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I am fairly new to programming for computers. I have noticed that most of the C++ tutorials don't touch on the area of header files a whole lot. Does anyone know where a good header file tutorial is located?
I am fairly new to programming for computers. I have noticed that most of the C++ tutorials don't touch on the area of header files a whole lot. Does anyone know where a good header file tutorial is located?
Then, don't worry about header files or use them.I am fairly new to programming for computers.
Well I'm not really "new" to programming. I've been programming on TI calculators for about 5 years. By new I mean I've only been programming for computers for about a year, which isn't that long to me. I feel I have a fairly good understanding of the basics of C/C++. Sometimes I will look through the include files for Dev-Cpp and I find word and commands that are foreign to me, such as extern, the conditional statements that are preceded by the #, and all of the underscores. I was wondering if there was a tutorial that explains the process of making header files and libraries in detail. I am starting college and taking a programming class 1st semester and I would like to learn as much as possible before I start class as I'm taking and advanced calculus and would like to relieve some pressure.
Thanks for the help fellow C/C++ programmers.
I think this is a reasonable question. I often wonder what is actually happening when you include header files. I know that it is a means to access functions within the header file but it would be nice to get a more in depth of how the compiler acts upon the
also i noticed if you get into the VC98 folder in MSVC++ and look in the include folder there
you see all the different header files.
Can i learn anything by clicking on the various header files or not?
If i can then how about a tutorial on the subject .
Mark S.
I tried to make some guide about header files...
Maybe i'm not very good at english but try to understand something...
Quick guide to header files :
I'll give you one example :
This is your header file (.h).
Contents :
And you have main file (.cpp), where is the main function.Code:// file : myheader.h void foo() { // do nothing }
Contents :
myheader.h and main.cpp are seperate files.Code:// file : main.cpp #include "myheader.h" int main() { // call the foo function foo(); return 0; }
Lines that begin with '#' are preprocessor directives.
Preprocessor reads the code before compiler.
When it sees line that begins with '#', it executes the command on the right side from the '#' sign.
In our case this is #include "myheader.h".
'#include' tells preprocessor to copy whole contents of some file (myheader.h for us) to the file where the '#include' statement is used.
So, when preprocessor does its job, we have one big file that looks like this :
I hope this explained you some things about preprocessor and header files.Code:// combined file that preprocessor makes for compiler. void foo() { } int main() { foo(); return 0; }
If I made mistake somewhere, feel free to insult me.
You can download sample project here > Download
It should work on any newer compiler.
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Ok, let's have at it.I feel I have a fairly good understanding of the basics of C/C++.
Defining a variable as extern implies that it's defined somewhere outside the current scope. The extern keyword says to look outside the current block for the definition of the variable. If the variable isn't contained inside a block, then the search for its definition expands to the other files in your program.Sometimes I will look through the include files for Dev-Cpp and I find word and commands that are foreign to me, such as extern,
Those are called "preprocessor directives". The preprocessor's job is to prepare your code for the compile phase, whereupon the compiler will process your code into machine instructions. Learning all about the preprocessor directives is like learning another whole language. An example of a preprocessor directive is #define:...the conditional statements that are preceded by the #
#define pi 3.141
That tells the preprocessor to substitute the characters "pi" with "3.141" in any lines below the current line when the preprocessor encounters "pi" in your program(except inside comments or in string literals surrounded by double quote marks). You can also use #undefine, which says to stop making those substitutions for the lines of code below the line containing #undefine. If you do this:
#define pi
then "pi" will be replaced by nothing, i.e. it will be removed from the lines of code.
Another very common preprocessor directive is #include. That tells the preprocessor to substitute the contents of the specified file in place of the #include statement. With header files, you may also see the ubiquitous:
That hints at the fact that there is something more going on when you use #define. When you write:Code:#if !defined MY_HEADER_CONTAINING_MY_STUFF_123456789_H #define MY_HEADER_CONTAINING_MY_STUFF_123456789_H //C++ code here #endif
#define pi 3.141
pi becomes a "token", and when the token is found in your program it is replaced by 3.141. In other words, pi is some kind of entity that can be #defined or #undefined. That's important because in the line:
#if !defined MY_HEADER_CONTAINING_MY_STUFF_123456789_H
you are telling the preprocessor that if the token MY_HEADER_CONTAINING_MY_STUFF_123456789_H hasn't been #defined, then go ahead and continue looking at the next lines between #if and #endif. The next line is:
#define MY_HEADER_CONTAINING_MY_STUFF_123456789_H
That says to replace the token MY_HEADER_CONTAINING_MY_STUFF_123456789_H with nothing, i.e remove it from the rest of the lines of you program. You wouldn't expect to find that token anywhere in the lines of your program--all you want to do is #define the token. Once you #define the token, then the if statement will fail the next time you try to include the header file. The effect is that the lines of C++ code between #ifndef and #endif will not be included more than once in a file. Header files contain declarations, and if you include the header file more than once, you will include the declaration of a variable in your file more than once, which is an error. For instance, if you do this in a simple "hello world" program:
int num = 3;
int num = 3;
the compiler will say:
Other preprocessor directives:
#error -- terminates the preprocessing and displays the specified message. For instance,
#pragma -- an implementation defined directive, ignored if not recognizedCode:#ifndef myFile #error "Something went wrong with myFile" #endif
There are also standard macros defined by the preprocessor, e.g.
__LINE__ : the line number of the current line as an int
__FILE__ : the name of the source file as a string literal
I'm not sure what you mean. Underscores can be part of names. I think by convention leading underscores followed by a capital letter are used in C++ libraries, so you don't want to use variable names like that in your programs.and all of the underscores
Why might you want to put your declarations in .h files and your definitions in .cpp files? See this recent thread:
Remember when you include a .h file into another file, the include statement is replaced by the .h file, so any legal C++ you could type at the location of the #include statement can be contained in the .h file.
1) Name your file with a .h extension.I was wondering if there was a tutorial that explains the process of making header files
2) Put some inclusion guards in the file:
3) Put some C++ between the inclusion guards.Code:#ifndef SOME_UNIQUE_NAME_H #def SOME_UNIQUE_NAME_H #endif
4) Include the .h file wherever you want those lines of code to appear.
Dynamic link libraries(dll's)?? I will defer because I don't know how.tutorial that explains the process of making libraries
(...the above was garnered from "Ivor Horton's Beginning C++", chap 10: Program Files and the Preprocessor)
Last edited by 7stud; 08-04-2005 at 12:52 AM.


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"Once properties are redeemed by the original owner or sold at public auction, the funds collected are forwarded to the county, where the property is located, one year after the collection date," Thurston said. He and his office work diligently to collect this important source of revenue on delinquent parcels.
"Property taxes play a vital role in the stability of county revenues. Public schools and county services depend on real estate tax dollars to aid in funding their programs. Without these funds our communities may miss out on essential programs and growth," Thurston added.
The Commissioner's office works with county officials in all 75 counties to ensure collection of property taxes. "Our county officials are the boots on the ground workers in this process. We appreciate all that they do and we would not have collected these turnback amounts without working hand in hand with them on a daily basis," said Thurston.
Pope County received $263,964.78 in combined turnback funds during 2012. In total, Arkansas counties received $21 million from delinquent tax collections last year.

subset: commoncrawl


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OCEAN CITY — Today marks the day when the town of Ocean City takes back the take-home vehicles.
Depending on whom you ask, the quest to trim back the town's take-home vehicle fleet dates back a number of months or years, but now that the council's recent decision to cut 63 percent of employee take-home cars has gone into effect, the sting of that decision has not fully faded away.
"I know there's a lot of people who aren't happy about this," said Councilman Joe Hall, "but I really think that those people who are angry really need to get a good perspective on this issue and be happy that they still have their jobs in these economic times."
Hall's sentiments and stance on this issue seem to be the consensus when talking to some of his colleagues on the council.
Margaret Pillas, who not only was a huge proponent of scaling back the town's fleet of 71 take-home vehicles, but was also the most radical in her desire to take all of the vehicles away, said that she is still glad to see the decision is finally taking effect.
"I haven't heard about people being upset with this decision," said Pillas. "What I've heard is that people are glad we finally did something about these vehicles, and I truly believe that people are coming to terms with how bad this economy is, and this is just one of the things that needed to be done."
The council took City Manager Dennis Dare's recommendation to cut 45 of the 71 vehicles two weeks ago, thus saving the town of Ocean City almost $90,000. The vehicles will now be parked at their respective departmental headquarters and essentially used by the town employees during work hours and will be signed out for usage, much like one would sign out a library book.
The departments of public safety and public works were the two hardest hit in the resort, as 24 of the 45 cars came from those two departments, including the removal of cars from two captains, six lieutenants, one sergeant, a corporal and four detectives.
On the public works side, the department heads of water, wastewater, transportation, construction and the airport all have lost their vehicles. Six of those department heads boast combined service to the town of Ocean City to the tune of 119 years.
"If you look at it that way, I think people can understand why some of these gentleman are a bit upset because they almost feel like a promise has been broken," said Public Works Director Hal Adkins. "When these men were hired, or as they advanced, the vehicle became part of their package or agreement with the town so to speak, and now a part of that has been taken away."
Adkins said that although the employees that he oversees in the public works department are not happy with the decision to cut back the take-home vehicle fleet, he says that they understand why the city felt it needed to go down that path and make such a change.
Apparently, the decision has also sparked concern from the Fraternal Order of Police, who announced plans to file a grievance with the Labor Commissioner citing their displeasure with the town's lack of communication with the FOP before the decision.
Still, council members are sticking to their guns about the change, calling it a necessity, albeit a difficult one.
"I was very conflicted with this decision and I said that after we voted," said Councilman Doug Cymek, "but we have worked really hard to keep people working in the town of Ocean City, and 30 miles up the road, people are in turmoil and there are layoffs and furloughs. We have tried very hard not to do those things and keep our people working, and we are in a good financial situation in Ocean City because we made those $2.7 million in budget cuts last year."
Council President Joe Mitrecic agreed with Cymek, citing that the time had come for the "perks to be taken away."
"I have a hard time feeling sorry for someone having to give back their vehicle when they've got two or three cars of their own sitting in their driveway," said Mitrecic. "Why should the taxpayers of Ocean City have to pay the money? I know there is grumbling about this, but we are trying to maintain and sustain our way of life in Ocean City and sometimes we have to make tough decisions."


text: At 9:17 AM -0600 1/24/97, Jonathan Robie wrote: >At 08:28 AM 1/24/97 -0600, Carl W. Conrad wrote: >>At 5:58 AM -0600 1/24/97, Jonathan Robie wrote: >>>OK, I'm moving on to the next chapter! In Ephesians 2:1, hUMAS is accusative: >>> >>>Eph 2:1 (GNT) KAI hUMAS ONTAS NEKROUS TOIS PARAPTWMASIN KAI TAIS hAMARTIAIS >>>hUMWN > >>The accusative of respect, at least in traditional grammatical terminology, >>tends to be used of accusatives qualifying adjectives or verbs denoting a >>state to denote a thing in respect to which the verb or adjective is limited >>(Smyth, #1601); Wallace seems to be using the term in an idiosyncratic sense >>including limiting functions of an accusative noun that can't be explained >>otherwise. But Ephesians 2:1 hUMAS ONTAS KTL. is not an accusative of respect. > >I may be misunderstanding the accusative of respect. I was thinking that the >accusative of respect might lead to a translation like: > >"And as for you, you were dead in your sins and transgressions, following >the age of this world, following the prince of the powers of the air, the >spirit which now works in those who are disobedient." > >Is this wildly off the mark? Yes, I really think it IS "wildly off the mark"--at least as regards an "accusative of respect." In fact, there's something backward, cart-before-horse, so to speak, about thinking of it in terms of the translation it might produce. Here's a "classic" (as well as classical Attic) Accusative of Respect: in the Oedipus Tyrannos, Teiresias is told by Oedipus: TUFLOS TA T' WTA TON TE NOUN TA T' OMMAT' EI "Blind at ears and at mind and at eyes are you." The three accusatives are clarifiers of the adjective TUFLOS, "blind." They specify in what respect Teiresias is blind. In Eph 2:1 one might argue that hUMAS is an accusative of respect only if it relates somehow to an adjective or an intransitive verb that it helps to clarify--and there's nothing in what follows to link it to (which is why some people want to make this whole clause an "accusative absolute"), but as I said previously, I really think that hUMAS ONTAS KTL. in 2:1 anticipates and is resumed by hHMAS ONTAS KTL. in 2:5. The sentence is an anacoluthon--its syntax (in terms of its initial structure) is broken off and recommenced in 2:5. Carl W. Conrad Department of Classics, Washington University One Brookings Drive, St. Louis, MO, USA 63130 (314) 935-4018 email@example.com OR email@example.com WWW: http://www.artsci.wustl.edu/~cwconrad/


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Krystle Arzola says her husband was dedicated to helping young people get into college, especially those who would be the first in their families to attend, just as he was.
Art Arzola turned down an offer to fly to Humboldt State University so he could ride on the bus with 44 other high school students who were planning to visit the campus.
Arzola, 26, of Rancho Cucamonga, was one of the 10 who died in the head-on crash with a FedEx truck in the Northern California city of Orland.
"He said, 'No, I want to be with these kids on this bus,'" his wife said. "That's a long bus ride and I want to be with these kids."
Messages from students her husband mentored are helping Krystle get through each day, along with her faith.
Krystle Arzola said her husband was living his life's passion.
"His job was not to tell these students if they could go to college, it was to show them how to get there," she said.
Added Arthur's mother, Patricia Arzola: "That was his vision. He wanted to say, 'Hey, you have a shot. You can do it, too.'
"This is a really tough time for everyone."
The young couple shared a strong faith. Her husband's final journal entry are notes from a church service he attended four days before he died.
"He wrote this in bold," his wife said. "Storms don't last forever and remember that God will be with us throughout the storm."
Some $15,400 had been raised by Tuesday afternoon on the website gofundme to help pay for the funeral, set for Friday.
On Wednesday, mourners were planning a vigil at the University of La Verne Arzola, where Arzola was a graduate student.


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MORE McD's: Preview | Meyer's mail | West report
The 2011 McDonald's All-American Game festivities got rolling on Sunday afternoon. Players from the East and West gathered at the Attack Athletics facility in Chicago for their first practice session. For the East, Kentavious Caldwell-Pope, Brad Beal and Mike Gilchrist were among the first practice's standouts.
Kentavious Caldwell-Pope, SG- The thin Georgia signee has a lightning-quick release and deep range. Generally considered more of a shooter, he's a lot more clever and effective off the bounce than people might think. His lateral quickness and instincts are big assets.
Brad Beal, SG- The 6-foot-4 shooting guard looked at ease and comfortable playing under the watchful eyes of several NBA types who joined the typical crowd of analysts to take in practice. He looked to have some bounce in his step and was quick to the rim on baseline drives. As usual, the future Florida Gator was money from deep and the NBA three-point line wasn't an issue for him.
Mike Gilchrist, SF- The 6-foot-7 Kentucky signee was outstanding during the first session. His team had the chance to scrimmage quite a bit and he flourished on both ends. It was good to see him hitting dribble pull-up jumpers from either elbow extended out to the three-point line. Of course, he was active as a defender as well.
Chane Behanan, PF- Sometimes at an event such as the McDonald's game, shorter power forwards like Behanan can struggle to get their shot off. That's not the case for Behanan. He uses fakes, hits jump hooks and has range to around 20 feet. He picked his spots and was very effective offensively.
James McAdoo, PF- McAdoo was active in transition as he ran rim to rim for dunks and tips. He's got wide shoulders and great length, and is a pretty fluid overall athlete. Catching, facing and attacking from the free throw line, he's efficient.
Rakeem Christmas, C- There are times where the effort level isn't necessarily there with Christmas so it was a pleasant surprise to see him play with a high level of activity on Sunday. He ran the floor, contested shots and made guys work for hoops near the rim.
P.J. Hairston, SG- When it comes to shooting the ball from way beyond the three-point line, the 6-foot-5 Hairston may have the deepest range of anybody in attendance. At the 2004 game it was another North Carolina signee - J.R. Smith - who put on a shooting clinic. While Hairston wasn't draining it from 28-32 feet as Smith did, he was pretty good from 23-26.


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STAT's Back!
After undergoing preseason knee surgery, New York Knicks All-Star forward Amar'e Stoudemirewill make his season debut on Tuesday night against the Portland Trail Blazers, a league source told Yahoo! Sports.
Amar'e Stoudemire takes a practice shot before the Knicks' home game vs. the Lakers on Dec. 13. (AP).
>Whether or not Carmelo Anthony returns from his hyper-extended knee on Tuesday, the plan remains for Stoudemire to come off the Knicks' bench. Anthony told reporters that he will test his knee in pre-game on Tuesday and then make a determination on returning to the lineup.
Stoudemire, 30, has been one of the NBA's elite power forwards and had come into training camp believing he had worked himself into the best shape of his professional career. Nevertheless, Stoudemire suffered a knee injury in October, underwent surgery on Oct. 21 and has been rehabbing the injury most recently in practices with the Knicks' D-League franchise in Erie and finally with the Knicks.
"We need him," Anthony told Yahoo! Sports' Marc Spears on Friday. "I know we are playing well, but the more bodies, the better, especially at this point where guys are doing well. We are ready for him."
The Knicks are 21-9, leaders in the Atlantic Division and a half-game behind the Miami Heat for the best record in the Eastern Conference.
New York: The Knicks have enjoyed two days at MSG Training Center for rare practice sessions and the chance to rest and recover from a three-game west coast road trip last week. Carmelo Anthony practiced one of the two days in New York but did not participate in all of the drills on Monday afternoon. Head coach Mike Woodson said the MVP candidate is day-to-day and Anthony added that he will most likely make a decision close to game time on Tuesday night. Tyson Chandler and Jason Kidd received recovery days after the two players logged major minutes during the road trip. Raymond Felton is out 4-6 weeks due to a fractured right pinky finger. At 21-9, the Knicks are a half game behind the Miami Heat in the Eastern Conference standings and 5.5 games over the Nets for the Atlantic Division lead.
Portland: The Blazers entered the 2012-13 season with a new general manager and head coach. Additionally, Portland drafted one of the elite players in the NBA Draft by selecting Damian Lillard out of Weber State. Lillard joins a young nucleus that has aspirations of reaching the postseason after a 28-38 finish last year. This season, the Blazers have been up and down until a recent surge of five wins in seven games leading up to the matchup against the Knicks. Portland is now 15-14 overall and the contest in New York will kick off a four-game road trip.
Key Match Up
Definitely concerned about Amar'e's propensity to fall for fakes or leave his man against Aldridge. If he were to quickly get in to foul trouble, all the energy and expectation will be flushed down the proverbial lavatory.


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Please accept our condolances.
Thanks for reinvigorating this thread. This will be our first summer of retirement and we have plans:
April: Branson, Ohio, Chicago, the Spam Museum (a must do), Blackhills, Custer Battlefield and home to MT
May: Lolo, southern Idaho, Fraser River Valley thru British Columbia to Hyderfor Hyderseek, Jasper, Banff then south to Nakusp and the lakes region of BC.
June: Cassiar to Whitehorse, Skagway, Klondike Hwy to Dawson City for Dust 2 Dawson, Fairbanks, Manley Hotsprings, perhaps Homer for a wedding then the Alcan home.
July: National Rally, Yellowstone NP, Glacier NP
August: Daughter's graduation in Bozeman, Beartooth Rendezvous and Stanley Stomp in ID
September: 3 Flags Rally Check Point, state Airhead picnic in Kalispell, and perhaps the MOAB
We'll fill in the gaps riding the many great roads of our new home in Montana. By our estimation we'll log 25K miles or better. Can't wait to get started


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California has long embraced the notion of ensuring that parents have more school choice options. The Associated Press recently reported, for example, that California added 81 public charter schools during the 2012-13 school year, pushing the state's total number of publicly funded autonomous schools to more than 1,000 – the most in the nation.
But choice isn't simply about giving parents more options; it's about giving them more quality school options. Recently, StudentsFirst released our State Policy Report Cards, which assess to what degree each state's education policies are student-centered. We graded each state's school-choice policies based on how well parents can access great schools for their children and whether each state creates a policy environment for high-quality school choice options to flourish.
Unfortunately, California received a failing grade.
When it comes to public charter schools, many of them produce outstanding results and increase achievement among their kids – but that's not always the case. Accountability for student performance is key. This is why it's critical that California law establish a system in which charter schools must demonstrate high levels of student achievement in order to replicate or expand, and the highest-performing public charter schools are given priority for rapid expansion.
Also, California law does little to ensure authorizers hold their charters accountable for performance. State law does not require public charter-school performance contracts that hold each campus accountable for measurable student academic performance goals, and there are no clear requirements for closing charter schools that are failing kids.
Ultimately, California's charter accountability framework should help the highest-performing charters replicate, guide the middle-performing schools to excellence, and enable swift closure of the persistently low-performing.
It's also important to remember that charter schools are public schools, serving the same communities and the same school children as traditional public schools. As such, they should receive the same funding as their traditional public school counterparts.
Unfortunately, this is not the case in California. Last year, the California Legislative Analyst's Office issued a report showing that public charter schools receive at least 7 percent less funding than traditional public schools. For some charter schools, the resulting inequity can exceed $1,000 per student. That's why we were encouraged to see that Gov. Jerry Brown's current budget proposal aims to close this funding gap for public charter schools.
Eventually, with stronger school choice policies, California can create a public education system in which every parent – no matter where she lives or how much money she makes – can choose for her child to attend an excellent school.
Eric Lerum is vice president of National Policy for StudentsFirst, an education-reform group.


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UWG users,
After finishing the guide in the Searing Gorge, I found many places to farm certain items that have become scarce on the AH. First off, you have the road south of Stonewrought Pass on the hill with the outhouse where the Dark Iron Dwarves are. They have a surprising drop rate of Aquamarine (A jewel) which is bound to get you a quick 10 gold. Second, you have the Slag Pit. There are many things to farm in this area, for example, Mageweave Cloth (Now scarce in the AH). There are also Incendosaurs in the lower cave area and their scales are very valuable. You do not need to skin them to get the scales, they are in the regular loot window. Many Tailoring Patterns are found off of the Dwarves there, along with the occasional Crystals and Ores off of the slaves that defend the Dwarves.
After that, you have BlackChar Cave. The spiders have Silk that sells for quite alot now. As you farm the Silk, look out for Dripping Spider Mandible. You can sell these for a few extra silver at a vendor. You might find a few elementals on your way, which can get you a lot of gold if you happen to find Elemental Fire, or a Core of Elements.
FireWatch Ridge can get you some Mageweave and a few Tailoring recipes along with some nice rare weapons.
I hope these tips will make you a lot of gold like it did for me. I went from 200 gold to 285 when I sold all of these valuable materials.


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Dagmar Louise Linton
Missing since August 22, 1986 from Brinnon, Jefferson County, Washington.
Classification: Endangered Missing
Vital Statistics
Circumstances of Disappearance
Dagmar is missing with her husband, Robert Linton and they were last seen on August 22, 1986, in Brinnon, Washington. They retired from county jobs in Stockton, California and disappeared en route to Vancouver's Expo. They disappeared while camping on the Olympic Peninsula. A large, bearded white male with a bandaged right hand was seen using the Lintons' credit cards to buy several items.
They are believed killed by Charles T. Sinclair, a serial killer. His signatures matched those used to by things with the victims' credit cards. Property recovered that was bought with the couple's stolen cards were found in Sinclair's storage shed.
The Lintons' truck was later found at the Seattle-Tacoma airport.
Investigators Jefferson County Sheriffs Department
If you have any information concerning this case, please contact:
Jefferson County Sheriffs Department
Agency Case Number: 861363
NCIC Number: M-999192145 Source Information:
Please refer to this number when contacting any agency with information regarding this case.
California Department of Justice
Seattle Post-Intelligencer
Source Information:


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These are tumultuous times in Egypt, with massive protests followed by the military ousting the country's leader. This time it's Mohammed Morsi; a couple of years ago it was Hosni Mubarak being kicked out. In some respects, it's a scary sequel for South Florida's Egyptian community.
"The concern that I have is the safety of the people," said Father Timotheus Soliman, who leads the congregation at Miramar's St. John the Baptist Egyptian Coptic Church. He pointed out that just last night, Morsi called for the followers of the Muslim Brotherhood to resort to martyrdom as it became obvious the military would remove him from his elected position.
"He is calling for civil war," Soliman said. "Right now, not only Copts are in danger, but even Muslims, because whosoever is not in agreement with the philosophy of the Muslim Brotherhood is considered an infidel."
Father Soliman is worried about his family back home in Cairo, and about the future of Egyptian society. He said it's obvious from the huge protests against Morsi's tenure that many Egyptians are having second thoughts about the revolution that toppled Mubarak but allowed the Muslim Brotherhood to come to power.
"They are not regretting it, but they were sad that it was stolen from them," Soliman explained.
"Democracy is not just about being elected through the ballot box, it's also about allowing the voices of the people to be heard," said U.S. State Department Spokesperson Jennifer Psaki, referring to widespread criticism that Morsi was guilty of suppressing dissenting voices in Egypt. Father Soliman agrees, and hopes the next government there will represent everyone, including Muslim brotherhood supporters.
"They're part of the political game, but they need to take their portion, not to take everything," Soliman said.


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Marketplace Returns Next Weekend!
- Details
- Published on Wednesday, 15 January 2014 19:24
We are just over a week away from the 40th Annual ACCEL Banquet of Champions, which will take place the weekend of January 24 – 26, 2014 at Kalahari Resorts in Sandusky Oh.
A popular feature that takes place during the weekend is "Marketplace". Marketplace has proven over the last few years to be an excellent opportunity for vendors to connect with racers to discuss their products and services in casual non-racing environment. Vendors such as Summit Racing Equipment, Trick Flow, ACCEL Performance Group, Krystowski Tractor Sales, Wiseco Performance Products, Racers for Christ, Velvet Ice Cream, R & R Auto Body, Quick Fuel Technology, Foghorn Designs, Kelley Islands Venture Resort, NMCA/NMRA, KB Trailer Sales and Summit Motorsports Park tech officials will be on site Saturday January 25th from 10am to 3pm in the lobby area of the Nia Center.
Click here for the Banquet Reservation.


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[View the story "Immigration deal: What's in the Senate proposal?" on Storify]
Immigration deal: What's in the Senate proposal?
Storified by Digital First Media· Mon, Jan 28 2013 06:06:50
A bipartisan group of eight senators will reveal the broad outlines of a proposal to reform U.S. immigration laws today. Articles posted on Politico and the New York Times this morning detailed the proposal.
Here's what we know so far:
It would create a pathway to citizenship
Araceli Cortes, an undocumented immigrant, is shown at her home in California in 2012. (AP Photo/Reed Saxon)
The deal includes a pathway to citizenship for the nation's estimated 11 million undocumented immigrants. Long opposed as "amnesty" by conservatives, this is considered the hardest but most important part of any comprehensive package.
But it would not be easy.
Undocumented immigrants would be required to undergo multiple background checks, pay a fine and back taxes, learn English and pass a civics test in order to become legal residents. Anyone with a serious criminal record would be deported.
Before they are approved, immigrants would be on a "probationary legal status" that would allow them to live and work in the United States but not qualify for federal benefits.
But first, it calls for stricter border enforcement
(AP Photo/Ross D. Franklin)
The pathway to citizenship would not take effect until border security has been beefed up. That would include more drones and border guards and a supervisory commission made up of governors, community leaders and law enforcement officials from the Southwest.
The proposal also calls for an electronic verification system to allow employers to check if they are hiring undocumented immigrants.
Another section of the proposal also calls for better tracking of people in the United States on visas through an exit system at airports and seaports.
It includes parts of the DREAM Act
Undocumented immigrant Layios Roberto waits outside a legal group's offices in Los Angeles in 2012. (AP Photo/Nick Ut)
The proposal would also incorporate parts of the DREAM Act, a long-stalled measure to allow people brought to the U.S. illegally as children to become citizens.
Last year, the Obama administration decided to allow tens of thousands of people in that situation to defer deportation. This proposal would presumably build off that effort.
And it would overhaul legal immigration
An unidentified man takes the oath of citizenship during a naturalization ceremony in 2011. (AP Photo/Mark Lennihan)
The proposal would overhaul the existing legal immigration system to allow more highly skilled workers in so-called STEM fields of science, technology, engineering and math, in part by giving green cards to immigrants tho obtain advanced degrees in those fields from American universities.
Supporters of an overhaul include many in the business community in areas like Silicon Valley and standalone bills have been successful before. These measures are considered vital to help sell other parts of a comprehensive reform bill to skeptics in Congress.
The bill would also allow more low-skill workers into the country, creating a new program for seasonal agricultural workers.
Elements of the proposal poll well
(AP Photo/Ryan J. Foley)
In recent surveys, 70 percent of Americans supported the DREAM Act provisions and 62 percent supported a pathway to citizenship. Groups lobbying for STEM immigration claim to have broad support as well.
It also has bipartisan support in the Senate
The proposal was crafted by a group of eight senators from both parties which includes former Republican presidential candidate Sen. John McCain of Arizona and high-ranking Democratic Sen. Chuck Schumer of New York. Other members: Democratic Sens. Dick Durbin of Illinois, Michael Bennet of Colorado and Bob Menendez of New Jersey and Republican Sens. Lindsey Graham of South Carolina, Mike Lee of Utah and Jeff Flake of Arizona.
Other Senate Republicans have also sounded positive notes about an immigration bill in recent weeks.
A civil rights group opposes the employment verification.
The American Civil Liberties Union said in a statement that the framework agreed on by the senators could provide important protections for illegal immigrants who are exploited by employers and live in "constant fear" over their immigration status.
But the ACLU took issue with the proposal to require employers to use an electronic employment-verification system, calling it "a thinly disguised national ID requirement" that would undermine employees' privacy and lead to discrimination against those "who look or sound 'foreign.'"
The Associated Press contributed to this report.


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CO-OP Hails Visa-First Data EMV Deal
The path to chip-and-PIN debit cards for U.S. credit unions got wider this week with an agreement between Visa Inc. and First Data to share Visa's common debit solution technology for EMV adoption.
"The common debit solution will both support issuer choice and flexibility, allowing network changes without reissuing cards, and merchant and acquiring routing choices without costly host systems reprogramming. Minimal updates are required for all when new participants adopt the solution," Visa and First Data said in their joint announcement on Wednesday.
A liability shift coming in October 2015 is raising the stakes for card issuers, and CO-OP Financial Services, a participant in Atlanta-based First Data's widely used STAR Network, hailed the news.
Many of CO-OP's clients are STAR participants and CO-OP supports STAR's licensing agreement with Visa, CO-OP said, adding that it, too, is working to provide similar functionality in the marketplace.
The major card brands are moving toward adopting the EMV model, which features an embedded chip that generates a one-time code for each transaction. Widely used internationally, the chips are considered vastly harder to replicate for fraud purposes than the traditional magnetic stripe still in use in the United States.
The license agreement will help enable the development of regulation-compliant debit EMV solutions, the Visa-First Data statement said, that will facilitate different types of debit transactions, including PIN, PIN-less and signature cardholder verification methods.
Adding First Data's STAR Network also will give Visa debit issuers a choice among several competing network options for their EMV chip debit cards, said the joint statement from Visa and First Data. The STAR network comprises more than two million retail point-of-sale and ATM locations.
CO-OP said in its statement Thursday, "Like STAR, CO-OP is among the leading PIN debit networks in the Debit Network Alliance working to provide a structure for the common deployment and implementation of the debit EMV standard. One of the ways this can be accomplished is through agreements with other networks, such as the Visa-STAR agreement."
The Debit Network Alliance was created late last year to work toward a common debit chip solution to maintain an open, competitive debit network marketplace.


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Discover a selection of top forums ~roc~ half on the internet. Come to share your passion on forums ~roc~ half and meet other fans of ~roc~ half. Then, why don't you create your own forum of ~roc~ half ?
An RPG site that is based off of Rick Riordan's Percy Jackson series! Come join Camp Half-Blood and RP, chat, and meet new campers! Have fun and watch out for monsters!
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A place for Percy Jackson and the Olympians fans to roleplay.
percy, jackson, demigod, camp, #half, blood, forumotion, olympus, forum, fanfiction, site
Based off of Percy Jackson and the Olympians
Romanian Team forum; Forumul clanului de half-life si adrenaline gamer mod, Romanian Team
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Magical Gamers: A forum for games, stories and much more!
magical, gamers, roleplay, forum, games, stories, much, more!, video, fallout, vegas, touhou, half-life, roleplaying
A Percy Jackson and the Olympians RPG. Free forum : Half-Blood Hill
#half, blood, hill, percy, jackson, olympians, role, playing, lightning, thief, monsters, titans, curse, battle, labyrinth
Free forum : l o v e i s t h e n a m e i n t h e w i n d
A Half-Life 2 Source Mod set in the jungles of Vietnam
realism, fall, saigon, gaming, half-life, source, engine, moddb, vietnam, jungle, warfare
Demigods have choices to make: Become a hero and please the Olympians, or help the Titans tear Olympus down brick-by-brick: A Percy Jackson RPG
The Crew is a gaming community based in GarrysMod's HL2RP.
crew, diablo258, hl2rp, community, garrysmod, serious, clockwork, work, open, aura, #half, life, role, play


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Not exactly what you'd call a reliable source, but they seem to be convinced!
James White will continue his career in the NBA. The American forward agreed to terms with the New York Knicks and will sign a guaranteed contract with them.
White has played in Italy over the last two seasons with Dinamo Sassari and VL Pesaro.
White was the top scorer in Serie A in 2010-11 with 20.2ppg while in the season 2012-13 he scored 17.1ppg.
The forward has already played in the NBA with Spurs and Rockets averaging 5.6ppg in 10 games.
[Only registered and activated users can see links. ]
Check out the FT Line 2 handed windmill!


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BME103:T130 Group 13
|BME 103 Fall 2012
| Home
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Wiki Editing Help
When we unplugged the PCB Board of the LCD from the Open PCR Circuit Board, the machine's LCD screen turned off.
When we unplugged the white wire that connects the Open PCR Circuit Board to the 16 Tube PCR Block, the machine could not register or measure the temperature. The LCD screen displayed seemingly random numbers including -40 degrees Celsius (which is not possible because the Open PCR machine was not changing the temperature at that time).
On October 18, 2012, our group first tested the Open PCR Machine. At first, the machine seemed overwhelming in its design. However, after following the instructions and advice from peers and professors, we were able to determine how to properly setup, program, and run a simple test.
Polymerase Chain Reaction
Polymerase Chain Reaction is a technology that amplifies a single piece of DNA. This technology works very similarly to the natural DNA replication cycle. One PCR cycle consists of three basic steps, denaturation, annealing and extension. In the denaturation step, heat (usually about 95 degrees Celsius) is used to separate the DNA into two strands. Then in the annealing step, the temperature is decreased to 50 degrees Celsius and the DNA primer, specific to the target sequence for that organism, anneal to the separated strand of DNA. The primers mark the beginning and the end of the targeted DNA sequence. Finally, the extension step required the temperature to be raised to 72 degrees Celsius so that the DNA polymerase is activated. The DNA polymerase begins synthesis at the DNA primer. This results in two double stranded target DNA sequences. The PCR cycle is repeated many times to amplify the targeted strand.
There are typically many cycles that need to take place in the PCR in order to amplify a patient's DNA.
After the DNA has been through the thermal cycler, mix each new DNA sample with the the PCR master mix (Taq DNA polymerase, dNTP's, MgCl2, forward primer, and reverse primer) into 8 different Eppendorf tubes using separate pipettes to reduce contamination (see Table 1).
Flourimeter Measurements
After assembling the fluorimeter, you can now actually see if the target DNA was amplified in this experiment.
Research and Development
Specific Cancer Marker Detection - The Underlying Technology
The reason that the cancer-associated sequence of r17879961 will produce a DNA signal while the non-cancer DNA sequence of the same SNP (single nucleotide polymorphism) will not produce a DNA signal lies in the arrangement of nucleotides at the molecular level. The lack of a DNA signal is due to the inability of the reverse primer to bind to the forward strand during the annealing phase of PCR. To detect the cancer-associated sequence of r17879961, the reverse primer AAC TCT TAC ACT CGA TAC AT is used. This is because the cancer-associated mutation is represented by a single nucleotide in a particular triplet: instead of the normal ATT, the middle T mutates into a C, thus rendering a triplet of ACT (which you can see in the reverse primer shown above). At the protein level, this mutation of 1 nucleotide changes the coded protein from isoleucine to threonine. As a result, the primer will not attach to the normal r17879961 DNA sequence as it will not have the corresponding base pairs (TGA) in the particular section of DNA that the mutated sequence would have.
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)


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COMMERCE CITY, Colo. (Friday, September 21, 2012) – Soccer for Success, a youth development program that uses soccer to combat childhood obesity and promote healthy lifestyles, will celebrate its launch with a special media event next Tuesday, September 25 at 3:30 p.m. at Maxwell Elementary School in northeast Denver. The open practice will showcase the growing health and wellness program available to boys and girls throughout the Children's Corridor.
Soccer for Success is a combined effort of the U.S. Soccer Foundation, America SCORES Denver, The Piton Foundation and the Colorado Rapids Youth Soccer Club, and is currently available at nine elementary schools, with plans to expand to as many as 16 next spring. The schools are all located in the Children's Corridor, a 14-mile stretch from northeast Denver to Green Valley Ranch that is home to 54,000 children, roughly two-thirds of whom face the hardships of poverty, underperforming schools, undereducated parents, poor nutrition, or unsafe neighborhoods.
In addition to students from Maxwell Elementary School participating in their practice from 3:30-5:00 on Tuesday, the event will also feature the presence of coaches, educators, community leaders and Rapids midfielder Pablo Mastroeni.
WHAT: Soccer for Success Denver launch event/open practice
WHO: Youth soccer players and coaches from Maxwell Elementary School
- Maxwell Elementary School Principal Dr. Ben Cooper and Vice Principal Cesar Rivera
- The Piton Foundation CEO Terry Minger
- Colorado Rapids Youth Soccer Club Executive Director Jon Goldin-Dubois
- America SCORES Denver Executive Director Brandon Blew
- Colorado Rapids midfielder Pablo Mastroeni
WHEN: Tuesday, September 25, 3:30 – 5:00 p.m.WHERE: Maxwell Elementary School 14390 E. Bolling Drive Denver, CO, 80239


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if stat walked in on that shoot, theyd be all over his dick
Terrible song, but I agree withe message.
I don't want to see Amare at all in these playoffs. He undoubtedly will hurt our team D. No thanks.
You guys are crazy. having stat in the playoffs is the only way we will even be able to contend against Miami.
Yeah, defense wins championships. Personally I believe Stat can bring better defense depending on the opponents line up. When Stat WANTS to play defense he can be a beast. And being out for so long, I'm sure he does. I'm not saying start Amare because I think he'll burn out if he plays all out offense AND defense for 35+ minutes but if he plays around 20-25 minutes per game I'm sure the Knicks can maximize his talents like that. Sort of how we are using JR Smith. You need inside post presence against the Heat. There is no way else were going to beat them. They're defense is too good and will leave the knicks to depend on their outside shooting. They need to rotate Tyson, Kmart and Stat and dominate the paint to win against the heat. It will open up easier shots for our shooters. Now that we have felton and shump back Stat doesn't have to worry about team defense as much and focus on his man. It'll be easier for him on defense. Melo and JR can benefit off his post presence.
His defense can't be any worse than Novak's and at least he'll get us some points. Hopefully he only gets like 15 minutes against second units.
Yeah, he seems to have been rejuvenated energetically and more polished after practicing with Hakeem Olajuwon. His FG% this year is back up where it should be, close to the .600's where he was when he played for the Suns. Since he got both knees cleaned up and got a good rest, I'm hoping he will be much healthier and energetic.
Wow um um um...had fun showing this to the guys at work....these dudes need to come up with a "Derrick Rose Remix" lol
Stat is an important element in the Knicks possibly winning a championship. I could bet that this series with Boston would have been over if Stat would have been playing. With Stat in the game, it puts pressure on the opponents Bigs Stat would have the difference of Garnett almost fouling out and actually fouling out. Against the Pacers, Chandler and K-Mart, are NOT putting pressure on the West Hibbert, Hansborough, to get them into foul trouble. Stat will. As far as defense goes, Felton has been playing $hitty D all year, he still played tho. Melo D is improved, but his D isn't nothing to write home about. Point is, you can have an all world defender, if your team defense isn't worth ****, then you are in trouble. The TEAM defense needs to be good in order for the Knicks to compete for a chip.
I think that if Mike D'Antoni had Steve Novak driving the lane and kicking the ball out to Lebron James, back three months early from shoulder surgery, for threes, there would be some fools out there saying "The Knicks are better off without Lebron James".
Anyone who thinks we can win a chip without STAT is just straight delusional....
screw amare's D...we need his post offense and ability to draw fouls so he can show Melo and J.R what it means to shoot high percentage shots
"The Knicks are back" - Amare
Woody needs to be rotating Camby in these Pacers game to put D on Hibbert. The knicks are allowing Hibbert to do what he wants. We can't let Hibbert be confident in there. We have to foul him if that's what it takes but make sure he don't get easy buckets.
"Everyone wants a champion's power, but no one wants his pain." - Amar'e Stoudemire


text: http://www.tennis-warehouse.com/Wilson_Shock_Shield_Hybrid_Grip/descpageWILSON-WSSHG.html I've just used this grip on my AG 4D 100 and Biomimetic 200 Lite. It's heavy and in a standard installation it seems to add about .4 oz to a racquet's strung weight but it's worth it the extra weight! Other "comfort" grips feel mushy with a loss of feel and control. With this grip you still feel the bevels, there's excellent feel and control, and yet the comfort is on par with my Volkl's and their special Sensor grip system. If you can take the extra weight and want a really comfy grip with no loss in feel or control then this is the PERFECT comfort grip. Traction is outstanding too and I sweat pretty bad. For those with a one-hand back-hand try this: I cut my replacement grips down to 3/4 length so it fits my hand perfectly. I use a color-coordinated overgrip to cover the remainder of the handle. Looks great, you still have some texture for two-handed volleys, and you shave off some weight.


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OCALA, Fla. - A 20-year-old Ocala mother was arrested on suspicion of taking her two babies to an armed robbery.
Alaina Danielle Johnson was arrested Wednesday on charges of robbery with a firearm.
According to the Marion County Sheriff's Office, Johnson drove Matthew Allison Thomas, 18, and a 17-year-old to a home and she also had three others and her babies in the truck. The ages of the babies were not released.
A robbery took place, and the victims told detectives that Thomas and the teen robbed them of cash, marijuana and a paintball gun while pointing a gun directly at the head of one of the victims, deputies said.
Deputies said Johnson, Thomas and the teen were arrested later Wednesday. All three face charges of robbery with a firearm.
Johnson and Thomas were taken to the Marion County Jail and were each being held on $50,000 bond. The teen was taken to Marion County Juvenile Detention and has no bond.
No other details about the robbery or the babies were released.
Anyone with information about the robbery is urged to call Crime Stoppers of Marion County at 352-368-STOP.
Copyright 2013 by ClickOrlando.com. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


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Now in its fifth year, the RadioShack All-Star Futures Game features the top U.S. and International Minor League prospects competing against each other as part of RadioShack All-Star Sunday. The seven-inning affair will begin at 4:30 p.m. (CDT) and will be televised live on ESPN2.
RadioShack All-Star Sunday is the first of three days of All-Star events at U.S. Cellular Field in Chicago, culminating with the 74th All-Star Game on Tuesday, July 15. The outcome of this year's All-Star Game will have implications for the 2003 postseason, as the League that wins the 74th Mid-Summer Classic will receive home field advantage in the 2003 World Series.
Fisk, the first unanimous winner of the American League Rookie of the Year Award in 1972, played for both the Boston Red Sox and the Chicago White Sox during his 24-year Major League career. He spent the first 11 seasons of his career in Boston, winning a Rawlings Gold Glove in 1972 and earning seven trips to the All-Star Game. In 1975, Fisk helped lead the Red Sox to an American League pennant and hit a dramatic game-winning home run in Game 6 of the World Series against the Cincinnati Reds. As a member of the Red Sox, Fisk became the fifth catcher in Major League history to score and drive in a hundred or more runs in one season (1977). With the White Sox, Fisk was a member of four AL All-Star teams and is currently third on the franchise's all-time home run list with 214.
On July 23, 2000, Fisk became the 13th catcher inducted into the National Baseball Hall of Fame in Cooperstown, N.Y. He was inducted into the Red Sox Hall of Fame on September 8, 1997 and later became the fifth Boston player to have his number (27) retired by the club. His number (72) was also retired by the Chicago White Sox in 1997. Fisk ended his career as the all-time Major League leader among catchers in both games (2,226) and home runs (351 of his career 376). He remains one of only three catchers in Major League history to amass 300 home runs, 1,000 runs and 1,000 RBI.
Pedro "Tony" Oliva Jr., born in Pinar Del Rio, Cuba, spent his entire 24-year Major League career with the Minnesota Twins as both a player and a coach. He was named the 1964 American League Rookie of the Year and is the only player in Major League history to win batting titles in his first two seasons; eventually winning three in his 15-year playing career. Oliva led the American League in hits five times (1964-66, 1969, 1970) and ranks among the Twins' career top ten in virtually every offensive category. As an outfielder, he won a Rawlings Gold Glove in 1966.
Oliva is a member of the Twins Hall of Fame and his number (6) was retired in 1997. He finished his playing career following the 1976 season with a .304 lifetime batting average, 1,917 hits, 220 home runs, and 947 RBI. He currently works as a hitting instructor and scout in the Twins' organization.
The 25-man rosters and coaches for the U.S. and the World Futures Teams, selected by Baseball America in conjunction with Major League Baseball and the 30 Major League Baseball Clubs, will be unveiled live via the MLB All-Star Futures Selection Show on MLB.com Radio on Monday, June 23 at 3:00 p.m. (EDT).


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Tue Oct 22
- CLE Credit
- Download to calendar
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Employment Law Institute
- Eastern Time (US & Canada)
- By: PLI
PLI New York Center
- Employment
Why you should attend
Our annual Employment Law Institute combines a comprehensive review of case law and regulatory developments, an in-depth analysis of emerging issues, and best practices to maximize employment law compliance, mitigate legal risk and achieve business objectives. The faculty is comprised of nationally recognized management and plaintiffs' attorneys, members of the judiciary, in-house counsel and government enforcement agency officials who share their perspectives, insights and experiences.
Register Here.
What you will learn
- The Year in Review: the important federal and state case law and regulatory developments, including the latest guidance on arbitration and class actions
- EEOC and DOL's current focus - hear from the source about current priorities
- Settlement issues in wage and hour class/collective actions
- Recent developments under the ADA/ADAAA, including an all new panel on employer assessments and accommodation of mental disorders
- Managing leaves of absence: FMLA, ADA, Workers' Compensation and other types of leave
- NLRB current controversies and labor law in union and nonunion workplaces
- The virtual workplace: risks and opportunities
- Social media and privacy in the workplace
- Retaliation and whistleblower claims, including insight into the non-retaliation provisions of the Affordable Care Act
- Views from the bench - litigating employment law cases
Who should attend
This program is designed for private practitioners, in-house counsel, and human resources and other business professionals who seek an in-depth analysis and discussion of often subtle and rapidly evolving issues arising in employment law.
PLI Group Discounts
Groups of 4-14 from the same organization, all registering at the same time, for a PLI program scheduled for presentation at the same site, are entitled to receive a group discount. For further discount information, please contact firstname.lastname@example.com or call (800) 260-4PLI.
PLI Can Arrange Group Viewing to Your Firm
Contact the Groupcasts Department via email at firstname.lastname@example.com for more details.
All cancellations received 3 business days prior to the program will be refunded 100%. If you do not cancel within the allotted time period, payment is due in full. You may substitute another individual to attend the program at any time.
- CLE Credit Comments: 14.oo Professional Practice; 1.00 Ethics
- Website: www.pli.edu


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Nice looking pie.
Why did you think it was dangerous?
Just that you have a 700F metal box in the middle of your kitchen. I've accidentally touched 350F pans just out of the oven, and that's no fun.
Bert, how come I can't ever see your exported stuff anymore. I'm feeling left out here a bit and I also knew you were doing something inside the house with MPO! Disgruntled Bob... (one of your original supporters man )
MPO outside enclosure temp was around 190 - 200 F
Bob, what exported stuff? do you mean you can't open youtube link? I always appreciated your input and always will. Don't feel disgrunted.
Here is a link to another test of MPO on my gas stove top, bake time was about 2.5 minute. My stove top grating did get discolored, and the marble around the stove did get hot.
Thanks Hong, I think it was about 20 minutes preheating, I have 4 burners stove, to get the bottom heated I used a cast iron griddle, which was partially by each of the four burners. see below photo for the discoloration.Main, concern I had using it indoor, the stove base got really.this weekend, I am testing the oven using 5 of colman single burners (10,000 Btu each), I want to see how hot MPO gets by using all 5 buners at 50 % capacity (25000 bt)..
What in the world...you gonna hurt yourself Bert! Gotta tip my hat to your ingenuity though man.


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I would like the ID of this plant. It looks very common but I would like its ID. I found this in california, USA.
Possibly Asparagus
Or fennel??
I would agree with Steve, it looks like a species of Asparagus. The small brown scales where the side branches come off from the main stem are frequently quite hard and with a sharp point.
Are there any flowers on it? If it is Asparagus they would have 6 small, white petals followed by small berries, initially green turning orange/red.


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A Syrian man killed a woman with a machete and wounded two others outside a bus station in Germany Sunday before being arrested, NBC News reported.
The 21-year-old asylum seeker, who was known to police, was having an argument with the woman at the central Omnibus station in Reutlingen before attacking her at about 4:30 p.m. The suspect, whose name was not released, wounded another woman and a man as he fled, according to local police.
The woman died of her injuries at the scene, officials said.
The attack comes as Germany remains on edge following a rampage in Munich, where nine people were killed by an 18-year-old gunman at a city shopping center Friday. Days before, an Afghan teen attacked passengers on a train, severely injuring at least three people.


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When we talk about the leading software NLEs for videographers, we're generally speaking of five products (alphabetically): Adobe Premiere Pro, Apple Final Cut Pro, Avid Xpress DV, Pinnacle Liquid Edition, and Sony Pictures Vegas. Just outside that circle are Ulead MediaStudio Pro, which is faster and cheaper than the rest and more usable than most, but arguably not quite as feature-rich; and Canopus EDIUS Pro, which, in its first 2.5 versions, one might say, failed to make its intentions clear.
Canopus announced EDIUS Pro 3 on December 1, and demo'd the product at DV Expo West in Los Angeles the following week. Significantly, the announcement was somewhat buried under a related announcement: EDIUS NX for HDV, a $1,299 HW/SW HDV import and editing solution. EDIUS 3 is the software itself, released on its own with the full-step upgrade the name suggests.
On paper, EDIUS 3 is remarkably impressive, and seems well-equipped to make use of the company's other technology strengths. It supports "real-time, mixed-format" editing of a variety of types of HD and SD input, including DV, HDV, and MPEG-2. It also promises "bidirectional conversion" of HD and SD resolutions, though it's hard to imagine why anyone would want to convert from SD to HD (where do all those pixels come from?), "unrivaled" real-time transcoding, support for Canopus' acclaimed HQ HD codec, EDL import, and many other pro capabilities.
All of which sounds great, and none of which is terribly surprising, given Canopus' powerhouse digital video portfolio, which includes SD/HD editing systems, widely installed boards like DVStorm and DVRaptor, and the rock-solid ProCoder 2.0 transcoding solution.
What's least surprising about EDIUS' integration with systems like EDIUS HD, EDIUS SD, and EDIUS SP/NX for HDV, however, is that there's no chicken-egg confusion here; EDIUS started as the software component of the company's HW/SW editing systems, and its development arc has happened completely in conjunction with the development of those systems. Now, how much Canopus plans to do with it outside those systems remains anyone's guess. There's no question of how powerful it is within a powerful Canopus video solution, but how will it play elsewhere? And how important to Canopus is it that EDIUS compete with the likes of Xpress DV (which Avid has very effectively positioned for markets apart from its vaunted broadcast-level systems), Final Cut Pro, Vegas, Premiere Pro, and Liquid Edition? The DVStorm bundle clearly makes a good head-to-head match for Liquid Edition PRO, in terms of price point and functionality.
But how much of a contender EDIUS 3 will prove from a pure software standpoint depends largely on what Canopus has done to the interface between versions 2 and 3. The best products in the pro software space combine power and usability/accessibility; when Jan Ozer reviewed EDIUS 2 in June 2004 EMedia (pp. 36-38), he found EDIUS well behind the curve in usability. It's certainly understandable that a company focused on creating codecs and processing, encoding, and delivering video that would swamp others' systems wouldn't be as focused as a software company like Adobe or Sony Pictures on, say, docking editing windows, auto-color correction, or simultaneous application of multiple effects to the same clip (without rendering one before moving on to the next).
Canopus' press release announcing EDIUS 3 highlights performance issues: real-time preview and output and native Long GOP MPEG-2 support for HDV editing, plus the impressive credentials of the company's lossless codecs, and the product's compatibility with other Canopus offerings like DVStorm2 and DVRaptor RT2. It also notes streamlined workflow, direct-to-DVD timeline export, a new QuickTitler with roll/crawl support, and added interface customization options—all very exciting stuff. EDIUS is priced at $699 ($149 upgrade for registered EDIUS 2.x users), which puts it just on the high end of the prosumer NLE software class. But I still wonder: is Canopus really in the prosumer NLE software business?


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iTunes USA Gift Card
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Redeem your iTunes Code for music, movies, TV shows, games, apps, books, and more on the iTunes Store, the App Store, the iBooks Store, or the Mac App Store.
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Welcome to Albertsons Pharmacy!
Safety and trust. Those are the feelings you have when you and your family are cared for by an Albertsons pharmacist. Care happens in many ways at Albertsons Pharmacy. Of course your prescriptions are reviewed and filled by our dedicated team of professionals with the time and commitment to carefully assess your medication history and explain each prescription or issue.
We also care about prevention. That's why all Albertsons pharmacists are able to review your immunization history, identify which vaccinations might be indicated to prevent you from getting an infectious disease, and administer that vaccination right here in our stores. And specially trained Diabetes Care Pharmacists in many of our locations offer group classes, private training sessions, and expert clinical care to assist those with diabetes or pre-diabetes in managing and preventing complications. All at no charge.
So, when you visit your Albertsons Pharmacy, you can rest assured that you will receive the best care available. Safety and trust. It means a great deal!
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Need answers about how Health Care Reform, also known as the Affordable Care Act (ACA) will affect you? The Health Insurance Marketplace will help you determine what meets your needs and is a new way to find quality health coverage, whether you have coverage now and want to compare options or if you are currently uninsured. Open enrollment begins October 1, 2013. Go to HealthCare.gov™ for answers.


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Is Your Horse's Water Bucket Half Full or Half Empty?
- Sep 11, 2013
- Intermediate
- Topics:
Researchers developed a test to find out whether horses are optimistic, pessimistic, or somewhere in between.
Photo: Photos.com
After a training session, your horse goes back to his resting area and looks in his water bucket for a drink. The water level is at the halfway mark. So, does your horse think his bucket is half full or half empty?
Yes, even horses can be optimistic or pessimistic, according to Swiss equine behavior scientists who have developed a test to find out whether horses are one or the other, or somewhere in between. In fact, the researchers recently used the test to determine how positive and negative reinforcement affect horses' outlook: Does it put them in an optimistic or pessimistic mood?
They found that while negative reinforcement fosters more negative emotions, the horses actually become more optimistic once negative reinforcement training ends than with positive reinforcement—at least in this particular study, said Sabrina Briefer Freymond, MSc, a researcher at Agroscope-Swiss National Stud in Avenches.
"Animal welfare is commonly linked to physical state and mental state," said Briefer Freymond. "And because animals cannot tell us how they feel, it's really important—and it's a real challenge—to measure emotions." Briefer Freymond presented her work at the 9th Conference of the International Society for Equitation Science (ISES), held July 17-19 at the University of Delaware in Newark.
In her experiment, Briefer Freymond and her fellow researchers used a novel method—called the "judgment bias approach"—to test horses' level of optimism. Scientists have used judgment bias tests successfully with other species, but never before with horses.
The team first trained 12 mares in a paddock to find a lidded bucket placed in one of two locations several yards apart. If the bucket was placed on the left side, it contained food. If it was placed on the right, it was empty. All the mares quickly learned that there was no point in lifting the lid off the bucket on the right side of the paddock because it was sure to be empty.
Once this level of optimism had been achieved, the researchers trained the horses various tasks using either positive or negative reinforcement. With positive reinforcement, horses are trained to perform a task and rewarded, usually via food, upon completing the desired action. With negative reinforcement, horses are given an unpleasant stimulus—such as pressure from a hand, leg, or whip—until they perform the desired action; the unpleasant stimulus is then removed immediately. During the training process the researchers observed the horses' behavior (body position, attitude toward the trainer, and ear position) and monitored their physiological parameters (heart rate variability, skin temperature, and respiration rate). This training process lasted five days.
Once the training was completed, the mares began the second phase of the optimism experiment. In this phase the team placed an additional bucket at a location between the original two. They established three different locations where they might set the bucket between the "reward" spot and the "empty" spot. Their theory was similar to the "glass half full or glass half empty" concept: If the horses were willing to check for food in the in-between locations, they were considered to be more optimistic than those who wouldn't try at all.
The researchers observed the horses to determine which ones hoped for food in the buckets (the optimistic ones) and which did not (the pessimistic ones). The researchers noted that during the training period the mares in the positive reinforcement group showed more positive behavior signs than those in the negative reinforcement group. This suggested that the mares in the positive reinforcement group experienced more positive emotions than those in the negative reinforcement group, Briefer Freymond said.
However (and unexpectedly, said Briefer Freymond), the mares in the negative reinforcement group showed more optimism than the mares in the positive group in phase two of the optimism test, "despite experiencing negative emotions during the (training)," said Briefer Freymond.
"This was really contrary to our hypothesis," she said. "It could be that since the negative reinforcement group wasn't rewarded during the training part, these horses were more motivated during the (optimism) test. But it could also be that the end of the negative reinforcement training does, in fact, make horses more optimistic."
Similar studies have shown that after intense stress sheep are actually more optimistic than those that have not recently undergone intense stress, she said.
"However, this could also be a phenomenon unique to mares," Briefer Freymond added. "Prior research has shown that it is characteristic of females (of other species) after short- or long-term stress to develop an optimistic bias, but this does not seem to be the case for males."
Further studies using optimism tests might be more reliable if food is not used in the test, she said; a social reward (finding other horses) could be a good replacement for food in a similar test. The Swiss National Stud group's research is ongoing.
About the Author
Christa Lesté-Lasserre, MA
Christa Lesté-Lasserre is a freelance writer based in France. A native of Dallas, Texas, Lesté-Lasserre grew up riding Quarter Horses, Appaloosas, and Shetland Ponies. She holds a master's degree in English, specializing in creative writing, from the University of Mississippi in Oxford and earned a bachelor's in journalism and creative writing with a minor in sciences from Baylor University in Waco, Texas. She currently keeps her two Trakehners at home near Paris. Follow Lesté-Lasserre on Twitter @christalestelas.
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4over CEO Zarik Mergerdichian Provides Business Update
Published on June 27, 2016
4over CEO Zarik Mergerdichian talks with Senior Editor Cary Sherburne about the company's expansion to better serve its customers. He outlines the benefits of outsourcing some work to improve service levels, build new offerings and benefit from 500 line screen printing. The company now has 17 locations across the US and Canada.
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Published: January 19, 2018
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SGIA and PIA: Why the partnership?
Published: January 18, 2018
Ford Bowers, President & CEO of SGIA, talked with us about the partnership between SGIA and PIA, and the value to their respective memberships. A key element is introducing SGIA's membership to the many conferences that PIA hosts, as well as participation in legislative affairs activities.
iLearning from Printing Industries of America: All new, available 24/7
Published: January 17, 2018
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Demonta Blunt did a little bit of everything for Trinity Catholic last year.
Demonta Blunt did a little bit of everything for Trinity Catholic last year.
A fullback by trade, the versatile athlete was much more than that to his Celtic teammates. The senior led the regional finalists in rushing with 828 yards, averaging better than 6.6 yards per carry, and also ranked second on the team with 19 receptions as he racked up nearly 1,000 yards from scrimmage.
But it may have been the 5-foot-9, 170-pounder's bone-crushing lead blocks and overall football IQ that most impressed the Celtic coaching staff.
Celtic defensive coordinator Justin Wentworth watched Blunt hammer his defenders in practice during his career at Trinity, and consistently used the battering ram back as a motivator for his troops.
"Demonta Blunt has been a great player in every fashion of the game for us at TC," Wentworth said. "He never backed down from any challenge and literally had a no-quit attitude. We need more like him."
That balance of power and finesse ultimately landed him an opportunity to play college football when he signed with Southeastern University in Lakeland earlier this month.
"I like what they're doing down there," Blunt said of the start-up program. "It's a great school and kind of a homey environment. I really enjoyed it when I visited. They're recruiting a lot of good players."
Blunt will take his hard-nosed brand of football to an upstart NAIA program that will play its first official football season in 2014. In the meantime, Blunt will do what he's always done: work hard and be ready when his number is called.
"I've been working out and running (this summer)," Blunt said. "I'm working on my speed and agility, because I'll be moving to tailback. I'm trying to get stronger in my lower body.
"I just want to play ball," he added. "I try to give every play 100 percent and leave it all on the field."
The inaugural signing class for the Fire football team includes more than 30 high school seniors stretching from Arizona to Ohio. The group will practice during the fall of 2013 in preparation for 2014 season.
Instead of focusing on the downside of a football season without any actual games, Blunt prefers to look at the additional practice time as a positive.
"We're just looking forward to it as an opportunity to get better," he said of his first year on campus. "It gives us a lot more time to create unity on the team and get better. We'll have to try to practice how we're gonna play next year."


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NCUA MBS Suits Score Appeal Win
The NCUA won a big legal victory Aug. 27 when the U.S. 10th Circuit Court of Appeals in Denver ruled the regulator can proceed with its claims against Wall Street firms over mortgage backed securities sold to the failed U.S. Central Federal Credit Union.
The appeals court affirmed the ruling of the Federal District Court in Kansas last year that a federal extender statute, which allowed NCUA more time to file its lawsuits, applied in the cases of the agency's securities law claims. The defendants in the suits—RBS Securities, Wachovia and nine other defendants who sold 29 MBS to U.S. Central—had argued the agency had filed its claims too late and asked the court to dismiss them.
The bankers claimed that the NCUA was required to file the suits within a three-year statute of limitations they said began when U.S. Central first purchased the investments. The NCUA countered that the clock started ticking when it placed U.S. Central into conservatorship in March 2009.
The lawsuits, which were consolidated by the court, alleged violations of federal and state securities laws and misrepresentations in the sale of the securities to U.S. Central. The court granted part of the defendants' motions to dismiss the case in July 2012 but denied the remainder of the charges, which the banker defendants then appealed.
On April 29, Judge John W. Lungstrum delayed proceedings on the suits pending the appeals court ruling, citing concerns about the time and expense of litigation.
"We're pleased with the Court's decision," said NCUA Chairman Debbie Matz. "We will continue to pursue our claims against firms that sold faulty mortgage-backed securities to corporate credit unions. As liquidating agent for the corporate credit unions, NCUA has a duty to maximize recoveries from responsible parties in order to limit losses to federally insured credit unions."
The decision could breathe life back into a similar case in U.S. District Court in California, which on July 12 dismissed the NCUA's claims against Goldman Sachs over MBS sold to the failed Western Corporate Federal Credit Union.
In that case, Judge George Wu agreed with Goldman attorneys that the NCUA's claims were time barred and the claims should have been filed within three years of the purchase of the investments.
However, Wu—who also presided over the NCUA's suits against WesCorp executives—approved the NCUA's request for an appeal on the statute of limitations issue, saying the issue has generated a substantial degree of disagreement and was material to the case.
To date, the agency has reached $335 million in settlements with Bank of America, Citigroup, Deutsche Bank Securities and HSBC. Those funds, less legal fees, will be applied toward corporate stabilization costs and would reduce any future assessments federally insured credit unions would pay.


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A curvaceous contoured promotional pen that's among the most popular in the industry!
Made of 600d Poly Canvas provides this custom tote bag with a solid structure to transport all your gear and company logo.
These soft puzzle cubes not only are fun to put together but also doubles as a desktop custom calendar and pen holder.
Score a promotional touchdown with this small inflated promotional football.
This cotton terry custom rally towel is the perfect way to market your brand to an active/athletic audience.
Bring a sense of nostalgia to your next event with these Crazy Putty Promotional Eggs. Recipients of every age will love playing with this fun colored putty!
Print logos, slogans, lines, or anything you want on this popular size custom Post-it Notes note pad.
Show everyone who's #1 with this promotional foam finger that fits like a glove.
Show everyone who's #1 with this promotional foam finger that fits like a glove.
This AdPunch Biodegradable Logo Ball is fun for all ages and made from 100% natural, biodegradable latex.
Show your playful side while promoting your company with these Goofy Grin logo highlighters that stand on their own two feet.
Let this Smiling Tooth Brushing Custom Sand Timer help you keep track of how long you are brushing your teeth. Flip over when you begin to brush and don't stop until the sand runs out!
Everyone will smile when they see their logo on custom toothbrushes.
Mark an achievement or congratulate hard work with these custom ribbons available in 23 colors!
Monthly Academic Year Pocket Custom Planners keep your logo visible month after month starting from July to September.
The Promotional EVA Foam Puzzle Cube is great for school events or use as direct mailers thanks to its flat design.
Erase highlighted areas with a stroke of this custom imprinted highlighter with a special clear erasing solution.
This 6" long custom toothbrushes is ideal for juniors or teens and come in a variety of Hot Colors.
Support the USA with this USA Made Classic Promotional Yo-Yo. A unique way to market your logo or event!
A healthy twist on the promotional pen.
A die-cut book shaped custom imprinted bookmark made of soft and squishy EVA foam.
A die-cut circular branded bookmark that will sure put a smile on readers' faces. Made of soft EVA foam.
A feel good promo bookmark shaped like a teddy bear and made of soft and squishy EVA foam.
A die-cut rectangular promo bookmark made of soft and squishy EVA foam.
The Color Combo Custom Toothbrushes are available in a wide variety of colors with bright bristle color combinations.
Bolster those young, creative minds while promoting your public school with promotional products from ePromos. Promotional coloring books are a fun way for kids to learn valuable lessons and let others know that your school promotes life lessons to keep kids safe. Custom imprinted magnets are large enough to display names and contacts, perfect for parents in case of emergencies.


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News & Events
For the Media
Narayanan Neithalath Receives Tenure & Promotion to Associate Professor at Clarkson University
[A photograph for media use is available at http://www.clarkson.edu/news/photos/neithalath.jpg]
Clarkson University President Tony Collins has announced that Narayanan Neithalath has been granted tenure and promoted from assistant professor to associate professor of civil & environmental engineering in the Wallace H. Coulter School of Engineering.
Neithalath has been a faculty member at Clarkson University since fall of 2005. He obtained his Ph.D. from Purdue University, specializing in cement-based materials. His research expertise is in the development, characterization, performance evaluation, and modeling of cement-based materials.
His research projects primarily focus on developing and understanding the behavior of sustainable construction materials for the built environment. His group at Clarkson is actively pursuing research on understanding the material design-structure-property relationships for pervious concrete pavement systems used for runoff reduction and highway noise abatement, developing environmentally friendly concretes that do not contain any portland cement, novel sensing methods for concrete property detection, and the use of several industrial waste/by-product materials in high-performance concretes.
Neithalath was awarded a Portland Cement Association fellowship for his work with pervious concrete systems. A concrete material developed by his group at Clarkson containing waste recycled glass powder as a partial cement replacement material is being used in the construction of the University's new student center.
Neithalath has published more than 35 papers in refereed journal publications and over 50 in conference and symposia proceedings. Two of his publications have received the best paper awards from International Society of Concrete Pavements and Indian Society for Construction Materials and Structures. He is a member of the editorial board for the journal Cement and Concrete Composites (an Elsevier journal) and an associate editor for the ASCE Journal of Materials in Civil Engineering.
He has secured external research funding totaling about $1.5 million from agencies such as the National Science Foundation (NSF), New York State Energy Research and Development Authority, New York State Department of Economic Development, and several other funding agencies and industries.
Neithalath was the recipient of the NSF CAREER award in 2008.
Clarkson University launches leaders into the global economy. One in six alumni already leads as a CEO, VP or equivalent senior executive of a company. Located just outside the Adirondack Park in Potsdam, N.Y., Clarkson is a nationally recognized research university for undergraduates with select graduate programs in signature areas of academic excellence directed toward the world's pressing issues. Through 50 rigorous programs of study in engineering, business, arts, sciences and health sciences, the entire learning-living community spans boundaries across disciplines, nations and cultures to build powers of observation, challenge the status quo, and connect discovery and engineering innovation with enterprise.


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Complete Array List
Browse by Epigenetics
NOTE: To access content of RT² Profiler PCR Arrays (Prior to May 25, 2012), please click here. Please NOTE: The RT² Profiler PCR Array System was upgraded to Version 4.0 on May 25, 2012. This system has replaced the Version 3.0 system with regards to content and assay design, with the Version 3.0 products set for discontinuation effective December 1, 2012.
The RT² Profiler PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
96-well Plate, 384-well (4 × 96) Plate, and 100-well Disc formats are available.Available for cells, tissues, FFPE samples and small samples
To access content of RT² Profiler PCR Arrays (Prior to March 1, 2012), please click here.
Protocol Guide
Cell Fate Commitment: Dkk1, Hes1, Hes5, Jag1, Nodal, Notch1, Notch3, Pax6, Runx2, Sox9.
Developmental Processes:
Embryonic Development: Dkk1, Edn1, Efnb1, Foxc1, Foxd3, Foxf1, Hes1, Hes7, Myf5, Nodal, Notch1, Pbx1, Pcdh8, Pdgfra, Pdgfrb, Rbpj, Tcf15, Vegfa, Wnt4, Wnt5a.
Muscle Development: Edn1, Egr3, Foxc1, Hbegf (Dtr), Hey2, Igfbp3, Jag1, Myf5, Notch1, Pdgfb, Pdgfrb, Tnc.
Epidermis Development: Jag1, Krt14, Notch1, Ptgs2 (Cox2), Snai1 (Snail), Sox9, Tcf15.
Immune System Development: Id2, Jag1, Jun, Kitl, Pbx1, Runx1 (Aml1), Sgpl1, Vegfa.
Nervous System Development: Bdnf, Ccnd2, Dkk1, Dtx1, Efna1, Efnb1, Egr3, Fabp7, Foxc1, Hes1, Hes5, Hey1, Hey2, Heyl, Id3, Id4, Jag1, Kalrn, Nes, Nodal, Notch1, Notch3, Pax6, Pbx1, Rbpj, Rnd1, Vegfa.
Vasculature Development: Edn1, Efna1, Flt1, Foxc1, Foxf1, Hey2, Id1, Jag1, Jun, Nodal, Notch1, Rbpj, Runx1 (Aml1), Sgpl1, Vegfa.
Kidney Development: Adamts1, Bdnf, Foxc1, Pbx1, Pdgfrb, Sgpl1.
Other Development: Nrarp, S1pr3.
Signal Transduction:
Notch Pathway: Crebbp (Cbp), Dtx1, Foxc1, Hes1, Hes7, Hey1, Hey2, Heyl, Jag1, Lfng, Notch1, Notch3, Rbpj, Rbpjl, Snw1 (Skiip).
WNT Pathway: Ccnd1, Dkk1, Frzb (Frp-3), Fzd5, Wisp1, Wnt4, Wnt5a, Wnt6.
Apoptosis: Abl1, Bdnf, Foxc1, Gadd45b, Id3, Igfbp3, Jun, Kalrn, Notch1, Ptcra, Ptgs2 (Cox2), Sgpl1, Socs3, Sox9, Vegfa.
Cytokines: Cx3cl1, Cxcl12 (Sdf1), Cxcl1, Il33, Nampt.
Transcription Factors: Cbfa2t3, Clock, Crebbp (Cbp), Egr3, Foxc1, Foxd3, Foxf1, Hey1, Hey2, Heyl, Jun, Msc, Myf5, Notch1, Pax6, Pbx1, Rbpj, Rbpjl, Runx1 (Aml1), Runx2, Sox9, Tcf15.
Other Genes: Fjx1, Gpsm2, Hspb8, Mark1, Rhov, Tec.
"If you have access to a real-time PCR instrument, you can use PCR Arrays"
RT² Profiler™ PCR Arrays are the most reliable and sensitive gene expression
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transduction pathways, biological process or disease related gene networks. The PCR Arrays can be
used for research on cancer, immunology, stem cells,
toxicology, biomarker discovery and validation, and phenotypic analysis of
cells and transgenic animals. See the complete list of PCR Arrays.
You can use any 96-well or 384-well real-time PCR instrument. See
instrument-specific setup instructions. Click below for detailed
How it Works
The PCR array is a set of optimized real-time PCR primer assays on 96-well or
384-well plates for pathway or disease focused genes as well as appropriate RNA
quality controls. The PCR array performs gene expression analysis with real-time PCR
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Layout and Controls: The PCR Arrays are available in both 96- and
384-well plates and are used to monitor the expression of 84 genes related to a
disease state or pathway plus five housekeeping genes. Controls
are also included on each array for genomic DNA contamination, RNA quality, and
general PCR performance
You can easily perform a PCR Array experiment in your own laboratory, or send
your samples to us and take advantage of our PCR
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*: when using complete PCR array system.
Performance Data
The complete PCR Array System yields a greater-than 85 percent present call with
as little as 25 ng as much as 5 µg of total RNA from a pathway representing
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The complete PCR Array System, with high quality input RNA, is guaranteed to
yield single bands of the predicted size without primer dimers or other
secondary products thus providing the most accurate real-time PCR results
Application Data
Template cDNAs prepared from total RNA of normal human breast and two human
breast tumors (BioChain Institute, Inc., 5.0 µg) were characterized in
technical triplicates using the Human Cancer PathwayFinder™ PCR Array and the Human Extracellular Matrix & Adhesion Molecule PCR Array with the RT² SYBR Green / Fluorescein PCR master mix on the
Bio-Rad iCycler®.
Toxicology Research:
Rezulin (Troglitazone or "Tro" or "T"), a glitazone
PPAR-gamma agonist, was approved for treatment of type 2 diabetes mellitus, but was
withdrawn from the market due to idiosyncratic liver toxicity. Two similar
drugs, Avandia (Rosiglitazone or "Rosi" or "R") and Actos (Pioglitazone
or "Pio" or "P"), are considered to be safe treatments for
the same condition. The expression profile of key drug metabolism genes should
be different in cells treated with Rezulin versus those treated with Avandia and
Hepatocellular carcinoma HepG2 cells were treated at 80% cell confluence with
these three drugs (100 µM, Cayman Chemical) or a DMSO vehicle control for 24 h.
RNA isolated using the ArrayGrade™ Total RNA Isolation Kit was used to
characterize gene expression with the Human Drug Metabolism and
Stress & Toxicity PathwayFinder™ RT² Profiler™ PCR Arrays and
RT² SYBR Green / Fluorescein PCR master mix on the Bio-Rad iCycler®.
Back to Top
PCR Array Application
Examples: Toxicology, Oncology, and Immunology Research
PCR Array Instrument Setup
Instructions & Protocol Files
Primer Assay Brochure
SYBR Green Master Mix Brochure
The complete RT² Profiler™ PCR Array System insures that you will get good results from your pathway-focused gene expression analysis experiment the first time, and every time guaranteed.
The Complete RT² Profiler™ PCR Array System Includes:
Our convenient and high-quality real-time RT-PCR accessory products complete
the RT² Profiler™ PCR Array System and the RT² PCR Primer
Assays. The other components needed for real-time PCR analysis are SYBR Green
PCR master mix, first strand synthesis kit, and an optional RNA QC array.
Other recommended accessory products
NEW: RT² RNA QC PCR Array
Tests for RNA Quality and Inhibitors of RT-PCR Analyses, optional