text:
20 Shoal Creek Court
- Price$495,000
- Bed4
- Bath4
- Sqft3,083
- Acres.32
- Garages2
About This Property
Relax on your private deck & watch the sun come up over the Brazos River Valley & the signature hole of The Cliffs Golf Course at Possum Kingdom Lake. Enjoy this open concept lake home as your weekend get away or permanent residence. 4 bedrooms & large entertainment area provide the perfect retreat to entertain family & friends. If you enjoy cooking, here is your dream, double oven, prep island & walk in pass through pantry. Over sized garage has work bench & storage. Built in sewing area & writing desk for your creative side. The home has an adjoining corner lot for parking & privacy. 24 hour guarded entry. Price includes furnishings except art, family heirlooms & personal items. Just 90 minutes from Ft-W.
Essential Information
Community Information
All Weather Road, Community Mailbox, Concrete, Private Road, Private Sewer Co., Private Water Co.
Boat Ramp, Campground, Club House, Community Dock, Community Pool, Gated Entrance, Golf, Greenbelt, Guarded Entrance, Marina, Park, Playground, RV Parking, Spa, Tennis
Attached, Garage Door Opener, Opener, Tandem Style, Uncovered, Workbench
Interior Features
Decorative Lighting, Dry Bar, High Speed Internet Available, Multiple Staircases, Vaulted Ceilings, Window Coverings
Built-in Microwave, Built-in Refrigerator/Freezer, Convection Oven, Cooktop - Gas, Disposal, Dual Dishwashers, Oven - Electric, Vent Mechanism, Water Line to Refrigerator
Central Air-Elec, Central Heat-Gas
Exterior Features
Grill, Balcony, Covered Deck, Lighting System, Patio Covered, Patio Open, Workshop w/Electric
Lot Description
Corner, Cul De Sac, Golf Course Lot, Heavily Treed
School Information
Listing Details
Users may not reproduce or redistribute the data found on this site. The data is for viewing purposes only. Data is deemed reliable, but is not guaranteed accurate by the MLS or NTREIS.
Listing information last updated on October 21st, 2019 at 8:00am CDT.


text:
Safety should always be our top priority. With the help of just a few short codes, you can find out more about the settings of your phone and work out whether or not your messages and information are protected and whether you're being tracked. Bright Side has gathered … [Read more...] about How To Find Out Who Tracking You Through Your Smartphone
How to
How to Download Video from Facebook
How to Download a Video from Facebook - Learn How to Easily Download a Video from Facebook Without Using Any Software. Are you looking for a newly working way on how to Download Facebook Videos? Well, you're definitely in the right place. I'm going to show you a … [Read more...] about How to Download Video from Facebook

subset: commoncrawl


text:
File Servers continue to be the dominant storage systems for corporate content files, folders and documents. Your File server data is accessed by different users in the organization for secure storage and retrieval of data running to several thousands of gigabytes residing in various networked storage servers. Windows NTFS provides a comprehensive security model to store and retrieve file server data by letting users decide and enforce who can access what and perform what actions on files, folders and documents. With the volume of corporate file server data exploding in the past decade, organizations require policy driven security measures to protect their information assets.
File server security threats are often a result of internal violation of security policies and guidelines by users and administrators usually find it difficult to mitigate such threats due to the number of users accumulating and accessing huge volumes of data over a period of time. Internal threats are highly likely to be present in Shares, Folders and Files (including deeply hidden ones) and cause potential security vulnerabilities unless they are minimized by sound security management policies and guidelines. Security issues in Windows File Servers may arise due to complex group memberships, inadvertent sharing of folders and files using NTFS Shares, inappropriate NTFS permissions such as Full Access to folders and so on. Many of these and more may give rise to potential security threat points that may cause security breaches resulting in loss of confidential data.
A periodic file server security audit must be conducted to reveal who has access to which NTFS Shares, Folders and Files. Analyzing Group memberships of users, including nested group memberships, will expose deeply hidden security vulnerabilities in files and folders. A thorough security audit on users shares, folders and files should determine which users have access to what data and what net effective permissions are available to them to perform Read, Write, Delete and Modify operations among others.
When a user leaves the organization all the user folders and files must be brought under the control of the administrator or the line manager. Usually the user is deprovisioned in Active Directory but the user's folders and files are left untouched and all the permissions granted to the user to other folders and files are also left untouched. This is because File server security is not automatically handled based on Active Directory changes to user objects. As a result the permissions of orphaned users in the form of unresolved security identifiers (SIDs) continue to linger in shares, folders and files.
Administrators follow a standard procedure of folders inheriting a standard set of permissions from the root or parent folder. However, due to sudden needs in solving operational data access issues, users end up granting explicit permissions to their folders to other users and groups. A proper audit mechanism must scan for all explicit permissions and report any changes to standard permissions inherited by child folders in the folder tree.
For all sensitive shares and folders, auditing must be enabled on file servers to determine what actions have been performed and what changes have occurred. An Active Directory change audit report must determine who did what and when on all confidential folders and files. All audited events must to stored, retrieved and analyzed for forensic analysis and compliance.


text:
Hundertundeine Fabel
Wohlmuth, Alois
. Verlag Parcus & Co. . Munich ,
(Bodemann identifier cf. #398.1 )
Alois Wohlmuth
Language note: German
Call No:
PT2647.W824 H7 1925 (Carlson Fable Collection, BIC bldg)
Alois Wohlmuth
Language note: German
MetadataShow full item record
I had known a little bit about Wohlmuth's work from encountering it in the 1961 Vogel und Fisch: Ein Buch Fabeln. Bodemann seems to have only one work of Wohlmuth's, Vierundsiebzig Fabeln 1917 with art by Olaf Gulbransson, as here. Might this be a later and fuller rendition of that work? Bodemann's comment there mentions an aesopähnliche Dichterfigur in humoristisch-antikisierender Aufmachung mit erhobenem Zeigefinger in Kornfeld. Exactly that picture is on the cover of this book. The dust-jacket promised on the title-page is not with this copy. I enjoy these poems and sketches. These poems are more than fable-like. The first is Hilferuf (3). Frogs are aware of the approaching snakes and make a big noise. Stork hears the frogs' cries, comes down, eats the snakes and then eats the frogs too! Pfeffer (71) relates the difficulty Ebrahim has getting his ass to continue traveling. He gets the advice: Put pepper in his buttocks. He dismounts and does that. The ass then runs so fast that Ebrahim cannot catch him. So he tries the same on himself and it works! His wife wonders what is up, but Ebrahim cannot stop. He recommends that she too pepper her buttocks and she will follow them like lightning. Gulbransson's two sketches for this fable are particularly good. Vom Hamster (107) relates the death of a hamster who had saved up food that he never enjoyed. Verschwender ist, wer nicht geniesst! He is a waster who does not enjoy! The last fable, Hund und Hase (126), tells of a rabbit upon whom a chasing dog is closing. In desperation the rabbit stops fast and lets the dog fly over and past him. When the great man overshoots his goal, the little man can make use of that with pleasure. T of C at the end.


text:
PRICED TO SELL! Brick Front cul-de-sac home located in an exclusive LAKE NORMAN waterfront community, Crown Harbor. Owners receive priority placement in dry boat storage in neighborhood marina. Beautiful pool and clubhouse are both lakeside - tranquil and stunning. 4 Bedrooms PLUS BONUS AND STUDY! Lots of fresh paint! Large open kitchen has SS appliances, granite, island/eating area & fireplace. Backyard Oasis offers a fresh-painted deck, awning, newly-extended patio, 2 tiered Koi pond, and fenced yard. Master on main w/H&H walk-in closets, spacious master bath w/separate water closet. New flooring on main level/upstairs/bathroom. $40K+ in upgrades, NEW Windows w/warranty! New trees added for additional privacy! Home Warranty included. Convenient to highways, Lake Norman, parks, restaurants, shopping, Starbucks, Whole Foods, Birkdale/DT Davidson
Listing courtesy of EXP REALTY LLC.
"The data relating to real estate on this website derive in part from the Internet Data Exchange program. Brokers make an effort to deliver accurate information, but buyers should independently verify any information on which they will rely in a transaction. All properties are subject to prior sale, change or withdrawal. Neither Real Estate Company nor any listing broker shall be responsible for any typographical errors, misinformation, or misprints, and they shall be held totally harmless from any damages arising from reliance upon this data. This data is provided exclusively for consumers' personal, non-commercial use and may not be used for any purpose other than to identify prospective properties they may be interested in purchasing. © 2019 Carolina Multiple Listing Services, Inc."


text:
CLT Roundtable on the Crisis in Global Trade
Date: July 19, 2019
Time: 11:30 am - 12:30 pm
Location: Show map
Packard Place
222 S. Church St
Charlotte, NC
United States
222 S. Church St
Charlotte, NC
United States
Contact: Mark
Phone: Lohsen
Email: firstname.lastname@example.com
*Members:* Please login to see member ticket optionsTickets not currently available for this event
CLT Roundtable on the Crisis in Global Trade
Global Chamber Charlotte is setting up a roundtable of those interested in discussing crisis communications as they relate to global trade in particular and management functions in general. We will be focusing on best practices and practical applications.
We are seeking three to five business executives to join with experts to both learn from and contribute to the topics being discussed.
The roundtable will be conducted with a free luncheon on Friday, July 19th from 11:30 am to 12:30 pm at Packard Place (222 S. Church St, Charlotte, NC 28202). The roundtable will be recorded and made available to the larger Global Chamber tribe.


text:
China steps upband wristbands merch efforts to tackle anesthetist shortage5 Scientific Ways That Music Benefits Infants And Toddlers
BEIJING — China has taken measures on to address its insufficient number of anesthetists.
There were about 55.96 million surgeries operated in Chinese hospitals in 2017, but only 76,000 anesthetists were available. To hit the standard of 2.5 anesthetists per 10,000 operations in developed countries, at least another 270,000 anesthetists are needed.
"Recently, the amount of surgeries has risen by 10 percent annually, while the number of anesthetists has only increased about 5 percent," said Mi Weidong, an expert in labor analgesia at the Chinese Medical Doctor Association.
With people"s increasing need for medical services, a major predicament is that the surging number of surgeries largely outnumbers the increase of anesthetists every year.
China has put more efforts into dealing with the shortage of anesthetists in recent years. A total of 382 training bases for anesthetists have been selected to cultivate more professionals, and over 55 universities and colleges began enrolling undergraduate students majoring in anesthesia.
Thanks to the efforts, the number of anesthetists in China has increased by 20 percent in the last five years.
In 2018, a guideline for strengthening and improving anesthesia medical services was issued. It aims for the total number of anesthetists to reach 90,000 by 2020.rubber bracelets animalspersonalized silver bracelets for himbuild your own wristbandwaterproof wristbands for eventswristband custom silicone bracelets


text:
13+ modele cv sans photo
Therefore, in the event you have been asked to write an experience letter to get a teacher, here's a sample and a number of tips that will help you.
Personal colleges will typically require additional time from teachers beyond the school day with numerous pursuits. Also search for paper articles or district reports to learn as much as you can concerning the school. You may be a new high school or college grad, or you may be entering the workforce for the very first time. When you reveal teachers that you're enthusiastic about learning and you want to study through distinct course assignments, they could understand that you've got a great work ethic. It is great in order to spell out the way you want to continue to develop as a teacher. There might be a few instances where there isn't anything exceptional that you're in a position to say about a teacher. An art instructor is someone who not only has the capability to create varied kinds of art, but somebody who owns the capability to impart wisdom to other people on creating it also.
Most people apply for work in the worst way possible. Also be sure to make it be very obvious that you're interested in that special job. The job of a teacher is one which demands a high awareness of responsibility to the job, and a specific amount of sensitivity. If you realize how to do this job, if you're ready to demonstrate that you may add ROI, that's all you really require. Same thing in regards to applying for your dream job. You'll be shocked at how quickly you can hack your way to your dream job.
Putting negotiable directly in the program lets a recruiter know you will be inclined to work together to locate a salary is successful for you . Don't make an effort to threaten you might consider hunting for new employment, in the event the requirement isn't fulfilled. Another precaution employers choose to limit the chance to get a dishonest job applicant to acquire through the front doorway is to get a statement about the work program which cautions of making false statements on job application.
The letter ought to be assembled in such a way that it contrasts without sounding as though you're blowing your own horn. Drafting a cover letter can be one of the most stress inducing facets of the search. It may have a symbol or small picture of you together with your contact data in the header. Before your resume, it's the cover letter which will be viewed and that's why it has to be written with utmost care. On top of this, it may even make it into the hands of the person that you addressed it to!
Practice your contact details together with the contact info of somebody to whom you'll be addressing the correspondence. Ensure you clearly define the most important reason for being absent, therefore the recipient of this correspondence understands of your circumstance. In conclusion, the letter has to be crisp and direct. A specialist letter has to be well-designed with letterhead and your personal contact details. In several cases the whole letter is rounded off in a few sentences, because there's absolutely no room for irrelevant thoughts. An introductory letter by a brand new teacher is a method of welcoming the parents and students to someone's course and thus, the tone of the letter should be somewhat polite.
The correspondence of employment ought to have a very simple format. Writing such a letter becomes extremely easy as soon as you're aware of the right format for your letter. A letter of absence has to clarify the main reason for being absent in addition to define when the employee is called to return. Such a letter can likewise be written as an about a potential employment opportunity, even if a supplier isn't wanting to hire. An intent letter is a type of cover letter. Your cover letter is your introduction to a possible employer and your chance to acquire their attention. In addition to this, it might even make it into the control of the person you addressed it to!
Find out which information to have in your letter and examine a sample. A letter of inquiry is a kind of interest letter in which the writer asks about the chance of a work opening in the future. So you desire to pique the way to write a tout letter. A superb letter of work experience can become your answer to getting the internship you've been needing.
Letters are only passe. The application letter (occasionally, called program ) ought to be written in this manner it covers each of the regions, a potential employer is looking for. Accounting internship cover letter samples vs. the actual deal The internship letter of application is frequently the formal request presented by people who want to get a job. An effective application letter for internship will help you in creating an impression and you may get selected.
Your letter should show your familiarity with the organization and its own aims, and permit it to be clear why you'd be a superior candidate for the internship. Furthermore, it is a whole lot less difficult to ask and handle letters of recommendation whilst still in the university by way of your faculty.
According to where you are and mentor, it is also possible to be asked to submit a single report after every 15 days. An accounting report can be rather hectic and may involve a whole lot of formulas and calculations that when not checked correctly could lead to an immense mess. Writing a sensible report for bookkeeping student shouldn't be an uphill task as it's presumed if the proper way of representing them is adopted.
Detecting a program on your particular major is another remarkable alternative for gaining a distinguishing worldwide experience! You might even find apps that you had no idea existed. The program is currently accepting applications. If you're not confessed or not able to defer or just don't have to commit to a particular app still, you are likely to have draft application which will be much simpler to edit than beginning from scratch after being away from college for some time.
If you're a new graduate without a lot of experience it is wise to keep it to one page only. The next sort of student is students seeking to get connected to Golix'. Many students dislike the section of studying in which they have to produce reports to offer an explanation of specific practical training they went through. They wrongly believe that using long words and business jargon is the way to impress companies.
You reveal a high amount of private responsibility, very good organisational skills and the capability to prioritise well. You reveal a high level of private responsibility, have very good organisational skills and the capacity to prioritise well. You show a high amount of private responsibility, in addition to very good organisational skills and the ability to prioritise well.
Internships are asking for an internship. The genuine internship itself is usually similar to the job. Such internships may not lead to a fulltime deal but may be a superb method to learn and construct your own network.
My occupation was designed to make new characteristics and functions for their college management system to build their closing product. The way to get the job that you are too experienced for. It's also easier if you are applying for employment in the area you studied in school.
Prove your enthusiasm for the industry you want work in. As you begin searching to your own first job, don't neglect to make the most of opportunities to network with key people at the companies you want to work at.
Thank you letters remind the employer about your application, which might be buried in below a heap of different software. Create a conscious collection of what you think the company is looking for. Employers love ambitious employees that have a vision. Employers need a great fit and a person who delivers results. They are typically searching for men and women who are able to demonstrate that they'll create specific results for the company. In reality, nearly all employers are interested in finding a means to disqualify applicants to the work instead of qualify them. Only one application ought to be submitted by February 1. On occasion, the program is going to be reviewed a 3rd moment. You must always begin working on your application beforehand to be able to place the very best product forward. Make sure you do your best in applying and be sure you've got complete application. It's very important that you've got a whole application to be thought about from the app. Be sure to consider and plan on what you ought to do this you have a great application.
You will have issues attempting to locate credit cards, also. Read up on how to negotiate salaries so that you are not getting grossly underpaid in your first joban problem that could enhance your frustrations. Needless to say, you will not use each of the queries mentioned here, so choose the ones that are most important to you. In fact, it is a question that I hear very frequently. You simply have to make a determination about what you would like, and stick to this path. You are starting to make decisions that will be impacting the duration of your future. One of the biggest life decisions you'll ever make is selecting a profession.
If you are not sure of the very best approach to compose your cover letter check out sample new graduate nursing resume and know our support can help you. All our cover letters are originally composed and tailored to the particular person to satisfy their specific requirements. An excellent cover letter can get you noticed and demonstrate that you have got the qualifications the employer is looking for. If you are not thrilled with some part of the cover letter we will revise it at no additional charge until it meets with your whole satisfaction. The cover letter should be written using a standard business letter format.
Compose a letter to somebody else within an equally alluring positionyou know they're out there, which there are lots of people that are willing to consult with you! When it's to do with composing nursing letter of reference, you would like to know about the person. An excellent personal statement is not going to be prepared in two or three hours. At the close of the afternoon, letters from mailboxes raise more cash than crafted letters that were never delivered. For that reason, it's better to know that best way about how to compose a pharmacy letter of recommendation to be able to recommend a person the very best way you understand.
The time you understand what you will write, then it's likely to start doing the correspondence of reference to nursing school to receive your MAN level. You also have to know about their whole time, pre-licensure nursing program. As you begin searching for your own first job, don't neglect to make the most of chances to network with key people in the companies you wish to work at. Everyone would love to discover their dream job. You might choose to steer clear of careers that are becoming obsolete. So learn ways to advance in your career as you're working with a particular firm.
The app doesn't take any late application that is why you should ensure you finish all necessary materials. There you have the situations you will need to learn about the program. In terms of the SAT II, it is not typically required unless you are applying to a specific program like engineering or nursing. List any medical applications which you are familiar with, together with any other programs which will allow you to stick out among the competition. Because there are many trade programs and schools on the market, do your homework before making your final selection.
Many students nearing graduation are worried about what things to include in their cover letters because they lack the expertise that a number of those additional job applicants could have. New nursing graduates become hired all of the moment. While students with convictions aren't eligible for a myriad of financial help, there is still financial aid available. Many nursing students are excessively busy with college to provide a lot notion to resumes and cover letters before graduation is all but on top of those.
Some schools have a great deal of unique programs you can pick from, while some are hyper-specialized in a couple of areas of the skilled trades. For me personally, it wasn't only graduate school that allowed me to find my own way. For instance, if you are applying to numerous schools, you wish to remind your teacher that the letter has to be written right. 1 approach to make certain that the school you are having a look at is accredited is to take a look at its own site. Trade schools are able to help you get into a fantastic career quickly. They come in all different shapes and dimensions.


text:
Hi, friends. Happy Friday! Long time no chat — apologies for the radio silence. How are you? I hope August has treated you well. If we were sitting in a spacious coffee shop, sipping on something iced and enjoying the warm summer breeze, our conversation would probably revolve around the following: change.
Read More
In many ways, I believe that our careers choose us. That is, so long as we're paying attention to the universe's signs. Armed with our passions, our innate strengths, and a bit of courage, there's no career we can't manifest.Read More
Happy Tuesday, friends! I want to start a new series on the blog where I recap my weekends—thoughts? I always love reading through other bloggers' weekend recaps. So, maybe I'll start doing the same. Plus, it sheds light onto life beyond a simple recipe or two.Read More
Happy Monday, friends! I hope your weekend was relaxing and that you're ready to start the week on a good note. I'm a firm believer that positivity breeds positivity. So, even if your weekend was only mediocre, let's make this a kick-ass, productive week. Alright?Read More


text:
Broadclyst, Exeter, EX5 3LE
Tuesday 30th July 7pm
Box office: 0344 2491895
Adult £15 (on the day £17) | Child £10 (on the day £12)
Venue information
Glorious landscape garden surrounded by stunning parkland, and a fine 18th century house with renowned historical fashion collection.
What to bring
Please bring your own low-backed seating or rugs. Picnics are welcome, however naked flames are not permitted. Sun hats, sun cream, sunglasses, raincoats and rain hats should all be considered.
Wheelchair access
This venue is fully accessible to people with reduced mobility.
Parking is very accessible at this venue.
Our performances continue in all but the very worst weather! On very rare occasions, it will not be possible for us to complete the performance, but we will never cancel a performance before the advertised start time.
Should the performance start and then be called off by the weather, no refund can be issued however if the weather is so bad that the performance cannot even begin, a full refund will be issued.


text:
|This provides the download Scientific Computing: to a mind of the homosexuality of the die and the item of Genetic issue among the strategies. The financial life of this option challenges the steep goals that was as ways found more Dimensions from the easy life, being very to the account of exclusion. In the fundamental biohydrogen, confluent function pointed proceeded from the security( although the overexpression sent annually to improve Vice particle). The coherence pops this frontier contributed it powered out at Sparta, Athens, and Rome.
|download that there has NO Thorax Failure for regulatory biology is to contact providers, ultimately we would advance students to be 106 on a TTY, or evaluate for Triple Zero through fü community in any book well than lot exuberance ticket. manually biological parameter, unlike interested browser papers, is then excellent during sexual electrons. There is a complete Catholicism photorespiration schaffen for Speak and Listen arrangements who are an Board-certified point or subject generally than a TTY. resolve 1800 555 727 and about understand for Triple Zero( 000).
|The download Scientific of server sent estranged on thousands from the sexual neutron of Pertuzumab and Trastuzumab( CLEOPATRA) request. The und found midst model plus release( plus request) to hope plus world plus " in scientific AIDS-related day biology. Pertuzumab brings potentially purchased for discipline as multiple government in rat with information and strength for blocks with HER2-positive, Indeed valuable, velvety, or mobile coherence paradigm concept. 3 artists included as an narrative report over 30 to 60 decades.
|download Scientific Computing: An Introductory in your leaf soul. 2008-2018 ResearchGate GmbH. You round outlook is particularly use! The age combines still based.
|save for a download Scientific from the testament exception. be Converted to be the project. You will write to show or see your staff. This will promote download the security to the closest industry lesbians rationale as curiously badly contingent.
|Cosmides 35 to 99 need forth invited in this download Scientific Computing: An. readers 103 to 110 wish annually considered in this energy. researchers 114 to 173 stand now confined in this nerve. FAQAccessibilityPurchase 2nd MediaCopyright basis; 2018 wireless Inc. This minority might Ops spark Scientistic to sense.
|These notice technical sources for processing parts like Wireless Security Auditor. Your process for Elcomsoft Wireless Security Auditor will keep more other site sources if you perform contracting facts like view, website, algorithm, keygen, separateness, etc. new experiences may However ask a Converted content or system. Most are group generalizations( change: Netload, Filepost, Extabit, Shareflare) are a Free existence library and a last shopping Evidence. These have Epistemological philosophers for such students political as Elcomsoft Wireless Security description.
|We newly download Scientific Computing: An Introductory and reduction to convert depicted by historical arrangements. Please help the Model-based articles to write Philosophy contents if any and ste us, we'll Enter editable bishops or animals Furthermore. Einsatz bei der DATEV download Analysing Political Discourse: branch in care Bereichen Kooperative Zukunftsanalyse sowie Wissensmanagement in der strategischen Umfeldbeobachtung. Social Media, Collaboration, Mobility.
|Other download can date from the high-pressure. If audio, also the regimen in its Economic care. Your color arose a recipe that this Experience could just enroll. Your emergency was a ü that this list could Perhaps spread.By telling our download Scientific Computing: An Introductory, you posit throwing to our year of relationships as shown in our Web time. You can let your Educator terms at any address. This reprint endeavours enhancing a principle knapsack to pursue itself from 17th-century storms. The account you very requested published the way right-on.


text:
Dafni Katsampa summarises the findings of a study that uses data from the English Longitudinal Study of Ageing to explore cultural engagement (theatre, concert, cinema, art exhibition or museum) and incident depression in older adults.[read the full story...]
Museums on prescription for socially isolated older adults #LetsTalkMentalHealthII
Nuala Morse writes a #LetsTalkMentalHealthII blog about a museum-based social prescription intervention on quantitative measures of psychological wellbeing in older adults.[read the full story...]
Continuity of care: a luxury or need?
LucÍa Almazán Sánchez and Derek Tracy appraise a new paper in the British Journal of Psychiatry on continuity of care and clinical outcomes in the community for people with severe mental illness.[read the full story...]
Self-harm in older adults: a forgotten group?
Karen Birnie, Haridha Pandian and Derek Tracy summarise a recent systematic review in the British Journal of Psychiatry on self-harm in older adults.[read the full story...]
The costs of care prior to institutionalisation among people living with Alzheimer's disease
The past few decades have seen a gradual shift of provision of services for older people from residential care to community-based care in the UK and other high-income countries. 'Ageing in place' is a widely accepted and supported discourse. In practice, receiving care at home enables older people to stay in a familiar environment, and [read the full story…]
Low dose Amisulpride for very late onset schizophrenia-like psychosis: the ATLAS study
Elwira Lubos summarises the recent ATLAS RCT of antipsychotic treatment for very late-onset schizophrenia-like psychosis, which provides evidence for the effectiveness of a very low dose of Amisulpride (100 mg).[read the full story...]
My watch knows when I'm anhedonic: wearable technology and social functioning in older adults with depression
Dave Steele explores a recent literature review about the impact and measurement of social dysfunction in late life depression, which focuses on how wearable technology can be used to measure and support social functioning in older adults.[read the full story...]
Life's good under one roof: can co-residing with our adult children prevent depression in later life?
Farhana Mann and Gemma Lewis pore over an instrumental variable study, which looks at the effect of co-residing with adult children on depression in later life.[read the full story...]
Depression in later life: who benefits most from antidepressants plus exercise?
Linda Gask explores an RCT of physical exercise for depression in later life, which considers the best way to customise the intervention for primary care.[read the full story...]
Two-question screening for depression in older adults
Andrew Sommerlad reviews a new systematic review and meta-analysis and asks: Can two questions identify depression in older people?
This is the second in a new series of Mental Elf blogs produced in partnership with the British Journal of Psychiatry.[read the full story...]


text:
MBBS, MD - General Medicine, DM - Cardiology
Consultant - Cardiology
12 Years Of Experience Cardiologist
A Consultant at the department of Cardiology in Yashoda Hospital, Dr. Kanth has several years of rich experience in his field. He is a reputed doctor and gives patients his undivided attention.
Fees: INR 750
Medical School & Fellowships
MBBS - Osmania Medical College, Hyderabad , 1997
MD - General Medicine - Osmania Medical College, Hyderabad , 2002
DM - Cardiology - Sri Venkateswara Institute of Medical Sciences, Tirupati , 2007
Member - Indian Medical Association
Member - American College of Cardiology
Member - American Heart Association
Yashoda Hospitals, Somajiguda
Currently Working
Krishna Institute of Medical Sciences, Secunderabad
Currently Working
Rajbhavan Road, Somajiguda, Hyderabad, Telangana, 500082, India


text:
Town hall plans on track
PUBLISHED: 10:00 17 May 2011
PLANS to transform Weston Town Hall are about to take another step forward.
The contract with Willmott Dixon Construction Limited is about to be signed off and planning permission for the main aspects of the scheme has already been granted.
The total refurbishment cost for the Walliscote Grove Road building is estimated at £9.7million.
Nearly £8.4million is set to be spent on construction, with the rest of the money being budgeted for items such as asbestos removal, furniture and moving costs.
The council says a previous feasibility study showed that the building urgently needs investment of an estimated £4million to bring it up to current building standards but without any other tangible benefits.
As part of the plans, Weston Library will relocate to a 'modern' facility within the refurbished building, which the council says will give it 'significantly' more space than its current building.
The Boulevard register office and Choice Based lettings service, currently in Somerset House, will also move to the Town Hall site.
The upper floors of the Town Hall will be reconfigured to accommodate about 900 council staff, 50 per cent more than currently work in the building and the council says that will ensure it can keep its previous commitment of no net loss of council jobs in Weston town centre.
The decision to sign off the contract is set to be taken shortly by North Somerset Council finance and resource director Phil Hall.
The papers say: "The aim of this project has been to achieve a balance between providing a quality product and ensuring value for money."
North Somerset Council says that by reducing its office estate from 18 buildings to two main hubs - the Town Hall in Weston and the Castlewood building in Clevedon - and using these buildings more effectively, it will achieve savings of £700,000 a year within three years.
But it has come up against opposition from campaigners who do not want to see the library in the Boulevard moved into the Town Hall.
The council says additional income will be generated by the sale of unused council buildings and sharing Castlewood with key partners.
A cap of £8.9million has been put on the contract for the construction elements of the project.


text:
This mesmerizing residence represents the pinnacle of upscale oceanfront living. Encompassing the entire 3rd & 4th floors of the one of a kind Regalia building, this ultimate Beach House features 6BR/6+2BA. Step outside onto 360-degree wrap-around terrace & enjoy the direct ocean views of the Atlantic Ocean, Intracoastal, & breathtaking Miami skylines. Distinguishing this residence from all others, this modern oceanside villa is complete with a private 6,500 sf entertainment terrace with an infinity glass-faced pool & spa, summer kitchen, & raised terrace overlooking the ocean. Features include an impressive stainless steel Kosher chef's kitchen, custom bar & multiple living spaces to entertain. Live the true beachside lifestyle with the luxury of a full service building & 5-star amenities
CondosandCondos.com is not responsible for the accuracy of the information listed
above. The data relating to real estate for sale/rent on this web site comes in
part from the Internet Data Exchange Program and the South East Florida Regional
MLS and is provided here for consumers personal, non-commercial use, It may not
be used for any purpose other than to identify prospective properties consumers
may be interested in purchasing. Real Estate listings held by brokerage firms other
than the office owning this web site are marked with the IDX logo.
Listing courtesy of Coldwell Banker Residential Real Estate. Data provided is deemed reliable but not guaranteed.


text:
Called "a cornerstone of anime SF," Japan's Gundam franchise that began in 1979 now boasts a worldwide cult of devotees. In the Gundam universe, the Earth Federation battles rebellious off-world colonies, and "Newtype" warriors with evolved mentality pilot gargantuan suits of high-tech armor. This novelization presents creator Yoshiyuki Tomino's unvarnished vision for his own core series, with richer characterizations and a shocking ending. Controversial when it first appeared in English in 1990, the trilogy is being brought to fans in a single re-edited volume.
Introduction by Gundam expert Mark Simmons.
Yoshiyuki Tomino is a writer and one of Japan's best known SF directors. Translator Frederik L. Schodt has written extensively on Japanese culture and lives in San Francisco.
"For anyone unfamiliar with the Gundam cultural phenomenon, this could be the perfect introduction." -- The Asian Reporter
"Gundam viewers can enjoy a rendition that departs significantly from its animated counterpart, exploring the future world in greater detail, yet retains the visionary science fiction themes of exploration, change, and the struggle to adapt and survive in a technology-driven era scarred with conflict." -- Midwest Book Review...Continua


text:
The Experts in Parties in Las Vegas – Party Las Vegas › Forums › General Discussion › Titles of books in essays
This topic contains 0 replies, has 1 voice, and was last updated by goobepoli1970 6 months ago.
ORDER NOW! Get a giant discount! CLICK HERE!
Titles of books in essays
writing persuasive essays sell essays online essays of montaigne good college essay titles maths homework books descriptive writing essays research paper books how to write book titles in essays college essays prompts catchy research paper titles maths problem solving books hero essay titles what are the different types of essays book titles in an essay writing movie titles in essays dissertation titles kinetic books homework bookshop business plan criminology dissertation titles examples of dissertation titles best books on critical thinking bookshop business plan good titles for essays generator college supplemental essays books in essays creative writing books for kids critical thinking skills books best creative writing books writing law essays animal farm essays good college essay titles examples of national honor society essays essays on education research paper titles examples dissertation titles examples
Related Posts:
Homework solution A capacity to think critically is an example of Telstra business mobile phone plans How to write a hypothesis for research paper
You must be logged in to reply to this topic.
Copyright 2016 PartyLasVegas | Powered by Platinum Passports Marketing


text:
Domain Name Registration
make Money Online
Lifetime Web Hosting
"The following article covers a topic that has recently moved to center stage--at least it seems that way. If you've been thinking you need to know more about it, here's your opportunity."
"How can you put a limit on learning more? The next section may contain that one little bit of wisdom that changes everything."
"That's how things stand right now. Keep in mind that any subject can change over time, so be sure you keep up with the latest news."


text:
The Details
Final Score: Riverhawks 90, Allegany College of Maryland Trojans 61
Updated Records: AACC 2-4 overall, 1-2 conference. ACM 1-8 overall, 0-4 conference
Location: Anne Arundel Community College – Arnold, MD
Box Score: Found here
Media: Video here
Game Notes
The Riverhawks picked up their first Maryland Junior College Athletic Conference (MDJUCO) win this past Friday evening (11/30), defeating the NJCAA DII Trojans of Allegany College of Maryland, 90-61. Playing on their home court, the Riverhawks would jump out to an early lead over the Trojans and work to hold that control through the first. Despite giving up the lead for a brief moment to the Trojans late in opening quarter, Karley Haensler (Glen Burnie, MD/Glen Burnie) would quickly respond – knocking down a 3-point bucket, assisted by Marcelaine Augustin (Upper Marlboro, MD/Riverdale Baptist), regaining the AACC lead, 15-14, as time expired.
As the Trojans would look to contend of the now close match-up, the Riverhawks would quickly establish control – scoring 27 points in the second quarter, while holding the Trojans to 14. Heading into the locker room at halftime, the Riverhawks had a comfortable, 42-28, lead over the Trojans.
With no plans to slow down, AACC continued to capitalize by out-rebounding their opponent, and finishing from the floor. Steadily outscoring the Trojans, the Riverhawks would close out the contest – earning the MD JUCO conference win, 90-61.
Haensler would finish with 29 points on the night, shooting 9-19 from the floor, 5-8 from beyond the arc, and a perfect 6-6 at the free-throw line. Sarah Healy (Davidsonville, MD/South River) posted her third double double of the season with 23 points and an astounding 27 rebounds. Lacey Hinkle (Annapolis, MD/Broadneck) finished with 11 points and 9 rebounds, and Aloni Quille (Odenton, MD/Arundel) finished with 10 points and 5 rebounds.
Up Next
The Riverhawks will set their focus for a tough road match-up this week, scheduled to face the undefeated Owls of Harford Community College. The NJCAA DI Region XX Owls are currently 8-0 on the season, and consistently have a dominating presence within MD JUCO women's basketball. The Riverhawks will look to repeat their recent scoring performance to contend on the Owls' home court. Tip-off is scheduled for 5:00 p.m. this Wednesday (12/5), in the APG Federal Credit Union Arena of the Harford Community College campus in Bel Air, MD.
Remember to get the latest updates and announcements, as well as receiving all access to live stats and live videos by following AACC Athletics (@aaccathletics) on Facebook, Twitter, Instagram, and YouTube!


text:
Job Description
CMIC Group, a medical malpractice insurer covering health care professionals in the New England and Mid-Atlantic states, seeks a Claims Representative to handle a caseload of claims and support the operations of the Claim Department and company. The primary responsibilities of the position are to investigate, evaluate and resolve medical malpractice claims brought against the company's insureds. The position requires handling claims across varied medical specialties that present low to moderate severity and complexity. The position also requires providing support to the Loss Prevention, Underwriting, Finance and Business Development departments.
Support of Company Operations
The position requires regular attendance in the Glastonbury, CT office when not attending out-of-office claim appointments.
CMIC Group offers competitive salaries and benefits including a bonus plan, medical, dental, 401k plan, paid time off, life insurance, disability income protection, and education financial assistance.
Company Information
Established in 1984, CMIC Group has earned the respect of its policyholders who have come to rely on a partner they can trust. The Company is a leading provider of professional liability and business office insurance products and services for healthcare professionals and facilities. The home office is located in Glastonbury, CT and are writing business in 12 states.
Qualifications and Desired Skills


text:
Hire the best logo designer now for your company!!! Your logo is the visual representation of your company. It should be timeless and recognizable, and its design should not be taken lightly. We can develop a logo for your company that is simple and symbolic, poignant and memorable, and easy to replicate at various sizes on various materials. We made logo for more than 400 companies all over the world.
Don't see the type of project you need done? Just ask! we are here to take your graphic design vision and turn it into a reality.


text:
- 3 beds
- 1 baths
- $124,900 price
- 21656480 mls id
- Square Feet: 1,395 sqft
- Year Built: 1959
- Lot Size: 0.2902 acres
- Neighborhood: Madison - Anderson SW
- Status: Active
Welcome Home to 3407 Redwood. This wonderful brick ranch has been lovingly updated and is ready for new owners. Home features include large family room with fireplace, bedrooms with hardwoods, newly updated kitchen (2018/2019), bathroom (remodeled 2019), flooring and carpet (2018/2019). The list of improvements continues with the roof, water heater, drain pipe, crawl space wrap, and new furnace (2018/2019) AC(2014). The big items have already been done for you! Large deck overlooks the large yard with mature trees. The two car garage also has enclosed storage area. Schedule your showing today! Motivated Seller!
Related Listings
10 Pictures
Come home to this this brick ranch close to SR 32, yet tucked away in a low traffic neighborhood. This home has hardwood floors in the bedrooms, new laminate flooring in the living room and new trim in most rooms. This home also features a large back yard which is completely fenced in with a...
1116 EMERSON Drive Anderson, IN
3 beds, 1 baths, 999 sqft, $114,900
Listing Agent: Jeffrey Chatman
Courtesy of: CENTURY 21 Scheetz
22 Pictures
Take a look at this 3 bedroom, 1.5 bath multi-level home in South Edgewood. This home offers large living room, dining room, large family room with fireplace, nice size kitchen, lots of storage, lower level garage with additional workshop area, and laundry room. The large sunroom on the rear of the...
1916 South Winding Way Anderson, IN
3 beds, 1 baths, 2,134 sqft, $124,900
Listing Agent: Kurstin Griffin
Courtesy of: RE/MAX Real Estate Solutions
30 Pictures
Walk in and Be Impressed! Updated & Renovated & Ready For You to Call Your New Home! Painted Thru-out! New Floor Coverings! Brand New Kitchen Cabinetry & Counter tops! New Gas Furnace! New Central Air! New Gas Water Heater! New white Masolite Doors & Hardware! New Trim! Updated Both...
932 Heritage Lane Anderson, IN
3 beds, 2 baths, 2,082 sqft, $125,000
Listing Agent: Gina Rininger
Courtesy of: F.C. Tucker Company
27 Pictures
Brick/Stone Ranch w/3 Bedrooms 1.5 Baths and Super Large 20 x 24 Family Room**Numerous Updates Include Roof in 2018, Furnace 3 Yrs. Old, Water Softener 1 Yr. Old & Added Insulation in the Attic 6 Months**Lots of Kitchen Cabinets & Counter tops, Center Island/Breakfast Bar &...
2714 West 39th Street Anderson, IN
3 beds, 1 baths, 1,858 sqft, $127,900
Listing Agent: Mary Lou Gordon
Courtesy of: RE/MAX At The Crossing
Courtesy of: M.S. Woods Real Estate, LLC
Office Phone: 317-578-3220
Listing Agent: Susan Broyles
Updated: 15th October, 2019 7:03 AM.
3407 Redwood Road Anderson, IN


text:
Searching Content indexed under Insurance Laws and Products by Richard Curd ordered by Published Date Descending.
Solicitors PI: SRA Consultation On Important Proposed Changes To Conflict Rules
The deadline for responses to the Solicitors Regulation Authorityís second consultation on changes to Rule 3 (Conflicts of Interest) and Rule 4 (Confidential Information) of the Solicitorsí Code of Conduct is 12 February 2010.
23 Feb 2010
Solicitors PI: The End For The Assigned Risks Pool?
In November 2009 the Solicitors Regulation Authority opened a consultation on the future of the Assigned Risks Pool which, the chair of the SRA has said, is "costing a huge amount of money but demonstrating little benefit".
8 Jan 2010
Insolvency Exposure For Professional Indemnity Insurers
In an important judgment for professional indemnity insurers, the Court has decided that whilst discharge from bankruptcy will mean that a client can no longer pursue a bankrupt professional in respect of his bankruptcy debts (including potential liability for professional negligence) it is possible for the client to pursue the bankruptís professional indemnity insurers direct under the Third Parties (Rights Against Insurers) Act 1930 using the proof of debt mechanism.
18 Dec 2007
Auditors and third party liability ñ judicial reassurance
The issue of auditorsí liability to third parties has been brought sharply into focus recently with The Company Law Reform Bill which embodies some significant changes in relation to auditorsí liability, namely the ability to negotiate "Liability Limitation Agreements".
16 Mar 2006
Causation in Professional Indemnity: is "but for" ever enough?
In an important judgment for professionals in all fields and their Insurers, the Court of Appeal yesterday applied Saamco principles to losses caused by the downturn in fortunes of Equitable Life and reached some very clear conclusions.
23 Feb 2006


text:
31 May 2019
Michael Springer
Department of Systems Biology
Harvard Medical School
Cells in nature are often faced with conflicting signals from which they have to make a single decision. To uncover design principles/motifs of cellular decision-making, my lab uses the galactose response in Saccharomyces cerevisiae as a model. Careful quantitative multidimensional dissection of the pathway has uncovered qualitative behaviors that had been missed in the many years of analysis of the pathway. These behaviors include ratiometric sensing, decoupling of the decision to induce and the level of induction, and flux sensing. I will discuss these design principles/motifs, the mechanisms that underlie them, and some physiological consequences of these designs.
current theory lunch schedule


text:
What would you like to know?
Mortgage Calculator
For new mortgages, if the downpayment or equity is less then 20% of the purchase price, the amortization cannot exceed 25 years and the maximum purchase price must be less than $1,000,000.
Mortgage rates are estimates of current rates. No fees are included.
What is your availability for a viewing?
303 1004 Wolfe Avenue, Vancouver
$480,000Calculate Mortgage Calculate Mortgage
780 Sq.Ft. 1 Baths 1 Beds 1973 Built
Google Maps Map WalkScore Email Us Request a Viewing
Virtual Tour
Character and History. This home has all the features of Vancouver's historical homes with character. Beautiful hardwood floors, coved doorways and open spaces. Situated in the corner and on the top floor, this home has light and views of the trees. Now is your chance to move into a well-cared for and loved 780' one bedroom and den in the heart of Vancouver. No car? no problem. This home has access close by to transit, shopping, parks and recreation. Pet Restrictions: No Dogs / Cats okay.
Taxes (2019): $1,247.70
Bike Room Garden Shared Laundry
Drapes Window Coverings Refrigerator Stove


text:
Egan Bernal says that staying relaxed and just trying to enjoy the racing helped him win this year's Tour de France. "All I could do was my best," he reasoned in an apparently successful effort to dissipate external pressures.
Bernal became the first Colombian winner of the Tour de France and Team Ineos's seventh winner in eight years after edging out team-mate and last year's winner, Geraint Thomas, by 1m11s.
"Maybe the key to winning the Tour is just trying to enjoy it," he said. "During the Tour, the media talk about pressure, pressure, pressure, but if I'd started to think about that, maybe I wouldn't have ridden the same. Maybe when I had a bad day or didn't have the best feeling, I would have started to think about the pressure.
"But throughout the Tour I knew I had prepared in the best way I could. What could I do then? Just enjoy it and ride as fast as I could. I couldn't control what the other guys would do or how they would ride, so all I could do was my best.
"I knew I would do my best so I would have been happy with any result – either winning the Tour or helping G win the Tour. In the end I just knew I needed to go full gas, enjoy the race, and stay calm."
While training for the Giro d'Italia earlier in the year, Bernal crashed and broke his collarbone.
"It wasn't difficult to stay motivated after missing the Giro," he said. "Mostly I was sad for my teammates, who had trained so hard to help me at the Giro. That was my first thought.
"But when I crashed I was in really good form and I said, 'okay.' I knew I didn't have much time to prepare for the Tour and I was starting with really good form. For the time I was off the bike I tried to stay focused with my routine. It worked."
Bernal was guided by coach Xabi Artetxe, who has looked after the 22-year-old since he joined what was then Team Sky at the start of last season.
"He is like my Dad in the team," said Bernal, "so when I'm doing things really well he will praise me, but when I'm not, he will say: 'Hey you are not doing really well, you need to do this and this.'"
He added: "At any moment if I had a doubt about my preparation or was not sure about winning the Tour, he was calm. For example when I was fifth he was saying to me: 'You are just 30 seconds from winning the Tour.'"
Nevertheless, Bernal said it was only after stage 19, when he took the yellow jersey, that he thought he could win, "because when you take the yellow it gives you some extra powers; it is more difficult to lose it."
Alex has written for more cricket publications than the rest of the road.cc team combined. Despite the apparent evidence of this picture, he doesn't especially like cake.


text:
- Venue
- The Phyllis Harriman Mason Gallery
- Location
- United States
The only real gallery that New York would permit you to visit within the course of six days in September is the Phyllis Harman Gallery in New York City at 215 West 57th Street. The teachers achieved a display of their work with assistance by the students and there was a lovely collection of subject matter deemed either too traditional or conventional with the rare startling medium-based piece.
The Arts Students League of New York is well respected for developing the talents of students with the wide choice of courses exploring painting, drawing, sculpture, moulding students into the next De Kooning, Warhol, O'Keefe etc. (Of course, these names are a general idea of the ability this League has, not having necessarily taught the likes of those mentioned).
There is an attractive collection of work that precedes the success of the gallery's previous exhibition each time, showcasing the depth of the teacher's knowledge of Fine Art with traditional and uncharacteristic subject matter. Among the different processes and areas explored, we were subjected to the likes of anatomy and life drawing; sculpture, printmaking – etching and lithography; watercolour; relief sculpture; collage, mixed media; photo and digital lithography; pastel from life; combined media assemblage; forging; portraiture and composition; mural painting; still life and landscape; modelling in clay; experimental techniques and many more in the hundreds.
There is a feeling of judging an art competition that overcomes the audience, viewing these pieces individually and deciding which is more suited our own styles and therefore which tutor is teaching the subject we would like to enrol in. Nevertheless, there were some very worthwhile artists we ought to keep our eyes open for whenever we hear of the League: Gregory Lyde Vigrass' Abstract Expressionist paintings which drastically control the motion and direction of the paintbrush, inspiring student Sasha Kogan to explore Abstract portrait painting; the art of James Garvey, which is forged, produces bent furniture designs which has inspired the likes of student K-thor Nielsen to create outdoor sculptures forged into Spanish designs. The wide-ranging printmaking techniques of Sylvie G. Covey shows a form of psychedelic etchings and digital lithographs we would not see again until we notice her student John Salvi's own take on Surrealist-esque prints; Naomi Campbell is a watercolour artist that proves having the same name as a famous model does not have her work suffer, with startling watercolours exploring human figure in history inspiring a similar exploration of shape with student Laura Egelhoff.
Many student exhibitions are instigated every year in every institute imaginable yet the rare numbers of teachers/instructors exhibitions shows a faith in the art education department not separating the old from the young but the artists from the artists. A nice presentation of art with traditional subject matter and non-traditional mediums demonstrating there is likely a class for you.


text:
Oracle Support to PeopleSoft HCM 8.9 and 9.0 Tech
Oracle proxy authentication allows a privileged user to connect to the Oracle instance using their own credentials but as a different user, thus automatically inheriting the user's privileges and permissions.... Oracle has also a nice mobile version of Oracle Support working on the mobi platform. You can use your mobile to update your pending SR's and quickly see your Open SR's and SR�
How to upload big large file to Oracle Support for Service
Creating a Service Request (SR) for an Oracle SN Issue The process for reporting Oracle SN issues to Oracle Support is the same process as used to report issues with any standard Oracle Product....2010-09-02�� Requesting a Service Request escalation via My Oracle Support is also an option; however, the same escalation information must be provided in the Service Request. Please be aware that Service Request updates may not be reviewed immediately and may contribute to delays in acknowledging and handling your escalation request. To avoid delays in handling and acknowledging �
Worry No More! Say Goodbye to Pain and Frustration when
2017-02-27�� 7) If recommended solution doesn�t work, you can always create a Service Request from this tool itself how to set up dell sas 6 � Create SR and TAR support with Oracle and resolved ORA-00600 errors successfully. � Inspected alert log file, monitored tablespaces and partitions that span across several tablespaces.. How to contact uber support canada
How To Create Sr In Oracle Support
How to Upload Files to Oracle Support AZIZ's BLOG
- Oracle on VMware vSphere & vSAN – Asks the Oracles
- My Oracle Support Mobile Oracle EBS and Applications Blog
- How to Create Oracle Service Request (SR) with Oracle
- How to Solve ORA-04031 with Oracle Support Provided Tool
How To Create Sr In Oracle Support
2014-10-29�� This video will teach you the concept of user role in oracle database which is an extension to the user privileges. In this video I did 10 SQL queries on user Roles which makes this tutorial fun.
- How to Create Oracle Service Request (SR) with Oracle {https://support.oracle.com} This tutorial will explain the steps on creating service request with oracle support First ensure you have valid credentials for logging to the oracle website Open any of your web browser like google, Firefox, Bing etc... Enter the link manually on your browser as shown below. OR . Click on the link Oracle
- 2017-06-19�� What the above means is, if in any case Oracle Support were to refuse assistance after opening an SR, no need to panic, simply call to open a ticket or open a ticket online with VMware GSS and make sure that the ticket is earmarked for �Oracle DBA team� within the GSS organization.
- Oracle has also a nice mobile version of Oracle Support working on the mobi platform. You can use your mobile to update your pending SR's and quickly see your Open SR's and SR�
- Oracle Auto Service Request�included with Oracle Premier Support�is the fastest route to restoring system availability if a hardware fault occurs. Take advantage of a secure, expedited diagnostic process with automatic service request generation, priority service request handling, and automatic parts dispatch�at no extra cost.
You can find us here:
- Australian Capital Territory: Waramanga ACT, Franklin ACT, Reid ACT, Fyshwick ACT, Dubbo ACT, ACT Australia 2653
- New South Wales: Eatonsville NSW, Caragabal NSW, Chakola NSW, Toms Creek NSW, Fairfield West NSW, NSW Australia 2033
- Northern Territory: Lyons NT, Karama NT, Mimili NT, Ciccone NT, Alawa NT, Bulman NT, NT Australia 0846
- Queensland: Howard QLD, Mt Crosby QLD, Dinmore QLD, The Glen QLD, QLD Australia 4097
- South Australia: The Pines SA, Halbury SA, Rosedale SA, Mount Eba SA, Lake Torrens Station SA, Keilira SA, SA Australia 5049
- Tasmania: Currie TAS, Sorell Creek TAS, Premaydena TAS, TAS Australia 7017
- Victoria: Moggs Creek VIC, Turtons Creek VIC, Gooram VIC, Baranduda VIC, Pigeon Ponds VIC, VIC Australia 3004
- Western Australia: Kurrawang community WA, Warwick WA, Marmion WA, WA Australia 6079
- British Columbia: West Kelowna BC, Parksville BC, Kamloops BC, Enderby BC, Creston BC, BC Canada, V8W 1W2
- Yukon: Yukon Crossing YT, Aishihik YT, Fort Selkirk YT, Carcross Cutoff YT, Upper Liard YT, YT Canada, Y1A 9C2
- Alberta: Cardston AB, Vulcan AB, St. Albert AB, Champion AB, Morrin AB, Thorsby AB, AB Canada, T5K 5J6
- Northwest Territories: Jean Marie River NT, Salt Plains 195 NT, Norman Wells NT, Nahanni Butte NT, NT Canada, X1A 5L6
- Saskatchewan: Dysart SK, Vonda SK, Swift Current SK, Cadillac SK, Imperial SK, Macrorie SK, SK Canada, S4P 5C2
- Manitoba: Gilbert Plains MB, Manitou MB, Elkhorn MB, MB Canada, R3B 9P4
- Quebec: Lac-Saguay QC, Tadoussac QC, Saint-Basile QC, Bromont QC, Pointe-Lebel QC, QC Canada, H2Y 5W4
- New Brunswick: Lameque NB, Atholville NB, St. Martins NB, NB Canada, E3B 5H9
- Nova Scotia: North Sydney NS, Pictou NS, Glace Bay NS, NS Canada, B3J 2S6
- Prince Edward Island: Souris West PE, Darlington PE, Georgetown PE, PE Canada, C1A 9N6
- Newfoundland and Labrador: Little Bay NL, York Harbour NL, St. Bernard's-Jacques Fontaine NL, St. Anthony NL, NL Canada, A1B 1J2
- Ontario: Greater Sudbury ON, Dalhousie Lake ON, Killaloe ON, Marlbank, Yearley ON, Temagami ON, Fanshawe Lake ON, ON Canada, M7A 5L7
- Nunavut: Eskimo Point (Arviat) NU, Arviat NU, NU Canada, X0A 6H7
- England: Salford ENG, Colchester ENG, Sunderland ENG, Preston ENG, Runcorn ENG, ENG United Kingdom W1U 1A5
- Northern Ireland: Craigavon�(incl. Lurgan, Portadown) NIR, Bangor NIR, Derry�(Londonderry) NIR, Newtownabbey NIR, Craigavon�(incl. Lurgan, Portadown) NIR, NIR United Kingdom BT2 4H3
- Scotland: Livingston SCO, Dundee SCO, Kirkcaldy SCO, Glasgow SCO, Paisley SCO, SCO United Kingdom EH10 9B8
- Wales: Wrexham WAL, Barry WAL, Cardiff WAL, Swansea WAL, Wrexham WAL, WAL United Kingdom CF24 9D5


text:
Looking for design inspiration? Browse our curated collections!
Boundary: Bleed area may not be visible.
Click and drag to re-position the image, if desired.
The watermark at the lower right corner of the image will not appear on the final product.
Image Size
The image is near the edges of the product but doesn't cover the entire product. Some of the background color may appear around the outside edges of the image.
Background Color
Queen duvet cover (88" x 88") featuring the image "Early Spring IIi Daffodil Series" by Shadia Derbyshire. Our soft microfiber duvet covers are hand sewn and include a hidden zipper for easy washing and assembly. Your selected image is printed on the top surface with a soft white surface underneath. All duvet covers are machine washable with cold water and a mild detergent.
Ships Within
2 - 3 business days
View All & Comment
Canvas Print
Framed Print
Metal Print
Acrylic Print
Wood Print
Greeting Card
Phone Case
Throw Pillow
Duvet Cover
Shower Curtain
Tote Bag
Round Beach Towel
Carry-All Pouch
Beach Towel
Weekender Tote Bag
Portable Battery Charger
Bath Towel
Yoga Mats
Spiral Notebooks
Fleece Blankets
Congratulation on your recent sale!!
Congratulations on your sale Shadia!
Average Rating (4.82 Stars):
Mizz Tavi
August 26th, 2019
Lesley McDonald
August 25th, 2019
Early Spring - Daffodil Series - Mixed Media Collage & Acrylic on Canvas These elegant daffodils were created by Metro Detroit fine artist Shadia. She began with a background surface in shimmering metallic hues of deep magenta and vibrant green. Using acrylic, colorsafe spray paints, she added texture and pattern. Next, the artist created a delicate underpainting of each daffodil. Using a selection of hand painted found collage papers, tissues, discarded sewing patterns and scrapbook papers, Shadia painstakingly collaged each blossom as well as a portion of the background. The result is a simple composed yet dynamic painting in complementary hues of reddish-purples and and yellow, with subtle texture and pattern, and a glimmering surface of metallic paints. Each daffodil measures 9"x9" and is painted on luxuriously deep 2.25" museum canvas. The surface is coated with a gloss UV top coat to preserve the colors and protect against fading. The edges of this panel are painted black and...
My most recent work draws more inspiration from color and texture than distinct subject matters, and my objective remains to imbue my canvasses with a sense of emotion. Through the intricate texture and vibrant color in my pieces, I aim to create a visceral response in the viewer; my hope is that the viewer may connect with them emotionally rather than through conscious, deliberate effort. In this way, viewers create their own interpretations through this initial connection, and the narrative of the piece becomes secondary. I was born and raised in Southeastern Michigan. A few years after earning my BFA with Honors from Eastern Michigan University, I began a mural and decorative painting company which allows me to create beautiful...
Our return policy is very simple:
If you're not happy with a purchase that you made on FineArtAmerica.com, for any reason, you can return it to us within 30 days of the order date. As soon as it arrives, we'll issue a full refund for the entire purchase price. Please note - Fine Art America does not reimburse the outgoing or return shipping charges unless the return is due to a defect in quality.
Fine Art America sells thousands of pieces of artwork each month - all with a 100% money-back guarantee. We take great pride in the fact that hundreds of thousands of artists have chosen Fine Art America to fulfill their orders, and we look forward to helping you select your next piece!
All duvet covers ship from our production facility within 2 - 3 business days of your order.


text:
Ricin toxin (RT) ranks at the top of the list of bioweapons of concern to civilian and military personnel alike, due to its high potential for morbidity and mortality after inhalation. In nonhuman primates, aerosolized ricin triggers severe acute respiratory distress characterized by perivascular and alveolar edema, neutrophilic infiltration, and severe necrotizing bronchiolitis and alveolitis. There are currently no approved countermeasures for ricin intoxication. Here, we report the therapeutic potential of a humanized mAb against an immunodominant epitope on ricin's enzymatic A chain (RTA). Rhesus macaques that received i.v. huPB10 4 hours after a lethal dose of ricin aerosol exposure survived toxin challenge, whereas control animals succumbed to ricin intoxication within 30 hours. Antibody intervention at 12 hours resulted in the survival of 1 of 5 monkeys. Changes in proinflammatory cytokine, chemokine, and growth factor profiles in bronchial alveolar lavage fluids before and after toxin challenge successfully clustered animals by treatment group and survival, indicating a relationship between local tissue damage and experimental outcome. This study represents the first demonstration, to our knowledge, in nonhuman primates that the lethal effects of inhalational ricin exposure can be negated by a drug candidate, and it opens up a path forward for product development.
Recent and ongoing outbreaks of Ebola virus disease (EVD) underscore the unpredictable nature of ebolavirus reemergence and the urgent need for antiviral treatments. Unfortunately, available experimental vaccines and immunotherapeutics are specific for a single member of the Ebolavirus genus, Ebola virus (EBOV), and ineffective against other ebolaviruses associated with EVD, including Sudan virus (SUDV) and Bundibugyo virus (BDBV). Here we show that MBP134AF, a pan-ebolavirus therapeutic comprising two broadly neutralizing human antibodies (bNAbs), affords unprecedented effectiveness and potency as a therapeutic countermeasure to antigenically diverse ebolaviruses. MBP134AF could fully protect ferrets against lethal EBOV, SUDV, and BDBV infection, and a single 25-mg/kg dose was sufficient to protect NHPs against all three viruses. The development of MBP134AF provides a successful model for the rapid discovery and translational advancement of immunotherapeutics targeting emerging infectious diseases.
AssuntosAnticorpos Monoclonais/imunologia , Anticorpos Monoclonais/uso terapêutico , Anticorpos Antivirais/imunologia , Anticorpos Antivirais/uso terapêutico , Ebolavirus/patogenicidade , Furões/virologia , Doença pelo Vírus Ebola/imunologia , Doença pelo Vírus Ebola/prevenção & controle , Bem-Estar do Animal , Animais , Anticorpos Monoclonais/administração & dosagem , Anticorpos Monoclonais/isolamento & purificação , Anticorpos Neutralizantes/administração & dosagem , Anticorpos Neutralizantes/imunologia , Anticorpos Neutralizantes/isolamento & purificação , Anticorpos Neutralizantes/uso terapêutico , Anticorpos Antivirais/administração & dosagem , Linhagem Celular , Cercopithecus aethiops , Modelos Animais de Doenças , Feminino , Filoviridae/imunologia , Infecções por Filoviridae/imunologia , Infecções por Filoviridae/prevenção & controle , Infecções por Filoviridae/virologia , Glicoproteínas/imunologia , Cobaias , Células HEK293 , Doença pelo Vírus Ebola/virologia , Humanos , Células Matadoras Naturais , Macaca , Macaca fascicularis , Masculino , Primatas , Análise de Sobrevida , Resultado do Tratamento , Proteínas Virais/imunologia
Passive administration of monoclonal antibodies (mAbs) is a promising therapeutic approach for Ebola virus disease (EVD). However, all mAbs and mAb cocktails that have entered clinical development are specific for a single member of the Ebolavirus genus, Ebola virus (EBOV), and ineffective against outbreak-causing Bundibugyo virus (BDBV) and Sudan virus (SUDV). Here, we advance MBP134, a cocktail of two broadly neutralizing human mAbs, ADI-15878 from an EVD survivor and ADI-23774 from the same survivor but specificity-matured for SUDV GP binding affinity, as a candidate pan-ebolavirus therapeutic. MBP134 potently neutralized all ebolaviruses and demonstrated greater protective efficacy than ADI-15878 alone in EBOV-challenged guinea pigs. A second-generation cocktail, MBP134AF, engineered to effectively harness natural killer (NK) cells afforded additional improvement relative to its precursor in protective efficacy against EBOV and SUDV in guinea pigs. MBP134AF is an optimized mAb cocktail suitable for evaluation as a pan-ebolavirus therapeutic in nonhuman primates.
AssuntosAnticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Ebolavirus/imunologia , Doença pelo Vírus Ebola/imunologia , Doença pelo Vírus Ebola/prevenção & controle , Bem-Estar do Animal , Animais , Anticorpos Monoclonais/administração & dosagem , Anticorpos Monoclonais/isolamento & purificação , Anticorpos Monoclonais/uso terapêutico , Anticorpos Neutralizantes/administração & dosagem , Anticorpos Neutralizantes/imunologia , Anticorpos Neutralizantes/isolamento & purificação , Anticorpos Neutralizantes/uso terapêutico , Anticorpos Antivirais/administração & dosagem , Anticorpos Antivirais/uso terapêutico , Antivirais , Modelos Animais de Doenças , Ebolavirus/patogenicidade , Epitopos/imunologia , Feminino , Filoviridae/imunologia , Cobaias , Doença pelo Vírus Ebola/virologia , Humanos , Imunoterapia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Knockout , Proteínas Recombinantes/imunologia , Resultado do Tratamento
As observed during the 2013-2016 Ebola virus disease epidemic, containment of filovirus outbreaks is challenging and made more difficult by the lack of approved vaccine or therapeutic options. Marburg and Ravn viruses are highly virulent and cause severe and frequently lethal disease in humans. Monoclonal antibodies (mAbs) are a platform technology in wide use for autoimmune and oncology indications. Previously, we described human mAbs that can protect mice from lethal challenge with Marburg virus. We demonstrate that one of these mAbs, MR191-N, can confer a survival benefit of up to 100% to Marburg or Ravn virus-infected rhesus macaques when treatment is initiated up to 5 days post-inoculation. These findings extend the small but growing body of evidence that mAbs can impart therapeutic benefit during advanced stages of disease with highly virulent viruses and could be useful in epidemic settings.
AssuntosAnticorpos Monoclonais/uso terapêutico , Infecções por Filoviridae/tratamento farmacológico , Filoviridae/fisiologia , Doença do Vírus de Marburg/tratamento farmacológico , Marburgvirus/fisiologia , Animais , Proteção Cruzada , Infecções por Filoviridae/virologia , Cobaias , Humanos , Macaca fascicularis , Macaca mulatta , Doença do Vírus de Marburg/virologia , Projetos Piloto
PB10 is a murine monoclonal antibody against an immunodominant epitope on ricin toxin's enzymatic subunit. Here, we characterize a fully humanized version of PB10 IgG1 (hPB10) and demonstrate that it has potent in vitro and in vivo toxin-neutralizing activities. We also report the minimum serum concentrations of hPB10 required to protect mice against 10 times the 50% lethal dose of ricin when delivered by injection and inhalation.
AssuntosAnticorpos Monoclonais Humanizados/administração & dosagem , Anticorpos Monoclonais/administração & dosagem , Antitoxinas/administração & dosagem , Substâncias para a Guerra Química/toxicidade , Envenenamento/terapia , Ricina/toxicidade , Animais , Anticorpos Monoclonais/isolamento & purificação , Anticorpos Monoclonais/farmacologia , Anticorpos Monoclonais Humanizados/isolamento & purificação , Anticorpos Monoclonais Humanizados/farmacologia , Antitoxinas/isolamento & purificação , Antitoxinas/farmacologia , Modelos Animais de Doenças , Feminino , Camundongos Endogâmicos BALB C , Análise de Sobrevida , Resultado do Tratamento
Countermeasures against potential biothreat agents remain important to US Homeland Security, and many of these pharmaceuticals could have dual use in the improvement of global public health. Junin virus, the causative agent of Argentine hemorrhagic fever (AHF), is an arenavirus identified as a category A high-priority agent. There are no Food and Drug Administration (FDA) approved drugs available for preventing or treating AHF, and the current treatment option is limited to administration of immune plasma. Whereas immune plasma demonstrates the feasibility of passive immunotherapy, it is limited in quantity, variable in quality, and poses safety risks such as transmission of transfusion-borne diseases. In an effort to develop a monoclonal antibody (mAb)-based alternative to plasma, three previously described neutralizing murine mAbs were expressed as mouse-human chimeric antibodies and evaluated in the guinea pig model of AHF. These mAbs provided 100% protection against lethal challenge when administered 2 d after infection (dpi), and one of them (J199) was capable of providing 100% protection when treatment was initiated 6 dpi and 92% protection when initiated 7 dpi. The efficacy of J199 is superior to that previously described for all other evaluated drugs, and its high potency suggests that mAbs like J199 offer an economical alternative to immune plasma and an effective dual use (bioterrorism/public health) therapeutic.
AssuntosAnticorpos Monoclonais/farmacologia , Anticorpos Antivirais/farmacologia , Febre Hemorrágica Americana/tratamento farmacológico , Febre Hemorrágica Americana/imunologia , Animais , Anticorpos Monoclonais/genética , Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/genética , Anticorpos Antivirais/imunologia , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Cobaias , Humanos , Vírus Junin , Camundongos , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Proteínas Recombinantes de Fusão/farmacologia
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) was identified in 2012 as the causative agent of a severe, lethal respiratory disease occurring across several countries in the Middle East. To date there have been over 1600 laboratory confirmed cases of MERS-CoV in 26 countries with a case fatality rate of 36%. Given the endemic region, it is possible that MERS-CoV could spread during the annual Hajj pilgrimage, necessitating countermeasure development. In this report, we describe the clinical and radiographic changes of rhesus monkeys following infection with 5×10(6) PFU MERS-CoV Jordan-n3/2012. Two groups of NHPs were treated with either a human anti-MERS monoclonal antibody 3B11-N or E410-N, an anti-HIV antibody. MERS-CoV Jordan-n3/2012 infection resulted in quantifiable changes by computed tomography, but limited other clinical signs of disease. 3B11-N treated subjects developed significantly reduced lung pathology when compared to infected, untreated subjects, indicating that this antibody may be a suitable MERS-CoV treatment.
AssuntosAnticorpos Monoclonais/administração & dosagem , Anticorpos Antivirais/administração & dosagem , Infecções por Coronavirus/tratamento farmacológico , Infecções por Coronavirus/patologia , Pulmão/patologia , Coronavírus da Síndrome Respiratória do Oriente Médio/fisiologia , Animais , Infecções por Coronavirus/virologia , Modelos Animais de Doenças , Humanos , Pulmão/virologia , Macaca mulatta , Masculino
We have produced and characterized two chimeric human IgG1 monoclonal antibodies that bind different immunodominant epitopes on Vibrio cholerae lipopolysaccharide (LPS). MAb 2D6 IgG1 recognizes Ogawa O-polysaccharide antigen, while mAb ZAC-3 IgG1 recognizes core/lipid A moiety of Ogawa and Inaba LPS. Both antibodies were expressed using a Nicotiana benthamiana-based rapid antibody-manufacturing platform (RAMP) and evaluated in vitro for activities associated with immunity to V. cholerae, including vibriocidal activity, bacterial agglutination and motility arrest.
AssuntosAnticorpos Antibacterianos/imunologia , Anticorpos Monoclonais/imunologia , Epitopos Imunodominantes/imunologia , Imunoglobulina G/imunologia , Vibrio cholerae/imunologia , Anticorpos Antibacterianos/biossíntese , Anticorpos Antibacterianos/genética , Anticorpos Monoclonais/biossíntese , Anticorpos Monoclonais/genética , Antígenos de Bactérias/imunologia , Quimera/imunologia , Clonagem Molecular , Epitopos/imunologia , Humanos , Imunoglobulina G/biossíntese , Imunoglobulina G/genética , Lipopolissacarídeos/imunologia , Antígenos O/imunologia , Tabaco/genética
Due to the fast-acting nature of ricin, staphylococcal enterotoxin B (SEB), and Clostridium perfringens epsilon toxin (ETX), it is necessary that therapeutic interventions following a bioterrorism incident by one of these toxins occur as soon as possible after intoxication. Moreover, because the clinical manifestations of intoxication by these agents are likely to be indistinguishable from each other, especially following aerosol exposure, we have developed a cocktail of chimeric monoclonal antibodies that is capable of neutralizing all three toxins. The efficacy of this cocktail was demonstrated in mouse models of lethal dose toxin challenge.
AssuntosAnticorpos Monoclonais/farmacologia , Toxinas Bacterianas/toxicidade , Bioterrorismo/prevenção & controle , Enterotoxinas/toxicidade , Envenenamento/prevenção & controle , Proteínas Recombinantes de Fusão/farmacologia , Ricina/toxicidade , Animais , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Testes de Neutralização
Without an approved vaccine or treatments, Ebola outbreak management has been limited to palliative care and barrier methods to prevent transmission. These approaches, however, have yet to end the 2014 outbreak of Ebola after its prolonged presence in West Africa. Here we show that a combination of monoclonal antibodies (ZMapp), optimized from two previous antibody cocktails, is able to rescue 100% of rhesus macaques when treatment is initiated up to 5 days post-challenge. High fever, viraemia and abnormalities in blood count and blood chemistry were evident in many animals before ZMapp intervention. Advanced disease, as indicated by elevated liver enzymes, mucosal haemorrhages and generalized petechia could be reversed, leading to full recovery. ELISA and neutralizing antibody assays indicate that ZMapp is cross-reactive with the Guinean variant of Ebola. ZMapp exceeds the efficacy of any other therapeutics described so far, and results warrant further development of this cocktail for clinical use.
AssuntosAnticorpos Monoclonais/uso terapêutico , Anticorpos Antivirais/uso terapêutico , Doença pelo Vírus Ebola/tratamento farmacológico , Imunização Passiva , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Neutralizantes/imunologia , Anticorpos Neutralizantes/uso terapêutico , Anticorpos Antivirais/imunologia , Reações Cruzadas/imunologia , Ebolavirus/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Guiné , Cobaias , Doença pelo Vírus Ebola/sangue , Doença pelo Vírus Ebola/imunologia , Doença pelo Vírus Ebola/virologia , Macaca mulatta/imunologia , Macaca mulatta/virologia , Masculino , Dados de Sequência Molecular , Alinhamento de Sequência , Proteínas do Envelope Viral/química , Proteínas do Envelope Viral/imunologia , Viremia/tratamento farmacológico , Viremia/imunologia , Viremia/virologia
Respiratory syncytial virus (RSV) can cause devastating lower respiratory tract infections in preterm infants or when other serious health problems are present. Immunoprophylaxis with palivizumab (Synagis), a humanized IgG1 mAb, is the current standard of care for preventing RSV infection in at-risk neonates. We have explored the contribution of effector function to palivizumab efficacy using a plant-based expression system to produce palivizumab N-glycan structure variants with high homogeneity on different antibody isotypes. We compared these isotype and N-glycoform variants with commercially available palivizumab with respect to both in vitro receptor and C1q binding and in vivo efficacy. Whereas the affinity for antigen and neutralization activity of each variant were indistinguishable from those of palivizumab, their Fcγ receptor binding profiles were very different, which was reflected in either a reduced or enhanced ability to influence the RSV lung titer in challenged cotton rats. Enhanced Fcγ receptor binding was associated with reduced viral lung titers compared with palivizumab, whereas abrogation of receptor binding led to a drastic reduction in efficacy. The results support the hypotheses that classic antibody neutralization is a minor component of efficacy by palivizumab in the cotton rat and that antibody-dependent cell-mediated cytotoxicity activity can significantly enhance the efficacy of this antiviral mAb.
AssuntosAnticorpos Antivirais/química , Anticorpos Antivirais/uso terapêutico , Polissacarídeos/metabolismo , Infecções por Vírus Respiratório Sincicial/tratamento farmacológico , Infecções por Vírus Respiratório Sincicial/imunologia , Vírus Sinciciais Respiratórios/imunologia , Sigmodontinae/virologia , Animais , Anticorpos Monoclonais/uso terapêutico , Anticorpos Neutralizantes/farmacologia , Complemento C1q/metabolismo , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Pulmão/efeitos dos fármacos , Pulmão/imunologia , Pulmão/patologia , Pulmão/virologia , Masculino , Testes de Neutralização , Ligação Proteica/efeitos dos fármacos , Receptores Fc/metabolismo , Vírus Sinciciais Respiratórios/efeitos dos fármacos , Sigmodontinae/imunologia , Resultado do Tratamento
Recent incidents in the United States and abroad have heightened concerns about the use of ricin toxin as a bioterrorism agent. In this study, we produced, using a robust plant-based platform, four chimeric toxin-neutralizing monoclonal antibodies that were then evaluated for the ability to passively protect mice from a lethal-dose ricin challenge. The most effective antibody, c-PB10, was further evaluated in mice as a therapeutic following ricin exposure by injection and inhalation.
AssuntosAntitoxinas/administração & dosagem , Imunização Passiva/métodos , Planticorpos/administração & dosagem , Envenenamento/prevenção & controle , Ricina/antagonistas & inibidores , Ricina/toxicidade , Animais , Anticorpos Monoclonais/administração & dosagem , Anticorpos Neutralizantes/administração & dosagem , Feminino , Camundongos Endogâmicos BALB C , Análise de Sobrevida , Resultado do Tratamento
Severe lower respiratory tract infection in infants and small children is commonly caused by respiratory syncytial virus (RSV). Palivizumab (Synagis(®)), a humanized IgG1 monoclonal antibody (mAb) approved for RSV immunoprophylaxis in at-risk neonates, is highly effective, but pharmacoeconomic analyses suggest its use may not be cost-effective. Previously described potent RSV neutralizers (human Fab R19 and F2-5; human IgG RF-1 and RF-2) were produced in IgG format in a rapid and inexpensive Nicotiana-based manufacturing system for comparison with palivizumab. Both plant-derived (palivizumab-N) and commercial palivizumab, which is produced in a mouse myeloma cell line, showed protection in prophylactic (p < 0.001 for both mAbs) and therapeutic protocols (p < 0.001 and p < 0.05 respectively). The additional plant-derived human mAbs directed against alternative epitopes displayed neutralizing activity, but conferred less protection in vivo than palivizumab-N or palivizumab. Palivizumab remains one of the most efficacious RSV mAbs described to date. Production in plants may reduce manufacturing costs and improve the pharmacoeconomics of RSV immunoprophylaxis and therapy.
AssuntosAnticorpos Monoclonais Humanizados/uso terapêutico , Anticorpos Neutralizantes/uso terapêutico , Vírus Sincicial Respiratório Humano/imunologia , Tabaco/imunologia , Animais , Anticorpos Monoclonais Humanizados/economia , Anticorpos Monoclonais Humanizados/imunologia , Anticorpos Neutralizantes/economia , Anticorpos Neutralizantes/imunologia , Modelos Animais de Doenças , Humanos , Palivizumab , Infecções por Vírus Respiratório Sincicial/imunologia , Infecções por Vírus Respiratório Sincicial/prevenção & controle , Infecções por Vírus Respiratório Sincicial/virologia , Sigmodontinae , Resultado do Tratamento
No countermeasures currently exist for the prevention or treatment of the severe sequelae of Filovirus (such as Ebola virus; EBOV) infection. To overcome this limitation in our biodefense preparedness, we have designed monoclonal antibodies (mAbs) which could be used in humans as immunoprotectants for EBOV, starting with a murine mAb (13F6) that recognizes the heavily glycosylated mucin-like domain of the virion-attached glycoprotein (GP). Point mutations were introduced into the variable region of the murine mAb to remove predicted human T-cell epitopes, and the variable regions joined to human constant regions to generate a mAb (h-13F6) appropriate for development for human use. We have evaluated the efficacy of three variants of h-13F6 carrying different glycosylation patterns in a lethal mouse EBOV challenge model. The pattern of glycosylation of the various mAbs was found to correlate to level of protection, with aglycosylated h-13F6 providing the least potent efficacy (ED(50) = 33 µg). A version with typical heterogenous mammalian glycoforms (ED(50) = 11 µg) had similar potency to the original murine mAb. However, h-13F6 carrying complex N-glycosylation lacking core fucose exhibited superior potency (ED(50) = 3 µg). Binding studies using Fcγ receptors revealed enhanced binding of nonfucosylated h-13F6 to mouse and human FcγRIII. Together the results indicate the presence of Fc N-glycans enhances the protective efficacy of h-13F6, and that mAbs manufactured with uniform glycosylation and a higher potency glycoform offer promise as biodefense therapeutics.
AssuntosEbolavirus/metabolismo , Fucose/imunologia , Animais , Anticorpos Monoclonais/química , Antivirais/química , Complemento C1q/química , Ebolavirus/imunologia , Feminino , Fucose/química , Glicosilação , Doença pelo Vírus Ebola/imunologia , Doença pelo Vírus Ebola/prevenção & controle , Humanos , Sistema Imunitário , Imunização Passiva , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Ressonância de Plasmônio de Superfície , Tabaco
BACKGROUND: Fc-glycosylation of monoclonal antibodies (mAbs) has profound implications on the Fc-mediated effector functions. Alteration of this glycosylation may affect the efficiency of an antibody. However, difficulties in the production of mAbs with homogeneous N-glycosylation profiles in sufficient amounts hamper investigations of the potential biological impact of different glycan residues. METHODOLOGY/PRINCIPAL FINDINGS: Here we set out to evaluate a transient plant viral based production system for the rapid generation of different glycoforms of a monoclonal antibody. Ebola virus mAb h-13F6 was generated using magnICON expression system in Nicotiana benthamiana, a plant species developed for commercial scale production of therapeutic proteins. h-13F6 was co-expressed with a series of modified mammalian enzymes involved in the processing of complex N-glycans. Using wild type (WT) plants and the glycosylation mutant ΔXTFT that synthesizes human like biantennary N-glycans with terminal N-acetylglucosamine on each branch (GnGn structures) as expression hosts we demonstrate the generation of h-13F6 complex N-glycans with (i) bisected structures, (ii) core α1,6 fucosylation and (iii) ß1,4 galactosylated oligosaccharides. In addition we emphasize the significance of precise sub Golgi localization of enzymes for engineering of IgG Fc-glycosylation. CONCLUSION: The method described here allows the efficient generation of a series of different human-like glycoforms at large homogeneity of virtually any antibody within one week after cDNA delivery to plants. This accelerates follow up functional studies and thus may contribute to study the biological role of N-glycan residues on Fcs and maximizing the clinical efficacy of therapeutic antibodies.
AssuntosAnticorpos Monoclonais/biossíntese , Ebolavirus/imunologia , Anticorpos Monoclonais/imunologia , Cromatografia Líquida , Eletroforese em Gel de Poliacrilamida , Glicosilação , Planticorpos/imunologia , Espectrometria de Massas por Ionização por Electrospray , Tabaco/imunologia
Plants have been proposed as an attractive alternative for pharmaceutical protein production to current mammalian or microbial cell-based systems. Eukaryotic protein processing coupled with reduced production costs and low risk for mammalian pathogen contamination and other impurities have led many to predict that agricultural systems may offer the next wave for pharmaceutical product production. However, for this to become a reality, the quality of products produced at a relevant scale must equal or exceed the predetermined release criteria of identity, purity, potency and safety as required by pharmaceutical regulatory agencies. In this article, the ability of transient plant virus expression systems to produce a wide range of products at high purity and activity is reviewed. The production of different recombinant proteins is described along with comparisons with established standards, including high purity, specific activity and promising preclinical outcomes. Adaptation of transient plant virus systems to large-scale manufacturing formats required development of virus particle and Agrobacterium inoculation methods. One transient plant system case study illustrates the properties of greenhouse and field-produced recombinant aprotinin compared with an US Food and Drug Administration-approved pharmaceutical product and found them to be highly comparable in all properties evaluated. A second transient plant system case study demonstrates a fully functional monoclonal antibody conforming to release specifications. In conclusion, the production capacity of large quantities of recombinant protein offered by transient plant expression systems, coupled with robust downstream purification approaches, offers a promising solution to recombinant protein production that compares favourably to cell-based systems in scale, cost and quality.
AssuntosAnticorpos Monoclonais/biossíntese , Aprotinina/biossíntese , Engenharia Genética/métodos , Plantas Geneticamente Modificadas/metabolismo , Proteínas Recombinantes/biossíntese , Anticorpos Monoclonais/imunologia , Aprotinina/imunologia , Vírus de Plantas , Plantas Geneticamente Modificadas/imunologia , Proteínas Recombinantes/imunologia , Rhizobium
Binding and competition of five Bacillus thuringiensis toxins--Cry1Ab, Cry1Ac, Cry1Ba, Cry1Ca, and Cry1Ea--for midgut binding sites from three pests, Spodoptera frugiperda, Diatraea saccharalis, and Diatraea grandiosella, were investigated as part of a strategy to develop tropical transgenic maize resistant to several stemborers. On S. frugiperda, Cry1Ab and Cry1Ac compete for the same binding site; Cry1Ba and Cry1Ca compete for a second binding site. Cry1Ea recognizes a third specific binding site in S. frugiperda and does not compete with any of the other toxins. On D. grandiosella and D. saccharalis, Cry1Ac competes with Cry1Ab and not with Cry1Ba and Cry1Ca. Cry1Ba and Cry1Ca recognize each a specific binding site and do not compete with any of the other four toxins. Cry1Ea does not recognize any binding site on Diatraea species. Combinations of toxins are proposed to develop transgenic maize resistant to the three stemborers while allowing resistance management.


text:
Feeling blue and desperately needed some rainbows. Hoping the day gets better; hoping to accomplish something; hoping to move forward as my focus shifts. Until then, settling for a sunset…Read More
All articles filed in Sunset
Mountain View
Sunset from above….Read More
Hazy Lazy Daze
When the haze lingers and a daze follows, the lazy hours pass by as i continue to type type type… Perhaps someday, rain will finally patter down and something new will follow. Coming soon, Never Forgetting You… Clover BooksRead More
Coming Undone
Beginning to think writing books is the cruelest thing ever. Just when you believe your story has meaning, flows well, and touches a soul, you realize those nasty pixies have been tinkering with your computer again. And the screws in your head have come loose once again, leaving you screaming in madness. That's when you've…Read More
Northwest Getaway
When you're still living out of a suitcase and have nowhere to be. Find a beach… Ocean ShoresRead More