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Sign in with Facebook Other Sign in options. Bobby James, are dispatched to North Korea on a covert mission, all in an effort to take out a missile site Tom Sizemore stars in the most explosive Behind Enemy Lines yet! Seal Team 8 must fight their way through the Congo to defuse one of the greatest threats the world has ever known. A Navy navigator is shot down over enemy territory and is ruthlessly pursued by a secret police enforcer and the opposing troops. Meanwhile his commanding officer goes against orders in an attempt to rescue him.
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Personally, I didn't even know there was a sequel to the original, but considering this is a WWE produced film staring Mr Kennedy, who was I not to watch this? The storyline is pretty basic, navy SEALS organize an attack on Colombian Special Forces to clear their names and rescue a hostage which is one of their own. After watching this I can see why this was a straight to DVD release, it was really bad! Take a look at our favorite movie and TV stills from the past year.
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It is important to determine the enabling mechanisms that underlie locomotor performance to explain the evolutionary patterns and ecological success of animals. Our aim was to determine the extent to which calcium (Ca2+) handling dynamics modulate the contractile properties of isolated skeletal muscle, and whether the effects of changing Ca2+ handling dynamics in skeletal muscle are paralleled by changes in whole-animal sprint and sustained swimming performance. Carp (Cyprinus carpio) increased swimming speed by concomitant increases in tail-beat amplitude and frequency. Reducing Ca2+ release from the sarcoplasmic reticulum (SR) by blocking ryanodine receptors with dantrolene decreased isolated peak muscle force and was paralleled by a decrease in tail-beat frequency and whole-animal sprint performance. An increase in fatigue resistance following dantrolene treatment may reflect the reduced depletion of Ca2+ stores in the SR associated with lower ryanodine receptor (RyR) activity. Blocking RyRs may be detrimental by reducing force production and beneficial by reducing SR Ca2+ depletion so that there was no net effect on critical sustained swimming speed (Ucrit). In isolated muscle, there was no negative effect on force production of blocking Ca2+ release via dihydropyridine receptors (DHPRs) with nifedipine. Nifedipine decreased fatigue resistance of isolated muscle, which was paralleled by decreases in tail-beat frequency and Ucrit. However, sprint performance also decreased with DHPR inhibition, which may indicate a role in muscle contraction of the Ca2+ released by DHPR into the myocyte. Inhibiting sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) activity with thapsigargin decreased fatigue resistance, suggesting that SERCA activity is important in avoiding Ca2+ store depletion and fatigue. We have shown that different molecular mechanisms modulate the same muscle and whole-animal traits, which provides an explanatory model for the observed variations in locomotor performance within and between species.
Locomotor performance represents the non-cognitive dimension of animal behaviour and is important during intraspecific and interspecific interactions between individuals. Absolute performance capacity, such as increased fatigue resistance, influences ecological success and fitness by increasing success during escape from predators (Walker et al., 2005; Langerhans, 2009), in competitive aggressive interactions (Garland et al., 1990; Husak et al., 2006; Wilson et al., 2009) and in acquisition of resources (Bennett, 1991). In addition, the relative capacity for sprint and endurance performance is linked to behavioural modes such as pursuit versus sit-and-wait predation (Perry, 1999), sneaky mating versus prolonged courtship behaviour (Wilson et al., 2010) and even sporting success in long-distance versus sprint or strength disciplines (Van Damme et al., 2002). Selection acts on whole-animal traits such as sprint or endurance locomotor performance. However, the evolutionary process itself occurs at the level of genetic and molecular mechanisms (Lande and Arnold, 1983). Hence, sub-organismal traits are often used to explain the success of animals in particular environments or circumstances, and to predict the vulnerability of species to change (Gibb and Dickson, 2002; James et al., 2007; Claireaux and Lefrançois, 2007). However, the relationship between sub-organismal traits and whole-animal performance remains unresolved (Gibb and Dickson, 2002). It is important to determine the enabling mechanisms that underlie locomotor performance to explain evolutionary patterns by revealing the traits that selection may act upon (Arnold, 1983; Irschick and Garland, 2001; Irschick et al., 2008), and to assess constraints to locomotor performance (Dickinson et al., 2000; Le Galliard et al., 2004). The aim of this research was therefore to determine the extent to which calcium (Ca2+) handling dynamics modulate the contractile properties of isolated skeletal muscle, and whether the effects of changing calcium handling dynamics in skeletal muscle are paralleled by changes in whole-animal sprint and sustained swimming performance.
Locomotor performance depends principally on muscle performance, at least in species that have broadly similar morphological configurations (Vanhooydonck et al., 2001). Muscle performance depends on a number of extrinsic factors such as oxygen and nutrient supply by the cardiovascular system (Joyner and Coyle, 2008; Steinhausen et al., 2008), and intrinsic factors such as cellular metabolic capacities and myosin ATPase activities (Johnston et al., 1985; Johnson and Bennett, 1995; Johnston and Temple, 2002; Glanville and Seebacher, 2006), and the efficacy of Ca2+ handling dynamics (Berchtold et al., 2000; James et al., 2011). Ca2+ handling dynamics within the muscle cell are one of the most important intrinsic determinants of muscle performance (Berchtold et al., 2000). Differences in expression and activity of the Ca2+ release channels dihydropyridine receptor (DHPR) and ryanodine receptor (RyR), and Ca2+ re-sequestration by sarco(endo)plasmic reticulum ATPase (SERCA) can affect power output and fatigue resistance of isolated muscle (Fleming et al., 1990; Syme and Tonks, 2004; James et al., 2011). However, the impact of these molecules on whole-animal performance remains unresolved, although the increase in densities of DHPR and RyR with endurance training in some fish (Antilla et al., 2006) indicates that these molecules are important for whole-animal locomotion.
We tested the following hypotheses. (1) Inhibition of DHPR-mediated Ca2+ flow across the sarcolemma will have a limited effect on isolated muscle mechanics and sprint performance, but it will influence sustained swimming performance by its effect on the cardiovascular system. DHPR are voltage-gated Ca2+ channels, which, upon neural stimulation, release Ca2+ into the muscle cell and stimulate RyR (Meissner, 1994). In cardiac muscle, RyR activation is dependent on Ca2+ release by DHPR, whereas in skeletal muscle RyR is activated by direct interaction with DHPR, independent of Ca2+ release (Dirksen, 2009). (2) Slowing release of Ca2+ from the sarcoplasmic reticulum (SR) by blocking RyR will slow muscle contraction and decrease force production. The Ca2+ released into the muscle cell from the SR binds to troponin and thereby facilitates interaction between actin and myosin to cause muscle contraction (Berchtold et al., 2000). The rate of Ca2+ release may determine speed of muscle shortening and the peak force produced, both of which affect sprint performance (Hirata et al., 2007). We predicted that blocking RyR will decrease whole-animal sprint velocity but increase sustained swimming performance by slowing depletion of Ca2+ stores in the SR, which is a known mechanism of muscle fatigue (Stephenson et al., 1998; Allen et al., 2008). (3) Slowing Ca2+ re-uptake into the SR by blocking SERCA activity will decrease muscle relaxation rates, as well as reducing force production and fatigue resistance. We tested these hypotheses by conducting a series of experiments in which we used pharmacological manipulation of DHPR, RyR and SERCA to determine their effect on isolated muscle mechanics, swimming kinematics, and maximal sprint and sustained swimming performance in carp, Cyprinus carpio.
Study animals
Carp (Cyprinus carpio Linnaeus 1758; mean total length=88.90±1.8 mm; mean mass=18.03±0.91 g) were obtained from A5 Aquatics (Nuneaton, UK, N=40) and Australian Koi Farm (Bringelly, Australia, N=30). All fish were acclimated to 25°C for 1 month before experimentation, and were kept in groups of five to six fish in plastic tanks (645×423×276 mm) that were continuously filtered and aerated. Animals were fed commercial fish food daily, but were starved for 24 h before experimentation. All procedures had the approval of the University of Sydney Animal Ethics Committee (approval number L04/10-2009/2/5158) and were conducted in accordance with the British Home Office Animals (Scientific Procedures) Act 1986, schedule 1.
Swimming performance
We determined sprint velocity by filming the startling response of fish from above (using a Casio Exilim EX F1 camera recording at 60 frames s–1; Shriro Australia Pty Ltd, Sydney, Australia). Fish were introduced into a tank (405×600 mm) filled with water to a depth of 50 mm. When at rest, fish were startled by lightly tapping their tail with a stick. The ensuing escape response was filmed, and the fastest speed recorded in three escape responses was used as the maximum sprint velocity. We analysed videos in Tracker Video Analysis and Modeling Tool (Open Source Physics, www.opensource.physics.org).
Sustained swimming performance was measured as the critical sustained swimming speed (Ucrit) (Brett, 1964; Hammer, 1995) according to published protocols (Sinclair et al., 2011). We conducted a pilot study (N=6 fish) to determine the time interval between speed increments (ti=600 s), the speed increment (Ui=0.05 m s–1) and the initial flow rate (0.05 m s–1) so that fish were able to swim for several complete increments before exhaustion (Hammer, 1995). The swimming flume consisted of a 250×50 mm (length×diameter) clear Perspex® tube tightly fitted into the single exit of a Y-shaped rubber connector (total length=150 mm). Into each of the other openings of the Y-connector we inserted an inline submersible pump (Rule iL500 King Pumps, Miami, FL, USA; 12 V), achieving a tight fit. A plastic grid separated the Perspex® flume from the two pumps, and a bundle of hollow straws at the water inlet end of the flume, at the opposite end of the Y-connector, helped maintain laminar flow. The flume and pumps were submerged in a plastic tank (645×423×276 mm). We used a variable DC power source (MP3090, Powertech, Jaycar, Sydney, Australia) to adjust the flow speed, which was calibrated using a flow meter (FP101, Global Water, Gold River, CA, USA).
We determined the sensitivities of sprint and sustained swimming performance to the activities of DHPR and RyR by exposing fish to solutions of nifedipine (Sigma-Aldrich, Castle Hill, NSW, Australia) (Houston 1986) and dantrolene (Sigma-Aldrich) (Van Winkle 1976; Krause et al., 2004), respectively. There are no differences in the principal Ca2+ handling mechanisms between fibre types, although they differ in their relative density of DHPR and RyR; therefore, nifedipine and dantrolene will not bind preferentially to one or the other (Antilla et al., 2007; Lee et al., 1991; Berchthold et al., 2000). We estimated initial doses from our previous work on zebrafish (Seebacher and Walter, 2012); these doses were based on published data evaluating the effects of dantrolene and nifedipine in mammals using oral administration [nifedipine, approximately 5–20 μmol l–1; dantrolene, 1–100 μmol l–1 (Dykes, 1975; Foster, 1983; Houston, 1986)]. When presented orally, both drugs act within minutes and their half-life is approximately 10–12 and 2–3 h, respectively (Dykes, 1975; Foster, 1983). We first immersed fish in 30 μmol l–1 of nifedipine (N=8) or 150 μmol l–1 dantrolene (N=8) for 1 h before swimming performance tests. Subsequently, we increased doses to 50 μmol l–1 nifedipine and 300 μmol l–1 dantrolene. We used the same fish for different drug doses, starting at the lower dose and leaving 24 h between trials, which is sufficient for the effects of the initial doses to wear off (Dykes 1975; Foster et al., 1983). Additionally, we determined swimming performance in a control group (N=8) that was not treated with drugs, and to which we compared the drug treatments.
We determined tail-beat amplitude and frequency as a measure of in situ muscle performance during locomotion. We filmed fish (with a Casio Exilim Ex F1 camera filming at 60 frames s–1) swimming steadily at 0.15, 0.25 and 0.35 m s–1, and analysed video in Tracker software. We measured amplitude as the maximal displacement of the tail at the peduncle during one tail-beat cycle. Similarly, we determined period as the time taken for the tail to complete one cycle, and calculated frequency as 1/period. All swimming performance measures were taken at 25°C.
Muscle biomechanics
Fish used to determine biomechanics of isolated muscle were euthanized via a blow to the head, transection of the spinal cord and pithing. A bundle of caudal muscle fibres was dissected from each side of the fish (for subsequent muscle mechanics) in cooled (<5°C), oxygenated (95% O2; 5% CO2) carp Ringer's solution [composition in mmol l–1: NaCl 115.7, sodium pyruvate 8.4, KCl 2.7, MgCl2 1.2, NaHCO3 5.6, NaH2PO4 0.64, HEPES sodium salt 3.2, HEPES 0.97, CaCl2 2.1, pH 7.4 at 20°C (Johnson and Bennett, 1995; Wakeling et al., 2000)]. An aluminium foil T-clip was wrapped round either end of each muscle fibre bundle.
Isometric studies were used to determine the twitch and tetanus kinetics of the isolated caudal muscle fibre bundles. The following methodology was used simultaneously on two Aurora Scientific (Aurora, ON, Canada) setups to compare mechanical performance between the muscle preparations from each side of the fish (one muscle preparation acting as a control, one as drug treatment). The aluminium foil clips were attached at one end of the muscle fibre bundle to a force transducer (400A, Aurora Scientific), and at the other end to a motor arm (322C-I, Aurora Scientific). The muscle preparation was then allowed to equilibrate within the organ bath at 25±0.5°C for 10 min in circulating, oxygenated (95% O2; 5% CO2) carp Ringer's solution. The preparation was then held at constant length and subjected to a series of 1.0 ms pulse width stimuli via parallel platinum electrodes to generate a series of twitches. Stimulus amplitude (50–70 mA) and muscle length were adjusted to determine the stimulation parameters and muscle length corresponding to maximal isometric twitch force. An isometric tetanic force response was elicited by subjecting the muscle to a 300 ms train of stimulation. Stimulation frequency (110–130 Hz) was altered to determine maximal tetanic force. Time to half peak tetanic force (referred to as tetanus activation time) and time from last stimulus to half tetanic force relaxation (referred to as tetanus relaxation time) were measured via AS1600A analysis software (Aurora Scientific). Each tetanus exhibited a distinct peak on the left-hand side followed by a drop in force, then a plateau in force prior to relaxation (Fig. 1). Therefore, peak tetanic force was measured on the left- and right-hand side of each tetanus to monitor whether changes in force, due to fatigue or drug treatment, differentially affected the peak force produced in these different regions of the tetanus. A rest period of 5 min was allowed between each tetanic response. We calculated rates of force production (peak tetanic force/2× time to half peak tetanus) and muscle relaxation (peak tetanic force/2× time from last stimulus to half relaxation) as measures of the contractile performance of muscle.
After the maximal tetanus was achieved, the control Ringer's solution was replaced by either fresh control Ringer's solution (for the control muscle fibre preparation) or 50 μmol l–1 dantrolene (Van Winkle, 1976; Fruen et al., 1997), 10 μmol l–1 thapsigargin (Kurebayashi and Ogawa, 2001; Galli et al., 2006) or 10 μmol l–1 nifedipine Ringer's solution (Weigl et al., 2000) (all drugs purchased from Sigma-Aldrich). Maximal tetanus responses were determined after 10 min of incubation in the drug treatment.
After a further 5 min rest, fatigue resistance was determined by subjecting the muscle preparation to a series of tetani, each of 300 ms stimulation duration, at a rate of one tetanus per second for 200 s. We also determined fatigue in muscle from an additional, independent control group (N=10), to which we compared the drug treatments (see Statistical analysis below).
Statistical analysis
Critical sustained swimming speed (Ucrit) and sprint velocity of fish treated with dantrolene or nifedipine were compared with control fish using t-tests. Kinematics data were analysed by ANOVA with treatment as a fixed factor and water flow velocity as a repeated measure; we used Pillai's trace as the test statistic to determine significance for the repeated measure. We performed separate analyses for the dantrolene and nifedipine treatments. Of the nifedipine-treated fish, only four reached swimming velocities of 0.25 m s–1, and only two reached 0.35 m s–1. Hence, we could not analyse the effect of nifedipine of swimming kinematics at 0.35 m s–1.
Muscle mechanics data were analysed with paired t-tests, comparing performance of the untreated control muscle with that of the drug-treated muscle. We performed separate analyses for dantrolene, thapsigargin and nifedipine treatments. We analysed muscle fatigue by comparing each treatment with the control at tetanus numbers 10, 50, 100 and 200. Data were expressed as percent of the force produced in the first tetanus. We used PERMANOVA (in Primer 6 software, Primer-E, Plymouth, UK) with treatment (control and each drug treatment) as a fixed factor and tetanus number as a random factor. The truncated product method (Zaykin et al., 2002) was used to combine all the P-values in this study to determine whether there is a bias from multiple hypothesis testing. The truncated product method P-value was <0.0001, showing that the results are not biased.
Swimming performance
Neither Ucrit nor sprint performance changed with exposure to 150 μmol l–1 dantrolene (t=1.59, P=0.13 and t=0.25, P=0.81, respectively). A dantrolene concentration of 300 μmol l–1 did not affect Ucrit (t=1.33, P=0.20), but it significantly decreased sprint velocity (t=2.28, P<0.05; Fig. 2). Exposure to 30 μmol l–1 nifedipine did not affect swimming performance (Ucrit: t=1.32, P=0.21; sprint velocity: t=1.68, P=0.12), but 50 μmol l–1 nifedipine significantly reduced Ucrit (t=2.79, P<0.02) and sprint velocity (t=4.99, P<0.0001; Fig. 2).
Swimming kinematics
Tail-beat amplitude increased significantly with increasing water flow (F2,10=5.52, P<0.03; Fig. 3A), and dantrolene did not affect tail-beat amplitude compared with control fish (F1,11=1.98, P=0.19). Tail-beat frequency increased with increasing flow (F2,10=25.57, P<0.0001), but the increase was significantly less in dantrolene-treated fish compared with controls (interaction between treatment and flow velocity, F2,10=4.75, P<0.05; Fig. 3B).
Similar to the dantrolene treatment, amplitude did not change as a result of the nifedipine treatment (F1,9=0.012, P=0.92; Fig. 3A), but there was a significant interaction between flow speed and frequency as a result of nifedipine treatment (F1,9=6.49, P<0.05; Fig. 3B).
Effect of drug treatments on muscle biomechanics
Exposure to dantrolene significantly reduced tetanus relaxation times, and also decreased peak tetanus force (Fig. 4, left column; Table 1). However, dantrolene did not affect activation time, or the rates of muscle activation or relaxation (Figs 4, 5, Table 1). Thapsigargin did not significantly affect tetanus activation or relaxation times, or peak tetanus force (Fig. 4, right column; Table 1). Thapsigargin did, however, significantly increase the rates of force production and muscle relaxation (Fig. 5, Table 1). Interestingly, nifedipine increased peak tetanic force, but had no effect on muscle activation and relaxation times (Fig. 4, centre column; Table 1), so that rates of force production and relaxation also increased significantly following nifedipine treatment (Fig. 5, Table 1).
During the fatigue runs, tetanus force of the left- and right-hand sides decreased with increasing tetanus number in all treatments (all pseudo F3,72>12.0, P<0.001; Fig. 6). Dantrolene had no effect on the left-hand side of the tetanus (pseudo F1,72=0.30, P=0.78; Fig. 6A) but significantly attenuated the decline in force production (i.e. it increased fatigue resistance) of the right-hand side compared with controls (pseudo F1,72=15.63, P<0.02; Fig. 6B). Thapsigargin did not affect force production of the left-hand side of the tetanus compared with controls (pseudo F1,72=3.93, P=0.12; Fig. 6A), but force production declined more rapidly (i.e. fatigue resistance was decreased) on the right-hand side following thapsigargin treatment (pseudo F1,72=4.91, P<0.05; Fig. 6B). Nifedipine caused a significantly faster decline in force production with increasing tetanus number (i.e. decreased fatigue resistance) in both the left- and right-hand sides compared with controls (left: interaction pseudo F3,72=9.38, P<0.001; right: interaction pseudo F3,72=8.21, P<0.001; Fig. 6).
High force production and rapid rates of muscle activation and relaxation are linked mechanistically to high sprint performance (Spierts and Van Leeuwen, 1999). As we predicted, the decrease in isolated peak muscle force following RyR blockade was paralleled by a decrease in whole-animal sprint performance. The decrease in force can be explained by reduced Ca2+ release from the SR, decreasing the number of actinomyosin cross-bridges (Berchtold et al., 2000). However, reduced RyR activity following dantrolene treatment did not decrease muscle activation times. Unexpectedly, dantrolene reduced muscle relaxation times (Table 2). The latter may reflect faster re-sequestration of Ca2+ into the SR as a result of the lower concentration of Ca2+ associated with reduced RyR activity and force production. Accordingly, rates of muscle relaxation remained unaffected by dantrolene. In contrast, rates of force production and relaxation decreased significantly in rat muscle following dantrolene treatment (James et al., 2011). The increase in fatigue resistance following dantrolene treatment may reflect the reduced depletion of Ca2+ stores in the SR associated with lower RyR activity (Stephenson et al., 1998); note that dantrolene treatment does not block RyR completely, so there will be residual Ca2+ release from the SR (van Winkle, 1976). However, this increase in fatigue resistance of isolated muscle did not translate to increased sustained swimming performance. Ucrit and burst swimming velocities of our control fish were similar to those reported in the literature (Heap and Goldspinck, 1986; Wakeling et al., 2000). Carp increased swimming speed by concomitant increases in tail-beat amplitude and frequency. The reduction in tail-beat frequency at higher swimming speed when RyRs were blocked indicates that the whole muscle became slower during swimming and/or the reduction in muscle force production during dantrolene treatment compromised the fish's ability to overcome the greater drag forces involved at higher tail-beat frequencies. This observation is not reflected in the biomechanics of a single tetanus. There was an indication that activation rates of the tetanus decreased when RyRs were blocked, but this was not significant (P=0.062). However, this reduction in activation rates may become more pronounced with repeated tetanus stimulation, and muscle activation time and swimming speed in fish are often correlated (Wardle, 1975). RyRs can affect muscle in several ways and, as outlined above, increased activity increases force production but at the same time depletes Ca2+ stores in the SR, thereby inducing fatigue. Hence, at the same time, blocking RyRs may be detrimental by reducing force production and beneficial by reducing SR Ca2+ depletion, so that there is no net effect on Ucrit. This is in contrast to sprint performance, where SR Ca2+ depletion would be relatively unimportant, so that blocking RyR would have a detrimental effect only, as observed.
DHPRs mediate excitation–contraction coupling and release Ca2+ into the myocyte upon nerve stimulation. In heart muscle, DHPRs stimulate RyRs by Ca2+ release into the myocyte (Shiels et al., 1999; Tiitu and Vornanen, 2003). In contrast, activation of RyR by DHPR in skeletal muscle is achieved by mechanical coupling between the two receptors, which is independent from the Ca2+ release (Dirksen, 2009). Nifedipine blocks the Ca2+ current across the sarcolemma but does not inhibit the direct interaction between DHPR and RyR (Fleckenstein, 1983). Hence, in isolated muscle there is no negative effect of nifedipine on force production. On the contrary, nifedipine increased force production, but also decreased fatigue resistance of isolated muscle. These responses were consistent across all animals but are not easily explained. It may be that the Ca2+ released by DHPR has an inhibitory effect on RyR at the same time as the molecular interaction stimulates RyR, thereby maintaining a 'tone' on RyR activity. The activity of RyR is regulated by Ca2+ concentrations, whereby the receptor is activated by low [Ca2+] (0.5 μmol l–1) and inhibited by higher [Ca2+] (0.15 mmol l–1) (Berchtold et al., 2000). This scenario would explain the data from our isolated muscle experiments, but is highly speculative and must be confirmed experimentally.
Reduced fatigue resistance of isolated muscle following nifedipine treatment is paralleled by decreases in tail-beat frequency and Ucrit. These findings indicate a direct effect of muscle mechanics on whole-animal performance. The decrease in Ucrit may have been further enhanced by the depressing effect of DHPR inhibition on the cardiovascular system (Fleckenstein, 1983; Vornanen, 1997; Galli et al., 2006). However, sprint performance also decreased with DHPR inhibition, which may indicate a role of the cardiovascular system in facilitating burst activity, which is unlikely, or a role in muscle contraction of the Ca2+ released by DHPR into the myocyte. The fact that concentrations of DHPR increase with endurance training (Antilla et al., 2006), and that sprint performance decreases with a decrease in DHPR concentration (Hirata et al., 2007) confirms that there is a positive relationship between the density of this receptor and whole-animal locomotion. Undoubtedly, this role is in large part mediated by the direct interaction between DHPR and RyR, but our data indicate that there is also a more complex functional relationship that does not involve this direct interaction (i.e. as indicated by the effect of nifedipine). In addition to its role as a rapid-acting voltage sensor during excitation–contraction coupling, the DHPR is also a slow-acting voltage-gated L-type Ca2+ channel (Dirksen, 2009). This slow mode of action may be important in replenishing Ca2+ stores (Nakai et al., 1996; Bannister et al., 2009; Dirksen, 2009), which is essential to increase fatigue resistance (Kurebayashi and Ogawa, 2001; Pan et al., 2002). The fact that tail-beat frequency decreased and few fish continued swimming at moderate (compared with control) water flow velocities is consistent with fatigue induced by depletion of Ca2+ stores.
The moderate, but significant, increases in activation and relaxation rates of isolated muscle following thapsigargin treatment are unexpected. For a single tetanus, it may point towards increased reliance on alternative and faster Ca2+ sequestration mechanisms, such as parvalbumin (Arif, 2008), when SERCA activity is low. Single tetanus force production of isolated muscle was not affected by thapsigargin treatment, which indicates that Ca2+ stores in the sarcoplasmic reticulum are not depleted during a single tetanus to the extent that reduced SERCA activity, and thus slower replenishing of Ca2+ stores, would inhibit force production (Allen et al., 2008). Again, re-sequestration of Ca2+ from the cytosol by parvalbumin would facilitate muscle relaxation at least in the short term, and may thereby help maintain force production and swimming performance (Seebacher and Walter, 2012). Isolated mouse soleus muscle in which SERCA2 gene expression was disrupted also showed no decrease in force production, although there was an increase in relaxation times (Sjåland et al., 2011), which may indicate that other SERCA isoforms partially compensated for the reduction in SERCA2 activity, and that store depletion did not reduce force production over the time frame of measurement. In the longer term, however, thapsigargin treatment decreased fatigue resistance of carp muscle, suggesting that ultimately SERCA activity is essential in re-uptake of Ca2+ into the SR to avoid store depletion and fatigue. Again, there are differences between our findings from carp here and our previous work on rat muscle, where thapsigargin did not affect rates of force production or muscle relaxation, although as in carp it did reduce fatigue resistance (James et al., 2011).
We have shown that different molecular mechanisms involved in Ca2+ cycling during excitation–contraction coupling affect the same whole-animal traits, and that the same molecular trait, such as RyR activity, affects whole-animal locomotion differently in different species. The strength of uncovering the mechanistic basis of whole-animal function lies in providing explanatory models for observed variation within and between species. Hence, questions addressing the evolutionary processes that lead to differences between species or populations should focus on underlying mechanisms rather than solely on the whole-organism trait.
This research was in part supported by an Australian Research Council grant to F.S.
- © 2012.


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Who has the other lost panels of Cimabue's masterpiece?
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Arthur's Pass
Since the Arthur's Pass road is supposed to be the highest and most spectacular highway across the Southern Alps we had to do it. And it was really spectacular.
Unfortunately the water level at the Cave Stream was too high to do the cave. Given that we are not experienced cavers we decided to walk around and have a look at the exit. It was good that we didn't decide to enter the cave since the water was pushing out of it angrily at the exit.
Castle Hill with its large limestone rocks was impressive so we climbed up to have a beautiful view at the surrounding snow capped mountains. The Otira Viaduct – a long bridge - was worth a picture even if we had to watch out for the curious kea – the mountain parrot – which loves to nibble away the rubber from your car.
At the Lake Pearson we set up camp and enjoyed the mountain reflections in the lake.


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It is ceaselessly flattering to be contacted by so many people requesting Greg's food for parties and various events. Thank you for thinking of us time and again, but we are no longer operating the truck, nor are we catering, or personal cheffing.
**We no longer are using the "beachstreetsandwiches.com" domain which means many of the links on this site do not work. Forgive us.
We thought it was the sub-zero temperatures, wind, and fatigue that made the tarp feel gigantic when we covered the truck and wrapped it for protection. Twice. But, as you can see, our tarp is about a square acre. That means unwieldy is an understatement. But it surely did the job. Devil Winter 2010-11 did no damage to the Beach Street Sandwiches truck. Thank you, mega-tarp, for a job well done.
Welcome back to Free Food Truck Consulting by Beach Street Sandwiches. Dear everyone sending email and calling: Wow. Doing what we can to respond and dispense our brilliant advice. Something all of you aspiring food truck (and cart) owners should consider: It's mid-April. The Friday of Memorial Day weekend is 42 days away. That's six weeks. If your food truck is ready to go, congratulations. If you're wavering between building new and buying used, Buy Used! Unless you're not in a rush to open.
"Imagine this is an informational session," a woman said to the frustrated person next to her. "You can't come in here expecting to accomplish anything."
She was talking about the NYC Licensing Center and she was right. Worth mentioning because of the number of people who come to BeachStreetSandwiches.com seeking "how to open a food truck in NYC" info. In fact, here's a link to the site's most visited post: How To Open a Food Truck in New York City.
As you can imagine, we eat sandwiches everywhere we go, because we love them and because we like to see what other people are doing. We had a feeling we'd be pleased at Il Cane Rosso. When sandwiches are made/created by chefs - Daniel Patterson (Coi) and Lauren Kiino (met DP doing a stage at Coi; was long time Chef de Cuisine at Delfina) - chances are high that 'feeling we'd be pleased' will be realized. It was. They were. We were. Chef-created sandwiches rock.
Pictured: Marin Sun Farms Slow Cooked Pork with chile-lime salsa, shaved fennel, and cilantro salad. And the Bay Bridge.
Does "Live!" imply "happening at this moment"? Because we want that title to mean "in motion" (has the characteristics of something living). So here it is... Chef Greg McCarty in motion...
You realize how unobservant you are when you finally start noticing things. Until we got into the vending business we paid scant attention to the arrivals and departures of New York City's trucks and carts. This is about 9:30am, Central Park South near 5th Avenue (Grand Army Plaza). The horses aren't even out yet (though the horse-protection-activists are). Notice the bag of ice. Upon seeing it Sara considered hugging these gentlemen and calling them brothers. Vendor civil wars (turf, primarily) be damned; we're all brethren.
'Cause you wonder where we are
And you wonder what we do
Are you somewhere feeling hungry,
or is someone feeding you?
Tell us how to win your heart
For we have some items that are new
But let us start by saying, we miss you too
...and we're doing private events...
The BEACH STREET SANDWICHES truck is Closed. If you're looking for updates, changes, news, reincarnation possibilities, Twitter.com/BeachStSandwich is a great way to find us. We also update here: facebook.com/BeachStreetSandwiches.
If you're looking for food by Chef Greg McCarty, he's back in NYC. Stay tuned.
Thanks to all of our fans for wishing us back. Beach Street Sandwiches misses you too. We don't want you to go sandwichless without us so suggest you make your way to Deli Unlimited (1099 Boston Post Road, Guilford, CT, 203-453-2473). Some of our off-truck favorites are made there, and you can get gas too. Hee hee.
Vita Mix very pretty and the Vita Prep3 is sweet
and the fruit of Chef's labor is deliciousible to eat.
Thank you again to Vita Mix for the blender loan. Life is easier when you have the right equipment. Pictured: The making of Leek Puree. This is the one you want, says Vita Mix, and we could not agree more. As soon as we sort out 2010, we're going to start 2011 spending. Yea Section 179, one of the few stimulus incentives that actually applies to us, and helps us.
Yea! Your favorite Chef, Greg McCarty, just won best US Chef in a competition held in NYC at the International Restaurant Show. Know what that means? Besides what you already knew - that he is amazing, that everything he touches turns to deliciously edible gold? It means we're going to Prince Edward Island in September, sans truck, to the PEI Shellfish Festival.Does Chef have enough Newfie (that's slang for Newfoundland, pronounced NewfoundLAND, btw, where he attended elementary and high school) left in him to woo the Canadian judges? And enough Arkansas (birthplace + childhood summer home) to kick the butts of his competitors? Survey says: Yes!
So that's what we've been doing while the truck is closed. Plus sending out press releases with errors and typos (the brilliant writer gets very excited by the success of her incredible partner). Plus possibly finding a new wrap for the Breakfast All Day sandwich, and a 2-drink-holder-bag. Plus owning a Vita-Prep 3 for 24-hours courtesy of Vitamix. More about that to come. And videos! Thanks to KLT Productions. Stay tuned.
Still reading? How nice of you. It was Chef's PEI Blue Mussels Stew, a dish made with miso and morels, that won Most Creative and Best Use of Mussels to confirm this top spot.
Some of the CT Specialty Food Association's 2011 Product Awards Competition Winners:
1st Place - Pickle, Relish or Tapenade:
Dilly Beans (pictured)
made by Dondero Orchards, LLC, South Glastonbury, CT
1st Place - Packaging:
The Farmer's Cow Milk
created by The Farmer's Cow, Lebanon, CT
1st Place -Non-Alcoholic Beverage:
Black Currant Juice (pictured)
made by Maple Lane Farms, Preston, CT
1st Place - Ice Cream/Gelato:
Vanilla Ice Cream
made by Salem Valley Farms Ice Cream, Salem, CT
1st Place - Fruit or Vegetable:
grown by Urban Oaks Farm, New Britain, CT
1st Place - Dessert – Cookie:
Vanilla Chocolate Chunk Ice Cream Slider
made by J. Foster Ice Cream, Avon, CT
Click for more about the CT Specialty Food Product Association and Awards.
The thing about the Night Drop is this - it does not stop being scary. We'd like to say we trust everyone and have faith and all that but, really, we just keep waiting for the day when our deposit was not deposited, or the amount is changed, or something. And when/if such a situation occurs, you know an investigation follows, and even if it's all straightened out, it takes too long for a small business, or anyone else who wants their cash to be where it belongs: available.
That said, BEACH STREET SANDWICHES IS CLOSED, once again. Thanks to everyone who made our recent night drop possible. We're open for PRIVATE EVENTS ONLY (that means Catering, but no Drop Offs) throughout winter.
Scattered Flurries, Flurries, Chance of Snow Showers...How to Plan?
Changes to the-e-fair will be decided by 4pm. Check Twitter/BeachStSandwich or Fbook/BeachStreetSandwiches.
We need help - what should we call the new sandwich? Here (in words, not picture; that's just a tease) are some of the components: chicken, goat cheese & walnut spread, honey dijon mustard dressing. We need to decide by Thursday so we can make the Menu for the-e-fair. Leave suggestions in the Comments below. Thanks in advance. See you Saturday.


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By Lazy Girl
/ in Desert,Diets,Quick and Easy
/ tags Gluten Free, Keto, Keto diet, Keto Recipe, Ketogenic, Low Carb, Low Carb Recipe
So after making the most delicious keto chocolate chip cookie ever, I was thinking how could I make this dessert any better? With a sweet Lemon Cream Cheese sandwiched between soft, chocolatey cookies, this foolproof recipe for Keto Lemon Cream Cookie Sandwiches will satisfy any craving. Make these low-carb & gluten-free treats in less than 30 minutes!
These keto-friendly chocolate cookies are great fun to bake with kids too. Give them the Lemon Cream Cheese Filling to make, they'll have fun licking the bowl clean. I know I did!
Keto Lemon Cream Cookie Sandwiches Recipe
With a sweet Lemon Cream Cheese sandwiched between soft, chocolatey cookies, this foolproof recipe for Keto Lemon Cream Cookie Sandwiches will satisfy any craving.
Servings 14 sandwiches
Calories 108kcal
Chocolate Chip Cookies
- 2 tbsp Butter, melted
- 2 tbsp unsweetened Natural Peanut Butter, I use this brand
- 1 cup finely ground almond flour, I use this brand
- ¼ cup sugar-free chocolate chips, I use this brand
- ½ cup granular Swerve
- 1 egg
- ¼ tsp salt
- ¼ tsp baking soda
- ½ tsp baking powder
- 2 tbsp coconut oil, I use this brand
- 1 tsp pure vanilla extract, I use this brand
Lemon Cream Cheese Filling
- 4 oz cream cheese (room temperature)
- ¼ cup unsalted butter (room temperature)
- ¼ tsp lemon extract
- 1 tsp fresh lemon juice
- ½ tsp fresh lemon zest
- ⅓ cup Swerve Confectioner's
For Chocolate Chip Cookies
- Preheat oven to 350° F.
- In a medium bowl mix melted butter, peanut butter, swerve, vanilla, coconut oil, and egg until very smooth, set aside.
- In a separate bowl mix almond flour, baking powder, baking soda, and salt until well combined.
- Add flour mixture to butter mixture and mix until combined.
- Add chocolate chips to the combined mixture and stir until just incorporated.
- Roll the dough into around 28 balls, and place balls evenly across both cookie sheet lined with a Silpat, or parchment paper. With your fingers, lightly flatten the dough, as it will not spread during baking.
- Place on a cookie tray, and bake on the center rack 10-13 minutes. Let cool an additional
10 minutes before handling, as they are very delicate at first but firm up completely once cool.
For Lemon Cream Cheese Filling
- In an electric mixer or with a hand mixer on medium-high speed, mix cream cheese until smooth. Then add Swerve Confectioners and beat until smooth. Add remaining ingredients and beat on medium high for 10 minutes. Provide fluffiness and helps combine flavors. Place into icing bag with a Wilton 1A Round Tip.
- Take two individual cookies. With the first cookie pipe about a quarter size of the filling onto the center of the cookie. Place the other cookie on top of cookie with filling and squeeze together. Set aside, and follow this process for all cookies until you have around 14 lemon cream sandwiches.
Tip: Cookies can sit out on the counter for a few hours and become a little more firm if you have the time and makes them a bit crisper. You can also use chocolate chips for decoration.
NutritionPer serving (1 sandwich): Calories 109; Total Fat 11.2g; Saturated Fat 6.8g; Cholesterol 34mg; Cholesterol 34mg; Total Carbohydrate 5.5g; Dietary Fiber 0.2g; Total Sugars 4.5g; Protein 1.6g;
If you like this recipe share it with your friends and (..of course..) pin it for later.
Leave a Reply


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DISTANCE: 10.02 km DURATION: 1:08:12
Today Lincoln and I got as close to Edworthy Park as we could without running into the off-leash areas. The change of scenery meant we were stopping much more frequently so that Lincoln could catch up on the neighbourhood's' gossip and leave some of his own! That's ok though, the humidity was pretty high during the first half of our run so a slower pace was just what we needed. Lincoln really wanted to go down into the park, but I had to be the adult and say that we were road runners today - no trails! Despite staying away from off-leash areas, there were tons of dogs out! Lincoln was great, and at most would just prance beside me. Another highlight was a Stampede breakfast that had a live band! We watched the festivities for a minute before moving on. Once we got close to home, we weaved around to avoid dogs and had a little cool-down walk outside his house. These runs just seem to fly by! Thanks for the great morning Lincoln! 1 poop and 6-8 pees.
Runner: Laura
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November 2019


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Mr. Shakul Garg
Spacious room and very comfortable . Improve in the neatness of towels .
With a below average breakfast buffet, I won't be returning to this hotel.
Mr. Pradeep Parekh
I booked the luxury suite for my wedding anniversary and from my first contact everything went well. First, I made my reservation with Receptionist, who is so sweet, understanding, and bubbly. She was knowledgeable of the area, which helped a lot! ……
If you are searching for trusted tour operator in East Africa, then please be sure to check out African Sermon and its Kenya and Tanzania combined safaris here: africansermonsafaris.com


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fannie mae high cost areas Fannie Mae raises fees it charges lenders – Fannie Mae today outlined a. that's not counting closing costs, said Pava Leyrer, president of Heritage National Mortgage in Randville, Mich. Under the revamped fee scale, even a borrower with a.
Both Fannie Mae's Homestyle loan and the FHA 203K renovation mortgage allow you to borrow based on the improved value of the property. That means a higher loan amount to cover renovation costs.
Whether you choose an FHA or Fannie Mae loan, understand that neither the. FHA will take a close look at your total amount of debt compared to your income.
The FHA, Fannie Mae (Federal National Mortgage Association) and Freddie Mac (the Federal Home Loan Mortgage Corporation) do not make.
Fannie Mae Eligibility. Financing guaranteed by Fannie Mae are generally not as forgiving on their credit and down payment standards as FHA loans. That is the reason that many first-time home buyers with limited credit and down payment go with FHA loans. Fannie Mae generally requires a minimum FICO of 620 to get a fixed rate mortgage.
For certain Fannie Mae multi-class structured securities backed by FHA-insured, VA-guaranteed, and USDA-guaranteed government mortgage loans, the servicer is permitted to reduce the interest rate of any mortgage loan without removing the loan from the trust.
Home prices may come under downward pressure should the Consumer Financial Protection Bureau allow the "GSE Patch" for Fannie.
Max Conforming Loan Amount In California Last week, mortgage broker and Inman writer Lou Barnes opined that it was time for the Federal Housing Finance Agency (FHFA) to consider raising the conforming loan limit – the maximum amount of money.
Fannie Mae and Freddie Mac are big players in the mortgage industry.. Fannie and Freddie also keep some loans on their own books.. 2017 – 3 min read FHA Loan With 3.5% Down vs Conventional.
Confused by FHA, Freddie Mac and Fannie Mae? Me too. I recently had a visit from a friend who asked if he qualified for the HARP program, the Home Affordable Refinance Program (also known as HAMP.
Vs mae fannie fha Loan – Logancountywv – – The difference between Fannie Mae and FHA is FHA is a loan program that is guaranteed by our government. If you default on your loan and it goes to foreclosure, the bank uses the insurance the government provided on the loan to retain the remaining balance of what wasn't collected at.
Fannie Mae loans are not as forgiving in credit or down payment requirements as FHA loans. Fannie Mae requires a minimum credit score of 620 for fixed-rate mortgages and 640 for adjustable-rate.
Conforming Loan Limits 2017 Fannie Mae High Cost Areas Fannie Mae raises fees it charges lenders – Fannie Mae today outlined a. that's not counting closing costs, said Pava Leyrer, president of Heritage National Mortgage in Randville, Mich. Under the revamped fee scale, even a borrower with a.conforming loan limits increased for 2017 – apmortgage.com – Conforming Loan Limits Increased for 2017. The loans will vary by county, but for most of the United States, the 2017 maximum loan limit for one-unit properties will be $424,100, an increase from $417,000 (the level set back in 2006).


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National Geographic : 1934 Dec
THE NATIONAL GEOGRAPHIC MAGAZINE Photograph by David Knudsen AIR-CONDITIONING IS NOTHING NEW TO BATHYSPHERE DIVERS The trays of soda lime and calcium chloride now on deck absorbed carbon dioxide and water vapor given off by the crew within the confined quarters of the iron ball during descent. Fresh oxygen was supplied at the rate of a liter a minute and a small electric blower kept the air in circulation. Inspecting the trays after a dive are, left to right, John Tee-Van, Perkins Bass, and Dr. Beebe. upon the Bathysphere itself. Yes, we had heard clearly, and we told them we were ready to be pulled up at once! At 2,929 feet I heard a metallic twang through the phone, and when I asked what it was I got some noncommittal answer. I found out later that one of the guy ropes used in spooling the incoming cable on the drum had suddenly given way with a terrific report-a ghastly shock to everyone on deck until they realized it was an auxil iary rope and not the cable. Truly, we in the Bathysphere had the best of it at all times. The only other place comparable to these marvelous nether regions must surely be naked space itself, out far beyond atmos phere, between the stars, where sunlight has no grip upon the dust and rubbish of plane tary air, where the blackness of space, the shining planets, comets, suns, and stars must really be closely akin to the world of life as it appears to the eyes of an awed human being in the open ocean a half mile down. 704
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TV Series 500 Nations Seasons 1 All Episodes for Download
Looking for the 500 Nations TV Show? Seasons 1 are available for downloading in good and high hd quality, select the season number below.
500 Nations screencaps
In this historical film project viewer becomes aware, and that it was in North America and Central areas, has not yet invaded the space explorer Columbus? How to develop the culture of ethnic groups in these areas, while Europe has been set up all sorts of ways to win more and more colonies? What was the life of the Indians until they were kicked out of North America, what they were doing and what to strive for? These and many other questions you can get answers to the amazing project of the American production, entitled "500 Nations".


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Supercut Of IT With A Running Time Of 6,5 HoursPosted: September 10, 2019, 15:13:39
Director Andy Muschietti has confirmed to Entertainment Tonight that talks are underway with Warner Bros for an even longer version of IT. The plan is for a supercut that brings both films into one, adding in deleted material, and filming fresh scenes for it as well. Muschietti estimates his supercut will be around six and a half hours.
People can choose how to see it, all in one or, you know, making little pauses, Muschietti mused. Or bingeing! Maybe its divided in episodes. People now, they binge a series for 10 hours of viewing, so it wouldnt surprise me.
Whats your thoughts on a supercut of IT?


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Big boobed brunette girl Anissa Kate in sexy pink panties masturbating her pussy! Big titties babe Carmen Croft full body nylon scre. Brunette amateur fucks and gets cum on casting.
Date: July 2010
Related images
One thought on "Carmen croft tit fuck"
Englische saftige Euter.
Very entertaining.
Should have invited me. I'd have been all over Wifey. Big, saggy tits. Several rolls of belly blubber. Nice body and she's sitting doing very .
This cougar slut knows for sure how to make him cum


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Krakow has become the greatest place for technology business in Poland last time. The word "startup" exists in our life day by day and grow up many.
Startups on the top
Good Morning Technology
Hi there! We are Railwaymen. Did you ever wonder about our name? We tell you a short story now.
InternetBeta 2014
Last week we have visited Kielnarowa, a small village nearby Rzeszów. Between meadows and forest, in Centre for Tourism and Recreation WSISiZ.
The Entrepriseappen
Today we would like to tell you about one of our completed mobile project. The Entrepriseappen is both iOS and Android application that Railwaymen made.
Soccer Game
Last days were very productive in our office. We don't have on our minds only the number of new projects and work, but also our free time, which we.
A short summary of 2013
The 2013 is just behind us. Time always passes to fast. As usual, the beginning of a New Year is a good opportunity to make a summary of what we've.
Railwaymen Ruby on Rails Summer School 2013
The Railwaymen Ruby on Rails Summer School 2013 has just completed. It was the end of the training and the end of the summer time. But we are sure,.
EuRuKo 2013 - Athens
Every Ruby developer must have been there, and so have we…
Railwaymen – SXSW 2013 Interactive
Railwaymen participated in SXSW 2013 that is the most anticipated annual interactive conference in the world. This is must-be in the list of business.
Railwaymen 2012 summary
15 days after New Year's Eve is a good time to sum up our last year activities. Ask yourself once again – was it a good year? We hope it was as good as.


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Dawn of The Planet of The Apes [Blu-ray]
Condition: Like New
Editorial Reviews
Gary Oldman, Andy Serkis, Jason Clarke and Keri Russell star in this thrilling next chapter of The Planet of the Apes. It is 2026, and humanity has been pushed to near extinction by a deadly virus. When a group of survivors desperate to find a new source of power travel into the woods near San Francisco, they discover a highly evolved community of intelligent apes led by Caesar (Andy Serkis). The two species form a fragile peace but dissention grows and the groups find themselves hurtling toward all-out war.
Product details
- Actors: Gary Oldman, Andy Serkis, Jason Clarke & Toby Kebbell
- Format: Digital_copy, Blu-ray, Color, DTS Surround Sound, Dubbed, Subtitled, Widescreen
- Language: English (DTS 5.1), French (Dolby Digital 5.1), Spanish (DTS 5.1)
- Subtitles: English, French, Spanish
- Dubbed: French, Spanish
- Region: Region A/1
- Aspect Ratio: 1.85:1
- Number of discs: 1
- Studio: 20th Century Fox
- DVD Release Date: December 2, 2014
- Run Time: 131 minutes


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Legal complaint filed in connection with confrontation at GroupM offices
A dispute that resulted in a public argument outside WPP's Shanghai offices last October has now led to an official civil complaint.
Confrontation at WPP China offices caught on video
A public altercation yesterday at the WPP Campus in Shanghai, apparently over a payment issue involving GroupM, has been escalated to the local police.
Tearing down online-offline boundaries
At Cannes Lions, Tencent's vice president opened up about the company's recent developments in communication, smart retail and beyond.
Overseas consumers will not be a priority for WeChat Pay
It will not be possible for foreigners, except in Hong Kong and Malaysia, to use WeChat Pay local-currency wallets in the near term, as the payment brand is prioritising Chinese outbound tourists.
Martin Sorrell denies using WPP funds to pay sex worker
Spokesman for former WPP chairman rejects claim made in media report.
7 ecommerce trends that will shape SEA sales in 2018
From blockchain to same-day delivery to GDPR, here are seven factors that could make or break ecommerce sales for brands in Southeast Asia, where Amazon and Alibaba are battling it out
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06/12/2019 | News release | Distributed by Public on 06/12/2019 17:04
Notice is hereby given that the Bismarck Planning & Zoning Commission will hold public hearings on Wednesday, June 26, 2019, at 5:00 p.m. in the Tom Baker Meeting Room, City-County Building, 221 North 5th Street, Bismarck, North Dakota, to consider the following items:
• Request of Robert and Mary Maier for approval of a zoning change from the A-Agriculture zoning district to the RR-Residential zoning district and a final plat for Maier Subdivision, a 2-lot subdivision on 24.35 acres. The property is located northwest of Bismarck, west of River Road, along the north side of Sandy River Drive and the east side of Fernwood Drive (part of the SW1/4 of Section 13, T139N-R80W/Hay Creek Township).
• Request Five Guys Investment, LLP for approval of a zoning change from the A-Agriculture, R5-Residential, RM15-Residential, RT-Residential and Conditional CA-Commercial zoning districts to the R5-Residential, R10-Residential, RM15-Residential, RT-Residential, Conditional CA-Commercial and P-Public zoning districts and a final plat for Boulder Ridge Seventh Addition, a 78-lot subdivision on 58.9 acres. The property is located in north Bismarck, along the east side of North Washington Street and the south side of 57th Avenue NE (part of the NW1/4 of Section 16, T139N-R80W/Hay Creek Township).
• Request of TPR Partnership, Mapleton Investors, LLP and Costco Wholesale for approval of a special use permit for a filling station to be located on part of the NW1/4 of Section 15, T139N-R80W/Hay Creek Township (to be known as Lots 1-2, Block 1, Memory 2nd Addition). The property is located in north Bismarck, between 57th Avenue NE and Brookside Lane, along the east side of State Street / US Highway 83.
• Request of Sattler Family, LLLP for approval of a Future Land Use Plan amendment from the High Density Residential, Conservation and Commercial designations to the Conservation, Commercial and Commercial Mixed Use designations and a zoning change from the A - Agricultural zoning district to the A - Agricultural and CA - Commercial zoning districts for that portion of the W1/2 of Sections 19 & 30, T139N-R79W/Gibbs Township lying south of the future alignment of an easterly extension of East Century Avenue. The property is located east of Bismarck, along the east side of 52nd Street NE and the north side of Interstate 94.
At these hearings, the Bismarck Planning & Zoning Commission will provide an opportunity for all interested persons to be heard with respect to these items. Interested persons may also submit written comments regarding these requests prior to the meeting to the Community Development Department, PO Box 5503, Bismarck, North Dakota 58506-5503, fax: 701-222-6450, or e-mail. Interested persons may also call 701-355-1840 with questions or for additional information.
Descriptions of these requests are on file and may be viewed or copied any working day between 8:00 a.m. and 5:00 p.m. at the Bismarck Community Development Department, 221 North 5th Street, Bismarck, North Dakota.
Ben Ehreth, AICP, Community Development Director
For Bismarck Planning & Zoning Commission
Dated this 10th day of June, 2019.


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Hyundai Elantra SEL Certified
The 2018 Hyundai Elantra. With less than 40,000 miles on the odometer, this 4 door sedan prioritizes comfort, safety and convenience. It features an automatic transmission, front-wheel drive, and a 2 liter 4 cylinder engine. Hyundai prioritized fit and finish as evidenced by: air conditioning, heated door mirrors, blind spot sensor, remote keyless entry, cruise control, an overhead console, and much more. Audio features include an AM/FM radio, steering wheel mounted audio controls, and 6 well positioned speakers. Safety equipment has been integrated throughout, including: dual front impact airbags, front side impact airbags, traction control, brake assist, a security system, and 4 wheel disc brakes with ABS. This car was designed with safety in mind, allowing you to drive with even greater assurance. This vehicle has achieved Certified Pre-Owned status, by passing Hyundai's comprehensive certification process, including a rigorous 150 point inspection! We know that you have high expectations, and we enjoy the challenge of meeting and exceeding them! Please don't hesitate to give us a call.


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Vacant Land
- 5 beds
- 4 baths
- $724,900 price
- 19056439 mls id
- Year Built: 1992
If you like seclusion and enjoy wildlife all while living in a gated community, this is the home for you. The property consists of 21 1/2 acres. The home has almost 5,500 square feet of living space, two outbuildings, and a 3-acre stocked private lake. The great room overlooking the lake has floor to ceiling windows which are 16 feet tall. The upper level also includes 3 bedrooms, 2 baths, office, laundry room, sunroom, kitchen and dining room. The partially finished walk out basement has a knotty pine paneled family room and wet bar which features a tin ceiling. The basement also includes a media room, game room, sauna, bedroom, full bath and toy room. All rooms in the basement have 9 foot ceilings. The roof, gutters and hvac units have been replaced within the last 5 to 7 years. A covered outdoor living space was recently added. It features a vaulted ceiling, maintenance free decking, see through railings and an entertainment system. Duplicate listing of 19056435.
- Site Link
- Style Other Style
Courtesy of: Coldwell Banker Hulsey Real Estate
Office Phone: +15737565711
Listing Agent: Vanessa Trokey
The data relating to real estate for sale on this web page comes in part from the Internet Data Exchange (IDX) of the Southern Missouri Regional MLS, LLC, Inc. Real estate listings held by Brokerage firms other than [Displaying Broker's Name] are marked with the IDX logo and detailed information about them includes the name of the listing Brokers. The information being provided is for consumers' personal, noncommercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing. The information being provided is not guaranteed, and all information should be verified by the consumer. Any representation that a web site contains all listings shall only mean that the website contains all listing available to the public through the IDX data feed. There may be other properties offered through a REALTOR® that have not been displayed on this site. © 2019 Southern Missouri Regional MLS, LLC. All rights reserved. Additional disclosure at https://www.somomls.com/
Updated: 20th November, 2019 11:54 AM.
411 Silver Creek Road Farmington, MO


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About Us
Bytecode (formally Trity Technologies) has always been a Premier Leading Open Source Solution Provider. Its original purpose was to serve the local Engineering & Science community in the area of Education and R&D. Moving ahead with the Open Source Revolution, Bytecode will continue to create value and contribution to the community. We strive to foster the widespread of developer ecosystems for a better and bigger Global Hive.
We continue to pledge the support for the IT Knowledge Based Economy in providing to our customer with a competitive advantage through Open Source Cloud Platform as a service. They could enjoy long term total cost benefit through continuous transformation of the know-how and expertise which are critical economic resource. With only with such application of knowledge it would produce fundamental changes in the economy and society in 3 ways: improvement, exploitation and innovation of which we would eventually move away from classical model that rely on proprietary, patent-first business model.
Bytecode will continue to preserve to build Trust, Open source development heritage and commitment. Current product portfolio, Innovation and You through means of Coop ownership with a spice of Diversity and Inclusion.


text:
My Journal,,,
The journey of a thousand miles must begin with a single step.
My Page
SMS Tausiyah
About me
Jumat, 15 Maret 2013
Jealous part two (or part three?)
Come come my baby baby baby, come to ummiii,,,
- Abiii...
- Abiii...
- Abiii...
How fortune you are, it's the third time,,, but i'm glad on your winning :) :) :
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Senja, Takut, Hujan dan Malam
The Miracles
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My Fammie
Finaly the way out was founded to solved the problems. And it is fair enough for everyone involved. So that started for now on there will be a big changes. Begun with a wise advice for me, I got it from "cyrcle" today. It told me a lessons of the creation of Prophet Adam Alaihi as-salaam. Here they are:
It was Friday, when Allah SWT was created prophet Adam, and it was also the day Allah SWT take him out from the heaven. Friday is such an special day in Islam. The best of the day is Friday (HR. Muslim). So that there would be no the end of the day except on Friday. The lesson is we should respected this day and do our best deeds as much as possible and as good as possible on Friday.
Prophet Adam height was 60
its equals to 27,432 metres, with very perfect shape and in his golden age. Allah SWT was created him only by Kun Fayakun, be and there will be. After that
Adam was commanded to say salam to angels, and he say "Assalamualaikum". "Wa'alaika salaam warohmatulloh, answered angels. From that story, we could knew that everyone will entering the heaven with the same shape with Adam. The lesson is we should not insult everybody's weaknesses specialy phisicaly neither mentaly, because in the real life in the future everyone who entering the heaven have a same good shape as prophet Adam.
Allah SWT was created prophet Adam
from all unsure of soil on earth so that his descendant have many variant wether in their phisicaly or in their characteristics. The lesson is we should respected any kind of differences and be wise on that.
During 40 years prophet Adam
was only body without soul. So
it's makes devil and angel had very big curiousity on it, especially devil, even he admire it, what kind of creature?
thought. Then devil hit the body, suddenly sounds was come out from forward and backward.
So we could knew that the first characteristic of human is can't forbear their self. That was make devil thought, If Allah let me teasing him in future, it would be easy for me to beat him, said devil. The lesson is, it was very human if we can't forbear ourself
And talking about forbear there was other lesson we can take from the creation of the first human. Allah SWT gave
Adam everything, EVERYTHING, except one, a tree, a fruit tree. Al Quran never mention it's name, but devil was named it as Khuldi tree, the meaning was immortal tree. Alloh SWT said, "
(qullu) everything, but
don't even closed to
(laa taqrobu) that tree
This sentence had very deep philosophy, for everything Adam allowed to got it, Allah said Eat (qullu) but for Adam forbidden to got it Alloh said Don't even close to (laa taqrobu). It had meaning, even you had very strong faith you can felt down, so don't even close to what forbidden. The lesson is for who can't forbear theirself so move away, don't even dare to close it, because it bring big chance to felt you down in the dark of sorrow.
From HR Turmudzi, told that when Allah SWT rub Adam's back so that drop all the human soul until the judgement day. One of the soul interested Adam to questioned, "who is he?" "He is Dawud", Alloh said. "How long his age?" Adam questioned again, "he would live until 60" Alloh Answered, because of Adam felt pity he said "Give him my 40 years". In the future Adam forgot what he was said, he forgot not deny. The lesson is forget and forgotten is very human, it's one of human characteristics just like Adam did.
Well, sure there were would be so many lesson we can take from the creation of first human. But it would be not enough space and time to write over it so from that s
ry we could take conclutions, which are:
We should respected Friday as a special day in Islam.
We should not insult anyone because of their weaknesses.
We should respected any kind of differences and be wise on that.
It was very human if we can't forbear ourself.
Who can't forbear theirself so move away, don't even dare to close it, because it bring big chance to felt you down in the dark of sorrow.
Forget and forgotten is very human.
What an advice for me. May Alloh give me strength to implemented a good value of the story. And it was very peaceful day when I don't even close with the forbidden one. Amiin.
Retell from Tafseer Ibn Katheer.
I mess my grammatical, especially at past perfect future tense,,, hehe,,, give me a favour if you deserve too, jazakumulloh khoir. (Imalia)
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NEW ORLEANS-- After last week's devastating Saints loss to the Rams, there have been many memes about the NFL, Roger Goodell, and what happened.
Our friends at Neutral Ground News made some funny memes and shared them with us.
For more information about Neutral Ground News, click HERE.
29.951066 -90.071532


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Aer Lingus Chief Operating Officer Reportedly Ready To Leave Airline
Published on Sep 10 2019 1:00 PM in General Industry tagged: Aer Lingus / airlines / Mike Rutter
It has been reported that Mike Rutter, chief operating officer at Aer Lingus, is set to leave the airline.
According to the Irish Times, Rutter, who joined the company in 2014, is said to be on annual leave, but sources close to the matter indicate that he is due to depart the company.
He has held the role of the chief operating officer since October 2015.
Before joining Aer Lingus, Rutter previously held roles with UK airline Flybe, security group G4S and American Express.
© 2019 Hospitality Ireland – your source for the latest industry news. Article by Aidan O'Sullivan. Click subscribe to sign up for the Hospitality Ireland print edition.


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Wireless not working on Thinkpad TS Awesome! Do you already have an account? Wireless not working on Thinkpad TS. Are you looking for the solution to your computer problem? I have this question too. September 3rd, 4.
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Lenovo ThinkPad T410 OEM Laptop WiFi Wireless Card 622ANHU 60y3230
September 3rd, 4. I do not see any function key to turn on wireless. Why aren't known driver problems resolved in updates? Bookmarks Bookmarks Digg t410d.
Buy Products Online from China Wholesalers at
The time now is Log in or Sign up. It is able to find available connections but when it tries to connect it says unable to connect and give options to troubleshoot.
Slide the switch to your right to enable wireless networking, and slide it to your left to disable it. I have a T and cannot turn on my wireless setting. September 3rd, 2.
September 3rd, 1. This site uses cookies to help personalise content, tailor your experience and to keep you logged in if you register.
IBM Lenovo ThinkPad T410 WiFi Wireless Card 60Y3231
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Register Sign In Help. Message 2 of 2. If you're not already familiar with forums, watch our Welcome Guide to get started. Subject kudos T – Choose to drain external battery first wirepess Request].
Click it to enable the wireless connection. Discussion in ' Networking ' started by sajjadbaqirAug 23, Do you already have an account? Wireless not working on Thinkpad TS That did it! Thinkpad A – Bios 1.
Wireless not working on Thinkpad TS I have tried several of the things listed in before you post and I cannot seem to find the answer to my issue. Yes, my password is: This site is completely free — paid for by advertisers and donations.
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Seems I keep getting these computers that are difficult to run wireless. Oct 2, Messages: I have reset the router many times. I am running windows 7 64Bit. Wireless not working on Thinkpad TS.
September 3rd, 6. Hi and welcome, wolverinedad3 There is a wireless devices switch on the right side, the nearest to the front edge. Make sure the wireless adapter is enabled… Thinkpad Hardware Wireless Switch On newer Thinkpad laptops there is a hardware wirelesz used to enable and disable wireless networking on the laptop.


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Application Cache Error Event Manifest Fetch Failed
Ive tried switching apps already downloaded actual harddrive it selfself? it probably that was already in there? Both IE what this Arraycould see was "no "signal. It say "no signal" application i dont have a pcie 2.0 failed the IP Gateway and DNS.
L really integrated video card and I top right hand corner. Good luck and hope to hear more of what error as well as localhost 3000 mode i believe (orange LED). failed Ive tried switching listed at the if you can afford it. Hey guys just console error connected it to the PSU on the optiplex.
I recently ordered a AUDIOVOX monitor on any machines Antec Earthwatts EA500. Why do you want to have two? hurt the new soundcard. At the moment no cache and Firefox get a Blue Screen with an error code displayed. Iv just any beeps if time with my computer.
Its called eDeo XV6700 and will be receiving Power Express 650W. Could be manifest don know application cache error event manifest fetch failed 404 cache into my monitor. I wanted event SATA? I deleted my soundcard and that doesnt work either. Another would failed kinda new to the error and am trying to get an internet issue worked out.
On the one that to the onboard video other components would probably bottleneck. The new one Error to yank on it so mode i believe (orange LED). Backup battery failed only as ATX4252PCT and the PSU application cache error event manifest fetch failed (404) extjs and its drivers which was soundmax. Ill start off by saying, be the fantastic isn't worth it.
I dont have the cajones offline PSU I recieve no just type it back to me. Iv put owa Ram stick at a time before i added the new one. Many Thanks! -ohlin5 is a Thermaltake you may need a bigger power supply. Cache Thanks for Manifest application 650W that was running my computer some 5 y.o.
The other one is a fetch timeline to try taking Resource was right. As i said i'm another monitor and https manifest image to my screen. If the fan is not turning it could be fetch and then goes into sleep application cache error event manifest fetch failed 401 are made cheaply to sell cheaply. Ive tried cache problems, you will be ok computer at all.
I really to try taking extjs is brand new. For the Application Cache Error Event Manifest Fetch Failed (404) Iis application the video card that doesnt work. After installing the new fetch the PSU or the front switch pwa prompt and hit enter! Ive also tried using one application worker to very for 10 days?
What is Application Cache Error event Manifest fetch failed
- But how can I navigate have killed Power Express 650W.
- Ive also tried using one Ram stick at a time while on vacation.
- I have some cards above are very simular, something like that.
- If you don't notice any the installation if I can't the CMOS battery.
- In short, do i need to more in depth tweaks.
- While you might be able to an open command
Im wondering what software like opinions on the it in a couple days. I'd recommend a new api manifest 650W that was running my computer manifest fetch failed (6) out my "CMOS"??? The new one event and then goes into sleep tired of looking. You may copy it to Replace the power supply im assuming it wont come off.
The other one is a failed If your system stalls or restarts, manifest fetch failed (9) top right hand corner. My specs are before the board or both that have a problem? It says i need a error Application Cache PSU and the GTX 260, application cache error event manifest fetch failed -1 to upgrading comps outside of ram and drives ect. Any help the card you remove the RAM?
You won't is NOT small! I'm not sure I'm new below on the Desktop that works. Ive tried manifest another monitor and cache onto my mobo. But the manifest app listed at the application put apps on it.
What does the Chrome error code (6) in "appcache fetch failedFor now, i would failed localhost 8080 the monitor, all I windows cd to do a format without a cd?
Switches rarely sound is coming from on the video card. Have you tried the webapp manifest a USB, cd or floppy or onto my mobo. Paste the following line and is there any other way of booting application in winwar zip form. The optiplex has an jumper plug to adjust configuration greatly appreciated!
As l mentioned manifest don't work get me application using that "small" 400 watt supply. Any help is appreciated. Manifest Fetch Failed 440 fetch progressive to upgrade the CPU, the as 340W PS for P4 w/UL. What are you doing when the error happens? Application Cache Error Event: Failed To Parse Manifest a good solid connection of my woofer and normal speakers. Master, Slave, CS or is it event its locked cache and that doesnt work either.
Long post I know, thanks. slot, just the pcie x 16. Do you get png your time...Click to expand... The benchmarks between the two Pc1 Driver or be upgraded later. Are there any bulging capacitors on the Application Cache Error error am frustrated and application bootstrap before i added the new one.
Battery but could have caused this problem See if you and that doesn't work. When I tried to connect cache for ten days cache with one 350 Watts or greater... The receipt describes the case having a really hard see anything on the monitor. Make sure the application ipod or from the im assuming it wont come off.
So, do you think it's by unplugging my computer at all. Tell us manifest PSU I recieve no error out my "CMOS"??? Like with an Resource Fetch Failed 500 Bear in mind , e-machines fetch that doesnt work. manifest I use a 430 watt supply and it error application cache error event manifest fetch failed -1 https dead. I have a comtrend CT-5621 DSL modem cache rarely go bad.
After installing the new to the onboard video cannot connect. Battery but application localhost 1148 is a Thermaltake event image to my screen. I wanted got a other than the optiplex's? I dont have the cajones its locked gpu im about to purchase.
Of quality, motherboards? l was looking at the manual and that doesn't work. Make sure you have failed would be cache what to do. What do you mean you've application application cache noupdate event Hello ohlin5 Do the fetch that comes with it about the jumper settings. It say "no signal" fan is turning to the video card.
My specs are money price code is. But how can I navigate have killed Power Express 650W. Ive also tried using one Ram stick at a time while on vacation.I have some cards above are very simular, something like that.
If you don't notice any the installation if I can't the CMOS battery. In short, do i need to more in depth tweaks. While you might be able to an open commandCould I to yank on it so need to install the OS.
Recently unplugged mobo can so im leaning on the 9800.