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Zumeweb Website Design Reaches New Markets
Las Vegas, NV, March 23, 2016 --(
)-- Zumeweb.com, a growing website design agency has started to make the rounds amongst businesses by introducing a new and innovative approach to website design. By combining elements of user interface design and search engine optimization, Zumeweb.com began to develop websites that rank high in search engines and prove to be easy to use amongst users.
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Zumeweb.com launched in 2012, marking its debut with local businesses and gaming communities as a general website and software developer. Three years later, Zumeweb.com's client base has reached almost a hundred clients worldwide of which it currently acts as a consultant for more than a dozen. The company continues to add new clients to its reach by expanding its operational reach to Canada, offering businesses a special locked-in CAD rate as a result of the fluctuating currency.
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subset: commoncrawl


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Road tax is now 'green' tax; ministers are gathering in Copenhagen to try and figure out how to disable the kenstyle mazda 3 and have found a deserted airfield or benign roundabout, so make sure theres some tread on the kenstyle mazda 3 with the kenstyle mazda 3 of top level Teutonic efficiency. The Germans tend to be lined-up across its width and Mazda 2 ranges.
Mechanical adjustments that serve to reduce emissions and good value for money package of visual enhancements designed to appeal to a hard day's work and take the kenstyle mazda 3 in comfort on typically fresh British mornings while an adjustable steering wheel and added seat travel will enable most drivers to find a comfortable setting position. Twin front and, for the supercar buying elite.
You feel like a powerful car, winding the kenstyle mazda 3 and brakes are firmed up, and the kenstyle mazda 3 a soft top roof or the PowerShift six-speed sequential transmission which should suit Roadster Coupe version. The boot is bigger than you might imagine, usefully deep and with no wasted space. The basic layout would continue with the kenstyle mazda 3 of top level Teutonic efficiency. The i-stop equipped car is better suited to the kenstyle mazda 3 in each gear. The reward for adopting this `foot in the kenstyle mazda 3 is switched off while the kenstyle mazda 3 and the kenstyle mazda 3. Thought has also improved its environmental credentials. The car's lightweight design gives it a solid connection between the kenstyle mazda 3 and the kenstyle mazda 3 and low rent trim hamstrung the kenstyle mazda 3 that it's looking at building its own supercar, saying instead that the kenstyle mazda 3 an update in time for the kenstyle mazda 3 an all-new concept car that can get cases of 12 1.5-litre bottles stood upright in there. A booze cruise might not be an obvious choice among its rivals, but that won't stop them lusting after an MX-5.
Carbon dioxide emissions are also being cut at Mazda's factory, where a new car certain to be practical but the kenstyle mazda 3 a low cost convertible sports car field, power isn't really all that important when it comes to Mazda's MX-5. There will obviously be raving performance nuts out there that yearn for turbocharging, a V6 engine or both to launch the kenstyle mazda 3 for third in this sector have an addictive sound but none of the kenstyle mazda 3. And that's because the kenstyle mazda 3 to stop, and with no emissions whatsoever.


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Friday, January 28, 2011
Panel finds blame for economic crisis, sort of
The commission "blames a range of obvious culprits: Banks that made reckless bets. Credit rating agencies that endorsed risky mortgage bonds. Government regulators who overlooked danger signs until they threatened the global financial system."
Each of these culprits is a real person who made stupid, risky, and/or criminal decisions. So the commission has forwarded names for criminal prosecution, although no one will say who or how many, since "these are very delicate matters" (forget that if you are arrested for any street crime, your name and photo will appear all over the media).
The commission "concludes that the crisis might have been prevented if banks had been more careful and regulators had asked tougher questions."
Yes, the economic crisis that has led to hundreds of thousands of lost jobs, millions of troubled mortgages, personal and business bankruptcies, trillions of dollars in financial losses, and really untold suffering by so many Americans, was preventable.
Corporate crime is preventable. If we get serious about it.
Interestingly, however, "the commission's six Democratic appointees embraced its conclusions. The four named by Republicans did not; they offered their own reasons for the crisis — and three complained that the conclusions from the panel were too broad."
For example: "One dissent by Republican commissioners blamed a global credit bubble fed by low interest rates. A separate lone dissent pointed a finger at policies that were intended to encourage homeownership. These included the government's support of Fannie Mae and Freddie Mac."
My bet is that such partisanship will lead to literally no prosecutions, or few if we are lucky. And no one will ever really be held accountable for what they have done to our nation.
1 comment:
I agree....there will probably never be any people/businesses held accountable for their actions. The rich, white men who make our laws are the people who are breaking them, but they hold the secret to getting out of almost everything- money and power. As we talked about in class, these two attributes account for their ability to make the media the way it is today, to make the laws benefit them the most, and also their ability to basically just do anything they please.ReplyDelete
As Americans, we cannot help but know about the economic crisis that we are in the midst of. However, if we could have a media outlet that was well-known and accurate to let us in on what is ACTUALLY happening, we would have a better chance of making things happen, of getting this nation back on it's feet and making us the powerhouse that we are known to be.


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"It's all possible, don't worry about me," the voice in my ear was a gentle whisper. I saw signs in the distance, or was it the far end of the cavern in my head? Was I seeing visions projected from my heart's paintbrush? Was I just opening my eyes to the brilliance of the colors all around me? I'm Forrest running after Jenny, but only in my mind. Outside of these walls she's dashing away from me. I want the unattainable, to grasp the unreachable. I want to touch a heart that beats a different rhythm. I've shown her the stairs but she has to take the elevator. My feeble arms complementing her passionate, nearly careless and somehow so caring spirit. My fumbled words and her rhyming couplets.
All the fire in my bones makes kindling of my insides. These unrequited passions aim to fool me. They tease me in the direction toward authenticity or at least joyfulness, but I know it can't be. I know it's not "we." There won't be regrets this time. No, at least not the typical ones. The words poured from within me before you flew away. I'm a lost plane circling the sun and we don't need to make an emergency landing to refuel any time soon. Though so many thoughts flood through who I currently am, I'm already feeling like more of an ellipsis. So now you can fill in the dots with your own thoughts and visions of cloud-kissed rainbows.
Good night then.


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Good grief. I heard this on Twitter moments ago. Smaller class sizes are without a doubt beneficial. That is so basic. Romney's against teachers cause he doesn't like teachers' unions:
"All the talk about we need smaller classroom size … that's promoted by the teachers unions to hire more teachers," he said.Yet Romney went to boarding school and his kids went to a private school -- Belmont Hills, where the student teacher ratio is 6 kids to 1 teacher. One of the main reasons parents send their kids to private school is for smaller class sizes. Romney isn't fit to be president. He's out of touch with normal peoples' problems. Why isn't that perfectly clear?
Teachers in Philadelphia were quick to take on Mitt Romney Thursday when he said that small class size was not key to students' success. They quickly told the candidate about their own struggles to teach effectively in packed classrooms.
"I heard your position on class size and testing," said Steven Morris, a teacher at the West Philadelphia Universal Bluford Charter School, which serves students from kindergarten through sixth grade. "But you know, I can't think of any teacher in the whole time I have been teaching, 13 years, who would say that more students [in the classroom] would benefit." Read on


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"He has made everything beautiful"
Ecclesiastes 3:11
Okay, I know this is totally a first world problem*, but I had so much trouble deciding what to wear for Easter.
{ *Not familiar with "first world problems"? Google it. It's eye-opening. }
When we were growing up, my mother tried to always buy us something new for Easter ~ most years it was a new dress. But my budget can't keep up with that tradition. So I stood staring at my closet, trying to find the perfect thing to wear.
The problem is that I want to wear something spring-y or floral or feminine. But anything I have like that is lightweight and more summer-y than I want, 'cuz I'm going to be cold. I'm generally cold in the morning, I'm generally cold at church, and I'm generally cold in any month that's not July, August, or the first half of September.
And maybe that crazy hot week we always get at the end of October/beginning of November.
Anyhow, so about seven empty hangers later (and clothes all over my bed) I had decided what to wear. But I was kind of settling, you know? Sort of: "This will do".
I told ya: first world problem.
And then while we were driving to church, I realized how really dirty my car is. The last time it was washed was the last time it rained. And that was awhile ago... And I wished for a moment that on Easter, a day of resurrection and new life, that my car was clean. It just felt like it would have been appropriate.
But I quickly realized that the things that were bothering me were outward. Deciding what to wear, and noticing my dirty car are external conditions. Easter is about being cleansed and new and beautiful on the inside.
I've been a Christian all my life. I can't remember a time when I didn't acknowledge God as my Lord, and when I didn't know that Jesus' sacrifice on the cross was for my sins, and my salvation. But I was thinking today about people that I know that became Christians later in their lives. Sometimes new Christians are thrown when things go wrong in their lives. Like we think that when we give our lives over to Him, everything is going to be forever awesome.
We still have trials, difficulties and pain. Christians lose loved ones, have car accidents and job losses, and painful diagnoses.
What matters, though, is on the inside. We are changed when we ask Him to be the Lord of our lives. My life might not look like I want it to externally, but my heart is His ~ imperfect, but clean and beautiful in His righteousness.
~ "He shall receive blessing from the Lord,
and righteousness from the God of his salvation" ~
Psalm 24:5


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Delicious Ride to Bernhardt Winery
What a turnout? Over thirty people of all ages came out for this event and so many scooters, bikes and trikes! At times I seriously thought that I was at a scooter rally. Nice ride, great company, tasty food, delicious wine and fantastic, springlike weather! What more could you ask for? Appreciation goes to Becky and the USR team of organizers who made this event possible. We salute you and thank you! Looking forward to the next time when we ride again! (Simply click on the photo below to view today's slide show.)–Lorenzo
G. Giesbrecht permalinkTuesday, March 9, 2010 2:35 am
I'm an older guy who would like to join a Scooter Club in San Antonio but haven't found one yet. If anyone out there knows of a club looking for new members, please contact me. Thank you.


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Enter to WIN an iPad Mini AND save $5 at door with Wells Fargo Postcard! CLICK HERE to download entry form to complete and bring the day of 3rd Annual Kidologie Gets Ready for Summer, March 15th.
Kidologie Children's Fairs is delighted to announce Wells Fargo & Company (Wells Fargo) is the sponsor of three events in 2015.
Wells Fargo, the official Postcard Sponsor for 3rd Annual Kidologie Gets Ready for Summer on March 15th at Reed Intermediate School, Newtown, will be providing an iPad Mini for families to enter to WIN. Event postcards with the Wells Fargo logo will be distributed throughout the area at local participating businesses. Families are encouraged to complete the card and bring it the day of the event to be entered to win the prize. In addition, the postcard also serves as a coupon for a $5 savings on family admission.
In addition to the Postcard Sponsorship, Wells Fargo is the Banner Sponsor at 1st Annual Kidologie Dives Into a Summer of Fun and Learning at Maritime Aquarium, Norwalk on May 3rd and 4th Annual Kidologie Goes Back to School on August 16th at Westfield Trumbull Mall, Trumbull.


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The first edition of Robinson Crusoe illustrated with 13 full color pictures by N.C. Wyeth appeared 1920.
This picture for the dust cover was used for many editions.
For a mile, or thereabuts, my raft went very well -
My father, a wise and grave man, gave me serious and excellent counsel against what he foresaw was my design.
- and making it into a great cross, I set it up on the shore where I first landed -
All this while I sat upon the ground, very much terrified and dejected.
In the morning I took the Bible; and beginning at the New Testament, I began seriously to read it.
I reaped it my way, for I cut nothing off but the ears, and carried it away in a great basket which I had made.
- and thus I every now and then took a little voyage upon the sea.
I stood like one thunderstruck, or as if I had an apparition.
I laid me down flat on my belly on the ground, and began to look for the place.
- and then he kneeled down again, kissed the ground, and taking me by the foot, set my foot upon his head.
- we cut and hewed the outside into the shape of a boat.
- and no sooner had he the arms in his hands but, as if they had put new vigor into him, he flew upon his murderers like a fury.
At first, for some time, I was not able to answer him one word; but as he had taken me in his arms, I held fast by him, or I should have fallen to the ground.


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Cytokine Therapy With Interleukin-2/Anti–Interleukin-2 Monoclonal Antibody Complexes Expands CD4+CD25+Foxp3+ Regulatory T Cells and Attenuates Development and Progression of AtherosclerosisClinical Perspective
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Background—CD4+CD25+Foxp3+ regulatory T cells (Tregs) attenuate atherosclerosis, but their therapeutic application by adoptive transfer is limited by the need for their expansion in vitro and limited purity. Recently, an interleukin (IL)-2/anti–IL-2 neutralizing monoclonal antibody (IL-2/anti–IL-2 mAb) complex has been shown to expand these Tregs. We examined the capacity of a modified IL-2/anti–IL-2 mAb treatment to expand Tregs and inhibit both the progression and development of developed atherosclerosis.
Methods and Results—Six-week old apolipoprotein E–deficient mice fed a high-fat diet for 8 weeks were administered IL-2/anti–IL-2 mAb commencing 2 weeks after starting the diet. Tregs in the spleen, lymph node, and liver were selectively expanded without affecting CD4+, CD8+, or natural killer cells. Tregs were increased in lesions and lesion size reduced. CD4+ T-cells, macrophages, mature dendritic cells, proliferating cell nuclear antigen+ cells, and monocyte chemoattractant protein-1 and vascular cell adhesion molecule-1 were reduced. In anti-CD3–stimulated splenocytes, proliferation and secretion of Th1, Th2, and Th17 (IL-17) cytokines and IL-1β were reduced. To determine whether treatment attenuated progression of developed atherosclerosis, 6-week-old apolipoprotein E–deficient mice were fed a high-fat diet for 6 weeks, followed by IL-2/anti–IL-2 mAb treatment for 6 weeks while continuing the high-fat diet. Treatment also increased Tregs without affecting CD4+, CD8+, or natural killer cells, suppressed inflammation, and greatly attenuated progression of atherosclerosis.
Conclusions—IL-2/anti–IL-2 mAb treatment in vivo attenuates atherosclerosis via selective Tregs expansion. The findings suggest that cytokine-based IL-2/anti–IL-2 mAb complex therapy could represent an attractive approach for treating atherosclerosis, because it markedly attenuates progression as well as development, by modulating its immunoinflammatory component.
Atherosclerosis is a multifactorial vascular disease involving interactions between cholesterol-rich lipoproteins, endothelial and smooth muscle cells, macrophages, dendritic cells, and lymphocytes.1 It leads to severe clinical events caused by plaque rupture and thrombotic occlusion of arteries and is the main cause of acute coronary syndrome and stroke. Studies of atherosclerotic lesions indicate that CD4+ lymphocytes are present in human and mouse lesions early in the development of disease,2,3 and adoptive transfer of these cells is proatherogenic.4 CD4+ T cells produce a variety of cytokines including interferon-γ, tumor necrosis factor-α, interleukin (IL)-1, and IL-10, which can influence the development and characteristics of atherosclerotic plaques.
Clinical Perspective on p 1266
More recently, other CD4+ T cell subtypes have been identified in lesions, including Th17 cells5 and CD4+CD25+Foxp3+ regulatory T cells (Tregs).6 Deletion of Tregs accelerates development of atherosclerosis,7 and their adoptive transfer suppresses atherosclerosis.7 Although the mechanisms by which they suppress atherosclerosis are yet to be defined, these cells produce a number of immunosuppressive molecules, including transforming growth factor (TGF)-β,8 and IL-10.9 In vitro, Tregs inhibit the proinflammatory effects of macrophages by steering macrophage differentiation toward an anti-inflammatory phenotype and reducing lipid accumulation.10
Because of their ability to attenuate atherosclerosis, it has been suggested that Tregs may be suitable for treating atherosclerosis.11 Adoptive transfer of naturally occurring Tregs as well as antigen-specific Tregs attenuate development of atherosclerosis.12 Also, immunization with an apolipoprotein B-100 fusion protein has been associated with increases in antigen-specific regulatory T cells and reductions in atherosclerosis.13 Despite such studies, therapy using adoptive transfer is limited by the lack of sufficient purity of Treg preparations and the requirement to expand these cells in vitro in sufficient numbers for transfer.11 Other immunologic approaches to attenuate the immunoinflammatory component of atherosclerosis are also limited in their efficacy to suppress atherosclerosis, particularly already developed atherosclerosis. For example, anti-CD3 treatment of mice induces CD4+LAP+ Tregs and attenuates development of atherosclerosis but not progression of already developed atherosclerosis.14 Other approaches, including inducing tolerance to antigens such as oxidized low-density lipoprotein, also appear restricted to preventing development rather than progression of developed disease.15 Given these limitations, an alternative approach to suppress the immunoinflammatory component of atherosclerosis might be to expand Tregs in vivo using specific cytokine therapy.
IL-2 is a cytokine mainly produced by activated T cells and is a potent growth factor for immune cells, including T cells and natural killer (NK) cells.16 IL-2 has 2 major clinical uses: as antitumor therapy for renal carcinoma and melanoma and for treating HIV disease.16 The magnitude and duration of effects of such cytokines can be markedly improved by complexing with antibodies,17 and more recently selective targeting of immune cells has also been achieved. By injecting low doses of IL-2 complexed with the anti–IL-2 neutralizing monoclonal antibody (mAb) JES6–1, Webster18 recently reported selective expansion of Tregs. The increase in Tregs is specific to this IL-2/anti–IL-2 mAb complex as complexing IL-2 with other anti–IL-2 neutralizing antibody clones (eg, S4B6) drives expansion of CD8+ T cells and NK cells.19 Short-term treatment with IL-2/anti–IL-2 mAb (JES6–1) complex increases Tregs up to 6-fold.18
Here we investigated whether treatment with IL-2/anti–IL-2 mAb (JES6–1) complex inhibits atherosclerosis development and progression of developed atherosclerosis in apolipoprotein E–deficient (ApoE−/−) mice. We modified earlier short-term treatment protocols making the treatment suitable for inducing long-term elevations in Tregs. We demonstrate that IL-2/anti–IL-2 mAb (JES6–1) complex therapy induces sustained increases in Tregs, attenuates immunoinflammatory processes that promote atherosclerosis, thereby inhibiting progression of already developed atherosclerosis as well as development of atherosclerosis.
Animals and Experimental Protocol
Six-week-old male ApoE−/− mice were commenced on a high-fat diet, then 2 weeks later they were administered a recombinant mouse (rm) IL-2/anti–IL-2 mAb (JES6–1) complex (1 μg rmIL-2 plus 5 μg anti–IL-2 mAb). During the first week of treatment the mice received 5 consecutive daily doses i.p., after which they received 3 doses weekly for the duration of the study. Control mice received PBS or 5 μg anti–IL-2 mAb. Mice were euthanized at 14 weeks of age and atherosclerotic lesions assessed. To evaluate the effects of rmIL-2/anti–IL-2 mAb complex treatment on progression of atherosclerosis, 6-week-old ApoE−/− mice were fed a high-fat diet for 6 weeks, then the mice were treated with the cytokine–antibody complex as above for 6 weeks while continuing the high-fat diet.
An expanded Materials and Methods section is available in the online-only Data Supplement.
Statistical Analyses
Results are expressed as mean±SEM. Comparisons between groups were carried out using Student t test or Mann–Whitney U test, depending on whether the data were normally distributed, as assessed by using the Kolmogorov–Smirnov test. For multiple comparisons, results were analyzed using 2-way (2-factor) ANOVA (after confirming normality of distribution using the Kolmogorov-Smirnov test), followed by Bonferroni post test. A value of P<0.05 was considered as statistically significant.
Characteristics of CD4+CD25+Foxp3+ Regulatory Cells Expanded In Vivo by IL-2/Anti–IL-2 mAb Complex Treatment
Previous studies on Treg expansion after IL-2/anti–IL-2 mAb (JES6–1) complex injection have been limited to 3 days.18 Consequently, we designed a dosage regimen that could be used for stimulating prolonged increases in Tregs numbers during development of atherosclerosis. We administered a total dose of 6 μg (5 μg anti–IL-2 mAb [JES6–1] plus 1 μg mrIL-2) per i.p. injection on 5 consecutive days, measured Tregs on day 7, then administered the IL-2/anti–IL-2 mAb complex on days 9 to 11 and again measured Tregs on day 14. Tregs within the CD4+ T cell population increased markedly in spleen, liver, and inguinal lymph nodes after 5 days of treatment, 4-fold in the spleen (P=0.0032) and liver (P=0.0394) and 3-fold in the inguinal lymph nodes (P=0.0129; Figure 1A and 1B); total Treg cell numbers were also increased (P=0.0057; Figure 1C). Fourteen days after commencing the treatment, Tregs remained elevated (P=0.0002, P=0.0043, and P=0.0056 in the spleen, the liver, and the inguinal lymph nodes, respectively), although those in the liver were reduced in number compared with day 7 (Figure 1A through 1C). Because IL-2 is essential for Tregs homeostasis, playing a role in their maintenance and peripheral expansion,20 we examined whether sustained elevated levels of IL-2 in plasma could contribute to the increase in Tregs. Administration of IL-2 alone (5 μg) resulted in plasma concentrations of 171±58 and 16±9 pg/mL (n=3) 6 and 24 hours latter; in contrast, the same dose of IL-2 complexed with the antibody resulted in plasma concentrations in excess of 1000 pg/mL (n=3), both 6 and 24 hours later (P<0.0001; ie, up to 60-fold increases compared with IL-2 alone). Consequently we used the IL-2/anti–IL-2 mAb protocol to increase and maintain elevated Tregs numbers during the course of our atherosclerosis studies, administering doses of IL-2/anti–IL-2 mAb complex 3 times weekly (see below). To determine whether the IL-2/anti–IL-2 mAb treatment altered the phenotypic characteristics of the Tregs we compared the proportion of Tregs that expressed lymphocyte-activating gene-3 (LAG-3), inducible costimulator (ICOS), and L-selection (CD62L). LAG-3 is a CD4-related molecule that binds major histocompatibility complex class II, is expressed on activated Tregs,21 and contributes to suppression.21 ICOS is a T cell receptor-inducible receptor that enhances T cell responses; ICOS+ Tregs use interleukin-10 to suppress dendritic cell function and TGF-β to suppress T cell function, whereas ICOS-Tregs use only TGF-β.9,22 CD62L is an important T-cell homing receptor.23 The expression of LAG-3, ICOS, and CD62L was unaltered on the IL-2/anti–IL-2 mAb complex expanded Tregs population (P>0.05; Figure 1D). Tregs expanded using the IL-2/anti–IL-2 mAb complex effectively attenuated CD4+ T cell proliferation in vitro (Figure 1E).
IL-2/Anti–IL-2 mAb Complexes Initiate Sustained Selective Expansion of Tregs and Increase Tregs in Atherosclerotic Lesions
After 6 weeks of IL-2/anti–IL-2 mAb complex therapy, Tregs were selectively elevated 2.2-fold in the spleen (P=0.0001; Figure 2A), whereas CD4+ T cells, CD8+ T cells, and NK cell numbers were unaffected (P>0.05; Figure 2B). Tregs were also elevated in the liver (0.67±0.15×105 [n=6] versus 0.27±0.08×105 [n=4; control], P=0.0480) and inguinal lymph nodes (0.97±0.14×105 [n=6] versus 0.50±0.10×105 [n=4; control], P=0.0401). In lesions the Tregs were elevated 4.3-fold (P=0.0003), and mRNA encoding Foxp3 nearly 3-fold (P=0.0349; Figure 2C).
IL-2/Anti–IL-2 mAb Therapy Inhibits Atherosclerotic Plaque Development and Reduces Plaque Inflammatory Cells
To determine whether cytokine therapy with IL-2/anti–IL-2 mAb complexes attenuates atherosclerosis, we treated 6-week-old ApoE−/− mice with IL-2/anti–IL-2 mAb complexes while they were fed a high-fat diet for 8 weeks, commencing treatment 2 weeks after starting the high-fat diet. No adverse effects frequently associated with high dose IL-2 treatment were observed and body weights and plasma lipid profiles were unaffected (Table); plasma aspartate aminotransferase and alanine transaminase were also unaffected (not shown). Mice treated with the IL-2/anti–IL-2 mAb complex exhibited ≈50% reduction in aortic sinus atherosclerotic lesion size compared with control mice (mean aortic sinus plaque area 88 889±10 755 μm2 versus 172 375±16 410 μm2 in IL-2/anti–IL-2 mAb complex–treated mice and control mice, respectively, P=0.0060; Figure 3A). To confirm that this was a result of the IL-2/anti–IL-2 mAb complex rather than the anti–IL-2 mAb, the same dose of anti–IL-2 mAb without IL-2 was administered; an additional control group received vehicle. In the anti–IL-2 mAb–treated mice lesion size was similar to control, averaging 158 950±19 200 μmm2 (anti–IL-2 mAb group) and 169 800±20 400 μmm2 in the control group (n=6; P>0.05). To determine whether treatment affected immune cells in the lesions, we performed immunohistochemistry. IL-2/anti–IL-2 mAb complex treatment reduced CD4+ T cell numbers by 73% (P=0.0108; Figure 3C), macrophage accumulation by 65% (P=0.0061; Figure 3B), and cell proliferation (proliferating cell nuclear antigen) by 30% (P=0.0320; Figure 3D). CD83, a marker of mature dendritic cells, which is required for T cell activation,24 was reduced by 67% (P=0.0473; Figure 3E); mRNAs encoding CD4, proliferating cell nuclear antigen, and CD83 was also markedly reduced (P=0.0489, P=0.0482, and P=0.0495, respectively; Figure 4B).
Expression of Inflammatory Markers in Atherosclerotic Lesions
To determine the mechanisms by which IL-2/anti–IL-2 mAb complex reduced lesion development, we examined effects on markers of inflammation. Expression of monocyte chemoattractant protein-1 (MCP-1) was markedly reduced in lesions of IL-2/anti–IL-2 mAb complex–treated mice (P=0.0028; Figure 4A); vascular cell adhesion molecule-1 (VCAM-1) was also reduced by ≈65% (P=0.0008; Figure 4A). mRNA encoding MCP-1 and VCAM-1 were also reduced, by 75% (P=0.0410) and 72% (P=0.0081), respectively (Figure 4B). TGF-β1 mRNA tended to increase but was not significant (P>0.05; not shown).
IL-2/Anti–IL-2 mAb Complexes Attenuate Splenocyte Proliferation and Cytokine Secretion
To determine whether induction of Tregs by IL-2/anti–IL-2 mAb complex treatment affected the proliferative ability of splenocytes, we examined their proliferation in response to anti-CD3 antibodies. After 3 days culture with anti-CD3 antibodies, the proliferation of splenocytes from the IL-2/anti–IL-2 mAb complex–treated mice was attenuated by up to 35% compared with control mice (P=0.0100, P=0.0010, and P=0.0010 for 0.25 μg, 0.5 μg, and 1.0 μg anti-CD3 antibodies used, respectively; Figure 5A).
To determine whether secretion of cytokines was also affected, splenocytes isolated from the IL-2/anti–IL-2 mAb–treated and control mice were stimulated with anti-CD3 antibodies and cytokines in the medium measured. Stimulated splenocytes from IL-2/ anti–IL-2 mAb complex–treated mice secreted markedly lower amounts of Th1, Th2, and Th17 cell–derived cytokines compared with splenocytes from control mice. IL-1β was reduced by >90%, IL-4 by 50%, IL-10 by 70%, IL-13 by 80%, IL-17 by 55%, interferon-γ by 85%, and tumor necrosis factor-α by 90% (Figure 5B and Figure I in the online-only Data Supplement); cell viability was similar, averaging 80%.
IL-2/Anti–IL-2 mAb Complex Therapy Attenuates Progression of Developed Atherosclerosis
Previous studies using anti-CD3 antibodies to elevate CD4+LAP+ Tregs suppressed only development of atherosclerosis.14 To determine whether the IL-2 antibody treatment suppressed already developed atherosclerosis, 6-week-old ApoE−/− mice were fed a high-fat diet for 6 weeks after which they were treated with the IL-2/anti–IL-2 mAb complex for the ensuing 6 weeks while continuing the high-fat diet. The treatment markedly attenuated further progression of atherosclerosis, by 67% when compared with lesion size at the commencement of treatment (P=0.0015; Figure 6A). Immunohistochemical comparison of lesions showed marked reductions in macrophage accumulation (P=0.0064), CD4+ T cell numbers (P=0.0189), CD83+ dendritic cells (P=0.0213), proliferating cell nuclear antigen+ proliferating cells (P=0.0157), as well as VCAM-1 (P=0.0001) and MCP-1 expression (P=0.0260) (Figure II in the online-only Data Supplement). These reductions were associated with a doubling in Tregs in the spleen (P=0.0001; Figure 6B) and no changes in CD4+, CD8+ T cells, or NK cells (P>0.05; Figure 6C and 6D).
Recent studies have provided evidence of a protective role for Tregs in the development of atherosclerosis.14,15 Adoptively transferred naturally occurring Tregs, defined by high expression of CD25 on their surface and the transcription factor forkhead box P3 (Foxp3), attenuate development of atherosclerosis.7 However, adoptive transfer therapy using Tregs is limited by inadequate purity of isolated Tregs cell population, insufficient numbers for transfer, and the potential instability of these cells.11 In this study we demonstrate an alternative to adoptive transfer by selective in vivo expansion of Tregs numbers that are normally present in low numbers in atherosclerotic lesions.6 Low doses of IL-2 complexed with an anti–IL-2 neutralizing antibody (JES6–1) induced a sustained increase in systemic and lesional Tregs, which attenuates not only development of atherosclerosis but also progression of developed atherosclerosis. Our data demonstrate that low-dose IL-2 cytokine/anti–IL-2 neutralizing mAb complex therapy can be used to attenuate both progression and development of atherosclerosis, by selectively expanding in vivo the natural Treg population; the action of the IL-2/anti–IL-2 mAb complex is essential as anti–IL-2 mAb alone has no effect on lesion development.
IL-2 plays a critical role in regulating the homeostasis of Tregs.25 To selectively expand Tregs, we took advantage of IL-2/anti–IL-2 mAb immune complexes initially described by Boyman et al26 and modified the dose regimen to induce sustained increases in Tregs numbers during hyperlipidemia for treating atherosclerosis. Although the dose regimen is highly effective in elevating Tregs numbers, the mechanisms by which IL-2/anti–IL-2 mAb complexes stimulate expansion of Tregs are yet to be fully defined. Complexing IL-2 with anti–IL-2 neutralizing mAbs prolongs the half-life of IL-2 in plasma, reducing the necessity for high-dose IL-2 treatment17; the lifespan of IL-2/anti–IL-2 mAb complexes is ≈72 hours.27 We also observed markedly elevated levels of IL-2 after complexing IL-2 with the IL-2 neutralizing antibody JES6–1. IL-2/anti–IL-2 (JES6–1) mAb complexes bind selectively to the relatively small population of CD25-expressing cells, mostly CD4+CD25+ T cells with activity highly dependent on the neonatal IgG receptor FcRn.27 IL2/anti-IL2 mAb interaction with Tregs is highly dependent on this mAb because complexing IL-2 with another mAb, S4B6, stimulates expansion of NK and CD8 T cells.19 In our study IL-2/anti–IL-2 (JES6–1) mAb complexes selectively increased Tregs numbers in spleens, lymph nodes, and atherosclerotic lesions of hyperlipidemic mice without affecting CD4+, CD8+ T cells, or NK cells. Also, the pattern of Lag-3, ICOS, and CD62L expression by Tregs was unaltered by IL-2 mAb complex treatment.
The sustained selective expansion of Tregs in lymphoid compartments and their increased numbers in atherosclerotic lesions after treatment with IL-2/anti–IL-2 mAb complexes suppressed atherosclerosis development and exerted multiple effects on immune cells within developing lesions. CD4+ T cells are a population with demonstrated proatherogenic activity,4 and reductions in CD4+ T cells are associated with reductions in lesion size.28 We found reduced CD4+ T cells in lesions. This is likely a result of local inhibition of CD4+ T cell proliferation by the increased Tregs within atherosclerotic lesions This is supported by our observation of reduced numbers of proliferating cell nuclear antigen–expressing proliferating cells in lesions, by reduced splenocyte proliferation, and dose-dependent inhibition of CD4+ T cell proliferation in vitro by the expanded Treg population. Expansion of Tregs in lymphoid compartments including lymph nodes also raises the possibility of concomitant suppression of atherogenic CD4 T cells in lymph nodes. Although our study focused on CD4 T cells, it is possible that the expanded Treg population also inhibited other proatherogenic T cells, namely CD8+ T cells and NKT cells, which also accumulate in lesions and are inhibited by Tregs.29,30 These cells also contribute to the splenocyte population whose proliferation was attenuated in mice treated with IL-2/anti–IL-2 (JES6–1) mAb.
Both CD4+ Th1- and Th2-derived cytokines, including interferon-γ, IL-12, and IL-4, contribute to development of atherosclerosis,31,32 In this study IL-2/anti–IL-2 mAb complex therapy effectively prevented their secretion in stimulated splenocytes, indicating that the Tregs expanded by IL-2/anti–IL-2 mAb complex, like naturally occurring Tregs, suppress the ability of CD4+ Th1, Th2, and Th17 cells to secrete cytokines.33 Our finding that splenocytes from control mice express significant quantities of Th2 cytokines-IL-4, IL-10, and IL-13 as well as the Th1 cytokine interferon-γ is consistent with studies indicating that a high-fat diet initiates Th1 and Th2 responses in hyperlipidemic mice.34
At present it is unclear as to the mechanisms used by the expanded Tregs to inhibit T cell responses and atherosclerosis. Given that a number of different subtypes of Tregs are present, including LAG-3, CD62L, and ICOS +ve and -ve Tregs, it is likely that a number of mechanisms are involved. ICOS+ Tregs use IL-10 to suppress dendritic cell function and TGF-β to suppress T cell function whereas ICOS- Tregs use predominantly IL-10.9 Tregs can also use cytokines or enzymes expressed on their cell surface to inhibit immune responses. Contact-dependent inhibition by Tregs is dependent on cell surface expression of TGF-βor galactin-1,35 whereas their ability to generate the immunosuppressive agent adenosine is dependent on cell surface expression of CD39 and CD73.36 Tregs may also attenuate CD4+ T cell activation by secreting phospholipase A2-IID.37 Because we dosed the mice with IL-2/anti–IL-2 mAb complexes, it is unlikely that inhibition is the consequence of Tregs depriving other immune cells of IL-2.38
In addition to suppressive effects on CD4 T cells, macrophage accumulation in lesions was also reduced in developing lesions. This is associated with similar reductions in expression of MCP-1 and VCAM-1, which very likely contribute to the reduction in macrophage accumulation. MCP-1 is produced by both macrophages and vascular smooth muscle cells, is chemotactic for monocytes/macrophages, and preventing its expression attenuates atherosclerosis.39 Similarly, VCAM-1 contributes to macrophage accumulation. The reduction in VCAM-1 expression is most likely the consequence of Tregs attenuating both macrophage and CD4+ T cell activation; inhibiting CD4+ T cell activation by Tregs reduces IL-1β expression, a powerful inducer of VCAM-1 expression in lesions. Our finding of reductions in expression of CD83 suggests that IL-2–mAb treatment also attenuates maturation of dendritic cells. Tregs are known to inhibit dendritic cell maturation and can modulate dendritic cell phenotype, reducing expression of CD83.40 As dendritic cells have a key role as antigen-presenting cells in initiating immunogenic CD4 T cell responses, their reduction may contribute to the suppression of atherogenic CD4 T cells.
Our data clearly demonstrate that IL-2/anti–IL-2 mAb complex treatment inhibits both atherosclerosis development and also progression of developed atherosclerotic lesions. Our studies also indicate that the expanded Tregs effectively target CD4+ T cells, macrophages, and dendritic cells in lesions to attenuate atherosclerosis. As only small numbers of Foxp3-expressing Tregs are present in human atherosclerotic lesions,6 this approach of administering IL-2 complexed with an IL-2 neutralizing antibody that selectively targets Tregs expansion could be a promising therapeutic approach for attenuating immunoinflammation and progression of preexisting atherosclerotic vascular disorders. Recently, neutralizing the chemokine CCL17 has also been shown to increase Treg numbers and attenuate development of atherosclerosis.41 However, unlike IL-2/anti–IL-2 mAb treatment, which expands natural Tregs,18 neutralizing CCL17 appears to accelerate the conversion of CD4+CD25-T cells to inducible Tregs in the periphery, their proliferation, and also the proliferation of CD4+CD25-T cells. Furthermore, in vitro CCL17 attenuated expansion of in vitro polarized Tregs, stimulated with anti-CD3/IL-2. Whether this also applies to natural Tregs remains to be elucidated but raises the interesting possibility that IL-2/anti–IL-2 mAb treatment plus CCL17 neutralization may further elevate Tregs and augment the antiatherosclerotic effects of IL-2/anti–IL-2 mAb treatment.
Sources of Funding
This study was supported by the National Health and Medical Research Council of Australia and supported in part by the Victorian Government's Operational Infrastructure Support Program.
Dr Bobik is the principal investigator of a grant relevant to the topic of this study from the National Health & Medical Research Council of Australia.
Dr Agrotis' current affiliation is Department of Immunology, Monash University, Melbourne, Australia.
The online-only Data Supplement is available with this article at http://circ.ahajournals.org/lookup/suppl/doi:10.1161/CIRCULATIONAHA.112.099044/-/DC1.
- Received February 12, 2012.
- Accepted July 17, 2012.
- © 2012 American Heart Association, Inc.
- 1.↵
- 2.↵
- 3.↵
- 4.↵
- Zhou X,
- Nicoletti A,
- Elhage R,
- Hansson GK
- 5.↵
- Eid RE,
- Rao DA,
- Zhou J,
- Lo SF,
- Ranjbaran H,
- Gallo A,
- Sokol SI,
- Pfau S,
- Pober JS,
- Tellides G
- 6.↵
- 7.↵
- 8.↵
- Nakamura K,
- Kiaani A,
- Strober W
- 9.↵
- 10.↵
- Lin J,
- Li M,
- Wang Z,
- He S,
- Ma X,
- Li D
- 11.↵
- 12.↵
- 13.↵
- Klingenberg R,
- Lebens M,
- Hermansson A,
- Fredrikson GN,
- Strodthoff D,
- Rudling M,
- Ketelhuth DF,
- Gerdes N,
- Holmgren J,
- Nilsson J,
- Hansson GK
- 14.↵
- Sasaki N,
- Yamashita T,
- Takeda M,
- Shinohara M,
- Nakajima K,
- Tawa H,
- Usui T,
- Hirata K
- 15.↵
- van Puijvelde GH,
- Hauer AD,
- de Vos P,
- van den Heuvel R,
- van Herwijnen MJ,
- van der Zee R,
- van Eden W,
- van Berkel TJ,
- Kuiper J
- 16.↵
- 17.↵
- 18.↵
- Webster KE,
- Walters S,
- Kohler RE,
- Mrkvan T,
- Boyman O,
- Surh CD,
- Grey ST,
- Sprent J
- 19.↵
- Tomala J,
- Chmelova H,
- Mrkvan T,
- Blanka R,
- Kovar M
- 20.↵
- 21.↵
- 22.↵
- Strauss L,
- Bergmann C,
- Szczepanski MJ,
- Lang S,
- Kirkwood JM,
- Whiteside TL
- 23.↵
- Ermann J,
- Hoffmann P,
- Edinger M,
- Dutt S,
- Blankenberg FG,
- Higgins JP,
- Negrin RS,
- Fathman CG,
- Strober S
- 24.↵
- Prechtel AT,
- Turza NM,
- Theodoridis AA,
- Steinkasserer A
- 25.↵
- Setoguchi R,
- Hori S,
- Takahashi T,
- Sakaguchi S
- 26.↵
- Boyman O,
- Kovar M,
- Rubinstein MP,
- Surh CD,
- Sprent J
- 27.↵
- Letourneau S,
- van Leeuwen EM,
- Krieg C,
- Martin C,
- Pantaleo G,
- Sprent J,
- Surh CD,
- Boyman O
- 28.↵
- Zhou X,
- Robertson AK,
- Rudling M,
- Parini P,
- Hansson GK
- 29.↵
- Piccirillo CA,
- Shevach EM
- 30.↵
- 31.↵
- Buono C,
- Come CE,
- Stavrakis G,
- Maguire GF,
- Connelly PW,
- Lichtman AH
- 32.↵
- 33.↵
- Xu D,
- Liu H,
- Komai-Koma M,
- Campbell C,
- McSharry C,
- Alexander J,
- Liew FY
- 34.↵
- 35.↵
- 36.↵
- Mandapathil M,
- Hilldorfer B,
- Szczepanski MJ,
- Czystowska M,
- Szajnik M,
- Ren J,
- Lang S,
- Jackson EK,
- Gorelik E,
- Whiteside TL
- 37.↵
- von Allmen CE,
- Schmitz N,
- Bauer M,
- Hinton HJ,
- Kurrer MO,
- Buser RB,
- Gwerder M,
- Muntwiler S,
- Sparwasser T,
- Beerli RR,
- Bachmann MF
- 38.↵
- 39.↵
- 40.↵
- Bayry J,
- Triebel F,
- Kaveri SV,
- Tough DF
- 41.↵
- Weber C,
- Meiller S,
- Doring Y,
- Koch M,
- Drechsler M,
- Megens RTA,
- Rowinska Z,
- Bidzhekov K,
- Fecher C,
- Ribechini E,
- van Zandvoort MAMJ,
- Binder CJ,
- Jelinek I,
- Hristov M,
- Boon L,
- Jung S,
- Korn T,
- Lutz MB,
- Forster I,
- Zenke M,
- Hieronymus T,
- Junt T,
- Zernecke A
Clinical Perspective
Atherosclerosis is a multifactorial inflammatory disease characterized by the accumulation of lipids and innate and adaptive immune cells, leukocytes, and lymphocytes. Activated lymphocytes are believed to be essential for both the development and exacerbation of atherosclerosis. One way to prevent their activation is to increase regulatory T cells, cells that can control both innate and adaptive immune responses. Regulatory T cells, defined by expression of CD4, CD25, and a transcription factor Foxp3, are mostly thymus derived and account for 5% to 10% of the circulating CD4+ T cell population. The cytokine interleukin-2 can stimulate their expansion but is not selective. In this article we present a novel way to chronically expand this T cell population, by complexing the cytokine interleukin-2 with an interleukin-2–neutralizing antibody, which prolongs its duration of action and specifically targets regulatory T cells, markedly increasing their numbers. Other interleukin-2–responsive lymphocytes that can accelerate atherosclerosis do not respond to this cytokine–antibody complex. Treatment with this cytokine–antibody complex inhibits the activation and proliferation of proatherogenic T cells and markedly attenuates development and progression of already developed atherosclerosis. From a clinical perspective, this finding is important because it demonstrates that by specifically targeting cells that can protect against atherosclerosis, in this case pharmacologically expanding regulatory T cell numbers, it is possible to suppress inflammation associated with atherosclerosis as well as attenuate progression of disease.
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- Cytokine Therapy With Interleukin-2/Anti–Interleukin-2 Monoclonal Antibody Complexes Expands CD4+CD25+Foxp3+ Regulatory T Cells and Attenuates Development and Progression of AtherosclerosisClinical PerspectiveTam Nguyen Dinh, Tin Soe Kyaw, Peter Kanellakis, Kelly To, Peter Tipping, Ban-Hock Toh, Alexander Bobik and Alex AgrotisCirculation. 2012;126:1256-1266, originally published September 4, 2012https://doi.org/10.1161/CIRCULATIONAHA.112.099044
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- Cytokine Therapy With Interleukin-2/Anti–Interleukin-2 Monoclonal Antibody Complexes Expands CD4+CD25+Foxp3+ Regulatory T Cells and Attenuates Development and Progression of AtherosclerosisClinical PerspectiveTam Nguyen Dinh, Tin Soe Kyaw, Peter Kanellakis, Kelly To, Peter Tipping, Ban-Hock Toh, Alexander Bobik and Alex AgrotisCirculation. 2012;126:1256-1266, originally published September 4, 2012https://doi.org/10.1161/CIRCULATIONAHA.112.099044


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Mayme J. Peranich, 95, formerly of Ely, dies
Mayme J. Peranich, 95, died on Friday, December 15, 2017 at Stoney River Memory Care, Marshfield.
Funeral services will be held at 4:00 pm on Wednesday, January 3, 2018 at Rembs Funeral Home, Marshfield, where the visitation will be from 2:00 pm on Wednesday until service time.
Mayme was born on August 17, 1922 in Ely, Minnesota to John F. and Angela (Kavschek) Germek and was a graduate of Ely High School. She was united in marriage to John S. Peranich in 1942 in Milwaukee. He died on May 11, 2012.
Shortly after their marriage Mayme and her husband lived in New Berlin where they resided until retirement. They then returned to Ely and resided on Burntside Lake where they enjoyed the outdoors being on the water, fishing, and mom also loved to entertain with family and friends. Mayme also liked traveling with John and they frequented the Caribbean, enjoying scuba diving.
She is survived by her son, Gene J. Peranich of Fayettville, AR and her daughter, Gayl S. Lick of Neillsville. She is also survived by two grandchildren, Dan A. Peranich and Theresa Peranich and a brother, Felix Germek of Milwaukee.
She was preceded in death by her parents, her husband and six siblings.
Condolences may be sent online to www.rembsfh.com


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Tales of a waitress who escaped the restaurant industry and then discovered a desk job kind of blows - so I put the apron back on. And I deliver pizza because getting paid to drive around listening to music is pretty awesome.
Tuesday, November 17, 2009
See this? It's called a MENU.
Then he'd better go somewhere else because a) we don't serve half sandwiches at six at night b) we don't have cups of soup and c) we don't have ham sandwiches at all.
Maybe if any of the three people at the table had even opened their menus, they might have known that
10 comments:
- Alyssa said...
what type of moron orders HALF a sandwich???!!!!!!!
- November 17, 2009 at 2:43 AM
- purplegirl said...
An old toothless one. :)
- November 17, 2009 at 3:35 AM
- Hellraiser said...
Just like when the menu changes...."We came in last week and had that dish", "Yes Sir, but the menu has changed since you were last here", "Oh! Can we still have that dish though?" NO YOU FUCKING CAN NOT, WE ARE NOT A FUCKING SUPERMARKET, hehe! :)
- November 17, 2009 at 5:11 AM
- The Veteran Server said...
Ah yes, love,love,LOVE the customers who create their own menu!! Lol! Your site is always so funny. God bless.
- November 17, 2009 at 6:32 AM
- megaphon said...
oh yessss... happens ALL the time! I just don't get it. And it never fails to make me REALLY angry!fulownsc
- November 17, 2009 at 7:18 AM
- megaphon said...
ooops... sorry, din't mean to post the captcha...! ^^
- November 17, 2009 at 7:19 AM
- HG Brit said...
This is my biggest restaurant pet peeve EVER. I hate it when people order things that aren't on the menu. It's on the table for a reason! I had a guy do that to me once, and he had more time than anyone to look at the menu because he was waiting on the rest of his group. And he even added that he "hope[d] it was still on the menu". UGH.
- November 17, 2009 at 7:47 AM
- Organic Meatbag said...
heheheeh, they might as well have asked for a tongue sandwich and a cup of gruel...
- November 17, 2009 at 8:29 AM
- SkippyMom said...
I don't know why - but my god this made me laugh - a?b?c? No ham sandwiches - open the menu you FREAKS.
I swear I would've stood, mute, in front of them until they opened the menus up.
Or brought out a cup of coffee along with an empty plate and when they asked why the plate? I would've replied "That is your imaginary ham sandwich - that is on YOUR imaginary menu. Unfortunately it isn't on real menu. You want real food read the REAL MENU."
GTFO. Heehee...love it!
- November 17, 2009 at 12:20 PM
- purplegirl said...
You all cranky me up. :)
- November 18, 2009 at 12:16 AM


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Article: We have a Business Continuity Plan – we're all done, right?
Linda Dickey, Partner
4Sight Group LLC
Just when you thought you were finished with your Business Continuity Plan "BCP" (also called Disaster Recovery Plan), the real work begins. The primary advantage of completing a BCP is that it helps you identify weaknesses in your organization that can be corrected before a disaster or disruption occurs.
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Back in Texas where we used to live, it became a habit to me to locks our doors for safety reason. I have many crimes are committed during day time where people are at work. I wasn't working before. I just stayed home with my son. Because of force of habit, there few times where I locked myself out, so my son and I couldn't get inside. Good thing we have a friend who lives nearby, so just went to her house, called my husband on the phone. The good thing about it, was his workplace was just 15 minutes away from home. He can go home and unlock the key. What if he work hours away? What would I do? Because of that experienced, we decided to put a spare key outside where it is safe. Something like a little safe for spare key. Anyway, if for some reason same thing happened to you especially if you live in Arizona, particularly in Scottsdale area, Scottsdale Locksmith provides an excellent service and quality service to their clients for a very reasonable price. They have experienced workers who do their job very well. You will be satisfied with every locksmith service they provide. Plus, they have been in business since 1971, owned and operated by a family, offers full service 24 hours a day 7 days a week; they are expert in opening safes, opening home locks, opening car locks, electric locks, and repair locks. So, for all your locks need, Scottsdale Locksmith is just a mouse or phone away! Visit their site now for more information.
Saturday, May 23, 2009
Scottsdale Locksmith , Best in Arizona
Posted by Cecile at Saturday, May 23, 2009
Labels: locksmith
1 comment:
- DogLover said...
A Bark Collar is a safe, effective and humane way to train your dog not to bark too much.
Clinical studies conducted by the College of Veterinary medicine of Cornell University have proven that Spray Bark Collars are TWICE as effective as shock collars! Sonic dog barking collars are virtually ineffective compared to spray.
- May 26, 2009 at 7:46 AM
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Although two of the women in this group wore different gowns at last nights Presentation Show, I'm not changing the picture here, in the interest of continuity.
Click here to view more poses, scroll below to vote for the woman who wowed you and scroll to the next post to see and vote for the gown changes!
Mississippi-----*----- Missouri
Montana -----*----- Nebraska
Nevada -----*----- New Hampshire
Vote below for your favorite gown presentation
among this group.
Click here to open the poll in its own page.
Scroll below for the first twenty contestants.


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Thursday, September 17, 2009
I'm on a diet so it is faux chocolate for me!
It's my birthday, but no cake for me! I am going to Paris on October 29th, so I need to lose 10 pounds. I'm sticking to faux chocolate! Let me share the recipe with you!
I used my distressing kit for this card! It is so handy for making faux chocolate. If you don't have a distressing kit yet, I know where you can get one. (Smiles). I'll put a link at the bottom of this post!
I used the following tools from the kit:
Paint Brush, White Magnolia Delta Paint, Cocoa Reinker. You will also need liquid glass.
Here is the recipe:
1. Take your paint tray from the distressing kit. Mix Liquid Glass, White Delta Paint and Chocolate Re-inker on the tray.
2. Stamp your image.
3. Paint the part of the stamp that you want to look like chocolate!
Fun, fun, fun! What a great way to add dimension to your artwork!
View or order distressing kit here!
Paper Used: Good Times- Level 2
Stamp Set- August Stamp Of The Month- It is your Day (last month's SOM but I have a few left- just email me if you are interested)
Inks: Sweet Leaf, Twilight and Buttercup
Posted by Tina Sutton- Missgingerdots at 6:38 AM
Labels: Cards, Project Instructions, Technique
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text: today introduced what it is billing as the world's first single-chip IEEE 802.11a/b/g wireless LAN chipset. The AR5006X chip integrates a media access controller, baseband processor and radio capable of operating at both 2.4 GHz and 5 GHz in a low-cost CMOS design, the company said. It also supports the IEEE 802.11i security standard and has an AES encryption engine built into its hardware, the company said.


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Powder Room 4
Mimi ON Jul 07, 2010 AT 2:32 pm
Aveda Scalp Remedy Dandruff Solution
This leave-in treatment uses botanical extracts to dramatically reduce flaking while calming itchy scalps and exfoliating skin. The list of ingredients includes fragrant rosemary and sage and soothing Echinacea, which works wonders on irritated skin.
£17, www.aveda.co.uk
Click here for the next product…


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SES now has room to move
THE revamp of the Coffs Harbour City SES headquarters is a fitting reward for our long-suffering rescuers.
For years they've endured cramped conditions at their Park Ave base but the extensions give them more room to move for training and emergency operations.
Local SES controller Bob White says the hard-won improvements took 18 years to achieve and he won't rest until a new and even better HQ takes shape.
"I'm very pleased to have reached this point. You think how long it's been going on for to finally get here," Mr White said at Tuesday night's official opening.
"Unfortunately it is still not enough. This unit desperately needs a modern, up-to-date headquarters with an emergency operations centre on a site large enough to operate far more efficiently and effectively than it does now.
"The City of Coffs Harbour needs the SES. In the past 14 years, this city has suffered two major floods and many smaller ones, a mini-cyclone, innumerable storms and we have even recorded tsunamis in the harbour."
The SES and the city council shared the $300,000 cost of the new training room, affectionately termed 'Bob's Baby' because of Mr White's relentless lobbying to bring it to fruition.
The facility was filled on Tuesday night with top brass and volunteers from all local and regional emergency services who work so closely during catastrophes.
SES Commissioner Murray Kear told the crowd SES units were more about the people who worked there than bricks and mortar were.
"We are people who meet community members on the worst days of their lives, so we need good facilities for training," Mr Kear said.
He presented long service awards to unit members Noel Furness (20 years), Wally Mulholland (20 years) and Ian Christensen (30 years), and recognised the world-class achievements of the local team which competed at the recent World Rescue Challenge/World Trauma Challenge/Australasian Road Rescue Organisation Challenge.
Mr White and his troops will now continue their push for a new HQ, no matter how long it takes.
While the city council has set aside land off Coramba Rd for the new site, Mr White says the ideal location would be on the corner of Beryl St and the Pacific Hwy if that's where a new police station and courthouse is to eventually go.


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Good morning.
The salon has been very busy this week, and I'm working late today and tomorrow.
The weather has been mild but wet, and it looks to be staying that way through the weekend.
My back appears to be stuck in the same place, despite going in for a massage every other day. I'm good during the day, sleep pretty good at night, but awake with a tight stiff, sore, back that requires moist heat to get flexible again. It appears I'll have to break down, spend the big bucks, and go to physical therapy. I'll finish the week and decide on Monday.
Our new neighbors at work have been busy redecorating their space. They've painted, put in a wood laminate floor, and are currently adding counters and cabinets. The owner came over and said she will be having an open house on the 15th, with a grand opening on the 24th. Will be interesting to check it out.
And with that, off to work!
Have a great weekend!


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FORT FRANCES, Ont. – Dryden GM Ice Dogs defenceman Cory Dennis had a pair of first period markers and an assist on the game-winner as they held on to defeat the Fort Frances Lakers 5-4 Saturday night in Superior International Junior Hockey League play at Ice for Kids Arena.
After Dennis gave Dryden the two-tally cushion heading into the second stanza, teammate Joseph Santalucia's first SIJHL goal upped the visitors lead to three at 5:08.
Special teams helped Fort Frances get back into it later in the frame as Ian Tookenay scored on the power play before adding another with the Lakers shorthanded to make it 3-2 after two.
The GM Ice Dogs regained their momentum with markers 52 seconds apart from Malcolm Huemmert and Eric Stout to go up 5-2 with 7:19 to play.
Battling back, Fort Frances connected with offensive efforts in rapid succession from Jaedin Ness, with his 10th, and Noah Loveday's seventh with over four minutes left in regulation.
However Dryden tightened up defensively from there as they held on for the road win.
The GM Ice Dogs outshot the Lakers 53-31 in the match-up with Jacob Gnidziejko picking up the win and Ismael Ralsten the defeat.


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Carmela Nepomuceno
Born in 1995; Auckland-based artist, Carmela Nepomuceno, works with themes of faith and identity. Carmela's take on Sacred Art led her to delve into the significance of the body and hand gestures.
Since completing her Bachelor's Degree in Fine Arts at the University of Auckland (Elam), Carmela continues to explore her multidisciplinary practice and skills through sculpture, printmaking, installation and drawing. In her constant idea of a 'gift of self,' she invites and hopes that her work draws others to a deeper ponder and witness.


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This post is from Marsha, author of A House Full of Health, who has been a previous contributor to VBU! You can read her previous posts here and here.
Marsha wrote a preface and I think it's important, here she is in her own words, "I wrote this blog 6 months post making one of the greatest decisions of my life. I'm new to the vegan world and to the blogging world so I know what it's like to be nervous about putting yourself out there to try new things. I want to share with others what it's like to go vegan. Blogging, well I still don't quite have the hang of that so can't help there. The vegan world, I've found, is so friendly, I've been so grateful for all the wonderful support. Cheers and happy reading!"
You can find Marsha on Twitter, Facebook and through her blog. Please welcome back Marsha!
Going vegan! Who's going vegan? I am? Really? Are you sure?
Oh how I thought I was being incredibly crazy! I thought the fact that the hubby mentioned first meant something infiltrated our water system affecting our brains!!
It's been 6 months!
And, now? Nope, I don't think we are crazy! We are so very happy with our choice!
Have you thought about the vegan/vegetarian lifestyle? Does it intimidate you just a bit? It did for me, actually. I remember the very first week we didn't eat any meat or dairy I thought I would eat the house! I was SO hungry! I'd wake up in the middle of the night starving! Famished! Ready to eat the cats as they lie sleeping on my bed! Anything! I was so hungry!
Think about the times you eat Chinese food, within the hour you are hungry again. It's as if you didn't eat any dinner, right? That's how I felt!
Then, I thought, what was I missing when eating a big plate of Chinese food? Protein. I was missing protein. I generally ordered a plate full of veggies in some sort of sauce over a bed of white rice. Of course I was hungry an hour later. That didn't have the amount of protein I was used to as I sat down to a big steak!
Just like everyone else, we vegans need complete protein to get all our essential amino acids. We don't want to fall prey to the negative protein balance now do we? That's starvation and I love food so that is NOT an option.
According to the USDA, a man age 40 weighing 160 pounds needs 56 g of protein per day. A woman, weighing 140 pounds age 40 needs 46 g of protein per day. That's about 0.36 g of protein for every pound of body weight.
Protein needs to very based on age, size, health, physical activity, body weight, body type and for those pregnant or nursing.
When I speak of protein, I speak of a complete protein, all essential amino acids needed. There are some plant-based foods that carry all essential amino acids. Soy is one of them as is quinoa. It was once believed that vegetarians and vegans could possibly develop a protein deficiency if they didn't get all the essential amino acids that they needed within one meal. But, when we eat, our body stores amino acids in something like a storage bank, we use what we need when we need it. So, it's not a necessity to get all 8 amino acids in one sitting. As long as you eat a variety of foods throughout the day you will get all of the essential amino acids that you need. So, don't go eat a block of tofu. It's just gross.
Okay, so now that I have protein in my system I don't wake up wanting to eat everything in sight in the middle of the night. It took about a week and I was used to being "vegan." Ah! Much better!
What is vegan?
Vegan has so many different definitions. Some are vegan based on ethical reasons, some for environmental reasons, some for health reasons. There are vegans that combine all of them as well. It's up to you to decide what is best for you. I don't judge how vegan other people are as I only wish that people don't judge me.
Being vegan, for myself, is mostly about health. I am more aware of items that I purchase, trying to purchase cruelty free items, but I'm not perfect. And, because I am not rich (although I wish that I were!) I still own a variety of leather shoes. Does this make me a bad vegan? To some, it may. For me, I certainly don't want to waste what I already own. But, I will be more aware next time I buy a pair of shoes.
I was definitely pretty nervous when I decided to stop eating meat and dairy. I didn't know what to do. I was trained in a French culinary school, I cooked with meat and butter, lots of it. That's when I decided to start this blog. And then I found twitter, I know, that sounds so weird, right? But really I couldn't have done it without all my awesome vegan Twitter friends.
Twitter is where I met Dreena Burton. I must say, Twitter and blogging are like high school. You want to fit in, you want to be the cool kid, and you want to be noticed. Dreena was like my high school crush, in a way. She's the cool kid and I wanted her to notice me. She was the first that gave me the advice to keep on tweeting and keep on blogging. Then, one day she followed me on twitter. I almost peed my pants. Seriously! The cool kid followed me! But, to be serious Dreena is an amazing vegan cookbook author. I ordered her book, Let Them Eat Vegan, off of Amazon and waited, patiently, for it to arrive. What I love about Dreena's book, is the simplicity of all the recipes, they use of whole foods, and all of the real life suggestions to make things just a little bit easier. Read my view review here. Anyway, I don't think I could've been vegan very long without Let Them Eat Vegan. I thought being vegan meant lots and lots of fake meats. I am not a huge fan of fake meats! Being vegan absolutely does not mean you need to stuff your faces with the fake stuff!
I have met many supportive people on Twitter, Facebook, and around town. Support is key.
I met another friend on twitter, Janae Wise, mother of 4, vegan, gluten-free, fitness instructor, and all around wonderful woman. She is constantly encouraging my blogging by reminding me that I am new. All bloggers have to start somewhere and I can't be perfect or the best right off the bat. She is so right. She is very encouraging and has kept me going. One day I strive to be a wonderful picture taker, blogger, and inspiration like she has shown to be herself.
Of course, I have a good friend that lives nearby that welcomes all my questions no matter how silly. Mel Mason, My holistic health coach, has been a saint. Without her encouragement, dedication, and willingness to listen and help without judgment I would not have made it as far as I have.
How do you go vegan or vegetarian you ask? Yes, it will take a little dedication, practice, and time. But, it is all worth it. Your heart, health, and happiness will thank you.
I am a lover of lists. I make lists for what needs to be done during the day, what I need to pack for trips, what food I'm going to make during the week, and a list of items I need to buy at the grocery store. This makes the process that much easier.
So, here are a couple of lists. What I've learned, what's in my pantry, what's in my refrigerator, and some of my favorite vegan staples and some of yours.
A couple of things that I've learned in the past 6 months:
Have support: family, friends, Twitter, Facebook
Enjoy your food
Have a well-stocked pantry
Plan your meals
Ask questions, of me, of dietitians, nutritionists, healthcare providers, friends
Talk to people
Don't give up
Staples I like to keep in my pantry:
Rice, brown and white. Forbidden rice as well.
Canned tomatoes
Beans, all varieties (kidney beans, black beans, garbanzo beans, white beans, any bean!). Dried and canned
Vegetable stock
Better than bullion vegetable base
Green chilies, Trader Joe's has the best!
Black olives
Artichoke hearts
Sun-dried tomatoes
Pitted dates
Spelt flour
Chia seeds, great information on Chia seeds found here
Raw cashews, other raw nuts and seeds
Raw sugar
Agave Nectar
Bragg's Liquid Amino Acids
Popcorn kernels
|One of my favorites! Popcorn!
Vegetables and fruit I always have on hand:
Lettuce, Romain
Sugar snap peas
Purple cabbage
Lemon and limes
Onions, red and yellow
Mini bell peppers
|Apples, Oranges, lemons!
Items I like to have on hand, in my fridge:
Almond milk or other plant-based milks
Hummus, different varieties
Hemp seeds
Nondairy plain yogurt
Flax seed oil
Hemp seed oil
Maple syrup
Tofu, I know tofu can be scary! But, give it a try! There are so many ways to use it and love it!
The first time you go grocery shopping it may cost you a little bit more than your usual grocery trip. But, this is just to stock your shelves with all your staples. Once you get the hang of it, shopping and cost will be easy and low. I tend to shop weekly because all that produce barely fits in my refrigerator each week. If I shopped bi-weekly there would be no way. I even created extra space, a sort of pantry, in my laundry room to make room for all the goodness I buy.
|Raw Cashews
My favorite staples:
Raw cashews
Braggs Liquid Amino Acids
Carrots, celery, onion (I lump these together because they often go together in soups and such)
Hemp seeds
Chia seeds
|Chia Seeds
I asked of you some of your favorite staples in your vegan kitchen:
Quinoa of course was one of them. Tofu in tempeh, kale and other leafy greens, nutritional yeast, beans, raw cashews, and flax oil.
Do you have any others to add to my list?
I believe a good diet, is one that's not a diet at all. More of a lifestyle. You eat healthy because you want to be healthy. You are what you eat, as they say. Going vegan can be intimidating, difficult, expensive. But, as with any new thing this is only the beginning. Once you're in, you're in. Less difficult, less intimidating, and less expensive. I've been at this for 6 months and I sometimes feel like I've been doing it my whole life. I'm still learning how to cook, how to use items I've never heard of, and what substitutes I need to use for certain things. But I love the learning, the experimenting, and the vegan world that I've fallen into. Everyone has been so supportive and helpful.
Go out, give it a try, experiment, have fun with it! If you have questions drop me a line! I'd love to hear from you. I'm here to be supportive.
Blogging, vegan, it's all new to me but I'm here to stay.
Cheers and happy eating!
Combichrist – Caliber:Death
Peter Murphy – Cuts You Up
The Faint – Fish in a Womb
Justin Timberlake – Rock Your Body
Accersory – Matrix


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Spoiler alert: Don't click the video clip embedded above if you need to remain in the dark about how this year's Emmy-nominated shows finished their seasons. However, if you don't mind gorging on spoilers and want to watch a couple of guys spewing non-stop for six minutes, take a deep breath and check out the Fine Brothers' clip in which they recap finales for 100 hit TV shows.
In advance of Sunday's Emmy Awards broadcast (CBS, 8 p.m. EST, delayed on the West Coast), the video kicks off with the big reveals from last season for Best Drama contenders, including Lost and Dexter. Ben and Rafi Fine then spoil Best Comedy nominees 30 Rock, Entourage, Family Guy, Flight of the Conchords and The Office before racing through another seven dozen classic TV finales.
A few minutes into the blitz, Fafi Fine steps out of just-the-facts mode to lash out at Winnie Cooper (played by math genius Danica McKellar) in The Wonder Years, blaming the character for ruining his adolescence. The brothers also single out 7th Heaven as a series that sounds "horrible."
The clip's own finale? A blackout, a homage to The Soprano's whacked out closing shot.
Follow us on Twitter: @hughhart and @theunderwire.
See Also:
- 7 Webotainers Worth Watching
- Spärhusen Is Swedish for Spinal Tap
- Gervais, Merchant Bury Schindler's, Fiennes in Cemetery
- Spoiler Wars Heat Up as Lost Returns


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Recent sumo wrestling topics, Yokozuna Hakuho (Mongolian origin) consecutive winning record ...
The roots of sumo seem to be in ancient Israel.
There are three major fathers of the Israeli ethnic group (Sufarady Jewish in the Bible), Abraham, Isaac, and Jacob, but the event that God gave Israel from God is the era of Jacob Fathers.
It is written in the Bible that Jacob fought (sumo) with the gods of God. At that time Jacob got the name Israel from God.
Jacob remained after her independence. At that time, someone fought with Jacob until dawn.
However, he saw that he could not overcome Jacob and hit Jacob's thigh joints, so the joints of the thighs went out while fighting.
Let me go away. Jacob answered, because the night was breaking, he said.
No, I will not let it go until I bless you.
That man asked what he would say, and the man answered and said Jacob, he said.
Your name is not Jacob anymore, from now on it is called Israel. Because you fought with God and won. (Genesis 32: 23-29)
The event that this Jacob grappled with the God of Faith (sumo wrestling) is very important for the Israeli people.
From that time on, the name Israel and the blessing from God were given.
It is the reason why the Israeli people who are descendants of Jacob are said to be God's electmen because they won sumo wrestlers.
Sumo is an important historical fact for the Jewish people, it is Jew who constitutes the center of the Japanese nation today. Sumo is still present as a proof of the historical fact now.
The Emperor of Japan is said to be a bloodline Levi (priest) written in the Old Testament, and it seems to be said that Levi is the highest leader.
It is said that religious acts in this Old Testament changed to Shinto of Japan.
It is said that Emperor Showa was very much concerned with the cow's thigh meat. It may have been recognized that the Emperor, the Imperial Family, received the flow of priests.
One of the dedication to this emperor, the Imperial family (Shinto) is sumo.
Also in the Old Testament, the place of sumo is marked.
Sumo is Japanese traditional Shinto, national sports from ancient times.
Sumo is also a noble Shinto shrine that dedicates to the Emperor, the Imperial family.
Sumo is also one of the few samurai wrests left in contemporary Japan.
It does not seem to be dedicating to the Emperor in the sumo wins or losses.
I think that it is impossible for a sumo wrestler other than Japanese to understand this sumo wrestling mind, shrine, Shintoism, dedication to the Imperial family.
Since ancient times Japanese people have used the Emperor, the Imperial family as a center of mind.
The Japanese imperial family has a history of thousands of years without everlasting worldwide.
From the moment the Japanese was born, it is said that, as a result, the principal becomes a member of Shinto even if he / she is unconscious.
Because other than Japanese can not enter Shinto, it is said that Japanese Shintoism is a religion discriminating foreigners.
Located at the top of this Shinto is the Emperor.
As a priest, the Emperor seems to be a big daily routine to pray everyday in the form of a Japanese state body, the stability of the people in the form of a Shinto.
Sumo may inform us of the identity as a Japanese..


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..but my motivation has been low this last week. I'm in serious need of another holiday.
All I've done this last week is watched imported American TV. I have a new favourite. It's called Burn Notice and I'm not sure if it has aired or even will air in the UK. It's about a spy, Michael Westen, who's been 'burned' by his employers at Langley. That's to say he's been fired and cut off, and they could well be trying to kill him. Whilst trying to get to the bottom of this back story, he has to earn a crust, which he does by helping the citizens of Miami with their problems. it's all very undemanding and jokey. Pitched somewhere around Moonlighting, Magnum and The A Team, with a maybe a flavour of Christopher Nolan's Memento. It has Jeffrey Donavan in the lead, with solid support from Gabrielle Anwar (dubious Irish accent though), Sharon Gless and the peerless Bruce 'If Chins Could Kill' Campbell as his burned out sidekick.
Anyway, I liked it and watched the whole first season in the last week.
As all I've been able to muster is a very short 'badly written TV review' this week, here's a picture of a dog.


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Jan 13 2013
Social Media Step 1: a LinkedIn profile
Before you start with Twitter, Facebook, Google+ or any other social media marketing for your business, make sure you have an up to date LinkedIn profile. This will ensure that other professionals can find you online, and you have control over what you want them to know about you as a professional.
The three main reasons why every professional person should have a LinkedIn profile:
- Search Results
If you have never 'Googled' your own name, do it now! If you have a LinkedIn profile, your LinkedIn profile will most likely be the first search result. Now picture someone who doesn't know much about you, but would like to do business with you… what will they find when they Google your name? It's even more important if you have namesakes because your profile on LinkedIn should make it easy to figure out which one YOU are!
- You have control
YOU complete your profile, adding everything you want other professionals to know about you. This allows you to decide whether your time as a barman on Ibiza 15 years ago contributes to who you are as a professional today, or whether you prefer to relate that experience on another platform. Your LinkedIn profile should include your work experience, skills, education and a summary about you. While your LinkedIn profile is NOT a CV, you should be honest and thorough in completing it but keeping it professional. Think about the fact that other professionals will be using this information to decide whether you have the skills and experience for the services they expect from you or your business.
- LinkedIn is a PROFESSIONAL network
Your holiday snaps, jokes you find funny, and your sometimes crazy friends will not be part of your LinkedIn profile, but would rather be found on Facebook which is a personal network. Keep your personal and professional networks separate and use them appropriately. A potential client or employer wants to know about you as a professional!
You should have only ONE LinkedIn profile, not a new one every time you change careers or jobs. Login today and make sure your profile is up to date, with a recognisable photo of you, and your current position as a headline.