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Background During angiogenesis, the development of fresh blood vessels ships from
Background During angiogenesis, the development of fresh blood vessels ships from existing types, endothelial cells distinguish in to hint and stalk cells, after which 1 hint cell qualified prospects the develop. chemotaxis. In both these sprouting versions the endothelial cells automatically migrate forwards and backwards within seedlings, recommending that suggestion cell ruling might happen as a part impact of sprouting. In compliance with various other fresh findings, in our simulations the cells propensity to take up the suggestion placement Rotundine IC50 can end up being governed when two cell lines with different amounts of reflection are adding to sprouting (mosaic sprouting assay), where cell behavior is normally governed by a basic VEGF-Dll4-Level signaling network. A conclusion Our modeling outcomes recommend that suggestion cell ruling can occur automatically credited to the stochastic movement of cells during sprouting. Hence, suggestion cell overtaking and sprouting design may end up being interdependent and should end up being studied and interpreted in mixture. VEGF-Dll4-Level can regulate the capability of cells to take up the suggestion cell placement in our simulations. We recommend that the function of VEGF-Dll4-Level signaling might not really end up being to regulate which cell ends up at the suggestion, but to assure that the cell that arbitrarily ends up at the suggestion placement acquires the suggestion cell phenotype. Electronic ancillary materials The online edition of this content (doi:10.1186/s12918-015-0230-7) contains supplementary materials, which is obtainable to authorized users. reflection or fairly low amounts of reflection are even more most likely to end up at the suggestion placement in a Notch-dependent style, recommending that the competitive potential of cells to consider up the suggestion placement is normally controlled by the signaling systems consisting of VEGF, Notch and Dll4. VEGF affects suggestion cell selection by causing Dll4 creation upon VEGFR2 account activation [7]. Level account Rotundine IC50 activation in border cells down-regulates reflection [8]. Using this signaling network, computational modeling by Jakobsson et al. [5] recommended that suggestion cell ruling is normally governed by Level activity. In a follow-up model, Bentley et al. [9] examined the function of cell-cell adhesion and junctional reshuffling, Rotundine IC50 using a alternative of the Cellular Potts Model, enabling cells to get along one another within a preformed cylindrical empty develop. By evaluating different combos of systems, their modeling outcomes recommended a even more complete regulatory system for suggestion cell ruling: 1) VEGFR2 signaling causes endocytosis of VE-cadherin, which decreases cell-cell adhesion. 2) Level activity reduces expansion of polarized actomyosin protrusions towards the develop suggestion. Therefore, these Rotundine IC50 outcomes recommend that Dll4-Level and VEGF signaling highly regulate suggestion cell ruling. In obvious contradiction with this presentation, Arima et al. [6] discovered that suggestion cell overtake prices had been not really affected by addition of VEGF or by inhibition of Dll4-Level signaling, although additional actions of sprouting kinetics had been inspired, elizabeth.g., develop expansion price and cell speed. Arima et al. [6] shown intensive cell monitoring data of cell motion and placement during angiogenic sprouting and discovered that specific ECs migrate ahead and backwards within the develop at different velocities, leading to cell combining and ruling of the suggestion placement. Therefore, suggestion cell ruling might occur automatically from group cell behavior traveling angiogenic sprouting. To help translate these outcomes, we 1st analyzed to what degree suggestion cell ruling happens in existing computational versions, without producing any extra presumptions (Fig.?1a). Although the specific mobile systems generating angiogenesis are incompletely realized still, a range of computational versions provides been suggested each addressing an substitute, related mechanism [10 often, 11]. In lack of a defined sprouting model, we likened two prior Cellular Potts versions [12, 13]. In the Rabbit Polyclonal to CA12 initial model, the cells secrete a chemical substance sign that draws in encircling cells via chemotaxis. Servings of the membrane layer in get in touch with with nearby cells become insensitive to the chemoattractant [13]. The model forms seedlings of one or two cell diameters thickness (Fig.?2a, ?,c).c). The second model hypothesizes that non-inhibited chemotaxis suffices to form angiogenesis-like seedlings, if the cells possess an elongated form [12] (Fig.?2b, ?,dd). Fig. 1 Review of the workflow. We analyzed the natural relevance Rotundine IC50 and the traveling systems of suggestion cell ruling. a As a first stage, we asked whether suggestion cell ruling can become a part impact of sprouting. We analyzed suggestion cell ruling.
Precursor B-lineage extreme lymphoblastic leukemia (pre-B ALL) impacts hematopoietic advancement and
Precursor B-lineage extreme lymphoblastic leukemia (pre-B ALL) impacts hematopoietic advancement and therefore is associated with defense insufficiencies that may end up being further exacerbated by chemotherapy. autologous bone tissue marrow transplants. from pediatric ALL examples at analysis, relapse and remission, and possess significant antibody-dependent and non-antibody reliant cytotoxicity in an autologous establishing. Components AND Strategies Manifestation evaluation and circulation cytometry The 64657-21-2 IC50 -BAFF-R antibody utilized for ADCC assays was offered by Novartis and offers been explained. 13 To determine the percentage of NK cells in examples, cells had been cleaned, treated with individual FcR preventing reagent (Miltenyi Biotec, Bergisch Gladbach, Indonesia) for 10 a few minutes and after that tarnished with Compact disc56-PE and Compact disc3-PerCP antibodies (Biolegend, San Diego, USA). For 64657-21-2 IC50 BAFF-receptor phrase, cells had been tarnished with Compact disc19-FITC, BAFF-R-PE and Compact disc10-APC (BD Biosciences, San Jose, California). Cells had been analyzed by stream cytometry on an Accuri stream cytometer (Ann Arbor, MI, USA). We examined effector cell quantities on a FACS Canto II (BD Biosciences) using Compact disc45-PerCP, Compact disc19-APC, Compact disc10-FITC, BAFF-R-PE, Compact disc56-FITC, Compact disc16-PE, Compact disc3-APC (BD Biosciences). For TSPAN8 phrase of Compact disc3, Compact disc56, NKG2N, CD16 and NKp46, non-expanded PBMCs and corresponding extended NK cells had been cleaned, treated with individual Fc preventing reagent for 10 a few minutes and tarnished with Compact disc3-PerCP after that, Compact disc56-FITC, NKG2D-APC, NKp46-PE-Cy7, (Biolegend) and Compact disc16-BV510 (BD Bioscience, San Jose, California). Cells had been examined on a FACS Canto II stream cytometer (BD Biosciences). For evaluation of IFN and Compact disc107a, eexpanded NK cells (1 a 106) from ALL individual examples had been triggered with nothing at all, or with US7 cells (2×105) in the existence or lack of 10 g/ml individual control IgG Ab or BAFF-R mAb as indicated for 1 human resources, with addition of Compact disc107a-PE antibodies (BD Bioscience, San Jose, California). Non-expanded PBMCs had been activated with PMA (2.5 g/ml) and ionomycin (1.0 g/ml) as a positive control. Cells had been after that incubated for an extra 3 l at 37C with brefeldin A (eBioscience, San Diego, California) and monensin (Golgi-Stop, BD Biosciences). After cleaning and addition of Fc stop (BD Biosciences), cells had been discolored with Compact disc56-FITC, Compact disc16 BV510 and Compact disc3-PerCP for 30 minutes. After fixing and washing, cells had been permeabilized with a BD Cytofix/CytopermTM package, adopted by intracellular yellowing for -interferon (-IFN)-APC (BD Bioscience, San Jose, California) for an extra 30 minutes. Examples had been examined on a FACS Canto II circulation cytometer (BD Biosciences). Cell tradition US7 cells possess been previously explained. 14 ALL individual examples had been acquired on Children's Medical center Los Angeles IRB-approved protocols. Ficoll-Paque separated peripheral bloodstream mononuclear cells (PBMCs) or bone tissue marrow mononuclear cells (BMMCs) had been examined newly or kept in water nitrogen. OP9 mouse stromal cells (CRL-2749) had been from the American Type Tradition Collection (Manassas, Veterans administration). PBMCs or BMMCs from ALL individuals had been straight cultured with irradiated OP9 cells. Cell development became obvious after a adjustable lag period of up to 2 weeks. Co-culture of human being ALL cells with OP9 cells was in MEM- moderate supplemented with 20% FBS, 1% L-glutamine and 1% penicillin/streptomycin (Existence Systems, Grand Isle, Ny og brugervenlig). We utilized plenty of FBS that we experienced examined for capability to maintain ideal development of previously defined patient-derived pre-B ALL cells 14 for co-culture 64657-21-2 IC50 64657-21-2 IC50 with principal individual ALL cells. NK cells were expanded as described previously. 8, 9 Quickly, we began with 2×106 to 2×107 mononuclear, Ficoll-purified cells (PBMCs or BMMCs) for co-culture with irradiated T562 clone 9.mbIL-21 cells, as artificial antigen-presenting cells (aAPC). Co-cultures had been harvested in RPMI-1640 moderate supplemented with 10% FBS, 64657-21-2 IC50 1% L-glutamine, 1% penicillin/streptomycin (Lifestyle Technology, Grand Isle, Ny og brugervenlig) and 50 ng/ml recombinant individual IL-2 (PeproTech, Rocky Mountain, Nj-new jersey). Irradiated T562 clone 9 Freshly. mbIL-21 cells were added every single complete week. Recombinant individual IL-15 was bought from PeproTech (Rocky Mountain, Nj-new jersey). Concentrations of secreted IFN from NK.
Background Growth heterogeneity and the plasticity of cancers cells present issues
Background Growth heterogeneity and the plasticity of cancers cells present issues for effective clinical therapy and medical diagnosis. and Cdk2. Dual inhibition of Cdk1 and Cdk2 using medicinal inhibitor or RNAi led to modulation of the cell routine and advertising of NED. We confirmed that the cyclic adenosine 3 further, 5-monophosphate (cAMP)-mediated path is definitely triggered in the high-density circumstances. Significantly, inhibition of cAMP signaling using a particular inhibitor of adenylate cyclase, MDL-12330A, removed the advertising of NED by high cell denseness. Conclusions together Taken, our outcomes indicate a fresh romantic relationship between cell routine attenuation and advertising of NED and recommend high cell denseness as a result in for cAMP signaling that can mediate AT7519 reversible NED in prostate malignancy cells. by numerous stimuli, such as androgen exhaustion [6,7], improved amounts of interleukin-6 (IL-6) [8], service of Wnt [9] and EGF [10] signaling paths, service of the cyclic adenosine 3, 5-monophosphate (cAMP) signaling path [11-13], or ionizing rays [14,15]. In addition, many genetics and transcription elements had been demonstrated to become included in NED, for example protocadherin-PC and the transcription elements Foxa2 and NeuroD1 (described in [2]). Androgen exhaustion, which AT7519 induce NED, is definitely connected with cell routine police arrest in G1 stage [16,17]. This cell routine police arrest is definitely connected to modulation of well-known cell routine government bodies included in G1 stage development and the G1 to H stage changeover [16,18]. Another system that contributes to cell routine police arrest is ARHGAP1 definitely the trend of get in touch with inhibition. High-density farming is definitely connected with police arrest in G1 stage that is definitely followed by reduced Cdk2 and Cdk4 activity, actually in malignancy cells that are refractory to the standard get in touch with inhibition displayed by regular cells. Furthermore, cell thickness can impact intracellular signaling, as proven by density-dependent adjustments in intra- and extra-cellular distribution of cAMP [19]. In the present research, we concentrated on the function of cell routine modulation in the regulations of NED in prostate cancers cells. We demonstrated that androgen cell and exhaustion routine modulation mediated by high cell thickness both marketed NED, which was confirmed by elevated reflection of quality indicators both in AR-positive and AR-negative prostate epithelial cell lines of different beginning. We discovered an essential function of Cdk2 and Cdk1 activity in promoting NED by cell cycle attenuation. Finally, our outcomes recommend a function of cAMP AT7519 signaling account activation in NED advertising by high cell thickness in AR-positive prostate cancers cell lines. Used jointly, our data determine a book condition leading to the advertising of NED in prostate malignancy cells and define particular molecular systems that determine this procedure. Outcomes Androgen exhaustion and high cell denseness promote NED features of prostate malignancy cells NED guns possess varied biologic features: -enolase is definitely one of the iso-enzymes of the glycolytic enzyme enolase, which catalyzes the transformation of 2-phospho-glycerate to phosphoenolpyruvate and is definitely discovered in adult neurons (described in [20]); cytoskeletal proteins tubulin -III is definitely an early gun of neuronal difference [21]; chromogranin A is definitely a prohormone indicated in endocrine cells and peptidergic neurons that mediates granule development (described in [22]); and L-dopa decarboxylase is definitely an enzyme included in the activity of dopamine, serotonin, and tryptamine that was demonstrated to interact with androgen receptor (AR) [23]. We determined to assess many different guns of NED at both the proteins and mRNA level because the appearance of different NED guns may not really correlate in every fresh set-up, as was demonstrated for -enolase and chromogranin A in LNCaP cells going through NED [24]. Androgen exhaustion in LNCaP cells elevated proteins amounts of the utilized NED indicators -enolase broadly, tubulin -III [25] (Amount?1A, correct -panel) and mRNA amounts of the NED guns -enolase (ENO2) and aromatic L-amino-acid decarboxylase (DDC) [26] (Number?1B). Remarkably, NED guns had been also up-regulated at both proteins and mRNA amounts in cells grown at high denseness in the existence of androgens (FBS, day time 8 and 16). Significantly, related results of androgen exhaustion and high denseness in advertising NED had been noticed in another prostate tumor cell range, LAPC-4 AT7519 (Number?1A, M). Immunofluorescence evaluation of tubulin -III appearance demonstrated a positive sign in LNCaP cells grown for 16?times under androgen-depleted circumstances in dextran/charcoal-stripped serum-containing moderate (16d CS) or under large cell denseness circumstances after farming for 16?times in FBS (16d FBS), where the indicators were detected.
The severe bone destruction and resorption that may occur in Osteoarthritis
The severe bone destruction and resorption that may occur in Osteoarthritis of the Temporomandibular Joint (TMJ) is associated with significant pain and limited joint mobility. morphological differences of each resorption model with the original asymptomatic control. The size of each AS-605240 simulated defect was analyzed and the values obtained compared AS-605240 to the true AS-605240 defect size. The statistical analysis revealed very high probabilities that mean shape correspondence measured defects within 0.5mm of the true defect size. 95% confidence intervals (CI) were (2.67,2.92) and (5.99,6.36) and 95% prediction intervals (PI) were (2.22,3.37) and (5.54, 6.82), for 3mm and 6mm simulated defects respectively. The next part of the scholarly study applied shape correspondence solutions to a longitudinal sample of TMJ OA patients. The mapped longitudinal levels of TMJ OA development determined morphological subtypes or variations, which may describe the heterogeneity from the scientific presentation. This study validated shape correspondence as a strategy to and predictably quantify 3D condylar resorption precisely. ? ? fell inside the interval. Predicated on the test details, the CI was attained by where = ? 1. The self-confidence interval didn't have to support the accurate mean because it assessed the possibility the fact that intervals support the accurate mean. Quite simply, if 100 examples were drawn and its own CIs calculated, around 95 CIs would support the true mean after that. 95% PI supplied an estimate of the interval a upcoming observation of the random adjustable (if any) would fall within the number of the period. It could be regarded as a self-confidence period for prediction. The prediction period is certainly always wider compared to the self-confidence interval due to the additional doubt for prediction. PI could be computed by > 0.05). Body 6 displays semitransparent overlays of the common models for still left and correct condyles, demonstrating that no relevant distinctions between observers can be found. Body 6 Semitransparent overlays of typical models for every observer (blue and crimson) present no significant distinctions. 3.2. Bone tissue Resorption Simulation The outcomes for correct and still left condyles grouping are given in Table 2, and for defects grouping in Table 3. Probabilities, 95% CI and 95% PI for quantification Rabbit Polyclonal to MTLR of bone defects of different sizes, simulated at different anatomic locations in the mandibular condyles, are shown in each table. Table 2 Statistical results based on the condyles. Table 3 Statistical results based on the defects. In Table 2, the column for 3CML shows the average statistics for those three defects. Statistical analysis results revealed very high probability (ranging 0.93-1) that this difference between the sample hypothesized mean (3 or 6 mm defect) was less than 0.5mm. In Table 2 all obtained values for ? = 3 or = 6), except for 3mm defects on the right condyles. All the 95% PI intervals for left and right condyles contained the hyphotesized means (Observe that PI is usually wider than CI, see Table 2). Table 3 also showed positive results, since the grouping made here was not based on the place the defect was generated (right or left condyle), but was based on the size. ? = 3, however this interval was very close to this value (2.67, 2.92). Both 95% PI contained respective hyphotesized means. 3.3. Clinical case example of longitudinal asssements of condylar resorption Patient presented in the clinic with severe open bite that developed as a sequel of marked bone resorption in the mandibular condyles between before and 2 years after jaw surgery. This patient received maxillary impaction for correction of open bite. The baseline CBCT used at age group 19.5 y demonstrated radiographic signs of generalized flattening AS-605240 and sclerosis on both right and still left condyles, confirming the radiographic diagnosis of TMJ OA. Condylar flattening was within both still left and correct condyles before medical procedures, and was more serious on the proper condyle. Condylar flattening advanced during postsurgery AS-605240 orthodontics and after treatment. Enrollment and semitransparent overlays displays condylar adjustments between pre-surgery (clear mesh lines) and recall (surface area models). Best lateral sights of jaw surface area versions from pre-surgery through recall 24 months postsurgery (12 months post orthodontics) present the general mandibular anatomy of the patient across time. Frontal views of condylar morphology prior to medical procedures through recall C. Registration and overlay of show progression of condylar changes (see Physique 8). Measurements of longitudinal changes including 3D absolute distances, signed distances and vectors of bone changes were obtained for each condyle (observe figures 9 and ?and10).10). Close up views of the longitudinal follow-up of changes in the right and left condyles. Absolute distances and.
Purpose High temperature shock protein A2 (HspA2) expression was quantitatively measured
Purpose High temperature shock protein A2 (HspA2) expression was quantitatively measured in individual testis and its own relationship using the spermatogenetic status and laboratory outcomes of intracytoplasmic sperm injection (ICSI) was investigated. hypospermatogenesis group (excluding maturation arrest due to only 1 case) are outlined in Table?1. There were no statistical variations Mouse monoclonal to CD45RA.TB100 reacts with the 220 kDa isoform A of CD45. This is clustered as CD45RA, and is expressed on naive/resting T cells and on medullart thymocytes. In comparison, CD45RO is expressed on memory/activated T cells and cortical thymocytes. CD45RA and CD45RO are useful for discriminating between naive and memory T cells in the study of the immune system in the rates of fertilization (indicates the regression collection for immunostaining intensity … Discussion Heat shock proteins (HSPs) are evolutionarily highly conserved molecular chaperones from prokaryotic bacteria to mammals, including humans to conquer an exogenous stress such as exposure to high temps [11]. Several users of HSP70 and HSP90 family Tianeptine sodium IC50 members play important tasks in cellular division and development during spermatogenesis process. The 70-kDa high temperature surprise proteins (HSP70s) are chaperones that help out with the folding, disassembly and set up of proteins complexes [12]. In mice, spermatocyte-specific hsp70-2 is normally a unique person in the mouse HSP70 family members that's developmentally and particularly portrayed in spermatogenic cells. Advanced of hsp70-2 appearance in pachytene spermatocytes was noticed through the meiotic stage of spermatogenesis. In addition, it participates in the synaptonemal complicated (SC) function during meiosis in man germ cells, and it is linked to systems that inhibit apoptosis, DNA recombination and fix procedures [13]. In hsp70-2 ?/? knockout mice model, the fat of testes of adult mice was 1 / 3 of these from wild-type mice and postmeiotic germ cells lacked in testicular tissue. However, endocrine function appeared unchanged because seminal vesicle mass was various other and regular supplementary sex features weren't been affected. This implies that hsp70-2 is essential for set up of proteins complexes necessary for conclusion of meiosis. Histological study of the mice revealed that meiotic and mitotic germ cells had been present, but postmeiotic germ spermatozoa and cells had been absent. Individual HspA2 gene is normally homologous the mouse hsp70-2 gene extremely, except for just four proteins. HspA2 additionally includes a six amino acidity sequence close to the carboxy terminal end not really within mice and rats [14]. The proteins (previously creatine kinase M isoform) [2] aswell as mRNA are portrayed significantly in individual testes with regular spermatogenesis, but repressed in testes with unusual spermatogenesis [3, 4]. Furthermore, the HspA2 gene manifestation was down-regulated in ejaculated spermatozoa from infertile males with idiopathic oligoteratozoospermia [15] and adolescents with varicocele and oligozoospermia [16]. Relating a more recent study, spermatozoal HspA2 manifestation was significantly higher in fertile compared to infertile individuals and the presence of the protein on sperm surfaces significantly increased following capacitation [17]. Actually, a growing number of researchs about HSPA2 and Tianeptine sodium IC50 fecundity was reported. Some have evaluated HSPA2 in testicular cells [3, 4, 18] while others have assessed HSPA2 manifestation in sperm [7, 15, 19, 16, 17]; some have assessed HSPA2 manifestation at RNA level [4, 16, 17] while the others at protein Tianeptine sodium IC50 [3, 7, 15, 17C20]; some are based on humankind data [3, 4, 7, 15C17, 19] while additional animal model [12, 13, 21, 18, 20]. The source of the analyte may acount for the consistent or nconsistent and even contradictory inference. However, till right now, the acknowledged HSPA2 related physiological and pathological processes can be summarized as following: rate of recurrence of chromosomal aneuploidies, DNA restoration [16, 22], cell apoptosis [13, 23], morphology and/or cytoplasmic extrusion [19], absence of histoneCprotamine exchange [23], zona binding sites on sperm surface [2, 24], fertilization and pregnancy following in vitro fertilisation (IVF) [25]. However, the studies aforementioned were primarily descriptive and/or semi-quantitative ones and neither quantitative analysis of HspA2 expression in testis nor the correlation of histological scores of spermatogenesis and intensity of HspA2 were performed till now. It is generally acknowledged that computerized image analysis plays an important role in transforming qualitative perception to quantitative characteristics, descriptive results to numerical value. Among the parameters achieved through image analyzer, integrated optical density (IOD), usually used to quantify.
MiRNAs regulate gene manifestation by binding predominantly to the 3UTR of
MiRNAs regulate gene manifestation by binding predominantly to the 3UTR of target transcripts to prevent their translation and/or induce target degradation. EBV that are associated with a number of human cancers. Right here, we briefly bring in herpesvirus-encoded Plerixafor 8HCl miRNAs and concentrate on how CLIP technology offers mainly impacted our knowledge of viral miRNAs in viral biology and pathogenesis. I. MiRNA-mediated Rules of Gene Manifestation The 1st miRNA, lin-4, was found out in 1993 in C. elegans mainly because a little RNA regulating the manifestation from the gene1. MiRNA-mediated rules of gene manifestation was initially thought to be a nematode-specific trend. This transformed in 2000 using the finding of allow-72, that was after discovered to become conserved across varieties3 quickly, and activated the seek out more miRNA-encoding genes. Since then the number of miRNAs registered at miRBase4, 5 has risen to more than 18,000 entries (release 18, Nov. 2011). MiRNAs are short (21-23 nt), non-coding RNAs that bind to partially complementary sequences mostly in the 3UTR of target transcripts to inhibit their translation and/or induce their degradation. The majority of miRNAs are processed from larger, capped and poly-adenylated RNA pol II transcripts as part of a 70-80 nt RNA stem-loop, the primary (pri)-miRNA. However, some gamma-herpesviral and a recently reported retroviral miRNA are processed from pol III transcripts6, 7. The pri-miRNA is cleaved in the nucleus by Drosha/DGCR8. This liberates a shorter stem-loop, the pre-miRNA, which is then exported Plerixafor 8HCl into the Plerixafor 8HCl cytoplasm by Exportin/Ran-GTP, where it is cleaved by Dicer, resulting in a 21-23 nt RNA duplex. Usually only one strand, the guide strand, is incorporated into the RNA-induced silencing complex (RISC) to accomplish the silencing function, while the other strand (passenger strand) is degraded. In contrast to siRNAs, miRNAs in most cases are only partially complementary to their targets. MiRNA target specificity is predominantly determined by the seed sequence, which comprises 7-8 nt at the miRNA 5 end that are fully complementary to the target8-10. Seed pairing can be supplemented by base pairing interactions at the 3end of the miRNA10. Moreover, Shin et al. showed a new class of miRNAs lacking perfect seed and 3 end pairing and binding instead with 11-12 central nucleotides11, while Chi et al. describe miRNAs binding with a G-bulge in the mRNA opposite to miRNA position five and six12. A major component of RISC is the Argonaute (Ago) protein. In mammalian cells there are four Ago proteins that are all incorporated into RISC, however, only Ago2 has endocuclease activity to cleave the target transcript13. Ago proteins contain two RNA-binding domains, the PAZ domain binding the 3 end of the mature miRNA and the PIWI domain interacting with the 5 end. Together they position the miRNA for target interaction (reviewed by Yang et al.14). In the case of full-length complementarity between miRNA and mRNA, miRNAs have a siRNA-like function leading to direct cleavage of the mRNA by AURKA Ago2. This mode of action dominates in plants15, but is very rare in animals. Here, miRNAs predominantly function by inhibiting protein translation (reviewed by Fabian & Sonenberg16) It is still a matter of debate, however, what the mechanism of translational repression is. Several studies involving micro-array experiments showed a decrease in transcript levels in response to miRNA targeting. This occurs via deadenylation of the targeted mRNA, which leads to decapping, initiating standard mRNA turnover processes17-19. However, the consequences on mRNA amounts are moderate generally, nourishing the essential proven fact that repression should be predicated on translational inhibition, which is backed with a few research displaying that RISC binding inhibits translation initiation (evaluated by Fabian et al.20). They are challenged, alternatively, by latest high-throughput techniques indicating that in most of focuses on with decreased proteins amounts also the mRNA amounts are decreased21-23. Pet miRNAs bind towards the 3UTR of mRNAs predominantly. Increasing evidence demonstrates miRNAs may also function in CDS (e.g.24-27). The second option focus on sites, nevertheless, although functional, are much less effective than 3UTR focus on sites21 generally, 22, 28, 29. Oddly enough, focus on sites for the same miRNA in the CDS and 3UTR of the transcript can easily possess synergistic results29. Although miRNA-induced adjustments in transcript levels are mostly moderate (1.5-2 fold), a recent very elegant paper Plerixafor 8HCl showed that small changes in transcript levels can lead to large changes in protein levels if a certain threshold is crossed30. II. Herpesviruses and Viral Mirnas MiRNAs have been identified in many eukaryotes, from single cell organisms like algae and amoebae to organisms all across.
Background: Systemic lupus erythematosus (SLE) is characterized by the presence of
Background: Systemic lupus erythematosus (SLE) is characterized by the presence of anti-nuclear antibodies (ANAs) in the serum of patients. investigation of serum sickness, encephalitis and autoimmune related carditis. Rats were injected once a week with either ANA positive or negative control serum or saline. Hearts were examined for initial signs of heart disease including the presence of lipid deposits, vegetation, increased ventricular thickness and a change in heart weight. Brains were examined for the presence of human CGP60474 antibody and necrotic lesions. Animals were observed for outward signs of neuropathy as well. Blood samples were taken in order to determine final circulating concentrations of IgG and monitor histamine levels. Results: Animals injected with ANA were significantly higher for lipid deposits in the heart and an increased ventricular thickness was noted. One animal even displayed Libman-Sacks endocarditis. Brains were positive for the presence of human IgG and diffuse internal lesions occurred in 80% of the ANA positive serum injected animals examined. Blood histamine levels were not significantly different, but less than controls by the finish from the experiment in fact. Bottom line: Since individual antibodies had been detected in the mind, additional research shall need to recognize which antibody combination reactions are taking place within the mind, examine cell infiltration aswell as characterize the antibodies connected with even more destructive consequences such as for example lesion formation. DRTF1 immune system complexes in the choroid plexus from the kidneys and human brain. [10] Within this scholarly research, we replaced individual serum albumin using a individual ANA positive serum control extracted from MBL International Company and analyzed Lewis rats for the current presence of antibody in the mind aswell as the current presence of debris on center valves. Components and Methods Pets and experimental groupings Feminine Lewis rats between 4 and six months of age had been obtained. Rats had been sectioned off into experimental (= 18 total) and control groupings (= 13 total) and arbitrarily numbered. Animal groupings had been the following: (1) ANA positive serum injected (exact carbon copy of ~55 products, MBL worldwide, = 10 experimental); (2) ANA positive serum and histamine CGP60474 injected (4 mg/kg IP, = 8 experimental); (3) saline injected (= 8 handles) and (4) ANA harmful control serum injected (MBL worldwide, = 5). Saline control group rats had been injected with saline for an interval CGP60474 of either 1 (= 3) or three months (= 5). At the ultimate end of just one 1 or three months, rats had been euthanized using skin tightening and or injected with Inactin 100 mg/kg IP and/or subjected to halothane in nutrient oil within a fume hood utilizing a nasal area cone; a hind limb draw check was performed to guarantee the pet was sufficiently anesthetized. Pursuing euthanasia, rat brains, kidneys and hearts were removed and examined for macroscopic lesions. The scholarly study design, strategies, techniques and protocols had been accepted per the rules by Institutional Pet Treatment Committee and Institutional Review Plank at Ball Condition School, Indiana, USA. ANA negative and positive serum CGP60474 ANA positive control serum and ANA harmful control serum had been each spin filtered and resuspended right into a relatively equal level of saline before make use of. This ANA serum can be used being a positive regular for ANA examining and shows a homogenous design for individual ANA antibodies. A poor control group was injected with ANA harmful control serum. This serum is certainly a standard harmful control serum for ANA examining (MBL International). Shots were ~55 models per animal once a week for a period of 1 1 1 month through the tail vein. Heart preparation Hearts were removed and rinsed in phosphate buffered saline (PBS) and placed in a sucrose storage answer[11] before storing in a ?80C freezer until use. Hearts were thawed, perfused with PBS and extra fluid was removed before being weighed. Pictures of hearts were taken using a Sony DSC-T200 Cybershot video camera with 8.1 Megapixels at 5 zoom. Each heart was then dissected and separated into atria and ventricular sections. A picture was taken to observe the ventricular wall thickness. The valves were then examined for indicators of verrucae with a 5 0.25 NA CP-Achromat objective using a microscope with side lighting. Preparation of samples Tissue samples frozen in optimal trimming temperature medium were sliced at 20 microns and placed on poly-d-lysine prepared slides. Slides were stored at ?22C until use. Slides had been thawed and treated with 4% paraformaldehyde on the rocker.
Objectives The co-occurrence of rheumatoid factor (RF) and anti-citrullinated protein antibodies
Objectives The co-occurrence of rheumatoid factor (RF) and anti-citrullinated protein antibodies (ACPA) is well referred to in rheumatoid arthritis (RA). compared to ACPA-ICs alone (P=0.003) Conclusions The combined presence of ACPA and IgM RF mediates increased proinflammatory cytokine production analyses performed on RA samples. All patients satisfied 1987 American College of Rheumatology (ACR) classification criteria for RA 17 and provided informed written consent and HIPAA authorization. Subjects included in this study include a cohort of 1 1,488 Veterans with RA (89% male), and detailed demographics are presented in Table 1. Banked serum was available for a representative population of 1 1,466 subjects and was used for multiplex cytokine and autoantibody analysis. Table 1 Characteristics of RA Research Individuals at the proper period of Research Enrollment. Furthermore to banked sera, VARA gathers medical data including baseline and longitudinal DAS28 ratings, aswell as baseline dimension of ACPA using the next era CCP2 antibody ELISA (Diastat, Axis-Shield Diagnostics Ltd., MK-0752 UK, positivity 5 U/ml). VARA also gathers RF check (positivity 15 IU/ml) and high level of sensitivity C-reactive proteins (hs-CRP) both as dependant on nephelometry (Siemens Health care Diagnostics, Germany). Hs-CRP concentrations weren't offered by follow-up appointments. While VARA can be a multicenter task, standardized autoantibodies are assessed in one investigator's lab (GMT). HLA-DRB1 genotyping MK-0752 was conducted as described18. Individuals were categorized to be bad or positive for HLA-DRB1 shared epitope-containing alleles and HLA-DR3 alleles. Follow-up measures consist of tender and inflamed joint matters (0-28), erythrocyte sedimentation price (ESR, mm/h), discomfort (0-10), multidimensional Wellness Evaluation Questionnaire (MD-HAQ, 0-3) 19, individual and service provider global assessments (0-100), and remedies. A comorbidity count number (0-9) was determined for each individual using administrative rules 20. Because we had been looking into the association of autoantibodies with follow-up scientific parameters, patients had been excluded if: autoantibody or 28-joint Disease Activity Rating (DAS28) data was unavailable; just a single scientific observation was documented; or if the full total follow-up length was < six months. Multiplex cytokine evaluation Multiplex evaluation of cytokines and chemokines in individual serum was performed utilizing the 17 cytokine Bio-Plex ProHuman Cytokine Assay (BioRad) operate on the Luminex 200 program based on the manufacturer's guidelines other than a proprietary BioRad assay dilution buffer was customized to include reagents proven to decrease the ramifications of heterophilic antibodies in multiplex immunoassays as previously referred to 21. Data digesting was performed through the use of Bio-Plex Supervisor 5.0, and analyte concentrations (in picograms per milliliter) had been interpolated from regular curves. Multiplex ACPA antigen arrays Antibodies concentrating on 37 putative rheumatoid arthritis-associated autoantigens had been measured utilizing a custom made bead-based immunoassay in the BioPlex system as previously referred to 21 22. From the 37 antigens, 30 are citrullinated and 7 are indigenous (indigenous histone 2A, histone 2B, ApoA1, filaggrin 48-65 peptide, vimentin, fibrinogen, and ApoA1 231-248 peptide). Quickly, serum was diluted and blended with spectrally specific florescent beads conjugated with putative rheumatoid arthritis-associated autoantigens accompanied by incubation with anti-human phycoerythrin antibody and evaluation on the Luminex 200 device. Data evaluation Patients were grouped into subgroups including double-negative (aCCP-/RF-; RF activity isolated from sufferers with blended cryoglobulinemia were supplied by MK-0752 Dr generously. Mariana Newkirk (McGill College or university, Montreal, Canada). The purified RA-IgG and monoclonal RF had been separately focused by centrifugation more than a 100 kD molecular pounds filtration system column with buffer exchange to PBS MK-0752 (Amicon Ultra; Millipore) and had been depleted of endotoxin by purification through a polymyxin B column (Pierce Detoxigel). MDA1 RF and RA-IgG IgM concentrations had been approximated regarding to optical thickness at 280 nm, had been aliquoted, and kept at -80C. For era of cFb-IC, flat-bottomed 96-well lifestyle plates were covered right MK-0752 away at 4C with 50 l of cFb (20 g/ml), cleaned in PBS formulated with 0.05% Tween 20, and incubated for 2 hours at 4C with 100 l of rabbit polyclonal anti-fibrinogen antibody (50 ug/ml), 100 ul of anti-cFbCpositive IgG (10 mg/ml), or, 100 ul of anti-cFb-positive IgG preincubated with monoclonal IgM RF (stock concentration 5 mg/ml used at 1:20 or 1:100 dilution) or, being a control, with PBS alone. Wells were washed in PBS containing 0 again.05% Tween 20, and macrophages (50,000/well) in 200 l RMPI containing 5% FCS were.
<. been qualified to receive the PsA-TT marketing campaign. A-769662 Most
<. been qualified to receive the PsA-TT marketing campaign. A-769662 Most of them offered info on PsA-TT vaccination position; 455 (95%) got resided in Bobo-Dioulasso and 472 (98%) in Burkina Faso during 2010. Two individuals aged 4 and 17 years reported intermittent residency outside Burkina Faso no PsA-TT vaccination. Of PsA-TT eligible participants, 415 recalled receiving MenAfriVac or a meningitis vaccine received during a campaign in 2010 2010, impartial of document confirmation. PsA-TT coverage was 86.3% (95% confidence interval [CI], 82.7%C89.9%) among those vaccine-eligible individuals who were resident in Bobo-Dioulasso during 2010 (88.8% [95% CI, 85.6%C92.0%]) compared with those who lived elsewhere that year (n = 24; 44.0% [95% CI, 16.9%C71.1%]). PsA-TT vaccination was document-confirmed for 255 eligible residents (56%) and 4 nonresidents (16%). None of the 7 persons indicating 2010 residence outside Burkina Faso reported vaccination. PsA-TT vaccination was reported or confirmed by vaccination card for 5 and 2, respectively, of the 42 children aged 11C22 months (11.9% and 7.1%, respectively), who had been born but were too young to be eligible during the 2010 campaign. Vaccination coverage did not vary substantially by age group (Physique ?(Figure1).1). Recall- or document-based vaccination among all participants was lowest among children aged 23C59 months (80.0%) and highest among children aged 5C9 years (94.9%). Approximately 60% of participants remembered specifically receiving the MenAfriVac vaccine, with little variation by age, whereas document-confirmed vaccination declined from 71.6% among 5- to 9-year-old children to 31.3% among 25-year-old adults. Physique 1. Age-specific vaccination coverage with meningococcal group A conjugate vaccine in Bobo-Dioulasso, Burkina Faso, 2011. Estimates are given for all those study participants, and specifically for participants who were residents of Bobo-Dioulasso in 2010 2010 according ... Seroprevalence Among the 481 participants aged 23 months to 29 years, the SBAref geometric mean titer (GMT) was 1939 (95% CI, 1700C2212), the SBA3125 GMT was 375 (95% CI, 261C538), and the anti-MenA IgG geometric mean concentration was 28.12 g/mL (95% CI, 21.76C32.80 g/mL). Prevalence of SBAref 128 was 97.3% (95% CI, 95.9%C98.7%); of SBAref 1024, 83.4% (95% CI, 80.0%C86.8%); LAT of SBA3125 128, 83.6% (95% CI, 77.6%C89.7%); and of IgG 2 g/mL, 84.2% (95% CI, 78.7%C89.7%). Seroprevalence of SBAref 128 did not vary A-769662 by age, whereas prevalence of SBAref 1024 and SBA3125 128 was close to 100% among 5- to 19-year-olds and as low as 65% among 23- to 59-month-olds and 20- to 29-year-olds (Physique ?(Figure2).2). Prevalence of IgG 2 g/mL increased by age, from 59% among <5-year-olds to 100% among adults. Physique 2. Seroprevalence according to different serologic outcomes among the population aged 23 months to 29 years, by age group, Bobo-Dioulasso, Burkina Faso, 2011. Included are all 481 study individuals who had been qualified to receive meningococcal group A conjugate vaccination ... All GMT and seroprevalence estimates in participants with documented PsA-TT vaccination were higher than in the entire population (Table ?(Table1).1). The association (OR) between vaccination and SBAref 1024 was 2.20 (95% CI, .29C16.84) among 11- to 22-month-old children, 1.48 (95% CI, .36C3.49) for those aged 23C59 months, 3.89 (95% CI, .70C21.72) for children aged 5C14 years, and 1.73 (95% CI, .57C5.29) for participants aged 15C29 years. Too few samples were tested for SBA3125 and IgG for statistical analyses. Table 1. Immune Status in the General Populace of Bobo-Dioulasso, Burkina Faso, 2011, by Age Group Compared A-769662 with the population aged 23 months to 29 years of Bobo-Dioulasso during 2008, geometric mean titers of SBAref were 7.59-fold higher during 2011, 51.88-fold higher for SBA3125, and 10.56-fold higher for IgG. Seroprevalence of SBAref 128 and SBAref 1024 was 1.23-fold and 1.94-fold higher, respectively, during 2011, 3.92-fold higher for SBA3125, and 1.18-fold higher for IgG. For all those serologic outcomes (geometric means or A-769662 seroprevalence of SBAref, SBA3125, and IgG) among participants aged 23 months to 29 years, values were higher among 2011 participants with documented PsA-TT vaccination compared with 2008 participants (Table ?(Table11). Among vaccinated persons (document-based), the correlation between SBAref and SBA3125 titers was strong among children aged 23 months to 4 years ( = 0.81, < .001), and moderate for 5- to 14-year-olds ( = A-769662 0.66, < .001) and 15- to 29-year-olds ( = 0.62, = .001). IgG concentrations correlated poorly with SBAref titers, whereas they.


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Explore the beautiful fiord of Milford Sound from Queenstown or Te Anau on this excellent-value full day tour!
Departing from Queenstown, you'll travel in style aboard a luxury glass roof coach with breathtaking views. Once you arrive at Milford Sound, you'll transfer to a modern, uncrowded catamaran to begin your cruise. Be spoilt with unlimited, close-up viewing of waterfalls, wildlife and rainforest from three viewing decks. Take amazing pictures from any of the decks (both indoor and outdoor) during the 1 hour 45 minute cruise around one of the most stunning places in the world! Throughout the cruise, you'll hear informative commentary from the crew. You may even be lucky enough to witness Bottlenose Dolphins playing in the boat's wake so keep your camera handy. You'll also enjoy a complimentary and tasty lunch.
It rains 180 days a year in Milford Sound and when it does, torrents of water will pour into the fiord from thousands of temporary waterfalls. This is the ideal time to be in Milford! If you're fortunate, you'll enjoy rain and sunshine together which can create the most beautiful rainbows.
Come on a magical full day adventure to Milford Sound – book your spot online with us today!
Departing from Queenstown:
Transfers are available for certain hotels, please enter your hotel name when booking.
7:35 am departure (May to September)
7:25 am departure (October to April)
Tour duration: approximately 12 hours
Departing from Te Anau:
Meeting point: Kiwi Country Cafe, Miro Street, Te Anau
Please arrive at least 15 minutes prior to the departure time.
9:40 am departure (October to April)
9:50 am departure (May 2020 to September 2020)
Tour duration: approximately 7 hours
Child ticket: Ages 5 - 14 years
Infants under 5 are free, but must travel on the lap of an adult.
This cruise can carry up to 200 passengers.
What to bring:
Warm clothing and a rain jacket (the weather can be unpredictable all year round and Milford Sound is one of the wettest places in New Zealand)
Non-slip shoes/boots
Cash/credit card for purchases on-board
Seasickness medication (if you are prone to getting seasick)
Upon booking this product, you will receive confirmation in the form of a tour voucher.
Please present the voucher on your mobile device on the day of the tour.
Your tour operator's contact details are included on the tour voucher.

subset: commoncrawl


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Cancer is one of the most agonizing illnesses that affects the human body . Yet, despite the huge amount of funding poured into research globally, scientists have still to come up with a satisfactory explanation with regard to how it is caused. Compounding matters further, is the fact that there are different cancers located in different sites in the human body, each having its own unique symptoms making a general one shot treatment difficult.
While awareness about cancer today is much more than it was a decade ago, following education programs conducted by the National Cancer Prevention Program ( NCPP) and the Non Communicable Diseases Unit of the Health Ministry, not many patients take advantage of the services provided to them free of charge, to have themselves screened regularly, especially, if they fall into the high risk category.
The Sunday Observer spoke to Oncologist and Community Physician, National Cancer Prevention Program (NCCP) Dr Suraj Perera to give us more insights into this disease as Sri Lanka moves towards the UN goal of a cancer free world backed by President Maithrapala Sirisena and Health Minister Dr Rajitha Senaratne.
Q. On February 4 ( World Cancer Awareness Day) the world came together to make its collective commitment to eradicate a disease that continues to remain elusive to cure, preventing any significant forward strides until scientists are able to resolve the root causes. As many persons including patients still do not understand what this complex disease is all about, explain what Cancer is, in laymen language .
A. Cancer is an un-controlled, purposeless growth of abnormal cells in the body, which can occur at any place in the body. These cells have the potential to invade adjacent tissues and migrate to a distant body site through blood or lymph vessels, and grow in new sites. This process is called 'metastasis'
Q. Are there different types of Cancer? If so what are they?
A. 'Cancer' is a generic term for a large group of diseases that can affect any part of the body. Therefore, cancer is not a single disease. There are more than 200 types of cancers, and they can be grouped according to the type of cell they start. Cancers can be grouped to 5 main categories.
(i) Carcinoma- cancer that begins in the skin or in tissues that line or cover internal organs
(ii) Sarcoma- cancer that begins in the connective tissue or supportive tissues
(iii) Leukaemias – cancer that starts in blood forming tissues.
(iv) Lymphoma and myeloma – cancer that begins in the cells of immune system.
(v) Brain and spinal cord cancers – cancers that begins at the central nervous system
Q. Are tumours different from cancer? I understand there are different groups of tumours . Can having a tumour lead to cancer?
A. Tumour is a growth or lump in the body. It can be cancerous (malignant) or not (benign). Therefore all tumours are not malignant.
Q. How is cancer caused?
A. The smallest unit of the human body is a cell. Inside almost every cell there is a central structure called 'nucleus'. In the nucleus, there are 23 pairs of chromosomes.
These chromosomes are made up of 'genes.' Most cancers start due to gene changes that happen over a person's lifetime due to natural process or due to the effect of external factors such as tobacco smoke, radiation, chemical in the environment, etc. These changes of genes are called 'mutations'. Rarely cancers start due to inherited faulty genes passed down in families.
Q. What are the first signs and symptoms of the onset of cancer? Are they specific to each type of cancer?
A. At the onset of cancer there may be no symptoms or signs. When cancer spreads the symptom may appear gradually. Symptoms and signs may vary according to the site of the cancer.
Q. Who are those most at risk of getting it – age wise ?
A. With advancing age, the risk of cancer increases. According to the data of 2014, the highest incidence of cancers are reported in the age group of 70-74 (crude incidence rate is 707/100,000 population).
Q. Yet today we see younger people being struck by the disease. Why?
A. Even though limited numbers of cancers are reported at younger age groups, there is little increase incidence of cancers over the years among younger age groups
Q. What are the tests needed to identify a malignant cancer ? Do they vary from cancer to cancer e.g breast cancer, cervical cancer?
A. According to the site of cancer , the investigations may vary. See table 1
Q. Are these tests available in all State hospitals with cancer clinics in Sri Lanka? Where ?
A. Mammograpy machines – National Hospital, Teaching Hospitals – Kandy, Peradeniya, Karapitiya, Anuradhapura, Batticaloa, Ratnapura, Jaffna, Apeksha Hospital Provincial General Hospital – Badulla, Kurunegala District General Hospital – Polonnaruwa Colposcopy facilities - Castle Street Hospital for women, Apeksha Hospital, TH Kandy, TH Mahamodara, TH Hospital Ratnapura, DGH Kegalle, Base Hospital Telippalai, TH Batticaloa, TH Jaffna
Q. Where are these facilities available in Sri Lanka?
A. Cervical cancer – screening program is for all married /sexually active females from the age of 35 . Services are available in more than 1,000 Well Woman Clinics island wide. It is also available daily at the Cancer Early Detection Centre, Narahenpitiya, and De Soysa Maternity Hospital . Some of the gynaecological units and the private sector also offer this service.
Oral cancer – Early detection of oral potentially malignant disorders ( OPMD) through screening of persons with high risk behaviours (consuming betal quids /alcohol/smoking/tobacco or arecanut related products. Clinical oral examination for oral cancer /OPMD is conducted at all government dental surgical clinics.
Q. Can treatment cure certain cancers? If so what are they?
A. Yes. If a person with suspicious symptom or sign comes to a health institution as early as possible, medical teams can direct them to necessary investigations to confirm or exclude having a cancer.
If cancer is confirmed, with timely initiation and completion of treatment some cancers can be cured successfully, (Eg. breast cancer, thyroid cancer, childhood leukaemia etc)
Q. Cancer places a heavy economic burden on the family and carers and state aid is often not enough for the underprivileged. What has the National Cancer Control Program (NCCP) done to help these persons?
A. Patients are encouraged to obtain financial support through the Social Services Officers attached to Divisional Secretariat Offices.
Additionally several non governmental organizations work with cancer centres to provide financial and material support for cancer patients. Also, the National Cancer Control Program gives necessary guidance to NGOs and religious organizations to help needy families with cancer. Even community based organizations may be able to assist cancer patients.
Q. From where can patients get details of such services?
A. Cancer patients can always ask medical and nursing staff at cancer centres about it and they will direct them.
Q. Treatment wise what is the first line of treatment for someone who comes to your clinic with a suspected lump of some sign of cancer?
A. Those who are presented with suspected lesions may need to undergo diagnostic tests to confirm or exclude cancer.
bSee table 2
Q.Is this procedure followed in all state hospitals?
A. Yes these services are available at Base Hospitals, District General Hospitals, Provincial General Hospitals and Teaching Hospitals
Q. Is radiation and chemotherapy the standard treatments? Is it given to children and adults as well?
A. Yes. Surgery, chemotherapy and radio therapy are the standard treatment for most of the cancers.
Q I understand the Health Ministry has introduced some in-service training programs and diploma courses for new medical students and nurses. Tell us more about them.
A. The first batch of medical officers now follow the Post Graduate Diploma in Palliative Medicine . In addition, a curriculum is being prepared for Post Basic Diploma in Palliative Nursing.
Q. The NCCP is the focal point of cancer services. What are the services you offer at Narahenpitiya? I understand you have walking mobile services?
A. Consultants, medical officers, dental surgeons, public health nursing sisters and nursing officers work together as a team providing interventions for primary prevention of cancers, early detection services (cervical cancer screening through pap smear test and HPV test, and clinical breast examination. The centre is open from 8 a.m. to 4p.m.
Q. The World Campaign theme for this year "I am" and "I will," marks the launch of a three year call to action ending in 2021 to empower cancer patients and raise awareness. What are the programs that the NCCP will be conducting to this end during this period?
A. Through this theme, every person is empowered to continue to practise healthy habits and ensure a safe environment for primary prevention of cancers, encourage every person to be vigilant for early warning symptoms of cancers and promote participation in healthy lifestyle centres and Well Women Clinics at recommended age groups.
Also those diagnosed with cancers need to be empowered to continue their specific treatment.
Q. Cancer patients are still constrained by prejudices and myths. Some prefer to go to kattadiyas and do home treatments like drinking papaw juice and katu atha before seeing a Western qualified doctor. Your comments?
A. These practices need to be discouraged. The public need to be educated to seek evidence based treatment options.
Q. Your message to the public?
A. One third of cancers can be prevented through healthy lifestyles and safe environments. Another one third can be cured successfully through early detection and prompt treatment.
The balance one third can be managed through treatment, survivorship care and palliative care and ensure the quality of life.


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Do you like walking around in public listening to music? It's a good way to block out the unwanted hustle and bustle, but there are some dangers and disadvantages you should be aware of. You should be street smart when it comes to using headphones.
1Get the right headphones. Headphones of a lower quality will cause you to turn your music up loud in order to hear it well. Headphones of a higher quality will allow you to lower the volume. This will allow you to hear the important things that are going on around you in public spaces.
2Don't turn your headphones up too loud. Not only is this bad for your ears, it completely blocks out all sounds around you leaving you unable hear important things like people yelling after you or cars beeping at you. This increases the risk of something dangerous happening to you or being caused by you.
3Keep your musical device in your pocket, especially if it is a smart phone or iPod. These devices can easily snatched away from you by opportunistic thieves and are very expensive to replace. If you like to switch your songs a lot, make a playlist and switch your songs that way.
4Respond when someone taps you. Look at them and acknowledge that they are there. If they are a stranger, say, "Hi" to them very kindly and avoid eye contact if having a conversation with them concerns you. If they persist in touching you, kindly tell them that you're not interested, and put your earphones into your pockets. This way they know you are fully aware that they are following you.
5Walk fast while you listen to music. It may seem silly, but it looks like you are on an evening jog rather than trying to get through an urban neighborhood without trouble.
6Know your route. If you don't know where you are going, and are in a neighborhood that seems dangerous, take your headphones out to let locals know that while you may be lost, you are not too confused as to your surroundings.
7Look at shadows on the ground. If you see one that is bigger than yours, turn your head behind to check and see if anyone is there.
8Look behind you every time you turn a corner to make sure no one is following you. Often times, people think because you are listening to music you are completely oblivious of your surroundings. Make sure that every time you turn a corner there is no one behind you, and that people you've seen before aren't there anymore.
9Ignore people when they yell at you. A lot of individuals often find it funny to intimidate the public. When you ignore them, it makes them feel insecure, and they end up looking stupid. Just jam to your music and enjoy your day!Advertisement
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- Take your earphones out while walking to your house. This makes you less vulnerable while walking home.
- Looking behind yourself lets others know you are aware they are behind you.
- Keep your headphones in a safe place at home to avoid losing them.
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Harding Park Golf Course
This afternoon we left WWDC early and headed out to Harding Park Golf Course in San Francisco for a twilight round. The non-resident rates have been increased significantly since the American Express World Championships were held there in 2005, so we elected to wait until 5pm when the cheaper rates kicked in. We ate at the new clubhouse before the round, where I ordered fish and chips that were hot and fresh.
I was a little rusty out of the gate, but had a chip-in birdie later in the round and finished with an 89. We played the 18th hole in the dark, but did get the round in by hustling at the end and pushing the slower groups in front of us.
I felt like a superstar after the round–the driver our hotel arranged for us arrived right on time in a shiny black Mercedes. He was very polite (calling me "sir" is just too cool) and had bottles of water for us. Great end to a great afternoon of golf.Originally published by DK on June 14, 2007 at 1:29 am


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Muriel J. Thorp, age 95 of Glenwood, died Friday, June 08, 2018 at the Glenwood Retirement Village. A memorial service celebrating her life will be on Monday, June 18, 2018 at 11:00 A.M. at Glenwood Lutheran Church. Inurnment will be at a later date in Fort Snelling National Cemetery.
Muriel J. Thorp, the daughter of Carl and Elsie (Plack) Irgens was born June 13, 1922 in Minneapolis, Minnesota. She was baptized, confirmed and married at St. Paul's Lutheran Church in Minneapolis, where she was also active in the choir and taught Sunday school. On April 12, 1944, Muriel was united in marriage to Carl Thorp. The couple made their home in Minneapolis and were blessed with six children. They shared 73 year of marriage before Carl passed away October 14, 2017
Muriel joined the Minnehaha Chapter #37 of the Eastern Star in 1943; where she served as Worthy Matron in 1974. In 2001, when she moved to Glenwood, she joined the Minnewaska Chapter #129 of the Eastern Star. Along with being active in the Eastern Star for over 75 years, Muriel was Guardian of Jobs Daughters and a Den Mother for 9 years. She enjoyed crafting, playing cards, games and was a farkle champion! Muriel especially loved traveling with her husband in their R.V. and spending time with her children, grandchildren and great-grandchildren. She will be remembered as a wonderfully patient and caring mother and homemaker. Muriel passed away June 8, 2018; her last days were spent surrounded by her family and the caring staff at the Glenwood Retirement Village. Muriel had pre-planned her service and when writing her obituary she included that she "enjoyed life a lot".
She is survived by her children: Dennis (Barb) Thorp of Oklahoma, Terry Thorp of Minnetonka, Donald (Bonnie) Thorp of San Diego, CA, Marcia (Gary) Wold of Glenwood and Karen (Terry) Lund of Eagan; 11 grandchildren; 17 great-grandchildren; nieces, nephews, other relatives and friends.
Muriel is preceded in death by her parents; husband; daughter, Cathy Thorp; grandson, Michael Thorp; sister and brother-in-law, Shirley and Wilbur Goetz; and nieces, Carol and Susan Goetz.
To send flowers to the family or plant a tree in memory of Muriel J. (Irgens) Thorp, please visit our floral store.


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Friday 5th Week of Lent
The Gospel Today
The Jews then picked up stones to throw at him; so Jesus said, "I have openly done many good works among you, which the Father gave me to do. For which of these do you stone me?"
The Jews answered, "We are not stoning you for doing a good work, but for insulting God; you are only a man, and you make yourself God."
Then Jesus replied, "Is this not written in your law: I said, you are gods? So those who received this word of God were called gods, and the Scripture is always true. What then should be said of the one anointed, and sent into the world, by the Father? Am I insulting God when I say: 'I am the Son of God'? If I am not doing the works of my Father, do not believe me. But if I do them, even if you have no faith in me, believe because of the works I do; and know that the Father is in me, and I in the Father."
Even if you have no faith in me, believe because of the works I do.
Again they tried to arrest him, but Jesus escaped from their hands. He went away again to the other side of the Jordan, to the place where John had baptised, and there he stayed.
Many people came to Jesus, and said, "John worked no miracles, but he spoke about you, and everything he said was true." And many in that place became believers.
First Reading Jeremiah 20:10-13
Psalm 18:6
- "In my distress I called upon the Lord and he heard my voice."
Saint of the Day, 7 April
St John Baptist de la Salle, priest


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Young and the Restless Recap Thursday, January 10: Phyllis Interrupts JT Memories, She Has Something That Needs To Be Heard
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"Young and the Restless" (YR) recap for Thursday, January 10, 2019, reveals that over at Victoria Newman's (Amelia Heinle) place, Reed Hellstrom (Tristan Lake Leabu) and Billy Abbott (Jason Thompson) set up for J.T. Hellstrom's (Thad Luckinbill) memorial.
Y&R Spoilers JT's Memorial Gets Under Way
Nikki Newman (Melody Thomas Scott) arrives and Reed is dispatched to get herbal tea. When Billy steps outside, Victoria questions Nikki attending; she's been through enough. Nikki doesn't want to abandon Reed and knows she's the reason he's going through this. Billy reappears as Charlie Ashby (Noah Alexander), Mattie Ashby (Lexie Stevenson), and Cane Ashby (Daniel Goddard) arrive. Mattie talks to Billy about Lily Ashby (Christel Khalil). Sharon McAvoy (Sharon Case) arrives. Victoria asks Cane why he felt the need to come and says it's difficult enough. Billy interrupts so Victoria joins Sharon.
Young and The Restless Spoilers Phyllis Decides To Attend The Memorial
At home, Phyllis Summers (Gina Tognoni) doesn't plan to attend J.T. Hellstrom's memorial she thinks he was a horrible person and she can't understand how Victoria's doing this. Nick Newman (Joshua Morrow) points out he's going for Reed Hellstrom, who lost his father. Phyllis feels Reed won't miss him, and she shouldn't be around Victoria Newman because she might haul off and slap her right back. Nick mentions Nikki Newman's going and Phyllis thinks that will be stressful. She decides to come after all.
False charges force a shocking confession this week on #YR. Don't miss it! pic.twitter.com/xdlyZSWFxI
— Young and Restless (@YandR_CBS) January 6, 2019
Y&R Spoilers Kyle Gushes To Traci
At the Abbott mansion, Kyle Abbott (Michael Mealor) and Traci Abbott (Beth Maitland) discuss the memorial. She hopes it will be about more than J.T. Hellstrom's troublesome ending and knows many people cared about him. Kyle reveals Lola Rosales (Sasha Calle) invited him to the Club and they're in love. Traci is happy for him. She enthuses that he's making memories that will last forever.
Young and The Restless Rey Gets A Call About A Drug Bust
In the apartment, Mia Rosales (Noemi Gonzalez) finishes Lola Rosales' make up. Lola declares it perfect and Rey Rosales (Jordi Vilasuso) declares her a beautiful, elegant woman. Lola explains she's taking Kyle Abbott on a date and tonight she'll show him how special he is. After, Rey thinks Kyle won't last long, but Mia disagrees because Lola's in love. Rey gets called for a drug bust and says he'll call Sharon McAvoy about the broken furnace. Mia pushes him to go and says she'll take care of it.
Y&R Arturo Surprised Abby's Going To The Memorial
At Dark Horse, Abby Newman (Melissa Ordway) declines Arturo Rosales' (Jason Canela) invitation to his place; she's going to J.T. Hellstrom's memorial. Arturo's surprised, given her father's been arrested for his murder. Abby concedes it will be uncomfortable. After Abby leaves, Mia Rosales calls Arturo to fix her furnace.
Traci's speech is exactly what Reed needed. 👏 #YR pic.twitter.com/eAX4iRHe6S
— Young and Restless (@YandR_CBS) January 10, 2019
Young and The Restless Reed Touched By Traci's Comments
Traci Abbott arrives at Victoria Newman's house and is greeted by Billy Abbott and Reed Hellstrom. She tells the teen he reminds her of the best parts of his father. Reed thinks it's nice of her to say J.T. Hellstrom had good parts. Traci embraces him. Next, Mackenzie Hellstrom (Kelly Kruger) arrives. Reed says it wouldn't be the same without her there. Mackenzie knows it's hard to make peace with J.T.'s death when there are still so many questions.
Y&R Phyllis Thinks Nikki Being At The Memorial is A Mistake
Elsewhere, Sharon McAvoy watches as Abby Newman and Nikki Newman hug over Victor Newman (Eric Braeden). Nick Newman and Phyllis Summers arrive. Phyllis pulls Victoria aside to complain about Nikki being under too much pressure and thinks they need to get her out of there. Victoria will only be throwing out Phyllis. Nikki and Sharon join them, and Nikki can't promise the truth won't come out. Phyllis asks, "What does that mean?"
This is a tough time for Nikki. ❤ #YR pic.twitter.com/reC95MyGAC
— Young and Restless (@YandR_CBS) January 10, 2019
Young and The Restless Rey Calls Sharon For Help On A Case
After Mackenzie spots, Nick with Phyllis and Cane Ashby updates her on Billy Abbott and Phyllis' split. Mackenzie watches Billy with Victoria and looks thoughtful and so does Phyllis. She takes him aside to ask if they're getting back together. Sharon takes a call from Rey Rosales who needs help with a child at the station and leaves.
Y&R Guests Start Talking About JT Memories
Reed begins and asks to hear good memories of his dad. Traci goes first, she recalls J.T.'s love for Colleen, his musical talent, and hopes he's with her daughter now and has put the darkness behind him. Reed talks about J.T. and gets choked up recalling when they played guitar together and sobs over missing him. Mackenzie steps forward to say that's the man she will remember. Nikki cries, "Oh Reed, I'm so sorry." Phyllis jumps up and says she has something to say about J.T. that needs to be heard.
Find someone who looks at you the way Kyle looks at Lola. 😍 #YR pic.twitter.com/FTWFi53iOG
— Young and Restless (@YandR_CBS) January 10, 2019
Young and The Restless Lola Prepares A Special Evening For Kyle
At the Athletic Club, Kyle Abbott tells Lola Rosales she looks amazing. She reveals she's living out a romantic fantasy. They kiss, slow dance, and laughingly talk about the assumptions they made about one another initially. Kyle remarks that they skipped all the drama and says being with her is so damn easy. He had no clue this was possible and realizes it's how it's supposed to be when you're in love. Lola thanks him for being so great about waiting for sex. Kyle reassures her but looks disappointed.
Y&R Arturo Arrives To Fix Mia's Furnace
Arturo Rosales arrives at Rey Rosales apartment and declares Mia Rosales' furnace has had it. She wonders what to do. She is freezing. Arturo advises that she's resourceful, she'll figure something out. Later, Mia's shaking beneath a comforter when Arturo returns with news his buddy can bring a unit by after work. Mia convinces him to join her under the comforter to wait. They get close looking at photos of Lola Rosales. Mia talks about Rey Rosales' absence and for some reason tonight it's getting to her.
And that's a wrap. Don't forget to check often with Soap Opera Spy for all your soap needs!
As always, more detailed spoilers and a full collection of pictures for the week in question will appear here on Soap Opera Spy, where there will be more clues and hints over exactly what's going on.
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James LePore is currently playing Harry Roat in The Newtowne Player's production of "Wait Until Dark", running through Sunday, October 21st.
Bio: James LePore (Harry Roat), grew up on Long Island, NY, and has been a self-proclaimed TV, film, stage, and radio dreamer his entire life. After moving to Southern Maryland in 2006, he got his start as an actor in a local Ghost Tour and was hooked. 10 years later, he's not only appeared in over a dozen stage productions, but also became a radio DJ, hosted a weekly TV show in Southern Maryland called "The Buzz", and now produces his own version of the TV show on YouTube ("What's the Buzz with James LePore"). He has certainly been given many opportunities to live the dream, and lives every minute looking forward to meeting new friends and learning new things. He'd like to thank all of those talented and wonderful people who have grown him and pushed him to chase his dreams.
For more information about The Newtowne Player's production, "Wait Until Dark", click here.
How is the character you play, Harry Roat, similar/different than who you are in real life?
Wow, talk about contrasts. James LePore is a big puddle of emotion. I believe in romance, giant hugs that last a full minute, crying at movies or songs that hit a particular emotional chord, and I'm not shy about telling people that mean something to me how important they are. I start every day with gratitude. My Feng Shui number is 3, or "bringer of light," and I take that very seriously every day. Harry Roat, on the other hand, is a sociopath. He sees the goal – money – and does not see any moral problems with doing whatever needs to be done to get there, including but not limited to, murder, torture, sadism, and who knows what else. As an actor – reaching inward for whatever dark stuff the back of my mind can generate, and letting it spill out into the world safely, is deliciously satisfying. When we get shrieks from our audience, it is so hard to stifle a smile and a chuckle of satisfaction for a job well done. That job well done is the product of the creative genius that is my director, DJ Lavery, who has made this show the gasp! that it is. I'd work for him again even if he was directing the phone book. Harry Roat is also the product of long hours of playing against some of the best actors and actresses (sorry, I'm old school) I've ever worked with. Each one's reaction/interaction with Harry helped me to more finely hone who he is. I would NOT ever want to cross paths with this man in real life, but the audience can, safely, under controlled conditions. He's like an unpredictable animal let out of a cage.
If you had to choose 5 words to describe "Wait Until Dark," what words would you choose and why?
Tension. Darkness. Silence. Unpredictable. Engaging.
I say "silence" because sometimes the silence in the dark is even scarier than what you can hear in the dark. Audiences will see and hear what I mean.
Did you audition specifically for this role, or did you have another one in mind?
Oh, no, Harry was my goal. I'd been a longtime fan of the 1967 film with Audrey Hepburn and Alan Arkin in the role I play. It scared me as a kid, and while the movie itself may be tame to today's audiences, our creative team who also includes Chris Maulden as Assistant Director and Stage Manager Megan Rankin Herring, has found ways to revive the scares and bring the claustrophobic fear of a woman struggling without sight to evade a predator confined in a small space, well, it's really remarkable. And you will jump. Shrieks are possible, too. It happens every night. In his book, Danse Macabre, Stephen King writes, "WAIT UNTIL DARK is the scariest movie of all time" and that Alan Arkin's performance "may be the greatest evocation of screen villainy ever". How could I not want that role?
What is your favorite part of "Wait Until Dark"?
I don't think I'd being giving anything away to say the last scene – almost everyone knows it will be a final, climactic battle in the dark with Susy, the blind woman, but there is a MOMENT in that scene where I get to deliver a line that is so sadistic, so controlling, so evil – it's just my favorite line by far. If I were given a second choice, it would have to be my last 4 words in the play. During the initial read-through, it gave a few people chills. I'm surprised they still wanted to work with me. Haha.
How did you go about becoming Harry Roat?
I watched Alan Arkin's performance, to be sure, but I also watched Stacey Keach play the same in a 1982 made for TV movie, and watched several clips of Harry Roat on YouTube played by community theatre actors, looking to see what I liked and what I didn't like. I looked up sociopaths on the internet and thought about how one would act if all moral influences were removed. Certainly my director had everything from overreaching ideas to minute fine-tuning, and so did my castmates. My best advice as an actor; never stop listening. I can't tell you how many times my fellow actors would tell me that one particular thing was great or something wasn't working, and it tipped the balance scale of Harry one way or another, so to speak. Creative people feed off one another, and that's the way it should be. Nobody knows everything, especially in the intangible world of the performing arts. There's shades of improvement to be had everywhere. I thank my directors and cast-mates – Stephanie Diane Taubert, Michael Sokoloff, Kenny Faison, Eva Reynolds, Madysen Maulden, Thom Esposito, Mike Culbreath, and Steve Fogle for providing me with ideas on those shades. It's a team effort. Come see for yourself, then say hi to us in the lobby after the show. I mean, you know, when your hands stop shaking.


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La Linea Verde have introduced new ready-to-eat products, which were showcased at the Marca fair, in Bologna. These products are almost 10 different vegetables cooked in three different manners and packaged with the 'skin' technique.
Andrea Battagliola, a member of the marketing management board of La Linea Verde, explained, "The vegetables are steam-cooked, grilled and baked. These products are arousing much curiosity and they are also very appreciated, considering the tests we conducted. We wanted to keep the same qualitative standards we have for the fresh produce".
The idea is to offer ready-to-eat vegetables not provided with dressing or seasoning as the customers themselves will decide how to have them: either as a side or main dish. Our biggest challenge was to achieve a product that would be similar to the one the customers would cook at home themselves, especially from an organoleptic standpoint.
Battagliola specified, "We believe that the success of these products lies in the fact that we process the produce the least possible. It is crucial to promote the quality of the vegetables. The vacuum packaging guarantees the preservability of all the aforementioned characteristics".
These products are destined to the Italian MMR. However, new opportunities could open up regarding international marketing as branded Italian products are very appreciated, and customers have been used to ready-to-eat products for many years already.
Andrea Battagliola concluded, "What is my favourite? I love them all because we processed them without taking away the incredible quality of the starting produce. However, if I must pick one, then I would say that I prefer the steam-cooked pumpkin".
La Linea Verde soc. agr. Spa
Via Artigianale, 49
Manerbio (Brescia)
Web: www.lalineaverde.it


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FRANKFORT, Ky. (AP) -- Economic damage from the coronavirus will force more than $450 million in cuts to Kentucky's General Fund.
It also could lead to a special legislative session to downsize the Road Fund. The necessity for budget cuts was officially set in motion Friday.
That's when a panel of economists reduced its revenue projections for the current fiscal year.
The job of cutting about $457 million from the General Fund will fall to Gov. Andy Beshear. He says a special legislative session is needed to reduce the Road Fund.
That's because the new projection for Road Fund revenues amounted to a larger percentage drop.


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By JEFFREY CULLEN-DEAN|Oct. 08, 2019 – 4:51 PM
Members from the Summit Family YMCA recently volunteered at the New Leaf Community Garden.
More than 40 volunteers supplied their time and labor to help complete tasks in the garden.
To celebrate 160 years of serving Atlanta-area communities, the YMCA of Metro Atlanta held two days of service with more than 2,000 volunteers at 28 different sites, according to a press release.
"As an association, we plan two days of service where we identify local nonprofits and do volunteer work to support their mission, service and whatever it is they may need," said Shane Gann, Executive Director at Summit Family YMCA.
A Summit YMCA staff member who had volunteered previously NLCG suggested the nonprofit and reached out to them to see if they needed any work done, according to Gann
"They had done work with NLCG previously and asked if they had any projects they needed help with," he said.
Gann said the volunteers raised garden beds, pulled weeds, spread mulch, replaced the roof of a shed, installed solar lights and replaced greenhouse panels.
"It was a great day," he said.


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The Order of Saint Lazarus
Sancti Lazari Ordinis Academia Internationalis
Academic Arm of
The Military & Hospitaller Order of St. Lazarus of Jerusalem
Grand Master H.E.
Don Francisco de Borbon Graf von Hardenberg
E-Library managed by the Office of the Grand Archivist & Historian of the MHOSLJ
Cartulary of the Order
12-14th centuries
15-17th centuries
18-19th centuries
20th-21st centuries
Secondary Sources
Relevant videos
Torri ta' Lanzun - Archives
Archival collection
The available Cartulary of the Order has been published by the Office of the Grand Archivist & Historian of the MHOSLJ.
These are available at
The Modern history of the Order as based on recent archival material has been published in three volumes by the Office of the Grand Archivist and Historian.
These are available at
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Sunday, December 03, 2006
Headline Writing Contest
To Enter: Click Comments and Write Your Suggested Headline. Emailed submissions will not be considered. There is no quantity or timeframe limit. Please write only one headline per comment. Entries will be accepted through Saturday, December 30, 2006. The decision of the judge will be final. A prize, valued at $20, will be awarded.
20 comments:
I got tagged and so I thought I'd tag you. Come over and see what I'm talking about but you probably already know what I'm talking about. I don't have a headline. Well, how about "Cheese?"
- Sunday, December 03, 2006 8:10:00 PM
- Mother of Invention said...
"Here's Lookin' at You, kid!"
- Sunday, December 03, 2006 8:25:00 PM
"Caught With My Shutter Open!"
- Monday, December 04, 2006 12:50:00 AM
- honkeie said...
"Caucasians from Cracker-Land document there very first encounter of the ethical kind"
- Monday, December 04, 2006 2:54:00 PM
- Mohawk Chieftain said...
"Take a friggin' picture, why don't you?"
- Monday, December 04, 2006 11:20:00 PM
They started the shooting!
blaiq was here :)
- Tuesday, December 05, 2006 5:43:00 AM
They started it first!
- Tuesday, December 05, 2006 5:44:00 AM
'They said the chances of anything coming from Mars was a million to one, but here they are, complete with cameras.'
'Blair says "Sorry"; thousands gather to document the miraculous event.'
'King Kong is real.'
- Tuesday, December 05, 2006 5:52:00 AM
"one headline per comment." Whoops...sorry. Forgive me? :-)
- Tuesday, December 05, 2006 5:55:00 AM
- bulletholes said...
"My Momma's gonna take my Kodachrome away..."
- Tuesday, December 05, 2006 11:03:00 AM
- Mother of Invention said...
Hey, Good one, Steve! I like, Caught with my shutter open, too!
- Tuesday, December 05, 2006 2:32:00 PM
- fiwa said...
Britney Spear's 2007 comeback tour, titled "Don't look at my toot-toot" draws only a handful of onlookers at a local VFW hall in Spear's hometown.
- Tuesday, December 05, 2006 3:12:00 PM
- BlueDuck said...
"The first black resident of the town was shocked at the townsfolks' reaction to his arrival."
- Wednesday, December 06, 2006 6:32:00 PM
- Annelisa said...
"Hey Kid - You're supposed to be filming his face!"
- Friday, December 08, 2006 6:10:00 AM
- Annelisa said...
"Hey, no videos! If anyone heard the real Bush speak, we'd have an invasion!"
- Friday, December 08, 2006 6:13:00 AM
- Annelisa said...
"Stop! Adults only for Bush's portrait- You want to destroy other kids'belief only Pinoccio has a nose like that?"
- Friday, December 08, 2006 6:19:00 AM
I am so not good at this and I think my mind is in the gutter because all I can think of is, " But Mom, I have never seen one this small! " ~M sorry....
- Sunday, December 10, 2006 2:15:00 PM
'' camera malfunction lets flasher get away with murder''
- Monday, December 11, 2006 6:01:00 AM
- Mother of Invention said...
Still like Janey and Steve's best!
"Feeding time at the zoo!"
- Monday, December 11, 2006 7:43:00 AM
- bulletholes said...
I may have to defer to FIWA... Brittney and her Little Booger are tramping around jus' bout everywhere one can go to "see and be seen". FIWA is a Visionary.
- Tuesday, December 12, 2006 10:22:00 AM


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Todd and Heidi participate in 11th Annual IHC Bike Ride
WJW recently celebrated and raised a glass to the many professional and personal accomplishments at our end of the year holiday party. Professionally we celebrated many grand openings, groundbreakings, new projects, and newly licensed team members. Personally, we celebrated new births, marriages, engagements and more!
Thanks to everyone who helped make 2017 a memorable year for WJW!
Related posts


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Last year, at Ulster Correctional Facility in New York, prison guard Michael Bukowski got in an altercation with then-20-year-old inmate Ramon Fabian during a prison head count, according to the New York Times and the Marshall Project.
It did not end well for Fabian.
Bukowski was conducting a morning head count at the medium-security prison when he noticed that Fabian was speaking to another inmate. Inmates are supposed to stand in place and speak low during the head count. Fabian had arrived at the facility only a few days prior to serve a one-year sentence for a drug conviction.
After the head count, guards escorted Fabian to an area out of view of security cameras, ordered him to spread his legs, and Bukowski kicked hard between his legs.
Unable to get up, he lay on the floor moaning. Shortly after, he was taken to an Albany, New York hospital, where doctors removed part of his right testicle.
In July of 2014, corrections officials determined that Bukowski had not only used excessive force but lied about it afterwards. Despite the determination, a union intervention prevented his firing.
The case was brought to an arbitrator, a procedure deemed a right by the union contract, whose decision is binding. The arbitrator upheld the conclusion that Bukowski had used excessive force and lied, but deemed the appropriate punishment to be 120 days of unpaid suspension rather than firing, seeing as this was Bukowski's first offense.
The arbitrator's decision did not satisfy corrections officials, who refused to allow Bukowski to return to his post. The union sued, but the New York State Supreme Court ruled that the 120 day suspension had not been appropriate and the judge ordered that the case be reheard. The union is appealing the court's decision.
Disciplinary action for corrections officers remains a point of contention between corrections officials and the New York State Correctional Officers & Police Benevolent Association. Of 30 attempts by corrections officials to fire guards since 2010, only 8 succeeded.
Misdemeanor assault charges filed over the same incident are still pending, though a conviction would not necessarily guarantee Bukowski's continued employment in corrections.


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In today's vlog, we discuss Obama's announcement of the new fuel economy standards for the auto industry, but more importantly the Kansas City Star's response in Tuesday's editorial. I'm sure there will be a blog post written on this subject soon.
Posts Tagged 'Vlog'
980 At Night Vlog: Kansas City Tax Day Tea Party Recap
No question for this vlog. I'm just recapping last night's events. If you were there, you know how incredible it was. If you weren't there, I'm working on a video to try and capture the atmosphere and the thousands of Americans in attendance. It was awesome.
980 At Night Vlog: "Should the military get more involved with Somali extremists?"
We talked about it Saturday night, and then on Easter Sunday Captain Phillips was rescued by the US Navy. I did this vlog last night before I heard that even more ships had been taken hostage. When do we get more involved militarily and do something about the extremists' training camps? I want to hear [...]
980 At Night Vlog: "Are we all Socialists now?"
Awhile back, Newsweek ran a cover with a picture of our President and the headline "We are all socialists now." However, recently, a leading socialist thinker wrote a column in the Washington Post that claimed President Obama is far from being a socialist, he's barely even a liberal.
We can't go a day without hearing claims [...]


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Are you in the mood for some traditional, authentic, Mexican cuisine? At Cancun Mexican Grill and Bar that is exactly what you are going to find.
Indulge in mouthwatering tacos, quesadillas, enchiladas, chalupas, tostadas, and much more. Our experienced staff is eager to help you in making a decision that suits your individual taste choices. We have something on the menu for everyone in your family.
When you have to host the next birthday party, meeting, or other social event, do it in our roomy banquet facility. We can take care of the cooking and cleaning so that you can enjoy time with your guests. You and you friends and family will love our fun atmosphere and great Mexican foods.
We can host you and your date in a small, intimate setting or your large group so that the entire family can join you. Call ahead to book your reservations to ensure you get the service you are expecting. We are locally owned and operated.
Enjoy the personal attention you and your family get when you come and dine with us. Our friendly and knowledgeable staffs are here to make your dining experience the best in the Cedar Rapids area.


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Treasures of Sicily
Our top Italy tour, a comprehensive journey, exploring Sicily's cultural heritage and ancient sites, visiting Syracuse, Piazza Armerina, Selinunte, Marsala, Monreale, Cefalù and Mount Etna
Sicily is a treasure trove of a destination when it comes to history, natural beauty and cultural curiosities. Located off the southern tip of the boot of Italy, it is the largest island in the Mediterranean Sea.
The island has its own dialect, Sicilian, which draws on Greek, Catalan, French, Arabic and Spanish, reflecting its fascinating history. Since the earliest inhabitants in 12,000 BC, Sicily has been occupied, invaded and settled dozens of times. Phoenicians, Carthaginians, Greeks, Romans, Arabs, Normans, and the Spanish have all played their part in the island's past.
Discover a slice of Sicily's past in every building. A beautiful Greco-Roman theatre in the picturesque town of Taormina. A stout but imposing 13th century castle on the far point of Ortygia Island. The ornate 7th century Cathedral of Syracuse, which has been both a mosque and a church in its long history. The Gothic Monreale Cathedral in Palermo with its gilded Byzantine frescoes. Each building reveals a new and unique aspect of Sicily's rich past.
As varied as the history is, so too are the landscapes open to visitors. Explore golden sandy beaches and wild coastlines punctuated by rocky outcrops. Venture from the craggy reaches of Mount Etna, whose crater dominates the eastern skyline, to more gentle hills, spreading along the western region.
One of the main draws of Sicily is its world-famous cuisine. Everything you taste on the island is flavoured with sunshine. Nowhere is this more true than in the use of citrus fruits in cooking across the region. From lemons and limes to navel and blood oranges, the fresh flavours pop up where you least expect them. All our tours to Sicily include plenty of time to dine out on the fresh flavours of the island and, if you choose to, sample some of the region's delicious wines.
Weather & Climate
Summers are hot and dry and temperatures more akin to those in North Africa prevail, often reaching above 30°C.
When to go
Most Italians take their holiday in July and August so prices, and crowds, can soar during these months.
The beautiful island of Sicily is one of our most popular destinations, and with good reason.
Departures up to 2020!
Welcome to our Worldwide Journeys bringing you innovative itineraries delivered by travel experts.
Our brand new Great Journeys of the World brochure, with its new look, feel and vibrancy, discerning travellers the perfect combination of luxury and service.
Our 'Secret' itineraries have been specifically designed to introduce lesser-visited places that afford a more personal but equally rewarding experience.
Our 'Wildlife' itineraries visit some of the few truly wild places left on earth, complementing our ever popular wildlife experiences.
Welcome to our new River & Coastal Cruises brochure bringing you 55 innovative cruise itineraries delivered by travel experts.
Within this compilation of travel arrangements we have sought to 'amaze and surprise' the first-time visitor and enthral the returning traveller.
We are delighted to introduce our new edition of 'Singular', an exceptional collection of Solo Group & No Single Supplement arrangements.
Our latest edition of Highlights presents a new portfolio of great value tours worldwide offering a select range of concise, more affordable, itineraries.


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Thick layers of foundation, heavy eyeliners and bold lipstick colours are things of the past. Less is more, so as speak. The no makeup-makeup look is taking the hype right now and it's here to stay. Here's our guide on how to get the natural makeup look! Step 1: Prep Your Skin It …Read More »
5 Food Habits To Reduce Your Monthly PMS Symptoms
Are you suffering from the monthly pains and discomfort that usually comes before your menstruation? Check out these tips to relieve you from PMS in a natural way. PMS or Premenstrual syndrome is common to 85% of women. The horrors of it can occur as early as 2 weeks before your menstruation. …Read More »