources. Rylie MacDonald, ... students studying and interested in water related research and careers. As student affiliates of the Water ...
2017 Webinars- Profiles in Service
2017 Webinars- Profiles in Service Please consider participating or viewing our quarterly OCVN ... posted later. December 13 - 12 noon-1:00 pm- Ohio Dragonfly research, presented by Jim Lemon, OCVN ... will be using iNaturalist for photo submissions. Many of our OCVN's volunteer in good Odonate habitats ...
Full Bracket
(March 30-April 3) Hemp Direct Marketing Farm Management Forages, Pastures, and Grazing Farm Safety Week ... tip-off Stress Management Tips & Resources Farm & Rural Health Task Force Watch Replay Watch Farm ... Business Management Watch Replay Week #2 March 30-April 3 Monday, March 30 Bracket: Hemp Return to Top ...
COVID-19 Guidance
will unfortunately not be a factor in approving continuing activities. To be sure, our research will be ... Management & Budget- 3/20/20 Clarification on Critical Research- 3/19/20 CFAES Research Questions as ... the OSU-CFAES research community, here. Request Research/Extension Exemptions Research and Extension ...
EPN Zoom Webinar- April 15, 2020 Ohio State's Celebration of the 50th Anniversary of Earth Day
point in the attention paid to our global environment and a precursor to many critical environmental ... respect to environmental advances, our greatest challenges may lie ahead. Join this moderated, online ...
EEOB 3498 Undergraduate Research
EEOB 3498- Undergraduate Research aims to provide opportunities for undergraduates to conduct ... supervised independent research in social behavior. Research projects will focus on working with live ... classes with observational research individually conducted outdoors by students. This course is ...
Perfect Stay-at-Home Projects
to review the related guidelines. NONDISCRIMINATION NOTICE CFAES provides research and related ...
Agriculture & Natural Resources Madness- Tournament of Education
director of OSU Extension. "While our 'tournament' is being loosely tied to March Madness, it's not ...