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Soprano Sara Macliver and mezzo-soprano Fiona Campbell's Diva's Delight two years ago at St George's Cathedral in Perth was a hit, and with 100 more tickets just released, their follow up concert Diva's Delight Again isn't one to miss. Alongside Joseph Nolan on piano, the divas – who are close friends off stage – will perform a concert ranging from Pergolesi's Stabat Mater to favourite cabaret numbers. We caught up with the pair to find out what's in store – and what keeps them singing together.
Sara Macliver and Fiona Campbell. Photo © Iris Joschko
When did you first sing together?
Sara: Alcina for Perth International Arts Festival in 1996, I played the young, innocent Morgana and Fi was the grubby boy Ruggiero.
What have been some of your most memorable moments working together?
Fiona: For me it would be Bach's St Matthew Passion, Handel's Messiah… there is no one who sings this rep better than Sara, she is my favourite soprano I've worked with in this repertoire – and our Australian Chamber Orchestra tour when we first really got to know each other on the road.
Sara: Our ACO and Australian Brandenburg Orchestra tours were really memorable, not just for the incredible music and the fun, but the genuine, deep friendship that we developed.
How would describe your dynamic on (and off!) stage?
Fiona: Professional and glamorous onstage… besties, naughty always up for a laugh and always there for each other offstage!
Sara: Onstage – symbiotic. Offstage – warm, fun and a shared wicked humour.
Sara Macliver and Fiona Campbell. Photo © Iris Joschko
Do you share similar musical tastes?
Fiona: Well I have an extremely eclectic mix in music… all good music is my mantra.
Sara: I'm a real sucker for jazz. I love the classics with Louis Armstrong and Ella Fitzgerald, but I also love artists like Madeleine Peyroux and Diana Krall.
How do you go about choosing repertoire for the two of you?
Fiona: We both choose repertoire that suits us well individually and then find duets to complement that selection. Or we have a complete work like the Pergolesi or a set of Mendelssohn duets and then find something to balance that energy in the program.
Something our listeners may not know so well is our love of cabaret. Speaking for myself here, it's my guilty pleasure and secret love! Sara and I have created a fun and fantastic selection specifically for Diva's Delight Again at St George's Cathedral. I mean, we love making people cry with sad songs, but we also really love making people laugh!
Sara: We always workshop our ideas; it's very collaborative. Sometimes we have to consider what else is on the program and other times we have the luxury of starting from scratch. It's a real treat to be able to create your own program and to choose music that really suits our voices.
What do you enjoy most about performing together?
Fiona: Everything! Sara and Joseph [Nolan] are such fabulous musicians and we've worked together so often now that we feel very secure on stage together. This implicit trust in one other allows us to focus on the music and emotions, and delivering a seriously exciting performance for our audience.
Sara: The absolute joy of making incredible music together. It's an absolute privilege.
Sara Macliver and Fiona Campbell. Photo © Iris Joschko
How do you like to unwind after performances?
Fiona: Champagne and riotous laughter with friends and colleagues is de rigeur.
Sara: Drinking!! (Is that bad?!)… And eating… always together!!
What can the audiences expect from Diva's Delight Again? Will there be any surprises?
Fiona: We have put this cabaret selection together exclusively for the Cathedral, so there will definitely be things people haven't heard us sing together before. Most audiences know us for our classical music careers and the Pergolesi in the first half is simply divine, glorious vocal writing at its best.
It's hard not to blow our own baroque trumpet here, but you simply won't hear it sung better, because our voices blend and move together in a way that creates musical magic. And of course there's a big finish because you simply can't have two divas onstage without a bit of rivalry. We shall see who wins this duet… (my money's on the mezzo!)
So it's all a bit of a rollick, totally gorgeous and we promise you will leave the Cathedral dancing in the aisles… we may even get you singing along!
Sara: I think some people will be surprised to hear us sing cabaret! I hope that with the Pergolesi they are transported because the music is simply sublime and I hope with the cabaret that they can share the joy, fun and beauty of this great repertoire.
Diva's Delight Again is at St George's Cathedral, Perth, August 3


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Parenting: Guiding Principles for Highly Successful Parenting
August 12 @ 1:00 pm - 3:00 pm| Main Library (Traverse City)
Event Navigation
Parents are invited to join MSU Extension instructor, Tracy Trautner, for this 4 week series.
The early years of learning and behavior establish patterns that impact the rest of your child's life. During this time, the developing brain becomes ready for learning, patterns of behavior and self-concept are well-established, and a child builds a foundation for a lifetime of learning. This 4 week series will explore ways that families can be highly effective in raising their kids to be successful in school and in life.
The 4 sessions of this program run on Mondays from 8/5 – 8/26 and will cover the following topics:
- August 5th: Developing family routines
- August 12th: Developing emotional control in your child
- August 19th: Being a calm, assertive parent
- August 26th: Learning from mistakes and developing empathy & the ability to be thankful as a result
Sign-up HERE or drop in to participate. Childcare is not provided.
"20180903-IMG_1134" by francis.aldana is licensed under CC BY-NC-SA 2.0

subset: commoncrawl


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CasinoLuck – Lucky 15 promotion
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Pretty Personalized Paris, Eiffel Tower, Damask Handmade Wall Plaque
Plaque à la Main Personnalisée de Paris Sur le Thème Du Mur!
it's wonderful to see more Paris themed decor items being made the old world way by hand, so you know it's unique to you or the person being gifted!
This magical black and white damask, Eiffel Tower wall plaque, highlighted by the recipients name in trendy teal and embellished so beautifully with black ribbon and bows, is hand-crafted using a durable, sturdy, vinyl PVC plastic with reinforced metal corners where the black bows are attached. There is a tick of approval for workmanship as this elegant door sign takes approx 3-4 days to create by artisans who clearly understand, how to create a wall plaque that will be a delight for Paris fans.
Unique Personalized Paris Themed Gift
You have choices when ordering including the size (small, medium, large), name and color plus you can add the word "room" to the text. You can also select font type and ribbon color.
This glamorous wall plaque will look gorgeous on your dorm or bedroom door, announcing to all who wish to enter, how much you truly love the city of light!


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Baby & Kids Feeding & Mealtime Baby Dishes, Cups & Bibs Bibs & Burp Cloths Lassig Little Chums Eva Bib Mouse
Lassig Little Chums Eva Bib Mouse
Lassig Little Chums Eva Bib Mouse
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Wipeable bibs made by Lässig for little discoverers. Not only the name is the order of the day, also the neat, child-oriented patterns will reveal a smile on adults and infants. The little ones at the age of 6 to 24 months will truly feel comfortable on their feeding expedition, because not only the self feeding experiments are allowed to be a complete flop. If something is missing its target then the cloths will still be safe due to the additional flip pocket to collect things and by this means offering the perfect start through the day.
- Additional flip pocket
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YAIAA athletes competed in the broad jump, shot put and a relay race during spring sports media day on March 3, 2018. Dustin Levy, The Evening Sun
It's Day 2 of GameTimePA YAIAA spring media day, this time coming to you from the York Daily Record office.
Follow along as GameTimePA staff ask questions, take photos and test athletes and coaches with a variety of games.
If the updates don't come through, please refresh your browser.
Using a mobile device? Click here.


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In the acute stage of infection following sexual transmission of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV), virus-specific CD8+ T-lymphocyte responses partially control but do not eradicate infection from the lymphatic tissues (LTs) or prevent the particularly massive depletion of CD4+ T lymphocytes in gut-associated lymphatic tissue (GALT). We explored hypothetical explanations for this failure to clear infection and prevent CD4+ T-lymphocyte loss in the SIV/rhesus macaque model of intravaginal transmission. We examined the relationship between the timing and magnitude of the CD8+ T-lymphocyte response to immunodominant SIV epitopes and viral replication, and we show first that the failure to contain infection is not because the female reproductive tract is a poor inductive site. We documented robust responses in cervicovaginal tissues and uterus, but only several days after the peak of virus production. Second, while we also documented a modest response in the draining genital and peripheral lymph nodes, the response at these sites also lagged behind peak virus production in these LT compartments. Third, we found that the response in GALT was surprisingly low or undetectable, possibly contributing to the severe and sustained depletion of CD4+ T lymphocytes in the GALT. Thus, the virus-specific CD8+ T-lymphocyte response is "too late and too little" to clear infection and prevent CD4+ T-lymphocyte loss. However, the robust response in female reproductive tissues may be an encouraging sign that vaccines that rapidly induce high-frequency CD8+ T-lymphocyte responses might be able to prevent acquisition of HIV-1 infection by the most common route of transmission.
In the natural history of sexual transmission of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV), systemic infection is established throughout the lymphatic tissues (LTs) within the first weeks after exposure. Viral production and viremia peak at this time and then decline, temporally coincident with the development of virus-specific CD8+ T-lymphocyte responses (5, 14, 16, 24). However, in contrast to many other viral infections, the immune system is unable to completely clear infection. Depletion of CD4+ T lymphocytes, which is particularly severe in the gut-associated lymphoid tissue (GALT) (6, 19, 29, 33), is demonstrable in this acute stage in both HIV-1 and SIV infections and will generally progress in all LT compartments to levels where AIDS-associated opportunistic infections and cancers are manifest unless infection is controlled with antiretroviral therapy.
Understanding why HIV and SIV win this struggle with host defenses might provide important insights into designing an effective HIV vaccine. To that end, we investigated three hypothetical explanations for the failure to prevent infection and CD4+ T-lymphocyte loss following vaginal exposure. (i) The female reproductive tract is a poor inductive site to mount a virus-specific immune response and cannot thus prevent dissemination and establishment of systemic infection. (ii) The host mounts a robust response but not in enough time to prevent widespread systemic dissemination and establishment of a persistent infection in LTs. (iii) The response is of insufficient magnitude relative to the number of infected cells to fully contain infection.
We investigated these hypotheses with the SIV rhesus macaque model of vaginal transmission of HIV-1 by determining the relationship between the immune response in tissue compartments and the kinetics and magnitude of infection in those compartments described in the accompanying report. We focused on the virus-specific CD8+ T-lymphocyte response because of several lines of evidence that suggest that these responses partially control viral replication during acute infection. (i) Antigen-specific T lymphocytes are temporally associated with a reduction in plasma viremia (5, 14, 16, 24). (ii) CD8 depletion results in high-level viral replication (18, 25). (iii) The emergence of escape variants indicates selective pressure exerted by the CD8+ T lymphocytes (3, 22). We specifically investigated the Mamu-A*01-restricted immunodominant epitopes, Gag181-189CM9 and Tat28-35SL8, because the response to these two epitopes accounts for the majority of detectable CD8+ T-lymphocyte responses in Mamu-A*01-positive macaques (21) and because Gag CM9-specific responses following intravenous transmission have been documented in the mucosal and lymphoid tissue compartments which are relevant to understanding the reasons why immune defenses fail to fully protect animals following intravaginal exposure. Veazey et al., for example, detected Gag CM9-specific CD8+ T lymphocytes in the blood and the intestinal mucosa at 2 and 3 weeks postintravenous infection at similar frequencies (30, 32). During the chronic phase of infection, virus-specific CD8+ T lymphocytes were detected at similar levels in peripheral blood mononuclear cells (PBMC) and secondary lymphoid tissues (16), and tetramer-staining lymphocytes were found at comparable, or higher, frequencies in the vagina and intestinal mucosa (26, 28, 32).
Here, we show first that CD8+ lymphocytes in female reproductive tissues do respond to infection at the portal of entry, but there is a surprisingly long lag between CD8+ T-lymphocyte responses to the immunodominant SIV epitopes and peak levels of infection at the portal of entry and all LT compartments. Second, the magnitude of the virus-specific CD8+ T-lymphocyte responses, while relatively robust at the portal of entry, was modest in LTs generally and low or undetectable in the GALT. The overall disparity in the timing and magnitude of immunodominant response with respect to propagation and dissemination of infection is thus a major factor in the failure to clear infection and prevent CD4+ T-cell loss, particularly preferential depletion of CD4+ T lymphocytes in the GALT.
Monkeys, SIV inoculum, and infection status.As described in the accompanying paper by Miller et al. (19a), eight Mamu-A*01 rhesus monkeys became systemically infected after intravaginal inoculation.
Isolation of PBMC.PBMC were isolated from heparin- or EDTA-treated blood samples using Lymphocyte Separation medium (ICN Biomedicals, Aurora, OH). PBMC not used in fresh assays were frozen in 10% dimethyl sulfoxide (Sigma, St. Louis, MO)-90% fetal bovine serum (Gemini BioProducts, Calabasas, Calif.) and stored in liquid nitrogen for future analyses.
Lymphoid tissue collection and lymphocyte isolation.At necropsy, blood, intestine, spleen, and peripheral and genital lymph node (LN) samples were collected. In addition, cell suspensions were prepared as previously described (17) from whole blood, spleen, and peripheral and genital lymph nodes for gamma interferon (IFN-γ) enzyme-linked immunospot assay and fluorescence-activated cell sorter (FACS) analysis by gentle dissection of lymph nodes with scalpels in RPMI 1640 (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini BioProducts, Calabasas, CA) (complete RPMI) and passage of the cell homogenate through a cell strainer (Fisher, Pittsburgh, PA). The cells were washed twice by centrifugation for 10 min at 1,600 rpm. Spleen tissue samples were cut into small pieces and homogenized with a syringe plunger. The homogenate was passed through a cell strainer. Splenic lymphocytes were isolated by gradient centrifugation with Lymphocyte Separation medium from ICN Biomedicals (Aurora, OH), followed by two washes with complete RPMI. PBMC were isolated from whole blood with Lymphocyte Separation medium (ICN).
Isolation of lymphocytes from the cervix and vaginal mucosa.At necropsy, fresh cervix and vagina were collected in complete RPMI medium containing 10% fetal calf serum, 2 mM l-glutamine, 100 U/ml penicillin, 0.1 mg/ml streptomycin (Sigma, Inc., St Louis, MO,) and 1% amphotericin B (Sigma). Lymphocytes were isolated as previously described (17) from genital mucosal tissues by cutting the tissues into small pieces (maximum size, 10 by 10 by 5 mm) and placing them in 1.2 U/ml Dispase II (Boehringer Mannheim GmbH, Germany) in complete RPMI medium (3 ml per piece). The tissue-dispase II mixture was incubated in a water bath shaker (New Brunswick Scientific, Inc., Edison, NJ) for 2 h at 37°C and 200 rpm. After being rinsed, the epithelial layer was removed from the underlying tissue with forceps and scalpel. The subepithelial tissues were cut into 2- by 5-mm slices (each, approximately 2 mm thick) and incubated in cell release medium. Cell release medium is complete RPMI 1640 containing 25 mM HEPES (Sigma), 5 × 10−3 M β-mercaptoethanol (Sigma), 0.5 mg/ml collagenase (Sigma), 0.1 mg/ml DNase (Sigma), and 0.02 mg/ml ciprofloxacin-HCl. The tissue was incubated for 3 to 4 h in a shaking water bath at 37°C and 100 rpm. To obtain cell suspensions from the lamina propria, 40 ml of the submucosal tissue suspension was shaken vigorously in a tube for 90 s. The resulting cell suspension was then passed through a 100-mesh sterile stainless steel sieve to remove larger pieces of debris and centrifuged at 530 × g for 10 min. Mononuclear cells were subsequently isolated on a discontinuous Percoll (Pharmacia) gradient. Cells were resuspended in 40% Percoll in RPMI medium, layered onto 75% Percoll in phosphate-buffered saline (PBS), and centrifuged at 530 × g for 30 min using the no-brake setting. Cell viability, measured by trypan blue exclusion, was typically >95%.
Lymphocyte isolation from uterus.After being extensively washed in PBS, the uterus was cut into 4- to 6-mm2 pieces. The tissues were digested in R10 (RPMI 1640 supplemented with 10% fetal calf serum [FCS], 2 mM l-glutamine, 25 mM HEPES buffer, 50 μg/ml streptomycin, 50 U/ml penicillin) containing type IV collagenase (0.5 mg/ml) (Sigma) and DNase type IV (100 U/ml) (Sigma) for 2 h at 37°C. The supernatant was collected, and lymphocytes were enriched by density gradient centrifugation using a 90% to 40% isotonic Percoll gradient at 1,200 rpm for 30 min at room temperature. The lymphocytes were collected from the 90% to 40% Percoll interface. Cells were washed in R10.
Isolation of lymphocytes from GALT.Sections of colon and jejunum, 6- to 8-in long, were cut into approximately 5-mm2 pieces and incubated in complete Hank's balanced salt solution (Sigma, St. Louis, MO) supplemented with 10% FCS, 25 mM HEPES buffer, and 1 mM EDTA at 37°C for 90 min. Supernatant, containing intraepithelial lymphocytes (IEL), was collected and run over glass wool loosely packed into a 30-ml syringe to remove large particulates. Flowthrough was washed twice with 30 ml of R10. To release the lamina propria lymphocytes (LPL), the remaining tissue was cut into small pieces of ∼1 mm2 each and incubated for 90 min at 37°C in R10 containing type IV collagenase (15 U/ml) (Sigma, St. Louis, MO) and DNase type IV (100 U/ml) (Sigma, St. Louis, MO). Supernatant was collected, run over loosely packed glass wool, and washed twice in 30 ml of R10. Both IEL and LPL were enriched from epithelial cells by centrifuging over a 90% to 40% isotonic Percoll gradient. Lymphocytes were collected from the 90% to 40% Percoll interface.
Intracellular cytokine staining of fresh lymphocytes.Intracellular cytokine (ICS) assays were performed as previously described (12). Briefly, 5 × 105 to 1 × 106 freshly isolated lymphocytes were incubated with individual peptides at a concentration of 5 μg/ml along with 0.5 μg anti-CD28 (clone L293; BD Biosciences, San Diego, CA) and 0.5 μg anti-CD49d (clone 9F10; BD Pharmingen, San Diego, CA) for optimal costimulation in a total volume of 200 μl R-10. Staphylococcal enterotoxin B (10 μg/ml; Sigma, St. Louis, MO) was used as a positive control while R10, anti-CD28, and anti-CD49d alone were used as a negative control. After incubation for 1.5 h at 37°C 10 μg/ml of brefeldin A (Sigma, St. Louis, MO) was added to inhibit secretion of cytokines. The cells were further incubated for 5 h at 37°C. Cells were washed twice with FACS buffer (PBS plus 2% FCS) and stained with anti-CD8α-PerCP (clone SK1; Becton Dickinson) and anti-CD4-APC (clone SK3; Becton Dickinson) in 100 μl FACS buffer for 40 min at room temperature. After two washes, the cells were fixed with 2% paraformaldehyde (PFA)-PBS solution overnight at 4°C. After being washed twice with permeabilization buffer (0.1% saponin in FACS buffer) the cells were stained with anti-human IFN-γ-fluorescein isothiocyanate (FITC) monoclonal antibody (MAb) (clone 4S.B3; Pharmingen) and anti-human tumor necrosis factor alpha (TNF-α)-PE MAb (clone Mab11; Pharmingen) and incubated for 50 min at room temperature. Afterwards, the cells were washed twice with permeabilization buffer and fixed with 2% PFA-PBS. A total of 10,000 to 200,000 lymphocyte-gated events were acquired on a FACSCalibur flow cytometer (Becton Dickinson) and analyzed with FlowJo software (Treestar). All values are reported after subtraction of the background level staining.
Tetramer staining and FACS.From 5 × 105 to 1 × 106 freshly isolated lymphocytes were suspended in a 100-μl volume of R10 and stained for 1 h at 37°C with Mamu-A*01/peptide tetramer labeled with phycoerythrin or antigen-presenting cell. After a 1-h incubation, the lymphocytes were stained for surface markers using anti-human CD3ε-FITC (SP34; Pharmingen) and anti-CD8α-PerCP (clone SK1; Becton Dickinson) and incubated for an additional 40 min at room temperature. The cells were washed twice with FACS buffer and fixed with 2% PFA-PBS. Sample data was acquired using a FACSCalibur flow cytometer (Becton Dickinson) and analyzed using FlowJo software (Treestar). Background staining of CD3−/CD8+ lymphocytes in all tissues was typically ≤0.1%.
In situ tetramer (IST) staining.Biotinylated Mamu-A*01/β2m/peptide molecules were produced with Gag (CTPYDINQM), Tat (TTPESANL), or irrelevant (FLPSDYFPSV) peptides at the National Institute of Allergy and Infectious Diseases tetramer facility. Tetramers were generated by adding six aliquots of FITC-labeled ExtraAvidin (Sigma) to biotinylated Mamu-A*01/β2m/peptide monomers over the course of 8 h to a final molar ratio of 4.5:1. IST staining was performed essentially as previously described (27). Fresh lymph nodes, spleen, uterus, and vaginal and cervical tissues were shipped on ice overnight in PBS or PBS with 100 μg/ml heparin (PBS-H). Tissues were cut into 0.5-cm pieces and embedded in 4% low-melt agarose. Tissue blocks were placed in a vibratome bath containing 0 to 4°C PBS-H, and 200-mm-thick sections were generated. Fresh sections were stained free floating in 1 ml of solution with four sections per well in 24-well tissue culture plates. Incubations were carried out at 4°C on a rocking platform. Tetramers were added at a concentration of 0.5 mg/ml with 2% normal goat serum (NGS) and 0.5 mg/ml mouse anti-human CD8 antibodies (Dako clone DK25) and incubated overnight. Sections were washed with chilled PBS-H and then fixed with 4% paraformaldehyde for 2 h at room temperature. Sections were again washed with PBS-H, incubated with rabbit anti-FITC antibodies (BioDesign) diluted 1:10,000 in PBS-H with 2% NGS, and incubated for 1 to 3 days. Sections were washed three times with PBS-H for at least 20 min and then incubated with Cy3-conjugated goat anti-rabbit antibodies and Alexa 488-conjugated goat anti-mouse antibodies (Jackson ImmunoResearch), both diluted 1:1,000 in PBS-H with 2% NGS, for 1 to 3 days. Finally, sections were washed three times for at least 20 min in PBS-H, postfixed with 4% paraformaldehyde for 1 h, and then mounted on slides with warmed glycerol gelatin (Sigma) containing 4 mg/ml n-propyl galate. Stained sections were analyzed using a Bio-Rad 1000 confocal microscope.
CD8+ T-lymphocyte responses to immunodominant SIV epitopes lag behind peak levels of virus production at the portal of entry and LTs.In the accompanying report, we show that infection is established beyond the portal of entry in the proximal and distal LTs by the end of the first week postintravaginal inoculation (PI). We found that viral replication peaked in all tissues and blood between days 10 and 14 PI and then declined by an order of magnitude or more by day 28 PI, the last time point examined.
Here, we investigated the relationship between propagation and spread of infection and the development of SIV-specific CD8+ T-lymphocyte responses to the Mamu-A*01-restricted immunodominant epitopes in Gag (Gag CM9) and Tat (Tat SL8) that comprise the majority of the detectable virus-specific CD8+ T-lymphocyte responses during acute infection of Mamu-A*01+ macaques (21). To make optimal use of Mamu-A*01+ macaques, we first bracketed the times virus-specific cellular immune responses were detectable in Mamu-A*01− macaques by an optimized enzyme-linked immunospot assay shown previously to be comparable in sensitivity to flow cytometric assays (23). We did not detect significant cellular immune responses until day 14 PI and then in only one of the two animals. By 21 days PI, virus-specific cellular immune responses were detectable in the reproductive and secondary lymphoid tissues (not shown). Therefore, we focused the detailed compartmental analysis in Mamu-A*01+ macaques at 13 to 28 days PI, spanning the peak through decay stages of declining viral loads and productively infected cells (19a).
We determined the location and magnitude of epitope-specific T lymphocytes by collecting tissue and isolating lymphocytes from the female reproductive tract (vagina, cervix, and uterus), the colon and jejunum, the proximal lymphoid tissues draining the site of inoculation (genital and inguinal lymph nodes), the distal lymphoid tissues (mesenteric lymph nodes, axillary lymph nodes, and spleen) and the blood. CD8+ T-lymphocyte responses against Gag CM9, Tat SL8, and two previously reported Mamu-A*01 restricted subdominant responses, Env233-241CL9 and Env620-628TL9 (10), were detected by in situ staining and FACS analysis using Mamu-A*01/peptide tetrameric complexes. Peptide-specific cytokine secretion was monitored by ICS using antibodies against IFN-γ and TNF-α. We present only data for responses against Gag CM9 and Tat SL8, because measurable responses to the Env epitopes were not detectable. Additionally, both IEL and LPL were isolated from the colon and jejunum, but little or no difference in the frequency of epitope-specific CD8+ T-lymphocyte responses was detected between these compartments. We therefore present only the responses from the LPL.
At 13 to 14 days PI, 4 days after the peak levels of virus in female reproductive tissues (19a), we detected low but significant responses against the Gag CM9 and Tat SL8 epitopes in the reproductive tract and in blood, but not elsewhere (Fig. 1) in one of two animals. At days 20 to 21 PI, we detected Gag CM9- and Tat SL8-specific responses at different levels in the female reproductive tract tissues and lymphatic tissue compartments in the three animals tested (Fig. 2 and Fig. 3A). The tissues of the female reproductive tract consistently had high frequencies of Gag CM9- and Tat SL8-specific CD8+ T lymphocytes (range, 1 to 5%, with a high of 24%). In contrast to this relatively robust response at the site of initial exposure, there were only modest responses in the draining and distal LNs and very low or undetectable responses in the GALT (Fig. 3A). Similarly, at days 27 to 28, the frequencies of tetramer-positive cells were highest in the reproductive tissues, followed by the draining and/or distal LNs, and relatively low in the colon and jejunum (Fig. 3B). IST staining of lymph nodes, spleen, vagina, and cervix from each animal similarly revealed Gag CM9- and Tat SL8-specific CD8+ lymphocytes in reproductive and lymphoid tissues at 20 to 21 and 27 to 28 but not 13 to 14 days PI (Fig. 4). Thus, particularly in GALT, the level of immunodominant response not only lagged well behind the peak of viral replication but was also the lowest of any LT compartment.
We found no reproducible difference in the distribution pattern of the CD8+ T-lymphocyte responses against the two-immunodominant SIV epitopes in terms of their frequencies among the different tissue compartments. Furthermore, we found no evidence for higher epitope diversity with respect to the four SIV epitopes in the reproductive tissues and regional lymph nodes investigated compared to other mucosal sites or lymphoid tissues (data not shown).
Homing of IFN-γ-secreting SIV-specific CD8+ T lymphocytes to the cervicovaginal mucosa.Because insufficient numbers of cells were recovered to directly measure cytotoxicity of freshly isolated CD8+ T lymphocytes from the female reproductive tissues and GALT, we evaluated the effector status and location of the SIV epitope-specific lymphocytes by ICS for production of IFN-γ and TNF-α. We detected cytokine-secreting cells in all but one of the animals, in larger numbers in the female reproductive tract than in the LNs and the GALT (Table 1), as illustrated for animal 27572 (Fig. 5). We speculate that the effects hormonal changes during the menstrual cycle have on effector function (8, 35) may be the reason that we detected tetramer-positive cells but not cytokine-secreting cells in one animal (24225).
In the accompanying report, we show that systemic infection is quickly established throughout the LTs and show in this report that this occurs before a significant virus-specific CD8+ T-lymphocyte response to the major immunodominant SIV epitopes has developed. Robust CD8+ T-lymphocyte responses against two immunodominant epitopes are detected only after the peak of viremia and then mainly in the female reproductive tract but not in the GALT. Moreover, while strong responses were detected in the three animals infected for 20 to 21 days, lower frequencies of tetramer-positive cells were observed in two of the three animals infected for 27 to 28 days, consistent with declining virus-specific CD8+ T-lymphocyte responses from a peak earlier in infection. The strikingly different virus-specific CD8+ T-lymphocyte response in the female reproductive tissues and the GALT is consistent with an increasing body of evidence that suggests that the mucosal immune system is a distinctly compartmentalized rather than a single system (15, 20, 34). We found relatively poor virus-specific responses in the GALT after intravaginal inoculation, in contrast to a previous study where relatively high frequencies of Gag CM9-specific CD8+ T lymphocytes were detected in the GALT during acute infection after intravenous inoculation (30). In our study, virus-specific CD8+ T lymphocytes were concentrated in the female reproductive tract. The relatively poor CD8+ T-lymphocyte response in GALT, compared to the response in the female reproductive tract, may be due, at least in part, to the preferential homing of virus-specific effector cells back to the initial site of infection (20). This would account for the higher frequency of epitope-specific responses in the female reproductive tissues and the decreased frequency of responses at more distal sites. For two reasons, we think that it is unlikely that the explanation for the low response is the loss of CD8+ T lymphocytes during isolation because of the susceptibility of GALT lymphocytes to undergo apoptosis. (i) The proportion of CD8+ T lymphocytes isolated from the intestinal tissues was high (65 to 90%). (ii) Using these same isolation procedures, we have detected high frequencies of tetramer-positive cells in GALT in chronically infected animals and in the acute stages of infection in animals infected intrarectally (unpublished data), similar to results previously described by other investigators (26).
The preferential depletion of CD4+ T lymphocytes in the GALT by SIV and HIV may reflect infection of larger numbers of susceptible CD4+ T-lymphocyte targets (2, 4, 31, 33). However, the poor to nonexistent virus-specific CD8+ T lymphocytes documented here may be an important contributing factor in the preferential CD4+ T-lymphocyte depletion in GALT.
The robust immune response following intravaginal inoculation was somewhat unexpected in light of a recent report that a recombinant poxvirus strain of modified vaccinia virus Ankara failed to elicit CD8+ T-lymphocyte responses in the female reproductive tract of mice when the vaccine was administered intravaginally but did elicit a response after intranasal inoculation (11). Here, we show that the lower female reproductive tract in primates can be an excellent antigen-specific CD8+ T-lymphocyte-priming site when exposed to replication-competent SIV.
This compartmental analysis reveals some of the potential reasons virus-specific CD8+ T-lymphocyte responses fail to fully contain infection in the LTs targeted by SIV and, by extension, HIV, because of the relatively low frequency of virus-specific CD8+ T lymphocytes in LTs after peak virus production. However, a vaccine-induced local recall response might control viral replication before the immune system must contend with large numbers of infected cells distributed throughout the LTs. Evidence that a local response might prevent transmission comes from studies in which antigen-specific IFN-γ-producing CD8+ T lymphocytes were detected in cervical tissue samples of women exposed to seropositive partners who remain seronegative with no detectable plasma viral loads (13).
In summary, we show here that SIV replicates to peak levels unchecked by a CD8+ T-lymphocyte response against immunodominant SIV epitopes for at least 1 week and, in the GALT for an additional 2 weeks following intravaginal transmission. CD4+ T lymphocytes in GALT thus lack protection from infection and the cytopathic effects of SIV replication for an extended period, which may be one reason that CD4+ T-lymphocyte depletion is greater in GALT than in other lymphoid organs. In this experimental model of the natural history of intravaginal transmission and acute infection, the immune response is too little, too late, just as it may be in preventing the establishment of a persistent HIV-1 infection (9). To be successful, a vaccine would clearly have to induce a much more rapid and robust recall response, not only in the reproductive tissues where infection begins but also throughout all LT compartments. Nonetheless, the robust response in female reproductive tissue is an encouraging sign that it may be possible to develop an effective vaccine that limits infection at the portal of entry.
We thank the Immunology Core Laboratory and Primate Services Unit of the California National Primate Research Center (CNPRC); the staff at the Wisconsin Primate Research Center (WPRC); Ding Lu, Tracy Rourke, Rino Dizon, and Blia Vang for technical assistance; and Colleen O'Neill and Tim Leonard for help in preparing the manuscript and figures.
This work was supported by NIH grants R01 AI48484 to A.H., U51 RR00169 to the CNPRC, R01 AI51239 and R01 AI51596 to C.J.M., and P51 RR00167 to the WPRC.
- Received 6 December 2004.
- Accepted 2 March 2005.
- *Corresponding author. Mailing address for Ashley Haase: Department of Microbiology, University of Minnesota, MMC 196, 420 Delaware St. S.E., Minneapolis, MN 55455. Phone: (612) 624-4442. Fax: (612) 626-0623. E-mail: haase001{at}umn.edu. Mailing address for David I. Watkins: Wisconsin Primate Research Center, 1220 Capitol Ct., Madison, WI 53715. Phone: (608) 265-3380. Fax: (608) 263-4031. E-mail: Watkins{at}primate.wisc.edu.
↵† Present address: Rio Grande City High School, Rio Grande City, TX 78582.
- American Society for Microbiology


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Painted Cows Wood Wall Decor
Accent your sweet farmhouse with color! Painted Cows Wood Wall Decor features a colorful painted MDF design of three cows in black, brown, and blonde colors. Moo-ve your existing decor to make room for this beauty!
- Length: 16"
- Width: 20"
- Thickness: 1 1/2"
Hanging Hardware: 2 - D-Rings (19 3/8" Apart from Center to Center)
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Watch the forum (Previously recorded)
Stockbridge, MA, January 29, 2016—In celebration of the 75th anniversary of President Franklin Delano Roosevelt's "Four Freedoms" speech, Norman Rockwell Museum's Four Freedoms Forum series returns on Thursday, February 18, at 5:30 p.m., with a look at the current state of presidential politics. The evening's forum will address the ins and outs of the 2016 American presidential election cycle, its colorful candidates, and what it means for the future of the nation. Featured speakers will include: William "Smitty" Pignatelli, Democratic member of the Massachusetts House of Representatives; Stacy L. Wallach, attorney and professor; Dr. Steven Lomazow, FDR Presidential Scholar and member of the Museum's National Council; and Tammy Daniels, managing editor of iBerkshires.com. The program is being sponsored by Dr. and Mrs. Steven Lomazow. Community conversation at the Four Freedoms Forums is free and open to the public.
William "Smitty" Pignatelli is the Democratic member of the Massachusetts House of Representatives, representing the 4th Berkshire District, consisting of the towns of Alford, Egremont, Great Barrington, Lee, Lenox, Monterey, Mount Washington, New Marlborough, Otis, precinct 5B of the city of Pittsfield, the towns of Sandisfield, Sheffield, Stockbridge, Tyringham and West Stockbridge in Berkshire County; and the towns of Blandford, Chester and Tolland in Hampden County.
Stacy L. Wallach is an adjunct professor at Pace University Law School in White Plains, NY, and history lecturer at OLLI at Berkshire Community College, Pittsfield, MA. Professor Wallach spent 18 years as a business, commercial, and real estate litigator with the law firm of Tenzer Greenblatt Fallon & Kaplan, and he then spent 18 years as a business executive; with the Edward S. Gordon Co. Inc. where he was general counsel, senior vice president, and member of its board of directors; with Insignia/ESG Inc where he was general counsel and senior vice president; with Cushman & Wakefield Inc, where he was senior operations director for C&W's New York Metro Region; and with CB Richard Ellis Inc, where he was senior managing director of that firm's New Jersey operations.
Dr. Steven Lomazow is a board-certified neurologist in Belleville, New Jersey. He is assistant professor of neurology at the Mount Sinai School of Medicine, a member of the New Jersey State Board of Medical Examiners, and former president of the Neurological Association of New Jersey. He received his medical degree from Rosalind Franklin University of Medicine and Science. He is the co-author of FDR's Deadly Secret, which looks at the late President's medical history. A collector and researcher of Norman Rockwell's book illustrations, the contribution of his extensive collection in 1979 to Norman Rockwell Museum is noted in Norman Rockwell: A Definitive Catalogue by Laurie Norton Moffatt.
Tammy Daniels is the managing editor of iBerkshires.com, a website devoted to Berkshire County. She has covered politics, education, community events, and breaking news throughout Northern Berkshire and Southern Vermont.
About Four Freedoms Forums:
Town Hall Meetings at the Norman Rockwell Museum
Join us to share your thoughts on the most compelling issues of our day. This series of Town Hall conversations inspired by Norman Rockwell's Four Freedoms paintings will explore aspects of our democracy and important social concerns in a rapidly changing and increasingly global world. Noted commentators will offer observations and inspire community discourse, with a reception to follow.
The tradition of Town Hall meetings has it roots in the founding of our nation where small New England communities would gather to invite citizen opinion and vote on matters of importance to the town. A town meeting is a form of direct democratic rule, used primarily in portions of the United States since the 17th century, in which most or all the members of a community come together to legislate policy and budgets for local government.
The Four Freedoms Forums are free and open to the public.


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James Watts
– After more than thirteen years working in the commercial HVAC industry, Mr. Watts left to pursue employment in the non-profit world. He served at Cobb Pregnancy Services (CPS) for three years as Development Coordinator and later served on the Board of Directors including two years as Vice-Chair. He left CPS in 2010 to serve with Life Training Institute (LTI) as the Vice-President of Development and Communications. He also spoke and wrote for the organization. He has been an invited speaker to groups at Harvard, UNC – Chapel Hill, NC State, and University of Illinois to name only a few. He has appeared on many radio and television interviews, regularly spoken as the keynote at banquets and conferences, and published articles in periodicals like The Christian Research Journal on a wide array of topics pertaining to bioethics and the university culture.
Chad Holland
– Mr. Holland graduated with a degree in Political Science from Kennesaw Sate University. He worked on staff in many political campaigns including the gubernatorial campaigns of Governor Sonny Perdue who appointed Mr. Holland as his Legislative Director for his first term as the Governor of Georgia. He served as the Executive Director of the West Virginia Republican Party for two years. He now works with his father running a high-end luxury resort development company. He has experience on both boards of directors and organizing grass roots fundraising efforts.
Rob Roux
– Mr. Roux graduated with a degree from Wake Forest University and is one of the founders and chief officers of Georgia Air Associates, a factory supply representative for the state of Georgia in commercial HVAC. He has experience serving on the boards of non-profit ministries in the Marietta, Georgia community. He is also one of the founding members and elders of Marietta Community Church.
Susan Cary
– Ms. Cary graduated from Georgia Institute of Technology with a degree in Industrial Engineering. She then co-founded Information Intellect where she operated as Lead Consultant/Project Manager.As all founders, she managed multiple areas including the installation and customization of proprietary Tax Software for Public Utility clients, developing and coding new modules for the software, and leading training for clients. Ms. Cary serves a key role in MHM safeguarding the financial and operational transparency of this organization to ensure we continue to serve the public trust with excellence.
Maina Mwaura
– Mr. Mwaura graduated from Liberty University and worked for two decades as a youth minister in multiple churches. He also worked in cooperation with other organizations to heal racial divides in our communities with a special emphasis on the Christian church community. He conducts interviews for Christianity Today's Pastor Website in which he has interviewed such people as former President Jimmy Carter and Pastor Tim Keller. Mr. Mwaura's wisdom, relationships, and character are invaluable to helping this organization pursue its mission.
Michael Rupert
– Mr. Rupert graduated with a Bachelors degree in Economics and a Masters in Government. After beginning his professional career selling commercial insurance, Mr. Rupert moved on to serve as a consultant to non-profits. He currently works as a business consultant focused on helping clients with business strategy, change management, and holistic management application.


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She came to see me. Sheila was her name: "It's always the same. I get motivated for a few weeks, lose weight, then something happens and I pile it all on again and more! I'm getting married in three months! You'd think that would be motivation enough but my weight's been even more all over the place lately!"


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We had some friends over last night and were sharing with them a lot of things about the last several months and while talking with them I felt that I needed to share a part of our conversation with you. This is stuff that has been in previous blogs, but I need to pull from the past and add some of the present so that you will get a picture of the situation and be encouraged as I was.
Back in September, right before being discharged from the PCCU (on our way to the regular floor) at Vandy we had a care conference with all the Drs. and therapists there. What they told us basically was that what we saw in Micah then was what we were going to be stuck with and that it would not get better and would most likely get worse. At that point, Micah sometimes would make eye contact (but usually not), he made no purposeful movement, he showed absolutely no emotion except pain once in a while, you get the picture... The Drs. actually told us that we should not go to the in-patient re-hab as they would do nothing for him.
Well, Micah's eyes miss nothing & he uses them to answer questions, he lifts his left leg to help every time we start to put on pants, socks, shoes, etc... and tries with the right, but it is harder for him to use, he will bump knuckles with his brothers, he laughs (at people, TV, jokes, etc...) smiles, cries, makes noise when he wants to get out of his room..... I would say that he has already exceeded the Vandy Drs. expectations for him! So for this, we thank the Lord because we know that God is not done with our son yet.
"being confident of this very thing, that He who has begun a good work in you will complete it until the day of Jesus Christ; " Philipians 1:6
God is not done with any of us yet, thank goodness! I'm glad to know that He is not going to leave me as I am, but is always leading me in a better way, raising me up to walk with Him and teaching me to know and do His will. God has a purpose for each of us, and He is constantly drawing us towards that purpose. Some things we are all called to do (love our brother & sisters, be a servant, etc...), but then we each have our own calling also and it is ours to fulfill and no one can do it for us. We each must be confident in the Lord that He will not leave us without help, but will strengthen and give us all that we need to fulfill His purpose for our lives. His work for you may not look or feel like anything you had in mind, but know this, He will complete His work in you dear one.


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If you were Jason Bourne, you'd be dead right now. Part 1 of 2.
I want a smaller house, and one near the airport. A great home to cycle and run and garden and be outside.
I'm building in one of the biggest planned communities in the U.S., Daybreak in South Jordan. It has outdoor pools, organic community gardens, many miles of trails, a river, several sandy beaches, and volleyball, soccer, and tennis facilities within two blocks of my house.
A huge amount of effort was involved, to get a decade of accumulated stuff out of here. Given away, put in storage. All the evidence of my kids' childhoods, mostly gone. I kept only a few boxes of favorite books and dress-ups I'm saving for my children's children one day.
My house looks so shiny and new right now I kinda want to change my mind, and stay. Landscaping, windows, closets, looking perfect, like no one lives here.
So I have two challenges right now. ONE, keeping this house immaculate. Like, Home Show immaculate. While my children and puppy live here. While I'm out of town sometimes.
I said to them:
"You are Jason Bourne! When you walk in, you make sure there are no traces of you. Assume the police or the bad guys will be here in 10 minutes. You live to see another day only if it looks like you were NEVER HERE.
"You drink a glass of water, or eat something? Not only do you wash the dish and put it in the dishwasher, but you wash and polish the sink, too!
"There's no Bourne Supremacy, Bourne Ultimatum, let alone Bourne Legacy, if you fail in this mission. You're just dead."
(It's rather pathetic how much I know about 10 years of Jason Bourne movies. I draw on what I know! I'm fighting the urge to go sideways right now about how I feel about Matt Damon's non-participation in the fourth movie.)
It's not going that well. It isn't that my kids aren't trying to be neat and clean. They actually are.
(The threat hanging over their head goes like this: "A realtor finds dried toothpaste in the sink? Your shoes lying around here? You have to go stay at your dad's. Zero tolerance, pal.")
The problem isn't motivation, or how hard they're trying. It's that they have a life-long history of being habitual slobs.
Lately I've been trying not to bark at them, by saying instead, something really kind and encouraging:
"You suck at being Jason Bourne."
They've been saying the same thing to me. Turns out I leave empty glasses and shoes around here, too.
My second challenge, as my family faces this major transition, in my next post.
Posted in: Relationships
One thought on "If you were Jason Bourne, you'd be dead right now. Part 1 of 2."Leave a Comment
Haha! This post just made me laugh, because we are in the same boat right now. We are selling our home, and I have 4 children ages 8, 6, 2, and 4 months. So . . . pretty much impossible to keep it immaculate. We traveled up to stay with the grandparents just so it would stay clean (might have driven Grandma a little nuts, though!) I must say, it worked, because we got an offer. And I stayed sane!


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Splout SQL
Technical details
Getting started
Installing & running Splout
Loading an example dataset
User guide
Download as PDF
Source code
Developers group
Issues system
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Users group
Building Splout
Splout SQL F.A.Q.
What's the difference between Splout SQL and Dremel-like solutions such as BigQuery, Impala or Apache Drill?
Splout SQL is not a "fast analytics" Dremel-like engine. It is more thought to be used for serving datasets under web / mobile high-throughput, many lookups, low-latency applications. Splout SQL is more like a NoSQL database in the sense that it has been thought for answering queries under sub-second latencies. It has been thought for performing queries that impact a very small subset of the data, not queries that analyze the whole dataset at once.
Is it really so fast?
Splout SQL is as fast as SQLite can be. The very good thing about Splout SQL is that, because it is a read-only store and data is replaced entirely every time, it always has the data optimally indexed, there is zero fragmentation and data colocation in disk can be controlled using sorting in the Hadoop indexer process (insertionOrderBy). As an example, we have used data colocation techniques within Splout SQL to obtain < 50ms average query time with 10 threads on dynamic GROUP BY's that hit an average of 2000 records each in a multi-gigabyte database that exceeded available RAM in orders of magnitude in a m1.small EC2 machine.
Can I import data directly from Hive into Splout SQL?
Yes, since release 0.2.2 it is possible to integrate Hive directly with Splout SQL. It is also possible to do the same with Cascading or Pig. Please read the
user guide, section Integration with other tools
I am experiencing slow queries, why?
Splout SQL is optimized for indexing data according to custom needs. You can create arbitrary indexes and colocate data at insertion time for minimizing disk seeks. Please read the
Troubleshooting section of the user guide
Can a DNode use more than one disk for storing data?
Currently a DNode's working directory is fixed to be in a single disk location, however, there is nothing that prevents you from installing two DNode services in the same machine, as long as you configure everything properly so that they bind to different ports, for example.
Can I execute INSERTs / UPDATEs on Splout SQL?
There is nothing that will block or prevent you from executing INSERT / UPDATE statements through Splout SQL's interface. However, it is not the way you are supposed to use it. Splout SQL has been conceived to be a read-only store, and its data can be updated entirely by an atomic deploy mechanism, meaning that the whole dataset is replaced by another version of it. This fits well into batch-processing since usually you will have a new copy of the whole dataset each time you run your batch process, for example in Hadoop. But it doesn't fit well if you want to incrementally update your dataset in real-time.
But really, what happens if I INSERT / UPDATE?
What will happen is that your statements will be executed in one of the replicas of the partition that was hit, so you will have unconsistent data across partitions. It would be only fine if you didn't use replication, but in that case you wouldn't have failover, so it's not something you really want to get into.


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Monica and Devon Jones' parenting philosophy has always been about giving their children the freedom to grow. But teaching their now 5-year-old daughter life lessons almost cost them their custody.
Last year, the couple allowed their then-4-and-a-half-year-old daughter to play at the park across the street while one of the parents watched from a distance.
Someone spotted the child playing alone and called Child Protective Services to investigate.
"A few weeks later CPS came to our house and interviewed us. They wanted to find out what was going on and then proceeded to tell us they were going to find substantiated neglect," Devon Jones said.
The couple appealed the findings and were prepping to fight the charges in court, but inconsistencies and a missing witness led to the charges being dropped.
The couple is now calling for a change. They are in the process of gathering support and created the Facebook page Free Range Kids Colorado in hopes of bringing a "free-range" parenting law to Colorado. The law was just passed in Utah, making it the first state to let parents give their kids more freedom to be on their own without fearing they'll be charged with a crime. The child can't be in imminent danger of being harmed.
"It gives it another level of protection to parents who just want to let their kids play outside. Since it's discretionary we have no actual guidelines as to when it's safe to allow our daughter to go out and play with other kids," Devon Jones said.
The new law in Utah passed without a single "no" vote.
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While other lists may be recommending books that came out in the last month, I will only recommend books that I have cooked from.
Momofuku was published in 2009, and I received it as a Christmas gift from Mrs. Dude. Momofuku is a book that's hard to classify - and that is its biggest strength. This is a book eschews words like "authentic" and strives instead for "tasty". When he can't get the right kind of kelp in New York, he substitutes high quality bacon. We keep around his ramen broth in the freezer, and serve it up on weeknights with some veggies and noodles. It's my favourite thing to eat on a weeknight. His rice cakes were unique and delicious.
While I wouldn't say that the recipes in this book are challenging, they do require commitment. The ramen broth is not challenging to make, but it does basically take a full day. And many of the recipes later in the book require things created in earlier recipes in the book.
Seven Fires is an awesome Argentine barbecue book. This is the perfect book for the barbecue enthusiast in your life. Most of the recipes are relatively simple with few ingredients, and bright, fresh flavours. Take the Argentine steak with chimichurri. Nothing could be simpler to prepare on the grill (Cooking steak is easy. Cooking steak perfectly takes practice - so practice a steak tonight!). Or the grilled chapa bread. Start a couple hours in advance, and you can serve it with your dinner. The flavours here are simple, but really great. They emphasize fresh herbs and acids to brighten them. I can't recommend this book enough.
A newer one, Meat: A Kitchen Education was published in October of this year. For such a slim volume, it's really a very complete reference on all things meat, and has recipes for all kinds of cuts. A recent favourite was the beef shank. This book also includes sections on game, and less-common meats (at least in this part of the world) like squab. It's a more ambitious book than Seven Fires, but an excellent addition to any cook's library for simple information like learning how to dismember poultry.
In the "late-night reading" category, the biography of Ferran Adria, Ferran is a good read.
This book takes you from the simple beginnings of the experimental restaurant El Bulli, which started as a pretty sleepy little restaurant in the middle of nowhere that often struggled to have a single visitor in a night, to becoming the hardest-to-get restaurant reservation in the world. (Check out some well-photographed food here). The author is no sycophant, indeed proclaiming that there are dishes he's been served at El Bulli that are "vaguely punitive" or take this quote:
Just your everyday mix of sea anemone, raw rabbit brains, oysters and calamondin (a sour-sweet Southeast Asian citrus) in lukewarm dill broth. I wouldn't go as far as one blog entry I later happened across, which described this creation as "Vile. Vomitous. Nightmare!" But I found it so utterly unpleasant and cacophonous that I wondered for a moment whether Ferran had gone off the rails. It made my teeth ache.
This is a fun read. Even for those of us who will never get to eat at El Bulli, Ferran Adria has affected cooking in ways that we'll be feeling for decades. Fascinating read.
And the final food book that I'm recommending is Catching Fire: How Cooking Made Us Human.
Okay, so this one I will not unreservedly recommend for the foodie in your life. I loved this book. But, you know. I'm a microbial geneticist. I love human genetics. I love hearing about the evolution of early hominids. This book describes how much of an advantage cooking was to early humans, and how much it has shaped our evolution. All animals extract 20-30% more calories out of cooked food. We require *much* less time to chew cooked food, freeing up time to hunt, work and write poetry. (Indeed, it takes so long to chew raw meat, that most meat-eating non-human primates will only eat the intestines and organs of other animals - the meat takes too long to chew).
If you're interested in cooking, and you find human evolution interesting, you'll love this book.
My final Christmas present recommendation is a bit of a surprising one (and a bit of an expensive one). Get a smart phone. I got a Droid this year.
Motorola DROID II is an excellent phone. I use it almost every week in the kitchen. I take my recipes with me to the homes of my friends and my family. And I look up tidbits in the kitchen. I knew that I would find my smartphone useful. I had no idea I would use it in the kitchen as often as I do.
And finally, to me, the best parts of Christmas are about food and family. Spending the day cooking, eating, talking and singing. Watching my son play, and giggle. In the end, the gifts don't really matter. Don't stress. Make something delicious and share it with your family.
Merry Christmas, and Happy Holidays.


text:
About Us
Learn a bit about CBD Health of Florida
Company info
We are professional CBD brick and mortar stores located in Pinellas Park Florida. That's the Tampa- Clearwater area. We offer a full line of quality CBD products manufactured in the U.S.. We have a knowledgeable staff set in a professional atmosphere.
got my life back
I opened these stores because of what CBD has done for me. I was in a car wreck 2 ½ years ago. I injured my neck, arms, and hands. I have 2 herniated discs and 3 bulging discs in my neck and torn ligaments in my shoulder and hands. I went through pain management doctors, surgeons and physical therapists. I had procedures done without getting any relief. For 2 years I was told to get used to the pain and take the prescription drugs they gave me. The worst 2 years of my life. In February of 2018 a friend of mine sent me a bottle of CBD tincture to try. I couldn't believe what happened next. I felt great again and had my life back. So, I decided to invest in a store to help others. I hope we can help you.
John. M -Founder and Partner


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This week, we'd like to make our readers aware that some third party sellers on Amazon have been selling fake copies of Rory's Story Cubes. They come with blank plastic "dice" and icon stickers to attach to them. We here at Gamewright are doing our best to monitor and shut down the perpetrators.
The original Rory's Story Cubes and the brand new Rory's Story Cubes – Actions both come in a sturdy box with all icons embedded on the cubes, no separate stickers included. While imitation is a form of flattery and this surely is a result of the massive sales of the game over the last couple of years, we don't want any of our customers to pay for a lower-quality version of this game than the one that our team has worked very hard on creating, promoting and selling.
If you happened to receive a copy of the impostor cubes, please contact firstname.lastname@example.com with the name and contact information of the seller, so that we can hopefully track them down and ask them to cease selling.


text: EGGER Aqua+ laminate flooring has outstanding water-resistant qualities and suit therefore perfectly for installation in bathrooms. With the click system UNIfit! is the floor fast and easily installed, as you can see in this film. The easy care, long life and durability make these floors perfect for all living areas, as well as many commercial applications.


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The Heidelberg Catechism
The Heidelberg Catechism was written in Heidelberg at the request of Elector Frederick III, ruler of the most influential German province, the Palatinate, from 1559 to 1576. This pious Christian prince commissioned Zacharius Ursinus, 28 years of age and professor of theology at the Heidelberg University, and Caspar Olevianus, 26 years old and Frederick's court preacher, to prepare a catechism for instructing the youth and for guiding pastors and teachers.
Frederick obtained the advice and cooperation of the entire theological faculty in the preparation of the Catechism. The Heidelberg Catechism was adopted by a Synod in Heidelberg and published in German with a preface by Frederick III, dated January 19, 1563. A second and third German edition, each with some small additions, as well as a Latin translation, were published in Heidelberg in the same year. The Catechism was soon divided into fifty-two sections, so that a section of the Catechism could be explained to the churches each Sunday of the year.
In The Netherlands, this Heidelberg Catechism became generally and favorably known almost as soon as it came from the press, mainly through the efforts of Petrus Dathenus, who translated it into the Dutch language and added this translation to his Dutch rendering of the Genevan Psalter, which was published in 1566. In the same year, Peter Gabriel set the example of explaining this catechism to his congregation at Amsterdam in his Sunday afternoon sermons. The National Synods of the sixteenth century adopted it as one of the Three Forms of Unity, requiring office-bearers to subscribe to it and ministers to explain it to the churches. These requirements were strongly emphasized by the great Synod of Dort in 1618-19.
The Heidelberg Catechism has been translated into many languages and is the most influential and the most generally accepted of the several catechisms of Reformation times.
The Catechism is broken into three main themes or parts: Man's Misery, Man's Deliverance, and Man's Gratitude. Other ways of describing these themes are: "Grief, Grace, Gratitude" or "Sin, Salvation, Service."
Introduction: LORD'S DAY 1
Q & A 1: What is your only comfort in life and in death?
That I am not my own,1
but belong--
body and soul,
in life and in death--2
to my faithful Savior Jesus Christ.3
He has fully paid for all my sins with his precious blood,4
and has set me free from the tyranny of the devil.5
He also watches over me in such a way6
that not a hair can fall from my head
without the will of my Father in heaven:7
in fact, all things must work together for my salvation.8
Because I belong to him,
Christ, by his Holy Spirit,
assures me of eternal life9
and makes me wholeheartedly willing and ready
from now on to live for him.10
11 Cor. 6:19-20
2 Rom. 14:7-9
3 1 Cor. 3:23; Titus 2:14
4 1 Pet. 1:18-19; 1 John 1:7-9; 2:2
5 John 8:34-36; Heb. 2:14-15; 1 John 3:1-11
6 John 6:39-40; 10:27-30; 2 Thess. 3:3; 1 Pet. 1:5
7 Matt. 10:29-31; Luke 21:16-18
8 Rom. 8:28
9 Rom. 8:15-16; 2 Cor. 1:21-22; 5:5; Eph. 1:13-14
10 Rom. 8:1-17
Q&A 2: What must you know to live and die in the joy of this comfort?
Three things:
first, how great my sin and misery are;1
second, how I am set free from all my sins and misery;2
third, how I am to thank God for such deliverance.3
1 Rom. 3:9-10; 1 John 1:10
2 John 17:3; Acts 4:12; 10:43
3 Matt. 5:16; Rom. 6:13; Eph. 5:8-10; 2 Tim. 2:15; 1 Pet. 2:9-10


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Eddy, Noah and I made it home tonight from our wonderful week in Dorset with my family. As I said in my previous blog, it has been a lovely week with the family, chilling out in the most beautiful surroundings with some of the most beautiful people!
We really have had a great week's break and, as we sleepily made it through our front door and tucked little Noah into bed, I took one look at the mountain of luggage and thought to myself...tomorrow!
Normally I would wait up and unpack everything, but I think we are all a little bit too exhausted and so, apart from this mini blog post, I think we are going to chill out and get a nice early (ish) night in!
I wanted to mention one thing that has made my packing and organisation really easy this week, that has been a new roll up cosmetic/wash bag. Predictably, this is another Cath Kidston piece, but I must admit I did pick it up in a recent sale! My sisters and my mum all have different CK wash bags and I think it's really cute when we all go somewhere together that we get our matching bathroom bags out. They're just so girly and fun! I know these have been around for a long time, but I was pretty excited about mine and thought it deserved a little mention...
What I love about it is that it has neat little compartments so that you can divide your items up so they are not just a heap of confused lotions and potions when you come to use them. I have divided mine into a family teeth brushing section, a face section, a hair section and then, at the bottom there is a handy zipped pocket for additional items. The whole thing rolls up into a neat and compact travel bag and, one you arrive at your destination, the handy hook at the top allows you to hang it up anywhere. This will be ideal for our camping holiday next week as I could hang it in our tent and know exactly where to reach for what I want! Perfect!


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Dénicheuse - treasure hunting
It's the treasure hunter's passion to track down a thousand and one little things that take us beyond the everyday, or transform the everyday into something more beautiful.
At dénicheuse.com, you will find a very personal selection of objects – unusual, beautiful, practical or a bit offbeat, designed to make life a little more cheerful, more sparkling and more colourful: things that will make you smile. Creations of young designers, objects from small start-up companies, or pieces selected from more well-known brands: at denicheuse.com you will find things that can't be found just anywhere.
These are treasures that I have gathered in the course of an incessant search for the kind of objects that I love. And I want to share them with you.
Treasure hunting as a profession
Dénicheuse.com is a very personal adventure. A few months ago, after 10 years in charge of communications at Mudam Luxembourg, I decided to turn my passion into my profession.
And to awaken in others their own lust for discovery.
These objects, these jewels, these garments all have a story, the story of their creators scattered around the world, the designers and the brands they have created. Whether single, in a couple, as childhood friends or mothers, they were always impassioned, always with the courage to follow their ideas through, and to imagine, create and construct extraordinary things.
I have kept in personal contact with every one of these creators whose story has so touched me.
In turn, they have all been touched by my adventure and have encouraged me on my journey. It is thanks to their confidence and their unfailing support that I have had the courage to launch myself on this amazing adventure.
What can you find on dénicheuse.com ?
Playful, original, funny, quirky, but always affordable, these objects are part of the art of making daily life less ordinary.
They link the useful to the lovely, they make people smile, they are always surprising; in short, they are an antidote to the drudgery and boredom of daily life.
I select all the products with immense care, but above all with love and passion.
These creations have seduced me with their beauty, their originality, their poetry, their mellow clarity, their quality – often all at the same time. They are objects that you can buy for your own delight, or to bring pleasure to someone you love.
Why an online shop?
The selection in my online shop is very personal and will grow from day to day in the course of my discoveries.
One of its charms is that it is open 24/7.
You can browse the "shelves" and let yourself be seduced by the little things on offer, hunt for bargains at your leisure and in your own time.
You like treasure hunting too?
If you also have the heart of a treasure hunter, share your favourites with me!
If I take up your suggestion and choose to present it on my site, I promise to mention your name along with the description of the product.
A little surprise will be sent to you to say thank you for your discovery.
So don't hesitate!


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» Tuesday, March 30, 2010
Prevent Programme
Asked about the Select Committee report, the PMS said that the Prime Minister himself had not expressed a view, but the important thing was for the Government to consider the report and its recommendations and what impact they might have in practice before responding more fully. It was an important report and it needed to be given proper consideration.
Asked if it had been discussed in Cabinet, the PMS said that it had not. Put that the Government had been giving money and a voice to unelected Muslim leaders, the PMS said that at this stage it was important we considered the report and its recommendations and then responded more fully.
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Japan-based Toyo Engineering Corp said on Monday that Khafji and Hout oilfields in fields jointly operated by Saudi Arabia and Kuwait in the Neutral Zone will resume output in 2019.
Toyo said it won a third renewal of general engineering services agreement (GESA) from Al-Khafji Joint Operations (KJO), under which the two nations jointly operate Khafji and Hout Oil Fields.
Following the renewal, Toyo said it is scheduled to support KJO until 2023 on project planning feasibility study, FEED and technical support.
"Due to lower crude oil prices, the production of the oil fields was suspended in 2014. Because of oil price recovery, KJO starts the preparation work to re-produce the oil from the fields from 2019," the Japanese firm said in a statement.
The maximum oil production rate of the fields is 350,000 barrels per day, it added.
Khafji and Hout oilfields were discovered and developed by Japanese Arabian Oil Company Ltd. (AOC) in 1960. From 1980, KJO has been established among AOC, Saudi Aramco and Kuwait Petroleum Company, the firm said.
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ARIES (March 21-April 19) HHHH You might want to clarify what is happening financially with a partner or close friend. You could feel stressed out by what you see and/or hear, but you will be able to move forward because of a general sense of well-being. Others appreciate your actions. Tonight: Your treat.
TAURUS (April 20-May 20) HHHHH The summer often carries a lot of tension for you. Right now, you could feel forced in a certain direction. Your friends or someone close to you will prove to be a very positive force. This person supports you in your ventures and your attitude about living. Tonight: As you like it.
GEMINI (May 21-June 20) HHH You have a lot on your mind, yet you might not be sure of your choices. A wise investment could come through for you. A partner won't be supportive of your needs. In fact, this person might toss what you ask for right back in your face. Tonight: Choose your company with care.
CANCER (June 21-July 22) HHHH You are more upbeat than you have been in a while. During a meeting, you'll wonder why everyone is being so positive. You see a situation in a different light than many. It would be wise to share your vision and explain your rationale step by step. Tonight: Where people are.
LEO (July 23-Aug. 22) HHH Take a stand, if you must. You could feel pressured by a commitment involving business or your community. You also might feel burdened by domestic matters that require your full-time attention. Trust that your instincts will be right on. Follow them. Tonight: Till the wee hours.
VIRGO (Aug. 23-Sept. 22) HHHH You'll want to understand all aspects of an issue, as well as what the different parties involved think about it. A meeting will prove enlightening, and it could help direct you toward the appropriate path. One person could be defensive and difficult. Tonight: Be impulsive.
LIBRA (Sept. 23-Oct. 22) HHH You might not be sure how to dissolve a group discussion of a topic effectively. Count on your resourcefulness; you will come up with many solutions after the fact. Do not push a partner too hard about an invitation involving the two of you. Tonight: Let off steam by taking a walk.
SCORPIO (Oct. 23-Nov. 21) HHHH Someone feels challenged by you, so be ready to take a stand. It is not an issue of the quality of your work or ideas; rather, this person feeds off making you look foolish. Don't worry - others see through his or her game. Tonight: Say "yes," and go along with the program.
SAGITTARIUS (Nov. 22-Dec. 21) HHH You will be taken aback by someone's good fortune, which also could impact you. Understand what is expected, and deliver just that. Luck will come to you, as long as you don't become sarcastic. If you do, you won't believe what you could trigger. Tonight: Off to the gym.
CAPRICORN (Dec. 22-Jan. 19) HHHH You might be juggling a lot, but you can get through it. Tap into your creativity in order to eliminate what is no longer necessary. Your ingenuity could save the day. Be careful with someone whose temper could trigger you over nothing. Tonight: Catch up with a loved one.
AQUARIUS (Jan. 20-Feb. 18) HHH You will find it intuitive and natural to stay close to home. If you have a home-based business, it will be easier. Be careful with electronic devices and machinery, as your mind might be elsewhere, and you could have an accident. Tonight: Adapt to someone else's mood.
PISCES (Feb. 19-March 20) HHHH You have a lot on your mind. It would be wise to start sharing your thoughts with others, especially when they are relevant to them. You will feel less pressured as a result. A child could be unusually difficult, and a new flirtation might be touchy. Tonight: Pretend it is Friday night.


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Additional Resources
White or square-lipped rhinoceros, African Congo.
Akeley Memorial Hall of African Mammals.
35mm color slide, #108
AMNH Library - Special Collections
Carroll, Dr. Richard W.
Forest elephant in bai.
Dzangha-Sangha, Dec. 1, 1992.
World Wildlife Foundation.
Lang, Herbert (1879-1957)
The post of Medje
[with view of Langs
large format camera and tripod].
April, 1914(?).
Glass stereoscopic view. 7 x 13 cm.
ca. 1903.
Picture Collection, The Branch Libraries, The
New York Public Library
Okapi group, rain forest of African Congo.
35mm color slide, #1709
Silver gelatin copy print of postage stamp from
Belgian Congo, 1953.
Great Fish Owl, from live specimen.
Avakubi, Congo Belge. Aug. 21, 1914.
Glass plate negative, 4 x 5 in.
AMNH Library - Special Collections
James Chapin and Herbert Lang, unknown
Photographer unknown.
[James Chapin on S.S. Leopoldville.]
June 1909.
Commercial logging, road clearing.
July 1, 1985.
Pheidole species.
Lantern slide.
Museum of Comparative Zoology
Harvard University
[Herbert Lang]
Lantern slide from negative #104894.
[Lang holding lion cub.]
[Lang, Chapin and unidentified companion at Rehfuhs.]
Babeyru. 1914.
Lantern slide from negative #227163.
Habitat of the Okapi - a brook which some Okapis
often traversed.
Niapu, Congo Belge. Jan. 1914.
Glass plate negative, 5 x 7 in.
Chapin, James (1889-1964)
Hypsignathus monstrosus.
Avakubi. Mar 06, 1914.
AMNH Department of Ornithology
Habitat of the Okapi- one of the larger forest
brooks at the height of the rainy (season).
Niapu, Congo Belge. Nov. 8, 1913.
Okapia johnstoni. Okapi male. Total side view,
with Abawa- an Avingura [Avungara], and an excellent hunter.
Akenge, Congo Belge Oct. 15, 1913.
Okondo, King of Mangbetu.
Okondo's Village, Congo Belge. Dec. 1910.
The Okapi; The Newly discovered beast living in
central Africa. By Sir Harry H. Johnston, K.C.B. Plate I, page
660: The Okapi (Okapia Johnstoni) reproduced from the Proceedings
of the Zoological Society, London, 1901, Vol. 2.
Annual Report of the Board of Regents of the Smithsonian
Washington, Government Printing Office. 1902.
Index, col. illus. 782pp.
Some of the secret society of the "Amjota",
(Leopards), before the judge, (Altdorger). At
the left are the 4 delinquents, who are believed
to have killed several people. At the right, the chief of the
village stands, with his interpreter. At the left of the table,
is the court interpreter. Behind the table is Judge Altdorfer.
Avakubi, Congo Belge. Oct. 29, 1909.
The Roosevelt party outside tent.
Africa. Oct. 1921.
Silver gelatin photograph #243385.
AMNH Library - Special Collections.
The caravan of the Congo Expedition. In the centre
of the picture are 15 of the native assistants - 140 men in all.
Pawa, Congo Belge. Oct. 21, 1910.
Airplane view of Leopoldville.
Nov. 1931.
Silver gelatin photograph #275965.
A caravan with tusks of Elephants from the Haut
Ituri.. There are 97 tusks in the caravan.
Avakubi, Congo Belge. Nov. 17, 1909.
[Portrait of Herbert Lang.] ca. 1908.
Toned silver gelatin photograph.
Panthera pardus. Leopard, male, shot by a Pygmy,
with an arrow in the heart. The two men are Pygmies.
Medje, Congo Belge, Gamangui. Feb. 6, 1910.
[Portrait of James Chapin.] ca. 1908.
Wounded Rhinoceros, young male.
Near Vankerckhovenville, Congo Belge. [Apr. 1912?]
[Chapin painting a watercolor of lizard, with
detail of lizard.]
Habitat of Rhinoceros simus - accessories for
group. Clearing made for our camp, when
collecting accessories. Some of loads in foreground.
Near Faradje, Congo Belge. Jan. 1912.
Azande porter with loads. The skin is put on for
protection against the high grass.
Faradje, Congo Belge. Sept.1912.
Pan troglodytes. Chimpanzee, male. Head.
Niapu, Congo Belge. Nov. 12, 1913.
White or Square-mouthed Rhinoceros (Ceratotherium
simm cottoni)
Akeley Hall of African Mammals
Logan, Rota, May, 1950.
Silver gelatin photograph #123650.
Atelerix albiventris. Family of hedgehogs, unrolled.
Faradje, Congo Belge. Mar. 22, 1911.
Okapia johnstoni [Okapi]
Taken by: Charles Coles and Thane Bierwert
June 1938
AMNH Library Special Collections
Army Ants, showing the enormous masses that cover
the larvae between the leaves of a
Pinapple(sic) plant and grass.
Avakubi, Congo Belge. Sept. 30, 1909.
Pan troglodytes. Chimpanzee, female. Portrait
3/4 view.
Faradje, Conge Belge. Apr. 3, 1911.
Young Kingfishers.
Faradje, Congo Belge . Mar. 31, 1911.
Leopold II, King of the Belgians.
Halftone from a photograph by Russell and Sons,
Large White-bellied Swift.
Faradje, Congo Belge. Dec. 24, 1912.
Machaerhamphus alcinus anderssoni.
Avakubi, Oct. 6, 1913.
Birds 7
Avakubi, April 11, 1914
Congo Expedition Field Notebook
Guttera plumifera schubotzi.
Medje, Sept. 7, 1910.
Mabutu man, playing a "Engombi", a musical
instrument. Played by hitting the string
with stiff fiber. The thumb of the left hand touches
the strings, and thus changes the sounds.
Medje, Congo Belge. Apr. 1914.
Campethera punctuligera balia.
Faradje, Oct. 18, 1911.
Tribe Medje. A sort of rasping instrument, "Egnoro",
(to) propitiate the bad spirits of
the forest, and to insure success in hunting,
during the dances especially performed to insure success.
Medje, Congo Belge . Apr. 1914.
Unidentified fish.
Location, date unknown.
Above Coquilhatville, Congo Belge.
July 21, 1909.
Gnathonemus rhynchophorus.
Avakubi, Dec. 23, 1913.
Young Tigrornis, male. From a live specimen.
Niapu, Congo Belge. Dec. 7, 1913.
[The post of Medje.]
April 1914.
Glass stereoscopic negative, 7 x 13 cm.
The porters emerging from the dense forest.
Boyulu, Congo Belge. Sept. 22, 1909
Mangbetu, hut of Kaphiashalks Niapus village.
Near Rungu. June 1913.
Dzangha-Sangha, June 1, 1994.
Designs of Mangbetu houses.
Niangara. Dec. 1910.
Print from stereoscopic negative, 7 x 13 cm.
Ophiocephallus obscuras
Mastacembellus congius
Polypterus ornatirainnis.
AMNH Department of Ornithology.
Old man working on a large band.
Niangara(?). n.d.
[Lang, Herbert] (1879-1957)
Silver gelatin photograph
Medje Mangbetu , decoration of a hut.
North Nala. Oct. 1910.
Lang, Herbert (1879-1957)
[Gracery?] with native drawings representing a
white man
eating with a soldier on guard.
Near Dungu (west). Jan 21, 1910.
[James Chapin.]
Abarambo dance Mansali only women.
Poko. Aug. 1913.
Personal Diaries
May 8, 1909 July 17, 1909
Congo Expedition Notebook
AMNH Library - Special Collections
Personal Diaries
Mangbetu Knives of Iron
Kay C. Lenskjold
Silver gelatin photograph #36872
Black-collared Love bird
Rota, 1963.
Silver gelatin photograph #329517.
version 2.0--Last Updated January 2003 | About
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text:
29 November 2018: Mark Chennells reviews the year's key cases for SCL North East23rd Oct 2018
Event date
Event time
5:45 pm
Event Address
Ramside Hall Hotel, Beaumont Room, Durham, County Durham DH1 1TD
Mark Chennells will present to the Society of Construction Law North East on Thursday, 29 November 2018 in County Durham.
Mark Chennells will speak on "The Year in review: key cases" and Gary McGifford (GT Fairway) will speak on "Is there method in the madness: a presentation of Delay Claims from a Delay Expert's Perspective". The event will be chaired by Simon Lewis (Womble Bond Dickinson (UK) LLP).


text:
Jessica Alba is a coveted name for every fashion brand even though she isn't a fashion icon for many. Her sassy avatar and dazzling looks perfectly compliment her curvy body and flawless skin. The sexy lasso loves to play with her hair and flaunts a new hairdo now and then. Take a peek into Jessica Alba hairstyles.
1. Princess Bun
Jessica Alba's glossy hair is undoubtedly priceless. This picture shows Jessica Alba wearing a princess bun, a classy retro style always keeps up with the trend. This universal hairstyle looks great on Alba. Nothing much, just use of band and ribbon and a few handy tricks you can achieve this edgy look.