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This beautifully positioned home situated on 1.5 hectares of land
and taking in the most amazing views of the Tamar is the ideal property for those who are wanting to experience a lifestyle change.
The land is suitable for alpacas, horse, pony or even some sheep.
Only a 40 minute drive to Launceston and with the choice of schools in the area this property will open your eyes to the choices it has to offer you and your family.
2 living areas both with wood heating
Spacious myrtle wooden kitchen and separate dining area
3 bedrooms all with built-ins and the main with a walk-in robe and an ensuite
Tidy family bathroom
Heat pump
Spacious rumpus or games area
Externally you have a double carport and a large work shed
The property is on town water and
has 20 solar panels
Undercover outside entertaining areas
$335 per week
Available 17th August, 2018


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Alleged Moto G4 photos leak out, fingerprint scanner apparently included
posted by Florin T. / Apr 18, 2016, 12:47 PM
Motorola will probably release both a regular Moto G4, and a Moto G4 Plus, though for now we don't know what's going to differentiate the two handsets (we can, of course, assume that the Plus model will be larger).
The Moto G4 and Moto G4 Plus could be announced in the near future alongside new Moto X and Moto E phones. As you may know, Motorola didn't launch any handsets this year, so it's about time for the company to show us something new, isn't it?
source: Weibo (in Chinese) via HelloMotoHK, Nowhereelse
24 Comments
1. abhishekvashishth
Posts: 20; Member since: Aug 19, 2014
Ugly af. Thanks to Lenovo
posted on Apr 18, 2016, 12:52 PM 25
9. zeeBomb
Posts: 2318; Member since: Aug 14, 2014
DAT front facing fingerprint...will be accurate. But the overall design is pretty boring as f...it seems like it got inspiration from the Lenovo Vibe Series
posted on Apr 18, 2016, 2:15 PM 2
21. Rifqiandi
Posts: 23; Member since: May 28, 2015
Go learn some fact. IT IS MOTOROLA WHO DESIGN LENOVO PHONE, AND THEIR MOTO. The company said in a statement, "MBG will continue to drive Lenovo's overall mobile business, but will now rely on Motorola to design, develop and manufacture smartphone products." "Motorola to DESIGN" thenextweb.com/mobile/2015/08/27/motorola-will-now-design-and-build-lenovo-phones/#gref Oh wow. NOW WHO'S BAD AT DESIGNING? Motorola? Lenovo USE MOTOROLA'S DESIGN. Yes... ITS MOTOROLA.. Not Lenovo, people. *Facepalm
posted on Apr 19, 2016, 12:13 AM 1
6. NoAllegiance unregistered
Should not cost more than $150, why pay more when the name Motorola is not worth $179 anymore. Number 1 - uses mediatek Number 2 - front speaker is gone Number 3 - possible updates may be reduced under lenovo's supervision. Number 4 - Lenovo Vibe K5 and K5 Plus is around $150.
posted on Apr 18, 2016, 2:01 PM 1
26. NoAllegiance unregistered
Edit, Mediatek may be the E only
posted on Apr 25, 2016, 9:16 AM 0
7. SupermanayrB
Posts: 1188; Member since: Mar 20, 2012
Been done with Moto since the DROID Maxx. Note 3, Note 4 & soon to be in the Note 6 when it drops.
posted on Apr 18, 2016, 2:01 PM 1
10. marorun
Posts: 5029; Member since: Mar 30, 2015
Love how much hate some ppl can have :)
posted on Apr 18, 2016, 2:19 PM 1
15. ibend
Posts: 6747; Member since: Sep 30, 2014
they just hate lenovo... if moto didnt aquired by lenovo and release MotoG with exact same design with above, people will love it, lol
posted on Apr 18, 2016, 7:31 PM 2
13. bossman
Posts: 264; Member since: Jan 27, 2016
This is not the Moto flagship, it could just be the Moto G shipped with a fingerprint sensor. Many other companies have shipped their lower end phones with fingerprint sensors before. Also look at the material. It is plastic with out any metal on the side. It is just their middle or lower end product.
posted on Apr 18, 2016, 3:33 PM 0
19. Joshing4fun
Posts: 1244; Member since: Aug 13, 2010
Only thing I care about with this phone is price. It needs to be $299 or less to even matter in this insanely competitive market.
posted on Apr 18, 2016, 10:09 PM 0
24. boys.noize
Posts: 19; Member since: Jan 03, 2016
please base the design on last year's pretty moto G
posted on Apr 19, 2016, 7:32 AM 0
25. aleant36
Posts: 71; Member since: Feb 03, 2015
Never understood why all phones have to have a cutout for fingerprint scanner. Couldn't they just make it flush with the chin of the phone since most use glass? And maybe a print logo indicating where to put your finger.
posted on Apr 19, 2016, 3:39 PM 0
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subset: commoncrawl


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The Single Best Strategy To Use For keto ultra diet reviews
Advised you, This may be difficult. Junk food stuff consumption really should be stopped permanently and sugar can get replaced by stevia.
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Keto Ultra Diet doesn't have an impact on muscles. In reality, it works by using the Body fat cells hooked up towards the muscles and offers them a area for improvement, thus increasing the lean muscle mass.
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The Elephant to Hollywood by Michael Caine
Michael Caine's second work of memoir brims with his gift for genial anecdote, but this time there's a hint of sadness as he looks back
"This is not a serious tome by a pompous old actor," Michael Caine reassures us at the start of this, his second autobiography. Indeed it is not. The 150,000 or so UK fans who bought his equally endearing memoir What's It All About? in 1992 (the innocent days before supermarkets sold books, so a phenomenal number) may well recognise some of the stories in The Elephant to Hollywood, but could scarcely grumble on hearing them again. Rarely has a raconteur sustained such a level, unfussy tone.
Born in 1933 with a mild, incurable eye disease that makes the lids swell, and raised in a loving, poor family in the Elephant and Castle, the young Caine began working in steelyards, before moving on, via a youth-club…


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Peterborough United are likely to need 25 points from their final 15 League One matches to secure a place in the League One play-offs.
The average points for the team finishing sixth in League One - the final play-off spot - in the last 10 years is 74. Posh are stuck on 49 points following their disappointing 1-0 home defeat to Plymouth on Saturday (February 2).
There seems little chance of Posh catching top four Luton, Barnsley, Portsmouth and Sunderland, but fifth-placed Charlton have upset their fanbase by seling star striker Karlan Grant on deadline day for £2 million and not funding an equally talented forward with the proceeds.
Posh can't be ruled out as long as they discover how to beat the weaker teams. They have already played Luton, Barnsley and Charlton twice.
The most difficult remaining games appear to be at Ferguson's former club Doncaster (on his 47th birthday) this Saturday (February 9), at home to Sunderland (Easter Monday) and away to Portsmouth (March 30).
When Ferguson took over at Posh in January, 2011 they had 36 points from 22 games. They finished with 79 points from 46 matches.
Anyway the PT has plotted a path to the play-offs
Current points: 49
Points required (based on average points of team finishing 6th in last 10 League One seasons): 74
Feb 9 v Doncaster (away) D
McCann v Fergie to finish all square
Feb 16 v Oxford (away) L
Posh never beat Oxford
Feb 23 v Shrewsbury (home) W
Everyone has started to beat the Shrews
Mar 2 v Wycombe (home) W
Wycombe fluked a win against Posh at Adams Park
Mar 9 v Bradford C (away) D
The Bantams are fighting hard against the drop
Mar 12 v AFC Wimbledon (away) W
The Dons are doomed
Mar 16 v Coventry (home) W
Coventry aren't very good.
Mar 23 v Southend (home) L
Southend wil be stronger when they reduce that injury list
Mar 30 v Portsmouth (away) L
Pompey had a good transfer window
April 6 v Gillingham (home) W
Not even Gaby Zakuani can save the Gills
April 13 v Blackpool (away) D
Pool have over-achieved again
April 19 v Fleetwood (away) D
Could be a big game on Good Friday
April 22 v Sunderland (home) L
Sunderland also enjoyed a good transfer window
April 27 v Walsall (away) W
Walsall could be relegated by the time Posh get there
May 4 v Burton (home) W
Burton will have nothing to play for on the final day
Total points: 74


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Dating a rawlings jersey Live and free no sing up sex chats
Atlantic City owed Borgata 5 million in tax refunds after years of successful tax appeals by the casino, according to the state.The settlement is projected to save the city million in potential debt—savings which amount to a 22 percent reduction of the city's 4 million total debt, according to Moody's, albeit, as Moody's noted: "[W]hile it does not solve the city's problems, the settlement makes addressing those problems considerably more likely." The city will bond for the million through New Jersey's state Municipal Qualified Bond Act, making it a double whammy: because the bonds will be issued via the state MQBA, they will carry an A3 rating, ergo at a much better rate than under the city's Caa3 junk bond status.Or, as Genevieve Nolan, a vice president and senior analyst at Moody's Investors Service, notes: "With their cases focusing on not just a government's ability to pay its debts, but also an ability to provide basic services to residents, Stockton and Detroit opened a path for future municipal bankruptcies."Mr.Pfeiffer notes that East Cleveland, Ohio, was the first city to invoke service insolvency after Detroit.Rosselló added that these figures are for government debt proper—not the debt of issuers of the public corporations (excepting the Highways and Transportation Authority), Puerto Rico's 88 municipalities, or the territory's handful of other semi-autonomous authorities, and that its provisions do not count on Congress to restore Affordable Care Act funding. Rosselló said he plans to determine the amount of debt the Commonwealth will pay by first determining the sums needed for (related to what Mr.Pfeiffer raised above] "essential services and contingency reserves." The Governor noted that Puerto Rico's debt burden will be based on net cash available, and that, if possible, he hopes to be able to use a consensual process under Title VI of PROMESA to decide on the new debt service schedules.
Ergo, ACA is taking another swing at the city: it is seeking: Claiming the municipality "fraudulently induced" ACA to enter into the transaction by representing that the city had authority to enter the contracts.
In this a.m.'s e Blog, we consider the ongoing challenges for the historic municipality of Petersburg, Virginia as it seeks to depart from insolvency; we consider, anew, the issues related to "service insolvency," especially assisted by the exceptional insights of Marc Pfeiffer at Rutgers, then turning to the new fiscal plan by the Puerto Rico Fiscal Agency and Financial Advisory Authority, before racing back to Virginia for a swing on insolvent links.
For readers who missed it, we commend the e Blog earlier this week in which we admired the recent wisdom on fiscal disparities by the ever remarkable Bo Zhao of the Federal Reserve Bank of Boston with regard to municipal fiscal disparities.
The Governor notes the Fiscal Plan is intended to achieve its objectives through fiscal reform measures, strategic reform initiatives, and financial control reforms, including fiscal reform measures that would reduce Puerto Rico's decade-long financing gap by .3 billion through: The new Fiscal Plan marks an effort to achieve fiscal solvency and long-term economic growth and to comply with the 14 statutory requirements established by Congress' PROMESA legislation, as well as the five principles established by the PROMESA Oversight Board, and intended to sets a fiscal path to making available to the public and creditor constituents financial information which has been long overdue, noting that upon the Oversight Board's certification of those fiscal plans it deems to be compliant with PROMESA, the Puerto Rico government and its advisors will promptly convene meetings with organized bondholder groups, insurers, union, local interest business groups, public advocacy groups and municipality representative leaders to discuss and answer all pertinent questions concerning the fiscal plan and to provide additional and necessary momentum as appropriate, noting the intention and preference of the government is to conduct "good-faith" negotiations with creditors to achieve restructuring "voluntary agreements" in the manner and method provided for under the provisions of Title VI of PROMESA.
Related to the service insolvency issues we discussed [above] this early, snowy a.m., Gov.


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Frizzled (Fz) signaling regulates the establishment of planar cell polarity (PCP). The PCP genes prickle (pk) and strabismus (stbm) are thought to antagonize Fz signaling. We show that they act in the same cell, R4, adjacent to that in which the Fz/PCP pathway is required in the Drosophila eye. We demonstrate that Stbm and Pk interact physically and that Stbm recruits Pk to the cell membrane. Through this interaction, Pk affects Stbm membrane localization and can cause clustering of Stbm. Pk is also known to interact with Dsh and is thought to antagonize Dsh by affecting its membrane localization. Thus our data suggest that the Stbm/Pk complex modulates Fz/Dsh activity, resulting in a symmetry‐breaking step during polarity signaling.
In multicellular organisms epithelia are polarized not only in the apical–basal axis, but also within the epithelial plane. In vertebrates, this epithelial planar cell polarization (PCP) is evident in the regular appearance of fish scales and bird feathers, and in internal organs such as the inner ear and the oviduct where hairs and cilia are oriented precisely with respect to each other (Eaton, 1997; Lewis and Davies, 2002; Mlodzik, 2002). In addition, the process of convergent extension (CE) during vertebrate gastrulation and neurulation shares many features and components of PCP signaling (reviewed in Myers et al., 2002; Wallingford et al., 2002a).
In Drosophila melanogaster, PCP is manifest in diverse structures: the hairs of wing cells all point distally, and the bristles of the thorax and the abdomen all grow in a posterior direction (Adler and Lee, 2001; Adler, 2002). In addition, the 20 cells of each ommatidium of the fly's facet eye are precisely arranged with respect to each other and to the dorsoventral and anteroposterior axes. Genetic studies have identified several genes involved in the establishment of PCP. Central to these polarity genes is the Frizzled receptor (Fz) and the non‐canonical Fz/PCP signaling pathway (Shulman et al., 1998; Adler, 2002; Mlodzik, 2002). It is thought that an unknown ligand leads to a Fz activity gradient. The signal is then transduced via the adapter protein Dishevelled (Theisen et al., 1994; Axelrod et al., 1998; Boutros et al., 1998) and small GTPases (Strutt et al., 1997; Fanto et al., 2000) to a JNK MAP kinase module, generating a transcriptional response in the eye (Paricio et al., 1999; Weber et al., 2000). In the wing and eye, the pathway leads to cytoskeletal changes involving the Rho associated kinase (Drok; Winter et al., 2001). Several additional genes such as flamingo (fmi; also known as starry‐night), diego (dgo), strabismus (stbm; also known as van‐gogh), fat, dachsous and prickle (pk) are required for PCP signaling (Heitzler et al., 1993; Adler et al., 1998; Taylor et al., 1998; Wolff and Rubin, 1998; Chae et al., 1999; Usui et al., 1999; Feiguin et al., 2001; Rawls et al., 2002; Yang et al., 2002). While the relationship of most of these genes with the Fz/PCP pathway is unclear, the atypical cadherin fmi can stimulate and repress Fz activity at different time points during PCP establishment (Usui et al., 1999; Das et al., 2002).
In the eye, PCP signaling specifies the R3 and R4 photoreceptor cell fates and is reflected by the mirror image arrangement of ommatidia (facets) with respect to the dorsoventral midline (equator; see also Figure 1A, B and C). This arrangement is generated by a 90° rotation of the developing ommatidial preclusters. In addition, a loss of symmetry between R3 and R4 leads to opposing chiral arrangements dorsally and ventrally of the equator (Figure 1A and B; for reviews see Wolff and Ready, 1993; Mlodzik, 1999; Reifegerste and Moses, 1999). The crucial signaling events occur as five‐cell preclusters, consisting of the R8, R3/4 and R2/5 precursors, leave the morphogenetic furrow (Zheng et al., 1995). Higher Fz activity in the R3 relative to the R4 precursor leads to stronger activation of the JNK cascade and thus higher Delta (Dl) transcription in the R3 precursor. Dl then activates Notch (N) in the R4 precursor (Cooper and Bray, 1999; Fanto and Mlodzik, 1999; Tomlinson and Struhl, 1999). This results in increased Fmi levels and consequently repression of Dl expression (Das et al., 2002), stabilizing the R3/R4 fate decision.
PCP genes can be subdivided into two groups by their genetic requirement in the R3 or R4 precursor cells. fz, dsh and Dl are required in the R3 precursor cell (Zheng et al., 1995; Fanto and Mlodzik, 1999; Tomlinson and Struhl, 1999), while stbm and N function in R4 (Wolff and Rubin, 1998; Tomlinson and Struhl, 1999). fmi is the only gene required in both cells (Das et al., 2002). Loss‐of‐function (LOF) and gain‐of‐function (GOF) alleles of core planar polarity genes lead to ommatidia that adopt a chirality and rotate, but the acquisition of the R3/R4 fate and associated chirality occurs stochastically, and the direction and extent of rotation is random. In addition, ommatidia can become symmetric.
The function of the core polarity gene pk in the eye and its place with respect to other PCP genes is largely unknown. pk encodes three protein isoforms (Pk, PkM and Sple) differing at the N‐termini (Gubb et al., 1999). The common part contains a PET domain, a region conserved in Pk, Espinas and mouse Testin (Divecha and Charleston, 1995; Gubb et al., 1999), followed by three LIM domains. pk null mutations (pkpk–sple class) affect PCP in all tissues. pksple alleles affect only the Sple isoform and show planar polarity defects in the eye and leg. The pkpk class of alleles, which affects only the Pk isoform, shows aberrant wing hair and thorax bristle patterns (Gubb et al., 1999). The PkM isoform is not expressed during PCP signaling and has no known function. At the molecular level, the PET/LIM domain region of Pk can interact with the DEP domain and C‐terminus of Dsh in vitro (Tree et al., 2002). It has been suggested that Pk could prevent the Fz‐dependent membrane recruitment of Dsh, thereby antagonizing Fz pathway activity (Tree et al., 2002).
In order to place pk more precisely within the PCP gene network, we used mosaic analysis and tested genetic and molecular interactions of Pk with candidate PCP genes. In contrast with previous reports (Zheng et al., 1995; Gubb, 1998), we find that pk function is not required in R3 but is required in the R4 precursor. We show that pk and stbm interact with one another genetically and physically. Pk also affects convergent extension during gastrulation in Xenopus, and Pk and Stbm mutually influence their subcellular localization in animal cap cells. Our data indicate that Stbm recruits Pk to subdomains of the plasma membrane through a direct interaction. We propose that this recruitment leads to the assembly of a Stbm/Pk‐containing signaling complex that alters Fz/Dsh activity and localization, thus breaking planar polarity signaling symmetry.
pk is required in the R4 precursor for PCP establishment in the eye
Null alleles for pk (pkpk–sple13) display a typical PCP eye phenotype: misrotations and random chirality with an associated loss of the mirror symmetry line, the equator, at the dorsoventral midline (Figure 1C and D; see also Zheng et al., 1995; Gubb et al., 1999). The isoform‐specific alleles pksple1 and pkpk1 show either a weaker phenotype or no phenotype, respectively (not shown). Thus, unlike in the wing and thorax, the complete loss of pk in the eye is more severe than the loss of either isoform alone (cf. Gubb, 1998; Gubb et al., 1999). These defects occur early in the larva because PCP phenotypes can be detected from their earliest appreciable stage (Zheng et al., 1995; Gubb et al., 1999).
Although previous work has suggested that pk is required in the R3 precursor or in several photoreceptors (Zheng et al., 1995; Gubb, 1998), we find that Pk is necessary only in the R4 precursor for normal PCP signaling to occur (Figure 1E, F and G). We performed mosaic analysis for two independent pk genotypes pknull (pkpk–sple13; Figure 1E) and pksple1 (Figure 1F) and combined this with a careful statistical analysis using a logistic regression model (see below). For both genotypes, ommatidia with a wild‐type R4 precursor adopted the correct chirality, whereas ommatidia with a flipped chirality rarely contained a pk+ R4 precursor (Figure 1E and F; see Figure 1B for scheme; note that photoreceptor identities were assigned according to their respective precursor identity).
The only other wild‐type cell that was apparently under‐represented in flipped ommatidia is the R5 precursor (Figure 1E and F). We used the logistic regression model (Hosmer and Lemeshow, 1989) to assess statistically whether R5 is really required. This model takes into account that R5 has a higher probability of being of the same genotype as R4 (Tomlinson and Struhl, 1999). This analysis demonstrated that R5 is not required. Thus our data showed that only the R4 precursor genotype of pk correlates with the chirality decision (p < 0.001).
To confirm this result we performed a mosaic analysis with a pk rescue construct (sev‐PkSple) in a pk null background. At the time of the R3/R4 fate decision, the sevenless (sev) enhancer drives gene expression in the R3/R4 precursor pair, but not the other R cells (Basler et al., 1991). The sev‐PkSple transgene fully rescues the pknull and pksple1 eye phenotype (not shown). Ommatidia mosaic for sev‐PkSple within the R3/R4 pair (the presence of the transgene is autonomously marked by the w+ pigment) were always rescued when the transgene was present in R4. In contrast, in all of the flipped R3/R4 mosaic ommatidia (48 for pknull, 30 for pksple1) the rescue construct was present only in the R3 precursor that acquired the R4 fate (orange arrow in the example shown in Figure 1G). Again, the genotype of the other photoreceptors had no influence on the chirality decision. Thus we conclude that pk is required in R4, where stbm is also required, but not in the R3 precursor, which requires Fz signaling (see Introduction).
The Pk GOF phenotype is dominantly enhanced by stbm and vice versa
Overexpression of either the Pk or Sple isoforms under the control of the sev enhancer (sev‐PkPk and sev‐PkSple) leads to typical PCP defects: symmetric ommatidia and chirality and rotation defects (Figure 2 and Table I). These phenotypes are strongly enhanced by two alleles of stbm. In addition, sev>PkSple is also enhanced by dgo and two alleles of fmi (Figure 2A and Table I). Other genes, notably components of the MAP kinase cascade, Dl and N show no effect, suggesting that the interactions are specific.
Conversely, the number of affected ommatidia due to sev‐Gal4‐driven Stbm overexpression (Figure 2B) can be enhanced by the removal of one copy of pk, fmi and the stronger dgo allele (dgo380; Figure 2B). Similar dominant LOF interactions have also recently been shown between pk, stbm and fmi (Rawls and Wolff, 2003).
The reciprocal genetic interactions between sev>Stbm and sev>PkSple, together with the requirement of both genes in R4, suggest that Pk and Stbm act together in a protein complex that requires a certain stoichiometry to function properly.
Pk and Stbm interact with each other in vitro
Initial yeast two‐hybrid data showed that Pk and Stbm interact physically with each other (A.Jenny, R.S.Darken, P.A. Wilson and M.Mlodzik, unpublished data). GST pull‐down assays were used to confirm the interaction in an independent system and to map the interacting domains more precisely (Figure 3). Using in vitro translated deletion constructs of the region of Stbm C‐terminal to the last predicted transmembrane domain (scheme in Figure 3A), we showed that a Stbm domain of less than 85 amino acids is required to interact with Pk and with Stbm itself (boxed scheme in Figure 3A).
We similarly characterized the interaction site with respect to Pk. C‐terminal to the LIM domains (Pk Cterm); Pk is not highly conserved among Drosophila, Xenopus and human. However, we noted conserved stretches of basic amino acids and serine residues close to the C‐terminus and a conserved C‐terminal farnesylation motif [CAAX motif: KNC(I/T)IS; see alignment in Figure 3C and scheme in Figure 3B (Zhang and Casey, 1996)]. We generated GST fusion proteins of Pk Cterm (cf. scheme in Figure 3B) and truncations thereof (e.g. lacking either the CAAX motif or the CAAX motif and the stretch of basic amino acids). We find that both the Pk–Stbm interaction and the Pk interaction with itself require a stretch of 131 amino acids close to, but not including, the very C‐terminus of Pk.
To test whether the C‐terminal fragments of Pk and Stbm, as well as the 131 amino acid fragment of Pk required for the interaction, show an effect in vivo, we expressed these fragments under the control of sev‐Gal4. Expression of all three fragments leads to misrotated and symmetric ommatidia and chirality defects (Figure 4A, B and C). This suggests that all of these fragments can interfere with endogenous protein complex formation and, taken together with the genetic interactions between pk and stbm (see above), argues for an interference of endogenous Pk–Stbm complexes.
Pk shows a reduced membrane association in stbm clones in the eye disk
Pk is localized in a web‐like membrane‐associated pattern just posterior to the morphogenetic furrow (Figure 5A′). Around ommatidial row 5, Pk shows a 'double‐horseshoe'‐like pattern in R3 and R4 (Figure 5C′ and red arrow heads in Figure 5A′) and is concentrated to the equatorial side of R3 and where the R3 and R4 cell touch each other. In rows 7 and 8, Pk is often enriched in a horseshoe pattern around the R4 precursor (Figure 5D and yellow arrowheads in Figure 5A′). Double‐label studies show that Pk colocalizes with Fmi (Figure 5A″, C and D; Das et al., 2002; Yang et al., 2002) and also with Stbm (Rawls and Wolff, 2003), a prerequisite for a physiological interaction between Stbm and Pk.
Interestingly, in stbmnull clones (Figure 5E) just posterior to the morphogenetic furrow, the web‐like membrane staining of Pk is not seen, suggesting that Stbm is involved in the recruitment of Pk to the plasma membrane. In ommatidia of later developmental stages, Pk staining remains diffuse, but in some photoreceptor clusters one cell of the R3/R4 pair has Pk partly enriched at the cell cortex (compare yellow arrowheads with each other and with red arrowheads in Figure 5E and E′). This remaining membrane association of Pk might be explained by its CAAX farnesylation motif at the C‐terminus (Figure 3C) which could contribute to membrane localization. However, as a version of Pk in which the farnesylation site had been mutated (sev‐PkSple–CtoA) (Figure 4D) still significantly rescues a pknull mutant, farnesylation is unlikely to be essential to recruit Pk to the membrane in vivo. In addition, fmi could also contribute to Pk recruitment, as Tree and coworkers (Tree et al. 2002) have shown a partially reduced membrane staining of Pk in fmi− clones in wing cells.
Pk affects convergent extension in Xenopus and can change Stbm distribution in the cell membrane
During convergent extension (CE) in vertebrates, posterior neuroectodermal and dorsal mesoderm cells acquire a bipolar cell morphology, intercalate and thus extend the body axis (for reviews see Myers et al., 2002; Wallingford et al., 2002a). Mutations in zebrafish stbm (Jessen et al., 2002) or morpholino‐mediated knockout of zebrafish or Xenopus pk and stbm (Darken and Wilson, 2001; Park and Moon, 2002; Takeuchi et al., 2003; Veeman et al., 2003) prevent elongation of the body axis during gastrulation and neurulation without affecting cell fate. Similarly, overexpression of xFz7 and dominant negative versions of Dsh and Rok2 affect cell polarization and convergent extension in Xenopus (Sokol, 1996; Djiane et al., 2000; Wallingford et al., 2000; Marlow et al., 2002). As it has been shown that overexpression of proteins affecting CE results in defects similar to their loss of function phenotypes (Darken et al., 2002; Jessen et al., 2002; Park and Moon, 2002), we tested whether Pk can affect CE. Compared with uninjected embryos, 87% of the embryos injected dorsally with pk mRNA developed with a significantly shorter dorsal axis, resulting in dorsally bent tadpoles (Figure 6A–C; quantification in Figure 6K). This effect is due to affected CE and not to altered cell fates (assessed by RT–PCR analysis; data not shown).
We also injected RNAs coding for the region common to all Pk isoforms and for Pk Cterm (cf. scheme in Figure 3), both of which strongly interfered with CE (Figure 6K). Because the interaction of Pk with Stbm maps to Pk Cterm and a related Xenopus construct had been shown to act in a dominant negative fashion (ΔPET/LIM; Takeuchi et al., 2003), we suggest that this fragment acts like a dominant negative form and interferes with the formation of endogenous protein complexes or their localization and therefore prevents CE.
We then analyzed the subcellular distribution of Pk and Stbm in animal cap explants injected with doses lower than those required to cause phenotypic effects (see Materials and methods). GFP‐Pk is primarily cytoplasmic and not concentrated at the plasma membrane (Figure 6D). GFP‐xStbm is evenly distributed around the plasma membrane similar to endogenous Stbm (Figure 6F; Park and Moon, 2002). Upon coinjection of GFP‐Pk with unmarked xStbm, GFP‐Pk is relocalized to patches at the plasma membrane (Figure 6E). Similarly, GFP‐xStbm clusters to patches in the membrane after coinjection with Pk (Figure 6G; see Supplementary data available at The EMBO Journal Online for a time‐lapse movie). Antibody staining showed that GFP‐xStbm and Pk colocalize in these patches (not shown). This indicates that Pk and Stbm can influence each other's subcellular distribution, reflecting clustering and possibly the formation of multiprotein complexes within the cell.
A GFP‐Pk Cterm containing the domain that interacts with Stbm shows a distribution in the animal cap similar to Pk‐GFP when injected alone (Figure 6H). Upon coinjection of xStbm, GFP‐Pk Cterm is localized partially to the cell periphery. A fusion of the PET/LIM domain to GFP is unaffected by coinjection of xStbm (Figure 6J). Even though the C‐terminus of Pk can be recruited to the membrane in this assay, the Pk Cterm does not induce the redistribution and clustering of Stbm/Pk into the patches seen with full‐length Pk. We assume that other portions of Pk are required for the formation of the large patches, consistent with the idea of larger functional protein complexes.
In the Drosophila eye, PCP signaling specifies the R3 and R4 photoreceptor fates and the direction of ommatidial rotation. We show that pk function is required in the R4 precursor, as opposed to fz PCP signaling in R3, for control of polarity establishment. Stbm, a transmembrane protein also required in R4, interacts genetically and physically with Pk. This interaction is important for the recruitment of Pk to the plasma membrane. In Xenopus animal‐cap explants, Stbm and Pk relocalize each other to subdomains of the membrane. We propose a model of how Pk/Stbm might regulate Fz/Dsh signaling activity.
Stbm and Pk interact physically and are both required in R4
The in vitro molecular interaction between Pk and Stbm and their mutual relocalization in Xenopus animal caps suggest that they form multiprotein complexes. Several pieces of evidence indicate that the physical interaction is physiologically important: (i) correct membrane localization of Pk depends on stbm function because in stbm mutant tissue Pk staining is diffuse and absent (or strongly reduced) at the membrane (Figure 5E); (ii) Pk and Stbm interact genetically by mutually enhancing each other's GOF (this work) and LOF (Rawls and Wolff, 2003) phenotypes in the eye; (iii) pk is necessary for PCP signaling in the R4 precursor (this work), the same cell in which stbm is required (Wolff and Rubin, 1998); (iv) expression of the interacting domains (Figure 4A–C) of Pk or Stbm interferes with polarity establishment. In particular, a subfragment of 131 amino acids of the C‐terminus of Pk, required for the molecular interaction, is sufficient to affect polarity.
Potential function of the Pk/Stbm complex
Both Pk and Stbm act as if they antagonize Fz signaling. First, in zebrafish, Stbm overexpression can prevent Wnt11 from rescuing a wnt11 mutation (Jessen et al., 2002). Secondly, in the Drosophila wing, overexpression of Pk leads to wing hairs pointing towards the source of the overexpressed protein (Tree et al., 2002), behaving like a fz LOF clone (whereas overexpression of Fz leads to hairs pointing away from the Fz source). stbm LOF clones show the opposite behavior to fz LOF clones: wing hairs point away from the mutant patch, consistent with the mutant tissue having a higher Fz‐activity (Taylor et al., 1998).
In the Drosophila eye, evidence that pk acts antagonistically to fz comes from the fact that the Notch‐signaling‐responsive R4‐specific reporter mδ0.5‐lacZ is expressed for a prolonged period in both R3/R4 precursors in a pksple1 mutant (Cooper and Bray, 1999). This is explained if Fz activity in the R4 precursor is increased, resulting in higher levels of Dl there. This in turn leads to N activation and concomitant mδ0.5‐lacZ reporter expression in both cells of the R3/R4 pair. Conversely, in fz and dsh mutant eye disks (where Fz signaling is absent or reduced and thus Dl should not be upregulated) N‐signaling activity and mδ‐lacZ expression is initially reduced in both cells (Cooper and Bray, 1999; Strutt et al., 2002). Fz activity is also antagonized by stbm in the eye. Mosaic analysis of stbm showed that it has the capability to instruct a cell to become R4 as long as the other cell of the R3/R4 pair is mutant for stbm (Wolff and Rubin, 1998). Therefore, in such an all‐or‐nothing situation, Stbm in the R3 precursor can override a positive signal of Fz, resulting in a cell fate switch to R4 fate.
In a wild‐type situation with all PCP components present in both cells, it is crucial that Stbm activity is higher in R4 than in R3 to ensure proper Fz‐signaling regulation. Therefore it is an intriguing possibility that a Pk/Stbm complex in the R4 precursor ensures such higher Stbm activity, and the associated higher Fz repression there is important for a proper R3/R4 cell fate decision (see also below).
How does the Stbm/Pk complex regulate Fz‐signaling activity?
During PCP establishment in the wing, Fz, Dsh, Dgo, Fmi and Pk are initially localized uniformly around the apical circumference of wing cells (Usui et al., 1999; Axelrod, 2001; Feiguin et al., 2001; Shimada et al., 2001; Strutt, 2001). During and after PCP signaling, these proteins relocalize and become differentially enriched: Pk concentrates on the proximal side of the cell, whereas Fz and Dsh become enriched distally. Fmi becomes enriched at both sides.
In the eye, the situation is analogous. During PCP establishment, signaling components at the R3/R4 cell border are relocalized from a uniform to a more restricted pattern. Stbm‐YFP is localized on the R4 but not on the R3 side, and Fz‐GFP ends up on the R3 but not the R4 side (Strutt et al., 2002). The analogy between the R4/R3 and proximal/distal cell borders is corroborated by the genetic requirements in R3 and R4: the distally localized factors Dsh and Fz are required in R3, while proximally localized Pk is required in R4. Fmi is localized on both poles of each wing cell and also required in both cells of the R3 and R4 pair. The function of fmi has been linked to both proposed complexes, the 'Fz/Dsh side' (fmi is required for apical localization of both Fz and Dsh; Das et al., 2002) and the Stbm/Pk complex. In addition to the genetic interactions between fmi and stbm or pk (Figure 2; Table I; Rawls and Wolff, 2003), a reduced membrane staining of Pk in fmi− clones in wing cells has been shown (Tree et al., 2002).
How do these changes in localization occur? Our localization studies in Drosophila and Xenopus suggest that Pk and Stbm influence each other's localization and form clusters in subdomains of the cell membrane (Figure 7A). Interestingly, such Stbm/Pk complexes also affect Fz‐dependent Dsh membrane localization (data not shown). Thus it is an intriguing possibility that the patches we observe in animal cap cells upon coinjection of Stbm with Pk represent the result of a similar, though un polarized, symmetry‐breaking step during PCP signaling.
The PET/LIM domain of Pk can interact with the DEP‐domain/C‐terminus of Dsh (Tree et al., 2002). This interaction has been suggested to prevent Dsh membrane recruitment. Also, the C‐terminus of Stbm can interact with Dsh as long as the PDZ domain is present (Park and Moon, 2002; A.Jenny, R.S.Darken, P.A.Wilson and M.Mlodzik, unpublished data). As our data suggest that Pk regulates the activity and localization of Stbm (Figure 7A), this regulation might promote or stabilize the interactions of Dsh with Stbm and/or Pk, thereby helping to pull Dsh away from a Fz‐signaling complex. The Stbm/Pk complexes could then cause active release of Dsh from the membrane or target it for degradation, resulting in low levels of Dsh (and by inference Fz; Strutt, 2001) at places where Pk and Stbm are enriched (see Figure 7B). Furthermore, in the R3 cell (or distally in the wing) an unknown factor might act to prevent either the formation of the Stbm/Pk complex or its effect on Dsh.
In conclusion, Pk and Stbm form a functional complex during PCP signaling in Drosophila and during convergent extension in Xenopus. Interestingly, in zebrafish, in addition to its function in CE, Stbm is also required for the caudal migration of hindbrain motor neurons (Jessen et al., 2002). This function of Stbm is independent of Dsh and the PCP genes tested so far. It will be interesting to determine whether Stbm and Pk function together in this context as well.
Materials and methods
Fly strains and genetics
Mutant alleles were w1118, pkpk–sple13 (referred to as pknull in this article), pkpk1, pksple1, rhoA72o, dsh1, dshv26, fzR52fzK21, N55E11, DlrevF10bsk170b FRT40, Df(3L)Pbl (rac1−), Df(3L)Emc5 (rac1−), FRT42D jun2, Δ2–3Sb (all described in flybase), fmiE59, fmiE45 (Usui et al., 1999), dgo308, dgo380 (Feiguin et al., 2001), stbmx (unpublished), stbmnull (Wolff and Rubin, 1998) and drok1 (Winter et al., 2001).
FRT42Dpknull and FRT42Dpksple1 were generated as described (Xu and Rubin, 1993). Eye clones were generated by the Flp/FRT technique with eyFlp (Newsome et al., 2000) and marked with P[w+] or arm‐lacZ (Vincent et al., 1994). Transgenic flies were generated by standard P‐element‐mediated transformation (Spradling and Rubin, 1982).
Genetic interactions were tested using the Gal4/UAS system (Brand and Perrimon, 1993) with sev‐Gal4 (a kind gift from K.Basler) driving UAS‐Sple (a kind gift from D.Gubb) and UAS‐Stbm (a kind gift from T.Wolff). The effect of sev‐Gal4>UAS‐PkPk is too strong to examine genetic interactions and thus we used the weaker directly driven sev‐PkPk. Rotation was scored as defective when a cluster showed a deviation of more than 15° compared with neighboring ommatidia.
Mosaic ommatidia were analyzed to determine the genetic requirement of pk. Because mutant ommatidia choose chirality at random and thus can also adopt wild‐type orientation, we scored genotypes of the photoreceptor precursors with wrong chirality. Note that the photoreceptor cells were numbered according to their precursor genotype, i.e. correcting for the cell fate switch (cf. Figure 1B). When the capability of pk to instruct a particular cell fate was assessed, the R3 and R4 reflect the position in the adult ommatidium [polar/anterior (R3) versus equatorial/posterior (R4)] and therefore not the precursor cell fate for ommatidia with wrong chirality. The genotype of the flies used for the sufficiency experiment was yw eyFlp;pknull (cn+)/pknull (cn+);sev‐PkSple/Δ2–3Sb and yw eyFlp;pksple1/pksple1;sev‐PkSple/Δ2–3Sb. Interaction crosses of sev>Gal4, UAS‐PkSple and sev>Gal4, UAS‐Stbm/allele of interest were grown at 29°C; sev‐PkPk/allele of interest were grown at 25°C. w1118 was used as control.
Histology and immunohistochemistry
Tangential sections were prepared as described (Tomlinson and Ready, 1987). Stainings of imaginal disks were performed as described (Weber et al., 2000; Feiguin et al., 2001), mounted in Vectashield (Vector Laboratories) and viewed on a Leica confocal microscope. Stacks of five to nine optical sections were assembled with NIH Image and Adobe Photoshop. Rat anti‐ELAV (a gift from G.Rubin) was used at 1:100, anti‐Pk antibody (a gift from J.Axelrod) was used at 1:500 (Tree et al., 2002) and anti‐Fmi (a gift from T.Uemura) was used at 1:10 (Usui et al., 1999). Mouse anti‐βGal (Promega) was used at 1:200, and rabbit anti‐βGal (Cappel) was used at 1:2000.
Molecular biology and biochemistry
A detailed description of the constructs used is given in the Supplementary data. The SMART program was used to predict transmembrane domains and known motifs (Schultz et al., 1998). Multiple sequence alignments were made using Clustal X (Thompson et al., 1997). All PCR products were either sequenced or replaced by original cDNA fragments. Silent changes were ignored.
GST fusion proteins were expressed and purified as described, except that the protein expression was induced at 30°C (Breitwieser et al., 1996). Radiolabeled proteins were translated in vitro with the T7‐coupled transcription–translation system (Promega) according to the manufacturer's instructions.
For GST pull‐down experiments, 5 μg of the appropriate GST fusion protein was incubated with 15 μl pre‐equilibrated glutathione–Sepharose beads (Pharmacia) in 500 μl BP [50 mM Tris–HCl pH 7.6, 150 mM KCl, 0.5% TritonX‐100, 1 mM dithiotheitol and 1× EDTA free protease inhibitor cocktail (Roche)] for 30 min at 4°C on a rotating arm. The beads were then washed three times with 1 ml BP and incubated with 5 μl of an in vitro translation reaction in 500 μl for 1 h at 4°C. The beads were then washed three times with 1 ml BP and resuspended in 20 μl 2× concentrated SDS sample buffer. Samples were then analyzed on a 12% or 15% SDS–polyacrylamide gel. The gel was fixed, dried and exposed to a Biomax film (Kodak).
Xenopus manipulations
Eggs were obtained and fertilized in vitro by standard methods and staged as described (Nieuwkoop and Faber, 1967). Cleavage stage embryos were injected in 0.5× MMR with 3.5% Ficoll using an Oxford micromanipulator and a PLI‐100 Pico‐Injector (Harvard Apparatus). Dorsal and ventral blastomeres were distinguished at early cleavage stages by pigmentation. Capped RNA for injection was produced in vitro from linearized plasmid using the Message Machine kit (Ambion). When phenotypes were analyzed, 1 ng RNA was injected. One hundred picograms of the GFP‐fusion RNAs were used in the localization studies. Animal caps were cut at stage 10–10.25 using standard techniques and placed in 0.5× MMR under a coverslip with a thin layer of vacuum grease around the edge of the cover. The caps were viewed live using a Nikon Optiphot fluorescent microscope.
Supplementary data
Supplementary data are available at The EMBO Journal Online.
Note added in proof
Bastock et al. have also recently reported an association of Stbm and Pk [ OpenUrl].
Supplementary Information
Supplementary movie [emboj7595334-sup-0001.avi]
Legend to movie [emboj7595334-sup-0002.pdf]
We thank Carol Bodian for her help with the statistical analysis, D.Gubb, T.Wolff and the Bloomington stock center for fly strains, J.Axelrod and T.Uemura for antibodies, and Micheal Burnett, John Suriano, Adriene Scola and Zhong‐fang Du for technical help. We thank R.Krauss, E.Purdue, E.Wurmbach and members of the Mlodzik laboratory for critically reading the manuscript. The work has been supported by NIH grant RO1 GM62917 to M.M. A.J. was supported in part by Swiss National Science Foundation grant 823A‐64689, and R.S.D. by NIH MSTP grant GM07739. Confocal microscopy was performed at the MSSM‐Microscopy Shared Resource Facility, supported with funding from NIH‐NCI R24 CA095823–01, NSF MRI DBI‐9724504 and NIH S10 RR0 9145–01 grants.
- Copyright © 2003 European Molecular Biology Organization


text: Turf's up: Hart vs. Elbert Thu, 08/18/2016 - 4:00am It's a new field and new scoreboard, but Friday night, it will be the same old rivalry as Hart County ...PLEASE LOG IN FOR PREMIUM CONTENT. Our website requires visitors to log in to view the best local news. Not yet a subscriber? Subscribe today! Read more about Turf's up: Hart vs. Elbert


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Commentary | Another Dick Vitale Gala brings hope thanks to two kids named Lauren Hill and Dillon Simmons
Lauren Hill and Dillon Simmons were two kids who loved sports and wanted to enjoy life.
Their time was cut short.
Cancer has a way of doing that, but what these two youngsters did gives a sense of immortality to their lives.
They will be honored at the 10th annual Dick Vitale Gala on May 15th at the Ritz-Carlton Sarasota. The gala has raised more than $12 million for pediatric cancer research since its inception nine years ago.
This year's grants will include $250,000 in memory of Hill and $200,000 in memory of Simmons.
They inspired those who knew their stories, especially other youths who were fighting a similar battle. They showed us most of our daily complaints are trivial.
Hill and Simmons didn't get enough time to live out their dreams, but they gave hope to thousands who are continually caught up in the war against cancer.
Hill was just a high school kid who wanted to play college basketball. She signed a scholarship to play for Mount St. Joseph University in Cincinnati, but before she could thoroughly enjoy that moment, she was diagnosed with brain cancer.
She took a path that many of us would find difficult to navigate, fighting her disease while relentlessly working to raise money for pediatric cancer research so others could beat the enemy that was closing in on her.
Hill captured the nation when she scored the first and last baskets in her team's season opening victory. She played in four more games raising awareness of diffuse intrinsic pontine glioma (DIPG), a rare form of pediatric brain cancer.
Hill passed away at the age of 19 last month. She was awarded the Pat Summitt Most Courageous Award given out by the United States Basketball Writers Association and was honored during the past Jimmy V Women's Basketball Classic.
Mount St. Joseph women's basketball coach Dan Benjamin said Lauren made a huge impact on his life and the lives of his players in the short time they knew her.
"She was one kind of remarkable woman, very unselfish and always wanting to give to her teammates and the younger kids who had the disease, but couldn't speak out," Benjamin said.
"She was a great voice for DIPG. She was an unselfish caring and giving. She was witty and had a great sense of humor. And she could play basketball. She said if this was going to happen (her life ending) she wanted to do it the right way and help others."
Hill and Simmons lived those famous words Jimmy Valvano bestowed upon us when he was fighting cancer 22 years ago: "Don't give up. Don't ever give up."
His words gave birth to the Jimmy V Foundation, which raises money for cancer research and will be in our backyard again Friday.
Numerous celebrities from the sports world will be in attendance, but as we learn every year, the kids who are on the front lines of this battle are the superstars.
"Lauren Hill inspired so many and amazed me by relentlessly raising money for research while courageously battling brain cancer," said ESPN college basketball guru Dick Vitale. "That, my friend, is a very special young lady. Lauren will never, ever be forgotten."
Simmons was passionate about hockey and played for the Florida Eels in Lee County. In September 2012 after a collision on the ice, medical tests revealed he had a golf ball-sized tumor in his brain.
There were three surgeries and more than 30 chemo treatments. He recovered enough to play hockey again and attended the 2013 Vitale Gala. He passed away three weeks before the 2014 gala at the age of 15.
He was named the most positive student at this middle school after his first surgery, and his fighting spirit was an inspiration to all who knew about his plight.
"Young Dillon Simmons, who we had the good fortune to have at our eighth annual gala, so impressed us with his determination to fight until he unfortunately lost his life to brain cancer, too," Vitale said. "As we give grants in both Lauren's and Dillon's name, we will make certain that others will benefit from research."
This year's gala will honor Tampa Bay Bucs head coach Lovie Smith, Syracuse head basketball coach Jim Boeheim and Florida State football coach Jimbo Fisher.
People can make donations to the Dick Vitale Gala (c/o The V Foundation 106 Towerview, Cary, N.C., 27513) or by calling 1-800-4JIMMYV.
Alan Dell, Herald sports writer, can be reached at 941-745-7056. Follow him on Twitter @ADellSports.


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The Worcester Blades faced Les Canadiennes de Montreal on Saturday night where Montreal won by a score of 7-1, leading in shots on goal 45-18.
Lauren Dahm got the start for Worcester and Montreal countered with Marie-Soleil Deschenes.
Les Canadiennes were missing seven players from their roster due to the USA/Canada Rivalry Series. Ann-Sophie Bettez, Marie-Philip Poulin, Hilary Knight, Erin Ambrose, Emerance Maschmeyer, Jill Saulnier, and Genevieve Lacasse combine for 160 points for Montreal this season.
First Period
Montreal quickly retrieved the puck, and scored 21 seconds in. This came from Catherine Daoust. Her second of the year had assists from Karell Emard and Katia Clement-Heydra.
Courtney Turner answered with two solid shots on net about a minute later, but couldn't find the back of the net.
After some defensive play turned into an offensive opportunity, Taryn Harris drew interference penalty on a partial breakaway. The Blades power play did not bring a tie score after sustaining the majority of the zone time during the two-minute advantage.
Dahm came up with a nifty glove stop to keep her team in it with 13 minutes remaining in the first. She followed this up with multiple split saves in a row.
Worcester tied the game up briefly with a goal from Kaitlin Spurling for her first of the season. Alexis Miller notched the assist.
Kaitlin Spurling ties the game on tip out front!!! @WorcesterCWHL ties the game at 1! #CWHL pic.twitter.com/7VBoF5Phc7
— Women's Sports Highlights (@WSportHilites) February 17, 2019
The Blades would see this quickly fade away though as Montreal scored two back-to-back goals 23 seconds apart. The first came from Emard with Melanie Desrochers notching the second.
Worcester headed into the locker room down by two goals.
Second Period
Fujimagari entered the net to start the second.
Harris came in close to the net with a shot early, but Deschenes made the save.
The Blades continued with some offensive pressure as K. Spurling got an open shot off, but Deschenes made the block.
Shortly after, Tutino received a pass from Emmanuelle Blais and dangled around Fujimagari to score on a half open net at the 10:40 mark.
Clement-Hydra notched her second point of the game, scoring Montreal's fifth goal two minutes and 46 seconds later.
Both teams saw opportunities, with Fujimagari making a notable right-to-left save, preventing another score.
FUJIMAGARI STONES DAOUST! What a save by Fujimagari to keep the game at 5-1. @WorcesterCWHL #CWHL pic.twitter.com/DYUIC9hiK1
— Women's Sports Highlights (@WSportHilites) February 17, 2019
That's how the score remained, and both teams headed to the final intermission with Montreal leading in shots on goal 28-14.
Third Period
One minute and 35 seconds in, Melodie Daoust scored her eighth of the year assisted by Emard.
Morgan Turner took a tripping penalty, causing the Blades to go on their first penalty kill of the game.
M. Daoust kept it up, scoring her second and Montreal's seventh of the game on the power play at the 7:27 mark. Emard notched another assist along with Lauriane Rogeau.
Demi Crossman took an interference penalty at the 11:54 mark, but the Blades played strong defensively and prevented another score.
With under three minutes remaining, Fujimagari made a series of stops and covered to slow down play in her defensive zone. The Blades followed this up with some brief offensive zone time, but couldn't notch a second goal.
The clock wound down and the Blades fell in this one by a final score of 7-1.
Three Stars
1 – Kayla Tutino (Montreal)
2 – Mélodie Daoust (Montreal)
3 – Kaitlin Spurling (Worcester)
Next Game
The Blades take on Les Canadiennes one final time this season on Sunday, February 17 at 12:30 p.m. Tickets can be purchased online or at the door on game day.
Merisa Boyd is the Director of Communications for the Worcester Blades. All media inquiries and requests should be sent to [email protected]


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Frederick Wilhelm Flato and Sophia
Wilhelm Flato and Sophie Welhausen were married 05 Jan 1848 in Austin County, Texas. By 1850, in Austin County, Texas they had daughter Eliza Flato.
In 1860, in Fayette County, Texas they had 6 children: Eliza, Charles, William, Joseph, Rudolph, Henry, Alma
In 1870, in Fayette County, Texas they had 8 children William , Henry, Rudolph, Alma, Edward, Paul, Oscar, Max Flato.
In 1870 there is an Eliza Jersig (22 Texas) and a J. Wm Jersig (28 Austria) in the Flato home.I believe this is J. Wenzel Jersig and wife Eliza as the ages and dates match the 1880 Wenzel (38) and Eliza (29) Jersig ages and birth sites.
In 1880, Sophia and daughter Ann Flato liviing in the L. P. and Sophia Amsler home in Fayette. And sons Charles and Henry Flato were living there with their wives and children. In the F.P. and Alma Yeager home were found Paul, O. C., and Herman (8 Max?)Flato. Rudolph and Edward Flato were not found in the 1880 Fayette County, Texas. Did they die or move away.
I believe the 1870 Census taker listed Eliza (Flato) Jersig with her Flato siblings and then her husband J. Wm. Jersig at the bottom of this family.
More Replies:
Re: Frederick Wilhelm Flato and Sophia
Mike Kelly 7/18/05


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|B.B.A., M.B.A., B.Sc. VISUAL COMMUNICATION., B.Sc. ANIMATION AND VISUAL EFFECTS & DIPLOMA IN VISUAL EFFECTS (MOU) Programmes
|Faculty of Marine Science
|B.F.Sc Programme
|Faculty of Engineering and Technology
|B.Voc ResultS Nov-2018 (01.03.2019)
|M.Ed & D.Pharm. & Pharm.D. & B.E. (Part Time) & DIPLOMA IN MINING ENGINEERING Programmes
|M.E (Regular & Partime)., M.Tech., M.C.A Programmes (02.02.2019)'
|B.E Programmes Result on 10/01/2019
UG Engineering & Pharmacy (Regular & Part - Time), Examination results are published at University Website (www.coe.annamalaiuniversity.ac.in). Students can apply for any clarification of the results and revaluation of theory paper, through the respective Head of Departments with a prescribed fee Rs. 2000/- per theory paper on or before 25.01.2019.
|Faculty of Agriculture
|M.Sc. (Agri.) / M.Sc.(Horti.) (04.03.2019) / B.Sc. (Agri.) / B.Sc.(Horti.) / B.Sc. (Hons.) Agri / B.Sc. (Hons.) Horti / Dip in Agri / Dip in Horti Programmes (02.02.2019)


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An opportunity to see old friends, make new friends, talk a little shop, or just catch up. The Walrus & AAF Columbus will host November's happy hour for industry professionals.
Back to All Events
Earlier Event: November 28AMA:Job Transition Strategies - Job Search Beyond the Job Board
Later Event: December 2Short North Holiday Hop


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Using Social Media to Build your Business
Having an online presence is key for any business in 2018 but choosing which social media platform to use and knowing what and when to post can be tricky.
We've compiled a few top tips for using social media to your advantage to build a good reputation and gain more members!
Know your target audience and know which social media platform they favour. Generally, it's probably best to be on most platforms to reach the biggest audience – we've listed the key few and how they can be used…
- Facebook – great for paid ads and reaching the older generation, whether this is your target audience or you're looking to get the parents attention, generally Facebook is used by slightly older people.
- Instagram – Instagram has a huge fitness following and is a great place to start your social media campaign. Instagram is based around posting photos, so this is great if you get new facilities, your team competes or you're trying something new!
- Twitter – Twitter is less about photos and more about short, sharp one-liners. With a limit on characters for each post, this area is for people looking for information quickly – it's a great place to reach a lot of people and to post links.
- Pinterest – Again, mostly aimed at the older generation, this is a place for links, guides and workout plans. It has a huge fitness following but I wouldn't spend too much time on this social media platform – it's aimed at a DIY attitude and is a place where people will come to look for at home workouts etc and although this will build your brand, it probably won't bring too much new custom.
- Snapchat – Mostly used by the younger generation and maybe has a bit of a bad name but it's great to reach the audience of younger people in your area. Most businesses now have a Snapchat account, and whilst it can be a bit daunting at first you'll soon get the hang of it and it's all about fun on Snapchat. Remember you can do paid ads here too.
Paid ads –
Most social media platforms offer an option to promote your posts or put out an advert – all for a cost of course. This doesn't have to cost the earth to be effective. Ensure you're choosing your platform wisely and narrowing your target audience to people that are in your area and maybe already interested in your area of business.
When to post –
Do your research on when to post! If you're using Facebook, the best time to post is between midday and 4pm and the worst is either early or late in the day on a weekend. However, posting late evening works best for Instagram. Just because you've taken a great pic or have a great post idea, maybe wait for the most effective time to post.
What to post –
All the studies show that the accounts that post regularly have the most the most page views and click throughs, so maybe aim for once a day or every other day, but keep it regular! Remember that whilst you take your business seriously, social media should be a fun place and is a chance to show off what your business has to offer. Whether this is before and after weight loss photos, your students going to the next belt grading or a member showing off what they've achieved, it's all about positivity.
Hashtags and Location pins –
Ever thought of creating a hashtag unique to your business? This means that anyone searching this hashtag can view not only your posts but your members posts too, which looks great for a potential members. Anything you post should also have your location pin attached, where possible, this means anyone searching your area will come across your post.
Keeping it up to date –
All of your social media pages should have your up to date contact information and a link to your website and to a joining page. If someone comes across your page, likes what they see and wants to be a member, it should be easy for them to join right away. Have you got this facility available? Here at SimplyCollect we offer an unlimited amount of joining pages and prospect trials for your business, for free!
SimplyCollect can help you both gain new members and manage your current members with a comprehensive and easy to use software package that can allow new members to sign up online and current members to track their progress – all whilst managing the membership administration, direct debit payments and arrears. To find out more about how we can help grow your business and manage your current workload, contact us on 0330 223 3152 or at firstname.lastname@example.com or find out more here.
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Conan O'Brien is done with NBC, so reports are saying. NBC has yet to officially confirm this news, but that's the word on the street/Internet. Conan's reign is scheduled to end next Friday, January 22nd. NBC had scheduled a hiatus for the show that was supposed to start the 25th, but in light of all of this crazy bullshit, Conan's taking off a few days early.
What was the final straw? I guess NBC/Universal president & CEO Jeff Zucker was so cold and nasty throughout the negotiations that Conan became too turned off to consider going back. From Nikki Finke's blog:
And while Jeff Zucker has not stepped up and taken responsibility for this mess he set in in motion in the first place — by replacing Leno with Conan O'Brien as host of The Tonight Show even though Jay was No. 1 in his time slot at the time — he's been busy behind the scenes. I've already reported how Zucker has been privately blaming Conan for the current debacle, saying "He let me down" because The Tonight Show for the last 7 months since O'Brien took over has been losing out to David Letterman in both eyeballs and advertiser-coveted demographics. (Bullshit, Zucker, you can't keep blaming others for your Zuck-ups.) Bad enough that Zucker made Conan hear about the planned move in the first place from the media.
One rep even compared Zucker to "Darth Vader" because the NBCU chief "has been so evil" about this. His 3 1/2-years threat comes because O'Brien has another 2 1/2 half years left to run on his contract, and NBC could also enforce a clause that keeps Conan off television for a year or more after that.
The backlash from Conan fans all over the Internet (it's been somewhat of a craze the last few days) probably don't help the fact that NBC's ratings have been at an all-time low recently.
I'll keep you updated as more details (and a confirmation) come in…


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Stunning broad views. Ready to build. House plans approved for a 4000 sq. ft home. 500' of gorgeous rip rapped shoreline. Mature trees and sunrise views.
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3 Tips To Flawless Skin In Time For Summer
Posted by Stem Organics Admin on
Hallelujah, summer is almost here! After a cold winter we are ready for a season full of warmth, sunshine and of course many beach days!
Now is the time to prepare your skin before the weather starts to really heat up. Steamy days means products that will help you to look radiant, but never greasy. You want products to balance the oil levels in your skin, but still keep it perfectly hydrated.
Read on to find out 3 of our best tips for a flawless skin this summer!
Get Your Drink On
Before you start celebrating, we aren't referring to that sneaky margarita, but rather to pure, filtered water.
Water is important all year long, but it becomes essential when out in the heat of summer. Apart from the obvious health implications when your body becomes dehydrated, your skin suffers too. Many people may suffer from mild dehydration and not even know it! Dehydration shows up as dull, dry and flakey skin, and you can often blame sugary drinks such as soda pop for causing this. You may think that sugary pop or tea quenches your thirst, but in fact, sugar draws water out of your cells leading to dehydration which shows up as less than flawless skin.
To achieve that flawless skin you want, be sure to sip on water throughout the day, and always have a glass alongside alcohol or a sweet drink.
Treat Your Skin To Some Extra TLC With A Weekly Face Masque
All those sweaty days quickly leads to a buildup of dirt and grime in your pores. Clogged pores can lead to annoying breakouts, and that's the last thing you want during summer when you are trying to go lighter on the concealer.
Our Perfect Complexion Face Masque provides your skin with a powerful dose of vitamin C and kaolin clay to fight inflammation and draw dirt out of your pores, leaving your skin clearer, brighter and beautiful!
Don't Skip The Moisturiser
It may be tempting to skip the moisturiser when your skin might feel greasy all day, but this will simply cause your skin to overproduce oil, and lead to breakouts.
Choose a moisturiser that is right for your skin, and stay away from heavy formulas when hitting the beach. Try our Balancing Face Fluid to control sebum if you have an oily complexion, or our Exquisite Face Fluid for all skin types.
Follow our foolproof tips and enjoy gorgeous, flawless skin all summer long without any stress!
Share this post
- Tags: dry skin, moisturiser, organic skincare, skin, stem, stem organics, Summer skin


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Oz, a TV personality made famous by Oprah Winfrey. The fruit is known in India as gambooge. It is apparently also an ingredient in some weight loss products as hydroxycitric acid.
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Instant Rescue Support by the Global air Ambulance in Kolkata with the ICU Availability
Here we are available with the re-innovated support of the Emergency and the Non-Emergency patient shifting facility by availing the facility provided by Global Air Ambulance Services in Kolkata. We are availing the best experience to the patients with the process of the medical tourism with acquiring the best emergency handling faculty of the specialist doctors and the supportive medical staff to avail the required observation and to support with the medical treatments to stabilize their situatcontinue reading →
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Rochelle Humes has it all… famous hubby, gorgeous baby, successful career, and now… a backstage pass to the JLS boys' final tour and the most epic night out with her fellow WAGs.
The singer and mum-of-one, who's rumoured to be in talks for a role on Loose Women year, posted a series of intimate snaps to her Instagram page last night, showing off her access all areas pass and her girls' night out backstage with the boys.
With each of the JLS WAGs dressed in colours coordinated to their partners (remember back when JLS started, and they only wore red, green, blue and yellow? Oh yes), the girls took snaps to show off their fancy tees, each of which bears the name of their man. So jealous.
But cutest of all is definitely baby Alaia-Mai's tee. The tiny tot sported her 'Green Princess' (aww, you guys) t-shirt with a huge black tutu, black tights and shoes. SO on-trend.
Rochelle tweeted the picture with the caption: 'Girls just want to have fun,' before sharing another selfie snap backstage with her fellow JLS girls.
JB Gill later took to Twitter to thank the girls for their support, saying: 'Love that the ladies came to see us @ChloeTangney @sarahsugar84 @AJAzari @RochelleTheSats and baby Alaia! #JBontour x'
Well, we officially want to date a boybander if it means we get one of those t-shirts. #justsaying.
By Rebecca Martin
Got a tablet? You can now download LOOK magazine straight on your iPhone, iPad, Kindle (just search the store for 'LOOK magazine'), Nook or Zinio. And don't forget to rate and review!


text: 20 May Posted at 02:59h in Uncategorized by kimhyland 0 Comments 0 Likes Share "In Your book were all written the days that were ordained for me, when as yet there was not one of them." Psalm 139:16All my days. They were all written. Even before one of them came to be. You wrote my story. You know the... Read More


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Hershoran, P B — Accountants in North York, Ontario, Canada
Full information about Hershoran, P B in North York, Ontario, Canada: phone. address, website, fax, opening hours, etc.
Contact Information
Address: 1200 Sheppard Ave E, North York, Ontario M2K 2S5, North York, Ontario (ONTARIO), M2K 2S5
Phone: (416) 495-7070
Category: Accountants in North York
Hershoran, P B opening times
Sun: Closed
Mon: 10:00am - 4:00pm
Tue: 10:00am - 4:00pm
Wed: 10:00am - 4:00pm
Thu: 10:00am - 4:00pm
Fri: 10:00am - 4:00pm
Sat: Closed
* Hours "Hershoran, P B" indicated for the examination and may not coincide with the real!
You can specify the time by phone (416) 495-7070
Reviews about Hershoran, P B in North York
About Hershoran, P B
"Hershoran, P B" plain in our web-site in category Accountants in North York. For a letter, use the address 1200 Sheppard Ave E, North York, Ontario M2K 2S5, North York, ONTARIO M2K 2S5. On this page you can find detailed information about the "Hershoran, P B". You can call the company by phone Hershoran, P B (416) 495-7070
The nearest Accountants in Ontario
1110 Sheppard Ave E, North York, Ontario M2K 2W2, North York, Ontario, M2K 2W2(416) 222-8803
1200 Sheppard Ave E, North York, Ontario M2K 2S5, North York, Ontario, M2K 2S5(416) 494-8466
1210 Sheppard Ave E #304, North York, Ontario M2K 1E3, North York, Ontario, M2K 1E3(416) 499-3211
220 Duncan Mill Rd #416, North York, Ontario M3B 3J5, North York, Ontario, M3B 3J5(416) 445-9110


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Well the good news is no mange. The bad news is she does have a spectacular case of ear mites and tape worms. Luckily they can both be treated but she won't be getting many snuggles and NO kisses for the next three weeks until the medicine has time to work its magic.
The vet thought she was probably a year to a year and a half old, had surprisingly good teeth for her condition and needed to put on some weight. She weighed in a at a paltry six pounds. She weighs less than either of our cats.
On second thought, maybe I do need to give her some kisses. Perhaps I could benefit from a tape worm. Nothing else is working.
I bought her a cute little pink collar with a bell on it. I had to get a cat collar because her neck is so dang skinny. It's cute listening to her prance around in it.
So far, so good.


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EMMANUELLI FILMS (identity + poster design)
Siblings Mariana & Joserro Emmanuelli are award-winning short film writers and directors. Uara created the identity for their company Emmanuelli Films and has also designed for several of their projects.
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"What Nigerians Should Consider When Voting" – Ben Murray-Bruce
Senator Ben Murray-Bruce representing Bayelsa East has cited what Nigerians should put into consideration when voting for the next president in 2019.
Ben Bruce said the religion and tribe of a presidential candidate is not essential in selecting next president but the capability of the candidate.
The Bayelsa lawmaker on his Twitter page also urged Nigerians to compare their condition of living since 2015 when APC administration took over power. Bruce wrote:
"Are you better off today than you were in 2015? A simple question. Are you able to eat better today than in 2015? Are you able to look after your health better today than in 2015? These are questions that should guide who we vote for in 2019, not who's from our tribe or religion
"If you are ill, you want the best doctor to treat you. You do not care if he is Black, Brown or White. You just care that he is capable. Nigeria is sick. We don't need a Northern or Southern President or a Christian or a Muslim President. We just need a capable President in 2019."
The post "What Nigerians Should Consider When Voting" – Ben Murray-Bruce appeared first on LATEST NIGERIAN NEWS BREAKING HEADLINES NEWSPAPERS.


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A new journey or a new phase of our life is about to come. Yes, we are going to enter in a New year i.e. 2020 January Calendar. The first month of this coming new year is about to come. So for those who want to plan their vacations in this coming year. One thing they must need is Printable Calendars.
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So , now some of you might be confused why you need printable calendars. So, Let me clear you this thing. As obvious, A new year Is coming up and you guys must have some plans to go out somewhere and explore new places. Some of you might be thinking to take a break from your work and to go to meet you families in your hometown. So, for that which thing you must need to do is to make a schedule. Where and When you can go according to your working schedule?
2020 January Calendar Printable
So, To make a schedule for your vacations, you must need to have a printable calendar along with you. This thing will help you a lot, as we have some plans always. But, we can't make out as we don't schedule it and when we get to know it's a holiday. Then you do not want to take off from your work because you do not want deduction in your salary. Ultimately, the plan gets fail.
2020 January Calendar With Holidays
One thing which I want to tell you people, Never avoid your holidays. Make some plans with your friends and family.
Just Go Out and Have fun!! Because the working life is going on as always. But what moments that we spend with our family and friends will become the most memorable part of our lives.
January 2020 Calendar PDF Template
You need a calendar for various reasons.
- To keep checking your upcoming holidays, you must need a printed calendar along.
- To make a vacation plan, you must need a calendar.
- To check a long weekend, you should need a calendar.
- To check the availability of dates as well as days, you need a calendar.
To carry a printable calendar with you to help you in many ways. So, carry a calendar in a printable format always with you. Which always keeps your remind your vacation plan is about to come and makes you wait for that moment.
January 2020 Blank Calendar Template
Our new year starts with the very first month i.e. January. So, before downloading any month calendar you should have a January printable calendar. So, you can start making plans for you and your family. Because if you want to have a pleasurable life, you need to take out some time for you and for your loved ones.
January 2020 Excel Calendar Template
Only working for an hours will make your life worthless and after some while you will not be happy with your life . So just Checkout the holidays coming in the January 2020, and do make some plans. Here is a January Printable calendar. You can download it for your convenience.
Keep planning & Be happy!!