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Gene cloning shows the alpha-toxin of Clostridium perfringens to contain both sphingomyelinase and lecithinase activities
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The plc gene encoding the alpha-toxin (phospholipase C), an important virulence factor of Clostridium perfringens, has been cloned, sequenced and expressed in Escherichia coli. Transcriptional analysis of mRNAs produced in vivo by C. perfringens and E. coli, and in vitro using purified RNA polymerase from C. perfringens revealed that plc is transcribed constitutively from a single promoter situated about 100 nucleotides from the coding sequence. A T7 expression system was used to overproduce alpha-toxin in E. coli; enzymological studies with the amplified plc gene product unambiguously demonstrated that both lecithinase (phospholipase C) and sphingomyelinase activities were associated with this 43 000 dalton cytotoxin. The 370-residue alpha-toxin is haemolytic and shares sequence and functional homology with the two components of Bacillus cereus haemolysin, cereolysin AB, in which phospholipase C and sphingomyelinase activities are associated with different polypeptides.
Key wordsExotoxins Phospholipases Gene regulation Gangrene
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Celebrating the life of Mattie Adams Justice
Be the first to share your favorite memory, photo or story of Mattie . This memorial page is dedicated for family, friends and future generations to celebrate the life of their loved one.
We ask on behalf of the family that you keep your comments uplifting and appropriate to help all who come here to find comfort and healing.
Mattie Adams Justice, 85, of Lexington was born March 07,1933 at Branham Hollow in Powell County, Ky. She was the daughter of the late Sadie Branham and Decoursey Adams. Along with her parents, Mattie was preceded in death by her loving husband, Frank D. Justice, Sr.; two brothers: John Calvin Adams and Decoursey Adams; three sisters: Opal Venable, Marie Lewis and Ruby Griggs.
Survivors include: Two sons; Dr. Frank D. Justice II (Allyson) of Pikeville, Jason Ledford Justice (Kim) of Lexington. Two daughters; Bridget Justice of Lexington, Ky and Karla Justice Corbin (Paul) of Pikeville, KY. One sister; Stanley Horton of Powell County, Ky. Nine grandchildren; Brittany Blair Boggs (Shawn), Adam Blair, Jessica Justice, Don Paul Corbin (Kari Lynn), Matthew Corbin (Heather), Franklin Drew Justice, Kaitlyn Justice Newsome (Jordan Paul), Morgan Taylor Justice, Lincoln Ashford Justice; Eleven great grandchildren; Jackson Boggs, Mattie Boggs, Addison Boggs, Brady Corbin, Tristen Corbin, Scott Douglas Corbin, Kai Corbin, Justice Beall, Sawyer Beall, Jacobson Beall and Remington Corbin.
A faithful servant of Christ; Mattie along with her husband of 59 years, Frank, founded and operated the following businesses: Justice Shoe Store, Mari Dru Shop, Jerry's Restaurant, Velocity Market, Long John Silvers, Mac Mining, Mac Coal Sales, Frank Justice Furniture, Triple J Farm and Dell Ridge Farm. She will be remembered for her generous gifts to students, local education institutions, church missions work and Habitat for Humanity through the Frank and Mattie Justice Charitable Foundation.
Mattie was honored in the 2013 Distinguished Educators Hall of Fame for the University of Pikeville. She was recognized in 2002 with the Joseph W. Kelly Award from the Kentucky Board of Education. She was an honorary distinguished alumnus for Pikeville Independent Schools in 2002. She received a Doctor Humane Letters Degree from Pikeville College in 2004 and an Honorary Alumni Award from the University of Pikeville Alumni Association in 2011.
Expressions of sympathy may be made to: Mission Works % Main Street Church of Christ, PO Box 2747, Pikeville, KY 41502 or the University of Pikeville, 147 Sycamore Street, Pikeville KY 41501
To plant a tree in memory of Mattie Adams Justice, please visit our Tribute Store.
Previous Events
2280 Hume Road
Lexington, KY 40516
Visitation for Mrs Justice will be Monday March 4, 2019 at North Lexington Church of Christ, located at: 2280 Hume Road in Lexington, Kentucky, beginning at 5:00 P.M. and going until time for services at 7:00 P.M.. She will be brought back to Pikeville, Kentucky, Main Street Church of Christ Tuesday for a visitation beginning at 11:00 A.M. until time for funeral services at 2:00 P.M. burial will follow at Johnson Memorial Park
198 Main Street
Pikeville, KY 41501
The visitation will begin at 11:00 A.M. at Pikeville Main Street Church Of Christ on Tuesday March 5, 2019, and go until funeral service time at 2:00 P.M., Burial will follow at Johnson Memorial Park in Pikeville, Kentucky.
Cemetery Details
South Mayo Trail
Pikeville, KY 41501

subset: commoncrawl


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An absolutely STUNNING piece of rolling hills in Henderson County. This 295 (- +) acre ranch has a gorgeous lake! This property has about 50% woods and 50% open, it is the perfect combination of a hunting, fisherman or farmer's dream. The cabin is nestled in the woods that overlooks the lake. There is already electricity on the property ran to the cabin which would make building a dream house a breeze. There is a well on the property as well as water available at the road. There are two entrances from the county road or one entrance from the FM road. Lots of memories and opportunity to be made with this piece of property.
Users may not reproduce or redistribute the data found on this site. The data is for viewing purposes only. Data is deemed reliable, but is not guaranteed accurate by the MLS or NTREIS.
Listing information last updated on October 15th, 2019 at 6:45am CDT.


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Gabrielle Rose
In this week's episode Naomi Snieckus sits down with Gabrielle Rose at the 2018 Toronto International Film Festival to discuss her extensive acting career, aging in the entertainment industry, choosing projects for her artistic interest, and learning lessons from every struggle and downfall she's ever encountered. For more on Gabrielle follow her on Twitter @GabrielleRose79.
If you loved this episode feel free to leave a comment on our iTunes or even better share it! For more Firecracker Department podcasts be sure to subscribe on iTunes and for more awesome Firecracker Department content follow on Twitter and Instagram @firecrackerdept.
Gabrielle Rose's acting credits include Unspeakable (2019), The Good Doctor (2018), Kingsway (2018), Sweet Virginia (2017), The Man in the High Castle (2016), Once Upon A Time (2011-2016), Proof (2015) and countless other roles.


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According to the link, below 10.5 Client version of DB2 can only work with Server version 10.5 or later. http://www.ibm.com/support/knowledgecenter/SSEPGG_10.5.0/com.ibm.db2.luw.qb.client.doc/doc/r0009731.html
In other words, thlis client should not work with Server 9.7. Nevertheless I managed to create a bind file with a 10.5 Client and make it execute (connect, run etc) OK with a 9.7 DB server. How can this be possible? Is this not supported officially?
Answer by Colm Forkin (28) | Nov 18, 2016 at 05:19 AM
Just foudn answer after posting..
The following clients and drivers can access a DB2 Version 10.1, Version 10.5, DB2 Version 9.7 or DB2 Version 9.5 server:
IBM® Data Server Client Version 10.5 IBM Data Server Runtime Client Version 10.5 IBM Data Server Driver Package Version 10.5 IBM Data Server Driver for ODBC and CLI Version 10.5
96 people are following this question.
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SQL Error -20071 seen when running createProcedure_Advanced.sql on DB2 zOS 1 Answer
Calling db2ext.textSearch procedure fails due to CTE0243 when using persistent cache table 1 Answer


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Hi There! Is this Your First Time?
Did you know if you Register you have access to free search tools including the ability to save listings and property searches? Did you know that you can bypass the search altogether and have listings sent directly to your email address? Check out our how-to page for more info.
- Price$317,900
- Beds3
- Baths2½
- SQ. Feet2,131
- Acres1.13
- Built2007
500 Fawn Ct, Burns
NEW PRICE!!! Wonderful Home Sitting on A Plush 1.13 Acre Level Lot. Charming Covered Front Porch. Hardwoods in Dining Room and Foyer. Vaulted Ceilings and Gas Fireplace in LR. Natural Light Boasting Throughout. Newer Wood Blinds. Spacious Kitchen with Custom Cabinets and Beautiful SS Appliances (2 yrs old). Kitchen Has Heated Tile Floors. Freshly Painted Master Suite with Large Walk-In Closet. Roof 2 yrs old. Abundantly Sized 2 car garage. Expanded Concrete Drive.
Essential Information
- Price$317,900
- Bedrooms3
- Bathrooms2.50
- Full Baths2
- Half Baths1
- Square Footage2,131
- Acres1.13
- Year Built2007
- TypeResidential
- Sub-TypeSingle Family Residence
- StyleCape Cod
- StatusUnder Contract - Showing
- Parking Spaces2
- # of Garages2
- GaragesAttached - Side
- RoofAsphalt
- ConstructionBrick,Vinyl Siding
Additional Information
- Date ListedJuly 10th, 2019
- Days on Market33
Community Information
- Address500 Fawn Ct
- Area15 - Dickson County
- SubdivisionBuck Hollow Sub Phase Ii
- CityBurns
- CountyDickson County
- StateTN
- Zip Code37029
- Interior FeaturesAir Filter,Ceiling Fan,Extra Closets,Storage
- AppliancesGarbage Disposal,Microwave,Dishwasher
- HeatingCentral
- CoolingCentral
- FireplaceYes
- # of Fireplaces1
- # of Stories2
School Information
- ElementaryStuart Burns Elementary
- MiddleDickson Middle School
- HighDickson County High School
Listing Details
- Listing OfficeUnited Real Estate Music City
The data relating to real estate for sale on this web site comes in part from the Internet Data Exchange Program of RealTracs Solutions. Real estate listings held by brokerage firms other than Synergy Realty Network are marked with the Internet Data Exchange Program logo or thumbnail logo and detailed information about them includes the name of the listing brokers.
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Copyright 2019 RealTracs Solutions.
Listing information last updated on October 19th, 2019 at 2:56pm CDT.


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Written on train 3rd September 2013
You were our voice, our Ulster voice.
You followed from Mossbawn to Sandymount
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Criss crossing images, thoughts and words
Into woody briar sticks of language.
Now you yourself consort underground
With the great Horseman himself.
Noli temere: Hail & Farewell.


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Capturing the state of the Soviet-built Equestrian center in Charbakh, Yerevan. The center is active and functioning, but in dire need of additional funding.
Many of the horses have fallen ill through the winter months, but due to a lack of veterinarians in the region who specialize in horses paired with insufficient funds, fatalities among the horses are rapidly rising and the state of the facilities is simultaneously declining.


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Resurfacing of a 3.4-mile portion of Creek Road (County Route 636) in Delran, Mount Laurel and Moorestown townships is expected to begin on Aug. 5.
The project involves pavement milling and overlay of Creek Road from Burlington County Route 613 in Delran to Centerton Road in Mount Laurel. The work is expected to take about three weeks to complete.
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The work is being done by American Asphalt Co., Inc., West Collingswood, New Jersey, as part of Burlington County's 2017 State Funded Overlay Program (2018-2019 Construction).
A total 24.8 miles of roadway in 15 municipalities are being resurfaced through shared State & County funding.


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"The little things? The little moments? They aren't little. ~ Jon Kabat-Zinn
Observed :: Ten Things
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grass greening,
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gray skies, thickening, blocking out the sun.
a chill in the air, winter hesitant to leave.
lady cardinal, perched on a fence,
eyes us warily as we stroll by.
next to seed pods opened a season ago.
we head inside before the sleet starts to fall.


text:
Jerwood Foundation made a grant of £900,000 towards the restoration and modernisation of The Natural History Museum's magnificent Gallery 26. Originally The Shell Gallery, it had been bomb damaged in the Second World War and covered with cladding when it reopened in the 1950s. Following restoration by Robert Warren Architects the renamed Jerwood Gallery was opened in September 1999 by Lord Palumbo.
The Jerwood Gallery, with its Waterhouse terracotta work, triple arched entrance and stained glass windows at front and rear, provides an impressive exhibition venue for the Museum's arts/science exhibition programme. This incorporates both contemporary visual art and the Museum's own historical natural history art collection.
In October 2018 the Jerwood Gallery was transformed into a temporary theatre, hosting the European premiere of The Wider Earth, a magical and ingenious theatre production whic brought to life the intrepid journey undertaken by the 22 year-old Charles Darwin on HMS Beagle. The Jerwood Gallery is currently displaying artist Luke Jerram's touring installation Museum of the Moon until 1 January 2020.
Originating from collections within the British Museum, The Natural History Museum opened in South Kensington in 1881 on the site of the 1862 International Exhibition. Alfred Waterhouse's landmark building in the German Romanesque style is home to around 70 million life and earth specimens in five main collections: Botany, Entomology, Mineralogy, Palaeontology and Zoology.


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IZET company is one of the largest Internet service providers in Russian Fedeation. Since 2010, the company provides high-speed data transmission for Velikiy Novgorod, Chelyabinsk, Cherepovets and Yaroslavl.
Read more
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Good to know
|Distance to city centre
|6.0 km
|Location rating
Deluxe rooms
Executive rooms
Double rooms
Standard rooms
Queen rooms
Leisure rooms
King rooms
|"Location was great, pillows were perfect."
|(in 289 reviews)
|"Very comfortable and clean room!"
|(in 224 reviews)
|"Friendly staff and great location."
|(in 182 reviews)
|"Elevators were slow and crowded"
|(in 39 reviews)
|"No fridge in room!"
|(in 20 reviews)
Traveller rating
Time of year
Traveller type
Show reviews that mention
|battery park
|in 50 reviews
|elevators were slow
|in 211 reviews
|financial district
|in 50 reviews
|fridge in the room
|in 51 reviews
|great location
|in 694 reviews
|hudson river
|in 23 reviews
|long time
|in 29 reviews
|near subway
|in 201 reviews
|room with a view
|in 233 reviews
|small room
|in 696 reviews
|staff was friendly
|in 428 reviews
|statue of liberty
|in 32 reviews
|waiting time
|in 38 reviews
|walking distance
|in 34 reviews
|world trade
|in 80 reviews
ExcellentOct 201910.0
Had the buffet breakfast all of the 4 mornings. It was adequate and nutritious. A bit crowded several mornings, but, otherwise, no problems or complaints. The car company that the concierge suggested was perfect for the few times we needed transportation. Otherwise, we were able to walk everywhere we wanted to go.
There was nothing that was problematic or deserving of a complaint
OkayOct 20195.0
Location wise GOOD
Breakfast very poor
GoodOct 20197.9
People are good here great staff except one of the gentleman (Indian fellow) who works at front desk night shift, he doesn't belong there! He should be put in back office! No customer relationships
A staff who works at night shift front desk! He is rude
OkayOct 20195.4
The location was convenient to public transit.
Tight quarters, no fridge, no iron, no coffee in room, room service never came when ordered, ac and elevators worked sporadically.
GoodOct 20197.9
The hotel was nice overall and has 50 floors, which is a big plus if you visit new york.
The rooms are tiny, there wasn't a closet in the room, which i found weird. Just a small one for like 5 shirts and our room was apparently for 4 people. The elevators were always full so you had to wait sometimes 10 min to go up or down.
The Holiday Inn Manhattan Financial District provides visitors with one of the most exciting places to stay in New York. Known as the world's tallest Holiday Inn, this hotel is ideal for business or leisure travelers who want to be in the thick of the action.
This hotel is located close to Lower Manhattan's 9/11 Memorial Museum, the Stock Exchange and Wall Street, Battery Park and the Statue of Liberty, so everyone can find something to enjoy here.
The Holiday Inn Manhattan features 492 rooms on 50 floors. Each room offers coffeemakers, luxurious bedding, high-speed internet and flat-screen televisions. For larger groups or families, two-room suites are available.
Guests of all ages will enjoy the delicious food at St. George's Restaurant on-site. Parents don't have to worry about picky eaters here, as kids eat free. It features classic American cuisine, and there's a special menu featuring food with an Asian influence. Beverages such as wine, spirits, beer, and tea complement the menu.
The on-site fitness center features two treadmills, one recumbent bike and an elliptical machine, along with free weights. The gym also features its own water cooler and towel service.
Business travelers can use the ultra-modern 24-hour business center for conducting business, with features such as printing, scanning, copying and office supplies. The Holiday Inn Manhattan is therefore a great choice for both business and recreation.
8.1 Excellent
based on 4,897 reviews
Things to know
- Great view
- Close to public transport
Check in
Check out
Policies vary by room type and provider.
Reviews about Holiday Inn Manhattan-Financial District
Most popular landmarks
- Wall Street 324 m
- National 9/11 Memorial & Museum 374 m
- One World Trade Center 0.5 km
- Brooklyn Bridge 1.6 km
- Ellis Island 2.5 km
- Washington Square Park 2.8 km
About the neighbourhood
Financial District is known for: financial epicenter, stock exchange, high-risesMore
New York travel guide
Learn about New York on KAYAK
Getting Around
|13.7 km
|New York LaGuardia
|14.1 km
|New York - Penn Station
|5 km
|Secaucus Junction, New Jeresy
|7.8 km


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About Lydia
Lydia Smith is a freelance journalist, writer, copywriter and editor who writes with a focus on women's rights, health, mental health, feminism, human rights, workplace issues and wellbeing.
She is based in London but has travelled around the world to tell the stories that really matter, from the closure of the last orphanages in Rwanda, to the rise of LGBT+ rights in Cuba.
Her work can be found in: Cosmopolitan, Refinery29, Quartz, the Independent, i News, the Telegraph, Metro, Huffington Post, Patient, NetDoctor, IBTimes UK, Open Democracy and more.
Lydia has spoken on BBC radio and appeared on panel debates. Her documentary on Romanian orphanages, Ceausescu's Forgotten Children, won a 2017 US Telly Award and was nominated for Best Video Journalism at the 2017 Online Media Awards.
Lydia has a background in news reporting and feature writing. She writes for national and online media, charity and corporate clients.
Please get in touch for rates, details on work experience and clients.
- MA in English Literature at King's College London
- BA Hons in English Literature at the University of Birmingham
- Lydia has had media law training.
- Writing news articles, features, opinion pieces and blog posts.
- Copywriting and editing.
- SEO optimisation.
- Sub-editing for print, online and digital editions.
- Social media management.


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Wilma was a kind and compassionate person who loved Jesus and was always ready to try and help anyone who asked. So sorry for your loss.
Wilma was a woman with a beautiful compassionate heart. All who had the priviledge of knowing her would say she was a woman of great faith, love, and power! Praying for each family member that you will feel the presence of her savior, Jesus, walking through your valley of loss and find esquisite comfort in knowing the peace she walked in. She will be dearly missed as no one could ever take her place! We love her and yet we know her days were lived to the fullest! Love Will, and Christine Armstrong
Derek and Stacey,
Please accept my condolences on your Mom's passing. I remember many hours spent at your house on Grove Street. The conversations and laughter are etched in our memories forever. She was a special lady.
We are so sorry for the loss of your Mom. She was a beautiful lady. We are sorry we can't make it to the funeral parlor..but our thoughts and prayers are with you all tonight.
We are sending our deepest condolences, love & prayers. May your dear mom rest in peace. With love, Jim, Maria, Jimmy & Chris Hall.
Greg, Sorry for the loss of your mother. I wish your whole family well. Linda Oaksford Jurica
Dear family,
I shall miss reading Wilma's beautiful articles that were published in the Leader-Herald. They were very encouraging and meaningful to me.
I knew Wilma from the time I was a child, since she was a very close friend of my sister-in-law. She was always such a sweet and kind person.
I know Wilma is now in the arms of Jesus.
My thoughts and prayers are with you at this very sad time.
About 55 years ago, I sat in a seat next to Wilma at the phone co. I gave her a ride home at least once. Now, passing by the house where she lived, I have wondered what ever happened to Wilma. I remember she had her hands full but always was happy and a good hearted person to work with. Where did the time go? My Sympathy for your loss.
Copyright © 2019


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Latest Weather Blog
US could back 1st pot-derived medicine
COLORADO SPRINGS, Colorado (AP) - The U.S. Food and Drug Administration is expected to decide soon whether to give its first approval to a prescription drug made from the marijuana plant.
But parents who have used other products containing chemicals from the plant to treat their children's severe forms of epilepsy are feeling more cautious than celebratory. They want assurance that existing medical marijuana markets in more than two dozen states won't be harmed if the drug called Epidiolex (Eh-peh-DYE'-oh-lex) gets approved.
In particular, some advocates worry that drugmaker GW Pharmaceuticals has lobbied for changes to several states' definition of marijuana, seeking legal cover for pharmacies to sell cannabis-derived products that have the FDA's backing.
A company representative says GW Pharmaceuticals only wants to ensure its product can reach patients.


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Celebrate National Children's Week with energetic performances, funky dance moves, play-based activities.
Come on an amazing adventure as we strive to understand our magnificent neighbour the Moon.
See the impressive 10m high restored telescope skeleton on display for the first time as it celebrates its 150th anniversary.
Experience the Planetarium after hours for an evening of fully immersive cinema in our new event series especially for adults.
Party Beyond returns to Scienceworks on 16 November with another stellar local lineup!
In pairs of one adult and one child, participants will work together to build skills in STEM (science, technology, engineering and maths).
Inspire your little learner to think big in these early years STEM workshops.
Watch the reconstruction of the iconic Great Melbourne Telescope as it celebrates its 150th anniversary.
Play award winning games by Australia's emerging designers in the Australian STEM Video Game Challenge
Spend an Evening with Alice Bowman, NASA New Horizons Mission Operations Manager, in conversation with Emmy award-winning journalist Sara James.
Discover the power of LEGO® Mindstorms® EV3 robots and coding in this introductory workshop.
Make and create with Makey Makey® in this introductory workshop.
Discovering imperceptible worlds through science and technology.
Get ready to build your curiosity! An exhibition for babies to 5-year-olds.
We're inviting you, our Museum Members, to visit and peruse Scienceworks shop in an exclusive morning shopping.
Explore the remarkable properties and mysteries of lightning.
Enjoy a day of fun and play-based learning activities without the school groups!
What will the future be like?
Stretch your mind and your muscles.
A tour filled with stories of old Melbourne and Victorian engineering feats.
See highlights from our historic collection and discover some of Melbourne's fascinating past.
Join Jack Larson on a wild ride through our Solar System as he searches for a new world to call home.
Meet Tycho, a dog who doesn't just howl at the Moon, but wants to go there.
Imagine a place where time stands still and the known laws of physics don't apply.
Uncover the mysteries of the stars in this Planetarium show.
When the seasons go crazy Annie and Max must set things right and discover a new angle on the world.
Take a trip to the far reaches of space, with our Scienceworks presenters as your tour guide.
All-sky astronomy is a new way of looking at the sky to better understand our Universe – and it's happening in Australia!
A new way – the oldest way – of looking at the night sky.
When is a planet not a planet?
A dramatic and different way of looking at our climate.