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A Ford man joins the "family" philanthropy
A Ford man joins the "family" philanthropy
Henry Ford III is joining the "family" philanthropy. The scion of the famous family is the newest board member at The Ford Foundation – and the first Ford to serve as a trustee since his grandfather, Henry Ford II. Current foundation President Darren Walker played a key role in bringing in Henry Ford III for a six-year term, according to Feb. 25 press release.
"Ever since Darren became president, he's done a really good job reaching out to the family," Ford said in the press release. "He's given us updates and kept us involved in the foundation."
A spokesman clarified post-publication that the philanthropy – which was established in 1936 by Edsel Ford and Henry Ford – is an independent foundation.
Rising Ground has received a special proclamation from New York City Mayor Bill de Blasio. Cecile Noel, commissioner of the Mayor's Office to End Domestic Violence and Gender-Based Violence, was on hand to present the honor on Feb. 25, which recognizes the nonprofit's STEPS to End Family Violence program.
Commissioner Noel presents an awesome proclamation to the @risinggroundny @STEPStoEndFV #RAPP program! pic.twitter.com/pFnGyiU8Uo— Rising Ground (@risinggroundny) February 25, 2019
The Institute for Family Health has received a $100,000 contract from the New York City Department of Health and Mental Hygiene. The money will fund affordable health care for immigrants, according to the City Record. Planned Parenthood of NYC has received a $303,125 contract from the agency to provide reproductive health services.
RiseBoro Community Partnership and St. Nicks Alliance are behind a new 20-unit affordable housing development in Brooklyn. The New York City Planning Commission gave the green light on Feb. 13 for the project to continue, according to the New York Law School CityLand blog. The project, located on city-owned land at 63 Stockholm Street in Bushwick, will be 100 percent affordable when it opens. Read more here.
The NoVo Foundation has announced $10 million in new funding for reducing commercial sex trafficking. The three-year commitment will fund the Life Story Grants initiative, which focuses on people affected by the sex trade, according to a Feb. 20 press release. The New York City-based nonprofit is currently accepting letters of inquiry.
"System failures call for systems-based solutions to create lasting change – and that's where we see an untapped opportunity for anyone who wants to improve the lives of marginalized girls and women," Pamela Shifman, executive director of the NoVo Foundation, said in the press release. "Practitioners in critical systems – like teachers, social workers, bus drivers, police officers, emergency room doctors, and immigration officials – come into contact with people in sexual exploitation every day. By offering compassion, resources, and opportunity, these practitioners can close an on-ramp to exploitation – or open an exit ramp."


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My name is Brooke, I'm a Canadian Mom Blogger who trys to answer the million and one questions new moms have, and tell my own stories along the way. I would love to live in a world where every new mom knows exactly what to do and when. But since that seems unrealistic, we need Mom Blogger's like me. I've been working hard to make sure every mom has the information they need to make it through the Newborn phase and beyond.
When I'm not blogging, you can find me hanging out with my own baby, Hadley, who is still under a year. I hope every new mom out there knows that they are not alone, and Mother Me Crazy gives you a sense of community.
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Sarasota Memorial's medical staff recently honored two physicians for outstanding service to the hospital and the community: Longtime Sarasota cardiovascular surgeon Thomas Kelly, MD, was awarded the health system's 2015 Lifetime Achievement Award, while neurologist Mauricio Concha, MD, was named the 2015 Physician of the Year.
Dr. Kelly was honored for his pioneering role in the health system's cardiac surgery program – he helped found the program in 1983 – and his ongoing leadership as a hospital board member and medical innovator. Dr. Concha, who came to SMH in 2001, was recognized for his influential work as medical director of the hospital's stroke program. He spearheaded a number of advances in stroke care that resulted in Sarasota Memorial's designation as a Comprehensive Stroke Center and clinical outcomes that are among the best in the nation.


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How to make 2 objects 1 in illustrator - Good way to create an array in Adobe Illustrator CC 2014 .1.1
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Home > Expired Vacancy
Are you looking for a new Biomedical Scientist role in Cornwall?
Sanctuary Health is currently looking for an experienced and dedicated Band 6 Biomedical Scientist to work in a Clinical Chemistry department in Cornwall. The ideal candidate will possess highly developed specialist knowledge of Biomedical Scientist practices and be registered with the HCPC.
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The Danish film The Guilty will be released in the U.S. later this month, and to build up the release the film magazine Empire has released an exclusive clip from the film.
Directed by Gustav Moller, The Guilty follows alarm dispatcher and former police officer Asger Holm (played by Jacob Cedergren, Submarino). He answers a call from a woman who claims to be kidnapped, and when the call disconnects he desperately searches for the woman and the kidnapper, with only his phone as a tool for locating them. The whole film is shot within the emergency call centre.
A U.S. poster has also been released for the film, but boy is it bad. It's so over-the-top for the film, which is actually very stripped back and minimal.
The Guilty is Denmark's selection for the Academy Awards this year. It will be released in U.S. cinemas on the 26th of October 2018.
Watch the clip below.


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However, all he ever wanted to do was protect Alice, who loved him when he was only a stuffed rabbit doll and gave him a name and place to belong. Sully from Monsters, Inc. His appearances in the first season consisted of the intro, the outro and a cameo that barely lasted a second.
He might even be an actual monster. This can let students see what the director felt was best. He can heal other beings and even bring them back from the brink of death, but it takes a heavy toll on his body. And that is a great thing for everyone. The scene ends at 6: Saul, a giant Nico Robin met in her childhood.
The Magic High Commission seem to seriously think "monsters" inherently untrustworthy, to the point of replacing Mewni's legitimate heir with an impostor just to keep one from gaining magic power.
They're trying their best to find the leak in magic while still doing incredibly important jobs to the function of the multiverse.
Healing Magic Is the Hardest: The size of the fireballs are larger if Bowser is given a Super Mushroom. Lannes, displaying such huge balls that their gravity started attracting debutantes, snatched the match from his hand and angrily insisted that since a truce had been signed, the sergeant was destroying public property, and if he tried it again, Lannes would have him arrested, goddammit.
Continue Reading Below Advertisement Oh, and as if his fate were being written by the vengeful spirit of a vaudeville comedian, Hughes discovered that his targets were a little over a half mile away, which, powerful wind notwithstanding, was beyond the range of the rifle he was using.
During the scene, he stops his memories of what Bubblegum told him about the plan by having her explode when causes different sizes of herself talking about the plan. Stumbo the Giant from Harvey Comics His first name is similar to the Omnitrix and his name in general seems to be based off Optimus Prime Size Shifter: A harsher version is Gin Ishida from the Shitenhouji team.
Once you get to know him, that is. When not in the basket courts, that is. He's also a fairly mellow guy who is perfectly willing to make himself a decoy to ensure the escape of those under his command. Chien-Po from Mulan is soft-spoken and loves to eat more than anything, but his Stout Strength is such that he can lift the weight of several fellow soldiers and a horse, all at once.
And they're made out of swords, of all things. Shuh and Toki also follow this trope to a less obvious degree, although not quite as tall. Has to sit on the floor a lot because he's just too tall to function in most rooms.
When the battle begins, Bowser is fought in a similar manner as in Super Mario Bros. Kazuichi Banjou from Tokyo Ghoul. After placing Star in his guts, located in his torso, by hand he steps in after her. They come across a castle which is owned by an evil wizard who uses them as his test subjects for a shrinking potion.
Brent Staples finds many ways for us to catch his message using rhetorical devices.Thank you! Comments like your motivate to make more instructional materials like this list.
I created the list for a Hotel and Restaurant ESL class but knowing it would ultimately be shared with a wider audience on the internet caused me to put the extra effort into the project.
Rugrats is an American animated television series created by Arlene Klasky, Gábor Csupó and Paul agronumericus.com show focuses on a group of toddlers, most prominently Tommy, Chuckie, twins Phil and Lil, and Angelica, and their day-to-day lives, usually involving common life experiences that become adventures in the babies' imaginations.
Adults in the series are almost always unaware of what the. Name Vote; Guitar Hero III Custom: HARDcore: Vote: New Super Mario Bros. Wii ANDY AFRO'S Custom Collection Volume 2. Vote: New Super Mario Bros.
© Designed for agronumericus.com - Watch Anime Online. The Gojulas Giga (RZ) is a Giganatosaurus-type Zoid, one of over species of biomechanical life forms around which the Zoids model, anime, and game franchise is based. The Gojulas Giga is a major Zoid in the Zoids: Fuzors anime.
Ano Natsu de Matteru:Bokutachi wa Koukou Saigo no Natsu o Sugoshi nagara, Ano Natsu o Matteru.Download


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The Nissan GT Academy Philippines officially concluded its first live recruitment event in the global search for the next gamer to race driver at the Glorietta 5 Activity Center in Makati City. Two-time AutoIndustriya.com Gran Turismo 5 Challenge Champion Terence Lallave topped the first weekend of the Nissan GT Academy qualifiers with a 1:03.096 best laptime.
Twenty-nine-year-old Lallave is an avid car enthusiast and gamer has been a fan of the Gran Turismo franchise. He has long been wanting to qualify for the program since it was launched in 2008. Unfortunately, the Playstation Network does not support the Philippines, so qualifying online from the Philippines was not possible.
"I hope to make it to the national finals and eventually the elusive Top 6 who will represent the country in Silverstone. I am really thankful that Nissan Philippines has brought what used to be a dream to our country. It has long been a dream of mine to become a real race car driver," said Lallave.
Nissan GT Academy is a partnership by the automaker's motorsport arm Nismo along with Sony's Gran Turismo to develop professional race drivers from the racing simulation title. It has since fielded "Nismo athletes" to events like the 24 Hours of Le Mans, Dubai 24 Hours, Nurburgring 24 Hours, Bathurst 24 Hours and other prestigious events.
Nissan Philippines officially launched the local GT Academy program in late March with live recruitment events from April until June 2015. The Top 20 qualifiers at the end of the recruitment period will advance to the National Finals. The 6 best drivers in the National Finals will represent the Philippines in the international event at the Silverstone Circuit in the UK.


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Project Partners is a Proud Sponsor of OAUG Collaborate 19
Our expert team will be on-site at COLLABORATE 19 from April 7-11, 2019, Henry B. Gonzãlez Convention Center, San Antonio, TX
Bringing together attendees from three independent Oracle Applications users groups; IOUG, OAUG and Quest.
There is still time! Click here to register for the conference.
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We look forward to hearing more about your needs and how you can elevate your organization with Project Partners' proven innovative Oracle ERP Cloud, EBS, and Primavera solutions for your industry.
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Session #10708: Two Birds with One Stone! Automate Resource Planning with Project Financial Planning
Presented By: Neeraj Garg, VP of ERP Solutions
Non-traditional PSOs like Engineering firms do not employ "Staffing Managers" for resource allocation. This burden falls to project and resource managers. Resource Management applications require these managers to assume the burden of maintaining staffing data in addition to their regular duties. Neeraj will show how financial planning in Oracle Projects can automate the staffing function. PMs plan and execute their projects using budgets and forecasts while RMs get a view of where their resources are deployed, and Operations Manager get Resource Supply & Demand reporting for strategic planning.
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Improve Project Planning and Control with Oracle Cloud PPM
Planning and controlling projects is critical to delivering successful and profitable projects. Learn how to leverage Financial Project Plans to plan your project and budget in conjunction with automatically generated periodic forecasts to monitor and control your project spend when using Oracle Project Portfolio Management Cloud.
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Cloud Process Scheduler (CPS) for Oracle Cloud Applications enables Operations and Finance Managers to utilize Microsoft Excel as a user-friendly interface for running all scheduled processes for Oracle Cloud transactions with a single click. This topic/demo will show you how you can configure and use the CPS to define scheduled processes, set fixed and variable parameters, setup process dependencies, kick-off an entire set of processes with one click, and monitor the status of each process in the set as it completes.
Cloud ERP Reporting for Services Organizations Using Project Partners' Services Resource Planning™
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Shawholm Crescent, Glasgow, G43.
Trust Housing Association Ltd is a housing association providing a range of low cost social housing to people needing needing somewhere to live. Based in Shawholm Crescent, Glasgow, Trust Housing Association is a housing association.
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One stroke
The more this Wang Kui is like this, Jiang Chen's heart is more funny. Undoubtedly, the more this Wang Kui is, the more Jiang Chen suspects that this person is outgoing fierce but inwardly faint-hearted.
Really strong and powerful, it is completely unnecessary to intimidates opponents in this way.
The momentum of the strong, displayed in the ring, is called the strong.
In the downfall, Yaowu is just a small road. Not enough to scare people. Who can get to the final 64-person list, who will be scared by the eyes?
Jiang Chen will not, other people will not estimate.
Jiang Chen ignores the innocent performance. In Wang Kui's view, it is a kind of timid performance. This makes Wang Kui more proud, and he thinks that Jiang Chen has no courage to look at him.
Jiang Chen ignored this Wang Kui, innermost feelings calm, admiring the battle in the ring. This is the duel of the Great Emperor Realm, but the scene of this matchup is wonderful.
Undoubtedly, the Extended Emperor Realm genius of Extended Winter Island, on the cultivation base, on the combat battle efficiency, on the understanding of Martial Dao, is indeed ahead of a plan by Human Territory's Great Emperor Realm.
Jiang Chen feels that this place of Extended Winter Island, although desperate, to a certain extent, the potential of martial artist is also intimate stimulation stimulation.
Who is willing to be lazy in this kind of poor living conditions? Who can be lazy?
Naturally, Great Emperor Realm's duel, after all, is the duel of the Great Emperor Realm. In the case of limited aspects, the pure Great Emperor Realm showdown, this level of combat, for Jiang Chen, is not a big big challenge.
After all, when Jiang Chen was at the Emperor Realm early rank, had already played against Great Emperor Realm and was still in confrontation with top rank Great Emperor Realm.
Now, Jiang Chen himself has stepped into the Great Emperor Realm, and the strong Emperor Realm level of Human Territory, Jiang Chen confidently can sweep.
This Great Emperor Realm showdown in the ring, although Jiang Chen is somewhat amazing. However, Jiang Chen present's altitude, obviously confident, feels that the realm of own is already above the same level cultivator.
The vision presented should be looking to the strong Celestial.
The strong Emperor Realm of the same step, Jiang Chen does not feel threatened to himself. Even if you don't use the heaven defying stage prop, even if you don't call the contract beast, Jiang Chen can confidently handle it.
In a battle, the tactical situation is very intense. One-sided battles rarely occur. Basically, every battle will go into a state of heat.
It can be seen that the Great Emperor Realm genius entered the final 64-person list, and the disparity between the strengths of each other is not particularly large.
Naturally, there are occasional overwhelming advantages. However, this kind of scene is rare.
Every time you appear, the winner will be considered a big popular for the final eight.
Finally, it was Jiang Chen's turn to take the stage.
Wang Kui couldn't wait any longer, stepping into the meteor stride, rushing to the top, invincible might, and looking down at Jiang Chen from the ring.
Jiang Chen laughed dumbly, facial expression was light, and slowly walked up.
On the stage, Jiang Chen looked at him and looked at Wang Kui's self-confident look. said:
During the speech, Jiang Chen's pupil suddenly shot a golden light.
Evil Golden Eye is like an evil spirit lightning.
This Evil Golden Eye is under the constant cultivation of Jiang Chen, and today's power has reached a very fearful situation. With the same order of opponent, almost no one can hold the strong great power of this Evil Golden Eye.
Wang Kui was overconfident and didn't pay attention to Jiang Chen. It was just a potential preparation that launched a fatal blow to Jiang Chen.
Evil Golden Eye shot a golden light, Wang Kui whole body quickly grasps the meaning of something.
Next one quarter, his fierce's eyes suddenly sluggish, next quarter, his state of mind seems to be detained by some kind of power, suddenly became dizzy.
This is in the ring, although the rules allow killing, but Jiang Chen does not want to leave a impression of cruelty.
Therefore, Jiang Chen Sky Leviathan Escape Stream suddenly spurred, the body turned into a lightning, like an electric Roc, wings fluttering, has already rushed to Wang Kui.
A handprint, a virtual giant has been sent to Wang Kui.
Wang Kui is like a fixed movement skill. Pushed by this superhuman hand, the body is like a broken kite, desperately falling down the stage.
There was a splash of dust on the platform, and Wang Kui's burly body had already had an intimate contact with the ground, in an extremely difficult situation.
This fall, Jiang Chen's eye technique is also just closed. Wang Kui's mind regained his freedom, the whole body glimpsed, and he recovered Divine Consciousness, only to find that he had been stunned in the downfall, and the whole body fell sevenseight, how much more wolf.
This time, Wang Kui, there are still some gods. He obviously hasn't slowed up, how can breathe, and he's just a moment of sorrow, and he's lost? defeated so unfathomable mystery? Lost so much?
Wang Kui angered from the heart, an unprecedented shameful feeling, let Wang Kui scream:
Wang Kui couldn't find any other reasonable explanation. Only the demon method can explain everything that faces.
Wang Kui was annoyed and shouted:
Wang Kui is big, but the brain capacity is really good. This is obviously a question of the impartiality of the referee.
The referee of this game is really complexion's sinks:
Referee's words, such as the same pot of ice water, have ruined all of Wang Kui's self-confidence.
In the secret room, the City Lord's Mansion, who watched the battle, was amazed.
These staff members are concerned about the performance of Jiang Chen, and they are also extremely interested.
Some people think that Jiang Chen has such performance as having fortuitousness. But more people have realized that this youngster, indeed, as City Lord Sir judged, has great potential.
The next matchup, the number of splendid is not missing.
Finally, the first round thirty-two field showdowns, all completed. The failure of each matchup is equal to the loss of the impact of the quarterfinals.
The 32 winners will enter the second round of the duel.
This second round will determine 16 winners. The 16 winners will play the third round of the match and decide the final eight.
There is no doubt that now the distance from the final eight is only two steps.
These two steps, also let the batch genius who marched into the thirty-two strong, one by one dignified, fighting intent. Whose heart is clear.
Stepping out of these last two steps, they will become the offerings of the City Lord's Mansion and will be fully integrated into the high-level team of the City Lord's Mansion.
For them, it is absolutely unprecedent's opportunity!
In a matchup, there are only two double-hour breaks, and there is no doubt that time is very tight.
Especially the people who won the first hard battle are even more bitter. After all, after hard work, strength and energy did not recover so quickly.
The time for two double-hours is not enough.
Fortunately, these people are top rank genius, most of them have medicinal pill of different types. In this urgent need to restore strength, they also had to take this big medicinal pill out to swallow, in order to restore strength as quickly as possible.
The first round struggle, the second round is a harder fight.
Therefore, these geniuses will not regret it.
In the first round of fighting, Jiang Chen was a few of the more relaxed. It can be said that he did not start warming up with a warm-up, and a look has already solved the battle.
Therefore, this round fight, there is no consumption at all.
Two double-hour, Jiang Chen is not wasted, eyes closed meditation, the scene of every battle he sees, one by one has been back and forth in his mind several times.
Although Jiang Chen is very confident, but he and other side, he still understands.
Through this battle, he can also find out from his he winners where the advantages of these people are.
So come here. Jiang Chen heart in the docks of these opponents, also have a general strength understanding.
Two double-hour hours, so quietly passed.
Jiang Chen's current number is No. 9, a more vocal number.
The atomic in the pumping is the 17th.
Jiang Chen remembers that this 17th seems to be a young fatty. This young fatty, the combat aspect is more slippery, although the first knockout win, but not the overwhelming advantage.
Sure enough, there was a little fat fatty over there, not far away, with a more complicated look, secretly looking at Jiang Chen.
There is no doubt that this fatty is different from Wang Kui. The fatty heart is very bright. It is also because he is a smart person, so after seeing himself select Jiang Chen, my heart is obviously somewhat lost.
Obviously, Jiang Chen is not the opponent that he wants to encounter!


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April is National Grilled Cheese Month and in celebration I have a guest post over at Wisconsin Cheese Talk. Who doesn't love a delicious grilled cheese sandwich? Mine is filled with crimini mushrooms (aka baby portabellas), creamy Wisconsin Havarti cheese and fresh basil. I've got you drooling already, haven't I? Jump on over and check out the recipe and Happy Grilled Cheese Month!
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A silver‐stained protein gel showing the TBP‐binding assay of wild‐type and Spt3‐401 (E240K) SAGA complexes. 3 μg SAGA was immobilized to calmodulin beads and incubated with 0.5 μg recombinant TBP (rTBP). EGTA eluted samples were analyzed for TBP binding. Stars denote bound TBP.
Western blot analysis of the above TBP‐binding assay probing for Ada1 and TBP.
Source data are available online for this figure.
Source Data for Figure 1A, B [embj201488638-SourceData-Fig1A-B.pdf]
SAGA subunits are color‐coded according to the different modules they belong to. Domains are highlighted as indicated based on previous studies or HHpred predictions (with > 95% probability score). Grey and red dots represent lysine residues (not crosslinked and crosslinked, respectively), while grey lines connecting red dots denote intermolecular crosslinked lysine pairs. SEP: Shp1, Eyc and p47; SCA7: spinocerebellar ataxia type 7; ZZ: zinc‐binding domain; SANT: SWI3, ADA2, N‐CoR and TFIIIB" DNA‐binding domain; SWIRM: SWI3, RSC8 and MOIRA; Taf5 NTD: Taf5 N‐terminal domain; FAT: FRAP, ATM and TRRAP; FRB: FKBP12 rapamycin binding; FATC: FAT C‐terminal.
A–D Crosslinking network for (A) TAFs/Ada1, (B) Spt7, (C) Spt20, (D) HAT, and DUB modules. SAGA subunits or modules are represented as spheres and are color‐coded similar to Fig 2. Grey lines connect crosslinked SAGA components, with the width of the lines proportional to the number of intermolecular crosslinks. Transparent spheres in (B), (C), and (D) denote the subunits or modules that do not crosslink to the highlighted components.
A mini‐crosslinking map between TBP and SAGA subunits.
Surface representation of TBP (PDB ID 1TBP). TBP residues that crosslink to Spt8 and Spt3 are colored magenta and blue, respectively, with BS3 crosslinked residues underlined and BPA crosslinked residues boxed. Residues crosslinked to Spt7 (7) and Spt20 (20) are colored green. A conserved positively charged groove on the TBP surface is colored yellow. A hydrophobic pocket on TBP is shown as a red circle. Residues on the TBP surface that have been shown to suppress Spt3 mutations are colored white (Eisenmann et al, 1992; Laprade et al, 2007).
Representative Western blot and Coomassie‐stained gel of 6His‐TBP pull‐down experiments are shown on the left. 10% eluates were loaded each lane. Relative band intensity for GST or GST‐Spt8 is shown as bar graph on the right. Intensity of GST‐Spt8 bound with WT TBP (lane 6 on the left) was set to 1. Error bars represent standard deviation from three independent experiments.
Source Data for Figure 4C [embj201488638-SourceData-Fig4C.pdf]
A–C Deletion schematics for (A) Sgf73, (B) Ada2, and (C) Ada3. Numbers indicate deleted residues. Sgf73 is colored purple, and Ada2 and Ada3 are colored green. Colored circles above wild‐type (WT) constructs denote the crosslinking partners to corresponding regions: orange, Spt7/Spt20; purple, Sgf73; dark green, Ada2/Ada3; blue, Tafs/Ada1. Sgf73 and Ada3 constructs have a 3× Flag epitope tag at the C‐terminus; Ada2 constructs have a 3× Flag epitope tag at the N‐terminus. Boxes summarize the IP experiments in the lower panel: black, required for the association of the DUB module; red, required for the integrity of the HAT module; dark green, required for the association of the HAT module.
D–F IP experiments for (D) Sgf73 mutants, (E) Ada2 mutants and (F) Ada3 mutants are shown. Upper panels: anti‐Flag IP; lower panels: anti‐Spt7‐TAP IP. Proteins are visualized in Western blot (WB) with corresponding antibodies labeled on the right of the blots.
Source Data for Figure 5D, E, F [embj201488638-SourceData-Fig5D-E-F.pdf]
A silver‐stained protein gel showing the purification of Sgf73 Δ4, wild‐type (WT), Sgf73 Δ2A, and Ada3 Δ2A SAGA complexes. DUB subunits are colored purple; HAT subunits are colored green; Sgf73 Δ4 complex is colored pink; WT complex is colored blue; Sgf73 Δ2A complex is colored orange; Ada3 Δ2A complex is colored light green.
iBAQ analysis of WT, Sgf73 Δ2A, Ada3 Δ2A, and Sgf73 Δ4 SAGA complexes. Only iBAQ values normalized to Spt7 for DUB and HAT subunits are shown. See Supplementary Fig S5A and Supplementary Table S4A for iBAQ values for other SAGA subunits. Error bars represent standard deviation from 4 replicates for WT complex, 2 replicates for all three mutant complexes. Color scheme is the same as in (A).
WT and Sgf73 Δ2A SAGA complexes were analyzed in an in vitro HAT assay using mono‐nucleosome as substrate. Equivalent amounts of Gcn5 and all 4 histones were present at each time point as visualized by Western blot and silver staining (upper panel). Reactions were monitored by Western blot using antibody against acetylated H3K9 (Acetyl‐H3). The acetylation signal was normalized to the amount of Gcn5 in the reaction at each time point (lower panel). Error bars represent standard deviation from 4 experiments (two biological replicates with two technical repeats). Color scheme is the same as in (A).
Same as in (C), but comparing HAT activity between WT and Sgf73 Δ4 SAGA complexes. Sample from Sgf73 Δ2A complex at 30 min time point was also loaded as a control. Error bars represent standard deviation from 2 biological replicates.
Source Data for Figure 6A, C, D [embj201488638-SourceData-Fig6A-C-D.pdf]
SAGA subunits were positioned around the TFIID core EM model (see Discussion section for details). Homology models of yeast Taf proteins and Ada1 were superimposed onto the coordinates kindly provided by Patrick Schultz (Bieniossek et al, 2013) and color‐coded as indicated. Spt7/Taf10 HF (Histone Fold), Spt3, Spt8 WD40 are homology models (see Materials and Methods for details). Crystal structures used: TBP, 1TBP; DUB, 3M99; Taf5 NTD (N‐terminal domain), 2J49. HF, histone fold domain; Spt7N/C, Spt7 N‐terminal/C‐terminal region; Sgf73C, Sgf73 C‐terminal region. Images were prepared using UCSF Chimera (Pettersen et al, 2004).
Supplementary Figure S1 [embj201488638-sup-0001-FigS1.pdf]
Supplementary Figure S2 [embj201488638-sup-0002-FigS2.pdf]
Supplementary Figure S3 [embj201488638-sup-0003-FigS3.pdf]
Supplementary Figure S4 [embj201488638-sup-0004-FigS4.pdf]
Supplementary Figure S5 [embj201488638-sup-0005-FigS5.pdf]
Supplementary Figure S6 [embj201488638-sup-0006-FigS6.pdf]
Supplementary Table S1 [embj201488638-sup-0007-TableS1.pdf]
Supplementary Table S2 [embj201488638-sup-0008-TableS2.xlsx]
Supplementary Table S3 [embj201488638-sup-0009-TableS3.pdf]
Supplementary Table S4 [embj201488638-sup-0010-TableS4.pdf]
Supplementary Table S5 [embj201488638-sup-0011-TableS5.pdf]
Supplementary Movie S1 [embj201488638-sup-0012-MovieS1.mp4]
Supplementary Methods [embj201488638-sup-0013-Methods.pdf]
Supplementary Legends [embj201488638-sup-0014-Legends.pdf]
Source Data for Supplementary Figure S1B [embj201488638-sup-0015-SourceData-FigS1B.pdf]
Source Data for Supplementary Figure S5B [embj201488638-sup-0016-SourceData-FigS5B.pdf]
Source Data for Supplementary Figure S5C [embj201488638-sup-0017-SourceData-FigS5C.pdf]
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The Influence of the Quantum Tunneling Phenomenon on Enzymatic Kinetics
Albert Jin and Brandon Walther
It is known within the scientific community that quantum tunneling plays a role in electron and proton transfer during enzymatic catalysis1-4, and certain mechanisms have been devised to describe these systems5,6.
The origins of this assumption arose from apparent disparities between measured and calculated rate constants within certain proteins that employ electron or proton transfer. Stemming from this correlation between electronic/hydrogenic transfer and increased rate constants, quantum tunneling was introduced into protein dynamics and catalysis to attempt to explain this phenomena. Experimental testing of the kinetic isotope effect (commonly used in chemical kinetics as well7,8) further solidified this hypothesis and tunneling was then considered an important factor in enzymatic catalysis.
This project seeks to develop a model that will mathematically mirror the tunneling phenomena's influence on the rate constants evident in nature.
The system was simplified to a one-dimensional, double-well harmonic oscillator which can be solved exactly to yield a transmission coefficient. This transmission coefficient, which relates the quantum rate constant to the classical rate constant, was then graphed to provide its dependence on the activation energy of the system (this value is analogous to to the height of the potential barrier in the wavefunction model of the system). It was found that many variable contribute to the degree of tunneling evident in protein catalysis such as temperature, internuclear distance, and, as stated earlier, the potential barrier.
In order to ascertain the accuracy of the model, the values that would be theoretically predicted using the model were applied to the kinetic isotope effect, which is a ratio of the rate of the light isotope to the heavy isotope that is often cited in literature7,8 to measure the degree of tunneling evident in a reaction mechanism. Heavier particles alter values within the quantum harmonic oscillator and lead to slower rates, which can be both calculated using our model and measured experimentally.


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Western Hummingbird Partnership Project Continued
Posted on June 10, 2016
This week I tested some of the apps that we are considering using for our project. I went out to a trail to look for hummingbirds. The characteristics of hummingbirds that people love (small and fast-beating wings), are also the qualities that make it hard for me to find and photograph them. Also, the other aspect of our hummingbird project that we expect citizen scientists to do is to identify plants, which is a very difficult thing to do. So, we still have to work out the kinks with that. This is all still in the development stage, but it's pretty cool to help set up such a big project. Stay tuned folks!


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Transportation: Car, Cyclo
Departure: 4h00pm - Return : 7h30pm
· Cyclo around HCMC.
· Dinner on the board
A spectacular sunset adds a whole new dimension to this fully narrated, 120 minute sightseeing tour. With this cruise you'll be introduced to all the history, sights and get to know about Vietnamese traditional instruments
Come cruise with us aboard the most elegant luxury cruise in all of Saigon. You will experience the spectacular homes, breathtaking sites and natural beauty of famous Saigon River and Saigon by night from Cruise. Relax in our fully enclosed and air-conditioned interior salons or bask in the warmth of the sun in the fresh-air outdoor seating. Enjoy the beverage of your choice from either of our two full-service bars and unwind as we cruise the calm waters. If you join us for Sunset cruise or Dinner cruise you'll enjoy a delicious meal prepared fresh daily, right in our galley. The cruise conveniently located near downtown Saigon. Join us for one of our popular public cruises where you're sure to have the experience of a lifetime. We also invite you to arrange a private charter to make that special occasion unforgettable.
We depart at 4.00pm by cyclo for city sightseeing and then go to Bach Dang port for Bonsai, Indochina Junk, Ben Nghe Cruise…. have a look and take some nice pictures with cruise before departure
Enjoy Vietnamese folk music or fire dancing…. After dinner, send back to your hotel. End tour say goodbye and hope to see you again.
Unit: 1000vnd


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By John B. Shoven
Demographics is a crucial box of analysis for knowing social and fiscal swap and it has attracted consciousness lately as matters have grown over the getting older populations of constructed countries. Demographic experiences help in making experience of key elements of the economic climate, delivering perception into traits in fertility, mortality, immigration, and exertions strength participation, in addition to age, gender, and race particular developments in overall healthiness and disability.Demography and the economic climate explores the connections among demography and economics, paying certain consciousness to what demographic traits can demonstrate concerning the sustainability of conventional social protection courses and the bigger implications for fiscal development. the amount brings jointly a number of the top students operating on the border among the 2 disciplines, and it presents an eclectic review of either fields. individuals additionally provide deeper research of numerous matters comparable to the impression of larger wealth on offerings approximately marriage and childbearing and the results of getting older populations on housing costs, Social safeguard, and Medicare.
Read Online or Download Demography and the Economy (National Bureau of Economic Research Conference Report) PDF
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Extra resources for Demography and the Economy (National Bureau of Economic Research Conference Report)
Sample text
Jones, Schoonbroodt, and Tertilt (chapter two, this volume) provide a broad review of the assumptions that are required in order to generate such predictions. We expect that the median value of houses, an indicator of the housing price structure, will be negatively related to fertility because children are space intensive. We also expect fertility to be negatively related to an area's level of unemployment. The model that we estimate includes individual-level variables whose values were established in childhood: mother's educational attainment (that is, the mother of the woman interviewed in NSFG); the religion in which a woman was raised, if any; and whether or not her parents' marriage was intact when she was aged eighteen.
Edin. 2003. Differences in family attitudes by education. Presented at the annual meetings of the Population Association of America. Minneapolis, MN. Schoen, R. 2004. Timing effects and the interpretation of period fertility. Demography 41 (4): 801–19. , Y. Kim, C. Nathanson, J. Fields, and N. M. Astone. 1997. Why do Americans want children? Population and Development Review 23 (2): 333–58. Schultz, T. P. 1994. Marital status and fertility in the United States: Welfare and labor market effects. Journal of Human Resources 29 (2): 637–69.
Fertility of American women: June 2004. Current Population Report no. P20-555. S. Census Bureau. ———. 2005b. Statistical abstract of the United States: 2004–2005. S. S. Census Bureau. ———. 2007b. Historical income tables. P38. gov. ———. 2008. Historical income tables. html. S. National Center for Health Statistics. 2005a. Births: Final data for 2003. Vital and Health Statistics Series 54 (2). S. Department of Health and Human Services. ———. 2005b. S. women: Data from the 2002 national survey of family growth.