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Ever a fan of questioning anyone within my reach about whatever is on their mind, (or whatever is on on mine!) I am a huge advocate for a prompt - be it in writing or verbally. In other words, asking some random question and listening to the answers spill out all over the place.
Last night before bed, we all happened to be gathered in mom and dad's room and I just couldn't resist.
So I asked...
If you were King or Queen of the land, what would your very first law be?
They got up one by one to stately stand before the crowd and bestow an answer on their loyal subjects.
Stryder: My law would be that everyone would love mom or they would go to jail for the rest of their lives! {Aw! Royal kiss up. The boy *clearly* knows who the true Household Royalty is...}
{Hopefully not inside...}
{Sounds sturdy. Have you never heard the story of the 3 little pigs, boy?}
{Both meanness and nepotism are prohibited...}
{Whoa, son. Sadistic tendencies, much?}
{What was that, buddy? Did you say there would be no stealing? Just checking...}
{Well sweetness. Suppose he wanted something else to happen on that bed after the children went off to theirs.....?}


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For lease - retail or storage
75, Gorge Road, Town Centre, Queenstown-Lakes
Price On Application
- Proposed Usage
- Industrial
- Floor Area
- 710.00 m²
- Property ID
- 1bz9bw7
An outstanding opportunity to lease a large commercial premises on busy Gorge Road. Total site area is 5119m2 with existng buildings of Showroom 602m2, Mezzanine Office 185m2, enclosed bulk storage of 710m2, and open storage 210m2. Just minutes away from Queenstown CBD, ample parking, and the possibility to split into multiple tenancies. Call today for more information!

subset: commoncrawl


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Here is my SAT14. 14 fret version of small , OOO body size guitar. This one is Mahogany back and sides and Sitka spruce top. Fitted with my standard choice of pickup - L.R. Baggs Element Active System.
$2,750 in this configuration.
Dreadnought cutaway body. This instrument can be ordered in varieties of timber combinations. Standard model include L.R.Baggs active system pickup. It comes with hard travel case. Price start at $A 2,800.
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Spiritual concepts
Higher spirit and Perceptions
The Higher Spirit together with our Perceptions cycle round and round from death to birth and death again and forms the basis for any new 'person' or a new 'soul' on birth.
On birth we are given a Personality suited to our Destiny. Personality is different from the Higher spirit. It is that permanent set of attributes - the character traits that make us who we are – a recognisable human being our conscious self.
The Higher Spirit may over many lifetimes have more than one Personality. It will have many personalities over the lives [time in-between birth and death] in which it participates.
In the diagram I have shown the Perceptions as the column and sectioned it off to show each 'life'. The Personality provides the Perceptions during a 'life' in the physical world, the Higher Spirit provides the Perceptions during the time of non physical existence – 'between lives'. The Perceptions Log is permanently attached to the Higher spirit which simply accumulates Perceptions on each life and 'death' or entirely spiritual period. On each life, the person gets a new Personality – a new set of characteristics or attributes that make the person who they are.
For iPad/iPhone users: tap letter twice to get list of items.
- A Tibetan gomchen speaks on reincarnation #016677
- Al-Ghazzali - The Alchemy of Happiness - 06 On death #018772
- Bouissou, Madame Michael - In the course of these dives a diversity of personalities frequently appears #023668
- Cohen, Leonard - Going home #023435
- Damasio, Professor Antonio - Perceptions and boundaried aggregates #014349
- Damasio, Professor Antonio - The 'mind' of a cell #014350
- Damasio, Professor Antonio - The need for defining Form boundaries #014348
- Immanuel Kant - Describes Swedenborg's communication with spirits #015125
- Motoyama, Dr Hiroshi – Healing illness #021081
- Motoyama, Dr Hiroshi – The Great Work and Spirit helpers #021079
- North Whitehead, Alfred – 08 Time and perceptions #025752
- Plato - Meno - On reincarnation and past lives #022071
- Priestley, J B - Margin Released – On Perceptions and Time #023803
- Rogo, D Scott - Leaving the Body - Contacting the Higher spirit for healing #023130
- S'RÎMAD BHÂGAVATAM – Canto 11, Chapter 03 - Liberation from Mâyâ #024755
- Seeress of Prevorst, the - On perceptions #022751
- Seeress of Prevorst, the - The log of life #022765
- Thich Nhat Hanh - The Miracle of Mindfulness - The monkey's shadow #023473
- Thomas, John F - Case Studies Bearing Upon Survival - The Indian Basket #025360
- W.Y. Evans-Wentz - The Fairy-Faith in Celtic Countries - The cocoon #014065
- Yeats, W B - Anima Mundi - On death #011794


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Dr. Geoffrey Adams
Dr. Adams grew up in a suburb of Washington DC, across the street from an Animal Hospital. He graduated from the Virginia-Maryland Regional College of Veterinary Medicine in 2000. Before relocating to New Port Richey in 2005, Dr. Adams practiced for several years in a bustling animal hospital in Silver Spring Maryland. Dr. Adams loves to educate clients. He is gentle and compassionate and really cares about your pets. He has a strong interest in dental health and spends a lot of time researching the most current treatments that will optimize the healing of your pets. When he isn't healing pets at the animal hospital, he loves playing with his two kids and spending time with his wife.
Practice Manager
Veterinary Technician


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In 1939 Their Majesties King George VI and Queen Elizabeth visited the Dominion of Canada. Thus Canada had the signal honour of being the first of the Commonwealth nations to welcome the new King and Queen in person. To honour this historic occasion and add to the warm sincerity of Canada's welcome, the Post Office Department issues three special commemorative postage stamps to coincide with the tour of Their Majesties throughout the Dominion. The stamps were printed in two colours each.
View of the National Memorial in Ottawa, designed and executed by the March family, of Kent, England. It was erected as a noble memorial to Canada's sacrifice in the Great War of 1914-1918. Surmounting an arch, through which the armed forces of the nation are pressing onward, are the figures of Peace and Freedom. To win peace and secure freedom, Canada's sons and daughters enrolled for service during the Great War. This National Memorial holds their sacrifices in remembrance of present day and succeeding generations.


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Digital Download of Things That Used to Shine
Digital Download of Things That Used to Shine
High Quality MP3 Digital Download
Written during a busy year on the road, Things That Used To Shine is an album about leaving somethings behind…and meeting others head-on. It's also the studio debut of Wild Ponies, a Nashville-based outfit fronted by Virginia natives Doug and Telisha Williams, who have previously toured and recorded as acoustic folk duo Doug & Telisha. Released by the band's newly formed independent label, DitchDog Records, Things That Used To Shine finds Telisha opening up about the skeletons that have haunted her closet for years. Grammy-winning producer Ray Kennedy (Steve Earle, LucindaWilliams) recorded the album's 12 songs in three days, running the band's harmonies through the same pre-amps once used by the Beatles. Casey Driessen, Russ Pahl, Jake Winebrenner and other heavy-hitting roots musicians also make appearances, beefing up the band's songs with everything from organ to pedal steel.


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GSW provide quality payroll services in Ireland delivered by payroll professionals.
We provide payroll services to a wide range of clients. Our experienced payroll team ensures that clients are compliant with constant changes in tax legislation.
Our experts can also advise on matters including redundancy, short-time and part-time working issues.
Our comprehensive payroll service includes:
- Preparation of weekly, fortnightly, monthly payrolls
- Calculation of PAYE/PRSI
- Calculation of pensions and share purchase plan deductions
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- Salary payslips
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- Issue and administration of P60s and P45s
- Filing of P30 & P35 returns to the Revenue Commissioners
- Facility to file returns and pay tax online via ROS


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A global coalition against corruption, Transparency International has called on countries at the bottom line of the 2014 Corruption Perceptions Index to adopt radical anti-corruption measures in favour of their people.
According to the 2014 CPI report recently published by the global accountability pressure group, corruption is a problem for all countries. A poor score is likely a sign of widespread bribery, lack of punishment for corruption and public institutions that don't respond to citizens' needs.
In this context, Transparency International called on countries at the top of the index where public sector corruption is limited to stop encouraging it elsewhere by doing more to prevent money laundering and to stop secret companies from masking corruption.
"Countries at the top of the index should make sure they don't export corrupt practices to underdeveloped countries," said José Ugaz, Chair of Transparency International in a statement.
The report has highlighted that poorly equipped schools, counterfeit medicine and elections decided by money are just some of the consequences of public sector corruption.
"Bribes and backroom deals don't just steal resources from the most vulnerable – they undermine justice and economic development, and destroy public trust in government and leaders," reads part of the report.
The report also highlighted that, in Sub-Saharan Africa, 92 percent of countries score below 50 out of 100 including Tanzania, Rwanda, Uganda, Kenya and Burundi, all members of the East African Community (EAC).
It further states that despite the government putting in place various strategies targeting on combating corruption amongst public officials, Tanzania has been highlighted as one among highly corrupt states in Sub-Saharan Africa.
Based on the report, Tanzania is among countries where public sector corruption is perceived to be greatest among those included in the list of 175 countries.
The 2014 Corruption Perceptions Index released late last year measures the perceived levels of public sector corruption in 175 countries and territories where top performer Denmark has strong rule of law, support for civil society and clear rules governing the behaviour of those in public positions.
In the 20th edition of the Corruption Perceptions Index published by Transparency International scores for Tanzania are at 31 (out of 100), Kenya (25) Uganda (26), and Burundi (20) and Rwanda (49) in the East Africa region.
Based on expert opinion from around the world, the Corruption Perceptions Index measures the perceived levels of public sector corruption worldwide, and it paints an alarming picture.
Meanwhile, the newly launched corruption report by the Research on Poverty Alleviation (REPOA) says the Prevention and Combating of Corruption Bureau (PCCB) is among leading public institutions in corruption, placed at 29 percent bribery returns ranking nationwide.
The survey was conducted in the Mainland and Zanzibar between August and September last year under Repoa's Afrobarometer Round 6 Survey, involving 2,386 respondents evaluating the ranking level of corruption in various government institutions.
But the anti-corruption crusaders hold the fourth position from the top, with the first and the runner-up positions being taken by the Police and tax collectors respectively. The third position is held by judges and magistrates, claiming 36 percent of the bribe shares.
Presenting the findings of the report titled "After more than a decade of fighting corruption: How much progress" in Dar es Salaam yesterday, Repoa researcher Rose Aiko said the public perception on government effort to fight corruption is mostly negative, though the public commends what has been done by the government in the past two years.


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>>Rams May Owe State Of Missouri Tax Money
(St. Louis, MO) — The NFL's Rams may owe the state of Missouri some tax money. The Missouri Supreme Court has ruled that an administrative hearing committee was mistaken when it previously said the Rams did not have to pay state sales tax on a special city entertainment fee added to tickets from 2007 to 2013. The Rams may owe as much as 352-thousand-dollars.
>>Lester Leads Cubs In Drubbing Of D'backs
(Chicago, IL) — Jon Lester hit his first career homer as the Cubs lit up the Diamondbacks 16-4 in the opener of a three-game set in Chicago. Lester also struck out nine to reach 2,000 for his career. However, Lester was pulled in the fifth, allowing Hector Rondon to earn the win. Anthony Rizzo went deep twice for the Cubs, who are 14-and-3 in their last 17 games.
>>Cardinals Can't Overcome Brewers' Three-Run First
(Milwaukee, WI) — Carlos Martinez gave up three runs in the first inning and the Cardinals never recovered in a 3-2 loss to the Brewers at Miller Park. Yadier Molina and Tommy Pham had RBI singles in the fourth and fifth, respectively, but that's as close as the Cardinals would get. Jimmy Nelson got the win for Milwaukee. Martinez dipped to 7-and-9 with the loss.
>>Blue Jays Double Up White Sox
(Chicago, IL) — Josh Donaldson homered and drove in three to power the Blue Jays to an 8-4 win over the White Sox in Chicago. Kevan Smith slugged a pinch-hit, two-run homer for the Sox, who came up short for the 18th time in 22 games. Mike Pelfrey allowed six runs in five-and-two-thirds innings to fall to 3-and-9.
>>Colts Have Feisty Day In Camp
(Indianapolis, IN) — The Colts are hoping for cooler heads at training camp today after a pair of skirmishes on Tuesday. It was the team's first day in pads. Wide receiver T.Y. Hilton and cornerback Vontae Davis had to be separated by teammates. Hilton later scored a TD on Davis and celebrated afterward. Davis also got into a tiff with wide receiver Phillip Dorsett.
>>Seahawks Sign Chancellor To Three-Year Extension
(Renton, WA) — The Seahawks are locking up safety Kam Chancellor. The two sides have agreed to a three-year, 36-million-dollar contract extension that includes 25-million guaranteed. The deal keeps him in Seattle through the 2020 season. Chancellor racked up 85 tackles and two interceptions in 12 games last season.
>>Report: Beyonce Interested In Houston Rockets
(Houston, TX) — One of the biggest stars in the world may be interested in buying a stake in the Houston Rockets. Bloomberg reports singer Beyonce is considering putting down some cash for the team. Representatives for the Houston native had no comment.


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Diabetes Support Group Schedule
- Posted By: Katelyn Markellos
- Category Diabetes
We are pleased to offer diabetes support groups to the Capital Region community! These support groups provide practical information and the opportunity to discuss issues related to diabetes. All are welcome to attend these free sessions and no pre-registration is necessary.Read More


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How To Upgrade A Sony Xperia P LT22i To 6.0 Version in Easy Ways of Custom ROM
We have the best way how to upgrade a Sony Xperia P LT22i to 6.0 version in this article. As we know, the Android 6.0 update refers to the latest update of Android operating system. It is called as Android 6.0 Marshmallow. This new operating system is very interesting to be tested because Google as the developer of Android always surprises its users every year. Luckily, we want to share about what our team achieved this year. They have found easy way to upgrade your Sony Xperia P in the easiest ways. Without further ado, let us check out them all below.
Custom ROM
how to upgrade a Sony Xperia P LT22i to 6.0 version is by using custom ROM. For your information, ROM stands for Read-Only Memory, which is manufactured by a company or third-party. The benefit of custom ROM is that you are able to get the latest update like Android Operating System without waiting from the manufacturer of your phone. To get the Marshmallow update, you have to do a thing called flashing new ROM. It means that you will install a new OS for your Android. Let us go to the steps or instructions for flashing new ROM for your Sony Xperia P LT22i below.
Marshmallow Features
Before getting the easiest way of how to upgrade a Sony Xperia P LT22i to 6.0 version, you have to know some new features of Android Marshmallow firstly. This year, Google gives all Android users with a new Mobile payment method, which is called as Android Pay. Secondly, this new operating system also offers better RAM management that became frustrating all android users recently. In addition, the new OS also becomes faster and smoother than the Lollipop version. Thanks to Google because they have given us fingerprint support just like other modern brands. This manufacturer also remembers increasing the battery performance up to ninety minutes.
Now, this is the first step how to upgrade a Sony Xperia P LT22i to 6.0 version. You must check the current version of your Android Os firstly. Make sure you have the latest version of Android OS, which is version 5.0 Lollipop. After that, connect to a Wi-Fi connection that is available on your coverage. Wi-Fi connection is more stable than using the data connection of your phone. Simply click on the Software Update on the general info of your Android. At last, you will see the update available and confirm it by using yes button.


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Lady Sun Devils beat Ducks for 8th consecutive victoryPosted: Updated:
TEMPE, Ariz. -- Briann January scored 12 of her 14 points in the first half as Arizona State won its eighth consecutive game by beating Oregon 68-56 last night.
Danielle Orsillo added 13 points, Dymond Simon 12 and Becca Tobin had 11 points and six rebounds for the Sun Devils, now 16-6 overall and 8-2 in Pac-10 play.
ASU's streak is its longest since winning nine straight in 2007. The Sun Devils won a school-best 10 straight games during the 2005-06 season.
ASU's pressure defense forced the Ducks into 26 turnovers, leading to 31 points.
The Sun Devils ran off nine straight points to take a 52-33 lead with 12:42 remaining and Oregon did not get closer than 12 until the final minute.
(Copyright 2009 by The Associated Press. All Rights Reserved.)


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|» Products
|» Physics Instruments
|» Suction Pump
Suction Pump -HLSI
Suction is the flow of a fluid into a partial vacuum or region of low pressure
fixed to polished seasoned teak wood base, single barrel made from steel pipe with stock cock. Pump plate made of aluminium metal.
Diameter of Pump Plate A B C
125 150 250


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Back to School!
It's almost time for back to school-don't forget to call and schedule a cleaning appointment for your kids that will work with the school schedule!
Also, you can visit the ADA's website for kids, 2min2x.org, for lots of tips on keeping kid's teeth clean.
Leave a reply


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SuperSmiler Profiles
Monday, September 14, 2015
Photo Day FUN at Oak Grove School!
Kate Nagle and her team had a wonderful time back at Oak Grove School! We are delighted to be providing your school portraits this year!
Please keep Kate in mind when thinking about your family portraits or special events. You can see more of her work at KateNaglePhoto.com.
Posted by Linda Russell at 9:23 AM
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Bangor is the actual or alternate city name associated with 2 ZIP Codes by the US Postal Service.
Select a particular Bangor ZIP Code to view a more detailed map and the number of Business, Residential, and PO Box addresses for that ZIP Code. The Residential addresses are segmented by both Single and Multi-family addessses.
Each individual Bangor ZIP Code profile also includes Demographic Data including Population, Housing, Income, Households, and Growth Rates.Photos of the local Bangor, ME area
|Bangor, ME - Standard ZIP Codes
|Bangor, ME - PO Box ZIP Codes (NO DEMOGRAPHIC DATA)
The Bangor ZIP Code maps and basic ZIP Code information such as the number of Business, Residential and PO Box addresses in the ZIP Code are updated 4 times per year. Last update: August 1, 2018.
The DEMOGRAPHIC DATA for each Bangor ZIP Code is updated annually. The last update was on July 1, 2018 and includes:


text: This program exposes the volunteers to various eGovernance initiatives both at the central as well as state level. Volunteers will get an understanding of the various software applications developed by the Himachal Pradesh Government to better serve the citizens. We will also arrange lectures and interactive sessions with bureaucrats, political leaders and academicians.


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NATO and Russian negotiators agreed today on how to deploy Russian peacekeepers in Kosovo, defusing a diplomatic standoff that over the weekend prompted NATO to block the flight of Russian troops to the battered Serbian province.
The accord, coming after 11 hours of talks here, opened the way for swift deployment of a Russian Kosovo contingent of 3,600 troops within a NATO-led force that eventually will total more than 50,000. It also appears to mark resumption of cooperation between Moscow and NATO in the region following last month's unexpected occupation by 200 Russian troops of the airport in Pristina, the Kosovo capital, which was to have served as NATO peacekeeping headquarters.
NATO considers Russian participation in the peacekeeping mission an important means of reassuring Kosovo's shrinking Serbian minority that it will be secure in the midst of hundreds of thousands of returning ethnic Albanian refugees who were driven from their homes by Serb-led Yugoslav forces during the 2 1/2-month Kosovo war.
The latest dispute over the Russian peacekeepers demonstrated a new level of mistrust of Russia by NATO leaders and a determination by the alliance to nail down details of Russia's role in Kosovo before allowing it to send more troops to the Pristina airport.
Late last week, NATO called on Hungary, Bulgaria and Romania to deny Russian military planes an air corridor to Kosovo on grounds that Moscow was trying to change the terms of a peacekeeping agreement already reached at Helsinki by seeking to gain more autonomy for Russian commanders in the province. Russian military officials responded that NATO's blockage of overflight rights was a provocation, and they threatened to send reinforcements to Kosovo by train if necessary.
NATO officials said Russia won no concessions today on where its troops will be stationed and to whom they will report. "This is pretty much what we thought it would be from Helsinki," said one NATO officer.
The Russian troops will be scattered in noncontiguous sectors under U.S., French and German command, as previously agreed, NATO officials said. Russian military leaders had hoped to deploy their units next to each other, but NATO objected, saying that this would give Russia a de facto sector of its own and potentially too much independence.
On the other hand, each link in the chain of command will include a Russian liaison officer--16 in all--enabling Moscow to say its troops do not report directly to NATO. In addition, Russian soldiers may refuse to carry out NATO-ordered tasks if they conflict with orders from Moscow; for instance, the Russians are known to object to using military force to apprehend war crimes suspects in Kosovo. "They will have to follow their own national laws and national policies," Gen. Wesley K. Clark, NATO's supreme commander, told the Associated Press at his headquarters in Mons, Belgium.
Russian Foreign Minister Igor Ivanov described the lingering issues that prompted today's talks as technical and relatively minor, but NATO's insistence that everything be worked out beforehand illustrated the degree to which Russia's actions have strained its credibility in Western capitals over the past month.
First, Russia dispatched troops to take over the airport in Pristina without NATO's consent or knowledge. Then it sent two strategic bombers on an exercise within theoretical striking distance of the United States. Finally, after agreeing in Helsinki to disperse their troops in three sectors of Kosovo, Russian negotiators insisted on putting some into the Italian-controlled sector, a move that would link Russian troops in the French, German and Italian zones.
"The Russians aren't playing straight with us," one NATO official said today. "They are playing games." The Russian newspaper Vremya suggested that NATO now will double-check Russia's every move. "Jitters are now the order of the day," the newspaper said, and it predicted a new "chilly" war between the old Cold War rivals "on the level of small, dirty tricks."
NATO wanted "to show us who is boss," said Vladimir Lukin, chairman of the foreign affairs committee of the lower house of the Russian parliament. "They are showing us that the affair starts but does not end at the [Pristina] airport, that there are many airports in various countries and these countries behave obediently."
Besides patrolling part of the U.S., German and French sectors, Russia will have 750 troops stationed near Pristina airport. A Russian will command the airfield, but NATO will control air operations, NATO officials said.
Staff writer Dan Morgan in Washington contributed to this report.


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Have you ever wondered what it would be like to just click with someone?
Well the authors of Click: the Magic of Instant Connections, Ori and Rom Brafman have made it simple.
No more trying to put your finger on it, it boils down to traditional psychology.
The magic ingredient in that 'CLICK' is vulnerability. That's what they believe and you know what I say the same.
I know right, no-one wants to look vulnerable and boy I know it's the last thing you are willing to do. But before you stop reading, consider this...... your human.
So when you meet someone for the first time, let's imagine it's the person that is interviewing you for that dream job, the person your pitching a fantastic idea to, the bank manager or you're on a date. It's easy to forget that the person sitting opposite you isn't just someone you're trying to impress. But they're human too.
Giving yourself permission to be vulnerable helps the other person to trust you, precisely because you're putting yourself at emotional, psychological or physical risk. Other people tend to react by being more open and vulnerable themselves,' Brafman and Brafman say.
'The fact that both of you are letting down your guard helps to lay the groundwork for a faster, closer personal connection. When you both make yourselves vulnerable from the outset and are candid in revealing who you are and how you think and feel, you create an environment that fosters the kind of openness that can lead to an instant connection – a click
And who does not want to click? My clients tell me they are looking for that all elusive Click!
Tell me your not...
It starts getting a little more interesting when other elements are at play when it comes to the 'click' Brafman and Brafman also believe that couples with similar interests, levels of education and social standing tend to be more drawn to one another. But it's seeing someone as 'real' and down-to-earth that can often make this their most attractive quality; something that you can't communicate until you've chosen to open up.
My client Sameer took what he thought was a risk, showed his vulnerable side, he discovered something about Henna that helped him confirm that she was the one. You can guess what happened next.
So you see that is what is so interesting about vulnerability and its effect on the 'click'. Compared to everything else going on, like wondering whether or not you're actually, pretty enough, young enough, tall enough, being funny enough, your vulnerability factor is something that you can actually influence the outcome.
There are five levels of vulnerable language, and when we eventually access the final stage with a romantic partner, studies show that couples feel safe and extremely connected.
The five stages of vulnerability
So, the next time you're meeting someone new– try opening up a little. BE human, take off that mask, present you, be vulnerable, because that 'real' relationship really could be right under your nose.
If you need a helping hand navigating the maze of life, I am here to help, your just one click away.
Until Next Time... BE YOU, BE REAL, BE BRILLIANT!
With Love
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SXSW To Premiere AMC's "Preacher," Releases New Still
Ahead of its premiere on AMC sometime in mid-2016, "Preacher" will get the world premiere treatment at the South by Southwest festival. Variety announced that the adaptation of the cult classic Vertigo series will be part of SXSW's film programming, which runs from March 11-19. Date, time and venue information has not yet been released for the "Preacher" premiere, although the show's page on the SXSW website indicates that info will be released this month.
The show's page also includes a new still (above to the right) from the AMC series. The pic shows series lead Dominic Cooper, who plays preacher Jesse Custer, lighting up in a church. The announcement included a synopsis for the episode, including cast and crew information.
"Preacher" (World Premiere)
Directors: Seth Rogen, Evan Goldberg, Screenwriter: Sam Catlin
Preacher is a supernatural, twisted and darkly comedic drama that follows a West Texas preacher named Jesse Custer, who – along with his ex-girlfriend Tulip and an Irish vagabond named Cassidy – is thrust into a crazy world, much bigger than he is.
Cast: Dominic Cooper, Ruth Negga, Joseph Gilgun, Ian Colletti, W. Earl Brown, Lucy Griffiths
The festival will also host the premieres of Paul Reubens' "Pee-wee's Big Holiday," "Tony Robbins: I Am Not Your Guru" and "Beware the Slenderman." The SXSW film festival takes place in Austin, Texas from March 11-19.
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Billionaire Nina Wang 'not clear' on intention in will, appeal court hears
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A court should not rule that late billionaire Nina Wang Kung Yu-sum's fortune should be managed by a charitable trust given that she specified no such thing in her will, the Court of Appeal heard yesterday.
Barrister Frank Hinks QC, for the Chinachem Charitable Foundation, made the remark as the foundation challenged a Court of First Instance ruling that it would hold Wang's HK$82 billion estate as a trustee, rather than as an absolute beneficiary.
Hinks said that when Wang drafted her will in 2002, she stated that she wanted the money to be used for both charitable and non-charitable purposes. But she was not clear on how she wanted the money distributed.
Hinks said the will was "homemade" and not drafted by a lawyer.
"She could have hired one but she elected not to do so," he said. Because of this, he added, "the court should not do what she chose not to do".
Hinks said Wang only stipulated that she wanted to use her fortune to set up a prize similar to the Nobel Prize, and to continue the foundation's charitable work.
Developer Chinachem Group, which made up most of Wang's estate, was worth more than HK$93 billion in 2012, Hinks said. After deducting HK$11 billion in liabilities, the value of the estate was about HK$82 billion.
The group made a profit of about HK$2.5 billion that year, the court heard, but only HK$100 million went to charitable work.
A businesswoman as successful as Wang would not want her money used only for the two purposes she stated in the will, Hinks argued. He said that where uncertainty existed over the will, the court should not order something that was not in the will.
But Simon Taube QC, for the justice secretary, who acts as protector of charities, argued that it was Wang's desire to have her estate used for charitable work and said the lower court was right to rule that the foundation held her wealth in trust.
Court of Appeal vice-president Mr Justice Johnson Lam Man-hon, Mr Justice Peter Cheung Chak-yau and Madam Justice Susan Kwan Shuk-hing reserved judgment to an unspecified date.
The court battle is the latest of many over the legacy of the colourful Wang, who died in 2007, and husband Teddy Wang Teh-huei. It started with a probate case over Teddy Wang's fortune after he was declared dead in 1999, nine years after he was kidnapped and never seen again. Fung shui master Peter Chan Chun-chuen was jailed last year for using a forged will to try to claim Nina Wang's billions.
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The Golden State has so many amazing places to enjoy. I've recently returned from a photo trip to the Eastern Sierra. These are my favorite shots from a few of the highlights of that trip.
Some random images of beautiful things that drew my eye.
This gallery is a rotating selection of my current favorites. Enjoy a sample of some scenes from around the world.


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Boards to the left of Michal Rozsival. Christopher Higgins dutifully to the right. And only Michael Rupp, 38-goal scorer in 364 NHL games, in front of the Rangers defenseman.
"Arty [Artem Anisimov] falls down and it's three-on-two initially, but Higgy [Christopher Higgins] is back [in the play] by above the blue line," said John Tortorella. "And Rosey even takes a look.
"It's really an even-strength rush that we need to close out. For some reason he sunk into the middle."
Rozsival played it like the Penguins had numbers, played it like a guy whose days as a Ranger should be numbered. Rupp closed to past the face-off dot and ripped the third-period tiebreaker past Henrik Lundqvist.
The Rangers played a better game than most during this 6-12-1 slide that has followed their 7-1 start, still couldn't get the job done in a 5-2 loss, their second in three nights to the Stanley Cup champions.
"We need another big offensive play, a big defensive play, a big save, that's the fine line of winning in this league," said Tortorella. "Right now we're not getting it done."
For 19 games, we would add, practically a quarter season of a team that, on the ice, is proving pretty much what it is on paper. While it always is arguable whether players are playing to their limits, there is no question the Rangers have spent to theirs, wedging themselves up against a $57 million cap that has become their rock and hard place.
For $56.85 million, the Rangers have a team with just one above-average scorer, Marion Gaborik; no true No. 1 center; and no real No. 1 defenseman. Worse than an indictment of how poorly Glen Sather has cut the pie, it actually is a depressing portent of years of mediocrity.
The Rangers will pay Ales Kotalik, who can barely get on the ice, $6 million for two years after this one. They owe Chris Drury, a second-line player whose best years were in Buffalo, $8 million next season and $5 million for the one after that. And Rozsival and Wade Redden's untradable deals will choke the Rangers' chances to improve themselves until they are swallowed whole.
The contract of Redden, paying $8 million this year, $6.50 million the following two years, and $5 million for the two after that, has to be jammed past Jim Dolan's gag reflex and down his hatch. The contract of Rozsival, worth $6 million this year, $4 million for 2010-11 and $3 million for 2011-12 has to be salted and pitch-forked past the owner's teeth.
Undoubtedly bitter for Dolan has been the taste of the deals of Larry Brown, Jerome James, Jared Jeffries and Eddy Curry. Even worse has been having to pay the guy who decided to lavish them, Isiah Thomas, after his firing. But the faster Dolan and Sather reluctantly concur that their team can't get better as long as Rozsival and Redden are on it, the quicker the Rangers can recover from Sather's grievous mistakes of the summer of 2008.
We don't know what he was thinking when, having already watched three years of Rozsival, the GM wanted four more. We don't know who told him that Redden, who had played been dead on his feet for much of the past two years in Ottawa, still had something left. But we also don't know any other way out from two of the dumbest Garden signings ever but to waive both players and let them finish out their contracts in Hartford.
The $14 million the Rangers are paying Rozsival and Redden this season could, in today's NHL market get them Johan Franzen ($5.5 million from Detroit) Dustin Penner ($4.25 million from Edmonton) and still have $4 million left for Chicago's Patrick Sharp.
Gaborik was excellent again last night. And again no other Ranger scored. But then what do you expect for $56.875 million?


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Dear Greg1203,
Welcome to the IMSLP! We would like to thank you for contributing.
Please visit our Contributor Portal to learn more about contributing to IMSLP.
The Tutorial provides you a basic introduction to using this site. If you wish to contribute music scores, you will find all the details in the page How to Submit a Score. In addition to that, it is advisable for you to have a basic understanding of copyright law. In case you aren't able to find something you want to know in those pages, you can leave a note on the forums.
Please consider putting the Babel tags on your user page, to tell others which languages you speak. You can also use the Babel system to find users who speak a certain language.
You can communicate with other users by placing a message on their talk page (click on a user's name, and then on Discussion), just like I did on your page. When logging on the next time, this user will get a notification of a new message. Replying to these messages can be done on your own talk page, or on the corresponding user's talk page. Note that there will be no notification for the other user when replying on your own page. It's always a good idea to sign your comments with four tildes (~~~~), which automatically adds your user name and a datestamp.
If you want to discuss something with the whole community, you can post a message in the IMSLP forums. For very important messages, please contact the staff directly via the IMSLP staff contact list.
Frequently asked questions can be found on the FAQ page.
We're looking forward to your next submission!--Emeraldimp 18:38, 18 June 2007 (EDT)
Also, if you want the pieces you uploaded to appear on the site (and not be deleted!) you'll have to create a composer page for the composer, and then work pages for each of the pieces. Review the score submission guide for more info. Thanks! --Emeraldimp 18:38, 18 June 2007 (EDT)
(P.S. This is the case for your 2007 uploads as well - they have never been accessible)


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A Severe Thunderstorm Watch is in effect for Dutchess and surrounding areas Saturday until 9 p.m.
Conditions will be conducive for the development of severe storms which can contain heavy rain, strong winds, small hail and dangerous lightning.
If storms affect your area, remain indoors, if possible.
The storm chance starts at around 5 p.m.
There will be patchy fog after 1 a.m. Otherwise, it will be mostly cloudy overnight with a low around 62.
Sunday and Monday will both be mostly sunny with a high in the low 80s.
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The American Dream. It means different things to different people. I love to start each school year off by asking my students to explore the concept of "The American Dream" and define it for themselves. I usually start by asking them what the first thing that … Continue reading


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Team:Sydney Australia/Experiments
- 1 Agarose Gel Electrophoresis
- 3 Restriction Digest
- 4 Ligation
- 4.1 Golden Gate Assembly
- 4.2 Heat-shock Transformation
- 4.3 P.putida Competent Cells
- 4.4 E.coli Competent Cells
- 4.5 Transformation of P.putida bacteria via Electroporation
- 4.6 IPTG Induction
- 4.7 NBP Assay
- 4.8 LB Media
- 4.9 VIOGENE Gel/PCR DNA/RNA extraction kit
- 4.10 Bioline ISOLATE II Plasmid Mini kit
- 4.11 Plasmid MidiPrep - 100 mL
Agarose Gel Electrophoresis
Gel electrophoresis is used to separate DNA based on their size and mobility through agarose. It relies on the fact that DNA is negatively charged, and that a uniform electric field can be applied across the semi-permeable agarose gel.
For a 1% agarose gel:
- Add 1 g of agarose to 100 mL TBE.
- Gently mix and microwave heat until dissolved.
- Prepare gel tray. Depending on the tank, use masking tape or end-formers to seal the two ends of the gel tray. If using end formers, seal the ends by pipetting a small volume of gel solution into the contact between the end formers and tank and allow to set.
- Allow the gel solution to cool and add 0.5 µL of Gel Red and pour the gel into the gel tray. Alternatively, exclude the Gel Red and stain with Ethidium Bromide after running the gel.
- Insert the well comb into the appropriate position in the gel.
- Once set remove the comb, tape/end formers and place into electrophoresis chamber and submerge in TBE.
- Mix samples with loading buffer (use 1:5 ratio of loading buffer to sample) and pipette into wells.
- Apply 120-180V, depending on time constraints and desired resolution.
The Polymerase Chain Reaction is used to amplify segments of DNA to high concentrations as a screen for the presence of the target or for further manipulation. The following protocol is readily tweaked depending on the specifics of the template DNA, primers and polymerase of choice.
PCR of multiple samples is sped up significantly by making a master mix; the volumes below are for one 50 µL reaction, but the volumes would be multiplied by the number of reactions required and mixed together in a single tube.
- 5 µL 10x Pfu buffer/NEB Thermopol Buffer
- 1 µL dNTPs at 10 uM (final 200mM)
- 1 µL primer (F) (final 0.5-1uM)
- 1 µL primer (R) (final 0.5-1uM)
- 40.5 µL sterile MQ water
- 0.5 µL Pfu/Taq polymerase
- Aliquot master mix into PCR tubes (49 µL) then add 1 µL of the template DNA. Alternatively, if performing a colony PCR, aliquot 50 µL of master mix into a tube and resuspend cells directly from the plate into the tube. (Only dip the toothpick 3-5 times in the master mix).
- Start the thermocycler at the setting desired. Cycles must have a peak high enough to denature template DNA, a trough low enough to allow annealing of primer pair, followed by an intermediate stage for the optimal polymerase activity.
NB. Keep enzyme on ice and return to the freezer ASAP.
Restriction Digest
Restriction digestion employs restriction enzymes which recognise specific sites in a DNA sequence and will cut the strands at those sites. This may be used for diagnostic purposes by separating DNA into fragments of predictable lengths or for assembly by generating short overlaps at the restriction sites.
- Add 5 µL of appropriate 10x buffer to a microcentrifuge tube. The buffer choice depends on the restriction enzyme used and can be checked from readily available tables.
- Add DNA sample solution (~1 µg DNA).
- Make tube up to 49 µL with reverse osmosis water.
- Add restriction enzyme; 10 U activity is sufficient, which is normally the activity of 1 µL restriction enzyme solution. The restriction enzyme volume should not be more than 1/10 of the total reaction volume.
- Incubate for an hour at the optimal temperature for restriction enzyme activity; again, this should be checked from relevant data tables.
Restriction digests with two enzymes simultaneously are also possible and were performed over the course of the project. This involves the same protocol as above except that the reaction mixture is made up to 48 µL in step 3. This is only possible if the enzymes have compatible reaction buffers and optimal temperatures; if they do not, then you must perform two sequential digests with a purification step in between
Ligase facilitates binding between complementary sequences of DNA. Ligation allows fragments of DNA with sticky ends to be joined together, but the enzymes also has other uses like in Gibson Assembly. Ligase is not thermostable, so there is an efficiency trade-off between increasing the rate of ligation and the rate of ligase degradation at higher temperatures. The following protocol is for the ligation we performed most often; that of cloning an PCR product into a plasmid prep. The following volumes thus hold only with the concentration of our plasmid prep. The ligation reaction is quite flexible, and the following protocol can be applied to general ligations, as long as care is taken to maintain a ~3:1 molar ratio of insert:vector, and that the total DNA concentration does not exceed 10 ng/µl.
- Digest plasmid e.g. Use 250 ng in digest volume of 100 µL although there is a wide acceptable range for this.
- At the same time as step 1, digest insert(s) e.g. Use 1 µg in 100 µL digest.
- Purify both digestion samples using DNA extraction and purification kits (e.g. VIOGENE)
- Combine on ice 2 µL of NEB 10X T4 ligase buffer, 8.5 µL purified insert, 8.5 µL of purified vector, and 1 µL of T4 concentrated DNA ligase. Make sure ligase enzyme remains in ice at all times.
- Incubate the sample for an hour at room temperature, or overnight at 4°C.
To avoid column purification of digestion samples, heat inactivated digestion samples can be used for the ligation. In this case, make sure that:
- Digestion is set up in small volume e.g. 25 uL
- Check that the restriction enzymes used can be heat inactivated.
Golden Gate Assembly
Golden Gate Assembly (GGA) is used for cloning one or more inserts into a vector by designing overlapping ends which can join desired DNA together after cleavage by non-palindromic restriction enzyme, BsaI, followed by ligation to seal the DNA together.
To set up a 15 uL GGA reaction:
- Add 1.5 uL 10X T4 DNA ligase buffer (50mM Tris-HCl, 10mM MgCl2, 1mM ATP, 10mM DTT, pH 7.5@25°C).
- Add 1 uL BsaI/BsaI-HF (200 U)
- Add 1 uL concentrated T4 DNA ligase (2000 U)
- Add equimolar amounts of insert(s) and digested vector e.g. 50 ng cut vector
- Make up the solution to 15 uL with MiliQ water
- Set up thermocycler to cycle between 16 C (1 minute) and 37 C (1 minute) for 30-35 times
- After the termination of heat cycle, incubate sample at each 50 C and 80 C for 5 minutes
- Transform 3-8 uL of GGA sample to a suitable host
Note: Assign volumes of insert and vector such that to avoid total volume of more 15 uL.
Refer to NEB Golden Gate Assembly or Sydney iGEM Golden Gate explainer for further information on GGA method and design.
Heat-shock Transformation
Transformation is a technique by which DNA may be inserted into competent cells. Transformation occurs naturally when cells uptake and express exogenous DNA from their environment. Heat shock transformation uses a rapid change in temperature to cause plasmids to enter cells via pores in the membrane. The introduced DNA will often contain a marker gene (usually antibiotic resistance) so that successfully transformed cells can be grown on selective media (normally an antibiotic), which corresponds to the marker gene.
- Set-up a heat-block or water-bath at 42°C.
- Retrieve aliquots of cells from -80°C freezer. Thaw on ice.
- Add 1 pg-100 ng (1 µL - 5 µL) of plasmid DNA into cell suspension.
- Incubate tubes at 42°C for 30-45 seconds.
- Return to ice and quickly add 1 mL of LB-broth. Incubate tubes for 1 hour on the 37°C shakers.
- Spread-plate 100 µL of cells on LB media containing the appropriate antibiotic. Alternatively, the cells can be diluted or concentrated before plating. For instance, centrifuge the tubes, pour off supernatant, resuspend cells in the final drop remaining in the tube and spread-plate the last 100 µL of cells.
- Incubate overnight at 37°C.
P.putida Competent Cells
Psudomonas electrocompetent cells are used for transformation of plasmid with or without the gene of interest by electroporation. Transformed Pseudomonas cells are grown at 30°C overnight on selective media to screen for insert.
- Inoculate 50mL LB broth with 1-7 days old Pseudomonas cells and incubate at 30°C in shaker overnight.
- Next morning, add entire culture to 500mL LB broth and check OD610.
- Incubate the culture at 30°C with shaking and check OD610 at 30mins intervals until it is 0.8~1.0.
- After transferring the cells into pre-chilled appropriate tubes, centrifuge at 7500g for 15mins at 4°C.
- Resuspend the pellet in 30mL cold electroporation buffer and centrifuge at top speed (~4500rpm or ~3000g) for 15mins at 4°C. (Note: 10%Glycerol can be used as electroporation buffer for Pseudomonas putida)
- Repeat step 5 for another two times.
- After centrifugation, resuspend pellet in 5mL cold electroporation buffer and place on ice.
- Pipette into multiple aliquots and store at -80°C.
E.coli Competent Cells
Competent bacterial cells are used for transformation of plasmids to allow propagation of plasmids and for expression of the genes of interest. Streak E.coli strain onto LB agar and incubate overnight at 37°C.
- Inoculate 5 mL LB broth with a single colony from LB plate. Incubate at 37°C with shaking.
- Measure OD600 of overnight culture. Aseptically inoculate 100 mL LB in 500 mL Schott Bottle with enough culture to obtain OD600 of ~ 0.05.
- Grow cells at 37°C, shaking until culture reaches OD600 of ~ 0.5.
- Aseptically transfer to centrifuge bottles and keep on ice.
- Harvest cells at 4000 rpm, 4°C for 10 min in cold centrifuge.
- Working in cold room with cells in ice, pour off supernatant and remove residual liquid by pipetting.
- Resuspend pellet gently in 33 mL RF1 solution. Mix by pipetting up and down.
- Incubate on wet ice for 1 hour.
- Pellet bacteria at 4000 rpm 4°C for 10 min.
- Again remove supernatant (work in cold room).
- Resuspend pellet in 8 mL RF2 solution and mix.
- Incubate on wet ice for 15 min.
- Working quickly, aliquot 110 µL of cell suspension into pre-chilled microcentrifuge tubes. Once dispensed, collect all tubes and store in -80°C freezer.
Transformation of P.putida bacteria via Electroporation
Electroporation, or electropermeabilization, is a molecular biology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, to allow DNA of interest to be introduced into electrocompetent Pseudomanas cells.
- Pre-chill electroporation cuvettes on ice for at least 10mins.
- On ice, aliquot 50uL of electrocompetent cells into microcentrifuge tubes.
- Add 1uL of appropriate purified plasmid for positive control; 2~3uL of ligation mix for test; and no DNA for negative control.
- Transfer mixtures into electroporation cuvettes.
- In "Exponential" protocol on the electroporator, change the settings to 2500 V, 25 µF, and 200 Ω.
- Place the dry cuvette in cuvette holder, press "Pulse" and note down time constant.
- Add 1mL LB broth to the cuvette and mix.
- Repeat step 6 and 7 for all samples.
- Aseptically pour the contents into respective new microcentrifuge tubes and incubate with shaking at 30°C for 1.5 hours.
- After incubating, plate the cultures on appropriate selective media plates in 100uL aliquots and as resuspended whole pellet.
- Incubate the plates at 30°C overnight and observe results.
IPTG Induction
- Grow the appropriate host by inoculating LB culture with the culture and incubating it while shaking
- After reaching an OD600 of ~0.1, add IPTG with final concentration of 1 mM
- Incubate for 3-4 hours while shaking
Note that the incubating temperature depends on the host.
Also, the same protocol can be followed by other inducers such as tetracycline (in low amounts) instead of using IPTG, however, the concentration in the inducing sample may be different.
NBP Assay
NBP or 4-(4-nitrobenzyl)pyridine assay is used to evaluate ethene monoxygenase activity. A chromophore is formed between ethene oxide and the nucleophile 4-(p-nitrobenzyl)pyridine (NBP) producing a violet colour.
- Add 2mL of MSM media (20mM K2HPO4, 5mM (NH4)2SO4, pH7) to plates containing culture of interest.
- Homogenise by scraping the cells and transfer to new eppendorf tubes.
- Centrifuge at 15,000g and resuspend in 200uL MSM media and take 10uL to check OD600.
- Repeat steps 1 to 3 with negative control.
- Transfer cell suspensions to 2mL autosampler vials and inject 200uL ethene gas.
- Incubate at 30°C overnight with shaking.
- Transfer suspension to microcentrifuge tubes and add 500uL of NBP reagent (100mM in ethylene glycol).
- Incubate at 95°C on heating block for an hour.
- Centrifuge at 13,000rpm for 5 minutes and transfer 500uL of supernatent to a new tube.
- Add 500uL of 1:1 (triethylamine: acetone) and observe for colour change and measure OD600 with deionized water as blank.
LB Media
LB media is a common growth media used for the propagation of bacterial cells.
- Mix 10 g Tryptone, 5 g Yeast extract, 5 g NaCl and 1 L of water, or use the same ratio in the required volume.
- For LB agar solution, add 15 g to the solution as well.
- Autoclave the solution to sterilise.
- Antibiotics can be added to make the media selective for antibiotic resistant cells. This must be added after autoclaving.
VIOGENE Gel/PCR DNA/RNA extraction kit
Working with DNA often involves adding enzymes (polymerases, restriction enzymes, ligases), which may subsequently need to be removed before they prevent or contaminate the next stage of work. DNA purification is quick and easy using a store-bought Kit.
Also consult the instruction booklet that comes with the Viogene kit – the protocol below only gives the bare essentials required. This kit purifies fragments of DNA (100bp – 10-kb) from agrose gel or other reactions with enzymes, dNTPs, salts and primers without the need for the phenol/chloroform extraction. The system is based on binding up to 20µg DNA to a silica-based membrane in chaotrophic salts with average recoveries of 60 to 90%.
Gel extraction -
- Use a clean, sharp scalpel or razor blade to excise the gel slice containing the DNA fragment of interest
- Measure the weight of the gel slice (about 50-200mg) and place it into a sterile 1.5ml or 2ml centrifuge tube. Add 0.5ml GP buffer.
- Incubate at 60°C for 5 to 10 minutes until the gel is competently dissolved. Invert the tube every 1-2 minutes during incubation
- Place a GPTM Column onto a Collection Tube and load no more than 0.6ml dissolved gel mixture into the column. Centrifuge for 30-60 seconds and discard the flow-through.
- Repeat step 4 for the rest of the mixture.
- Wash the column once with 0.5ml of WN Buffer by centrifuging for 30-60 seconds. Discard the flow through.
- Wash the column once with 0.5ml of WS Buffer by centrifuging for 30-60 seconds. Discard the flow through.
- Centrifuge the column at full speed for 3 minutes or more to remove residual ethanol
- Place the column onto a new 1.5ml centrifuge tube and add 15-20µl of Elution Buffer onto the center of the membrane.
- Stand the column for 2-3 minutes and centrifuge at full speed for 1-2 minutes to elute DNA. DNA can be stored at -20°C
PCR Purification –
- Pipet 10-100µl of PCR product or DNA solution after enzymatic reaction to a new 1.5ml centrifuge tube. Add 0.5ml GP Buffer and mix well
- Place a GPTM Column onto a Collection Tube. Add all the mixture from step 1 into the column
- Centrifuge for 30-60 seconds. Discard the flow-through
- Was the column once with o.5ml WN Buffer by centrifuging for 30 – 60 seconds. Discard the flow-through.
- Wash the column once with 0.5ml WS Buffer by centrifuging for 30-60 seconds. Discard the flow-through.
- Centrifuge the column at full speed for another 3 minutes or more to remove the residual ethanol.
- Place the column onto a new 1.5ml centrifuge tube. Add 15-30µl of Elution Buffer onto the center of the membrane.
- Stand the column for 2-3 minutes and centrifuge at full speed fro 1-2 minutes to elute DNA
- Store DNA at 4°C or -20°C
Bioline ISOLATE II Plasmid Mini kit
The miniprep was performed using the Bioline ISOLATE plasmid mini kit II and the protocol and more information about the kit can be found here.
Isolation of High Copy Plasmid DNA from e.coli
- Pellet 1-5 mL of a saturated E. coli culture for 30 s and collect pellet, removing as much supernatant as possible
- Add 250 uL of Resuspension buffer P1 and resuspend by vortexing/pipetting up and down
- Add 250 uL Lysis buffer P2, mixing by gently inverting 6-8 times
- After incubation for 5 minutes at room temperature, add 300 uL Neutralisation buffer P3, inverting 6-8 times
- Centrifuge the sample for 5 minutes and collect supernatant, taking care not to dissolve the pellet
- Place the supernatant - 750 uL at a time - into the spin column placed inside a collection tube
- Add 500 uL 50oC preheated Wash Buffer PW1 and centrifuge for 1 minutes, discarding flow through
- Add 600 uL Wash Buffer PW2 and centrifuge for 1 minutes, discarding flow through
- Centrifuge for another 2 minutes to remove residual ethanol
- Place spin column into a 1.5 mL microcentrifuge tube and add 50 uL Elution buffer directly on to the silica membrane
- After 1 minute incubation at room temperature, centrifuge for 1 minute.
Isolation of Low Copy Plasmid, P1 Constructs or Cosmid DNA from e.coli
- Pellet 5-10 mL of a saturated E. coli culture for 30 s and collect pellet, removing as much supernatant as possible
- Add 250 uL of Resuspension buffer P1 and resuspend by vortexing/pipetting up and down
- Add 250 uL Lysis buffer P2, mixing by gently inverting 6-8 times
- After incubation for 5 minutes at room temperature, add 300 uL Neutralisation buffer P3, inverting 6-8 times
- Centrifuge the sample for 10 minutes and collect supernatant, taking care not to dissolve the pellet
- Place the supernatant - 750 uL at a time - into the spin column placed inside a collection tube
- Add 500 uL 50oC preheated Wash Buffer PW1 and centrifuge for 1 minutes, discarding flow through
- Add 600 uL Wash Buffer PW2 and centrifuge for 1 minutes, discarding flow through
- Centrifuge for another 2 minutes to remove residual ethanol
- Place spin column into a 1.5 mL microcentrifuge tube and add 50 uL Elution buffer directly on to the silica membrane
- After 2 minutes of incubation at 70oC, centrifuge for 1 minute.
Note that centrifugations occur at 11000 x g.
Plasmid MidiPrep - 100 mL
This is the process used to extract plasmids from bacterial cells. Plasmids can then be used for screening or further manipulation.
- Pellet 100 mL culture in 2 x 50 mL Falcon tubes. Resuspend cells in 4 mL TE buffer, combine resuspended pellets in one tube.
- Add 8 mL lysis solution (SDS-OH), mix well by inversion and shaking(~10 sec). Should go viscous. Leave at room temp for 15 min.
- Add 6 mL ice-cold precipitation solution (K.Ac). Shake briefly – essential that K.Ac is thoroughly mixed in. Viscosity should disappear, and white precipitate appears. Keep on ice 15 min.
- Spin at top speed (4000 rpm) in cold Centaur centrifuge for 15 min. Recover tube immediately and handle gently (pellet is soft and easily resuspended). Pour supernatant into new tube. Try to avoid the white junk, but don't worry if little bits of it get transferred.
- Add an equal volume isopropanol (~15 mL), mix well ice 15 min.
- Spin at top speed (4000 rpm) in Centaur centrifuge (doesn't need to be cold) for 15 min, pour off supernatant, keep pellet.
- Add 10 mL 70% ethanol to pellet, resuspend by brief vortexing, leave for 5 min at room temp. Spin 15 min in Centaur centrifuge (doesn't need to be cold). Pour off supernatant again.
- Drain off excess supernatant by gentle tapping on paper towel, then heat at 50°C for approx 10 min to remove ethanol and isopropanol.
- Redissolve pellet in 2 mL TE with mixing (tapping tube ~ 1 min , don't vortex too much from this point onward). Can heat if necessary (eg. 50°C, 10-30 min). Note that plasmid DNA is much more soluble than chromosomal DNA, and dissolves preferentially. Solution should look slightly viscous (traces of chromosomal DNA still present).
- Split prep into 2 x 1 mL in Eppendorf tubes. Extract each tube with phenol: chloroform: isoamyl (PCI), as follows. Suck up 500 µL PCI from under the aqueous layer in the reagent bottle, transfer to Eppi tube. Vortex for ~5-10 sec until a uniform milky white emulsion is obtained. Centrifuge 5 min. Transfer top phase (aqueous) to a new Eppi tube. Discard bottom phase (PCI) into phenol waste. Avoid the white junk at the interface between phases.
- Repeat solvent extractions using 500 µl chloroform:isoamyl (CI, µl) per tube, as described for PCI above. After mixing & centrifugation, keep top aqueous phase, discard bottom CI phase into waste.
- Split DNA prep into 4 equally sized aliquots (~400 µl each) Precipitate DNA by adding 1/10-volume 3M Na-acetate (~40 µl) to each tube, and then add 2 volumes cold ethanol (~1 ml). Incubate >2 hr at -20°C (overnight is fine).
- Spin for 10 min, drain off supernatant, rinse pellet with 70% ethanol (as above, but using 500 µl 70% EtOH), drain excess EtOH off, then dry 10 min at 50°C.
- Redissolve plasmid in 100 µL EB. Expected yields range from approx 10 µg plasmid per ml culture (eg pUC/pGEM) down to 0.2 µg plasmid per mL culture (eg. RSF1010). Final expected DNA conc. may range from approx 10-500 ng/µL.
Solutions: (see Sambrook Appendix 1) [HARRY - WHAT?? do we have a reference to put in here?]
- TE (solution I): 10 mM Tris, 10 mM EDTA, pH 8. Autoclaved.
- Lysis sol'n (solution II): 0.2 M NaOH, 1% SDS. Prepare fresh from separate stocks (NaOH – 2 M, Autoclaved ; SDS – 10%)
- Precipitation sol'n (solution III): 3 M potassium, 5 M acetate, pH 4.8. Autoclaved.
- Na-acetate: 3M, pH 4.8 (adjust with conc. acetic acid), autoclaved.
- EB (Elution buffer): 5 mM Tris, pH 8. Autoclaved.
NOTE: RNAse can be added to TE buffer at the start, or to the EB/TE at the end. RNA doesn't interfere with most things, but can make gels look messy and obscure small DNA bands. Add RNase from conc., boiled stock (10 mg/ml) to final conc. of ~100 µg/ml.


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The TRILOGY
I have been getting so many enquiries from girls who are not yet Seniors or have already graduated about shooting with me that I wanted to come up with an open to all shoot concept that shows off what I love to do in the photography business. I wanted to do something a little bit different than the ordinary so I came up with the Trilogy.
The TRILOGY is three photo shoots spread over three weeks with three girls in each group. The girls would model in all three shoots and stay with the same girls through the whole concept. So if they wanted to sign up with a couple of friends they could enjoy an experience together or they could sign up on their own and make some new friends.
The three shoots I wanted to do were a night shoot, a beach shoot and a swimming pool shoot. I love all three of these shoots and I thought it would be fun to add them all in one.
I have shot two of the four groups of girls so far and it could not be more fun!
Keywords: baton rouge, baton rouge senior photographer, baton rouge senior photography, denham springs, denham springs photographer, high school senior photos, zerofourfour photography
No comments posted.
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A 40-year – old electrical engineer, Enyinnaya Ukpabi , in Amenu Edem -Ani community of Nsukka Local Government Area was electrocuted to death on the February 23rd
Mr Ebere Amaraizu , spokesman of Enugu state police command said in a statement
" It was gathered that the deceased , a retired electricity officer, was said to be clearing fault from a public power supply line, In the course of the clearing of the fault , there was a sudden trip – off on the line , leading to his electrocution" .
" He was promptly rushed by sympathisers to a private hospital , where he was later confirmed dead . "


text: Although Unite international industrial and solidarity activities are carried out by the International department, officials and lay activists in all levels of the organisation, overall political guidance and direction is carried out by the International Committee of the Unite Executive Council. This Committee was established in March 2011 and consists of five lay members of the Executive Council. Underlining the importance of this Committee the vice chair of the Executive Council Mark Lyon is chair of the International Committee. All the Committee members have significant international experience in their own right from ITF to IndustriAll and have also been considerably involved in Unite's solidarity work including Cuba, Columbia, Palestine and South Africa. Mark Lyon – chair Executive Council member for Chemicals pharmaceuticals, process and textiles sector and Vice chair of Unite Executive Council Dave Williams Executive Council member for Road Transport commercial sector Dennis Wilson Executive Council member for Food, Drink and Tobacco sector Glenn Jackson Executive Council member for Graphical, Paper and Media sector Pat Stuart Executive Council member for Community, Youth workers and Not-for-profit sector


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We wanna say thank you Darinimages for Pre Wedding Photos Session. You make me and Lookpear so happy with those wonderful pics, we can't stop seeing them. I'm so amazed how you can find the place for us to do a photoshoot, somewhere I have no idea how you can make it until I saw your pics. WOW!!!! IT'S AWESOME.
We are really appreciated to work with you. Thanks for your hard working with us na krub.
Darin, You are incredible.
Ching & Pear,
Bangkok, Thailand


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Flames swimmers destroy competition at ODU Invitational
September 30, 2017 | Norfolk, Va.
Liberty University's men's swimming & diving debut was a tremendous success, Saturday at the Old Dominion University Fall Invitational. The Flames finished first out of six men's teams with 707.5 points, leaving William & Mary (460) and ODU (360) in their wakes. Virginia Commonwealth (276.5), Georgetown (239.5), and Christopher Newport University (228.5) rounded out the field.
"We crushed it," Flames Head Coach Parker Spencer said. "It was good to get some good competition against some big schools. The guys performed like we thought they would and it was really good for the swimmers to see all of their hard work pay off on their first trip and competition together as a team."
Liberty originally scheduled to open its season at a meet hosted by Appalachian State, but had to change its plans when that meet was overbooked.
"It was really good for our guys to get in and get a meet under our belt," Spencer said. "The season started out just the way we wanted it to."
Spencer was amused by overhearing several swimmers and spectators say, "I didn't even know Liberty had a swim program," he said. "Almost every event we entered we won and a majority of the team qualified for (USA Masters Swimming collegiate) nationals. It was a really good day."
The Flames posted more than 20 national qualifying times, based on standards set by the Collegiate Club Swimming Association under USA Masters Swimming.
Freshman Joseph Hall won all but one event he competed in, including the 50-yard freestyle in 21.72 seconds and 200 free in 1:50.78. He placed second behind William & Mary's Kyle Smith in the 200 individual medley (IM) in 2:05.24. Hall anchored the Flames' winning 200 free relay (1:30.13) and 200 medley relay (1:41.46). Ethan Raub, another freshman from North Carolina, also swam legs on both winning relays and won the 100-yard butterfly (53.92) before finishing second in the 50 fly (24.96).
Sophomore Thomas Sonnery-Cottet, a standout on the Flames' triathlon team, demonstrated his endurance in the pool, winning the 500 free by nearly five seconds in 5:01.65 and also placed third in the 200 free in 1:53.37. Jay Hutchinson, a freshman from Tampa, Fla., won the 100 IM in 58.09.
Freshman David Schafer placed second in the 50 breast stroke in 29.29. Cameron Foster finished third in the 100 backstroke (1:00.92) and fourth in the 50 back (27.80). Adam Williams placed fourth in the 50 fly (25.71) and fifth behind Team USA triathlete Bobby Hammond in both the 200 free (1:57.97) and 500 free (5:30.32). (Hammond finished fourth in 1:57.68 and 5:23.40, respectively).
Complete results are available online.
Liberty's next meet is set for Oct. 20-21 at Coastal Carolina in Wilmington, N.C., where the host Chanticleers will be among the favorites along with defending USA Masters Swimming national champion Virginia.
Ben Davidson, a freshmen sprint freestyler from Forest, Va., should help the Flames in that competition after missing this past weekend's meet.
The Flames will then travel to the Raleigh (N.C.) Invitational, one of the biggest club-level meets on the East Coast, hosted by North Carolina State on Nov. 11.


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Ridgeline Lever Button 135 PAIR
Ridgeline poles are ideal for fast and light expeditions, multi-day backpacking trips, or travel. Lightweight and stiff, they offer stability for those carrying heavy packs, or those looking for a little more strength out of a lightweight pole. The extended-length hand grip offers variation on holding position, and additional adjustability can be achieved by the lever lock. The Lever Lock with button model makes you the collapsed length you would normally only find in a three-section pole possible with the adjustability of a two-section pole. A fixed-length lower shaft that doesn't require adjustment keeps you fast on the go.
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Take great delight from organic forms in lovely hues kissed with gold.
One bowl in a gift box
Returns are accepted for full credit up to 14 days from delivery date. Shipping charges are not refundable. See our Shipping & Returns Page for more details
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Christopher Williams was born in Pensacola, Florida in 1973. He writes Fantasy, Historical Espionage, and Supernatural Thrillers.
Chris graduated from the University of Florida with a degree in Electrical Engineering, but his love is writing. He hopes to one day be able to write full-time.
Chris' website is http://BooksbyChristopherWilliams.com
Review by:
Andre Marks
on Dec. 21, 2010 :
(no rating)
excellent writting form. well thoughtout and but together
(reviewed 10 months after purchase)


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Bowling Ball Stickers
More Bowling Ball Stickers from Zazzle
These cute stickers have both a bowling ball and pins and come in some cool colors like hot pink. Your bowling league will delight in seeing their name on these unique stickers. They also add a little pizazz to a name tag!
Leave a Reply.
Retro Invitations, Gifts & Party Ideas


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MegaTen launches Chain of Curse
Megaten (Shin Megami Tensei: Imagine Online) has announced the launch of a brand new update, "Chain of Curse."
This first episode of "Chain of Curse" is a story written by one of the original creators of the Shin Megami Tensei franchise. Players will lead Demon Busters through brand new areas, meeting new characters through new quests in the storyline including three new LAW acts and a new boss. New in-game events and game changes have also been implemented in the update.
Megaten Gameplay Screenshot
The Original Press Release:
Aeria Opens the Next Chapter of Shin Megami Tensei: Imagine Online
Chain of Curse Arrives in Celebrated Free-to-Play MMORPG
SANTA CLARA, Calif. – July 21, 2010 – Aeria Games, a leading publisher of interactive entertainment, has added the next chapter to Shin Megami Tensei: Imagine Online ("MegaTen"). Arriving today in the celebrated MMORPG via a free content update, MegaTen's immersive story now includes the first episode of "Chain of Curse," a long-awaited tale written by one of the franchise's original creators.
Episode One of Chain of Curse will challenge players to lead their Demon Busters through new areas, meeting new characters and completing new quests as they explore an intriguing new mystery. The new content update will also provide new in-game events, and a selection of game changes designed specifically around community requests.
For more details about Shin Megami Tensei: Imagine Online, and to sign up for a free game account, please visit http://megaten.aeriagames.com.
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Find the perfect place from the worlds best selection of vacation rentals on HomeAway.
Our 2018 property listings offer a large selection of 252 vacation rentals near Robe. From 221 houses to 16 cottages, find a unique house rental for you to enjoy a memorable stay with your family and friends. The best places to stay near Robe for a holiday or a weekend are on HomeAway.
HomeAway offers a large selection of vacation rentals near lovely places in Robe. From HomeAway travelers, the most popular points of interest to stay for a holiday trip or just for a weekend are:
But there are many other points of interest around Robe listed on HomeAway where you can find your place to stay. Please use our search bar to access the selection of vacation rentals available near the point of interest of your choice.
Yes, of course. HomeAway has 221 houses in Robe. Our other popular types of vacation rentals in Robe include:
Absolutely, HomeAway has 112 golf resort rentals in Robe. You have also many other possibilities in Robe area, including:
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Yes, you can select your preferred vacation rental with pool among our 3 vacation rentals with pool available in Robe. Please use our search bar to access the selection of vacation rentals available.
The ocean and lake provide a pleasant backdrop to your vacation home in Robe. Travelers especially like the spas, camping and seafood in this welcoming and relaxing city. Need more space to accommodate a family vacation, friends trip or romantic retreat? Rental homes often come with a full kitchen, separate dining area and laundry facilities.
Looking for things to do while you're in Robe? To appreciate the outdoor scene, make time for beaches like Long Beach and green spaces such as Little Dip Conservation Park.
Robe Golf Club and Obelisk Robe are some attractions to visit during your stay. Also you might want to add Cape Thomas Wines, Wehl's Mount Benson or Frog Island Wines to your agenda.


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K Way Kids
reversible padded jacket
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New! Order by phone
Ensure your little ones stay stylishly dry with K-Way Kid's ultra-lightweight and easy to carry packable designs. This grey and yellow feather and duck feathers reversible padded jacket features a hood, a front zip fastening, front zipped pockets, a logo to the chest, long sleeves, elasticated cuffs and a high low curved hem.
Designer colour: 978
Made in Italy
Designer Style ID: K001K40
Farfetch ID: 12320657
- Lining Composition
- Polyamide 100%
- Outer Composition
- Polyamide 100%
- Lining Composition
- Duck Feathers 90%
- Lining Composition
- Feather 100%
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Import duties information
Delivery duties are included in the item price when shipping to all EU countries plus USA, Canada, China, Australia, Puerto Rico, Switzerland, Singapore, Republic Of Korea, Kuwait, Mexico, Qatar, India, Norway, Saudi Arabia, Taiwan, Thailand, U.A.E., Japan. This means all import duties are included in your order. The price you see is the price you pay.
Delivery duties are not included in the item price when shipping to all other countries. This means you may be liable to pay import duties on receipt of your order.


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University Art Gallery
The University Art Gallery serves as a showcase for the visual arts in the border region. The largest visual arts facility in South Central New Mexico, the gallery presents six to nine exhibitions annually. While the gallery has a permanent collection, it also focuses on the works of the university art department's own faculty, graduate students and undergraduate students. Go there >>
Zuhl Library Collection
Part art gallery, part natural history museum, the Zuhl collection at NMSU showcases thousands of beautiful specimens of petrified wood, fossils and minerals. The majority of the pieces can be viewed at the Zuhl Museum but don't miss the displays in the Zuhl Library. You may also access our database to search by category and/or display location. Go there>>
University Museum
Located in Kent Hall on the corner of University Avenue and Solano Drive, the University Museum has provided 50 years of educational service while giving the community a resource for cultural displays. Go there >>


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9IWGTD, Adelaide, AGWA, australia, disease, grapegrower, grapevine, International Workshop on Grapevine Trunk Diseases, National Wine Centre, SARDI, trunk, University of Adelaide, vineyard, wine, winemaker, workshop
GRAPEGROWERS and winemakers have an opportunity to learn the latest information and practical management options for grapevine trunk disease.
The 9th International Workshop on Grapevine Trunk Diseases (9IWGTD), sponsored by the Australian Grape and Wine Authority (AGWA), is being held in Australia for the first time and will showcase the Australian wine industry's proactive methods for trunk disease management.
Hosted by the South Australian Research and Development Institute (SARDI) and the University of Adelaide, the workshop will be held at the National Wine Centre in Adelaide from November 18-20 and is being convened by Dr Mark Sosnowski (SARDI).
The third day of the conference is an industry forum day, and Australian grapegrowers and winemakers have been offered a discounted fee to attend it on November 20 through AGWA's sponsorship.
AGWA's acting chief executive officer Andreas Clark said the industry forum day presents a rare opportunity for industry members to learn the latest practical management options for trunk diseases from Australian and international experts.
Dr Sosnowski said he "strongly encourages Australian industry representatives to attend the industry forum day, which will focus on the latest information from around the world on practical management options in the vineyard and nursery".
Industry forum day registration for Australian grapegrowers and winemakers is subsidised to $200, if their registration is received by August 17.
Industry members are also welcome to register for other days if they wish.
Registration details are available from www.plevin.com.au/iwgtd2014/registration.html.
Contact: Mark Sosnowski


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Stunt Performers from "Unbroken" and "Game of Thrones" are Honored by SAG-AFTRA for Outstanding Action Performances by Film and Television Stunt Ensembles
LOS ANGELES (Jan. 25, 2015) — The stunt performers and coordinators from "Unbroken" and "Game of Thrones" are this year's SAG Awards honorees for Outstanding Action Performances by Film and Television Stunt Ensembles, it was announced today from the 21st Annual Screen Actors Guild Awards® red carpet by Screen Actors Guild Awards Committee Chair JoBeth Williams and Vice Chair Daryl Anderson. The announcement was made on the live "21st Annual SAG Awards Red Carpet Show," and was webcast on sagawards.tntdrama.com, sagawards.org and PEOPLE.com on Sunday, Jan. 25, beginning at 6 p.m. (ET) / 3 p.m. (PT), with the Stunt Ensemble recipients announced at 6:15 p.m. (ET) / 3:15 p.m. (PT).
The Stunt Ensemble honors commend work within the stunt community during 2014 and recognize stunt performers and coordinators. Nominees chosen by their respective SAG Awards film and television nominating committees were announced on Dec. 10, 2014. Information on voting procedures to choose the recipients was sent to the 111,228 active voting members of SAG-AFTRA on Dec. 16. Balloting closed on Friday, Jan. 23 at noon (PT). Integrity Voting Systems, the Awards' official election teller, sealed the results until Williams and Anderson announced them on the SAG Awards red carpet.
The recipients for this year's Stunt Ensemble honors are:
Unbroken (Universal Pictures)
Lee Adamson
Joel Amos Byrnes
Richard Anderson
Yoshianao Aonuma
Glenn Boswell
Jimmy Christiansen
Simon Crane
Haydn Dalton
Ahsley Fairfield
Toby Fuller
Scott Gregory
Brendan Jakins
Alex Jewson
Chris Mitchell
Craig Morgan
Chris Patton
George Saliba
Mick Saliba
Nik Schodel
Brett Sheerin
Neil Stoddardt
Leon Stripp
Ben Toyer
Nick Turello
Darko Tuskan
Bernadette van Gyen
Gavin Wilde
Darren Yost
Game of Thrones (HBO)
Richard Bradshaw
Jonathan Cohen
Jason Curle
David Foreman
Dean Forster
Paul Herbert
Paul Howell
Stewart James
George Kirby
Guy List
Daniel Naprous
Andy Pilgrim
CC Smiff
Shane Steyn
Richard J Wheeldon
NOTE: Credits for the Stunt Ensemble Nominees are not final. All names are subject to verification.
About the 21st Annual Screen Actors Guild Awards®
The 21st Annual Screen Actors Guild Awards® presented by SAG-AFTRA with Screen Actors Guild Awards, LLC will be produced by Avalon Harbor Entertainment, Inc. and will be simulcast live on TNT and TBS on Sunday, Jan. 25, 2015 at 8 p.m. (ET) / 5 p.m. (PT). A primetime encore presentation will follow immediately on TNT. The SAG Awards can also be viewed live on the TNT and TBS websites, and also the Watch TNT and Watch TBS apps for iOS or Android. (Viewers must sign in using their TV service provider user name and password)
Prior to the televised ceremony, honorees for outstanding television and film stunt ensemble action performances will be announced from the red carpet during the SAG Awards Red Carpet Pre-show, which will be webcast live on sagawards.tntdrama.com, sagawards.org and People.com beginning at 6 p.m. (ET) / 3 p.m. (PT).
One of the awards season's premier events, the SAG Awards annually celebrates the outstanding motion picture and television performances from the previous calendar year. Of the top industry honors presented to actors, only the SAG Awards are selected entirely by 111,228 performers' peers in SAG-AFTRA. The SAG Awards was the first televised awards show to acknowledge the work of union members and the first to present awards to motion picture casts and television ensembles. For more information about the SAG Awards®, SAG-AFTRA, TNT and TBS, visit sagawards.org/about.
Photos and graphics for the 21st Annual Screen Actors Guild Awards are available at sagawards.org/media-pr/photos-and-artwork.
Connect with the SAG Awards®
Hashtag: #sagawards
Website: http://sagawards.org
SAG Awards Red Carpet Pre-Show: http://www.sagawards.tntdrama.com
Facebook: http://www.facebook.com/sagawardsofficialpage/
Twitter: http://twitter.com/sagawards/
Google +: http://google.com/+SAGawards/
Instagram: http://www.instagram.com/sagawards/
Tumblr: http://sagawards.tumblr.com
Blog: http://blog.sagawards.org


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SYMPTOMS: Shortness of breath and difficulty performing any kind of physical activity are the most common symptoms of emphysema. Loss of appetite and weight loss is another common symptom those with emphysema experience. Eating can become a tiring activity resulting in loss of breath. In addition to feeling short of breath, a full stomach pushes up on the diaphragm making it harder to breath. As a result, eating becomes less desirable. Fatigue is also a symptom of emphysema. Overtime shortness of breath and decreased oxygen levels will deprive a person of energy. Coughing is uncommon for patients with emphysema and when they do cough it is unproductive, meaning little to no phlegm comes up.
TRADITIONAL TREATMENTS: The most important treatment step for those with emphysema who still smoke is quitting. Nicotine replacement methods and medication can help curb cravings that typically occur for the first few weeks after quitting. Bronchodilator drugs and inhaled steroids help relieve symptoms of emphysema by opening up air passages. Supplemental oxygen is also used to provide relief from emphysema. Surgery is also an option for acute emphysema suffers. Lung volume reduction surgery removed wedges of damaged lung tissue so that patients can breath more efficiently. When all other options have failed, lung transplant surgery is done in extreme cases.
NEW TREATMENT OPTION: A new surgical procedure is currently being studied that may relieve emphysema patients from constantly feeling short of breath. During the procedure, the surgeon inserts a flexible bronchoscope down the patient's mouth and into the airways. A Doppler probe is then inserted through the bronchoscope to identify places in the airways where small holes can be made. The holes must be made away from blood vessels and are made using a special needle. Once up to six holes have been made, stents are then placed in the holes to keep them from closing. The surgery takes about one to two hours and, so far, patients report noticing an immediate improvement in their shortness of breath.
FOR MORE INFORMATION, PLEASE CONTACT: EASE Stent Trials: Bronchus Technologies, Inc. / http://www.EASEtrialUS.com / (866) 431-3273


text: STOCK BUY-BACK. The board of Martin Marietta Corp. has authorized repurchase of up to 3 million shares of the company's common stock, or 5 percent of about 58 million shares outstanding. Goldman, Sachs & Co. will act as exclusive agent for the repurchase program, which does not have a time limit. Martin Marietta, based in Bethesda, Md., is an aerospace and technology company that produces systems, products and services in the fields of space, defense and information management. It is the parent of Martin Marietta Orlando Aerospace.


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Acg Coll Works Forbidden Worlds HC Vol. 04
- Publisher: PS Artbooks
- Category: Graphic Novels
- Retail: $47.99
- Release: 2013-09-25
They may have been Forbidden to some, but to kids the length and breadth of the United States (and a select few in the UK), Richard Hughes and his tremblin' team of terrific tormenters were where it was at. Big time. Tempus certainly does fugit. Doesn't seem like five minutes since we were putting the finishing touches to the inaugural volume of our Forbidden Worlds hardcover reissue series and already we're on the third book. Stick with the programme, gang-things are starting to get real interesting! Reprints issues #19-25 from July 1953 to January 1954.