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The aim of the event is to bring together the disciplines of RACHP and ORC (Organic Rankine Cycle) to explore their common challenges and learn from each other about the solutions being developed to tackle them.
Over three days delegates will explore research and developments in the closely related fields of power cycles, working fluids, and RACHP applications. They will take away insights into new opportunities to deliver efficient, sustainable and environmentally friendly solutions.
In the 150 years since William Rankine developed his power generation cycle, the world has changed enormously in both terms of the technology available and the political landscape. Sustainability and environmental issues are now a major issue and a driving force behind legislation.
In an era where there is increasing demand for energy while at the same time fossil fuels are being phased out, Rankine's power generation cycles will only become more important. While William Rankine would recognise the underlying science that is currently in use the application of his technology is vastly different. This conference will spend time exploring the legacy of William Rankine, looking at his ground-breaking research to see what modern engineering can learn from this great Victorian innovator. It will also look to the future and offer a forum where the different disciplines of power generation, refrigeration, air conditioning, and heat pumps can come together to discuss their common challenges and learn from each other about the different solutions that are being developed to tackle them.
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The Institute of Refrigeration is proud to host the International Institute of Refrigeration Conference "Rankine 2020 - Advances in Cooling, Heating and Power Generation", timed to coincide with the bi-centenary of the birth of William Rankine. It will mark the legacy of WJM Rankine whose name is now synonymous with power generation cycles. The event is being organised in association with the Knowledge Center for Organic Rankine Cycle (KCORC) and IESIS.
Sponsorship packages are available for companies to raise their profile in this sector and take advantage of the international exposure this event will attract. Details available here.
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YPG/PKK missiles hit residential area in SE Turkey
ŞANLIURFA-Anadolu Agency
At least two children were injured as anti-aircraft missiles fired by YPG/PKK terrorists in Syria hit three buildings in southeastern Turkey.
The missiles struck three houses in Şanlıurfa province's border district of Ceylanpınar.
Two siblings aged 13 and 15 were injured while they were playing outside their house.
They were rushed to Ceylanpınar State Hospital. No further details of the nature of injuries and condition were provided yet.


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While admitting it's highly unlikely, injured Hawthorn star Tom Mitchell still won't completely rule out making a shock return this season.
The reigning Brownlow Medallist broke his leg at a pre-season training session in January with the Hawks suspecting he will miss the entire 2019 campaign.
"It's really hard to say, I would say it's (a return) highly unlikely," Mitchell told SEN's Time On.
"But never say never, I always want to hang onto a little bit of hope. If the team's playing finals, I would love to be a part of that.
"I won't completely rule it out, but it will be up to the medical team in the end."
Mitchell, 25, says his rehabilitation is going as planned but a fixed return date hasn't been set for the prolific midfielder.
"Everyone recovers differently at different rates, but at the moment I'm on the bike, I'm in the pool, I'm in the gym and I'm feeling pretty good walking around with little pain," he said.
"I'll say it's all progressing pretty nicely at the moment and in terms of getting back to training and playing, it's really too hard to tell.
"In terms of making a full recovery then that won't be an issue at all."
The Hawks open their 2019 campaign on Sunday against the Crows at the Adelaide Oval.


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Search the Community
Showing results for tags 'cockle'.
Found 8 results
Cockle Shell
I_gotta_rock posted a fossil in BivalvesThis was carved carefully from a block of matrix that fell out of the cliffs into the bay. Of the dozens that I found, this was one of the few that did not completely fall apart what it was separated from the surrounding sand. Though thicker than many shells in the same chunk of sand, they are extremely soft in this location and incredibly fragile. Donated to the Delaware Museum of Natural History.
Trachycardium emmonsi
sixgill pete posted a fossil in BivalvesAvery nice double valve cockle. Single valves are not uncommon at this site but double valvers are very uncommon for this species.
The Cockles: Common vs Lagoon
Max-fossils posted a topic in Questions & AnswersHi all, So the Cerastoderma genus has (in addition to a few other fossil species) two extant species: C. edule (common cockle0 and C. glaucum (lagoon cockle). Both of these species appear (both fossil and modern) here in the North Sea, and their Eemian fossils are common finds at the Zandmotor. Now I have always been told, and read in most of my books, that the difference between them is that: -> if you draw a vertical line from the umbo downwards, C. edule is pretty much symmetrical while C. glaucum will have one side more stretched out. As can be seen in the picture above. Pretty straightforward. Plus, this is what I explained in one of my old Instagram posts: But, while searching a bit around, I just now saw on the Wikipedia website a picture of a symmetrical cockle that they claim is C. glaucum. And WoRMS also has some pictures of some more or less symmetrical cockles for the lagoon cockle! So I am very confused... What is the difference then between the two species? Looking forward to your answers! Best regards, Max
Blister Pearls
fossilnut posted a topic in Fossil IDI have never found pearls before so I am posting for confirmation. I have seen modern blister pearls at rock shows. Also wondering if these are fossil or modern day. The background for these finds is my wife had oysters locally and one had what we believe is a blister pearl. She seems to have an affinity for pearls as she has found 4 pearls (not blister)--2 in mussels and 2 in oysters. A few days later while walking the beach I found the large 1 1/2 inch pearl in a piece of quahog (Mercenaria) shell. Then I found other quahogs with interior coatings that differed from the normal shell. These had small raised bumps or "pimples". Then my wife found a cockel shell that had a small cluster of pearls. i wonder if these are possible Pliocene fossil pearls rather than recent? There are Miocene/Pliocene fossils shark teeth and fish material. Are these in fact blister pearls and how do I preserve them? Thanks for looking at these.First picture is modern oyster with blister pearl. Quahog blister pearl--fossil pearl?
Cerastoderma edule
Max-fossils posted a fossil in BivalvesA common cockle from the Zandmotor. A very common species.
Cockle: Cerastoderma
Max-fossils posted a gallery image in Members Gallery
From the album @Max-fossils 's Zandmotor FindsA nice cockle found on the Zandmotor. Species: Cerastoderma edule. A very common species, not really worth picking up if you already have some.
Info about a fossil from your knowledgable perspectives?
gowcoizer posted a topic in Fossil IDHello again. So the story behind those is that I found the smaller one when I was a child.. may be around 15 years ago and I kept it to bring me memories for the adventurous spirit from those times. This year, my brother found the bigger one.. it is similar but a little bit larger so I decided for a first time to investigate the origins. I found the little one in area with trees and leafs on the ground and somehow I think I stepped and dug it from the ground cause the angle of the soil was too sheer. The area is central Bulgaria, seems like some kind of cockle ? Seen more like this ? The closest sea is 180km away... is that a proof for area being full of water may be long time ago?


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text: tempobet tipobet Instagram Profiles Peoples Republic Of Cork Discussion Forums - View Single Post - The Official Celtic Thread Thread: The Official Celtic Thread View Single Post #17 07-03-2007, 09:22 PM Philby Join Date: Feb 2003 Posts: 21,187 I was going to say it. Bringing Milan to extra-time would be a great achievement but just how much energy with the Celtic side have to expend to do it? You've got to think that they'll tire badly as the game goes on. Would be great to see them pull of a result, it would be some coup. Philby View Public Profile Find all posts by Philby


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We are currently looking for a Graduate Civil Engineer to join a niche civil engineering consultancy to work in their Surrey office, located in Redhill. Candidates will be working on technically challenging projects that will help grow their knowledge. You will be working within a team, reporting to a senior staff member, focusing on the delivery of a variety of civil engineering projects to their clients.
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Discovery of a widely distributed toxin biosynthetic gene cluster
Contributed by Jack E. Dixon, February 10, 2008 (received for review February 5, 2008)
Bacteriocins represent a large family of ribosomally produced peptide antibiotics. Here we describe the discovery of a widely conserved biosynthetic gene cluster for the synthesis of thiazole and oxazole heterocycles on ribosomally produced peptides. These clusters encode a toxin precursor and all necessary proteins for toxin maturation and export. Using the toxin precursor peptide and heterocycle-forming synthetase proteins from the human pathogen Streptococcus pyogenes, we demonstrate the in vitro reconstitution of streptolysin S activity. We provide evidence that the synthetase enzymes, as predicted from our bioinformatics analysis, introduce heterocycles onto precursor peptides, thereby providing molecular insight into the chemical structure of streptolysin S. Furthermore, our studies reveal that the synthetase exhibits relaxed substrate specificity and modifies toxin precursors from both related and distant species. Given our findings, it is likely that the discovery of similar peptidic toxins will rapidly expand to existing and emerging genomes.
Streptolysin S (SLS), from the human pathogen Streptococcus pyogenes, is a ribosomally synthesized, secreted toxin responsible for the classical β-hemolytic phenotype of bacterial colonies grown on blood agar media (1, 2). S. pyogenes is associated with a wide spectrum of diseases ranging from simple pharyngitis to life-threatening necrotizing fasciitis. The expression of SLS promotes virulence in animal models of invasive infection (1, 3). The molecular structure of SLS has remained elusive for more than a century; however, SLS is known to be an oxygen-stable, nonimmunogenic cytolysin (4). The gene locus responsible for SLS biosynthesis (streptolysin S-associated genes, sag) was recently identified through transposon and targeted mutagenesis coupled with heterologous expression (2, 5–7). The sag locus consists of nine genes (A–I), and allelic exchange mutagenesis has shown sagA–G to be essential for the cytolytic phenotype of S. pyogenes. The first gene, sagA, encodes a 53-aa protoxin (Fig. 1 A). SagA is followed by a set of three genes (sagBCD) exhibiting low sequence identity to three genes found in the Escherichia coli mcb gene cluster that produces the DNA gyrase inhibitor microcin B17 (MccB17) [supporting information (SI) Fig. S1] (5, 8). SagBCD encode a cyclodehydratase (SagC; 13% identical to McbB), dehydrogenase (SagB; 22% identical to McbC), and a "docking" protein (SagD; 18% identical to McbD) (Figs. S2–S5). The final genes in each cluster are dedicated ATP-binding cassette (ABC) transporters.
Analogous to the sag locus, the first ORF of the MccB17-producing gene cluster mcbA encodes a 69-aa precursor protein (9, 10). Walsh and colleagues (5) have shown that McbA is extensively posttranslationally modified by the McbBCD synthetase complex. These modifications convert four serines and four cysteines into oxazole and thiazole heterocycles, respectively (Fig. 1 B and Fig. S1). Unmodified McbA exhibits no effect on DNA gyrase (11).
Results and Discussion
Heterocycle Formation by SagBCD.
Given similarities in organization of the mcb and sag gene clusters and the observed homology of SagBCD to McbBCD, we hypothesized that the SagBCD synthetase complex would serve to posttranslationally modify a toxin precursor in the same manner as McbBCD. The sagBCD genes were cloned individually and purified as fusions to maltose-binding protein (MBP). Because numerous attempts to observe SagA by mass spectrometry failed, we used McbA, which is amenable to mass spectrometry, to detect and confirm heterocycle formation by SagBCD (12). After removal of the MBP tag, recombinant McbA has a calculated molecular mass of 6,293 Da due to addition of Gly-Ser-His to the N terminus. For each heterocycle formed, a loss of 18 Da (oxazoline/thiazoline) or 20 Da (oxazole/thiazole) is expected from the parent peptide (Fig. 1 B). Treatment of McbA with recombinant SagBCD resulted in the formation of four new masses differing from the precursor peptide by multiples of 20 Da (Fig. 1 C and D). These masses are within error for linear mode MALDI mass spectrometry and correspond to heterocycle formation at four residues of McbA. The fourth heterocycle was not visible when the synthetase concentration was reduced (data not shown), and only unmodified McbA was observed when SagBCD was omitted from the reaction. These results provide experimental evidence that SagBCD functions in a manner analogous to McbBCD.
SagBCD Converts SagA into a Cytolysin.
Because recombinant SagBCD was active in vitro, we next sought to confirm that SagA could be transformed into an active cytolysin in a SagBCD-dependent manner. SagA was produced as an MBP fusion protein and then subjected to modification by the addition of SagBCD in an in vitro synthetase reaction. After this reaction, samples were tested for lytic activity against sheep erythrocytes. In this assay, SLS extracted from S. pyogenes cultures caused rapid hemolysis (data not shown). The addition of SagA alone failed to induce lysis, but robust hemolysis was observed after treatment with SagBCD (Fig. 2 A). All three synthetase proteins were required for the lytic phenotype, indicating a cooperative role in toxin maturation.
To determine whether SagBCD-treated SagA exhibited a broader cytolytic phenotype characteristic of the native SLS toxin, reaction mixtures were applied to HEK293a cells. Cells treated with samples containing both SagA and all of the synthetase components progressively lost their focal contacts and eventually detached from the tissue culture wells (Fig. 2 B). Actin staining revealed massive cytoskeletal collapse consistent with severe membrane damage. HEK cells incubated with solutions missing any component of the synthetase complex, or the protoxin precursor, were indistinguishable from untreated cells (Fig. 2 B). Lactate dehydrogenase (LDH) release into the media provided quantitative confirmation that cytotoxicity required both the SagA substrate and the SagBCD complex (Fig. 2 C). SagA contains seven cysteines that serve as potential sites for thiazole formation by SagBCD. Mutation of all seven cysteines to serine (SagA-panC/S) completely abolished cytolytic activity, indicating either that SagA-panC/S is not recognized as a substrate by SagBCD or that the membrane-damaging phenotype requires cysteine modification. Taken together, these experiments describe the first in vitro reconstitution of SLS activity. Furthermore, we demonstrate that SagBCD functions similarly to McbBCD by installing heterocycles on a peptidic toxin precursor.
The sagBCD Gene Clusters Are Widely Distributed Among Prokaryotes.
The similarities between the SLS and MccB17 biosynthetic operons were intriguing, given that (i) SLS is produced by a Gram-positive organism whereas MccB17 is from a Gram-negative organism and (ii) microcins have heretofore been classified as unique to Gram-negative bacteria (13). This suggested that other prokaryotes could use related machinery to introduce Ser/Thr/Cys-derived heterocycles into a wide variety of ribosomally produced peptides. We therefore initiated a comprehensive survey of the public genomic databases to identify related biosynthetic gene clusters (14). This search revealed that similar gene clusters are widespread among prokaryotes, most of which are not associated with a function (Fig. 3 A). The genes encoding the SagB-like dehydrogenase (Fig. S2), SagC-like cyclodehydratase (Fig. S3), and SagD-like protein (Fig. S4) were present as adjacent ORFs in a diverse group of prokaryotic organisms spanning six phyla. The SagC and SagD orthologs were also sometimes found fused as a single ORF (Fig. S5).
Of particular interest is the fact that gene clusters that highly resemble sagA–I are present in major mammalian pathogens such as Clostridium botulinum, Listeria monocytogenes, and Staphylococcus aureus RF122 (Fig. 3 A). Similar gene clusters are also found in distantly related prokaryotes, such as cyanobacteria (15, 16) and archaea (e.g., Pyrococcus furiosus DSM 3638). Other family members are found throughout proteobacteria. For instance, a gene cluster identical in arrangement to E. coli mcbA–G is present in the plant symbiont Pseudomonas putida KT2440 (13). P. putida colonizes the nutrient-rich rhizosphere of plants and induces plant growth while secreting antibiotics to limit the growth of competing soil bacteria (17, 18). Furthermore, two other plant symbionts, Bacillus amyloliquefaciens FZB42 (Bam) and Bradyrhizobium japonicum USDA110, encode analogous synthetase proteins. Bacillus thuringiensis harbors a similar operon; although not a plant symbiont, B. thuringiensis is of agricultural interest owing to its ability to secrete toxins that are effective against a variety of pests, such as moths, butterflies, flies, mosquitoes, and beetles (19). The conservation of this synthetase gene cluster across prokaryotes—in particular, among both pathogenic and symbiotic bacteria—suggests that these gene products play an important role in the establishment of a survival niche for these organisms.
Presence of Protoxins Across Prokaryotic Lineages.
McbA and SagA encode protoxins of 69 and 53 residues, respectively (Fig. 3 B). These are extremely short ORFs, which many gene-identification algorithms do not always recognize. For this reason, manual ORF searches were performed in the intergenic regions for each biosynthetic cluster identified. Short ORFs encoding proteins of 50–70 residues that are rich in Cys/Ser/Thr were present in all organisms containing sagBCD-like genes as adjacent ORFs (Fig. 3 B). Although the functions of these potential protoxins are not known, based on similarity to McbA and SagA, we speculate that some will function as membrane-damaging agents (e.g., S. aureus and L. monocytogenes) or DNA gyrase poisons (P. putida). Secondary metabolites produced by biosynthetic clusters such as these have historically been an abundant source of pharmaceuticals. The bacterial products of these gene clusters are potential candidates for novel antibiotic design as well as promising targets for vaccine development.
Most of the gene clusters identified contain ORFs encoding additional modifying enzymes, suggesting that, in addition to heterocycle formation, these protoxins will undergo further modification. For example, clusters containing acetyltransferases and lantibiotic dehydratases, such as the goadsporin-producing organism Streptomyces sp. TP-A0584 (20), will likely produce toxins with acetylated and dehydrated amino acids (Fig. S1). Furthermore, methylated toxins are expected from gene clusters with methyltransferases. There are also gene clusters containing radical S-adenosylmethionine (SAM)-like proteins, which catalyze the formation of many types of chemical linkages in complex natural products (21). Inclusion of these ancillary enzymes is expected to dramatically increase the chemical diversity of the toxins, perhaps for niche purposes. The cyanobacteria Prochloron didemni (15) and Trichodesmium erythraeum IMS101 (16) have been previously identified as sources of cyclic peptides with Ser/Thr/Cys-derived heterocycles (Fig. S1). The sag-like biosynthetic clusters of T. erythraeum and P. didemni harbor an additional gene encoding a putative serine protease (Fig. 3 A). Although the exact function of this protein is unclear, it is proposed to catalyze a head-to-tail macrocyclization reaction by using the N terminus of a peptide, instead of water, to hydrolyze the acyl-enzyme intermediate. After this reaction, the volume occupied by the toxin is much smaller and may eliminate the need for an elaborate export system. The lack of ABC transporters in these gene clusters supports this notion (Fig. 3 A). Based on a similar arrangement of genes, we hypothesize that Rhodobacter sphaeroides, Lyngbya sp. PCC 8106, and Microcystis aeruginosa will also form macrocyclized peptides.
The Heterocycle Synthetase Complex Displays Functional Promiscuity.
A gene cluster that is highly similar to the sag cluster was found in the pathogenic bacterium C. botulinum (Fig. 3 A). The organization and amino acid sequence of proteins present in the C. botulinum cluster closA–I displays the highest similarity to sagA–I. The protoxin, which we have designated ClosA, shares many characteristics with SagA (39% identical) including the presence of numerous adjacent cysteines, other conserved heterocyclizable residues, and a putative protease site for removal of a leader peptide (Fig. 3 B). To explore the possibility that closA encodes an SLS-like cytolysin, recombinant MBP-ClosA was prepared and subjected to in vitro synthetase assays with SagBCD. Hemolysis was observed only in samples containing both ClosA and the SagBCD synthetase complex (Fig. 4 A). Microscopy revealed that cytotoxicity in HEK cells treated with ClosA plus SagBCD was identical to cells treated with SagA plus SagBCD (Figs. 2 B and 4 A). These data demonstrate that SagBCD accepts substrates beyond its cognate peptide, SagA.
Given the ability of SagBCD to accept McbA and ClosA as alternative substrates, we assessed whether other synthetases were permissive in their substrate selectivity. To this end, the heterocycle-forming synthetases from E. coli (McbBCD) and the thermophilic archaeon P. furiosus (PagBCD, identity to corresponding Sag: 17–24%) were prepared as MBP fusions. SagA and ClosA were then tested for erythrocyte lytic activity after reaction with McbBCD and PagBCD. The PagBCD complex efficiently converted both SagA and ClosA into a hemolysin (Fig. 4 B). In contrast, the McbBCD complex converted only ClosA into a potent lytic species. It is intriguing that McbBCD accepted and converted an alternate substrate into a cytolytic factor given that its biological function is to produce a DNA gyrase inhibitor (22). Therefore, we conclude that the mechanism of toxin action is governed by the protoxin amino acid sequence and that the synthetase proteins are functionally redundant. The extent of substrate tolerance and the kinetics and selectivity of ring formation require further investigation.
In sum, our bioinformatic survey has uncovered the presence of sag-like gene clusters in a myriad of prokaryotes, leading us to define a new class of bacteriocin. This class is characterized by a biosynthetic gene cluster that encodes a small protoxin and three adjacent synthetase proteins that endow an organism with the ability to cyclize Ser/Thr/Cys residues from a ribosomally synthesized protoxin scaffold. The finding that the heterocycle synthetase genes are exclusively found as adjacent ORFs will facilitate the identification of orthologous biosynthetic clusters in emerging genomes. Using the protoxin and synthetase proteins from the human pathogen S. pyogenes, we demonstrate in vitro reconstitution of SLS activity. Furthermore, we show that the synthetase enzymes are functionally redundant and catalyze heterocycle formation on alternative substrates despite their significantly distinct evolutionary lineages. Our data suggest that all of the newly identified gene clusters are responsible for the production of bacteriocins containing at least one Ser/Thr/Cys-derived heterocycle. Many of the gene clusters encode ancillary modifying enzymes that will install further modifications onto the bacteriocin, thus increasing the chemical diversity of this family. Further insights into this bacteriocin family will lead to the identification of novel targets for antibiotic and vaccine development.
Materials and Methods
Additional Details.
See SI Text for additional methods.
The amino acid sequences of SagBCD from S. pyogenes (2) and McbBCD from E. coli (23) were initially used as a BLAST query against the nonredundant protein sequence database of the National Center for Biotechnology Information (14). Highly similar proteins were identifiable in the firmicutes phylum, specifically in C. botulinum, S. aureus RF122, and L. monocytogenes. The surrounding ORFs for each identified gene were analyzed for the presence of all three synthetase enzymes by using the Genomic Context feature of Entrez Gene and the SEED tool from The Fellowship for the Interpretation of Genomes. Gene clusters that contained all three synthetase enzymes were also found to contain other bacteriocin-type operon components, such as a probable protease, immunity protein, and ABC transporters. In cases where the automatic annotation algorithms do not identify the smaller toxin structural genes (e.g., Listeria, ListA), manual inspection of all predicted ORFs in the local intergene region was performed, and these were subsequently scored by using ClustalW alignment to known toxin structural genes from S. pyogenes and E. coli. Streptococcus iniae has been shown to contain a gene cluster nearly identical to S. pyogenes and was therefore not used for bioinformatics searching (24, 25). Furthermore, another Streptococcus species, Streptococcus dysgalactiae sp. equisimilis, contains a SagA and SagB homolog (3). Because of an incomplete genome sequence, we cannot confirm the presence of SagC–I at this time.
Wider subsequent searches of the nonredundant protein sequence database (National Center for Biotechnology Information) were initiated using all proteins confirmed to have a sag-like gene locus (with bcd genes located directly adjacent to or within a few ORFs of each another). A lower limit of 30% amino acid similarity was chosen as a threshold to assign a candidate protein as being homologous (BLOSUM30 matrix). This second search identified gene clusters from three other organisms that produce a heterocycle-containing bacteriocin with a known structure (Fig. S1), Streptomyces sp. TP-A0584 (goadsporin) (26), P. didemni (patellamide A/C) (15), and T. erythraeum IMS101 (trichamide) (16) and from additional organisms.
Mass Spectrometry.
Sample preparation.
Recombinant MBP-McbA (110 μl at 40 μM) was proteolytically digested with 50 NIH units of thrombin (Sigma–Aldrich) for 4 h at room temperature in thrombin cleavage buffer: 50 mM Tris (pH 7.8) and 10 mM CaCl2. During the course of the reaction, cut McbA precipitates (pI ≈ 7) as a white solid. Precipitated McbA was subsequently harvested by centrifugation and washed two to three times with 100 μl of deionized water to remove the majority of buffer, salts, detergent, cut MBP, and uncut MBP-McbA. This precipitate was then resolubilized in 60% MeCN/2% formic acid (40 μl). Samples of MBP-McbA that were treated with BCD synthetase complex (Sag and Pag) were first reacted, as described in In Vitro Synthetase Assay, before thrombinolysis.
The MALDI target (stainless steel, Applied Biosystems) was prepared by allowing a saturated solution of sinapic acid (Sigma–Aldrich) in 50% MeCN/0.1% TFA to fully dry on the target (1.5 μl per sample, in duplicate). The precipitated McbA sample was then diluted (1:1) in the saturated matrix solution before spotting on top of the dried spot (1 μl in duplicate).
Instrument settings.
MALDI-TOF mass spectra were acquired on a Voyager DE-STR instrument (Applied Biosystems) in linear positive mode. Specific settings were typically as follows: 2,000–2,100 laser power, 93% grid, 0.12% guide wire, 400 nsec delay, mass window 3,000–18,000 Da.
In Vitro Synthetase Assay.
Synthetase reactions using MBP-tagged SagA and SagBCD and other fusion proteins were performed in a manner described earlier (27, 28). Reaction mixtures consisted of MBP-SagA (10 μM) and MBP-SagBCD (2 μM each) in a total volume of 100 μl of synthetase buffer (50 mM Tris·HCl, pH 7.5/125 mM NaCl/20 mM MgCl2/2 mM ATP/10 mM DTT). Reactions were allowed to proceed at 37°C for 16 h unless otherwise stated. These reactions were used for hemolytic assays and LDH release assays to quantify membrane damage. Omission of either ATP or DTT resulted in loss of hemolytic activity (data not shown).
Hemolytic Assay.
Fresh defibrinated whole sheep blood was obtained from Hemostat Laboratories. Whole blood was washed three times in PBS and diluted to a final concentration of 1:25 vol/vol in PBS. Prepared whole blood (100 μl) was then placed into individual wells of a flat-bottom 96-well microtiter plate (Costar, Corning). Reactions from in vitro synthetase assays were then added directly to the wells, and the mixture was reacted for 16 h at 37°C unless otherwise noted. After incubation, plates were centrifuged at 500 × g for 10 min and an aliquot of supernatant (100 μl) was placed in a separate microtiter plate for measuring hemoglobin absorbance at 450 nm on a Victor3 microplate reader (Perkin–Elmer).
We thank Joyce Limm for technical assistance and Christopher Walsh (Harvard Medical School, Boston) for the mcbA–D clones. We also thank Elizabeth Komives and Michael VanNieuwenhze for their helpful suggestions. Seema Mattoo critically reviewed the manuscript, for which we are grateful. This research was supported by grants from the National Institutes of Health, the Walther Cancer Institute, the Ellison Foundation, and the Howard Hughes Medical Institute (to J.E.D.). D.A.M. is supported by a Hartwell Foundation postdoctoral fellowship. S.W.L. is supported by a University of California at San Diego Heme and Blood Proteins postdoctoral training grant.
- **To whom correspondence should be addressed at: Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815-6789. E-mail: jedixon{at}ucsd.edu
Author contributions: S.W.L. and D.A.M. contributed equally to this work; S.W.L., D.A.M., M.E.H., P.C.D., and J.E.D. designed research; S.W.L., D.A.M., A.L.M., M.E.H., D.G., A.W., and P.C.D. performed research; S.W.L., D.A.M., A.L.M., and M.E.H. contributed new reagents/analytic tools; S.W.L., D.A.M., A.L.M., M.E.H., D.G., A.W., P.C.D., V.N., and J.E.D. analyzed data; and S.W.L., D.A.M., and J.E.D. wrote the paper.
The authors declare no conflict of interest.
See Commentary on page 5655.
This article contains supporting information online at www.pnas.org/cgi/content/full/0801338105/DCSupplemental.
- © 2008 by The National Academy of Sciences of the USA
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Watch Dr. Shepard's interview with Pete Wilson from The Telegram News
Click here for important information as of March 30th from Dr. Marci Shepard
Band Members Recognized
Oak Hill Band members were recognized by the Oak Hill Union Board of Education at the board meeting.
Solo and Ensemble
Twenty-nine Oak Hill Band and Choir students participated in Solo and Ensemble at Gallia Academy High School. Students performed for a judge and received a rating. Oak Hill students received 19 Superior Ratings (top rating), 9 Excellent Ratings (2nd rating) and 1 Festival Performance. Congratulations to these students!! February 15, 2020
Marching Oaks Place 1st
The Marching Oaks took the 1st place Marching Band trophy at the Beaver Oktoberfest Parade on Sunday, October 6, 2019.
Marching Oaks at Wellston
The Oak Hill Marching Oaks performed at the Wellston Ohio Hill Country Festival Hometown Parade on Wednesday, September 4, 2019. Click image to enlarge.


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Happy end of January! Crazy to think a month has gone by since we have rung in the New Year! February is such a short month, it will most likely go just as fast. The Super Bowl and Valentine's Day always have us on the edge of our seats to see all your crafty ideas and delicious recipes!
Hopefully the weather is a little bit better for most of you than last weekend! We did love seeing all your snow pictures, though.
Happy writing and commenting!
- Just Us Four Blog: Mini Chicken Parmesan Pockets
- Bread Booze Bacon: Peanut Butter & Jelly Pancakes
- Food Faith Fitness: Zucchini Noodles With Everything Pesto & Fried Eggs
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- Voice boks: 5 Simple Things You Can Do To Teach Your Kids About Money
- Pierced Wonderings: Misty Morning
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Link up your favorite post of the week!
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- The Greatest Showman Circus Inspired Costumes - Sep 12, 2019


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With a background from both the public and private sector, Christian has a long experience working with anti-financial crime. Starting out within the information technology sector, Christian soon joined the Swedish police force where he was working with financial crime cases as well as training and business development.
After several years within law enforcement, Christian moved to the private sector when he joined a large Nordic bank where he was part of building a strong team of financial crime experts. In 2018 Christian co-founded Acuminor, a regtech company helping both the private and public sector with financial crime risk management solutions
11/02/2020 / 17:00 - 17:20
With 400+ Fintech companies in Stockholm and an increasing amount of digital transactions, there has been a need for stepping out of the current 'isolated way of working'. As today communication is mostly limited to filling- and receiving- of police reports, more comprehensive cooperation between Fintechs and Law Enforcement might be a clear step forward. Despite a significant amount of goodwill, there have been organisational-limits on both sides. This panel focuses on potential future scenarios, current obstacles and pathways to possibly reach the goal to enhance the protection of millions of Fintech users. Every day.


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Poetry Station 4+
The English and Media Centre
- Free
This is the sister app to the award-winning web site The Poetry Station from The English and Media Centre. The English and Media Centre is a not-for-profit trust that provides publications and professional development on all aspects of English teaching for teachers and students of literature, language and media in the UK and abroad.
Watch and listen to videos of poets reading their poems often accompanied by animation, graphics and film. Save your favourites as you go to refer to later.
Visit www.poetrystation.org.uk for more details about the poets, their work and their publications.
NB This app requires a network connection to operate. A Wi-Fi connection is desirable for optimum video performance.
What's New
Version 1.2.1
Version 1.2.1
- complete refresh for iOS 10
- fixed bug in Topics categories
- iPad can be used in portrait or landscape orientation
- video can be viewed in either portrait or landscape orientation on any device
- several minor bugs and formatting issues fixed
Ratings and ReviewsSee All
My students love the Poetry Station website; the range of poets and performances are just incredible, and growing all the time.
This app takes the independent exploration of poetry to a whole other level! Whether it's creating the poetry cafe vibe of Kate Tempest's Cannibal Kids or the beautifully produced animations which accompany John Agard's readings - I can't wait to see which ones emerge as favourites now that they can all be accessed on iPhone and iPad.
A stunning resource!
Videos of poets reading and sometimes commenting on their own work. Actors reading some older poems. Very easy to navigate. Useful for teachers or A level and GCSE students. Still in its early stages I think and hopefully will have more poems in the future.
What a useful resource
Another excellent poetry resource from those good people at The English & Media Centre. This archive of materials should be invaluable for students aged 14-19 (and their teachers).
- Provider
- The English and Media Centre
- Size
- Category
- Education
- Compatibility
Requires iOS 8.0 or later. Compatible with iPhone, iPad and iPod touch.
- Languages
- Age Rating
- 4+
- Copyright
- © 2017 Created by The English & Media Centre. Poetry video copyrights are the property of their respective owners. App developed by Simon Buckingham, Icecandy Entertainment. All rights reserved.
- Price
- Free
Family Sharing
Up to six family members will be able to use this app with Family Sharing enabled.


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Function of the heart valves was discussed in Chapter 9, where it was pointed out that closing of the valves causes audible sounds. Ordinarily, no audible sounds occur when the valves open. In this chapter, we first discuss the factors that cause the sounds in the heart under normal and abnormal conditions. Then we discuss what happens in the overall circulatory system when valvular or congenital heart defects are present.
Was this article helpful?
This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.


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The Samba-Bugzilla – Bug 13737
Memory leak in lib/audit_logging
Last modified: 2019-02-05 14:13:14 UTC
Talloc context in audit_message_send is not created. So the memory is being allocated on the NULL context. Should not be an issue on production servers, as the message send code is only used for testing.
Created attachment 14758 [details]
Patch for 4.9
Pushed to autobuild-v4-9-test.
(In reply to Karolin Seeger from comment #2)
Pushed to v4-9-test.
Closing out bug report.


text:
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Servicios Personalizados
- Citado por SciELO
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- uBio
Mastozoología neotropical
versión impresa ISSN 0327-9383versión On-line ISSN 1666-0536
FASOLA, Laura; BELLO, Marcelo y GUICHON, M. Laura. Use of hair tubes and hair characterization of the Red-Bellied Squirrel Callosciurus erythraeus. Mastozool. neotrop. [online]. 2005, vol.12, n.1, pp.9-17. ISSN 0327-9383.
The Asiatic Red-Bellied Squirrel has been introduced into the Pampas Region, where a wild population has now been established and it is colonizing new areas. Hair tubes have been successfully used to assess the presence/absence of other species of squirrel through the identification of the collected hairs. The objectives of this study were to characterize the hairs of the Red-Bellied Squirrel and to test the use of hair tubes to detect the presence of this species. The study was conducted in the province of Buenos Aires, Argentina, from January to July 2004. Hairs from the back, head and tail have dark and light stripes while hairs from the belly are uniformly reddish brown. A multi-seriated medullar pattern was observed in the hairs of the back, head and belly, while the tail's hairs showed a different medullar pattern that can be described as overlapped dark rings. Cuticle scales patterns corresponded to the normal mosaic type in the hairs of the tail, back, head and belly, and for the last three regions a different pattern was observed next to the hair bulb. Hair tubes were made of PVC tubes (25x6 cm) opened at both extremes. The tubes had an adhesive tape attached at both extremes and bait (peanuts and nuts) was offered in the centre. Given the arboreal habits of this squirrel, traps were placed on tree branches and inspected weekly. The hair tubes were successful in collecting hairs of the Red-Bellied Squirrel that can be distinguished from those of other mammals inhabiting the same area. Because this is quite a simple and inexpensive technique, we consider it an appropriate method to evaluate the presence of this alien species in the region.
Palabras clave : Hair characterization; Hair tubes; Red-Bellied Squirrel; Sampling methods.


text: CRPRO: Protection Ritual Candle [CRPRO] $5.95 https://www.ravenandcrone.com/catalog/images/ritual_candle_protection_crpro_black_tn.jpg Protection Ritual Candle is an 8 inch black hand dipped ritual candle is charged, anointed, and inscribed with Theban script by a powerful witch to aid you in strengthening the magical and physical security you possess. Before you light the candle for the first time, focus your will entirely upon it, visualizing your intended strength and security being woven in and all around the candle. Let your mind fill with an awareness of the candle flame as a beacon, cutting through the darkness in your path. As the candle burns down to its base, keep your thoughts set upon the positive manifestations of attaining this blessed state of protection and awareness. You may choose to let the candle burn down completely at one time as it releases your ritually strengthened thoughts into the universe, or you can light and concentrate upon the candle 3 days in a row, making sure to snuff the candle at the end of each session, rather than blowing it out. Burning time 1 inch per hour up to a possible 7 hour burn time Quantity on hand : 66 Average Review Rating : 5.00 Current Reviews: 1 CRPRO: Protection Ritual Candle Reviews This product was added to our catalog on Friday 09 December, 2005. Quantity: We Also Recommend : APROTC: Protection Cross Amulet $8.95 Buy Now CPCPRT: Protection Reiki Charged Pillar Candle $12.95 Buy Now CVCSPROT: Protection Soy Votive Candle $8.95 Buy Now OPEAF: Peace n Protection Oil 2 dram $6.95 Buy Now OPROTC: Protection Oil 2 dram $6.95 Buy Now Protection Baby Wand Pendant $49.95 Buy Now Protection Gem Drop Amulet $33.95 Buy Now Protection Wand, Large $319.99 Buy Now RBPRT: Protection Bath Salts 5 oz $4.95 Buy Now Customers who bought this product also purchased HAGRC: Agrimony Cut 2oz $4.95 Buy Now Black Taper Candles 6 inch, 2 pack $3.50 Buy Now CS8069W: Brass Snuffer with Wood Handle $11.95 Buy Now Bloodstone Tumbled Stone Medium $1.15 Buy Now IGMYR: Myrrh Granular Incense $3.95 Buy Now HMANW: Mandrake Cut 1oz $5.95 Buy Now


text:
Petals & Peas
- Designer
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- Homewares
- Private Label
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- Living, Accents & Décor
Faux Floral Designer
Inspirational faux floral designs crafted by hand in my Cornish workshop. A passion for beautiful flowers transpires into some of the most realistic & enduring designs available
I am building a growing network of independent and high-end stockists in the UK & Europe and work with hotels,wedding & event organisers as well as larger organisations such as the National Trust.
My designs move with the seasons and are constantly evolving and developing as I explore new and exciting combinations of colours and styles.
2020 will see further exciting developments within my designs to incorporate dried and preserved stems as well as the beautiful high quality faux flowers i am recognised for.
With the new addition of gifts and stationary there is bound to be something beautiful to appeal.
AddressUnit 16
United Kingdom
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A variety of garlands for every occasion and every season Traditional and modern scandi styles Perfect for event hire, venue dressing, hotel decor and window dressing
Hydrangeas were my first love in the Petals journey and they have become a signature design in my portfolio. I will be showcasing a selection of designs using some of the most gorgeous faux hydrangeas ...
Timeless half wreath designs which suit contemporary and traditional settings. Romantic and luxurious, each design differs with the same overall effect. Availabel in pinks or limegreens/whites
A super sized luxuroius heart packed full of single petals and heads combined individually by hand to create the perfect blended effect At 60cm in dimater this an exclusive statement piece that will d ...
Soft pink rose and peonies blended with other pastel pink faux flowers to create a large 50cm statement piece for every occasion. Suitable for outdoor and indoor use.
A small and delicate design, using simple green and apple white hydrangea petals combined with delicate Queen Annes Lace Perfect for every season Approx 30cm in width
A selection of Christmas Designs for 2020 ranging from traditional classic reds and whites to modern Scandi style designs. Garlands, wreaths and natural twig bowers


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Extra $14.5bn spent on Labor states' bureaucracy splurge, by Adam Creighton.
Victoria and Queensland have supercharged their public sector workforces, hiring more than 100,000 new staff over the past five years at a cost to taxpayers of an extra $14.5bn a year in wages and salaries. …
That's $145,000 per year per public servant.
Public sector wages growth has exceeded that of the private sector for more than six years, according to the latest ABS data.
The bureaucratic part of the economy is doing very well, thank you.
Remember, bureaucrats have escaped the discipline of the market. They are paid what they think they are worth, not what the market thinks they're worth. Their wages comes from money taken by the threat of force (i.e. tax), not from money voluntarily exchanged for goods and services. There's a moral difference.


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Breast Self Exam (BSE)
The American Cancer Society recommends that all women over the age of 20 examine their breasts once a month. By examining your breasts regularly, you will know how your breasts normally feel. If a change should happen in your breasts, you will be able to identify it and let your doctor know. Most lumps are found by women themselves. If you find any lumps, thickenings or changes, tell your doctor right away. Remember, most breast lumps are not cancerous, but you don't know if you don't ask. Breast cancer may be successfully treated if you find it and treat it early. Delaying the diagnosis of breast cancer does not change the diagnosis, it only worsens the outcome.
When to do a Breast Self-Exam
You should do a Breast Self-Exam (BSE) every month 2 or 3 days after your period. If you do not have regular periods, just do it the same day every month...like the first... or the tenth...or the day that matches your birthday. (Note: just before your period or during pregnancy, your breasts may be somewhat lumpy or more tender.) If you are taking hormones, talk with your doctor about when to do BSE.
How to do Breast Self-Exam
1. Lie down. Flatten your right breast by placing a pillow under your right shoulder. Place your right arm behind your head.
2. Use the sensitive finger pads (where your fingerprints are, not the tips) of the middle three fingers on your left hand. Feel for lumps using a circular, rubbing motion in small, dime-sized circles without lifting the fingers. Powder, oil or lotion can be applied to the breast to make it easier for the fingers to glide over the surface and feel changes.
3. Press firmly enough to feel different breast tissues, using three different pressures. First, light pressure to just move the skin without jostling the tissue beneath, then medium pressure pressing midway into the tissue, and finally deep pressure to probe more deeply down to the ribs or to the point just short of discomfort..
4. Completely feel all of the breast and chest area up under your armpit, and up to the collarbone and all the way over to your shoulder to cover breast tissue that extends toward the shoulder.
5. Use the same pattern to feel every part of the breast tissue. Choose the method easiest for you:
Start in the underarm area and move your fingers downward little by little
until they are below the breast. Then move your fingers slightly toward the
middle, and slowly move back up. Go up and down until you cover the whole area.
Beginning at the outer edge of your breast, move your fingers slowly around
the breast in a circle. Move around the breast in smaller and smaller circles,
gradually working toward the nipple. Don't forget to check the underarm and
upper chest areas, too.
Starting at the outer edge of the breast, move your fingers toward the nipple
and back to the edge. Check your whole breast, covering one small wedge-shaped
section at a time. Be sure to check the underarm area and the upper chest.
6. After you have completely examined your right breast, then examine your left breast using the same method and your right hand, with a pillow under your left shoulder.
7. You may want to examine your breasts or do an extra exam while showering. It's easy to slide soapy hands over your skin, and to feel anything unusual.
8. You should also check your breasts in a mirror looking for any change in size or contour, dimpling of the skin or spontaneous nipple discharge.


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Director of Nursing (Westminster Village Aged Care Facility)
About you and the position
We are currently looking to appoint an enthusiastic Director of Nursing (DON) to manage our Westminster Aged Care Facility. The DON is accountable to the Senior Manager Aged Care for the effective and efficient management of all aspects of the Aged Care facility. Through sound leadership and management processes, the DON will ensure all residents receive quality care and enjoy quality of life appropriate to their individual needs and preferences.
Key responsibilities include:
To be successful in the role you will have:
About UnitingSAUnitingSA is a not-for-profit organisation that provides aged care, housing and community services to over 16,000 people across regional and metropolitan South Australia. With more than 1000 employees and volunteers, UnitingSA delivers high quality services to people from diverse backgrounds as we strive towards our vision of a compassionate, respectful and just community in which all people participate and flourish.
Culture and Benefits
A career with UnitingSA will offer you rewarding experiences to make a difference to the lives of people in need. We have an innovative and supportive culture guided by our values of respect, compassion, courage and integrity.
We ensure our staff are well supported, celebrated and compensated through:
To find out more about a career with UnitingSA, please visit our website at https://unitingsa.com.au/


text:
Your version of Microsoft Internet Explorer is not supported for secure online transactions. Would you like to update your IE browser?
Omni Hotels & Resorts offers guests online check-in. Save time at the front desk and check-in online 48 hours prior to arrival and we will have your room ready when you arrive. Simply pull up your reservation online up to 6 p.m. hotel time and confirm your billing details and you will be set.
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Named one of the "Top 121 Golf Resorts in the World" by Conde Nast Traveler.
Relax and revive at our full-service, nature-inspired spa.


text:
The Sunday Mail
The new political administration believes in an inclusive society that does not countenance racial discrimination, and is working with white former commercial farmers to see how they can contribute to the development of the country, President Emmerson Mnangagwa has said.
He also declared that the era of invading white-owned farms was over.
President Mnangagwa said white former commercial farmers who lost farms through land redistribution are free to take part in agricultural development through various contributions that can help the sector enhance productivity.
Addressing an interface meeting with members of the white community, Asians and those of mixed race in Harare yesterday, the Head of State and Government said no grouping is considered a minority community as Zimbabweans are one people.
"Under the new dispensation, there is no discrimination.
"We should cease to talk about who owns the farm in terms of colour. We should cease talking about that. A farmer – a black farmer, a white farmer – is a Zimbabwean farmer. We should look at it that way. We should begin to develop a culture among our people to accept that we are one."
President Mnangagwa said in line with his plans to restore Zimbabwe's status as the breadbasket of the region, Government welcomes white former commercial players who are willing to contribute their expertise to the development of the agriculture sector.
"Let the past remain behind, there are so many white farmers who don't have farms anymore as a result of land reform. But I think they have a role to play in terms of skills in the farming sector. All that is required is more of such interaction with the farmers' organisations. Invite me and I articulate my policy to the people, to the farmers so you find comfort and you know what is possible and what is not possible," he said.
"I told the farmers in Mashonaland West and in Chipinge and Chimanimani that this issue of new invasions is a thing of the past. I have told my provincial ministers, I have told my party leadership across the country that (this) is a thing of the past. The rule of law must now apply."
Government, he said, will also issue white farmers with 99-year leases.
"We want to restore the status of Zimbabwe as a food basket of the region.
"I met farmers in Mash West recently and I assured them that there will be increased pace in the issuance of the 99-year leases so that whoever owns a piece of land should feel secure and invest knowing that they have a 99-year lease in possession.
"But also, the 99-year lease is renewable and we want everything to be done to make it tradable.
"We have the rationalisation of land under the land commission (Zimbabwe Land Commission) that we instituted because during the process of land reform, many of us sitting here acquired more than one farm, pasi nazvo!
"We must correct that. Whoever has multiple farms, one for himself, one for the son and one for the grandchild, that land must be acquired.
"And we are saying many of the white commercial farmers who remained behind, who did not go away, we are very grateful for accepting this change and they must come on board and they must be issued with the 99-year leases wherever there are pieces of land which they hold," he said.
He also said the farm rationalisation exercise will apply to anyone, irrespective of rank.
"We are also downsizing farms and again, we are racially blind. It doesn't matter, if Chiwenga has a farm that is bigger than his area, we will downsize it, we forget that he is Vice President – he is a citizen like everybody else.
"The same with me and the same with everybody. We are going to make sure we do not have the animal farm mentality."
President Mnangagwa said Command Agriculture is voluntary and funded by the private sector.
Productivity, he added, is the ultimate cure for the black market.
"All that is needed is productivity and productivity, so that you reach a stage where there will be no need to deal with black market because it is not beneficial. There must be that commitment that the economy is ours together and it is only us who can move it forward. The issue now is business, business, business, wealth creation, and the politics will come only after five years but in between, we must make wealth."
After his speech, the President mixed and mingled with the crowd, which also had the opportunity to engage him and take "selfies".
The meeting was attended by Vice President Dr Constantino Chiwenga, Zanu-PF National Chairperson Cde Oppah Muchinguri-Kashiri and Minister of State for Harare Provincial Affairs Cde Miriam Chikukwa, among others.


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Simulation of aquatic locomotion
The discovery of water oceans under the icy crust of some Jovian and Saturnian moons has gave new perspective into how we seek for extra-terrestrial life. Several mission plan to assess the potential for life in the oceans of the icy-water moons, scan for bio-signatures and evaluate the presence of biologically essential elements. Moreover, these missions seek to map the surface and evaluate the ice crust thickness, opening the road for future robotic exploration of the ocean worlds. This undertake assumes great leaps in how we think about robotic exploration and several issues that need to be addressed: drilling into hundreds of meter thick ice, developing of autonomous aquatic robots, delivering power and enabling communication through the thick ice sheets are just some of them.
On Earth, a significant portion of the biosphere lives in the ocean. Through the evolutionary pressure, aquatic organisms have evolved into highly efficient swimmers in terms of energy consumption. Moreover, fish-schooling has proposed to reduce the energy expenditure even further. Investigating the aquatic locomotion evolved in Earth, can give guidelines into how to design efficient, low-weight soft robots and swarming behaviors that may be of advantageous for the exploration of the unknown environment of extra-terrestrial oceans.
Project overview
The current project looks into applying aquatic locomotion principles on the future robotic exploration missions in icy-water worlds. First, we seek novel ways to efficiently simulate the complex problem of aquatic locomotion in a simplified way. Then, we plan to employ computer simulations to better understand the key aspects of aquatic locomotion, in order to adapt them to the different conditions we expected to encounter in the icy-water moon oceans.


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Bloomberg sounded like The Gotham Gringo. Slick Willie (Bill Clinton) came off . . . unslick. Obama was polished, but less than authentic. George Bush sounded like a goofball, yet oddly authentic. Jeb Bush was impressive. Wanna know who really… Read More ›
Labor shortage now? Ha! Just wait.
I have an older brother. He's a tall, skinny redhead like me, except better looking. After graduating from Illinois State University in '99, he went to work for a home builder in Colorado. The builder had some… Read More ›
It's about retention, stupid.
"Do you know how much it costs our business to hire 800 people, so we can staff 400?" Unsure if it was a rhetorical question, yet certain my initial thought—"a lot"—was a poor response to verbalize to… Read More ›
Haunting Dreams en Español… one customer at a time.
Alcohol has its place in the creative process. Where, exactly, that is—well, that depends on the result. Sometimes you need a little distance from your new babe to determine if it's ugly or not. Even then,… Read More ›
Safety Spanish Spotlight :: Ladd Henley of Capform, Inc.
At Red Angle, we're always on the lookout for success stories – examples of English-speaking construction pros who learned Spanish to lead and manage more effectively. Ladd Henley is one such example. Upon learning Ladd… Read More ›
Whites: SNL's perspective on demography.
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Hispanics don't punt.
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With an expertise in employment law, Mark Papadopoulos has over 20 years of experience litigating cases and advising clients of various sizes on matters from the complex to the routine. Mr. Papadopoulos frequently provides advice and counsel to clients on matters such as internal complaint investigations, personnel policies, payroll compliance, employee disability and leave issues, and other human resources compliance matters. He presents seminars on the Fair Labor Standards Act, the Family and Medical Leave Act, and related wage and hour topics.
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ADDITIONAL VOLUNTARY MODULES (AVM's):
The standard has been designed to enable the addition of voluntary modules to the routine audit. Throughout the life of the standard new voluntary modules are developed by BRCGS. The voluntary module will allow the site to demonstrate compliance to specific sets of requirements in order to meet market or consumer requirements. The voluntary modules can be added to any of the full certification audit options (e.g. announced or unannounced).
List of current additional voluntary modules (AVM's:
• AVM 8 Traded Goods
• AVM 9 Management of Food Materials for Animal Feed
• AVM 10 Global G.A.P. Chain of Custody
• AVM 11 Meat Supply Chain Assurance
• AVM 12 AOECS Module for Gluten-Free Foods
• AVM 14 Food Safety Culture
• AVM 15 FSMA Preventive Controls Preparedness Module
From time to time BRCGS publishes position statements and guidance documents which may be binding to the certification audit process. NOTE: Position Statements are also published for Additional Voluntary Modules.
Issue 8 is the current standard. To download BRCGS standards and guidance documents go to the BRCGS Bookshop.
If you require assistance in developing or improving your management system for BRCGS Food Safety contact Ulrich today. He is a BRCGS Lead Auditor and an BRCGS Approved Consultant.


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Though using a CCTV security system can provide advantage in the form of catching all activity on video, its one major drawback is the limited visibility. The majority of the time, these cameras are only likely to capture certain portions of a room.
A wise home, both today and in the future, is just one where essentially all of the systems and devices in the home can join or talk to each other wirelessly, and can be centrally controlled either through a smart phone app or some type of voice recognition system.
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Looking for a security system could possibly be difficult things, the a variety of equipment, wireless and hard wired, cellular or phone line based, monitoring plans, contract terms – it might get messy. So how do you continue path, exactly how can you sort through the jumble and uncover what operates best for you and your loved ones?
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Normally the resident will receive a call from the alarm monitoring company to ascertain if it is a false alarm or learn whether the siren did its job of scaring the would be burglar away. Normally your telephone is wired directly to the alarm system firm. If so your telephones will close down allowing access solely to the alarm system company to call your home. A prearranged coded conversation usually occurs between the home occupant and the security company representative. After this code has been transmitted the "all clear" signal is given.
You can do a few things which are free or low-cost improve your home security. Believe it or not, one of the most effective methods is to put up a sign on your front lawn warning of a vicious guard dog. You don't even need it dog only a sign will do. Join or start a neighborhood watch program. They are quite effective, they're free and they work. Your local sheriffs department can allow you to get one moving.
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Budget always turns up, but you don't want to place it ahead of your security which may be thought of a penny wise and pound foolish, compare costs among businesses but hunt for a moderate package, the truth is that affordable and best almost never match, if you are going to cover your family's security make certain you are securing your money's worth. What are the features and benefits to youpersonally? Would a cellular system provide more peace of mind because it more safe and you don't need to purchase a telephone line. Do you desire a intelligent phone application and remote access to your own household? Customers with young children and teenagers have a tendency to buy monitoring programs with text message alarms, you could merely want a daily log in their comings and goings from your home. A security system is a good way to keep tabs on what's taking place in your property. Take these things into account whenever you're finding the very best home security company for the loved ones.
Most modern security alarm systems have the capacity to find a dial tone when sending a signal to the monitoring station. If a dial tone isn't detected, the panel assumes there's a call on the line so that it hangs up and tries again.
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Does anyone know about Roosevelt University?
I'd like to apply for the graduate study in Jounalism in Roosevelt University. I went to their page and found it has two campuses. One which is just a bulding is in Chicago, and the other one which has a campus is in Schaumburg.
Does anyone know if depatment Jounalism is in Chicago or Schaumburg?
Is this university good or bad?
How's it's ethnic status?
1 Answer
- lepkeLv 41 decade agoFavorite Answer
the "campus" in Schaumburg isn't what you think, it's just one, one story tall building, that's it, in the middle of the shopping district, and you would have to have a car to get there, Ikea is right down the street and about five times bigger
the downtown Chicago campus is three buildings, the Auditorium building 18 floors, the Gage building 12 floors, and the Herman Crown Center 19 floors, and all the journalism internships are in downtown Chicago, downtown campus also has furnished, dormitory-style housing
ethnic status? it's in Chicago, one of the most ethnically diverse cities you can find
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