text:
- Read the latest issue of the ProbesOnline E-Newsletter.
ProbesOnline Special Edition: Another chance to see the most popular products and technologies featured in ProbesOnline during 2009.
The Next Generation of Antibodies — ABfinity™ Recombinant Monoclonal Antibodies
Still Using BrdU? — New and Improved Click-iT® EdU Cell Proliferation Assays
Illuminate Internalization Pathways in Real Time — pHrodo™ Dextran for Endocytosis
Cell Proliferation and Cytotoxicity Assays — CyQUANT® Direct Cell Proliferation Assay
Live Cell Cycle Analysis With Limited Cytotoxicity — Vybrant® DyeCycle™ Ruby Stain
NEW Cell & Tissue Analysis Products
See all of this month's New Products for Cell & Tissue Analysis
Visualize Tight Junctions and the Cytoskeleton — Alexa Fluor® 488 Dye–Conjugated Primary Antibodies
Live-Cell Imaging and Fixed-Cell Analyses — Organelle Lights™ Reagents
Live-Cell Imaging of Cell Cycle and Division — Premo™ FUCCI Cell Cycle Sensor
Take Charge of Your Cell Counting — Count Blood Cells With the Countess™ Automated Cell Counter
Optimize Cell Viability Assays for Multicolor Panels — LIVE/DEAD® Fixable Dead Cell Stain Sampler Kit
Check out the latest issue of BioProbes
Nanotechnology Meets Cellular Analysis
Subscribe to BioProbes
ABfinity™ antibodies are the next generation of antibodies, presented exclusively by Invitrogen. These antibodies are generated by cloning the specific antibody genes and producing them in a mammalian expression system. ABfinity™ technology provides the most specific antibodies available, bringing you highly reproducible results.
what they offer
- Unmatched lot-to-lot consistency
- High sensitivity and specificity
- Extensive validation and characterization
Generating antibodies by expressing cloned antibody genes ensures that every lot of ABfinity™ antibodies produces the same results. These antibodies offer maximal sensitivity and specificity, allowing you to detect small amounts of only your intended targets. ABfinity™ antibodies are also extensively validated and characterized, giving you the confidence you need, right out of the tube—no further optimization is required.
- Learn More about ABfinity™ Recombinant Monoclonal Antibody Technology
- Search for Primary Antibodies
HeLa cells labeled with ABfinity™ rabbit monoclonal antibody against the eukaryotic initiation factor 4EBP1 [pT37] in the absence of peptides (left), and in the presence of a phosphopeptide (center) or nonphosphopeptide (right) used as an immunogen. Detection was performed with Alexa Fluor® 488 goat anti–rabbit IgG. Actin was stained with Alexa Fluor® 568 phalloidin.
|Reactivity: validated (expected)
|Cat. No.
|PKC-θ [pT538]
|Hu (X, Rt, Ms, Cp, B)
|100 μg
|Hu (X, Rt, P, Ms, Eq, Ch, B)
|100 μg
|Mnk1 [pT197/pT202]
|Hu (Z, X, Sw, Rt, P, Ms, Mk, Eq, Cp, Ch, Cn, B)
|100 μg
|Reactivity: B, bovine; Ch, chicken; Cn, canine; Cp, chimpanzee; Eq, equine; Hu, human; Mk, monkey (rhesus); Ms, mouse; P, primate; Rt, rat; Sw, swine; X, Xenopus; Z, zebrafish
Applications: F, flow cytometry; ICC, immunocytochemistry; IF, immunofluorescence; IHC, immunohistochemistry; IP, immunoprecipitation; WB, western blotting
The Click-iT® EdU assay is a superior alternative to traditional methods for detecting and quantitating newly synthesized DNA. The Click-iT® EdU imaging and HCS kits now include a 1X ready-to-go reaction buffer—eliminating stock solution preparations and careful additions of reagents. Also included is a new Click-iT® reaction rinse buffer that eliminates any foaming caused by the previous BSA-based wash buffer for crisp imagery and better data.
what it offers
- Rapid detection—results in only 30 minutes
- No DNA digestion or denaturation—preserve cell morphology, antigens, and dsDNA integrity
- Streamlined protocol—fewer steps to results
EdU is a nucleoside analog of thymidine, and is incorporated into DNA during active DNA synthesis. Detection is based on a copper-catalyzed covalent "click" reaction between the alkyne present on EdU and the azide present on the Alexa Fluor® dye. The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions, in contrast to BrdU assays that require DNA denaturation (typically using HCl, heat, or digestion with DNase).
- Learn More about Click-iT® EdU Cell Proliferation Assays
Effect of Click-iT® reaction rinse buffer on HCS CellMask™ blue staining in U2OS cells. (A) No rinse buffer was used. (B) Click-iT® reaction rinse buffer was used.
|Cat. No.
|Click-iT® EdU Alexa Fluor® 647 Imaging Kit *for 50 coverslips*
|1 kit
|Click-iT® EdU Alexa Fluor® 488 HCS Assay *2-plate size*
|1 kit
|Click-iT® EdU Alexa Fluor® 488 HCS Assay *10-plate size*
|1 kit
pHrodo™ dextran is a superior alternative to other fluorescent dextran conjugates (e.g., BCECF and tetramethylrhodamine (TRITC)) for live-cell imaging of endocytosis.
what it offers
- Content-rich results—red-orange fluorescence facilitates multiplexing with blue, green, and far-red fluorescent fluorophores
- Simple method—minimal fluorescent signal at neutral pH eliminates quenching reagents and extra wash
pHrodo™ dextran has a pH-sensitive fluorescence emission (excitation and emission maxima of 560/585 nm) that increases in intensity with increasing acidity. This increase is particularly dramatic in the pH range of 4 to 8, commonly seen as endocytic vesicles are acidified. pHrodo™ dextran is essentially dark in the extracellular environment. Upon internalization, the acidic environment of the endosome elicits a bright, red-fluorescent signal that can be visualized by fluorescence microscopy, flow cytometry, or high-content imaging and analysis.
- Learn More about pHrodo™ Indicators
Multiplexed analysis with pHrodo™ dextran for endocytosis. HeLa cells were transduced with Organelle Lights™ Endosome-GFP. The following day, medium was replaced with serum-free medium plus 50 µM deferoxamine. Cells were washed and incubated with 1 µg/mL Hoechst 33342, 10 µg/mL pHrodo™ Dextran, and Alexa Fluor® 647 transferrin for 5 min at 37°C. (A) Organelle Lights™ Endosome-GFP; (B) Alexa Fluor® 647 transferrin; (C) pHrodo™ dextran.
The CyQUANT® Direct assay is a fluorescence-based cell proliferation and cytotoxicity assay that is well suited for high-throughput screening applications.
what it offers
- Convenience—no washes, cell lysis, or temperature equilibrations required
- Accuracy—cytotoxicity data compare well to metabolism-based assays
- Robust results—readout is independent of metabolic state
The CyQUANT® Direct assay is based on a cell-permeant DNA-binding dye in combination with a masking dye. The masking dye blocks staining of dead cells and cells with compromised cell membranes so that only viable cells fluoresce. Because DNA content is highly regulated, cell number estimates using the CyQUANT® Direct assay are very accurate.
- Learn More about the CyQUANT® Direct Cell Proliferation Assay
CyQUANT® Direct assay protocol. The CyQUANT® Direct assay is designed for use with multi-well plates (96-, 384-, or 1,536-well formats), making it ideal for high-throughput screening applications. The reagent is added directly to cells in complete medium and incubated for 30 to 60 min. Samples are read from the bottom on standard fluorescence plate readers set for green fluorescence (e.g., Alexa Fluor® 488, fluorescein, fluo-4, and GFP).
|Cat. No.
|CyQUANT® Direct Cell Proliferation Assay
|1 kit
|CyQUANT® Direct Cell Proliferation Assay
|1 kit
Vybrant® DyeCycle™ Ruby Stain is a near-infrared emission stain for DNA content analysis, enabling live cell cycle analysis with limited cytotoxicity.
what it offers
- Live cell cycle analysis
- Limited cell toxicity offers cell sorting potential
- Compatible with any flow cytometer
The Vybrant® DyeCycle™ Ruby Stain penetrates the cell membrane of intact cells and binds covalently to the DNA in the nucleus. Staining is simple—suspended cells are incubated with Vybrant® DyeCycle™ Ruby Stain and fluorescence is measured directly. No additional treatment or centrifugation is required. This dye takes advantage of the commonly available 488 nm and 633/635 nm excitation sources with emission >670 nm, leaving common blue laser channels open for other studies. And unlike DRAQ5, Vybrant® DyeCycle™ Ruby Stain does not kill the analyzed cells, offering the possibility of cell sorting based on DNA content.
|Cat. No.
|Vybrant® DyeCycle™ Ruby Stain
|400 assays
|Vybrant® DyeCycle™ Ruby Stain
|100 assays
Invitrogen offers one of the most extensive portfolios of antibodies for cell junctions and the cytoskeleton. We have released new Alexa Fluor® 488 conjugates for key cell junction and cytoskeletal targets.
what they offer
- Save time and costs by eliminating secondary antibody detection
- Ideal for imaging multiple targets in colocalization studies
- Validated against multiple applications
Alexa Fluor® 488 (excitation/emission 494/517 nm) is one of the brightest dyes available for imaging applications. Its conjugation to highly specific antibodies results in bright, crisp images with almost no background, and bypasses the need for secondary antibody detection. The new Alexa Fluor® conjugates of antibodies against occludin, claudin-1, claudin-4, claudin-5, and α–tubulin are valuable tools for imaging tight junctions and the cytoskeleton, allowing easy colocalization with other key markers.
- Learn More about the Alexa Fluor® 488 Dye
Human Caco-2 cells stained with mouse anti-α–tubulin Alexa Fluor® 488, showing different phases of mitotic cell division. DNA is counterstained with blue Hoechst 33258.
|Species reactivity
|Cat. No.
|Mouse anti-claudin-1,
Alexa Fluor® 488 conjugate
|Cn, Hu, Ms, Rt
|100 µg
|Mouse anti-claudin-4,
Alexa Fluor® 488 conjugate
|Cn, Hu, Ms, Rt
|100 µg
|Mouse anti-claudin-5,
Alexa Fluor® 488 conjugate
|Hu, Ms, Rt
|100 µg
|Reactivity: Cn, canine; Hu, human; Ms, mouse; Rt, rat
Applications: ICC, immunocytochemistry; IF, immunofluorescence; IP, immunoprecipitation; WB, western blot.
Molecular Probes® Organelle Lights™ reagents are prepackaged and ready-to-use fluorescent protein constructs fused with signal peptides for accurate and specific targeting to subcellular compartments and structures. The collection of Organelle Lights™ reagents has recently expanded to include new colors for staining lysosomes, mitochondria, endoplasmic reticulum (ER), and endosomes.
what they offer
- Ready-to-use reagents—no potentially harmful cell treatments required
- Simple method—optimize transduction conditions only once per cell type
- Versatility—multiplex with other stains in live or fixed cells
Organelle Lights™ reagents consist of suspensions of baculovirus carrying an expression construct with a fluorescent protein fused to either a cellular protein or a localization peptide. Simply add the virus suspension to your cells, incubate overnight, and you're ready for imaging. Organelle Lights™ reagents can label a broad range of mammalian cell types, including primary and stem cells, without the need for lipids or dye-loading protocols that can perturb cell growth and viability.
- Learn More about Organelle Lights™ Reagents
|Live-cell imaging with Organelle Lights™ ER-RFP. HeLa cells were transduced with Organelle Lights™ ER-RFP. The next day, cells were co-stained with 50 nM MitoTracker® Deep Red 633 and 1 µg/mL Hoechst 33342 (stains nuclei). Imaging was performed on live cells using a DeltaVision® Core microscope and standard DAPI/TRITC/Cy®5 dye filter sets.
|Cat. No.
|Organelle Lights™ Lysosomes-GFP
|1 kit
|Organelle Lights™ Mito-RFP
|1 kit
|Organelle Lights™ ER-RFP
|1 kit
The Premo™ FUCCI cell cycle sensor enables live-cell imaging of cell cycle and division. As cells progress through the cell cycle, nuclear fluorescence changes from red to green.
what it offers
- Ready-to-use—no need to purify plasmids or worry about vector integrity
- Cell-friendly—no harmful treatments are required; primary and stem cells are labeled without apparent cytopathic effects
- Titratable—defined reagent concentrations enable precise titration and optimization of expression levels
The Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) is a fluorescent protein–based sensor that employs red (RFP) and green (GFP) fluorescent proteins fused to different regulators of the cell cycle: Cdt1 and geminin. The temporal regulation of these proteins by ubiquitin results in the biphasic cycling through the cell cycle. Premo™ FUCCI Cell Cycle Sensor takes this technology one step further by using the BacMam gene delivery system—the prepackaged genetically encoded reagents are ready for immediate use. Simply add the Premo™ FUCCI reagents to your cells, treat with the BacMam enhancer, wash, incubate overnight for protein expression, and visualize cell cycle progression in populations of cells using fluorescence microscopy.
- Learn More about Premo™ FUCCI Cell Cycle Sensor
Imaging cell cycle progression in live cells with Premo™ FUCCI Cell Cycle Sensor. (A) Schematic of cell cycle progression with nuclear fluorescence changes. (B) U2OS cells were transduced with Premo™ FUCCI Cell Cycle Sensor, then stained with Alexa Fluor® 647 wheat germ agglutinin.
The Countess™ Automated Cell Counter uses trypan blue staining combined with a sophisticated image analysis algorithm to provide accurate cell and viability counts in just 30 seconds. The instrument also measures the average size of live, dead, and total cells to give you all the data you need from your cell cultures without using a hemocytometer.
what it offers
- Accurate—eliminate the subjectivity and variability of manual cell counting
- Fast—count live and dead cells,and measure viability and average cell size in 30 seconds with just 10 μL of sample
- Convenient—no setup, cleaning, or service required
The Countess™ Automated Cell Counter can accurately count blood cells including peripheral blood mononuclear cells (PBMCs), red blood cells (RBCs), and white blood cells (WBCs) from lysed whole blood and Ficoll preparations. Because the Countess™ instrument uses trypan blue staining, the viability of WBCs is easily determined, while platelets and noncellular debris are unstained and excluded from the count. The Countess™ instrument does not differentiate between WBC types, and viability information is not available for RBCs.
- Learn More about Countess™ Automated Cell Counter
- Download the Countess™ Blood Cell Protocol
|Cat. No.
|Countess™ Automated Cell Counter
Countess™ Automated Cell Counter Starter Kit
|1 kit
|Countess™ Cell Counting Chamber Slides
|50 slides (100 counts)
Optimize Cell Viability Assays for Multicolor Panels — LIVE/DEAD® Fixable Dead Cell Stain Sampler Kit
what it is
LIVE/DEAD® cell viability assays are used to differentially stain live and dead cells in a variety of mammalian cell types. These cells can then be fixed with formaldehyde for subsequent analysis by flow cytometry. The LIVE/DEAD® Fixable Dead Cell Stain Sampler Kit contains all 8 LIVE/DEAD® reactive dyes that are excited with laser lines ranging from UV to far red, making it easier to identify the optimal dead cell stain for multicolor panels.
what it offers
- Post-fixation analysis—staining pattern preserved
- Accuracy—eliminates staining artifacts
- Flexibility—broad dye selection
how it works
LIVE/DEAD® cell viability assays employ the reaction of a fluorescent reactive dye with amines on cellular proteins. Viable cells exhibit faint staining on the cell surface, whereas the dye penetrates the interior of dead cells, resulting in at least a 50-fold increase in fluorescence compared to live cells. Because the dye reacts covalently with proteins, the staining pattern is completely preserved following sample fixation with formaldehyde.
- Learn More about Fixable Viability Dyes for Flow Cytometry
Live-cell gating using LIVE/DEAD® Fixable Violet dye eliminates staining artifacts from analysis. In a comparison between live-cell gating using scatter (A), and live-cell gating using LIVE/DEAD® Fixable Violet dye (B), staining artifacts using scatter gating are illustrated. The significant number of dead cells in subsequent analysis using scatter (C) are noted, as compared to the use of the LIVE/DEAD® Fixable Violet dye (D) to eliminate dead cells. Reproduced with permission from Elsevier ((J Immunol Methods 313:199 (2006)).
Molecular Probes® The Handbook
©2009 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Feel free to distribute ProbesOnline to friends and colleagues, but please keep this copyright statement intact.


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The Road to Becoming A Social Business #SMWSF #SMWCisco
If you have browsed the Social Media Week agenda, your eyes have probably caught a panel discussion on The Road to Becoming A Social Business. We're thrilled to have Michael Brito (@britopian), SVP Edelman Digital and author of the book, Smart Business, Social Business moderate this panel with Adobe, SAP, Wells Fargo and Intel. We're going to cover questions such as: How do you hold people accountable? How do you organize for social? How do you create or find content that make great stories? How do you encourage content innovation and experimentation while protecting your brand? How do you scale? And many more questions along the same lines. In addition to the discussion, our panelists will leave you with some practical tips you can take back with you right away. If you plan to attend this session live or on the Cisco UStream channel, be ready to ask your questions in person or via social media using the #SMWCisco hash tag. (Note: the hash tag for the entire week is #SMWSF but we have set up a hash tag specific to our day at Cisco to help better navigate through the questions related to our event.)
Drum roll please… immediately following the panel, Michael will be signing his book, Smart Business, Social Business. The book walks you through the internal transformation of organizations into a social business and describes the key factors to be considered in this evolution. To learn more, check out our video interview with Michael. (By the way, we had a great time shooting the video interview. Check out a video montage of bloopers below.)
Intrigued? Order a copy on Amazon today and bring it with you on February 14. This book makes a great Valentine's Day gift so while we're at it, grab a book for your friend, sweetheart or colleague.
We hope to see you at our event and at the book signing. Please pass on this message! Thanks in advance.
Video Interview with Michael Brito, Bloopers:
In an effort to keep conversations fresh, Cisco Blogs closes comments after 60 days. Please visit the Cisco Blogs hub page for the latest content.
1 Comments
I love the outtakes. That was a hilarious addition to the post…

subset: commoncrawl


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Warranty Information (EU)
We're disappointed if you're not completely satisfied with your GoPro purchase. We take a lot pride in our products and service! If you are experiencing a problem with a GoPro purchase, please take a minute to read the information below to determine if your issue is covered under the GoPro warranty.
MONEY BACK GUARANTEE: (GoPro Webstore Purchases)
We want you to be totally psyched about the products you purchase from GoPro's webstore at gopro.com. If at any time within 30 days of receiving your order, you are not 100% satisfied, you may return it to us for a refund*. No questions asked. Any items damaged due to reasons not covered under warranty cannot be accepted back for refund under our Money Back Guarantee. Also, associated shipping fees are not refundable. NOTE: this guarantee only applies to orders placed on GoPro's webstore at gopro.com.
*Money Back Guarantee excludes the following products: Karma with Hero5 Black, Karma for Hero5 Black, Flight Kit for Karma Grip, Karma Core, Karma Stabilizer, Karma Grip, and Karma Controller (collectively "Karma Products"). You may return undamaged Karma Products purchased from GoPro's webstore within 14 days of receipt if you are not 100% satisfied. To qualify for a refund, return your Karma product in the ORIGINAL box, including device, charger, battery, instructions and other components. All merchandise must be in like new condition. Shipping charges may apply.
If you bought your product directly from GoPro.com, and you are not satisfied with your purchase within the conditions described in the above Money Back Guarantee, you can return it following the below procedure. Your card will be credited upon receipt of the returned merchandise.
- Request an RMA (Return Merchandise Authorization) number from GoPro Customer Support by clicking on the Contact Us link at the bottom left of the main Support Page.
- Package products along with a copy of the RMA form.
- Write the RMA number on the outside of the box and send to the appropriate address based on your specific location: (items must be received by GoPro within 14 days of issuing the RMA):
Note: Please do not hand deliver your product to the RMA facility, even if you are local. These locations are not setup to receive visitors. Thanks!
|GoPro Returns
14530 Innovation Drive
Riverside, CA 92518
|GoPro Returns
Smakterweg 100
5804 AM Venray
The Netherlands
|GoPro Returns
51 Ubi Avenue 3
Singapore 408858
GoPro will replace your product or part free of charge if your product or part has a manufacturing defect that is covered by the warranty below.
- BEFORE sending anything to GoPro, please contact our Customer Support Team by clicking on the Contact Us link at the bottom right of the main Support Page. We are often able to solve the problem quickly via email.
- If our Customer Support Team determines that your problem qualifies under warranty coverage, you will be issued an RMA (Return Merchandise Authorization) number
- Prepare a package to return to GoPro.
- Please include your original receipt and a clearly written explanation of the problem
- Write your RMA number on the outside of the box and send to the appropriate address based on your specific location:
Note: Please do not hand deliver your product to the RMA facility, even if you are local. These locations are not setup to receive visitors. Thanks!
|GoPro Warranty Service
14530 Innovation Drive
Riverside, CA 92518
|GoPro Warranty Service
Smakterweg 100
5804 AM Venray
The Netherlands
|GoPro Warranty Service
51 Ubi Avenue 3
Singapore 408858
Returns that don't qualify for warranty coverage will be returned to customers. It is vital that you communicate with GoPro's Customer Support Team before sending in your return. Please contact us by clicking on the Contact Us link at the bottom right of the main Support Page.
The statutory – legal guarantee covers the productions defects of the products and of the accessories GoPro for a period of 2 years from the original purchase day and this guarantee can be enforced by the consumer within 2 months from the discovery of the defect. We suggest, for this reason, to keep your receipt (tax receipt at purchase) or other similar document received when you pick up the product. In case of conformity defect, the consumer is entitled, at its own discretion, to the repair or the substitution of the defected products by the seller, without any additional cost, save if the requested remedy is not possible or too much expensive in respect of the other. If repair or substitution are not possible, the consumer has, in any case, the right to have a reduction of the price or to have a sum proportioned to the product value paid back, against the return of the product to the seller. The above applies ONLY to consumers as defined by the Law.
Damages deriving from the use or from an accident or caused by the natural wear and tear do not fall within this guarantee or other guarantees. GoPro denies any responsibility deriving from incidental or consequential damages caused by the misuse of the products or its components. The guarantee does not cover the loss of the box and the damages which can derive from such loss and this is because of potential errors made by the users in closing. Please read and follow carefully the instructions for the hermetic closure of the boxes.
Questions? Comments? Experience some world class customer support when you contact our Support Team by clicking on the Contact Us link at the bottom right on the main Support page. We'd love to hear from you!


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County buys SUV for EMS chief
Marion County Commission voted Monday to purchase a new sport utility vehicle and medical equipment for the county's ambulance department.
A Ford Expedition will be purchased from the federal General Services Administration for $25,874, about $6,000 less than it would cost from local dealers, Emergency Medical Service director Steve Smith said.
A Dodge Durango, it replaces Smith's vehicle that will be passed down to Durham first responders, and the Durham first responders' van will be sold online.
The commission also approved purchase of two automatic ventilators and two intravenous pumps for $7,600 total. They will be kept at St. Luke Hospital and Hillsboro Community Hospital for when ambulances make emergency transfers from the hospitals.
The automatic ventilators will free emergency medical technicians to provide other care. The intravenous pumps will allow the administration of up to three medicines or fluids.
EMS crews reported 74 calls, including 18 cases in which transport to a hospital was refused, last month. Total ambulance calls this year are virtually unchanged from 2012.
The Marion ambulance was the busiest, with 29 calls. Hillsboro responded to 20, Peabody, 17; Florence, four; Tampa, three; and the Marion back-up unit, one. Goessel and Lincolnville first response units each went to three calls, and Ramona first response went to one. Durham, which will receive the Durango, had no calls.
An EMT-Basic class has been completed, and 11 of the 12 students have passed their practical skills exams. Four of the students have passed their written exam, and several more will take the written exam June 18.


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Shut up, already, damn
Billy five boroughs
Henry David Thoreau
Something like a street chic Philippe Candeloro
They can't hold a candle to my triple Salchow
Wow, Dow, Jonestown
Massacre, Ow, Blaow, Blaow, Pow, Pow
Like, what we gon' do now?
More than two clowns, two brown man
Likkle youth like the fountain
Where the sound man?
Get down, clan
Sit down, man
Sit, sit, sit down, man
Truth be told, you rappers shoulda quit it
Me, I'm gettin' head, yeah I'm something like a fitted
New Era, all written, smitten
Kittens purr for us, 'cause we bitchin'
Can't stand the heat?
Then get the fuck out the kitchen
Shut up, shut up, dude
Shut up, shut up, dude
Yo on a pie like Reddi Wip
Just watched The Last Dragon, like Freddie did
The Nidal Malik Hasan of this rap shit
Guns like the body of human truth of moving proof
Cliff Notes for uncouth schooly unruly youth
Google the groovy truth and go back to your hootie-hoo
Tootsie rolls, stanky legs, booty-dos
Gringita no lo entienda, but her coochie do
Coochie-coo, smoocheroo
Twenty-three skidoo, where is the didgeridoo?
Aboriginal flavor packet in pocket of the raider jacket, soviet issue
Potato basket, sadomasochistic, lipstick thespian
Jada Pinkett Smith versus Wesleyan
Wealthy friends, failed Marxists, stale heartless
Hailed artists, frail, sensitive, pale sales targets
Shut up, shut up, dude
Shut up, shut up, dude
Body language like you nervous
Me, I'm burnin' Ls son, Pervis
Fat man make joke like Gervais
Ricky, but more than joke, purpose
Itchy and Scratchy Obatala fish chakra
Sriracha hot pasta sauce, pop rock
Bottle pop, Dayquil, Nyquil, cable, Bible, Tae Bo
Paco Ignacio nacho supreme
Bald like eagles
Style like free throws
Equal, ???? beaucoup for new you
Rap's audible, laudable, more than plausible, your raps is pauseable
Reverse King Midas, capital monetizes me
Anthropology couldn't analyze my pottery
All the bloggers be like "This I gotta see
One of the late aughts' great race oddities"
Shut up, shut up, dude
Shut up, shut up, dude
Das Racist keeps on rapping on this closing track, despite being kindly asked to "Shut up, dude".


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UPPER MERION - A few days shy of his 281st birthday, Gen. George Washington unsheathed his ceremonial sword and cut the birthday cake made for him by his wife, Martha, in front of hundreds of cheering onlookers Monday at Valley Forge National Historical Park.
'It is indeed moving beyond words. I cannot express how grateful I am to see such a wonderful group of people here today,' the future president told the crowd. 'I just want to thank you all so much for being here today.'
The event, part of the park's Presidents Day weekend of events that included the 101st Annual Boy Scouts of America Pilgrimage and Encampment and historical re-enactments at the park's Muhlenberg Brigade area, also marked the 235th anniversary of what is believed to be the first public celebration of Washington's birthday, which Martha Washington staged while her husband and his troops were camped out at Valley Forge.
Along with opportunities to dress in Colonial garb to take photos with General Washington and several of his officers, children in attendance were able to 'enlist' in the Continental Army, where they could learn marching and maneuvering techniques based on the training concocted by Gen. Friedrich Wilhelm von Steuben and utilized at Valley Forge. The youngsters could also do various crafts, including writing birthday cards for Gen. Washington, making tri-cornered hats out of construction paper, and weaving on a cardboard loom.
The birthday celebration, which has become an annual event for the park, was organized by park staff in conjunction with the Encampment Store, the Friends of Valley Forge and the Valley Forge Convention and Visitors Bureau, according to Rhonda Schier, the chief of interpretation and education for the park.
'(The groups) all work together for several months to set up the activities, invite the guest presenters, get the refreshments ready, which is the birthday cake, and get historical re-enactors in position to lead the kids' muster. It's a real team effort to celebrate Washington's birthday,' Schier said.
According to Schier, this year's crowd was larger than usual, which she credited to the generally pleasant weather Monday afternoon, which enabled visitors to explore other areas in the park in addition to attending the festivities.
'It seems to grow in popularity every year,' she said. 'We are attracting a lot of our neighbors this year. The beautiful weather always helps to bring the visitors and the neighbors to this event. They get to spend some time outdoors in a national park.'
In addition to celebrating the birthday of the park's most famous alumnus, the festivities also helped make the public aware of the park and events that are held there.
'This is a way that we're sort of honoring the birthday party that Martha held for George when turned 46 in February of 1778, and so it's a very significant commemoration of the birthday celebration that was held here then,' Schier said. 'It brings visitors a really fun opportunity to experience their neighborhood national park.'
Follow Tony Fioriglio on Twitter @TheTonyFiorigli.
Information from the Times Herald, www.timesherald.com


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Question: What are the key parts of the eye that help it function properly?
Answer: The eye is like a camera : The front of the eye has a clear window -- the cornea -- that's similar to the cover of a watch. Beneath that we have the pupil, which is surrounded by the iris, which is the color part of the eyes, [it] opens and closes to allow the light to adjust. Beneath that is the lens which focuses the light into an image onto the surface of the retina. The retina is like the film in a camera. The image is then transferred from the retina to the optic nerve.


text:
Education aspires to improve outcomes.
Edmodo creates opportunities to invest in yours.
Edmodo Insights integrates brand new Cambridge University Press content for the reformed linear GCSE qualifications. Using Snapshot, a formative assessment tool with a high quality bank of questions and quizzes aligned to the new curriculum for KS4 Maths and English Language, Insights helps teachers create mini-assessments and delivers valuable progression data in an instant.
To further develop its next-generation learning environment, Edmodo partnered with Microsoft to introduce users to the benefits of cloud productivity technologies. With access to Office 365's award-winning OneNote, PowerPoint, Excel, and Word, teachers and students have the ability to create, edit, and view files within the platform, making it easier than ever to store and share all of their work, all in one place. Sway and Office Mix will also be integrated, along with OneDrive, giving users the option to log into Edmodo using their Microsoft accounts.
Put the "fun" in functional
When you develop web and mobile apps for Edmodo, you reach millions of teachers and make a direct impact in classrooms around the world. Whether your app is free or premium, or tailored to biology, language arts, math, or science, our API makes it simple to seamlessly integrate with our platform's core functionality and its most popular features.
Partnering with Edmodo also means you can test your app from the teacher and student perspective, and gain insight into how it's being used—feedback that allows you to tailor your app even further and help teachers and students make the grade.
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Edmodo Spotlight is the world's largest content-sharing community that empowers educators to discover and collect valuable educational resources. They can also publish their own content, rate and review what's being shared, and voice which resources are most effective.
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The Cybertory Project is a collection of on-line educational simulations supporting a wide range of problem-based exercises in molecular biology, molecular genetics, and bioinformatics.
Simulation-based exercises are similar in many respects to laboratory experiments. For example, they generally require running positive and negative controls to tell the difference between a failed experiment and a negative result. Our simulators produce results in the same formats one might find in a lab. This allows exercises to include realistic data analysis procedures, where students must deal with experimental uncertainty. Please take a moment to browse through our collection of exercises in the sidebar at the left to get an idea of the breadth of technologies and applications covered.
Welcome to our worldwide community of users. We appreciate any comments, questions and suggestions you may have. Your feedback supports our efforts to make it easier and more economical to offer sophisticated online problem-based instruction in molecular biology.


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Message Board
Antonia Pantoja Message Board
Talk about the novels, new and used books that Pantoja has written!
Author Pantoja's Book Reviews
Memoir of a Visionary: Antonia Pantoja
This is the struggle of a child from a poor Puerto Rican family in the Island to educate herself, develop her sense of purpose and public responsibility , leadership skills and ethics to become one of the most outstanding Puerto Rican leaders. IT is the struggle of her political consciousness and leadership to become the creator of many major Puerto Rican institutions to improve the conditions of others and to advance the civil rights of Puerto Ricans in...


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SANAA (Reuters) - A Yemeni air force plane crashed in the capital Sanaa on Tuesday, killing at least 12 people, security sources said.
State news agency Saba said three women and two children were among those killed when the plane, on a training flight, came down in a western residential district. Eleven people were wounded, security sources said.
Pictures of the crash on social media sites showed one body near burning wreckage of the aircraft. Several cars were on fire and debris littered the street.
A security official said the pilot had ejected from the plane. There was no immediate word on whether he had survived.
"It's terrible and painful," resident Abdullah al-Ashwal said. "The police and medics evacuated five completely burned bodies, they were all unrecognisable."
Abdulsattar Mohammed said he saw a plane burning near houses that were also set on fire. "People were terrified and ambulances arrived late," he said.
A military official said the plane was a Russian SU-22 fighter/ground attack aircraft.
Yemen has 30 SU-22s and four SU-22UM3s in an air force with 79 capable aircraft in all, according to the 2012 Military Balance handbook issued by the International Institute of Strategic Studies.
In November 2012, a Yemeni military transport plane crashed near Sanaa airport and burst into flames, killing all 10 people aboard.
Reporting by Mohammed Ghobari; Writing by Mahmoud Habboush and William Maclean; Editing by Alistair Lyon


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When I have a sketch loaded in the UNO, the bootloader still appears to run when I powerup the UNO using the battery/external power connector (no USB).I get the three flashes and then the sketch starts after about another half-second.Is that supposed to happen? Or better yet, how is that supposed to happen?
Does it behave the same if you power it up by connecting via USB?(In theory, the bootloader only runs when the reset status register identifies the reset cause as "external reset." "Power-on reset" is supposed to be a separate cause, which should not cause the bootloader to run.)
Not that it's a big deal. Just trying to understand what I have. And I don't understand it yet. Unless, there is some sort of UNO board circuitry that asserts an external reset on power-up.Does anyone else see this behavior with a new UNO (R3)?TheGipp
I suspect the DTR autoreset mechanism is causing your bootloader to run on power up. At least I'm fairly confident this is true when a FT232RL chip is present for USB serial. But I'm only speculating that the 16U2 on the Uno behaves similarly.I demonstrated this to myself by putting an Uno optiboot-flashed 328P on a breadboard and observed that the chip skips the bootloader (no 3 flashes) when power is applied. The 3 flashes show up when the RESET line is taken low, however. The same chip, when inserted into the socket on an Arduino clone went through the bootloader code each time power was applied from either the DC jack or USB.My conclusion is that the power up sequence causes a DTR reset. Don't know if it is the result of activity by the FT232RL, or if the RC network on the reset line caused it.Jim
Well the Uno in rev2 and the present rev3 did add a 1K ohm pull-down resistor on the DTR output pin from the 16U2 chip that the older arduino 2009 board did not use on the FTDI DTR output. I wonder if that pull-down resistor keeps the 328p reset pin low long enough after initial power-up to cause a true pin1 external reset condition and thus activation the bootloader wait period to be active?Lefty


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Veterans around the country are receiving letters from the federal government informing them that because of alleged physical or mental disability their financial decisions will be made by a government-appointed "fiduciary" and they will be forbidden from "purchasing, possessing, receiving or transporting a firearm or ammunition."
This is not a warning of something that might happen; these letters are being sent out everyday by the Department of Veterans Affairs (VA) as part of its Fiduciary Program.
Citing the Brady Handgun Violence Prevention Act, VA is warning veterans labeled as incompetent that they will be "fined and/or imprisoned" if they are found in possession of a gun or ammunition.
The final decision on whether the veteran should be disarmed and prevented from ever owning a gun or ammunition will include an assessment of the following:
• The willingness to serve and abide by all agreements
• An interview with a VA representative
• Credit report review
• Interviews with character witnesses
For years now, the Obama administration has followed a policy of targeting vets, taking away their guns, imprisoning them, and labeling them as "right wing extremists" and potential threats to national security.
Consider this quote from a Department of Homeland Security report entitled "Rightwing Extremism: Current Economic and Political Climate Fueling Resurgence in Radicalization and Recruitment":
The possible passage of new restrictions on firearms and the return of military veterans facing significant challenges reintegrating into their communities could lead to the potential emergence of terrorist groups or lone wolf extremists capable of carrying out violent attacks.
Veterans are being asked to serve tour after tour in unconstitutional foreign conflicts with no identified enemy, then returning home and having their most basic right denied by the very government that asked them to sacrifice their lives.
There was a story last year about a police raid on the house of an Army veteran and Purple Heart recipient in Ohio. The county sheriff executed a search warrant and seized the man's guns for the "safety of the defendant and the general public."
Although unnamed, the government ruled the man was mentally incompetent to handle his own affairs or to own a weapon.
Readers also may remember the recent shocking case of Brandon Raub. The New American's Jack Kenny reported on the story last August. Kenny wrote that Raub was:
held involuntarily as a psychiatric patient at the Salem Veterans Affairs hospital in Virginia over anti-government postings on his Facebook page. Raub, a Marine combat veteran who served in both Iraq and Afghanistan, was visited by FBI and Secret Service agents at his North Chesterfield home on August 16, then taken by police under an emergency custody order to John Randolph Medical Center in Hopewell. He was transferred to the veterans hospital on August 20. A medical assessment of his condition at John Randolph described him as paranoid and delusional, the Richmond Times-Dispatch reported. But after an hour-long hearing in Hopewell Circuit Court August 23, Judge W. Allan Sharrett dismissed an involuntary commitment petition as invalid.
"The petition is so devoid of any factual allegations that it could not be reasonably expected to give rise to a case or controversy," said the release order signed by Sharrett, vacating the order of Special Justice Walter Douglas Stokes to detain Raub for 30 days. Stokes, who presides over commitment hearings, also ordered Raub's transfer to the VA hospital in Salem, about 180 miles from his home. Judge Sharrett said he was shocked to find the commitment order contained no grounds for holding Raub.
The irrefutable fact is that, had the Rutherford Institute not stepped in and defended Raub, the young man would likely still be unconscionably held in a government mental hospital at the mercy of Obama administration bureaucrats.
Apart from the government's reprehensible quest to disarm veterans, brand them as mentally ill, and treat them as terrorists, there is the fact that all this is being done in violation of the veterans' right to due process protected by the Fifth Amendment.
The Fifth Amendment to the Constitution provides that no person shall be "deprived of life, liberty, or property, without due process of law."
As in the case of so many other constitutional impediments to its statist goals, the president and his subordinates are ignoring the Fifth Amendment and arresting, disarming, and imprisoning veterans without due process.
Of course, the VA assures veterans subject to disarmament that if they file a timely objection to the decision, a VA board will consider their appeal. This is hardly the rigorous legal process required before the government can constitutionally deprive citizens — or any person — of their most basic constitutional rights.
Why is the president targeting veterans? Michael Connelly proposes the following possible explanation:
The reality is that Obama will not get all of the gun control measures he wants through Congress, and they wouldn't be enough for him anyway. He wants a totally disarmed America so there will be no resistance to his plans to rob us of our nation. That means we have to ask who will be next. If you are receiving a Social Security check will you get one of these letters? Will the government declare that you are incompetent because of your age and therefore banned from firearm ownership. It certainly fits in with the philosophy and plans of the Obama administration. It is also certain that our military veterans don't deserve this and neither do any other Americans.
Or, as Michael Snyder wrote at the American Dream, "The federal government wants lots of warm bodies to throw into battle, but when those bodies get broken the government is not there to pick up the pieces."


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16310 Bratton Ln Ste 200 Austin, TX 78728
Ph: (512) 402-6644 E-Mail: firstname.lastname@example.com
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Our future isn't about Buck Rogers and spaceships that cruise to far-away galaxies at the speed of light.It's about family.As I get older, I frequently think about what the future holds.What will it be like?Marble and bronze statues are erected to famous folks and heroes so they will be remembered by future generations.But I often wonder if those who stand on their shoulders will even care.Ten months ago, I was blessed with a great-grandson who will carry my family name into the future.Hopefully he will make a difference and bring credit to the name entrusted to his care in a po
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Your House Here
Editorial Rating:
Average Rating: ( 0 votes)
Your House HerePosted by Jeffrey Rous at March 04. 2013
Ok, so we designed and built our house. Sketchup became my favorite tool and late at night while taking a break from looking for cheap modern faucets, learning about tankless waterheaters, etc., I doodled.
I have an entire folder of houses that I couldn't get out of my head until I tried to put them on paper. I think I may put a few of them up here. I hope others feel free to do the same.
This one was actually plan B for the lot we built on. Because of the roof pitch regulations, the first version had a gable. Then I flatened it out.
I have long been intrigued with the idea of second floor living. And here was one of my attempts at that.
A neighbor is thinking about buying several lots that span from one street to another. So the lots would be 30'x120' with streets at both ends. He asked what I would do with that I gave him a few options. The courtyard is 21'x12' and faces north. The long roof extending from the living room is to shade the afternoon sun (it gets hot in Texas).
Re: Your House HerePosted by Jeffrey Rous at March 06. 2013
I have been fascinated by Corbusier's Villa Savoye. Here is a modern update with a smaller footprint (I have another version with a small patio on the second floor).
So some friends were building a house and said that they wanted a Frank Lloyd Wright type house. After looking thorough some of my books, it turned out they just wanted a simple house with a hipped roof. I threw a couple ideas together, but their builder's architect had what they really wanted, a standard suburban floor plan. In any case, here is what I came up with.
Here is the other... with a nod towards not only FLW Winslow house, but also Ralph Rapson's Dwell House entry. I prefer the master up and family room down, but with some "windows" to below, it would be cool.
Two years back some friends were adding a pool. They wanted a cabana that could serve as guest house and workout room (incl. treadmill).
I did this, and even worked up a set of plans (anyone want a copy?). They liked the blue, I liked the white. In the end, I instead did a steel and Polygal wing for over the patio. I'll post that sometime soon.
Glenn Murcutt has this great house with a courtyard that includes a pool. Who needs a yard right? Could that work on a more suburban lot? I like that it has a lot of little places between places.
And here is my attempt at a FLW MCM fusion. I like courtyards. Two bedrooms, a study and a screen porch for dining.
Re: Your House HerePosted by Gladys christabel at March 15. 2013
Very Neat Picture Presentation
Re: Your House HerePosted by Jeffrey Rous at March 17. 2013
Thanks! I'd like others to jump in with their amateur designs. I was just thinking I should "modernize" a few of the "soft contemporary" designs. I fear a "pitched roofs! WTF?" response. Easy to flatten a roof an "mod-up" the windows.
I was looking at one of the above houses, and as much as I like how the hipped roofs intersect perfectly, I decided to modernize it a bit. I love front porches and screen porches... so I added one.
It sort of interests me how building codes can alter not just a singular design, but an entire stylistic wave. Take the egress window requirement. Essentially, at least one window in every bedroom must be 30" wide by at least 42" tall. The Eames house with its horizontal awnings wouldn't pass code. This makes a horizontal theme for the fenestration difficult unless you use a door instead of a window in each bedroom. So this either leads to a horizontal theme or you get crazy and mix up squares and rectangles to get it more horizontal (a chaotic or random theme). In our case, we used a lot of squares and two of the bedrooms have 60"x60" window units divided vertically to get a 30"x60" egress window without going too vertical.
Re: Your House HerePosted by Jeffrey Rous at March 23. 2013
I almost forgot this one. I actually drew up plans. Two bedrooms on the ground floor and two small offices on the main living floor. I tried to do the framing so I wouldn't need the posts, but it was too much of a pain. I like the second floor patio.
Re: Your House HerePosted by Jeffrey Rous at April 04. 2013
Honestly, I am not sure where this one came from. I just like the idea of a outdoor patio on the side of a house, but on the second floor. Two exteriors, one more modern, one sort of post modern, but would fit (perhaps) in a more McMansion-y neighborhood.
Re: Your House HerePosted by Cathy Dawkins at May 19. 2013
congrats on the new house! looks great!


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Computer Science ColloquiumIm Rahmen des Informatik-Kolloquiums, das von den Instituten des Fachbereichs Informatik, der Österreichischen Gesellschaft für Informatik (ÖGI), der Arbeitsgemeinschaft für Datenverarbeitung (ADV) sowie der Österreichischen Computergesellschaft (OCG) abgehalten wird, spricht
Prof. Yoshihide Horiuchi, Ph. D., email@example.com
University of Shizuoka, Japanüber das Thema:
Transcultural Communications in IFSR/ISI Conversations, Systems Design and Business PracticeZeit: Mon 26.4.2004, 15:30, 60 Minuten
Ort: TNF 711
ZusammenfassungThe presentation begins with description of transcultural communications at the IFSR Fuschl and ISI Asilomar Conversations. Next, explanation of the planned Japan Conversation follows: Its aim, designed process and expected outcomes. The Japan Conversation uses English and Japanese as the official languages, and will be based on the Japanese culture. It was developed at the 1994 and 1995 Asilomar Conversations. Then, findings of the Year 3000 Idealization Team at the 2000 and 2002 Fuschl Conversations will be discussed Next will be a discussion with the audience on comparing business practice in Austria and Japan: Appreciation of cultural differences and incorporating them into transcultural communications in business. Another matter of discussion is the cultural differences in systems design between Austria and Japan. In Japan, systems design means more of systems engineering, rather than idealization-based social systems design. Japanese culture is strong in tacit knowing but weak in explicit knowledge. In other words, they are good at apprenticeship and on-the-job training, but not so good at developing work-procedure manuals. The presentation concludes with an attempt to list potential transcultural research topics.
Vortragender1973 B.A. in Law, Waseda University, Japan, 1973 Marketing Executive, Foreign Trade Department, Taisho Pharmaceutical Company Limited, Tokyo, Japan, 1976 M.S. in Advertising, University of Illinois at Urbana-Champaign, USA, 1980-84 Instructor, MBA Program and Marketing Department, LaSalle University, Philadelphia, USA, 1984 Ph.D. in Social Systems Sciences, The Wharton School, University of Pennsylvania, USA, 1984-88 Director, Socioatomic Public Relations Company, Tokyo, Japan, 1988-Present Associate Professor/Professor, School of Administration and Informatics, University of Shizuoka, Japan,
Einladender: o. Univ.-Prof. Dr. Gerhard Chroust
Liste aller Vorträge


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Its so tight on that side of the engine, it's going to be so difficult to get to the timing chain fully. Cam covers off will be the first thing to do, which means removing the inlet manifold, labour intensive just that. The oil will need dropping and the timing chain cover taking off, this will mean removing quite a number of items including the crankshaft pulley, auxiliary belt and tensioner.
It may be quicker just to pull the engine, for much easier access though. Hoists can be rented from tool shops.
Good luck!
. Any advice on getting to the timing chain with the engine in situ please? Working on my driveway and it's small and I don't have a hoist or room to use one really so leaving the engine in the car would be a lot easier for me if abit fiddly.
You're going to have change quite a few items over to make it work in an X-Type. The water pump for a start is on the opposite side on an X-Type so it can fit in the tight engine bay, different auxiliary belt. You're going to have to do some work closing off the hole on the rear of the head as that's where the water pump runs off.
There's quite a few differences, best bet is to put them both together and work out what needs swapping over. It is the same block and basically same heads but with quite a few small differences.
If it were me, I'd have dropped in a 3.0L.
Hello again I have just looked at the block number and it is as follows RF-XW4E-6015-AS if that helps on the S-Type
Hello Ian my name is Paul, I have been told your a genius when it comes to Jaguars, I was after abit of info please. I have bought a replacement engine for my 2002 51 plate X-Type 2.5V6 sport, only the engine that has turned up is a 2001 S-Type 2.5V6 engine. Is there a way to use this engine? I noticed it doesn't have VCT on the top valve covers, would I have to change the heads? Is the block even the same? Thankyou for your time reading this.
You need the antenna pack from Jaguar, it's £155 and has all the stuff you need to connect the speed pack spoiler aerial to your car and bypassing the old pop up one.
Hi Ian,
I'm new to the forum and was looking at a post you started regarding the ariel upgrade. I have recently purchased a 2006 model and have just bought a speed pack spoiler with the built in ariel and wondered what other items I would need to complete the job? Totally agree the cars look so much better without the mast ariel. Any pointers you could give me would be greatly appreciated.
Senior Member


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Pocket as many pool balls as you can before the time runs out!
Survive the cold, the hunger, and the radiation in this post-apocalyptic world. Collect resources, build a shelter and improve your equipment. Explore the abandoned houses and the irradiated city. Fight the ghouls and other players!Help
There are no awards for this game!


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Finally, the night has come. Adrienne and Brandi are going to be at the same place, at the same time.
Will it get violent? How much of her own foot can Brandi fit in her mouth? Will they finally give the rest of the Housewives something new to talk about?
First things first -- the Lisa/Kyle showdown. Lisa is finally fed up with Kyle riding so hard for Adrienne, without any measure of consideration for anyone else.
They sit down for drinks and Lisa (who seems way more upset by this than you'd think she'd be) wants to hear Kyle say, with her own mouth, that she has her back. Kyle doesn't want to fight over something so petty, and Lisa, bless her heart, doesn't blow up at this.
How is it "petty" to want your friend to support you from time to time? To be held accountable for stoking arguments and then backing away? To give you the benefit of the doubt? Get it together, Kyle. This is not cute.
Lisa knows their friendship will never be the same, but she isn't ready to dead it yet.
After some back and forth and a little melodrama, the women call a truce. Wonder how long that'll last?
Brandi's hanging with Yolanda now, and I am all for it. Yolanda's chilled out a little on trying to prove to us that she's perfect (read: coming for Martha Stewart's wig) and has proven to be one of the more level-headed cast members.
In fact, and I can't believe I'm saying this, Yolanda is a perfect example of the kind of "Real World" cast member I thought I would be when I was a teenager. The voice of reason, who is ready to read all of the silly housemates at any time. With vigor.
Anyway, Brandi and Yolanda take Yolanda's daughter, Bella, to her horseback riding lesson. While Bella's off being adorable (Yolanda really does have gorgeous kids) Brandi opens up about being worried about seeing Adrienne for the first time at Lisa's tea party.
Mostly, Brandi's afraid she will actually combust if she has to keep her mouth shut and her butt out of court. And this is why it would make so much sense to NOT INVITE HER AND ADRIENNE TO THE SAME FUNCTIONS!
The last person I expected to throw one of these misguided group events was Lisa. And a tea party to welcome Adrienne back into the group, no less. You know this is going to end in tears.
Anyway, Yolanda tells Brandi that she has done the right thing by admitting that she was wrong. She needs to continue to move on. Adrienne is now the one in the wrong, milking the drama until the end.
But without it, what would Adrienne's storyline be this season? Get it how you live.
It's time for tea! Brandi shows up first, with a gift for the hostess: a vibrator. The fact that I instantly knew it was a sex toy of some kind is a problem.
Marisa shows up next, still complaining about her husband like this isn't going to be broadcast on national television -- and then spread all over the internet. Lisa, who has clearly been hanging out with Brandi too long, suggests that she just have a threesome, or "bring in some outside talent." Girl.
Anyway, Yolanda's pep talk barely worked. Brandi's still freaking out over Adrienne's simple behind, and to make matters worse, Kyle shows up with Faye, who clearly wasn't invited because Lisa can't stand her, either.
Kyle is just so bold with her troublemaking these days. Who can blame Kim for staying home and out of the bull.
Let us address this frigging decadent tea party Lisa just threw for these ungrateful women. Mainly, I'm obsessed with the centerpieces -- they can barely see each other over the tops of those gorgeous things. I've been stanning so hard for Lisa this season, I hope she Googles herself, discovers these recaps and invites me to the next one.
Everything's relatively ok -- until Tipsy Taylor swaggers over to the table and confronts Adrienne about having her lawyer send Brandi The Letter. So now Adrienne wants us all to believe that Brandi just made all of this up out of thin air -- there is no lawsuit. Forget about the fact that she's been throwing around the words "law suit" like nothing for the past six episodes. Forget about the fact that anyone with access to the Internet knows that she and Paul were threatening to leave the show over Brandis remarks.
And ... we're right back where we started. That Kyle/Lisa feud had promise -- why can't we just go with that?
Finally, the night has come. Adrienne and Brandi are going to be at the same place, at the same time.


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By Joyce P. Brayboy, U.S. Army Research LaboratoryJune 26, 2014
ADELPHI, Md. (June 26, 2014) -- Champ was the majestic canine in the picture sitting, his dark ears erect, and eyes focused on his master, awaiting the next command.
The vivid image that Stephen Lee presented of the dog that saved his friend's life was the driving force behind his exploring enhancements for Military Working Dogs supporting Soldiers in harm's way.
In Valeri Lantz-Gefroh's improvisation class, she taught techniques that Lee, who is the chief scientist at the Army Research Office, could use to talk more effectively about science. Lantz-Gefroh held the half-day session as part of the Alan Alda Center for Communicating Science workshop at U.S. Army Research Laboratory in Adelphi, Md.
"The goal of teaching scientists improv is to free them to talk about their work, to pay attention to their listeners and to connect with their audience," said Lantz-Gefroh.
"We feel honored as listeners when someone shares a big moment with us," she said. Lantz-Gefroh's 25 years as a theater professional have taught people how to connect with experiences through the feeling they experience with others. Her trainees are often overwhelmed by the thought of connecting with an entire room of people. But, "finding connection with a couple of people in the room can bring the entire room together," she said.
The interpersonal connection is critical as ARL moves toward bringing person-to-person collaborations between Army researchers and partners from academia, other government agencies, industry and nontraditional innovators to the laboratory in a shared physical space.
"We have to communicate the value of our basic and applied research to our partners and other audiences," said Thomas A. Moyer, ARL's public affairs director. "ARL's scientists and engineers have the ability to find the far-reaching technologies that define the Army of the future, and also ensure our continued military superiority. We have a very important story to tell."
"Trust between Soldiers, leaders and the American people is key," Moyer said.
About 20 scientists explained what they call "far-reaching" ideas to three critics at the workshop. This first exercise started a series that brought experts from the safety and comfort of their seats behind long tables, to the center of the floor to "mirror," "pitch" and "zap" into a new way of thinking about communication. Communicating science with clarity can help scientists make their work better understood by the audiences they serve, according to the Alda Center website.
"The interactive session was about keeping the participants available, aware and connected, and remembering that communication is a two-way street," said Jenna Brady, ARL public affairs specialist, who coordinated the workshop visit.
"The laboratory had never had a workshop like this before, so the participants did not know what to expect," Brady said. "Instead of the familiar lecture, the group experienced games like Zip-Zap-Zop."
"It was a comfortable atmosphere. When the scientists did not feel the need to be so technical in speaking, it helped us understand underlying meaning behind their research. After a few exercises, participants let their guards down and spoke candidly about the science behind our troops."
"Competence, character and commitment shine through when our workforce feels free to be authentic," Moyer said. On June 14, the nation's leaders established the Continental Army, which commemorates the total force -- Active, Guard, Reserve, Civilians and Families -- and their continued commitment to serve this nation.
Celebrating the Army's 239th birthday this month will be "our opportunity to reflect on the past professionals who have shaped Defense science and technology, and to become more comfortable with telling future stories about science in straight-forward language," Moyer said.
Like Lee's Military Working Dog picture, the goal of a good science story is to begin with the person behind the research.
Lee recently mentored students, who developed a new bite sleeve that mimics the tactile sensation and puncture resistance of skin for Military Working Dog bite training. The Bite Sleeve team won Best Overall Project at Senior Design Day up against 84 other engineering teams that vied in the North Carolina State University competition.
When Lee simulated petting a dog as a gesture of great significance for him, it was more than an improvisation exercise.
It was another step he took toward helping him help people understand why the Army would study Military Working Dogs.
The U.S. Army Research Laboratory public affairs office will host communicating science workshops quarterly. ARL welcomes your feedback about communicating Army science, email firstname.lastname@example.com.
About Army Research Laboratory
The U.S. Army Research Laboratory, a part of the U.S. Army Research, Development and Engineering Command, has the mission of providing innovative science, technology and analyses to enable full-spectrum operations. RDECOM is a major subordinate command of the U.S. Army Materiel Command. AMC is the Army's premier provider of materiel readiness -- technology, acquisition support, materiel development, logistics power projection, and sustainment-- to the total force, across the spectrum of joint military operations. If a Soldier shoots it, drives it, flies it, wears it, eats it or communicates with it, AMC provides it.


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Permanent Midnight is a weekly Complex Pop Culture column where senior staff writer, and resident genre fiction fanatic, Matt Barone will put the spotlight on the best new indie horror/sci-fi/weirdo cinema, twisted novels, and other below-the-radar oddities.
You have to admire Israeli filmmaker Ari Folman's gutsiness. Does that mean you also have to give him a pass for how often impenetrable The Congress—his two parts live-action and one-third animated follow-up to the critically beloved, and fully animated, 2008 film Waltz with Bashir—is, particularly in its latter half? No, but the experience of entering his conceptually heightened version of Hollywood is, despite its flaws, exhilarating.
Of the same mind as Leos Carax's 2012 meta-cinematic oddity Holy Motors, The Congress thrives on the audacity of cynicism, clamping on to Hollywood's most destructive practices and going all wack-a-doo with what can best be described as psychedelic satire. It's easy to see why Folman's futuristic cautionary tale won the top Fantastic Features prize at last year's Fantastic Fest—it's a cinephile-specific product, and anyone whose film adoration is borderline or less should steer clear, lest they want to simulate taking a hallucinogenic drug without any of the fun.
Narcotics play a large role in The Congress, Folman's loose, less anti-communist and more screw-you-Hollywood adaption of author Stanislaw Lem's 1971 trippy sci-fi novel The Futurological Congress. Playing a fictionalized version of herself, but representing every middle-aged and older actress in the business, Robin Wright headlines as a movie star at the end of the line. Her loving but no-bullshit agent (Harvey Keitel) tells her like it is—she's past her prime, and if she doesn't start making expensive, popcorn-friendly genre films instead of respectable dramas, it's over for her.
The head of Miramount Studios (a stand-in for Paramount, natch), a suitably insensitive soul-crusher (Danny Huston), takes it one step further: Wright's only hope of maintaining her Hollywood stock is to participate in a radical new process known as "sampling," which allows the real-life actor to fade away into anonymity and age while their "scanned" versions go on starring in whatever movies the studio wishes while remaining young and beautiful. Against her better judgment, Wright signs up, mainly due to the fact that her teenage son, Aaron (Kodi Smit-McPhee) is sickly and needs her undivided, stay-at-home attention. Through scanning, she'll be able to care for him while digitized equivalents of herself cash out in ridiculous blockbusters like RRR: Rebelo Robot Robin - Street Fighter.
The opening, reality-centered section of The Congress is scathingly raw in its views on Hollywood's bottom-line ageism. Kudos to Wright for enduring the film's meta shots fired her way, the most biting of which come from Huston's character: "I need Buttercup from Princess Bride. I need Jenny from Forrest Gump. I don't need you. I need you only for your history… In the economy of a scanned actress, you're not worth six bucks." One can imagine actresses like Susan Sarandon, Patricia Clarkson, and Diane Lane watching The Congress and sobbing, if not shivering at its dark yet entirely feasible depiction of the film system's treatment of the movie game's veterans.
When The Congress transitions into its dystopian animation section, though, those same actresses may feel more confused than compassionate. Abandoning the more simplified cutout style he used in Waltz with Bashir, Folman illustrates the world where a "Restricted Animated Zone" includes a high-rise hotel full of disillusioned industry types who drown their sorrows in hallucinogen chemicals. It's an alternately beautiful and dangerous hybrid of Ralph Bakshi drawings and Disney playfulness—a punk rock Disney, if you will. Though it's visually striking, the film's post-scanning storyline is tough to crack. Miramount has traded in moviemaking for pharmaceuticals, with Wright designated as the company's spokesperson to unveil a new product, since she's universally recognized as the "Best-Ever Digital Actress." Before Folman fleshes out this more Stenislaw Lem-minded portion, though, assassins and terrorists bring forth a violent, chaotic uprising that's rushed, not revolutionary.
For Wright, The Congress is laden with irony, whether intentional or not. Dominated by the film's animation, which doesn't allow her to capitalize on her heartbreaking performance within the pre-scanning scenes, she's ultimately the byproduct of exactly what The Congress is attacking: great actors and actresses being hindered by CGI and digital advancements. She does seem to be in on the joke, though, indicated by a funny back-and-forth between her and Huston about Wright's reluctance to make a science fiction movie while, you know, The Congress technically is one. Folman spends so much time developing his far-reaching, muddled faux Hollywood, however, that Wright's nuanced performance is a casualty of his ambition.
Then again, there's no mystery as to what compelled Wright—a multiple award nominee who's currently a regular on House of Cards—to make The Congress her first sci-fi project. Despite his inability to make all of the film's moving parts connect, Folman has brought so much earnestness and heart to The Congress, specifically in its handling of the mother/son dynamic between Wright and Smit-McPhee, that its inaccessibility isn't a deal-breaking deterrent. At times frustrating, yes, but still awe-inspiring enough to qualify The Congress as a worthwhile entry into the "Hollywood is a heartless, unappreciative place" canon of films, preceded by Sunset Boulevard (1950), Barton Fink (1991), and the aforementioned Holy Motors.
The Congress is available on VOD now, via Drafthouse Films, and will open theatrically on August 29.
RELATED: Permanent Midnight: The Year's Best Indie Genre Movies (So Far)
RELATED: Permanent Midnight: The Year's Best Genre Fiction Books (So Far)


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Jessica Alba and Her Family at Disneyland
Jessica Alba Takes a Magical Family Trip to Disneyland
Jessica Alba and Cash Warren took their daughters to Disneyland for two days of fun on Sunday and Monday. The family of four visited the main park and California Adventure, where they checked out multiple rides and games. Jessica and Cash rode Space Mountain and the group also hopped on Pirates of the Caribbean and Indiana Jones, and posed with a holiday-themed Mickey Mouse. Their trip to the Happiest Place on Earth came during a break from Cash's work on his latest producing project, In the Blood, which he's been filming with Cam Gigandet since last week.
The Alba-Warrens are staying busy as the year comes to a close, but they still have time to make a mark in our Best of 2012 coverage. Make sure to vote if they happen to be your favorite celebrity family, or if you think Jessica had the best bikini moment of the last 12 months.


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Nice knowing you all
Gardening tips and questions.
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Nice knowing you all
The garden is infested with spiders the size of a 50p, they are everywhere so if I don't post again I've been eaten alive. I think I may wake up cocooned in a web tomorrow. Not normally freaked by spiders but these have freaked me. I think they may be european garden spiders...big buggers. Funnily enough I spent all last saturday morning at the local walk in centre with a swollen hand having been bitten whilst gardening.....wonder if it was one of these I didn't see.
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Thrifters unite! Using basic sewing skills and an eagle eye for the good stuff at yard sales, you'll turn cast-off clothes into cute accessories.
Project Upcycle shows you thrifting and garment construction techniques that you'll use again and again. You need basic sewing skills -- and an eagle eye for the good stuff at rummage sales -- to make projects like cute cashmere shrugs from old sweaters and flirtatious wrap skirts from vintage tea towels. Celebrate your exit from the corporate world and liberate your suit jackets and dress shirts into saucy berets. With Betz's upcycling magic, your shirt cuffs become billfolds to hold the cash you save, and you'll learn endless variations on felted totes, limited only by your imagination and your stash of old wool sweaters. Watch each lesson in Project Upcycle on your own schedule, at your own pace, as many times as you like. If you enjoy creating unique looks that are simple, sustainable and fun, sign up for Project Upcycle: Thrifty Sewing Projects.
If you're not delighted with your Bluprint experience, we'll make it right or give you a full refund.


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Wednesday, April 21, 2010
The Underdog
While writing my new novel after FLESH, I came across Gap Creek (Robert Morgan, Algonquin Books) and read it with much admiration. Published by a small press, it became the Oprah Book Club Selection in 2000, perhaps boosting the initial press run from 5000 to 500000 copies. Then last Monday, news came about the 2010 Pulitzer Prize fiction winner: Tinkers (Paul Harding, Bellevue Literary Press). Both Algonquin Books and Bellevue Literary Press are small. The BLP is so small The New York Times said, "Up in a tiny office on the sixth floor of Bellevue Hospital Center sits the most unlikely tenant in this 271-year-old public institution: a fledgling publishing imprint. . . ."
Well, what most realistic chance does a small press have against the New York publishing behemoths when it tries to vie for the nation's top literary prizes? Read this: "A book by an unknown author, from a new and nearly unknown press, lands on a reviewer's desk. What are the chances it will command her attention?" —Hartford Courant, January 4, 2009
Both novels above were published by small literary presses. I thought about this fact. Then I recalled the 1999 Kiriyama Prize awarded to a fellow Vietnamese writer I know, Andrew Pham, for his first work of non-fiction Catfish and Mandala. I always have this thought: Can the judges be unbiased when they read the work by a first-time author, published perhaps by a small literary press, alongside those works by veteran authors, e.g., Philip Roth, Ha Jin, from the submissions for the award? Well, in 1999, Andrew Pham beat out the other finalist, John Dower, who won The Pulitzer Prize, The National Book Award and The Bancroft Prize for his work Embracing Defeat: Japan in the Wake of World War II, which was on the Kiriyama's short list that year.
Is it true that small literary presses publish more superior works than the mainstream publishers? Well, many small presses are labors of love, according to Charlie Hughes, publisher of Wind Publications. Therefore, they are extremely selective in what they publish. As such, "chances of acceptance are statistically low. Most small presses have more books waiting to be published than their time or financial resources will allow." This does not surprise me. My novel, FLESH, was accepted by the award-winning Black Heron Press in August 2009. Yet it's slated for the publisher's 2011 publishing list.
Considering the doldrums of our publishing business, one has to wonder why readership has been declining. Is the quality of books being fed to readers the main culprit?
Yet there is fairness in the process of selecting a winning title for a literary prize.
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REAL ESTATE- $old
Margaret B. Ober to Lawrence Barnett III, 2.048 acres at 6818 Jarman's Gap Road, Crozet, $375,300.
Jonie R. Muncy, trustee, to Household Realty Corp. of Virginia, 3526 President's Road, $128,647.
Alexander E. Salomon to Commonwealth of Virginia, parcel, $62,785.
Federal Home Loan Mortgage Corp. to Daniel G. Brookhart, 2962 Magnolia Bend, $303,200.
Highlands West LP to Craig Enterprises Inc., parcel in Western Ridge, $120,000.
Professional Foreclosure Corp. to Federal National Mortgage Assoc., parcel in Avon Park, $264,500.
Tribal Properties LLC to NVR Inc., parcel in Abington Place, $588,000.
Guiseppe Finazzo to Christian J. Mcfarland, 87 Georgetown Green, Georgetown Green, $205,000.
Cricket Hill Homes Inc. to F. Barry Robertson III, 6913 Harvest Farms Lane, Harvest Farms, Crozet, $730,000.
Fannie Mae to Theresa L. Mead, parcel in Esmont, $127,100.
Ted Wilson Glass to Blanka Owensova, 3621 Keswick Road, Royal Acres, Keswick, $272,100.
Glenmore Associates Ltd. to Sansovich Development LLC, 144.779 acres, $2,236,619.
Glenmore Associates Ltd. to Neal M. and Tessa G. Sansovich (Sansovich Development LLC), 141.276 acres, $2,123,406.
Glenmore Associates Ltd. to Russell C. Sykes, 21.103 acres at 3620 Victoria Lane, Keswick, $538,236.
Glenmore Associates Ltd. to John M. H. Hart Jr., 115.895 acres, $1,894,698.
Glenmore Associates Ltd. to Richard E. Austin, 21.199 acres, $558,604.
VanderLinde Housing Inc. to Edward E. Stonesifer, 0.890 acres at Foxchase, $232,600.
Sally E. Henderson to Frederic B. Osgood, parcel in Peacock Hill, $330,000.
Kevin B. Glover to Ahmed Properties LLC, condominium unit at Parkside at Eagle's Landing, $109,400.
Marie V. Spradlin to Heavenly Homes LLC, 1.0 acres on State Route 618, $14,000.
North Pantops Townhouses LLC to NVR Inc., condominium unit at the Pavilions at Pantops, $308,400.
W. Stephen Scott, trustee, to Howard S. Byrd, parcel at Hickory Ridge Farm, $116,000.
Steve Thompson to Commonwealth of Virginia, parcel, $36,780.
Arc of the Piedmont Inc. to Commonwealth of Virginia, parcel, $28,900.
Margaret C. Lehman to Carol B. Koch, parcel, $186,800.
David S. Hawkins to Giuseppe Finazzo, 2968 Magnolia Road, Forest Lakes, $299,000.
Margaret H. Weatherly, trustee, to Karen J. Kehoe, 1411 Courtyard Drive, Redfields, $289,965.
Lynn Lytton Hamer, trustee, to William W. Foshay III, 203 Devon Road, Ednam Forest, $1,076,000.
David C. Leckrone to Gary D. and Laura J. Calese, 1661 Robin Hill Court, Raintree, $345,000.
Stanley O. Ikenberry, trustee, to Lindsay G. Dorrier Jr., 5.236 acres at 278 James River Road, Scottsville, $251,853.
Michele Jordon, trustee, to Barbara D. Newton, 113 Dorset Court, Camelot, $157,000.
Roger D. Hutchins to American International Relocation, 2053 Aviano Way, Fontana, $409,000.
American International Relocaton to James V. Nardone, 2053 Aviano Way, Fontana, $409,000.
Lillian D. Kizer to Courtney R. Roane, 170 Jordan Lane, Clover Lawn Village, $200,000.
Michael A. McGee to Shane M. McGee, 143.51 acres at 1644 Lost Valley Road, Earlysville, $75,000.
Deborah A. Smith to Barbara Thompson, trustee, 3.096 acres at 1309 Mountain View Street, $212,800.
Highlands West LP to Craig Enterprises Inc., parcel in Western Ridge, $130,000.
Eugene H. Scott to Mary Lee MacDonald, 369 Minor Ridge Road, Wynridge, $249,000.
Big deal:
Glenmore Associates Ltd. to Sansovich Development LLC, 144.779 acres, $2,236,619.