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New Treatments for Thinning Hair
From our pals at The New York Times. Another surgical approach to balding. I've been reading about this procedure for a while, though I'm not sure it is for everyone. It's a surgical procedure, which means shelling out money and having down time, but this might be a viable solution for many women.
New Treatments for Thinning Hair
Have you had this procedure or know a woman who has?


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Before you begin promoting, we highly recommend that you play around with it for a few days to ensure everything is working properly. To start, have your employees beta test the app with you by downloading it before the initial launch. In addition, make sure to get all of your employees up-to-date on any special in-app offers. This way, no one is surprised when clients try to use the coupons at your location.
Once you're done, it's time to push the app out to all of your customers.
1. Promote via social media!
Post your app listing URLs on your Facebook, Twitter, Google+, and blog. To find your URLs, follow the instructions in this article. If the app is hosted in our Developer accounts, we can send these URLs to you.
2. Promote using QR codes!
Place QR codes on your website, at your business, on flyers & business cards, in your monthly newsletters and direct mail, and in your email signature. People can use QR scanner apps to scan the QR codes, which lead them straight to the app listing.
Select the "Promote" button at the top right of your app dashboard to download/print your QR codes. Make sure you've first followed these instructions and tested out your QR codes to verify they're directing properly. If they're not working properly, please email us to confirm that your App Store and Play Store URLs have been added properly.
Tips on Searchability
When your app first goes live, you'll probably notice it's not at the top of the list when you search in the App Store/Google Play. This is normal for a new app, but there are some things you can do to gradually increase its visibility.
- Have your app users rate it highly and leave comments about the app. The more feedback, the more visibility.
- A unique app name will narrow search results so users don't have to swipe through pages and pages before they find yours.
- Include specific keywords that are not generic.
- Try to make the app description as unique as possible, including the app name and keywords within it. Repetition is essential.

subset: commoncrawl


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Sunday is Little Miss Heidi's birthday and she'll be THREE years old. I can't believe that it's been 3 years...sigh
Anyway, Aunt B, Hannah, and Eli all came to see Heidi Friday night to celebrate with her a couple days early since they won't be able to see her on Sunday.
We spent a little time at the fall festival, but not before heading up to the studio to finish tearing up the rest of the flooring.
I got it all tore up. The second half was MUCH easier than the first. The seperation wall supports between the reception and shooting areas is now complete. RJ and Heidi's room and the furnace room supports should both be completed tomorrow.
Drywall'ing will begin next week. Here are a few pictures of Aunt B and the kids as well as one more progress picture.


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Read Reviews, Compare Prices & E book
All I know is I do know this place from other bloggers who have been to Morocco. This a part of Morocco was not even part of my plans however it fell into place so completely and I believe it was meant to be. I took a bus from Rabat the capital of Morocco to Chaouen as the locals call it. Mail forwarding services are inexpensive (as little as $10 per month plus postage), protected and handy. If you use them, you mechanically turn out to be a authorized resident of the state the place the service is positioned. Therefore, it is very important select one such as Florida or Texas where fees and taxes are a lot more cost effective than locations akin to New York or California.
What higher gift can you give one who is consistently on the street than this reward of having the ability to keep up a correspondence with house. Or, as soon as they arrive in a foreign country will enable them to arrange meetings, call up their contacts, make crucial reservations, examine their emails or browse for a lot wanted data. Nothing can certainly examine to this gift particularly if they don't have one but. Chances are they have already got one but this isn't a problem. Improve what they've.
You may also select to observe the ceremony with out participating. When you do, be sure that to maintain a respectful distance so that you don't impede the procession. In the event you take footage, do not use flash. Remember the fact that for the monks and locals, this is a crucial religious ceremony. So long as you might be respectful, this is usually a stunning and memorable custom to witness.
On our long vacation to my house nation, my hubby and I decided to deliver Angus, our Cavalier King Charles Spaniel dog with us. The Lug Life Nap Sac Blanket & Pillow is a comfortable carol fleece blanket with a matching zip pouch. June in all probability shall be pretty scorching, so April or early Could could be my recommendation. I had no thought that there were so many options for travel pillows! I love this lens.
F.C. Gorham Insurance presents a full vary of personal Insurance coverage, Enterprise Insurance and Monetary service merchandise. This means we will insure your property, private autos, enterprise autos, staff compensation, life insurance, enterprise property and legal responsibility Insurance coverage, private and commercial umbrella Insurance coverage, mortgages and investments.


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Victorian Rallying Strategic Plan
Rallying in Victoria is already facing, and set to face many more, issues that impact directly on the ongoing viability of the sport. Too often we are focused on dealing with the issues that are immediately before us, not looking ahead to proactively prepare for, and manage potential scenarios before they arise. Therefore, as Chair of the VRP, I (along with the other members) am embarking upon the development, and implementation, of this Strategic Plan for Victorian Rallying, looking at identifying and addressing the issues that will require our attention in the immediate future, and within a broader 5 year timeframe.
The strategic plan will examine the sport of Rallying in Victoria using the following categories as a framework:
- Event Management
- Course Checkers/Stewards
- Competitors
- Safety Requirements
- Junior Development
- Forest/Course Access
- Regulatory Issues
- Series Issues
- Calendaring of Events
- Vehicle Issues/Eligibility
Rallying in Victoria is going to be faced with an increasing array of challenges that will potentially threaten aspects of our sport, if not addressed in a timely, and responsible manner. In everything we investigate, and in the findings we present, the focus must remain on the ongoing sustainability and viability of Victorian Rallying, ensuring that our actions allow us to further develop and enhance the sport in the coming years. We are putting in the work towards planning now, in order to remain "ahead of the curve" well into the future.Download Strategic Plan


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To me that's not such an inspiring start to the year, so instead this January I made a list of goals instead. This list included personal as well as business aspirations, less a fixed 'to do' list and more a guide to start the year with a clear sense of direction.
A really great part of keeping this blogazine is the recording of this time in my life, a time-capsule of who I am now as an artist, designer and small business owner. I think it will be really interesting to look back on my aspirations in the future so I'm going to share a few of my goals here for posterity.
It's a long list I have to confess, so I will only share part of it!
- Get engaged.
- Work my butt off
I can only apologise to my darling friends in advance, I love you dearly but I will be thin on the ground this year.
- Make more things
Running a creative space can be really amazing, but sometimes I can get so busy enabling artists and designers to create and show their work to the world that I run out of time to do my own making. This year I'm making it a priority!
While we are on the subject I thought I would share some of the things I have been working on this summer. These things involve wood, brickies twine, foraged timber and the most wonderful pastel-flouro paint mix I have ever created.... it's ZINGY on the eyes!
Fun wooden goodies aren't the only thing I have been making this summer, I spent most of my Christmas holidays re-building our website and these pretty one-off wooden jewelry designs will be appearing on it soon. I'm really proud of the new site and would love to hear what you think of it too. You can see it here.
I know I don't always make it easy to respond to my ramblings and rants but I love to get emails from readers so please feel free to get in touch, My email addy is firstname.lastname@example.com you can also say 'hello' on our Facebook page too!
X Steph


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athens, ga or athens, greece?
I have extreme wanderlust and an overactive imagination, which are usually most distracting when a shitload of important, high-stress projects and exams is the only thing standing between me and summer vacation.
For example: What could be better than ignoring my responsibilities & pretending I'm lounging on the shores of a Greek island drinking ouzo and flirting with a handsome man named Kostos?! (Actually being there...? Fine, you're right.)
I also break into song even more than usual. Everyone has their own coping methods, ok?
Mid-daydream, I found this slightly see-through dress at Goodwill that I'm pretty sure is actually a nightgown and it just screamed Santorini to me.
Don't understand? Well you've obviously never seen Sisterhood of the Traveling Pants. I know, solely from this movie (and the sequel duh), that Santorini / the entire country of Greece is the most beautiful and magical place on earth.
And if you haven't seen Hercules I just don't know what you're doing with your life honestly.
So, because I have sadly not won the lottery or become an overnight blogging billionaire yet, I donned my Santorini-inspired outfits and settled for touring the most Greek-looking place I could find in Athens (Georgia).
Yes, I'm crazy, but I'm pretty sure it's more fun this way.
MOM: Ignore this whole post. I promise all I ever do is study and I made GREAT grades on all my finals ;)
"THE NUMBER OF GREAT PICTURES YOU'VE RUINED WITH YOUR EYES" -Catie of Stay Fierce Photography. she is obviously very fond of me.
Outfit 1 details: Old Navy sandals . Target crop top . American Eagle skirt . Alex & Ani Bangles . Cheap Sunglasses . Olives & Wax Vintage leather purse.
Outfit 2 details: Goodwill dress . Old Navy clutch . Gap leather belt .


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This blog will be for controversial or topical news and events in or around New Zealand, and certain promotional posts. Read my other blogs for regional interests.My forum for a rant.
Thursday, April 16, 2009
National's destiny is in Labour's hands...
Contary to opinions being expressed by editorials and other so-called political experts, National shows all the signs of a one term government - it hasn't really introduced any real rightwing philosophically influenced legislation, apart from the '90 Day hire and Fire Act'.
It is not National that is in control of its destiny, it is actually Labour! The power, the brains and the intestinal fortitude of Labour left parliament when Helen Clark and Michael Cullen decided to retire from politics and resign from the parliamentary Labour Party. It is shades of 1975, and 1990 all over again - a Labour Party without a real leader, not a disembelled Labour caucus though.
This Labour caucus will be back to business as usual with a longterm leader, which is not "Phil-in" Phil Goff!
Will there be another Helen Clark? Not during the remainder of my lifetime. Such people come along every couple of generations. The good people die in office; and the real leaders come along every fifty years or so. I don't include David Lange in that capacity - he comes under the capacity of orators. He had no intestinal fortitude to stand up to Roger Douglas; he could have promoted Helen clark as his deputy and taken on the Act Party's ancestors, Douglas and Prebble and Company!
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- January 7, 2010 at 2:48 PM
Good morning
We do not agree with this year Brit awards decision.
Please visit our little survey
Lady Gaga can not be better than Madonna
Poll supported by BRIT awards 2010 sponsor femmestyle
Pet Shop Boys surprise lucky BRITs fan with a MasterCard Priceless Gig in their living room
- March 9, 2010 at 5:57 AM


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6 Ways You Can Help Combat Climate Change
Regardless of the claims of some right-wing politicians and media shops, local weather change is a really actual menace to the long run survival of humankind on Earth. The deal agreed in November 2014 between the U.S. and China to cut back carbon emissions is a crucial step on the a part of influential political leaders to handle the difficulty. Nevertheless, the vested pursuits of the fossil gasoline business (and their sturdy ties and monetary backing of right-wing political events not solely within the U.S. but in addition the UK and Australia) and political in-fighting inside international locations signifies that a really world response to this most urgent of issues nonetheless appears a good distance off. Certainly, in Australia (a rustic, allow us to not neglect, that might be on the forefront of adjustments to the local weather, having already skilled drastic droughts and fires because of this – in accordance with the overwhelming majority of scientists – of world warning) the incumbent Liberal authorities has develop into the primary Western nation to really dismantle laws meant to fight the man-made warming of the planet by repealing a tax on carbon emissions.
Nevertheless, regardless of the irritating lack of accord among the many political lessons, local weather change remains to be being tackled on the person degree. Many individuals are taking it upon themselves to cut back their very own influence on world warming. Listed below are some ways in which everybody may help to fight local weather change.
Use vitality effectively
It sounds easy, however the easiest way to cut back emissions of the greenhouse gases which might be primarily accountable for local weather change is to cut back the quantity of vitality we use. The cumulative impact of small adjustments in vitality use may help scale back consumption. For example, within the house, flip home equipment off when you find yourself not utilizing them (moderately than placing them on standby), change gentle bulbs to low-energy alternate options, and solely run the washer when you've got a full load. Within the permaculture backyard you may also use planting strategies to switch temperatures in buildings, by establishing a deciduous tree subsequent to the home to supply shade, for instance.
Get inexperienced vitality
The overwhelming majority of the vitality we use is generated by the burning of fossil fuels. Nevertheless, there are a number of totally different strategies of producing vitality from renewable sources, specifically wind, photo voltaic, wave and geothermal. Growing numbers of firms are recognizing that buyers need to have the ability to entry inexperienced vitality, and you need to be capable to discover a inexperienced vitality provider in your native space to whom you'll be able to swap your vitality provide. Alternatively, you'll be able to set up inexperienced vitality seize programs in your property to develop into extra self-sustainable in your vitality use. Contemplate putting in photo voltaic panels or a wind turbine in case your property is appropriate. It's possible you'll even be capable to promote surplus vitality to the municipal system.
Use water effectively
It takes plenty of vitality to deal with, transfer and warmth water for home use, so utilizing it effectively inside your house is
water-21070_640one other approach of contributing to the battle in opposition to local weather change. Apart from growing water-aware habits, resembling turning off the faucet whilst you brush your tooth and solely filling the kettle with as a lot water as you want, ensure that all of your water home equipment, resembling bathrooms and showers, are in good working order to keep away from leaks and water wastage. As permaculturists, we search to maximise the effectiveness of irrigation within the backyard, and reusing family water, be it the liquid used to scrub greens or a full greywater recycling system, can considerably scale back water use.
Journey good
Each time potential, use public transport moderately than your automobile. Municipal mass transit programs are a lot much less polluting than automobiles, and by using them you point out your assist in your native authority to focus sources on enhancing and increasing them. For brief journeys, strolling will scale back your influence on the local weather to zero, and offer you an opportunity to train.
Typically, after all, you can't keep away from utilizing the automobile, however there are steps you'll be able to take to make sure your influence on the setting when behind the wheel is stored to a minimal. The very first thing is to ensure the car is operating at its most effective. Which means checking the elements of the engine are all in good working order, that the air filter just isn't blocked, that the oil is on the appropriate degree and is of the fitting grade, and that the tires are inflated to the perfect stress. These assist the automobile use much less gasoline, saving you cash and minimizing the necessity for repairs. As soon as on the transfer, keep away from harsh breaking and fast acceleration, and check out to not idle with the engine operating for prolonged durations of time.
You can even have a look at combining actions so that you solely make one journey within the automobile – by doing the grocery run on the way in which house from work, for instance, moderately than taking a separate journey – and car-sharing, notably you probably have colleagues on the workplace who dwell close to you.
Eat good
Scale back your meals miles – the vitality required to develop, harvest, course of, package deal and transport the meals merchandise you purchase – by buying native, seasonal wholefoods. Meals merchandise that haven't been processed with preservatives to allow them to be transported lengthy distances are additionally higher for you health-wise. Attempt to scale back the quantity of meat and diary you eat. Estimates put the proportion of greenhouse gases brought on by the manufacturing of meat and dairy merchandise at 18 %. Even going meat-free for simply sooner or later per week could have a big influence in your contribution to greenhouse gasoline manufacturing.
The vitality value to recycle supplies resembling paper, glass and plastic is way decrease than that required to fabricate new merchandise. Each time potential, use recycling schemes in your neighborhood, and if there isn't one, foyer the native authorities to institute such a scheme. Composting your meals waste reduces landfill and thus the greenhouse gases that leak from them. Deal with your belongings in order that they last more and also you shouldn't have to purchase new merchandise so recurrently.


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PhenQ is a dietary supplement included to the list of the world-beating anti-obesity products. These pills offer the multi-faceted approach to tackling the issue of excessive weight.
The product gives full play to the capabilities of the human body when it comes to getting rid of unwanted pounds.
Almost 200,000 customers have already had positive experience of using this product. And the popularity of these pills is gaining pace. It's the superior quality supplement produced with the use of FDA approved facilities.
The supplementation doesn't require any prescription.
Mechanism of Action
PhenQ precisely hits the target which is the excessive weight. It's possible due to the versatile mechanism of action of this product. It acts by:
- Reducing appetite. The supplementation suppresses your feeling of hunger, making you eat less. And the caloric intake will decrease.
- Speeding up metabolism. High metabolic rate is an obligatory condition for rapid weight loss since it's responsible for burning a surplus of calories.
- Destroying fat. The product helps burn extra calories due to the increased energy expenditure, fast metabolism, and thermogenic effect.
- Blocking fat storage process. The product formula discourages the body from storing fat. As a result, the body weight doesn't increase.
- Elevating energy levels. High energy levels will not allow you to feel exhausted and tired during the weight loss process. So, you will stay motivated and purpose-oriented.
The product efficacy is similar to the efficacy of several separate diet pills, each of which is focused only on one aspect of excessive weight problem.
Since the supplement manages the problem of excessive weight in a few different ways, you are sure to achieve your weight-loss goals.
How to Take
The supplement bottle contains 60 pills, which are very easy to take. It's necessary to use 2 pills per day. It will take a few months before you experience the first positive changes in your weight.
The supplementation is not associated with any health risks. There are no adverse effects and contraindications which can prevent you from using the product and enjoying all its benefits. Just follow all the recommendations on the label.
Does PhenQ Diet Pills Suit Vegans and Vegetarians?
PhenQ diet pills completely comply with the needs and requirements of both vegans and vegetarians. Regardless of your eating habits, this supplement will ensure aid in the achievement of your weight decrease goals.
Are There Any Restrictions to PhenQ Intake?
PhenQ s an absolutely safe anti-overweight product of natural origin that can be used by almost each adult with excessive weight. However, there are still some exceptions and restrictions.
Thus, people who didn't reach 18 years old, as well as pregnant and breastfeeding women shouldn't take PhenQ diet supplement.
The supplementation is also contraindicated to people who suffer from liver disorders, kidney disorders, endocrine system diseases, diabetes, autoimmune diseases, breast cancer, testicular cancer, prostate hypertrophy, and depression.
In addition, during the intake of this pill you shouldn't use anti-depressants or any other medications.
Supplementation Benefits
Undergoing PhenQ supplementation, it's possible to:
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PhenQ is not a strong synthetic medication. On the contrary, it's the all-natural anti-obesity remedy which doesn't contain any synthetic active substances. That's why you will not lose your weight instantly.
The supplementation will bring maximum effect if you combine it with a well-thought out diet plan, professional workout program, and positive lifestyle changes. If you really do this, it will take from 2 to 4 months to decrease the body fat percentage by 15%. The results depend on the efforts you make.
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PhenQ is a trustworthy, safe appetite suppressant and fat burner that has already earned an excellent reputation among the customers.
When purchasing it, you get one top-notch non-prescription weight-loss supplement with multiple favorable effects.
You pay only once instead of paying for several products to get similar efficacy. It means that you not only have an opportunity to undergo high-quality dietary supplementation, but also can save money.


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An endogenous microRNA (miRNA1166.1) can regulate photobio-H2 production in eukaryotic green alga Chlamydomonas reinhardtii
- Yuting Wang†1,
- Xiaoshan Zhuang†1, 2,
- Meirong Chen1, 2,
- Zhiyong Zeng1, 2,
- Xiaoqi Cai1, 2,
- Hui Li1, 2Email author and
- Zhangli Hu1, 2Email author
© The Author(s) 2018
- Received: 7 February 2018
- Accepted: 20 April 2018
- Published: 2 May 2018
Hydrogen photoproduction from green microalgae is regarded as a promising alternative solution for energy problems. However, the simultaneous oxygen evolution from microalgae can prevent continuous hydrogen production due to the hypersensitivity of hydrogenases to oxygen. Sulfur deprivation can extend the duration of algal hydrogen production, but it is uneconomical to alternately culture algal cells in sulfur-sufficient and sulfur-deprived media.
In this study, we developed a novel way to simulate sulfur-deprivation treatment while constantly maintaining microalgal cells in sulfur-sufficient culture medium by overexpressing an endogenous microRNA (miR1166.1). Based on our previous RNA-seq analysis in the model green alga Chlamydomonas reinhardtii, three endogenous miRNAs responsive to sulfur deprivation (cre-miR1166.1, cre-miR1150.3, and cre-miR1158) were selected. Heat-inducible expression vectors containing the selected miRNAs were constructed and transformed into C. reinhardtii. Comparison of H2 production following heat induction in the three transgenic strains and untransformed control group identified miR1166.1 as the best candidate for H2 production regulation. Moreover, enhanced photobio-H2 production was observed with repeated induction of miR1166.1 expression.
This study is the first to identify a physiological function of endogenous miR1166.1 and to show that a natural miRNA can regulate hydrogen photoproduction in the unicellular model organism C. reinhardtii.
- Biohydrogen
- Green alga
- Chlamydomonas reinhardtii
- microRNA
- Gene regulation
Hydrogen gas (H2) is a clean energy source, and hydrogen production from green algae is regarded as a promising alternative solution for energy problems, since green algae have iron hydrogenases with high enzyme activity and can rely on solar energy for growth [1–8]. However, hydrogen photoproduction in green algae lasts for just a few seconds to a few minutes due to the hypersensitivity of iron hydrogenases to simultaneously generate oxygen from photosynthesis. Moreover, algal cells only produce hydrogen with excess electrons from the photosynthetic chain to prevent overreduction [9–12]; thus, the critical problem restricting the application of hydrogen production by green algae is the unsustainability of hydrogen biosynthesis.
A conventional sustainable hydrogen production method depends on the reversible inactivation of oxygen evolution through two-stage algal cultivation [13–15]. The first stage is oxygenic photosynthesis to accumulate algal biomass, and the second stage is anaerobic hydrogen production in a sulfur-deprived medium. Under sulfur deprivation, algal cell respiration exhausts oxygen and causes anaerobiosis in the culture. Therefore, this two-stage method separates hydrogen production from oxygen evolution and carbon accumulation, and sulfur deprivation makes sustained hydrogen production possible [11]. However, the replacement of normal medium with sulfur-deprived medium requires the centrifugation of algal cells, which may affect cell activity and is difficult to apply in large-scale production.
Comparative studies of sulfur-deprived and sulfur-replete algae have been conducted at different molecular levels in Chlamydomonas reinhardtii [13–20]. A better understanding of the molecular mechanism of sulfur-deprived stress response may pave new ways for genetic regulation to improve algal photobio-H2 production. Previous results showed that sulfur-deprived cells had massive changes in cellular physiology and metabolism, along with the accumulation of proteins with fewer sulfur-containing amino acids [17]. Microarray analyses showed that photosynthetic genes, one exception being LHCBM9, are generally repressed by sulfur deprivation, suggesting a major remodeling of the photosystem II light-harvesting complex [16]. Sulfur deprivation also affects proteins related to photosynthetic machinery, protein biosynthetic apparatuses, molecular chaperones, and 20S proteasomal components [17]. Recently, we found that non-coding RNAs, including microRNAs (miRNAs), which function as important gene regulators, are also significantly influenced by sulfur deprivation [18, 19]. Further analysis suggested that miRNAs are potentially involved in photobio-H2 production in C. reinhardtii.
miRNAs are a class of endogenous non-coding small RNAs in eukaryotes. They are approximately 18–24 nucleotide (nt) in length and processed from RNAs into stem–loop precursor structures by the nuclease Dicer. miRNAs regulate target gene expression by promoting mRNA degradation and/or inhibiting protein translation. Since miRNAs are present in the unicellular green alga C. reinhardtii, the miRNA pathway represents an ancient gene regulation mechanism that evolved prior to the emergence of multicellularity [21, 22]. In multicellular organisms, miRNAs have been shown to be an important and widespread regulator. Animal miRNAs are involved in developmental timing, cell death, cell proliferation, and patterning of the nervous system [23, 24]. In fact, about one-third of human genes may be miRNA targets [25]. miRNAs are associated with the development of diseases including cancer [26, 27], and they regulate a range of developmental and physiological processes, such as hematopoiesis [28]. In plants, miRNAs also play dominant roles in post-transcriptional gene regulation and modulate organ development, phase transition, and stress responses [29]. In contrast, the functions of miRNAs in unicellular eukaryotic algae remain largely unknown [30]. In C. reinhardtii, some miRNAs (Cre-miR914 and Cre-miR910) were found related to multiple stresses (heat shock, UV-B, and salinity) [31], but functions of most miRNAs still haven't been studied.
In this study, three endogenous miRNAs responsive to sulfur deprivation (cre-miR1166.1, cre-miR1150.3, and cre-miR1158) were selected from a previous RNA-seq database in the green alga C. reinhardtii. Heat-inducible miRNA overexpression transformants were constructed. By comparing H2 production following heat induction in the three transgenic strains and the WT group, miR1166.1 was identified as the best candidate for H2 production regulation. Moreover, the enhanced photobio-H2 production of miR1166.1-overexpressing algae could be repeatedly induced. To our knowledge, this is the first report of an endogenous miRNA affecting photobio-H2 production in C. reinhardtii.
Algal strains and culture conditions
The cell-wall-deficient C. reinhardtii strain CC-849 served as the receptor strain and negative control. Algal cells were cultured in TAP (Tris–acetate-phosphate) medium at 22 °C under continuous cool-white light. Sulfur-deprivation medium (TAP-S) was prepared by replacing the S-salts with their chloride counterparts. For 1 L of TAP-S medium, 40× Filner's Beijernicks Solution (25 mL), 1 M potassium phosphate (1 mL), trace mineral solution (1 mL), and Tris base (2.42 g) were combined, and the pH was adjusted to 7.0 with glacial acetic acid. For sulfur-deprivation treatment, 400 mL cells at exponential phase were collected by centrifugation then washed twice with liquid TAP-S medium. Equal quantities of algal cells were resuspended in TAP and TAP-S media and grown for up to 72 h under continuous white light illumination (≈ 20 μmol photons m−2 s−1). The cells were collected for RNA isolation, and the sulfate concentration in the supernatant was detected using a Dionex ICS-1100 ion chromatograph [4, 15].
Sequencing of miRNA
Total RNA was extracted using Trizol reagent (Invitrogen) according to the manufacturer's protocol, and RNA quality was examined on an Agilent 2100 Bioanalyzer. For library construction, small RNAs were purified by PAGE and ligated to adaptors. Sequencing of the two libraries was performed using Illumina Solexa sequencing. Raw reads were filtered and compared to the miRBase database (release 15.0). Further details can be found in our previous work [18].
Construction of miRNA overexpression vectors
The pH124 vector, which contains the ble gene for zeocin resistance, was used as the expression vector [32–35]. An artificial miRNA precursor was introduced into pH124 to permit inducibility by high heat shock treatment in C. reinhardtii. The artificial miRNA precursor was based on the backbone of the highly expressed cre-miR1162 precursor [36–38]. The constructed artificial miRNA precursor sequences were synthesized in vitro, and the NheI (GCTAGC) and PmaCI (CACGTG) digestion sites were used to insert the sequences into pH124. The construct containing the promoter, miRNA precursor, and ble gene integrated randomly into the alga nuclear genome.
Genomic DNA PCR analysis
PCR was performed to verify the transformation of the artificial miRNA precursor constructs into algal cells. PCR was performed using the primer pair 593-F (5′-TGACCTCCACTTTCAGCGACA-3′) and 593-R (5′-ACTTGAGAGCAGTATCTTCCATCCA-3′), which map to the region outside of the multiple cloning site in the pH124 vector. The PCR cycling conditions were as follows: pre-denaturation at 94 °C for 4 min; 30 amplification cycles of 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 60 s; and a final extension for 10 min at 72 °C. All amplification products were verified by sequencing and alignment analysis.
Hydrogen detection
Equivalent culture volumes of CC-849 and the transgenic algal strains (400 mL) were cultured in 500 ml bottles sealed with rubber sheet septa until exponential phase. The cultures were pre-treated in the dark for 24 h then cultivated under continuous light to detect gas contents. Gas samples (1 mL sample volume) from the headspace of the cultures were drawn with a syringe and separated using a molecular sieve column (length 6 ft, 1/8 in. OD, 2 mm ID, MolSieve 5A packing, mesh size 60/80). A gas chromatograph with a thermal conductivity detector was used to detect the concentration of H2 (Agilent 7890A; Agilent Technologies Inc., USA). Argon was used as the carrier gas [4]. To test the sustained hydrogen production capacity (Fig. 6), we used triplicates for each strain, and cultured them with the same initial cell density under the same condition. Algal cells at exponential phase were treated in 40 °C water incubates for 1 h and then recovered to 22 °C. After 3, 5, 7, and 9 h of recovery, these cells were heated for the second time and recovered for 3, 5, and 7 h before the heat and recovery were repeated for the third time. H2 concentrations were measured respectively at the mentioned times.
Small RNA extraction
Small RNAs were isolated from both CC-849 and transgenic algae using the E.Z.N.A.® miRNA Kit (OMEGA) according to the manufacturer's protocol. Polyadenylation was performed using the Poly (A) Tailing Kit (Takara) according to manufacturer's instructions. The small RNAs were reverse transcribed using poly (T) adapter (Additional file 1: Table S1) according to the S-Poly(T) method [39].
Quantitative real-time PCR
Quantitative real-time PCR (qRT-PCR) was used to measure changes in miR1166.1 expression in CC-849 and transgenic algae. Quantitative real-time PCR was performed using SYBR Premix Ex TaqTM II (Takara, Japan) according to the manufacturer's instructions on an Applied Biosystems 7300 Real-Time PCR System (Framingham, MA, USA). Quantitative real-time PCR was performed using a universal reverse primer and miR1166.1-specific and U4-specific forward primers [39]. The primer sequences are listed in Additional file 1: Table S1. The PCR cycling conditions were as follows: pre-denaturation at 95 °C for 30 s, followed by 40 two-step cycles of 95 °C for 10 s and 60 °C for 30 s. U4 snRNA was used as the reference gene for the quantitative real-time PCR detection of miR1166.1. The data were analyzed using the 2−ΔΔCt calculation method.
Identification of miRNAs potentially involved in C. reinhardtii photobio-H2 production
Construction of miRNA overexpression transgenic algae
Selection of high H2 production transgenic algal strains
Characteristics and secondary structure of miR1166.1
Quantitative real-time PCR analysis of miR1166.1
Enhanced H2 production of transgenic algae
miRNA functions have been studied extensively in animals and plants, but much has yet to be uncovered about the physiological functions of endogenous miRNAs in the unicellular model organism C. reinhardtii. Firstly, there are few studies providing foundational information about non-coding RNAs in this green alga. Additionally, phenotypic assessment of genetic mutants to functionally characterize miRNAs, a powerful approach in plants, is uncommon in microalgae because their phenotypes are difficult to observe. In plants, miRNA regulation affects plant development and morphology, and plant phenotypes can be very informative about miRNA functions and expression levels [29, 37]. In C. reinhardtii, a few miRNAs have been confirmed to cleave mRNAs or repress mRNA translation, but no significant phenotypes attributable to miRNA alteration have been reported. The present findings link the phenotype of enhanced hydrogen production with a natural miRNA in the unicellular microalga C. reinhardtii, thereby identifying a physiological function of this miRNA.
Sulfur deprivation induces sustained hydrogen production in C. reinhardtii [13], and we found that miR1166.1 was significantly up-regulated after sulfur deprivation. We therefore hypothesized that miR1166.1 might be a hydrogen biosynthesis regulating factor. The hydrogen yield data from the heat-inducible miR1166.1 transgenic algal strain confirmed that miR1166.1 enhanced hydrogen biosynthesis and that the high transcription level of miR1166.1 led to the increase in hydrogen yield. This is the first evidence that a natural miRNA in green algae regulates hydrogen photoproduction.
miRNAs in C. reinhardtii were considered to play a limited role in responses to nutrient deprivation, including sulfur or phosphate deprivation [40]. In general, the miRNA overexpression transgenic strains usually don't have lethal phenotype or significantly growth phenotypes according to our experience. In this study, our result shows that endogenous miRNA1166.1 significantly affects H2 production in C. reinhardtii. We propose that, compared to a signal miRNA like miRNA1166.1, multiple miRNA regulation may have more significant effect. What's more, the miRNA target prediction in C. reinhardtii should be different from that in higher plants and animals, with the rather complicated identification of miRNA target genes, thus the results obtained from predicted targets may not be able to represent the function of miRNAs accurately. More studies on C. reinhardtii miRNA functions are still necessary.
We established an efficient heat-inducible system based on the regulatory function of a natural miRNA to control sustained hydrogen production in green algae. The miRNA transcription level (Fig. 5) and hydrogen yield (Fig. 6) showed that the baseline expression of miR1166.1 in the transgenic algal strain was slightly higher than in CC-849. This is probably because the HSP70-RBCS2 promoter can be induced by light as well as heat [4, 32]. However, heat treatment had a stronger effect and could be applied repeatedly to achieve sustained hydrogen production.
We performed qPCR and sequencing to investigate the transcription levels of the miRNA. The result showed that the artificial miRNA precursor had been successfully cleaved to yield the endogenous mature miR1166.1 sequence. Previous studies have demonstrated that the miR1162 backbone is a powerful tool for expressing artificial miRNA sequences [4, 36]. This study further shows that the miR1162 backbone is also applicable for the transgenic expression of natural miRNA sequences.
Target gene prediction of miR1166.1 was performed to investigate the molecular mechanism of this miRNA, but the verification of miRNA target genes is difficult and considerable. Unlike the typically extensive complementarity of miRNAs to their targets in land plants, complementarity to the miRNA seed region is sufficient to induce target gene repression in C. reinhardtii [38]. This difference leads to a broader range of miRNA target genes and a higher probability of inaccurate prediction, consequently making miRNA target identification in C. reinhardtii more complicated. The investigation of the target genes of endogenous miR1166.1 is currently ongoing in our laboratory.
miRNAs in the unicellular model organism C. reinhardtii were initially reported in 2007, and there have been a few studies about the functional mechanisms of miRNAs in this species. However, the present study is the first to identify the physiological function of a natural miRNA in C. reinhardtii. Moreover, natural miR1166.1 regulates hydrogen photoproduction, which is considered a promising strategy for solving energy problems. We previously found that an artificial miRNA targeting OEE2 could enhance hydrogen production; here, we demonstrated that a natural miRNA could also be a hydrogen photoproduction regulator. The findings may provide a novel approach for improving hydrogen production without medium replacement.
Authors' contributions
ZH designed and guided the study. HL participated in study design and manuscript writing. YW participated in manuscript writing and performed the statistical analysis. XZ performed vector construction and positive transformant identification. MC participated in primer design and performed quantitative real-time PCR. XC carried out the gas detection. All authors read and approved the final manuscript.
Competing interests
The authors declare that they have no competing interests.
Availability of data and materials
All data generated or analyzed during this study are included in this published article and its Additional files.
Ethics approval and consent to participate
Not applicable.
This work was supported by the National Natural Science Foundation of China (31470431), Guangdong Natural Science Foundation (2014A030308017 and 2016A030313052), Project of DEGP (2015KTSCX125), Shenzhen Grant Plan for Science & Technology (Shenzhen basic research projects, Subject layout project), Demonstration Project for Marine Economic Development in Shenzhen (2017 documents No.283, Department of the Science and Technology, China's State Oceanic Administration) and Shenzhen special funds for Bio-industry development (NYSW20140327010012).
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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Final Score: Tampa Spartans 75, Rollins Tars 62
Records: Tampa (9-5, 3-3 SSC), Rollins (8-7, 2-5 SSC)
Location: Martinez Center || Tampa, Fla.
All-Time Series: Tampa now holds a 66-36 advantage in the all-time series, which dates back to 1961. This is UT's second straight win over the Tars.
1st Half | Rollins would net the first basket of the game, which would go down as the lone lead of the night for the Tars. The Spartan lead would reach double figures at the mid-way point of the first half. Tampa would then outscore the Tars 12-6 over the final five minutes of the half to take their largest advantage of the game to the break at 38-23.
2nd Half | Tampa would hold its significant lead for the remainder of the game, leading by as many as 19 points. The Tars were only able to get as close as nine points. UT would hold the Tars to just two points in the final three minutes of the contest as they pulled away for the league win.
- Vincent Biffle was the top scorer with 23 points as the junior from Indianapolis was 6-for-7 from the free throw line.
- Senior Anthony Gamble added 22 points while connecting on three from beyond the arc.
- Cedric Belemene pulled down a team-leading 14 rebounds as he registered his fourth double-double of the season with 13 points.
- Freddy Ibrahim tallied a career-best 10 assists as he also matched Biffle for team honors with two steals.
- Despite being outshot 46.4 to 42.6 percent from the field, UT connected on 15 free throws compared to just three for the Tars.
- Tampa won the battle of the boards with 41 rebounds compared to 27 for the visitors. Of the 41 boards, 16 were on the offensive end.
- Tampa has now won five of its last six games.
- UT is now 5-2 at the Martinez Center this season.
- The game featured a pair of 700-plus win coaches in UT head coach Richard Schmidt (704) and Rollins' Tom Klusman (701).
UP NEXT: Tampa faces a stiff road test as it travels to Fort Lauderdale for a meeting with No. 4 Nova Southeastern on Saturday at 4 p.m.
Follow UT Athletics: @TampaSpartans on Twitter, Facebook, Instagram, Snapchat and YouTube.
Text Alerts: UT also offers a free text messaging service that sends text alerts following all Spartan events. To sign up, please register at www.tampaspartans.com.


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Each person who survives being raped experiences and processes the trauma of that event in different ways. For some, the effects of that experience can last a lifetime. For others, the aftereffects can be short term. The mental and emotional effects of rape should not be ignored after the physical signs and symptoms of the attack have resolved. Seeking counseling after a rape is crucial. However, many victims choose to ignore or disassociate from what happened. Avoiding the resulting trauma from a rape can lead to significant and severe mental health issues later on. If you need an affordable counselor in Charlotte, NC, that you can trust, call Legacy Freedom for help.
Here is what you need to know about the most common mental and emotional effects of rape:
- Dissociation - It is common after a physical attack for the victim to experience an inability to concentrate on schoolwork or tasks at work. Struggling with feeling present in everyday situations is common as well.
- Post-Traumatic Stress Disorder (PTSD) - Having nightmares, anxiety, flashbacks or racing thoughts are signs of PTSD.
- Depression - Withdrawing from friends, family members or partners is common after an attack. Suicidal thoughts or feelings may develop and should be treated as serious. Immediate attention from a professional should be sought.
- Intense Feelings of Guilt - Many victims will blame themselves for the attack and develop a sense of unworthiness or express that they feel damaged.
- Self-Medication - Using alcohol, drugs or food to cope with the feelings or trauma related to the attack. Excessive substance abuse and engaging in risky behaviors are common.
- Severe Anxiety - Chronic panic attacks, avoiding social situations, experiencing hypervigilance, and feeling unable to leave home are common.
- Explosive Anger - Outbursts of rage or anger when the event is mentioned or when they are confronted with new memories about the incident.
Sexual violence is never the fault of the victim. Understanding the definition of sexual assault is crucial for both victims and their loved ones. Sexual assault or rape is defined as fondling, unwanted touching, forcing the victim to perform penetration on the perpetrator or oral sex and penetration of the victim's body. This is not limited to just physical force. Force of any kind including threats, coercion, manipulation or action without consent are all considered sexual assault.
According to the Rape, Abuse & Incest National Network (RAINN), one in six women and one in thirty-three men experience a completed or attempted rape in their lifetime. By coming forward and reporting rapes and sexual assaults, victims can help remove the stigma of shame and guilt associated with being a rape victim.
Affordable Counselor in Charlotte, NC You Can Depend On
At Legacy Freedom of Charlotte, we understand that talking about what happened to you isn't easy. We know you want to put the incident behind you and take back your life. Through our holistic approach to mental health care we can help you overcome your PTSD, anxiety, depression or any other co-occurring mental health issues and restore your sense of safety and security. Together with your care team you will create a plan that focuses on you, not your mental health needs. Helping you heal, mind, body, and spirit is crucial to your long-term success and well-being.
Our holistic approach utilizes traditional talk therapy in both one-on-one and group formats as well as access to a dietician and physical therapist to help you fuel your body through proper nutrition and exercise. Alternative therapies like equine therapy, acupuncture, and EMDR help you confront your trauma and then learn to set healthy boundaries and work through the memories of the attack.
Call or click to connect today with a highly trained anxiety counselor in Charlotte, NC!


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The pursuit of world domination isn't the U.S.' unique selling point. It's their success.
I guess, the U.S.* are doing a pretty good job at world** domination.
*Of course, it is not "the U.S.", but "the U.S. 1%".
**And it is not "world", it is "world, including 99% of U.S.' citizens".***
***Now I'm being watched by the three-letter-agencies**** more closely.
****G-O-O-G-L-E ... well, that one just counts twice.


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Did you know that every full-time staff member in Residence Life & Housing holds a masters degree or higher? Residence Life & Housing creates a home away from home in the residence halls for our residents. Our staff serves as a helpful resource for all residents and ensures that living on campus is an inclusive, safe, and fun living and learning environment for all.
Who are the Residence Life & Housing Staff?
Assistant Vice President for Residence Life & Housing: Dr. Sarah Mansfield, Ed.D
Dr. Mansfield joined Stevenson University in 2011 and is responsible for overseeing the on-campus living experience and for providing a safe and community-based living and learning environment in the residence halls.
Assistant Director for Housing Operations: Evan Huckfeldt, M.Ed
Evan Huckfeldt joined Stevenson University in 2015 and is responsible for overseeing the housing operations procedures of the residence halls including: housing assignments, meal plan assignments, residence halls opening, residence halls closing, key management and oversight of the department front desk.
Resident Director for the First Year Area: Jason Summers, M.Ed
Jason Summers joined Stevenson University in 2016 and is the Resident Director for the first year area. In addition to overseeing the first year area, Jason assists with the Office of Multicultural Experience to provide a variety of inclusive programming.
Resident Director for the Upper Class Suites: Elspeth Peterson, M.S
Elspeth Peterson joined Stevenson University in 2017 and is the Resident Director for the upper class suites. In addition to overseeing the upper class suites, Elspeth assists with housing operation and student leadership initiatives.
Resident Director for the Apartments: Patrick Murnane, M.A
Patrick Murnane joined Stevenson University in 2017 and is the Resident Director for the apartment complex. In addition to overseeing the apartment complex, Patrick coordinates our Resident Assistant staff selection and training.
Resident Directors are full-time professional, live-in staff members. Resident Directors assist with problems, supervise Resident Assistants, encourage student development and a direct resource for residents.
In addition, we have student leaders, Resident Assistants, who are student leaders who live in the buildings and help make students experience in the halls the best it can be. They are carefully selected and specially trained to help in case of emergencies, roommate issues, or concerns.
Residence Life staff members are on-duty every night during the regular academic year.


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Pumpkins are something of a natural two-for-one deal. Whether you use them to make jack-o-lanterns or pie, all pumpkins contain a valuable prize in their pulpy core -- nutritious, edible seeds. Also known as pepitas, pumpkin seeds are dark green, flat, mildly sweet and tender, much like the seeds of other kinds of winter squash. Although some pumpkin varieties produce seeds without shells, most pumpkin seeds are encased in a cream-colored shell, or hull, that is just as edible as the kernel it protects.
Pumpkin Seed Kernels
Shelled pumpkin seeds, or kernels, are a mineral-rich source of unsaturated fatty acids and quality plant protein. A 1-ounce serving of pumpkin seed kernels has right around 160 calories, 14 grams of mostly unsaturated fat, 8 grams of protein, 2 grams of carbohydrates and almost 2 grams of dietary fiber. A serving also provides nearly 40 percent of the recommended daily value for magnesium and just over 30 percent of the recommended daily value for phosphorus, based on a 2,000-calorie diet. Pumpkin seed kernels are a good source of iron, zinc and niacin, too.
Shelled vs. Whole
Pumpkin seed shells get their chewiness from fiber, which is largely what makes whole seeds nutritionally different from the shelled variety. While the kernels are mostly fat and protein, the shells are mostly carbohydrates. For this reason, whole pumpkin seeds are approximately 20 percent lower in calories and 60 percent higher in fiber than the shelled variety. You'll get about 125 calories, almost 6 grams of fat, 5 grams of protein, 10 grams of carbohydrates and 5 grams of dietary fiber from a 1-ounce serving of whole, roasted seeds. Although the shell itself isn't a significant source of vitamins and minerals, it is an excellent source of zinc -- whole pumpkin seeds supply about 25 percent more zinc per serving than shelled seeds.
Basic Preparation
You can eat whole seeds straight from the pumpkin, once you separate them from the pulp and rinse off the sticky residue. To dry the seeds quickly, spread them in a single layer on a baking sheet and set them in a 250-degree oven for about 20 minutes. Toss them with oil and spices, and roast them at the same temperature for another 20 minutes, cool and enjoy. If you prefer to eat just the kernels, spread the seeds on a sheet of parchment paper and use a rolling pin to crack the shells. In a pot of boiling water, the cracked shells will open and release their kernels. Discard the shells, rinse the kernels and let them dry.
Storage Considerations
Because of their high oil content, pumpkin seeds -- like all nuts and seeds -- are prone to spoil quickly. Whether you buy them in a package or from a bulk bin, avoid pumpkin seeds with cracked shells. With or without the shell, pumpkin seeds should be kept in an airtight container and stored in the refrigerator, unless you plan to eat them soon after buying them. Pumpkin seeds generally keep for two to six months in the refrigerator. If they smell like mildew or oil paint, chances are they're rancid and should be discarded.
- USDA National Nutrient Database: Seeds, Pumpkin and Squash Seeds, Whole, Roasted, Without Salt
- USDA National Nutrient Database: Seeds, Pumpkin and Squash Seed Kernels, Roasted, Without Salt
- U.S. Food and Drug Administration: Calculate the Percent Daily Value for the Appropriate Nutrients
- Wellness Foods A to Z: An Indispensable Guide for Health-Conscious Food Lovers; Sheldon Margen, M.D.
- Encyclopedia of Healing Foods; Michael Murray, N.D., et al.


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Tail Light Left 632010 - Mini (63-21-7-435-135)
This product is a genuine OEM BMW part with a warranty.
- 63-21-7-435-135
Union Jack tail light for F56 Minis, 3-18 and later. Needs to have extended led option.
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To sweeten the Bangkok pot for Menu For Hope III I'm adding more to my offer. Thanks to the generous folks at Bed Supperclub:
(image courtesy of mihobsen)
I'm able to throw in a meal for two (Sun-Thurs) at the above, one of Bangkok's hottest tables. And yes, just in case you thought you misheard me, this is being offered along with a six-hour tour of Bangkok's foodiest sites:
including the above; a route custom-designed by myself. The code for both of these is AP44.
You have until the 22nd. Buy as many of the $10 tickets as you can afford here.


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FNZ UK and Europe chief executive Martin Jennings is to leave before 2016.
Jennings was previously managing director of the Axa Elevate platform, and joined the technology firm in May 2011 to serve as chief operating officer for Australia and New Zealand, later becoming UK and Europe chief executive in December 2013.
Jennings' responsibilties will be taken on by global chief executive Adrian Durham.
An FNZ spokesman says the firm is seeking to reorganise to better align staff on functional, rather than regional, lines.
"Martin has made a significant contribution to FNZ over the past fours years in various roles, and FNZ wishes him all the best for the future," he says.
FNZ provides platform technology to a number of financial services giants including Axa, Friends Life, Standard Life and Zurich.


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Opening a bottle of his own Champagne Hommage, Claude Giraud, owner of Champagne Henri Giraud, smiles and pours some of Houston's most exceptional champagne connoisseurs a glass. Jane Gajano Blythe, co-founder of Boutique Wine Brokers, translates his French to English.
Dinning with Executive Chef Ryan Hildebrand at Triniti Restaurant on Shepherd in Houston last week, Claude Giraud discusses with wine enthusiast his work on the idea of wine's relationship with wooden casks, sourcing the wood from local forests.
The Giraud family have been on the forefront of wine making dating back to the 17th century in Ay, France. Claude Giraud spent the past 25 years researching the ageing of wine in oak barrels. Claude's research concludes that the geology of the soil influences the character of the oak as well as the vine itself. The Argonne Forest and the soil it sits on, also referred to as the 'terrior', is considered a key ingredient in wine making. Claude comments, "The wood and champagne relationship has to be perfect. We have done a lot of research with the forest, choosing the specific trees from the Argonne forest to make casks. We have introduced a new method of identification in the history of wine and Champagne making."
The 21st and 22nd of April Giraud will officially reveal the results of 25 years of research on oak cask winemaking and the link between terroir of the vines and the forest land itself. Renowned wine professionals have been invited to trail walk and picnic in the Argonne Forest. VitaBella, Luxury Wine Marketing, reports, "The event will be used to launch the patronage operation with the French Forestry Commission (ONF). Every Argonne Cuvée bottle sold will go towards replanting of the forest."
You can find more information about Champagne Henri Giraud here


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Sprint HTC Hero :: How To Get Track Ball To Light Up?Feb 9, 2010
So a guy I work with has a Droid Eris and the ball will light up when he has a missed call or a text. Is there a way to get the Hero (sprint) to do this? Also it seems the phone has two lights on the front? I have only seen the red/green light, light up.View 19 Replies


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PENELOPE | Floral Small Wedding Comb with Leaf and Pearl Accent - Available in Gold or Silver
SAMPLE SALE IN GOLD OR SILVER - This hair comb has been used once for a photo shooting only. It is in great condition although not as bright gold as if it was brand new. The silver comb is brand new.
The perfect definition of the botanicals, the Penelope hair comb was designed for the bride with a love for all things floral.
Featuring detailed gilded leaves and flowers finished with a pearl, this hair comb is the ideal hair piece for the modern bride seeking to add a vintage touch to her bridal look.
Compact and light, the Penelope comb is the kind of piece that suits all kinds of hairstyles. Though we love it placed above the ear with a side swept hairstyle, it complements loose buns and is a delight when combined with low back gowns and boho-inspired dresses.
Not only for brides, this dainty hair comb could be the ideal gift to your bridesmaids or dearest ones for any special occasion.
- Designed and handmade in Sydney, Australia
- Features brass elements, Swarovski pearls
- Tarnish resistant jewelry wire, plated hair comb
- 4,5x2,5cm at widest points
- Made to order
This hair piece is made to order. Please allow 3-5 days for production plus delivery times.
Shipping + Delivery Time
We offer free economy shipping worldwide. Shipping upgrades are available at the checkout for a fee.
Rush Order
If your order is time critical, please contact us at email@example.com before you submit your order and we can confirm timeframes. We do not charge extras for rush orders but please be reasonable and give us your exact wedding date or moving date so we know if expediting the production really is necessary.
Return + Exchange
Due to the delicate nature of each piece, we are unable to accept refunds or exchanges for a change or mind after an item has been put into production or made
Fore more information, please check our Policy and FAQ


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yi yi
If the y-axis flip is required, the rotation functions become
x2 yi y2 xi
FIGURE 11 Rotation of coordinate system.
In our approach rotation functions without flip are defined as
The orientation correction function is applied for CR images only. Other modalities do not require this type of standardization. The acquisition stations themselves secure a correct image orientation.
FIGURE 11 Rotation of coordinate system.


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Wednesday, November 27, 2013
Wondering to the funeral home.
I just don't have anything else to say, except that you all have a great day, you hear?
7 comments:
Sorry to hear that. Hope the rest of the family gets well soon, so sad.ReplyDeleteReplies
Thanks Jim, we all hope so, too. His dad has that lung disease from handling asbestos.Delete
How sad Dizzy-Dick. Moreso, when people are celebrating Thanksgiving and it's also coming up to the Christmas season. I'm sure you was a source of strength for them and my condolences to all the family.ReplyDelete
I know your wife likes to make cards, so why not put them on your list, and start to send them lovely encouraging cards every now and again. Just a thought.Replies
We will try to do what ever we can.Delete
sending up prayers to lift you up and the family who have lost a love one.ReplyDelete
God bless all of you,
It was only a month ago when my Aunt (the last of that generation) passed away. I guess it is right that the only thing that is for sure is death and taxes.Delete
oh, my goodness, Dizzy... I missed this post... how terribly sad! good lord! and you're right .... the only thing we can be sure of is death and taxes ... but armed with that knowledge, it still hurts...ReplyDelete


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Jul 29, 2017
Favorite baby wipes?
Possibly an odd question - but parents, do you have a favorite brand of baby wipes here? We have spent most of our 4 years in Japan living on an American military base so whilst we bought Japanese brand diapers we usually bought American wipes. I find that the baby wipes I've tried in Japan so far don't have the same textured feel to them like American ones. I'd love to hear about your favorite wipes brand and WHY they're the best choice for you and your little ones! Thanks!
After spending the last several years in the beating heart of Tokyo, I will be spending the next three in the countryside of Japan. I adore this country and all it has to offer - and I'm always learning more and more about life here as I go along!
5 Answers
Best Answer
Hands down: Moony Cashmere wipes. Over the years I tried a number of wipes with my four kids. The Moony Cashmere wipes were the only ones that didn't cause my kids irritation and they very rarely had nappy rash. The only time one of them had nappy rash is when I used another brand for a longer period! When my friend came to visit last year she was using Irish wipes on her boy until she ran out. I gave her the Moony to try and she (and the baby!) loved them, because they are mainly water so you don't have to worry about chemicals. I am a loyal dedicated Moony wipe fan!!


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08 Jan 6 Astounding Photos of HDB BTO 5-Room At Acacia Breeze That You Need To See Now
Posted at 05:57h in The Home Owners PrideOne of the many aspects of a modern urban home is the pop of color it can incorporate into its interiors or overall design theme. This is evident in the living and dining rooms of this HDB BTO apartment. Both areas share one big space making them sync with each other. Two lazy boy chairs are the best furnishings for the living area while the colorful chairs.


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A. has a notion that he might like to go to the "live-model drawing class" that they host at The Gladstone Hotel on Wednesday nights so he starts looking for the pencil set that Bill got Sarah. The pencil set has already been found once, by me, five years ago, in a garbage can on the sidewalk near the Art Gallery of Ontario.
It was the year before A. and I moved to Toronto, when we were in the city for a production of one of his plays. It was summer, which meant that all the garbage was extra-stinky. We were one crosswalk away from the gallery when I saw a peripheral flash of baby blue tissue paper. The light changed in our favour and I tried to ignore what I'd seen, taking a few steps into the crosswalk. Then, bested by curiosity, I said "just a sec" and turned back.
I held my breath and scooted over to the garbage can and saw that indeed, atop the heap there was a tissue-lined gift bag in pristine condition and stuffed with who-knows-what goodies.
A. waited by the curb, already pretending he didn't know me in case I was about to do something embarrassing. He is a man of rather British sensibilities, meaning that, paradoxically, while he is fond of arse jokes, he also has a well-developed sense of propriety. He would never root around in the rubbish. Normally, I wouldn't either because I have a sensitive sniffer and an overactive imagination, which leads quickly to dry-retching. I couldn't help myself though. I've always identified with the phrase "Curiosity killed the cat, but satisfaction brought her back."
I looked both ways to see if anyone was watching (or if there was a hidden camera) then cautiously plucked the gift bag out, relieved that it had been resting on a folded up newspaper (though the paper should've been in the recycling). The Hallmark-quality gift bag was chocolate brown and baby blue. I was wearing a brown dress and blue bead necklace so the present and I were hyper-coordinated. Carrying it made me feel like I was the bohemian co-worker of a woman who'd met the man of her dreams at Milestones and was being thrown a bridal shower at which I'd present her with a gift wrapped to suit her personality, not mine (even if the colours matched my outfit).
I also quickly began to feel like I'd stolen the present, even though I'd merely found it, so I made A. stop for a moment so I could, with something resembling both guilt and pride, shove the present into his backpack. It was a tight fit—a substantial mystery.
We then went into the gallery, paid our admission and learned that the backpack had to be checked. I gave A. a worried what-if-there's-a-bomb-in-there? look, which he returned with a now-you-get-that-there-may-be-consequences-to-garbage-picking look. I unzipped the backpack, peeked into the tissue nest and saw a crisp, new sketchbook and fired a smug, what-consequences? look back.
Having stopped at the pub after the gallery, it was late when we got back to our sublet and pried the gift bag out of A.'s backpack. We sat on the bed and I unpacked it while A. watched. As I'd been taught as a kid, I opened the card first. The silver envelope was embossed with a rose—Carlton, not Hallmark. The front of the envelope had the name "Sarah" printed on it in blue ballpoint and had already been gently opened. The card inside was also baby blue and had a tiny sparkly flower on the front. The Carlton inscription said: "A little note to say thank you… and thank you again." The hand-written inscription, which looked, if not shaky, than a little unsure, said:
These are the tools for your future.
In addition to the spiral-bound sketchbook, Bill had got Sarah a thick, hard cover one too. There was also a slim tin of Staedtler drawing pencils and a shiny three-pack of erasers. "You can use all these things, can't you?" I said to A., who sometimes talked about starting to draw again.
"And here's something for me," I said, pulling out a pleather-bound day planner that started, like the school year, in September.
When I pulled the last item out of the bag, our mouths dropped and we both said, "Whoa."
It was an economy-sized box of ultra-thin Trojans.
"What kind of artist is Sarah, exactly?" said A.
John le Carré once said, "'The cat sat on the mat' is not the beginning of a story, but 'the cat sat on the dog's mat' is." That's how it was with the condoms. Now we had a story. But what was it?
Did Sarah throw the present out because Bill was a total creep implying that she was going to need to sleep her way to the top?
Or was he trying to protect her? Was he saying, have a life, not a baby? Or, trust no one?
Who was he to take either stance? He'd signed the card with just "Bill", not "Love, Bill" or "Warm Wishes, Bill" or "Your Drunk Uncle Bill". Was he a mere acquaintance—someone, say, who frequented the café where Sarah worked, appreciated her barista skills (thus the thank you card), and knew she was going off to art school in the fall? A stalker even?
When I told my friend K. about it later, she wrote that perhaps Sarah was having a "career ladder affair" with Bill, who was a "still important Canadian artist" and judge for an art competition. She described Bill as, "inexperienced in love, keen on wild birds, and a fan of the Group of Seven" who was "naively delighted by Sarah's attentions and even imagined they might have a future together (she claimed she was an avid birder), until she dumped him when her piece was rejected."
It was as plausible as any version I could come up with.
Everyone always wants to know what happened to the condoms. We threw them back in the trash instead of handing them out at the end of parties like we might've done if we thought we could trust Bill. I forgot about the day planner and bought another one, but, when rediscovered, it was used as a prop in a short film. The art supplies, however, have travelled back and forth across the country with us.
A. found the pencils again in the third drawer down in his storage unit. Bill was right: tools for the future, just not Sarah's.
THERE, THERE // When They've Left the Building
Is it weird that the memory of my grandmother's dead body is somehow comforting? When I saw her at the nursing home, muscles slack and mouth open, she looked like an empty jail cell with the door ajar. She had escaped, finally. Feelings are weird little monsters from another world...
~ Grateful Granddaughter
Dear Grateful Granddaughter,
I'm so glad that the memory of your grandmother's dead body is comforting to you. I don't think it's weird at all. I think it's weird that we live in such a death-sanitized culture that we might have anxiety around finding comfort in the bodies of the dead.
Of course, it wasn't always like that. People used to have wakes with their dead grandmothers propped up in the middle of the parlour. Katherine Ashenburg writes in her book, The Mourner's Dance, about "merry wakes" in which, in the 1800s, Newfoundland Catholics (following Irish precedents) did things as outrageous as "tying fishing line to various parts of the corpse to make it nod, wave, or rise in its coffin." She mentions that parlours were used so consistently for waking the dead that they were also known as "wake-rooms". At the beginning of the 20th Century when funeral parlours starting offering wake services, home parlours, no longer needed for the dead, were re-named "living rooms".
I myself grew up in a death-repressed household. My brother and I weren't allowed to go into the church for our grandmother's funeral because the service was open casket. We still had to dress up though, and wait in the car, like dogs.
After the funeral when we got home, my mother quietly took down the photo of my grandmother (my dad's mother) that had sat on top of the piano and hid it. Then we pretended Grandma never existed.
Once, I read about the Pygmies and how they appear to be sort of uncomfortable with death:
When a Pygmy dies, other Pygmies pull down his hut on top of him, and move their camp while relatives cry. Then the dead person is never mentioned again.
In other words, my family acted pretty much like the Pygmies.
I didn't get to see the body of the man who I had loved when he died. This was more than eight years ago now. It bothered me endlessly. I asked his sister if she'd seen his body before it was cremated. She said she'd asked at the funeral home and they said something along the lines of, "Certainly, it'll be $200 for the first hour and $50 for every hour after that."
Her response (at least to me, but maybe to the funeral director too) was, "For that I could get a hot, live guy."
It was the first time someone I really loved had died. With the few details I had, I imagined his body in death over and over again. I became so good at creating this image that it was like a chiaroscuro painting in my mind—both dark and holy—but still unreal.
A couple of years later I had to have my beloved kitty put down. She'd been with me for seventeen years, which, at that point, was almost my entire adult life. The vet told me it was the kindest option, and then suggested we have a last twenty-four hours together and come back the next day. I took my kitty home and held her the whole time, even when I had to go to the bathroom. I called her by all her pet-pet names, told her how much I loved her, and how grateful I was that she'd been in my life. And I fed her ice cream, which she loved, but I wouldn't usually let her have because it gave her the worst gas.
She died, wrapped in a towel and in my arms at the vet's office. Under the circumstance, it was the best kind of death she could've had—both for her and for me. I learned that death is actually an action—I watched it happen. Suddenly, she, like Elvis, had left the building.
That expression—that "Elvis has left the building"—was what announcers would say at Elvis' shows when the encore was over so that fans would disperse. I think that's what I wanted when Jesse—that was his name—died. I wanted to know that he really had left the building and it was okay to disperse. In short, I wanted closure. With Fisby—that was my kitty's name—I learned the lesson I knew intellectually all along, but needed to experience for myself—that the body truly is just a container—as ephemeral, really, as a paper ticket to an Elvis show.
GG, you got to see your grandmother's body as a cell she'd sprung from. Of course you find that comforting. Death was her final freedom.
I'm thinking of you as you remember your grandmother. I know you will remember her well.
To submit a question, confession, or concern to There, There all you have to do is visit this link. Go on, spill your lovely guts.
THREE THINGS // Feastable Content
There was an article published by re/code going around a few days ago on "The Death of Snackable Content". Basically, it argues that we now crave content that's more than bite-sized on the Internet. I think it might be an and not an or issue—we want to snack and feast.
I love long form narrative journalism and essays. So, in the feasting spirit, here are three fantastic pieces—both vintage and contemporary—that you can access on the ever-miraculous word wide web right now. You'll be full when you're done. And then you can go back to reading terrifying listicles like this.
1 / "Frank Sinatra Has a Cold" by Gale Talese, Esquire, April 1966
Esquire rightly calls this piece a "pioneering example of what came to be called New Journalism—a work of rigorously faithful fact enlivened with the kind of vivid storytelling that had previously been reserved for fiction." When the writer arrived in Los Angeles to do a story on Sinatra, the Sultan of Swoon was "under the weather, out of sorts and unwilling to be interviewed" so things unfolded differently than the writer had planned.
2 / "The Mixed Up Brothers of Bogatá", New York Times Magazine, July 2015
This is one of those switched at birth stories, told in a NYT way rather than a Jerry Springer way. It's doubly intense because it involves two sets of twins. It's an extraordinary story—and also like any other story in which long lost relatives finally meet and navigate all the weirdness and tenderness involved.
3 / "Mr Lytle: An Essay" by John Jeremiah Sullivan, Paris Review, Fall 2010
I've tried pushing John Jeremiah Sullivan on you before and I'm doing it again. This is the story of when Sullivan lived with Southern writer Andrew Nelson Lytle as his housemate/caretaker/companion. Lytle was well into his nineties at the time and the story is about him both as a renowned writer and a man who had his faults.


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To the special person whose day starts with Yoga. Celebrate his/her birthday with a Yoga cake of 1kg showing his passion for Yoga. Its taste is as good as its classic look with which you simply cannot go wrong. You can order 1kg Yoga Cake online today; and we will deliver it to your home or the home of your loved ones at your chosen time.
Flavour: Please call us for the flavour options.
Note : 1 Day Prior Order


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An international conference of dermatologists participated by 200 doctors from various countries has concluded here on Saturday after holding discussions on dermatological diseases especially found in the tropical region.
The two-day conference organised by Society of Dermatologists, Venereologists and Leprologists of Nepal also held deliberations on various aspects of dermatological diseases seen around the world.
Coordinator of the conference organising committee Dr Sashi Hirachan said the conference held discussions focusing mainly on the causes of dermatological diseases, their diagnosis and their current situation especially in the tropical region.
He said 200 dermatologists and specialists from Nepal, Pakistan, India, Maldives, Switzerland, among various countries, attended the conference which featured the presentation and deliberations on 35 papers.


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Working together to develop a diverse, strong and effective voluntary and community sector in Bolton
|Organisation Description
|Categories of Work
|Where Do They Work?
|Who Do They Work With?
|Astley Bridge Baptist Church
Our church is available for suitab le activ ities as we have two rooms available for hire. We also have a pre-school playgroup, yoga group, and slimming world group. Sunday service 11.00 am
|Astley Bridge Park Bowling Club
A recreational and social club for bowling and other sports and pastimes, played in the spirit of good sportsmanship, fellowship and companionship.
|Social Inclusion, Physical Activity
|Backcare - Bolton Helpline
Telephone and email helpline responding enquiries around backcare to maintain healthy backs. Offering information to prevent or care for back pain.
|Health and Emotional Wellbeing
|Backup North West
Provision of supported housing to homeless 16 - 25 year olds. Anyone within this age range who needs support in keeping a tenancy can apply. We welcome applications from all sectors of the community.
|, , , Health and Emotional Wellbeing
|, Central and Great Lever
|BME, People with mental health issues
|Bangladesh Welfare Association and Community Centre
|, Social Inclusion, Health and Emotional Wellbeing
|, Central and Great Lever
|Bank Street Unitarian Chapel
Faith Group. Main worship 10.45 am Sundays
|Religion, Social Inclusion
|, Central and Great Lever
|Faith communities, Families / single parents, Children (0 -12), LGBTQ+, Older people
|Bank Street Writers
Creative Writing Activities. First Wednesday of each month, 13.45 - 16.00
|, Social Inclusion, Education & The Arts
|, , Central and Great Lever
|Bank Top United Reformed Church
We provide religious services, girls' uniformed organisations (Rainbows, Brownies and Guides), toddler groups, and lace and craft groups.
|Religion, Social Inclusion, Education & The Arts
|Barbodhan Muslim Welfare Society
Established in 1960, we provide social activities for our 2 thousand members. Our members are active in other community/religious groups as well
|Religion, Social Inclusion, Health and Emotional Wellbeing
|Faith communities, BME, Older people, Women, Young people (aged 13-25)
|Be Strong Project
The Be Strong Project is a rehabilitation programme which is set within the heart of the local community and provides activities to ex offenders, drug/alcohol clients and prolific priority offenders. The activities are varied and consist of FREE Personal Development workshops (attendance is compulsory), FREE gym memberships, Thai Boxing, Swimming, bike riding and much more. Be Strong is not for the faint hearted and requires lots of hard work in order to reach the goals which are; become drug free, free from re-offending and become valuable members of society. You can't change your past but you can change your future and we will help you every step of the way.
|Social Inclusion, Housing, Education & The Arts
|Breightmet & Little Lever
|Offenders / ex-offenders, Homeless people, Alcohol & substance misusers
|Beacon Bolton Counselling Service
Provision of counselling to anyone over the age of 16. Volunteers provide hourly counselling sessions Monday - Friday 7.00 pm - 9.00 pm;
|, , Social Inclusion, Health and Emotional Wellbeing
|Central and Great Lever
|Women, Men
|Beest Explorers
To improve the social, physical and spiritual wellbeongof members through participation in a balanced programme of activities.
|, Physical Activity, Education & The Arts
|Young people (aged 13-25)
Building a brighter future for children with special needs
|, Education & The Arts
|Looked after children, People with learning disabilities, Children (0 -12), Disabled people, Young people (aged 13-25)
|Blackrod Cricket Club
|Physical Activity
|Horwich & Blackrod
|Men, Young people (aged 13-25)
|Blair Project
No one person can create success on their own – it takes team work and a helping hand. The Blair Project is championing a new breed of motorsports talent – kids from ordinary families who want to make it to the highest echelons of motor-sports, but lack the money to compete in major championships.
|Children &Young People, Education & The Arts
|Unemployed people, Young people (aged 13-25)
|Bolton & District Hospital Saturday Council
Bolton and District Hospital Saturday offers simple and affordable everyday Health Cash Plans, Dental Plans and Private Medical Insurance from UK Healthcare, our wholly owned subsidiary.
|Health and Emotional Wellbeing
|, , Greater Manchester, , Central and Great Lever
|Bolton & District Multiple Sclerosis Society
Support for MS sufferers in the Borough. Respite care, adaption in the home and equipment (chairs, beds etc.)
|Health and Emotional Wellbeing
|, Halliwell & Crompton
|Bolton & District Speakers' Club
The aim of the club is to provide a friendly, supported and structured environment for people of all abiliites to develop their speech-making skills. The club meets on the second and fourth Mondays of each month.
|, Social Inclusion, Education & The Arts
|, Central and Great Lever
|Bolton Adult Autism Support
Bolton Adult Asperger Support is a voluntary organisation that exists to provide support to and enhance the health and wellbeing of adults with Asperger syndrome and associated conditions and their carers/families.
|Social Inclusion, Health and Emotional Wellbeing
|Breightmet & Little Lever, Halliwell & Crompton
|Families / single parents, Looked after children, People with learning disabilities, Unemployed people
|Bolton Advanced Motorists
we are dedicated to improving driving standards in the Bolton area. we organise advance driving courses in Feb, May, August and November each year. Practical training sessions take place on sunday mornings from chorley street car park Bolton a limited number of midweek sessions are available. To obtain an enrolment form contact Annette Gamble on 0161 797 1084 or email infoboltoniam.org.uk
|Education & The Arts
|All, Men
|Bolton Alternative Transport
Re-cycling bicycles for re-use. Training in bike mechanical skills, repair and maintainence - promotion of cycling and cyling issues. Spare parts and advice.
|Animals & The Environment
|Central and Great Lever
|Refugees and asylum seekers, People with mental health issues, Young people (aged 13-25)
|Bolton Amnesty International
We support victims of human rights violations worldwide, support for example, domestic violence issues, violence towards women, gay, homeophobia issues, the death penalty. We meet the 4th Tuesday in the month, every 2 months but have subcommittees where appropriate. We fundraise regularly throughout the year.
|Social Inclusion
|Central and Great Lever
|Faith communities, Unemployed people, LGBTQ+, BME, Refugees and asylum seekers, Women, Young people (aged 13-25)
|Bolton Arabic School
We meet about once a month to discuss any issues that are raised.
|Children &Young People, Education & The Arts
|Chorley Roads, Rumworth
|Children (0 -12)
|Bolton Armed Forces Centre
They provide advice and information to local and national government agencies on matters affecting the armed forces. To provide information and advice on issues concerning welfare, housing and pentions.
|Social Inclusion
|Central and Great Lever
|Service and ex-service people