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Study Examines Intensive Therapy and Children
Highlights from The 59th Scientific Sessions of the American Diabetes Association in San Diego
During the 1999 ADA Scientific Sessions, Tim Wysocki, PhD presented information on an ongoing study of Intensive Therapy and Children (Advances in Behavioral Medicine, Thursday June 18, 1999).
The Study is funded by the National Institutes of Health, and is designed to gain insight into some of the safety and efficacy issues of intensive insulin therapy (IT) in children. The Diabetes Control and Complications Trial (DCCT), studied only adults and teenagers. This new study will look at 160 children between the ages of 6 and 15 years, and follow them for 18 months. The study will try to identify information about knowledge, social and behavioral factors, as well as level of diabetes control, frequency of severe hypoglycemia, and weight gain. The study will try to specify what self-management behaviors might identify who would benefit from intensive insulin therapy versus standard diabetes care.
At this time 107 children are participating in the study. The children are receiving their diabetes care at one of two children's diabetes centers (at the Nemours Children's Clinic in Jacksonville, Florida or at Washington University in St. Louis, Missouri). The children are randomized into either the standard or intensive therapy group.
The standard treatment group will continue to receive up-to date diabetes care which involves 4 visits per year to the doctor and nurse, 3-4 blood sugars per day, annual free dietitian and mental health provider visits. They will have as needed phone access to the diabetes team. The hemoglobin A1c target will be less than 8%, and blood sugar goals of 70-150 mg/dl.
The intensive therapy group will have more frequent contact with the diabetes team. These participants will have weekly phone contact with the diabetes team, monthly nurse visits and quarterly physician visits. They will have as needed access to dietian and mental health provider. The intensive therapy group will have goals to keep their blood glucose levels between 70-120 mg/dl before meals, and less than 180 mg/dl after meals. Their hemoglobin A1c goal will be less than 6.5%.
The outcomes measured from this study will be hemoglobin A1c, information on severe hypoglycemia, weight gain, and use of health care services. The participants will be assessed periodically on criteria concerning psychosocial and quality of life issues. They will keep diaries of blood sugars and hypoglycemia factors, and have their blood glucose meters downloaded.
The participants who were randomized to the standard treatment group will be offered the opportunity of intensive therapy at the end of the study.
Comments: The DCCT and other studies have offered invaluable information about the importance of tight control in adolescents and adults. Those of us who care for children with diabetes, and families of children with diabetes, wrestle with the issues of where tight control fits in for children. We know that very young children are at risk from tight control due to the recurring hypoglycemia, but what about the child over the age of 6 or 7 years? We look forward to the information gained from this study to help us in our approach to the care of children with diabetes.
Posted July 3, 1999
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Last Updated: Thursday February 27, 2014 19:28:21
This Internet site provides information of a general nature and is designed for educational purposes only. If you have any concerns about your own health or the health of your child, you should always consult with a physician or other health care professional.
This site is published by T-1 Today, Inc. (d/b/a Children with Diabetes), a 501c3 not-for-profit organization, which is responsible for its contents. Our mission is to provide education and support to families living with type 1 diabetes.
By using this site, you agree to our Terms of Use, Legal Notice, and Privacy Policy.
© Children with Diabetes, Inc. 1995-2018. Comments and Feedback.


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Click here for the December issue of the Alliance for CME Almanac. There's yet another rehash of the new ACCME Standards for Commercial Support conflicts of interest documents, plus some interesting articles on CME credit for family physicians, and what the medical school and health system providers sections have been up to.
On a totally different note, I just found out that I'll be able to attend the Alliance's annual conference in January--wahoo! I've never been, but I hear so many good things about it that I can't wait. I hope to see many of you there!
For those who can't make it, I hope to blog it endlessly, as I have been with the fall Society for Academic CME meeting--I still can't believe I got so much out of a one-day meeting. The Alliance may just kill me, if it's as content-rich as I think it will be. I'm just glad this blog wasn't up and running when I went to the CME Congress last spring, which was the best CME meeting I've been to so far, but would have required volumes of blogging to do it justice!
To comment on this post, click on "comments" below. To receive a weekly update, e-mail Sue.

subset: commoncrawl


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24 Yards and 21 Inches Vintage Ivory Floral Flat Lace Trim - Scalloped Edge - 1970s
- Question? Contact the seller
Only 1 available
Item details
I was absolutely delighted to come across an estate sale in Winter Park, Florida one day early this summer. What set this sale apart from all others was there was a huge sewing room divided into three sections, one for dressmaking, one for curtain making and one for furniture upholstery. This was like walking into a dream room filled with all things sewing, and it appeared to be filled with fabrics and notions dating back to the 60's! Upon entering I counted seven sewing machines, two were of commercial grade. I quickly found three tubs of which I claimed immediately as they were filled with hundreds of yards of lace, buttons, and other sewing notions. The lace was in all sizes and colors, rows and rows neatly organized and stacked upon each other. This listing, and many more yet to come, is for some of the contents of that lace filled box.
Listed here is 24 yards, 21 inches of ivory colored flat floral lace trim. The lace is firm and measures 1-1/4" wide. The lace pattern appears to be a continual row of four square petal shaped flowers with smaller square center. The top edge of the trim is straight while the bottom is scalloped. The condition is very nice, firm and perfect for ruffling. This 'bolt' of ivory trim was packed with hundreds of yards of other lace, some patterns of which I recognized from my high school days in the 70's.
Please feel free to ask questions or to request additional photos as this item is non-returnable once sold. Ship charges will help to cover packaging materials, postage and tracking. Large yardages of lace and trim will be mailed without card holders in order to cut down on weight.
Thank you for looking!
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Shipping & policies
Estimated Shipping
EFFECTIVE 11/28/15: Any package that mails to a zip code zone that starts with a 94, 95, 98 or 99 MAY have an increase in postage that is over and above what is collected at check out. There is a considerable increase in postage for packages mailed to these zip code zones and customer will only pay for costs associated with the mailing. These zip codes zones are the furthest point from my home, or the shipping origins and usually include the states of: Northern California, Washington State and Oregon.
Currently I only ship within the US. Once payment is received I will ship within one to two business days. Tracking will be added to all shipments and insurance when necessary.
IF you live outside of the US kindly contact me PRIOR TO PURCHASE so I can review the area to be shipped to and properly assess ship charges.
IF you wish to pursue shipping out of the United States please understand that adding tracking to the package is VERY expensive. In some cases it can push the price of shipping up by as much as $40. If you elect to ship without the tracking and the package is lost somewhere along the route, I will not be able to offer a refund. I am not able to guarantee, or insure, a package if lost in route when the tracking costs have been waived in order to save money. As much as I wish I could help I am not able to risk the loss of an item and payment too, if forced into a refund.
Please know I will always work towards getting the best ship price possible as I personally don't like paying anymore than needed for this charge myself. If you wish to proceed with out of country shipping please provide me with your mailing address so that I may get an exact dollar amount to ship an item to you.
SPECIAL NOTE: CrossCreekPokeweed does not add any kind of service charge/processing charge/gas charge to shipping charges. Customers will only pay for the cost involved in shipping your package to your front door. If an overage is collected it will be refunded back to the customer.
If you are purchasing multiple items I will be very happy to consolidate and work towards reducing ship charges. Please contact me with the items you wish to purchase, prior to purchasing, so that I can work up the best possible pricing for shipping. Or, customer may elect to proceed with checkout and any overage collected for shipping will be refunded back to the customer.
Please know that I will be very happy to combine ship when multiple post cards are purchased. In most cases the first post card will ship for $3.95 and every card added to the order thereafter will ship for .50 cents. If supplies are available I will try to ship your newly purchased post cards in a clear, hard plastic sleeve to protect during shipment. Buyer can either contact me prior to purchase to adjust shipping or I can refund any overage immediately after cards are mailed. I am all for saving money on shipping!!
I accept payment in the form of charge cards and through PayPal as well.
Reservations for particular items are generally offered for a period of 24 hours. This may vary due to the popularity of the item that is being reserved. Extensions can be made while purchase details are being worked out. Please be considerate when reservations are made, if you have a change of heart and no longer wish to purchase an item, please let me know as soon as possible so the item can be returned back to open inventory.
Returns & exchanges
Items that are sold as vintage or antique are sold 'AS IS'. Any prior repairs done to these items (before my ownership) that I am unaware of, may not warrant a refund once the sale is complete. I strive to offer as much information as I can about every item and to take the best photographs possible. Please know that I am always very happy to send additional photographs or to answer any questions which may linger.


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IPL 7 auction: The big winners
MUMBAI: More than Rs 465 crore were spent during the IPL player auctions as the eight franchises gear up for the seventh season of the cash rich tourney - but bargain buys were hardly the headline makers this time around. While that can still change - money was clearly splashed on the big names in Twenty20 cricket. Here's a list of the players who were bought for a high price in this year's auction.
Yuvraj Singh (Rs 14 crore to RCB): The naysayers will say that the Punjab lad has gone for a buck too many, but Vijay Mallya seems ecstatic about the inclusion of another marquee T20 player who will add to the firepower of Virat Kohli, Chris Gayle, Ravi Rampaul and AB de Villiers. The key contributer to the World Cup and World T20 winning Team India will add more all-round ability to the team - however, the price tag may prove to be a burden if he fails.
Dinesh Karthik (Rs 12.5 crore to DD): The darkhorse in the auction and clearly being rewarded handsomely for his consistency with the long handle in the middle overs and his daredevilry behind the stumps. It was slightly startling that Mumbai Indians didn't retain him and Mitchell Johnson or used their ?Right to Match card - but Karthik is certainly laughing all the way to the bank. With 1,741 runs in 92 IPL games, he may be worth all the moolah.
Kevin Pietersen (Rs 9 crore to DD): KP is cricket's latest ?lone ranger? and he doesn?t have to care about the international calendar anymore. His mere presence in the middle will be unsettling enough for bowlers - but now they'll have to contend with a fit Pietersen for the whole season.
Mitchell Johnson (Rs 6.5 crore to KXIP): The aussie is out to prove a point, but he lets his pace and swing do the talking for him. Johnson is fast, fearsome and in insane form and is very handy with the bat as well. He will certainly add some stability to a fairly young KXIP squad.
Glenn Maxwell (Rs 6 crore to KXIP): The million dollar man from season 6 continues to be a favourite among the franchises. Though he didn?t get much opportunities in the MI camp last season, his poor showing didn't deter Kings XI from coughing up the cash. He would certainly want to prove a point and justify his worth. And being an all-rounder will help the franchise.
Jacques Kallis (Rs 5.5 crore to KKR): The old man of cricket is certainly worth every penny paid for his services. He's 38 but will be the knight in a shining armor for the KKR, hopefully once again. He has a staggering 2,276 runs in 90 IPL matches and a strike-rate of above 100. Complimenting his contribution with the bat are the 61 scalps under his belt.
David Warner (Rs 5.5 crore to SRH): Last year's surprise package - Sunrisers Hyderabad - has added loose-cannon Warner to their side. They'll be happy to see that side of him on the pitch though, rather than off it. When he's in the mood, he can devour the opposition bowlers. Also, he had a cracking IPL 6 - scoring 410 runs in 16 matches. His strike-rate was 126.93.
Robin Uthappa (KKR), Murali Vijay (DD), Michael Hussey (MI) and Mitchell Starc (RCB) - Rs 5 crore each: Uthappa's value has come down a bit but he's still handy with that bat and a reliable customer. Vijay's permanent place in the Indian team guarantees quality and Huseey aka Mr Dependable is never a bad pick. As for Starc, a good fast bowler will always cost good money.


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What is a mental health problem?
A mental health problem affects how we think, feel and behave and can have a significant impact on an individual's everyday life and physical function. Mental health problems can affect anyone of any age and currently affects one in four people in the UK at some point in their life. Mental health problems such as such as dementia and Parkinson's generally develop in older adults. There is no single cause of mental health problems and the reasons they develop are due to a complex interaction between social, biological and psychological factors.
Physiotherapy treatment can help a person with a mental health problem manage their symptoms and enhance their quality of life.
At Physio.co.uk, our physiotherapists are experienced at treating people with mental health problems including: book an appointment online today!


text: 11 May 2011Quirky singer Paloma Faith has told online magazine MyDaily.co.uk that she had laser hair removal to get bikini-ready for the summer. Talking to the magazine, Faith said: �I�ve had laser hair-removal treatment - it's amazing. It's so good. It's permanent hair removal which is great.� The 25-year-old songwriter also talked about how she stays in shape, and while she says she has got far from the perfect figure, she admitted that she is not prepared to do what it takes to get it! She talked about running just to keep from getting out of breath on stage. �I go running, but that's only because I get out of breath at gigs in-between songs if I don't. As my career has grown, the stages have got bigger, and I'm noticing how unfit I am!� she said. As the new face of the latest Tia Maria campaign, Faith is expanding her already eclectic career and she knows that image is a big part of her success. When asked whether she ever dresses down, she talked about using her image to keep a bit of privacy and admitted to therefore only dressing down in private.


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CMS rolls out researcher tool
The Centers for Medicare and Medicaid Services (CMS) unveiled a new research tool Tuesday. Being Washington, DC, the shorthand is a compilation of letters—VRDC, for Virtual Research Data Center. The tool lets researchers analyze the agency's stores of health data.
The government agency said in its announcement that the new tool will save researchers time and money, because they will be able to tap into CMS data from remote locations instead of waiting the year-long average for printed data to arrive at their doorsteps. CMS estimates that it costs more than $100,000 to ship a year's worth of Medicare A, B and D data. The new program will knock that down to around $40,000.


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Sida's Assistance to the Environment Protection Training and Research Institute, EPTRI, India
Publication date:
Sida Evaluation
Series number:
Bo Lundberg , Bo Carlsson , K P Nyati
The Environmental Protection and Research Institute (EPTRI) was created in the beginning of 1992. Its legal status is that of an "autonomous registered society", which, in contrast to a state authority, allows to provide services for payment. It is a non-profit organization and does not pay tax. The EPTRI is an independent organization but it has strong links to the Andhra Pradesh State Pollution Control Board (SPCB). Sida has assisted the EPTRI in Hyderabad, India, since its creation in 1992. This support has included the funding of consultancy services as well as equipment. Visits were made to and discussions held with members of the board, the management and staff of the EPTRI, officers of the SPCB, other state authorities, heads and researchers of governmental research institutes, managers of private service companies, industry representatives, and NGOs.


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'Star Wars' Day Marked With Blu-ray Set Announcement
Nine-disc "Star Wars: The Complete Saga" will be available on Sept. 16.
Lucasfilm and 20th Century Fox Home Entertainment marked Star Wars Day Wednesday with the announcement that a Blu-ray version of Star Wars: The Complete Saga will be available to consumers on Sept. 16.
The collection includes 40+ hours of special features, highlighted by never-before-seen content sourced from the Lucasfilm archives. Deleted, extended and alternate scenes are also part of the set as are new documentaries and various Star Wars spoofs.
Lucasfilm will preview the collection's bonus material at Comic-Con in July. The set will be released internationally on Sept. 12.
The set is divided as follows:
Disc One -- Star Wars: Episode I -- The Phamtom Menace, plus bonus features
Disc Two -- Star Wars: Episode II -- Attack of the Clones, plus bonus features
Disc Three -- Star Wars: Episode III -- Revenge of the Sith, plus bonus features
Disc Four -- Star Wars: Episode IV -- A New Hope, plus bonus features
Disc Five -- Star Wars: Episode V -- The Empire Strikes Back, plus bonus features
Disc Six -- Star Wars: Episode VI -- Return of the Jedi, plus bonus features
Disc Seven -- New Star Wars Archives: Episodes I-III
Disc Eight -- New Star Wars Archives: Episodes IV-VI
Disc Nine -- The Star Wars documentaries


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It's time to say farewell to stop motion sea monsters, spinning doll heads and poodle pimpin'--just a handful of the indelible images to come from Wieden + Kennedy and Nokia's creative partnership. The agency and client announced earlier today that they would be ending their relationship. Wieden had been the mobile giant's global AOR since 2007, but Nokia plans to "move away from an 'agency of record' model and leverage [its] wide network of agency partners," a brand spokesperson told Advertising Age.
W+K London Managing Director Neil Christie noted in a statement, "Our work with Nokia has been a rewarding and challenging creative experience. We leave this partnership with tremendous respect for the Nokia brand and wish our friends at Nokia all the best and success for the future."
Here, we've brought together some of the relationship's most memorable, and often eye-popping efforts. Just this week we saw what may very well be Wieden's swan song for the client, "Gulp." The animated piece, created with Aardman Animation, set out to break a Guinness World Record for the world's largest stop motion animation set and was shot entirely with Nokia 8 phone cameras, set up on a special rig. It was part of an ongoing series of films launched to show off the range of feats that could be achieved with the N8's technology.
Not until the film's finale do you see how massive the project really was, but the "Making of" film here dives further behind the scenes.
The large scale of "Gulp" pulled a 180 from the approach of last year's "Dot." Also created with Aardman and shot with the Nokia N8 camera, it depicted the microscopic adventures of a 9mm tall heroine.
The film below explains the big effort behind the miniature masterpiece.
Director David Wilson helped promote the N8 pink edition with this promo-like film featuring feisty Barbie clones doing very un-Barbie like things, to the Sugababes' track "Freedom."
Along with Seeper, London, Wieden conceived this interactive projection mapping event to promote Nokia's Ovi Maps. Participants' movements helped to spawn the outdoor display's dancing Nokia arrows.
The "Loop" mobile app let Nokia N8 and C7 users create bespoke music tracks out of everyday sounds. The app featured preloaded background loops or let people create their own to mix with samples recorded from real life sounds. A poodle undergoes some serious pimpin' in the app's demo below.
The shot here is from 2007's Unity effort, when Nokia and W+K put on an interactive Christmas light display, with the help of collaborators like United Visual Artists and P2 Group. Music composed by Daniel Pemberton and street performances helped bring London's Regent Street to life--but lights were the star of the show. Fourteen motion sensing LED light structures lining the road shifted color formations in response to crowd density, wind speed, weather and ambient light levels. The effort was environmentally friendly, too. The LEDs were completely recyclable and only needed a fifth of the electricity that would be used by standard light bulbs.


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Rewrite some Articles for me
Ngân sách $10-30 USD
To edit an executive business plan for formig new company
17 freelancer đang chào giá trung bình $79 cho công việc này
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(NEW YORK, N.Y., November 26, 2012) – Autism Speaks Chief Science Officer Geraldine Dawson, Ph.D. describes how the dramatic progress in autism research has paralleled increased recognition of autism's prevalence and financial impact in the December issue of the Archives of General Psychiatry published on line today. "This issue of the journal features three articles on autism," she writes in her editorial. "A decade ago, the journal published about the same number of autism articles per year."
Dr. Dawson also notes that, while the funding for autism research has dramatically increased over the last decade, it hasn't kept pace with the increasing scale of the public health challenges posed by autism. Despite an increase in research and funding, "we have not yet fully described the causes of ASD or developed effective medical treatments for it," Dr. Dawson writes. "[This issue's] articles point to an urgent need for more autism funding. We especially need more research on prenatal and early postnatal brain development in autism, with a focus on how genes and environmental risk factors combine to increase risk for ASD." Dawson also noted that research on treatment and adults with autism have been neglected and more research on these topics is needed.
Research presented in this issue reports a three-fold increase in autism risk associated with exposure to high levels of traffic-related air pollution during pregnancy and the first year of life. The study's lead author, Heather Volk, Ph.D., M.P.H., is the recipient of an Autism Speaks research grant to study autism risk and gene-environment interactions involving air pollution.
Two studies in this issue involve novel neuroimaging approaches. One confirms an association between autism and changes in immune function. This study is the first to use new brain imaging techniques to demonstrate immune function changes in adults with autism. Specifically, it documented higher than normal levels of microglial activation in the brain in adults with autism spectrum disorders. Microglial cells are the brain's first and primary immune defense. Another utilizes novel magnetic resonance imaging (MRI) data to examine the role of cortical volume in ASD, comparing young adult males on the spectrum to neurotypical control adults. The key finding is that differences in brain volume are determined by surface area, not cortical thickness, shedding light on mechanisms that might account for early brain overgrowth in individuals with ASD.
"More research is needed to develop strategies for preventing or reducing the disabling symptoms associated with this highly prevalent and costly neurodevelopmental disorder," Dr. Dawson concludes.
About Autism
Autism is a general term used to describe a group of complex developmental brain disorders – autism spectrum disorders – caused by a combination of genes and environmental influences. These disorders are characterized, in varying degrees, by communication difficulties, social and behavioral challenges, as well as repetitive behaviors. An estimated 1 in 88 children in the U.S. is on the autism spectrum – a 78 percent increase in six years that is only partly explained by improved diagnosis.
About Autism Speaks
Autism Speaks is the world's leading autism science and advocacy organization. It is dedicated to funding research into the causes, prevention, treatments and a cure for autism; increasing awareness of autism spectrum disorders; and advocating for the needs of individuals with autism and their families. Autism Speaks was founded in February 2005 by Suzanne and Bob Wright, the grandparents of a child with autism. Mr. Wright is the former vice chairman of General Electric and chief executive officer of NBC and NBC Universal. Since its inception, Autism Speaks has committed over $180 million to research and developing innovative resources for families. Each year Walk Now for Autism Speaks events are held in more than 95 cities across North America. On the global front, Autism Speaks has established partnerships and related activities in over 40 countries on 5 continents to foster international research, services and awareness. To learn more about Autism Speaks, please visit AutismSpeaks.org.


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Should you NOT receive an email containing your access data within the next few minutes, please check: are already registered in the shop? If not, please do so once as part of the ordering process. You can then select a password yourself. Once you are registered you can log in with your email address and your password in future.
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Should you continue to experience problems despite a correct email address and also not receive a "Forgotten your password" email, please contact us via e-Mail.
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Men's polo shirts are a timeless trend. The comfortable shirts not only look fashionable but are also fit for any occasion. Timberland polo shirts offer a slim fit and a perfect cut. Thanks to the classic design, Timberland polo shirts are not only great with jeans but also harmonize perfectly with elegant trousers.
Men's polos are always a good choice. They are equally well suited for a casual get-together with friends as they are fit for a relaxed evening event. Timberland polo shirts are characterized by very high quality. The typical narrow placket and collar is a real eye catcher. Made of pure cotton, Timberland polo shirts are comfortable to wear and also feel very soft. Men's polo shirts are a must for a modern wardrobe basic.
- Trendy styles and colours
- Made out of cotton
- Comfortable style
- Timeless cut


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Antiquarian and rare books, pamphlets, broadsides and manuscripts, mainly printed between 1600 and 1900, and broadly in the human and social sciences.
Catalogue 190 is in the process of being compiled. Let us know if you would like to be added to our mailing list for either emailed or printed catalogues.


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|List Price:
|You Save:
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Natural Cox-2 Enzyme Inhibitor Inflameric is an exciting new formulation designed for its use as an effective natural Cox-2 inhibitor and inflammation reducer. Recent research suggests that inhibiting an enzyme known as Cox-2 may be an effective way to reduce inflammation.
Inflameric contains oleo-resin from boswellia serrata, which contains beta-boswellic acids. These compounds have been extensively studied for their anti-inflammatory properties. In Auurvedic Medicine, a combination of boswellia serrata and curcumin (tumeric) are often used in a formulation for their synergistic effects. Curcumin is widely used for its anti-inflammatory and anti-septic properties as well.
Both curcumin and green tea are sources of powerful phenolic antioxidants that disrupt the signaling process that activates Cox-2 gene expression. Devils Claw contains Cox-2 inhibitors iridoid glycosides, such as harpagosides, clinically proven to improve joint mobility and flexibility.
MSM is the stable end-product of methyl-S-methane series of compounds that provide all life with a dominant amount of bio-available sulfur. MSM helps in the building of joint and connective tissues, and may also reduce inflammatory problems.
Gymnema sylvestre, or GUR-MAR which literally means, sugar destroyer, is added to improve sugar tolerance. This formula is designed to be compatible with low carbohydrate dieting. Balancing blood sugar may result in reducing inflammation. Vitamin C, Zinc, and an enzyme blend are added to improve circulation and may promote healthy, less inflamed joint tissue. The formulation also contains a proprietary herbal base of cats claw, devils claw, yucca, and sea cucumber, all of which may be used to reduce inflammation.
Suggested Use
As a dietary supplement, take three (3) capsules daily with a meal, or as directed by a healthcare professional. For acute conditions, the dosage may be increased to nine (9) capsules per day for the first week.
|Supplement Facts
|Serving Size: Three (3) Capsules
|Servings per container: 30
|Amount Per Serving
|% Daily Value*
|Vitamin C (from ascorbic acid)
|60 mg
|Zinc (from zinc amino acid chelate)
|5 mg
|Copper (from copper amino acid chelate)
|1 mg
|Boswellia serrata (gum resin)(Min. 65% Organic Acids)
|600 mg
|MSM (Methylsulfonylmethane, OptiMSM)
|500 mg
|Anti-inflammatory Herbal Blend
Cat\'s Claw (bark)(Uncaria tomentosa), Devil\'s Claw (root)(Harpagophytum procumbens), (Yucca schidigera), Sea Cucumber, Gymnema sylvestre Extract (leaf)
|250 mg
|Anti-inflammatory Enzyme Blend
Protease, Lipase and Amylase
|100 mg
|Curcumin Extract (root)(Curcuma longa)(Min. 95% Curcuminoids)
|75 mg
|Green Tea Extract (Min. 50% Polyphenols)
|50 mg
|*Percent Daily Values are based on a 2,000 calorie diet.
Daily Value not established.
Other Ingredients
Microcrystalline cellulose (plant fiber), rice flour, ascorbyl palmitate, and silica.
Keep out of reach of children. Store in a cool, dry place.


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On Tuesday, ABC7 took a closer look at evidence used against Yang.
The evidence is from inside Rhoni Reuter's home.
On the morning of October 4, 2007, Reuter was leaving her Deerfield condo for work when Yang ambushed her and opened fire.
Photos of that scene are among hundreds released by Lake County prosecutors showing the extensive evidence that was used to convict Yang of first degree murder.
Bullet holes can be seen in the walls of Reuter's condo.
The 42-year-old was gunned down just inside her doorway.
Firing the weapon was Yang, who was upset that Reuter was 6-months pregnant with former Chicago Bear Shaun Gayle's baby.
Yang, who occasionally had sex with Gayle, fired several shots killing Reuter and her unborn baby.
Nearly a year-and-a-half later, Yang told a froemd in a conversation that was secretly recorded that after the shooting, she spotted a photo of an ultrasound on Reuter's refrigerator and baby clothes piled on her dining room table.
Yang also said that she stole a bracelet.
Yang went on to say she discarded the weapon in a dumpster, however, police never found the gun.
Last week, a jury found Yang guilty of first degree murder and intentional homicide of an unborn child.
Several photos of the crime scene are credited with helping the prosecution in their case against Yang.
In addition to the photos, prosecutors played the recordings of Yang telling her friend how she carried out the crime.
Yang will be sentenced April 29. She faces life in prison.


text:
Le Galoubet
recommendation by allisonleah
Restaurant & Hotel owned by Celine & Franck Arribart.
Just off the main square in Arles is this quiet little restaurant and very private hotel. The service was very attentive and spoke excellent English. Our server had a vast knowledge of wines. Dinner was very good, and we ate outside on the covered terrace. Just watch the small step that runs through the middle of it.
Phone number: +33 04 90 93 18 11


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|Get It
|250 000 queries
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Know more about this business than we do? Cool! Please submit any corrections or missing details you may have.Help us make it right
Be the first AL.com user to add photos or videos of Stone Water Homes Llc
Real Estate - Development
Since 2003 the company has been providing Single-family Housing Construction.


text: Mobile Storage Georgia Mobile Storage Scottsdale Mobile Storage Scottsdale Mobile Storage Search Results We've found 1 Scottsdale mobile storage companies that deliver to Scottsdale - Click on any one of them for more information. Refine Search Size 5x5 5x10 5x15 10x10 10x15 10x20 10x30 Features Climate Controlled Drive-up Access Auto/Boat/RV Neighborhood None Found MyPorter - Your Stuff, Our Space Free Pickup! Fast Delivery! Scottsdale, GA 866-274-5242 More Info


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It's long been a grandparent's job to spoil the grandkids. However, roughly 40 percent of grandparents say they're helping their children's families with everyday expenses, according to a recent AARP survey. How should you navigate this thorny issue? Family psychotherapist Fran Walfish, Psy.D., author of The Self-Aware Parent, offers her best practices.
1. The devil's in the details. "Too many families land in my office after such transactions have occurred," says Walfish. Everyone needs to be clear on the agreement beforehand.
2. Pay for a say? No way. Nana shouldn't use money as leverage to weigh in on your parenting decisions. "Grandparents got their chance to make parenting decisions with you."
3. Make a withdrawal, deposit appreciation. For grandparents, there's an almost instinctual need for the next generation to have it better than they did. "But they are not obligated to give." Show gratitude, and the best way to do that is by spending the money wisely.


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Nikki Webcomic Page 147 – Just A Normal Girl
Chapter: Chapter 10: The new student
Location: san concord
I want to give a big thanks to Phil Hofer for upgrading my site to the latest version of Comic Easel. Phil took over support for the ComicPress WordPress theme a while back and evolved it into Comic Easel, which[…]↓ Read the rest of this entry…
As you might have guessed, I resolved my PC woes and completed my i7 upgrade 🙂 Thanks for your patience! -TK +79


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Millions of people use Microsoft Word every day and, chances are, you're one of them. Like most Word users, you've attained a certain level of proficiency--enough to get by, with a few extra tricks and tips--but don't get the opportunity to probe much further into the real power of Word. And Word is so rich in features that regardless of your level of expertise, there's always more to master. If you've ever wanted a quick answer to a nagging question or had the thought that there must be a better way, then this second edition of Word Pocket Guide is just what you need. Updated for Word 2003, this compact book covers Microsoft Word's keyboard shortcuts, user interface, commands, and tasks. Following an overview of fundamental Word concepts, the guide explains how to accomplish specific tasks, such as formatting, spelling, editing, printing, customizing, and more. It also provides reference tables so you can quickly zoom in on the information you need, like keyboard shortcuts, regular expressions, and common file locations. This handy little book makes a perfect quick reference when you want to complete tasks faster without having to plow through a thousand-page tutorial. Word Pocket Guide, Second Edition is ideal for those making the move to Word 2003. In addition, it covers Word 2002, 2000, and 97. The book includes: A brief explanation of how Word works behind the scenes An extensive guide section for common tasks as well as little-known solutions Reference tables for keyboard shortcuts, regular expressions, and common file locations If you're a Word power user, help desk staff, or anyone who wants to use advanced shortcuts or solve problems, Word Pocket Guide, Second Edition is a must-have.If Microsoft introduced a particular feature in Word 2000, 2002, or 2003, an , , or appears in the margin to the left of the ... Use the Open buttona#39;s drop-down list to open a document in different ways (read-only, open a copy, open and repair, etc.) anbsp;...
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You must register with us as either a Registered User before you can Download this Book. You'll be greeted by a simple sign-up page.
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The abortion controversy is important for what it says about our stance toward procreation and children altogether.
Cloning looks like a degrading of parenthood and a perversion of the right relation between parents and children.
It's a short step from the belief that every child should be wanted to the belief that a child exists to satisfy our wants.
Our only responsibility is to live our own life and take care of our own children.
Perhaps you could sympathize with those who seek to replace a dead child with a copy, or to copy a parent or a relative or even a celebrity.
In cloning, in contrast, reproduction is asexual - the cloned child is the product not of two but of one.
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text:
Page 14 - Hitachi Oceania Brochure 24 Pages Dec
Hitachi Construction Machinery (Australia) Pty. Ltd.
Major Operations: Product sales, service and parts support for mining, construction
and forestry equipment.
Hitachi Construction Machinery (Australia) Pty. Ltd. is a subsidiary of Hitachi Construction
Machinery Co., Ltd. (Japan) established in 1949, and a leading global manufacturer
of Hitachi branded mining and construction equipment. We are part of the global and
diversifed Hitachi Group, one of the world's largest companies and a leading researcher
and manufacturer of new technologies across a diverse range of industries.
Our position within this global network gives us unique access to vast resources
and advanced technologies unrivalled within the industry. Our commitment to a
more sustainable society has contributed to the design and manufacture of more
environmentally-conscious products and facilities. We deliver comprehensive sales,
service and parts support to Australian customers across the mining, quarry, construction,
forestry, material handling and recycling industries through a wholly-owned national
branch network.
We are also Australia's exclusive home of product sales and support for Hitachi, Bell and
John Deere equipment—three of the most renowned and innovative Original Equipment 2
Manufacturer brands in the world. In addition to the range of product brands we now
offer the Wenco Fleet Management Systems to assist mine sites in performing at their
To meet our customers' needs, we continue to expand our national support capabilities
through continued investment in both our people and our facilities. As part of an extensive
global network and with 26 locations throughout Australia, we are in a strong position to
support our customers—24 hours a day, 7 days a week.
David Harvey 3
Managing Director,
Hitachi Construction Machinery (Australia) Pty. Ltd.
For more information please contact: 1. Hitachi Excavator EX8000-6
Hitachi Construction Machinery (Australia) Pty. Ltd. 2. Hitachi Wheel Loader ZW550-5
Hitachi Rigid Dump Truck (RDT) 1300 HITACHI (1300 448 224) www.hcma.com.au 3. Hitachi Excavator ZX260LC-5


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Soft Wetting
The classical approach to wetting is to consider liquids on perfectly rigid substrates. However, in many practically important circumstances the surface is very soft and exhibits strong elastic deformations. Examples of such "Soft Wetting" phenomena are drops spreading on a gel, bending of microstructures, but also roller bearings exposed to heavy loads. This ERC will resolve how such (visco)elastic deformations influence on the statics and dynamics of wetting and lubrication. Key questions are how the contact angles and drop motion are affected by substrate rheology and the interfacial stresses of the solid.


text:
All Partners
Central Emergency Response Fund
More than 12.4 million people in the Horn of Africa are in urgent need. Help the UN help those affected by humanitarian emergencies, such as the famine in the Horn of Africa. Donate to CERF today.
Citizens for Global Solutions
Citizens for Global Solutions is a nationwide organization that inspires America to engage the world. Our members recognize that in today's interconnected world, our lives, our jobs and our families are increasingly affected by global problems. Challenges like terrorism, climate change, failed states and infectious diseases cannot be addressed by any one country alone, not even the United States. Citizens for Global Solutions believes that countries can best solve global problems by working together to find global solutions.
Club of Madrid
The Club of Madrid is an independent organization dedicated to strengthening democracy around the world by drawing on the unique experience and resources of its Members — democratic former heads of state and government. In partnership with other organizations and governments that share its democracy-promotion goals, the Club of Madrid provides peer to peer counsel, strategic support and technical advice to leaders and institutions working towards democratic transition and consolidation.
Conservation International
Conservation International (CI) applies innovations in science, economics, policy and community participation to protect the Earth's richest regions of plant and animal diversity in the biodiversity hotspots, high-biodiversity wilderness areas and key marine ecosystems. With headquarters in the Washington, D.C. metropolitan area, CI works in more than 40 countries on four continents.
Crowdrise is about giving back, raising tons of money for charity and having the most fun in the world while doing it. The Crowdrise community was named a "Top 25 Best Global Philanthropist" by Barron's. We're trying to keep Crowdrise a secret so please don't tell anyone but know that, as soon as you give, you'll be a part of something really special.
DataDyne.org was formed in 2003 by physician/epidemiologist Joel Selanikio, formerly of the U.S. Centers for Disease Control and Prevention (CDC), and technologist Rose Donna, formerly of the American Red Cross, to increase the quantity and quality of data available for worldwide public health. DataDyne.org works with mobile information technologies including handheld computers, mobile phones, the Internet, and GPS to break down the barriers to data utilization and create sustainable data flows in developing countries. DataDyne.org's premier product is EpiSurveyor, a free web-based product allowing anyone to collect data on basic cellphones for free (www.episurveyor.org). Considered to be the most widely implemented mHealth project in the world, EpiSurveyor is actively used by ministries of health in more than 15 countries, as well as many other NGOs, researchers, and UN agencies. EpiSurveyor has been adopted by the World Health Organization as a standard for electronic health data collection, and is the winner of numerous awards including the 2009 Lemelson-MIT Award for Sustainability.
Deutsche Bank Global Social Finance Group
Deutsche Bank's Global Social Finance Group provides financing solutions to enterprises that provide essential services to disadvantaged communities globally. Since 1997, the Group has structured 10 debt funds which have supported the growth of more than 100 microfinance institutions and social enterprises located in 50 countries around the world. The $330 million these funds have invested have benefited millions of low income households through greater access to financial services, affordable housing, healthcare, education and other basic services.
Dow Corning Corporation
Dow Corning provides performance-enhancing solutions to serve the diverse needs of more than 25,000 customers worldwide. A global leader in silicones, silicon-based technology and innovation, Dow Corning offers more than 7,000 products and services. Dow Corning is equally owned by the Dow Chemical Company and Corning, Incorporated. Established in 1943 specifically to explore and develop the potential of silicones, Dow Corning has grown to become a global leader in silicone-based technology and innovation.
Download to Donate
Music for Relief and Causecast have teamed up to expand the program with an innovative, and rewarding new approach to fundraising. Donations received will go directly to nonprofits working to rebuild and transform Haiti, including the UN Foundation. Download To Donate users will help keep the women and children of Haiti safe at night by providing solar-powered lights in the tent camps through UN agency partners.
Energy Foundation
The Energy Foundation is a partnership of major donors interested in solving the world's energy problems. Its mission is to advance energy efficiency and renewable energy — new technologies that are essential components of a clean energy future. The primary role of the Energy Foundation as a grant maker is to provide resources to the institutions that most effectively leverage change.


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General Motors has installed an 8.15 MW solar array on the rooftop of one of its Opel plants in Germany.
The array at Rüsselsheim – one of the largest solar installations in Europe – has an area equivalent to 32 soccer fields and will generate about 7.3 million kWh per year of energy. This represents a CO2 reduction of about 3,150 tons a year, equal to the amount of carbon isolated annually by 609 acres of pine forests, the company says.
The solar electricity produced at Rüsselsheim feeds directly into the grid of the plant and is used in vehicle production. Excess solar power is fed into the public grid of German energy company Stadtwerke Mainz.
The latest installation, together with GM's other solar arrays in Kaiserslautern, Germany, and Zaragoza, Spain, will allow the company to produce 19.1 million kWh of electricity a year – equivalent to the avoidance of 8,200 tons of CO2 emissions.
In July last year the company announced plans to double its solar output from 30 MW in 2011 to 60 MW by 2015. GM vice president of sustainability and global regulatory affairs Mike Robinson said that the goal was launched with projects like the Rüsselsheim installation in mind. GM has a second target of increasing its renewable power to 125 MW by 2020.
In May, GM announced the construction of what it hopes will be its third LEED-certified building. The engine plant in southern Brazil, equipped with numerous sustainability features including solar energy and water recycling systems, is expected to be operational by the end of 2012. Energy generated from solar panels — an amount equal to the electricity consumption of 285 Brazilian homes — will be used to power the plant's lighting on the manufacturing floors and in administrative offices, GM said.
In July 2012, the auto giant announced that its Powertrain Engineering Center in Turin, Italy, had harvested 300,000 kWh of energy from test benches – equipment that tests various measures of a running engine – within a year, and used it to power all of the facility's computers. The plant runs 15 test benches, which help engineers design more fuel-efficient engines for Chevrolet and Opel. The facility is building five more benches, GM says.


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|ognizant Communication Corporation
Gene Expression, Vol. 8, pp. 263-272, 1999
1052-2166/99 $20.00 + .00
Copyright © 1999 Cognizant Comm. Corp.
Printed in the USA. All rights reserved.
Kwan Y. Thai and James D. Forney
Department of Biochemistry, 1153 Biochemistry Building, Purdue University, West Lafayette, IN 47907
Variable surface antigens are commonly found on free-living and parasitic protozoa, yet the regulation of antigen expression and switching is not fully understood in any system. A cell line of Paramecium tetraurelia stock 51 can express at least 11 different antigens yet only one type is found on the surface at any one time. Previous studies have shown that mutually exclusive expression of Paramecium surface antigens can be overcome if two antigen genes contain the same 5' coding region. In this article we utilize a gene chimera containing portions of A51 and B51 to analyze the effect of a frameshift mutation on transcription and steady-state mRNA levels. We show that a frameshift mutation near the 3' end prevents expression of the protein on the cell surface and reduces the rate of transcription of the corresponding gene. The difference in transcription is not the result of differences in plasmid copy number. We propose that expression of the antigen on the cell surface is part of a self-regulatory pathway that influences transcription of the corresponding gene. A model incorporating the previous and current data is presented.
Key Words: Transcription; Variable surface antigen; Paramecium; GPI-anchored protein
Address correspondence to James Forney, 1153 Biochemistry Building, West Lafayette, IN 47907-1153. Tel: (765) 494-1632; Fax: (765) 494-7897; E-mail: email@example.com
Mohan B. Kumar1 and Gary H. Perdew1,2
1Graduate Program in Biochemistry and Molecular Biology, and 2Center for Molecular Toxicology and the Department of Veterinary Science, The Pennsylvania State University, University Park, PA 16802
The aryl hydrocarbon receptor (AhR), a soluble cytosolic protein, mediates many of the toxic effects of TCDD and related chemicals. The toxic effects are largely cell, tissue, and promoter context dependent. Although many details of the overall dioxin signal transduction have been elucidated, the transcriptional regulation of dioxin-induced genes like cyp1A1 is not yet completely understood. Previously, we have shown that the co-regulator RIP140 is a potential AhR coactivator. In this report, the role of coactivator, SRC-1, in AhR-mediated transcriptional regulation was examined. SRC-1 increased AhR-mediated, TCDD-dependent reporter gene activity threefold in Hepa-1 and COS-1 cells. In in vitro interaction assays, SRC-1 was found to interact with AhR but not with ARNT. SRC-1 interacted weakly with AhR in the absence of TCDD and the addition of ligand further increased SRC-1 binding to AhR. Deletional mapping studies of the AhR revealed that SRC-1 binds to the AhR transactivation domain. Finer mapping of the SRC-1-interacting subdomains in the AhR transactivation domain suggested that the Q-rich subdomain was necessary and sufficient for interaction, similar to that seen with RIP140. Using GFP-tagged constructs, SRC-1 was shown to interact with AhR in cells. Unlike RIP140, LXXLL motifs in SRC-1 were necessary for interaction with AhR in vitro and for coactivation in Hepa-1 cells. The recruitment of certain coactivators by a variety of receptors suggests possible common coactivator pools and competition among receptors for limiting coactivators. Examination of the role of SRC-1 in AhR/ARNT transactivation in ARNT-deficient mutant Hepa-1 c4 cells demonstrates that the AhR transactivation domain is sufficient for enhanced coactivation mediated by SRC-1 in the presence of a transactivation domain deleted ARNT protein.
Key Words: Ary hydrocarbon receptor (AhR); Dioxin; Coactivator; SRC-1
Address correspondence to Gary H. Perdew, Department of Veterinary Science, 115 Henning Building, University Park, PA 16802. Tel: (814) 865-0400. (814) 863-6140; E-mail: email@example.com
Y. Higashimoto,1 N. Keicho,2 W. M. Elliott,1 J. C. Hogg,1 and S. Hayashi1
1University of British Columbia Pulmonary Research Laboratory,
Vancouver, BC V6Z 1Y6 Canada
2Department of Respiratory Medicine, University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-8655 Japan
In previous studies we demonstrated that the E1A DNA and proteins of group C adenovirus are present in excess in the lungs of patients with chronic obstructive pulmonary disease (COPD). Because adenovirus E1A gene products are known to regulate the expression of many genes by interacting with cellular transcription factors, we postulated that E1A enhances the production of inflammatory mediators and exacerbates the inflammatory process in smokers' lungs. We reported that LPS-induced ICAM-1 expression in A549 cells is upregulated by E1A. In the current study we investigated whether this regulation is mediated through the ICAM-1 promoter. A549 cells and primary human bronchial epithelial (HBE) cells were transiently cotransfected with a plasmid containing the ICAM-1 enhancer-promoter linked to the chloramphenicol acetyltransferase (CAT) reporter gene (pBS-CAT-P) and either a plasmid carrying the adenovirus 5 E1A gene (pE1Aneo) or a control plasmid (pneo). To compare the effect of transient versus stable E1A expression on the activity of this promoter, we also transiently transfected stable E1A-expressing A549 cells with pBS-CAT-P. Transient cotransfection of pE1Aneo and pBS-CAT-P had no effect on basal ICAM-1 promoter activity in A549 or HBE cells. After stimulation of A549 cells with TNF-a, IFN-g, or LPS, promoter activity was increased by two- to threefold in the presence of adenovirus E1A. In HBE cells, on the other hand, E1A repressed the ICAM-1 promoter after stimulation with IFN-g and LPS with little change after TNF-a stimulation. In stable E1A transfectants, ICAM-1 promoter activity was 2 to 2.5 times higher than in control transfectants with or without stimulation with TNF-a or LPS. These findings suggest that E1A can modulate the activity of the ICAM-1 promoter in lung epithelial cells and this modulation is different in cells of alveolar origin compared to bronchial epithelial cells.
Key Words: Adenovirus E1A; Lung epithelial cells; ICAM-1 promoter; Inflammatory stimuli
Address correspondence to Shizu Hayashi, U.B.C. Pulmonary Research Laboratory, St. Paul's Hospital, 1081 Burrard Street, Vancouver, BC Canada. Tel: (604) 806-8346; Fax: (604) 806-8351; E-mail: email@example.com
Joé Attal, Marie-Claire Theron, Claudine Puissant, and Louis Marie Houdebine
Unité de Différenciation Cellulaire, Institut National de la Recherche Agronomique, 78352 Jouy en Josas, Cedex France
Specific structures found in the mRNA of picornavirus are known to allow a cap-independent translation. These structures, named internal ribosome entry sites (IRES), are also able to favor translation of the second cistron in bicistronic mRNAs. Their mechanism of action is not well understood. In the present study, two IRESs have been used: the IRES from poliovirus and a newly discovered IRES (SUR) composed of the 5' P untranslated sequence from SV40 early genes, the R structure, and a small part of the U5 region from the human leukemia virus-1 (HTLV-1). The bicistronic constructs containing the firefly luciferase gene as the first cistron and the chloramphenicol acetyltransferase (CAT) as the second cistron were driven by the Rous sarcoma virus (RSV) promoter and contained the early gene SV40 terminator. All the resulting plasmids were tested by transfection in HeLa and CHO cells. In the bicistronic mRNAs without IRES, the expression of the CAT gene was dependent on the distance between the two cistrons. The maximum efficiency in the expression of the second cistron was obtained when the intercalating RNA was composed of 30 to 90 nucleotides. This expression was deeply reduced when the intercalating fragment contained 8 or 300 nucleotides and was undetectable with 500 nucleotides. Unexpectedly, the luciferase mRNA was almost not expressed when the intercalating RNA was of 8 or 30 nucleotides. Expression of the luciferase gene occurred when the intercistronic RNA fragment was of 80 nucleotides and it became lower at 300 and 500 nucleotides. The same observations were done when the poliovirus or the SUR IRESs were added after the intercistronic spacers. However, expression of the CAT gene was amplified by both IRESs. When the CAT cistron preceded by the poliovirus or SUR IRES was introduced within luciferase cistron, 316 nucleotides before its termination codon, the IRESs were able to initiate translation of the following CAT gene irrespectively of the mRNA luciferase reading frame. Moreover, with all these constructs the highest expression level of the CAT cistron did not exceed 10% of that obtained with the same vector carrying only the CAT cistron. To identify a possible relation between the IRESs and the cap site, the CAT cistron preceded or not with an IRES was introduced 210 nucleotides downstream of the AUG codon of the luciferase gene (i.e., 258 nucleotides from the cap site) and 100 nucleotides after an added UAG termination codon. Expression of the CAT gene was not modified by the addition of the poliovirus IRES but it was strongly stimulated by the SUR IRES (the level of expression corresponded to 65% of that obtained with the same vector carrying only the CAT cistron). These results suggest that there is a cooperation between the cap and the SUR IRES and not the poliovirus IRES to stimulate translation. These data indicate that IRESs must be introduced in precise position to allow an efficient expression of the second cistron in bicistronic mRNAs.
Key Words: Internal ribosome entry site (IRES); Translation; Bicistronic mRNA
Address correspondence to Louis Marie Houdebine, Unité de Différenciation Cellulaire, Institut National de la Recherche Agronomique, 78352 Jouy en Josas, Cedex France. Tel: 33 1 34 65 25 40; Fax: 33 1 34 65 22 41; E-mail: firstname.lastname@example.com
Amanda Capes-Davis, Scott D. Andrew, Valentine J. Hyland, Stephen Twigg, Diana L. Learoyd, Trisha Dwight, Debbie J. Marsh, and Bruce G. Robinson
Kolling Institute of Medical Research and Department of Endocrinology, Royal North Shore Hospital, University of Sydney, Sydney, N.S.W., Australia
The RET proto-oncogene encodes a receptor tyrosine kinase activated by the binding of factors from the glial cell line-derived neurotrophic factor (GDNF) family to receptor-a components such as GDNF family receptor a-1 (GFRa-1). Mutations within the sequence of the RET proto-oncogene are associated with multiple endocrine neoplasia type 2 (MEN 2), an inherited tumor syndrome characterized by the development of medullary thyroid carcinoma (MTC) and other neuroendocrine tumors. Despite Northern analysis showing that RET is expressed in the majority of MTCs, the factors regulating this expression are poorly understood. To address this issue we examined RET expression in response to glucocorticoids in the TT cell line, derived from a metastatic MTC. The synthetic glucocorticoid dexamethasone was found to reduce RET expression at both mRNA and protein levels. This effect was dose responsive and maximal at 24 h. The reduction in RET mRNA was shown to be specific to glucocorticoids and was also seen in a primary MTC culture. Nuclear run-on studies revealed the reduction in steady-state RNA to be due to a decrease in RET mRNA transcription and the effect was shown to be independent of new protein synthesis or RNA stability. Dexamethasone was also found to exert an inhibitory effect upon cell growth, suggesting a potential use for glucocorticoids in the treatment of medullary carcinoma and MEN 2.
Key Words: RET proto-oncogene; Multiple endocrine neoplasia type 2; Gene expression; Alternative splicing; Dexamethasone
Address correspondence to Professor B. G. Robinson, Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, 2065 N.S.W., Australia. Tel: +61 2 9926 7267; Fax: +61 2 9926 8523; E-mail: firstname.lastname@example.com
Xiao Li Sun,1,4 Dawei Li,2 Jin Fang,1,3 Bruce Casto,3 Inge Noyes,4 and George E. Milo1,4
1Department of Medical Biochemistry and Comprehensive Cancer
Center, Comprehensive Cancer Center, 410 W. 12th Avenue, The Ohio State
University College of Medicine and Public Health, Columbus, OH
2Harvard University, College of Medicine, Boston, MA
3Division of Environmental Health Sciences, School of Public Health, The Ohio State University, Columbus, OH 43210
4Center for Molecular and Environmental Health, The Ohio State University, Columbus, OH
A linear model for human cell metastasis has been developed in vitro from chemically transformed normal human cells. The chemically transformed cells are nontumorigenic in nude mice, but can be converted to a tumorigenic phenotype by transfection with a nondirectional cDNA library or antisense cDNA to the ML-1 gene. The primary transfected cell line (TR1T) forms localized, progressively growing tumors in nude mice that do not invade into the surrounding tissue. This tumorigenic TR1T cell line could be advanced into a metastatic stage following an additional transfection (TR2M cell line) with the cDNA expression library or antisense cDNA to the ML-1 gene. Metastatic cells, selected from tumors that were attached to internal organs, exhibited an increase in invasiveness as measured in vitro using an invasion chamber. The metastatic cells also exhibited an increased expression of matrix metalloproteinase-1 (MMP-1), although MMP-1 was not part of the cDNA that was transfected into either the TR1T cells or the doubly transfected metastatic TR2M cells. These data suggest that the increase in MMP-1 expression was a secondary downstream event responding to an upstream genetic change that initiated the conversion of cells from a tumorigenic to a metastatic stage. In summary, human cell lines representing premalignant, malignant, and metastatic phenotypes have been established in culture that can be used to identify gene changes that occur as normal human cells progress to a metastatic stage during tumor development. One gene, ML-1, that is found in the expression library appears to be involved in malignant progression, because ML-1 antisense cDNA will convert chemically transformed cells to both tumorigenic and metastatic stages, and cells from both local and metastatic tumors have a reduced or complete loss of expression of the ML-1 gene.
Key Words: Metastasis; cDNA transfection; Matrix metalloproteinase
Address correspondence to Xiao Li Sun, Ph.D., M.D., Comprehensive Cancer Center, The Ohio State University, 410 W. 12th Avenue, Room 402, Columbus, OH 43210. Tel: (614) 292-3406; Fax: (614) 292-1400; E-mail: email@example.com
Ryuichi Nakashima,1,2 Huijuan Song,1 Takayuki Enomoto,2 Yuji Murata,2 Michael R. McClaid,1 Bruce C. Casto,1 and Christopher M. Weghorst1
1Division of Environmental Health Sciences, School of Public
Health, College of Medicine and Public Health and the Comprehensive Cancer
Center, The Ohio State University, Columbus, OH 43210-1240
2Department of Obstetrics and Gynecology, Osaka University Faculty of Medicine, Osaka, Japan 565-0871
Cellular responses to the transforming growth factor b (TGFb) ligand, including inhibition of cell proliferation, are mediated by a heteromeric receptor complex composed of TGFb types I and II receptors (TbR-I and TbR-II). Loss of responsiveness to TGFb, attributed to inactivation of the TbR complex, has been implicated in the development of tumors in a number of human epithelial and lymphoid tissues. To gain a better understanding of TGFb signal transduction pathways in endometrial carcinogenesis, we have investigated the role of the TbR complex by evaluating the TbR-I and TbR-II genes for mutations throughout the entire coding region in human sporadic endometrial tumors. Using reverse transcription-PCR, "Cold" single-strand conformation polymorphism analysis, and direct DNA sequencing, it was found that 1 of 39 (2.6%) and 7 of 42 samples (17%) contained code-altering changes in the kinase domain of TbR-I and TbR-II, respectively. In TbR-I, a 3-bp deletion was found resulting in replacement of Arg and Glu at codon 237 and 238 by Lys. With TbR-II, mutations were found in the kinase, the extracellular, and the C-terminal domains. No frameshift mutations were detected; however, a silent population polymorphism (AAC-->AAT at codon 389) in TbR-II was found in 19 of 42 (44%) tumor samples. These results suggest that alteration in TbR-II, but not TbR-I, has an important role in the development of endometrial carcinoma.
Key Words: TGFb receptors; Endometrial carcinoma; Mutational analysis; "Cold" SSCP
Address correspondence to Christopher M. Weghorst, Ph.D., 1148 James
CHRI, The Ohio State University, Division of Environmental Health Sciences,
College of Medicine and Public Health, 300 W. 10th Ave., Columbus, OH 43210-1240.
Tel: (614) 293-3713; Fax: (614) 293-3333; E-mail: weghorst.2(@osu.edu


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Harry Potter: Order of the Phoenix: Deluxe Action Figure: Alastor "Mad Eye" Moody
"Alastor Moody. Ex-Auror, Ministry malcontent, and your new Defense Against the Dark Arts teacher."
- More Below…
Product Description
- Cat No.
- 2941783
- 4897057880060
- Genre
- Film & TV
- Height
- 30.0cm (11.8in)
- Manufacturer
- Sideshow Collectibles
- Material
- Mixed Materials
- Packaging
- Boxed
- Series
- Harry Potter
- Types
- Action Figures
- Toys
- Universe
- Harry Potter
"Alastor Moody. Ex-Auror, Ministry malcontent, and your new Defense Against the Dark Arts teacher."
Sideshow Collectibles and Star Ace Toys are pleased to announce the Alastor "Mad Eye" Moody Sixth Scale Figure. This movie-accurate collectible figure is crafted based on the image of Brendan Gleeson as Professor Moody in Harry Potter and the Order of the Phoenix.Featuring a special moveable artificial eye and mechanical prosthetic leg.
Harry Potter fans, don't miss the chance to expand your collection with Professor "Mad Eye" Moody!
The Alastor "Mad-Eye" Moody Sixth Scale Collectible figure features:• 1:6th scale body, approximately 30 cm tall with over 30 points of articulation • Fully realized authentic likeness of Mad-Eye Moody in the movie Harry Potter and the Order of the Phoenix • Accurate facial expression and detailed skin texture • Moveable artificial eye • Each head sculpt is specially hand-painted • Three (3) interchangeable hands including: • - One (1) pair of open hands • - One (1) hand for holding wand or staff
Costume:• One (1) brown shirt • One (1) black leather vest • One (1) pair of dark blue trousers • One (1) heavily worn battle coat • One (1) black shoe
Accessories• One (1) Mad-Eye Moody's Wand • One (1) Mad-Eye Moody's Staff • One (1) Polyjuice Potion Flask • One (1) Flash for magical effect • One (1) Mechanical Prosthetic Leg • One (1) clear plastic stand with waist clip
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As a matter of fact, we are like so many others eager to know more about Chip and Joanna Gaines who are entertaining to watch but also very talented at what they do in this popular television program.
I smiled then when I discovered this video in which the talented duo play The Newlywed Game.
I am not sure that my memory would serve as well were my husband and I to sit down and play this version of the Newlywed game though I think it would be a fun game to play at a family or friends gathering.
Did you know Chip and Joanna are releasing a book that tells their story? Interestingly, when The Magnolia Story appeared on Amazon it instantly zoomed to the top of Amazon's best seller list. It is meant to share answers to the questions that fans want to know like: "Who are these people? What's the secret to their success? And is Chip actually that funny in real life?" It'd be a great gift for anyone who enjoys the series and who is interested in the couple's story. It's on my wish list.
Are you one of the myriad of people who enjoy Chip & Joanna Gaines work on Fixer Upper?
Treasures By Brenda
You never know what
I might have in store for you!
Quick Links:
Order The Magnolia Story from Amazon.


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'Godzilla' Destroys Hawaii, Headed To San Diego Next!
News footage featuring the Hawaii portion of the shoot of Godzilla has hit, and it's cool enough to spend a little bit of your time on. Also? If you're headed to SDCC this year you might want to click here
Making his first appearance in 1954 (Gojira) Godzilla is a giant monster that lives in the sea that comes from the ocean to feed on mankind. Warner Bros. will unleash his fury on May 16, 2014. Elizabeth Olsen, Bryan Cranston, Aaron Johnson, Juliette Binoche, David Strathairn, Ken Watanabe, Richard T. Jones, Sally Hawkins, Akira Takarada, Victor Rasuk, and Yuki Morita all star in the film to be directed by Monsters helmer Gareth Edwards.