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use NOXA shRNA control vector, pWZL-shNOXA-blast were obtained by subcloning the p53 and NOXA fragments from pBabe-shp53- puro and pBabe-shNOXA-puro, correspondingly, which were kindly provided by Dr. D. Ginsburg. PLXSN-GSE56-neo was obtained from subcloning GSE56 BamH1fragment from pBabe-GSE56-puro which was kindly provided by Dr.AW Gudkov into pLXSN. For mouse p53 knockdown, the p53 short hairpin RNA vector and its human Rb shRNA control vector were kindly provided by Dr. SW. Lowe. The results are presented as a mean6SD of two duplicate runs from a representative experiment. Western blot analysis For Western blotting, total cell extracts were prepared, 50 mg protein of each sample was fractionated by gel electrophoresis, and transferred to nitrocellulose membranes. The following primary antibodies were used: rabbit polyclonal anti-p53, rabbit polyclonal anti-p21, mouse monoclonal anti a-SMA, mouse monoclonal anti SMMHC, mouse monoclonal anti-GAPDH. Horseradish peroxidase anti-mouse and anti-rabbit were used as secondary antibodies. The signal was detected by the super-signal-enhanced chemiluminescence system. Quantative Real Time PCR Supporting Information p53 inhibits the adipogenic differentiation program in the MBA-15 cell line. Confluent cultures of MBA-15-shp53 and control cells were subjected to induction of adipogenic differentiation by treatment with medium containing 10 mg/ml insulin, 10-6M dexamethasone, 0.5 Mm 24001208 3-Isobutyl-1-methylxanthine or with control medium. The cells were grown for three weeks with medium replacement once in three days. Total RNA was isolated before to the induction of differentiation as well as 7 and 21 days later. Relative expression of PPARc, CEBPa, Ap2 and AdipoQ p53 Regulates Differentiation were determined by QRT-PCR analysis. The results of QRTPCR are presented as a range of two duplicate runs after normalization to HPRT control. Adipogenic differentiation was assessed using Oil Red O staining for lipid droplets. Found at: doi:10.1371/journal.pone.0003707.s001 QRT-PCR are presented as a range of two duplicate runs after normalization to GAPDH control. Found at: doi:10.1371/journal.pone.0003707.s002 Acknowledgments VR is the incumbent of the Norman and Helen Asher Professorial Chair Cancer Research at the Weizmann institute. Testicular germ cell tumors are responsive to cisplatin-based therapy, even when metastatic. However, 15 20% of patients are refractory to treatment or undergo late relapse and cisplatin-based therapies are associated with life-long toxicities. These represent important clinical issues to overcome with improved therapies. Pluripotent embryonal carcinoma cells are proposed to represent TGCT stem cells and to be the malignant counterparts to embryonic stem cells. There is recent evidence to suggest that ES/EC cells are similar to undifferentiated somatic cancers and cancer stem cells but dissimilar to normal adult tissue stem cells. Thus, strategies devised to target EC cells may have therapeutic utility toward somatic cancer stem cells that Trametinib web possess ��ES-like��signatures while sparing normal adult stem cells. Human cancers have global DNA 14871500 hypomethylation including hypomethylation of repetitive elements coupled with hypermethylation of CpG islands at specific tumor suppressor gene promoters. There are three main DNA methyltransferases in mammals, DNMT1, DNMT3A and DNMT3B. DNMT1 is mainly responsible for maintenance DNA methylation while DNMT3A and DNMT3B mediate de novo methylatio
Month: April 2017
Extracts from these cells were incubated with a 34-bp duplex substrate containing a single uracil residue at position 16 in a reaction containing dCTP and chain terminator ddGTP to measure pol b specific incorporation
incorporation of cHS4 sequences into the SB and PB vectors could lead to increased gene expression levels from the transgene cassette. Similarly, insulated SB and PB clones containing 1 to 3 transposon insertions exhibited between 1.4 and 2.3-fold increased expression levels. However, the positive effect of the cHS4 insulator on transgene 5 cHS4 Insulation of Transposon-Delivered Transgenes expression levels was most pronounced in clones with a high copy number, where the increase in mean MFI was observed to be as high as 3.7-fold for SB clones and 2.7-fold for PB clones at day 56 of passage. In Tol2 clones, mean MFI values were consistently lower for clones carrying insulated transposons compared to clones containing uninsulated transposons, suggesting that incorporation of cHS4 sequences into the Tol2 vector did not lead to a protection against repressive position effects. In summary, these results indicate that the cHS4 insulator did not have a long-term stabilizing effect on transgene expression from SB, PB, and Tol2 vectors in ARPE-19 cells, but that inclusion of cHS4 sequences had an overall beneficial effect on the level of transgene expression from integrated SB and PB transposon vectors. 6 cHS4 Insulation of Transposon-Delivered Transgenes Increased transposition of insulated SB transposon vectors in ARPE-19 and HeLa cells In the context of SB vectors, the stable transfection rate of cHS4-insulated vectors is higher than the uninsulated counterpart. The number of resistant colonies obtained in colony formation assays is affected by several factors, including transfection efficiency, transposition activity, and expression levels of the resistance gene. To investigate in further detail, if the increase 22803826 in stable transfection rate observed for the pSBT/ cHS4.RGIP.cHS4 vector was due to better transposition of the insulated element, we performed a quantitative PCR assay in ARPE-19 cells to quantify excision circles formed after transposon mobilization from plasmid DNA. A SB100X- or iPB-expressing plasmid, in which the ampicillin resistance gene had been replaced by a chloramphenicol resistance gene, was transfected into ARPE-19 cells together with insulated or uninsulated transposon plasmid. Transfections of transposon plasmid in the absence of transposase were included as negative controls. Two days after transfection, low-molecular weight DNA was extracted, and real-time qPCR analysis was performed using a primer set R-547 flanking the transposon excision site. To account for variations in template DNA input, a PCR specific for the Amp gene in the transposon plasmid backbone was utilized to normalize the excision circle data to the amount of transposon plasmid recovered after transfection. Whereas no difference in the amount of excision circle products was observed for transfections with PBT/RGIP and PBT/cHS4.RGIP.cHS4, a 1.3-fold increase in excision circle formation was observed for transfections with SBT/cHS4.RGIP.cHS4 compared to transfections with SBT/ RGIP. Although this increase was not statistically significant, we reproducibly observed higher levels of excision from the SB vector harboring the insulators, suggesting that 10884520 the increased stable transfection rate, obtained by flanking insulator sequences in the SB transposon vector, was partly caused by an increased level of transposon mobilization. To validate this finding in another cellular context, we investigated stable transfection rates and plasmid mobilization of SBT/RGIP
The WRN acetylation site enacted by p300 resided within a region of WRN that harbors the HRDC and C-terminal NLS domains
0 V, gels were dried and analyzed by autoradiography. Ligation activity is expressed as percent of ligated substrate. derivative clones 1, 3 and 7 expressing the mitochondrial version of hLIG1, LIG32/2loxPLIG42/2mts-hLIG1. Cells were maintained in the exponential phase of growth by daily dilution in fresh growth medium. Human LIG1 mRNA level measured by realtime PCR in clones 1, 3 and 7 of LIG32/2loxPLIG42/2mts-hLIG1 cells normalized to that measured in clone 3. Results of independent determinations with two primer pairs were used to calculate the indicated means and standard errors. Western blot analysis of LIG1 protein level in clones 1, 3 and 7 of the LIG32/2loxPLIG42/2mts-hLIG1 mutant, of the LIG32/2loxPLIG42/2 DNA Ligases in Alternative NHEJ mutant, and of HeLa cells. A mouse monoclonal antibody recognizing human but not chicken LIG1 was used. GAPDH is used as loading control. LIG3 mRNA level measured by realtime PCR in clones 1, 3 and 7 of the LIG32/2loxPLIG42/2mtshLIG1 mutant and the parental LIG32/2loxPLIG42/2 cells, normalized to the levels measured in wt cells. LIG42/2mts-hLIG1 cells after treatment with 4HT for 5 d to generate their LIG32/2LIG42/2mts-hLIG1 cells. LIG32/2loxP LIG42/2 cells are used as controls and mRNA levels are shown normalized to the wt. Western blot analysis of Lig3 protein level in LIG32/2loxPLIG42/2, LIG32/2Cdc9 cells and clones 1, 3 and 7 of LIG32/2LIG42/2mts-hLIG1 cells obtained after a 5 d incubation with 4HT. GAPDH is a loading control and purified human LIG3b a positive control. Representative gels of in vitro DNA end joining of SalI-linearized pSP65 plasmid using whole cell extracts prepared from clones 1, 3 and 7 of the LIG32/2loxPLIG42/ 2 mts-hLIG1 mutant, before and after treatment with 4HT for 5 days. The linearized input substrate and the products of end joining are indicated. Similar results were 12504917 obtained using 2 mg of whole cell extract. 2loxP Phosphoinositide 3-kinases generate lipid second messengers that regulate a broad variety of cellular responses such as growth, cell cycle progression, differentiation, vesicular traffic and cell migration. PI3K activity is critical in a wide variety of normal and pathological physiological responses, including immune regulation, metabolic control and cancer. However, despite the importance of this signalling system, very little is known about the regulation of PI3K gene expression under normal and disease conditions. The PI3K family is divided into 3 classes. Class I PI3Ks are acutely activated upon receptor stimulation and are heterodimers consisting of a p110 catalytic subunit in complex with a Ridaforolimus regulatory subunit. The class I PI3Ks are further subdivided into class IA and IB, depending on whether the catalytic subunit is in complex with an SH2-domain containing regulatory subunit or with the p101 or p84 regulatory subunits, which lack SH2 domains. Mammals have 3 class IA p110 catalytic subunits, p110a, p110b and p110d, encoded by 3 distinct genes, PIK3CA, PIK3CB and PIK3CD, respectively. These p110 isoforms interact with 18772318 p85, of which there are at least five different species, called p85a, p55a and p50a and p85b and p55c. p110c is the only class IB PI3K catalytic subunit and occurs in complex with p101 or p84, which have no homology to p85. Class I PI3Ks can be activated by tyrosine kinases or GPCRs . Tissue distribution and the regulation of PI3K expression has recently been reviewed. Whereas p110a and p110b appear to have a broad tissue distributi
The miRNA-mediated suppression of elongation and termination of translation are less well characterized, although recent studies indicate that miRNAs can block elongation of translating polysomes by causing them to cease translation and `drop-off'
nswell inserts were placed in a 24-well plate containing EBM2. EPCs were added to the upper chamber of the well, without FBS. Cells were allowed to migrate from the upper to the lower chamber for 12 h at 37uC. Non-migratory cells were removed from the upper chamber by wiping the upper surface with use of an absorbent tip. The number of migrating cells was counted in 5 different high-power fields per insert. Capillary-like tube formation was analyzed by use of Matrigel Matrix. EPCs were placed in 96-well plates pre-coated with solidified Matrigel Matrix and cultured at 37uC for 24 h. Capillary-like tubular structures were photographed, and the number of incorporated EPCs in tubules was determined in 5 random fields. 4 Rehmannia Glutinosa Protected Infarcted Myoccardim 5 Rehmannia Glutinosa Protected Infarcted Myoccardim 6 Rehmannia Glutinosa Protected Infarcted Myoccardim A tube was defined as a straight cellular segment connecting 2 cell masses . ELISA ELISA was used to measure cardiac troponin T and brain natriuretic peptide concentration in serum for left ventricular function evaluation, by use of a BNP kit and Tn-T kit. Briefly, standards and diluted serum of rats were added into the pre-coated 96-well plates and incubated for 30 min in 37uC. After a washing with PBS, the horseradish peroxidase-conjugated anti-body was added for 30 min incubation at 37uC. After a washing by PBS, the tetramethylbenzibine substrate was added. After reaching the desired color density, the reaction was terminated by stop 20171952 solution. OD450 was determined by use of an ELISA plate reader. Each samples repeated in 3 wells. Rehmannia Glutinosa Protected Infarcted Myoccardim 8 Rehmannia Glutinosa Protected Infarcted Myoccardim 9 Rehmannia Glutinosa Protected Infarcted Myoccardim times. The final concentration of RGE used to stimulate EPCs was 25 mg/ml for CXCR4 and 50 mg/ml for SDF-1a. Control, culture with EBM-2 without RGE for 72 h. RGE groups, stimulation for 6, 12, 24, 48 and 72 h. EPCs' tube-formation capacity in vitro. C, control group culture with only EBM-2. AMD, purchase PKC412 pre-stimulation with AMD3100 for 1 h and then RGE at 25 mg/ml for 48 h. RGE25-48 h, RGE at 25 mg/ml for 48 h. RGE50-24 h, RGE at 50 mg/ml for 24 h. Data are ratio of EPCs integrated in tubular-like structure and the tubes involved. Each test was repeated 3 times and every group had 3 repetitive wells on one 6-well plate. Data are mean 6 SD. 'P,0.05, P,0.01 vs. all the other groups. doi:10.1371/journal.pone.0054303.g007 Histology and Immunechemistry Myocardial tissues in the left ventricle of rats were removed and fixed in 4% pre-cooled paraformaldehyde for 72 h, then embedded in paraffin, and sectioned into slices 14530216 5 mm thick. Poley's stain was used to assess the ischemic myocardial area. Images were visualized under an optical microscope at 6200 magnification. Myocardial tissue sections underwent the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling using an in situ detection kit following the manufacturer's instructions. The TUNEL apoptotic index was determined by calculating the ratio of TUNEL-positive cells to total myocardial cells. Immunohistochemical staining involved standard techniques as described. Briefly, endogenous peroxidase activity was inhibited by incubation with 3% H2O2. Sections were blocked with 5% calf serum in PBS and incubated overnight at 4uC with the monoclonal antibodies: anti-VEGFR2, anti-CD133 and antiCXCR4. After a washing with PBS, sections were i
miR-24 Blocks p16 Translation the miR-24 that co-sedimented with polysomal fractions did appear to associate with actively translating mRNAs
according to the protocol supplied. Results FGF4 and RA direct differentiation of PDX1+ cells from Activin A/Wnt3a-treated hESCs The pivotal role of 19276073 RA and FGF4 in endoderm and pancreas development led us to investigate their role in directing differentiation of putative DE, obtained through the frequently used three-day Activin A/Wnt3a induction protocol , into PDX1+ posterior foregut endoderm. So far, FGF4 has not been tested for its activity in patterning ESC-derived gut endoderm. In the absence of RA, FGF4 was unable to induce PDX1 expression. Since it was previously shown that RA promotes differentiation into PDX1+ cells when added four days after the AA-induction, we tested whether FGF4 synergized with RA in directing DE into PDX1+ cells. Indeed, PDX1 expression measured on day twelve increased when FGF4 was added directly after AA-induction and before the RA-treatment. Notably, FGF4 exhibited its effect on PDX1 expression in a concentration-dependent manner. Importantly, endogenous expression of FGF4 is only detected in undifferentiated cells and not at later time-points. To further optimize the protocol, the timing of RA addition was considered. In fact, the timing of RA addition has in most previous efforts been rather arbitrary, based on the fact that it should be added rather soon after the DE-induction. Logically, the timing of RA addition should be based on RARb expression, which so far has not been determined. Therefore, we examined the timing of RARb expression after AA-induction. Interestingly, RARb was upregulated directly after the AA-induction on day four, and 21138246 subsequently downregulated in the absence of any exogenous growth and differentiation factor . Based on these findings we then tested various combinations of FGF4 and RA to achieve optimal induction of PDX1 expression during a twelve-day period. Moreover, PDX1 expression increased at day 12 when RA was added at day four compared to at day eight. Further optimisation of the protocol revealed that the highest PDX1-expression level was obtained when RA was kept throughout the whole protocol, i.e. for 13 days after the activin induction. Subtraction of RA at earlier time points diminished the relative expression of PDX1. Yet further prolonged treatment with RA and FGF4 still increases PDX1expression, but at this point cells could start to deteriorate, probably due to high confluence. Notably, the SB-705498 marked increase in cell number, but lack of effect on relative PDX1 expression, upon addition of FGF4 suggests that FGF4 primarily affect cell survival. Moreover, the cell viability assay AlamarBlue indicated that FGF4 promotes cell viability by reducing cytotoxic effects possibly exhibited by RA. Based on this observation we show that continuous treatment with RA and FGF4 after the AA-induction resulted in efficient induction of PDX1 mRNA expression. Immunofluorescence analysis was used to confirm that the observed increase in PDX1 mRNA expression was paralleled by a significant increase at the protein level. It should be noted that in cells that did not receive treatment with RA and FGF4, no PDX1-protein was detected. Efforts were made to passage the cells to new plates at this stage, but under currently used experimental conditions the cells failed to survive this treatment. The effect of RA and FGF4 was also evident by changes in cell morphology. Treatment with RA and FGF4 resulted in smaller cells that often were assembled in small cell clusters. To test the reproduci
it seems more likely that the effect of Pax4 expression on b-cell production is due to action later in the differentiation process
elial Infection by Lpn using an inverted BEEMH capsule, and sectioned en face. Sections were stained with lead citrate and viewed with the digital image acquisition system on the Jeol MEM-1011, transmission electron microscope. Cyanogenesis, a process by which cyanide is produced, has been demonstrated to occur in both bacteria and plants. Among bacteria, it has been studied extensively in fluorescent pseudomonads, especially Pseudomonas fluorescens and Pseudomonas aeruginosa. In addition to its activity in Pseudomonas, cyanogenesis has also been BMS 790052 chemical information reported in Chromobacterium violaceum and has often been reported to occur in the case of cyanobacteria such as Anacystis nidulans, Nostoc muscorum and Plectonema boryanum. Additionally, some strains of Rhizobium leguminosarum have also been reported to produce cyanide as free-living bacteria. In most of these cases, cyanide has been reported to be synthesized from the amino acid glycine. The sorption and mobility of cyanide produced from such organisms is mainly through soil surfaces and solutions. It is known that cyanide produced in the soil is usually associated with various metal ions, allowing for its rapid mobility in the ground water and subsequent diffusion in the atmosphere. The two most extensively studied bacteria for cyanogenesis, as stated earlier P. aeruginosa and P. fluorescens, are both commonly found in the soil. Pseudomonas aeruginosa, a Gram-negative bacterium, has been reported to cause opportunistic infections in humans, animals and plants. The ability of P. aeruginosa to infect humans and plants has been ascribed to its production of various secreted and surface associated virulence factors. Apart from various protein toxins, P. aeruginosa also produces small molecular toxins such as cyanide that facilitate the overall virulence of this opportunistic bacterium against multiple hosts. Interestingly, certain pseudomonads have been characterized as root colonizers of 26617966 several food crops that evade pathogenesis against multiple pathogens. Siderophores and cyanide production ability in various pseudomonads are linked to antagonistic and disease suppressing activity against various plant pathogens. The discovery that P. aeruginosa has the ability to infect plants allowed for the use of the A. thaliana – P. aeruginosa infection model by various research 2298299 groups. The two contrasting ecological roles of pseudomonads as a biocontrol agent and opportunist plant pathogen suggests that both root colonization and pathogenesis inflicted by this bacterium are highly species specific interactions. Apart from bacteria, plants have also been reported to produce cyanide to defend themselves against herbivores. In plants, cyanide is produced as cyanogenic glycosides and stored in the vacuole of the cell. Storage in the vacuole allows for separation from the enzymes, which act on the glycosides, and prevents the cells from auto-toxicity. When cells are damaged by herbivores, the cyanogenic glycosides and the enzymes are released from their separate compartments and react to produce HCN, which is toxic to herbivores. The potential of cyanogenic glycosides in plants as chemical defense has been demonstrated in Sorghum bicolor. Additionally, cyanogenesis plays an important role in the fitness of Trifolium repens against herbivores. The quantitative analysis of the amount of cyanide containing compounds stored in a given tissue and their capacity to release HCN per unit time as a reaction to herbivo
Membranes were developed using ECL Western blotting detection system according to manufacturer's protocol
and leaf plot and explore the duration of timing of the imatinib therapy versus progression or mixed response using logistic regression and Kaplan-Meir survival method. CD133 mRNA Detection We performed two-step Real-Time RT-PCR first performed the RT-PCR to generate cDNA from patients, which can be used in Real-Time PCR to detect CD133 expression levels in samples. RT-PCR was performed in a total volume of 20 ml containing 0.5 ml of oligo and hexamers, 1 mg RNA, 2 ml 106Buffer, and 25 mM MgCl2, 10 mM dNTPs, 0.1 DTT, 40 U RNaseOUT and DCEP-treated ddH2O. The thermal cycle conditions included 10 min at 25uC, 50 min at 45uC and 70uC 15 min, followed by 40 cycles of 95uC for 45 sec and 60uC for 1 min and 72uC for 1 min. Real-Time PCR was performed in total volume of 50 ml containing 25 ml 16 SyberGreen Mix-buffer, 1 ml Rox Refeence Dye, 6 mM MgCl2, 100 mM KCl, 400 mM dATP, dCTP, dGTP and 400 mM dUTP, 40 mM Tris-HCl, 6 mM300 nM each primers, 2 ml 23300835 cDNA template for each reaction, followed 95uC for 10 min and 45 cycles of 95uC for 45 min, 55uC for 1 min and 72uC 45 sec. All reagents used for PCR were purchased from Invetrogen, or Stratagene, and using Stratagene MX-3000 Real-time PCR System. Data interpretation: the amount of target, normalized to an endogenous reference and relative to the positive control is defined by the Ct method. The formula is applied as follows: Target amount = 22Ct, where Ct =. The assays were run on duplicates in two separate experiments and averages of the two experiments were then used in the final analysis. Results Study enrolled 24 evaluable GIST patients in 2003. Their peripheral blood mononuclear CD133 mRNA and plasma VEGF level were assayed via real time RT-PCR assay and sensitive ELISA respectively. Mean CD133 mRNA levels for GIST patients was 615.17. Mean VEGF levels was 107.42 pg/ml. The results showed that the relative amount of CD133 mRNA expression was significantly lower in the post-treated patients as compared to those same pretreated 7 patients. The VEGF value was also lower in the plasma of treated patients than that of untreated. The pre- and post treatment CD133 mRNA and VEGF levels were shown in VEGF Detection We used a complete kit for the quantitative determination of human VEGF in plasma of patients. We established standard series using a recombinant human VEGF standard. Wash ELISA microtiter plates once with dd-H2O, add 200 ml /well PBS:2% BSA, incubate 1 hour at 37uC, wash 6 times with dd-H2O, add 50 ml/well plasma diluted in PBS:1%BSA. Then incubate for 2 hr at 4uC, wash 9 times with dd-H2O, add 50 ml/well anti-human IgG:HRP diluted in PBS:1% BSA, incubate for 28 hr at 23964788 4uC, wash 6 times with dd-H2O, wash once with carbonate buffer, add 50 ml/well working substrate solution, 0.5 ml 4.0% OPD, 5 ml 30% H2O2, 1.0 ml 106 Substrate buffer, 8.5 ml dd-H2O. Incubate for 30 minutes at room temperature, add 25 ml/well 4.5N Sulfuric Acid. Read data at A450 nm by Packard SpectroCount and calculated to pg/ml for each sample by Packard I-Smart 2. The assays were run on duplicates in two separate experiments and averages of the two experiments were then used in the final analysis. Mean CD133 mRNA levels buy GSK343 Paired Differences p value Before After 6.702 2.369 P = 0.048 Mean VEGF levels Before After 134.117 55.174 p = 0.002 paired sample test doi:10.1371/journal.pone.0055520.t002 CD133 Correlates with Response to Treatment Data Items Two Indicators: CD133 and VEGF Correlation Sig. 0.183 0.917 0.258 Average Ratio
At this point, it is not yet clear which of the many SIRT1 substrates is/are responsible for the phenotype or which tissue share the metabolic defect
, in which cells display considerably more plasticity than fully differentiated cells, residing in a dynamic continuum between epithelial and mesenchymal states. One feature that characterizes metastable cells is that they display loose but intact cell-cell adhesions and show migratory properties in the form of collective movement as a sheet. Indeed, when grown to confluence, Py2T cells close a scratch wound as a cellular sheet. A further indicator for a metastable state is the observation that, when grown under sparse culture conditions on plastic, Py2T cells are able to transiently leave the epithelial sheet and move as single cells in a spontaneous manner. This single cell mode of migration resembles amoeboid movement, characterized by a rounding of cell bodies and a fast change in direction, and is distinct from the mesenchymal mode of migration characterized by front-rear polarity which we observed with Py2T LT cells . The reversibility of TGFb-induced EMT of Py2T cells further illustrates the plasticity of Py2T cells and has also been proposed as a hallmark of metastability . From these observations we conclude that cultured Py2T cells do not represent fully differentiated epithelial cells, but that they are rather in a metastable state that is readily shifted towards a mesenchymal phenotype by TGFb treatment. When implanted into the mammary fat pad microenvironment, Py2T cells eventually develop tumors with an EMT-like phenotype. We believe that the term "EMT-like�� is accurate, since we have noticed that in these tumors, Py2T 22880633 cells do not completely convert into mesenchymal cells as they do under culture conditions in the presence of TGFb. Breast cancers can display a range of DCC-2036 stages of EMT, in fact, tumor-associated EMT appears 15647369 less complete than developmental EMT. A staging scheme has been proposed based on the state of cell polarization, cell cohesiveness and intermediate filament expression, categorizing oncogenic EMT into four distinct stages, with P0 designating full epithelial differentiation and P3 indicating a fully mesenchymal state. Py2T tumors correspond to the P2 stage, where cells have lost polarization and cohesive cell-cell contacts, but retain cytokeratin expression and fail to upregulate vimentin. When we block TGFb-responsiveness in Py2T cells, epithelial morphology is retained in distinct areas, where tumor cells appear to be organized as dynamic cohesive sheets or strand-like structures, however not regaining full epithelial polarization. This phenotype is again consistent with a metastable state rather than full epithelial differentiation, and corresponds to the P1 stage of oncogenic EMT according to. Despite the fact that Py2T cells form locally invasive tumors and that MMTV-PyMT tumors give rise to distant metastases, we were unable to detect any apparent metastases evoked by Py2T tumors. One conceivable reason for this apparent discrepancy Py2T EMT Model could be the following: Py2T tumors appeared fast growing and aggressive and, due to animal welfare considerations, mice had to be sacrificed approximately 25 days after implantation. Therefore, the timeframe to establish detectable metastasis may be simply too short. In comparison, the metastasis latency in MMTV-PyMT tumors is about 3.5 months. We have observed that PyMT transgene expression is absent in Py2T cells both in vitro and in vivo. This finding has important implications. First, it allows the transplantation of Py2T cells into syngeneic FVB/N
While extreme levels of sequence divergence do not seem to compromise the accuracy of the LRTs, the Bayesian prediction becomes unreliable
onsible for severe diseases in humans and animals including intestinal or foodborne diseases as well as gangrenes. Individual strains produce only subsets of toxins and are classically divided into five toxinotypes based on their ability to synthesize Alpha, Beta, Epsilon and Iota toxins. Delta toxin is one of the three hemolysins released by a number of C. perfringens type C and also possibly type B strains. This toxin was purified from a C. perfringens type C strain and characterized as a basic 42 kDa protein which Chlorphenoxamine biological activity specifically hemolyzes erythrocytes from even-toed ungulates . It was further showed that Delta toxin is cytotoxic for other cell types such as rabbit macrophages, human monocytes, and blood platelets from goat, rabbit, human and guinea pig. The selective cytotoxicity of Delta toxin was correlated to a specific binding to the ganglioside GM2. Indeed, the hemolytic activity of Delta toxin as well as the binding of iodinated toxin to target erythrocytes is preferentially inhibited by GM2. In addition, iodinated Delta toxin was shown to specifically bind to ganglioside GM2 extracted from membrane of sensitive cells and to liposome containing GM2. Thus Delta toxin was revealed to be an excellent tool for probing GM2 on cell membranes. In addition, Delta toxin selectively lyses malignant cells expressing GM2, such as carcinoma Me180, melanoma A375, and neuroblastoma C1300, and in vivo administration of Delta toxin to mice bearing these tumors significantly reduces tumor growth. However, the mechanism of cytotoxicity remains unclear, since Delta toxin was reported to not insert into cell membrane and to induce membrane lysis by an unknown process. To further study the cytolytic mechanism of this toxin, we have cloned and produced a recombinant protein fully active on target red blood cells and which retains the binding to GM2. Here we report the molecular characterization and pore forming activity of the recombinant C. perfringens Delta toxin in lipid bilayer experiments in comparison with C. perfringens Beta toxin and Staphylococcus aureus alpha toxin, two well established pore-forming C. perfringens Delta Toxin N-terminal Peak 16 Peak 18 P723 NDLGSKSEIRKE EINSYHIAXDTEXQG DGYNVNSWNIVYGNQMF AATTCITGGAATATIGTITATGGIAATCAAATGTT 10980276 A 1,6 kb DNA fragment was amplified and cloned. DNA inserts of the two recombinant plasmids pMRP680 and pMRP980 were sequenced and assembled, and the 3359 bp DNA fragment containing the whole Delta toxin gene is shown in Features of the Delta toxin gene The open reading frame from nucleotide 1048 to 2004 recognized by the probe P723 deduced from the Delta internal sequence was assigned to Delta toxin gene. A consensus ribosome binding site, GGGGTG, is located 7 nucleotides upstream of 2436504 the initiation ATG codon. An inverted repeat of 15 nucleotides separated by 3 nucleotides and able to form a stem loop structure was identified 52 nucleotides downstream of the stop codon. This structure corresponds to a putative transcription terminator. Downstream of cpd lies an open reading frame which is transcribed in the same orientation as cpd. Orfx2 encodes for a short basic protein of 198 aa. At the amino acid level, Orfx2 shows significant identity level and similarity with transposase from various bacteria such as IS650/IS653 from Bacillus holodurans, IS116/IS110/IS902 family from Desulfotomaculum reducens, Thermoanaerobacter tencogensis, Fervidobacterium nodosum, Clostridium kluveyri, and Clostridium cellulolyticum,
The evidence for functional F-box domains is available for both A. tumefaciens and R. solanacearum F-box containing proteins
3s Ser69/74Ala protein on the Smad3-dependent transcription, its overexpression significantly inhibits the putative CSC population even without disturbing the Smad3-dependent transcription. This observation can potentially suggest that abrogation of TGFb1 dependent phosphorylation of 14-3-3s at two sites can trigger the Smad3 independent 16494499 mechanisms of CSC regulation by TGFb1 axis. Tumorigenic properties of MCF7 cells stably expressing wild type and the mutant 14-3-3s proteins were analyzed in vivo using NOD/SCID mouse xenograft models. Our data indicates that the MCF7 xenograft tumors expressing 14-3-3s Ser74Ala and 14-33s Ser69/74Ala mutant proteins showed statistically significant decrease in the growth rate in vivo compared with a control group. Analysis of cell apoptosis in the xenograft tumors using TUNEL staining confirmed a higher number of apoptotic cells in the MCF7 xenograft tumors expressing 14-3-3s Ser74Ala mutant protein. This result is in agreement with in vitro data for TGFb dependent inhibition of sphere formation and suggests that phosphorylation of 14-3-3s at Ser69 plays an important role in cell tumorigenicity and survival. In breast cancers, a subset of the cells with high aldehyde dehydrogenase activity and CD44+/CD242/low pheno- 15 Phosphoproteomics of TGFb1 Signaling type has been characterized as CSCs population with tumorigenic and radioresistant phenotype,. Histological analysis of MCF7 xenograft tumors expressing 14-3-3s wild type or Ser74Ala mutant proteins revealed a decrease in CD44+/CD242/low cell phenotype as compared with a control group or with the MCF7 xenograft tumors expressing 14-3-3s Ser69Ala or double mutant proteins. As suggested by preclinical studies and clinical observation, resistance to conventional therapy, including irradiation, has been reported to be a defining characteristic of CSCs from various tumor types, including breast cancer. One of the bestcharacterized chromatin modification events in responses to cell irradiation is histone H2A.X phosphorylation by ATM or ATR serine/threonine protein kinase. The phosphorylated form of H2A.X forms nuclear foci on the sites of DNA double strand breaks 17984313 and the number of c-H2AX foci approximates the number of DSBs induced. Recent studies demonstrated that in murine mammary gland CSCs, c-H2A.X foci resolved faster than in non-CSC population that perhaps is reflective of more efficient DSB repair in CSCs. Immunofluorescent detection of phosphorylated c-H2A.X revealed more residual DNA damage at 4 hours after irradiation in the cell overexpressing 14-3-3s Ser74Ala mutant protein and treated with TGFb as compared to the control cells. Because successful DNA reparation in the irradiated cells is associated with their survival, we further assessed the JNJ-7777120 web surviving fraction of the cells treated or non-treated with TGFb and irradiated with 4 Gy X-ray dose. Our data suggest that activation of TGFb signaling pathway decreases cell survival after irradiation and, besides of that, surviving fraction of the cells overexpressing 14-3-3s Ser74Ala mutant protein was significantly reduced as compared to the control cells. These results showed an impaired repair capability in the cells overexpressing 14-3-3s Ser74Ala mutant after TGFb treatment, which is consistent with the decreased cell survival. Preclinical assays and clinical findings suggest that a higher proportion of tumor initiating cells correlates with higher tumor radioresistance. ALDH activity selectively


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Getting ready for the holidays is always super stressful, and it's not going to get any easier this year with a pandemic. That doesn't mean holiday planning is impossible though. Here are a few ways you can have a safe, fun holiday season.
The first thing you'll want to do is get your holiday shopping done early. There's nothing worse than realizing you forgot to get a gift for your mother-in-law or neighbor last minute. Make a list of everyone you need to go shopping for, and check everyone off as quick as you can. You'll feel better knowing you won't need to run around the mall like a chicken with it's head cut off on Christmas Eve.
Another good idea is to decide beforehand how many holiday events you'll want to attend this year. This may be a difficult choice to make, but the last thing you want is to get covid-19, or even just the flu. If a family event involves traveling, take that into consideration before you decide to go. Will you have to fly, or can you drive instead? Do you have a few extra face masks to take on your trip? You may also want to find out how many people are expected to attend, and if social distancing will be encouraged. None of this is to discourage you from seeing loved ones, but to help you stay safe and healthy. With flu season coming up, it's more important than ever to stay well. Ask your family and friends to help you stay healthy by social distancing and wearing face masks at gatherings. That way you can see everyone and be safe.
You'll also want to send out holiday cards. With all the social distancing going on, you may not have had as many opportunities to see friends and family this past year. Sending out a card is an easy way to update everyone on how you and yours have been doing with everything going on. Your loved ones will be so happy to get an update. You can find great holiday card designs from Basic Invite. Go check them out!
Finally, be sure to spend some time this holiday season practicing self-care. This past year has been hard on all of us, meaning we all need to take some time for ourselves. Go for a walk or a run, meditate, or spend a few minutes each day practicing gratitude. Spend time focusing on the present moment, instead of getting caught up in the past or future. If you get stressed out, remember to take a few deep, calming breaths. If you use one or two of these techniques throughout the upcoming holiday season, you'll be much better off and less worried about things. Taking care of your mental health is just as important as taking care of your physical health.
Although we're entering a stressful time of year, you can address your worries and make life easier on yourself. Remember to take care, get your shopping done early, send out holiday cards, and be safe while attending events. Happy holidays!

subset: commoncrawl


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Anyway, The Lemon Twigs were electric. Amazing. And truly unpredicactable. The two brothers leading the band would jump around instruments, and the younger sibling came across as a balance of Keith Moon and some outer space glam alien. I had no idea what to expect but their mist cure of brawny rock, twisting hooks, theatricality and purse physicality won me over. By the end of the set the group had won over at least two more fans for life.
Their recorded music varies in quality, so while I enjoy their albums, none of them capture just how wild and loud The Lemon Twigs are IRL, but yesterday they released The Lemon Twigs LIVE, a live album stitched together from performances on their latest 2018-2019 tour. And I am so happy to finally have a reasonable document of the group's onstage power. Even better, The Lemon Twigs are donating 100% of proceeds from sales of the album to Coalition for the Homeless.*
Give it a listen and buy it! I should also note, that if you wait to buy The Lemon Twigs LIVE until tomorrow, Bandcamp is waiving all their fees again on May 1 to support the artists on the platform, so even more of your cash will go to charity.
*I'm really enjoying bands releasing live benefit albums right now. Everybody wins! The first I bought was from Father John Misty near the beginning of our unified lockdown, so I think it's super funny there's a long Father John Misty story told during "Hi+Lo" on this album.


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It really is a simple prediction to make. The liberals (elected, media and blogger) have so over-played the whole Cheney shooting debace that it will backfire on them just like the Paul Wellstone funeral.
TIME is trumpeting as loudly as they can that Cheney is at 29 points. But the poll has some even more valuable data to my prediction:
7-in-10 (69%) Say Shooting Incident Has Had 'No Effect' on Their Overall Opinion of the Vice President; About One-Fourth (24%) Say it Has Had a 'More Negative' Effect
So 93% of the people who responded either had no opion change or they went negative. So when the bump happens it will be indisputable the the bumb came from the over-coverage and not the event.
Wait for it.


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Best Men's Devotional Bibles
What are some good Men's Devotional Bibles? Here are some of the best, covering a range of Bible translations. They are all a little different, so give these a look and see if one of them works for you.
Best Men's Devotional Bibles (Updated List)
Preview | Product | Price |
Men of Character Bible Holman Bible Publishers 2021 Christian Standard Bible Jump to Details | Check Current Price ChristianBook.com Amazon.com | |
Men's Devotional Bible Crossway 2015 English Standard Version Jump to Details | Check Current Price ChristianBook.com Amazon.com | |
Every Man's Bible Tyndale House Publishers 2014 New Living Translation Jump to Details | Check Current Price ChristianBook.com Amazon.com | |
Outdoorsman Bible Zondervan 2019 New International Version Jump to Details | Check Current Price ChristianBook.com Amazon.com |
What Is a Men's Devotional Bible?
Devotional Bibles serve a different purpose than Reference Bibles and Study Bibles. Devotional Bibles contain articles and devotions intended to focus the reader on applying Biblical principles to their daily lives. They may include inspirational stories with thought-provoking questions and quotations relative to a particular passage of Scripture.
Men's Devotional Bible are designed to bring both encouragement and insight into the issues men face every day. They differ from study Bibles in that they generally do not present theological or doctrinal positions. While they may include cross-references, a concordance or similar study helps, the primary focus is on practical, everyday living.
What to Consider in Men's Devotional Bibles
Everyone has different preferences on what is important or not when deciding on a Bible. Here are some key things a Men's Devotional Bible should have.
The Bible Translation
Bible translations range from very literal to broadly paraphrased. The former can often be difficult and awkward to read. The latter is highly subject to the editor's doctrinal preferences. Choose a translation that is faithful to the original text and yet easy to comprehend. If you have questions about a translation, this page compares a few verses in different versions.
Presentation Style
Bibles come in a variety of presentation styles. Both single column and two-column page layouts are common. Sometimes there are cross-references along the side or down the middle of a two-column layout. The text itself might be divided into individual verses or formatted in paragraphs. Choose the layout that is easiest to read. Check the "See More Details" link for each Bible below to see if a sample page is available to view.
Physical Characteristics
A Bible that has very small print or unusual font styles might be difficult to read. A Bible that is very large or heavy might be cumbersome, but one that is very small might be uncomfortable to handle. Do you want a softcover or hardcover? Consider whether or not a "red-letter edition" might be distracting or unduly influence what is being read. Check the "See More Details" link for each Bible below to see the different options available.
Men's Devotional Bibles Product Reviews
Here are brief overviews of the best Men's Devotional Bibles.
Men of Character Bible
From the Publisher:
Our world is filled with powerful and influential men, but men who aspire to reflect the characteristics of God are what our chaotic world desperately needs. In the CSB Men of Character Bible, 60 men of the Bible are featured as examples of virtuous men who brought power, wisdom, and inspiration to God's people. Each of these men faced trials, frustrations, and failure, yet they were inspired and strengthened by God to achieve great goals and emerge victoriously.
- 60 in-depth articles on men of character in the Bible
- More that 45 profiles of men of character in the Bible
- 14 Measure of a Man articles
- Book Introductions with Timelines
- CSB Concordance and Color Maps
- Specific plans for "How to Use This Bible"
- Bible reading plans (undated)
- Two-column Bible text
- Paragraph format
- Black letter text
- Side column cross references
- 10 point type for Bible text
Bible Translation: Christian Standard Bible (Holman Bible Publishers)
See More Details >Men's Devotional Bible
From the Publisher:
With 365 theologically rich and gospel-centered devotions drawn from God's Word, this all-new resource was created under the editorial oversight of Dr. Sam Storms with contributions from over fifty Christian leaders. Introductions orient men to each book of the Bible, exploring its unique contribution to our walk with God. Thoughtful and instructive articles address the importance of sound doctrine, life in the local church, leadership, the heart, calling, and a host of other relevant issues for today.
- 365 devotions, undated, enough for one year
- 13 articles for men
- Dictionary of key terms
- Two-column Bible text
- Paragraph format
- Black letter text
- 8 point type for Bible text
Bible Text: English Standard Version (Crossway/Good News Publishers)
See More Details >Every Man's Bible
From the Publisher:
The Bible for every battle every man faces! This is a man's type of Bible—straight talk about the challenges of life. Every Man's Bible has thousands of notes on topics from work, sex, and competition to integrity and more and trusted advice from the pros, just for men. Every Man's Bible is written by the best-selling author of the Every Man's series, Steve Arterburn.
- Feature articles on relationships
- Profiles of men in the Bible
- Advice for men from well-known Christian teachers
- Book introductions in detailed outline form
- How to Lead a Men's Bible Study
- One year Bible reading plan
- Two-column Bible text
- Paragraph format
- Black letter text
- 7 point type for Bible text
Bible Text: New Living Translation (Tyndale House Publishers)
Also available in the New International Version
See More Details >Outdoorsman Bible
From the Publisher:
For the man with a passion for field-ready gear, the NIV Outdoorsman Bible includes devotions that tie directly to the sports of hunting, fishing, backpacking, or any outdoor activity. This complete NIV Bible includes full-color inserts graphically designed for the sportsman's eye. As a one-stop destination for any outdoorsman who studies the Bible, each of these unique pages includes a personal devotion, as well as related material for small group discussion.
- 26 outdoor-themed articles for spiritual reflection
- Lost Camo® cover is designed to lay flat
- Part of every purchase goes to LOST Camo®, which supports missionaries around the world
- Two-column Bible text
- Paragraph format
- Red letter text (Words of Christ in red)
- 9.5 point Bible text
Bible Text: New International Version (Zondervan)
See More Details >


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On March 3, the Mijia Electronic Thermometer went on sale again. It is focusing on fast and accurate temperature measurement. The temperature measurement accuracy can reach ± 0.05 degrees Celsius, and the price is only 69 yuan ($10).
Also Read: Mijia Electronic Thermometer Announced At 69 Yuan ($10)
The Mijia Electronic Thermometer supports two temperature measurement modes. The 30-second speed measurement allows you to quickly obtain the temperature value. After the rapid temperature measurement is completed, do not remove the thermometer. Continue to measure the temperature for about 5 minutes until the buzzer issues a reminder again.
This thermometer is equipped with an LCD display and designed with backlight function, which can easily read the temperature value even at night.
The Mijia electronic thermometer supports Bluetooth 4.2. After connecting to the Mijia app, the temperature measurement progress and temperature values can be displayed in real time. When the body temperature reaches or exceeds a specific temperature, the background of the Mijia electronic thermometer plug-in will automatically change color to remind high temperature.
In the Mijia app, you can add 9 family members. You can choose which family member to take the temperature by turning on the temperature timer. You can intuitively display the family's temperature records through the temperature curve in the app to establish a temperature history file for the family.
In terms of measurement accuracy, the Mijia electronic thermometer uses a high-precision NTC temperature sensor. As said, the measurement accuracy can reach ± 0.05 degrees Celsius in the common range of 35-39 degrees Celsius.
It is worth mentioning that the Mijia electronic thermometer uses a unique smart storage box design. The thermometer will automatically turn on when it is unplugged. After the temperature measurement is completed, the thermometer box will be automatically turned off.


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LVMarkets Review by professional Forex trading experts the "ForexSQ" FX trading team, Finding out everything you need to know about the broker is in this LVMarkets Review.
LVMarkets Review
LVMarkets is a broker that offers trading on forex and CFDs on oil and indices on one type of live trading account – Standard. Its spreads are fixed and it does not charge commissions on forex trading. There is also a free demo account for those who wish to try trading without risking their own funds.
The Company. Security of Funds
Company Country Regulation
LVMarkets Vanuatu None
LVMarkets was established in 2016 and is based in the offshore zone Vanuatu. The broker's site displays a Dealers in securities license from the country. Vanuatu, however is an offshore zone and they are notorious for its lax requirements and laissez-faire approach.
It is much safer to use the services of a forex broker supervised by a much more reputable regulator like UK's FCA or the Australian Seucirties and Investment Commission.
Trading platforms
Like most brokers LVMarkets offers MetaTrader 4 as a trading platform. Usually it is the most obvious choice for the forex brokers, as it is user friendly and has many options. Besides, most traders are well familiar with it. The MT4 has charting and technical analysis tools and allows hedging and auto trading.
Methods of payment
We could not find any information on the ways in which a client can make a deposit to their trading account.
LVMarkets is a new unregulated offshore forex broker. Its spreads are in the average in the minimum deposit requirement is decent, compared to other similar brokers who require $1000, for example, but its leverage is low.
We did not like the fact that there is barely any practical information on the website, for example on the asset portfolio or the funding and withdrawal options.
As a general rule doing business with unregulated offshore forex brokers is a very bad idea and the potential investors should rather choose a forex broker regulated by a watchdog of higher reputation. More often than not it would offer better conditions in addition to the regulatory protection.
The Broker is not a safe forex broker, Instead you can use Plus500, XM, eToro, Fxpro or AvaTrade forex brokers.
LVMarkets Review Conclusion
For more information about currency trading brokers visit TopForexBrokers.com forex brokers comparison website, Tip ForexSQ.com foreign exchange trading experts please by share this article about LVMarkets Review.


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Mar 13, 2022
Another enlightening and jam packed conversation with my 16 year-old niece, Sienna Krieg. In this Good Karma Success Coach podcast episode we recap her Soul Introduction Reading that she had with my good friend Debbra Lupien- The Voice of the Akashic Records. I gifted Sienna a reading for her 16th birthday and she shares her learnings and how they will help her in her life.
In addition to her reading with Debbra, we also talk about Human Design which is something I encompass in my coaching and embody.
Also we discuss Medium.com which Sienna started writing on the day before her birthday. She has now crossed 100 followers, which is very exciting.
Links to connect with both Debbra and Sienna below:
Interested in Medium? My affiliate link is below:
Other young adult writers on Medium.com that Sienna mentions:
Please follow and support them too!
Sienna has been on episodes:
144, 151, 155, 160, 167, 178 and my "why" for cohosting with Sienna is episode 143.
Debbra Lupien has recently been on episodes 172 and 173 talking about meditation and her three best selling books which are on Amazon.
All other info below.
Thank you for listening and sharing!
Melinda Van Fleet is a Sales Strategy & Success Coach | Consultant who helps businesswomen make sales and feel good. Life Coach for female entrepreneurs. Mindset and tactical tips for business and life success- Human Design, intuition, and confidence building.
Both bestselling books (Confidence Mastery for Couples and Life & Love Lessons) on Amazon (Paperback, eBook and Audible) or connect through website:
Check Out These Fast Paced Transformation Courses:
Supercharge Your Transformation! Own Your Personal Power and Success Now!
Medium.com Excellerant Course
Private FB Group https://www.facebook.com/groups/beyondbasic
Connect on FB, LI, YouTube, Twitter, Pinterest, or IG melinda_vanfleet
Medium Publication is Beyond Basic.
Two Weekly podcasts:
Are you ready for private 1:1 coaching?
Email me at email@example.com
And/or check out the link below for more info:


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Nepal Telecom subscriber base reaches 5.45 million
Government-owned Nepal Telecom (NT) has added 1.2 million subscriber in the current financial year. NT has added around 100,000 subscribers per month, said Amar Nath Singh, Managing Director at a press conference today.
NT's subscriber network has expanded to over 5.45 million consumers. GSM has contributed 72.66 per cent to the NT network with 3.96 subscribers, followed by 9,05,000 CDMA subscribers and 5,84,000 PSTN subscribers.
Rapid growth of GSM has made us the number one telecom service provider in the country, he said. NT is planning to add 5 million subscribers in the next three years to remain in the top position. It is a challenge for us but we are to compete in the market, he said.
"NT will go rural to expand its network base in the coming years," he said. The company has planned to add 1.8 million villagers through GSM mobile in the next two years. The company is also planning to add 600,000 subscribers in for the CDMA.
Likewise, NT is adding 3.5 million mobiles lines having modern facilities like 3G/4G services. "We are analysing technical parts of the system," he said. NT is planning to develop 'interruption free GSM service' along east-west highway, Kathmandu-Pokhara highway and other highways linking north to south.
"We want to provide interruption free GSM service in major highways next year," he said. Moreover, NT is providing WiFi services in 500 public places next year. This is to improve the brand image of NT. Such places can be hospitals, bus stations, colleges, etc., he said.
"Such places will be selected as per maximum benefit to the people," he said. NT governing board has approved Rs 48.7 billion budget for 2010-11. Of this, NT has a reserve of Rs 44.20 billion while the remaining amount will come through other sources. NT has posted Rs 10.17 billion profit this year and has paid over Rs 15 billion in revenue. (Source : THT)


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Created from the Seoul & The Happy Unicorn Children's Boutique
Regular price $ 3.75
All orders over $75 ship for free (cooling packaging and gift wrap does is not applicable)-Don't forget to sign up for our rewards program & we accept Sezzle !
Place a safe amount of wax melts/tarts into your warmer. Turn on or light your warmer and enjoy the amazing aroma that it gives.
Our wax melts are handmade in small batches. We mix our Parasoy wax with safe fragrances, dyes/micas. & glitter.
Please see warning label in the pictures. Please note that shipping wax melts in the heat can cause damage to the product. We are not responsible for the damage made in the shipping process. We do have options that include shipping with moisture free reusable cold packs & cool shield packaging for an additional cost. Purchase here:
MTO: Made to order. Typically ships within 2-4 business days.
RTS: Ready to Ship: Typically ships within 1-2 business days
WHOLESALE: Message me on Facebook at: www.facebook.com/iloveswt
Gift Wrapping is available: https://the-happy-unicorn-childrens-boutique.myshopify.com/collections/wax-melts-tarrs-scoopable-wax/products/gift-wrap-option


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The Embalse nuclear power plant in Argentina resumed commercial operation following a three-year upgrade programme that was completed in January, the country's Ministry of Energy announced on 30 May. Plant operator Nucleoelectrica Argentina has been conducting tests in the four months since first criticality.
The plant was shut down at the end of 2015 for the modernisation work, which included reactor retubing and replacing the steam generators. It also included a 6% increase of the plant's capacity to 683 MWe. Embalseentered commercial operation in 1984 and is now able to continue operation for another 30 years,
Argentina has three operating nuclear power plants, all pressurised heavy water reactors. With total generating capacity of 1627 MWe, the three units - Atucha 1 and 2 plus Embalse - provide about 10% of the country's electricity.
Located in the province of C��rdoba, the Embalse plant achieved a first controlled nuclear chain reaction, also known as criticality, on 4 January. It is the third Candu 6 reactor to undergo a full refurbishment, after Wolsong 1 in South Korea and Point Lepreau in Canada.


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Rivals Merging Together
One of the oddest things the COVID-19 pandemic has spawned is the strange partnership between rivals Apple and Google. The two tech giants are teaming up to help track the virus.
The Collective WHY…
Apple is developing a feature to embed in iPhones while Google came up with an Android device that enables users to track infected people.
The technology giants, who rarely pass up the chance to trash each other, are now holding hands and singing 'Kumbaya'.
Subsequently, they are uniting to slow the spread of the virus. This merger can help with curbing a resurgence of COVID forecasted in late fall. Published reports state that the two tech giants are within months of completing the operating system. Eventually, this will link billions of iPhones and Android devices. Click Here to view original article.
CNBC Article states "Google's site says the apps won't collect personally identifiable information or user location data and people who test positive are not identified to other users Google or Apple." Apps will store location for about 14 days. The data will "only be used for contact tracing by public health authorities for COVID-19 pandemic management."
More importantly, this is opt-in contract tracing. That means users will need to agree to be tracked if they want to participate. Contact tracing can allow health officials to monitor the spread of coronavirus by seeing who has been in close contact with infected people.
How the Smartphone Technology Works
Overall, devices will only be enabled when a user opts-in. Once this feature is selected, it will interface with similar devices. These efforts focus on further identifying those that test positive.
The final step requires mobile users to voluntarily report that they are infected. Public health officials across the globe are anxiously awaiting this advance. At this point, they are open to any solutions to contain the pandemic.
However, only a few others are excited about the two largest tech companies in the world working together. Whispers of a worldwide monopoly are omnipresent. Imagine the power of two companies who virtually own the most personal information for just about everyone on the planet.
Although a useful tool, the over-reach into individual privacy is somewhat unsettling. This transition will take it to the next level. Imagine the consequences of an evil mastermind who is capable of subverting computer security for malicious purposes. Lets hope this doesn't bring on something far more terrifying than COVID-19.


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The Golden Globes are technically about recognizing talent in film and television, sure, but we often don't recall the annual show by who won which awards. For example, we'll remember this year's as the one where a swath of old men inexplicably wore transition lenses. Where women let their boobs out, to glorious effect. And where a handful of couples — rumored and confirmed alike — walked the red carpet together, either for the first time, or one of them.
One such couple was actress Beanie Feldstein, 26, of Lady Bird and Booksmart, and her girlfriend of a little over a year, film producer Bonnie Chance Roberts. While the pair technically made their red-carpet debut at the premiere of Cats in New York City last month, they drew quite a bit of attention at the Globes last night, when many learned of their relationship for the first time. And their appearance was quite endearing: Roberts wore a collar clip that matched Feldstein's dress, which fans responded to on social media with lots of hearts and crying emoji.
Here's what we know about them.
Roberts is a Scouser.
That's apparently what you call people from Liverpool, which is where Roberts was born. According to her bio on the London Screenwriters' Festival's website, Roberts graduated from Birmingham University in 2013, and "was lucky enough to land her first job as assistant to producer Debra Hayward," who produced Les Misérables. Neat!
She and Feldstein met on the set of How to Build a Girl.
Roberts worked as a film producer for the 2019 film adaptation of author Caitlin Moran's How to Build a Girl, where she and Feldstein first met. (Feldstein had the starring role.) While we don't know how exactly the two found themselves drawn to one another, there must have been a pretty strong connection up front, as Feldstein told Teen Vogue that she wasn't much of a relationship person before meeting Roberts. When they started to date, she says, she "[got] why people write songs."
"Not to sound flippant, but I was in love with her and all of her, and she's a woman," Feldstein said. "That's not scaring me or deterring me. And it wasn't just women in general — it was her specifically."
Roberts is "the best person" Feldstein has ever met.
Or so she says on Instagram, where the two have been posting photos of each other for well over a year now. In a photo Roberts uploaded of them together in August 2018, they're huddled together with another woman — which is to say that, at the time, you probably couldn't have gleaned the nature of their relationship from the photo. But you can assume that they were dating by October of that year, when Feldstein uploaded a more intimate photo of them with the caption, "She made it across the pond."
Ever since then, they've been all over each other's Instagrams.
To be young and in love!!!


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Kings vs. Grizzlies Prediction
Will the Memphis Grizzlies find a way to get back on track when they play host to the Sacramento Kings tonight at 8PM ET?
535 Sacramento Kings vs. 536 Memphis Grizzlies
Friday, February 28, 2020
8PM ET – FedEx Forum
Compare odds and from multiple different online sportsbooks and shop for the best lines at our new NBA Odds & Betting Page.
Public Betting Trends
According to the latest oddsmakers, the Grizzlies are the slim favorite at home, as they are getting odds of -4 points against the Kings. The over/under total for the contest has yet to be listed. The public betting currently is unavailable for this game right now as well. This information is subject to change, however, so be sure to check out our NBA Public Betting Page for updated figures on the game.
Kings end win streak
Sacramento is coming off a 112-108 loss at Oklahoma City last night to snap a three-game win streak. However, the Kings have covered the spread in four straight games. Sacramento is now 24-34 on the season, 12-19 on the road, 30-27-1 against the spread and 30-28 with the over.
The Kings are putting up 108.4 points per game on offense this season while shooting 45.8 percent from the field. Defensively, Sacramento is allowing 110.6 points per game while opponents shoot 46.8 percent against them. De'Aaron Fox is questionable to play tonight due to an abdomen injury. He didn't play last night but is optimistic about today. Fox is averaging 19.9 points and 6.9 assists per game. Buddy Hield is adding 20.1 points and 4.9 rebounds per contest.
Memphis returns home
Memphis wrapped a four-game road trip where it lost and failed to cover in all four games. The Grizzlies are coming off a 140-112 loss at Houston on Wednesday. Memphis is 28-30 on the season, 16-13 at home, 30-27-1 against the spread and 29-29 with the over/under total.
The Grizzlies are giving up 115.3 points per game on defense this season while opponents shoot 45.7 percent against them. Offensively, Memphis is averaging 112.8 points per game while shooting 47 percent as a team. The Grizz remain without Jaren Jackson Jr. due to a knee injury. Jackson averages 16.9 points and 4.7 rebounds per game. Ja Morant is leading the team with 17.5 points and 6.8 assists per game. Dillon Brooks adds 15.3 points per contest.
NBA Betting Trends
The Kings are 6-2 against the spread in their last eight road games and 4-1 ATS in their last five games following a loss.
The Grizzlies are 7-1 against the spread in their last eight games as a favorite and 4-0 ATS in their last four home games.
The home team have won and covered in all three meetings this season and I like that to happen again tonight. The Kings are playing well as of late, while the Grizzlies are playing poorly, but Sacramento played last night and is on its third road game in four nights. Memphis has just one home game over their last eight, which was a win over the Blazers. The Grizz are still solid at home and still playing for a playoff spot with a young roster. I expect Morant to raise his game tonight and help Memphis snap out of its funk.
NBA Prediction: Memphis Grizzlies -4


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Toonami Announces Attack On Titan Marathon
Earlier this year, Attack on Titan gave fans a long-awaited gift when its second season finally [...]
Earlier this year, Attack on Titan gave fans a long-awaited gift when its second season finally debuted. The critically acclaimed anime wrapped its first season back in 2013 before an extended hiatus delayed its comeback more than once. Otakus have been able to enjoy Attack on Titan's new stories since April, but the series only made it to US television recently thanks to Toonami. And, now, the late-night block is trying to help fans catch up with the series.
If you don't already have plans for the July 4th weekend, then you may want to spend it with Toonami. The program announced today that it would be hosting an Attack on Titan marathon of season two for the holiday weekend.
"BBQ, fireworks, and titans! Kick off your Fourth of July weekend with an Attack on Titan marathon. On July 1, we're showing episodes 26 – 34 from 11pm – 3:30a!" Toonami declared on its Facebook page.
The marathon will cover season two up until the anime's 34th episode. The last episode, which is titled "Opening," will follow Eren after the hero finds himself kidnapped by some unlikely suspects.
You can read the synopsis of Attack on Titan's new season below:
Eren Jaeger swore to wipe out every last Titan, but in a battle for his life he wound up becoming the thing he hates most. With his new powers, he fights for humanity's freedom facing the monsters that threaten his home. After a bittersweet victory against the Female Titan, Eren finds no time to rest—a horde of Titans is approaching Wall Rose and the battle for humanity continues!
If you are not familiar with Attack on Titan, then you should know the popular anime is renowned for its intense action and gritty story lines. The anime follows a boy named Eren Yeager in an alternate world overrun by massive creatures known as Titans.
These mindless beasts have all but wiped out humanity, and mankind is left to cower in fear behind massive walls. When their sanctum is infiltrated by a slew of Titans, Eren and his friends Armin and Mikasa join the military to help eradicate the blood-thirty monsters. But, as they grow older, they learn that the beasts are not what they appear as long-held government conspiracies make themselves known.
[HT] Facebook


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SUPERIOR — The state's top tree climbers know that most people probably have a few misconceptions about their sport.
"They're going to think we're weird or we're tree huggers or something like that but no, we're athletes," Jon Bora, owner of All-American Arborists, said.
Bora was one of several tree trimmers and arborists that made the trek to Boyce Thompson Arboretum in the Tonto National Forest Saturday for the Arizona Community Tree Council's annual tree climbing championship. The contestants tested their skills in a number of different challenges in the morning that replicated what they would do in their day jobs as tree trimmers — without the chainsaws.
"We're not cutting or doing any harm to the trees," Jesse Huffman, owner of Arborvision Tree Service, said. "It's non-invasive tree climbing to mimic what we do on the job everyday."
Three arborists advanced to the master's challenge, the ultimate competition that combined elements from all the morning's activities.
Andy Young, who owns a tree service in Mesa called The Tree Amigos, dominated the competition, winning his second straight state title. He chalked it up to his early start in the sport.
"From a young age, I was climbing door jams, climbing the refrigerator," Young said. "I'd climb anything I could, not knowing that it might be my occupation when I got older."
All three of the finalists said the competition was mostly about refining their skills in a competitive environment, with the end goal of doing their day jobs better and more safely.
"Coming out here and learning this and doing this and practicing for it keeps me adept at my job and safe every day," Huffman said. "That's the main reason we come out here."
Young advanced to the Western Chapter Tree Climbing Championship, which is held in Santa Barbara, California in late March.


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Happy Monday to you!! I spent my weekend playing around in my craft room. I wanted a card that would be easy for first time stampers and I think this card fits the bill. I used the flower in Button Buddies and stamped it in Real Red and Pumpkin Pie (I saw this combination on the site of another stamper but I cannot recall her name). I threaded white Baker's Twine through a button from the Brights collection and adhered it with several glue dots. My sentiment is from Kindness Matters and I used Chocolate Chip ink. A quick but cute card!
Have a wonderful day!


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The 2014 Sustainable Brands conference in San Diego prompted discussions about a host of sustainability issues, from climate resilience to consumer engagement. But in a nation where waste recovery rates have hovered below 35 percent for the past decade, it was tough not to talk about recycling as well.
In a panel discussion hosted by the Cradle to Cradle Products Innovation Institute, leaders in the textile recycling space spoke about lessons learned that can be applied to the apparel industry, which is a key area of focus for the institute. One of the more surprising additions to the panel, entitled "Optimizing Building Blocks: Cradle to Cradle Materials for Textiles," was Paul Murray, vice president of sustainability and environmental affairs at Shaw Industries, the world's largest carpet manufacturer.
Believe it or not, there's actually a great deal the apparel industry can learn from carpet-makers about closed loop recycling. Shaw, for example, has been producing Cradle to Cradle certified products for more than a decade, and they now make up 64 percent of the company's sales. I sat down with Murray after the panel to find out more about how the carpet industry's success with closed loop recycling can be applied to your favorite fashions and lessons that translate across the textile industry.
1. Design with the end use in mind
The most obvious aspects of closed loop recycling happen in the collection and re-processing phases, but Murray noted that the process starts much earlier -- in the design phase. When recovering textiles for recycling, it's vitally important that the material components are nontoxic, safe for recycling workers and safe for the end consumer.
"It was early that we looked at [closed loop] as a system to prevent bad chemistry," Shaw said, noting that the materials assessment for Cradle to Cradle certification is much tougher than EPA standards. "Federal law makes us disclose everything up to 1 percent; Cradle to Cradle makes you look at that chemistry down to 100 parts per million, or one thousandth of a percent. So, it's 1,000 times stronger than the EPA would require of an apparel industry, but it makes things a lot safer for the general public."
Materials assessments look at everything from dyes and glues to the fabric itself. Beyond ensuring products are nontoxic, nuts-and-bolts design decisions, such as the construction of the product, also play a key role in making sure the product can be re-purposed at end-of-life.
"We want to make sure that those materials have a second life," Murray said of Shaw's carpeting. "So, we actually try to then manufacture the products so that they can come apart in one shape or form or another.
"The apparel industry has several issues that we have to deal with in a different way. One is how do you take a piece of, let's say, jeans and get the rivets and the zipper out so that the fabric could actually be used."
2. Take a branding approach to closed loop
Achieving Cradle to Cradle certification for a product is no easy feat, but that's only the beginning, Murray explained. To make a closed loop product successful, he said, companies need to make their customers aware of its value. To achieve this, he suggested a branding approach with which most companies are already familiar.
"Cradle to cradle is a concept, a process and a label," Murray said. "If nobody knows about that label, then you don't have brand recognition. People won't look for [the Cradle to Cradle certified label] on a piece of carpet if they don't know about it. So that's what I would call 'brand recognition' for the certification itself.
"As the apparel industry looks to cradle to cradle as a solution, they need to be thinking about what they [can] do to help that brand recognition," he continued. "How do we get to that partnership of building the Cradle to Cradle brand? I think it's important for all cradle to cradle companies to work together for that branding."
A select few apparel companies, such as Puma, have already started to build branding around their Cradle to Cradle certified products, while others, like Levi Strauss & Co., focus on branding around recycling through their own processes. But most would agree there's still much more to be done in this area.
3. Never stop looking for new technologies
When we spoke with global, end‐to‐end textile solutions provider I:CO last month, Jennifer Gilbert, the company's chief marketing officer, likened fabric recycling to paper in the sense that fibers shorten and degrade when they are recycled. Although the company has managed to devise end uses for several textile categories, Gilbert noted that the degradation of fabric fibers poses significant challenges, and she's right -- at least for now.
With most processes used today, recycled textiles must be combined with virgin feedstocks to compensate for fiber shortening, but cutting-edge techniques are under development across the country, Murray noted. Shaw, for example, uses a high-tech depolymerization process to turn grimy used carpeting into a brand new product. Since fibers are broken down at the polymer level, the result is essentially new fiber that is just as strong -- if not stronger -- than the original tossed carpeting.
"I think the technology will really change over time," Murray predicted, pointing to a fledgling effort in Seattle that devised a process to grind cotton so fine it can be extruded as new fiber -- much like the process used to make rayon back in the 1980s.
"There's going to be a lot of different developments in all sectors, whether it be carpet or apparel," he continued. "Even the carpet industry today: When you look at a piece of carpet it's glued together. The methodology to take that apart, we continually work on. So, that's an example of an industry where we have to work collaboratively to come up with methods to take things back."
Staying open to new technologies and supporting them financially when possible is key to moving the closed loop process forward, Murray said. Shaw, for example, partners with roughly 50 entrepreneurial recyclers across the country to recover its carpeting, while I:CO works with literally hundreds of partners from around the world to develop new recovery processes.
4. Be patient with infrastructure
"Everybody that talks about cradle to cradle makes this point: It really has to start somewhere," Murray said.
It's true that the infrastructure for textile recovery is growing, and companies like I:CO are a big part of that, but it can be easy for brands to become frustrated with the lack of take-back options or end uses for their products. This frustration, however, is no reason for companies not to pursue closed loop options and think about them in design.
"You really don't start with the infrastructure to take things back," Murray said. "You start with making sure that what you're producing is as safe and environmentally friendly as possible ... That's really where the apparel industry has to get strong."
As methods evolve for producing products that are safer to recycle and reuse, as well as for the re-processing itself, the infrastructure will slowly start to catch up -- especially if consumers are engaged and asking for closed loop products, Murray said.
To engage its own consumers, Shaw recently sent out the below video to its online mailing list, which details how Shaw uses closed loop processes and efficiently sums up the five basic tenants of Closed Loop certification.
Image credit: Flickr/bluehillranch
Mary has reported on sustainability and social impact for over a decade and now serves as executive editor of TriplePundit. She is also the general manager of TriplePundit's Brand Studio, which has worked with dozens of organizations on sustainability storytelling, and VP of content for TriplePundit's parent company 3BL.


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Flint airport stabbing suspect allegedly tried to buy gun in US but failed
An airport police officer was stabbed in the neck in the Wednesday attack.
— -- The Canadian man accused of stabbing an airport police officer outside the Transportation Security Administration screening area at a Michigan airport Wednesday had allegedly tried to buy a gun in the United States but failed, David Gelios of the FBI said.
The suspect, Amor Ftouhi, who is now in custody, legally entered the United States in Lake Champlain, New York, June 16, and later traveled to Flint, Gelios said. He was in Michigan as early as June 18, Gelios said today.
Gelios did not specify where Ftouhi allegedly unsuccessfully tried to purchase a gun. The knife was purchased in the United States along his trip from New York to Michigan, officials said.
Gelios today urged anyone who may have encountered Ftouhi while he was in Michigan or anywhere on route from New York to Michigan to contact authorities.
There is no indication that the suspect had any association with anyone in the Flint area, Gelios added.
Here is a closer look at how the alleged assault, which is being investigated as what officials called an "act of terrorism," unfolded, according to the complaint filed against the suspect, which cited surveillance video:
8:52 a.m.: Attacker arrives at the airport
Around this time Ftouhi entered the first level of the Flint, Michigan, Bishop International Airport carrying a duffel and a satchel bag.
9:10 a.m.: Suspect reaches the second floor
Ftouhi took the escalator to the second level of the airport. At the top, he turned left to go to a restaurant.
At about 9:37 a.m., Ftouhi left the restaurant carrying both bags.
At 9:38 a.m. he went into a second floor bathroom, and about 28 seconds later, he left the bathroom without either of his bags.
9:38:48 a.m.: The assault
After leaving the bathroom, Ftouhi allegedly attacked airport police officer Lt. Jeff Neville with a roughly 12-inch knife that had an 8-inch serrated blade, stabbing him in the neck.
Ftouhi allegedly yelled "Allahu Akbar" during the attack, the FBI's Gelios said Wednesday.
Gelios said the attacker "continued to exclaim 'Allah' and he made a statement to the effect of 'You killed people in Syria, Iraq and Afghanistan' and 'We're all going to die.'"
Daniel Lemisch, acting U.S. attorney for the Eastern District of Michigan, said today that officer Neville was assisted by citizens, airport workers and police officers, adding that he himself fought back against attacker.
Officials said that "Neville never stopped fighting" until the suspect was in handcuffs, which took about a minute.
The aftermath
The Wednesday morning attack halted travel and forced passengers to evacuate. The airport later reopened and all passengers were safe, officials said.
Neville was hospitalized and underwent surgery but is expected to fully recover. He was listed in stable condition Wednesday.
Ftouhi was taken into custody and a complaint was filed against him for violence at an international airport, but officials noted that there could be more charges in the future. The ongoing investigation is part of a joint operation with Canada, officials said.
The incident is being investigated as an act of terrorism. Officials said the investigation continues to see whether he committed other crimes, including a federal terrorism offense.
Officials said it appears the suspect "has a hatred for the United States and a variety of other things that motivated him to coming to the airport today to conduct this act of violence."
He was not on the radar of U.S. or Canadian authorities, officials said.
No one else appears to have been involved and there is no information to suggest a wider plot, officials said.
Ftouhi made an initial appearance in court Wednesday evening and he was ordered temporarily detained without bond, Lemisch said today. His next hearing is set for June 28. ABC News has reached out to his public defender for comment.


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Paperweights come in all sorts of shapes and sizes, different materials and designs. Murano for instance, is very famous for its glass paperweights and some can be very highly prized indeed. Another group come from Royal Crown Derby in England. In 1981 Royal Crown Derby issued a set of 6 animal paperweights filled with sand. They were the rabbit, owl, duck, quail, penguin and wren. Many more designs followed , all animal inspired shapes such as the Koala , Barn Owl, and Garden Snail.
They came with Gold or Silver stoppers to indicate the quality of the paperweight, Gold being a First quality example and Silver a second. The design and production quality of these is of the highest order.
There have been several changes over the years. Firstly the sand was removed to lighten them for shipping costs, especially to the USA. For a very short while a ceramic stopper was used, but customer feedback forced the gold and silver stoppers to come back, so having a ceramic stopper does not mean that you do not have a First Quality piece.
These paperweights became so desirable that in 1984 Royal Crown Derby created a collectors guild and members could get specially produced items that the general public could. Limited editions came along with runs of as little as 250 or less. An example of the guild designs was the "Debonair Bear" introduced in 1998.
What is really nice is the absolute variety of these designs. There are hundreds to choose from. Mostly done in the Imari, my personal favorite style, they produced this in different sizes and price ranges. Many of the earlier designs have been retired. Today, some can be obtained for as little as $35 to $50 without a box and certificate, and can go up to well over $2500 depending on the rarity. Having the original box and certificate always help the value. I have quite a few of these out on the shelf and really enjoy looking at them and have several more in our shop. Come check them out. They are still producing them today and are as popular as ever.
If you have a favorite, post a picture, we would love to see them.


text: We'll soon live in an age when netbooks have both CPUs and GPUs and it will be grand. Unicorns and rainbows will dance off of our screens in harmony — and Internet Explorer might become relevant again. The video after the jump shows what IE9 is capable of when a next-generation NVIDIA Ion GPU is paired with an Intel Atom CPU verses a standard netbook with an Atom all by itself. The differences are obvious and makes a solid case for Internet Explorer. But most of us will probably visit the International Space Station before setting IE as our default browser even if it renders graphics faster. [via liliputing]


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Autodesk VRED Presenter for mac 2020 displays the datasets created in VRED in high quality. The viewer can use the whole coverage of the defined navigation possibilities. All VRED specific elements of the surface have bee withdrawn completely and the steering of defined functionalities within the scenery can be carried out by HTML and/or flash applications.
VRED Presenter can only be used to look at images. Further work with the datasets is only possible with Autodesk VRED Design or Autodesk VRED Professional.
Autodesk VRED Presenter Mac Features:
- Multiple webengines can now be selected in the media editor tree with ctrl-left mouse.
- This feature evaluates transparency for interaction, allowing click through of geometry containing transparent media.
- Preferences have been added for annotation properties.
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- The visible state of the node that the annotation is associated with is used for the visibility of the annotation.
- When merging a vpb file that contains annotations, you can now select Import Annotations from the Merge Project Options dialog.
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- Showcase files are not supported in VRED and beyond.
- You can open a Showcase file in a version of VRED before, save the file (vpb), and open in VRED.
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- Multilayer EXR files can now be imported with meta data included.
- Luminance EXR files can also be imported and exported.
- You can now copy and paste between instances of VRED.
- With two or more sessions of VRED open, copy nodes from one scenegraph and select a node in another scenegraph and paste.
- Mac OS X Kodiak, 10.0 (Cheetah), 10.1 (Puma), 10.2 (Jaguar), 10.3 (Panther), 10.4 (Tiger), 10.5 (Leopard), 10.6 (Snow Leopard), 10.7 (Lion)
- OS X 10.8 (Mountain Lion), 10.9 (Mavericks), 10.10 (Yosemite), 10.11 (El Capitan) and
- macOS 10.12 (Sierra), 10.13 (High Sierra), 10.14 (Mojave), 10.15 (Catalina) and Later Version.
- Supported hardware: Intel or PowerPC Mac.


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This scotch glass was constructed from Pink color tubing and features silver fumed accents by in house PRISM artist Phil Sundling. It measures 3.5'' +/- tall, with a 2.75'' +/- wide base and 2.75'' +/- wide mouth.
(Glass pictured on black background represents what item will look like when filled with your favorite dark colored beverage! )
- 3.5'' +/- tall
- Holds 1.25 cups / 10 oz
- Pink
- Color tubing
- Silver fuming
- Made in house by Phil Sundling
Available on site in the PRISM Glassworks gallery in Troy, NY.