text:
June 23 2016
Recently we published an article titled, Conversion is Key for This Open House Expert, which detailed a story from San Francisco-based agent Nicole Nicolay. Nicole uses open houses as an opportunity to convert market watchers, tire kickers, and fence sitters into clients. In that article, she shared her proven technique for collecting contact information at open houses. Nicole explained that she quickly learned that many people write like doctors when you ask them to write their email addresses. Her fix was to digitally capture contact information by way of a landing page off her website.
In response, we had a number of agents ask for a follow up post showing how they could create a similar system. Here's how:
A landing page is a single page located on your website that highlights a single topic. Many of the most successful landing pages lead with a bold, eye-catching image, laser-focused headline and clear call-to-action. To enhance your credibility, consider including social proof or testimonials confirming the value of your offer.
Your landing page will need a form to capture the prospect's request and contact information. There are a variety of form creation tools available on the market, and many have free, low volume options. Here are a few of our favorites:
The leading WordPress plugin for creating forms, and the system utilized by Nicole Nicolay, Gravity Forms builds your lead capture system by simply dragging fields into place. Use the conditional logic to show or hide fields, sections, pages or even the submit button based on user selections. Gravity Forms comes with a onetime cost of $39.00.
2. Ninja Forms
If the Gravity Forms price tag of $39 is too much for you, try Ninja Forms. This popular WordPress plugin comes with a simple drag and drop interface and is yet another easy way to build a form for your WordPress site.
3. Google Forms
A free forms solution offered by Google that makes creating and analyzing form results easy directly from your web browser—no additional software is required. Simply drag and drop your fields into place.
4. Wufoo
Another simple solution for creating embeddable forms, Wufoo offers both a free and paid version. The free version allows users to create up to three forms with fewer than 100 monthly entries. The tool includes an easy to use form designer. Paid plans, which start at $14.94 a month, include more sophisticated features and allow for more entries.
5. Form Tools
For the techy agents out there, Form Tools could be a great option for creating single or multi-page forms. This form system is free and open source, running on PHP.
6. Unbounce
A powerful drag and drop landing page builder that will help you create mobile responsive pages without any help from a technical team. Unbounce comes with hundreds of templates to make getting started quick and easy. Unbounce does not offer a free option; their base package starts at $49.00 a month.
Now that we have reviewed a variety of forms options for your site, what information should you collect? It's important to keep the form as simple as possible; asking only for the information needed to complete the request. For example, if your goal is to have Open House visitors sign up before they leave, you may want to ask for first and last name, email address and phone number.
Consider also asking for which RPR reports they would like you to send them. For example, the Property Report, Market Activity Report, or Neighborhood Report. Also include a simple checkbox asking prospects if they would like to opt-in to your email communications. If you have a system for sending new homes by email, consider adding that as an option, too.
To view the original article, visit the RPR blog.


text: As you speak your mind, seek rather like speaking kind. When you fail anybody else, seems like you failed yourself. Don't hear the gentle voice of lie inside you. Don't. They say you cannot beat the demons by beating up on yourself. They say demons die by love. They say fall more in love with… Continue reading Dear Me: Free Yourself from Something Else

subset: commoncrawl


text:
Ed Comeau, Vision 20/20 Communications Director
Free online courses launched focusing on Community Risk Reduction and fire prevention program evaluation measures
Courses developed by Vision 20/20 in partnership with IFSTA provide valuable information to nation's fire service
Warrenton, VA (DATE) - In partnership with the International Fire Service Training Association (IFSTA) (r1.ifsta.org), Vision 20/20 (www.strategicfire.org) has developed a free, five-part training program focusing on Community Risk Reduction (CRR) as a way to assist fire departments in developing safer communities. The course presents the basic elements necessary to prepare a station-based CRR plan. It provides knowledge and motivation for implementing community risk reduction practices, and serves as an easy to access resource for fire departments across the country.
The course covers the process of identifying fire and life safety risks in communities and developing coordinated strategies between operations and prevention that improve public and fire fighter safety.
Vision 20/20 and IFSTA have also partnered to host a series of free online learning modules to improve program evaluation measurements for fire prevention programs, including public education, plan review, code enforcement, and investigations. These same evaluation tools also apply to other injury prevention and mitigation programs.
The course familiarizes students with four evaluation measures:
• Formative evaluation is associated with problem identification and the early program development stage of a prevention planning process.
• Process evaluation is related to program planning and implementation stages.
• Impact evaluation measures educational gain or risk reducing behaviors.
• Outcome evaluation methods are related to changes or reductions in deaths, injuries or property loss.
"Both of these courses provide much-needed resources for the nation's fire departments," said Vision 20/20 Project Manager Jim Crawford. "By partnering with IFSTA, we are able to make them widely available to help departments implement effective CRR programs in their communities. We appreciate the support from the Assistance to Fire Fighters Grant program in underwriting this project".
Mike Wieder, IFSTA Executive Director, said, "IFSTA is excited to host these courses so the fire service and prevention advocates can access this excellent training at no cost to the individual or to their organization. ResourceOne is the IFSTA learning management system and we are very pleased to host these two courses in partnership with Vision 20/20."
More information on both of these free, online courses is available at Vision 20/20 www.strategicfire.org and IFSTA ResourceOne r1.ifsta.org.
Vision 20/20 is an organization focusing on addressing the gaps in fire prevention, developing tools and resources, and fostering an exchange of ideas. More information can be found at www.strategicfire.org. IFSTA is the leading publisher of training materials for the fire service and supports community risk reduction with 9 CRR titles. IFSTA has a full line of print and electronic products for students and instructors including eBooks; Apps; and the IFSTA online LMS ResourceOne. r1.ifsta.org
IFE Vision 20/20 Project | 35 Horner St., Warrenton, VA 20186 | (540) 347-7488 www.strategicfire.org | Facebook www.facebook.com/strategicfire | Twitter @strategicfire


text:
Dr Iain Robert Smith is Lecturer in Film Studies at King's College London.
To celebrate the upcoming anniversary of the Apollo 11 Moon landing, Dr. Iain Robert Smith (King's College London) has compiled a list of 11 Moon-landing films ranging from relatively realistic depictions of the US and Soviet space programmes through to more fantastical tales that feature nudist camps on the Moon, Bollywood musical numbers, and lunar landscapes made of cheese.
Le Voyage dans la Lune / A Trip to the Moon (Georges Méliès 1902) (France)
Widely regarded as the very first example of science fiction cinema, Georges Méliès's Le Voyage dans la Lune depicts a team of astronomers who fly to the moon in a capsule fired by a giant cannon. The moment when the lunar capsule lands in the eye of the 'Man in the Moon' is one of the most iconic images in film history and has been referenced in everything from the TV series The Mighty Boosh through to the Smashing Pumpkins music video 'Tonight, Tonight'. Shaped by the ideas of Jules Verne and H.G. Wells, Méliès's fantastical vision of space travel was itself to have a profound influence on many subsequent filmmakers who were inspired to focus less on the scientific logistics of space travel than on the dream-like notion of taking a voyage to the Moon.
Frau im Mond / Woman in the Moon (Fritz Lang 1929) (Germany)
Two years after Fritz Lang directed the expressionist science fiction classic Metropolis (1927), he returned to the genre with Frau im Mond, adapting a novel written by his then-wife Thea von Harbou. While the film contains some elements that are less believable from a present-day perspective – such as the far side of the Moon having a breathable atmosphere – the film includes many ideas that would later be adopted in real spaceflight, such as the use of multi-stage rockets and the reverse 'countdown to zero' before a launch. This is perhaps unsurprising given that Lang enlisted one of the founding fathers of rocketry, Hermann Oberth, as an advisor on the film. In fact, the film was perhaps a little too realistic for its own good, as it was banned by the Nazis in 1937 after they feared it was too similar to their own clandestine V-2 rocket programme.
Kosmicheskiy reys / Cosmic Voyage (Vasili Zhuravlov, 1936) (Soviet Union)
The Eastern-Bloc produced a number of science fiction masterpieces including Yakov Protazanov's Aelita: Queen of Mars (1924), Jindřich Polák's Ikarie XB-1 (1963) and Andrei Tarkovsky's Solaris (1972). Despite the various successes of the Soviet space programme from the 1930s onwards, however, it is notable that relatively few of these films depict journeys to the Moon. One exception is Vasili Zhuravlov's Kosmicheskiy reys which imagines the Soviet Union preparing the first manned spaceflight to the Moon in the year 1946, a decade after the film was made. Partly inspired by the realism of Frau im Mond, director Zhuravlov hired the world-renowned scientist Konstantin Tsiolkovsky to be a technical advisor on the film, and the film attempts to depict a credible vision of spaceflight including remarkably innovative scenes of weightlessness. Unfortunately, despite a brief cinematic release in 1936, the film was removed from circulation by Soviet censors who felt it did not adhere to the principles of socialist realism and it wasn't until 1983 that the film was rediscovered.
Nude on the Moon (Doris Wishman & Raymond Phelan, 1961) (US)
One of the more bizarre sub-genres of space travel films was a 1950s-60s cycle of US exploitation movies in which astronauts discover all-female matriarchal civilisations living on other planets. With titles like Cat Women of the Moon (1953), Fire Maidens from Outer Space (1956) and Voyage to the Planet of Prehistoric Women (1968), these films would often depict the astronauts as being the only men on a planet populated entirely by women. Perhaps the most notorious was Nude on the Moon, co-directed by the prolific sexploitation filmmaker Doris Wishman, which was a fairly typical variation on the popular 'nudist camp' films of the era – except that this one takes place in a nudist camp on the Moon. Much of the plot is a thinly-veiled excuse to allow the two protagonists to take photos of the topless 'extra-terrestrials' playing volleyball or lying in the sunshine on a surprisingly tropical lunar landscape (actually Coral Castle in Florida).
Chand Par Chadayee / Trip to Moon (T. P. Sundaram, 1967) (India)
While the 2003 blockbuster Koi.. Mil Gaya (I Found Someone) was promoted as the first ever Hindi science fiction film, there were actually a significant number of Hindi science fiction films produced in the 1960s – ranging from the superhero film Return of Mr. Superman (1960) through to the Martian invasion narrative Wahan Ke Log (1967). These Bombay B-movies would often borrow and adapt established science-fiction tropes such as flying saucers or giant monsters, and it is Chand Par Chadayee that was to be the film that introduced the 'trip to the Moon' genre to Indian cinema. Starring Dara Singh, who was both a B-movie action star and a professional wrestler, the film follows his character Anand as he journeys to the Moon and does battle with the various monsters he encounters. While the film features many of the expected sci-fi elements such as robots, jetpacks and rocketships, Chand Par Chadayee also integrates numerous local elements such as the elaborate musical sequences that are characteristic of the popular Indian cinema of the period.
2001: A Space Odyssey (Stanley Kubrick, 1968) (UK/US)
Designed to shift science fiction away from the B-movie space-opera aesthetic characterised in the US by TV series like Buck Rogers (1950-51) and Flash Gordon (1954-55), 2001: A Space Odyssey was a slow-paced and deliberately enigmatic collaboration between director Stanley Kubrick and author Arthur C. Clarke. Despite Kubrick's notoriety within various moon landing conspiracy theories, 2001 actually contains relatively little material related to the Moon. Instead, the film focuses on the increasingly strained relationship between the human crew and the artificial-intelligence HAL during a lengthy mission to Jupiter. Nevertheless, one of the key sequences in 2001 is when the astronauts visit the Moon and discover a mysterious black monolith that had been buried there four million years earlier. These sequences were sufficiently convincing that they inspired the conspiracy theory that the television footage from the Apollo 11 Moon landing was actually faked by the CIA with the help of Kubrick. Indeed, these conspiracy theories have become so pervasive that they have reappeared in various subsequent films including the mockumentary Dark Side of the Moon (2002), the action-comedy Moonwalkers (2015) and the found-footage horror film Operation Avalanche (2016).
The Right Stuff (Philip Kaufman, 1983) (US)
Adapted from a novel by journalist Tom Wolfe, The Right Stuff focuses on the true story of the 'Mercury Seven' – the seven pilots who were chosen to be astronauts in the first US manned spaceflight programme that ran from 1958 to 1963. Widely acclaimed as one of the greatest films about the space race, and indeed one of the best films of the 1980s, The Right Stuff is renowned for the accuracy and authenticity of its scenes at Edwards Air Force Base and Cape Canaveral Air Force Station. The emphasis upon the personal ambitions that drove these astronauts, and the tensions this caused with their family relationships, would influence subsequent historically-grounded depictions of the space race in films like Apollo 13 (1995) and First Man (2018).
A Grand Day Out (Nick Park, 1989) (UK)
In addition to the various live-action films featuring trips to the Moon, there have also been numerous animated shorts such as the Tom and Jerry cartoon O-Solar Meow (1967) in which Tom uses a cannon to shoot Jerry to the Moon only to discover that his rodent-rival is perfectly happy as the moon is made of delicious cheese. A similar cheese-related journey takes place in Nick Park's stop-motion animation A Grand Day Out in which the eccentric inventor Wallace and his dog Gromit spend their Bank Holiday building a rocket to visit the moon in order to sample its cheese. On tasting a chunk of the Moon, Wallace declares that despite some similarities with Wensleydale and Stilton, it is ultimately "like no cheese I've ever tasted." The first of the Wallace and Gromit series, A Grand Day Out took animator Nick Park six years to make and returned the genre to its fantastical Méliès-inspired beginnings, albeit with a distinctly British sense of humour.
Apollo 13 (Ron Howard, 1995) (US)
Apollo 13 is one of the most popular 'Moon-landing' films despite the fact that, or perhaps because, it tells the story of a failed attempt to land on the Moon. Based on the account of the Apollo 13 mission written by NASA astronaut James A. Lovell, the film focuses on how the team of Lovell, John Swigert and Fred Haise managed to navigate back to Earth after an oxygen tank explosion caused them to abort their planned lunar landing. Lovell is played in the film by Tom Hanks, an actor famously meticulous about technical accuracy – co-star Kevin Bacon wryly observed that "Tom Hanks wouldn't push a button unless it was the right one to push" – and the film follows the precedent set by The Right Stuff in attempting to recreate the space race with a certain level of realism and authenticity. Director Ron Howard even managed to obtain permission to film the zero-gravity sequences using NASA's KC-135 training aircraft, best known by its nickname the 'vomit comet', and the sequences are still hailed today as amongst the most convincing depictions of space flight ever filmed.
Moon (Duncan Jones, 2009) (UK)
Harking back to the grounded naturalism of 1970s science fiction films like Silent Running (1972) and Alien (1979), Moon inverts much of the romanticism of 'voyage to the Moon' narratives by instead highlighting the bleak emptiness of the lunar surface. This sense of desolation is then reflected in the central character of Sam Bell (played by Sam Rockwell) who is all alone on a mining facility on the far side of the Moon, with only the artificial-intelligence GERTY for company. The aesthetic of the film is inspired by NASA's photos from the Apollo missions, which were themselves notorious for requiring a new type of black ink named 'Luna Nero' to be developed in order to capture the density of the blackness of space. Moon was the feature film debut of Duncan Jones; whose father David Bowie had his own close associations with Moon-landings – famously releasing the song 'Space Oddity' the very same week as the launch of the Apollo 11 mission to the Moon.
First Man (Damien Chazelle, 2018) (US)
Moon-landing films can be roughly divided into two filmic traditions. There are the fantastical, magical films exemplified by Georges Méliès's Le Voyage dans la Lune that depict the journey to the Moon as something akin to a surreal dream. On the other hand, there are films like Damien Chazelle's First Man that instead attempt to capture the stark reality of the Apollo missions and the very human fragilities of the people involved. Anchored by remarkably restrained and understated performances from Ryan Gosling as Neil Armstrong and Claire Foy as his wife Janet, the film emphasises the dedication of the NASA astronauts and the strains that this can place on their family lives. Lacking the sentimentality of Apollo 13, or the epic scale of The Right Stuff, First Man is instead a remarkably unshowy film that functions to de-mythologise the very idea of the 'first man on the Moon.'
Dr Iain Robert Smith
Dr Iain Robert Smith is Lecturer in Film Studies at King's College London. He is author of The Hollywood Meme: Transnational Adaptations in World Cinema (EUP, 2016) and co-editor of the collections Transnational Film Remakes (with Constantine Verevis, EUP, 2017) and Media Across Borders (with Andrea Esser and Miguel Bernal-Merino, Routledge, 2016). He is an AHRC/BBC New Generation Thinker.


text:
Lim Juan has posted a review of the Exogal Comet DAC ($2500) at audiofi.net. His conclusions:
"A very good DAC then, from experienced industry veterans who know what they are doing. The Comet's proprietary processing platform allows the manufacturer a measure of future proofing of the unit to protect their customers' investment which other manufacturers cannot claim to equally provide."
You can read the full review here.
You can read my review of the Exogal Comet DAC here.
You can find links to more reviews of the Exogal Comet DAC here.


text:
The Nature of Freedom
Freedom is an ancient concept that has been at the foundation of many of humanity's struggles. Slaves from the beginning of civilization understand when they do not have freedom. People living in countries oppressed by political leaders know their lack of political freedom. Philosophers ponder the nature of freedom until the concept appears clear to them. However, what is the nature of freedom? Is "freedom" an individual's ability to think or speak as he or she pleases? Does freedom come with having the financial ability to spend and invest without restriction? Or is it freedom from restraint, with the opportunity to move about the world as the individual chooses? Who has the right to freedom? Leaders or the people who follow them? Or do any of them deserve freedom at all?
We Will Write a Custom Essay Specifically
For You For Only $13.90/page!
order now
Plato and Socrates had similar points of view on the nature of freedom. This relationship is inevitable. Not only did Socrates' have an influence on Plato, ostensibly as his teacher, but also, what we know about Socrates comes from Plato's writings. For that reason, it is difficult to know how the philosophy of one may have differed from the other. However, they also differ significantly on the value of freedom and whether or not an ordinary person needed it or had the capacity to understand or value it if it were granted.
Socrates presented a bit of a dilemma for the government. He lived in a democratic society, although the idea of democracy differed in ancient Greece from the democracy we have today. Socrates, however, encouraged all people, not just citizens, to think about things that may have had an effect on their satisfaction with their roles. Many of the people who lived in that society also considered his ideas as dangerous, given that they adhered to the Sophist position that knowledge was a commodity that could, and perhaps should, be purchased rather than allowing every person in society the freedom to have access to it, regardless of position.
One thing that Socrates spoke about was the nature of freedom. Although Socrates appeared to think that the concept of freedom was manifold, he did have some clear ideas on what it was not. For example, freedom does not come from the government itself, because, the only thing that those who govern can teach about is governance. However, the Athenians seemed to understand freedom as being political in nature. The character of Socrates, in Plato's The Republic speaks at great length about tyranny. Given that the Greek republic began in an attempt to protect their citizens from invasion, this focus is unsurprising; however, it is interesting that it retains such a significance in Plato's writing. Clearly, the state has control over whether its citizens have freedom from tyranny; however, it does not dispense personal freedom to individuals.
In Book VIII of The Republic, however, Plato's character of Socrates argue that citizens in a democracy have freedoms that extend beyond that of freedom from tyranny. Citizens also have the freedom to "say and do what he likes" and to "order for himself his own life as he pleases" (216). Although the government does not overtly provide these things to its citizens and although it does not teach these things to its citizens, a good government permits its citizens to make these pursuits. However, as the Allegory of the Cave illustrates, a person is only truly free if he is able to see the truth through reason; otherwise he is not capable of understanding the true meaning of the shadows and he is unable to impart this knowledge to others (Plato).
In Socrates' philosophy, the truth was difficult for even a philosopher to find, as evidenced in many of his arguments, although best supported by "proof" in the Allegory of the Cave (Plato). This difficulty resulted in his defining freedom as ephemeral, rather than giving it distinct characteristics. Plato, however, felt that truth could be defined in a concrete fashion. Plato, like Socrates, also disagreed with the Sophists who suggested that "freedom" was a subjective and something of human invention. For Plato, however, freedom was an actual privilege, which. Citizens could only have a harmonious life, according to Plato, only if they gave up some or all of their individual freedoms, which were often regulated by a person's position in society. Guardians, for example, had no personal freedoms at all. They were expected to live a communal life because they had absolute power; however, given their ability to reason through "only by the criterion of knowing and not knowing" Socrates (through Plato) seemed to think them worthy of this power despite their relative lack of freedom.
The people who lived under the rule of the Guardians also had no freedoms; Plato felt that the people were ignorant and corrupt and so could not be trusted to make correct decisions. Unlike Socrates, then, freedoms were reserved for only a select few in society, who he deemed to be wise enough and fit enough to use them without harm to others. Not only is it possible for only these select few to understand wisdom, this class also determines which community freedoms the citizens and other people within the state would have. In addition, the state limits the individual's access to knowledge, which Socrates considered essential to freedom..
Using Plato's reasoning, however, an ordinary person cannot rise from his position. Those who rule in a democracy, according to Plato, can only rule wisely if they also govern the wise because the two are closely entwined. Despite the state containing both freeman and masters, such a political state is completely enslaved, in that Plato views even the best of the people to be "miserably degraded and enslaved" (236)
Both Socrates and Plato viewed the concept of freedom as being related to the state. In Socrates' view, a democratic state allowed people to pursue knowledge, which in turn created the ability for those people to greater understand the true meaning of the world and the true meaning of freedom. In Plato's view, personal freedom was limited in a non-democratic state, with freedom being defined in the more traditional Athenian view as freedom from tyranny. In both cases, the average person did not understand the meaning of freedom; however, in Plato's view of the world, neither did the average person deserve freedom.
Work Cited
Plato. The Republic. Mineola, New York: Dover-Thrift, 2000.


text:
You've herd about Urban Cow! You've seen the bumper stickers and wondered, "What race is that?" While training on the roads and trails, you've spied the cool cow shirts and said, "I want one!" And you know you want that cowbell medal. It's legen-dairy!
It's time to quit talking to yourself and register for the Urban Cow Half Marathon, Two-Person Relay or 5K and see what all the fun is about.
Join us for the 15th Annual Urban Cow Half Marathon, Two-Person Relay and 5K Run/Walk on Sunday, October 6, 2019 in William Land Park, Sacramento, California.
Registration fees
Half Marathon ClosedOnline registration closed
$68.00Jan 1 – May 31
$73.00Jun 1 – Aug 31
$78.00Sep 1 – Sep 29
$85.00Sep 30 – Oct 5
$95.00Oct 6 – Oct 6
5K Run/Walk ClosedOnline registration closed
$40.00Jan 1 – May 31
$45.00Jun 1 – Aug 31
$50.00Sep 1 – Sep 29
$55.00Sep 30 – Oct 5
$60.00Oct 6 – Oct 6
Two-Person Relay - TEAM CAPTAIN ClosedOnline registration closed
Relay captain will create and pay for the relay team. You do not need to register both relay members at this time.
$100.00Jan 1 – May 31
$105.00Jun 1 – Aug 31
$110.00Sep 1 – Sep 29
$120.00Sep 30 – Oct 5
ClosedOct 5 – Oct 6
Two-Person Relay - JOIN A TEAM ClosedOnline registration closed
Relay captain has already paid your entry fee. You must know your relay name and password to join the relay.
FreeJan 1 – Oct 5
ClosedOct 5 – Oct 6
Urban Cow Half Calf Kids' Races
Let your kiddos run with Howdy and pick up your Urban Cow bib and shirt at the Urban Cow Pre-Race Festival in William Land Park. Register your child for the Half Calf Kids' Races presented by Chick-fil-A on Saturday, October 5 at William Land Park. We have race distances for all kids ages 12 and under. Half Calf finishers receive a shirt, a Chick-fil-A treat, free race photos, and their own iconic cowbell medal.
Creating a H.E.R.D.
Grab Your Friends and Create a H.E.R.D.
Your HERD will be the funnest group in the race! But not just anyone can be in a HERD. Happy Enthusiastic Running Divas/Dudes means only your coolest friends and family members will make the cut to join yours.
A HERD is five or more people - because, let's face it, anything less than that is really just a couple of cows standing in a field! When you have at least five people in your HERD, everyone receives an invitation to our private packet pick up event on Thursday, October 3 sponsored by Fleet Feet Sports Sacramento, New Balance and Lagunitas. PLUS everyone's bib is personalized with the name of your HERD, so choose your name wisely! HERDs of 20 or more are allowed to set up a tent in the team area to meet before and after your run on race day.
It's time to get started! Create your HERD today when you register. Send out your HERD name to the coolest people you know and start training now. Don't wait for the fun to start on race day, plan some fun training runs (under a full moon, on Taco Tuesday, on some trails) and make it an experience everyone will remember.
- Personalized bib with team name (5 or more)
- Invitation to private packet pick up event sponsored by Fleet Feet Sports Sacramento (5 or more)
- Space for a team tent to meet before and after your run on race day (20 or more)
Event schedule
- October 5 9:00 AM PDT - Bib Pick-up at Pre-Race Festival
- October 6 6:15 AM PDT - Race Day Registration will start at 6:15am
- October 6 7:30 AM PDT - Half Marathon Wave 1 & Relay Start
- October 6 7:35 AM PDT - Half Marathon Wave 2 Start
- October 6 7:55 AM PDT - 5K Run/Walk Start
Contact information
- Name
- Kellie Haynes
- email@example.com
- Phone
- +1 916-492-8966


text:
Lot Details
Lot 165
American Silver Part Tea and Coffee Service
John Curry and Curry & Preston, Philadelphia, circa 1825
Comprising coffee pot, two teapots and cream jug, each globular-form chased with band of grapes and leaves, the scroll spouts with bird masks, with leaf capped handles; Together with a Silver-Plated Two-Handled Tray. Height of coffee pot 10 3/4 inches, total approximately 135 ounces, weighable.
C Property of a Long Island Estate
Additional Notes & Condition Report
Coffee pot - handle mark showing to interior; underside with traces of another inscription scratched out; handle slightly loose
Teapot 1 - handle mark showing to interior; chip loss to one insulator
Teapot 2 - handle mark showing to interior; handle slightly loose
Cream - with small bruises and crease dent; handle has been off at bottom and restuck
Tray 30 in. over handles; heavy wear to plating of surface
Any condition statement is given as a courtesy to a client, is only an opinion and should not be treated as a statement of fact. Doyle New York shall have no responsibility for any error or omission. The absence of a condition statement does not imply that the lot is in perfect condition or completely free from wear and tear, imperfections or the effects of aging.
No condition report? Click here to request one.


text:
Blogging in the Classroom: Conversation
Teaching workshops and books on pedagogy almost always recommend including more student participation/conversation in class – not exactly new advice, I know. I think most professors in Theology probably at least try to move beyond simply lecturing, but it is difficult to figure out how to do this consistently. This is particularly true in courses like the one I taught last Fall (forty students who are just fulfilling a university requirement). It is easy – at least it was for me when things got hectic – to simply prepare a lecture and run through the material rather than take the time to find a balance between lecture and real discussion or group work. One of the main reasons I included blogging in my course was that I hoped it would generate conversation among students before and during class.
The conversation among students on the blogs themselves (through the comments the were required to make within their 4-5 person blog group) was mixed. Especially at the beginning many of the comments did not really engage the original posts. They oftentimes just made a general affirmation and so no actual conversation ensued. Halfway through the course I addressed this and asked them to just make one comment within their group but to also make this comment more substantial in nature (respectfully critical or adding a new point to the original post). This helped a great deal. There were a number of times in the second half of the semester when I reshaped a point in my lecture or in-class discussion on the basis of a give-and-take on the blogs. In the future I think I will require two substantial comments (and these could include responses to comments on one's own blog).
By the end of the semester I was relatively happy with how the blogs functioned in terms of conversation before class, but I think the real potential of blogging is how they can affect conversation during class. First, the 4-5 person blog groups provide ready-made groups for in-class work. Students become accustomed to working with a group of students and they have faces to put on their core blog readers. Group discussions in class and on the blogs also mutually reinforce one another in creating a safe environment for students express their views and respond to others. Second, as I already indicated, threads of discussion or common difficulties on the blogs provide discussion points which are already active among the students. Since I knew before class what various students thought– both in their own posts and in response to others – it was much easier to focus on issues that were of direct interest to them and to address misinterpretations of the material. It also makes it easier to call upon students to share their analysis with the class – and I would try to do this even more in the future. This is especially helpful with students who have less background or who are more introverted (I remember one occasion in particular where a student who never spoke outside of his small group had made an outstanding point on his blog; it was great to be able to call on him during class in a way that empowered him to speak with confidence since he had thought through the idea beforehand).
Creating and maintaining thoughtful discussions in a relatively large introductory religion course is a difficult task. Blogging certainly does not solve the many issues which can lead to discussion being the exception rather than the rule. Nevertheless I think it can go quite far. In addition to simply being an interesting medium to students on its own, it gives them their own space to engage fundamental questions and ideas, it facilitates student dialogue through commenting, and provides a significant foundation from which in-class discussion can begin.
January 12, 2011 at 9:16 am


text:
Millennial Americans are getting tattoos more than any age group in the country, but a Hillsdale College student's research project suggests some tattoo inks may contain unsafe levels of certain metals.
A 2016 Harris poll found 47 percent of millennials have a tattoo, compared to only 29 percent of the population as a whole. Since the Food and Drug Administration does not regulate tattoo inks, many manufacturers are not transparent about the metals in their products.
Senior Matt Young said he decided to pursue a research project with Professor of Chemistry Mark Nussbaum due to the increasing popularity of tattoos and the lack of studies about toxic metals in tattoo inks.
"Tattoos seem to be a pretty prominent part of society now, and along with that, I wanted to get into doing research with metals and more inorganic materials," Young said.
Nussbaum said he decided to start this research project with Young after reading in Chemical and Engineering News about the possibility of harmful chemicals in tattoo inks.
"There isn't a lot of information available on the composition of tattoo inks since it hasn't been regulated in the past," Nussbaum said. "So, finding out what metals are present and at what levels is important and useful."
Young said an Italian study led to regulations on tattoo inks in the European Union, but no one in the United States had published a study with suggestions for safe metal levels.
Young analyzed the inks for six metals: cadmium, chromium, cobalt, lead, manganese, and nickel.
The European Commission recommended tattoo inks should not contain more than 0.2 parts per million of chromium, 1 ppm of manganese, or 2 ppm of lead. Though parts per million is a small amount, these low levels of metal in tattoo inks may still pose a safety risk.
Young's research suggested readily available tattoo inks in the United States greatly exceeded those levels. Young said ink from the Chinese manufacturer Solong Tattoo contains 15.4 ppm of manganese, a concentration he said is not potent but could lead to symptoms of manganese poisoning. He found the ink from the American manufacturer Eternal Ink contains 2.1 ppm of chromium.
He did not find elevated lead levels in the products he studied.
Young said he isn't opposed to tattoos after his research, but consumers should inform themselves about what they're putting on their bodies.
"Do research on your artists or personally ask them what brand of ink they're using," Young said. "That will give you a good idea of what sort of safety level you're at. Even with something that is made in the U.S. that's popular, you still don't know for sure be cause it's not regulated."
Nussbaum said the FDA should ensure tattoo inks are safe.
"Any commercial product which is intended to be injected into the body, even if only under the skin, should be regulated, in my opinion," he said. "The requirements for tattoo inks should be reasonable and based on known hazards, and it seems appropriate to me that the FDA, which regulates cosmetics, should also regulate the composition of tattoo inks."
After trying several different methods, Young and Nussbaum finally discovered a method that worked. They diluted tattoo ink, put it over a flame, and used the interaction between the light of the flame and the dissolved metal to determine the metal's concentration.
Young said it took two weeks to figure out how to get the tattoo ink to dissolve for further analysis.
"Matt did a very good job handling the frustration of dealing with a stubborn problem: how to get the tattoo inks dissolved in solution so that they could be tested in the flame atomic absorption instrument," Nussbaum said. "That experience and the way he handled it, while not part of our original plans, will serve him in good stead as he interviews for laboratory positions in the real world, where troubleshooting and patient persistence are often necessary."


text:
Hey folks! Today I'm happy to introduce you to Adriana Kraft and their story Seducing Cat!
Winner of the 2014 Bisexual Book Award for erotic fiction, author Adriana Kraft is a married couple writing Sizzling Romantic Suspense and Erotic Romance for Two, Three, or More.
One man, one woman, danger and intrigue – always a happy ending, but oh, what a ride! Readers can count on our Romantic Suspense line for gutsy characters, hot sex, and breathtaking intimacy as our hero and heroine battle outer threats and inner demons to stay alive and fall in love.
A man, a woman (or two), or another man, threesomes, foursomes—what's your fantasy? We write our Erotic Romance stories to entertain, of course, but most of all we write them because we believe in happy endings for all who fall in love, whatever their gender, sexual orientation or numerical combination. Here you'll find multiple partners, three-way, four-way and more, swing lifestyle, lesbian, bisexual, ménage and polyamory, in both contemporary and paranormal settings.
Together we have published over thirty-five romance novels and novellas to outstanding reviews.
Website ## Blog ## Amazon Author Page ## Twitter ## Facebook ## Newsletter ## Pinterest ## Goodreads ## eMail
~ * ~ Guest Post From Adriana Kraft ~ * ~
What lengths would you go to if you wanted to seduce someone? What if the woman you want to seduce is a frosty English professor older than you by at least a decade—and what if, in addition to that, you want to get her hooked up with your current lover, a brawny carpenter?
Believe it or not, that's Meg's plan, and there's hardly any length she won't go to. She's only twenty, and the small-town world of Burntside, Wisconsin, is her apple. She stars in the local playhouse productions – directed, it turns out, by that same frosty English professor. Her goals? Have fun, have lots of uninhibited sex, enjoy the moment, and avoid any commitments that limit her freedom. She's convinced that hooking Cat up with Kurt will bring them pleasure and set her free to pursue her next dream somewhere far away from Burntside.
In a nutshell, that's her motivation. Our motivation in creating her? We set out to write a romp, not worrying too much at first about depth of character or character transformation. We crafted the broad strokes of a plot in which we could deliver some of our own favorite kinds of erotic scenes. But both we and our characters discovered deeper motivations as we delved into the project.
We'll let readers discover how Meg grows and changes across the series, which covers nearly a decade of her life. We think she may surprise you! Our goals expanded beyond delivering hot sex scenes to exploring, in this book, how both a carpenter and a professor can shed their negative assumptions about each other and revel in the things it turns out they have in common. Much of the rest of the series also focuses on destroying stereotypes and blowing up assumptions, while never losing sight of creating sizzling mind-blowing ménage scenes. We think that makes for some surprising scorching combinations.
This five book series was originally released in 2009 by a different e-publisher. It's been unavailable for over four years, and we're thrilled Extasy Books has contracted to re-release it, completely revised and re-edited. In the meantime, Meg insisted we write one more adventure. The six books will release approximately every ten weeks. Watch for Book Two: A Woman for Zachary in early August!
In the excerpt below, Meg's been sunbathing nude in the back yard of the house where she rents a room from the professor, the first steps in her seduction plan.
Level headed English Professor Caitlin Shanahan has finally earned tenure and purchased the century-old two-story house of her dreams in the college town of Burntside, Wisconsin—at thirty-four, she'd better face facts: her future is teaching college and writing plays, not falling in love. So why is she so drawn to foxy little hometown actress Meghan Keenan, who's rented her upstairs apartment, or worse, to the girl's gorgeous hunk of a lover Kurt Davis, a carpenter who's never even been to college?
Twenty-year-old Meg has a plan. First she'll seduce the reluctant professor, then she'll share her sexual delights with both Cat and her brawny carpenter, and when she's successfully brought them together, she can make her escape and explore what's beyond the only town she's ever lived in.
Kurt can't stand the up-tight professor who's hired him to build a gazebo and refurbish her old house—the house he wanted for himself. Even if Meg's scheme succeeds, he knows Caitlin would never look at him twice. No need to go there in his fantasies…
Peering through shuttered eyelashes, Meg Keenan nearly purred. She'd seen the tall figure of Cat Shanahan leave the curtained window. Apparently the lanky redhead had no idea the sun struck the window in a way that showed her slim form off to nice advantage.
Meg snickered. Why else would she choose this particular time of day to come out onto the sundeck? Because the sun shining on the window exposed Cat to her view almost as much as she was exposed to Cat's.
At least she had the woman's attention. Meg closed her eyelids and exhaled slowly. Soon she'd have Cat's body.
Stretching languidly, Meg smiled and sat up. Her plan was proceeding quite nicely. Kurt had been pissed she'd rented the professor's upstairs apartment. Though Meg was quite comfortable being a townie, Kurt carried a chip on his shoulder about town-gown tensions. Was that part of why he'd never gone to college in spite of doing so well in high school? Instead, he and Jack Mobly had established a construction company. At twenty-four, Kurt had a certain amount of control over his life that Meg envied. He didn't seem to care that there was a world outside Burntside, Wisconsin.
She, on the other hand, needed to see beyond the horizon. She hadn't found her ticket out just yet, but she knew she'd find a way. Fortunately, they both knew that.
While she and Kurt had been lovers for two years, neither suffered under the illusion of any long-term commitments. She'd known him all her life. And they were very compatible lovers. What one partner didn't think of, the other did, and being together meant they could enjoy their fascination with sex without having to deal with the meat market. Their relationship was one of convenience, yet quite satisfying.
But Meg was ready to leave Burntside, and she wanted Kurt to have something he didn't even know he wanted. She wanted him to have Professor Caitlin Shanahan. And she'd given herself the assignment to make that happen.
Extasy Books ## Amazon ## Barnes & Noble ## Kobo ## Smashwords
Adriana Kraft will be awarding a $10 Amazon GC, to a randomly drawn winner via rafflecopter during the tour. CLICK HERE to enter to win! Readers, follow the tour and comment; the more you comment, the better your chances of winning. The tour dates and places can be found here: Tour Schedule
~ * ~
Adriana Kraft, thank you for stopping by today!
Love & blessings to all! ❤
Tagged: Adriana Kraft, anal sex, Book Spotlight, Erotic, erotic romance, explicit sex, eXtasy Books, FF, FFM, FMF, Heat Level Four flames, Interview, M/f, Menage, menage scenes, Reviews by Crystal, Romance, sex toys, voyeurism


text:
Chronic inflammation can be an essential process resulting in tumorigenesis. zebrafish. Molecular characterization exposed upregulation from the downstream parts mixed up in IL6-mediated signaling pathways, specifically PI3K/Akt and JAK/STAT3 pathways. Additional analysis indicated that PI3K was the most reactive towards the infiltrated inflammatory cells and dysplasia with huge cell modify, whereas STAT3 was greatly activated in your community with dysplastic foci, recommending that this JAK/STAT3 pathway was primarily implicated in the hepatic tumorigenesis in today's model. Our present research provides an proof of the partnership between chronic swelling and tumorigenesis and reinforces the pivotal part of IL6 in the inflammation-associated hepatocarcinogenesis. proof that hepatic manifestation from the hIL6 induces the persistent inflammation resulting in hepatocarcinogenesis. Components and Strategies Transgene Constructs and Transgenesis All constructs found in our research had been sequenced and confirmed using the correct primers outlined in Supplementary Desk S1. For transgenesis, the transgene constructs p(LFABP:Gal4VP16), p(UAS:RFP), and p(UAS:hIL6,Cmcl2:GFP) had been separately produced (Physique?1A). Quickly, a 2.8-kb upstream region from the liver-specific LFABP gene was polymerase string response (PCR)Camplified as referenced with a earlier report [17] and utilized as the promoter to operate a vehicle Gal4VP16 gene in the zebrafish liver organ. The hIL6 cDNA bought from Open up Biosystems (Huntsville, AL) was PCR-amplified and cloned in to the downstream of UAS promoter. After that, Cmcl2-GFP (for cardiac manifestation of GFP) was PCR-amplified and cloned to create p(UAS:hIL6,Cmcl2:GFP). The p(UAS:RFP) was made by putting the RFP series amplified from pAsRed2 (Invitrogen, Carlsbad, CA) beneath the pUAS promoter. Make reference to Supplementary TAK-438 Desk 1 for primer sequences. Open up in another window Physique?1 Transgenic strategy and particular expression of hIL6 gene in TAK-438 transgenic zebrafish. (A) Framework from the constructs found in the TAK-438 Tol2-mediated transgenesis. (B and C) Embryo pictures at 4 dpf (still left, merged pictures) demonstrated RFP appearance in the liver organ. Hepatic appearance of RFP and cardiac appearance of GFP had been utilized as the indications to choose the transgenic embryos under a fluorescence microscope. ISH for hIL6 (middle) at 4 dpf demonstrated its RNA appearance in the liver organ (dark arrowhead). IHC at 6 weeks (correct) displaying the hIL6 appearance on the hepatocytes just in and strains, the transgenic zebrafish expressing hIL6 particularly in the liver organ, i.e., Hybridization (ISH) Histologic evaluation was completed by planning 4-m transverse areas from 4% paraformaldehyde-fixed, paraffin-embedded tissues. Hematoxylin and eosin (H&E) staining was performed based on the regular process [19]. IHC and ISH tests were completed as previously referred to [20]. Major antibodies found in the tests had been rabbit anti-IL6 (1:200), rabbit anti-caspase 3 (1:100), mouse anti-proliferating cell nuclear antigen (PCNA) (1:2000), and rabbit anti-JAK1 (1:200) from Abcam (Cambridge, MA). Mouse anti-phospho-PI3K (1:100) was bought from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Rabbit antibodies for phospho-Tuberin/TSC2 (1:200), phospho-mTOR (1:200), phospho-4EBP1 (1:200), phospho-RS6K (1:200), and phospho-STAT3 (1:200) had been bought from Cell Signaling (Danvers, MA). For ISH test, incomplete cDNA sequences had been PCR-amplified using the correct primers (make reference to Supplementary Desk 2) and cloned into pCRII vector (Invitrogen). Riboprobes had been generated using T7 or SP6 digoxigenin labeling package (Roche Diagnostics GmbH, Mannheim, Germany). Hybridization was completed at 65C TAK-438 right away, and some stringent clean was completed at 68C. Hybridized riboprobes had been discovered by anti-dig antibody binding and visualized by incubating with an NBT/BCIP AP substrate option (Roche Diagnostics GmbH). Counterstaining was finished with natural reddish colored. Imaging Olympus MVX10 was useful for whole-mount embryo imaging. Photos from slide areas were attained using an Olympus BX51. Change transcriptase (RT)CPCR and Traditional western Blot Analyses Real-time RT-PCR was performed utilizing the entire liver tissues dissected from 3-month-old zebrafish. For every group, RNA test was extracted through the use of TRIzol reagent (Invitrogen). cDNA was synthesized with a Maxima Initial Strand cDNA Synthesis Package (Thermo Scientific Fermentas, K1641, Glen Burnie, MD). The RT-PCR was performed through the use of Maxima SYBR Green/ROX qPCR Get better at Combine (Thermo Scientific Fermentas, K0222) on the 7300 Real-Time PCR Program (Applied Biosystems, Foster town, CA). Primer sequences for the RT-RCR are proven in Supplementary Desk 3. All tests were repeated 3 x with individually ready examples. Statistical significance was examined with the Mann-Whitney check using SPSS 11 software program. For Traditional western blot assay, entire cell extracts had been ready from zebrafish liver TPOR organ as referred to previously [21]. Twenty micrograms of every test was separated on the 10% SDSCpolyacrylamide gel and moved onto a polyvinylidene difluoride membrane (Amersham, GE Wellness, Sweden). The membrane was incubated right away at 4C with major antibody within a PBS preventing solution (non-fat dry dairy). Horseradish peroxidaseCconjugated supplementary antibody was useful for post reaction. Tagged proteins were after that discovered by ECL.
Category: Carbonic Anhydrases
"type":"entrez-nucleotide","attrs":"text message":"LY294002″,"term_identification":"1257998346″,"term_text message":"LY294002″LY294002 is a man made quercetin-like substance which, unlike
"type":"entrez-nucleotide","attrs":"text message":"LY294002″,"term_identification":"1257998346″,"term_text message":"LY294002″LY294002 is a man made quercetin-like substance which, unlike wortmannin, can be an inhibitor of phosphatidylinositol 3-kinase (PI3K). dismutase. This probably happened via PI3KCmediated downregulation of proteins AtDJ1A. Finally, Rabbit Polyclonal to IARS2 we discuss specificity distinctions of "type":"entrez-nucleotide","attrs":"text message":"LY294002″,"term_id":"1257998346″,"term_text message":"LY294002″LY294002 and wortmannin against PI3K that are reflected on the proteome level. In comparison to wortmannin, "type":"entrez-nucleotide","attrs":"text message":"LY294002″,"term_id":"1257998346″,"term_text message":"LY294002″LY294002 showed even more narrow as well as perhaps also even more specific results on protein as recommended by gene ontology useful annotation. protoplasts.12 However, there's also some differences between your ramifications of wortmannin and "type":"entrez-nucleotide","attrs":"text message":"LY294002″,"term_identification":"1257998346″,"term_text message":"LY294002″LY294002. Unlike wortmannin, "type":"entrez-nucleotide","attrs":"text message":"LY294002″,"term_id":"1257998346″,"term_text message":"LY294002″LY294002 will not inhibit the first levels of endocytosis (as assessed by uptake from the styryl dye FM 1C43), while adversely impacting the fusion lately endosomes with tonoplast in main hairs.13 In another research, wortmannin completely abolished staining and reduced the amount of endosomes visualized by FYVE-DsRed in transiently transformed detached leaves of root base.4 Cell biological approaches validated proteomic data on RabA1d, a little Rab GTPase localized to early endosomes, and downregulated by fusion of early endosomes with past due endosomes after wortmannin treatment. These outcomes showed the feasibility of mixed proteomic and cell natural methods to research physiological adjustments in root base due to pharmacological perturbation of PI3/4K signaling. With this research, we performed a proteomic evaluation of origins using a even more particular inhibitor of PI3K, specifically "type":"entrez-nucleotide","attrs":"text message":"LY294002″,"term_id":"1257998346″,"term_text message":"LY294002″LY294002. We targeted to integrate buy 172889-27-9 proteomic data buy 172889-27-9 using the subcellular results (e.g. on endosomes and antioxidant activity) due to this inhibitor in main cells. Inhibitor results at subcellular level had been validated by live-cell imaging, and proteomic data on upregulation of 2S albumin had been validated by both immunoblots and immunolabelling on undamaged origins. Most obvious commonalities and variations between previously reported wortmannin results and "type":"entrez-nucleotide","attrs":"text message":"LY294002″,"term_id":"1257998346″,"term_text buy 172889-27-9 message":"LY294002″LY294002 are talked about. Experimental procedures Vegetable materials and cultivation Seed products of (ecotype Col-0 and transgenic Col-0 lines with PI3P and endosomal molecular markers) had been surface area sterilized and positioned on half-strength MS lifestyle moderate (pH 5.7) containing 1% (w/v) sucrose and 0.8% (w/v) phytagel. The plates had been kept at 4C for 48 h to break dormancy, and held vertically under 16 h light/8 h dark, 22C circumstances, for 10 times (proteomic evaluation) or 6 times (microscopic evaluation). Seedlings had been surface-treated with liquid ? MS mass media filled with 33 M "type":"entrez-nucleotide","attrs":"text message":"LY294002″,"term_id":"1257998346″,"term_text message":"LY294002″LY294002 (Sigma) dissolved in dimethylsulfoxide (DMSO; last focus 0.3% DMSO [v/v]) for 2 h, while stopping complete submergence from the root base in the water. Control plants had been treated with alternative containing ? MS moderate supplemented with 0.3% DMSO. Root base had been quickly dissected and gathered for proteins extraction. The introduction of transgenic lines employed for microscopic evaluation was defined previously.17 As PI3P marker, and late endosomes marker we've used series stably expressing the GFP-tagged increase FYVE build, and series expressing YFP-tagged RabF2a,17 respectively. Proteomic evaluation Protein removal for two-dimensional electrophoresis and 2-D LC-MS/MS Protein were extracted based on the process of Tak? et al.18 Briefly, root base had been frozen in buy 172889-27-9 water nitrogen and homogenized to an excellent powder utilizing a mortar and pestle. Phenol was employed for total proteins extraction regarding to a improved approach to Hurkmann and Tanaka.19 An in depth description from the protein extraction method is supplied in the supplemental data. Two-dimensional electrophoresis The 2-DE was performed as released in our prior research.18 Details are given in supplemental data. Ingredients from plant materials gathered from three unbiased biological tests (3 natural replicates) were employed for.
Objectives To examine adherence to serum creatinine and potassium monitoring and
Objectives To examine adherence to serum creatinine and potassium monitoring and discontinuation recommendations subsequent initiation of treatment with ACE inhibitors (ACEI) or angiotensin receptor blockers (ARBs); and whether high-risk sufferers are supervised. and 47% both at baseline and follow-up. The median period between your latest baseline monitoring and medication initiation was 40?times (IQR 12C125?times). 34% of sufferers acquired baseline creatinine monitoring within 1?month before initiating therapy, but 10% also had the guideline-recommended follow-up check recorded within 2?weeks. Among sufferers suffering from a creatinine boost 30% (n=567, 1.2%) or potassium level 6?mmol/L (n=191, 0.4%), 80% continued treatment. Although sufferers with preceding myocardial infarction, hypertension or baseline potassium 5?mmol/L were in risky of 30% upsurge in creatinine after ACEI/ARB initiation, there is no proof that these were more often monitored. Conclusions Just one-tenth of sufferers initiating ACEI/ARB therapy have the guideline-recommended creatinine monitoring. Furthermore, almost all the patients satisfying postinitiation discontinuation requirements for creatinine and potassium boosts keep on treatment. when the finish time from the first constant span of therapy was following the time from the first monitoring time plus 30?times (to permit for stockpiling). The finish time of every prescription was computed with the addition of the prescription duration WYE-132 (final number of tablets recommended divided with the specified variety of tablets each day) towards the prescription time. In identifying constant classes of therapy, we allowed for the 30-day gap between your end time of 1 prescription and the beginning of another consecutive prescription. In awareness analyses, we repeated WYE-132 WYE-132 the analyses (1) increasing the follow-up screen for the initial follow-up monitoring from 2-3 3?weeks to take into account small delays; (2) including just the newest calendar period (2009C2014) to take into account temporal adjustments in data completeness and quality of treatment; (3) excluding individuals with a medical center admission or release day within 1?month before or after their initial ACEI/ARB prescription, to be able to account for medication initiation and any subsequent renal function testing occurring in a healthcare facility and for that reason not captured in the CPRD; (4) concentrating on particular individual subgroups (center failing, myocardial infarction, hypertension, CKD (eGFR 60?mL/min/1.73?m2), peripheral arterial disease and diabetes); and (5) defining medication make use of WYE-132 continuation as ACEI/ARB make use of beyond 90?times (rather than 30?times) following the initial retest day. We utilized the subcohort of individuals PP2Abeta with both baseline and follow-up monitoring to calculate the percentage of individuals with creatinine raises 30% or potassium amounts 6?mmol/L in the first follow-up monitoring within 2?weeks after initiation, aswell as the percentage of individuals continuing treatment in spite of these contraindications for make use of. Finally, we installed a logistic regression model to recognize patient characteristics connected with a serious decrease in renal function (creatinine boost 30% or potassium level 6?mmol/L) and compared these features with those connected with receiving postinitiation follow-up monitoring within 2?weeks. The model included age group, sex, CKD stage, cardiovascular comorbidities, diabetes and baseline potassium level ( 5 vs 5?mmol/L). In three extra model-based level of sensitivity analyses, we repeated the WYE-132 analyses (1) excluding individuals with a recently available hospitalisation (as described above); (2) omitting baseline potassium from your model to examine the degree of potential overfitting when both baseline potassium and CKD stage had been held in the model; and (3) also adjusting additionally for ethnicity. All analyses had been performed using the STATA 14 statistical program. Outcomes Serum creatinine monitoring before and after ACEI/ARB initiation We recognized 223?814 new users of ACEI/ARB. We likened these individuals in four organizations: 21?411 (10%) had zero baseline or follow-up creatinine assessments within 12?weeks before and 2?weeks after treatment initiation, 63?359 (28%) experienced only set up a baseline test, 33?185 (15%) experienced only follow-up tests, and 105?859 (47%) experienced both baseline and follow-up tests (table 1). Median age group varied only somewhat between the organizations (60, 62, 59 and 63?years, respectively) and there have been no substantial variations in socioeconomic position, lifestyle elements or peripheral arterial disease. Weighed against individuals with neither preinitiation nor postinitiation monitoring, individuals with both had been much more likely to possess diagnosed hypertension (76% vs 61%) and diabetes (20% vs 7%), but less inclined to have diagnosed center failing (4% vs 7%), myocardial infarction (4% vs 18%) and arrhythmia (7% vs 10%). Among individuals with baseline monitoring, 83% didn't possess CKD, 13% stage 3a, 3% stage 3b, 0.5% stage 4 CKD. In the same populace, 7% began ACEI/ARB therapy despite.
Huntingtons disease (HD) can be an autosomal dominant inherited and progressive
Huntingtons disease (HD) can be an autosomal dominant inherited and progressive neurodegenerative disorder with engine dysfunction and cognitive deficits. activity reliant neurotrophic element (ADNF) and activity reliant neuroprotective proteins (ADNP). The discharge of vasoactive intestinal peptide (VIP) stimulates glial focus on receptor and induce vesicular excytosis included ADNF and ADNP. ADNF and ADNP maintain neuronal success in neurodegenerative illnesses through unknown system. The pharmacological activities of neurotrophic elements are considered appealing new healing agents for the treating HD. There are in least three neurotrophic elements which have been examined in pre-clinical and scientific settings for the treating the development of HD. The final results of the neurotrophic elements are discussed within this review. There's also various other neurotrophic factors examined in various other neurodegenerative diseases that could be regarded Dihydrotanshinone I supplier potential medications for the treating HD. 2.1. BDNF BDNF is available to become a significant trophic aspect for the treating HD. It really is noteworthy that the amount of BDNF is available to become downregulated in HD sufferers [28C30]. Relating, downregulation of BDNF was discovered to become connected with CAG repeats [31]. Deficit in BDNF amounts is connected with alteration of Dihydrotanshinone I supplier BDNF transportation by mutant huntingtin proteins [32, 33]. Generally, normal huntingtin proteins is found to improve vesicular transportation Rabbit Polyclonal to p63 of BDNF along microtubules, but mutant huntingtin can transform this system. Regulating the degrees of BDNF in the corticostriatal pathway might promote cell success and consequently hold off the development of HD. BDNF was discovered to become stated in cortex and carried in the corticostriatal pathway in the moderate spiny neurons [34, 35], which will be the neurons most suffering from HD. This shows that healing approaches concentrating on the boost of BDNF amounts may be a potential technique to gradual the development of HD (for review discover Guide [18]). BDNF provides been shown to become linked mechanistically using the root hereditary defect in HD (for review discover Ref. [36]). BDNF is recognized as a potent aspect to avoid cell loss of life, as shown also to hold off the development of HD, as proven in pet versions [31, 37C41]. Research have assessed the consequences of upregulation of BDNF using chemically induced disease. Hence, delivery of BDNF by proteins infusion, intrastriatal shot of adenovirus expressing BDNF, or implantation of cells expressing BDNF induced neuroprotection in striatum that was subjected to poisons [41C43]. Moreover, research using HD mouse versions demonstrated that BDNF can be neuroprotective [7]. Hence, BDNF administration reversed the elevated of GABAergic function within HD mouse versions [44]. The delivery of BDNF using osmotic minipump in to the striatum in mice overexpressing exon 1 of individual mutant huntingtin proteins was connected with raised appearance of encephalin, which can be affected mainly in HD [31]. This research also demonstrated postponed electric motor impairment and expanded success amount of time in these pet versions. Another study utilizing a mix of BDNF-adenovirus vector delivery and noggin molecule demonstrated marketing neurogenesis, striatal neuronal regeneration, and postponed electric motor impairment and expanded the success amount of time in HD mouse versions [45]. Just like HD, BDNF can be a potential neurotrophic aspect for treatment of Advertisement. Deficits of cholinergic neurons are most likely the reason behind cognitive deterioration, which is among the main symptoms of Advertisement [21]. The usage of BDNF in Advertisement works more effectively for ameliorating the cholinergic features [46]. Furthermore, BDNF mediates synaptic plasticity and cognitive function [47]. In human beings suffering from Advertisement, BDNF mRNA and proteins were found to become reduced in cholinergic neurons in the cortex and hippocampus. A deficit in pro-BDNF proteins also was within the parietal cortex in Advertisement [48]. It really is clear that this reduced amount of BDNF amounts in Advertisement, especially in cholinergic neurons, shows that neurotrophic factor is known as a key element in Advertisement. The acetylcholinesterase inhibitors, antioxidants, and glutamate antagonists have already been used mainly in Dihydrotanshinone I supplier clinical configurations for the treating Advertisement [49C51]. A patent pertains to book analogs of choline and ways of make use of for treatment of Advertisement, HD and additional neurodegenerative illnesses [52]. Collectively, these findings offer ample information regarding the uses of BDNF in a number of neurodegenerative illnesses including HD. Although, the system of actions Dihydrotanshinone I supplier of BDNF in preventing.
The role of endogenous tachykinins on guinea-pig colonic propulsion was investigated
The role of endogenous tachykinins on guinea-pig colonic propulsion was investigated through the use of potent and selective tachykinin NK1 and NK2 receptor antagonists. the result was significant limited to Males 10627 (+84%). SR 48968 (1?mol?kg?1, i.v.) didn't significantly improve the colonic NIBR189 propulsion. non-e of the tachykinin NK2 receptor antagonists altered the amplitude of colonic contractions. On the other hand, both atropine (6?mol?kg?1, i.v., plus infusion of just one 1.8?mol?h?1) and hexamethonium (55?mol?kg?1, i.v., plus infusion of 17?mol?h?1) abolished propulsion (81% and 87% inhibition, respectively) and reduced the amplitude of contractions (68% inhibition for either treatment). In atropine-treated pets (6?mol?kg?1, i.v., plus infusion of just one 1.8?mol?h?1), apamin (30?nmol?kg?1, i.v.) restored colonic propulsion (+416%) and improved the amplitude of contractions (+367% when compared with atropine only). Hexamethonium (55?mol?kg?1, i.v., plus infusion of 17?mol?h?1) NIBR189 SDF-5 abolished the apamin-induced, atropine-resistant colonic propulsion (97% inhibition) and decreased the amplitude from the atropine-resistant contractions (52% inhibition). The apamin-induced, atropine-resistant colonic propulsion was inhibited by SR 140333 (?69% at 1?mol?kg?1), SR 48968 (?78% at 1?mol?kg?1), Males 11420 (?59% at 1?mol?kg?1) and Males 10627 (?50% at 1?mol?kg?1), even though latter effect had not been statistically significant. The mixed administration of SR 140,333 and Males 10,627 (1?mol?kg?1 for every antagonist) almost completely abolished colonic propulsion (90% inhibition). The amplitude of colonic contractions was also decreased by SR 140333 (?42%), NIBR189 SR 48968 (?29%), MEN 11420 (?45%) however, not by MEN 10627 (?16%). The mixed administration of SR 140333 and Males 10,627 decreased the amplitude of contractions by 47%. SR 140603 (1?mol?kg?1, i.v.), the much less potent enantiomer of SR 140333, was NIBR189 inactive. In charge pets, apamin (30?nmol?kg?1, i.v.) improved colonic propulsion (+84%) and improved the amplitude of contractions (+68%), when compared with the automobile. Hexamethonium (55?mol?kg?1, i.v. plus infusion of 17?mol?h?1) inhibited propulsion (86% inhibition) and decreased the amplitude of contractions (49% inhibition). SR 140333, SR 48968, Males 11420, Males 10627, or the coadministration of SR 140333 and Males 10627 experienced no impact. In another series of tests, the imply amplitude of colonic contractions was also documented under isovolumetric circumstances through the balloon-catheter gadget kept set up at 75?mm from your rectal sphincter (static model). In charge circumstances, neither SR 140333 nor Guys 11420 customized the amplitude of contractions. In atropine-pretreated guinea-pigs, SR 140333 and Guys 11420 (0.1C1?mol?kg?1) dose-dependently decreased the amplitude of contractions. In apamin- and atropine-pretreated pets, only the best (1?mol?kg?1) dosage of SR 140333 or Guys 11420 significantly decreased the amplitude of contractions. NIBR189 The inhibitory strength of atropine (0.3C1?mol?kg?1) was equivalent in apamin-pretreated pets and in handles. It was figured, in anaesthetized guinea-pigs, endogenous tachykinins, performing through both NK1 and NK2 receptors, become non-cholinergic excitatory neurotransmitters to advertise an apamin-evoked reflex propulsive activity of the distal digestive tract. strong course="kwd-title" Keywords: Apamin, atropine, digestive tract, hexamethonium, Guys 10627, Guys 11420, SR 48968, peristalsis, SR 140333, tachykinin antagonists Total Text THE ENTIRE Text of the article is obtainable being a PDF (337K)..
Mitochondrial potassium channels are essential mediators of cell protection against stress.
Mitochondrial potassium channels are essential mediators of cell protection against stress. particular, safety against ischemia-reperfusion (IR) damage is definitely thought to continue via starting of mitochondrial K+ stations [1]. Many cardioprotective strategies need such stations, and route opening alone is enough to induce safety [2], [3]. For instance, the safety by ischemic preconditioning entails the mitochondrial ATP-sensitive K+ (mKATP) route and activation from the route is definitely cardioprotective [2], [3], [4]. Likewise, volatile anesthetics protect the center against IR damage, in a trend termed anesthetic preconditioning (APC) [5], [6]. APC is definitely evolutionarily conserved from to mammals [7], and it is potentially of medical importance [6]. The complete systems of APC remain elusive, although mitochondrial Ca2+ turned on K+ channels have already been suggested as mediators [8]. The canonical cell surface area large-conductance, big K+ (BK) route is definitely coded for from the gene in worms and by the (gene in worms and by two genes ((in addition has been recognized, its expression is UK-427857 fixed to mammalian spermatozoa [14]. The purpose of this research was to mix the energy of genetics with mouse center physiology and isolated mitochondrial assays, to research the comparative contribution of SLO1 and SLO2 to mBK root APC. Right here, we present proof UK-427857 assisting an evolutionarily conserved part for SLO2 in facilitating mitochondrial K+ transportation resulting in APC. Methods Total experimental information are in Assisting Info S1. C. elegans Strains found in this research consist of Bristol-N2 (wild-type, WT); NM1968 Hereditary Center and had been either acquired backcrossed or had been backcrossed onto an N2-Bristol history. Solitary worm PCR genotyping was utilized to check out the mutant alleles. Mice Characterization from the mBK route was performed using male wild-type (WT) C57BL6 mice age group 6C8 weeks bought from your Jackson Lab (Pub Harbor, Me personally). Experiments including as previously explained [16]. Mitochondria had been isolated from mouse hearts as previously explained [17]. The mitochondria had been incubated with 20 M BTC-AM and 0.05% Pluronic F-127 for 10 min at room temperature. The ultimate mitochondrial pellet was suspended in 225 l from the particular isolation medium explained in Supporting Info S1 and kept on snow until make use of, within 1.5 hrs. Proteins was dependant on the Folin-phenol technique [18]. mBK thallium flux assay (Tl+-flux) Tl+ is definitely a surrogate for K+ as well as the Tl+-flux assay is definitely trusted in the mobile K+ route field [19]. Tl+ uptake into mitochondria had been measured utilizing a Varian Cary Eclipse spectrofluorometer as previously explained [20] by monitoring adjustments in BTC fluorescence (ex 488 nm, em 525 nm). Mouse Langendorff perfused center Pursuing anesthesia with avertin, a rodent 3-business lead EKG was briefly acquired. A thoracotomy was after that performed, as well as the aorta cannulated and quickly used in a perfusion equipment, as previously defined [17]. The center was perfused with Krebs-Henseleit buffer using continuous flow and subjected to global ischemia, as comprehensive UK-427857 in Supporting Details S1. Pursuing experimental protocols, hearts had been stained and imaged as previously defined to quantitate infarct size [21]. Reagents All chemical substances were of the best grade obtainable from Sigma (St. Louis, MO) unless usually given. Iberiotoxin, Charybdotoxin, and Apamin had been from EMD Chemical substances Group (Darmstadt, Germany); Bepridil and Paxilline had been from Enzo Lifestyle Sciences International, Inc. (Plymouth Reaching, PA); Bithionol was from TCI America (Portland, OR); BTC-AM, Benzothiazole coumarin acetyoxymethyl ester was from Invitrogen (Carlsbad, CA). Figures Data provided are mean SEM. Statistical significance (P 0.05) between multiple groupings was determined using evaluation of variance (ANOVA). Entirely worm research, significance (P 0.05) was determined utilizing a paired Student's isoforms in conferring K+ transportation over the ZNF538 mitochondrial inner membrane, a recently developed fluorescent assay [20] was put on isolated mouse center mitochondria, where thallium (Tl+) flux acts as a K+ surrogate. Tl+, unlike K+, isn't normally within the mitochondrial matrix therefore UK-427857 does not need depletion before the assay. Furthermore, Tl+ is certainly transported very successfully by K+ stations. Together with transporter-specific pharmacologic signatures, this system allows for evaluation UK-427857 of pathways that donate to mitochondrial K+ transportation. For instance, we shown previously that mKATP stations donate to Tl+ flux.
Memory deficits are normal among stroke survivors. AMPK signaling as the
Memory deficits are normal among stroke survivors. AMPK signaling as the protecting ramifications of GSK-3 inhibition had been observed in AMPK deficient mice. Nevertheless, GSK-3 inhibition offered no additive safety in mice treated having a TAK inhibitor recommending that TAK1 can be an upstream regulator of GSK-3. Focusing on GSK-3 is actually a book therapeutic technique for post-stroke cognitive deficits. Heart stroke is the main reason behind long-term adult impairment and the 4th leading reason behind death in america (Feigin et al. 2003; Lloyd-Jones et al. 2010; Vaartjes et al. 2013). Ischemic strokes makes up about 80%C85% of most strokes (Proceed et al. 2014). Regardless of the global burden of heart stroke, only 1 FDA-approved therapy is definitely available to deal with ischemic heart stroke individuals, Ivacaftor the thrombolytic cells plasminogen activator (Ziegler et al. 2005). tPA can only just be utilized in a small % of patients because of its brief therapeutic time screen and many contraindications (Ziegler et al. 2005). As our people age range the prevalence and occurrence of cerebrovascular disease will continue steadily to boost (Lloyd-Jones et al. Ivacaftor 2010; Vaartjes et al. 2013), as will the amount of people with post-stroke cognitive deficits. While medical center costs take into account three-fourths of total heart stroke treatment costs, the expense of long-term chronic treatment is normally a major financial concern. Heart stroke survivors with physical or cognitive impairments frequently need community-based treatment or nursing house positioning. No neuroprotective realtors have demonstrated advantage in clinical studies, recommending the growing have to explore book pathways and focuses on. Glycogen synthase kinase-3 (GSK-3) can be an evolutionary conserved ubiquitous serine/threonine kinase comprising two specific isoforms, GSK-3 and GSK-3 (Liang and Chuang 2007). It really is a multifaceted proteins that is extremely indicated in the mammalian mind and involved with diverse mobile and neurophysiological features (Chuang et al. 2011). Probably one of the most significant characteristics of GSK-3 may be the multitude of signaling pathways that converge onto it, recommending that it might be an important natural focus on (Forde and Dale 2007; Miura and Miki 2009). GSK-3 is definitely constitutively energetic under normal relaxing circumstances (Peineau et al. 2008). An evergrowing body of proof indicates that triggered GSK-3 is definitely pro-apoptotic (Jend?elovsky et al. 2012). GSK-3 is definitely inactivated by phosphorylation at Ser9 (McManus et al. 2005; Chuang et al. 2011). Dysregulation of GSK-3-mediated substrate phosphorylation and signaling continues to be implicated in a number of pathophysiological circumstances including tumor (Luo 2009), Alzheimer's disease (Engel et al. 2006), diabetes (Eldar-Finkelman et al. 1999), and feeling disorders (Li and Jope 2010). GSK-3 works as a regulator of apoptosis and swelling, known contributors to stroke-induced cell loss of life (Gao et al. 2008). Lack of GSK-3, not really GSK-3, suppressed spontaneous neuronal loss of life in extended tradition versions (Liang and Chuang 2007). non-selective GSK-3 inhibition with lithium is definitely neuroprotective (Chuang et al. 2011; Wei et al. 2013) and GSK-3 inhibitors are being analyzed in clinical tests for treatment of cognitive deficits and dementia (Hong-Qi et al. 2012). GSK-3 may connect to the mitogen-activated proteins kinase family members (MAPKs) and promotes signaling after tension (Kim et al. 2003). Changing development factor–activated kinase-1 (TAK1) is definitely a member from the MAPK family members that is also called mitogen-activated proteins kinase kinase kinase-7. TAK1 is definitely triggered by TGF-, tumor necrosis element- (TNF-), and additional cytokines including interleukin-1 (IL-1) (Takaesu et al. 2001). TAK can be an upstream kinase of 5 adenosine monophosphate-activated proteins kinase (AMPK), an integral energy sensing kinase involved with heart stroke. We have lately discovered that inhibition of TAK1 is definitely neuroprotective after focal ischemia (White colored et al. 2012). Our earlier work shown that Ivacaftor neuroprotective ramifications of TAK1 inhibition are self-employed of its activation of AMPK (White colored et al. 2012). In today's study, we used GSK-3 Inhibitor VIII, a particular and extremely potent GSK-3 inhibitor to examine the consequences of GSK-3 inhibition on ischemic damage and stroke-induced memory space impairment. Furthermore, we looked into relationships between GSK-3, AMPK, and TAK1 signaling through the use of mixed treatment paradigms and Rog coimmunoprecipitation. Outcomes GSK-3 inhibition considerably decreased infarct size Considerably reduced infarct quantities had been noticed after ischemic heart stroke with both early and postponed inhibition of GSK-3. Immediate treatment having a GSK-3 inhibitor in the starting point of heart stroke led to a substantial decrease in cortical (automobile 51.1 2.8 versus medication 40.1 3.7; 0.05), striatal (vehicle 67.8 1.6 versus medication 54.8 3.4; 0.05), and total hemisphere (49.4 2.6 versus medication 35.9 2.4; 0.05) (= 10/automobile group; = 11/medication group) infarct at 48 h of reperfusion (Fig. 1A,B). Oddly enough, similar protective results had been also noticed when treatment. Ivacaftor
Background Reduced residual urine volume (RUV) is usually connected with higher
Background Reduced residual urine volume (RUV) is usually connected with higher mortality in hemodialysis (HD). with the addition of possibly predictive baseline covariates from the development of RUV to the result of your time: age group, sex, diabetes mellitus, hypertension, diastolic blood circulation A-966492 pressure, diuretics, angiotensin transformation enzyme inhibitors (ACEI), angiotensin receptor blockers, hypovolemia, chronic tubulointerstitial nephropathy, still left ventricular hypertrophy and preliminary hemodialysis feature. A worth of 0.05 was considered the threshold of statistical significance. The Ethics Committee from the Faculty of Medication, School of Kinshasa accepted the implementation of the research. Results Baseline features of the sufferers Nearly all dialysis sufferers were man (68.8%, sex ratio 2.2), using a mean age group of 52.5??12.3?years (Desk?1). The initiation of HD for the whole group corresponded to a median (IQR) approximated glomerular purification rate (eGFR) regarding to MDRD, serum creatinine and plasma urea of 5.0 (3.0C8.0) ml/min/1.73?m2, 12.0 (8.2C17.8) mg/dl and 197 (147C288) mg/dl, respectively. The median beliefs (IQR) of eGFR, serum creatinine and plasma urea of HD sufferers with and without conserved IUV (preliminary urine quantity) had been 6.0 (4.0C8.0) vs 4.0 (3.0C8.0) ml/min/1.73?m2 (systolic blood circulation pressure, diastolic blood circulation pressure, angiotensin transformation enzyme, angiotensin receptor blockers, estimated glomerular purification ratio, adjustment of diet plan in renal disease Sufferers with preserved preliminary RUV, in comparison to those with a short RUV of significantly less than 500?ml/time, were often on diuretics (60% vs 46%, respectively; interquartile range, angiotensin transformation enzyme, angiotensin receptor blockers Elements considerably from the preservation/reduce of urine quantity (ml/time) as time passes As the Desk?3 shows, period includes a significant quadratic influence on RUV. The harmful beta coefficient (for period) implies that the common RUV decreased as time passes. The positive coefficient (for the quadratic term, period2) implies that there is a deceleration of the drop (the drop slowed down as time passes). The usage of ACE inhibitors on the initiation of HD (beta coefficient of 219.5, angiotensin conversion enzyme, standard mistake, Akaike details criterion For the awareness analysis, the model where the quadratic term of your time was allowed being a random impact didn't converge. Nevertheless, the model 2 formulated with an unstructured covariance matrix (AIC?=?6410.8.) supplied a better suit than people that have the exchangeable (AIC?=?6427.3) as well as the AR(1) (AIC?=?6420.4) covariance buildings. Figure?3 displays the marginal ordinary of VUR predicted with the model 2 and better shows the quadratic design of RUV as time passes. Open in another home window Fig. 3 Model 2 forecasted marginal method of residual urine amounts (ml) as time passes with 95% self-confidence intervals (CIs) Debate This research evaluated predictors of RUV preservation in HD sufferers. The mean RUV on the initiation of HD was up to 680?ml. That is considerably elevated set alongside the data reported in the decision research [31]. RUV was also better maintained in today's research than in the decision research. Indeed, twelve months following the initiation of HD, 60% of individuals in this research still yielded ?250?ml/day A-966492 time instead of 23.2% in the decision research [31]. Several comorbidities as well as the more advanced age groups of HD individuals in Traditional western countries could clarify this difference. Whenever we analyze the result of time only, we remember that the rate of the decrease in RUV slowed up as time passes. This impact remained present actually after modifications for ACE inhibitors make use of, chronic tubulointerstitial nephropathy and LVH in model 2. This slowing from the decrease in urine quantity as time passes could be related to a noticable difference in the technique of dialysis using the introduction from the HDF technique in both research centers since 2010. HDF is definitely characterized by a little reduction in RUV, much like peritoneal dialysis. Today's research did not display a link between urine quantity and age group or sex. These email address details are unlike those of the books [4C7]. Nevertheless, Nechita et al., in A-966492 a report of the cohort of 216 individuals with stage 5 chronic kidney disease (CKD) starting chronic HD, also didn't look for a significant association between residual diuresis and age group or sex [32]. It's possible that the tiny sample size didn't reveal the impact old or sex on the increased loss of urine quantity. Another reason could be associated with the actual fact that urine quantity is the consequence of glomerular purification, tubular reabsorption as well as the impact of diuretic therapy. Furthermore, in CKD, the focus and dilution tubular capability is A-966492 altered due to tubulointerstitial fibrosis and improved tubular level of resistance Mouse monoclonal to ITGA5 by ADH [33, 34]. In today's research, self-employed predictors of urine quantity were the usage of ACE inhibitors, chronic tubulointerstitial nephropathy and LVH. The helpful part of ACE inhibitors on RKF and RUV in HD continues to be shown in the evaluation from the U.S. Renal Data Program data, a report of 2211 event dialysis individuals.
Introduction Cartilage oligomeric matrix proteins (COMP) is available in elevated concentrations
Introduction Cartilage oligomeric matrix proteins (COMP) is available in elevated concentrations in sera of individuals with joint illnesses such as arthritis rheumatoid (RA) and osteoarthritis (OA). lupus erythematosus (SLE), weighed against healthy settings. SLE individuals with arthritis experienced considerably higher COMP-C3b amounts than do those without. COMP-C3b was furthermore raised in individuals with ankylosing spondylitis (AS), psoriatic joint disease (PsA), reactive joint disease, systemic sclerosis, and OA. COMP-C3b didn't correlate with COMP in virtually any of the individual organizations. COMP-C3b correlated with disease activity in RA, however, not in additional illnesses. COMP-C3b amounts in RA individuals reduced on treatment with tumor necrosis element (TNF)- inhibitors, whereas the amounts increased in individuals with AS or PsA. The adjustments of COMP-C3b didn't parallel the adjustments of C-reactive proteins (CRP). Conclusions COMP-C3b amounts are elevated in a number of rheumatologic illnesses and correlate with inflammatory steps in RA. COMP-C3b amounts in RA reduce during TNF- inhibition in a different way from those of CRP, recommending that development of COMP-C3b pertains to disease features not really shown by general swelling measures. Introduction Arthritis rheumatoid (RA) can be a disabling disease with both a big impact on the grade of lifestyle for the individual and a higher BI-847325 manufacture economic effect on society. It really is known that early involvement minimizes Mouse monoclonal to CD4.CD4, also known as T4, is a 55 kD single chain transmembrane glycoprotein and belongs to immunoglobulin superfamily. CD4 is found on most thymocytes, a subset of T cells and at low level on monocytes/macrophages injury and disease development. Therefore particular and delicate diagnostics are crucial for early breakthrough of disease. Despite the fact that rheumatoid aspect (RF) and anti-citrullinated peptide antibodies (ACPAs) are trusted as diagnostics for RA, improvements are had a need to improve the specificity and awareness of current molecular markers in RA. Therapy also needs BI-847325 manufacture to improve when brand-new diagnostic assays can differentiate RA sufferers into groupings with different root pathologic mechanisms. Many methods to develop novel serologic markers for RA have already been attempted, one of these being dimension of cartilage BI-847325 manufacture oligomeric matrix proteins (COMP) in serum or synovial liquid. COMP can be a structural element of cartilage, and it's been been shown to be released during erosive joint illnesses such as for example RA and osteoarthritis (OA) [1]. Extreme creation of COMP in your skin in systemic sclerosis (SSc) can be reflected by a rise in serum COMP [2]. COMP can be a homopentamer of 435 kDa where every individual monomer comprises four epidermal development aspect (EGF) domains, eight thrombospondin type 3 (TSP3) domains, and a globular C-terminus. The stores polymerize via their N-terminal coiled coil domains, which can be stabilized by interchain disulfide bonds [3]. One function of COMP in tissues can be to catalyze collagen fibrillogenesis [4,5] and, in the adult, to stabilize tissues structure by getting together with various other collagen-bound matrix protein [6]. Within a prior study, we BI-847325 manufacture demonstrated that COMP released through the joint parts during RA can activate go with both em in vitro /em and em in vivo /em [7]. Complexes between COMP as well as the complement-activation item C3b were discovered both in the serum and synovial liquid of RA sufferers, whereas no COMP-C3b was within the serum of healthful handles or in sufferers with OA; as a result, it was figured COMP-C3b may be diagnostic in RA. The complement-activating site was proven to BI-847325 manufacture reside inside the C-terminal percentage of COMP, by discussion with both properdin and C3. Go with is an essential section of innate immunity, and its own uncontrolled activation continues to be strongly implicated in lots of autoimmune illnesses, included in this, RA. Several research show that go with insufficiency or inhibition ameliorates disease activity in rodent types of RA [8,9], and complement-activation items have been within the joint parts of individuals with RA [10-12]. Many proteins that are located in affected bones have been proven to activate match, included in this RF [13,14], ACPA [15], cartilage substances of the tiny leucine-rich-repeat proteins (SLRP) family members [16,17], aswell as apoptotic cells [18] and therefore most likely donate to disease development by nourishing the inflammatory response. Dimension of.
Healing regulation of tissue vascularization has appeared as a stylish method
Healing regulation of tissue vascularization has appeared as a stylish method of treat several human diseases. is definitely available to certified users. the positioning from the gel. aCf vessels perfused with i.v. printer ink shot and stained with DAB/H2O2, gCi vessels just stained with DAB/H2O2 The perfect nylon mesh was chosen by evaluating mesh opportunities of 100, 300, 600 and 900?m (not shown). The amount of vascular ingrowth was related for those mesh sizes. Nevertheless, the 100?m mesh tended to flex having a nonuniform attachment towards the CAM as well as the 600 and 900?m mesh sizes often injured the CAM because of stiffer nylon threads. Which means 300?m mesh size was determined. Implant buy Pentostatin neovascularization is definitely improved by FGF-2 and PDGF-BB however, not by VEGF-A Implanted fibrin-collagen matrices had been vascularized in 20?% of instances without addition of development elements. FGF-2 or PDGF-BB triggered a 3 to 4 fold upsurge in the amount of vascularized implants. Remarkably, VEGF-A [4] didn't stimulate implant vascularization, buy Pentostatin while TGF experienced a solid inhibitory influence on spontaneous aswell as FGF-2-induced neovascularization (Fig.?3a). The shortcoming of human being VEGF-A to induce neovascularization had not been related to varieties differences once we discovered that hVEGF-A was identified by poultry buy Pentostatin endothelium and induced a considerable upsurge in buy Pentostatin vascular permeability when used on neovessels from the vascularized implant as demonstrated from the TRITC-dextran extravasation assay, which is buy Pentostatin definitely analogous towards the Kilometers permeability assay [40] (Fig.?3b, c and Supplementary video clips 1 and 2). Open up in another windows Fig.?3 Ramifications of growth elements on neovascularization. a rise elements had been examined for their capabilities to activate vascularization of the fibrin-collagen matrix implanted within the CAM and examples scored inside a binomial way (yes or no). FGF-2 (250?ng) and Tnfsf10 PDGF-BB (250?ng) induced vascularization, even though human being VEGF-A (250?ng) had zero impact. TGF- (100?ng) blocked spontaneous and reduced FGF-2-induced vessel ingrowth. b, c hVEGF-A induced TRITC-dextran extravasation from your implant neovasculature. 6?times after implantation 155?kDa TRITC-dextran was injected i.v. and neovessels that grew through the grid had been 1st imaged for 15?min (12?min shown) after software of saline (control) and for yet another 15?min (12 min shown) after activation with hVEGF-A (VEGF). Basal degree of leakage from your neovessels was robustly improved after software of hVEGF-A. Remember that in (b) the VEGF-A impact is definitely underestimated as fluorescence transmission quickly reached the saturation level (observe Supplementary video clips 1 and 2) Inhibition of angiogenesis could be recognized from toxic results on pre-existing vessels An array of substances recognized to inhibit angiogenesis in vivo was examined for their capability to inhibit FGF-2 induced neovascularization of gel implants within the CAM. Both ingrowth of neovessels and possibly toxic ramifications of the medicines on pre-existing vessels in the CAM beneath the implants had been examined (Fig.?4a, b). Vascular ingrowth was obtained on the binomial (yes or no) level inside a double-blinded way. Fumagillin [41] as well as the MEK inhibitor U0126 [42] inhibited neovascularization while thalidomide [43] acquired no influence on vessel development like this of quantification (Fig.?4a). non-e of these chemicals affected pre-existing vessels (Fig.?4b). PD173074, which can be an FGFR1, FGFR3 and a vulnerable VEGFR2 inhibitor, decreased FGF-2-reliant implant vascularization within a dosage dependent way but didn't have an effect on PDGF-dependent vessel development. This experiment demonstrated an inhibitor put into the gel is certainly released as time passes and that dosage response relationships could be set up (Fig.?4c). To help expand characterize discharge kinetics of hydrophobic chemicals.


text:
Sunday, August 29, 2010
Daily Thoughts 8/29/2010 (The Facebook Era, The Oxford English Dictionary )
Daily Thoughts 8/29/2010
I read another chapter in the Facebook Era by Clara Shih. She was writing about social media as a recruiting tool for business. Businesses are looking for candidates through Facebook, Linkedin, Ryze, and other networks. If you are a recruiter who is interested in the social media side of recruiting, there is http://www.ere.net/ Electronic Recruiting Exchange network which has a very nice set of articles on the subject. They also have a national conference every year. I still get invites to it, even though I haven't been in recruiting for many years.
I think I might be working on some bookmarks and a flyer next week for small business books. We are having a five session long program on entrepreneurship in September presented by the Womens Enterprise Development Center, the mayor is doing a small business expo part of it is going to be held at the library, and we have been purchasing quite a bit of material on small business lately. Plus it coincides with my own interests.
I added some more book reviews from August 2009 to my book review list. This is a steady long term project.
The Oxford English Dictionary is moving online only. The last time I used this was to find the etymology of some words for someone. It is not very practical for modern usage. I can understand why it is going online. Still, I think it should be available through print on demand for a considerable fee if someone wants it. http://www.nwcn.com/news/Oxford-English-Dictionary-may-be-going-online-101756313.html
Posted by Book Calendar at 5:44 AM
No comments:
Subscribe to: Post Comments (Atom)


text:
Powered by experts. Published by you.
Operated by Author Solutions
- Google+
Bookstore | CHILDREN'S | FICTION
Liz is a young bison calf who loves life and wants to share her environment with you. When the sun is shining on the beautiful grasslands of Yellowstone Park, she and the rest of the bison herd have a lovely time running, playing, and resting.
Here, Liz shares interesting facts about herself and her bison family. She explains how she will grow larger and her fur will become darker. She will begin to look like her parents. The bison herd always stays near a water source, such as the lakes and rivers of the North American plains. Although they must watch out for predators, they live on protected land in national and state parks, where visitors may see them. With Liz's help, you can learn all about the daily activities of the Yellowstone bison.
This children's book, complete with original photographs, offers an introduction to the life and characteristics of bison and makes young readers aware of the need for conservation.
Call 1-888-242-5904 | Publishing Guide
© 2019 Simon & Schuster, Inc. and Author Solutions, LLC Archway Publishing and related logos are trademarks of Simon & Schuster, LLC All rights reserved.Privacy Policy | Terms of Use | Browser Requirements | Site Map | About Us | Affiliates
E-Commerce Powered by nopCommerce