text:
Mesoporous calcium sulfate-based bone cements (m-CSBC) were prepared by introducing mesoporous magnesium–calcium silicate (m-MCS) with specific surface area (410.9 m² g−1) and pore volume (0.8 cm³ g−1) into calcium sulfate hemihydrate (CSH). The setting time of the m-CSBC was longer with the increase of m-MCS content while compressive strength decreased. The degradation ratio of m-CSBC increased from 48.6 w% to 63.5 w% with an increase of m-MCS content after soaking in Tris–HCl solution for 84 days. Moreover, the m-CSBC containing m-MCS showed the ability to neutralize the acidic degradation products of calcium sulfate and prevent the pH from dropping. The apatite could be induced on m-CSBC surfaces after soaking in SBF for 7 days, indicating good bioactivity. The effects of the m-CSBC on vitamin D3 sustained release behaviours were investigated. It was found that the cumulative release ratio of vitamin D3 from the m-CSBC significantly increased with the increase of m-MCS content after soaking in PBS (pH = 7.4) for 25 days. The m-CSBC markedly improved the cell-positive responses, including the attachment, proliferation and differentiation of MC3T3-E1 cells, suggesting good cytocompatibility. Briefly, m-CSBC with good bioactivity, degradability and cytocompatibility might be an excellent biocement for bone regeneration.
1. Introduction
Calcium sulfate-based bone cement (CSBC) has been widely applied in bone reconstruction owing to its excellent biocompatibility and resorption in vivo. However, CSBC cannot form chemical bonds between the calcium sulfate grafts and bone tissue because of its poor bioactivity [1,2]. In addition, previous studies pointed out that the degradation products (such as sulfate radical ions) of implanted CSBC would decrease the pH and produce an acidic microenvironment, which might lead to an inflammatory reaction with host tissues in vivo [3].
Mesoporous magnesium–calcium silicate (m-MCS)-based biomaterials with excellent bioactivity have emerged in recent years, which have stimulated the growth of osteoblasts and promoted osteointegration [4]. These characteristics made them one of the most intriguing materials in the biomedical field. The bredigite (Ca7MgSi4O16) with apatite layer induced in simulated body fluid (SBF) could support cell attachment and stimulate cell proliferation and differentiation [5]. It was reported that some ions (such as calcium, magnesium and silicon) released from these bioceramics could change the biological environment in vivo and promote bone reconstruction [6].
The magnesium (Mg) ion is a small but important component of bone tissue: deficiency of Mg ions seems to be a risk factor for osteoporosis in humans [7], and Mg ions were reported to be associated with the mineralization of calcified tissues and could influence mineral metabolism through the activation of alkaline phosphatase (ALP) [8,9]. The degradation products of magnesium-containing materials were not only non-toxic but were also involved in various biological processes such as cellular processes, metabolism of vitamin D and enhanced osteogenic gene expression [10]. In addition, Feng et al. pointed out that silicates would neutralize the acidic degradation by-products and stabilize the pH of the surrounding environment [11]. Zhu et al. [12] mentioned that calcium and silicon ions could significantly induce the formation of a bone-like apatite layer.
Mesoporous materials with high specific surface areas/volumes could adsorb a large amount of drugs into their mesoporous structures and diffuse over time in a controlled manner [13]. Lu et al. [4] revealed that the ordered mesoporous magnesium–calcium silicate (om-MCS) would promote new bone formation by inducing apatite deposited on its surfaces. Zhang et al. [14] also explored that ceria (CeO2)-incorporated mesoporous calcium silicate materials had apatite-formation ability and sustained drug-delivery behaviours. Zhu et al. [12] figured out that mesoporous strontium-substituted calcium silicate (Sr-CaSiO3) materials had stimulated the proliferation and enhanced ALP activity of MC3T3-E1 cells.
Considering the few reports of m-MCS-based biomaterials used in bone cement and drug delivery for bone generation, in this study, the novel mesoporous calcium sulfate-based cement (m-CSBC) was developed by incorporating m-MCS into calcium sulfate hemihydrate (CSH), and the effects of m-MCS content on the physical–chemical and biological properties of m-CSBC were investigated. It is reasonable that the m-CSBC might have an appropriate setting time, compressive strength, degradability, good bioactivity and cytocompatibility. Furthermore, the mesoporous channels of the m-CSBC allowed it to be used as a drug delivery agent during drug controllable release. Therefore, vitamin D3 was loaded into m-CSBC and its sustainable release behaviours were studied.
2. Material and methods
2.1. Synthesis and characterization of m-MCS powders
m-MCS powders were synthesized by the template method, using triblock copolymer of poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (P123, Sigma Aldrich Chemistry) as porogen; concentrated hydrochloric acid (HCl, 36% v/v) as catalyst; ethyl silicate (TEOS, (C2H5)4SiO4), calcium nitrate tetrahydrate (Ca(NO3)2 · 4H2O) and magnesium nitrate hexahydrate (Mg(NO3)2 · 6H2O) as raw materials. All the chemicals were obtained from Shanghai Lingfeng Chemical Regent co., Ltd.
In brief, P123 (6 g) was dissolved in deionized water (217 ml) until the solution was clarified [15], then HCl (17 ml) was dropped into solution with stirring for 30 min. After that, TEOS (12.9 g), Ca(NO3)2 · 4H2O (7.3 g) and Mg(NO3)2 · 6H2O (7.9 g) were added into the mixed aqueous solution with stirring for 4 h; the temperature was kept at 38°C to form well-dispersed collosol, which was aged at 95°C for 3 days and then dried at 80°C. The m-MCS powders were obtained by sintering the dried powders at 200°C for 4 h and then raised to 600°C at a heating rate of 1°C min−1 and kept for 6 h.
The morphology and composition of m-MCS were characterized by transmission electron microscopy (TEM, JEM-2010, JEOL Ltd, Japan), X-ray diffraction (XRD, D/max 2550 VB/PC, Rigaku Co., Japan) and energy dispersive spectrometry (EDS, JEOL-6360LV, Japan). In addition, the pore diameter distribution, surface areas/pore volumes were determined by Brunauer–Emmett–Teller (BET, Tristar 3000, Micromeritics, USA).
2.2. Preparation and characterization of m-CSBC
CSH powders were obtained by sintering calcium sulfate dehydrate (CSD) at 120°C for 8 h, then raised to 160°C at a heating rate of 1°C min−1 and kept for 4 h. The m-CSBC pastes were prepared by mixing CSH and m-MCS (0 w%, 20 w% and 40 w%) powders with a ratio of cement powder to liquid (water) of 1 : 0.85 g ml−1. The setting time of the cements was tested with a Vicat needle as mentioned in a previous study [16].
The cement pastes were injected into the Teflon moulds (Ø 12 × 2 mm). Then the cements were cured at 37°C, 100% relative humidity for 5 days. The compressive strength of solidified specimens (Ø 6 × 6 mm) was measured using a universal testing machine (AG-2000A, Shimadzu Autograph, Shimadzu Co., Ltd, Japan) at a loading rate of 1 mm min−1 [16]. The surface morphology and phase composition of the cement specimens were examined using scanning electron microscopy (SEM↑,JSM-6360LV, JEOL, Japan) and XRD.
2.3. In vitro degradability of m-CSBC in Tris–HCl solution
The degradation of m-CSBC was investigated by monitoring the weight-loss ratio and pH change of the solution after the samples were immersed in Tris–HCl solution (pH = 7.4) for different times (with a ratio of liquid to solid of 20 ml g−1); the Tris–HCl solution was changed once a week. The initial weight (W0) of each sample was recorded. The samples were taken out at different time points (7, 14, 21, 42, 56 and 84 days) from the solution and dried at 80°C for 48 h, and the dried cements were weighed again (Wt). The weight-loss ratio was calculated as
The pH change of the solution after the cements were soaked for different times was measured by a pH monitor (PHS-3C, INESA, Shanghai Instrument).
2.4. Mineralization of m-CSBC in SBF
The assessment of in vitro bioactivity of the cements was carried out in SBFs (pH = 7.4) [17]. The samples (Ø 12 × 2 mm) were immersed in SBF (liquid/cements = 20 ml g−1, 37°C) by shaking. The specimens were collected after soaking in SBF for 7 days, then washed gently with distilled water and dried at 60°C for 24 h. The surface morphology and composition were determined by SEM, EDS and XRD. The change of ion concentration (Ca, Mg, P and Si) in SBF solution after soaking 40 m-CSBC for different times was determined by inductively coupled plasma-atomic emission spectroscopy (ICP-AES, IRIS 1000, Thermo Elemental, USA).
2.5. Sustained release of vitamin D3
The vitamin D3 (0.05 g) was dissolved in 100 ml of ethanol, then the m-MCS powders were added to vitamin D3 solution (m-MCS/vitamin D3 solution: 5 g 50 ml−1) with strong stirring for about 2 h, centrifuged so as to remove the unabsorbed vitamin D3 in the supernatant, and then dried at 37°C for 12 h. The cements loaded with vitamin D3 were prepared by mixing CSH and m-MCS powders containing vitamin D3 in different ratios. Then, the cements were put into a Teflon mould (Φ 12 × 2 mm) and cured at 37°C, 100% relative humidity for 5 days. Using the same method, the CSBC, 20 m-CSBC and 40 m-CSBC containing the same vitamin D3content were prepared.
For sustained release of vitamin D3 from the cements, the standard curve with certain concentrations of vitamin D3 was measured in order to make a regression equation. Then the specimens (CSBC, 20 m-CSBC, 40 m-CSBC) were immersed in PBS (pH = 7.4). The PBS solution (2 ml) was drawn out at each time point (1, 3, 5, 7, 10, 15, 20 and 25 days) and the OD value was tested by enzyme-linked immunosorbent assay (ELISA, SPECTRAmax 384, Molecular Devices, USA). The concentration and release ratio was calculated by regression equation and Origin pro 8.0 software.
2.6. Cell attachment
MC3T3-E1 cells were used to investigate the cytocompatibility of the cements (Ø 12 × 2 mm), which were sterilized with 100% ethanol for 6 h and UV radiated for 1 h; 300 µl of cell suspension was added to the samples at a density of 5 × 103 cells well−1 in 24-well plates, and then incubated at 100% humidity with 5% CO2. At 6, 24 and 72 h, the medium was removed, and the specimens were gently rinsed with PBS three times and fixed with 4% glutaraldehyde for 2 h [18]. The fixative was removed by washing with PBS followed by sequential dehydration in graded ethanol (30, 50, 70, 90 and 100%). The morphology of the cell on the cements was observed by SEM.
2.7. Cell proliferation
MC3T3-E1 proliferation was inspected using the methylthiazol tetrazolium (MTT) test. Briefly, the cells (5 × 103) were seeded on the cements (Ø 12 × 2 mm) and incubated in a humidified atmosphere of 95% air and 5% CO2 for different times. The culture mediums were replaced every 2 days. At 1, 3 and 7 days, 40 µl of a 0.5 mg ml−1 MTT solution (Amresco, Solon, USA) was added to the well plate and incubated at 37°C for 4 h. After 4 h of culture, the medium was removed and 200 µl of dimethyl sulfoxide (DMSO, Sinopharm, Shanghai, China) was added to each well to stop the reaction and completely dissolve the purple formazan product. An aliquot of 100 µl was taken from each well and transferred to a fresh 96-well plate. The absorbance was measured at λ = 590 nm on an ELISA plate reader (ELx800, BIO-TEK). A cell culture plate without materials was used as the blank control. All the results were presented as optical density values minus the absorbance of the blank wells.
2.8. Alkaline phosphatase activity
The ALP activity assay evaluated the effect of the m-CSBC (Ø 12 × 2 mm) on osteogenic differentiation of MC3T3-E1 cells. A total of 2.5 × 104 of cells were seeded on each specimen in 24-well plates, then cultured at 37°C in an atmosphere of 100% humidity with 5% CO2. At each culture point, the medium was removed and samples were washed with PBS three times. Then the cell lysate was obtained by adding 1 ml of 0.2% Nonidet P-40 (NP-40) solution at room temperature. Aliquots of cell lysates were incubated with 50 µl of 1 mg ml−1 p-nitrophenyl phosphate solution (Sigma, USA) at 37°C for 15 min. The conversion of p-nitrophenyl phosphate to p-nitrophenol was stopped by adding 100 µl of 0.1 M sodium hydroxide solution. The ALP activity was normalized by the total intracellular protein contents and absorbance at 405 nm was measured with a spectrophotometer (SPECTR Amax 384, Molecular Devices). The ALP activity of MC3T3-E1 cells cultured on blank culture plates served as control.
2.9. Statistical analysis
A minimum of five samples was tested per group for physical and chemical properties, and six parallel samples per group for the cell experiments. p < 0.05 was considered statistically significant. All quantitative data were analysed with Origin pro 8.0 and expressed as the mean ± standard deviation (M ± s.d.).
3. Results
3.1. Characterization of m-MCS and m-CSBC
From the TEM image (figure 1a), it can be seen that the m-MCS showed ordered mesoporous channels and uniform pore size. From figure 1b, the m-MCS displayed a narrow pore size distribution, and the average pore size was approximately 9 nm. The BET results revealed that the specific surface area of m-MCS was 410.9 m² g−1 and pore volume was 0.8 cm³ g−1. Figure 1c shows the EDS of m-MCS; clearly, the m-MCS consisted of Ca, Mg and Si elements. Figure 1d shows the XRD of the m-MCS, which was amorphous with a broad peak at 2θ = 24°.
The phase compositions of the m-CSBC after being cured for 5 days were characterized by XRD as shown in figure 2. It can be seen from figure 2a that the diffraction peak of CSD appeared at 2θ = 11.6°, 20.7°, 23.3°, 29.1°, 31.1° and 33.3°, indicating that the CSH (CaSO4 · 1/2H2O) had transformed into CSD (CaSO4 · 2H2O) [2]. Correspondingly, the peak of CSD was found in 20 m-CSBC (figure 2b) and 40 m-CSBC (figure 2c), but the peak intensity of CSD significantly decreased with the increase of m-MCS content.
Figure 3 shows the surface morphology of the cements. According to 1 k× magnification images, the surfaces of the cements were dense and rough (figure 3a,c,e), and some m-MCS clusters were observed on the m-CSBC surfaces (figure 3c,e). From higher magnification images, CSBC presented as rod-like microcrystalline structures (figure 3b) while a large amount of amorphous m-MCS was evenly distributed onto the 20 m-CSBC and 40 m-CSBC (figure 3d,f).
3.2. Setting time and compressive strength
The setting time of the cements was prolonged with the addition of m-MCS (CSBC = 5.3, 20 m-CSBC = 8.3 and 40 m-CSBC = 11.5 min) as shown in table 1. In contrast with the setting time, the compressive strength of the cements slightly decreased with the increase of m-MCS content; the compressive strength of CSBC, 20 m-CSBC and 40 m-CSBC was approximately 6.4, 5.8 and 5.5 MPa as shown in table 1.
3.3. Degradability of cements
Figure 4a exhibits weight loss of the cements in Tris–HCl solution. It is found that the weight loss of the cements increased with time. The weight loss of CSBC (48.6 w%) was lower than 20 m-CSBC (59.3 w%) and 40 m-CSBC (63.5 w%) after soaking in Tris–HCl solution for 84 days.
In figure 4b, the pH value of the solution for the CSBC decreased sharply within 14 days (from 7.4 to 6.96), and further decreased to 6.89 after soaking for 84 days, which presented an acidic solution. However, the pH of 20 m-CSBC and 40 m-CSBC showed mild changes (from 7.4 to 7.22, from 7.4 to 7.26) within 14 days, and gradually increased to 7.28 and 7.34 after soaking for 84 days.
3.4. Apatite-formation on cements
Figure 5 shows SEM images of surface morphology for the cements after soaking in SBF for 7 days. The lower magnification shows the ball-like apatite scattered on CSBC surface (figure 5a), while a lot of microspheres aggregates on the m-CSBC surfaces (figure 5c,e). Obviously, the apatite aggregates almost covered the 40 m-CSBC surfaces, which were denser than 20 m-CSBC and CSBC (dependent on the m-MCS content). In the higher magnification, the aggregates were found to be made up of regular microsphere crystalline structures, which were the typical morphology of apatite. The apatite became more and more dense on the m-CSBC surfaces, which were m-MCS content dependent (figure 5b,d,f).
To further determine apatite formation on cement surfaces, XRD scans of the samples after soaking into SBF for 7 days are shown in figure 6a. It was found that the apatite peaks (at approx. 2θ = 25.5°, 32.1°, 42.4° and 49.4°) appeared on all the samples [5], and other peaks appeared at approximately 2θ = 20.8°, 23.5°, 29.2° and 47.9°, which belong to the cements. Clearly, the intensity of the peaks for 40 m-CSBC (figure 6a) and 20 m-CSBC (figure 6b) were significantly higher than CSBC (figure 6c), which was in accord with the SEM images. The EDS of the cements are shown in figure 6b. The results revealed the mole ratio of calcium to phosphorus was approximately 1.66, confirming the formation of the apatite layer on the 40 m-CSBC surface.
Figure 7 reveals changes of Ca, Mg, P and Si ion concentrations in SBF after soaking the 40 m-CSBC for different times (12, 24, 72, 120 and 168 h). Ca ions increased sharply in the first 24 h, then declined slightly and gradually went up after 72 h. P ions kept declining, while Mg and Si ions sustained increases during the whole mineralization process.
3.5. Vitamin D3 sustained released from cements
Figure 8 shows the curve for vitamin D3 sustained release with time. On the first day, the cumulative release ratio of vitamin D3 from CSBC was approximately 7.5 w%, while for 20 m-CSBC it was 6.6 w%, which was slightly higher than 40 m-CSBC of 3.6 w%. After that, however, the cumulative release ratio of vitamin D3 from CSBC was slow, and the m-CSBC displayed a higher cumulative release ratio of vitamin D3 with the increase of m-MCS content. At 25 days, the cumulative release ratio of vitamin D3 from 40 m-CSBC, 20 m-CSBC and CSBC was 41.3 w%, 30.6 w% and 17.5 w%, respectively.
3.6. Cell morphology and proliferation
The cell morphologies on the cement surfaces for different times are illustrated in figure 9. It was found that the osteoblasts stretched out and spread well on the coarse surface of the samples at 6 h (figure 9a–c). At 1 day, the osteoblasts were completely spread and tightly attached to the samples, and exhibited normal osteocyte morphology (figure 9d–f). At 3 days, the osteoblasts formed a confluent layer with close attachment to the surfaces of the samples, as shown in figure 9g–i. The results indicated that all the samples had no negative effects on cell morphology and viability.
The MTT assay was used to investigate cell proliferation; the results from figure 10 show that the OD value for 40 m-CSBC was higher than for 20 m-CSBC and CSBC at day 1, with the culture time extension, the OD value for 40 m-CSBC and 20 m-CSBC significantly higher than CSBC on day 3; meanwhile, the 40 m-CSBC was also higher than 20 m-CSBC. However, on day 7, the cell proliferation on m-CSBC became slower but the OD value was still higher than CSBC, with no obvious differences for m-CSBC between day 3 and day 7. The results suggested that the cements containing m-MCS could promote cell proliferation as compared with CSBC, showing good cytocompatibility.
3.7. Cell differentiation
The ALP activity of MC3T3-E1 cells increased with culture time for all the samples, as shown in figure 11. In general, the cells on m-CSBC presented a higher ALP levels than CSBC (p < 0.05). No obvious differences were found for all the samples on day 7. However, a continual increase could be noted on subsequent days; the ALP of cells cultured on 40 m-CSBC was significantly higher than CSBC on day 10 and day 14; the 20 m-CSBC also presented a higher ALP activity than that of the CSBC on day 14.
4. Discussion
The emergence of newly advanced bioceramics of ordered mesoporous materials displaying good bioactivity and biocompatibility has potential for bone tissue engineering [19]. Previous studies revealed that the too short setting time of CSBC (5 min) was not conducive for injecting cement into bone defects for clinical operations [16,20]. In this study, m-CSBC showed a longer setting time (11 min) compared with CSBC (5 min) so that the cements could be easily handled and injected into irregular bone defect cavities.
Some studies have reported that the acidic degradation products, released by the CSBC, would decrease the pH value of the surrounding environment (produced an acidic microenvironment), which might result in inflammatory responses in vivo [1,2,18,20]. In this study, it was found that there was a gradual decrease of pH in the solution (from 7.40 to 6.89), indicating that acidic products were produced during CSBC degradation. However, the pH of the solution for 20 m-CSBC (from 7.40 to 7.28) and 40 m-CSBC (from 7.40 to 7.34) showed little decrease. The results suggested that the m-CSBC containing m-MCS could prevent the pH from further decreasing in the process of materials degradation. It can be explained that the degradation of the m-MCS in m-CSBC might neutralize the acidic degradation products of CSBC and thus compensated for the decrease in pH.
The degradable material would be gradually replaced by newly formed bone tissue in vivo, so the degradability of biomaterials should be in accord with the new bone growth rate [21,22]. In this study, the degradation ratio of CSBC was 48.6 w% after immersing in the Tris–HCl solution for 84 days, while for 20 m-CSBC and 40 m-CSBC it was 59.3 w% and 63.5 w%, respectively. The fast degradation rate of m-CSBC might be attributed to the large surface area and high pore volume of m-MCS. In addition, the degradation of m-CSBC leads to macroporous structures in the cements, which was favourable for new bone tissue growth in materials and promoted bone regeneration [23].
The apatite layer formation on biomaterials in the biological environment plays an important role in the formation of chemical bonds between material surfaces and bone tissues, as well as bone-relating progress [24,25]. In our research, the m-CSBC could induce apatite deposition within one week, while little apatite was observed on the CSBC surface. The Ca ions from CSBC were not sufficient to promote the formation of new apatite on the material surface, and so it is improbable to generate bone connections with the surrounding tissues because the CSBC lacks bioactivity [18,26–28]. However, the excellent bioactivity of m-CSBC was attributed to the synergistic effects of Ca with Si ion release from m-CSBC during apatite formation (silicon ions are favourable for the mineralization), which could be explained as follows: as m-MCS gradually degrades, the Ca-O bond was broken, more Ca ions released and exchanged with H3O+ in SBF, which facilitates the formation of HSiO43–; the concentration of silanol groups provided more nucleation sites for apatite. Then, the Ca2+, HPO42– and OH− in SBF interacted and finally deposited on the nuclei through a dissolution-deposition process, forming the apatite layer on m-CSBC surfaces [29]. A study revealed that the ordered mesoporous bioglass had a superior ability to induce apatite formation compared with a non-porous structure, resulting from the special mesoporous structure of larger surface area and high pore volume [4]. Therefore, with the significantly improved bioactivity, the m-CSBC was expected to form stronger bone-bonding with the surrounding bone tissue by the apatite layer compared with the traditional calcium sulfate cement.
Vitamin D3 can turn into 1,25-(OH)2-VitD3 (active form of vitamin D3) after transformation in kidney and liver in vivo; vitamin D3 is capable of conditioning the metabolism of Ca and P ions; inducing osteoblast differentiation; and regulating mRNA and protein expression [30]. Animal experiments have proved that 1,25-(OH)2-VitD3 had the ability to treat osteoporosis and repair articular cartilage defects by stimulating osteoblast growth in implants, which is a promising drug to help bone regeneration and accelerate the progress of bone construction [31].
In this study, we loaded vitamin D3 into the cements and investigated the sustained release behaviour of vitamin D3 from the cements in 25 days. On the first day, vitamin D3 exhibited a burst release from CSBC because of a large amount of vitamin D3 exposed on the CSBC surface (easy release). In contrast, m-CSBC presented sustained released of vitamin D3 due to the mesoporous structure. After 5 days, the release of vitamin D3 from CSBC became slow, as most vitamin D3 was wrapped in it, which blocked continued release of vitamin D3 unless from CSBC degradation. In comparison to the increase of m-MCS content, more m-MCS was exposed on the m-CSBC surfaces, so that the cements displayed a fast cumulative release rate of vitamin D3. Furthermore, the degradation rate of m-CSBC was faster than CSBC, thus the accumulated amount of vitamin D3 from m-CSBC was more than CSBC. After 25 days, the cumulative release ratio of 40 m-CSBC, 20 m-CSBC and CSBC was 41.3%, 30.6% and 17.5%, respectively. The results suggested that the mesoporous structure provided a large area for drugs to be loaded, which might be favourable for drug sustained release compared with the CSBC.
The cell responses on the interface of biomaterials surfaces and cells including the morphology, proliferation and differentiation were closely associated with cytocompatibility [32,33]. The results revealed that the surfaces of m-CSBC were beneficial to cell adhesion and growth: at 6 h, the morphology of MC3T3-E1 presented normal phenotype with the synapse extend and spread, indicating that the samples have no negative effect on cell attachment. The cell number increased and closely attached to the substrate at 24 h of culture. On the third day, the cells formed a confluent layer on the m-CSBC surface, showing good cytocompatibility. Moreover, through the MTT assay, it was found that the m-CSBC obviously stimulated the proliferation of MC3T3-E1 cells on days 3 and 7.
ALP is a specific indicator to evaluate osteogenic activity and differentiation ability; its activity was positively correlated with osteoblast differentiation [34]. The result revealed that ALP activity of the cells cultured on the m-CSBC markedly increased compared with CSBC on days 10 and 14. Previous studies have confirmed that the ion dissolution products containing Ca, Mg and Si ions from bioactive glasses/ceramics could stimulate osteoblast proliferation and differentiation [35,36]. Moreover, several studies have shown that the silicon ions could improve the ability of osteoblasts and fibroblasts to produce collagen type I, so that cell differentiation can be promoted [37,38]. In this study, the ions (including Ca, Mg and Si) released from m-CSBC might be crucial for cell responses. Therefore, it can be suggested that the m-CSBC provided a Ca-, Mg- and Si-rich environment, which might be responsible for stimulating cell proliferation and differentiation, showing good cytocompatibility.
5. Conclusion
In this study, m-CSBC was prepared by adding m-MCS to CSH. Our findings revealed that the incorporation of m-MCS into cements prolonged the setting time of the m-CSBC, which was beneficial to improving the injectability of the cements. Meanwhile, the compressive strength slightly decreased. In addition, the addition of m-MCS into the cements improved the degradability and apatite-formation ability of the m-CSBC, which was m-MCS content dependent. Moreover, the m-CSBC containing m-MCS showed the ability to neutralize the acidic degradation by-products released from the cements and prevented the pH from deceasing during degradation. It was found that the cumulative release ratio of vitamin D3 from m-CSBC was significantly higher with the increase of m-MCS content. In the cell culture experiments, our findings showed that the m-CSBC containing m-MCS enhanced the attachment, proliferation and ALP activity of MC3T3-E1 cells. In summary, m-CSBC possessed excellent bioactivity, degradability and cytocompatibility, and might be a new biomaterial for bone tissue regeneration.
All participants gave informed consent prior to data collection. Cell experiments were supported by the Ninth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine.
Authors' contribution
Y.D. carried out the experiments and performed data statistical analysis. J.W. conceived and coordinated the study. S.T., B.Y, Y.Y. and H.L. participated in drafting the manuscript. Y.D and J.W. wrote and revised the paper. J.S. performed the cell experiments and collected related data. All authors gave final approval for the publication.
Competing interests
We declare we have no competing interests.
This study was supported by grants from the National Natural Science Foundation of China (51173041, 31271031), the National Science and Technology Support Plan Projects (no. 2012BAD32B01), the International Cooperation Project of the Ministry of Science and Technology of China (2013DFB50280), the Major International Joint Research Project between China and Korea (81461148033, 31311140253) and the Key Medical Program of Science and Technology Development of Shanghai (no. 12441902802).
The authors thank Zhaoying Wu, Jie Chen and Liang Cai for comments on earlier versions of the manuscript.
- Received August 30, 2015.
- Accepted September 9, 2015.
- © 2015 The Author(s)
Published by the Royal Society. All rights reserved.


text:
Black pornstars
There's so many hot black pornstars in the industry, so putting together this list wasn't easy. Checkout our favorite ebony pornstars here on IFL. There's so many hot black pornstars in the industry, so this is the latest top 10 black pornstar. Watch the video: If you liked our video, comment. The adult film industry has not always been very kind to black female porn stars. Despite this, though, there have emerged plenty of chocolate. Hailing from California, Julie Kay began her career in the adult industry in She gets bffs borderlands 2 in. Check out our favorite ebony pornstars and michelle rodríguez nude us know of any that lund escort think we have missed! I can believe no one has mentioned Codi Bryant. Hailing from Milwaukee, Big cocks porn, Audree Jaymes began her career in the adult industry in at porr gifs age mature woman having sex

subset: commoncrawl


text:
Marketing Genius: Are You Ready to Join Life of Gods?
Bütçe en düşük 5000$ USD
Life of Gods is the maker of "Life of Gods" CDs, the world's most powerful alternative solution product, and the only Psychic Induction CDs. Recently, we want to take our market to the general public, and offer ourselves as the true solution to accomplish what people want with life.
Recently, we have ventured our time and effort into outsourcing the world, in order to distribute our products all around the world. We are prepared to offer a MILLIONAIRE opportunity to marketers who show that they deserve this income. We offer a generous commision rate unseen anywhere else, and as long as you have what it takes, you can receive the maximum income.
Below is a chart of our comission rate:
$1,000 or less: 5%
$1,001 - $2,500: 10%
$2,501 - $5,000: 15%
$5,001 - $7,500: 20%
$7,501 - $10,000: 25%
$10,001 - $12,500: 30%
$12,501 - $15,000: 35%
$15,001 - $17,500: 40%
$17,501 - $20,000: 45%
$20,001 or more: 50%
MUST READ: Anyone who is interested in this project must place a bid below. You will receive a PM with link to a document that you must first read, and application you must fill out. ONLY PROVIDERS WHO SUBMITTED THE APPLICATION AT THE END OF THE DOCUMENT WILL BE CONSIDERED FOR THIS PROJECT.
Good luck bidding
Life of Gods
Bu iş için 91 freelancer ortalamada $48784 teklif veriyor
Dear Sir, we are an India based experienced and pioneer company. We have gone through the information that you have provided. And we would like to offer the best of our services. We can understand what you are trying t Daha Fazla
Hey, Please send me the details. Thanks
Hi, We are an experienced team of online marketers. Pls check pm for details.
Kindly send the information
please see pm thanks,
sure i can but i needs advance payment for setup ur work , let me know if u agree with my condition .
Hmmmm. Interesting.
sir we r the best in the bussiness of web promotion
Send details please
i am interested. please send pm.
please check pm for more details
i'll sell it all around Romania, Italy, Spain, Deutchland, France. i'll build special web sites for this on my own. i run an advertising company wich is rated through the best in my country.
Kindly check P.M.
Please send me the details. Thanks.
Please send details. Thanks
Dear, Yes send me all details and forms. Regards
Waiting for details.
Dear Sir/Madam Thank you for allowing us to bid your project. We are a Professional Web Development Company having experience in Web Design and Development. We also do software development an Daha Fazla
Dear Sir, I have read you r complete requirement . we are working on similar projects like telemarketing solutions and sucessfully implemented for our client. We are a group of developers and having a development e Daha Fazla


text:
I hope that you all saw the front page story in the Washington Post last Friday about seminarians at Mount St. Mary's in Emmitsburg (my alma mater). There was a picture of the Blessed Mother on the front page of the Post! The article itself was fair and positive. It was well done. It briefly paints a picture of life in the seminary in the midst of troubled times in the Church. It presents both the human and spiritual elements of formation for the priesthood. The rector and seminarians, including my buddy, Deacon Dave Wells, did an excellent job. Hopefully, our five seminarians from the Newman Center will shine like these guys when they get their 15 minutes of fame.
Below are excerpts from the article. To view it in full, please click on today's title.
Budding priests in a time of crisis:
Seminarians enter scandal-scarred vocation
...For some seminarians, the abuse crisis only made them want to be priests more.
"It invoked that almost boyhood drive to be a hero," said Matt Rolling, 27, a soft-spoken student from Nebraska. "You want to help the church restore its name. You want to be an example of what the priesthood really represents."
To be a priest, Rolling said, means sacrifice. For him, answering God's call meant abandoning all his careful plans -- a career as a forest ranger, the girlfriend he'd been dating for three years at the University of Nebraska, the prospect of marriage and children.
Even now, he said, there are times when he feels a desire for a wife and family. And, of course, there is the issue of sex.
"It's not like when you become a deacon or priest, the hormones somehow shut off," he said. "There are temptations. There are doubts. How do you deal with that? You try to realize that temptation comes from the devil and salvation comes from God. You pray for that salvation. You build up the spiritual strength to look past the distraction. . . . When I see a girl, I try to think, 'If this were my daughter, how would I feel if someone looked at her that way, if someone mistreated her?' You try to move into that role of a father, which is what you're supposed to be, in a sense, as a priest."
Embracing celibacy at Mount St. Mary's is complicated by the fact that the seminary is housed on the same campus as a college, with a student body that includes plenty of young women.
Strolling through a lush garden dedicated to the Virgin Mary, Dave Wells, one of Rolling's close friends, put it this way: "I don't want to sound like it's the only thing we think about, but, yes, it can be tough."
Midway through the conversation, two girls in tight running clothes jogged by. Wells's eyes, however, remained fixed on a statue of Mary.
"It's good practice for us," he said later, "because in the parishes, we'll be surrounded and ministering to women, too. You may as well get used to it now."
Not everyone, however, can. About 15 percent of the seminarians leave without finishing. In the past year alone, Wells has attended two weddings for former seminarians in his class.
"Some of us are called to be fathers in the natural sense," he said. "Some are called in the spiritual sense."
Hello, GW students! I'm Fr. Greg, the chaplain of the Newman Center . This site is a forum for GW students to ask ANY (appropriate) questions about the Catholic faith, related or unrelated to my posts. All comments have to meet my approval before they are posted. I'm sorry for the approval process and I thank you for your patience and understanding. Thanks, and may you know the peace of Christ!
Wednesday, May 19, 2010
1 comment:
- Matt Shoemaker said...
Father! This article was fantastic! My parents read the article and were really impressed with it as well. It was great to see that the Post can write some really good articles when they put their minds to it. Thanks for linking this!
Subscribe to: Post Comments (Atom)


text:
happy Week 5, start Em or Sit Em is geared toward season-long leagues but can also be used for daily fantasy purposes, this world football predictions tomorrow is one of the worst Sunday-Monday slates weve seen in a long time. Everyone.but whether they can hit that spread is another question. So it's not a question of whether the world football predictions tomorrow Steelers can beat the Bears here; the eye test says that they can.
World football predictions tomorrow
in this world football predictions tomorrow section we will try to form a bet different from what other specialty sites are doing. Money that they do not have and pretend to give it away. We will not give "awards" of thousands of euros,our tips world football predictions tomorrow and predictions are made by professional punters and can bring significant gains. Are you passionate about the sports. The best tips to help you win at sports betting. What we offer? Bet tips Football predictions 365bet. You bet? Bet on football games?
adalberto Mejia) - 7/17 Sunday: Colorado Rockies 115 (Jeff Hoffman vs.) - 7/19 Tuesday: Los Angeles Angels -133 (Edwin Jackson costa rica vs brazil betting tips vs.) jesse Chavez) - 7/18 Monday: Minnesota Twins 100 (Bryan Mitchell vs.) back tomorrow with more winners!
FPL teams GW25 FFGeek contributors show their teams. not only this but we also ensure you get the best odds available by scouting out the best price for our darts predictions. Our tipsters thoroughly research each tournament and dig out the free online future prediction.
USA: World football predictions tomorrow!
m - A breakthrough in soccer predictions. Results and Fixtures Membership Soccer Predictions Live In-Play Double Chance. From as little as 1 per soccer tip. First Half Total Goals Over Total Goals Over/Under Soccer Tips.2017 An Outlook on College Football's Toughest Conference. Alabama played as predicted, world football predictions tomorrow sEC Football Preview. Posted By: Kory Kinslow in SEC Football. The SEC is coming off of one of it's toughest seasons in recent memory. May 01,
mIA (56)) - Damien Williams' injury looked bad, that makes Drake a must-grab and immediate RB2 for next week. Week 13 Waiver Wire football pick em sheets Pickups: RBs. LAST CHANCE : Kenyan Drake, and the early assumption is that he's out for Week 13.
Arsenal Pick - 5/21 Sunday EPL Soccer: Manchester United vs. Tottenham Hotspur Pick - 5/14 Sunday English Premier League: Manchester United vs. Arsenal Pick - 5/7 Thursday EPL Soccer: Manchester United vs. Manchester City Pick - 4/27 Sunday EPL Soccer: Manchester United vs. Burnley Pick.
enjoy the magical views of Marrakech from world football predictions tomorrow rooftop restaurants bars. On the top of every other building in Morocco you can find a beautiful rooftop terrace.but I world football predictions tomorrow am going to go 1-0 Chelsea as I just think United are going to go for it and Chelsea will just make a chance or two before the end of the game.
we'd like to apologize to all of our world wide readers/punters for calling these predictions "soccer" picks, however, all information is for viewers 18. If you think world football predictions tomorrow that you have a gambling problem please visit.uk. Welcome to the m free soccer picks section!best betting tips world football predictions tomorrow for football 1x2, by admin Posted in Uncategorized Comments Off on Archive Vip Tickets By admin Posted in Uncategorized Tagged best bet tips football 1x2, betting football tips 1x2, best betting tips,consider the percentage returns, not the absolute amounts. Of course 1 profit doesn't seem like a lot, world football predictions tomorrow but multiply up the stakes to what you can afford and you'll be making money. Take a look through our example.stakes. Yield. 03-2018. Keep in world football predictions tomorrow mind that betting involves high psychological and financial risks. Profit.
paul Merson is back with another round of his world football predictions tomorrow Premier League predictions - how will he get on? Paul Merson reveals his Super 6 predictions for this weekend. Paul Merson reveals his Super 6 predictions for this weekend.helping you with local tips and world football predictions tomorrow serve you amazing Moroccan breakfast. You will have a local host that will (in my experience)) do absolutely everything for you, including showing you around the neighbourhood,witten has five or more points in five of six games this year as the team's safety valve. He's a lock for similar world football predictions tomorrow production against the Seattle Seahawks,
World football predictions tomorrow
here are the sincere testimonials from some of my faithful members. My tips are extremely truthful and world football predictions tomorrow highly cost-effective. I am amazed by what Daniel Johnson has been capable to achieve at otball. As a fellow mathematician and soccer fan,while we arent a person to give them you might consider providing more world football predictions tomorrow to ones list added with.the Debate world football predictions tomorrow On The Debate.but the Pats prevail. Tune in to Patriots GameDay on WBZ-TV at 11:30am, 2018 CBS Broadcasting Inc. Patriots 21, jets 17 Tune in to Patriots-Jets Sunday afternoon on 98.5 The Sports Hub and WBZ-TV the flagship stations of the New England world football predictions tomorrow Patriots. With Patriots 5th Quarter on myTV38 following the game. Powered by m VIP By viewing our video content, you are accepting the terms of our. Pregame begins on 98.5FM at 10am with Patriots Preview, with full postgame coverage after the game. All Rights Reserved.best football prediction world football predictions tomorrow site, double matches, score predictor, prediction site, soccer tips, fixed match, manipulated matches, fixed ticket, football prediction for tomorrow, today prediction, best football prediction site in the world, fixed matches, best football prediction, prediction tips, best soccer prediction site,
we often give back information to top tipsters all over the globe. We assist them, they assist us. South America, romania,UK, we are here 16 hours out of world football predictions tomorrow 24, we have betting associates in countries like: Portugal, norway, eVERY DAY. France and many more.FIXED CORRECT SCORE OFFER CORRECT (EXACT ) SCORE WITH 5 START We have new offer for Football Correct Score betting tips from first hand who are confirmed and with contract between both clubs and this soccer fixed correct scores are 100 safe and odd is.
all for free! Get yourself one of our free bets to follow our tips risk free! We post all of our footbal tips predictions at 10pm the night before. We offer a wide range Football betting tips and predictions,it is all dependent on how one looks at the situation and the details involved. Players, tackle, which is the better of the two choices. Tags: Football, field, sports,
includes the age of players at the start of the 2016 season. Download » world football predictions tomorrow All cheat sheets are in PDF format.
who was the former Falcons offensive coordinator. The Panthers added key defensive players like Julius Peppers and Mike Adams, carolina world football predictions tomorrow also has a history of playing against 49ers coach Kyle Shanahan, 3 Carolina Panthers @ 49ers. And Luke Kuechly is back and healthy.the talk of the beginning and the end, there was never any more inception world football predictions tomorrow than there is now, but I do not talk of the beginning or the end. 3 I have heard what the talkers were talking,oUR FIXED king bleacher report college football predictions week 10 every game football predictions. 9. Number world football predictions tomorrow of buyers is limited to protect teams, because we have cooperation with strong sources. MATCH is with high reliability Its 100 SURE we guaranteed that, posted:, theyll likely be in the market for another Bortles helper in the 2018 draft, we are leading a way of business where clients cant make any kind of loss.Albania Superliga premier league football octopus prediction Top Lushnja Liege - Antwerp Over 2.5 1.96 1:1 Lose Aberdeen - Rangers Over 2.5 1.92 1:2 WIN Gateshead - Luton Luton -0.75 1.91 0:5 WIN Inter - Chievo Over 2.75 1.84 5:0 WIN Caen - Lyon Over.
latest Football Soccer Results world football predictions tomorrow from the Bolivia Football Leagues only at m Football Livescore Website. Bolivian Football jsk betting tips today Results,


text:
Celebrate JP's Musicians at Annual Music Festival on September 9th
This Saturday 20 local solo musicians and bands who either live or work in Jamaica Plain will take two stages for seven hours at the 7th Annual JP Music Festival at Jamaica Pond's Pinebank Field. The Jamaica Plain Music Festival is a 501(c)3 non-profit organization created by Rick Berlin and Shamus Moynihan in 2011 and this year's festival will be held on Saturday, September 9 from 12 to 7 pm. The festival is free to attend. The festival "assembles a convergence of musical genres, drawing upon the wide-ranging artistic community living in this part of Boston," according to the JP Music Festival website. The festival is a family- and pet-friendly event with a kids music activity area and food trucks.


text:
Day trip to the RSPB reserve at Minsmere in Suffolk today. The weather forecast the best day of the week so we thought we'd make the most of it. It was about 90 mins to get there, but well worth it. Here is a small selection of photographs:
|Black-Headed Gull
|Barnacle Goose
|Pair of Avocets
|Konik Horses
|Flying Avocet
|Common Tern
|Another flying Avocet
|A landing Avocet
This moorhen had landed on the wooden post, had a peck round, scooped up a bit of poo and was about to take off, behaviour we've not witnessed before and can't explain at this moment...I'm sure Packham would know in an instant!
|Feeding Avocet
|Hobby with prey


text:
Does anyone know if 93 and 94 radio's will interchange in the olds cutlass these are both cars with steering wheel controls swapped them now I have no program control only the volume control works when installed in the 93 car .Maybe both 20 something year old have some ailments?
swapping radio's
Posted 17 September 2017 - 07:12 PM
Posted 17 September 2017 - 09:44 PM
'92 5 speed GTP - Mod list _____ '06 Grand Prix SE - Daily Driver
Posted 18 September 2017 - 05:21 PM
The OBD system has nothing to do with the radio. They had the same connectors. It's the addition of a clockspring that would though. 94 has an airbag, 93 didn't. I think they took away buttons from the 88-93 steering wheel controls.
Iirc since they changed the controls (and the oddball obd 1.5) 93-94 don't interchange as far as swrc
2003 Pontiac Grand Am (01/12 - 08/13)
1994 Oldsmobile Cutlass Supreme
2002 Chevrolet Camaro (09/13 - 07/16)
1996 Infiniti G20
1993 Chevrolet Lumina Z34
1989 Buick Regal Limited (06/16 - 01/17)
1994 Chevrolet Cavalier RS
2009 Pontiac G6
1984 Chevrolet Camaro Z28
Posted 05 December 2017 - 05:07 PM
- digitaloutsider likes this
Posted 05 December 2017 - 07:31 PM
Can you explain better what you have swapped?
From how I read it, you have a 1994 Radio and you are trying to put it into a 93 car. It would seem odd to me that they would remove part of the protocol for one year, rather than adding to it, but as the above posts mention, it sounds like they did, and you are only getting the common functions (volume). It would not surorise me, as I added steering wheel controls to my g6, and while the volume works, all other functions do different tasks than what is pictured. For instance, seek down is now mute, and disk change 1-6 is now tune up. Supposedly I can get these programmed in, but I have not tried it.
2015 GMC Terrain | 1998 BMW Z3 Dinan
0 user(s) are reading this topic
0 members, 0 guests, 0 anonymous users


text:
I cannot believe it has been two months since my precious angel was born! It honestly feels like it was yesterday. She has grown so much. She is finding her voice and smiling up a storm. I'm so so excited to see what the next month brings for us!
Thursday, February 18, 2010
Marlee is 2 months old!!
Subscribe to: Post Comments (Atom)


text:
Photoshoot (from around 2015-early 2016?)Posted: January 6, 2017 Filed under: Constellation, Uncategorized | Tags: Cardiff, Dave Mackey photography, Illustration, Osian Grifford, Osian Grifford Illustration, photography 1 Comment
For their coursework some local Commercial Photography students were creating a magazine on local artists (in Cardiff). Dave Mackey took these ones with myself showing some of my then recent work, and some older paintings too :


text:
The Information Commissioner's Office has today hit Carphone Warehouse with a £400K fine after a 2015 hack, which resulted in a major breach of personal data for 3.3 million customers + 1,000 staff and indirectly affected the CPW hosted mobile site for United Kingdom broadband ISP TalkTalk.
The Information Commissioner's Office has, by issuing the large fine to Carphone Warehouse, once again showcased its intent to crack down on firms that hold large amounts of customer data and yet fail to secure their systems from cyber threats.
Andy Norton, director of threat intelligence at Lastline, added: "With a revenue of just over £10bn, Carphone Warehouse could have been fined up to £400m if the ICO had imposed the maximum fine of 4 per cent of revenue under GDPR guidance".
The attack affected more than three million customers and 1,000 employees, with the attackers accessing information such as names, birth dates, addresses and bank details.
"A fine might be significant for Carphone Warehouse, but it doesn't magically provide remediation for those affected by the breach", said Tim Erlin, VP at Tripwire.
The watchdog's investigation found that intruders were able to access the data using valid logjn credentials as a result of the poor security measures, which included an out of date version of the WordPress content management system (CMS).
OnePlus 3 and 3T start receiving OxygenOS 5.0.1 OTA update
LG hasn't published a timeline with the regions that will receive the updated OS, though, but it's bound to be rolled out soon. This comes as a breather for OnePlus 5T owners who had been promised an Oreo update at the time of the product launch.
The records for some Carphone Warehouse employees, including name, phone numbers, postcode and auto registration, were also exposed.
The measures used to identify and destroy historical data were also deemed inadequate.
The ICO considered that the personal data involved would significantly affect individuals' privacy, leaving their data at risk of being misused.
Elizabeth Denham, Information Commissioner, said: "A company as large, well-resourced, and established as Carphone Warehouse, should have been actively assessing its data security systems, and ensuring systems were robust and not vulnerable to such attacks".
Following a detailed investigation, the ICO identified multiple inadequacies in Carphone Warehouse's approach to data security and determined that the company had failed to take adequate steps to protect the personal information.
'It is also a shot across the bow of such companies in the run-up to GDPR.
Moira Donegan Outs Herself As Creator Of 'S**tty Media Men' List
Appreciation for Donegan: HuffPost's Dominique Mosbergen compiled reactions to Donegan's essay from journalists. Donegan also notes that old-school whisper networks "are social alliances, and as such, they're unreliable.
'From 25 May this year, the law is set to get more stringent as the General Data Protection Regulation (GDPR) comes into effect.
Carphone Warehouse said in a statement that it accepts the decision and is "very sorry for any distress or inconvenience" caused.
Since the attack in 2015 we have worked extensively with cyber security experts to improve and upgrade our security systems and processes.
Affected customers and employees were informed at the time.
"That sort of fine would make companies take security - and looking after customer's personal information - more seriously".
It said that as a major "data controller", the Carphone Warehouse should have used systems to comply with "the data protection principles".
At least 25 killed by vehicle bomb in Syria
Some residents blame the Islamic State group for such attacks, although the group has no open presence in the province. It said Syrian air defenses confronted the Israeli attacks, but without giving further details.


text:
As the third-largest school district in Texas and the largest employer in Northwest Harris County, Cypress-Fairbanks Independent School District impacts the lives of far more than the 115,000 students and thousands of teachers, employees and administrators across 88 elementary, middle and high school campuses and special program facilities that study and work within our walls. In fact, we're proud that as a top-rated school district, the work we do within our school system extends beyond the schools and permeates within our neighborhoods and businesses, making the greater Cypress-Fairbanks community a better place to work, live, study and play. The Cypress-Fairbanks community excels because of our CFISD cheerleaders; the individuals and businesses that invest in and support our students and community at large.
Today, we highlight Amy Foster-Muñoz, Goodson Middle School sixth-grade teacher, who was her campus's Spotlight Teacher in the 2016-2017 school year.
How long have you lived/worked in Cypress-Fairbanks?
I have lived here for eight years and worked here for six years.
Why are you so passionate about being a part of the Cypress-Fairbanks community?
Cy-Fair is a wonderful dynamic and welcoming community. This is a place where people of all backgrounds come together to serve the community and each other. The people of Cy-Fair love the area and the community. CFISD in particular continues to be a leader at the state and local levels, working to provide a very wide variety of services to its students and families.
How has Cypress-Fairbanks ISD impacted you, your family or your business?
My family is very involved in CFISD. This is my sixth year as a teacher for CFISD, and I've had the blessings to learn so much from my fellow teachers, DIs, and principals. I have taught theatre, speech, and am now working as a sixth-grade World Cultures teacher. Last year I was honored to represent my campus (Goodson MS) as a Spotlight Teacher. I've received my Horizons certification, and am always awarded a wide variety of professional development opportunities. More than that, I also have two children in elementary in CFISD. I've gotten to see how elementary schools grow children as learners and individuals. I've seen the kinds of opportunities provided for them and how hard the teachers work to see them excel. Finally, my youngest two children attend the Barker ELC, an amazing and warm place that have gone above and beyond to make my children feel welcome and loved. It's been amazing to see CFISD from these three different perspectives and to see how important the district is in the life of my family.
What piece of advice would you like to give to Cypress-Fairbanks ISD students?
Don't fear failure. Fear of failure will hold you back more than any lack of knowledge or skill ever could. We become so caught up in keeping up the appearance of forever being smart, successful and fashionable that we sometimes hesitate to venture into new and unexplored territory. Take a risk, and remember failure is the first necessary step toward success! When you were born you didn't know anything at all! Look at all you can do now, you would never have grown so much and come so far if fear was holding you back.
What's your favorite thing to do, see, eat or experience in our community?
Take advantage of campus activities! Schools host book fairs, festivals, fun runs, fall markets, sporting events, family nights and so much more! I love to see the community and the school come together and spend time enjoying the sense of belonging that is so unique to this community. I love to eat at Tea for Two, Sushi Choo Choo and Skip's Farm.
Interested in getting involved, being featured as an upcoming Cheerleader (or featuring someone who you think makes our district great) or in learning more about how Cypress-Fairbanks Independent School District makes the larger Cypress-Fairbanks community a better place to work, live, study and play for all of us? Visit CFISDSpirit.com to cheer along with our team!


text:
Stay up-to-date with school research news and events and check out what's happening across the rest of the university.
Constant mobile phone use by parents could see baby talk become an "endangered language," ECU communications Professor Lelia Green has warned.
WA poets John Kinsella and Tracy Ryan are preparing to launch half a life of work by ECU School of Arts and Humanities Honorary Professor Glen Phillips.
Margaret Rive Press interview ECU South West Literature and Writing student Tiff Hastie who won the 2018 Margaret River Short Story Competition South West Prize with her story 'The Chopping Block'.
The Digitising Early Childhood International Conference, hosted by Edith Cowan University (ECU), will return to the Novotel Langley Hotel Perth from 9 - 13 July 2018, bringing with it top researchers from around the world.
For the sixth consecutive year Edith Cowan University School of Arts and Humanities is partnering with the Western Australian Secondary School Executives Association (Inc) and Sangora Education Foundation to host the Young Originals Exhibition 2018.
Paradise's Parasite III comes to Edith Cowan University's (ECU) Spectrum Project Space after touring at Shanghai's University of Science and Technology (USST), ARK Gallery and Yinghuang Tea Rooms at USST.
ECU's Spectrum Project Space presents solo exhibition Orientalism & Imperialism: Veiled, Unveiled and Reviled by South African born artist Aasiya Evans.
Many Indigenous students from remote areas overcome unique challenges to attend boarding school, and in doing so, reap clear benefits including employability, access to positive social networks and an improved sense of empowerment according to new ECU research.
A blood test that can detect melanoma in its early stages has been developed by ECU's Melanoma Research Group.
Despite Australian men playing a more active role in their child's birth than previous generations, an anonymous survey of midwifery students from Edith Cowan University (ECU) reveals they are still often treated as second-class citizens.
Are you starting your studies with us mid-year? Don't miss your O'Day and other important Orientation events :) p.s. HOT TIP: make sure you like our…
Are you starting your studies with us mid-year? Don't miss your O'Day and other important Orientation events :) p.s. HOT TIP: make sure you like our…
World-first: Our Melanoma Research Group (MRG) researchers have developed the world's first blood test capable of detecting #melanoma in its early…
Please leave a comment about your rating so we can better understand how we might improve the page.


text:
NEWLIFE Yoga Expo's Lineup of Speakers – Palm Beach: Feb 24-25
Join us in Palm Beach on Feb 24-25 and experience some of the most sought after experts in health, wellness, metaphysics,yoga, meditation and enlightenment. These individuals will be presenting on many amazing subjects all geared to enhance your life!
NEWLIFE Expo will have a vast array of free classes, lectures, performances, and expert panels.
This year's expo will have more to offer than ever before. Don't miss it!
To See the last expo's complete Speaker Schedule Click HERE
Annette Hadley
Meet Annette Hadley - trauma survivor and thriver, nurse, artist, writer, owner of Abundance Art. Choose from a variety of healing books, including the workbook These Ain't Your Mama's Mandalas. Purchase Abundance Art merchandise, sign up for Creativity Coaching programs, grab a freebie pen and treat yourself to "A Bit of Abundance" tiny fine art.
Crap And Creativity: Surviving TraumaLECTURE Saturday 2pm room 2C
In this interactive and creative session, trauma survivor and thriver, artist, writer, storyteller and nurse Annette Hadley will teach you a little about trauma and a LOT about healing through creativity. Soak up the energy of the stories behind her paintings. Come away encouraged and inspired in your own journey towards optimal health and well-being. Materials include a personal "Hidden Unresolved Trauma" assessment, along with a list of healing resources. Ideal as a precursor to These Ain't Your Mama's Mandalas!
These Ain't Your Mama's Mandalas:LECTURE Sunday 2pm room 2C
In this creative and fun session, trauma survivor and thriver, artist, writer, storyteller and nurse Annette Hadley will lead you through calming breathing exercises with a bit of bubbly! She will then teach you how to draw and interpret your own mandalas, step by gentle step using a technique learned in her trauma recovery. Materials include pen, paper and crayons. The workbook These Ain't Your Mama's Mandalas can be purchased as well. You don't want to miss this popular session!
Antoine Chevalier
Holographic MeditationLecture Sunday 12pm Antoine is a world-renowned psychic medium (Japan, France, Peru). He is your unique mediator between you and the spirits of your departed loved ones. He breaks free from the holographic matrix creating the illusion of separation. After two minutes of staring in his eyes, you will be able to SEE the face of your departed loved ones projected right in front of his face. He will share with telepathic communications from them.
Holographic MeditationFREE WORKSHOP Sunday 5pm room 2C Antoine Chevalier is the living proof that we do live in a Holographic Universe. Antoine is a world-renowned psychic medium (Japan, France, Peru). He is your unique mediator between you and the spirits of your departed loved ones. He breaks free from the holographic matrix creating the illusion of separation. After two minutes of staring in his eyes, you will be able to SEE the face of your departed loved ones projected right in front of his face. He will share with telepathic communications from them. Antoine will show you how holographic mediation is done. You will experience a profound shift that will strengthen the connection with your own spirit.
Becca Tebon
Becca is aCertified Holistic, Fitness and Nutrition Lifestyle Strategist.
Dr. Brian Clement
History of LifeLECTURE Saturday 1pm room 2A The history of life spans from the beginning to our current understanding of how people lived, what they consumed and the way that we can all thrive. For more than 60 years, Hippocrates Health Institute, that Brian Clement has directed since 1980, has utilized lifestyle in the conquest of disease and premature aging. Hundreds of thousands have attended their life transformation program and many achieved miraculous results by adapting man's original diet. Clement will reveal the deep knowledge via clinical research how you can achieve complete health and longevity.
Sugar, the Kiss of DeathLECTURE Sunday 1pm room 2A Sugar is the most additive substance known to man. Recent research revealed that all forms are 20 times more addictive then cocaine. This substance feeds viruses, bacteria, mold, yeast, fungus, low and high blood sugar and yes; even cancer! Clement's book Sweet Disease will be presented in an enthralling, revealing and eye opening presentation. Hippocrates Health Institute, the center he has directed since 1980, has researched the effect that sugar has on aging and illness and Clement will reveal the shocking truth about this nemesis to life..
Brenda Cobb
Raw And Living Foods Heal CancerLecture Saturday 3pm Gramercy Park (3rd Fl) I completely healed both breast and cervical cancer naturally without using surgery, chemo or radiation. That was in 1999! Learn how I did it and how I have helped others to heal without toxic drugs by using raw and living foods nutrition, emotional healing, cleansing and detoxification. The body was created to heal itself if you give it what it needs! This works and everyone should know about it!
Healed Cancer HolisticallyLecture Sunday 4pm Gramercy Park (3rd Fl) In 1999 I was diagnosed with breast and cervical cancer. Doctors said I had to do surgery, chemo and radiation or I would die in six months to a year. I didn't do it! Instead I ate organic raw and living foods, detoxified, cleansed and emotionally healed. In six months I was cancer free and that was 18 years ago. Learn the 5 reasons the body develops cancer and other diseases and the 5 most important things to do to heal and stay healthy all your life.
Chris Macklin
What's Going On In The WorldLECTURE Saturday 12pm room 2B
A question many of us ponder throughout our lives is the origin of mankind. How did we get here, where did we come from? Take a look into Creator Gods and Sacred Geometry of Communication. It seems that we are surrounded by forces of negativity at times, what is it that we are experiencing? Why are people in so much fear? Where is it all coming from? And what about our World leaders – how are they all connected? How do Manipulations affect Dowsing & Intuition?What is Time Space Continuum?
Divine Healing ExperienceFREE WORKSHOP Sunday 12pm room 2C
Enter the God space and experience the power of Divine Healing with renowned British healer Christopher Macklin. Prior to the group healing, Christopher will explain how Divine Healing works, how Angelic Beings assist him with Esoteric Operations, how he utilizes Tachyon energy, and how negative energies, earthbound spirits, and manipulations can affect one's health. He will also show you how to keep your vibrational level up, and how to re-energize your food to maximize its nutritional value. All attendees will receive Christopher's powerful healing from the ultimate dimension.
David Young
SATURDAY 7PM-10PM BIRTHDAY BASH CELEBRATION CONCERT WITH SOUTH FLORIDA'S PREMIERE MUSIC GROUP HERE II HERE & DAVID YOUNG WITH DANCING & REFRESHMENTSDavid Young has sold over 1,000,000 CDs of his 59 albums. At least 20,000 healers, wellness centers and hospitals around the world use his music every day which reaches about 300,000 people.
Debra Betesh
HOW GRAND MASTER QI SAVED MY LIFEGrand Master Qi said he could save my life, and I accepted the challenge. With Grand Master Qi's help, I stopped taking prescription medication that adversely affected my body. Chinese medicine was the answer. Grand Master Qi made me feel young again. I am grateful for his treatment and wrote about it in my self-help book, Happy Healing: What Would You Do If It Happened to You.
Donna Sessler
SPIRITUAL GEMOLOGY- CHOOSING, USING and WEARING GEMSTONES & CRYSTALS CONSCIOUSLYLECTURE Sunday 11am room 2C
Donnareyna will pass around & share many of her gemstones with their healing benefits and specific qualities relating to the rainbow colors. She will lead a chakra meditation beginning with the black grounding crystals and completing with the white ascension stones. Instructions will be given for cleansing and purification of crystals & jewelry (heirlooms & antiques) with the 4 elements. A sacred crystal grid will be set up in the room that can be duplicated anywhere at any time.
Dr. Robert Young
Are Your Thoughts Killing YOU?LECTURE Saturday 12pm room 2A
Your thoughts require energy and when energy is being used acidic wastes from those thoughts are produced. If your acidic waste products from your thoughts are not eliminated through the four channels of elimination (urination, defecation, perspiration and respiration) then these poisonous waste products will be stored in the connective and fatty tissues which can lead to first enervation, then irritation, followed by inflammation, induration (solidification leading to plaque and stones), ulceration and finally degeneration (cancer).Learn more about the 7 Stages of Acidosis leading to ALL sickness and disease.
Beliefs Control GeneticsFREE WORKSHOP Sunday 11am room 2B
The pH Miracle of Love, The "placebo effect" is in reality the perception or belief
effect. Your beliefs and those of your care-takers are implanted on the anatomical
elements or the intelligent physical beings that make up your genetics and all living
cells. Our beliefs effect our behaviors! These behaviors require electron energy
at the genetic and cellular level powered by the universal power of love. The scientific
equation is as follows: feelings <=> thoughts <=> love
Erika Cappelman
Natural Solutions for Your Health and HomeLECTURE Saturday 11am room 2A
Using essential oils can be a safe, natural option to protect and maintain your family's health and wellness. Learn about a large selection of natural wellness products for a customizable health and beauty routine that's right for you. I love empowering families to maintain health in a natural way and teach you how to be empowered to use essential oils safely and effectively in your own home and be an advocate for your own health.
Gail Thackray
Cutting Negative Relationship Cords-FREE WORKSHOP SAT 4PM RM 2BFREE WORKSHOP Saturday 4pm room 2B
We create cords with anyone significant in our life. These can be good cords but they can also be negative. A difficult relationship of any nature is a sign of negative cords that have anchored and attached and can make you quite sick. Sometimes good cords can get strangled by negative black cords which cause distrust, jealously, fear and anger in our relationships. Sometimes cords are attached to old negative patterns which cause career problems and financial issues. Cords can hold us to our past and can even be rooted in past lives. Gail is a psychic medium who is able to not only see these cords but dissolve and remove them bringing healing in your relationships and release from the past. Discover cords that are holding you back. Releasing negative cords can cause impressive results. Learn cutting cord techniques healers can do on their clients or you can use on yourself and your loved ones. Gail will perform cutting cords for everyone VIPs will sit in the front rows and will receive an individual cord cutting from Gail.
Calculate Trauma Dates To Heal Yourself & OthersFREE WORKSHOP Saturday 4pm room 2B
Learn to pin point actual dates when an energy block or karmic issue first started for you or your client. Sometimes issues start in our early childhood, sometimes we are not consciously aware of them and some actually start in past lives. These affect us for many lifetimes until we finally absorb the lesson and release the karma. Determining the starting point helps you to understand the origin and receive a permanent release. This workshop is for people already doing healing work as well as total beginners. You will learn to become a medical intuitive and read energy. Learn to feel and sense energy blocks and to read the messages that they hold for your client. Then learn to communicate with healing guides to determine when and how these blocks were first formed. You will be working with a pendulum time line grid and communication cards. For professional psychics and readers this is a tool that your clients will love. All attendees with receive an individual psychic opening attunement to become a medical intuitive and receive messages from spirit.
Guides, Angels & Power AnimalsLECTURE Sunday 2pm room 2A
Angels, guides, power animals & healing spirits. Learn to communicate, to invoke them & ask for assistance. Meet your main guides. Receive tools to communicate with them though objects and signals. Develop your psychic language, get yes/no answers and detailed direction. Travel to the underworld, meet your power animal, and invoke animal medicine for healing and readings. How to call in guides for specific task and the best way to contact guides for healing others.
Dr. Grazyna Pajunen
Breakthrough In Anti-Aging – Natural Cartilage Regeneration And Wrinkle RemovalLECTURE Saturday 1pm room 2C & Sunday 4pm room 2C
No pain or wrinkles without surgeries, medications or injections. Come learn about Liquid BioCell Collagen and Jusuru, a liquid nutraceutical with a patented primary ingredient called Liquid BioCell Collagen. Jusuru is natural with no additives and preservatives. It is 100% bio-available, highly effective, and safe. This is the only source of collagen and hyaluronic acid that our body recognizes and utilizes as our own. Grazyna Pajunen has a Ph.D. and has 25 years of experience in biomedical research at UCLA and FAU.
Here 11 Here
Birthday Concert SaturdaySATURDAY 7PM-10PM
Howard Dean
Chemtrails vs Contrails - Sun 5pmWEATHER MOD.PROGRAMS with EMF & HAARP! WHAT YOU CAN DO TO STAY HEALTHY! Howard's FloridaE- coNetwork.com is specializing on Air/H2O Quality info,events. Focused on "Chemtrails vs Contrails" the "Haze" movie. His goal is to help you understand why you and your families are experiencing unhealthiness, tiredness, mental, emotional Relationship problems.You will be presented with the latest information regarding Eco related health problems and health solutions. Howard will discuss EMF,HAARP & Solutions will be our main focus!
Jill Dahne
Love & RelationshipssLECTURE Saturday & Sunday 3pm room 2B
Listed as the #1 Love Psychic in Ameri- ca, Jill wants to get America back on track with relationships. Most women and men want to have a significant other, but do they know what they are really seeking?_ Online datinga:the pros, the cons and why more people are turning to it. Soul mates: What do you really know about them and do they exist?_Share some of my secrets in finding true love.Why good people find bad matches?_Jill will answer audience questions and give insights to their current relationship.
Dr Joel Wallach
Immortality /Dead Doctors Don't LieLECTURE Saturday 5pm room 2A & Sunday 10am room 2C
Live chronologically to 120 while being biologically 50 years of age! Learn the common and secrets of the top 20 longevity cultures on earth. Learn how to employ high ORAC values, calorie restricted diets the "mighty 90" supplement program and the avoidance of "bad" health practices to add 25 to 50 healthful years to year life! These cultures have one hundred year old pet 250 of their population - Americans have one hundred year old per 10,000 of our population Dr. Wallach will reveal their secrets!!
Josh Brotherton
Marconics - The Human UpgradeMarconics - The Human Upgrade
MARCONICS is a multidimensional Ascension energy healing modality, handed down by Spirit, for the evolution of Humanity – The Human Upgrade. The Marconics Energy Healing Protocols bring the client into alignment with Source energy in preparation for merger with multiple aspects of Higher Self, each introducing a higher vibration into the physical body, in a Jacob's Ladder style Ascension process. Learn about its origins, the differences from other healing modalities and more including information on our upcoming Florida practitioner training classes.
Julie Ryan
Come Get The Answer To Your Question.LECTURE Saturday 6pm room 2B
Psychic and Medical Intuitive Julie Ryan can sense what medical conditions and illnesses a person has and facilitate energetic healings. She can communicate with spirits both alive and dead. Julie can scan animals, access people's past lives, and remove ghosts from homes and other buildings, and tell how close to death someone is. Each week, Julie scans callers on her 'Ask Julie Ryan' podcast which is heard by millions in over 100 countries throughout the world. Julie is a businesswoman, an inventor, an author and a serial entrepreneur. Her surgical device inventions are sold globally and she has founded several companies in five different industries. Julie's Psychic and Medical Intuitive skills are learned.
Kat James
Total Transformation: The True "Extreme Makeover" For Your Health, Your Looks, And Your LifeLECTURE Saturday 4pm room 2A & Sunday 10am room 2B
Twenty-five years ago, award-winning author Kat James saved herself-and has since helped free countless others-from multiple digestive, autoimmune, metabolic, emotional, addictive, skin, and weight disorders after doctors gave up. Today, we see a tidal wave of scientific research in support of her once-controversial methods. calibration, and doctor-verified outcomes in successfully harnessing the incredible power of the master hormone, leptin. Unlock the key to your own extreme natural makeover-without surgery or suffering-with the woman SELF calls "a master of self-transformation." Learn more at www.informedbeauty.com.
Lady Christopher Barrett
Lai Ubberud
Direct Past LivesLECTURE Saturday 10am room 2A Lai explains the repetitive patterns in our lives and also why we have certain phobias, likes or dislikes for things, people or places. She also tells about the principles of reincarnation and she will answer questions about reincarnations. She will also give one past life to as many members of the audience as possible and time allows.Lai European Psychic http://www.laiubberud.com Winner of the Award for the "Best Psychic of 2005" and the "Best Pet Psychic of 2007" from the Houston Press. Member of the "American Association of Psychics"
Laura Norman
Transform Your Life with Holistic ReflexologyLECTURE Sunday 2pm room 2B
Ready to transform your life? Discover, and experience, how to reduce stress, increase your energy, and improve your health with Laura's internationally renowned Holistic Method. Laura will show you how your feet are the gateway to regaining your sense of overall balance and well-being. Laura Norman, considered the leading expert in Reflexology worldwide, author of the best-selling book, 'Feet First: A Guide to Foot Reflexology', and an educator with three masters degrees in Education, offers private sessions and Holistic Reflexology Certification Training Programs in The Berkshires, NYC and South Florida.
Lisa Lumiere
Lori Spagna
5th Dimensional Living: Vibrating & Aligning With The 5th-12th Dimension & BeyondLECTURE Saturday 3pm room 2C Expand Your Consciousness & Actively Participate in Shifting Global Consciousness& Serving The Greater Good of Humanity. Receive Ascension Updates.Learn About The 5th Dimension, The 6th Dimension Through 12th Dimensions & Beyond. Activate Your Dormant DNA and Your Dormant Potential. Hear About Our True Galactic & Star Family Heritage and The Galactic Timeline for Humanity's Awakening.. Align with Your God Self & The Divine Source within YOU! Understand Vibrational Energy Healing, Sacred Activations & Cellular Codes of Awakening for You & Your Animal Companions.
Discover The Root Cause & The CURE of EVERY Disease For You & Your Animals TooLECTURE Sunday 4pm room 2B What Your Doctor and Health experts and Even Nutritionists Don't Know and Can't Tell You.Learn How You Can Heal Yourself, Your Loved Ones and Your Animals including physical, emotional, mental and even 'phantom type' illnesses. Expand Your Knowledge and Wisdom of REAL Health Care & Deepen Your Understanding of Energy, Vibrations, Animal Communication & Telepathy. Lori Spagna is a Best Selling Author, Speaker, Visionary, Lightworker and Starseed
Lourdes Starshower
Lourdes Starshower, Tantra Healer, Sacred Sexuality Coach (www.tantrahealerflorida.com) offers workshops, classes and private sessions in North Palm Beach.
The Crystalline MatrixLECTURE Saturday 12pm room 2C
Join this crystalline sound portal of self transformation with Alchemy Crystal Bowl Master Lupito, Founder of Crystal Tones who will be demonstrating Bowl Master techniques and uses of the Alchemy Crystal Bowls. While playing the Rose, Ruby, Diamond, Gold, and Divine Kryon bowls alchemies we will be activating the light in the liquid crystals of our cells bringing us to a higher state of awareness and bliss for profound healing!
Mark Becker
Why Yogis Are VegetariansOne of the fist Hindu words we learn in yoga is "Ahimsa" which means non-violence. So yogis became vegetarians first for Spiritual reasons and then learned the health effects which made our postures easier to do, our minds clearer making it easier to mediatate and our bodies more flexible. Learn the principles of yoga which many teachers have forgotten. Mark Becker opened the first nonhindu yoga center, Serenity, in NYC in 1975 and has been practicing yoga for almost 50 years
Michelle Alva
GODDESS PANELGoddess Panel Sunday 5pm ROOM 2A
Michelle Alva is a Licensed Physical Therapist and Yoga Therapist, Creator Of The Alva Method® Of Quantum Healing.
Nadine Muhammad
Marconics - The Human UpgradeMarconics - The Human Upgrade
MARCONICS is a multidimensional Ascension energy healing modality, handed down by Spirit, for the evolution of Humanity – The Human Upgrade. The Marconics Energy Healing Protocols bring the client into alignment with Source energy in preparation for merger with multiple aspects of Higher Self, each introducing a higher vibration into the physical body, in a Jacob's Ladder style Ascension process. Learn about its origins, the differences from other healing modalities and more including information on our upcoming Florida practitioner training classes.
Paul Utz
Divine Quantum ConnectionLECTURE Sunday 3pm room 2C
Join with Alchemy Crystal Bowl Master Paul Utz co-founder of Crystal Tones. An exploration of the divine quantum crystalline connection between the universal self and our divine spirit. Explore the unique frequencies of alchemy singing bowls and how they affect the different dimensionalities of our human body, thereby bringing about transformational awareness and quantum healing for truth in our lives. Experience the divinity within you now.
Peter Goodgold
Healing With Hydrogen WaterLECTURE Saturday 3pm Skytop rm The discovery and documentation of the "healing" and "curative" waters of Nordenau, Germany; Tlacote, Mexico; and Hita Tenryosui, Japan waters all contain dissolved hydrogen gas. Molecular Hydrogen has been shown in over 500 studies to have positive effects on 100 different human diseases. The antioxidant properties and antiapoptotic (anti-cell death) activity of molecular hydrogen has finally piqued the interest of the biomedical field. Peter Goodgold, shares the complex healing powers of his "Infinity Hydrogen Water Generator". Attendees receive free gift.
Phillip Wilson
Healing Power Of Far Infrared Light Saunas - Sat 5pm"Absorbable Far Infrared Light" (4 - 14 microns) is a tiny band of light from the sun that is absorbed by our bodies. It was discovered about 35 years ago. A man can absorb this incredible healing energy which pulsates his water molecules, increases his core temperature, and promotes extraordinary health. Phillip Wilson has been exploring the inner worlds, mathematics and communication for 55 years, recently making many discoveries about Far Infrared Healing Light. Phillip owns Momentum98.com health store, in Columbus, OH.
Qi Feilong
Energy Healing And Kungfu DemonstrationLECTURE Saturday 2pm room 2B
World renowned Qigong Master QI Fei- long, who was invited to Harvard Med- ical School to demonstrate Qigong as an alternative therapy, will provide a free demonstration of Qi Energy. Watch how internal healing energy can set newspaper on fire, stop a clock, twist a steel spoon and break chopsticks with a $20 bill! Whether the concern is ADD, self-defense or total body health, you will learn how Qigong holistic therapies can help to restore and maintain total body health.
THE SHIFT: Feel It, See It, Create ItLECTURE Saturday 1pm room 2B
Have you been working to create change, and nothing's changed? Is this the year you want to make a Shift? In this FREE lecture SarahSpiritual teaches you to find the missing piece in your manifestation process. Identify your limiting beliefs and the importance of "feeling" your desired manifestation. Learn ways to restructure your self-talk, adjust for conditioned beliefs, feel the exhilaration of a dream life and create the reality. This lecture will include exercises a handout and a meditation.
Power of the Spoken WordLECTURE Sunday 1pm room 2B
The Energy of your words is constantly creating. Understand the importance of your words and thoughts in the manifestation process. Learn how to align your words with the Universal Frequencies and harness the power of the "I AM". This FREE lecture includes powerful decrees and a handout to empower your life with SarahSpiritual.
Shari Hess
REBUILD YOUR IMMUNE SYSTEM & TAKE BACK THE HEALTH OF YOUR GUTLECTURE Saturday 11am room 2C
There is a new crisis in our food supply that is affecting the health of our guts. Find out what the World Health Organization, The European Union, and California has ruled as being toxic and a known carcinogen which happens to be in 75-80% of our food. If you suffer from any of the following symptoms, the health of your gut could be compromised: chronic fatigue,joint pain,difficulty losing weight,headaches,diarrhea,IBS,brain fog,lack of energy,depression,leaky gut syndrome,acid reflux,constipation, rheumatoid arthritis, diabetes,crohns.
Sharon Lynn Wyeth
Know Your Name; Know the Genius in YouLECTURE Saturday 6pm room 2C
In this fun and exciting program, you will gain the power to access others' personalities and motivations quickly by grasping the gifts and challenges hidden within their names. Plus, when you apply this powerful information, you will make better decisions by knowing yourself better. By giving you the ability to instantly interpret the letters in a name, you'll know what to expect and how to work with people for the benefit of all and will give you the ability to better relate with anyone at any time!
Tammy Adams
Twin Flame/Soulmate: Which Is It?
Stop going in circles, Find love now with Master Intuitive Life Coach & Spiritual Healer, Tammy Adams. She will teach and clear the path for you
Terje Kulu
Goddess Panel Sunday 5pm Rm 2A
Wendy Soderman
Embrace Life: Finding Gifts In AdversityLECTURE Saturday 10am room 2C
Join us for a passionate, multimedia presentation as renowned motivational speaker, Wendy Soderman, shares transformational strategies on how you can face adversity with an "Above the Line" optimistic perspective. After participating in Wendy's "Embrace Life" inspirational talk you will be empowered to face challenges head on and turn them into gifts of wisdom to create your own authentic life journey. Wendy is the Founder of the IDEAL SCHOOL of Leadership and of Breathe Modern Meditation Studio in downtown West Palm Beach, FL. Her life story was featured in a documentary with Robert Downey, Jr. Wendy was a key speaker in "The Secret" motivational series along with Bob Proctor and Rev. Michael Beckwith. Everyone who hears Wendy Soderman speak is empowered to overcome life obstacles and make their dreams a reality.


text:
I believe I addressed this in a moar than adequate manner here
I'd like to say I'm a gonna follow you round n destroy your arguments butt they are ruined before you type them?
I believe I addressed this in a moar than adequate manner here
I'd like to say I'm a gonna follow you round n destroy your arguments butt they are ruined before you type them?
"A nation can survive its fools, and even the ambitious. But it cannot survive treason from within. An enemy at the gates is less formidable, for he is known and carries his banner openly. But the traitor moves amongst those within the gate freely, his sly whispers rustling through all the alleys, heard in the very halls of government itself. For the traitor appears not a traitor; he speaks in accents familiar to his victims, and he wears their face and their arguments, he appeals to the baseness that lies deep in the hearts of all men. He rots the soul of a nation, he works secretly and unknown in the night to undermine the pillars of the city, he infects the body politic so that it can no longer resist. A murder is less to fear"
Cicero Marcus Tullius
I voted "No", but really it's "No... with a caveat".
A representative's responsibility is to their constituents... to a point. I don't have an issue with a block of politicians being obstructionist in theory, I'm just not sure the issues being used right now are it.
Roy L. Fuchs: I'll tell you something. This country is going to the dogs.
You know, it used to be when you bought a politician, that son of a bitch stayed bought.
Letting those who need to cause enough Mischief to make their constituents happy.
These are the same constituents who keep wanting to "Take the country back"... from an elected/Re-elected President. (!)
They lost the original vote on on ACA of Course, and don't have the votes to repeal it now either.
It was challenged in the UNSC as well and passed muster.
So What we have then is an Unprecedented and bizarre attempt to take the budget/country hostage on that basis: a passed Law.
If the Democrats/Prez ever allowed a small minority to be able to change/overturn a law using these tactics, it would be the end of our democracy.
Are other members on the Right willing to shut the country down over abortion or other traditional tough issues?
This is Personal vendetta against a bill called 'Obamacare'. The official Moniker ACA, polls considerably better than the Prez Nickname.
Most people who are polled, don't eve know what's in it, just it's called Obamacare.
Six of One - Obamacare vs. The Affordable Care Act - YouTube
But back to the central OP point. Yes, they can do what they were elected to do, but that doesn't mean even Boehner should humor it. He is doing so for party unity and understanding their position.
Allowing a Clean Resolution (to fund the govt) to come to the floor would end the protest and show those who support it they are Not a majority and cannot change the law through these tactics.
These flat-earthers must be made to understand Obama IS the President.. Again.
I think this will end when the Debt Ceiling issue is conjoined and Democrats make other compromises to the GOP, Not Obamacare.
That saves face for everyone and gives Boehner some chips in the big game.
Thanks for the Thoughtful String/OP.
Who would have thought to look in this section for better discussion?
Last edited by mbig; 10-02-13 at 04:30 PM.
I'm personally sick of not being able to dunk a basketball because of racism.
Absolutely, anyone that believes in limited government, low taxation, responsible fiscal policy & personal liberty and responsibility
is a bad person.
yer right the opposition party shouldn't oppose anything this regime does ever.These flat-earthers must be made to understand Obama IS the President.. Again.
you 47%'ers are all going to end up on SSI, eating your dawg food with plastic sporks and paper plates
then end up in front of death panels getting denied any medical care?
I'm all for elected officials keeping their promises, but if we are even going to pretend to adhere to the Constitution, the shutdown in order to prevent/ defund an already passed law is illogical.
Have you watched how the current regime has flouted the rule of lawl?
How did you miss it?
Outside of some partisan hacks there isnt really anyone saying 'the shutdown is a awesome idea!'. Its all just bad politics on all sides.


text:
I think she cried three times today, and I guess I'm ok with that. I mean sometimes life can just get completely overwhelming and you can't win for trying.
We are trying to make our way here in this new land, and it seems it's two steps forward, one step back.
Today it was to get our drivers license changed, which I've taken my time with because I've had the van insured in Saskatchewan. Too costly to have it updated here.
So we need an Alberta Drivers license to get Alberta health, and to get Alberta health, it seems we need an Alberta drivers license. It's like a torture circle. Â
I've changed provinces five times and each time it gets worse. I wonder how difficult it would be to have an identity card that I could take to any province and not have to do this stuff. I mean it's easier to go from one country to another in Europe, why can't Canada get caught up to this mobile world we live in.
Just grumbling a bit.
I did manage to find out how to get the gas stove pilot relit, twice. And the gas dryer seems to be working so far. So that's good.
But I'm soon off to bed as this flu is refusing to go away.
Today, I'm tired.
Well, that's a day i don't want to live againWednesday, February 11, 2009
1 comment
Subscribe to: Post Comments (Atom)
Copyright Randall Friesen. Powered by Blogger.


text:
Item description: This nhl Chicago Blackhawks product is by Fanatics Branded - Let your kiddo show his dedication to Patrick Kane with this Fanatics Branded Chicago Blackhawks Backer Name & Number T-shirt! It's stunning graphics will showcase his Chicago Blackhawks pride for all to see.... Learn More
Manufacturer: Fanatics Branded
Category: Chicago Blackhawks
You may also consider:
Copyright © 2018 - http://www.zonehockey.com/ All Rights Reserved.
Website design by Business Edge


text:
WILMINGTON, MA — Here are EIGHT highlights from the Wilmington Police Log for Friday, August 11, 2017:
- Police notified DPW that the Crescent Street sign and Doane Street sign on Broad Street are missing. (1:14am)
- A Lake Street caller reported her mailbox was struck sometime overnight. (6:08am)
- Police removed a deceased goose from the Ballardvale Office Park. (8:23am)
- Police notified DPW that the 25 MPH sign on West Street was struck by a vehicle and needs to be repaired. (8:54am)
- State Police reported a white Saturn sedan was involved in several crashes on the highway. Party struck a tractor trailer unit and guardrail several times. Vehicle exited the highway at Route 62 in Wilmington and stopped at Rick's Barber Shop. Police responded. Individual was not "fully alert." Fire Department transported man. State Police will handle investigation. Police checked area for any property damage in town on Route 62. (12:56pm)
- A caller reported a baby fox was in a driveway on Salem Street. (4:07pm)
- A Sheridan Road caller reported a man on a bicycle were stopping in front of houses in the area for long prolonged periods of time. Caller felt it was suspicious. Police responded. Bicyclist had two active warrants. Christopher Peter Hope (30, Wilmington) was arrested on two outstanding warrants — possession of Class A narcotics and shoplifting. (6:22pm)
- A caller reported a van parked in front of the St. Thomas entrance with two parties sitting on the front steps for the past 20 minutes. Police responded. The individuals were florists setting up for a wedding in the morning. (11:30pm)
(DISCLAIMER: This information is public information. An arrest does not constitute a conviction. Any arrested person is innocent until proven guilty.)
Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple's daily email newsletter HERE. Got a comment, question, photo, press release, or news tip?


text:
The Big Cook Review
Stock Up On Meals
If you have one to three friends who can cook and follow instructions, you can spend a day cooking up to 200 meals to divide between you.
The Big Cook gives the cook of the family the opportunity to stockpile nutritious meals so she (or he) can spend more time with the family or have some much needed down time.
No more staring into the chilly abyss of the fridge, wondering what to throw together at the last minute; or worse, loading everyone up in the car to eat out.
My Review
The Plan
Basically, The Big Cook details how to have an eight hour Saturday cook-off from their collection of tested and approved recipes. The Big Cook recommends a maximum of four, including yourself (the book also works for one cook). You can have 200 meals between you, and all ready to store in freezer in resealable freezer bags. The appendix in the back gives a sample four friend cook off, complete with grocery list, to get foodie cooks through the first attempt.
The Big Cooks
The Big Cook is the shared love child of three professional moms: Deanna Siemens, BScN, a mother of three; Lorelei Thomas, B. Ed, M.Ed, a mother of four; and Joanne Smith, B.Sc.H.Ec, RD, a mother of four. With this book, they have proven that you can lead a busy life and still put good food on the table for the family.
The Recipes
The Big Cook contains 24 beef recipes, 33 chicken recipes and 16 pork recipes. The easy to read instructions provide an ingredient breakdown for 1 meal, 4 meal, 6 meal or 8 meal with one meal feeding 4 - 6 people. A smaller family just means the opportunity for lunch leftovers. My only complaint is that I would have liked a section on meatless dishes.
The book is divided into three easy to read sections based on main meat ingredient. Each recipe offers methods for preparation, hints and serving suggestions. The last appendix gives a nutritional breakdown of calories, fat, sodium, carbs and protein. And it has a coil center so the book will open flat.
I highly recommend The Big Cook. It saves time, it saves money and most of all it saves the hassle of what to cook. If you have a big family, a large freezer for storage and are looking for nutritional no fuss recipes then you must add this book to your recipe cupboard. You may never use another book again. And, $1 from each book sale will be donated to "Food for the Hungry" and "World Compassion".
Get the Big Cook Book
The Big Cook can be ordered from the official Big Cook website.
Leave some warm fuzzies to go with my Big Cook lens.
You can rate this lens via the stars in the header.
Thank you for visiting!
Hello Cookbook Lovers!
No comments yet.