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The music that was produced by Negro Christianity was the result of diverse influences. First of all, there was that music which issued from pure African ritual sources and which was changed to fit the new religion—just as the ring shouts were transformed from pure African religious dances to pseudo-Christian religious observances, or the Dahomey river cult ceremonies were incorporated into the baptism ceremony. Early observers also pointed out that a great many of the first Negro Christian religious songs had been taken almost untouched from the great body of African religious music. This was especially true of the melodies of certain Black Christian spirituals that could also be heard in some parts of Africa.
Maude Cuney-Hare, in book Negro Musicians and Their Music, cites the experience of a Bishop Fisher of Calcutta who traveled to Central Africa: "... in Rhodesia* he had heard natives sing a melody so closely resembling Swing Low, Sweet Chariot that he felt that he had found it in its original form: moreover, the region near the great Victoria Falls have a custom from which the song arose. When one of their chiefs, in the old days, was about to die, he was placed in a great canoe together with trappings that marked his rank, and food for his journey. The canoe was set afloat in midstream headed toward the great Falls and the vast column of mist that rises for them. Meanwhile the tribe on the shore would sing its chant of farewell. The legend is that on one occasion the king was seen to rise in his canoe at the very brink of the Falls and enter a chariot that, descending from the mists, bore him aloft. This incident gave rise to the words 'Swing Low, Sweet Chariot,' and the song, brought to America by African slaves long ago, became anglicized and modified by their Christian faith." **
* Rhodesia is the former name of Zimbabwe.
** Op. cit., 69,


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Providers take trip to the top with LeadingAge PEAK leadership summitApril 20, 2012
LeadingAge will host its spring annual meeting Sunday through Wednesday in Washington. Typically considered a "Capitol Hill fly-in" during which providers meet with their respective states' lawmakers to push the LeadingAge agenda, the meeting also will feature keynote speakers, workshops and educational sessions throughout. There also will be an exhibit hall Monday morning. Educator and a co-author of the best-selling "Switch: How to Change Things When Change is Hard" Dan Heath will deliver the keynote leadership address Monday morning.

subset: commoncrawl


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Ministry of Railways, Government of India, took to social media network Twitter today to highlight some of its Rail Budget points that would be taken under Swachh Rail Swachh Bharat programme.
In a graphic uploaded by the ministry, it highlighted that 17,000 bio-toilets in trains and additional toilets at 475 stations will be provided before the close of this financial year for the convenience of train-takers. Besides, it is alsogenerating more than 65,000 additional berths, installing 2,500 water vending machines; introducing 'Mahamana Express' with modern refurbished coaches.
It also has underlined that the Indian Railways has developed world's first Bi-Vacuum toilet which is being used in Dibrugarh Rajdhani Express. Under Make in India programme, it is proposed to increase the procurement of train sets by 30 per cent.


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Trial data: 5 ways to cut through the clutter
Experts often possess more data than judgment.
- Colin Powell
Your promotional materials are supported by data from clinical trials. Piles of numbers can intimidate even the brightest and most intrepid brand manager, so here are 5 ways to cut through the clutter and separate the wheat from the chaff.
1. Know the "trust hierarchy" of data
It's important to know that not all data sources are created equal in the eyes of the scientific and medical community. The clinical study report (CSR) is the Holy Grail of resources. It is the original and complete data source for a study, and has the most correct version of your data available.
A manuscript published in a peer-reviewed journal is the second most reliable source. Non-peer-reviewed publications and posters presented at congresses share the bronze medal in data reliability.
2. Have a close relationship with your peers in medical affairs or scientific communications
As a brand manager, you are not tasked with being your data's keeper. If one day you quickly need to obtain a study protocol, CSR, or publication, know whom to ask.
Managers in your company's medical affairs or scientific communications departments handle the numbers on a daily basis. Get to know them.
3. Statistical vs. clinical significance
Suppose a test on some of your data concludes that P<0.05, making the result statistically significant. Does this mean the result is important? Perhaps not.
A physician may see a small effect size and decide that the difference is simply insufficient to be relevant or influence clinical practice, no matter the P value. Don't just follow the statistics, do your homework to learn what your audience will believe.
4. Know what study endpoints mean to physicians
Your studies likely have several primary and secondary endpoints. Some may be germane to the way your target physician practices medicine, while others may simply indicate to a reviewer or the NIH that you've covered all your bases.
Does a physician care more about the way your drug influences a particular biomarker of the disease, or about the way your drug could improve a patient's quality of life?
Both measures can be endpoints in your study, but your brand's performance will reflect your understanding of which has greater clinical and/or emotional appeal. Conduct your research accordingly.
5. Ensure consistency and eliminate errors
It may seem a no-brainer, yet we are often surprised by mistakes and inconsistencies in the data provided to us by clients. To minimize embarrassing interactions with your med-legal review team and wasted time with your agency partners (worse yet, the possibility of errors creeping into promotional materials), start by putting the first suggestion on this list (knowing the trust hierarchy of data) into practice.
Use the most reliable data source available, preferably the CSR, and check everything against it. The fewer derivative materials comprise the supporting evidence for your brand, the more consistent and accurate the promotion will become.
Serve your brand and ensure accuracy by knowing where your data come from, what it mean, and to whom.>> Click here to return to Brand Incites blog page


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What happens when you find someone willing to donate a kidney to you – but it isn't a good match? Not too long ago, that would mean the search would need to continue until a suitable match was found. There is, however, an emerging strategy for overcoming this hurdle. It's called Paired Kidney Donation.
Using new software which takes into account many of the variables involved in a kidney transplant (blood type, HLA crossmatch, etc.), several institutions have been able to match three or more donor-recipient pairs.
Learn More »
As discussed in the Living Donation section of this site, living kidney donations traditionally provide better patient outcomes and increased kidney survival compared to deceased kidney donation.
Learn More »
There are a number of transplant centers who participate in regional exchange programs across the country.
Number of kidney transplants performed in the United States. Learn More


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Wednesday, September 16, 2009
Day 200....Miss You
Well the challenge today at 365 cards is to have an image that says "Miss You" ~ here is my take on the card!
Bazzil card stock, paper is Adorn It - Fern Collection (http://www.adornit.com/), cuttlebug, martha stewart loopy punch and my computer generated "miss you" to cover up my goofed up hand writing!
Thanks for stopping by ~ Happy Wednesday!
Posted by MicheleV at Wednesday, September 16, 2009
2 comments:
- Carolyn V said...
Aw! I love him! What a great card. Love the fun papers, too. :D
- September 16, 2009 at 6:32 PM
- Cardcrazy said...
Oh, that poor little puppy...so cute! Great card! ~~Pam
- September 17, 2009 at 7:28 AM
Subscribe to: Post Comments (Atom)


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Look at the following example: Here's a detailed step-by-step example that shows how this problem could play out: 1. Programmer P downloads the Konqueror web browser, receiving it under terms of GPL. 2. P learns of a new web standard that requires exercising a technique for parsing URLs that is patented by Corporation C. C has licensed the patent under an RF, non-exclusive license, but with a "field of use" restriction that says the license can be used to "implement the standard". The standard, as it turns out, covers only what browsers must do with URLs, and says nothing about the server side or clients that aren't user browsers. 3. P implements this technique in Konqueror, and seeks to redistribute the modified version on his website so that other users can benefit from Konqueror now complying with the standard. If he does, he is bound by the GPL under copyright law, because he is redistributing a modified version. 4. However, he knows full well of a condition on that code that contradicts the GPL (violating Section 7) -- namely, he knows that C's patent license prohibits folks from taking his URL parsing code and putting it into, say, a search engine. Therefore, under GPL Section 7, he is prohibited from redistribution. 5. You might think that he can simply assign his copyright to the existing copyright holder of Konqueror let distribution happen from that source. They could distribute under GPL, but they would be granting a self-contradicting license. Nothing (to my knowledge, but IANAL) prohibits someone from distributing copyrighted works under licenses that make no sense and are self-contradictory. However, it is certainly true that those who receive distribution of the works are stuck and can't undertake further distribution or modification themselves.Received on Friday, 6 December 2002 14:14:17 UTC
This archive was generated by hypermail 2.3.1 : Tuesday, 6 January 2015 21:06:48 UTC


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The Tentative Agreement and the Clerk Craft: Safeguarding Jobs, Creating New Opportunities
Rob Strunk
Clerk Division Director
(This article was first published in the April-June 2011 issue of The American Postal Worker magazine.)
Many provisions of the APWU's tentative agreement with the postal Service directly affect the Clerk Craft. Highlights of the entire agreement can be found on pages 6-9, but several Clerk Craft items deserve additional attention.
Automation and declining mail volume have had a dramatic impact on the craft, and we continue to lose jobs. Therefore, throughout negotiations, our top priority was to safeguard existing jobs and create new positions.
More Jobs
The Tentative Agreement returns to the APWU bargaining unit a minimum of 1,100 Call Center jobs that had been contracted out. The Call Centers will become part of the nearest installation, which will allow APWU members to bid on these positions. The number of positions could increase if the USPS can bring in Call Center work from other government agencies. Thirty percent of the Call Center jobs may be reserved for APWU-represented rehabilitation employees by seniority.
APWU President Cliff Guffey and the rest of the negotiating team also were determined to return to the Clerk Craft duties that have been gradually transferred to EAS employees. A minimum of 800 duty assignments will be created in the craft to perform administrative and technical work that is currently performed by EAS personnel.
Lead Clerk, PS-7, positions will be established in mail processing plants and customer service. A ratio of those employees was negotiated for both areas. The future of these positions is to return to craft employees work that has been performed by 204Bs. Therefore, the number of 204Bs will be reduced. They will be eliminated from offices with supervisors except to fill absences of 14 days or more and vacancies of 14 days or more, not to exceed 90 days. Employees serving as 204Bs also will be required to return to the bargaining unit for a pay period in order to bid and to avoid having their duty assignments reposted.
New Assignments and Bidding Opportunities
The union negotiated new rules that authorize management to post "nontraditional†duty assignments. The following are examples of non-traditional duty assignments that may be coming soon to a post office near you:
- Three 12-hour days
- Four 12-hour days, with eight hours built-in overtime
- Four 10-hours days
- Four 11-hour days
- Three 11-hour days and a seven-hour Day
- Five seven-hour days
- Five six-hour days
We also negotiated restrictions on these positions.
Percentages were set to prevent management from over-using these types of assignments. In addition, national APWU officers will review the staffing levels of all offices to address any abuses by local management.
No current full-time employees can be involuntarily assigned to a duty assignment of less than 40 hours per week or more than 44 hours per week. There will be no mandatory overtime in functional areas where non-traditional duty assignments are created. Furthermore, if the duty assignment is for less than eight hours a day, the employee will be paid out-of- schedule pay for hours worked outside of his or her schedule.
If the number of hours of work on these assignments is changed, the assignment must be re-posted.
All Part-Time Regular (PTR) assignments will be converted to fulltime assignments. In many areas where employees chose to become PTRs in lieu of being excessed, the decision was catastrophic because the employees' schedules were reduced to just four hours per week. Under the Tentative Agreement, the minimum number of hours worked by full-time regulars will be 30 per week.
In small offices, Postmaster Reliefs will be eliminated. In addition, dual-appointment Rural Carrier Associates (RCAs) will be wiped out, and Postal Operations Administrators (POAs) will be a thing of the past. This should bring more hours and more work for our members. The Tentative Agreement also restricts the amount of bargaining unit work that postmasters can perform in small offices, and PTFs have been eliminated in Level 21 offices and above.
Employees will be allowed unlimited bidding on jobs that do not require off-site training or a deferment period.
Other Gains
Regardless of our efforts to limit excessing, we cannot protect workers from all reassignments to other installations. We tried to lessen the impact of excessing by agreeing that affected clerks may elect to transfer within 100 miles or fill residual vacancies without the loss of seniority. Allowing this voluntary action will help to reduce the number of forced relocations.
For years, the Clerk Craft has attempted to eliminate "bid blocking.†Under the new provisions, if a senior bidder withdraws or fails to fill a vacancy, the opportunity will be passed to the next senior bidder. This process continues until the position is filled.
Relief and pool assignment employees can now cover vacancies, provided notice of the employee's schedule change is given by the Wednesday preceding the service week that the position is set to start. Relief employees can also be used to cover vacancies in other installations. This provision is primarily designed in the event the work is returned to the Clerk Craft in smaller installations.
The Tentative Agreement also creates a new type of position, the "Delivery/ Sales Services and Distribution Associate.†Employees in these assignments could be used in small offices to work the window, sort mail, and deliver to non-prescribed routes. We have been working to create this position for a long time in order to prevent non-bargaining unit employees from doing these duties, which our members are capable of.
We also agreed to discuss with the Postal Service changes to Article 37 that would require all future excessing in the Clerk Craft to be done by seniority, regardless of level. When the old excessing rules were written, we didn't contemplate the changes we currently face, so this is an effort to "right a wrong.â€
While these are some of the main provisions that will impact Clerk Craft employees, there are other important issues included in the agreement. All members are encouraged to study the Tentative Agreement, which can be found at www.apwu.org.
As Clerk Craft director, I want to thank all of the Assistant Directors — Pat Williams, Lyle Krueth, and Lamont Brooks — for their hard work. I want to also thank our many National Business Agents who contributed to the process. I'm proud of the efforts of Clerk Craft officers and of the union's full negotiating team.
During negotiations, the APWU didn't just think outside of the box — we broke down the sides of the box to reach an agreement that benefits both parties.


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By: Alan Cunningham(Author), Kevin Mills(Editor)
98 pages, colour photos, colour & b/w illustrations
A complete transcription of the journal of botanist Allan Cunningham while visiting Norfolk Island in 1830, with modern commentary by the author. Allan Cunningham is known widely for his exploration of inland New South Wales, however, in 1830, his botanical investigations took him to the convict settlement of Norfolk Island. A keen observer, Cunningham documents his visit to Norfolk Island in his journal, published here in full for the first time. Described are not only the plants of the island but also the day to day activities of life on the island during the "second convict settlement".
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For Non-Catholic Families
De Smet Jesuit is a welcoming community where students quickly fit in and find their place – whether they come from a Catholic grade school, another religious school, or a public school.
There are a number of students currently at De Smet Jesuit, plus many graduates, who are not Catholic. In some cases these students and their families came to our school for its attributes other than faith formation, including our top-tier academic program, co-curriculars such as athletics, the school's rewarding service program, and an environment where students, teachers and staff take high school seriously. Additionally, others have said they appreciated De Smet Jesuit simply because students are allowed to pray at school.
Our new students quickly make friends a number of different ways: Odyssey Week, a week-long event for freshmen that promotes teamwork with an emphasis on fun; our Companeros program, under which freshmen are paired with a member of the senior class who can show them the ropes; plus more than 50 student-run clubs and 53 sports teams.
The admissions process (application forms and deadline; access to the applicants' grade school records, etc.) is slightly different for applicants coming from a non-Catholic school. For specific details please contact our Admissions Office at 314-567-3500, ext. 1227.


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IT WOULD have been a textbook response like any other day – but this was not an ordinary day.
It was Christmas Day in 2011 and paramedic Brian Bridges and his colleagues were responding to reports of a car crash on the New England Highway South of Tamworth.
When they arrived they found the car had crashed into a tree and the young male driver had died at the scene.
On the back car seat were Christmas presents to be given to his loved ones.
"It was clearly a picture that was painted in our memory for the rest of the day," Mr Bridges, a veteran paramedic of more than 30 years, said.
"Unfortunately for that family they were, instead of unwrapping presents in a happy and enjoyable environment, they were grieving and planning for the funeral after someone's death."
In a message of road safety to residents on country roads, particularly in the holiday season, Mr Bridges urged motorists not to take any risks.
He said paramedics in country areas were not immune to the trauma seen on country roads and it was more common in peak periods.
It was a time he said that paramedics can "dread" because of the inevitable call outs to road trips gone wrong.
"We are often called out to some nasty accidents over the holiday period," he said.
"Some accidents have been fatal in the past.
"It is a time that we don't look forward to, in terms of working."
The experienced paramedic said, sadly, many of the accidents seen by paramedics on our roads are preventable.
"We just ask that travelers be very mindful and vigilant during their travels," Mr Bridges told The Leader.
"A lot of accidents occur on country roads and a lot of them are fatalities and they do occur on bends.
"We urged travelers to be mindful and to be well prepared.
"Plan your travel and take regular breaks. Drive to the conditions, day and night because fatigue can strike at any time.
"If the traffic becomes somewhat bottlenecked, it might be time to take a bit of a break and have a spell.
"We want you to stay off the phones, no calls or texting.
"Of course no drink driving.
"The most precious presents that you are carrying at Christmas, in that car, are you and your family."
Mr Bridges said some accidents can also caused by two things – being unfamiliar with the road and also a little too familiar and becoming complacent.
"If you're not familiar, take a break," he said.
"If there's a couple of drivers, rotate them, rest breaks are enough, particularly with children on board.
"(And) be mindful of wildlife on country roads, particularly at night time."


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S: Happy Father's Day Papa!!
BC: Made just for you by "Punkin". Father's Day 2011.
1: Since Day 1 you have been one of my biggest supporters. I have always admired your strength and how loved and admired you are by everyone around you. I could not have handpicked a better grandfather nor would I have wanted to. I am and will always be your "punkin".
3: My Little Girl "Gotta hold on easy as I let you go Gonna tell you how much I love you Though you think you already know I remember I thought you looked like an angel wrapped in pink so soft and warm You've had me wrapped around your finger since the day you were born You're beautiful baby from the outside in Chase your dreams but always know the road that'll lead you home again Go on, take on this whole world But to me you know you'll always be, my little girl." - Tim Mcgraw
4: "Grandfathers are just antique little boys"
5: Even Vixen loves her "Gwandaddy"
6: "My role model was my grandfather. He instilled in me the feeling that no matter how successful you are you have a responsibility to help others." - Kevin Johnson
7: "A grandpa is someone you never outgrow your need for" - Anonymous
8: "Being grandparents sufficiently removes us from the responsibilities so that we can be friends" - Allan Frome
9: "You are my sunshine..."
11: Papa and his girls!!
13: Papa, On this Father's Day, no words could possibly express how much your love and support has meant to me over the years. From sitting through 15 years of dance recitals, countless pageants, and hundreds of sports games, you were always there to support me and cheer me on. I never have had a great relationship with my dad, but in the end that's never mattered much to me. Why should it when I have someone like you? You've always been there for me no matter what. Although I'm nearly grown and don't quite fit in your lap like I did when I was a little girl, I will always be your "punkin" and you will always mean the world to me. I only hope that I can make you proud! I love you forever and for always and I hope that you have an excellent Father's Day. You deserve it!! Love, Punkin


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Tom Coughlin isn't worried about defensive coordinator Steve Spagnuolo's job opportunities getting in the way of the Giants' Super Bowl run.
Spagnuolo's name has been mentioned in connection with many of the head-coaching vacancies around the league, but Coughlin said he believes Spagnuolo is focused on one thing this week – shutting down the Eagles.
"No, it won't be a distraction," Coughlin said. "I have talked to him. He has tried to keep me posted but there isn't any doubt in my mind that his focus is right here preparing for the Philadelphia Eagles."
Spagnuolo interviewed with the Jets on Saturday and is considered one of the top candidates for the position that was opened when Gang Green canned Eric Mangini a week ago. Spagnuolo has also had conversations with the Lions, Browns and Broncos.
"Last week was a League-wide opportunity for anybody who was in the bye week and a couple of days were set aside for this according to those rules, Couhghlin said. "And they did not interfere with anything that we are doing here. And he, as always, is very much focused on the job at hand."
Spagnuolo was offered the Redskins head-coaching job last year, but turned it down after the Giants made him the highest paid defensive coordinator in the NFL.
"What's different? That was the case last year," DE Justin Tuck said last week. "Our focus is these playoffs and I'm sure his is, too. We'll handle that when the time permits it I guess."
Spagnuolo's defense led the Super Bowl charge last year for the Giants. In a Divisional Round win over the Cowboys, DE Michael Strahan and company harassed Dallas QB Tony Romo forcing him into erratic throws. And in the Super Bowl the Giants' defense did what most considered impossible – slowing down Tom Brady and the Patriots' offensive juggernaut in a 17-14 Super Bowl win.
"It says enough by saying we want him back. I think that says enough," Tuck said. "Obviously the numbers and success speaks for itself. I don't think we won a Super Bowl before he got here. I feel as though he has been a huge part of the success we've had – not only defensively but this entire team is appreciative with the way he goes around here and goes about his business. When the question comes 'Do we want him back,' obviously."


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Or download our app "Guided Lessons by Education.com" on your device's app store.
Make a Hovercraft!
Here's a fun activity that will introduce your fourth grader to the concept of frictionless motion by constructing a hovercraft. A hovercraft is a vehicle that travels on a layer of compressed air just above any kind of land or water surface. The compressed air serves as an invisible cushion that eliminates almost all friction between the vehicle and the surfaces.
What You Need:
- Piece of cardboard
- Paper hole punch
- White glue
- Balloon
- Pair of scissors
- Ruler
- Small spool
- Sheet of paper
What You Do:
- Cut a 4-inch square out of the cardboard.
- Punch a hole in the center of the cardboard. The hole should be the same size as the hole in the spool. If using a spool with yarn or string wrapped around it remove the string.
- Glue the spool to the cardboard on top of the hole. Make sure the holes line up. Use enough glue to assure that no air can escape between the spool and the piece of cardboard.
- Cover the top of the spool with a circle of paper. Glue it to the spool and allow to dry.
- Punch a hole in the middle of the paper cover where the hole of the spool is. Now your hole should run through the paper, spool, and cardboard without any obstructions.
- Inflate the balloon and pinch the end to keep the air from escaping. Stretch the balloon over the top of the spool.
- Set the hovercraft on a level surface (table top, floor, etc) and let go of the balloon. Give the hovercraft a few gently pushes.
The air flowing from the balloon through the holes forms an air cushion between the craft and the flat surface, which reduces the force of friction (the resistance that occurs when two object rub against each other). With little friction to slow it down the hovercraft shoots across the table!


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Silverdale Events Update is a FREE digest of events in the village and surrounding area, delivered directly to your inbox each Wednesday.
Every week you'll receive a listing with details of events in and around Silverdale for the following two weeks. Also in the e-mail there are links to appropriate websites for charities and organizations, performers and film reviews. Links are also posted to Parish Council agendas and minutes, plus any other relevant information.
To receive your free copy every week please click on the subscribe button at the top of this page and fill out the registration form there.
This site is currently being re-developed, but here you will be able to find diaries of events in the village and surrounding area, a directory of local businesses and services, links to the websites of local charities, organizations, businesses and individuals, plus an online forum where you can have your own say. If you have any feedback regarding the site please post it on the forum pages.


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2 days ago I was hit by a sudden need to have Nasi Lemak for lunch. It got me thinking how nice it would be to know how to make it myself. As long as I have the ingredients, I could make it at any time (oh-so-dangerous for my waistline!). And so I scoured the Internet on how to make Nasi Lemak with the help of my trusty sidekick, Mr. Google. At last, I found 2 versions that I felt was easy enough to follow.
The result?
Tadaaa!!!! I cooked everything myself except for the fried peanuts. My dad did that while I was a work.
I am so happy it worked out well! The rice was fragrant and tasty even though I just used instant coconut milk. The sambal wasn't bad either but I had to add some sugar to give some dimension to it's taste. My dad even gave his stamp of approval! I am feeling very grown up and proud of myself at the moment. Lol!
There's just something so satisfying about eating something you created yourself, especially if you thought that it was way too difficult all this while.
The best part of knowing how to do this is that I could customize it according to my liking. Mau lebih sambal kah, lebih bilis kah, I'm in control. Best kan? Since I find that sambal needs some work I won't be posting the recipe today. Will post it up once I've found my 'magic blend' so to speak.
That's all for now dearies... What's your favorite food? Have you learnt how to make it?


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Social media websites are an important part of many people's everyday lives. They log on first thing in the morning and check back frequently throughout each day. How are you able to start out marketing to these individuals? Read this article to learn more about social media marketing.
Try and add exclusive content to entice people to follow you on various social media sites. When people see deals or promotions on Facebook they will want to spread the word about what your site has to offer.
Respond to questions or comments posted by visitors. You'll want to stay very active and constantly check to see if anyone has sent you a comment. You can also adjust your settings so that you receive an email notification when content is posted. Keep in mind that anything you post becomes public knowledge.
Make sure your advertisements are placed in a place on your profile where people will see them. You need to experiment with this to see which areas work best, but it's worth the time spent!
Social media is a great way to draw in new customers. Tell your customers about your current promotions, or new locations. Have them print coupons and give them access to limited offers reserved to your Facebook friends. Give people a reason to believe that becoming your friend on Facebook will be a good thing for them.
There is data suggesting that people tend to read marketing messages on social media sites during business hours on weekdays. Utilize tools that allow you to write your posts anytime you want to, but they are uploaded and published during hours that you choose, including those when most people are actively online.
You should always follow your followers on your Twitter page. Most Twitter users expect this courtesy. It will show that you have respect for your visitor and they are equal to you. Remember to send a tweet to any new followers; if they see that you are following them, they will be more likely to continue to follow you.
Make sure to proofread anything you plan on submitting on Facebook or Twitter. You will need all of your content to appear professional. Use abbreviations with care on Twitter. Some are alright to use. Try your hardest to create good looking content.
When individuals decide to follow you on social media sites like Twitter, follow back. Your customers should feel that your business is a partner, not an elite. Individuals desire to know that the business they will be dealing with cares about their consumers. Following users who follow you is a simple, effective gesture that boosts your esteem among customers.
Be an active member of social communities and post often. Social media has everything to do with being social, interacting, and being seen. Blog about something that generally interests you to get to talking. Doing this will quickly increase the number of social connections you make.
It's not just enough that you're adding content to your Facebook page; the content you add needs to be interactive and entertaining. You can post YouTube videos, articles, or other fun links that are related to your product. Keep the ratio of serious and relevant content relatively equal.
Add some competitiveness to your Facebook page. People love contests and competitions. Have one for your Facebook page; you will see momentum in "likes". Before you know it, you will have a bunch of new "likes" on your page.
Create irresistible headlines. Often, the headline is the only thing that users on a social media site see. That means that you are almost fully dependent on the magnetic pull of the headline to capture the reader's attention. Depending on your headline, it can either make you or break you.
Make it easier for readers to subscribe to you so that they can easily get informational emails. Emails can remind your readers of new content on your social marketing sites.
Check out your competition, and see how they are taking advantage of the realm of social media. Reevaluate your social media marketing campaign to eliminate unsuccessful methods. Once you do, take advantage of the things you've learned, and use them to take your business to new heights.
The people who visit social networking sites daily can become your viewers. If you intelligently apply the social media marketing advice contained in freedom cash system scam this article, you should see your business improve. Very soon, you will see the results you want!


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You're likely to see many more pictures at the ball field this summer! This is my little man watching my brother play baseball last night. My brother plays for his HS team and then a few different traveling teams during the summer and fall.
For more A Thousand Words Thursday, check out Cheaper Than Therapy!


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London: Singer Shakira has reportedly donated $400,900 to rebuild a school damaged in an earthquake in Haiti.
The "Hips Don`t Lie" hitmaker had pledged to rebuild the Elie Dubois school in Port-au-Prince shortly after the disaster hit the country in January 2010. Her Barefoot Foundation will team up with the Architecture for Humanity charity in the endeavour.
"I`m convinced the key to achieve a dignified future for the Haitian people is through education," dailystar.co.uk quoted her as saying.
"I`d like to say to the girls that this is an enormous opportunity that life is offering you - education. So make the most of it because I`m sure you can achieve any of your dreams," she said.
Shakira visited Haiti earlier this week and announced a deal with the Inter-American Development Bank, which will match her donation to rebuild the education facility.


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Previous entry:
« This is either a poem or a collection of thoughts.
Next entry:
Unconscious Mutterings »
Uh oh, here comes the Walter
Thanks to my old chum Mr B------, I have been endowed with a moniker that evokes great poetical talent. Playing upon my last name and my fondness for a certain song that is popular on the radio, he has decided to start calling me Nitty. I am only too proud to be connected with the genius that brought us such beautiful verse as:
We're standin face to face, uh
I knew right where to take her
That private room called the bathroom
Uh oh, here comes the waiter
I am so amused that rappers are now sampling The Archies. What's next? Yummy Yummy Yummy I Got Love In My Tummy? Puppy Love? The future is so bright!
srah - Saturday, 16 October 2004 - 9:41 PM
Tags: music
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- Home
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Mid-Year Dues Reduction! Apply for membership now and join the great networking and programs with more than 500 women in DC CRE. Active members (5+ years in CRE) are now $265 and Associate Members reduced to $225.
Any questions regarding general membership should be directed to Marianne Wilson or Amanda Ryland.
Questions regarding student membership should be directed to Auja Little at email@example.com, or visit our student specific website at www.ucrewdc.com.
Click on any of the headings below to learn more about CREW DC membership.
Active – Employed in the field of Commercial Real Estate for at least five years.
Associate – Employed in the field of Commercial Real Estate for fewer than five years. Number of associate memberships available is limited.
Student - A student in an accredited degree program leading to an undergraduate or graduate degree in Commercial Real Estate or a related area (i.e. architecture, urban planning, business, finance, etc.)..
Public Sector – Active member who shall be employed full-time in any federal, state or local government capacity or in an economic development entity reasonably recognized by the Board of Directors. Such Public Sector Member shall have all of the privileges and rights of Active Members.
Retired – Active member of the Association or another CREW chapter for a period of at least two years prior to such member's retirement from fulltime or part-time employment, and shall have been actively employed in the field of Commercial Real Estate for at least five years.
Submit a completed CREW DC membership application form to Membership Chair Melanie Stehmer-Townsend at email@example.com.
Returning or transferring members should contact Melanie for reinstatement/transferring requirements.
Acceptance of new members is dependent on the availability of membership openings. Membership shall be comprised of the following categories or disciplines within the field of Commercial Real Estate:
Design – includes architecture, engineering, interior design, space planning, and environmental assessment.
Build – includes development, construction (general contractor), and construction management.
Own – includes acquisitions, investments, corporate real estate, and non-profit real estate.
Management – includes asset management, property management, and facility management.
Broker – includes leasing, sales, brokerage, landlord representation, and tenant representation.
Finance – includes finance, mortgage brokerage or banking, institutional lending, accounting, appraisal, and title and escrow.
Law – includes real estate law, land use and zoning, and other transactional law.
Promotion – includes advertising, business development, marketing, research, and media organizations.
Public Sector – includes federal, state, and local governments, and economic development entities.
Student – includes any person currently enrolled in an accredited degree program leading to an undergraduate or graduate degree in the Field of Commercial Real Estate as hereinafter defined
Vendors – includes any person providing services to the commercial real estate industry but not employed by a commercial real estate organization.
Other – includes consulting, human resources, IT, and other roles employed in a commercial real estate organization.
General Membership: Click here to access the online CREW DC Application form. Direct any questions to Marianne Wilson or Amanda Ryland.
Student Membership: Complete a Student Member Application. Direct any questions to Auja Little at email@example.com.
Please remember to set up your professional profile in CREWBiz so you are included in keyword searches done by other members looking to give you business!
Annual Membership Dues cover the 12 month period from January 1 - December 31. New members admitted after July will be billed on a half-year basis (excluding students).
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In addition, there is a non-refundable $25.00 application fee for prospective members. This application fee does not apply to transfers from other CREW chapters.
For existing members (excluding student members), click here to log in to CREWBiz (click "retrieve password" if you don't remember your password) and either renew online with a credit card or print your invoice to mail in with a check. Please contact CREW Network with any questions at 785-832-1808 or email@example.com.
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Membership expires on December 31st of the current year. If dues payment is not made by February 14th, benefits of membership (i.e. program attendance at member rate and inclusion in the CREW Network directory) will not be available.
Annual Membership Dues and the non-refundable application fee are due upon submission of the application. If a prospective member is not
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If you believe you qualify for the Member Emeritus or Retired member classes please contact the Membership Chair.
Current CREW members from other chapters who would like to transfer to the Washington, DC chapter should contact the Membership Chair to
discuss the specifics of their request.
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Ways to Help the NFB of Kentucky
NFB of Kentucky State Convention
The National Federation of the Blind of Kentucky (NFBK) holds a state convention annually. Members from across the state will be present to discuss the issues that affect the blind of our Commonwealth. NFBK is the state affiliate of the National Federation of the Blind (NFB). Our National center is located in Baltimore, Maryland. We are the largest organization of the blind in the nation with a membership of over 50,000. We are the blind speaking for the blind.
In addition to conducting the business of the organization we will plan social activities for our members. We are hoping that you will be able to help make our convention an even bigger success by donating an item that can be used for a door prize. Door prizes have always been a popular attraction at our conventions. The NFB of Kentucky is a 501 (c) (3) not-for-profit organization.
If you would like to make a donation, please donate using our Go Fund Me initiative.
The NFB of Kentucky will be selling key chains. Contact a chapter president if you are interested in purchasing one or more.
They are round and silver in color. NFB is written in bold letters on one side and the retro WHOZIT NFB logo is on the reverse side. They are well-made and only cost $3.
The National Federation of the Blind has been around for 77 years, 9 months, and 2 days.© 2009-2018 National Federation of the Blind All Rights Reserved


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|For the 1st time in more than a year, Emalia joined Zadoc on a board!
|Emalia, Kahala, and Hayden Brown
|at the Miracle Pool!
|Two Emalia's!
|Partners in crime for Girls' Night Out (last posting): Becky and Jorma Palmer (If Can Entertainment)
|Becky and Jane at Hayden's 11th birthday party: a shave ice night at Tobi's (Fisher) Shave Ice in Paia
|Emalia and her dad at #24
|The honi monster loves his mama!


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Happy Mother's Day to the wonderful mom's that have shared their children with me this year in my First Grade class! We have had a wonderful year and I can't believe I am about to send my sweet students off to Second Grade!
For Mother's Day, the students created a self-portrait of their mom. They had SO much fun creating these and loved when I gave out the "diamond" earrings and "pearl" necklaces! On the bottom of their self-portrait each student wrote why they love their mom.
Happy Mother's Day to my Mother and my Nana!


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ABOUT OTAN TRAINING: Workshop Descriptions
|Workshop title
|Easy to Make Quizzes and Flashcards for Tablets and Mobile Phones Part 2
|Workshop description
Harness the power of the smartphones and tablets that more and more of your students have as a free student response system, or as a 24/7 flashcard delivery platform for flashcards you create for them. Participants will learn how to use Kahoot to create competitive and exciting quizzes that your students respond to using their smartphones and tablets. (They can use classroom laptops and desktops to answer as well.) Participants will learn how to use Quizlet to create flashcards for study and practice. For both Kahoot and Quizlet you use their respective Web sites to create the quizzes and flashcards, but students can access them from their smartphones and tablets! Part 1 focuses on Kahoot. Part 2 focuses on Quizlet.)
Prerequisites: Participants should be comfortable using a computer and the Internet and know how to download and install an app to a smartphone.
|Session length
|Workshops: Synchronous online trainings, or series of trainings, in which facilitator and participants interact by voice or chat.
To find out more about any of these workshops, including dates and times offered, please check the California Adult Education Professional Development Web site. This site has information on OTAN offered training, as well as training offered by CALPRO and CASAS.
Minimum System Requirements for Participating in Online Workshops:
- Broadband Internet connection (DSL, Cable modem or faster, no dial-up).
- Computer with speakers or headphones (some classes require microphones too).
- Active e-mail account through registration for the course as an OTAN member.
- Some workshops have other special requirements, please read the workshop descriptions carefully.


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SRC Arena/Event Center at Onondaga Community College
Sunday, January 7th, 2018 11:00am – 3:00pm
Enjoy the FREE show at this premier event with your friends, fiancé and family. Visit with the finest wedding specialists who are here to help plan your perfect day. Check out all the latest wedding fashions. Fashion Shows throughout the day.
Save hundreds of dollars on your wedding with SHOW ONLY SPECIALS! Win free prizes and free giveaways.
Come to the show and have an opportunity to win one of our Ultimate Bridal Prize Packs!
Register For Free!
TICKETS ARE FREE, but registering in advance is highly recommended. Fill out the form below to register for the show and your ticket will be emailed to the bride's email address as given below.
For more information call 1-800-724-1800


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It should be weird that my youngest cousin is graduating from high school and is off to college, but it's not. And that's kind of weird in itself.
Her graduation party was Saturday, so we spent a sunny afternoon with the relatives and watching my family play cornhole. The real winner there was me, who acknowledges I'm terrible at cornhole, refuses to give in to the peer pressure, and has more fun socializing instead.
We were set to go watch the Pens game at my parents' house in the evening, so since we were gonna be in the area, we decided to make a trip to visit Paul's family, too–mostly the new cat, Link, who was a good time. Strangely, although I've often been critical of how they used to treat their dog before it died, I think a cat could be really good for them. They're more independent and easier to take care of, which I think is what they need.
Unfortunately, back at my parents' place later, the Pens lost. The series is now tied.
On Sunday, we finally saw Get Out–Row House was doing a Creepy Communities theme, so even though I thought we missed it in theaters, we got a second chance. I was kinda sorta but not really spoiled, unfortunately, thanks to a headline about similar themes, but at least it wasn't a full spoiler. But while it didn't spoil the plot itself, it did spoil the experience, which is almost as bad. Because I had an idea of what direction things were going, I kept thinking about that and trying to see how things fit in with it, rather than just enjoying it and watching things unfold, which was kind of frustrating. But we really liked it and thought it was really well-done.


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Individual Artist Arts Partnership Grants Sponsorship
For individual artists, $500 can mean the difference between pursuing another step in their artistic career or not. With a small amount of money, artists can put up a show, buy a piece of equipment, purchase supplies, take a class, stage a small production, etc.
Until 2012, The Arts Partnership was not in a position to grant money to individual artists; the money we re-grant from the tri-cities is designated only for non-profits, and we had not had an available pool of funding for other granting. Fortunately, we were able to pull some money from the sale of the CSA shares and funnel it into this new granting program. We have thus far granted 10 individual artists $500 grants each. Their applications included:
- Taking a dance teaching course
- Continuing an advanced calligraphy course
- Sending a play off to a dramaturgist for critical edits
- Traveling to Michigan to interview a pivotal character for a documentary
- Traveling to Scandinavia to do research for an art project
- Going to NYC to take advanced dramatic movement classes
- Funding an art teachers art showcase opening event
- Facilitating an art project around Alzheimer's patients
Our hope moving forward is to grow the granting pool of dollars for this program. We are asking you to consider supporting 1 Individual Artist Arts Partnership (IAAP) grant. Our goal is to double the annual amount to $5,000, or 10 grants.
If you agree to fund one IAAP grant, you may
- Elect to have it named after you: the Individual Artist (your name/business) Arts Partnership grant –or-
- Put your funding into the general pool of dollars already raised
Either way, unless you request to remain anonymous, your name will be included as one of the funders of this overall program.
We believe that with additional support like this, individual artists will see a significant benefit to staying in our communities, continuing their artistic endeavors, and contributing to the overall quality of life that benefits us all.


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Maru is a tiny little gem on Burnet near 45th. They serve sushi, traditional bento boxes, and various other delicious Japanese entrees. The food was great. The service was... interesting. Our server was uber-attentive on the verge of stalker-like. She was just kind of always looming, but didn't really do anything. However, it was comical enough that we had a good time. Plus, they gave us a complimentary dessert, which (you'll see in the video) also proved to be... interesting. Enjoy!
4636 Burnet Road, Austin TX


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Louis Vuitton
New Louis Vuitton Saint Jacques Epi Leather PM On Sales Online
PRISTINE Merchandise is in "like new" condition. There are no signs of wear or any other visible imperfections. EXCELLENT Merchandise is in exceptionally good condition with very little wear. Any imperfections are described in detail. VERY GOOD: Merchandise is in very good condition for its age. Older items may exhibit very light patina and wear relative to the age of the product, and more contemporary items will be just short of "excellent". GOOD: Merchandise is in good condition and typically will appear mildly to moderately used. There may be some surface scratches and/or other imperfections. Older items may exhibit patina and wear related to their age. Please review the item listing for specific details regarding condition.
This New Louis Vuitton Saint Jacques Epi Leather PM On Sales Online is refined and elegant. Crafted from Louis Vuitton's signature sturdy black epi leather and sturdy base, the bag features short straps, subtle LV logo design at the front and an overhanging brass-tone zip closure. The interior is made with gray suede alcantara and showcases a slip pocket. Authenticity code reads: VI0975. **Note: Shoe photographed is used as a sizing reference, and does not come with the bag. These are professional pictures of the actual bag offered by us.
Estimated Retail: $1,020
Condition: Great. Tarnished zipper pull and minimal wear in interior.
Accessories: None.
Measurements: Handle Drop 5.5", Height 9.5", Width 9-15", Depth 4"
Designer: Louis Vuitton
Model: Saint Jacques Epi Leather PM
Item Number: 1747/15
Hardware: brass-tone
Color: Black
Handle Drop (in inches): 5.5
Height (in inches): 9.5
Width (in inches): 9
Depth (in inches): 4
Authenticity Details: VI0975
Primary Material: Epi Leather
All our items are shipped from the US. We use FEDEX within the US. For international shipping please note that the customer is responsible for any import duties, goods and services tax (GST), value added tax (VAT), and customs fees.
No Returns- Final Sale. Items with exotic skins are not available for shipment outside the US. Please contact Customer Care for further questions.
Very Good used


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How hard is it to find an apartment in Copenhagen?
Hi from California!
I am wondering how hard it *really is* to find a flat somewhere in Copenhagen? When I talk to certain people, they say it's really tough and many people in Denmark are on a waiting list for practically their whole lives. But then I talk to other people and they say it's possible.
I am looking to relocate to Copenhagen whenever I figure out... how to go about finding a flat. I don't want to spend months in a hotel while I am looking. It would be nice if I could find a place before I even go, but I understand that is not likely.
About me:
Dutch Citizen, so I don't need a Visa
Self Employed so I don't need to find work
I can afford to pay 9,000kr. (or even more), but I'd rather not if I don't have to :)
I don't need much. Just a basic studio near the train so I can run around when I feel like it.
If anybody can give me any specific advice, I would appreciate it! Thank you!


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Orchestral Studies Diploma:
Advanced Eastman School of Music students of violin, viola, violoncello, or double bass have the opportunity to perform with the RPO on classical subscription concerts. Learn more about OSD and how to apply here.
Arts Leadership Program Internships:
Opportunities for Eastman School of Music students to work alongside professional staff at the RPO's offices during their senior year. Learn more about ALP and how to apply here.
College Program:
Music appreciation class, lecture demonstrations and full orchestra concerts at local colleges. For more information, contact Barbara Brown, Director of Education, at email@example.com.


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Harry Styles served up a treat for Cindy Crawford's kids over the weekend (07-08Sep13) by making pizzas with them after striking up an unlikely friendship.
The One Direction hunk recently met the supermodel's son, Presley, through mutual pals and he stopped by his new friend's Southern California home on Sunday (08Sep13) to cheer the teen up as he recovered from surgery on his tonsils.
The 14 year old later posted a Twitter.com photo of Styles and his friends posing in the kitchen, alongside the caption, "Thanks for stopping by the house again and helping the boys and I make our pizzas @harrystyles."
Responding to the message, Styles, who worked in a bakery before shooting to fame, tweeted back, "Anytime brother. See you for surfing soon."
Crawford's 12-year-old daughter Kaia also took part in the food fun, and shared her own snaps with the What Makes You Beautiful singer on her Instagram.com page.
Styles, 19, was previously spotted at Crawford's home late last month (Aug13).


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This information is provided for your convenience and may no longer be valid.
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Be the first OregonLive.com user to add photos or videos of Electronic Tracking Systems
Since 2006, Electronic Tracking Syste has been providing Electronic Parts And Equipment from Salem.
Electronic Tracking Systems is located at 875 Madison St NE, Salem, OR. This location is in the Highland neighborhood. This business specializes in Electronics.


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Dr. Nutan P. Malpathak
Teaching experience: 22 years
Joined as a lecturer in the Dept. of Botany,
University of Pune, in 1992.
Appointed as selected Reader in the Dept. of Botany, University of Pune on temporary basis 2000-2002.
Appointed as regular Reader through CAS from 16th March 2002
Course in charge for courses: Cell Biology,
Teaching other courses Plant Biotechnology and Bioinformatics,
Plant Biodiversity
1. In Vitro Cell Dev. Biol.-Plant 41:291-295. (2005)
2. Plant Cell Tissue Organ Culture 80:247-257 (2005)
3. J. Plant Biol. 31(2):95-99 (2005)
4. Current Science 87(10): 1442-1447(2004)
PCR of A4 and LBA 9402 (pBIN 19) mediated primary and secondary hairy root clones for rol A.
Patent granted entitled "Process of extraction of solasodine" ref no: 955/MUM/Y/310304 dt 15.9.03
Genetic Transformation in Solanum khasianum
Patent filed entitled" process for enhancing production of furanocoumarines using tailored cell cultures (2500/MUM/2008, dt 28/11/08)
New Biotechnology 25(1):85-91 (2008)
Bioorganic & Medicinal Chemistry 17:7052-7055 (2009)
In Vitro Cellular and Dev Biol. –Plant 46:108-116 (2010)
Appl Biochem Biotechnol 163:756–764 (2011)
Bioremediation, Biodiversity and Bioavailability 5(1): 1-9 (2011)
Journal of Botany (2012) (in press)
Transformation with LBA 4404 harboring pCAMBIA 2301
Establishment of lines: Cell Cultures & Multiple Shoot cultures, Histochemical localization
Exploring Ruta graveolens L cultures for enhanced production of furanocoumarins
Modification of non-phytopathogenic bacteria to mediate gene transfer to diverse plants
Non-Agrobacterium Rhizobiaceae members shown capable of gene transfer to plant cells
Exploring gene transfer capacity of diverse species of bacteria (TransBacterTM) in Ruta graveolens
Such Ex-Agrobcaterium mediated gene transfer has been reported so far only in few extensively worked out plant systems like Arabidopsis, tobacco, rice, soyabean, canolla, coton, corn and potato
It remains to be determined whether factors affecting transformation in Agrobacterium also affect transformation by TransBacterTM
(Broothaerts, W., Mitchell, H. J., Weir, B., Kaines, S., Smith, L. A., Yang, W., Mayer, J. E., Roa-Rodrı´guez, C. and Jefferson R. A. (2005) Gene transfer to plants by diverse species of bacteria, Nature. 433, 629-633)
GUS Plus screening
Factors that were optimized using Design of experiments (DOE)
After performing experiments according to DOE incubate shoots in X-GlcA (200ug/ml)
'Screen' blue-stained foci/ explants
GUS plus – Staphylococcus
codon usage optimized for plants, non-destructive, more sensitive
In vivo assay-GRP signal peptide directs GUSPlusTM to cell wall apoplastic space more efficiently than extensin signal peptide
Based on the observations, plant tissues could survive and continue to regenerate after incubation in a low concentration X-GlcA solution, and in the presence of the end product of GUS cleavage (indigo).
extrapolating beyond actual experimentation regions
Manually reduce runs
Design summary
pH, PEG, temperature, and interactive effect of pH and PEG (B, E,A, BE) have a significant positive influence the transformation efficiency.
Whereas ca depletion has a negative influence.
Identifying key factors influencing transformation efficiency
Production of Flavonoids by Psoralea corylifolia cultures
Time course of accumulation of daidzein (A) and genistein (B) in cultures of P. corylifolia.
J Nat Med (2010) 64:346–353
Food Chemistry 118 (2010) 128–132
Biotech and Bioprocess Engi 14: 612-618
Biotech. Bioprocess Eng. (2009)14:288-294
Rec. of Natural Products (2009) 3(1):38-45
Bioresource technology (2009)100:1833-39
Pharmacocognosy Reviews (2008)2(3):43-53
Jr of Biodiv and Eco. Sciences (JBES)(2012) 1(4): 1-9
Daidzein and genistein obtained from extract of cultures of P. Corylifolia
Bacterial strain: A4, LBA 9402
Explant used: Cotyledon, hypocotyl, shoot.
Culture conditions: Incubation at 25 oC with 16/8 hr of light and dark photoperiod
Agrobacterium rhizogenes mediated transformation of Psoralea corylifolia.
Study of genetic diversity in Psoralea corylifolia populations from Maharashtra
Location of populations of P. corylifolia from different regions of Maharashtra.
The binary matrix data was analyzed using NTSys software (Rohlf, 1994) and the statistical analysis was performed using SPSS software.
The RAPD profile yielded a total of 225 polymorphic loci with 99.56% polymorphism at species level.
RAPD analysis
Variance among populations V (A): 5.72669123200 (23.53%)
Variance within populations V (B): 18.61136306400 (76.47%)
Cluster analysis among 9 populations of Psoralea corylifolia based on Nei's genetic distances.
3 different groups are formed
1st group comprises of localities from
Pune Shrigonda and Nashik.
2nd group comprises of Kopargaon and
3rd group comprises of Nagar and Baramati.
One way ANOVA for the nine populations of P. corylifolia.
In each column, means with the same superscript letter were not significantly different, using the LSD tests (P< 0.05), and values represent mean ± SD.
Hierarchical cluster analysis based on the biochemical data in populations of P. corylifolia.
Study of biochemical diversity in Psoralea corylifolia populations
Column chromatography
Preparation of extracts:
300g of pulverized seed of Psoralea corylifolia were extracted repeatedly in 95% ethanol.
The ethanolic extract was concentrated using rota vapour. Part of the extract was coated on silica gel and was used for column chromatography
Isolation of compounds by column chromatography
Compounds identified from seed fraction of Psoralea corylifolia
The compound was isolated from stem fraction using 6:4 and 5:5 hexane: ethyl acetate gradient. The single blue fluorescent band was purified by preparative TLC and the identification of the compound was done by TLC by comparing the sample with standard. Further confirmation was carried out by LC MS and IR studies. LC MS analysis showed a single peak at 14.136 and mass spectrum peak of this compound exhibited molecular ion peak at 185. The IR analysis also showed overlapping peaks of the sample along with the standard and thus the compound was deduced as Psoralen. The molecular weight of this compound is 186.20 as per literature survey.
Compounds identified from stem fractions
Effect of methanolic and ethanolic extracts on inhibition of growth of cancer cell lines in vivo
The cell lines HEK293, S180,HT29 and A431 were purchased from NCCS (Pune) and were cultured in RPMI 1640 medium supplemented with 2% fetal bovine serum.
The cell lines were incubated overnight in a CO 2 incubator at 5% humidity and were then plated in a 96-well micro titer plate. 10μl of the extract (10-100μg) was added to each well and the cells were incubated for 24hrs.
The viability of the cells was assayed by performing MTT assay.
Anticancer assay:
SDS PAGE analysis showed dianthin 30 to be present in all the varieties
Leaves of Dianthus Caryophyllus L. contain dianthin ,dianthin 30 of molecular weight 29,500 dalton and dianthin 32 of molecular weight 31700 dalton.
Lane 1:Protein Molecular Weight Marker
Lane 2-5:Gaudina,Yellow Firato, Bizet, Flanel.
Detection of Dianthin by SDS Page
Polymerase chain reaction based detection of RIPs
Specific PCR amplifications of either pre-dianthin 30 or
dianthin 30.
Biosynthetic potential of different cultivars for Dianthin production
Different carnation cultivars - Gaudina, Yellow Firato, Bizet and Flanel were obtained from KFBioplants Pvt. Ltd.
Rooted plantlets were established in pots in the greenhouse of the Department.
Values are a mean of three replicates
17 multiple shoots per explants were obtained.
Repeated subculture did not affect regeneration potential.
A high frequency regeneration protocol was established.
Establishment of in vitro cultures.
"Biotechnology for a better future" Edts: .D'Souza et al (2004) , SAC Publications, Mangalore, India Pages 219-230
Values are a mean of three replicates
Table1: Effect of growth regulators on multiple shoots initiation.
PCR analysis conforming dianthin from in vitro cultures
Fig 6 : Dianthin amplification in in-vitro cultures
Dianthin was observed in 0.25 M and 0.3M Nacl gradient.
Both callus cultures and shoot cultures showed the presence of dianthin
M:Protein molecular weight marker, C: Callus cultures, S:Shoot cultures
Dianthin isolation was carried out by gel filtration (Sephadex g 75) using protein extract of in-vitro cultures was used (5 g of shoots, callus extracted in 50 mM phosphate buffer pH 7)
Protein extract was eluted by using a linear gradient of Nacl (0.05 to 0.3 M) for partial purification of Dianthin. Fractions were further analyzed on SDS PAGE for confirmation.
Extraction and partial purification Dianthin from in-vitro cultures.
Total RNA was isolated from Nicotiana benthemina infected cucumber green mottle mosaic virus and treated with the crude extracts of callus and shoot cultures and RT-PCR was done.
Amplification was observed only in control and not in the treated indicating the RNase activity of the extracts
Lane 1:10kb DNA marker 2: CGMMV RNA
3:RNA treated by callus 4:RNA treated by in-vitro shoots
Rnase Activity
Cucumber Green Mottle Mosaic Virus (CGMM) RNA was obtained by RT-PCR of total RNA from Nicotiana benthemina infected with Cucumber Green Mottle Mosaic Virus with CGMM coat protein AV 77 & BM 85.
Protein extract from the callus and shoots cultures were incubated with total RNA for 30min at 4C and then used for cDNA synthesis.
E: In vivo apical meristem, F: Trichomes, G:In vivo root tip, H:In vivo midvein
Histochemical localization of CPT
A: In vitro apical meristem, B:Std CPT, C: Callus, D: in vitro stem
HPLC column used was RP18. Mobile phase used for the separation of camptothecin was acetonitrile: water (40:60) .Flow rate 1.0ml/min with sample size 20 ul. UV detection at 254 nm.
Micropropagation of Chonemorpha
HPLC profiles
In virto studies and secondary metabolite production in Chonemorpha fragrance (Moon) Alston.
Hairy root initiation and elongation
Separation of CPT by HPTLC
Examination duties performed
Citation index
Ph.D. completed: 5
Ph.D. Working: 8
M. Phil completed: 2
M. Phil working : 1
1. "Effect of polyamines and Gibberellins on flowering in vivo & in vitro in garlic" Funded by DST, New Delhi. (1998)
2. "Transformation and alkaloid production in Solanum khasianum using Agrobacterium rhizogenes induced hairy root cultures. Funded by: CSIR, New Delhi. (2000)
3."Exploitation of transformed hairy root cultures of Solanum khasianum Clarke as a source for enhanced solasodine production" UGC Minor project, (2003)
4."Exploring Ruta graveolense cultures for production of furanocoumarines" Funded by UGC, New Delhi. (2006)
5. " Production of flavonoids by Psoralea cell cultures and somatic embryos" PU-BARC collaboration (2003)
6."Isolation, purification and biological activity of furanocoumarins from Ruta graveolens" Hermes Chemical Company Pvt. Ltd, Hyderabad (2007-2008)
7."Genetic transformation and metabolic engineering of Chonemorpha fragrans" UGC New Delhi, (2008-2011)
8."Exploring Dianthus caryophyllous cultures for ribosome inactivating proteins" BCUD,UOP, (2009-2011)
Research Projects completed:
1. Developed protocol for hairy root cultures in Solanum khasianum, Psoralea corylifolia and for transformed shoots in Ruta
2. Development of micropropagation protocols for Solanum surratense, Solanum khasianum, Solanum indicum, Ruta graveolense, Psoralea corylifola, Dianthus caryophyllus, Chonemorpha fragrans
3. Developed scaling up protocol for
a.solasodine production using hairy root cultures of Solanum khasianum
b.furanocoumarin production in cell and shoot cultures of Ruta graveolens
Patent granted entitled "Process of extraction of solasodine" ref no: 955/MUM/Y/310304 , dt 15.9. 2003
Patent filed entitled" process for enhancing production of furanocoumarines using tailored cell cultures (2500/MUM/2008, dt 28/11/08)
Technology development:
Break through
Ø Isolation and Characterization of Furanocoumarins from in vitro cultures of Ruta graveolens.
Hermes Chemical Company Pvt. Ltd., Secunderabad, Andhra Pradesh, India.
Dr. G.V. Ravishankar, CFTRI, Mysore for use of bioreactors. Trained the research student Asha Jacob for using different types of bioreactors
Dr. D. P. Fulzele, Nuclear Agricultural Division, BARC for Psoralea cell cultures and somatic embryos used in bioreactors for production of Isoflavonoids
Genes for metabolic engineering of phenylpropanoid pathway in Ruta
Dr. F. Bourgaud, INRA, France.
Metabolomics analysis in psoralea Dr. P J Young, Kyung Hee University, S.Korea
1. "Modular Training Course in Bioinformatics for Life Science Teachers",Organised by:Bioinformatics Centre, University of Pune,Funded by :Department of Information Technology,Ministry of Information and Communications Technology (MCIT),Government of India, September 12--October 5, 2011
2.Participated in the 2nd International Training Program organized by science and technology park, University of Pune in collaboration with Michigan State University, USA and United Nations Asian and Pacific center for transfer of technology, New Delhi in "Intellectual Property Management and Technology Transfer" Science and Technology Park, University of Pune, 19th-25th January,2003
3. Participated in the training program on "Patent Search", organized by C-DAC and TIFAC, C-DAC, University of Pune, 25th-26th July,2003
4. Participated in International training course on "Plant Cell Culture and Biotechnology" from 30th August'92 – 12th Sept.'92 held in shanghai, China, Sponsored by UNESCO. Travel assistance received from DST, New Delhi.
5. Training in the field of Genetic Transformation. Worked at Institute of Molecular Genetics, Shanghai, China from May – July'94 Recipient of UNESCO-BAC short term fellowship
Workshops attended
1.Refresher course on "Quantitative methods of analysis in Life sciences" held in the Dept. of Botany, Univ. of Pune, sponsored by UGC and ASC, Univ. of Pune, 5th June-2nd July, 1996.
2.Refresher course on "Information Technology" held at Dept. of Space sciences, Univ. of Pune, sponsored by UGC and ASC, Univ. of Pune, 4th -31st August, 1999.
3.Refresher course on "Plant Biotechnology" held at CPMB, Osmania University, Hyderabad, 1st-20th March, 2004.
Refresher courses completed:
1.Co-ordinator for Refresher course in Quantitative methods in Life Sciences" 5th June-2nd July, 1997, held in Dept. of Botany, Univ. of Pune, Pune
2.Co-ordiated National seminar on "Recent Advances in Cell, Tissue and Embryo Culture", March, 1-3, 1997, at Department of Botany, Univ. of Pune, Pune
3.Co-ordinated orientation course for course BO 201a: Cell Biology, for teachers of postgraduate centers, 12-15th Dec.1996
4.Job training for partial fulfillment of 2nd year (Biotech) B.Sc. student from Ahamednagar College & Modern college, Ganeshkhind, Pune
5.Contributed as resource person for different refresher courses in Botany and Zoology Departments
6.Contributory teacher for Dept. of Zoology and Biotechnology.
Other Activities:
Study of Rnase Activity
Antiviral Activity
Cucumber Green Mottle Mosaic Virus was used for sap inoculation on 2 month old tobacco plants.
For evaluation of antiviral activity plants inoculated with virus were treated with protein extracts of in vivo shoots, in-vitro shoots and callus.
Infection symptoms, like curling of the leaf tips, mosaic appearance on the leaf were seen less when plants were treated with in-vivo plant extracts.
Indian Drugs (2006) 42(12):1001-1003
Phcog. Res. (2010) 2(5): 298-301
Intnl J of Pharma Sci and Res (IJPSR) (2011) 2(10): 2690-2693
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Despite the well-publicized return last week of the remains of 26 U.S. fighting men and some positive diplomatic smoke signals from the Far East, relations between Vietnam and the United States are not about to move out of the deep freeze.
Vietnam certainly wishes otherwise. Late last year it formally dropped its demand that the United States honor its pledge to pay more than $3.5 billion in war reparations.


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By the time Miles Davis, Wayne Shorter, Herbie Hancock, Ron Carter and Tony Williams recorded Sorcerer in 1967, they were the most acclaimed ensemble in all of jazz. The Miles ...
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You will find Storybook Maltese located in Kansas City, MO, formerly located in the rolling foothills of the Sierra Nevada Mountains in Northern California. We have been blessed with fabulous Maltese from some of the top Champions in the USA. Having owned many dogs over the span of our lives, we are convinced that the Maltese dog is truly the most magnificent dog on Earth.
Our special little Maltese puppies are raised for Show, Service Dogs, Therapy Dogs, & Pets. All of our dogs live in our house with us as part of our family and though they each have their own roomy pen complete with a soft bed, Piddle Place, food, water, toys etc., they really don't spend much time in their pens, and can normally be found perching on our laps or at our feet if we are doing errands around the house.
Each puppy has its own special personality and we love each one very much. To tell the truth, they are spoiled rotten. Many times we've had people tell us that when they die, they'd like to come back as one of our dogs. It's very important to us to find homes for our Maltese puppies that will be just as loving as the home they were born in.
Our dogs were 'working dogs' in that we took them to work with us every day before our retirement. Not all at once, of course, but on a rotation basis. The 'Maltese of the Day' got to come to work and be an Office Maltese, greeting clients and helping to manage our stress levels. All of our Maltese are very well socialized.
We start our Maltese puppies out the same way as they are intended to become special pets for special people. We train them early to be content riding around in a purse, which comes very naturally to the Maltese Breed.
Maltese are very susceptible to separation anxiety and are intended to be with someone that can be with them most of the time and this is the kind of Maltese puppy buyer that we are willing to let one of our precious Maltese puppies go home with.
My Maltese journey began as a 10 year old girl when I first laid eyes on our neighbors Maltese. She was as sweet as she was beautiful. From that moment on, I watched every dog show on TV, suffering though all the other breeds until, at last, the Maltese graced the screen. I dreamed of having one of my own someday.
The day finally came 30 years later when my children were raised and I could afford a Maltese and I was on the hunt for my long awaited Maltese puppy. What I found is that quality, well bred, AKC Maltese puppies aren't very easy to find. I knew that I did not want to buy a puppy 'sight unseen' and have it shipped to me like cargo from across the Country and I also felt very determined NOT to support a 'Puppy Mill'.
Finally, I had my first Maltese puppy. All the time I'd spent waiting, I read book after book on Maltese dogs and how to care for them. They all warned of various difficulties and special considerations, but I was prepared. Over prepared! As the days went by I kept waiting for some issue or problem to arise. Where was the difficulty? Where were the struggles? The difficulties never came. In fact...this was the easiest dog I had ever owned in my life!
We then became obsessed with this amazing little creature known as the Maltese Dog and our journey continued from there to evolve into more than we could have ever imagined.
We now enjoy competing in AKC Conformation shows and striving to improve the breed with our dedicated breeding program.
When we produce a litter of puppies, it is for the sole purpose of continuing to develop our lines and improve the breed. Nearly all of the puppies we produce would compete quite nicely in the show ring and we must choose the best of the best to keep.
The others, we offer as outstanding pets to only the most excellent homes. When our puppies reach their new homes, they are groom trained, pee pad trained, purse trained, and crate trained to help insure a smooth transition.
We've Been Blessed At Storybook Maltese
Our breeding program is based on outstanding and diverse bloodlines. The Storybook lineage is a brilliant culmination of well-respected lines full of Champion after Champion for generations.
We have great respect and admiration for each of the amazing contributors to the Storybook line. It is a privilege and honor to carry on the many decades of hard work that are behind the Storybook Maltese line. It is with sincere gratitude that we strive onward to maintain and improve upon this work.
A great deal of thought and consideration is given to each breeding. Great care is taken when we produce Maltese puppies. Our personal goals are listed here in order of importance to us personally. We love to win in the ring, of course, but at the end of the day, that dog in the ring is going to come home and be someone's companion.
Wouldn't it be just terrible to bring your Maltese puppy home, fall in love with her, and then suddenly lose her to some health issue such as liver shunt or Granulomatous meningoencephalomyelitis (GME) etc.? Health is always our first priority.
We live with these little dogs. They're a part of our everyday lives, piling on our laps in the evening and at our heels following us around the house all day. They require a great deal of grooming, whether clipped or kept in coat. If the dog is neurotic, fidgety, won't be still to be groomed, is overly possessive and snarls, bites, or barks incessantly, eats it's own feces and leaves you wondering why you ever wanted a Maltese puppy, it's highly likely the dog will end up in a shelter. Temperament is extremely important and because many of our puppies grow up to become therapy dogs and service dogs, it's more important than ever that they have correct temperament. According to the AKC Maltese Breed Standard for all his diminutive size, the Maltese seems to be without fear. His trust and affectionate responsiveness are very appealing. He is among the gentlest mannered of all little dogs, yet he is lively and playful as well as vigorous. Correct temperament is, at least here at Storybook Maltese, ranked higher in importance than the other factors.
Every time we look at our dogs, our hearts flutter. They're just so beautiful and alluring. Our personal preference is a shorter muzzle with larger eyes that give that 'baby doll' look that captures the heart. Whenever it won't jeopardize our higher priorities, we strive toward this particular 'look'.
Correct structure is especially important in regard to the health of toy dogs to help minimize common health issues such as Patellar Luxation.
Certainly, the coat plays a very significant role in the Maltese show ring. According to AKC Maltese Breed Standard, The coat is single, that is, without undercoat. It hangs long, flat, and silky over the sides of the body almost, if not quite, to the ground. The long head-hair may be tied up in a topknot or it may be left hanging. Any suggestion of kinkiness, curliness, or woolly texture is objectionable. Color, pure white. Light tan or lemon on the ears is permissible, but not desirable.
When it comes to everyday grooming, a great coat will make life easier for both owner and dog. Coats that are curly, kinky, etc., are more likely to become a matted mess.
AKC Maltese Breed Standard dictates size to be 4-7 lbs with 4-6 lbs being preferred. Most of the families that we place our puppies with do not want a very large Maltese of 8-20 lbs, as we've seen in some places. We also do not strive to breed ultra tiny micro pocket puppies of 2-3 lbs. The tiny teacup version of Maltese is usually plagued with health issues.
Occasionally, we have a pup that will be on the smaller side. These pups require a special home to survive and thrive and extra care is taken to make sure they go to a home where they'll be properly cared for. We will never breed a dog that doesn't conform to the breed standard and therefore, all Storybook Maltese fall within the acceptable range.
Finally, part of the 'whole package' that makes up a stunningly beautiful Maltese is the black nose, eyes, and mouth and pads. It is an important part of the whole, however, it has no affect on the more important matters of health and temperament, structure and coat and that is why it's at the bottom of the list of importance. One look at our dogs and puppies will show our strength in this area as well.
Champion AKC Maltese puppies
What we do & Why we do it.
Although I fell in love with my first Maltese at age 10 and wanted one desperately my whole life, I waited until I was in a position that allowed me to give appropriate time and attention to the dog before acquiring my first Maltese.
We do not sell puppies to homes where our puppies will be left alone all day as the Maltese is the ultimate companion dog and must be with you to be happy, healthy and content. This is the very quality that makes them so perfect for work as Therapy Dogs and Medical Service Dogs.
All of our Maltese are AKC registered. All Maltese puppies are sold with a spay/neuter contract and will not leave our home until at least age 12 weeks.
If we do not currently have any available Maltese puppies, we are able to refer you to one of our many friends that may also have a top quality Maltese puppy available. It's is very important that you purchase a Maltese puppy from a reputable breeder or you may end up very disappointed with your purchase.
If you've done your research and are ready to add the love and wonder of a well bred Maltese puppy to your life, or have any questions at all in regard to the Maltese breed, please contact us at firstname.lastname@example.com and we will be delighted to help you embark on an incredible journey of your own in the world of the amazing Maltese.
Storybook Maltese


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With a motive of delivering qualitative products, we are instrumental in manufacturing and supplying a world class range of CNC Plasma Cutting Machine in Surat, Gujarat, India. Our offered machines are developed under the supervision of skilled professionals. This machine is manufactured by utilizing finest quality raw material which is sourced from the reputed and trustworthy vendor of the market. Our skilled quality experts examine this product in various quality guidelines to eliminate possible flaws. Furthermore, our customers can avail this CNC Plasma Cutting Machine at very nominal rates from us.
Last updated on : 26 April 2018


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"I don't want to box myself in. I don't want to box anybody else in. I think it's important for us to come to an agreement with the president. But this is his opportunity to lead."
— Speaker of the House
John Boehner
(R-Ohio), yesterday, on avoiding
the rapidly approaching "fiscal cliff"


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Methamphetamine and blasting materials were found Wednesday morning during a raid at a house in the Egypt community.
Methamphetamine and blasting materials were found Wednesday morning during a raid at a house in the Egypt community.
Shane Williams, 36, was one of four adults taken into custody when the Etowah County Drug Enforcement Unit conducted a raid at his home on Walker Drive off Egypt Road following a two-week investigation, Woody Johnson, deputy commander of the Etowah County DEU, said.
Agents obtained a search warrant for the house where it is believed meth was being sold, Johnson said.
During a search on the property, a quarter ounce of meth and a small amount of marijuana were found.
Three firearms and a significant amount of cash were found. A vehicle was seized.
Etowah County Sheriff's investigators assisted in the search and examined some items that possibly were stolen. However, it was not determined if any of the items were stolen, Johnson said.
"There were several items that had the serial numbers scratched off," Johnson said.
During a secondary search of the house, blasting caps and black powder were found hidden in an exhaust vent in a carport used as a shop. The explosives were wrapped in duct tape, and it was impossible to tell what they were until they were unwrapped.
"When we first saw it, we thought it might be more drugs, and then we saw the wires," Johnson said. "As soon as we realized what it was, we secured the area and called the bomb squad."
The Gadsden Police Department bomb squad responded and rendered the explosives safe.
It is not clear what the intended use of the explosives was. However, Williams is expected to be charged with possession of explosives.
He also faces charges for unlawful possession of a controlled substance for meth, a felony; and second-degree possession of marijuana and unlawful possession of drug paraphernalia, both misdemeanors.
The three others taken into custody also could face drug charges.


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A person or group of computer users that Terrorize the computer industry
with their inferior computers called Macs
with their inferior computers called Macs
A Maccie is very easy to identify, just insult Apple and you'll get a reply like this. "What did you say about Apple....I'm gonna strip a C4 to myself and take you out".
by BAM February 20, 2003
That subset of Mac users who think that Apple is responsible for all innovations. They are brain dead zealots who issue insults and worse to authors who write articles that do not give unqualified praise to Apple and its products. Maccies are approximately 70% of all Mac users.
Everything Apple does is unique and original to them no matter how many times others have done it before.
The Maccie Credo
The Maccie Credo
by Big Joe Mama May 04, 2007
A person beloging to a group of people who create film, music, printed arts and all other forms of media for Windows PC users to truncate, overcompress and pirate inferior lo-fi copies of over the internet.
by Sharon Tate July 23, 2005
buy the domain for your pet blog
A person who causes intolerable irritation for any Windroid by steadfastly refusing to just go along with the rest of the herd and use Windoze.
by Timberwof August 02, 2006


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It's that time again: The end of the year scramble to use the rest of our Flexible Spending Account (FSA) dollars. Our inventory is brimming with the latest styles, which means it's the best time to find the perfect pair of glasses. Don't wait! Stop in today. It's the perfect way to end the year.
Palo Alto, Meet Barton Perreira
Bill Barton and Patty Perreira: This dream team born out of the Oliver Peoples/Luxottica buyout several years back, are the proud founders of high fashion and luxury brand Barton Perreira. Former President of Oliver Peoples, Barton, and designer, Perreira, share a passion for independent, quality, handmade products. My personal eyewear idol, Ms. Perreira maintains a style that is fresh, youthful, sophisticated and always innovative. Handmade from the finest Japanese materials, including acetate and titanium, these styles are timeless and chic at the same time. Inspirational colors include Midnight Blue and Gypsy Rose, an ombre look of brown and a dusty red which I think is the most beautiful color on almost every woman. Between shapes, colors, sizes and materials, sun and optical, there is a frame (or two!) for everyone in Barton Perreira's collections.
Lux Eyewear is proud to introduce Palo Alto to Barton Perreira. As Palo Alto's premier destination for luxury eyewear, we strive to always offer the best in the industry, the most unique yet wearable styles in quality is that matched by few. We are proud to support independent eyewear companies such as Barton Perreira.
Stop by today, or call to schedule a private fitting with one of our talented opticians.
Salt Optics & Mykita Trunk Show
Lux Eyewear's New Online Store is Live!
We're thinning out our inventory to make room for new styles. There are some spectacular frames on sale with discounts from 50 to 75% off original prices! Designers include Balenciaga, Oliver Goldsmith, Shauns Shades, Smoke X Mirrors and Stella McCartney.
This is a great opportunity to pick up some frames at unbelievable prices. Supplies are limited so don't wait!
Tom Ford made a name for himself in the fashion world as the creative director at Gucci and Yves Saint Laurent before he launched his own line in 2006.
From suits to eyewear, Tom Ford features bold looks designed to amplify individuality.
Join us for our Tom Ford trunk show Saturday, October 22nd 11am-3pm.
Summer Clearance Sale!
If you're thinking you've never seen a frame sale at Lux, you're probably right. Our last sale was while the 49ers were still good. In order to make room for our new fall frames, we've discounted hundreds of frames up to 75% off. Choose from designers like Tom Ford, Face a Face, Lafont, Fendi, Oliver Goldsmith, LA Eyeworks, and many more. Hurry in for the best selection. Sale ends on September 30th.


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Scripture Printz
A selection of scriptures set to genuine photographs by Highland photographer George Maciver. The 6×4, 9x6 and 12x8 paper prints make excellent scripture retemory cards, while the quality of the alumini wall displays has to be seen to be believed. If you want prints without the scriptures, you can have them as well.Read More
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There is a good chance your child may have gotten a new phone or tablet this holiday season. So, how do you handle the high tech gadgets while keeping your kids safe online? Internet Safety Expert, Jesse Weinberger, joined us in studio with the rules to apply and the newest apps to be aware of.
Take a look at our video clips for the full interview.