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Position Summary
Company Overview:
Comprehensive Cancer Centers of Nevada is seeking a Registered Nurse to join our Research Team. CCCN develops and conducts more than 170 Phase I, Phase II and Phase III clinical research trials each year including genitourinary and gastrointestinal cancer treatments, radiation clinical trials, breast cancer and skin cancer. Motivated, caring professional are encouraged to join us.
Career Opportunity:
Comprehensive Cancer Centers of Nevada has an exciting opportunity for a Registered Nurse at our Twain clinic in Las Vegas, Nevada.
The essential functions for the Clinical Research Nurse - Oncology include the following:
Screens all potential and physician-referred patients for protocol eligibility. Maintains and submits monthly screening logs.
Presents trial concepts and details to the patients, and participates in the informed consent process under the direction of the physician.
Documents informed consent process and all components of protocol treatment. Participates in ongoing patient education. Coordinates scheduling of tests and treatments required for the protocol. Maintains research patient database according to US Oncology Research (USOR) Standard Operating Procedure (SOP).
Assesses patients for changes in condition, adverse events, concomitant medication use, protocol compliance, response to study drug, and thoroughly documents all findings.
Works directly with the physician/investigator and clinic staff to ensure protocol compliance.
Reports to practice operations and physicians regarding monthly accrual statistics and financial status. May be responsible for creating budgets for the research department. Coordinates payment of non-standard of care protocol-required procedures in order to be compliant with federal and state billing regulations.
Ensures accurate and complete data collection, entry, and timely submission to central operations in accordance with USOR SOP.
Coordinates research drug procurement and accountability records in accordance with USOR SOP.
Participates in and supports all site auditing and monitoring visits.
Maintains protocol regulatory documents in compliance with USOR SOP. Communicates, reviews, and documents weekly Regulatory Memoranda with physicians and other clinic staff as appropriate.
Oversees local quality assurance program and develops appropriate action plans for quality improvement.
Actively participates in protocol initiations and study specific educational programs. Communicates and collaborates on an ongoing basis with central operations.
Provides a safe environment for patients, families, and clinical staff at all time through compliance with all federal, state, and professional regulatory standards as issued through OSHA and the CDC. Maintains strict patient confidentiality according to HIPAA regulations and applicable law.
The ideal candidate will have the following background and experience:
Graduate from an accredited RN program and bachelors in science or healthcare field is required (BSN preferred).
Three years of clinical research experience preferred
Current licensure as a registered nurse in state of Nevada
Current BLCS or ACLS certification required.
SoCRA or ACRP certification preferred
Knowledge of medical terminology, nursing assessment, clinical trials and GCP concepts
Experience with computer data entry and database management
Ability to read, analyze and interpret technical procedures such as protocols, informed consent documents and regulatory document
Successful candidates will thrive in a fast paced, rapidly changing environment and have a passion for working with and providing assistance to patients.
Ready For Your Next Career Challenge? We'd Love to Hear from You!
If you possess the above qualifications and a desire to make a difference, we invite you to submit your resume and apply. In addition to a great career opportunity, we offer excellent benefits, a team environment, professional development, and the chance to be part of a nationwide network dedicated to fight the war against cancer. To apply please click on the Apply Now link.
Still have more questions? Please call our Nursing Hotline at 702-435-2977 to speak to an Oncology Nursing Leader who can give you more information on starting or continuing your career in oncology nursing.
Category: Clinical
The US Oncology Network is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability or protected veteran status.
Unitedhealth Group Inc.
Posted 4 days agoVIEW JOBS
US Oncology, Inc.


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About Our Global Programs
Our Global Programs & Global Ambassador Adventures get to the heart of marine conservation issues, driving positive change for the ocean and the communities who rely upon it for survival. Our Global Program in Kovalam and Vizhinjam, Kerala - southern India, was launched in February 2017 with 6 Global Ambassadors and 2 PCFML staff facilitating an intensive two-week programs to determine a long-term Masterplan for the region, alongside the local community.
Our campaign was an enormous success and led to:
The release of our 5-year Masterplan for the region, focused on fisheries management, waste management and waterway remediation, underpinned by educational programs to drive long-term positive change;
The opening of our Marine Conservation Centre on Eve's Beach, Kovalam in April, 2018;
The employment of our full-time team in the region to run our centre and roll-out our initiatives from May, 2018;
The implementation of the first local community and household multi-stream waste collection service in April 2019;
The development of our trial CAP systems to produce aquaponic and composting systems from collected plastics in order to provide a solution to local waste, which takes pressure off wild caught fish stocks, provides food, protein and a local income to women within the community.
Photography © Positive Change for Marine Life & SISP

subset: commoncrawl


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OMG! Nick Jonas gifted a diamond ring to Parineeti Chopra for 'hiding' his shoes
Wow! We can 't be happier for Priyanka here.Jan 29, 2019, 18:43 PM IST
Will Priyanka Chopra be Meghan Markle's bridesmaid?
Priyanka Chopra stayed tight-lipped about the ceremony on May 19 but was vocal about her belief that Markle will help modernise the British monarchy.Feb 01, 2018, 11:33 AM IST
From bridesmaid to bride for Prince William's sis-in-law Pippa
The 33-year-old married financier James Matthews, 41, at a ceremony attended by the royal couple and tennis star Roger Federer, as she wore a couture dress by British designer Giles Deacon.May 20, 2017, 19:59 PM IST
Wedding season special: Looks trending for bridesmaid this autumn/winter
Whether it is sparkling shagun ceremony or colorful ladies sangeet or the elegant mehndi night or the bright haldi day or the traditional D-day, this is the perfect time for you girls to show-up your fashion side.Nov 22, 2016, 14:46 PM IST
Rihanna turns bridesmaid in assistant's wedding
R&B singer Rihanna turned bridesmaid in assistant Jennifer Rosales' Hawaiian wedding to Aaron Davis. The 27-year-old singer was one of the 10 attendants in the bridal party, reported Us magazine.Apr 22, 2015, 13:14 PM IST
Rihanna becomes bridesmaid for friend
Singer Rihanna was spotted at the wedding of her friend and assistant, Jennifer Morales.Apr 22, 2015, 07:31 AM IST
Lady Gaga serves as bridesmaid at best friend's wedding
Newly engaged singer Lady Gaga served as a bridesmaid at her best friend's wedding in New Orleans.Mar 22, 2015, 15:39 PM IST
Kristen Wigg dating Scott Speedman?
Bridesmaids' star Kristen Wiig is reportedly dating Scott Speedman.Sep 19, 2014, 09:01 AM IST
Snooki makes bridesmaids wear horrible dresses
Reality TV star Snooki wants her bridesmaids to try on horrible dresses for her own amusement.Mar 14, 2014, 19:40 PM IST
Historic movies and comedy interests Jessica Biel
Jessica Biel wants to discover her comic sense by working in a girl comedy like 'Bridesmaid'.Jul 31, 2012, 15:32 PM IST
Naomi Campbell to be Kate Moss` bridesmaid?
When supermodel Kate Moss weds rocker Jamie Hince next month, the nuptials will get an added dose of glamour, in the form of bridesmaid Naomi Campbell.May 17, 2011, 14:30 PM IST
I am the worst bridesmaid in entire world, confesses Keira Knightley
Keira Knightley has confessed that she is the 'worst bridesmaid in the entire world'.May 12, 2011, 18:29 PM IST
Keira plays bridesmaid at brother`s wedding
Kiera Knightley walked down the aisle as the bridesmaid at elder sibling Caleb`s wedding with designer Kerry Nixon.Apr 25, 2011, 18:08 PM IST


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Descubra tudo o que o Scribd tem a oferecer, incluindo livros e audiolivros de grandes editoras.
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http://www.kitchendoctor.com/herbs/artemisia_annua.php http://www.science.gov/topicpages/a/artemisia+annua+plants.html
Taking in consideration that it is one of the deadliest diseases, scientists constantly try to find a cure and finally put and end to cancer. This magical herb is one of those cures that goes unspoken of in the medical community, and it can kill up to 98% of cancer cells in just 16 hours. - See more at: http://www.spiritscienceandmetaphysics.com/amazing-herb-kills-98-of-cancer-cellsin-just-16-hours-shows-multiple-scientific-studies/#sthash.ZqCpuMYn.dpuf Namely, according to a series of studies published in Life Science, artemisinin, a Sweet wormwood or Artemisia Annua derivative, was used in Chinese medicine and it can kill 98% of lung cancer cells in less than 16 hours. The herb used by itself reduces lung cancer cells up to 28%, but in combination with iron, Artemisia Annua successfully and completely erases cancer, and in the experiment this herb had no impact on healthy lung cells. Artemisinin in past was used as a powerful antimalarial remedy, but now it is proven that this cure is also effective in the fight against cancer. When scientists added iron while conducting the study, which later attached to lung tissue, especially to cancer infected cells, artemisinine selectively attacked bad cells, and left good cells untouched.
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In general, our results show that artemisinin stops E2F1 transcription factor and intervenes in destruction of lung cancer cells, meaning it presents a transcription way according to which artemisinin controls reproductive cancer cell growth, was stated in the conclusion of the researches conducted in the cancer laboratory at the University of California. The Journal of Life Science reported on a study out of the University of Washington, conducted by Drs Narenda Singh and Henry Lai. So far, this is the most extensive study done in the US on artemisinin. The study was conducted in a laboratory in tissue samples (in vitro) as opposed to a study on animals or humans (in vivo). Using a radiation resistant variety of breast cancer cells (that also had a high propensity for accumulating iron) artemisinin proved itself to have a 75% cancer kill rate after just 8 hours; and almost a 100% kill rate in just 24 hours. The original study can be found here.
Iron deposits in cancer cells with special receptors which help in cell division. Normal cells also have these receptors, but cancer cells have them in larger amounts and according to this, cancer cells can be a target combination of iron and artemisinin. There are numerous experiments conducted so far and they all prove that in combination with iron, artemisinin can effectively destroy cancer, and this extract was used in China for thousands of years, as a cure for malaria.
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Malaria parasite can not survive in the presence of artemisinine, because it is rich in iron, and bio-engineers Henry Lai and Narendra Singh from the University of Washington were the first scientists to discover this. The original peer reviewed journal article can be found here, where the scientists conclude artemisinin and artemisinin-tagged iron-carrying compounds could be developed into powerful anticancer drugs. Dr. Len Saputo calls artemisinin a cancer smart bomb: - See more at: http://www.spiritscienceandmetaphysics.com/amazing-herb-kills-98-of-cancer-cells-in-just-16-hours-showsmultiple-scientific-studies/#sthash.ZqCpuMYn.dpuf
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Decatur County Memorial Hospital is proud to be a member of Spirit of Women, a network of more than 150 premier hospitals and health systems throughout the United States dedicated to advancing the cause of women's health.
DCMH would like to welcome Kristin Rowland as the new Spirit of Women Coordinator.
Kristin has been a part of Decatur County Memorial Hospital since April 2018, where she started as a health coach. She recently became the Spirit of Women Coordinator in December of 2018. Kristin graduated college in 2011 with a Bachelor's Degree in Kinesiology. She is married and lives in Batesville, IN with her husband Dustin and two children.
Kristin previously worked at St. Andrews Health Campus where she was the Life Enrichment Director. She enjoys spending time with family and friends, exercising, reading, traveling but most of all, being a mom to her two children.
Kristin is excited about this new position and working with Spirit of Women. She added, "Spirit of Women is such a great opportunity for women of all ages to meet others, gain knowledge, and grow as a person. I am looking forward to a fun and exciting year!"
The Spirit of Women program promotes Decatur County Memorial Hospital as the regional leader in women's health education and community outreach. Spirit of Women is a national movement for women's wellness led by local leading hospitals such as Decatur County Memorial Hospital and supported by corporate partners and national media with the mission to motivate women and their families to make positive changes in their lives by emphasizing their total well-being.
The Spirit of Women Membership Program at Decatur County Memorial Hospital gives its members the opportunity to enjoy exclusive benefits, including:
• Free or discounted admission to educational events
• Referral information
• First notice of our upcoming events
• First notice of new health information programs offered at DCMH
• A Spirit of Women magazine
Some of these events include:
• Annual Health Symposium
• Breast Cancer Awareness/Mammography event
• FREE Lunch & Learns throughout the year
If you would like to become a member, please call 812-663-1325 or email email@example.com. You can also go to their website at dcmh.shghealth.com and join online for a one-time/lifetime membership fee of only $10! DCMH Spirit of Women empowers women to take control of their health.


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I have found over the years that martial arts can affect one on a very deep level. When teaching or training in the martial arts one need to be very careful not to hurt anyone with malice. When you live and train with hate or fear in your heart the many problems can happen in your life. One must allow martial arts to clear your heart and free it from the grip of insecure way of living. By training in the correct manner your martial arts method will help you be come very powerful compssionate warm person. Your martial method will only dispense it self when it in needed not because of lack of compassion and insecurety.
Mark Davis
Boston Martial Arts Center


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Sometimes we can be funny. And sometimes we can be serious. In this post, we tried to be both. This post is about some expectations people have with their sessions. So we are here to break it down for you! We hope you enjoy, get a laugh, and also take some things away! So without further ado, the expectations and realities of a boudoir session.
Expectation: I'm going to look and feel sexy while doing it!
Reality: Aaaaahahahahaha, no you won't. You're going to be thinking to yourself, "There is no way this looks good. There is no possible way. I'm shaking like a leaf, my mouth is hanging open, my neck is twisted funny, am I cross eyed?!?" But you just gotta have faith in us. You've seen the proof, it's magical! It just doesn't feel magical in the process.
Expectation: It's going to be easy!
Reality: Oh, babe… it's… a lot of work. You're going to get sweaty, you're going to get shaky in poses, you're going to be sore (even those of you who think, "Psh, ok…"). Think of yoga, now amp it up a bit, now amp it up a bit more. You're stretching SO many muscles in your body. We tell you it feels like an intense work out because it is.
Expectation: I just have to lay there!
Reality: Nopity nopity no. You have to arch, bend, reach, stretch, squeeze. We're going to put your body through a lot but just remember it's out of love! We want you to have the best experience and that means we have to mildly torture you. Don't let it scare you off, it's not as bad as Katie makes it sound and the final result is so so worth it!!
Expectation: They'll photoshop that away!
Reality: Wrong. We don't photoshop. We remove anything that isn't permanent like bruises or blemishes (if you've got a wicked killer bruise though, we might reschedule you because you're probably in pain and we don't want to push you when you're already hurting). You need to see you how others see you. This is not about contorting yourself into something you aren't. This is about starting or continuing your body love journey. Your body does not need to be photoshopped or fixed. It's a body and it's doing exactly as intended.
Expectation: I'm too awkward!
Reality: Human, Jes will tell and show you how to do everything - including how to breathe!! You will not have the ability to be awkward. Once you make it through your first pose, you will be a damn natural and that thought will go right out the window!
Expectation: I don't wear or own lingerie, I can't do this!
Reality: I personally don't know anyone who just hangs around their house in a teddy. The typical cycle of owning lingerie goes something like {I bought this super cute thing, wears super cute thing for five minutes, tucks it away in a drawer to keep it safe, run out of clean underwear and wear the super cute thing, put super cute thing in rotation, eventually throw super cute thing away}. The cool thing about our closet is that most of it is actually NOT lingerie. We have dresses, leggings, bodysuits, crop tops, jackets, robes SO MANY THINGS!!
Expectation: Jes can never make me look that good!
Reality: LIAR!! As Jes says on the regular, "Did you really think you were the one person I can't make look good?!" You are a damn glorious unicorn, in every damn way, and yeah, you do look that damn good.
Expectation: I want to be girl of the week but I have to fill out this questionnaire that doesn't work for me!
Reality: Don't like the questionnaire? It isn't as in depth as you would like it to be? That's cool! Use your words, tell your story in your own way! Your experience is one of a kind. If you're worried about grammar or spelling or that you can't write, I assure you, that's what Katie is here for. You can work one on one with her or you can send her something and she can fix it up for you! Whatever you want!!
We hope you learned something, had some fun, and that we eased some of your fears. Are you a past client and we missed something? Tell everyone in the comments! Sharing knowledge is beneficial to all of us, so tell us what you got!!
Have a great day, mermaids!!


text: Bridging the Human/Machine Gap with Augmented Reality Oct 16, 2019 The industrial internet of things and augmented reality are driving the convergence of humans and machines to usher in the collaborative... Webinar: Industrialize Your Data to Deliver Industry 4.0 Oct 16, 2019 A November 14 IndustryWeek-hosted webinar sponsored by MarkLogic Webinar: Realizing your Manufacturing Digital Twin Oct 16, 2019 An On Demand IndustryWeek-hosted webinar, sponsored by Dassault Systèmes IW eBook: Robots, Humans and the Future of Work In this exclusive collection, we tackle the full automation story -- from the fears of job-killing robots, to operations and efficiency, and to developing new business strategies. Download Now Sign up for the IW Daily Headlines newsletter. AfghanistanAland IslandsAlbaniaAlgeriaAmerican SamoaAndorraAngolaAnguillaAntarcticaAntigua and BarbudaArgentinaArmeniaArubaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermudaBhutanBoliviaBosnia and HerzegovinaBotswanaBouvet IslandBrazilBritish Indian Ocean TerritoryBritish Virgin IslandsBruneiBulgariaBurkina FasoBurundiCambodiaCameroonCanadaCape VerdeCaribbean NetherlandsCayman IslandsCentral African RepublicChadChileChinaChristmas IslandCocos (Keeling) IslandsColombiaComorosCongo (Brazzaville)Congo (Kinshasa)Cook IslandsCosta RicaCroatiaCubaCuraçaoCyprusCzech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFalkland IslandsFaroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaFrench Southern TerritoriesGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuamGuatemalaGuernseyGuineaGuinea-BissauGuyanaHaitiHeard Island and McDonald IslandsHondurasHong Kong S.A.R., ChinaHungaryIcelandIndiaIndonesiaIranIraqIrelandIsle of ManIsraelItalyIvory CoastJamaicaJapanJerseyJordanKazakhstanKenyaKiribatiKuwaitKyrgyzstanLaosLatviaLebanonLesothoLiberiaLibyaLiechtensteinLithuaniaLuxembourgMacao S.A.R., ChinaMacedoniaMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMartiniqueMauritaniaMauritiusMayotteMexicoMicronesiaMoldovaMonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNetherlands AntillesNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorthern Mariana IslandsNorth KoreaNorwayOmanPakistanPalauPalestinian TerritoryPanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalPuerto RicoQatarReunionRomaniaRussiaRwandaSaint BarthélemySaint HelenaSaint Kitts and NevisSaint LuciaSaint Martin (French part)Saint Pierre and MiquelonSaint Vincent and the GrenadinesSamoaSan MarinoSao Tome and PrincipeSaudi ArabiaSenegalSerbiaSeychellesSierra LeoneSingaporeSint MaartenSlovakiaSloveniaSolomon IslandsSomaliaSouth AfricaSouth Georgia and the South Sandwich IslandsSouth KoreaSouth SudanSpainSri LankaSudanSurinameSvalbard and Jan MayenSwazilandSwedenSwitzerlandSyriaTaiwanTajikistanTanzaniaThailandTimor-LesteTogoTokelauTongaTrinidad and TobagoTunisiaTurkeyTurkmenistanTurks and Caicos IslandsTuvaluU.S. Virgin IslandsUgandaUkraineUnited Arab EmiratesUnited KingdomUnited StatesUnited States Minor Outlying IslandsUruguayUzbekistanVanuatuVaticanVenezuelaVietnamWallis and FutunaWestern SaharaYemenZambiaZimbabwe Sign Up Enter your email above to receive messages about offerings by Informa, its brands, affiliates and/or third-party partners, consistent with Informa's Privacy Statement. List Leave this field blank Webinar: How to Leverage Edge Controllers to Enhance Your Operations and Drive Savings Sep 16, 2019 An October 31 IndustryWeek and Machine Design-hosted webinar, sponsored by Emerson Is Messy Data An Inevitability? Aug 05, 2019 Let's push back a little. Staying Secure in the IIoT Patchwork Jul 02, 2019 Have a consistent plan in place and follow it with the help of these three steps. Getting Your IIoT Project out of Pilot Purgatory Jun 24, 2019 These 10 recommendations will help counteract the inertia. 8 Reasons Why Your IIoT Project Is Stuck in Pilot Purgatory Jun 21, 2019 84% of companies working in IoT are stuck in pilot mode, 28% of them for over two years. Your IIoT Questions, Answered May 15, 2019 A tech expert at Kuka addresses some basics. Webinar: Moving Manufacturing Beyond Lean with Digitalization and IIoT May 01, 2019 An On-Demand IndustryWeek-hosted webinar, sponsored by AutomaTech and GE Digital The Know-Your-Enemies Approach to Cybersecurity Apr 22, 2019 In an environment where attack surfaces are expanding thanks to newly connected OT devices, staying one step ahead of the bad guys is critical. Ask: Who Else Can Use This Data? Apr 08, 2019 The attitudinal shift vital to full IIoT transformation Winning with IoT Apr 05, 2019 In IBM Institute study, manufacturing innovators share ways they make data work. Load More 1 2 3 4 5 next last Load More


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Erica McNab, teacher
Humans of Esperanza
March 10, 2019
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"I have never met someone who sees me the way [my fiancé] does. He sees me in the most genuine, real way. He understands me in a way no one else does. He knows what I'm thinking before I think it. He knows what I need before I say it, and is just my best friend on every level. I see him and he sees me with no walls or allusions, just completely genuine and loyal, there unapologetically."


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5 Key Topics Important to Consumer Bankers
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Meet with Treliant at MBA's Regulatory Compliance Conference - 2018
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Articles and Insights
September 13, 2019
US Regulators Reinforce the Importance of Risk Assessments - Efren Marquez Alba
July 08, 2019
Reg. BI, What You Need to Know to Prepare for Implementation - Gino Ercolino
May 09, 2019
OFAC and U.S. DOJ Reinforce Requirements for Sanctions and Corporate Compliance Programs - Ross Marrazzo and Ron Ziegler
March 01, 2019
The Dark Side of Vendor Management - Susanna Tisa
February 22, 2019
Sales Practice Risk in the Digital Era Part 2: Mitigating Sales Practice Risk - Greg Soueid, Lynn Woosley, and Dominic Mangonon
February 20, 2019
Compliance in 2019: What to Expect, and Where Should Your Focus Be? - Carl G. Pry
February 15, 2019
Sales Practice Risk in the Digital Era, Part 1: Where We Are, Where We're Going - Greg Soueid, Lynn Woosley, and Dominic Mangonon
December 12, 2018
Ross Marrazzo Featured in a Podcast at the ABA/ABA Financial Crimes Enforcement Conference
December 10, 2018
Hidden Tripwires in the new 2018 HMDA Rules - Carl G. Pry
December 03, 2018
Lynn Woosley Quoted by Asurity Technologies
November 29, 2018
Despite Uncertainty, Protections for Military Borrowers Remain a Priority - April A. Breslaw
November 28, 2018
Data Privacy Policy at a Crossroads - Agnes Bundy Scanlan and Ash Khan
October 15, 2018
Effective Reporting Can Support Improved Decision-Making by Directors and Executives - Ross Marrazzo
September 05, 2018
Mitigating Compliance Officer Risk—the Making of a Good Compliance Officer - Ross Marrazzo and John P. Carey
July 18, 2018
Bending rules isn't worth the risk – B. Scott Fisher
June 01, 2018
Warning: Outsourcing May Be Hazardous to Your Health - Ross Marrazzo
May 02, 2018
Ask Treliant: Best Practices for Conducting Risk and Control Self-Assessments (RCSAs) - Janet M. Hale, Lynn Woosley, and Timothy J. Stokes
April 18, 2018
Is Your Bank Ready for GDPR? - Agnes Bundy Scanlan
March 27, 2018
Control Digital Transformation's Regulatory Risks - Lynn Woosley and Ellen Rose
March 23, 2018
Best Practice in Risk Management: Right-thinking and Right-sizing the Three Lines of Defense - Graham A.D. Broyd and Catherine Cullen
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Friday, November 7, 2008
It All About Me............
Yesterday in media literacy class I added a video bar to my blog page. what did artistes that I chose to be on my video bar say about me........well it more than likely gave the impression that my favorite music is hip-hop and r&b and it also suggests that I like a little of pop and rock.
Posted by Tiana Hope at 9:21 AM
1 comment:
- Eb the Celeb said...
I read threw a couple posts and I am loving your media literacy class...
- November 11, 2008 at 1:37 PM
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|» Products
|» Home Lift
|» Home traction collapsible Door
Home traction collapsible Door
Product Code : SRL-HTCD-10
Brand Name : axel srl
Price And Quantity
- Minimum Order Quantity
- 1
- Unit of Measure
- Piece/Pieces
- Price
Product Specifications
- Machine Room Size
- As per Requirement
- Hoist Way Size
- As per Requirement
- Noise Level
- 0.01 db
- Usage
- Building Elevator, For Passengers Loading, Residential Elevators
- Load Capacity
- 408 Kilograms (kg)
- Door Opening Size
- As per Requirement
- Pit Depth
- 1500 Millimeter (mm)
- Type
- Passenger Elevators
- Car Dimension
- As per Requirement
- Overhead Height
- 1400 Millimeter (mm)
- Material
- Steel, Stainless Steel, Iron, other Sheet, Rubber, Plastic, Bronze, Brass, Zinc, Stainless Steel, Galvanized Steel, Copper, Steel, Aluminum Alloy
- Drive Type
- Power Supply
- Speed
- 1.0 m/s
- Max. Lifting Weight
- 408 Kilograms (kg)
- Mechanism Type
- Rack-and-Pinion Elevator, Belts And Belt Elevators
- Control Device
- Calling Box
- Safety Device
- Down Over Speed Protection Device, Repeated Door Closing, Fault Self Diagnosis, Anti-stall Timer Protection, Overload Holding Stop, Designated Stop, Photocell protection, Auto-Flap, Emergency Lowering, Safety Sensor
Product Description
Home Traction Collapsible Door is perfect to be installed in residential societies for being cost effective & completely reliable in nature. Cabin of this lift can accommodate up to 6 people and is provided with cast iron made collapsible manual door. It does not launch until door is completely closed hence assures utmost safety. This lift operates with help of traction system and is equipped with integrated brakes for added safety. It is also provided with overload sensor that prevents launching until it is within permissible weight limit. Home Traction Collapsible Door is ideal for building with four floors and requires low maintenance.
Collapsible Door-Mild Steel collapsible door,
Capacity-6 passenger.
Trade Information
- FOB Port
- Kolkata
- Payment Terms
- Cash in Advance (CID)
- Supply Ability
- 1 Per Day
- Delivery Time
- 1 Week
- Main Domestic Market
- West Bengal
Other Products in 'Home Lift' category
"We Mainly Deal in East India Zone."
- Contact Details
- Plot no. 2D/31/1, Street No. 1111, Action Area II, New Town, Rajarhat, Kolkata - 700156, West Bengal, India
- Phone : +918068213228
- Mr Tanmay Biswas (Partner)
- Mobile : +917890053878
- Mr. Subhra Sankha Roy (Partner)
- Mobile : +918068213228
Accepts only Domestic inquiries Send Inquiry
- Reg.Office: Street No 100, Block No: AB, Newtown, Kolkata: 700156.
AXEL SRL ELEVATOR & ENGINEERING All Rights Reserved.(Terms of Use)
Developed and Managed by Infocom Network Ltd.


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Imrpove your Summer look with this gorgeous maxi dress in beige color. Love the strapless design and the divided skirt. Complete it with metallic red ankle cuff sandals and embroidered oversized sunglasses.
Tag: Red Sandals
Frayed Jeans In Wash Blue And Red Heeled Sandals For Summer Casual Parties
How to make your denim look fresh, individual and special? We see a pair of frayed jeans in wash-blue styled with hot fiery red heeled sandals. A lovely combination for young ladies who want to underline their individuality. Wear these jeans and red heeled sandals to casual street walks or to roof parties.
Can I Wear Off-Shoulder Ruffled Shift Dress This Summer
Have a wonderful Summer day by wearing one of these sexy off-shoulder shift dresses. I do love a relaxed touch of these dresses and the way they are completed with red sandals. Make sure to complete these dresses with some classy sunglasses.
How To Wear Red Sandals This Summer
Get inspired by these two looks where young ladies appear in red heeled sandals styled with off-shoulder shift dresses with ruffled details. Both outfits look pretty sweet and classy. Would you try them on?
With What To Wear Suede Heeled Sandals This Summer
If you want to underline your femininity, then you definitely should give a try to these suede heeled sandals. I am a big fan of suede, that's why I do recommend to wear it. We see a pair of red sandals with block heels and two sandals in black color. Check out the ones in black net, they are so special and sophisticated. Which one is your favorite?
Continue reading With What To Wear Suede Heeled Sandals This Summer


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Merchant Focus Overview
Founded in 2004
Merchant Focus, also known as "Established Merchant Focus," is a small to mid-sized merchant account provider based out of Dublin, Ohio. The company was established in 2004 and appears to focus solely on providing credit card processing services. Merchant Focus also has a sister company called "Established Merchants Only" that serves businesses with over $250,000 in credit card sales per year.
A Joint Venture Operation
Merchant Focus utilizes Authorize.net for its payment gateway and e-commerce processing services. Merchant Focus was founded by the two co-founders of e-onlinedata, which they sold in 2007 to PowerPay (now EVO Payments International). The founder of PowerPay, Steve Goodrich, is now a partner at Merchant Focus. Merchant Focus also acts as the processor for the mobile credit card processing services Inner Fence and AppNinja's Swipe.
Merchant Focus Location and Ownership
The acquiring bank of Merchant Focus is Deutsche Bank AG, New York. John Waldron is listed as the CEO and Jason McLeod is the president of Merchant Focus, which maintains its headquarters at 105 North Riverview, #314, Dublin, Ohio 43017.
Merchant Focus Review Table of Contents
- Costs & Contract: Merchant Focus appears to offer a month-to-month agreement with no early termination fee.
- Complaints & Service: Merchant Focus has received fewer than 10 public complaints.
- BBB Rating: Merchant Focus has an "A+" rating and has received 0 complaints and 1 review in the past 3 years. The company has been accredited by the Better Business Bureau since 2006.
- Sales & Marketing: Merchant Focus appears to hire independent sales representatives but has not received any complaints related to its sales team.
- Rates & Fees: How Merchants Got The Best Rates With Merchant Focus
Merchant Focus Statement Analyzer
Find hidden fees, pricing errors, and overcharges in your Merchant Focus merchant account statement. Not with Merchant Focus? That's ok, we can find excessive fees in any credit card processing statement. We've already found $1,156,834 in hidden fees this year! Submit a recent statement to try it out for free!
Our unbiased reviews are supported, in part, by helping you find highly rated merchant accounts. You can learn more here.


text: Support 241 #1 Posted October 12, 2018 WASHINGTON, D.C.—The death toll from Justice Brett Kavanaugh's first week on the Supreme Court has just topped 330 million, a report from the CDC indicated Friday. The post Death Toll From Kavanaugh's First Week On Supreme Court Tops 330 Million appeared first on The Babylon Bee. View the original full article Share this post Link to post Share on other sites


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It's hard to find something more quintessentially British than rain. Tackle the inevitable with style by using our Telescopic Umbrella to protect you from the elements.
Telescopic Umbrella
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Location: Building 2120, Room 126
Hours: Monday through Friday, 8:30 a.m. to 4:30 p.m.
Telephone: 509-247-5406
About us: Our clinic familiarizes the flyer or jumper with the physiological stresses and human performance factors of modern military aviation and prepares them to successfully meet these challenges. Our goal is to enhance flight safety by reducing human performance errors. We provide Hypoxia Familiarization Training via the Reduced Oxygen Breathing Device. The HFT/ROBD is the most advanced, effects-based hypoxia training available and is an alternative to the altitude chamber. This training satisfies AFI 11-403 requirements for aircrew physiology training and offers the same five year currency for: Tanker, Transport, and Bomber, aircrew Refresher; High Altitude Parachutist Initial and Refresher.
Location: Building 9000, Rm 225
Telephone: 509-247-4494
Fax: 509-247-8833
About us: We provide annual Preventive Health Assessments for non-flyers, occupational health physicals, pre-employment physicals, retraining physicals, medical overseas clearances and separation health physical exams. For non-PHA related issues, please call 509-247-2534 or 509-247-4494.
Location: Building 9000, Room 130
Telephone: 509-247-2391
About us: Our primary mission is to provide health risk assessment and mitigation recommendations to commanders in support of 92 ARW and tenant unit operations. We provide health surveillance for Team Fairchild personnel through sampling, identification, quantification, assessment, risk communication, control and documentation of potential exposures. Any Team Fairchild member with an occupational or environmental health concern may contact BEF and request an assessment. In addition, we plan, prepare for, and provide real time CBRN response, ensures safe potable and non-potable water, and performs specialized vulnerability assessments for drinking water, toxic industrial chemicals and toxic industrial materials.
Location: Building 9000, Room 125
Telephone: 509-247-4343
Fax: 509-247-5993
About us: No appointments necessary. All labs require an order from your primary care manager or civilian physician.
Location: Building 9000, Room 333
Hours: Monday through Friday, 7:30 a.m. to 4:30 p.m.
Telephone: 509-247-3609, DSN 657-3609
Fax: 509-247-2170
About us: We provide administrative support to all members of the 92 MDG staff.
Location: Building 9008
Hours: Monday through Friday, 7:15 a.m. to 4:30 p.m.
Telephone: 509-247-5820
Fax: 509-247-4140
About us: Services include: annual exams; cleanings; preventive; routine restorative; oral surgery and emergent care. For an after-hour dental emergencies, such severe pain, bleeding, swelling, for which you feel you need to speak with a dentist during non-duty hours, call the Dental Officer on duty at 509-994-9637.
Location: Building 9000, Room 132
Telephone: 509-247-2617
Fax: 509-247-4336
About us: We provide routine X-ray exams for all eligible beneficiaries. All other imaging services are referred off-base to the local network. Exams are performed on a walk-in basis with no appointment necessary. Prescriptions for x-rays from approved Health Net Federal Services providers are accepted. Exams requested by off-base chiropractic clinics must be approved and ordered by a 92nd Medical Group Provider. Diagnostic Imaging can provide patients with copies of their exams on a disk. Patients can receive a CD by requesting in person at the radiology front desk. Radiology staff will require the patient to present their ID card. Diagnostic Imaging cannot release or discuss results with patients. Please refer to the ordering provider or Medical Records for results.
Location: Building 9000, Room 339
Telephone: 509-247-5574 or 509-247-2268
Fax: 509-247-4936
About us: We identify and assist with medical and educational service requirements for active duty family members and dependents, while also ensuring administrative process requirements are met for family member relocations and PCS's. Can also assist members with the Exceptional Family Member Program reassignment process, as necessary. EFMP is designed to provide support to military family members with special needs. All branches of the military offer EFMP, and each service includes a variety of personnel, medical, and family support functions under the EFMP umbrella. Learn more about the Exceptional Family Member Program.
Location: Building 9000, Second Floor
Telephone: 509-247-5755
About us: We are dedicated to providing exceptional medical care to our flying population, special duty personnel, and their family members. Active flyers requiring a return to flight status will report to Flight Medicine at 7:30 a.m.
Location: Building 9000, Room 106
Hours: Monday through Friday, 7:30 a.m. to 3:30 p.m.
Telephone: 509-247-4108
Fax: 509-247-1300
About us: The TRICARE beneficiary advocate ensures health benefit information and assistance to access health care is available to all eligible beneficiaries. They also assist beneficiaries with debt collections and adverse credit ratings due to an unpaid TRICARE bills. Patients should never ignore a bill assuming TRICARE will handle it automatically. If a patient receives a bill, they must call the doctor's office or hospital that issued the bill. Typically, the number is listed on the bill. If that does not resolve the bill, the patient should then call the TRICARE contractor, which is currently Health Net Federal Services in the West Region. If the bill is still not resolved, the patient may report to or call the Beneficiary Counseling & Assistance Coordinator or Debt Collections Assistance Officer for assistance.
Telephone: 509-247-5757
About us: To schedule a hearing test, please contact Public Health at 509-247-5757.
Location: Building 9000, Rm 243
Telephone: 509-247-5114
Fax: 509-247-2539
About us: We provide comprehensive eye exams to active duty personnel, TRICARE Prime beneficiaries, and individuals with TRICARE Plus, Standard, and TRICARE For Life, and can be seen on a space available basis. Appointments can be made by calling the Central Appointment Line at 509-247-2361.
Location: Building 9000, Room 109
Telephone: 509-247-5520
Fax: 509-247-5925
About us: We maintain outpatient medical records for all non-flying active duty personnel, family members, and retirees and family members. Registers patients in MHS GENESIS.
Location: Building 9000, Room 110B
Telephone: 509-247-3392 or 509-247-5520
About us: We assist patients and non-medical attendants with the travel order and reimbursement process through the Defense Travel System.
Location: Building 9000, Room 344
Telephone: 509-247-9220
Fax: 509-247-4274
About us: We serve as a liaison for patients entering and undergoing medical evaluation board process, as well as coordinates with Veterans Administration and Air Force Personnel Center on behalf of the patient.
Location: Building 9000, Room 101
Telephone: 509-247-9487
Fax: 509-247-2057 or 509-247-2681
About us: We educate patients on how to use referrals issued by primary care managers and assists in resolving referral issues.
Telephone: 509-247-5758
About us: We release medical information to third parties for treatment, payment, operations, etc. Patients will visit this section to request their medical records be sent to the specialty providers they are referred to by their primary care managers. Processes requests for patient copies of medical records.
Location: Building 9000, Room 357
Telephone: 509-247-3300
About us: We are responsible for effective management of medical resources. RMO is accountable for: reimbursements to include other health insurance, third party liability, and all other collections; accounts for all manpower and timecards within the facility and determines future staffing; and approves orders and vouchers for patient and staff travel. Hospital data is collected in our data quality office and used to establish more encompassing care for our beneficiaries. The funding for the daily operations of this hospital is made possible by our cost center manager program. You are welcome to stop by to update your OHI, pay medical bills, or ask questions.


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(Image: Courtesy of Eden Boy's School)
Pupils from Eden Boys' School took part in a slumber party with a difference, in aid of homelessness charity St Basils.
Eight pupils from Eden Boys' School and four from Eden Boys' Leadership Academy built beds out of cardboard and plastic sheeting; bedding down in the grounds of the Birmingham Cathedral.
The efforts for the Big Birmingham Sleepout raised £100 for St Basils, a charity that houses more than 1,000 young people across the city and more than 1,500 across the West Midlands. It also helps thousands more with face-to-face support.
Year 9 pupil Salahuddin Khan said: "After a night of surviving the cold, I realised what it was like to have no food or no water, and how it felt living on the streets of town. I believe more could be done to help the homeless and that more should be done to ensure that they are taken good care of. If we continue to raise awareness and take part in events like the sleepout, I hope that the vast number of people sleeping rough on the streets is reduced."
Pupils will also take part in the 'Sheets not Streets' programme during the Christmas period too, which will see them donate brand-new bedding to the charity.
Asiyah Ravat, Principal at Eden Boys' School, Birmingham, said: "It was very, very cold so nobody had much sleep that night. As a result, I really think it hit home with the boys how difficult it would be to be a rough sleeper, especially at this time of the year and made them realise how privileged they are to have had a warm home to return to."
For further information on the charity St Basils visit www.stbasils.org.uk


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Two folks who are kinda awkward (or shy) with first dates make a show about first date songs. Amy makes the worst singles ad after tripping over her own words and Mitchell declares that he is slightly shy (what?). This show reveals all the Earbuddies' first date tactics and feels but also gives a huge variety to add to that first date playlist!
Thank You, Kaylah, for the ideas list!
Voicemail: 731-400-BUDS(2837)
Email: email@example.com
The Neighbourhood - Sweater Weather
The Cure - Friday I'm In Love
Turnover - Dizzy on the Comedown
Ratboys - Charles Bernstein
Sleepwalkers - In My Dreams
Seam - Rafael
Jane's Addiction - Classic Girl
Pulp - Something Changed
Matthew Sweet - Girlfriend
The Replacements - I Will Dare
Seratones - Gotta Get to Know Ya
Ellis Paul - Drive In Movie


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This ability innovate is becoming increasingly important. Consider the challenges that we face today, exemplified by the United Nation's Sustainable Development Goals: climate change, food- and bio-security, terrorism, population growth, health care. These and many other challenges are complex and interconnected – they are not within a single scientific discipline and they cross many geographies, cultures, politics. What's more, is that many of them are a result of the rapidly growing human population and consumption, and they are impacting every species on the planet. Paul Ehrlich and Robert Pringle have written that 'the fate of biological diversity for the next 10 million years will almost certainly be determined during the next 50-100 years by the activities of a single species' – humans.
Another way of putting it, there are thought to be over 8,700,000 species on Earth, and absolute decision making authority is invested in only one species. To solve these challenges will require a significant mobilisation and sharing of people, knowledge, and resources and no single person, organisation, or country can solve them. These challenges fundamentally different to those we have faced in the past. Our methods and approaches need to evolve just as the challenges have. We need a new type of Innovation. We need Innovation:Amplified.


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Beauty by Hong is an expert-driven beauty salon in Adelaide that operates and delivers to its customers the latest beauty trends like Microblading, Feathering and semi-permanent makeup in Adelaide at an affordable price. Our cosmetic tattoo and eyelash extension in Adelaide adhere to the latest technology so that our customers can get the most updated treatment for beautification.
Additional Info
|Appointment Required


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The Shepherd's Voice
with Archbishop George J. Lucas
We found 2 episodes of The Shepherd's Voice with the tag "advent".
Episode 24: Christmas: Jesus Wades into the Human Mess
Episode | December 21st, 2018 | 21 mins 3 secs
advent, hope
In the face of challenges - those facing the Church, those facing us personally - it's easy to feel that God is distant from us. What the Church proposes, however, is that the Lord is near and that He enters into our particular circumstances. This time of year, our primary task is to ask for Him to come near, and to be open to how He wants to make Himself known to us.
Episode 7: This Advent, "Watch!"
Episode | December 1st, 2017 | 9 mins 20 secs
advent, catholic, christmas, faith
Preparing for Christmas often feels more like a project than anything else. The challenge to our faith during this season is to think about what God has done and is doing. We remember that we're saved because of God's project, God's work, God's action in our lives, and not by our own efforts.


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Previously, in our family, a situation often arose when I wanted to pamper my husband and daughter with something delicious, and the child did not understand her mother's good intentions and was constantly underfoot. I tried to distract my daughters with toys or a TV, but it did not work. I started to get nervous and did not want to cook at all.
I thought, I thought, and found a wonderful way out: now my daughter and I are preparing delicious food for dad.
Combine, so to speak, pleasant with useful. What is the pleasantness of this action, I think everyone understands. But how is this useful?
Well, firstly, a joint interesting activity unites mother and child even more. Secondly, any cooking for a child is a game that is very useful for developing fine motor skills. And finally, thirdly, the variants of this game can be infinite.
It all depends on the rich imagination of mommy. For example, a child can cut with a knife (childish, of course!) Vegetables for soup, sort out cereals, wash fruits or berries for compote, sculpt dumplings or buns.
As an example, I would like to show how my daughter and I make baby cookies.
The recipe is very simple. So, we will need:
butter – 200 g
sugar – 200 g
sour cream – 300 g
flour – 600 g
soda – 1 tsp
The softened butter (although 200 g is indicated in the recipe, I usually take 100 g, otherwise the fatty biscuit is obtained) rub with sugar.
Add sour cream, soda and mix well.
Sift flour so that it is filled with oxygen. Mix with the rest of the mass.
Knead is not very cool dough. Wrap it in cellophane and put it in the fridge for 30 minutes.
Sprinkle the table with flour and roll out the dough about 5 mm. thick You can sprinkle sugar on top, cinnamon – in general, what your soul desires.
Cut out different dough from the dough. We use molds for modeling of clay (previously washed), and when we get tired, we just make a glass. This is the longest stage: we cut the figures, crush the dough, re-roll and re-cut. It can last from 30 minutes to 1 hour.
Grease a baking sheet with vegetable oil and spread cookies.
We put in the oven, preheated to 180 degrees. We bake about 20-25 minutes until they become golden in color. The main thing – do not overdry.
Done! Cookies get crumbly and fragrant. In general, yummy, as my daughter says.
Then the daughter treats everyone with these cookies: both father and grandparents, who come to visit us from the next door. All praise little cook. A cook is so happy and proud. And sentencing: Ksyusha – well done. She baked cookies and fed everyone!
As you can imagine, taste is not important in our cookies. In this way I teach my daughter to work and independence. Through the game.


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Pikmi Pops item 75191
Style Series Mega Pack
Pikmi Pops are the big lollipops filled with cute plushies and so many sweet surprises for you!Introducing new Pikmi Pops Style Series - a cute surprise that you can wear! This season the Pikmis come with a comfortable elastic band so that you can wear them! Slide your favorite Pikmis on your fingers, clip them onto your jacket or hang them off your bag!This Mega Pack includes 7 sweet scented plush and 7 surprises. There are 2 exclusive Pikmis in each pack. Feeling lucky? You might unbox a bonus light-up DigiWatch!*There are 3 assorted Mega Packs to collect: Coconut, Marshmallow and Frosted Donut.Don't pick one, pick a lot - and unwrap the cute surprises in your Pikmi Pops!*DigiWatches are available in 1 in every 24 Mega Packs.
Pikmi Pops item 75191
Style Series Mega Pack
What's Inside:
- 7 x Scented Plush
- 7 x Dangler Strings
- 7 x Surprise Messages
- 7 x Surprise Items
- 1 x Collector's Guide


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The EGR valve was replaced and it didn't go away. As a temporary measure you can open the radiator cap when cold and fill with coolant while idling the engine. Did you ever have the timing belt or water pump replaced? A wave of water came over my hood and windshield. And it runs perfectly. I don't recommend driving it if you can avoid it. So i just switched out the ICM from the distributor and still didn't make a difference, next to check injectors and pray thats the problem...
Last edited by Geonerd on Mon Jun 06, 2016 3: He probably didnt test all of them again.
But I have the same symptoms. No new notifications! The O2 sensor was mounted on a little flat metal stem off the intake manifold, and some oil from an iffy valve cover gasket had seeped down the underside of the stem and found its way into the O2 sensor, fouling it.
Shipping Schedule. Sensor was changed about 4 years ago.
I have no idea heard it could b plug wires. The recommended a local garage. That continued until I got home.
Attempted remedies: Please refer to CarGurus Terms of Use. NO chance. I have honda civic 2003 1.
I explained what I have done so far. It's a five speed manual trans.
Good luck. Not even the coil was bad.
Turns out the spark plug wires are bad even though they are only a few years old.


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Human carbonic anhydrase XII: cDNA cloning, expression, and chromosomal localization of a carbonic anhydrase gene that is overexpressed in some renal cell cancers
Contributed by William S. Sly
We report the cloning and characterization of a tumor-associated carbonic anhydrase (CA) that was identified in a human renal cell carcinoma (RCC) by serological expression screening with autologous antibodies. The cDNA sequence predicts a 354-amino acid polypeptide with a molecular mass of 39,448 Da that has features of a type I membrane protein. The predicted sequence includes a 29-amino acid signal sequence, a 261-amino acid CA domain, an additional short extracellular segment, a 26-amino acid hydrophobic transmembrane domain, and a hydrophilic C-terminal cytoplasmic tail of 29 amino acids that contains two potential phosphorylation sites. The extracellular CA domain shows 30–42% homology with known human CAs, contains all three Zn-binding histidine residues found in active CAs, and contains two potential sites for asparagine glycosylation. When expressed in COS cells, the cDNA produced a 43- to 44-kDa protein in membranes that had around one-sixth the CA activity of membranes from COS cells transfected with the same vector expressing bovine CA IV. We have designated this human protein CA XII. Northern blot analysis of normal tissues demonstrated a 4.5-kb transcript only in kidney and intestine. However, in 10% of patients with RCC, the CA XII transcript was expressed at much higher levels in the RCC than in surrounding normal kidney tissue. The CA XII gene was mapped by using fluorescence in situ hybridization to 15q22. CA XII is the second catalytically active membrane CA reported to be overexpressed in certain cancers. Its relationship to oncogenesis and its potential as a clinically useful tumor marker clearly merit further investigation.
The growing carbonic anhydrase (CA) gene family includes nine enzymatically active CAs and three acatalytic CA-related proteins (CA-RPs). The active CAs catalyze the reversible hydration of CO2 in the reaction CO2 + H2O ⇄ HCO3− + H+ (1–4). The CA isozymes differ in their kinetic properties, their tissue distribution and subcellular localization, and their susceptibility to various inhibitors. They play important roles in diverse physiological processes including respiration, bone resorption, renal acidification, gluconeogenesis, signal transduction, and formation of cerebrospinal fluid and gastric acid (1–5). The CA-RPs are membrane proteins that contain a CA domain, but have amino acid substitutions in one or more of the three Zn-binding histidine residues in the active site of catalytic CAs (6). Although the CA-RPs are acatalytic in terms of CA activity, they show much higher sequence identity between mouse and human than the catalytic CAs, suggesting that each of them has an important function, nonetheless. An association of a CA domain with cancer was provided by the discovery that a complete, acatalytic CA domain forms the ligand-binding domain for each of two members of the receptor protein tyrosine kinase phosphatase (RPTP) family (RPTPβ and RPTPγ), one of which (RPTPγ) has been suggested to be a tumor suppressor gene (7, 8). More recently, a tumor-associated marker protein called MN, which was initially cloned from HeLa cells (9, 10), was found to contain a complete CA domain in the middle of its large extracellular segment and to have CA activity when the recombinant protein was expressed (11). The MN protein, which has since been renamed CA IX, was found to be expressed in tumors of several tissues in which it is not expressed normally (12) and also expressed in normal stomach (13) and proliferative enterocytes of the gut (14). Further evidence that CA IX is related to oncogenesis was provided by the observations that its expression in NIH 3T3 cells led to loss of contact inhibition, a shorter doubling time, a decreased dependence on serum growth factors, and loss of anchorage dependence as evidenced by ability to grow in soft agar (10).
We have discovered still another catalytically active CA associated with human cancers. A transcript was identified in mRNA from a human renal cell cancer (RCC) that turned out to encode a human membrane CA that was overexpressed in the clear cell RCC. In this report, we present the identification, cloning, and characterization of this CA, which we have designated CA XII
Sera and Tissues.
The study has been approved by the local ethical review board (Ethikkommission der Ärztekammer des Saarlandes). Recombinant DNA work was done with the official permission and according to the rules of the state government of Saarland. Sera and tumor tissues were obtained during routine diagnostic or therapeutic procedures. Sera were stored at −80°C until use. Normal tissues were collected from autopsies of tumor-free patients.
Construction of cDNA Expression Libraries.
The construction of the RCC cDNA expression library has been described elsewhere (15). In brief, a cDNA expression library resulting in 1 × 106 primary clones was established by directionally cloning cDNA derived from the RCC of a 69-year-old woman with an RCC of clear cell type into the EcoRI- and XhoI-digested ZAPII phage (Stratagene).
Immunoscreening of Transfectants.
The immunoscreening for the detection of clones reactive with IgG antibodies in the 1:100-diluted autologous serum was described (15). Briefly, after transfection for primary screening and plaque transfer onto nitrocellulose membranes (Sartorius) and blocking with 5% (wt/vol) low fat milk Tris-buffered saline (Glücksklee, Nestlé, Vevey, Switzerland), nitrocellulose membranes were incubated overnight in 1:300-diluted autologous patient's serum extensively preabsorbed against Escherichia coli proteins. An alkaline phosphatase-conjugated antibody specific for human IgG (Dianova, Hamburg, Germany) was used to visualize reactive clones.
Sequence and Structure Analysis of Identified Antigens.
Clones reactive with high-titer IgG antibodies were subcloned and submitted to in vivo excision (18) of pBluescript-phagemides (19). The nucleotide sequence of cDNA inserts was determined by using a Sequenase 2.0 kit (United States Biochemical). Initial sequencing was performed according to the manufacturer's instructions using vector-specific reverse and universal primers. Subsequent sequencing was carried out with internal oligonucleotides. Sequence alignments were performed with dnasis (Pharmacia Biotech) and blast (20) software and on EMBL (21), GenBank (22), PROSITE (23), and TMpred (24) databases.
Cloning of Full-Length Transcript by Rapid Amplification of cDNA Ends.
The unknown 5′ end of the partial primary clone was cloned by using a modified rapid amplification of cDNA ends (25) protocol and adapter ligated cDNA synthesized from the original tissue mRNA. The products obtained after two subsequent rounds of PCR by using adapter-specific and HOM-3.1.3 specific oligonucleotides (3.1.3-RCI 5′-TGG TCC CAA GAC AAG GAG GCA CCC AGC-3′, 3.1.3-RC2 5′-ATG TTT GCA GAT TGA GCT ACA GAG AAC-3′) were separated on agarose gels. DNA bands were excised and cloned into the TA cloning vector (Invitrogen).
Northern Blot Analysis.
Northern blot analyses were performed with RNA extracted from tumors and normal tissues using guanidinium thiocyanate (26). RNA integrity was checked by electrophoresis in formalin/4-morpholinepropanesulfonic acid gels. Gels containing 10 μg of RNA per lane were blotted onto nylon membranes (Hybond N, Amersham). After prehybridization, the membranes were incubated with the specific CA XII cDNA probe overnight at 65°C in Express Hyb solution (CLONTECH). A nick-translated 32P-labeled 754-bp fragment amplified from the 5′ end (sense 5′-CGC GAA GAT GCC CCG GCG CAG CCT-3′; antisense 5′-CTG CTC CTG GGA AAT TTC CAC GGG GTT-3′) was used as a probe. The membranes were then washed at progressively higher stringency, with the final wash in 1× standard saline citrate (SSC; 1[times SSC = 0.15 M sodium chloride/0.015 M sodium citrate, pH 7) and 0.2% SDS at 65°C. Autoradiography was conducted at −70°C using Kodak X-Omat-AR film and intensifying screen. The size of the full-length mRNA was approximated by comparison with the mobilities of 28S and 16S ribosomal RNA. After exposure the filters were stripped and rehybridized with a glyceraldehyde-3-phosphate dehydrogenase probe.
Reverse Transcription–PCR.
Total cellular RNA from a panel of normal tissues was primed with a (dT)18 oligonucleotide and reverse transcribed with Superscript RT (GIBCO). The CA XII transcript was amplified by using sequence-specific primers comprising an 806-bp 3′ fragment (sense 5′-GGA CAA ATG GGG ACA GGA AGG ATC AAG-3′; antisense 5′-GAG GAC ATT TCA TGC TGA CAA AAT GAG-3′).
Cloning of CA XII from Normal Kidney Tissues.
First strand cDNA was synthesized from 10 μg total RNA from normal kidney tissue by using a (dT)18 oligonucleotide and Superscript reverse transcriptase (GIBCO). Amplification of CA XII cDNA was performed by using the transcript-specific oligonucleotides comprising the entire ORF and a large part of the 3′ untranslated region (sense 5′-CGC GAA GAT GCC CCG GCG CAG CCT-3′; antisense 5′-GAG GAC ATT TCA TGC TGA CAA AAT GAG-3′). PCR was performed for 35 cycles with Taq polymerase (Goldstar, Eurogentec, Brussels) with an annealing temperature of 68°C. The resulting 2,492-bp product was cloned into TA cloning vector (Invitrogen) and was sequenced using plasmid- and transcript-specific oligonucleotides. The sequence was aligned and compared with the tumor-derived full-length transcript using dnasis software.
Screening of Allogenic Sera on Identified Specific Phage Clones.
Phage assay with allogenic sera has been described (15). Positive phages found to be reactive with the patient's serum were plaque purified, mixed with nonrecombinant phages as an internal negative control, and tested with allogenic sera from other patients and healthy controls extensively preabsorbed against E. coli antibodies.
Fluorescence in Situ Hybridization.
Metaphase spreads were prepared from peripheral blood of normal healthy donors by standard procedures (27). A fragment representing full-length CA XII was labeled by nick translation with Bio-16-dUTP according to the manufacturer's instructions (BRL). Labeled DNA (2 ng) was precipitated together with 20 μg of human placenta DNA as competitor and dissolved in 5 μl of hybridization buffer (50% deionized formamide/2× SSC/50 mM sodium phosphate, pH 7.0/10% dextran sulfate). Hybridization conditions and detection were as described (28).
Production of Histidine-Tagged CA XII for Immunization and Production of Polyclonal Rabbit Antiserum.
5′ truncated CA XII cDNA was amplified with proofreading Pfu DNA polymerase (Stratagene) by using RNA from patient's RCC and oligonucleotides from each end of the ORF (sense 5′-GGT TCC AAG TGG ACT TAT TTT GGT CCT-3′; antisense 5′-GGG ACC TCA AGC GTG GGC CTC AGT CTC-3′). The product was gel purified and ligated in frame to SmaI-digested, dephosphorylated and gel-purified pQE32 vector (Qiagen, Chatsworth, CA) to produce a fusion protein bearing an N-terminal 6-histidine tag. The construct was transformed into E. coli strain SG13009 (pREP4) and selected on kanamycin/ampicillin-containing plates. Individual colonies were picked and the fusion protein was expressed by induction with 2 mM isopropyl thiogalactoside and purified over Ni-nitrilotriacetate columns. A clone expressing a protein of the expected length was used for large-scale production after sequence verification. Cells were harvested 5 hr after induction, lysed in buffer A (8 M urea/100 mM Na2PO4/10 mM Tris⋅HCl, pH 8.0/0.01% Triton X-100) overnight. Debris was spun down and supernatant was loaded onto preequilibrated Ni-nitrilotriacetate resin, washed with 2 vol of buffer A (pH 8.0) and 10 vol of buffer A (pH 6.3), and eluted with 250 mM imidazole in buffer A. Polyclonal rabbit antisera were obtained from a custom antibody service (Eurogentec) after four immunizations with 100 μg of purified histidine-tagged fusion protein. Sera before and after immunization were tested by Western blot analysis for reactivity with the fusion protein.
Expression of CA XII cDNA in COS Cells.
The 1,292-bp 5′ fragment produced by HindIII, R1 digestion of a full-length clone was subcloned into the eukaryotic expression vector pCXN at the blunt-ended XhoI cloning site (29). COS-7 cells were transfected by the DEAE dextran method (30). After 72 hr, cells were scraped from the 60-mm dishes, washed, sedimented, and sonicated (twice for 20 sec each) in 25 mM Tris⋅H2SO4, pH 7.5 + 1 mM each of benzamidine, o-phenanthroline, and phenylmethylsulfonyl fluoride. The crude extract (200 μg of protein) was analyzed for CA activity as described (31, 32) in the presence and absence of 1 μM acetazolamide. For Western blot analysis, 20 μg of cell extract protein was resolved by SDS/PAGE (33) and the expressed protein was identified with polyclonal antibody to CA XII as first antibody, peroxidase-conjugated goat anti-rabbit IgG (Sigma) as second antibody, and the enhanced chemiluminescence reagent (Amersham). For deglycosylation, an equivalent amount of extract protein (20 μg) was treated with 100 milliunits of endoglycosidase (PNGase F) before SDS/PAGE and Western blot analysis (34).
Isolation of a Partial cDNA Clone and Full-Length Cloning of Human CA XII.
We screened 1.8 × 106 recombinant phage from a λ cDNA expression library prepared from mRNA isolated from a clear cell type RCC by using autologous serum from the RCC patient. Seven clones representing five different transcripts were identified. When these clones were used to probe Northern blots, one clone (HOM-RCC-3.1.3) was found to hybridize with a 4.5-kb transcript that appeared to be overexpressed in the RCC compared with the surrounding normal tissue (15, 17). Sequence analysis of this 1,670-bp clone identified a short coding sequence without an obvious translation start site. The 5′ end of this partial transcript was cloned by the rapid amplification of cDNA ends technique (25) in a 1,032-bp fragment. Alignment and connection of the two clones yielded a cDNA encoding 7 bp of 5′ untranslated sequence preceding a classical initiation codon (35) followed by a 1,062-bp ORF and 1,584 bp of 3′ untranslated sequence.
Nucleotide Sequence, Deduced Amino Acid Sequence, and Alignment of CA XII with Other Human CAs.
The ORF predicts a polypeptide sequence of 354 amino acids with a predicted mass of 39,448 Da that has features of a type I membrane protein. Database searches showed overall homologies to conserved regions of human CAs and CA-RPs of 30 to 42%. In accordance with the convention of naming CAs and CA-RPs in the order of their discovery, we designated this protein CA XII. Fig. 1 presents the predicted amino acid sequence of CA XII aligned below the sequences of human CAs I–VII and IX. The N-terminal extension of 29 amino acids fulfills the criteria for a signal sequence preceding the predicted cleavage between Gly-1 and Ser-1 (36, 37). A 261-amino acid CA-homology domain follows that contains most of the amino acid residues conserved among the active CAs (1). These are indicated by the boxes. The numbering above the aligned sequences indicates the amino acid number of the residues in CA I. The CA XII sequence contains all three Zn-binding histidine residues (His-94, His-96, and His-119) that are present in the active sites of catalytically active CAs (1, 38). Note also that His-64 is conserved. His-64 has been shown to contribute to the efficiency of high-activity CAs by serving as a proton shuttle between the Zn-bound water molecule and surrounding buffer molecules (39–41). The extracellular domain contains two potential sites for asparagine glycosylation and four cysteine residues, two of which are near positions of two cysteines in CA IV and CA VI that form an important disulfide bond that stabilizes the structure of each of these two CAs (42). Hydropathy plots indicate a 26-amino acid hydrophobic segment (underlined in Fig. 1) that presumably serves as a transmembrane domain, that is followed by a 29-amino acid hydrophilic cytoplasmic C terminus that contains two potential sites for phosphorylation.
Expression of CA XII Immunoreactivity and Catalytic Activity in COS Cells.
To characterize the protein expressed from the CA XII cDNA, we subcloned the coding sequence into a eukaryotic expression vector and measured the immunoreactivity and catalytic activity of the expressed protein in extracts of transfected COS cells. Fig. 2 shows the Western blot of untreated and PNGase F-treated extracts. A 43- to 44-kDa doublet was identified in the CA XII transfected COS cells by the polyclonal antibody to CA XII that collapsed to a single band at ≈39 kDa after PNGase F treatment, consistent with removal of two oligosaccharide chains. Table 1 presents results of measurements of CA activity in the extracts of COS cells infected with vectors expressing CA XII, CA IV, and vector only. The extract of CA XII transfected cells had ≈16% the activity of the extract from cells transfected with bovine CA IV, which is one of the highest activity isozymes. Both CA XII and CA IV activities were sensitive to inhibition by acetazolamide. All of the immunoreactive CA XII sedimented with membranes when the extract was centrifuged at 50,000 × g for 30 min (unpublished observations). Although purification of CA XII will be required to compare its kinetic properties with those of other CAs, it is clear from the above results that expressed recombinant CA XII has appreciable catalytic activity.
Tissue Expression Studies by Reverse Transcription–PCR and Northern Analysis.
Reverse transcription–PCR with CA XII-specific primers detected this transcript in RNA from many normal tissues (lung, liver, colon, stomach, skeletal muscle, skin, kidney, spleen, tonsil, lymph node, peripheral blood lymphocytes, activated peripheral blood lymphocytes, bladder, breast, uterus, ovary, brain, prostate, and skin) as well as in a panel of different human neoplasms (RCC, colorectal cancer, lung cancer, and breast cancer). However, on Northern blots of RNA from normal tissues, the ≈4.5-kb transcript was detectable only in RNA from kidney, colon, and phorbol 12-myristate 13-acetate-activated peripheral blood lymphocytes (Fig. 3). Analysis of RNA from RCC tumor tissues demonstrated that CA XII is overexpressed (as compared with the corresponding normal renal tissue) in 10% of renal carcinomas of clear cell type as was the case in the tumor of the RCC patient from whose cDNA library the CA XII cDNA was cloned (15–17).
Chromosomal Localization.
Fluorescence in situ hybridization was performed to localize the gene for human CA XII. Several independent hybridizations revealed a strong signal on the long arm of chromosome 15, namely at 15q22 (Fig. 4). No hybridization signal was seen on chromosomes 1, 8, 16, or 17 where other CAs have been localized (1).
CA XII Is Not Mutated in RCC.
To exclude mutations of the tumor-derived CA XII transcript as a reason for immunogenicity in the autologous patient, we cloned the normal counterpart cDNA from normal human kidney by reverse transcription–PCR amplification of the entire transcript. Sequencing revealed no differences from the tumor-derived cDNA, ruling out mutational alterations as a mechanism for the initiation of the humoral immune response in the patient.
Incidence of Anti-CA XII Antibodies in Human Sera.
Other human sera were tested in the above described phage assay for antibodies to CA XII. Four of 30 RCC patients and 1 of 30 normal controls showed antibodies against the CA XII phage clone, whereas none of 11 astrocytoma patients and none of 17 patients suffering from Hodgkin's disease had any serum response.
Two other active CAs are membrane proteins like CA XII. CA IV, the first membrane isozyme described, is a glycosyl-phosphatidylinositol-anchored protein expressed on the apical surface of epithelial cells in colon (43), kidney (44), male genitourinary tract (45, 46), and on the plasma face of endothelial cells of several capillary beds, including the pulmonary microvasculature (47). CA IV facilitates the reversible hydration of CO2 at sites where CO2 and HCO3− flux across membranes needs to be very rapid. The second membrane CA described is CA IX, which, like CA XII, was discovered as a tumor-associated CA and has recently been found to be expressed in many RCCs of clear cell type (48). The CA domain is in the middle of the multidomain CA IX that has been reported to have transforming potential and to be overexpressed in tumors of tissues in which it is not normally expressed (10, 12, 48). N-terminal to the CA domain is a highly acidic (44%) proteoglycan-related domain (59 residues) that shows 37% sequence identity to the keratan sulfate attachment domain of the human cartilage aggregation proteoglycan, aggrecan (49). Whether the CA activity in CA IX is required for its transforming potential, or the CA domain might function in a ligand-binding role analogous to the acatalytic domain in RPTPβ and RPTPγ (7, 8, 50), remains to be established.
The evidence that CA XII is expressed in human cancers comes from several independent sources. While this work was in progress, we became aware of U.S. patent number 5,589,579, in which the inventors R. M. Torczynski and A. P. Bollon claim the DNA sequence and protein it encodes to be novel and specific for human lung cancer cells (51). The patent summarized Northern blot data showing expression in normal pancreas and kidney, and 50-fold more expression in the lung carcinoma cell line A549. The fact that overexpression of CA XII was seen in 10% of RCC of clear cell type provides further evidence that overexpression of CA XII is cancer-related, even if not limited to lung cancers. Although we also found low levels of expression detectable by PCR in many other tissues, the appropriate transcript on Northern blots was only seen in RNA from kidney, intestine, and phorbol myristate acetate-activated peripheral blood lymphocytes.
After this work was completed, we learned of a nearly identical sequence entered in GenBank by S. V. Ivanov, I. Kuzmin, M. H. Wei, S. Pack, L. Geil, E. Stanbridge, and M.I. Lerman entitled "A new family member, CA 12, of α-carbonic anhydrases is downregulated by the VHL gene" (GenBank accession no. AF037335). This sequence contains 108 additional base pairs of 5′ untranslated region. Regulation of CA XII expression by the VHL tumor suppressor gene could explain why we found CA XII overexpressed in RCCs, because inactivation of both copies of the VHL gene is reported to be an early event in development of clear-cell carcinomas (52).
The CA XII cDNA reported here was detected by an IgG antibody in the serum of the cancer patient (15–17). Serum antibodies to CA I and CA II, both rather widely expressed cytoplasmic CAs, have been described in humans with different autoimmune diseases like Sjögren syndrome, immune cholangiopathies, scleroderma, and systemic lupus erythematosus (20, 53–56). However, serum antibodies to CAs in tumor patients have not been investigated. In preliminary studies, we found 4 of 30 RCC patients to have high-titer antibodies against CA XII. As has been reported for autoantibodies to other CAs (54, 56, 57), we also detected antibodies to CA XII in 1 of 30 apparently healthy controls tested. The significance of this finding is not yet clear.
Antibodies to a protein in cancer patients might result from a response to a new epitope created by mutational alteration of the protein, such as in mutant p53 or mutant ras (58, 59). However, we established by sequencing that the CA XII cDNA in the RCC of our patient was identical to the cDNA derived from normal kidney. Thus, other mechanisms must be operative in this patient. It is known that overexpression itself may initiate immune responses to structurally normal proteins, as has been reported for HER-2/neu (60). Possibly, overexpression of the CA XII protein in the RCC of our patients led to the immune responses. An important question to be resolved by extending these studies is whether the autoantibodies to CA XII will be useful markers in screening (or following progression in) patients with RCC and other cancers.
The finding of a second membrane CA associated with human cancer raises another interesting question: Is overexpression of these membrane CAs simply a byproduct of malignant transformation, or do the membrane CAs actually contribute to malignant transformation and/or tumor progression? Conceivably, CA IX and CA XII (both of which are expressed in RCCs) could contribute a ligand-binding domain that is involved in transformation. Alternatively, the transformation might be enhanced by the presence of CA activity at the membrane of highly proliferating cells. If the CA activity at the membrane itself played a role in transformation, it would raise the possibility that isozyme-specific CA inhibitors might be developed that would diminish or abrogate the transforming potential of the membrane CAs.
We thank Dr. Lloyd Old for continuing motivating discussion. This work was supported by the Deutsche Forschungsgemeinschaft and by National Institutes of Health Grant DK40163.
- renal cell cancer;
- carbonic anhydrase;
- carbonic anhydrase-related protein;
- receptor protein tyrosine kinase phosphatase;
- PNGase,
- endoglycosidase
- Accepted April 24, 1998.
- Copyright © 1998, The National Academy of Sciences
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Carole Teresa Macrina
Carole passed away in October 2018.
Carole was a resident of Gaithersburg, Maryland at the time of passing.
Carole Teresa Macrina Viewing: Thursday November 1, 2018 from 6:00 PM to 8:00 PM Friday November 2, 2018 at 10:30 AM St. Martin of Tours Catholic Church, Gaithersburg, MD On Monday October 29, 2018, Carole T. Macrina of Gaithersburg, MD. Mass of Christian Burial will be offered at St. Martin of Tours Catholic Church, 201 S. Frederick Ave., Gaithersburg, MD 20877 on Friday November 2, 2018 at 10:30 AM. Interment: Private.
In lieu of flowers contributions may be made in her name to ASIA Families, 18536 Perdido Bay Terr., Leesburg, VA 20176 or online at: www.asiafamilies.org.


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Svetoslav was absolutely excellent, he did everything asked of him & was incredibly helpful, patient (even when the original brief was added to) & was a pleasure to work with. An outstanding programmer & very professional. I look forward to working with Svetoslav on future projects & already have work in additional work that I aim to send him. Thank you Svetoslav A++++++
Super-amazing awesome coder-extreme! Hire Svetoslav today with confidence.
Super-amazing awesome! What a genius, his product is better than you can imagine!
Thank you Svetoslav, your knowledge of MODX was invaluable. I'm sure we'll work together again soon. Thanks
I received and tediously compared 12 different proposals, several of them excellent, before I selected Svetoslav. My diligence was rewarded with his excellent results. This was far from a trivial task, though Svetoslav's elegant solution made it seem so.
A somewhat experienced programmer myself, I only turned to PPH after realizing I did not have the background to solve this problem. After having the job turned back by two other "Opencart Experts" who realized they, too, were unable to accomplish what I failed, I posted this job and am glad I did. Svetoslav's resume clearly showed incredible knowledge and experience, and his results on this job simply validated that. Bravo!
In a word, excellent. Great communication and hard work to get the job done. Thanks!
Fantastic to work with, and completed in record time.
Svetoslav V was great, communicated very well and the work was done ahead of schedule can highly recommend and will use again.