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Vibrio fischeri NEU2011
A page on the microorganism Vibrio fischeri
[[File:|400px|thumb|right|Figure 1:]]
Higher order taxa
Vibrio fischeri
- Kingdom: Bacteria
- Phylum: Proteobacteria
- Class: Gamma Proteobacteria
- Order: Vibrionales
- Family: Vibrionaceae
- Genus: Vibrio
- Species: V. fischeri
Description and Significance
Vibrio Fischeri is a member of the phylum proteobacteria. These bacteria are motile, gram-negative rods that are found in temperate and subtropical waters. These heterotrophic bacteria use flagella as a means of movement and they are most famous for their bioluminescence properties. This bioluminescence can be seen in Figure 1. V. Fischeri is mainly found living in symbiosis with various deep sea marine animals such as monocentrid fishes and bobtail squid. It can be found within unique light-organs or as part of the normal gut of these marine animals. This bacteria can also be found living as free bacteria in small quantities surviving on decaying organic matter (11).
V. Fischeri use proteins coded by a set of genes called the lux operon to produce bioluminescence. The light is produced in a chemical reaction where luciferin is oxidized by the enzyme luciferase. As a result of the oxidation, a blue-green light is emitted. The symbiotic relationship between a strain of the V. Fischeri and its host, the bobtail squid Euprymna scolopesseen in Figure 2, has been studied extensively. The bobtail squid acquires these bacteria from its surroundings and uses it as a protection from predators. Within several hours of being ingested, these bacteria begin to change. They decrease in size, lose their flagella, and begin to emit light. These bacteria help to eliminate the squid's shadow caused by the moonlight above (13).
The isolation and cloning of the lux gene from V. Fischeri, and their use as a reporter gene, have provided scientists with many valuable research techniques. The lux gene has enabled scientists to visually study and examine many living organisms at a cellular level. Likewise, V. Fischeri cells have been made commercially available to ecotoxicologists to detect contaminants in the environment more quickly and cheaper than most other available methods (7). V. fischeri is also the bacterium in which acyl-homoserine lactone, quorum sensing, was first discovered and described. Quorum sensing is a process that allows the bacteria to coordinate their gene expression according to the density of the population. In V. fischeri, when the bacteria have become highly concentrated in the light organs of the bobtail squid, luciferase is produced leading to bioluminescence. The discovery of quorum sensing has lead to the disclosure of many other bacteria that use this type of cell coordination process. Some bacteria such as Escherichia coli, Salmonella enterica, and Pseudomonas aeruginosa use quorum sensing (2). In addition to being the pioneering bacteria in the discovery of quorum sensing, quorum sensing in V. fischeri has lead many new research opportunities in targeting and combating bacterial infections and also has begun to be used in research to discover a way to fight cancerous tumors (1).
Genome Structure
The genome of V.fisheri contains 4273718 nucleotides, 3817 protein genes and 165 RNA genes. The chromosomes I and II are circular. The sequence of the chromosome 1 is NC_006840 and its lenght is equal to 2897536. The sequence of the chromosome II is NC_006842 and its lenght is equal to 1330333. The ES114 strain is characterized by a circular plasmid known as pES100. The number of taxonomy of the Vibrio fisheri is 312309 (KEGG).
Past studies have demonstrated that Vibrio fischeri is successful at adapting to a variety of ecological niches. They are commonly present as planktonic cells in seawater or sediments, and as saprophytes. V. fischeri are usually found in very low quantities in almost all oceans of the world, preferentially found in temperate and sub-tropical waters. They populate fecal materials. In higher concentration, V. fischeri have cooperative associations with certain deep sea marine life within special light-organs. Indeed, they can form associations with specialized symbiotic organs of marine squids and fishes (Nealson).
Although many studies have reported specific niches and predictable patterns in geographical distribution for luminous bacterial species the factors underlying their ecological dynamics is not well understood yet (Feldman). Additionally the bacteria can be pathogenic to certain species of marine invertebrates, some of which are commercially farmed in aquaculture. This disease is known as luminous vibriosis (Orndorff).
Cell Structure and Metabolism
Vibrio fischeri is a Gram-negative bioluminescent oxidase-positive marine bacterium composed of a cell wall with an outer membrane consisting of inner cytoplasmic membrane covered by peptidoglycan layer followed by periplasmic space to the lipopolysaccharides. They are straight rod with 0.8-1.3 um in diameter and 1.8-2.4um in length. The bacteria has 1-3 polar unsheathed flagella.
This bacterium is capable of respiratory and fermentative metabolism because it is a chemoorganotroph. Vibrio fischeri is a facultative anaerobe. It cannot dentrify, implying that it cannot use nitrogen in its metabolism.
The bacteria form mutually symbiotic relationships with various species of fish and squids with their ability to produce bioluminescence. The production of bioluminescence is enabled by the proteins that are encoded in a set of genes referred to as the lux operon. Light is produced when an enzyme, luciferase, oxidizes luciferin, a substrate molecule. This reaction releases free energy in the form of blue-green light at 480nm-490nm(blue-green). The reaction that occurs in Vibrio fischeri is as follows:
FMNH2 + RCHO + O2 --> FMN + RCOOH + H2O + hv(490nm)
The bioluminescence quantum yield has been estimated to be 10-30% according to Encyclopedia of Life Sciences (Davis, Aubrey).
Vibrio fischeri itself is non-pathogenic and is classified as a mutualistic symbiont. The V. fischeri species is known for its beneficial association with the light organ of the bobtail squid, Euprymna scolopes (14).
In the genus of Vibrio, are several dozen species known to participate in a diversity of pathogenic interactions with other organisms. Among these species are Vibrio cholerae, shown in Figure 3, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio harveyi. V. cholerae, V. parahaemolyticus, and V. vulnificus are best known are causing illness in humans. Vibrio infections are characterized in humans as a foodborne illness. This would be due to the consumption of contaminated seafood or the exposure of an open wound to tainted seawater. Symptoms present themselves as gastroenteritis issues such as diarrhea, nausea, vomiting, and headaches. Infections due to open wound sites would include swelling at the site, pain, erythema, and necrosis. In addition to these ailments,V. vulnificus is also responsible for causing septicemia in an infected individual. Of the pathogenic species present in humans, V. vulnificus is linked with the highest fatality rate (6).
Vibrio harveyi is pathogenic to certain species of marine invertebrates and is known as luminous vibriosis. Luminous vibriosis can be found in sharks, seabass, seahorses, lobster, and shrimp. This disease forms plaques in the mouth, innards, and appendages. This causes the loss of limb function and soon after death of the organism. Luminous vibriosis is the leading cause of death for commercially farmed shrimp and other aquaculture (12).
Current Research
Vibrio fischeri has been the subject of extensive studies in physiology, biochemistry, and genetics that underlie the mechanics of bacterial bioluminescence. Past studies of V. fischeri lead to the discovery of a signaling behavior termed quorum sensing (2).
In a recent study, Environmentally Controlled Invasion of Cancer Cells by Engineered Bacteria, Anderson and his colleagues, Clarke, Arkin, and Voigt, were able to engineer bacteria that are able to target and then invade mammalian cancer cells. This is able to occur due to quorum sensing genes from the bacteria Vibrio fischeri. In this line of research, Anderson and his colleagues were able to engineer the interaction between bacteria and cancer cells to depend on heterologous environmental signals. This is made possible due to bacteria's ability to sense their environment and distinguish between different cell types. They used Escherichia coli to invade the cancer derived cells including HeLa, HepG2, and U2OS cells. Using the Vibrio fischeri lux quorum sensing circuit, the hypoxia-responsive fdhF promoter, or the arabinose-inducible araBAD promoter, researchers were able to environmentally restrict invasion of the bacteria into the cancer cells whose cells had densities greater than 10⁸ bacteria/ml. The quorum sensing genes used from V. fischeri triggered the invasion of a gene only if a cell had high numbers. The bacteria were also attracted to the cancer cells due to their hypoxic and nutrient rich microenvironments. Researchers hope that one day this finding could be used to engineer bacteria that sense the microenvironment of a tumor, invade it, and then release a cytotoxic agent that can destroy cancerous tumors (1).
Interesting Facts
Vibrio fischeri and its symbiont Euprymna scolopes,a squid, work together to survive, thrive, and successfully reproduce. Euprymna scolopes use the bacteria V. fischeri for its bioluminescence properties. The squid is a nocturnal feeder, and uses the light generated from V. fischeri to eliminate its shadow caused by the moonlight above. Every morning, the squid releases 90% of the V. fischeri population in its light organ back into the water. This is most likely to insure that new squid populations have the bacteria available to them (9).
The squid is able to control the brightness and the direction of their illuminations produced by V. fischeri. The light sensing organs in the Euprymna scolopes shown in Figure 4,also does much more than just produce light, they are also able to sense light. Due to proteins located in the light organs, these organs are an extra set of "primitive eyes" each with their own iris and lens (4).
- Anderson, J. C. "Environmentally Controlled Invasion of Cancer Cells by Engineered Bacteria."Science Direct. Elsevier, 14 Nov. 2005. Web. 2 Apr. 2011. http://www.voigtlab.ucsf.edu/papers/2006_anderson_jmb_invasion.pdf.
- Atkinson, Steve. "Quorum Sensing." Quorum Sensing. Nottingham University Biological Sciences. Web. 5 Mar. 2011. http://www.nottingham.ac.uk/quorum/index.htm.
- Davis, Aubrey. Biology (BIMM) 101 Lab Manual. p. ii. AS Soft Reserves, Winter 2007. Hoi Ho, Thong Huy Do, Tony Tran Ho, Derek Lee. "Vibrio infections." 2007 January. Emedicine Specialties. http://www.emedicine.com/med/topic2375.htm
- "Fascinating Bioluminescent Organisms." Amazing Facts. 22 Aug. 2009. Web. 02 Apr. 2011. http://myamazingfact.blogspot.com/2009/08/fascinating-bioluminescent-organisms.html.
- Feldman K A, Buck J D. Distribution and characterization of luminescent bacteria in a temperate estuary. Estuaries. 1984;7:93–97.
- Fox, Maggie. "Vibrio Cholerae." Online Textbook of Bacteriology. 09 Dec. 2004. Web. 05 Apr. 2011. http://www.textbookofbacteriology.net/cholera.html.
- KEGG. Vibrio Fischeri Genome Information. Web. 18 Feb. 2001. http://www.genome.jp/kegg-bin/show_organism?org=vfi.
- Koropatnick, Tanya. "Squid/Vibrio." SERC. Nov. 2006. Web. 21 Feb. 2011. http://serc.carleton.edu/microbelife/topics/marinesymbiosis/squid-vibrio/.
- Madigan M, Martinko J (editors) (2005). Brock Biology of Microorganisms (13th ed.). Prentice Hall.
- Maiden, Stephanie. "Vibrio Fischeri." Missouri University of Science and Technology. Web. 02 Apr. 2011. http://web.mst.edu/~microbio/BIO221_2004/V_fischeri.htm.
- "Micro-Discoveries Online - Germs That Infect Humans." Au Musée - Le Musée. Web. 05 Apr. 2011. http://www.musee-afrappier.qc.ca/en/index.php?pageid=3113b.
- Nealson KH, Hastings JW.PMID: 396467 [PubMed - indexed for MEDLINE]PMCID: PMC281490
- Nyholm, S. V. "Cover Photo — August 29, 2000, 97 (18)." Proceedings of the National Academy of Sciences. 29 Aug. 2000. Web. 05 Apr. 2011. http://www.pnas.org/content/97/18.cover-expansion.
- Orndorff S A, Colwell R R. Distribution and identification of luminous bacteria from the Sargasso Sea. Appl Environ Microbiol. 1980;39:983–987.
- "US NSF - Multimedia Gallery - Adult Squid Species Euprymna Scolopes." Nsf.gov - National Science Foundation - US National Science Foundation (NSF). 09 Mar. 2011. Web. 05 Mar. 2011.http://www.nsf.gov/news/mmg/mmg_disp.cfm?med_id=51886.
- "V. Fischeri." The University of Nottingham. Web. 13 Feb. 2011. http://www.nottingham.ac.uk/quorum/fischerimain.htm.
- "Vibrio Fischeri - Encyclopedia Article - Citizendium." Welcome to Citizendium - Citizendium. 16 Feb. 2010. Web. 27 Mar. 2011. http://en.citizendium.org/wiki/Vibrio_fischeri.
- "Vibrio Fischeri - Euprymna Scolopes Symbiosis." Integrated Genomics. Aug. 2007. Web. 14 Feb. 2011. http://web.uconn.edu/mcbstaff/graf/VfEs/VfEssym.htm.
- "Vibrio Fischeri." Missouri University of Science and Technology. Web. 05 Mar. 2011. http://web.mst.edu/~microbio/BIO221_2010/V_fischeri-2.html.


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Should greater restrictions be placed on elderly drivers essay driving is a simple undertaking that many of us do on a day-to-day footing most people are safe drivers while a few are incapable of driving safely. Value drivers create greater customer value marketing essay print reference this disclaimer: this work has been submitted by a student this is not an example of . Should greater restrictions be placed on elderly drivers as we grow older, our reflexes become slower and our minds become less sharp than they used to be. Should there be greater restrictions placed on high-speed police chases hello everyone my name is brandon and today i agree with this argument. I'm doing a persuasive essay on senior driving and why elders shouldn't be driving after the age of 75 involved elderly drivers with an age greater .
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Gang shootout at New Jersey arts festival kills 1, injures 22
Secret Service intervenes when CBS News attempts to ask Jared Kushner a questionOn a flight to New York Tuesday, CBS News correspondent Errol Barnett tried to ask President Trump's son-in-law and advisor Jared Kushner a question. However, Secret Service intervened.CBS News
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June 17 (Reuters) - A suspected gunman was shot dead and 22 people were injured on Sunday after a dispute among rival gangs erupted into gunfire at an all-night arts festival in Trenton, New Jersey, officials said.
One of the shooting victims was a 13-year-old boy who was in extremely critical condition, Mercer County Prosecutor Angelo Onofri told a news conference. Three others were in critical condition.
At least two people opened fire around 2:45 a.m. at the annual Art All Night event in Trenton, about 60 miles (100 km) southwest of New York City.
The dead suspect was identified by police as Tahaij Wells, 33, and another suspect, Amir Armstrong, 23, was in police custody.
Of the 22 people injured, 17 people of them were shot, Onofri said, and multiple weapons were recovered from the scene.
Officials believe the suspect was killed by police, and the case was being treated as an officer-involved shooting, Onofri said.
More than 1,000 people were believed to have been at the festival when the violence started.
"It absolutely could have been worse, given the confined space and the number of shots that appear to have been fired," Onofri told a news conference.
"The shooting appears to be related to several neighborhood gangs from here in the city of Trenton having a dispute at the venue," Onofri said.
Organizers canceled the remainder of the event, billed as "24 hours of community, creativity and inspiration."
The festival typically draws more than 30,000 visitors to view work from more than 1,500 artists as well as exhibitions of glass blowing and woodwork, The Trentonian reported on its website. About 50 bands also play on three stages.
"We're still processing much of this and we don't have many answers at this time but please know that our staff, our volunteers, our artists and musicians all seem to be healthy and accounted for," the organizers wrote on Facebook on Sunday. "Our sincere, heartfelt sympathies are with those who were injured."
The New Jersey shooting occurred amid a debate about U.S. gun laws that was given fresh impetus by the massacre in February of 17 people at a high school in Parkland, Florida.
"It is a fact that our cities as well as our suburbs throughout America are experiencing an increase in public shootings and public unrest such as this," Trenton Mayor Eric Jackson told the news conference. "This isn't just a random act of violence. This is a public health issue."
New Jersey Governor Phil Murphy spoke at a Trenton church on Sunday, noting that he signed six gun control bills into law on Wednesday but saying the proliferation of guns required a national solution.
"Congress needs to act," Murphy said. (Reporting by Daniel Wallis and Daniel Trotta in New York; Additional reporting by Rich McKay; Editing by Matthew Mpoke Bigg)
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International Seaways (INSW) Receives New Coverage from Analysts at Stifel Nicolaus
Stifel Nicolaus started coverage on shares of International Seaways (NYSE:INSW) in a research note released on Tuesday morning, MarketBeat Ratings reports. The firm issued a buy rating and a $25.00 price target on the transportation company's stock.
Separately, ValuEngine upgraded shares of International Seaways from a sell rating to a hold rating in a report on Saturday, June 2nd. Two investment analysts have rated the stock with a hold rating and three have assigned a buy rating to the company. The stock presently has a consensus rating of Buy and a consensus price target of $27.00.
INSW stock opened at $20.47 on Tuesday. The company has a quick ratio of 1.94, a current ratio of 1.99 and a debt-to-equity ratio of 0.75. International Seaways has a 52-week low of $15.23 and a 52-week high of $24.78.
In related news, Director Randee E. Day sold 4,000 shares of the stock in a transaction dated Monday, August 27th. The shares were sold at an average price of $20.95, for a total value of $83,800.00. The transaction was disclosed in a legal filing with the Securities & Exchange Commission, which is accessible through this hyperlink. Also, major shareholder Paulson & Co. Inc. sold 125,000 shares of the stock in a transaction dated Friday, September 14th. The shares were sold at an average price of $18.15, for a total transaction of $2,268,750.00. The disclosure for this sale can be found here. Insiders have sold 841,153 shares of company stock valued at $16,784,404 over the last 90 days. Corporate insiders own 0.90% of the company's stock.
Several hedge funds and other institutional investors have recently added to or reduced their stakes in INSW. Frontier Capital Management Co. LLC lifted its stake in shares of International Seaways by 49.8% in the second quarter. Frontier Capital Management Co. LLC now owns 1,288,592 shares of the transportation company's stock valued at $29,818,000 after buying an additional 428,321 shares during the period. Dimensional Fund Advisors LP lifted its stake in shares of International Seaways by 32.1% in the first quarter. Dimensional Fund Advisors LP now owns 1,030,466 shares of the transportation company's stock valued at $18,136,000 after buying an additional 250,173 shares during the period. Bienville Capital Management LLC bought a new stake in shares of International Seaways in the second quarter valued at about $1,391,000. Millennium Management LLC bought a new stake in shares of International Seaways in the second quarter valued at about $1,160,000. Finally, Paloma Partners Management Co bought a new stake in shares of International Seaways in the second quarter valued at about $956,000. Institutional investors and hedge funds own 85.39% of the company's stock.
About International Seaways
International Seaways, Inc owns and operates a fleet of oceangoing vessels for the transportation of crude oil and petroleum products in the International Flag trades. It operates through two segments, Crude Tankers and Product Carriers. As of June 14, 2018, the company owned and operated a fleet of 55 vessels, including 1 ultra large crude carrier, 14 very large crude carriers, 2 Suezmaxes, 7 Aframaxes, 12 Panamaxes, and 13 medium range tankers.
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Dot net projects names jobs
Create 70 conference name tags in PDF file for our company offsite. Will provide dimensions. Already have template in place.
We have a website which was previously developed in Dot Net. Now we have redeveloped the website using magento 2, and we want to migrate the same database. Motive is we want to have the client base and products and userbase as was in previous site. Let me know if this is something you can handle. Only professionals.
I WANTED TO SELL MY CUSTOMER DATA INCLUDING NAMES, ADDRESS, CELL NUMBER AND EMAIL. US CITIZENS. My website is a ecommerce website we are doing online trading.
Need software mini projects documentation in a predefined format for engineering students.
An excel spreadsheet will hold the existing names of the files and the names that they must be changed too. The information for each file is contained in its own cell. 9 examples shown below: MP-JP-115_1 ([เข้าสู่ระบบเพื่อดู URL]) E-VP-110-114_1 ([เข้าสู่ระบบเพื่อดู URL]) E-T1-380-23_1 ([เข้าสู่ระบบเพื่อดู URL]) E-T1-380-21_1 ([เข้าสู่ระบบเพื่อดู URL]) E-T1-380-22_1 ([เข้าสู่ระบบเพ...
I need someone to walk me through the URL harvesting from company names in a Google Spreadsheet without my having to send you the data. One approach is partially in this video but this may not be the right approach. Your assistance is greatly appreciated. You will be paid $10 following a successful walk-through
Dear Sir/Madam, I have another isseu with the site for incorrect digital certificate, wondering if you can help. in this site: [เข้าสู่ระบบเพื่อดู URL] if you look at its left site of the domain name, you can see it is in red and if you click on its "Not Secure" red text (on Chrome), then click Certifiicate, you will see the warning is about using it is using a different certificate fro...
We have around 500 product labels saved as word documents with the SKU as the file name, each day we get a csv export with a list of SKU's which we require printing. I would like something writing that would automatically print each label in the csv file in the same order it appears in the export. Some may be present more than once.
I need an iPhone/iPad app. I already have a design for it, I just need it to be built.
I have 100 numbers for which I need names. These are all Indonesian numbers. For example, here are 10 sample numbers for you to try out if you prefer and then estimate how much time you need for 100 and then post a bid: Sample numbers: 628112902092 6281281808618 6281398592015 6281363348295 6281649459413 6281295802223 6281373357020 6281298984091 6281363008986
I am looking for a good dotnet developer to help me solve some coding issues. Its a professional work. You should be good with asp.net / c # and MS sql. I need around 40-60 minutes of assistance on a live project. Please get back to me and i will share server and access details. Thanks
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My project is already running on dotNet MVC 3.0 based on Financial System. Now I need to add one more module i.e. Payroll management system integrated with existing system.
I have a long list of company names in an Excel document. I need every website's proper URL by google search. Need a tool or program that can collect company URL automatic. Love to tun the program from my computer. Thanks
I need someone to sift through google to find rock radio stations and compile a list of the station name, names the of radio hosts, and their instagram and facebook names. This will specific to the state of California and for alternative rock stations. I would like this done ASAP. I'm looking for at least 50 radio stations.
I need a baby names website. like bachpandotcom. i have the baby names list,
...and clock company online called watching the clock. We are currently still working on the store before we open. I need someone with a creative mind to think of some unique names for each of my products and design a small description for each. The name just needs to be something that represents the products well where as the description only needs to
All the information is listed on one page, I just need someone to scroll through and copy/paste over the 6k names and addresses into Excel.
I have a list of internet domain names I would like to sell. I need someone to look at this list, find potential / suitable websites that would be interested in buying one of the internet domain names and then contact them (by email) to see if they would be interested in purchasing the domain and then inform me of any replies. *** In your message
I have 10 business names at this moment. Need to write 200-300 words in total for the whole task, 20-30 words per business name. TASK: 1. Research an idea what the business name could mean for a company and where the name could be used. It can be even 2-3 business cases, if the business name have such option. 2. Select 3-4 business area categories
Looking for help coming up with names for a new business. I need: - A Business name ($120 AUD prize) - A name for Product 1 ($50 AUD bonus) - A name for product 2 ($50 AUD bonus) About the company The company designs shelves that transform into different furniture and save space. Like a bookshelf that turns into a dining table, or a desk or
I want a home page with fully load functionality similar to facebook timeline box or twitter functionality. So only experienced candidates are requested to apply.
...com/watch?v=e28z8jHUR-0&list=PLNuit1aMUWTDRll1MGF7Cqn_lX-BqKpZn&index=4&t=0s The desired outcome is to rework the existing project and achieve the following. -Show other player names with 3D Text. -Show chat bubbles when they use the chat. As shown at [เข้าสู่ระบบเพื่อดู URL] Releated information. -Other players are initialized at As
We need to have a large number of images batch renamed automatically based on an intermittent stock number though image recognition. Example being if there are 5 photos and then a photo of a number card "25255" those 5 photos would be renumbered [เข้าสู่ระบบเพื่อดู URL], [เข้าสู่ระบบเพื่อดู URL], [เข้าสู่ระบบเพื่อดู URL], [เข้าสู่ระบบเพื่อดู URL], [เข้าสู่ระบบเพื่อดู URL], then there w...
I need some creative person who is able to create names for my socks. in total i need to have 7 pairs socks named !
I need someone who can create me names for socks ! thy have individual designs and i need someone who is creative enough to name them !
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I need someone to help me with 1. List all stock codes and their company names across, US, Europe, China, Japan and India stock exchanges(all exchanges) 2. List their current executives (CEO, CFO, Managing directors, VPs names)(no contacts), as additional columns against per stock row. 3. Deliver it to me in XLS Format. These open data are readily
Hi Dot Net Desktop Solutions, I noticed your profile and would like to offer you my project. We can discuss any details over chat.
We have a dot net project to develop
I need a php script that I can run that I can run that #1 gets a list of the files in a defined folder (example [เข้าสู่ระบบเพื่อดู URL], [เข้าสู่ระบบเพื่อดู URL], [เข้าสู่ระบบเพื่อดู URL], [เข้าสู่ระบบเพื่อดู URL] and so on) #2 then connects to a mysql db and runs an update query. update tableA set ColumnB = 'value" where ColumnC = 'filename without the e...
Display Invisible full screen google ad (preferably highest payed ad) I have Adsense account already. Let me know what you can do. Message me for more details. Budget: $100 (negotiable)
This is a very simple project. I have a label made with Illustrator and I just need you to make the label in multiple colors with different candel scent names (6 colors with 6 candle scent names). For example, one label will be all green, another all red, another all brown, etc.... I will send you the illustrator file.
There are three domain names I'm planning on selling. Sell [เข้าสู่ระบบเพื่อดู URL] for $200 and get $50 Sell [เข้าสู่ระบบเพื่อดู URL] for $200 and get $50 Sell [เข้าสู่ระบบเพื่อดู URL] for $300 and get $80
Copy paste 532 username and emails in a spread sheet . Start now, finish in 1 hour. Budget : 10 USD Follow the video below . URL and login details will send to you soon. Work flow: I : accept your bid Send you details You: copy paste 5 user details in 5 mins I : check You: continue to complete the 532
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* technical names * buss is into mobile/web app development * with [เข้าสู่ระบบเพื่อดู URL] domain availability
Hello! We're looking for someone who will be qualified on Creative Business name. Expert person needed for this job. I have many more projects like this. If you do great, I'll offer many more ongoing projects. Native English speaker only. More details on chat. No time waster please. Thanks a lot.
I have three different domain names. If you sell any of the domain names to end user, you have a certain percentage from the sale. 1) work4christ dot com is listed on godaddy for $200. sell it and get $50 2) safeinsurance247 dot com is listed on namesilo for $200, sell it and get $50 3) houseandshop dot com is listed on namecheap for $300, sell it and
I need to build about 5 or 6 websites all similar but with different business names example bucks partys hens nights group fitness camps detox centre wellness centre` I need the websites up and running as soon as possible and I need good ad words and seo;s or whatever is needed to make the business close to the top on the first page willing to pay good
We are looking for a developer who will work with our developer on a project. Preferred Location Jaipur only and should not be too far from Raja Park, Jaipur. You will provide 2 seats in your premises one for your provided developer and one for our developer, so he can supervise the tasks him, and they work together.
I need a node js developer for config my vue js app with SCSS - preprocessor and auto short classes name in my dist js/css files like google short name classes
i would like an email list compiled of small retail business in australia, i would like it organised by name, email and state. business should be selling physical produsts
...I am looking for domain and company name for my eCommerce startup. I am planning to sell branded merchandise for celebrity fans. I would prefer catchy and easy to remember names. Need suggestion for following things: 1. Domain name (.com)- should be catchy and easy to remember 2. Company name and company logo(eg apple logo) - to be registered with the
I would like someone to research and prepare a list of bookkeepers who have their own businesses located in the Gold Coast region of Australia. The specific information I would like is: - Business Name - Principal Contact/Business Owner Name - Email Address - Phone Number - Website address (if available) I would like the information in a spreadsheet format. I expect the information would be ava...
...to get what informations are in binary(they need to be info about users and time when users typed in time attendance machine) i ask the factory and they send me a SDK (in dot net or asp.net idk) and thats an example how they proccess binary. so can u take a look at it and fix the php so i can read the binary in proper way u connect via team viewer
I am confused that I don't know what the problem refers to and I get lost
Hello, I have a website [เข้าสู่ระบบเพื่อดู URL] they sell flowers. We need names for the 80 products that are in the website. Simple names nothing too complicated
...job is to edit HTML/CSSJS in order to keep column names on screen when table lines are rolled up on mobile devices. I have an HTML table that uses [เข้าสู่ระบบเพื่อดู URL] in order to keep the column names fixed on top when lines are rolled up. But this doesn´t happen on mobile devices: the column names are rolled up and disappear from the screen when


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Delafloxacin 450mg; tabs.
Melinta Therapeutics
Therapeutic Use:
Indications for BAXDELA:
Susceptible acute bacterial skin and skin structure infections (ABSSSI).
≥18yrs: 450mg every 12hrs for 5–14 days. ESRD (eGFR <15mL/min/1.73m2) or hemodialysis: not recommended (insufficient data). See full labeling.
<18yrs: not recommended.
Boxed Warning:
Serious adverse reactions including tendinitis, tendon rupture, peripheral neuropathy, CNS effects, and exacerbation of myasthenia gravis.
Increased risk of disabling and potentially irreversible adverse reactions (including tendinitis/tendon rupture [esp. in patients >60yrs, or those with kidney, heart or lung transplants], peripheral neuropathy, or CNS effects); discontinue immediately if signs/symptoms occur. History of tendon disorders, peripheral neuropathy, or myasthenia gravis: avoid. CNS disorders (eg, cerebral arteriosclerosis, epilepsy) that increase seizure risk. Discontinue at 1st sign of skin rash, or any other hypersensitivity. Severe renal impairment receiving IV form: monitor serum creatinine levels, eGFR closely; consider switching to oral form if serum creatinine level increases. Discontinue if eGFR falls <15mL/min/1.73m2. Pregnancy. Nursing mothers.
See Also:
Pharmacological Class:
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Adverse Reactions:
Nausea, diarrhea, headache, transaminase elevations, vomiting, other CNS, cardiac, GI, metabolic, musculoskeletal, injection reactions (see full labeling); tendinitis/tendon rupture, peripheral neuropathy, hypersensitivity reactions, C. difficile-associated diarrhea.
Generic Availability:
How Supplied:
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Consignment Service in Lutherville Timonium, MD
YP - The Real Yellow PagesSM - helps you find the right local businesses to meet your specific needs. Search results are sorted by a combination of factors to give you a set of choices in response to your search criteria. These factors are similar to those you might use to determine which business to select from a local Yellow Pages directory, including proximity to where you are searching, expertise in the specific services or products you need, and comprehensive business information to help evaluate a business's suitability for you. "Preferred" listings, or those with featured website buttons, indicate YP advertisers who directly provide information about their businesses to help consumers make more informed buying decisions. YP advertisers receive higher placement in the default ordering of search results and may appear in sponsored listings on the top, side, or bottom of the search results page.
1. Vogue Revisited
I've been a consignor and buyer at this shop for over 12 years and have always had a great and positive experience. The prices are higher than avera…
2. Consign By Design
75 W Timonium RdLutherville Timonium, MD 21093
3. Clearing House Ltd
200 W Padonia Rd Ste 5Lutherville Timonium, MD 21093
4. Time & Again
2011 York RdLutherville Timonium, MD 21093
5. Dandelions Boutique
8401 Saunders RdLutherville Timonium, MD 21093
6. Goodwill Stores
200 W Padonia Rd Ste 4Lutherville Timonium, MD 21093
very difficult to find and not impressed with this location at all! I make handbags and buy skirts for my creations (at a bargain price) and the sel…
7. Better Than New
714 York Rd #170,Towson, MD 21204
From Business: CONSIGNMENT STORE THAT PROVIDE SERVICES LIKE THE EXCLUSIVE BOUTIQUE
8. Sass Upscale Resale Boutique
I can't begin to tell you how much I love this place. I found it while reading the Best Of Baltimore online and had to check it out. The owner, Re…
9. Sass LLC
10866 York RdCockeysville, MD 21030
10. Duckpin Consignment Shop
2350 York Rd.Timonium, MD 21093
11. Purple Peacock
9510 Belair RdNottingham, MD 21236
12. ModiBIZz Online Marketplace
9705 Mill Centre Dr Apt 525Owings Mills, MD 21117
13. Repeat Performance
1426 Reisterstown RdPikesville, MD 21208
3224 E Joppa RdParkville, MD 21234
From Business: Great children's things at great prices all in one place.
15. Hyde & Seek Consignment Boutique
10712 York RdCockeysville, MD 21030
16. Robyn's Nest Clothing Consignment
10872 York RdCockeysville, MD 21030
17. Bugaboo's Closet
17 Hanover RdReisterstown, MD 21136
18. Champagne Chic Consigment Boutique
Baltimore, MD 21282
19. Jones Thrift And Consignment
5305 East DrHalethorpe, MD 21227
20. Angel's Consignment
408 1/2 Bowleys Quarters RdMiddle River, MD 21220
21. Consign By Design
Serving the Lutherville Timonium Area.
22. County Consignment Thift
4106 Annapolis RdHalethorpe, MD 21227
23. Tried But True
I have been shopping at TBT since June and have always found something. I go in about once a month and always come out with cute clothes, a toy or …
24. Play It Again Sports - Baltimore
that has expensive equipment you may want to check this place out. Or if your kid is playing sports and needs expensive equipment this is a great pl…
25. Three Peas Consignments
28 Stocksdale AveReisterstown, MD 21136
26. The Surprise Shop
122 Allegheny AveTowson, MD 21204
27. Local Folks Antiques
3205 Jarrettsvl PikeMonkton, MD 21111
28. Too Good To Be Thru Consignment Boutique
2123 N Charles StBaltimore, MD 21218
29. David's On The Avenue
914 W 36th StBaltimore, MD 21211
30. Same As IT Ever Was Consignment
6300 Eastern AveBaltimore, MD 21224


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X50 – Operation Guide all: And when do we have a working x64 VST version? Digital Recorders D sold out D sold out. This software can be used for the following products as well. However, it is not included in this restriction that you create derivative works based on the data file s. Korg reserves the right to amend any such policies from time to time in its absolute discretion. Wed Apr 15,
|Date Added:
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Sun Mar 29, 8: Korg x50 usb midi any part of this Agreement is found by a court of competent jurisdiction or other competent authority to be invalid, unlawful or unenforceable then such part shall be severed from the remainder of this Agreement which will continue to be valid and enforceable to the fullest extent permitted by law.
Wed Apr 15, Page 77 – midi channel Page 78 – Copying the settings of a combination to Micro X is discontinued korg x50 usb midi it seems that we all are left to our bad Korg devices drivers?
KORG X50 Key Portable Music Synthesizer with USB MIDI
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Software X50 Editor V. I erased every instance of the driver, including the files in C: Vadim Platinum Member Joined: Korg, in no event, will be liable for the direct, derivative, collateral or consequential damage caused by the use of or the inability of using the Licensed Program including but not limited to damage of data, lost commercial profit, interruption of work, lost commercial informationregardless of the degree of damages, and even if Korg x50 usb midi was aware of the possibility of such damages or the possibility of a claim for damage from a third party in advance.
korg x50 usb midi
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The Licensed Program and any manuals or other written documentation supplied with the Licensed Program belongs to you. I guess Sander mixi thinking about a cheab usb midi interface, which you could connect your Korg's midi in and out into. If supplied in conjunction with any Korg instrument or equipment, such Third Party Software shall only relate to the non-essential functions of such instrument or equipment. Korg x50 usb midi you use Grandstage: Then check "Delete the driver software" and click [OK].
Downloads | X50 – KORG USB-MIDI Driver (for Windows 7/) | KORG (USA)
August 1, Note: The complete documentation for the X50 also includes the Operation Guide. If you want to mix the X50's audio signals with the.
Nothing in this agreement shall affect the statutory rights of any person dealing with Korg as a consumer. If you want to apply a plug-in effect to the X50's.
The Voice Name List provides a reference for the X50 data, including: The Licensed Program contains copyrighted information. There is a known problem with Windows in that it only allows seven midi drivers to be installed kkrg any korg x50 usb midi. While not a replacement for the Owner's Manual, this is a friendly "hands on" guide that provides a good place to start in getting korg x50 usb midi with this unit.
Korg X50 Operation Manual: Connecting Midi Devices
You hereby agree to the exclusive jurisdiction and korg x50 usb midi of the aforementioned territory, without regards to conflicts of law provisions. Wed Oct 03, 6: If that's not it, actual usb-midi boxes are very cheap.
Korg x50 usb midi Vista doesn't recognize the new certificate so this new driver doesn't work with Korg x50 usb midi Vista. Korg furthermore disclaims all responsibilities for direct, derivative, collateral or consequential damages caused by the use of or the inability of using the Third Party Software including but not limited korb damage of data, lost commercial profit, interruption of work, lost commercial informationkrg of the degree of damages, and even if Korg was aware of the possibility of such damages in advance.
We recommend that you install it. For more info read the PDF document listed below.
A manual for the software is also included as uab of this download. When the Licensed Program or associated Korg product s is discontinued, the support services will be terminated after 12 months from such discontinuation.


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Oct 19th, 2018
#112: Deb Johnson
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Gus G to guest on new Kamelot albumGus G (OZZY OSBOURNE, FIREWIND) has added his signature style and soloing to one of KAMELOT's new songs that will appear on the band's upcoming CD. Gus G. and Kamelot's Thomas Youngblood comment about the collaboration below:
Youngblood: "Gus and I have been talking about working together for a few years now, I first met Gus when Dream Evil played with us on the Japanese Tour in a few years back. Since then Gus' band Firewind has toured with us in Europe and Japan and we have become great friends. I have always thought Gus' style was very special and very unique, its really great to have him on the new album!"
Gus G. adds: "Kamelot is a very unique band with a sound of their own and I've always liked them. To be invited to play on their new album is an honor and jamming on their track was a lot of fun. During the time our bands toured Japan and all over Europe together, Thomas and I became good friends. I have great memories from being on the road with the guys and I'm glad I contributed to their new album."
Read More News
Tags: Kamelot, Gus G, Firewind, Ozzy Osbourne
Jason Fisher May 18, 2010
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Texas Metal Outlaws Releasing Self - Titled with Heaven and Hell Records and Texas Underground Records - 26 October 2018
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Save on a Wide Selection of Imprint Chaise Lounge with Cushion Best Online Shopping Store. Choose from a wide range of patio-kitchen-furniture..Find great deals Imprint Chaise Lounge with Cushion Great Price Is a quality product.Its ideal for the invaluable as a gift to the people the fond.Or to purchase Incentives for your self.Here we will preferred best products for you.Simply your to learn the products details below.And compare the advantages and disadvantages.
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I stayed here on 2 nights coming and leaving Germany. The staff are very friendly and the hotel is clean. However the first night none of the room fridges worked on the return night only 1 elevator was working. I ate in the hotel dining...More
"I choose the road view over the graveyard view. I was given room no 610 and it was good."
"ask for higher floor rooms. carpets on lower floor levels needs maintenance or replacement."
"Rooms facing the park are more pleasant than the ones facing the huge smoke stack at the front."


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Trailer Rental & Towing in Grand Junction, CO at U-Haul Moving & Storage of Grand Junction
Moving to or from Grand Junction, CO? U-Haul cargo and enclosed trailers are intelligently designed to protect and secure your precious belongings, while our open utility trailers are perfect for hauling building supplies, landscaping materials or bulky items. U-Haul car trailers have EZ load ramps for effortless loading of vehicles while our motorcycle trailers are lightweight and easy to tow. Get FREE trailer rental rate quotes in Grand Junction CO 81504 today.
Get FREE trailer rental quotes on car trailers, utility trailers, cargo trailers, and motorcycle trailer rentals in Grand Junction, CO
Cargo Trailers
Cargo trailers in Grand Junction, CO are lightweight, aerodynamic, and engineered for better fuel economy which makes it easy to tow behind any vehicle. Millions of families use our enclosed cargo trailers to move every year. U-Haul has a large selection of enclosed trailers to rent in-town or one way, review our cargo trailer sizes and reserve a trailer rental online at U-Haul Moving & Storage of Grand Junction!
Utility Trailers
Utility trailers are perfect for towing large or oversize cargo in Grand Junction CO 81504. Many utility trailer rentals come equipped with a loading ramp for easy convenience. The utility trailer selection at U-Haul Moving & Storage of Grand Junction provides an affordable solution for home improvement projects, furniture delivery and for towing recreational vehicles. Utility trailer rentals are also popular with businesses for deliveries and trade-show events. Utility trailers come in a wide range of sizes and styles so that any car can tow.
Car Trailers
Moving cross-country with your vehicle on a car trailer is a smart way to save gas and eliminate miles of wear and tear on your vehicle. Our Tow Dolly is a low-cost option for towing front wheel drive vehicles, while auto transports are ideal for long distance moves. Both car haulers fit most sized vehicles in Grand Junction, CO and are also an economical alternative to calling a tow truck in the case of a break-down. Whether towing behind a U-Haul moving van or your own personal vehicle, you can rent a car hauler for both in-town and one way moves.
Motorcycle Trailers
Motorcycle trailers in are lightweight, easy to tow and have an easy-access loading ramp. Each motorcycle trailer also comes equipped with an integrated chock for the front wheel and with heavy-duty tie-down rings to support even the heaviest of motorcycles. Reserve your motorcycle trailer rental in Grand Junction, CO online today!
Trailer and Towing Supplies
U-Haul trailer hitches
Enter your vehicle information and we'll help you find the right trailer hitch.
Customer Reviews
george t. on
Pulled up dropped and gone. Great
glenn l. on
Truck was ready as promised. Service by agent "Val" was excellent! Check in fast & easy. Truck was easy to drive & use. Truck fee/rate was reasonable & fair. Also had a hand truck, and padding/blankets, but were not used (if used, there would have been an addition charge, which would have been reasonable and fair). Will use again and highly recommend this company.
mitch k. on
I did after hours drop off but it was convenient to the airport and looked to be in good condition.
Larry m. on
Had a problem with one of the tie down straps on the dolly which they took care of as soon as I told them. My trailer plug adapter got left with the dolly when I dropped it off. I went back later and asked if they had found it but they could not, so they gave me a replacement one . Customer service was excellent, will go back when I need to rent again.
DONALD G. on
The rental fee of $19.95 is completely misleading. I drove the gas hog van 28 miles (59 cents per mile) and had it for 2 hours. I had to add 2 gallons of gas ($6) the van consumed to go 28 miles carrying 5 4x8 pieces of rigid insulation. Maybe 10 lbs? On top of that they added some bogus fees. Road safety fee $2 (some made up nonsense). Environmental fee $1. I sure those fees went right in to Budgets pockets. Total for 2 hours $43.22
Judy B. on
Friendly staff and quick service.
Evan G. on
Very impressed with personnel and truck. Powerful truck and trany which made the move that much easier. Thanks
Michael F. on
The staff at this North Ave U-Haul were fantastic...keep up the hard work! Thank you so much!
Joshua L. on
Have more employees available for customers. There were only 2 employees on staff when I returned my rental and it took over 30 minutes for them to check me in
leslie h. on
Excellent job, the attendants were great!!!
alan j. on
Customer line was too long and it took way too long to get waited on. There is a need to streamline this initial process.
ben d. on
Reserved AT equipment was not available at time requested. Had to pick up equipment at another location. Service rep did not seem knowledgeable about the rental process and had to ask another co worker to set up rental for him.
Gregory P. on
After reserving the trailer they sent me to the wrong location.
Debbie H. on
The staff was professional, courteous, and knowledgeable. I couldn't be happier with everything. I will definitely recommend this location. A Plus!
Brian M. on
I was charged more than I was told on the phone it would cost.
Marza M. on
I reserved a trailer for moving. I saw the $50 guaranteed and a confirmation that my trailer would be available. I had family coming in from out of town to help me move. When I arrived to pick up the trailer and was told the one I had reserved was is not available and I would have to drive to Denver to pick one up. That would have been a 7 hour round trip Drive. The trailer I had reserved was sitting there in the parking lot but they said it was reserved for someone else who was going to pick it up in a couple of hours. I got a different trailer and returned it at the end of the day and saw that the trailer I had reserved online was still sitting in the parking lot. I asked about it and was told that person never showed up. I specifically requested the trailer with the rent because I am a 60 year old female and the person coming to help me was recovering from back and knee surgery. I was unable to find anyone local to help me so family came from out of state.
Jeremy M. on
Rude customer service along with long wait times and the van was poorly kept up with
paul b. on
Express check-in failed badly.
bob e. on
We lost the tail lites on this trip.
Bobbie M. on
Very slow to get help.
terrye k. on
Staff was incredible. Very helpful and nice.
Michael D. on
I can not think of anything. Thank you
Rose W. on
A 200 hold on my card and as of Sunday the hold was still there. I would recommend using a company that is going to charge the fee for the uhaul and put money right back on card. I rented the truck Friday. Also would recommend uhaul get better internet service for the WiFi to better help customers
sharon j. on
Francisco I. on
This is my third time renting from this location and we find that everything always is top notch! Highly recommended!