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|Cancer Research
|Clinical Cancer Research
|Cancer Epidemiology Biomarkers & Prevention
|Molecular Cancer Therapeutics
|Molecular Cancer Research
|Cell Growth & Differentiation
Departments of Microbiology/Immunology [C. B., G. W. K.], Medicine [J. L., G. W. K.], and Radiation Oncology [P. D.], Medical College of Virginia of Virginia Commonwealth University and McGuire Veterans Affairs Medical Center, Richmond, Virginia 23249
Materials and Methods
Materials and Methods
The c-kit gene encodes a transmembrane tyrosine kinase highly related to the receptors for PDGF and CSF-1 (12) . Whereas Kit is capable of activating multiple signal transduction pathways (13) , the pathways responsible for the proliferation of SCLC cells have not been defined. Evidence obtained using microinjection of inhibitory antibodies or DN expression constructs indicates that both the PDGFR and the CSF-1R require active Src family kinases to initiate DNA synthesis in fibroblasts (14 , 15) . However, using the alternative approach of mutating the Src binding sites on the PDGFR, other investigators have concluded that activation of Src kinases is not necessary for PDGF-induced proliferation (16, 17, 18) . We hypothesized that activation of Src family kinases could be involved in SCF-mediated proliferation of SCLC based on the fact that ectopic expression of Lck occurs in some SCLC cell lines (19) that also express high levels of Kit and on the observation that Src kinases are activated in SCLC (20) . We recently demonstrated that, in the H526 cell line, the proliferation of which can be stimulated by exogenous SCF, SCF induced both the physical association of Lck and Kit as well as an activation of Lck that was equivalent to that seen after the stimulation of T cells (21) . The time course of Lck activation paralleled that of MAPK activation, which suggested that Lck activation plays a role in early events in Kit signal transduction; the present study explores that role.
Materials and Methods
Materials and Methods
Other groups have taken the alternative approach of mutating the juxtamembrane tyrosines of the class-III receptors required for Src binding rather than directly inhibiting Src kinases (16, 17, 18) . Although they universally have failed to define a role for Src kinases in proliferative signal transduction mediated by the PDGFR using this approach, cells expressing the mutant receptor did show decreased phosphorylation of multiple downstream SH2-containing signaling molecules and an inability to efficiently activate ERK 1 and 2 at high ligand concentrations (16) . This last observation is consistent with our findings. Timokhina et al. (39) took a similar approach, demonstrating that the Y567F mutation, which abolished Fyn binding to murine Kit, had no effect on SCF-mediated proliferation of bone marrow-derived mast cells. More recently Lennartsson et al. (40) have demonstrated that the homologous Y568F mutation in human Kit blocked SCF-mediated Src kinase and Ras/MAPK activation in transfected PAE cells, in excellent agreement with our findings. They localized the requirement for Src kinases to a step proximal to, or at the level of, Shc phosphorylation. Interestingly, they observed that the Y568F mutation did not significantly affect SCF-mediated proliferation, but the Y568F, Y570F double mutant resulted in a severely impaired proliferative response.
The inconsistencies between studies regarding the requirement for Src kinase activation for activation of MAPK and proliferation by class-III RTKs are likely attributable to numerous factors. Some discrepancies are likely to be attributed to potential functional differences between the individual receptors. Undoubtedly, the cellular background in which these receptors are expressed plays a role, as evidenced by our observation that the lack of a requirement for MAPK activation for proliferation is in part dependent on the loss of functional Rb in SCLC cells. Most importantly, however, the technique used to interfere with Src kinase activity likely affects the outcome and interpretation of the data. Whereas the juxtamembrane tyrosines of Kit are clearly important for binding Src kinases, they also constitute the binding sites for the SHP phosphatases (41) and the CHK kinase (42) , which suggests that their mutation could cause pleiotropic effects. On the other hand, microinjected antibodies or DN proteins could interfere with other critical Src functions unrelated to RTK signaling. It has been suggested that the discrepancy between studies as to the requirement for Src kinases to initiate proliferation by class-III RTKs may relate to the unperturbed basal level of Src activity in cells containing the mutant receptors (16) . Our studies cannot distinguish between a requirement for Src kinase activation versus basal Src kinase activity, because it is likely that both are affected by PP1 and DN Lck expression. It is important, however, to point out that activation of Lck alone is insufficient for MAPK activation in H526 cells, because overexpression of Lck or expression of the constitutively activated mutant Y505F Lck by itself did not result in significant MAPK activation in the absence of SCF (Fig. 4 and 5) . This observation could indicate a requirement for Lck activation in proximity to the activated receptor for efficient MAPK activation, because numerous studies discussed above have demonstrated a ligand-induced association between the receptor and Src family kinases. Additional experimentation will be required to elucidate the precise mechanism by which Lck promotes MAPK activation after activation of Kit in SCLC cells.
As a by-product of the above studies we have determined that PP1 is not an absolutely specific inhibitor of Src family kinases but also efficiently inhibits Kit and potentially other class-III RTKs. During initial studies of the role of Lck in Kit signal transduction we performed an IP-kinase assay using Kit from PP1-treated cells to determine the effect of the drug on Kit activity (21) ; other investigators have also performed similar assays (43 , 44) . We chose this activity assay because it theoretically would avoid detecting potential tyrosine phosphorylation of Kit by Lck, which could complicate direct analysis of receptor tyrosine autophosphorylation. However, in the present study, when we undertook such an analysis to confirm the results of the IP-kinase assay, we found a 9095% inhibition of Kit tyrosine phosphorylation at 510 µM PP1, in all likelihood too large to be accounted for by inhibition of Lck-mediated phosphorylation of the receptor. We confirmed direct inhibition of Kit activity by in vitro addition of PP1 to an IP-kinase assay. It appears that the IP-kinase assay using Kit from PP1-treated cells yielded misleading results, probably as a result of drug dissociation during the IP procedure. Thus, it is apparent that PP1 cannot be used as a probe for Src family kinase function when class-III RTK function may also be involved in the biological phenomenon being studied unless other independent confirmatory data are also obtained.
When these studies were initiated, their goal was to identify the signal transduction pathways involving Lck that are required for SCF-mediated SCLC growth (21) . Although the Ras-MAPK pathway initially seemed an excellent candidate based on its importance for the growth of other cell types and its sensitivity to Src kinase inhibition, attempts to confirm the requirement for activation of this pathway by directly inhibiting MEK failed. The lack of a requirement for MAPK activity for growth of several SCLC cell lines was initially puzzling, but a possible explanation for this result became apparent after trying to place it in the context of other genetic alterations in SCLC. The synthesis of the D-type cyclins, phosphorylation of Rb, and subsequent cell cycle progression have long been known to be responsive to growth factor stimulation (45) . However, recently several groups have linked the activity of cyclin D-dependent kinases directly to activation of the Ras-MAPK cascade in NIH 3T3 fibroblasts (46, 47, 48) . Ectopic expression of an activated form of MEK 1 could enhance cyclin D1 expression and drive the cells through S-phase if the p27Kip1 inhibitor were neutralized (47) . Conversely, DN forms of Ras and Raf could block expression of cyclin D1 and subsequent cell cycle progression in response to serum stimulation, a situation that could be rescued by ectopic expression of cyclin D1 (46) . Finally, in cells whose expression of cyclin D1 was blocked by DN Ras, structural inactivation of Rb allowed cell cycle progression (48) . This last observation was particularly revealing because it suggested that the almost universal loss of Rb function in SCLC (49 , 50) could be responsible for the observed insensitivity to PD 98059. Expression of Rb in H526 cells resulted in a partial restoration of sensitivity to the MEK inhibitor, which supports this theory. We believe that one potential reason for the incomplete restoration of sensitivity could be attributable to the effects of high levels of N-Myc expression in this cell line; overexpression of N-Myc by itself has been shown to drive cells through S phase (30) . Thus, the insensitivity to MAPK inhibition demonstrated by SCLC cells in the absence of Rb expression confirms the observations made in the 3T3 model system in a common human tumor. Although it is clear that, in an established tumor cell line, loss of functional Rb plays a dominant role in the loss of G1-S phase checkpoint regulation, it is possible that activation of the MAPK pathway could play a more important role during the pathogenesis of the tumor before the inactivation of Rb. More practically, our observations suggest that therapeutic strategies aimed at inhibition of the MAPK cascade may not be effective in tumors with a structurally inactivated Rb gene. In addition, our observations suggest the existence of one or more alternative signal transduction pathways that must be responsible for mediating SCF-stimulated growth of SCLC. Identification of these pathways, along with the precise mechanism by which Lck assists Kit in activating MAPK, will be the subject of future investigation.
|Materials and Methods
Materials and Methods
Growth Assays.
Cell growth was measured using the MTT (Sigma) colorimetric dye reduction method, an assay shown to correlate very well with viable SCLC cell number under the conditions used (51) . Duplicate plates containing eight replicate wells per assay condition were seeded at a density of 1 x 104 cells in 0.1 ml of medium, and data were expressed as the change in absorbance at 540 nm over 72 h, relative to initial values obtained 3 h after plating. Tritiated thymidine incorporation in eight replicate microplate wells containing 1 x 104 cells each was determined after a 3-h incubation in medium containing 1 µCi/ml [3 H]thymidine (NEN, Boston, MA). The cells were collected on GFC filter plates using an automated cell harvester (Packard Instruments, Meriden, CT), were lysed and extensively washed with distilled water, and assayed by liquid scintillation counting.
Derivation of Cells Capable of Inducible Lck Expression.
H526 cells were transfected with the pTet-On plasmid encoding the reverse Tet repressor (Clontech, Palo Alto, CA) using electroporation parameters described previously (52) . Subclones were isolated by limiting dilution during selection with 1 mg/ml G418 (Life Technologies). The drug-resistant subclones were transiently transfected with the pTRE-Luc luciferase expression plasmid, and the IC4 subclone that had the lowest basal luciferase activity and the highest degree of doxycycline-induced activity was selected as the recipient for secondary transfections. An EcoRI fragment containing the entire murine lck cDNA (ATCC 63228) was cloned into the pTRE expression vector (Clontech) to produce the pTRE-WT Lck expression vector. The K273R and Y505F mutations were introduced into this vector using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA) and the following mutagenic oligonucleotides to produce the pTRE-DN Lck expression vector: K273R, 5'-CACGAAGGTGGCGGTGAGATCTCTGAAACAAGGGAGCATC-3' and its complement; Y505F, 5'-CACAGAGGGCCAGTTCCAGCCCCAGCCTTGATATCCCTTTCG-3' and its complement. The Y505F mutation was also singly introduced into the WT vector. In addition, to enhance translational efficiency, all of the three out-of-frame ATGs in the 5' untranslated region (53) were mutated using the same technique. All of the mutations were confirmed by sequencing. The Lck expression vectors were cotransfected with pSV2-Hygro into H526-IC4 cells, and subclones were selected by limiting dilution during culture in 600 µg/ml hygromycin (Calbiochem). The drug resistant subclones were assayed for doxycycline-inducible expression of Lck using Western blotting. The predicted kinase activities of the mutant Lck proteins were confirmed by IP kinase assays as described previously (21) . Lck expression was induced for 24 h using 2 µg/ml doxycycline (Sigma) before growth factor stimulation and MAPK assay.
IP, Immune Complex Kinase Assay, and Western Blotting.
H526 cells were lysed in a buffer containing 50 mM HEPES (pH 7.5), 150 mM NaCl, 1% NP40, 1.5 mM MgCl2, 1 mM EGTA, 10% glycerol, 0.2 mM NaVO4, 100 µg/ml phenylmethylsulfonyl fluoride, 1 µg/ml aprotinin, and 10 µg/ml leupeptin using a Dounce homogenizer with a tight-fitting pestle; protein concentrations were determined by BCA assay (Pierce, Rockford, IL). The lysate, containing 11.5 mg of protein, was centrifuged for 10 min at 10,000 x g to obtain a soluble postnuclear supernatant. IP was initiated by the addition of 10 µg of monoclonal anti-Kit antibody (K45; NeoMarkers, Fremont, CA), followed by incubation for 2 h at 4oC and by an additional 2 h in the presence of Protein A+G agarose. The IP was washed four times in lysis buffer and then once in PBS. The pellet was aspirated to dryness and 30 µl of kinase buffer (20 mM PIPES and 10 mM MnCl2) containing 10 µCi of [P32]ATP was added. The kinase reaction was carried out for 10 min at room temperature and was terminated by the addition of an equal volume of 2x SDS sample-loading buffer; the reaction was then resolved on a 10% polyacrylamide gel and was analyzed by autoradiography. Western blotting was performed using standard procedures, with detection using the ECL chemiluminescent system (Amersham, Arlington Heights, IL) and visualization using a Fuji cooled CCD camera and the Aida 2.0 software package (Raytest Inc., New Castle, DE). Staining was accomplished using the following antibodies: anti-Kit, 3D6 monoclonal (Boehringer-Mannheim); antiphosphotyrosine, PY20 monoclonal (Transduction Labs, Lexington, KY); affinity-purified polyclonal antiactive MAPK (Promega, Madison, WI); polyclonal anti-ERK 1 CT (pan-ERK; UBI, Saranac Lake, NY); polyclonal anti-Lck (PharMingen, San Diego, CA); monoclonal anti-Rb (IF8; Santa Cruz Biotechnology, Santa Cruz, CA).
MAPK Assay.
The ability of immunoprecipitated MAPK to directly phosphorylate myelin basic protein was determined as described previously (54) .
Rb Phosphorylation Analysis.
H526 cells expressing doxycycline-inducible Rb protein were derived exactly as described for WT Lck-expressing cells using a full-length WT Rb cDNA cloned into the pTRE vector. Subclones capable of doxycycline-regulated expression were induced for 24 h in complete medium containing 2 µg/ml doxycycline; were placed in serum-free, phosphate-free medium containing 1 mCi/ml [32P]Pi (NEN) for 6 h; and then were stimulated with 100 ng/ml SCF after a 30-min preincubation in DMSO or 80 µM PD98059. The incubation was continued for 12 h, the cells were lysed in triple-detergent buffer (21) , and cleared lysates containing 0.5 mg of protein were used for IP using a monoclonal anti-Rb antibody (Santa Cruz). The immunoprecipitates were electrophoretically resolved and analyzed by autoradiography, phosphorimage analysis, and Western blotting. Staining with phospho-specific anti-Rb antibodies was accomplished using the PhosphoPlus antibody kit (New England Biolabs, Beverly, MA).
1 This work was supported by a Merit Review Award from the Department of Veterans Affairs (to G. W. K.) and by grants from the American Cancer Society (IN-105V) and the V Foundation (to P. D.).
2 To whom requests for reprints should be addressed, at Richmond Veterans Affairs Medical Center (111K), 1201 Broad Rock Boulevard, Richmond VA 23249. Phone: (804) 675-5446; Fax: (804) 675-5447; E-mail: GKRYSTAL{at}HSC.VCU.edu
3 The abbreviations used are: SCLC, small cell lung cancer; SCF, stem cell factor; DN, dominant negative; IGF-1, insulin-like growth factor-1; PDGF, platelet-derived growth factor; PDGFR, PDGF receptor; CSF, colony-stimulating factor; CSF-1R, CSF-1 receptor; IP, immunoprecipitation; MTT, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide; Rb, retinoblastoma (tumor suppressor); MAP, mitogen-activated protein; ERK, extracellular signalregulated kinase; MEK, MAP/ERK kinase; RTK, receptor tyrosine kinase; WT, wild type.
Received for publication 1/24/00. Revision received 5/10/00. Accepted for publication 5/10/00.
Materials and Methods
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|Cancer Research
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Franklin Pierce Lake Nh
Aug 15, 2013. When people come to visit, I like to take them to see the "Haunted Camp" here on Lake Pierce in Antrim, NH. The "camp", which is the.
Base map data from NH GRANIT at Earth Systems Research Center (UNH) and. Pond. White. Pond. Franklin. Pierce. Lake. R ive r. W. Re. Deering. Reservoir.
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Franklin Pierce was the 14th president and served between 1853 and 1857. By Simonton's estimates, Pierce had an IQ of 141. After graduating from Bowdoin College, Pierce was elected to the New.
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Gilford is a town in Belknap County, New Hampshire, United States.The population was 7,126 at the 2010 census. Situated on Lake Winnipesaukee, Gilford is home to Governors Island, Ellacoya State Beach, Belknap Mountain State Forest, Gunstock Mountain Ski Resort, and Bank of New Hampshire Pavilion at Meadowbrook, a seasonal outdoor concert venue.The lakeside village of Glendale lies.
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With the incidence of myopia (nearsightedness) rising worldwide,
researchers recently sought to look for possible causes, which remain
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The complex mother daughter relationship
The Complexity of the Mother-Daughter relationship
Aug 16, In the opening of Greta Gerwig's Lady Bird, Saoirse Ronan's teenage character, Christine, and her careworn mother. Marion, listen tearfully to a. Apr 25, The mother-daughter relationship is a very special one, but according to the author, it is rather complex with "reports that 90 percent of mothers. Mother-daughter relationships are complex and diverse. Some mothers and daughters are best friends. Others talk once a week. Some see each other weekly;.
Economic, social, and religious aspects prompt this action. In ancient Greece infanticide was considered barbaric. No one really wants to outright kill a child. That being said, if a child was unwanted or could not be cared for by the parent they practiced exposure instead.6 Types of Unhealthy Father Daughter Relationships
This practice essentially abandoned the child on the roadside and at the mercy of weather, nature, or a merciful passerby. This was not considered murder because there was a chance someone would rescue it. The drastic change in the socio-status may leave a new mother feeling bitter, resentful and sincerely guilty for feeling that way.
Far from committing infanticide those feelings potentially cause an emotional and sometimes physical rift the child can neither recognize nor fully understand until perhaps, she becomes a mother herself.
Time will tell In their younger years, the first-born daughter may feel rejected by her mother. There is a sense of rivalry in some invisible competition.
Understand these are worst-case scenarios and theories for a complex relationship between mother and first child. This is not to assume all mothers and first daughters are so negatively affected.
My Perspective on the Complex Mother and Eldest Daughter Relationship | WeHaveKids
Once the initial shock of role changes are overcome; once a daughter becomes a mother, there is a certain beauty to passing the torch from one generation of women to another. Once a daughter, now a mother is elevated into a new peer group where her mother can now be her friend. Once the dust is settled from our childhood perhaps then there is a sense of equanimity. Only time can tell. They range from lack of confidence and trust, through to over-sensitivity and self blame.
And the replication of the insecure attachment, a powerful tutor in the unreliability and danger of love, plays havoc with wellbeing. We know that part of growing up is finding your own identity - and in infancy and childhood that first glimpse of the self is in the mirror of the mother's face. Deep self-criticism often comes about as a result of a child's internalisation of the harsh and abusive criticisms of a parent.
And daughters who did not feel love and verbally aggressive or emotionally absent mothers, struggle terribly with self-criticism, and finding a way to regulate negative emotions.
Are mothers more critical of their daughters than their sons?
Yes, says a 2,strong survey by UK parenting website, Netmums. More than half the mothers interviewed said that they had formed a stronger bond with their sons.
The Final Call
Mothers were more likely to describe little girls as "stroppy" and "serious", and sons as "cheeky" and "loving". As Susie Orbach, author and psychotherapist, currently appearing at The Writers Festival in Aucklandsaid in a Guardian interview: As though they're teaching them to still their pain or their own distress - it's the way women have been brought up". This corrosive self-criticism is the mental habit of attributing bad outcomes or situations failing a test, a relationship unravelling, not getting a job to generalised fixed characteristics about the self - rather than to a series of cause and effects.
It can also result in finding a partner, who will display the same attachment characteristics as the earlier relationship, creating the same pattern of self-blame and insecurity.


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I've had different people wonder how to go back and read Cody's story, right from the beginning (which was the first week in November, 2008-- I would have to check with Cody or Scott Smith for the exact date.) To facilitate easier reading, I've changed the archive format over on the right hand side. It now is in chronological order, starting with November. Just click on the date to start reading! Once you read that day's post, you can click on the next date at the bottom of the page. This format isn't as attractive, but it is easier to use! Hope that helps folks out!
P.S. Scott confirms it was November 3rd that they started out.
Friday, February 20, 2009
Changes to Blog Format
Posted by Evan and Clover and Co. at 6:46 PM 1 comment:
"Cody's Legs Must Hurt" Pictures
Cody texted me today that he had crossed the Edmund Pettus bridge (scene of a bloody attack against civil rights protesters marching from Selma to Montgomery). He is now doing the 54 mile trek that the civil rights marchers eventually made. They only averaged 10 miles a day, due to their large numbers, so let's hope Cody does better mileage than that!
The pictures I'm posting tonight are pictures that made me think, "Ouch!" There was some major climbing there at one point, as well as daily trudging along.
Beautiful scenery, though!
The pictures I'm posting tonight are pictures that made me think, "Ouch!" There was some major climbing there at one point, as well as daily trudging along.
This sign had to be a little discouraging!
Beautiful scenery, though!
This is when he still had his stroller. I'm sure glad a semi didn't come around that curve!
Posted by Evan and Clover and Co. at 5:33 PM No comments:
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- Free WiFi
- Kitchen
- Pet Friendly
- Laundry
What's nearby
- Euralille
- 13 min walk
- Hospice Comtesse
- 14 min walk
- Casino Barriere Lille
- 15 min walk
- Lille (LIL-Lesquin)
- 10 min drive
Your dates need to occur after 2019-11-15. Try updating your dates to see prices for your trip.
About this area
La Madeleine
Located in La Madeleine, Appart'City Confort Lille - Euralille is in the suburbs and near a metro station. Palace of Fine Arts and Zenith Arena Concert Hall are cultural highlights, and some of the area's notable landmarks include Old Stock Exchange and Rihour Square. Looking to enjoy an event or a game while in town? See what's happening at Pierre Mauroy Stadium or Lille Metropole Stadium.
What's nearby
- Euralille - 13 min walk
- Hospice Comtesse - 14 min walk
- Casino Barriere Lille - 15 min walk
- L'Aeronef - 17 min walk
- Charles de Gaulle native house - 4 min drive
Getting around
- Romarin Station - 3 min walk
- Lille Europe Station - 11 min walk
- Lille (LIL-Lesquin) - 10 min drive
About this property
Appart'City Confort Lille - Euralille
Along with self parking, this smoke-free residence has dry cleaning and laundry facilities. WiFi in public areas is free. Additionally, a 24-hour front desk and multilingual staff are on-site. All 134 rooms feature kitchenettes with fridges and microwaves, plus complimentary wireless Internet access and cable.
Property amenities
- Free WiFi
Parking and transport
- Self-parking: EUR 9.50 per day
Family friendly
- High chair
- Fridge
- Microwave
- Cookware/dishes/utensils
- Buffet breakfast available for a fee daily 7:00 AM–9:00 AM: EUR 11.90 for adults and EUR 3 for children
- Combined bath/shower
- Dry cleaning/laundry service
- Laundry facilities
Pet friendly
- EUR 10 per pet per day
- One-time cleaning fee: EUR 25
- Lift
- Smoke-free property
Services and conveniences
- Limited housekeeping
- Multilingual staff
- Iron/ironing board
- Blackout curtains
Location highlights
- Near a metro station
- Near a train station
- In the suburbs
Things to do
- Bike rentals and golf nearby
- 134 rooms
- 8 floors
- 1 building
Nearby things to do
- Bicycle rentals nearby
- Golfing nearby
Business-like, comfortable room, close to Lille Europe Eurostar - walking distance
Comfortable, clean, but definitely dated property, which suited our needs very well. We picked this site for its proximity to sites we wanted to visit, plus it had a kitchen and more specifically a fridge, and the walking distance to the Gare Europe. The tub was an added bonus. Pros were proximity, kitchen, tub. Cons were lack of cutting knives, toaster, actual wash cloths (they provided one sponge and one tea towel) and no vacuum or broom. They only come in and do up your room if you stay a week or more. The bedroom and bathroom were upstairs from the living room, so beware of steep stairs. Unfortunately, no one at check in informed us of how the French on demand hot water tanks work, so we ran out of hot water the first day, but that was rectified quickly. It's good for the price.
Good value for money
Very nice stay. Good value for money.
Good price and comfortable room. Hotel is closed to the center of Lille. 15 min walk or 4 minutes tram. Welcoming and helpful staff.


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Home > Cordless
A lightweight and powerful cordless brush cutter.
The STIHL FSA 130 is ideal for working on large areas of tough grass and features bike-handle control. Sold as a shell only with battery and charger available separately.
Cut even tough grass down to size
This model is supplied with a grass cutting blade and enjoys a cutting circle of 260 mm, allowing it to slice through even dense grass and cover a large area. The bike handle control of this machine facilitates a smooth mowing action when used with a harness. You can easily guide the machine over wide spaces, minimising operating fatigue.
You can choose from three power settings, which allows you to select the most suitable one for the task at hand while ensuring optimum battery efficiency.
Part of STIHL's Lithium-Ion PRO range
The FSA 130 uses STIHL's extremely energy efficient, compact and lightweight electric motor, which provides quiet operation with low vibrations and requires no servicing. In fact, this brushcutter is quiet enough that hearing protection is not required, and it weighs an impressive 4.4 kg (not including battery or charger). The latest technology also means you can operate Lithium-Ion PRO equipment in wet conditions.
This model is supplied with a single strap harness as well as the grass cutting blade.
Supplied as a shell only
Being part of STIHL's range of cordless tools means the battery and charger are both interchangeable with others in the range, allowing you to own one set and use them on multiple tools, saving money. This model is therefore supplied without a battery or charger, but if you do need to purchase one then the AP 300 (for up to 85 minutes of use) is recommended, along with your choice of charger. The AL 500 charger can recharge the AP 300 to 100% in just 35 minutes.
If you would prefer a backpack battery, a suspension accessory (part number 00000071045) should be used.
Brand: STIHLPower Source: Battery
Why choose us?
Garden Machinery Direct is a family run business with over 40 years experience in selling and servicing garden equipment. We're one of the biggest garden machinery retailers in the UK. Based in the Midlands, we're well placed to deliver products across the UK - though we do deliver internationally. We are main agents for all the biggest garden machinery brands, and have fantastic working relationships with world leading manufacturers.
Our goal is to offer customers top of the range garden machinery at discount prices, delivered direct to your door. Our friendly and professional staff are available by telephone or email to give expert advice on the equipment most suited to your needs or to answer any queries you may have. We also operate an efficient spare parts service, utilising the latest computer link-ups direct to manufacturers stocks.
All of the garden machinery we sell carries a manufacturers guarantee, supported by a nationwide network of authorised service dealers.
At Garden Machinery Direct we offer our customers the best deals and even better service.
Please feel free to contact us if you have any questions.
Be the first to Write a Review for this item!
View the STIHL brochure for more information.
STIHL FSA 130 R Cordless Brushcutter (Shell Only)£408.00 £379.00
A loop-handled cordless strimmer/brushcutter supplied with an AutoCut mowing head with a cutting circle diameter of 420 mm. This machine is sold as a shell only with battery and charger available to purchase separately.
STIHL FSA 90 Cordless Brushcutter (Kit)£521.50 - £605.00
A powerful, cordless, bike-handled brushcutter for tackling large areas of tough grass. Featuring a 250mm two-sided grass cutting blade and double shoulder harness. This product is sold as a kit, so is supplied with a battery and charger.
STIHL FSA 90 R Cordless Brushcutter (Kit)£511.00 - £594.00
A professional cordless loop-handled brushcutter, with a 380mm cutting diameter - ideal for working on large areas of tough grass. Sold as a kit, including batter and charger.
STIHL FSA 90 Cordless Brushcutter (Shell Only)£302.50
This is a cordless battery-operated brushcutter with impressive power. Including a 260mm, two sided grass cutting blade and ECOSPEED technology, this product is sold as a shell only, so requires purchasing a battery and charger separately.
STIHL FSA 90 R Cordless Brushcutter (Shell Only)£291.50
A professional battery powered brushcutter, featuring loop-handle control and an AutoCut 25-2 mowing head. Supplied as a shell only - you will need to purchase a battery and charger separately.
STIHL FSA 85 Cordless Strimmer (Shell Only)£216.00
This cordless grass trimmer has all the advantages of battery-powered machinery, while still boasting high power and excellent cutting performance. This product is sold as the shell only, so you will need to purchase a battery and charger separately.
STIHL AR 2000 Backpack Lithium-ion Battery£750.00 £630.00
These backpack Lithium-Ion batteries are ideal for highly demanding operations giving maximum capacity for longer running times. They include a comfortable ergonomic harness with integrated carry handle and rain protection cover.
STIHL AL 500 Quick Battery Charger£108.00
STIHL's quickest charger for Lithium-ion batteries, including an LED operating mode indicator and active battery cooling. Particularly ideal for use with the AR 2000 and AR 3000 backpack batteries.
HUSQVARNA S138I Cordless De-thatcher£273.00 - £458.00
Cordless machine with 2 in 1 dethatcher which will remove thatch and moss from the lawn.
HUSQVARNA 535IXP Cordless Chainsaw£290.00 - £565.26
14" Cordless Husqvarna chainsaw, designed for full-time use.
STIGA SGM 72AE Multi mate Cordless Shrub shear£69.00
7.2V Battery cordless shrub shear with double action shrub blade and grass trimming blade.


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São Paulo, Brazil – World music fans are mourning the loss of Itamar Assumpção, who lost his battle with intestinal cancer on Wednesday, June 11, 2003 at the age of 53. Mr. Assumpção struggled for more than four years with the disease and chose to leave the hospital for the comforts of home care.
Best known for mixing samba with rock, funk and reggae, Mr. Assumpção recorded seven albums in the course of his career. His last album PretoBrás was released in 1998. His music struck a chord with artists such as Monica Salmaso, Zelia Duncan Ná Ozzetti, Cássia Eller and Rita Lee, who rerecorded songs by Assumpção.Born José Itamar de Assumpção, in Tietê in 1949, Mr. Assumpção is survived by his wife and daughters, Anelis and Serena, both who share their father's passion for music and are singers themselves.


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But you will never be mature without life. This is only an issue if it's made into an issue. And at the moment, you have him captivated.
- Teaches you how to be mature.
- Success stories would be much appreciated.
- What do you think of a year-old woman dating a year-old guy?
- Five years doesn't rate as an age gap when you are an adult.
- If she doesn't know, I suggest you tell her.
21 year old guy dating 27 year old woman
He has more stamina than I do. Best of luck to everyone in this situation and if you are still around thread started it would be great to hear an update. They are living in the moment. This rule states that by dividing your own age by two and then adding seven you can find the socially acceptable minimum age of anyone you want to date. Marriage seems to be an I.
We had a lot of fun in the time we were together. Research finds that one well-known guideline may not work for everyone. He says they are just being bitches. Even women are especially older man is a.
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Relationship Talk
She needs to be dating someone more in her maturity bracket. It might be tough, but if you like her and you want it to work out, you will make it happen. We still root for each other.
Answer Questions How do I know if a guy would make a good boyfriend or just sees me as a conquest? What matters is whether your levels of maturity match, parents dating site not your calendar age. Maybe this is why the rule is so appealing.
If he's ready and understands how you feel and you openly express that, I don't see why it would be a problem. And they had data to back up something women being awesome! It didn't last, frame bicycle but he's still one of my favorite people in the world.
Are We Intuitively Honest or Dishonest? We got along great and the age difference wasn't an issue for either of us. When I got out and got my first internship, same deal. Curious outsiders are quick to judge when they can see a wide age gap between two romantic partners. As the bard said, online dating hating tumblr love the one you're with.
For example, according to find out, and like for a. Once you find yourself a job, most of your time will be spent working and accumulating money. As far as I'm concerned it's fine. We talk about a serious future together as we have very compatible life goals, but I worry him being with me is stopping him from experiencing other people and happiness in his life. The point is, that if you two are able to work through the differences that you have, it may work out for you.
You can't make somebody love you, and you can't make them stay if they don't want to. Defining love can help you figure out if you're in love. Explain to her how you're feeling.
We made a great couple, and were together for years as well. Also, I'd just like to request that you and society as a whole work super-hard to unpack yourselves of this notion. You need to mature some more. Show her you are passionate about something by developing a new hobby that most people in your age-group might not be interested in.
25 year old guy dating 33 year old woman
What s the Worst Age to Be Single - Thrillist
- Mostly because his mother doesn't approve and he still lives with her.
- The way he's not afraid to tell me how beautiful I am, to grabbing my hand and kissing it sweetly.
- If you want to date this woman, pursue that goal.
- We are all so different, though.
- If you're ashamed of her or of yourself because of her age, do her the favor of breaking things off so that she can find someone who is proud to be with her.
Having a girlfriend who is a few years older than you says nothing about you, but worrying about it does. Try as he sees her and acted like a year-old security technician. Gwyneth Paltrow is five years older than Chris Martin. Ask her out if you are ok with dating an older woman.
In other words, either a five year age difference between consenting adults is creepy or it isn't. He is a lovely guy but reality for me is that how can he take care of me when he can't even take care of himself? What says more about you is the fact that you would ask this question. Maturity might be an issue, but you'll get that in any relationship, irrespective of the age difference.
Most of the time we found out each others ages after we started dating and it just wasn't an issue for either of us. Does it match our scientific understanding of age-related preferences for dating? In that sense dating an older woman reflects well on you. It sounds like you don't respect this woman, or at least, the age difference is a deal breaker for you.
Most Popular
The minimum rule half-your-age-plus-seven seems to work for men, although the maximum rule falls short, failing to reflect empirical age-related preferences. Is that too much of an age difference? Back in the day, people married for life as teenagers. Researchers Buunk and colleagues asked men and women to identify the ages they would consider when evaluating someone for relationships of different levels of involvement. With Tao of Badass you will study on the best and his problems what the proper way to seduce girls is.
Is that a big age difference? She is going to want a guy who can support himself, just like she does. They got married two weeks ago.
This must worry you for some reason, but it shouldn't. Older women are awesome because we're well established, are independent, have careers, cool interests and do fun stuff. Let people deal, it's not a big problem unless you make it a big problem.
This has likely changed somewhat given that i married yet. Is that really who you want to believe? It might be tough for you to be the man she wants you to be, is all I'm really trying to say. This man adores me and I haven't had that in a very long time.


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A Christmas Carol November 26, 2009Posted by Shujath in English, Movies, Reviews.
Tags: 2009, A Christmas Carol, Alan Silvestri, Bob Cratchit, Bob Hoskins, Box Office, Disney, DVD, Ebenezer Scrooge, Film, Gary Oldman, Ghosts of Christmas, Hollywood, ImageMovers Digital, IMAX 3D, Jim Carrey, London, Motion Capture, Movie, Performance Capture, Review, Robert Zemeckis, Robin Wright Penn, Tiny Tim, VFX, Walt Disney Pictures
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I wonder if Charles Dickens' beloved classic was really suited for a 3-D motion capture adaptation. Except the promising first few minutes, most of what comes after turns out be a rather bland and soul-less rendering of Dickens' tale.
In recent times, Robert Zemeckis seems to have lost his touch even though we can overlook The Polar Express and Beowulf purely for their technological innovation of a new form of storytelling. Sadly, A Christmal Carol is a pretty late arrival to even get away with the "technology" excuse. Ebenezer Scrooge's original tale was both bleak and touching (still one of the very few stories I really loved as a kid) and where the film falters mostly is in the latter aspect. Jim Carrey does his best in multiple roles and that's definitely among the very few things which make this movie watchable.
You might want to watch this if this is your first IMAX-3D flick – or better still wait till December 18th to catch the real biggie.
The Dark Knight July 19, 2008Posted by Sai in English, Movies, Reviews.
Tags: Aaron Eckhart, Alfred, anarchy, Batman, Batmobile, Batpod, blockbuster, Bruce Wayne, Christian Bale, comic book, Commissioner Gordon, crime, DC Comics, English, Gary Oldman, Harvey Dent, Heath Ledger, hero, Hollywood, Joker, Lucius Fox, Maggie Gyllenhaal, Michael Caine, Morgan Freeman, Rachel Dawes, sequel, superhero, The Dark Knight, The White Knight, thriller, Two Face, vigilante, villain, Welcome to a world without rules, What doesn't kill you makes you stranger, Why so serious?, You complete me
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The most awaited movie this summer finally arrives in theatres. Film geeks have been churning out post after post on the blogosphere awaiting its arrival and the phenomenon isn't going to stop post-release. Almost every critic worth his salt has raved about it. If you thought Batman Begins was super, wait till you watch The Dark Knight. It meets all the expectations and then some.
A question that everyone is asking themselves is whether this is the best superhero movie ever. Before you go there, you might want to ask yourself if it is a superhero movie. Batman was always one of the most identifiable superheroes because he didn't have real superpowers. In his two Batman films, director Christopher Nolan (who has dabbled in noir more often than not) has employed a dark tone and a lot of logic to make Batman feel very real. He continues that in this film, making it feel like a crime thriller more than a superhero movie. If we still were consider it a comic book superhero film, I'd say it tops my list (and that of so many more).
The film is centred around three major characters. Harvey Dent, the white knight of Gotham, who provides people with the hope that he can change things for the better. Batman, the dark knight of Gotham, whose work seems to have worsened the crime in the city. And finally the Joker, a psychopathic killer who terrorizes the city with his own crazy, unpredictable but believable motives for doing so. Will the white knight take Gotham forward? Will the Joker ruin Batman and Gotham? Can the Batman still stay incorruptible?
The screenwriters (Jonathan and Christopher Nolan, who've worked together on Memento and The Prestige) devise this film to take forward the story of Batman and Gotham. The film isn't about superheroes. It is about criminals and crime fighters and how they affect each other. It is about the emotions, the motives, the psyche. It is about rules. It is about those who live by them and about those who follow none. The remarkable screenplay is driven around these ideas and not around the villains or their dumb ideas for world domination or the action sequences. Newer situations and conflicts are created, ensuring that the movie doesn't feel repetitive (and that is always a problem for sequels). Everyone has a good reason for their actions. Everything is as realistic and logical as it has ever gotten in a comic book film. Christopher Nolan (Batman Begins, Following, Insomnia) created a successful reboot for a dead franchise and he takes it a step further this time. His contribution to the film – the detail, the tone, the vision, the execution – is superlative.
I loved the designs of the vehicles, weapons and the sets the first time (production design by Nathan Crowley). They are even better here. Wait till you see the Batpod in action. I was totally blown away by its introduction in the film. The action sequences are also much better this time around. The Joker's makeup is very natural and the extended lips create a great effect. But the best part is the visualization of Two Face. It could scare the shit out of many.
Christian Bale continues his wonderful work (I especially like what he does with his voice for Batman) in the role that opened many doors for him. The late Heath Ledger brings the Joker to life in a delightful performance. Aaron Eckhart is well cast as Harvey Dent and he very much feels like someone whom people can instantly like and put faith in. Maggie Gyllenhaal is a suitable (many will say better) replacement (for Katie Holmes) for the part of Rachel Dawes, who is caught between the two knights (no, it isn't a perfunctory love triangle). Michael Caine as Alfred Pennyworth and Morgan Freeman as Lucius Fox play their supporting parts to perfection while Gary Oldman as James Gordon is just as convincing and even more integral.
Now, after all the praise the question still remains. Should you watch it? The answer isn't a resounding yes. If you've liked Batman Begins and have caught a whiff of the hype, you're probably going to see this (if you haven't already) irrespective of my opinion. But there are others who didn't like that film much. Some found it too dark. Some found that the action or entertainment wasn't enough. Others found it complex. Maybe they expected a popcorn movie and ended up with something else. If you are one of those, I wouldn't particularly push you to watch this.
P.S. As I eagerly await Nolan's sequel to this film, I prepare myself to understand that it will be hard to top this. So, anything that is at least close to matching the original is good enough for me.


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No matter what age you are, you are at risk for bruxism. It is more likely in children and young adults then it is in adults, but can happen in almost anyone. The good thing is there is a variety of options for bruxism treatment for people of all ages. There are a number of things that can trigger you to grind your teeth no matter young or how old you are. Stress is one of the most common triggers when it comes to teeth grinding, but there are a number of other factors to consider as well.
Anxiety and Stress
Teeth grinding may be related to emotional or mental difficulties, like tension and worry. Studies have demonstrated around 70% of instances of sleep bruxism are due to tension or tension, which changes individuals subconsciously while they are asleep. Elevated rates of work-related anxiety may get a significant adverse effect in your sleep-related grinding episodes.
Being concerned or distressed may cause one to clench your jaw or grind your teeth. Bruxism may also occasionally be due to taking certain antidepressants used to treat stress and depression.
Sleep Disorders
Studies have revealed people who snore or have a sleep disorder, like obstructive sleep apnea, are more prone to grind their teeth while they are asleep. OSA causes your breathing while you sleep, to be interrupted.
The connection between OSA and bruxism has only been identified pretty recently, and also the precise connection between the two states is not entirely understood.
- You're also more likely to grind your teeth
- You talk or mumble while sleeping
- Act out while asleep, like kicking out or striking
- You have sleep paralysis (a temporary inability to move or talk while waking up or falling asleep)
- You experience hallucinations (seeing or hearing things that aren't actual) while you are semi-conscious.
Bruxism can sometimes happen as a side effect of taking certain kinds of drugs. These generally include some psychotropic medications which affect your mood, like antipsychotics and antidepressants.
In particular, bruxism may also be linked to a form of antidepressant known as a selective serotonin reuptake inhibitor. Examples of SSRIs include paroxetine, fluoxetine and sertraline.
You might not develop any symptoms, although there is a higher risk of developing bruxism in the event you are taking these drugs. Talk to your doctor before you start taking any type of medication because it can worsen your bruxism.
There are a number of factors that you need to consider as possible reasons for your bruxism. You will not know for sure until you see your dentist and he can provide you with an official diagnosis. There are a number of treatment options available as well.


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Not that many years ago (ok, a bit longer than that), my husband and I had that same question. We were ready to put our monthly rent funds into something more permanent, lining our own pockets instead of the landlord's. Though I'd entertained becoming an agent for many years, I didn't have a notion of how to CHOOSE one to help us on our search.
We were looking for small acreage properties, and since I was already tech savvy (yes the internet worked back then!), I went wild looking up properties in our price range and area. I did reach out to some agents via those slicky property websites. More often than not, I didn't get an answer, or at least one within the same 24 hour period in which I quested. Being tech savvy, I wanted my answers NOW, so those agents didn't get my business.
Using the shiny new internet, I then searched for websites of brokerages near the areas I was searching. I'm a face person; meaning, if you have a nice, honest seeming face, I wanted to know more about you. I also was fairly anti-corporate; having worked in IT for many at this point in time made me a bit wary of the 'branding' and tactics.
I found a website for a seemingly low key, independent brokerage. There was a picture of a family of 5, all smiling and happy, with cute little kidlets. Clicking the "About Us" choice, I saw a list of agents that worked in that office. I simply chose the first face that looked like a person I'd like to work with, and called to speak to her. "Cathy" was very pleasant, and as I explained to her what I was looking for, and that I was looking at these internet sites and would likely tell her what I wanted to see, she happily agreed.
Being a horseperson, when I look at properties I generally walk the fencelines and go to the barn first. When we all met for the first time at the first property, it was October. Lots of rain, and long grass. Cathy showed up in a lovely outfit and high heels. I mentioned kindly that we'd go out to the fields first, and she could wait for us. She perkily said she'd come on out with us. Wet, grass seed laden feet later, she was still perky, but I gather she'd learned a lesson. Boots of some sort or another were the next meetings' choice of footwear for Cathy.
We did eventually put an offer on a place, and I recall she was helpful and the process didn't seem too crazy, even though I called her a bunch to check up on how things were. All was well in the end.
We DID, however, hire a horse properties expert to SELL that property. I knew enough by then to see how much of a difference it could make. She was awesome; dealt smoothly with all my detailed requests and a horseperson bought the property. I used the 'face' tactic again, when a friend-referred agent turned out to be less than reliable. My 'face' agent, however, WAS a horseperson as well, this time.
The point to that story is that we didn't really focus on someone 'horsey' to help us at first. That decision didn't cause us a problem that I know of, since I didn't at that time know all the things that acreage properties can offer as issues. I'm not sure she'd have understood them, either. My 'face' tactic worked that time. Later I found out the broker was not all smiles as his family picture made him seem, but it didn't affect our transaction.
I still use the 'face' tactic in other places, but I now know to meet that person first, see how I feel in their 'aura', their airspace, while talking with them. I don't need a rocket scientist usually, but I do want someone who is genuine, not too flashy, and knows something about the subject matter at hand. I can tell fairly fast if I will not like working with someone, whether impressive credentials come with or not.
A real estate transaction can last from 3-12 months, depending on your property and the market, even if you're a Buyer. If you have 'the willies' around your agent, chances are your needs won't be met because you may be too worried about rocking the boat. Real estate agents are people, too, and the good ones are in YOUR corner at all times. We should be giving you sound advice based on market and our previous knowledge, though, not just a 'yes man'. I wish our first agent had pointing out the drainage issues with that house that my starry-eyed love of it's barn overlooked.
Make sure you interview (meaning, go have a cup of coffee with) a couple agents before deciding who's going on this roller coaster ride with you. Friends are great, but keep in mind that sometimes the ups and downs of the transaction may ding that relationship. And even though your friend used an agent they were happy with, you may feel differently about that agent's style. A friendly local agent is great, but you have to decide if they have the business acumen to get the job done. Check them out over coffee, too. Be upfront, and say you're checking out a couple agents before you decide. This can give you yet another indicator of how that relationship can go. It's a bit like dating; you don't necessarily leap at the first offer, right? Only you know if it's right. It's your investment; make it your clear decision on who will help you buy or sell that investment.


text: Lisa Kay Photos Overview Filmography Photos Lisa Kay at the premiere of "Evelyn." Lisa Kay at the premiere of "Evelyn." Replay Slideshow Previous slide Next slide Thumbnails Play Share Share on Facebook Share on Twitter Share on Google+ Share on Tumblr Share on Pinterest Share by Email Maximize 1 of 2 Advertisement


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All Payroll Attendance Call Answer Service
How can I see the timesheets and paystubs of a deleted person?
Answer. Find the deleted person in Home > Deleted, then go to their calendar.
Just because a person has been deleted, you're not stopped from seeing their timesheets and paystubs. To see them, first you'll need to navigate to the person:
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Editor's Note: What does the Russian Revolution have to do with World War II? Everything! First of all, it was not a revolution but rather a coup de' etat planned and executed by Jewish bankers and terrorists. Everyone in Europe was horrified by what was occurring in Russia, and feared it would spread. Attempts were … Continue reading Jewish-Bolshevik Destruction of Christianity in Russia
Category: Bolsheviks
Behind An Eye for An Eye: Revenge, Hate and History
Original source: http://www.ihr.org/jhr/v20/v20n1p-9_Sack.html Three years ago I was scheduled to speak at the United States Holocaust Memorial Museum. The speech was announced in this brochure and also on the Internet. But then the Museum canceled it. For the next forty-five minutes, I'll say here what I'd planned to say at the Holocaust Museum, and then, just … Continue reading Behind An Eye for An Eye: Revenge, Hate and History
Communism is Judaism, Period
Original Source: http://www.truthfromgod.com/articles/jews_created_communism.html Today there is a lot of talk about fighting Communism. The only problem is that the American people are not aware of who are the true founders and promoters of Communism. If you had to find a Communist, where would you look? Would you recognize a Communist if you saw one? What … Continue reading Communism is Judaism, Period
Christopher Jon Bejerknes Peddles Tired Old Jewish Lies About Hitler and National Socialism
Recently, Christopher Jon Bejerknes was recently on the "No More News" show with Adam Green on Youtube. Adam Green, never really heard of him until now, gave Bejerknes over 2 hours to regurgitate old Jewish and "court historian" lies about Adolf Hitler and National Socialism. He recently has come out with a new book, "of … Continue reading Christopher Jon Bejerknes Peddles Tired Old Jewish Lies About Hitler and National Socialism
The Secret Masonic Victory of World War II By Dennis Wise
Original source: https://thesecretmasonicvictoryofww2.tv/ And at Bitchute: https://www.bitchute.com/channel/truthwillout/ Western Freemasonry and their Masonic funded Eastern Communist friends won World War 2. Everything we see today stems from that Jewish Masonic victory. All Freemasons in politics know the aim is a one world communist utopia. All policies today aid the minorities aimed at breaking down the family, … Continue reading The Secret Masonic Victory of World War II By Dennis Wise
EUROPA – The Last Battle ~ The Full Documentary (2017)
Source link: https://europathelastbattle.wordpress.com/watch/ HAVEN'T YOU EVER AT LEAST BEEN CURIOUS AS TO WHAT "THE OTHER SIDE OF THE WORLD WAR II STORY" WAS? Since the mid-20th century, the world has only ever heard one side of the most horrific war in human history. During the 75 years that have now passed, only a single narrative of … Continue reading EUROPA – The Last Battle ~ The Full Documentary (2017)
In Stalin's Gulags
Editor's Note: The alleged Holocaust is the most over publicized non-historical event in history! Yet, real holocausts like the millions who perished in the Soviet Gulag System (see Gulag Archipelago by Aleksandr Solzhenitsyn) are never mentioned. Once you investigate the massive amount of death that was inflicted by the JEWISH run gulag system in the … Continue reading In Stalin's Gulags


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As a mother, woman of color, person of faith and union member, the horrific tragedy that occurred in Charleston last week has been incredibly painful to comprehend. Nine people were killed in an "unfathomable" act of violence at a historic predominantly black Charleston, South Carolina, church Wednesday evening when a 21-year-old gunman opened fire during a prayer service.
Sadly, Labor did not escape this senseless act. A union brother Tyrone Saunders (ILA member in Charleston) lost his son and aunt who were attending bible study at Emanuel AME Church that night. Without a doubt this must have been the most difficult Father's Day of our brother Tyrone's life. From civil rights to human rights, Faith and Labor have walked hand in hand in the fight for the issues that directly affect all of our countries working families. As Union sisters and brothers across the state of Florida, now is the time for us to step up and support those directly affected. Together we can act by praying for the victims' families, accepting and fighting against the hate and racism that fueled this horrendous act, but also join Faith in finding it within ourselves to extend the love and forgiveness for the young man that decided to take these nine lives.
In the wake of this horrific tragedy, I am still filled with hope seeing our unity as a movement. The outpouring of support from working families who reached out beyond our "Union bubble" to support our partners in the Faith Community when they needed us most, as we spoke out and prayed together in vigils across the state this week. As Emanuel AME Church and the City of Charleston heals and moves forward, working families can show their support by leaving your porch lights on this evening in remembrance of the victims of this tragedy and as a symbol that now more than ever, WE ARE ONE.
Salandra Benton is a Florida AFL-CIO Community Organizer.


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Hinko - the ICT flag is nothing new. It does/will apply to HD broadcasts worldwide. I believe Sky currently use this on their content now to prevent output of a HD signal over component on certain HD channels.
The HDCP standard is designed to enforce this protection - nothing more, nothing less. It provides no other good the consumer, and in many cases turns otherwise reasonable equipment into lemons as you have found out.
The ability to protect the path (via HDMI or via a protected Digital source) has been in Bluray from day one. I believe this was an initiative of Sony, along with regional encoding. Many titles have chosen not to use it so far, but it will get turned on. HDCP was also used in HD-DVD.
Much of this stuff has been documented for years now. There is nothing new, hidden or otherwise. The media companies have been very public in their desire to protect their content. They have spent billions of dollars with DRM companies to enforce their rights.
The consumer in this case is comes a distant second to the desire of the media content companies to protect their content using DRM Tools, ICT flags, DCP on Bluray etc etc etc. There is nothing new here, and has been happening for years.
If you desire to use certified devices to view HD content, such as Freeview certified decoders and PVR, and or MySky and MySky HDi, these protection mechanisms will exist, and you will need to learn to live with them. Remembering you choose to purchase their equipment. When you watch a DVD or Bluray, you agree to abiding by the usage rights that go with it.
Broadcasters will have the ability to time limit things such as recordings to say 48 hours or 1 month, or whatever they choose. If you use these devices get used to it. You will also need to use equipment which supports these standards ie HDMI at a minimum.
On the flip side, the billions of dollars spent on content protection (usually with trumpeted media releases saying how many billions of dollards they have spent on them, and how good they are) to limit the functionality of these devices is balanced by the zero dollards spent by snotty nosed geeks who never see the light of day, eating 5000 pizzas a year, but destroy these protection mechanisms days after they are released by the content providers. They then proceed to write software for normal people who can then use these to ignore all of the above, and continue to watch TV, Movies and record stuff like they always have.


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