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Monday, 23 September 2013
Summer Projects in the Fall
It is hard to believe how fast Summer flew by!
A lot of us start the Summer out with a big long checklist of the things we want to get done outside while the weather is warm, and some of it does get done, but if it didn't all get done, we hope it's because you took the time to enjoy the great outdoors with your family.
Yesterday marked the first day of Fall. It may be cooling off, but you still have time before the snow comes in.
If a new, or upgraded fence is still on your "To-Do List", consider the following:
This is an ornamental iron fence from the New York Collection at Roma Fence.
It is a classic beauty that only enhances the scenery it is placed in.
Click HERE to view the full New York Collection!
A Chain Link Fence is a great way to go when you don't want to obstruct the view. It comes in a variety of colours and benefits the safety of animals in children.
Click HERE to read more about our Chain Link Fence options, Kennels and Canine Castles.
The traditional Wood Fence is always a great choice.
Our red and white cedar collection can give a very natural look and feel to your outdoor oasis.
For more information about our Wood Fence Options, click HERE!
If none of these fences seem to suit your style, we encourage you to read on.
There are plenty more options:
- Concrete Fence
- PVC Fence
- Snow and Safety Fences
- Agricultural Fence
- Automatic Gates
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Dedicated to exposing "the cabal" those who govern from the shadows, the invisible empire, operating by the numbers, on the daily.
Saturday, July 30, 2016
CNN reporting, 'The democrats Republican moment', DNC Convention analysis
Four flag waving days? Remember, the U.S. flag was sewn at 239 Arch St. in Philadelphia, and it turned 239-years old on June 14, 2016, Donald Trump's 70th birthday. The flag was established June 14, 1777.
Anyhow, this article is a subtle reminder that there is no difference between the Republican and Democratic parties when you get past the rhetoric. Obama and Hillary Clinton are Republicans in every sense of the word, which is why it is so comical that so many Republicans dislike them, and at the same time, so many Democrats support them. It is truly a nation of sheep who just vote for labels, having no awareness of the subject matter or content of what the labels represent.
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subset: commoncrawl


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- What: Counter-Strike, Counter-Strike: Source, Counter-Strike: Condition Zero & Counter-Strike: Global Offensive Pre-Order
- Where: Steam
- Current Price: £12.91
Steam's midweek madness highlight basically asks you to pay slightly (£1) over the odds for a Counter-Strike: Global Offensive pre-order... but you'll also get all the previous game, its Source upgrade and expansion included. Thanks to chaywa at HotUKDeals.
If you're looking for a more retro collection, you might also consider the Counter-Strike 1 anthology. It even includes Ricochet.


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Sept 7, 2012
CHARLOTTE, NC (WBTV) – A Charlotte man is behind bars after investigators say he threatened to kill President Obama during the Democratic National Convention.
Investigators told WBTV that Donte Jarmar Sims, 21, was arrested on Wednesday, charged in a felony criminal complaint accusing him of threatening the president's life.
The threats were done via Sims' Twitter account on Monday morning.
The first tweet was sent out at 10:06 a.m. on Monday when Sims tweeted, "Well Ima Assassinate president Obama this evening !… Gotta get this monkey off my chest while he's in town."


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Best of Transformation Talk Radio: How To Grow Your Business While Everyone Elses Is Shrinking
07/07/2009 07:00 pm PDT
Expert Reveals Tips to Increase Market Share for Business Owners, Managers, and Individuals.
Dr. Pat Baccili
Dr. Pat Baccili helps individuals and organizations break through their limiting Crust so they can reach their unlimited potential. Her specialty is assisting people face t...Find out more »
Grant Cardone
GRANT CARDONE is an International Speaker as well as a NY Times and Wall Street Journal Best Selling Author. His programs have positively affected hundreds of thousands of p...Find out more »


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Index v1.1 – Business Email Template + StampReady Builder
This email HTML template is designed for your business email campaigns. You can promote your business, business application, financial event or seminar, your deals, financial or marketing services, news or whatever you like.
version 1.1
– fixed an issue with StampReady builder module thumbnails
– demo preview link is updated in documentation
Post date: 22/06/2016
Columns: 3
Designers: ThemeForest
Layouts: Responsive
Compatible with: Gmail, Yahoo Mail, Microsoft Outlook, Thunderbird, Hotmail, Apple Mail


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creaseless is a valid word in this word list. For a definition, see the external dictionary links below.
The word "creaseless" uses 10 letters: A C E E E L R S S S.
No direct anagrams for creaseless found in this word list.
Words formed by adding one letter before or after creaseless (in bold), or to aceeelrsss in any order:
n - clearnesses
Try a search for creaseless in these online resources (some words may not be found):
Wiktionary - OneLook Dictionaries - Merriam-Webster - Google Search
Each search will normally open in a new window.
List all words starting with creaseless, words containing creaseless or words ending with creaseless
All words formed from creaseless by changing one letter
Other words with the same letter pairs: cr re ea as se el le es ss
Browse words starting with creaseless by next letter
Previous word in list: creased
Next word in list: creaser
Some random words: pneuma aegis we tipi no hoke bdellium
This is not a dictionary, it's a word game wordfinder.
- Help and FAQ - Examples - Home
Privacy and Cookies Policy - Share - © Copyright 2004-2018 Morewords.com - 18.119mS


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Suitable for Singer models 14U544, 14U554, 14U555, 14SH654, 14SH754, 14SH644,
Product review by Susan Lund from Manchester on Monday, December 12, 2016
Overall rating
Brilliant service, always use original parts as it will extend the life of your machines
Product review by Adele from Oxfordshire on Monday, November 7, 2016
Overall rating
Again, genuine sharp knives make all the difference. Great product and fast delivery
Product review by Edna Watson from Beverley on Tuesday, January 6, 2015
Overall rating
Excellent service, thank you
Product review by Nicola from Kent, UK on Friday, December 30, 2011
Overall rating
Arrived within 24 hours of ordering, just before Christmas! Great service, thank you.


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I kept meaning to update my blog: I really can't even believe that I haven't written anything since APRIL! I'm in such writing withdrawal.
I couldn't remember my password or the font I usually use.
Time to get back into this.
The good news: I am officially finished work in 10 business days.
I will then embark on a super fun wedding/honeymoon vacation with my darling and THEN move to Switzerland!
So this past weekend, my friends threw me a beautiful bridal shower.
Here are some of the fun highlights:
|My hairdresser made my hair look very bridal and fancy
|My friends' made a signature rum punch in my favorite colour
|I enjoyed drinking my mermaid juice throughout the evening
|My bridal party! I have a best man, a maid of honour and bridesmaids...spoiled!
|My talented friend Lando did this portrait of us!!!
|Adorable A+T Bracelet with an aquamarine stone
Today, an article of mine was published on Tiny Buddha.
I am super, SUPER excited about that.
I really want to branch into some sort of motivational coaching/writing- just trying to find my fit!
I know that it'll come.
In the meantime, you can read the article here:
I promise I'll be back this week with more insights and updates.


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Go to the main menu
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About Journal
> Journal Vol & Issue
IEMEK Journal of Embedded Systems and Applications
Journal Basic Information
pISSN :
eISSN :
Journal DOI :
Frequency :
Institute of Embedded Engineering of Korea
Editor in Chief :
Yoo Hyuck
Volume & Issues
Volume 5, Issue 4 - Dec 2010
Volume 5, Issue 3 - Sep 2010
Volume 5, Issue 2 - Jun 2010
Volume 5, Issue 1 - Mar 2010
Selecting the target year
A High Performance NoC Architecture Using Data Compression
Kim, Hong-Sik ; Kim, Hyunjin ; Hong, Won-Gi ; Kang, Sungho ;
IEMEK Journal of Embedded Systems and Applications, volume 5, issue 1, 2010, Pages 1~6
Development of The DCCP for Data Reliability in IP Traffic System
Park, Hyun-Moon ; Seo, Hae-Moon ; Lee, Gil-Yong ; Park, Soo-Hyun ; Kim, Sung Dong ;
IEMEK Journal of Embedded Systems and Applications, volume 5, issue 1, 2010, Pages 7~17
ITS(Intelligent Transport System) as things are used for Broadcast service using TDMB/TPEG/NAVI rather than personal seamless service. It is attaching weight to Traffic information gathering, Charging, Settlement service. This research is applied to improve DCCP(Datagram Congestion Control Protocol) which has function as protecting data and preserving message boundary. The improving method is like that we solve data trust in UDP because Connection and Transmission overhead in UDP is less than in TCP. We fix the data loss which is generated from unordered delivery section of IP base wireless service by using DCCP protocol. We guarantee of connection with OBE(On-Board Equipment) and reliance about transmission of data by complement to mapping table and multi-hoping. Finally, We evaluate the performance about transmission of IP based data. We constructed a test-bed near research center for this test.
Fast Context Switching Architecture in Embedded Systems
Son, Jeongho ;
IEMEK Journal of Embedded Systems and Applications, volume 5, issue 1, 2010, Pages 18~22
In real-time embedded systems, the responsibility is the most important thing because it is related to human life. Context switching is a part of which can slow down the responsibility. We therefore should minimize the amount of state that needs to be saved during context switching. In this paper, we introduce a new architecture (Register Farm) for context switching which can exchange two contexts in one cycle time. Although it might increase the cost of MCU design and the complexity of circuit, it cannot miss any interrupt during context switching. Consequently, Register Farm architecture can make embedded systems spread out in human life because it can increase reliability and responsibility in real time embedded systems.
Design and Implementation of MOST Network Service over POSIX
Lee, Mu-Youl ; Chung, Sung-Moon ; Jin, Hyun-Wook ;
IEMEK Journal of Embedded Systems and Applications, volume 5, issue 1, 2010, Pages 23~28
The automotive industry tries to provide infotainment systems to emerging automobiles. Since the infotainment systems require various peripheral devices and network connectivity, legacy operating systems such as Linux and Windows can be much preferred due to its plentiful device drivers and multimedia applications while the operating systems following OSEK standard are used for automotive electronic control units. Thus it is highly desired that the system software supporting infotainment applications can be portable over different legacy operating systems providing unified programming interfaces. The majority of legacy operating systems support POSIX interfaces for application development. MOST is an automotive network standard for infotainment systems. Network Service defines the protocol stacks for MOST control data, which is essential to implement infotainment applications over MOST. In this paper, we suggest a POSIX-based Network Service so that we can utilize legacy device drivers and applications for automotive infotainment systems. We measure the performance of the POSIX-based Network Service and show that its overhead is not significant.
Testing Environment based on TTCN-3 for Network-based Embedded Software
Chae, Hochang ; Jin, Xiulin ; Cho, Jeonghun ; Lee, Seonghun ;
IEMEK Journal of Embedded Systems and Applications, volume 5, issue 1, 2010, Pages 29~38
It is highly requested that the more complicated embedded software is used for high performance and multiple functions of the systems. This is inevitably increasing the errors. Therefore the embedded software testing is getting important recently. There are not general testing methods which are able to be applied for any embedded systems, but via this research, we introduce a testing method which is based on TTCN-3, a testing standard, for embedded systems. A testing environment for network-based embedded software is implemented with considering the features of TTCN-3 testing which is based on message exchange. The testing environment has two additional parts with TTCN-3 test system, the network analyzer to access the network-based systems and the communication interface which is suggested for embedded systems in previous work, and we have implemented the whole testing environment with interacting these two parts. In addition to the normal testing domain, called single node testing as a unit testing of V-model, we suggest another concept to test multiple nodes in network. It could be achieved by adding keywords such as supervisor and object which are describing the feature of TTCN-3 testing component and generating the TTCN-3 Executable code which contains new keywords. The testing has done for embedded software which is based on CAN network and the demonstration of the testing environment has been shown in this paper.
Energy-Aware Node Selection Scheme for Code Update Protocol
Lee, Seung-Il ; Hong, Won-Kee ;
IEMEK Journal of Embedded Systems and Applications, volume 5, issue 1, 2010, Pages 39~45
As wireless sensor network are being deployed in a wide variety of application areas, the number of sensor nodes in a sensor filed becomes larger and larger. In the past, ISP (In-System Programming) method have been generally used for code update but the large number of sensor nodes requires a new code update method called network reprogramming. There are many challenging issues for network reprogramming since it can make an impact on the network lifetime. In this paper, a new sender selection scheme for network reprogramming protocol is proposed to decrease energy consumption for code update by minimizing overlapped area between sender nodes and reducing data contention. Simulation results show that the proposed scheme can reduce the amount of message traffic and the overall data transmission time.
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The second graders had a great time with this short, focused research project, and they were very proud to share their work on the blog. The last few entries will be posted next week and after holiday break. Please leave comments on these posts if you see something interesting or surprising!
Link to the blog: RES Second Grade Animal Blog


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Vaping Networks are a leading Norway wholesaler of vaping juice and vaping devices with the capabilities of supplying vaping products in Norway.
We supply the best e liquids available at low prices to our retail stores throughout Norway. All our products are of a very high quality and we are not interested in below standard products at any time. Don't settle for inferior products for your vaping store; make sure your vape shop supplier in Norway supplies only high quality products.
Our wholesale vaping company in Norway has a range of high quality products, both for individuals and retailers. Depending on the quantity you purchase, discounts are available.
All products have a detailed delivery and shipping schedule alongside a detailed description of the products.
When you are looking to buy some products for yourself or your shop, then our Norway wholesale vaping shop is a suitable store for you!
Here are some very good reasons why Norway vaping shops choose Vape Networks as their supplier:
All the products we sell in Norway are guaranteed by the manufacturers and we have a straight forward returns policy. Make sure you read all our terms and conditions before using our online wholesale shop in Norway for buying e liquid, electronic cigarettes and vaping accessories.
We aim to always provide clear and easy to understand policies for all our customers in Norway, large and small.


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Risk Sciences Group creates a complete plan for getting the information you need to drive your spending. From initial start to a fully integrated RMIS program, we outline the steps and deadlines.
We communicate with all parties, employers, carriers, TPAs and all necessary internal departments. Status updates keep our clients abreast of progress and tasks. DMITRI is customized per client requests and to fit each department's workflow. Each preference, report, field and dashboard is set for the client. Distributions are set. All users are trained. Multiple rounds of checks test for data accuracy. We work side-by-side with you so you have everything you need to make informed decisions.


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"Our archive represents the most complete record of Satellite AIS vessel movements on a global scale. It is a true testament to our long-standing, and world-leading, operational capability in the Satellite-AIS area." commented Peter Mabson, President of exactEarth. "Furthermore, this unique archive is now growing at an unprecedented rate which reinforces our belief that it will be considered the definitive record of maritime vessel movements on a global scale. With continual improvements being made in our unique ground-based processing capabilities, as well as recent expansions in our superior satellite constellation, we have seen a 50% increase in our operational capability in just the last 6 months. This has resulted in exactEarth now receiving and processing a staggering 1 million AIS messages a day from space for our ever expanding user community. We are excited to announce the availability of exactAIS Archive as it further expands our premier service capability."
exactAIS Archive can be accessed using a wide selection of search criteria, including:
- Date and time range
- Geographical area
- AIS message types
- Ship MMSI list
- Ship type
- Country flag
About exactEarth Ltd.
Based in Cambridge, ON, exactEarth Ltd. is a data services company that leverages advanced microsatellite technology to deliver monitoring solutions characterized by high performance, reliability, security, and simplicity. exactEarth's service, exactAIS™, is a global vessel tracking and monitoring system based on world leading satellite-based advanced AIS detection technology. exactEarth is jointly owned by COM DEV International Ltd (TSX: CDV) and HISDESAT Servicios Estratégicos S.A. For more information, visit our website at www.exactearth.com.
Contact informationNicole Karns
Marketing Communications
exactEarth Ltd.
Tel: +1 519-620-5890
Email Contact


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[caption id="attachment_179425" align="alignleft" width="220"] Getty Images[/caption]
For years people wondered what Butthead meant when he called Beavis a "butt muncher." Then "The Human Centipede" came along to give popular culture a visual. Now, Eric Roberts has joined the list of actors who will be puckering up for next year's "The Human Centipede III (Final Sequence)."
Julia Roberts brother brings quite the resume to the shock horror franchise — he was in"The Dark Knight," "The Expendables" and a lot of TV movies. As Entertainment Weekly pointed out when they broke the "Centipede III" story, he's also got an Oscar nomination, thanks to 1985's "Runaway Train." As if going toe-to-toe with Batman wasn't enough, the supposed "Final Sequence" will put Roberts right in the center of a buzzy pop-culture series that is looking to once again up the ante in gross.
Centipede baddies Dieter Laser and Laurence R. Harvey are both set to return, alongside newcomers Tommy "Tiny" Lister (the dude who will knock you the eff out in "Friday"), Robert LaSardo ("Death Race") and ex-porn star/Charlie Sheen "goddess" Bree Olson. Tom Six will once again write and direct. This one is supposed to feature a (shudder) 500 person human centipede.
Maybe we'll even get to see Roberts do what he does best: Die.


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PEBBLE BEACH >> Rory McIlroy has had a lifetime of storybook mother and father days.
Gerry McIlroy, a skilled amateur, first took his son to the driving range when he was 18 months old. The elder McIlroy also worked 90-hour weeks to support the quickly excelling junior golfer.
Rosie McIlroy took her young son to the driving range when it was snowing because the young golfer knew the proprietors would let him practice. They did.
For several years, the 13-time PGA Tour winner and four-time major winner has reciprocated. His mother travels with him to several events per season. McIlroy's father has played with him in several pro-am divisions in events throughout the world. And he will again beginning Thursday in the AT&T Pebble Beach Pro-Am, which he will play in for the first time.
"It's a tournament that I've always watched and I've always thought it would be really cool to play with my dad," the younger McIlroy said Wednesday. "Fortunately we were able to get him to get him in and play."
McIlroy asked the tournament if the father-son pairing was feasible and officials complied. The younger McIlroy had envisioned the idea for his father's 60th birthday, but the occasion arose a year early than expected.
After finishing second and third last month in events in Abu Dhabi and Dubai on the European Tour, McIlroy, 28, is making his season PGA Tour debut this week. He's playing with renewed enthusiasm after a year of health issues that curtailed last season.
"I'm feeling healthy and I don't think I could have picked a better week to come back over here," said McIlroy, who hasn't played any events in the area. "It's obviously beautiful. It's been a nice few days preparing and seeing a couple of golf courses I've never seen before."
McIlroy's last PGA Tour victory was the Tour Championship in 2016.
"I don't want to say there's been a refocus over the last few years, but just a recalibration of what I need to do to be at that level (one of the world's best players)," McIlroy said. "I think I have a great window of opportunity over the next 10 or 12 years to play great golf and leave my mark on the game."
SPIETH'S FIRST PAYCHECK >> Jordan Spieth first played in the Pro-Am in 2013 via an invitation by tournament director Steve John. A lot has happened in five years. But Spieth hasn't forgotten his first paycheck.
"I had like a 4-footer I missed for a birdie on 18," said Spieth. "In the scorer's tent, they have a sheet where the payout its. I think I got T22 (tied for 22nd) and it was around $65,000 or something.
"I was bummed; all I cared about was that if I made the 4-footer, I thought it was going to have some significance on my status for the next season. And then I sat back over the next week and realized, 'That's cool. My first, my first check was at the event, at Pebble Beach. That was pretty special.'"
CELEBRITY UPDATE >> Huey Lewis, a mainstay of the celebrity field for years, has withdrawn from the tournament due to illness. He was scheduled to play with friend Peter Jacobsen, the 1995 Pro-Am winner.
Adrian Young, the drummer in the rock band No Doubt, will replace Lewis.
Pro surfer Kelly Slater, another tournament veteran, has also withdrawn from the tournament. A replacement wasn't named.


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Search the Community
Showing results for tags 'berwick'.
Found 1 result
Steven Notman Testimonial. Berwick Rangers v Rangers XI.
McEwan's Lager posted a topic in The Bears' DenThought this may be of interest because it hasn't been that well advertised on Rangers sites. A Rangers XI is playing Berwick Rangers in aid of Steven Notman's testimonial year. KO is is 7.45 PM, Wednesday 15 November, Shielfield Park, Berwick. Pay at the gate. 10 pound for adults.


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The boss brought up a good point about review integrity and trust. You do in fact need to earn it. When I started at the site nearly two years ago, I was told to go BUY my games because they wern't sending it to me. Reviewing games for a site is a privelage, not a job.
Alan routinely emails the staff with a list of products that need reviewing and we raise our hands if we want to review it. I've raised my hand a total of two times, I'm more of a collumn writer anyways rather than a reviewer. I COULD review some games, but the problem is just that. I COULD.
I don't need to go into how "The Wrath of El Pato" goes into a streak of four weeks then takes two weeks off. It's a weekly collumn that I have a difficulty of meeting deadling. Half the time it's just becausse the industry is quiet and half the time I'm making fun of current events, but the other half is because I'm employed elsewhere, and I just literally don't have the time to make a quality product.
The point is when people say they are going to review things and do nothing, they not only waste our time as readers, but they also cause a disservice to the site. Someone else could have wanted to review that costly graphics card, and nobody employed by the chief has $500 just lying around to replace it. Our publication is still in its infancy, but we are getting a vast more amount of traffic than half of the sites in our same position. My collumn alone is getting pasted everywhere on other sites. In this infancy, it KILLS us when someone takes one of those expensive products and just pockets it. Because our competiton can review it and we can't. Typically the more expensive, the more well known it is. How crappy would it be if we gave one of those Radeon HD 4800 x2 s to someone and they did nothing? It's a card everyone wants to know about (and it kicks ass by the way). As a reader, if I don't see a review of somthing like that on a site like ours, I say "Bullshit" and move on to the next one.
It's not just the thievery, it's the journalistic integrity and us looking out for ourselves. I'm not a newbie at the site anymore and I still don't think I can get that much expensive stuff even if I asked for it. It's not an insult, it's not them being difficult-they just want to make sure the job gets done and done right.
Believe me, anyone who reviews for us has it good. It's a bitch getting some of those things even if you are the EIC, I should know. Keep in mind to do anything you gotta start at the bottom and eat shit to like the taste of it.


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Reverse Genetics Approaches for the Development of Influenza Vaccines
AbstractInfluenza viruses cause annual seasonal epidemics and occasional pandemics of human respiratory disease. Influenza virus infections represent a serious public health and economic problem, which are most effectively prevented through vaccination. However, influenza viruses undergo continual antigenic variation, which requires either the annual reformulation of seasonal influenza vaccines or the rapid generation of vaccines against potential pandemic virus strains. The segmented nature of influenza virus allows for the reassortment between two or more viruses within a co-infected cell, and this characteristic has also been harnessed in the laboratory to generate reassortant viruses for their use as either inactivated or live-attenuated influenza vaccines. With the implementation of plasmid-based reverse genetics techniques, it is now possible to engineer recombinant influenza viruses entirely from full-length complementary DNA copies of the viral genome by transfection of susceptible cells. These reverse genetics systems have provided investigators with novel and powerful approaches to answer important questions about the biology of influenza viruses, including the function of viral proteins, their interaction with cellular host factors and the mechanisms of influenza virus transmission and pathogenesis. In addition, reverse genetics techniques have allowed the generation of recombinant influenza viruses, providing a powerful technology to develop both inactivated and live-attenuated influenza vaccines. In this review, we will summarize the current knowledge of state-of-the-art, plasmid-based, influenza reverse genetics approaches and their implementation to provide rapid, convenient, safe and more effective influenza inactivated or live-attenuated vaccines. View Full-Text
Graphical abstract
Share & Cite This Article
Nogales, A.; Martínez-Sobrido, L. Reverse Genetics Approaches for the Development of Influenza Vaccines. Int. J. Mol. Sci. 2017, 18, 20.
Nogales A, Martínez-Sobrido L. Reverse Genetics Approaches for the Development of Influenza Vaccines. International Journal of Molecular Sciences. 2017; 18(1):20.Chicago/Turabian Style
Nogales, Aitor; Martínez-Sobrido, Luis. 2017. "Reverse Genetics Approaches for the Development of Influenza Vaccines." Int. J. Mol. Sci. 18, no. 1: 20.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.


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Welcome to OnlineGamingPro.
No Matter Where In the World You Are We Bring you the best deals, highest percentage payouts and progressive jackpots from around the globe. Here at Online Gaming Pro our news and reviews aim to provide you with the most up to date information to help you find your favorite games, and avoid any rogue casinos. Whether you're silly for slots, potty about poker or raving about roulette you can be sure to find it here. We also have your favorite bingo sites, poker rooms and sports book betting sites. So please enjoy your stay and have fun!
Why? There are many reasons for a gambler to choose an online casino. You can pick almost instantly from thousands of casinos and ten thousands of games.
Games have nice interfaces, are user friendly and often have great features that help you with your playing. You could say that you feel like you are right there in a casino. And you can play any game any time.
Also, online casinos give you the opportunity to endlessly play free online casino games in case you are not familiar with them. This way you risk no money before even knowing the rules.
You can use the sign up and deposit bonuses at first, and when you feel confident enough you go and play for money. In a real casino you wouldn't have the chance to practice your favourite game and you wouldn't get any money just for entering their hall. Online casinos are competing against each other by offering their future guests attractive deals


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Asset Realty Management
Welcome to Asset Realty Management. We are here to meet all your property management needs. Whether you are a large company with multiple properties, or have a single family home, we have services and a system to meet your needs.
Serving the greater Knoxville, Tennessee area, Asset Realty Management has a wide range of properties available for rental.
We believe in offering the most comprehensive, cost-effective property management.
As a full service property management company, we handle everything a rental owner would need beginning with advertising your property in multiple venues. We oversee lease signings, security deposit accounting, rent collections, property maintenance, all repair needs, and evictions when necessary. ARM has a state of the art online management program for owners to be able to monitor ALL aspects of their property in REAL TIME. This offers complete financial transparency so that at any time, an owner can view the following:
- tenants payments, balances, ledgers, rent rolls, etc,
- repairs that are presently being conducted and future repair requests by tenants,
- copies of receipts for all bills,
- operating bank accounts and security deposit information,
- balance sheets and profit and loss statements
Here's a short list of the kinds of services we provide:
- Rental Rate Analysis - We perform a survey of the market landscape and determine where the optimal rental rate is that will maximize your revenues.
- Tenant Marketing - We use the latest technology to ensure occupancy is always high and vacancy periods are minimized.
- Tenant Screening - Our thorough screening process protects you from renting to a problem tenant that should have been flagged because of things criminal background or past rental history.
- Rent Collection - Rental income is the lifeblood of your business, we make sure the rent collection process is tight and that delinquencies are are dealt with immediately.
- Property Inspections - We stay on top of the properties we manage and perform regular inspections to make sure the property is being maintained and the lease has not been violated in any way.
- Building Maintenance - We understand that preserving and ideally increasing the value of your properties is one of our chief roles as your management company. We work hard to make sure your properties are clean, healthy and operating efficiently at all times.
- Vendor Negotiations - Our established relationships with reputable local vendors allows us to negotiate the best possible rates for the work you need performed of your properties.
We offer extremely competitive rates and would love to tell you more about us!
- Single Home or Condo (Valued up to $300K)
- Single Home or Condo ($300 to $500K)
- Single Home or Condo ($500K to $1 Million)
- Single Home or Condo (Over $1 Million)
- Multi-Family (2-4 units)
- Multi-Family (5-19 units)
- Multi-Family (20-99 units)
- Office (Up to 9,999 sqft)
- Retail (Up to 9,999 sqft)
- Multi-Family (100+ units)


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Downloads Home » Tests, Tools and Techniques
DocumentsDate added
Order by : name | date | hits [ ascendent ]
- Your Spirituality Quotienthot!
- 18.06.2006
This quick and easy to do test helps you assess your Spirituality Quotient (SQ). The SQ is a useful guide to how engaged you feel with life and what you are doing. The answers can help you fill the gaps that are missing in your life so that you can be happier and more fulfilled.
- WaR IQ - Will Power and Resilience IQhot!
- 07.08.2007
This test is designed to help you understand whether you have the WILL Power and RESILIENCE needed to achieve your goals.
- The Personal Stress Test - A tool which can help you Assess Your Stress Levelshot!
- 05.03.2006
One simple approach for assessing pertsonal stress levels.
- The Organisational Stress Test - a tool for measuring your team or organisational stress levelshot!
- 13.03.2006
- The Impact (Personal) of Stress Testhot!
- 28.06.2006
This is a very quick and easy to use test that helps you assess the impact stress will have on you due to your stress handling abilities/traits and the support you have to help you cope. The test itself is designed to help you "see" where you might need help inorder to cope better and be happier.


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Int. J. Mol. Sci. 2012, 13(9), 11385-11398; doi:10.3390/ijms130911385
Abstract: The FLOWERING LOCUS T (FT) gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. To understand the molecular mechanism of reproduction, three homologous FT genes were isolated and characterized from Cymbidium sinense "Qi Jian Bai Mo", Cymbidium goeringii and Cymbidium ensifolium "Jin Si Ma Wei". The three genes contained 618-bp nucleotides with a 531-bp open reading frame (ORF) of encoding 176 amino acids (AAs). Alignment of the AA sequences revealed that CsFT, CgFT and CeFT contain a conserved domain, which is characteristic of the PEBP-RKIP superfamily, and which share high identity with FT of other plants in GenBank: 94% with OnFT from Oncidium Gower Ramsey, 79% with Hd3a from Oryza sativa, and 74% with FT from Arabidopsis thaliana. qRT-PCR analysis showed a diurnal expression pattern of CsFT, CgFT and CeFT following both long day (LD, 16-h light/8-h dark) and short day (SD, 8-h light/16-h dark) treatment. While the transcripts of both CsFT and CeFT under LD were significantly higher than under SD, those of CgFT were higher under SD. Ectopic expression of CgFT in transgenic Arabidopsis plants resulted in early flowering compared to wild-type plants and significant up-regulation of APETALA1 (AP1) expression. Our data indicates that CgFT is a putative phosphatidylethanolamine-binding protein gene in Cymbidium that may regulate the vegetative to reproductive transition in flowers, similar to its Arabidopsis ortholog.
1. Introduction
Great efforts have been made in understanding Cymbidium biotechnology, particularly tissue culture and transgenics [1–4], and, together with Phalaenopsis, Dendrobium and Cattleya, Cymbidium is one of the most important orchids for the cut-flower and potted plant markets. Chinese Cymbidium, which holds a strong position in traditional flower markets both on mainland China and in Taiwan, is of great horticultural value as an ornamental plant because of its beautiful and fragrant flowers. Chinese Cymbidium includes C. sinense, C. goeringii, C. forrestii, C. faberi, C. ensifolium and C. kanran. C. sinense is a winter blooming epiphytic orchid usually regarded as a Spring Festival flower. C. goeringii is one of the most popular terrestrial species indigenous to temperate eastern Asia, cultivated as an ornamental, and whose flowers are used as ingredients of a soup, an alcoholic drink and tea [5]. C. ensifolium is a popular miniature terrestrial orchid which produces fragrant flowers and is often marketed as a potted specimen [6].
Chinese Cymbidium has a relatively long juvenile phase, taking more than 3 years to flower. Floral development in Chinese Cymbidium is regulated mainly by temperature and photoperiod, although it is still not clear how these pathways affect the genetics of flowering time. Cloning the genes involved in these pathways may elucidate their role during floral bud development. Very little research exists on the role of flowering genes in the regulation of the vegetative to flower transition and flower initiation in orchids, primarily in Oncidium [7,8]. No reports investigating the functions of flowering time genes of Chinese Cymbidium exist. FLOWERING LOCUS T (FT), a well-known floral integrator gene, plays an important role in controlling flowering time [9–13]. In the present study, we report on the isolation and functional analysis of FT gene orthologs from C. sinense "Qi Jian Bai Mo", C. goeringii and C. ensifolium "Jin Si Ma Wei". Ectopic expression of CgFT, which promoted flowering when transformed into Arabidopsis, has been demonstrated. Furthermore, we provide evidence that flowering time in transgenic Arabidopsis plants is altered due to the induction of the flower meristem identity gene AP1, as a result of the introduction and ectopic expression of the CgFT gene.
2. Results
2.1. Isolation of three FT cDNAs from Cymbidium
To investigate the role of the PEBP/RKIP gene family in regulating the transition from vegetative to reproductive growth in Cymbidium, PEBP orthologs were identified and characterized. A combined RT-PCR and RACE strategy was used to clone FT from C. sinense "Qi Jian Bai Mo", C. goeringii and C. ensifolium "Jin Si Ma Wei". CsFT (GenBank accession number HM120862), CgFT (GenBank accession number HM120863) and CeFT (GenBank accession number HM803115) contain 618-bp nucleotides with an open reading frame (ORF) of 531 bp encoding 176 amino acids (AAs), two exons and one intron (161 bp) (Figure 1). The analysis based on AA sequence alignment shows that the three FT AA sequences are identical; they also share a high identity with FT of other plants in GenBank, such as OnFT (94%) from Oncidium Gower Ramsey, Hd3a (79%) from Oryza sativa, and FT (74%) from Arabidopsis thaliana (Figure 2). AA sequence alignment also revealed that CsFT, CgFT and CeFT contain a conserved domain, which is characteristic of the PEBP-RKIP superfamily. The conserved key AA residues Tyr (Y, site 84) and Gln (Q, site 140) in FT homologs were identified in CsFT, CgFT and CeFT protein (Figure 2). The sequence similarity between CsFT, CgFT, CeFT and other FTs indicates that CsFT, CgFT, and CeFT are the putative Cymbidium FT orthologs. The conserved domains were analyzed in FT: LGRQTVYAPGWRQN (14 AAs) and LYN/IYN conserved domain was similar to other FT proteins [14]. The Cymbidium genes characterized here are closely related to the FT gene from monocotyledonous plants (Oncidium and Oryza sativa) [8,15] based on their protein sequences which have a conserved domain LYN (Figure 2). This suggests that CsFT, CgFT, and CeFT are potentially FT orthologs that regulate the transition from vegetative state to flowering and flower initiation in Cymbidium. The AA sequence alignment shown in Figure 2 and the sequences for several other FT orthologs were used to construct a phylogenetic tree for the FT group of genes (Figure 3). CsFT, CgFT and CeFT were grouped within the monocotyledonous FT subgroup and are closely related to Oncidium, followed by Triticum aestivum, Hordeum vulgare and Oryza sativa.
2.2. Different Expression Patterns of Cymbidium FT genes under LD/SD
To explore whether the expression of CsFT, CgFT, and CeFT was influenced by daily oscillations in photoperiod, the expression of CsFT, CgFT, and CeFT mRNA was analyzed every 4 h over a 24-h period in long day (LD) and short day (SD) conditions using quantitative real time PCR (Figure 4). CsFT expression (Figure 4A) was different under SD and LD: it was higher under LD than under SD. Expression of CsFT showed an increasing trend under LD, indicating that expression of CsFT was very sensitive to changes in light under LD. CgFT (Figure 4B) was also regulated by light but its expression was significantly higher under SD than under LD, but there was a similar change in pattern under both conditions, with the level highest at the 4th hour of the light period and lowest at the 16th hour. C. ensifolium "Jin Si Ma Wei" CeFT (Figure 4C) showed higher expression under LD than SD with a rhythmic cycle, but peaked at different times (12 h and 36 h of LD, 20 h and 36 h of SD). CeFT mRNA showed highest levels of expression 12 h into the light period and lowest levels at dawn. CgFT expression was thus different from CsFT and CeFT expression and all were regulated by light.
2.3. Ectopic Expression of CgFT Caused Early Flowering Phenotypes in Arabidopsis Thaliana
Since the three Cymbidium FTs have the same AA sequences, we then randomly chose only one (CgFT) for functional verification. CgFT driven by the CaMV 35S promoter was transformed into wild-type (WT) A. thaliana plants for functional analysis in order to explore whether Cymbidium FT could regulate the transition to flowering in A. thaliana. 35S::CgFT transgenic A. thaliana T0 plants were screened on half-strength MS [16] containing 50 μg/mL Kan using a protocol from Cheng et al. [17] (Figure 5A). In total, 45 independent 35S::CgFT transgenic A. thaliana T1 plants were obtained. All transgenic plants showed identical phenotypes by flowering earlier than WT plants (Figure 5B–D). These 35S::CgFT transgenic plants (Figure 5) flowered at about 15 days after sowing by producing about four rosette leaves (Table 1; Figure 5B). The flowering time of WT A. thaliana plants was more than 30 days and produced nine or ten rosette leaves (Table 1). When transgenic plants were exposed to LD or SD, flowering was obviously promoted under LD (Figure 5E), but was slower under SD (Figure 5F) and ectopic expression of cgFT in A. thaliana was consistent with the AtFT gene expression pattern in A. thaliana [18,19].
To explore whether the early flowering phenotype was correlated with CgFT expression in 35S::CgFT transgenic plants, RT-PCR analysis was performed. As shown in Figure 6, higher CgFT expression was observed in the severe 35S::CgFT transgenic plants more than in the transgenic plants with a less severe or WT phenotype. Further analysis indicated that the promotion of flowering time in severe early flowering 35S::CgFT transgenic plants was also related to significant up-regulation of the flower meristem identity gene AtAP1 in transgenic plants (Figure 6). This result indicates that the function of Cymbidium FT is similar to that of A. thaliana FT in regulating flowering time.
3. Discussion
3.1. Cloning and Characterization of three FT Homologous Genes from Chinese Cymbidium
Flowering time is regulated by a special set of flowering genes [20]. A number of studies indicated that in different plant species, FLOWERING LOCUS T (FT) homologous genes are involved in the earliest stages of flower development [21,22]. Our result shows that the structure of Chinese Cymbidium FT homologous genes contains two exons and one intron. This is not similar to other species, such as A. thaliana [10], Oryza sativa [23], Hordeum vulgare [24], Vitis vinifera [22], Populus sp. [25], and Zea mays [26], all of which have four exons and three introns. However, the wheat TaFT and barley HvFT genes have three exons encoding a 177-AA protein [27], in contrast with four exons and three introns in HvFT2, HvFT3, HvFT4 and HvFT5 of barley [24]. There is a loss of an intron in several barley cultivars, wild barley accessions, and wheat, suggesting that this may be a general feature of temperate grasses [24]. However, we deduced that the lack of introns cannot serve as a common feature of winter plants (i.e., plants that require low temperature for vernalization) because C. ensifolium does not need low temperature for vernalization and has a long flowering period (April-October) in China. C. goeringii, however, needs low temperature for vernalization. For C. sinense "Qi Jian Bai Mo", photoperiod has a major impact on flowering. The origins and importance of spliceosomal introns comprise one of the longest-abiding mysteries of molecular evolution [28]. The rates of loss of existent introns are higher than the rates of gain in eukaryotic evolution, and the rates of loss of introns represent the rate of evolution of a species [29]. It has been suggested that the process of intron birth in early eukaryotes could be fundamentally different from the process in more recent evolution [29]. Interestingly, the Cymbidium FT intron is also different from that of Oncidium, which has four exons and three introns [8]. The loss of an intron in the Cymbidium FT gene may be molecular evidence that Cymbidium is evolutionarily more advanced than Oncidium.
3.2. Expression Patterns and Regulation
FT gene expression increases dramatically when plants are induced by light [15,30]. Our results showed that expression of CsFT and CeFT were regulated by light and were significantly higher under LD than under SD, but that CgFT expression was significantly higher under SD. These results suggest that C. goeringii may be SD-sensitive plants regulated by light, similarly to FT in rice [15], while C. sinense "Qi Jian Bai Mo" and C. ensifolium "Jin Si Ma Wei" may be an LD-sensitive plant like A. thaliana. The difference in expression pattern of Cymbidium FT orthologs in response to light may be due to the fact that the Cymbidium genus contains species with different photoperiodic requirements, unlike A. thaliana, which is an LD plant, and rice, which is an SD plant. This pattern indicates that the expression of CsFT peaked at the 32nd hour of LD and CeFT expression peaked at the 12th hour of LD. However, this is unlike At FT, whose expression level peaks at the 16th hour of the light period and is lowest at dawn under LD conditions [18,19]. Interestingly, although the AA sequence of CsFT, CgFT and CeFT are identical, they have different expression patterns under LD and SD, indicating that expression of CsFT, CgFT and CeFT may also be affected by other factors, such as the upstream gene CONSTANS (CO)-like gene in the photoperiod pathway and other circadian clock genes [31], leading to diverse and complex FT expression patterns. The expression quantity of CsFT and CeFT are enormously higher than that of CgFT under LD, implying that C. sinense and C. ensifolium may primarily be regulated by photoperiod while C. goeringii may be mainly regulated by other factors, such as low temperature.
3.3. Constitutive Expression of CgFT Acts Similarly to Arabidopsis Thaliana FT in Regulating Flower Transition
FT plays a central role as a florigen in floral induction, and its function is conserved across different plant species. The transgenic plants carrying the 35S::CgFT construct flowered earlier (about 23 days from sowing to flowering) and had fewer rosette leaves (average = 4.2) at flowering than the WT plants (average = 10.4). The early-flowering phenotype observed in 35S::CgFT transgenic plants was similar to that observed in A. thaliana [9,10], Solanum lycopersicum [32], Pharbitis nil [33], Vitis vinifera [22], Cucurbita moschata [34], Populus sp. [25], Oryza sativa [35,36], Oncidium Gower Ramsey [7,8] and Glycine max [37] that ectopically express FT orthologs; in all cases, transgenic plants had a flowering time of less than 25 days and the number of rosette leaves at flowering was fewer than five. In contrast, flowering time in A. thaliana and rice plants could be delayed by using RNAi or miRNA of the FT gene [15,35]. Our results suggest that the function of the FT gene in flowering plants is very conservative and that FT and its homologs are required for flowering plants, regardless of photoperiod or species (dicotyledonous or monocotyledonous). Of importance, the level of the AtAP1 transcripts was related to the expression of CgFT (Figure 6): the expression of AtAP1 was higher when FT expression was higher. This indicates that constitutive expression of CgFT acts similarly to AtFT by regulating the transition from vegetative state to flowering by activating AtAP1.
4. Experimental Section
4.1. Plant Materials and Growth Conditions
C. sinense "Qi Jian Bai Mo", C. goeringii and C. ensifolium "Jin Si Ma Wei" plants used in this study were grown and maintained in pots in a greenhouse of the South China Botanical Garden, Guangzhou, China. The pot substrate consisted of Zhijing stone for orchids (Northridge Enterprise Co., Ltd., Taiwan): sieved peat: shattered fir (1:1:1; v/v/v). Arabidopsis thaliana "Columbia" seeds obtained from our lab were surface sterilized in 70% ethanol for 10 s, then immersed in 0.1% (w/v) HgCl2 solution for 10 min followed by five rinses with sterile distilled water. A. thaliana seeds were sterilized and placed on agar plates containing half-strength Murashige and Skoog medium [16] at 4 °C for 2 days. The seedlings were then grown in a growth chamber under LD conditions (16-h light/8-h dark) or SD conditions (8-h light/16-h dark) at 22 °C for 10 days, then transplanted to soil under LD or SD conditions. The light intensity in the growth chambers was 150 μmol m−2 s−1.
4.2. Cloning CsFT, CgFT and CeFT cDNAs from Cymbidium
Total RNA was extracted from young leaves of all three Cymbidium spp. using the Trizol method. The first cDNAs strands were synthesized by reverse transcription PCR (RT-PCR) using M-MLV reverse transcriptase (TaKaRa Bio. Co., Ltd., Dalian, China). The primers (forward: 5′-TAGGACG AGTGATTGGTGA-3′; reverse: 5′-TCACTTGGACTTGGAGCAT-3′) designed for cDNA fragment amplification in PCR experiments were as described for the OnFT sequence from Genebank (ACC59806.1). The amplified fragments contained a partial FT sequence that showed high sequence identity to the FT homologous gene of other species from NCBI Blast. Gene-specific nested primers for 3′-RACE of FT were 5′-TAGGACGAGTGATTGGTGA-3′ (forward), 5′-TTCAGCAGTAGTGG AGCAG-3′ (reverse) designed using the cDNA fragment of FT from Cymbidium. Gene-specific primers (hiTAIL-PCR) [38] for 5′-RACE of FT were 5′-TGGAGCATCTGGATCTACCATGACCTG-3′, 5′-ACGATGGACTCCAGTCCGGCCGAAAGTCCTGAGGTCATTCCCTCCAC-3′, and 5′-CTCGGC TGCTCCACTACTGCTGAAGGC-3′, designed by using the cDNA fragment and the 3′-RACE of FT from Cymbidium. The cDNAs and genomic DNA sequences for the three FT genes were obtained by PCR amplification using forward (5′-CTGAAGGAAGTGATAGCAA-3′) and reverse (5′-AGCAGT GACGCAAAGGAAA-3′) primers designed by using the full-length sequence of FT from Cymbidium after 5′-RACE. The forward primer for FT contained an XbaI recognition site (5′-TCTAGA-3′) and the reverse primer contained a BamHI recognition site (5′-GGATCC-3′) to facilitate the cloning of the cDNAs.
4.3. Real-Time PCR Analysis
Quantitative real-time PCR was carried out using SYBR® Premix Ex Taq™ II (TaKaRa) and ABI 7500 real-time PCR for transcript measurements. Amplification conditions were: 95 °C for 30 s, followed by 40 cycles of amplification (95 °C for 5 s, 60 °C for 34 s, 72 °C for 30 s) and plate reading after each cycle. The primers designed from the full-length sequence of FT from Cymbidium used for quantitative real-time PCR for CsFT, CgFT and CeFT were: forward (5′-AGAGTTGAAG TTGGAGGGAATG-3′) and reverse (5′-GGTCGTTGCTGGGATATCG-3′). The primers for Cymbidium ACTIN (designed using the CsActin sequence, Genebank accession number GU181353) were: forward (5′-AATCCCAAGGCAAACAGA-3′) and reverse (5′-CCATyACCAGAATCCAG-3′). Data were analyzed using ABI 7500 Real-time PCR system Gene Expression software.
4.4. Phylogenetic Analysis
A phylogenetic comparison of aAA sequences of different FT-like homologs was performed using GenBank [39] and aligned with Clustal W2 [40]. Phylogenetic trees based on the complete sequences were generated using MEGA4 [41] and constructed by the neighbor joining (NJ) method. Bootstrap values were derived from 1000 replicate runs.
4.5. Semi-Quantitative RT-PCR
Total RNA was extracted from young leaves using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol. Each RNA sample was treated with RNase-free DNase (Promega, Madison, WI, USA) following the manufacturer's protocol in an effort to remove any residual genomic DNA (gDNA). DNase-treated RNA was subjected to reverse transcriptase reactions using oligo-dT primer and PrimeScript™ Reverse Transcriptase (TaKaRa) according to the manufacturer's protocol. The gene-specific primers (designed by using the full-length sequence of FT from Cymbidium) for FT used in all RT-PCR were 5′-TAGGACGAGTGATTGGTGA-3′ (forward) and 5′-TCACTTGGACTTGGAGCAT-3′ (reverse). The cDNA sequence of the TUBULIN gene in A. thaliana was used as a control and amplified using two primers: 5′-GAGCCTTACAACGCTA CTCTGTCTGTC-3′ (forward) and 5′-ACACCAGACATAGTAGCAGAAATCAA-3′ (reverse) [42]. The primers for A. thaliana AtAP1 used in RT-PCR were 5′-GCACCTGAGTCCGACGTC-3′ (forward) and 5′-GCGGCGAAGCAGCCAAGG-3′ (reverse). PCR was performed with a PCR System LABCYCLER Standard Plus (SENSOQUEST, Hannah, Germany). The following thermocycling conditions were applied: initial denaturation at 94 °C for 1 min; 25 cycles of 94 °C for 30 s, 58 °C for 45 s, and 72 °C for 1 min; final extension at 72 °C for 10 min. The amplified products were separated on a 1.5% agarose gel using 0.1% ethidium bromide (EtBr) and photographed in a Bio Sens SC 710 system.
4.6. Plant Transformation and Transgenic Plant Analysis
The full-length cDNA for CgFT was cloned into the binary vector pBI121 (BD Biosciences, Clontech, San Jose, CA, USA) under the control of the CaMV 35S promoter. The orientation of the construct was determined by PCR and used for further plant transformation. Arabidopsis plants were transformed using a floral dip method [43]. Transformants that survived in medium containing kanamycin (50 μg mL−1) were further verified by RT-PCR analyses.
5. Conclusions
The three Chinese Cymbidium species in this study blossom in different seasons when they are planted in South China. C. sinense blossoms from November to February, C. goeringii from January to March and C. ensifolium from June to October. In this study, we present for the first time, the molecular characterization of FT homologs in their three Chinese Cymbidium species. Amino acid sequence analysis indicated high sequence identity with other FT sequences in other plants. Light period had a profound effect on transcript levels. Ectopic expression of CgFT (the FT ortholog of Cymbidium goeringii) in transgenic Arabidopsis plants resulted in early flowering compared to wild-type plants and significant up-regulation of APETALA1 (AP1) expression. CgFT is a putative phosphatidylethanolamine-binding protein gene in Cymbidium that regulates the vegetative to reproductive transition in flowers. This is important data that sheds light on the molecular mechanisms of flowering in Cymbidium, which is an undocumented phenomenon to date.
This study was supported by the National Natural Science Foundation of China (CNSF31160400; CNSF31101572), Knowledge Innovation Program of the Chinese Academy Sciences (Y021121001) and the Foundation of Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences (201222ZS).
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|No. of plants
|Days to bolting
|Rosette leaf number at bolting
|Days to anthesis
|Rosette leaf number at anthesis
|22.7 ± 3.2
|8.1 ± 1.9
|30.4 ± 5.0
|10.4 ± 2.1
|35S::CgFT (line 7)
|13.0 ± 1.5 **
|4.0 ± 0.0 **
|22.7 ± 3.3 **
|4.2 ± 0.6 **
|35S::CgFT (line 30)
|14.1 ± 2.0 **
|4.0 ± 0.0 **
|23.8 ± 3.9 **
|4.2 ± 0.6 **
|35S::CgFT (line 9)
|16.5 ± 3.7 **
|4.3 ± 0.8 **
|26.6 ± 3.1 *
|4.8 ± 1.0 **
Values are the means ± SD from individual plants.** and *indicate significant differences at p < 0.01 and p < 0.05 according to the t-test compared to wild type.
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).


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I work right by the Museum of Arts and Design, and one day I had my camera with me so I popped into the store during my lunch break just to take a look. I highly appreciate items that are well-designed, pleasing to the eye, and functional (this is one of the reasons why I love the MoMA store!). While browsing, I took some pictures of items that fit the bill.
I also really like wood-based objects. The designs are just so down-to-earth (haha) and organic looking.
One of my favorite finds in this store was actually a book called The Art of Clean Up: Life Made Neat and Tidy by Ursus Wehrli. To understand it you must see it:
For neat freaks like me where I like everything to have a place and in order, this book is a godsend. When I'm older and have my own place – actually, my own coffee table – this book is definitely going on it.
In all, I hope to return to the Museum of Arts and Design and actually take a look at the exhibits. If the store already houses such neat items, I wonder what the installations will be like?
Have you ever been to this museum? What kinds of well-designed inventions have you seen lately?
Thanks for reading!
UPDATE: I did end up going! Read about my experience here.


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A Scam Email with the Subject "Can you receive and manage this fund ?" was received in one of Scamdex's honeypot email accounts on Fri, 05 Dec 2014 23:54:18 -0800 and has been classified as a Generic Scam. The sender was "Mrs. Angela Benjamin" <"mrsanddd@"@sakura.ne.jp>, although it may have been spoofed.
Dear ,
I am Mrs. Angela Benjamin an aging widow suffering Greetings, I am Mrs. Angela Benjamin an aging widow suffering from long time illness. I have some funds I inherited from my late husband, the sum of 15 Million us Dollars and I needed a very honest and sincere Individual or cooperate organization that will use the fund for work of humanity, I found your email address from the Human resources data base and decided to contact you. Please if you would be able to use the funds for the work of humanity as i have stated hear To fulfilled my late husband wishes please , kindly reply me back through my Private email address for more explanations : email@example.com
Mrs. Angela Benjamin


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Opponents of the Obama administration's plan to scrap the "don't ask, don't tell" compromise by which gay men and women are allowed to serve in the military, so long as nobody finds out they are gay, say that with an all-volunteer force stretched thin by two wars, this is no time to rock the boat. They're wrong. Precisely because the military needs all the manpower -- and woman power -- it can get, now is the time to end one of President Bill Clinton's least satisfying triangulations, a discriminatory and unjust policy that never made much sense.
Defense Secretary Robert M. Gates told the Senate Armed Services Committee on Tuesday that he will appoint a high-level commission to study how the Pentagon can transition to allowing gays and lesbians to serve openly in the military. The commission plans to survey top commanders, troops in the field and military families to determine their views on gays in the services and to review how the change would affect Pentagon policy on matters such as whether to provide benefits to domestic partners of gay servicemen, or how to handle the objections of a straight soldier who refuses to bunk with a gay comrade.


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Ellen Drive-In
700 East Mountain View Avenue,
700 East Mountain View Avenue,Ellensburg, WA 98926
500 cars
No one has favorited this theater yet
Located on the south end of town on the Kittitas Highway. It was in operation in 1964.
Contributed by Colin
Recent comments (view all 3 comments)
Mike Rogers on December 20, 2010 at 5:00 pm
It was owned by Mid State Theatres in the late Fifties."ANYONE CAN PLAY" and "SKIDOO" are playing on April 1 1970.On Tuesdays Carloads for 1.75. For awhile in the very early seventies it went by the name ELLEN CINEMA.
Gooper on June 20, 2011 at 5:08 pm
Every year on the 4th of July, the town fireworks took place at the Ellen. Overflow cars lined up on Mtn View St. to watch the show.
jwmovies on September 28, 2012 at 11:51 pm
Now condos. Approx. address for this drive-in was 700 East Mountain View Avenue.
Want to be emailed when a new comment is posted about this theater?
Just login to your account and subscribe to this theater
Just login to your account and subscribe to this theater


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Greetings from the digital frontier!
I just wanted everyone to be aware of a change that is coming down the cables. On Monday, Sept. 24, 2012 we will be implementing a change to the website. For the most part this change will not affect the majority of our customers.
We will be changing the way our URLs are displayed on the website. This change will help with search engines. In this day and age (or nanosecond) search engines make a massive impact on your traffic and we will always need to make sure we do things that help increase traffic.
If you have any articles bookmarked: Please take a few moments and copy the titles of the articles and save them using your favorite text editor. This will give you the ability to find those articles again once we make the switch on Monday.
-Your nerdy web developer Jason


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Geneva College's human services major is designed to provide students with a theoretical and practical framework that will prepare them for positions in a wide array of agencies, including child and youth services, probation, welfare services, government agencies and community centers. These positions require a thorough understanding of public policy regarding administration and implementation of services as well as professional skills within the helping relationship. Geneva's bachelor's in human services provides this training with an emphasis on the consideration of the role of the Christian in service.
Additionally, the human services major draws from the academic disciplines of sociology and psychology as well as focused courses in the domain of social work. Seniors in the major experience an intensive capstone semester of internship in a local service agency. With minimal coursework in biology, human services majors can apply for graduate school in social work.
For Human Services majors, the general career path is to work in social agencies. Careers in human services include, but are not limited to:
Some students choose to attend graduate school primarily seeking an MSW (Master's of Social Work). For those interested in a career in counseling, Geneva College offers a Master of Science in Counseling program. Our graduate students have had outstanding success in the licensure exams, scoring well above the national norms.


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Schedule Your Viewing
DISCLAIMER: The data relating to real estate on this web site comes in part from the Internet Data Exchange program of the MLS of the Wilmington Regional Association of Realtors®, and is updated as of 10/24/2018. All information is deemed reliable but not guaranteed and should be independently verified. All properties are subject to prior sale, change, or withdrawal. Neither listing broker(s) nor The Karen Schwartz Team of North Carolina shall be responsible for any typographical errors, misinformation, or misprints, and shall be held totally harmless from any damages arising from reliance upon these data. © 10/24/2018 MLS of WRAR, Inc.


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Police are investigating a number of reports of burglaries and damage to residents' property in Burgh le Marsh.
Residents around the Station Road and Orby Road areas of Burgh Le Marsh have been victims to shed or garage burglaries and damage to vehicles throughout the early hours of Friday morning.
Wainfleet and Burgh le Marsh Neighbourhood Policing Team are appealing for any information that may assist them with there enquires.
Report any suspicious people or vehicles on 101, referring to incident 103 of 7/10/16.


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By Chris Goldberg
Phillylacrosse.com, Posted 8/22/16
Parkland 2017 defenseman Logan Barlok has committed to play Division III lacrosse at Eastern University.
Logan Barlok profile:
High School: Parkland High School
Graduation Year: 2017
Position: Defense
College Choice: Eastern University
Club Affiliation: Fusion
Major lacrosse honors: The Match All-Star team
What will you major in/study? Athletic Training
Why did you choose this school? "I chose this school because of its amazing coaching and excellent athletic training program."
Outlook for 2017 HS season? "Win the playoffs and go to the state championship."
People to thank: "I would like to thank all my coaches over the years and my family for helping me become the best I can. I would also like to thank Dawn Cipolla for coaching and showing me to lacrosse."
Other schools considered: Messiah and Moravian
Maplezone Sports Institute, the official sponsor for Phillylacrosse.com boys' recruits, congratulates Logan Barlok. MSI is located on Conchester Highway (Rt. 322) in Garnet Valley.


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The Blonde Side: Gentlemen, Prepare for Battle!
The time is here. We have all been counting down the months, weeks, days. . .HOURS until our Dallas Cowboys take the field again as a team, and the wait is over. We can officially drown ourselves in 24/7 gossip and analysis of the Boys' every move (on and off the field) without looking completely obsessed.
The Dallas Cowboys are en route to training camp in San Antonio, but I feel like I am sending my 'Boys off to basic training for the military! I n a sense, they are going to prepare mentally and physically in a way similar to members of the military when joining an Armed Forces Unit. I don't know about y'all, but if we were going to war, I would feel completely confident with the Cowboys as my Army, protecting my rights! The team we have developed is looking stronger than ever and with a little team bonding the next few weeks (maybe they'll take some lessons from their neighbors, the Rangers), we should be unstoppable.
The team is packed with potential and as long as we can maximize each player's potential as a functioning member of the team, I think this could be our year! With all of the AMAZING Twitter/Facebook messages players have been sending out, I know everyone is ready to devote all of their time and energy into making this year a great one for us all. What do you say? Super Bowl 2011 in Cowboys Stadium, Cowboys vs. ______________ (fill in the blank)! I say Cowboys vs. Saints. . .winner takes Kardashian! 😉
What are the 'Boys saying?
Dez Bryant (WR/#88): via Twitter
"Shout out to all of my die hard Cowboys fans"
Tashard Choice (RB/#23): via Twitter
"Welp last night before camp I'll prob try not to sleep and play call of duty all night cause come tomorrow. I'm goin down the green mile."
Michael Hamlin (S/#36): via Twitter
"Headed to training camp. San Antonio here I come"
Miles Austin (WR/#19): via twitter
"One more day until training camp…get ya mind right"
"On the way to San Antonio. Ready to work hard as ever. Shout out to all the Cowboys fans!!! Come to practice and check us out!"
David Buehler (K/#18): via Facebook
"On the bus driving down to San Antonio to start training camp… Gettin my mind right to get this season started. I hope everybody is ready for this to be an epic season for the Cowboys! :-)"
Martellus Bennett (TE/#80): via Twitter
"Cowboy Fans follow me I will keep u posted on camp day to day from a players POV. #RootForTheCowboys or become a 18 wheelers bug shield"
"#RootForTheCowboys or chew glass"
"#RootForTheCowboys or sky dive with no parachute."
"#RootForTheCowboys or bungee jump with fishing string"
"#RootForTheCowboys or kick rocks with open toed sandals"
Tony Romo (QB/#9): via FAKE Facebook
"Training Camp starts tomorrow. I am ready to go. And we got Dez signed up today he is a Cowboy."
Welcome back NFL and fans! It's going to be a tough year so gentlemen. . .prepare for battle!


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Tag Archives: Madhya Nauli
Nauli Kriya – Abdominal Massage in Hatha Yoga Shatkarma
Nauli or Nauli Kriya is a technique for abdominal massage in Hatha Yoga. Benefits of Nauli are tremendous. It is one of the six purification methods or Shatkarma in Hatha Yoga. Nauli practitioners can maintain good health and vitality. But mastering this technique is not easy for beginners. It may take anything from three months to one year to perfect it depending on your body condition, your zeal and your effort.


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A guide to James 'Whitey' Bulger's racketeering trial
James "Whitey" Bulger is on trial in a 32-count racketeering indictment accusing him of a long list of crimes, including participating in 19 killings. Here's a look at the case against him, his defense and what's happening in the courtroom:
The 83-year-old Bulger, the alleged former leader of the Winter Hill Gang, cultivated an early image as a modern-day Robin Hood who gave Thanksgiving dinners to working-class neighbors and kept drug dealers out of his South Boston neighborhood. But that was shattered when authorities started digging up bodies. He fled Boston in 1994 and became one of the nation's most wanted fugitives until he was captured in 2011 in Santa Monica, Calif., where he had been living with his longtime girlfriend in a rent-controlled apartment.
Bulger's former partner and friend, Stephen "The Rifleman" Flemmi, 79, took the witness stand against him Friday. In rapid-fire succession, Flemmi described Bulger's alleged role in a string of killings during the 1970s and '80s. He said Bulger either planned the killings, approved them or committed them himself. Bulger looked straight ahead and took notes on a legal pad. When Flemmi began his testimony on Thursday, the two men exchanged obscenities as court adjourned for the day. Prosecutors have said they expect to rest their case this week.
Bulger's lawyers have not had a chance to cross-examine Flemmi yet. Earlier Thursday, Bulger attorney J.W. Carney Jr. cross-examined a former drug dealer who testified that Bulger demanded $1 million at gunpoint during a 1983 meeting. William David Lindholm described himself as a major marijuana smuggler who distributed along the East Coast. He said he negotiated the amount demanded by Bulger down to $250,000 and agreed to pay it. Lindholm acknowledged that he testified under oath that he would not seek any benefit from his testimony, but later allowed his lawyer to ask prosecutors for a reduction in his sentence for his cooperation in a separate case.
Eleven men and seven women make up the panel of 12 regular jurors and six alternates.
Flemmi is expected to continue his testimony on Monday.


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Hello everyone, I'm at work right now and hope i can get an answer before the end of the day!
Anyway, I'll give you a very short story, a machine was hitting a honeypot of mine. I scanned it with nessus (quite vulnerable) ftp server running...and many other ports...Nessus said that port 445 was open to null sessions. THe nessus plugin is 10394 if you want more info on that. What i want to know is how can i connect with a null session? I was using Net use g: \\host\"" and hten password "" but that doesn't work. Am I even on the right track?
Thank you,


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This hidden treasure in the European centre of Geneva is a good five minutes' walk from the main attractions, but often it's five minutes' walk that can make a real difference. Le Thé – one of many of the city's cosmopolitan offerings – is a Chinese restaurant which also functions equally as a tearoom (hence the name). The main giveaway is the entire wall of teas and teaware that greets you as you enter, all available for purchase at a varying range of prices. However, they also serve a traditional Chinese menu, which is so popular that booking is advisable even on a weekday evening.
Nevertheless, the tea-house theme is prominent throughout, thanks to the leafy bowers, bamboo 'walls' and the use of chunky traditional teapots as decoration (as well as sale items and practical use). You have plenty of time to admire these, as service is on the leisurely side, but the food is worth the wait: the dim sum and steamed breads, available with a variety of fillings, are all excellent, and are washed down easily by the highly palatable (and affordable) Chinese wine on offer. Even if you finish with a gelatinous rice dessert, and amply sample the wide range of teas and other dishes offered, the food is light on the stomach and ultimately a good value offering, with the relaxed pace of service making for a perfect way to unwind. Would definitely return for more of this authentic cuisine and adventurous range of beverages.
Rue des Bains 65, 1205 Geneva, Switzerland
+41 79 436 77 18


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Public Art Opportunity
Council is seeking applications from suitably experienced artists to design and construct a public artwork at Council's administration centre for its children's services programs on Alma Street, Bellerive. Applications close 5.00pm 25 August 2017
The Council is seeking to commission a contemporary Tasmanian artist to create an exciting and engaging contemporary artwork on the blockwork wall in the newly created courtyard space on site at a cost of $10,000+ GST.
Applications close 5.00pm 25 August 2017.


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Comparison of micro-expressions and lie detectors in term of accuracyNowadays, more and more criminal comedies use the idea of body language to help police to solve the case. Moreover, as the new component of body language, micro-expression has been used in some famous American comedy and lie to me is one example. On the other hand, lie has always been an interesting topic through history. In 1977, a University of Southern California social psychologists' study claimed that people lied 200 times per day.(1) Lies can divide into two types: malicious falsehood and white lie (harmless untruth). In spite of the numerous amounts of lies, research estimates that over 75% of all lies are undetected. Researchers keep studying and trying various ways to detect lie in term of accuracy. The most accuracy two has been founded are micro-expression and lie detector. (2)
Micro-expression is a brief involuntary facial expression shown on the face of humans. It is a momentary involuntary facial expression that people unconsciously display when they are hiding an emotion. Haggard and Isaacs first discovered Micro-expressions in the 1960s. In addition, Micro-expression is also one of the most efficient body language that can test whether people lie or not. Not as regular facial expressions, it is difficult to hide micro-expression reactions because it is uncontrollable. Micro means mini, if you have a close-up view of people's expression or action, you can discover that different micro-expression represent different information.
The lie detector has another technical term, which is polygraph, it was invented in 1921 by John Augustus Larson, a medical student at the University of California at Berkeley and a police officer of the Berkeley Police Department in Berkeley, California. (3) Lie detector is the practice of attempting to determine whether someone is lying. It measures and records several physiological directories such as blood pressure, pulse, respiration, and skin conductivity while the subject is asked and answers a series of questions. When a person lies, is assumed that a trained expert can detect whether the person is lying through the physiological changes. The belief is that deceptive answers will produce physiological responses.
Recently, some people try to compare these two methods of testing lies. Some argue that lie detector is much more efficient and reliable to use, as machine is much more objective than human. However, I think that micro-expression is much more accurate in two ways: more specific, more flexible, and indistinguishable.
The role of micro-expression and polygraph
Micro-expressions can express the seven universal emotions: disgust, anger, fear, sadness, happiness, surprise and contempt. Disgust is characterized by the upper lip being raised, generally exposing the teeth, together with a wrinkling of the nose. Anger's micro-expression is composed of the lips being narrowed and pressed together tightly. You will also see the eyebrows slanted down and towards the nose. In fear, you will see the lips horizontal stretched towards the ears. The lower eyelids will be tensed and the upper eyelids will be raised. In sadness, the upper eyelids and outer edges of eyebrows will droop. The subject will appear to have very little focus in their eyes. Also, the corners of the lips will be pulled slightly down. The characteristic of happiness is the smile. In true happiness you will see the corners of the lips turn up and the cheeks will rise slightly. But the key sign of true happiness is the crow's-feet that appear at the corners of the eyes. If you don't see movement from the muscles around the eyes or crows-feet, the smile will be a fake. Surprise can be recognized by widened eyes and raised eyebrows. Also the mouth will open a little bit. In a true expression of surprise, the eyebrows will be raised for less than a second. So if someone is acting surprised, but leaves their eyebrows lifted for more than a second, which means they are lying. It is the only micro-expression that is unilateral. The only one that is biased to one side of the face. (4) All other micro-expressions are equally distributed across the face about a vertical centerline. The Complete betrayal of contempt is the raising of one side of the lips. It can be very subtle and even look like just a twitch.
There are three basic approaches to the lie detector test: The Control Question Test (CQT), The Directed Lie Test (DLT) and The Guilty Knowledge Test (GKT). CQT is often used to determine whether certain criminal suspects should be prosecuted or classified as uninvolved in the crime. DLT tries to detect lying by comparing physiological responses when the subject is told to deliberately lie to responses when they tell the truth. Compared physiological responses with multiple-choice type questions about the crime, GKT choose to use the one contains information only the crime investigators and the criminal would know about. (5)
The disadvantage of Lie Detector
Lie detector can test reality, it can measures and records several physiological directories, such as blood pressure, pluses. Nervousness, anger, sadness, embarrassment, and fear can all be causal factors in altering one's heart rate, blood pressure or respiration rate. (6) However, there are a number of medical conditions such as colds, headaches, constipation, or neurological and muscular problems, which can also cause the physiological changes measured by the polygraph. Thus despite we can find out some emotion change and body symptoms, we can not know the reason which caused this result through the data of lie detector. However, using the micro-expression observation may help us notice the real truth instead of some appearance factors. Moreover, some data showed by lie detector may be useless by estimating the truth of the words. Thus some times we will waste a lot of on doing this kind of research. But micro-expression can easy be used through the interrogation. In other words they do not need to do extra research.
Micro-expression is more flexible
Comparing the micro-expression and lie detector in terms of accuracy, I think micro-expression is more accurate than lie detector because human is more flexible than machine and micro-expression can detect more specific than lie detector. By observing different micro-expression, people can make homologous estimation, as human makes machine. There must be some oversights and limitation. Furthermore, lie detector is confined to explore physiological reaction and it cannot test subtle reaction. (nuance of expression is a kind of inner reveal and hide) (see figure 12)
Figure 13-Micro-expression used in estimating lies
Lie detectors have more limitations than micro-expressions. When we use instrument polygraph, it must inform the person being tested and the participators should be agree with that to load test equipment on the body and to minimize unnecessary movement. It also needs to limited on time and space. However, according to the micro-expression, the person being tested does not need to load a device and restrictions on movement. In other words, the result shows of participators whether lie or not is depends on the detectors choice. For example, people who are testing by lie detector, they may fear that the machine will indicate they are lying when they are telling the truth and that they will be falsely accused of lying. (7) However, by Observing micro-expression, the subject may not know he or she has been tested.
Micro-expression is very useful in wide fields, especially on testing lie. It can be identifying from tinny actions, such as expression in one's eye or simile. Lie detector measures and records several physiological indices. It is also very good for testing lie. Both of them are have many advantages, but micro-expression is more accurate and effective than lie detector because human is more flexible than machine. Although lie detector can test the real things, it cannot test the subtle reaction. Therefore, I think micro-expression is better to use to test lie than lie detector.
Word count: 1296
1 Neal Simon and Mario R. Ventreli, "How I learned to stop worrying about liars and love microexpressions," American Journal of Family Law. 25.4 (Winter 2012): 143, accessed March 17, 2012.
2 Ibid.
3 "Polygraph/Lie Detector FAQs," International League of Polygraph Examiners, accessed March 17, 2012, http://www.theilpe.com/faq_eng.html.
4 "How to Detect Micro Expressions," Ehow, accessed March 17, 2012, http://www.ehow.com/how_5311172_detect-micro-expressions.html.
5 "polygraph ('lie detector'), " The Skeptic's Dictionary, accessed March 17, 2012, http://www.skepdic.com/polygrap.html.
6 Ibid.
7 Ibid.


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- Award Winning Boutique Luxury -
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Offering delicious breakfast and lunch options, an irresistible selection of the finest tarts, macarons and gateaux and of course exceptional coffee made by our talented baristas. Dine in or takeaway - the choice is yours!
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Champagne Club
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Alex and Mandy Braden introduce Encompass:
"Our son Daniel died in a terrorist attack in Bali in 2002. Not because of who he was. But because, to the bombers, he was just one more representative of a set of values that they despised and did not understand".
"It was this realisation that led us to set up the trust. Encompass was founded on the principle that when people truly understand each other, one to one, prejudice and hatred give way to bonds of friendship which transcend political and religious divides.This principle has been proved time and again since the trust was founded in 2003. Encompass is a small charity, funded entirely by voluntary donations. It is supported by a dedicated community of alumni and volunteers, with staff co-ordinators in Indonesia, Israel, Palestine and the UK. In our work and personal stories you will see what we do, and the impact that it has on individuals. However much we may long to do so, we cannot change the past. But we can change the future". Alex and Mandy Braden
What we do
How we do it
UK work
International work
Who we aim to help - We aim to help young people aged 13-25 across five countries including the UK, Palestine, Israel, Indonesia and the USA.