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Latest findings have shown that embryonic vascular progenitor cells are able of differentiating into mural and endothelial cells. clashes with angiogenesis, the term used to the post-natal neovascularization that outcomes from the growth and redesigning of differentiated endothelial cells from preexisting capillary vessels (Risau, 1997). Lately, embryonic control cell (ESC)Cderived vascular progenitor cells possess been proven to differentiate into both mural Esomeprazole Magnesium trihydrate and endothelial cells (Yamashita et al., 2000; Marchetti et al., 2002; Ema et al., 2003). Furthermore, bone fragments marrowCderived vascular progenitor cells moving in adult peripheral bloodstream have got been proven to consist of progenitor cells offering rise to both types of cells (Asahara et al., 1997; Sata et al., 2002), and to contribute to angiogenesis. Endothelial cell tyrosine kinase receptors for VEGFs possess been suggested as a factor in the control of difference, Esomeprazole Magnesium trihydrate development, and condition of endothelial cells. VEGF stimulates endothelial difference of vascular progenitor cells made from ESC (Yamashita et al., 2000) and adult bone fragments marrow (Asahara et al., 1997). VEGF regulates development of differentiated endothelial cells also, and boosts vascular permeability (Senger et al., 1983). Although these properties of VEGF are of great importance in the procedures of angiogenesis and vasculogenesis, VEGF may lead to tissues edema because angiogenesis is certainly followed by an boost in vascular permeability implemented by yacht sprouting. VEGF acts in endothelial cells exclusively; nevertheless, relationship between endothelial cells and mural cells (pericytes and vascular simple muscles cells) is certainly important for advancement of vascular tissue and maintenance of their homeostasis in both embryonic and adult tissue (Folkman and D'Amore, 1996). TGF- superfamily protein have got been suggested as a factor as one of cytokines that provide such relationship (Carmeliet, 2000). Associates of the TGF- superfamily indication via heteromeric processes of type type and II We serine/threonine kinase receptors. Upon ligand holding, the constitutively energetic type II receptor kinase phosphorylates the type I receptor which, in convert, activates the downstream indication transduction cascades, including Smad paths. Activins and TGF-s join to type I receptors known as activin receptor-like kinase (ALK)-4 and -5, respectively. The turned on type I receptors phosphorylate receptor-regulated Smad (R-Smad) meats. Smad2 and 3 transduce indicators for activins and TGF-s, whereas ZNF143 Smad1, 5, and 8 are particular for signaling of bone fragments morphogenetic protein (BMPs; Massague, 1998). As an exemption, ALK-1, portrayed in endothelial cells particularly, binds TGF- and activates Esomeprazole Magnesium trihydrate Smad1/5 paths (Oh et al., 2000). The jobs of the TGF- superfamily in vasculogenesis possess been recommended by the results that knockout rodents lacking in several TGF- superfamily signaling elements display flaws in vascular tissue (Goumans and Mummery, 2000). Furthermore, heterozygous mutations in individual genetics code for endoglin, an accessories proteins for the TGF- receptor complicated, and/or ALK-1 trigger hereditary hemorrhagic telangiectasia (Marchuk, 1998). Nevertheless, the absence of in vitro systems consisting of natural populations of these mobile lineages provides hampered dissection of the jobs of TGF- superfamily signaling in vascular advancement. To elucidate the jobs of TGF- superfamily signaling in vascular difference from their progenitors, we Esomeprazole Magnesium trihydrate utilized in vitro difference systems made from mouse ESC and 8.5Ctimes postcoitum (dpc) embryos. We present that TGF- and inhibit growth and piece formation of ESC-derived endothelial cells activin. The results of TGF- on endothelial condition are at least partially mediated by its down-regulation of the phrase of claudin-5, an endothelial particular component of restricted junctions (TJs). Strangely enough, SB-431542, a artificial molecule that prevents the kinases of receptors for activin and TGF-, caused sheet and growth formation of Esomeprazole Magnesium trihydrate ESC-derived endothelial cells. Finally, we present that TGF- down-regulated the development and condition of endothelial cells made from 8.5-dpc embryos, whereas SB-431542 exhibited the contrary effects. These total results suggest that endogenous TGF-/activin alerts play essential roles in regulating vascular growth and permeability. Outcomes TGF- and activin hinder development of ESC-derived endothelial bed linens Regarding to a prior survey (Yamashita et al., 2000), undifferentiated mouse ESCs had been cultured for 3 n on collagen IVCcoated meals with 10% FCS to induce Flk1+ cells, which had been filtered by cell selecting using anti-Flk1 antibody. After an extra 3 n of lifestyle of Flk1+ cells with 10% FCS, we attained cells positive for a mural cell gun, -simple muscles actin (SMA; Fig. 1 a). When 30 ng/ml VEGF was added, we had been capable to get platelet-endothelial cell adhesion molecule 1 (PECAM1)Cpositive bed linens of endothelial cells. The staying cells encircling the bed linens had been positive.
Author Archives: Leroy Austin
The hepatopulmonary syndrome (HPS) develops when pulmonary vasodilatation potential clients to
The hepatopulmonary syndrome (HPS) develops when pulmonary vasodilatation potential clients to abnormal gas exchange. pets that had been not really noticed in control versions assisting a picky decrease of alveolar airspace. Furthermore, that administration was discovered by us of TNF-, the bile acidity, chenodeoxycholic acidity, and FXR nuclear receptor service (GW4064) caused apoptosis and reduced SP-B and SP-C creation in alveolar epithelial cells check or evaluation of difference with Bonferroni modification for multiple evaluations between organizations. Statistical significance was specified as scam) suggesting decreased lung surfactant proteins creation. To assess whether identical occasions happen in the lung after PVL, we likened Arctiin manufacture two typical surfactant aminoacids, SP-A and pro-SP-C proteins amounts in 3 wk CBDL and 3 wk PVL pets (Shape 2A). In Arctiin manufacture comparison to GATA6 3 wk CBDL lung area where surfactant proteins amounts had been reduced, the phrase of SP-A and pro-SP-C amounts continued to be unrevised in 3 wk PVL pets. To define if adjustments in AT2 cell surfactant creation are component of a general impact, we tested lung AQP5 (a particular AT1 cell gun) amounts. We noticed no significant change in AQP5 proteins amounts after CBDL (Shape 1), assisting a exclusive impact on AT2 cells. Shape 1 Pulmonary surfactant connected proteins (SP) amounts after CBDL. Shape 2 Assessment of pulmonary connected proteins (SP) amounts and morphologic adjustments in CBDL and PVL pets. Pulmonary Morphologic Adjustments after CBDL and PVL To assess whether the decrease in surfactant aminoacids after CBDL can be connected with morphologic changes in the lung, we tested lung quantities using a drinking water displacement technique and quantified the typical alveolar size indicated by mean chord size (Lm) in control and CBDL pets (Shape 2B). Relatives to PVL and scam pets, total correct lung quantity (0.87 fold vs control, p<0.05) and mean chord size (25.90.9 vs 38.41.8 m, p<0.05) decreased significantly after CBDL indicating a selective decrease of alveolar airspace. These results display that decreased pulmonary surfactant proteins amounts after CBDL are followed by a problem in maintenance of alveolar sincerity causing in alveolar failure and a decrease in lung quantity. Pulmonary SP-C Localization and Phrase after CBDL Among the surfactant aminoacids, SP-C phrase can be limited to AT2 cells and it can be the most hydrophobic therefore backing the alveolar surface area in mammalian lung. As a measure of AT2 cell sincerity, we examined SP-C mRNA amounts and immunohistochemical localization and measured the amounts of AT2 cells (SP-C positive) (Shape 3). Relatives to control pets, SP-C mRNA amounts started decreasing within 1 week after CBDL, and decreased over 2 to 3 weeks progressively. Likewise, in control pets, AT2 cells with shiny and very clear SP-C discoloration were distributed at the edges of alveoli traditionally. In 3 week CBDL pets, the numbers of SP-C positive cells were reduced in the lung supporting a reduction in AT2 cells significantly. Shape 3 Alveolar type II epithelial cell (AT2) amounts and SP-C mRNA amounts after CBDL. Arctiin manufacture Pulmonary AT2 Cell Destiny after CBDL To define what accounts for the lower in SP-C activity and reduction of SP-C positive cells in lung after CBDL, we evaluated apoptosis by Arctiin manufacture TUNEL yellowing and cleaved caspase-3 proteins amounts (Shape 4A and 4C) and localised apoptotic cells by double-immunoflourescence yellowing with TUNEL and SP-C (Shape 4B). Relatives to control, there was a significant boost in TUNEL positive cells and cleaved caspase-3 proteins phrase in lung after CBDL. The majority of apoptotic cells co-stained for SP-C and TUNEL indicating that they were AT2 cells. To assess AT2 cell apoptosis in response to CBDL straight, we isolated lung AT2 cells from CBDL and control animals and measured proteins levels of cleaved capase-3. The chastity of the cells was >90% as established by SP-C IF yellowing (Shape S i90001), constant with earlier research [40]C[43]. There was a significant boost in cleaved caspase-3 creation in AT2 cells separated from CBDL lung, credit reporting our findings. Shape 4 Pulmonary AT2 cell apoptosis after Arctiin manufacture CBDL. Modulation of AT2 Cell Surfactant Proteins Phrase and Apoptosis results displaying improved amounts of TUNEL positive AT2 cells and improved cleaved caspase-3 amounts in lung and separated AT2 cells from CBDL confirm AT2 cell apoptosis. Our results that bile acids (CDCA), a particular bile acidity nuclear receptor FXR TNF- and agonist, each caused both cell apoptosis.
MVA is an attenuated stress of vaccinia computer virus (VACV) that
MVA is an attenuated stress of vaccinia computer virus (VACV) that is a popular vaccine vector. the EGFR. Here it was observed that NF-B was activated Rabbit polyclonal to Cannabinoid R2 in 293T cells transfected with a plasmid encoding the C11R gene. Silencing by small interfering RNA (siRNA) or deletion of the C11R gene (MVAC11R) reduced both MVA-induced ERK2 and NF-B activation in 293T cells or the keratinocyte GDC-0980 line Hacat, suggesting that this mechanism of MVA-induced NF-B activation may be common for several cell types. INTRODUCTION MVA is usually an attenuated vaccinia computer virus (VACV) that was created by serially passaging wild-type VACV in chicken embryo fibroblasts (CEFs) >500 occasions (34). MVA was used safely as a smallpox vaccine in the 1970s (25) GDC-0980 and is usually more advantageous than wild-type VACV because GDC-0980 it is usually replication incompetent in all human cells tested (3, 8). However, a drawback for its use as a smallpox vaccine is usually that higher or multiple doses of MVA are required to achieve the anti-VACV antibody response obtained with a single dose of wild-type VACV (55). More recently, MVA has been used as a vector for vaccines against a range of contagious illnesses of human beings (Helps, malaria) and creatures (rabies) (26). Wild-type VACV traces, such as WR, encode immunoevasion genetics, whose items hinder NF-B, whereas these genetics are lacking from MVA (2, 38). As a total result, WR infections prevents NF-B, whereas MVA infections activates NF-B (41). While these data would recommend that NF-B account activation reduces the immunogenicity of MVA, one cannot respect this model as established, because other profound distinctions exist between MVA and WR. In evaluation to WR, MVA is certainly duplication inexperienced (8), and MVA does not have numerous immunoevasion genetics whose items dampen resistant replies various other than NF-B account activation (2). To even more assess the romantic relationship between NF-B and the immunogenicity of MVA accurately, our reason is certainly to recognize the virus-like component(t) accountable for MVA-induced NF-B account activation and to make use of this details to make MVA constructs in which NF-B-activating capability is certainly affected. Some of these elements are known already. For example, in GDC-0980 293T individual fibroblasts, MVA-induced NF-B account activation needs the phrase of the early course of poxviral genetics (33). Next, an early gene item(s i9000) possibly straight or not directly stimulates account activation of the MEK1 mitogen-activated proteins kinase (MAPK), causing in the account activation of extracellular signal-related kinase 2 (ERK2), an event that is certainly upstream of and required for MVA-induced NF-B account activation (18, 33). The skin development aspect receptor (EGFR), when communicating with its cognate EGF, sparks receptor account activation to induce events, including MEK/ERK activation, proliferation, cell survival, and NF-B activation (44). The EGFR stimulates these myriad events via unique upstream signal transduction events. For example, EGFR-Sos-Ras-Raf-MEK1 activation activates the ERK1 and ERK2 (ERK1/2) proteins, GDC-0980 producing in cellular proliferation (44). EGFR autophosphorylation can also stimulate NF-B-inducing kinase (NIK) (23), and it has been reported that EGFR-induced NIK activation results in NF-B activation (23). The goal of this study was to identify a viral protein(h) that is usually an upstream activator of this ERK2CNF-B activation pathway, using 293T cells as a model system. The data show that the EGFR is usually partially responsible for initiating this pathway during MVA contamination. While the EGFR classically is usually known to activate ERK2 via the Sos-Ras-Raf-MEK1 pathway, we show here that a Ras- and Raf-independent pathway causes EGFR-induced NF-B activation (44). MVA, like all other orthopoxviruses, encodes vaccinia computer virus growth factor (VGF), a homolog to EGF (49). We observed that either silencing or removal of the gene encoding VGF (C11R) resulted in an MVA computer virus with reduced ability to activate ERK2 and NF-B. This phenotype was observed during contamination of 293T cells and.
Purpose of the scholarly research To explore the mechanism of oxidative
Purpose of the scholarly research To explore the mechanism of oxidative tension in the advancement of prostate cancers, right here we compared 4-hydroxynonenal (4-HNE)- treated LNCaP (hormone-sensitive) and DU145 (hormone insensitive) cells with significant differences in awareness to androgen. induction of MK-2866 apoptosis can end up being inhibited by overexpression of GSTA-4. A conclusion These research recommend that 4-HNE promotes prostate cancers cell apoptosis through the g53 signaling pathway; the variations of level of sensitivity to 4-HNE in LNCaP and DU145 cells may become related to the androgen level of sensitivity of prostate malignancy cells; and the 4-HNE-induced p53-mediated apoptosis transmission is definitely controlled by GSTA-4. test. Ideals of p < 0.05 were considered to be statistically significant. Results 4-Hydroxynonenal causes apoptosis in DU-145 and LNCaP cells The effectiveness of 4-HNE in inhibiting cell growth was assessed by MTT analysis. The results reported in Table 1 indicated that significant variations were exposed between the two models of prostate malignancy utilized (DU145 and LNCaP). In particular, HNE inhibited MK-2866 the growth of LNCaP cells, starting at 20 M for 24 h, respectively, and there was dose- and time-dependent inhibition of proliferation by HNE. A significant decrease in the viable cell population, i.e. 70.45%, was observed in cells treated with 100 M of 4-HNE on LNCaP for 48 h. In contrast, in DU145 cells, HNE caused only a slight reduction in cell proliferation starting at 40 M for 24 h without statistical significance; even if exposed to 100 M HNE (P > 0.05), DU145 manifested a relative resistance to supra-physiological concentrations of 4-HNE toxicity. 4-Hydroxynonenal-induced apoptosis in DU145 and LNCaP cells was further analyzed by flow cytometry. As the results in Figure 1 show, after treatment of LNCaP cells with different concentrations of 4-HNE ranging from 20 to 80 M for 24 h, the late apoptotic or necrotic cells increased from 16.5% to 48.2% in LNCaP cells, and from 17.2% to 22.8% in DU145 cells in a dose-dependent manner, indicating that DU145 cells are more resistant to 4-HNE toxicity compared to the more susceptible LNCaP cells. Fig. 1 Effects of 4-HNE on DU145 and LNCaP cell apoptosis analyzed by flow cytometry with Annexin V-FITC, PI staining, and TUNEL assay. Annexin V-FITC in conjunction with PI staining was used to distinguish early apoptotic from late apoptotic or necrotic cells. … Table 1 Inhibitory effect of 4-HNE on the cell proliferation of DU145 and LNCaP cells 4-Hydroxynonenal activates apoptotic signaling in LNCaP cells Numerous studies have shown that 4-HNE in many different cell types has the effect of inducing cell apoptosis [19C25]. P53 is an important gene involved in internal cell apoptosis signaling pathways. Based on these total results, 4-HNE concentrations of 0C40 Meters had been utilized to examine its impact on apoptotic signaling in LNCaP and DU145 cells. Outcomes MK-2866 shown in Shape 2A demonstrated that within the 0C40 Meters range, 4-HNE triggered a dose-dependent boost in p-p53, g21, Caspase-3 and Bax in LNCaP related g53 proteins which was not really transformed in LNCaP cells, but an impact of Tmem1 4-HNE-induced service of p53, p-p53, p21, Bax and caspase-3 was not observed in DU-145 cells treated with 4-HNE (Fig. 2B). These results showed that activation of p53-mediated intrinsic apoptotic signaling occurred in LNCaP rather than DU145 cells when uncovered to 4-HNE, exhibiting significant differences between DU145 and LNCaP cells. Further studies are needed to evaluate the difference between LNCaP and DU145 cells s in 4-HNE-induced apoptosis in the p53-mediated pathway. Fig. 2 Effect of 4-HNE on p53-mediated intrinsic apoptotic pathway in LNCaP (A) and DU145 (W). DU145 and LNCaP cells were treated with different concentrations of 4-HNE (0C40 M) for 24 h at 37C, respectively. Total protein lysates were … 4-Hydroxynonenal activates JNK signaling pathway in LNCaP and DU-145 cells A rise in intracellular levels of 4-HNE is usually a common incidence when cells are open to stressors such as oxidant chemical substances, UV light and high temperature surprise, and suffered account activation of JNK takes place during stress-induced apoptosis in many different cell types [19, 25C27]. The JNK activation is through its phosphorylation on Thr and Ser residues within its N and C-terminal regions. As a result, we examined the phosphorylation status of JNK in 4-HNE-treated DU145 and LNCaP cells using the anti-phospho-antibody which specifically detects phosphorylated JNK. As shown in Physique 3B, 4-HNE caused a dose-dependent increase in the phosphorylation of JNK in DU145 cells, but JNK activation (phosphorylation) was not observed in.
Introduction Extreme respiratory distress syndrome (ARDS) is the most common cause
Introduction Extreme respiratory distress syndrome (ARDS) is the most common cause of respiratory failure among critically ill subjects, sepsis and severe bacterial pneumonia being the most common causes. ARDS. non-e of the pets treated with saline option retrieved to regular base beliefs during the 6?hours that the pets were implemented. In comparison, lamb treated with a dosage of 40 million adult control cells came back their amounts of air in their bloodstream to base two hours after the cells had been infused. Likewise, improvements in co2 dioxide (Company2) measurement, pulmonary vascular stresses and irritation were observed and confirmed by histology and by the decrease in lung edema. Conclusions We came to the conclusion that instillation of adult non-hematopoietic stem cells Rabbit Polyclonal to GNA14 can diminish the impact of endotoxin and accelerate recovery of oxygenation, CO2 removal and inflammation in the ovine model, making the use of adult stem cells a real option for future therapies for ARDS. Introduction Acute respiratory distress syndrome (ARDS) is usually a common clinical entity and a major cause of morbidity and mortality in the crucial care setting [1]. The recent Berlin definition classifies it in three different degrees of severity according to the level of hypoxemia, calculated as: moderate300 to 201?mmHg partial pressure of oxygen (PaO2)/fraction of inspired oxygen (FiO2); moderate, 200 to 101?mmHg PaO2/FIO2; and severe, PaO2/FIO2??100?mmHg [2,3]. Although ARDS results from a wide variety of disorders, sepsis is usually its main cause and the risk factor most associated with high mortality [4-6]. Regardless of the cause, the alveolar epithelium and capillary endothelium are affected, leading to an increase in permeability allowing protein-rich fluid to accumulate in the alveolar space [7-9]. The loss of epithelial honesty disrupts alveolar clearance and production of surfactant [10-12]. In addition to the alveolar damage, there is an influx of circulating inflammatory formation and cells of hyaline membranes generally caused by the mechanical ventilation. If the inflammatory procedure is certainly serious more than enough, there will end up being resulting disorganized fix causing in fibrosis [13]. Existing therapy is certainly presently limited to supporting treatment [14,15]. A novel potential therapy for ARDS is usually the use of bone marrow-derived mesenchymal stem cells (B-MSC) [16-20]. We have previously exhibited that the infusion of B-MSC isolated from mice and swine prevented inflammation and aberrant repair of endotoxin-induced lung injury in both species [19,21-23]. These effects, together with the recovery of Pazopanib liquid measurement and the reduce in microbial development, have got also been proven in an perfused individual lung model of septic ARDS [16]. There is certainly solid proof that in versions of ARDS, after infusion B-MSC are turned on causing release of multiple soluble elements that outcomes in considerably lower amounts of inflammatory cytokines in both plasma and bronchoalveolar lavage (BAL) [19,20,24,25]. B-MSC are also capable to alter the systemic redox environment quality of ARDS to a much less oxidizing worth [18,26-30] and restore the alveolar endothelium and epithelium condition and permeability, lowering airspace neutrophils [30,31]. The transfer of useful mitochondria from B-MSC to the epithelium provides been established instrumental in the fix procedure of the lung [32-34] and B-MSCs possess also been proven to possess anti-bacterial results that are extremely helpful in Pazopanib the septic environment [35-38]. We designed a preclinical huge pet model of endotoxin-induced ARDS in purchase to assess the basic safety and efficiency of the make use of of adult bone fragments marrow-derived control cells, called MultiStem (Athersys, Cleveland, Oh yeah) in the treatment of moderate-to-severe ARDS. In the present research, lamb with lipopolysaccharide (LPS)-activated ARDS received Great Production Practice (GMP)-MultiStem, which possess been utilized in scientific studies for areas various Pazopanib other than the lung [39,40], with no toxicity reported. Our outcomes recommend that MultiStem possess the capability to decrease the intensity and duration of the damage, without discovered supplementary dangerous results. This enables us to propose the translation of bone fragments marrow-derived control cells into scientific research for the treatment of sufferers with ARDS. Strategies Pet model Fourteen adult Dorsett Get across lamb considering 36.5 to 65?kg were used in the present study. All animals received humane care in compliance with the Principles of Laboratory Animal Care formulated by the National Society for Medical Research and the Guideline for the Care and Use of Laboratory Animals prepared by the Institute of Laboratory Animal Resources and published by the National Institutes of Health (NIH) (NIH no. 86C23). The Institutional Animal Care and Use Committee (IACUC) for Animal Research of the University or college of Pittsburgh approved all experimental procedures in advance. The Pazopanib use of human stem cells in animals was approved by the Human Stem Cell Research Oversight (hSCRO) Office at the University or college of Pittsburgh. A Swan-Ganz catheter was inserted through the jugular vein, after which open chest superior vena cava.
Hereditary studies have recognized common alternatives within the intergenic region (and
Hereditary studies have recognized common alternatives within the intergenic region (and myeloblastosis oncogene about chromosome 6q that are connected with elevated fetal hemoglobin (HbF) levels and alterations of additional clinically important human being erythroid traits. regularly used for the analysis and monitoring of a wide range of disorders as well as overall human being health. Significant variant in these guidelines, which is highly heritable, happens among humans (1, 2). Genome-wide association studies (GWAS) and additional studies possess looked into the genetic basis of variant in erythroid and additional hematological characteristics within different ethnic populations. As observed in the majority of association studies, some genome-wide, sequence variations modulating human being characteristics are mainly located in noncoding areas of the genome (3), complicating the practical model of their effects. A arranged of common intergenic SNPs at chromosome 6q23 offers been consistently recognized as highly connected with clinically important human being erythroid characteristics (4C13) (Table ?(Table1).1). Prominent among these characteristics is definitely the perseverance of fetal hemoglobin (Hb) in adults (HbF, assessed as %HbF of total Hb or as proportion of reddish blood cells transporting HbF [%N cells] (4, 14, 15)). General diagnostic erythroid guidelines such as RBC, MCV, MCH, and others (5, 7, 8, 10, 13) have also been found to become highly connected with the Bergenin (Cuscutin) presence of the 6q23 variations. Characteristics with weaker, but significant association are packed blood cell volume (PCV, also referred to as hematocrit) (7, 10, 13), total Hb (13), HbA2 (12), and actually nonerythroid characteristics (i.at the., monocyte and platelet counts) (5, 10). The genetic rules of HbF levels is definitely of particular restorative interest, as improved HbF levels significantly ameliorate disease severity of the 2 main -hemoglobinopathies -thalassemias and sickle cell disease (16, 17) which symbolize some of the most common human being genetic disorders (18). Erythroid-trait connected SNPs (Table ?(Table1)1) reside within a 126-kb intergenic region between the and genes (Number ?(Figure1A).1A). As originally reported in studies looking into the genetic basis of variant in HbF levels (4, 15), a small quantity of these SNPs were Bergenin (Cuscutin) demonstrated to display an especially strong association; these observations were mainly confirmed for the additional erythroid phenotypes looked into (7, 8, 10, 13). These SNPs are closely linked with each additional and span a region of about 24 kb (originally termed intergenic polymorphism block 2 [HMIP-2]) (4, 7, 11). Association of these HMIP-2 SNPs with the erythroid characteristics offers been replicated and validated in populations from varied ethnic experience (6C8, 10). Despite considerable genetic evidence, a obvious mechanistic basis for the association between the intergenic SNPs and erythroid biology offers remained evasive, although the 2 flanking genes (and intergenic region connected with HbF levels and additional human being erythroid characteristics. Table 1 Human being erythroid phenotypes connected with intergenic variations Whereas the function of in reddish blood cell development is definitely uncharacterized, the gene (encoding the c-MYB transcription element [TF]) is definitely a important regulator of hematopoiesis and erythropoiesis (23, 24). c-MYB takes on an essential part in controlling the erythroid cellular expansion/differentiation balance (25) and manages Bergenin (Cuscutin) HbF levels through an undefined mechanism (19, 20). The practical importance of the intergenic region was 1st observed when transgene attachment within the murine intergenic region almost completely abolished transcription and resulted in severe anemia (22). A recently reported follow-up investigation mapped the location of transgene attachment to the HMIP-2 orthologous region and showed elevated levels Goat polyclonal to IgG (H+L)(PE) of embryonic globin genes in splenic erythroid cells of these transgenic mice (21), confirming the importance of the intergenic region for globin gene rules in the mouse. We previously recognized several distal regulatory elements in the mouse intergenic region that regulate transcription by literally interacting with the promoter and 1st intron in erythroid progenitors via chromatin looping (26,.
Objective Chronic pancreatitis is usually a modern inflammatory disorder of the
Objective Chronic pancreatitis is usually a modern inflammatory disorder of the pancreas characterized by long lasting destruction of acinar cells. some cases loss of catalytic activity also; as a result, we suggested that elevated risk for persistent pancreatitis in mutation companies is certainly greatest described by the decreased trypsin degrading activity within the pancreas.[16] The trypsin-dependent disease super model tiffany livingston described above assumes that gain or reduction of BMS 378806 catalytic activity of the person proteins is important in disease pathogenesis. We hypothesized; nevertheless, that mutations in digestive nutrients may boost the risk of chronic pancreatitis by an substitute system, which requires mutation-induced misfolding. Intracellular preservation of misfolded meats outcomes in endoplasmic reticulum (Er selvf?lgelig) tension and activates a signaling path aimed in alleviating Er selvf?lgelig burden by raising proteins foldable capacity and attenuating translation.[18C23] Potentially dangerous outcomes of this signaling procedure are the activation of the BMS 378806 inflammatory transcription aspect NFB and the induction of apoptotic cell loss of life. In the present research, the impact was analyzed by us of a consultant CTRC mutant, g.A73T, on Er selvf?lgelig stress apoptosis and indicators in the dexamethasone-differentiated rat acinar cell line AR42J and in major mouse acini. Strategies Recombinant adenovirus structure The cDNAs for the individual outrageous type CTRC and the g.A73T mutant carrying a Glu-Glu epitope label were excised from the previously constructed pcDNA3.1(?)_CTRC phrase plasmids [15,16] with and had been performed by PCR using primers detailed in Supplementary Desk 1. As an inner control for mRNA condition and similar launching, glyceraldehyde-3-phosphate dehydrogenase (splicing in AR42J cells revealing outrageous type CTRC (A) or the g.A73T CTRC mutant (T). Control cells were contaminated with were and eGFP-adenovirus harvested in 24 l. Amounts of CTRC proteins in cell lysates had been examined … Er selvf?lgelig stress in major mouse acinar cells articulating the p.A73T CTRC mutant We motivated mRNA amounts for BiP and XBP1s in freshly ready mouse pancreatic acini using RT-PCR (Fig 5). Both ER stress indicators were elevated in acini expressing the p significantly. A73T mutant compared to cells contaminated with outrageous type CTRC control or pathogen pathogen. We had been incapable to assess intracellular BiP proteins amounts in a dependable way, as mouse acini displayed solid BiP release, which lead in adjustable BiP amounts in cell lysates. The uncommon sensation of BiP release by pancreatic acini provides been reported previously, although its system and physical significance is certainly uncertain.[25] Body 5 ER strain markers in mouse pancreatic acinar cells revealing wild type CTRC, improved green fluorescent proteins (control) or the p.A73T CTRC mutant. (A) Semi-quantitative RT-PCR was performed to determine (398 bp) mRNA amounts. Phrase of … Account activation of the Benefit NFB and path is not observed in acinar cells expressing the g.A73T CTRC mutant Translation attenuation in response to Er selvf?lgelig stress is certainly mediated by the proteins kinase RNA (PKR)-like endoplasmic reticulum kinase (Benefit), an ER membrane layer proteins. Dimerization of Benefit qualified prospects to can boost the capability of chymotrypsinogen C to trigger Er Rabbit Polyclonal to PDLIM1 selvf?lgelig stress and following cell loss of life by a mechanism which is certainly unconnected to the trypsin-degrading activity of CTRC but involves mutation-induced misfolding. Prior research on the system of hereditary risk in persistent pancreatitis led to the ingredients of a trypsin-dependent disease model, which posits that suffered, intrapancreatic transformation of trypsinogen to trypsin has a central function in the advancement of persistent pancreatitis.[2C17] One of the BMS 378806 BMS 378806 defense mechanisms against undesired trypsin activity in the pancreas is certainly CTRC, which was shown to degrade trypsin and trypsinogen with high specificity lately.[15] Mutations in CTRC that abolish catalytic activity and/or reduce secretion possess been found in association with chronic pancreatitis, recommending that failure of the intrapancreatic trypsin-degrading mechanisms increases pancreatitis risk.[16,17] To date, three CTRC mutations possess been confirmed to enhance the risk of chronic pancreatitis statistically. Mutations g.P and R254W.K247_Ur254dun are prevalent in Western european topics whereas mutation g.A73T was present with higher regularity in India. When examined in transfected HEK 293T cells transiently, all three mutants exhibited a release problem as a result of intracellular preservation and destruction apparently.[16] Release of mutant p.Ur254W was reduced by about 50%, whereas mutants g.P and K247_R254del.A73T were secreted just in search for quantities. This common phenotype led all of us to speculate that intracellular destruction and retention of CTRC mutants might cause ER stress, which may contribute to the advancement of.
Research Objectives: Increased cell injury would provide the type of change
Research Objectives: Increased cell injury would provide the type of change in constitution that would underlie sleep disruption as a risk factor for multiple diseases. death; cell proliferation; and concentrations of glutathione peroxidase and catalase. Measurements and Results: Oxidative DNA damage in totally sleep deprived rats was 139% of control values, with organ-specific effects in the liver (247%), lung (166%), and small intestine (145%). Overall and organ-specific DNA damage was also increased in partially sleep deprived rats. In the intestinal epithelium, total sleep deprivation resulted in 5.3-fold increases in dying cells and 1.5-fold increases in proliferating cells, compared with control. Two days of recovery sleep restored the balance between DNA damage and repair, and resulted in normal or below-normal metabolic burdens and oxidative damage. Conclusions: These findings provide physical evidence that sleep loss causes cell damage, and in a manner expected to predispose to replication errors and metabolic abnormalities; thereby providing linkage between sleep loss and disease risk observed in epidemiological findings. Properties of recovery sleep include biochemical and molecular events that restore balance and decrease cell injury. Citation: Everson CA, Henchen CJ, Szabo A, Hogg N. Cell injury and repair resulting from sleep loss and sleep recovery in laboratory rats. 2014;37(12):1929-1940. a purified diet, isocaloric to rat chow at 3.7 kcal/g (modified AIN-76A, Zeigler 382180-17-8 Brothers, Garners, PA). The different treatment conditions and their durations, described in the following paragraphs, are depicted in Figure S1 (supplemental material). The Bergmann-Rechtschaffen experimental apparatus and method are described in detail elsewhere.37,41 In brief, two rats were housed on a large divided platform; each rat occupying one side. The platform could be rotated slowly at a speed of 3.3 rpm. Each rotation was brief, lasting 6 sec, which was sufficient to cause each rat to move in order to remain comfortably on the platform. Baseline conditions included an hourly rotation of the platform but there was no deliberate sleep restriction. Under these conditions, sleep occupies 50C61% of total time.34,41C44 Baseline controls were studied during 7 days of these conditions and compared with the treatment groups in the first set of live animal experiments. Total and partial sleep deprivation were produced for 10 daysa duration known to be sufficient for metabolic changes and mild neutrophilia to become manifest,33,43 but short enough to preclude the advanced morbidity that typically occurs by 18C26 days.34,41,42 To produce total sleep deprivation, the platform was rotated for 6 sec upon detection of sleep onset in one of the two paired rats. There otherwise was no ambulation requirement. Under these conditions sleep is largely prevented and only accumulates to < 10% of total time.34,41C43 Partial sleep deprivation was produced in the rat housed opposite to the totally sleep deprived rat because it experienced the ambulation requirements of the totally sleep 382180-17-8 deprived rat. Under these partial sleep deprivation conditions, sleep C1orf4 is heavily disrupted and occupies 38C44% of total time.34,41C43 Comparison controls in the second set of live animal experiments were subjected to the same amount of disk rotation time as were the partially and totally sleep deprived rats, but rotations of the housing platform were consolidated into periods that 382180-17-8 permitted lengthy opportunities to obtain uninterrupted sleep. Under these ambulation control conditions sleep occupied 51% of total time.44 In different groups of rats, recovery sleep was produced by reinstatement of baseline conditions after the 10-day period 382180-17-8 of total or partial sleep loss to permit a 2-day period of sleep DNA fragmentation by brightfield microscopy (Olympus BX51 microscope and DP71 camera, Center Valley, PA; Image-Pro Plus image analysis software, MediaCybernetics, Bethesda, MD). Brown and dense staining of condensed DNA within the cell was considered positive for 382180-17-8 late-stage cell damage/death. TUNEL-positive cells were counted at 400X magnification in 4 m-thick sections of (1) frozen-embedded spleen (IHC Facility, University of Chicago, Chicago, IL) and (2) formalin-fixed, paraffin-embedded liver, jejunum, heart, and lung (IHCTech, Aurora, CO). The representative regions quantified were 1.6 mm2 of liver or an area that contained at least 1000 cells (0.20 mm2 of spleen and lung; 0.64 mm2 of heart). TUNEL-positive cells in these tissues were expressed as a proportion of the area. TUNEL-positive cells in the spleen were categorized by red or white pulp. In the jejunum, TUNEL-positive cells were counted between the crypt-villus junction and the villus tip in the basal, middle, and apical.
Vasohibin\2 (VASH2) is certainly a homolog of VASH1, an endothelium\derived angiogenesis
Vasohibin\2 (VASH2) is certainly a homolog of VASH1, an endothelium\derived angiogenesis inhibitor. responses to TGF\1 shown by promoter assay and plasminogen activator inhibitor type 1 manifestation were 327-97-9 manufacture significantly attenuated in VASH2 knockdown cells. Furthermore, knockdown of VASH2 in cancer cells abrogated the TGF\1\induced reduced manifestation of epithelial markers including At the\cadherin, and the elevated manifestation of mesenchymal markers including fibronectin, ZEB2, and Snail2, suggesting that endogenous VASH2 is usually required for TGF\1\induced EMT. In accordance with these results, the effects of TGF\1 on cell morphology, migration, invasion, and MMP2 manifestation were also abrogated when VASH2 was knocked down. These results indicate that VASH2 performed a significant function in the EMT by modulating the TGF\ signaling. We offer that VASH2 would end up being a story molecular focus on for the avoidance of EMT in malignancies. rodents are entered with rodents, the number of intestinal tumors are reduced significantly. That reduce is certainly linked with normalization of tumor vessels.19 These results suggest a paracrine impact of VASH2 on tumor angiogenesis. Recently, it was reported VASH2 promotes malignant change through promoting the EMT in hepatocellular carcinoma.20 However, the biological role of VASH2\dependent regulation of EMT has 327-97-9 manufacture not yet been identified. Here we hypothesized VASH2 to be involved in the rules of TGF\ signaling and examined this possibility. Our present study disclosed that the knockdown of VASH2 downregulated the manifestation of ALK5. Accordingly, the TGF\ signaling was significantly attenuated, with 327-97-9 manufacture a reduction in the TGF\\induced EMT, a mechanism that has not been previously recognized. Materials and Methods Antibodies used for immunodetection of proteins 327-97-9 manufacture We used the following antibodies for Western blot analysis: At the\cadherin (3195; Cell Signaling Technology [CST], Danvers, MA, USA), vimentin (5741; CST), fibronectin (610077; BD Biosciences, Franklin Lakes, NJ, USA), ALK5 (sc\398; Santa Cruz Biotechnology, Dallas, TX, USA), Smad2/3 (3102; CST), phosphorylated Smad2 (Ser465/467) (3108; CST), Smad3 (9513; CST), phosphorylated Smad3 (Ser423/425) (9513; CST), \actin (A5441; Sigma\Aldrich, St. Louis, MO, USA), \tubulin (CBL270; Merck Millipore, Darmstadt, Philippines), and hemagglutinin (HA)\tag (M132\3; Medical & Biological Laboratories, Nagoya, Japan). For immunocytochemistry analysis, antibodies realizing At the\cadherin (610181; BD Transduction Laboratories, San Jose, CA, USA), SM22 (ab14106; Abcam, Cambridge, UK), anti\mouse IgG (H+T) Alexa Fluor 488\conjugated (A\11001; Life Technologies) or anti\Rabbit IgG (H+T) Alexa Fluor 594\conjugated (A\21207; Life Technologies, Carlsbad, CA, USA) were used. Cell culture DISS and SKOV3 cells and VASH2 knocked\down (sh\VASH2) clonal cell lines from DISS cells were explained previously.21 Cells were maintained in DMEM (Wako, Saitama, Japan) with 10% FBS LIFR (Sigma\Aldrich) and penicillinCstreptomycin (Wako). For experimental treatments, the serum was reduced to 0.5% and 10?ng/mL TGF\1 (Wako) or 5?M SB431542 (Wako) was added. Immunostaining For confocal microscopic analysis, the siVASH2 and siControl DISS transfected cells, or shVASH2 and sh\control DISS cells, were seeded on coverslips in a 12\well plate at a density of 12??104 cells and cultured overnight at 37C in DMEM with 10% FBS. The next cells were starved for 4?l in DMEM containing 0.5% FBS, followed by treatment with or without 10?ng/mL TGF\1 for 72?l. Cells had been after that set with glaciers\frosty mix of methanol\acetone (1:1) for 30?t, blocked with 1% BSA, and incubated with anti\Age\cadherin (1:200 dilution) and anti\SM22 (1:1000 dilution) antibodies overnight in 4C. The following time, cells had been incubated with supplementary antibodies conjugated either with Alexa Fluor 488 (1:500 dilution) or Alexa Fluor 594 (1:500 dilution) for 1?l in area temperature to visualize stained elements. After comprehensive cleaning, cells had been installed in ProLong Gemstone with DAPI (Lifestyle Technology). The pictures had been captured under a confocal microscope (FV10i; Olympus, Tokyo, Asia). Traditional western mark evaluation Cells had been lysed in RIPA stream formulated with 0.1% SDS (Nacalai Tesque, Kyoto, Asia) and PhosSTOP phosphatase inhibitor drink (Roche, Basel, Swiss). The proteins focus was motivated by the Lowry proteins assay (Bio\Rad Laboratories, 327-97-9 manufacture Hercules, California, USA). Identical quantities of protein had been separated by SDS\Web page and moved to PVDF walls (Bio\Rad Laboratories). The walls had been.
Background Flower viruses such while (CPMV) are increasingly being developed for
Background Flower viruses such while (CPMV) are increasingly being developed for applications in nanobiotechnology including vaccine development because of their potential for producing large quantities of antigenic material in flower website hosts. Findings We found that CPMV is definitely destined and internalized by subsets of several populations of APCs both and following intravenous, intraperitoneal, and oral administration, and also by cells separated from the Peyer's plot following gastrointestinal delivery. Surface vimentin was also indicated on APC populations that could internalize CPMV. These tests demonstrate that APCs capture CPMV particles (1.0 g of computer virus per kilogram of leaves). CPMV, a comovirus that encodes a 31 nm capsid, is definitely a member of the picornavirus superfamily of viruses. CPMV capsids are made up of 60 copies of the large (T) and small (H) protein subunits put together around an RNA genome [16], [18], [19]. Antigenic epitopes are generally displayed by intro of peptide 20069-05-0 sequences in areas of the genome encoding the capsid surface loops. In addition, the surface may become chemically altered for direct attachment of peptides and additional ligands [20], [21], [22], [23]. A variety of bioconjugation methods possess been developed, with surface lysines or launched cysteines most typically utilized. CPMV chimeric viruses showing foreign epitopes from pathogens including HIV-1 [6], [7], mink enteritis computer virus [17], [4], [5] and [3], [5] induce strong humoral immune system reactions against those pathogens or following delivery after oral or intravenous delivery in mice. CPMV offers been extensively analyzed [24], [25]. We shown that following delivery in mice, CPMV was found in virtually all cells for several days after administration [26]. In addition, we showed that CPMV was stable under gastric conditions, and following oral delivery we also observed computer virus throughout the mice suggesting that the computer virus experienced crossed the gastrointestinal epithelium, maybe by connection with Peyer's spots (PP). These secondary lymphoid body organs are located in the airport terminal ileum of the small intestine and consist of dendritic and additional cells of the immune system system. CPMV was also recognized in spleen and lymph nodes, however the individual cell types that interacted with computer virus were not identified [26]. The potential for the use of CPMV and additional flower computer virus particles for vaccine and additional applications require a better understanding of relationships with APCs and following intravenous, intraperitoneal or oral dosing was looked into by circulation cytometry and fluorescence confocal microscopy. Next, the association of CPMV particles Rabbit Polyclonal to GCNT7 with gastrointestinal epithelium and PPs was examined by ileal loop ligation and confocal microscopy. Finally the manifestation of CPMV joining proteins on APCs was evaluated by circulation cytometry. Results APCs Situation and Internalize CPMV Particles In Vitro Several fluorescently-labeled versions of CPMV were made to visualize CPMV by fluorescence microscopy and detect it by FACS. Fig. H1A shows a space-filling model of CPMV with the 300 naturally-occurring revealed lysine residues labeled in reddish, although standard marking using NHS-ester forms of fluorescent dyes yields a maximum of 240 dyes/particle [20]. For most of the tests Alexa Fluor 20069-05-0 488 (AF488) was conjugated to the particles, which offers a stronger and more stable transmission at differing pH. The lysine residues were conjugated with NHS-AF488, obtaining an average of 71.33 dyes per particle to generate CPMV-AF488. For some studies a cysteine mutant labeled with maleimide-fluorescein was used as well. Fig. H1M shows a genetically altered CPMV (vEF) comprising a cysteine residue in each of the large subunits of the capsid [20], and the 60 addressable cysteine residues are indicated in blue. CPMV-vEF particles bioconjugated with maleimide-F were labeled at 23 dyes per computer virus particle to yield CPMV-F. 20069-05-0 The use of either.


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Born on May 4, 1992 in Sarasota, Ashley Nicole Rickards is an American actress probably best known for her role as Jenna Hamilton on MTV's "Awkward." She may also be familiar for her role as Mandy, an autistic girl, in the indie film "Fly Away."
Ashley graduated with honors from high school at age 15 and is a member of MENSA. While in high school, she took Polish as a language. She is also a former competitive equestrian. She said, "I have no idea what a high school party looks like. I was just with my friend and we were walking down Venice and there was this gathering of people playing Bongo drums, and so…we sat down with them…that's the closest I've gotten to a high school experience, meeting strangers and just hanging out with them."
Ashley added, "In elementary school I was unpopular and picked on brutally so I can relate to any of that stuff that happens on our show. In middle school I was popular and then in high school I was so focused on academics that I didn't have time for anything."
Also check out: Photos of 13 Celebrity Nude Scandals
Ashley Rickards is on the board of directors for Project Futures Somaly Mam Foundation, which aims to prevent and end human trafficking.
Her other television appearances include "Everybody Hates Chris," "CSI: NY," "Ugly Betty," "One Tree Hill" and "American Horror Story," among others.
Ashley said, "In my personal life, I become my characters. After 'One Tree Hill,' I started dressing in Converse and ripped jeans and hoodies. On 'Awkward,' it manifests in how I speak. I'm still getting out of it right now."
In her spare time, Ashley Rickards likes to write poetry, scripts and short stories. She has dated musician Tom Cole, and tweeted PDA pictures of her and Tom in bed kissing.
She said the most surprising part of being in Awkward is the amount of fan response. She also joked that she would love to play a female superhero. "I want to be in a leather cat suit at some point in my career. Who doesn't? With a shirtless male co-star. I'm just sayin'."
In 2012, she was seen at the VMAs looking markedly thinner, with blonde hair, which caused speculation she may have an eating disorder.
Ashley Rickards lives in Los Angeles, CA.

subset: commoncrawl


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Welcome Back Desert Bluffs
About This Show
Welcome Back Desert Bluffs is a fan cast based upon Welcome to Night Vale, which is the intellectual property of Night Vale Presents, Joseph Fink, and Jeffrey Cranor. The voice of Terry is Ren Connolly. The podcast takes place in Desert Bluffs, and uses creative commons licensed music for all backing tracks and Climate Reports unless otherwise noted. Email firstname.lastname@example.com with questions, concerns, stone tablets etched with mythic prophecies, suggestions, et cetera.Read more »


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Jan 28, 2014
In an unusual move, Sony has released an original, free-to-play IP that combines fantasy RPG with a game of Blackjack. There are various monsters, tons of equipment, and a story to enrich the gameplay. Suits And Swords can be downloaded for free from here: Suits And Swords on Google Play.


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Two in Nebraska Media Fired After MSNBC Story
KPTM (Cox Channel 10) reporter Calvert Collins was fired Friday, according to station personnel who said they could not comment further.
Collins was the "poster girl" for an MSNBC investigative report this week that identified 143 journalists who made political contributions from 2004 through the start of the 2008 campaign season. Collins was pictured in the article with former Congressional candidate Jim Esch in a photo that appeared on her Facebook page.
At that time, only the political website "The Leavenworth Street Blog" reported on the matter, eventually getting Collins to reveal (in a comment to the original post) that she was "truly shocked how this photo has made such big news. It was an honest mistake, and I should've removed the photo from the website sooner." Collins said the photo of her posing close to Esch was originally posted on her Facebook page in June and removed in late October.
In the MSNBC story, Collins told reporter Bill Dedman that her father made the $500 contribution to Esch's campaign.
A Google search for mentions of Collins' campaign contribution turned up more than 250 stories, many of them also mentioning "KPTM" and "Fox" within the text.'
KPTM station managers could not be immediately reached for comment Friday evening. News Director Joe Radske, however, was not working at KPTM when the matter in question originally came to light in late October of 2006.
And She Wasn't The Only One
Collins was not the only Nebraska-based media member to lose their job over Dedman's story.
Lincoln Journal Star editorial cartoonist Paul Fell uses profanity in his reply and lashes out at the newspaper when asked about his $450 contribution to Maxine Moul, Democratic candidate for the House.
Wrote Fell in an e-mail to Dedman:
"They don't pay me enough money to be able to dictate how I conduct myself in political campaigns. I generally do not donate to political candidates, but Maxine Moul is a longtime friend and former newspaper publisher where I got my start as a cartoonist back in 1976.
"Frankly, I don't give a rat's ass what the Lincoln Journal Star or their parent organization, Lee Enterprises, policies are on allowing newsroom staff to give to candidates and parties. I do not believe they did disclose my donations. That's their problem, not mine."
In a story published in Saturday's editions of the Journal Star, Editor Kathleen Rutledge acknowledges that Fell was one of two employees of the newspaper mentioned in Dedman's story.
Copy Editor Sylvia Hermanson made a $250 contribution to the Democratic National Committee in January, according to MSNBC. Rutledge said Hermanson (who asked Dedman if she was "busted") notified management when MSNBC contacted her for comment. Rutledge said Hermanson was reprimanded.
Fell, though, did not tell Journal-Star newsroom managers about his political contribution at the time he made it or when he was contacted by MSNBC. Rutledge took particular issue with Fell's comments to Dedman, saying Fell made it clear he did not care about the newspaper's trust with its readership.
"We don't think he should treat our credibility with such disdain," Rutledge writes. "His comments to MSNBC follow an earlier incident in which he did not disclose to us that he had posted cartoons of a sexual nature on his Web site. He removed them after we expressed concern."


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Kim Kardashian Visits Orphanage in AfricaKim Kardashian4:07 pm, January 25th, 2013
PHOTOS(GettyImages.com)While recently in the Ivory Coast, Kim Kardashian visited Orphanage Case Blanche."We had some time off in Abidjan so we wanted to visit an orphanage and we brought them tons of food and toys!" the reality star wrote on her blog on Friday.She added, "The kids sang and danced for us and it was such a magical experience! They were the sweetest kids and I'm so glad I got to visit during my short trip."Of course, hanging out with little children was also some good practice for Kardashian — she and Kanye West are expecting currently their first baby.CHECK OUT photos from Kardashian's orphanage visit here!Follow @GossipCop on Twitter!Like us on Facebook!
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Celebrity Booty Mishaps
Jay-Z: Blue Ivy May Be The "Worst"
Do You Recognize This Nerdy Celeb?
Zac Efron Wears Earmuffs, Looks Ridiculous


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Good Witch Season 1Eps10
Cassie Nightingale's and her daughter Grace's charm is no match for Middleton's newest neighbors.
Reign Season 4Eps16
Queen Mary, unsure who to trust, tests her brother James to ensure his loyalty after hearing of his meeting with John Knox. Queen Elizabeth agrees ...
Good Witch Season 3Eps5
"Good Witch" will take viewers on a new magical journey with Cassie Nightingale and her daughter Grace. When Dr. Sam Radford moves in next door ...
The Bold Type Season 2Eps10
The Bold Type Season 2,The Bold Type Season 2 2018


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This list is by no means exhaustive – but hopefully these will be some of the most useful for learning the tools and giving ideas how to use them for teachers.
http://sites.google.com/site/gtaresources/events/2010-07-29 The Google Teacher Academy resources - there are some amazing resources here – it is going to take me ages to look through them all, there are so many useful videos and ideas. Also the people that went on the Google Teacher Academy yesterday may start blogging and add their ideas. If so I will try to list them as well.
http://sites.google.com/site/gtaresources/events/2010-07-29/apps Google Apps for Education
Get Google apps for your school http://www.google.com/a/help/intl/en/edu/index.html
Google Apps training centre http://edutraining.googleapps.com/
Google Docs http://echucaelearning.wikispaces.com/Google+Docs
Screen Casts and Ideas
http://www.vimeo.com/album/18311 - a set of five videos, calendar, mail, talk, docs and opening page
http://sites.google.com/a/nausetschools.org/googlegoodies/ screencasts includes forms and making a Google website
A whole Google search result
24 Interesting ways to use Google Docs in the Classroom http://www.ideastoinspire.co.uk/googledocs.htm
Sketchup - http://sketchup.google.com/industries/edu/primary.html
Video Tutorials http://sketchup.google.com/training/videos.html
Self paced tutorials http://sketchup.google.com/3dwarehouse/cldetails?mid=36e1fa0d054a15eecc725c514c21d975&prevstart=0
Primary school projects https://docs.google.com/Doc?id=dc837t9h_12g2pxr6
Geo Education Google Earth & Google Maps
http://www.google.com/educators/geo.html Google maps, Earth, Sky and incorporating Sketchup
Google Earth for educators http://sitescontent.google.com/google-earth-for-educators/
Google Maps Mania http://googlemapsmania.blogspot.com/2006/08/educational-uses-of-google-maps.html
25 interesting ways to use Google Earth in the Classroom http://www.ideastoinspire.co.uk/googleearth.htm
Using Google Maps in Education including tutorials http://www.warrensburgr6.org/education/components/scrapbook/default.php?sectiondetailid=382
Blogger http://www.google.com/educators/p_blogger.html
How to use Blogger http://www.docstoc.com/docs/4107328/How-to-use-blogger
Using Blog to integrate Technology in the classroom http://www.glencoe.com/sec/teachingtoday/educationupclose.phtml/47
Picasa in Education http://picasained.wikispaces.com/
How can Picasa be used in Education https://wiki.itap.purdue.edu/display/Social/Examples+of+Picasa+in+Education
If anyone knows or more resources that schools really need to enable them to use the Google Tools in the classroom please do add them as comments, or e-mail them to me email@example.com and I will add them to the post.


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During its history, Charente-Maritime has inherited an exceptionally rich and varied maritime heritage, lovingly maintained and displayed by individuals, associations and organisations.
The Charente coastline and the surrounding islands offer a range of diverse and impressive fortifications, including Fort Boyard, and a few exceptional lighthouses, a unique way for visitors to appreciate even further the environment of the region. It is also the occasion to discover and learn about the myriad of sailing boats that make up the local fleet, both for fishing and pleasure boating.
Ever since the Middle Ages, the local population has used traditional methods to use the resources offered to them by the coast, whether it be oyster farming, mussel rearing or salt farming. There are also fishing techniques that demonstrate specific skill and knowledge, such as the installation of carrelets (fishing huts on stilts) or the construction of fish locks.


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Trainings and Events
You have questions? Need a training? Want to meet other developers at an event?
|MBS Xojo Training
|5th September 2018
|Munich, Germany
|MBS Xojo Conference
|6th/7th September 2018
|Munich, Germany
|Xojo Developer Conference
|1st to 3rd May 2019
|Miami, Florida
Personal Training
You have a couple of questions related to development in Xojo? Problems with a project? You want to learn some best practices in development? Or a few new things you never tried before?
Get started in databases? Learn how to use graphics, xml or sockets? Or some advanced language features like delegates, declares, extends, variants, interfaces or operators?
Please ask us for your training day and prepare a list of questions or topics so we can plan your training.
Location: Your office, our office or some other place like a hotel.
Language: German or English.
Requirements: Computer with Xojo.
Cost: 699 € per day. Add travel, hotel and VAT if needed. Add 100 € for each extra trainee.
Please call us for a detailed quote.
If you visit us in our office for the training, we'll gladly find you a hotel room nearby.
Personal Remote Training
The personal training above is also available via video chat.
Perfect for some urgent problems or quick questions.
You share your screen with Teamviewer or Skype so our trainer can see your screen and control mouse and keyboard if needed.
Location: anywhere.
Language: German or English.
Time: During office hours in Germany.
Cost: 99 € per hour, 249 € for 3 hours. Only one trainee.
Please note that quality of video and audio may be limited due internet connection.
MBS Xojo Chart Plugins


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Bobcats beat Magic for second win in last three games
ORLANDO -- The Charlotte Bobcats didn't look like the worst team in the NBA Tuesday night.
The Orlando Magic did. And they might soon have a lock on that unwelcome distinction.
The battle for the NBA's worst record got a little tighter Tuesday after Gerald Henderson and Kemba Walker each scored 24 points to lead the Bobcats to a 105-92 victory over the Magic.


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Since 2008, the philanthropic landscape in Rhode Island has had a slow recovery. More than 90,000 households — including 230,000 people – in Rhode Island are struggling to meet basic needs. And nearly 20% of Rhode Island children live in poverty.
The United Way's State of the State data dashboard is a snapshot of data and research from education, economic and financial stability, and health and well-being. As you navigate through these data, please note that they represent merely a sampling of the many complex issues, challenges, and opportunities facing Rhode Islanders today. There are many ways to look at and aggregate information. We are offering one viewpoint and endeavor to provide as much context as possible should you wish to dive more deeply or see an alternative point-of-view.
To provide you with the most informative experience, you will find links to our glossary page explaining some of the jargon, as well as embedded links to outside research and data sites that offer more in-depth information on the data depicted in UWRI's dashboard.
State of Economic Security
Rhode Islanders regularly have to choose between putting food on the table and keeping the lights on, or paying rent or paying for that doctor's visit. Read more.
State of Lifelong Learning
Learning is a lifelong pursuit, and a pursuit that has become increasingly necessary for success in the 21st century workforce. Read more.
State of RI Health
Chronic illness and access to preventive medical and mental health care continues to be a pressing issue for many Rhode Islanders. Read more.


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Hey there! I have been trying to come up with a cool accent piece to go in the corner of the walkway between my living room and kitchen and I came up with this easy and FREE Rustic Corbel!
Build this EASY Rustic Corbel with scrap wood! Love it!
Tuesday, 24 March 2015
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On Valentine's Day, Lucky 32 for lunch
' firstname.lastname@example.com
Only a true cad would combine business with pleasure on Valentine's Day, but that's exactly what I did on Feb. 14 when I took my beloved to one of Greensboro's most revered culinary institutions for a romantic lunch.
Dinner on Valentine's Day can be a frustrating exercise, what with the reservations, the special menus, the harried servers and inexperienced patrons. It took me more than a decade of marriage to realize that lunch was the way to go on days like this, with one caveat: You've got to go first-class.
Lucky 32 fits the bill nicely, a clean and elegant eatery with a locally sourced menu and dishes prepared by one of the Triad's best chefs, Jay Pierce, who came all the way up from New Orleans just to make our mouths water — fitting, because I met my bride in the Crescent City on the best spring day of my life.
The offerings at Lucky 32 changes with the seasons; the Mid-Winter Carnival menu marked our visit, a selection of Creole-inspired dishes to go along with a host of interesting options.
Lamb sliders? Pig Bread? The Carolina Cobb? They all looked tempting, but I could not turn from the carnival menu, which boasted boudin, eggplant creole and jambalaya, among other items. I went simple: Though the oysters served on a bed of creamed spinach tempted me mightily, I went with the fried-oyster salad, , complemented by a cup of gumbo. My date went with the grilled escolar. More on that in a minute.
I make gumbo, you know — it's taken me years to learn patience with the roux, the uniformity with which the Holy Trinity (celery, onion and bell pepper, bruh) must be chopped, the mix of spices that bring enough heat to wake up the palate without searing it completely numb. I was pretty happy with my gumbo… until I tried Pierce's.
The darkened roux makes the broth a magnificent chocolate brown, the ingredients pile up above the rim of the cup, the aroma is enough to send one into a full-on swoon. He's got okra in there, shrimp… and was that a crawfish tail? Maybe so. The flavor is richer, the spicing more nuanced, the overall appearance and taste such that my own gumbo seems closer to actual mud.
The oysters were pretty good too, flash-fried in a cornmeal batter, served atop a bowl of baby spinach with some chopped red onion, a little goat cheese and a simple vinaigrette. A dish like this one relies so much on quality ingredients and the flavors they bring that it needs no flourishes or embellishments.
As for my wife, her escolar made her happy. Escolar — an ugly, deep-water fish — rates very highly on the omega-3 scale, making it a very healthful fish. It's white, not too fishy and has a steak-like texture which enables it to hold a char very nicely. But those omega-3s make the escolar a dangerous fish to consume if you haven't the constitution for it. At a seafood restaurant where I used to work, the chef referred to it as "escolax."
My wife, whose health is unparalleled by any other human I've ever met, could probably digest a piece of firewood. She had no problems with it.
Then it was on to dessert. Lucky 32's dessert menu is a local treasure, all made in house with local goods. We looked at the red-velvet cupcakes, the buttermilk and chocolate chess pies, the scuppernong wine jelly cocktail. But we settled on the bread pudding, with flavors of white chocolate and banana. In honor of our relationship, which began so long ago in the city of New Orleans, it seemed like the right thing to do.
WANNA go?
Lucky 32 Southern Kitchen; 1421 Westover Terrace, Greensboro; 336.370.0707; lucky32.com


text:
PUB 700333: Governor and Lieutenant-Governor; Separate Primaries; Joint Election by Loyal Millett
ARTICLE IV: EXECUTIVE, Section 5. Election of governor, lieutenant governor and cabinet members; qualifications; terms.
SECTION 5.Election of governor, lieutenant governor and cabinet members; qualifications; terms.
- At a state-wide general election in each calendar year the number of which is even but not a multiple of four, the electors shall choose a governor and a lieutenant governor and members of the cabinet each for a term of four years beginning on the first Tuesday after the first Monday in January of the succeeding year. In primary elections, candidates for the office of governor
may choose to run without a lieutenant governor candidate.In the general election, all candidates for the offices of governor and lieutenant governor shall form joint candidacies in a manner prescribed by law so that each voter shall cast a single vote for a candidate for governor and a candidate for lieutenant governor running together.
- When elected, the governor, lieutenant governor and each cabinet member must be an elector not less than thirty years of age who has resided in the state for the preceding seven years. The attorney general must have been a member of the bar of Florida for the preceding five years. No person who has, or but for resignation would have, served as governor or acting governor for more than six years in two consecutive terms shall be elected governor for the succeeding term.


text:
8:58 (Paul Hartnoll, Orbital) – 8:58 [Deluxe Edition] (2CD) (2015)
FLAC (tracks+.cue) | Tracks: 19 | Playtime: 00:48:58+00:53:19 | Size: 632.5 MB
Genre: Downtempo, Electronic, Techno, Ambient | Label: ACP Recordings | Cat#: ACPCD1502
8:58 is the newly christened reputation for the part scheme of ORBITAL's Paul Hartnoll. There are explicit twines running finished this record which inevitably hear tail to ORBITAL, the sequential subject of the denomination slot plus 'The Cuckoo' repeal the 'Planet of Appearances' from 'The Dark Anthology' which featured the "Even a stopped date recounts the prerogative epoch twice a date" test from 'Withnail further I'.
The record opener furthermore appellation rut physiognomys 'Star Trip Against Night' besides 'Peaky Blindfolds' luminary Cillian Murphy intoning about how everywhere our dwells, we are grinds to the unstoppable ticking of time further the chronometer. Murphy linked up concomitant Hartnoll during the final was commissioned to fabricate the soundtrack for the BBC Gangland-based TV series alongside beside PJ Harvey further the fabulous Flow (who wields mixing duties here besides). Murphy too occurs in the accompanying promo movie which landmarks a 'Mr. Benn' merchant-way cameo from Paul Hartnoll himself.
01. 8:58 (Featuring Cillian Murphy) 6:05
02. Please (Featuring Robert Smith & Lianne Hall) 6:35
03. The Past Now (Featuring Lisa Knapp) 6:36
04. Villain (Featuring Ed Harcourt) 3:51
05. The Clock (Featuring Cillian Murphy) 3:09
06. A Forest (Featuring The Unthanks) 5:43
07. Broken Up 4:21
08. Nearly There 5:02
09. Cemetery (Featuring Fable) 7:37
01. 8:58 (Instrumental) 6:27
02. Please (Instrumental) 4:58
03. The Past Now (Instrumental) 6:35
04. Villain (Instrumental) 3:50
05. The Clock (Instrumental) 3:05
06. A Forest (Instrumental) 5:33
07. Broken Up 4:20
08. Nearly There 5:01
09. Cemetary (Instrumental) 7:36
10. Risky 5:52
Lossless music 8:58 (Paul Hartnoll, Orbital) – 8:58 [Deluxe Edition] (2CD) (2015) FLAC (tracks+.cue) download flac
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text:
The Sports Xchange
2012 Training Camp Preview: Cincinnati Bengals
Bengals training camp preview
The Sports Xchange
2012 Training Camp Preview: Cincinnati Bengals
Bengals training camp preview
1. Find a second receiver. We all know what A.J. Green can do after his outstanding rookie season, but if the Bengals are going to take that next step as an offense, they need someone opposite him to emerge. Most have third-round pick Mohamed Sanu as the favorite because he is a physical receiver that the Bengals are lacking and can open up the short- to medium-passing game that was missing last season. Former University of Cincinnati standout Armon Binns continues to impress the coaching staff with his work ethic plus his ability to go up and make the difficult catches. The dark horse though is Brandon Tate, who averaged over 18 yards per catch with New England two years ago. Tate was mainly used as a returner last year.
2. Sorting out the secondary. When Leon Hall tore his Achilles in last year's Week 10 loss to Pittsburgh, many thought that he wouldn't be back until this midseason. But Hall's rehab has gone so well that he expects to be on the field for the first practice on July 27. Hall and Nate Clements are expected to be the starters at corner but the bigger problem is at safety where no one has stepped up to fill the void left after the release of Chris Crocker. Taylor Mays is the favorite to eventually win the spot, but he remains inconsistent in coverage. If Mays falters, either Clements or Jason Allen could move to safety which would open the way for first-round pick Dre Kirkpatrick to start at corner.
Running back BenJarvus Green-Ellis, who signed with the Bengals after four years in New England. The Bengals want to go with a backfield by committee approach and that is best suited for Green-Ellis and Bernard Scott. Green-Ellis does not have the breakaway speed you would like in a back, but he does get the tough yards and excels in the red zone, which is two areas of the run game where the Bengals have struggled mightily over the past two seasons. Green-Ellis wasn't asked to catch the ball much out of the backfield with the Patriots, but he did a lot of it during offseason workouts with the Bengals. Green-Ellis is also more suited to a committee approach, which wasn't the case last year with Cedric Benson. He also never fumbled during his four seasons in New England.
Linebacker Rey Maualuga, who is going into the fourth and final year of his contract. Maualuga moved back to his original position of middle linebacker last season and struggled. He was good in calling the plays and getting the defense ready, but there were many cases where he over-pursued a guy or was in the wrong spot. If Maualuga is going to show that he can be a leader in this defense and a consistent player, it has to come this year. Another thing that worries the Bengals if they were to make a long-term commitment to Maualuga is that he has been involved in off-field incidents two of the last three offseasons.
Lewis likes what he learned during offseason
--Coach Marvin Lewis will enter the upcoming season third for most regular-season games coached without a playoff win behind Jim Mora (231) and Norm Van Brocklin (173).
Lewis, who is going into his 10th season, has coached in 144 regular-season contests and has a career record of 69-77-1, which includes three postseason losses.
--With minicamp complete and players not reporting to training camp until July 26, Lewis was pleased with how things went in the offseason program.
Said Lewis in summing up the offseason: "We're a bigger football team. I believe we're a faster football team. The depth and development of our young guys has been impressive. That's what will make it a competitive camp. The exciting thing is when we get out there against the Jets and open things up in the preseason, we'll have a lot of guys playing for keeps."
Quarterback Andy Dalton also echoed his head coach's sentiment and noted that players on offense are picking up quicker on things and that the communication is better.
--While there is still a lot of training camp practices and four preseason games to go, Lewis did say this is the best group of undrafted rookies that he has had in his 10 seasons in Cincinnati. In 2009, three undrafted rookies -- wide receiver/punt returner Quan Cosby, safety Tom Nelson and linebacker Dan Skuta played key roles.
The two that have been the most impressive early are linebackers Emmanuel Lamur and Vontaze Burfict. Of all the position groups, linebacker might be the easiest for an undrafted guy to make it because of the lack of depth.
--The first two days of Bengals training camp could be a logistical nightmare. Most of the practices during camp will be held on a side field but on July 27-28 the Macy's Music Festival will be held inside Paul Brown Stadium.
The team has yet to announce how many of the practices will be open to the public or how many will be in the stadium.


text:
Legal Status
Document Details
Published Document
The Department of Commerce will submit to the Office of Management and Budget (OMB) for clearance the following proposal for collection of information under the provisions of the Paperwork Reduction Act (44 U.S.C. Chapter 35).
Agency: National Oceanic and Atmospheric Administration (NOAA).
Title: User Engagement Survey for Water Resources Forecasts and Climate Information.
OMB Control Number: None.Start Printed Page 12693
Form Number(s): NA.
Type of Request: Regular submission (request for approval of a new information collection).
Number of Respondents: 90.
Average Hours per Response: 30 minutes.
Burden Hours: 45.
Needs and Uses: This request is for approval of a new information collection. The National Weather Service plans to conduct a survey to engage with and assess the science and forecasting needs of stakeholders in the water resources sector. The water resources sector includes agencies and companies operating reservoirs, and private and public interests in regulating rivers. The survey is designed to (1) assess the accessibility and utility of water and climate information and data, (2) assess participants' perceptions and knowledge about water and climate, and (3) evaluate user needs and the gaps in existing water and climate information. Participation in the survey will be entirely voluntary and will usually be in conjunction with workshops related to water resources and/or climate.
Affected Public: State, local and Tribal government; individuals or households; not-for-profit organizations.
Frequency: Annually.
Respondent's Obligation: Voluntary.
OMB Desk Officer: firstname.lastname@example.com.
Copies of the above information collection proposal can be obtained by calling or writing Diana Hynek, Departmental Paperwork Clearance Officer, (202) 482-0266, Department of Commerce, Room 6616, 14th and Constitution Avenue, NW., Washington, DC 20230 (or via the Internet at firstname.lastname@example.com).
Written comments and recommendations for the proposed information collection should be sent within 30 days of publication of this notice to firstname.lastname@example.com Signature
Dated: March 2, 2011.
Gwellnar Banks,
Management Analyst, Office of the Chief Information Officer.
[FR Doc. 2011-5122 Filed 3-7-11; 8:45 am]


text:
SOURCE: Gracenote
January 07, 2008 08:00 ET
New Solution Combines Industry-Leading Content Identification System, Proven Network Services Infrastructure, and the Largest Database of Film and Television Works Ever Created
LAS VEGAS, NV--(Marketwire - January 7, 2008) - CES -- In 2007, consumers' consumption of
digital video became ubiquitous -- from video blogging to online
advertising, from user-generated mash-ups to major studio distribution,
from the cell phone to the back-seat of the car. However, creating both an
enjoyable consumer experience and a viable business around digital video
remains a challenge, and Gracenote® is introducing a comprehensive Video
Platform that both enhances video enjoyment and provides a holistic set of
tools for video management across every digital video medium. The new
Gracenote Video Platform will give consumers, device manufacturers, and
content owners the most effective way to search, identify, manage, and
enjoy expanding video collections when accessed from their computers,
mobile phones, DVD players, and other consumer electronics devices.
Recognized as the worldwide leader in digital music identification services
for the last decade -- with more than two billion searches per month
against its database by more than 300 million annual unique users --
Gracenote is expanding its digital media product line with a sophisticated,
end-to-end solution for identifying, enjoying and managing video content
unlike any other created. The new Video Platform combines Gracenote's
Global Media Database with exhaustive data from partners including the
British Film Institute (BFI) catalog of data on feature films, TV episodes,
documentaries and animated films; and Stingray, a video data provider for
the Japanese market. The Gracenote database will be the largest catalog of
video works ever complied and will provide detailed, rich data for over
300,000 DVDs and nearly one million total unique video programs.
The company's new Video Platform features five new solutions: VideoID-DVD,
VideoID-File, Video Link, a video recommendation engine, and a media
monitoring and monetization system, all of which are planned to be launched
in phases over the next 18 months. The suite of solutions is designed to
provide consumers, device manufacturers, and content owners an integrated
system that not only recognizes video, but supports a wide range of
complementary applications, such as the delivery of enhanced metadata and
cover art to enrich consumers' enjoyment of their DVD collections, the
ability to make purchase recommendations based on a consumer's existing
video library, and the performance of video identification to help content
owners monetize their video content viewed online. Gracenote is
demonstrating the new Video Platform for the first time at the Consumer
Electronics Show (CES) in Las Vegas this week.
"The Gracenote Video Platform strikes at the center of what many consider
to be the number-one shift in media consumption: online video," said Craig
Palmer, Gracenote CEO. "Today, there isn't an effective way of identifying,
tracking, and monetizing the millions of digital video files being created,
accessed, and shared across multiple platforms. At the same time, consumers
are often forced to wade through video they don't like and stumble upon
video they do like without the opportunity to more easily search and find
video, purchase related products or services, or enhance the experience in
any meaningful way. Gracenote's Video Platform addresses every one of these
challenges and provides the necessary tools to support the industry and the
phenomenal growth trajectory it's riding."
Gracenote's Video Platform
Gracenote's Video Platform includes five products -- all in one powerful
technology solution that can be customized for various usage scenarios:
-- VideoID-DVD: Enables identification, search and organization of
physical DVD, HD-DVD, and Blu-Ray disc collections, and provides metadata,
cover art, synopsis, cast/credits, and detailed product information for
identified discs.
-- VideoID-File: Identifies video files or streams using proprietary
video fingerprinting technology to recognize the content. It supports a
number of applications, such as content monetization using Gracenote's
Video Content Manager, and organization of a consumer's time-shifted
broadcast TV recordings. VideoID-File can be implemented in software or in
devices that can access video files or streams.
-- Video Link: Expanding on Gracenote's current audio content linking
technology, this solution enables content distributors to partner with
device manufacturers to link a device's playback of a video title to
related content. Video Link supports the delivery of dynamic and updatable
content such as newer/refreshed trailers, bonus footage, interviews, and
deleted scenes, provides a platform for interactive content to build fan
communities; and enhances retail opportunities for discs, games, toys,
theatre tickets, and more.
-- Video Recommendation Engine: Recommends video titles for VideoID users
based on genre, keyword, era, director, cast, and a host of other
variables. Recommendations can be based on selected video and users can
search across a variety of categories themselves.
-- Media Monitoring and Monetization System: Adding to Gracenote's
existing market-leading audio capabilities, this system is designed to
allow content owners to tag, monitor, manage, filter, and monetize video
content by identifying video files and streams online using VideoID-File.
Content owners can identify their copyrighted content being uploaded to or
streamed from Internet Web sites and then associate particular usage rules
for the identified content as part of its agreement with the Web site
"The studios want to work with a small number of technology providers who
can deliver a complete solution, including the video identification
technology, comprehensive video metadata, and the expertise of managing an
online global media database," said Brad Hunt, the former CTO of the MPAA
and now digital media industry consultant. "Gracenote, whose video
fingerprinting technology was one of the top performers in the MPAA content
recognition testing, is uniquely positioned to fill this role."
Addressing Consumer, Manufacturer and Content Owner Needs
Gracenote's Video Platform is the only comprehensive solution that enriches
the consumer experience, significantly enhances device manufacturer product
lines, and provides a holistic set of tools for content owners:
-- Consumers: Consumers are looking for ways to more easily search their
personal DVD collections, broadcast TV recordings, and Web-based video
purchases, and they want access anytime, anywhere: on the couch in front of
the TV, at the computer, on the cell phone, on their portable media
players, and in the backseat of the car. Gracenote's VideoID-DVD and
VideoID-File will give consumers the ability to enjoy their video
collections with enhanced metadata viewable on any device or Web site.
Consumer will be able to virtually flip through their DVD disc collections
via their cover art images, read the synopsis, discover the actors, receive
recommendations for other films, etc. -- from any device, anywhere,
-- Device Manufacturers: Gracenote can deliver its advanced video
recognition and content metadata linking capabilities to any platform where
consumers enjoy video -- across set-top boxes, disc players, portable media
players, game consoles, PC applications, media servers, cell phones, Web
sites, and automotive disc players. VideoID-DVD, VideoID-File, Video Link,
and Video Discover provide a powerful set of capabilities with advanced
video search, purchase, playback, and recommendation features built-in.
-- Content Owners and Distributors: Far more than the ability to track
and manage enormous volumes of content, rights holders need tools for
content distribution, cross-promotion, up-sell, advertising-supported,
revenue sharing, payment, etc. -- and not just via a computer but across
every consumer device that can access video content. Gracenote's Video
Content Manager leverages the company's powerful audio and video
fingerprinting technology combined with its extensive Global Media Database
and management system that allows content owners to identify use of their
content across various channels and specify actions to take for each
distribution method.
A complete and effective digital content management system requires three
equally important capabilities for a compelling solution: effective content
identification technology, extensive content metadata, and dependable
Global Media Database service. Gracenote is the only provider with all
three capabilities.
Gracenote's Proven Technology
The Video Platform combines Gracenote's proven media recognition system,
MusicID® -- used in nearly every kind of device and with major rights
management and broadcast monitoring customers across the world -- with new
video fingerprinting technology to provide accurate identification of video
and audio content, even when the content has been intentionally distorted
in an attempt to avoid detection. Gracenote's combined audio / video
fingerprinting solution was recently tested by the MPAA and performed well
above other solutions. The video identification technology pairs with a
flexible content management platform to provide filtering, monitoring, and
monetization solutions for content owners.
Gracenote combines this audio / video fingerprinting with its other
technologies including disc recognition, metadata delivery, PC and device
SDKs, and Web technologies to create its best-of-breed Video Platform. With
the Video Platform, Gracenote can identify video discs, files, and streams,
typically within a few seconds. Combined with its existing audio
fingerprinting technology Gracenote has unmatched capabilities to deliver
combined audio and video recognition across film, television, and
commercial music.
Gracenote's Extensive Content Catalog
The Video Platform leverages an exclusive arrangement where Gracenote has
combined its own information on film and television with content from BFI,
Muze and Stingray, resulting in the broadest, deepest database of film and
television works ever assembled. The BFI database features comprehensive
data on more than 800,000 film and television works worldwide, while
Stingray boasts a deep database of more than 100,000 film and television
works across the U.S., Europe and Japan. The partnership with Muze delivers
the most complete data on virtually every DVD, HD-DVD, and Blu-Ray disc
across the US and UK, including cover art, synopsis, reviews, and more.
Far more than providing a video's title, actors and director, Gracenote's
Video Platform dives into more than 50 data attributes on hundreds of
thousands of feature films, including cover art, cast members, production
crew, studio, genre, keywords, language, interactive elements, and links to
related products, photos, Web sites and more. This rich and extensive
archive of audiovisual works is combined with Gracenote's industry-standard
commercial music database to create a truly Global Media Database with the
deepest, most relevant, most comprehensive coverage across film,
television, and music.
Applying Proven Expertise in Music
Gracenote manages the number-one service on the planet for managing music
content metadata, and sets the standard for high availability and
dependable service within the entertainment industry -- features that are
critical to any real-time video identification, metadata delivery, and
content management service. Digital music is in abundance everywhere, and
wherever it is, Gracenote provides the metadata, content identification,
cover art, lyrics, and host of other services to consumers, device
manufacturers, and content owners. Gracenote is extending its expertise to
deliver the same level of quality, performance, and dependability to the
video entertainment industry.
Gracenote at CES
Gracenote will demonstrate the new Video Platform at CES in the South Hall
3, Booth 31453. To schedule a meeting with Gracenote at CES, please contact
Veronica Skelton, 415-342-3435, or email@example.com. For more
information on Gracenote's Video Platform or host of digital music and
video services for the entertainment, mobile, automotive, and consumer
electronics industries, please visit www.gracenote.com, or contact us
directly at firstname.lastname@example.com.
About Gracenote
Gracenote is a global leader in embedded technology, enriched content, and
data services for digital entertainment solutions within the Internet,
consumer electronics, mobile, and automotive markets. Formerly known as
CDDB®, Gracenote delivers a substantially improved consumer experience in
digital media devices and applications, plus media monitoring and other
data services to the recording industry, making it an integral part of the
digital media economy. Gracenote powers leading services including Apple
iTunes, Yahoo! Music Jukebox, Winamp; home and automotive products from
Alpine, Panasonic, Philips and Sony; and mobile music applications from
Samsung, Sony Ericsson, KDDI (Japan), KTF (Korea), Musiwave (Europe), and
others. Headquartered in Emeryville, California, Gracenote has offices in
New York, Tokyo, Berlin and Seoul. For more information about Gracenote,
please refer to www.gracenote.com/corporate/.
Gracenote and MusicID are registered and Mobile MusicID is a trademark of
Gracenote. All other names are trademarks and/or registered trademarks of
their respective owners.
For More Information Contact:Veronica SkeltonConcept Communications for Gracenote415.342.3435Email Contact
See all RSS Newsfeeds


text:
This contest has ended.
Ladies and Gentlemen, I'm in love.
Oh, with my husband and children, of course! But there is a new love in my life- by the name of Scentsy.
Now, I am a candle kind of girl. I have them in every corner of my house, and I have many of them on display! I love all shapes, colors and scents of candles! But there are some things about Scentsy that are very, very different!
First, you don't need to light anything to burn this candle! You are simply burning scented wax over a light bulb! Therefore, there is no smoke, no harmful chemicals, no worries over fires from spills... they are simply low maintenance! They are leadless, wickless, flameless and smokeless!
Another thing I really love about Scentsy is the fact that their products quickly fill my entire home with fragrance, yet they are not at all overpowering! They emit a subtle, yet definite scent. The bulb that is included in their warmers can be replaced at an everyday hardware store!
Take a look at Kara Egan's (Scentsy Founder and Superstar Director) website! You will fall in love with their burners! The scents are absolutely heavenly. I wish you could smell them through the website!
Trust me, readers. You really want to win this giveaway!
Kara is offering to one lucky winner an August warmer of the month, entitled "Surfs Up," along with a 3 pack of bars including the new scent of the month, "Tangerine Tango" and two others!
To enter this giveaway...
To enter, please do any or all of the following (please leave separate comments for each, so you can enter multiple times!):
1. Comment below, with your email address, and tell me if you've tried Scentsy before!
2. Visit Kara Egan's Scentsy site by clicking HERE, and leave a comment telling me which burner is your fave!
3. Subscribe to this blog (enter your email address in upper right corner), and comment that you do.
4. Follow this blog (in right hand column), and comment that you do.
5. Become a fan of this blog on Facebook by clicking HERE, and comment that you do.
6. Email this contest out to your friends, and copy me on it at email@example.com
7. Follow me on Twitter (vaagen) and ReTweet this contest on Twitter: http://twitter.com/vaagen/statuses/2765898678. Comment below that you did.
8. Favorite this blog on Technorati by clicking HERE
The winner will be chosen by random drawing on http://www.random.org/integers/ on Saturday, August 1st at 6pm. Good luck!
|Tags: The Vaagen 6 SCENTSY!!!
|Rating: 0.00 (0 votes) Rate this article
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text:
Citizen Women's EW1710-56Y Elektra Eco Drive Watch
Citizen Women's EW1710-56Y Elektra Eco Drive Watch
Product Description
The company was established in 1918. The founding fathers selected the name Citizen so it would be "Close to the Hearts of People Everywhere" and soon after adopted the company's formal name, Citizen Watch Company.
During the last seventy-five years Citizen has expanded its business throughout the world and has achieved recognition as the global brand. The past twenty-five year period has coincided with the company's dramatic rise to its current position as the world's largest watchmaker, a distinction Citizen has held every year since 1986.
Beyond sheer size, Citizen is also recognized as a worldwide leader in advanced technology. From the world's slimmest LCD watch to the first voice recognition watch and the world's first professional dive watch with an electronic depth sensor, Citizen's record of "world's firsts" is unmatched.
More recently, Citizen has staked out a new position as the leader in timekeeping with its Eco-Drives watches that are light powered. With models ranging from dress models to sports models to professional dive watches, Citizen Eco-Drive runs continuously in any kind of natural or artificial light for a lifetime of use. Fueled by light, it never needs a battery.
Diamond cases and striking bracelet designs feature prominently in Elektra's exquisitely crafted styles.
Product Specifications
|Watch Information
|Brand, Seller, or Collection Name
|Model number
|Part Number
|Model Year
|Item Shape
|Dial window material type
|Display Type
|Fold-Over Push-Button Clasp with Safety
|Metal stamp
|Case material
|Stainless steel
|Case diameter
|26 millimeters
|Case Thickness
|Band Material
|Stainless steel
|Band length
|Women's Standard
|Band width
|14 millimeters
|Band Color
|Dial color
|blue mother of pearl
|Bezel material
|Stainless steel
|Bezel function
|Special features
|Water Resistant
|Water resistant depth
|330 Feet
|Warranty type
|Contact seller of record
Your low price
Can't find what you are looking for yet?
Just Click At My Favorite Online Store Under.
All you have to do is just check under the category of your product Or look for anything by typing in the search box to find A huge selection of your choices of any product, with amazing low prices many find extremely hard to find somewhere else! You'll absolutely be amazed by what you'll be saving here of time, efforts and money while shopping at ease, peace and quietness With people that care. So, go ahead and treat yourself.
If you need any further help please do not hesitate to write me something in the comment box below, or see my contact page.
Sam Ammouri


text:
Group Ex Schedule
Zumba combines high energy and motivating music with unique moves and combinations that allow participants to dance away their worries. Based on the principle that a workout should be fun and easy to do, Zumba is a "feel-happy" workout that is great for both the body and the mind.
- Location
- Studio I
- Instructor
- Charmaine


text:
Gregory family
Contributed on 10/29/17 by Jannagregory
Email This Contributor
Record #: 243974
On this site you can upload gravestone photos, locate ancestors and perform genealogy research. If you have a relative buried in Ohio, we encourage you to upload a digital image using our Submit a Photo page. Contributing to this genealogy archive helps family historians and genealogy researchers locate their relatives and complete their family tree.
Submitted: 10/29/17 • Approved: 10/30/17 • Last Updated: 11/2/17 • R243974-G243972-S3