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Mount Prospect, IL — In 2008, the number of people who worked out at a club increased to 39.3 million people, according to the National Sporting Goods Association (NSGA). That made working out at a club the No. 8 participant activity in NSGA's annual "Sports Participation — Series I and II" reports. In 2007, the survey ranked working out at a club at No. 10 with 33.8 million participants.
The data also showed that exercise walking, which experienced 7.6 percent growth in 2008, remains the No. 1 participant activity, a position it has held since 1990. In 2008, 96.6 million Americans walked for exercise. In 2007, exercise walking had 89.8 million participants.
Swimming, with a 6.1 percent increase, moved ahead of exercising with equipment for the No. 2 spot. With its increase, swimming attracted 63.5 million participants. Exercising with equipment attracted 63 million participants, placing it in the No. 3 position. Last year's survey showed that 52.8 million participants exercised with equipment.
Bowling claimed 49.5 million participants for the No. 4 spot. Camping (vacation/overnight) claimed the No. 5 spot with 49.4 million participants.
New to the Top 10 was hiking with 38 million participants. This pulled it to the No. 9 position. Falling from the Top 10 was power boating, which dropped to No. 15 after a 13 percent decline in participation (27.8 million participants).
Rounding out the Top 10 were bicycle riding, No. 6 with 44.7 million participants; fishing, No. 7 with 42.2 million participants; and weight lifting, No. 10 with 37.5 million participants.
For the survey, a participant is someone age 7 or older who takes part in a sport or activity more than once in a calendar year. "Sports Participation in 2008 — Series I and II" cover 41 sports, recreation and fitness activities. Participation rates for all 41 sports surveyed may be viewed on NSGA's Web site at www.nsga.org.


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18 families displaced in Greensboro apartment complex fire
18 families displaced in Greensboro apartment complex fire
An apartment complex fire displaced 18 families from their homes in Greensboro on Monday night, fire officials said.
The fire at 429 Guilford College Road started shortly before 9 p.m. Monday, according to the Greensboro Fire Department.
About 55 firefighters and 25 fire trucks responded to the fire that prompted the evacuation of 18 units in the complex, Greensboro Fire Department Deputy Chief Hunter said.
Two of the units sustained heavy damage. Hunter said 25 adults and 10 children were displaced by the fire. The Red Cross is assisting the victims.
The fire was extinguished about 45 minutes after firefighters arrived, officials said.
No injuries were reported in the incident. The cause of the fire was under investigation.

subset: commoncrawl


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Three-time reigning Olympic champion Team USA starts group play against New Zealand in women's soccer.
Host country Brazil plays its first men's soccer match against South Africa.
Will Pelé light the torch at the opening ceremonies?
Team USA starts play against China in men's basketball. Three-time Olympic gold medalist Kerri Walsh Jennings has a new partner (April Ross) in beach volleyball. Qualifying begin for men's gymnastics.
Sunday, Aug. 7
Keep an eye out for swimmer Katie Ledecky, the world-record holder,...


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OKLAHOMA CITY—Freehold, N.J.-based Monmouth Real Estate Investment Corporation has purchased a 119,912-sq.-ft. industrial building located at 2710 South West 18th Street in Oklahoma City, Okla. for $9.453 million. The property is net-leased for 10 years to FedEx Ground Package System Inc.
Situated on approximately 15 acres with expansion capabilities, 2710 South West 18th Street is new construction by Jones Development Company.
"Oklahoma City represents an excellent new market for Monmouth," said Michael P. Landy, COO of Monmouth Real Estate Investment Corporation. "Their economy is one of the strongest in the nation with low unemployment and substantial new investments being made in the region."
The industrial building was built-to-suit for FedEx Ground. It is fully automated and expandable by 34,000 sq. ft. The property is located between the region's airport and downtown area.


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By Tova Cohen
TEL AVIV (Reuters) - Rounds, a free video-chat service that is also a social network, expects to add tens of millions of users with the launch of its mobile phone application.
Israel-based Rounds operates on top of existing platforms with the goal of bringing friends together irrespective of their preferred operating system or device.
Besides video-chat there are photo-sharing features and the ability to watch YouTube videos, play games or listen to music with those contacts, who see exactly what the other sees.
"We are the first hangout network, offering online entertainment with video communication to bring people closer together," Rounds co-founder and Chief Executive Danny Fishel told Reuters.
Rounds has over 6 million users who spend an average of 40 minutes a day "hanging out", mainly via the Facebook app.
Last month Rounds launched its first application for Google Android-based phones and Apple iPhones. It expects mobile to bring the number of users to tens of millions, reaching more than 80,000 new users in the first 48 hours.
"From our perspective, I can be on an Android device and you on Facebook, we merge the contact lists together," Fishel said.
Rounds also gives these "hangouts" an afterlife, allowing users to take pictures of themselves and fuse the images so it appears as if they are physically together. Users can follow, share, like and comment on the photos.
For now Rounds is concentrating on expanding to different networks and will work on bringing in revenue in the second half of 2013. It is considering various models such as micropayments to unlock special video effects.
Competitors include Google Hangouts and Airtime, founded by Napster founders Sean Parker and Shawn Fanning.
Airtime offers video-chatting but not games while Hangouts is only for Google+ and Google Talk users.
"Our platform agnosticism sets us apart," Fishel said. "You can be on Google+, Facebook etc."
Fishel noted that U.S. Marines stationed abroad often use Microsoft's Skype to talk with their families.
"But when they wanted to communicate with a 5-year-old son, after saying how much you've grown, the son had little patience to speak. In Rounds, they have 25-30 minute talks because they can watch YouTube clips and play games together," Fishel said.
Rounds has raised $5.5 million from investors including Verizon Ventures and investment firm Rhodium.
"We believe towards the middle of this year we will do another financing round," he said, adding the company will likely seek to raise $8-$10 million and will look for new and strategic investors.
One purpose of this funding will be to relocate the CEO to the United States and open a marketing office while maintaining research and development in Israel.
(Reporting by Tova Cohen; Editing by Steven Scheer)


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ZAB Approves San Pablo Condos By RICHARD BRENNEMAN
With only two dissents, Zoning Adjustments Board members Thursday approved construction of a five-story condominium project at 2700 San Pablo Avenue.
The building is the latest in a series of condo projects recently announced for the city. Most controversial is the Seagate building, a nine-story project recently approved for Center Street, a half-block west of the Wells Fargo building on Shattuck Avenue.
San Francisco developer Charmaine Curtis and architect David Baker presented plans for their five-story building at the corner of San Pablo and Carleton Street.
Curtis bought the site from developer Patrick Kennedy, whose earlier project for the site had foundered on neighborhood opposition.
While Kennedy's proposal had called for a four-story building, Curtis offered a structure that city staff classified as five floors because ground floor live/work units have an upper level, though the heights of both incarnations are identical.
When she bought the site from Kennedy, Curtis also inherited the use permits previously awarded, paving the way for a potentially speedy approval.
In the eyes of city staff, the new plans constituted a modification of the earlier versions, though the number of units had been reduced and the project had been changed from an apartments plus commercial to condos and commercial.
The Kennedy version had sparked strong neighborhood opposition as well as a lawsuit, prompting the controversial developer to abandon the project and put the land—and permits—up for sale.
"I knew the previous project had engendered a certain amount of controversy," Curtis told ZAB members.
Because of the project's troubled history, she sent notices to 18 neighbors in September and invited them to a meeting where the most common complaint she heard concerned the project's height, a point on which she wouldn't yield.
"I couldn't eliminate one floor and still make money," Curtis said.
With the ZAB's approval, Curtis said, "I'm ready to complete the design and pull permits. I can begin construction next summer and have it complete the following year."
While two neighbors praised the project as a source of neighborhood revitalization, six others spoke against it—including Julie Dickinson, one of the litigants who sued over the previous project.
In addition to height, critics worried about the potential environmental, parking and traffic impacts of construction.
Leslie Marks, who lives immediately behind the site, said she was worried about loss of privacy and garage noise. She also expressed concern about possible exposure to noxious odors and toxins when crews tear down a former gas station of the site.
Tank leaks had polluted the soil around the station, and during the preparation work conducted during Kennedy's ownership, contaminated soils were left uncovered on the site, which Marks said caused her health problems.
Curtis's use permits bar a repeat of the past incident, mandating that toxic-laced soils to be moved offsite immediately upon excavation.
Another concern voiced by both neighbors and ZAB members centered on the four ground floor units, reduced from five in an earlier version of her plans.
One of the original five she had planned was reserved for purely residential use and assigned for sale as an inclusionary unit to be sold at a reduced price.
The Design Review Committee rejected the residential unit, said Senior Planner Greg Powell, so the space was consigned to four live/work units.
Curtis said she simply couldn't afford to sell one of those four as an inclusionary unit because construction costs were too high.
A larger ground floor space at the corner of San Pablo and Carleton is reserved for a commercial tenant, most likely but not necessarily a restaurant, Curtis said.
ZAB member Carrie Sprague asked Curtis why the remainder of the floor was reserved for live/work and not commercial.
Asked why she didn't devote the entire ground floor to businesses, Curtis said, "I would never have tried to develop 5,000 to 6,000 square feet of commercial space. It would just be dead commercial space."
Commissioner Sprague then asked if she could simply remove the upper level loft in the ground floor units to reduce the overall height of the building. "It just seems really high," she said to the applause of the neighbors seated in the back of the room.
Curtis disagreed. "Live/work space are considered high volume spaces," she said.
Member Bob Allen said he understood the concern over height, "but this project meets every planning criterion on the books. What I like about the building is that instead of crowding in every unit they could, they have fewer units, which means fewer cars in the neighborhood. . .It's going to be a beautifully executed building."
Allen moved approval of the project and Jesse Anthony seconded.
Deborah Matthews, in her final session as a ZAB member, said that as a longtime resident of Carelton Street, she welcomed the project, in part because it would discourage the prostitution which has long blighted the area.
"Going from rental to ownership units is also a plus," she said.
David Blake said he was troubled at the loss of the first floor residential unit and by the fact that the live/work units might present a curtained or papered-over face to pedestrians along San Pablo.
He said he was also troubled by the way the city calculated the density bonus granted to the project, which will have inclusionary units on each of the other floors.
The San Pablo project will be the last in the city in which inclusionary bonus space is calculated under the old rules. A new state law coming into effect Jan. 1 will mandate a new basis for calculation, and Sanderson said city staff is studying the changes to bring themselves up to speed.
Then the board voted, and barring an appeal to the City Council, Berkeley has its newest condominium project.
One of those voting for the project was the newest ZAB member, Richard Judd, a lawyer with a degree from Harvard Law School who works for the Oakland real estate law firm of Goldfarb & Lipman, which specializes in affordable housing, redevelopment and municipal law.
Judd was appointed by newly elected City Councilmember and former ZAB member Laurie Capitelli to fill the seat he had just vacated.
The board delayed a decision on developer Richard Schwarzmann's plan to build a five-unit green residential complex at 1414 Harmon Street after neighbors voiced opposition to the loss of daylight the new project would cause. Members indicated they'd be glad to approve the proposal if he submitted a new design with a lower roofline for one of the buildings.
Members also approved plans for renovation of the industrial building at 950 Gilman St., including a reallocation of a quarter of the structure from industrial to office use.
The board took no action on realtor/developer John Gordon's plans to convert seven vacant dwelling units at 1952-1966 University Ave. into office space and construct 3,545 square feet of additional space for two restaurants at the site.
Both the Harmon Street project and Gordon's proposal were continued to ZAB's Jan. 13 meeting.


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Jerry Lewis now claims that his film directorial debut came about when his home studio, Paramount, needed a summer-release Lewis vehicle in a hurry. Jerry and his entourage headed to the Fountainbleu hotel in Miami Beach, and 29 days later returned with The Bellboy. As narrator Walter Winchell (and an actor pretending to be a Paramount executive in a pre-credits bit) explain, the film has no plot and no point; it merely exists for the audience's enjoyment. Lewis plays nebbishy bellhop Stanley, a nonspeaking bumbler who alternates between screwing up and taking his job too seriously. The film's Tati-like gags involve a Volkswagen engine, an overweight guest, a woman with a come-hither voice, a very effective flash bulb, an episode at the Greyhound track, a golf tournament, and a passenger jet. Weaving in and out of the proceedings is Lewis' cowriter (and former drummer) Bill Richmond, made up as the spitting image of Stan Laurel (the real Laurel was approached to play himself, but he gently turned Jerry down, insisting that his aged appearance would disappoint his fans). Miami habitues B.S. Pully, Joe E. Ross, Cary Middlecoff, The Novelites make cameo appearances, as does Milton Berle. Made for peanuts, The Bellboy amassed a fortune, assuring that Jerry Lewis would be permitted to direct many of his own films in the future.~ Hal Erickson, All Movie Guide
Director: Jerry Lewis
Writer: Jerry Lewis
Cast: Jerry Lewis, Alex Gerry, Bob Clayton, Sonnie Sands and Eddie Shaeffer
Sorry there are no upcoming showtimes for The Bellboy


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Mobile has truly unlocked a world of potential when it comes to learning. Organizations now have employees' attention during their downtime and, in some industries, during their off time. Today's mobile-driven environment means that people are always connected to one another, and if we can embrace a few simple design principles, we can also increase their connection with their respective workplaces.
Home » Featured Articles » Six Design Principles for Delivering Mobile Learning that Your Employees Will Flock To
E-Learning and Adaptive Learning Can Improve Cybersecurity Awareness
Cybersecurity is one of those areas of knowledge that requires constant vigilance and constant communication.
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MONUMENT, Colo. (AP) — The fatal shooting of Colorado's top prisons official when he answered the front door at his house highlights a troubling reality for the nation's judges, prosecutors and other legal officials: At a time when attacks on them are rising, it's difficult for them to remain secure, even when they are off duty.
Investigators do not yet know why Tom Clements, 58, was shot around 8:30 p.m. Tuesday at his home just north of Colorado Springs. They could not rule out any possibilities, including that it was a random shooting or that it was an attack related to Clements' job, authorities said.
While small in numbers, similar attacks on officials have been increasing in the U.S. in recent years, said Glenn McGovern, an investigator with the Santa Clara County District Attorney's office in California who tracks such incidents worldwide. He said there have been roughly as many in the past three years — at least 35 — as the entire prior decade. Revenge is usually the motive, he added.
"It's often taking place away from the office, which makes sense, because everyone's hardening up their facilities," he said, adding that he advises prosecutors to constantly assess the safety of their residences.
On Jan. 31, Texas prosecutor Mark Hasse was gunned down as he left his car in the parking lot to the county courthouse. McGovern also counts the rampage by an ex-Los Angeles police officer who killed the daughter of a retired city police officer as part of a plot to avenge his firing.
In Colorado, a prosecutor was fatally shot in 2008 as he returned to his Denver home. In 2001, federal prosecutor Thomas Wales was fatally shot by a rifleman while he worked on a computer at night in his Seattle residence. Both cases remain unsolved.
Attacks on legal officials are still extremely rare, said Scott Burns of the National District Attorneys Association, which counts 11 prosecutors as having been slain in the last 50 years. But he acknowledged that legal officials are vulnerable outside of protected offices and courthouses.
"If someone wants to truly harm or kill them, it's very difficult, frankly. There's not a lot we can do," he said.
Mike McLelland, the district attorney in rural Kaufman county east of Dallas, is a 23-year military veteran. Since his prosecutor, Hasse, was killed on his way into the office, McLelland has warned his staff to be vigilant about their surroundings and possible danger.
"The people in my line of work are going to have to get a lot better at it, because they're going to need it more in the future," McLelland said, adding that he carries a gun everywhere he goes.
Colorado Corrections spokeswoman Adrienne Jacobson would not comment on whether Clements had security at his home. Security was stepped up for other state officials, including Gov. John Hickenlooper, who was ashen-faced as he addressed reporters at the capitol Wednesday.
"Tom Clements dedicated his life to being a public servant, to making our state a better place and he is going to be deeply, deeply missed," Hickenlooper said. In response to a question, he said he believed the rest of his cabinet was safe.
Clements came to Colorado in 2011 after working three decades in the Missouri prison system. He began a review of Colorado's solitary confinement system. He reduced the number of prisoners being held in solitary and closed a new prison built specifically to hold such prisoners — Colorado State Penitentiary II.
He lived in a wooded neighborhood of large, two-story houses on expansive 2-acre lots dotted with evergreen trees in an area known as the Black Forest. Long driveways connect the homes to narrow, winding roads that thread the hills. After word of the shooting spread Tuesday, residents slept with shotguns at the ready, fearful the shooter would return.
It would have been simple to find Clements' house. It took two clicks to get his correct street address through a publicly available Internet locator service Wednesday morning. The listing also included his previous home address in Missouri.
McGovern said he tells his prosecutors to assume that any possible assailants can find their home addresses online and to check for areas they may be especially vulnerable such as neighboring alleys and poorly lit porches.
There is no central database of attacks on legal officials and senior law enforcement executives like Clements.
McGovern has documented 133 of them in the U.S. since 1950 by searching through news accounts and court cases. The total includes 41 killings of judges, prosecutors and other justice and police officials. The assaults usually come with little warning, he said.
Steven K. Swensen, a former U.S. marshal who runs a business consulting on security for court officials, said that attacks on legal staff used to occur in courtrooms. As security has been expanded to protect those rooms, then courthouses, the attacks have spilled out further and further.
"Now we're having more violence off-site, in judge's houses, on their way to and from work," Swensen said.
While Clements generally kept a low profile, his killing comes a week after he denied a request by a Saudi national to serve out the remainder of a Colorado prison sentence in Saudi Arabia.
Clements also recently requested chemicals to execute Nathan Dunlap, who was convicted of killing four people during a 1993 shooting rampage at a Chuck E. Cheese restaurant and is scheduled to become the second person executed in Colorado since the U.S. Supreme Court reinstated the death penalty in 1976.
Clements' father-in-law, Carroll Smith, told The Denver Post that Clements opposed the death penalty.
Earlier this week, Clements spoke to legislators about the need for more security staff in the department's food service areas. Last year, a kitchen worker at a state prison was killed and another was injured in an assault involving an inmate.
Clements is at least the second state prisons chief killed in office. Michael Francke, director of the Oregon corrections department, was stabbed to death outside his office in 1989 in what prosecutors described as a bungled car burglary. A former Oregon prison inmate was found guilty of aggravated murder in 1991 and sentenced to life in prison.
Hickenlooper ordered flags lowered to half-staff at public buildings until the day after Clements' funeral.
Contributing to this report were Associated Press writers Thomas Peipert in Monument, Colo.; Steven K. Paulson, Dan Elliott, Nicholas Riccardi, Alexandra Tilsley and Colleen Slevin in Denver; and Maria Sudekum in Kansas City, Mo.


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Stockton warehouse catches fire
Stockton warehouse catches fire
STOCKTON, Calif. (KCRA) —
A Stockton warehouse that stores walnut products caught fire Friday evening.
Stockton fire crews said neighbors called in the fire at 6040 E. Main St., just before 6 p.m.
Firefighters were able to get the blaze contained by 7 p.m.
The biggest challenge firefighters said they faced was getting water to battle the fire.
Firefighters said they had to use 2,000 feet of hose in order to pump enough water on the warehouse.


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Change in state law aims to help Kern River Valley's cemetery district
LAKE ISABELLA, Calif. (KBAK/KBFX) -- The cemetery district near Lake Isabella has worked for more than a year to get a special bill passed. They say it will bring more support for the small district, and serve the community. On Wednesday, the governor approved the tailor-made law.
"We're such a small community, and it's just not right to see people not able to come here," Kern River Valley Cemetery District general manager Randy Kyte said.
The district runs the seven-acre cemetery off Burlando Road near Kernville. Kyte says they needed an exception to the general state law governing cemetery districts. That's why he worked hard on passage of Senate Bill 159.
"The law states that if you don't have a loved one buried in a cemetery district, then you can't be buried here," Kyte explained. "And, if you don't have property in a district, you can't be buried here." But, SB 159 specifically says the KRV Cemetery District can bury "nonresidents."
"This bill would authorize the Kern River Valley Cemetery District in Kern County to use their cemeteries for up to a total of 400 interments each, not to exceed 40 interments each per calendar years, to inter nonresidents and non-property taxpayers, if specified conditions are met," the bill reads.
Overall, special districts in the state get funds from property taxes, and that's the reason for the general rule on burial of only residents in district cemeteries. But Kyte says there's been no increase in property tax funding in the KRV cemetery district since it was set up in the mid-1950s.
"We haven't had an increase," he said. "We're only getting like $5 a parcel, and so that's really hurt us bad."
But, Kyte thinks the restriction on residents only, is also hurting the entire community. "Sad to say, we've had to turn away a lot of people because the law's the law," he said. He's convinced there are people who grew up in the Kern River Valley, or used to live there, but have since moved away.
And, the cemetery district thinks they are unique enough to require the new exception to the general law.
"It's unique to us because of the seclusion where we're at," Kyte argues. "The closest place for burial is like Bakersfield, which is almost 50 miles away. And there are no other cemeteries around us."
The small district has been grappling with financial hurdles for several years. In 2004, they lost their main water supply when the U.S. Army Corps of Engineers decided it had too much arsenic. The KRV district was forced to switch to well water.
"Now, we have a commercial pump for the well," Kyte said. He adds it costs a lot to run the pump, and sometimes in the summer that can boost power bills to over $1,000 a month.
That's why Kyte has also started a campaign to raise money for a solar power system. "Help improve your next Gated Community" reads a big sign by the cemetery gate. He hopes that gets some attention, and prompts donations for the solar power project. Kyte says the system they want will cost about $58,000. He'd love to raise the money by August.
But, getting the new law is a good step forward. "I want to thank State Senator Jean Fuller," Kyte said. She carried the bill for the district. Kyte himself had gone to Sacramento twice to testify for the measure, and he's relieved it did pass.
"It's a great accomplishment for our community," Kyte told Eyewitness News. "And I think it's going to help a lot of the people that love this valley and support it."


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Sunday September 23, 2018; 02:10 am EDT
Irene and Lee enriched your piles
Have you noticed how quickly your compost pile has shrunk now that the rain has stopped? The umpteen inches of rain between Hurricane Irene and Tropical Storm Lee provided uniform watering to exposed compost piles. This surplus water promoted rapid decomposition by microorganisms and encouraged earthworms to invade the piles.
The microorganisms accounted for the shrinkage. They need at least 50 percent moisture to function properly. Sixty percent is even better. So the deluge gave them optimal working conditions.
The earthworms are another story. We generally do not see earthworms in an active compost pile. That's because of the heat generated by microorganisms. The constant supply of rain cooled the pile, encouraging worms to enter and multiply. Since the microorganisms had already partially digested some of the organic waste, the earthworms had easy access to the residue.
I make it a point to water down my compost pile every week, but I do not add sufficient water to cool the pile because I want the microorganisms to generate enough heat to kill disease-causing organisms, insects and weed seeds. So I normally don't have worms in my compost. Once the piles heat up again, the earthworms will die and we again won't have them.
If you want worms, try vermiculture. Vermiculture is very different from composting. Vermiculture piles are smaller, and the organic waste is kept wet so the piles don't generate heat. In vermiculture, the waste is left undisturbed so the worms reproduce faster. The worms digest the waste, forming worm castings, which — like heat-produced compost — are free of weed seeds, insects and plant-causing disease.
Q You've written in Bay Weekly that Wye Oak clones can be purchased from Maryland Department of Natural Resources. I tried calling the nursery but got no response. Also could not find info online. How can I purchase a seedling?
–Lee Tilton, Tracys Landing
A Call the Ayton State Forest Nursery in Preston at 410-673-7285 or 410-673-2467.
Ask Dr. Gouin your questions at [email protected]. All questions will appear in Bay Weekly. Please include your name and address.


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The most talked about new feature to come out of Android 4.1 Jelly Bean has to be Google Now, Google's answer to the virtual personal assistant craze. Unfortunately, there's only a couple of devices officially capable of running Jelly Bean and using Google Now at the moment, and outside of officially-updated devices, only those who have rooted their devices and have a passion for tinkering have gained unofficial access to the service.
That's all about to change, and it's going to get a lot easier to get Google Now on your rooted Android smartphone. Over on the XDA forums, two flashable .zip files have been made available that bring Google Now to a wide range of Ice Cream Sandwich powered Android devices. One version of the flashable .zip features the entire Google Now experience, offline dictation and all, and one version is a little stripped down, doing away with offline dictation to save on precious /system memory space.
The requirements for running this ported version of Google Now couldn't be simpler. You must have an ARMv7 device, be rooted, be running ClockWorkMod Recovery and have Ice Cream Sandwich.
With all of the ICS ROMs available now, the list of compatible devices is monstrous.
To see if you have an ARMv7 device, you can check this handy list. If you do, and also meet the rest of the requirements, you can get started here.


text: I have the latest update of Norton Anti-virus definitions, which has caught an IRC Trojan. However, I ran a system scan with Norton, which said to quarantine and delete the infected file (which I did), but it still said the computer was infected with the virus. How do I fully remove the IRC Trojan?


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PACIFIC JUNCTION - More than 200 people, including dignitaries from Iowa and Nebraska, turned out for Thursday afternoon's groundbreaking ceremony for the U.S. Highway 34 – Missouri River Bridge.
Noah Murray, a Glenwood resident and Nishna Valley High School graduate, died on Wednesday from injuries he suffered in a swimming accident at Lake Manawa on Aug. 28.
A 21-year-old Glenwood woman died in a single-vehicle accident late Saturday afternoon in Washington County.
Sara Suzanne Sell was a passenger in a pickup truck driven by Micah Harmsen, 26, of Keota.
After two years of discussions, public forums, more than 600 signatures and a unanimous vote of the Green Hills Area Education Association Board of Directors last Monday, the Nishna Valley and Malvern School Districts will put the matter of their proposed reorganization into one district to a public vote on Dec. 7.
Nearly 20 years in the making, the ground-breaking ceremony for the Highway 34 - Missouri River bridge will finally take place Thursday afternoon.
The top ranked Glenwood boys cross country team traveled to Pella on Saturday for the Pella Invitational.
The Rams raced through the competition, which included No. 4 ranked Knoxville and the host No. 6 Little Dutch, for their second straight meet title to open the season.
Johnny Fuller led the Rams to the team championship by running the course in near record time of 16:02. Glenwood finished with 53 team points to easily out-distance runner up Pella's 66 points and Knoxville's 68.
St. Albert scored early and often Friday night as the Falcons rolled to a lopsided 42-7 win over Glenwood in the Rams' 2010 home opener.
TABOR - First and goal at the three yard line. A dive up the middle is thwarted by the Fremont-Mills defense as the first half clock runs out, leaving the Knights trailing 14-8.
The Knights' stop that ended the half wasn't exactly a "play of the game" type but as far as momentum goes, there was no bigger play in the game for Knight head coach Jeremy Christiansen whose team battled back from from a two-touchdown deficit in a 20-8 victory over Adair-Casey on Friday.
Rural Silver City resident Jack May said Monday that Mills County's reluctance to repeal its building permit ordinances is behind his decision to run as Independent candidate by petition in the Nov. 2 election for a seat on the county board of supervisors.
Longtime Mills County resident, Craig Florian of Glenwood recently announced his decision to run for the Office of Mills County Treasurer in the Nov. 2 general election. Florian, an Independent, will be on the ballot as a candidate by petition. He's be opposing Republican Becky Dix Killpack.
Copyright © 2018
The Opinion Tribune is your source for local news, sports, events and information in the city of Glenwood and Mills County, Iowa.


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Rod NicholsPhiladelphia Phillies
Team Official | Ht: | Wt:
|Phillies | More coaches named
Thu, 04 Oct 2012 10:03:14 -0700
The Philadelphia Phillies named roving hitting instructor Steve Henderson as the new hitting coach and also named Triple-A Lehigh Valley pitching coach Rod Nichols as the new bullpen coach. The Phillies will go without a bench coach next year. Mick Billmeyer was promoted to catching coach. Juan Samuel was offered the first base job but has yet to accept.
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|| Source: Philadelphia Inquirer - Matt Gelb


text:
Argentina Orders Google To Censor Suggested Searches
from the it's-algorithmic... dept
Either way, this definitely seems to be a growing global trend of placing the liability and blame for content that people don't like on the intermediaries. Even if we can all agree that the content is offensive and ignorant (and, hopefully, we can), is this really the most sensible response? Personally, I've always found that attempts to censor such content only empower those who already believe in it, because they feel like they're revealing such an important "secret," since others feel the need to shut it down. I tend to think that the best way to respond to bad or offensive speech is with more speech, in order to educate the ignorant.
Either way, putting the responsibility on Google seems silly. Google is just indexing the content. It's not responsible for it. When we seek to put censorship ability on the intermediaries, it feels like we're not dealing with the actual issues. Rather than responding to ignorant speech with more speech, it's trying (and almost certainly failing) to sweep the ignorant and offensive speech under the rug. I greatly prefer a world in which we deal with realities (such as the fact that there are ignorant people out there), rather than hiding them and pretending such people don't exist.


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The Florida Fish and Wildlife Conservation Commission will meet Feb. 13-14 in Orlando to discuss wildlife and fisheries issues, including two new wildlife management areas and more flexible private land deer hunting permits.
One draft rule to be considered Feb. 14 concerns establishing a voluntary private-lands deer management permit program to provide more deer management flexibility. The proposed program is designed to promote wildlife and habitat conservation on private lands in Florida.
The meeting, both days, will be at the "Ports of Call" meeting facility at SeaWorld, 7007 SeaWorld Drive in Orlando, Sessions begin at 8:30 a.m. both days and are open to the public. Anyone wishing to address specific issues or items not on the agenda will be allowed to speak according to guidelines established by the Commission.
On the first day of the meeting, after review of consent agenda items, Executive Director Nick Wiley will present the director's report. After that, FWC Commissioners will take up marine fisheries issues.
On the second day of the meeting, Commissioners will discuss proposed changes for hunting and freshwater fishing, as well as expanding hunting opportunities and public access on nearly 6 million acres of the FWC's wildlife management area system. Most noteworthy would be the addition of two new WMAs in its Southwest Region for the 2013-14 hunting season.
The Commission will vote on proposed amendments that would allow a quota permit holder to take a different guest each day of a quota hunt. Also, hunters would receive reinstatement of their preference points only if they electronically returned their unused quota permit 10 days or more prior to the first day of their quota hunt. Such returned permits would be reissued to other hunters on a weekly basis, instead of once a month.
At the end of the meetings on both days, the public may comment on items not on the agenda. The complete agenda – with links to background reports, details on consent agenda items and information about the meeting – is available at MyFWC.com/Commission.
Anyone requiring special accommodations to participate in this meeting should notify the FWC at least five calendar days before the meeting by calling 850-488-6411. Contact the agency using the Florida Relay Service at 800-955-8771 (TDD) or 800-955-8770 (voice) if you are hearing- or speech-impaired.
The next regular Commission meeting is April 17-18 near Tallahassee, commencing at 8:30 a.m. each day.


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The Girl with the Dragon Tattoo trilogy
Stieg Larsson Trilogy: The Girl With the Dragon Tattoo (Vintage, 2009), The Girl Who Played With Fire (Vintage, 2010), and The Girl Who Kicked the Hornet's Nest (Knopf, 2010)
I've always marveled that some children reared in dysfunctional families grow up to be marvelous, well-adjusted human beings. Others born into loving homes choose an opposite path.
Those who have scratched their way to maturity--even happiness--against the odds now have a new model and patron saint in Lisbeth Salander, the female protagonist of Stieg Larsson's best-selling Swedish trilogy: The Girl With the Dragon Tattoo, The Girl Who Played With Fire, and The Girl Who Kicked the Hornet's Nest.
Purists will argue that a literary character cannot qualify as a saint. There was a time when I, too, delimited my spiritual world along the boundary line separating fact and fiction. A crack appeared in my dualistic standard in 1969, when the Catholic Church admitted that shaky evidence existed to support the historicity of some saints who had long enjoyed annual feast days. Among those was everyone's favorite co-pilot and dashboard bobble head, St. Christopher.
Humans, religious or not, have always drawn inspiration from both legend and historical people and events. So, why not adopt Lisbeth Salander as a saint for our time?
I won't give away the details of her life story here for the sake of those who haven't read the books. Personal discovery of her inner life, values, and unique, but finely tuned, morality is one of the trilogy's great rewards. But I give nothing away by saying that the universe dealt Salander one of the worst hands of any child, fictional or real.
Canonizing Salander challenges us to adjust our understanding about morality. By rigid standards, behaviors that enable her to survive as a functioning human being are immoral. But behavior alone does not determine morality.
For me, the most sensible and hallowed definition of morality is enshrined in my own Catholic tradition. Individual conscience is the final arbiter, superseding everything else. The essence of morality is being human in the best sense, according to each person's capability. Since we are made in God's image, whatever helps us to grow emotionally and spiritually--and thus become more like God--is moral. A decision or action is immoral that causes us to be less than the person God created us to be.
Co-protagonist Mikael Blomkvist says of Lisbeth, a murder suspect in The Girl Who Played with Fire, that she possesses a highly developed sense of morality.
By this he means that her moral compass is trustworthy, and she operates from this core principle. By what right, then, does anyone judge her choices, especially in light of the abuse she suffered as a child and teen from the very adults responsible for guiding and protecting her?
That she arrives at womanhood as a still-moral and functioning human being is miracle enough to merit this fictional character the title of patron for the 21st century.


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Featured News - Current News - Archived News - News Categories
Don't miss the third annual Lewiston Region Tour of Kitchens and Homes, taking place the weekend of June 11 and 12, from 10 a.m. to 4 p.m. at six selected residences in the Lewiston and Youngstown areas.
Event organizers report the Tour of Kitchens is a self-guided tour through six homes. The event is about outstanding kitchens, which are sure to inspire those looking to renovate, rejuvenate or simply dream about fabulous entertaining spaces.
The tour is also about delicious food. Each home will feature a chef from a local restaurant who will offer samples of their signature dish(es).
Guests will be able partake in culinary delights from the following WNY restaurants:
•The Bistro at the Old Fort Inn; Carmelo's Restaurant; The Delaware; DiCamillo Bakery; Macri's Italian Grille; The Nines Catering Company; The Red Coach Inn; Village Bake Shoppe; Wegmans, Niagara Falls; Wine on Third and the Youngstown Village Diner.
The homes will also be paired with a retailer or tablescape artist who will prepare multiple tablescapes - an "artful way to set a table for guests," say organizers. Those on the tour will be sure to get dozens of creative ideas for their next dinner party.
Featured tablescape artists include:
•AJC Interiors; All Season Rental; Canterbury Place; The Country Doctor; Homeward Bound; Summertime Blooms; Tasia Fitzpatrick and vanderpool: design.
The exact locations of the homes and all the details for a fabulous day can only be found in the souvenir program books, which guests will receive with the purchase of tickets, organizers note.
Also included in the tour will be a dessert tasting and wine sampling reception at Water Street Landing, 115 S. Water St., Lewiston.
This will feature desserts from The Village Bake Shoppe and DiCamillo Bakery, as well as a variety of local wines.
Tickets to the tour are priced at $25 per person and are now on sale at the following locations:
•DiCamillo Bakery - Lewiston - 535 Center St.; The Village Bake Shoppe - Lewiston - 417 Center St.; Mode Image - Lewiston - 469 Center St.; DiCamillo Bakery - Williamsville - 5329 Main St.; Homeward Bound - Buffalo - 927 Elmwood Ave.; DiCamillo Bakery - Niagara Falls - 7927 Niagara Falls Blvd.; Kinetic Kitchens - Niagara Falls - 1655 Fashion Outlet Blvd.
A limited number of tickets discounted at $5 off are available at Supermarket Liquors and Wines - Niagara Falls - 8438 Niagara Falls Blvd. (Only 500 tickets available at this price.)
Tickets are also available online at www.TourOfKitchens.net or by phone at 745-7220
A portion of the proceeds from this event will go to benefit the Historical Association of Lewiston and the Porter Historical Society.
For more information, visit www.TourofKitchens.net or call 745-7220.


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Man overturns Lexus after rear-ending wife's Lexus
Man overturns Lexus after rear-ending wife's Lexus
MARION COUNTY, Fla. —
A Georgia man slammed his Lexus SUV into the back of his wife's Lexus SUV on Interstate 75 on Tuesday, overturning his vehicle in the median.
Florida Highway Patrol said the driver, Paul Martin, 51, was traveling northbound on I-75 in Marion County during stop and go traffic.
FHP said Martin was approaching stopped traffic when he veered to the right to avoid it and hit his wife's vehicle -- the two were traveling together, troopers said.
Martin's SUV then hit the median guardrail and overturned onto its left side before stopping in the median facing south.
A passenger in the vehicle had non-life-threatening injuries.
Martin was charged with careless driving.


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November 18 2008 - Campaign Finance Cycle 6 Information
Who must file: THIRTY-DAY POST-ELECTION (Cycle 6) REPORTS are required to be filed by all candidates appearing on the ballot and all political committees and lobbyists that have made expenditures to influence the election of such candidates. In 2008, offices appearing on the ballot include the following:
Filing deadline: Cycle 6 Reports are to be complete as of Monday, November 24th, 2008. The deadline for filing Cycle 6 Reports is the close of business on Thursday, December 4th, 2008.
Smart Client: Campaign finance management and filing software called Campaign Finance Smart Client is available free of charge from the Philadelphia Records Department. The software is intended to allow campaign finance administrators to maintain contribution and expenses databases easily during the periods leading to filing deadlines and then file their required campaign finance records quickly and efficiently. The software can be downloaded here.
The Smart Client software contains many features to support campaign finance filing, such as the ability to maintain records from cycle to cycle and print reports for filing with the City Commissioners office. Samples of Smart Client features:
Other supported filing methods: Philadelphia law requires electronic filing of campaign finance records. Philadelphia accepts submission of campaign finance reports using the following methods:
Campaign finance customer service headquarters: For Cycle 6, the temporary filing headquarters will again be available for the convenience of Philadelphia filers. The filing headquarters will be located in the Marriott Residence Inn, One East Penn Square (corner of Market and Juniper, across the street from City Hall). The room will be identified on the hotel's information board, labeled "Philadelphia Campaign Finance Filing Center" and easily-accessible from the lobby.
Filers wishing to enter their records using city computers or needing assistance with any method of electronic filing are urged to come to the filing headquarters during filing week.
The filing headquarters will be open and customer service staff available from Monday, December 1st, through Thursday, December 4th from 9 AM until 6 PM each day. Computer workstations will be available for those filers wishing to input campaign finance records onsite.
For additional information:
For information about Pennsylvania's campaign finance filing legal requirements, please contact the Campaign Finance Division at (717) 787-5280 or via e-mail at email@example.com, or visit their website.
For questions about Philadelphia's campaign finance laws and reporting requirements, please refer to the Philadelphia Board of Ethics website or call (215) 686-9450.
For information about acceptable electronic filing methods and procedures for Philadelphia, please review the Philadelphia Records Department's Campaign Finance website, send email to firstname.lastname@example.com or call the Records Department at (215) 686-2225.


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Each year, ConsumerLab.com surveys its free e-newsletter subscribers about the vitamins and supplements that they use. The results below are based on 10,260 responses collected in November, 2011. Most respondents used multiple supplements. Respondents gave ratings for 1,551 brands and 906 merchants that they used. Ninety brands and 36 merchants each received at least one hundred consumer ratings. Among these popular brands and merchants, those receiving the highest rating on overall consumer satisfaction within their specific market segment are listed below.
Top-rated Supplement Brands on Consumer Satisfaction:
Top-rated Brands on Consumer Satisfaction for Specific Types of Supplements:*
Top-rated Supplement Merchants on Consumer Satisfaction:
The comprehensive, 129-page ConsumerLab.com 2012 Survey of Vitamin and Supplement Users Report analyzes and compares consumers' satisfaction with popular brands of supplements and supplement merchants. The report also examines the use of 31 specific types of supplements and nutritional products. Results are analyzed by respondent age, gender, and level of supplement use. The report is available for purchase. Custom analyses are also available. For more information or to purchase the report, go to http://www.consumerlab.com/reports/CLSurveyBrochure2012.pdf or contact Lisa Sabin, Vice President for Business Development, at email@example.com.
Brands analyzed in report:
Advanced BioSolutions (Dr. Sinatra), Align, American Health, Andrew Lessman (ProCaps), Barlean's, Berkley & Jensen (BJ Wholesale Club), Biotics Research, Biotivia, Bluebonnet, Caltrate, Carlson Labs, Centrum, Citracal, Cosamin, Country Life, Culturelle, CVS, Designs for Health, Doctor's Best, Douglas Laboratories, Dr. Mercola, Eclectic Institute, Enzymatic Therapy, Equate (Wal-Mart), Ester-C, Gaia Herbs, Garden of Life, GNC, Healthy Origins, Herbalife, Integrative Therapeutics, Jamieson (Canada), Jarrow, Juice Plus, Julian Whitaker, KAL, Kirkland (Costco), Kroger, Kyolic (Wakunaga), Life Extension, MegaFoods, Member's Mark (Sam's Club), Metagenics, Mountain Home Nutritionals (Dr. Williams), Natrol, Natural Factors (Canada), Natural Factors (U.S.), Nature Made, Nature's Bounty, Nature's Life, Nature's Plus, Nature's Sunshine, Nature's Way, New Chapter, Nordic Naturals, NOW Foods, Nutricology, Nutrilite, One-A-Day, Optimum Nutrition, Ortho Molecular, Osteo Bi-Flex, Planetary Herbals, Pure Encapsulations, Puritan's Pride, Purity Products, Rainbow Light, RiteAid, Schiff, Shaklee, Solaray, Solgar, Source Naturals, Spectrum, Spring Valley (Wal-Mart), Standard Process, Sundown, Swanson, Thorne Research, Trader Darwin (Trader Joe's), TruNature (Costco), Twinlab, USANA, Vitacost (NSI), Vitamin Shoppe, Vitamin World, Walgreens (Finest Naturals), Webber Naturals (Canada), Weil (Andrew Weil, M.D.), and Whole Foods.
Merchants analyzed in report:
Advanced BioSolutions, Amazon.com, BJ's, BodyBuilding.com, Costco, CVS, Dr. Julian Whitaker, Drugstore.com, Giant Supermarket, GNC, Healthcare Practitioner's Office, iHerb.com, K-Mart, Kroger, Life Extension, Local Co-Op, Local Drugstore, Local Health Food Store, LuckyVitamins.com, Mercola.com, Mountain Home Nutritionals, Nutrilite, Publix, Puritan's Pride, RiteAid, Safeway, Sam's Club, Shaklee, Sprouts Farmer's Market, Swanson, Target, Trader Joe's, USANA, Vitacost.com, Vitamin Cottage, Vitamin Shoppe, Vitamin World, Walgreens, Wal-Mart, and Whole Foods.
ConsumerLab.com also provides Product Reviews of over 900 supplements and nutritional products based on laboratory testing. Access to the Product Reviews is available through online membership. ConsumerLab.com is a leading provider of consumer information and independent evaluations of products that affect health and nutrition. The company is privately held has no ownership from, or interest in, companies that manufacture, distribute, or sell consumer products.
Copyright 2012 ConsumerLab.com. All rights reserved. It is unlawful to copy, excerpt, or distribute this content, nor use it for any promotional or commercial purpose, without express prior written permission from ConsumerLab.com
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text:
The reason for the difference between light and electron microscopic results is likely that the opacity and electron density of the DAB polymer arise in different ways: its polycyclic structure renders it photon absorbent, but its electron-density results from redox reactions with osmium(
Bahr, 1954
). We reasoned that radicals produced during the long peroxidase reaction might oxidize the relevant functional groups in the polymer, leaving it photon-absorbent but inert to osmium. If this were true, reduction of functional groups on the polymer could restore reactivity to osmium (
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). We tested this hypothesis in transfected HEK cells. When cells were treated with the protocol we had used for retina, theprecipitate was clearly visible by light but not electron microscopy. However, when the HEK cells were treated with a mild reducing agent (5 mM sodium hydrosulfite) between the peroxidase reaction and osmication, they were highly electron-dense ( Figure 1f,g ). This was the case using either conventional osmium staining or an enhanced 'double osmium' staining protocol (rOTO), although the latter showed a slight improvement probably due to the change in theredox state of osmium tetroxide ( Figure 1—figure supplement 1c–f ; see Methods). A similar effect was observed in erythrocytes, in which endogenous heme catalyzes the DAB reaction ( DEleventh High Heels Women Sandals Peep Toe Thin Heels Sale Buy Cheapest Extremely Sale Online Sale Fashionable DMWObEP
When the reduction protocol was applied to retinas, we were able to visualize RGCs that had been tagged with APX, APEX2NES (APEX2 fused to a nuclear export signal) or erHRP (
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, Figure 1—figure supplement 2 and ). As expected, APX and APEX2NES labeled the cytoplasm diffusely ( Figure 1j ) while erHRP labeled membrane-bound intracellular compartments ( Free Shipping Comfortable Hot selling custom made link decorative flowers sheath dress pattern Prices mxAZzph
). Thus they could be used as orthogonal labels, although we have not yet pursued this application. The strength of the signal allowed us to identify small stained dendritic profiles ( Figure 1k,o ) and to view the terminals of RGCs in the superior colliculus, approximately 1 cm from the somata ( Figure 1l ). Remarkably, the reduction protocol actually improved the visualization of the ultrastructure irrespective of peroxidase expression or DAB treatment ( Ladies flat leather women 2017 flat loafer casual shoes Cheap Sale 100% Authentic Free Shipping Sale Online Cheap Good Selling 9b5opG
). Part of the improvement resulted from an increase in the reactivity of membranes to osmium, thereby enhancing membrane-cytoplasm contrast ( Figure 1—figure supplement 4 ). This improvement is also visible in the staining strength of synaptic densities ( Figure 1—figure supplement4a,b ). When we asked blind-to-condition observers to judge the quality of synapses between both conditions, the reduced tissue was selected ~3 times more frequently than unreduced tissue ( Figure 1—figure supplement 4e ). Thus, rather than sacrificing ultrastructure for reactivity or vice versa, this protocol improved both.
Inspect (Inspect.exe) is a Windows-based tool that enables you select any UI element and view the element's accessibility data. You can view Microsoft UI Automation properties and control patterns, as well as Microsoft Active Accessibility properties. Inspect also enables you to test the navigational structure of the automation elements in the UI Automation tree, and the accessible objects in the Microsoft Active Accessibility hierarchy.Inspect
Inspect is installed with the Windows Software Development Kit (SDK). (It is also available in previous versions of Windows SDK.) It is located in the \bin\< version >\< platform > folder of the SDK installation path (Inspect.exe).
can be used to examine accessibility data on systems that don't have UI Automation, but can only examine the Microsoft Active Accessibility properties. To examine UI Automation, UI Automation must be present on the system. For more information, see the "Requirements" section of
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Inspect is installed as part of the overall set of tools in the Windows SDK, it is not distributed as a separate download. The Windows SDK includes all of the accessibility-related tools documented in this section. Get the Windows SDK. (There's also an SDK download archive linked from that page, if you need a previous version.)
To run Inspect , find Inspect.exe in the \bin\< version >\< platform > folder and run it (you don't typically have to run as administrator).
The Inspect window has several main parts:
The commands available in the menu bar are also available in the toolbar. The following image shows the Inspect tool querying the UI Automation properties of the Edit menu element in Notepad.
When you start Inspect , the Tree view shows the location of the currently selected UI element in the element hierarchy, and the Data view shows the property information for the selected UI element. You can navigate the UI to view accessibility information about every element in the UI. By default, Inspect tracks the keyboard or mouse focus. As focus changes, the Data view updates with the property information of the element with focus.
To navigate among UI elements, you can use any of the following:Navigation
The last three options enable you to navigate the tree hierarchy of the UI. The structure of this tree may differ slightly between UI Automation and Microsoft Active Accessibility modes.
The Data view shows the property information of the UI element that is currently selected. You can configure Inspect to show you information about all accessibility properties or a subset of those properties. You can also specify other viewing options, such as whether Inspect should remain on top of other user interfaces, or whether Inspect should highlight a bounding rectangle around the selected element. Once you have configured Inspect to work the way you want, you can begin navigating among UI elements and viewing property information. Inspect saves your configuration settings when it closes and uses them to initialize your next Inspect session.
ttna ttnb Cronos
With regard to the primary area of contention, we recognize that we have done an inadequate job drawing a path between our observations in the zebrafish model and human cardiac disease. In the revised manuscript we have sought to make a clearer, more compelling case with the following changes:
A) Comparison of zebrafish and human titin:
The following text is included verbatim in the revised manuscript and we have included a new Figure (
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) to graphically illustrate the domain organization of human TTN and zebrafish and , as suggested. Our primary objectives in this section are to 1) highlight the similarities between the human and zebrafish Titin proteins, and 2) emphasize that the paralogous ttna and ttnb proteins are both essential for skeletal muscle sarcomere development, creating a scenario where, at least in skeletal muscle, studying a homozygote knockout of one of these genes bears resemblance to studying a heterozygote in mammals.
We have also rephrased the text accordingly. Please see: "As a result of an ancestral genome duplication event, zebrafish include two titin genes: and […] severe disruption of skeletal muscle sarcomeric architecture, highlighting the mutual contribution of the two genes (14)."
B) Tissue-specific quantitative and developmental survey of expression:
In the revised manuscript, we have added 3 new figure panels that characterize expression in zebrafish and mouse by whole mount in situ hybridization (with one probe specific to ) and by quantitative real-time PCR (
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). Our primary observation is that expression is at high levels in zebrafish skeletal muscle during development (∼2:1 ratio of to full-length ), but drops off dramatically during adulthood (∼1:70 ratio). Although the expression in zebrafish heart also appears to be developmentally regulated, it is much lower than in skeletal muscle, with a ∼1:5 ratio at 72 hr and 1:30 ratio in adulthood.
In contrast, the relative levels of in mouse heart are much higher than in skeletal muscle, with nearly a 1:1 ratio of to full-length Titin in E12.5 heart. Thereafter mouse cardiac expression of decreases to ∼20–30% of full-length Titin. Skeletal muscle expression varies widely across development and in different muscle beds with a 1:5 ratio in mouse hindlimb at P2, and between 1:16 and ∼1:1000 ratios in adult extensor digitorum longus (fast-twitch) and soleus (slow-twitch) muscle, respectively.
We indicate that the poor expression of in zebrafish heart can be a possible explanation for the indistinguishable cardiac phenotypes of N- and C-terminal truncation mutants, though the fact that plays a minimal role in cardiac development is probably also contributing to the severity of the mutants.
In addition to the additional Figure 6 , we have included two paragraphs summarizing the tissue-specific and developmental stage results: "We next surveyed the expression levels of in cardiac and skeletal muscle […] expression varies across development and in different muscle beds with a 1:5 ratio in mouse hindlimb at P2, and between 1:16 and ∼1:1000 ratios in adult extensor digitorum longus (fast-twitch) and soleus (slow-twitch) muscle, respectively."
C) Interpretation of lack of variation in truncation mutation severity in heart:
We attribute the stark differences between skeletal muscle phenotypes in N- and C-terminal truncation mutants to 1) the prominent expression of in developing skeletal muscle and 2) the presence of an important functional paralog, , which can substitute for function. Unfortunately, in cardiac muscle the contribution of both of these factors is severely diminished, including low expression of , and a more minimal role for in cardiac sarcomerogenesis.
We are reassured that there is prominent expression of in developing mouse heart, which is in keeping with playing an important role in mammalian heart development and therefore consistent with the lack of intensifying DCM phenotypic severity. Interestingly, others have described a prominent band on protein gels of developing mouse and rat heart tissue at the expected molecular weight of , which is substantially diminished in adulthood. This band has been dismissed as a degradation product – but we speculate that in some cases this is very likely to be .
We touch on the limited cardiac expression of in zebrafish as well as the limited contribution of in heart development as explanation for our zebrafish cardiac results. We also include an additional paragraph in the Discussion: "Our work also proposes an alternative explanation for the consistently observed 'T2' band on protein gels, which migrates at the expected molecular weight of . This has invariably been labeled a C-terminal degradation product, as it reacts with A-band antibodies (25). However, in keeping with expression patterns in development, the T2 band was prominently seen in developing mouse (25) and rat hearts (26), but was markedly reduced in adulthood. It is possible that, at least in some situations, T2 and represent the same isoform."
D) Perturbation induced discrimination of N- and C-terminal heterozygotes:
We agree that perturbation experiments in heterozygotes (such as was done with mice, Gramlich et al, 2009) would have been more consistent with the human disease scenario, although the presence of paralogs complicates this situation in zebrafish. Although we tried isoproterenol (and angiotensin II) at several doses and at several developmental time points and used natriuretic peptide levels as readout, we were not able to consistently distinguish WT fish from heterozygotes (N- or C). Given the low cardiac expression of , we suspect that even with an optimized assay, the distinction of N- and C-terminal truncation mutants from one another would be very challenging. Cronos
This is an excellent point. Our prior approach to NMD estimate was inadequate and involved normalizing total to levels in homozygote null mutants. In the revised manuscript we have performed targeted RNA-Seq of the mutant exons in heterozygote embryos to estimate NMD by looking at allelic imbalance ( Figure 2D ). Comparing raw counts of mutant and WT alleles, we see ∼80% of the mutant transcript degraded, in keeping with established NMD efficacy. Interestingly, we see very little variation across the length of the transcript. We have also performed qPCR to estimate the ratio of to full-length in N-terminal and C-terminal mutants and find the expected result ( Figure 5B ): a high ratio of to full-length for all N-terminal mutants and a much lower rate, more comparable to WT, for the C-terminal mutants. We suspect that some variation in promoter activity for the full-length and transcripts could account for some of the variation in these ratios within the N- and C-terminal mutant groups.
We have modified the text and figures to include this data, which we believe strengthens our story. For example, we now state: "Given that NMD substantially reduces the levels of transcripts with premature termination codons ( Figure 2D ), we reasoned that we should be able to distinguish N- and C-terminal truncation mutants by the ratio of to full-length transcript. In mutants, which would degrade full-length but not , we would anticipate a higher :full-length ratio than in mutants mutants, which should degrade both transcripts approximately equally. As expected, we see a much higher ratio of :full-length transcript levels in mutants than in mutants ( Figure 5B )."
We have now performed qPCR to demonstrate ∼78% knockdown efficiency using our morpholino. Either exon skipping or intron retention arising from morpholino injection should result in an N-terminal truncation on top of the C-terminal mutation. In conjunction with NMD, this intervention should result in residual C-terminal truncated protein being at ∼5% of the level of . Given that we see no amelioration in skeletal muscle function (fish remain immobile) or architecture, it is difficult to reconcile this observation with a dominant negative mechanism. Similarly, for dosage to be responsible for the difference between N- and C-terminal truncations, we would not expect to have seen similar levels of NMD for N- and C-terminal truncations nor see persistence of skeletal muscle pathology with such a low level of mutant C-terminal protein present.
In the manuscript, we have made the following changes: "Knockdown by morpholino was effective (∼78% knockdown efficiency, data not shown) and the combined action of NMD and morpholino knockdown would be expected to reduce mutant ttna protein to only ∼5% of the level of ttnb protein. Nonetheless we were unable to rescue skeletal muscle architecture in mutants (
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). We thus concluded that mutants do not act as dominant negatives and that an alternative explanation was needed."
We have updated this gel to include morpholino knockdown controls as well as loading controls. We have found this gel technically challenging to execute, potentially because of the long running time ∼6 required, but we believe the results are now convincing.
We have modified this section and included 2 new figure panels: 1) one that depicts the position of mutations in DCM patients and control populations or from the senior athletes onto a schematic of the TTN meta-transcript 2) a ratio of mutations found in regions C-terminal to the promoter to those in the N-terminus in different populations. We have eliminated the figure panel that emphasizes the location of senior athlete mutations to the terminal exon of the Novex-3 isoform. In fact, when we referred to the Novex-3 isoform we meant only those mutations mapping to the terminal exon, which is unique to this isoform.
The following text is now included in the revised manuscript: "We compared the frequency and distribution of TTN truncation mutations to those found in unselected controls and healthy volunteers from a recent sequencing study (CTLlit) (7) […] As expected, in end-stage DCM we observed a much higher rate of mutants C-terminal to than N-terminal (30:1), followed by unselected DCM (37:11), literature controls (6:11, 2:2, 10:7 for FHS, healthy volunteers and JHS) and senior athletes (0:3)."
We apologize for creating the impression that our work has resulted in newfound certainty in genotype-phenotype correlations. From our experience taking care of many dozens of families with inherited cardiomyopathies, such determinism is never the case. We have moderated language that makes any such suggestion in the revision. We would like to address some of the above comments and concerns:
1) The presence of 'controls' with C-terminal truncations does not eliminate the possibility that these mutations do in fact have cardiac consequences that fall short of overt heart failure, which was the motivation for studying a hyper-normal senior athlete population. Finding C-terminal truncations in the senior athlete population would have cast greater doubt on whether such mutations are sufficient to compromise cardiovascular fitness.
2) We are encouraged by this report of subclinical skeletal manifestations in DCM TTN patients though we cannot find any published studies that have rigorously documented this.
3) We have eliminated mention of the HCM association with TTN.
4) Our work actually does not address if N-terminal truncations – especially in constitutive exons – are deleterious. We analyzed the senior athletes with the hope that we might find N-terminal truncations though small sample size precludes robust conclusions. We do note that in Supplementary Table 12 of Herman et al, the dozen or so families with evidence of segregation in 2 or more members all had C-terminal truncations.
Please see the following passage in the manuscript: "Our results have two clinical implications for titinopathy patients […] although it is unclear whether there is a restricted developmental window where this might be possible."
[Editors' note: further revisions were requested prior to acceptance, as described below.]
eLifeReviewer #2: Reviewer #3: ttnb Cronos ttna ttna
In the revised manuscript we have added two paragraphs (shown below) at the beginning of the Discussion to summarize our findings and highlight the strengths and limitations of the system we have used. Although we firmly believe that our findings represent a substantial advance in this field, with strong biological and clinical implications, we recognize the challenges of studying adult onset autosomal dominant diseases with incomplete penetrance in any sort of animal model. Even if one can bring about phenotypic distinctions with artificial perturbations or genetic modifiers or use increasingly sensitive phenotyping methods to capture differences, one cannot be sure such stresses or distinctions are in fact relevant to the human disease process. Furthermore, TTN brings about an even greater challenge because of the technical challenge of performing rescue experiments with a 110 kilobase transgene.
With regard to Reviewer 3's final comment, we do not understand the statement of how a knockdown of a mutant allele in the setting of a WT alternative () is of no value in assessing a dominant negative mode of action and instead "emphasize(s) the failure of the zebrafish experiments as described to capture the biology of either human skeletal or cardiac titin disease". If one accepts that is rescuing the null, this is analogous to performing allele specific knockdown of a mutant protein in a heterozygote and looking at phenotypic impact. In the manuscript, we stress: "Loss of function experiments across a number of systems have demonstrated Titin's importance in diverse aspects of sarcomere development and function, including the ordered assembly of sarcomeric constituents […] Nonetheless, taking all of our results together, we find it a highly plausible explanation that a superimposed deficiency in expression of the protein product would result in the more severe forms of human cardiac disease seen in DCM patients with C-terminal TTN truncations."SFZB Summer Zapatos Mujer Rough Sandals High Heel Casual Enjoy W68O1
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Article IV also grants to Congress the authority to admit new states into the Union. Since the establishment of the United States in 1776, the number of states has expanded from the original 13 to 50. Each new state has been admitted on an equal footing with the existing states. [20] It also forbids the creation of new states from parts of existing states without the consent of both the affected states and Congress. This caveat was designed to give Eastern states that still had Western land claims (including Georgia, North Carolina, and Virginia), to have a over whether their western counties could become states, [19] and has served this same function since, whenever a proposal to partition an existing state or states in order that a region within might either join another state or to create a new state has come before Congress.
Most of the states admitted to the Union after the original 13 were formed from an
organized territory
established and governed by Congress in accord with its
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under Article IV, Section 3, Clause 2 . [37] The outline for this process was established by the Northwest Ordinance (1787), which predates the ratification of the Constitution. In some cases, an entire territory has become a state; in others some part of a territory has.
When the people of a territory make their desire for statehood known to the federal government, Congress may pass an enabling act authorizing the people of that territory to organize a constitutional convention to write a state constitution as a step towards admission to the Union. Each act details the mechanism by which the territory will be admitted as a state following ratification of their constitution and election of state officers. Although the use of an enabling act is a traditional historic practice, a number of territories have drafted constitutions for submission to Congress absent an enabling act and were subsequently admitted. Upon acceptance of that constitution, and upon meeting any additional Congressional stipulations, Congress has always admitted that territory as a state.


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Reports of area college football games involving Mount Union, Malone and Walsh.
Burke keys Raiders' attack
MOUNT UNION 30, BALDWIN WALLACE 7 Mount Union quarterback Kevin Burke threw for two touchdowns and ran for another as the Purple Raiders won an Ohio Athletic Conference home game over the Yellow Jackets to remain undefeated.
Burke was 15-of-23 passing for 197 yards with an interception. He threw touchdown tosses of 11 yards to Luc Meacham and 57 yards to Jack Nicholls. Burke also ran the ball seven times for 34 yards, half of that coming on the game's first score in the opening quarter.
Nicholls hauled in seven passes for 109 yards — his fourth 100-yard game of the season.
Bradley Mitchell ripped off 114 yards on 18 attempts to ignite the Mount Union ground game. It was Mitchell's second straight 100-yard effort and third of the season for the freshman back from Michigan,
Mason Minnich added 68 yards and a touchdown for the Purple Raiders, who ran for 238 yards.
Baldwin Wallace quarterback Kyle Ohradanzky (McKinley) ran for a touchhdown and was the leading rusher for the Yellow Jackets with 56 yards on 18 carries.
Ohradanzky also was 6-of-9 passing for 84 yards with an interception.
The Mount Union defense recorded seven sacks, with Alex Kocheff and Matt Fechko recording two sacks apiece for the Purple Raiders.
Ford drives Findlay
FINDLAY 35, MALONE 21 Findlay running back Daiquone Ford ran for three touchdowns to help the Oilers to a GLIAC win over Malone at Fawcett Stadium.
Ford piled up 209 yards on 37 carries and scored touchdowns in each of the first three quarters.
Anthony Linnear added 66 yards on 14 carries and also ran for a touchdown for Findlay.
Malone had a difficult time in the passing game in gusty winds. The Pioneers had three players with pass attempts, but were just 9-of-20 for 175 yards with an interception.
The Pioneers had six passes broken up and one blocked at the line of scrimmage. Malone quarterbacks also were sacked three times.
Jordan Phillips caught four passes for 92 yards, including a 20-yard touchdown.
Justin Davis contributed a rushing touchdown for the Pioneers, finishing with 88 yards on 18 carries.
Walsh can't hold on to early lead
GLENVILLE STATE 33, WALSH 21 Rahmann Lee rushed for 216 yards and one touchdown to lead Glenville State to a GLIAC win over Walsh at Morris Stadium.
Glenville State jumped out to an early 6-0 lead in the first quarter, but the Cavaliers' Matt Matuska took the ensuing kickoff and returned it 92 yards to take a 7-6 lead.
Walsh (3-7, 3-5) never led again.
Jason Fracassa completed 19 of 43 passes for 191 yards for the Cavaliers. Krishawn Hogan was the leading receiver for Fracassa with six catches for 72 yards.
Aaron Male, who had 11 carries for 33 yards for Walsh, added a 2-yard touchdown run late in the game.


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North Carolina legislators are eyeing a $9 million cut to the state wildlife agency's budget due to economic struggles, a move that would result in layoffs within the agency.
The $9 million cuts would be for each of the next two years and would be almost half its funding. The N.C. Wildlife Resources Commission handles hunting, hunter education, boating boater education, state game lands and myriad other outdoors-related tasks.
Republican legislators looking to trim the budget didn't overlook the WRC.
"This is the toughest one," said six-term Sen. Andrew Brock, a Republican who represents Davie, Iredell and Rowan counties. Brock said he hoped the money could be restored to the budget after the economy improves.
"When you've got support for hunting and fishing like you do now in the General Assembly, we'll try to get that back as soon as possible," he said in this Raleigh News & Observer report. "But with prisons closing down, and no one getting a great project built anywhere, we looked at each other and said, 'We all suffer in this one.' "
A plan to raid a wildlife endowment fund set up for use in the future was thwarted after stiff opposition.
Read the full report here.


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CHEYENNE, Wyo. (AP) — While Washington DC squirmed this week over the impending federal government spending cuts, Wyoming lawmakers in Cheyenne — some 1,500 miles west — went about business as usual, wrapping up a legislative session that saw them vote to continue salting away hundreds of millions in the state's already bulging savings accounts.
Knocking Washington is almost a sport in Wyoming.
The state's two U.S. senators and lone congresswoman are all Republicans and all served in the state's overwhelmingly Republican Legislature before going to Congress. This year, as they always do, they returned from Congress to Cheyenne and regaled their former comrades with stories about how miserable and inefficient Washington is compared to Wyoming.
Wyoming Gov. Matt Mead, a Republican, had much the same message this week on his return from a trip to Washington where he and other governors met with President Barack Obama. Mead said the prospect of looming federal spending cuts took center stage at all the meetings.
"There was disagreement among the Democrats and the Republicans over whether that was the right amount of cuts or more cuts needed to be made," Mead said of his fellow governors. "But what there was agreement on was that by setting up something that they all say that they don't want now, and doing it on the 11th day, the 11th hour, that's no way to run a business. That's no way to run a railroad."
"I think that's one of the fundamental things that's wrong with Washington, D.C. — that they won't make those tough decisions," Mead said.
Wyoming perennially gets ranked near the top among the nation's best-managed states. The nation's least populous state with just over 560,000 people, it has virtually no debt and roughly $16 billion in various savings accounts, many of which are inviolate under the state Constitution. It's set to finish the current fiscal year with another $1.6 billion in its "rainy day fund."
Wyoming lawmakers, meanwhile, seemingly vie to see who among them can claim to be the most fiscally conservative. They rejected Mead's suggestion in this session to put less money into permanent savings to plump up the rainy day fund against the possibility that the state might need the money in years to come as energy revenues are expected to flat-line. Instead, the lawmakers say they want to continue to build permanent savings so investment proceeds can continue to help fund state government operations.
State financial analysts predict Wyoming's general fund revenues, which rely heavily on taxes on mineral production, will rise from an estimated $1 billion in fiscal year 2013 to only $1.1 billion by fiscal year 2018.
Faced with the mere suggestion of future revenue declines, the Legislature followed through on Mead's suggestion to cut most state agency budgets 6.5 percent this session. Mead vetoed legislative language that would have required state agencies to submit plans for similar budget cuts in the coming two years. He said there's no point in going through the considerable work of drawing up the cuts ahead of more concrete revenue projections.
Well managed as Wyoming may be, good old dumb luck certainly also plays a role in the state's current good fortune. The nation's largest coal producer, Wyoming's also rich in natural gas, oil and other minerals. Taxes on that energy production, although now flattening out following a boom in the last decade, still keep state coffers full despite the state's lack of personal or corporate income taxes.
Still relatively flush with cash despite a recent slowdown in the energy markets, the Wyoming Legislature this session voted not to accept the federal government's offer of $50 million to expand the Medicaid program to serve an additional 17,600 additional low-income adults. The few Democrats in the Legislature fought for the Medicaid expansion, one of the cornerstones of the federal Affordable Care Act.
Despite a state health department study that concluded expanding the low-income health program would save the state over $125 million over seven years, most lawmakers said expansion wouldn't be prudent when there's no guarantee the federal government can follow through on its promise to pay for it in years to come.
Many are still stinging from recent congressional action to strip away more than $700 million in Abandoned Mine Lands funding that it had agreed to pay Wyoming in coming years.
The health department took the position that the state should take the federal money for Medicaid expansion, and that if the money goes way, the state should stop serving the expanded population, Senate Majority Floor Leader Phil Nicholas said.
"There are a lot of us who believe you simply can't do that," the Laramie Republican said. "It's not a faucet that can be turned off and on, and once extended, those hands of aid can never be withdrawn."


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|Tucson, Ariz.
|High School:
|Previous College:
|Political science
2013: Made 36 appearances with 33 starts, including 29 starts at first base and four as the designated hitter … Finished with a .336 batting average (41-for-122) with six doubles, a home run, 17 runs scored and eight RBIs … Recorded a .996 fielding percentage with team-highs in putouts (251) and chances (257) with five assists and just one error … Led Yale in batting average (.336), slugging (.410), on-base percentage (.391), hits (41), total bases (50), stolen bases (7), and was second on the team in runs scored (17), doubles (6), and home runs (1) … Recorded a team-high 12 multi-hit games, reaching base in a team-best 12 straight games and matched a team-best eight-game hitting streak.
2012: Primarily a second baseman, made 42 appearances with 42 starts (33 starts at second base), batting .234 (34 hits in 145 at-bats) with 10 doubles, a triple, two home runs, 18 RBIs and 20 runs scored. … Finish second on the team in doubles (10), home runs (2), RBIs (18), and stolen bases (6), fourth in slugging (.359), total bases (52), runs scored (20) and sacrifice bunts (4), and fifth in batting average (.234). … Defensively, recorded a .957 fielding percentage (101 assists, 78 putouts, 187 chances) with 27 double plays. … Went 2-for-3 with a home run, three RBIs and two runs scored in a 7-4 win over Sacred Heart on April 17. … Belted the game-winning double, going 3-for-5 with two doubles, a triple and two RBIs in a 5-4 win in 12 innings over Holy Cross on April 24. … Belted his second career home run, a three-run homer, in the ninth inning to rally Yale to a 10-8 win at Brown in the second game of a doubleheader, going a stellar 4-for-6 with a home run, two doubles, six RBIs and two runs scored in the victory. … Closed the season strong with a .354 average (23-for-65) over the final 18 games with two home runs, a triple, eight doubles and six of his nine multi-hit games.
2011: Appeared in 35 games with 34 starts, batting .300 (36 hits, 120 at-bats) with a .351 on-base percentage and .375 slugging percentage. … Belted nine doubles with 24 RBIs … Finished third on the team in RBIs (24), fourth in total hits (36), doubles (9), and sac bunts (3), and fifth in total bases (45). … Also made one relief appearance, allowing one hit and no runs.
Before Yale: Four-year letterman at Salpointe Catholic High School in Tucson, Az. … As a junior, was voted the 5A Southern Arizona Player of the Year … Earned 5A First Team All-State and Junior Sunbelt All-Tournament Team honors as a shortstop and pitcher as a junior … As a senior, was again selected to the 5A Southern Arizona First Team while garnering Second Team 5A All-State honors.
At Yale: Resident of Calhoun College. Political Science major.


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In the United Kingdom, Parliament will take up a proposal to give National Health Service patients the right to seek private health care if they have been kept waiting for an appointment with a specialist for more than four months. Cancer patients, in particular, have evidently been removing themselves from the queue the hard way.
But the problem isn't only with specialized forms of treatment. The London Times quotes Jennifer Dixon of the Nuffield Trust:
"It would not only give patients enforceable health care entitlements but it would also prevent managers and clinicians from controlling waiting times as a way of limiting demand and saving money," she said. "In the past requirements to make financial savings often resulted in hospitals stopping routine surgery for a couple of months before the end of the financial year."
What a system! It beggars belief that Barack Obama and Democratic leaders in Congress want to reproduce the fiasco of socialized medicine here in the U.S.


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Kelly Akashi
January 01, 2018, 10:31am
You Had Me At Hello: 150 Contemporary Artworks That Altered My Consciousness - Part 1
I look at a lot of art. Some of it good, some of it bad. Every once in a while, I come across artwork that fundamentally changes me, even if I don't understand it at the time. A friend of mine recently asked me which works had had the greatest impact on me over the years, so I compiled my thoughts. This is not a greatest hits list and many artists I love are not included in it. These are all works that have been, for whatever reason, seared into my brain. To be honest, there are a number of artists on this list whose overall practice I am not a particular fan of, yet, they got to me at least once. – Steven Zevitas, Publisher
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Hi there,
I went to this site e-lactancia.org and it was marked as safe while breastfeeding, so I took one dose earlier this morning.
Later I read about this being a dangerous medication (arrythmias, liver toxicity) and that large amounts went into breastmilk! I'm already panicking and wondering if I should pump and dump until blood levels decrease?
I'm really anxious here because we've been struggling with lacth problems and I absolutely want to avoid nipple confusion (if I have to pump and dump).
Please help!