text: Home Seminars Poster Printing Info Report Form and Instructions Poster Critique Form Biomedical Sciences Seminar Series Spring 2008 Poster PresentationMay 2, 2008 at 1 pm Physical Sciences Lobby Roxana Rangel Rodriguez, Monica Delgado, Morio Gallardo, Maura Palacios, Sergio Rivas, Nadine Rodriguez, Elizabeth Alusha, Cindy Barlan, Ann Marie Valenzuela, Angelina Hernandez, Abebayehu Yilma, Lorilee Tallorin, Michelle Flores, Yesenia Velazco, Joey Contreras, Ricardo Sanchez, Cari Wilson, Suzi Sanchez, Ivy Donaldson Poster #1- Roxana Rangel Rodriguez Cation-p Interaction in Small α-Helical Peptides Roxana Rangel-Rodriguez, Dr. Alison McCurdy. Cation-p interactions have been shown to play an important role in biological systems. The objective of this research is to gain a better understanding of the stabilizing non-covalent cation-p interaction in model helical peptides. The cation-pi interaction in small alanine-based a-helical peptides has been studied by CD spectroscopy and the cation-pi interaction has been quantified by calculating the free energy of stabilization. Pairs of residues Cha/R, Cha/K, F/K and F/R were investigated with four and five residues in between. This data was added to previously determined data for pairs of residues Y/R, Y/K, W/K and W/R. The free energy of stabilization for all peptides was examined and the uncertainty on these values was found. top Poster #2-Monica Delgado Use of Site Directed Mutagenesis and Reverse Genetics to Create a Variant of Sendai Virus, RGV22, to Study the Effects of a Mutation in the L Protein on the Rate of Virus ProductionMonica Delgado M.S Prospectus The emergence of new viral human pathogens from variants of viruses that infect other animals has highlighted the importance of investigations on the genetic basis of viral infections. The long-term goal of this lab is to understand how the structure and function of Sendai virus proteins relate to viral pathogenicity. Wild-type (wt) Sendai virus causes a localized respiratory tract infection in mice, while a mutant, F1-R, causes a systemic infection. We attributed he difference in type of infection to amino acid changes in the F and M proteins of F1-R. In tissue culture, wild-type virus consistently produces at least 10-fold more viruses than F1-R. Since M is known to be a negative regulator of viral mRNA synthesis, we attributed the difference in virus production rate to the amino acid changes in the M protein of F1-R. However, studies with reverse genetics viruses generated to identify the amino acid substitutions necessary for the systemic behavior of F1-R proved that amino acid substitutions in F1-R M are not responsible for F1-R's reduced rate of virus production. RGV0, a reverse genetics virus that contains all F1-R F and M amino acid changes produced at least 10-fold more viruses in tissue culture than did F1-R. In mice it caused a systemic infection and preliminary evidence suggests that it is more virulent than either F1-R or wild-type Sendai virus. We suggest that the increased virulence of RGV0 with respect to F1-R is due to the increased rate of virus production in the infected mice. F1-R has a single mutation in each of the P and L genes that encode the viral polymerase complex proteins. Amino acid changes in either or both of those proteins are likely to be the cause of the decreased rate of virus production and decreased virulence of F1-R. This study will test the hypothesis that the mutation in the L gene of F1-R causes the decreased rate of virus production and decreased virulence of F1-R. To address this hypothesis, we will use reverse genetics to construct a virus, which we will call RGV22, containing the L gene mutation of F1-R. The RGV22 production rate will be studied in tissue culture via a multiple cycle replication assay and the pathogenicity of RGV22 in mice will be studied using real-time PCR and plaque assays on organ homogenates from infected mice. top Poster #3- Mario Corona Creating cysteine sulfinic acid in creatine kinase with hydrogen peroxide Mario A. Gallardo and Jamil Momand Cysteine thiols in proteins are susceptible to oxidation. One molecule that has been shown to oxidize proteins is hydrogen peroxide. Hydrogen peroxide is generated in biological systems and has been shown to oxidize protein cysteine thiols to disulfides, sulfenic acids and sulfinic acids. Oxidized proteins possess altered activities. One protein that is susceptible to hydrogen peroxide-mediated oxidation is muscle creatine kinase. It is possible that oxidized creatine kinase is a target for degradation or repair. To test this hypothesis an oxidized form of creatine kinase was created. Hydrogen peroxide treatment of creatine kinase decreased the pI of the protein from its natural pI of 6.1. Two more acidic forms of the protein were created after hydrogen peroxide treatment as determined by isoelectric focusing. top Poster #4-Maura Palacios The influence of varying salinity on spatial patterns of larval settlement of the mussels Mytilus californianus and Mytilus trossulus in Barkley Sound, British Columbia Maura Palacios M.S. Biology Faculty Mentor: Dr. Torres/ Dr. Robles A commonality seen along the pacific coast of North America is the alongshore segregation of closely related mussel species. Beds of Mytilus californianus are restricted to wave-beaten headlands, whereas M. trossulus aggregations occur on shores protected from wave action. As a classical topic that has challenged the Marine Ecology community for years, current theory refers to post-settlement mortality as the cause. M. californianus smothers M. trossolus on the headlands and in quiet bays the effects of siltation suffocate M. californianus eliminating the overgrowth effect. The proposed method is applicable in certain regions of the pacific coast but irrelevant in the Pacific Northwest where silt accumulation is not observed. The proposed theory for this study is salinity tolerance plays a crucial role in the observed phenomenon. Specifically, salinity tolerance for larvae of M. trossolus is wide but M. californianus isnarrow. Also, abundance in the water column and settlement rates of both types of larvae is high on open coastlines due to wave action and vertical mixing maintaining a constant salinity. Field and laboratory experiments proposed would develop reliable identification based on morphology, confirm differential tolerance of the larvae, describe spatial patterns of incursions, and determine rates of survival during incursions. top Poster #5-Sergio Rivas The study of expander graphs that are Ramanujan.Sergio Orlando Rivas MS Mathematics Faculty Mentor: Dr. Shaheen, Anthony The theory of expander graphs is a hot topic in mathematics and computer science, as these graphs have a wide range of applications. One of these applications is to communications networks. Think of a communications network as a graph where each vertex represents a computer in a network and two vertices are connected by an edge if they can communicate directly with one another. When designing a communications network one wishes that any message originating from anywhere within the graph will propagate quickly. There are various measurements, which describe how well a graph spreads messages. One such measurement is called the spectral gap of a graph. A graph is k-regular if every vertex of the graph has k edges incident to it. The spectral gap of a connected, k-regular graph X is given by k– λ1(X), where λ1(X) is the second largest eigenvalue of the graph X. One wishes to construct infinite families of regular graphs where the spectral gap of each graph is bounded away from zero by a fixed amount. That is, one wants to make λ1(X) as small as possible. But at the same time one wishes to minimize the number of edges in each graph of the family. Some of the best graphs that have the property of being highly connected are families of Ramanujan graphs. In this study we will start by investigating the properties of Cayley graphs, Cay(G,S) , which encodes the structure of a group G when a generating set S is given. We want to know what types of groups G, along with their generating set S, give rise to families of Ramanujan graphs. top Poster #6-Nadine Rodriguez Response of Paneth Cells in Small Intestine of Mice to Salmonella enterica serovar Typhimurium Nadine Rodriguez and Dr. Edith Porter Innate mucosal immunity represents the first line of defense preventing most microbes from entering the gut lining and invading the bloodstream. Paneth cells (PC) located at the base of the small intestinal crypts contribute to the mucosal first line defense by the secretion of granules filled with antimicrobial polypeptides. Recently interleukin 17 (IL-17) has been discovered as inflammatory cytokine regulating epithelial cells in the intestine. Previous studies indicated that PC in mice infected with Salmonella enterica serovar Typhimurium (ST), one of the leading causes of gastrointestinal infections in humans, contain less granules than PC in control mice. Therefore, we undertook to enumerate the granules in PC in hematoxylin & eosin stained small intestinal tissue sections obtained from mice one and three days after inoculation with ST or buffer only. There was a statistically significant decrease in the number of granules per PC in ST infected mice after three days accompanied by an increase in the number of PC per crypt. The total number of granules within the crypts was not significantly altered. Furthermore, we observed an increase of mitotic events in ST infected tissue sections. In addition, we found one day after inoculation increased IL17 levels in the tissue extracts from mice infected with ST compared to tissue extracts from control mice. However, IL17 levels were reduced in ST infected mice three days after inoculation. These data suggest an accelerated production of PC with the reduced number of granules reflecting immaturity as part of the host response which may be mediated by IL17. Our study may contribute to a better understanding of the host response to Salmonella infections. top Poster #7-Elizabeth Alusha What Turns People Off: A Descriptive Study of Sexually Repellent Features Elizabeth Alusha, B.A. and Pamela Regan, Ph.D., Department of Psychology, California State University, Los Angeles, Los Angeles, CA. One of the unfortunate truths of human existence is that we cannot always obtain the kind of partner we ideally desire. Yet compromise and choice, which are important aspects of any mating relationship, generally are not considered by theorists or researchers in the areas of attraction and mate selection. Rather, the majority focus on what people ideally seek to obtain in a potential partner (i.e., on their unmoderated, unadulterated desires). The present descriptive study addressed this limitation by exploring beliefs about sexually undesirable partner attributes (that is, those features that exclude someone from the field of potential short-term mates). Adults (N = 900; 450 men and 450 women) indicated in a free response format the features that they definitely would not want in a short-term sexual partner. Participants specified a number of sexually undesirable partner attributes related to appearance (e.g., body weight, poor skin; 80.1%), personality (e.g., emotional instability, lack of intelligence; 70.1%), hygiene (e.g., poor general hygiene; 45.3%), sexuality (e.g., inexperience, aggression; 39.8%), social status (e.g., illiteracy; 21.0%), relational status (e.g., committed to another; 14.8%), bad habits (e.g., drug or alcohol use; 14.2%), and health (e.g., AIDS; 12.0%). This result pattern indicates that the characteristics that men and women find sexually repellant (e.g., unattractiveness, emotional instability, stupidity) are the opposite of those they find particularly sexually appealing, and lends support to social contextual and evolutionary models of human mating. In addition, few sex differences were found. In particular, the strong and equal emphasis that men and women placed on undesirable physical attributes supports a growing body of evidence that appearance attributes play an important role in the short-term mating decisions of both sexes (see Gangestad, 1993; Regan, 2003). Interestingly, although men and women specified similar characteristics, women generated significantly more repellant features than did men (p < .001). This finding is in accord with theory and research that suggests that women have higher standards than men in the context of short-term mating relationships (Regan, in press). top Poster #8- Cindy Barlan Alteration of Serum Enzyme Kinetics by Dietary Jojoba Oil Maintains Normal High Density Lipoprotein Cholesterol Concentration in Cholesterol-Fed Rabbits Cindy Barlan, Susie Rascon, Christine Do, and Raymond E. Garcia Abstract: Most cardiovascular diseases (CVD) are caused by the formation of atherosclerotic plaques in the arteries. High density lipoprotein total cholesterol concentration [HDL-C] is considered an anti-atherogenic determinant in diagnosing the risk for CVD. New Zealand White (NZW) rabbits fed a cholesterol-rich diet exhibit a decrease in [HDL-C], whereas those fed a cholesterol-rich diet supplemented with jojoba oil maintain normal [HDL-C]. The objective of this project is to determine the mechanism behind these observations by studying the kinetics of the enzymes involved in the reverse cholesterol transport pathway. We hypothesize that dietary jojoba oil in the presence or absence of dietary cholesterol increases lecithin:cholesterol acyltransferase (LCAT) and decreases cholesteryl ester transfer protein (CETP) activities. Blood samples were obtained before and after feeding NZW rabbits either normal (N), 3% jojoba oil (J), 1% cholesterol (C), or, 1% cholesterol + 3% jojoba oil (CJ) diet for 7 days. Total cholesterol (TC) and free cholesterol (FC) concentrations were determined using enzymatic assays. LCAT rate was determined by measuring the disappearance of FC over a three hour period. LCAT rates in μmol/dL/h were: CJ = 217.8 ± 14.2 > C = 157.3 ± 22.8 > (J = 37.9 ± 2.3 ≈ N = 40.4 ± 2.9). CETP rates in μmol/dL/h were: C = 63.9 > (CJ = 45.4 ≈ J = 41.1 ≈ N = 34.3). These results indicate that dietary jojoba oil stimulates LCAT and inhibits CETP whenever cholesterol is present in the diet. (Supported by NIH MBRS-RISE Grant R25 GM61331.) top Poster #9- Ann Marie Valenzuela Parental marital conflict and divorce, parent-child relationships, social support, and relationship anxiety among Latinos in young adulthood Ann Marie Valenzuela & Heidi R. Riggio, Ph.D. Previous research indicates that attributes of the family unit are related to quality of relationships in young adulthood, including relationships with parents and siblings, and overall perceptions of social support (Riggio, 2001, 2004). Parental divorce and the experience of parental marital conflict are especially likely to influence features of relationships with parents and beliefs about the self in relationships with others, including feelings of anxiety in relationships (Amato & DeBoer, 2005; Riggio, 2004). The current study investigates the harmful consequences of parental conflict and divorce for adult Latino offspring. A sample of 180 young Latino adults from divorced and intact families completed self-report measures of parental conflict (Peterson & Zill, 1986), quality of parent-adult child relationships (Kenny, 1987), anxiety in relationships with others (Snell, 1998), and perceptions of social support from others (Sarason, Levine, Basham, & Sarason, 1983). I hypothesize that divorce and conflict will have negative effects on the quality of relationships with both mother and father, perceived social support from others, and anxiety in personal relationships. In addition, based on previous research (Riggio, 2004), I expect parental divorce to be associated with higher quality relationships with mothers and lower quality relationships with fathers. top Poster #10-Angelina Hernandez STRESS EFFECTS ON HIPPOCAMPAL-DEPENDENT SPATIAL LEARNING: CAN EXERCISE PREVENT STRESS-RELATED BEHAVIORAL EFFECTS? Angelina Hernandez and Amelia Russo-Neustadt Chronic stress is known to have detrimental effects on the structure and function of the hippocampus. This brain region plays an important role in spatial learning tasks. Furthermore, glucocorticoids, or corticosterone in rats, are hormones secreted during times of stress that impair spatial learning (Roozendaal et al., 2003). In addition, it has been suggested that exercise can prevent the damaging effect of stress on the hippocampus. This may occur through one mechanism involving an upregulation of brain derived neurotrophic factor (BDNF) during exercise (Russo-Neustadt, 2003). In this study, it is hypothesized that stress impairs spatial learning and that this impairment can be prevented by the use of exercise. The model for stress used in this experiment is the chronic unpredictable stress model that consists of seven stressors: 24 hr illumination, 4 ºC forced swim, 24 hr food deprivation, 5 min tail suspension, 24 hr water deprivation, 20 min footshock, and 2 hr restraint. Animals will receive a different stressor each day for three weeks. Stressed and control rats will be tested on spatial learning ability using the Barnes circular platform maze. This maze is a circular maze with 18 evenly spaced holes around the edges. Under one hole, there will be a dark escape chamber with new bedding. Rats will use spatial cues, including bright posters and boxes, in a brightly lit room to locate the dark escape chamber hole. On the next part of this experiment, exercise will be implemented on a new cohort of control and stressed rats. The same behavioral test will be used to measure spatial learning ability. In support of the hypothesis, results displayed a longer latency time during testing to reach the escape chamber in the stressed rats compared to the control group. Furthermore, exercise improved latency times in the new cohort of stressed rats. In conclusion, the learning ability of stressed animals was impaired; however, with the use of exercise, the impairment in spatial learning was absent in stressed animals. These preliminary results can advance the knowledge of mechanisms involved in preventing neurodegenerative effects due to chronic stress. top Poster #11- Abebayehu N Yilma Human bronchioepithelial cell culture model to test the antimicrobial role of cholesteryl esters in innate host defenseµ Abebayehu N Yilma and Edith Porter Respiratory infectious diseases continue to be among the leading causes of illness and death world wide. A better understanding of natural host defense against microbial invasion may provide alternative strategies to prevent and treat infectious diseases. Previous research in our laboratory showed that host-derived lipids contribute to the inherent antibacterial activity of nasal fluid. In this study, we wish to demonstrate that cholesteryl esters contribute significantly to the inherent antibacterial activity of respiratory secretions employing an in vitro cell culture system with human bronchioepithelial cells. Our overall goal is to block the expression of Acyl-coenzyme A:cholesterol acyltransferase one (ACAT-1), a key enzyme of cholesteryl ester synthesis, and assess the effect on the antibacterial activity of these cells. Here, we report on the establishment of HBE4-E6/E7-C1 cells, the initial lipid analysis of their secretions and preliminary XTT assays targeted to evaluate the cytotoxicity of externally added lipids. We collected the supernatants from four day old submerged cultures and, for control, of unused culture medium. Total lipid extracts performed according to Bligh & Dyer were analyzed by TLC and rpHPLC. Our preliminary data suggests that HBE4-E6/E7-C1 cells secrete cholesterol and, importantly, cholesteryl esters in the low µg/ml range. Our preliminary cytotoxicity assays indicate that liposomal formulations of cholesteryl linoleate did not exert cytotoxic activity toward HBE4-E6/E7-C1cells. If successful this study may entail better treatment of chronic infectious diseases through manipulation of cholesteryl ester production. top Poster #12-Lori Tallorin Effects of Oxidative Addition via Singlet Oxygen on Pt (II) Thiolato Complex: A Model System Elucidating Nephrotoxicity by cis-Platinum Antitumor Drugs Lori Tallorin*, Dong Zhang*, and Matthias Selke* Keywords: Photooxidation; Persulfoxide; Metal Thiolate; Singlet Oxygen; Peroxide Effective treatment of nephrotoxicity by recognized cis- platinum antitumor drugs requires a mechanistic understanding of how these drugs initiate toxicity upon binding to cysteine-rich residues of metallothionein (MTs), a protein that is predominant in liver and kidneys and responsible for the detoxification of heavy metals. A study by Saryan et al. (1992) has shown that binding to cysteine-rich residues on MTs is cis-Pt(II) dependent implying that these Pt(II) cysteinato complexes are mainly responsible for nephrotoxicity. The exact mechanism of how these antitumor drugs induce toxicity is unclear. It is believed that oxidation of these metal bound MT cysteine residues may be one of the processes leading to severe side effects of cis-platinum drugs. The goal of this study is to determine whether short- lived peroxidic intermediate(s) are causing some of the damage. Studies by Gray et al. (1997) indicate the formation of the sulfinite product implying an intramolecular oxygen atom transfer upon photooxidation of a Pt(II) thiolate complex, implying intramolecular oxidation and no intermolecular oxygen atom transfer by the peroxidic intermediate. However, studies currently being conducted by our group indicate that peroxidic intermediates involved in the oxidation of the Pt-S-cys motif are in fact capable of intermolecular oxygen atom transfer and may therefore be involved in oxidative damage. The mechanisms of the peroxidic intermediate(s) produced by these Pt (II) cysteinato complexes may provide clues to understanding the mechanistic intricacies involved in the nephrotoxicity of these cis- platinum drugs. top Poster #13- Michelle Flores Examining Interracial Interactions through a Mixed-Methods Approach Kohatsu, Eric; Victoria, Rudolfo; Vasquez, Jason; Salazar, Andrea and Flores, Michelle Ongoing conflicts between Latinos and Asians in the U.S. contradict the much-adopted view that race relations in this country are harmonious and diversity-driven. This type of interracial interaction is best analyzed through a mixed methods approach in order to gain both a quantitative as well as a qualitative perspective (Kohatsu, 2007). The purpose of this study was to examine interracial interactions between Latinos and Asians using a mixed-methods approach. Participants were recruited from a culturally diverse west coast university and surveyed with established measures as well as an open-ended question. A thematic analysis revealed emerging themes through which participants identified their racial/ethnic group membership. Results further validated the use of mixed-methods in understanding the racial experiences of Asian Americans and other People of Color. top Poster #14- Yessenia K. Velazco Antimicrobial activity of liposomes against Neisseria gonorrhoeae Yessenia K. Velazco, Annie Cheung, Molecular Express, Inc., and Edith Porter. HIV infections continue to be of major concern worldwide. Previous studies have shown that sexually transmitted infections increase the HIV infectivity. We have recently shown that human defensin 5 HD5) is up-regulated during infection with Neisseria gonorrhoeae (NG), a fastidious gram-negative bacterium and one of the common causes of STIs. Our collaborators have subsequently shown that HD5 enhances the infectivity of HIV. Thus, prevention and better treatment of STIs may help to stem the HIV epidemic. Increasing resistance of NG to currently used antibiotics has been reported. In our laboratory we have developed novel antibacterial liposomes and our goal is to explore the activity of theses liposomes against NG. In this study, we describe our preliminary work to establish NG in our lab and to develop the best assay to determine anti-NG activity of antibacterial liposomes. We found that routine Mueller-Hinton media, routinely used for antibiotic activity testing, can be utilized for NG provided that the media s supplemented with isovitalex, a vitamin cocktail. We also found that we can use standard MIC testing with a modified read out to accommodate the particulate nature of liposomes. In MIC turbidity is used to measure bacterial proliferation. Instead we modified the read out introducing Syto 9, a quantitative DNA-binding fluoroprobe. Our next steps are to perform activity testing with our antibacterial liposomes. top Poster #15- Joey Contreras Chronic Unpredictable Stress reduces BDNF/mRNA Expression in the Rat Hippocampus Joey Contreras M.S. Biology Faculty Mentor: Dr. Amelia Russo-Neustadt It has been found that when the body is put under a chronic unpredictable stress it can lead to considerable neuronal damage. It does so by reducing the expression of Brain Derived Neurotropic Factor (BDNF) located in the hippocampus. This protein is known for supporting the survival of existing neurons and promoting the growth of new neurons (Altar, 1999; Lindsay et al., 1994). To combat this neurodegeneration, our study set out to determine if a neuron enhancing effect could be observed in experimental conditions using a chronic unpredictable stress model paired with physical exercise. Male rats will be split into a control group and two experimental groups. The two experimental groups will be subjected to various stressful situations (tail suspension, food/water deprivation, prolonged exposure to light, submergence in water, whole body restraint, etc). One experimental group will be allowed voluntary wheel running so that we can observe whether there is a supported growth in BDNF as a result of the exercise. Using In Situ Hybridization to analyze BDNF/mRNA levels, our results are expected to show some neuronal degeneration in the hippocampal area (C1, C2, CA3 and dendate gyrus) amongst the stress-only rat group. We also expect to see supported growth in BDNF amongst the rats that received exercise as a treatment. top Poster #16- Jeré Wilson Cloning of bacterial essential genes using a modified vector with Ligation-independent cloning (LIC) strategy Jeré Annette Wilson, Lilian J Real, H. H. Xu Abstract: Novel antibiotics are needed in order to combat increasing spread of multi-drug resistant strains of bacteria, as well as the threat of bioterrism. Recent advances in microbial genomics and functional genomics have identified sets of essential genes from a number of bacterial species. Most essential genes encode protein product, which could be potential targets of new antibiotics. Our laboratory has been able to clone essential genes from Escherichia coli to study functions of essential proteins encoded by these genes and to identify cellular targets of antibacterial compounds. Previous results in our laboratory indicated that over-expression of E. coli essential genes led to enhanced resistance of E. coli host cells toward inhibitors specifically targeting the expressed proteins. Ligation-independent cloning (LIC) is an efficient cloning approach that is used for directional cloning of PCR products without the use of conventional restriction enzyme digestions or ligation reactions. Currently, however LIC cloning kits are only available from commercial vendors and do not provide enough flexibility for controlled expression of native polypeptide encoded by cloned genes. In this project, our objective is to modify pLEX5BA into an LIC vector, pLEX5BA/LIC/kan. This poster describes progress of this. Methods: Five of E. coli essential genes have been PCR amplified and cloned into an expression vector pLEX5BA/LIC/kan, under the control of an ITPG-inducible promoter. Over-expression of cloned essential proteins was obtained from E. coli cells of these clones under appropriate inducing conditions.Results: In this study a total of five essential genes were cloned into pLEX5BA/LIC/kan (murE, nusA, yacE, fabB, secE ). Over-expression of their encoded proteins was confirmed via polyacrylmide gel electrophoresis.Conclusions: We have validated the usefulness of an LIC vector constructed in our laboratory. The pLEX5BA/LIC/kan provides an inexpensive alternative to commercial LIC cloning kits. In the future I plan to clone foreign essential genes of A. baumannii, using pLEX5BA/LIC/kan, followed by over-expressing encoded essential proteins and determine if inhibitor-specific resistance will be observed. top Poster #17- Ricardo Sanchez Cysteine Oxidation Prediction Algorithm (COPA): Predicting cysteine oxidation Susceptible Thiols from Protein Structure. Ricardo Sanchez1, Megan Riddle1, Dr. Jongwook Woo2, Dr. Jamil A. Momand1 1Chemistry and Biochemistry, 2Computer Information Systems, CSU, Los Angeles 5151 State University Drive, Los Angeles, CA, 90032 Under oxidizing conditions a sulfhydryl group can be oxidized into sulfenic acid (S-OH), sulfinic acid (SO2H), sulfonic acid (SO3H) and, most commonly, disulfide bond (S-S). Oxidizers can increase or decrease the function of target proteins by oxidizing protein sulfhydryl groups. It is believed that certain thiols on the surfaces of proteins are susceptible to oxidation because of two major factors: the solvent accessibility of the sulfur atom, and the presence of atoms that can stabilize the thiolate ion created by deprotonation of the thiol. Our goal is to utilize these factors and other physicochemical parameters to create an algorithm that predicts which protein thiol groups are susceptible to oxidation. With a program we created that takes PDB files as input, we extracted 10 physicochemical parameters for each target thiol to form a decision table. An 11th parameter was used as a label (1/0) to indicate whether the thiol moiety is susceptible to oxidation. Using a classification program (the C4.5 decision tree classifier re-implemented as the J48 classifier), a decision tree was obtained. The classifier selected three parameters critical for prediction of thiol oxidation susceptibility: (1) distance to the nearest cysteine sulfur atom, (2) solvent accessibility, and (3) pKa. The classifier was optimized to correctly predict 136 of the 161 cysteine thiols susceptible to oxidation. Leave-one-out cross validation analysis showed that the percent of correctly classified cysteines was 80.1% and that 16.1% of the oxidation-susceptible cysteine thiols were incorrectly classified. The algorithm developed from these parameters, named the Cysteine Oxidation Prediction Algorithm (COPA), is presented. COPA-mediated prediction of oxidation-susceptible sites can be used to locate protein cysteines susceptible to redox-mediated regulation and to identify possible enzyme catalytic sites with reactive cysteine thiols. top Poster #18- Cari Wilson Response of Breast Cancer Cell Proteome to Hydrogen Peroxide Caridad Wilson, Shefali Sharma, Jamil MomandDepartment of Chemistry and Biochemistry, California State University Los Angeles Hydrogen peroxide is a natural signaling molecule generated by oxidases and mitochondria during respiration and has been shown to mediate apoptosis in cells. This project is aimed towards investigating the molecular response of human breast cancer cells to hydrogen peroxide. One way to analyze the response is to determine which proteins are up- or down-regulated upon hydrogen peroxide treatment. Previous investigations used two-dimensional (2-D) gel electrophoresis to separate and analyze the proteome response to hydrogen peroxide treatment in the MCF-7 human breast cancer cell line. One protein exhibited a 3.2 fold increase in the presence of H2O2. The identity of the protein, determined by mass spectrometry, is either THO complex 3 or SLAP (Src-like adaptor protein). THO complex 3 is a more likely candidate since its molecular weight and pI most closely matched the unknown protein. It was found to be part of the TREX (transcription/elongation) complex which is involved in the transport of mRNA from the nucleus to the cytoplasm. THO complex 3 has also been suggested to be involved in protein-protein interactions and molecular evidence suggests that it is required for transcription elongation. SLAP is predicted to be involved in DNA replication and recombination, suggesting that hydrogen peroxide may trigger its overexpression in order to repair DNA damage. The goal of our project is to confirm the identity of the upregulated protein and determine if it helps the cells survive H2O2-mediated apoptosis. The identity of the protein (with respect to hydrogen peroxide response) will be elucidated by Western Blot analysis. Finally, cells will be analyzed for increased or decreased sensitivity to a knockout of the identified protein. top Poster #19- Suzi Sanchez Using a gene knockout approach to elucidate essential genes in Acinetobacter baumannii Authors: Suzi Sanchez# and H. Howard Xu The recent emergence of multi-drug resistant strains of bacterial pathogens has revived the imminent need for the development of novel antibiotics. Most currently used classes of antibiotics all target the products of essential genes. Consequently, identification and validation of essential genes from bacterial species are critical to discovery of novel antibiotics. Acinetobacter baumannii epitomizes the current trend, being documented in multiple sources as the causative agent in an array of nosocomial infections. It is believed that A. baumannii dedicates a large portion of its genome to pathogenecity and that it has acquired a considerable amount of foreign DNA through horizontal gene transfer conferring multi-drug resistance. We propose to use a recombination based gene knockout method to identify essential genes. The A. baumannii ATCC 17978 chromosome contains homologous copies of the fabB gene known to be essential in related organisms. This gene codes for the b-ketoacyl-acyl carrier protein synthase I, an essential enzyme in bacterial type II fatty acid synthesis. In order to determine whether one or both copies of the fabB genes are essential, a suicide vector construct will be used to knockout both copies of the gene, either individually or in sequence.middle region of each gene as a suicide vector to conduct knockout experiments within this strain. Poster #20- Ivy Donaldson Factors predicting child obesity in an ehtnically diverse community sample Ivy C. Donaldson and Fary Cachelin, Ph.D. Childhood obesity has become a major health concern in the United States, particularly among ethnic minority youth. Research has identified several factors that may potentially increase the risk of childhood obesity including parental feeding attitudes and practices, socioeconomic status, maternal weight status, amount of fast food consumption and television watching. Most studies to date have focused on European American and African American populations. The purpose of this study was to examine predictors of childhood obesity in a large, ethnically diverse community-based sample. Participants were 563 mothers with a child between the ages of 2 and 11 (306 Hispanic American, 76 Asian American, 36 African American, 145 European American/White). Mothers completed questionnaires assessing demographic information (maternal height, weight, age, occupation, education, ethnicity, generation status), child characteristics (gender, age, height, weight), child eating and activity patterns (times per week eat fast food, hours per week watch T.V.), and the Child Feeding Questionnaire (Birch et al., 2001) composed of the following subscales: perceived responsibility for child feeding; concern about child weight and eating; restriction of child eating; pressure on child to eat; monitoring of child eating. Multiple linear regression indicated that in this model (R2 = .14, p < .000) significant predictors of child's body mass index (BMI) were hours per week child watches T.V. (p = .02), mother's concern about child weight and eating (p = .002), pressure on child to eat (p = .01), socioeconomic status (p = .03), and ethnicity (p = .02). Specifically, higher child BMI was associated with more hours of television watching, more concern with child eating and weight, less pressure on child to eat, lower socioeconomic status, and being African American. The remaining variables (mother's BMI and generation status, times per week child eats fast food, perceived responsibility for child feeding, restriction of child eating, monitoring of child eating) were not significant predictors of child BMI. Findings point to the need to consider a number of demographic and attitudinal factors in the prevention of childhood obesity. Poster #21- Poster #17- TOP | Cal State L.A. | MORE Programs Webpage CSULA Dept. of Chemistry and Biochemistry | Biology and Microbiology | Psychology


text:
Wholesale Product Spotlight – Rustic Wishing Well Planter
Incorporating a water feature into your overall landscaping plan has become very much on trend. Although the term may be relatively new the concept is not and one of the earliest forms of a 'water feature' to be installed in backyards all over the world is the wishing well. Our Rustic Wishing Well is a perfect modern example of such a classic feature.
There was a time, long ago, that the well served a practical purpose and its aesthetics were a secondary consideration. Even after that no longer was the case, people still held on to their wells, realizing that they actually were rather attractive.
These days a wishing well like this one adds a rustic charm that evokes images of those times gone by in a way that is very charming and will enhance almost any garden decor.
Our Rustic Wishing Well Planter stands a generous 25 5/8″ diameter x 44 1/8″ high and is crafted from high quality, durable fir wood. It is finished with an attractive dark stain and its decorative bucket serves as an attractive planter, adding to the visual appeal of this lovely piece of outdoor decor even further.
Buy the Rustic Wishing Well Planter wholesale at Koehler Home Decor.

subset: commoncrawl


text:
Handbook of Research on Strategy Process
Edited by Pietro Mazzola and Franz W. Kellermanns
Chapter 5: Strategy Process Research and the RBV: Social Barriers to Imitation
Patrick Regnér
Patrick Regnér INTRODUCTION The primary mission of strategy process research has been to analyse how strategies develop. While the approach taken has varied between diverse research efforts, conclusions have confirmed that strategy making is much more complex than top management strategy design and choice, as often explicitly or implicitly assumed in strategy content research (e.g. Rumelt et al., 1994). At the most fundamental level it has been concluded that contexts matter for strategy development and change (e.g. sociocultural, cognitive, political influences). Unfortunately the consequences of this for strategy outcome and content have often not been spelled out (Chakravarty and White, 2002), and if they have been analysed it has frequently been through the lens of flawed and biased processes where various contextual influences distort strategy making. The focus has been on how social contexts and interests disturb economic interests rather than on how they can shape economic outcomes. While strategy process studies have provided much valuable contributions to strategic management research it would benefit from relating more explicitly to strategy content and outcome (Chakravarty and White, 2002). The division between strategy process and content research is artificial and process examinations may complement and inform strategy content research in many ways. This essay proposes that there are important synergies between strategy process research and resource and capabilities views, which have dominated much of strategy content research during the last decade. The basic argument follows other scholars that have pointed towards a potential fertile combination between the two research strands, as...
You are not authenticated to view the full text of this chapter or article.
Elgaronline requires a subscription or purchase to access the full text of books or journals. Please login through your library system or with your personal username and password on the homepage.
Non-subscribers can freely search the site, view abstracts/ extracts and download selected front matter and introductory chapters for personal use.
Your library may not have purchased all subject areas. If you are authenticated and think you should have access to this title, please contact your librarian.


text:
Should patient consent be considered in sharing health info across national network?
The steady migration to electronic health records, mandated by the HITECH Act, may lead to inevitable trade-offs between privacy and security on the one hand, and more efficient and, ultimately, perhaps, better health care on the other. At the heart of the matter are the Regional Health Information Organizations (RHIOs) that are critical to developing a National Health Information Network.
In a posting on the American Civil Liberties Union (ACLU) website, Corrine Carey, assistant legislative director, New York Civil Liberties Union, addresses the issue of patients' health records being uploaded into massive RHIO databases without their consent. She addresses several cases in which patient data might be accessed without their consent, but zeros in on data breaches resulting from malicious cyber attacks or sloppy data handling practices or sloppy data handling practices (lost or stolen laptops, backup media, USB drives, etc.).
"In light of the tremendous risk to privacy posed by ubiquitous security breaches, it is critical that patients have the ability to consent to making their personal health information available electronically," she writes.
I'm not sure that's on the mark.
The security risks are very real. She is quite correct on that point. We have born witness to a rash of health information breaches in recent months, and there is considerable evidence that the healthcare industry lags behind other sectors in data protection. So we have the perfect storm:
- A rapid increase in highly sensitive personal data being transmitted and stored digitally and proliferating across distributed, electronically linked repositories
- Inadequate data protection
- Aggressive cyber criminal attacks
But here's the rub. We hear justifiable complaints about our healthcare systems' inefficiencies. Storing, transmitting and sharing patient medical information electronically, in standard formats, makes all the sense in the world. The upfront costs of conversion are high (under HITECH, the government has taken a carrot-and-stick approach, with incentive payments to organizations that comply in a reasonable time frame, and penalties for those that do not), but the potential advantages are clear.
So is patient consent to the distributed sharing of medical information really a debatable issue? We are not talking about sharing records with tabloids. We are talking about a coordinated healthcare information exchange network. If the goal is lower costs and improve healthcare, the discussion should not focus on patient consent, but on better data protection. By its very nature electronic data is more susceptible to loss and theft. If we stipulate that sharing it across this proposed national network is ultimately a good thing, then it is incumbent upon the entities that are custodians of the data to do a better job of securing it.
The recent breaches have had nothing to do with sharing health records and everything to do with poor data protection policies and procedures. True, the more entities that have sensitive data, the higher the risk of theft or loss. So talk about in the same terms that security is considered in the business world. What is the risk vs the benefits? I come down on the side of the benefits on this one.


text:
From July 2002 through Fall 2010, The Judy Room featured "magazine covers" as the homepage art. These covers were meant to fondly evoke the heyday of the movie fan magazines of Hollywood's Golden Age, while also functioning as a modern website homepage.
Like a magazine of old, these covers changed with each season (Fall, Winter, etc) and sometimes featured "special issues" for holidays.
All of the design and artwork for these covers were created by me, unless otherwise noted.
Clicking on the thumbnails below opens pop-up windows featuring larger images of the covers. Click here to head back to the Judy Room Archives page.


text:
The FAIR TRADE Mark is an independent consumer label which appears on products as an independent guarantee that disadvantaged producers in the developing world are getting a better deal. There are now over 1,500 Fairtrade retail and catering products available in the UK.
Fairtrade is about better prices, decent working conditions, local sustainability, and fair terms of trade for farmers and workers in the developing world. St John's became a Fairtrade Church in 2004 and joined almost 7,000 churches who support this campaign. Bradford Diocese has been awarded Fairtrade status as over 50% of its churches are actively committed to Fairtrade.
| Printable Version


text:
Comfort Zone
If you are reading this then you are ready to make bold decisions and take bold steps. Don't worry, I will not trick you into shaving your head...unless you really want to.
Getting Out
One of the most courageous things you can do is to get out of the comfort zone; the warm, peaceful place that you know the boundaries, the reasons and the consequences.
It doesn't matter if it's good or bad. You KNOW what will happen and it gives you a safe feeling.
To Grow or Not To Grow
Although the comfort zone is a beautiful place, the most important feature is that nothing, including you, can grow in it. If you want to develop as a person, as a good friend, as a partner, as a parent...then the comfort zone is your biggest enemy! Get out of that comfort zone immediately.
How To Walk Away
Let me give you a few small tips to walk you out towards the exit of your comfort zone. Let's try these small steps during the week. You will enjoy it i promise.
1- Wear your watch on your opposite hand.
2- Find an event outside your usual interests and try it.
3- Listen to a song in a language that you don't know
4- Pick a century and learn something interesting about it.
5- Write down your guilty pleasures
6- Write down your biggest mistake and burn that piece of paper. (ıt is not defining you anymore)
7- Go and explore a new neighbourhood.
Weekly Events – Join In!
View our current events by clicking here
Leave a Reply.
April 2018


text:
Manchester City left back Benjamin Mendy has joked about joining a new club on loan after news emerged of his injury on Thursday evening.
Having joined the Citizens from Ligue 1 champions Monaco in the summer, Mendy has made a huge impression in England both on and off the pitch. The Twitter hero has rose in popularity amongst British footballing fans for his social media antics - making jokes about his FIFA 18 rating and ripping into teammate Kyle Walker.
But now, after hearing that he has ruptured his anterior cruciate ligament, he's maintained his playful online persona and made fun of his strife.
The French left back came off in the first half of City's 5-0 thrashing of Crystal Palace last weekend, and after initial scans left Pep Guardiola feeling optimistic over the scare (the Spaniard claimed that Mendy would only be out for a few days), the defender's season could well be over already.
Mendy travelled to Barcelona this week to see a specialist regarding his injury, and will now go under the knife on Friday - facing months on the sidelines.
The news poses huge problems for Guardiola, who is now without a dedicated left back. City's midweek match against Shakhtar Donetsk saw Fabian Delph fill in Mendy's position, and the only other suitable option ahead of the club's weekend encounter with Chelsea comes in the form of natural right back Danilo.
According to the Mirror, City will now delve into the January transfer market to find a left back in Mendy's absence - with fears growing that his recovery could keep him out until the end of the season.


text:
Boulder Uber Accident Attorneys
Hurt in an Uber Crash? Call Now
Since Uber's arrival in Colorado, thousands of riders have enjoyed using the convenient and affordable ridesharing company to take them to the places they want and need to visit in Boulder. Yet, like other vehicles on the road, Uber riders and other drivers are at risk for being injured in an accident. In some cases, the accident might be due to the negligence of the Uber driver, or it might be the fault of another driver. In either case, if you were injured in an accident involving an Uber driver, you might be entitled to seek compensation that is directly related to your injuries. Call the skilled Boulder car accident attorneys at the Tenge Law Firm, LLC at (303) 665-2929 to discuss the details of your case and determine your eligibility to recover damages.
The Tenge Law Firm, LLC's Past Results
The legal team at the Tenge Law Firm, LLC has extensive experience handling many motor vehicle accident cases, including those that involve an Uber driver. In recent cases, the firm recovered more than $800,000 for a woman who was in a rear-end collision, $950,000 for a woman who was hit by a teen driver while traveling, and $1,350,000 for a driver who was in a head-on collision on her way to work in Denver. These examples of past results do not guarantee a particular outcome in your case, but the Uber accident lawyers at the Tenge Law Firm, LLC in Boulder will fight to get the best possible outcome for your individual situation.
Causes of Uber Accidents
Many scenarios might lead to an Uber accident in Boulder. Although each accident is different, here are some common causes of Uber accidents:
- Distracted driving - Driving while distracted has long been a cause of motor vehicle accidents, but Uber drivers are especially vulnerable. In addition to normal distractions like food, radios, and talking or texting on cell phones, Uber drivers might be distracted by loud, drunk, or annoying passengers or using a GPS to find their next pick-up or drop-off location.
- Drowsy driving - Uber restricts drivers to 12 hours per day, but that doesn't completely eliminate the problem. While the 12-hour limit includes driving, it does not account for long breaks between rides or other employment.
- Drinking and driving - Alcohol and other controlled substances result in the same risk that it does for other motor vehicle drivers.
- Speeding - Violating traffic regulations might lead to accidents, but Uber drivers are most likely to speed. Riders want to get to their destinations quickly, and Uber drivers want to give as many rides as possible to make more money. Speeding doesn't always result in a crash, but it does increase the likelihood of severe injuries and fatalities when crashes do occur.
- Tailgating - Following another vehicle too closely is another artifact of Uber drivers being in a hurry. Tailgating doesn't allow enough distance for an Uber driver to respond if the car in front of them stops or turns quickly.
- Poor maintenance - If an Uber driver doesn't maintain important systems in their vehicle, accidents might occur. Examples include worn tires and brakes.
Seeking Compensation After an Uber Accident in Boulder
If you, another driver, or an Uber rider was injured because of an Uber driver's negligence, you might be entitled to compensation for losses related to your injury. Uber accidents, like most auto accidents, might result in complex legal cases with multiple parties, especially with more than one vehicle and/or passenger involved. Colorado is a tort liability state, so you will need to file a claim with the at-fault driver's insurance company. Colorado's Senate Bill 25, the Transportation Network Company Act, requires Uber to provide primary coverage for liability when the driver is signed into the Uber App. This means you may have to deal with Uber's insurance carrier, which is Progressive Insurance in Colorado.
Depending on the severity of your injuries, filing an insurance claim might be enough to get compensation for your damages; however, insurance companies do not like to pay out claims. With the complexity of Uber accident cases, it is in your best interest to hire a lawyer to advise you if and when the time is right to escalate an insurance claim to a personal injury lawsuit. In the event that you sue, you might be able to recover the following damages:
- Medical costs including emergency services, hospitalization, x-rays, surgery, doctor visits, medication, and more
- Lost wages for time away from work due to your injury
- Future medical costs in the event of a long-term disability or catastrophic injury that requires a long time to fully recover
- Future lost wages if an injury results in a disability that prevents a victim from returning to their job
- Non-economic damages for pain and suffering, loss of consortium, scarring, disfigurement, and more
Contact a Skilled Uber Accident Lawyer in Boulder
A skilled personal injury attorney who has experience with Uber accidents will anticipate counterclaims and other strategies that the defense might use to avoid paying damages. Additionally, they can help with investigating your Uber accident, gathering evidence and relevant documentation such as medical reports, police reports, and witness statements, enlisting the help of experts to speak to support your case, and negotiating a full and fair settlement with insurance companies. When insurance companies are not willing to offer full and fair compensation, we are prepared to litigate your case and diligently pursue the compensation that you deserve.
Contact the skilled legal team at the Tenge Law Firm, LLC online, or call our office in Boulder at (303) 665-2929 for a free consultation to discuss the circumstances of your Uber accident before Colorado's statute of limitations runs out. You have three years to take legal action in Colorado when a motor vehicle is involved. We are empathetic to the struggles that come with a serious injury and wish to help you through this difficult time, so you can focus on recovery and rehabilitation. If you choose the Tenge Law Firm, LLC to represent you, we handle personal injury cases on contingency. This means you won't pay attorney fees unless we secure a settlement or verdict in your favor.
Our team is here to help. Call us or submit our contact form to schedule your free, no-obligation case evaluation.
(303) 665-2929
"I could tell he had decades of experience in cases like mine, which gave me a lot of comfort during such a stressful time."
Reviewed by Jennifer on Avvo
"I placed all my trust in them and was not disappointed! They settled my case for an amount I had never expected even in my wildest dreams."
Reviewed by Georgiana on Google
"We did not have to pay any bills or answer any calls from doctors, hospitals, or insurance companies. We were well taken care of."
Personal Injury Client on Lawyers.com


text:
New video on YouTube check it out!
Norway Maple (Acer platanoides) - tree - September 2017
A Norway Maple within the Hill Barton industrial park of Exeter, UK. ==== Norway maple is a deciduous broadleaf tree native to northern Europe and was introduced to the UK in the 17th century. Norway maple was introduced to North America in the early 20th century and is now considered an invasive species. Because it is so tolerant to shade, it out-competes the North American native sugar maple. However, its canopy is denser than that of sugar maple, so fewer wildflowers are able to grow beneath, reducing biodiversity. Mature Norway maple trees can grow to 25m. The bark is grey with fine ridges, and the twigs are slender and brown with tiny white spots. The leaves are palmate and have five lobes with a few pointed teeth. They are dark green in colour, fading to yellow and occasionally red before falling in autumn. All Norway maple flowers grow in clusters of up to 30. Once pollinated by insects, female flowers develop into winged seeds, or samaras, which fall in autumn and are distributed by wind. Native to eastern and central Europe, from France east to Russia, Norway maple can be found in the UK as a street tree and is widely planted as an ornamental tree in parks and gardens due to its tall trunk and tolerance of compacted soils, shade and pollution. Norway maple timber is similar to that of sycamore, being hard, strong and pale cream in colour. It may be used for a variety of situations, including furniture and turnery. However it is not often grown commercially due to problems associated with grey squirrels, which strip the bark. Trees are also planted widely in towns and cities, thanks to their ornamental value, and tolerance of shade and pollution. The leaf of the maple tree is incorporated in the flag of Canada. Maple tree can survive more than 300 years under appropriate climate conditions. People in Japan like to watch delicate changes in the colour of the foliage during the autumn. Collective watching of maples in the autumn is known as "momijigari" in Japan. Maple tree is also used in the paper industry. Paper made of maple tree has excellent printing properties. Dried wood of maple tree can be used for smoking of food, while charcoal made of maple tree plays significant role in the manufacture of Tennessee Whiskey. === Scientific classification Kingdom: Plantae (unranked): Angiosperms (unranked): Eudicots (unranked): Rosids Order: Sapindales Family: Sapindaceae[1] Genus: Acer Species: A. platanoides Binomial name Acer platanoides Category Education Licence Standard YouTube Licence Category Education Licence Standard YouTube Licence
Leave a Reply.
Write something about yourself. No need to be fancy, just an overview.
August 2019


text:
Camille Cristina Reyes and Theresa Soriano
Peter F. Drucker: The Godfather of Modern Management
Theres nothing permanent in this world. Everything changes fast. Time can change things drastically. In Peter Druckers book, Managing in Time of Great Change, he emphasized on the changing environment that we live in. The book talked about the management, information-based organization, economy, and society. The book encapsulates the emergence of information as executives key resource and as the organizations skeleton.
Drucker s Theory of Business refers to the assumptions that shape any organizations behaviour, dictate its decisions about what to do and what not to do, and define what the organization considers meaningful results. They are about marketcustomers, competitors, their values and behaviours together with a companys strengths and weaknesses. These assumptions are what a company gets paid for. Also, a valid theory of business has an environment, mission and core competencies that fit reality and fitted to one another. The theory of business must be known and understood throughout the organization and it has to be tested constantly.
According to Drucker, uncertainty can become an opportunity. With strength analysis and matching of companys strengths to the changes that have been made, unexpected things in a business will turn to an advantage. Dont be afraid to respond to every opportunity encountered.
One of the highlights of this book is 5 deadly sins of business. These must be avoided in order to protect a business.
1. The worship of high profit margins and premium pricing
-This opens the doors for competitors.
2. Mispricing a new product by charging what the market will bear
3. Cost-driven pricing
-The only thing that works is price driving.
4. Slaughtering tomorrows opportunity on the altar of yesterday.
5. Feeding problems and starving the opportunities.
Only opportunities produce results and growth.
Theres no excuse for managers to indulge in the five deadly sins. They are temptations that must be resisted. One solution is to start with what the market is willing to pay.
Family serves the business, not business is run to serve the family. Business cannot be mixed with family. As a solution to problems like when there is an incompetent family member in the organization/ company, is to entrust the succession decision to an outsider who is neither part of the family nor the business. Hire a competent non-family member employee rather than saving cost for a lazy family member employee. This is true and we suppose is one of the values that gave the Chinese people a success in their economic status today.
Drucker also mentioned about the six rules of presidents. A president is committed to the people and the law so he must not stubbornly do what he wants to do. He must have a mindset on the needs of the people. A leader like a president should not micromanage thus making sure that operations are done appropriately. He also shouldnt have a friend in the organization that may tend to abuse their position and power. Power as a president should be used responsibly.
In his book, he also talked about the economys power shift. He reiterated that what underlies the shift is information. Analyzing information from a simple action done by the customer to a data that will help the company improve their service. Through the real-time information handled by a company or an organization, decision-making power of the people will also be tested. Another implication of the power shift is that companies today tend to organize themselves internally around the flow of information; we now call it today as re-engineering. Companies today found that they did not need many good management levels. The biggest implication is that, according to Drucker, the economy is changing structure. This change is from being an organized company around the flow of things and the flow of money; it is becoming organized around the flow of information.
Since, changing time is the main focus of the book, it also described how new markets are. Economic growth and expansion today is not focused on the consumer demands anymore, rather they seek for accessible new markets such as communication and information, environmental market, need of repair, replenish and upgrade physical infrastructure and investment to finance survival into old age. Marketing relationships should be taken care of, customer rather than own product.
In time when there is a need to change, the author believes every manager has to build the management of change into its very structure. Every organization has to prepare for the abandonment of everything it does. Every organization should plan abandonment rather than trying to prolong the life of a successful product. There should be self improvement. Another thing is that, they should learn how to exploit their knowledge to develop the next generation, and they should learn how to innovate. The author mentioned that Knowledge is the primary source of individuals and for the economy overall. A specialized knowledge can only become productive if it will be integrated to a task. Only a focused and common mission will hold the organization together and enable it to produce. Without a mission, credibility will be lost. The organizations most essential resource are the qualified knowledge people and the greatest asset are people.
Each organization has a value system that is determined by task. He explains that for the organization to perform to a high standard, its members must believe that what it is doing is in the contribution to community and society on which all others depend. Therefore, members must believe on what they are doing in order to have a high standard performance.
Work for the organization, not for the boss. Business growth will be attained if there is ownership meaning a company must know their strengths so that they can market themselves, and partnership, which entails working with people.
Goods, no matter how greedily desired, change consumption; information changes imagination. Goods change how we live; information how we dream. Goods change how we see the world; information changes how we see ourselves. Change should be seen as a good choice in making a difference.
Guide questions:
1. If you will be a manager, how will you handle your team? What characteristics of a leader that you will see to it that you possess?
2. As a leader, how will you deal with our changing environment?
3. Considering that you are a nurse, supposed that you will be inheriting a big family business, will you accept it? How will you run it?


text:
Ask any personal trainer or fitness professional what they would buy if they were only allowed to purchase one piece of strength training equipment and the Flat/Incline/Decline Bench is sure to be at the top of the list.
Specifically designed with a shortened base frame to fit perfectly in all Smith Machines, Power Racks and Multi-Press Racks. Allows proper form and positioning for incline press and shoulder press exercises in all racks. Improved design features quick, easy, solid and secure 6-position ladder-style back pad adjustment.
Eight-position seat pad adjustment allows all size users to do bent knee sit-ups and ab crunches with proper lower back support and hip flexor alignment. Includes T-bar Leg Hold Down Attachment and thick, rich DuraFirm™ Upholstery. Includes wheels for easy mobility. Over 600 lb. capacity.
Warranty Agreement
This Warranty applies only in the United States to products manufactured or distributed by Body-Solid, Inc.(Body-Solid) under the Body-Solid brand name. The warranty period to the original purchaser is:
BODY-SOLID warrants the In-Home Warranty as follows:
LIFETIME: Frame & WeldsLIFETIME: Pulleys, Bushings, Bearings, Hardware, Plates, Guide RodsLIFETIME: Cables, Upholstery, Grips (Normal Wear)


text:
|The Fairless
Skagway is a one-horse town: 1 library, 1 bank, 1 post office, 1 gas station, 1 grocery store--the Fairway. We call it the Fairless. A gallon of milk costs more than a gallon of gas. $5.50 compared to $4.98. Everything is shipped up once a week by barge--arriving Tuesday afternoons out of Seattle. By the time it gets here the produce is either too ripe or not ripe. Safe to say, by Wednesday afternoon it's all gone. Shelves are as empty as Old Mother Hubbard's. So our company, HollandAmerica-Princess/Alaska, combined a day of training with a shopping spree in Whitehorse, Yukon, Canada, 110 miles due north of Skagway. Hip hip harrah! I love the company I work for!
|Skagway River, early season: Bus Yard to the left
We began at the MP2 lot--the bus yard. MP2 stands for Milepost 2 of the Klondike Highway; in other words, it's 2 miles from Skagway's Port of Call. So it's at MP2 where the buses are parked and the bus shop is--with a crew of full-time mechanics who keep us up and running and legal--and the MP2 House where several of the Driver Guides live in a large dormitory. I did not choose to live there, despite the fact that it's right across the highway from Jewell Gardens where I worked last year and where some of my friends live, because it's where Blake lives--the boy, I figure, needs some space from his mother.
|MP2 Bus Yard and Housing
So we began our day of training and shopping bright and early, the agenda being to learn how to gear down for the steep windy road ahead, where to pull off for picture stops, and to visit various venues we'll be taking our tourists to. Final destination? Whitehorse, Yukon, Canada, and the Canadaian Superstore! We were excited. Food!
|Stanley and Me at picture stop just past the
Captain William Moore bridge.
|William Moore Bridge, crossing an intersection
of about 5 fault lines, 1 of 3 or 4 cantilevers in the world.
A common tour is the train up/bus down (or bus up/train down) to Fraser, B.C., Canada. The White Pass & Yukon RR is Skagway's biggest tourist draw and largest employer, and we subcontract with them to get their passengers thither and yon. I personally love the train--one of 136 civil engineering feats in the world, right up there with the Eiffel Tower, Panama Canal, and Statue of Liberty.
|View from the train, overlooking Skagway River
As a HAPA employee, I will frequently drive up the pass to either pick up or drop off tourists from or for the train--at Fraser, B.C., specifically Canadian Customs. So we practiced here on how to go through customs, what to do if we "deadheaded" up--meaning going up empty to load passengers just off the train. It was at this point we were told that if we rolled into the train station with people aboard and did not report to customs, we'd be fined $10,000 and looking at possible jail time. The fine comes right out of our own pocket, thank you, and the day we do this in all likelihood will be our last with HAPA.
|Train Tracks at Fraser, BC: Lake Bernard still iced over.
The border itself, however, is several miles back up the summit; customs is stationed here because the weather is less gruesome, "less" being the operative word.
|Fraser, BC, Housing
One of our Driver Guides is dating a Canadian Customs officer. I asked her what she'd done so wrong as to be banished to this Siberian outpost. She's just paying her dues, she says, and hopes soon to transfer back to Ontario. Who can blame her? And wish her luck? Existence up in Fraser has to be pretty rough in the winter. And pretty darn boring.
|Lake Bernard Picture Stop, just past Fraser
Just past Customs at Fraser is a scenic pullout and picture stop. It's also where we can stall for time if we're too early for the train. Most importantly, it's a mile marker in history because this is where the White Pass Trail veers to Log Cabin, a tent city that once surrounded the Mounties' 1897 Log Cabin. At Log Cabin, duty was paid before going into Canada. And where the Mounties made sure everyone had 2,000 pounds of goods. Hunger in the Yukon was a problem; this was one way the Canadian government sought to forestall mass starvation.
|Log Cabin
Just past the turn-out are railroad tracks, the train route stretching farther north, through Log Cabin and on to Lake Bennett. Crossing those tracks, the visible cabin, however, is not Log Cabin. It's foundations lie somewhere in the woods nearby. The cabin we "pretend" is Log Cabin is actually a RR maintenance shed.
|"Log Cabin Now"
We crossed the tracks and arrived into Whitehorse about 2 pm...where we were taken directly to Canada's Super Store.
|I heard someone once describe Whitehorse as a
strip mall. That about covers it.
We were given an hour to buy everything our purse and cart could support. Everyone dispersed like flies frightened by a swat and we swarmed through the aisles grabbing frozen waffles, pencils, canned stew, raw honey, milk, granola bars, peanut butter.
|After the Fairway, I mean Fairless,
the variety at the Super Store was mind-boggling.
If you spent more than $125 you got a free watermelon. I got a free watermelon.
Coming home, we were taken the back route to Miles Canyon, a once formidable spot on the Yukon River where white water rapids gave name to the city, it's frothy water looking like the mane of a spirited white horse--hence Whitehorse.
The formidable waterfalls also splintered the home made boats of 1898, built by miners at Bennett Lake. To get to the gold, miners had to catch a steamer out of Seattle for Skagway, hike either the Dead Horse or Chilkoot Trail, then build a boat on Lake Bennett where the trails converged--and then paddle 640 miles downriver to the gold fields. In 1898, Miles Canyon was turning the Yukon River into the River Stix for the majority, and so the Mounties again stepped in by requiring building codes that eliminated fragile boat construction.
The formidable waterfalls also splintered the home made boats of 1898, built by miners at Bennett Lake. To get to the gold, miners had to catch a steamer out of Seattle for Skagway, hike either the Dead Horse or Chilkoot Trail, then build a boat on Lake Bennett where the trails converged--and then paddle 640 miles downriver to the gold fields. In 1898, Miles Canyon was turning the Yukon River into the River Stix for the majority, and so the Mounties again stepped in by requiring building codes that eliminated fragile boat construction.
|Miles Canyom 1898
|Miles Canyon 2011
But just in case anyone asks why I happen to like working for HAPA? What other company in the world takes you for a drive through paradise and calls it training? And gives you an hour off to go shopping? I mean, really?


text: This position paper presents a small set of organizational design patterns formulated within a long-term R&D program on how to enhance the quality of the digital work environment of Swedish industry and public agencies. It then relates the patterns to two of the "next steps" that the Silberman et al. article, referred to in the CFP, concludes with. This amounts to an operationalization of possible next steps for HCI sustainability research aiming for enhancing the social sustainability of business critical workplace systems. The paper argues that these steps would not only benefit the long term social sustainability of tomorrow's working life. They may also be regarded as facilitators for harnessing the innovative potential of employees, consumers and citizenry that is needed in the slow, ongoing transformation to a more sustainable society.


text:
Despite relief from SC, Aadhaar still mandatory for income-tax returns
It has been 10 days since the SC indefinitely extended the deadline for linking Aadhaar to various services, but the Income Tax (I-T) website continues to grossly violate laws.
Even now, the website is barring people from filing I-T returns without quoting Aadhaar or enrolment number.
However, exemption continues for very senior citizens (80 years+), NRIs and those living in Assam, J&K and Meghalaya.
SC extended Aadhaar-linking deadline indefinitely till it pronounces judgement
On March 13, while hearing petitions against Aadhaar, the SC indefinitely pushed back the deadline for linking it to various schemes till it pronounces its verdict in the case.
Previously, the last date stood at March 31.
Currently, no agency, including banks and telecom service providers, is authorized to act against subscribers who don't have Aadhaar linked.
But it'll be necessary for social schemes.
In accordance, telecom department, insurance regulator extend deadlines
Since then, the telecom department has issued orders extending the deadline for mobile number re-verification through Aadhaar, till the SC pronounces its order.
The Insurance Regulator and Development Authority of India (IRDAI) also issued similar guidelines for existing policy-holders.
New subscribers have been urged to submit Aadhaar, but if they don't have it, they can use other "official valid document" like passport or Voter ID.
However, I-T, banks and schools continue to violate orders
However, some like I-T are still demanding Aadhaar. Quoting Aadhaar for filing I-T returns is mandatory under the Finance Act, 2017 for which it has to be linked to PAN.
Mumbaikars have complained about banks and telecom providers violating laws similarly.
"We brought admission forms from various schools and all had compulsorily asked for the child's Aadhaar details," said Preeti B, a Mumbai resident.
Meanwhile, SC asks Center to justify demanding Aadhaar from pensioners
Responding to the issue, a CBDT spokesperson said, "We are (aware) of this matter and are looking into it."
Meanwhile, in the SC, the court asked the Center to justify mandatory Aadhaar for drawing pension.
It pointed out that pensioners are retired government employees, and their identity isn't under doubt.
Forcing 70-80-year-old people to get Aadhaar would fall under "exclusion," it said.


text:
Dave Bragg
Managing Director, Strategic Research
Dave Bragg
Managing Director, Strategic Research
Dave is Managing Director at Green Street Advisors, the preeminent provider of real estate and REIT research in North America and Europe. He leads the firm's strategic research, which focuses on topics across property sectors in the public and private markets. The goal of his thought leadership work is to inform property investors' decision-making process. Strategic research encompasses topics including public-private market valuation, market selection, demographics, policy, and technological disruptors. Additional areas of focus specific to REITs include REIT capital allocation, balance sheet management, and corporate governance. Previously, Dave led the firm's residential research team, covering the apartment, single-family rental, student housing, manufactured housing, and self-storage sectors. Dave is a frequent speaker at industry conferences and has been quoted widely in the financial media, including The Wall Street Journal, The New York Times, Bloomberg, Grant's Interest Rate Observer and Barron's. He is a member of the Urban Land Institute (ULI) - serving as Chair of the Technology and Real Estate Council. Dave is also a member of the National Association of Real Estate Investment Trusts (Nareit), the Pension Real Estate Association (PREA), the National Multifamily Housing Council (NMHC), and the Association of Foreign Investors in Real Estate (AFIRE). Prior to joining Green Street in 2013, Dave developed and led the REIT research effort at Zelman & Associates. Before that, he was a member of the REIT teams at ISI Group and Merrill Lynch. Dave earned an M.A. in International Business from the University of Florida and a B.A. in International Relations from Tufts University.
Our Thought Leaders
With over 100 employees, including more than 50 research professionals, Green Street provides actionable intelligence that keeps commercial real estate investors on the leading edge of where markets are headed. Our dedicated team covers 128 listed real estate companies, and property sector experts focus on a maximum of 10 to 12 companies. We bridge the gap between public capital markets and the private real estate market through our award-winning research and proprietary data sets, customized advisory services and experts in Europe.


text:
Many clients ask us, where to go for dining in Budapest with friends or with a gorgeous company of a lady, which restaurants should they visit. Since the growth in gastronomical selections in the past few years in Budapest. We would like to share a little selection depending on the prices and needs. And to make sure that they experience the best.
Top restaurants with affordable pricing in Budapest
The "affordable" expression is depending on our financial limits, but we can recommend these restaurants, to whom are interested in what is the best in Budapest.
Borkonyha (Bp, 5th district Sas u. 3.)
One of the Michelin star restaurants in Budapest, which we could describe as a fusion of a french bistro and a Hungarian contemporary family restaurant. A "Borkonyha" (Wine kitchen) name suggests that they have a great selection of wine, approximately 200 different kind of wines, mostly Hungarian. With fresh, daily acquisition, and with weekly or 2 weekly changing food selection. The best choice for a gourmet.
Tanti (Bp, 12th district, Apor Vilmos square 11-12, in the Hegyvidék shopping centre)
A Michelin star restaurant. The Tanti restaurant is located on a pleasant place at the Buda side in the mountains, far away from the downtown's whirl. Since their opening in 2014 they are gathering popularity, just after 6 months they got the Michelin star acknowledgement. The service is really nice, very homelike. They want to avoid snobbery. The food is truly a masterpiece. The seafood lovers can find extraordinary dishes on the menu.
Top restaurants in Budapest if we don't have any financial limits
These restaurants can be useful, if we are taking business partners on a dinner and we have a business credit/debit card and we sign the disbursement :)
Fuji (Bp, 2nd district, Csatárka út 54.)
If we are thinking about the asian cuisine, then the Japanese restaurant Fuji would be the best decision. The restaurant got numerous acknowledgement for instance suggestion from Gault and Millau and the title of Hungary's best Asian restaurant. The authentic Japanese inside and outside style and the special natural ingredients, which they are getting freshly every single day are being taken care of by the Japanese chef itself Masato Usuda.
Nobu (Bp, 5th district, Erzsébet tér 7-8. in the building of the Kempinski Hotel)
A Japanese gastronomic speciality also. The fans of master chef Nobuyuki Matsuhisa could first try his specialities in Mid-Europe in Budapest. There is a sushi bar and a maximum 20 person capability private room in the restaurant. They are waiting their guests with Nobu cocktail specialities and drink menu in the bar.
Fausto's (Bp, 7th district, Dohány utca 3.)
In the heart of the downtown,during lunchtime and dinnertime they serve in the Ristorante the classical Italian cuisine, in a sophisticated, contemporary style. With excellent ingredients, in an elegant environment, and with mindful service. For the lovers of the Italian cuisine.
Lou Lou (Bp, 6th district, Székely M. utca 2.)
For the lovers of the French cuisine,the best decision the elegant and authentic Lou Lou Restaurant. One of Hungary's most acknowledged top gastronomic restaurants. A hidden treasure in the heart of Budapest, on which it has a great influence the haute cuisine. Imaginative flavour combinations, top quality ingredients and sophisticated presentations determine their gastronomical belief.
Baraka (Bp, 5th district, Dorottya utca 6.)
You can call this restaurant a legendary one, because for many years it has a top quality kitchen. You can not leave out mentioning the location of this restaurant, it takes place in the beautiful Dorothy Palace, in the heart of the historical downtown. One of the best fine dining restaurant in Mid Europe… Very fine dining, very fusion and very expensive. :)
Brilliant restaurants in Budapest for a date, where the dinner is not only a scenery
If you would like to taste the best of the best gastronomy in Budapest, but you don't want to be ill at ease, on the other hand spend an off-handed experienceful night, then we are suggesting the places listed below.
Laci!konyha! (Bp, 13th distrcit, Hegedus Gy. utca 56.)
For many years they have one of the most exciting restaurants in the capital city. There is no such thing as a fixed menu, but they have so much ideas in a week, which would be enough for months at other places. Hungarian tradition based sophisticated contemporary boutique restaurant. Selected offers, high quality! Just take it easy! Rating of Gault & Millau is 14 points.
Fricska (Bp, 7th district, Dob utca 56-58)
From their starting momentum they didn't lose a single bit. It's name Fricska (Flick) indicates the culinary effrontery, which they give to the well known Hungarian cuisine. We can stumble over unique Italian and French flavours, and they remodel the traditional Hungarian cuisine. They surprise the guests every single day with a new menu. Visit them in jeans or in suits but you can not miss out on their offers.
Situ (Bp, 7th district, Kazinczy utca 32.)
In the upcoming event of a friendly dinner or a date you wish to try something new, then you can rely on this restaurant. We shouldn't be taken aback by the fact that it is located in the heart of the party district! The Situ mostly concentrates on the French cuisine, but there are a few Hungarian dishes hidden in the menu (for example the unmissable Goulash soup). Quality dishes, friendly environment and with a friendly smile which even comes home with you. Drop in to the Situ!
After listing all these excellent and exciting restaurants, I can only wish for You to enjoy yourself in Budapest relishing the gastronomy also and we hope that our gorgeous escorts can make the flavours taste even better and make your experience even fuller.


text:
How to Download, Fill Out and Send In the PSO Registration Form
Now that all your security officer training is complete and your fingerprints have been taken, you're ready to send in a PSO registration form to the BSIS following the steps below.
Step 1. Get the PSO Registration Form
Get a copy of the Proprietary Private Security Officer registration application form from the Bureau of Security and Investigative Services. Click the Proprietary Private Security Officer Registration Form link under the License Application as shown in the image of the website below.
Step 2. Complete the PSO Registration Form
An image of the PSO registration form is shown below. As you can see, the form includes instructions for its completion. Below this image is important information about how to complete this PSO form.
Notes About Completing the PSO Application Truthfully
- Answer all questions truthfully. Answers that aren't true may cause your private security officer registration to be denied or revoked later. Remember you will go through a background check by the DOJ and FBI.
- Don't skip any information. Any missing information may cause your private security office registration to be denied or revoked later.
- Your registration may be denied if you have been convicted of any crime or act substantially related to the qualifications, functions or duties of a Proprietary Private Security Officer, except those crimes excluded by the provisions of Penal Code section 19.8. However, when appropriate, the Bureau will consider all submitted evidence of rehabilitation. OK, we read that Section 19.8 of the Penal Code and because we're not lawyers, we couldn't make much sense out of what it says. However, it seems that minor violations, such as some traffic infractions, public fighting, taking too many copies of free newspapers, gambling, trespassing, entering a public animal enclosure (like at a zoo or circus) and putting a body piercing in someone under the age of 18, won't disqualify form being a PSO. This isn't a legal opinion as we aren't lawyers, but gives you an idea that it's very minor convictions that could be excused by the BSIS when reviewing your PSO application. However, you could save yourself some time and money by calling and asking the BSIS if your previous criminal conviction would prevent you from getting registered as a PSO. Remember, Live Scan and PSO application fees are non-refundable.
- You must include your Social Security number. If you don't include your Social Security number, you may be reported to the California Franchise Tax Board, which may assess a $100 penalty against you. This is because your Social Security number may be used for tax enforcement purposes and for purposes of compliance with any judgement or order for family support in accordance with Section 17520 of the Family Code.
- The information you provides is used to determine your eligibility for a PSO license.
- You have the right to review the records maintained on you by the BSIS unless the records are exempt from disclosure by section 1798.40 of the Civil Code.
- Your completed application becomes the property of the BSIS and will be used by authorized personnel to determine your eligibility for a license, registration or permit.
- Information on your application may be transferred to other governmental or law enforcement agencies as permitted by law.
- The California Public Records Act (Gov. Code § 6250 et seq.) and the Information Practices Act (Civ. Code § 1798 et seq.) states that your name and address maybe disclosed if receive a PSO registration.
- Upon issuance of a registration, you must notify the BSIS of any change of address within 30 days of the change (Business and Professions Code section 136).
- And remember you can't work as PSO until you have a hard copy of your PSO Card and a government form of ID with your picture on it. You must carry these with you when on duty.
- The following California Code Business and Professions Code Chapter 3. Suspension And Revocation Of Licenses describes cases where your PSO license can be revoked.
Step 3. Write a Check or Get a Money Order for the PSO Application Fee
The application fee to become a PSO is $50.00. The application fee covers the cost of the people working at the BSIS to process your paperwork, verify that you cleared your background check, input your information into the California PSO database, print your BSIS card, mail you your BSIS and other costs associated with overseeing PSOs.
Make your check out to the BSIS.
Step 4. Submit Application Package to the BSIS
Mail the following in one package to the BSIS:
- The completed Proprietary Private Security Officer registration application.
- A copy of the Live Scan Form (BCII 8016) received from the Live Scan site, signed by the Live Scan Operator, including their ATI number.
- You application fee of $50.00 as a check or money order.
What should You Read Next?
- If you want to know more about the general steps to registering as a PSO, read How to Become a PSO.
- If you want to find out how to get fingerprinted at a Live Scan location or how to fill out the Live Scan form, read How to Get Fingerprinted / Live Scan.
- If you want to verify that your security officer Registration is up to date, read How to Verify You're Registered as a PSO.
- If you need to change some information on your PSO registration or renew your license, read How to Update / Renew Your PSO Registration.
- If you want to know more about the private security officer training requirements, read PSO Training.


text:
Welcome to the glittering Regency world of splendid balls, bespoke gowns and spring garden parties. Cottman takes readers into the heart of Georgette Heyer's ton in this captivating love story, and helps them to escape from the inelegant bustle of modern life for a few enjoyable hours. Any lover of light Regency novels is sure to like this book.
The love story involves the rather sweet Clarice and dashing David. Even though the heroine Clarice lives in a luxurious way, she has lots of problems to overcome, including her demanding father and her new feelings for her old friend David. Her father regards David as highly unsuitable, and he is determined to marry her off to an untrustworthy, but titled man. Clarice also has to deal with the snide remarks of a 'friend', which doesn't help her lack of confidence in society circles.
When David discovers that he wants to win Clarice at all costs, he has to cope with his illegitimacy and money problems. The women from his past don't impress Clarice. Can he show her that he is worthy of her?
The likeable hero and heroine, developing love story and details of Regency life make this a good read. I especially enjoyed the descriptions of the Regency gowns and dances.
Thursday, October 02, 2014
An Unsuitable Match by Sasha Cottman
Labels: An Unsuitable Match, Georgette Heyer, Penguin, Regency, Sasha Cottman
No comments:
Subscribe to: Post Comments (Atom)


text:
Hiya Folks! I'm gearing up for my busiest time of year here at Barking Dog Salvage & Design... And unfortunately I don't have happy little elves to help me get my work done (Just 3 naughty cats and 2 pesky little dogs AND 1 big dog who likes to steal paint brushes lol!) So I'm a bit behind in my work and desk duties... I promise that I do have lots of new shutter artwork, hand-painted signs and upcycled goodies in the works but I just haven't had time to edit the photos and to list them in my Shop. I'm hoping to have the Shop updated in a few days and I wanted to thank you for your patience in the meantime. *If there is a particle design you are interested in or have any questions please do not hesitate to contact me. Thanks again for you patience, and I apologize for the delay,
Angela & the Barking Dogs
Copyright © Barking Dog Salvage and Design. All rights reserved.


text:
Reviews of Emma Brown hand painted kitchens Kent
If you have had your kitchen or furniture painted by Emma Brown could you please leave a review to help future visitors.
Click here to submit your review.
Sent by email:
I really loved having you here and am missing you already! Will recommend you highly to anyone wanting kitchens painted.
Sent via email:
My mother cannot believe the change! Loves it.
Everyone who has seen it loves it.
Thanks again for the new kitchen, we are thrilled!
Erin & R-J
Sent via email:
Hi Emma
We are very pleased with the kitchen. It looks fantastic and it's like walking into someone else's house!
Thank you so much.
Karen and Mark
Received by email:
Thank you again, Emma, for a fantastic job and we will certainly be more than happy to recommend you.
If you are over this way do pop in for a cup of coffee we would love to see you.
Wishing you all the very best.
Kind regards
Emma did an amazing job on transforming several pieces of tired pine furniture in a very dated kitchen. Very pleased with finished result and has completely up dated our country kitchen. I would highly recommend her. Thanks Emma 🙂
Kitchen refurb
In early spring Emma was commisioned to paint our kitchen cabinets, replace glass doors and also refurbish some cabinets and a Welsh dresser. The end result was extraordinary in transforming a rather tired and old fashioned kitchen into a new bright modern looking kitchen/diner. Emma's advise at all stages was spot on and the quality of her work is meticulous and highly professional and disruption kept to a minimuim. The bonus of course is that Emma was very friendly and a pleasure to have in the house. We would certainly have no hesitation in recommending Emma.
Kitchen re paint
Emma has done an excellent job bringing our old solid wood kitchen back to life and more importantly into 21st century. Her help was invaluable in advising and choosing the paint along with what elements of the kitchen needed additional cosmetic alterations - thank you
Fantastic kitchen transformation
Emma came round for an initial consultation and we immediately felt that we would be able to trust Emma to do a good job. She arranged for us to visit another client's house so we could see the standard of her painting and we were so impressed we decided to ask Emma to go ahead with painting our tired wooden cupboards in a lovely fresh cream colour.
Our kitchen has been transformed - the quality of Emma's painting is excellent and visitors have been amazed by how lovely the kitchen looks.
We would definitely recommend Emma's work to anyone wanting to update their kitchen units without replacing them.
If you want perfection, look no further than having Emma paint your kitchen or furniture. A real pleasure to have had Emma in our home for four weeks doing a meticulous job - we now want to book her for painting the study furniture.
Emma arrived at our initial consultation with lots of paint samples (many of which were painted onto wood which gave a much better indication of colour than paint charts), photos of previous kitchens and lots of ideas and advice.
She was extremely professional and accommodating once work started even though we kept postponing due to overrunning building works and was friendly and sunny throughout.
The finished kitchen looks brilliant. The quality of her work is top notch and she left the kitchen very tidy and clean at the end of each day. It has updated and smartened our old oak kitchen to the extent that visitors can't believe it's been painted, they presume we have a new kitchen.
I would have absolutely no hesitation in recommending Emma.
Traditional Painter is online home to 20 of the country's best independent kitchen & furniture painting specialists.
If you would like to employ the services of your local recommended TP kitchen & furniture painter, please use the map widget below.
Basis of a TP recommendation | Kitchens | Furniture
If the info we have shared in TP blog articles has given you the confidence to paint kitchen cabinets and furniture yourself, that is great. And in general, we hope that all homeowners, DIY and trade can take away & use our ideas for decorating better, faster or cleaner than the accepted norm.
Contact Andy at Traditional Painter for general enquiries
01603 861935


text: Would you believe me if I told you that you can turn a can of chickpeas into tuna? Okay, not actual tuna of course but it tastes pretty close. Growing up, one of the easiest lunches I knew how to make was a simple tuna sandwich. Sadly, when I transitioned to a plant-based diet that was no … Continue reading Flavorful Chickpea "Tuna" Salad


text:
You don't want to miss this.
2017 Newsletter
Happy New Year!
To start your New Year off with a bang, we have a 30% sale on selected
Siglent 2000X series powerful oscilloscopes!
These Siglent SDS2000X scopes,
with a choice of 100-300MHz bandwidth, are state-of-the-art 2-
and 4-channel desktop essentials with an impressive screen
refresh rate (140,000 wfm/s normal mode, up to 500,000 wfm/s
sequence mode), huge 140Mpt waveform memory (for drilling down
into those infrequent glitches), fast sampling (2GSa/s) and
simple operation. Most of the commonly used functions can
be accessed with a user-friendly one-button design. The
SDS2000X series employ a new generation of SPO "Super Phosphor"
technology. They feature an innovative digital trigger
system with high sensitivity and low jitter. As well as a
256-level intensity grading display function, they include a
color temperature display mode to show even more signal
information. The trigger system supports multiple powerful
triggering modes including serial bus triggering. History
waveform recording and sequence acquisition allow for extended
waveform records to be captured, stored, and analyzed. Also
included is an impressive array of measurement and math
Available options - which you can add later if you want - include
a 25 MHz arbitrary waveform generator, 16 digital channels (MSO),
as well as serial decoding.
Grab one while they're here and save!
Also – we have a
limited number of 4-channel 70MHz Rigol DS1074Z-S scopes that are
discounted due to the fact that their Calibration Certificates
have just expired. We bet they're still in calibration
(Note: the Rigol DS1074Z-S includes a self-calibration
feature: the self-calibration program can quickly make the
oscilloscope reach the best working state to get the most precise
measurement values. You can perform self-calibration at any time
especially when the change of the environment temperature is up
to or more than 5degC.) Reg. $818 Sale! $655
Company, Inc. | 585-385-1750 | 585-385-1768 |
email@example.com | Website: www.saelig.com