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Todd Pietzsch from BECU joins us live in studio. 05-17-17
Wednesday, May 17th
Wednesday, May 17th
Caller Mark refinanced his mortgage and bought a new truck, wants to know where to invest his extra cash. A texter is in debt and leasing a car, wants to know if they should declare bankruptcy. Caller Jim has a bunch of debts and wants to know if he should roll it into one payment.
Transcript - Not for consumer use. Robot overlords only. Will not be accurate.
You presented KI DSW financial advisor appeared. And Todd. I invented numbers so I know how they still afford speech. He got questions. Todd may have answers. All the. Does Todd is a fine member. Owner of BE CU and that's cool thing about that credit union men if you live work or attend school here in Washington State you can be a BEC member is well. Todd a 25 years of experience the financial services Anderson so he yeah I mean he's got access to just a lot of information also if he doesn't know it. He's against Troy towards a website that does or to tell you the PC you might have the answers for it either way. Todd is here to answer your questions at 206421. Rock rocket 7625. You can also tell estimated 77999. Mark in Seattle you around the rock right on and PGA show and big you guys rock thanks but it. I Iraq I am very important question is charged. A game art our our ear to deal IA refinance. My mortgage. Market chaos. My son's college loan. I have some money left over bought a new truck last year. I am wondering if I wanted to go ahead and add extra money we're talking about 25 K here so they are bad. Get down on it trot chew. Worrying mare a loan down to almost all year off. Or do I wanna put step back aren't marred. Moon. They're young toddlers and answer yeah well here's what here's the thing mark we don't wanna use our our house says the 18 am we did that. And you know ten years ago people got in trouble went out and went down. That being said I mean if you're disciplined year interest rate would interest rate on your loan. Which one I you're hot air house. My mortgage is 2.2. Five. But is it is a variable. No. OK that's it as a really yeah I don't. Aaron yeah I doubt it. Well I mean I guess if it was me I would go ahead and pay up the car because that. Are you able to item are you able to give enough interest you're able to itemize on their taxes when you taxes. Yes okay so if it was me I would not use the house at the him however if you're disciplined. I would go hadn't paid that I would I would personally paid on loan down and I think. An and I would take those savings and I would say that mean in look at kingdom and mortgage receiving retirement look at all the rightly what your goals are. Basic basically paid a truck costs and then Jake yeah payment but I would have import data on the mortgages and extra payment to lower my. Principal EO or that would injury time are you are you contributing to a 401K or other gas out of work British Seattle VA. Okay and and I got that problem programs are and then. Okay yeah I mean that's a good strategy. I would just be cautious of not always using the houses refinancing an easing as the eight and divide. Cars and trucks in Italy's that's right. I wasn't planning on it like my my son student always important to me in it he told me he wanted to. Assuming the last year of college. On him so it made. If you can't make something that him and I thought that was really good show or. I let him go it didn't take their responsibility RNY actually and at chaos Hasselbeck. By accident and I am yeah I actually. How did you accidentally take termer right now honest to god that is the I would love to know how that accident happened as an alleyway. A little over 50000. Dollars extra so I figure that's going to be there. Graduation he just graduated Saturday. That congratulations. And it's appreciate the call markets tight you know it is tough because I'm mark was in his fifties and that's kind of like the age where you start to see maybe you might be getting close to paying off your house if you've been doing it for that the thirty year mortgage. Yet but damn when you take out delegates everything got another loan. Is that like a love thirty years and then you hear you yet he refinance student in rolled over thirty years and painted that's tough. That's very very tough but I mean mark we really went you know have that plan was gonna look like 101520 years from now we plan. Yeah MS text message about Todd are really at 779 and license until Todd thank you for directed me towards a place to help get my credit cards paid off uncle. Awesome you are they say something mean I thought I thank Todd for I think there are no broken arm homeless as they announced today stocks ball is yes I said evil Todd. Yeah that. The basis of the tenth and yet. Don't think about it scenario that I I mean Graham is a lot of people we don't get to talk to the might be speaking kind of bad to happen if we go to Texas and I'm contemplating chapter seven bankruptcy because there about the start paying. My student loans and are you drowning in credit card debt although I never missed payments and barely make it paycheck to paycheck my credit scores on China to qualify for debt consolidation loan. So I feel like is my only option my question is I'm currently leasing my car the lease is a very serious at least another vehicle so have. And equity for them to Caesar show due to practical thing to purchase the vehicle so I'm without payment in a few years. But without a lot of things going down now while. On many layers to this layers well no evil Todd makes an. The first though we don't know I bankruptcies the solution yet right we haven't. We haven't. Dug a deep deep enough to know if where if that is chapter seven or chapter thirteen hour. Or we can do work out somehow so I would go to and FCC dot org. It's a national foundation for credit counselors. On they have pre bankruptcy counseling needn't get some advice. Look really see what your debts are what your income rising what the best solution is. These student loan I think they mentioned at student loan debt that will not be discharged in bankruptcy. And he mentioned that counselors along on tax fees that he's aware of that pleases trying to. I guess you don't get some of his loans and some of whatever finances out of the way that's an important thing that we we we don't want a gloss over that that's why you gotta be careful when you do student loans. Because I as an amazing that there's one kind of long that will never get to be discharged bankruptcies and discharge everything. Not student loans. Now. There with you forever in the end the inning and garnish your wages and acres of security in retirement as well as she should just went that long but again I would reach out get some help let's come up with a plan in and make for making the right decision. His name may be racist and mean. Yeah that's why college man I'm telling ya. Boy oh boy yeah yea you really got to be Smart about make in the college decision he got to sit down with a whole family who can pay this stuff off because that's a lot of money. And some colleges man on its ridiculous. Yet PW sometimes emotions get into it to mom dad when us and you know. And I added to the best school when India in a Ford and in the end it gating and Dan and child it's and detonate you know at a college and don't get a job that pays enough and it's it is vicious circle that CIA it's it's it's pretty horrific because ameliorated she's I mean a lot of people don't realize you're lucky to get a make fifty grand a year when you graduate college and how much have you paid in order even get their fifty granted there what what I like and I and I really and encourage people to do they can't afford the four year at a key. But you know the first two years at a community college and then transfer to a four year that it did degree from net for university that you're gonna save that money you have into action. And as tigers aren't nearly as awesome. That's true in in this town yet here is that. Through got a question from Jim over here Jim of Seattle you're on the type feature VCU go ahead Jim. More valid argument that's a bad body which got. Well I got a bunch of different that's several other group BC new second mortgage main mortgage. One credit card a couple of car payments. Just cleared it would be worthwhile to go ahead. And take all the debt and roll it into one big payment on an equity payment but I house. I can just tap the one payment would be better off are credited better to go I hadn't maintain multiple accounts and just beta moss Florida. Are you are you current on your gets him. Yeah so you're able to hear your current on everything. And yes and now how about having your house. Yeah. Or did it affect you would you write you own a full lane. I don't know and I do order another piece of property flat out. And then I've got the other house that I was built a lot. At a Baghdad anyway I'm just curious what it did do it regretted it I got edit. It is to bail out six or seven different things for the bush and combine them all the water and maintain our one credit card and then the one big. Equity payment or how payment yeah that it ball rolled into. Well if you're current on year on all year credit that's 35% that's over a third of your credit score. So here if you're looking to improve your credit score that there is just being current. Is 35% of it then the other 30% is U utilization so that's how much debt you have relatives be available credit. If you're running all these cards up then on the other might be something wee wee little one a look at. As there strategies to you know to pay those down but I don't know if you an email me Jim unhappy did you know take a look at some of the details. I don't have all the information in front of me back you know we can. Play with some numbers in some different strategies that might make make the most sense and also reduce how much interest you you'll ultimately. Yeah and Jimmy Key give me an email I anybody can as a matter fact of getting gets on the phone lines today just I go to KI SW dot com. And look for motive white banner on the right side seats not smiling face. And now that's you can email Todd Helton is Bessie candidate take all your emails and answer in the besieged him and it's Fo free. All of this part of a cool thing to be ECU does and you know credit unions do rock because the fact that you can do everything you can do to bank. Yet they're not trying to make a profit so they're not gonna do stupid stuff that shall read about in the news that'll be really just making him very depressed beat American. Just started credit union that though we have this. Ya and there's a good one called BC you know take him out and be ECU dot org tied as usual time as far as flying. Thanks BJ. The next morning and that's when nine KI guess helps you.


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"xmount allows you to convert on-the-fly between multiple input and output harddisk image types. xmount creates a virtual file system using FUSE (Filesystem in Userspace) that contains a virtual representation of the input image. The virtual representation can be in raw DD, DMG, VHD, VirtualBox's virtual disk file format or in VmWare's VMDK file format. Input images can be raw DD, EWF (Expert Witness Compression Format) or AFF (Advanced Forensic Format) files. In addition, xmount also supports virtual write access to the output files that is redirected to a cache file. This makes it possible to boot acquired harddisk images using QEMU, KVM, VirtualBox, VmWare or alike." ? ??www.pinguin.lu/xmount
Listing Details

subset: commoncrawl


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Fruit cake as wedding cake? A royal tradition! Our monarchical rulers have celebrated milestone events with a time honored tradition of including fruit cake as the main dessert. Cutting the cake had a whole new meaning once upon a time and recently played a large role in the Duke and Duchess' wedding in England. Originally made with brandy, the alcohol preserves the fruit well, making it the best option for cake prior to long standing cooling methods, (like our modern day refrigerators). To respect their relatives' cultural practices, William and Kate debuted this flavored cake on their big day, with months of preparation to get just the right tang and texture.
CCE respects the cultures of our clients and their destinations' practices, therefore, we'll work closely with a local caterer in presenting a fruit cake at our couples' upcoming Tuscany wedding. Keeping with European tradition and baking expertise, we anticipate a epic display for wedding guests!
With style,


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Tyler at the Chanel J12 launch, featured on stylemeromy.com
"Model/Designer Tyler is about to launch his label online later this year tylerockit.com. An all handmade clothing line of jeans, knits and limited edition tee's. Together with musician and friend Joel you can get a little taste of their customised style."
Style File: Tyler and Joel from StyleMeRomy on Vimeo.


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Latitude: 51.6502 / 51°39'0"N
Longitude: -0.7566 / 0°45'23"W
OS Eastings: 486119
OS Northings: 195334
OS Grid: SU861953
Mapcode National: GBR D4Y.FK1
Mapcode Global: VHDVY.TSW9
Entry Name: Hughenden Manor
Listing Date: 21 June 1955
Grade: I
Source: Historic England
Source ID: 1125785
English Heritage Legacy ID: 46568
Location: Hughenden, Wycombe, Buckinghamshire, HP14
County: Buckinghamshire
District: Wycombe
Civil Parish: Hughenden
Traditional County: Buckinghamshire
Lieutenancy Area (Ceremonial County): Buckinghamshire
Church of England Parish: Hughenden
Church of England Diocese: Oxford
4/88 Hughenden Manor
Mansion, now part used as Disraeli museum and offices. 1738 core,
extended late C18, remodelled c.1860 by E.B. Lamb for Benjamin Disraeli,
Earl of Beaconsfield. W. wing added c.1900 for Coningsby Disraeli.
Red and vitreous brick, slate roofs, diagonal brick chimney shafts with
cogged pyramidal caps. 3 storeys and cellars. First floor band course,
string courses at impost levels of first and second floor windows, the lower
string cogged; dentilled cornice; stepped parapet with dentil course and
stone coping; diagonal pinnacles with stone finials; corbelled diagonal
wall-shafts. 8-bay entrance front, the 4 centre bays recessed with single
storey arcade. Ground floor left bays and first floor have cross windows;
tall 3-light transomed windows to ground floor right; horizontal sliding
sashes to second floor, 3-light to outer bays, 2-light to centre. All
windows have narrow horizontal glazing bars and segmental brick hoods
with diagonal flanking pendants. Glazed arcade with 4-centred arches of
3 orders, dentil cornice and parapet as before. Entrance in third arch.
2-storey, 2-bay projection to right, formerly a service block, with matching
c.1900 service wing at right end. Garden front is of 9 bays with canted
projections to bays 3 and 7, stone steps to centre bays, and cellar
windows to remainder. Tall ground floor windows with large arched lower
lights and twin top lights; other fenestration similar to entrance front.
Both fronts have small stone coats-of-arms to centre. Interior: 2 mid
C18 stone panelled fireplaces with keyblocks and cornices;late C18 plaster
ceiling to N.E. top floor room, with moulded cornice and bowl-of-fruit motifs
on frieze. Ground floor rooms and staircase Gothicised by John Norris c.1840
and Disraeli c.1860: fan-vaulting and moulded 4-centred arches to hall;
former library with ribbed ceiling and screen of 2 4-centred arches;
dining room with heavily moulded Gothick alcove, traceried wall panels,
and ribbed ceiling with small wooden pendants; staircase with wooden
balustrade of ogee arches, ribbed plaster soffits and traceried skylights.
Circa 1900 doorcases and alterations to N.E. corner. Between 1848 and
1881 Hughenden was the home of Benjamin Disraeli, Prime Minister 1868 and
1874-80. Now property of the National Trust.
Listing NGR: SU8621294925
This text is from the original listing, and may not necessarily reflect the current setting of the building.
Other nearby listed buildings


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He is a Professor in the area of Organisational Behaviour & Human Resource Management. He has seventeen years of teaching experience at M.B.A. level programmes. He has taught at NITIE, Mumbai; IGNOU, New Delhi; Kanpur University; and Vikram University, Ujjain. Before joining academic career, he has worked with Bank of India as Industrial Relations Officer. He has published book on Managing Interpersonal Conflict. He has written more than twenty articles in journals and edited books, and has presented papers in more than thirty seminars.
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Advanced Windows Password Recovery v.220.127.116.11
Advanced Windows Password Recovery (or simply AWPR) is a program to recover all types of Windows passwords - logon password (when user is logged on), screensaver password, RAS and dial-up passwords, passwords to shared resources etc. The program also shows all users and groups (with their properties), allows to run any programs under other accounts, reveal passwords hidden under the asterisks, enable disabled controls, and run brute-force and dictionary attacks on Windows 9x PWL files. Finaly, it shows product ID and CD key for Windows and Microsoft Office installations.
Recover Windows passwords: PWL, asterisks etc. A program to recover all types of Windows passwords - logon password (when user is logged on), screensaver password, RAS/dial-up passwords, passwords to shared resources etc. It also runs brute-force and dictionary attacks on Windows 9x PWL files.
administrator, audit, login, logon, dialup, dial-up, vpn, secrets, credentials, recover, password, security, network, windows, user, sharedresources, screensaver
- Advanced Windows Password Recovery
- 220.127.116.11
- ElcomSoft Co. Ltd.
- Win95, Win98, WinME, WinXP, WinNT 4.x, Windows2000
- Shareware
- 1.69 Mb
- 914
- $60.00
Review Advanced Windows Password Recovery
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WARNING: Those of you who really know me will read this understanding my sense of humor, and realize how funny it would be if I were telling it out loud. Those who don't know me as well (because this blog is going to attract so many people, you know), should know that I have a certain amount of sarcasm in my humor.
So, on with the story...
Anyone who really knows me knows that I set aside 2 hours every Sunday for my Sunday nap. I've done it for so long that my body says, "OK, this is Sunday, it's nap time, I'm shutting down now." I'm a night owl, so this is my time to catch up on sleep that I've missed. So every Sunday, somewhere between morning and evening church services, you will find me on my broken couch in my living room. (The couch isn't great to sit on because it sinks in the middle, but works just right for napping - ask Jenny, she's experienced the couch effect before.)
Of course, nothing of this world is actually sacred, but my nap certainly comes close.
So today, I had settled in on the couch and just fallen asleep when my phone rings. At that point, I am not getting myself off of the couch. If I answer the phone when I'm half asleep, I like most people, am not too coherent, and I tend to be a bit grouchy. For the sake of anyone who decided to call me on Sunday afternoon, I let the machine get it.
It's a co-worker calling everyone to remind them of the office Christmas party tomorrow because it had snuck up on her. "Don't forget to bring your chips and guacamole!," she adds in a cheerful voice.
I have to admit, there's no way I was going to forget the party tomorrow (maybe I would forget the guacamole when I walked out the door, but I could come back for it) because I know it's going to mean about a 3 hour work day which is both a blessing and a curse (anyone watch Monk?). Work a couple of hours, production meeting, work a little more, go to the party, possibly back to work for a little (depends on how many steals we allow in the Chinese Christmas game), leave early for an appointment that I've been ready to go to for a week.
So, I say to the machine, "yes, the guacamole" and fall back to sleep.
Fast forward to an hour later.
That's the sound of the train about 25 yards behind my house blaring it's very loud horn as it approaches an intersection. Talk about a wake up call. I'm still not quite ready to remove myself from the couch quite yet, so I try to go back to sleep.
Then the phone rings. I think to myself, "Mother [in frustrated moments she gets called Mother], are you seriously calling me now?" Because there is already a message on the machine, the answering machine comes right on. Click.
I prepare myself because I know what comes next. The cell phone.
Yep, it starts ringing. Fine! I get up to answer the phone. Caller ID: Mom. I hear her on the other end as I flip the phone open, "She might be asleep..." You think?
"Did I wake you up?"
"Well, actually the train did, but when the phone rang, I wasn't motivated to get off the couch." (Remember, I warned that I'm grouchy when I get suddenly woken up.)
"If you're agitated, you have no reason to be. You haven't had the afternoon I've had. Pops locked the keys in the truck and we were out at the dinosaur park in Glenrose."
"Well, it's a smart thing to do to take your keys out of the car anytime you get out, even if you are just stopped for a minute."
With my obsessive-compulsive nature, I either take them out or roll the window down so that I can reach in if I do lock the door since I do it automatically. And I will point out that this is not the first time that Dad has done this. Paige was giving him grief, I'm sure since she recently had a similar experience with her dad. Anyway, for my parents and niece Paige, that ended being an all afternoon experience involving a locksmith.
And now I am fully awake. I really needed to get up at that time anyway.
So, do any of you have a "Sacred Sunday Nap"?
Welcome to the online home of Audra Jennings, a book publicist and crafter. Here I share about both. I hope you'll find books you'll want to read and crafts you will want to order. I live a rather boring, single life. At times I would like to think I am humorous. The kids I teach in Bible class tend to think so. I also blog about current seasons of The Bachelor and The Bachelorette. I don't know why, I just do.
Sunday, December 14, 2008
The Sacredness of the Sunday Nap
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It seems cliched, until you realize this was the one that started the cliches. It's also an interesting study in story-making–Clint Eastwood's Man with No Name has no motivation for interfering in the border town's power struggle, nor for helping its downtrodden citizens. Yet we don't worry about it. Maybe we accept that it's what Western heroes do and just want them to get to it.
July 7, 2011
July 3, 2011
Touch of Evil in 13 seconds
It's so refreshing to be reminded that films can be over 50 years old and still have sharp, twisty plots and incredibly rich dialogue. Touch of Evil stands up after all this time as a noir masterpiece. It's a mature movie for a mature audience (I mean that in the best possible way). It's one of those films that made me feel smarter.
The Birds in 13 seconds
Why does spoiled rich girl Melanie chase dashing Mitch to his coastal hometown? What's the attraction between these two divergent personalities? Will Mitch's mother interfere? What about Annie, his old flame? And does Melanie bring something more sinister with…wait! Who needs answers and resolution when we's got… BIRDS!!!!
July 1, 2011
Notorious in 13 seconds
I know Hitchcock was a genius, and Ingrid Bergman is quite possibly Hollywood's most beautiful woman ever. But I have a hard time suspending my contemporary sensibilities enough to believe her cheesy pillow talk with Cary Grant, and the end arrives too lazily. It's much better than the similarly-plotted Mission Impossible II, but at least it had a nice Tae-Kwon-Do match at the end…
March 7, 2011
The Seven Samuri in 13 seconds
It moves surprisingly fast for its three-hour running time. The cinematography is remarkable–instead of today's quick cuts and over-stylized sequences, we see lots of long, unedited shots. Counter-intuitively (to me, anyway), it actually ramps up the tension–you realize these characters are fighting for their lives. Their swordplay isn't awesome–they just want the other guy to die reeeeaaalll bad.


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Melbourne Victory relinquished a one-goal lead before succumbing to Green Gully 5-2 in a pulsating PlayStation 4 NPL Victoria affair on Saturday afternoon.
The opening exchanges were scrappy, with a stiff breeze making free-flowing football difficult to come by.
John Maclean had Victory's best chance early on, making a daring run to latch onto Joey Katebian's delicate pass.
But it was Gully which broke the deadlock courtesy of Liam Boland, who curled his set piece beyond the Victory wall and a helpless Lucas Spinella.
Matt Reid threatened to add a second with a glancing header that sailed over the crossbar just after the half-hour mark.
It was left to Katebian to bring Victory back on level terms. The captain converted from the spot after John Maclean was adjudged to have been held back as he approached the byline.
The joy continued just after the interval, when Reid handed Victory another favour, this time slicing the ball into his own net to hand the hosts a 2-1 lead.
It would be short-lived though, as Daniel Jones' graceful lob dipped beneath the woodwork to level proceedings in the 66th minute.
Gully struck again soon after, taking the lead through Nick Krousoratis, who slotted the ball into a vacant net.
Krousoratis added another goal just seven minutes later, increasing Gully's lead.
Evan Christodoulou added the final goal a minute into second-half injury time to confirm the result.
Victory travels to Olympic Village to face Heidelberg United in its next PS4 NPL Victoria match on Sunday, May 22.
Earlier, Victory's under-20 team ran riot once again, putting five goals past Green Gully to no reply.
Melbourne Victory 2 (Katebian 45', Reid 46' og)
Green Gully 5 (Boland 25', Jones 66', Krousoratis 77'/85', Christodoulou 90+1')
Melbourne Victory 5 (Cavallo 7'/74', Hidic 17', Stanisavljevic 52', Sette 65')
Green Gully 0


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Femen leader Oksana Shachko, 31, found dead after years of winding up Putin with topless feminist protests
The topless activist was found dead in her Paris flat on Monday
A FOUNDING member of controversial radical feminist movement Femen, Oksana Shachko, was found dead in her Paris apartment, yesterday July 23.
The 31-year-old from Ukraine became known for her topless protests with Femen which regularly challenged the policies of powerful groups and individuals.
These included President Putin, whose supporters are said to have once abducted her while he was visiting Shachko's home country, it is claimed.
She later escaped to France, where her body was found with a note at her apartment on Monday.
Inna Shevchenko, a Femen member based in the French capital, said: "It is with great regret and deep pain that I must confirm the death of Oksana."
As Paris detectives launched an enquiry, friends paid tribute to the young woman.
Friend Anna Hutsol said: "We mourn together with her relatives and friends", adding that she was waiting for "the official version from the police."
Femen member Alisa Vinogradova told news agency Central European News: "We are shocked and waiting for the autopsy results."
Before her death, Ms Shachko posted on Instagram, saying: "You are fake," but it is not clear who was the target of her post.
Her close friend Valeriy Puzik wrote on Facebook: "I have known you since you were 16 and I never could imagine that you would take your own life... You were from another planet, a comet that is flying by. I am recalling a handful of memories about you, R.I.P."
There was no immediate comment from Paris police or prosecutors about her death and a post mortem is being carried out on her body to confirm the cause of death.
Since it was founded in Kiev in 2008, the Femen movement has won notoriety in Europe for its stands - usually topless - against sex tourism, homophobia and religious institutions.
Shachko was one of four feminists who a decade ago founded Femen in Ukraine, the eastern European state involved in a bitter territorial dispute with Putin's Russia, which annexed its Crimean peninsula using military force in 2014.
Femen regularly organised topless protests against reactionary regimes, and the institutions that support them.
Now based in Paris, it claims to be an enemy of 'patriarchy in its three manifestations – sexual exploitation of women, dictatorship and religion.'
In April 2013, five members launched a "topless ambush" of Putin as he visited the Hanover trade fair with German Chancellor Angela Merkel.
Two years earlier, Shachko herself briefly disappeared during a Putin visit to Ukraine, with her colleagues saying "unknown agents" believed to be linked to Putin had beaten her up.
In 2011, she was also one of three Femen activists arrested in Belarus after staging a topless protest against President Alexander Lukashenko.
There were claims that the trio of women had their hair cut off by force, and then they were threatened with being set on fire.
Since moving to France in 2013, and being granted political refugee status, Shachko had officially ended her day-to-day work with Femen, and was trying to build up a career as an artist.
If you are affected by any of the issues raised in this article, please call the Samaritans on (free) 116123.


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THE ruling in the Samjhauta Express bombing acquits all four accused. Twelve years after the incident, the apparently strong indictment of the accused has been trumped with a simple one-line message: the investigating agency has failed to prove the conspiracy charge and the accused deserve the benefit of the doubt.
The fact that the prosecutors failed to apprehend three out of the eight people it wanted to charge-sheet is in itself indicative of just how impossible the trial was. Reports say there are no plans to challenge the ruling in a higher court.
The ruling is an insult to the 42 Pakistani victims of the Feb 18, 2007, bombing of the train that had set off for Lahore, and a sharp reminder of how opponents of subcontinental peace are allowed to get away with the biggest crimes.
It is a rebuke to the more than 20 victims of the explosion with Indian nationality and it is a snub to the efforts of those investigators of Haryana, where the incident took place, who tracked the suspects in a high-pressure probe.
There is a painful contrast between the work of police investigators assigned to look into this blatant act of terror in the initial stages and a special court of India's National Intelligence Agency which announced Wednesday's judgement.
That police team overcame all kinds of pressure to first reject the opinion that this was an act carried out by a Muslim extremist group, and then to actually announce it was a job carried out by organised Hindu extremists.
The prosecution termed it a criminal conspiracy which threatened the "unity, integrity, security and sovereignty" of India.
If these strong words offered any hope to the families of the victims and to pro-justice people generally, the rhetoric eventually gave way to the usual politics.
True to form, Delhi tried to find refuge in the exchange of allegations with Pakistan where both sides have pointed a finger at the other for not seriously prosecuting groups blamed for terrorist acts inside each other's territory — this despite many appeals that have highlighted what dangerous consequences a half-baked probe of such acts could lead to.
Pakistan's reaction to the Samjhauta ruling has been as predictable as it is sharp. Islamabad's response aptly underlines the fact that the terrorists had publicly confessed to their 'odious crimes'. It says the verdict smacks of duplicity and hypocrisy and exposes the Indian policy of patronising Hindu terrorists.
The distinction though is that it did begin with confessions — but the proceedings progressively degenerated into a cover-up for the accused.
On its part, Pakistan can do better than India by taking the Mumbai terror case to its logical conclusion. This would show the world that it is serious about combating militancy and be a befitting response to India that stands exposed after the Samjhauta ruling.
Published in Dawn, March 22nd, 2019


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Industrial High Pressure Blower
Product Code : 04
Price And Quantity
- Minimum Order Quantity
- 1 Piece
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Product Description
Fulfilling the demands of customers as a Manufacturer, Supplier, Exporter and Trader we are engaged in offering Industrial High Pressure Blower. Our offered product comprises cast-aluminum wheels, spark-resistant, and PTFE material of construction, that helps to minimize the air leakage. This blower is designed to resist high temperature conditions and has the ability to handle corrosive as well as abrasive particles. Moreover, we provide this Industrial High Pressure Blower to our customers in various specifications as well as provide customization option for the same.
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Digital Pressure Gauges with Diaphragm Seals for Homogenizing Machines MAN-SF..DRM-189
With diaphragm seal
Measuring range: 0 ... 100 ... 0 ... 1000 bar
Connection: stainless steel
Diaphragm: 23.8 mm, stainless steel
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The intelligent KOBOLD digital pressure gauges are intended for indicating, monitoring and remote transmission of pressure-dependant processes in machines and production plants. Indication occurs by means of an easily visible 4-digit green LED-display of 14 mm. The version with relays can carry up to 4 alarm contacts to be set with the keypad.(backlit LCD-display). Other interfaces are available as options.
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Mr. J and I do one thing really well in our marriage. That's sharing. When we have something we both want we divvy it up and ta-da. What's his is his, and what's mine is mine. No more questions. For dinner recently we had pizza. I had seen this fantastic recipe and wanted to make it. After all this is my similar to my favorite pizza, spinach bianco, at a local place. I don't get this pizza very often because well Mr. J does not like it.
I am not really sure what convinced me, but I was definitely convinced that if I made it at home Mr. J would change his mind and fall in love with it too. Well, when he got wind of what we were having he instant started thinking of what he could do to make it more to his preferences. So my fantastic idea for dinner when to...
and Hers
Spinach & Artichoke Pizza
1 premade Whole Wheat Pizza Crust
1 c. part-skim Ricotta cheese
5 oz. frozen spinach, thawed, drained and chopped
4–5 artichoke hearts in water, drained and chopped
1 clove garlic, minced
1/8 tsp. hot pepper flakes
2 tbsp. Parmesan cheese, grated
Preheat oven to 450° F.
In a small mixing bowl, combine part-skim Ricotta cheese, frozen spinach, artichoke hearts, garlic, and hot pepper flakes.
Place the crust on a baking sheet. Spread the cheese mixture onto the crust, leaving a 1/2" border around the pizza.
Sprinkle the top with the Parmesan cheese and bake 10 – 12 minutes until bubbly and light brown on the edges.
Mr. J had to have red sauce on his half. Then I managed to remember we had 3 cheeses to add and an onion. Finally right before we popped it in the oven, the genius that I am remembered that we had 1/2 a salami roll in fridge. Mr. J was beyond happy.
After making the pizza, my half was not nearly as good as what I expected it to be. It was no where close to the spinach bianco pizza. I found the ricotta very dry. In the end I was super jealous of Mr. J's half.


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The onset of Clostridium difficile infection (CDI) has been associated with treatment with wide-spectrum antibiotics. Antibiotic treatment alters the activity of gut commensals and may result in modified patterns of immune responses to pathogens. To study these mechanisms during CDI, we separated bacteria with high cellular RNA content (the active bacteria) and their inactive counterparts by fluorescence-activated cell sorting (FACS) of the fecal bacterial suspension. The gut dysbiosis due to the antibiotic treatment may result in modification of immune recognition of intestinal bacteria. The immune recognition patterns were assessed by FACS of bacterial fractions either coated or not with intestinal secretory immunoglobulin A (SIgA). We described the taxonomic distributions of these four bacterial fractions (active versus inactive and SIgA coated versus non-SIgA coated) by massive 16S rRNA gene amplicon sequencing and quantified the proportion of C. difficile toxin genes in the samples. The overall gut microbiome composition was more robustly influenced by antibiotics than by the C. difficile toxins. Bayesian networks revealed that the C. difficile cluster was preferentially SIgA coated during CDI. In contrast, in the CDI-negative group Fusobacterium was the characteristic genus of the SIgA-opsonized fraction. Lactobacillales and Clostridium cluster IV were mostly inactive in CDI-positive patients. In conclusion, although the proportion of C. difficile in the gut is very low, it is able to initiate infection during the gut dysbiosis caused by environmental stress (antibiotic treatment) as a consequence of decreased activity of the protective bacteria.
IMPORTANCE C. difficile is a major enteric pathogen with worldwide distribution. Its expansion is associated with broad-spectrum antibiotics which disturb the normal gut microbiome. In this study, the DNA sequencing of highly active bacteria and bacteria opsonized by intestinal secretory immunoglobulin A (SIgA) separated from the whole bacterial community by FACS elucidated how the gut dysbiosis promotes C. difficile infection (CDI). Bacterial groups with inhibitory effects on C. difficile growth, such as Lactobacillales, were mostly inactive in the CDI patients. C. difficile was typical for the bacterial fraction opsonized by SIgA in patients with CDI, while Fusobacterium was characteristic for the SIgA-opsonized fraction of the controls. The study demonstrates that sequencing of specific bacterial fractions provides additional information about dysbiotic processes in the gut. The detected patterns have been confirmed with the whole patient cohort independently of the taxonomic differences detected in the nonfractionated microbiomes.
Clostridium difficile infection (CDI) is a nosocomial disease associated with broad-spectrum antibiotics, such as clindamycin and fluoroquinolones, but cases without records of previous antibiotic treatment are also regularly reported. CDI is commonly treated with the antibiotic vancomycin or metronidazole (1, 2). At the time of CDI diagnosis in daily clinical practice, some hospitalized patients are already under preventive multiple antibiotic treatments, but some of them do not take any antibiotics at all. This means that at the time of sampling, the gut microbiome of the CDI-positive patients may be disrupted by multiple antibiotics (3, 4). This may be the reason for the results of metagenomics studies comparing CDI-positive and CDI-negative patients being very elusive (5, 6).
The 16S rRNA gene-based analysis and metagenomics also take into account dead or quiescent bacteria present in the collected fecal samples. However, for an exact explanation of dysbiosis mechanisms during CDI, the active bacterial cells must be distinguished from the dead cells. One of the possible mechanisms for opportunistic pathogen invasion is that the antibiotic treatment may alter the activity of certain commensal species, which under normal conditions inhibit the growth of pathogens. An alternative is that some members of the disturbed gut microbiome may become metabolically more active and may start to produce increasing levels of sialic acid, the primary bile acid taurocholate, and carbon sources such as mannitol, fructose, sorbitol, raffinose, and stachyose, which opportunistic pathogens may use for expansion (7, 8). The active bacteria can be distinguished from the inactive bacteria by fluorescent labeling of specific cell targets, e.g., cell wall and intracellular RNA (9–11). Cells growing under optimal conditions have fast cell division and therefore have high intracellular RNA content (12). Such cells are here referred to as "active cells." In contrast, the cells waiting for the optimal growth condition have low RNA content and are referred to as "inactive cells."
Moreover, in order to explain the infection processes connected to the dysbiosis, immune system recognition patterns must also be taken into account. The first line of defense in protecting the intestinal epithelium from pathogens is formed by intestinal secretory immunoglobulin A (SIgA), which coats 25 to 75% of all gut bacteria, including the commensal and pathogenic species (13). The SIgA opsonization of commensals and pathogens results in two different outcomes: the first being that the commensal SIgA-coated bacteria are maintained within the gut lumen, and the second being that the SIgA-coated pathogens attempting to cross the epithelial barrier are removed (14, 15). Previous studies revealed that healthy individuals share a core of SIgA-coated bacteria (11, 16). It is not known whether the taxonomic patterns of SIgA-coated bacterial fractions of CDI-positive patients and the CDI-negative control group differ. It was found that antibiotic treatment provides a "blooming" opportunity for specialized pathogens (such as Neisseria gonorrhoeae or Corynebacterium diphtheriae) which are able to avoid SIgA opsonization by covering their cell surface with molecules (e.g., sialic acids) produced by antibiotic-resistant species (17, 18). It is not known whether such a mechanism exists in CDI.
The microbial composition of the active bacterial fraction and of the fraction coated with SIgA can be determined by 16S rRNA gene sequencing of labeled bacteria selected by fluorescence-activated cell sorting (FACS) (9, 10, 11, 12, 16, 19). We hypothesized that C. difficile may in fact be a characteristic species of one of the separated bacterial fractions. The proliferation of C. difficile in the intestine may be connected to the increased activity of antibiotic-resistant species or to the eradication of sensitive bacteria. In addition, it is not known whether C. difficile is recognized by SIgA as a common pathogen or whether it is able to avoid SIgA opsonization. Our objective was to find statistically relevant associations between the microbial composition of active/inactive and SIgA-coated/non-SIgA-coated fractions and the patients' medical data.
The participants in this study were 24 hospitalized patients at the Beth Israel Deaconess Medical Center (BIDMC), Harvard Medical School, Boston, MA, USA. They have been tested by the routine Illumigene assay for CDI, as they either presented symptoms similar to CDI or fell within the CDI risk category. CDI was diagnosed in 12 of these patients, while 12 were CDI negative, as shown in Table 1.
The quantitative PCR (qPCR) assay detected specific C. difficile 16S rRNA gene sequences in 16 samples of our cohort; it formed 0.0001% to 0.881% of total gut microbiota (Table 1). Four of these patients were considered clinically CDI negative by an Illumigene assay, and the absence of toxin genes was also confirmed by a qPCR targeting C. difficile toxins A and B, indicating that these patients were colonized by nontoxigenic C. difficile strains. In six CDI-positive patients, the burdens of toxin A genes quantified by qPCR were 1.4 to 5 times lower than burdens of toxin B genes (Table 1).
The aliquots of fecal bacterial suspensions of the 24 samples were processed in two subsequent rounds of cell sorting: (i) SIgA-coated versus non-SIgA-coated bacteria and (ii) active versus inactive bacteria (Fig. 1A). The resulting fractions have been designated IgA-pos-F, IgA-neg-F, active-F, and inactive-F, respectively. The counting of the fluorescently labeled cells showed that the average proportion of cells belonging to the IgA-pos-F fraction was lower (39.67% ± 7.73%) than the average proportion of the active-F fraction (63.02% ± 5.56%) (see Fig. S1 in the supplemental material). When CDI-positive and CDI-negative groups were compared and, similarly, when antibiotic-positive and antibiotic-negative groups were compared, no significant differences in proportions of cells belonging to the IgA-pos-F fraction and the active-F fraction were detected (P > 0.01) (see Fig. S2).
Figure S1
Copyright © 2016 Džunková et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license .
Figure S2
Copyright © 2016 Džunková et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license .
The 16S rRNA gene amplicons of the separated fractions have been sequenced. The overall bacterial compositions of the 4 fractions of the 24 samples were analyzed together in the canonical correspondence analysis (Fig. 1B). The analysis detected differences between the microbial compositions of the active-F, inactive-F, IgA-pos-F, and IgA-neg-F fractions of the 24 patients (P < 0.001).
In the subsequent canonical correspondence analysis (Fig. 2), the overall bacterial composition of each of the four fractions was tested to assess the fit with patients' medical data (CDI diagnosis and type of antibiotic treatment). Three antibiotic categories have been taken into account: (i) antibiotics for treatment of CDI, (ii) antibiotics associated with the onset of CDI, and (iii) antibiotics with no reported association with CDI. Antibiotics were found to significantly shape the overall bacterial composition of the four separated fractions (P < 0.05). In contrast, the diagnosis of CDI positive or negative and the quantified amounts of toxin A and toxin B did not have a significant influence on the overall bacterial composition of the bacterial fractions. These findings indicate that the antibiotics have a greater influence on the composition of the microbiota than the C. difficile toxins. The microbiome of patients undergoing antibiotic treatment differed from those patients that did not take any antibiotics. The microbiome of the antibiotic-free group was characterized by the presence of the Lachnospiraceae family. Enterococcus was typical in patients undergoing antibiotic CDI treatment (metronidazole and vancomycin) in the four fractions. These findings demonstrate that a certain portion of cells belonging to a bacterial genus is active, while another portion is inactive (and the same is true for SIgA coating).
In the subsequent analysis, we compared the proportions of cells belonging to a single genus found in either active or inactive bacterial fractions. In order to simulate the decrease of bacterial activity in the presence of antibiotics in vitro, we performed batch culture experiments (see Fig. S3 in the supplemental material). Fecal suspensions were cultivated in the gut culture medium (20) with or without metronidazole. Samples with an 0.5-ml volume were taken every hour from the batch culture, fixed by formaldehyde, and fluorescently stained for cellular RNA. The monitoring of growth by flow cytometry showed that the bacterial culture with metronidazole grew more slowly than the culture without antibiotics. Slowed cell division in culture with metronidazole permitted us to spot two separate populations of cells with low or high intracellular RNA content (inactive-F and active-F). The 16S amplicon sequencing showed that the active-F fraction of the culture without metronidazole was characterized by an overgrowth of Escherichia/Shigella (76.2%). In contrast, the culture with metronidazole contained a major proportion of Enterococcus in the active-F fraction after 10 h (76.2%). However, a small portion of Enterococcus was also found in the inactive-F fraction (12.5%). This is in accordance with the results observed for the patients of our cohort undergoing metronidazole treatment.
Figure S3
Copyright © 2016 Džunková et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license .
The above-mentioned analysis showed that the majority of the detected bacterial genera were found in both active-F and inactive-F fractions and also in IgA-pos-F and IgA-neg-F fractions in the patient cohort, but in different proportions. When the proportion of bacterial genera in the active-F fraction was compared with that in the inactive-F fraction and, similarly, when the proportion of genera in the IgA-pos-F fraction was compared with that in the IgA-neg-F fraction, significant fold change differences (P value <0.01) were detected (Fig. 3A). The proportion of inactive cells belonging to the genera Bifidobacterium, Lactobacillus, and Nesterenkonia was usually lower than the proportion of active cells belonging to these genera. In patients without antibiotic CDI treatment (metronidazole or vancomycin), a significant number of Enterococcus cells were inactive. In contrast, in the patients taking metronidazole or vancomycin a greater part of Enterococcus cells were actually active. This is consistent with the results of the batch culture experiments.
The aim of the subsequent step was to determine whether the medical data influence the proportions of cells of a selected bacterial genus detected either in the active-F fraction or in the inactive-F fraction (and also either in the IgA-pos-F fraction or in the IgA-neg-F fraction). The statistically significant associations of the medical data (the three types of antibiotic treatment and the CDI diagnosis) with the data visualized in Fig. 3A have been established by a Bayesian network model. From the obtained network, we extracted the information on how medical data predict the patterns of bacterial activity and SIgA coating (Markov blanket dissection; see Materials and Methods). The results of this analysis are summarized in Fig. 3B; they indicated that the patterns of bacterial activity and SIgA opsonization differed among CDI-negative and CDI-positive patients. In CDI-positive patients, a greater portion of the cells belonging to the order Lactobacillales were inactive. In contrast, this was not observed in the CDI-negative cohort, who had significantly increased Nesterenkonia and Clostridium cluster IV cells in the active-F fraction. Nesterenkonia was also found significantly increased in the IgA-neg-F fraction in the CDI-negative cohort. Clostridium cluster XI (the cluster to which C. difficile belongs [21]) was also the typical bacterial group of the IgA-neg-F fraction in the CDI-negative cohort. This is in contrast to the SIgA opsonization patterns of CDI-positive patients, in which a significant majority of Clostridium cluster XI cells were found in the IgA-pos-F fraction. Fusobacterium was a typically increased genus in the IgA-pos-F fraction of the CDI-negative group.
The patients who were taking antibiotics associated with CDI onset (clindamycin or fluoroquinolones) were diagnosed as either CDI positive or CDI negative. Those patients who were CDI negative and were on antibiotic treatment associated with CDI had significantly increased proportions of active cells of the Clostridium cluster IV. The patients who did not take any antibiotics associated with the onset of CDI had in common increased activity of Rothia and decreased activity of Janthinobacterium cells.
Although C. difficile colonization in feces is detectable by qPCR or culture methods, C. difficile usually forms less than 1% of all bacterial gut genera. In addition, the healthy gut may also be colonized by nontoxigenic C. difficile (22–24), which was also observed in the present study. For this reason, C. difficile does not usually appear as a species differentiating CDI-positive and CDI-negative patients in metagenomic analyses (3, 4, 5, 25). The literature reviews showed that a general definition of the microbiome typical for CDI by common metagenomic approaches is elusive (5). Recent studies indicated that CDI onset is probably due to microbiome dysbiosis, which results in the loss of the protective species belonging to the Clostridiales order (26, 27). The fractionation of the total bacterial community by FACS performed in this study also helped to elucidate processes occurring during dysbiosis.
The results of fluorescent cell counting indicated that the proportion of active bacteria and bacteria coated with SIgA is very variable, in accordance with previous studies (9, 11, 13, 16, 19). Significantly decreased proportions of active cells in those patients undergoing antibiotic treatment were not observed, which might be a consequence of the replacement of dying bacteria with antibiotic-resistant bacteria. Multi-omics studies (metagenomics, metatranscriptomics, and metabolomics) showed that antibiotic treatment changes the taxonomic distribution of the gut microbiome but that the newly established community continues to maintain the previous metabolic functions of the gut (28), which supports our observation that the activity of the entire bacterial community continues on the same level after antibiotic treatment.
The gut microbiota modulation by C. difficile toxins has already been reported (3, 29), but this is the first study in which the influences of C. difficile toxins and antibiotics on microbiota are compared. The analysis of the overall bacterial composition of the individual separated fractions showed that the robust influence of antibiotics on the gut microbiota is even stronger than the influence of C. difficile toxins on gut microbiota.
Each bacterial genus consists of both active and inactive cells; however, their proportions depend on the antibiotic treatment. For example, the cells of the vancomycin- and metronidazole-resistant Enterococcus were mostly found in the active-F fraction of the patients treated with these antibiotics; however, a small portion of Enterococcus cells was found also in the inactive-F fraction. In contrast, in the patients with no vancomycin and metronidazole treatment, Enterococcus was mostly inactive. This is in accordance with the previous studies, which also showed that a great part of the most dominant genera of the healthy human gut is actually inactive (9, 10).
In the present study, a majority of beneficial Lactobacillales and Clostridium cluster IV cells were found dying in CDI-positive patients. This is in accordance with previous studies which demonstrated inhibitory effects of Lactobacillus on C. difficile growth (30). Clostridium cluster IV also contains species maintaining gut homeostasis (31). These findings suggest that the growth of C. difficile may be suppressed under normal conditions by these beneficial bacteria, while the decrease of their activity due to antibiotics induces a "blooming" of C. difficile. This process has already been described for other pathogens (32).
It has been previously reported that only a part of a single bacterial genus is coated with SIgA, while the other part remains uncoated, which may be a reflection of a strain-specific SIgA coating (19). The same comparison of the proportions of cells of a given genus in the IgA-pos-F and IgA-neg-F fractions has been made in this study, which helped to identify patterns of SIgA coating in CDI-positive patients. We detected that the intestinal immune system in CDI-positive patients recognizes C. difficile. These findings suggest that this species is not able to avoid SIgA opsonization, as a few other pathogens do (17, 18). In the CDI-negative cohort, it is not C. difficile but other bacteria, such as Fusobacterium, that have been typically found to be increased in SIgA-coated fractions. Despite the fact that in some studies the presence of Fusobacterium was associated with various diseases, Fusobacterium also belongs to the most prevalent genera of the gut of healthy individuals (33).
In conclusion, the excessive coating of C. difficile (Clostridium cluster XI) with intestinal SIgA and the decreased activity of the beneficial bacteria from Clostridium clade IV and the Lactobacillales order have been identified as markers of CDI. These patterns have been detected across the entire CDI-positive group by the comparison of the proportions of active/inactive cells and SIgA-opsonized/non-SIgA-opsonized cells for each bacterial genus by 16S rRNA gene sequencing of the fractionated gut microbiome, independent of the type of antibiotic treatment being given. On the other hand, the overall gut bacterial composition in CDI patients is mostly shaped by antibiotics, and the influence of antibiotics is even greater than the effect of C. difficile toxins.
Patients' medical data.The study included 24 patients hospitalized at the Beth Israel Deaconess Medical Center (BIDMC) Division of Gastroenterology (Boston, MA, USA) during the period between 29 May and 19 June 2014, with CDI symptoms or within the CDI risk category. The only exclusion criterion was a fecal sample volume below 5 ml. All 24 patients have been tested for CDI by a routine Illumigene assay (Meridian Biosciences, Cincinnati, OH; catalog no. 280050), with only 12 of them having been diagnosed as CDI positive. The other 12 patients were considered controls in this study. The study was approved by the BIDMC's institutional review board, and informed consent was obtained from all patients. The CDI diagnosis, sample collection, and initial sample processing have been carried out according to BIDMC guidelines. The samples were further processed according to FISABIO-Public Health (Valencia, Spain) guidelines.
The amounts of C. difficile A and B toxins, as well as the 16S rRNA gene of C. difficile, were quantified by qPCR (Qiagen, Venlo, Netherlands; catalog numbers BPVF00463AF, BPVF00464AF, and BBID-1506Zy-4).
Fractions of the total bacterial population.Fecal samples were processed as described previously (9, 11, 16). The fecal bacterial suspensions were divided into 4 tubes; all of these were labeled with a DNA stain (SYTO 62; Life Technologies, Carlsbad, CA; catalog no. S11344) to distinguish the bacteria from the cytometer electrical noise. The second staining was undertaken using one of the following: IgA-human or IgA-mouse (as an isotype control; Life Technologies; catalog numbers A24459 and M31001) or pyronin Y (staining RNA for active cell sorting; Sigma-Aldrich, Dorset, United Kingdom; catalog no. P9172-1G), while the fourth tube, representing the negative control, remained stained with SYTO 62 only. The aliquots of the fecal bacterial suspensions of the 24 samples were processed in two subsequent rounds of cell sorting (active-cell sorting and IgA-coated-cell sorting), as described for Fig. 1A. Each tube finally contained 540,000 FACS-separated cells (Fig. 1A). The proportion of cells in bacterial fractions was calculated using the "flowViz" package from the R statistics environment.
Sequencing of 16S rRNA gene of separated fractions.DNA from all the 96 samples (active-F, inactive-F, IgA-pos-F, and IgA-neg-F fractions for each of the 24 patients) was extracted at one time by phenol-chloroform extraction under sterile conditions. The V3 and V4 regions of 16S rRNA genes (34) were amplified and prepared for sequencing in one MiSeq Illumina run. In order to confirm the results, replicates of the 24 samples were sequenced.
A sequence quality assessment was carried out using the PRINSEQ program (35). Sequences of <200 nucleotides (nt) in length were not considered; 5′ trimming was performed by cutting out nucleotides with a mean quality of <30 in 20-bp windows. Eventual chimeric 16S amplicons were removed by the USEARCH program (36). The final data set resulted in an average of 163,519 sequences per sample.
Analysis of overall bacterial composition.Obtained sequences were taxonomically classified by the RDP classifier (bootstrap cutoff, 0.8) program from the Ribosomal Database Project up to the genus taxonomic level (37). Genera represented in fewer than 10 reads on average among all samples were not considered.
Canonical correspondence analysis was used for ordination of the 24 samples (for each fraction separately) in which the bacterial composition was tested to assess the fit with medical data using the "envfit" function from the "vegan" R package. The categorical medical data considered were CDI-positive or -negative diagnosis by the Illumigene assay and the type of antibiotic treatment (categorized into [i] antibiotic against CDI, [ii] antibiotics promoting CDI, and [iii] antibiotics with a neutral effect on CDI onset). The amounts of C. difficile toxin A and toxin B and the total doses of each of the three antibiotic types (shown in Table 1) were considered the numerical data.
Comparison of frequencies of bacterial genera in separated fractions.Fold change frequency tests between (i) active-F and inactive-F fractions and between (ii) IgA-pos-F and IgA-neg-F fractions were performed with the R package "edgeR." Bayesian networks were used to find statistically significant associations of the medical data with the statistically significant fold change increase of bacterial genera in one of the compared fraction pairs (P < 0.01, Benjamini-Hochberg correction) using the R package "bnlearn." The nodes of the network represented the preferential occurrence of bacterial genera in the four fractions, the CDI diagnosis, and the three types of antibiotic treatment (excluding any antibiotics started on the day of sample collection). Only bacterial genera that significantly increased in >5 patients in one of the compared fraction pairs were included in the network. The connecting arcs of the network represented a mutual association rather than causality. From a Bayesian network, a subset called Markov blanket can be extracted; it determines which bacteria predict the behavior of a selected node representing medical data (38). In our case, the nodes corresponding to the three types of antibiotics and CDI were dissected.
Nucleotide sequence accession number.Sequences have been deposited in the European Nucleotide Archive database under accession no. PRJEB8416 .
We thank John Tigges and Vasilis Toxavidis from the Flow Cytometry Core Facility of Beth Israel Deaconess Medical Center (BIDMC) of Harvard Medical School (Boston, MA, USA) for helping us with setting up the flow cytometry sorting. We also thank Kelsey Shields and Joshua Hansen from the Department of Gastroenterology of BIDMC for help with sample collection and Nuria Jiménez from the sequencing laboratory of FISABIO-Public Health, Valencia, Spain, for sequencing of the samples.
This work was supported by grants given to A.M. from the Spanish Ministry of Science and Competitivity (projects SAF 2012-31187, SAF2013-49788-EXP, and SAF2015-65878-R), the Carlos III Institute of Health (projects PIE14/00045 and AC15/00022), and the Generalitat Valenciana (project PrometeoII/2014/065) and cofinanced by FEDER. G.D. received a grant from Miguel Servet, Instituto de Salud Carlos III (grant CP09/00049), Spain. M.D. received a fellowship from the Spanish Ministry of Education (FPU2010) and was supported by a Boehringer Ingelheim Funds Travel Grant. J.F.V.-C. was supported by the fellowship from Gobierno del Distrito Federal (CONACYT-SECITI 2014), Mexico.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Received April 12, 2016.
- Accepted May 3, 2016.
- Copyright © 2016 Džunková et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license .


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verb (used with object)
- to convert.
- to pervert.
verb (used without object)
Verb Phrases
- to retrace one's footsteps; turn around to return.
- to cause to go no further or to return, as by not welcoming; send away.
- to fold (a blanket, sheet of paper, etc.) on itself: Turn back the page to keep the place.
- to turn over; fold down.
- to lower in intensity; lessen.
- to refuse or reject (a person, request, etc.): The Marine Corps turned him down.
- to hand in; submit: to turn in a resignation.
- to inform on or deliver up: She promptly turned him in to the police.
- to turn from one path or course into another; veer.
- Informal. to go to bed; retire: I never turn in before eleven o'clock.
- to drive a vehicle or to walk into (a street, store, etc.): We turned into the dead-end street. He turned into the saloon at the corner.
- to be changed, transformed, or converted into: He has turned into a very pleasant fellow. The caterpillar turned into a butterfly.
- to stop the flow of (water, gas, etc.), as by closing a faucet or valve.
- to extinguish (a light).
- to divert; deflect.
- to diverge or branch off, as a side road from a main road.
- to drive a vehicle or walk onto (a side road) from a main road: You turn off at 96th Street. Turn off the highway on the dirt road.
- Slang. to stop listening: You could see him turn off as the speaker droned on.
- Slang. to disaffect, alienate, or disgust.
- Chiefly British. to discharge an employee.
- to cause (water, gas, etc.) to flow, as by opening a valve.
- to switch on (a light).
- to put into operation; activate.
- to start suddenly to affect or show: She turned on the charm and won him over.
- Slang. to induce (a person) to start taking a narcotic drug.
- Slang. to take a narcotic drug.
- Slang. to arouse or excite the interest of; engage: the first lecture that really turned me on.
- Slang. to arouse sexually.
- Also turn upon. to become suddenly hostile to: The dog turned on its owner.
- to extinguish (a light).
- to produce as the result of labor: She turned out four tapestries a year.
- to drive out; dismiss; discharge: a premier turned out of office.
- to fit out; dress; equip.
- to result; issue.
- to come to be; become ultimately.
- to be found or known; prove.
- to be present at; appear.
- Informal. to get out of bed.
- Nautical. to order (a seaman or seamen) from quarters for duty.
- to cause to turn outward, as the toes.
- to move or be moved from one side to another.
- to put in reverse position; invert.
- to consider; meditate; ponder.
- to transfer; give.
- to start (an engine): He turned over the car motor.
- (of an engine) to start: The motor turned over without any trouble.
- Commerce. to purchase and then sell (goods or commodities).
- Commerce. to do business or sell goods to the amount of (a specified sum).
- Commerce. to invest or recover (capital) in some transaction or in the course of business.
- to apply to for aid; appeal to: When he was starting out as an artist he turned to his friends for loans.
- to begin to attend to or work at something: After the storm we turned to and cleaned up the debris.
- to change to: The ice turned to water.
- to fold (material, a hem, cuffs, etc.) up or over in order to alter a garment.
- to bring to the surface by digging: to turn up a shovelful of earth.
- to uncover; find.
- to intensify or increase.
- to happen; occur: Let's wait and see what turns up.
- to appear; arrive: She turned up at the last moment.
- to be recovered: I'm sure your watch will turn up eventually.
- to come to notice; be seen.
Idioms for turn
- not in the correct succession; out of proper order.
- at an unsuitable time; imprudently; indiscreetly: He spoke out of turn and destroyed the cordial atmosphere of the meeting.
Origin of turn
SYNONYMS FOR turn
OTHER WORDS FROM turnturn·a·ble, adjectivehalf-turned, adjectiveun·turn·a·ble, adjectiveun·turned, adjective
Words nearby turn
British Dictionary definitions for turn over (1 of 2)
verb (adverb)
- to sell and replenish (stock in trade)
- to transact business and so generate gross revenue of (a specified sum)
noun turnover
- the amount of business, usually expressed in terms of gross revenue, transacted during a specified period
- (as modifier)a turnover tax
- the number of workers employed by a firm in a given period to replace those who have left
- the ratio between this number and the average number of employees during the same period
British Dictionary definitions for turn over (2 of 2)
- British the difference between a market maker's bid and offer prices, representing the market maker's profit
- a transaction including both a purchase and a sale
- at the point of change
- about to go rancid
- not in the correct or agreed order of succession
- improperly, inappropriately, or inopportunely
Derived forms of turnturnable, adjective
Word Origin for turn
Idioms and Phrases with turn over (1 of 2)
Invert, bring the bottom to the top, as in We have to turn over the soil before we plant anything. [Second half of 1300s]
Shift position, as by rolling from side to side. For example, This bed is so narrow I can barely turn over. [First half of 1700s]
Rotate, cycle, as in The engine turned over but the car wouldn't start. [Early 1900s]
Think about, consider, as in She turned over the idea in her mind. [Early 1800s]
Transfer to another, surrender, as in I turned over the funds to the children. [Mid-1500s]
Do business to the extent or amount of, as in We hoped the company would turn over a million dollars the first year. [Mid-1800s]
Seem to lurch or heave convulsively, as in The plane hit an air pocket and my stomach turned over. [Second half of 1800s]
Replace or renew the constituent parts, as in Half of our staff turns over every few years. [Mid-1900s] Also see turn over a new leaf.
Idioms and Phrases with turn over (2 of 2)
In addition to the idioms beginning with turn
- turn a blind eye to
- turn a deaf ear
- turn against
- turn a hair, not
- turn around
- turn around one's finger
- turn a trick
- turn away
- turn back
- turn down
- turn for the better
- turn in
- turn in one's grave
- turn off
- turn of phrase
- turn of the century
- turn of the tide
- turn on
- turn one's back on
- turn one's hand to
- turn one's head
- turn one's stomach
- turn on one's heel
- turn on the waterworks
- turn out
- turn out all right
- turn over
- turn over a new leaf
- turn over in one's grave
- turn tail
- turn the clock back
- turn the corner
- turn the other cheek
- turn the scale
- turn the tables
- turn the tide
- turn the trick
- turn thumbs down
- turn to
- turn to good account
- turn turtle
- turn up
- turn up like a bad penny
- turn up one's nose
- turn up one's toes
- turn upside down
- turn up the heat on
- turn up trumps
also see:
- at every turn
- by turns
- every time one turns around
- good turn
- in turn
- not know where to turn
- one good turn deserves another
- out of turn
- take a turn for the better
- take turns
- to a T (turn)
- twist (turn) around one's finger
- when someone's back is turned
Also see underunturned.


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Rating: NNNNN
activist and mayoral candi-
date Tooker Gomberg was finally released from a Quebec jail Tuesday evening on $300 bail.
Gomberg, in Quebec on assignment for NOW to shoot video for the paper's Web site (look for his coverage this week at www.nowtoronto.com.), was arrested Saturday night and charged with participating in a riot and obstructing a police officer. His trial is set for May 24.
Speaking to NOW from inside the Orsainville jail Tuesday morning, Gomberg said he looked up and saw a wave of protestors running toward him. "By the time we realized what was happening and got on our bikes to try to run away, there were two or three green-outfitted riot cops that were on top of me. They put their riot sticks in my bicycle wheel and were on top of me and twisting my wrists back and wrestling me to the ground."
One independent eyewitness, Monica Harhay, has corroborated Gomberg's contention that he was quietly hanging around outside the security fence on boulevard René Lévesque when the police charged without warning and nabbed him.


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Academy™ font family
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Italic Handwriting Academy Cyrillic Bold is based on the letter-marker brush. Form it soft and smooth, hard words and lovingly displayed as the album in a dreamy character. Signs easily fall into line, which favors a great number of ligatures and options on how to break unsuccessful overlays. Font is suitable to try writing something heartfelt, and much to cause the desired result even at the absolute lack of meaning in the verse. Due to the fine machining perfectly perceived in very large point sizes. Designer suit, dealing with delicacies .
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VIDEO: Houston unites to face Harvey
The power of a natural disaster to destroy is rivaled by its potential to bring us together
The Houston metroplex, a sprawling kaleidoscope of a region nearly underwater.
Hurricane Harvey reaching winds of 130 mph, overflowing bayous, leaving thousands without power and claiming thousands of homes.
Citizens left lost, wading in the water only wondering where to go. The elderly, unable to mobilize, their fate in the hands of their neighbors.
In the midst of a natural disaster, a nation is trying to solve a problem.
The solution is unity.
Many of us who have lived through a hurricane know the paradox of a natural disaster. Its power to destroy rivaled by its potential to bring us together.
I believed that the power of unity can still be found, only if we know where to look.
In the case of Houston, a region of millions bracing down against a powerful storm, relying on the advice of a few trusted leaders, our families, our neighbors and our emergency responders that unity is found closer to home.
David Funchess is a recent graduate of Texas Southern University. The Houston Chronicle staff of 11 photojournalists has been working around the clock since Harvey hit Texas. They've traveled in boats, trucks, helicopters, bikes and on foot to get where you can't to show you how your community is impacted and is coming together.
Bookmark Gray Matters, a sprawling kaleidoscope.


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Preacher Profile: George Alvarado
Meet George Alvarado George Alvarado was falsely converted 8 times before he was genuinely saved on January 11th, 2004. Like the majority of people in western Churches today, he made many decisions to be a Christian, but none of them were a sovereign work of the Holy Spirit. After he was regenerated through repentance and faith, he soon received military orders to...Read More»
Preacher Profile: Adam Wright
Meet Adam Wright My name is Adam Wright and I live in Canton, Ohio. I work as a packer at RCA Rubber Company. I am married to my amazing wife, Tammy, and collectively we have 4 kids. Chrissy (27), Emily (22), Caleb (18), & Nick (17). I have an evangelistic outreach ministry called Made For This Ministries with my one son, Caleb, in the Canton area. We...Read More»
World Athletic Championship: Religious demographics – 6-27-17
London, England and England have had a very extensive history. See the picture of William Tyndale below who translated the Bible into English. The colonies were populated with Christians from England laying the foundation for the founding of the United States. Statistics from 2011: The largest religion in the 2011 Census for England and Wales was Christianity...Read More»
Preacher Profile: Mike Peek
Meet Mike Peek I am a slave of the Lord Jesus Christ, Son of God; He bought me at a high price. (cf. 1 Cor 7:23) In the year 2003, Darlene and I started attending a local Baptist Church because our son Daniel was participating in their youth group with a neighbor. In April, 2003, during a church-wide reading of a book called, "The Purpose Driven Life,"...Read More»
Preacher Profile: Ryan Denton
June 15, 2017 Ryan Denton Meet Ryan Denton Ryan Denton founded Christ in the Wild in 2016. Since then he has been engaged in gospel proclamation in the "wild" on a daily basis. Ryan was saved by Jesus Christ at age 22. Until then he'd been living with a woman out of wedlock. He was using whiskey and marijuana regularly. His cussing...Read More»
Preacher Profile: Kris Hudson
June 2, 2017 Kris Hudson Meet Kris Hudson Kris Hudson is an open air preacher who does street evangelism in Fargo, North Dakota. He has evangelized outside of Newman Outdoor Field (minor league baseball stadium), North Dakota State University (NDSU) football games, various kinds of parades, stoplights, street fairs, and on the NDSU campus. Kris grew up in a...Read More»


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Also found in: Encyclopedia, Wikipedia.
bimorph(ˈbaɪmɔːf) or
bimorph cell
(Electronics) electronics an assembly of two piezoelectric crystals cemented together so that an applied voltage causes one to expand and the other to contract, converting electrical signals into mechanical energy. Conversely, bending can generate a voltage: used in loudspeakers, gramophone pick-ups, etc


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The Slingshot UK Network
Tap into your local Slingshot network today!
Dealers - Stockists of Slingshot products nationwide.
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text:
Rescue mask
Indispensable for all rescue operators, this mask avoid any contacts between mouths during emergency reanimations. Made of transparent material, it is composed by a soft cushion and a valve with non-return membrane. It is all contained in a handy, plastic, shockproof case. Color of case may change.
Request for quote


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Publications and Research
Document Type
Publication Date
The article focuses on a collaborative project aimed at investigating the effect of integrating four classroom assignments from different disciplines with critical thinking skills. Entitled "Collaboration and Information: Critical Thinking Skills Across Curriculum," the different goals of the project include having the students understand what information is, the type of information needed and its appropriateness, and the application of research skills using critical thinking. In this study, it has been demonstrated that the greater the breadth and depth of responses to the scenario, the greater the research process skills.
Recommended Citation
Beck, Sheila and McKay, Devin, "Critical collaborations: An information literacy across the curriculum project" (2011). CUNY Academic Works.


text: Press Features For all press or partnership inquiries please contact: Kristi Finigan, 571.999.4363. 〉Local Female Entrepreneurs Launch Collaborative Covid-19 Response Initiative for #GivingTuesday 〉Local Artist Launches 'Kisses for a Cause' Charity 〉TV Appearance on Access Daily • Los Angeles 〉Performance at Sephora, Universal City Walk • Los Angeles, CA 〉News from around our 50 States: Florida 〉Michael And Sara Create Kissable Art with Lipstick Lex 〉Lipstick Lex shares the love in her art 〉Pucker Up! This Lipstick Artist Creates Portraits With Her Kisses 〉Artist Makes Paintings With Kisses 〉Kissing Artist Masters the Art of Kissing 〉Artist Alexis Fraser, a.k.a. Lipstick Lex Shows How Makeup Can Become Art See More Press Features 〉Sarasota artist paints with lipstick and kisses 〉Proof Lipstick Kisses Can Make a Serious Work of Art 〉Meet Lipstick Lex… Her Art Starts With A Kiss On Canvas (Literally) 〉Be Expert: Lipstick Lex 〉I Use My Lips And Lipsticks To Create These Paintings 〉Lipstick Artist Creates Beauty with Every Kiss 〉ArtResin Featured Artist – Lipstick Lex! 〉Artist Uses Intimate Technique To Create Gorgeous Paintings Of Nature: Kissing The Canvas 〉Sun-Kissed Summer @Harbour City 〉Sealed With a Kiss 〉Lipstick Lex 〉Alexis Fraser, The Girl Who Paints Kissing The Canvas 〉HK's first ever Lipstick Tasting Bar at Harbour City 〉Lipstick art exhibition by Lipstick Lex and Lipstick Tasting Bar at Harbour City 〉This Artist Uses Lipstick and Thousands of Kisses to Create Stunning Portraits 〉Artist Lex Kisses a Picture Full of Love 〉Harbour City's New "Kiss Art" Exhibition 〉Alexis Fraser's Solo Exhibition "Sun-kissed Summer" Held in Hong Kong 〉Contemporary Artist Uses Lipstick & Kisses the Canvas to Create Stunning Works of Art


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Community post
Ttc baby #1
Hi guys ttc #1,,31 yrs old..suffering from pcos,,,very long irregular cycles..currently cd59 no af,,no symptoms,,got a faint + on topcare bluedye test,,both digitals cvs and first response came out negative,,im 20dpo dont know what to believe..idk if just imaging the lines here but i think its pretty positive? The plus appeared like in 2mins..cant be evap..broke the test in half the line was still there..can anyone help me here? Would appreciate it!
8 Replies • 1 year ago
Pregnancy test calculator
Use this calculator to help you decide when to take your first home pregnancy test.
Replies (sorted by laughs)
As much as I don't like this brand of tests because they have so many evaps, this one does appear to have a faint positive... you would expect in most cases by 20DPO your test line would be quite dark.
I would perhaps make an appointment with your doctor to get a blood draw.
Best of luck and I hope it's a good sign for you!
1 year ago
Early pregnancy symptoms by day past ovulation
What signs and symptoms are most common on each day past ovulation?
More Discussion posts


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Should YOU Invest In Test Prep – Total College Planning
The SAT. The ACT. They are the two standardized test options currently available to students wishing to apply to the great majority of the four-year colleges and universities in the United States. These exams spur trepidation in parents and students alike – for good reason. The exams themselves generally account for 20% of the admission decision at the average U.S. institution of higher learning, and the additional peer pressure shared between students only ramps up the value we place on these rather ridiculous statistical measurements.
We all know, or at least we believe we understand, the tests and their inherent value. We associate them with entry into the college or university of our child's dreams. Make no mistake – these tests are lukewarm measurements of your child's academic abilities or their future earning potential and are simply one more tool for college admission officers to separate very good students from great students. The tests represent one more opportunity for admission offices around the country to make a slight differentiation between high GPA's held by students from Seattle, Boston, Southern California, or anywhere else around the country, or for that matter, the globe.
More importantly, we forget about the other major value standardized tests provide our families – they are highly influential in determining which students are awarded merit based aid – the free cash given by schools or outside organizations that is not determined by your ability to pay the tuition, fees, room and board, etc. I will always tell a family that the fear they feel about college is misguided – they should have some fear – but not necessarily about getting into a "good" college – that is a misnomer – there are lots of good colleges all across the country; the fear should be about how the heck to pay for it all!
So let's get down to brass tax: should you invest in test preparation for your child? I did not misuse the term. This truly is an investment that if treated properly and quality preparation is provided, can make a significant difference in your child's chances of getting into their dream school or help them make the cost of other colleges on their list much more affordable. There are many examples of students who used the power of test preparation as a tool that saved themselves and their parent(s) tens of thousands of dollars off of the college 'sticker price' that they elected to attend.
So many families go about this process the wrong way. Even some college and guidance counselors make the wrong call. I have never understood the advice that students should just go "take the test cold and see how it goes," then decide what, if anything, needs to be fixed. I think the method is right on – what I think is problematic is the notion that students should start by taking the real test cold – without any prior prep. That makes no sense. Would you think it wise to not study for an important school test, go take it without any prep, get a bad grade, then negotiate with the teacher to retake the exam, knowing you have lowered your confidence level and raised the stress over the value of the second attempt? It's just silly.
The best advice I can give is to recommend that you get your student in front of a realistic mock SAT and ACT test, before their testing window opens (generally sometime in 11th grade), to see what their natural strengths and weaknesses are, determine what remediation, if any, they might need, and help determine which test is a better fit. Over 99% of colleges today accept either test equally, so you really have options. Once you have a starting experience and some realistic starting scores, and you match those scores up with the dream schools average scores for admitted students as well as the rest of your students college list, you will know if test prep makes sense or not. Then you can look at all of your prep options and find the best match for your student, whether that be buying a book, online prep, classroom prep, or 1-on-1 tutoring.
I encourage you to contact me at your leisure with questions or concerns you may have about the testing process, the prep options, or if you would like to get in front of some mock tests. It is a free service The Princeton Review is glad to provide to your family.
Rob Schwartz
Total College Planning
5521 Reseda Boulevard,
Suite 107Tarzana, CA 91356
Steve Armanino