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Term Paper on Fuel Subsidy
Subsidy Term Paper:
Fuel subsidy is the governmental support of the fuel industry and donations into it with the purpose to enable industrial objects pay less for fuel resources and concentrate their expenditures on the production of the required goods and services. Frankly speaking it is not reasonable to carry out fuel subsidy, because the price for oil and gas are extremely high in the modern world and the prices will be only getting higher and higher. Fuel subsidy is supposed to maintain the development of the middle and great business which supports the economics of the country and this intention is understandable. For example, in order to exist, a company has to possess finance for self development, finance for paying salaries, taxes and energy.
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Fuel is one of the most expensive expenditures, so it is obvious that without subsidies many companies would not be able to cope with the taxation burden. Unfortunately, on practice fuel subsidy is carried out poorly. The businessmen who get such subsidies stop to appreciate fuel and energy resources wasting them often without any purpose. A company can purchase much of cheap fuel and burn it down in enormous quantities which affects the economics and the environment badly. In order to stop the waste and teach businessmen appreciate resources fuel subsidies have been reduced considerably in many countries of the world. Evidently, it has provoked disappointment and strikes but it is the only way out to economize natural resources and reduce financial expenditures.
Fuel subsidy is a positive idea but its purpose has been misunderstood, especially in the modern world. Students who are required to prepare good term papers on fuel subsidy will need to explain the purpose of these subsidies, the methods with the help of which the policy is carried out and what spheres are maintained due to the subsidies. Furthermore, the paper should present a well-analyzed list of the advantages and disadvantages of fuel subsidy and every point should be supported with the reliable evidence from the real cases connected with business, finance and environmental protection. The term paper should be interesting and convincing, as one should support his point of view with the true to life facts.
The process of term paper writing has always been difficult for young people, because they have to spend much time on research and analysis of complicated topics. Besides, very few students know how to organize the papers professionally and they need good free term paper on fuel subsidy to improve their knowledge. Writing a term paper requires special skills and knowledge about the standard structure and format of the paper, way of analysis and presentation of data, so a free sample term paper on fuel subsidy in Nigeria completed by the professional writer will raise student's chances to prepare a successful paper himself.
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This is the privacy policy for FitConnect UK LTD (trading as FitConnect), a company registered in England (company number 10254674) with a registered office at 1-3 St Johns Court, Whiteladies Road, Clifton, Bristol, UK. BS8 2QY ("we", "us", "our") in relation to the use of personal information provided in relation to the use of the website and online account and personal finance management services provided at www.fitconnect.io ("the Service").
We know that you'll want to know exactly what information we gather about you as a user of our Service ("User"), and how we intend to use (and just as importantly, not use) that information.
We'll try to keep everything in this document as straightforward as possible, but if there's anything you don't understand or you're not happy about, or if you just want to suggest improvements, please get in touch with us by:
You do not have to supply any information simply to visit the site.
To register to use our service you will have to supply us with personal information. "Personal Information" means information that identifies you. The personal information we collect to register you is your e-mail address.
In order to complete your account, you will also need to provide your date of birth, gender, height, weight and choose a password. Once you have aggregated accounts with us (see "Other people who have to be able to access your information" below for an explanation) , we will have access to that account information.
We keep track of the internet addresses from which Users visit us, and track the page(s) on our site that you visit. We analyse this data for trends and statistics. Please see "Cookies and Other Tracking Technologies".
We will use the personal information which we collect for the purposes of registering you as a User of our services, correspondence, or participating in online surveys to help us enhance our services and products for you.
We also ask for personal information at the point at which you add a fitness account to enable us to ask security related questions if you later ask us to reset your password.
In addition, through a computer system-run process, we will use your personal information and information from your account aggregation for the purposes of analysing it and making suggestions which may enable you to achieve your fitness goal or consider alternative or additional products to the ones which you are using.
These suggestions can be delivered either in the service, by way of email, text messages or via the gym that you are a member of, who will always see this information (see "Other people who have to be able to access your information" below for an explanation). This may involve us sending limited details about you (which will not identify you) to our partners so that we can tailor suggested products for you more accurately.
Any information we collect is used to improve the content of the site, enhance Users' experiences when visiting our site, and customise the content and/or layout of our pages.
We also use personal information in performing statistical analysis of Users' behaviour as a whole; and to help in the creation of anonymised statistical data which we may use at our discretion (including licensing to third parties).
We do not share personal information for any reason that is not disclosed in this Policy or apparent to you at the time the information is requested and we do not sell or rent any personal information.
The Service is licenced by gyms for the purposing of providing the software to their members. If you are a member of a gym that is using the Service, you agree to give the gym permission to see your personal information, account aggregation information and the insights we create.
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We may have to disclose your personal information, if required by law to comply with a judicial proceeding, court order, or similar legal requirement.
If you want to terminate your agreement, email firstname.lastname@example.com using the email address you used when registering for the service. Your account will be closed and your ability to log in deactivated within 30 days.
To access and change your personal information, you should log in to your online account on our website and make the necessary changes.
We will retain your information for as long as your account is active or as needed to provide you services. If you wish to cancel your account or request that we no longer use your information to provide you services contact us at email@example.com. We will retain and use your information as necessary to comply with our legal obligations, resolve disputes, and enforce our agreements.
Once deleted by you the data will be promptly deleted from our systems and we will subsequently be unable to access it.
We reserve the right, however to continue to use any anonymised statistical data which we created during your use of the Service.
You can use the service to provide you with email and text alerts to provide you with useful information, e.g. you have completed a fitness goal. You can disable these alerts at any time by removing these alerts from within your Profile.
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We post customer testimonials/comments/reviews on our website which may contain personal information. We obtain the customer's consent via email prior to posting the testimonial to post their name along with their testimonial. If you wish to update or delete your testimonial, you can contact us at email@example.com.
Our website offers publicly accessible blogs or community forums. You should be aware that any information you provide in these areas may be read, collected, and used by others who access them. To request removal of your personal information from our blog or community forum, contact us at firstname.lastname@example.com. In some cases, we may not be able to remove your personal information, in which case we will let you know if we are unable to do so and why.
Our blog is managed by a third party application that may require you to register to post a comment. We do not have access or control of the information posted to the blog. You will need to contact or login into the third party application if you want the personal information that was posted to the comments section removed.
To see our full security policy please contact us on the details at the top of the privacy policy.
The security of your personal information is important to us. We follow generally accepted industry standards to protect the personal information submitted to us, both during transmission and once we receive it. No method of transmission over the Internet, or method of electronic storage, is 100% secure, however. Therefore, we cannot guarantee its absolute security.
Our website includes Social Media Features, such as the Facebook Like button and Widgets, such as the "Share this" button or interactive mini-programs that run on our site. These Features may collect your IP address or which page you are visiting on our site, and may set a cookie to enable the Feature to function properly. Social Media Features and Widgets are either hosted by a third party or hosted directly on our Site. Your interactions with these Features are governed by the privacy policy of the company providing it.
We may need to modify this privacy policy at any time, so please review it frequently. If we make material changes to this policy, prior to the change becoming effective, we will tell you by email, or by a notice on our homepage so that you are aware of what information we collect, how we use it, and under what circumstances, if any, we disclose it.
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In order to access our website's full features all cookies must be enabled. For more information on how to manage cookies please refer to our "How to manage cookies" section below.
Our use of cookies provides us with general (non-personal) information about how visitors use this website, i.e. the pages which are viewed most often or the most common traffic sources to our website. Cookies are also used across this website to remember customisable user selections to provide you with a more personalised experience.
As is true of most websites, we gather certain information automatically and store it in log files. This information includes internet protocol (IP) addresses, browser type, internet service provider (ISP), referring/exit pages, operating system, date/time stamp, and clickstream data.
We use this information, which does not identify individual users, to analyse trends, to administer the site, to track users' movements around the site and to gather demographic information about our user base as a whole.
We may link this automatically-collected data to personal information in order to allow analysis of the effectiveness of marketing campaigns How to manage cookies.
Your internet browser's security settings or preferences enable you to take control of the information you share online. These can usually be found within your browser's menu tab. You will be presented with the option to accept all cookies, receive an alert when a cookie has been set, or to disable your browser from receiving cookies.
Internet browsers provide the above features in their own distinct ways, so please refer to the help section – located on your browser's menu – for a specific set of instructions on how to change your cookie preferences. For those viewing this website on a mobile device, please refer to your handset guide or technical manual.
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The use of cookies by our partners is not covered by our privacy policy. We do not have access to or control over these cookies.
Our third party partners employ clear gifs (also known as Web Beacons/Web Bugs), images, and scripts that help them better manage content on our site. We may tie the information gathered to our Users' personal information in order to analyse the effectiveness of marketing campaigns.
Our website also contains cookies set by third party websites (i.e. Social networking sites such as Twitter), which provide information and/or services on the FitConnect website. In order to manage these cookies you will have to visit the websites of our third parties and adjust your settings accordingly.
More information on how to control or manage cookies can be found at http://aboutcookies.org/.
Last updated: October 2017

subset: commoncrawl


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Day 844
We are spending the night at the Florida Department of Transportation weigh station located at mile marker 13, west bound Interstate 4, in Seffner, Florida.
This is one of Florida's newly renovated stations, with state of the art scales and utilizes laser technology. When a truck pulls in, it is scanned by 9 laser cameras that presents a 3 dimensional image on the controller's computer screen.
It gives the operator the height, width, length of the tractor and the trailer, as well as distance between axles. Along with the scale, that measures weight of the cab and separate weight of the trailer, it is like a cat scan of the truck.
In talking with the station master, we learned quite a bit about truck weight and violations. For example, some trucks will be randomly selected to be fully inspected, including undercarriage.
As the truck pulls onto the scale, it's weight is compared to it's dimensions and axles,
which determines if the truck is in compliance with the law.
This truck is overweight on one axle. However, the driver can move that axle to distribute the weight more evenly, and therefore come into compliance.
This truck has a length violation. To correct that, the driver can move the king-pin to make the length shorter.
Tidbit of Information: Maximum truck length is 51 feet measured from the front of the cab to the center of the rear axle. By moving the kingpin forward, that moves the trailer closer to the cab, and therefore shortens that distance. Maximum height of tractor trailer is 13 feet 6 inches. The Sphinx is 13 feet 5 inches.
We met 2 other rigs from our Alaska trip here, and will all be going together tomorrow to the Tampa RV Show.


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Bridget Snaith is working on a project looking at how to help Croydon's residents access all the potential health & wellbeing benefits offered by parks. At the same time, the project will explore how new revenue streams and voluntary sector activity can support sustainable park maintenance in the context of population growth, and diminishing public sector resources in the Borough. It will focus on Park Hill, Ashburton, Lloyd Park, Norbury, South Norwood Lake and Happy Valley
You can email your views to Bridget before any design work starts (programmed for end June continuing into July 2017). Or attend an engagement event at Ashburton Park (11am – 3pm 25th June), Park Hill (11am-3pm 2nd July) or Lloyd Park (9th July).
Click here for more information from ASPRA


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The Miami Herald reports Sybrina Fulton will be entering the race to join the 13-member board of Miami-Dade County commissioners.
Fulton said in a Saturday statement that she would continue working to end gun violence. She will challenge Miami Gardens Mayor Oliver Gilbert for the seat that is up for grabs in 2020 because of term limits.
Trayvon's parents head a foundation and have been recognized for their work on gun reform and social justice.
Trayvon was unarmed when shot dead by neighborhood watch volunteer George Zimmerman, who was later acquitted on the grounds of self-defense. His death sparked the Black Lives Matter movement.
Information from: The Miami Herald, http://www.herald.com
Copyright 2019 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


text: In addition, shop bought whitening trays are made to in shape Every person's teeth, not your teeth especially. Because of this they could become loose, and the answer can leak, resulting in gum irritation. Dr. Buccellato will then examine your teeth and gums making sure that they are healthy more http://blingee.com/profile/Joshua260n9ek8


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Project 52 : January | Creative Shoot
P52 | Get in the Frame Project
I will be working on a project for 2018 called "Get in the Frame" to not only challenge myself to get in more images but to also serve as a creative outlet. Things may get weird. #sorrynotsorry
Being a photographer is an absolutely amazing job, but there are times when you tend to feel less creative + projects like these help keep your artistic soul happy. So... I will be posting a journal entry of images for the month + at the end of the year, I will put them all together in a slideshow. I am really looking forward to how this evolves over the year! I hope you do too!


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Sunney I. Chan (陳長謙)
The mechanisms of proton pumping in quinol oxidases
Sunney I. Chan, Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529 and Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, CA 91125, USA
The principles of redox linkage in electron-driven proton pumps will be reviewed and applied to "proton pumping" in the quinol exidases. It is shown how the coupling of electron-gating and proton-gating with redox-linkage is essentialto the operations of the proton pump, including the kinetic competence and the vectional requirement of the process. The proton-pumping in two quinol exidases, mitochondrial cytochrome bc1 and E. coli cytochrome bo3, will be discussed withinthe framework of these concepts.
Tzu-Ping Ko,1 Wen-Yen Ku,1 Meng-Jiun Sui,1 Yu-Wen Liu,1,2 Kin-Fu Chak2 and
Hanna S. Yuan.1
1Institute of Molecular Biology, Academia Sinica, and
2Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan.
Colicins are bacteria toxins secreted by Escherichia coli to kill other Escherichia coli. The cytotoxic activity of a colicin in a colicin-producing cell can be counteracted by binding of the colicin to a highly specific immunity protein. This biological event provides a good model system for the investigation of protein recognition. We have been focusing on the structural study of a DNase-type colicin, colicin E7 (ColE7), which contains a DNase domain that it cleaves DNA in target cells leading to the cell death. We previously determined the crystal structures of the immunity protein Im7 and the one-to-one complex between the DNase domain of ColE7 and Im7 at 1.8 Å and 2.3 Å resolution, respectively. The complex structure explains why the two proteins interact with each other with such a high specificity and affinity.
The structure of the DNase domain of ColE7 contains a HNH motif within which a zinc ion is identified and the zinc ion is bound to three histidine residues and one water molecule in a distorted tetrahedron geometry. The characterization of the metal-dependent DNase activity of ColE7 shows that a divalent metal ion is required and the enzyme is most active with the presence of Mg2+ or Ni2+ as a cofactor. The crystal structure of the phosphate-bound complex determined at 2.0 Å resolution further shows that a phosphate ion is bound to the zinc site. This result suggests that the metal ion in ColE7 is involved in DNA-binding and it is likely that the endonuclease active site is located around the zinc-binding site. However, this putative DNase active site is not directly blocked by the immunity protein. We suggest that the immunity protein blocks the DNA-binding site or induces conformational change in the colicin so that a colicin cannot bind to DNA for hydrolysis.
NMR Studies on Newt (Notophthalmus viridescens) Acidic Fibroblast Growth Factor: Structure, Dynamics, and Interactions.
Chin Yu , Chemistry Department, National Tsing Hua University, Taiwan
Newt acidic fibroblast growth factor (nFGF-1) plays an important role in several cellular processes including cell growth, cell differentiation, angiogenesis, wound healing and other chemotactic activities. Recently, nFGF-1 was cloned and expressed in E. Coli. and the double labeld (15N and 13C) protein (nFGF-1) was prepared. A series triple resonance experiments were carried out. The NMR 3D structured of nFGF-1 were calculated by using distance-geometry calculation followed by the dynamical simulated annealing techniques. The backbone dynamics studies on nFGF-1 were accomplished by using 15N relaxation and heteronuclear NOE measurements and data were analyzed by model-free approach. Glycosoaminoglycans such as heparin and heparan sulfate are known to activate nFGF-1 by inducing the dimerization of the receptor tyrosine kinase and thereby triggering the signal transduction cascade. Molecular level understanding of the binding of glycosoaminoglycans and its analogs to nFGF-1 is necessary for the design of drugs to inhibit nFGF-1 action. A detailed NMR study using an uniformly 15N labeled sample has been carried out for newt nFGF-1 bound to sucrose octasulfate. From the chemical shift perturbation and intermolecular NOEs it was found that those residues belonging to the putative heparin binding domain spanning residues 126 to 142 are involved in the binding of sucrose octasulfate to newt nFGF-1.
Homo- and Heteronuclear NMR Studies of the Purine-rich and Pyrimidine-rich DNA Mini-Loop Hairpins
National Chung-Hsing University, Taichung, 40227, Taiwan
Nucleic acids are special biomolecules; they can serve as a catalyst, aptamer, or a carrier of heritable information. Due to the advancement of powerful techniques such as PCR (polymerase chain reaction) and SELEX (selected enrichment of ligand by exponential multiplication), nucleic acid sequences possessing any required function can now be screened out without much difficulty. Many novel functions for nucleic acids have therefore been discovered, including those involved in enzymatic reactions and monoclonal aptamer etc.
It is obvious that a linear single-stranded nucleic acid sequence can only execute its function when folded into a specific tertiary structure through hairpin formation. Besides, hairpins can also be existent when intra-strand pairing occurs between inverted repeat sequences in double-stranded DNA. It is therefore interesting to study such fundamental motifs.
While tetraloops of the type GNRA and UNCG are prevalent in the RNA structure, triloops are instead the major forms in the DNA structure. In the past, we have been studying purine-rich and pyrimidine-rich triloop hairpins and found they adopt dramatically different structures. The homo- and heteronuclear NMR studies of such compact stable motifs will be discussed, as well as their biological significance.
Metal Binding in Proteins:
The Effect of the Dielectric Medium, Ligand Type and Geometry
Todor Dudev and Carmay Lim*
Institute of Biomedical Sciences, Academia Sinica,
Taipei 11529, Taiwan, Republic of China.
This work attempts to addresses three aspects of metallo-protein chemistry: First, why metals ions tend to bind to proteins directly (in an "inner-sphere" mode) at centers of high hydrophobic contrast? Second, what is the most thermodynamically preferable set of inner-sphere ligands for a given metal ion (e.g. magnesium) in proteins? Third, what is the most thermodynamically preferable coordination geometry of a given metal ion (e.g. zinc) in proteins? To address these questions, a combination of ab initio and continuum dielectric calculations as well as Protein Data Bank (PDB) surveys of metal-binding sites in proteins were carried out. The results show that a low dielectric medium favors the inner-sphere binding of protein ligands to the metal. Using magnesium as an example, the results delineate its most thermodynamically preferable inner-sphere set of inner-sphere ligands and suggest an explanation for the unique role of Mg2+ as a carrier of water molecules that mediate enzymatic hydrolysis reactions. Finally, using zinc as an example, the results show that zinc prefers to be tetracoordinated in a low-dielectric protein environment, hence the tetrahedral zinc binding sites are not necessarily energized states, as proposed in earlier works.
Structure and function of a moonlighting protein: phosphoglucose isomerase/ autocrine motility factor/neuroleukin/differentation and maturation mediator
Chwan-Deng Hsiao1 , Yuh-Ju Sun1, Chia-Cheng Chou1, Wei-Shone Chen2, Rong-Tsun Wu3, and Menghsiao Meng4 1Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan. 2Veterans General Hospital, Taipei, Taiwan. 3Graduate Institute of Biopharmaceutial Science, National Yang-Ming University, Taipei, Taiwan. 4Graduate Institute of Agricultural Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Phosphoglucose isomerase (PGI) plays a central role in both the glycolysis and the gluconeogenesis pathways. PGI has been found to serve functions equivalent to those of autocrine motility factor (AMF), a tumor-secreted cytokine which stimulates cell migration in vitro and metastasis in vivo, and to those of neuroleukin (NLK), a neurotrophic factor for spinal and sensory neurons. Here we present the first complete crystal structure of phosphoglucose isomerase at 2.3 _ resolution from Bacillus stearothermophilus. We also demonstrate that PGI performs cell motility-stimulating activity on mouse colon cancer cells similar to that of endogenous AMF, and that PGI enhances neurite outgrowth in terms of length and density on neuronal progenitor cells similar to that observed for NLK. The results confirm that PGI is NLK and AMF. The structure of PGI is a open twist a/b structure motif consisting of two globular domains and two protruding parts. Based on this substrate-free structure, together with the biological, biochemical and modeling results, we postulate the possible substrate binding-site located within the domains' interface for PGI/AMF based on previous observations that both proteins share the same inhibitor site. The structure further provides evidence to suggest that the top part of the large domain and the protruding loop of PGI/NLK play roles in inducing neurotrophic activity.
DNA chips and Their Applications in Genomic Biotechnology
Konan Peck, Yuh-Pyng Sher, Ji-Yen Cheng and Yun-Shien Lee
Institute of Biomedical Sciences, Academia Sinica
Taipei, Taiwan 115, ROC
DNA microarrays or DNA chips are the most recent technology breakthroughs in genomic studies. The methods allow genome-wide analysis of thousands of genes at a time. Two high capacity systems based on oligonucleotide and cDNA microarray approaches and colorimetric detection method (Microarray/CD) are developed. The oligonucleotide microarray system was designed for typing single nucleotide polymorphism and genetic variations. The cDNA microarray system was designed to simultaneously monitor the expression of thousands of genes in human cells treated with and without external stimuli.
For the oligonucleotide microarray fabrication, a high-capacity multiplex oligonucleotide synthesizer was constructed to simultaneously synthesize 384 discrete oligonucleotides in about 10 hours. The synthesis was based on phosphoramidite chemistry with step yield routinely over 98.5%. The oligonucleotide probes were spotted in microarray format on Nylon filter membranes by an arraying machine developed in-house. The chemistry for immobilizing the oligonucleotide probes on Nylon filter membranes and the protocol for genotyping single nucleotide polymorphisms in a gene have been developed.
For the cDNA microarray system, the target genes were selected based on an in-house constructed UniClone database containing the longest EST clone for every gene cluster built by the NCBI Unigene clustering. Based on the UniClone compilation, an indexed cDNA clone set containing thousands of non-redundant gene clones has been constructed for genome-wide gene expression studies. To array thousands of non-redundant cDNA fragments on Nylon filter membranes, a high capacity arraying machine capable of holding 31,000 of PCR products at a time and depositing 31,000 spots on a piece of 1.8 cm by 2.7 cm Nylon membrane have been developed. Several on-going applications of the cDNA microarray system implemented in our lab will be described.
Sheng-Fa Yu (俞聖法)
Sheng-Fa Yu1, Sean J. Elliott2, and Sunney I. Chan1,2
Institutee of Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan1
A.A. Noyse Laboratory of Chemical Physics, California Institute of Technology, Pasadema, CA 91125, U.S.A.2
The particulate methane monooxygenase (pMMO) is a membrane-bound enzyme that catalyzes the conversion of methane to methanol in methanotrophic bacteria. The pMMO isolated from Methyloccocus capsulatus (Bath) consists of a three-subunits hydroxylase (45, 27 and 23 kDa) and an NADH oxidoreductase (38 kDa). The hydroxylase is a copper protein, with 15 copper ions arranged in five trinuclear copper clusters. Two of the copper clusters are associated with the 27 kDa subunit and are involved in the hydroxylation chemistry of the enzyme. The remaining three copper clusters are associated with the 45 kDa subunit, provide a buffer of reducing equivalents in the protein, and participate in electron transfer, mediating the electron input from the NADH reductase to the catalytic clusters. On the basis of chemical and proteolytic treatments, the bulk of the copper ions are localized in the exposed water-soluble domains of the proteins. The remaining copper ions (ca. 6) are sequestered in the membrane-associated domain. The functional form of the enzyme is the fully or partially reduced copper hydroxylase. The pMMO exhibits unusual substrate specificity: only small normal alkanes are hydroxylated and similar alkenes epoxidated. In addition, the chemistry is highly regiospecific as well as stereoselective. It is apparent that the substrate binds to a short and narrow hydrophobic pocket, and the hydroxylation chemistry involves concerted binding of the C-H bond to an activated copper cluster and insertion of the C-H bond to an activate copper cluster and insertion of the "active" oxygen species. Further evidence in support of the stereoselectivity of the hydroxylation chemistry is presently sought by experiments involving a series of designed deuteriated chiral alkanes as substrates. A mechanism for the dioxygen activation mediated by the catalytic clusters will be proposed.
Chun-Hua Hsu (徐俊華)
THE SOLUTION STRUCTURES AND STRUCTURE- FUNCTION STUDIES OF RIBONUCLEASES FROM Rana catesbeiana (BULLFROG) WHICH POSSESS DIFFERENT RIBONUCLEOLYTIC ACTIVITY AND CYTOTOXICITY
Chun-Hua Hsu1,Ning-Yuan Su2,Cindy C-J Kao2,Shih-Hsiung Wu1,3 ,
You-Di Liao2,Chinpan Chen2
[1] Institute of Biochemical Sciences, National Taiwan University, [2] Institute of Biomedical Sciences, [3] Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, R. O. C
The NMR solution structure of RC-RNase, a pyrimidine-guanine sequence specific ribonuclease isolated from Rana catesbeiana (bullfrog) oocytes, was determined two years ago [ref 1,2]. In addition to possessing ribonucleolytic activity, RC-RNase is cytotoxic and can inhibit the growth of tumor cells, which is similar to onconase protein. The unique cytotoxic and antitumor activities of onconase and RC-RNase are now being exploited for their medical applications [ref 3]. Onconase is currently undergoing phase III clinical test as an antitumor drug and the potential of RC-RNase as anticancer drug is also under evaluation. Recently, Dr. You-Di Liao has isolated five more new ribonucleases (~ 105 residues for each protein) from oocyte and liver of Rana catesbeiana and checked their biological functions. These new ribonucleases together with RC-RNase, RNase A as well as Onconase possessed different base specificity, ribonucleolytic activity and cytotoxicity. They all belong to the RNase A superfamily. In order to elucidate the structure-function relationships on these ribonucleases from Rana catesbeiana, several biophysical methods will be applied. First of all, the Molecular Modeling have been carried out. Strikingly, we observe the charge distributions are related to activities of these RNases. Furthermore, CD experiments are used to check the protein stability, secondary structure, structural change at different pHs, and binding studies of ribonuclease with substrate analogues. NMR studies including structure determination, protein folding, and dynamics on these new ribonucleases using native and 15N and/or 13C-labeled proteins are being carried out. The preliminary structural studies based on Modeling, CD, and NMR experiments will be presented in this poster.
1.Chen, C.P., Hom, K., Huang, R.F., Chou, P.J., Liao,Y.D. and Huang, T.H. The secondary structure of a pyrimidine-guaninesequence-specific ribonuclease possessing cytotoxic activity from the oocytesof Rana catesbeiana.
J. Biomol. NMR 8: 331-344, 1996.
2.Chen, C.-F., Chen, C.P., Chen, Y.C., Hom, K., Huang, R.F. and Huang, T.H.. "The Solution Structure of a cytotoxic Ribonuclease from the Oocytes of Rana catesbeina (Bullfrog)". J. Mol. Biol. 283, 231-244 1998.
3. Huang, H.C., Wang, S.C., Leu, Y.J., Lu, S.C. and Liao, Y.D. The Rana catesbeiana rcr gene encodinga cytotoxic ribonuclease : Tissue distribution, cloning, purification,cytotoxicity, and active residues for RNase activity. J. Biol. Chem. 273:6395-6401, 1998.
Wei-Jyun Chien (錢偉鈞)
Wei-Jyun Chien and Gu, Li-Shiou
Department of Applied Chemistry, Chaoyang University of Technology
168 Gefeng E. Rd., Wufeng, Taichung, Taiwan, R.O.C.
Pulsed-field gradient (PFG) NMR spectroscopy has been used to measure the translational diffusion coefficient (Ds) of a cyclic peptide, SRIF14, under anionic micellar environment. Ds value was directly determined by following the decay of the resonance intensity of selected peptide proton. Using a two-state model, the association constant of SRIF14 to the micelle can be estimated. The obtained association constant of SRIF14-micelle binding constant is comparable to those measured from similar systems using other methods.
Impairment of Free Radical Scavenging Enzymes and Enhanced Oxidative Damage in Skin and Muscle Fibroblasts from Patients with the CPEO Syndrome
Ching-You Lu, Edward-K. Wang, Hsin-Chen Lee, and Yau-Huei Wei
Department of Biochemistry and Center for Cellular and Molecular Biology, National Yang-Ming University and Section of Peripheral Neurology, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan
Chronic progressive external ophthalmoplegia (CPEO) syndrome is characterized by ptosis and muscle weakness. Mitochondrial DNA (mtDNA) analysis revealed that CPEO syndrome is associated with large-scale deletions or, less frequently, point mutations of mtDNA in affected tissues of the patients. The molecular mechanism leading to mtDNA mutations is still obscure and the severity and onset age of the CPEO syndrome are poorly correlated with the proportion of mutant mtDNA in the patient's tissues. It is thus of great interest to know whether there are some other defects that are involved in the pathogenesis of CPEO syndrome. Recently, studies from this and other laboratories revealed that an imbalance between free radical scavenging enzymes occurs in a wide spectrum of human diseases and this may contribute directly or indirectly to the pathogenesis of the CPEO syndrome. For studying the alterations of free radical scavenging systems in CPEO syndrome, skin and muscle fibroblasts from patients with CPEO syndrome were cultured. We assayed the enzyme activities of Cu,Zn-SOD, Mn-SOD, catalase and glutathione peroxidase (GPx) of the fibroblasts. The results showed that the skin and muscle fibroblasts from nine CPEO patients all had higher enzyme activities, protein and mRNA levels of Mn-SOD but those of catalase and GPx were not increased or even decreased. These results indicate an imbalance between the H2O2 generation and removal systems in the fibroblasts from the nine patients with CPEO syndrome. Moreover, we monitored the production of superoxide anion and H2O2 by flow cytometry and found that both levels in muscle fibroblasts from CPEO patients were higher than those of healthy controls. The imbalance was much more profound in muscle fibroblasts, which is consistent with the clinical observation that muscle is the main affected tissue of patients with CPEO syndrome. Take together, we suggest that in addition to the impairment of respiratory function the imbalance among free radical scavenging enzymes may play an important role in the pathogenesis and age-dependent progression of the CPEO syndrome.
Huan- shiun Hsu1,3, R. Dayal Yadav1, Pei-Wen Chao1, Shwu-Bin Lin2,Tsung-Mei Chin3, Wei-Chen Lin1, and Lou-sing Kan1
1Institute of Chemistry,Academia Sinica,
2Graduate Institute of Medical Technology, National Taiwan University
3Graduate Institute of Chemistry,Chinese Culture Unveristy, Taipei, Taiwan
The thymidylate synthase (TS) catalyzed reaction is the sole de novo source of methylate of 2'-dexoyurine-5'-monophosphate (dUMP) to thymidine-5'-monophosphate (dTMP). This reaction is required for DNA biosynthesis. Because of its role in cell growth, TS has been a target of antineoplastic chemotherapy. In this paper, we are exploring the inhibition of TS function by antigene triple stranded helix (triplex) formation. We synthesized an oligodeoxyribonucleotides with a sequence in TS mRNA (5'-TTGGGGGGGGACCATGG-3'.(-13~7)) as a target for triplex formation. This motif contains the starting codon of TS. We designed two oligodeoxyribonucleotides, 5'-TCCCCCCCCAA-3' and 5'-CCCCCCCCT-3' for Watson-Crick and Hoogsteen base pairs to the target sequence, respectively. In order to facilitate the triplex formation, we substitute the C in Hoogsteen strand by either 2'-O-methypesudocytidine (O) or 5methycytidine (M). For this reason, two more oligomers, 5'-OOOOOOOOT-3' and 5'MOMOMOMOT-3', were synthesized
The preliminary result showed that melting temperatures (monitored by UV thermodynamic study) of triplex formation are 15 ℃/50 ℃; 24 ℃/49 ℃; and 46.5 ℃/46.5 ℃ for 5'-OOOOOOOOT-3' as the Hoogsteen strand in solution of 0.15M NaC l, 0.02M MgCl2 with buffer at pH 7, 6, and 4, respectively. On the other hand, the melting temperatures are 17℃/50℃; 27℃/48℃; and 45℃/45℃ for 5'-MOMOMOMOT-3' for the same conditions. The triplex Tm(s) are at pH 7,6 and 4 respectively for 5'-OOOOOOOOT-3'. The two-stage melting warranties the triplex formation. The kinetic studies of aforementioned triplexes formation are currently studied by surface Plasmon resonance. (Supported by National Science Council, The Executive Yuan)
Wei-Chen Lin (林維政)
Wei-Chen Lin and Lou-sing Kan
Institute of Chemistry, Academia Sinica, Taipei, Taiwan 11529
A program call SELCON that has been written by Sreerama and Woody in FORTRAN (Anal. Biochem., 209, 32-44 (1993)) is made for the initial guess for the secondary structure of proteins by circular dichroism spectra. This paper presents a detail analysis of SELCON and step by step for how to using it to predict the unknown protein structure. The modified program is available on inquiry and can be run on a IBM or IBM compitable personal computer. (Supported by National Science Council, The Executive Yuan)
Pei-Wen Chao (趙珮雯)
Wei-Chen Lin, R. Dayal Yadav, Pei-Wen Chao, and Lou-sing Kan
Institute of Chemistry, Academic Sinica, Taipei, Taiwan 11529
The Pseudoisocytinide, owing to its potent anti-tumor activity, has attracted both chemists and biologists from the days of its inception. From structurally, it can be represented in two tautomeric form, one featuring an imino group (K1; meaning H is attached to N1) and another one fearing an imido group (K3, H attached to N3). From our theoretic calculation (Nucleosides & Nucleoides, 1999,18,1091-1093.) show that K1 isomer is less stable than K3 isomer by ca. ~2 kcal/mol in solution phase. Thus the proton exchange between the two tautomers is so fast that can not easy to get K1 and K3 separately.
Recently, we established a novel method for synthesis K1 and K3 tautomeric separately. In order to reveal the imido proton exchange mechanism between K1 and K3, the proton and carbon-13 NMR titration experiment for each other have been carried out from pH 1 to 12 in aqueous solution. In contrast, K1 and K3 are not separable which is due to the fast exchange of the imido proton between N1 and N3 sites in aqueous solution, which observations are in agreement with our theoretical calculations. Furthermore, We found that the line broadening of C2 from carbon-13 spectra from pH 3 to 8 range under pH titration experiment. The phenomenon is involved in the imido proton exchange between N1 and N3 sites by through the C2 site. The exchange mechanism of the proton rests at N1 and N3 of pseudoisocytidine would be pointed out in this poster.
Lou-sing Kan (甘魯生)
PROTON NMR STRUCTURAL STUDIES OF 5'-d-(TC)3(CT) 3(AG) 3-3' - A "Paper Clip" TRIPLE STRANDED HELIX WITHOUT LOOPS
Lou-sing Kan1, Laura Pasternack2, Shwu-Bin Lin3, T.-M. Chin4, Wei-Chen Lin1, and Dee-Hua Huang2
1Institute of Chemistry, Academia Sinica, Taipei Taiwan 11529
2Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037
3The Graduate Institute of Medical Technology, National Taiwan University, Taipei, Taiwan 10002
4Institute of Applied Chemistry, Chinese Culture University, Taipei, Taiwan 11114
A DNA octadecamer, 5'-T1C2T3C4T5C6C7T8C9T10C11T12A13G14A15G16A17G18, was found to form a "paper clip" type triple stranded helix (triplex) with the Hoogsteen strand binding in the major groove of the Watson-Crick duplex. Proton NMR studies were conducted in aqueous solution at pH 4.5. We find that the four internal base triads (C2+G14C11, T3A15T10, C4+G16C9, and T5A17T8) form typical pyrimidine-purine-pyrimidine hydrogen bonding configuration except the C2+ base is slight out of the plane. At one end of the triplex, the bases C6+, C7, and G18 form a distorted but stable triad. In contrast, at the other end of the triplex, T12 and A13 form a hairpin turn while T1 is stabilized by its association with T12-A13. This is the first report of a stable triplex formed without loops. (Supported by National Science Council, The Executive Yuan)


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My comment at the Federalist regarding Taxpayer Funding of the Arts
This ARTICLE at the Federalist questioned Taxpayer funding of the arts.
Here is my comment:
"I am involved in theatre and I really believe that it is the lifestyle of the professional actor that mostly contributes to conservatives not participating as much as liberals.
It is nearly impossible for those who want to have a large family to perform eight shows a week and constantly be auditioning and traveling around the country as an equity performer.
I took a 25 year break to raise our five children and recently have joined a local company.
Some of my liberal sister actors have given up family life and had many, many abortions to enable them to live this demanding lifestyle and that is a travesty.
So many friends naturally evolved into gay partnerships because they understood no babies would ever show up to ruin their contracts.
The young actors running the local company I joined are all about "non profit", "tax payer funded", begging the local city government for money, almost at the expense of putting that energy into creating a great show.
I would like to see a new model for professional theatre and opera evolve that is focused on the well being of the actors.
To me they seem to not be aware that a for profit company is even possible. I even get the feeling that they believe it is shameful to be an artist and talk about profits.
Perhaps performing only four shows a week, higher pay for performers, and a more family friendly atmosphere with a thick overlay of traditional values backed up by a free market financial plan.
Instead of a layer of Infrastructure tied to writing grant proposals, the focus would be on the art itself, rather than the constant fund raising."


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It can be tempting to buy a small sized rug and it's not only because they are so much more affordable. Weavers tend to get extra creative when filling up the smaller space and the result can be simply stunning. You just want to own one of those gorgeous pieces and you think you will be able to place it anywhere because it is smaller in size. However, it does not always work that way.
Biggest Design Mistake: Buying Rugs That Are Too Small
One of the biggest mistakes homeowners commit while choosing a rug is buying one that is too small for the selected space. This can make the room look weird and out of proportion. Also, if a rug is too small for the space it loses its charm and appeal.
Small rugs may look fine in a catalog or magazine. However, this is because the decorators or designers accommodate the right type of furniture and décor to make the rug work. This does not hold true in real life where a small-sized rug could be a designing disaster.
For a living room, the smallest rug size should not be less than eight feet x 10 feet, though 9 feet by 12 feet is the perfect size. Only if you have a really compact and small living room should you go in for a rug that is less than 6 feet x 9 feet.
On the other hand, if you go in for a smaller size than this, the place for it would be the bedroom, at the side of the bed and not the living room where it will look awkward and out of place.
Persian Rug Shapes And Placements
Opt for a shape that you like, but do take into consideration the shape of the room and the place where you want to position the rug. It goes without saying that the size of the Persian rug will ultimately depend on the available space.
Also, don't forget that if you can place just one Persian rug in the room then it should cover a minimum of one-third of the floor space.
Persian rugs come in a range of sizes.
Rectangular rugs are versatile and can be placed in almost any space, provided that you choose the right size. You can find rectangular rugs in really large sizes which are ideal for a large floor space.
Long and narrow rugs look great when placed in the hallway, door entrance or the stairways.
Round Persian rugs are also available in a range of sizes. These go well in the living room. Try placing the rug under the coffee table to create a focal point in the center of the room.
If you have a perfectly square room, you may want to choose a square Persian rug. Rectangular rugs do not go well in square shaped rooms.
Handy Rug Laying Hints
Here are some tips for laying a rug in any room:
The rug should not touch the wall. There should be about three to eight inches of space between the wall and the edge of the rug.
If you intend placing the rug in the living, remember a large rug should not extend more than two feet beyond the furniture. It will define the space and help to create a focal point.
If you want the rug to bring the different elements of the room together, ensure that it is big enough to accommodate the front legs of all furniture in the vicinity.
You can place a rug in the dining room. However, it should extend anywhere from 18 inches to 24 inches beyond the dining table.


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The Swarm
In the Geana's lands there is a large collection of insects known as the Swarm. The Swarm ranges over a couple of miles and covers more than 100 square yards at a time. Living beings who come in contact with the Swarm have the flesh flayed from their bodies within moments. Shortly afterward, the meat and blood is devoured, and then only the bones are left. Few living beings have survived direct exposure to the Swarm.
The Swarm is composed of every insect in the land. Some druids on Gaena have, at great risk, catalogued the insects, and they claim there are even insects there that exist no where else in Vernlarum. Some of the creatures are as large as birds and can suck a normal sized humanoid dry of blood in a minute or less. Centipedes of all sizes, and other crawling insects, are a part of the Swarm as well—often finding their way under hastily erected shelters that stop the flying creatures.
Gaena passes through the Swarm once every year or two. The Elders work powerful magics to try to protect the people of Gaena when the Shamble passes through the Swarm. Their spells disguise and conceal those on Gaena, but almost every time Gaena passes through the Swarm, someone is caught out in the Swarm. The animals of Gaena's forests seem to sense the coming of the Swarm, and they seek shelter before they are exposed. With the aid of the Elders' spells, few animals die to the Swarm during a pass.
Some visitors to Gaena have advanced the idea of erecting a magical barrier to keep the insects away or a spell that gives the insects an aversion to Gaena. But the druids understand the necessary role insects play in an ecosystem. While there is something dysfunctional about the Swarm itself, it provides a constant boost to the insect life on Gaena, which helps keep the animal population high and the soil fertile.
The druids have studied the Swarm, knowing that such a large group of insects shouldn't be able to exist the way it does. But nothing they've discovered explains the Swarm's existence to their satisfaction. Now, some just accept the Swarm, others are still curious about the magic that must have been used to create it. Some Acolytes have wondered if the Swarm is an Ark that didn't finish forming at the time of the Creation, though none have been curious enough so far to go to the heart of the Swarm's land. Or if they have, they haven't returned.
One of the Elders, Elurin Oakstaff, has recently fallen ill. He believes he has been poisoned by one of his rivals, but he doesn't know which one. Elurin knows he has a reputation for being a bit paranoid and doesn't expect anyone to believe him. He's also unsure who he can trust, since anyone he asks for help may also be the person who poisoned him or in league with his poisoner. The poison, he believes, is slow acting—the tingling in his extremities started a week ago and has only moved up to his wrists. But he fears once it reaches heart, he will die. Elurin has tried magical and alchemical cures for poison and has yet to find anything that works, which makes him suspect a highly competent, and probably magical, assassin.
Elurin now believes his only hope lies in the Swarm. The gracker bug is a rare insect known only to live in the swirling mass of ravenous insects. Grackers secrete a fluid that is a numbing agent and can be mixed with certain herbs to create the most powerful known antitoxin. Grackers are not particularly dangerous, so Elurin hopes he can find someone to trust, preferably an outsider, who will help him capture the three he needs for his antitoxin. The Swarm is only a few days away—he fears this will be his last chance at a cure. He has means and can pay, and will make antitoxin for those he hires, assuming they recover enough gracker bugs for themselves.


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Non-Dual Practice
Non-dual practice and teachings touch on all aspects of life. They bring about a change in our understanding of our selves and the reality around us.
They are based on the understanding that our true Self is of the nature of pure consciousness, and at one with the Self of all.
The path involves a gradual expansion of our sense of Self, from identification with the body and mind, to the realisation of our identity with pure consciousness.
The more we practice, the more we see. As we see more, the practice becomes natural and rewarding.
In this section there are articles about the non-dual practices and the principles on which they are based. For more on taking them up and establishing our own regular practice see the 'My Practice' section of this website.
The practical teachings as a whole are sometimes known as Adhyatma Yoga, a Sanskrit term meaning the Yoga of the Self.
More on Non-Duality
Discovering Non-Duality
If you are doing some regular meditation and reflection on the non-dual ideas, you are welcome to contact us with any questions about the teachings, or for further suggestions about your practice.


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And you wonder why we are never Here, because we're always Combining Moments. Now a combination of some things works as a conglomeration together, which is for the betterment of that things operating correctly, but rarely realized and not understood, this is not the case with Combining Moments. Moments works best (per se) one after another. I mean the object is to stick yourself Here in This Moment and stay Here until The Moment has ended, which is obviously a Hard thing to do, because we keep on Combining Moments. That's also why we miss out on what is Here and forget things, because once something comes up that we would like to remember, we then bring up a Past Moment, Future Moment, Imaginative Moment or Fantastical Moment, that makes us forget about what we were trying to remember. So the question is; "What is Combining Moments".
Combining Moment is when you are Here present, focusing on doing something, but then you have these thoughts coming up about things you have done in a Past Moment or things you will do in a Future Moment, an Imaginative Moment or Fantastical Moment, which divert your attention from what's in front of you in This Moment, therefore we are not Here present. For example, let's say you were in a conversation with someone and after the conversation, you continued to think about the conversation you just had with that someone and bring it into your Next Moments, as you go about your day, so by still being in that Past Moment, you miss out, mess up or make mistakes in the Present Moment, which all starts with forgetting to realizing that you're breathing and if you're doing something physical like cooking or driving or even walking, that's when accidents happen, where we end up either cutting our finger, running into something or tripping up on that curb we didn't see, because we were busy Combining Moments.
I mean being that we've been Combining Moments for so long throughout our lives, for some of us, it won't be that easy to just be Here in This Moment, it will take practice and understanding what This Moment consist of. Take me for example, I am writing this blog post and once I finish with it, I will post it and after I post it, This Moment will be over. That's what A Moment consist of. Where the problem comes in at is, once I go into the Next Moment, I start thinking about what I wrote and other people in relations to what I wrote, which takes away from the Next Moment I'll experience, so in essence, I am not fully grasping the potential of what the Next Moment will bring, because I am Combining Moments, meaning still stuck in a Past Moment Here in the present.
A common excuse, that I'm sure most of us have used to justify our Combining Moments is; I'm just going over it in my head again, to make sure that I got things right, well maybe so, but we also want to look at the point of being present, which is the defining point of it all, where if we were completely submerged in that one moment, then there would be no question as to our participation within it. Another thing is, if we we're to treat Every Moment the same as The Moment in which we really like doing things, such as our hobbies (for example), then our awareness of and in the present, would be spectacular.
So before I get to the solution, the other evening as I was laying down to rest, I received a phone call from a friend who is currently visiting Hawaii and had a mutual friend on speaker phone, that I hadn't spoken to for a few years, who then started taking and bringing up all of these past memories that I remembered, for the next 30 minutes or so, (until I told them it was past my bed time, lol) after the conversation I then experienced and influx of thoughts/memories about, when I was living in Hawaii and some of the things I did why'll there. Within that, I then noticed an interesting thing, that I couldn't fall back asleep, (after I was woke up from just dosing off) and then it dawned on me how, instead of leaving That Moment in That Moment of conversation, I brought that 'then Past Moment into the New Moment of going to sleep and Combined Moments, which made it hard for me to fall asleep and not only that, the next morning as I was doing my morning routine, what kept coming up was Hawaii and the conversation I had, the night before with these two friend on the phone, and that's when I realized and understood the severity with Combining Moment.
Ok so the reason, I now see that I have not been present at times, (especially alone with myself) is because I throughout my life, have always been Combining Moments, Past Moments as Memories, Future Projected Moments, Imaginative Moments and Fantastical Moments, with and into my Current Moments, where I lose track, mess up or make mistakes, in what I'm doing or supposed to be doing, and in looking at it that way, I am now becoming more aware of walking into the Next Moment and leaving the Previous Moment there, unless I have to Self-Forgive, for the Previous Moment, (Sort of like a reminder to self, "Ok you're going into the Next Moment now", that helps me to stay Here in the present) so for this;
I forgive myself that I have accepted and allowed myself to not realize the main reason for me not being Here, in and as the Present Moments throughout my day, being that I was Combining Moments, from the perspective of bring up Past Moments, Future Moments, Imaginative Moments and Fantastical Moment into the Current Moment, I would be experiencing.
I forgive myself that I have accepted and allowed myself to have not stuck myself in Current Moments and stayed there until Those Moments had ended, but instead find myself drifting into thinking and bringing up Other Moments, that I have and would experience.
I forgive myself that I have accepted and allowed myself to have missed out on what is Here and forgot things, because once something comes up that I would like to remember, I would bring up either a Past Moment, Future Moment, Imaginative Moment or a Fantastical Moment, that would make me forget about what I was trying to remember and/or thinking about.
I forgive myself that I have accepted and allowed myself to have been in a conversation with someone and after the conversation, I continued to think about the conversation I just had with that someone and brought it into my Next Moments as I went throughout my day and have at times missed out on something, messed something up or made a mistake why'll doing something in that Next Moment.
I forgive myself that I have accepted and allowed myself to have after writing a few of my blogs, after That Moment was over, when I posted it, go into the Next Moment thinking about what I wrote and other people in relations to what I wrote, which took away from that Next Moment I would experienced, so in essence I did not fully grasp the potential of what that Next Moment had to offer, and possibly, missed out on a few vital realizations, because I had Combined Moments.
I forgive myself that I have accepted and allowed myself to have used the excuse of I'm just going over in my head again the Past Moment to make sure I got things right, not realizing that the defining point is to be Here present, where if I was completely submerged into that Past Moment at That Moment, then there would be no question as to my participation within it, meaning I wouldn't need to go back over it in my head.
I forgive myself that I haven't accepted and allowed myself to realize that if I was to treat Every Moment the same as The Moment in which I really liked doing things, such as my hobbies (for example), then my awareness of and in the Present Moment, would be spectacular.
I forgive myself that I have accepted and allowed myself to after a conversation I had the other evening over the phone with some friend in Hawaii, bring up all these memories about when I used to live in Hawaii and some of the things I did when I lived there, which then because I Combined Moments, I made it hard for me to go back to sleep, and then only realized the next day as I was doing my morning routine and these thoughts about Hawaii and the phone conversation I had with these two friends kept coming up and distracting me from what I was doing, what I had been doing and why I was always not fully present at that time and times throughout my life.
When and as I see myself Being present in A Moment and find that I am going into my mind and thinking about Past Moments, Future Moment, Imaginative Moments, or Fantastical Moments in Combining Moments, as a distraction from me being Here, where I am not present and could possibly miss out on something, mess something up and/or make a mistake why'll doing something, I stop and breathe. I see/realize/understand that, by Combining Moments like that, I am missing out on the finer things as life, as me and vital realization that are there to assist me to realize myself as everything around me. I also realize that this is detrimental to my physical body, where by not paying attention when doing physical activities, that's when accidents can happen, so it behooves me to remain present Here in this Current Moment. And so, I commit myself to remaining present in every Here moment and no longer accept and allow myself to Combine Past, Future, Imaginative or Fantastical Moments into or within the Present Moments, but to instead keep Each Moment as every breath one after another.


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Webinar Five Steps To A Successful BYOC ProgramAugust 13, 2012 | David K. Johnson
Employees are going in a different direction than most I&O organizations' policies and infrastructure. In this webinar, we present some surprising data to make the case that consumerization...


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How To Tie An Allison Streamer
By Fly Tying Tutorials | Monday, July 25
Tightlinevideos instructional video for tying the best looking fly!
Click Here To Learn More About GuideHire
Already looking for a new fly to tie? Tightlinevideos has you covered with the Allison Streamer, which has to be the best looking fly out there! Almost too good looking to the point where we at GuideHire feel bad letting the fish have their way with it. Although, they are worth the time spent tying because not only do they resemble the artificial intelligent machines in the movie The Matrix, they are also just as killer in the water for reeling in salmon and trout. Matrix references aside, the Allison Streamer was originally created by fly fisherman, Mike Appello, who merged both Gartside's soft hackle streamer and Abramer's flatwing saltwater fly to create what some consider to be "the one" (alright I promise that's the last matrix reference). We at GuideHire always try and give our readers an extra tip to add to the chances of capturing that shameless selfie with the fish, which for many fly fisherman is essential for gaining those instagram followers. So, GuideHire recommends adding junglecock eyes to the allison streamer to taunt those lazy salmon into striking the fly head on instead of letting them get a free nibble at the long tail hackles. Ready, set, Tie!
For our fellow beginner tiers out there, be sure to check out Postfly's Fly Tying Box, and our friends over at Flymen Fishing Co. for specialty hackle and other goodies.


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My Journey at JPL ~ Episode 2: An InSider's View of InSight
Hi Ladies, Welcome to JPL! A few days after I started as an engineer my co-worker gave me this pin. She told... Read More
My Journey at JPL ~ Episode 1
Hi Ladies, It seems like forever since I last posted on my blog. I talked of my experience after seeing Hidden Figures,... Read More


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Photos: SImon Diaz
Lizzo is life.. totally killed it! Check her out on YT
Photos: SImon Diaz
Photos: Jamal Eid
The end of festival season 2016 is fast approaching. We wish we could say that it had all been work, YEAH RIGHT!
Right off the bat, out the gate and first of all BRAVO! I have looked through thousands of images of at least a dozen multi-genre festivals and, HANDS DOWN, MOST DIVERSE GROUP OF ATTENDEES I'VE SEEN EVER!! Body types, ages, race, sexual orientation, cross cultural education, hot, fun people and sexy times!
This album is so THE SHIT, I didn't even have to create a separate featured album!
Dirtybird Campout I wanna go every year!
Images: Simon Diaz
Born and raised in Los Angeles, Hirota made his debut on the fashion scene at the age of 19. He started his first company GLAZA only 2 quarters into attending F.I.D.M in 2002. His first showing in LA Fashion Week resulted in being picked up by destination stores including Patricia Fields NYC, Fred Segal LA, and Volcan & Aphrodite in Tokyo. A decade later Hirota continues his progressive movement with his current company Ashton Michael. Offering exclusive capsule collections available around the globe, wardrobe styling and custom one-of-a-kind pieces combined with artistic vision and a true understanding of fit and fabrication, Hirota creates empowering pieces that test the time not challenged by seasons. Ashton Michael combines goth-grunge with glamour edge and fine tailoring for the all the fashion fearless.
If you have watched the Super Bowl, VMA's, AMA's or any award show video in the past 12 years you've most likely seen the work of fashion designer Ashton Hirota and his label Ashton Michael. Featured on clients ranging from icons of Hollywood to chart topping musicians, Hirota has designed custom pieces for celebrities as diverse as Beyonce, Cher, Will.i.am, Usher, JLO, Chris Brown, Janelle Monae, Miguel, Ciara, and Pitbull just to name a few. Today in addition to designing and producing his own Ashton Michael Label, Hirota is sought out by top stylist for his ability to create fashion forward leather pieces individualized for every clients personality with one of the industries quickest turn around times available. Hirota is a strong believer that there is no gender in creative expression, just individuality.
Ashton Michael Atelier is located in the heart of Hollywood. Available by appointment only to industry professionals, the showroom caters to those looking for unique, one-of-a-kind pieces in addition to current collection items. Purchases and rentals are available with options to alter or customize any piece in house one on one with the designer himself.
Website; https://www.ashtonmichael.com
Social; https://www.instagram.com/ashtonmichael
Social; https://www.facebook.com/ashtonmichaella
Photographer: Jamal Eid
We made it to Woogie Weekend again. Jamal had a blast and was able to capture some beautiful people of color in the crowd and I have highlighted them to the right. Below that you will find the rest of his amazing images.
Images by: Jamal Eid
Farhoud Meybodi
Gabe Ruiz
Live Free Productions
Nomadic Captures
Sarah Perrill
Violet Visions
This is one our favorite festivals. Tickets are on sale and bonus....FLASH SALE Today and tomorrow
Photos by: Cameron Jordan, Carolyn Crafty, Juliana Bernstein, Aaron Glassman, Andre Schnyder, Dan Krauss
Photos by: Jamal Eid
Nocturnal Wonderland celebrated its 20th Anniversary this year. Some of the bigger Dj's in attendance included Z Trip, DJ Jazzy Jeff, Beat Junkies, Donald Glaude, Afrojack, Kaskade, Yellow Claw and Alesso.
To be quite honest, it really felt like the only DJ's that didn't show were probably due to scheduling conflicts. This party was jam fucking packed!
Photos by: Jamal Eid
By: Jeremiah Alexis
My fave transformational festival Lightning In a Bottle just released their after movie and man does it bring back good memories. As we chronicled with our in depth reportage two months ago HERE LIB remains our favorite fest and a shining example of how a TF should be done!
Not only featuring a stellar line up with acts like Odesza, Flume and SBTRKT, LIB is also the most environmentally conscious festival in America, having received the Outstanding Award four years running from A Greener Festival for its healthy food vendors, free water stations, composting and recycling infrastructure, pack-it-in/pack-it-out policy, and other green initiatives. Definitly check it out in 2016, as it might possibly change your life!
Check out the next Do Lab co-produced event Dirtybird Campout taking place this October in Silverado, California. Simulating a summer camp vibe, there will be great music along with Kickball competitions, Egg Toss, Capture the Flag, Color Wars, Kickball, Late Night Bingo and much more! Can't wait, we will certainly see you all there!
written by: Jeremiah Alexis
photos by: Johnathan Leyva
Lightning In a Bottle 2015 changed my life and the lives of the other 20,000 souls that were also along for the ride. Whether you were meditating at the Temple of Conciousness every day, doing yoga at sunrise or simply partying your face off for 4 days, I can say definitively, it changed your life.
By Jeremiah Alexis
symbols (deVour) readers knows that we here LOVE our transformational festivals! So we were very excited to receive exclusive notice of a new TF entrant into the arena, Further Future. Based out of the Las Vegas area, and voted Best LV Festival 2015 by Las Vegas Weekly (beating out Electric Daisy Carnival, etc), Further Future brings over ground and underground dance vibes along with an amazing caliber of speakers. Last year including musical guests as varied as Warpaint to Com Truise, speakers such as SoundCloud CEO/Founder, Alex Ljung and Google[x]'s Captain of Moonshots, Dr. Astro Teller.
Look out for Further Future 002 in Spring 2016. They will be sharing further glimpses into the Further Future experience in the coming weeks with video from artist performances, workshops, and speaker sessions. Checkout last years vibes from their just released highlight video from this years fest. See you there in 2016!
Photos by: Simon Diaz
Written By: Bellisima
Life is beautiful, isn't it? For those with a strong passion for enjoying life, and all encompassed, we recognize and appreciate that which brings us happiness. And for those of us who live and breathe our passions, I'm sure nothing gets the juices flowing more than actively engaging in them; making memories, living life to the fullest, contributing to this beautiful world, making our mark, inspiring others.
...And that is exactly what the "Life Is Beautiful" festival was all about. LIB pairs complementary ingredients for the perfect recipe, bringing a fun and innovative twist to the standard festival. Actually, "fun" is a languid noun, overshadowed by what this festival is known as - and honestly, this event truly is - EPIC!
This is the festival that I had been looking forward to for since I started my festival culture journey. I had the most amazing time at Lucidity and since these two festivals are related, this one was just as transformative. The culture at these festivals is swathed in Love, Art and the most interesting and creative fashion. symbols (deVour) was proud to share all that this festival had to offer from workshops to fashion to music.
Facebook: www.facebook.com/onceuponafest
Instagram: @onceuponafest
Twitter: @OnceUponAFest
Photos: Zac Krohn of Zestful Imaging
HARD Summer 2015 in Pictures. Be sure to check out Interviews by Jeremiah Alexis for Peking Duk, Golden Features and DirtyPhonics.
Photos by: Jamal Eid
If Peking Duk's Adam Hyde and Reuben Styles hadn't left the world of rock and hip hop as youth's, they would almost certainly be spitting in your face rhymes about partying or head banging till their necks gave out. Instead these two fine Aussie lads are setting turntables ablaze around the world as pre eminent party starters and truth talkers who have taken over OZ and are now setting their sites on the states. I was privileged to chat with them about their refreshingly honest opinions (Including which famous DJ is a Fuck Boy) and 15 minutes later we somehow started on a path to creating a new religion and taking over the world. Check it out!
By: Jeremiah Alexis
Photos by: Jamal Eid
When the first Virgin Galactic flight takes off for it's maiden voyage into space, Golden Features new EP XXIV might be the perfect soundtrack. Jangly bass, dark synth stabs and ghostly voices float over a persistent house beat keeping your booty moving through the galactic mist. I was very excited to check out Golden Features aka 24 year old producer/DJ Tom Stell at his very first American festival set at HARD Summer. He didn't disappoint as he played a very ethereal but body rocking set mixing almost all his own tunes, which is a rarity for such a new artist. Soon I found myself in a barn with the enigmatic Mr. Stell and we got into a brief but compelling conversation on his artistic origins, the burgeoning Sydney electronic scene and his potential beef with one of hip hop's first anonymous rappers.
By: Jeremiah Alexis
Photos by: Jamal Eid
Dirtyphonics are a Parisian DJ/Producing trio consisting of Pitchin, Charly and Pho. They had been going Hard in the French indie EDM scene for years, before signing with AudioPorn records and releasing their first official single "French Fuck" which went to number 1 on Beatport. Since then they have toured the world, signed onto Steve Aoki's Dim Mak Records and just recently lost an original member. We sat down with them at Hard Summer to get the scoop on their creation process, their weirdest gig and the emotions that come with a missing band mate.
By: Jeremiah Alexis
Photos by: Jamal Eid
The Mercadantes present: Breath - A Collection Film
Director: Daniel Mercadante
Executive Producer: Katina Mercadante
Producer: Jed Hubbard
Music: Keith Kenniff
Associate Producers: Jordan Shively, Caroline Kousidonis
Production Associates. David Duhl-Coughlin, Alisha Shimada
In this new experimental short film "Breath", award winning directing duo The Mercadantes explore the way one breath encapsulates a single moment as well as the invisible life force we all share.
Filled with sighs, kisses, coughs, breathless moments, baby's first gasps and early morning inhales, 'Breath' explores the poetry and sensual underpinnings of "life in its simplest forms."
"It's a matter of noticing," says Daniel. "To get people to notice life in its simplest forms. That can be truly beautiful."
As poetic as it is, "Breath" is not without a unifying theme. "The take away is about humanity, how everything is connected," Daniel adds.
About The Mercadantes...
The Mercadantes have worked primarily with non-actors to capture, rather than construct, their subjects and their stories. The duo's shorts and spots reveal the beauty in the everyday, playing with the lines between documentary, fiction and experimental cinema.
Along with broadcast and online commercials for brands such as Facebook, Apple and VW, the directors' films have screened at art galleries including the Guggenheim's YouTube Play Biennial. The Mercadantes' film work has won numerous awards including a 2015 DGA Award nomination, Clio Awards, Cannes Lions, and inclusion in Ted Ads Worth Spreading.
Daniel Mercadante entered the directing spotlight with the Everynone films Symmetry and Words, commissioned by the wildly popular NPR show/podcast, Radiolab. The episodes garnered millions of views online, inspiring agencies and brands to tap the director's unique talents. Katina Mercadante directed and produced documentaries in Africa, Asia, Europe and the Americas, working on international film projects for the UN. Educated at Amherst College, she met Daniel while making a series of commercials shot in a documentary style. The Mill Valley-based duo has collaborated on films and award-winning commercials ever since, and recently joined Oscar-and-Emmy winning production company Park Pictures.


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Instruction to Authors
The journal publishes original research papers on all aspects of agriculture and food science relevant to the tropical and subtropical regions. The journal also publishes short communications.
Papers published or under consideration elsewhere will not be accepted, but publication elsewhere of an abstract or extended summary does not preclude publication in full in this journal.
All papers are expected to be peer-reviewed, before submission, not only for scientific content, but also for correctness in presentation of text, references and illustrations. All authors are responsible for any paper they co-authored.
Submitted papers will normally be sent to two subject matter specialist as referees (in Malaysia and/or overseas) for constructive confidential appraisal, to maintain a high standard of publication.
Well-presented papers result in faster turn around times, more accurate proofing and reduced time to eventual publication. Paper submitted in poor conformity with the editorial requirements given below may be returned without review.
Date of acceptance is the date the author's final revision is officially accepted by the Editorial Committee.
No printed off prints of the published paper are provided. Authors may request for the PDF copy of their paper.


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PRMG Retail Further Expands its West Coast Region and is Open for Business in Highland, CA!/
California-based mortgage lender, Paramount Residential Mortgage Group, Inc. (PRMG), is expanding its West Coast region by opening their new retail location at 7843 Church Street, Highland, CA 92346.
The newest location will be managed by thirty-nine-year mortgage veteran; Kirk Stoffel. Kirk has worked as a Branch Manager with Gold Star Mortgage, Mountain West Financial, and Golden Empire Mortgage, to name a few.
"The truly visionary leadership team and exceptional operations personnel in place at PRMG gives me the confidence to be able to improve upon the service for my referral partners and their clients. I believe financial wealth starts with Home Ownership. I believe progress does require change and PRMG has all the tools I need to help people achieve their dreams. Thirty-nine years in the business has taught me that changes will come and I know that PRMG will allow me to expand my services to meet those changes and provide the service my clients will need to succeed. What we do has an impact on future generations. Everyone I have met at PRMG has a sincere desire to help people reach their home ownership goals. That is unique and I believe it is the key to their amazing operation", said Stoffel.
Kirk will work closely with PRMG Regional Manager; Marlene Hoover and will have the support of PRMG SVP, Director of National Retail Production; Chris Sorensen.
The new PRMG Highland location is looking for Motivated Loan Originators to support the PRMG mission to being "Progressively Better in All that We Do". If you're ready to join a top-tier team in PRMG's newest San Bernardino County office, please contact Kirk Stoffel at 703.272.4721 or firstname.lastname@example.com.


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SSAT Masterclass: Astronaut Leadership
- This event has passed.
SSAT Masterclass: Astronaut Leadership will be led by six times astronaut Dr Mike Foale (CBE) and Chris Barber, founder of International Space School Education Trust (ISSET). You will discover what it takes to lead in space and how those practices can be applied in your own leadership.
Mike and Chris will help unpack some of the transferable leadership lessons from their incredible space missions and experiences, including Mike's time as Commander of the International Space Station (ISS). Themes covered during the day will include leadership and followership, decision making, team building, change management and learning from failure.
This event is open to any school leader seeking fresh ideas, including senior leaders, directors of teaching schools, headteachers, executive headteachers and chief executives who help to lead whole-school and multi-school improvement.


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South Florida
The weather is nice and the price is even nicer — enjoy National Parks for free
It's basically summer in Miami, and the best way to celebrate is to go outside.
Need even more incentive? It's National Parks Week, so entrance to any National Park is free this weekend and next (April 15, 16, 22 and 23).
Go for a hike, a swim, a bike ride. Try star gazing, fishing or camping. Whatever you like to do in a park, it's free to walk in.
For South Floridians, the best bets are Everglades National Park and Biscayne National Park. Head to the Keys for Dry Tortugas National Park, or drive west for Big Cypress National Preserve.
For more information, check out findyourpark.com
If you missed out on the free days, or you'd like to stay closer to home, Miami.com has outdoor options galore.
Read More: Where to go for outdoor fun in Miami


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Before & After Pictures Dental Implants Images
Before and After pictures are a major testament to a dentist's skill. Chances are that if you like the results in a dentist's before and after pictures, you will be pleased with your own results. Below is a sampling of Dr. Anthony Mobasser's treatment using implants to reconstruct the collapsed bite and restore the missing teeth, self confidence, and self esteem. Patient walked out of our office on top of the world!
It is amazing having a beautiful Smile, custom designed by Dr Mobasser can do!
Before placing dental implants for back teeth
After placing dental implants for back teeth
Dental implants in combination with reconstructive dentistry were used to give this patient his confidence and self esteem back.The color and the shape of the teeth was altered throughout, giving the patient a younger looking smile. Dr. Mobasser and his elite team designed the smile makeover to make the patient feel complete. The compliments patient received after his treatment was priceless.
Dental Implant Bridge for missing teeth – Before & After:
Front Tooth Implant – Before & After
Zerconia implant was used for front tooth, but in order to match the color to the adjacent front tooth, Emax crown in combination of feldspathic porcelain was used to restore the tooth implant. Notice the symmetry, shape and color, our patient was so happy and the result was priceless.


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By Toon Army
April 7 2015
Another ridiculous derby performance as the entire management and playing staff looked like they had no idea what they were doing.Five successive derby humiliations give the clearest of indications that things are seriously wrong with the club at every level. Local lads were present on the pitch, with Sammy Ameobi, Jack Colback and Adam Armstrong all too aware of what was at stake today but a team run by a Dutchman, with a few born-and bred mackems showing a pride and passion for the shirt that was sadly absent from the visitors.
Quality is lacking all over the pitch, in the dugout and in the boardroom and nothing can hide that as the relegation-threatened red and whites managed to secure another 6 points from us, points that will effectively save their skins again. The strike that won this encounter on a sunny Sunday afternoon was admittedly stunning but it was struck by a proven Premier League goalscorer.
United rolled up with a line-up that included too many players who are simply not good enough and players who are playing out of position. It's simply not acceptable and a healthy-looking balance sheet only makes it harder to stomach. If United had won this game by two goals the mackems would have been put in the bottom three. Sadly it was a scenario that never looked remotely likely.
Chants of "You don't know what you're doing" were sung towards the end of the game and the players were booed as they went towards the away followers, leaving the field to a rendition of "You're not fit to wear the shirt". Absolutely tragic - but all avoidable and totally self-inflicted, in pursuit of a favourable balance sheet.


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Topic created by Tom-O on Thu May 10, 2018 at 8:13 pm
Num Posts: 55
Primary Club: DVOA
Fav map: Egypt Mills
First O: 1979
The 2018 DVOA Summer Picnic and Vice President's Cup will be held Sunday June 10th at Fort Washington State Park. The event center will be Pavilion #1 which is located adjacent to Parking Lot #1 in the Militia Hill section of the park - follow O-signs from the park entrance off Militia Hill Rd. Registration will open at 10:00 am with a mass start relay beginning at 11:00 am.
The relay consists of three legs:
White 1.7 km
Yellow 1.8 km
Brown 2.7 km
A regular White Course (2.1 km) will also be offered from 10:00-12:00 for those not participating in the relay. In an effort to balance teams for the relay, a point system will be used. Official relay teams must have a minimum of 3 points. Points are assigned as follows:
1 point: Junior (20 and younger)
1 point: Veteran (40 and older)
1 point: Female
A single person can count for 2 points if they satisfy two of the criteria above. If you know your relay team in advance, please send the information (names, point info) to toverbaugh at comcast dot net. If you don't have a relay team and would like us to assign you to a team, you can either email me your info ahead of time or let us know at registration the day of the event. Relays are an exciting and fun format. The more teams we have participating, the better it will be.
After the conclusion of the relay, we will have lunch in the pavilion. Please bring a dish to share. The club will provide grilled burgers, veggie burgers, and hot dogs. After lunch, we will hold the annual summer meeting.
Don't miss this opportunity to enjoy some (almost) summer orienteering and socialize with other DVOA members!
Tom Overbaugh
Reply by BRycharski on Wed May 16, 2018 at 9:11 pm
Num Posts: 179
Primary Club: DVOA
Fav map: Rutgers Preserve
First O: 1976
OK I will bite tom's team formation statements with an observation. That I guess means that two 34 year old DVOA males (0 points) whom form a team (of 3) with an expectant mother-to-be (has doctors OK note), she's aged 44, is that a official DVOA 3 point relay team? It does satisfy all the rules, right (1 point for the under 20 kid concept, 1 for F 40, and 1 point for over 40)? :-) Are there variations allowed? How about 1 point for all three members being total strangers to each other (each live say in Ct, NJ and PA), and are assigned at random by the CS or Tom? Should there be incentive for more between 21 an under 40 members be rigged, Ahem I mean included? Might increase attendance IMO, using my Robert's Rules copy. Besides the obvious that F 15's et. al., carry a sexist 2 point bonus the M 15(under 20 set) males only have 1 point, a bit of a downer for us younger set of youths. Can' we buy 1 point(s) for cash charity donation to the DVOA Jr. team trainers fund? a potential fundraiser here ...
Reply by Tom-O on Wed May 16, 2018 at 9:30 pm
Num Posts: 55
Primary Club: DVOA
Fav map: Egypt Mills
First O: 1979
Bob,No variations allowed. If people want to form teams that don't meet the 3 point criteria, they can run as an unofficial team.


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Useful information for a trip to Zimbabwe
Zimbabwe is a country that has the potential to satisfy almost everyone. It can offer unspoiled wilderness of the park Mana Pools (near the Zambezi River), thundering masses of water of Victoria Falls, the ruins of Great Zimbabwe, the largest historic complex build of stone located south of the Sahara, or the natural beauty of Nyanga Mountains in the east of the country near the border with Mozambique, or the wild life sanctuary Matopos Hills in the south.
The offer of breathtaking attractions is also enhanced by the favorable climate. Although the country lies in the tropic zone, a high average altitude guarantees a pleasant almost year-round warm weather comparable to the European summer. The warm and humid period falls on the local summer running from November to May, while the winter and cooler dry season lasts from May to October.
The political situation
The main reason that tourists are discouraged from visiting this exotic land of Zimbabwe, remains the political situation. Under the autocratic government of President Robert Mugabe the country has experienced an almost economic free fall in the last decade, which was accompanied by the world's highest hyperinflation of the 21st century. Economic decline aroused the political opposition who despite all persecution of Mugabe´s regime won the last elections. But that Mugabe shrewdly solved the issue by bribing the victorious opposition share of the state power, and continues to be the president of the country.
Presidential elections should be held this year again, but the term was not announced yet and the government excuse themselves that "there is not enough money for the organization of elections in the state budget".
If you are considering a trip to Zimbabwe it is a good idea to monitor the political situation, because the period around the elections is certainly not a good time to travel around the country or even just visiting the capital city of Harare.
Visa to enter the country
Citizens of the Czech Republic need a visa to enter Zimbabwe. Tourist visas can be obtained at any embassy of Zimbabwe or directly at the border when entering the country (although having a visa at the border is in operation since 2007, it is better to always check this information before traveling). Applications for other than a tourist visa must always be administered through the Embassies. The nearest Embassies of Zimbabwe are in Vienna and in Berlin; Zimbabwe has no Embassy in the Czech Republic
Zimbabwe Embassy in Vienna
Botschaft der Republik Simbabwe in Wien
Neustift am Walde 91
1190 Wien
tel.: +43/1/407236/7
fax: +43/1/4079238
e-mail: email@example.com
Zimbabwe Embassy in Berlin
Die Botschaft der Republik Simbabwe in Berlin
Kommandantenstrasse 80
10117 Berlin
tel.: +49/30/206 22 63
fax: +49/30/204 55 062
e-Mail: email@example.com
Fees for visas
The fee for a visa for a single entry is 30 USD (valid for 30 days), double entry visa fee is 45 USD, multiple entry visa is USD 55.
Zimbabwe abolished its own currency – the Zimbabwean dollar which plagued the skyrocketing inflation in January 2009. Everywhere accepted in Zimbabwe now are US Dollars (USD) or South African Rands.
Almost everyone speaks English in Zimbabwe, although English is not the native language for the majority of Zimbabweans. Local languages are Shona – especially in the east, north and center of Zimbabwe and Ndebele in the west of Zimbabwe.
There is no compulsory vaccination when traveling to Zimbabwe but the vaccination against yellow fever is recommended (it is compulsory only when coming from countries where yellow fever occurs), as well as against meningococcal encephalitis, and hepatitis A and B
Health situation
Zimbabwe is among the African countries with the largest expansion of AIDS. Up to 35% of the population is infected in some areas. In low-lying areas (Victoria Falls, Lake Kariba) there is a risk of malaria but in general the level of malaria occurrences very low in Zimbabwe and malaria does not occur at all in the dry season.
Dangers during traveling
Traveling is generally safe in Zimbabwe. Try to avoid street crime in larger cities (risk of pickpocketing or theft of baggage or electronics).
What to bring?
A good aid for traveling will be a plug adapter of the British system, a flashlight in case of power failure or absence of electricity in rural areas, and sun lotion with a high protection factor.


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< Volver
Exceleron Software: Director of Software Engineering
Publicado 08/02/2019
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Dallas, TX
Rapidly growing high-tech Software as a Service (SaaS) Company located in Dallas, TX is looking to expand its Engineering organization with the addition of a Director of Software Engineering with a minimum of 7 years of Perl development experience and a minimum of 5 years of leading development teams. Must be capable of developing a working understanding of the Exceleron's MyUsage software which is used to support prepay accounts, usage monitoring, customer communications and meter data management applications associated with electric, water and gas utilities. The ideal candidate will have a strong background using the Catalyst Web Framework and significant experience implementing Javascript, CSS and HTML with Perl. Flexibility, a strong teamwork approach, and the desire to work in a fast-paced environment are necessary for success. The position will report to the Chief Technology Officer and manage members of the Engineering team. The company offers a competitive salary, fun work environment and a benefits package. Candidates may work remotely with some light travel to the Dallas office (i.e. a couple of days each quarter). Candidates must be legally authorized to work in the United States and must pass a background check.
Design, implement and deliver product releases to Utility focused SaaS product
Technical leadership of 20 developers located in the U.S. and offshore
Work with business and process management teams to deliver well-tested software releases on-time into our highly available production environments
Required Qualifications:
5+ years of experience leading both onshore and offshore development teams with ownership of large web projects
7+ years of experience with Perl
5+ years of experience with Catalyst Web Framework
7+ years of experience with Javascript, CSS, and HTML
Experience in server-side programming languages such as Python and C/C++
Strong hands-on experience in building and maintaining production tools
Experience in developing tools for system configuration, deployment, and monitoring
Strong experience with different RDBMS and NoSQL Databases
Experience in benchmarking, analyzing, and root cause analysis of operating system, databases, and application code
The ability to work and handle complex assignments with a high degree of independence
Candidate should be driven to learn outside of work environment, with a spirit of self-improvement and desire to stay up-to-date with new frameworks
Highly Desired Qualifications:
Experience with continuous integration tools such as Jenkins repositories
Strong Linux/UNIX systems and network application and design experience
SOAP API development experience
DevOps experience
To apply:
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2 walnut benches in progress
The underside of these two imperfect walnut benches are exposed in this in progress pic. Of six or so benches we made recently out of these heavy 13" walnut beams, this imperfect one is clearly my fav. Love the balance of the rotten imperfect beam with the simple lines of the two feet. Just perfect really. You'll see these two benches complete and ready for your butt at this summer's Atlanta Furnishings Market in July.