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The penis ring is an accessory that pleasantly condenses intimate life and is used to increase sexual pleasure. This sex toy tightens the base of the penis and the testicles, and thus the blood pressure in the member increases, which results in increased sensation, greater dilation, and firmer erection.
Penis rings exist in many shapes and colors. Some are simple, while others have accessories that vibrate the clitoris during sexual intercourse. On the other hand, these rings can be installed at any time of the erection.The penis ring should be worn for a maximum of 20 minutes. Men'sHealth helps us how to use cock rings.
The maximum duration of their wearing is 20 minutes, exceeding this time can affect the capillaries or bruise the penis.The use of rings is not recommended for men with vascular problems, diabetes or nerve injuries. If you feel uncomfortable about wearing them, take them out immediately. Too tight rings can cause pianism, which can lead to gangrene of the penis. If you fall asleep while wearing a penis ring, you may have serious problems with permanent nerve injuries.
To use the penis ring, pinch it around the base of the genital organ and back of the testicles. Men can opt for penis rings if they suffer from erectile dysfunction as they will help them maintain their erection. Some men just like sex toys because of the sensation of tightening and the surplus of firmness.
Penis massage can be a great way to excite or even satisfy your partner. Sexologists recommend using the lubricant, for erotic massage to have the desired effect. Here are some techniques for penis massage:
- Hold the penis with both hands and move the palms in opposite directions. Friction will create a feeling of warmth. Be careful not to apply too much pressure. You can alter this movement with one from the top down.
- The head of the penis can be massaged gently with a well-lubricated palm, while the other is the base of the penis. Movements are circular. Be careful to alternate meanings from time to time.
- While your penis is in the usual position on the abdomen, you hold the testicles with one hand and the other one between the body of the penis and the abdomen, so that you can massage it with the palm of your hand, moving from top to bottom.
- A very dynamic massage technique is one in which you hold the penis with one hand, and with the other you slightly rotate the body of the penis, holding the penis's head with the thumb and the index finger.
- This technique involves coordinating both hands, but is easy to put into practice. Grasp the top of the penis with your palm and lower your palm to the base of your penis. Then let go of your penis, making sure you take the head of the pen with your other hand and repeat the motion.
- Of course, the technique above can be done and vice versa. Strange a hand with the base of the penis and move it upwards, then let it go and repeat the movement with the other hand.
- Place both palms on one side of his or her pension, without covering it with your fingers. Next, gently push your palms together to create pressure and move the palms from the top to the bottom.
- Massage the head of your penis as if you were trying to unlock a door that has a round yell. The goal is to lunge with the circular motion around the penis, not to "unlock the door."
- With this slightly more complicated technique, you will give it the impression that stimulation resembles sex. Grasp the penis head slightly into the center of your palm, holding your hand tight in the form of a punch. Move your hand down so you can tighten your penis's head in your fist. As soon as you moved sufficiently down and he was discovered, change your hands to give him the feeling of a continuous penetration.
- Form a thumb and thumb ring and move it from the top to the bottom of your penis. The pleasure comes from the fact that you reduce the circumference of the ring when you climb your hand to the head of the penis and then force it to penetrate the tight space when you move your hand down.
These techniques will cause you real pleasure by combining them. For example, you can massage your penis body until you notice the penis's head swelling and red. Then you can combine the second, eighth, and ninth techniques in that order.
Sometimes a big cock is the one that raises problems in the bedroom. Sometimes it's a too small penis. And the vagina, either too tight or less firm, can be "guilty" of the failure of a sex party. There are solutions, certain sexual positions or other erotic tricks that can alleviate almost any type of mismatch.
Even if the attraction cannot be denied, and sexual chemistry breaks the "thermometers", there are cases in which the genitals seem to not fit Penis too big or too small, a narrow vagina or a less firm, and difficult alignment in certain positions because of the height, is features that can hurt a successful sex match.
The penis too big
Although it does not seem to be a problem, but a gift for most men, the too large size of the penis can put an end to a successful sex party. To avoid discomfort, multiple sexual positions, as well as deep-throat oral sex and passionate sessions should be avoided. To solve this problem, you can use a few special rings that underlie the penis and prevent the man from reaching the cervix of the partner. These are reusable and can be washed with antibacterial soap. For more information, visit Lustplugs.com.
Does the vagina too tight
If it is difficult to have sex or to finish a game because of the partner's too narrow vagina, you can use a simple trick. The woman squeezes on the abdomen with a lubricant, sits on the back, and holds his penis with his palm, like a sandwich, between the hand and the abdomen. For the success of this trick, the position of the missionary is the most appropriate, allowing the hugs and kisses of the action very similar to sex with penetration. Also, in order for the woman to get excited properly before penetration and to relax, she may try to use a vibrator.
Does not have the vagina firm
Kegel exercises are a healthy and simple way to strengthen your pelvic muscles. Sexologists especially recommend Kegel exercises on the toilet as you urinate, but you can do it anywhere, anytime. In this case, you can try sex in the "position of the spoon", which means staying stretched out on one side and penetrating it from behind. Helpful are vaginal balls used during sex.
You do not fit because of the height
Sometimes, either he has feet too short or too long, some positions are not easy to achieve. As usual doggy style is the one that poses problems, using more pillows under the knee is a great solution to address this position.


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Modulation of TNF-α-converting enzyme by the spike protein of SARS-CoV and ACE2 induces TNF-α production and facilitates viral entry
Edited by Diane E. Griffin, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, and approved March 6, 2008 (received for review November 28, 2007)
Severe acute respiratory syndrome coronavirus (SARS-CoV) is a high-risk infectious pathogen. In the proposed model of respiratory failure, SARS-CoV down-regulates its receptor, angiotensin-converting enzyme 2 (ACE2), but the mechanism involved is unknown. We found that the spike protein of SARS-CoV (SARS-S) induced TNF-α-converting enzyme (TACE)-dependent shedding of the ACE2 ectodomain. The modulation of TACE activity by SARS-S depended on the cytoplasmic domain of ACE2, because deletion mutants of ACE2 lacking the carboxyl-terminal region did not induce ACE2 shedding or TNF-α production. In contrast, the spike protein of HNL63-CoV (NL63-S), a CoV that uses ACE2 as a receptor and mainly induces the common cold, caused neither of these cellular responses. Intriguingly, viral infection, judged by real-time RT-PCR analysis of SARS-CoV mRNA expression, was significantly attenuated by deletion of the cytoplasmic tail of ACE2 or knock-down of TACE expression by siRNA. These data suggest that cellular signals triggered by the interaction of SARS-CoV with ACE2 are positively involved in viral entry but lead to tissue damage. These findings may lead to the development of anti-SARS-CoV agents.
Severe acute respiratory syndrome coronavirus (SARS-CoV) is an infectious pathogen known to have caused acute respiratory distress in >8,000 patients with a mortality rate of ≈10% (1). Although outbreaks of SARS-CoV are now well controlled, the mechanism of severe respiratory failure in infected patients is unknown. Angiotensin-converting enzyme 2 (ACE2), an ACE homolog that functions as a positive regulator of the renin-angiotensin system (RAS) (2, 3), was identified as a receptor of SARS-CoV (4). A possible indicator of a severe clinical outcome, the spike protein of SARS-CoV (SARS-S) was found to down-regulate ACE2 expression (5). ACE2 knockout (KO) mice were also shown to be susceptible to severe respiratory failure after chemical challenge (5, 6), and ACE2 has been shown to moderate ACE-induced intracellular inflammation, suggesting that the mechanism of ACE2 down-regulation may explain the molecular basis of SARS-CoV-related severe respiratory distress.
HNL63-CoV, a CoV (7) that causes the common cold, was recently found to use ACE2 for viral infection (8), and it was further shown that the spike protein of HNL63-CoV (NL63-S) binds ACE2 directly (9). NL63-S and SARS-S show 21% identity (10), whereas that between NL63-S and the spike protein of HCoV-229E, which uses CD13 [a completely different carboxy (C)-peptidase] as a cellular receptor (11), is 55%. It is important to note that, despite their phylogenetically distinct properties and their producing different clinical outcomes, SARS-CoV and HNL63-CoV both use ACE2.
Based on these observations, we hypothesized that the functional modulation of ACE2 may be differentially induced by SARS-S and NL63-S. To test this prediction, we first clarified the mechanism of SARS-S-induced ACE2 down-regulation, and then compared the cellular responses induced by each protein. We found that SARS-S induced TNF-α-converting enzyme (TACE)-dependent shedding of the ectodomain of ACE2, and that the process was coupled with TNF-α production (12). Experiments using deletion mutants of ACE2 revealed that the cytoplasmic tail of ACE2 is required for shedding. Furthermore, viral infection with SARS-CoV was significantly decreased in cells expressing mutant versions of ACE2 lacking the cytoplasmic domain. In addition, siRNAs of TACE and ACE2 blocked viral infection. These observations suggest that TACE activity is modulated by SARS-CoV via the cytoplasmic domain of ACE2, and that it facilitates viral entry but also causes tissue damage through TNF-α production. Together with data showing that NL63-S does not evoke these cellular responses, our results indicate that the cellular components involved in SARS-S-induced ACE2 shedding are good targets for anti-SARS-CoV therapeutics.
SARS-S Induces Shedding of the Ectodomain of ACE2.
We first examined the mechanism of ACE2 down-regulation after SARS-S binding. In total cellular extracts prepared from Vero E6 cells infected with a pseudotyped virus expressing S protein (S-virus), ACE2 expression was reduced, whereas the expression of an 80-kDa protein in the supernatants was increased (Fig. 1a, lanes 4 and 5). Interestingly, this 80-kDa peptide was not detected by an antibody raised against the cytoplasmic domain of ACE2 (Fig. 1a, lanes 4 and 5, middle row in Lower). Conversely, a control pseudotyped lentivirus expressing vesicular stomatitis virus G protein (VSV-G) did not induce any change in ACE2 expression (Fig. 1a, lanes 6–10). Moreover, recombinant SARS-S showed the same effects (Fig. 1b, lanes 4–6, arrows), whereas binding-defective mutants of the protein (Fig. 1c, ΔC and ΔN) did not (Fig. 1d, lanes 2–4). Consistent with a report showing that the secreted form of ACE2 is the active form (shed form) (2), C-peptidase activity was detected in the supernatants of cultured cells treated with SARS-S (Fig. 1e). Therefore, we concluded that SARS-S down-regulates ACE2 by inducing shedding of the ACE2 ectodomain.
TACE Is a Critical Cellular Factor in SARS-S-Induced ACE2 Shedding.
It has been shown that TACE and ADAM17, a member of the ADAM (a disintegrin and metalloprotease) family (12, 13), are required for the induction of ACE2 shedding by phorbol esters [phorbol-12-myristate-13-acetate (PMA)] (14). Therefore, we examined the effects of TAPI-0, an inhibitor of TACE (15), on SARS-S-induced ACE2 shedding. As shown in Fig. 2a, TAPI-0 efficiently attenuated C-peptidase activity in the culture supernatant. Western blot analysis also indicated that TAPI-0 inhibited SARS-S-induced ACE2 shedding (Fig. 2b, lanes 5 and 6). To further determine the involvement of TACE, ACE2 shedding was examined by forced expression of TACE cDNA in transformed mouse fibroblasts derived from tace-KO mouse fibroblasts (tace-KO cells) (16). As shown in Fig. 2c, the tace-KO cells exhibited SARS-S-induced ACE2 shedding when both TACE and ACE2 cDNA was introduced (Fig. 2c, lanes 4 and 5). To obtain direct evidence that TACE plays an important role in ACE2 shedding, TACE siRNA was introduced into Huh-7 cells and SARS-S-induced ACE2 shedding was examined. Huh-7 is a human hepatoma cell line permissive for SARS-CoV infection (17, 18). As shown in Fig. 2d, ACE2 shedding by SARS-S was significantly attenuated by TACE siRNA. As shown in Fig. 5c, TACE siRNA successfully reduced endogenous TACE expression.
The ACE2 Cytoplasmic Domain Is Required for SARS-S-Induced ACE2 Shedding.
Cellular TACE was activated by the interaction of SARS-S with ACE2, strongly suggesting the involvement of the cytoplasmic domain of ACE2 in this phenomenon. To confirm this suggestion, we prepared several mutants lacking the C-terminal region of ACE2 (Fig. 3a). After confirming the expression of each mutant protein (Fig. 3b), we examined shedding of the ectodomain by SARS-S. SARS-S-induced ACE2 shedding was not observed with a chimeric mutant in which the transmembrane and cytoplasmic domains of ACE2 were eliminated [transmembrane and cytoplasmic domains (ΔTMC) Fig. 3c]. This mutant was able to bind SARS-S (Fig. 3d), and it was susceptible to ectodomain shedding when the cells were stimulated with PMA (Fig. 3e). Experiments with additional deletion mutants revealed that the last 17 aa of the ACE2 C terminus were dispensable for ACE2 shedding (Fig. 3f). Similar to ACE2 shedding, the production of TNF-α was detected in the culture supernatants of cells expressing WT and ΔC17-ACE2 (Fig. 3g). In contrast, no TNF-α production was observed in cells expressing deletion mutants of the cytoplasmic tail of ACE2 (Fig. 3g, P < 0.01).
The ACE2 Cytoplasmic Domain and TACE Are Positively Involved in Virus Entry.
We investigated the role of the cytoplasmic tail of ACE2 and TACE in viral infection. In a reporter assay based on a pseudotyped lentiviral system, no difference in infection was detected between cells expressing WT and ΔTMC-ACE2 (Fig. 4a). Although this observation is consistent with previous reports, we observed a reproducible difference in viral entry based on the intracellular p24 concentration after infection with the same virus (Fig. 4b). To obtain conclusive evidence, we infected the cells with SARS-CoV and quantified SARS-CoV mRNA by real-time RT-PCR. As shown in Fig. 4c, deletion of the cytoplasmic tail greatly reduced viral infection. The efficiency of infection was also markedly reduced in cells infected with ΔC21 or ΔC32-ACE2 (Fig. 4d), even with ΔC17-ACE2.
We next examined the role of TACE in viral infection, using TACE siRNA. As shown in Fig. 4e, viral entry was significantly attenuated (P < 0.05) by the siRNA, whereas control or ADAM10 (control metalloprotease) siRNA did not decrease the incidence of viral infection. It is important to note that ACE2 siRNA significantly decreased the SARS-CoV mRNA copy number (Fig. 4e, P < 0.01), indicating that infection by SARS-CoV via ACE2 was successfully detected by real-time RT-PCR. As shown in Fig. 5c, each siRNA efficiently blocked expression of the target protein. These observations indicate that the cytoplasmic tail of ACE2 and TACE are positively involved in SARS-S-dependent viral infection. In addition, the level of intracellular p24 after infection with pseudotyped lentivirus is a good indicator of viral entry.
HNL63-CoV Spike Protein Does Not Induce ACE2 Shedding or TACE Activation.
To determine the specificity of the ACE2 shedding and TNF-α production induced by SARS-CoV, we investigated the functions of HNL63-CoV. In marked contrast to SARS-S, NL63-S did not induce ACE2 shedding (Fig. 5a, lane 4), although it was able to use ACE2 as a cellular receptor (Fig. 5d). Furthermore, NL63-S did not induce TNF-α production (Fig. 5b). To characterize the differential mode of infection between SARS-S and NL63-S, we examined the effects of siRNAs against ACE2, TACE, and ADAM10, and we examined viral entry by measuring the level of intracellular p24. We began by confirming that each siRNA or combination of siRNAs successfully interfered with expression of the endogenous gene products (Fig. 5c). As shown in Fig. 5d, the ACE2 and TACE siRNAs efficiently blocked viral entry (P < 0.05 and 0.01, respectively), whereas the ADAM10 siRNA did not. However, we detected no definite effect of TACE siRNA on NL63-S infection. The ACE2 and TACE or ADAM10 siRNAs, however, did significantly inhibit NL63-S viral entry.
Taken together, these observations suggest that ACE2 and TACE are differently required for infection by SARS-S and NL63-S and that the functional role of TACE is more prominent for SARS-S than for NL63-S.
Down-regulation of ACE2 was proposed to be associated with a severe clinical outcome in cases of SARS-CoV infection, but the mechanism remained to be clarified. In the present study, we found that SARS-S induces ACE2 shedding via a process that is tightly coupled with TNF-α production. In contrast, the spike protein of HNL63-CoV, a CoV and etiological pathogen of the common cold that uses ACE2 as its cellular receptor, did not induce such cellular responses. These observations suggest that ACE2 shedding and its causative cellular signals are attributable to SARS-CoV-induced tissue damage.
As shown in Fig. 3g, the induction of TNF-α production by SARS-S depends on the cytoplasmic tail of ACE2 and TACE activity. Because treatment with SARS-S did not increase the expression of the membrane-bound precursor of TNF-α [supporting information (SI) Fig. S1], it seems that TACE activity is directly affected by the attachment of SARS-S to ACE2. Lambert et al. (14) proposed that PMA-driven ACE2 shedding solely depended on TACE. In addition, the results of our experiments using siRNAs against TACE and ADAM10 revealed that SARS-S-induced ACE2 shedding depends on TACE activity. Interestingly, however, the forced expression of ADAM9 or 10 rendered tace-KO cells competent for SARS-S-dependent viral infection (Fig. S2), implying that TACE may be complemented by the overexpression of other ADAMs. We also observed that rottlerin, a PKC inhibitor (19), partially blocked the phenomenon (Fig. S3). It is likely that functional modulation of the cytoplasmic tail of ACE2 triggers multiple cellular signals involving PKC activation.
Intriguingly, we found that the entry of SARS-CoV depends on the cytoplasmic tail of ACE2 and TACE activity, which was inconsistent with previous reports indicating that the cytoplasmic tail of ACE2 is dispensable for viral infection (18, 20). However, these previous studies were performed by using pseudotyped lentiviruses. As shown in Fig. 4a, we also detected no differences in reporter gene expression in cells expressing WT-ACE2 and ΔTMC-ACE2. However, viral entry, as judged by the amount of p24, a component of the HIV-1-based lentivirus (Fig. 4b), was significantly higher in cells expressing WT-ACE2 than in those expressing ΔTMC-ACE2. Because the replication of SARS-CoV does not require viral integration into the host genome, we assumed that a reporter assay based on a pseudotyped lentivirus was not relevant for evaluating the roles of the factors involved in SARS-CoV infection. Given these expectations, we measured viral entry based on real-time RT-PCR detection of SARS-CoV mRNA. As shown in Fig. 4c, we successfully detected a difference in copy number of the viral mRNA in these cells (P < 0.01). The introduction of an ACE2 siRNA also reduced the copy number of SARS-CoV mRNA (Fig. 4e), indicating that this method actually detects viral infection via ACE2. In addition, TACE siRNA reduced SARS-CoV infection, indicating that TACE plays an important role in the entry of SARS-CoV. Consistent with these data, TAPI-0, a TACE inhibitor, specifically inhibited the entry of SARS-S (Fig. S4). In addition, although ΔTMC-ACE2 was defective in terms of viral infection, it restored receptor function when TACE was activated by addition of PMA (Fig. S5).
It is unclear how TACE facilitates viral entry. Our observations indicated that the TACE requirement is stronger for SARS-S than for NL63-S (Fig. 5d). Recent studies, using peptidases and their inhibitors, suggested that SARS-S is processed in a stepwise fashion (21, 22). Peptidase inhibitors of cathepsin L (23) or Ben·HCl (24) blocked viral infection, whereas the addition of cathepsin L or Factor Xa enhanced viral infection (23⇓–25). The peptidase activity of cathepsin L, which is critical for the fusion of viral particles and cytoplasmic membranes, depends on a low endosomal pH (26). Interestingly, SARS-CoV depends on both cathepsin L (25) and a low endosomal pH (26), whereas HNL63-CoV (25) does not, indicating a possible functional link between TACE and the components required for fusion of viral and plasma membranes.
Because the shed form of ACE2 is catalytically active (Figs. 1⇑–3) and would counteract ACE-induced cellular signals, it is debatable whether ACE2 shedding by itself is a key event leading to tissue damage. It is plausible that prolonged low-level expression of ACE2 is required for the up-regulation of ACE activity in tissue injury. Although it is important to characterize the temporal changes in ACE2 expression after shedding, the results of the present study suggest that the cytoplasmic tail of ACE2 and a cellular response triggered by the interaction between ACE2 and SARS-S are candidate targets for anti-SARS-CoV therapeutics.
Materials and Methods
Cell Culture and Chemicals.
Vero E6, HEK293T, and Huh-7 cells were obtained from the Riken BRC Cell Bank. These cells and NIH 3T3 cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% FCS. tace-KO cells (16) were provided by R. A. Black (Amgen) and maintained in Ham's F-12 supplemented with 2% FCS. Phorbol-12-myristate-13-acetate (PMA) (Sigma) was used as a positive control for ACE2 shedding.
Plasmid Construction.
Plasmids encoding full-length hACE2 (4) and residues 12–672 (27) of SARS-S were provided by H. Choe (Harvard Medical School, Boston, MA). Plasmid DNAs encoding the ACE2 mutants or part of SARS-S were constructed by using PCR-based techniques. Each mutant was confirmed by sequencing.
Preparation and Infection of SARS-S and NL63-S.
Pseudotyped lentiviruses were prepared as described in ref. 28. HEK293T cells were cotransfected with HIV-1-based lentiviral DNA and plasmids encoding full-length SARS-S (G. J. Nabel, National Institutes of Health, Bethesda, MA) (26), NL63-S (S. Pöhlmann, Friedrich Alexander University of Erlangen–Nuremberg, Erlangen, Germany) (8, 9), or VSV-G (29). p24 of gag protein was measured by using a Retro-Tek HIV-1 p24 ELISA Kit (ZeptoMetrix) in accordance with the manufacturer's instructions. ACE2-expressing HEK293T cells were infected with 100 ng/ml p24 in SARS-S. After 4 h (before integration of the lentiviral DNA into the host genome) (C. N.-M., unpublished data), the cells were treated with trypsin-EDTA to degrade all unincorporated virus.
siRNA and Protein Analyses.
siRNAs against ACE2, ADAM10, and TACE were purchased from Ambion and transfected by using Oligofectamine Reagent (Invitrogen) in accordance with the manufacturer's instructions. The sequences are summarized in Fig. S7. The cells were assayed 48 h after transfection.
Monoclonal antibodies against the ACE2 ectodomain (R&D Systems) and polyclonal antibodies against the cytoplasmic tail of TACE (Chemicon), ADAM9 (Santa Cruz Biotechnology), ADAM10 (Chemicon), and β-tubulin (loading control) (NeoMarker) were used for Western blot analysis. Rabbit polyclonal antibodies against the ACE2 cytoplasmic domain were produced by MBL Corp. To examine ACE2 shedding, cells were treated with SARS-S, and the culture supernatants were precipitated with trichloroacetic acid (TCA). As a control, 10 μg of ovalbumin (OVA) was added to each sample and the recovered precipitates were probed with anti-OVA antibodies (Chemicon).
Real-Time PCR Analysis of SARS-CoV mRNA Expression.
HEK293T cells or Huh-7 cells in 24-well plates were infected with SARS-CoV FFM-1 strain at an MOI of 1. Four hours after infection, total RNA was purified by ISOGEN (NIPPONGENE). For quantification of SARS-CoV RNA, real time RT-PCR was performed. As a loading control for normalization, 18S ribosomal RNA was quantified. Primers and probes used in the analysis were summarized in Fig. S6. For all of quantification, a one-step RT-PCR kit (Applied Biosystems) was used and the fluorescence intensity generated from the probe was detected by ABI-7700 sequence detector system (Applied Biosystems).
ACE2 Activity Assay.
The enzymatic activity of ACE2 was assayed by using 10 μM 7-methoxycoumarin-YVADAPK (2,4-dinitrophenyl)-OH (R&D Systems) as a fluorogenic substrate. Fluorescence was monitored at an excitation wavelength of 320 nm and an emission wavelength of 450 nm.
FACS Analysis.
SARS-S was expressed as a chimeric protein with IgG-Fc, using pAB61 (S. Pöhlmann). 293FS cells (Invitrogen) were used for protein expression. The recombinant protein was purified by using G-agarose beads (GE Healthcare). Cells expressing WT-ACE2 or ΔTMC-ACE2 were incubated with the Fc-SARS-S protein, and the bound SARS-S was detected by using FITC-labeled α-IgG.
Solid-Phase Assay to Detect the Binding of SARS-S and ACE2.
Recombinant SARS-S and glyceraldehyde dehydrogenase (GAPDH) were expressed and purified by using a pET vector system (Novagen). ACE2 was partially purified from the supernatants of cultured ACE2-expressing 293FS cells by DEAE column chromatography. ELISA plates (Nunc) were coated with viral mutants, and protein and ACE2 was reacted. After incubation α-ACE2 labeled with Alexa Fluor 555 (Molecular Probes), the bound ACE2 was detected by measuring the fluorescence, using Safire 2 (Tecan).
TNF-α Production Assay.
TNF-α production was measured by using a human TNF-α ELISA Kit (R&D Systems) in accordance with the manufacturer's instructions.
Statistical Analysis.
Statistical significance was evaluated by using Student's t test based on triplicate samples unless otherwise stated.
We thank Drs. R. A. Black, H. Choe, G. J. Nabel, S. Pöhlmann, E. W. Raines (University of Washington, Seattle), A. Zolkiewska (University of Kansas, Lawrence), and R. Postina (Johannes Gutenberg University, Mainz, Germany) for providing the tace-KO cells and plasmid DNAs encoding ACE2, SARS-S, NL63-S/pAB61, mouse TACE, mouse ADAM9, and bovine ADAM10, respectively. We thank Mr. Y. Okudaira and Ms. S. Nakano for technical assistance. This work was supported by Grants-in-Aid for Research on Emerging and Re-Emerging Infectious Diseases from the Ministry of Health, Labor, and Welfare of Japan and the National Institute of Biomedical Innovation.
- ↵**To whom correspondence should be addressed. E-mail: zakay{at}ri.imcj.go.jp
Author contributions: T. Sasazuki and Y.I. designed research; S.H., Norio Yamamoto, C.N.-M., Y.O., and Y.I. performed research; K.T., T. Sata, Naoki Yamamoto, and Y.I. contributed new reagents/analytic tools; S.H. and Y.I. analyzed data; and S.H. and Y.I. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/cgi/content/full/0711241105/DCSupplemental.
- Received November 28, 2007.
- © 2008 by The National Academy of Sciences of the USA
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subset: commoncrawl


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This manicure set keeps nails neat, glossy and strong. It smooths away ridges and discolouration, and gently buffs nails to an exquisite shine within seconds – enhancing their natural colour and promoting stronger growth.
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1. Admission
2. Communication
3. Student employment
4. Student Information System
5. Grade reporting
Registrar's Office is the central administrative unit working in co-operation with faculties and departments to prepare student data, and submit this data to Ministry of Higher Education and Scientific Research.
Students will be accepted according to the rules below:
Knowledge University accepts students according to the rules affirmed by Ministry of Higher Education and Scientific Research. If any changes in the rules of the Ministry, the new rules are valid.
During the application, students fill in a form and can select three of the departments on this form. The students will be placed according to their selection order.
Students who are registered to another university have to withdraw their application form that university in order to register Knowledge University.
The students who fill up the application form must have the following conditions:
The students must have a high school diploma which is approved by the General Directorate of Education.
The international students must have certificate of equality/accreditation from the Ministry of Education.
Students working in governmental offices are not accepted if they don't provide permission letter from their offices.
The application forms have to be approved by high schools and General Directorate of Education.


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Women's Fall Retreat: October 12-13
Needing a boost spiritually, to connect with other women, to laugh and have fun, or a break from the urgency of life's responsibilities? Then you need to come to the 2018 Fellowship Women's Retreat!
This year, we will explore the theme of Daughter of God: Knowing God and Abiding with Jesus
Fueling & Fortifying Sessions:
- Knowing and Being Known by God – Sandi Francis
- Finding Your Place as a Daughter of God – Sandi Francis
- Abiding with Jesus Leads to Great Boldness for God – Laura Madeira
- Abiding with Jesus Leads to Great Thoughts of God – Melissa Ferrari
- Abiding with Jesus leads to Great Contentment with God – Karen Morgan
Price: $130.00 per person, double occupancy
Includes: 6 delicious meals, comfortable hotel-like accommodations with private bath, heated indoor pool, hiking trails, game room, 24 hour beverage bar, a Gift Basket Bonanza raffle, and a 2018 Retreat T-shirt!


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Eubank Jr vs Yildirim Preview With Frank Lotierzo — This Saturday night in Stuttgart, Germany, the World Boxing Super Series super middleweight tournament's second match-up takes place as Chris Eubank Jr faces Avni Yildirim in quarter-final action.
Eubank Jr, the #3 seed in the WBSS' eight man field, will also be defending his minor IBO world title at 168 pounds against the relative unknown in Yildirim. The 168 pound quarter finals kicked off on September 16th when Callum Smith defeated Erik Skoglund via unanimous decision and the tournament continues with George Groves taking on Jamie Cox on October 14th followed by Juergen Braehmer vs Rob Brant on October 27th. As we enter the fight week, the sports books have labelled Eubank Jr the strong favorite despite the fact that the fight is in Germany, familiar confines for Yildirim.
In this latest TBC segment our killer analyst Frank Lotierzo joins us to talk about this weekend's super middleweight match-up between Chris Eubank Jr and Avni Yildirim.
To comment on this video at THE FIGHT FORUM, click here.
Eubank Jr vs Yildirim Preview With Frank Lotierzo / Check out more boxing news and features at The Sweet Science where the best boxing writers write.
Chris Eubank Jr with father and manager Chris Eubank Sr (SKY Sports)


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FileChute 2.5 full review
Emailing large files to clients can be a bit of a lottery. Packets can go missing, mail server inboxes can be filled and many ISPs impose limits on the file sizes you can send. If you're using your Mac for multimedia, graphic design, music or video production, and you use the internet to deliver your work, FileChute 2.5 could make that process much, much easier.
The same applies if you regularly need to back up files on your machine, but keep track of where they're located. You can use it to quickly upload media files and even websites to your webspace too.
FileChute is file transfer tool with a difference. When you start it up it configures itself to connect to your iDisk using WebDAV. If you accept the defaults, you can drag and drop files to your .mac space straight away. FileChute uploads files to a specific folder on your iDisk, archiving them in ZIP format if you wish. It then gives you a URL for the uploaded file that you can paste directly into an email.
Of course, you could do all this yourself. You could create a new folder on your iDisk, archive the files you wanted to upload, drag them to the window and then manually type out the URL in an email instead, but FileChute 2.5 does it all much faster.
There are additional features that make it worth a look at. The tool understands FTP and can connect to other WebDAV compatible servers. You just have to configure them manually with the address, username and password. You can set up as many accounts as you like with different profile names. There's no limit to the file sizes you can transfer.


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For Christmas this year I bought Ms Lee a wonderful spa package so she could indulge in being properly pampered and massaged. For myself, I humbly asked her if I could indulge in my ever growing fetish for pretty lingerie and buy myself a nice babydoll nightie. I told her I would never be so bold as to ask for anything so naughty as being allowed to touch myself or masturbate, but I desperately hoped that once a year she might allow me to pursue my own sexual pleasure in an acceptable manner. I was thrilled when she agreed to let me purchase a nightie. I presented some choices to her and interestingly enough she selected the purple babydoll that was my favorite among the selections. Ms Lee also surprised me by allowing me to not only wear my babydoll nightie on Christmas Eve and day, but she also gave me permission to enjoy all the pleasurable releases I desired on Christmas. This was an incredibly generous gift from Ms Lee since she very rarely allows me such sexual freedom. I frequently go multiple months without being allowed a release at all and when it does suite her whim to allow me a release I am usually given a small window of time to enjoy it. And even when she does allow me a release it might amuse her to insist that it be a ruined release so that my sexual pleasure is minimized. This might seem harsh, but I have been trained to treasure the rare releases she allows me, in whatever manner pleases Ms Lee most. The strict regime of chastity I endure has bound me quite closely to her, something we both appreciate, and I am thankful that it pleases Me Lee to keep me as her property. Ms Lee also instructed me that I am to always wear my new babydoll nightie whenever she allows me a release. That will certainly insure that I will not feel very manly on those rare occasions when I enjoy a release.
I have included a photo of me wearing my new babydoll nightie. I am embarrassed to admit that I took the time and effort to learn how to properly iron my babydoll since it was wrinkled when it arrived. I have added a link on How to properly iron lingerie to the Service Skills section of my blog since this is a skill that I will need to master.
I am quite excited about the new year and I am looking forward to working extra hard for the pleasure of Ms Lee. She has been very generous and patient with me and I desperately want to demonstrate my appreciation. I am also quite excited about the lifelong education program I have embarked upon, with the goal of becoming the best possible service submissive devoted to Ms Lee's pleasure.


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We had heard from others that this was a nice spot, and we were glad to find it was even better than expected. You would think that by now, anchorage off a nice beach would be pretty ho-hum. But really, it is not. Though French Polynesia is beautiful, it really is lacking in nice sand beaches. Many that look sandy in the pictures really are crushed coral and not so nice to walk on.
But, my was it shallow in the anchor spot we had from our friends on s/v Nakia! But Nakia has a slightly deeper draft than we do, so we knew that if they got in there, we could too. We had to stall a bit out in deep water until we got full sun to see our way in. We could tell visually that the water went from 'very deep' (over 100 feet) to 'very shallow sand' (under 10 feet) in the space of a boat length.
As an aside, our depth sounder has been acting wonky lately--we're still trying to figure out what happened to it. It reads OK in depths from 10 feet to 90 feet, but gets messed up in shallower or deeper water. We can live without the deeper water readings, but not having an accurate sounding in shallow water is nerve-wracking.
So we edged slowly up to the sand bank, with Dave on the bow with his polarized sunglasses on, and me in cockpit reading off depths. Once we got on the bank, the depth sounder was flashing again (grrr!). But occasionally it would stop flashing and display around 8 feet. So we were on, not aground, and it looked like the sand was about the same depth around us. So we threw the anchor out in the lee of the little motu, just off the nice sandy beach, and I jumped in to sound the water around us. Feet on the bottom, hand in the air--if my hand is under water, it is over 7' deep. Fins outstretched adds another 18 inches. So we were in 8-10' of water--plenty of depth for Soggy Paws' 5.5 foot draft.
Ahhh!! Beautiful! Unfortunately, this spot is on the charter boat circuit, so later in the day, two other boats came in and dropped anchor near us. But we knew they would only stay overnight. Though we only planned to stay a day there, we ended up staying two. That meant we would have skip a spot on the west coast of Raiatea for the next anchorage (to stay on the rough schedule we had worked out). But it was worth it just to rest for a day and catch up on laundry and small chores.
The wind was still blowing in the 20 knot range, and forecast to continue to do so for a couple more days. But west of Motu Nao Nao, all was tranquil. We were out of the wind, out of the chop, and anchored in deep sand. We felt fortunate that we were in such a comfortable spot, when we heard our friends on the radio talking about the conditions in their anchorages.
The snorkeling on the coral heads out behind the boat, and ahead to the right, was pretty darned good for this part of French Polynesia--the best we have seen since we left the Tuamotus.
At 7/20/2011 6:29 AM (utc) our position was 16°55.15'S 151°25.91'W


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EASY-IMP is a FP7 European research project aiming to develop methodologies, tools and platforms for the design and production of personalised meta-products (product/services), combining wearable sensors embedded into garment with mobile and cloud computing.
The EASY infrastructure will enable collaborative design, production planning and supply chain management for this new range of combined product-service offers based on intelligent wearables.
The EASY business model will introduce immense business scalability by offering to all interested third parties the tools to develop new services for mobile device users, thus evolving into an open platform of literally infinite applications in various target markets.
More specifically EASY will focus on the following pilot applications:
- rehabilitation and developmental games
- personal training
- cardiac rehabilitation


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'He cost me my family's future', says victim of Norfolk fraudster Ben Staff
A victim of policeman turned conman Ben Staff today describes how the fraudster cost him his family's financial future.
It comes as the 35-year-old former Norfolk police officer begins a four and a half year prison sentence after carrying out a series of frauds across Norfolk's building trade which totalled £1m.
Staff gave creditors cheques that bounced and fraudulently claimed VAT refunds, Norwich Crown Court heard on Tuesday.
He was convicted of eight fraud-related charges dating from 2009 to 2014 at two previous trials.
One of his victims, Alex Sexton, said his life had been ruined after he invested in one of Staff's firms.
Mr Sexton was a business partner of Staff and together they built three homes on Waterloo Road in Hainford in 2011.
Mr Sexton said Staff gained his trust when he built the homes on his land.
He then gave Staff £140,000 over several months to invest in a company called Morpheus Homes.
But when he first gave him money in 2012, Morpheus Homes did not exist and he never saw his savings again.
Staff was convicted of fraud and converting criminal property from his dealings with Mr Sexton.
The 67-year-old retiree said the fraud had cost his children and grandchildren their inheritance.
"Before, I was a confident person," he told Norwich Crown Court on Tuesday. "Having been taken in by Ben Staff, I have lost that confidence and I'm very distrustful of people."
He added: "Ben Staff has taken from me my retirement future and children's and grandchildren's inheritance.
"He has a nice house, car and goes on holiday while I have nothing."
He said without support from those around him he would have turned to alcohol.
"He has ruined my life and the future of my family," he added.
Staff's victims included suppliers and contractors in Norfolk's building trade, a business partner, as well as the Inland Revenue.
Jailing him, Judge Mark Dennis told Staff: "You were dealing with honest and hardworking individuals who were acting in the same difficult financial climate that you faced."
He said Staff, of Lucas Court, Thorpe St Andrew, had "continually fobbed people off". "This was thoroughly dishonest conduct," he said. "You slipped into a selfish habit of dishonesty which became your default position."
His frauds first came to light following an investigation by this newspaper in 2013.
Payments relating to his building and development companies went into Staff's own personal accounts.
Police said some of that money was used to fund a holiday, home renovations, pay for his wedding and lease cars.
Staff's accounts showed £5,000 was spent on a holiday shortly after a VAT repayment was made.
The 35-year-old traded off the reputation of the police to gain the trust of victims and then used money intended for business accounts to fund his own lavish lifestyle, Norfolk police said.
But Amiot Vollenweider, mitigating for Staff, disputed that he had used the money to fund his own lifestyle.
He also claimed the total value of the fraud offences was far less than £1m and criticised the press for the way Staff had been portrayed.
Mr Vollenweider told the court Staff had started his businesses after leaving the police in 2008 with honest intentions and had "borrowed from Peter to pay Paul" which had led to the frauds.
He said Staff had not tried to defraud people but had been ambitious and over-stretched his finances which had meant he could not pay all his creditors.
"If it had worked out he would have been a rich man, certainly rich enough to ensure everyone got paid," said Mr Vollenweider.
And he told the judge that as a former police officer being in an "overcrowded prison" would have a "profound effect" on Staff.
But Judge Dennis replied: "A former policeman should have known better."
Speaking after the court case, Det Insp Liz Fernandes, who led the investigation, said: "It gives closure for the victims that the matter is finally resolved and we can move towards confiscation and trying to compensate something for the victims."
Staff still has assets, including three properties, and the court heard a Proceeds of Crime Act hearing was likely to take place in May this year.
Staff was also banned from being a director of companies for 10 years.
His wife, Catriona, 33, was charged in both trials but cleared on all counts.
She was a serving Norfolk police officer during the case and faced internal disciplinary procedures after being suspended from duty in 2014 pending the outcome of legal proceedings.
Staff gestured to family members in court that he would call them as he was led down to the cells
Victims' lives disrupted
The court also heard from two other victims still owed thousands of pounds after carrying out work for Staff.
Martyn Green is owed around £25,000 by Staff for work done to build the houses in Hainford. David Wilson, prosecuting, said: "He was always promised by Staff money would be transferred, only to find he had been let down again." Speaking after the case, Mr Green said: "I see it as closure but at the same time I don't feel it [the prison sentence length] was enough."
Another victim, Gary Lambert, is also owed £25,000, for work he did at Hainford as well as at Frans Green Industrial Estate near Hockering, where Staff was due to build a recycling plant.
"He was in the second year of trading and had to risk his family home to secure his overdraft. It disrupted his life," said Mr Wilson. "He put all he had worked for at risk."
•Do you have a story which needs investigating? Email firstname.lastname@example.com
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The Importance of nutrition in women's health
1. How can working women include nutrition in daily foods. When are supplements necessary?
Women needs more nutrients than men. The different nutritional needs is due to the male and female hormones.
Many working women face iron deficiency because of the blood lose during menstruation. Also it is difficult for them to have a proper meal during the work time. So it is important include meat, fish and poultry in the regular diet. Vegetarians can consume Spinach, kidney beans, black beans, lentils, split peas. These are high in iron source.
Deficiency of Iron or vitamins show particular symptoms or there will be changes in your body. So women have to be very careful in observing the changes in the body. Instead of neglecting these, understand why it is happening. For example iron deficiency may lead to anaemia, tiredness etc. Deficiency of Vitamin B 12 may lead to tingling effect in hands and feet, irritability or depression. This is the time you have to consult your doctor and take the supplements.
But these supplements are useful in safeguarding you against occasional nutrients shortfall. They can't compensate for an unbalanced or unhealthy diet. To make sure that you get all the nutrition, you have to include fruit, vegetables, quality protein, healthy fats in your food.
2. Food habits in working women (example how much should be their water intake, dairy products for calcium, should they avoid too much salt and sugar in their diet…if so then why? What should be their ideal dinner time. What should they eat for breakfast and dinner)
Every person needs minimum 2 litres of water everyday.
Having diet rich food is not sufficient. Also having the food at the right time matters a lot. Skipping breakfast or lunch is not a good idea.
For adults ideal time for dinner should be 7 pm. But working women may find it difficult. So they can have light foods like salads or soups for dinner. They can opt for heavy breakfast in the morning.
Food with too much salt or sugar should be avoided to prevent risk of hypertension and diabetes.
It is better to include 2 servings of fruits in a day. But it is not advisable to eat both fruits together.
Its is good to have 3 big meals and 5 small meals for healthy metabolism and maintaining weight.
3. Vegetarian working women (how can they make up for protein, calcium, omega 3) Do they need to take supplements to make up for the loss.
Vegetarian diets can be healthy. But having a balanced diet requires some extra attention. By eating a variety of foods including fruits, vegetables, legumes, nuts and seeds, soy products, and whole grains, vegetarians can get adequate nutrients from non–meat sources.
- Vegetarians should be sure to eat a variety of whole grains such as whole wheat bread, pasta and tortillas, brown rice, oats, bulgur, and quinoa.
- For the healthy body, fats are also required. Healthy fats include nuts or nut butters, oils, and avocados
- Nuts, nut butters, soy foods, beans, peas, dairy foods such as milk, yogurt, and cheese and eggs all provide good protein.
- Include figs and apricots in your breakfast and snacks to avoid iron deficiency. Green leaves like spinach also helps for this.
4. Nutrition during pregnancy and after child birth (kind of food women should eat before and after child birth)
Your body has increased nutritional needs during pregnancy. Most pregnant women can meet these increased nutritional needs by choosing a diet that includes a variety of healthy foods. Grains are a good source of energy. Fruits and vegetables are packed with antioxidants, fiber, and water-soluble and fat-soluble vitamins. The food groups that include meats, nuts, and legumes provide your body with protein, folate, and iron. Dairy products are the best source of calcium and vitamin D.
5. How long should be the maternity leave for women?
Minimum 3 months of leave should be there. Ideally one can take 6 months leave for a complete recovery. This is mainly for the baby. Baby needs the mother most in these 6 months. Also if the mother stops breastfeeding after 3 months, baby may have infections like ear infections because of the low immunity level.
6. Should women work during the first trimester? When should they get back to work? Is taking a short maternity leave and getting back to work soon after delivery ok for the health of mothers?
Of course yes. She can work in the first trimester unless she has low lying placenta and has heavy bleeding. If she starts working she gets good exercise too.


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1 Bottle Gift Bag - Plain Red
Read more reviews
"Very good would recommend to other business users"
Key Features
- Fits up to a standard bottle of Champagne
- Made from strong 200gsm paper with a contemporary matt finish
- Strengthened handle and base
- Supplied with matching gift tag
- 100% recyclable
Product Overview
Plain red single bottle wine gift bag with all year round appeal. Sold in handy packs of 12 these wine bottle bags make ideal impulse purchases for customers. Make sure they are on display and not hidden away gathering dust under the counter. The profit on a bottle bag can be as much as the profit on the wine bottle itself so it makes sense as a useful source of extra income. The bags come with cotton cord handles, strengthened top and bottom and with a matching gift tag.


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Appreciate this Jason, but it isn't showing any results at all. With the original code, I realized today that some events that are in the admin site in 2015 are not showing up on the live site and some are. I verified that the dates are entered properly (comparing the ones that are showing up to the ones that are not) and they are correct.
Any thoughts would be appreciated.
See PM


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|>>Click here to buy
ProGear Dual Action 360 Multi
Direction 36" Stride Air Walker LS
at a low price online<<
Unlike traditional Air Walkers, the ProGear Dual Action 360 is uniquely designed to provide side-to-side leg stretches as well as forward-backward motion for increased flexibility.
The ProGear Dual Action 360 Air Walker LS offers an exceptional 36" stride length and 180 degree range of motion for a unique and continuous multi-directional motion that provides many different leg exercises, including workouts for your hamstrings, and inner and outer thighs and other leg muscles as well as groin muscles.
|ProGear Air Walker's LCD display
& dual-action handlebars with
pulse grip heart-rate sensors
The ProGear Dual Action 360 Stride Air Walker LS features pulse grip heart-rate monitors in the static handlebars and comes with a simple-to-use LCD computer with a large easy-to-read display that shows motivating workout stats as you exercise, including time, distance, step count, calories burned and pulse. The computer is operated via 2x AAA batteries (included).
The ProGear Dual Action 360 Stride Air Walker LS supports your body weight, providing an effective low-impact workout that's kinder to your knees, ankles and joints than many other kinds of exercise. You can work out at your own pace, simulating a slow walk or a full run. The Air Walker operates super quietly so you can watch TV or listen to music as you exercise without having to turn the sound up!
|ProGear Air Walker's
folding frame
The ProGear Dual Action 360 Multi Direction 36" Stride Air Walker LS is constructed with a 2.75" durable powder-coated tubular steel frame and folds to a nice compact size (1/2 its set-up size) for easy storage with transportation wheels included for easy portability.
The ProGear Dual Action 360 Stride Air Walker LS is suitable for a range of different body shapes/ heights, for people from 4ft 10" tall up to 6ft tall with a user weight capacity of up to 225 lbs.
>>Click here to buy the ProGear Dual Action 360 Multi Direction 36" Stride Air Walker LS with Pulse, at a competitive low price with FREE shipping online<<
Let's take a look at the features and specifications in detail of the ProGear Dual Action 360 to help you decide if it's the best Air Walker for you:
Features of the ProGear Dual Action 360 Multi Direction 36" Stride Air Walker LS with Pulse:
· Suitable for user heights: from 4ft 10" up to 6ft tall
· User weight capacity: up to 225 lbs
· Low-impact total-body workout
· Simulate slow walking or full run or multiple leg exercises
· 36" stride length
· Individual 180 degree range of motion leg exercises
· Forward/backward & side-to-side leg motion
· Work leg muscles, hamstrings, inner & outer thighs, plus groin muscles
· Dual-action handlebars – static & moving arms to work upper body
· Pulse grip heart-rate monitors in static handlebars
· LCD computer with large easy-to-read display
· Displays workout stats: time, distance, step count, calories burned & pulse
· Foot pedals: oversized 15" long with ribbed foot pads
· Handlebars: Foam covered for comfort and firm grip
· Stability: adjustable rear floor stabilizers
· Portability: transportation wheels
· Folding frame: folds to ½ its size for storage
· Folded size: approx. 17" long x 20" wide x 52" high
· Overall dimensions: approx. 34" long x 20" wide x 52" high
· Frame construction: 2.75" tubular steel (powder-coated)
· Weight of machine: approx. 45 lbs
· Power: manual (2x AAA batteries included for LCD display)
· Warranty: 1 year limited
>>Click here to buy the ProGear Dual Action 360 Multi Direction 36" Stride Air Walker LS with Pulse, at a competitive low price with FREE shipping online<<
See what other customers who have bought the ProGear Dual Action 360 Multi Direction 36" Stride Air Walker LS with Pulse have to say about it:
"Me and my family love the ProGear Dual Action Air Walker. I think it's definitely worth the money. Do 5 minutes several times a day or go at it hard core and dripping sweat every time. You will feel a difference using this."
"This ProGear air walker was easy to set up and gives an excellent workout. It's easy on your knees yet burns those calories. Unlike a bike, Fitbit records your steps with this. This helps me to meet my 5 miles a day goal."
"The ProGear Air Walker LS is one of the best purchases I've ever made. It glides smoothly, the monitor is easy to read and understand. It arrived almost completely assembled and doesn't take up much space. Awesome!"
>>Click here to buy the ProGear Dual Action 360 Multi Direction 36" Stride Air Walker LS with Pulse, at a competitive low price with FREE shipping online<<
N.B. It's always advisable to consult your doctor or health care professional before undertaking any new exercise regime.
*Prices/discounts indicated correct at time of writing/publishing. E&OE.
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Information Marketing Niches
The first step for anyone who wants to sell info products is to decide which niche to target. To make the right selection, you need to think BOTH about what will work financially AND what will keep you interested for the "long haul."
At the Fred Info Bootcamp that just ended, there were some interesting examples that you'll find valuable to study and understand.
Joanna Faulk is an educator. She trains both other educators and administrators. At the bootcamp she concluded that the bulk of her audience, the teachers, weren't great prospects for HIGH priced products.
She concluded that she should be producing a wide variety of lower priced products that ALL teachers can afford.
True for your audience? Perhaps. Think about it.
Also at the bootcamp was a guy named Bill Dewees. Bill helps people who want to get into the voice-over business. In about 4 years he has built a business for himself where he makes well over $100k each year.
Bill wants to help other aspiring voice over folks to get into the business and NOT make the same mistakes he did.
Bill WILL have some lower priced products, but you'll also be able to give Bill a LARGE CHUNK OF CHANGE to get personal, one on one coaching from him.
In his case, Bill is devising a series of three high priced "events" that he'll make available to folks who want to get into that business.
Follow this example, because you may be able to use a similar approach in YOUR field.
Bill (with my help) decided to come up with 3 possible options at the HIGH END of his product funnel. For $10k or thereabouts, you'll be able to spend 3 full days looking over his shoulder at his home office. This will be for just one person. THREE DAYS, one person only, $10,000.
Another option will be a two-day event limited to just 3 people total. This event will be priced somewhere around $4-5k. Each person will pay less and they will have two as opposed to THREE days with him. His total gross revenue will be around the same or a bit higher than the 3 day event.
Lastly, he'll have a one day event restricted to just 2 people. He's thinking about a price of around $2,000. Total gross? $10k.
These are a number of the high priced options that Bill will be offering. He'll also be offering numerous lower priced products as well. Audio, video and text.
His site: Real Voice Over Training.
Andrew Hymers is an engineer who a number of years back went through a painful divorce. He has written hundreds of thousands of words (in article form) that will help other younger, entrepreneurial men get through exactly what he went through.
Andrew will use a slightly different model. HIS goal is to get people to find him and then higher him on an hourly basis to help THEM get through THEIR difficult situation.
He'll be putting up all his writing into a Word-Press site. He WON'T put it up all at once. If he did, the search engines would most likely penalize him. Too much content put up at once makes the search engines suspicious!
Over time, more and more posts will go up in 4 main categories that he's devised. In due time, the search engines will find his site. From this "authority" site he'll be able to send people to his "sales site". The sales site will be all about getting people to pay him in advance for some one-on-one counseling via phone or skype.
Again, another model. Can you use this one? Maybe that's the right one for you.
Another participant at the bootcamp was Michael Potter. He is an asset protection attorney. He is trying to get more people to attend his seminars. At the seminars he gives them great information related to the topic and HIS GOAL is to get people use his services as a lawyer to set up ENTITIES which they need to protect themselves.
This is yet another model in the information marketing field.
What's your business? What should YOUR model be? It all depends. Ask some questions if you need clarification!


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About me
My name is Christine and I rescue and rehabilitate all garden birds, including the Corvids, that have been found injured or abandoned. These birds are then released back to the wild. I provide a safe haven for Hens that have been injured or abused. I will take Budgies that have been abused or that can no longer be cared for. I do not have the facilities to take in Seabirds, Swans, Raptors or Owls, Geese or Ducks.
Wild Birds
Wild birds are naturally frightened of humans and when injured or stressed they need a secluded environment with as little disturbance as possible to give them the best chance to survive. Biosecurity measures are in place, therefore the Sanctuary is not open to the public.
The Sanctuary is a registered charity run by myself with the help of the trustees, namely, Pauline Mclynn, Josephine Gurney and Sheila Archbold. We thankfully received a grant of €3,000 from the Department of agriculture but rely on fund raising and anyone that would like to donate or provide grain, seed, wild bird seed, fat balls etc.. Please contact me if you would like to donate. There is a donate button on the contact page. Any help, however small, is very much appreciated.


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What's the story for topic moderation?
It seems that right now topics are curated by site owners. No idea about moderation - are there any mechanisms to prevent topic abuse? From the very obvious spam, which should be easy to detect (spammers going broad and wide) to the nuanced "this thing doesn't belong here". Historically, the best job at doing this has been done by the topical communities themselves. What are the Cake plans here?


text: Dr Anu Mathew completed the Victorian ophthalmology training program and was awarded Fellowship of the Royal Australian and New Zealand College of Ophthalmologists in 2015. She has undertaken further advanced training in strabismus and paediatric ophthalmology at the Royal Children's Hospital, the Royal Victorian Eye and Ear Hospital and Moorfields Eye Hospital in London. Dr Mathew currently has public appointments at the Royal Children's Hospital, the Royal Melbourne Hospital and the Royal Women's Hospital. Her surgical expertise covers most areas of strabismus and paediatric ophthalmology.


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Oct 25, 2017
Tpo Waterproof Membrane for Flat Roof with Excellent Weather Resistance Basic Info
Model NO.: JLY-TPO
Ceiling Tile Shape: Roll
Waterproofing Membrane Thickness: 2.0mm
Surface Treatment: One Side with Fabric
Certification: ISO, CE
Usage: Outdoor
Transport Package: Plastic Bag
Waterproofing Membrane Origin: China
Type: Waterproof Roll Material
Material: Tpo
Ceiling Tile Type: Tpo
Function: Waterproof
Waterproofing Membrane Color: White
Waterproofing Membrane Specification: 1.2x2000x20000mm
HS Code: 3921909090
Tpo Waterproof Membrane for Flat Roof with Excellent Weather Resistance Product Description
TPO waterproof membrane for flat roof with excellent weather resistance
1.Waterproofing Membrane Properties:
1)Excellent resistance to low and high temperature, keep flexibility at minus 40oC and keep mechanical strength at high temperature.
2) Good resistance to chemical attack; resistance to the corrosion of acid, alkali, salt,animal and vegetable oil , and resistance to alga, microbial growth.
3)Excellent resistance to root, and also can be used for planted roof.
4)TPO waterproof membrane is weldable, excellent weather resistance and aging resistance.
5)Resistance to heat aging, good dimensional stability.
6)TPO does not contain plasticiser, chlorinated polymer and not release toxic gases during welding the TPO waterproof membrane.
2.Tpo Waterproof Membrane for Flat Roof with Excellent Weather Resistance Specification:
|Product name
|TPO waterproof membrane
|1.2mm, 1.5mm, 1.8mm, 2.0mm
|1.0m, 1.5m, 2.0m
|20m, 30m
|Remarks: Other sizes are customized according to customer's requirement.
3.Tpo Waterproof Membrane for Flat Roof with Excellent Weather Resistance Technical index
4. Type of TPO waterproof membrane:
H type: Homogeneous TPO waterproof membrane without fabric backing or reinforcement.
L type: backing fabric
P type: reinforced with polyester fiber mesh
5.Packing: packing with plastic or woven bag.
6. Application & Construction:
Suitable for roof waterproof projects of industrial and civil building, public building:
H type: used as flashing materials;
L type: used for roof waterproof system by full adhibiting method, and border adhibiting method;
P type: used for roof waterproof system by mechanical fixing method and bordeer adhibitinh method.
7. The workshop of our factory
1.Factory or trading company?
Factory. We are professional manufacturer.
2.Sample and quality
The free samples can be provided for quality inspection. And the customer pay the sample freight. The delivery cost will be returned after placing the order.
3.Carrier and delivery time of sample
Carrier: DHL, UPS, FedEx, TNT or as customer's requirement.
Delivery time: 1-3 working days
9. 24 Hours Service
Any questions, please feel free to contact us. We will offer you professional pre-sale and after sale service.The professional advice also can be provided by our engineer, if you need.
Taitou Industrial Zone, Shouguang City, Shandong Province, China


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Cameron Diaz Videos by Popular
Celebrities Who No Longer Have That 'It' Factor
These stars were everything in their day but the sun is slowly setting on those times. tags: adeleashton_kutchercameron_diazhollyscoopjessica_bielkate_hudsonlady_gaga
Cameron Diaz sagt, Botox hat ihr Gesicht seltsam verändert
Cameron Diaz ist eine der heiesten Hauptdarstellerinnen in Hollywood. Aber sie gab zu, dass sie das ein oder andere unternommen hat, um ihr ... tags: BotoxCameron_Diazdas_Body_BookGesundheitJungendRatschlagSchönheit
Cameron Diaz admits using botox!
Cameron Diaz confesses she's tried to smooth out her wrinkles tags: botoxCameron_Diazfilmfitnesshealthskinthe_body_book
Cameron Diaz & Lena Dunham Snap A Smiling Selfie While Appearing On Good Morning America
Lena Dunham and Cameron Diaz were both on Good Morning America yesterday, and they took an adorable pic together to commemorate their ... tags: Actors_from_CaliforniaAmerican_film_actorsCameron_DiazCharlies_Angels:_Full_ThrottleGood_morning_americaLena_DunhamPhoto_sharing
Cameron Diaz Says No to Botox
Botox is a popular beauty treatment in Hollywood and Cameron Diaz is sharing her thoughts on this cosmetic procedure. tags: acnebotoxcameron_diazmarianna_hewittmodamobskincaresmile_lines
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Pubis poilu : le conseil de Cameron Diaz
Cameron Diaz na jamais hsit mettre en avant ses opinions et lactrice ge de 41 ans en a mme un sur les poils pubiens tags: épilationbody_bookcameron_diazpoiluspubisvaginzoomin
ET FIRST: Cameron Diaz Regrets Botox
Actress Cameron Diaz has admitted having injections of Botox in the past, but exclusively tells ET that she's not a fan of the anti-aging ... tags: Botoxcameron_diazET_Online
In Her New Book Cameron Diaz Encourages Women To Keep Their Pubic Hair
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Actress Cameron Diaz released her health guide The Body Book last month, and in the section In Praise of Pubes the 41-year-old explains why ... tags: ActressCameron_DiazCameron_Diaz_A_Fan_Of_Pubic_HairEntertainmentEntertainment_NewsIn_Praise_Of_PubesThe_Body_Book
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text:
Authored by Shannon Adamcik
To buy a Print Book, go to https://www.createspace.com/3917769
This is a true story and no one can tell it better than the people who lived it. A crime reporter can look at the details of a case, but they cannot tell you how it feels to live through it. I can and I did. I used the pre-trial and trial transcripts, copies of the police reports, the autopsy and DNA reports, and DVD recordings of all of the evidence in the case. I've done copious research. But more importantly, I take readers step-by-step through what it feels like when your 16-year-old son is accused of first-degree murder; all the odds are stacked against him; and his defense is in the hands of attorneys you can't fully trust to come through for you.
Sixteen-year-old Cassie Jo Stoddard agreed to house sit for relatives on the weekend of September 22, 2006. It was something the teenager had done before…but this time something went terribly wrong. When the family returned home at the end of the weekend they found Cassie lying on their living room floor brutally stabbed to death.
Detectives focused on two of Cassie's classmates who had briefly visited her on the night that she was murdered: Torey Adamcik and Brian Draper. Initially both boys denied any knowledge of the crime, but after two separate interrogations, Brian Draper told detectives a chilling story of murder straight out of a horror movie. The two boys were immediately arrested, and a shocking videotape was discovered that seemed to depict the two teens not only planning the cold-blooded murder, but celebrating it.
Community outrage was strong and immediate. The public demanded justice. But was the video actually what it appeared to be: a cold-blooded documentary that detailed the plotting of Cassie's murder; or something else entirely? Could anyone uncover the truth in time and convince a jury that sometimes things aren't always what they appear to be?
The Guilty Innocent is narrated by Shannon Adamcik, mother of Torey, one of the accused boys. It takes readers behind the scenes of a trial where prosecutors cared more about public opinion than truth, defense attorneys, who had never argued a murder case, were in over their heads, and a young boy's life hung in the balance.


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Monthly chaos invasion
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Beefy's Rant - One WoD "rule" which in my POV, simply makes ZERO sense
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Beefy's Rant - One WoD "rule" which in my POV, simply makes ZERO sense
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quest bug in 386 cave
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text:
Map of Great Spas of Europe (Czechia)Load map
The coordinates shown for all tentative sites were produced as a community effort. They are not official and may change on inscription.
Community Reviews
The Czech part of this nomination is sometimes called as the West Bohemian Spa Triangel. It consists of Karlovy Vary, Marianske Lazne and Frantiskovy Lazne. This part of Czechia was inhabited by German minority before WWII, thus, the spas are also known by their German names: Karlsbad, Marienbad and Franzensbad. Nowadays, Karlovy Vary is however very popular for Russian minority.
The Triangel as well as spa Luhacovice in the eastern part of Czechia (Moravia) have been included to T-list as separate sites for quite a long time. Luhacovice was deferred in 2008 and eventually "sacrificed" on the altar of trans-European nomination called Great Spas of Europe. The West Bohemian Spas emanate an international ethos for centuries. They certainly have the OUV, and they should have been already incribed. They are generaly appreciated as the symbol of spa culture by everyone in Czechia as well as abroad. Thus, I do not understand why the Czech nomination needs the help of western friends to be incribed... It reminds me one comment from the forum that by application of this approach the entire Europe minus Dresden will be inscribed soon as the trans-natinal cultural landscape of a global importance...
Luhacovice spa is also special but it is built in national style and it has mostly national connotations such as the influence of composer Leos Janacek.
The West Bohemia Spa Triangel is famous for natural springs (tasting higly recommended + liqueur Becherovka + delicious spa wafers), colonnades in Art Nouveau and modern styles, and relaxed atmosphere. Karlovy Vary is also known for the Film festival with tradition over 50 years.
(1) Karlovy Vary spa is the largest and the most famous from the Czech component, it is located in a deep, green valey of river Tepla (it means warm river in Czech). It was founded in midle ages and among several historical structures there, barroque church of St Mary Magdalene should be mentioned. However, it mostly consists of hotels and buildings for balneology purposes from 19th and 20th century including brutalist landmark - hotel Thermal (built 1967-1976). The most spectacular hot spring is Vřídlo geyser inside modern colonnade from 1975. It was under reconstruction during my last visit in 2019, and geyser "does not work" now. However, three hot springs inside the building are still acccessible - PHOTO. (2) Marianske Lazne spa is not such compact and intact structure as Karlovy Vary, but you can find very fine cast-iron colonade there. (3) Frantiskovy Lazne is the smallest but the most intact component recognized by yellow facade of 18th-19th century buildings.
The Spas are nominated for 2020, and I expect smooth inscription!
If you are in the area, there are quite a lot other places of interest: (i) Slavkovsky Les protected landscape area covers forested montaneous plateau with unique serpentinite rocks, numerous natural mineral springs, beautiful lakes such as Kladska, and areas with unique flora around vilage Prameny (2) Lazne Kynzvart small spa town with several springs and the chateau with fine collections, (3) the castle in Becov nad Teplou with wonderful Reliquary of St. Maurus from 13th century, (4) Soos natural reserve - incredible swamp area, etc...
Community Likes
Site Info
Site History
2014 Added to Tentative List
Site Links
The site has 3 locations


text:
Arsene Wenger turned down the chance of making a sensational Premier League return with Fulham before the Cottagers appointed Claudio Ranieri.
Fulham confirmed on Wednesday morning that Ranieri has succeeded Slavisa Jokanovic, who has paid the price for the club's terrible Premier League start that sees them bottom of the table. In their statement confirming Ranieri's appointment, Fulham confirmed they had considered and spoken to a number of candidates and Telegraph Sport can reveal one was Wenger.
READ ALSO: EPL! Manchester United Foward Alexis Sanchez Considers A Move To Real Madrid [READ]
But Wenger politely declined the opportunity to speak to Fulham, sticking to his vow not to manager another Premier League club after leaving Arsenal.
It is understood Wenger is waiting for the right opportunity abroad, with Bayern Munich likely to be a job that would interest the Frenchman if it became available. Bayern are currently fifth in the Bundesliga table, seven points behind Borussia Dortmund, who they lost to last weekend.
Fulham owner Shahid Khan gets on well with Arsenal owner Stan Kroenke, who would have given Wenger a glowing reference despite his departure from the Emirates.
Kroenke has always been a big fan of Wenger, despite finally agreeing the time was right for the 69-year-old to leave Arsenal last summer.
READ ALSO: INTERESTING! Chelsea Striker Giroud Set To Feature In Upcoming Hollywood Movie 'Spider Man' – [SEE]
In their statement on Ranieri, Fulham wrote: "Khan said he and his son Tony Khan, Vice Chairman and Director of Football Operations at FFC, considered and spoke with a number of potential candidates within the past week, with Ranieri becoming their mutual and obvious choice. Tony Khan and his staff will continue to oversee First Team player recruitment and contractual agreements, and he will work closely with Ranieri."
SEE ALSO: KNOCKED OUT: Why Atletico's Costa Won't be able to Face Barca [OPEN]
If you wish, you can also send your stories, pictures and videos to our
Whatsapp number: 09098070080
or any of our Social Media platforms:
Facebook: Facebook.com/Thesheetng
Twitter (tweet at us or DM): @thesheetng
Instagram: @thesheetng


text:
8-12 Alder Hills
BH12 4AL Poole
United Kingdom
BH12 4AL Poole
United Kingdom
Phone: 01202413611
Website: www.pulse-eight.com
Social Media Links:
ProfileBased in the UK, Pulse-Eight are leading developers of Video/Audio control and distribution systems, we´ve been around since 2010 and have a wide collection of award winning hardware to our name. Not all of our hardware is sold directly by us, we manufacture for others and we can do the same for you.Featuring our neo HDBaseT matrix range, HDMI extenders, HDMI splitters and much more.
- Commercial
- Home Automation
- Home Cinema
- Media Distribution
- Residential


text:
Beard Moisturiser (Bleu Noir)
BLACK BEARD | BEARD MOISTURISER(Bleu Noir)
Beard Moisturiser is specially formulated to effectively cool, refresh, soothe, hydrate and de-stress your beard & skin. Our soothing balm helps to provide excellent antiseptic and stimulating qualities, plus having a wonderful rejuvenating and regenerative effect on the beard & skin.
Hold: Soft
Scent: Bleu Noir
Application: Gently massage the balm into the skin on and around the shaved area until fully absorbed. Our Beard Moisturiser is specially formulated to effectively cool, refresh, soothe, hydrate and de-stress your beard & skin.
Recommended aftercare shampoo to get out all wax and impurities: Anchor Jack Beard Shampoo.
Return & Refund Policy:
Thank you for shopping at our Site.
If you are not entirely satisfied with your purchase, we're here to help.
Please contact me if you have any problems with your order.
Please contact me within 7 days of delivery.
You have 14 calendar days to return an item from the date you received it.
We don't accept exchanges or cancellations, sorry.
To be eligible for a return, your item must be unused and in the same condition that you received it. Your item must be in the original packaging. Your item needs to have the receipt or proof of purchase.
REFUNDS: Once we receive your item, we will inspect it and notify you that we have received your returned item.
We will immediately notify you on the status of your refund after inspecting the item.
If your return is approved, we will initiate a refund to your credit card (or original method of payment). You will receive the credit within a certain amount of days, depending on your card issuer's policies.
SHIPPING: You will be responsible for paying for your own shipping costs for returning your item. Shipping costs are nonrefundable. If you receive a refund, the cost of return shipping will be deducted from your refund.
CONTACT US: If you have any questions on how to return your item to us, contact us.
Shipping Info:
Standard Delivery
- Anchor Jack offers FREE standard delivery on all orders over 1000,-
- Once we have accepted your order, please allow 3 - 5 business days for us to process your order prior to us sending your order out for delivery.
- Customers will be contacted directly should we be unable to process orders in this time-frame. During peak or seasonal periods, please allow up to 6 business days after we have accepted your order for your order to be processed and then sent out for delivery.
- Please get in touch if you are unsure about our shipping prices.
Thank you.
Ingredients: Aqua, Hemp Oil, Simmondsia Chinesis, Polythylene, Sodium Polyacrylate, Phenoxyethanol, Ethylhexylglycerin, Parfum.


text: The VisuNet GMP product portfolio by Pepperl + Fuchs includes simple monitors, remote monitor systems with Ethernet connection to the host PC, as well as complete PC systems. They offer a variety of mechanical mounting options and are available in different configurations, such as single and twin-monitor systems. All models are equipped with a 19" display, optionally available as a touch screen. They feature stainless steel cabinets according to protection rating IP65. Remote monitors and PCs offer RS232, PS/2, Ethernet, as well as USB interfaces. The design of the VisuNet GMP was tailored especially for the use in life science industries. Selected materials, surfaces, as well as the overall architecture of the systems were designed to prevent the accumulation of dirt and fluids. In addition, the support safe and easy cleaning, maintenance, inspection and repairs of all mechanical components. The same is true of disinfection, pasteurisation and sterilisation.


text:
860 Lee Drive, Ponchatoula LA 70454
Call 888-821-8250 to connect with the builder right now!
Save this Home
to the NHS Mobile App
Tour This Home
860 Lee Drive, Ponchatoula, LA 70454
Sales Agent Kelly Turner
Hours Monday - Saturday 10AM - 6PM Sunday 12PM - 6PM
Same-day scheduling unavailable online. To request a same-day tour, please call the builder #phoneNumber
Please be aware that current conditions may impact hours of operation.
Valued Partners
Want to see more details?
Book a virtual tour with a builder expert.
June Incentives
Use DSLD Mortgage and DSLD Title to close on your DSLD home and receive up to $5,000 towards your closing costs. (only up to $4,000 for North Louisiana communities). See your Builder Sales Representative and Mortgage Loan Originator for more details!Learn more »
- Open Floor Plan - Four Bedrooms, Three Bathrooms - Brick and Stucco Exterior - Separate Breakfast and Dining Room - Wood Floors in Living and Dining Room -Fireplace - Boot Bench and Drop Zone in Mud Room -Crown Moulding - Recessed Lighting in Kitchen and Living -Double Master Vanity -Separate Master Shower -Walk-In Master Closet -Two Car Garage -Covered Rear PatioVisit the Builder's Website
860 Lee Drive
Ponchatoula, LA 70454
Visit and take the app with you!
Click to get the NHS Mobile App
Welcome to The Crossings! This community is conveniently located off Hwy 22 in Downtown Ponchatoula off of Yokum Road. This location is secluded and tucked away while being surrounded by beautiful trees, but is still conveniently located to local shopping and the interstate. The entrance to this neighborhood welcomes you with beautiful Live Oak trees and landscaping. You are only minutes away from Downtown Ponchatoula which offers many things to do including some of the best breakfast spots, local restaurants, shopping, and most importantly the Strawberry Festival. The Strawberry Festival is a great event for everyone! It is held in early April at Memorial Park in Ponchatoula, It offers tasty food, fun rides, games, music, and of course strawberries. This is one festival you don't want to miss! You can choose between a large variety of 3 and 4 bedroom floor plans in The Crossings. Our floor plans offer a variety of amenities such as 3 CM granite countertops, under mount sinks, wood flooring in the living area and halls, granite around the fireplace, upgraded lighting, framed mirrors, and so much more. You can also choose from a large selection of colors and upgraded options to give your new home your own personal touch. Call today so we can take a tour of the neighborhood!
Area Schools
Actual schools may vary. Contact the builder for more information.
Ready to build your new home in the most vibrant state? Pick your favorite city, DSLD Homes builds new construction homes all over the pelican state! Here in the Deep South, we have a rich history with a unique blend of cultures. Achieve your Associates, Bachelors or Masters at one of the thirty-six, four-year Colleges and Universities. Enjoy a festival every weekend, or take one of the thousands of great walking tours to catch a glimpse of the rich history, heritage, and culture Louisiana brings to the table. Not only does this state have numerous educational opportunities, but it also has unlimited jobs. Looking to work in the hospitality industry, entertainment or join the corporate world there are a variety of options here in Louisiana. Spend your time off catching beads and yelling "throw me something mister," at the annual Mardi Gras parades, stroll through one of Louisiana's serene state parks, attend the Jazz Fest or one of the many festivals throughout the state. Find your place in this lively state while exploring the amazing food, fun casinos, exciting nightlife, historic museums, plantations, art galleries, and recreation. You will love the excitement, opportunities, and history that Louisiana has to offer you! Most importantly, you will love your new DSLD Home! Offering a variety of floorplans and amenities we're sure to have something that fits your family. Model homes are open daily for you to see the quality and value of our homes, talk with a Builder Sales Representative, and explore the community itself. Come visit one of our beautiful Louisiana communities and find your new home in this vibrant state.
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text:
Life's been good.
Here's what I've been up to lately:
It's been a pretty mellow but sweet summer, and we are enjoying these warm California days. Happy weekend to ya :).
Cajun Chicken Alfredo Penne
Serves 4
*Cajun Seasoning Recipe
adapted from this recipe on chowhound.com
Makes about 3-4 tablespoons of seasoning, though the recipe only calls for 1 tablespoon.
Nice post! I like this recipe, it's very healthy and easy to do. Thanks for sharing!
Thanks for posting this recipe Jo! I was craving creamy pasta recently and remembered the yummy pasta you made for us for MealBaby. I looked at your website to see if you posted it and made it. It is delicious :o).