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Chromodomain Helicase Binding Protein 8 (Chd8) is a Novel A-kinase Anchoring Protein Expressed During Rat Cardiac Development
Publication Title
PloS One
Publication Date
Document Type
immunoprecipitation, immunostaining, fluorescence microscopy, HeLa cells, immunofluorescence, phosphorylation, muscle cells, heart
A-kinase anchoring proteins (AKAPs) bind the regulatory subunits of protein kinase A (PKA) and localize the holoenzyme to discrete signaling microdomains in multiple subcellular compartments. Despite emerging evidence for a nuclear pool of PKA that rapidly responds to activation of the PKA signaling cascade, only a few AKAPs have been identified that localize to the nucleus. Here we show a PKA-binding domain in the amino terminus of Chd8, and demonstrate subcellular colocalization of Chd8 with RII. RII overlay and immunoprecipitation assays demonstrate binding between Chd8-S and RIIα. Binding is abrogated upon dephosphorylation of RIIα. By immunofluorescence, we identified nuclear and perinuclear pools of Chd8 in HeLa cells and rat neonatal cardiomyocytes. We also show high levels of Chd8 mRNA in RNA extracted from post-natal rat hearts. These data add Chd8 to the short list of known nuclear AKAPs, and implicate a function for Chd8 in post-natal rat cardiac development.
Recommended Citation
Shanks, Maureen O.; Lund, Linda M.; Manni, Sabrina; Russell, Mary; Mauban, Joseph R. H.; and Bond, Meredith (2012). Chromodomain Helicase Binding Protein 8 (Chd8) is a Novel A-kinase Anchoring Protein Expressed During Rat Cardiac Development. PloS One 7(10), 1-14. doi: 10.1371/journal.pone.0046316 Retrieved from https://digitalcommons.kent.edu/bscipubs/129

subset: commoncrawl


text:
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|Donald Ball <ba...@webslingerZ.com>
|Re: Sitemap and Links...
|Thu, 06 Jan 2000 20:07:37 GMT
On Wed, 5 Jan 2000, Stefano Mazzocchi wrote: > > I'm thinking in the > > case of most hosting companies, which offer plans with a lot of cool stuff > > (cgi, php, mysql, or even coldfusion...) but without having a dedicated > > server for each costumer. Are ISP going to be able to offer Cocoon as a > > extra service without too much pain? > > Yes, IMO, much more easier to leave a single Cocoon for each virtual > host rather than having one cocoon for all. A lot more client > flexibility of configuration. Okay, I must bring up a possible counterexample. Some of my designers feel that 'hostname' directed browsing is going to become more and more prevalent. that is to say, instead of URLs like this: http://news.com/europe/ http://news.com/africa/ we'll use things like this: http://europe.news.com/ http://africa.news.com/ That's all well and good, but it's very likely that one would want to use the same sitemap for all *.news.com sites, just direct the user towards different XML data (and possible stylesheets) depending on the hostname chosen. Now, there's nothing in the servlet spec that prevents many virtual hosts from sharing one servlet. Apache-JServ allows one servlet zone to be mounted in many virtual hosts. Why can't we build something into the sitemap to accomodate site designers who want this sort of functionality? <host name="europe.news.com"> ... other directives here ... </host> Next question - it would seem that the general consensus is towards Pier and Stefano's proposed map syntax, with the 'prefix' tag as suggested by Brett. Is that so? If so, let's work on a pseudocode algorithm for using the sitemap and then implement it already. I can't see anything horrendously difficult there (not anything as hard as, say, XSP :)) - donald
|View raw message


text:
Let's face it: the stress of planning a wedding can weigh down even the best of us. If you've been feeling like a ball of nerves lately, yoga might be the key to relaxing and resetting your mind for the road ahead. And there's no better place than the idyllic island of Bali for going 'om' and finding your zen. Here, we've picked out yoga retreats in Bali that'll help you beat any pre-wedding stress.
Uluwatu Surf Villas, Uluwatu
Perched over the Indian Ocean in the middle of Bali's surfing mecca is our favourite yoga location in Bali. The Morning Light yoga studio at Uluwatu Surf Villas is tucked away amongst lush gardens and private villas, with the ocean rumbling beneath you. It features antique ironwood beams and polished reclaimed teak floors shaded by a traditional thatched grass roof, creating an open-air studio at one with the ocean breeze and sounds of the surf. The estate's location on the South peninsula usually guarantees sunny days, tropical breezes, and ultra consistent surf so you can stretch and surf until your heart's content. With three classes daily all open to the public, it's too easy to fit in a surf, swim and sleep around your morning or sunset yoga sessions.
Uluwatu Surf Villas Jalan Pantai Suluban Uluwatu, Pecatu, 80364 Bali, p. +62 0817 555 365.
The Yoga Barn, Ubud
This is where Ubud's yoga pulse beats: a number one stop for anyone travelling to Bali with a yoga mat within arms reach. Yoga Barn is spectacular two storey construction made of wood and bamboo overlooks the iconic Balinese surrounds – swaying palm trees and emerald green, luscious rice fields. Classes here vary from classic Hatha to more exotic Embodied Flow, Yoga Nidra, Laya Yoga and other activities like Tibetan Bowl Meditation, Sound Medicine and a community favourite – Ecstatic Dance, where everyone is welcome. Rub shoulders with the crème de la crème of Ubud's yoga community and dance like there is no tomorrow. Practicing the art of 'enjoying the moment', has never been more fun.
Yoga Barn, Jalan Raya Pengosekan Ubud, Ubud, Bali 80571, p. +62 361 971236.
Desa Seni, Canggu
Desa Seni is an eco-friendly hotel and a Canggu institution that's more of a community playground than just a pretty tourist accommodation. The beautiful and amazingly well-groomed estate houses bountiful vegetable gardens, a salt water pool, and two spacious yoga pavilions set in antique joglos. No walls, no troubles – let the frangipani-scented breeze cool you down while the best yoga teachers in Bali guide you through the practice. Don't be surprised if one of the local dogs happily walks in during shavasana time, and curls right next to you.
Desa Seni, Jalan Subak Sari No.13, Canggu, Bali 80361, p. +62 361 8446392.
The Power Of Now Oasis, Sanur
At the quiet wave-free beach of Sanur with swaying palm trees and the gentle breeze – what more can a yoga-lover ask for? The spacious two-story, open-plan shala of The Power Of Now Oasis is made entirely of bamboo and hosts a studio, teahouse, organic juice bar, library, chill-out area, and plenty of space to chat about your doshas and chakras with like-minded folks. Though this centre is bliss and relaxation personified, it is a yoga powerhouse of all you would love to experience, and more.
The Power Of Now Oasis, Jalan Mertasari, Denpasar Sel., Kota Denpasar, Bali 80224, p. +62 361 288 833.
Four Seasons Sayan, Ubud
A wooden terrace in the middle of blooming lotus pond, floating high above the misty jungle valley – that's what one of world's most beautiful yoga spot looks like and this one is nestled in the Four Seasons Sayan resort in Ubud. These sunrise classes are worth skipping the wine testing the night before and waking up at dawn. It's too bad this privilege is available for hotel residents only, though it's definitely worth the splurge to treat yourself.
Four Seasons Sayan, Jl. Raya Sayan, Ubud, Bali 80571, p. +62 361 977 577.
Samadi, Canggu
A community-oriented space that has everything to please a New Age warrior: Samadi is a weekly farmers market, vegetarian café, and elegant yoga space in Canggu with a terrace that's equipped with a cocoon swing! Designed by a Dutch architect, this sleek studio is an oasis of contemporary cool surrounded by tropical greens and koi carp pools.
Samadi, Jalan Padang Linjong, Kuta Utara, Kab. Badung, Bali 80351, p. +62 812 3831 2505.
Samanvaya, Sidemen
Looking for a real hideaway? You've just arrived. Sidemen, located on the East coast, half way to Candidasa, is worth running away to. It's a magical land of untouched greenery, where farming is the only social activity. No shops, no street vendors, no surfers around – pretty much nothing at all, just an absolute serenity. The yoga pavilion at Samanvaya boutique retreat is a massive bamboo construction standing tall above endless rice paddies and inviting for the deep, undistracted practice. Try your best not to nap when in meditation!
Samanvaya, Br. Tabola, Sidemen, 80846, Karangasem, Bali, Indonesia, p. +62 82 147 103 884.
This article was contributed by Masha Mozolevskaya, travel and lifestyle journalist, and founder of the Canggu Chronicles blog.


text:
Midland Midwives Nottawasaga
at CSC Chigamik CHC
845 King Street, Unit 10
Midland, ON L4R 0B7
Tel: 705.527.4154
Toll Free: 1.855.527.4154
Fax: 705.526.2870
FILL OUT AN INTAKE FORM and we will contact you with an appointment.
Clients are asked to fill in an intake form for the clinic where they would like to receive their prenatal and postpartum care.
The Collingwood Clinic operates out of its location on Beachwood Road, and the midwives there offer births at home or at the Collingwood General and Marine Hospital (CGMH).
The Midland Clinic operates out of the Chigamik Community Health Centre, and the midwives there offer births at home, Georgian Bay General Hospital (GBGH - Midland) and Orillia Soldiers' Memorial Hospital (OSMH)
Drop By and Visit
Drop By and Visit
Collingwood Midwives Nottawasaga
9811A Beachwood Road
Collingwood, Ontario
705 446 2511
FILL OUT AN INTAKE FORM and we will contact you with an appointment.


text:
The SCCG received funding from the Office of Emergency Management under the State Emergency Management Projects Program to continue Stage 2 of the Emergency Management Health Check.
In late 2015 SCCG commenced the state wide project to enhance, implement and embed the Emergency Management Health Check into local government processes and frameworks.
The project objectives are to:
A Project Advisory Committee was set up to support and advise on project deliverables; including representatives from the Office of Emergency Management, local councils, state government and Universities.
SCCG engaged consulting firm Janellis to functionalise the tool, facilitate stakeholder workshops and to develop a resource package for local councils.
View the project factsheet here.
Stakeholder engagement workshops were undertaken across NSW to raise awareness and gain feedback and input into the draft tool, particularly the questions framework. Workshops were held at North Sydney, Newcastle, Dubbo, Ballina, Ulladulla and Lidcombe.
Workshops were open to all Councils including staff involved in emergency management, risk management, business continuity planning, governance, environment, asset management and community services. The Regional Emergency Management Officers and representatives of the combat agencies were also invited to provide feedback.
As a component of the project an online 'Health Check Tool' and an
online resource toolkit have been developed and can be accessed from the project website. This website is now live - go to: www.emhealthcheck.com.au
The Tool will enable councils to assess their own level of performance 'health' in relation to emergency management planning, preparedness, response, recovery and overall resilience.
All NSW councils are encouraged to complete the Health Check Tool prior to June 2017.


text:
Ned McCrory will be speaking at the CMAA Carolinas Chapter Meeting
Event Date: September 20, 2016
Ned McCrory, Managing Partner and Head of the Private Club Practice Group, will be speaking at the CMAA Carolinas Chapter Meeting in High Point, North Carolina. The Club Managers Association of America (CMAA) is the professional association for managers of membership clubs. With close to 7,000 members across all classifications, CMAA's manager members run more than 3,000 country, golf, city, athletic, faculty, yacht, town and military clubs. The Carolinas Chapter is comprised of more than 350 members within North and South Carolina. Ned's presentation is titled "Seeing your Clubs financial statements through the eyes of your banker."


text:
Find us on Facebook | Follow us on Twitter
The 2018 M&M Summit will be held at the University Plaza Hotel and Springfield Expo Center in Springfield, MO on July 16-18.
Hotel Reservations
The University Plaza Hotel
Be sure to mention you are with
the General Baptist Conference
Be sure to let the reservationist know you are with the General Baptist convention.
- Looking to partner with General Baptists through exhibiting at the Summit or through a Sponsorship with General Baptist Ministries? Check out our Sponsorship Page.