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Author Affiliations
- Correspondence: Paul Proost, Laboratory of Molecular Immunology, Rega Institute, K.U.Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium. E-mail:paul.proost{at}rega.kuleuven.be
Background During the first line defense of an infected host, circulating neutrophils invade the inflamed tissue, whereas mature neutrophils from the bone marrow pool migrate into the blood circulation and from there reinforce tissue infiltration. The CXC chemokine CXCL8, also know as interleukin-8, is a potent attractant of neutrophils. Recently, we discovered a new natural post-translational modification of CXCL8, i.e. the deimination of arginine into citrulline by peptidylarginine deiminases.
Design and Methods The ability to provoke leukocytosis was assessed by intravenous administration of citrullinated CXCL8 in rabbits. Adsorption of citrullinated CXCL8 to the Duffy antigen/receptor for chemokines on human or rabbit erythrocytes was evaluated using a competitive binding assay. Finally, surface expression of adhesion molecules was studied after stimulating neutrophils with citrullinated CXCL8.
Results Citrullination of CXCL8 significantly increased this chemokine's ability to recruit neutrophils into the blood circulation. In addition, the competitive binding properties of CXCL8 for the Duffy antigen/receptor for chemokines were impaired upon citrullination. Since the Duffy antigen/receptor for chemokines is an important scavenging receptor for CXCL8 in the blood stream, citrullination may delay CXCL8 clearance from the circulation. Furthermore, the shedding of CD62L (L-selectin) and the upregulation of CD11b (β2-integrin) protein expression on CXCL8-induced neutrophils were improved by deimination of CXCL8, possibly contributing to the neutrophil egress from the bone marrow. Conversely, surface expression of CD15, the neutrophilic ligand of endothelial selectins, was equally well upregulated by intact and citrullinated CXCL8.
Conclusions These data show that citrullination of CXCL8 enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation and prolonged presentation to the bone marrow.
Chemokines are small chemotactic cytokines involved in inflammation-induced leukocyte recruitment, tumor development, angiogenesis as well as homeostasis.1 Based on the conserved pattern of Cys residues, chemokines are classified into four groups, i.e. C, CC, CXC and CX3C chemokines.2,3 CXCL8 (also known as interleukin-8) is an inflammatory CXC chemokine that contains an ELR motif (Glu-Leu-Arg) in front of the most NH2-terminal Cys residue. CXCL8 preferentially targets neutrophils via two CXCL8 receptors, CXC chemokine receptor (CXCR) 1 and CXCR2, both members of a seven transmembrane spanning G protein-coupled receptor superfamily.4 Presentation of chemokines on the surface of endothelial cell layers to neutrophils in blood is achieved through the interaction with glycosaminoglycans (GAG).5 In addition, CXCL8 can be adsorbed by the Duffy blood group antigen/receptor for chemokines (DARC) present on erythrocytes and vascular endothelium, restricting its capacity to stimulate neutrophils in the blood flow.6–8
Post-translational modifications of chemokines constitute an important regulatory pathway in the immune response, since structural alterations can profoundly affect the biological characteristics of chemokines.9 Partial NH2-terminal proteolysis of CXCL8 into CXCL8(6–77) potentiates its in vitro and in vivo neutrophil chemotactic properties 3- to 10-fold, whereas truncation in or beyond the ELR (Glu-Leu-Arg) motif abolishes its biological functions.10–14 Recently, our group discovered a new post-translational modification of chemokines, i.e. the deimination of arginine (Arg) into citrulline (Cit).14,15 Indeed, a naturally citrullinated CXCL8 isoform was isolated and identified in which Arg at position 5 was converted into Cit, designated CXCL8(1–77)Cit5. Peptidylarginine deiminase (PAD) 2 and 4 efficiently and specifically modify Arg5 in CXCL8. CXCL8 citrullination only moderately alters its in vitro activities, but resulted in a considerable reduction of glycosaminoglycan binding properties. In addition, citrullination of CXCL8 prevented proteolysis by plasmin or thrombin into CXCL8(6–77). Furthermore, this modification of CXCL8 abrogated the chemokine's in vivo capacity to recruit neutrophils into the peritoneal cavity, whereas angio-genic properties were retained.14
Chemokines (e.g. CXCL8) influence mobilization of leukocytes from the bone marrow into blood.16–20 In complementation of our recent studies, we evaluated the effect of CXCL8 citrullination on the recruitment of neutrophils into the blood vessels by intravenous administration of different CXCL8 isoforms. In addition, further explanation is provided on the previously documented decreased intraperitoneal recruiting potency of citrullinated CXCL8.14 Finally, the effect of chemokine citrullination on the regulated expression of adhesion molecules was assessed.
Design and Methods
Citrullination of chemokines
Human recombinant CXCL8(1–77) was purchased from PeproTech Inc. (Rocky Hill, NJ, USA). Citrullinated CXCL8 was obtained by incubation of CXCL8(1–77) with rabbit skeletal muscle PAD (Sigma-Adrich, St. Louis, MO, USA) in 40 mM Tris-HCl, pH 7.4, 2 mM CaCl2 at 37°C for 90 min at an enzyme-substrate ratio of 1:200.14 Deimination was stopped with 0.1% TFA and citrullinated protein was purified on a C8 Aquapore RP-300 HPLC column (1×50 mm, PerkinElmer, Norwalk, CT, USA). Endotoxin concentrations were evaluated with the Limulus amoebocyte lysate test (Cambrex, East Rutherford, NJ, USA).
Leukocytosis assays
Induction of leukocytosis was measured in New Zealand white rabbits by i.v. injection (1 mL) of 10 μg of chemokine in phosphate-buffered saline (PBS).19 Blood samples were collected from a peripheral ear vein into potassium EDTA-coated tubes at several time intervals before and after treatment. Total leukocytes were counted in triplicate and the percentage of granulocytes was determined in quadruplicate by 100-cell differential cell counts on blood smears stained with Hemacolor solutions. All animal studies were approved by the review board of the ethical committee of the K.U.Leuven and experiments were performed according to Belgian and European legislation, including the Helsinki declaration. Statistical analyses were performed using the Mann-Whitney test on paired values.
Isolation of neutrophils and erythrocytes
Freshly isolated buffy coats (Blood Transfusion Center, Red Cross Leuven, Belgium) were subjected to sedimentation in hydroxyethyl-starch (Plasmasteril, Fresenius, Bad Homburg, Germany) and subsequently to gradient centrifugation using Ficoll-sodium metrizoate (Lymphoprep, Nycomed, Oslo, Norway).21 Alternatively, rabbit blood, collected from ear veins, was subjected to gradient centrifugation using Nycodenz solution (NycoPrep, Nycomed).22 Neutrophils recovered from the pellet were subjected to hypotonic shock and washed twice in PBS before use in binding assays. Alternatively, human blood withdrawn from a healthy donor or rabbit blood from ear veins was centrifuged at 125 g at 4°C for 10 min. Plasma and buffy coat were removed by aspiration, and erythrocytes were washed twice with PBS.
Binding competition assay
Competition for 125I-labeled CXCL8(6–77) binding was measured on freshly isolated neutrophils or erythrocytes in binding buffer [50 mM HEPES pH 7.2 containing 1 mM CaCl2, 5 mM MgCl2 and 0.1% (w/v) bovine serum albumin]. Briefly, 2×106 neutrophils or 2×108 erythrocytes were incubated for 2 h at 4 °C with 125I-CXCL8(6–77) (PerkinElmer) and unlabeled chemokine. Cells were centrifuged and washed three times with 2 mL of binding buffer supplemented with 0.5 M NaCl and the radioactivity was measured in a γ counter (Triathler Multilabel Tester, Hidex, Finland). Statistical analyses were performed using the Mann-Whitney test on paired values. IC50 values were calculated by performing linear regression on the descending part of the curves of individual experiments.
Adhesion molecule staining in peripheral blood
Blood samples from healthy volunteers were collected by venipuncture, using a 21 G needle, into lithium heparin-treated tubes (Vacutainer, BD Biosciences, San Jose, CA, USA). Blood samples were handled with care in order not to activate the cells and immediately diluted in warm PBS (37°C) to 106 leukocytes/mL in sterile tubes containing prewarmed dilutions of chemokines or fMLP (Sigma-Aldrich.). After incubation for 10 min at 37°C in the presence of 5% CO2, the samples were immediately placed on ice and three volumes of ice-cold PBS were added to the tubes to stop the signal transduction cascades at once. The Fc-receptors were blocked by storing the samples in ice-cold PBS containing 2% fetal calf serum (FCS) for 15 min, prior to staining for flow-assisted cell sorting (FACS). Antibodies used for staining were purchased from BD Biosciences (anti-human CD16 labeled with phycoerythrin, CD11b labeled with CyChrome) or from eBioscience (San Diego, CA, USA) (CD15 labeled with fluorescein isoth-iocyanate, CD62L labeled with allophycocyanin). Subsequently, red blood cells were lysed after washing the cells three times with PBS enriched with 2% FCS and 15 min of fixation with PBS containing 2% FCS and 2% formaldehyde. FACS analysis and data acquisition were performed with a BD FACSCalibur cytometer (BD Biosciences) using CellQuest software (BD Biosciences). Neutrophils were first selected by side/forward scatter and subsequently by CD16 positivity. Statistical analyses were performed using the Mann-Whitney test on paired values.
Citrullinated CXCL8 has enhanced granulocytosis-inducing capacity
Intravenous administration of CXCL8 is known to result in a rapid decrease of the number of circulating granulocytes followed by granulocytosis.19,20 The effects of recombinant CXCL8(1–77) and its citrullinated isoform CXCL8(1–77)Cit5 were compared following i.v. injections into rabbits at doses of 3 μg/kg. The numbers of circulating granulocytes and total leukocytes were determined at different time points (Figure 1). Interestingly, at identical doses CXCL8(1–77)Cit5 induced a significantly more profound granulocytosis than CXCL8(1–77). Although injection of CXCL8(1–77)Cit5 provoked a comparable drop in granulocyte concentration after 15 min, the increase of circulating granulocytes at 2 h post-injection was significantly greater with CXCL8(1–77)Cit5 than with CXCL8(1–77). Moreover, this enhanced granulocytosis induced by CXCL8(1–77)Cit5 remained significantly more pronounced until at least 8 h post-injection compared to that produced by CXCL8(1–77). After 24 h, the number of circulating granulocytes returned to baseline levels for both CXCL8(1–77) and CXCL8(1–77)Cit5.
Citrullination reduces scavenging of CXCL8 by erythrocytes
In order to better understand the differences between intact and citrullinated CXCL8 at inducing granulocytosis in vivo, the cell-binding capacities of these two CXCL8 isoforms were compared in vitro. Freshly isolated human neutrophil granulocytes were used to assess competitive binding with 125I-CXCL8. Interestingly, compared to intact CXCL8, citrullinated CXCL8 showed a modest but significant decrease in competition for 125I-CXCL8 for chemokine receptor binding on human neutrophils (Figure 2A). Since the leukocytosis experiments were carried out in rabbits, binding experiments were also performed on freshly isolated rabbit neutrophils. Again, citrullination of CXCL8 moderately reduced the chemokine's competition for neutrophil receptors (Figure 2B).
An alternative pathway for chemokine clearance from the blood circulation is scavenging by chemokine interceptors.7 Indeed, the majority of circulating CXCL8 is adsorbed by DARC present on erythrocytes, affecting the chemokine's capacity to stimulate neutrophils in the circulation.6,7 To examine this option, the two CXCL8 isoforms were subjected to a receptor competition assay with 125I-CXCL8 on freshly isolated human erythrocytes. Remarkably, over a wide range of concentrations citrullinated CXCL8(1–77)Cit5 was significantly less effective than CXCL8(1–77) on erythrocytes at displacing labeled CXCL8 (Figure 2C). These results suggest that citrullination reduces binding of CXCL8 to DARC. As a positive control, monocyte chemotactic protein-1 (MCP-1/CCL2) efficiently displaced 125I-CXCL8 from red blood cells (Figure 2C and 2D).23,24 In addition, citrullination of CXCL8 also significantly diminished its potency to compete for DARC on rabbit erythrocytes (Figure 2D).
Effect of citrullination on the expression of adhesion molecules on human neutrophils in peripheral blood
Transcellular and intercellular migration of neutrophils across the endothelium requires selectin and integrin activation.25–27 The effects of CXCL8(1–77) and CXCL8(1–77)Cit5 on the neutrophilic expression of adhesion molecules were determined after whole blood stimulation (Figures 3–5).
First, the surface expression of sialyl Lewis X/CD15, the moiety that interacts with endothelial selectins, was evaluated. Both CXCL8(1–77) and CXCL8(1–77)Cit5 caused an upregulation of the neutrophilic expression of CD15 (Figure 3). However, no significant difference in CD15 expression levels on neutrophils was observed between the two CXCL8 isoforms.
Next, the shedding of L-selectin (CD62L) and upregulation of integrins (CD11b) on CXCL8-activated neutrophils were investigated. In contrast with CD15 upregulation, these processes were improved by citrullination of CXCL8 (Figures 4 and 5). Although the CXCL8 isoforms were less efficient than bacterial formyl peptide fMLP, 100 nM CXCL8(1–77) provoked a significant decrease of CD62L expression (Figure 4). CXCL8(1–77)Cit5 already significantly enhanced shedding of CD62L on neutrophils at a concentration of 30 nM. Besides, 3 nM CXCL8(1–77)Cit5 notably increased the CD16/CD11b+/+ population, whereas CXCL8(1–77) only induced a significant rise of CD11b expression from concentrations of 30 nM upwards (Figure 5). These results indicate that citrullinated CXCL8 is 3 to 10 times more potent than intact CXCL8 in causing CD62L shedding and CD11b upregulation.
The bone marrow is a source of hematopoietic stem and progenitor cells able to differentiate into myeloid and lymphoid cell lineages. Neutrophil granulocytes are not only produced in the bone marrow, but are also stored there in a mature form known as the bone marrow pool. Upon inflammation, these mature granulocytes are rapidly mobilized into the blood stream to reinforce the first line defense of circulating neutrophils, a process termed leukocytosis. Therefore, the clearance of chemokines from the blood circulation and the rate of neutrophil intravasation over the bone marrow endothelium are essential factors affecting the number of circulating neutrophils.28,29 Furthermore, the potency of bone marrow neutrophils in responding to chemoattractants, phagocytosis and bacterial eradication is greater than that of circulating neutrophils.30 Strong granulocytosis is provoked within hours by most CXC chemokines, including CXCL8.16–20 Here, an even higher neutrophil mobilization into the blood stream was evidenced upon i.v. administration of citrullinated CXCL8 in rabbits. In addition, we observed that erythrocyte binding was severely decreased for CXCL8(1–77)Cit5, in both humans and rabbits. Since the clearance of CXCL8 from the blood circulation is predominantly due to binding to DARC on erythrocytes,6,7 this impairment could result in a longer presentation of the chemokine to the bone marrow and, hence, enhanced neutrophilia. In previously reported studies, we observed that citrullination of CXCL8 at Arg5 prevents the chemokine's truncation by plasmin or thrombin into the more potent CXCL8(6–77) isoform.14 Furthermore, the reduced GAG binding capacity of citrullinated CXCL8 may impair its immobilization on the vessel wall, which is necessary for transcytosis of leukocytes to the luminal side of endothelial cells.31
In conclusion, all these data suggest that citrullination of CXCL8 reduces the clearance of this chemokine from the blood circulation. These results may provide an explanation for the previously documented deficient recruitment of neutrophils from the blood circulation towards inflamed tissue.14,15 Indeed, intra-peritoneal administration of CXCL8(1–77)Cit5 in mice did not result in an infiltration of the peritoneal cavity by neutrophils, whereas intact CXCL8(1–77) and truncated CXCL8(6–77) were highly potent at mobilizing neutrophils from the blood circulation. This insufficient pro-inflammatory response after citrullination indicates an inherent protective role for PAD during local inflammation but may also guarantee a constant mobilization of neutrophils from the bone marrow to keep cell numbers equivalent in the circulation after extravasation into inflamed tissue.
Although PAD is normally expressed in the cytoplasm and occasionally in the nucleus, extracellular localization of PAD2 and PAD4 from infiltrated leukocytes has been detected in inflamed synovial tissue.32–35 When the citrullinated chemokines appear in the blood flow, they may contribute to the development of generalized inflammation.
Leukocyte mobilization into the circulation and recruitment into tissues requires upregulation and activation of adhesion molecules.25–27 Neutrophils express CD15 or Lewis-X sialic acid, which binds selectins on endothelial cells. In this study, no increase in the upregulation of CD15 was detected after citrullination of CXCL8. In contrast to extravasation from blood vessels into the interstitium that occurs through cell-cell junctions, mobilization from the bone marrow seems to involve transcytosis through pores in the endothelial cells.36 Bone marrow neutrophils do not appear to require tethering on the endothelium, although L-selectin/CD62L is significantly downregulated upon inflammation, suggesting that CD62L acts as a retention factor, in analogy with its lymphocyte homing function in lymph nodes.37,38 In this study, it was found that CD62L was shed when neutrophils were stimulated with citrullinated CXCL8. In fact, citrullination converted CXCL8(1–77) into a significantly more potent down-modulator of CD62L. After binding of the chemokines to their appropriate receptors, several intracellular cascades are activated, including upregulation and activation of integrins, which are implicated in the immobilization of leukocytes on the vessel wall.39 The expression of β2-integrin (CD11b) on neutrophils was found to be improved after citrullination of CXCL8(1–77). Thus, citrullination of CXCL8 prolongs the presence of the chemokine in the circulation and increases the shedding of CD62L and upregulation of CD11b expression. Such processes may contribute to the sustained intravasation of neutrophils from the bone marrow through the sinusoidal endothelium into the circulation.
Overall, these data shed light on the greater capacity of CXCL8(1–77)Cit5 to recruit neutrophils from the bone marrow. Indeed, it may be valuable to explore the use of citrullinated chemokines as an additional clinical approach to mobilize progenitor cells into the blood. Growth-related oncogen-β (GROβ/CXCL2), another CXCR2 agonist, synergizes with granulocyte colony-stimulating factor in mobilizing hematopoietic progenitor cells from the bone marrow.40–43 Interestingly, citrullination is a natural and irreversible reaction and we showed that it protects CXCL8 against proteolysis.14,44 Furthermore, significantly elevated leukocytosis was obtained by citrullinating CXCL8. Thus, these data suggest that citrullination of CXCL8 or other chemokines could be a potential mechanism to assist granulocyte colony-stimulating factor in the recruitment of hematopoietic cells from the bone marrow into the blood.
the authors thank René Conings, Mieke Gouwy, Jean-Pierre Lenaerts, Willy Put, Isabelle Ronsse, Chris Dillen, Evemie Schutyser and Sofie Struyf for their technical assistance and Anneleen Mortier for critically reading the manuscript.
Authorship and Disclosures
TL, GO and PP performed the experiments; TL and PP collected and analyzed the data; TL, GO, JVD and PP designed the research and wrote the manuscript. All authors approved the final version of the manuscript and declare that they have no conflicts of interest.
Funding: this work was supported by the Center of Excellence (Credit no. EF/05/15) of the K.U.Leuven, the Concerted Research Actions (G.O.A.) of the Regional Government of Flanders, the Fund for Scientific Research of Flanders (F.W.O.-Vlaanderen), the Interuniversity Attraction Poles Programme (I.U.A.P.) – Belgian State –Belgian Science Policy, and the European Union 6FP EC contract INNOCHEM (grant LSHB-CT-2005-518167).
- Received February 5, 2009.
- Revision received April 30, 2009.
- Accepted April 30, 2009.
- Copyright© Ferrata Storti Foundation

subset: commoncrawl


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The final numbers are in for the top 30 Switch eShop titles in July. We bet you can't guess the number one….yep…that was sarcasm. Some of them are of no surprise but we are quite happy to see Breath of the Wild still rockin' it at number 6! Without further ado, here is the July top 30:
1. Fortnite
2. Octopath Traveler
3. Hollow Knight
4. Minecraft
5. Enter the Gungeon
6. Zelda: Breath of the Wild
7. Splatoon 2
8. Sonic Mania
9. Mario Tennis Aces
10. Rocket League
11. Stardew Valley
12. Crash Bandicoot N. Sane Trilogy
13. Mega Man X Legacy Collection
14. Captain Toad: Treasure Tracker
15. Mario Kart 8 Deluxe
16. Fallout Shelter
17. Arcade Archives Vs. Super Mario Bros.
18. Dead Cells
19. Overcooked: Special Edition
20. Pokemon Quest
22. Paladins
23. Mega Man X Legacy Collection 2
24. Super Mario Odyssey
25. Pocket Rumble
26. Mario + Rabbids Kingdom Battle
27. Wizard of Legend
28. Ys VIII
29. Xenoblade Chronicles 2
30. Overcooked 2
Which of these titles do you currently own? Which do you still plan to pick up? Let us know in the comments below or chat at us in our Community forum!
SourceCode, aka Lucas Hughes, is the creator/owner of Nintendo Playroom. He spends his days primarily as a husband/father/programmer/gamer. He loves all things Nintendo and is very passionate about the Nintendo community.


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T minus 1 Year from the 50th Anniversary Display of Neil Armstrong's Spacesuit: The Mannequin & Display Case Take Shape
The Secrets that Lie in the Interior Layers of Neil Armstrong's Spacesuit/Call for Artists: Apollo 11 Commemorative Coin
Hey, Houston (and St. Louis, Pittsburgh & Seattle!): The Apollo 11 Command Module Columbia Will Be Landing Near You!
Smithsonian Has Launched Its Second Kickstarter!
Hello team #RebootTheSuit!
Last year, the Smithsonian took you to the moon and back. Now, let us take you over the rainbow!
Yesterday, we launched our second Kickstarter to Conserve... Read more
Armstrong Gloves and Helmet on Display for Moon Landing Anniversary
Time flies! Tomorrow, July 20th, 2016, is the 47th anniversary of the Apollo 11 Moon landing. It's also the anniversary of our Kickstarter campaign to #RebootTheSuit. It's hard to believe... Read more
3D Scanning Neil Armstrong's Gloves
Dear Reboot the Suit Backers,
We hope you've been enjoying our updates about Neil Armstrong's EVA gloves. It's been great to share with you interesting features about the glove, materials... Read more
The Inside Scoop on Neil Armstrong's EVA Gloves
Happy Spring, "Reboot the Suit" backers!
This month, we wanted to get all the details on Neil Armstrong's EVA gloves. One way to do this is with x-rays. As you... Read more
Probing the Hidden Secrets of the Apollo Glove
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How Do You Display a Spacesuit?
Wow, it's 2016! Work has started on the suit, and we're excited about sharing a brand new year of discoveries with you. Thanks for helping us start the work of... Read more
December Tidings: More on 40-Year-Old Spacesuit Documents/Rewards Update/Backers Behind-the-Scenes
In the October update, we told you about an ILC Dover employee who held onto paperwork about Neil Armstrong's spacesuit for 40 years.... Read more
NOVEMBER NEWSFLASH: Alan Eustace Drops In and Rewards in Orbit!
From Neil Armstrong to Alan Eustace: Has Spacesuit Technology Changed after 45 Years?
It's been an exciting November! Alan Eustace, who on October 24, 2014 set a new world record... Read more
A Cool October Surprise: 40-year-old Spacesuit Documents Resurface
These past few months have been a whirlwind of activity! Your generosity and support in exceeding our stretch goal in August overwhelmed us. We appreciate that over 9,000 backers believed... Read more
Thank you to each and every one of you! 9,477 of you joined us on the amazing journey to #RebootTheSuit. Throughout the past 30 days, we've been moved by your... Read more
$700,000 Stretch Goal Reached at T-17 Hours!
Thanks to ALL of you, WE did it! $700,000 reached with 17 hours to go! We hope you are as proud as we are... Read more
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With less than 40 hours left to get to $700,000, Optimus Prime, commander of the Autobots, is leading the charge. Help us... Read more
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Less than 5 days remain to get to our $700K stretch goal! Thanks for getting us light years ahead, but we've got just about $40K more... Read more
New Reward Tier/From Mercury to Apollo/Mission Patch Update/Cool Kickstarter Campaigns for $500
It's wonderful that you love our rewards, and yes, we've been hearing your great feedback about add-ons. We've looked into it but unfortunately, we're not... Read more
Countdown is On!/Sharing Your Apollo 11 Stories/Throwback Tuesday/Optimus Prime Reward
The Countdown is On!
With 7 days and 13 hours remaining, we are less than $65,000 from our $700,000 stretch goal. If you haven't made your pledge yet, there's still... Read more
What's in a Spacesuit Factory? Behind-the-Scenes at ILC Dover/More on Spacesuits
This past Wednesday's Reddit AMA with our curator Cathleen Lewis and conservator Lisa Young showed us just how incredibly passionate and curious you all are about spacesuits!
Neil Armstrong's spacesuit... Read more
Happy Birthday, Neil Armstrong!/Thank You Bruce R. McCaw and Family/Reddit AMA Today 1-3pm EDT
August 5, 1930 - August 25, 2012
Neil Armstrong was the first man on the Moon and the first to have his spacesuit Kickstarted by YOU!... Read more
Mission Patches/Reddit AMA Tomorrow
Mission patches are a really interesting art form. They capture the imagination, the significance and the excitement of something that represents the work of thousands of people, including... Read more
Tell Your Friends: Reboot the Suit Today and Double Your Impact!/ About Boots and Suits
Thanks, everyone, for your great response these past few days. You told your friends about #RebootTheSuit and we're so grateful. We're on an awesome trajectory towards... Read more
Halfway Through Our Stretch Goal!
Epic News!
We've reached the $600K mark! That's $100K more than our original goal of $500K and half-way to our "stretch" goal of $700K. Our target to conserve, digitize and... Read more
Chris Hadfield & Adam Savage Message/What's Next for the Suit/A Small Favor/Featured Rewards
Hello, Everyone!
WHEW! You've made these past few days a fantastic journey! Here are two guys who share your passion:
Thanks to each and every one of you who... Read more
What's Next? Alan Shepard's Mercury Spacesuit
Alan Shepard's successful... Read more
Splash Down: We've Reached our $500,000 Goal!
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We've Hit Mission Midpoint! $250,000 Pledged on Day 2
More Limited Quantities Added
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Added 20 More for Online Q&A at $300
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Mission Status: $100,000
We're all doing the moonwalk because of you! We've soared to $100,000 in one day - and on the anniversary of Neil Armstrong's walk on the Moon. What a way... Read more
We're one day into our campaign and we've reached a major milestone - 1,000 Backers! Thanks for taking part in our mission to #RebootTheSuit!


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The Natural Way: Look younger while getting older
I saw a segment on the news about a clinic in Las Vegas that specializes in anti-aging. At $1,000 a week, you can get access to powerful prescribed drugs (including potential side effects) just so you can beat the reaper longer ... maybe. Anti-aging isn't the proper word. We all age; helping the body recuperate and fix itself is the key to looking younger as you get older.
Wrinkles — a sign of wisdom or not enough L-carnosine? The beneficial impact of this amino acid on skin and connective tissue are well-established. Skin (and connective tissue) benefits by having its life span increased. It can continue to divide and remain healthy longer. As we age, the connective tissue cells in the inner layer of skin dwindles because of the inability of the cells to divide. This leads to inelasticity, wrinkles and poor wound healing. Carnosine reverses this and according to Dr. William Campbell Douglas helps prevent aging throughout the body. Carnosine is a powerful antioxidant that the body digests best when taken with a quality probiotics and between meals. Additionally, Toki, a supplement of collagen blended with hyaluronic and dermatinic acid taken each day definitely replaces the padding under the skin, which puffs the wrinkles away and reduces dark spots. Though a little pricy, it is far cheaper than you might think.
A good antioxidant supplement will go a long way. Antioxidants do a lot of different things and are greatly beneficial. This is partly why a diet rich in fresh, colorful veggies is so good for us. An antioxidant supplement should include vitamins A, C, E (natural), B2, B6, zinc, selenium, copper, manganese, alpha lipoic acid, L-cysteine and L-glutathione (reduced). Some include CoQ10 but I like to supplement with larger amounts than typically found in most antioxidant formulas.
According to Dr. Ronald Lawrence of U.C.L.A., a surprise benefit of taking about 2000 mg/day of MSM is that folks may notice benefits such as softer skin, harder nails, thicker hair and softening of scar tissue. Hmmm ... better hair, nails, softer skin with fewer wrinkles — sounds good. A trip to Vegas can be fun but this can be done here in the Ozarks.


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The Darker Side of Hannah: Sublime Wolf 50:50 Merino Silk DK
0 in stock.
Regular price $35.00
The most popular in the #13reasonswhy colorway series
Sublime Wolf DK
50:50 SW Merino/Silk
242 yards
** Due to the fact that there exists a volatility when dyeing, no two skeins are going to be exactly alike, and as such, when using more than one skein in a project, it is advised to alternate between skeins to ensure consistency of colour.
Care: Hand wash, lay flat to dry.
** Not all wolves see the same blue when they look at the sky. A number of factors may be attributed to this phenomenon. Tech savvy wolves, might, on occasion, blame it on their computer monitors. **


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Finding and maintaining meaningful employment can have a big impact on promoting health and well-being, and helping people recover from mental illness. Our employment program helps job seekers access resources, skills, supports and connections with employers.
The Employment Program of BC, also referred to as EK Employment, is a network of four WorkBC Employment Services Centres. Through EK Employment Offices in Fernie, Cranbrook, Invermere and Golden, we provide information, services, programs and support to help individuals find and keep a job.
Job seekers can access self-serve services for their employment-related needs including:
- Job boards and job search resources
- Workstations, computers and internet access
- Printing, scanning and faxing services
- A resource library
All self-serve resource areas are hosted full-time by qualified staff who can set you up on a computer, proof-read your resume, or provide other basic advice to help you with your job search.
We can also help you access a number services including:
- Life and employment skills training
- Short-term certificate courses
- Employment coaching
- Career and skills assessment
- Work experience placements
- Volunteer placements
- Self-employment
Our offices are open Monday to Friday. For locations and more information, please visit our website below or call 1-855-651-3027 (toll-free).
Learn more:
- Read our 2017-2018 Annual Report – EPBC
- Visit us online at www.ekemployment.org
The Employment Program of BC is funded by the Government of Canada and the Province of British Columbia.


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Maine Gov. Paul LePage (R) assailed the Affordable Care Act in his weekly radio address today and declared the Internal Revenue Service, which will require Americans to purchase health insurance or pay an annual fee under the individual mandate, as the "new Gestapo."
"This decision has made America less free," Le Page said. "We the people have been told there is no choice. You must buy health insurance or pay the new Gestapo – the I.R.S.."
The Gestapo was Adolph Hitler's official secret police that terrorized Nazi Germany, imprisoning and murdering thousands of people.LePage took direct issue with the Supreme Court's ruling which upheld the law not under the Commerce Clause, but under Congress' taxing power.
"Perhaps what is most disturbing about this ruling, though, is that the federal mandate is considered a TAX," he stressed. "Now that Congress can use the taxation power of the federal government to compel behavior or lack thereof, what's next? More taxes if we don't drive Toyota Priuses or if we eat too much junk food or maybe even pea soup?"
Both Congressional Republicans and Mitt Romney argue, like LePage, that the Supreme Court's ruling makes clear the individual mandate is a tax. The Obama administration, however, maintains the mandate is a penalty collected through the tax code for noncompliance with the law. Indeed, that's what the government's lawyer argued before the Court.
LePage also joined many other high-profile conservative governors vowing to thwart the implementation of the law by indicating that Maine would not move forward with expanding Medicaid programs, an option made possible by the same Supreme Court ruling.
"We cannot afford our current programs, so to require Maine to expand coverage even more is fiscally irresponsible," he explained.
The governor further said the state would not begin setting up insurance exchanges under the law, citing "too many uncertainties" with federal matching funds, money that has yet to be approved by Congress.
Read LePage's full radio address here.


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Easier changing of intensity during ride
While pedalling away souring a workout, it is hard to adjust the intensity level. I don't stop pedalling, and it is hard to get finger onto the small button, and when you adjust you finger covers the intensity so you cannot see what you are setting to. Need easier way, more like the pre ride intensity setting


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You are using an outdated browser. Upgrade your browser today for a better experience of this site and many others.
01507 526071
- Boston: 01205 311966 - Spilsby: 01790 753340Bourne: 01778 393656 - Louth: 01507 609933 - Skegness: 01754 763222 - Lincoln: 01522 533588
Calculate the rate at which your savings will grow.
Enter the details of your savings and press the calculate.
The initial savings amount.
The monthly savings amount.
The average annual interest rate.
The average annual inflation rate.
The number of years to save over.
The amount to consider as the investment into the fund.
The expected annual rate of return of the investment.
Note: These calculations are only for illustrative purposes and are not a substitute for professional advice.
Keeping you up to date with the latest news from the world of accountancy


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- Home
- Solheim & Carradale Guest Houses
Solheim & Carradale Guest Houses
34-36 King Harald Street, Lerwick, Shetland ZE1 0EQ
Contact Name: Andrew or Caroline
Tel: +44 (0)1595 695275 / +44 (0)7748 774816
Email: email@example.com
Very friendly, comfortable guest houses, situated in the centre of Lerwick. A few minutes walk to shops, restaurants, Viking Bus Station, museum, cinema, leisure centre and across the road from Islesburgh Community Centre. There is ample free car parking in the immediate area. There are two beautiful coastal walks nearby and the family will be happy to help you plan your excursions or give suggestions to make the most of your visit to our unique islands...
- Solheim & Carradale Guest Houses
Establishment Overview
All rooms have TV, wifi, tea & coffee facilities and hairdryer. Guests may use laundry facilities free of charge. All rooms are en-suite except for our single standards with shared facilities at £35 pppn.
If you are visiting Shetland for a special event such as Up-Helly-Aa in January or the annual Folk Festival weekend in May, Lerwick is a prime location to be based at.
The guest houses are situated near to Lerwick's harbour, where boat trips are available throughout the summer to spot wildlife.
The guest houses offer single, twin, double and family rooms.
Free parking is available.
|Single room en-suite
|Rates start at:
|£45 per room per night
|Twin/Double room
|Rates start at:
|£60-£70 per room per night
|Family room
|Rates start at:
|£70-£90 per room per night


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|Palladium reaches all time high
|Wednesday, 20. February 2019 02:01
Palladium reached an all-time high on Tuesday and platinum prices also continued to soar, while gold and silver dropped slightly as investors waited for the next round of trade talks between the United States and China.
Palladium jumped 0.77% to sell for $1,491.88 per ounce at 1:55 am CET. Platinum climbed 0.13% to go for $821.52 per ounce at the same time. Gold dropped 0.04% to change hands for $1,340.17 per ounce at 1:57 am CET, while silver fell 0.12% to sell for $15.98 at the same time.
|Related Links:
Breaking the News / KP


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Seattle vs St. Louis Betting
Seahawks Look to Make it Three Straight – Hawks vs. Rams in Week 4
There will be a lot of talk about Monday Night's debacle heading into Seattle's Week 4 game against the St. Louis Rams. Truth be told, the Seahawks did enough in that game to win. A loss against the Packers would have been strangely acceptable but a win looks a whole lot better.
Seattle Seahawks (1-2) vs. St. Louis Rams (1-2)
Date/Time: Sunday, September 20, 2012, 1:00 PM ET
Venue: Edward Jones Dome
Broadcast: FOX
Hawks vs. Rams Betting Lines from betonline.com
Spread: Hawks -2 1/2 (-115)
Moneyline: Hawks -135: Rams +115
Over/Under: 38 points
ATS Matchup Stats | Gambling Insight! | Buy Ray's Picks!
Seattle heads out on the road this week looking for its third straight win – this week they face a team on the opposite end of the spectrum as their previous two combatants – the Rams have been underwhelming to say the least and occupy the bottom third of most expert's Power Rankings.
The Seattle Seahawks have proven that they are no joke. If they could have executed in the Red Zone in Week 1 they would be undefeated heading into Week 4. Rookie QB Russell Wilson hasn't been asked to do too much and the game plan is certainly working. He has turned the ball over just once in three games, he has tossed four TDs (well… three really if you count the last play in Week 3!!) and he has completed 57% of his passes.
GRAB OUR BEST Expert NFL Picks for 2012 FOOTBALL GAMBLING season! —–>
Marshawn Lynch has been the workhorse just as the coaching staff drew up in the preseason. Lynch is the emotional leader on the offense – he never gives up on a play and is a true difference maker for Seattle's young nucleus. When things are going well, Lynch should touch the ball 25 times – a look back at his performances the first three weeks has revealed just that!
While the offense has been efficient, it has been Seattle's defense that has truly raised a few eyebrows. They have become one of the best units in the NFL – 2nd against the run, tenth against the pass and allowing a league low 13 points per game. The Seattle defense is special and now has the honor of going up against a Rams offense that generated virtually nothing against the Bears last week.
The Rams are 1-2 on the season – he only win coming against the Redskins in a game that Sam Bradford had his best game in a long while and the Rams put up 31 points. Their other two games have been nothing to write home about – especially last week's woeful 23-6 loss at Chicago. They were inept on offense – just 160 yards in the game. Now they face an equally tough defense – Seattle which doesn't bode well.
The truth is that the Rams scare nobody on offense. Steven Jackson is already banged up and he went for just 29 yards last week. Sam Bradford is no Aaron Rodgers and should have a tough time with this defense. [soliloquy id="82219″]
The Rams on defense are nothing special but they likely won't have to be. They are 22nd against the pass and 21st against the run. The question is – can the offense do enough to keep them in this game.
Betting Prediction:
A few trends to consider:
- Seattle is 13-1 SU in its last 14 games when playing St. Louis
- Seattle is 6-1 SU in its last 7 games when playing on the road against St. Louis
- The total has gone UNDER in 4 of Seattle's last 5 games
- The total has gone UNDER in 4 of Seattle's last 6 games on the road
- The total has gone OVER in 4 of St. Louis's last 6 games at home
- The total has gone UNDER in 4 of St. Louis's last 6 games when playing Seattle
- St. Louis is 3-5-1 ATS in its last 9 games at home
- St. Louis is 5-14-1 ATS in its last 20 games
This game shouldn't be close. There will be a lot of talk about Monday night ahead of this game but the fact remains that the Hawks are sky high and as confident a team as there is in the game right now. St. Louis won't be able to score and likely won't be able to stop Marshawn Lynch. All together it looks like another long afternoon for Jeff Fisher and company.
Pick: Seattle Seahawks -2 1/2


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Items where Division is "Engineering & Applied Sciences > Engineering Education research group" and Year is 2017
|Up a level
Andrews, Jane E and Clark, Robin P (2017). Trailing or Failing? A Hidden Mental Health Issue: The Changing Futures Project. IN: American Society of Engineering Education 2017. America Society of Engineering Education.
Andrews, Jane and Clark, Robin (2017). Great expectations?:A comparative analysis of bachelor and graduate level engineering students' expectations of university and experiences of transition. IN: 2017 ASEE Annual Conference & Exposition. ASEE.
Andrews, Jane and Clark, Robin (2017). Work in progress: engineering invisible mountains!:mental health and undergraduate-level engineering education: the changing futures project. IN: 2017 ASEE Annual Conference & Exposition. ASEE.
Clark, Robin P and Andrews, Jane E (2017). From Trailing & Failing to Learning & Progressing:A bespoke approach to failure in engineering education. IN: REEN Annual Symposium. UNSPECIFIED.
Clark, Robin P and Andrews, Jane E (2017). Pedagogy, Practice and Procedure (The P 3 Project) - Educating Engineering Managers A Model for the Future. IN: 45TH SEFI ANNUAL CONFERENCE. Société Eropéenne pour la Formation des Ingénieurs.


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Fish Head - Fish Head Spin
The Fish Head Spin is perfect for those hard to catch suspended fish, as well as for locating fish that are feeding on bait fish as forage. Plus, it is great for schooling fish.The Fish Head Spin can be used with many different types of soft plastics.
Item ID: SH316FHS01
Color: Albino/Nickel
3/16 oz
1/4 oz
3/8 oz
1/2 oz
Quantity Available:
Item ID: SH316FHS03
Color: Citrus Shad/Gold
Quantity Available:
Item ID: SH316FHS04
Color: Green Albino/Nickel
Quantity Available:
Item ID: SH316FHS02
Color: Pearl White/Nickel
Item ID: SH14FHS110
Color: Smoking Shad
1/4 oz


text: Each Tuesday, the Bear in Boots Kitchen will create a different Tater Tot appetizer special highlighting seasonal ingredients or cuisine from different countries. One week, it could be Pizza Tots, the next week, Thai Tots. Each week will feature a beer and a dish inspired by each state in the USA. Each featured beer will be a limited release from a craft brewery and will be on the menu until it runs out. Once it does, we'll introduce a new $3 Craft Draft at the next Tap & Tot Tuesday! Our goal is to introduce you to as many new beers as possible, and to give you a reason to get out of the house on Tuesday nights.


text: Cantrell v Wright & Fuller Ltd This document is only available to subscribers. [2003] BLR 412 Construction contracts - certification - final certificate - definition/meaning of final certificate - interpretation of contract term as to final certificate - JCT Standard Form (1980 ed) The claimant employers contracted with the defendant under the JCT Standard Conditions of Contract (1980 edition).... This page is only available to isurv subscribers. Sign up for a free-trial to access this content. Alternatively, you can gain access now and purchase isurv online here.


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Following Tuesday night's disappointing 2-4 home reverse at the hands of Blyth Spartans, Trinity boss, Dave Frecklington has wasted little time in strengthening his squad that has become decimated by injuries since the big kick off on August 5th. This weeks' business sees the arrival of Lewis Collins, on loan from Mansfield Town.
At twenty years of age, Lewis is described as a reliable midfielder, with a range of passing that can unlock the most stubborn of defences. A Grimsby Town academy graduate, Collins impressed enough to be offered a deal at Championship club Reading. The midfielder then spent a six month loan spell in non-league at Farnborough FC, before signing a professional deal and joining Mansfield in the summer on the expiration of his contract with the Royals in the summer of 2016.
Lewis goes into the mix for Saturday's game with high flying Harrogate Town at the Martin & Co Arena. He is available for sponsor, by contacting Kev Housham for more details on
at only £50 + VAT. Home and Away shirts are available to sponsor. As is goalkeeper Louis Jones, who made his debut last weekend in the 0-1 away win against Nuneaton.
We welcome Lewis to the club. #WelcomeLewis #inittogether #Trinity #GainsboroughFamily
Specialists in Website Design, Content Management Systems and Online Marketing.
Gainsborough Trinity FC, The Gainsborough Martin & Co Arena, DN21 2QW. Get Directions.
Gainsborough Trinity Football Club are proud to be associated with Invest Gainsborough.
Web Design and SEO by Web Design Studios| Gainsborough Trinity FC © 2015|About|Contact
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5 tips to protect retirement investment
Retirement is the phase of our life where we can live a calm and peaceful life and enjoy the fruit of your labour. But it is observed that senior citizens struggle a lot to get a 2010 medicare advantage plan https://www.medicareadvantage2019.org/ lead a peaceful life worrying about their retirement investment along with the desire to invest their retirement fortune. In this article, I will suggest a few ways how to protect your retirement investment in this inconsistent market.
- Realising your Risk Tolerance
Risk tolerance is the amount of variation that one can afford to handle while investing in market. Risk tolerance is a very important factor since markets are prone to changes. So, identifying your risk tolerance will help you put forward a plan that will protect your investment.
- Create a Strategic plan and Follow it
Planning is one of the most important steps towards a perfect investment especially if you are a senior citizen above age 65. You should have a perfect plan on what you are going to invest, when you will be harvesting profits and come up with contingency plans for factors that might change. While devising the plan, always consider the past and present of what you are investing and factors like Risk appetite and tolerance.
- Manage a Diversified Investment Portfolio
Investment portfolio is the data or collection of your total assets and how you have organized them. Diversified Investment portfolio is the process of dividing your investments at different levels so that you can reduce the risk and expect an increased profit. A diversified Portfolio must contain a fixed income source to fund your retirement, and the rest is to be invested in a mixture of stocks and commodities. No matter however volatile the market turns out, some stocks will always go up while others are down.
- Hire a Portfolio Manager
Being a senior citizen and doing everything is hard work which you try to avoid in retirement. So it is advised to hire an expert portfolio manager for investing your assets and keep a diversified portfolio. While selecting a portfolio manager, we must be sure that he/she is an expert in this field. Developing your plan considering his/her advice can ensure your portfolio meeting minimum return requirements easily.
- Selecting stocks with Dividend
A dividend is an amount of money that an organisation pays regularly (usually annually) to the owners or shareholders from the annual profit that the company made in the financial year. Investing in stocks that provide dividends can ensure that your retirement is safe and following the above steps will help you protect your investments in these volatile markets.


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At the end of July 2013, I was enrolled at Indiana University Northwest and on what I thought was the final day of my internship at Aon Corporation. I was packing up to leave when the department vice president came by and told me I had been hired—full time.
Two years earlier, I was at Southern Illinois University (SIU) expecting to make the Dean's List, when my mother was diagnosed with lupus, an autoimmune disease that makes it difficult for her to take care of our family. I was already working to support myself and now I was also responsible for helping to support my younger brother and sister. That began to be more of a priority than my education. My lack of focus caused my grades to fall and I was academically dismissed from SIU.
I returned home and for a time, I felt helpless. My life had changed so much. Then my best friend DyKiesha, who was a Year Up graduate, told me about the program. She and I had similar career goals, so I thought that Year Up would be a great fit for me. Aside from learning more about professional skills, I learned a lot about myself at Year Up. I realized that I was more confused than I thought and needed to change. I had to practice being more assertive and find my life balance. But it was stressful having to learn a lot in so little time. From day one, a Year Up staff member connected with me and gave me the time management tools that helped me achieve a 3.83 GPA at Harold Washington College and receive the Presidential Scholar Award.
The most important thing I learned on my internship was to go into every situation with an open mind. I had expected Aon to be very conservative, but it was much more laid back, where everyone has a great sense of humor. My internship at Aon was in strategic sourcing, where I did data entry and ran monthly reports, helped plan meetings and events and worked on trade show logistics.
When I graduated from Year Up this year, I was proud that I had stuck with something and came out better than before. And, I was happy to see how proud my mother was. Now, I am working 35 hours a week at Aon and hoping to earn a degree in computer information systems with a minor in business at Indiana University Northwest by December 2015.
I used the opportunity Year Up gave me, turned it into full time job at Aon and have come to be a role model for others who need motivation to gain a better life. Urban young adults are figuring out how to positively help the communities and world we live in. All we need is the chance to learn, follow and lead. Invest in us because all of us are gold mines waiting to be discovered! We are the future!


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Game genres
Game settings
Random game
PC Games > Genre > Arcade > Happy Chef 2
Happy Chef 2
Big Fish Games
- OS: Windows XP/Windows Vista/Windows 7/Windows 8
- CPU: 1.4 GHz
- Memory: 512 Mb
- DirectX: 9.0
- Hard disk space: 349 Mb
Cook a variety of dishes from all around the world and become an expert chef in Happy Chef 2! Start from humble beginnings in an authentic Italian restaurant. Gain cooking expertise and travel to Hawaii, America, China, and France becoming a world famous chef. Upgrade your kitchen with fantastic new equipment, decorate your restaurant and serve a huge variety of customers with unique tastes and preferences in this Time Management game!
- Hundreds of dishes from around the globe
- Five unique locations to master
- Play Happy Chef
Other games
of this genre
|Big Pinata
|Alex Gordon
|Brave Piglet
|Beetle Bug 2
|Bud Redhead
Other games
of this setting
|All-Time Sudoku
|5 Realms of Cards
|Avatar Bobble Battles
|Arcadia REMIX
Other games by
this developer
|Enigma 7
|Cryptex of Time
|7 Wonders of the World
|7 Artifacts


text: Finius 575 Posted June 26, 2011 Take from the description on youtube: "A badman with seriously good looks makes a video diary about his relationship with his mum. comedy sketches about a troubled young man with the mentality of a 7 year old, growing up in the hood learning meaningful lessons along the way. " Basically takes the michael out of all these "gangsta" types down south, but done well. Awesome series. Here's a link to the first of many : NOTE: Could be interpreted as slightly racist, but since the guy is taking the piss out of himself, I don't think it counts... Share this post Link to post Share on other sites


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2019 Funded Study in UK: JRC Fuwaku Scholarship for UK/EU and Overseas Students
Need funding for master education? The University of London, SOAS and the Japan Research Centre (JRC) is offering the JRC Fuwaku Scholarship 2019 for eligible students in the field of Japanese Studies.
Application Deadline: May 1, 2019
Eligibility for the Scholarship:
- Eligible Countries: Applicants of UK/EU and Overseas are eligible to apply for the scholarships.
- Eligible Course or Subjects: Scholarships are available to study the Ph.D. or postgraduate Taught Masters Degree program in the field of Japanese Studies.
- Eligibility Criteria: To be eligible for the scholarships, the applicant must meet the following eligibility criteria:
- The scholarships are open for the UK/EU and Overseas applicants.
- The scholarships are available for MPhil/Ph.D. applicants, the research proposal must focus on Japan. The applicants should provide evidence of this in the research proposal of their admission application.
- The applicants who are enrolling in Postgraduate Taught Masters Degree program have must write their dissertation on a theme connected with Japan. Applicants should provide evidence of this in the statement of their admission application. For current Postgraduate Taught, Masters Degree should provide evidence of this in their Scholarship application.
- The Postgraduate Taught Masters Degree applicants must take a minimum of 1.0 units (or equivalent e.g 2x 0.5 units) on Japan/Japanese studies. Applicants (new and current) should provide evidence of this in their scholarship application.
- The applicants should include in the scholarship application details of their skills in Japanese. While the Japanese language is not essential, it is desirable so it will be helpful if the applicants' referees comment on their skills in Japanese in the areas of reading, composition, and speaking, if applicable.
- The candidates must have an offer of admission to a degree program at SOAS by the scholarship's deadline.
- The applicants must meet the English language condition of their offer of admission to study at SOAS University of London.
- The Applicants of current masters students enrolled in Year 1 in 2018-19 and entering Year 2 in 2019-20 are also eligible to apply.
Admission Requirements:
The applicants, who want to apply for the scholarship, must also submit an application for admission well in advance. Applicants can submit an online application for the admission through the given link:
- For the taught masters program: https://www.soas.ac.uk/admissions/pg/howtoapply/
- For MPhil/Ph.D. program: https://www.soas.ac.uk/admissions/pg/research/howtoapply/


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A HACKETSTOWN woman has won the coveted CEO of the Year accolade at the Image Business Women of the Year Awards.
Breda Quigley is the founder and managing director of Q Café Company, a contract catering firm that employs 180 staff and has wide variety of clients across the healthcare, business and industrial sectors.
The company, which was established in 1999, has previously won awards at the Hotel & Catering Gold Medal Awards and the Irish Foodservice Quality Awards, but this was the first time that Breda has won an individual award for her inspirational role in the company.
Her daughter and the company's business development manager Leah Moore, along with director of business Laura Olsen, submitted an entry for Breda without her knowledge.
"I was honoured to be shortlisted," said Breda. "We are very much a niche Irish business and we work very hard to build our business. We look after growers and suppliers as well. It's a great accolade for an indigenous business, as the judges look deeply at where you have brought the company from and what you have done to make it a success."
Breda was stunned to receive the gong at the prestigious awards in the Clayton Hotel last week. There were 74 tables at the awards and she wasn't optimistic when she was seated at table 74.
"I thought, oh well, that's okay, we'll have a good night anyway!" she joked.
Breda's childhood at the Curragh, Hacketstown greatly shaped her attitude and provides the core values behind the business. Daughter of the late Julie and Denis, Breda grew up in a family of seven.
"It was a warm, fun environment. There was an open-door policy with neighbours calling in for cups of tea."
Her mother was a great cook and Breda grew up with a love of food.
The Quigleys were hard workers and Breda said she never received anything but encouragement from her family to go and succeed for herself. She studied catering and worked in the sector for a variety of companies over the years.
In 1999, she became disillusioned with the business because of its focus on the bottom line.
"I wanted to look at something that I could enjoy," she said.
An unexpected opportunity then arose for Breda to go into business for herself, which she seized. Her love of family led her to name the business the Q Café Company.
For the last 20 years, Breda has been taking on the giants of the contract catering industry and succeeding. The business currently operates staff restaurants and cafés for 24 clients in Ireland, including semi-state, healthcare and blue chip companies.
It's a family business with Breda's husband Donal also working along with her daughter Leah.
The company has its own special approach, which is not solely motivated by profits and shareholders. It uses local food suppliers and producers at a client's site, while in recent years it has developed the Q Wellness brand for businesses that want to look after their employees.
"We have taken in some really key businesses based on our healthy approach to food and eating seasonally. We have tips on how people can exercise more. We have blended Q Wellness to deliver a bigger package," said Breda.
"Our whole basis is to grow carefully so we can protect our brands and we deliver on our promises. We are very committed. It's a family business. We are in business because of who we are and the style of catering we deliver. Every one of our people … we empower them to be creative. They love what they do," she added.
Breda has lived in Dublin since the early '70s but is still a proud Carlovian with a wide web of family in the Hacketstown, Tullow and Tinryland areas. Sadly, her eldest sibling Jim passed away earlier this month and Breda dedicated her award to him.
Reflecting on her time in business, Breda outlined the biggest lessons she had gleaned. "It's to continually learn and always keep an open mind about opportunities," she said. "Above all, believe in what you are doing and the ideas you want to deliver. If you don't believe that, you won't get your message through."


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2x40mm Colorful Pumpkin Porcelain Cabinet Ceramic Knob Drawer Pull Handle Kitchen Door Wardrobe Furniture Hardware
Item specificsType:Furniture Handle & Knob
Brand Name: China Arts ??
Model Number:8005-whiteColor:White
Material:Zinc Alloy&CeramicUsage:Drawer cabinetType:Modern delicate luxurious
Product Description
Quantity:2pcs as one set for saleColour: White
Material:Zinc Alloy&Ceramic Cystal Width:40mm Height:40mm Net Weight:68g Base Diameter:30mm Packing:individual pull with screw as one set to canton. This Price is wholesale price, we hope you can't miss this chance.
2 x Clear Knob
2pcs 40mm Colorful Pumpkin Furniture Cabinet Ceramic Knob Drawer Pull Handle Kitchen Door Wardrobe Hardware
Shipping DetailsItems will be shipped within 5 working days upon receipt of your payment. Items shipped from China using Airmail, reach most of the countries within 10 to 20 business days. Delivery time depends on destination, shipping method (economy or standard) and other factors, it may takes up to 20 business days. Item will be marked as a "Gift" and declared lower value for shipping purpose only. Buyers are responsible for any customs duties if applicable. Please make sure your address is correct. If you want to change your address, please send us ebay message or email as soon as you make the payment. Combing shipment is not allowed. If you want to combine all the items together, you should still pay for all of the shipping, insurance and handling fees.
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by Nigel Eatough
Date Added: 06/03/2017
05 March 2011 <br/>SIMPLY EXCELLENT Have ordered designed Polo shirts online and they have been poor in quality and service was poor. Thought i would risk Tshirt studio and i am glad i did. Design was easy. Shirt arrived very quickly and was as ordered and quality spot on. Communication and service was excellent. Would recommend to anyone!
Rating: [5 of 5 Stars]
by Julie
Date Added: 06/03/2017
Great ServiveLove my dog walking wellies, lots of good advice on the website and the order was processed and received in great time - got a txt telling me the hour time slot they would be delivered :)
Rating: [5 of 5 Stars]
by Paul YatesYork, GB
Date Added: 06/03/2017
Quality goods, quality service, quality pricesI have used Jules B several times now and found that there is a company that cares about the customer after the cash transaction. Jules B have a great selection of clothing, good prices and a service to the customer so far not seen or matched for a very l
Rating: [5 of 5 Stars]
by Hard Working Woman, Wales
Date Added: 06/03/2017
Efficient!!!Love the shoes - fit is great. Delivery exactly as advertised! Royal Mail (love them - they're local!) What I wanted (fitflop due black leather 8) was in stock, fits beautifully, and were on my feet exactly when I expected. What's not to love? Will be ordering a second pair from the same place, so I don't run them into the ground. Now, patent? Or leather again..... Hmmm..... Fitflops are so good for my feet!
Rating: [5 of 5 Stars]
by Jeremy Adsett
Date Added: 06/03/2017
Superb customer serviceI recently ordered some heel protectors from Supadance and made a mistake on my order, I contacted the custoner service team and they corrected my ordered in a speedy and efficient manner
Rating: [5 of 5 Stars]
by Andrea Strachan
Date Added: 06/03/2017
Brill!!!Was looking for a certain type of trainers for my sons Christmas, found them on Jules B Website, ordered them and had them within 2 days. The shoe box was also inside another box which i thought was a very clever idea as prying eyes would not know what it
Rating: [5 of 5 Stars]
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Keywords:Cabinet Knobs,door handles,Door Hardware,Cabinet Hardware,Decorative Hardware,kitchen cabinet pulls
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An ideal cutter for the cutest and elegant topper in the market. Just little effort to create this figure and decorate the cake in a very minimalistic fashion. Can be used to make cookies that can be decorated in no time because of the simplicity of the cutter.
Due to FDA regulations, no returns on products used to prepare or to manufacture edible things.
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A balanced, healthy diet should contain carbohydrates, fats, and protein. Fruits and vegetables, lean proteins, unsaturated fats and...READ MORE
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Akibento is a monthly anime subscription box that costs $29.95 per month. They promise $60 in value in each box, with a total of 6-8 items including an epic shirt. Items might include anime collectibles, figures, and more.
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Cooking Kingfish August 20, 2012 by Carolyn Shearlock Reader James Almond passed on this recipe for kingfish (also known as king mackerel) that does NOT require a smoker, yet still tastes great.
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31/07/2016 · Add a new layer and use the elliptical marquee tool to draw a semi circle. Invert the selection and fill with white. Reduce the opacity to around 29%. Invert the selection and fill with white. Reduce the opacity to around 29%.
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2 Bedroom Granny Flat Walking Distance to Roselands Centro Shopping Centre, Roselands Aquatic Centre and Public Transport More like house than a Granny Flat * Built in wardrobes to both bedrooms * Spacious open plan lounge area with Air Conditioning * Kitchen with gas cooking, stainless steel appliances and ample storage and bench space * Floorboards throughout * Internal Laundry * Spacious courtyard * Car space AVAILABLE NOW
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University of Hertfordshire
Title: MSc in Advanced Physiotherapy (Neuromusculoskeletal)
MACP Membership: Eligible for membership of the MACP upon successful completion of the MSc.
Course Tutor: Mary Sexton
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Tel: 01707 284689
Email: firstname.lastname@example.com
This well-established Master's degree is designed for physiotherapists who want to specialise and develop advanced skills and knowledge in neuromusculoskeletal physiotherapy. It includes a high proportion of relevant compulsory modules including a clinical practice element. The course is designed to enhance your current knowledge and understanding and give you opportunities to challenge and critically evaluate your professional practice. The aim is to advance your skills as a professional and develop your career so that you can practice safely, effectively and legally.
Professional Accreditation
On completion of the MSc Advanced Physiotherapy (NMS) programme you will be eligible for membership with the Musculoskeletal Association of Chartered Physiotherapists (MACP).
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The AT-SP series offers the latest industry standard in flexible, full-duplex Gigabit Ethernet connectivity.These hot-swappable, fibre interfaces simply plug into a SFP slot on Allied Telesis SFP compatible products. Configurations can be optimised to meet varied distance and service requirements. The AT-SP series offers short-haul and longhaul solutions. From the inexpensive AT-SPSX to the far-reaching AT-SPZX80, you have the option to extend your to the distances that meet your performance needs.The CWDM version allows you to take advantage the CWDM technology in an existing network infrastructure, maximizing fibre efficiency. Multirate versions allow technologies other than just Gigabit Ethernet to be transmitted over single mode fibre. The AT-SP series SFPs are compatible with all the Allied Telesis range of products support SFP uplink connections.
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There may be nothing more thrilling in a person's life like shopping for a brand new residence. Everybody is filled with decorating concepts for turning the brand new home into a home. However, before splurging on a fancy sofa or cutting-edge kitchen, you should guarantee that your new place is suitable to stay in.
In any case these things have been taken care of, it's time to even off the partitions and add some coloration to the rooms. The choice of colours needs to be made both according to the model of the whole residence or according to the model of every room. However, just remember to take note of what kinds of curtains, carpets and furniture you intend on utilizing in every room. The big selection of merchandise accessible on the market ought to make it simple so that you can select one thing appropriate in your taste. Whether you go for some neutral tones like beige and cream, or for daring shades of crimson and yellow-green, guarantee that the color you select won't be too tiring and that it's going to make one of the best of the room you selected it for.
This looks as if probably the most pleasant part of the redecorating course of. However, some individuals are very reluctant to deal with painting because of the very unpleasant smell that is involved. Weeks after the painting job has been finished, the chemical odor still persists in the room.
Thanks for visiting my blog, article above (Pleasant Nightstand Set Of 2 High Resolution Pertaining To Our Household) published by Trevor Mills at October, 19 2018.
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Assange lawyer says espionage charges against WikiLeaks founder are 'threat' to all journalists Washington Post
Assange lawyer says espionage charges against WikiLeaks founder are 'threat' to all journalists.
US files new charges against Wikileaks founder Assange CNN
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The U.S. Justice Department unveiled 17 new criminal charges against WikiLeaks founder Julian Assange on Thursday, saying he unlawfully published the ...
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WikiLeaks founder Julian Assange is facing a new round of charges for his role in publishing tens of thousands of US government documents provided by former ...
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Edward Snowden said the Justice Department declared "war" on journalism after accusing Wikileaks founder Julian Assange of violating the Espionage Act.