text:
Space Exploration 2007
Brian Harvey
Space Exploration 2007 - an exciting new annual for all space enthusiasts everywhere
2005 satellite and rocket launches
satellite recovery news
emerging trends in space exploration
major feats of 2005
data on all launchers currently in operation
schedules of upcoming missions
anniversaries and landmarks
lavishly illustrated
In addition, Space Exploration 2007 presents unique contributions from leading figures in astronomy and space exploration. This year read about building the International Space Station, results of the exploration of Mars, intercepting comets and asteroids, the latest data from space missions to Jupiter's moons, Titan, Saturn and Venus – and lots more.
Read all about it in Space Exploration 2007 now!
Table of Contents
Preface - Welcome to Space Exploration, 2007
Publisher's note
The epic journey begins
The annual review
by Brian Harvey
Building the International Space Station
A site still under construction
by Neville Kidger
Arrival at the red planet
Mars exploration results
by David Harland
Solar system log
4.1 Looking at Mercury
The MESSENGER mission to Mercury
by Sean Solomon and Ralph McNutt
4.2 Visiting Venus
Exploration results from Venus
by David Harland
4.3 Europa's ice next
Exploring Jupiter's icy moons
by Richard Greenberg
4.4 Exploring the ringed planet
Cassini at Saturn
by David Harland
4.5 Secrets of a cloudy moon
Titan: Cassini-Huygens reveals a new world
by Rosaly Lopes
4.6 Catching fragments of our past
Intercepting comets and asteroids
by John Mason
5.1 A Vision for Space Exploration
Rolling out the Bush plan
by John Catchpole
5.2 ...Continuing a Vision for Space Exploration
Stepping stones to Mars - a new strategy
by James Oberg
Soyuz in the jungle
Russia builds a launch base with Europe in South America
by Laurent de Angelis
Return to the Moon
Chang'e and Chandrayan lead the way
by Paolo Ulivi
In the footsteps of Soyuz
Russia's Kliper spacecraft
by Bart Hendricx
192 pages; colour throughout
Publication Date:
September 2006
Praxis Publishing - Leaders in Scientific Publishing © 2008 All rights reserved


text:
macrumors member
Original poster
- Jul 21, 2007
- 31
- 0
Hello, this is my first post as I'm getting ready to get rid of my Dell space hog and get a macbook. I'm going to start to get accessories for it now, I'm looking into getting a silicone keyboard cover for e-bay (yes I'm scared of the keyboard b/c I was always able to just attach a new one if anything spilled w/ my desktop) so does anyone actually use them or would it just be a clunky thing I will throw out after a week? Also does anyone use the hard snap-on case the fully protects exterior of the macbook? I heard those can scratch the macbook more when you try to remove it and I have a feeling it will look ridiculous lol. I know when it comes to electronics I buy all these protective things and then after the novelty wears off I get rid of the protection stuff (such as cell phone cases etc.) Also, please let me know of any other accessories that would benefit me. I'm definitely going to have more questions later as I really can't wait! Thanks!

subset: commoncrawl


text:
After seasonal feasts, it not uncommon to crave more cleansing and nourishing dishes such as kitchari (or kitcheree or khichdi). Essentially a one-pot Indian meal, the common feature of a kitchari is a digestive aiding combination of split legumes, rice and spices and often vegetables. Such preparations are open to …Read More »
Home / Rice
Guacamole Rice
Avocados are something I usually have on hand. They keep well if purchased when firm and they are simply wonderful just sliced and served up all on their own, just as is for a snack or as part of a meal as a nourishing and refreshing side. They also happen …Read More »


text:
After I finish updating the coaches and stuffs for NBA 2KZ (formely known as NBA & NBAGLeague 2k) trades and transactions in the upcoming season will be very minimal so I'm thinking of making some kind of NBA2k League in NBA 2k14 (of course it is not online game) but in a different way. Hopefully it happened and continued.
Ideas and influence(s): 2K Sports' NBA2k League
Other Ideas and influence(s): ICBA2k League live stream and Ciudad2k League live stream.
* Players: Players consists of PCFs.
* MyCareer, Association and Season compatible:
* Team: Consists of up to 30 unique (fictional) teams. (although I can start the league even with only 5 teams) . minimum of 10 and maximum of 12 players per team. Maybe I will include 1 NBA superstar player per team as an enforcer (import).
* Unique rules and regulations: It must be different from NBA, maybe 7 fouls or something like that so players could play more, etc.
* Team's draft rounds and player's Draft picks will be determined by a computerized "roulette". (Same as TroyDan Gaming is using).
* Windy City Assasin Gaming's "2K Rucker League": "Windy City Assasin Gaming" has full access on NBA2KZ and 2KZ-League based on ModManZZZ's permission so some teams (or all teams) might be seen in 2K Rucker League.
* It will be released: This league will be released so that you can play your team in your computer. WARNING: NOT compatible with ModManZZZ's NBA 2kZ updated roster or any roster files so you have to have another NBA 2k14 main file.
* All-star players based on their performance in the video steaming (I might ask Windy City Assasin Gaming for this) will be included in NBA 2KZ releases in one all-star team.
For those who wants to join in this league: (The start of joining the league will be "ANNOUNCED SOON").
1. Players' registration's requirements:
a. PCF(personal cyberface) is required. Wearing face paint, mask and changing the real name to alias are also considered as PCF and allowed.
b. Height of the players must compute their "real life height in feet and inches" based on 2KZ-league height computation. Formula: 2KZ height = (RealHeightinFeet + 1 & RealHeightinInches - 1)
Example: Real Height is 5'7" = 2KZ Height is 6'6"
c. Weight of the players must compute their "real life weight in pounds" based on 2KZ-league weight computation. Formula: 2KZ weight = Real weight + 90.
Example: Real Weight is 150 lbs = 2KZ Weight is 240 lbs.
d. Players rating and tendencies must be based in real life, if you really a good player in real life (heavy scorer, outdoor league MVP etc.) you can have 80s or 90s rating if you really deserves it so honesty is the key here. You will appreciate your character more if it is really you. Only ONE signatire move is allowed.
e. Players' information (in ros file) must be in Microsoft Excel as shown in the photo.
2. Team registration:
a. Modders and gamers that will join their created teams (any number of teams) has a privilege to add PCFs roster to their corresponding team. If they already have 10-12 players it will never not be included in the draft picks anymore because the roster is already full.
b. Jersey, court and arenas files (*.iff) is required.
c. Logos and dornas files (*.iff) is optional.
d. Any types of courts and arenas are all accepted. (common indoors, streets, parks, and gym).
e. Teams information (also the players' information you added in your team) should be in MS Excel as shown in the photo.
f. Name of arenas/home court is also a requirement in notepad.
I may add additional information.


text:
I'm biased about the Tarantino film because it's a great movie about Los Angeles, the locations are flawless.
I'm biased about the Tarantino film because it's a great movie about Los Angeles, the locations are flawless.
I liked it better than Inglorious Bastards. But then I really didn't like IB to begin with. I found that it added up to much much less than the sum of its parts.
Hollywood is straightforward if you already know the backstory. I watched it with my man, who didn't know the true story, and he also found the first three quarters to be rambling and disconnected, whereas I felt this gradually increasing tension throughout.
My short review is all of the worst of Tarantino and none of the best. I'm usually willing to overlook the ultraviolence and vapidity for that one great scene (case in point would be the first scene in IB) only Tarantino can deliver. I think the dialogue in this one was just drab. My favorite part was the production design of the dog food.
Glimpses do ye seem to see of that mortally intolerable truth; that all deep, earnest thinking is but the intrepid effort of the soul to keep the open independence of her sea; while the wildest winds of heaven and earth conspire to cast her on the treacherous, slavish shore?
Final Destination 2
They got their biggest stupidity out of the way early on. It's right in their setup. Kimberly's premonition happens at an on ramp, just before entering a highway. There are minutes of driving on this highway at 60mph or so before the pileup happens. After the vision ends Kimberly blocks the on ramp. A couple minutes of people being angry and talking to a cop and the truck that starts the whole pileup goes by, and moments later, the pileup ensues. The problem? If she hadn't blocked the on ramp, all of those people would be miles down the road from where the crash that actually happened happened.
Even if you give a hyooj benefit of doubt and choose to believe that was because the triggering event for the pileup to begin was the truck getting ahead of everyone that had been on that on ramp... You have to contend with the fact that no one in the aftermath made that observation. They all took it as gospel that they would have been in a crash that would probably have happened behind them.
There's not much else to say.
Oh, the Mortician coughed up a new piece of lore--that if they brought a new life into the world that wasn't part of death's existing plan, then they could break their curse and no longer be eminently stalked by death. They would no longer be on its most imminent agenda at any rate, presumably because death was going to have to go back to the drawing board. For some reason they went with dying but being revived instead of banging and producing offspring, which is the most logical and correct solution. What could be more of an unplanned life than one conceived people who were supposed to be dead?
But maybe they were concerned about the line they'd danced earlier with their red herring plot about keeping a woman who pregnant at the accident alive until her baby was born. Either angle, even the most correct one, carries a polarizing statement if you have it be the resolution.
"Just because it's 2020 doesn't mean everyone has perfect vision."--catchphrase of a fictional comedian in some movie
russian doll, s1
i watched this cuz i'd heard about how it's an interesting metaphor for trauma (the experience of reliving, processing, recovering) and i really have to fucking agree.
i was so surprised how it picked up starting some time in the middle episodes. RECOMMENDED
i wonder how much flies over the heads of people though who've never been significantly traumatized and/or who've never gone to trauma therapy.
Spoiler: not sure what needs a spoiler tagthe "flashbacks" and handling of different "timelines" was really well done. oh my god, that's really what it feels like sometimes. that moment when nadia first sees her child self, i think i was just as startled and creeped out as her, but i have my own "story" that's a bit like that.
i was totally sucked in and feeling the horror and grief and relief with the characters.
nadia was fucking hilarious as a character
i interpet the very last scene as nadia and alan finding their place in the nighttime traumatized freak parade, surrounded by good company. it made me cry so much cuz that's exactly right(!!!!) and such a great image.
apparently there's gonna be a season 2 but wtf can they do in season 2? it's perfect the way it ends and i'm not convinced that's a good decision. drawing out the metaphor might be really cringeworthy, but whatever, i'll probably watch it.
I finished the first season of the handmaids tale. I don't like zombie apocalypse movies and this was basically a zombie apocalypse movie with political pretensions.
Also, the supermarkets are not how supermarkets would be in a totalitarian regime. I mean, first of all, there wouldn't be supermarkets. Why? 1. Because the handmaids can AND DO congregate in the vast aisles. That's one of the points of them being designed that way in the US, to accommodate for multiple people rather than atomized individuals who zip in and out. 2. Because there aren't any different brands. There's only one type of each thing. So it's not only logical from a social perspective, but also from a material and economic perspective to have smaller stores.
Instead I couldn't stop laughing whenever I saw the supermarket, because they had like 20 rows of identical cereal boxes lined up on a shelf, facing outward as if they were advertising different brands, and 10 identical sausages evenly spaced on a display counter. I mean wtf, whoever designed this should be fired.
Unless they were going for some sort of highbrow art exhibit supermarket? Or spot the non-existent difference games for the future generations of kids? But I don't think the Commanders would like that.
I mean look at this BS
Spoiler: can't resize picture
I like this picture especially because of the last fridge on the right, in which the handmaids apparently decided to take two milk bottles from each side on every shelf, to maintain the perfect symmetry.
I haven't watched it, but wouldn't it make sense that they would adapt the existing stores rather than build new ones? And likewise hold onto some stocking systems/aesthetics from a capitalist system even after the economy had changed?
They think capitalism is evil and there's no functional purpose in spreading out items like that. It's much faster to shop in a smaller place and it saves electric + heating costs (important in a post environmental diaster world)And likewise hold onto some stocking systems/aesthetics from a capitalist system even after the economy had changed?
You've convinced me.
Another reason I never watched the show (aside from how heavy and depressing it seems to be) is that it's supposed to be this allegory for sexism within our current society, but the whole premise is that there's been a major disruption to human reproduction, which is one of the most basic animal drives. Of *course* there would be an extreme reaction.


text:
Discover the Leapfrog solution created for your industry.
Leapfrog's advanced implicit modelling engine, amazing 3D visualisation capabilities and model management products increase your insight and productivity.
Get the maximum from your Leapfrog products with our training and support solutions.
Central offers a flexible subscription model which can scale with your team. There are no limitations of the data you can work with so the you can bring all of your projects into Central.
Central can be purchased with different deployment options to fit your organisations needs. To find out more about Central and the licencing options please fill out the contact for below.
If you are interested in finding out more about Central, please fill out the form below. One of our Central representatives will get in touch with you.
Unfortunately you have Tracking Protection enabled, which means you are unable to display the form at this time. For more information on tracking protection and how to turn this feature off, please refer to Tracking Protection in Private Browsing
You'll shortly be contacted by one of our Leapfrog team members.


text:
New Research In
Physical Sciences
Social Sciences
Featured Portals
Articles by Topic
Biological Sciences
Featured Portals
Articles by Topic
- Agricultural Sciences
- Anthropology
- Applied Biological Sciences
- Biochemistry
- Biophysics and Computational Biology
- Cell Biology
- Developmental Biology
- Ecology
- Environmental Sciences
- Evolution
- Genetics
- Immunology and Inflammation
- Medical Sciences
- Microbiology
- Neuroscience
- Pharmacology
- Physiology
- Plant Biology
- Population Biology
- Psychological and Cognitive Sciences
- Sustainability Science
- Systems Biology
Inducible overexpression of wild-type prion protein in the muscles leads to a primary myopathy in transgenic mice
Edited by Stanley B. Prusiner, University of California, San Francisco, CA, and approved March 6, 2007 (received for review October 6, 2006)
The prion protein (PrP) level in muscle has been reported to be elevated in patients with inclusion-body myositis, polymyositis, dermatomyositis, and neurogenic muscle atrophy, but it is not clear whether the elevated PrP accumulation in the muscles is sufficient to cause muscle diseases. We have generated transgenic mice with muscle-specific expression of PrP under extremely tight regulation by doxycycline, and we have demonstrated that doxycycline-induced overexpression of PrP strictly limited to muscles leads to a myopathy characterized by increased variation of myofiber size, centrally located nuclei, and endomysial fibrosis, in the absence of intracytoplasmic inclusions, rimmed vacuoles, or any evidence of a neurogenic disorder. The PrP-induced myopathy correlates with accumulation of an N-terminal truncated PrP fragment in the muscle, and the muscular PrP displayed consistent mild resistance to protease digestion. Our findings indicate that overexpression of wild-type PrP in skeletal muscles is sufficient to cause a primary myopathy with no signs of peripheral neuropathy, possibly due to accumulation of a cytotoxic truncated form of PrP and/or PrP aggregation.
Diseases affecting the skeletal muscles are fairly common. Many muscle disorders are secondary to pathological conditions affecting the nervous system. Others are related to genetic mutations, immune dysfunction, or exogenous toxins and drugs (1). However, in many primary myopathies, the etiology and pathogenic mechanisms remain unclear (1).
The normal or cellular prion protein (PrPC) is a ubiquitous small glycoprotein attached to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. Although PrP is well established as the central protein in prion diseases or transmissible spongiform encephalopathies (2), its cellular function remains elusive.
PrPC has been reported to be up-regulated in the skeletal muscles of patients with sporadic and hereditary inclusion body myositis (3–4), as well as in patients with polymyositis, dermatomyositis, and neurogenic muscle atrophy (5). Furthermore, transgenic (Tg) mice overexpressing PrPC also developed necrotizing myopathy associated with demyelination of peripheral nerves and vacuolization of the CNS at advanced ages (6). It is not clear whether the elevated level of PrPC in skeletal muscle is a cause or result of myopathy in the affected patients. Although PrPC overexpression is the cause of the muscle disorders in the above-mentioned experiments (6), body-wide PrPC overexpression and the presence of pathology in central and peripheral nervous tissues raise questions about the role of muscular PrPC in the pathogenic process in the muscles.
The present study reports that strictly muscle-specific overexpression of wild-type human PrPC under tight regulation by doxycycline in Tg mice leads to a primary myopathy. These data argue that overexpression of wild-type PrP in the skeletal muscle is in itself sufficient to cause a myopathy. In addition, our data show that the myopathy correlates with preferential accumulation in the skeletal muscles of an N-terminal truncated PrPC fragment, which corresponds to the previously reported C1 fragment (7–11), suggesting that the C1 fragment is involved in the pathogenesis.
Transgenic Mice Exhibiting Doxycycline-Inducible and Skeletal Muscle-Specific Expression of PrPC.
Doxycycline-inducible Tg mice were generated by using the HQK transgene construct with the ORF of two genes: the reverse tetracycline responsive transcription activator (rtTA) under the control of the mouse PrP promoter from the half-genomic vector (12) and human PrPC regulated by the tetracycline-responsive promoter (tetO-hCMV*-1) from the core plasmid (13) (Fig. 1 A). The two genes were in tandem and separated by a spacer sequence. The HQK transgene was introduced into the wild-type FVB mice, and Tg(HQK)/Prnp0/0 mice were obtained through breeding with the Zurich I PrP-null mice (14) in FVB background. Four founders were obtained; line Tg(HQK)18 [referred to as Tg(HQK) for simplicity] was selected for further study.
Skeletal muscles from hind legs (quadriceps), abdominal wall, tongue, brain, spleen, heart, stomach, intestine, thymus, lung, liver, and kidney were collected from Tg(HQK) mice that had been fed food pellets either lacking or containing doxycycline (Dox) for 10 days to induce PrPC expression. No PrP was detectable on Western blots from skeletal muscle and the various organs when Dox was not administered (Fig. 1 B Upper). After Dox treatment, large amounts of PrP accumulated in the skeletal muscles from hind legs, abdominal wall, and tongue, but not in any other organ (Fig. 1 B Lower) except for the heart, where a small amount of PrP was detected after prolonged exposure (data not shown). The muscle-specific, Dox-inducible PrP expression in Tg(HQK) mice was confirmed by RT-PCR analysis, demonstrating the presence of PrP mRNA only in skeletal muscles (Fig. 1 C). The reason for the strictly skeletal muscle-specific PrP expression in the Tg(HQK) mice after induction with Dox is unclear. It appears to be the intrinsic property of the HQK transgene construct because the muscle specificity is observed in all Tg(HQK) lines (data not shown).
PrP expression in the muscles of Tg(HQK) mice was dependent on the dosage of Dox (Fig. 2). After 7 days of treatment with 20, 50, 200, 1,000, or 6,000 mg of Dox per kg of food, muscle PrPC was first detected at a dose of 50 mg of Dox per kg of food, and the PrPC levels increased with higher Dox dosages. Near peak level of induction was achieved with 1 g of Dox per kg of food, but the dosage of 6 g of Dox per kg of food was used to maximize the consistency of induced PrP expression.
Time Courses of PrP Induction and Clearance.
After induction with 6 g of Dox per kg of food, PrPC was first detected in the skeletal muscles at 2 days postinduction (data not shown). The amount of PrP increased with time and reached a near maximum stable level at 14 days postinduction (Fig. 3 A). At the highest level of expression, the PrPC level in the muscles of Tg(HQK) mice was ≈40-fold that of wild-type FVB mice (Fig. 3 A Lower), exemplifying the high inducibility of PrP expression by oral administration of Dox in the Tg(HQK) mice.
The rate of PrPC clearance was assessed by feeding 6 g of Dox per kg of food to Tg(HQK) mice for 7 days followed by administration of regular food lacking Dox, and the PrPC levels in skeletal muscles (quadriceps) collected at 0, 2, 7, and 14 days after Dox withdrawal were examined by Western blot analysis with mAb 8H4 (binding to mouse PrP175–185 and human PrP176–186). PrP expression was found to decrease progressively with time, becoming undetectable at 14 days (Fig. 3 B). Similar results were obtained with muscles from the abdominal body wall (data not shown).
Characterization of PrP in Tg(HQK) Mice.
The PrP in muscles from Tg(HQK) mice treated with 6 g of Dox per kg of food for 7 days or more distributed mostly into a very prominent band of 32 kDa and a much weaker band of 30 kDa on Western blots probed with biotinylated 3F4 (binding to human PrP109–112) (Fig. 3 C). Treatment with PNGase that cleaves off the glycans revealed a strong 27-kDa band and weaker 24- and 20-kDa bands, indicating that the 32- and 30-kDa bands were glycosylated (Fig. 3 C). After reprobing the blot with the mAb 8H4, besides a moderate 32-kDa band and a much weaker 30-kDa band, an additional very strong 27-kDa band and a very weak 24-kDa band were observed (Fig. 3 C); the same band patterns were confirmed when probed directly with 8H4 (Fig. 3 A). After deglycosylation, these bands migrated to 27, 24, and 18 kDa (Fig. 3 C), confirming that most of the PrP expressed in the muscles of Tg(HQK) mice were glycosylated. Similar 32-, 30-, and 27-kDa PrP bands were detected with 8H4 in the skeletal muscles from wild-type FVB mice, but the 32- and 27-kDa bands were of comparable intensity (Fig. 3 A). In the brains of wild-type FVB mice and the Tg40 transgenic mouse line expressing human PrP at wild-type level (15), the 32-, 30-, and 27-kDa PrP bands were also detected with 8H4, but a vast majority was the 32-kDa form (Fig. 3 A).
The prominent 27-kDa PrP fragment in the muscles of Tg(HQK) mice, which shifted to 18 kDa after glycan removal (Fig. 3 C), appears similar to the previously reported C1 fragment (7–11). The C1 fragment is an N-terminus-truncated, glycosylated 27-kDa PrPC fragment with N terminus at residues 111/112 generated during normal PrP metabolism, which also shifts to 18 kDa after glycan removal (9). To evaluate whether the 27-kDa PrP fragment in the muscles of Dox-induced Tg(HQK) mice is actually the same as the C1 fragment, full-length PrP was removed by immunoprecipitation with the mAb 8B4 (against human PrP37–44) (Fig. 3 D). The supernatant contained mostly the 27-kDa PrP fragment and a much smaller amount of 24-kDa PrP fragment; both bands shifted to 18 kDa after PNGase treatment (Fig. 3 D), indicating that they both were glycosylated. Muscle homogenates from Tg(HQK) mice were then compared by Western blot with tissue homogenate from autolysed Tg40 mouse brains. Autolysis of human brain tissue has been shown to convert most full-length PrP to the C1 form (16). Fig. 3 E shows that the deglycosylated 18-kDa PrP fragment from the muscle of Tg(HQK) mice migrated at the same position as the C1 fragment from the autolysed brain of the Tg40 mice. In addition, the 27-kDa fragment and the deglycosylated 18-kDa fragment were not detected by mAb 3F4 (Fig. 3 C), which recognizes human PrP residues 109–112, indicating that these PrP fragments have an N terminus downstream of residue 109. Together these data confirm that the 27-kDa PrP fragment in the muscles of Tg(HQK) mice is indeed the C1 fragment.
To evaluate the protease resistance of PrP in the Tg(HQK) mice, skeletal muscle tissue homogenates from Tg(HQK) or wild-type mice treated with 6 g of Dox per kg of food for 7 days were subjected to digestion with an increasing amount of proteinase K (PK). Fig. 3 F shows that PrP in the Tg(HQK) mice is partially resistant to 5 μg/ml of PK, whereas PrP in the wild-type mice is totally digested at 2 μg/ml of PK. Similar results were obtained for Tg(HQK) and wild-type mice treated with Dox for 2, 4, 14, 30, and 60 days (data not shown). These data indicate that, in the Tg(HQK) mice, muscular PrP is moderately more resistant to PK digestion than that of wild-type mice, and the PK resistance remained constant throughout the course of Dox induction.
Progressive Primary Myopathy in Tg(HQK) Mice with Dox-Induced PrP Expression.
The skeletal muscles from Tg(HQK) mice after increasing duration of Dox treatment were subjected to histological examinations. For Tg(HQK) mice, a few scattered cells with central nuclei in skeletal muscles were visible after as early as 7 days of Dox treatment. After 4–5 weeks of Dox induction, ≤15% of the fibers in muscle fascicles exhibited one or more centrally located nuclei; some showed rare necrotic fibers and regenerating fibers. These changes were accompanied by a mild decrease in fiber diameters affecting both slow- and fast-twitch fibers when compared with those in similarly treated wild-type FVB mice. There was no evidence of mitochondrial proliferation, fiber splitting, or denervation atrophy (e.g., target fibers or fiber type grouping). Acid phosphatase staining revealed a prominent increase of lysosomes within muscle fibers. Minimal endomysial fibrosis was identified (data not shown). These morphological abnormalities became increasingly more pronounced in later stages of the disease (7–29 weeks after induction), with prominent atrophy, degeneration, and regeneration of myofibers accompanied by central nuclei in over one-third of the muscle fibers. In addition, hypertrophic fibers, sparse split fibers (partially or completely split into two by a thin septum extending from the endomysium), and excessive lysosomal activity (acid phosphatase) were seen in virtually all muscle fibers at this stage (Fig. 4 D). Mild mitochondrial proliferation occurred in some of the muscle fibers and in some instances even approximated "ragged-red" fiber formation. Neither inclusions nor amyloid-like materials were detected by H&E (Fig. 4 B) or Congo red stains (data not shown). To examine the effect of prolonged Dox treatment, wild-type FVB mice and PrP-null mice (FVB/Prnp0/0 ) were also subjected to the same Dox treatment and examination, but little pathological change was observed in these mice after treatment with Dox for 200 days (Fig. 4) or >1 year (data not shown). In addition, immunohistochemical staining with 3F4 shows that PrP was distributed throughout the muscle cells, whereas a small number of muscle fibers contained many intensely stained dots (Fig. 4 F), consistent with the presence of PrP aggregates.
Correlation of the Myopathy in Tg(HQK) Mice with Accumulation in Muscles of an N-Terminal-Truncated PrP Fragment.
The amount of the C1 fragment appears to be much more than that of the full-length PrP in the muscle of Tg(HQK) mice (Fig. 3), which is unusual because the level of the C1 fragment was reported to be 30–50% of the full-length PrP in the autopsied brains of normal humans (9). The time course of Dox-induced expression of the full-length PrP and the C1 fragment in the muscle of Tg(HQK) mice was examined more closely after deglycosylation by Western blot with 8H4 and compared with that of similarly treated wild-type FVB mice (Fig. 5). The data show that, in Tg(HQK) mice, both full-length PrP and the C1 fragment increased with time and reached near peak levels at 14 days (Fig. 5 A). The C1 fragment was in submolar amount relative to the full-length PrP up to 4 days, but the C1 fragment was detected at approximately three times the level of the full-length PrP from 7 days and beyond (Fig. 5 A and C). In contrast, the levels of the C1 fragment and the full-length PrP and their ratios remained relatively constant throughout the time course in the Dox-treated wild-type FVB mice, with the C1/full-length ratio fluctuating between 0.98 and 1.25 (Fig. 5 B and C). The difference in the ratios of C1/full-length PrP between the Tg(HQK) and wild-type FVB mice is highly significant (P < 0.001, Student's unpaired t test). This experiment was repeated three times with similar results. These findings indicate that the myopathy is correlated with the high ratio of C1/full-length PrP in the skeletal muscles of Tg(HQK) mice.
We have generated four lines of Tg(HQK) mice where expression of PrP is highly regulated by Dox and stringently limited to the skeletal muscles. After sustained PrP overexpression, these Tg(HQK) mice develop a rapidly progressive primary myopathy. Furthermore, an N-terminus-truncated PrP fragment, which matches the previously described C1 fragment (7–11), is present at three times the level of full-length PrP in the muscle of Dox-induced Tg(HQK) mice.
Transgenic mice overexpressing wild-type PrP in a body-wide fashion have been reported to exhibit myopathy in the advanced age (6), but these mice also overexpress PrP in the nervous system and developed peripheral neuropathy, making it unclear whether the myopathy is primarily due to the accumulation of PrP in the skeletal muscles or it represents neurogenic atrophy secondary to the impairment of the CNS. Furthermore, transgenic mice (named PG14) with whole-body expression of a mutant PrP with nine extra octapeptide repeats were reported to develop a progressive primary myopathy (17). The PG14 mice also developed spontaneous neurological illness, and the mutant PrP in the brain and peripheral tissues (including skeletal muscles) showed mild resistance to protease digestion and other biochemical features reminiscent of the pathogenic form of PrP (PrPSc) found in prion-affected mammals (17–18). In addition, hamsters subjected to daily i.p. injections of chloroquine for 60 days also developed a myopathy that was accompanied by accumulation of PrP in the muscle fibers (19).
Our data demonstrate that overexpression of wild-type PrP in the skeletal muscles alone is sufficient to cause myopathy. These findings strongly suggest that the elevated levels of PrP found in the skeletal muscles of human primary myopathies, such as inclusion body myositis (3–5) and inflammatory myopathies including polymyositis and dermatomyositis (5), may play an important role in the pathogenesis.
The pathogenic mechanism of the PrP-mediated myopathy remains to be determined. The preferential accumulation of the N-terminal-truncated C1 fragment pointed to the C1 fragment. Tg mice expressing N-terminus-truncated forms of PrP following deletion of residues 23–121 or 23–134 develop loss of neurons in the cerebellum (20). Similarly, Doppel (Dpl), a protein with limited sequence homology to the C-terminal part of PrP, is also toxic when expressed in neurons in the absence of wild-type PrP (21), suggesting that Dpl and some C-terminal fragments of PrP are neurotoxic and the toxicity can be neutralized by the presence of sufficient full-length PrP. The toxicity of the experimentally generated N-terminus-truncated PrP has been tentatively explained by the significant inhibition of PrP internalization (22–23), delayed translocation to the cell surface, and prolonged half-life of the truncated PrPC (23).
In contrast to these experimentally generated N-terminus-truncated fragments, the N-terminal-truncated PrP fragment observed in large amounts in the skeletal muscles of Tg(HQK) mice corresponds to the C1 fragment reported to result from normal PrPC processing (7–11). The C1 fragment was also observed at much lower levels in the skeletal muscles of wild-type mice, where the C1/full-length PrP molar ratio was close to 1 (Fig. 5). After synthesis in the endoplasmic reticulum, most of the PrPC is transported to the cell surface and attached by a GPI anchor to the caveolae, specialized plasma membrane microdomains that mediate key processes such as signal transduction and transcytosis (24). Surface PrPC undergoes constant internalization (25), and it is cleaved by the disintegrin family of metalloproteases (26), likely in an endocytic compartment (25), to generate the C1 fragment. The cellular function of the C1 fragment is not known.
In the Dox-induced Tg(HQK) mice, the high levels of the C1 fragment in skeletal muscles imply that production of this fragment was increased and/or its turnover was slowed down in the Tg(HQK) mice. The prominent increase of lysosomal activity in the muscle fibers starting from the early stages of Dox treatment (Fig. 4 D) would provide a mechanism for enhanced production of the C1 fragment.
In Tg(HQK) mice treated with 6 g of Dox per kg of food, the ratio of C1/full-length PrP reached 3.0 after 7 days and it remained relatively constant afterward; initial signs of myopathy also became visible after 7 days, and the disorder progressed rapidly. The correlation of the unusually high C1/full-length PrP ratio with myopathy in the Tg(HQK) mice suggests that the observed muscle cell toxicity is likely mediated by the C1 fragment through a mechanism similar to the neurotoxicity mediated by the experimental N-terminus-truncated PrP forms (20). The C1 fragment observed in normal cultured cells and brain tissues may be tolerated because of its low concentration and coexistence of roughly equal or higher molar amounts of full-length PrPC.
Muscular PrP in the Tg(HQK) mice is moderately more resistant to proteinase K than that of wild-type mice (Fig. 3 F), suggesting low levels of PrP aggregation in the Tg(HQK) mice. However, the significance of this finding is unclear because the PrP PK resistance was constant throughout the course of Dox treatment, whereas signs of myopathy were not detectable until after 7 days of Dox treatment and increased thereafter. Immunohistochemical staining revealed PrP-positive dots and small deposits in at least some muscle cells (Fig. 4 F), arguing for the presence of PrP aggregates. Because Congo red staining is negative, the PrP aggregates in the Tg(HQK) mice are different from the Congo red-positive amyloid inclusions containing PrP, amyloid-β, and its precursor protein found in inclusion body myositis (3). Nevertheless, PrP aggregation might still play a role in the pathogenic process in the Tg(HQK) mice.
The muscle-specific PrP expression in the Tg(HQK) mice appears to be an intrinsic property of the HQK construct because all independent Tg(HQK) lines showed the same muscle specificity (data not shown). Therefore, the HQK construct has the potential to restrict the Dox-dependent expression of any gene to skeletal muscles when the ORF of this gene is used to replace the PrP ORF in HQK. Consequently, the HQK construct may be very valuable for generation of other regulated Tg mouse models of muscle diseases. It can also potentially be used for gene therapy through regulated expression of a specific protein lacking in muscle cells due to a gene defect.
Materials and Methods
The HQK Transgenic Mice.
The HQK transgene was created by replacing the mouse PrP ORF in the half genomic PrP (HGPRP) clone (12) with that of rtTA and placed upstream of tetO-hCMV*-human PrP ORF of the core plasmid (13) after a spacer sequence. The HQK transgene construct was microinjected into fertilized FVB/NJ eggs at Albert Einstein College of Medicine. Founder pups were screened for human PrP ORF by PCR as previously described (15). Human PrP ORF-positive founder mice were further screened by PCR for the rtTA ORF with primers rtTA-1 (CTTGGTGTAGAGCAGCCTAC) and rtTA2r (CCGCATATGATCAATTCAAGG). Selected founder mice were bred with FVB/Prnp0/0 mice to obtain Tg(HQK) mice that were HQK+/0/ Prnp0/0 . The FVB/Prnp0/0 mice were obtained from the Prusiner laboratory, where the Zurich I Prnp0 allele was from the Weissmann laboratory (14). The transgene expression in the brain and other tissues was examined by immunoblotting analysis by using 3F4 or 8H4.
Doxycycline Treatment.
Treatment of mice with Dox was done with rodent food from Bio-Serv (Frenchtown, NJ) containing indicated amounts of Dox (20 mg, 50 mg, 200 mg, 1 g, or 6 g per kg of food), and the food was changed three times per week. The dose of 6 g of Dox per kg of food was used except for the dose-response experiment.
Western Blot Analysis.
Tissues were homogenized and immunoblotted onto PVDF membranes after separation on 10–20% gradient Tris-Tricine SDS/PAGE gels (15). Total protein concentrations of the tissue homogenates were measured with the BCA Protein Assay Reagents (Pierce Chemical, Rockford, IL). Deglycosylation where indicated was performed with PNGase F (New England Biolabs, Ipswich, MA) following the manufacturer's instructions except for overnight incubation at 37°C. Immunoprecipitation of full-length PrP with mAb 8B4 was performed as described (27). The blots were probed with mAb 8H4 (for human PrP176–186 and mouse PrP175–185) or 3F4 (for human PrP109–112; Signet Laboratories, Dedham, MA) in conjunction with HRP-conjugated goat anti-mouse IgG Fc antibody (Chemicon International, Temecula, CA) and developed with BM chemiluminescence blotting substrate (Roche Diagnostics, Indianapolis, IN).
Total RNA was extracted from indicated tissues by using the RNeasy mini kit (QIAGEN, Valencia, CA). RT-PCR was performed with standard procedures by using primer pairs ACTTTGGCATTGTGGAAGGGCT and ACCCTGTTGCTGTAGCCGTATTC for GAPDH mRNA and GGATCAGCAGACCGATTCTG and GGTGACCGTGTGCTGCTTG for the transgene PrP mRNA. These primers are complementary to different exons of the respective genes.
Generation of the C1 Fragment.
The C1 fragment was generated by autolysis of brain tissue of the Tg40 mice expressing human PrP (15) as described (16), except that the brain tissue was autolysed at room temperature for 72 h.
Histological, Enzyme, and Immunohistochemical Analysis.
Skeletal muscles (quadriceps) were cryosectioned and subjected to H&E staining and enzyme immunohistochemical analysis by standard methods (28) for NADH-dehydrogenase, succinate dehydrogenase, and acid phosphatase. The sections were also stained by Congo red as described (29) and examined by using rhodamine optics to amplify the signal for congophilia. PrP immunostaining with 3F4 on paraffin-embedded, fixed-muscle sections was done as described (15).
We thank Dr. Stanley Prusiner (University of California, San Francisco, CA) for providing the FVB/Prnp0/0 mice derived from the Zurich I PrP-null mice and Dr. Wen-Hwa Lee (University of California, Irvine, CA) for providing the core plasmid. The study was funded by National Institutes of Health Grants AG14359 and NS052319.
- ††To whom correspondence should be addressed. Email: qxk2{at}case.edu
Author contributions: Q.K. designed research; S.H., J.L., M.Z., X.L., M.W., P.W., D.V., D.W., B.C., A.P.H., S.G.C., P.G., and Q.K. performed research; K.C. and M.C. contributed new reagents/analytic tools; S.H., A.P.H., S.B., M.C., P.G., and Q.K. analyzed data; and P.G. and Q.K. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
- Abbreviations:
- Dox,
- doxycycline;
- PrP,
- prion protein;
- rtTA,
- reverse tetracycline responsive transcription activator;
- Tg,
- transgenic.
- © 2007 by The National Academy of Sciences of the USA
- ↵
- Prusiner SB
- ↵
- ↵
- ↵
- ↵
- Caughey B ,
- Race RE ,
- Ernst D ,
- Buchmeier MJ ,
- Chesebro B
- ↵
- Chen SG ,
- Teplow DB ,
- Parchi P ,
- Teller JK ,
- Gambetti P ,
- Autilio-Gambetti L
- Taraboulos A ,
- Scott M ,
- Semenov A ,
- Avrahami D ,
- Laszlo L ,
- Prusiner SB
- Vincent B ,
- Paitel E ,
- Frobert Y ,
- Lehmann S ,
- Grassi J ,
- Checler F
- ↵
- ↵
- ↵
- Kong Q ,
- Huang S ,
- Zou W ,
- Vanegas D ,
- Wang M ,
- Wu D ,
- Yuan J ,
- Zheng M ,
- Bai H ,
- Deng H ,
- et al.
- ↵
- ↵
- ↵
- ↵
- ↵
- Moore RC ,
- Mastrangelo P ,
- Bouzamondo E ,
- Heinrich C ,
- Legname G ,
- Prusiner SB ,
- Hood L ,
- Westaway D ,
- DeArmond SJ ,
- Tremblay P
- ↵
- Nunziante M ,
- Gilch S ,
- Schatzl HM
- Harris DA ,
- Peters PJ ,
- Taraboulos A ,
- Lingappa V ,
- DeArmond SJ ,
- Prusiner SB
- Prusiner SB
- Shyng SL ,
- Huber MT ,
- Harris DA
- Vincent B ,
- Paitel E ,
- Saftig P ,
- Frobert Y ,
- Hartmann D ,
- De Strooper B ,
- Grassi J ,
- Lopez-Perez E ,
- Checler F
- ↵
- Dubowitz V
- Dubowitz V
- ↵


text:
CHICAGO (CBS) — The DeKalb County Crime Stoppers is offering a reward to gather information about two crimes that happened in the northwest suburb in the last two months.
The group is offering a reward of up to $1,000 for information about an attempted theft at Becky Beck's Jewelry, 303 E. Hillcrest Drive, and a burglary to a vehicle at a suburban apartment complex, according to a Crime Stoppers press release.
A man and a woman tried stealing jewelry at Becky Beck's Jewelry about 2:30 p.m. on Dec. 12 while the clerk was distracted, according to Crime Stoppers. They drove away in a brown or gray van.
The man was described as white, 6 feet tall and thin, with a teardrop tattoo on his face and wearing sunglasses, according to Crime Stoppers. There was no description of the woman.
The burglary happened overnight Nov. 7 at Suburban Apartments at 1400 Twombly Rd. in DeKalb, Crime Stoppers said. The owner of a 2005 gray Volkswagen Jetta reported that someone broke into his car and stole a large amount of cash.
Anyone with information about these crimes is asked to contact DeKalb County Crime Stoppers at (815) 895-3273. Callers can remain anonymous.
(Source: Sun-Times Media Wire © Chicago Sun-Times 2016. All Rights Reserved. This material may not be published, broadcast, rewritten, or redistributed.)


text:
Perfect getaway location. Great mountain range southeast yr-rd views. This Permitted for 4-BR modified mound septic system design.Ladies And Gents Bakersfield
Ready for your proposed home. FP, ski lockers, health club membership. Owner, Improved building lot in Killington neighborhood with ski home benefits. Call Looking to sell?
Our unique marketing plan features your very own website. Call, email or stop in. We are the red farm house located next to the Killington fuck Killington Barn. Marni peakpropertyrealestate. Next to 4-season motel www.Dat 360 App
Immediate business from motel guests. Turn-key operation for restaurant, bakery catering. Small or large square footage.
Killington is owned by morons [Archive] - Teton Gravity Research Forums
Close to ski shop, restaurant and lodging. Great location for any business. Nice neighborhood off Rt. Bedrooms have private baths, no bunks, two per room, singles or killington fuck Killington, on the Killinggton Rd.
Send inquiries to tlr gmail. Beautiful 6BD, outdoor hot tub, close to everything!Mean Jokes To Say To A Boy
Full or half shares. We have two teens. Dec to April. Call Sue at Short walk to lesbian dating site killington fuck Killington. Call Louise Harrison, Off street parking. Great back yard! Judy Fuckk Nov 1 thru April Fully furnished and equipped top floor. All utilities included! Andrea Weymouth, Owner.
Killington fuck Killington Seeking Adult Dating
Includes. No pets. Jamie This newspaper will not knowingly accept any advertisement which is in violation of the law.
Our readers are hereby informed that all dwellings advertised in this newspaper are available on an equal opportunity basis. Excellent, reasonable, must-see.
Killington fuck Killington Searching Sex Contacts
Rutland Used one winter season. Call Dotty Must pick up. Free estimates.Adult Phone Chat Westerly
No obligation. Royal Barnard Busy Killington bike shop looking for part-time seasonal help. Start immediately. Experience preferred, but training available for the right persons. Competitive salary DOE.
Send qualifications, availability and contact info to tracy snowsportsmarketing. Please callemail killinhton stop in killington fuck Killington an application. The Birch Ridge Inn at Killington seeks innkeepers assistant for house keeping and breakfast service. All positions. Training, uniforms, perks provided.
I Wanting Sexual Dating Killington fuck Killington
Uniforms, free meal and other perks provided. Contact Lauren Skaskiw at Competitive wage. Please call Experience would be great, but if you enjoy working with food, we will train. Year-round position, M-F. killington fuck Killington
Access to ski pass. Apply in person at Killington Deli, Route 4.
Brooke Geery - Tag Archive - sex in the killington gondola
Must have valid drivers license and be able to work overtime during winter months for snow removal. Contact Jim at Killijgton interview. Killington fuck Killington to submit a classified? Email classifieds mountaintimes. Rates are 50 cents per word, per week; free ads are free.
Weekly Classifieds: Dresser, bureau, 2 night tables. Frank, Rudi, Bob, Never killington fuck Killington. Matty, Call Jeff at First Impressions, killingfon Drivers license required.
Call Inn at Long Trail for interview.


text:
A nice Pad
The place to upload Harmor presets and discuss programming.
Return to "Harmor - Presets & Programming"
2 posts • Page 1 of 1
[You can only see part of this thread as you are not logged in to the forums]
A nice Pad
You do not have the required permissions to view the files attached to this post.
Re: A nice Pad
sounds amazing! I just think it is phasing too ...
2 posts • Page 1 of 1


text:
This year I had a chance to get to Bane of War in Austin Texas. It was my first trip to Texas and was keen to get there and have look at the local scene and get a bit of wargaming in.
Warlord Games Kradschutzen Platoon
I started work some time ago on a Bolt Action platoon of Warlord Games Kradschutzen. Motorcycle troops were all around the early and mid periods of the North Africa theater. These troops were the fast moving Infantry and recon for the desert DAK forces.
DBA 3.0 :- Review
DBA was my first entry into Ancients, I had not played any ancients before and was keen to give them ago, with out wanting to jump in the deep end and get going WRG or DBM at the time a whole group of us looked at BDA.
Warlord Games Panzer IIc
What I have finished.
I have been working on a Warlord Games Panzer IIc for Bolt Action.
The Warlord Games Panzer IIc I have is a resin model with left and right Track, Hull, and Turret. with metal Barrels for the MG and 2cm main-gun.
Overall the quality is fantastic. I wish I had of started this blog back when I was first cleaning and assembling it, for photos. Like with all resin models I get I clean with warm soapy water to remove any mold release and other residue from casting, then dry fit everything before I start with paint.
Welcome to What could Possibly go wrong!
Welcome to What could possibly go wrong! ,
Let me say that firstly I am a gamer, I enjoy gaming in all periods and scales. I like different rule sets because I just like them. But I also tend to play what people are playing, I find it pointless to purchase a rule set miniatures and have nobody to play. I have in recent years moved on from some game systems, finding them a little childish or just plain boring and not capturing my attention like they used to.


text:
A well proportioned, purpose built one bedroom, first floor maisonette in this sought after Bucks village - a great opportunity for the First Time Buyer or Rental Investor.
A beautifully appointed newly built detached residence enjoying spectacular views. Exceptional specification and located in an idyllic setting adjoining open fields .
A unique country residence situated in a secluded and picturesque location with outbuildings and stables, in all 5.8 acres.
PART EXCHANGE AVAILABLE Hunters are pleased to present this FOUR bedroom DETACHED family home with a ONE BEDROOM SELF CONTAINED ANNEX in this exclusive modern new home development in the sought after village of Aston Clinton. The ground floor accommodation boasts a large open plan part oak framed li
A two bedroom bungalow over looking the green and to be sold with no upper chain.
A substantial detached home offering spacious accommodation occupying a large corner plot with private garden within this highly regarded village setting.


text:
Quick Links
Daymohn Laws has organized a sock drive to benefit the homeless. He's collected donations through social media, and he plans on continuing past what he has already gathered. His goal is to personally hand out socks to people who need them throughout Sacramento.
Click Here to check out the full story.


text:
How long do i cook chicken thighs on stove top
Absolute how long do i cook chicken thighs on stove top
Melynda Sorrels spent 10 years in the military working in different capacities of the medical field, including dental assisting, health services administration, decontamination and urgent medical care. Can you drink whole milk on the keto diet.
How On the road to Prepare Apprehensive concerning A Pitch Flatten Skillet - Like a dream Fit for human consumption Reluctant Thighs Canister i handling my travel document debt souvenir membership card on the net.
- I have not giving up on love lyrics andy grammer - How to make good slime easy
- Perfect Cast-Iron Skillet Chicken Thighs Recipe | Bon Appetit
- 3 Ways To Cook Juicy Chicken Thighs | Kitchen Basics by FlavCity
- Juicy Stove Top Chicken Thighs | Easy Chicken Thighs Dinner Recipe
- However, this stove top chicken thighs recipe is about to change all of .. chicken – did you do anything...
- This method for coking chicken thighs involves a cast iron pan, the...
- However, the bone adds thickness to the meat, so it requires more cooking time to reach the...
Pictures pics of cute couples with love quotes. Lg 22 cu ft counter-depth 4-door french door. Meal prep for weight loss female recipes.
Hollywood movie hindi picture downloading website name. Mp3 cutter and joiner app for pc download. How do you make smores bars. Happy new year comedy photo 2020 hd wallpaper shayari. New photo 2020 video song dj hd download mp3 from youtube. Can you get text messages if your phone is on airplane mode.
Best ways to learn spanish at home. How to put photos onto icloud from iphone. How to remove amazon pop up on android phone. How to use your instagram account back after being hacked. Www photo in com hindi song new 2020 video download dj king. Red rose pic single. Now you see me 2 streaming français hd hindi download 300mb. Can you put a metal tray in a convection oven.
Free download fb auto liker app for android. Appareil photo full format numérique petite. How long to quit smoking on chantix. Pictures of psoriasis on scalp hair loss. How to change xfinity wifi router password. Healthy dinner recipes the whole family will love. How to cook lamb leg roast in slow cooker. World cup news and photo 2020 france wins football. Happy birthday video song with name free download mp4. What not to eat to lower blood pressure.
How to make cool fast paper airplane that flies far step by. All movie hindi songs new dj 2020 mp3. New photo 2020 video song hindi download free.
Pic of real 100 dollar bill. Jurassic world picture download movie in hindi hd khatrimaza.com. Happy new year images tamil.
Image to pdf windows 8.1 64 bit download full version. How to change mail account on iphone 8. How to cook bbq beef in crock pot with cabbage. Picture of a heart shaped box lyrics nirvana meaning. Ways to lower high blood pressure during pregnancy. Is it smart to buyout a leased car. How to make bread and butter pickles crunchy. Best gaming earbuds with mic 2020. How to get cool emojis for iphone.
How to make a paper airplane fly straight. Best place to buy moving boxes reddit. Can you use microwave safe bowls in the oven. Wireless headphones under 10k. How to factory reset iphone 5 without passcode and computer. How to make a photo collage on iphone xs max. How do u know if someone screenshots your snapchat story. How long do you bake stuffed chicken breasts at 350. How many cups of water to 2 of basmati rice.
Walmart locations in maine usa. Picture hd song bhojpuri video download 2020 dj0 new. How you know when someone love. How to add text to a picture on my ipad. Adobe photoshop elements 8 mac serial number. Us passport renewal 2019. See for yourself in spanish. How to cast my samsung tablet to my tv.
Perfectly golden, tender, and juicy skinless, boneless chicken thighs prepared on the stove top. Joining mp3 free downloads hindi old.


text:
Nicole Tovar, a Culinary Arts major and a Staff Sergeant with the U.S. Army Reserves, has a mission: to unite the student veteran population at UAA.
As president of the Student Veterans of UAA, a support group for veterans on campus, Tovar hopes to assist veterans who are struggling to find resources and figure out their military benefits, among other difficulties. Such a resource has been lacking for veterans at UAA—leaving a large percent of the campus unrepresented.
Tammy Cartwright, a combat veteran from Operation Iraqi Freedom, initially brought Student Veterans of UAA to life in the fall of 2010. The group struggled to attract members, however, and when personal matters forced Cartwright to step out of the group completely, Tovar took up the reins.
"They were struggling to get things up and running," said Tovar. "Which, when you're attempting to bring together a group of inherently solitary veterans, that's understandable. So I decided, 'Well, I'll give it a shot.'"
Joining her as vice president of the club is Amanda Doyle, a Nursing major and a Sergeant in the Army Reserves. She was inspired to take on the role, she said, when she realized just how few veterans knew and were involved with Student Veterans.
"We're really missing that sense of community among our veterans here," Doyle said. "With all the veterans I know on campus, it's sad that we're not showing unity at all."
Tovar and Doyle's plan is to build that sense of community back up.
"We're here with the resources veterans are looking for as they try to make it through college," said Doyle. "Hopefully we can help them come together."
"There are a lot of things that need to be done," Tovar stated. "We've been in contact with the school a lot, trying to rebuild bridges and establish contacts with departments like the financial office."
Their biggest accomplishment so far has been to connect with the Student Veterans of America, a national organization that runs a highly organized network for student veterans. The organization has provided Tovar with many resources for Student Veterans of UAA, including access to scholarships, GI Bill update information, and an internship support program which gives veterans a monthly stipend to complete internships while attending school.
"Student Veterans of America has been training us, and now we can turn around and give that information to those who need it," said Tovar. "We've got access to all these great programs, so we can really provide legitimate support to our veterans on campus."
The club has big plans for this coming November. What originally was planned as a Military Appreciation Day has now expanded to a weeklong event, set to feature a resource fair, basketball game, and a Veterans panel at the library. The main event will be a full military ceremony, involving a six-hour name reading of all the fallen soldiers and a national moment of silence for which UAA will be representing the state of Alaska.
"The ultimate goal is to get the word out, spread awareness and get our veterans united," said Tovar. "This Military Appreciation Week is just the beginning of what we hope to accomplish."


text:
4SCT_VespaFounders [premium]
Content Count217
Last visited
Community Reputation52 Excellent
About 4SCT_Vespa
Contact Methods
Website URL
Profile Information
Sacile (Italy)
Recent Profile Visitors
IL2 doesn't start anymore
4SCT_Vespa replied to 4SCT_Vespa's topic in Technical Issues and Bug ReportsNo way to fix this issue...and seems that 1C doesn't care about that. I just have to remove this poor game and switch to DCS
Sent a new PM....Just in case you won't get it...can I sen to you an email? Thanks
Thanks for update Buzzsaw...I can't wait to get TF. By the way...you got a PM
Thanks a lot for info Sokol, now I know that I don't need to buy IL2 CoD Blitz or whatever...but I just have to wait for Tobruk 5.0
Can't wait to get this MOD. I have a question since I'm totally new to IL2 Cliffs of Dover: is this a completely standalone game? I mean...what are the step to install this MOD ( when it will be available ) or any other IL2 CoD MOD? Thanks
IL2 doesn't start anymore
4SCT_Vespa replied to 4SCT_Vespa's topic in Technical Issues and Bug ReportsThanks for feedback Thad; I already tried to start the game clicking on the IL2.exe file, as Admin. Same result I'm really get crazy...the game was working fine till few days ago....
IL2 doesn't start anymore
4SCT_Vespa replied to 4SCT_Vespa's topic in Technical Issues and Bug ReportsNo good luck. Here you are what I did: - Uninstalled the game - Manually deleted all the folder not remove by uninstaller - Performed a registry clean using CCleaner - Turned off the PC - Turned on the PC - Re installed the game Nothing: the launcher can run and install all the files...but when I click PLAY the game doesn't start.
IL2 doesn't start anymore
4SCT_Vespa replied to 4SCT_Vespa's topic in Technical Issues and Bug ReportsI have uninstalled the game...then manually deleted all the data but not restarted the game before re-installing. I'm going to to do....SOB! 11 Gb. Thanks for hint...I'll report about success or not
IL2 doesn't start anymore
4SCT_Vespa posted a topic in Technical Issues and Bug ReportsHi guys, I got in trouble with IL2 after latest WIndows 10 update. The game doesn't start anymore; I mean, I can run the launcher, I can update the game but when I click PLAY I see the IL2 icon in the Windows bottom bar...but the game doesn't start. I've set up the game to run as Admin...of course. I tried to uninstall and reinstall the game....nothing. In the Windows program manager I see the Launcher and IL2 but I can just close the program from there... Any help?
Thanks a lot Acasto, I really appreciate your efforts and support.
What a great template! Thanks a lot for your great work. I'm totally newbye in 2D art...but I'll try to do a Regia Aeronautica Ju87 D3 historical livery. Just a question about normal map downloadable from the first post...I'm not sure what to do with thaty...do I need to place it inside the custom livery aircraft folder? Thanks for any hint and feedback
Custom skins and multiplayer
4SCT_Vespa replied to 4SCT_Vespa's topic in Skins and TemplatesI got it. Thanks a lot FlyingShark
Custom skins and multiplayer
4SCT_Vespa posted a topic in Skins and TemplatesFirst of all sorry if this topic has been already discussed; I tried to do a quick search but got no previous post. The question is: are custom skins viewvable by other players in multiplayer? Is this a feature implemented in IL2? Thanks for any feedback
Macchi C.202 Folgore 4k (409gx4096) Work in Progress
4SCT_Vespa replied to ICDP's topic in 4K Skins and TemplatesGreat work...as usual
M.C. 202 Series 8 Skins
4SCT_Vespa replied to Jason_Williams's topic in Skins and TemplatesThanks for your efforts Jade


text:
Main Content
Session Information
33 SES 08 B, Science, Engineers and Education - A Gender Perspective
Paper Session
Adolescents' career plans are indicative of their future educational and also labour market decisions. These associations also hold for employment in Science, Technology, Engineering and Mathematics (STEM) which is an area of particular interest not only for its high importance for countries' economic growth, but also for the massive and non-decreasing gender-segregation in the field. To better understand why men and women end up studying certain subjects and doing certain jobs, we need to look at how they develop their career plans at early ages.
On the individual level, science and maths performance are strong predictors of STEM career choices. However, it is not only the actual performance that matters: students' attitudes toward science and maths are also important. In particular, subject self-efficacy (the extent to which students believe that they can handle given subject-related tasks effectively) and enjoyment of science (intrinsic interest or interest value) have successfully been linked to STEM career plans and also to STEM higher education choices.
Concerning enjoyment, single-country studies and also studies that combine several countries have demonstrated that students, who enjoy doing science and have more interest in the subject are also more likely to opt for STEM also when their abilities and self-efficacy are held constant. Moreover, there is some evidence that gender-differences in enjoyment (more broadly: subjective task values) related to math and physical sciences explain some of the gender gap in the corresponding occupational choices as girls tend to attach less intrinsic value to STEM-related subjects.
Our preliminary analyses of PISA 2015 data however demonstrate that the underlying processes that link science enjoyment to related career choices show complex patterns not only by gender but also by country. In particular, we find that in some European countries, the association between science enjoyment and STEM career choices is either consistently positive across genders (e.g. Austria, Belgium, Denmark, Germany, Italy, Sweden, the UK…), or it is more pronounced for males (France, Ireland and the Netherlands). In a second group of countries however, enjoyment of science is either unrelated to STEM choices (e.g. Bulgaria, the Czech Republic, Latvia, Romania…), or – in a smaller number of cases – shows some positive effect for females only (Croatia, Estonia, Slovenia).
The lack of relevance of science enjoyment either for both genders or for males in the second group of countries is an interesting phenomenon. At this stage we can only speculate about its roots. As it is mainly countries with lower income that belong to this group, we propose that in less affluent societies, students might be less concerned with intrinsic than with extrinsic rewards of their future jobs as their major concern is to achieve labour market security and also a high income. As STEM occupations offer high returns across Europe, it is possible that in poorer countries, students – with a given level of achievement and self-efficacy – are more inclined to choose such careers irrespective of their interest for the topic. An alternative hypothesis however suggests that it is part of the socio-economic heritage of the post-socialist past that disconnects enjoyment and career choices in a high number of European countries.
The paper will explore the complex associations between enjoyment of science and STEM career choices of the students, and reveal the role of gender and some country-characteristics in moderating these associations. We aim to better understand whether making science learning more enjoyable for students can boost students' interest in related careers and whether such attempts can also help to reduce the gender segregation in STEM. We expect that the answers to these questions to show remarkable cross-country differences.
PISA data includes comparable information on occupational expectations of adolescents as well as test-scores on students' science performance and variables related to their attitudes for science. From these, students' science self-efficacy (their self-beliefs whether they could perform a series of science-related tasks either easily or not) and the enjoyment of science scale will be used. The latter was constructed from five items describing students' enjoyment in activities related to science. Gender will be depicted by a dummy variable. On the country level, two alternative measures will be used to identify the underlying cross-country differences: Human Development Index (HDI) will account for the different levels of affluence between the countries and a dummy for a socialist past will depict potential cultural and historical factors. The measure of student career expectations was constructed from the following question: What kind of job do you expect to have when you are about 30 years old? Our definition of STEM occupation is based on ISCO-08 codes and includes the following job-categories: Science and engineering professionals; Information and communications technology professionals; Science and engineering associate professionals; Information and communication technicians. A dummy variable was constructed with STEM occupations coded as 1, and 0 otherwise. In the analyses, PISA 2015 data for the European countries will be used. To deal with missing values, multiple imputation by chained equations (ICE) was applied. The analysis will employ the General Multilevel Structural Equations Modelling Framework for assessing multilevel mediation and moderation. With this framework we are able to separate between effects (country level associations) from within effects (individual level associations). In the analyses we will focus on relations between STEM career choices of individuals, science score, self-efficacy and enjoyment and gender. In moderation analysis we will explain the cross country variation of relations between predictors and dependent variables using country level variables, which will be the Human Development and distinction between post-socialist and non-post-socialist countries. Mediation analysis will investigate how the relation between gender and STEM choices is mediated by enjoyment.
Expected Outcomes
We expect that between-country differences in HDI and the historical past of the European countries explain between country differences in the relationships between science-enjoyment and STEM career choices also when students' science performance, their self-efficacy and other control variables are held constant. Further, these relationships will also vary by gender. In European countries with a post-socialist heritage (or with lower levels of HDI) students' enjoyment is a weaker predictor of their interest in STEM occupations than it is other countries. If at all, a positive association between science enjoyment and STEM career choices exists among females, but not among males. On the other hand, in countries without a socialist past (or in the more affluent countries of Europe), science enjoyment remains a strong predictor of students; related career choices and this association is particularly strong among males. We expect the post-socialist factor to be a stronger predictor than HDI and we will present possible explanations related to the different socio-historical paths of the two groups of European countries. The presentation will also discuss policy implications.
Bandura, A. (1977). Self-efficacy: Toward a Unifying Theory of Behavioral Change. Psychological Review, 84(2), 191–215. Bandura, A. (1999). Social cognitive theory: An agentic perspective. Asian Journal of Social Psychology, (2), 21–41. Blasko, Zs., Pokropek, A. & Sikora, J. (2018): Science career plans of adolescents: patterns, trends and gender divides. JRC Science for Policy Report. European Commission, JRC 2018 Eccles, J. (2009). Who Am I and What Am I Going to Do With My Life? Personal and Collective Identities as Motivators of Action. Educational Psychologist, 44(2), 78–89. https://doi.org/10.1080/00461520902832368 Eccles, J. (2011). Gendered Educational and Occupational Choices: Applying the Eccles et al. Model of Achievement-Related Choices. International Journal of Behavioral Development, 35(3), 195–201. Eccles, J. S. (1994). Understanding women's educational and occupational choices. Psychology of Women Quarterly, 18(4), 585–609. Goos, M., Hathaway, I., Konings, J., & Vandeweyer, M. (2013). High-technology employment in the European Union. Retrieved from https://lirias.kuleuven.be/handle/123456789/445755 Han, S. W. (2017). What motivates high-school students to pursue STEM careers? The influence of public attitudes towards science and technology in comparative perspective. Journal of Education and Work, 30(6), 632–652. https://doi.org/10.1080/13639080.2017.1329584 Koller, O., Baumert, J., & Schnabel, K. (2001). Does Interest Matter? The Relationship between Academic Interest and Achievement in Mathematics. Journal for Research in Mathematics Education, 32(5), 448. https://doi.org/10.2307/749801 Mcmaster, N. C. (2017). What role do enjoyment and students' perception of ability play in social disparities in subject choices at university? UCL Working Paper. Riegle-Crumb, C., Moore, C., & Ramos-Wada, A. (2011). Who wants to have a career in science or math? exploring adolescents' future aspirations by gender and race/ethnicity. Science Education, 95(3), 458–476. https://doi.org/10.1002/sce.20431 Royston, P., & others. (2004). Multiple imputation of missing values. Stata Journal, 4(3), 227–41. Sikora, J. (2014). Gendered pathways into the post-secondary study of science (Occasional Paper). NCVER, Australian Government. Tai, R. H., Liu, C. Q., Maltese, A. V., & Fan, X. (2006). Planning early for careers in science. Science, 312(5777), 1143–1144.
Programme by Network 2019
00. Central Events (Keynotes, EERA-Panel, EERJ Round Table, Invited Sessions)
Network 1. Continuing Professional Development: Learning for Individuals, Leaders, and Organisations
Network 2. Vocational Education and Training (VETNET)
Network 3. Curriculum Innovation
Network 4. Inclusive Education
Network 5. Children and Youth at Risk and Urban Education
Network 6. Open Learning: Media, Environments and Cultures
Network 7. Social Justice and Intercultural Education
Network 8. Research on Health Education
Network 9. Assessment, Evaluation, Testing and Measurement
Network 10. Teacher Education Research
Network 11. Educational Effectiveness and Quality Assurance
Network 12. LISnet - Library and Information Science Network
Network 13. Philosophy of Education
Network 14. Communities, Families and Schooling in Educational Research
Network 15. Research Partnerships in Education
Network 16. ICT in Education and Training
Network 17. Histories of Education
Network 18. Research in Sport Pedagogy
Network 19. Ethnography
Network 20. Research in Innovative Intercultural Learning Environments
Network 22. Research in Higher Education
Network 23. Policy Studies and Politics of Education
Network 24. Mathematics Education Research
Network 25. Research on Children's Rights in Education
Network 26. Educational Leadership
Network 27. Didactics – Learning and Teaching
Network 28. Sociologies of Education
Network 29. Reserach on Arts Education
Network 30. Research on Environmental und Sustainability Education
Network 31. Research on Language and Education (LEd)
Network 32. Organizational Education
Network 33. Gender and Education
Additional Events
The programme is updated regularly (each day in the morning)
Marginal Content
Search the ECER Programme
- Search for keywords and phrases in "Text Search"
- Restrict in which part of the abstracts to search in "Where to search"
- Search for authors and in the respective field.
- For planning your conference attendance you may want to use the conference app, which will be issued some weeks before the conference
- If you are a session chair, best look up your chairing duties in the conference system (Conftool) or the app.


text:
Local Storage seems to be disabled in your browser.
For the best experience on our site, be sure to turn on Local Storage in your browser.
Xenoblade Chronicles 2
- Language
- Suggestive Themes
- Use of Alcohol
- Violence
Lead Rex, Pyra, and the World to Refuge!
As the giant beasts march toward death, the last hope is a scavenger named Rex — and Pyra, a living weapon known as a Blade. Can you find the fabled paradise she calls home? Command a group of Blades and lead them to countless strategic victories before the world ends.
Each Titan hosts its own distinct cultures, wildlife, and diverse regions to explore. Search the vast open areas and labyrinthine corridors for treasure, secret paths, and creatures to battle and index.
During these escapades you'll get to know a large cast of eclectic characters, including the weaponized life forms known as Blades. Gather these allies, bond with them to increase their power, and utilize their special ARTS to devastate enemies. But to save the world of Alrest, you must first demystify its cloudy past.
|Country of Origin
|Nintendo Switch, Nintendo Switch Lite
|Release Date
|Nov 29, 2017
|No. of Players
|Monolith Soft


text:
Drcorydrake needed a new logo design and created a contest on 99designs.
A winner was selected from 91 designs submitted by 31 freelance designers.
Unicorn Surf Company
Because we all feel like mythical magical creatures when wakesurfing
Surfing- family friendly, middle aged guys acting like kids
Want a retro look
I want to create a character based off of a buffalo
I am from South Dakota and feel a Buffalo would be a good mascot for our company. Our business is smiles. I kind of imagine the buffalo being head on
Find out how a design contest works or learn more about our logo design services.


text:
Boat sightseeing, With a guide Trips to Emårsøy. In the summertime you may join in on a boat trip to the beatiful island Emårsøy. Travel back in time and experience how it was to live on a small farm on an re... Read more
Activities, Local sightseeing, Historical walks [...] Welcome to an inspiring and invigorating holiday stay in Helgeland's archipelago! Tømmervika offers guided coastal kayak excursions (1–6 days). We explore Helgeland's magnificen... Read more


text:
Russia the Enemy—Not Friend A friend is an ally, someone who is on your side. But it goes deeper than that. A true friend is one who seeks to promote your happiness and prosperity. An enemy is someone hostile to you; one who works to harm you and your best interests. By any set of… More
Real Americans
The Faux Americans Donald Trump has given hate groups license to unabashedly reveal their hatred of everyone different from them—the Others. White supremacists and their nationalist, Neo-Nazi and alt-right allies have taken advantage of their newfound legitimacy. They publicly and proudly proclaim they are the Real Americans. The rest of us, with our different skin… More
I love our country. Do you?
The Criminal in the White House I love our country but something terrible is happening here. And it's hard for me to believe we, the majority of Americans, like it. No matter our political leanings, we did not vote to put a criminal in the White House, nor do we now approve of the one… More
Trump, Lackeys and the White Rose
Republican Lackeys and the White Rose Lackeys are persons who show servile obedience or deference toward someone else without questioning them. Republican Lackeys supporting our crude, racist, uninformed, misogynistic President are creating a precarious situation for the United States. "Nothing is so unworthy of a civilized nation as allowing itself to be governed, without opposition,… More
Trump's Foreign Policy is Narcissism Driven Chaos
Presidential Doctrines Most Presidents come to office with their own ideas about foreign policy. Some even put them in writing in the form of a doctrine. President James Monroe issued the first Presidential Doctrine in 1823. The Monroe Doctrine was a statement of American foreign policy. It warned European powers not to further colonize in… More
D-Day June 6, 1944 – 75 Years Later
World War ll – The Western Front It is June 1944. The United States is in its third year as a combatant in the Second World War. Nazi Germany controls much of Continental Europe. For three years the Soviet Union has been engaged in a violent battle with Germany in the east. It is pressing… More
Donald Trump and the Rise of Populism
Trump's Brand of Populism Many people have wondered how Donald Trump attracted a hard core of followers despite all that was known about him. There were those who came to his side for strictly ideological reasons. Some were evangelicals who came en masse based on Trump's promise to appoint conservative judges to overturn Roe v.… More
Russian Meddling – Don't Talk About It
Don't Talk About Russian Meddling Don't talk about Russian meddling in our elections. That was the essence of the warning Acting White House Chief of Staff Mick Mulvaney gave then-Homeland Security Secretary Kirstjen Nielsen earlier this year before her meeting with the President. Several news sources reported Mulvaney told her it "wasn't a great subject… More
2020 Democrats — Flashing Warning Signs
Misreading the results It's easy to understand how a big electoral victory can instill a high confidence level. But such a large victory can also lead to a misreading of its impact on future results. That is particularly true when the warning signs start flashing and many Democrats seemingly ignore them. But history has repeatedly… More
Snatching Defeat from the Arms of Victory
The 2016 Election In 2016 Hillary Clinton was the odds-on favorite to win the presidential election. Yet something happened on the way to the ballot box, and she snatched defeat out of the arms of victory. Granted, Russian interference in the election promoting Donald Trump certainly cost her votes in several key states. As did… More


text:
Ack wrote:I don't know, chief, the haunting feeling of lust I feel whenever I look at your avatar makes me think it's real.
strangenova wrote:Happy Easter everyone. Just had an egg hunt at my grandmother's for my son, about to go to a crawfish boil for the evening.
Users browsing this forum: No registered users and 16 guests


text:
You may also like. Despite the X83's lack of fax hardware, it smoothly acts as a front end for PC-based faxing. Lexmark X83 Sign in to comment Be respectful, keep it civil and stay on topic. On plain paper, text looked good, but quality dipped slightly. Write a customer review. The bit flatbed scanner easily handles books, reports, and more. At least Lexmark isn't skimping on support.
|Date Added:
|9 February 2005
|File Size:
|6.96 Mb
|Operating Systems:
|Windows NT/2000/XP/2003/2003/7/8/10 MacOS 10/X
|Free* [*Free Regsitration Required]
Originally, this was a perfect printer for our needs.
Sometimes the HP misses outlines altogether, the light grays fade into a non print, and the Lexmark captures all of that. Feedback If you are a seller for this product, would you like to suggest updates through seller support?
This Lexmark thermal printer with a print noise level of 45dBA functions almost noiselessly. That was the last straw. Lexmark X83 – multifunction printer color Series Sign in to comment Be respectful, keep it civil and stay on topic.
Lexmark X83 Specs – CNET
Please try your search again later. The Lexmark offers x dpi on printing. Finally, a multifunction inkjet printer with low-cost ink refills Finally, a multifunction inkjet printer with low-cost ink refills by Justin Yu.
Amazon Inspire Digital Educational Resources. The Scan button puts the X83 on standby until you fire up the Lexmark control software, while Scan To lets you control x833 operation from the hardware. Don't show this again. Amazon Second Chance Pass it on, trade it in, give it a second life.
Lexmark X83 Driver X
Learn more about Amazon Prime. Technical Specification User Manual [pdf ]. Amazon Rapids Fun stories for kids on the go. Using the multifunction is easy and intuitive. The Lexmark Web site also offers driver downloads, FAQs, user guides, an interactive knowledge base, and e-mail support. We'd call the quality of the copies good overall rather than excellent because of a slight dip in the sharpness of text reproduction on plain paper–but it's better than that of many other inkjet multifunctions we've tested.
Input tray – sheets – 8. My wife, kids, and I each have XP "accounts" so that we can each have lex,ark own files, preferences etc.
Lexmark X83 – multifunction printer ( color ) Series
See and discover other items: Mark Zuckerberg on Facebook's I wanted this specific model printer to use up excess ink i had after my first printer stopped working. It involved rebooting the computer then immediately printing, turning the printer on and off, etc. I figured the best way was to buy the same type so all she needed to do is unplug the old one and plug in this one.
Enjoy photo-quality black and color printing and bit color flatbed scanning. I would suggest trying a different printer. The Lexmark X83 more than held its own against the competition, delivering the fastest text output of scannrr three multifunctions we compared.
Lexmark X83 review: Lexmark X83 – CNET
Get to Know Us. However, lxemark doesn't have its own fax capabilities, working instead with your PC's modem to complete this task. Had difficulty with other models operating with the computer. East Dane Designer Men's Fashion. But most scanning operations can be executed from the hardware itself. I can't compare this with fore it's the only one I've owned or used.


text:
About Sweatsedo
Choose all the fresh Sweatsedo Coupon & Coupon Code is our daily activity. Saving the Sweatsedo Coupon and Coupon Code for a huge savings! Visit sweatsedo.com and saving now, You will save a lot compared to before with great Sweatsedo Coupon, Coupon Code and deals for January 2020 by hotdeals.com.


text:
Job Description
Brook Street has an immediate requirement for a Purchase Ledger Clerk to join an established, global business in Stafford Park, Telford.
The experienced Purchase Ledger Clerk would be required ongoing, on a temporary contract working 37.5 hours per week.
The team are really helpful and will provide full training to enable you to work together in a friendly environment.
Some of the duties will include:
" Purchase Ledger
" Communicate through Outlook
" Using SAP to access all vendor accounts
" Matching delivery notes to invoices
" Processing invoices
" Dealing with supplier and invoice queries
" Supplier statement reconciliations
" Daily post inc. overdue reminders, cheques
" Ad hoc duties within the team
Key Requirements:
" Previous Purchase Ledger experience
" A self-motivated individual and willing team player
" Excellent attention to detail
" Computer literate with advanced knowledge Excel
" Excellent communication skills
" Ability to work under pressure and to deadlines
Free parking and canteen facilities available.
Contact Brook Street for further details 01952 299591 or register online NOW!


text:
- Kitchen
- Home
- Health and Wellness
- Sale
- Learn More
Your Cart is Empty
Easy-to-use, just stake the StarTastic laser moving lights projector into the ground in front of your home and enjoy a spectacular light show! Great to decorate or home or landscape. A dusk-to-dawn sensor means it turns on automatically at night, and off by day.
how big an area does on light fixture cover
It will cover an area up to 4000 sq. feet
does this item go on and off on its own once you turn it on
This item must be turned on an off manually.


text:
Beet Bikores (11-18)
Beet Bikores. Round, dark red beet; good solid color throughout the entire beet. Thin skin. Very sweet. Resists bolting. 7 gram packet. Approximately 40-60 seeds per gram. To see our growing...
An heirloom that is standard for the home gardener. Dark red flesh, smooth skin, sweet flavor. 3" beets. 6 gram pack. Approximately 40-60 seeds per gram.
To see our growing guide for beets, click here.


text:
25 year guarantee. Read about the terms in the guarantee brochure.Made from cast iron, which spreads heat evenly through the pan and retains the heat for a long time...
Package Quantity
These additional services are only available in certain districts of Istanbul and for orders of a certain weight. Click for details.
Length: 39 cm
Width: 26 cm
Volume: 5 l
25 year guarantee. Read about the terms in the guarantee brochure.
Made from cast iron, which spreads heat evenly through the pan and retains the heat for a long time. This makes it ideal, for example, for slower cooking such as roasts and casseroles.
Steam collects on the inside of the lid and drips down from the knobs. In this way the food bastes itself, which gives a succulent and tasty result.
Works well on all types of hobs, including induction hob.
The pan doesn't have to be oiled and is easy to maintain thanks to the matt enamelled interior.
The pan has extra thick walls and base, which distribute the heat evenly and give good cooking results.
Shiny enamelled exterior makes the pan easy to clean.
Can also be used in the oven since it is made entirely of metal.
Using the lid brings the contents of the pot to a boil faster, so that you can save time, energy and money while lowering your environmental impact.
Complete with pot stands.
Handwash only.
Suitable for use on gas hob.
Suitable for use on induction hob.
Suitable for use on glass ceramic hob.
Suitable for use on cast iron hob.
Cast iron, Enamel
You can choose a store and check the stock status* for the product at the nearest IKEA.
The orders given at {0} are supplied from stock of {1} store
Aşağıdaki ürün/ürünlerin şu an İnternet Mağazasında stoğu bulunmamaktadır. Stoğa gelince haber ver butonunu kullanabilir ya da size en yakın IKEA mağazasından satın alabilirsiniz.
Sadece IKEA Mağazalarında
Aşağıdaki ürün/ürünler İnternet Mağazasında satılmamaktadır. Bu ürünleri size en yakın IKEA mağazasından satın alabilirsiniz.


text:
"'Oh no!' I apologized for having the wrong number. He said, 'No worries. How is your day going?' The more we talked, the more I liked him. But deep down it all seemed so weird. What if he was a creep? I don't even know him! It was just an online friendship. Or so I thought. My mom met him first. 'You're going to marry this guy!' What!?!??"
'Is it OK to call you?' What if he's a creep? I don't even know him! Mom said, 'Why don't I meet him first?': Woman meets future husband through accidental text while living in another state
'You can't put me in ICU Room 6. My mom just died there last week.': Young woman miraculously survives pulmonary embolisms just one week after mom dies of 'broken heart'
"I lost my best friend. 'What am I supposed to do now?' All I'd ever known was taking care of my mother. Suddenly, I felt a tightness in my chest. 'Well here is the panic attack I've been waiting for since she died.' I arrive at the ER. The doctor came in. I remembered her. She was the doctor who admitted my mom. My heart rate shot up in panic. I looked her right in the eyes. 'You can't put me in Room 6. My mom just died there last week.'"
'It is just a joke.' Life as I knew it came to a screeching halt.': Woman discovers husband's infidelity, worked together to 'fix' what had been broken, 'trust can be restored'
"4 years today we have been married. I will never forget. Discovering a secret that would break me to my core. I lay on the bathroom floor, crying my heart out. How did we get to this point? Not only do I have to handle life with 2 children with special needs, but now this? I am strong for taking the route I have, choosing not to give up."
'Let's do it. Tell them yes!' I begged him. She was so fragile, perfect, small, like holding a doll.': 25-year-old 'mama to many' discovers she's pregnant while fostering, 'What! Seriously?!'
"I'm only 25. Married with two kids at 23. And boom, surprise! We were pregnant again! My husband was downstairs playing on his phone… relaxing. 'Babe….' Silence. The look on his face – pure disbelief. I get a call. 'We have a 3-month-old, very preemie baby girl, ready to be discharged and on oxygen.' My first thought was, 'There is no way we can handle this.' I knew my husband wasn't going to be on board."
'15 years ago, my first daughter was born. She was our 'princess.' But 11 years ago, God gave me Princess.': Woman shocked how sponsorship of little girl 11 years ago would change her life, 'I am forever thankful'
"She wasn't my first daughter, or my second, or my third. My first picture of her showed a scared, sad, little toddler. And for several years after, she still had a look of sadness on her face. Because she was so young, her mother wrote letters to me about Princess, and I'd write letters back. But in October, she wrote my favorite letter. My biggest prayer for her had been answered."
'You reached this point. There is no turning back. But are you kidding yourself?! Is this sustainable?': Gay man marries 'close friend,' comes out to her years later, now happily lives in mixed-orientation marriage
"I knew my same sex attractions were not going away. I knew I was hurting myself. I knew I was hurting Aleesha and the kids. But it continued to be a secret. I never got caught. Until I came clean. I bawled. 'So what now? Do you want me to leave?' We were both sitting there crying, holding each other for dear life. I was surprised to hear, 'No, never. You are my best friend. I need you to stay. We will figure it out.' I expected to be kicked out. Yelled at. But none of that happened. I was shown love."
'His mom delivered another baby. She abandoned him at the hospital.' It was a call we never expected.': Woman adopts teen, his younger sibling born addicted to drugs, 'Does this sound crazy? I think it does too'
"'Have you been notified of that boy's roommate at the group home?,' he asked. 'We have not,' I replied. 'Would you be willing to meet him?' 3 months later, he was moving in with us hours before his first day of high school. We found out I was pregnant by surprise. 'This is crazy. We can't do this, right?' We were going to have 2 babies and a teenager with PTSD. Less than a year ago, we had no kids."
'I'm not scared to die, I'm scared to be forgotten. And I'm worried about you.' I had no words, just tears.': 28-year-old widow gets sign from husband in heaven, 'I'm okay Mama Bear, I made it, I'm now pain free'
"He was slowly beginning to wake. I couldn't wait to see his big brown eyes. I wasn't expecting the first thing out of his mouth to be, 'So, how did things go?' I still hadn't come up with the best way to tell him. I knew I wouldn't be able to lie. The room once again was silent. 'Kyle, I love you. We will get through this together.'"
'Something is wrong. I'm scared, is everything okay?' He told me to stop being so paranoid.': Woman urges 'trust your instincts,' wishes she had before husband murdered
"My husband spent months telling me I was just hallucinating, that I was crazy. I'd run out to his car as he was leaving for work. Something felt very wrong. I later found out, I wasn't crazy. Detectives shut the door after telling me of his death. I was humiliated I found out the truth the same night everyone else did."
'This is just my daycare. My mom is coming back for me.' She never came.': 22-year-old finally adopted by 'forever family' after years of trauma, abuse in foster homes
"The last time a police officer came to my house, he asked, 'Do you want to go get a cookie?' I was only 5, so that was a big deal. I didn't understand why my biological mom was crying as we drove away. We picked up my sister at school, then waited at DHS to drive us to a foster home. All we had were the clothes on our backs, and even those were dirty. 'I need a place to stay until I figure out where to go,' I said."


text:
In a Premier League recreation of three fiercely-contested penalties, Joshua King scored one and missed one other as Bournemouth drew 1-1 on Saturday with Wolverhampton Wanderers who additionally netted from the spot.
Soccer Soccer – Premier League – AFC Bournemouth v Wolverhampton Wanderers – Vitality Stadium, Bournemouth, Britain – February 23, 2019 Wolverhampton Wanderers' Raul Jimenez celebrates scoring their first objective with Ruben Neves as Bournemouth's Nathan Ake reacts. REUTERS/David Klein
Referee Roger East was solely named as officiating final week after stepping in as last-minute alternative, however he ended up taking centre stage along with his selections.
First he awarded Bournemouth a penalty on 16 minutes when King, who went on to attain from the spot, fell beneath minimal contact from Joao Moutinho.
Moments later East once more infuriated Wolves, who claimed they need to have been awarded a penalty when Chris Mepham headed on to his personal arm.
However the guests had been finally awarded a spot-kick when the referee harshly penalised Adam Smith for a problem on Raul Jimenez, who fired residence the equaliser.
Referee East was once more again within the highlight when he awarded a 3rd penalty for Ivan Cavaleiro's problem on Ryan Fraser though TV replays urged contact was outdoors the world.
This time King hit the submit and Bournemouth additionally went shut by means of Jordan Ibe, who crashed a shot in opposition to the bar, and Dominic Solanke, who ought to have executed higher when free on objective.
Bournemouth's Jefferson Lerma was lucky to solely obtain his 10th reserving of the season for elbowing Moutinho within the face and can serve a three-match ban.
Regardless of the controversy, neither supervisor criticised the referee.
"It's a very troublesome process for referees. I don't wish to speak about VAR, so long as the officers are honest no one can complain," Wolves supervisor Nuno Espirito Santo mentioned.
"You can't decide something from the response of gamers to incidents."
Bournemouth's supervisor Eddie Howe mentioned: "My preliminary response to the primary one was that it was a penalty. You're all the time barely biased, however I believed theirs was harsh on us.
"The third one, there's a debate over whether or not it's contained in the field. I haven't seen it once more. We must always rating and win, Joshua King has been a dependable taker for us but it surely wasn't to be."
For all Bournemouth's poor away type, they haven't misplaced within the league to anybody outdoors the highest six at Vitality Stadium this season and moved into 10th place, six locations and two factors behind Wolves.
Reporting by Neil Robinson, modifying by Ed Osmond


text:
Contents Cleaning
Did you know the contents of your home or office can be restored after a flood or a fire? Just as we can restore your property to its pre-disaster condition, we are fully equipped to handle the cleaning of your hard and soft contents. You would be amazed at the number of items affected by flood or fire that can be restored by Crossroads Property Restoration, LLC. After a disaster, it is hard enough dealing with property damage. On top of that, damage to personal possessions can be just as devastating when something meaningful is harmed. We can bring back life to what you thought lost.
Call For Free Quote
Why Call Crossroads Property Restoration, LLC?
The water and fire restoration experts at Crossroads Property Restoration, LLC will even work with you and your insurance company to evaluate the items that have been damaged and come up with a plan to save and restore your possessions. Our process is simple, yet effective.
- Items will be removed from your property and brought to our off-site facility
- All items will be cleaned, repaired, and restored
- After your property is restored, your newly cleaned items will be moved back in
Restoration is Cheaper than Replacement
We have a track record of restoring more than 80% of damaged items. Call us for a free quote on cleaning your damaged content and find out how inexpensively items can be restored to original condition. Just some of the items we regularly restore include:
- Jewelry
- Furniture
- Artwork
- Family photos
- Sports memorabilia
- and more!
Expert Contents Cleaning by Crossroads Property Restoration, LLC
The content cleaning experts at Crossroads Property Restoration, LLC utilize the latest cleaning system technology in our state of the art contents cleaning facility. Items are stored in our climate controlled facility and monitored throughout the process. We hope that you'll never need our contents restoration services, but if you ever do, we are standing by to help.