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Flagship Brand Ambassador
We're hiring! Join our team by becoming a Brand Ambassador at MiiR Flagship. Enjoy a dynamic workplace designed to be a tangible extension of MiiR, a Seattle-based active lifestyle company with a mission to empower people for a better future. Situated in a LEED platinum certified building located in the heart of Fremont, Seattle, MiiR Flagship is a unique, experiential retail space that attracts a passionate, open minded, service-oriented, customer-centric team committed to sharing the MiiR story through both product sales and service.
As Brand Ambassador, you'll be a champion of representing MiiR, whether serving up MiiR product on the sales floor or a stellar cortado behind the espresso bar. We have an immediate opening for full time (opening and closing shifts) and/or part time team members. MiiR Flagship is open seven days a week from early morning until 7-9 PM, depending on the day and time of year; evening and weekend work may be required. Though not guaranteed, we try our best to set consistent schedules.
Our company culture can be defined by the following values that also inform how and who we hire:
- Be open
- Find a way
- Start with yes
- Enjoy the ride
Your Responsibilities
- Embrace a role that emphasizes the importance of both sales and service
- Provide exceptional service in a fast paced café/bar and retail setting from the moment a potential customer walks in
- Exercise your knowledge of all MiiR Flagship product and service offerings with customers, as appropriate
- Exercise your knowledge of the MiiR story with customers, including MiiR's mission, giving program and core values, as appropriate
- Take and make customer food and beverage orders
- Keep our space clean, tidy and looking sharp
- Participate in the daily service, opening and closing of MiiR Flagship
- Develop relationships with our regular customers and become familiar with their needs
- Engage with customers during their time in our space. We want them feeling great about their visit!
- Show up on time, in appropriate attire, ready to engage and serve authentically and in a way that elevates the MiiR brand
What You Bring
- 1 +/- years of experience working in a customer service/hospitality/retail role, or similar experience
- Demonstrate an eagerness to learn, grow and contribute to the team beyond coffee and/or beer sectors
- Ability to inform and serve customers on all our offerings, including a wide assortment of beer, kombucha and other beverages
- Showcase a positive, professional attitude in a team setting
- Proactive communication - the ability to identify and clearly communicate needs with colleagues, and receive and respond to feedback
- Enthusiasm for teamwork, communication and most of all, hospitality - taking care of people
- Flexibility and willingness to meet changing business needs - be creative!
- Must have Washington State Food Handler Permit and Washington State Class 12 Mixologist Permit upon start date
- Ability to make coffee drinks efficiently is a bonus (we serve Counter Culture coffee on a La Marzocco machine and serve pour-overs)
Essential Physical Job Functions
- Standing for an extended period of time, walking, stooping, kneeling and squatting
- Pushing, pulling, lifting or carrying up to 50 lbs.
- Computer/POS register and bar equipment usage
- Cleaning, tidying, dusting, organizing, restocking
- Reaching, turning, and performing precision work around our bar area
- Receiving, understanding and carrying out oral and written instructions and requesting clarification when needed
We offer competitive hourly wages based on your experience and productivity, and benefits to full-time team members, including health insurance and paid time off. Tips are an additional part of compensation, and team members are eligible to participate in a company-wide bonus pool.
Send your resume and cover letter to email@example.com, or drop off in person at the MiiR Flagship. But first, learn more about our company, our team, and our giving story. We look forward to hearing from you!
Holiday Shipping Deadlines
Make sure your gift arrives in time for Christmas!
U.S. Shipping Only
|Shipping Method
|Order Deadline
|Standard Ground / Priority
|December 14th
|Express Saver
|December 18th
|2 Day
|December 19th
|December 20th
* For international orders, the last day to order is December 14th. Additionally, we cannot guarantee on time delivery based on international customs processes.


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Attacks by South American theft gangs on traveling jewelry salespersons here in the United States continue to decline, thanks to increased efforts of the FBI and intensive lobbying by the industry's security coalition, says a report by the Jewelers' Security Alliance (JSA).
In the first four months of 1999, for example, there were 128 attacks on traveling salespersons. In the same period in 2000, there were 79. During the first four months of this year, there were 60. "While this is still far too much crime, it is less than half the losses of only two ago," says John J. Kennedy, the president of JSA, which formed the industry security coalition of retailers, manufacturers and trade groups. "The decline demonstrates the effectiveness of the FBI's work [in combating the theft gangs]."
In the two years since JSA-led lobbying began, Congress has increased the budget of the FBI, told the FBI to go after jewelry theft gangs in the same way they treat the Mafia, and to significantly increase its opposition to them. As a result, says Kennedy this month, "we have seen much greater enforcement, including task forces in Los Angles and elsewhere [in the United States], and many more investigations, arrests and prosecutions."Follow JCK on Instagram: @jckmagazine
Follow JCK on Twitter: @jckmagazine
Follow JCK on Facebook: @jckmagazine

subset: commoncrawl


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Dandelion Symphony is the brain child of Icelandic Musician and composer Ragga Gisla and was a starting point to the Nordic Council supported Handmade Sound Project. Einkofi Productions originally developed the funding strategy to get the piece to fruition and it was premiered at the critically acclaimed North AtlanticFlux Music Festival in Spring 2017, consisting of an ensemble of seven musician and singers. It was also recorded for BBC Radio 3 programme Late Junction and broadcasted a week after it premiered in Hull.
The Dandelion Symphony consists of 4 movements composed by Ragga Gisla as she records her own performance on dandelions. Each instrument is allowed to keep its unaltered, original characteristics, and the pitch is not changed either. The only effect on the sounds are different reverbs, or recordings of environmental or regional sounds, either indoors or outdoors.


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The first Indian national to get certified by the largest cruise industry trade association speaks about her experience and why travel agents in India who are interested in selling cruise packages need to do this course. Kashish Jiandani, Business Development Manager, NCLH It was the thirst to learn that took …Read More »
Tag Archives: Cruise Lines International Association (CLIA).
The rise of cruising in India
With an increase in desire to cruise, Indians are also looking at it as a venue for events. Peter Kollar, Head of International Training & Development, Cruise Lines International Association (CLIA), outlines the evolving cruise market. Last month we released our CLIA Asia Cruise Trends report for 2017 which outlined …Read More »
Agents need to be cruise experts
Observing that agent knowledge is a key driving factor towards client decision-making, it is only imperative that travel agents in mature markets like India become cruise experts or otherwise get left behind in the race. As cruising continues to grow in India, it's an ideal time to have a look …Read More »


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It's our goal to provide you with a seamless service no matter where you are located. With that in mind we have our facilities located in US and India.
Please find out our locations below, or visit contact us page to see our headquarters and corporate office details.
|Los Angeles13301 Alondra Blvd. Suite B
Santa Fe Springs, CA 90670
Phone: +1-844-294-5383
|Global Delivery CenterA-509, Mondeal Heights, Nr. Wide Angle Cinema,
S G Highway, Ahmedabad – 380015
Gujarat (India)
Phone: +91 7069043269 / 79
Email: email@example.com
|Surat1/386,Ghanshyam Nivas, Bhaya Mohalla,
Nr. Dutch Garden,Nanpura, Surat, Gujarat 395003


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Content Count30
Last visited
Warranty and aftermarket exhaust systems
bestexhaust replied to bestexhaust's topic in Ownership and EnthusiasmPorsche seems to be very protective of its Porsche exhaust aftermarket items. Hence I suspect they bend the truth about warranty being void if they go aftermarket. Fact is (I suspect) the aftermarket exhaust would not be covered under Porsche warranty, and that's it (if catback). I suggested my customer talk to Far Trading in their state to get the low down on Warranties and the power of retailers... The whole point of a warranty is to guarantee the quality of a product and its components. Voiding it as soon as anyone slightly changes it is highly questionable... Nissan tried that back when I had a performance Nissan, and I simply ignored it, as it varied from dealer to dealer. In the United States, they have outlawed warranty "hostage taking" as it is unfair and anticompetitive.
Warranty and aftermarket exhaust systems
bestexhaust posted a topic in Ownership and EnthusiasmI have a lot of customers ask about warranty and fitting aftermarket exhausts. For most car manufacturers, the cat back section is free to change without affecting overall warranty, but obviously, the exhaust system won;t be covered under the car's manufacturer warranty. With Porsche however, I've been seeing claims that the car's warranty will be void if you fit a muffler that is not Porsche manufactured. Surely this is false and substancially illegal for Porsche to make this claim. Does anyone know for sure? Is there any evidence one way or the other? Thanks Mike
bestexhaust started following cargraphic, Warranty and aftermarket exhaust systems, Black Friday Cyber Monday Sale 15% Off and and 4 others
Why buying from Best Exhaust is better than sex
bestexhaust replied to bestexhaust's topic in Best ExhaustDisregard the Muffler bypass option, If you have a PSE exhaust, then replacing the cats is really the only way to make it louder without changing the mufflers... http://www.bestexhaust.com.au/store/fabspeed-porsche-carrera-catbypass-pipe-p-15551.html http://www.bestexhaust.com.au/store/fabspeed-porsche-carrera-sport-pipe-p-12855.html
Why buying from Best Exhaust is better than sex
bestexhaust replied to bestexhaust's topic in Best ExhaustAssuming you mean for the 997.1 Carrera S... http://www.bestexhaust.com.au/store/fabspeed-porsche-carrera-muffler-bypass-pipes-p-16352.html Free Delivery... Yes, just bypass the mufflers... That's the easiest way for more noise... See here: http://www.bestexhaust.com.au/store/images/Porsche_997_Carrera_Muffler_Bypass_Pipes.jpg
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bestexhaust replied to bestexhaust's topic in Best ExhaustBlack Friday sale starts now!!! 15% Off
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bestexhaust posted a topic in Best ExhaustFriday 25th to Monday 28th November. Use discount code BLKCYBER when checkingout to get 15% off, our already shipping inclusive prices. www.bestexhaust.com.au
New for the Porsche 718 Cayman/Boxster
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New 991 GT3 Exhaust from Capristo
bestexhaust posted a topic in Best ExhaustCapristo Porsche 991 GT3 full valve system with headers and sports catsPart Number: 02PO 086 03 001 Exhaust system for the Porsche GT3 (type 991, 2013-2015), 1.4828 Stainless (~T309), muffler with valves, 200cpi sports cats, manifolds, controller CES-3 http://www.bestexhaust.com.au/store/capristo-porsche-full-valve-system-with-headers-sports-cats-p-16131.html
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bestexhaust posted a topic in Best ExhaustCheck this out. Highest quality carbon fibre and stainless, 50% discount for group buy. at least 5 buyers paid by end of July. Respond here and I'll send you an invoice. http://www.bestexhaust.com.au/store/fabspeed-carbon-fiber-wall-porsche-p-15868.html Mike
bestexhaust posted a topic in Best ExhaustJust to let you know that we now sell cargraphic. If the product you want is not listed, let me know and I'll add it. www.bestexhaust.com.au
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Please. No more GOP complaints about President Obama making Constitutional power grabs. RHINOs (Republicans in Name Only) led by Senator Mitch McConnell (R-KY) and Speaker John Boehner (R-OH) just gave the President;
1. Tacit approval to ratify a 12-nation trade agreement that covers 40 percent of the global GDP and was negotiated in secrecy by President Obama without Congressional input.
2. Agreed to allow the President to draft the implementing legislation for the Trans-Pacific Partnership.
3. Agreed to prohibit any amendments to the TPP.
4. Agreed to circumvent the treaty provisions of the Constitution and ratify this pact with a weakened Regular Order and a simple up-or-down vote.
5. Agreed to a Senate rule that for this one piece of implementing legislation cloture is banned and filibuster by any Senator is prohibited.
6. Agreed to allow this and subsequent Presidents to amend the TPP and involve other nations without any Congressional review.
7. Agreed to allow transnational corporations to challenge any U.S. federal, state or local law or regulation as "trade impeding" and allows that challenge to be heard by a tribunal composed of people who are not required to have Senate ratification and are not under the ethical requirements of the federal judiciary.
8. Agreed that there are to be no judicial reviews in the U.S. or elsewhere of these panel decisions.
9. Agreed to either change U.S. laws if required by these panels or pay fines to the plaintiff corporations from the U.S. Treasury.
10. Agreed that President Obama and his successor will have fast track authority for six years, allowing them to negotiate similar pacts with any other nation.
To add insult to Constitutional injury, the President kept secret the text of this agreement from Congress until March 2015, when only Members could view it in a guarded room in the Capitol basement. Members could not make copies; take notes or share with anyone what they had read.
It is simply unimaginable that Senator Majority Leader Lyndon Johnson (D-TX) or House Speaker Sam Rayburn (D-TX) or a succession of Republican leaders would have tolerated such a Presidential trampling of Congress's constitutional authority to regulate foreign trade or insult Congress by asking them to vote to "fast track" a secret global trade pact whose text Members could not share with advisors and constituents until after the legislative wheels were greased.
In sum, the GOP has given President Obama the largest victory of his second term and they did so over the opposition of a super majority of the President's own party.
The weakening of Congressional and Judicial authority by today's GOP leadership and President Obama is historic and it weakens democracy and our entire form of Constitutional government.
When ratification of the Trans-Pacific Partnership comes to a final vote in several months, those who enacted this fast track legislation must be confronted in Washington and in their 2016 Party primaries.
These Wall Street indebted congressional officials are unfit to rule our nation and must be returned to private life.


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Warren, MI | 586-242-4973
Quality work at a reasonable cost
Give me an overview of what I'll find in your shop. What type of products are there? How many of them are there? Tell me what I can expect to find here, and why I should have confidence in buying from you.


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Home > Toys & Games
Product dimensions :- 26x15x8cm
- Material: Polyurethane foam (PU foam), Eco-Friendly. Inedible.- Use: When you feel upset, lonely, angry, bored or sad, squeezing these squishy toys will give vent to your emotions. It will also help the kids develop their observing ability, cognitive ability, spatial intelligence, intrapersonal Intelligence, etc.- Function: hand pillow, stress relief toys, interior decoration, venting emotions, finger toys, wrist rest pad, party/ birthday/ wedding gifts, squishy collections, DIY products, etc.
EAN : 5021813146506RB code : R166343


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The Echo Week exhibit program is developed to give exhibitors and sponsors optimum contact with the leaders in cardiovascular anesthesiology who are interested in echocardiography and cardiac ultrasound. We anticipate over 300 attendees will join us in Atlanta.
SCA appreciates the support of our exhibitors and sponsors and the success you bring to the meeting. We look forward to seeing you in Atlanta!
Download Package and Rate Information
For information on Exhibitor and Sponsorship opportunities, please contact:
Michele Gallas
Sr. Sales Manager
Phone: 847.375.4853
Email: email@example.com
Exhibitor Opportunities
This is your chance to present your products and services to the global leaders in cardiovascular anesthesiologists with an interest in echocardiography and perioperative ultrasound.
Targeted programming draws over 300 specialized decision makers to Echo Week. We also offer dedicated exhibit hours and a prime location for you to meet and network with our attendees.
Our Gold and Silver support packages include advertising opportunities, as well as on-site recognition signage. We also offer Sponsorship Opportunities for items ranging from the meeting app (with a 96% download rate!) to charging stations, key cards and more.
View the rate sheet for more details.
View Floor Plan
Exhibit Schedule
Echo Week exhibit tables are placed in the foyer directly outside the general session room at the Loews Atlanta Hotel. Exhibitors should plan to have their spaces set up during the following hours, however the best time to network with the attendees will be during the coffee and meal breaks:
Non-CME Symposia
Exhibitors can develop and provide 1-hour promotional or educational programs in accordance with their own guidelines. These activities will not compete with CME programs, affording you an opportunity to present your product as a workshop or host a special activity to present branded or non-branded information to attendees.
Features include:
Advertising Opportunities
We anticipate over 300 attendees in Atlanta with an interest in the fundamentals and advanced applications of perioperative echocardiography.
This event is your chance to maximize your presence through the advertising opportunities available to exhibitors. Purchase a room drop or ad in the Program Book to reach as many attendees as possible.
View advertising opportunities
Thank you to our Echo Week Exhibit Sponsors!
Echo Week 2019 Exhibitor Listing:
American Anesthesiology/MEDNAX
CS Medical LLC -- Founded in 2003, Medical LLC is a leader in developing, manufacturing, and marketing medical devices that are designed to provide high-level disinfection of ultrasound TEE probes. Our products are engineered and developed to minimize the potential of patient exposure to improperly reprocessed TEE ultrasound probes. Beyond high-level disinfection of TEE probes, CS Medical believes in managing the quality of care given to delicate TEE probes. TEE Complete Care™ offering provides solutions for the healthcare facility to minimize TEE ultrasound probe damage and reduce the of contaminating high-level disinfected probes prior to use. The products address the following areas: bedside cleaning, electrical leakage testing, drying, transporting and storing the TEE ultrasound probe. Our objective is to manage the care given to each TEE probe thus allowing for a reduction in healthcare associated infections.
GE Healthcare
National Board of Echocardiography, Inc. -- The National Board of Echocardiography, Inc. is a physician, volunteer-led not-for-profit corporation. Established in 1996 to develop and administer examinations in the field of Clinical Echocardiography. A board certification process has been developed to publicly recognize Diplomates of the National Board of Echocardiography.
Philips Healthcare
Siemens Healthcare
Southeast Medical Books
Wolters Kluwer


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Group Cooking Class
Group Cooking Class
Monthly classes are held at a local commercial kitchen. These are pre-planned and thematic and are available for purchase. After you purchase a class, contact me at firstname.lastname@example.com (or you can reach me through the contact page) to let me know which date you are interested in for the class.
Additionally custom-created group classes are available for a slightly higher fee (depending on group size, minimum 8, no more than 14) at the same commercial kitchen. These can also be arranged at a private home within 30 minutes of Amesbury, MA using your own kitchen and most of your own cookware.


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Motoring Myths: Upgrading To A New Car
Posted by admin at 5 April 2018, at 18 : 35 PM
Few moments in life match the excitement of upgrading to a newer car. But many drivers sell themselves short by believing outdated or false statements. If you're currently in the market for a better vehicle, ensuring that you follow the right road to success is vital.
Here are some of the key aspects that will help you achieve the very best outcomes.
Private Sales Produce The Best Results
When upgrading to a newer car, it's almost certain that you'll sell the existing motor, unless it's being handed down to a family member. Most motorists consider private sales to be far better than part-ex routes, which is probably true. However, there are other options out there.
The right option will largely depend on the quality of the vehicle. Experts like Amigo Towing offer cash for junk cars, which can be very useful when yours is in a bad way. If it's no longer capable of satisfying your needs, there's a chance that private buyers will feel the same. Taking this route can remove that problem entirely.
Different options are still available. As long as you factor in the time, cost, and practicality, you should find a winning solution. When you sell your car in the most productive manner, it gives you a far stronger platform for buying.
Buying New Is The Thing To Do
Purchasing a new car is an exciting prospect, and there's no doubt that leaving the forecourt brings supreme joy. For many years, new vehicles were the only ones capable of evoking that confident and comfort reaction. Nowadays, though, there are many other options out there.
The used marketplace has gained immense popularity due to the rising number of great used vehicles available. Appreciation of depreciation will show that this can be a financially sound decision. This is particularly true when the car comes with a full service history. Choosing a dealership over a private sale will provide an extra source of confidence too.
Alternatively, you could even look at leasing a car. While you may not technically own the vehicle, it can be your best chance to drive a better model. Besides, the financial impacts of depreciation should not be ignored.
I Should Change Model
Some drivers think that a change of motor should signal a noticeable change, which is why they switch to a new manufacturer. Others, meanwhile, firmly believe in staying loyal to their favorite model. In truth, there is no one right or wrong answer. The best thing is to do what's right for you.
Upgrading to the newest version of your old and trusted car can make perfect sense. Then again, changing needs may mean that switching to a bigger vehicle or different manufacturer may be necessary. The upgrade may even include moving to hybrid or electric technology. Either way, a test drive and checking the IIHS ratings are key. After all, you need to buy with confidence.
With the necessary research, you should have no problems discovering the very best motor for your ongoing driver needs. If that doesn't give you a reason to smile, what will?


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San Jose Convention Center
150 West San Carlos Street
San Jose, CA 95113
See below for properties offering discounted hotel rates to conference attendees. BOOK EARLY to ensure the best rate & be sure to tell the reservations agent that you're booking with the Watermark Conference for Women.
Marriott San Jose
301 South Market Street | San Jose
$287 per night
CLICK HERE to book
302 S. Market St | San Jose, CA
$299 per night
CALL 855-811-0277 to book
Cut off: 1/31/19
Courtyard Marriott
11 Holger Way | San Jose, CA
$179 per night
CLICK HERE to book
Cut off: 1/31/19
Hilton- block closed
Hyatt Place
282 Almaden Blvd. | San Jose
$229 per night
CLICK HERE to book
Cut off: 1/31/19
We highly encourage the use of public transportation and/ or carpooling to minimize time spent in traffic and looking for parking. If you're not within driving distance, please consider flying into San Jose via our Official Travel Partner, Alaska Airlines.
No matter how you travel, please consider that several thousand attendees will be arriving at the Convention Center at approximately the same time on conference day and plan for the possibility of delays.
The Convention Center Station is located in front of the McEnery Convention Center and is served by VTA Light Rail and VTA Bus Lines 23, 323, and DASH. Other nearby VTA Bus Lines include: 22, 63, 64, 65, 66, 68, 72, 73, 81, 82, 181, and 522.
Pre-purchase your Day Pass (good for unlimited rides all day) for $7 using VTA's EventTIK smartphone app.
You can plan your trip using the trip planner at tripplanner.vta.org or call VTA Customer Service. Visit vta.org for more information.
Caltrain provides rail service between San Jose and San Francisco and is an option for attendees that wish to travel south to attend. The San Jose Diridon station is across the street from SAP Center (so arriving attendees could potentially walk there to take a shuttle to the Conference) or they could take the VTA Light Rail from the Diridon station to the Convention Center (two stops away).
BART (Bay Area Rapid Transit) is another transit system that focuses on transportation through the East Bay and San Francisco. Attendees can easily take BART to Milbrae and transfer to the Caltrain to head to San Jose for the Conference. There are also bus options (VTA) that will allow attendees to get to Downtown San Jose from the Fremont station.
Visit PARK SJ for driving directions and the latest parking maps and pricing for the Convention Center and the rest of downtown San Jose.
Click here or the map below for suggested parking around the Convention Center (PDF)
(click to enlarge)
To book convenient and affordable parking, we recommend using SpotHero, the nation's leading parking reservation app.
To reserve your parking spot today, visit the Watermark Conference for Women SpotHero Parking Page and book a spot with rates up to 50% off drive-up.
New to SpotHero? Download the SpotHero app.
Never miss a registration announcement again—plus keynote updates, podcast reminders, and a monthly newsletter that brings conference day speakers to your inbox all year long!


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What are the Best Debt Consolidation Companies in South Pasadena CA
Who has the best South Pasadena debt consolidation services?
South Pasadena consolidation services offer a way of taking multiple debts and taking them all and wrapping them together into one payment. So if you have 5 different South Pasadena loans, each with a different interest rate and length, a South Pasadena debt consolidation loan will pay these off and then you will be left with one South Pasadena loan with a total on the loan of all the paid off loans. The best part is that these types of South Pasadena consolidation loans typically have lower interest rates than the ones you are getting rid of.
What is the best place in California to find South Pasadena credit consolidation lenders?
Search online for the best debt consolidation lenders in South Pasadena CA. The reason the best place to find South Pasadena debt consolidation lenders in California is because you will be able to find the right South Pasadena lender for you. Your local South Pasadena banks may not specialize in the right South Pasadena consolidation loans for you or have California lending standards that are too stringent. By searching for the best California debt consolidation loans online, you can assure yourself of getting the right South Pasadena loan.
More On Debt Consolidation Loans in South Pasadena CA
As I said above, prior to the credit crunch we had basically "easy money" whereby the South Pasadena banks were encouraged to lend to pretty much anyone in California with a pulse. As the economy tanked and their South Pasadena debts mounted, a large portion of these people who got easy credit are now looking for South Pasadena credit consolidation programs. These South Pasadena loans can be obtained but they are just like any other South Pasadena loan. Your California credit rating, your ability to repay the South Pasadena loan, an availability of collateral to secure the loan and the current South Pasadena bank policies on California lending will determine if you get a South Pasadena consolidation loan or not.
Where Is The Best South Pasadena Loan Consolidation Service?
As I discussed above, the typical South Pasadena loan consolidation service usually has a lower interest rate than the standard California loan that is putting you in the poor house. Be aware that just because you are in trouble with your South Pasadena monthly payments, does not mean that you will automatically get South Pasadena loan consolidation.
Where Can I find The Best South Pasadena Debt Consolidation Help?
I spoke of collateral before and this is a big aspect of why South Pasadena debt consolidation lenders may give you South Pasadena loan consolidation. Unsecured South Pasadena loans are the riskiest loans to give by a California lender. This is because if the South Pasadena loan is defaulted on, the bank cannot get the money back. By requiring collateral to provide security on a loan (as some South Pasadena banks do for South Pasadena debt consolidation loans) the bank reduces their risk for the loan. A secured South Pasadena loan allows the lender to offer the loan to you and then to offer the South Pasadena loan at a reduced interest rate. Be careful to make sure you pay off the South Pasadena credit card consolidation loan on time. If you were required to put up collateral and you default, the South Pasadena bank will take that collateral as a way to recoup their loss.
Where Can I find The Best California Debt Consolidation Help?
Aromas San Marino Somerset Stevinson Fawnskin Dinuba Olivehurst Albion Los Olivos Oak Run El Cajon Baker California City Mount Wilson Red Mountain Nicasio Goleta Orangevale Buena Park Lamont Montclair Calabasas Los Angeles Silverado Palm Desert Crest Park Carmel Valley Rodeo Milpitas Indian Wells Pittsburg Mountain Center Lancaster Quincy Banning Bridgeville New Almaden Baldwin Park Inglewood Manton Palmdale Emigrant Gap Helm Holt Crows Landing Calexico Garden Valley Los Banos Yreka Yucca Valley Buellton Rosamond City Of Industry Hilmar Fairfield Pasadena Bodega Bay Calistoga Beverly Hills Gazelle San Luis Obispo Burbank Colton Arbuckle Piercy Wishon La Palma March Air Reserve Base Greenwood Annapolis Occidental Playa Vista Weldon Vernalis Thermal Union City Vallecito San Gregorio Paramount
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Here is a list of the events we know about where DevSecOps content is being shared. If you have an event that you'd like us to post. Please email us with the info.
Back to All Events
Earlier Event: September 23AppSecUSA 2015
Later Event: October 20Compliance at Velocity in New York


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The five-leg pedestal on wheels is reliable and sturdy no matter how you roll. The rest is pure style. The feel of leather and the easy-to-use tilt mechanism are perfect when you want to lean into your work in comfort.
Our online fabric options are fashionable, cleanable, and durable. A perfect combination.
Follow the link below to order your free fabric samples.
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DWR Concrete Coatings has a wide selection of premium epoxy and polyurea coatings to yield outstanding results every time.Get Free Pricing
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Discover poets and comedians in NYC
Corina Copp is a writer and theater artist based in New York. She is the author of The Green Ray (Ugly Duckling Presse, 2015); and chapbooks All Stock Must Go (Shit Valley Verlag, 2014); Miracle Mare (Trafficker Press, 2013); and Pro Magenta/Be Met (Ugly Duckling Presse, 2011). Her poetry, performance texts, and critical writing can be found in Cabinet, BOMB, Corrected Slogans: Reading and Writing Conceptualism (Triple Canopy, 2013); The Sonnets: Translating & Rewriting Shakespeare (Telephone Books, 2012); The Daily Pen American, SFMOMA's Open Space, The Claudius App, Boston Review, Fence, and Cambridge Literary Review, among other publications. She is developing a three-part play based on the works of Marguerite Duras titled The Whole Tragedy of the Inability to Love. Copp is a former Lower Manhattan Cultural Council Workspace writer-in-residence and curator at The Segue Foundation; and is a 2014 New York Foundation for the Arts Fellow in Poetry. Since 2010, Copp has been a staff writer for Harriet.
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Who doesn't love deviled eggs and there are so many variations on the standard recipe. This is one that I found works really well with our sweet and spicy jalapeños. An explosion in every bite!
12 large eggs
3 tablespoons white sugar
3 tablespoons of cream cheese
1/3 cup mayonnaise
1 teaspoon Sriracha
2 teaspoons Dijon mustard
1 tablespoon white vinegar
salt and black pepper to taste
1/4 cup real Bacon Bits
6 candied jalapeños slices, deseeded and cut in half
My NEW FAVORITE way to cook eggs! In a large pot place a steamer basket and fill until the bottom of the steamer is covered. Place eggs in the steamer and bring water to boil and then turn to a simmer and place a lid on top of the pot for 15 minutes. Place eggs in a pan of ice water and let cool before peeling. Perfect every time!
Slice eggs in half and place yolks in a large bowl. In a smaller bowl mix the first 8 ingredients together with a mixer. Beat well. Taste to adjust to your preference. Fill egg white halves with yolk mixture. Then place the sliced candied jalapeños ends into each egg and sprinkle with Bacon Bits


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After last week's trip to the moon, (read Becky's review here), Doctor Who went all Agatha Christie this week, although naturally there was a non-Earthly twist or two.
'Mummy on the Orient Express' provided yet another state the obvious title, although admittedly quite a charming one. Dressed in a gorgeous 1920s flapper dress which I don't even need to Google far to know caused many a Whovian fan-boy heart to flutter, Clara (Jenna Coleman), joined The Doctor (Peter Capaldi) for what turned out to be their 'last hurrah', despite last week's colossal, and completely called for, row. Anyway, as this was an episode of Doctor Who, and not Downton Abbey, despite what the lavish surroundings would suggest, all is not quite what it seems on board the beautiful space train.
In actual fact, and again you probably got this from the title, there is a Mummy on board, picking off victims one by one, with one particular grandmother murdered even before the opening credits. Phewf. From the moment the victim sees the Mummy, and they alone see it, as, chillingly, it is invisible to everyone else, they have exactly 66 seconds to live. A little specific, The Doctor is quick to establish. Perhaps this is more than just your average Ancient Egyptian monster?
In typical murder mystery fare, it turns out there are plenty of smart alecs aboard who are willing to help, including professors, doctors and ex-soldiers, continuing the running theme of the series. Also on board is Frank Skinner, in a star turn as chief train engineer Perkins. Whilst it wasn't necessarily a bad job from the comedian, far from it, he could happily turn up again and it would totally work, you couldn't help but feel that, unlike other guest turns or even the tiny cameo from the uber cool singer Foxes at the start of the episode, the show was doing more a favour to him as a Whovian than it was to audiences. But ho hum.
Whilst 'Mummy…' was good fun, and certainly one of the more visually memorable situation specific episodes of recent times, it didn't really contribute a whole lot to, well, anything really. As I mentioned earlier, Clara's 'wobble' of last week, is all but ignored as she walks straight into shot just a few minutes in, virtually arm in arm with The Doctor. Her later U-turn on her original feelings, whilst not remotely surprising given her evident reluctance to walk away as the episode went on, did her a disservice as a companion shaping up to be just a little tougher, and more confident in her own mind than previous doe-eyed travel pals have been. She's had quite a few chances to shine in recent episodes, and all credit to Jenna Coleman for some really cracking performances, it just that this wasn't one of her character's better moments.
The monster, and indeed its architect, whilst clever in concept during the middle chunks of the episode, weren't really explained by the end, leaving us wondering, and perhaps hoping, that something more will come of them later. This was another episode lacking in the Missy department, so perhaps they're in some way connected with her?
The monster, and indeed its architect, whilst clever in concept during the middle chunks of the episode, weren't really explained by the end, leaving us wondering, and perhaps hoping, that something more will come of them later. This was another episode lacking in the Missy department, so perhaps they're in some way connected with her?
All that said, the episode did deal well with the elements of this new, slightly reckless Doctor that we're still getting used to just as much as Clara is. In what was probably one of the best scenes of the episode, Clara, having once again lost her faith in this Doctor who blatantly lies to her face and asks her to do the same, awakens to find herself on the beach of a far-off planet, wrapped in a chintzy blanket. (Perhaps it will turn up as a blouse later?) Still not entirely sure what's happened, when she asks about their fellow passengers survival, she's met with exactly the sort of darkly sarcastic reply that only Capaldi can really pull off: 'No, I just saved you. I let everyone else suffocate'. Whilst a brilliant line in itself, this also showed a wonderful awareness of The Doctor's increasingly callous attitude, and isn't a line we'd really have heard from any other recent incarnation of the character.
So, whilst I had a few issues with 'Mummy on the Orient Express', and its brushing under the faux 1920s carpet of several key things, I'm not going to argue too much with an episode that included the line Are you my Mummy?, to a mummy, in what was generally an excellent script from newbie Jamie Mathieson.
Next week's 'Flatline' looks like quite a Clara-centric episode, which might give us some much-needed answers as to her future in the police box and stop me harping on about it too much.
But Becky will be telling you all about that, so keep an eye out for her review!
You can check out Becky's thoughts on Time Heist here.
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You can check out Becky's thoughts on Time Heist here.
Follow @AssortedBuffery on Twitter
Or like our Facebook page


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Several years back I worked for a hair salon in Downtown Seattle. The coffee we served to our clients was called Herkimer and there was only one place nearby that sold it. It was two miles up the road at a coffee shop called "Porchlight".
There were many days when I would clock in for my shift and our store manager Jaimie would tell me that we were out of this precious drink and that I needed to go get more. I'd take the challenge and hop on my bike and bust up the hill. I'd arrive sweaty and out of breath. Zac, the owner, would greet me and I'd mumble something about a bicycle and a hair salon as he handed me the coffee.
During this time, Porchlight was just a seedling in the Capitol Hill neighborhood of Seattle. And now, a few years later, it's a strong tree bearing beautiful fruit to an ever growing neighborhood.
And if I'm honest, there's a part of me that weeps for Capitol Hill and wishes it was still the best kept secret of the city. However, I'm thankful that as it is beginning to burst at its seams, there's a place like porchlight eager to welcome people and help them create a home. These are the things that make a city valuable. Not just pretty buildings and high tech jobs, but people creating good things. People creating home.


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Wet weather projected around 10:30-11am
Is there a shorter route available or are we just going to get very wet?
If you are new, please read our newcomer information.
This is the third of this year's nine Winter Training Series Rides hosted jointly by CBC and the Seattle Randonneurs. WTS starts January 5th and will end on March 2nd. During this nine-ride series, distances progress from about 34 miles to 100 miles. WTS is a great way to do some early season training, with a natural buildup of miles, while enjoying the company of other riders. Winter riding is tough enough--this gives you a chance to do it with friends.
This ride starts and ends at Bellevue's Newport Hills Park and Ride. This year's route is a reversal of last year's new route but keeps us mostly off the perennially busy Issaquah-Hobart Road. We first head south through Renton and out the Cedar River Trail. From there it's up 196th to Covington and Maple Valley to Hobart. Then up Tiger Mountain Road from the south and back via Maple Valley and May Creek Park Roads to Newcastle. Along the way we follow a short stretch of the unpaved section of the Cedar River Trail onto one of our favorite roads: Dorre Don. Unfortunately, one of our favorite coffee stops, Ristretto's Coffee comes a little early in the ride going this direction (26.5 miles), so folks may want to wait until we get to the Hobart Food Market at mile 37 for a brief stop.
Generally, all the Winter Training Series rides start at 9:00 AM except the last one. The 100-miler on February 23rd will start at 8:00 AM. Please be at the start early enough to sign in, pump up your tires, adjust your fenders, and put all your warm clothes on. Sign-in starts at 8:30 AM, ride announcements are at 8:50 AM, and we start promptly at 9:00 AM - no waiting for those still arriving. Though there will usually be a number of ride leaders on the course, these are self-paced and self-guided rides with friendly encouragement and lots of camaraderie. THERE ARE NO REGROUPS IN THIS SERIES. For more general information about this series, please follow the link below.
VERY IMPORTANT: If you want a cue sheet, please go to the RideWithGPS link below and print one out for yourself. There won't be any available from the ride leaders.
All rides are rain or shine. We might shorten a route for really foul weather, but the only outright cancellation will be for snow or ice. We may delay the start if we believe that the conditions will be safer a bit later in the day. CHECK THIS WEBPAGE BEFORE YOU LEAVE HOME.
To save time and to make the sign-in process much easier, please register for this ride. If you already have a Cascade account make sure you are logged in and click the <Register> button at the top of the ride description, then make sure to check the box indicating you have read the Waiver & Release, add your emergency phone number and click <Save Registration> button. Don't worry if you can't make the ride, you don't need to unregister. If you don't have a Cascade account consider setting one up! It only takes a couple of minutes and doesn't cost you anything. If you've pre-registered all you need to do is check off your name at the start of the ride – no lengthy sign-in procedure.
Directions to Start: From I-405 take Exit 9. The parking lot is on the east side of the freeway. THERE ARE NO RESTROOMS AT THE START - (There's a Starbucks at Exit 7 - 2 miles south of P&R.)
Is there a shorter route available or are we just going to get very wet?


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Vectors in gene therapy
Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
All viruses bind to their hosts and introduce their genetic material into the host cell as part of their replication cycle. This genetic material contains basic 'instructions' of how to produce more copies of these viruses, hacking the body's normal production machinery to serve the needs of the virus. The host cell will carry out these instructions and produce additional copies of the virus, leading to more and more cells becoming infected. Some types of viruses insert their genome into the host's cytoplasm, but do not actually enter the cell. Others penetrate the cell membrane disguised as protein molecules and enter the cell.
There are two main types of virus infection: lytic and lysogenic. Shortly after inserting its DNA, viruses of the lytic cycle quickly produce more viruses, burst from the cell and infect more cells. Lysogenic viruses integrate their DNA into the DNA of the host cell and may live in the body for many years before responding to a trigger. The virus reproduces as the cell does and does not inflict bodily harm until it is triggered. The trigger releases the DNA from that of the host and employs it to create new viruses.[citation needed]
The genetic material in retroviruses is in the form of RNA molecules, while the genetic material of their hosts is in the form of DNA. When a retrovirus infects a host cell, it will introduce its RNA together with some enzymes, namely reverse transcriptase and integrase, into the cell. This RNA molecule from the retrovirus must produce a DNA copy from its RNA molecule before it can be integrated into the genetic material of the host cell. The process of producing a DNA copy from an RNA molecule is termed reverse transcription. It is carried out by one of the enzymes carried in the virus, called reverse transcriptase. After this DNA copy is produced and is free in the nucleus of the host cell, it must be incorporated into the genome of the host cell. That is, it must be inserted into the large DNA molecules in the cell (the chromosomes). This process is done by another enzyme carried in the virus called integrase.[citation needed]
Now that the genetic material of the virus has been inserted, it can be said that the host cell has been modified to contain new genes. If this host cell divides later, its descendants will all contain the new genes. Sometimes the genes of the retrovirus do not express their information immediately.[citation needed]
One of the problems of gene therapy using retroviruses is that the integrase enzyme can insert the genetic material of the virus into any arbitrary position in the genome of the host; it randomly inserts the genetic material into a chromosome. If genetic material happens to be inserted in the middle of one of the original genes of the host cell, this gene will be disrupted (insertional mutagenesis). If the gene happens to be one regulating cell division, uncontrolled cell division (i.e., cancer) can occur. This problem has recently begun to be addressed by utilizing zinc finger nucleases[1] or by including certain sequences such as the beta-globin locus control region to direct the site of integration to specific chromosomal sites.
Gene therapy trials using retroviral vectors to treat X-linked severe combined immunodeficiency (X-SCID) represent the most successful application of gene therapy to date. More than twenty patients have been treated in France and Britain, with a high rate of immune system reconstitution observed. Similar trials were restricted or halted in the USA when leukemia was reported in patients treated in the French X-SCID gene therapy trial.[2] To date, four children in the French trial and one in the British trial have developed leukemia as a result of insertional mutagenesis by the retroviral vector. All but one of these children responded well to conventional anti-leukemia treatment. Gene therapy trials to treat SCID due to deficiency of the Adenosine Deaminase (ADA) enzyme (one form of SCID)[3] continue with relative success in the USA, Britain, Ireland, Italy and Japan.[citation needed]
Adenoviruses are viruses that carry their genetic material in the form of double-stranded DNA. They cause respiratory, intestinal, and eye infections in humans (especially the common cold). When these viruses infect a host cell, they introduce their DNA molecule into the host. The genetic material of the adenoviruses is not incorporated (transient) into the host cell's genetic material. The DNA molecule is left free in the nucleus of the host cell, and the instructions in this extra DNA molecule are transcribed just like any other gene. The only difference is that these extra genes are not replicated when the cell is about to undergo cell division so the descendants of that cell will not have the extra gene.[citation needed]
As a result, treatment with the adenovirus will require readministration in a growing cell population although the absence of integration into the host cell's genome should prevent the type of cancer seen in the SCID trials. This vector system has been promoted for treating cancer and indeed the first gene therapy product to be licensed to treat cancer, Gendicine, is an adenovirus. Gendicine, an adenoviral p53-based gene therapy was approved by the Chinese food and drug regulators in 2003 for treatment of head and neck cancer. Advexin, a similar gene therapy approach from Introgen, was turned down by the US Food and Drug Administration (FDA) in 2008.[citation needed]
Concerns about the safety of adenovirus vectors were raised after the 1999 death of Jesse Gelsinger while participating in a gene therapy trial. Since then, work using adenovirus vectors has focused on genetically crippled versions of the virus.[citation needed]
The viral vectors described above have natural host cell populations that they infect most efficiently. Retroviruses have limited natural host cell ranges, and although adenovirus and adeno-associated virus are able to infect a relatively broader range of cells efficiently, some cell types are resistant to infection by these viruses as well. Attachment to and entry into a susceptible cell is mediated by the protein envelope on the surface of a virus. Retroviruses and adeno-associated viruses have a single protein coating their membrane, while adenoviruses are coated with both an envelope protein and fibers that extend away from the surface of the virus. The envelope proteins on each of these viruses bind to cell-surface molecules such as heparin sulfate, which localizes them upon the surface of the potential host, as well as with the specific protein receptor that either induces entry-promoting structural changes in the viral protein, or localizes the virus in endosomes wherein acidification of the lumen induces this refolding of the viral coat. In either case, entry into potential host cells requires a favorable interaction between a protein on the surface of the virus and a protein on the surface of the cell.[citation needed]
For the purposes of gene therapy, one might either want to limit or expand the range of cells susceptible to transduction by a gene therapy vector. To this end, many vectors have been developed in which the endogenous viral envelope proteins have been replaced by either envelope proteins from other viruses, or by chimeric proteins. Such chimera would consist of those parts of the viral protein necessary for incorporation into the virion as well as sequences meant to interact with specific host cell proteins. Viruses in which the envelope proteins have been replaced as described are referred to as pseudotyped viruses. For example, the most popular retroviral vector for use in gene therapy trials has been the lentivirus Simian immunodeficiency virus coated with the envelope proteins, G-protein, from Vesicular stomatitis virus. This vector is referred to as VSV G-pseudotyped lentivirus, and infects an almost universal set of cells. This tropism is characteristic of the VSV G-protein with which this vector is coated. Many attempts have been made to limit the tropism of viral vectors to one or a few host cell populations. This advance would allow for the systemic administration of a relatively small amount of vector. The potential for off-target cell modification would be limited, and many concerns from the medical community would be alleviated. Most attempts to limit tropism have used chimeric envelope proteins bearing antibody fragments. These vectors show great promise for the development of "magic bullet" gene therapies.[citation needed]
Replication-competent vectorsEdit
A replication-competent vector called ONYX-015 is used in replicating tumor cells. It was found that in the absence of the E1B-55Kd viral protein, adenovirus caused very rapid apoptosis of infected, p53(+) cells, and this results in dramatically reduced virus progeny and no subsequent spread. Apoptosis was mainly the result of the ability of EIA to inactivate p300. In p53(-) cells, deletion of E1B 55kd has no consequence in terms of apoptosis, and viral replication is similar to that of wild-type virus, resulting in massive killing of cells.[citation needed]
A replication-defective vector deletes some essential genes. These deleted genes are still necessary in the body so they are replaced with either a helper virus or a DNA molecule.[4]
Cis and trans-acting elementsEdit
Replication-defective vectors always contain a "transfer construct". The transfer construct carries the gene to be transduced or "transgene". The transfer construct also carries the sequences which are necessary for the general functioning of the viral genome: packaging sequence, repeats for replication and, when needed, priming of reverse transcription. These are denominated cis-acting elements, because they need to be on the same piece of DNA as the viral genome and the gene of interest. Trans-acting elements are viral elements, which can be encoded on a different DNA molecule. For example, the viral structural proteins can be expressed from a different genetic element than the viral genome.[4]
Herpes simplex virusEdit
The herpes simplex virus is a human neurotropic virus. This is mostly examined for gene transfer in the nervous system. The wild type HSV-1 virus is able to infect neurons and evade the host immune response, but may still become reactivated and produce a lytic cycle of viral replication. Therefore, it is typical to use mutant strains of HSV-1 that are deficient in their ability to replicate. Though the latent virus is not transcriptionally apparent, it does possess neuron specific promoters that can continue to function normally.[further explanation needed] Antibodies to HSV-1 are common in humans, however complications due to herpes infection are somewhat rare.[5] Caution for rare cases of encephalitis must be taken and this provides some rationale to using HSV-2 as a viral vector as it generally has tropism for neuronal cells innervating the urogenital area of the body and could then spare the host of severe pathology in the brain.[citation needed]
Non-viral methods present certain advantages over viral methods, with simple large scale production and low host immunogenicity being just two. Previously, low levels of transfection and expression of the gene held non-viral methods at a disadvantage; however, recent advances in vector technology have yielded molecules and techniques with transfection efficiencies similar to those of viruses.[6]
Injection of naked DNAEdit
This is the simplest method of non-viral transfection. Clinical trials carried out of intramuscular injection of a naked DNA plasmid have occurred with some success; however, the expression has been very low in comparison to other methods of transfection. In addition to trials with plasmids, there have been trials with naked PCR product, which have had similar or greater success. Cellular uptake of naked DNA is generally inefficient. Research efforts focusing on improving the efficiency of naked DNA uptake have yielded several novel methods, such as electroporation, sonoporation, and the use of a "gene gun", which shoots DNA coated gold particles into the cell using high pressure gas.[7]
Physical methods to enhance deliveryEdit
Electroporation is a method that uses short pulses of high voltage to carry DNA across the cell membrane. This shock is thought to cause temporary formation of pores in the cell membrane, allowing DNA molecules to pass through. Electroporation is generally efficient and works across a broad range of cell types. However, a high rate of cell death following electroporation has limited its use, including clinical applications.
More recently a newer method of electroporation, termed electron-avalanche transfection, has been used in gene therapy experiments. By using a high-voltage plasma discharge, DNA was efficiently delivered following very short (microsecond) pulses. Compared to electroporation, the technique resulted in greatly increased efficiency and less cellular damage.
Gene gunEdit
The use of particle bombardment, or the gene gun, is another physical method of DNA transfection. In this technique, DNA is coated onto gold particles and loaded into a device which generates a force to achieve penetration of the DNA into the cells, leaving the gold behind on a "stopping" disk.
Sonoporation uses ultrasonic frequencies to deliver DNA into cells. The process of acoustic cavitation is thought to disrupt the cell membrane and allow DNA to move into cells.
In a method termed magnetofection, DNA is complexed to magnetic particles, and a magnet is placed underneath the tissue culture dish to bring DNA complexes into contact with a cell monolayer.
Hydrodynamic deliveryEdit
Hydrodynamic delivery involves rapid injection of a high volume of a solution into vasculature (such as into the inferior vena cava, bile duct, or tail vein). The solution contains molecules that are to be inserted into cells, such as DNA plasmids or siRNA, and transfer of these molecules into cells is assisted by the elevated hydrostatic pressure caused by the high volume of injected solution.[8][9][10]
Chemical methods to enhance deliveryEdit
The use of synthetic oligonucleotides in gene therapy is to deactivate the genes involved in the disease process. There are several methods by which this is achieved. One strategy uses antisense specific to the target gene to disrupt the transcription of the faulty gene. Another uses small molecules of RNA called siRNA to signal the cell to cleave specific unique sequences in the mRNA transcript of the faulty gene, disrupting translation of the faulty mRNA, and therefore expression of the gene. A further strategy uses double stranded oligodeoxynucleotides as a decoy for the transcription factors that are required to activate the transcription of the target gene. The transcription factors bind to the decoys instead of the promoter of the faulty gene, which reduces the transcription of the target gene, lowering expression. Additionally, single stranded DNA oligonucleotides have been used to direct a single base change within a mutant gene. The oligonucleotide is designed to anneal with complementarity to the target gene with the exception of a central base, the target base, which serves as the template base for repair. This technique is referred to as oligonucleotide mediated gene repair, targeted gene repair, or targeted nucleotide alteration.
To improve the delivery of the new DNA into the cell, the DNA must be protected from damage and positively charged. Initially, anionic and neutral lipids were used for the construction of lipoplexes for synthetic vectors. However, in spite of the facts that there is little toxicity associated with them, that they are compatible with body fluids and that there was a possibility of adapting them to be tissue specific; they are complicated and time consuming to produce so attention was turned to the cationic versions.
Cationic lipids, due to their positive charge, were first used to condense negatively charged DNA molecules so as to facilitate the encapsulation of DNA into liposomes. Later it was found that the use of cationic lipids significantly enhanced the stability of lipoplexes. Also as a result of their charge, cationic liposomes interact with the cell membrane, endocytosis was widely believed as the major route by which cells uptake lipoplexes. Endosomes are formed as the results of endocytosis, however, if genes can not be released into cytoplasm by breaking the membrane of endosome, they will be sent to lysosomes where all DNA will be destroyed before they could achieve their functions. It was also found that although cationic lipids themselves could condense and encapsulate DNA into liposomes, the transfection efficiency is very low due to the lack of ability in terms of "endosomal escaping". However, when helper lipids (usually electroneutral lipids, such as DOPE) were added to form lipoplexes, much higher transfection efficiency was observed. Later on, it was figured out that certain lipids have the ability to destabilize endosomal membranes so as to facilitate the escape of DNA from endosome, therefore those lipids are called fusogenic lipids. Although cationic liposomes have been widely used as an alternative for gene delivery vectors, a dose dependent toxicity of cationic lipids were also observed which could limit their therapeutic usages.
The most common use of lipoplexes has been in gene transfer into cancer cells, where the supplied genes have activated tumor suppressor control genes in the cell and decrease the activity of oncogenes. Recent studies have shown lipoplexes to be useful in transfecting respiratory epithelial cells.
Polymersomes are synthetic versions of liposomes (vesicles with a lipid bilayer), made of amphiphilic block copolymers. They can encapsulate either hydrophilic or hydrophobic contents and can be used to deliver cargo such as DNA, proteins, or drugs to cells. Advantages of polymersomes over liposomes include greater stability, mechanical strength, blood circulation time, and storage capacity.[11][12][13]
Complexes of polymers with DNA are called polyplexes. Most polyplexes consist of cationic polymers and their fabrication is based on self-assembly by ionic interactions. One important difference between the methods of action of polyplexes and lipoplexes is that polyplexes cannot directly release their DNA load into the cytoplasm. As a result, co-transfection with endosome-lytic agents such as inactivated adenovirus was required to facilitate nanoparticle escape from the endocytic vesicle made during particle uptake. However, a better understanding of the mechanisms by which DNA can escape from endolysosomal pathway, i.e. proton sponge effect,[14] has triggered new polymer synthesis strategies such as incorporation of protonable residues in polymer backbone and has revitalized research on polycation-based systems.[15]
Due to their low toxicity, high loading capacity, and ease of fabrication, polycationic nanocarriers demonstrate great promise compared to their rivals such as viral vectors which show high immunogenicity and potential carcinogenicity, and lipid-based vectors which cause dose dependence toxicity. Polyethyleneimine[16] and chitosan are among the polymeric carriers that have been extensively studies for development of gene delivery therapeutics. Other polycationic carriers such as poly(beta-amino esters)[17] and polyphosphoramidate[18] are being added to the library of potential gene carriers. In addition to the variety of polymers and copolymers, the ease of controlling the size, shape, surface chemistry of these polymeric nano-carriers gives them an edge in targeting capability and taking advantage of enhanced permeability and retention effect.[19]
A dendrimer is a highly branched macromolecule with a spherical shape. The surface of the particle may be functionalized in many ways and many of the properties of the resulting construct are determined by its surface.
In particular it is possible to construct a cationic dendrimer, i.e. one with a positive surface charge. When in the presence of genetic material such as DNA or RNA, charge complimentarity leads to a temporary association of the nucleic acid with the cationic dendrimer. On reaching its destination the dendrimer-nucleic acid complex is then taken into the cell via endocytosis.
In recent years the benchmark for transfection agents has been cationic lipids. Limitations of these competing reagents have been reported to include: the lack of ability to transfect some cell types, the lack of robust active targeting capabilities, incompatibility with animal models, and toxicity. Dendrimers offer robust covalent construction and extreme control over molecule structure, and therefore size. Together these give compelling advantages compared to existing approaches.
Producing dendrimers has historically been a slow and expensive process consisting of numerous slow reactions, an obstacle that severely curtailed their commercial development. The Michigan-based company Dendritic Nanotechnologies discovered a method to produce dendrimers using kinetically driven chemistry, a process that not only reduced cost by a magnitude of three, but also cut reaction time from over a month to several days. These new "Priostar" dendrimers can be specifically constructed to carry a DNA or RNA payload that transfects cells at a high efficiency with little or no toxicity.[citation needed]
Inorganic nanoparticlesEdit
Inorganic nanoparticles, such as gold, silica, iron oxide (ex. magnetofection) and calcium phosphates have been shown to be capable of gene delivery.[20] Some of the benefits of inorganic vectors is in their storage stability, low manufacturing cost and often time, low immunogenicity, and resistance to microbial attack. Nanosized materials less than 100 nm have been shown to efficiently trap the DNA or RNA and allows its escape from the endosome without degradation. Inorganics have also been shown to exhibit improved in vitro transfection for attached cell lines due to their increased density and preferential location on the base of the culture dish. Quantum dots have also been used successfully and permits the coupling of gene therapy with a stable fluorescence marker. Engineered organic nanoparticles are also under development, which could be used for co-delivery of genes and therapeutic agents.[21]
Cell-penetrating peptidesEdit
Cell-penetrating peptides (CPPs), also known as peptide transduction domains (PTDs), are short peptides (< 40 amino acids) that efficiently pass through cell membranes while being covalently or non-covalently bound to various molecules, thus facilitating these molecules' entry into cells. Cell entry occurs primarily by endocytosis but other entry mechanisms also exist. Examples of cargo molecules of CPPs include nucleic acids, liposomes, and drugs of low molecular weight.[22][23]
CPP cargo can be directed into specific cell organelles by incorporating localization sequences into CPP sequences. For example, nuclear localization sequences are commonly used to guide CPP cargo into the nucleus.[24] For guidance into mitochondria, a mitochondrial targeting sequence can be used; this method is used in protofection (a technique that allows for foreign mitochondrial DNA to be inserted into cells' mitochondria).[25][26]
Hybrid methodsEdit
Due to every method of gene transfer having shortcomings, there have been some hybrid methods developed that combine two or more techniques. Virosomes are one example; they combine liposomes with an inactivated HIV or influenza virus. This has been shown to have more efficient gene transfer in respiratory epithelial cells than either viral or liposomal methods alone. Other methods involve mixing other viral vectors with cationic lipids or hybridising viruses.[27]
See alsoEdit
- ^ Durai S, Mani M, Kandavelou K, Wu J, Porteus MH, Chandrasegaran S (2005). "Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells". Nucleic Acids Research. 33 (18): 5978–90. doi:10.1093/nar/gki912. PMC 1270952. PMID 16251401.
- ^ Hacein-Bey-Abina, S (July 2010). "Efficacy of gene therapy for X-linked severe combined immunodeficiency". New England Journal of Medicine. 363: 355–364. PMC 2957288 – via Pub Med.
- ^ "About SCID – Missing Body Defense Systems". Severe Immune Deficiency Site.
- ^ a b "The Process of Gene Therapy." Alternate Heals. 8 may 2006. Alternate Medicine, Web. 23 November 2009.[unreliable medical source?]
- ^ Harwood AJ (1994). Protocols for Gene Analysis. 1st. 31. Totowa, New Jersey: Humana Press. doi:10.1385/0896032582. ISBN 978-0-89603-258-3.
- ^ Murakami T, Sunada Y (December 2011). "Plasmid DNA gene therapy by electroporation: principles and recent advances". Current Gene Therapy. 11 (6): 447–56. PMID 22023474.
- ^ https://www.scribd.com/doc/16368929/Genes-and-DNA-A-Beginners-Guide-to-Genetics-and-Its-Applications[full citation needed]
- ^ Bonamassa B, Hai L, Liu D (April 2011). "Hydrodynamic gene delivery and its applications in pharmaceutical research". Pharmaceutical Research. 28 (4): 694–701. doi:10.1007/s11095-010-0338-9. PMC 3064722. PMID 21191634.
- ^ Suda T, Liu D (December 2007). "Hydrodynamic gene delivery: its principles and applications". Molecular Therapy. 15 (12): 2063–9. doi:10.1038/sj.mt.6300314. PMID 17912237.
- ^ Al-Dosari MS, Knapp JE, Liu D (2005). "Hydrodynamic Delivery". Non-Viral Vectors for Gene Therapy. Advances in Genetics. 54 (Second Edition: Part 2 ed.). pp. 65–82. doi:10.1016/S0065-2660(05)54004-5. ISBN 978-0-12-017654-0.
- ^ Krishnamoorthy B, Karanam V, Chellan VR, Siram K, Natarajan TS, Gregory M (July 2014). "Polymersomes as an effective drug delivery system for glioma--a review". Journal of Drug Targeting. 22 (6): 469–77. doi:10.3109/1061186X.2014.916712. PMID 24830300.
- ^ Chandrawati R, Caruso F (October 2012). "Biomimetic liposome- and polymersome-based multicompartmentalized assemblies". Langmuir. 28 (39): 13798–807. doi:10.1021/la301958v. PMID 22831559.
- ^ Yin H, Kanasty RL, Eltoukhy AA, Vegas AJ, Dorkin JR, Anderson DG (August 2014). "Non-viral vectors for gene-based therapy". Nature Reviews. Genetics. 15 (8): 541–55. doi:10.1038/nrg3763. PMID 25022906.
- ^ Akinc A, Thomas M, Klibanov AM, Langer R (May 2005). "Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis". The Journal of Gene Medicine. 7 (5): 657–63. doi:10.1002/jgm.696. PMID 15543529.
- ^ Tiera MJ, Shi Q, Winnik FM, Fernandes JC (August 2011). "Polycation-based gene therapy: current knowledge and new perspectives". Current Gene Therapy. 11 (4): 288–306. PMID 21453278.
- ^ Nimesh S (May 2012). "Polyethylenimine as a promising vector for targeted siRNA delivery". Current Clinical Pharmacology. 7 (2): 121–30. doi:10.2174/157488412800228857. PMID 22432843.
- ^ Kozielski KL, Tzeng SY, Green JJ (June 2013). "A bioreducible linear poly(β-amino ester) for siRNA delivery". Chemical Communications. 49 (46): 5319–21. doi:10.1039/c3cc40718g. PMC 3894248. PMID 23646347.
- ^ Jiang X, Qu W, Pan D, Ren Y, Williford JM, Cui H, Luijten E, Mao HQ (January 2013). "Plasmid-templated shape control of condensed DNA-block copolymer nanoparticles". Advanced Materials. 25 (2): 227–32. doi:10.1002/adma.201202932. PMC 3918481. PMID 23055399.
- ^ Matsumura Y, Maeda H (December 1986). "A new concept for macromolecular therapeutics in cancer chemotherapy: mechanism of tumoritropic accumulation of proteins and the antitumor agent smancs". Cancer Research. 46 (12 Pt 1): 6387–92. PMID 2946403.
- ^ Wagner DE, Bhaduri SB (February 2012). "Progress and outlook of inorganic nanoparticles for delivery of nucleic acid sequences related to orthopedic pathologies: a review". Tissue Engineering Part B: Reviews. 18 (1): 1–14. doi:10.1089/ten.TEB.2011.0081. PMID 21707439.
- ^ Singh BN, Gupta VK, Chen J, Atanasov AG (December 2017). "Organic Nanoparticle-Based Combinatory Approaches for Gene Therapy". Trends in Biotechnology. 35 (12): 1121–1124. doi:10.1016/j.tibtech.2017.07.010. PMID 28818304.
- ^ Copolovici DM, Langel K, Eriste E, Langel Ü (March 2014). "Cell-penetrating peptides: design, synthesis, and applications". ACS Nano. 8 (3): 1972–94. doi:10.1021/nn4057269. PMID 24559246.
- ^ Palm-Apergi C, Lönn P, Dowdy SF (April 2012). "Do cell-penetrating peptides actually "penetrate" cellular membranes?". Molecular Therapy. 20 (4): 695–7. doi:10.1038/mt.2012.40. PMC 3322330. PMID 22472979.
- ^ Reissmann S (October 2014). "Cell penetration: scope and limitations by the application of cell-penetrating peptides". Journal of Peptide Science. 20 (10): 760–84. doi:10.1002/psc.2672. PMID 25112216.
- ^ Mileshina D, Ibrahim N, Boesch P, Lightowlers RN, Dietrich A, Weber-Lotfi F (August 2011). "Mitochondrial transfection for studying organellar DNA repair, genome maintenance and aging". Mechanisms of Ageing and Development. 132 (8–9): 412–23. doi:10.1016/j.mad.2011.05.002. PMID 21645537.
- ^ Yoon YG, Koob MD, Yoo YH (June 2010). "Re-engineering the mitochondrial genomes in mammalian cells". Anatomy & Cell Biology. 43 (2): 97–109. doi:10.5115/acb.2010.43.2.97. PMC 2998782. PMID 21189990.
- ^ Xiang; et al. (2017). "Effective therapeutic gene transfer via inactivated retroviral vectors: a summary of approaches and current literature review". Journal of Therapeutic Biomolecular Research. 18 (14): 288–311.


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Camping And Survival Gear
ProForce Wind & Waterproof Survival Matches -2 Pack
The ProForce Storm Matches are windproof and waterproof and will burn even when wet! THE very best NATO approved matches available. Designed to light in all extreme types of weather, they can withstand immersion in water and cannot be extinguished by wind. Match head burns for 12 seconds. Approximately 25 matches in each box. Weight: 1.6oz per box
Have one of your own? Comment on this product in the additional info section!.


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Validating Moisture Meter Calibration
Whether it is at the lumber mill or on a building project site, the lumber or the wood being used needs to be measured for the correct moisture content. This is routine in large lumber mills throughout the world but is just as important on the job site. Moisture meters provide an easy, convenient and precise way to measure moisture content in wood. Newer, more modern technology has enabled the industry to even more effectively measure and manage moisture in lumber and wood-based building materials. Wagner Meters carries many moisture meters that are specifically designed to measure wood's moisture content and possibly provide an indication of how temperature and relative humidity (RH) are affecting the wood properties (mainly moisture) at any given moment during wood manufacturing or construction. In many ways, effective moisture content control has never been so accessible.
Moisture meters provide instant results. Wood installers and hobbyists alike can run a Wagner meter along the wood surface and read its percentage of moisture content deep into the wood. Additionally, Wagner meters can be set by the user with the correct density setting for the different wood species they want to measure. Correct moisture content is integral to any successful project built with wood.
When Readings Don't Make Sense
However, the mind boggles at readings produced by malfunctioning moisture meters. All measurement instrumentation must be within calibration and periodically verified as such, and if necessary, recalibrated. Moisture measurement instrumentation for wood is no different. Out-of-calibration moisture meters can cause wacky moisture meter readings. Wagner meters, of course, come factory-calibrated for immediate deployment, but over time they occasionally fall out of sync.
Wagner recognizes that for a wood moisture meter to provide accurate results there must be an immediate remedy. That's why the company has developed its calibration verification block, a go-to way of checking a moisture meter's calibration on-site. Lumber mill personnel, builders, and installers can purchase calibration verification blocks with factory certification to ensure their moisture meter is still within factory specification. Just place the moisture meter on the calibration verification block, and it will verify the calibration. If out of calibration, the meter can be sent back to Wagner for re-calibration. The Wagner calibration verification block is designed to work with: MMC205, MMC210, MMC220, MMI1100, L601-3, L610, L612, L620, and L622. The immediacy of moisture metrics also applies to meter maintenance.
Customers have the option of ordering Wagner's calibration verification block with or without a certificate. A certificate verifies that the block met Wagner specifications prior to shipping. Consider a verification block as a cost-effective investment in maintaining the accuracy of a Wagner wood moisture meter.
As Sales Manager for Wagner Meters, Ron has more than 35 years of experience with instrumentation and measurement systems in different industries. In previous positions, he has served as Regional Sales Manager, Product and Projects Manager, and Sales Manager for manufacturers involved in measurement instrumentation.


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Herman Alphonse Conant
Khai Le
Simi Valley, CA
Herman Alphonse Conant, 80, of Simi Valley, CA passed away peacefully on May 2. He leaves behind his beloved bride of nearly 55 years, Martha Jean (Metoyer) Conant, his two sons, Kevin and Douglas, his daughter-in-law Chloe, and his beloved granddaughter, Mara Jade. Herman is also survived by his sister, Mrs. Valma Veronica (Conant) Metoyer, his brothers Willie and Charles Conant, and many nieces and nephews. He was preceded in death by his parents, Roque Conant and Florence (LaCour) Conant Llorens, his brothers, Joseph and Ralph Conant, and his sisters, Ezora (Conant) Bryant Parker, Rosa Mae (Conant) Roque, and Catherine (Conant) Colson Couty. Herman, a United States Army Veteran, was an award-winning television news cameraman for NBC for more than 30 years and a proud member of the Knights of Columbus Council 5803 and the Father McDonagh Assembly 2557. He will be remembered for his warm smile, his easy laugh, and his kind and generous heart. Services will be held on Thursday, May 17th, beginning at 9:30 a.m. at St. Rose of Lima Catholic Church in Simi Valley, California. Memorial donations may be made to the American Cancer Society or the Herman Conant Scholarship Fund through the Knights of Columbus. Herman will be laid to rest at St. Augustine Catholic Cemetery in Melrose, Natchitoches Parish, Louisiana.
- https://www.legacy.com/obituaries/name/herman-conant-obituary?pid=189022175


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We've had a lot of court cases involving Obama's eligibility and they are generally thrown out. I doubt this will be any different.
But WND says the SSN issue is a lot stronger.
If Barack Obama has an immediate eligibility problem, it is more likely to derive from the Social Security Number he has been using for the last 25 years than from his birth certificate.
Ohio private investigator Susan Daniels has seen to that. On Monday, July 2, she filed suit in Geauga County (Ohio) Common Pleas Court demanding that Jon Husted, Ohio secretary of state, remove Obama's name from the ballot until Obama can prove the validity of his Social Security Number.
According to Daniels, she alleges Obama is using a SSN from Connecticut that was issued when he was 15 years old. She did a brute force test of SSNs with the similar numbers and they were all issued to Connecticut residents.
When she ran 10 sequential numbers before and after Obama's, all returned with the documentation "issued 1977-1979 in CT."
When Daniels ran the numbers immediately flanking Obama's, she came to the firm conviction that Obama's number was issued in March 1977 in Connecticut.
By all accounts, as Daniels thoroughly documents, Obama was then a 15-year-old living in Hawaii. There is no record of him even visiting Connecticut in or near this time frame.


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Matt Nosanchuk
Matt Nosanchuk is an experienced attorney who held multiple senior roles in the Obama Administration from 2009-17. He served at the White House as President Obama's liaison to the American Jewish Community and on the National Security Council staff. Matt also worked at the Department of State in the Secretary's Office of Religion and Global Affairs, the Department of Homeland Security in the Office of General Counsel, and the Department of Justice in the Civil Rights Division.
As an Obama Administration appointee, Matt worked on a wide range of domestic and international issues at the intersection of policy, politics, law, strategic communications, oversight, and public engagement. Highlights of his service include his work at DOJ and DHS to advance LGBT civil rights, including marriage equality, which earned him the Attorney General's Distinguished Service Award and the American Bar Association's inaugural Stonewall Award. Highlights also include his role at the White House and State working to secure support for the Iran nuclear agreement, one of President Obama's signature foreign policy accomplishments.
Previously, Matt worked at the Justice Department during President Bill Clinton's second term, in the Senate as Counsel to Senator Bill Nelson (D-FL), in the House of Representatives as Counsel to Ranking Member John Conyers (D-MI) on the House Judiciary Committee, as a law clerk on the U.S. Court of Appeals for the Seventh Circuit in Chicago, at the ACLU of Illinois as a Skadden Fellow and on numerous political campaigns, including the 2008 Obama campaign. Matt also taught as an Adjunct Professor of Law at Georgetown University Law Center.
Throughout his career, Matt has had extensive media experience, including op-eds published in numerous national news outlets. His work has also appeared in essay collections and scholarly journals. Matt is originally from the Detroit area and attended college and law school at Stanford where he was a Truman Scholar. He has a son, Seth, who is a sophomore at Stanford.


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Yesterday was our neighbors' other son's birthday, so once again I made an Angry Birds cake. She wanted a simpler one this time, so I ditched the FBCT technique for good old-fashioned buttercream decorating.
More Adventures in Cake Decorating!
30 Sep 2014 1 Comment
Food and Biking
30 Sep 2014 Leave a comment
in Aichi-ken, Animals, Castles, Flowers, Hikes, Nagoya, Parks, Restaurants and Cafes
Teddy and I haven't been up to too much exciting recently, other than eating, hanging out, and biking.
Adventures in Cake Decorating!
20 Sep 2014 2 Comments
in Aichi-ken, Apartment, Friends, Nagoya, Nishi-ku, Special Events
About a month ago, our new neighbor Angela asked me if I knew of a bakery that made character-themed cakes for children's birthdays. I told her that unfortunately, I did not. About a week after that, she asked if I could make her son an Angry Birds cake for his birthday. I love anything arts and crafts related, so naturally I accepted the challenge! She really liked the looks of the fondant cakes, but I have very limited experience in fondant, and one of the cakes she liked had actually been created by another technique called Frozen Buttercream Transfer. After doing my research on the technique, I moved to the design phase!
Ichinomiya and Domannaka Matsuri
13 Sep 2014 Leave a comment
in Aichi-ken, Animals, Apartment, Festivals, Friends, Ichinomiya, Nagoya, Restaurants and Cafes, Shrines and Temples, Special Events
We've been busy biking around, hanging out, and having fun the past few weekends.


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Incorporating Video into Your Social Media Strategy
Not sure if you want to include video in your social media strategy? Here's a few things to consider. Video produces a 157% increase in organic traffic through search engines and marketers who use video generate revenue 49% faster than those who don't.
Video gets results, and on social media, it can be even more effective. The question at this point isn't IF you should incorporate video into your social media strategy, but how you should be doing it. If you're new to using video, or just looking for a few new ideas, here are 3 ways to successfully optimize video in your social media campaign.
Go Live
Live video is exactly what is sounds like -live video that's broadcasted on social networks as it's happening. Facebook Live is the most recognized live platform, but other social networks are catching on.
If you're new to video, then the idea of going live might leave you with a bit of stage fright. It's understandable but listen to the reasons why live content is so popular right now.
As more business are tapping into the power of video, audiences have started to grow tired of seeing the same thing repeatedly. They're looking for something that's new, refreshing and authentic. Is there anything more authentic than putting yourself in front of a live audience?
Not really.
Audiences find live content more engaging and transparent. Plus, live content taps into their fear of missing out, which only generates more viewership for your videos.
You've heard it repeatedly. Content is king.
In response, you've invested some serious time and energy into creating great content, some of it in the form of blogs. This is good, but no matter how great your blogs are, video is almost always better.
Brands can tap into the best of both worlds by combining the elements of written content with video and then posting them on social media the same way you would a blog.
A problem with blogging is that readers don't always stick through to the end, or they skim over parts. Sometimes this means they're missing out on important information or your marketing message. On the other hand, 55% of people who start watching a video stick through to the end. This is higher than any other type of content.
Video Enhanced Cover Images
Not that long ago, Facebook gave its users that ability to use video as a cover photo. For the average individual user this might not be such a big deal, but for brands on Facebook, this development was huge.
This feature, which allows for a continually looping video of up to 90 seconds, is a great way to showcase your brand to users who are just landing on your page. Cover photo video is engaging and requires minimal commitment on the part of the user.
How are you planning on optimizing video in your social media strategy? At BeeSmart Social Media, we'd love to help you reach your full potential. Contact our social media experts today and let's start talking about your future.