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Office of Safe and Drug-Free Schools
Completing Forms for a Discretionary Grant
Here are some tips to help you complete the Federal forms required to submit an application for funding from the Office of Safe and Drug-Free Schools (OSDFS). This information should be used as a supplement to the OMB-approved directions that accompany the official forms.
You will need to submit the following forms with your application:
- ED Form 424--Application for Federal Education Assistance
- ED Form 524--Budget Information, Non-Construction Programs
- Standard Form 424B--Assurances, Non-Construction Programs
- ED80-0013--Certifications Regarding Lobbying; Debarment, Suspension, and Other
- Responsibility Matters; and Drug-Free Workplace Requirements
- Standard Form LLL--Disclosure of Lobbying Activities
ED80-0014--Certification Regarding Debarment, Suspension, Ineligibility, and Voluntary Exclusion -- Lower Tier Covered Transactions
Your agency's authorized representative (usually not the project director) must sign each form. Electronic versions of these Federal forms and the OMB instructions can be found at www.ed.gov/offices/OCFO/grants/appforms.html.
ED Form 424-Application for Federal Education Assistance
This is the first page of your application and contains all of the administrative information ED needs to make a grant award. The form contains 15 blocks: 1-8 requests information about the applicant; 9-13 asks information about the application; 14 asks for the estimated (yearly) funding, and 15 asks information about the person who is authorized to legally bind the applicant entity. Below is a brief description of the information we are looking for in each block.
Applicant Information
Name and Address is the name of the organization applying for the grant, for example, Enterprise School District, and its mailing address. The applicant will have fiscal and administrative control of the grant award funds. The Organizational Unit refers to the division within the applicant's organization that will be responsible for the grant (e.g., Office of Curriculum and Instruction). If you are unclear about the Organizational Unit for the applicant, leave the box empty.
The D-U-N-S Number is a nine-digit number provided by Dun & Bradstreet, which provides unique identifiers of single business entities. This number does not convey any information about the recipient - a built-in check digit helps assure the accuracy of the number. If you do not have a D-U-N-S Number, you may obtain one at no charge by calling 800/333-0505 or by completing a D-U-N-S Number Request Form on the Internet at www.dnb.com.
TIN (Tax Identification Number) should be available through your organization's accounting office.
Catalog of Federal Domestic Assistance (CFDA) number designates the competition under which you are applying. Every Federal grant program has its own identification number. You can find the CFDA number for the competition in the Federal Register notice and the application package. Title is the title of the grant competition. An example of a CFDA number and title is 84.215F, Carol M. White Physical Education Program.
The Project Director is the person who will oversee the day-to-day operation of the grant program. If that person has not been selected, provide the name and complete contact information of someone who can answer questions about the application if we need additional information.
Novice Applicant is defined in the application package if the grant program will give special consideration to a new applicant. You should read the notice in the Federal Register and the application package carefully to determine if this consideration will be applied to the grant competition under which you are applying.
Debt Delinquency. If the organization applying for the grant is delinquent in any debt to the Federal government, such as audit disallowances, loans, or taxes, you should check "Yes" and include an explanation of the issue.
Type of Applicant refers to the organization applying for the grant. The Federal Register notice and the application will include information about the types of organizations that are eligible applicants for a specific competition. Check the one that most closely describes your organization.
Application Information
Type of Submission is Application, Non-Construction for discretionary grants from OSDFS.
Executive Order 12372 applies to all OSDFS grants unless the applicant's state chooses not to participate. Check the State Single Point of Contacts list found in the application package to see if yours is a participating state. If your state does participate, you must submit a copy of your application to the state contact listed in the application package and list the date the letter was sent in this section of the application. Failure to do so may result in loss of a grant award. If your state declines to review your application, check the second box under "No - Program has not been selected by State for Review." If your state is not included in the Single State Point of Contact list, you are not subject to Executive Order 12372 and should check "No - Program is not covered by E.O. 12372."
Proposed Project Dates should reflect the entire time period of the grant. Grant length varies according to the program's statute; the Federal Register notice and the application package will have guidelines for the length of the grant. Unless otherwise directed, you should keep these dates in line with the Federal fiscal year: October 1 through September 30.
Human Subjects Research applies to all applicants that plan to conduct research activities involving human subjects at any time during the proposed project period. Research is defined as an activity following a deliberate plan whose purpose is to develop or contribute to generalizable knowledge, such as an exploratory study or the collection of data to test a hypothesis. For additional information about the protection of human subjects in research, check the Department's Protection of Human Subjects in Research Web site at http://www.ed.gov/about/offices/list/ocfo/humansub.html.
Title of your proposed project should be included in this section.
Estimated Funding
Estimated Funding should reflect the funds requested for the first year of the project only. Federal refers to the amount of funds requested from ED. The other categories are sources of additional funding, if any, to support the program. Some competitions require matching funds from the applicant. Check the Federal Register announcement and application package to find out about the requirements of a specific program.
Authorized Representative
The Authorized Representative is the designated representative of the applying agency. This person is authorized to commit the applying organization to the financial and programmatic plans set forth in the grant.
ED 524 Form-Budget Information, Non-Construction Programs
This form is divided into three parts, each of which must be provided, as applicable.
Section A is required of all applicants. In this section, you must list by budget category all funds you are requesting to operate your program.
Section B may be optional, depending on the requirements of the program. Here you will record the non-Federal funds to be used to support the program, such as matching or in-kind funding. Not every competition requires matching funds. Check the application package and Federal Register notice for the program in which you are interested to see if it has a matching requirement. Remember that ED staff will monitor matching funds along with expenditures of Federal program funds. If you offer a match, you will be held accountable to provide it even if the program does not require a match.
Section C is required of all applicants and is the budget narrative portion of your application. In this section you must provide a detailed explanation of all proposed expenditures for your project. These expenditures should be carefully linked to the goals, objectives, and activities described in the project narrative portion of your application. ED staff will review all information about your proposed expenditures and determine whether the expenditures are allowable (either permitted or not specifically prohibited), allocable (can be directly allocated to your project); and reasonable (costs that would be incurred by a "prudent person"). Any costs not fully described or justified can be deleted from your funding request during the budget review conducted by our staff. As you prepare your budget narrative, keep the following in mind:
- Only project staff members that are employees of the applicant should be in this category.
- Staff who are working on the project and are being paid with an in-kind contribution or whose salaries are included as part of a cost-sharing/matching requirement should be included in this category under Section B.
Examples include but are not limited to key personnel (e.g., Project Director, Project Coordinator), professional staff (e.g., trainer), paraprofessionals, and support staff.
Fringe Benefits
- This category includes the direct cost of all fringe benefits paid to staff whose salaries will be paid by the grant.
Examples include but are not limited to life insurance, health insurance, retirement plan, and vacation and sick pay.
- All travel must be justified and clearly linked to the goals and objectives of your project.
- Travel costs are expenses for transportation, lodging, subsistence, and related items incurred by employees who are in travel status on official business of the organization.
- Identify all staff that intends to travel, and include destination and duration, number of trips, and cost per trip.
- Document the established mileage rate for any local travel.
Check the application package for information on ED-required travel for project directors and others.
Equipment is tangible, non-expendable items having a useful life of more than one year and an acquisition cost of $5,000 or more per unit. However, consistent with the grantee's policy, the grantee may establish lower limits.
- Supplies or materials are tangible, expendable items. Direct supplies and materials differ from equipment in that they are consumable, expendable, and have a relatively low unit cost. Computers are supplies if their unit cost is less than $5,000.
Supplies and materials purchased with grant funds should directly benefit the grant project and be necessary for achieving the goals of the project.
- Since discretionary grants available through the Office of Safe and Drug-Free Schools are Non-Construction grants, no costs should be listed in this category.
Minor remodeling is acceptable, such as putting a fresh coat of paint on office walls, as long as it is allocable to your project. These costs should be placed in the "Other" category.
- For purposes of a discretionary grant, a contract is a written binding agreement between two or more parties for the purpose of acquiring property and/or services in accordance with specific terms and conditions. Property and/or services procured under a contract arrangement should directly benefit the grant project and be necessary to achieving the goals of the project.
- All products or services delivered by a contractor should be fully described and justified.
Examples include but are not limited to professional services (e.g., consultants, evaluators, trainers), leases (e.g., office equipment, office space), miscellaneous services (e.g., transportation, printing) and maintenance/repair (e.g., building maintenance).
- This category is where applicants may place all direct costs that are not clearly covered by the other direct cost categories. This category may include a wide variety of costs that do not seem to fit elsewhere in the budget.
- Be sure to avoid the term "miscellaneous," and fully describe and justify all items.
Examples include but are not limited to communication costs (e.g., telephone, postage) and conference registration fees. Release time for teachers to attend training or substitute teachers compensation should be put in this category, not in personnel.
Total Direct Costs are the sum of lines 1-8.
Indirect Costs
- Indirect costs are defined as charges an organization incurs that benefits all projects generally and not one in particular, and they cannot be assigned to specific activities of a project.
- Examples may include but not be limited to utilities charges and facilities charges (e.g., telephone usage, rental fees).
- Be sure to check the application package to determine if any restrictions have been placed on indirect costs.
- If no restrictions have been placed on indirect costs, you may charge the negotiated indirect cost rate that has been established between the applicant and cognizant Federal agency. For local educational agencies, the allowable indirect cost rate is established by the State Department of Education.
For more information about indirect cost rates, please contact the Department of Education's Indirect Cost Group at 202/708-7770 or visit www.ed.gov/offices/OCFO/intro.html.
Training Stipends
There are basically three types of projects that might include budget requests for stipends, tuition allowances, or other types of similar charges to support the objectives of the project: fellowship or scholarship programs, educational training projects; and projects where students receive tuition remission or other forms of compensation as, or in lieu of, wages. If teachers are paid overtime to receive training after working hours or on weekends, the costs are added to the Other category.
Total Costs (lines 9-11)
ED80-0013--Certifications Regarding Lobbying; Debarment, Suspension, and Other Responsibility Matters; and Drug-Free Workplace Requirements
The purpose of this form is to certify that the applying organization has not and will not lobby Congress in connection with the grant, and that if any non-Federal funds have been used to lobby, this information will be disclosed on Standard Form LLL; the applying organization is not debarred, suspended, or proposed for debarment, has not been convicted of fraud, and is not currently being indicted; and the applying organization, if awarded, will maintain a drug-free workplace.
Certification Regarding Debarment, Suspension, Ineligibility and Voluntary Exclusion-Lower Tier Covered Transactions
If the applicant proposes to use contracts to carry out a portion of the work, the contractor (not the applicant) must sign the certification. If contracts will not be used, mark "not applicable" on the form.
Standard Form 424B--Assurances, Non-Construction Programs
The purpose of this form is to certify that the applying organization and its authorized representative are aware of the legal requirements of the grant and are willing to comply with them.
Standard Form LLL--Disclosure of Lobbying Activities
The purpose of this form is to disclose any lobbying activities that use any non-Federal funds to influence Congress in the grant-making process. If this does not apply to your grant, you should mark the form as "Not Applicable" and include it in the application.
General Education Provisions Act (GEPA) Requirement - Section 427
This requires an applicant, as a condition of receiving a grant, to identify barriers to equitable participation in its federally assisted program and to provide a description of the steps proposed to overcome those barriers. Barriers might include gender, race, national origin, disability, age, language background, or other unique factors in the setting in which the program will be carried out. The application package contains a full explanation of how to meet this requirement. Note that a general statement of the applicant's nondiscrimination policy is not sufficient to meet this requirement.


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Edwin G. Krebs, a giant of biochemistry in the 20th century, died Dec. 21 in Seattle. He was 91. His discovery of protein phosphorylation as a regulatory mechanism (with Edmond Fischer) touched all aspects of biomedical science and profoundly influenced therapeutic approaches now widely used in clinical care. Ed's life story epitomizes his commitment to family and colleagues, excellence in research and service to the biochemical community.
Ed Krebs was born in Lansing, Iowa, on June 6, 1918, the son of a Presbyterian minister and a schoolteacher. His father died suddenly when Ed was 15 and, at the height of the Great Depression, the family moved to Urbana, Ill., for financial reasons. It was there that Krebs completed high school and earned a degree in chemistry from the University of Illinois in 1940. As an undergraduate, he became enamored with organic chemistry but eventually chose to become a physician, largely because he won a scholarship to attend Washington University School of Medicine in St. Louis.
The principal responsibility of a medical school during the war years was to train physicians for the armed forces. However, Krebs also was encouraged to participate in "medical research." After graduating from medical school in 1943 and doing 18 months of residency training in internal medicine at Barnes Hospital in St. Louis, Krebs went on active duty as a medical officer in the Navy. After being discharged from the Navy in 1946, Krebs returned to St. Louis with plans of becoming an academic internist. However, all of the hospital positions were filled by returning veterans, and, as a temporary measure, Krebs was advised to study basic science as a postdoctoral fellow at Washington University. Largely because of his background in chemistry, he was accepted into the laboratory of future Nobel laureates Carl and Gerty Cori in the department of biochemistry. After two years in the Cori lab doing research on the interaction of protamine with rabbit muscle phosphorylase, Ed became so captivated with biochemistry that this intially reluctant biochemist never returned to internal medicine. The next step was to find a permanent faculty position.
During his naval service, Ed's ship had gone to Seattle. The tranquil waters of the Puget Sound and the natural beauty of the city left a lasting impression. So, in 1948, he happily accepted a position as assistant professor of biochemistry in the fledgling University of Washington School of Medicine. Under the capable leadership of Hans Neurath, the department of biochemistry was being expanded to incorporate expertise in protein chemistry and enzymology. This included the recruitment of Edmond Fischer in 1953— a talented and charismatic Swiss biochemist with experience in the enzymatic analysis of potato phosphorylase. Thus, a lifelong friendship and a formidable research partnership was forged.
Together, Ed (Krebs) and Eddy (Fischer) determined the mechanism by which 5'-AMP served as an activator of phosphorylase b. They found that ATP was required for phosphorylase activation and, in a somewhat unusual experiment, discovered that calcium, leaching from filter paper used to clarify the extract, was an important co-factor. By using gamma-32P-labeled ATP, they demonstrated that phosphate was incorporated into a specific serine residue of phosphorylase, thereby yielding the activated phosphorylase form. This landmark paper was published in the Journal of Biological Chemistry in 1955 (1). Subsequently, Krebs, Fischer and colleagues confirmed that this phosphorylation is mediated by a phosphorylase kinase, which is itself controlled by a cAMP-responsive kinase, leading to the idea of a kinase cascade. In 1968, Krebs purified this cAMP-dependent protein kinase (PKA).
At this point in his career, interests in teaching and certain aspects of administration motivated Krebs. In 1968, he was attracted by the opportunity to become the founding chairman of the department of biological chemistry at the University of California, Davis, and stayed for a period of eight years. Ed also embarked on his long association with the American Society for Biochemistry and Molecular Biology, initially by joining the editorial board of the Journal of Biological Chemistry. In 1972, he became associate editor for the journal and remained in this position for more than 20 years. He also served as the president of ASBMB in 1985. In 1977, he returned to the University of Washington as chairman of the department of pharmacology. What he liked most about both positions, he said, was the responsibility of selecting good faculty members for the departments. At UW he was also appointed an investigator for the Howard Hughes Medical Institute.
After achieving his goals as department chair in 1983, Krebs refocused his efforts on research and training junior scientists. At this later stage in his career, he set his sights on solving new problems in signal transduction. His laboratory contributed to the analysis of phosphotyrosine signaling events and published key findings that were instrumental in the discovery of a new phosphorylation cascade— the MAP kinase pathway.
Krebs received many major scientific awards for his insights into the principles governing cellular regulation in health and disease. Among those honors were election to the National Academy of Sciences (1973), the Passano Foundation Award (1988), the Horwitz Prize (1989), the Lasker Research Award (1989), the 3M Life Sciences Award (1989) and the Welch Award in Chemistry (1991). At age 74, he and Edmond Fischer were honored with the 1992 Nobel Prize in Physiology or Medicine for the discovery they made almost 40 years before and its ongoing influence in many scientific and biomedical fields.
In 1997, Ed finally closed his lab but remained a fully committed ambassador for biomedical research at the University of Washington. He was frequently spotted wandering the halls of the medical center on his way to hear the latest and greatest results in a research seminar. Ed is survived by his wife of 64 years, Virgina (Deedy) Krebs, children Sally, Robert and Martha and several grandchildren.
Edwin G. Krebs will certainly be remembered for his keen intellect, astonishing research productivity and iconic status within the biomedical research community. He was a beloved mentor to numerous students and postdoctoral fellows. Those who were privileged to work closely with him will remember him fondly as a kind and gentle mentor who passed on extraordinary insights in a quiet and dignified manner. The legacy of this self-proclaimed "reluctant biochemist" should be a wonderful inspiration to the next generation of our profession.
Below, we offer reflections from several of Krebs' friends and colleagues.
I was very saddened by the passing of Ed Krebs. Even though I had not seen Ed for several years, I still considered him a close friend.
We are all familiar with his monumental work on protein phosphorylation. Less known is his importance to biochemistry as a member of the editorial board and as an associate editor of the Journal of Biological Chemistry. His influence on the development and operations of the JBC was particularly important during this period of rapid expansion for the journal. We always depended on his advice and thoughtful consideration both on the overall operation of the journal and on the review of specific manuscripts. Authors often commented on the thoroughness and fairness of his reviews.
We had many wonderful interactions over the years, due in large part to his commitment to the JBC. Ed was a superb scientist and a nice person, and I— and all of the associate editors— feel lucky to have known and worked with him.
Herbert Tabor
Journal of Biological Chemistry
I enjoyed working with Ed Krebs for five years as an associate editor of JBC. I had known him when he was a faculty member of the department of biochemistry at the University of Washington School of Medicine in Seattle, but I saw him in a new role with JBC. He always had good advice in matters we considered at our associate editor meetings.
Robert L. Hill
James B. Duke professor of biochemistry
Duke University
Ed Krebs and Ed Fischer were two icons in the field of reversible phosphorylation. When my laboratory group started its work on protein phosphatases, they were both very welcoming to me and to members of my group. Their personalities made the field better, and they set the standard for exemplary scientific behavior. I also had the privilege of giving the Ed Krebs Lecture a few years ago, an event I still remember very fondly and treasure to this day.
Jack Dixon
Professor of pharmacology, cellular and molecular medicine and professor of chemistry and biochemistry
University of California, San Diego
I was so sad to hear of the passing of Ed Krebs. He was one of the true gentlemen in science. I owe any success that I have had to his willingness to take on a young and very naïve scientist from Wyoming as a postdoctoral fellow. His lab was a place where you could propose and pursue your own projects under his subtle, but always insightful, guidance. I remember once returning from an interview for a faculty position that I was not offered, probably due in part to my inherent laconic personality. Ed gently pulled me aside, put his arm around my shoulders and said, "Bob, the next time you go on a job interview, take some amphetamines."
Robert Geahlen
Professor of medicinal chemistry and molecular pharmacology
Purdue University
My fondest memories of Ed involved our annual laboratory research retreats. These were held over a couple of days and nights in a place called Pack Forest, a conference center that was owned by the University of Washington's School of Forest Resources. It was not the Hilton. It was a former logging camp, where you slept in bunkhouses, showered in a communal bathhouse, gave your research presentations in a big log cabin heated by a stone fireplace and ate hearty food in a rustic cafeteria. Away from the office, the phone, etc., Ed doffed his coat and tie and donned his jeans and flannel shirt. He joined us for hikes, softball (he broke his hand one year) and Frisbee. There, he'd let his guard down a little and share stories about life. The one that sticks in my mind was the awkward moment when he arrived in Seattle to start work at the UW in the late '40s. There, he was greeted by several reporters wishing to get the first interview with the son of Sir Hans Krebs...
When I arrived as a postdoc in Seattle, I was thoroughly intimidated by this great man. Even the most innocuous conversations were awkward as I tried to get to know him. Then, one evening when the lab was quiet, I was just finishing a cartoon I had drawn that depicted one of my fellow postdocs, Don Tinker, dressed in a tutu, wielding a magic wand. Ed walked past me on his way out. Behind me, I heard him softly repeat the words of the caption— "Tinker Bell"— followed by a quiet chuckle. I had discovered Ed's dry but wicked sense of humor.Peter Kennelly
Professor and head of biochemistry
Virginia Polytechnic Institute and State University
My postdoctoral experience with Ed Krebs molded my scientific career. Ed was a very kind gentleman. He had a special knack for telling you that your ideas or interpretations were wrong in an unobjectionable way. He was careful to not overtly dictate any of his ideas to his scientific group, but he made suggestions and nourished his thoughts carefully in order to guide young scientists toward the right goals. Sometimes, I was proud to have a good idea, but I came to realize later on that the idea actually came from Ed. I remember just a few times that he was persistent in directing my research toward a specific direction, but, even then, he did it in a congenial manner, and he was practically always right.
Ed will be sorely missed in the scientific world, especially in the field of signal transduction. I miss my wonderful mentor and good friend.
Jackie D. Corbin
Professor of molecular physiology and biophysics
Vanderbilt University Medical Center
For me, Ed was a hands-off adviser. He gave people tremendous freedom— there was certainly ample opportunity to sink or swim in his lab. Ed had a heavy journal editor role, so we did not see a lot of him in the laboratory, but every now and then he would drift in for coffee. The one time Ed came into the laboratory on a Saturday, we were so pleased to see him and be noticed for our diligence, but it came with an unexpected cost. He was there to centrifuge some terrible homemade wine, and we had to taste it in little glass beakers.
Between his hearing aid and my Australian accent, I am not sure he understood a thing I said for the first six months. When I first met Ed, I was struck by his being so low key and devoid of any "brilliant façade," but then I came to realize that nothing but clear thinking and common sense ever parted from his lips. While somewhat remote, he was immensely likeable, someone whose value just kept growing on you.
His weekly laboratory meetings were of tremendous value and provided the real mentoring environment. Ed was keenly interested in politics and the U.S. economy. He was a strong critic of the infamous U.S. Sen. William Proxmire and his "Golden Fleece" awards. Perhaps the best illustration of his political interests was at a plenary lecture at a Gordon Conference, where he traced the development of the protein phosphorylation field over his long career and what was happening in politics in the U.S. at the time of each major scientific development.
We remember Ed with fondness and enduring thanks for the impact he had on science and our lives.
Bruce E. Kemp
Pehr Edman fellow in protein chemistry and metabolism
St. Vincent's Institute of Medical Research
My time with Ed Krebs as a postdoc in the mid-1970s in both Davis and Seattle had a great influence on my scientific development. Ed had a "hands-off" approach to science that focused on providing resources for a large lab with mostly postdocs, each with a different, mostly self-directed project that had minimal overlap with other projects. This freedom had a broadening impact on postdocs, and, for myself, it formed the model for my own laboratory. Ed expected each person to have passion for his or her work and once commented that he was disappointed to drive by the lab late on a Saturday evening and find no lights on. We postdocs then decided to leave the lights on 24/7.
Ed was a traditional "ice bucket biochemist" and enzymologist, and I was his first postdoc with a cell biology background. I think he was initially not sure what to make of "biochemistry without a license," but that skepticism vanished when we were quickly able to show by microinjecting protein kinase A into Xenopus oocytes that changes in kinase activity could fully account for all the in vivo actions of cAMP in an important biological process. For myself, I learned how to purify enzymes, beginning with phosphorylase kinase, from kilograms of tissue, and this experience served me well in later years when purifying mitotic enzymes (Cdks), whose characterization had eluded others for many years. It was an amazing time in science, because, although Krebs and Fischer had shown the critical importance of phosphorylation in glycogen metabolism, it was not thought to be of much importance for other aspects of biology. At the end of my postdoc, it was reported that the Src oncogene encoded a protein kinase— a finding that linked kinases to cancer and ultimately led the way to the current realization that protein phosphorylation controls virtually all biological processes. It was one of those rare times in science when one happens to be working on something that turns out to be much more important than initially believed.
Although Ed received many honors during his lifetime, his gentle nature was very unassuming, and he exhibited little interest in scientific politics or in going to meetings. He had a long-standing presence in ASBMB as associate editor of the JBC and felt that there was no need to search for "higher impact" journals if the work was sound. Throughout his life, he kept focused on key questions in glycogen metabolism, even after vastly increased support from Howard Hughes Medical Institute and winning the Nobel Prize. To the end, he felt that there were many questions still unanswered about the regulation of metabolism by protein phosphorylation, and he wanted to continue working on them.
James L. Maller
Professor of pharmacology
University of Colorado School of Medicine
I was fortunate to have Ed as my Ph.D. mentor from 1966 to 1970. Two specific pieces of advice that influenced my career come to mind. The first was when I was a student in Ed's lab and was concerned that another lab was working on the same project. Ed's advice was that any time you are working on an important project, there will be strong competition, so find some niche where you have an advantage and pursue that aspect. The second was as a professor at Vanderbilt (University) when we were both Howard Hughes investigators. There had been a change in Hughes leadership, and I was concerned about whether or not they would continue to fund my research. Ed told me to not worry about what Hughes wanted, just make sure my research was outstanding— if so, it would all work out. He was right! Ed's legacy will continue through the many young scientists he mentored.
Tom Soderling
Senior scientist, Vollum Institute
Professor of biochemistry
Oregon Health and Science University
Thinking of Ed always brings a smile to my face. While others will rightfully comment on his brilliant scientific insight, his administrative skills and his role as a mentor, what particularly stands out in my memory is Ed's unusual sense of humor. About 10 years ago, I brought a star-struck graduate student to meet Ed, who proceeded to talk to her about research that he had done "during World War I." Afterward, he professed to me his amusement that the student had not caught the historical error. This, and many other incidents, always left me wondering whether Ed was kidding around in a given situation. He kept such a straight face that one could never tell. Once, during a whiskey-laced late-night card game at one of our Pack Forest retreats, Ed told me that I had a good poker face. Coming from him, this was quite a compliment!
I thank Ed for many things, including his anecdotes of agricultural pursuits, his tutelage in antique hunting and his mentoring in how to write manuscript reviews, but most of all I thank him for demonstrating how a great scientist can be a great human being.Kathryn E. Meier
Director of program in nutrition and exercise physiology
Washington State University
It is a pleasure to remember Ed Krebs as a friendly and passionate teacher and science supervisor. His pioneering work on protein kinases as modulators of cellular functions served as a basis for my later investigations. He cordially shared his knowledge of biochemical methodology and readily participated in discussions on protein kinases and protein phosphatases. Much of what I and my colleagues were able to accomplish was made possible by his intuitive research.
Franz Hofmann
Emeritus professor of pharmacology
Technische Universität München
I was on a plane from Durham, N.C., to Seattle, Wash., to start my postdoc in Ed's lab when Mount St. Helens first erupted. It was an apt introduction to my time in Ed's lab. It was May 1980, and tyrosine phosphorylation of pp60src recently had been described. There followed a rapid explosion in our knowledge of protein tyrosine kinases, and Ed's lab was the ideal place to be during this revolution. Ed was supportive of his postdocs who ventured into this new area, and he helped us apply the knowledge he had acquired in decades of studies on protein kinases to these new members of the family. He was rigorous in his approach to data but kind and patient at the same time. He was a wonderful mentor to whom I always will be grateful.
Linda J. Pike
Professor of biochemistry and molecular biophysics
Washington University School of Medicine
When I began working with Ed, his only directive was "find a protein kinase cascade— I know it exists." This initiated studies that led to the discovery and characterization of MAP kinases and MAP kinase kinases. I could not have become a professor without Ed's generosity in accepting me in his lab when my options were limited.
One of the most important lessons that Ed taught us by example was how to conduct ourselves as citizens within the scientific community. Our work on various projects led to competitive interactions with scientists at other institutions and sometimes within our own lab. Ed maintained a balanced approach to scientific competition, treating whomever he met as a colleague and showing him or her great respect and kindness. There were very few people who Ed disliked, and he always was able to find strengths in every person. I recall sessions in his office when Ed pulled out letters and other documents from his past that illustrated in various ways "how to handle oneself with aplomb." Ed valued this deeply, and, in doing so, he set a high standard for fairness, collegiality and professional conduct, which continues to influence investigators throughout the field of signal transduction.
What I remember most fondly about Ed was his keen sense of humor. We'd have to pay close attention in order to pick up his hilarious observations, which were often delivered as soft, understated commentaries, as well as the occasional practical joke. The year was 1991 and Bill Clinton had generated controversy during the primaries with statements about his drug use. To kick off the annual Krebs & Fischer lab retreat at Mt. Rainier, Ed stood up and confessed to the group that "I too have used pot, but that was before I became a professor."
Natalie Ahn
Associate professor of chemistry and biochemistry
University of Colorado at Boulder
Ed Krebs was one of those people whose accomplishments were so numerous and so extraordinary that, paradoxically, they were easy to overlook because they formed such a huge part of our scientific world. But, every time we teach or do research on the regulation of pretty much anything that goes on inside the living cell, the chances are good that we are either talking about or building on his work.
Gregory A. Petsko
Gyula and Katica Tauber professor of biochemistry and chemistry
Brandeis University
I was privileged to work for and with Ed for nearly 40 years. During this time, I do not believe I ever heard him raise his voice to me in disapproval though there were plenty of times when I deserved it. Ed gave his students and faculty a huge amount of freedom and yet somehow always seemed to have his hand on the "pulse of the lab." He preferred to lead by example rather than by giving orders. Eventually we all learned that his gentle suggestions were something to which we needed to pay attention. Ultimately, we were all better off for the experience. He will be sorely missed.
Joe Beavo
Professor of pharmacology
University of Washington
1. Fischer, E. H., and Krebs, E. G. (1955) Conversion of Phosphorylase B to Phosphorylase A in Muscle Extracts. J. Biol. Chem. 216, 121 - 132.
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HTC One X sold early at AT&T, spotted in the wild
HTC's hotly anticipated One X isn't supposed to ship to AT&T customers until May 6th, but there's a reason we're emphasizing the whole "supposed" thing. One lucky reader has managed to convince one particular store to sell them a handset early, with $199 (and a new two-year agreement) being all it took to grab hold of what'll undoubtedly be one of 2012's strongest Android phones. There's a gallery of shots below as proof, and if you'd like to try your luck at a local outfit, we certainly won't step up to stop you. (But look, you'll probably be waiting a few more weeks -- just sayin'.)
[Thanks, Anonymous]
Gallery: HTC One X sold early through AT&T
HTC One X sold early at AT&T, spotted in the wild originally appeared on Engadget on Mon, 23 Apr 2012 21:28:00 EDT. Please see our terms for use of feeds.Permalink | | Email this | Comments


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Jump to Content
Series 1 Correspondence, 1977-1990
Bentsen, Lloyd (U.S. Senator), August 1, 1977
Boston Lesbian and Gay History Project, January 26, 1980
Interrante, Joe
Kelly, Beth
Brown, Harold, [U.S.] Secretary of Defense, 1977
Huggins, Lewis H., [U.S.] Department of the Army
Burg, B. R.-–See: Box 2 / Folder 9 Publications series
Committee on Homosexuality in History, 1979
Sprague, Gregory
Crompton, Louis, 1978-1979
Monahan, James
DeCecco, John P.-–See: Box 1 / Folder 10 Journal of Homosexuality
Duberman, Martin, 1978
Gilbert, Arthur N., March 9, 1979
Journal of Sex Research
Hansen, Bert, 1980-1981
Huggins, Lewis H.-–See: Box 1 / Folder 3 Brown, Harold
Interrante, Joe-–See: Box 1 / Folder 2 Boston Lesbian and Gay History Project
Journal of Homosexuality, 1979-1980
DeCecco, John P.
Kellogg, Stuart
Journal of Sex Research-–See: Box 1 / Folder 8 Gilbert, Arthur N.
Just Before Dawn (radio show)-–See: Box 1 / Folder 17 Whitley, J. B.
Katz, Jonathan, 1977-1980, 1983
Kelly, Beth-–See: Box 1 / Folder 2 Boston Lesbian and Gay History Project
Licata, Sal (Salvatore J.), 1977-1990
Monahan, James-–See: Box 1 / Folder 5 Crompton, Louis
Monter, William, March 10, 1979
Ron (Pergamon Press), 1986-1987
Sprague, Gregory A.-–See: Box 1 / Folder 4 Committee on Homosexuality in History
U.S. Department of the Army--See: Box 1 / Folder 3 Brown, Harold
Just Before Dawn (radio show)
Williams, Walter, June 1979
Series 2 Subject Files, 1950-1995 Bulk, 1977-1992
Clippings, 1977, 1992
Connecticut and New Haven,
New Jersey
New York
North Carolina
Katz, Jonathan
Rhode Island and New Hampshire
South Carolina
Dallas Gay Political Caucus, 1978
Gay Academic Union, San Francisco Bay Area Chapter, 1978-1980
Kepner, James (Jim), 1975-1976
Kinsey, undated
Mattachine Society--See: Box 1/ Folder 6 D'Emilio, John
McCarthyism, excerpts from Congressional Record, 1950
Miscellaneous history, 1975-1995
National Gay Archives, undated
ONE (Dorr Legg), 1962-1977
Protect America's Children-–See: Box 1 Folder 19 Bryant, Anita
17th and 18th Century Sexuality—Miscellaneous, undated
Series 3 Reprints and Photocopies of Printed Articles, 1908-2001 Bulk, 1976-1978
Bentham, Jeremy, 1978
"Jeremy Bentham's Essay on 'Paederasty': an introduction" by Louis Crompton. ( Journal of Homosexuality, vol. 3, no. 4, pp.383-387), Summer 1978
"Offences Against One's Self: Paederasty. Part 1" by Jeremy Bentham, edited by Louis Crompton. ( Journal of Homosexuality, vol. 3, no. 4, pp.389-405), Summer 1978
"Jeremy Bentham's Essay on 'Paederasty.' Part 2" by Jeremy Bentham, edited by Louis Crompton. ( Journal of Homosexuality, vol. 4, no.14, pp.91-110), Fall 1978
Bingham, Caroline. "Seventeenth-Century Attitudes Toward Deviant Sex" by Caroline Bingham. ( Journal of Interdisciplinary History, vol. 1, no.3, pp.447-472), Spring 1971
Bullough, Vern L. "Challenges to Societal Attitudes Toward Homosexuality in the Late Nineteenth and Early Twentieth Centuries" ( Social Science Quarterly, vol.58, no.1, pp.29-44), June 1977
Bullough, Vern L. "Sex Education in Medieval Christianity" ( The Journal of Sex Research, vol.13, no.3, pp.185-196), August 1977
Burg, B. R., 1977-1978
Letters from B. R. Burg to Robert Oaks, March 1, 1977; September 1, 1978
"Pirate Communities in the Seventeenth Century: A Case Study of Homosexual Society" [by B. R. Burg], [1977?]
"Ho Hum, Another Work of the Devil: Buggery and Sodomy in Early Stuart England" [Presentation paper by B. R. Burg], [1978]
"Chapter I. Sodomy and Public Perception: Seventeenth-Century England" [by B. R. Burg], 1978
Burnham, John. "Early References to Homosexual Communities in American Medical Writings" ( Medical Aspects of Human Sexuality, (August 1973), pp.34-49), 1973
"The Death Penalty in Colonial America" [1625-1873, one-page chronology /photocopy of typescript], undated
"Homosexuals and The Death Penalty in Colonial America" ( Journal of Homosexuality, vol. 1, no.3, pp.277-293), 1976
"Lesbianism and the Law" [342-1950, one-page chronology / photocopy of typescript], undated
Flaherty, David H. "Law and the Enforcement of Morals in Early America" ( Perspectives in American History, vol.5 (1971): "Law in American History," pp.203-253, edited by Donald Fleming and Bernard Bailyn), 1971
Forgey, Donald G. "The Institution of Berdache Among the North American Plains Indians" ( The Journal of Sex Research, vol. 11, no. 1, pp.1-15) February 1975
Garner, Brian and Richard W. Smith. "Are There Really Any Gay Male Athletes? An Empirical Survey" ( The Journal of Sex Research, vol. 13, no. 1, pp. 22-34), February 1977
Gilbert, Arthur N. "Buggery and the British Navy, 1700-1861" ( Journal of Social History, vol. 10, no.1, pp.72-99), Fall 1976
Gilbert, Arthur N. "Sexual Deviance and Disaster During the Napoleonic Wars" ( Albion, vol. 9, no. 1, pp.98-113) Spring 1977
Hansen, Bert. "The Historical Construction of Homosexuality" ( The Radical History Review, no. 20), pp.66-73, Spring/Summer 1979
Hindus, Michael S.-–See: Box 2 / Folder 28 Smith, David Scott
Hooker, Evelyn. "The Adjustment of the Male Overt Homosexual" ( Journal of Projective Techniques, vol.21, pp.18-31), 1957
Hooker, Evelyn. "A Preliminary Analysis of Group Behavior in Homosexuals" ( Journal of Psychology, vol.42, pp.217-225), 1956
MacDonald, Robert H. "The Frightful Consequences of Onanism: Notes on the History of a Delusion" ( Journal of the History of Ideas, vol. 28, pp.423-431) 1967
McIntosh, Mary. "The Homosexual Role" ( Social Problems, vol. 16, no. 2, pp. 182-192). 1968
May, Geoffrey. "In Pre-Revolutionary America" ( Social Control of Sex Expression (New York: William Morrow and Co., 1931), Chapter 12, pp. 229-255), 1931
Oaks, Robert F. "Perceptions of Homosexuality by Justices of the Peace in Colonial Virginia"--See Box 2 / Folder 27 Sexual Law Reporter, vol. 4, no. 2
Oaks, Robert F. "'Things Fearful to Name' : Sodomy and Buggery in Seventeenth-Century New England" ( Journal of Social History, vol. 12, no. 2, pp. 268-281), undated
Smith, David Scott & Michael S. Hindus. "Premarital Pregnancy in America 1640-1971: An Overview and Interpretation" ( Journal of Interdisciplinary History, vol. 4, pp. 537-570), Spring 1975
Smith, Richard W.-–See Box 2 / Folder 15 Garner, Brian
Stark, Gary D. "Pornography and the Police in Imperial Germany, 1870-1914," undated
Trumbach, Randolph. "London's Sodomites: Homosexual Behavior and Western Culture in the 18th Century" ( Journal of Social History, vol. 11, pp.1-33.) 1977
Westermarck, Edward. "Homosexual Love" from The Origin and Development of the Moral Ideas (London: Macmillian and Co., 1908), vol. 2, Chapter 43, pp.456-489. 1908


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text:
How to use the iPad Online User Guide
The iPad online User Guide is similar to the Help system on a Windows or Mac computer. The online help guide is accessed through the Safari browser, as a bookmarked site.
Use the online User Guide to learn how to use your iPad, or to answer a quick question. For example, if you're curious about how to use Photos, the iPad User Guide is a great place to start. There, you'll soon learn how to take photos and videos, display your photos in a slideshow, or stream a video onto your HDTV.
Access to the iPad User Guide is quick and easy since it's right there on your iPad.
1From the iPad Home screen, tap the Safari icon.
The Safari web browser starts up.
2Tap the Bookmark icon. On the Bookmarks menu that appears, tap iPad User Guide.
The iPad User Guide is displayed in the browser window.
3Tap a topic on the left.
A list of applicable subtopics is displayed on the right side of the screen.
4Tap a subtopic.
Information about the selected subtopic is displayed. Tap any link in the subtopic information to access additional topics.
5Tap the Home screen button.
The browser is closed.
You can also access the iPad User Guide on your computer, through its web browser if you like.


text:
Ten Thousand Things
Your search returned no results.
Click here to clear all filters.
Click here to clear all filters.
View all the items you have purchased online and in-store in your Virtual Closet, check your alteration status, track online orders, and communicate with your Style Advisor.Log in
Don't have an account? Create an account
When you select UPS 2nd Day at checkout and place an order by 12pm EST on Dec 18


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In 2011, IBM released three new data replication products:
- IBM InfoSphere Data Replication (IIDR), v10
- InfoSphere IMS Replication for z/OS, v10
- InfoSphere Classic Change Data Capture for z/OS, v10 (for use with IMS)
One question that comes up is whether the two IMS replication products are compatible with either the new Data Replication product or the existing InfoSphere CDC products. The answer is yes - the IMS products are compatible with both new and existing products that contain the CDC technology. IMore specifically, they can provide IMS changed data to any data replication solution that you can build with IBM's CDC technology. For example, you can create unidirectional (one-way) subscriptions that feed IMS changed data to any database that can be targeted by CDC:
Two notes about this picture:
- IBM recommends you use the CDC technology in IIDR if you do not own InfoSphere CDC.
- The target DB2 can be DB2 for z/OS, DB2 LUW, or DB2 for System i.
You could also feed IMS changed data into other business software such as ETL, IBM's DataStage, and ESBs:
In other words, the new IMS data replication products extend the reach of IBM's CDC technology by adding IMS as a source for log-based capture of changed data. If you have technical questions, see the Classic CDC section of the Information Center.


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AsiraMuslim video.AsiraMuslim webcam
I like to be as different from everyone else as I possibly can because individuality barely exists anymore. I have been told I can be a people pleaser, but I`ve also heard I`m clever and feisty with a sarcastic tongue. ...i can be your angel or your evil


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What is Museum 2 You
Welcome to the beginning of the Museum 2 You program, I just thought I'd drop in and introduce myself. I'm Jon, and I'm an Intern working on the project with Karen. I'm really excited to get this program up and running.
Karen has mentioned we have our survey up and running at the moment for you to have your say on what goes into the displays for the Museum 2 You project. We have had a great response so far but we want your input; if you have already responded and are reading this, thank you very much for your contributions they are greatly appreciated. If you'd like to contribute but haven't yet, just head off to this link: http://www.surveymonkey.com/s/YLQQSKN
You will see me around here on a regular basis making posts and encouraging discussion. If you have any questions or comments you can post them up here, don't forget to also visit the Museum 2 You website at: http://www.australianmuseum.net.au/Museum-2-You where you can also leave comments for us as well. There is a lot of great information there for you as well as some practical information to download like our bug collecting manual to get you started.
So, why don't I start a discussion, and it's a question that is both really quite simple and yet very complex at the same time; What does biodiversity mean to you? To find out more about what Biodiversity is, why not head over to the Museum 2 You page and see what we have there; http://www.australianmuseum.net.au/What-is-biodiversity once you've had a read of that, pop back over here to the blog and tell us what you think of when you consider what Biodiversity means to you.
Speaking of Biodiversity, don't forget that 2011 is the International Year of Forests in Australia as well. http://www.internationalyearofforests.com.au
I look forward to reading your responses and also bringing this project out to you.


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Dinner Tonight: Proper French Omelet Recipe
Your source for quick recipe every single week day.
This post isn't meant to be a take-down of good old American diner omelets: fluffy, stuffed full of fixings, oozing cheese. I happen to love omelets like that. But I also love an omelet that's done in the classical French way, a more artful affair that's thin, spare, and just barely cooked past runny. Laced with a bit of cheese, perhaps some chopped herbs, or nothing at all—what's important is that the omelet is not a vehicle for what's inside. It's about the eggs themselves.
And it's a sublime transformation from raw eggs to omelet, done in less than two minutes (unlike scrambled eggs, which are best cooked for an age over impossibly low heat), so the perfect quick pantry dinner. I found the recipe I was looking for in an old post on eGullet, a website which never fails to satisfy (and usually stoke) my curiosities. Besides offering good suggestions for salt and pepper, it also suggests a teaspoon of cold water per egg, which steams during cooking, adding some extra fluffiness to the eggs.
In the end, a good omelet is about good technique, which is really a matter of practice. But this post got me started.
- Yield:1
- 2 tablespoons butter
- 2 eggs
- 1/4 teaspoon salt
- 4 grinds of fresh pepper (preferably white, but only for aesthetic reasons)
- 2 teaspoons cold water
- 1/4 cup grated cheese (optional)
- 2 tablespoons chopped fresh herbs (optional)
In a mixing bowl or measuring jar, combine the egg, seasoning, and water. Whisk vigorously to combine so that the mixture is relatively homogeneous.
Heat a small skillet, preferably nonstick, over medium-high heat until drops of water dance on the surface. Add the butter. When the foam subsides, add the egg mixture all at once.
Using a spatula, fold one side of the omelet 1/3 of the way towards the opposite side. Tip the omelet out of the pan, rolling it onto a plate so both sides fold under. Serve immediately.
This Recipe Appears InQuote of the Morning: 'Why Add Water to Eggs?'
All products linked here have been independently selected by our editors. We may earn a commission on purchases, as described in our affiliate policy.


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LAST ONE IM RETIRED (now if anyone wants to make one of lil' ol' me.....? :D )
For Klyde, I felt bad and came out of retirement for him. Rainbow hair, I remember he dyed his hair real cool once, and the "baka" in honour of Photon who wrote it on everyone (no, I don't mean to say you're an idiot, honest) ... I thought I was the only one who ever watched it (loved the music). ...and famous ellipses. ;)