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Surgery-associated adverse events of Argus II retinal prosthesis system
Search Medline for
|November 30, 2017
Background: The Argus II Retinal Prosthesis System (Second Sight Medical Products Inc., Sylmar, CA, USA) is an implantable device designed to improve vision in patients with degenerative diseases, e.g. retinitis pigmentosa. The device has received commercial approval by the European Union (CE Mark) in March 2011 and by the Food and Drug Administration (FDA) in February 2013.
Methods: Implanting the Argus II retinal prosthesis represents a new aspect in vitreoretinal surgery bringing along new challenges in surgical procedures. First, the encircling band of the extraocular portion of the device is fixed similar to a scleral buckle and the case with the electronics is carefully positioned around the eye. Second, a sklerotomy is made parallel to the limbus. Afterwards, the array is inserted through the sclerotomy and is fixed on the central retina by using a retinal tack. Two patch grafts are used to cover the electronic cases in order to reduce the adverse events like infection and conjunctival erosion. At the end of the surgery the complete closure of the conjunctiva is crucial.
Results: Since the study's beginning surgery-associated adverse events including conjunctival dehiscence, hypotony and endophthalmitis could be reduced because of the refinement of the surgery and the device itself. But conjunctival erosion, retinal detachment and vitreous haemorrhage are still adverse events which appear and could not be reduced. By now the device has been implanted in over 230 patients worldwide, in 37 patients in Germany.
Conclusion: Implantation of the Argus II prosthesis system and the use of the device have delivered promising results and have highlighted new challenges. As the surgery will be optimized and is performed by more and more experienced surgeons, the decrease of surgery-associated adverse events can be estimated.
Acknowledgment: This project was supported by Second Sight.


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2 men charged with murder in dragging death of teenager
Officials said Kemoni Miller, 18, died Wednesday after spending four days on life support.
Two men who allegedly dragged 18-year-old Kemoni Miller with a car while robbing him last week now face murder charges after Miller died Wednesday from his injuries.
Dejon Barnes, 18, of Dorchester, and Kenneth Ford, 23, of Roxbury, were initially charged with unarmed robbery following the Nov. 16 incident in Dorchester, according to a statement Friday from the Suffolk County District Attorney's office and Massachusetts State Police.
Miller died at Boston Medical Center Wednesday morning after spending four days on life support, officials said. Charges against Barnes and Ford were updated later that day.
Investigators believe Miller negotiated to sell an iPhone XS to Barnes online before meeting with him and Ford — who were in a car rented by one of Ford's friends — on Gallivan Boulevard.
"From the passenger's seat, Barnes allegedly took the phone and closed his window on Miller's arm as Ford accelerated at a high rate of speed," officials said. " Miller was dragged about half a mile and suffered a life-threatening head injury when he fell from the car."
Miller never regained consciousness, prosecutors said.
Investigators determined Barnes offered to buy the phone while Ford drove the car by reviewing "cell phone records, text messages, witness statements, video footage, and other evidence," according to authorities. The two were arrested at a West End party early Sunday morning.
Barnes and Ford were held on $100,000 cash bail during their arraignments Monday on the unarmed robbery charge.
Their arraignments for the murder charges had not yet been scheduled as of Friday morning, but both men are expected to appear in court on Dec. 4, prosecutors said.


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Kelly Rivas explores Bangkok's forests in her Daylite Hiker
Posted on 21st Sep 2018
From exploring the busy Bangkok streets and parks, to hiking through beautiful forests in Thailand, the Daylite Hiker surely lives up to its name.
I finally bit the bullet, after months of contemplating whether I should buy a pair of Xero shoes, and I truly do love these shoes! I haven't wanted to take these off since they arrived in the mail and now wear them everywhere. I've been wearing other barefoot/minimalist style shoes but nothing compares. The Xero daylite hikers are extremely lightweight, super comfy and water resistant. My feet stayed dry while hiking in Thailand, where I crossed creeks and walked through muddy tracks. I simply ran them under water to give them a quick clean that night and then they were good as new again.
I keep getting compliments on the daylite hikers by friends and people at work now. I seriously only need one pair of shoes when travelling now!'
Xero Shoes - How to Plan your next family trip.
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Chris Newby finishes the McLaren Vale Half Marathon in his Z-Trails
Just finished the McLaren Vale (South Australia) half marathon in my new z-trails. Happy with my time of 1:45, which made me the fastest - albeit only - guy in sandals there.
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- Open Access
Highly efficient production of Clostridium cellulolyticum H10 d-psicose 3-epimerase in Bacillus subtilis and use of these cells to produce d-psicose
© The Author(s) 2018
- Received: 13 August 2018
- Accepted: 23 November 2018
- Published: 28 November 2018
d-Psicose 3-epimerase (DPEase) catalyzes the isomerization of d-fructose to the rare sugar d-psicose, which may help prevent obesity, reduce blood sugar and blood fat, and inhibit intra-abdominal fat accumulation.
In this study, the DPEase of Clostridium cellulolyticum H10 was expressed in the food-grade host Bacillus subtilis. Optimization of the culture medium during shake-flask experiments yielded a DPEase activity of 314 U/mL. The optimal medium included 20 g/L peptone, 15 g/L corn steep powder, 5 g/L glycerol, and 1 mM Ca2+. Controlling the carbon source concentration was important because elevated concentrations can result in catabolite metabolic suppression (CCR). To avoid CCR and increase DPEase expression, we developed a fed-batch strategy in a 3.6-L fermenter. We altered the ratio of carbon source to nitrogen source (C/N) in the feeding medium and employed a constant feeding rate (6 g/L/h). This strategy improved the DPEase activity to 2246 U/mL (7.8 g/L), which is almost 15 times higher than that observed in the original shake-flask cultures. Finally, we used the DPEase-expressing B. subtilis cells to produce d-psicose from d-fructose, and a 28% conversion yield was achieved with these cells, demonstrating their potential use in d-psicose production.
This is the first report to enhance recombinant DPEase production in B. subtilis using efficient and convenient fermentation strategy, and the DPEase yield is three times higher than the highest yield reported to date. The recombinant B. subtilis cells were further used in the efficient synthesis of d-psicose. This study provides a basis for the industrial production of d-psicose.
- d-Psicose 3-epimerase
- Bacillus subtilis
- High-cell-density fermentation
- C/N ratio
- d-Psicose
The International Society of Rare Sugars first proposed the widespread application of rare sugars in human nutrition and health at its first academic conference in 2001. There are currently 34 species of rare sugar or sugar alcohol under consideration, including d-psicose, d-tagatose, d-allose, and xylitol. d-Psicose, an epimer of d-fructose at the C3 position, is 70% as sweet as sucrose, but its caloric value is only 0.3% of that of sucrose [1–3]. d-Psicose has recently attracted considerable attention from researchers because it has been linked with several useful activities, which include preventing obesity, reducing blood sugar and blood fat, and inhibiting intra-abdominal fat accumulation [4–6]. Because of its good physical and chemical properties, d-psicose has been used in food, dietary supplements, and pharmaceutical preparations, and has enormous market prospects [7].
DPEase is the key enzyme required to produce d-psicose from inexpensive d-fructose, therefore it is important to identify an active enzyme and improve its yield. DPEases from many sources have been discovered over the last few years, and most of them have been expressed as recombinant proteins in Escherichia coli [11–13]. These methods are useful for experimental purposes. However, industrial d-psicose production is meant to produce a food additive, so safety issues are particularly prominent. Due to the production of endotoxin, E. coli as a host presents a security problem and is not suitable for the expression of DPEase intended for use in the industrial production of d-psicose. B. subtilis, a food safety bacterium belonging to the group of microbes classified as generally regarded as safe (GRAS), widely used in the production of industrial enzymes due to absence of security issues [14, 15]. Therefore, the overproduction of heterologous proteins by high-cell-density fermentation in recombinant B. subtilis is highly valued. Since the culture medium and cultivation conditions may have substantial effects on protein production, it is important to determine the optimal conditions for the production of each particular protein in B. subtilis. During typical industrial fed-batch fermentations of B. subtilis, efficient protein expression often requires maintaining the carbon concentration at a low level in the medium. For example, Zhu et al. studied different glucose feeding approaches and step-wise dissolved oxygen control methods to improve N-acetylglucosamine production [16] and Shene et al. increased the recombinant enzyme concentration by feeding the carbon source slowly during fed-batch fermentation [17]. Huang et al. reported that high initial glucose concentrations cause product inhibition and used a dual exponential fed-batch strategy to prevent this inhibition [18]. However, this method is tedious and requires sophisticated control measures. Other components in culture medium might also have a significant effect on protein production. Chen et al. found that glucose and glycerol were not conducive to the expression of recombinant B. subtilis Rluc protein, and a high protein yield was obtained by reducing the amount of glucose and adding yeast extract in a 5-L fermenter [19]. Kwon et al. found that when the ratio of glucose to peptone in the feeding medium was 0.33:1, the activity of recombinant nattokinase reached 14,500 U/mL, which was 4.3 times higher than that in batch culture [20].
The past few years witnessed a number of reports describing the recombinant expression of DPEases in B. subtilis [21–23]. However, to the best of our knowledge, only a few studies have investigated DPEase expression levels in both B. subtilis and other hosts. In 2016, Sun and coworkers constructed a recombinant DPEase expression vector containing a xylose-inducible promoter that successfully expressed DPEase in B. subtilis [24]. Using this system, the expression level of DPEase reached 95 U/mL (2.6 g/L) in a 7.5-L fermenter with the fed-batch fermentation. To our knowledge, this was the highest production level prior to this study.
In the present study, a synthetic, codon-optimized gene encoding the DPEase from Clostridium cellulolyticum H10 was successfully expressed in B. subtilis strain CCTCC M 2016536 [25] using a vector containing the constitutive promoter PamyE. Initial experiments to optimize DPEase production were carried out in shake flasks. Further efforts to improve DPEase expression involved high-density culture carried out in a 3.6-L fermenter. The fed-batch fermentations were improved by optimizing the C:N ratio of the feeding solution. Finally, we studied the synthesis of d-psicose by whole-cell catalysis with varied wet cell dosage, temperature, and pH, respectively.
Materials and methods
Bacterial strain and vectors
The codon-optimized (based on B. subtilis preferences) gene encoding the DPEase from Clostridium cellulolyticum H10 was synthesized to Generay Co., Ltd (Shanghai, China). It was inserted in B. subtilis–E. coli shuttle vector pHY300PLK (Takara, Dalian, China) and expressed in B. subtilis CCTCC M 2016536 (constructed and stored by our laboratory).
Shake-flask cultures
The Luria–Bertani (LB) medium contained (g/L) yeast extract 5.0, tryptone 10.0, and NaCl 10.0 was used during the production of seed cultures. The Terrific Broth (TB) medium contained (g/L) tryptone 12.0, yeast extract 24.0, glycerol 5.0, K2PHO4 12.5, and KH2PO4 2.3 was employed for protein expression. Seed cultures were initiated by inoculating 10 mL of LB medium supplemented with 20 mg/L tetracycline in a 50-mL shake flask with 20 μL of glycerol stock strain. These seed cultures were cultivated at 37 °C in a rotary shaker at 200 rpm for 8 h. Then, 2.5 mL of the seed culture was transferred to 50 mL of TB medium supplemented with 20 mg/L tetracycline in 250-mL shake flask. This culture was shaken at 200 rpm for 48 h at 33 °C.
Media, feeding solutions and cultivation in 3.6-L fermenter
Seed cultures were prepared in LB medium as described in the previous section. A modified semisynthetic medium was used for cultivation in a 3.6-L fermenter. The initial batch medium contained (g/L) peptone 20.0, corn steep powder 15.0, glycerol 5.0, K2PHO4 12.5, KH2PO4 2.3, (NH4)2-H-citrate 1.0, (NH4)2SO4 2.7, MgSO4·7H2O 1.0, and CaCl2 0.2, as well as 3 mL/L trace metal solution [26]. The feeding solution contained 500.0 g/L total glycerol and nitrogen (industrial grade), 7.9 g/L MgSO4·7H2O 7.9, and 20 mL/L trace metal solution.
The initial batch medium for fed-batch cultivation (0.9 L) in a 3.6-L fermenter (Labfors 5, Infors-HT Co., Ltd) kept at 33 °C and pH 7.0 was supplemented with 20 mg/L tetracycline and then inoculated with a 100-mL seed culture. The pH was controlled with 20% phosphoric acid and ammonium hydroxide. When the glycerol present in the initial medium was consumed, as indicated by a sudden increase in the dissolved oxygen and a decrease in the agitation rate, we began adding the constant feeding solution to the culture. To maintain the dissolved oxygen level at about 30% of air saturation during the fermentation process, the agitation speed was cascaded between 200 and 700 rpm.
Determination of biomass
Cell growth was monitored by measuring the optical density of the culture broth at 600 nm (OD600) using a spectrophotometer (BioPhotometer plus, Eppendorf Co., Hamburg, Germany). To determine the dry cell weight (DCW), 5 mL of culture broth was centrifuged at 12,000g for 10 min. The pelleted cells were washed twice with 0.9% (w/v) NaCl and centrifuged at 12,000g for 10 min, then dried to constant weight at 105 °C.
Preparation of DPEase and SDS-PAGE
A sample of the culture broth was diluted to an OD600 of around 5 and centrifuged at 12,000g for 10 min. The pelleted cells were resuspended in 1 mL HEPES buffer (20 mM, pH 8.0), then the cells were incubated with 30 μL lysozyme (20 mg/mL) at 37 °C for 30 min before disruption by sonication for 10 min (pulse on for 2 s and pulse off for 3 s). The cell debris was removed by centrifugation at 12,000g for 5 min.
The protein content of the culture broth was assessed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Protein bands were stained with Coomassie Brilliant Blue R-250 dye according to standard procedures.
DPEase activity assay
DPEase activity was measured via determining the quantity of produced d-psicose. The reaction mixture (1 mL final volume) contained d-fructose (80 g/L), HEPES buffer (20 mM, pH 8.0) and Co2+ (0.1 mM), and the reaction was initiated by the addition of enzyme solution (200 μL). The reaction was incubated at 55 °C for 10 min, and then stopped by placing the tube in boiling water for 10 min. The amount of generated d-psicose was determined by using a high-performance liquid chromatography (HPLC) method. The HPLC apparatus was equipped with a Shodex™ Asahipak™ NH2P-50 4E column and a refractive index detector (Agilent Technologies, Palo Alto, CA, USA). The column was maintained at 35 °C and eluted with 75% acetonitrile at a flow rate of 0.8 mL/min. One unit of DPEase activity was defined as the amount of enzyme that catalyzed the production of 1 μmol of d-psicose per minute.
Results and discussion
Recombinant DPEase expression in B. subtilis
Effects of process conditions on the production of DPEase in shake flasks
Nitrogen sources and nitrogen source concentrations
The effect of different nitrogen sources on cell growth (DCW) and recombinant DPEase activity in shake flasks
Nitrogen source
Concentration (g/L)
DCW (g/L)
DPEase activity (U/mL)
Yeast extract
4.1 ± 0.1
137.6 ± 2.8
5.2 ± 0.2
162.0 ± 3.4
5.3 ± 0.1
127.7 ± 3.2
4.1 ± 0.1
164.1 ± 3.5
5.1 ± 0.1
189.9 ± 2.8
5.4 ± 0.2
186.4 ± 2.6
Yeast decoction
3.2 ± 0.1
122.4 ± 3.9
3.4 ± 0.1
128.4 ± 2.9
4.0 ± 0.1
142.6 ± 3.5
Corn steep powder
2.6 ± 0.1
92.4 ± 1.9
4.3 ± 0.1
167.0 ± 3.3
4.4 ± 0.1
111.1 ± 2.1
5.7 ± 0.2
109.0 ± 2.9
Cottonseed meal
3.2 ± 0.1
123.8 ± 2.7
4.7 ± 0.2
199.4 ± 3.9
6.2 ± 0.2
213.7 ± 4.8
Carbon sources and carbon source concentrations
To determine the optimal glycerol concentration for DPEase production, glycerol with concentration varying from 1 to 25 g/L was added to the culture medium, respectively. As shown in Fig. 4b, the highest DPEase activity (259.7 ± 7.8 U/mL) was obtained with 5 g/L glycerol. When the glycerol concentration was increased, the DCW and enzyme activity decreased significantly, and the final pH in the fermentation medium also declined. These changes may result from catabolite metabolic repression (CCR), which has been noted during B. subtilis fermentation [27]. In CCR, the presence of an abundant carbon source has adverse effects on cell growth and recombinant protein production. Catabolite control protein A (CcpA) is the primary regulatory protein that mediates the CCR effect. CcpA binds to specific DNA sequences (CRE boxes) and inhibits gene transcription, thereby repressing protein production [28]. The expression vector used in this study contains a CRE sequence upstream of the PamyE promoter. The presence of the CRE sequence may cause an increase in carbon source concentration to significantly decrease DPEase expression. The CCR effect also alters metabolic pathways and can lead to the accumulation of acetic and lactic acids. Thus, the CCR effect would be expected to decrease the pH of the fermentation medium with increasing carbon concentration, which is exactly what is observed (Fig. 4b).
Metal ions
The effects of different ratios of feeding carbon source to nitrogen source on the production of DPEase in 3.6-L fermenter
The fed-batch cultivation strategy is often used to obtain high cell density and protein expression. When implementing this strategy, several parameters (medium component and concentration, temperature, pH, dissolved oxygen, et al.) must be optimized to increase target protein production [29–31]. The shake-flask experiments described above confirmed that the carbon source concentration has a substantial influence on cell growth and DPEase production and that elevated carbons source levels lead to a CCR effect. Strategies to minimize the CCR effect in B. subtilis have included adjusting the glucose flow rate while monitoring the pH and carbon concentration [32] and maintaining the glucose concentration at a low level [33]. In our previous work, when we maintained the carbon source concentration below 1 g/L during fed-batch fermentation in a 3.6-L fermenter, the yield of recombinant DPEase in B. subtilis was only 503 U/mL (1.7 g/L). We also tried controlling the feeding rate during different growth stages as well as adjusting the fermentation temperature, pH, dissolved oxygen content and other parameters. However, the results were still not ideal. As an alternative to previous approaches, we decided to manipulate the ratio of carbon to nitrogen (C/N) while controlling the feeding medium concentration at 500 g/L to ensure the needs of nutrition. To test this approach, fed-batch experiments were used to assess the effects of different C/N ratios on DPEase production.
The effects of different C/N ratios on cell growth and DPEase activity in fed-batch fermentation are summarized in Fig. 6f. DCW (85 g/L) and DPEase activity (2246 U/mL; 7.8 g/L) were highest with 1:2 ratio of C/N, which was the optimal condition for DPEase production via high-density fermentation. This DPEase activity level was 15 times higher than that seen in shake-flask fermentations and 4.5 times higher than that obtained by maintaining the carbon source concentration below 1 g/L using conventional fed-batch fermentation techniques. The final recombinant DPEase production level was also threefold higher than the highest level reported to date.
To the best of our knowledge, this is the first report to enhance recombinant DPEase production in a food-grade expression system using an efficient and convenient fermentation strategy. In this study, the feeding rate (6 g/L/h) remained constant during the fermentation period, eliminating the need to adjust the feeding rate to maintain a low carbon source concentration. Thus, we could obtain high protein production using a process that is easy to control and conducive to industrial applications.
Bioconversion of d-fructose to d-psicose by recombinant B. subtilis cells
DPEase is an intracellular enzyme. To obtain substantial quantities during industrial production, cells must be lysed and the recombinant enzyme purified, which is both time-consuming and expensive. The substrate (d-fructose) and product (d-psicose) of DPEase are small molecules that easily enter and exit cells. Bioconversion of d-fructose to d-psicose by whole-cell catalysis has the potential to avoid DPEase extraction and purification, improve enzyme stability, and meet the needs of industrial d-psicose production. Therefore, we assessed the use of our DPEase expression system to produce d-psicose directly. The effects of wet cell dosage, temperature and pH on d-psicose production were investigated.
The DPEase from C. cellulolyticum H10 was expressed in Bacillus subtilis, and fermentation conditions were optimized to improve DPEase production. A feeding strategy that combined a low carbon substrate concentration with a constant feeding rate in a 3.6-L fermenter was proposed. The resulting DPEase expression level (2246 U/mL; 7.8 g/L) achieved was three times greater than the highest level reported to date. The recombinant B. subtilis cells were further used in synthesis of d-psicose, and the conversion rate of 28% was achieved. This study provided a simple and convenient strategy to minimize the CCR effect in recombinant B. subtilis fermentation and established a basis for industrial scale production and application of DPEase.
Authors' contributions
JW supervised the project and revised the manuscript, LQS carried out the experiments and drafted the manuscript, FS, and KZ carried out the experiments. ZZL revised the manuscript. All authors read and approved the final manuscript.
Competing interests
The authors declare that they have no competing interests.
Availability of data and materials
The dataset supporting the conclusions of this article is included in the article.
Consent for publication
Not applicable.
Ethics approval and consent to participate
Not applicable.
This work was funded by grants from the Natural Science Foundation of Jiangsu Province (BK20180082), the National Science Fund for Distinguished Young Scholars (31425020), the National Natural Science Foundation of China (31501419, 31771916), the national first-class discipline program of Light Industry Technology and Engineering (LITE2018-03), and the 111 Project (No. 111-2-06).
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- Zhang K, Duan X, Wu J. Multigene disruption in undomesticated Bacillus subtilis ATCC 6051a using the CRISPR/Cas9 system. Sci Rep. 2016;6:27943.View ArticleGoogle Scholar
- Wenzel M, Muller A, Siemann-Herzberg M, Altenbuchner J. Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation. Appl Environ Microbiol. 2011;77:6419–25.View ArticleGoogle Scholar
- Bruckner R, Titgemeyer F. Carbon catabolite repression in bacteria: choice of the carbon source and autoregulatory limitation of sugar utilization. FEMS Microbiol Lett. 2002;209:141–8.View ArticleGoogle Scholar
- Hueck CJ, Hillen W. Catabolite repression in Bacillus subtilis: a global regulatory mechanism for the Gram-positive bacteria? Mol Microbiol. 1995;15:395–401.View ArticleGoogle Scholar
- Deka D, Das SP, Sahoo N, Das D, Jawed M, Goyal D, Goyal A. Enhanced cellulase production from Bacillus subtilis by optimizing physical parameters for bioethanol production. ISRN Biotechnol. 2014;4:2013.Google Scholar
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- Park YS, Kai K, lijima S, Kobayashi T. Enhanced β-galactosidase production by high cell-density culture of recombinant Bacillus subtilis with glucose concentration control. Biotechnol Bioeng. 1992;40:686–96.View ArticleGoogle Scholar


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An injection of federal green energy money will make a highrise in the heart of the province's first net-zero community — one that produces as … Read More
2 Guelph Developments Recommended For Approval
City staff are recommending that two development proposals get the green light – one for the second time. According to reports to go before councillors for … Read More
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LFP Longform: Highrise Building Boom Will Transform London's Skyline
The highrise building boom in London shows no sign of slowing, city hall building officials say. The city has seven applications for apartment building permits … Read More
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A new apartment building could be coming to the city's south end. At Thursday evening's meeting of the committee of adjustment, members unanimously approved a … Read More
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Downtown Kitchener could see a construction boom in about two years as developers rush to meet a deadline that could save them millions of dollars … Read More
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It's shaping up to be a stellar year for London's housing industry, with new construction up almost 30 per cent in the first half of … Read More
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Jonathan Adler, the Johan Verheij Memorial Professor of Law and director of the Center for Business Law and Regulation, is the eighth most-cited public law scholar—excluding constitutional law—in the U.S., according to research conducted by University of Chicago law professor Brian Leiter.
Adler is the only scholar among the top 10 under 50 years old; he is one of just four scholars under 50 on the list. Between 2013 and 2017, the period for the ranking, Adler was cited 620 times.
See the full list on Leiter's Law School Reports website.


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Laurent Blanc's men are unbeaten in their last 20 competitive matches but, having already qualified for the knockouts, could relax a little more against the Dutch champions
Ajax are quietly confident they can be the team to end Paris Saint-Germain 's 20-match unbeaten run in competitive games.
The two sides meet in the French capital on Tuesday in Group F of the Champions League, with the hosts' progression to the last 16 already secured.
PSG have won three of their four matches in the group stages – their only blemish being a 1-1 draw at Ajax in September.
That sees them top the pool with 10 points – one ahead of Barcelona – while Ajax sit third and are still after their first victory.
PSG can win the group if they claim victory and Barcelona lose at APOEL. For Frank de Boer's men, who cannot progress in Europe's premier competition, victory would at least confirm third.
With that comes a place in the Europa League and it is a position that the four-time defending Eredivisie champions are targeting.
After a 4-1 league win over Heerenveen on Saturday that saw them keep the pressure on leaders PSV, Anwar El Ghazi is quoted as saying on the club's official website: "Now we have won, the good work [continues] towards Paris.
"I think there [we can] definitely get something.
"We just need to be third [in the group]…it is an advantage that Paris is already [qualified]."
Ajax trailed 1-0 with just 24 minutes remaining but were greatly benefited by Luciano Slagveer's red card and an own goal from Daley Sinkgraven.
El Ghazi, Ricardo Kishna and Arkadiusz Milik all scored in the latter stages for Ajax and although the performance was scrappy, midfielder Davy Klaassen shared his team-mate's enthusiasm.
"On the basis of today, we are not ready for Tuesday's game. But I'm quite confident," he said.
PSG almost had their winning streak, which now stands at seven matches, brought to an end against Metz on Saturday.
Laurent Blanc's men led 2-0 in the early stages but two Modibo Maiga penalties set up a nervy finish.
Ezequiel Lavezzi, who had earlier scored in the first half, was on hand to net an 83rd-minute winner for PSG, though, as they triumphed 3-2 in Zlatan Ibrahimovic's return to the starting line-up.


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The Permanent Maternal Record
Or: Life (and death) as we know it
Eos remembers: "One day, when I was 5 years old, I decided to run away. Mom had scolded me for something, and my feelings were hurt.
"When I announced I was running away, Mom said she was sorry to hear that. She told me to be careful, and reminded me I couldn't go any farther than Chippewa Avenue, because she and Dad didn't want me to be hit by a car.
"I got on my tricycle, rode up Valley Lane, and looked back at the house. I didn't see anyone coming to apologize to me, and that made me sad.
"I pedaled down View Lane with tears in my eyes. Nobody cared!
"When I got to Butler, I got homesick, so I went back home. Mom was there with a hug. She'd been watching me the whole time, to make sure I was safe.
"Love stretched far enough to let me test it.
"If I could, I'd give her the world's biggest hug. I miss my mama."
'Tis the season!
Gustatory Division
Cheesehead By Proxy, "back in Northern Minnesota": "You asked for old favorite recipes, and I still have this Bulletin Board recipe from way-back-when tucked into my recipe box.
"Any child of the 1960s who loved the 'pizza burgers' we had for school lunch at Mounds View High School will love these roll-ups.
"Or if you want the real deal, spread the Spam mixture onto a half hamburger bun, top with some shredded mozzarella cheese, and bake or broil until cheese melts and bubbles.
"I can still see the little green plastic hot-lunch tokens (shaped like tiny stop signs) with which we purchased our hot lunches. Milk tokens were white.
"Just about everyone ate hot lunch on pizza-burger days. Some of the hungry teenage boys would buy double lunches in order to get four half-bun pizza burgers rather than settle for two.
"These are so good that one can actually forget they're made from ground Spam!"
The Astronomer of Nininger: "Subject: The Incredible, Edible Village.
"For the past 38 years, The Good Wife and I have hosted a Christmas party for family and friends. It has grown by leaps and bounds; on some occasions, more than 100 people show up. And each year we do something different and special. One fabulous feature of the gathering is the Incredible, Edible Village!
"It began eight years ago with construction of a few gingerbread houses, forming a Christmas-themed hamlet. For the past couple of years, The Good Wife has invited neighbor 'Bee in the Wool' to move in for a weekend, with the express purpose of together investing enough time on task to create a more spectacular village than that of the preceding year. The really neat thing is that everything is edible — from candy canes to sugar drops, pretzels and lollipops, chocolate kisses and candied cherries and all kinds of delicious delights. Imaginations go wild, and creativity knows no limits.
"Here are some images of the village and the animals currently residing therein:
"Some polar-bear inhabitants of the winter wonderland; made from white-chocolate-dipped Oreo cookies
"Reindeer readying for Santa's sleigh, crafted out of Nutter Butter cookies
"An igloo out of Rice Krispie balls smothered in marshmallows.
"The local church, central to the village. Gingerbread and yummy frostings.
"One of the country mice: chocolate-dipped-cherry body with chocolate-kiss head and almond-sliver ears."
Till death us do part
And: Our pets, ourselves
Little Sister: "My husband thought I'd eaten them all. Miffed, I told him that I was shocked and appalled that he'd think me capable of eating a dozen freshly baked biscuits in less than 10 minutes. No matter that I was having the same accusatory thoughts about him.
"It didn't take long for our attention to turn towards our rescue dog, Tinka, curled up on the floor. Guilty eyes followed our every move, as she released a contented burp. 'It isn't as though I ate them ALL,' she seemed to say. 'I did leave six behind for you!'
"Having to forage for her food the first year of life, Tinka has maintained some of those thoroughly honed doggie habits that helped her survive on her own — one of those being that a wise dog never passes up an opportunity to eat, because you never know when another one will come your way.
"With advice from the vet to keep an eye on her, we settled in for some unsettling time spent making another batch of biscuits and waiting for her to explode, or purge on the living-room carpet. She didn't. In fact, much to our relief, she came through her gorge unscathed. (I think the dog could digest nails if she had to.)
"Lessons learned: Never underestimate the length of Tinka's reach, or her determination. And never leave biscuits to cool unattended on the kitchen counter."
Verbing of America
Rusty of St. Paul: "My wife and I decided to have four friends over for supper on a somewhat spur-of-the-moment thing. When we have people over, we tend to make a fuss over the event, with a clean house and elegant food (as I enjoy planning a meal, buying the ingredients and doing the cooking).
"Just as we finished breakfast and were getting ready for a day of thorough cleaning, provision buying and cooking, my mother-in-law called to ask about an issue with my father-in-law's eye. I am a retired emergency-room provider and told her I'd be right over. I was hopeful to avoid a long ER stay, but after doing an exam and getting in touch with the on-call eye doctor, the advice was to go to the ER — ASAP.
"It was an hours-long stay, but at one point I was able to sneak out and leave my wife with her dad so as to buy food. Cleaning? All I had time to do was to throw loose 'stuff' into boxes, move them into a back room and upstairs, and close the doors. When I picked my wife at the ER, I told her I had 'Fibber McGee'd the house.'"
The sign on the road to the cemetery said 'Dead End'
Electronic Board of the Church on Lexington in Shoreview Division
Our Official Electronic Board of the Church on Lexington in Shoreview Monitor — Red's Offspring, north of St. Paul — reports: "Subject: Fit for a King.
"The latest message on the electronic board of the church on Lexington in Shoreview:
'Tis the season! Celluloid Division
Plus: Where've you gone, Mrs. Malaprop? Leading to: The great comebacks (missed). And: Everyone's a critic!
Burywood Dave reports: "We were discussing holiday movies the other day, and 'White Christmas' was mentioned as a favorite. My daughter asked who was in it, and my wife said: 'Bing Crosberry was the star.' I suppressed a chuckle and waited a second for her to correct herself, but as no correction was forthcoming, I added: 'And don't forget Danny Kayeberry, Vera Lynberry, and Rosemary Clooneyberry.' All I got was a dirty look. (Bulletin Board says: Too bad! Had we been your wife, we'd have said: "Don't you mean Vera-Ellenberry . . . Mr. Smart Guy?" LOL.)
"And as long we're on the subject: Am I the only one who is just a little irritated at how things unfold in 'It's a Wonderful Life'? First of all, Potter gets away with a serious crime, Uncle Billy will always feel the guilt and pain and be regarded as the family idiot, and the mystery of the missing money will forever hang like a dark cloud over the Bailey household. I've always pictured a final scene where Potter is being wheeled up the walk to the Bailey house. In his lap is the infamous newspaper that held, and now again holds, the missing money. He pauses and listens to the music and laughter coming from inside, then says to his aide: 'All right, let's get this over with.' And there it would end. Potter comes clean, money is returned, and loose ends are tidied up.
"Merry Christmas."
What is right with people?
Email: "Will Shapira of Roseville notes that whenever he goes out for a walk, he always makes it a point to pick up trash and dump it in a convenient receptacle. However, since receptacles aren't always handy, Will takes as much trash as he can carry and dumps it when he returns home.
"Will notes that not along ago, he was walking along the west side of Lexington Avenue across from Roseville Central Park, picking up trash along the way, when a woman ran up to him and handed him a paper bag. 'Here! This will make it easier for you!' That it did, says Will, but before he could thank her, she was gone.
"Who says people are no damn good?"
BULLETIN BOARD SAYS: No one we spend any time with, if we can help it.
Our community of strangers
And: Where we live
Bloomington Bird Lady writes: "Subject: Appreciate being a Minnesotan!
"Do you Bulletin Boarders remember the first time you wrote a story for the Pioneer Press? Having an outlet for one's creativity, and then hoping to see the story in print? Pretty exciting, actually. Now with the blog online, it is still such a good way to communicate and hopefully hone writing skills by seeing 'story' possibilities in the oddest or even the most ordinary places.
"One of my first pieces was about my friend, a Holocaust survivor, whose story reminded me of how much I loved Anne Frank's discovered diary. It had become my favorite book, partly because it was written in the 'first person,' which tends to quickly grab the reader right from Page 1. I have since reread that book several times, even lately, as I turned 86. It is true, and a page-turner without using the 'F' word that the present writers seem to enjoy sprinkling lavishly throughout their detective novels. (Bulletin Board interjects: We've lately been checking out "The Marvelous Mrs. Maisel" on Amazon and have wondered, numerous times, whether people in the late 1950s and early 1960s — even standup comedians and their managers — used that same "F" word as relentlessly as the "Mrs. Maisel" characters do.)
"Through the years, our friendship has grown even though we live quite far from each other. She's up north of St. Paul, and here I am in Bloomington, but we call each other every Saturday afternoon at 5 p.m. without fail. This month, she will turn 93, and we got to celebrate our birthdays together this week. She made French onion soup, which is her specialty.
"She often says: 'I owe my life to so many angels who have helped me ever since I came to this country. I would not be here if it weren't for them.' She has become an excellent American citizen and loves Minnesota dearly.
"We can appreciate our country better sometimes when seen through the eyes of one who saw Hitler take over France, her father be suddenly taken away to Auschwitz, the American liberation finally, and who was actually an orphan in Paris before finding a sponsor in America. She has so many stories of how her 'angels' saved her life.
"Birdman and I, with our daughter from Burbank, recently toured the newly renovated Minnesota State Capitol. So beautifully done, and thrilling to see.
"First thing I did after coming home was to call my friend to tell her that she just had to see our beautiful Capitol! Well, of course, she said; she always takes everyone on that tour, and why did it take us so long to get there?"
Band Name of the Day: The Family Idiots


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Dating for Young Adults With Disabilities. Published April 21, By Laura Riley. Finding one surefire approach to dating for people with disabilities is as difficult as nailing down one definition for disability. "People with disabilities are the biggest minority group in the United States," says Trevor Finneman, a year-old attorney.
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from one coordinate to uncoordination
CANADA Caduc CA08 CD (2015)
Here, we have another fine release from Mathieu Ruhlmann's Caduc label, this time a duo of Lali Barrière and Alfredo Costa Monteiro. I previously enjoyed Ruhlmann's Concert For Charles Cros with Lance Austin Olsen and Daniel Jones on Caduc. Monteiro I know from his project Atolón with Ruth Barberán and Ferran Fages, whose excellent 2013 album Concret I reviewed here: http://www.thesoundprojector.com/?s=atolon. He also works with Fages under the name Cremaster, with Barberán under the name i treni inerti, (literally translated; Inert Trains), and in duos with Pascal Battus and Tim Olive as well as solo. Lali Barrière is a Barcelona-based musician who has also worked with Ruth Barberán and Ferran Fages, along with many other notable improvisers such as Tom Chant, Xavier Lopez, Tom Soloveitzik, Dafne Vicente-Sandoval and Artur Vidal and sound artist Nuno Rebelo. She teaches mathematics as a Professor at Universitat Politècnica de Catalunya and also creative music. The scene in Barcelona seems to be particularly close-knit; Ferran Fages mastered from one coordinate to uncoordination. Monteiro produced the sleeve artwork himself.
The work itself is one piece of deep, expansive music based around unhurried movement and tonal interplay, performed on two synthesisers over a good, long duration of 73 minutes. Actually, as an admirer of long-duration improvisation, I could happily listen to much more than that; this is great stuff – immersive, subtle and enigmatic. In fact, when on the first listen, as the music finished, I immediately pressed play again and listened to the whole thing straight through for a second time. Demonstrating considerable restraint and purity of intention, from one coordinate to uncoordination keeps a fairly uniform dynamic until about halfway where Barrière and Costa Monteiro break it down into gossamer components. All the way they maintain a linearity in their interplay. Later, at 50 minutes, both synthesisers take on the timbre of church organs as if heard from outside; filtered by the stone walls of a church.
I am aware that there has been a recent resurgence of interest in all things analogue in the world of synthesis, and it is not stated on the sleeve whether the equipment being used here is digital or analogue, (however there is a video of BLAAST in performance and it looks like they are using modern desktop synthesisers, but it's too dark to make out much more than that). Purists may (and probably will) argue over this until the cows come home, but I don't really think about it either way – the music speaks for itself and, could feasibly be adapted for different instrumentation. Furthermore, those familiar with either of the musician's previous output may be surprised to hear synthesisers used at all. Both Monteiro and Barrière are normally firmly ensconced in the EAI area of music making with acoustic instruments, unconventional strategies, extended technique and so forth. Displaying steady development, from one coordinate to uncoordination avoids stagnation or unnecessary busyness. I had an emotional response to it at once, hence my urge to listen through it again straightaway.
Again with Caduc, the production values on the disc are high – full colour professional printing on a heavy card stock fold-over sleeve, with the thoughtful addition of a bookmark included.


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Kunihiko+Kasahara+-+Origami+ – Free ebook download as PDF File .pdf) or read book online for free. With more than projects of many different types and levels of difficulty, this bestselling, encyclopedic work contains traditional and modern paper-folding. 1 Jun Origami Omnibus by Kunihiko Kasahara, , available at Book Depository with free delivery worldwide.
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Mammals – Rhinocerotidae – Rhinoceros. Lion Mammals – Felidae – Lions. Decorative Origami Boxes Rick Beech. Birds – Galliformes – Hens and Pheasants. Swan Birds – Anseriformes – Swans. No, it's a real mess!
Gilad's Origami Page
Folded by Malachi Brown. Preview — Origami Omnibus by Kunihiko Kasahara. Sea anemone Cnidarians – Anemones and Jellyfish. There are no discussion kinihiko on this book yet.
Origami Omnibus by Kunihiko Kasahara Book Review | Gilad's Origami Page
Cherry blossom Flora – Flowers. I have wanted to increase my origami skills for a long time. Cube omnivus Geometric and other shapes. I believe this book to be one of kunihiko kasahara origami omnibus most important origami books ever to be published.
Pentagonal flat unit variation Geometric and other shapes. Grinning old man mask People. Crease And Fold Sok Song. Origami Japanese Paper Folding: Origami Paper Kunihiko kasahara origami omnibus Tuttle Publishing. Japanese Washi Patterns Tuttle Publishing. Celestial general mask Imaginary beings.
Origami Omnibus: Paper Folding for Everybody
Long rectangular box Boxes and Containers. Kamikara – Mechanical Origami In stock 2 copies remaining. Angie rated it it was amazing Sep 04, Birds – Pelecaniformes – Herons and Ibises.
Kynihiko highly recommend this book as well. This book has complete instructions for some models, and others the kunihiko kasahara origami omnibus leaves the finish to the reader, encouraging thought. Origami Omnibus – Revisited Kunihiko kasahara origami omnibus We have masks, Animals of every kind, realistic and more kunigiko, modular and geometric shapes, as well as discussions about mathematical, aesthetic and design aspects of origami, introduction to crease patterns, and more.
Peacock Birds – Galliformes – Peacocks. Llama Mammals – Camelidae – Llamas.
Orirobo Battle Royale Cormorant Birds – Suliformes – Cormorants and Gannets. Square flat unit – iso area Geometric and kunihiko kasahara origami omnibus shapes. Thanks to more patience and maturity I no longer look at a complex design and throw my hands up in frustration, I have the discipline to work things out. Martin rated it really liked it Apr 04, Super Simple Origami John Montroll.
Condor Birds – Falconiformes – Birds of prey. Candle Objects – Candles and Light. If you don't have this book in your collection yet, you need to get it. Goodreads helps you keep track of kunhiiko you want to read. Pteranodon Dinosaurs – Pteranodon. kunihiko kasahara origami omnibus
Origami Roses and Flowers by Naomiki It is an origami book designed more for middle of the road folders and more advanced paper lovers, dabbling in the philosophy of origami and the kawahara, more than just the fun of folding. Kunihiko kasahara origami omnibus People – Anatomic parts.
Duck Birds – Anseriformes – Ducks and Geese. Tree Flora – Trees and Plants. World's Best Origami Nick Robinson. Chicken Birds – Galliformes – Hens and Pheasants. Pinocchio mask Media and Culture. Grass Flora – Trees and Plants.
Origami Omnibus: Paper-folding for Everybody – Kunihiko Kasahara – Google Books
Every chapter is brimming with ideas and challenges. Home Contact Us Help Free delivery worldwide. Stegosaurus Dinosaurs – Stegosaurus.


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text: Pon and Zi are two adorable, emo-alien lovers who are very funny and even a bit (Did I say a bit?!) random sometimes. Their genders are unknown, as they change throughout the comics so people can relate to them better. Pon is the yellow one, and zi is blue. Anyway, enjoy these pics! If there are any that are too small to read, right-click on them and press the "save as" action. then a window should come up so then press "save," and then press the start menu and click on "my pictures", then right click on the photo you want to see and click on the "preview" option then right click on the image and choose the "zoom in" option and keep zooming until you can read it. Anyway, LET ME INTRODUCE...PON AND ZI!!!


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Printed circuit board is Printed Circuit Board; it is made of copper by etching photographer clad. It is described as printed wired board PWB. pcb assembly services Printed Circuit Aboard is Printed Circuit Board of directors without components and considering components it is designated PCB assembly i.e. live Printed Circuit Board by working with electronic components. There are merely two types of unsavory Printed Circuit Board Eligible person sided board with small track of copper level. Double sided board with two tracks of birdwatcher layer. There are what's more multi layer printed program boards that have at least two layers of photographer.
The material used is really thin copper foil available for conductive layer and heat retaining material for the base. Plenty of methods for producing Pcb such as PCB running is least used more recently as it is not too effective for the conglomeration production of Printed World Boards and hence end up being used for prototype production i really.e. production in limited numbers. Copper etching method is truly most widely used across the globe but the screen branding method is very affordable as compared to others, although it provides maybe even less quality products. The whole entire manufacturing process of Pcb is Flow of Printed circuit board Manufacturing Process is Start > imaging > imprinted > Multilayer Pressing > Drilling > Plating > Masking > Finishing > Quality Control > Diagnostic tests.


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Volume 1, Issue 5, December 2013, Page: 97-103
An Assessment of Students' Learning from Term Projects
Israel Dunmade, Environmental Science Dept., Mount Royal University, Calgary, AB T3E 6K6, Canada
Received: Aug. 30, 2013; Published: Dec. 20, 2013
DOI: 10.11648/j.sjedu.20130105.17 View 2261 Downloads 80
The purpose of this study is to assess students' learning from term projects. A term project is a hands-on assignment that require students to apply ideas, theories, concepts, or principles learned or studied in class to a new situation/real life scenario. Term projects in ENVS 4413 Principles of eco-industrial development are designed to help students connect theory with practice. The aim is to enhance students' learning of the course materials and to give them experiential knowledge of how the course materials are applied in the workplace. A qualitative method was used in the study. Term project reports, midterm exam papers and final exam papers submitted by students were the sources of data used in assessing students' learning from the term project. Term project reports and exam papers submitted by students were assessed in terms of solutions suggested for the problem situation, eco-industrial theory applied, the relevance of the theory applied, and the degree of effectiveness of the student in using the theory to profer solution to the problem. Data analysis showed that 87.5% of students in the class accurately diagnosed the problem, used appropriate eco-industrial theory in providing suitable solutions to solving the encountered problems and was highly effective in using the appropriate theory to solving the problem, 12.5% did not use the theory effectively. Findings from the students' performances revealed that term project aids students' learning and enables them to connect theory with practice. This assures the compliance of the Bachelor of Applied Environmental Science (BAES) degree program with the original program design and facilitates the success of Mount Royal University's BAES degree students.
Applied Degree Program, Collaborative Project-Based Learning, Connecting Theory with Practice, Environmental Education, Principles of Eco-Industrial Development, Problem-Based Learning, Term Project
To cite this article
Israel Dunmade, An Assessment of Students' Learning from Term Projects, Science Journal of Education. Vol. 1, No. 5, 2013, pp. 97-103. doi: 10.11648/j.sjedu.20130105.17
L. Helle, P. Tynjälä and E. Olkinuora, Project-Based Learning in Post-Secondary Education: Theory, Practice and Rubber Sling Shots, Higher Education, Vol. 51, No. 2, 287-314.
J.W. Thomas, "A review of research on project-based learning," Online available from http://www.autodesk.com/foundation.
I. Dunmade, Effectiveness of Term Projects in Helping Students to Connect Theory with Practice: an Evaluation of Students' Perspectives, Education Journal, Vol. 2, No. 1, 1-6.
L. Andresen, D. Boud and R. Cohen, Experienced-based learning, In: G, Foley. (Ed). Understanding adult education and training, 2nd ed. Sydney: Allen & Unwin, 2000, 225-239.
M. Daniels and L. Asplund, Full Scale Industrial Project Work: a one semester course, In the proceedings of the 29th ASEE/IEEE Frontiers in Education Conference, November 10 - 13, 1999 San Juan, Puerto Rico.
A. Gitterman, Teaching students to connect theory and practice, Social Works with Groups, Vol. 11, No. 1/2, 33-41.
F. A. Korthagen and J. P. Kessels, Linking theory and practice: Changing the pedagogy of teacher education, Educational Researcher, Vol. 28, No. 4, 4-17.
D.H. Allsopp, D. DeMarie, P. Alvarez-McHatton and E. Donne, Bridging the Gap between Theory and Practice: Connecting Courses with Field Experiences, Teacher Education Quarterly, Winter 2006, 19-35.
American Association for the Advancement of Science (AAAS), Science for all Americans: Project 2061. New York: Oxford University Press, 1990.
Tsang, A.K., In-class Reflective Group Discussion as a Strategy for the Development of Students as Evolving Professionals, International Journal for the Scholarship of Teaching and Learning, Vol. 5, No. 1, 1-20, Online available from http://www.georgiasouthern.edu/ijsotl
C.H. Major and B. Palmer, "Assessing the effectiveness of problem-based learning in higher education: lessons from the literature, Academic Exchange Quarterly," vol. 5(1), Spring 2001. Online available from http://www.rapidintellect.com/AEQweb/mop4spr01.htm
L.K.J. Baartman, T.J. Bastiaens, P.A. Kirschner and C.P.M. Van der Vleuten, "Evaluation assessment quality in competence-based education: A qualitative comparison of two frameworks," Educational Research Review, Vol. 2, 114-129.
J.E. Mills and D.F. Treagust, "Engineering education - is problem-based or project-based learning the answer?" Australasian journal of engineering education"


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Policy Internship (2019)
, , Part-time
Common Sense (https://www.commonsensemedia.org/) is the nation's leading independent nonprofit organization devoted to helping kids and families thrive in a world of media and technology. Every day, millions of families turn to Common Sense for trusted advice and unbiased ratings. We offer the largest library of independent age-based and educational ratings and reviews for everything your kids want to watch, play, read, and learn. Our movie and TV ratings are available on Netflix, Comcast, DirecTV, and many other partner networks, helping families and kids make smart media choices. Through our schools program, we provide educators with a curriculum that teaches the essential digital citizenship and literacy skills every student needs to succeed. More than 100,000 member schools across the country use our ratings and free curriculum and content. We also work with policymakers, industry leaders, legislators, and a grassroots community of concerned parents and teachers to improve the media and education landscape for kids, families, and schools.
Common Sense is seeking an all-star intern who can help our Common Sense Kids Action team over the summer. This is a great opportunity for anyone looking to get practical experience in policy and advocacy work at a rapidly growing, national nonprofit.
LOCATION: San Francisco, CA
- Learn the inner workings of an advocacy department as well as the nuances of formulating policy
- Conduct research related to policy on media and technology, student privacy, and early childhood
- Assist in tracking legislation
- Contribute to our social media and other outreach work
- Assist individual team members with other administrative duties as necessary
- Demonstrated interest in public policy, advocacy, and social justice issues
- Detail-oriented, highly organized, with strong follow-through
- Team player and willing to learn
- Flexible and responsive; able to perform in a dynamic work environment
- Enthusiastic, can-do attitude
- You're a data wonk, but also someone able to tell the bigger story.
- You're hungry for discovery and a constant problem-solver.
- Your unique experience and perspective drive your work.
- Ideal start date: Orientation is Monday, June 17, from 10 a.m.–3 p.m. On-site attendance is preferred.
- Ideal end date: Friday, August 9, 2019
- Hours: ~15-20 hours/week
- Interns need to be available to attend a minimum of six weekly educational Lunch & Learns (there will be seven total). Lunch & Learns will be every Wednesday from 12 p.m.–1 p.m. (PST). On-site attendance is preferred.
- We will work with your clinical or internship office for school credit if necessary.
- This is a paid opportunity.
Common Sense values the diversity of its employees.


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Beautiful Best Time Of Year to Tent for Termites
– Nowadays, ridge tents have moved with the occasions and make the most of light-weight aluminum sticks and outer fly sheets produced from waterproof nylon or polyester. Up to date ridge tents are often finest suited to 2 individuals. Due to this fact, if you happen to're involved in one thing larger, bell tents are probably the closest you may get to some giant classic ridge tent. best time of year to tent for termites
how to prepare for tent fumigation of your home from Information Best Time Of Year to Tent for Termites, source:thespruce.com
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It's not that gold is becoming a great deal of more expensive. It's which the forex in which gold prices are quoted has depreciated a great deal. (The U.S. dollar has dropped virtually ninety seven% of its benefit Because the Federal Reserve was designed in 1913.)
The most beneficial prices for gold and silver are those that are most reliable. Monex gold and silver prices are an marketplace highly regarded reference for many years, with more than $55 billion in precise consumer physical steel transactions. After some time, gold and silver prices are likely to move in concert, typically in opposition of depreciating fiat currencies. Just lately, the cost of gold has long been about 60 instances the price of silver, though historically it had been closer to twenty times.
This week's choice from the Swiss National Bank to enable the Swiss franc to float freely has despatched shock waves internationally's marketplaces.
Some bulls hope that this signals that China could reposition a lot more of its holdings into gold, in step with other central banking companies. Chinese buyers started pursuing financial investment in gold as a substitute to financial investment while in the Euro soon after the start of the Eurozone disaster in 2011. China has because turn into the earth's top rated gold customer as of 2013[update].[24]
Treasured metals investments have often been the goal of counterfitters wanting to make A fast buck. To struggle this problem, lots of mints put into action special markers and counterfeit-proof steps, like Sunshine Minting's Mint Mark SI™ element plus the micro-graving carried out with the Royal Canadian Mint.
conditions gold prices today aren't Significantly distinctive from whatever they had been whenever they had been past quoted at $twenty an ounce.
S. marketplaces are shut. But the largest and many influential marketplace for metals prices could be the U.S. COMEX exchange. The quotation for quick settlement at any offered time is properly the place price tag.
A gorgeous value to buy or market bullion, which isn't out there in volume, isn't attractive. Such as, Monex Deposit Firm has executed more than fifty five billion bucks in Actual physical client valuable metals transactions.
So, if you will find been a drop in gold worth, why are we recommending you invest in it? We have got three explanation why You do not require to bother with the drop in gold price.
The major gold mining inventory index missing virtually 30% of its price. Gold is actually a more info tangible form of money; mining stocks are fiscal assets which can be subject matter to a host of further hazards In addition to the marketplace price of gold.
Inflation may be the change in the U.S. dollar's purchasing ability, that's frequently measured by the core Consumer Selling price Index (CPI). This is the evaluate of the prices connected to a basket of services, excluding explosively priced objects like food and Strength.
Lots of people in existence recognize the real splendor of a wonderfully minted gold coin. Take the cash from the well-known American Gold Eagle system for instance, with obverses that includes Weinman's wonderful Strolling Liberty along with the reverses depicting Mercanti's rendition of a Bald Eagle in addition to a shield, a symbol of American power and pride.
" Buying and selling takes place to the spot current market Along with the long run market place employed for commodities. Gold will not be reliant on only one central lender or government. Because of this, important metal investments are used to hedge towards inflation and economical turmoil.
Purchasing gold has been around quite a long time, and there's a reason behind that. The gold price commonly follows an upward pattern. And, even though other investments are executing improperly, gold will stay important.
The 2-Minute Rule for gold prices
It's not that gold is becoming a great deal of more expensive. It's which the forex in which gold prices are quoted has depreciated a great deal. (The U.S. dollar has dropped virtually ninety seven% of its benefit Because the Federal Reserve was designed in 1913.)


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Welcome to Redialled.com
Clean and Clear information
Clean and Clear information
What do we understand by natural body building supplements? Natural bodybuilding supplements are nothing but food supplements derived through available natural resources. These body building supplements augment your diet and exercise to help build lean mass and strengthen muscles. With the combination of the right diet and the right kind of supplements, many sports persons or body builders successfully build up muscle and lean mass and provide energy. The various forms of natural body building supplements are protein powders, whey powders, mega amino acids arginine, creatine, calcium, folic acid and fibers.
Since these are all naturally occurring products, they are all considered completely legal and safe to use without the possibility of any side effects such as renal stress, kidney problems or diarrhea. There are no proven studies to prove these side effects occur except diarrhea which happens only if more than the prescribed dosage is consumed. Once the dosage is adjusted to normal, diarrhea is taken care of immediately. However over usage of any kind of supplement can prove detrimental, if taken without supervision. So if you are an exercise freak or love being active in sports and you want to follow a regime including exercise and dietary supplements, do so under the supervision of a coach, a physician or under a carefully carved out supplement plan.
To understand which supplement is good for you it also becomes important to understand what each kind of supplement helps with. Protein Supplements may be of various varieties including creatine, protein powder and testosterone supplements. Creatine is naturally found in most meat products and is very effective for building of lean mass and providing the body with energy. Protein powder is available in the form of flavored milkshake, pudding, bars and gels. Testosterone supplements affect the hormonal balance in the body stimulating male hormones leading to increase in muscle mass. Before you try these body building supplements, it is good to be educated about them.


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Meet our school's warm, friendly and knowledgeable faculty and staff:
Administrative Staff
Lauren Denton, Director
Originally from New Jersey, Lauren moved to Sequim in August of 2014 after living in Seattle for 10 years. She was a double major in communications and Spanish at Villanova University and has a master's degree in public administration with a focus on nonprofit management from the University of Washington. In between undergraduate and graduate school Lauren spent one year working as a student coordinator at a language school in Costa Rica and two years serving as a Peace Corp volunteer in HondurasAfter completing her master's degree, Lauren worked for eight years as the Director of Global Programs at University Preparatory Academy, an independent middle/high school in Seattle. Lauren and her husband, Keith, have two children at Five Acre School – Sawyer and Marea. Lauren's passion for child-centered learning and becoming a part of the Five Acre community were priorities for the Dentons when they decided to move to the Olympic Peninsula. Her interests include running, hiking, camping, theater and global education.
Amber Tjemsland, Office Manager
Amber has been an active member of our community since her children began at Five Acre. Prior to becoming a mother, Amber worked as a credentialed Art and Ceramics teacher in California. In addition to her role on the Five Acre PSO, Amber taught in the Discovery class on Wednesdays for two years, was a substitute teacher and she has also served regularly as an office substitute. Given Amber's familiarity with our families, school culture, and teaching philosophy, we feel she is great fit for this position. Amber plans to become a long term member of our office staff and we will be training Amber to take on the role of Business Manager in the future.
Autumn Piontek-Walsh, Owner
Primary Class (Pre-K)
Tessa Kirner
Jordan Schiefen
Kylee Jeffers
Terry Wilson
Terry teaches in the Primary Classroom. She received her Bachelor's degree in Biology with a Chemistry minor from California State University Sacramento in 1986. She worked in the analytical laboratory and Environmental Chemistry field for over 20 years in California. While working she spent as much time as she could volunteering at her first daughter Sarah's Waldorf school. This is where she found a love of teaching and working with children. Ready for change, she and her husband Scott moved the family to the Olympic Peninsula in 2005 and she began home schooling with her daughter 5th through 9th grade. During this time her second daughter Olivia was born. Olivia attended Five Acre School through the 5th grade. While Olivia was at Five Acre, Terry began to volunteer in her classroom which lead to substitute teaching in all the classrooms. In 2013 she took a position in the Primary Classroom and has been loving it ever since.
Discovery Class (grades K-1)
Andrea Olson
Andrea has been an early childhood educator at Five Acre school for many years. She loves animals and nature which translates to the way she works with children. Andrea is gentle and calm, always trusting that children will lead her in her best teaching.
Kristine Bagley
Miriame cherbib, also Foreign Language (French)
Miriame was born in Paris, France. After getting a Master degree in International Economics and working in a research institute on Energy, Climate Change and Development, she moved to Sequim in 2014 and completed a year long course on the art of teaching French with the "Alliance Française". She is now a certified French Teacher. She joined Five Acre school in 2016. She also teaches French culture and history at the Continuing Education at Peninsula College and gives private French language classes for all ages.
Adventure Class (Grades 2-3)
Sarah Bones
Sarah was born in Montana and grew up in Montana, Alabama and also lived in Alaska. She moved to the Washington in 2005 to be with her mother and sisters. When Sarah started her family, she sought out a school for her boys that would fit their active lifestyle and provide an active learning environment. She has been a parent at Five Acre since 2010. In 2011 Sarah decided to pursue her teaching degree. She spent several months' student-teaching in the Explorer classroom. In the spring of 2013 Sarah earned her Bachelor's degree in Elementary Education with an endorsement in Reading from City University. She was hired to teach at Five Acre in the fall of 2013.
Sarah has two children and lives on a small farm in the Agnew area. The Farm has two dairy cows, a horse, two dogs, three cats, rabbits, chickens, peacocks, fish and a bearded dragon. She enjoys reading, being outside, hiking, walks with her boys, gardening, crocheting and making homemade lip balms and salves. She loves living in the Pacific Northwest! Sarah loves teaching and loves how much she learns FROM the students. Laughing with the students is one of her favorite parts of her job.
Explorer Class (Grades 4-6)
Tom Harris
Tom Harris has been teaching the upper elementary students at Five Acre School since he was hired in 2001. Tom earned his bachelor's degree from Brown University in 1986, got his teaching degree from the Upper Valley Teacher's Institute, and was awarded a Master's degree from the University of Vermont in 1992.
Before coming to Five Acre School Tom taught in both Vermont and California. While still in college Tom started working as a summer seasonal backcountry ranger in Olympic National Park. He worked in Olympic National Park for ten seasons from 1985 through the summer of 1994.
Tom is married to Kate Reavey and has two children, Maeve and William, both of whom graduated from Five Acre School and attend the Sequim public schools. Tom is a long time soccer player, coach and organizer. He also enjoys fly fishing, hiking and camping. Tom also had a short career as a timber framer which grew out of his long time interest in woodworking.
Music and Performing Arts
Rosie Sharpe
Rosie Sharpe has worked at Five Acre School since 2008. Rosie has a degree in both Art and English Literature from the University of Washington, and also attended Penland School of Crafts for two years. She has completed Levels one and two Orff Schulewerk Certification (an innovative approach to teaching music) and plays Fiddle in a Contra Dance Band.
Previous adventures include cooking on various boats in Alaska, designing textiles for industry, teaching yoga, and working at Children's Montessori in Port Angeles. She has two children attending the school. She loves to quilt, read, hike, garden and spend time with her family including her eight brothers and sisters.


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No. 06-4402-ag.United States Court of Appeals, Second Circuit.
August 1, 2007.
Page 639
UPON DUE CONSIDERATION of this petition for review of a decision of the Board of Immigration Appeals ("BIA"), it is hereby ORDERED, ADJUDGED, AND DECREED, that the petition for review is DENIED in part and DISMISSED in part.
Lin Li, New York, NY, for Petitioner.
Chuck Rosenberg, United States Attorney for the Eastern District of Virginia, Olivia Hussey, Assistant United States Attorney, Alexandria, VA, for Respondent.
PRESENT: Hon. CHESTER J. STRAUB, Hon. ROSEMARY S. POOLER, Hon. ROBERT A. KATZMANN, Circuit Judges.
Petitioner Zi Yan Lin, a native and citizen of China, seeks review of an August 31, 2006 order of the BIA affirming the April 19, 2005 decision of Immigration Judge ("IJ") Gabriel C. Videla denying petitioner's applications for withholding of removal and relief under the Convention Against Torture ("CAT") See In re Zi Yan Lin, No. A78-852-668 (B.I.A. Aug. 31, 2006), aff'g No. A78-852-668 (Immig. Ct. N.Y. City Apr. 19, 2005). We assume the parties' familiarity with the underlying facts and procedural history of the case.
When — as in Lin's case — the BIA adopts the decision of the IJ and supplements the IJ's decision, this Court reviews the decision of the IJ as supplemented by the BIA See Yan Chen v. Gonzales, 417 F.3d 268, 271 (2d Cir. 2005). This Court reviews questions of law and the application of law to undisputed fact de novo. Secaida-Rosales v. INS, 331 F.3d 297, 307 (2d Cir. 2003). However, we review factual findings under the substantial evidence standard, treating them as "conclusive unless any reasonable adjudicator would be compelled to conclude to the contrary." 8 U.S.C. § 1252(b)(4)(B); see Zhou Yun Zhang v. INS, 386 F.3d 66, 73 n. 7 (2d Cir. 2004).
As an initial matter, because Lin failed to raise to the BIA any challenge to the IJ's finding that she did not qualify for withholding of removal under INA § 241(b)(3), we lack jurisdiction to consider such a challenge and dismiss the petition for review to that extent. See 8 U.S.C. § 1252(d)(1); Karaj v. Gonzales, 462 F.3d 113, 119 (2d Cir. 2006). For similar reasons, we decline to consider Lin's due process claim regarding the withdrawal of her asylum application. See Theodoropoulos v. INS, 358 F.3d 162, 172-73 (2d Cir. 2004) (noting that where the BIA may decide underlying issues of fairness of process, and could have provided the possibility of relief, petitioner was required to exhaust even due process claims before petitioning the Court for review). Additionally, in her appeal to the BIA, Lin claimed that, because she had left China illegally, it was more likely than not that she would be tortured if she returned to that country. At no point in her appeal did Lin claim that her fear stemmed from
Page 640
her practice of Falun Gong. Thus, Lin failed to exhaust any claim based upon such practice. See Lin Zhang v. U.S. Dep't of Justice, 480 F.3d 104, 123-24 (2d Cir. 2007) (holding that although issue exhaustion is not jurisdictional, as a general rule, this Court will not consider issues not raised to the BIA). Although we may decline to consider unexhausted issues regardless of whether the Government urges us to do so, we note that, in this case, the Government has affirmatively asserted that Lin failed to exhaust her Falun Gong claim. See id.
Turning to Lin's remaining claim, substantial evidence supports the agency's determination that Lin failed to establish eligibility for relief under the CAT. "To establish eligibility for protection under the CAT, an applicant bears the burden of proving that `it is more likely than not that he or she would be tortured if removed to the proposed country of removal.'" Mu Xiang Lin v. U.S. Dep't of Justice, 432 F.3d 156, 159 (2d Cir. 2005) (quoting 8 C.F.R. § 1208.16(c)(2)).
Although some of the documentary evidence in the record indicates that some prisoners in China have been tortured, Lin has not established that someone in her particular alleged circumstances, i.e., someone who left China illegally, is more likely than not to be tortured if forced to return to China See Mu-Xing Wang v. Ashcroft, 320 F.3d 130, 144 (2d Cir. 2003). Thus, the IJ's denial of Lin's application for CAT relief is supported by substantial evidence.
For the foregoing reasons, the petition for review is DENIED in part and DISMISSED in part. The pending motion for a stay of removal in this petition is DENIED as moot.