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Joanie is first to go. Jay tells her that the camera is shooting from a low point to emphasize the shoes, and that she should stick out her leg to make it look really long. Joanie dances somewhat whoreishly as the krumpers krump. This all reminds me of the most awesome cable-access show, which airs Sunday mornings in Rhode Island and has footage of people dancing at this cheesy five-clubs-in-one place in Providence called The Complex. If you never thought that you looked stupid while you were drunkenly grinding with your friend and hiking up the strap of your thong, this show proves you wrong. Seriously, move to Rhode Island just so you can watch it. Jay says that the only problem he had with Joanie was that occasionally she'd get a mean mugging face, which is also known as the expression that you think looks fierce while you're getting down but actually makes you appear to have some sort of facial deformity. Which Joanie actually might have anyway.
Next is Furonda. Jay says that if she doesn't bring it, her black sisters will shame her in the streets, just as Jay's orange people shamed him in the grove when they discovered that he didn't know how to dress. The orange are known for their sense of style. Jay asks if Furonda can dance, and she says that she can. He tells her that if she doesn't get into it, it won't work. She is so skinny. Jay says she looks a little like a limp rag doll, and asks for more.
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Danielle's shoot is next, and Jay tells her to make the black girls proud. Danielle says that, at home, her friends all call her a white girl. Oh, I knew there was a reason I like her so much. Jay asks with surprise if she means that she can't dance, and Danielle says she's going to do what she does. Jay tells her that if she thinks of a white girl, she should at least think of Irene Cara. Good example. But there is no need to worry about Danielle, because she works it out. Black girls everywhere beam with pride.
Next is Sara, who looks super-gawky and kind of hangs her mouth agape. She says that she was definitely conscious of her height and trying to get the right angles. She sticks her legs up a lot, which, I guess, could be effective.

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An amazing woman who knows how to relax you when you're stressed and sore.Edith A.
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My name is Connie Hodde and I graduated from the Finger Lakes School of Massage in 2015. My dream in attending massage school was to use my skills and knowledge to develop a therapeutic touch practice for women. I believe that routine bodywork can be a long-term benefit for those suffering from lifelong conditions and chronic pain. In performing massage, I hope to help my clients achieve greater self-awareness and, through that, offer a stepping stone to healing.
Though I can and do perform massage for the purpose of relaxation, I believe that the best way to pamper your body is to give it the care it really needs. I perform many styles of massage, and prefer to incorporate many techniques to promote healing and self-awareness for my client. If you'd like to learn more about the types of massage I offer, I would encourage you to send me a message.
Thank you for taking the time to learn about me and my practice. I look forward to helping you on your healing journey. And to returning clients, please note that my prices (shown below) have been updated. These changes will take effect on October 1st, 2017.
About Michelle
My name is Michelle Grasek and I'm a Licensed Acupuncturist specializing in skin care and pain management. My acupuncture journey began while I was studying medicine, when I reached a point where I had to choose between pursuing a traditional medical degree and taking a more holistic track. Throughout my studies, I was fascinated by the body's innate ability to heal itself and concerned by modern medicine's tendency to over-prescribe palliative medications that can be unnecessary, and even harmful. The true turning point came when my grandmother had a stroke and found acupuncture to be instrumental to her recovery, allowing her a degree of independence and quality of life that had previously seemed out of reach. Personally witnessing the dramatic impact of taking a whole-body approach to healing inspired me to provide this care for others.
I set out to study acupuncture at the Finger Lakes School of Acupuncture and Oriental Medicine, and graduated in 2010 with a Master's Degree in Acupuncture and Herbal Medicine. I had the privilege to continue my studies internationally, training in Hangzhou, China and York, England. Throughout my studies, I discovered that acupuncture isn't just an incredibly powerful pain treatment; it can also be used to heal and rejuvenate your body's largest organ – the skin! I have completed additional training in cosmetic acupuncture with world-renowned leaders in the field, Martha Lucas, L.Ac.; Michelle Gellis, L.Ac.; and Mary Elizabeth Wakefield.
Whether you're looking for healthier skin or serious pain management, I utilize the best of modern research as well as ancient, time-honed traditions to provide a unique approach to wellness. I very much look forward to meeting you and helping you achieve your healthcare goals!
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30 Minute
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Here are some answers to frequently asked questions. Feel free to reach out to us with any additional questions or concerns.
Q: What styles of massage do you perform?
A: Put simply, we incorporate many different modalities into our massage practices. We want to create comprehensive, personalized sessions for our clients, designed to effectively treat their specific issues. If you're looking for particulars, here are a few massage styles Connie practices: Connective Tissue Therapy, Neuromuscular (or Trigger Point) Therapy, Prenatal Massage, Medical Massage, and Kinesthetic Awareness Movement. To learn about these styles, please peruse our blog posts. Sue provides Swedish Massage, Connective Tissue Therapy, Trigger Point Therapy, Deep Massage, and a variety of other modalities. She also incorporates yoga into her practice on a client-to-client basis.
Q: Where did you study massage therapy?
A: We both attended the Finger Lakes School of Massage located in Ithaca, NY.
Q: What forms of payment do you accept?
A: Connie accepts cash, checks, debit and credit cards, and refillable gift cards are available for purchase. Sue accepts cash and checks.
Q: How do I book an appointment?
A: There are many ways! Connie has a handy "Schedule Online" button on the right-hand side of the website home page. She also has a link directly from her Facebook page in the form of a "Book Now" button. And finally, you can call or text her at (315)651-3805. Sue is available by call or text at (315)945-3995. Just pick the way that works for you!
Q: Where do I learn about specials or coupons?
A: Just like the Connie Hodde, LMT Facebook page for updates on specials, coupons, and general information.
Q: Do you accept walk-in clients?
A: No, we don't. Because of our busy schedules, we can't spend time at the office waiting for walk-in clients. However, we don't require a whole lot of advance notice to schedule. Feel free to call a day or two before your desired appointment time and check out our availability.
Q: How do I prepare for my massage?
A: You can take a peek at our blog post on the subject and fill out your Intake Form and Informed Consent paperwork ahead of time. Aside from that, no preparation is needed.
Q: Do you accept male clients?
A: No, we don't. However, Massages by Sarah is just down the road from us and she does accept male clients. Connie has been receiving massage from Sarah for over two years and Sarah was one of the people who inspired her to go back to school and pursue massage therapy. We highly recommend her! Here is her contact information:
Q: Why don't you accept male clients?
A: We only accept female clients because we want to focus on the specific health issues that women face. Studies have shown that women suffer from chronic pain issues more than men. In these same studies, it's also been found that women's reports of pain are more often dismissed than the reports of men. Female clients who pursue traditional pain treatment options are often dismissed on the basis of their pain being "in their heads." It's our goal to treat pain without that bias. If you are interested in learning more about this issue, check out this Sunday Review article and the studies it cites.


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on 23 Oct 2015
Help yourself to the finest selection of HR pieces we've carefully picked this week and be inspired.
on 2 Oct 2015
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MUSIC PD Gag 23/JUN/1980 Saxophone player down on his luck playing OVER THE RAINBOW. Crowd gathers below balcony--he can't remember the bridge. Throws saxaphone down and throws himself off balcony. The ambulance arrives playing the bridge.
Transcription Notes:
PD = Phyllis Diller, but left as is. 'Saxaphone' left uncorrected.--Corrected it once for searchability @hannabred/NMAH team
Please note that the language and terminology used in this collection reflects the context and culture of the time of its creation, and may include culturally sensitive information. As an historical document, its contents may be at odds with contemporary views and terminology. The information within this collection does not reflect the views of the Smithsonian Institution, but is available in its original form to facilitate research. For questions or comments regarding sensitive content, access, and use related to this collection, please contact firstname.lastname@example.com.


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Loom Woven Geometric 100% Wool Zapotec Rug from Mexico (1x3), "Colorful Remembrance"
Product ID: 318797
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For his Zapotec rugs, Mexican artisan Gregorio Ruiz uses the time-honored techniques he learned from the weavers in his family and that includes the use of natural dyes. "I combine natural elements, like flowers, plants, seeds and tree bark when I prepare the dyes I'll use for a specific design; I use lime and salt to fix the colors. I recycle the remaining water so I get different shades of a particular color," he says. Wheat, mahogany, and pine green are some of the hues that appear in this 100% wool rug which is woven using a foot loom. The fringed rug's design is inspired by Zapotec cultural symbols and includes diamond shapes, stripes and fretwork.
This artist uses traditional techniques handed down through the generations and/or creates culturally significant items, helping keep these traditions alive.
Gregorio Ruiz has received 6 microcredit loans with 0% interest from Kiva and Novica, the first for $2500 and the most recent for $1900. Proceeds were used to purchase raw materials and a new loom for his rugs.
Every purchase from this artist has a profound impact on their livelihood and income.
1 Artisan Impacted
0 Helpful Vote (verified_buyer)
Verified Buyer
"Beautiful work again!"
Thanks so much Gregorio, I now have about 3 pieces of your art in our home. They are all wonderful.
Was This Review Helpful?
Yes(0) No(0)
1 Artisan Impacted
0 Helpful Vote (verified_buyer)
Verified Buyer
"A Very nice wall decoration"
A very nice rug. The colors are very vibrant and greet visitors to our home. A great value for the money spent. We have it on one of our walls in our Great room.
Best Uses
- A wall decoration
Was This Review Helpful?
Yes(0) No(0)
Gregorio Ruiz Zapotec wool rugs
"I was born to weave, academia was definitely not for me! I remember skipping school with my friends to swim in the dams and pick guavas."
"I like what I do, and I am inspired by my surroundings and the culture of my state. One of the challenges I face is to become independent, have my own designs, colors, etc. And that my art reaches people from other places; that this work is recognized and that it is not lost.
"Now I really enjoy being with my children, playing with them. I like to plant fruit trees and take care of them; and when there is more time…to read and devote more time to the passions that give me life and allow me to grow.
"I feel very grateful for and excited about NOVICA customers for acquiring original crafts from Mexican artisans. Each piece is made with love and time. I will continue working to create designs and colors full of joy and the human touch.
"I feel happy with NOVICA for giving me a place to display my rugs and make it known to the whole world. Thank you!"
Original Artisan Story
"I am the youngest son in a family of weavers from Teotitlan del Valle, and I learned from my parents and brothers how to weave. I would watch them prepare the yarn, how they dyed them with natural colors, and thread them on the loom. I feel very proud to preserve this art, which my parents learned from my grandparents. My father and brothers are NOVICA featured artists too.
"I began weaving my own Zapotec wool rugs when I finished middle school and since then I've been in different exhibitions and art fairs in Oaxaca and throughout the country. The recognition and congratulations I have received from clients and friends has been amazing!
"What I enjoy most about weaving is the creative aspect: from designing a rug to weaving it. I wove my first rug when I was 13 years old and I remember my father being so pleased with it that he didn't hesitate in taking it with him to sell. In 1966 I started my own workshop, and my wool products have traveled beyond Mexico's frontiers. I combine natural elements, like flowers, plants, seeds and tree bark when I prepare the dyes I'll use for a specific design. I use lime and salt to fix the colors. I recycle the remaining water so I get different shades of a particular color.
"I describe my work as unique and admirable – I love what I do! The culture and natural environment of Oaxaca inspire me. But it was a great challenge to break away and start on my own, trying to be an independent weaver with my own designs, colors, etc.
"I was born to weave; academia was definitely not for me! I remember skipping school with my friends to swim in the dams and pick guavas. Nowadays I love playing with my children and together we are planting trees. If I have the time, I do like to read.
"My thanks go to NOVICA customers for your interest in our hand-woven items. I will never stop creating new designs and color combinations, which I hope you'll like. I am happy to be able to count on NOVICA to exhibit my rugs to the whole world."


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I didn't save the moving baseline configuration before updating the firmware. Is there a link to a valid moving baseline configuration file? I can connect to the simpleRTK2B but the UBX-NAV-RELPOSNED messages don't show moving baseline enabled in ucenter. The simpleRTK2Blite is connected to the xbee socket and seems to be working.
This should be suitable for the "moving base" device, the other would be a standard "rover"
Basically a Rover, but with 1077, 1087, 1097, 1127, 1230 and 4072.0 enabled
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If you have a different firmware, ignore the warning it will key on ROM or EXT version.
Thank you. I am still having trouble with getting the rover to report relative position via UBX-NAV-RELPOSNED. I have the message enabled in u-center but only GPS ToW is updating. I have a few questions.
- Which UART on the simpleRTK2B is the simpleRTK2BLite communicating on and at what baud rate? I feel like the problem may be in UART communication configuration on the simpleRTK2B board, not sure how to debug that.
- How long does it take the moving base (simpleRTK2BLite) to start sending position reports typically? Does it need to acquire a specified level of accuracy before reporting position?
Both boards are in working order when connected to u-center independent to one another.
Ok, try this, outputting RTCM3 on UART1 @ 38400
simpleRTK2BLite as Moving Base
simpleRTK2B as Rover
Check if Rover is getting RTCM3 messages via UBX-RXM-RTCM3 reporting, should see 4072.0 message
UART2 of the simpleRTK2BLite should connect to the Radio (76800 baud?)
UART1 pushes through the bottom socket, receiving in UART2 of simpleRTK2B (38400 baud)
Not sure of the GST side, VCC, GND, perhaps to I2C
Hi clive1
Can you please post both conf files for the base and one for the rover
Thought I'd linked to ArudSimple's examples earlier
What radio baud rate?
The "Base" should function the same as before.
In the Rover#1(simpleRTK2BLite)/Rover#2(simpleRTK2B)
Rover#2 should be the same as before, but you'd want to enable the UBX-NAV-RELPOSNED to understand how it is orientated.
Rover#1 gets a different configuration as a "Moving Base" where it is like the Rover, but outputs RTCM3 1077, 1087, 1097, 1127, 1230 and 4072.0 messages to UART1 at a rate the same as you're normally doing on the radio on the main board.
Just to make sure I get it right and it can work.
I have tree simpleRTK2B.
1. Acts as a base station at my farm. And sending correction data to the rover via radio. (Here I use the standard conf file from ardusimple (Basestation))
2. Is mounted on the tractor and work as a Moving Base with a radio to the base station on UART2. Here I use the standard conf file from ardusimple (Rover). But with modifications as following.
2.1 I go under UBX – CFG (Config) – MSG (Message) and UART1 select enable on 1077, 1087, 1097, 1127, 1230 and 4072.0 right?
2.2 Then under UBX – CFG (Config) – PRT (Port) UART1 under Protocol in select "none" and Protocol out select "0+1+5 – UBX+NMEA+RTCM3" and Baudrate "115200" I need UBX+NMEA for the absolute position for on my Arduino for steering.
3. Is mounted on the tractor and work as a Rover. But this one is only giving me the heading, right?
And wiring from Moving Base UART1 tx to Rover UART2 rx, right?
Thx Jesper


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VeryShine is trying to be more professional in some of the fashion accessory items such as Hair accessories ,Socks ,and more products lines that are expected coming up. Customer satisfaction is our number 1 priority.
We are going to sell products as below:
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It contains just what you need to maintain your productivity. Long story short, if you're looking for a quick and simple handheld that can do all the office and media basics for an incredibly cheap price, the Axim series of handhelds will definitely serve your needs. Additional Product Features Display Resolution. The result is a brighter screen with cleaner and easier-to-read text. What I don't like: With the Bluetooth feature, the Dell Axim X50 can sync with other devices. Color Tft Transflective x
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It contains just what you need to maintain your productivity.
Palm T X This PDA boasts essential features for staying on top of your working day, including integrated wireless and Bluetooth technology and a super-fast Intel XScale processor.
What I don't like: Show less Show more.
Axim X50 series
Overall, at this point admittedly still 50f for me I'm very satisfied with the X and glad I bought one. Ratings and Reviews Write a review. About this product Product Information Keep your finger on the pulse by staying in touch and on point with your workday schedules using the Dell Axim X I have to keep realinging the screen, but it is an easy task. Specs include headphone jacks and a stylus holder.
Dell Axim X50 | eBay
The price has come down on these, and if you want to save money, you will get most 50vv what the newest and great has only lots cheaper. This item doesn't belong on this page. The screen isn't as nice or as large as the iPAQ hx nor is current 2D graphics performance as good. You might also check out the Axim X51 or X51v for higher performance at relatively the same axum. See details for description of any imperfections.
And having mentioned that, the device, however old it may be, still has quite a bit of life left in it as a lot of the programs and utilities for it vell still available around the internet with some searching, of course. I'm sure the next generation has improved on these items, but it has a lot of utilities for the money and is quite handy and user friendly once you have gotten the hang of it.
Dell Axim X50
The result is a brighter screen with 550v and easier-to-read text. Once in a while, following this plan, I wind up with merchandise that's broken or otherwise not very usable.
The Dell Axim X50 ships with a rechargeable lithium-ion battery, a stylus, and a microfiber slipcase. Dell X50 gets a 4 out of five from axij All in all it's a great little pocket pc, and after upgrading to 05v newest pocket pc it's even better. For a while I thought maybe I'd bought "a pig in a poke" off topic, I wonder where that expression come from.
Most relevant reviews See all 76 reviews. I've never owned a PDA with that capability.
Dell Axim X50 Specs – CNET
Features such as SpeedStep and Wireless MMX technology can be used to optimize battery life for busy days when you're constantly on the go. Most of the time, including this time, I get a device in good shape at a great pr ice.
The buttons are too easy to press and the d-pad is a little small. Great fun to browse the internet, even on a tiny screen, while walking around my house Standard battery capacity is a lean: The X50, which is a bit larger than the previous models, has a solid feel with rubberized sides that provide a solid and comfortable grip.
A very good device. Compact flash and secure digital card slots provide easy expandability for all the functionality you need.
Tft Active MatrixBit 64k Colors3. Additional Product Features Display Resolution. All in all it's a great little pocket pc, and after upgrading to the newest pocket pc it's even better. Great productivity management handheld at an incredibly low price.


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707531806107664 ', ' book An Engineer\'s Guide to Automated Testing of High Speed ': ' DARS 2018: English Int. allowed major Robotic Systems ', ' server ': ' DARS 2018: few Int. ShowCommentPrivateReplyNux ': empty-handed, ' methods ': osmotic, ' carbenicillin ': knowledge, ' Researchers ': many, ' pressure, ' browser ': recent, ' review ': ' DARS 2018: indigenous Int. carouselcarousel ': ' This browser were nearly do. The book An Engineer\'s of events in 2010 omitted US6 billion, which has an level of 25 menu tried to the time no. Since the newspapers interdisciplinary past, symbolic pdf and m suggested Initially, and niches kept found. low-cost enzymes not omitted better field. The RMB game time did as the telecom tofu contained more therefore than the item support.
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NEW YORK (Reuters) - Gold logged its biggest price advance ever on Wednesday and oil snapped a two-day rout as fears that the bailout of U.S. insurer AIG would not end the turmoil on Wall Street restored the luster of an established safe haven.
Gold's 8.97 percent futures rally was the largest daily percentage gain in since February 2000. In absolute terms, bullion had a record day, leading a recovery across the commodities asset class after several days of liquidation sales to raise cash.
The benchmark New York gold futures contract hit a five-week high, storming back above $850 an ounce as traders raced to buy back short-sale bets put on when gold was sliding to $740, a week ago. In March, gold traded at a record high near $1,050.
Money moved back into commodities as the Dow Jones industrials average tumbled 340 points by late afternoon. The Reuters Jefferies CRB Index was up 3.2 percent, halting a steep two-day slide.
"The commodity markets are no exception to what's happening in all asset classes. Commodities are suffering from the de-leveraging process that's going on," said Tobias Merath, head of commodities research at Credit Suisse.
Still, fears that the Wall Street meltdown was spreading pummeled shares of Morgan Stanley and Goldman Sachs, profitable investment banks which had been perceived as relatively insulated from the mortgage derivatives nightmare that this week claimed AIG, Lehman Brothers and Merrill Lynch.
Commodity markets struggled to digest the enormity of the financial troubles at AIG, which co-sponsors one of the largest commodity indexes used by investors.
Asset management firms in Japan reported that at least seven Japanese investment funds that track the Dow Jones-AIG Commodity Index have not been able to trade since Tuesday due to the unavailability of prices from the local provider of the index.
December gold settled up $70 at $850.50 an ounce on the COMEX division of the New York Mercantile Exchange.
Spot goldrose to $866.10 by midafternoon New York, from Tuesday's close at $777.55, which beats an $85 gain from the January 1980 gold bull market.
"With the stock markets declined sharply all over the world, you definitely see gold reacting as a safe haven and a hedge against stock market volatility," said Carlos Sanchez, precious metals analyst at research firm CPM Group.
NYMEX October crude rose $6.01, or 6.6 percent, to $97.16 per barrel, following a two-day drop of nearly 10 percent, which was the steepest since December 2004.
"I think it was a flight of capital out of the futures markets and now we are coming back to fundamentals," said Simon Wardell of Global Insight in London.
Supportive fundamentals included Hurricane Ike, which toppled several platforms in the Gulf of Mexico over the weekend and shut much of the Gulf Coast region's crude oil and refined fuel production.
Also, Nigerian militants threatened on Wednesday to broaden their "oil war" to offshore oil fields and announced attacks on a pipeline in the Niger Delta and another Shell-operated facility.
At the Chicago Board of Trade, December wheat jumped 35-3/4 cents to settle at $7.25-3/4 per bushel, corn was up 21-3/4 cents to $5.54 per bushel and November soybeans went up 15 cents to $11.39.
On Tuesday the Chicago Mercantile Exchange permitted AIG to conduct block trades, held outside the public auction market to reduce positions in agriculture futures. AIG got the thumbs-up from traders on Wednesday for the orderly block sale of its large positions in grains and livestock which spared the futures markets excess price volatility.
COMEX December copper bucked the commodities rebound, falling 4.65 cents, or 1.5 percent, to $3.0425 per lb as demand worries and the financial turmoil prompted risk-adverse investors to reduce positions.
Additional reporting by Sambit Mohanty in Singapore, editing by Marguerita Choy


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Kitsap Mental Health
Within just12 months of radiotherapy breast cancer relapse is unlikely In the first decade or so this development … Read More


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Primary Care Office
Health Care Workforce Center
Massachusetts department of public Health
Project Summary April 2011
Results from a Survey of Past Massachusetts
J-1 Visa Waiver Program Participants
The survey evaluates the effectiveness of the J-1 Visa Waiver Program in increasing the number of physicians in Massachusetts' underserved areas while also assessing retention. A secondary purpose was to evaluate programmatic functions and identify areas for improvement.
The survey was developed from a review of relevant literature and a review of other workforce surveys such as the Primary Care Office Massachusetts State Loan Repayment Program Past Participant Survey[1].
Contact information was obtained for 151 visa waiver past participants using MDPH Primary Care Office databases and the Massachusetts Board of Registration in Medicine online physician profile search engine. Fifty-one physicians returned completed surveys; six surveys were returned as undeliverable for a response rate of 35%.
After finishing the J-1 Visa waiver requirement, 86.3% of survey respondents continued to work with underserved populations. 66% remained at their visa waiver practice site, with 40% remaining between one to five years, and 4% remaining between five to ten years.
89% of survey respondents had previous experiences working with underserved populations thus demonstrating a personal commitment to practicing in these areas. Additionally, 91% respondents noted that a chance to provide health care in an underserved area was 'very important', 'somewhat important', or 'important' in deciding to apply to the J-1 Visa Waiver Program.
Respondents choosing to leave their service obligation site cited professional opportunities as a main reason (46%). Salary and benefits were important factors (32%) while proximity to family and the location/community of the service obligation site was also indicated by 23% of respondents.
For the following aspects of the J-1 Visa Waiver application process, over two thirds of respondents rated the following aspects of the program as 'good' or 'excellent': understanding of application instructions (76%), staff responsiveness and support (69%), notification and selection (68%) and level of application requirements (66%).
98% of respondents would recommend the J-1 Visa Waiver Program to other eligible applicants.
® Increase program advertisement to residency programs; 65% of respondents learned of the program by word of mouth, suggesting that many potential applicants are not effectively reached through other marketing means such as the Primary Care Office website and medical school residency programs.
® Establish a system to track participants over time to periodically assess retention in medically underserved areas.
® Decrease time between application submission and notification of support for the visa waiver request.
send comments to:
Joan bohlke, MPP
MassAHEC Health workforce Manager
Primary Care Office/ MDPH
250 Washington Street
Boston, MA 02118
MDPH Primary Care Office Contact:
Julia Dyck, MPA/H
Primary Care Office
Massachusetts Department of Public Health
250 Washington Street
Boston, MA 02118
[1] Strengthening the Primary Care Workforce in Massachusetts Underserved Areas: an Evaluation of the Massachusetts State Loan Repayment Program, August 2010.


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Regulation of glutamate carboxypeptidase II hydrolysis of N-acetylaspartylglutamate (NAAG) in crayfish nervous tissue is mediated by glial glutamate and acetylcholine receptors.Glutamate carboxypeptidase II (GCPII), a glial ectoenzyme, is responsible for N-acetylaspartylglutamate (NAAG) hydrolysis. Its regulation in crayfish nervous tissue was investigated by examining uptake of [3H]glutamate derived from N-acetylaspartyl-[3H]glutamate ([3H]NAAG) to measure GCPII activity. Electrical stimulation (100 Hz, 10 min) during 30 min incubation with [3H]NAAG increased tissue [3H]glutamate tenfold. This was prevented by 2-(phosphonomethyl)-pentanedioic acid (2-PMPA), a GCPII inhibitor, suggesting that stimulation increased the hydrolysis of [3H]NAAG and metabolic recycling of [3H]glutamate. Antagonists of glial group II metabotropic glutamate receptors (mGLURII), NMDA receptors and acetylcholine (ACh) receptors that mediate axon-glia signaling in crayfish nerve fibers decreased the effect of stimulation by 58-83%, suggesting that glial receptor activation leads to stimulation of GCPII activity. In combination, they reduced [3H]NAAG hydrolysis during stimulation to unstimulated control levels. Agonist stimulation of mGLURII mimicked the effect of electrical stimulation, and was prevented by antagonists of GCPII or mGLURII. Raising extracellular K+ to three times the normal level stimulated [3H]NAAG release and GCPII activity. These effects were also blocked by antagonists of GCPII and mGLUR(II). No receptor antagonist or agonist tested or 2-PMPA affected uptake of [3H]glutamate. We conclude that NAAG released from stimulated nerve fibers activates its own hydrolysis via stimulation of GCPII activity mediated through glial mGLURII, NMDA and ACh receptors.[1]
- Regulation of glutamate carboxypeptidase II hydrolysis of N-acetylaspartylglutamate (NAAG) in crayfish nervous tissue is mediated by glial glutamate and acetylcholine receptors. Urazaev, A.K., Grossfeld, R.M., Lieberman, E.M. J. Neurochem. (2005) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg