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Net-A-Porter has gone into sale (up to 50% off, pls note). You guys always seem to love these posts, based on those handy stats; so I thought I'd do a simple list of my fav sale pieces you can snap up. Get that kettle on - enjoy:
- A simple, summery top - but still black, for all my fellow inner goths out there - by Ganni
- The Acne Velocite at 30% off?! I appreciate it's the worst time of year to purchase, but think back to being freezing AF last winter - yeah, that. This is not a piece I've seen on sale before - and you'll wear season after season.
- A few Off-White pieces: awesome pouch, beautiful dress (love those straps), killer cropped shirt, versatile & flattering skirt.
- Anything & everything from R13
- Metallic cowboy boots? Um, yes.
- An off-the-shoulder silk jumpsuit from Zimmerman that is all kinds of perfect...wedding attire, sorted.
- One of the best designer bags I own: Saint Laurent at 30% off. YUP.
- If you need anything a little fancy, Self-Portrait have some killer, ultra feminine pieces (that can also easily be toughened up, if ya like)
- Vetements logo boots: not for the faint of heart. Adore.
- Classic Versace - love this versatile silk scarf - wear around your neck, wrist, around a bag handle, as a crop-top, head-scarf - to name a few.
- Chill T-By Alexander Wang shirt dress. Awesome w/ a leather jacket & some combat boots.
- I may not be massive on prints, but Rixo London do them bloody well: see this silk dress.
- Band tees: my fav go-to of all time? Quite possibly. Made to have that vintage feel (as this truly is the best when it comes to band tees): 30% off.


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Device lowers BP in patients with difficult-to-treat hypertensionApril 6th, 2011 - 2:51 pm ICT by ANI
Washington, Apr 6 (ANI): A new research has revealed that a device developed to treat people with resistant hypertension helped lower blood pressure by 33 points, a substantial drop that would otherwise require patients to take an additional three or four drugs.
The device, called the Rheos System, is the first to be tested in a large-scale clinical trial for the treatment of hypertension and works by activating the body's natural blood flow regulation mechanism to reduce high blood pressure.
The pivotal trial included 265 patients with resistant hypertension treated at 40 medical centers in the United States and two in Europe, and is the latest in a series of studies that have shown the device is beneficial.
People who do not respond to the typical treatment regimen for high blood pressure, which includes one to three medications, coupled with improved nutrition and exercise, have resistant hypertension. These patients are at a far greater risk for stroke, heart attack, heart failure, kidney disease and death, which is why new therapies like Rheos are needed.
"Current drugs and lifestyle modifications can only do so much. I treat a huge number of people who are doing everything right - taking their medications, maintaining a healthy diet, working out - and they still develop resistant hypertension," said John Bisognano, a lead study investigator from the University of Rochester Medical Center.
The Rheos System, developed by CVRx Inc. of Minneapolis, regulates blood pressure much like a pacemaker regulates heart rhythm. A battery-powered implantable generator is inserted under the skin near the collarbone and two leads, or wires, run from the generator to the carotid arteries, the two main arteries that supply blood to the head. The device triggers specific receptors on these arteries, known as carotid baroreceptors - key regulators of blood flow in the body.
These receptors then send signals that are interpreted by the brain as a rise in blood pressure. The body responds to this phantom rise in blood pressure by taking action to lower it, including relaxing the blood vessels and reducing the heart rate.
"The device lowers blood pressure in a way that actually benefits patients beyond changing their numbers - it improves the structure of the heart which in turn improves overall cardiac function," said Bisognano.
The study was presented at the American College of Cardiology's Annual Scientific Sessions. (ANI)
- Why hypertensive people's BP increases during exercise - Apr 05, 2011
- Resistance exercise beneficial for cardiovascular health - Jan 16, 2011
- Resistance training good to lower blood pressure - Jan 17, 2011
- St. Jude launches device for treatment of hypertension - May 15, 2012
- Powerful molecule regulator in blood pressure control system discovered - Mar 12, 2010
- Brisk walk 'can boost brain blood flow in elderly women' - Apr 13, 2011
- Pine bark extract improves kidney function in patients with metabolic syndrome - Mar 02, 2011
- More strokes, fewer heart attacks with carotid stents: Study - Oct 12, 2010
- Solar-powered device reliable tool to measure blood pressure: Study - Nov 09, 2010
- Ultrasound technique better alternative to invasive angiography for diagnosing heart disease - Jun 15, 2010
- New surgical procedure lowers blood pressure - Jan 18, 2012
- Heart patients watch out, winter can be deadly - Dec 19, 2010
- Hormone may help reverse serious pulmonary condition - Sep 16, 2011
- New technique to identify potential atherosclerosis risk found - Apr 12, 2011
- New proteins that regulate blood pressure, flow identified - Jul 08, 2010
Tags: blood flow regulation, carotid arteries, cvrx inc, heart failure, heart rhythm, high blood pressure, kidney disease, lifestyle modifications, lower blood pressure, main arteries, pivotal trial, regulation mechanism, resistant hypertension, rochester medical center, substantial drop, supply blood, treatment of hypertension, treatment regimen, typical treatment, university of rochester medical center

subset: commoncrawl


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A prime use case in process mining is hence to abstract this complex data set to a visual representation, a process model, highlighting frequent behavior, bottlenecks, or any other data dimension that might be of interest to the end user.
Data Science of Process Mining – Understanding Complex Processes. Previous post. Next post Tags: What is Process Mining? Process Mining is a relatively young technology, which was developed about 15 years ago at the Technical University of Eindhoven by the research group of Prof. Wil van der Aalst. Given the name, it seems to be related to the much older area of 'data mining
Iron processing, use of a smelting process to turn the ore into a form from which products can be fashioned. Included in this article also is a discussion of the mining of iron and of its preparation for smelting. iron; production of steelIron ore is one of the most abundant elements on Earth, and
Simplify Complex Process Maps#182;. Have you imported a data set in your process mining tool and what you got was a complex "spaghetti" process? Often, real life processes are so complex that the resulting process maps are too complicated to interpret and use.
This process is a challenge and very difficult by applying the business rules in the joined tables and decision makers to get the informative knowledge from complex data. The
Process Mining techniques in complex Administrative Processes 3 2.2 Minit Minit is a process mining tool by which we can import datasets for a wide range of
Gold cyanidation (also known as the cyanide process or the MacArthur Forrest process) is a hydrometallurgical technique for extracting gold from low grade ore by converting the gold to a water soluble coordination complex.
How Does Bitcoin Mining Work? By Euny Hong Updated February 27, 2018 — 5:25 AM EST . Share. Cryptocurrency mining is painstaking, expensive, and only sporadically rewarding. Nonetheless, mining has a magnetic draw for many investors interested in cryptocurrency. This may be because entrepreneurial types see mining as pennies
When you hear about bitcoin mining, you envisage coins being dug out of the ground. How do they do this? By solving a complex mathematical puzzle that is part of the bitcoin program, and including the answer in the block. The puzzle that needs solving is to find a number that, when combined with the data in the block and passed through a
As it turns out, mining for diamonds is one of the most resource heavy and time consuming process that companies have to invest their efforts in. Even with all our technological advances and engineering tools, diamond mining still incorporates a certain level of art and science combined. So, let us also take a look some methods in which
Process Mining techniques in complex Administrative Processes 3 2.2 Minit Minit is a process mining tool by which we can import datasets for a wide range of
Gianluigi Greco University of Calabria Process Mining in Complex Domains joint work with Antonella Guzzo and Luigi Pontieri Information (Computational) Systems in
Bitcoin mining—the process of generating new units of the which is used to solve the complex mathematical Newsweek/ MapChart "As mining can provide
Data mining techniques. Martin Complex data mining benefits from the past experience then input it to the rest of the data mining process,
Process Mining is introduced and explained, including Its benefits for Data Science, and key resources for further exploring Process Mining, including videos,
Cross industry standard process for data mining, the deployment phase can be as simple as generating a report or as complex as implementing a repeatable data
Data mining is the process of discovering actionable information from large sets of data. Data mining uses mathematical analysis to derive patterns and trends that exist in data. Typically, these patterns cannot be discovered by traditional data exploration because the relationships are too complex
Mieke Jans, Michael G. Alles, and Miklos A. Vasarhelyi (2014) A Field Study on the Use of Process Mining of Event Logs as an Analytical Procedure in Auditing.
How Process Mining Compares to Complex Event Processing Anne 28 Jul. Complex Event Processing (CEP) is a topic that has gained more and more interest over the past years. The core idea is that huge event streams are correlated and analyzed on the fly, for example, to detect fraud patterns or monitor stock prices.
In this lecture we explain what lasagne and spaghetti processes are, and what you can(not) do on the different types of processes.
Business Process Mining Promises Big Data Payoff. behind the scenes business processes get more complex. Process mining starts with data discovery.
The revolutionary process mining technology in Disco can create that can deal also with complex and heterogeneous process by Fluxicon Process
Read chapter 3 Technologies in Exploration, Mining, and Read chapter 3 Technologies in Exploration, Mining, The separation process is often complex
Data Mining Tools: Compare leading Help convert existing data sets into the proper formats necessary in order to begin the mining process. Cluster complex and
Data Mining Tools: Compare leading Help convert existing data sets into the proper formats necessary in order to begin the mining process. Cluster complex and
Within healthcare there are thousands of complex and variable processes that Using process mining The key process mining technique of process discovery
Lana Labs – Process Mining. LANA enables the simple analysis and visualization of complex events in your business processes by using process mining techniques.
Process mining techniques allow for extracting information from event logs. For example, the audit trails of a workflow management system or the transaction logs of an enterprise resource planning system can be used to discover models describing processes, organizations, and products.
The rise of cryptocurrency mining . Cryptocurrency mining describes the process by which persons and/or businesses with high powered computers and servers compete against one another to solve highly complex mathematical equations that are the result of the encryption designed to protect transactions on a blockchain network.
The Geology of Rare Earth Elements Geology.com. Republished from: The Principal Rare Earth Elements Deposits of the United States The formation of alkaline rocks is complex and not fully understood but can be .
Clinical Intelligence, Complex Event Processing and Process Mining in Process Aware EMR / EHR BPM Systems
The Process of Solving Complex Problems formal definition and a process theory of CPS conducted with a focus on empirical data mining rather than
Pattern based Mining in Electronic Health Records for Complex Clinical Process in Electronic Health Records for Complex process mining, complex
How does Bitcoin work? This is a question that often causes confusion. Here's a quick explanation! The basics for a new user. through a process called mining.
Planning an Iron Ore Mine: From Exploration Data to Informed Mining example of a complex adaptive that can be treated individually during the mining process).
Managing Complex Transformations Achieving excellence February 2009 A summary of our transformation management and programme leadership capability in the mining
more complex multidimensional analysis, Data analysis and data mining tools use quantitative Data mining can be defined as the process of extracting
Simplify Complex Process Maps; Take Different Perspectives On Your Process; Run a Process Mining Project; Disco User Guide. Process Mining in Practice
The rise of cryptocurrency mining . Cryptocurrency mining describes the process by which persons and/or businesses with high powered computers and servers compete against one another to solve highly complex mathematical equations that are the result of the encryption designed to protect transactions on a blockchain network.
Lana Labs – Process Mining. LANA enables the simple analysis and visualization of complex events in your business processes by using process mining techniques.
(Aurelien Foucault for Quartz) Bitcoin mining is 24/7 Scientists have figured out exactly how much you need to exercise to slow your heart's aging process.
For Genesis Mining, the process of bitcoin mining could be described as a meeting of fire and ice. Mining — the method by which new bitcoins are made by a computer solving an extremely complex math puzzle — requires big, energy intensive computing power to
Abstract. Visual Mining refers to the human analytical process that uses visual representations of raw data and makes suitable inferences. During this analytical process, users are engaged in complex cognitive activities such as decision making, problem solving, analytical reasoning and learning.
The commissioning of the mining and concentration complex at Russia's Vladimir Grib field, for which Tenova Bateman CIS supplied the process solutions and equipment for the treatment plant, marks the successful delivery of the project from concept to production.
A Complex Mining Process about Air Quality Mihaela Juganaru Mathieu1, Silvia Gonz#225;lez Brambila2 1Ecole Nationale Sup#233;rieure des Mines, Saint Etienne, France 2Universidad Aut#243;noma Metropolitana,Azcapotzalco, M#233;xico D.F, M#233;xico
Process mining software helps Vodafone get an inside look at its SAP business processes to improve Vodafone is a complex business that now sells
Managing Complex Transformations Achieving excellence February 2009 A summary of our transformation management and programme leadership capability in the mining
Feb 09, 2018China's huge bitcoin mining China's unwanted bitcoin miners may move it's mined by computer algorithms that solve increasingly complex
The Refining Process. Photo and content courtesy of CVR Energy. Generally, The most complex refineries add coking, more hydrotreating and hydrocracking.
Coal mining is only a temporary use of land, modern mining personnel must be highly skilled and well trained in the use of complex,
Few investors have a firm grasp of the length of time environmental studies and the approvals process requires. Having visited numerous investor forums, we real
Become the best Bitcoin miner and learn how to mine Bitcoins with the best Bitcoin mining hardware, Bitcoin mining is the process of adding transaction records to


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The Spring Breakfast held on Saturday, April 22 was a tremendous success with approximately 125 stopping by for a hearty breakfast at Mission Clubhouse. Thank you to all who supported this event. Special thanks to our amazing volunteers, Jim, Gail, Andrea, Beverley & Reg, for their time and efforts, as well as Gerry, Lori, Michael, Scott, Janet, & Hank.
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Eighteen years ago I held my oldest daughter for the very first time. I had no idea things would turn out as they did, but I still remember the bundle of blankets and her tiny face with the serious eyes that I imagined were looking into mine. It was scary to have her drop so much weight, had I known then what I do now, things would be different, but all the books I read hadn't prepared me for the tiny person that went home with us. I say us because he was there, but one of the loneliest feelings was being in labor while he and my mother were in the room with me. Thankfully things were very different the second time around, but that's a story for another day.
I had no idea how tired I truly was. Today I took a nap, the long, powerful kind where you wake up with marks on your face and a dim idea of what planet you are on, what season it is, and what day of the week it might be. I have a three day Pilates streak going and I'm marveling at the fact that I was too tired to do a video that took less than fifteen minutes, and in some cases even less time, but it's true. Perhaps I am, or was, depressed, but now I'm convinced that being perpetually and chronically sleep deprived might have driven me and others into clinics and psychiatric facilities when lack of sleep was one of the main underlying issues.
I'm so happy today. My heart feels light, I'm more carefree than I can ever remember being, and I'm sure a large part of it is finally being able to start catching up on my sleep. I tackled some of the paperwork that was in my inbox, paid off a bill for one daughter, made a dent in another one, and marveled at how I am still being the responsible one, trying to pick up the pieces of our lives long after ink on my divorce dried up and started fading. I deleted the dating app for now. I may go back to it at some point in time, but I'm tired of the whole thing. It feels like work, like a game, where two people are more suspicious of each other than attracted to someone, and that's just not my jam.
I've had some really vivid dreams, both featuring the father of my children. In one I am on some sort of large vessel, might be a boat, could be a plane, and I'm told I need to get going only to find that there are an insurmountable number of possessions that I need to pack, and nobody else is there to help me. I do my best as people come along to be snide or upbraid me, I have another child I have never seen before, in my dream this sort of makes sense, then I see another girl, and question her, then realize that she isn't my daughter, but apparently the third girl is and I am now responsible for her too. Initially she is a baby, then she's quite a bit older. Strange indeed.
The dream I had earlier tonight involved an older house full of all sorts of junk. This turns into a version of the store where I used to work. I'm cleaning and organizing and wondering why he has two places when I only have one. He stops by with his girlfriend, I made a little Elmo thing for her and she doesn't want to accept it, but I insist, we hug, and then we go back to these two gay men, one of whom tells me that he has the knife that he does because of me. I use it to slice some chicken and remain impressed with the quality and craftsmanship. In the previous dream I found some fabulous clothes for my aunt, this doesn't have that type of closure, I'm happy when I awake and realize none of it was real.
Much love,
P.S. I can't remember the last time my mood was this good, as if nothing and nobody could bring me down. I'm so happy I left my job when I did. I think being fresh and relaxed going into the next opportunity is going to make all the difference in the world.
Until next time,
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- Research
- Open Access
In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2
© Berencsi et al; licensee BioMed Central Ltd. 2011
- Received: 15 September 2010
- Accepted: 30 March 2011
- Published: 30 March 2011
Infiltration of colorectal carcinomas (CRC) with T-cells has been associated with good prognosis. There are some indications that chemokines could be involved in T-cell infiltration of tumors. Selective modulation of chemokine activity at the tumor site could attract immune cells resulting in tumor growth inhibition. In mouse tumor model systems, gene therapy with chemokines or administration of antibody (Ab)-chemokine fusion proteins have provided potent immune mediated tumor rejection which was mediated by infiltrating T cells at the tumor site. To develop such immunotherapeutic strategies for cancer patients, one must identify chemokines and their receptors involved in T-cell migration toward tumor cells.
To identify chemokine and chemokine receptors involved in T-cell migration toward CRC cells, we have used our previously published three-dimensional organotypic CRC culture system. Organotypic culture was initiated with a layer of fetal fibroblast cells mixed with collagen matrix in a 24 well tissue culture plate. A layer of CRC cells was placed on top of the fibroblast-collagen layer which was followed by a separating layer of fibroblasts in collagen matrix. Anti-CRC specific cytotoxic T lymphocytes (CTLs) mixed with fibroblasts in collagen matrix were placed on top of the separating layer. Excess chemokine ligand (CCL) or Abs to chemokine or chemokine receptor (CCR) were used in migration inhibition assays to identify the chemokine and the receptor involved in CTL migration.
Inclusion of excess CCL2 in T-cell layer or Ab to CCL2 in separating layer of collagen fibroblasts blocked the migration of CTLs toward tumor cells and in turn significantly inhibited tumor cell apoptosis. Also, Ab to CCR2 in the separating layer of collagen and fibroblasts blocked the migration of CTLs toward tumor cells and subsequently inhibited tumor cell apoptosis. Expression of CCR2 in four additional CRC patients' lymphocytes isolated from infiltrating tumor tissues suggests their role in migration in other CRC patients.
Our data suggest that CCL2 secreted by tumor cells and CCR2 receptors on CTLs are involved in migration of CTLs towards tumor. Gene therapy of tumor cells with CCL2 or CCL2/anti-tumor Ab fusion proteins may attract CTLs that potentially could inhibit tumor growth.
- Chemokine Receptor
- Tumor Cell Apoptosis
- Autologous Tumor Cell
- Apoptotic Tumor Cell
- Fetal Fibroblast Cell
Chemokines play an important role in immune homeostasis and immune surveillance (reviewed in [1–3]). Studies have demonstrated that chemokines influence immune reactions by regulating trafficking of dendritic cells (DC) and lymphocytes [4]. In tumor bearing individuals, the role of chemokines is paradoxical. Chemokines produced by tumor cells are known to stimulate autocrine tumor growth, progression and metastasis [4–10]. In contrast, chemokines produced by tumor cells can also attract chemokine receptor (CCR)-positive leukocytes into the tumor area, potentially leading to tumor growth inhibition in vitro and in vivo [9, 11–13]. In colorectal carcinoma (CRC) patients, T-cell infiltration has been shown to be associated with good prognosis (reviewed in [14] and [15–19]). In these studies, favorable prognosis was correlated with the presence of tumor cells secreting chemokines such as CCL5, CXCL10 and CXCL1 that played a role in recruitment of CCR5+/CXCR3+ T-helper (Th)1 cells or CX3CR1+, perforin+/granzyme B+ T cells [17], [18]. Colorectal and pancreatic carcinoma cells are known to secrete CCL2 which is associated with increased tumor infiltration of macrophages [20–22]. However, there are mixed reports of good and bad prognosis due to increased infiltration of tumor associated macrophages in these studies [20–22]. In those studies, the level of tumor derived-CCL2 and its influence on T cell infiltration of tumor cells is unclear. In mouse tumor model system, there are indications that melanoma cells secreting high amounts of CCL2 attract macrophages resulting in tumor growth inhibition [23]. In another study, transfection of mouse CT-26 CRC cells with CCL2 gene resulted in decreased metastasis and increased susceptibility of tumor cells to macrophage lysis [24]. Thus, selective modulation of chemokine activity at the tumor site could attract immune cells resulting in tumor growth inhibition (reviewed in [25] and [1]). In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor rejection, which was mediated by infiltrating T cells at the vaccine site. Infiltration of T cells into the tumor area was followed by rejection of both transduced and non-transduced tumor cells [11–13]. CD4+ T-cell subsets have been implicated in tumor rejection induced by vaccination of mice with CCL19-transduced tumor cells [26], and CD8+ CTL were instrumental in tumor growth rejection in mice following intratumoral delivery of CCL20 or CXCL12 via adenovirus vectors [27] or injection of CCL16-expressing tumor cells. Both CD4+ and CD8+ T cells were required for tumor growth inhibition to occur in mice injected intratumorally with CCL21 [28].
It has been suggested that immunological intervention of cancer patients has been largely unsuccessful due to limited ability of T cells to infiltrate tumors in vivo ([29, 30]). Chemokines fused to anti-tumor Ab may be utilized to attract adoptively transferred tumor antigen (Ag) -specific T cells to the tumor site [31]. To develop immunotherapeutic strategies for cancer patients based on chemokines and their receptors, similar to the approaches already successfully used in mice, one must identify chemokines and their receptors involved in T-cell migration toward tumor cells.
Recently, we have shown in an organotypic culture system (reconstruct) that migration of CTL derived from a CRC patient towards autologous tumor cells was mediated by chemokine receptor CXCR3 expressed by the T cells, and CXCL11 chemokine secreted by the autologous tumor cells [32]. In the present study, we show that migration of CTL derived from another CRC patient is dependent on CCL2 and CCR2.
Materials and Methods
Cell lines
CCR2 expression by T-cell lines established from tumor infiltrating lymphocytes of CRC tissues.
T cell
Expression of CCR2
(% positive cells)b
Dukes' Disease Stage
The following monoclonal antibodies (mAb) were used: mAb Nok-1 to Fas ligand (BD-PharMingen, San Diego, CA); mAb CH-11 to CD95 and anti-CD11a mAb (Immunotech, Westbrook, ME); fluoresceinated (FITC) or phycoerythrin (PE) -conjugated anti-CD:4, 8, 25, 29, 40, 40L, 44, 49a, 49b, 54 and 80; anti-CXCL-11 mAb; anti-human CCR:1, 2, 3, 5, 6, 7 and 9 mAb; anti- CXCR:1, 2, 3, 4, 5 and 6 mAb (R&D Systems, Minneapolis, MN); anti-human CCR:4, 8, and 10 mAb (Imgenex, San Diego, CA); anti-CCR11 and -CX3CR1 polyclonal Ab (Abcam, Cambridge, MA); FITC conjugated goat anti-mouse IgG (Invitrogen, Carlsbad, CA). Recombinant human CXCL11 was purchased from R&D Systems.
Chemokine determination by RT-PCR or ELISA
mRNA was extracted from CRC cells (5 × 106) using Fast Track 2.0 mRNA isolation kit (Invitrogen). The primers used were 5'-GCC CGG TGT CAT CTT CCT AAC CAA GC-3' and 5'-AGG GGA CAG GGG AAC TCT CAG AGC AA-3' for CCL3; 5'-TGC TGC TTT TCT TAC ACC GCG AGG AA-3' and 5'-AGA AGG GAC AGG AAC TGC GGA GAG GA-3' for CCL4; 5'-TCT GCA GCA CTT CTG TGT CTG-3' and 5'-GGA TCC TAG AAG GAG CTG GA-3' for CCL7; 5'-CAG TCC ATG AGA AGG AGT CCA-3' and 5'-AGA TCC TGC ACA GGA CTG TG-3' for CCL8; 5'-AGG GCA TGG GTT TTA TTA TAT ATA TAT-3' and 5'-TTT AAA AAT AAC TGA TAT TCA TGG-3' for CCL11; 5'-TCA TCT TTC CAC AAT AAC ATA TTT A-3' and 5'-GTT TAT TTG AGT ATT GCT GAT CTT T-3' for CCL13; 5'-GGA CTT CCT GGA TCC TCC TC-3' and 5'-AGC AGT CAG CAG CAA AGT GA-3' for CCL15; 5'-ATG GCC CTG CTA CTG GCC CTC AGC CTG-3' and 5'- TTA ACT GCT GCG GCG CTT CAT CTT GGC-3' for CCL19; 5'-TGT AGG GCG ACG GTT TTA-3' and 5'-TCC ACC ACA ACA TGC AG-3' for CCL25; 5'-GGC CCT GCC CTT ATA GC-3' and 5'-CTA ACT TGG GGT TGA CAT T-3' for CXCL1-3; 5'-TGT TGA GAG AGC TGC G-3' and GGG TTC AGA GAC CTC CA-3' for CXCL5; 5'-GAA GTG GTA GCC TCC C-3' and 5'-GCT TTC CCC CAC ACT C-3' for CXCL6; 5'-TCC GCT GCA TGT GTA TAA AG-3' and 5'-ATA GGT ATC CTG AAT AAA TGA GAA C-3' for CXCL7; 5'-CAT GCT GGT GAG CCA AGC AGT TTG AA-3' and 5'-CAC TTC TGT GGG GTG TTG GGG ACA AG-3' for CXCL9; 5'- CGA TGC CTA AAT CCC AAA TCG AAG CA-3' and 5'-AAT TGC TGG ACT CCT TTG GGC AGT GG-3' for CXCL11; 5'-ATG AAC GCC AAG GTC GTG GTC-3' and 5'-TGG CTG TTG TGC TTA CTT GTT T-3' for CXCL12; 5'-TCT CTC CAG GCC ACG GTA TTC-3' and 5'-ACC ATT TGG CAC GAG GAT TCA C-3' for CXCL13. CCL21 primer was purchased from Biosource (Camarillo, CA) and CCL2, CCL5, CXCL8 and CXCL10 primers were purchased from R&D Systems. PCR reactions were performed for 35 cycles (94°C, 45 sec; 60°C for CCL4, CCL19, CCL21, CXCL1, CXCL5, CXCL6, CXCL7, CXCL9, and CXCL11; 56°C for CXCL12, 55°C for CCL2, CCL3, CCL5, CCL25, CXCL8 and CXCL10; 52°C FOR CCL7 and CCL15; 48°C for CCL8, CCL11 and CCL13, 45 sec; 72°C, 45 sec) using the SuperScript One-Step RT-PCR kit (Invitrogen). All PCR involved an initial denaturation step at 94°C for 45 sec to 1.5 min and a final extension step for 7 min at 72°C. All PCR products were analyzed using 10% novex-TBE gel (Invitrogen). Supernatants obtained from CRC cells on day 6 of culture were tested for the presence of CCL2, CCL3, CCL15, CCL19, CCL21 and CXCL11 using ELISA kits (R&D Systems).
Phenotyping of tumor cells and T cells was performed as described [32]. In brief, cultured cells were incubated with saturating concentrations of FITC or PE-conjugated mAb (5 μg/ml) detecting human lymphocyte and tumor markers in FACS buffer for 1 h at 4°C, followed by excess mAb removal by washing in FACS buffer. Binding of the mAb was analyzed as described [32].
T-cell migration in organotypic CRC culture (reconstruct)
Organotypic CRC cultures were initiated as described [32]. In brief, 1.8 × 105 fetal fibroblast cells were mixed with collagen matrix (450 μl) and plated in a 24-well tissue culture treated plate (Corning, Corning, NY). After 24 h, WC007 CRC cells (1 × 105) were seeded on top of the collagen layer and after 24 h, a separating layer of fibroblasts in collagen matrix (100 μl, 500 μm) was added on top of the CRC cells. CTLs (1-10 × 105) were mixed with fibroblasts (1 × 105) in 250 μl collagen matrix and plated on top of the separating layer. In some cultures, CRC cells were stained with CellTracker Blue CMAC (15 μM, for 40 min at 37°C; Invitrogen) and CTL were pre-stained with CFDA-Green (5 μM, Invitrogen). For control reconstruct, autologous PHA blasts (PBMC stimulated with PHA [1% v/v, Invitrogen] and propagated in recombinant interleukin (IL)-2 [20 U/ml; a gift from the Biological Resources Branch, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD] for 3-4 weeks) were used. Reconstructs were incubated in medium (50% DMEM, 50% CRC medium supplemented with 2% human AB serum). On various days after addition of T cells reconstructs were fixed and processed for histological evaluation as described earlier [32]. The percentage of apoptotic tumor cells was determined by counting apoptotic nuclei and intact tumor cells in sections stained with H&E.
Blocking of T-cell migration in reconstruct
T-cell migration in the reconstruct was performed in the presence of anti-chemokine or chemokine receptor Abs (10 μg/ml) or isotype-matched control Ab added above the separating layer, followed by addition of CTL-fibroblast collagen layer [32]. To evaluate whether excess chemokine can block migration of T cells, the chemokine (50 ng/ml) was added into the medium on top of the T-cell layer. The percentage of apoptotic tumor cells in the presence and absence of inhibitor was determined and the percentage of inhibition of apoptosis by Abs or chemokines was calculated [32].
Chemotaxis assay
CTL migration was evaluated using a 24-well, Transwell plate (8.0-μm pore size; Corning, Corning, NY) as described earlier [34]. In brief, T cells were washed once with RPMI1640 medium, cell count re-adjusted (5 × 105 cells/mL) in T cell medium [33] and an aliquot (100 μL) of T-cell suspension was placed in the top chamber of the Transwell. Bottom chamber of Transwell plate received chemokine (500 μL in T cell medium) at the indicated concentration prior to the addition of T cells in the top chamber. After 90 min incubation at 37o C in a 5% CO2 atmosphere, the top chamber was removed, and the number of T cells that had migrated into the bottom chamber was counted under the microscope.
Formalin-fixed paraffin-embedded sections (5 μm) were deparaffinized by sequential application of Sub-xylene substitute (3 × 10 min, Surgipath Medical Industries, Richmond, IL) and re-hydrated through a graded series of ethanol after which they were rinsed shortly in phosphate buffered saline (PBS). In situ end labeling (ISEL) was performed as described earlier [35]. Briefly: sections were air-dried and digested with proteinase K (1.25 μg/ml in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0) for 30 min at 40°C in a humidified chamber. After incubation, sections were rinsed in distilled water followed by sequential changes of ethanol (70-95%), and air dried. Sections were end-labeled by incubating with biotinylated dCTP, dATP, and non biotinylated dTTP, dGTP (0.01 mM each, Invitrogen) in the presence of DNA polymerase Klenow I fragment (Promega Corporation, Madison, WI) for 60 min at 40° C. Sections were blocked later for endogenous peroxidase activity with H2O2 (0.3% in methanol, for 20 min at RT, serially rinsed with distilled water and PBS) and the biotin-labeled DNA sequences were detected by horseradish peroxidase (HRP)-conjugated streptavidin for 40 min at 37° C. Slides were washed and incubated with 2', 5'-diaminobenzidine (DAB, Vector Laboratories, Burlingame, CA) followed by counterstaining with hematoxylin. Caspase staining was performed using an Ab specific for the active form of caspase 3 (present only in cells undergoing apoptosis). In brief, sections were blocked for endogeneous peroxidase as above, followed by addition of avidin/biotin and protein blocking using respective blocking kits (Vector Laboratories and Immunotech). After blocking, slides were incubated with rabbit anti-human active caspase 3 polyclonal Ab (1:1500 dilution, R & D Systems), at 4°C overnight, followed by incubation with biotinylated anti-rabbit Ab and HRP-conjugated streptavidin (both from Vector Laboratories). Signals were visualized with 2', 5' DAB as the substrate. The slides were counterstained with hematoxylin. Normal rabbit gamma globulin was used as a negative control (MP Biomedical Services, Santa Ana, CA).
Statistical analyses
Differences between experimental and control values were analyzed for significance by 2-sample Student's t-test.
Functional characteristics of CTL007 in reconstruct
Apoptosis induction by CTL007 in reconstruct with autologous WC007 CRC cells*
Total number of tumor cells mean ± SD/field (10 fields)
Number of apoptotic tumor cells mean ± SD/field (10 fields)
Percentage of apoptotic cells, mean ± SD/field (10 fields)
Experiment I
80.5 ± 38.8
23.5 ± 8.7
31.7 ± 11c, d
PHA blast
136.2 ± 54.8
15.9 ± 6.8
12.1 ± 3.9c
No lymphocytes
90.1 ± 3.5
7.1 ± 2.8
8.1 ± 3.5d
Experiment II
116.7 ± 19.4
94.8 ± 19
79.5 ± 6.7a, b
PHA blast
118.3 ± 23.6
34.7 ± 11.8
27.5 ± 4.7a
No lymphocytes
121.1 ± 22.3
25.8 ± 10.7
21.3 ± 10.1b
Migration of CTL was visualized in reconstructs using T cells labeled with CFDA-Green and tumor cells labeled with CMAC-Blue. Lymphocytes migrated from the top layer of collagen and fibroblasts through a separating layer of collagen and fibroblasts toward WC007 tumor cells (Figure 1B [h]). Although a small proportion of PHA blasts also migrated to the tumor cell layer, the PHA blasts did not induce significant apoptosis in the tumor cells (Figure 1B [a, c, e, g; Table 2).
Phenotypic characteristics of CTL007 and WC007 CRC cells
Phenotypic and functional markers of anti-CRC CTL007 and autologous WC007 tumor cell line
Parameter investigateda
Cell lines (% cells positive)
HLA Class I
HLA Class II
< 1
< 1
< 1
< 1
CD49a (α1 integrin)
CD49b (α2 integrin)
CD29 (β1 integrin)
< 1
CD11a (LFA-1a)
< 1
WC007 CRC cells express both HLA class I and II molecules, FAS, ICAM-1, and various integrins. Expression of α2 and β1 integrins by the CRC cells may facilitate their binding to collagen [42]. CRC cells also express FAS ligand (< 19%) and are positive for B7-1 and ICAM-1. B7-1 and ICAM-1 on the CRC cells potentially interact with CD28 and LFA-1a on the CTL, respectively, which may result in T-cell stimulation [43].
Thus, several phenotypic markers are expressed by CTL007 and WC007, which are known to facilitate interactions between these cells and between the lymphocytes or tumor cells and collagen or fibroblasts in the reconstruct, leading to activation of T cells as well as T-cell migration toward tumor cells.
Chemokine and chemokine receptor involved in CTL007 migration toward WC007 cells
Chemokine receptors expressed by CTL007, and chemokines produced by WC007 CRC cells
Chemokine receptors expressed by CTL007a
Chemokine receptors
% positive
Known to bind
to receptor
Expressed by WC007 b
ELISA (pg/ml)
< 30c
< 30c
< 20c
< 30c
Induction of tumor apoptosis by CTL007 is inhibited by anti-CCR2 and CCL2 Abs and excess CCL2a
Blocking agent
Number of
tumor cells/field
(10 fields)
Number of apoptotic cells/field (10 fields)
% of apoptotic cells
% of tumor cell apoptosis inhibition
21.8 ± 9.7
8.2 ± 9
40.5 ± 9.7b
Control IgG
21 ± 2.2
8.4 ± 1.1
40.1 ± 4.9c
Anti-CCR1 Ab
22 ± 3.4
10.0 ± 1.6
45.6 ± 5.0
Anti-CCR2 Ab
19.6 ± 1.1
2.6 ± 0.5
13.2 ± 2.3c
Anti-CCR3 Ab
22.4 ± 2.8
9.2 ± 1.9
40.7 ± 4.2
Anti-CCR5 Ab
21 ± 2.6
9.6 ± 1.8
45.5 ± 3.8
Anti-CCR7 Ab
18.8 ± 2.4
7.2 ± 1.6
38.0 ± 4.1
Anti-CXCR3 Ab
21.4 ± 1.5
9.2 ± 0.8
43.1 ± 1.8
21.4 ± 4.4
3.6 ± 1.1
17.2 ± 6.1b
Anti-CCL2 Ab
17.8 ± 2.6
2.2 ± 0.4
12.7 ± 3.8c
CCR2 and CCL2 expression by T cells and tumor cell lines, respectively, derived from additional CRC patients
We investigated the distribution of CCR2 and CCL2 in T cells and tumor cell lines, respectively, established from specimens of additional CRC patients. Expression of CCR2 receptor and its ligand by the cells of additional CRC patients would suggest that involvement of the receptor/ligand in T-cell migration toward tumor cells may not be a unique observation made in a single CRC patient, but may be found in other patients. Ten tumor reactive T-cell lines derived from TIL of additional CRC patients were analyzed for CCR2 expression and four of these showed CCR2 expression (Table 1). Furthermore, two of the established CRC cell lines produced CCL2 (data not shown).
We have demonstrated here that CTL007 migrate through a 500 μm collagen/fibroblast separating layer toward tumor cells, resulting in tumor cell apoptosis. We have also shown that migration is dependent on CCR2 expressed by T cells and CCL2 secreted by tumor cells.
Our recently developed novel three-dimensional culture system offers a unique way of studying migration of leukocytes toward tumor cells and the factors that influence leukocyte migration under physiological conditions [32, 34]. As described in our previous studies, human CRC is grown in vitro under three-dimensional conditions using a mixture of collagen and fibroblasts [32, 44]. Interaction of α2 and β1 integrins on CRC-specific T cells with collagen and the presence of activated fibroblasts help to maintain Ag-specific T cells in a state of activation in absence of exogenous addition of IL-2 [36, 37]. In addition, T cells could interact with fibroblasts via adhesion molecules like LFA-1a, ICAM-1 and CD44 which could in turn stimulate fibroblasts to secrete inflammatory cytokines such as IL-1, IL-6, IL-7 [38, 39], and fibronectin [41]. IL-1 could stimulate T cells to express IL-2 receptor and induce secretion of IL-2 [45]. IL-6 and IL-7 are T-cell survival factors [46], and fibronectin stimulates predominantly resting lymphocytes [41]. Other investigators have used collagen matrices to study interaction of leukocytes with tumor cells, but they have not demonstrated CTL migration resulting in tumor cell apoptosis in a culture system similar to the reconstruct cultures shown here [47–49].
In the present study, CCL2 produced by CRC cells attracts CTL through binding of CCL2 to its corresponding chemokine receptor CCR2 on the T cells. This was demonstrated by blocking of T-cell migration in presence of addition of excess chemokine CCL2 in the T-cell layer of the reconstruct or the addition of Abs to CCL2 or CCR2, each applied on top of the separating collagen/fibroblast layer. Involvement of CCR2 in T-cell migration was further confirmed in chemotaxis assay in Transwell plate experiment by treating T cells with anti-CCR2 Ab. Although CTL007 has several chemokine receptors matching chemokine produced by CRC cells, predominant expression of CCR2 (65%) by the T cells and the relatively higher amount of CCL2 secretion by tumor cells may have been a decisive factor in T-cell migration.
Many carcinomas, including breast, colorectal, pancreatic and renal carcinomas, and neuro-ectodermal tumors such as melanomas, medulloblastomas, neuroblastomas and glioblastomas are known to produce CCL2 (reviewed in [1]; [20–22, 50]. CCL2 secretion by tumor cells can aid in tumor progression, angiogenesis and metastasis [1, 5, 10]. Also, the secretion of CCL2 by tumor cells results in infiltration of tumor cells by leukocytes including T cells, NKT cells and macrophages (reviewed in [1]; [20–22]). To our knowledge, the role of CCL2-dependent T-cell migration in CRC is largely unknown. In mouse tumor model systems, melanoma cells secreting high amounts of CCL2 attract macrophages resulting in inhibition of tumor growth. However, tumor cells secreting low amounts of CCL2 promote tumor growth by stimulating angiogenesis [23]. In another study, tumor cells transfected with CCL2 showed decreased metastasis due to increased infiltration of macrophages and susceptibility of tumor cells to lysis by infiltrating macrophages [24]. Thus, patients may be vaccinated with chemokine-transduced tumor cells [51] or tumor-associated Ags fused to chemokines [52]; alternatively, patients may be treated with anti-tumor Ab/chemokine fusion protein, which may attract adoptively transferred lymphocytes to the tumor area [12, 52, 53]. In light of the mouse study by Nesbit et.al., [23], one needs to carefully modulate the expression of CCL2 to attract immune cells toward tumor cells. Thus, chemokines may be useful for immunotherapy of cancer patients.
In addition to therapeutic implications, the results of our study have prognostic potential. Infiltration of CRC with T lymphocytes is correlated with a favorable prognosis [54], and chemokine receptor expression by T lymphocytes as well as chemokine production by tumor cells should be explored for their possible association with prognosis. Identification of tumor cells secreting CCL2 or T-cells expressing CCR2 in the tumor microenvironment could be a useful prognostic marker in CRC patients [17, 18, 22, 55–58]. In our earlier study, we have shown that migration of CTL derived from a CRC patient towards autologous tumor cells was mediated by CXCR3 expressed by the T cells, and CXCL11 chemokine secreted by the autologous tumor cells [32]. In the present study, we show that migration of CTL is dependent on CCL2 and CCR2. Presence of CCR2 in T cells obtained from four of the ten additional patients suggests that CCR2 expression may not be that uncommon. It is likely that in each individual CRC patient a unique chemokine/chemokine receptor pair might be involved in attraction of T cells towards tumor cells. Hence, it is essential to identify the chemokine/chemokine receptor pair responsible for attraction of T cells towards tumor in each CRC patient for individualized therapy.
Our study demonstrates the role of CCR2 and CCL2 in migration of CTLs towards tumor. Data obtained from additional CRC patients further strengthen the role of CCR2 and CCL2 in lymphocyte migration. Identification of chemokine/chemokine receptor pair responsible for attraction of T cells towards tumor in each patient will aid individualized therapy approaches using gene modification of tumor cells with chemokine or chemokine/anti-tumor Ab fusion proteins to attract CTLs that potentially could inhibit tumor growth.
We thank James Hayden and Frederick Keeney for technical help on microscopy imaging and Jeffrey S. Faust, David Ambrose and Daniel Hussey for assistance in flow cytometry analyses. We thank Elsa Aglow and Russell Delgiacco for providing assistance in histotechnology. We also thank Drs. Yingtao Bi and Ramana Davuluri for statistical data analyses. This work is supported by National Institute of Health grants CA74294, and CA10815, by a grant from Corixa Corporation, by Intramural National Cancer Institute Funds, and by the Commonwealth Universal Research Enhancement program, Pennsylvania Department of Health.
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Bone broth is a nutrient rich broth you can make at home with your table scraps! It provides easily absorbed calcium, phosphorus, magnesium and potassium. Bone broth also contains amino acids, glucosamine and collagen. Bone broth can help you heal from infections more easily, It may help heal leaky gut, decrease joint pain, prevent bone loss and help you have healthy skin, hair and nails.
Bone broth
Use organic bones and produce as much as possible.
- 4 quarts water
- 1 teaspoon salt
- 2 tablespoons apple cider vinegar
- 2 large onions, unpeeled and coarsely chopped
- 2 carrots, scrubbed and coarsely chopped
- 3 celery stalks, coarsely chopped
- 1 bunch fresh parsley
- Any vegetable skins or peelings you have on hand
- 2-3 garlic cloves, lightly smashed
- 2-4 lbs. meat or poultry bones, these may be roasted before if you wish a richer taste
You will notice I did not have parsley or celery and was still able to make this broth!
Place all ingredients in a large slow cooker set on high. Bring to a boil, then reduce the setting to low for 24-48 hours. The longer it cooks, the better it tastes.
After 12 hours cooking.
After 24 hours cooking.
Strain the stock through a fine mesh strainer or coffee filter into a large bowl, and discard the waste. What bones are left will be very brittle. My chicken carcass had usable meat, so I took that out of the discarded stuff.
Finished strained bone broth.
Use the broth in homemade soups or other recipes. Even if you don't have a slow-cooker you can still reproduce this recipe on a stove top, with a large pot on low heat or in the oven. You can keep the broth in the fridge 3-4 days or in the freezer for up to a year.


text: On 11/18/06, Bill Tomlinson wrote... > How can I remotely monitor the status of my PERC RAID array if I'm > running ubuntu (or debian)? > I've seen some mention of a program from LSI called megarc, but I > can't find an actual copy of it anywhere for downloading. I have the same problem. I'd like to monitor RAID without running OMSA, but have still yet to find a solution. Dell's percsnmpd used to work great, but sadly hasn't been updated for megaraid_sas :-( The PERC5/i is very similar to LSI Logic's 8480E card, so their MegaCli software works just fine. The commands you'll probably be interested in running are... MegaCli -LDInfo -Lall -aALL MegaCli -AdpAllInfo -aALL ...which will return output containing eg... State: Optimal ...and... Virtual Drives : 1 Degraded : 0 Offline : 0 Physical Devices : 5 Disks : 4 Critical Disks : 0 Failed Disks : 0 Among lots of other stuff. I hope this helps. If not try getting Dell's 10892.mib (it's on one of the cd's they ship with the server, or on an FTP site somewhere I'm sure) as that may possibly enable you to monitor RAID via SNMP. I'm still working on that, though :-)


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68.6k questions
260k answers
317k comments
1.3k users
this is ridiculous................ PRDP should be at least a super hit..... BOI said its one of the rarest movies to have recovery of over 200cr. 194cr with 2.3cr footfalls .... and movie itself was distributed by rajshri meaning no loss there as well. these stats speak of blockbuster. and they are giving it HIT. thank you www.srkoi.com


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Sanctity Of Human Life
Contributed by Terry Laughlin on Jan 1, 2018 (message contributor)
Summary: When it comes to human life, God's Word is the final authroity. Scripture is clear, God created mankind and has His hand on them even before birth.
Sanctity of Human Life
God Has the Final Word
The whole of Scripture teaches that mankind is created by God and given the choice to live for Him (Joshua 24:15), to live for self (Galatians 5:19), the world (Mark 4:19) or believe the lies of the devil (John 8:44). The world view and the devil effectively used the selfish flesh to cheapen the value of human life. The whole of Scripture teaches that God created mankind, what He says is the final word.
1.) Genesis 1:26-27 says, "Then God said, "Let us make man in our image, in our likeness, and let them rule over the fish of the sea and the birds of the air, over the livestock, over all the earth, and over all the creatures that move along the ground. So God created man in His own image, in the image of God He created him; male and female He created them." "Created" (?????? bara) an active verb meaning only God can create mankind (Genesis 1:27), heaven and earth (Genesis 1:1), the heavenly host (Isaiah 40:26), the new heaven and earth (Isaiah 65:17) which is yet to come. - Baker, W., & Carpenter, E. E. (2003). The complete word study dictionary: Old Testament (p. 161). Chattanooga, TN: AMG Publishers.) Isaiah tells us God is the creator of this heaven and earth, He gives breath and spirit to the people who live on it. (Isaiah 42:5) The highlight of God's creation is human life, coming forth from Devine deliberation, God the Farther, The Son and the Holy Spirit, "Let us make man." There is a humorist joke about a scientist who told God that mankind no longer needed Him. They are now able to create mankind on their own. God wanted to give the scientist a chance to prove himself, so God invited the scientist to go back to his laboratory and create a human being. As the scientist was leaving, he reached down and picked up a handful of dirt, in which the Lord said, "Hey! You get your own dirt." Human life alone is created in the image of the Trinity. "Image" (????? ?elem) meaning in the likeness of God of which we have the opportunity and responsibility to be an example for the Lord morally and ethically. (Swanson, J. (1997). Dictionary of Biblical Languages with Semantic Domains : Hebrew (Old Testament) (electronic ed.). Oak Harbor: Logos Research Systems, Inc.) There is no confusion in the Holy Scriptures, mankind is made up of both male and female, they alone are to be united in marriage. (Genesis 1:27; 2:20-27; Matthew 19:4-6)
2.) God's guiding hand on human life is in play before birth. God's revelation to Jeremiah, "The Lord came to me saying, 'Before I formed you in the womb I knew you and before you were born I consecrated you...'" (Jeremiah 1:5) God ordains and anoints a person for every work He wants to accomplish. (Huey, F. B. (1993). Jeremiah, Lamentations (Vol. 16, p. 49). Nashville: Broadman & Holman Publishers.; Isaiah 49:1) Romans 8:29, "For those God foreknew He also predestined to be conformed to the image of His Son..." "Foreknew" (p??????s?? progin?sko) used in God's eternal counsel includes all He has considered and purposed to accomplish prior to human history. "Consecrated" (?????? qa?aš) means to set apart, to be treated as holy. (Baker, W., & Carpenter, E. E. (2003). The complete word study dictionary: Old Testament (p. 980). Chattanooga, TN: AMG Publishers.)
God has ownership over all mankind, He created them, He has ordained them and promises to always be with them. It is up to each person to surrender to God's perfect will for them. It is not for us to cheapen a person's life saying they have no value, just because we can not see nor respect them. Life in the womb or out is unique, has purpose, having God's ordained call upon their life. God will have the last word in regard to every human life. Americans need to embrace God's value on human life!
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Prof. Cristina Videira Lopes has been named an IEEE Fellow for her contributions to ubiquitous and immersive programming. This honor is bestowed to nominees whose accomplishments have contributed importantly to the advancement or application of engineering, science, and technology, bringing the realization of significant value to society. Congratulations to Prof. Lopes!
Read more about it here: https://www.ics.uci.edu/community/news/view_news?id=1463January 2019
Prof. David Redmiles is hosting Prof. Tayana U. Conte from the Federal University of Rio de Janeiro, Brazil. Conte will be spending a year long sabbatical at UCI starting in February 2019. Here research interests are in software engineering and HCI.January 2019
ISR Director Prof. Sam Malek gave a Keynote Address titled "The Threat in your Pocket: Trends, Challenges, and Solutions in Mobile Application Security" at the 25th Australasian Software Engineering Conference (ASWEC) and Australasian Software Week (ASW) in Adelaide, Australia in November.November 2018
Prof. Bonnie Nardi's paper "Interaction and Outeraction: Instant Messaging in Action," which was presented at the 2000 ACM Conference on Computer- Supported Cooperative Work (CSCW '00), has been awarded the Lasting Impact Award at the 21st ACM Conference on Computer-Supported Cooperative Work and Social Computing (CSCW '18). The paper was co-authored by Prof. Steve Whittaker, UC Santa Cruz, and alumnus Dr. Erin Bradner (Ph.D. 2001, advisor: Gloria Mark), Autodesk.
Read more about it here: https://www.ics.uci.edu/community/news/view_news?id=1406November 2018
The paper "Observed Appropriation of IoT Technology: A Semiotic Account," co-authored by Ph.D. candidate Bruno Azevedo Chagas, Pontifical Catholic University of Rio de Janeiro (PUC-Rio), Prof. David Redmiles, and Prof. Clarisse S. de Souza (PUC-Rio), received the Best Paper Award at the 17th Brazilian Symposium on Human Factors in Computing Systems in October.October 2018
TECHNOLOchicas video spotlight on ICS alumna Rosalva Gallardo, Ph.D. '12. Watch the video here: https://youtu.be/NLtJoOFb9DkOctober 2018
ISR Director Prof. Sam Malek, Prof. Joshua Garcia, and Prof. Nenad Medvidović (USC) are part of a multi-insitution team that has been awarded a $1.66 million research grant from the National Science Foundation (NSF) for "Constructing a Community-Wide Software Architecture Infrastructure."
Read more about it here: https://www.ics.uci.edu/community/news/view_news?id=1411September 2018
Prof. Cristina Videira Lopes and Prof. Jens Palsberg (UCLA) have been awarded a $1.1 million National Science Foundation (NSF) grant for their work on "NJR: A Normalized Java Resource" in which they will work with collaborators from five countries to build a community resource of executable Java programs.
Read more about it here: https://www.ics.uci.edu/community/news/view_news?id=1431September 2018
Prof. Hadar Ziv and Prof. Sameer Patil (Indiana University Bloomington) have been awarded a $283,961 National Science Foundation (NSF) grant for their research on "Incorporating Sociotechnical Cybersecurity Learning Within Undergraduate Capstone Courses."September 2018
Dr. Joshua Garcia, now an Assistant Professor in the ICS Department of Informatics, presented his paper "Lightweight, Obfuscation-Resilient Detection and Family Identification of Android Malware" at the 40th International Conference on Software Engineering (ICS 2018), held May 27 - June 3 in Gothenburg, Sweden. The paper is co-authored by Ph.D. student Mahmoud Hammad and Prof. Sam Malek. This paper was accepted as an ICSE journal first paper, and was published in ACM Transactions on Software Engineering and Methodology (TOSEM), 26 (3), January 2018.July 2018


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Uh oh.. I think I have been spoilt. I hardly ever sleep in air con, usually i'll just turn on the air con to cool the room but i'll off it before we sleep. But somehow these days the boy's room air con feel less cold! So i've been sleeping in air con for the past two nights.
And i ended up perspiring, turning and tossing, finding it hard to sleep last night in my room w/o aircon 😅😅
Ended up watching live stream of the 非常完美 guy whom i wrote about. He did a live stream with the girl till late night! I always wonder what happens after walking away from the TV show, like are they considered in a relationship then? Or how do they start knowing someone with the pre-requisite of dating in mind lol
Best news in a long while!!


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CD duplication services
Wizbit provide CD and DVD duplication services for 12cm and 8cm circular discs and also for business card CDs and DVDs and have quick turnaround times and ship worldwide.
Website: http://www.wizbit.net
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Date Submitted: 01-Aug-2007
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BuilderallThe Best Digital Marketing Platform
Builderall Business
Builderall AffiliateSkip the Drive My Creations Finance Design Thinking Alternative To Builderall For New People What exactly is inside? Yes. Thousands of pages are currently running on BuilderAll. The creator of BuilderAll is running over 200 lead capture, video & sales pages, plus complete membership sites and an iOS app, all on BuilderAll. Courses and programs. Selling information is a lucrative business model for many. But first, you need to position yourself as an expert in your field and your course/program as the solution to your audience's struggles. What Is ePacket Delivery? $100,000+ According to this poll on their forum, while not everybody does (or chooses to) make money from their site, there are plenty of people making a decent amount of cash selling there. In fact, some people are making over $10,000 a year selling there… To succeed on these platforms it's important to establish a niche. The worst thing you can do as a freelancer is brand yourself as a generalist. Since the competition is so high, you'll want to brand yourself as the only solution to a specific type of client. 3/ Click/Heat Map If you're selling products, either hire a freelancer to do the job right or invest in the equipment that you'll need to take and edit high-quality photos. If you're not sure you can afford professional images, check with local colleges to see if there are students looking to learn and build their portfolios that cost less than well-established professionals. 5. Drop Shipping Google User Research Twitter Keaton Row Misc - Fashion Stylist Jump up ^ "Luxembourg Digital Songs : Page 1 | Billboard". Billboard. Retrieved 2016-04-15. 8.1k Views · View Upvoters · View Sharers · Answer requested by Prabhat Singh and Kanhaiya Phuke How profitable is selling on Etsy: According to Slate the average income of profitable Etsy sellers is $44,900 which comes out to be a bit over $3700 a month. With a growing interest in content marketing, more brands are looking for great writers to create content. The secret to succeeding as a writer is to be an expert in a niche. A lot of writers, try to be generalists writing for a wide range of categories from food to tech. However, having a niche focus as writer allows you to write better content. When you have experience in the niche, you can offer a different perspective to a piece of content. That means you're not just saying the same thing as every other article online. And that's what brands really want to pay for. Your thoughts, experiences and inside information into their niche. GreenGeeks DIY handyman tips. Related services sold by the hour or seasonal blocks (everything from raking leaves, to shoveling snow, lawn maintenance, stacking firewood, carpentry fixes, etc.) Earn cash for searching your favorite sites and then completing surveys. You will be asked to share your opinions on a variety of brands, and get paid for doing so. Sign up with Qmee to start using their free Qmee browser app. 4. Make Money With ClixSense Resume Tips ePom.com August 24th, 2016 at 11:00 am And if you want to find out how to get more email subscribers, watch my YouTube video How To Get More Email Subscribers (And Avoid the Biggest Mistake). Over 20 Different Funnels 9.23.2016 thats All Right i will anthem Editorial Calendar Virgin Media Fibre Broadband & TV + Free £100 Nando's Voucher Read You May Like With my online courses, I have to come up with an outline of what the video is going to be about, plan the shots, hope that I can do it in one take, edit the video, convert the video, and then upload the video. Hey that sounds awesome however seems to be pretty much new as a platform, especially if we have to build some important part of our business on YOUR platform. But before everything, and since we will put our NAME on it, we still need to invest some of our time to learn and test the platform ++ cash for the whitelabel license... Hi Mattew, Shopping Tricks 49 Jobs sorted by: Copyright © 2018 Lightspeed. All rights reserved. Want the really good stuff? First Data Builderall Business | Home Based Online Business Builderall Business | Online Business Llc Builderall Business | Online Business Consultant
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