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China Looks Like a Bubble Economy, GMO Says
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China's cheap, it's underperformed other emerging markets and its economy has the wind at its back. What's not to like?
How about its bubble economy? That's what keeps GMO'sEdward Chancellor and Mike Monnelly underweight--and up at night, according to a new report.
Take China's rapid credit growth. In 2009, non-financial credit expanded by the equivalent of 45% of the nations 2008 GDP--and has needed an increasing amount of credit to keep growing. From 2007 to 2012, the ratio of credit to GDP has increased to 190%. That's faster than the credit booms of Japan in the 1980s or the U.S. during before the financial crisis. And the speed of growth, rather then the actual amount is often more problematic.
Other stats are equally astonishing. "Last year, Chinese banks' share of total lending fell to only 52% of total credit creation, down from 92% a decade ago," Chancellor and Monnelly write. "In the fourth quarter of 2012, nonbank lending accounted for an astonishing 60% of new credit issuance." The comparison to the U.S. before Lehman Brothers' collapse is apt, they argue.
Chancellor and Monnelly provide a handy list of credit-related worries:
• Excessive credit growth (combined with an epic real estate boom)
• Moral hazard (i.e., the very widespread belief that Beijing has underwritten all bank risk)
• Related-party lending (to local government infrastructure projects)
• Loan forbearance (aka "evergreening" of local government loans)
• De facto financial liberalization (which has accompanied the growth of the shadow banking system)
• Ponzi finance (i.e., the need for rising asset prices to validate wealth management products and trust loans)
• An increase in bank off-balance-sheet exposures (masking a rise in leverage)
• Duration mismatches and roll-over risk (owing to short wealth management product maturities)
• Contagion risk (posed by credit guarantee networks)
• Widespread financial fraud and corruption (from fake valuations on collateral to mis-selling of financial products)
That said, Chancellor and Monnelly have continued to buy Chinese stocks but they own less that they would if they we were not concerned about the bubbly aspects of China," they say.
The iShares FTSE China 25 Index (FXI) exchange-traded fund has gained 0.05% today and 3.1% so far this year, led by--you guessed it--China's bank stocks. The Global X China Financials ETF (CHIX) has returned 6.15% this year.


text: It's often the tiniest hearts that need the most help from the start. That's why Nemours pediatric heart experts specialize in early detection and repair of congenital heart defects. If your child's been diagnosed with transposition of the great arteries, which is when two of the most important arteries are inverted, we're here to ease your concerns, answer your questions, and give your child the best possible chance for a healthy future.

subset: commoncrawl


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Halfway through the summer my not-inexpensive shea butter outside shower soap began to disappear once it wore away to about three to four inches long. I was puzzled and found it mildly annoying and couldn't imagine why the soap continually went missing. Whatever was stealing the soap has developed a real taste for it because the critter can no longer contain its desire, now nightly chowing down even before it is small enough to cart away. I think it is a rat or perhaps a skunk. My husband wants to trap it, but I ask the question–what if it is a skunk and you do manage to capture, how are you going to remove it from the trap??
I've tried discouraging the little borrower by switching from lavender to lilac, verbena, honeysuckle, and gardenia; no matter, it savors them all. It would be fun to film the creature with an infrared camera, similar to the ones used by National Geographic photographers for filming lions and tigers at night. Does anyone have one I can borrow?


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If you vowed to stop smoking in 2013, Big Tobacco won't be caught off guard. The industry is quickly moving into the manufacturing and sale of electronic cigarettes, a business which Bonnie Herzog at Wells Fargo said brought in $400 million to $500 million in sales in 2012 and will "at least" double in 2013.
"We're actually predicting that consumption of e-cigs could surpass consumption of traditional cigarettes in the next decade," she said.


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Quick Search: Choose Model and Year, then enter search topic.
Page: 65
Entering the PIN
As soon as you have switched on the telephone
and inserted the SIM card, you are prompted to
enter the PIN (PIN = Personal Identification Number).
You will find the PIN in the documentation you received from your network provider.
If you enter the wrong PIN three times in a row, the
SIM card will be blocked. To unblock the card you
must enter the so-called PUK (Pin Unblocking
Key). Follow the instructions on the PCM display
for this.
If you enter the wrong PUK ten times in a row, the SIM card will be blocked. This block can only be removed by the network provider.
You can deactivate the PIN prompt after the device is switched. To find out more, please refer to the section "Switching the PIN prompt on and off" on page 94 as well as " "Using a stored PIN" on page 94".
Having a conversation
There is a hands-free microphone in the steering
column trim panel (sports car) or in the roof
control console (Cayenne) of the vehicle. This
means that you can conduct all telephone calls
without using a handset.
You control the volume using the left rotary knob .
The microphone is directed towards the driver to optimize the hands-free quality.
Conducting a call using the headset
The call can only be conducted using the
headset if this has previously been connected
to the PCM via Bluetooth®.
To find out more, please refer to the sections "Authorized Bluetooth devices" on page 92 and "Adding a new Bluetooth device" on page 93.
Conducting a call using the cordless handset
Temporarily muting the telephone microphone


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Snooth Editorial Score
Submit a Critic ScoreClose
|Merwin Liquors
|USD 17.99 $17.99 750ml
|Grand Vin Wine Merchants
|USD 18.94 $18.94 750ml
Sausal Winery Zinfandel Alexander Valley Family 2009
Editorial Review by Gregory Dal Piaz:
Creamy and lightly spicy oak accents add vanilla and toasted cinnamon Pop Tart notes to the fruit here, which shows red cherry and raspberry notes. Smooth and medium weight on entry, this shows a nice bed of tannin and good acidity supporting a nice balance of raspberry fruit and spicy oak notes. The fruit turns a bit blacker on the back end, which gives way to a slightly oak-dominated finish. This is fairly elegant and long, certainly not a bruiser though I do think it needs 12 -18 months to fully integrate. 87ptsAdd winemaker's notes
Just outside of Healdsburg on Hwy 128 in the heart of Alexander Valley you will find Sausal Winery, a small family owned winery that produces award winning wine. Owned by the Demostene Family, Sausal Winery consistently produces premium estate bottled Old Vine Zinfandels (Family Zinfandel, Private Reserve Zinfandel, and Century Vine Zinfandel), Estate Sangiovese, and Estate Cabernet at reasonable prices.
Editorial Reviews for Sausal Winery Zinfandel Alexander Valley Family
Creamy and lightly spicy oak accents add vanilla and toasted cinnamon Pop Tart notes to the fruit here, which shows red cherry and raspberry notes. Smooth and medium weight on entry, this shows a nice bed of tannin and good acidity supporting a nice balance of raspberry fruit and spicy oak notes. The fruit turns a bit blacker on the back end, which gives way to a slightly oak-dominated finish. This is fairly elegant and long, certainly not a bruiser though I do think it needs 12 -18 months to fully integrate. 87pts
Big dill, vanilla and candied red cherry on the nose, with a touch of caramel and some nuanced white pepper. Nice feel right upfront with good acids and supple tannins -- in fact, the acid gives a very appealing sour red cherry/raspberry tone to the fruit. The fruit shows excellent purity and, while it is a bit simple up front, it gains a nice touch of gentle wood spice on the long, juicy finish. Very bright and delicious! 91pts
50 Year old vines - A tight and precise slightly smoky roasted blackberry tone greets the nose, it's candied in a way but still has a clarity and freshness to it that is appealing. It's a little exotic with a nice balsamic note and a touch of roasted/medicinal herb quality that adds nice tension to the nose. Soft in the mouth with enough tannin to give the fruit volume but still allowing the wine to be supple. Nice juicy acids support rather fresh raspberry fruit with that same herbal/medicinal character contributing a bit of edginess to the wine and leading to a nice, slightly tarry and earthy finish. 89pts
Fumey on the nose at first with nice underlying sour red fruits and a bit of candied apple and red plum with a notes of white pepper and some soft animale notes and floral tones makes this different and fairly complex. Gains a tobacco/resin note with air. Rich, soft and gently sweet at first with a nice old vines feel. Good transparency to the flavors and for all the intensity there is no fat here. Very wild raspberry and plummy with nice purity to the fruit but not much else going on. Finishes on the short side with a sneaky return of red raspberry, vanilla, herb and resin. 89pts
Very slow to open with subtle vanilla and herb tinged dark, marmaladey fruits that recall date and fig. Fairly caramelly with faded baking spice tones. Big and plush on entry then turning more focused and elegant on the mid-palate. The boysenberry fruits in the mouth are orange tinged with the citrussy acids and the tannins offer a creamy mouthfeel but the wine feels diffuse and while there is a nice candied/medicinal edge to the finish it's a bit to short. Appealing mouthfeel but lacking in intensity. 85pts
Member Reviews for Sausal Winery Zinfandel Alexander Valley FamilyAdd your review
The wine is somewhat lighter in color, with a rich ruby color permeating the glass. The nose is classic Zin, with a rich combination of ripe fruit scents, from ripe blackberries, sweet cherries and plums, along with a characteristic sweet spice that practically dares you to drink in the whole glass. The taste is very fruit forward, but not really jammy as some Zins tend to be, it has very nice balance of fruit, spice and acidity. Midpalate, the fruit subsides and the spice really kicks in, carrying through to the wonderfully supple finish supported by lovely, soft tannins. See my full review on my blog here: http://allaloneonaplanetfullofpeople.blogspot.com/2013/02/2009-sausal-family-zinfandel.html
herbal aromas, little fruit noticed on the nose, a little bit of vanilla, coconut and maybe even dill. the palate is filled with dark fruit, medicinal, acidity prevalent, layers of dark fruit, oak, a classic "old school" zin from California
Very smooth on the nose, and not overpowering with Oak. The finish was very peppery, with hints of cherry and rose pedals. Perfectly paired with steak.
Spicy red rum cake nose. Flavors are quite deep and dark, oaked and leathery, but still managing to be juicy.
Figgy nose. Mellow, slightly barnyard flavor. Not too impressed.
Starts out as a hot wine. Strong tast of alcohol in the first sip. 20 minutes of air helps it big time. Fruit emerges--plums or blackberries--and alcohol diminishes. Very robust jammy wine--typical of the style of the day for old vine zins. It will be interesting to finish off the bottle, tonight. Last night we served this wine with grilled sirloin, stuffed potatoes, and spicy grilled cauliflower and grape tomatoes. $16.99 in 2009.
Stewed tomato and basil on the nose with apple, wholesome grainy mouth with a long finish.
External Reviews for Sausal Winery Zinfandel Alexander Valley Family
Winemaker's Notes - This estate Zinfandel is produced from dry farmed vines that average 50 years of age. Following fermentation, the wine is aged twenty months in a combination of American and French oak barrels, adding complexity. The result is a soft and approachable Zinfandel. Pair this wine with spicy pasta, pork or grilled chicken. The Family Zinfandel will cellar well for at least six years.
WE 92 Pts - This estate Zinfandel is produced from dry farmed 90-year-old vines. This wine is aged for seventeen months in French oak barrels, yielding a jammy and dense Zinfandel. The Private Reserve Zinfandel will cellar well for at least ten years.
Wine Tasting Notes
All Prices
Merwin Liquors
Minneapolis, MN (900 mi)
Sausal Zinfandel »
$17.99 750ml USD 17.99
Grand Vin Wine Merchants
Olympia, WA (2,400 mi)
$18.94 750ml USD 18.94
Food Pairings for Sausal Winery Zinfandel Alexander Valley Family
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$19.9917% offBanshee Mordecai 2012
$20.8922% offL'Ecole No. 41 Luminesce Seven Hills Vineyard 2012


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VenturingTrax Advancement Spreadsheets
From MeritBadgeDotOrg
The Trax Spreadsheets are a collection of FREE spreadsheets developed by Frank Steele of Troop 615, Coastal District, Bay Area Council of League City, TX. They are for use by Scouters to keep track of advancements, awards, training, and even popcorn sales. Most are available in versions for both Microsoft Excel and OpenOffice Calc.
The following Trax Spreadsheets are available for use at the Venturing level:
VenturerTrax: Bronze, Gold, and Silver Awards
SeaTrax: Sea Scout Rank Advancement
RangerTrax: Ranger Award
QuestTrax: Quest Award
TrustrTrax: TRUST Award
See also
|Venturing portal


text:
STRUTHERS, OHIO: A northeast Ohio man has been arrested and charged in the slaying of his ex-girlfriend�s 13-year-old son.
Police said 43-year-old Zaryl Bush of Struthers was arrested Tuesday, following the weekend death of the boy at a hospital. Authorities say they plan to file more charges on top of first-degree murder.
The (Youngstown) Vindicator said the investigation began last week when the boy suffered head injuries and frostbite on his feet. Police, who said they were tipped off by a relative, are waiting on autopsy reports to determine what exactly led to his death.
The boy�s mother � who has not cooperated with the investigation � hasn�t been charged. The county children�s services agency took custody of her other children.
Jail records did not say if Bush had an attorney.
Information from: The Vindicator, http://www.vindy.com


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Daniel Brooks wins the play-off for the Madeira Islands Open - Portugal - BPI with a four on the first extra hole to Scott Henry's five. Both players found the 18th green in two but Henry three putted from 25 feet right of the hole, with Brooks safely two putting from 12 feet for victory.
This website is © The PGA European Tour 1997 - 2019. PGA European Tour, Wentworth Drive, Virginia Water, Surrey, GU25 4LX. Registered in England No. 1867610.
All rights reserved. No reproduction, distribution or transmission of any information, part or parts of this website by any means whatsoever is permitted without the prior written permission of the PGA European Tour.


text:
Wednesday, July 6, 2011
What I Learned Wednesday: 7 July 2011
More luck at Italiangen.org: I found a Benedetto and Maria Davi who, based on calculated year of birth, appear to be the people I have identified as parents of Giovanna "Jeni" Davi, my husband's great-grandmother. I'll be sending off for death certificates within the next couple of weeks. The Davis I found died in 1934, so I'm hoping that the form will be as detailed as the 1907 one I received for Julius Koehl and that the informants knew who their parents were.
I did some bookmark cleanup and organization - added some links to my Texas links and some items to my Research toolboxes here and on Weebly. I have also started working up a list of resources on Diigo. What did I learn? Bookmarking and bookmark organization takes a lot of time! This needs to be one of my resolutions - do a bit of bookmark management each weekend before I start regular research.
This weekend I discovered the "U.S. Cemetery and Funeral Home Collection" on Ancestry.com. It gives the full text of the record plus extracted information. Pretty neat if one of the names you are researching shows up there - for the person I was researching, in addition to the full obituary, it provided names of parents, siblings, spouse, children, age at death, residence, date and place of birth, date of death, and military service in a convenient form for reference. I'll have to give it a whirl with some other names.
Strange item of the week:
Three Nora Lees? I have been working on the family of Minnie Brinlee and Raligh Jones. Their oldest daughter was Nora Lee, who married Charles Alexander Whitaker. Their oldest son, Cecil Odes, married a Nora Lee Small and another son, Burl, married a Nora Lee Scroggins. Or so say the entries on Findagrave.
4 comments:
Thanks for the hint about U.S. Cemetery...being on ancestry. Need to check it out. I hope I can find it.ReplyDelete
Like you, also had a person leave me a FAG message and I can't wait to share. I hope the maiden name helps in your research.
That's cool about the Cemetery database on Ancestry. I didn't know they had that. Will have to check out soon.ReplyDelete
I just found an entire family in one plot on findagrave. That was the good news. The bad news is that I was just in that cemetery two weeks ago and it is about 10 states away from me :-(ReplyDelete
Barbara - I checked out several different hits there - some have more detail than others, since they are taken from various source, but the one I used was really helpful.ReplyDelete
Tonia - I keep forgetting to regularly check Ancestry and Family Search for new databases - I should do that every week.
Debi - I hate it when that happens! But maybe you can use it as an excuse for a return visit?


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Holiday Inn Concord Downtown
Holiday Inn Concord Downtown
172 North Main Street
172 North Main Street
Concord, New Hampshire
United States
Check In: 3PM
Check Out: 11AM
Check-in Age: 21
Book With Us Advantage
- Best Price Guarantee
We promise you access to the guaranteed best available prices, or your first night is free.
- Online Reservation Guarantee
Your room is guaranteed.
- No Booking Fees!
We do not charge any booking fees for making reservations directly with us.
- Data Privacy and Site Security
IHG takes your privacy seriously and works to protect you. All personal information you provide is encrypted and secure.
Welcome to Holiday Inn Concord Downtown
Newly renovated guestrooms completed June 2016!
Situated in the heart of NH's Capitol, the Holiday Inn Downtown Concord is the only hotel located on Main Street. The charming, historic and newly redesigned downtown, with local restaurants, shops & pubs, is within walking distance. Business travelers will enjoy easy access to Interstate 93, Interstate 89 and Route 4; while families who want to experience New England will be pleased that we are only an hour away from ME, VT & MA. Not only is the hotel just steps from the Capitol Building & State Offices but it is also only 2 miles to Concord Hospital, St. Paul's School & NHTI; less than a mile to UNH School of Law & the Capitol Center for the Arts; and a short drive to the Outlets, NH Motor Speedway, Canterbury Shaker Village, Pats Peak & Gunstock. Our on-site restaurant, EJ's on Main, serves breakfast & dinner daily. Room Service is also available as an added benefit when you've had a long day! Complimentary amenities include high-speed wireless internet access throughout the hotel; parking; a 42" flat screen TV; and a fridge & microwave in all of our guestrooms. Relax in our indoor pool & hot tub; or use the fitness room to keep up with your workout routine.
Come and enjoy newly renovated guest rooms in one of the most convenient locations in Concord, NH.
Kids Stay and Eat Free
Kids ages 18 and under stay for free when sharing their parents' room. Up to four kids ages 11 and under eat free any time of the day in any Holiday Inn® on-site restaurant.
*View Terms & Conditions for Details.
McAuliffe-Shepard Discovery Center! Explore the 21st century interactive exhibits, state-of-the-art planetarium, cafe and science store. www.starhop.com or (603) 271-STAR (7827) for exhibit details, program schedule and ticket prices.Museum of NH History! NH Historical Society's museum offers exhibitions on the state's heritage and traditions and a variety of programs for children and adults. The museum store features an extensive selection of NH books, gifts and custom products.Chuckster's "Home of the World's Longest Mini Golf Hole!" and Home of Chuckster's Speedway! Plenty of fun for everyone: go-karts, mini golf, batting cages, climbing wall & jumpster! Outdoors - open seasonally. Food & 30 Flavors of Blake's Ice Cream too!
|Kids Stay Free
|Kids Eat Free
|United States, Canada, Central America, South America, Mexico, Caribbean
|18 and Under
|11 and Under
Free High-Speed Internet
Standard Wireless Internet service is included for all guests.
Shuttle/ Parking
- Car Parking Available
- Complimentary Daily Self-Parking is available
- We can not accept parking for 18 Wheeler Trucks (or any vehicle that takes up 2 or more spaces)
Pet Policy
- No pets allowed
- Service Animals Allowed
- Pets Walking Area Available
- We are sorry, but due to our downtown location we do not allow pets in the hotel. However, there are several great kennels in close proximity to the hotel.
Credit Cards
- American Express
- Visa
- MasterCard
- Diners Club
- Carte Blanche
- Discover
- Smoke-Free Facility
- Number of Floors: 4
- Total Number of Rooms: 122
- Number of Suites: 1
- Number of Non-Smoking Rooms: 122
- Number of Accessible Rooms: 7
- Connecting Rooms Available
- Current Renovation: 2017 To 2018
- We have completed our interior renovations and have started painting the exterior of our building.
- Public Spaces Renovated in: 2018
- Guest Rooms Renovated in: 2016
- Public Entrance
- Guest Rooms
- Elevators
- Public Restrooms
- Registration Desk
- Exercise Facility
- Business Center
- Pool
- Number of Accessible Meeting Rooms: 5
Accessible Hotel Areas
- Registration Area
- Accessible Guest Rooms
- Business Center
- Meeting Room
- Exercise Facility
- Pool
Hotel Areas with Accessible Routes from Accessible Public Entrance
- Accessible Self-Parking
- Ramp Access
- Closed Caption TVs
- Visual Alarm/Alert in Public Spaces
- Communication Kits with Visual Alarms
Provided by Hotel


text:
Saunders' Slimmate clipboard combines a durable storage compartment with a solid writing surface to make it easier to make your work station mobile and to write anytime, anywhere.
The Slimmate clipboard is constructed of durable polypropylene and features a main compartment with a storage capacity of 3/4". The main compartment includes a high density foam insert that can fit and protect the iPad 2 and 3, as well as an easy access hole for use with the iPad's sleep/wake button.
The Slimmate's low profile clip can hold up to 1/2" of paper, while the main compartment is also built to store 8-1/2" * 12" sheets.
The low profile design of this clipboard enables it to comfortably fit into backpacks, briefcases, or anywhere space is tight. Take your work station with you where you go with the Saunders Slimmate Clipboard!
Special discounts may apply for high volume sales.
Contact our Bids & Quotes department for more information.


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Medical Aesthetics FACE® Apprenticeship
Medical Aesthetics FACE® Skin Care Associates of Central Texas
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Tattoo Reversal Procedures : All active soldiers seeking tattoo reversal of thier tattoos recieve a no charge for consults.
New Acaraya® Cosmeceuticcal Skin Care Line DERMFACE® specializing in dark pigmentation and excessive dry skin from medical aesthetic conditions such as psoriasis, eczxema, post-inflammatory pigmentation, polycystic ovarian acne conditions ( excessive facial hair growth )throughout the face. 1-888-769-3223.
Acaraya® Medical Aesthetics® cosmeceutical skin care line was created and formulated by Anna D. Rinehart, CIDESCO Diplomat through her 32 years of clinical experinece working with within the laboratory biochemists desiring to develop a comprehensive skin care treatment system that did not exxagerate its results. While spas, salons, med-spas and dermatologists can legally state this or that about thier products, Rinehart can substaniate the clinical effectiveness based on science and experience. " We want the community to always trust the name " Acaraya" ®, which means " teacher in ancient sankrit language. We embolden our clients with accurate information, education and direction and keep the market price low so we can reach a larger number of individuals that desrve healthy skin!
"No Gang-Tattoo 4 Me " is for any x-gang member that definitively seeks to change his/her life. We provide this service for our community at no costs.
Anna D. Rinehart, a woman that enjoys giving back to her community is evident through personalized skin care services which invite prayer as a part of the process.
Anna D. Rinehart , CIDESCO Diplomat, established and implemented the Medical Aesthetics FACE® presently is preparing for Yibin, China at the First Peoples Hospital, Medical Aesthetics Hospital for 2015.
Who is Anna D. Rinehart
Anna D. Rinehart, CIDESCO Diplomat, Licensed Aesthetician, Licensed CEU Provider, Licensed Facial Aesthetics Instructor, former Licensed Practical Nurse, Author: Fundamentals and Practice of Medical Aesthetics, author; Medical Aesthetics Clinical Skin Care Practice, Contributing author Dermascope Magazine since 1987, first CEU program developed @ Central Texas College in KIlleen, Texas, first Paramedical Aesthetics Program @ Scott and White Hospital in Temple, Texas through the Division of Plastic Surgery under Dr. Charles N. Verheyden, Chief of Plastic Surgery, first Medical Aesthetics books ever documented and established to teach physicians, nurses and aestheticians how to establish skin care programs that facilitate skin care health versus "facials" , medication and surgery. Rinehart served as the Coordinator for Medical Aesthetics @ the Sue Mayborn Womens Center for over 2 1/2 years in Killeen, Texas as well.
She developed Acaraya® Clinical Skin Care Products and has presented as an international speaker and educator for the Chinese Cosmetology & Medical Esthetics, Mexican Pan-American Esthetics, ( Guadalajara, Mexico). Developed and implemented a Medical Aesthetics practice under the Central Texas Clinical Skin Care Center in Harker Heights , Texas and Killeen, Texas. She developed Medical Aesthetics FACE® Facial Aesthetics Cosmetic Education for Austin, Texas, Corpus Christi, Texas and Dallas, Texas.Within these programs Anna D. Rinehart was able to attract physicians, nurses and aestheticians from around the world to learn how Medical Aesthetics as a clinical skin care practice could augment physician practices, promote skin care health as a speciality for nurses and aestheticians and ultimately benefit their communities by offering not a medication, not a new skin care product or pushing women to have surgery but rather giving women the tools needed to look thier best at any age. Anna D. Rinehart is the pioneer for Medical Aesthetics as a discipline utilizing a multidiscipline approach for health, beauty and medicine.
Mothers Teach Your Daughters Community Program
Outstanding community program developed to bring mothers and daughters together both for a bonding opportunity as well a an ability to learn how the skin functions as an organ, proper skin care techniques, as well as many variety of subjects that teach the mother and daughter to care for thier aesthetics now. Find out more through calling 1-888-769 -3223 .If, you would like to speak at one of these seminars then feel free to contact Anna D. Rinehart @ email@example.com or email@example.com.
Medical Aesthetics FACE® Apprenticeship Program
This program accepts physicians, nurses and aestheticians on a 3-5 day "40 hours/50 hours" "Hands-On" skin care skills development.
All applicants must submit an application/registration as a first step to secure their seat. Second , the licensed physician, nurse, and/or aesthetician should call 1-888-769-3223 to speak directly with Anna D. Rinehart, Director for the Medical Aesthetics FACE® Skin Care Associates of Central Texas in Harker Heights, Texas.


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Vibgyor Towers houses two premium luxury hotels and an exclusive club. The urban development authority plans to promote balanced development, with a few residential projects, international schools, convention centers and a luxury mall, along with the ever-growing premium commercial office spaces.
BKC is ideally located for easy accessibility from two major train termini, with equally good connectivity between the city and its suburbs. Bandra-Kurla Complex - Mumbai's most charismatic business district, located in the heart of the city - has emerged as a clear and uncontested solution for businesses looking to grow and succeed today.
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Marietta City Hall's roof leaks — News, Sports, Jobs, Ohio, Community Information
By Sam Shawver — The Marietta Times (email@example.com ). The Marietta Times
As plans continue for the renovation of Marietta's City Hall, one major concern that will have to be addressed first is the aging building's leaky roof.
"There are actually three roofs on this building front to back," said city safety-service director Jonathan Hupp.
He said a slate roof covers the front portion of the building, but the center and back sections of the roof are flat and have been covered with a combination of deteriorating foam and membrane materials that allow rain water to leak through.
Article Photos
Marietta safety-service director Jonathan Hupp, left, and city police detective Troy Hawkins are shown in the upstairs hallway of Marietta's City Hall where roof leaks left puddles and pieces of ceiling tile on the floor Wednesday afternoon.
SAM SHAWVER The Marietta Times
"Leaks are also developing in the roof over the fire department," Hupp said. "The roof has been patched and drains cleaned out, but it still leaks."
Puddles of standing water have stained the second floor's main hallway, just outside the former municipal courtroom. And buckets or trash cans have been strategically placed to catch water dripping into the police detectives' offices every time it rains.
"The main hallway stays wet, and some of the police records had to be moved out of a closet in our office and onto temporary shelving in a hallway because the closet ceiling has started leaking," said police computer specialist Rhett Walters.
Fact Box
About the
repair project
- Marietta's City Hall is in dire need of a new roof.
- The roofing job has to be completed before other renovations can be made to the building.
- The work could cost $100,000 to $130,000 to complete.
Source: City of Marietta
Wet ceiling tiles have fallen to the floor in several places on the second floor, exposing wiring and insulation.
Many of the leaks were evident long before the Marietta Municipal Court moved out of the city hall building in 2012 into a new location on the corner of Third and Butler streets.
In February city council approved hiring Davis Pickering Architects from Parkersburg, to begin preliminary design work toward a total renovation of city hall after the municipal court moved out.
But on Tuesday city engineer Joe Tucker said before any upgrades can be done inside the building, a new roof will have to be installed.
"Replacement of the roof has always been anticipated as part of the city hall work as water is infiltrating into the building," he told members of council's lands, buildings and parks committee.
Tucker said he wants to expedite the roof work, which could cost roughly between $100,000 and $130,000.
"The entire flat roof area has to be replaced," he said. "The current membrane and spray foam roofing is at least 15 years old. There is also a brick parapet around the roof with a capstone that's loose. And restoration work will also have to be done on some of the brick masonry."
In addition, Tucker said the roof does not have a proper pitch which prevents adequate drainage of rainwater.
"This will not be a simple re-roofing job, because we also need to seal the roof to prevent water from leaking inside the walls of the building," he said. "And core samples of the roof will have to be taken to determine which areas are made of concrete, metal decking, or other materials."
Tucker said as much work as possible would be done in-house for the roofing project, but the engineering department has a limited number of people and many other city projects to complete.
Engineering department project manager Jarrod Schultheisz said Fiber Tite Roofing of Wooster, the roofing supplier for the new municipal court building project, would be working with city engineers to help determine the scope of the project and develop roofing specifications.
"But this project has to be fast-tracked because of the deterioration caused by water leaking into the building," he said. "That water intrusion has to be stopped before any other work can be done."


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The decision by CBS to reverse its policy of refusing advocacy ads for the Super Bowl has erupted into controversy over the Tim Tebow antiabortion ad, which in turn touched off a meaning-of-feminism debate between Sarah Palin and the National Organization for Women.
Expect lots more where that came from.
For starters: An ad for ManCrunch, a gay dating service whose tagline is "Where many, many, many men come out to play," is now being reviewed for appropriateness by CBS. The ad features two men watching football who turn to each other and start making out, to the shock of a third buddy.
The combination of the CBS policy change (approved by the NFL), media companies desperate for cash, and last week's Supreme Court ruling on campaign advertising all but ensures that America's sports-watching experience is about to be inundated by political messaging, pop culture experts say.
Open door for advocacy groups?
"In agreeing to air the Focus on the Family's anti-abortion commercial with Florida quarterback Tim Tebow next Sunday, CBS has touched off a discussion on whether the relatively bliss-filled nature of sports television should be intruded upon by real life," writes Milton Kent on the FanHouse blog. "The ad opens the door for advocacy groups of all political stripes and causes to spread their messages to sports telecasts, which, by and large, have been devoid of that kind of controversy."
CBS took heat in 2004 for turning down a gay-friendly ad. The network recently said a statement that it "became apparent that our stance did not reflect public sentiment or industry norms." CBS says it is still reviewing ads for the Super Bowl broadcast and judging whether they're "appropriate for air."
But as with the gay dating service ad, CBS now ensures that its internal reviews will come under scrutiny. If the network airs an antiabortion ad meant to be inspiring – like the story of how Tebow's mom went against a doctor's suggestions to have an abortion in order to give birth to Tebow, a future Heisman Trophy winner – it'll be increasingly difficult for the network to turn down other "inspiring" messaging ads depicting other viewpoints.
A three-hour break from politics
TV networks have, of course, long aired advocacy ads, but the brouhaha over the Super Bowl broadcast is, in the end, largely cultural. It's the three hours when America collectively leaves behind the topsy-turvy world of American society and politics to focus on guys throwing footballs and to watch funny commercials filling up the multitude of timeouts and breaks.
"For it, against it, I don't care what you are," writes columnist Gregg Doyel at CBS Sports, bemoaning his employer's stance-change. "On Super Sunday, I don't care what I am. Feb. 7 is simply not the day to have that discussion."
Nevertheless, partisan politics and the economics of the media business are conspiring to change the feeling of game day, even beyond the Super Bowl.
After CBS's decision, which is being closely watched by the other networks, events like the Master's, the Olympics (200 million people could watch, NBC projects), and the Daytona 500 could all become targets of advocacy groups looking for a massive and rapt audience.
Last week's Citizens United ruling by the US Supreme Court – that corporations are entitled to individual free speech in the form of campaign spending – could also bring political ads to baseball, football, and basketball games during campaign seasons.
Not everyone thinks that's a bad idea.
"[Advocacy ads during the Super Bowl] aren't necessarily a bad thing," argues Robert Thompson, director of the Bleier Center for Television and Popular Culture at Syracuse University in New York. "One of the things about the Super Bowl is that the commercials are as interesting as the game, and this could go that way, as well."
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Property Line Adjustments
A property line adjustment procedure is used to relocate a single existing common property line between two abutting lots or lots of records. No additional lots or building sites may be created.
Property line adjustment applications must include surveys and written legal descriptions which have been prepared by a licensed surveyor in the State of Oregon. Chapter 33.667, Property Line Adjustments, outlines the zoning code requirements for all property line adjustments.
Applying for a Property Line Adjustment
A complete property line adjustment application submittal includes:
- Four (4) copies of the attached application form with original signatures of all property owners, the site legal description and other requested information. Original signatures for all property owners must be provided on one of the required copies of this form.
- Four (4) full size copies of a survey that is performed by a registered land surveyor. The property line to be relocated and the new property line must be clearly illustrated.
- Four (4) copies of the legal description for each adjusted lot and the area to be exchanged prepared by a surveyor in compliance with the "Information For Surveyors" listed below.
- One (1) full size copy of a survey showing existing development, including structures and paved areas.
- One (1) full size copy of a Site Utility Plan, including water, sanitary sewer and stormwater facilities.
- Application fee
Since property line adjustments move and reconfigure existing property lines, it is important to remember the following:
- A property line adjustment does not vacate a plat nor does it add lot lines or create a new lot. The number of lots after the property line adjustment may not exceed the number of lots before the property line adjustment. A land division is required to create an additional lot.
- A property line adjustment does not alter the availability of existing services to a site
- Property line adjustments can only be submitted on legal lots and lots of record (see definitions in Chapter 33.910 )
- The adjusted lots must meet zoning code requirements such as lot depth, width and area of the underlying base zone
- All development standards (e.g., setbacks, building coverage, minimum parking, etc.) are reviewed during the property line adjustment process
- Lots must meet all the standards of Chapter 33.667, Property Line Adjustments
- Off-street parking may not be eliminated or reduced below the code requirements
- The minimum required setbacks must be provided.
- Any necessary adjustments must be granted prior to approval of a property line adjustment
- No more than three property line adjustments on a site may be requested per calendar year
- Concurrent property line adjustments must be approved and recorded one at a time, in sequence
- The actual property line is not officially moved until a deed to transfer the title is recorded with the county in which the property is located
A property line adjustment may not be used to create:
- Street-frontage for a land-locked parcel
- A flag lot
- A lot that is in more than one base zone
- A lot that cannot meet the general development standards of Chapter 33.430, Environmental Overlay zones.
- A nonconforming situation
You may combine a lot confirmation with a property line adjustment to re-establish a previously platted property line and then move it.
- Property Line Adjustment Application - Complete this form to apply for a property line adjustment
- Property Line Adjustment Information - Information regarding the process of applying for a property line adjustment
- Land Use Review Fees
- Land Use Review Fees - Unincorporated Multnomah County
- Geeting Deed Information from Multnomah County
Questions? For further assistance with the land use and zoning regulations that apply to your site, please call us at (503) 823-7526, or visit Planning and Zoning Staff in the Development Services Center.


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Swimming Pool Blog
by Rob Cox, August 28, 2012
The Many Kinds of Pool Plugs
Swimming pool plugs come in all shapes, sizes and colors, and are used for many different purposes around the pool and for the pool equipment.
This simple blog post is a discussion on the various types of plugs used on swimming pools. Many you will be familiar with; others perhaps not.
Let's look at the many types of plugs and some of the uses of them.
1. Winterization Plugs
The most typical types of plugs used for winterization of pools is a tapered rubber plug with two stainless steel washers and a bolt passing through the center. Most commonly referred to as Expansion Plugs, because they expand as you tighten the stainless steel or nylon wing nut on top. Some pool guys call these Freeze plugs, although the more common use of that term refers to an engine block plug used for freeze protection on automobiles. These pool plugs are commonly available in 14 sizes, from 00 (fits 1/4in. holes) to 13 (fits 2-2.5in. holes).
Extended plugs are expansion plugs with an extra long bolt, to allow easier plugging of non-threaded pipes and pipes that are cut off at an angle, like the common 3/4in. return line in the wall. A Double Plug, used on old style Sylvan pools and some Anthony pools, is used for winterizing a combination skimmer, when the main drain pipe is tied into the pipe beneath the skimmer. These are also known as combination plugs.
Used as pipe plugs, to plug skimmers, returns, cleaner lines - to prevent water from entering the pipes during winter, expansion plugs have other uses too, such as pressure testing pool lines or other methods of leak detection, or stopping water flow from the pool when equipment repairs are made.
Expansion plugs can also be used to isolate certain lines, or abandon certain lines known to be leaking, until repairs can be made. They also come in handy to allow pressure to build-up and release in a pipe suspected to be clogged, creating a water hammer effect to help move debris stuck in skimmer lines.
Gizzmos are a combination plug used for skimmers. Long plastic tube that threads into 1.5in. and 2.0in. skimmers to serve as a plug to keep water out (recommended to wrap Gizzmo threads with Teflon tape). Their second purpose is to absorb ice expansion inside the skimmer, as rain water builds up inside the skimmer body and freezes during the winter, it can crack the skimmer body. The gizzmo breaks up the ice pack in the skimmer and collapses inward to absorb the strong force of expanding ice. Gizzmos come in two lengths, 12in. and 16in. and also have one called the Blow-Out gizzmo, that allows you to insert into the skimmer without lowering the pool water level, pop the top, and blow air through the one-way valve contained in the bottom, for the purpose of blowing the water out of the skimmer lines.
2. Filter Plugs
Different manufacturers use different plugs on their pool filters. Many don't use a plug at all - instead using a cap. The difference between a plug and a cap? Plugs have male threads, whereas Caps have female threads.
Pentair sand filters use a two stage plug - with their common 1/4in. Butterfly Plug, as I call it, to allow the filter to drain water for winterization or repairs. This 1/4in. plug has a small o-ring that is important to maintaining a proper seal, however 3 wraps of Teflon tape around the plug threads, in place of the o-ring will usually seal the plug. Remove the entire assembly from the tank by mistake, and you'll start to drain sand too. You may also have trouble getting the Spigot (as they call it) back in tightly, without cleaning the threads carefully and using silicone to improve the seal.
Most other sand filters use a plastic cap that threads onto a drain tube. A cap gasket or o-ring is used on these caps to make a seal. Missing the gasket? You can use silicone, wrapped over 3x with Teflon tape to substitute, in most cases. Filter drain caps vary in proportion to the drain tube they attach to, and can vary from a 3/4in. cap to a 2in. cap for larger sand filters.
D.E. and Cartridge pool filters most often use a 1.5in. or 2in. threaded plug to drain the tank. They can be positioned on the back of the lower tank body, or on a Tee fitting on the pipe that comes out of the bottom of the filter tank. Hayward SP1022C plug (1.5in. threaded) can be used as a replacement filter plug for many filters, with the exception of those filters using a 2in. threaded plug on their DE filters. Some filters use a tapered threaded plug as opposed to a gasketed plug, and with these, be careful not to overtighten the plug, which may cause the underdrain assembly or Tee fitting to crack.
Some DE or Cartridge filters don't have a drain plug at all, such as older Anthony or Wet Institute filters which can drain out of the push pull valve. Some homestyle plumbing jobs seem to have forgotten to install a Tee fitting into the underdrain assembly and you will usually find that a small hole has been drilled and tapped, with a 1/4in. plug used as the filter drain plug.
3. Pump Plugs
Almost all swimming pool pumps use a 1/4in. threaded plug, some with an o-ring and some without an o-ring. Those without the o-ring should use 3 wraps of Teflon tape to create a good seal. Old Teflon tape should be removed first; if years of Teflon tape builds up, this can increase the size of the plug too much, which may crack the pump housing. You can substitute most any kind of 1/4in. threaded plug for another, they are fairly universal, as long at they are NPT, or National Pipe Thread design. If your pump plug uses an o-ring however, you may have trouble getting a tight seal when using a 1/4in. plug without an o-ring.
Pool pumps usually have two drain plugs, one in the front, at the base of the hair & lint strainer or pump pot, and another plug further back towards the motor, to drain the volute, or impeller housing.
4. Heater Plugs
Pool heater plugs are most often 1/4in. plugs, and you will find one on both sides of the heat exchanger, or on the front header, where the pipes come in and out and on the rear return header. Cast iron headers or bronze headers use a 1/4in. brass plug or butterfly valve plug, whereas the modern thermoplastic headers will use a plastic plug, usually with an o-ring. Brass plugs should be lubed with green lube, like Aqua-Lube, to keep the rust from building up on the threads of the cast iron headers. These headers can be tapped, with a number 20-1/4in. tap, if internal rust becomes a problem.
5. Test Plugs
Test plugs look similar to winterizing plugs, except that they are not tapered, the sides are straight, and they have oversized wing nuts to allow for strong tightening. Some are smooth, some are ridged and some are inflatable. They are used primarily for pressure testing plumbing, because their straight sidewalls allow for a more secure plugging of the line; tapered plugs can push out of the line under high pressure. Another type of test plug has a hole that runs through the center of the plug, with a valve stem style threaded top. These plugs are used to push compressed air into the pipe, for the purpose of pressure testing or for blowing out the plumbing lines for winterization.
6. Hydrostatic Plugs
I recently wrote a blog post all about hydrostatic relief plugs. These are available in two types; spring loaded automatic and manual. Spring loaded plugs are referred to as hydrostatic relief 'valves' and are commonly inserted into the main drain pot, directly in the bottom center hole. The manual type of hydrostatic plug is commonly a Hayward SP1022B plug. Both manual and automatic hydrostats are meant to allow water from a high water table to enter the pool. This prevents 'floating' or 'popping' of the pool shell due to intense hydrostatic pressure, when an inground gunite pool is drained.
7. One-Way plugs
The Anderson Duck Plug or the Winter One-Way Plug are examples of plugs that are used during pool closing winterization. Air is blown in one direction through the plug, which pushes the water in the pipe out through the plug, and seals up to prevent water from reentering the pipe after the water is expelled. A plastic cap (not shown) is then pushed over the opening as added protection against water coming in.
Did I miss any pool plugs that you use? Leave a comment below.


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Since the Kids' Healthy Mouths campaign launched in August 2012, it has received tremendous media exposure through widespread TV, radio, print, outdoor and digital Public Service Advertisements (PSAs) in both English and Spanish. The campaign has been embraced by media outlets throughout the country, which have donated more than $33 million in free ad time and space. The PSAs aim to reduce the prevalence of dental decay by motivating parents to promote good oral health habits with their kids by reminding them to brush two minutes, twice a day to avoid oral pain in the future.
According to the study administered by the Ad Council:
Dental decay is currently the most common chronic childhood disease in the United States, affecting 16.5 million children. Oral disease disproportionately affects children from low-income families, who have nearly double the number of cases of untreated dental decay as the general public.
"We are extremely pleased with these results and with the overall impact of our campaign. Many dental problems can be avoided through simple changes in routines, and we're seeing now how receptive Americans are to this message," said Gary Price, Secretary and CEO of the Dental Trade Alliance Foundation. "Through our collective efforts together with the Ad Council, we have become the foremost voice on the issue of improving children's oral health nationwide."
"These are some of the strongest survey results we've seen after only one year of a campaign. This is the first campaign in the Ad Council's 71-year history to address oral health, and we are proud to be part of such an important effort to improve the lives of millions of parents and children," said Peggy Conlon, President and CEO of the Ad Council.
Since the campaign launch, there have been more than 1.3 million visitors to the campaign website, 2min2x.org, which offers a collection of free, two-minute videos — featuring notable characters from children's shows and networks, including Sesame Street and Cartoon Network— that kids can watch while brushing. The campaign has also benefited from tremendous press coverage, partnerships with influential parent-focused websites, endorsements from celebrity moms and strong support from the dental community.
The campaign has gone further in recent months — offering children's oral health tips to parents on their cell phones via SMS messages. Parents can subscribe by texting "BRUSH" to 30364 or by visiting the homepage of 2min2x.org. In the upcoming months, the campaign will launch a new mobile game app for kids and their parents and a new national in-school oral health education program that aims to reach lower income and minority children and their families. English and Spanish language brochures will also be distributed to dental offices this fall. All of the multimedia PSAs were created pro bono by ad agenciesGrey Group and Wing in New York.
Partnership for Healthy Mouths, Healthy Lives
The Partnership for Healthy Mouths, Healthy Lives (www.healthymouthshealthylives.org) is a coalition of the leading organizations in the field of oral health. The Partnership is committed to improve children's oral health so that they can develop into healthy, productive adults. The Coalition shares the view that no child should be in pain and suffer broader health issues or endure the social stigma and lack of opportunity resulting from untreated dental diseases and conditions. The Coalition's primary mission is to teach parents and caregivers, as well as the children themselves, to take control of their own health through oral disease prevention.
Members of the Partnership include: Academy of General Dentistry, Alliance of the American Dental Association, American Academy of Oral and Maxillofacial Pathology, American Academy of Pediatric Dentistry, American Academy of Pediatrics, American Academy of Periodontology, American Association for Dental Research, American Association of Endodontists, American Association of Oral and Maxillofacial Surgeons, American Association of Orthodontists, American Association of Public Health Dentistry, American Association of Women Dentists, American College of Prosthodontists, American Dental Assistants Association, American Dental Association, American Dental Education Association, American Dental Hygienists' Association, America's Dentists Care Foundation, Association of State & Territorial Dental Directors, California Dental Association, Children's Dental Health Project, Connecticut Coalition for Oral Health, Connecticut State Dental Association, Dental Trade Alliance Foundation, DentaQuest Foundation, Hispanic Dental Association, Maine Dental Access Coalition, Medicaid/SCHIP Dental Association, National Association of Dental Laboratories, National Children's Oral Health Foundation, National Dental Association, National Network for Oral Health Access, Oral Health America, Organization for Safety, Asepsis and Prevention, Santa Fe Group, the Society of American Indian Dentists and Washington State Dental Association.
Ad Council
The Ad Council is a private, non-profit organization with a rich history of marshaling volunteer talent from the advertising and media industries to deliver critical messages to the American public. Having produced literally thousands of PSA campaigns addressing the most pressing social issues of the day, the Ad Council has affected, and continues to affect, tremendous positive change by raising awareness, inspiring action and saving lives. To learn more about the Ad Council and its campaigns, visit www.adcouncil.org, like us on Facebook, follow us on Twitter or view our PSAs on YouTube.
Media Contact
The Ad Council
Lisa Cullen
Back to News


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Lost & Found
Police & Public Safety is the central repository for lost and found property on campus. If you have lost an item, please contact us. Items are kept on file for one semester before disposal. Identification or proof of ownership may be required for claiming property.
Police, Public Safety and Parking
Gannon Bldg, Room 2110
Phone: (517) 483-1800
Additional contact information »


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New bicycle map available
Bicyclists looking to explore bike trails across the state can get a new map showing all the trails in the state.
The map includes information on traffic volumes, shoulder widths of highways, bicycle facilities in communities, and safety tips.
Maps can be requested by emailing firstname.lastname@example.com or calling (785) 296-8593. For more information visit http://www.ksdot.org/bikeped.


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ESPN New York's Stephen A Smith reported yesterday on his show that the Orlando Magic inquired, the key word there, the New York Knicks about a potential Dwight Howard for Amar'e Stoudemire and Tyson Chandler trade. Given the contracts of Stoudemire and Chandler, the Knicks would have to also take back a sizable contract from Orlando. The 2011 figures in this rumored trade look like this:
Amar'e Stoudemire $18,217,705 million
Tyson Chandler $13,107,838 million
Total: $31,325,543 million
Dwight Howard: $17,885,400
The Knicks sit at $62,487,871 in salary. The Orlando Magic currently sit at $69,066,010 million dollars in salary. This figure puts them slightly under the $70 million luxury tax threshold. Here are the 2011 salaries for their players, significant to this trade, minus Dwight Howard:
Hedo Turkoglu: $10,600,000 million
Jameer Nelson $7,305,555 million
JJ Redick $6,500,000 million
Glen Davis $6,300,000 million
Jason Richardson $5,395,000 million
Chris Duhon $3,460,000 million
Quentin Richardson $2,446,200 million
Ryan Anderson $2,244,600 million
Earl Clark $1,200,000 million
Excluded: Von Wafer, Daniel Orton, DeAndre Liggins, Justin Harper, Larry Hughes
Because of the cap standings of both teams, both teams being under the luxury tax threshold, the Knicks and Magic have more flexibility with a potential trade. However, the Knicks swapping $31.3 million in salary with Orlando and taking in Howards $17.8 million in salary is not possible. Under the new CBA trade guidelines, the Knicks would have to take back another salary because swapping STAT and Chandler for Howard would not work. Here are some trade scenarios as pertained to each CBA rule:
- The Knicks take back 100% of Orlando's salaries plus 5 million. The Magic would have to give up $26,325,543 million or more in salary to make this scenario work. That means in addition to Howard's salary, the Magic would have to shed an additional $8,440,143 million in salary. Any trade of Howard likely will include the long term contract (3 years remaining, $34.2 mil) of Hedo Turkoglu. Turkoglu makes 10.6 million this season thus bring Orlando's salary price up to $28,485,400 mil. This trade will work under the new CBA rules. However, the Magic would be taking back an additional $2,840,143 million in salary. Under the 100% + 5 mil rule, the Magic would have to remain under the tax. Therfore they would have to give up another contract resulting in an excess of $1,906,153 million. In my opinion, ex-Knicks Chris Duhon (3 years remaining, approx $11 mil in salary) and Quentin Richardson (2 years remaining with an additional players option for 2013, approx 5 mil in salary, $7.8 mil with option) would be the most likely candidates to be included in a trade. I believe that the basic framework of a Turkoglu/Howard/additional contract for Stoudemire/Chandler trade is the most likely trade if one is to come to fruition.
- The Knicks take back 150% of Orlando's salaries plus $100k. The Magic would have to give up a little bit more than $20.8 million in salary to make this trade work. Given the long term contracts the Magic have on their roster along with the minor rebuilding they will need to do losing their star player, I believe the Magic would want to give up more than $20.8 million in a trade. In order to use this 100% + 100k clause, the Magic would have to give up between $20.8 million and $26.325 million (from the scenario above). However, if they were to give up Turkoglu, which they should and which they want to, they would eliminate this clause from being used in the CBA. This scenario would be plausible if the Knicks were going to take back any other Orlando player not named Hedo Turkoglu in a trade. This scenario is unlikely because I truly believe the Magic are going to part ways with Turkoglu as well as Howard in any trade they make.
- The Knicks and Magic never have serious discussions. Per the new CBA, Tyson Chandler, a newly acquired free agent, cannot be dealt until March 1st. If Orlando is swept off its feet by the Lakers, Clippers, Mavs, Nets or a mystery team, which I do not believe it will be, then they could move Howard before the Knicks can even discuss this trade seriously.
- The Knicks and Magic find a 3rd team. Conceivably, just like the trades that brought Carmelo Anthony and Tyson Chandler to New York, the Knicks could find a 3rd team to deal with. Any trade scenario involving 3 teams would have to include more parts than just Turkoglu/Howard and Stoudemire/Chandler. At this point it is impossible to predict what 3rd team could be involved because no serious talks have gone on between Orlando and New York.
In conclusion, the main idea of this long and potentially head-ache enabling blog post is that financially the Knicks could pull off a trade for Dwight Howard. The new CBA allows it and both teams have contracts to make it work. The factors that would hold up a trade go beyond the financials. Whether the Magic are fully committed to trading Howard is up in the air. It is unknown whether the Knicks would part ways with newly acquired Chandler. As well as whether to give up Chandler, there have been no indications from the organization that Dolan is ready to break his loyalty with Stoudemire. It also remains unknown as to whether Orlando would even take this kind of deal. They would be locked into two long term big contracts and two decaying Amar'e Stoudemire knees. In my opinion, I do not believe this is a trade that will ever come to fruition. I think it makes obvious sense for the Knicks but I think the financials of the situation would far outweigh the on-court benefits for the Magic. I believe Amar'e and Tyson Chandler could fit nicely with that already strong Orlando team but I do not think the two players would launch the Magic into championship contention. Either way, Chandler cannot be dealt until March 1st so the Knicks will have their current center for at least 6 more weeks before they go chasing after another team's star center. A lot can change in six weeks but if I had to bet a hypothetical house (seeming that I don't own a house) on whether this trade would happen, I would bet against it.Follow @tarmosino


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You can use DSCC to perform this task. For information, see Directory Service Control Center Interface and the DSCC online help.
Specify the minimum available disk space.
$ dpconf set-access-log-prop -h host -p port min-free-disk-space-size:memory-size
For example, to delete backup log files when the available disk space is less than 2 Mbytes, use this command:
$ dpconf set-access-log-prop -h host1 -p 1389 min-free-disk-space-size:2M


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Cornerback Tramon Williams and linebacker A.J. Hawk were both rewarded for their part in helping the Green Bay Packers reach Super Bowl XLV in early 2011. General manager Ted Thompson no doubt thought he was locking up two important cornerstones of his defense. Williams was an up-and-coming cornerback who was playing his best football toward the end of the season, while Hawk's limitations were seemingly offset by his supposedly invaluable quarterbacking of the defense.
As a result, Williams signed a five-year, $38.148 million deal on November 30th of last year. The deal contained $11.074 million guaranteed, including a $6 million signing bonus. The corner can also earn $1 million roster bonuses in years two through five of the deal. He is due $2.3 million in salary in 2012 and a $2.8 million roster bonus.
Hawk, meanwhile, was cut on the eve of free agency this past offseason, only to be resigned to a five-year, $33.75 million contract. The deal included an $8 million signing bonus. He is due $4.4 million in salary in 2012 and a $300,000 roster bonus.
Looking at both of their performances in 2011, it's hard to say Thompson is getting a good return on his investment so far.
Williams, of course, was never the same player we saw last year after injuring his shoulder in the opening game against the New Orleans Saints. Coupled with the suspect safety play he was playing alongside all year, and it's understandable that his game would slip a bit.
But time and again we saw Williams giving huge cushions to substandard wide receivers and simply using poor technique not only in coverage but when attempting to make a tackle.
Hawk, while at least his usual serviceable self early on in the season, was absolutely terrible after coming back from the calf injury he suffered on Thanksgiving in the game against the Detroit Lions.
What was troubling when it came to Hawk this season is how little fall-off there seemed to be when rookie D.J. Smith was asked to make the calls on defense. The conventional wisdom, and I admit I was one who thought exactly this, was that Hawk was the glue that held the defense together.
Not only did Smith hold the defense together, at least well enough for a rookie seeing his first action in the NFL, but he also added a playmaking element to the inside linebacker position that has been sorely lacking since the Packers switched to a 3-4 defense under Dom Capers.
Now, there is every chance that both Williams and Hawk, with a full offseason to heal, will come back and play at their 2010 levels in 2012. Thompson no doubt hopes that is the case, because right now he hasn't gotten much bang for his buck.
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There are aspects that I found would have been confusing if I hadn't read the books.
Book spoiler
Also the shaky cam for the action scenes was somewhat annoying but I imagine that's what kept it at a PG 13 rating instead of pushing it up to an R rating. Overall though its quite accurate to the books and the movie was very visually pleasing. Even though it was 2hours 20 min it still felt a bit rushed at some points but I can see why they had to cut it down. Still an extra 15-20 min would probably have been useful to make things much more clear to those who haven't read the books (and maybe extended the last action scene a bit since it felt very rushed).
For the record, I'm going to search this thread every few days. If I see the phrase "Battle Royale" in a post in which someone tries to cleverly draw a parallel between the two (aka the "I saw/read it back when it was called Battle Royale" comment), I'm banning them from High Culture for a few days. -ST


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Newberg VitaFelt Traveller HatHiking hat anthracite€179.00Tax incl., plus shipping
Delivery time: 2-3 Tagebrowngray
Newberg VitaFelt Traveller HatHiking hat anthracite€179.00Tax incl., plus shipping
Delivery time: 2-3 Tage
Only the finest of materials is used for the manufacturing of Stetson's premium headwear. Superior material choice results in excellent wearing properties, durability and a precious look and feel of the products. Natural materials such as soft cotton, cooling linen and delicate silk are breathable, lightweight and temperature regulating which makes the Madison flat cap an excellent companion on warm summer days. The natural UV-filter of these high-quality materials as well as the short peak of the Madison cap afford protection against the sun. Thanks to the light shades and classic patterns, the Madison cap can be mixed and match with all you summer outfits. Once the temperatures start to fall, you should swap your airy Stetson cap for a cosy winter cap. Warming Woolrich® wool, superior suede leather and soft cashmere gear up the Madison cap for the cold season. Elegant Harris Tweed, classic herringbone pattern or chequers make the Madison cap cosy inside and out. Stetson's Madison cap is a truly versatile piece of headwear that should not be neglected by confidently stylish gentleman.
You already found your headwear favourite in the Madison cap from the house of Stetson? Browse the wide range of Madison caps in different materials, colours and patterns and order online at Stetson.eu!


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Lyndon chose this as one of his favourite problems. It is accessible but needs some careful analysis of what is included and what is not. A systematic approach is really helpful.
Complete the following expressions so that each one gives a four digit number as the product of two two digit numbers and uses the digits 1 to 8 once and only once.
Take any prime number greater than 3 , square it and subtract one. Working on the building blocks will help you to explain what is special about your results.
Find the smallest numbers a, b, and c such that: a^2 = 2b^3 = 3c^5 What can you say about other solutions to this problem?
The flow chart requires two numbers, M and N. Select several values for M and try to establish what the flow chart does.
Show that it is rare for a ratio of ratios to be rational.
Take any pair of numbers, say 9 and 14. Take the larger number, fourteen, and count up in 14s. Then divide each of those values by the 9, and look at the remainders.
Make a line of green and a line of yellow rods so that the lines differ in length by one (a white rod)
When if ever do you get the right answer if you add two fractions by adding the numerators and adding the denominators?
An introduction to the ideas of public key cryptography using small numbers to explain the process. In practice the numbers used are too large to factorise in a reasonable time.
How many divisors does factorial n (n!) have?
Think of any three-digit number. Repeat the digits. The 6-digit number that you end up with is divisible by 91. Is this a coincidence?
6! = 6 x 5 x 4 x 3 x 2 x 1. The highest power of 2 that divides exactly into 6! is 4 since (6!) / (2^4 ) = 45. What is the highest power of two that divides exactly into 100!?
Great Granddad is very proud of his telegram from the Queen congratulating him on his hundredth birthday and he has friends who are even older than he is... When was he born?
In how many ways can the number 1 000 000 be expressed as the product of three positive integers?
Find the highest power of 11 that will divide into 1000! exactly.
The number 12 = 2^2 � 3 has 6 factors. What is the smallest natural number with exactly 36 factors?
A number N is divisible by 10, 90, 98 and 882 but it is NOT divisible by 50 or 270 or 686 or 1764. It is also known that N is a factor of 9261000. What is N?
Find the smallest positive integer N such that N/2 is a perfect cube, N/3 is a perfect fifth power and N/5 is a perfect seventh power.
The sum of the cubes of two numbers is 7163. What are these numbers?


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Sale on canvas prints! Use code ABCXYZ at checkout for a special discount!
Lourry Legarde sold a 12.00" x 12.00" print of Icy Passion to a buyer from Irvine, CA.
Lourry Legarde is a talented artist whose work encompasses a variety of mediums, from traditional oils to photography and digital paintings. Glowing with vibrant color and rich hues, her creations ri...
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Shirosaki Black
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congratulations on your sale :D,beautiful piece of art.love your abstract
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Main Content:
Maternity Tees Sale
Shop Discounted Maternity T-Shirts at Old Navy Online
Seize the opportunity to save at the Old Navy maternity tops sale. Our maternity tees sale includes the latest fashions in a variety of bright colors. We offer maternity tops that are comfortable and have a great fit and it's a bonus that they are so flattering. Save on maternity tees in this collection that are made to expand as you grow so that you can wear them for your entire pregnancy and beyond. These tops are perfect to wear with pants or shorts and look fabulous with a jacket. Find tees in solid colors, stripes and prints that will complement your wardrobe. You will love the style of these shirts and the price can't be beat.


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Rain started in GriggsDakota just as blooming began in the Shelter belt.
This is a row of Juneberries planted by USDA Natural Resource Conservation Service in 1999 which contains more than one variety.
The shorter Juneberry bushes are just beginning to bloom in earnest yet. Click to find the answers to many questions on these delicious berries that are referred to as Saskatoons in other parts of the world including Canada.
After years of believing that the taller trees in this row were another juneberry variety, I have been told by an expert that they are apricot trees. There has never been an apricot on them, but they blossom beautifully.
Picking and freezing juneberries takes place around the Fourth of July in GriggsDakota.
Picking and freezing juneberries takes place around the Fourth of July in GriggsDakota.
Birds, deer, and the weather work against us as we coax the blooms into fruit suitable for pies, jelly, and other goodies. We need the rain, so for now we will enjoy the blossoms as they arrive and wait.


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Careers With LeafGuard
There are not any job openings at this time.
There are not any job openings at this time.
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LeafGuard Brand by Englert is the only patented, one-piece, seamless gutter system, that is guaranteed not to clog for as long as you own your home, or we will clean your gutters for free.
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- Read the latest issue of the ProbesOnline E-Newsletter.
In This Issue
Bring the Power of Fluorescence Quantitation to Your Bench — The Qubit® 2.0 Fluorometer
Reliable Cell Viability Data in Only 10 Minutes — PrestoBlue™ Cell Viability Reagent
Simple, Portable Signal Transduction Assays — BacMam-Enabled Cellular Assays
|New Antibodies for Hyperosmotic Stress Research — ABfinity™ Recombinant Rabbit Monoclonal Antibodies
Brighter Apoptosis Detection — New Alexa Fluor® 488–Annexin V Apoptosis Detection Kits
Protein Sample Prep Gets a Premiere Upgrade — Click-iT® Protein Enrichment Kit
- Buzzworthy — Improved Diagnosis of Venom Hypersensitivity
- The View — Live-Cell Imaging of Neurons Using CellLight® MAP4-RFP
- In the Field — Join the Attune™ Cytometer Revolution
- On the Web — New Resource for Cell Cycle Analysis by Flow Cytometry
- What's New — Subscribe to ADME/Tox News!
New! Expanded Content Now Online
AVAILABLE NOVEMBER 2010 — Bring the Power of Fluorescence Quantitation to Your Bench — The Qubit® 2.0 Fluorometer
The Qubit® 2.0 Fluorometer is the next generation in DNA, RNA, and protein quantitation. Central to the Qubit® Fluorometric Quantitation Platform, this instrument was designed with one thing in mind: to enable you to easily and accurately quantitate your nucleic acid or protein sample, to help avoid wasted samples or time repeating experiments.
what it offers
- Accurate, specific, and sensitive—fluorescence-based dyes bind only to DNA, RNA, or protein
- Easy to use—improved, intuitive touch-screen interface
- Save your data—data logging function and USB port
how it works
The Qubit® 2.0 Fluorometer is an easy-to-use analytical instrument featuring advanced optics, data analysis algorithms, and an intuitive touch-screen user interface. The instrument is designed to work seamlessly with Qubit™ assays for DNA, RNA, and protein quantitation. The integrated design of the instrument and the assays makes the Qubit® 2.0 Fluorometer far more sensitive and accurate than UV absorbance; this helps to avoid the repetition of experiments due to inaccurate nucleic acid or protein measurements.
- Notify me when the Qubit® 2.0 Fluorometer is available
The PrestoBlue™ reagent is a resazurin-based solution that functions as a cell viability indicator. The reagent contains a cell-permeant compound that is blue and virtually nonfluorescent. When added to cells, the reagent is modified by the reducing environment of the viable cell, turns red, and becomes highly fluorescent.
what it offers
- Save time—incubation step as short as 10 minutes
- High-quality results—large assay window and z' values
- Experience convenience—homogeneous add-and-read format
The PrestoBlue™ reagent is a simple add-and-read cell viability assay. Once a plate containing cells and compounds is prepared, the PrestoBlue™ reagent is added, and results can be read in as little as 10 minutes. PrestoBlue™ reagent allows for reliable, high-quality cell viability data and continuous live-cell monitoring and assessment after measuring viability.
- Learn More about PrestoBlue™ Cell Viability Reagent
Reliable, high-quality cell viability data.
Jurkat cells were plated in cell culture medium in a 384-well plate in quadruplicate starting at 100,000 cells/well with a 2-fold serial dilution. Fluorescence measurements were taken after 10 min of incubation and again after 16 hr of incubation with PrestoBlue™ reagent.
| Product
| Quantity
|Cat. No.
|PrestoBlue™ Cell Viability Reagent
|25 mL
|PrestoBlue™ Cell Viability Reagent
|100 mL
BacMam-enabled Cellular Assays are an efficient, easy-to-optimize, and robust method for interrogating specific signal transduction events in a cell background of choice. These assays combine BacMam-mediated gene delivery and LanthaScreen® TR-FRET technology to provide unique benefits including portability into different cell types and a quick, HTS-amenable assay readout.
what they offer
- Portable—compatible with most cell types, including primary and stem cells
- Fast—develop assays typically in less than 1 week, without having to generate stable cell lines
- Flexible—measure multiple modifications such as phosphorylation, acetylation, or ubiquitination
Compared to other types of cellular assays, BacMam-enabled Cellular Assays reduce assay development time by minimizing the need to generate a stable cell line and allowing a choice of cellular background. Once cells are transduced with the BacMam reagent, LanthaScreen® technology is utilized for the readout. This simple, "addition-only" protocol requires minimal hands-on time.
- Learn More about BacMam-enabled Cellular Assays
BacMam-enabled Cellular Assay workflow.
Cells are treated with BacMam reagent encoding a GFP-fusion protein and plated in 384-well format. 24-hours posttransduction, the cells are stimulated to induce the posttranslational modification of the GFP-substrate (e.g., phosphorylation, as shown). Cells are then lysed in the presence of a terbium anti–modification-specific antibody prior to the LanthaScreen® assay readout.
| Product
| Quantity
|Cat. No.
|BacMam Histone H3 [AcLys9] Cellular Assay Kit
|1 kit
|BacMam Histone H3 [pSer10] Cellular Assay Kit
|1 kit
|BacMam AKT [pSer473] Cellular Assay Kit
|1 kit
|BacMam AKT [pThr308] Cellular Assay Kit
|1 kit
|BacMam p53 [AcLys382] Cellular Assay Kit
|1 kit
|BacMam p53 [pSer15] Cellular Assay Kit
|1 kit
New Antibodies for Hyperosmotic Stress Research — ABfinity™ Recombinant Rabbit Monoclonal Antibodies
what it is
FAK phosphorylation at tyrosine 861 regulates hyperosmotic stress signaling and has been indicated as a requirement for mammalian cells to survive under hyperosmotic stress. EGFR is also activated in downstream hyperosmotic stress signaling cascades. Invitrogen now offers two new ABfinity™ rabbit recombinant monoclonal antibodies to help elucidate phosphor-FAK Y861 and EGFR signaling.
what it offers
- Consistency—recombinant antibodies enable consistent results
how it works
ABfinity™ recombinant rabbit monoclonal antibodies help ensure consistent antibody performance lot after lot, so you don't have to revalidate dilutions for your experiments when you order more. FAK [pY861] ABfinity™ Recombinant Rabbit Monoclonal Antibody is phosphospecific to tyrosine 861 and will not cross-react with the non-phosphorylated Y861 form of FAK. The EGFR and FAK [pY816] ABfinity™ antibodies are validated in western blotting and immunofluorescence applications.
- Learn More about ABfinity™ Recombinant Rabbit Monoclonal Antibodies
Immunocytochemistry of HeLa cells labeled with rabbit anti-EGFR.
HeLa cells were labeled with rabbit anti-EGFR (10 µg/mL). Alexa Fluor® 488 goat anti–rabbit IgG was used at 1:1,000 as the secondary antibody. Shown is a composite image of DAPI (blue), anti-EGFR (green), and Alexa Fluor® 594 Phalloidin (red).
| Product
| Quantity
|Cat. No.
|FAK [pY861] ABfinity™ Recombinant Rabbit Monoclonal Antibody
|100 µg
|EGFR ABfinity™ Recombinant Rabbit Monoclonal Antibody
|100 µg
The new Alexa Fluor® 498–Annexin V Apoptosis Detection Kits detect apoptotic cells using recombinant annexin V conjugated to the Alexa Fluor® 488 dye, and dead cells using propidium iodide (PI). After treatment with both probes, apoptotic cells fluoresce green, dead cells fluoresce red and green, and live cells show little or no fluorescence.
what they offer
- Better data—the use of Alexa Fluor® 488 dye produces brighter, more photostable Annexin V conjugates
- Simple conversion—Alexa Fluor® 488 dye easily fits into the same filter configuration as FITC
- Convenience—ready-to-use assays with validated flow cytometry protocols
- More economical—reduced price per test for larger and smaller kit formats
In apoptotic cells, phosphatidyl serine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thus becoming exposed to the extracellular environment. Annexin V labeled with a fluorophore or biotin can be used to identify apoptotic cells by binding to extracellular PS. The Alexa Fluor® 488 dye has proven to make brighter and more photostable bioconjugates than fluorescein (FITC), providing better separation and population resolution. Propidium iodide stains necrotic cells with red fluorescence.
- Learn More about Annexin V Conjugates for Flow Cytometry
Apoptosis detection by flow cytometry.
Camptothecin-induced Jurkat cells were stained with the recommended concentration of annexin V conjugate for 30 min, then washed, resuspended, and counterstained with propidium iodide. Cells were analyzed on the Attune™ Acoustic Focusing Cytometer using a 488 nm laser source and 530/30 nm and 575/24 nm emission filters.
| Product
| Quantity
|Cat. No.
|Dead Cell Apoptosis Kit with Annexin V Alexa Fluor® 488 and PI, 50 assays for flow cytometry
|1 kit
|Dead Cell Apoptosis Kit with Annexin V Alexa Fluor® 488 and PI, 250 assays for flow cytometry
|1 kit
|Annexin Binding Buffer, 5X concentrate for flow cytometry
|1 kit
The Click-iT® Protein Enrichment Kit allows for the efficient capture of click chemistry–based azide-modified proteins on a resin of alkyne agarose. Bound protein is then released by standard proteolytic release upstream of protein sequencing. Superior to biotin- or lectin-based approaches, the kit is ideal for proteomics, LC/MS/MS-based biomarker discovery, PTM analysis, and more.
what it offers
- Allows for global differential profiling of newly expressed (nascent) and posttranslationally modified (PTM) proteins
- Improves signal to noise by eliminating nonspecific interactions and increasing selectivity
- Allows for characterization of protein and PTM stability and turnover rates
The azide click chemistry modification can occur via metabolic feeding, enzymatic addition, or chemical reaction. Click-azide modified proteins or their posttranslationally modified forms are enriched from protein extracts on the alkyne-agarose resin supplied with the kit. Once anchored to the resin via copper-catalyzed click chemistry, extensive washing yields a highly enriched population of nascent molecules that can be further characterized by mass spectrometry. The alkyne-agarose resin improves upon existing biotin approaches by >8 fold, with a signal-to-noise ratio of 25 for the Click-iT® resin vs. 3 for biotin.
- Get Details on the Click-iT® Protein Enrichment Kit
Comparison of Click-iT® protein enrichment technology and biotin-streptavidin protocols.
| Product
| Quantity
|Cat. No.
|Click-iT® Protein Enrichment Kit, for click chemistry capture of azide-modified proteins
|1 kit
Recombinant phospholipase A1 (Ves v 1) from yellow jacket venom for improved diagnosis of hymenoptera venom hypersensitivity
Seismann H, Blank S, Cifuentes L et al. (2010) Clin Mol Allergy 8:7.
In certain individuals, the sting of a yellow jacket can induce life-threatening anaphylaxis. Efforts to develop reliable tests to identify sensitive individuals and produce efficient treatments for patients with yellow jacket stings have been hampered by the scarcity of purified marker allergens. In yellow jacket venom (YJV), phospholipase A1 (Ves v 1) and antigen 5 (Ves v 5) are primarily responsible for IgE-mediated allergic reactions, and these nonglycosylated proteins are unique candidates for diagnosis of YJV allergy. Although expression of Ves v 5 had previously been demonstrated, Ves v 1 could only be produced in an insoluble form. In a recent publication by Seismann and colleagues, the researchers were able to demonstrate the secretion of recombinant Ves v 1 and Ves v 5 in insect cells in soluble form. Using a colorimetric phosphlipase test (EnzChek® Phospholipase A1 Assay Kit, Cat. No. E10219/E10221) they showed that rVes v 1 retained its enzymatic activity, and both proteins were able to stimulate basophils isolated from venom-allergic patients. The authors concluded that purified recombinant allergens Ves v 1 and Ves v 5 hold promise as valuable tools in the search for yellow jacket venom allergy diagnosis and treatment.
| Product
|Cat. No.
|EnzChek® Phospholipase A1 Assay kit for 2 plates
|1 kit
|EnzChek® Phospholipase A1 Assay kit for 10 plates
|1 kit
|Join the Attune™ Cytometer Revolution
The positive reviews are rolling in from researchers who've experienced the Attune™ Acoustic Focusing Cytometer.
"Overall, I would say that the Attune seems to be not only revolutionary, but…also a significant step in the improvement of the existing technology."—Tomas Adejumo, UCL Scientific Support Services
During a recent installation, a researcher was so anxious to get started on the new technology he remarked to the installing engineer: "You make it work, I will make it famous!" Other customers are excited about the blue/violet laser configuration, which enables them to monitor cell health, proliferation, apoptosis, and cell cycle, along with their fluorescent proteins excited by the blue laser. Stay tuned for publications and more comments from thrilled Attune™ Cytometer customers.
|Live-cell imaging of neurons using CellLight® MAP4-RFP. Hippocampal tissue from postnatal day 3 rats was harvested and dissociated in neural culture medium, then plated onto glial feeder cultures. Cells were resuspended at 50,000 cells/mL in complete neural culture medium plus mitotic inhibitors. The medium was removed from the glial feeder cultures and replaced with 2 mL of the neural cell suspension. CellLight® MAP4-RFP (50 μL) was added to the plates, and cells were incubated overnight. Fluorescence was observed in neurons after 48 hr in culture.
|New Resource for Cell Cycle Analysis by Flow Cytometry
Flow cytometry provides a powerful tool to assess cells in G0/G1 phase versus the S phase, the G2 phase, or polyploidy. Now we've made it easier to select the right cell cycle assay for your flow cytometry experiments:
|Live-cell imaging of neurons using CellLight® MAP4-RFP. Hippocampal tissue from postnatal day 3 rats was harvested and dissociated in neural culture medium, then plated onto glial feeder cultures. Cells were resuspended at 50,000 cells/mL in complete neural culture medium plus mitotic inhibitors. The medium was removed from the glial feeder cultures and replaced with 2 mL of the neural cell suspension. CellLight® MAP4-RFP (50 μL) was added to the plates, and cells were incubated overnight. Fluorescence was observed in neurons after 48 hr in culture.
|New Resource for Cell Cycle Analysis by Flow Cytometry
Flow cytometry provides a powerful tool to assess cells in G0/G1 phase versus the S phase, the G2 phase, or polyploidy. Now we've made it easier to select the right cell cycle assay for your flow cytometry experiments:
Molecular Probes® The Handbook
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