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Three decadent layers of fudgy chocolate cake with a crunchy Oreo cookie buttercream crumb coating. A vanilla creme buttercream for added flavor and chocolate ganache drip, because why the heck not?
Three Layer Chocolate Cake
- 1 3/4 cup all purpose flour
- 2 cup granulated sugar
- 3/4 cup cocoa powder
- 1 1/2 tsp baking powder
- 1 1/2 tsp baking soda
- 2 large eggs
- 1/2 cup vegetable oil
- 1 cup whole milk
- 2 tsp vanilla extract
- 1 1/2 tsp espresso powder
- 1 cup boiling water
Oreo Cookie Buttercream
- 3 sticks unsalted buttercream, room temperature
- 1 pkg (15.55 oz) double stuffed Oreo cookies, 6 to 8 whole Oreos reserved
- 1 cup confectioners sugar
- 1 tsp vanilla bean paste
Vanilla Creme Frosting
- 1 stick unsalted butter
- 1/2 tsp vanilla bean paste
- 3 cup confectioners sugar
- 6 tbsp heavy cream
Chocolate Ganache Drip
- 1 cup + 1 tbsp good quality milk chocolate chips
- 1 cup heavy cream
- 1 tbsp light corn syrup
Three Layer Chocolate Cake
- Grease and flour* (3) 9-inch baking rounds. Preheat the oven to 350°F.
- In a mixing bowl add flour, sugar, cocoa powder, baking powder and baking soda. Mix until combined.
- On low speed add eggs, oil, milk, vanilla and espresso powder. Mix on medium speed for 1 to 2 minutes until the wet ingredients are fully incorporated.
- Carefully add the boiling water and mix on low speed for 1 minute, scraping down the sides and bottom of the bowl.
- Divide the batter evenly between the three pans. Bake for 20 to 22 minutes, rotating the pans halfway if you are using more than one shelf in your oven. Let cool completely before assembling.
Oreo Cookie Buttercream
- Separate creme filling from oreo cookies. Set cookies aside. Don't worry if some of the cookie sticks to the creme.
- Add butter and cookie creme to standing mixer. Beat on medium speed until pale and fluffy.
- Add confectioners sugar and vanilla bean paste. Mix on low speed and increase to medium-high. Beat for 1 minute.
- Using a food processor, pulse the cookies until crumbly or alternatively, place the cookies in a plastic bag and roll with a rolling pin. Mix the cookie crumbs into the frosting on low speed until combined, scraping down the sides if needed.
- Transfer frosting to a large piping bag. Cut bag 1 1/2″ from the bottom so opening is wide or fit bag with a large open tip.
Vanilla Creme Frosting
- In standing mixer, beat unsalted butter on medium-high until pale and fluffy.
- Add confectioners sugar and vanilla bean paste. Mix on low speed and gradually increase speed to medium-high.
- Add heavy cream, one tablespoon at a time, while frosting mixes for 1 to 2 minutes.
- Transfer frosting to a large piping bag. Cut bag 1 1/2″ from the bottom so opening is wide or fit bag with a large open tip.
Chocolate Ganache Drip
- In a medium sauce pan, bring heavy cream to just a simmer. Pour over chocolate chips in a medium bowl. Let sit for 3 to 4 minutes and stir well with a spatula to combine.
- Stir in corn syrup. Let cook 5 minutes then transfer to a small piping bag. Let sit for 15 minutes before using.
Three Layer Chocolate Oreo Cake
- Pipe a quarter size dollop on a cake circle or cake stand to lock in the bottom layer. Pipe a small layer of Oreo buttercream, top with chocolate cake. Repeat one more time.
- Pipe another layer on the top and alone the edges. Using the cake scraper, smooth the frosting on the top and thinly along the sides.
- Refrigerate for 10 minutes to set.
- Pipe vanilla buttercream along the sides, reserving enough to pipe medium stars on top. Smooth out the piping on the sides.
- Refrigerate for 10 minutes to set.
- Cut a small size tip for the chocolate ganache. Starting about 1/2″ from the edge of the cake, slowly pipe a steady stream of the ganache letting it pool and drip over the edge. Working at different speeds and pressure on the piping bag will create different lengths of drip. Cover the top of the cake with ganache and smooth.
- Refrigerate 5 minutes to set ganache.
- Transfer vanilla creme buttercream to a piping bag fit with a large star tip. Pipe medium sized stars along the edge of the cake. Decorate with remaining Oreo cookies.*
*If preparing the cake a day before, wait to garnish with Oreo cookies to avoid them getting soggy during refrigeration overnight.
- Category: Dessert
- Cuisine: Cake


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sin másphrase
1. (general)
No podemos irnos sin más. Tendremos que despedirnos de la anfitriona, ¿no?We can't leave just like that. We'll have to say goodbye to the host, won't we?
Ana ganaba mucho dinero, pero se aburrió del trabajo y lo dejó sin más.Ana was earning a lot of money, but she got fed up with her job and quitted it without further ado.
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text:
Rodeo Schedule
Day Sheets
Other Info
Queen & President Info
Officers & Directors
About AFJRA
Website Designed
Create a Website
List Your Business
"Get your game face on!"
~ Blake Butts
In the same way, let your light shine before others, that they may see your good deeds and glorify your Father in Heaven. Matthew 5:16
Rodeo Schedule
Day Sheets
Other Info
Queen & President Info
Officers & Directors
About AFJRA
2018/2019 Member Application
2018/2019 Rule Book
Updated 2019 AFJRA State Finals Schedule
May 17-19, 2019
Friday, May 17th :
4:30 – 5:30 p.m. Rodeo Office Open for Check In on North Side of Bleachers
Packets will be handed out for all contestants (includes yearbook, banquet tickets, daysheets…)
All Contestants must be in Full Dress Code by 5:30 p.m.
5:30 p.m. – Mandatory Parent and Contestant Meeting in the stands on the North side of arena
** Contestants will be voting on Student Officers for 2019-2020 season**
5:45 p.m. – Line up for Grand Entry
6:00 p.m. – Rodeo Performance #1
Saturday, May 18th :
9:00 a.m. – Cutting (2 Go Rounds)
*** Any ties will be broken 1st -5th Place ***
12:00 p.m. – 2019-2020 Queen Crowning in the Arena
1:45 p.m. – Line up for Grand Entry
2:00 p.m. – 8th Grade Recognition (will be announced in alphabetical order by last name)
2:30 p.m. – Rodeo Performance #2
*** Any ties will be broken 1st -5th Place ***
*** Volleyball Game will start 30 minutes after the rodeo in the arena ***
*** Dance and Goat Roping will begin around 7:30 p.m. on the south side of the arena ***
After the rodeo performance, we would appreciate your help decorating for the banquet
Sunday, May 19th :
9:30 a.m. – Cowboy Church Service
11:30 a.m. – Year End Banquet and Awards
Brother Randy Johnson Award
The Johns family will be sponsoring an award at the AFJRA banquet in honor of Brother Randy Johnson, who passed last week. The recipient will be voted on by members at finals check in. The recipient of this award should exemplify a good attitude, a love for others, and a love for Christ. Brother Randy loved rodeo and our youth. We are also asking members to wear turquoise (Mr. Randy's favorite color) to the Friday evening finals performance.


text:
Posted by susan schmidt on March 13, 2019 at 8:06 am
Viewed 3 times
While traveling in New Zealand in 2018, I came across this wonderful bird with what looks like a cotton ball hanging from its neck. This bird is a tui. One of the best spots to see them was Eden Garden overlooking Auckland.


text:
Tamil Nadu discom to halt wind and solar auctions
Instead, it will buy clean power from the Solar Energy Corporation of India (SECI) to fulfil Renewable Purchase Obligation, a senior state government official told ET.
Orix may buy another 51% in IL&FS Wind Energy
GAIL had offered to pay Rs 4,800 crore for 100 per cent of enterprise value which was at no hai...
Companies have begun to improve the range & accuracy of weather forecasting
Roughly 60% of the operating expenses of a ship are in fuel costs...
Adani Green Energy, ReNew Power only bidders in hybrid auction
Renew Power and Adani Green Energy made techno-commercial bids for 300 MW and 600 MW, respectively, people close to the development said.
Average spot power price to be less than Rs 3.5/unit in May
The peak demand of power this month was 178 GW due to summer season, Mediratta said.
Gujarat wind auction winning tariff remains unchanged at Rs 2.80 per unit
"This bid reflects the positive health of utilities in the state of Gujarat and excellent support from partners," said UB Reddy, managing director of Enerfra.
Power demand soars to a new high on May 8
Hydel, solar and wind power generation touched a total of 831 million units, equivalent to 10 GW of power, and registered a near 40% growth.
Brookfield to buy 2 wind farms from Axis Energy
The addition of Axis Energy's two wind farms totalling 210 MW will take Brookfield's total renewable portfolio in the country to 510 MW.
Wind tariffs don't fall further in latest auction
Tariffs in the wind auction conducted by SECI in February were in the range of Rs 2.82 to Rs 2.84 per unit. SECI is the renewable energy ministry's nodal agency for holding wind and solar auctions.
Copyright © 2019 Bennett, Coleman & Co. Ltd. All rights reserved. For reprint rights: Times Syndication Service


text:
The Championship Player of the Month shortlist was released this morning, and strikers Neal Maupay, Dwight Gayle and Teemu Pukki will battle it out with Blackburn Rovers' Bradley Dack for the award.
Brentford striker Neal Maupay, West Brom's Dwight Gayle, Norwich's Finnish striker Teemu Pukki and Rovers playmaker Bradley Dack are all up for the title of Championship Player of the Month for September.
Brentford striker Neal Maupay has started this season in electric form, scoring five goals in August, and the Frenchman's goalscoring has not slowed down. Maupay has scored four goals in five games, and missed one game through suspension.
West Brom's on loan striker Dwight Gayle has enjoyed a thoroughly successful month at The Hawthorns, picking up five goals and two assists in six games, helping Darren Moore's side go unbeaten in six games through September.
Norwich City's new striker Teemu Pukki has started life at Carrow Road in impressive fashion after joining on a free transfer from Danish side Brondby. The 28-year-old has picked up three goals and one assist as Norwich have risen to 5th place under Daniel Farke.
Last on the list is Blackburn Rovers' attacking midfielder Bradley Dack, as he continues to prove that he is more than capable at Championship level. The 24-year-old only played four matches last month after missing the 4-1 loss against Bristol City through injury. He has netted three times and assisted twice for Rovers.
The winner of the competition will be announced on Friday 5th of October.


text:
Narayana Multi-speciality Hospital has organised a Hernia Evening Clinic at its premises on Feb.1 between 4 pm and 6 pm. For details, contact Mob: 95380-52378, according to a press release.
Uterus cancer screening camp
Narayana Multi-speciality Hospital has organised a uterus cancer screening camp at its premises on Feb.1 and 2 from 10 am to 3 pm. For details, contact Mob: 95380-52378.
Nidhi Apke Nikat on Feb.12
Nidhi Apke Nikat, replacing Bhavishyanidhi Adalat, will be held at the Sub-Regional Office, EPF Organisation, Bhavishyanidhi Bhavan, 2nd Stage, Gayatripuram, on Feb.12 in the following time slots for different stake-holders of EPFO: Subscribers: 10.30 am to 1 pm; Trade Unions and Employers Association: 10.30 am to 1 pm; Employers: 3 pm to 4 pm; Exempted…
Sewing Machine Operator training
Nofa Enterprises, having office at No.836, first floor, opposite Paradise Valley, Naidu Nagar Main Road, Kurimandi, Kesare, has invited applications from eligible candidates for admission to 45-day free training in Basic Tailoring and Sewing Machine Operation. After the training a scholarship of Rs.3,000 will be provided. The candidates must be in the age group of…
National Theatre Festival commemorating Silver Jubilee celebrations, staging of English play 'The women in me' by PKS-Bengaluru, Kalamandira, 7 pm.
Sri Vokkaligeshwaraswamy Temple, Mandi Mohalla
44th annual Linga Pratishtapana Mahotsava, 'Raghavankana Harischandra Kavya' Vaachana by Gamaki K. Ramachandra and Vyaakyaana by Dr. Jyoti Shankar, Vidu. Nagalakshmi Chandrashekar presides, Temple premises, 6.30 pm to 8 pm.
Vocal recital by Vidu. Apekha Appala, accompanied by Vidu. Shruthi on violin, Vid. Deepika Srinivasan on mridanga, Ganabharathi, Kuvempunagar, 6 pm.
Sri Raghavendraswamy Seva Samiti, Jayalakshmipuram
Discourse on 'Mahabharat' by Pt. Sri Vijayindracharya, Raghavendraswamy Mutt premises, Jayalakshmipuram, 5 pm to 6.30 pm.
DoS in Public Administration, Manasagangothri
Late M. Mallikarjunaswamy Endowment Lecture on 'Building Nation – Perspective of Dr. B.R. Ambedkar' by Dr. Siddhalingaiah, former Minister P.M. Narendraswamy inaugurates, in-Charge UoM VC Prof. C. Basavaraju chief guest, Manasagangothri, 11 am.
District Administration
Sri Madiwala Machideva Jayanti, Sri Basava Machideva Swamiji graces the occasion, PWD and District Minister Dr. H.C. Mahadevappa inaugurates, Minister Tanveer Sait chief guest, MLA Vasu presides, Jaganmohan Palace, 11 am.


text:
May 2016
"She was standing on one of the most beautiful streets in Cedar County, maybe even the prettiest in east central Iowa, but the only thing she had eyes for was her book."
~ from Katarina Bivald's
The Readers of Broken Wheel Recommend
Recent Releases
The Past
Tessa Hadley; narrated by Caroline Lennon
Fiction. During a hot English summer, four adult siblings come together in their family's country house for possibly the last time. Their parents (and the grandparents who raised them) are gone, and though the dilapidated estate is full of memories, it may be time to sell it. Like many novels in which family reunions in close quarters bring uncomfortable memories and resentments to the surface,
The Past
is not particularly plot-oriented; instead, it's the characters that captivate -- the adult siblings and the children (and teenagers) they've brought along.
Publishers Weekly
praises narrator Caroline Lennon's "marvelous evocation" of author Tessa Hadley's language.
The Drowning Girls
Paula Treick Deboard
Fiction. Liz McGinnis never imagined herself living in a luxurious gated community like The Palms. Ever since she and her family moved in, she's felt like an outsider amongst the Stepford-like wives and their obnoxiously spoiled children. Still, she's determined to make it work if not for herself, then for her husband, Phil and for her daughter, Danielle. Yet underneath the glossy veneer of The Palms, life is far from idyllic. In a place where reputation is everything, Liz soon discovers that even the friendliest residents can't be trusted and almost everyone has secrets they'd do anything to protect. When the gorgeous girl next door befriends Danielle, Liz can't help but find sophisticated Kelsey's interest in her shy and slightly nerdy daughter a bit suspicious. While Kelsey quickly becomes a fixture in the McGinnis home, Liz's relationships with both Danielle and Phil grow strained. Now even her own family seems to be hiding things, and it's not long before their dream of living the high life quickly spirals out of control.
The Bridge Ladies : A Memoir
Betsy Lerner
Biography. A fifty-year-old Bridge game provides an unexpected way to cross the generational divide between a daughter and her mother. Betsy Lerner takes us on a powerfully personal literary journey, where we learn a little about Bridge and a lot about life. After a lifetime defining herself in contrast to her mother's "don't ask, don't tell" generation, Lerner finds herself back in her childhood home, not five miles from the mother she spent decades avoiding. When Roz needs help after surgery, it falls to Betsy to take care of her. She expected a week of tense civility; what she got instead were the Bridge Ladies. Impressed by their loyalty, she saw something her generation lacked. Facebook was great, but it wouldn't deliver a pot roast.Tentatively at first, Betsy becomes a regular at her mother's Monday Bridge club. Through her friendships with the ladies, she is finally able to face years of misunderstandings and family tragedy, the Bridge table becoming the common ground she and Roz never had.
Once a Crooked Man: A Novel
David McCallum; narrated by David McCallum
Fiction. Actor Harry Murphy has just left an audition in New York when he happens to overhear three guys planning a murder. That's bad enough, but when he flies to England to prevent it, he's mistaken for a mob enforcer and nearly killed himself. With plenty of humor, a plot that threatens to careen out of control, and a large cast, this quirky thriller offers a fun read to those willing to suspend disbelief. This is actor David McCallum's fiction debut; narrating his own audiobook, he effectively distinguishes among the characters through different accents and varied qualities in their voices.
Overload : How to Unplug, Unwind, and Unleash Yourself from the Pressure of Stress
Joyce Meyer
As technology increases your accessibility, it becomes harder to mute the background noise of your life and receive God's guidance. Joyce Meyer calls this
when the demands of your busy life become all-consuming and overwhelming. But to experience the joyful life God has planned, you must make time to focus on His Word. Then you'll receive His healing calmness and gain the strength to take on life's challenges, from physical ailments to problems in relationships. Through the practical advice and Scriptural wisdom in this book, you'll learn how to unplug and free yourself from burdens that weigh you down. You'll gain simple, effective tips for better rest and stress management and discover the fulfilling life you were meant to lead. (Grand Central)
The Travelers
Chris Pavone; narrated by Paul Michael
Fiction. In this complex, intelligent novel, travel writer Will Rhodes is completely unaware that his boss is running a spy agency alongside his travel magazine. In fact, he only learns about it after a strange man invades his hotel room and the woman who had seduced him gives him a one-two punch. From there, a fast-paced international chase ensues, while Will's marriage gets increasingly tense as he tries to discern the truth. If John Le Carré doesn't deliver enough wallop, and Robert Ludlum isn't cerebral enough,
The Travelers
might be right up your alley.
The Bands of Mourning
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Fiction. Legend has it that the fabled Bands of Mourning, ancient artifacts once owned by the long-vanquished Lord Ruler, bestow immense power upon their bearers. Of course, most people in Elendel believe that the Bands are just that: a legend. When evidence of their existence surfaces, Waxillium "Wax" Ladrian must travel to the city of New Seran and investigate, for if the Bands of Mourning are real, they must not fall into the wrong hands. Set in the world of author Brandon Sanderson's Mistborn series (albeit generations later), this fast-paced, dramatic novel follows
The Alloy of Law
Shadows of Self
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The Sound of Music story : How a Beguiling Young Novice, a Handsome Austrian Captain, and Ten Singing Von Trapp Children Inspired the Most Beloved Film of All Time
Tom Santopietro
Musical Films. On March 2, 1965, "The Sound of Music" was released in the United States and the love affair between moviegoers and the classic Rodgers and Hammerstein musical was on. Rarely has a film captured the love and imagination of the movie-going public in the way that "The Sound of Music" did as it blended history, music, Austrian location filming, heartfelt emotion and the yodeling of Julie Andrews into a monster hit. Tom Santopietro has written the ultimate "Sound of Music" fan book with all the inside dope from behind the scenes stories of the filming in Austria and Hollywood to new interviews with Johannes von Trapp and others. Santopietro looks back at the real life story of Maria von Trapp, goes on to chronicle the sensational success of the Broadway musical, and recounts the story of the near cancellation of the film when the "Cleopatra" bankrupted 20th Century Fox. (Ingram)
Everybody's Fool
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Property of a Noblewoman : A Novel
Danielle Steel
Fiction. Faded photographs of a glamorous couple in postwar Europe. Old letters hinting of tragic loss. A breathtaking array of magnificent jewelry, spectacular stones in exquisite settings. These are the contents of a safe-deposit box long abandoned in a New York City bank. If no heir can be identified, the jewelry will be auctioned. But who was the woman who left such a fortune and no will? Jane Willoughby is a law clerk at the surrogate's court and Phillip Lawton a fine arts expert for Christie's auction house. They are simply doing their jobs when they come to the bank to inspect the contents of the box, but for both Jane and Phillip the search turns personal, and their efforts to reconstruct an enigmatic life lead from New York to London and Paris, to Rome and Naples, and a series of stunning revelations. As the truth is slowly revealed, more people are drawn into the puzzle that Jane and Phillip have pieced together, and one among them will inherit the most unexpected gift of all.
Pacific: Silicon Chips and Surfboards, Coral Reefs and Atom Bombs, Brutal...
Simon Winchester; narrated by Simon Winchester
Nonfiction. The Pacific Ocean is the largest body of water on Earth. Turning his keen eye to this behemoth,
New York Times
bestselling author Simon Winchester touches on its geological history, mainly focusing on events after January 1, 1950. Assessing not only the ocean and what's beneath its surface (diverse animals, coral reefs, etc.), he discusses the countries that border it as well as the islands in it. Winchester also addresses humanity's relationship with the ocean and its role in the future as climate change occurs and political power shifts among the countries at its margins. Winchester's own narration of the audio version enhances the personal touch provided in his accounts of traveling around the Pacific region.
Sherryl Woods
Fiction. Despite the wealth and power of his remarkable family, Ben Carlton stayed hidden away in rural Virginia with only his artistic talent and his troubled memories for company. But when he met beautiful gallery owner Kathleen Dugan at the urging of his matchmaking aunt Destiny, his wounded heart began to open. Vivacious, driven Kathleen was intrigued not only by Ben's paintings, but also by the handsome, mysterious man who created them. Were Ben's wounds too deep for Kathleen to mend, or did Destiny create another perfect match?
Check the FVRL catalogue for more great audiobooks!


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The feline immunodeficiency virus (FIV) is a retrovirus of the Lentivirus genus that was initially isolated from a colony of domestic cats in California in 1986 and has now been recognized as a common feline pathogen worldwide. To date, there is only one recent serology-based report on FIV in mainland China which was published in 2016. We designed this study to investigate the molecular prevalence and diversity of feline immunodeficiency virus (FIV) in domestic cats from mainland China. We studied the prevalence of FIV in whole blood samples of 615 domestic cats in five cities (Beijing, Guangzhou, Nanjing, Shanghai and Yangzhou) of mainland China and examined them using FRET-PCR (Fluorescence Resonance Energy Transfer-Polymerase Chain Reaction) and regular PCRs for the gag and env genes. Overall, 1.3% (8/615) of the cats were positive for provirus DNA with nucleotide analysis using PCRs for the gag and env sequences showing the cats were infected with FIV subtype A. This is the first molecular characterization of FIV in mainland China and the first description of subtype A in continental Asia.
Citation: Zhang J, Wang L, Li J, Kelly P, Price S, Wang C (2017) First Molecular Characterization of Feline Immunodeficiency Virus in Domestic Cats from Mainland China. PLoS ONE 12(1): e0169739. https://doi.org/10.1371/journal.pone.0169739
Editor: Pierre Roques, CEA, FRANCE
Received: October 25, 2016; Accepted: December 21, 2016; Published: January 20, 2017
Copyright: © 2017 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper. Data that support the findings of this study have been deposited in GenBank with Gene Accession Numbers (KX646706-KX646707 and KX904833-KX904838).
Funding: This project was supported by grants from the National Natural Science Foundation of China (NO 31272575, 31472225), and the Priority Academic Program Development of Jiangsu Higher Education Institutions and China Scholarship Council.
Competing interests: The authors have declared that no competing interests exist.
The feline immunodeficiency virus (FIV) is a retrovirus of the Lentivirus genus that was initially isolated from colony of domestic cats in California in 1986 and has now been recognized as a common feline pathogen worldwide [1–4]. Infected cats may be asymptomatic for many years during which there is progressive disruption of immune function which might lead to a terminal phase with various clinical infections that is referred to as the feline acquired immunodeficiency syndrome [5]. Transmission of FIV is principally by parenteral inoculation of the virus in blood and saliva, presumably during fighting. Male cats are more commonly infected than females and overall prevalence rates in cats vary geographically, mostly from around 2% to 30% [5].
FIV occurs as seven subtypes or clades (A, B, C, D, E, F and U-NZenv) based on nucleotide sequence diversity of the envelope (env) gene [6–9]. The distribution of the clades varies with subtypes A and B being most common, and occurring very widely [8, 10]. Subtype A is common in Australia, New Zealand, the western part of the United States, South Africa and northwestern Europe [8]. Subtype C has been identified in Europe, Africa, Southeast Asia, New Zealand and Canada while subtypes D and E are found only infrequently, originally in Japan, Canada and Argentina [11–14]. Subtype F has only been described from Portugal and the US and the U-NZenv subtype only from New Zealand [7, 9, 15]. There is only limited data on the genotypes of the latter two subtypes.
To date, there have been five FIV-related reports in Taiwan [16–20], but only little data on FIV in mainland China. A study on wild Pallas' cats from China and other Asian countries identified a unique monophyletic lineage of the FIV most closely related to FIV of African wild cats [21–22].
In the only work on domestic cats, a serosurvey using a commercial test kit (SNAP® Feline Triple® Test, IDEXX Laboratories, Westbrook, ME, USA) found 9% (33/362) of cats studied in Lanzhou, northwestern China, were positive [23]. To provide further information on FIV infections we carried out a molecular survey on cats from five areas in mainland China.
Materials and Methods
The study was reviewed and approved by the Institutional Animal Care and Use Committee of the Yangzhou University College of Veterinary Medicine. Between April 2013 and June 2015, whole blood samples were collected from 615 cats in five cities (Beijing, Guangzhou, Nanjing, Shanghai and Yangzhou) in four provinces of mainland China. The cats from Yangzhou were apparently healthy animals in a shelter while those from the other cities were cats presenting to veterinary clinics for routine health examinations and vaccinations and neutering or with a variety of conditions including fever, stomatitis, and renal failure. All blood samples were collected into EDTA-containing tubes and stored at -80°C until DNA extraction.
DNA was extracted from whole blood samples with the QIAamp® DNA Blood Mini Kit (QIAgen, Valencia, USA) following the protocol of the manufacturer. A negative control, diethylpyrocarbonate (DEPC)-treated ddH2O, was used for extraction after every 24 blood samples to confirm the absence of carry-over contamination during DNA extraction.
The FIV FRET-PCR was performed in a LightCycler 480-II real-time PCR platform as described previously [24]. This PCR method can detect single copies of a 176-bp gag gene fragment of the FIV provirus genome and can be used to differentiate subtypes A to E [24]. Positive controls consisted of nucleotide fragments of the gag regions of FIV subtypes A, B1, B2/E, C and D that were prepared as described previously [24]. Products obtained in the FIV FRET-PCR were further verified by electrophoresis through 2% agarose gels (BIOWEST®, Hong Kong, China), purified with the QIAquick PCR Purification Kit (Qiagen, Germany), and sequenced with forward and reverse primers (BGI Shanghai, China).
The env sequences of eight FIV subtypes (sybtype A: M25381, L00607, X69496, D37813, X69694, M36968; subtype A/B: KP330229; subtype B: D37814, U11820; subtype C: AF474246, AY600517; subtype D: D37811, D37815; subtype E: D84496, D84498; subtype F: DQ072566; subtype U: EF153977, GQ357640) (Fig 1) were obtained from GenBank (www.ncbi.nlm.nih.gov/). The Clustal Multiple Alignment Algorithm was used on the V1-V2 and V3-V4 regions common to the env of all the above FIV subtypes to identify polymorphic regions that would enable us to differentiate between subtypes. The primers to amplify the polymorphic regions were synthesized by GenScript (GenScript, Nanjing, China). Standard PCRs were performed with the primers we designed against a 374-bp segment in the V1-V2 region (forward: GAAGAAGGAAATGCAGGTAAGTTTAGAA; reverse: GGTGCCCAACAATCCCAAAA) and a 680-bp segment of V3-V5 (forward: ATACCAAAATGTGGATGGTGGAA; reverse: TAATCCTGCTACTGGGTTATACCAATT). The primers for the V1-V2 region (first segment of the env) amplify subtypes A to E while those for the V3-V5 region (second segment of env) detect all subtypes (A to F and U-NZenv). Positive controls consisted of FIV subtypes A, B and C identified in a previous study [24]. The standard PCRs were performed in a Roche LightCycler II PCR platform. Each reaction was performed with a 20μl final volume containing 10μl of extracted nucleotides, 1×PCR buffer, 1μM forward primer, 1μM reverse primer, 2 unit Taq DNA polymerase and 200μM dNTP. Thermal cycling consisted of 18 high-stringency step-down cycles followed by 30 relaxed-stringency cycles. The cycling parameters for PCR were 6 × 1 sec at 95°C, 12 sec at 72°C, 30 sec at 72°C; 9 × 1 sec at 95°C, 12 sec at 70°C, 30 sec at 72°C; 3 × 1 sec at 95°C, 12 sec at 68°C, 30 sec at 72°C; 30 × 1 sec at 95°C, 8 sec at 56°C, 30 sec at 67°C, 30 sec at 72°C. Products were verified by gel electrophoresis and sequenced with forward and reverse primers using the Sanger method (BGI, Shanghai, China).
Gag sequences (176-bp) of FIV strains identified in this study and representatives of the five subtypes with sequences in GenBank. In addition, a 374-bp region encompassing V1 to V2 is shown on the left of the bottom panel, and a 502-bp region encompassing V3 to V5 on the right panel. The env sequences of the FIV strains identified in this study (in red) are compared with the sequences of representatives of the FIV subtypes with sequences in GenBank; five for V1 to V2 and seven for V3 to V5. Branch lengths are measured in nucleotide substitutions and numbers show branching percentages in bootstrap replicates. Scale bar represents the percent sequence diversity.
The gag and env sequences we obtained were aligned with similar sequences in GenBank with the Clustalx 1.83 alignment software. Phylogenetic trees were constructed by the neighbor-joining method using the Kimura 2-parameter model with MEGA 6.0. Bootstrap values calculated using 500 replicates.
We analyzed blood samples from 615 cats from Beijing (n = 138), Guangzhou (75), Nanjing (146), Shanghai (143) and Yangzhou (113). Background data was available for 514 cats of which 383 were owned and kept mainly indoors and 131 were strays; 278 were male and 236 were female. Estimated age data was available for 458 cats which were placed into one of the following arbitrary age groups: 68 kittens (< 6 m), 225 young adults (6 m to 4 yrs), 101 adults (4 to 10yrs) and 64 older cats (>10yrs).
The FRET-PCR followed by confirmatory sequencing showed that 1.3% (8/615) of the cats were positive for FIV. All the FIV-positive cats were male cats from Guangzhou (n = 1), Shanghai (3) and Nanjing (4) (Table 1). Seven of these 8 FIV-positive cats were sick with clinical signs such as stomatitis, salivation and anorexia. The melting point and the gag sequence analyses of the FRET-PCR showed all the positive sequences belonged to FIV subtype A. They had 97%-99% (2-5/164 nucleotide mismatches) similarity with the FIV subtype A TN7 strain (GQ422127) from Canada, and 97%-98% (2-4/164 mismatches) similarity with a FIV subtype A CaONA07 strain (AY225009) from Canada.
The sequences of the V1-V2 env region (GenBank accession number: KX710096- KX710097 and KX904827-KX904832) in the positive cats were all similar (90%-97% identity) with six being most closely related to the UK2 strain. This is a FIV subtype A from Scotland (X69494) which has 91% similarity with C18, C172, C176 and C180 (32–34 mismatches) and 93% similarity with C78 and C174 (26 and 28 mismatches, respectively) [25]. In the remaining two positive cats, one (C171) had a strain most closely related to the Sendai1 strain, a FIV subtype A from Japan (D37814) (91% similarity, 32/374), and the other (C181) a strain with 91% similarity to UK2 strain and Sendai1 strain (38/374). (Table 2) [26].
The sequences of the env V3-V5 segment amplicons of the eight positive cats (GenBank accession number: KX646706-KX646707 and KX904833-KX904838) differed by 3%-7% (27–53 mismatches) (Table 3). Five were most closely related to the UK2 strain, a FIV subtype A from Scotland (X69496), with 94–95% similarity (38–43 mismatches) to C171, C172, C174, C176 and C180 (Table 3) [19]. The other three positivities were most closely related to FIV subtype A/B strain FDSydneyC36 from Australia (KP330229) which had 94% (636/677) identity with C18, 96% (652/683) identity with C78 and 95% (638/680) identity with C181, respectively (Table 3, Fig 1) [26].
The phylogenetic trees we generated (Fig 1) that were based on the nucleotide sequences of our mainland China FIV strains and representative strains of FIV from GenBank clearly demonstrated that our Chinese strains were members of subtype A. In addition, the V3-V5 amino acid sequences of the envelop protein for FIV cats in this study were aligned with those of representative strains of FIV from GenBank (Fig 2).
V3-V5 region amino acid sequences of FIV strains identified in this study (red font) were aligned with those of representatives FIV subtypes in GenBank (black for subtype A, blue for subtype A/B; green for subtype B; orange for subtype C, pink for subtype D, and brown for subtype E). Identical nucleotides at given positions are represented by dots (·), gaps are represented by dashes (-).
The results of our study confirm the presence of the FIV in mainland China and add to the known distribution range of the virus in the country. We found a low prevalence but the cats we studied were predominantly indoor pets that had little contact with other cats. Elsewhere, such cats also have a low prevalence of infection, for example 0.7% in the USA [24]. Why we found no infected cats in the shelter population from Yangzhou is unclear, as feral cats often have a high prevalence of FIV infection, for example 18% in the US [24].
Previous studies have shown cats infected with FIV do not have decreased longevity [27] and that it is only after relatively prolonged infection that immunosuppression occurs and clinical signs become apparent [28]. It was unexpected, then, that seven of the cats we found positive for FIV clinically ill although still relatively young (3 years of age or younger). Unfortunately, there was little or no laboratory data available on these cats and we were not able to establish what, if any, role the FIV infections might have played in the clinical signs that were reported.
Previous studies have shown that PCRs for FIV provirus detection can have a wide range of sensitivities (41–93%) [29]. This relatively poor sensitivity might be as a result of the very low levels of provirus that can be present in infected cats, particularly in apparently healthy animals, but can also be due to variability in the proviral genome of the FIV; there can be up to 26% polymorphism between serotypes in the env and gag [30, 31]. Further, recombination with sometimes complex patterns resulting from co-infections or super-infections is also not uncommon in the FIVs [9, 32]. Because of the wide range of subtypes of FIVs and their high evolutionary rate, it is difficult to develop a PCR that is generic enough to amplify all subtypes and yet maintain high sensitivity [33]. The FRET-PCR we used against the gag has been shown to be sensitive, detecting single copies of the target, and capable of differentiating FIV subtypes A, B, C, D and E [24]. Similarly, the primers we developed against the V1-V2 region of the first segment of the env gene amplified subtypes A to E and enabled their differentiation with sequencing. We could not establish if our primers amplified subtype F and subtype U-NZenv as there are no sequence data for this region on GenBank for these two serotypes. There is sequence data, however, for the V3-V5 region of all the FIV subtypes and the primers we developed for this second segment of the env were capable of detecting all subtypes, that is A to F and also U-NZenv. The PCRs we performed in our study thus enabled us to detect low copy numbers of FIV and also to detect all the recognized subtypes.
In our study, all the FIV positive isolates we detected belonged to subtype A which occurs widely around the world with most isolates being from Australia, New Zealand, North America, South Africa and Europe [10, 34]. Isolates from countries closer to mainland China have included subtypes A, B, C and D from Japan [14], subtype C from Korea and Vietnam [13, 35], and subtype D from Thailand [36]. Our description of subtype A in mainland China is thus the first description of this subtype in the country and, to the best of our knowledge, on the mainland of Asia.
Of note is our finding that the sequences of the second segment of the env in three of our mainland China FIV strains (C18, C78 and C181) were very similar to the FDSydneyC36 (41, 31 and 42 mismatches, respectively) (Table 3, Fig 2). The sequences of the first segment of the env, however, were relatively distant (91, 91 and 98 mismatches, respectively), being more closely aligned with representatives of the FIV subtype B (Table 2, Fig 2). This difference is explained by the fact that the FDSydneyC36 strain, from a cat immunized with a commercial FIV vaccine [26], is a recombinant strain of FIV, subtype A/B. The second segment of the env is assigned to subtype A while the first segment is assigned to subtype B.
Our findings of FIV subtype A and the serological evidence of infections presented by Cong et al. [23] should alert Chinese veterinarians to the possibility of infections in their feline patients. Although clinical signs resulting from FIV infection are highly variable and unpredictable, cats infected with subtype A have been found to remain asymptomatic for longer and have lower viral loads than cats infected with subtype C [34, 37, 38]. The subtype A FIV strains are often neurotrophic and can produce neurological signs, most commonly behavioral changes but also seizures, paresis, multifocal motor abnormalities, impaired learning and disrupted sleep patterns [5]. Currently there is only one registered FIV vaccine which is composed of two FIV subtypes, A and D. The vaccine is reported to confer protection against subtypes A, B and D and might then be useful in mainland China where [32, 39], to the best of our knowledge, vaccination is seldom if ever performed. A recent study, however, has shown the vaccine does not confer solid protection and breakthroughs were found with FIV subtypes A, F, A/F and D/F [40]. Further studies on the usefulness of vaccination under conditions of natural challenge are required, particularly in Asian countries where subtype C is prevalent.
In conclusion, our study has shown that FIV subtype A occurs in mainland China and continental Asia. Larger studies are indicated to further determine the subtypes present in the region which will facilitate the development of accurate diagnostic tools and control programs.
This project was supported by grant from the National Natural Science Foundation of China (NO 31272575, 31472225), and the Priority Academic Program Development of Jiangsu Higher Education Institutions and China Scholarship Council.
Author Contributions
- Conceptualization: CW JZ SP.
- Data curation: JL LW JZ.
- Formal analysis: JL LW JZ.
- Funding acquisition: CW.
- Investigation: JL LW JZ.
- Methodology: CW PK.
- Project administration: CW.
- Resources: JZ CW.
- Software: JZ SP CW.
- Supervision: CW PK.
- Validation: PK SP.
- Visualization: CW JL JZ.
- Writing – original draft: CW PK JZ.
- Writing – review & editing: CW PK JZ JL SP.
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text:
Many are surprised to learn that kids are at risk for packing on an unhealthy number of pounds during the summer break.
In fact, data from national surveys indicate children's body mass index can increase nearly twice as fast during the summer months as compared to the school year.
Findings from these reports also show children of certain demographics -- mainly Hispanic and African-American-- are most prone to putting on weight when school is out.
Marie Roth, registered dietitian for Blythedale Children's Hospital and Kohl's Eat Well, Be Well program offers parents some healthy summer tips:
Keep meal and snack times consistent
- Schedule meals around the same time each day and offer healthy snacks in between to tide kids over to the next meal. When school routines and mealtime schedules go out the window, kids tend to "graze" or nibble on foods all day — often on processed "junk" foods — taking in significantly more calories than they normally would. Constant nibbling can cause kids to lose touch with their body's internal hunger and satiety cues. As much as possible, have set meal and snack times and encourage water only in-between.
- Be sure to stock up on healthy foods. Kids can't eat or drink empty calories that aren't there. Naturally low in calories, fruits and vegetables contain lots of water and fiber which can make us feel fuller on much fewer calories. Aim for a minimum of 5 servings each day or fill half your plate at meals with fruits and vegetables. Meals should be made from wholesome foods such as whole grains, beans, lean proteins, fruits, vegetables and healthy fats such as olive, avocados, nuts, seeds and olive oil.
- Fast foods should be eaten no more than once monthly. Sweets or treats should be eaten can be eaten occasionally, but in moderation. When eating things like ice cream, cookies and chips, it's a good idea to not eat from the bag or container, which can lead to eating larger portions and extra calories. Instead, serve a small portion on a small plate or cup. Always eat meals and snacks at the table and away from the TV or computer to promote more mindful eating.
Think before you drink
- Hydration is important in the summer heat. Plain water is the best choice. Kids should be drinking mostly water throughout the day and milk at mealtimes. Kids do not need juice, and instead should eat whole pieces of fruit. When serving juice, be sure to serve only 100% juice.
Also follow these recommended limits:
- up to 6 months old: no juice at all
- 6-12 months old: no more than 2-4 ounces per day, always served in a cup
- 1-6 years old: no more than 4-6 ounces of juice per day
- 7-18 years old: no more than 8 ounces of juice per day
- Avoid sugar sweetened drinks. Recent studies suggest the more sugary beverages a person has each day, the more calories he or she takes in later in the day. This is the opposite of what happens with solid food. When we eat high calorie meals, we tend to take in fewer calories at a later meal. This compensatory effect doesn't seem to be present after consuming soft drinks.
- Choose sports drinks only with intense and continuous exercise greater than 60-90 minutes accompanied with heavy sweating. Sports drinks combine water with vitamins, minerals, and electrolytes — substances that help regulate body processes that are lost with excessive sweating. But these drinks are unnecessary for ordinary, everyday activities and normal bouts of exercise. They also contain added sugars or artificial sweeteners and offer little nutrition for the calories. Sugary beverages slow down fluid absorption by the body and make us feel thirsty again soon after.
Maintain proper sleep schedules
- It's fine to let the kids stay up a little late occasionally during the summer break, but implementing a bedtime and healthy sleep routine is important. Recent studies suggest late nights and inadequate sleep, even just 1 hour, play a role in weight gain by increasing the production of cortisol, a stress hormone that triggers weight gain in the abdominal area, while decreasing the production of leptin, a hormone know for regulating the appetite--which can lead to increased hunger and snacking. It's best to set bedtimes and wake-up times close to what they are during the school year. Young, school-aged children need 10-12 hours of sleep, and adolescents and teens require 8-10 hours each night.
Avoid the lazy days of summer
- Limit long bouts of leisure activities that are sedentary and look for more fun ways to be active. Take screen breaks and go for a quick walk, bike ride or play a sport for 20-30 minutes every few hours. Kids need a minimum of 60 minutes of exercise daily. Breaking up the sedentary activity is sometimes more feasible than trying to do build in several hours at once. Keep in mind the recommended screen time limit is two hours per day. If it's too hot outside, consider getting out early or at dusk when the temperatures are more tolerable or find a cool water activity to do like water balloons, sprinklers, the local swimming pool or a day trip to the beach.
- Give them chores. Yard or household tasks like sweeping, vacuuming, laundry, dishes, changing sheets, meal prep or washing the car offer kids a great way not only to learn life skills, contribute to the family, and burn energy while they're at it.
Plan ahead for travel, day trips and summer activities
- Eating healthy on the road and while vacationing can be a challenge. While traveling, instead of hitting the snack bar or spending the week grabbing fast foods, funnel cakes, ice creams, chips and sodas--try packing a small cooler full of healthy options like sandwiches on whole-grain bread, cut up vegetables, cold pieces of fruit, and water for the car ride, beach days or swim club.
- Heading out for a picnic or BBQ? Make sure to include lots of fruits and veggies which contain lots of water and fiber that help us feel full on fewer calories. Watermelon, fresh berries, corn on the cob, a fresh tomato salad, or grilled veggie kebabs are typically a hit with all ages. Remember, treats can be part of summer's fun, but they should be eaten in moderation.
Kohl's Eat Well, Be Well Program
Blythedale Children's Hospital, through the generosity of Kohl's Department Stores, is proud to offer Blythedale and Kohl's Eat Well, Be Well, an innovative outreach program designed to bring health and nutrition education to schools throughout Westchester and Putnam counties. Through this program, Blythedale staff members teach healthy eating habits to children by providing curricula, training and educational tools to school districts throughout the area. The program provides general nutrition guidelines to students, parents and school faculty. Blythedale Children's Hospital offers experts in nutrition and health-education to speak with local parenting groups, PTAs and school personnel.
©2014 Blythedale Children's Hospital. All contents of this site are the express property of Blythedale Children's Hospital and may not be reproduced without our express written permission.


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|Brand Name
|Read Customer Reviews
Add style storage space with the Mainstays 6-Cube Organizer. It offers versatile storage options. It provides a convenient way to hold craft & hobby materials, toys, media, office supplies & more. This six-cube storage organizer features a stacking design that is ideal for quick organization, eliminating clutter, and maximizing space. You can customize the configuration and change up its look with collapsible bins or baskets (sold separately). The 6 storage cube organizer includes three open-backed & three backed cubes. It can be used to display & store a variety of items. This item is also available in different colors.
Mainstays 6-Cube Organizer, Multiple Colors:
- Number of cubes: 6 compartment storage cube
- Assembled dimensions: 23.75"W x 11.75"D x 35.38"H
- Cube opening: 11.13"W x 11.13"H
- Available in Black Stipple, gray and white
- Mainstays cube storage has assembly required
- Package dimensions: 4.17"H X 39.53"L X15.28"W
- 3 open-backed cubes and 3 backed cubes
- Provides display and storage space
- Compatible with collapsible bins and baskets which are sold separately
- Customize the configuration to create different looks
Customer Reviews
Great price, bedside table alternative
Perfect as a night stand next to our tall bed. Like that i can use it as both shelves and spots for baskets. Good amount of space on top gor all of my things. Only thing i dont like is the back, wish it was a full back.LEandCE
Bought this in store, item was heavily damaged when I took it out of the box. unfortunately I needed to use it right away so I was unable to return it. I put it together and "dealt" with it.JW
I ordered the shelf and was told it would be ready to be picked up the next day. Well, they sold it and had to order one, it took 3 days to get it to the store. I finally get to pick up my shelf, get home and unbox it, the corners are damaged, the sides are scratched, there are NO INSTRUCTIONS, so I tried to look up instructions on Google and theres not even enough pieces to build this shelf!
DONT WASTE YOUR MONEYJujukazoo
The box was fine but as soon as I opened it you could see the damaged sides (paint chipping off, corners busted up) Also had hardware missing, luckily we had extras.steph
Easy to build and decent price.Handy


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Right Here, Right Now: On Albert Maysles' In Transit
Aboard Amtrak's Empire Builder, a passenger train that travels between Chicago and the Pacific Northwest, we capture sight of an elderly man with a digital camera in tow. He reflects on the images he captured on his DSLR, including one of a young passenger: a pregnant woman who's four days overdue and taking the train to meet with friends. The photo is a candid one as she leaves the train and makes her way down an icy path that leads to the terminal. Solemnly, the man comments that to cameras that he's ill and doesn't have much time, that he doesn't want to die without having a good look at the world. It's to Albert Maysles' credit that what we have in In Transit (Recommended) is a clear-eyed, optimistic, and sincere look at American life.
What happens to the man, the pregnant woman, or any of the commuters we encounter aboard the Empire Builder is a mystery. What we do know is that Maysles passed away shortly before In Transit was first screened in 2015. The director, a totem of documentary filmmaking alongside his brother David, started in the 50s and long spoke of composing a documentary entirely based on his interactions with people on the train. Garnering financing for such an effort is, as you imagine, unimaginable. But after a lengthy legal battle that prevented the film from screening for nearly two years, the film finally makes its way to Chicago's Gene Siskel Film Center for an exclusive run.
It's a lovely coincidence that Maysles' film screens just a week before Frederick Wiseman's new film, Ex Libris: The New York Public Library, premieres at the Siskel, as the two filmmakers complement one another in distinct ways. While Wiseman is content in observing the social order uninterrupted, so often capturing subjects at their most fraught and fragile, Maysles offers a conciliatory ear for which he allows his subjects to confess their concerns. As Wiseman yearns to be an unseen microscopic object, Maysles makes no effort to conceal the eye of his camera, instead offering it as a platform for his subject to grapple with their own existential plights. And for a film that speaks to the probing questions of where we're going and how we're getting there, this platform becomes vital in imbuing the film with a tactile warmth and humanity. The brief passages we have with various passengers of the Empire Builder offers moments that are equal parts beguiling as they are heart-wrenching. From a mother discussing how her daughter, who she had not seen for decades, urged her to take on a more youthful look, to a young man finding himself in love for the first time, Maysles suggests that what may be most "natural" is to speak from the heart. It's a different approach from Wiseman's more cerebral approach, but no less effective in capturing what it means to be a human.


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In 2019 we find ourselves at a crossroads, with a greater proportion of invested funds being deposited with ethical funds than ever before. It's a trend that's been on the rise since the 1970s, but in the present day, we're seeing a major shift amongst consumers and savers of all types, towards a more socially and environmentally conscious approach to the business of making money.
With so many Americans being keen to be viewed as socially responsible, being green is a great way to drum up private investment for companies and projects. It's been estimated that more than 8 trillion US Dollars are currently invested in sustainable schemes. And that figure only looks like growing further still, as businesses and funds see eco-friendly and socially ethical practices as being a very effective way to attract capital. Responsible investing is a two-way street, because private investors in such schemes have been enjoying growth that's up by 25% in the last three years.
Ethical Energy
Renewable energy is a huge draw for private investment and it's perhaps the largest of all the sectors attracting huge sums from private individuals in the United States. Power is a high-profile problem, sparking much and frequent political and social debate, worldwide.
It's also a future-issue that's extremely pressing for a planet with an ever-increasing population and an insatiable technological appetite for energy, to power ever more complex machines.
Energy is a very apparent issue for most, and one that's relatively easy to understand. The way in which we harvest and produce energy in the near future has the power to make or to break the planet on which we live.
What takes place in one part of the planet can affect the population of another part of the plant. The task of finding, solving and then building alternative, clean sources of energy is one that the public can quickly get on board with. Add to that a decent return, and it's easy to see why renewable energy projects get private investors interested.
Investing in People
Socially responsible community investment schemes are growing in popularity amongst people that want to 'put something back' whilst they grow savings or prepare for retirement. Such schemes are a great way to make investors feel that they're making a difference.
In modern times, and certainly after the financial crash of 2008, the big banks have been getting a bad press, being viewed by many as institutions that hold too much sway, are not accountable, and are void of any form of social responsibility. Getting involved with socially responsible funds is a very attractive alternative to the big banks, and this seems to be true for a growing number of people.
An investment gesture as simple as depositing a portion of one's savings in a community bank or credit union can have a far-reaching effect and can produce profound and very apparent results in neglected and economically disadvantaged communities.
Credit unions may use invested capital to fund training programs in order to kick start the commercial and industrial viability of an area, through making people more employable. Loans can also be made available to local entrepreneurs – people that often hold slim or zero chance of a start-up loan from a traditional bank, and communities can be transformed relatively quickly by such schemes.
Responsible Research
Options for those that wish to make responsible decisions while investing are many, and the terms involved can cover wide and varied ranges of schemes, business, industry, and education and community projects. It's important, as with any investment, to do your research.
The trend toward all things ethical and sustainable does not look like slowing, and as the Western world becomes a more and more socially responsible place, as our knowledge of the implications of our indirect actions becomes easier to monitor on social media and the like, perhaps it's not so surprising that the greenback has never been greener.
If you want to know more about what green investing is and how you can make some simple eco-friendly swaps to get started, check out this infographic:


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Hi Team,
We have a requirement to retrieve the application name from the incoming client id to validate against an attribute "sourceSystem" present in the payload.
Currently our enterprise Mule version helps us getting the client id details for us however, we don't have any feasibility to know the application name corresponding to the client id.
Please let us know if there is a way we can retrieve the application name for our needs.
Dec 06, 2017 at 04:32 AM, kunalcapg answered with:
Ideally one client Id would be associated to a nos of applications, You can use cloudhub APIs to get the application name please refer to the CLOUDHUB API Portal here Go through the API references it should help you get the application details.
If you use this resources it should work out for you /organizations/{organizationId}/applications/{applicationId}
Thanks Kunal. Although we don't have access to these APIs from our enterprise on-premise version, it is understood that this will provide us the relevant details for us.
Oct 02, 2018 at 08:29 AM, c-a-stefancordes answered with:
Just use
which will result in the application name which was specified during "Request API access".
See https://support.mulesoft.com/s/article/How-to-get-the-client-application-name-in-a-flow-based-on-the-client-id-and-client-secret


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When choosing a real estate professional, get to know their history!
Below, you'll find information that will help you get to know me better - from awards and credentials I've achieved, to references from past clients.
I consider the satisfaction of my clients to be my greatest achievement, and take pride in my high volume of repeat business and referrals.
Call me today - I'd love to add you to this growing list!
We recently moved to Saskatoon from out of province and began our house hunt by checking out open houses which is where we met Alana. We were instantly won over by her approachable and kind personality and ended up continuing our house hunt with her. She was absolutely meticulous and understanding along the way. She was able to give helpful advice about good neighborhoods for our family while sticking to our "wish list" of what we wanted in a home. It is clear that she loves what she does, shared our excitement during the search and was on our side to help us get what we wanted throughout the entire journey. As first time home buyers we were fairly clueless about the entire process of purchasing a home and Alana was so respectful and clear about how things worked and got us in contact with a great mortgage broker too. She always had our back and made things happen quickly and smoothly. We highly recommend using Alana Loth in your home search!Ryan and Jocylene R.
Alana came recommended to us by a trusted friend during a difficult time in our families life. Having recently lost a family member we were looking for a very specific home that would accommodate a list of must haves that meant our options may be somewhat limited. Alana used her knowledge and experience of the market to provide us education on options and the pros/cons for OUR family for building. She was always available for discussions or viewings and while very professional was able to use a positive and cheerful personality in helping us with what could often be considered a stressful time. Once narrowing down our search Alana was able to list our beloved first home and sell it in a timely manner at our asking price. Alana is professional, hard working and is able to relate to her clients on a personal level making the buying/selling process enjoyable and less stressful. She started as our realtor but now is our friend. We highly recommend her.Melissa and Kevin
Alana started working with our family and had the patience over 3 years to make our move happen. Finding a home in our price range and in the area we wanted was a huge undertaking. Alana took us to see so many homes in that time period and made us feel like the perfect home was out there and that we shouldn't give up. When the day came that we found 'the one', we had to quickly purchase and she was there on our lunch hour to assist. We also had to quickly sell. Alana sold our home our first day on the market and made our new dream a reality. We were and are so thankful she stuck by us and love our home so much. Thanks Alana!
Marty and Krista


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Where the Sunshine Is Expensive – Aubrey Logan, Dave Koz & Casey Abrams
© ℗ 2019 Resonance Records
Aubrey O'Day Booted from NYE Party She Hosted in 'Honor' of Donald Trump Jr.
[[tmz:video id="1_a1uy9y4n"]] Aubrey O'Day rang in the New Year by getting kicked out of her own show … at a faux birthday party at a strip club in honor of her alleged ex, Donald Trump Jr. Folks at Larry Flynt's Hustler Club in Las Vegas tell TMZ ……
Aubrey O'Day Hosting New Year's Eve Birthday Bash for Donald Trump Jr.
If Donald Trump Jr. doesn't have plans to celebrate his 41st birthday on NYE, a Vegas strip club is willing to throw him a party … hosted by none other than his alleged ex, Aubrey O'Day. Larry Flynt's Hustler Club in Sin City is extending an…
Ariana Grande's Surprise "Thank U, Next" Release Has Fans Asking "Who's Aubrey?"
Ariana Grande spontaneously released "thank u, next" and Apple Music's lyrics had fans thinking she could be bisexual and dating someone named "Aubrey."
WIRED Autocomplete Interviews – Aubrey Plaza & Craig Robinson Answer the Web's Most Searched Questions
"An Evening with Beverly Luff Linn" stars Aubrey Plaza and Craig Robinson take the WIRED Autocomplete Interview and answer the internet's most searched questions about themselves. Is Aubrey Plaza single? Was Craig Robinson on Friends?
WIRED Videos
Donald Trump Jr.'s Ex-Mistress, Aubrey O'Day, Poses Half Naked for Easter
Aubrey O'Day's on a roll for Easter, but it ain't like the one that's happening at the White House this week … not even close, actually. You seeing this, DTJ? Aubrey posted a revealing photo Sunday just in time for Easter, which shows her bottomless…
Aubrey O'Day Surfaces, Tight-Lipped on Donald Trump Jr. Affair
[[tmz:video id="0_813d1uiu"]] Aubrey O'Day is finally returning to going out in public — on the heels of her alleged Donald Trump Jr. affair becoming huge news – but she's definitely not pulling a Stormy Daniels. Yet. The singer, and…
Aubrey O'Day Porn Search Popularity Soars After Alleged Donald Trump Jr. Affair
If Aubrey O'Day is looking for a new career move, she should seriously consider doing porn. Pornhub, one of the biggest porn sites out there, tells us searches for O'Day's name on their site rose a whopping 10,405% after news broke of her alleged affair…
Aubrey O'Day: I Had a Sexual Relationship with Donald Trump Jr.
Aubrey O'Day is telling close friends she absolutely had a sexual relationship with Donald Trump Jr. for months, but she strongly believes it has nothing to do with Trump's divorce. Sources closely connected to O'Day tell TMZ, she had an affair with…
Aubrey O'Day's Topless Unreleased Music Vid Allegedly Aimed at Donald Trump Jr.
[[tmz:video id="0_plsnrlbm"]] Aubrey O'Day wrote not one, but 2 songs about her alleged affair with Donald Trump Jr. – and even made a steamy music vid for one of them, but it never saw the light of day … until now. TMZ's obtained previously…
Impossible – Aubrey Logan
© ℗ 2017 Knight Music
Impossible – Aubrey Logan
© ℗ 2017 Knight Music
Give It Away (feat. Aubrey Logan) – Single – Scott Bradlee's Postmodern Jukebox
iTunes 100 New Releases
Aubrey O'Day Judges Twerking Auditions (And The Competition Is FIERCE)
Aubrey O'Day joins Todrick Hall in his new music video, "Dem Cakes Tho."
Stefanie Scott & Aubrey Peeples On Bringing 'Jem' To The Big
Stefanie Scott and Aubrey Peeples talk to Access about their roles in the film version of the 1980's TV series, 'Jem and the Holograms.'
Access Hollywood Latest Videos
Aubrey Plaza, Paul Scheer and Rob Huebel – The Bible Rules – @midnight with Chris Hardwick
Aubrey Plaza, Paul Scheer and Rob Hue… 2:03
Inspired my Chris's misreading of a Netflix series's title, Aubrey Plaza, Paul Scheer and Rob Huebel preach the lessons of the Gospel According to Awesome.
Watch full episodes of @midnight now: http://on.cc.com/17MOT5T
Submitted by: @midnight
Keywords: Chris Hardwick @midnight at midnight After Midnight Nerdist @midnight full episodes Aubrey Plaza Paul Scheer Rob Huebel Netflix Bible marijuana drugs sexual advances comedy central stand up comedy comedians comedy central comedians comedy funny comedian funny video comedy videos stand up videos funny jokes funny clips hilarious videos hilarious clips
Views: 835
Funny or Die | Funny Videos, Funny Video Clips, Funny Pictures
#NewHeat: Prince Rick & Aubrey Mikel – "No Fool" (Official Music Video)
The Wait is Over!!…How Could Prince Rick Not Drop A New Song & A New Music Video On His Birthday with Aubrey Mikel Check It Out! #NOFOOL #August5th #HereForever
Filed under: Multimedia / Video, Music Tagged: #HeaterOfTheDay, Aubrey Mikel, hip hop, music, No Fool, Prince Rick
Aubrey Morris, 'A Clockwork Orange' Actor, Dies at 89
He also had roles in 'The Wicker Man,' 'Lisztomania' and 'Love and Death.'
Aubrey O'Day — You're Gonna Love My New Pair … Danity's Dead to Me
Aubrey O'Day is officially putting Danity Kane in her rear view mirror, and all it took was hooking up with another hot … yet familiar blonde to form a new band. Sources close to Aubrey tell us she and ex DK'er Shannon Bex are moving on as a…
Ass Stuffed Aubrey Addams
Cute babe Audrey Addams has her tight ass finger fucked before having it stuffed with cock. Click here to watch the movies.
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At Any Price – Brenna Aubrey
Jem and the Holograms Star Aubrey Peeples Teases Movie's Music, Fashion and Possible Sequel
Aubrey Peeples may be playing Jem in Jem and the Holograms, but she isn't exactly a longtime fan of the 1980s cartoon series.
"I can't lie, I didn't know about it…
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Aubrey Addams, Talon
Hot blond co-ed Aubrey Addams has a sweet round ass and loves to have it shiny and glistening in the light. She walks upstairs into the house and crawls onto the waiting bed arching her back like a cat and shaking her butt. Talon is eager to get his hands on her, bends her over and shoves his fingers into her juicy hole, then pulls her to his waiting boner so she can lube it up with her warm mouth. Gripping her hips he pulls her wet snatch to his the tip of his cock and slides it deep inside her with one hard thrust then keeps pounding her until she cums and he is ready to blow his load in her open mouth.
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To see more join Foxmagazine.com right now!


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Today our task is to create a pictogram of an olympic sport using Otl Aicher's 45 degree pictogram grid.
Using only shape and colour, I also need to consider:
- Audience
- Scale
- Contrast
- Impact
- Clarity
To begin my research I looked at the pictograms of the olympic summer games from Tokyo 1964 to Rio 2016.
Mexico City 1968
Designers: Urban Design Department of the Organising Committee of the Games of
the XIX Olympiad, including Lance Wyman.
Within this design it only uses part of the athlete's body or the equipment used which makes it really distinct and visually interesting. This helps to make the sport non gender specific and really highlights the sport it is portraying. The reference to Mexican culture and history helps contextualise and make it more appropriate for where it is being used while still being a universal design. Considering this was for the 1968 games it uses a very modern approach of simplistic shapes and bright colours that evoke joy and excitement. This is also the only olympic pictogram design which does not include whole figures, yet the sports are still reasonably clear.
Looking at the rest of the designs it ranges from more detailed symbols of figures to a more abstract design working with line and stroke.
Tokyo 1964
Munich 1972
Montreal 1976
Moscow 1980
Los Angeles 1984
Seoul 1988
Barcelona 1992
Atlanta 1996
Sydney 2000
Athens 2004
Beijing 2008
London 2012
Rio 2016
For this task I have chosen to portray the sport sailing which has been part of the Olymipics since 1988. I personally really enjoy sailing and have taken part in it a few times so because it is a sport I enjoy I thought it appropriate to design a pictogram for it.
Otl Aicher created pictograms for the 1972 olympic games in Munich. He revolutionised visual communication by creating the 45 degree grid. This helps to keep consistency and clarity, they facilitate international understanding.
I began by looking at some sailing pictures online and doing some quick rough sketches from these:
I then moved on to applying the grid and working with different designs to see which produced the most clear and understandable symbol:
I noticed that having a figure helped to imply action which gave greater clarity to what is happening in the image. I found using the grid to be quite annoying as I wanted to move beyond its restrictions, however I do see the benefit of when designing a set they all adhere to the same sort of design.
This is the design that I have come up with for sailing using Otl Aicher's grid:
Within this design it show's clarity, as it very obvious what it is, this is very important when trying to engage with an international audience. Due to its simplistic lines this pictogram would work well at different scales and would still be recognisable. The block shape of the figure contrasting with the outline of the boat creates this sense of power in the figure, the one performing the sport.


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Oldfield Smith have available to let Unit 6 at Firgrove Business Park, Cross-in-Hand situated along Firgrove Road a short distance from its junction with B2102 Lewes/Blackboys Road.
There are a number of businesses located at the park including outside caterers Food glorious food. Units 6 & 7 are located to the rear of the development and comprise detached workshop having 2 separate units. Each unit has electric roller shutter door to front and cloakroom. Unit 6 has a further door to rear currently sealed internally.
Unit 6 – internal width 11.9 m (39′) and depth 11.6m (38′). The eaves height is 4.2 m (13′ 9″) and Apex 5.3m (17′ 3″)
Gross internal area 138 sq m (1482 sq ft)
within this space has been created an office/kitchen area fitted with stainless steel sink unit and cloakroom with low level wc and wash hand basin. Parking is provided to the front and rear of the building.
New lease available on normal full repairing and insuring basis, length to be agreed.
For further information please click here or for an appointment to view please call 01825 762222.


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An online resource center providing you with additional helpful information.
View the KidsDoc Symptom Checker from HealthyChildren.org
School's out! Time for the Family Vacation. So how do you have fun without going insane? I ,of course, have some suggestions:
10. Pack a simple medicine kit: don't waste a day of vacation at the doctor's office, refilling the prescription you forgot at home. Take:
9. Write out a budget before you go. I know, I am a fun sucker, but it has to be done. Know how much money you have and where you plan to spend it. Give the kids an allowance for souvenirs. They will be more careful with money they consider their own, and they will not be constantly asking for things. "Can I have that?" can be answered with "Sure, it's your money. But are you positive that is where you want to spend it? There might be something better later…" Also, knowing how much you yourself have to spend will save you stress and regret later.
8. Keep to healthy foods most of the time. (Here I go, sucking out the fun again!) Kids will have more energy, feel better and have a better attitude if they are nourished. And it's cheaper. Have a basket of fruit available, some whole grain crackers, cheese, peanut butter, popcorn – food with nutrients. Think of all the drive thru's and arguments you will avoid!
7. Keep to established routines when you can. Bring along a book for that bedtime story, keep bed time the same, set aside time for their bath. Kids don't always deal well with change, and vacations are all about change. A few familiar routines will help them feel less stressed. And a full night's sleep is an absolute necessity if you don't want an emotional wreck for a kid.
6. Keep an eye on the little ones. You are in a different environment with new dangers. Distractions abound. Kids on vacation get lost, or get into Grandma's meds or the neighbor's pool.
5. Find interesting things to keep their brains busy. Bored kids whine, and then they find their own version of interesting things. Have a stock of books, games and videos for the car. Bring a journal for them to write in, and art supplies. Explore the area you travel to – Google it before you go. See the sights, hit the museums, find the local artists and craftsmen.
4. Keep your mind open to new and different ways of doing things, so that your kids will do the same. Kids internalize their parent's judgments, and they will close down their minds and wipe possibilities out of their lives if that is the example you set.
3. Keep them physically active as well. A tired kid is less stressed, sleeps better, and is not sitting around thinking of ways to get into trouble.
2. Keep stress to a minimum. Use a GPS if you're driving: arguments with the navigator have ruined many a vacation. Keep your expectations in line with the actual possibilities, to avoid disapointment. Don't overschedule – leave time for that relaxing hike and to have a conversation over dinner. Stay within your budget – your hindbrain will know you are overspending and your stress will mount. Stressed out people snap at each other and cannot enjoy their time or their families.
1. Align your vacation with your priorities, then toss out the rest. What are the goals of this vacation? Relaxation, family time, memories, enrichment, joy? Plan the vacation and activities that will get you there, and don't let exhaustion, stress, and fear get in your way. Don't stop at Uncle Joe's house if you know he will stress you out; don't vacation with those friends who overspend or forget to pay their half of the bill. Just say no.
Have a fabulous vacation!
Kathleen Lovlie, MD
Lee Autrey, CRNP
232 Office Park Drive
Gulf Shores, AL 36542
Questions or Comments?
We encourage you to contact us whenever you have an interest about our services.


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Ever since San Diego Comic-Con back in July, rumours have been spread that Ben Affleck is set to direct and star in a solo Batman film, following his appearance in Batman v Superman: Dawn of Justice.
It was reported that Affleck and Geoff Johns were working on a screenplay together and in a recent interview with Total Film (via Heroic Hollywood), the Oscar-winning Argo director said it would be like a dream to direct such a project.
"Look, if I could make it work, yeah, obviously it would be like a dream. The trick is, as to any movie, is to make sure that you- if you're going to direct it- that you can make it good. You've got to have the right idea and the right take on it. Broadly speaking, it's something I'd be open to, for sure. It's a very exciting idea."
Zack Snyder says there's a Batman inside all of us—but Ben Affleck's will be "fabric-based." https://t.co/Vykx9tTQ9R pic.twitter.com/SWh8L2zFHz
— Collider News (@ColliderNews) October 22, 2015
However, producer Charles Roven echoed Affleck's concerns about a hectic schedule, meaning that we may not see a standalone Batman flick for at least a number of years. +
"He's got a busy schedule, he's about to start a movie now. And he's also got obligations to be in the Justice League movie. The calendar is a little bit tricky. Of course, the script obviously has to be worthy of him wanting to direct it, and once that happens, it's still got to fit into the calendar. We're hoping we can make it happen. We're just at the beginning of the question."


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