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Tables for
Volume F
Crystallography of biological macromolecules
Edited by E. Arnold, D. M. Himmel and M. G. Rossmann
© International Union of Crystallography 2012
International Tables for Crystallography (2012). Vol. F, ch. 4.1, pp. 99-100
Section 4.1.1. Introduction
aInstitut de Biologie Moléculaire et Cellulaire (IBMC), Centre National pour la Recherche Scientifique (CNRS), 15 rue René Descartes, Strasbourg, F-67084, France,bUPR 9002, IBMC–CNRS, 15 rue René Descartes Cedex, Strasbourg, 67084, France,cDepartment of Molecular Biology and Biochemistry, University of California, 560 Steinhaus, Irvine, CA 92697–3900, USA, and dMachineries Traductionnelles, ARN, UPR 9002, IBMC du CNRS, 15 rue René Descartes, Strasbourg, 67084, France
Macromolecular crystals are much softer than salt crystals. They contain 50% of solvent on average, with values ranging from as little as 25 up to 90%. A direct consequence is that, although morphologically indistinguishable, crystals of macromolecules differ in many respects from crystals of low-molecular-mass compounds. While the latter exhibit firm lattice forces, are highly ordered, generally physically hard and brittle, easy to manipulate, can usually be exposed to air, have strong optical properties and diffract X-rays intensely, crystals of macromolecules are, by comparison, smaller in size, they crush easily, disintegrate if allowed to dehydrate, exhibit weak optical properties and diffract X-rays poorly. They are temperature sensitive and undergo extensive damage after prolonged exposure to radiation.
Proteins or nucleic acids build up a crystalline scaffold, which may be imagined as an ordered gel with extensive interstitial spaces through which small molecules can diffuse freely. In proportion to molecular mass, large macromolecules establish far fewer packing interactions than do small molecules inside crystalline lattices. Since these contacts are responsible for the integrity of the crystals, this largely explains the differences in properties between the two types of crystals. Thus, liquid channels and solvent cavities are directly responsible for the generally poor diffraction properties of macromolecular crystals. Owing to the large spaces between adjacent molecules and the related weak lattice forces, every molecule in the crystal may not occupy exactly equivalent orientations and positions. Furthermore, because of their structural complexity and their conformational dynamics, macromolecules in a given crystal form may exhibit slight variations in their folding patterns or dispositions of side chains.
However, high solvent content is not as negative as it might appear at first glance. It allows maintenance of the macromolecular structures virtually unchanged from those in bulk solvent. As a consequence, ligand binding, enzymatic and spectroscopic characteristics, and other biochemical features are essentially the same as for the native molecule in solution. In addition, the dimensions of solvent channels are such that conventional chemical compounds, such as ions and heavy atoms, substrates or other ligands, may be freely diffused into and out of the crystals. Thus, many crystalline enzymes, though immobilized, are completely accessible for experimentation through alteration of the surrounding mother liquor.
The intrinsic instability of most macromolecules requires that conditions suitable for crystal growth are those that do not perturb their molecular properties. This explains why crystals must be grown from solutions compatible with the target macromolecules, i.e. within a narrow range of pH, temperature or ionic strength. Finally, because hydration is essential for the maintenance of structure, crystals of macromolecules must be always bathed in the mother liquor, even during data collection (except in the practice of cryocrystallography).
The crystallization of biological macromolecules has often been considered unpredictable, although it obeys the same principles as that of small molecules (Giegé et al., 1995; McPherson et al., 1995; Rosenberger, 1996; Chernov, 2003; McPherson & Giegé, 2007). It is, similarly, a multiparametric process. The difference compared with conventional crystal growth arises from the biochemical and biophysical properties of proteins or nucleic acids, and crystallization methods must take into account these features. The methods described below apply for most proteins, large RNAs, multimacromolecular complexes and viruses (for small oligonucleotides or peptides, crystallization by dialysis is not appropriate). For hydrophobic membrane proteins, special techniques are required (see Chapter 4.2 ).
Crystallization proceeds from macromolecules in solution that `aggregate' upon entering a supersaturated state and eventually undergo a phase transition. This leads to nuclei formation and ultimately to crystals that grow by different mechanisms. Supersaturation is the driving force of crystallization and is defined as the ratio [C]/[S], where [C] and [S] are the initial concentration of the macromolecule and its final concentration at saturation, i.e. its solubility. Nucleation is homogeneous when nuclei form in the bulk of the solution, but heterogeneous when they preferentially form on walls of crystallization vessels, on solid particles (dust, aggregates, seeds), or on the surface of existing crystals. Unlike most conventional crystals, protein crystals are, in general, not initiated from seeds, but are nucleated ab initio at high levels of supersaturation that can reach 200 to 1000% (in what follows and for simplicity, the generic name `protein' is used for macromolecule). It is this high degree of supersaturation that, in large part, distinguishes protein crystal formation from that of conventional crystals. That is, once a stable nucleus has formed, it subsequently grows under very unfavourable conditions of excessive supersaturation. Distant from the metastable zone, where ordered growth could occur, crystals rapidly accumulate nutrient molecules, as well as impurities. They also concomitantly accumulate statistical disorder and a high frequency of defects that exceeds those observed for most conventional crystals.
The different stages of crystallization (i.e. pre-nucleation, nucleation, growth, cessation of growth) can be visualized in a phase diagram (Fig. 188.8.131.52). In short, phase diagrams are divided into undersaturated regions (where proteins are soluble) and supersaturated regions (where protein crystals nucleate and grow) delimited by the solubility curve. The supersaturated region is thermodynamically out of equilibrium and can be divided into three kinetically dependent domains: a precipitation domain (at extreme supersaturation) where macromolecules rapidly separate from solution in a solid state either amorphous or microcrystalline, a domain (at lower supersaturation) where nucleation occurs spontaneously and a metastable domain (at low supersaturation) where nucleation does not occur spontaneously but where crystals grow. This domain is favourable for seeding. The wisdom of the crystal grower will be to take advantage of an overall understanding of phase diagrams for designing crystallization strategies and selecting favourable solvent conditions (Sauter, Lorber et al., 1999; Asherie, 2004).
ReferencesAsherie, N. (2004). Protein crystallization and phase diagrams. Methods, 34, 266–272.
Chernov, A. A. (2003). Protein crystals and their growth. J. Struct. Biol. 142, 3–21.
Giegé, R., Drenth, J., Ducruix, A., McPherson, A. & Saenger, W. (1995). Crystallogenesis of biological macromolecules. Biological, microgravity, and other physico-chemical aspects. Prog. Cryst. Growth Charact. 30, 237–281.
McPherson, A. & Giegé, R. (2007). Crystallogenesis research for biology in the last two decades as seen from the international conferences on the crystallization of biological macromolecules. Cryst. Growth Des. 7, 2126–2133.
McPherson, A., Malkin, A. J. & Kuznetsov, Y. G. (1995). The science of macromolecular crystallization. Structure, 3, 759–768.
Rosenberger, F. (1996). Protein crystallization. J. Cryst. Growth, 166, 40–54.
Sauter, C., Lorber, B., Kern, D., Cavarelli, J., Moras, D. & Giegé, R. (1999). Crystallogenesis studies on yeast aspartyl-tRNA synthetase: use of phase diagram to improve crystal quality. Acta Cryst. D55, 149–156.


text: How to build something cool My general workflow for building something cool Uncategorized Run into a problem anytime, anywhere Understand why this problem happened Identify what the solution to the problem would be Write it down and mull on it for a while until something seems about right Really explore the solutions to the problem Draw out a process to best solve the problem Translate that process into something interactive Ask other people if that's how they'd solve the problem If it's not, iterate until more people find that solution the most comfortable to them Once it's accepted by a small user group, you can finally build the damn thing Well maybe not build it, but at least you can make a fairly convincing prototype. Ask more people if that's a good way to solve the problem If it's not iterate until more people find that solution the most comfortable to them Now you can build it You know how you compile every few lines of code? You also need to make some checkpoints to check with users that your build works. In a similar build-test-iterate loop as above, you end with a decent alpha product Test the hell out of it with as many people as you have identified as your problem space's user group Iterate, improving functionality before features The cool thing is finally built. Now go back to the beginning and have fun

subset: commoncrawl


text:
This article or section is a stub. You can help the Far Cry Wiki by expanding it.
|Bowa Seko
Sefapane is a location in Far Cry 2.
It is located in the north-western sector of the Bowa Seko district. This town is the first you arrive in after entering the Bowa Seko region. It is controlled by the friendly faction. Before the war, it was most likely a low class town with only a few residents, though with a decent amount of trade. It was probably the gateway between the Bowa Seko and Leboa-Sako provinces.
If you subvert one mission in Bowa Seko, one of the player's buddies and the player must kill everyone in this town after blowing up a garage. This is a good time to retrieve the diamond which is in this town.
- The town is a ceasefire zone.
- The town has a few accessible buildings, one of which is a church tower, this tower contains a diamond case and is also an excellent vantage point for sniping, should that be the choice of the player to carry out the assassination missions assigned to this location.
- During the garage sabotage corrupt mission, there's a hill at the eastern side of the town, that can also be an excellent sniping point to cover your buddy, however it is recommended that you go with a semi-automatic sniper rifle, like the AS50 or the Dragunov SVD, due to the great amount of enemies that will be present during the mission.
|The Far Cry 2 Locations
|Leboa - Sako
|Airfield · Cattle Xing · Claes Products · Cock-Fights · Fort · Fresh Fish · Goka Falls · Lumber · Mike's Bar · Mokuba · North Railyard · Oasis (North East) · Oasis (South East) · Occidental Growers Company · PetroSahel · Police Station · Private Property · Scrap Salvage · Shwasana · Slaughter House
|Bowa Seko
|Airfield · Bowaseko Brewing Co. · Crash Site · Dental Clinic · Dogon Village · Heart of Darkness · Jungle Bivouac · Landing Zone · M-S Pipeline · Marina · Mertens-Segolo Pipeline Company · Mosate Selao · Outpost · Polytechnic · Post Office · Prison · Rail Xing · Ranger Station · Sediko · Sefapane · Sepoko · Swamp · TaeMoCo Mine · Weelegol Village


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Separatists leaders Syed Ali Shah Geelani, Mirwaiz Umar Farooq and Yasin Malik, under the banner of Joint Resistance Leadership (JRL), will march to Lal Chowk in Srinagar to protest against Prime Minister Narendra Modi's impending visit to the state on May 19. The JRL has also called for a shutdown on May 21.
The decision to call for the protest march was taken after a meeting of the three top separatist leaders at Geelani's Hyderpora residence on Tuesday.
The separatist leaders claimed the government has turned Kashmir Valley, especially the four districts of south Kashmir including Shopian, Pulwama, Kulgam and Anantnag, into a battlefield.
"(But) he (Modi) would try to give an impression to the outside world, as well as the people of India, that people in Kashmir are living happily with India… The fact on the ground is that Kashmir is like hell," the JRL said.
"To register their protests against Prime Minister Narendra Modi's visit to state on May 19, the people should march towards the historic Lal Chowk (in Srinagar)," the separatist leaders said.
Modi will inaugurate the 330 MW Kishanganga hydroelectric project and attend a convocation at Sheri Kashmir University of Agricultural Sciences in Jammu during his visit.


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Return to Search → Asia Pacific Europe Latin America Eurasia & Africa North America
Senior Manager, Communications
The Coca-Cola Company
- Location: Sugar Land, TX - USA
- Post Date: 5/7/2019
- Job Type: FULL TIME
- RELOCATION PROVIDED: No
Job Description Summary
Location(s):United States of America
City/Cities:Sugar Land
Travel Required:00% - 25%
Relocation Provided:No
Job Posting End Date:
Job Description Summary:Why Join Coca-Cola North America?
It's an exciting time to work in The Coca-Cola Company's flagship market. We're accelerating our momentum as the fastest-growing large consumer goods company in North America by putting people at the heart of our business and everything we do - whether we're innovating to give consumers the drinks and packaging sizes they want, or building our eCommerce capabilities. People are our focus when we're collaborating with our diverse network of locally-connected bottling partners, and when we're returning every drop of water we use to communities and nature. And people - with the different backgrounds, skills and perspectives they bring to our workplace - are helping transform our business, one big idea at a time. We empower our employees to challenge the status quo, make bold recommendations, experiment and adapt, so we can grow together and make a great business even better.
The Senior Manager, Communications for the Minute Maid Business Unit is responsible for building mutually beneficial relationships internally and externally with media and other stakeholders to advance the reputation of Coca-Cola North America and its portfolio of juice, dairy and plant-based brands.
Key day-to-day responsibilities include:
- Develop image and reputation of Coca-Cola North America's family of juice, dairy and plant-based brands in the media and across all internal and external audiences with a specific focus on innovation.
- Develop internal communications tools and programs to support employee engagement within MMBU, as well as awareness of the unit's priorities throughout Coca-Cola North America.
- Build strong relationships with key contacts in brands, media, community, customers and bottlers in order to increase receptivity to the company's communications.
- Serve as company spokesperson to the media and other external audiences on critical issues and announcements.
- Provide ongoing subject matter expertise and consultancy services to MMBU management on the impact their business decisions have on key constituencies, including consumers, customers, bottlers, media, community leaders, employees and other constituents.
- Prepare MMBU management to serve as effective media spokespersons.
- Manage and oversee outside PR agency resources and related budgets.
- Work closely with third-party groups and trade associations to ensure coordinated communication on matters impacting the juice, dairy and plant-based beverage industry in the United States.
- Serve as a subject matter expert on juice, dairy and plant-based beverages within the company's PAC community.
- Manage measurement tools and tactics to evaluate the effectiveness of communications strategies.
- Serve as a strong ambassador for the company's brands in every interaction with outside stakeholders to effectively convey the company's message to relevant audiences.
Minimum Education Required:
- Bachelor's degree in related discipline
Minimum Experience Required:
- 8-10 years related experience with exposure in media relations, stakeholder/community engagement, and corporate/leadership communications.
Our Growth Culture:
One of the reasons our company continues to thrive after 130+ years is having a company culture that supports and rewards behaviors that lead to growth. Our "Growth Behaviors," as we call them, are ways of being and working that help to make us successful. Think about how you can bring this to life in your next role at Coca-Cola. ?
Keep seeking, never settle. Staying curious about what is outside, and two steps ahead inspires us to challenge the status quo. Having the courage to look and leap is the way we grow. Because asking "what if?" pushes us to the next level as people and as a company.
Make it happen. True empowerment is the result of taking responsibility. This means giving yourself permission to see it, say it and do it, and owning the outcomes. Because we move forward faster when we all take action.
Version 1.0, 2.0, 3.0
Push for progress, not perfection. There are very few overnight successes. Greatness is borne of many little victories (and failures). Share v 1.0, test it, and make it better. Then create the next version. Because the moment we think something is perfect, it will be obsolete.
Include, value and trust each other. We are smart alone but together we are genius. This means being inclusive, giving the benefit of the doubt and being responsible for each other. Because, for our company to thrive for the next 100+ years, smart isn't enough. We need genius.We are an Equal Opportunity Employer and do not discriminate against any employee or applicant for employment because of race, color, sex, age, national origin, religion, sexual orientation, gender identity and/or expression, status as a veteran, and basis of disability or any other federal, state or local protected class. Apply Back to Top


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Ranee A. Gunaratne
Ranee A. Gunaratne passed away peacefully in her Spring, TX home on Monday, August 21, 2017 at the age of 75. Ranee was born in Colombo, Sri Lanka to the late Albert and Hazel Fernando on October 19, 1941.
John R. Beard
John R. Beard passed away August 14, 2017. He is survived by his wife of 39 years, Christine Beard. John joined Aramco in 1970-71 as a consultant in the Dhahran middle school then was hired as an employee the following year.
F Herbert Neuman
F Herbert Neuman, Age 87, died Friday, August 11, 2017 at a Senior Care Home in Las Vegas, Nevada. Herb was born at home in Hartwick Township, Osceola County, Michigan October 6, 1929 to Fred and Vera Mae (Gitchell) Neuman. He had three brothers Bill, Andy, and James and two sisters, Marge Sneary and Geneva Benscoter.
Joan Ellen McCabe
Joan Ellen McCabe (nee Hoy) age 84, passed away peacefully August 8, 2017 surrounded by her family. Joan was born in Yonkers, NY on December 2, 1932. A 1950 graduate of Yonkers Roosevelt High School, she then attended The Washington School for Secretaries in New York City.
Truus Groneman-Le Poole
Surrounded by her immediate family, Truus Groneman-Le Poole passed away on July 29, in Delft, the Netherlands, at the age of 72 due to complications after a fall down the stairs at home.
Helen Vera Abate
Helen Vera Abate, 83, of Lake Charles, LA went home to be with her Lord on Wednesday, July 26, 2017, in her residence surrounded by her loving family. Helen was born on October 4, 1933, in Merchantville, NJ to George and Michalina Rybak Bendyna.
Susan Elizabeth Webster Slavin
Susan Elizabeth Webster Slavin, 76, died on Tuesday, 25th of July 2017, after a valiant fight against cancer and other serious ailments. She was surrounded by her family when she entered the Lord's arms.
Jim L. Dykes
Jim L. Dykes, 78 of Abilene, passed away on Thursday, July 20, 2017 in Abilene, TX. Visitation will be from 5:00PM to 7:00PM today at The Hamil Family Funeral Home. Funeral services will be held at 10:00AM Saturday, July 22, 2017, at The Hamil Family Funeral Home with James King of the Grace Church of Abilene officiating.
Richard Joseph Dick Bastien
Richard Joseph Dick Bastien passed at age 84 on July 18, 2017 at Jacksonville Community Hospice, surrounded by family. The son of Louise and Dr. August Bastien, he was born in Flushing, NY on November 25, 1932.
Richard Morris Henderson
Heaven's ranks swelled by one as Richard Morris Henderson passed from this life to his heavenly home. Richard, a lifelong resident of Pensacola, was born in Pensacola, Florida, on December 27, 1945 and went to his heavenly home on July 11, 2017.
Henry Philipp Lang
Henry Philipp Lang, born February 18, 1923, in Kaefertal, Germany, passed away peacefully on July 10, 2017, in Anchorage, Alaska, leaving behind his wife of almost 70 years, Theresia; son, Peter; grandchildren, Kimo, Ninja and Merlin; and great-grandchildren Celeste, Jayson, Faye and Tobias.
William Dayton Alexander
William Dayton Alexander, 98, died on July 3, 2017. William was a native of Englewood, NJ and a graduate of Columbia University. He was a 5-year WWII Army veteran who served honorably in the Air Forces, activated several Control Offices in the U.S. and Europe for Air Transport Command; Major USAF retired.


text: Hey guys! Years ago I had an old friend of mine (long past on now) tell me how to build a surefire way to make a fire when I'm in bad shape. We used to trap the rivers and every once in a blue moon one of us would take an unplanned dunking in ten degree weather. And I tell you, that's a bad deal when you take a swim in water that feels more like fire and when you get out you instantly start seizing up with cold. You need a really quick way to get that moist junk on the riverbank to burn NOW. His way was to always have a fire starter. His was built in a tuna can, but any container would work. You'd then fill that can with thick corrugated cardboard round and round in the inside. Then you'd put one bit of cardboard into a U shape coming out of the can and crossing the can so you have a place to put the match. Fill the can with lard and thoroughly soak the cardboard. These things would light easily in the cold with one match and burn for thirty or forty minutes at least. By that time any wood over the top has dried out and started. I never actually burned one in the driveway to time it. I've been working on making a better version of this. As you can imagine, the lard filled can was kind of clumsy and messy to use. So I just took a small plastic bottle (an old pill bottle) and filled it most the way with cedar sawdust and lard, then I put two lard soaked cotton balls on top. I'll try to light this with a ferro rod in a little while and see how long it burns. I have found in my own testing that the lard burns longer than Vaseline and starts just as easy. I'll update this when I burn it. Please, let me know what great little fire starters you use. I'm always itching for new knowledge, and you guys are loaded with great ideas!!


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Parkinson's Support Group
02:00 PM - 03:00 PM — For anyone with Parkinson's
Mayfield Central Presbyterian Church ~ 22 N. Main Street, Mayfield
Monthly support group meeting ~ Third Thursday of each month
Support, advocacy and education for anyone with Parkinson's or other neurological conditions, their caregivers and family members.
This month's topic: A special presentation on the various services available through Catholic Family Charities and the process for securing assistance.
Guest Speaker: Tracy Paradise, Care Navigator For Senior Caregivers & Support Services
FREE meeting ~ No enrollment necessary
For more information, call group leader Rev. Bonnie Orth at 518-661-6566, or see Support Groups


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Do you wonder how via pulsa can help you change your pulse to be money that is able to transfer to your bank account? If you have a lot of but don't use it, you can get money through it. Go to that site for more info! Mobile phones or mobile devices are one of the many telecommunication tools that are very important for people's lives. However, the problem that usually arises during its use is the bloated costs when cell phone users who do not use the telephone with careful planning. Therefore, there are several plans that can be applied to save cellular phone credit costs. Here are some tips to save cellular phone credit costs:
Use a cellular package
The first thing you can do to save access to telephone financing is to use a cellular package according to the ID number you are using. By using a cellular package, there are certain packages that can be enjoyed with a certain time limit, such as SMS packages directly with the phone. This way, you can save money and switch to other expenses. However, you need to check first whether the selected cellular package can be used for all ID numbers or not.
Use of an integrated Wifi call system
If you want to save more on the cost of a cell phone, there's no harm in using a social media application that is also equipped with telephone facilities, such as Whatsapp, BBM, Line, Skype, and other applications. You can use telephone facilities by first connecting a cellular profile with an internet or Wifi signal. In this way, you don't need to waste a lot of credit to make calls to different telephone numbers with your network. However, this one system also has few obstacles when your location is not equipped with unlimited internet or 3G signal networks. Therefore, to overcome this, make sure the signal that is on your mobile phone is full and make sure you are in a location that is not blocked by buildings or high plants which generally can inhibit signal distribution.


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Who Uses This Software?
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Average Ratings11 Reviews
Ease of Use
Customer Service
Product Details
Starting PriceNot provided by vendor
Free TrialYes , get a free trial
DeploymentCloud, SaaS, Web
Installed - Mac
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Mobile - Android Native
Mobile - iOS Native
Live Online
In Person
SupportBusiness Hours
24/7 (Live Rep)
Vendor Details
- Auditus
- www.auditus.com
- Founded 2016
- United Kingdom
About Auditus
Auditus compliance software is built for organisations who perform health and safety assessments, compliance checks or quality assurance tests. A multi-lingual application, available online and offline on multiple platforms. Its state-of-the-art scoring system, risk alerts and powerful real-time reporting gives full visibility of your business' performance.
Auditus Features
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Cons: Had to purchase an iPad to run the software
Overall: I previously worked with a system that required hand writing audit points down & transferring information onto an online sever - which was so time consuming & repetitive.
Auditus enables audits to be carried out & recorded on my tablet, taking relative photos - providing valuable visual information to the clients - final audit scores can be provided at the end of the audit - removing potential stress to site operators awaiting results.
Overall - I would highly recommend Auditus - as it has definitely made my life both easier & more productive - Thank You!!
It's so easy to use!!!
Likely Extremely
Pros: I have used the software for a few years now and love the offline and online abilities it brings. It's easy to take photos in the app and link them to noncompliance's so the clients can see the issues at their sites.
Cons: As far as I can tell there are no downsides to using this software for my business. It gives me all the tools I need to do my job!
iPad app is amazing
Likely Extremely
Pros: its dashboards and reporting capabilities are amazing, on top of that its online system is multilingual. that makes it very easy for our european staff to navigate through the system.
Most importantly it has an app that works without internet connection.
Cons: Although system is very easy to use, but there is no user reference material.
Overall: it is very easy to use. For every user it shows very relevant information. we can control who can see what. its ability to send automatic reports on weekly basis makes it more easy for us to manage our sites.
Great system
Likely Extremely
Pros: Easy to use system, straightforward and can locate previous reports easily
Cons: Nothing to note ...have had no issues with the system
Overall: Use it daily with no issues
Likely Extremely
Pros: Extremely user friendly. IT support is excellent.
Cons: Haven't found any! I find the offline app particularly useful when auditing sites with poor or no internet connection.
Overall: Extremely user friendly.
audit with Auditus
Likely Extremely
Pros: Easy to use. Very full. Can be used by multiple listeners.
Cons: Nothing. Everything is easy. Clear and useful.
Overall: Our audit are finished before going out.
Review Of A Technophobe
Pros: Absolutely brilliant system. I find it really easy to use and would not hesitate to recommend it. The reports I type take a fraction of the time they used to due to the system being easy to understand and very user friendly.
Cons: I don't have any negatives. I can use it on my Apple Mac computer which is a complete plus (means I don't have to lug a big computer around)
I use the software to help with tenant inventories, property checks/audits and bond return disputes.
Pros: The software is very intuitive. I've found it very easy to build checklists and perform audits. Its reporting functionality is excellent and has really helped with our property management.
Cons: I'm struggling to find a negative. This software and app has really made a big difference to our business.
Quick and efficient
Likely Extremely
Pros: Clarity and ease of use
Works well in all situations
Cons: Occasional glitches
Overall: Effective auditing software
Easy to use and accessible
Pros: Pros- simplistic process and you can locate items with ease. use the software on a regular basis and it ie easy to navigate
Cons: Cons- I'm. struggling to find a negative. I use the software on a regular basis and it ie easy to navigate
Easy to use app for carrying out audits.
Likely Extremely
Pros: - Easy to use and is available off line.
Cons: Would like a drop down menu with standard phrases in for actions.
Overall: na


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This year, I really want to start running again. Before depression and injuries hit, I was able to run half marathons!
Today, I took the first step: I walked home from work on what was our first nice sunny spring day this year.
The walk was a brisk walk of thirty minutes, and a little less than 3 km. But it's the first step for many reasons.
1) My biggest issue is always just getting out there and doing it. I love it when I do get out, but that is always a problem because I build it up to this big mountainous task in my head.
2) I never even got out my bike last summer, let alone ran, so this is the opportunity to scope things out, find the best paths and be inspired by all the other runners I see along the way. I think exposure will be key.
3) I've been doing lots of yoga but no other exercise. And you really do need to walk before you run. This was a good walk, and it had hills too! So it's a good warm-up for my leg muscles.
Let's hope today's success inspires a second step! 🙂


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Volunteers wanted to assist with new art program
14 February 2019
The program aims to enhance the wellbeing of patients by providing them with a creative outlet and welcome distraction during their hospital stay.
St John of God Midland Public and Private Hospitals Coordinator of Volunteer Services Jenni Brown said volunteers will run on-the-ward activities from a mobile art trolley, engaging patients of varying abilities in a creative experience.
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Heifers go in. Steers go by. The calves were gathered from one place and trailed home to the corrals where we sorted them. This is the beginning of their separate lives… They both need to grow and gain weight, but … Continue reading
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With the new buyer it is important to note that your marketing efforts don't end once a new lead comes into your system – what we call Top of the Funnel (TOFU) marketing. Many companies do a good job at generating leads, but the problem is that most new leads are not ready to buy yet. And if a sales rep does engage and the lead isn't ready to talk with them, it reinforces the notion that marketing sourced leads are not great. As a result leads get lost, ignored, or snatched up by your competitors.
About the Author: Barry Feldman operates Feldman Creative and provides clients content marketing strategies that rock and creative that rolls. Barry has recently been named a Top 40 Digital Strategist by Online Marketing Institute and one of 25 Social Media Marketing Experts You Need to Know by LinkedIn. Visit Feldman Creative and his blog, The Point.
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InfusionSoft is a fully functional CRM and marketing solution created for small and medium size businesses. Comparing it to MailChimp or AWeber is like comparing a Lamborghini to a VW Golf. It provides highly advanced features for list segmentation, lead scoring and marketing automation. On top of all that, you can integrate all of these features with your optin form using Infusionsoft Tags + OptinMonster.
In dividing your list in this manner, you give yourself the ability to send more targeted communication. Some customers want both product and sales updates, while others might only want to hear about new versions. If you don't give them the chance to choose, you risk losing them all-together. Since customers make the best buyers, it's fairly obvious why you want to keep them subscribed to your customer email list.
First of all, BuzzFeed has awesome subject lines and preview text. They are always short and punchy -- which fits in perfectly with the rest of BuzzFeed's content. I especially love how the preview text will accompany the subject line. For example, if the subject line is a question, the preview text is the answer. Or if the subject line is a command (like the one below), the preview text seems like the next logical thought right after it:
Following the alignment process (Step #1), every sales rep should understand how to use the CRM and other lead intelligence tools to be able to quickly evaluate sales qualified leads and reach out to them quickly. Lead intelligence will help reps formulate a strategy for engaging with their prospects, gain their interest and trust, and develop a relationship that leads to a closed sale. This process is called sales nurturing. Every rep needs to update the CRM following every communication to keep lead status up-to-date and make sure that Marketing doesn't step on the sales process with inappropriate content. Once a sale is consummated, Customer Service should be notified via the CRM and take over managing the account. Sales also needs to set up automated reminders for following up with their customers and setting the table for up-sells and retained services. Marketing and Product Development also need to stay in the loop to send supporting, customer-centric content that educates customers on best practices and upcoming releases.
With the growth of the internet, the world has changed from one of information scarcity to one of information abundance. In fact, according to Google chairman Eric Schmidt "there was 5 Exabytes of information created between the dawn of civilization and 2003, but that much information is now created every two days and the pace is rapidly increasing".
InfusionSoft is a fully functional CRM and marketing solution created for small and medium size businesses. Comparing it to MailChimp or AWeber is like comparing a Lamborghini to a VW Golf. It provides highly advanced features for list segmentation, lead scoring and marketing automation. On top of all that, you can integrate all of these features with your optin form using Infusionsoft Tags + OptinMonster.
Use it to make sales on autopilot. Creating a sales funnel out of an email autoresponder sequence is a widely adopted strategy used by information marketers, but it can also be used by software companies, eCommerce businesses and service providers. For example, it could consist of a series of educational videos, a sales video and follow-ups to sell your information products. Or, you could create a sequence of free educational emails, and then invite them to a live or recorded webinar where you make an offer. For eCommerce businesses, your sales sequence could include promo offers for products your subscriber has just viewed on your website.
Beyond incentives, often a key part of the conversion funnel that can be optimized is the lead form. Although it is beneficial from a business standpoint to collect as much information as possible about a prospect, more forms fields leads to a reduction in form submissions. Experiment with different form lengths to see what is optimal in terms of lead capture and lead information.
Frequency matters, and how often you send emails can have a significant impact on your revenue and email engagement (and unsubscribe) rates. Send too much and subscribers can suffer email fatigue causing them to disengage and unsubscribe. Send too few and you lose the attention of your audience. They may even forget why they signed up leading them to unsubscribe.
The focus for marketers now is on being in the right place, at the right time, with the right calls to action, in order to capitalize on opportunities to build relationships with these knowledge-empowered consumers, and to give them what they want when they want it. As a rule, marketers recognize this evolution of the lead generation process, but many are faced with the challenge of how exactly to make that shift and execute effectively.
Yes, screens are getting smaller. And yes, an ever-expanding percentage of email is being consumed on mobile devices. Understood. But all that said, readers are entirely accustomed to scrolling and will appreciate a tidy and uncluttered look. Use headers, bulleted lists where appropriate, captions, and any other tactics to help go easy on the eyes and communicate effectively with the skimmer.
Engage your email readers with content that is short and to the point. Wordiness and fluff have no place in email marketing campaigns. Reveal your purpose up front and talk about how your purpose relates to the customer to get a connection started. Choose large fonts in an easy-to-read style. Your readers may be people who constantly read while they're on the go or they may have poor eyesight. You'll want them to be able to read and scan the email quickly.
Using content blocks allows you much more flexibility in the look and feel of your email. With a drag-and-drop editor, you are locked into content fitting into a certain section within a pre-defined template. This approach offers very little flexibility in changing the overall design or layout of the email. With content blocks, you have the freedom to make more changes to the layout and individual design components. For example, you can change the amount of padding or white space between the content blocks. You can also change the individual background color of a particular content block row.
An email marketing strategy is part of your overall marketing strategy and business plan. It helps you market your products and services with the use of the email channel with the best chances for making a profit and reaching your goals. That is because an effective email marketing strategy takes into consideration what your target customers are, their preferences and benefits they are looking for as well as your products services and industry and which email marketing messages are most effective. http://www.mailigen.com/blog/wp-content/uploads/2017/01/guest-blog-feature-1.2.32.png
And that leads us right into understanding service pricing and packaging. The email marketing services we reviewed range from about $5 per month to as much as $20 per month for a range of features. Many email marketing plans include unlimited email sends each month and bill you based on the number of subscribers. If you have a small list, then look for a company that offers a free plan, a low-cost plan for several hundred subscribers, or even a pay-as-you-go plan. On the flip side, many of these services also offer high-volume plans with up to 100,000 or more contacts. Sometimes this requires a custom plan that has to be arranged directly with a sales rep. If you're willing to commit, then look for the companies that offer discounts if you pay yearly rather than monthly. A few offer also money-back guarantees.
Marketing Automation is now a mature marketing technique, but across businesses many of the available tools are not fully exploited. Learn how to improve your marketing across the customer lifecycle by successfully leveraging B2B and B2C marketing automation techniques. Through B2B and B2C business cases, you will learn: how to review their current marketing automation maturity, how to prioritise their future investments in the year ahead, how to map their current approach against different customer touchpoints to identify new techniques.
While inbound marketing is getting a lot of buzz, a well-rounded marketing mix should include both inbound and outbound marketing strategies. Inbound works for broad lead generation activities, but outbound is good to amplify your inbound efforts, and target specific opportunities. So what exactly is outbound marketing? It's using outbound channels to introduce your message and content to your prospects, typically through rented attention, rather than making your content and messages availableon your own properties.
Cost per click advertising (e.g. AdWords, Yahoo! Search Marketing) overcomes this problem by charging advertisers only when the consumer clicks on the advertisement. However, due to increased competition, search keywords have become very expensive. A 2007 Doubleclick Performics Search trends report shows that there were nearly six times as many keywords with a cost per click (CPC) of more than $1 in January 2007 than the prior year. The cost per keyword increased by 33% and the cost per click rose by as much as 55%.
From the moment we're born, humans begin processing the world visually. That's why using images and videos in advertising is the most effective way to capture attention and convey information. Hero shots, infographics, explainer videos, video case studies, and video testimonials all help your prospects understand the benefits of your offer and why they should claim it.
In marketing, lead generation is the initiation of consumer interest or enquiry into products or services of a business. Leads can be created for purposes such as list building, e-newsletter list acquisition or for sales leads. The methods for generating leads typically fall under the umbrella of advertising, but may also include non-paid sources such as organic search engine results or referrals from existing customers.[1] https://neilpatel.com/wp-content/uploads/2013/06/1.jpg
Virtual Assistants. "There are many virtual assistant companies and individuals available to help you with your seller and buyer calls. I struggled through three of them and was ready to throw in the towel. Then I found an amazing company that trains well and is very well scripted and constantly improves. I hired them and have been with them almost six years with the same person. They will become an integral part of your team when utilized properly. They all charge differently. I personally spend between $125-$135 per week for six to eight hours of calling. This produces approximately 12-18 leads weekly for me so two to three per hour counting the sourcing time they have to do. They send me on a daily basis fully filled out property information sheets. This assures you that you're only speaking with quality leads that want to speak with you."
In B2B, inbound is the preferred channel of lead generation. The whole process of drawing a lead into doing business with you—by educating first and selling later—matches the B2B business model, where businesses don't make impulsive purchase decisions. Which is why inbound marketing in B2B takes leads through three levels of the sales funnel: ToFu (top of the funnel), MoFu (middle of the funnel), and Bofu (bottom of the funnel).


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Our fans' panel have their say on Leeds United's 4-1 defeat at West Bromwich Albion.
Well, that wasn't in the script was it? Leeds have finally paid the price for a failing that has been apparent for weeks; we've been dominating the possession during our games without actually converting that into clear cut chances and goals.
We had almost three times the amount of ball that the Albion had and yet they managed 20 shots at goal compared to only 15 by Leeds. When they got their chances they were ruthless in putting them away too, with four different strikers each getting on the scoresheet – Hal Robson-Kanu, Matt Phillips, Harvey Barnes and Dwight Gayle. At the other end Leeds typically blasted theirs wide or high of the target.
This needs to be a wake-up call for Leeds, we have to find a way to make our possession of the ball count as we did in those early season games. The decision to leave out Samuel Saiz seemed to make sense at the time as he was being wasteful of that possession Marcelo Bielsa craves so much, but without him we are then left bereft of those moments of Saiz magic which can create a goal from nothing, just as we saw when he came on late in this game. It's a dilema we need to resolve.
Man of the match: Pablo Hernandez.
Over the course of any season you're always going to get results like that, no matter who you are. If it was going to come against anyone, it was going to be the league's top scorers.
The Baggies set out with a game plan that Leeds couldn't handle from an early stage – overloading their own half and playing on the break.
It worked a treat and their pressure stopped Leeds from playing the eye-catching football we've come to expect.
In fact, the Baggies' high-intensity style was somehow reminiscent of how Marcelo Bielsa has set Leeds up this season.
Individual errors were rife across the board, but more worryingly, United didn't adapt and they couldn't find a way of playing through West Brom.
But, this was one game. It's the first time in 17 Championship games that they've been outplayed which is a testament to how good Leeds have been overall.
With the international break now upon us, Bielsa and his squad have an opportunity to regroup.
Man of the match: Pablo Hernandez.
West Brom provided us with our biggest test of the season so far and it was a test that we failed in spectacular fashion.
There was nothing particularly unusual about United's performance, perhaps we were a bit sluggish and a bit sloppier than normal with our passing, but we saw plenty of the ball, got into some very good attacking positions and struggled to turn those moments into attempts on goal. Just as we've come to expect.
West Brom showed strength, speed and opportunism to break on United time and time again and were more ruthless in front of goal than we have been all season. There should be no panic or knee-jerk reaction to this result. We won't face a team like this every week. Our two centre-backs, Kalvin Phillips and Adam Forshaw all come away with credit but Pablo Hernandez again gets my vote for man of the match. At 3-0, a swathe of United fans desserted the away end to hit the motorway home but, once they were out of the way, the remaining crowd got behind the team and Pablo rewarded us with a consolation. This was a good game with an awful result.
Man of the match: Pablo Hernandez.
Leeds United crashed to their third defeat of the season as they were well beaten at the Hawthorns. The 4-1 reverse was the heaviest defeat of the season and was warranted because the hosts were much the better team and costly errors at the back (once again!) handed the opposition their goals.
An unchanged Leeds team were put on the back foot from the start and were lucky to not go behind when Matt Phillip's shot crashed against the bar. Leeds struggled to create anything during the first half. Far too many players looked off the pace and Sam Johnstone was virtually a bystander.
The second half will not be one that the Leeds players will look back on with much pride. West Bromwich tore the Leeds defence to pieces in a rampant second-period display. Hal Robson-Kanu, Phillips, Harvey Barnes and substitute Dwight Gayle got the goals. Pablo Hernandez's injury-time goal didn't detract from how good West Brom were and how badly Leeds played.
I've not seen Leeds perform so badly this season and Marcelo Bielsa's comment about not needing additions when the transfer window opens is well wide of the mark – the squad is not strong enough to compete at the top continually without them. The loss of Luke Ayling and Gaetano Berardi has had an impact and with both absent for a considerable time, a defender is desperately needed. Goodness knows what could happen if either Pontus Jansson or Liam Cooper were injured.
Leeds must 'lick their wounds' for a couple of weeks while the international break is on. A bad day at the office for sure!
Man of the match: Nobody stood out.
Once again, the Championship confounded us all with its sheer unpredictability.
The scene was set for Leeds United to profit from the results of their peers. On Friday night, Sheffield Wednesday had managed against all the odds to hold the Blades to a 0-0 draw. Earlier, Derby and Bristol City lost at home and Birmingham could only draw. Okay, Norwich and Middlesbrough won but Leeds still had their superior goal difference, didn't they?
Furthermore, West Brom were down on their luck and Leeds were on a high. Leeds played like a team who had started to believe their own publicity.
In the first half, there were a couple of signs of what was to come but the Whites finished quite strongly. Seven minutes after the hour, Hal Robson-Kanu and Matt Phillips had put the Baggies in the driving seat. Marcelo Bielsa threw all his chips across the green baize and United's gamblers tried to chase their losses with inevitable consequences. In betting parlance: 'When the fun stops...'.
For once the international break is welcome.
Man of the match: Adam Forshaw.
We must remain philosophical. Marcelo Bielsa will. He spoke this week of the beautiful game and his desire to play it whilst he is always at pains to insist that no style is more valid than any other.
West Brom played tough, aggressive and direct football. Leeds were always seeking the time and space to impress their style.
Caught on the counter-attack having weathered the early onslaught, the Whites were once more chasing the game. The first goal came as Leeds were looking dangerous and aggrieved that they are never awarded penalties. It is not in the Argentine's DNA to contain and the Baggies found freedom when Leeds pressed. It highlights the necessity once more of scoring first; scoring at all is helpful. There will be finger pointing by some. He could have done this, he could have done that, but the more thoughtful person would take it on the chin and decide whether Leeds are good enough to be promoted when the cauldron of the Hawthorns has simmered down.
Despite the score, there is enough evidence that this side will always be knocking at the door – unlocking it and forcing it open is what challenges them on their return from the international break. The philosophically minded will reflect that it is better to take a pasting in search of going top, rather than avoiding being bottom.
Man of the match: Not applicable.


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Cary Carpenter
Cary Duane Carpenter, 41, of West Point, Mississippi, passed away on September 7, 2018, at his home in New Orleans, Louisiana.
Cary was born August 7, 1977, in Memphis, TN, to Paul Stephen Carpenter and Teresa Leak Carpenter. He is survived by his parents, brother Tyler Carpenter of West Point, MS; his brother Lucas Carpenter and sister-in-law Kayla Carpenter, and their two children, his beloved nephews Levi and Kane, of Cumberland, MS.
Cary graduated from West Point High School in 1995 and received a Bachelor of Science Degree from the Mississippi State University Bagley College of Engineering in 2008. He worked as a Civil Engineer for the U.S. Army Corps of Engineers in New Orleans for the past ten years. Cary was a veteran, serving his country honorably with the MS Army National Guard from 1995 until 2007, and saw combat duty during the Iraq conflict, serving in support of Operation Iraqi Freedom. He was awarded several medals and service ribbons during his military career.
Cary loved adventure and was an avid sportsman, particular enjoying fishing, hunting with his mother, golf, and snow skiing. He loved entertaining and annually hosted dozens of friends, including many from outside the US, during Mardi Gras in his adopted home town. Cary loved life and made lifelong friends everywhere he went. He loved to travel and visited many European and South American countries. Cary was loved and will be missed by friends that claim him as family from New York to Argentina.
Cary is preceded in death by his grandparents, Robert Joseph Leak, Dorothy Cole Leak, Irby Watkins Carpenter, and Beth Lafever Carpenter.
Services will be Monday, September 17th; visitation from 1:00 to 3:00 p.m. and funeral service at 3:00 p.m. at Holly Springs Funeral Home with Dr. Ken Hester of First Baptist Church of Pontotoc officiating, followed by a Military Graveside Service at Hillcrest Cemetery in Holly Springs, MS.
Pallbearers are Keith, Kenny, and Kevin Ferguson; Nolan Fulcher, Sandy Quinn, Chad Fitch, Tripp McCullar, and Wayne Carpenter, with honorary pallbearers, Casey McCullar, Joseph Carpenter, and Alexandria Hurdle.
In lieu of flowers donations can be made to: NOLA VFW Alfred E. Flynn VFW Post 8973. 531 Lyons Street, New Orleans, LA 70115
Holly Springs Funeral Home, Inc. | 1160 Highway 311, Holly Springs, Mississippi 38635 | 662 252-1161 | contact us
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posted in: denture stabilization | 0
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Civil Engineer
- Recruiter
- Conrad Consulting
- Location
- Derby, Derbyshire
- Salary
- £28000 - £33000 per annum + Benefits
- Posted
- 13 Oct 2018
- Closes
- 12 Nov 2018
- Ref
- Contact
- Matthew Thomas
- Sector
- Residential
- Function
- Civil Infrastructure Engineer
- Contract Type
- Permanent
- Hours
- Full Time
If you're a Civil Engineer with 3-5 years experience then this could be the opportunity you've been looking for.
A top client in Derby are seeking a Civil Engineer to assist with the design of SuDs drainage schemes and highways works using Microdrainage and AutoCAD.
The client works for clients throughout the UK and is recognised as one of the East Midlands' leading Engineering firms.
The firm will provide on the job training to ensure you can excel to the best of your professional potential. Client care is of paramount importance to the client meaning they are looking for an able communicator and someone who enjoys liaising directly with clients.
Specific experience in the use of AutoCAD will need to be demonstrated at interview.
If you're interested but not want to find out more before applying, then please call Matt Thomas at Conrad Consulting.


text: Jim Mcgrath of Quantum said: > Drive ASICs today are limited by pinouts more often that gate counts. > This means that every pin added has the potential to increase ASIC cost. Absolutely true. > But why use anything other than single ended for the other lines? > They should work find at the slow speeds required, and at lengths at > least good to 6 m (maybe longer). I tend to agree with that point, too. Since we are only speeding up the data transfer phases, BSY, SEL, RST, MSG, C/D, I/O, ATN pins should work fine. And depending on the implementation (I've seen some weird and wicked ones before), differential handshaking like arbitration and selection, not to mention SCAM, can make things complicated. On the other hand, I think data lines and parity needs to be differential just as much as REQ/ACK. This is unfortunate, since there are 18 of these pins, but i) Data lines are moving at at least half the data rate, i.e., presumably 20MHz for rumored Fast-40. This is just as fast as Fast-20 REQ/ACK today, and if we want to extend the cable length any more than what Fast-20 single ended specifies, we will need to do something about it. ii) Data lines must keep the setup and hold timing relationship with REQ/ACK. If we drive it through different flavor of drivers at that kind of frequency, it is difficult to guarantee that margin. For that reason, I would propose to specify differential drivers for REQ, ACK, Data and Parity lines, and single ended for everything else. ========================================================================= Tak Asami _|_|_| asami at dt.wdc.com _|/ _| WESTERN DIGITAL (714) 932-7621 : Voice _|_|_| (714) 932-6496 : Fax


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Event Information
Date and Time
Refund Policy
Refunds up to 1 day before event
Entrepreneurship 101
Make your own path.
This 5-week online course offers a mix of materials for every style of learning, weekly mentorship from expert practitioners and the tools you need to create your startup all in one place.
It's designed for emerging business leaders and innovators that want a masterclass in entrepreneurship.
Next cohort begins Monday 4 February 2019
4 February – 11 March
What you'll get
Over 30 video lessons from top entrepreneurs
1 project a week, for 5 weeks, and you can take as long as you want to finish the course
5 hours of learning a week fits around your schedule
Weekly live discussions
Access to the I2N E101 community on Slack
Continued help post-course
Week one
Start to define your startup idea. Craft your product problem statement and startup vision statement.
Week two
Develop your value proposition. Start to identify your customer and test your minimum viable product.
Week three
Understand your target customer and the size of your market. Start to build out your brand.
Week four
Learn the fundamentals of financial planning for your startup. Understand the different grants and funding programs out there for your startup.
Week five
Delve into the legal foundations you will need to know to start your company.
What you'll learn
• Develop your startup idea, product vision and value proposition
• Create the Minimum Viable Product (MVP) to validate your product or service
• Learn and work with proven methods for startup formation
• Develop the financial model for your product
• Work efficiently on testing and validating your product with future customers
You'll make real progress in 5 weeks.
This course is practical, unlike other programs we focus on tactics that (if done right) will accelerate the growth of your company. With a carefully curated a mix of perspectives – you're not learning business, you're learning to build a startup with proven methods.
We're here to support you.
Weekly live sessions and access to our online Slack community of entrepreneurs from around the world, learning to create companies together. While you can learn at your own pace, we'll be here at I2N to guide you each week.
The world around us is changing fast.
This isn't your average course on entrepreneurship. Developed by MaRS Discovery District in Toronto, Canada, the E101 program has connected experts with deep experience from many fields – across sectors and specialisations - to help you see the patterns and opportunities where others see chaos.
Let's advance the world together.
MaRS has accelerated the growth of thousands of young companies since launching the E101 program in 2016 whose technologies and solutions meaningfully improve lives every day. This program is now available in the Hunter region (and beyond) to help entrepreneurs realise their potential.
What is I2N?
The University of Newcastle's (UON) Integrated Innovation Network (I2N) links innovation hubs with programming designed to support and develop innovation, entrepreneurship and business development across the Hunter region. The hubs within the I2N include Hunter Street, Williamtown and Muswellbrook.
What is MaRS?
MaRS is the world's largest urban innovation hub, located in the heart of Canada's largest and the world's most diverse city of Toronto. It's a launchpad for startups, a platform for researchers and a home to innovators. MaRS brings together educators, researchers, social scientists, entrepreneurs and business experts under one roof. Founded by civic leaders, the MaRS mission is equal parts public and private — an entrepreneurial venture designed to bridge the gap between what people need and what governments can provide.
Program Partner
UON is proud to be able to bring this program to you at a subsidised rate thanks to the NSW State Government's Boosting Business Innovation Program.
Date and Time
Refund Policy
Refunds up to 1 day before event