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Haynes:Mamalian IFC
Immunocytology of Fixed Cells
Karmella Haynes 2013
Culture Plates
This procedure allows you to fix and stain cells in a 24-well culture plate without mounting slides. Image quality and magnification is limited (10x - 20x), but processing larger sample numbers is easier.
- Adherent cells cultured in a 24-well plate
- Sterile 1xPBS (keep in the TC room)
- Paraformaldehyde 97% (dry) - Alfa Aesar A11313-22
- 20 mL aliquot of non-sterile 1xPBS in a 50 mL conical; for Fixing Buffer
- 10M NaOH
- 50 mL aliquot of non-sterile 1xPBS for washes (keep at your bench)
- Permeabilization Solution - Cold methanol or 0.5% Triton-X100 in PBS
- Note: Different antibodies work better with different permeabilization. Try both and see which works best for your specific antibody.
- Blocking Solution - 5% Normal Horse Serum in PBS
- Make Fixing Buffer: Work under the fume hood. Add 1.0 g dry 97% Paraformaldehyde to the 20 mL aliquot of non-sterile 1xPBS in a 50 mL conical tube. Add 25 μL 10M NaOH. Cap the tube, seal with parafilm, and heat at 60°C with occasional shaking until the paraformaldehyde dissolves. Cool the solution to room temperature (or chill in the refrigerator) before use.
- Do the following in the TC room in the biosafety cabinet: Remove medium from the cells. Add 0.5 mL warm Sterile 1x PBS to each well.
- Take the plate to your bench.
- Remove the 1xPBS from the cells.
- Work under the fume hood: Add 0.5 mL freshly made Fixing Buffer to each well. Incubate at Room Tmp./ 10 minutes.
- Remove the Fixing Buffer. Add 0.5 mL Permeabilization Solution to each well and incubate at room temp. for 10 min.
- Remove Permeabilization Solution and rinse cells with 0.5 mL non-sterile 1xPBS for washes.
Note: This is a stopping point. You can place a lid on the plate, seal it with parafilm, and store the cells +1xPBS at 4°C for several days. Or, proceed immediately to Antibody Staining.
Antibody Staining
- Block in 250 μL Blocking Solution for at least one hour at Rm. Tmp. (can incubate overnight at 4°C if necessary).
- Dilute primary antibody (according to supplier's instructions) in Blocking Solution'. Add 15 μl on top of the cells and cover with a 1 cm2 piece of parafilm. Incubate at room temp. for at least one hour in a humidified box (incubate overnight at 4°C if necessary).
- Wash 3 – 4 times/ 5 min. with non-sterile 1xPBS.
- Dilute secondary antibody and Hoechst (DNA stain) in Blocking Solution (see table).
- Add diluted secondary to samples (same way as primary) and incubate at Room Tmp. for one hour. Protect the samples from light; fluorophores are light-sensitive.
- Wash 3 – 4 times/ 5 min. with PBS.
Mounted Slides
This procedure is for mounting slides. The process is more involved, but image quality is greater and cells can be imaged under higher magnification (40x oil objective)
REAGENTS: In addition to the reagents listed for Culture Plates...
- PLL Solution - 0.01% Poly-L-lysine solution (Sigma-Aldrich P4707)
- Mounting Medium - Polyvinyl alcohol mounting medium with NPG (Sigma-Aldrich 10979)
Cell Culturing on Glass Cover Slips
Perform all of the following steps in a biosafety cabinet in the TC room.
- Place a single sterile round cover slip into each well in a 24-well dish.
- Add 200 uL polylysine solution to each well. Incubate at room tempertaure for 5 min.
- Remove the polylysine solution from each well. Let the slips dry in the biosafety cabinet, uncovered for at least 30 min.
- Seed cells at a fairly sparse density (non confluent). Allow the cells to attach to the cover slips (~ 8 hours)
- Follow instructions for Culture Plates.
Antibody Staining
- Follow instructions for Culture Plates.
Mounting and Storage
- Place a small drop of Mounting Medium onto a clean rectangular glass slide.
- Invert a cover slip, cell side down, onto the medium and press gently.
- Blot excess mounting medium with a Kimwipe (be careful not to slide the cover slip out of place).
- Rinse briefly with distilled water and blot dry.
- Slides are best viewed after allowing the mounting medium to set overnight (at Room Tmp., protected from light); slides may be viewed immediately if handled carefully.
- Slides may be stored indefinitely at room temp. (morphology is preserved)
Dilution Guide
Dilutions of some commonly used secondary antibodies and dyes.
|Secondary antibody conjugate or dye
|Visible color
|green (FITC)
|Alexafluor 488
|green (FITC)
|Hoechst 33325


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Story highlights
- "I didn't have any boyfriends, but lots of crushes," Charlize Theron says
- "I wasn't in the popular crowd," she says
- She also says she was bullied by her classmates for her glasses
Playing a nasty schoolgirl in the new dark comedy "Young Adult," Charlize Theron recalls her own high school days as the girl who never fit in, especially with the boys.
"I wore really nerdy glasses because I was blind as could be and the boys didn't like [me]," the actress, 36, told PEOPLE recently in New York. "I didn't have any boyfriends, but lots of crushes."
Ignored by a boy she had a crush on, she says she had longed to be part of the in-crowd, causing her to do "crazy things."
"I wasn't in the popular crowd. There was a really popular girl at school and I was obsessed with her. I mean you would go to jail for that stuff today," Theron says with a laugh. "I was in tears one day because I couldn't sit next to her."
She also says she was bullied by her classmates for her glasses and not having the perfect haircut and clothes.
"I actually got a lot of the mean girl stuff from the ages of 7 to 12. I was pretty much a mess in primary school," she admits. "But I got that out of my system by the time I got to high school and was more immune to all of that stuff."
"Young Adult" opens December 9.

subset: commoncrawl


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Bats look to get back on track at Fenway
With just eight hits in two games, club looks for home cooking
By T.R. Sullivan / MLB.com
A painful and historic lack of offense is crushing the Red Sox's chances of advancing to the American League Championship Series, and they have just one more game to figure this out. If they don't, their season is over.
"We need to execute and swing the bats better," third baseman Mike Lowell said after the Red Sox lost to the Angels, 4-1, in Game 2 of AL Division Series on Friday night.
The Red Sox are down, 2-0, going into Game 3 on Sunday, and all of New England awaits to see if their sluggers can start hitting at Fenway Park like they have all year.
"This is not the way we scripted it, but you're not eliminated until you lose that third game," Lowell said. "It will be a great feeling going back to Boston. We just need to focus on that third game on Sunday."
In the course of dropping the first two games, the Red Sox have made some club postseason history. They have just one run on eight hits in two games. In 151 playoff games dating back to the 1903 World Series, the club has never been limited to just one run or as few as eight combined hits over two consecutive playoff games.
They've run across many great pitchers in their postseason history, from Roger Clemens, Dwight Gooden and Dave Stewart, to Hall of Famers Bob Gibson, Pete Alexander and Christy Mathewson, but they have never had this tough of a time scoring runs or getting hits over a two-game stretch.
"It happens," second baseman Dustin Pedroia said. "It's two games, but it's more magnified in the playoffs. We have to swing the bats and we have to have better at-bats. We need to win. No matter how you do it, we need to win."
The Red Sox scored just one run on three hits in a 3-1 loss to Tampa Bay in Game 7 of the ALCS in 2008. So it's actually two runs on 11 hits in their past three playoff games.
The Red Sox are hitting .131 for this series. The lowest batting average by a team in a Division Series is held by the Texas Rangers, who hit .141 in scoring one run over three games against the Yankees in 1998.
"We're just not getting hits or getting men on base," Kevin Youkilis said. "I've been one of the biggest culprits. We've got to step it up in Game 3."
The Red Sox return to Fenway Park. That should help. The Red Sox scored an AL-leading 481 runs in 81 games at home this year. Their .498 slugging percentage at home was also the highest in the league.
"It's not comforting going down 0-2, but that's the position we are in," left fielder Jason Bay said. "The silver lining is we're going home. Hopefully we can force another game back out here."
Designated hitter David Ortiz seems to have struggled the most. He is 0-for-8 with four strikeouts in two games. He is hardly alone.
Lowell is 0-for-7, including grounding into one double play. Bay and J.D. Drew are 1-for-5, Pedroia is 1-for-8 and Youkilis is also 1-for-8, with a double that stands as one of two extra-base hits in two games.
Jacoby Ellsbury has been Ted Williams compared to his other teammates. He is the only Red Sox player with more than one hit, going 2-for-7. He had a triple in the fourth inning on Friday and scored on a single by Victor Martinez.
That's Martinez's only hit in seven at-bats and it's the Red Sox's only hit in nine at-bats with runners in scoring position over two games.
"It's not like we are squandering scoring opportunities," Bay said. "It would be one thing if we were getting guys on base every inning and not getting them home. We're not getting many scoring opportunities at all, and that's because of their pitching.
"We're a good offense and we have been all year. But people tend to forget those two guys out there are not just flipping the ball up there."
T.R. Sullivan is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.


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Drunk Driver Douses Self in Human Poop to Throw Cops Off His Scent
After he plowed his Jeep into a condo's garage by mistakenly flooring it in reverse, Gordon Flavia, a 57-year-old man from Longview, Washington, found that he'd driven clear into a makeshift bathroom containing a portable toilet. He then "doused himself with a bucket of liquid human waste" and hid inside the Porta Potty. Police found him there, soaking wet.
"We didn't know exactly what it was ... but it smelled bad," Police Sgt. Doug Kazensky said.
Flavia told officers he'd splashed himself with the contents of a bucket outside a portable toilet "because he thought the dogs were coming, and he was trying to throw off the scent," Kazensky said.
Added Longview police Sgt. Ed Jones:
"It looks like alcohol was involved."
Previously: Peeping Tom Hid Inside Porta Potty [TDN via Fark, photo via Shutterstock]


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Wooded Wonderland
Welcome to Galena & Jo Daviess County, IL
Embark on a journey back to a time where working hands crafted and molded the essentials of life. Hand-made tables and benches, willow chairs and swings, keep-sake boxes and wine holders, plant stands and chests, all crafted by local artists from local timber.
Wooded Wonderland, in Galena, IL, offers a unique opportunity to explore age-old craftmanship at its finest, in a variety of trades. A working forestry program and sawmill supply the material for all our projects. Group and individual tours are available.
Explore the unique tresures in the Country Store, taste the richness of honey claimed from the sweetest blossoms and delight in the aroma of nature, pure and simple.
Organically grown high-bush blueberries are available for picking July to September.
Please call for berry conditions. 815.777.1223
Come visit Galena and enjoy!
© 2018 Wooded Wonderland, Galena, Illinois
Designed by Captivate Inc. Galena, Illinois


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This resource indexes journals covering cultural, economic, political & social change. Coverage is international and interdisciplinary. Indexes nearly 300 alternative, radical and left periodicals, newspapers and magazines. The theories and practices of socialism and revolution which this database covers inlcude: Ecology; Democracy and Anarchism; Feminism and Organized Labor; Indigenous Peoples; and Gays & Lesbians.
Resource URL: http://search.ebscohost.com/login.aspx?authtype=ip,uid&profile=ehos...
Online Help: Click "?" button
This resource is funded by:


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Suns finish behind Ark. State, Ole Miss in Tech Twilight Run
By Sean Ingram Sports Editor
Arkansas Tech University's cross country Golden Suns seem to be stuck on third early in the 2010 season, but that's OK. When NCAA Division I schools Arkansas State and Ole Miss finish ahead of you, that's not too shabby.
The Suns, also ranked third in the NCAA D-II South Region, finished third out of six teams Friday night during the fourth annual Tech Twilight Run, a 2-mile race on the grounds surrounding Tucker Coliseum. They were led by freshman Cheryl Nolan of Carlow, Ireland, who finished fourth overall in a time of 11:49.65.
Arkansas Tech, which has finished fifth, second and fourth in the last three Twilight Runs, had runners finish in the top 10. Sophomore Bailee Miller of Dover, the reigning Gulf South Conference freshman of the year, came in ninth overall with a time of 11:56.56.
The Golden Suns finished with 61 points, 15 behind second-place University of Mississippi (46) and meet champ Arkansas State (37), which had four runners in the top 13. Three-time defending Tech Twilight champ Arkansas-Little Rock finished fourth (79), followed by University of the Ozarks (152) and Hendrix College, which had no team score and had only four runners compete.
"I was pleased to have four of five runners within 20 seconds of one another, but we need to get all five of them at that same level," Tech cross country coach Tom Aspel said. "I'm also pleased we had everyone (finish) under 14 (minutes). You'd like to win your home meet, but we need a fifth runner to break through.
"It was a tough race, and I thought we competed well. It was a good way to open the season."
Golden Suns senior Michelle Pollreis of Gravette finished 15th overall (12:08.41), followed by freshman Kaitlin Bounds of Russellville (12:09.29) in 16th place. The final three finishers for Tech were senior Lauren Karnitz of DeSoto, Kan. (13:30.88, 33rd), freshman Ashley Perisho of West Plains, Mo. (13:43.22, 37th) and freshman Amanda Bradbury of Bauxite (39th, 14:01.45).
ASU's Kristina Aubert won the race with a time of 11:18.41. Logan Waites of Ole Miss finished second (11:25.44).
UALR won the men's race for the fourth consecutive year (26 points), followed by Ole Miss (41) in second and Arkansas State (87) in third. Hendrix and University of the Ozarks (125) tied for fourth, and Henderson State (147) finished sixth overall. Ole Miss' Barnabas Kirui won the men's 4-mile race with a time of 19:39.77. UALR's Johnny Purvis was second (20:05.17) for the second straight year.
The Golden Suns travel to Little Rock on Friday for the UALR Invitational at 6 p.m. at Rebsamen Golf Course.
University of the Ozarks
Lady Eagles results
36. Samantha Sparks, 13:41.89
40. Tadera Garland, 14:38.37
44. Lindsey Kellaway, 18:15.74
45. Susan Rummel, 23:29.04
46. Cassie Saorrono, 23:45.58


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Rockies down 2-0 after narrow defeat
Red Sox do just enough to beat effective but wild Jimenez
By Thomas Harding / MLB.com
But all the winning in the interim no longer matters, because these two games have been in the World Series. Thursday night's 2-1 loss to the Red Sox in Game 2 at Fenway Park before 37,730 puts the Rockies' October dreams in jeopardy.
The Fall Classic picks up at Coors Field on Saturday night for the first home World Series game in Rockies history. It will be the beginning of a tall task. Of the 50 previous teams that have dropped the first two games, just 11 have come back to win titles -- the last being the Yankees in 1996.
Of course, the Rockies, who are also home on Sunday and, if necessary, Monday, can lean on having won 13 of their final 14 regular-season games, including a one-game National League Wild Card tiebreaker with the Padres just to qualify for the postseason.
"I think it's very comparable," first baseman Todd Helton said. "It's not like we have to win every game, but darn near.
"We've lost two games in a row before. It's been a while, no doubt about that. But it's baseball. Offense gets hot, it gets cold. ... You look at a seven-game series as a mini-season and just turn it around."
The Rockies gave a much better account in Game 2 against the Red Sox than they did while taking a 13-1 beating in Game 1.
Starter Ubaldo Jimenez was wild, walking five while giving up two runs in 4 2/3 innings. In the second inning, Mike Lowell walked, took third on J.D. Drew's single to right and scored on Jason Varitek's sacrifice fly. After retiring two straight in the fifth, Jimenez walked David Ortiz, then yielded a Manny Ramirez single and a Lowell RBI double for the winning run.
"I thought for a first-year player up here, pitching in this venue against this ballclub, he gave us everything he had," Rockies manager Clint Hurdle said.
Rockies relievers Jeremy Affeldt, Matt Herges, Brian Fuentes and Manny Corpas held the Red Sox to three hits and two walks in 4 1/3 scoreless innings.
But the Rockies will need an about-face offensively.
The Rockies went 1-for-13 with runners in scoring position, scoring their only run in the first on a Helton fielder's-choice grounder against Curt Schilling.
But Schilling frustrated the Rockies when they put runners on base. The veteran lasted 5 1/3 innings before giving way to a lights-out bullpen performance by Hideki Okajima (2 1/3 innings, four strikeouts) and Jonathan Papelbon (1 1/3 innings, two strikeouts, a key pickoff and the save).
The Rockies also fanned 10 times to bring their Fall Classic total to 22, and they have a .180 batting average through two contests.
Colorado entered with confidence from having outscored the Boston, 20-5, in a June Interleague Play series at Fenway Park, while taking two of three. But in dropping the first two of the biggest series in the world, they've managed just two runs.
The Rockies became the second team in history to win their first seven postseason games, but it wasn't until the Series that their offensive woes have hurt them. They have a .229 cumulative postseason batting average.
That includes leadoff man Willy Taveras (0-for-3, hit by a pitch Thursday, .120 postseason overall), Garrett Atkins (0-for-4 Thursday, one RBI and a .171 postseason average) and Brad Hawpe (six strikeouts in eight at-bats in the World Series). All were key contributors to the Rockies making the playoffs.
Matt Holliday had four singles, but none drove in a run. Holliday singled off Papelbon in the eighth with two outs, only to be picked off with Helton at the plate to end the inning.
"I don't know if there's any explanation for it, just a few guys struggling right now," Atkins said.
"That makes it tough to win, and the guys on the bubble-gum cards are pretty good, too," Hurdle said. "They had some pitches to hit tonight, didn't square it up, and Schilling got us. ... He's a competitor."
Schilling, 40, no longer has an explosive fastball. He has used guile this postseason to quell teams after they put runners on base. Either way, he is now 11-2 in the postseason for his career.
Schilling hit Taveras in the hand to begin the game. Holliday singled off the glove of Boston third baseman Lowell, and the Rockies wound up with runners at second and third when Lowell threw wildly trying to nab Taveras. But Helton grounded out for what turned out to be the only run of the night for the Rockies, and the rally ended with Atkins' grounder to third.
On Saturday, the Rockies will trust the loudness of a sold-out Coors Field and the savoir faire of righty Josh Fogg, who will face Red Sox righty Diasuke Matsuzaka. Teammates call Fogg "The Dragon Slayer" for his penchant for outdueling big-name opposing pitchers.
Matsuzaka is a rookie sensation more than a big name, but because of the Rockies' plight, they're stepping into the fire no matter who is opposite Fogg on the mound.
"Two games," Hawpe said. "We need to win one more than they do. We're going home. We'll go back there and start over fresh."
Thomas Harding is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.


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But how do you do that when you have a monkey on your back?
Between children, co-workers, construction, and other "c" words that stand for chaos, it is a wonder we can get anything written at all. This is when you need to develop some habits that create calm and order in the middle of mayhem.
Here are some tips on writing and staying productive—even when confusion is knocking on your door:
1. Pick an off time to write.
It might be early in the morning, late in the afternoon, or even in the middle of the night. You know when it is in your life: That time when everything seems to settle down for a little while, or right before it starts all over again. If you can find this prime time and get writing, you will be a lot more productive.
This might mean that you get up before everyone else, but so be it. If early morning is not your thing, try for mid-afternoon. While everyone else is munching on chips and staring at the clock to see how many minutes are left until it is time to go home, be productive and write. If you are a night owl or have flexible hours, the witching hour is a great time to get a lot of writing done in peace and quiet.
I would not suggest writing when the kids get home from school, your spouse gets home from work, or during meal times. It may be just a wee bit distracting.
2. Set aside time to write every day.
Beyond finding opportune times to write, you also need to make writing a habit. When you make writing a part of your day like any other necessity (eating, sleeping, etc.), it can make it feel like less of a struggle. Habits are easy to form and hard to break, so make it a habit to write.
When you set aside a certain time every day to write, you also get less crud from those around you. If you are always busy at 4 p.m., chances are that nosy co-worker will stop bothering you after a week or two.
The same goes for working at home. If the neighbor wants to chat every afternoon but you are busy elsewhere, she will eventually get the picture.
Setting aside a time to write means fewer interruptions and more work accomplished. Setting aside the same time every day also means your brain will be ready to cooperate when you sit down. It will be used to gearing up for writing around that time, and just like when you get hungry around noon, you will start to get antsy to write around your accustomed time.
What a great natural motivator, right?
3. Make a space.
When it comes to chaos, a closed door is your friend. Pick a space to write where you can get away from it all. The living room or kitchen isn't a good place, nor is anywhere close to the water cooler or coffee pot. Places where others hang out do not make good writing spaces. Instead, try to find a quiet corner or room with a door you can shut, and tune out the rest of the world.
You should also set up an environment that promotes writing. A solid desk, an ergonomic chair, and other basic necessities are a good idea. Do not try to use the edge of your bed, or balance your laptop on a windowsill. It is not pretty, and not conducive to writing long term.
To be a productive writer, you also need to be organized. That means keeping your office space your own. I know it is not always possible, but do your best to keep your stuff in your hands only.
4. Crank up the tunes.
If noise is your problem, I recommend investing in a good headset or ear buds. Crank up the music and get writing. For people like me, music with lyrics is out. I have a tendency to listen to the lyrics instead of concentrating on what I'm writing. For those like me, I recommend instrumental music or nature sounds, like ocean waves or rain.
If you are stressed and need to think, turn on calm music. But if you want to write fast, crank up something a little quicker. Remember, the faster the beat, the faster you type.
5. Turn off the phone.
There is almost no situation where you can't turn off your phone for an hour or two. Alert the masses with a text and then shut it off. No one will pine about not being in contact with you for a short time.
Do you remember the time before cell phones? Harken back to a quieter, more independent time and shut it off.
6. Get anti-social.
If social media is your time waster of choice, shut it down. You do not have to check Facebook, Twitter and Pinterest every five minutes. You won't miss anything.
I have found myself losing hours of valuable writing time on social media before. Instead, use it as a reward. If you write two pages, you can look at three tweets, and so on. After a while you will find you don't miss it.
7. Learn what an emergency is.
There are emergencies, and then there are emergencies. Some things can wait and others can't. Learn to tell the difference and prioritize.
Giving the dog a bath can wait until after writing time. Taking your child to the hospital can't. Make writing a priority and think before you act.
Remember, it's OK to tell people "no" or "later."
8. Take notes.
Often when I sit down to write, especially in a chaotic environment, I blank out completely. I can't think of any of the great ideas I had just days or even hours ago.
Instead of losing all those great thoughts to the chaos that is my life, I carry a pen and notebook with me wherever I go. That way I can catch inspiration when it strikes!
Don't think a word or two will suffice, either. I still have a paper with "blindfolded coconut" written on it, and I have no idea what that means; I just remember it was incredible. Instead of just jotting a word or two, take a few moments to write down the whole idea, outline and all if need be. Make a list of the important points and possible title ideas.
The best thoughts often happen at the most inopportune times, but don't let that stop you from capturing and saving them for great writing fodder later. You can also do this when unusual or interesting things happen in your life. Did you hear about a great site? Write it down. Did you see something cool? Write it down.
Just like a photographer always needs to carry a camera, writers always need to carry a notebook and write down ideas as they come up. It can save you a lot of frustration and give you some great ideas when you draw a blank.
These are just a few of the ways I've learned to write despite the junk going on around me. Most of our lives are not a calm sea—they are more like a hurricane. Sometimes you just have to do your best to hang on and keep going.
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Box of WWII ammo found in Bellows Falls basement
BELLOWS FALLS -- A collection of World War II munitions were recovered from a village residence on Monday and taken upstate by the Vermont State Police bomb squad to be examined by members of the Vermont Air National Guard.
Bellows Falls Police Detective Sgt. Shane M. Harris said the home is located at the north end of the village, off Minard Pond Road. On a tip from the homeowner, a cache of WWII ammo, including two grenades, high-explosive mortar rounds and anti-aircraft rounds, were taken, he said.
Capt. Ray Keefe, Troop "D" Commander of the VSP, said the bomb squad assessed all the munitions and believed they appeared to be demilitarized, though one grenade was questionable. He said they were brought to the Derby area to be analyzed.
Lt. Col. Lloyd Goodrow, Vermont Air National Guard public information officer, confirmed that the Explosive Ordnance Disposal team at the Guard was aware of the materiel but had not received them as of Wednesday evening.
"It was explained to me that the military has a 'cradle to grave' responsibility to care for all such recovered military explosives," he said in an e-mail. "They have not yet been delivered, but are expected."
Keefe said the homeowner is a woman whose father was a military veteran and had brought home some souvenirs. Keefe said the woman, whom he declined to identify, found the munitions with Japanese writing on them in a box in the basement.
Harris, who believes the munitions are nearly 70 years old, said the homeowner immediately took photographs of the ammo and brought it to the Bellows Falls Police Department shortly before 1 p.m. He said he then contacted the VSP.
"It was an awesome response," he said in a telephone interview. "You couldn't ask for a better organization."
A 250- to 300-foot perimeter was established around the home by police and nearby residents were asked to temporarily leave the area for their own safety. Harris said Sgt. Chris Brooks was at the scene and reported there was full cooperation by neighbors.
Keefe said the scene was cleared by 6:30 p.m.
He said everyone from the homeowner, who did the responsible thing by not touching the items, to the authorities did a great job handling the situation. He said the best thing to do when suspicious materials -- like any military munitions that are not known to be safe or legal -- are found is to leave them alone and contact the police.
"Leave it where it is. We'll come to you," he said.
Domenic Poli can be reached at email@example.com, or 802-254-2311, ext. 277.
If you'd like to leave a comment (or a tip or a question) about this story with the editors, please email us. We also welcome letters to the editor for publication; you can do that by filling out our letters form and submitting it to the newsroom.


text:
About This Organizer
Our Promise – Young people want to shape the world. Camp Fire provides the opportunity to find their spark, lift their voice, and discover who they are. In Camp Fire, it begins now. Light the fire within.
Camp Fire San Diego has been serving the county for over 75 years. Providing an array of year-round programs in the community, at Camp CaHiTo and at Camp WoLaHi that give youth the tools they need to be successful at home, at school, and in our larger community.
Organizer Info
Camp Fire San Diego


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Well?I'm gonna stay positive, but this might be out best chance at rebuilding.
Well?I'm gonna stay positive, but this might be out best chance at rebuilding.
The problem with that is that we only have maybe two players who would get us a first rounder (Wilson and Gallo)
And Walker and Douglas are probably only worth a second rounder....well maybe considering Walker's low salary a team would give up a late first that would be making the same amount as him.
But no one on our team would be worth a lottery pick, so I do like the idea in theory, but a bunch a future mid first rounders would only amount to the type of team we currently have!
Then again....if we were to sign and trade David Lee to a lottery team over the cap (ie. Utah for the pick of ours they have this year) that would be smart.
Jason Kappono Willie Green and their pick (likely the 6th) this year For Lee would work
Or TJ Ford Solomon Jones and the 10th pick for Lee would work to.
Who knows?
"I'd Rather Melo shoot with 3 men on him than Jefferies shotting wide open"
-Jeff Van Gundy
Man this draft is pretty deep with big man we can definitly get two good solid players in da 2nd round.... Donnie did talk about he might maybe buy a late 1st rounder
Every time Donnie's on the radio he specifically says he chose to go this route (getting under the cap) so we didn't have to wait years to build from draft picks.
Something will work. We don't go through what we did the last few years and back away now.
The Thunder -- a paradigm -- also got a 1x/decade luckout walking into Durant. And it still has been an acimulation of 4 top lotto picks, multiple years, and they are still a 1sy round bustout of playoffs.
And again, they received a true godsend in Durant.
Drafting is NOT the way to go or us (especially since we will be relinquishig the little talent we have for these picks)
Have faith. This OS is ripe and we are primed. I guess if the apocolyose hits we'd need to look to picks...even then:
I'd rather sit and wait but 1 yr for a new FA-or-bust-bonanaza if a apocolyose hits and sit on what talent we do have vs risking it on a deaft&pray course.
There's nothing wrong with drafting but the problem with us is that we have no draft pick and trading for a draft pick would require us to dump either Wilson Chandler or Danilo Gallinari.
I don't want to dump either of those players for a draft pick, especially since I think we can trade Wilson Chandler and Eddy Curry and cash to the Hornets for Okafor and Collison rather than trade Chandler for a draft pick


text:
International Equine Disease Report, Third Quarter 2012
The International Collating Center, Newmarket, United Kingdom, and other sources reported the following disease outbreaks in the third quarter of 2012".
Contagious equine metritis was reported from Germany. Infection with the causal bacteria, Taylorella equigenitalis, was confirmed in non-Thoroughbreds: two stallions and three mares on one premises and a stallion on a second premises.
One case of equine coital exanthema (equine herpesvirus-3) was diagnosed in Kentucky.
Strangles was recorded in Germany, Ireland, Switzerland, and the United States. Ten cases were reported from Ireland. Outbreaks involving 17 horses on two premises occurred in Switzerland. Single cases and outbreaks of strangles were reported from Maine, Kentucky, and Pennsylvania in the United States, where the disease is endemic.
Outbreaks of equine influenza were reported from Argentina, the U.K., and the United States. The disease was confirmed at three Thoroughbred racetracks in Buenos Aires; the virus was a strain of Florida, clade 1 sublineage. The U.K. diagnosed influenza on two premises. Several cases of influenza were confirmed in Kentucky and Wisconsin.
Equine herpesvirus (EHV)-related diseases were reported from Argentina, France, the U.K., and the United States. Argentina reported five cases of EHV-1 abortion (non-neuropathogenic strains) in 2 of 10 non-vaccinated Thoroughbreds and 3 of 70 vaccinated Thoroughbreds. In France, EHV-1 and 4 were implicated in a respiratory outbreak in Thoroughbreds at a training center; also reported was a single case of EHV-1 neurologic disease. The U.K. noted outbreaks of EHV-4 respiratory disease on 10 premises and one case of EHV-1 neurologic disease. EHV respiratory disease was also reported in the USA. Several cases of infection with EHV-2 or 5 were diagnosed in California, Kentucky, and Ohio.
Ireland reported cases of Pneumocystis carinii infection in a group of foals.
Equine infectious anemia was confirmed in Canada, France, Germany, and the U.K. Single cases were diagnosed on two premises in Alberta and one in the Yukon. France reported the infection in three Camargue horses. Germany reported several outbreaks: four in one state, two in another, and single premises in two other states. The index case on one premises was in a foal that received plasma therapy; the donor mare was positive. Upon investigation, six other plasma recipients were found to be EIA positive.
Equine piroplasmosis was reported from France (endemic), Spain (endemic), Switzerland, United Arab Emirates (endemic), and the United States. Switzerland confirmed one case based on clinical evidence and serologic findings. The United States reported testing more than 190,000 horses since November 2009; no new cases of infection were detected in the third quarter.
In the United States, the number of equine cases of Eastern equine encephalomyelitis during the third quarter was 35; the national total is 171 cases in the third quarter of 2012. The number of affected states went from 6 to 16 with the greatest increase in disease cases confirmed in Louisiana, Mississippi, North Carolina, and Alabama. The number of equine cases of West Nile encephalitis rose dramatically in the third quarter in the United States; a total of 427 cases were reported from 40 states, with maximal increases in Texas, Pennsylvania, California, Indiana, and Iowa.
Single cases of Hendra virus infection were confirmed on three premises in Queensland, Australia, all fatal.
Vesicular stomatitis infection (New Jersey strain) was diagnosed in 25 equine (on 18 premises) in New Mexico and Colorado.
Salmonellosis was reported by Ireland (three cases) and the United States. S. agona and Salmonella spp. belonging to Groups B, C1, and C2 were involved.
Argentina recorded limited outbreaks of rotavirus Group A infection in foals.
Equine monocytic ehrlichiosis was reported in the United States, with multiple cases diagnosed in Ohio and Kentucky.
An isolated case of Anaplasma phagocytophilum infection was recorded in Switzerland.
*Second Quarter Report for Australia
This is an excerpt from Equine Disease Quarterly, funded by underwriters at Lloyd's, London, brokers, and their Kentucky agents.
About the Author
Equine Disease Quarterly
Related Content
2013 EHV-1 Tracker Map
Equine Herpesvirus-1 Refresher
Equine Influenza
Veterinarians Urge Vaccination against West Nile Virus
Increased Equine West Nile Virus Activity in 2012
Complicated Equine Skin Diseases
EHV-1 Inactivated Vaccine Efficacy Tested (AAEP 2011)
Equine Infectious Disease Control in Developing Countries
Neurologic EHV-1: An Overview
Piroplasmosis: Cayenne Tick Ruled Out as T. equi Vector


text:
The City of Richardson will continue a modified version of Stage 3 water restriction measures through the month of May. The restrictions limit lawn irrigation to twice a month and prohibit watering between 10 a.m. and 6 p.m.
In past years the City has been able to alleviate restrictions during the spring and summer to allow once-per-week watering, but the North Texas Municipal Water District (NTMWD) has given direction to all of its member cities to continue only every-other-week watering due to critical water shortages in its water supply. NTMWD reservoir elevation numbers are significantly lower than this time last year. As of Wednesday, Lavon Lake's elevation was down 11.7 feet, and Chapman Lake was down 10.8 feet.
The schedule below is based on guidelines approved by the NTMWD:
The NTMWD, which supplies water to Richardson, has recommended that its member cities restrict watering to once every two weeks because of the ongoing drought and loss of Lake Texoma as a water source due to a zebra mussel infestation. A new pipeline that was built to address the issue should be operational in the coming weeks, but even the addition of that water will not be enough to relieve the concerns over currently low water reservoir levels. NTMWD officials are expected to review drought conditions and discuss future recommendations at a meeting on May 22.
Richardson residents are encouraged to help save water by turning off irrigation systems and watering lawns only when needed. Moisture meters are available from the City as part of the Outdoor Water Conservation Kit for $5.
Learn more about the water restrictions at cor.net/waterconservation or through the MyRichardson mobile app, which provides a schedule of specific watering days and the ability to report violations. Watch a City Council work session about the continuation of the modified Stage 3 water restrictions at bit.ly/1m7K0Wf.
411 W. Arapaho Rd. Richardson, TX 75080-4551 PH# 972 -744-4100 | Mailing Address P.O. Box 830309 Richardson, TX 75083-0309
© 2018 City of Richardson, TX | Privacy Policy
| Accommodations for Persons with Disabilities call, 972-744-0908 or Email firstname.lastname@example.com


text:
A PACE contact parliamentarian supports the ONE in FIVE campaign in Cyprus
The campaign has so far encouraged the citizens of Cyprus to sign a petition urging the government to adhere as soon as possible to the Council of Europe's Convention on the Protection of Children Against Sexual Exploitation and Sexual Abuse (Lanzarote Convention), which was signed on 25th October 2007 by the Republic of Cyprus.
The future plans for the campaign in Cyprus will be the translation of the awareness raising material The Underwear Rule into Greek in order to inform and empower the parents, carers, children, youth and public at large, and a contact series of lectures in the universities around Cyprus on sexual exploitation of children.
Read more about the campaign here


text:
There are lots of reasons to use clean, short, readable URIs. Search engines like them. People have some hope of dictating or typing them correctly. Email clients are less likely to mung or truncate them. They give people navigational cues and an extra way to navigate a website. You can even fit them on one billboard (unlike say this one).
One generally ignored advantage is security.
Many phishing, XSS, CSRF and all URI exploits rely at least in part in part on putting stuff the user does not understand in the URI.
Here are a few real URIs from popular websites all found inside a minute within 3 clicks of the home page:
- https://chat.bankofamerica.com/hc/LPBofA2/?visitor=&mse
- https://signin.ebay.com/ws/eBayISAPI.dll?SignIn&UsingSS
- https://www.amazon.com/gp/sign-in.html/103-1490903-7108
- https://www.google.com/accounts/ServiceLogin?service=ma
Having let people get used to that sort of garbage from sites that they should be able to trust, you can't really be surprised that normal people can't tell the difference between an XSS attack hidden in URL encoded JavaScript and a real, valid, safe URI. Even abnormal people who can decode a few common URL encodings in their heads are not really scrolling across the hidden nine tenths of the address bar to look at that lot.
It won't help everybody. There are always going to be people who are happy to believe that their bank sends them email from a free address like firstname.lastname@example.com, and sufficiently sophisticated social engineering is always going to work on some people, some of the time, but the sites that are particularly popular with phishing attacks are making it unnecessarily easy.
If commonly used sites had short, sensible URIs it would not take genius on the part of slightly cynical users to notice that every real bank URI they had seen in the past looked something like https://www.bankofamerica.com/myaccount/login so the 300 character monstrosity full of percent symbols and ampersands that they were being presented with is a little on the fishy side.
Now, go and tidy your room.


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Ziggy Palffy retired Wednesday—which means that Tuesday, Ziggy Palffy still was an active professional hockey player.
Palffy, 42, apparently got tired of dominating Slovakia's top league; he had 26 goals and 47 assists in 39 games last season, which was in line with his production since he left the NHL in 2006 for family reasons after 42 games with the Pittsburgh Penguins.
"One morning is more beautiful than the previous one, and you have other opinions," Palffy said, as per the IIHF website (via Pro Hockey Talk). "At this point, I believe that I quit. Definitive.
"Already I do not want to travel anywhere. Did it enough."
Indeed. In 684 games with the New York Islanders, LA Kings and Penguins, Palffy averaged more than a point per game and scored more than 40 goals three times. In short, he was one of the best wingers of his era—and maybe it's because his NHL career was over at 33, but that's gotten lost in the shuffle.
He's also one of a few Czechoslovakian-born 90s-era standouts who still are playing (and not named Jaromir Jagr or Miroslav Satan). That country split into the Czech Republic and Slovakia in 1993, remember.
Petr Nedved, Czech Republic: The 41-year-old winger has played six seasons in Czech's top professional league; in 48 games with Liberec Bili Tygri HC, he had 20 goals, 33 assists and a league-leading 151 penalty minutes. That last number is bizarre; good luck finding any other guys with 99-point seasons under their belt who wind up averaging more than three PIMs a game. For the record, two guys have accomplished that in a single NHL season—Kevin Stevens in 1991-92 and Rick Tocchet in 1992-93.
Nedved, the eighth-leading all-time scorer among Czech players, was one of the last to defect from the former Soviet Union, then played for seven NHL teams. He's best remembered for his time with the New York Rangers—and his contract disputes.
Jozef Stumpel, Slovakia: Also 41, Stumpel spent last season with Slovakia's Nitra MHC, totaling 13 goals and 37 assists in 52 games. He was done in the NHL when the Florida Panthers waived him in 2008. At his best, with the Los Angeles Kings (and Palffy, a close friend), Stumpel was a point-per-game player. He's the seventh-leading all-time scorer among Slovakian players. Sixth: Palffy.
Martin Straka, Czech Republic: He peaked as an NHL player in 1995, when he had 95 points for the Pittsburgh Penguins, largely on a line with Alexei Kovalev and Robert Lang. He also wound up playing—again—alongside Jagr with the Rangers from 2005-08 before leaving for Pizen HC of the Czech league. He had 54 points in 2012-13.
Martin Rucinsky, Czech Republic: A first-round pick by the Edmonton Oilers in 1991, Rucinsky had 612 points in 961 NHL games and peaked in 1995-96 with the Montreal Canadiens, when he had 25 goals and 35 assists in 56 games. Last season, with HC Litvínov, he had 13 goals and 14 assists in 33 games.
Radek Bonk, Czech Republic: The third overall pick in 1994 by the Ottawa Senators, Bonk took a handful of seasons to develop into a 70-point scorer, a mark he hit in 2000-01. He also played for the Montreal Canadiens and Nashville Predators before leaving for the KHL in 2009. He's spent the last four years with Oceláři Třinec of the Czech league and had 40 points in 39 games last season.


text:
Discussion in 'Trek Literature' started by Brian, Aug 5, 2008.
Oh sure, if your going to bring up specifics use facts and logic, well then, sure....
Seriously, in broad general terms, I don't see where the Kirk commanding the Excelsior and Spock commanding the Surak between ST 3 & 4 is contradicted by the dreaded canon. The only thing ever established about the time in between the movies is that they were in exile on Vulcan for three months before the begining of TVH, which works just as well being three months after the The Doomsday Bug as it does being three months after TSFS.
And I agree that the coolness factor of those stories really is the only important thing.
...which admittedly didn't stop my teenaged self from going through all sorts of mental contortions trying to make it all fit. ("Right! Saavik's pon farr-induced attack on the Enterprise blew open exactly the same parts of the hull that Khan's did, and that's why Kirk's suddenly depressed about Spock's death again in STIII. Absolutely!")
I wasn't quite convinced by the lead-in to Star Trek III, so at the time, I wrote my own story that went in-between issue #8 and Star Trek III. It wasn't very long -- maybe fourteen or fifteen hand-written pages.
The Klingon War in issues #1-4 was an attempt by the Klingons to take Genesis by force. When it failed, they turned to subterfuge. Kruge was a cousin of Koloth's, and he wanted revenge for Koloth's death. So, with the aid of Klingon spies, he faked intelligence that the Klingons were going to take the Regula-I station, while the real attempt to take Genesis would do so by gaining the secrets of the Genesis Planet. (The idea was that Starfleet would be more willing to defend the space station than they would be to defend an evolving planet.) The Enterprise took heavy damage, but managed to fend off the Klingon attack on Regula-I, and the ship was so damaged (since it was much more damaged in Star Trek III than it was at the end of Star Trek II) that they had to offload characters like Bearclaw, Bryce, and Konom while the ship limped home.
It wasn't very good. I wrote it when I was 11. But I thought it was a better continuity implant than what we got, and it made the events of Star Trek III as much a continuation of Mike Barr's early stories as it was a sequel to Star Trek II.
Now I'm regretting not polishing this up and sending it in to Strange New Worlds... :/
This is considerably more pimp than I thought. For whatever reason I had in my mind how sucky it would be to have comics on a DVD that you'd watch on a TV (the menu navigation alone would be terrible). Little did i know they were data DVDs. NICE.
Now the egregious lack of the X-Men/ST cross overs is a huge pile of SUCK for me, because those are the ones I care most about. (They are in fact the only ST Comics I've ever read) and I did so based on the fact that they tied into the novel Planet X so well. I'm sure the problem has something to do with licensing and being able to distribute the X-Men characters, but still.
Nevertheless I may just get this so I can finally see what the hype is all about with the Trek Comics.
Alex Fletcher at TrekMovie.com says the X-Men comics are supposed to be included, even though they aren't listed in the flap (see comments #45 and #75).
Ooh, look at comment #54!
So it may still happen. I hope it works out. Although I hope that set doesn't cost as much.
Hey, can anyone tell me which comic featured Janice Rand? I read the synopsis on... Memory Beta, I believe... and it sounded cool!
I can only think of a couple of comics featuring Rand, aside from some of the early Gold Keys (including the first issue, where the colorist misinterpreted her beehive as a wool hat and colored it red). She was prominent in issue 12 of Marvel's 1980-81 series, "Eclipse of Reason" by Alan Brennert and Martin Pasko. And she was featured in the fifth annual of DC's 2nd TOS series, "The Dream Walkers" by Michael Jan Friedman.
That usually means a different company will be distributing to international markets. Try asking your local comic shop to order it in for you.
Sounds like you have lots to catch up on then. Many of them were much better than the X-Men crossovers.
Thanks, Chris! "Dream Walkers" was the one I was thinking of.
So... an artist thought her 'do was a HAT? And made it RED?
^ "That's the most impractical, high-maintenance hairstyle for life aboard ship I've ever seen. Maybe she should just wear a hat."
To quote Kirk, "I know, but really...."
By the way, Vonda's Enterprise: First Adventure indicates that Rand wore her hair like that a kind of rebellion against her past life as a slave. (i.e., she wasn't allowed to let her hair grow, or style it, back on that planet.)
Maybe it's also a way of expressing her confidence. ("What the heck. If I can handle a style like this, I can handle a lot.")
Well, hey! A lot of times, movie-makers have sci-fi babes wear their hair in the most rediculous ways. Remember Princess Leah's cinnamon buns?
But then, I've seen girls wear worse....
The artists who did the early issues had never seen Star Trek. They lived in Italy, I think. All the artists had to go by was photo reference. Also, keep in mind that the line art and coloring in comics are done by different people. The colorist might never have even seen the photo reference that the penciller saw. He most likely just got line art of a woman with a big conical woven thing on the top of her head, and since he'd never seen the show or the reference photos, he must have concluded that it was a wool hat. I mean, really, to the uninitiated, that's a more plausible thing for it to be than some bizarre, ridiculously overcomplicated beehive hairdo.
Keep in mind that this was the '60s, while the show was still on the air. Nobody had any idea that ST would become some big media franchise that was intensely analyzed to its smallest detail, or that the comics they published would be preserved on digital media four decades later. To them, they were making disposable magazines based on disposable entertainment properties. It was an assembly-line thing, without the kind of care and devotion that gets put into today's Trek comics.
Come to think of it... do any comic writers ever imagine that "one day, the first editions of my comics'll be worth a cool million"?
I wonder if it ever entered the mind of Peter David....
Actually, there's a lot more visible soot and damage in ST III (both on the bridge and to the hull) than we saw at the end of ST II.
You're thinking about "Hardware Wars". The buns were eaten off Princess Anne-Droid's head by Chinchilla the Wookiee Monster.
Of course in the same issue, Spock's "solution" to a problem is as follows:
Funny and terrible at the same time
It should be interesting to read some of these old timers when I get the DVD but I am glad I never bothered buying the printed collections.
Separate names with a comma.


text:
I have an uncomplicated, five-step approach. It might be helpful to you, especially if you have a small budget and no access to fancy dashboards.
Before I get started, though, I have a few don'ts:
I ranted expounded on some measurement no-nos over at Kimber Media's blog. Go have a read.
Now that you've read the don'ts, here's what to do.
1. Identify the business objectives for your program/campaign.
Based on these business objectives, what do you need people to do? For example, if your objective is to increase sales, you need people to buy more of your product.
While increased sales is a common example, your objective could be anything. If you work for a nonprofit organization, your objective might be to increase members of an online community. An online community has people you can convert into members/donors.
That last bit is the most important. All roads might not lead to Rome, but they should lead to your business objective.
Your business objective(s) should be at the core of your measurement program. Before you do anything else, figure them out.
2. Identify how you will measure the success of these objectives.
If your objective is to increase sales, what percentage do you want them to increase by during your chosen time period?
If your objective is to grow your email list—that is where you convert the most prospects into customers—how much do you want it to grow? In what time frame?
Be as specific as you can. Don't just quantify what you want to achieve, but identify the time frame in which you want to do it.
3. Outline your communication strategy.
The most important thing to remember is that you shouldn't plan your strategy first. You should look at your business objectives and then decide how to use various communication vehicles to achieve those objectives.
In order to do this, you must understand how your audience reacts to and uses different communication mediums. Your program will be more effective if you include those mediums in your plan.
Don't seize the shiny, new toy du jour and say, "Oh, everyone's on ____! We're going to use _____!" Or, "Let's make a viral video like KONY 2012!"
You don't have to be everywhere all the time. Be where it makes the most sense.
4. Figure out how you will track your efforts.
This part is a lot of fun for me. With so many of our communications moving online, it's possible to track a lot of things—and I mean a lot.
Don't get caught up in tracking nonsense numbers. You might get an ego boost when you see your number of Twitter followers grow, but if that growth won't help you reach your business objectives, what's the point?
Tracking URLs are commonplace now. Use them. Get comfortable with Google Analytics and Google's URL Builder. You will be amazed at how much insight these tools give you, particularly when it comes to understanding what drives actions, clicks, downloads, purchases, sign-ups, etc.
This insight makes your strategy more informed and effective. It will also tell you what's not working so you can decide whether it's important enough to fix. Smart tracking lets you know what worked so you don't have to guess.
5. Correlate.
Even if you're not a statistician, you probably already know about correlation. Correlation is when one thing is related to another in a way as to have an effect on it.
For example, say you get a terrific hit on a blog you know is popular with your target audience. If your site's traffic increases more than usual, there is probably a correlation between the two.
Keep track of your outbound activities—new blog posts, news releases, email campaigns, and so on—and see what effect they have on your desired outcomes. (This is why you need to get comfortable with tracking.)
You should keep track of everything anyway so you don't mistakenly draw a correlation where there isn't one. Most of the time I don't have access to fancy dashboards, so I've found the easiest way to do this is to combine a few things:
If you keep an eye on everything you do, and also look at the back end, you'll be able to tell what works and what doesn't.
And, if you want to find the statistical correlation, Excel has a formula to help you out. I'd suggest you work with someone who is really into statistics to make sure you understand what you're doing.
That's it.
Put these five steps to work, and I'm fairly certain you will take giant steps in demystifying measurement. Who knows, you might even have fun!
Shonali Burke runs a successful agency of one, is the founder of the popular #measurePR Twitter chat, and Adjunct Faculty at Johns Hopkins University's M.A. in Communication program. A version of this article first appeared on Waxing UnLyrical.
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text:
The discs utilize TDK's exclusive DURABIS technology to offer legendary durability. TDK's proprietary printable surfaces yield the highest quality output from a range of devices including Microboards, Microtech, Primera (including the Primera Accent II Disc Laminator), Rimage Everest and TEAC. The BD-R25PWX25CB for inkjet systems features a blank, matte white surface, and the BD-R25THX25CB for thermal systems features a blank, glossy white surface. Both allow printing all the way to the disc hub, to maximize visual impact.
"With the launch of these two new Blu-ray Discs, TDK is reaffirming its role as a leader in the format's advancement," commented Sethu Palat, TDK Director of Marketing. "TDK's new 25GB BD-R printable surface media products deliver the exemplary performance and durability required for professional applications. Not only do the discs incorporate TDK's award-winning recording and hard coating technologies; they also offer optimal printing results with specially formulated print to the hub surfaces designed for inkjet and thermal systems."
As a leading developer of Blu-ray Disc technology, TDK has realized many of the format's greatest innovations. From pioneering the spin coating manufacturing method, to developing DURABIS, the world's first hard coating formulation, TDK has proven its leadership time and again. With the introduction of leading quality printable surface recordable Blu-ray Discs, TDK has reached another milestone.
TDK 25GB BD-R printable surface Blu-ray Discs are exclusively manufactured at TDK's state-of-the-art technical center in Japan. The use of this facility helps ensure ultra-stringent quality control. For commercial recording applications where errors can't be tolerated, TDK printable surface Blu-ray Discs are the wise choice. TDK's Blu-ray Disc media is widely recognized for its unsurpassed performance and quality.
At the 2007 Consumer Electronics Show, TDK received a CES Innovations Design and Engineering Award from the Consumer Electronics Association (CEA), honoring TDK 25GB BD-R Blu-ray Disc media. As the only media company that is a founding member of the Blu-ray Disc Association and a Blu-ray Disc Association Board of Directors member, TDK continues to play a key role in the format's advancement.


text:
The Rugby Championship
De Villiers demands better from Boks
ESPN Staff
August 30, 2012
Jean de Villiers' side battled back for a share of the spoils against Argentina in their most recent Rugby Championship outing © PA Photos
South Africa captain Jean de Villiers has demanded his side to bounce back to top form in the Rugby Championship after escaping with a 16-16 draw in their most recent outing with newcomers Argentina.
The Boks, who opened their campaign with a 27-6 victory over the Pumas in Cape Town, will hope to get their title challenge back on track against Australia in Perth on September 8 but De Villiers is aware to that his side must eliminate the mistakes that punctuated their hard-fought draw in Mendoza.
"We need to learn from our experiences, and we're growing as a team, but you can't keep making the same mistakes," De Villiers told reports following the Boks' open training session at the Orlando Stadium in Soweto.
"By Saturday next week we will know if we've stepped up or not. We're building as a team. Our discipline needs to improve, our execution, and our set-phases have taken a step backward. We maybe need to improve 40 percent but the margins are very small in Test rugby."
But De Villiers is not dwelling on the errors and insists there were positives in the way that his side battle back from a 13-3 half-time deficit against a fired-up Argentina who were making their home debut in the competition. "We've put that game behind us. Looking at the game again I feel better about it," he said.
"There were actually a lot of positives from the game. We believe we were a better team than how we played on Saturday. Even though there has been a lot of criticism of our attacking play, I thought that was actually quite good. The attack was quite good at times.
"Once you don't live up to expectations, you will be criticised," he said. "It's about the way you handle it. We have to be honest with each other and take it on the chin and believe in what we're doing. You can't take everyone's opinion to heart.
"It's a new coach trying to get a message and gameplan across to the team. We will all stand up for what we believe in. We believe in the coach and what we're doing is right."
© ESPN Sports Media Ltd.
Communication error please reload the page.


text: Final Jeopardy: write down a bet (in beer shots) after finding out the category. Write down your answer during the alotted time. If you are right, but someone is also right and wagered more than you, you must drink the difference. If you are wrong, you must drink the sum of your wager and the wagers of all those who got it right. If you play with a lot of people and this happens, one might have to drink between 25-50 shots, which is pretty damn hard and takes forever.


text:
On Twitter, the retweet is the ultimate "hey, look at this!" Retweets are an acknowledgment, in most cases an appreciation (but not always), of the original tweeters' comment.
Twitter keeps track of the retweet count. And like high school, getting retweeted can be a bit of a popularity contest.
So here they are. Some witty and clever and edgy. Others banal and self-serving. Let's countdown the top ten most retweeted tweets of 2010!
s***mydadsays Justin
Don't focus on the one guy who hates you. You don't go to the park and set your picnic down next to the only pile of dog s***.
June 28, 2010
rihanna Rihanna
Justin Bieber just flashed me his abs in the middle of a restaurant! Wow! He actually had a lil 6 pack! Sexy, lol! #Beliebersplzdontkillme
Nov 15, 2010
kanyewest Kanye West
I'm sorry Taylor.
Sep 4, 2010
ladygaga Lady Gaga
I'm beautiful in my way, 'cause God makes no mistakes. I'm on the right track, baby. I was Born This Way.
Oct 21, 2010
joejonas Joe Jonas
I cry because I love Justin Bieber!!!
Feb 27, 2010
alqaeda Al-Qaeda
Just noticed Twitter keeps prompting me to "Add a location to your tweets". Not falling for that one.
Sep 14, 2010
justinbieber Justin Bieber
Te quiero mucho mi amor.
July 11, 2010
liltunechi Lil Wayne
Aaaaaaahhhhhhmmmmm baaaaakkkkkkkkkk!
Nov 4, 2010
drakkardnoir Drizzy Drake
We always ignore the ones who adore us, and adore the ones who ignore us.
Sep 18, 2010
StephenAtHome Stephen Colbert
In honor of oil-soaked birds, 'tweets' are now 'gurgles.' http://bit.ly/cIhZNf
June 16, 2010


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This Holiday Season, Watch Out For Gout
Does the bedsheet touching the top of your foot feel like a hot prod? The season of overeating is almost upon us, and, Dr. Rock Positano warns in his HuffPo blog today, if you don't limit your gravy intake, you're asking for trouble in the form of a burning, swollen toe.
We're talking about gout, also known as "the disease of kings." And what is gout, exactly? This is a question many of us were first forced to confront when we heard that former Yankees pitcher/awesomely fun drunk David Wells had contracted it. According to Dr. Allan Gibofsky, a rheumatologist and professor of medicine at Weill Cornell Medical Center in New York City, "gout is found exclusively in humans and is characterized by an elevation in uric acid, known as hyperuricemia. It is a metabolic problem resulting in either overproduction of uric acid or reduced removal of uric acid from the blood."
Benjamin Franklin, Thomas Jefferson, and Jared Leto have also suffered from this painful condition, and if you want to avoid their fate control yourselves next Thursday, people. Turkey is a notorious trigger.


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Host a Chocolate Bliss Party
If the thought of someone sending you a box of chocolates and telling you to host a party sounds like a fun plan, then you might want to check out Hershey's new scheme. To promote their new line of Bliss chocolates, Hershey's is selecting 10 thousand folks to host Hershey's Bliss Chocolate Parties. If you're chosen to be a hostess, Hershey's will send you a party pack full of dark, milk and meltaway chocolates, as well as various host gifts. If you're interested, all you have to do is apply. The parties will be held on April 25, 26 and 27.
The new chocolate line launches this month, and while I haven't tasted it, Hershey's is calling it satisfying and sophisticated. The shape and size make me think of Dove chocolates, but I guess I'll have to go to one of these parties to find out.
So tell me, are you interested in hosting a chocolate Bliss party? If not, then are you interested in trying the chocolate? Or do you think it's going to be the same old same old Hershey's?


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Here are some of the notable quotes from around Major League Baseball this week:
"It was fate, one of those coincidences that happen once in a while. It was like being almost back home with all the mascots, the band playing, the Hurricanes giving the C-A-N-E-S. It was a real nice experience being out there. It was perfect timing."
-Rookie Gaby Sanchez on hitting his first Major League home run on the night the Marlins were honoring the University of Miami, Sanchez's alma mater. (South Florida Sun-Sentinel)
"I don't have any friends in Chicago, so [I thought] maybe this was a wrong [number], and I just ignored it maybe for two hours."
- So Taguchi, Chicago Cubs outfielder, who was recalled on Tuesday from Triple-A Iowa to join the Cubs. (MLB.com)
"Worst-case scenario [is] to be in the Minor Leagues, but the best-case scenario is to be in the same position I'm in right now. If this is my role from now until I'm 30, that's fine; if this is my role for the rest of my career, that's fine, too."
- Rusty Ryal, Arizona rookie, on the difference between being a utility player in the Major Leagues compared to an everyday player in the Minors. (Arizona Republic)
"I think he's been everything I expected him to be and maybe taken the extra step. I think with a first-year pitcher, you hope he lives up to what you expect from a first-year pitcher. I think what he's done is lived up to what you would expect from a third-year pitcher."
- Hall of Fame pitcher and current Braves broadcaster Don Sutton on Tommy Hanson. (Atlanta Journal-Constitution)
"From my 12th birthday 'til my last birthday last year, every single time I've wished that I get to the big leagues. I said, 'I want to be a Major League Baseball player.' I never made another wish. Not once."
- Ian Desmond, Nationals rookie. (Washington Post)
"Whatever it takes. I saw Casey [McGehee] wasn't there yet, so I grabbed it and went to the bag. I saw the end zone and dived for it."
- Milwaukee reliever Todd Coffey, after picking up a slow roller and diving headfirst to tag third base for an out on Saturday. (Milwaukee Journal Sentinel)
"No, I don't think I'm going to go from this stuff to being 25 again and knocking the catcher over."
- Randy Johnson declaring their was no need for further rehearsals after a 50-pitch batting practice session on Monday. (San Francisco Chronicle)
"It started even before he took over in center field, and it hasn't stopped. As soon as he's on base, it changes everything for the pitcher. And our guys know that the more attention the pitcher is paying to Rajai, the less attention they're paying to the hitter. They know they're gonna get something good to hit, and they've been taking advantage of that. It's been awesome."
- Brad Ziegler, A's pitcher, on the impact of Rajai Davis' speed on both the opposing pitcher and the rest of the A's lineup. (MLB.com)
"It's impossible that we're going to have the exact same team members as this year next year. So, to get to enjoy this moment now with them ... makes me very joyful."
- Ichiro Suzuki on the beer shower given to him by teammates, led by Ken Griffey Jr., after he recorded his ninth straight 200-hit season.. (Seattle Times)
"Just like I do with everybody who's young in this game -- it can be from my team or the opposite side. I just want everybody to progress and have a great career. He's one of the guys right now; he's one of the best shortstops in the game. He has a lot of future if he can stay healthy."
- Albert Pujols, on his mentoring of Hanley Ramirez. (MLB.com)
"I don't really know. Sometimes, I feel like I have an advantage. It should be more of an advantage for the pitcher, but it's a matter of constant adjustments."
- Zack Greinke, pondering the advantages and disadvantages of being familiar with a certain player. (Kansas City Star)
"It was nice and smooth. I threw sliders, changeups, everything -- good day at the office."
- J.C. Romero, Phillies reliever, who continues to rehab from a strained left forearm, after his bullpen session on Wednesday. (MLB.com)
"I didn't feel anything at all for two, three hours. I fell asleep, and I felt like I slept for 24 hours, one day. That was great. [The procedure] was a little scary."
- Alfonso Soriano, after having arthroscopic surgery (left knee) for the first time in his career on Tuesday. (MLB.com)
"I felt OK. I didn't have any pain. The arm strength wasn't quite where I want it to be, but I expected that. I did try to let a couple fastballs go, and not much happened. But I wasn't holding anything back. I wasn't trying to baby anything. I'm happy I passed the test."
- John Maine commenting on his start on Sunday against Philadelphia -- his first appearance since June 6. (Newsday)
"You can play through the pain if you feel like you're helping the team win ballgames, but when you go out there and you don't feel like you're helping anyone, that's when you take a step back and figure you're doing more harm than good. We took a look at it and found out there was something in there."
- Justin Morneau, who will miss the rest of the season with a stress fracture in his lower back. (St. Paul Pioneer Press)
"It's getting better. I can't circle a date just yet, but I'm getting to that point. Every day it's getting better, and I haven't had any setbacks. I would imagine, within the next day or two, I'll have a date circled of when I come back."
- Michael Young who has been out with a strained left hamstring. (Fort Worth Star-Telegram)
"Team speed really helps. When you have a chance to push the envelope and force the action, errors happen. Sometimes bad throws happen and you score cheap runs, and that's what we did tonight."
- Mark Teixeira commenting on teammate Brett Gardner, and the speed he provides the team. (Newsday)
This story was not subject to the approval of Major League Baseball or its clubs.


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Discussion in 'Science Fiction & Fantasy' started by Turtletrekker, Jul 2, 2010.
Usually, for big releases like this, we do a new thread.
Well, since I appear to be the first one to see it, any objections to me starting it?
Edit: Well, now it doesn't matter because I've started it.
As long as it's a spoiler free review I don't mind
are these people desperate?
The studio has released a 25 minute preview.
According to AICN it was fan made.
sorry i Did not know
It was fanmade. Sony already asked the author to take it down, which is pretty dumb when you think about it. That was free advertising!
Mods-- This thread can be closed, unless a spoiler-free haven thread is still wanted/needed.
Separate names with a comma.


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Read the full story here Web Link posted Friday, April 11, 2008, 12:00 AM
Town Square
Outrage at Almond
Original post made on Apr 11, 2008
Read the full story here Web Link posted Friday, April 11, 2008, 12:00 AM
Comments (3)
a resident of another community
on Apr 11, 2008 at 9:16 am
My complete comment was:
- There is also the question of relevance -- I wouldn't take Joe McCreary (or any Los Altos principal for that matter) and suggest that they would be a great principal at an inner city school. There experiences don't translate. So when we are asked to welcome a principal from a school with significantly lower test scores, why would the board be surprised that we're skeptical? Understanding HOW to work in the Los Altos environment is critical. Joe's 1 year of experience in this environment is probably worth 5-6 years of working someplace else. We face different challenges here. Check the test results for yourself...
Web Link
Web Link
Please note that this doesn't mean we wouldn't accept a teacher from a lesser-scoring school. In reality, we're in the very top 1% of the state, so we'd almost have to choose someone from a lesser scoring school. But the question would be, what has the measured impact of that individual be. In this case, it doesn't seem to be trending to the positive.
a resident of another community
on Apr 11, 2008 at 12:09 pm
I agree with Mr Goines that the communication process was inadequate.
However, I disagree on his statement that "...it depends on the community pulling together".
The Los Altos School District benefits from a terrific community providing countless of hours of volunteer time, extra programs only possible via the community dedication and generosity. In fact, a sizable extra source of revenue for the District comes directly from that same community (donations to Los Altos Education Foundation, PTAs...).
Year and year the community "pulls it together" and there is no reason it won't continue.
However the onus is really on the district and the board to reconnect with its community and rebuild some bridges badly damaged by its (often poorly communicated) decisions over the past year.
There is also some urgency for the District and the Board to rebuild these bridges as the District faces a pretty dire budget situation and will need all the community support to go pass these budget issues.
a resident of another community
on Apr 12, 2008 at 7:57 am
Here are two articles from the Palo Alto Daily News on the same subject:
April 9, Los Altos school board vows improved communication
Web Link
April 11, Almond principal: I never sent out furor-sparking letter
Web Link
Apparently a Board member sent to the Palo Alto Daily News, a letter that was never vetted by the principal nor even sent to the parents. the "letter" suggested that the parents stop their protests.
This type of communication is straight out of the Soviet Era Politburo's playbook.
What's next? Televised "Confessions"?
Burger chain Shake Shack to open in Palo Alto
By Elena Kadvany | 16 comments | 4,520 views
The Cost of Service
By Aldis Petriceks | 1 comment | 1,023 views
Couples: When Wrong Admit It; When Right; Shut Up
By Chandrama Anderson | 0 comments | 505 views


text:
Shane may be entitled to rejecting my comments, but he is definitely not entitled to editing them. It may be his blog, but it's my content - and my ideas. Misrepresenting my ideas on purpose is a violent act.
I can tell you are confused. You are asking all the wrong questions. The real question is: why did we let this bunch of crazy people drive perl directly to the garbage? You don't care that it's become a joke? Interesting.
You, as member of the perl community, are also responsible for it. Take some resp
Misrepresenting my ideas on purpose is a violent act
Bullshit. You are insulting every victim of true violence, ever.
Why not go all the way and call it rape? chromatic raped you just as much as he was violent to you.
If it is valid, then what it says about my post is also valid about yours, hence rendering your comment invalid.
If it isn't valid, then it can be safely ignored.
Our Perl community leaders have failed us, and it's up to us to grab the bull by its horns.
Why don't you? Put forth a credible proposal and people will follow.
Go ahead.
I will start a programming language eventually; have a few important clients to keep happy at the moment, but I've promised myself I'd do it.
I will not put forth a proposal for the Perl community, though - they are oblivious to anything that doesn't fit their preconceptions - such as my "preposterous idea" that the number of operators in Perl 6 (and IMHO perl 5 too, but primarily Perl 6) should be minimised. Making Perl 6 for mortals is n
Sounds to me like the community and the leaders fit together fine… for good or for ill. All that puzzles me is why you'd care to continue to rail against it to no apparent end (since if that is how the community is, and that is how the leaders are, and the two fit together – then you aren't going to effect any change).
s/the community/what remains of the community/
All that puzzles me is why you'd care to continue to rail against it to no apparent end
Contrarianism, basically - someone has to do it. It usually keeps communities honest - although crying over how much of a troll a particular contrarian is. See Christopher Hitchens, for instance.
So you're doing it for the good of the community. How kind of you.
Is there something we can do to donate this kindness to another community?
Why exactly does it bother you? Are you afraid I might be right in something? If I'm just a lone nut, what difference does it make what I post?


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I have gotten into the habit of using std::vector for a lot of things. However I wanted to ask why in the world the STL designers made removing items from a vector invalidate every iterator/index after the removed item? This is a major drawback to an otherwise extremely nice container.
The problem of invalidation though makes a vector useless in certain instances. I've looked for a container that allows array like access and does not invalidate iterators when objects are removed. One could use the vector as a recycling array that did not remove the actual item from the vector but rather nullifed the data. The iterator would then be stored as a cached iterator and the add() function would then look at this. If it was null or invalid it would add to the end, if it was valid it would add where the last 'removal' was done. This does not require an invalidation of items beyond the 'removal' point.
I've looked at other container types in the STL and just cannot find a good fit for what I need. I don't want a static array because I don't want to iterate the entire array if only 5 elements are valid in the array. Yes I could code my own container type and have already, but I was wondering if there was an STL equivalent?


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Election night saw landslide victories for incumbent Republicans like Gov. Mike Johanns, and continued the slide of Nebraska's Democratic party.Tobias examines the struggles and future of Nebraska's Democratic party.
live streaming schedule & on demand videos
|09:00 am
|Unicameral: Legislative Session
|Nebraska Legislative Session
|12:00 pm
|Legislative Hearing: Executive Board - Room 2102
|Nebraska Executive Board
|01:30 pm
|Legislative Hearing: Business and Labor - Room 2102
|Nebraska Business and Labor
|01:30 pm
|Legislative Hearing: Transportation & Telecommunications - Room 1113
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|Legislative Hearing: General Affairs Committee - Room 1510
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|Legislative Hearing: Education Committee - Room 1525
|Nebraska Education Committee
|01:30 pm
|Legislative Hearing: Appropriations Committee - Room 1524
|Nebraska Appropriations Committee
|09:00 am
|Unicameral: Legislative Session
|Nebraska Legislative Session
|01:30 pm
|Legislative Hearing: Agriculture Committee - Room 2102
|Nebraska Agriculture Committee
|Backyard Farmer: Lifestyle Gardening, 502
|Lifestyle Gardening host Kim Todd focuses on a wide variety of gardening topics including getting...
|Backyard Farmer: Lifestyle Gardening, 501
|Lifestyle Gardening host Kim Todd focuses on a wide variety of gardening topics including getting...
|Making the Game Safer
|Safety in the game of football has been a hot topic lately, especially in the high school ranks...
|Nebraska Stories: Talk to Me and More, 902
|"Talk to Me" Lincoln musician and singer Josh Hoyer reflects on his recent stint on NBC's "The...
|Nebraska Stories: Talk to Me, 902
|Lincoln musician and singer Josh Hoyer reflects on his recent stint on NBC's "The Voice" and on his...
|Nebraska Stories: Kearney Goes to War, 902
|During World War II, thousands of people came to Kearney for training before leaving for combat in...
|Nebraska Stories: No Filter, 902
|Husker volleyball standouts Briana Holman and Sydney Townsend traveled to Nicaragua with other...
|Nebraska Stories: Plein Air Poet and More, 901
|"Plein Air Poet"
|Last Shot for the Royals
|The women's basketball team at tiny Grace University in Omaha is having a decent 2017-18 season....
|Big Red Wrap-Up: Post-Season Special, 1716
|Join our hosts as they review 2017 and look ahead to year one of the Scott Frost era. Original Air...


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- Buy New
- Buy Open Box
- Alerts
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Voice coil ˜ 1.5" (32 mm)
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Number 1
Type X-ART
Diaphragm area 4 inch² (2420 mm²)
Equiv. Diaphragm ˜ 2" (56 mm)
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Magnetically shielded No
Height x Width x Depth 11.5" (290 mm) x 7" (175 mm) x 8.5" (220 mm)
Delivery contents
Front cover, Cinch cable for stereolink in black or white, Power cord, Manual


text: I joined QCA recently and got a one year subscription to QNN. I signed up yesterday, and even though the total showed up as $0, they still wanted you to pay through PayPal. A little while later, I got an email from PayPal saying I set up an automatic payment to go on indefinitely, and I would be charged $24 yearly. I did go to PayPal and cancel the automatic payment. I just thought I'd let everybody know, in case they don't read the email from PayPal.


text: Trying to see if the USTA is leaning toward bumping up people or bumping down people. Last couple years there was a big attempt to bump people up. Several years before that there was an effort to bump people down. One woman I know who played #1 singles on her 4.0 team and lost most of her matches, but won a few was bumped down. She was one of the best players on her team which consisted of all 4.0 players. That seemed quite a bit different than the last couple of years where a record like that might have gotten you bumped up. Also, the #2 singles player on our team was not bumped up despite being undefeated during the season. Last year the #2 singles player (different guy) was bumped up after being undefeated (though I was not, playing #1 singles and only losing a couple of matches) Is there is propensity toward bumping people down now?


text: Any ideas on what I can do to add version control to VC++ 2005? Anyone tried it? Probably as an external tool accessible through the tools menu... but what would you suggest? CVS? Does it integrate well? I'm concerned with possible clashes with how C++ 2005 Express stores project files.