text:
Fetish injection medical video
LAKEISHA KERRYTuesday, November 26, 2019 12:47:02 AM
One of the sub-types of medical fetishism comprises individuals who derive sexual pleasure and arousal from being the recipients of a medical or clinical procedure typically some kind of bodily examination. This includes genital and urological examinations e.
Obviously this fetish appears to be a very niche sexual behaviour within medical fetishism but there are various online forums and websites that cater for individuals who derive sexual pleasure from the Fetish injection medical video or receiving of injections or watching such acts. For instance, there is a dedicated forum within the Voy. In researching this article I came across many online accounts of various degrees of detail of people claiming to have an injection fetish.
Unlike the others quoted here, this last extract is from a person also provided further description about himself. He was 39 years of age when he posted his comments and claimed to have developed the fetish in childhood some time between the ages of six to eight years. Although the accounts here are brief, all five are males, and three of the five extracts mention getting an injection from a nurse at some point on their lives had kick-started their injection fetish and would appear to suggest that associative pairing took place and that their sexual arousal from injections arises as a result of classical conditioning.
In the film
Fetish injection medical video, a doctor has sex with a woman after she has been given a lethal injection and arguably is more about necrophilia and lust murders than it is about injection fetishes.
Allen Bartholomew published back in in the Australian and New Zealand Journal of Psychiatry alluded to behaviours that have similarities to injection fetishes. Bartholomew was studying the characteristics of intravenous drug users and noted three cases of autohaemofetishism i.
In both of these two features, it was argued by Bartholomew that both of the two features were considered to be brought about by "Fetish injection medical video" conditioning.
More recently, in issue of the journal Rhizomes in Emerging KnowledgeDr. Varpu Rantala examined the recurrence of drug injection scenes in contemporary mainstream cinema from a cultural studies perspective. She argued that in cinematic terms:. She also makes reference to the portrayal of drug addicts in the work of US writer William Burroughs.
Again, this is not used in a sexual sense but she does make some interesting observations about obsession and addiction:. These images may also be fetishized.
The recurrence of these images in their over-indulgence of sensuous material of extreme explicitness reminds one of the processes of addiction as unwilled repetition of excessive sensual experience: This may also be about an intense encounter that moves us.
Finally, and staying with filmsa few years ago there was an interesting article on the Hannibal Studio "Fetish injection medical video" website a site dedicated to critical analysis of all things Hannibal Lecter.
Lecter to Clarice Starling. The essence of those injections, which would lead her from one life to another and help her cross the final threshold to her transformation. So what do you think is the significance of injections according to the Harris realm? Two features occasionally associated with intravenous drug users: Australian and New Zealand Journal of Psychiatry7 3 Cinematic heroin and narcotic modernity.
An International Annual of Critical Aesthetics pp. Images of the desire for drugs. Health Sociology Review, 12 2 Towards an holistic understanding of injecting drug use: An overview of needle fixation. Addiction Research and Theory, 9, Schizoanalysis as audiovisual thinking of cinematic drug injection images. Cultural Studies in Emerging Knowledge24, Gender, genre and excess. Film Quarterly, 44 4 Collapsing the two injection-as-sexual fetish and injection-as-drug fetish concepts; with the rise of more extreme sexualised drug use among gay men, some of which involves injecting crystal methamphetamine, there has been the emergence of fetish and pornography centred specifically around meth injection, either with or without subsequent sex.
You are commenting using your WordPress. You are commenting using your Twitter account. You are commenting Fetish injection medical video your Facebook account.
Notify me of new comments via email. Welcome to my blog! Skip to navigation Skip to main content Skip to primary sidebar Skip to secondary sidebar Skip to footer drmarkgriffiths Just another WordPress. Getting to the point: I had to [have an] injection every day [from a] nurse…On [the] first two days she told me to lower my pants [to give the] injection.
On [the] third day I told her to [take] down my jeans by herself. First she hesitated, but [did] it. I could not turn her down when she said we must go somewhere and get it on…I have
Fetish injection medical video felt so satisfied after she [injected] me. She was forceful and demanding. The [injection] shot was large and scary. So I bent over. I was a bit resistant.
She was persuasive in her words…It was hurting. Then while she was injecting that was hurting too.
I was squirming and moaning. That means that I get only sexually attracted when thinking about women getting injections in their butt. I also like to have fantasies about myself getting injections in the butt by woman. This fetish is apparently rare, but also not that uncommon…As such, a fetish might not be something bad, but this one prevents me from having orgasm in normal sexual intercourse. She argued that in cinematic terms: Again, this is not used in a sexual sense but she does make some interesting observations about obsession and addiction: Cultural Studies in Emerging Knowledge24, Wikipedia He has published over research papers, five books, over book chapters, and over other articles.
He has served on numerous national and international committees e.
Fetish injection medical video also does a lot of freelance journalism and has appeared on over radio and television programmes since Leave a comment Comments 1. July 29, at 9: Leave a Reply Cancel reply Enter your comment here Fill in your details below or click an icon to log in: Email required Address never made public.
Veja Medical Fetish Butt Injection vídeos porno gay gratuitamente, aqui no mkbagsoutlets.info Ordene os filmes por Mais relevantes Fetish injection medical video veja já os melhores Medical. Watch free medical injection fetish videos at Heavy-R, a completely free porn tube Fetish injection medical video the world's most hardcore porn videos. New videos about medical. One of the sub-types of medical fetishism comprises individuals who website ( featuring lots of medical fetish videos including injections), and.
MORE: Leotard and tights fetish
MORE: Embarrassing female examination fetish
MORE: London fetish shopping london


text:
In addition, native advertising platform Nativo is the first to partner with DoubleVerify on its new native viewability offering. Nativo is also now certified by DoubleVerify on its Exchange.
Native advertising is poised to capture even more of marketers' media budgets -- projected to capture 74% of all ad revenue by 2021, according to a bullish Business Insiderreport.
DoubleVerify said that as native advertising continues to claim an increasing portion of budgets, the need to verify viewability is imperative. The challenge? The editorial nature of native ads that makes them effective also makes them challenging to measure. DoubleVerify said that native ad executions differ from standard Interactive Advertising Bureau units in dynamic size variation, content orientation, and the way the units load and render. These factors create complexity when measuring viewability for the format.
DoubleVerify and Nativo collaborated to tackle these challenges in developing a native viewability measurement solution. The solution extends DoubleVerify's proprietary Authentic Impression digital ad quality measurement, which assesses the quality of and engagement generated by native ads, ensuring they are brand-safe, fraud-free, and viewable.
Nativo has been an active promoter of guaranteed native ad viewability with its Viewable CPM (vCPM) metric ,which aims to offer more efficiency to digital advertisers, according to Chris Rooke, SVP of strategy and operations at Nativo.
"Viewability should be table stakes for any digital campaign now, but the numerous variations in format type, size, and content rendering add layers of measurement complexity for both brands and publishers," Rooke told Real-Time Daily via email. "Due to this complexity, collaborating with DoubleVerify to get the measurement right was important and will benefit the industry as advertisers increasingly embrace native formats."

subset: commoncrawl


text:
Foothills Cottage Cardigan
sku: 9794-S
style: classic oversized cream boxy puff sleeve cardigan sweater
vintage / modern: Modern
We only have 0 left in stock!
Classic vintage inspired oversized cardigan in cream off white hue. This cardigan features long puff sleeves, v-neckline, three large buttons down the front, and front patch pockets.
- s: 22.5" length /open bust / 24" sleeve (from drop down shoulder)
- m: 23" length / open bust / 25" sleeve (from drop down shoulder)
- l: 23.5" length / open bust / 26" sleeve (from drop down shoulder)
- material: 62% polyester 27% acrylic 11% viscose
- care: hand wash cold / lay flat to dry
- imported
FREE Domestic Shipping on orders $150+
Payments: All major credit cards, Paypal, and Amazon Pay
48 Hour Returns and Exchanges (Store Credit)
For more detailed information about all of our Shop Policies, please visit: Customer Support
We take extra care to provide detailed measurements for each of our garments for your convenience. If you have any questions or concerns regarding fit, please contact us and we will be happy to assist you. We measure each garment hanging on a hanger.
Length: Top of shoulder to hem
Bust: Armpit to armpit then doubled
Waist: Across natural waist seam then doubled
Hips: Measured 7" from natural waist seam, measured across then doubled
Bodice: Top of shoulder to natural waist seam
From over a decade's worth of experience as a vintage clothing seller and collector, we are sharing some general care and laundering advice gleaned from women of the past. Practicing these "vintage" methods is another way to be more sustainable.
- Wear a slip with your garments to keep your garments cleaner longer (in most cases it takes much less water/resources to launder a slip than it does a garment)
- Wear your garments more than once before laundering
- Use cold water to launder your garments, hot water can deconstruct the fibers of your garments more and also can cause shrinkage.
- If using a machine, only wash full loads of laundry
- Hang garments to dry (every Adored Vintage girl loves seeing clothes on a line!) Dryers can really be rough on your garments (that lint trap is literally bits of your clothes as it slowly falls apart!)
- Spot clean garments immediately
- Hand wash your clothing as often as possible.
- Learn simple repair and mend skills to increase the longevity of your garments.


text:
Invitation For Baby Boy Shower
Invitation for baby boy shower from images na is graceful ideas which can be applied into your baby shower invitation design 1
We are all familiar with the current invitation design. But have you ever thought about something different, bold, unique and like no other? And in here, I would like to share the collections of invitation for baby boy shower, all of them are definitely worth your attention. This post#2844 will inspire you with 5 awesome ideas for your baby shower invitation with a various styles and trends.
Read More
This post#2844 is being packed with 5 cool design gallery. So, we hope this invitation for baby boy shower will give you an extra ideas to make your own baby shower invitation! Look how to apply these and other smart ideas below!


text:
Airsoft Innovations
40 Mike Grenade
Specna Arms BBs
Thunder B Shell & Co2 Only €2.50
Click for more info on The Honour Patch
Play Safe
Play With Honour
Respect The Law
In stock
Price incl. VAT.
Airsoft Innovations 40 Mike is the most powerful 40mm gas grenade ever made! It features a range of a 100ft,
150BB capacity and fires every shot at 240fps using Airsoft Innovations patented Quantum Drive Technology.


text:
also known as schorl, this powerhouse of a stone is extremely grounding, protecting, and realigning.
it is correlated with the root chakra, because it is very anchoring and stabilizing, rooting you deeply into the present moment and into the physical, earth plane.
this powerful, recharging crystal is strong and effective in removing negative energies, and quickly dissolves worries and anxieties. works like an energetic vacuum cleaner; bringing about a protective energetic barrier or boundary to the aura, while also transmuting and dispelling any of the negative energies surrounding. also protects from electromagnetic frequencies.
it carries a subtle, but consistent, electric charge, which is why it is known as the "electric stone". black tourmaline is also incredibly rich in iron, and can help to soothe and settle the bones and muscles.
fun fact : this stone was known to be used by ancient magicians throughout the ages to protect against negative entities and "earth demons" as they cast their spells.


text:
Alternative Titles
Synonyms: Katteni Kaizou, Yuuryoubukken Chuukaishimasu, A Brooding, Gloomy Property, Yuuryou Bukken Chuukai Shimasu
Japanese: かってに改蔵
Type: Manga
Volumes: 26
Chapters: 289
Status: Finished
Published: Jan 18, 1999 to 2004
Authors: Kumeta, Kouji (Story & Art)
Serialization: Shounen Sunday
Score: 7.561 (scored by 528 users)
1 indicates a weighted score. Please note that 'Not yet published' titles are excluded.
Ranked: #32062
2 based on the top manga page. Please note that 'R18+' titles are excluded.
Popularity: #4349
Members: 2,378
Favorites: 35


text:
Thailand is one of the most visited countries on the planet, attracting a whopping 35 million travellers every year. Our team is definitely part of that group: we're crazy about the stunning beaches of Koh Samui, gaga for the clifftop villas of Phuket and all about the big city buzz of Bangkok. This time, we're turning our gaze towards Thailand's northern gem – here is our guide to all things Chiang Mai.
Chiang Mai – Orientation
Chiang Mai is the largest city in northern Thailand with a population of some 130,000, though the larger metropolitan area is home to near one million people. The city's history reaches all the way back to the 13th century and Chiang Mai still maintains a significant historic, cultural and religious importance to this day. The city is home to literally hundreds of Buddhist temples and the Old City is still surrounded by a moat, with parts of the ancient city walls still left standing. You don't have to drive far from the city to be surrounded by green rice fields, lush forest and unfiltered sounds of nature. If you ask us, Chiang Mai has it all – the only thing you'll be lacking is beach access.
Things To Do in Chiang Mai
Temple Visits
Chiang Mai and its surrounding hills are home to more than 300 Buddhist temples or "wats" so it's practically impossible to travel to the region without visiting at least one of them.
The most famous and most visited temple is Wat Phra That Doi Suthep located outside the city up a winding mountain road. You need to climb a few hundred steps to reach the top, so this one's definitely not for the faint of heart! Inside the city walls, Wat Phra Singh is a gorgeous structure with dramatic Lanna roofs and a beautifully detailed façade. Another popular option in the Old City is Wat Chedi Luang which dates back to the 14th century.
These three temples are among the most popular spots to visit in Chiang Mai, so don't expect to have the place all to yourself. With literally hundreds of temples to choose from, we also recommend visiting the less-known temples and spending more time in each temple rather than trying to cram all the temples in to a single trip. Temple burnout is a very real thing.
Basic Temple Etiquette
- Dress modestly – no mini shorts, beer brand singlets etc.
- Take off your shoes, hat and sunglasses before entering the temple
- Look for signs in English and/or follow worshippers who aren't monks as parts of the temple are for monks only
- Basic decency goes a long way: don't be loud, don't photograph people without asking permission, don't point or make fun of the Buddha
Chiang Mai has a definite spiritual feel to it and it's little wonder that more than a few yoga schools have sprouted up in the area. Since you're surrounded by ancient temples, vibrant Thai culture and lush nature anywhere you go, it's hard not to get inspired to go "om" while you're in town.
I've personally practiced at the Yoga Tree which is one of the most beloved yoga schools in Chiang Mai. Like so many others, I loved the peaceful vibe this place exudes. The yoga school is located in the heart of the Old City, surrounded by green gardens. Yoga Tree offers classes for all experience levels and they also offer meditation circles, Tibetan bowl meditation and more.
Other highly-rated yoga studios in Chiang Mai include Mahassidha Yoga, Freedom Yoga and Wild Rose Yoga. Not surprisingly, Chiang Mai is also a popular destination for yoga retreats, workshops and yoga teacher trainings.
One of the best things about Chiang Mai are the vibrant markets. This is a great chance to soak up the atmosphere and do some souvenir shopping while also snacking on some local treats.
The most famous market in town is the Night Bazaar, centrally located at the intersection between Tha Pae and Chang Klang Roads. This huge complex offers everything from clothing to DVDs and from boho chic apparel to general souvenirs. The weekly Sunday Market in the Old City is a sprawling affair which stretches from the Tha Pae Gate to Ratchadamnoen Road. The stalls are bursting with local handicrafts and trinkets, with restaurants and food stalls offering a bite to eat in between shopping. Another weekend market is the Wualai Walking Street Market which takes place every Saturday.
Chiang Mai is also home to several markets which focus on food items and fresh produce. Even if you're not planning to cook a full on Thai meal while you're in town, the markets are worth a visit if just for the ambiance and photo ops.
Visiting Thailand without getting at least one massage should be against the law! Chiang Mai offers an endless option of spas and wellness centres with something to suit every budget.
If you're looking for the best deal in town, you can't go wrong with Pranom Health Massage. This is a fantastic budget friendly option located in the heart of the Old City. An excellent one-hour Thai massage is priced at a ridiculously low THB 180 (USD $6).
If on the other hand you're looking for the royal treatment, The Dheva Spa & Wellness Centre is one of your best bets in Chiang Mai. This gorgeous resort is built mimicking an ancient Lanna kingdom with huge gardens and ornately carved pavilions transporting you somewhere that feels beyond space and time. Though definitely on the pricey size – an 80-minute Thai massage will set you back THB 4,200 (USD $129) – Dheva Spa definitely worth it if you're really looking to be pampered.
Explore Nature
Chiang Mai is not just about the temples – the surrounding nature is also worth an adventure or two! One of the most popular excursions in the area is the Flight of the Gibbon, a thrilling zipline course which darts across the jungle and allows guests to spot real live gibbons.
If you're not after a heart pounding adrenaline ride, Chiang Mai's surroundings offer a beautiful setting for jungle treks and white water rafting. You can also easily organise day trips to neighbouring villages – the bohemian village of Pai located up in the mountains is one of the most popular options.
Where to Eat in Chiang Mai
Fine Dining
David's Kitchen
Simply put, David's Kitchen is the best restaurant in Chiang Mai and everyone else can just go home. Voted as the best fine dining restaurant in Chiang Mai, Thailand and all of Asia, it seems this Chiang Mai institution can do no wrong.
The Service 1921
Located at the sumptuous Anantara resort, The Service 1921 was once home to the British consulate and still maintains a grand colonial air to this day. Both the menu and the restaurant have a whimsical British intelligence theme to them. The restaurant serves Thai, Sichuanese and Vietnamese cuisine along with superlative Asian cocktails.
Inspired by the coffee culture of Australia, Ristr8to serves amazing coffee and is perhaps most famed for their ambitious latte art. If you're looking for a serious caffeine hit, this is the place to go. Ristr8to has three locations in Chiang Mai.
Woo Café & Art Gallery
Woo is a beautifully styled café, gallery and lifestyle shop serving Instagrammable lattes and Western-Thai fusion cuisine prepared using fresh local ingredients. Located above the cafe, the art gallery features abstract paintings and sculptures.
Street Food
Khao Kha Moo Chang Phueak
This unassuming food stall shot to fame thanks to a visit from the late, great Anthony Bourdain. Though there are countless stalls in the area surrounding the Chang Phueak gate, you'll know you've found the right one when you spot a Thai lady sporting her signature cowboy hat, wielding a giant cleaver. The stall serves scrumptious Thai pork, street food style.
Rod Yiam
Rod Yiam is routinely ranked as one of the best noodle houses in Chiang Mai. This no-nonsense eatery serves beef noodles in a hearty broth. Frequented by locals, this is a great place to sample some authentic Thai food while also getting a feel of the local way of life, away from the tourist track.
Where to Stay in Chiang Mai
In Chiang Mai, you'll want to stay in a place that actually feels like Thailand. Chiang Mai Luxury Villa achieves just that with classic Lanna style villas set amidst lush tropical gardens framed by the Mae Ping River. The property consists of two villas – Ta Chang Villa with four bedrooms and Spa Villa with five. Each villa features luxurious bedrooms housed in Thai style pavilions arranged around a private swimming pool. The villa staff includes a professional culinary team with a focus on authentic Thai cuisine.
Don't let the name fool you – Howie's amazing luxury pad is unlike any homestay you've ever seen. This sprawling five-bedroom estate is built amidst scenic ponds and offers all-inclusive luxury with an opulent Thai finish. Full-board meals along with airport transfers and stocked minibars are all included in the rates. Though the centre of Chiang Mai is just a short drive away, all these luxury inclusions might just tempt you to stay in instead.


text:
(715) 355-1590
Created by Shelly Lefkoe
- Are you in a power struggle with your children?
- Do you feel drained and exhausted?
- Do you wonder if what you are doing is the best approach to parenting?
- Do you want to raise well adjusted children with high levels of self esteem?
If you answered "Yes" to any or all of these questions, this program is for YOU!
"Parenting the Lefkoe Way" is a 7-part program, condensed into a 3 week class to better accommodate busy schedules. You will learn skills and strategies necessary to raise happy, healthy children. It is designed as a proactive approach to parenting, with an emphasis on awareness and conscious living.
Along with discovering positive parenting skills, you will uncover beliefs that you have that may be causing challenges in your home.
Special Price: $79Bring a companion for an additional $39
Weekly Topics
Meeting 1 - A Revolutionary Approach to Parenting/The Two Most Important Questions
Meeting 2 - Belief Systems and Effective Parenting/Building Self Esteem in Children/The Secrets of Effective Parenting
Meeting 3 - Beliefs That Destroy Families/Beliefs Beyond Your Control
Gather with other parents to gain support, create new strategies, and learn how this very simple way of parenting will change your home environment.
Class is limited to 10 participants.
"When I heard about the parenting class, I was unsure about what that meant. I thought I knew how to parent. I also knew that things did not run smoothly at our house, lots of yelling and complaining. So I went into this not knowing what to expect, but with an open mind. It ended up being so much more than a parenting class. I learned so much about myself and my beliefs. I learned how to calmly deal with situations that in the past, would drive me crazy. I was so excited each week to come home and try some of the techniques that we would learn each night. When I realized how many times they worked and how it didn't take much to do them, I was amazed! It made me feel so good every time I could put a technique into use. I found myself constantly looking for situations to use them. I have used so much of what I've learned in every part of my life, not just with my kids, but also at work. I would encourage anybody with children of any age to take this class!! Lynn does an awesome job of reviewing and answering questions through the whole program. She gives great examples to a lot of different situations. I loved our conversations! Thank you Lynn. This has been life changing for me!"- Sherri, Wausau
"Lynn's class was life changing. Never before have I been so aware of what comes out of my mouth and the effect it has on others. Lynn was able to facilitate an environment of growth and change, and after practicing the techniques, we were able to see almost immediate results. Parents should be required to take this class, as the impact it will have on their children will last a lifetime. Every week left you eager to return to share and learn. Great job Lynn!"- L.D. Wausau
"Thank you for this class. There were times I had to fight back tears because you really made me think about what I was doing as a parent. I wasn't ever hurting my child, but I was treating her as a small adult and not seeing things from her point of view or not really listening/hearing her, on her level. I am so glad I took this class when my child was so young so that I now have the tools going forward."- Wausau
"This has been a life changing experience for me and my family."
"I liked being able to share our experiences using what we learned. I liked hearing Lynn's stories and how she used this method."
"I wish we could have had longer discussion time either before or after the class."
"This class was awesome!"
"I liked what I learned about myself. Loved the enthusiastic leader. It was inspirational!"
"The personal experiences shared I thought were very valuable in the learning process."
"I liked learning how to communicate, then seeing the change in my child."
"I loved being able to share my own challenges and receive input. I loved learning many life-changing ways to think and practical ways to make changes."
"I have seen changes immediately!"
"It was too cheap! You could charge more for this program. It is super awesome!"
"The information and directives were great. You did great, honest, and making it your own program based on Lefkoe's way."
"How could you NOT want this for your children & family?"
"It was very enjoyable."
"I would recommend this program to others."
If you have a group of parents interested in the class, please contact me and we will set up a time that is convenient for your group OR call if you alone are interested. I may be in the process of forming a group and we would love to invite you to join us!
Please call 715-355-1590 or email us
Although this program is designed to be proactive and preventative, all parents/grandparents will greatly benefit from its content.
It is never too late to change beliefs!
Vision to SOAR Life Coaching


text:
...our model shows that alcohol taxes may be counter-productive in controlling the emergence of EDSS [Excessive Drinking in Social Situations] as a social norm.However, don't rush to cancel alcohol taxes just yet. These results are based primarily on a theoretical model, and while the authors present some circumstantial evidence to support it (a survey of Norwegian students, and noting that states in the US with more historical Scandinavian immigrants are more likely to demonstrate a binge drinking culture), the conclusions are largely speculative.
There is a long chain of results required to link genetic variations with binge drinking. First, the authors note that there are studies that link shyness to genetic characteristics like blue eyes and tall ectomorphic bodies (which are typical Scandinavian traits). Second, shy people can use excessive drinking as a coping strategy in social situations. Third:
...given the first two steps, a larger fraction of the population in northern Europe should have a greater genetic disposition for EDSS...
Fourth, if the fraction of the population with EDSS genetic disposition is sufficiently large, social reinforcement mechanisms could come into play... If EDSS is atypical, it is considered anti-social. If EDSS is relatively common, it has a "legitimizing" effect and EDSS becomes acceptable.
Fifth, if steps 1-4 hold, we should observe a larger fraction of the population that engages in EDSS in northern Europe than in southern Europe.It is a nice theoretical model, but given the number of steps required to link genetic variation with binge drinking, file this as speculative at best. At least, until there is some genuine empirical support for it. I find it hard to get past the counter-examples of countries like New Zealand, Australia, and the UK, where blue eyes are not common and yet binge drinking is.


text: Out of Lyme & Divine 9 Adaptogen Combo Set Sale! from $89.50 Qty. Add to Cart This item is currently on backorder but you can still purchase it now and we'll ship as soon as more become available. Out of Lyme in combination with Divine 9 Adaptogen yields better results. We recommend taking Out of Lyme and Out of Lyme PLUS sets rotationally. Do not take Out of Lyme and Out of Lyme PLUS at the same time.


text:
1850 Priority Way, 40299 Louisville, United States Show on the map
- WiFi
- Car park
- Air conditioning
- Pets admitted
- Accessible
- Family
- Gym
- Business
Excellent location
Choose your room
Whether travelling for business or leisure, the hotel's friendly staff will ensure that you have a memorable vacation. This is a pet-friendly hotel. The spacious guestrooms provide comfortable stay. Guests can enjoy complimentary Continental breakfast during their stay at this hotel.
Check-in and check-out
Check-in starts at: 15:00
Check-out ends at: 11:00
Useful information:Other names for Hotel Sleep Inn Louisville:
Sleep Inn East


text:
School conjuration (creation) [metal]; Level antipaladin 4, cleric 5, inquisitor 5, magus 5, saboteur 5, vanguard 5, witch 5
Casting Time 1 standard action
Components V, S
Range close (25 ft. + 5 ft./2 levels)
Target one creature
Duration 1 round/level (D)
Saving Throw Reflex partial; Spell Resistance yes
A groaning, creaking sound of stressed metal echoes around you as you finish casting this spell. Large walls of a coffin-shaped container appear on either side of a creature, their interiors covered in spikes. The two halves slam together, trapping the creature inside.
Iron maiden creates a large, magically-reinforced iron maiden to trap a single creature. The iron maiden is appropriately sized for the targeted creature. If the creature fails its saving throw, it is completely encased by the iron maiden. It is considered pinned by the iron maiden and can take no actions except trying to break free. It gains total cover from those outside of the iron maiden, and those outside have total cover from it. Each round, the creature takes 3d8 piercing damage from the spikes within the iron maiden. The spikes are considered magical for the purpose of overcoming damage reduction. No light penetrates the iron maiden.
A creature that succeeds on its saving throw is only partially caught by the spell; it is considered grappled by the iron maiden and cannot move from its space, but can otherwise act normally. It also takes half the damage it would normally take from the iron maiden each round.Whether the creature failed or succeeded on its saving throw, it can attempt to break out of the iron maiden in a few ways. The iron maiden has hardness 10 and 120 hit points and can be destroyed by enough damage. It can also be broken in a single attack with a DC 30 Strength check. A creature that is grappled (but not pinned) by the iron maiden can attempt an Escape Artist check or a combat maneuver check to break free (DC 15 + your caster level). A creature does not add its size bonus to its maneuver check to escape in this manner (as the iron maiden is always large enough to hold it). A creature that is pinned by the spell can only attempt to break it with a Strength check; it is otherwise unable to act. If the target escapes the iron maiden, or if it is destroyed, the spell ends.
Path of Iron, © 2015, Ascension Games, LLC; Author Christopher Moore


text:
P.F Candle Co.
NO. 19 | PATCHOULI SWEETGRASS Candle 3.5oz
Regular price £10.50 Sale price
NO. 19 | PATCHOULI SWEETGRASS Candle by P.F Candle Co.
- The modern take on the classic patchouli.
- This scent pairs vibrant sweetgrass with creamy sandalwood and smoky cedar.
- Calming yet bright and unexpected
- MINI Soy candle hand-poured into apothecary-inspired amber jars.
- Glass Jar, Brass Lid with Cotton Wick
- Burn Time 20-25 hrs.
- Size: 3.5oz | 2.25" x 2.75"
- Made in LA. CA Studio
- 100% Natural Soya Wax
- Phthalate-Pree Fragrance oil
- Not tested on animals
- Vegan
Please Note
- Keep the candle away from flammable objects and never leave them unattended!
- Always trim your wick to 1/4" before each lighting.


text:
Move a WordPress Site to a New Host
Post is under construction. Check back very soon! Try the 'Website Building' menu above one more time. About Steve. A working guitarist, guitar designer, songwriter, worship leader and online entrepreneur, I have discovered that the key to online success (and success is more than money) is simply to focus on helping people be successful.


text:
The IL-2/IL-2R interaction is important for development and peripheral homeostasis of T regulatory (Treg) cells. IL-2- and IL-2R-deficient mice are not completely devoid of Foxp3+ cells, but rather lack population of mature CD4+CD25+Foxp3high Treg cells and contain few immature CD4+CD25−Foxp3low T cells. Interestingly, common γ chain (γc) knockout mice have been shown to have a near complete absence of Foxp3+ Treg cells, including the immature CD25−Foxp3low subset. Therefore, other γc-cytokine(s) must be critically important during thymic development of CD4+CD25+Foxp3+ Treg cells apart from the IL-2. The present study was undertaken to determine whether the γc-cytokines IL-7 or IL-15 normally contribute to expression of Foxp3 and Treg cell production. These studies revealed that mice double deficient in IL-2Rβ and IL-7Rα contained a striking lack in the CD4+Foxp3+ population and the Treg cell defect recapitulated the γc knockout mice. In the absence of IL-7R signaling, IL-15/IL-15R interaction is dispensable for the production of CD4+CD25+Foxp3+ Treg cells, indicating that normal thymic Treg cell production likely depends on signaling through both IL-2 and IL-7 receptors. Selective thymic reconstitution of IL-2Rβ in mice double deficient in IL-2Rβ and IL-7Rα established that IL-2Rβ is dominant and sufficient to restore production of Treg cells. Furthermore, the survival of peripheral CD4+Foxp3low cells in IL-2Rβ−/− mice appears to depend upon IL-7R signaling. Collectively, these data indicate that IL-7R signaling contributes to Treg cell development and peripheral homeostasis.
Naturally occurring CD4+CD25+Foxp3+ regulatory T cells (Treg)3 develop in the thymus as a fully functional distinct subset of CD4+ T cells that migrate to the periphery to actively suppress autoreactive T cells that escaped thymic negative selection. Along with Foxp3, expression of IL-2R represents one of the better-characterized molecules of Treg cells. Furthermore, the IL-2R is functionally important for Treg cells as IL-2- and IL-2R-deficient mice develop rapid lethal autoimmunity due to impaired production of these cells (1). The IL-2/IL-2R interaction, primarily through STAT5, contributes to thymic Treg cell development and their homeostasis in peripheral immune tissues (2, 3, 4, 5). An important consequence of the IL-2/IL-2R interaction in Treg cells is to maintain optimal expression of Foxp3 and CD25. The Foxp3 gene contains consensus-binding sequences for STAT5 that are required for transcription, indicating an important role STAT5 in Foxp3 expression (6, 7). Furthermore, IL-2 was shown to regulate Foxp3 expression in both mouse and human CD4+CD25+ regulatory cells through STAT5-dependent signals revealing the critical link between IL-2 and STAT5 signaling in the maintenance of Foxp3-expressing regulatory cells (6, 7, 8). Mice deficient in IL-2Rβ, IL-2Rα, or IL-2 are not devoid of Foxp3+-expressing CD4 T cells, but rather contains a reduced number of immature CD4+CD25−Foxp3low T cells. Although these immature Foxp3lowCD4 T cells in IL-2−/− mice exhibit some suppressor function in vitro, they do not suppress autoreactive T cells in vivo and are found at very low numbers in the periphery of IL-2Rβ−/− mice that were rendered autoimmune-free through adoptive transfer of a low number of wild-type (WT) Treg cells (2, 9). Furthermore, when endogenous Foxp3 is attenuated in Treg cells, mice succumb to lethal autoimmunity (10). These results taken together are consistent with an intrinsic defect in IL-2/IL-2R-deficient Foxp3lowCD4+ T cells.
Induction of Foxp3 is a hallmark of commitment to Treg cell lineage (11). Therefore, the signals leading to Foxp3 expression in the thymus represent key steps in development of this T cell subset. In this regard, common γ chain (γc)−/− mice exhibit a much more striking defect in the development of Foxp3+ cells than IL-2- or IL-2R-deficient mice, with a near complete absence of these cells in both the thymus and periphery (3, 5). Therefore, another γc-cytokine along with IL-2 functions during thymic development of Treg cells. Because the development of natural Treg cells depends on signaling through STAT5 (3, 6, 12, 13), in the current study we assessed whether IL-7 or IL-15, which like IL-2 also activates STAT5 (14, 15), contributes to expression of Foxp3 and Treg cell production. By evaluating mice that do not respond to IL-2, we uncovered a role for IL-7R signaling for Treg cell development and peripheral homeostasis that usually is dominated by IL-2.
Materials and Methods
C57BL/6, B6129SF2/S, IL-15Rα−/−, and γc−/− mice were obtained from The Jackson Laboratory. The thymic-targeted transgenic WT IL-2Rβ expressed in IL-2Rβ−/− mice (designated 2RβTG in this study; thymic transgene (TG)) and the thymic-targeted WT IL-7Rα expressed in IL-7Rα−/− mice (designated 7RαTG in this study) on the C57BL/6 genetic background have been previously described (16, 17). These mice, along with IL-7Rα−/− mice, were maintained in the breeding colony at the University of Miami. Double deficient IL-7 and IL-15 (double knockout (DKO)IL-7/IL-15) mice on the C57BL/6 genetic background have been previously described (18) and were bred and maintained at The Scripps Research Institute. Thymic transgenic mice expressing WT IL-2Rβ and/or IL-7Rα on the double deficient IL-2Rβ−/− and IL-7Rα−/− (DKO2Rβ/7Rα) were generated by intercrossing 2RβTG and 7RαTG. The progeny were then crossed, resulting in 2RβTG 7RαKO and IL-2Rβ+/−7RαTG mice that were used as breeding pairs to generate and maintain the following lines of mice: DKO2Rβ/7Rα, 2RβTG7RαTG DKO2Rβ/7Rα, 2RβTGDKO2Rβ/7Rα, and 7RαTGDKO2Rβ/7Rα. Protocols were approved by the Institutional Animal and Use Committee at the University of Miami and at The Scripps Research Institute.
Experimental animals
C57BL/6, DKO2Rβ/7Rα, 2RβTG7RαTGDKO2Rβ/7Rα, 2RβTGDKO2Rβ/7Rα, and 7RαTGDKO2Rβ/7Rα mice were sacrificed 3–15 wk of age. The B6126SF2/S and IL-15Rα−/− or γc−/− mice were sacrificed at 7–12 or 6 wk of age, respectively. Inguinal, axillary, brachial, and cervical lymph nodes were collected at the time of sacrifice along with the spleen and thymus. Tissues were made into single-cell suspensions and subjected to FACS analysis.
FACS analysis
For four-color analysis, cells were incubated for 20 min at 4°C with Pacific Blue-CD4, PerCP-CD8, and PE-Cy7-CD25 followed by two washes with HBSS containing 0.2% BSA and 150 mM NaN319) using a Becton Dickinson LSRII and Diva software. The total number of events collected for analysis was between 500,000 and 1,000,000.
Mice doubly deficient in IL-2Rβ and IL-7Rα lack CD4+Foxp3+ Treg cells
Past work revealed that IL-2Rβ−/− mice contain CD4+Foxp3+ T cells, but when compared with WT mice, the number of these cells was reduced 2-fold in the thymus and 4-fold in peripheral immune tissues, with a 2-fold lower level of intracellular Foxp3 protein [Ref. 2 and see Fig. 4)]. Initially, we investigated whether other IL-2-independent STAT5-activating γc-dependent cytokines, i.e., IL-7 and IL-15, individually contribute to the development and homeostasis of natural Treg cells. The IL-15Rα−/− mice have largely normal production and maintenance of CD4+CD25+Foxp3high Treg cells (Fig. 1⇓A, C, and D). Although not significantly different, we observed that the thymus of IL-15Rα−/− mice contained a somewhat lower number of CD4+Foxp3+ cells. The spleen contained a percentage and number of CD4+CD25+Foxp3+ T cells comparable to littermate control B6129SF2/S mice (Fig. 1⇓A, C and D). IL-15Rα−/− mice did not exhibit any signs of autoimmunity associated with IL-2/IL-2R-deficiency (data not shown).
IL-7Rα-deficient mice have a severe block in T cell development, such that thymic cellularity is reduced 10- to 20-fold, but those cells present usually progress through all major thymic stages (Fig. 2⇓ and Table I⇓). Reduced thymic number is largely due to a developmental arrested at the pre-T cell stage (20), leading to a peripheral immune compartment that is severely lymphopenic due to low number of T and B cells. Sometimes the arrest at the pre-T cell stage in IL-7Rα−/− mice prevented further T cell development and these mice (three of eight) were excluded from this analysis. Nevertheless, IL-7Rα−/− mice have a normal fraction of CD4+CD25+Foxp3+ Treg in both the thymus and spleen when compared with WT B6 mice, although IL-7Rα−/− mice contain a higher fraction of CD4+CD25+Foxp3− thymocytes (Fig. 1⇑, B and C). Despite the severely reduced thymic and splenic cellularity, the number of CD4+Foxp3+ thymocytes or splenocytes was largely comparable to B6 mice when normalized to 1 × 106 cells/tissue (Fig. 1⇑D, left bottom panel). Although severe lymphopenia has been associated with immune dysregulation and autoimmune diseases (21, 22, 23), IL-7Rα−/− mice were overtly healthy. In total, these findings and similar analysis of IL-7−/−, IL-15−/−, IL-7Rα−/−, and IL-15Rα−/− mice (3, 24, 25) indicate that thymic development and peripheral homeostasis of CD4+Foxp3+ Treg cells do not require either of these cytokines when there is IL-2R signaling.
The presence of immature CD4+Foxp3low cells in IL-2Rβ−/− mice, i.e., CD4+ T cells with ∼2- to 4-fold lower level of Foxp3 based on the mean fluorescence intensity. [Fig. 3⇓B and Ref. 2 ], indicates that development of these cells is independent of both IL-2 and IL-15, as signaling by these cytokines depends upon IL-2Rβ. Furthermore, in WT B6 mice, a small but detectable population of thymic CD4+Foxp3+ Treg cells expresses low levels of IL-7Rα (CD127), and the majority of periphery Treg cells express similarly low levels of IL-7Rα (Fig. 3⇓A). Detection of such low levels of IL-7Rα is specific as IL-7Rα staining on WT cells was greater than that observed on control cells from IL-7Rα−/− mice (Fig. 3⇓A). The present finding also confirms previously published reports on mouse Treg cells and is in line with the phenotype of human Treg cells in the peripheral blood of both healthy and type 1 diabetic patients (26, 27). To directly test whether these IL-2/IL-15-independent Foxp3-expressing cells required IL-7, existing knockout animals were crossed to obtain mice deficient in both IL-2Rβ and IL-7Rα, i.e., DKO2Rβ/7Rα mice. As expected, thymic and splenic cellularity in DKO2Rβ/7Rα mice was dramatically reduced (Table I⇑). Despite this reduced cellularity, thymic development was largely normal in these mice with progression through all major thymic subsets (Table I⇑ and Fig. 2⇑). The peripheral compartment in DKO2Rβ/7Rα mice contained predominantly CD4 T cells (Table I⇑ and Fig. 2⇑), probably, because CD8 T cells survive poorly with a combined loss of IL-7 and IL-15. Importantly, DKO2Rβ/7Rα mice contained a striking defect in the CD4+Foxp3+ cells with a near complete absence in the thymus and spleen (Fig. 3⇓, B–D). Based on their relative abundance, WT B6 mice contained 6- and 14-fold more CD4+Foxp3+ thymocytes and splenocytes, respectively, than the DKO2Rβ/7Rα mice (Fig. 3⇓, C and D). This defect in CD4+Foxp3+ cells in DKO2Rβ/7Rα mice was much more striking than found in IL-2Rβ−/− mice [Fig. 3⇓B and Ref. 2 ]. The number of CD4+Foxp3+ cells in the thymus of DKO2Rβ/7Rα was slightly lower and not statistically significant when compared with that in γc−/− mice (Fig. 3⇓, B and C). Although DKO2Rβ/7Rα and γc−/− mice do not exhibit severe autoimmunity due to impaired T cell development, both types of mice contained peripheral T cells that consisted of reduced CD8+ T cells, but CD4+ T cells with an activated phenotype (Table I⇑ and data not shown). Collectively, these data indicate that the lack of Foxp3+ T cells in γc−/− mice is due to impaired signaling through both IL-2Rβ and IL-7Rα-chains.
IL-2 and IL-7 are essential for CD4+CD25+Foxp3+ Treg cell production
Because IL-2Rβ-chain is shared by both IL-2 and IL-15 receptors, the defect in CD4+Foxp3+ cells in DKO2Rβ/7Rα and γc−/− mice might reflect the absence of IL-2, IL-7, and IL-15 or a loss in IL-7 and either IL-2 or IL-15. This possibility was explored by examining whether the thymus and lymph nodes from mice doubly deficient in IL-7 and IL-15, i.e., DKOIL-7/IL-15 mice, resembled that from DKO2Rβ/7Rα and γc−/− mice. However, even though thymic cellularity was reduced, DKOIL-7/IL-15 mice have a proportion of thymic CD4+CD25+Foxp3+ T cells comparable to WT B6 mice, while the periphery contains a higher fraction of CD4+CD25+Foxp3+ Treg cells when compared with WT mice (Fig. 4⇓, A and B). Thus, in the absence of IL-7R signaling, IL-15 is dispensable for production and maintenance of CD4+Foxp3+ T cells. Given that IL-2- and IL-2R-deficient mice have comparable number of immature CD4+Foxp3+ T cells (2, 5) and that the IL-15/IL-15R interaction is not essential for Treg cell thymic development or peripheral homeostasis [this report and Ref. 3 ], these data suggest that IL-2 is sufficient to drive full expression of Foxp3 and CD25 on Treg cells.
Thymic-targeted IL-2Rβ expression is sufficient to restores the production of CD4+CD25highFoxp3high Treg cells in DKO2Rβ/7Rα mice
To further explore the role of IL-7 and IL-2 signaling in Treg cell development, we examined the production of CD4+Foxp3+ T cells in the thymus of DKO2Rβ/7Rα mice that were reconstituted singly with thymic-targeted transgenic IL-2Rβ or IL-7Rα and doubly with both transgenes, using previously developed transgenic mice. Thymic-directed IL-2Rβ (2RβTG) has been shown to restore Treg production and prevent autoimmunity when expressed in IL-2Rβ−/− mice (2, 9, 16). Thymic-directed IL-7Rα (7RαTG) expression has been shown to promote mainstream thymic T cell development when expressed in IL-7Rα−/− mice (17, 28), while peripheral T cells do not obviously express or functionally respond to IL-7 (29).
In either thymic transgenic DKO2Rβ/7Rα mice, T cell development was largely normal with only minimal alterations in the major thymic subsets (Table I⇑ and Fig. 2⇑). Previous work from our laboratory demonstrated that 2RβTG in IL-2Rβ−/− mice was detected at high levels in double positive (DP) CD4+CD8+Foxp3-expressing thymocytes with very little detectable CD122 expression in the single positive (SP) CD4+CD8− Foxp3-expressing cells (2). The 7RαTG expression alone or in combination with 2RβTG in DKO2Rβ/7Rα mice (7RαTGDKO2Rβ/7Rα or 2RβTG7RαTGDKO2Rβ/7Rα, respectively) also resulted in low CD127 expression being largely restricted to DP Foxp3+ thymocytes, with essentially none on SP CD4+CD8− Foxp3-expressing cells (Fig. 5⇓A). With respect to Treg cell development, either 7RαTG or 2RβTG thymic-directed transgene led to the production of Foxp3-expressing CD4+ T cells (Fig. 5⇓, B and C). With respect to Treg cell thymic development, 7RαTGDKO2Rβ/7Rα mice contained a population of immature CD4+CD25−Foxp3low thymocytes as seen in IL-2Rβ−/− mice, with a decreased frequency of Foxp3-expressing CD4+CD8− SP thymocytes (Fig. 5⇓B, C, and D). Furthermore, these immature Foxp3low T cells in 7RαTGDKO2Rβ/7Rα mice did not suppress autoimmunity (data not shown). These data indicate that IL-7R signaling is not sufficient to fully support production of mature CD4+CD25+Foxp3high Treg cells. In contrast, expression of the 2RβTG in DKO2Rβ/7Rα mice (2RβTGDKO2Rβ/7Rα) or both transgenes in DKO2Rβ/7Rα mice (2RβTG7RαTGDKO2Rβ/7Rα) reconstituted a normal compartment of mature CD4+CD25+Foxp3high thymocytes (Fig. 5⇓B, C, and D) and these mice did not shown signs of autoimmunity (data not shown). Furthermore, the expression of both 2RβTG and 7RαTG in the DKO2Rβ/7Rα mice resulted in ∼2.6-fold more Foxp3-expressing SP CD4 thymocytes. This increase is likely due to the broader expression on IL-2Rβ and IL-7Rα in the thymus of these DKO mice, which favored a greater production of these cells. Importantly, the data from 2RβTGDKO2Rβ/7Rα mice indicate that IL-2R signaling is sufficient to restore Treg cell development and function leading to a normal population of mature CD4+CD25+Foxp3high Treg cells (2, 9, 30).
A role for IL-7 in peripheral Treg cell homeostasis
Past work showed that IL-2Rβ is expressed at nearly undetectable levels by peripheral CD4+Foxp3+ Treg cells within IL-2Rβ−/− mice containing the thymic-directed 2RβTG (2). This minimal IL-2Rβ did not support IL-2-dependent Treg cell activity when directly evaluated in several assays in vivo and in vitro, but resulted in weak transient IL-2-dependent STAT5 activation. Thus, peripheral Treg cell function and homeostasis can be maintained in the periphery in an IL-2-independent fashion or with minimal IL-2-dependent STAT5 activation. These peripheral Treg cells with impaired IL-2Rβ signaling exhibited diminished proliferation and turnover. Thus, these homeostatic properties more closely resembled naive conventional CD4 T cells, which are known to depend in part on IL-7 (20). Thus, we investigated whether IL-7R signaling might account for peripheral Treg cells in 2RβTG IL-2Rβ−/− mice.
In a manner similar to 2RβTG, IL-7R expression is not detected on peripheral T cells within IL-7Rα−/− mice containing the thymic-directed 7RαTG and peripheral T cells from these mice are functionally impaired when stimulated with IL-7 in vitro and in vivo, but IL-7 also induced weak and transient STAT5 activation (29). Therefore, we examined whether lowering IL-7R signaling affected the survival of IL-2R-defective peripheral Treg cells by enumerating the relative abundance of CD4+CD25+Foxp3high T cells in the spleens of 2RβTG7RαTGDKO2Rβ/7Rα and 2RβTGDKO2Rβ/7Rα mice. The periphery of these transgenic DKO2Rβ/7Rα mice was largely normal except that these mice contained a higher percentage of CD4 T cells (Table I⇑ and Fig. 2⇑), which may be due to the broader activity of these transgenes in the thymus favoring greater production of these cells. In both mice, the proportion of Treg cells was essentially identical with that found within the spleen of WT B6 mice (Fig. 6⇓, A and B). Thus, these data suggest peripheral Treg cell homeostasis can be independent of IL-2 or IL-7 or that the remaining weak STAT5 activation is sufficient in the context of TCR or other signals.
Immature CD4+CD25−Foxp3low cells are readily found in IL-2Rβ−/− mice and develop due to thymic IL-7R signaling, as evident through evaluation of 7RαTGDKO2Rβ/7Rα mice (Fig. 5⇑). Thus, we also examined the periphery of 7RαTGDKO2Rβ/7Rα mice for CD4+Foxp3+ T cells. In marked contrast to 2RβTG7RαTGDKO2Rβ/7Rα mice, the spleen of 7RαTGDKO2Rβ/7Rα contained very few CD4+Foxp3+ cells, but rather contained a relatively small population of immature CD4+CD25−Foxp3low T cells (Fig. 6⇑A). Based on relative abundance, the spleen of 7RαTGDKO2Rβ/7Rα mice have 11- and 2.4-fold fewer cells in the spleen than WT and IL-2Rβ−/− mice, respectively (Fig. 6⇑C). This decrease was also similar in the lymph nodes (Fig. 6⇑C). Unlike peripheral immature CD4+Foxp3low T cells from IL-2Rβ−/− mice, which can respond to IL-7, peripheral cells in 7RαTGDKO2Rβ/7Rα mice are genetically non-responsive to IL-2 and IL-15 and only minimally responsive to IL-7. Thus, the lower fraction of peripheral CD4+Foxp3low T cells when compared with CD4+Foxp3low T cells in IL-2Rβ−/− mice (2) suggests that IL-7/IL-7R may contribute to survival of the peripheral immature Foxp3low T cells.
We have described two roles for IL-7R for naturally occurring CD4+Foxp3+ Treg cells. One function of IL-7R is during thymic development that was uncovered by analyzing DKO2Rβ/7Rα mice, which recapitulated the phenotype of γc−/− mice. This study revealed a relatively normal production of Treg cells by DKOIL-7/IL-15 mice, which strongly suggests that IL-15R signaling is dispensable in the absence of IL-7R and that IL-7 and IL-2 are the only γc-dependent cytokines that normally function during Treg cell development. Furthermore, expression of either 2RβTG or 7RαTG in DKO2Rβ/7Rα mice established that IL-2R signaling is dominant and sufficient for development of Treg cells and that the CD4+CD25−Foxp3low thymic phenotype is a consequence of IL-7R signaling, accounting for these cells in IL-2Rβ−/− mice. We currently do not know how IL-7 promotes development of these Foxp3low thymocytes. Normal Treg cell development was rescued in IL-2Rβ−/− mice after expression of a transgenic chimeric receptor linking IL-2 binding to IL-7R signaling but not reciprocally after linking IL-7 binding to IL-2R signaling (28). This finding suggests IL-7 is not available in the niche where IL-2 normally functions. Therefore, we favor the notion that IL-7 promotes Foxp3 expression at point in thymic development distinct from where IL-2 functions. We speculate that the IL-7R signal is delivered early, perhaps during pro/pre-T cell development where IL-7R normally functions (Fig. 7⇓), only to be revealed later by another developmental signal, because IL-7R-dependent Foxplow cells in IL-2Rβ−/− mice are first readily found at the CD4+CD8+ stage of thymic development (2). Furthermore, the data from the 2RβTGDKO2Rβ/7Rα mice along with our past work support that the essential role for IL-2R resides later during the DP and/or SP stage (Fig. 7⇓).
IL-7 and thymic stromal lymphopoietin (TSLP) both use IL-7Rα as a subunit and activate the transcription factor STAT5 (31). Therefore, the defect in IL-7Rα potentially reflects impairment due to both of these cytokines. For example, IL-7Rα deficiency results in greater block in T and B cells compartments compared with IL-7 knockout mice. Furthermore, mice deficient in both γc and TSLP receptors have a greater lymphoid defect than γc-deficient mice (32). In regards to Treg development, TSLP has been shown to play a role in the differentiation and maturation of Foxp3-expressing cells in the human and murine thymus (31, 33, 34). However, this function of TSLP is indirect in that it acts to condition dendritic cells to express CD80 and CD86, which in turn contributes to the maturation of Treg cells (34). Thus, the detection of normal proportion of mature Treg cells in IL-7Rα−/− mice indicates that the activity of TSLP is not essential for Treg cell production. Furthermore, the γc-chain is a subunit of the IL-7R and not the TSLPR, yet both DKO2Rβ/7Rα and γc-deficient mice do not produce Treg cells. This loss in Treg cells is consistent with IL-7, rather than TSLP, as a critical cytokine along with IL-2 for the normal development of Treg cells. Nevertheless, we cannot completely exclude the possible contribution by TSLP in promoting Treg cell development.
The absence of either IL-15 or IL-15Rα alone does not result in a major defect in Treg cell development [Ref. 3 and this study]. We and others (2, 5) have shown that mice deficient in IL-2, IL-2Rα, or IL-2Rβ have a similar 2-fold reduction in thymic Foxp3-expressing CD4+ T cells. These findings, therefore, support the notion that this reduction in Foxp3+ thymocytes is accounted for by the lack of IL-2/IL-2R function. However, Burchill et al. (3) have suggested a role for IL-15, which also depends upon IL-2Rβ for signal transduction, during Treg cell development because a greater reduction in CD4+Foxp3+ thymic cells was noted in IL-2Rβ−/− mice when compared with IL-2−/− mice. We do not know the reason for these differences, but the absence of both IL-2Rβ and IL-7Rα leads to an essentially complete absence of thymic Foxp3-expressing thymocytes, which is much more striking than the relatively low proportion of Foxp3+ cells detected in IL-2Rβ−/− mice by Burchill and colleagues. Furthermore, the presence of CD4+Foxp3+ T cells in mice deficient in both IL-7 and IL-15 is consistent with no critical role for IL-15 in Treg cell development. Overall, these findings support a model where IL-2 and IL-7 are the main γc-dependent cytokines that normally function during thymic development of Treg cells.
The other function of IL-7Rα may be to promote the survival of peripheral Foxp3+ T cells, which was uncovered only after analysis of the periphery of 7RαTGDKO2Rβ/7Rα mice. In this situation, only immature CD4+CD25−Foxp3low T cells develop due to thymic IL-7Rα expression. The peripheral Foxp3low cells do not respond to IL-2 but generate a weak IL-7 signal through minimal expression of transgenic IL-7R and cannot prevent autoimmunity. These Foxp3low T cells behave identically to the immature CD4+Foxp3low cells in IL-2Rβ−/− mice, which express WT endogenous IL-7R, except their prevalence in the periphery was much lower, raising a potential role for IL-7R in their survival. However, the normal frequency of peripheral Treg cells in IL-7Rα−/− mice is consistent with IL-2 dominantly controlling peripheral Treg cell homeostasis. Quite remarkably, functional peripheral Treg cells with impaired IL-2R and IL-7R signaling were found at a normal frequency in 2RβTG7RαTGDKO2Rβ/7Rα mice. This finding raises the possibility that the increased, albeit it minimal, STAT5 activation through 2RβTG on peripheral Treg cells is sufficient for Treg homeostasis. However, besides this difference, an important distinction between 7RαTGDKO2Rβ/7Rαand 2RβTG7RαTGDKO2Rβ/7Rα mice is that in only the latter mice is an IL-2 signal given leading to production of CD4+CD25+Foxphigh Treg cells that control autoimmunity. Whether this productive developmental signal is favorable for peripheral homeostasis independent of IL-2 and IL-7 remains to be determined.
These data, our past work, and that of others support a dominant role for IL-2 during thymic Treg cell development and peripheral homeostasis (2, 4, 5). Thus, what might be the biological relevance of IL-7 in regulating Treg cells? One possibility is to lower the requirement for IL-2R signaling. This might be particularly relevant during thymic development if the IL-7R-dependent Foxp3low stage precedes the IL-2R-dependent stage as immature Foxp3low thymocytes should require less IL-2 signaling to drive normal Foxp3 levels than cells that have not yet expressed Foxp3. As IL-2 producing cells are limited within the thymus (35), increasing signal sensitivity to IL-2 may assure optimal number of Treg cell over a long time period. Another possibility is that IL-2 production by conventional T cells in the periphery is very transient and Ag-dependent (36). Thus, stromal cell-derived IL-7 might aid Treg cell homeostasis at times when IL-2 is unavailable. Although Treg cell production occurs in the experimental setting of IL-7 (24) or IL-7R-deficiency [this study and Ref. 3 ], impaired T cell development makes autoimmunity less likely. In the real world of diminishing thymic output with age and infection, IL-7 may be a more important factor preventing autoimmune disease at the level of Treg cells. Our study suggests that this may indeed be an important line of future investigation as polymorphism in the IL-7Rα gene, that likely lead to reduced IL-7R signaling, is an autoimmune susceptibility gene for multiple sclerosis and type1 diabetes (37, 38, 39, 40).
We thank Linjian Zhu for technical assistance.
The authors have no financial conflict of interest.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
↵1 This work was supported by National Institutes of Health R01 CA45957 and AI055815 and Juvenile Diabetes Research Foundation International 4-2004-361, Juvenile Diabetes Research Foundation Center at the University of Miami-Diabetes Research Institute.
↵2 Address correspondence and reprint requests to Dr. Allison L. Bayer, Miller School of Medicine University of Miami, Departments of Microbiology and Immunology and Diabetes Research Institute, 1450 Northwest 10th Avenue, Miami, FL 33136. E-mail address: abayer{at}med.miami.edu
↵3 Abbreviations used in this paper: Treg, T regulatory cell; DKO, double knockout; SP, single positive; DP, double positive; TG, thymic transgene; TSLP, thymic stromal lymphopoietin; WT, wild type; γc, common γ chain.
- Received December 17, 2007.
- Accepted May 2, 2008.
- Copyright © 2008 by The American Association of Immunologists


text:
Here are twenty recently-released tracks I've been grooving to of late. Let me know if you have a favorite I should check out.
Tag Archives: Charli XCX
Cool Newish Music (9-28-19)
Filed under Uncategorized
Cool Newish Music
Here are twenty recently-released tracks I've been grooving to of late. Let me know if you have a favorite I should check out.
Click here to like Tunes du Jour on Facebook.
Follow me on Twitter.
Follow me on Instagram.
Filed under Uncategorized
My Top 99 Songs Of 2018
I listened to a lot of music. I crunched the numbers. The result? My top 99 songs of 2018. Here they be, followed by a Spotify playlist:
1. Make Me Feel – Janelle Monae
2. Ric Flair Drip – Offset & Metro Boomin
3. Nice for What – Drake
4. Alone – Halsey featuring Big Sean & Stefflon Don
5. Where Angels Fear to Tread – Disclosure
6. Mr. Tillman – Father John Misty
7. Thunderclouds – LSD (Labrinth, Sia, Diplo)
8. IDGAF – Dua Lipa
9. This Is America – Childish Gambino
10. Finesse – Bruno Mars featuring Cardi B
11. Stir Fry – Migos
12. LOVE. – Kendrick Lamar featuring Zacari
13. Missing U – Robyn
14. Bodak Yellow – Cardi B
15. The Weekend – SZA vs Calvin Harris
16. Mona Lisa – Lil Wayne featuring Kendrick Lamar
17. Boys – Lizzo
18. Lemon – NERD featuring Rihanna
19. Honey – Robyn
20. Boo'd Up – Ella Mai
21. Now – Miguel
22. High Horse – Kacey Musgraves
23. Apeshit – The Carters
24. Tints – Anderson .Paak feat. Kendrick Lamar
25. 5 Dollars – Christine and the Queens
26. Nameless, Faceless – Courtney Barnett
27. When I'm with Him – Empress Of
28. Two Slow Dancers – Mitski
29. Nobody – Mitski
30. Got My Name Changed Back – Pistol Annies
32. I Like It – Cardi B featuring Bad Bunny and J Balvin
33. I Might Need Security – Chance the Rapper
34. Microfiche – Open Mike Eagle
35. If You Know You Know – Pusha T
36. All the Stars – Kendrick Lamar with SZA
37. Mr. Jukebox – Joshua Hedley
38. Crayons – CupcakKe
39. Washing Machine Heart – Mitski
40. Pynk – Janelle Monae featuring Grimes
41. Duck Duck Goose – CupcakKe
42. Bloom – Troye Sivan
43. He's Got His Mother's Hips – John Grant
44. HEAVEN – The Blaze
45. Day I Die – The National
46. Fast Slow Disco – St. Vincent
47. Kong – Neneh Cherry
48. Sangria Wine – Pharrell Williams and Camila Cabello
49. Look Alive – BlocBoy JB featuring Drake
50. Yes Indeed – Lil Baby featuring Drake
52. Need a Little Time – Courtney Barnett
53. You Should See Me in a Crown – Billie Eilish
54. Bardier Cardi – Cardi B featuring 21 Savage
56. Bad Bad News – Leon Bridges
57. Fireworks – First Aid Kit
58. Party for One – Carly Rae Jepsen
59. New Rules – Dua Lipa
60. I'll Never Love Again – Lady Gaga
61. La Di Da – The Internet
62. Doesn't Matter – Christine and the Queens
63. Moon River – Frank Ocean
64. No Tears Left to Cry – Ariana Grande
65. 1999 – Charli XCX and Troye Sivan
66. Only Love – Mary J. Blige
67. Pissed – Saweetie
68. Chun Li – Nicki Minaj
69. Powerglide – Rae Sremmurd featuring Juicy J
70. Call out My Name – The Weeknd
71. A$AP Forever – A$AP Rocky featuring Moby
72. Sword of Damocles – Rufus Wainwright
73. Back 2 You – Selena Gomez
74. Christmas Song – Phoebe Bridgers
75. The Gypsy Faerie Queen – Marianne Faithfull
76. Doves in the Wind – SZA featuring Kendrick Lamar
77. Suspirium – Thom Yorke
78. Charity – Courtney Barnett
79. If You're Over Me – Years & Years
80. My My My! – Troye Sivan
81. Time Today – Kero Kero Bonito
82. Tranquility Base Hotel & Casino – Arctic Monkeys
83. A Little Pain – Margo Price
84. Alfie's Song (Not So Typical Love Song) – Bleachers
85. In My Feelings – Drake
86. Django Jane – Janelle Monae
87. Meteorological – Guerilla Toss
88. Psycho – Post Malone featuring Ty Dolla $ign
89. Entitlement Crew – The Hold Steady
90. oh baby – lcd soundsystem
91. God's Plan – Drake
92. Electricity – Silk City with Dua Lipa
93. Trouble on Central – Buddy
94. BBO (Bad Bitches Only) – Migos featuring 21 Savage
95. MJ – Now, Now
96. Walk It Talk It – Migos featuring Drake
97. The Ways – Khalid featuring Swae Lee
98. Girlfriend – Christine and the Queens
99. Geyser – Mitski
Click here to like Tunes du Jour on Facebook!
Follow me on Instagram: @glennschwartz
My Top 99 Songs Of 2015
Herewith, my 99 favorite tracks of this year:
1. Can't Feel My Face – The Weeknd
2. Sugah Daddy – D'Angelo & the Vanguard
3. Peanut Butter Jelly – Galantis
4. Downtown – Macklemore & Ryan Lewis featuring Eric Nally, Melle Mel, Kool Moe Dee & Grandmaster Kaz
5. Fuck It All – Elle Varner
6. Therapy – Mary J. Blige
7. Coffee (Fucking) – Miguel
8. What Do You Mean? – Justin Bieber
9. Close Your Eyes (And Count to Fuck) – Run the Jewels featuring Zack De La Rocha
10. Hello – Adele
11. Only One – Kanye West featuring Paul McCartney
12. Depreston – Courtney Barnett
13. On the Regular – Shamir
14. Lampshades on Fire – Modest Mouse
15. Shine – Years & Years
16. Hotline Bling – Drake
17. Truffle Butter – Nicki Minaj featuring Drake and Lil Wayne
18. The Blacker the Berry – Kendrick Lamar
19. Dead Fox – Courtney Barnett
20. The Ground Walks, with Time in a Box – Modest Mouse
21. Uptown Funk – Mark Ronson featuring Bruno Mars
22. FourFiveSeconds – Rihanna featuring Kanye West and Paul McCartney
23. Teenage Talk – St. Vincent
24. Alright – Kendrick Lamar
25. Who U? – Dynas featuring Slick Rick
26. Friday I'm in Love – Yo La Tengo
27. Queen – Perfume Genius
28. King Kunta – Kendrick Lamar
29. Yoga – Janelle Monae featuring Jidenna
30. Dreams – Beck
31. Return to the Moon (Political Song for Didi Bloome to Sing, with Crescendo) – EL VY
32. The Love Within – Bloc Party
33. The Night Josh Tillman Came to Our Apt. – Father John Misty
34. Huarache Lights – Hot Chip
35. Dancing in the Dark – Hot Chip
36. B.O.B. – Macy Gray
37. Break the Rules – Charli XCX
38. Ex's & Oh's – Elle King
39. Stay Gold – First Aid Kit
40. Cool for the Summer – Demi Lovato
41. Girl Crush – Little Big Town
42. Nobody Really Cares if You Don't Go to the Party – Courtney Barnett
43. Little Red Wagon – Miranda Lambert
44. Cedar Lane – First Aid Kit
45. Jonathan – Christine and the Queens featuring Perfume Genius
46. Boxing Day Blues (Revisited) – Courtney Barnett
47. In for the Kill – Shamir
48. Biscuits – Kacey Musgraves
49. Can't Get Enough of Myself – Santigold featuring BC
50. You're So Beautiful – Empire Cast featuring Jussie Smollett
51. Ugly Cherries – PWR BTTM
52. Earned It (Fifty Shades of Grey) – The Weeknd
53. Leave a Trace – CHVRCHES
54. Freedom – Pharrell Williams
55. Betray My Heart – D'Angelo & the Vanguard
56. Snakeskin – Deerhunter
57. Really Love – D'Angelo & the Vanguard
58. I Can't Lose – Mark Ronson featuring Keyone Starr
59. Outta My Mind – the Arcs
60. Happy Idiot – TV on the Radio
61. Bad Blood – Ryan Adams
62. Nobody's Empire – Belle & Sebastian
63. Come – Jain
64. Stonemilker – Björk
65. Holding On – Julio Bashmore featuring Sam Dew
66. Go Out – Blur
67. Weight in Gold – Gallant
68. Chateau Lobby #4 (in C for Two Virgins) – Father John Misty
69. Coming Home – Leon Bridges
70. All Day – Kanye West featuring Theophilus London, Allan Kingdom and Paul McCartney
71. Glory – Common and John Legend
72. Need You Now – Hot Chip
73. Sausage – Lil Mama
74. Déjà Vu – Giorgio Moroder featuring Sia
75. Alone on Christmas Day – Phoenix featuring Bill Murray, Jason Schwartzmann, Buster Poindexter and Paul Shaffer
76. Smokin' & Drinkin' – Miranda Lambert featuring Little Big Town
77. She's Not Me – Jenny Lewis
78. Omen – Disclosure featuring Sam Smith
79. For Sale – Kendrick Lamar
80. NWA – Miguel featuring Kurupt
81. Times Square – Destroyer
82. I Put a Spell on You – Annie Lennox
83. 4 Degrees – ANOHNI
84. Go – The Chemical Brothers featuring Q-Tip
85. Sometimes – Heems
86. Bitch Better Have My Money – Rihanna
87. Runnin' (Lose It All) – Naughty Boy featuring Beyoncé and Arrow Benjamin
88. Smooth Sailin' – Leon Bridges
89. Hell – The Pains of Being Pure at Heart
90. Simple Things – Miguel
91. Shivers – Courtney Barnett
92. Tom's Diner – Giorgio Moroder featuring Britney Spears
93. Trap Queen – Fetty Wap
94. Tell Your Friends – The Weeknd
95. L$D – A$AP Rocky
96. These Walls – Kendrick Lamar featuring Bilal, Anna Wise and Thundercat
97. Love is Free – Robyn & La Bagatelle Magique
98. Country Down – Beck
99. Better in the Morning – Little Boots
Click here to like Tunes du Jour of Facebook!
Follow me on Twitter: @TunesDuJour
Giorgio By Moroder
"What the hell is this, Giorgio?"
That is what Donna Summer asked Giorgio Moroder, the producer who brought her to international fame with the track "Love to Love You Baby," upon hearing a song Moroder intended for her 1977 album I Remember Yesterday. The album's concept was to combine modern sounds with sounds reminiscent of past musical eras. The title track opens the album with a 1920s feel, which is followed by a fifties throwback and a sixties throwback. The song that perplexed Donna was intended to signify the future.
The synthesizer-based futuristic track was the b-side of the album's first single, a ballad entitled "Can't We Just Sit Down (And Talk It Over)." Releasing a ballad as the first single for the Disco Queen's new album was a strange move. The song failed to make the pop charts, though it did the top twenty of the r&b chart, Summer's first single to do so since "Love to Love You Baby."
In some foreign markets, the synth track was the single's A-side. It made noise, ultimately topping the charts in the U.K., the Netherlands, France, Australia, Italy, Belgium, and Austria. It reached #3 in Germany, where David Bowie was recording with produce Brian Eno. Bowie remembered Eno running into the studio with a copy of the song. Eno played it for Bowie, who recalled him saying "'This is it, look no further. This single is going to change the sound of club music for the next fifteen years.' Which was more or less right."
In the U.S. this B-side became the A-side, and reached #6 on the pop chart. Rolling Stone magazine included the track, entitled "I Feel Love," on its list of the 500 Greatest Songs of All-Time.
Today, Giorgio Moroder, who has four Grammy Awards and three Academy Awards to his credit, turns 75 years old. He has a new album, Déjà Vu, featuring guest vocalists such as Britney Spears (on a cover of Suzanne Vega's "Tom's Diner"), Kylie Minogue, Sia, Kelis, and Charli XCX, due to be released this June. Here are twenty career highlights.
Click here to like Tunes du Jour on Facebook!
Follow me on Facebook: @TunesDuJour
Glenn's Ten – January 28, 2015
It's been awhile since I posted Glenn's Ten, the ranking of my ten favorite songs of the week, so let me rectify that.
At number one is Elle Varner with "Fuck It All," which will be my wedding song. Elle is the only act in Glenn's Ten this week who is there for the first time. Long-missing D'Angelo surprise released an album in December, from which two tracks are in this week's tally. Kanye West surprise-released a single on New Year's Eve. That song, "Only One" was last week's #1. On it he is helped by Sir Paul McCartney, who makes his first Glenn's Ten appearance since "No More Lonely Nights" in 1984. Mary J. Blige has also been absent from Glenn's Ten for a number of years, but she is there now with "Therapy," a former #1.
Glenn's Ten for this week is:
1 – "Fuck It All" – Elle Varner
2 – "Sugah Daddy" – D'Angelo
3 – "Only One" – Kanye West featuring Paul McCartney
4 – "Therapy" – Mary J. Blige
5 – "Break the Rules" – Charli XCX
6 – "Little Red Wagon" – Miranda Lambert
7 – "Really Love" – D'Angelo
8 – "Earned It" – The Weeknd
9 – "Stay Gold" – First Aid Kit
10 – "She's Not Me" – Jenny Lewis
Today's playlist consists of those ten songs, followed by ten songs that were #1 on Glenn's Ten on this date in past years.
Click here to like Tunes du Jour on Facebook!
Follow me on Twitter: @TunesDuJour
Grammy Nominees Were Announced And I Need To Dance!
This year's Grammy nominations were announced this morning. Here they are:
Record of the Year
Iggy Azalea ft. Charli XCX – "Fancy"
Sia – "Chandelier"
Sam Smith – "Stay With Me (Darkchild Version)"
Taylor Swift – "Shake It Off"
Meghan Trainor – "All About That Bass"
Song of the Year
Same as Record of the Year, except instead of "Fancy" you've got Hozier's "Take Me to Church"
Album of the Year
They plan on announcing the nominees in this category tonight during the A Very Grammy Christmas television special. Ariana Grande, Maroon 5 and Album of the Year nominations? Cancel your Friday night plans!
Best New Artist
Iggy Azalea
Sam Smith
Some lady I've never heard of
Best Pop Vocal Album
Coldplay – Zzzzz
Miley Cyrus – Zzzzz
Ariana Grande – Zzzzz
Katy Perry – Zzzzz
Ed Sheeran – Zzzzz
Sam Smith – Zzzzz
Best Rock Album
Beck – Morning Phase
Ryan Adams – Ryan Adams
The Black Keys – Turn Blue
Tom Petty & The Heartbreakers – Hypnotic Eye
U2 – Are You Fucking Kidding Me?!?!
Best Urban Contemporary Album
Jhené Aiko – How Do You Pronounce That?
Beyoncé – I'm Now The Most Nominated Woman In Grammy History, So Bow Down Bitches
Chris Brown – Undeserved
Mali Music – Who?
Pharrell Williams – Gurl!
Best Country Album
Miranda Lambert – Platinum
+ four others
Best Spoken Word Album (a/k/a Best Audiobook)
Forget the titles; look at this list of nominated performers – James Franco, John Waters, Joan Rivers, Gloria Gaynor, Elizabeth Warren and Jimmy Carter! They better present this one on the telecast! Gurl!
Best Rock Song
Paramore – "Ain't It Fun"
Beck – "Blue Moon"
The Black Keys – "Jack White Better Not Be Nominated"
Ryan Adams – "Gimme Something Good"
Jack White – "The Black Keys Better Not Be Nominated"
Best R&B Song
Beyoncé featuring Jay-Z – "Drunk In Love"
Usher – "I'm Going to Lose to Beyoncé"
Chris Brown featuring Usher and Rick Ross – "I Don't Deserve a Nomination and I'm Going to Lose to Beyoncé"
Luke James featuring Rick Ross – "You Never Heard of Me and I'm Going to Lose to Beyoncé"
Jhené Aiko – "Though I Also Have an Accent over the Second E in My First Name I'm Going to Lose to Beyoncé"
Best Country Song
Miranda Lambert – "Automatic"
+ four others
Best Dance Recording
Seriously, there is a category for the best audiobook. The Grammy Awards' tag-line is "Music's Biggest Night." Unless Elizabeth Warren sang her memoirs this category should not exist.
This post doesn't cover all nominations. In total, the Grammy Awards have nominees in 12,623 categories, three of which are presented on the air. Tune in sometime in January or February to see who wins as well as a rare live television appearance from the reclusive Taylor Swift!
As for now, it's Friday, which is dance day on Tunes du Jour. As tomorrow is Ira Gershwin's 118th birthday, we'll kick off this week's dance party with Donna Summer, who by now may have dined with the famed lyricist.
Click here to like Tunes du Jour on Facebook!


text:
- China
Free indian girl massage Porn Videos | xHamster Erotic massage Jingling
- Quick Links
- Phone numbers of parlors sexual massage Jingling China 1805618
- Healing Massage - Full Body Japanese Massage Oil Relaxing Muscle To Relieving Stress (video)
- Phone numbers of parlors happy ending massage Jingling
Being the embodiment of your fantasies, these ideal bodies will only please you for hours. Cape Town Escorts. After that begins an erotic body massage.
Quick Links
Through the Internet the visitor can organize all his leisure for several hours ahead. The treatment is done in the nude, with body slides and it! Comment cannot be longer than characters. Wilmersdorf Mandy-private Super cute and super sexy The entire amount of information provided on the site or in the telephone mode is fully consistent with reality;.
Phone numbers of parlors sexual massage Jingling China 1805618
Description Exotic massage outdoor is given to a vip vlient with very smooth cock massage and free fucking with asian tight pussy.
Healing Massage - Full Body Japanese Massage Oil Relaxing Muscle To Relieving Stress
Phone numbers of parlors happy ending massage Jingling
Or leave your phone number and we will call you back. Home of Porn Download xhamster massage anal asian wife.
Erotic massage in Kiev's parlor "Simona".
|Sensual massages
|Top Erotic Pornstars
|Nude massage
|Popular categories
|Orgasm massage
|Our masseuses:
|Nude massage
|Village timal
|Sexual massage
|yes massage Searches
Confidentiality is Jingling to all customers.
here can cum Erotic their big tits and enjoy the sight of their jingling bells.
- It is especially important for single guys who want to find comfort in Jingling arms.
- Relax with Jingling enjoyment refers to premium services for the most demanding customers who do not always receive the necessary satisfaction with one masseuse.
- Thank you.
- Video Removed Undo.


text:
This year the Far South Under 13 Team competed at Yerrinbool. This tournament was tough from start to finish. Although we lost the first 4 games I was proud of the way the boys continued to battle and the way they handled themselves overcoming this setback. When you consider we had a bad run of injuries from the start resulting in us having only one sub plus several players carrying niggling injuries than you realise the magnitude of their effort and commitment to one another and the team.
We had created enough chances to win these games but did not take them when presented. We probably deserved more from this tournament with the type of football played but we have to learn that you have to take games at this level, no one is going to give you a win unless you take it when it is there for the taking. Players played in positions that they had not had a chance to play for a while and they all enjoyed this freedom. The enjoyment showed in their football with great team shape, better ball movement and creative plays that pleased all who witnessed them.
As coach of this team all I can say is that I enjoyed every minute of football the boys played and that I am proud of every player. Well done boys you gave me, your parents and the rest of the crowd at Yerrinbool 100% all week and your football was good to watch. The boys are a credit to their families, Associations and current teams that they play for, good luck for the rest of the season hope to see you around the grounds during the year.
Coach Dragan Blazevic
PS: Boys you made this an enjoyable tournament to watch and coach.