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I plan to carry a brand new cat into my circle of relatives. She's an exquisite lady who has the very suitable title Bella (quick for Belladonna). She's specifically particular as a result of she has pussycat diabetes. She'll want ongoing care and a focus for the remainder of her lifestyles, however I've discovered that the fundamentals of maintaining a diabetic cat wholesome don't seem to be very frightening in any respect. Right here's what I've realized about diabetes in cats up to now:
1. Nutrition is a very powerful when coping with diabetes in cats
Diabetic cats shouldn't devour dry food. Maximum vets suggest a high-protein, low-carbohydrate weight loss program for diabetic cats, and no dry meals is low in carbohydrates. Even grain-free dry meals comprise numerous change carbohydrates comparable to potatoes, peas or tapioca. Carbohydrates generally tend to make blood sugar ranges differ rather a little. The safe haven the place I'm adopting my Bella has had cats that was diet-controlled and not wanted insulin after they started consuming low-carb meals. There are low-carbohydrate meals to be had at each and every value level, so that you don't have to shop for super-expensive meals to feed your diabetic cat correctly.
Involved in what people with diabetes should and shouldn't eat? Take a look at this text >>
2. House checking out isn't as arduous as it sort of feels
Like diabetic people, cats with pussycat diabetes want to have their blood glucose examined continuously. You'll be able to do that at domestic with a regular glucometer and checking out strips that you'll purchase in a drugstore. Report your cat's blood glucose stage, together with the date and time, after each and every check. The small vein working across the fringe of the ear is the perfect location to get a blood pattern for the check. Your vet can let you know how ceaselessly you will have to check your cat. You'll be able to to find step by step home-testing instructions and videos on-line to peer the way it's finished.
3. Giving insulin isn't as arduous as it sort of feels, both
In case your diabetic cat wishes insulin, it is important to give it by means of injection. The excellent news is that cats have numerous unfastened pores and skin between and round their shoulders, and that is the perfect location for giving photographs. Your vet will display you the way to try this, and movies of this process also are to be had on-line if you want a refresher.
4. You'll want to paintings intently together with your vet
Your cat will almost definitely want extra widespread examinations, specifically as her insulin dosage is being stabilized, and also you'll need to ship data of your cat's home-test blood glucose ranges for your vet so they may be able to turn into a part of your kitty's data. You'll additionally want prescriptions out of your vet to shop for your cat's insulin and syringes.
5. There are ongoing bills related to diabetes in cats
Taking good care of a cat with pussycat diabetes manner you'll want provides: syringes, check strips and batteries on your glucometer, insulin, and so forth. Puppy medical insurance doesn't duvet pre-existing stipulations, so in case your cat is diabetic whilst you undertake her, you'll want to be able financially if she has a well being disaster. A diabetic cat must be fed continuously — even supposing you're going away for simply an in a single day go back and forth, you want to make preparations on your cat to be fed and monitored. For the general public, that implies hiring a cat sitter. Be sure that sitter is ok with managing diabetes in cats and is aware of find out how to domestic check and administer insulin, too.
6. There's numerous beef up for pussycat diabetes to be had, too
The most efficient recognized and very best rated beef up web site fro diabetes in cats is FelineDiabetes.com. Dr. Lisa Pierson's CatInfo.org has a number of details about vitamin, in addition to a section dedicated to diabetes. Diabetic Cats in Need is a nonprofit that gives monetary help for caretakers of diabetic cats.
Diabetes in cats in point of fact isn't frightening. You wish to have to be skilled about pussycat diebetes, you want to have a vet you believe and with whom you'll be in contact smartly, and you want to be able for the truth that your way of life will alternate whilst you carry the sort of particular cats into your own home.
Let us know: Do you've gotten a diabetic cat? Are there assets and internet sites you've discovered specifically useful with regards to diabetes in cats? Tell us within the feedback!
This piece used to be at the start revealed in 2017.
Thumbnail: Images ©vladans | Thinkstock.
About JaneA Kelley: Punk-rock cat mother, science nerd, animal safe haven volunteer, skilled cat sitter, and all-around geek with a zeal for dangerous puns, clever dialog, and role-play journey video games. She gratefully and gracefully accepts her standing as leader cat slave for her circle of relatives of pussycat bloggers, who've been writing their award-winning cat recommendation weblog, Paws and Effect, since 2003.
Learn extra about cat well being on Catster.com:


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Vernal EquinoxThis evening, at 7:21pm EDT, the Sun crosses the celestial equator on it's trip north towards the Tropic of Cancer, marking the Vernal Equinox or first day of Spring. Today, the Sun rises due East and sets due West causing night and day to have equal (Equinox) lengths. Of course, in reality, this would only happen if there were no Atmosphere on Earth to scatter light, so the length of day and night visually differ slightly. From this point on, our days get longer by approximately two minuets per day. The Sun will stop it's Northern trek at the Tropic of Cancer, where we will have the longest day of the year in the Northern Hemisphere marking the Summer Solstice, than, start to head south again on the first day of Summer making the days shorter.
This may be more Astronomical then Ethnobotanical but everything is connected. As the length of day and the angle of insolation increases, the days get longer and warmer, triggering plants and animals to awaken and start there often ephemeral lives. In turn, we as humans, get more of the things we need to survive. Be Equal

subset: commoncrawl


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Sue Lovegrove: Vanishing #352
Sue Lovegrove Vanishing #352
Oil and resin on canvas 130 x 180cm
Sue Lovegrove was born in Adelaide, South Australia in 1962. She graduated from the ANU (Canberra) with a Bachelor of Arts (Visual) in 1990 and completed her PhD, also at ANU in 2002 with an analysis of Emily Kngwarreye's Big Yam Dreaming (1995). Her study of Kngwarreye's work helped develop her interest in 'between' spaces that lie beyond the surface of the painting. She discovered methods that can draw the viewer into the depths of pictorial space and then back to the surface.1 Based now in south eastern Tasmania, her practice has been crucially shaped by her engagement with the geography of Australia,1 interpreting geological, microbial and botanical forms and layers into beautiful and evocative works.2 She is a prominent artist and her work has been purchased by national public and corporate institutions such as Canberra Museum and Gallery, ANU, Parliament House Art Collection and National Gallery of Australia in Canberra and, the National Gallery of Victoria and Macquarie Bank, in Melbourne.2
Vanishing #352, procured by the University of Canberra in 2007, is one of a series painted as a result of the 2003 Australian Antarctic Division Fellowship and continues her practice of observing the myriad of layers in sea and ice landscapes.2 The series captures the ceaseless, abstract and fleeting sequence of ice formation, cracking, melting and refreezing with the ever present backdrop of stark white. Subtle semi-transparent textures of descending layers overlaid by fine line work and intricate detail describe the complex and endless depths of southern icy seascapes.
"I spent most of the journey on the bridge drawing and painting the ocean, the endless horizon or bergs passing like solitary white mountains lost in the ocean. It was a strange sensation steaming for days and weeks into the southern ocean waiting for the arrival of the ice.
Painting the ocean presented its own challenges: a multitude of layered patterns – small ripples and streaking on waves that sent plumes of spray in the air and then these made patterns on the bigger swells. And the exquisite range of greys: soft blue greys, grubby greys, creamy greys, dark indigo greys. At other times I painted from Terrascan satellite images – the ripples of cloud seen from the air in swirling lows and banks of cloud became brush marks that echoed the energy of the ocean and the ship's movement beautifully and in a way that the paintings never quite captured..."3


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Background: The increasing prevalence of psychological health problems among adolescent girls is alarming. Knowledge of beneficial effects of physical activity on psychological health is widespread. Dance is a popular formof exercise that could be a protective factor in preventing and treating symptoms of depression. The aim of thisstudy was to assess the cost-effectiveness of a dance intervention in addition to usual school health services foradolescent girls with internalizing problems, compared with usual school health services alone.
Methods: A cost-utility analysis from a societal perspective based on a randomized controlled intervention trial wasperformed. The setting was a city in central Sweden with a population of 130 000. A total of 112 adolescent girls, 13–18 years old, with internalizing problems participated in the study. They were randomly assigned to intervention (n =59) or control (n = 53) group. The intervention comprised dance twice weekly during eight months in addition to usualschool health services. Costs for the stakeholder of the intervention, treatment effect and healthcare costs wereconsidered. Gained quality-adjusted life-years (QALYs) were used to measure the effects. Quality of life was measuredwith the Health Utility Index Mark 3. Cost-effectiveness ratios were based on the changes in QALYs and net costs forthe intervention group compared with the control group. Likelihood of cost-effectiveness was calculated.
Results: At 20 months, quality of life had increased by 0.08 units more in the intervention group than in the controlgroup (P = .04), translating to 0.10 gained QALYs. The incremental cost-effectiveness ratio was USD $3,830 per QALYand the likelihood of cost-effectiveness was 95%.
Conclusions: Intervention with dance twice weekly in addition to usual school health services may be consideredcost-effective compared with usual school health services alone, for adolescent girls with internalizing problems.
Trial registration:
Name of the trial registry: "Influencing Adolescent Girls' With Creative Dance Twice Weekly"
Trial registration number: NCT01523561
Globally, psychological health problems are currently among the most serious public health challenges. Adolescent girls suffer from internalizing problems, such as somatic symptoms and mental health problems, at higher rates than in decades. By age 15, over 50 % of all girls experience multiple health complaints more than once a week and one in five girls reports fair or poor health.
The overall aim of this study was to investigate the effects of and experiences with an after-school dance intervention for adolescent girls with internalizing problems. The intervention comprised dance that focused on resources twice weekly for 8 months. Specifically, this thesis aimed to: I) investigate the effects on self-rated health (SRH), adherence and over-all experience; II) evaluate the effects on somatic symptoms, emotional distress and use of medication; III) explore the experiences of those participating in the intervention; and IV) assess the cost-effectiveness.
A total of 112 girls aged 13 to 18 years were included in a randomized controlled trial. The dance intervention group comprised 59 girls, and the control group 53. In paper I, the dance group showed increased SRH scores compared to the control group (p = .02). Girls in the intervention group showed high adherence and a positive overall experience. In paper II, the dance group exhibited a decrease in somatic symptoms (p = .021), emotional distress (p = .023) and use of medication (p = .020) compared to the control group. In paper III, a strategic sample of 24 girls was interviewed. Qualitative content analysis was performed, and five generic categories emerged. Two were "An Oasis from Stress" and "Supportive Togetherness", which was shown to represent the fundamental basis and setting of the intervention. The main category, participants' central experience, was understood as "Finding embodied self-trust that opens new doors". Paper IV revealed that, due to decreased number of visits to the school nurse and an increase in health related quality of life; the intervention was considered to be cost-effective (combined with the usual school health services). In summary, the results of this thesis show that this dance intervention for adolescent girls with internalizing problems generated positive health effects and proved to be cost-effective. For this target group, a non-judgmental environment and supportive togetherness proved to be of importance for participation. The results of this study may provide practical information for school health care staff and caregivers in designing future interventions.


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I was diagnosed with relapsing-remitting MS at 19, and for 26 years suffered the on-again/off-again awful symptoms of numbness, tingling, optic neuritis and profound fatigue. I had no idea that my chronic, mild digestive discomfort had anything to do with the MS. I just thought that it was normal to feel some digestive distress after meals.
In the "dark ages" of the 1980s, before the Internet opened vast horizons of information, there were very few books on healing MS. I believed that what I ate might make a difference, and so I experimented with macrobiotic, vegetarian and low-fat food plans. I even tried the Swank MS Diet, an ultra low-fat approach. Nothing helped. In fact, the lower the fats and higher the grains, the worse I felt.
Finally, by 2010, I had educated myself enough to know that I needed help in finding the root causes of my MS. I decided to see a functional medicine nutritionist. Test results revealed that I had non-celiac gluten sensitivity (NCGS) and a "leaky gut."
The nutritionist advised me to eliminate gluten from my diet and led me through a 30-day gut-healing protocol. From that day forward I went cold turkey on gluten. Within a week I stopped experiencing tummy issues following meals, and within a few weeks, the numbness and tingling retreated. I have never again experienced a single MS symptom. A blood test later confirmed that my antibody levels against myelin sheath were completely within the normal range. That meant I no longer had MS!
In the intervening years, I quit my job as a sales and marketing executive to research how it was possible that I reversed an autoimmune condition. After all, each of my six neurologists over 26 years had told me "there's nothing you can do" – except take powerful immune-suppressing steroids or injectable medication. Yet, after reading hundreds of publically available, scientific studies, and dozens of books, including Grain Brain, Brain Maker, The Wahls Protocol, and The Immune System Recovery Plan, I learned there is much that I and anyone else can do.
I learned that health and disease is a continuum, and that environmental factors are mostly responsible for which end of the continuum we tilt towards. I found that the big categories come down to food, infections, gut health, hormone balance, toxins and stress, which happen to spell the helpful mnemonic, F.I.G.H.T.S.! And of these categories, integrative physicians and immunologists agree, food is the best place to start. I also learned about epigenetics – the relatively new and very exciting science that reveals that we can modify how our genes get expressed.
Today I follow a modified ketogenic diet, which means I avoid all grains and instead opt for a rainbow of above-ground vegetables, grass-fed meat and wild-caught fish, and ample healthy fats, like coconut and MCT oil, avocado, and raw, sprouted nuts and seeds. The bonus of eating this way is that, at age 51, I'm in better health and fitter than ever before.
I decided to devote my life to helping those seeking to reverse or prevent autoimmune conditions become empowered and get the information they need to live their most authentic and vibrant lives. I blog and post daily about the heart and science of getting and staying well. I am also studying to be certified as a Functional Medicine Health Coach.
A huge THANK YOU to Dr. Perlmutter for going against the grain of last century's neurology, and for educating and empowering the world to get and keep our brains and bodies in the best shape possible!


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ur last issue caused a bit of a stir, as the diversity of letters below indicates. It should come as no surprise that not every humanist believes the same things about the state of the world today, and that's a good thing. It reminds us of our reflexive distaste for rigid dogmatism, and that we value the thoughtful process by which we arrive at our conclusions as much – if not more so – than the conclusions themselves. May this issue provide the reader with more grist for their mill.
– Richard Young
Brava for your intrepid and accurate aria about the new totalitarianism [HP202]. What you analyze so sagely is also heartbreaking.
I wanted to commend you for including the article in the Autumn Issue (202) 2017 by Vir Narain, "Rethinking Our Objectives and Strategies." I applaud Vir's comments with regard to the relationship needed between the religious individual and humanism. He notes that:
... this might involve a reorientation of the Humanist attitude towards supernaturalism and theism. ... There are indications that, in rejecting religion altogether, we are also denying that there are any 'religious implications of our ideas'. ... The best that the Humanist Movement can do in this situation is to strengthen its position in the West. For this it has to assume the role of a successor, not an enemy, (emphasis mine) of religion.
I would like to quote from the book, "The Ethical Imagination, Journeys of the Human Spirit", by Margaret Somerville: "Toward Finding our True, Ethical, Human Selves Through a Shared Ethics: Whether we frame our quest for a shared ethics in a search for truth or as a search for our true ethical human selves might not matter in terms of results, but the latter is likely to appeal to more people than the former. Still, no matter how we frame our quest, we must decide what we understand by 'truth' in the context of a shared ethics. For instance, to say either that God does or does not exist is not 'true,' in the sense that such statements can neither be proved nor disproved scientifically. Nor can we all agree on one position or the other as a fact. Therefore, neither view can define for all of us what we mean by our true, ethical human selves nor found the shared base on which we can establish values we hold in common. If we are going to find a shared ethical base, that base must be able to accommodate both those who believe in God and those who do not – in short, it must not be antithetical to either. This requires that we translate our values, whatever their base, into a language we can all share. ... Here I merely want to emphasize that religious people must try to speak their truth in secular language and non-religious people must avoid speaking their truths in anti-religious language, if we are to engage in constructive dialogue."
Perhaps something worth considering.
Thank you for your choice of meaningful articles. Much appreciated.
(Note: In my own thinking I take the stance of an agnostic ... a cowardly view some might say, however, it accurately reflects my immediate state of mind. My field of study is the Psychology of Religion. I was ordained to ministry in an interdenominational setting where the thrust of my sharing was on the "practical" and not the "dogmatic" role of religion in one's life. Hence, my inclination to like the view taken by Margaret Somerville.)
Sophie Dulesh's article "The Regressive Left and Dialectics" [HP202], although containing a few valid points, was misguided in its duplicitous Fox News style attack on Noam Chomsky, falsely claiming that he "defended Pol Pot". This assertion is ludicrous.
The disgraceful smear campaign launched against Chomsky by Humanist Perspectives, like so many others over the years by many neo-conservative and neo-liberals lap poodles, is unwarranted.
Since the 1970s Noam Chomsky has courageously challenged American hegemony, imperialism, illegal and immoral wars, horrific atrocities and terrorism which have resulted in countless attacks on him from the sycophantic right wing corporate media and intelligentsia. All have been systematically refuted multiple times. But those who challenge power are expected to be vilified; of this Chomsky is well aware. He has admitted that if he wasn't the target of vitriol from the capitalist state and their corporate media echo chambers, he'd be doing something wrong.
Noam Chomsky's integrity is unparalleled.
I direct you to an article titled "The Boring Truth about Noam Chomsky: He Does Not Support Pol Pot" by Michael Brull. [https://goo.gl/V3PCBE] Also consider a letter to The New York Times regarding this issue by the late Ed Herman [https://goo.gl/q93Lh2].
I also take exception to Dulesh's inclusion of Chris Hedges, Glen Greenwald, Jeremy Corbyn, Cenk Uygur and others as members of the oxymoronic Regressive Left. The descent of the left into identity politics is regrettable but to refer to those dissenters such as Noam Chomsky and others as "regressive" seems quaint at best.
Isn't regressive a term generally reserved for calcified conservatives and reactionaries like Ronald Reagan, Bush I and II, Stephen Harper, David Cameron and Donald Trump?
Oddly, Dulesh in her hit list of left wing "regressives" has left out another of my respected leftist intellectuals, Michael Parenti.
I believe you have hit the nail on the head in your recent editorial ("Social Justice – the new totalitarianism?"), and as a result you will be getting the usual denials and attacks. I hope you persist and expand on this topic.
In the last four months, I have woken from my slumber.
Based on who is invited to speak at our local Humanist association, I wonder if Humanism is no longer for skeptics and freethinkers but is instead becoming a venue for the ideology of Critical Theory, an ideology sometimes referred to as Cultural Marxism, which opposes free speech under the guise of not hurting anyone's feelings. On the YouTube channel The Rubin Report, ex-Muslim Sarah Haider has stated that the political Right is far more accepting of ex-Muslims than the Left, possibly because they do not fit the political Left's narrative. How things have changed!
Based on my personal observations at our meetings, I have serious concerns about the direction that this movement is taking. At one meeting, some "social justice warriors" of Indian and Pakistani background defended the niqab, and one mentioned friends in the Antifa movement. So why are face coverings important? When I mentioned Lindsay Shepherd at another meeting, a couple of attendees looked annoyed. A young volunteer at a crisis center gave a talk concerning sexual violence only to slip into political rhetoric that "we live in rape culture," and "colonization is the problem"! Challenged by the humanists, she appeared unable to extract herself from the political narrative, repeating "we live in a rape culture" and blaming everything on "colonization."
This leaves me concerned about our education system. Are our institutions of learning little more than indoctrination centers designed to silence critical thought?
Rather than being a beacon of freethought and critical thinking, I fear that Humanism is becoming just another avenue to advance the agenda of the cultural-Marxist social justice warriors.


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Hydrothermal vents
"What are some geographic anomalies near underwater volcanoes?"
Underwater volcanoes are quite fascinating structures, but does the presence of these mechanisms cause the creation of other alien-like structures? Well, it turns out to be true. If you were to look at underwater volcanoes near spreading ridges and convergent plate boundaries, then you will find a very interesting series of structures known as hydrothermal vents. Hydrothermal vents are fissures in a planet's surface that are produced when hot magma from the underwater volcanoes meet the near freezing seawater and reemerges from the surface. The seawater produced by hydrothermal vents can reach temperatures of over 700 degrees Fahrenheit! These unique conditions of hydrothermal vents are actually the living space for many communities of extremophiles. Hydrothermal vents are actually a unique discovery, being found only less than 40 years ago.


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Year : 2013
Style : Technical Death Metal
Country : Canada
Audio : 320 kbps + all scans
Size : 163 mb
Luc Lemay resurrected Gorguts under the advice of Negativa bandmate Steeve Hurdle, when it was decided that Negativa would move in a different direction, rather than being a continuation of the Gorguts sound. An instrumental demo of one of the new songs, consisting of Lemay playing the rhythm guitar part over a click track was leaked onto the internet in December of 2008, after which Lemay confirmed that Gorguts had indeed been reformed.Drummer Steve MacDonald reportedly hung himself sometime during the 3rd week of October 2002 after battling depression the last few years.Luc Lemay joined Negativa with Steeve Hurdle after Gorguts disbanded in 2005, but felt uncomfortable with the improvisational elements in that band's music.At Hurdle's recommendation, Lemay reformed Gorguts in summer 2008 in order to prepare for the twentieth anniversary of Gorguts' 1989 formation.As Lemay recollected, "When Steeve brought the idea to me to make a new record to celebrate the 20th anniversary of the band, I'd never thought about it before. I was very happy with everything the band had accomplished in the past and I had no intention of making a new record. Then, when I started to write, I had no apprehensions either...I just went with the flow and wrote the music I wanted to hear".
Colored Sands is the fifth full-length album by technical death metal band Gorguts. It is Gorguts' first studio album since 2001's From Wisdom to Hate. The album features the band's first recordings with guitarist Kevin Hufnagel and bassist Colin Marston, and the band's only recordings with drummer John Longstreth. It is a concept album based on Tibet.[2] The album was released digitally on August 6, 2013, and the release of the CD and vinyl versions on September 3, 2013.A rough instrumental demo of "Enemies of Compassion" was previewed on the band's Myspace page in 2011.Prior to the album's release, the songs "Forgotten Arrows" and "An Ocean of Wisdom" were made available for online streaming. As a response to the album leaking onto file sharing sites in July 2013, Season of Mist released a digital version of the album on August 6, nearly a month ahead of the scheduled release date.The CD and vinyl versions were released on September 3, 2013.
Line Up:
Luc Lemay Guitars, Vocals (1989-2005, 2008-present) - See also: ex-Negativa
Colin Marston Bass (2009-present) - See also: Behold the Arctopus, Byla, Dysrhythmia, Indricothere, Krallice, Colin Marston, Sailors With Wax Wings, ex-Infidel?/Castro!
John Longstreth Drums (2009-present) - See also: Dim Mak, Origin, ex-Malicious Intent, ex-Possession, ex-Skinless, ex-The Red Chord, ex-Dying Fetus (live), ex-Exhumed (live), ex-Angelcorpse
Kevin Hufnagel Guitars (2009-present) - See also: Byla, Dysrhythmia, Vaura, ex-While Heaven Wept, ex-Euclid Street, ex-Grey Division Blue, ex-The Fifth Season
01. Le Toit du Monde 06:33
02. An Ocean of Wisdom 07:20
03. Forgotten Arrows 05:42
04. Colored Sands 07:55
05. The Battle of Chamdo 04:42 instrumental
06. Enemies of Compassion 07:03
07. Ember's Voice 06:48
08. Absconders 09:08
09. Reduced to Silence 07:38
Please my link you can´t spread further and don´t upload to other hostings!!!
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Suicide attackers have targeted two natural gas facilities in the central Syrian province of Homs, killing eight people, officials said.
State news agency Sana also reported the attacks, saying guards opened fire at the attackers as they drove towards the plants, forcing them to detonate their bombs before reaching their targets.
A local official in Homs said the attacks killed eight people and wounded 12. There was no claim of responsibility but opposition fighters and jihadis have frequently targeted the country's oil and gas infrastructure during the near four-year conflict.
Sana said the facilities are operating as usual after the attacks.
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Carol Lee Beloher
Carol Lee Beloher (Jasper, New York, USA)
Carol Lee Beloher Poems
Comments about Carol Lee Beloher
Best Poem of Carol Lee Beloher
Tell Me! Who?
Who barricaded the old with doors, which began
to go forth as when it burst out from the womb, how
about the cloud in it's garment or thick gloom us
it's swaddling band, and to proceed to break up regulation
upon it and to set a bar and doors and say this for you
may come and no farther and here the works are limited,
who caused the down to know it's place?
to take hold on the extremites of the earth? Where now
is the way by which the light distributes itself?
Have you entered into the storehouse of the snow?
do you see even the storehouse of the hoil?...


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Agra which is known as the city of Taj Mahal is also famous for plenty of other benchmarks which have supported the development of the city.
The medical facilities available in Agra have also given new scope of developments to the country.
In the list of the cities recognised medical institutions one of the name that stands well acknowledged is Lotus Super Speciality Hospital which is located in the prime location of the city at Diwani Chowraha.
Lotus Super Speciality Hospital provides all the new medical amenities and health related services for their patients and promises to keep its standards lifelong.
We have extremely qualified Doctors and Specialists who work with full dedication and put forward their entire efforts to bring a smile on the face of our patients and their family.
The hospital is equipped with 80 Beds and all kind of new machines and apparatus which are used for various treatments .These qualities makes the hospital well known not only in the city but also in its suburbs.
The entire team of Lotus Super Speciality Hospital works with full dedication to provide their patients all kinds' medical facilities and amenities.


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Please call us at: (989) 798-2711 or fill out our contact form.
Door of Hope
1518 Hiawatha Dr
Owosso, MI 48867
The Next Campaign to raise funds.
Those affected by Cyclone Idai March 18, 2019
and Shiawassee county families effected by tornado March 14, 2019 to donate time, talent or finaces, please contact us.
Connect with us on Facebook & Twitter to learn about upcoming projects and volunteer opportunities.
Door of Hope was founded on the idea of helping those in need. Ever since our organization was established, we have endeavored to provide help where the need is greatest.
Our team is made up entirely of volunteers. We advocate for those who need our help. Even a little help goes a long way. As mother Theresa states, "Small things done in great LOVE will change the world"
That is why we are especially happy to find new volunteers who share our commitment and passion to doing good in the world. Do you want to support us by helping with our work? Then do not hesitate to contact us. A helping hand is always greatly appreciated!
Cynthia Merling President / C.E.O.
E-mail: firstname.lastname@example.com
Phone: 989- 798-2711
Cindy has held numerous management and leadership positions ranging from missions director to small business owner. She has vast experience in the area of coordination and implementation of humanitarian and missions related travel around the world. It was while on a trip to Africa that Cindy was moved by the deplorable water conditions to start a LLC known as "Coffee for A Cause" and eventually to the creation of Door of Hope.


text:
The CMO's Guide To Investing In Product Advertising-
How does your retail strategy stack up? This guide gives you everything you need to take it to the next level…DOWNLOAD WHITE PAPER
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Honda Civic – 2004 is a seventh generation model, it is 2000 launched model with upgraded bumpers, front grille , tail lamps, eco driving indicator, alloy wheels and front airbags. It has several different variants with the changes in vehicle's dimensions. Similar changes are also made by Honda Philippines with additional interior design change.
Honda equips its vehicles with some of the most comfortable and mesmerizing interiors, which make you feel luxurious and elegant while driving or sitting. Starting from the dashboard which is sleek design made up of plastic, leather and rexine same as the seats, in the middle you would find the ac air output, multimedia system, ac controllers and small container to put tiny stuff. While in the middle there is a manual or automatic gear lever, a hand break and a box to put medium size stuff. At the other side of the steering wheel there is glove compartment.
The engine of Honda Civic – 2004 has different types of variants engines which offer both manual and automatic transmissions in 5-speeds. It has Honda K-series engine with DX, LX, EX, and HX variants too with i-VTEC motor technology.
Honda Civic 2004 is estimated 11 kmpl to 12 kilometers per liters in city and up to 14 kilometer per liter on highways, which is best in 1600 cc car mileage in comparison to other giants of brands. Also hybrid variant save more fuel with 1.3-liter engine.
It offers most attractive colors to its customers including Galapagos Green Metallic, Magnesium Metallic, Nighthawk Black Pearl, Opal Silver Blue Metallic, Eternal Blue Pearl, Fiji Blue Pearl, Opal Silver Blue Metallic, Radiant Ruby Pearl, Rallye Red, Satin Silver Metallic, Shoreline Mist Metallic, Taffeta White, Vivid Blue Pearl and more.
Check out all variants of Honda City – 2004 with details specifications, all variants and high quality images of interior exterior and prominent features with variety of colors at shoprex.com, also the updated price of each model is also listed with the respect of its variation and type.
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Book Review: A Kiss from Mr Fitzgerald by Natasha Lester
Evie is a woman after my own heart, chasing her dreams and doing whatever she can to make them come true. She also happens to fall in love with a man she doesn't expect to, and it's not love at first sight either. I enjoyed the fact that Evie had to work a second job in order to pay for her medical studies and her life. Thomas is the perfect man for her, and it's easy for the reader to fall in love with him too. In fact, each character is brilliantly written, and there are so many who give the world a real-life feel.


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This Hot Holiday Toys Round-Up + Giveaway might give you some gift giving inspiration for the boys and girls on your list. Or family game night. Or vacation, screen-free, boredom buster! All of these games offer opportunities for players to enjoy hands-on fun. Read on for what made the list for the Parent Club Hot Holiday Toys Round-Up…
Note: the following giveaway is open to residents of Canada, excluding Quebec.
MGA Entertainment
Social Star: players must climb the social ladder and gather 'Likes' in order to gain fame. For: 3-8 players
Includes: 100 challenge cards, 100 like cards, 50 dare cards. Age Recommendation: 13+.
Ready2Robot Battle Pack: build, mix and match custom 'bots, and battle your friends in a robot-brawl. Includes: 2 Mechbots, 2 Pilots and weapons to build battles. Age Recommendation: 5+.
Air Chargers Twisted Turn Crashway: The easy to use pump launcher allows kids to quickly charge up their race car and go! Launcher allows you to be in control of the speed, distance and crashing! Customize the distance and height of your barricade. Age Recommendations: 5+.
Crate Creatures Assortment: Crate Creatures are fun interactive pets that you unleash from their cage to
find out which special features they have. Break into the crate to unleash your new beast friend. All you need to do is use the crowbar to pop the lock. Pull their tongue for monstrosly fun and gross noises and watch as the creature
vibrates and it's eyes start glowing. 45 sounds. Age Recommendation: 4+
Retail Availability: Toys "R" Us, Walmart and Indigo
Mattel Games
Gas Out: Players take turns playing Gas Out Number cards in their hand and pressing Guster as many times as
shown. Be the last player to keep Guster the Gas Cloud from farting during your turn, and you win! Age Recommendation: 5+.
Squawk: Roll the dice and then press on the chicken's chest the number of times shown on the dice. You'll hear a funny "SQUAWK" – if she doesn't lay an egg, then it is the next player'sturn, but if the hen house opens, she'll lay an egg that you get to crack open. Press your luck as you try to collect the most baby chicks. Age Recommendation: 5+.
Retail Availability: Canadian Tire, Toys "R" Us, Amazon
Kerplunk: (I remember playing this game as a kid!) The object of the game is to skillfully remove the sticks from the tube while dropping the fewest marbles possible. You and your friends will have a blast playing Kerplunk, just don't let the marbles fall! Age recommendation: 5+.
Retail Availability: Canadian Tire, Real Canadian Superstore, Toys "R" Us, Walmart, Amazon
Robotic Soccer: Designed to mimic real-life soccer, this toy's play pattern taps into the love for soccer and pairs it with the education fun of robotics. Pick a player, customize it with decals and send it on to the field to kick balls into goals. Age recommendation: 8+.
Toys and games are a great gift idea for Secret Santa too. My friends and I are planning a Secret Santa night right now. The rules are: bring an non-gendered, wrapped gift (and all of these games fall into both those categories). And yes, I have my gift giving inspiration done from this post!
Disclosure: #giftedproduct. All opinions are my own.
Enter for a chance to win a prize pack of toys!


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I have done lots of computer work over the last few weeks and decided it was time to sew some outfits.
I started off with the idea to make a 3/4 sleeve T and ended up trying a few new things. I have had a flounce pattern hanging around for a while. I made a cute skirt with it a long tome ago. This time I have put it on the sleeves of the top. It is all art of experimenting. I think I will make a new flounce pattern with a two circle spiral to get a different look. My original is just a circle with a hole in the middle. It is a perfect fit for joining 2 across a front or 3 around a v neck or 4 all around a top.
The neckline is cut out just at the shoulders, not at the front, so it looks a bit wider than round. Very easy to do. I just folded it at the shoulder seam and trimmed a bit off.
I wanted to make a new vest and skirt and few other garments from the different views in 1805 to celebrate the updated pattern printable.
- I have added the shading for the right side of the fabric in the diagrams.
- I have spaced it out a bit more and enlarged the heading to make it easier to read.
- I have enlarged the pattern envelope so you can fit an A5 booklet in it.
- I have divided the pattern into 2 files - one for the pattern pieces and extras to be printed at 100% and one for the Pictorial Instructions so they can be printed either as A4/US Letter or as a booklet to be folded in half and stored in the envelope.
|Booklet, Envelope and Filing Divider page
To make a booklet all you have to do is open the Pictorial file in Adobe Reader, choose booklet and print. Most printers print double sided these days so it is really easy. Use two or three staples close to the fold to hold it all together.
You can buy 1805 in my Etsy shop for my basic pattern price.
Happy Sewing,


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Well, after her Gully Boy co-star Ranveer Singh took the internet by a storm with his dance at his bestie's wedding, it is time for Alia Bhatt to set the social media on fire! No, she ain't grooving to any of the chartbusters from her film as yet but we are talking about the bridesmaid avatar that she has donned for her friend's wedding. The actress seems to be getting quite rave reviews for her bridesmaid look she posted on Instagram.
Alia Bhatt shared some intriguing Instagram stories on the platform wherein we get to see the Gully Boy actress in a traditional avatar. While those trendy jhumkas are an apt match for her lehenga avatar, her simple makeup acts as the perfect add-on. The actress shared a boomerang video of her playing with black shades and also a photo with the other bridesmaids on the social media platform. Fan clubs too seem to be quite impressed with the actress' looks and have been sharing the same on their respective pages.
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This is not the first time we got a glimpse of Alia Bhatt as the bridesmaid. The actress has been a bridesmaid for her friends in the past too and has even performed on their sangeet. Coming to her marriage, the Bhatt girl recently rubbished the marriage rumours with her beau Ranbir Kapoor. While the actress had confessed that she is in a happy space in the relationship, she spent her Valentine's Day with her Brahmastra co-star this year.
On the work front, Alia Bhatt is riding high on the success of Gully Boy. The actress will next feature with Varun Dhawan in the multi-starrer Kalank, starring Madhuri Dixit, Sanjay Dutt, Sonakshi Sinha and Aditya Roy Kapur. She will also be featuring in yet another multi-starrer Takht with Ranveer Singh, Kareena Kapoor Khan and others as well as in the superhero drama Brahmastra and her father's ambitious film Sadak 2.
Also Read: Woah! Katrina Kaif is impressed with Alia Bhatt's performance in Gully Boy
Catch us for latest Bollywood News, Bollywood Movies update, Box office collection, New Movies Release & upcoming movies info only on Bollywood Hungama.


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Heat Transfer & Phase Change Blog Posts
Introducing the Metal Processing Module
The Metal Processing Module includes 2 interfaces for analyzing diffusive and displacive metallurgical phase transformations: Metal Phase Transformation and Austenite Decomposition.
Heat Transfer in Biological Tissue with Thermal Damage Analysis
Hepatic tumor ablation using radiofrequency heating, microwave coagulation therapy, and other cancer treatments can be studied by modeling the electromagnetic heating of biological tissues.
Finding the Best Way to Make Crêpes with Fluid Dynamics Research
The recipe for the perfect crêpe calls for flour, eggs, milk, sugar, butter, and…simulation? Fluid dynamicists used simulation to solve the optimal coverage problem for crêpe making.
The Importance of Thermal Radiation in Your Models
Conduction and convection tend to get more attention from simulation engineers, but radiation plays an important role as well. Learn how (and why) to account for thermal radiation in your models.
4 Methods to Account for Radiation in Participating Media
The discrete ordinates method, P1 approximation, Rosseland approximation, or the Beer–Lambert law: Which should you use to analyze heat transfer in participating media?
Heat Transfer with Radiation in Participating Media and the Discrete Ordinates Method
Here's your complete guide to the discrete ordinates method, quadrature sets, and how to model the interaction between radiation and a participating or absorbing medium.
Comparing Optimization Methods for a Heat Sink Design for 3D Printing
When designing a heat sink, is it better to use parametric or topology optimization? When manufacturing said design, does 3D printing produce the best result, or a conventional method?
Calculating the Heat Transfer Coefficient for Flat and Corrugated Plates
What is the heat transfer coefficient, and how do you calculate it? This blog post includes a theoretical background and demonstration of 2 examples in COMSOL Multiphysics®.
- Chemical
- COMSOL Now
- Electrical
- Fluid
- General
- Interfacing
- Mechanical
- Today in Science


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Mother and daughter vacations are always a good idea! Whether you want to spoil your mother for providing you such a great life or you want to make memories with your daughter before she grows up and starts a life of her own, mother and daughter trips are excellent excuses to escape everyday life and enjoy time together. AurTravel has a few ideas on where to travel for your mother and daughter vacation.
NYC: A trip to New Yok City is an excellent choice for mother daughter duos because it is such a diverse area with many different things to do for all ages and personalities! You can take a short weekend getaway or you can stay for a week. There is shopping, museums, parks, fine dining, tourist attractions, spas, multiple different cultural areas to explore, and so many more options. You can either take a tour or decide to venture out on your own. Catch up on life while walking through beautiful Central Park, see a world famous Broadway production, enjoy some retail therapy, or walk the streets and soak up all the beautiful architecture and culture. A trip to NYC for you and your mom is perfect because it allows for you to make amazing new memories with one of your favorite people in the world!
Cruise: Cruises are another great option because they are customizable and packed full of possible activities and excursions. Some choose to spend the whole trip relaxing by the pool; others choose to take exciting adventures at each port! We have booked many mother/daughter cruise vacations that people have absolutely loved; the environment is perfect for strengthening your bond.
Spa: If you both have busy lives and need a chance to take a break from it all, than a vacation to a spa resort might just be the perfect choice! There are many spa resorts around the country that a mom and daughter pair can enjoy. Sit back and relax while you are tended to and pampered. Enjoy the cuisine, facials, pedicures, and massages at your serene destination. At most spa resorts you don't even have to leave the property to have a fun, relaxing trip because everything you want will already be on property.
Bahamas: This destination is great if you want both relaxation and some adventure! And if a tropical white sandy ambiance is what you're looking for than this is definitely the place to go. With world class resorts, bright sunny sky, clear blue waters, and a paradise-like environment, you are sure to have a blast on your mother –daughter vacation. There are many all-inclusive resorts to stay where you can sit back and enjoy all the amenities such as luxurious meals and fruity cocktails without all the worry and hassle of cooking or searching for restaurants!
College Tour Trip: If you have a college bound daughter, make the most out of the time you still have left with her before she moves out and make a fun trip out of college tours. Schedule a mini vacation around her next trip to her dream school. We can help plan a fun vacation where you two can enjoy your time together before she grows up! Stay at a fun hotel, go out to a nice restaurant or order room service, visit a spa, go sightseeing, or anything else that the city has to offer.
A mother-daughter vacation is a great way to reconnect and strengthen that loving bond between the two of you. It is also a great way for you both to escape from everyday life and the rest of your family to enjoy some quality time together. No matter what you both enjoy, we can plan a great vacation just for you two! Call AurTravel today to start planning your next trip at (402) 891-6909.
Debbie Lee. Certified Travel Consultant (CTC). Debbie has been in the travel industry for more than 25 years and has held various positions. Her responsibilities include coordinating business travel, promoting AurTravel on social media, and handling general office duties. Outer Banks, NC and Disney World are favorite and frequent vacations for her and her family. Some of Debbie's travel highlights come from living in Ankara, Turkey for two years and finding exciting places to travel each weekend.


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We provide a detailed protocol for induction of long-term potentiation in the CA1 region of the hippocampus and the subsequent isolation of nuclear enriched fractions from the tetanized area of the slice. This approach can be used to determine activity dependent nuclear protein import in cellular models of learning and memory.
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Yuanxiang, P., Bera, S., Karpova, A., Kreutz, M. R., Mikhaylova, M. Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins. J. Vis. Exp. (90), e51310, doi:10.3791/51310 (2014).
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Studying activity dependent protein expression, subcellular translocation, or phosphorylation is essential to understand the underlying cellular mechanisms of synaptic plasticity. Long-term potentiation (LTP) and long-term depression (LTD) induced in acute hippocampal slices are widely accepted as cellular models of learning and memory. There are numerous studies that use live cell imaging or immunohistochemistry approaches to visualize activity dependent protein dynamics. However these methods rely on the suitability of antibodies for immunocytochemistry or overexpression of fluorescence-tagged proteins in single neurons. Immunoblotting of proteins is an alternative method providing independent confirmation of the findings. The first limiting factor in preparation of subcellular fractions from individual tetanized hippocampal slices is the low amount of material. Second, the handling procedure is crucial because even very short and minor manipulations of living slices might induce activation of certain signaling cascades. Here we describe an optimized workflow in order to obtain sufficient quantity of nuclear enriched fraction of sufficient purity from the CA1 region of acute hippocampal slices from rat brain. As a representative example we show that the ERK1/2 phosphorylated form of the synapto-nuclear protein messenger Jacob actively translocates to the nucleus upon induction of LTP and can be detected in a nuclear enriched fraction from CA1 neurons.
Synaptic N-methyl-D-aspartate-receptors (NMDARs) play a crucial role in synaptic plasticity and cell survival signaling whereas activation of extrasynaptic NMDARs can trigger neurodegeneration and cell death. These changes depend on tightly controlled/regulated activity dependent gene expression and thus require constant communication between activated synapses or dendrites and the nucleus7. The MAP kinases ERK1/2 are downstream effectors of synaptic NMDARs signaling and are involved in NMDAR-activation-induced gene expression, whereas signaling via extrasynaptic NMDAR has no or an inhibitory effect on ERK1/2 activity8,11.
There are number of proteins that have been shown to shuttle between distal dendrites and the nucleus. Many of these proteins contain a nuclear localization signal and are actively transported along microtubuli in a dynein and importin-dependent manner to the nucleus6,9. Interestingly, some of these messengers only transit to the nucleus in response to specific synaptic stimuli. For example, retrograde transport of cyclic AMP response element binding protein 2 (CREB2) is induced by chemical LTD but not LTP12. Localized NMDAR-dependent synaptic stimulation drives CREB-regulated transcriptional coactivator (CRTC1) into the nucleus, a translocation process, which is involved in long-term hippocampal plasticity4. It was recently shown that the protein messenger Jacob translocates to the nucleus after both, synaptic and extrasynaptic NMDAR activation and regulates CREB dependent gene transcription5. The synaptic or extrasynaptic origin of the signal is encoded in a posttranslational modification of Jacob. Synaptic activity induces ERK1/2 dependent phosphorylation of Jacob at a crucial serine at position 180 (pJacobS 180) which is a requisite for the subsequent translocation to the nucleus in primary hippocampal culture. Moreover, in CA1 neurons of acute hippocampal slices pJacobS 180 translocates to the nucleus after Schaffer collateral LTP but not LTD1,10. pS180 Jacob leads to an increased expression of plasticity related genes and this gene expression feeds back to synaptic function. In sharp contrast, Jacob that translocates to the nucleus after extrasynaptic NMDARs activation is not phosphorylated at Ser180 and might be associated with different protein complex in the nucleus causing 'CREB shut off' and a retraction of synaptic contacts10.
Most published studies on the nuclear import of synapto-nuclear protein messenger have been done in dissociated neuronal primary cultures. Therefore it would be interesting to see if such findings can be reproduced in physiologically more relevant conditions using hippocampal slices where neuronal connectivity and function are much better preserved. Here we present an optimized protocol for assessing LTP-dependent nuclear translocation of protein messengers by immunoblotting. This method is also suitable for analyzing activity dependent phosphorylation of proteins in a crude nuclear fraction. Specifically, the current protocol involves preparation of acute CA1 hippocampal slices, induction, and recording of LTP. Next, CA1 region is microscopically dissected to isolate the stimulated region. We combined and modified the protocol for nuclear isolation provided by CellLytic NuCLEAR Extraction Kit with changes introduced by Zhao and colleagues17. The optimized procedure includes the lysis of dissected CA1 regions in hypotonic buffer allowing cell swelling and release of nuclei. Cell lysis and nuclei morphology can be determined by microscopic examination. Nuclear enrichment is achieved by a short centrifugation step. Immunoblotting analysis with antibodies against NeuN and NSE2, specific markers of nuclear or cytosolic fractions, indicates that this approach can be used as a fast and reproducible protocol to isolate these subcellular fractions and to study very labile posttranslational modifications like protein phosphorylation. Additionally, this method is advantageous for small tissue samples deriving from dissected CA1 regions of hippocampal slices and can be used in combination to immunohistochemistry of hippocampal slices.
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1. Preparation of Acute Hippocampal Slices from Adult Rat Brain
- Anesthetize rats with isoflurane. CAUTION: Perform the procedure using a closed exicator, do not inhale isoflurane. Make sure that the animal is completely anesthetized.
- Decapitate the rat, quickly isolate the brain, and immerse it in precarbonated (95% O2 / 5% CO2 gas mixture) ice-cold Gey's solution (composition in mM: 130 NaCl, 4.9 KCl, 1.5 CaCl2·2H2O, 0.3 MgSO4·2H2O, 11 MgCl2·6H2O, 0.23 KH2PO4, 0.8 Na2HPO4·7H2O, 5 Glucose·H2O, 25 HEPES, 22 NaHCO3, pH 7.32) for 30 min1,2,10,16.
- Remove the cerebellum and part of the entorhinal cortex. Separate the cortical hemispheres with a mid-sagittal cut, then place each hemisphere down on its medial surface. Thereafter make a 50-70° cut (50-70° transverse) along the dorsal edge of each hemisphere13,14.
- Glue each hemisphere with the freshly cut surface on the slicing platform of the sectioning system. The platform should be covered by precarbogenated ice-cold Gey's solution.
- Cut 350 μm 50-70° transverse slices from anterior to posterior side using a vibratome adjusted to minimize z-axis oscillation. The hippocampal formation, subicular and entorhinal cortices, as well as the cortices that are located dorsolateral to the hippocampus will be part of the slices used for experiments13,14.
- Transfer hippocampal slices to a U-shape and submerged type incubator and incubate for at least 2 hr at 32 °C with carbogenated artificial cerebrospinal fluid (ACSF containing in mM: 110 NaCl, 2.5 KCl, 2.5 CaCl2·2H2O, 1.5 MgSO4·2H2O, 1.24 KH2PO4, 10 Glucose·H2O, 27.4 NaHCO3, pH 7.3)1,2,10,16.
2. Positioning of Electrodes, Baseline Recording, and Induction of LTP
- Transfer the hippocampal slice to a submerged type recording chamber mounted under a microscope. Perfuse (6-7 ml/min) with carbogenated ACSF for at least 30 min at 32 °C.
- Prepare glass capillary microelectrodes filled with ACSF (tip resistance is 3-5 MΩ).
- Place a glass microelectrodes filled with ACSF in the CA1 Schaffer-collateral fibers for stimulation and in the CA1 stratum radiatum for fEPSP recording1,2,10 (Figure 2). The distance between the electrodes should be about 300 μm.
- Evoke field Excitatory Postsynaptic Potentials (fEPSPs) by stimulation of Schaffer-collateral fibers with biphasic rectangular current pulses (200 msec/polarity) in a range of 3-4 V.
- Perform the maximum stimulation test by measuring the input-output relationship and define the stimulation strength as 40% of maximum fEPSP-slope values obtained and keep it constant throughout the experiment.
- Begin the baseline recording for at least 15 min after the maximum stimulation test. Measure the responses to test stimuli every minute throughout the experiment. Perform baseline recordings with low-frequency stimulation.
- Record the baseline for at least 30 min.
- For Late-LTP induction apply high-frequency 100 Hz tetanization consisting of three 1 sec stimulus trains at 100 Hz with a 5 min intertrain interval. To increase the field of stimulation within CA1 region 5 μM bicuculline can be washed in 2 min before tetanization and should be washed out immediately after last tetanization.
3. Collection of Slices after Induction of LTP and Snap Freezing
- Stop LTP recording 2 min or 30 min after three trains of tetanic stimulation inducing Late-LTP.
- Remove the electrodes and quickly transfer the slice onto a precold metal platform placed on dry ice. Repeat the procedure for a control slice kept in U-shaped incubator. CAUTION: Use gloves while working with dry ice.
- Collect each slice in a 1.5 ml tube and store at -80 °C.
4. Isolation of Nuclear Enriched Fractions from the CA1 Region of the Hippocampus
- Take 1.5 ml Eppendorf tubes with the frozen slices from -80 °C and keep them on ice.
- Add 0.5 ml of fresh cold TBS buffer containing protease (PI) and phosphatase (PS) inhibitors, into the tube. Incubate for 2-3 min and transfer to a stereomicroscope. CAUTION: Use protective gloves and a lab coat while working with PI and PS.
- Dissect the CA1 stratum pyramidale region of the hippocampus using two needles. Use one needle for holding the slice under the stereomicroscope and the second needle for cutting the CA1 area.
- Collect the dissected CA1 regions from 5 slices (for each group) into a new 1.5 ml tube containing 50 μl lysis buffer (1x hypotonic lysis buffer containing in mM: 10 HEPES, 1.5 MgCl2, 10 KCl, pH 7.9, with PI and PS). Note that this amount of material will be sufficient to run 2-3 immunoblots.
- Homogenize collected tissue by careful pipetting up and down. Use a 200 μl pipette. Incubate the lysate on ice for 5-7 min to allow the cells to swell.
- Take 2 μl of the lysate, drop on a microscopic slide, and visualize the swelling of the cells under a bright field microscope. With proper swelling of the cells the nuclei appear as round intact structures.
- Collect 20 μl of sample into a fresh 1.5 ml tube as 'homogenate fraction'. Add 8 μl of denaturing 4x SDS sample buffer.
- Centrifuge the remaining lysates at 11,000 rpm for 1 min.
- Carefully collect the supernatant from the top ('cytosolic fraction'), transfer into a new tube and add 20 μl of 4x sample buffer.
- Resuspend the pellet in 60 μl of hypotonic buffer and add 20 μl of 4x sample buffer. This fraction is referred to as a 'nuclear enriched fraction'.
- Homogenate, cytosolic, and nuclear enriched fractions can be stored at -20 °C or -80 °C for later immunoblotting.
5. Semiquantitative Immunoblotting for Synapto-nuclear Proteins
- Defreeze the samples and boil them for 5 min at 95 °C.
- Take 5 µl from each fraction and determine the protein concentration by amido black test or BCA test.
- Load equal amount of protein samples from homogenate, cytoplasmic and nuclear enriched fractions on SDS-PAGE. Samples from control and LTP slices should be placed on the same gel for direct comparison.
- Perform a standard western-blotting procedure (wet transfer is recommended).
- Probe the membranes with the antibody of choice. Subsequently the same blots (or same samples run in parallel) can be probed with a cytoplasmic marker - neuron specific enolase2 (NSE2) and nuclear marker - NeuN and a -actin antibody as a loading control.
6. Data Analysis
- Efficiency of LTP induction can be analyzed by Clampfit software. The average slope of baseline recordings was compared with the slopes after tetanization using two-way ANOVA, p <0.05 was considered significantly different. The fEPSP slope values were depicted in diagrams as the mean ± S.E.M.
- For quantification of immunoblots, scan either the autoradiographic film and analyze the integrated density of protein bands by ImageJ or fluorescence bands with a Licor system. Values of immunoreactive bands should be normalized for the loading and blotting control. For comparison of the control and LTP groups a nonparametrical Mann-Whitney U-Test might be used.
|Name of the buffer
|Concentration (mM)
|Gey's solution (pH: 7.3~7.4)
|7.6 g
|sterile filtration
|1,000 ml
|0.37 g
|0.22 g
|0.0739 g
|2.24 g
|0.0313 g
|0.2145 g
|0.9909 g
|5.96 g
|1.85 g
|ACSF (pH: 7.3~7.4)
|6.428 g
|sterile filtration
|1,000 ml
|0.1865 g
|0.368 g
|0.370 g
|0.169 g
|1.9817 g
|2.3 g
|1x Hypotonic buffer (pH: 7.9)
|0.23 g
|100 ml
|0.0304 g
|0.07455 g
Table 1. Buffers.
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Representative Results
We have previously shown that the synapto-nuclear protein messenger Jacob accumulates in the nucleus following the induction of LTP but not LTD1. Moreover, translocation of Jacob after synaptic stimulation requires activation of MAPK ERK1/2 and phosphorylation of Jacob at Ser180 (Figure 1). Phosphorylated Jacob translocates to the nucleus in an importin-dependent manner and the phosphorylated state can be preserved over extended periods of time by association with the intermediate filament αinternexin15 (Figure 1). The phospho-state of Jacob is important for the expression of activity-dependent genes and cell survival. Interestingly, activation of extrasynaptic NMDARs also drives Jacob into the nucleus but in this case Jacob is not phosphorylated at Ser180 and its nuclear accumulation induces CREB 'shut off'5,10. The results described above were obtained by protocols established in our recent study (see Karpova et al.10 for detailed description of the model).
To prove that Jacob translocates into the nucleus after induction of LTP, we induced and measured the induction of Schaffer collateral fEPSP potentiation in acute hippocampal slices and subsequently isolated neuronal nuclei from CA1 neurons (Figures 2 and 3). We compared two different time points after application of high-frequency stimulation (2 min and 30 min) and recorded the induction of LTP for each slice that was subsequently processed for nuclear isolation and western blotting (Figures 2 and 3). Enrichment of the neuronal nuclear marker NeuN can be seen in the nuclear enriched fraction prepared from the dissected CA1 region, whereas cytosolic marker neuron specific enolase2 (NSE) is mostly present in the supernatant fraction obtained after centrifugation of lysed cells (Figure 4A). The specificity of pS180 Jacob antibody has been characterized previously (for details see Karpova et al.10). pS180 Jacob levels remained unaltered in total CA1 protein homogenates and in the nuclear enriched fraction 2 min after tetanization (Figure 4B), but we found a significant increase in pJacobS 180 immunoreactivity 30 min after the induction of LTP (Figure 4C), confirming that Jacob translocating to the nucleus in this cellular plasticity model is phosphorylated at Ser180.
Figure 1. Jacob phosphorylated at S180 encodes the synaptic origin of NMDARs signals. Tetanic stimulation of hippocampal Schaffer collateral fibers results in activation of synaptic NMDARs and subsequent activation of MAPK ERK1/2 signaling cascade. Activated ERK1/2 binds to and phosphorylates Jacob at serine 180 and Jacob-ERK1/2 complex translocates to the nucleus and this correlates with enhanced CREB activity and activation of plasticity related gene expression.
Figure 2. Equipment and tools used for LTP induction and dissecting the CA1 region from hippocampal slices. A) Vibratome used for preparation of acute hippocampal slices (1 - Vibratome) B1-2) Electrophysiology and imaging setup (2 - Microscope; 3 - Micromanipulator and perfusion system; 4 - Recoding electrode; 5 - Stimulation electrode; 6 - Water objective lens; 7 - Slice holder with hippocampal slice) C) Equipment used for dissection of CA1 region from hippocampal slices (8. Stereomicroscope; 9 - Insulin syringe with needles; 10 - Small surgical scissors; 11 - Scalpel; 12 - Plastic Pasteur pipette; 13 - Thin spatula; 14-100 mm plastic culture dish filled with ice-water, and 40 mm plastic culture dish used for dissection of slices.
Figure 3. Cartoon demonstrating the experimental procedure. Numbers indicate steps in the protocol. 2.1-2.8) An acute hippocampal slice in the submerged chamber with glass electrodes placed in the stratum radiatum for potentiation of Schaffer collateral-CA1 Synapses. The inset represents the sample fEPSP analog traces, the horizontal bar indicates 5 msec and the vertical bar indicates 0.5 mV. 3.3) The slices were collected into 1.5 ml tubes after LTP recording and snap frozen. 4.3) Dissection of the CA1 region from adult rat hippocampal slices. 4.4-4.5) CA1 regions homogenized in hypotonic lysis buffer, which causes osmotic swelling of cells and release of nuclei. 4.8) Centrifugation of lysates at 11,000 rpm for 1 min. 4.10) Collection of the cytoplasmic and nuclear enriched fractions for immunoblotting. 5.3) Loading of cytoplasmic and nuclear enriched fractions on SDS-PAGE and subsequent standard western-blotting.
Figure 4. Induction of CA1 LTP leads to the accumulation of pJacS180 in the nucleus. A) Immunoblot for homogenate, cytosolic and nuclear enriched fractions of CA1 lysate probed with cytosolic and nuclear markers. B) Immunoblot analysis of pJac-S180 level in homogenate 2 min and 30 min after induction of tetanization. C) Immunoblot analysis of pJacS180 level in nuclear enriched fraction 2 min and min after tetanization. These results are part of quantification graph published in Karpova et al10. These representative immunoblots are not included in to the original publication.
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The steps described in the protocol above provide guidance how to prepare hippocampal acute sliced from young or adult rats, induce and record LTP, rapidly dissect stimulated area of slice, and prepare nuclear enriched fraction for studying activity dependent protein dynamics. This approach derives from combination of several different methods used independently from each other. We optimized a workflow and provide sufficient detail for the beginner to set up their own experiments to study the subcellular redistribution of proteins upon induction of LTP. As an example we demonstrate that the late form of LTP induces the nuclear accumulation of S180 phosphorylated Jacob. To distinguish between the phosphorylation of a pre-existing nuclear pool of a given protein and the translocation of its phosphorylated form after synaptic activity, this approach can be combined with control for total level of protein of interest in the nuclear enriched fraction. Moreover, testing samples over different time points after LTP induction might be very insightful for studying time course of protein activation/inactivation or nuclear turnover.
During the preparation of nuclear enriched fractions from stimulated slices there are several methodological issues that need to be addressed. First, to increase the number of stimulated neurons in the CA1 region and the amount of material for immunoblotting, we apply 5 µM of GABAA receptor blocker biccuculine during tetanization. Biccuculine has been shown to enhance excitatory postsynaptic potentials3.
Second, preservation of slices after induction of LTP by rapid freezing is a critical step for successful experiments because mechanical handling of slices might induce different types of activities directly correlating with changes in protein's modifications. To shorten the procedure as much as possible, we use a metal bar placed on dry ice. Slices can be transferred in a drop of ACSF using plastic a Pasteur pipette and be frozen within a very few second when placed on prechilled metal.
The third critical step is the enrichment of nuclei in CA1 lysates. We recommend to monitor cell swelling under the microscope and to find the most optimal time point. Sometimes when homogenization of tissue was not done properly, cell debris still remains in the samples. Then the nuclear pellet can be resuspended in lysis buffer again, washed for a few minutes, and then centrifuged to get a purer nuclear fraction.
Overall this protocol can help to further explorer the role of different synapto-nuclear messenger proteins in synaptic plasticity. The same procedure can be applied not only to a high frequency stimulation induced LTP but all other forms of synaptic plasticity like LTD, theta-burst LTP, short term plasticity models, and others.
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The authors have nothing to disclose.
This work was funded by the DFG (SFB 779 TPB8, Kr1879/3-1 MRK), DIP grant (MRK), EU FP7 MC-ITN NPlast (MRK), Center for Behavioral Brain Sciences (CBBS, Sahsen-Anhalt), (AK and SB), MM is a recipient of European Molecular Biology Organization (EMBO) Long-Term Fellowship (EMBO ALTF 884-2011) and Marie-Curie IEF.
|Catalog Number
|CED 1401 AD/DA converter
|Cambridge Electronics Design, UK
|Leica S4E, Germany
|Leica VT1000S, Germany
|Isolated pulse stimulator
|A-M Systems, USA
|Model 2100
|Thermo Scientific
|SDS-PAGE system
|Bright field Microscope
|Nikon Eclipse TS100
|Cell culture incubator
|Thermo Electron Corporation
|Luigs & Neumann, SM-5, Germany
|Blotting chamber and electric power supplier
|Hoefer Scientific Instruments, San Francisco, CA
|Submerged type recording chamber
|custom made
|U-shape and submerged type incubator
|custom made
|Small surgical scissors
|Thin spatula
|Plastic Pasteur pipette
|Plastic culture dish
|Bunsen beaker
|≥99.5%, p.a., ACS, ISO
|≥99.5%, p.a., ACS, ISO
|pro analysis
|pro analysis
|for molecular biology
|for molecular biology
|extra pure
|for molecular biology
|PUFFERAN, ≥99.5%, p.a.
|pro analysis
|Protease inhibitor cocktail
|Tocris Bioscience
|Primary antibodies
|Catalog Number
|rabbit (diluted 1:100)
|mouse (diluted 1:1,000)
|Cell Signaling
|rabbit (diluted 1:1,000)
|mouse (diluted 1:5,000)
|Secondary antibodies
|Catalog Number
|IgG HRP conjugated
|goat anti-mouse (1:5,000)
|IgG HRP conjugated
|goat anti-rabbit (1:5,000)
- Behnisch, T., et al. Nuclear translocation of Jacob in hippocampal neurons after stimuli inducing long-term potentiation but not long-term depression. PLoS One. 6, (2), e17276 (2011).
- Cai, F., Frey, J. U., Sanna, P. P., Behnisch, T. Protein degradation by the proteasome is required for synaptic tagging and the heterosynaptic stabilization of hippocampal late-phase long-term potentiation. Neuroscience. 169, (4), 1520-1526 (2010).
- Chapman, C. A., Perez, Y., Lacaille, J. C. Effects of GABA(A) inhibition on the expression of long-term potentiation in CA1 pyramidal cells are dependent on tetanization parameters. Hippocampus. 8, (3), 289-298 (1998).
- Ch'ng, T. H., Uzgil, B., Lin, P., Avliyakulov, N. K., O'Dell, T. J., Martin, K. C. Activity-dependent transport of the transcriptional coactivator CRTC1 from synapse to nucleus. Cell. 150, (1), 207-221 (2012).
- Dieterich, D. C., et al. Caldendrin-Jacob: a protein liaison that couples NMDA receptor signalling to the nucleus. PLoS Biol. 6, (2), e34 (2008).
- Fainzilber, M., Budnik, V., Segal, R. A., Kreutz, M. R. From synapse to nucleus and back again--communication over distance within neurons. J. Neurosci. 31, (45), 16045-16048 (2011).
- Hardingham, G. E., Bading, H. Synaptic versus extrasynaptic NMDA receptor signalling: implications for neurodegenerative disorders. Nat. Rev. Neurosci. 11, (10), 682-696 (2010).
- Ivanov, A., et al. Opposing role of synaptic and extrasynaptic NMDA receptors in regulation of the extracellular signal-regulated kinases (ERK) activity in cultured rat hippocampal neurons. J. Physiol. 57, 789-798 (2006).
- Jordan, B. A., Kreutz, M. R. Nucleocytoplasmic protein shuttling: the direct route in synapse-to-nucleus signaling. Trends Neurosci. 32, (7), 392-401 (2009).
- Karpova, A., et al. Encoding and transducing the synaptic or extrasynaptic origin of NMDA receptor signals to the nucleus. Cell. 152, (5), 1119-1133 (2013).
- Kim, M. J., Dunah, A. W., Wang, Y. T., Sheng, M. Differential roles of NR2A- and NR2B-containing NMDA receptors in Ras-ERK signaling and AMPA receptor trafficking. Neuron. 46, 745-760 (2005).
- Lai, K. O., Zhao, Y., Ch'ng, T. H., Martin, K. C. Importin-mediated retrograde transport of CREB2 from distal processes to the nucleus in neurons. Proc. Natl. Acad. Sci. U.S.A. 105, (44), 17175-17180 (2008).
- Leutgeb, J. K., Frey, J. U., Behnisch, T. LTP in cultured hippocampal-entorhinal cortex slices from young adult (P25-30) rats. J. Neurosci. Methods. 130, (1), 19-32 (2003).
- Leutgeb, J. K., Frey, J. U., Behnisch, T. Single cell analysis of activity-dependent cyclic AMP-responsive element-binding protein phosphorylation during long-lasting long-term potentiation in area CA1 of mature rat hippocampal-organotypic cultures. Neuroscience. 131, (3), 601-610 (2005).
- Perlson, E., Hanz, S., Ben-Yaakov, K., Segal-Ruder, Y., Seger, R., Fainzilber, M. Vimentin-dependent spatial translocation of an activated MAP kinase in injured nerve. Neuron. 45, (5), 715-726 (2005).
- Xiang, Z., et al. Long-term maintenance of mature hippocampal slices in vitro. J. Neurosci. Methods. 98, (2), 145-154 (2000).
- Zhao, M., Adams, J. P., Dudek, S. M. Pattern-dependent role of NMDA receptors in actin potential generation: consequences on extracellular signal-regulated kinase activation. J. Neurosci. 25, (30), 7032-7039 (2005).
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New buildings, University Club on the way
Several major building projects are getting underway this fall, including a new Liberal and Creative Arts (LCA) building, the University's first new academic building in 25 years. Another project, located on Holloway and Varela avenues, is a student housing facility that when completed will house more than 560 students as well as a variety of neighborhood-serving retail and commercial services. Each of these projects is expected to take 24 months and will be completed by fall 2020.
In addition, a smaller-scale project will revive an old campus tradition. The University Club, a lounge for staff and faculty members, has been absent from campus for more than a decade due to space issues. A portion of the Cesar Chavez Student Center that was used by the bookstore is being converted into a new and improved University Club. It will open in the latter part of the fall semester or early in spring 2019.
Go here to read an in-depth article about the new building projects and what they'll add to campus.
Grad student receives CSU honor
Geography & Environment graduate student Salma Abdel-Raheem has been given the $6,000 Trustees' Award for Outstanding Achievement, the CSU's highest recognition of student achievement. The awards provide donor-funded scholarships to students who demonstrate exemplary academic performance, personal accomplishments, community service and financial need. Abdel-Raheem and 22 other awardees — one from each CSU campus — were honored last week at a CSU Board of Trustees meeting in Long Beach, California.
At a young age, Abdel-Raheem emigrated from Egypt to the U.S. with her family. When she arrived at SF State to earn her master's degree in geographic information science, she found a surprising way to fulfill that mission: maps. For her master's thesis, she's creating digital maps for a diverse community on St. George's Caye, a small island off the coast of Belize. Abdel-Raheem visits the island to organize meetings with residents, mapping out their shared understanding about their surroundings: everything from traditional boundaries to development projects to protected mangrove trees. Those maps can then form the basis for decisions about the future of the community. Abdel-Raheem has also been working with the Anthropocene Institute, an environmental NGO, to map boat traffic around protected areas off the coast of California in order to help park regulators and state officials better manage the waters.
"Salma always surprises me with her depth of knowledge and commitment to her research," said her advisor, Professor of Geography & Environment Ellen Hines. "She is extremely self-assured.
New director of Health Promotion & Wellness named
The Division of Student Affairs (DSA) is proud to welcome Karen Boyce to SF State as the new director of Health Promotion & Wellness (HPW). A graduate of Columbia University School of Social Work, Boyce comes to campus after more than seven years with the New York City Department of Health and Mental Hygiene, where her most recent position was deputy director of evidence based programs within the Center for Health Equity. In this work, Boyce oversaw community wide sexual health program implementation and training reaching over 130 partners and targeting 13,000 young people. She has extensive experience coordinating large-scale projects to improve community health, as well as supervising staff. Her first day at SF State was July 23.
DSA would also like to extend special thanks to Rick Nizzardini, who served as interim director, for his excellent leadership in the overall creation of HPW. Over the past three years, Nizzardini adroitly brought together staff from two distinctive departmental cultures and developed shared vision, mission and values that enhanced how the team approaches their work to improve student health and wellbeing. On July 1, Nizzardini returned to Counseling & Psychological Services, where he resumed his role as residential life counselor.
Academic Master Plan (AMP) seeks your participation
Senate Chair Nancy Gerber and Provost Jennifer Summit, co-chairs of the academic master planning process, invite your participation in articulating a vision that will drive the University's academic identity into the future. The Academic Master Plan Steering Committee is now accepting nominations for working group members. The time commitment will be roughly one to two hours a week, with the exception of breaks and holidays, from October 2018 to March 2019. If you would like to nominate yourself or a colleague, please complete the online form. The Steering Committee will begin assembling the working groups on Wednesday, Sept. 19. Learn more about the process on the AMP website.
New ergonomic self-assessment software
Working at an improperly aligned workstation could cause you to develop a variety of muscle strains and stress-related injuries. So it's important to identify issues with your workspace before problems arise. On Oct. 1, Enterprise Risk Management (ERM) will begin implementation of a new ergonomic self-assessment software tool, Work Safety Plus. Employees will be asked to take a 30-minute, online self-assessment of their workstations. The self-assessment is designed to assist with adjustments to your workstation to make it more ergonomically-friendly.
Assessment results will be reviewed by ERM's Ergonomics team and in-person. Follow-up assessment will be provided to those users who are still experiencing workstation discomfort. Recommendations for additional workstation adjustments and improvements will be discussed.
To access the self-assessment tool, please request a link by emailing email@example.com or by visiting the ERM Ergonomics homepage.
Become a Safe Zone ally
The SF State Safe Zone Ally program is looking for staff, faculty and administrators to become safe zone allies for the campus LGBTQQIA (Lesbian, Gay, Bisexual, Transgender, Queer, Questioning, Intersex and Ally) community. The Safe Zone Ally program's mission is to foster a safe campus environment by building a support network for people of all gender and sexual identities. Safe Zone allies are active and visible volunteers who are open to talking to members of the LGBTQQIA community in a safe and supportive environment. To become a Safe Zone ally, volunteers must attend one of the training sessions that are offered throughout the year. The next training will be on Friday, Nov. 9, from 9 a.m. to 3 p.m. A light lunch will be provided. For further details about the training or to register for the training, contact Rick Nizzardini at email@example.com or ext. 5-4415. (You must pre-register in order to participate). You can also get more information about the Safe Zone Ally program by checking out its website at safezone.sfsu.edu.
National Preparedness Month
Environmental Health and Occupational Safety wants to remind the campus community that September is National Preparedness Month. Plan ahead, know the hazards and keep safe. The Occupational Safety and Health Administration offers a number of emergency preparedness guides for work and home. Learn more online.
Academic Senate meeting, Sept. 18
The Academic Senate will meet Tuesday from 2 to 3:30 p.m. in the Nob Hill Room of the Seven Hills Conference Center. An open-floor period from 2 to 2:10 p.m. will provide an informal opportunity to raise questions or make comments to Senate officers or University administrators. Please arrive promptly at 2 p.m. A reception for new Senators at Seven Hills will follow immediately after adjournment of the plenary. The agenda will include:
- Senate chair and standing committee reports
- Jennifer Shea, associate professor of public administration, on the Political Engagement & Learning Assessment Project (2:30 p.m.)
- A visit from Wendy Tobias, director of the Disability Programs & Resource Center (2:45 p.m.)
- A visit from President Leslie Wong (3 p.m.)
- A report from the Academic Senate of the California State University System from Professor of Gerontology Darlene Yee-Melichar, American Indian Studies Associate Professor Robert Keith Collins and Professor of Mechanical Engineering Dipendra Sinha
The complete agenda and support documents for the meeting are available online.
Prescription Drug Take Back Day, Sept. 19
Do you know how to safely use, store and dispose of prescription drugs? Stop by the first annual Prescription Drug Take Back Day at Malcolm X Plaza from noon to 2 p.m. on Wednesday, Sept. 19, to learn more from Health Promotion and Wellness, Counseling and Psychological Services and the Student Health Center. The first 30 people who "take back" their leftover or expired prescription drugs to the safe disposal kiosk in Student Health Services will receive a free pill-lock container. They will also be entered in a drawing to win a lock box that can help store prescription drugs safely.
Banned Books Week, Sept. 23-29
The J. Paul Leonard Library invites the campus community to explore banned books and celebrate the freedom to read during Banned Books Week, Sept. 23-29. Visit the display of books for young readers that have been banned or challenged (located on the fourth floor's east wall display cases in the Willie L. Brown Jr. Hall) then come down to the first floor and take a "mugshot" selfie for getting caught reading banned books. Don't forget to post your mugshot to social media with the hashtag #JPLLBanned.
Bachelor of Arts in Social Work information session, Sept. 27
The School of Social Work (SSW) will be holding an information session for its Bachelor of Arts in Social Work (BASW) program on Thursday, Sept. 27, from 6:15 to 7:30 p.m. in LIB 121. Anyone interested in pursuing a social work degree is welcome to attend. Off-campus participants may also Zoom in. Please see the SSW website for log-in details.
Global Museum program on the Amazon rainforest, Oct. 2
Jeremías Petsein Peas, a leader of the Achuar indigenous people from the Peruvian Amazon rainforest, will visit campus to make a presentation on Tuesday, Oct. 2. The Achuar people are suffering from the impacts of decades of oil pollution in their rivers and food sources and are now fighting against new oil projects in their sacred territories. Come hear about the cultural, legal and political efforts to defend their territory, in turn protecting the environment for their children and all of humanity. The presentation will be last from 5:30 to 7:30 p.m. in HUM 133 and will be followed by a reception in the Global Museum (FA 203). Light refreshments will be provided.
This event is co-sponsored by Amazon Watch, a nonprofit founded in 1966 to protect the rainforest and advance the rights of indigenous peoples in the Amazon Basin. The event is free and all are welcome. Send an optional RSVP to email@example.com.
EOS Center 40th Anniversary Celebration, Oct. 4
The Estuary & Ocean Science (EOS) Center will celebrate its 40th anniversary at the Romberg Tiburon Campus with a gala from 6:30 to 9:30 p.m. Thursday, Oct. 4. There will be music, food and drinks, auctions and more. The emphasis is on fun and friend-making to support development. Tickets are $75. More information is available online.
Monday, Sept. 17
Rights and Wrongs: A Constitution and Citizenship Day Conference: the J. Paul Leonard Library and the Cesar Chavez Student Center
Tuesday, Sept. 18
Rights and Wrongs: A Constitution and Citizenship Day Conference: the J. Paul Leonard Library and the Cesar Chavez Student Center
Wednesday, Sept. 19
Lecture: Mindfulness for Musicians: 1:10 p.m. in Knuth Hall, Creative Arts building
Nonprofit Management Information System: 6 p.m. at the Downtown Campus
The Best of Czechoslovak Cinema: "…and the Fifth Horseman Is Fear": 6:30 p.m. in the Coppola Theatre, Fine Arts building
Thursday, Sept. 20
SF State Park(ing) Day: 10 a.m. to 3:30 p.m., various campus locations
Poetry reading: MK Chavez and Heather June Gibbons: 7 p.m. at the Poetry Center (HUM 512)
Friday, Sept. 21
Recital: Randall Kohl, guitar: 1:10 p.m. in Knuth Hall, Creative Arts building
Nonprofit Management Certificate Program: Volunteer Management Course: 6 p.m. at the Downtown Campus
Saturday, Sept. 22
Opening reception: "All Together Now" art exhibition: 1 p.m. at the Fine Arts Gallery, Fine Arts building
Sunday, Sept. 23
Logan talks Amazon
Labor and Employment Studies Chair John Logan was quoted in a BuzzFeed article about working conditions in Amazon warehouses. Logan commented on the growing interest by some in unionizing Amazon employees. "Unions are … coming to the conclusion that they really can't afford to ignore Amazon," Logan said. "Amazon is becoming such a significant player in so many different parts of the economy — in the grocery sector, in home delivery and commerce. I think in the future you will see more of a sustained effort, not just from unions, but with all of these other different community organizations."
Sueyoshi explores CSU funding
Professor and Interim Dean of the College of Ethnic Studies Amy Sueyoshi wrote an op-ed for Diverse Issues in Higher Education about the dangers of underfunding the California State University system. "In a city where coffee and a doughnut could cost $10 and a 500 square foot one bedroom condo frequently sells for $800,000, shouldn't there be more dollars in the state coffers for the CSU?" Sueyoshi wrote. "Moreover, analysts have also associated high gross state product, low unemployment rates, and Democratic Party control to increased higher education spending. Why then does California – a state that fulfills nearly all these requirements – implement successive cuts to the CSU, even as waves of college-ready seniors are coming out of high schools?" To read the op-ed in its entirety, click here.
Jungle Express for gene expression
Associate Professor of Biology Joseph Chen, in collaboration with researchers at the Department of Energy's Joint BioEnergy Institute, the Lawrence Berkeley National Laboratory and the Lawrence Livermore National Laboratory, developed a new gene expression system that outperforms conventional systems. Their work, published Sept. 6 in the journal Nature Communications, describes the bottom-up engineering of Jungle Express, a versatile platform that enables efficient gene regulation in diverse bacteria for basic science and biotechnological research. The collaboration was made possible partly by funding from the CSU Program for Education and Research in Biotechnology (CSUPERB) and SF State's Leave with Pay program.
New book for Salama
Professor of Modern Languages and Literatures Mohammad Salama is the author of a new book, "The Qur'an and Modern Arabic Literary Criticism: From Taha to Nasr" (Bloomsbury Publishing USA). Walid A. Saleh, professor of Islamic studies at the University of Toronto, calls it "one of the best studies to appear on the central debate in modern Islam: How should Muslims study the Qur'an?"


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In our next artist spotlight, we chat to US darkwave electronic producer, Verasect about his music and inspirations.
S] How are you today?
I'm doing well. Just got a remix back from Ofdream today that sounds great. Also, Dobie's remix was mastered and will be putting up on Soundcloud pretty soon. This is the first time I've had my songs remixed and it's exciting and humbling at the same time.
S] Talk us through your inspirations outside of music, think specific people, movies and/or places?
I'm definitely inspired just as much by film as I am by other music. I was a filmmaker when I was younger and studied film in university. Just like music I'm drawn to dark and somewhat strange films that put me in a different emotional state. When I first started writing music I was going to a lot of art house theaters and caught a showing of City of Lost Children by Jean-Pierre Jeunet. It blew me away and reflected in my dark, heavy industrial musical projects back then.
Now I would say travel inspires me the most musically. It's really the only time I get true solitude and time to think. That's when I compose melodies and write lyrics that I will later build into songs in the studio. For Sleep Stories, I did a lot of writing while traveling through Iran. It gave me the time to play back events in my life and form them into songs.
S] What about working with Dave Oglivie?
Dave's a legend and I'm honoured to work with him. He's mixed so many of albums I grew up listening to and I knew who he was even before I started making music because artists would praise him in interviews. I'm very particular and a total pain in the ass to work. We went back and forth in emails so many times and I was worried he would become frustrated. But he's super polite and understanding. I hope I can come out to Vancouver and sit in on the mix sessions for the next album.
S] What advice would you give to emerging producers coming out, and what software did you use?
Being great isn't enough anymore, be different. Software is cheap and YouTube is overflowing with tutorials on how to sound like top artists, so there is a surplus of professional sounding music. But I honestly forget it five minutes after listening. I think there will be a backlash against super polished electronic music after the arena EDM generation, and we'll start seeing some real innovation.
Software-wise, I work in Cubase and try to avoid loop-based sequencers. In any software, it's easy to fall into looping too much but Cubase is more designed for tracking traditional bands and I try to approach songwriting from a rock direction as opposed to an EDM direction.
I'm very light on soft synths and love hardware synthesizers. I try to start songs away from the computer and move it into a sequencer as late in the process as possible. The exception to this is when I travel and only have my iPad with me.
S] What challenges do you face as an artist now?
I think like most artists I struggle with balancing writing music and promotion. I read recently that you need to be put a video a day on YouTube to get noticed. Not a video of your music but you just talking to the camera. I can't think of anything I'd like to do less and it makes my heart die. I understand that promotion will always be a part of art but having it be so intrusive in your daily life is relatively new and not something I'm comfortable with.
S] Talk us through the inspirations behind the tracks, 'Aeon' and 'Catch Your Breath'?
'Catch Your Breath' was the first song I wrote as Verasect. I was looking for a new sound and to move away from aggressive industrial music and do something more emotional and dreamy. So I decided to write about my most painful recent memory, a breakup I had in New York several years back. Rather than write about the whole ordeal I decided to put myself back in that particular moment. The moment when I found out about a slew lies and deception that was completely unforgivable and I knew it was over. I remember that moment vividly and having to sit down, catch my breath and let it all sink in.
'Aeon' is based on a concept in the novel Ubik by Phillip K Dick that I found incredibly strange and haunting. In the book, when you die you are brought to a facility called a moratorium, revived and placed in a dream-like state. Your family and loved ones can come to visit and "wake" you up to talk via a headset. But the amount of time you can be awake is finite. It is used sparingly until you ultimately pass and can no longer be woken up.
I read that book when I was a kid and that part always stuck with me. While it would be great to prolong life, are you just extending the pain for both sides? 'Aeon' is about how it would be to have someone you love in that state of being between life and death.


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Voodoo love spells with honey to strengthen your love ties
It's always much easier to strengthen your love ties or relationship using my voodoo love spells with honey because its good at increasing or stimulating your passion for one another and also increasing the love and commitment for one another. For all those in relationship with lost love I restore them back using these voodoo love spells with honey. I make your relationship become as sweet as honey and you partner will never get enough of you because he or she will keep on wanting you more and more. So I suggest you don't lose your relationship if you still want it because I can still help you fix it even when therapy has failed both of you. All you need is to contact me through the contact form below and I will make it happen for you as soon as possible.
Voodoo love spells with honey to make someone love you unconditionally
Unconditional love is the love with no boundaries the love that will keep you young and flourishing throughout the ages. So if you feel bad, emotionally down because you are being treated young and unwanted then its time you cast these voodoo love spells with honey. Sometimes relationships start strong but as time goes on they lose the affection and connection clearly showing how hard it is to find true love these days because most people are just interested in sex or wealth. However both people can think the same that is why casting my voodoo spell is a remedy to put the situation in your favor.
Voodoo love spells with honey to make your n laws love you
Are you being hated by your in laws because they feel you are not worth being family to them? If you want to marry your partner and you don't care what they think of you then its time you silence them with these voodoo love spells with honey. These are very sweet and aggressive spells ready to transform anger or hate in to love. Their perception towards you is completely going to change and they are going to start recognizing you as their family. If you want them to also beg you for forgiveness for how they treated you I can still do that for you.
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